LOCUS       DAOQPA010000001       100823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_59_length_100823_cov_20.473970, whole genome shotgun sequence.
ACCESSION   DAOQPA010000001 DAOQPA010000000
VERSION     DAOQPA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 100823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 100823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100823
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_100823_cov_20.473970"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PKX01_00005"
     CDS             <1..1469
                     /locus_tag="PKX01_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ74531.1"
                     /translation="RFFPAWGQARLHAMIANRPDWCISRQRNWGVPIPFFLHKATGEL
                     HPRTVELMEAVATRVEQEGIEAWFKLDPAELLGDEAAQYDKISDTLDVWFDSGTTHWH
                     VLRGSHPDGHATGPRADLYLEGSDQHRGWFHSSLLTGCAIDGHPPYHALLTHGFAVDG
                     SGRKMSKSLGNVVVPQEVTGKLGAEILRLWVASTDYSGELSISKEILDRVVEVYRRLR
                     NTLRFLLANTADFNPATDMLPVDQWLEIDRYALALTRRLQQQAEADYARFEFHRVVQA
                     LQNFAAEDLGAVYLDILKDRLYTTAATSAARRSAQSALWHILQAVVKLMAPILSFTAE
                     EIWLLLGKDADDSVMLHTFHTLPTQDGEDALLARWEAIRAVRAEVMKQIEAVRSDGKL
                     GSSLQAEVELRAAGAQYDLLASLGDDLRFVLICSKTVLTRVDSEADAAIVVSPSAQRK
                     CERCWHYRDDVGHDPAHPELCGRCTSNLHGTGESRAHA"
     gene            1491..1985
                     /gene="lspA"
                     /locus_tag="PKX01_00010"
     CDS             1491..1985
                     /gene="lspA"
                     /locus_tag="PKX01_00010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HMZ74532.1"
                     /translation="MKLRLGNWIGLAAVVVLLDQWTKHLVRGALRTGEVIPVTDFFDL
                     VLAFNPGAAFSFLADQAGWQRWFFVALAAVICGWLLRLVARHQHEFLQPLAFSLIIGG
                     AIGNVIDRFVFGAVVDFLYFHVGRYGWPAFNVADSAICVGVTLMVIAQLREHRHAPTR
                     ETLS"
     gene            1982..2413
                     /locus_tag="PKX01_00015"
     CDS             1982..2413
                     /locus_tag="PKX01_00015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ74533.1"
                     /translation="MTQTVQPDSLVTLHYRISLDNGQPLISTFEAQPATMHLGQGDIA
                     PTLERCLAGLAVGERHSFELAPENAFGAHRAELVEKVRLEDFPDGAEVEPMVIMEFTA
                     PDGSRYPGLIREVLDTHAVIDFNHPLAGKPIRFEVQIIGIN"
     gene            2421..3353
                     /gene="ispH"
                     /locus_tag="PKX01_00020"
                     /gene_synonym="lytB"
     CDS             2421..3353
                     /gene="ispH"
                     /locus_tag="PKX01_00020"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HMZ74534.1"
                     /translation="MEVLLATPRGFCAGVERAIEIVERALARFGAPIYVRHEVVHNRF
                     VVEGLRAKGAIFIEDLADVPAGNTVIFSAHGVPQSVRSEAEARGLRVFDATCPLVTKV
                     HLEVARMRDQGREIVMIGHKGHPEVEGTMGQVKDGIHLVESVGDVATLQVNTPDALAY
                     VTQTTLSVDDAAAIVGALRERYPAIVGPKKDDICYATQNRQDAVKFMAPQSDLVLVVG
                     SPNSSNSNRLREVAELLQVPAHLVDNADAIDPAWLTGKQRIGVTAGASAPEVLVESVI
                     ARLKALGAASVRQLEGVPEKVTFPLPRELQTPAA"
     gene            3439..3771
                     /locus_tag="PKX01_00025"
     CDS             3439..3771
                     /locus_tag="PKX01_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74535.1"
                     /translation="MSFVQRNAEGCIVALFADAGPGHSEELPADHPEVAAFLGGRGAA
                     DGAVTFTALDADLIRVIEDVVDVLIDRGLLRLTDLPPDAQRKLLARKGARERLRNGLN
                     LLGKHDVI"
     gene            3924..6086
                     /locus_tag="PKX01_00030"
     CDS             3924..6086
                     /locus_tag="PKX01_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HMZ74536.1"
                     /translation="MTNPTSRSGWQIGEHATHDDPLLDALVLLTRIHGNPFTPEALSA
                     GLPLVDHRLTPSLLPRAAARAQLTARIVRKPLAEITPNFLPALLLLHDRRACLLLERL
                     PDGACRVRFPEAGESVDVLSADELAARYTGLVCFVRPRFRFEARAPEIRSLRGQHWFW
                     GTVLDNWRLYRDTLLAALVINLFALAIPLFSMTVYDRVVPNRAVETLWVLAVGTALLL
                     CFDFALRTLRAYIVDTAGKRIDVQLSARIMERVLGLRMEARPASVGSFAANLRAFEGV
                     RDFIASATVTTLIDLPFVLLFLLAMAWISPWLMIPPVVGILVVLFVTLIAQDKMHALT
                     ETTYRAGAQRNATLVESLVGLETLKTLAAEGLIQRQYERATLFIAQIGGRLKLLSAGT
                     VNFAQFMQQFVSVAVIIVGVYELADAKMSLGGIIAASMLAGRTMAPLGQVAGLLMQYH
                     NARTALETVEGHMQLPVERPDGANFVHRPAFRGEIEFKDVSFAYPGRDQAALHTISFR
                     LQAGEKVAIIGRIGSGKTTIEKLILGLYQPTAGAVLIDGIDARQVDPAELRRAIGFVP
                     QDVTLFYGSLKQNIAMGAPFADDAAVLAAAELAGVKEFADAHPQGFDMPIGERGESLS
                     GGQRQAVAIARAVLNDPPILLLDEPSSSMDHQSEEQLKQRLRAFAAGKTIVLVTHRTS
                     LLDLVDRLIVLDQGHIVADGPKAQVVEALQQGRIGRGG"
     gene            6091..7509
                     /locus_tag="PKX01_00035"
     CDS             6091..7509
                     /locus_tag="PKX01_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HMZ74537.1"
                     /translation="MKSLFARLAAFIQPQYARVYDRSSRAFDRLLDGAERGEIHDDTD
                     YLADAEWAMAEQRLRGSRIAVWITLVAVLVLLLWAAFAQLDEVTRGEGKVIPSKQVQI
                     LQSLDGGIVSEILVKEGQQVKAGELLLKVDPTRFVSSLRENRAQYLALLAKAARLTAI
                     SHGKPFVAPPEVLKDAPDLVEQERSAYESKRAEMDAAASIARQQLSQRRQELAEVSAR
                     RDQSSQSYDLTARELEVTRPLAKTGAVSEVELLRLERDVARFKGERNAAVAQIPRIQA
                     AIAEAQNKVEEVELQIRNQARSELSDTNAKIAALSAGSEGLADRVKQAEIRAPMNGTV
                     KQLFANTVGGVVQPGKEIIEIVPSDDTLLLEARVQPKDIAFLRPGQPALVKFTAYDFA
                     IYGGLDATLEQIGADTITDEKGNAYYVVRVRTVHSALGDARLPIIPGMVAEVDILTGK
                     KSLLSYLLKPVLRAKANALTER"
     gene            complement(7600..8487)
                     /gene="galU"
                     /locus_tag="PKX01_00040"
     CDS             complement(7600..8487)
                     /gene="galU"
                     /locus_tag="PKX01_00040"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HMZ74538.1"
                     /translation="MKKVTKAVFPVAGMGSRFLPATKASPKEMMPVVDKPLIQYAVEE
                     AVAAGMTDLVFITGRSKRAIEDHFDKAYELETELEARGKTELLKIVRETVPANVNCIY
                     IRQPEALGLGHAVLCAAPVVGDEPFAVLLADDLLDGQGASSVMRQMADVYHYHRCSVL
                     GVMNVPREHTRQYGIVSTERQDGDVQRVTGIVEKPKPEEAPSTQAVVGRYVLTGRIFE
                     HLRKLTPGAGGELQLTDAIASLLREEAVLSYQFKGVRYDCGSKLGYLQATVELGLKHP
                     EVGAEFAAYLASRAGVDGA"
     gene            complement(8484..10601)
                     /gene="ligA"
                     /locus_tag="PKX01_00045"
     CDS             complement(8484..10601)
                     /gene="ligA"
                     /locus_tag="PKX01_00045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMZ74539.1"
                     /translation="MSVPAEQIARAAALREALERYNHAYYVLDEPLVPDAEFDARFRE
                     LQALEATYPELLTPDSPTQRVGGQPAPELAPVSHAVPMLSIRTETDTTAEGAVVFDTR
                     VRRALGLTDDAPPVTYAAELKFDGLAINLRYEGGTLVLAATRGDGTTGEDVTRNVRTI
                     RQIPLRLKGALPPVLEVRGEIYMRRDAFDRLNADQAAAGQKTFVNPRNAAAGSLRQLD
                     PAITARRPLSFFAYGLGEVVGWELPPTHAELLDALDALGFPVCVHRTRAQGAAALAQF
                     HEAMGVLRDGLPFDIDGVVYKVDSLALQRALGFVSREPRWAVAHKYPAQEAVTYLLGI
                     DVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQGEIDRKELRVGDRVVVRRAGDVIP
                     EVVGRVIGNEEEEAAHVARPRFDLLGTYPVCPECGSHVVRGEDEAVARCTGGLYCPAQ
                     RKQALLHFAGRRAIDIDGLGEQIVHQLVTNSTVTTPADLYNPDRINANVLAGLERMGE
                     KSAANLMAAIEASRETSLARFIFALGIRNVGEKTAKDLARHFGGLKALMAADEAQLLQ
                     VPDVGPVVARCIVEFMGEARNQAVIGSLADALHWTEGAPAARSADGGLAGKTFVLTGT
                     LPTLKRDAAAALIEAAGGKVSGSVSKKTDYLVAGAEAGSKLDKAQQLGVMILDEAGLM
                     ALLEPVPPAQDGGGQGSLFEDQE"
     gene            complement(10611..11687)
                     /locus_tag="PKX01_00050"
     CDS             complement(10611..11687)
                     /locus_tag="PKX01_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HMZ74540.1"
                     /translation="MSELQMGLIGAGGAVVLGIFAFNKWQERRQRKLAEPPVPTEPQA
                     SGTAARGGEARIEPVLGAATPRPVAARPAPTAGPRRVPPPLPAEVDERADCVVRVESI
                     EPVQGSRLLQAAAQHLQGIAKPVRWFGFSDHDNRWETLDGAPGHGYHWICAALQLVDR
                     RGPVSEGDLSAFCGGLQRMCDIFMGIPSLPVKAEALDRATELDRLCAGVDIQIGVNVV
                     ARELPIPGTKLRGLAEAAGLELGTDGCFHARDDLGLSQFVLSNLEPVRFVAEEMRGLS
                     TQGVTLTLDVPRVADGVKVFDRMMTVAQQLAEALNGAVVDDNRTPFGDKAAGLIRAQI
                     EQFQVQMGEQGIAPGSTLARRLFA"
     gene            complement(11715..15236)
                     /gene="smc"
                     /locus_tag="PKX01_00055"
     CDS             complement(11715..15236)
                     /gene="smc"
                     /locus_tag="PKX01_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMZ74541.1"
                     /translation="MRLAKLKLAGFKTFVDPTTVLTPGNLVGVVGPNGCGKSNIIDAV
                     RWVLGESRASALRGGSMLDVIFNGSTTRKPVSRASVELVFDNAEGRAAGQWKPYAEVA
                     VKRVLDRSGESTYFINGLQVRRKDVIDLFLGTGLGPRAYAIIEQGMISRIIEARPEEV
                     RGFLEEAAGVTKYRERRKETEGRLTDARDNLARLEDIRHELGQQIGRLEAQAAVAERY
                     HRLNASLSERQNLLWLIKRNEARSERTRLTEELGEATRRIEADSARLQTLESQLESAR
                     EAQFAASGHVQHAQDALHGVSTELTRLEGELARLRDSRRVLESRLVQLERDDAQWRSQ
                     LDTLSSDAERWHSLQEHAALRAEQAEARHAELAERLPEAEDALRTADATAAAVRRELN
                     QAEQQIRVEEAHRVSSERTLETLGQRRGRLQQEQSAVDAPDLRRIAEREALLSELEDT
                     LATRQSAVADAQAGLPGAQQALREAMEAERQTQRQATELRARREALVQLQHKVQAQGQ
                     LGDWLRRHRLDGLTPLWQRLQVAAGWESAVEAILRERLAALPAEDGRAALIALADKVP
                     GAFAFLLADAPADGAPPMALEGTRPLLDHVCADVTLTPVLAAWLGTVLAVDDLAPWLE
                     RRAELSPSVRLVAKDGRVLDRHALIHYAPDARTHGVIERQREIDQLAVELDRAQEAAA
                     QAKEAVAEADATATRLQDGLTTLRRDVQALQAQVHGEQVEVLKLNQARVRYEERWAQI
                     ERDMGEIALLEQTERDRLMRSETELERSREMAELQRGRLDAAEAVRREREAALRETRA
                     LEQALARELQDARHAERECTSKLDDIARNRSLAEEQLARTAEELEARRESLLEIDEPS
                     VAAALQAAVVLRGTREMALVEVRNGLEAIHAQVRGLEEARHHTEQTAAPVRERVAELR
                     LRVQAAELAEAQFATRLEEAQADEAALAPLLTADLKEGALQREVARLGREIAELGAVN
                     LAALDELRAASERKTYLDVQSEDLLGAIETLEDAIRRIDRETREQLQETYNTVNRHFG
                     SLFPQLFGGGEARLVLTGDEILDAGIQIVAQPPGKKNSSIQLLSGGEKALTAIALVFA
                     MFQLNPAPFCMLDEVDAPLDDANTGRYCGMVKRMSSQTQFIFISHSKITMEIAQQLVG
                     VTMQEQGVSRVVEVDIEEAMRLAETAA"
     gene            15440..>16635
                     /gene="dapC"
                     /locus_tag="PKX01_00060"
     CDS             15440..>16635
                     /gene="dapC"
                     /locus_tag="PKX01_00060"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HMZ74542.1"
                     /translation="MNPHLQDLHPYPFEKLRALFDGIVPPAEYSPIRLSIGEPQHATP
                     AFIRDALVAHLGGLANYPTTQGSDALRKSIAAWLESRYGLPAVDPATQVLPVNGSREA
                     LFAFAQCVIDGTRPDALVLCPNPFYQIYEGAAYLAGAQPYFINNLPDDNFGSDFARVP
                     DEAWARVQLVYVCSPGNPTGRVLTLDEWQHLFELSDRHGFVIAADECYSEIYFNDDAL
                     PIGGLEAAQRLGRSDFRNVVMFSSLSKRSNVPGMRSGFVAGDANILKRFLLYRTYHGC
                     AMSPVVQAASAAAWSDEAHVAENRRLYREKFDRVTPLIAEHLEVSLPDAGFYLWARVD
                     KLGLSDTEFARRLQAEYNVTVLPGSFLAREAHGTNPGAGFVRIALVAELAECLEAARR
                     IAAFAQR"
     assembly_gap    16636..16735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16736..17216
                     /locus_tag="PKX01_00065"
     CDS             <16736..17216
                     /locus_tag="PKX01_00065"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMZ74543.1"
                     /translation="GSFIGKGVVLMPSYVNIGAFVDEGTMVDTWATVGSCAQIGKNVH
                     LSGGVGIGGVLEPLQANPTIIGDNCFIGARSEVVEGVIVEDNCVISMGVYIGQSTKIY
                     DRTTGEVLYGRVPEGSVVVSGNLPSGDGKYSLYCAVIVKKVDAQTRSKTSINELLRD"
     gene            17270..18445
                     /locus_tag="PKX01_00070"
     CDS             17270..18445
                     /locus_tag="PKX01_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HMZ74544.1"
                     /translation="MIIDKLFTLMAEKKASDIYVTVGAPIHIKIDGNTLPINQQAMDA
                     GMIHRMAYEMMTPEQIARFENEREMNLSFSRRNMGNFRVNIFWQRNSIAIVVRYILDQ
                     IPRLDALGLPPVLSDVIMEKRGLVLVVGATGSGKSTTIASMLDHRNENFSGHILTIED
                     PIEYLFRHKASIVNQREIGTDTLDWNHALSNAMRQAPDCILIGEIRDRDTMQAAIAYA
                     QTGHLCLATLHANNAYHALNRIFNFFPMENRPLLYMDLAATLKCIISQRLVKRPDGKR
                     IPAVEILLNSRQVAELIERGEISGIREAMEQSLASGSRTFEQDLFRLYRDKIITLEEA
                     LANADSPTNLSWLINNAQFSDKNADKTPLPSTSIDSEPHQEEGGASFKEFTLELDED"
     gene            18467..19615
                     /gene="dapE"
                     /locus_tag="PKX01_00075"
     CDS             18467..19615
                     /gene="dapE"
                     /locus_tag="PKX01_00075"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HMZ74545.1"
                     /translation="MTRPTAPASATLALTRELIARRSVTPEDAGCLDLIASRLTPLGF
                     VCERLDAGGTCNLWARRGTARPLVVFAGHTDVVPTGPLAQWQSDPFEPTERDGFLYGR
                     GAADMKTSLAAFVTAIEDFVASHPHHPGSIALLLTSDEEGDATYGTVHVVNTLKARGE
                     TLDFCIVGEPTSVDVLGDMIKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPAL
                     TELAATRWDEGNEYFPPTSWQMSNIHAGTGTTNVIPGTLEVLFNFRFATVHTADALKQ
                     RVHAILDRHGLDYDLAWTLGGKPFLTPRGTLVGALEAAIRDALGRTPELSTTGGTSDG
                     RFIADICPQVVEFGPVNATIHKIDECIALDAVDPLSDIYRRTLEHLLA"
     gene            19627..20577
                     /gene="prmB"
                     /locus_tag="PKX01_00080"
     CDS             19627..20577
                     /gene="prmB"
                     /locus_tag="PKX01_00080"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMZ74546.1"
                     /translation="MAHEDTLPDDAGHDEHEHEHEHGPLAELVTVRDWLRYAVTRFNR
                     AGCFFGHGFVDAWDEAVYLVLHTLALPLDRLDPFLDACIPGDEREAIFDVIEKRAVDR
                     LPAAYITGEAWLGEFRFEIDPRVIIPRSYFAELLHEGFSPWIQDPASVTRAMDMCTGS
                     GCLAILMAHAFPHAEIVAVDISPDALEIAARNVAAYGLEDRITLVQSDAFDLVPEQPF
                     DFILSNPPYVTQDAMDDLPAEYRHEPALALGAGEDGLDLVRRLLADAPRFLAPDGQLA
                     VEVGHNRDLFEAAFPTLAPTWLSAPSGDEKIFLINRRQLL"
     gene            20717..21166
                     /locus_tag="PKX01_00085"
     CDS             20717..21166
                     /locus_tag="PKX01_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ74547.1"
                     /translation="MATDRPILLVEDNPDDEALTLRAFSKNKIANQVVVARDGVEALD
                     YLFCTGQYSTRDSHQMPAFVLLDLKLPRIDGLEVLRRIRADERTALLPVVVLTTSKEH
                     QDIFEAYRLGANSYIRKPVDFERFLQAVGQLGLYWLSLNEPSDPTAR"
     gene            complement(21171..21659)
                     /locus_tag="PKX01_00090"
     CDS             complement(21171..21659)
                     /locus_tag="PKX01_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ74548.1"
                     /translation="MSGGAQLHAFELPALQGGVLRLADYAGKVLLIVNTASRCGFTPQ
                     YAGLQALYETYGAQGLVVIACPCDQFGGQELGSAEEIGAFCTGRFDVRFPMADKLRVN
                     GPDAHPLFHYLTAALPGVLGSRAIKWNFTKFLVDRVGNPVRRYAPTTPPAELAGDIEA
                     LL"
     gene            complement(21682..21876)
                     /gene="iscX"
                     /locus_tag="PKX01_00095"
     CDS             complement(21682..21876)
                     /gene="iscX"
                     /locus_tag="PKX01_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HMZ74549.1"
                     /translation="MKWTDVQEIAIQLSEARPDADPARVNFVDLMSWVMALPEFDDDP
                     KRCGERILEAIQQAWIDEVA"
     gene            complement(21873..22217)
                     /gene="fdx"
                     /locus_tag="PKX01_00100"
     CDS             complement(21873..22217)
                     /gene="fdx"
                     /locus_tag="PKX01_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HMZ74550.1"
                     /translation="MTTLIVLPHVELCPEGAVIDARPGESICQSLLENGIAIEHACEQ
                     SCACTTCHVIVRDGFNTLEPAEDEEEDLLDKAWGLEPQSRLSCQALVGGTPLTVEIPR
                     YSINMVKEGKRG"
     gene            complement(22241..24109)
                     /gene="hscA"
                     /locus_tag="PKX01_00105"
     CDS             complement(22241..24109)
                     /gene="hscA"
                     /locus_tag="PKX01_00105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HMZ74551.1"
                     /translation="MALLQISEPGLSTLPHQHRLAVGIDLGTTNSLVATVRNGVPQCL
                     HDDDGRVLLPSVVQYLPDGRVAVGRGAQKAQAVDPRNTIVSVKRFMGRGLKDVAHVET
                     MPYDFVDQQGMVRLRTVQGVKSPVEVSAEILSSLRLRAERSLGGELTGAVITVPAYFD
                     DAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLENASEGVYAVYDLGGGTFDISILR
                     LSRGVFEVVATNGDAVLGGDDFDHRLFCWILDNAGISLPSDQDARRLQMKAREVKERL
                     TTDESVDIRLTLTRGDEVDLTLTREQFVEMTRHLVSKTLAPVRKALRDAGLAPEDVKG
                     VVMVGGATRMPHVQKAVGEYFGQTPLTNLDPDTVVAIGAAIQANVLAGNRAAEDDWLL
                     LDVIPLSLGIETMGGLVEKIIPRNATIPTARAQDFTTFKDGQTAMALHVVQGERELVS
                     ECRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVSARETLSGVEARVEVKPSYGL
                     SDDEITRMLQSGFEYARADMEARGLKEQQVEAARVIEATEKALMSDGDLLTADERTAL
                     DASVEALRSAAAGLDARAIKLALESFNRASNEFASRRMDRSIRAALSGHKIDDIRI"
     gene            complement(24109..24639)
                     /gene="hscB"
                     /locus_tag="PKX01_00110"
     CDS             complement(24109..24639)
                     /gene="hscB"
                     /locus_tag="PKX01_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HMZ74552.1"
                     /translation="MIDLRQDHFALFGLAPAFHIDLDQLEQAYLTLQGKVHPDRFAHL
                     SDQEKRQSMQWATHANGAFRTLRQPLTRAQYLLELRGVDPAFENNTAMAPEFLMEQME
                     WREALGEAREAADASALDDMARRIRERSRALVVQIGDEIDTLGDLPAAADTVRRLKFL
                     ERLQDEINDALASLDS"
     gene            complement(24641..24964)
                     /gene="iscA"
                     /locus_tag="PKX01_00115"
     CDS             complement(24641..24964)
                     /gene="iscA"
                     /locus_tag="PKX01_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HMZ74553.1"
                     /translation="MAVTLSEKAAQHIDNFIAKRGKGIGIRLGVRTSGCSGMAYKLEF
                     ADEVQSDDVVFESRGLKVLVDPKSLPYMDGTELDFVREGLNEGFKFNNPNVKDQCGCG
                     ESFTV"
     gene            complement(24978..25361)
                     /gene="iscU"
                     /locus_tag="PKX01_00120"
     CDS             complement(24978..25361)
                     /gene="iscU"
                     /locus_tag="PKX01_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HMZ74554.1"
                     /translation="MSYSEKVLDHYENPRNVGSFQKEDDGVGTGMVGAPACGDVMKLQ
                     IKVGKDGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQALEIKNTAIAEELALPPV
                     KIHCSILAEDAIKAAVEDYKKKHNG"
     gene            complement(25375..26586)
                     /locus_tag="PKX01_00125"
     CDS             complement(25375..26586)
                     /locus_tag="PKX01_00125"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HMZ74555.1"
                     /translation="MLKFPIYLDYSATTPVDPRVAEKMIPYLTELFGNPASRSHAFGW
                     TAEKAVEEAREQVAALVNADPKEIIWTSGATESNNLAIKGAAHFYSGKGKHLITVKTE
                     HKAVLDTVRELERQGFEATYLDVQDNGLIDLEVLKAAIRPDTVLVSVMFVNNEVGVVQ
                     PIAEIGELCREKGIVFHVDAAQATGKVDIDLARLKVDLMSFSAHKTYGPKGIGALYVR
                     RKPRVRLEAQMHGGGHERGLRSGTLATHQIVGMGEAFRIAREEMAQENARILALRNRL
                     FEGLSAIPETFVNGDLVHRVPHNLNMSFAYVEGESLIMAIKDVAVSSGSACTSASLEP
                     SYVLRALGRNDELAHSSIRFTIGRFTTVEEIDYTIDLLQRKIGKLRELSPLWEMVQEG
                     IDLNTVQWAAH"
     gene            complement(26611..27777)
                     /locus_tag="PKX01_00130"
     CDS             complement(26611..27777)
                     /locus_tag="PKX01_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMZ74556.1"
                     /translation="MFAPIYLDHNATTPLDPEVREAMLPWLGGRFGNASSRHEYGRAA
                     RMAIDEARARVAEAVNAHPTEVIFTSGGSEANNLFLKGAAARLKAGALALGAIEHPCV
                     RQPARQLRQSGWTVRELAVDAAGRIDSAAFTAVLAERPRLVSVMLANNETGVVQDVAR
                     LAAQVRAAGGWMHTDAVQAFGKLPVDFRALGVHGMSLSAHKVNGPVGAGALIVDKRVE
                     LAPLVAGGGQERDLRSGTENVLAIVGFGKACQLAAARQADRAAVLRGLQARLDAGLTG
                     LGATIFSAGAERLPNTTFFALPGIDGETLVGKLDRAGFAVASGSACSSAHPEPSPTLL
                     AMGVTPECARAAVRVSLGSGNTGQDVDGFLEAIAQIVDHLRNLTAMAVGVADAA"
     gene            complement(27794..28279)
                     /gene="iscR"
                     /locus_tag="PKX01_00135"
     CDS             complement(27794..28279)
                     /gene="iscR"
                     /locus_tag="PKX01_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HMZ74557.1"
                     /translation="MRLTTKGRFAVTAMIDLALRCADGPVTLAGIAERQRISLSYLEQ
                     LFGKLRRFELVSSVRGPGGGYTLAQPMELVTVADIIAAVDEPLDATSCGGKENCLDEH
                     RCMTHDLWTNLNRRMQEYLQSVTLASLVDAQRQKALAVSVLHDERAAIARHEAGQSLT
                     A"
     gene            complement(28384..29148)
                     /gene="cysE"
                     /locus_tag="PKX01_00140"
     CDS             complement(28384..29148)
                     /gene="cysE"
                     /locus_tag="PKX01_00140"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HMZ74558.1"
                     /translation="MFRRLREDVANVLERDPAARSRWEVLTCYPGMHAVLLHRVAHAA
                     WGRGLHWVGRFVSHLGRMLTGIEIHPGATIGRRVFIDHGMGVVIGETAEVGDDCTIYQ
                     GVTLGGTSLYRGTKRHPTLGRGVVVGAGAKVLGGFEVGDGAKIGSNAVVIKPVPAGAT
                     AVGNPARIVDAGKDESPDVKADAPARQAFTAYGVTRDLDDPLVKALHGMIDHAAETDR
                     RLRVLVERLAKAGIPVDDAVLREDGFDPQKLGKMVD"
     gene            complement(29229..29951)
                     /locus_tag="PKX01_00145"
     CDS             complement(29229..29951)
                     /locus_tag="PKX01_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ74559.1"
                     /translation="MNREGALGRVRIVLSRTSHPGNIGAAARAMKTMGLSQLVLVRPQ
                     SFPDPVATARAAGADDILAAARVVDTLEEALQGTVFSAALTARRRELAVPMCWAREAA
                     GELVCAAHGGDVALVFGNETVGLSNEEVSLCQMPVRIPTAAEYGSLNLGAAVQVLAYE
                     LRMAALEPGEPPGTAFEPATHEEIEGFYAHLDRAITASGFFNPVNSKRLWPRLRRLYG
                     RIRLERDEVNILRGMLTAFEKK"
     gene            30030..30836
                     /locus_tag="PKX01_00150"
     CDS             30030..30836
                     /locus_tag="PKX01_00150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMZ74560.1"
                     /translation="MHPTLNIAIKAARRAATVINRASLDVDLLRIETKQPNDFVTEVD
                     RAAESAIIDVLREAYPSHAILAEESGETGPAEDTEYQWIIDPLDGTTNFIHGFPQYAV
                     SIALARRGVVEQAVVFDPTRNELFTATKGGGAFLNDRRIRVSKRLRLQDALVGTGFPY
                     RQFAHVDTYLAIFKELTQKSAGLRRPGAAALDLAYVAAGRLDAFWEFGLAPWDMAAGS
                     LLISEAGGLVSDLAGESTYLTTGNVVAGTPKVFPQLLQLIQGHCEGQLTA"
     gene            30844..31464
                     /locus_tag="PKX01_00155"
     CDS             30844..31464
                     /locus_tag="PKX01_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HMZ74561.1"
                     /translation="MRAAALLRRAALAVLLLTAGAPAFAGKILVWGDSLSAGYGLRPQ
                     EAWPDLLGQRLAGEKSAWTVVNGSISGETTAGGRARLPAALDRQRPAVVIIELGANDG
                     LRGLPVPAMVANLQAMIDAARKAGAQVLLVGMRMPPNFGPDFTARYEKAFRDLARTNT
                     LRFVPFLMDGFADQPAFFQPDGLHPVASAQPRILETVWKELKPLLH"
     gene            complement(31483..32031)
                     /locus_tag="PKX01_00160"
     CDS             complement(31483..32031)
                     /locus_tag="PKX01_00160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ74562.1"
                     /translation="MMRHAASLTPSAWVTRFAPLIAAGGEVLDLACGSGRHARWLAGR
                     GYRVEAVDRDAVALELLATVPHVTTREADLEAGDWPYGDRRFDGVVVTNYLFRPRFNL
                     LLAALNPGGVLLYETFMLGNERFGKPSNPEFLLRSHELIERVAGACTILAYEQGEVSE
                     PKPAVIQRICAVKGHNPVVRLP"
     gene            complement(32028..33488)
                     /gene="gabD"
                     /locus_tag="PKX01_00165"
     CDS             complement(32028..33488)
                     /gene="gabD"
                     /locus_tag="PKX01_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HMZ74563.1"
                     /translation="MKSLKDPLLFRTQAFINGVWTDADSGARLEVCNPFDGNSLGSVP
                     AMGATETAGAIAAANAALPAWRSLLARERAAILQRWFQLIHTHADDLARMMTLEQGKP
                     LAEARGEVVYAASFVEWFAEEAKRLYGELIPTTGADRRLMVIRQPVGVCAAITPWNFP
                     AAMITRKVAPALAAGCTVIVKPAEQTPFTALALAELAHRAGFPRGVFNVLTGDPVAIG
                     GALTASEVVRKLSFTGSTEVGRLLMAQCAPTVKKLSLELGGNAPFIVFDDADLDAAVA
                     GALVSKYRNAGQTCVCANRFLVQDGIYDAFAACLAEAVKGFSVGNGLDAGVTIGPLID
                     AAAVDKVEAHVADALAQGARVLVGGRRHARGGTFYEPTVLADVTPAMRVAREETFGPV
                     APLFRFKTEAEAIHMANDTEFGLAAYFYARDVGRIFRVGEALEYGMVGINTGLISNEV
                     APFGGMKQSGVGREGSRHGIDEYLELKYLCLGGLDR"
     gene            complement(33631..35730)
                     /gene="pnp"
                     /locus_tag="PKX01_00170"
     CDS             complement(33631..35730)
                     /gene="pnp"
                     /locus_tag="PKX01_00170"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMZ74564.1"
                     /translation="MPTSIKKTFTYGSHTVTFETGEIARQAHGAVMVTMDDTVVLATV
                     VAAKNAKPGQDFFPLTVDYVEKFYAAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPL
                     FPDGFYNEVQVIVQAMSLNPEVDADIPAMLGASAALAIAGIPFAGPIGACRVGYANGE
                     YILNPTATQLKTSQMDLVVAGTETAVLMVESEARELPEDVMLGGVVFGHTQMQAAITA
                     INELVEVAGKPEWDWQPPAKDEALIARVTEIAGEKIDAAFRITAKQARSQQLSAIGDE
                     VHAALTAEGRAYDANTVNNILFDLESKVVRGRILSGEARIDGRDTRTVRPIDIRVGVL
                     PRTHGSALFTRGETQALVVTTLGTGRDEQIIDAVAGEYRERFLLHYNFPPFSTGECGR
                     FGSPKRREIGHGRLAKRALAAVLPPKEEFTYTVRVVSEITESNGSSSMASVCGGSLSM
                     MDAGVPLSAPVAGIAMGLIKDGGRFAVLTDILGDEDHLGDMDFKVAGTDKGVTALQMD
                     IKILGITKEIMQVALAQAKEGRLHILGLMKDALGEARGEVSTFAPRMLTIKINPEKIR
                     DVIGKGGAVIRALQEETGTIIEITDDGNVTISSVSAEGAQEAKKRIEDITAEVEVGKV
                     YEGPVVRILDFGAIINILPGRDGLLHVSQIANERVNAVSDYLKEGQVVRVKVLETDEK
                     GRVRLSMKALLNEQAAQ"
     gene            complement(35928..36197)
                     /gene="rpsO"
                     /locus_tag="PKX01_00175"
     CDS             complement(35928..36197)
                     /gene="rpsO"
                     /locus_tag="PKX01_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMZ74565.1"
                     /translation="MAISTEQKAQIVRDHQRAQGDTGSPEVQVALLTARINDLTGHFK
                     AHVKDHHSRRGLLQMVSQRRKLLDYLKRKNADGYRELIARLGLRK"
     gene            complement(36271..37191)
                     /gene="truB"
                     /locus_tag="PKX01_00180"
     CDS             complement(36271..37191)
                     /gene="truB"
                     /locus_tag="PKX01_00180"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMZ74566.1"
                     /translation="MVQAPPPRRVPRRIVDGVLFLDKPQGLSSNHALQQARRLLNAAK
                     GGHTGTLDPMATGLLPLTFGEATKFSQTLLDADKAYDATVLLGVTTTTADAEGEVLDT
                     RPVHVSDAQLAAALAQLRGEIEQVPPMYSALKHHGKALYEYAREGVEIPRAPRRVTIH
                     RLELLERAGDALRIRVDCSKGTYIRTLASDLGALLGCGAHLTALRRTRIGPFALDAAI
                     PLADFDACAPAARDAALAPVDALLVHMPCVSLDADQARSLTHGQAARGCAGEAGQAYR
                     VYHAGRFLGVATLAEGGLLVPTRLVSTADQ"
     gene            complement(37231..37650)
                     /gene="rbfA"
                     /locus_tag="PKX01_00185"
     CDS             complement(37231..37650)
                     /gene="rbfA"
                     /locus_tag="PKX01_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMZ74567.1"
                     /translation="MPKEFSRGQRVAEQIRRELAELIRLEVKDPRVGFITLTDVELTP
                     DYAHAKVYFTSMTGQDGLDDILRGLRRASGFLRRELGRRVRIHTLPELHFQYDPSVEN
                     GNRLSSLIDRALESDRQFHPEDEAAGDDTSGKGPSVD"
     gene            complement(37654..40473)
                     /gene="infB"
                     /locus_tag="PKX01_00190"
     CDS             complement(37654..40473)
                     /gene="infB"
                     /locus_tag="PKX01_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMZ74568.1"
                     /translation="MGQTSVTQFAGELKMPASVLLEQLQKAGVDKSRETDLLTEQDKT
                     RLLDFLRRAHGDSAAKAKITLTRKQTSEIKATDSTGRARTVQVEVRKKRVFVKRDELL
                     VPEAAAPGAALVPEMPAELPEPVLEPTPVPEPIEMAAPEPVQAAPVEVVVEVEPEPEP
                     VVEPAAPEETVAPEPEPEPVAVVPEPVAVAPAAKPEPRPQQGPVRRVVSSVISAEERA
                     SREREAQRQAELAARQAADLKEKQEREARARAAMLQRMEDEKQRQSEGDKSRQDAEAG
                     AKSGTLHKPAKADDKGARKDVKKGTPVAKEAAGDAKRRGGLKTRGDAAGGAGGWKGAR
                     GGRSGGRHHEAPAFVAPTEPIIREVHVPETISVADLAHKMAVKATEVIKTLMKMGSMV
                     TINQVLDQDTAMIVVEEMGHKALAAKLDDPDAFLEENAEHKDVVEESRAPVVTVMGHV
                     DHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVDTDRGTVTFLDTPGHEAFTAMRARG
                     AKATDLVILVVAADDGVMPQTKEAIHHAKAAGVPLVVAVNKIDKPGANPERVRQELIA
                     EGVVPEAYGGDTMFIEVSAKTGQGIDDLLEAILLQAEVLELKAPHDTMAKGLIIEARL
                     DKGRGPVATLLVQSGTLKRGDVILAGATFGRVRAMLDENGKPVEEAGPSIPVEILGLS
                     EVPGAGDEVVALADERKAREIALFRQGKFRDVKLAKQQAAKLESMFEQMGEGEVKSLP
                     LIIKADVQGSQEALAHALVKLSNEEVRVQVIHGGVGAISESDVNLAQASGAVVIGFNI
                     RADANARKLAENFGVDIRYYNIIYDAVDEVKSALSGMLAPEKREQVLGMVEIRQVFTI
                     SKVGTIAGCYVLEGLVKRNAQVRVLRNHVVIHTGELESLKRFKDDVKEVKAGYECGLQ
                     IRNFNDLVEGDQLEIYEIQEVARTL"
     gene            complement(40489..41961)
                     /gene="nusA"
                     /locus_tag="PKX01_00195"
     CDS             complement(40489..41961)
                     /gene="nusA"
                     /locus_tag="PKX01_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMZ74569.1"
                     /translation="MSREILLLVDALAREKNVSKEIVFSALESALAQATKKRIHDEAD
                     VRVSIDRETGDYESFRRWQVLPDEEVDNDEAQMGLIDAREEYPEIQVGDFVEESLEPI
                     DFGRIGAQAAKQVILQKVRDAEREQILNDFLERKEFIISGSIKRMERGNAIIEVGRLE
                     AVLPRDQMIPKENLRVGDRVKAYLLRIDRGARGPQLILSRTAPEFVIRLFELEVPEID
                     DGLLEIKACARDPGLRAKIGVKSNDPRIDPIGTCVGLRGSRVTAVRNEIGGENIDIVL
                     WSPEPAQFVIGALQPAEVSSIVVDEEAHAMDVVVDENNLAIAIGRNGQNVKLASELTG
                     WTINLMTEAESERKSEEEHSSVRQIFMSKLDVDEEVANILIDEGFSSLEEIAYVPLAE
                     MLEIESFDEETVNELRNRARNVLLTEAIVTEEQLETVSEDLLSLDGMDKPLAAKLAQA
                     GVHNRDDLADLAVDELMDMSGLDEERAKNLITRARAHWFE"
     gene            complement(41983..42411)
                     /gene="rimP"
                     /locus_tag="PKX01_00200"
     CDS             complement(41983..42411)
                     /gene="rimP"
                     /locus_tag="PKX01_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HMZ74570.1"
                     /translation="MDLQQLIEQTAEGLGYEVVDVESSPRGRLLRVFIDAPDGITVDD
                     CATVSNQLCRVFEVENVDYDRLEVSSPGLDRVLKKATDFERFAGETIQVRLNLPIDNQ
                     RNFTGTLVGLTDGVVTLETEKGSLAIPFEEINKARLVPRF"
     gene            complement(42570..43430)
                     /locus_tag="PKX01_00205"
     CDS             complement(42570..43430)
                     /locus_tag="PKX01_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ74571.1"
                     /translation="MPTSATPEREQRRPQGRDAAYTEPQRLHKVLAAAGLGSRREIEE
                     WIVAGRISVNGEPADVGQKVGPGDRVRVNGKLMPIRFQPRVPRVLLYHKPEGEIVSRD
                     DPEGRPTVFDRLPMLRKGRWIAVGRLDFNTSGLLLFVNDGDLANRLMHPRYELDREYA
                     VRLLGELDEVQKQALLDGIELDDGPARFNTLLDAGGEGVNHWYRVSLSEGRNREVRRM
                     FEALGLTVSRLMRVRYGPISLPTRLKRGMWEDMPEEDVCALAGLPKPIRAPGPRKPPR
                     STPRRTPPKG"
     gene            complement(43675..44250)
                     /gene="scpB"
                     /locus_tag="PKX01_00210"
     CDS             complement(43675..44250)
                     /gene="scpB"
                     /locus_tag="PKX01_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMZ74572.1"
                     /translation="MPPPQTPDEFKRVLETALLAAPAPLQVSELRRLFEDDPGGDLVR
                     RLLVELAGDWQGRGVELVQTASGWRFQTRPEYQIYLDRLKNERPPKYSRAVLETLAII
                     AYRQPVTRGDIEDIRGVMVSPSVLKTLESRGWVDVVGHRDTPGRPGLYATTRKFLEDL
                     GLRSLTELPPLTEIERIMDLVELPKTEEPAV"
     gene            complement(44228..44998)
                     /locus_tag="PKX01_00215"
     CDS             complement(44228..44998)
                     /locus_tag="PKX01_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HMZ74573.1"
                     /translation="MYGQPIFELPRDLYIPPEALEVFLDAFEGPLDLLLYLIRKANIN
                     VLDIPMVPLTAQYLEYVEAMRRHNLELAADYLLMAATLLEIKSRMLLPRPPRDDGEEV
                     LDPRAELVRRLLEYEQIKLAAARVDALPRAERDFEWVGVFVAEKVVERLPTVSLHDLQ
                     LAWLKIARQNRLRRHHTIQREELSVREHMSLILRKLSGDAFVVFESLFDPALGVAGLV
                     VVFLATLELVKERLVVVTQNEAFAPIYVKRSDASAADA"
     gene            complement(45159..46361)
                     /locus_tag="PKX01_00220"
     CDS             complement(45159..46361)
                     /locus_tag="PKX01_00220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMZ74574.1"
                     /translation="MFAERVLSGMRPTGRLHLGHYHGVLKNWVKLQAEYPCLFFVADW
                     HALTTDYDNPKGIENSVWDMVVDWLAAGVDPSQATIFIQSRVPEHAELHLLMSMMTPL
                     GWLERVPTYKDQQEKLVNKDLSTYGFLGYPLLMSADILIYRADKVPVGEDQLPHVEFT
                     RELARRFNHMYGREPGFEDKAREAVKKLGSKRAKLYEEFRTRFQQNGDDEGLALGKAL
                     LDEAQNLSHGDRERLYGYLEGSGKMILVEPDALLTEASRMPGLDGQKMSKSYGNTIFL
                     REEADALTKKIRTMQTDPARVRRTDPGDPEKCPVWQFHVIYSDAPTREWVDRGCKSAG
                     IGCLECKQPVVDAVLKEQEAMRERAQPYLDDPTLVRSIIADGCDKARKLAQETMRDVR
                     ESMGLSYS"
     gene            complement(46368..47027)
                     /locus_tag="PKX01_00225"
     CDS             complement(46368..47027)
                     /locus_tag="PKX01_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMZ74575.1"
                     /translation="MQELISSLAIWALPVLFAITLHEAAHGYAARHFGDRTAELAGRI
                     SLNPLRHIDPVGTLLIPGVILASSALVGGSPMLFGWAKPVPVNFGRLRHPKADMLWVA
                     AAGPLANLCMAIGWMVVLKLGFSLPENAYTLPLLKMADAGVQINAVLMLLNLLPLPPL
                     DGGRIVVSLLPAGPAWKFAQIEPYGFPILLLLLFTGILGDILDPLMALFRFALATVFG
                     L"
     gene            complement(47104..47727)
                     /locus_tag="PKX01_00230"
     CDS             complement(47104..47727)
                     /locus_tag="PKX01_00230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ74576.1"
                     /translation="MAQFFSLHPEHPQPRLIRQAAQIMREGGLVAFPTDSAYALGGHT
                     GDNELLSRIRRIRGVDERHHFTLICRDLSEIATYARVDNAQYRLLKATTPGPYTFILE
                     GTKELPRRVLHPKRKTIGLRVPDHPLVAALLAELDEPLLSSTLLLPGEEHPLTDAEEI
                     RDRLEKSVDLVIEAGPCRGEATTVIDLTSGTPELVREGRGSLAPFGL"
     gene            complement(47753..48592)
                     /locus_tag="PKX01_00235"
     CDS             complement(47753..48592)
                     /locus_tag="PKX01_00235"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HMZ74577.1"
                     /translation="MNPVNADLHCHSTASDGTLAPAQVVRRAHANGVDLLALTDHDEL
                     LGLAEAADAARDVGLRFVPGVEVSVSWLDQTVHVVGLNIDPSHPALVAGLHQVREGRD
                     GRAGQIAAELDRIGIHGALEGALRYAGNPALVSRAHFARYLVEIGIARNVHDVFLHYL
                     ARGKPGYVEHVWATLDDAVGWIRGAGGVAVIAHPGRYRLGHVEMDLLFERFRDLGGEG
                     VEVCCGAHDGGQVLAFSRVARKYGLLASRASDFHGPEDSPVDLGRAPPLPPDLTPVWT
                     RLQ"
     gene            complement(48589..49464)
                     /locus_tag="PKX01_00240"
     CDS             complement(48589..49464)
                     /locus_tag="PKX01_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ74578.1"
                     /translation="MWTSRSRFLPVRGLRYHLREWGREGAPRLFLLHGWMDVSASFQF
                     VVEALRHDWHVLAPDWRGFGHSEWRGDAYWFPDYLGDLDALLTALSPDAPVDLVGHSL
                     GGNVACLYAGVRPARVRRLVALDAFGLADTAPEQAPGRLEKWLRELSAPVAFRSYPDC
                     AALAERLRADNPRLSAERAAWLAEHLGEADGEGGIRLVGDPAHRRVNPVLYRRGEARA
                     CWQRVTAPTLWVVPEAAALRGRLGVSDEAHAEARDCFRDFREVTVADSGHNLHHDQPG
                     EVARIIETFLLSKAS"
     regulatory      49518..49609
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            49791..50873
                     /gene="gcvT"
                     /locus_tag="PKX01_00245"
     CDS             49791..50873
                     /gene="gcvT"
                     /locus_tag="PKX01_00245"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMZ74579.1"
                     /translation="MPQHTPLYATHVAAGARMVDFAGWEMPVNYGSQIEEHHAVRRTA
                     GMFDVSHMLALDLDGRDARGFLRGLIANDVAKLSEPGKALYACMLNPDGGVIDDLIVY
                     HLSDTHYRVVVNAGTADKDVAWMHERLQASGADARLTVRRDLAMIAVQGPEARAKVWA
                     AIPGAEVVSAHLKPFQAVDFGEYFIARTGYTGEDGFEITLPAEKAAGLWVDLVNAGVA
                     PCGLGARDTLRLEAGMALYGNDMDENVSPLDAGLAWTVDLKDAARDFVGKAALIAQGT
                     RRQTLGLVLEDKGVLRSHQAVFCAQGDGETTSGTFSPTLQKSIALARLPLGVQPGDAV
                     EVDIRGKRLKARVVKPPFARNGQALI"
     gene            50925..51302
                     /gene="gcvH"
                     /locus_tag="PKX01_00250"
     CDS             50925..51302
                     /gene="gcvH"
                     /locus_tag="PKX01_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMZ74580.1"
                     /translation="MNTPDDLRYTASHEWVRTEADGTLTVGITDHAQEALGDVVFLEL
                     PDAGRSVEAGEAIAVIESVKAASDIYAPVAGEIIAANQDAVDAPESVNADAYAAWLFK
                     IKPAAGASLDSLLDAAGYAATLG"
     gene            51365..54259
                     /gene="gcvP"
                     /locus_tag="PKX01_00255"
     CDS             51365..54259
                     /gene="gcvP"
                     /locus_tag="PKX01_00255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HMZ74581.1"
                     /translation="MPQNLLSSPLSALEQRDAFIDRHLGPCAVERPPMLAALGVDSVE
                     TLIAQTVPAGIRLAAPLALGEPVPEHEALAELKAIASKNVLKKSLIGMGYYDTLTPKV
                     ILRNVMENPGWYTAYTPYQAEIAQGRLEALLNFQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRIAKVKSQAFFVDEAVFPQTLDVVRTRAEYFGFELIVGPAEDAPHHEVFGA
                     LLQYPNERGEVVDPSAAIAGLKAKGAVVAVASDLMALVLLKSPGALGADIALGSAQRF
                     GVPLGFGGPHAAFFATRQAHVRAMPGRIIGVSKDARGKTALRMSLQTREQHIRREKAN
                     SNICTSQVLLANMAGMYAVYHGPQGLRTIAARIHRLAAVLAEGLRSAAYTLRTDAFFD
                     TLQLLTGARTDALYAAAQAAGFNLRHLDGHGLGISVDEKTTAADVAALLGALGAPADI
                     AALDAAVAARGGNLPTGLLRDDAILSHPVFNTHHTEHEMLRYLKRLQNRDLALDHSMI
                     SLGSCTMKLNATSEMIPVTWPEFADIHPFAPAEQTAGYRELLDGLAAQLRAITGFDAI
                     CLQPNSGAQGEYAGLVAIRRYHAANGQGQRNVCLIPRSAHGTNPATAQMCGLEVVVVD
                     CDDNGNVDLPDLEAKAARFADRLAALMITYPSTHGVFEENIREICAAVHRHGGQVYMD
                     GANLNAQVGLTSPATIGADVSHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPYMADHAV
                     APTGDEARPQAGQGAVSAAPFGSASILPISWMYITMMGGRGLRQATEIAILNANYVAT
                     LLKDHYPVLYSGGQGRVAHECILDIRPIKAATGITEVDIAKRLMDYGFHAPTMSFPVA
                     GTIMVEPTESEDKGELDRFIAAMISIRAEIHAVEDGRLPADDNPLKHAPHTQADLLGD
                     WNRPYSRSEAAFPLPWVGENKFWPSVNRIDDVYGDRNLFCACVPMDAYAD"
     gene            54444..56453
                     /locus_tag="PKX01_00260"
     CDS             54444..56453
                     /locus_tag="PKX01_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ74582.1"
                     /translation="MPPLRPPHHLMAPLARTALALGIALLGTSAQADTDALSSLKQMS
                     LDELADIRVSIASQRSERVEDTAAAVTVLTAEDIRRSGATTIPELLRTVPGLNVARIS
                     ATEWAVSARGFNNQFANKLLVMVDGRSVYTPFFSGVLWDELNLVMQDIDRIEVVRGPG
                     GSTWGANAVNGVINVITRHSEDTQGTLVGTVVGNHQSEVVARHGYRLNDHSFGRVYAK
                     VHQADRFANPDPSTQDDMRWSGGRAGFRNDWESAGHMVTVQGEAFREMKPDERQYSGA
                     HLLGRWEHGHGDGSRDELRAYYNQFSLDHWRAAGNAATAQINTADVNFRHHFADAGPH
                     SLIAGFGYRAVQADANLTLPTSVTRPQRTDQLFSIFAEDTVSLAERLRLTLGVKGEHN
                     DFTGLEWQPSARLRWTSGSGSTAWMAVSRAVRTPSIIEDSATLNSSLAPTPQTFGLPV
                     AVSAVGNPNLTSERLIAYEIGYRAQLSPRLSLDTAAFINDYDRLLSVDMIGTPSLVIP
                     PFPPRAMSWIARAGNNLEGRATGFEISLDYRPSTTWRLLTAYSYLDLNLHTKPGNTEL
                     LPLRAEGESPRHQISIISRSDLRHDLELDVQARYVSALPAQAVADYLTADMRLAWRAS
                     KSADLALVGRNLMGPAHTEFGGLALISSSAYPVPREYYLTANLRF"
     gene            56505..57071
                     /locus_tag="PKX01_00265"
     CDS             56505..57071
                     /locus_tag="PKX01_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HMZ74583.1"
                     /translation="MHALSRFPGRWRLLLTLGVATLTAPASSVEPFAEAEVKAAFLYN
                     FVHFVRWPDVARKPGDPFRFCVLDDTLAPLLSKAVQGENVDNHSLTVTRQIELRNLAE
                     CQILYVGDAVWPVHHELLRRATALRILTVSDIPSFTEKGGMIALIRKRGRIHPVINMD
                     AIARADLRVSAKLLNLATVVGDDKGGAE"
     gene            57068..59005
                     /locus_tag="PKX01_00270"
     CDS             57068..59005
                     /locus_tag="PKX01_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ74584.1"
                     /translation="MMREALLRQPIKFKIIAIALITTAIGLLVALTAQVSSEFSATIE
                     TKQEHAVSLTTILGLNSTAALSFRDPEAGREILSALASNHEIIAARLRLPDGTIFARF
                     DSTRPEHADLIQSIPTAGQAELAHREHTLTALWHNPTLEVDQAVRLNDKVIGRIEVFF
                     DLSALMKLVRERILAAAGILLAATLIAWLLASRLQRVIAAPVEALAAQMQTVTETRDY
                     SVRVAPGGTDEIGTLMNGFNAMLEQIQTRDLQLSIAKDAAEQANKAKSLFLATMSHEI
                     RTPMNGVMGMAELLADSKLDAHQQRLLGHIQNSADSLLRVINDILDFSKLEAGRMELE
                     NIPFSPKSLIEEITAFFEPKAQEKGITLKPIFRNEIPPLVLADPNRLRQILMNLVGNA
                     MKFTQTGEIVVALEAHPMRHDAPEHRQLHFSVKDTGIGIPENVMPHLFSPFTQADSSH
                     ARRYGGTGLGLAVCRKLVKLAGGEIGVESSIRHGSKFWFTVPVKSTDSITVGLSQFER
                     QQPHLTATSNATKGLRVLIADDHRINQLLATMQLEELGCHVDTAGDGKDVLNLIARKD
                     FDIILMDCQMPELDGYETTRIIRARQRNNLRTPIIAVTANALDEDRATCLEAGMDDFL
                     PKPYTQNQLKEILLKWVPDNQ"
     gene            complement(59133..59411)
                     /locus_tag="PKX01_00275"
     CDS             complement(59133..59411)
                     /locus_tag="PKX01_00275"
                     /inference="COORDINATES: protein motif:HMM:NF022446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HMZ74585.1"
                     /translation="MAVIIAAAVLANLSFISSRPFGVLPRRTLKSAWLHVSELGAAYL
                     VFLYFCLWMERAEHGVAYSQGWEFYAASLCVFLVFGFPGFVFRFFVSR"
     gene            complement(59466..60932)
                     /gene="nuoN"
                     /locus_tag="PKX01_00280"
     CDS             complement(59466..60932)
                     /gene="nuoN"
                     /locus_tag="PKX01_00280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HMZ74586.1"
                     /translation="MNFVTPDFYPAASELFVACMAIVVLMATTFAKDARSVGYWLSVL
                     TLVGAALVTLFTMDGQVSFTFSNMYVDDLMGDFLKLMLYFAVLVVLIYSRGYMNDRNI
                     ESPEFYLLVIFATLGMMVMVSANHFLTLYLGLELLSLALYAMVALQRDSVRCTEAAMK
                     YFVLGALASGLLLYGISMVYGATGTLEISGVAQAVYHQAGNRTVLMFGLVFLVAGLAF
                     KLGVVPFHMWIPDVYQGAPTSVTLLIASAPKLAAFAMAMRLLVNGLFDLADQWQSMLM
                     FMAVLSIVLGNLAAIAQTNLKRMLGYSGISHMGFMLLGLVSGVVGGDRHFALNAYSSS
                     MFYVVAYVLMSLASFGVLILMSRVGFEAETLDDFKGLNKRNSWFAGVMAISMFSMAGI
                     PFFIGFFAKFSVLQAVVAAGYIGVAILAVVMSLIGAYYYLRVVKVMFFDEPLDASVIH
                     APAEMRVMLSLNGIAIAVLGILPQGLMSVCAYALLGSL"
     gene            complement(60972..62453)
                     /locus_tag="PKX01_00285"
     CDS             complement(60972..62453)
                     /locus_tag="PKX01_00285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ74587.1"
                     /translation="MTGIPFLSLAIWVPIVGGLVALATGSDKNAPVARFVALFTALVG
                     LAVSIPLFTGFDVATSSMQFVEAYAWIPRFNINYYLGVDGISVLFVVLNSFITVIVVI
                     AGWDVIQEKVSQYMAGFLIMSGLINGIFTALDGVLFYVFFEASLIPLFIIIGVWGGPN
                     RVYAAIKFFLYTLAGSLLMLVALLYLFMESGGSFNILEWHELKLGMDVQTLIFWAFLV
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSIVLAAIGLKLGAYGFLRFSLPITPDAAQAFA
                     PMMITLSLIAVIYIGFVALVQTDMKKLVAYSSISHMGFVTLGFFIFNPLGVEGALVQM
                     ISHGFVSGAMFACIGVLYDRMHSRQIADYGGVVNVMPKFAALFMLFAMANSGLPATSG
                     FVGEFMVALGAVQHNFWVGFAAATTLILGAAYTLWMYKRVVFGAIANDHVAELKDINN
                     REFVFLLVLAVCVLAMGIYPFPVTNVMHASVSDLLKHVAAGKL"
     gene            complement(62535..64562)
                     /gene="nuoL"
                     /locus_tag="PKX01_00290"
     CDS             complement(62535..64562)
                     /gene="nuoL"
                     /locus_tag="PKX01_00290"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ74588.1"
                     /translation="MSDMQKLYLVVPLAPLLGAVIAGLFGKAIGRAGAHTVTILGVAI
                     SFVCSVFIFNDVAAGNTFNGTLYTWATSGALTFEVGFLIDTLTVMMMIVVTFVSLMVH
                     VYTIGYMTDDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWY
                     TRPTAIFANMKAFLVNRVGDFGFLLGIGLIAAYAGSLNYAEVFNKAGDLAGMTVPATG
                     WPLITAVCIGLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARM
                     SPLFELSDTALSFVLIIGATTALFMGFLGIVQNDIKRVVAYSTLSQLGYMTVALGVSA
                     YSAAVFHLMTHAFFKALLFLGAGSVIIGMHHDQDMRNMGGLWKYMPVTWITSLVGSLA
                     LIGFPFLSGFYSKDSIIEAVGVSHLAGSGYAYICVIVGVFVTAFYSFRMYFLVFHGKE
                     RFGLVHESHHDVHHHDDGDASHDEDHHHGLAPGQKPHESPWVVTVPLVMLAIPSMLVG
                     FYTIEPMLFGEYFKGVIYFSDAHPVMAELEEEFHGAVAMALHGFTTLPFFLAMSGVVL
                     AWFLYLVQPWIPEKVMLAFKPIHTLLENKYYFDKFNETVFAGGARLLGKGMWKAGDQA
                     IIDGMVVNGSAKFVGWIAQLTRLFQSGHIYQYAFSMIIGVFVLLVFWINRG"
     gene            complement(64575..64880)
                     /gene="nuoK"
                     /locus_tag="PKX01_00295"
     CDS             complement(64575..64880)
                     /gene="nuoK"
                     /locus_tag="PKX01_00295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ74589.1"
                     /translation="MLPLSHYLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLAVN
                     INFVAFSRYLGDAAGQIFVFFVLTVAAAESGIGLAILVALFRNLRTIHVDDLDTLKG"
     gene            complement(64912..65511)
                     /locus_tag="PKX01_00300"
     CDS             complement(64912..65511)
                     /locus_tag="PKX01_00300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ74590.1"
                     /translation="MDFQVVVFYFFSAIMVIASLKVITSRNPVHAALYLVLSFVSASG
                     IWLLLQAEFLAIALVMVYVGAVMVLFLFVVMMLDINIERLRQGFWSYLPVGALIAIAM
                     VVEMGMVLGTYFGLDSVPLPPAPEQGYSNTKALGRLLFTEYAYPFELASIVLLVAMIA
                     AVALTLRKRDSVKKVDPSAQVAVQRNDRLRIVSMPSDSE"
     gene            complement(65542..66030)
                     /gene="nuoI"
                     /locus_tag="PKX01_00305"
     CDS             complement(65542..66030)
                     /gene="nuoI"
                     /locus_tag="PKX01_00305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HMZ74591.1"
                     /translation="MGSMKDFVGSLFLKELLKGMALTGRHLFARKITVQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQRGDGSRRTSRYDIDLTKC
                     IFCGFCEEACPVDAVVETRIFEYHGEKRGDLYYTKQMLLAVGEKHEKQIAADREVESK
                     YR"
     gene            complement(66041..67090)
                     /gene="nuoH"
                     /locus_tag="PKX01_00310"
     CDS             complement(66041..67090)
                     /gene="nuoH"
                     /locus_tag="PKX01_00310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ74592.1"
                     /translation="MENVLQPVAEFFGDVWPLVWSLAKIVGIIAPLFLCVAYLTLAER
                     KVIGYMQVRIGPNRVGPKGLLQPIADGVKLLLKEIIVPSCASKSLFIVGPVLALGPAL
                     AAWAVVPFADGWVVGNVDAGLLFLLAITSIEVYGVIIAAWASNSKYAFLGGMRAAAQM
                     VSYEISMGFALVSVLMISSSLNLTDIVSSQGKGQWADWGFGFLSWNWLPLFPMFLVYL
                     ISGVAETNRAPFDVVEGESEIVAGHMIEYSGMAFALFFLGEYANMILISALTSILFLG
                     GWLSPFGFLPDSFVWLALKTSFILFLFLWFRATFPRFRYDHIMRLGWKVFIPVTLFWV
                     MLVAVWIMSPLSVWK"
     gene            complement(67090..69438)
                     /gene="nuoG"
                     /locus_tag="PKX01_00315"
     CDS             complement(67090..69438)
                     /gene="nuoG"
                     /locus_tag="PKX01_00315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HMZ74593.1"
                     /translation="MLEIEIDGKQLQVPDGSTVIEAASKVGAYVPHFCYHKKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTNGMKVWTHSEPAIKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLSVGYGGVSSRYQEEKRVVSNKDLGSLVSTDMTRCINCTRCVRFTTEIA
                     GLMELGQAFRGEHAEIMPFVEKALESELSGNIIDLCPVGALTSKPFRFGARAWEMSRR
                     KSVSPHDSLGSNLIVQIKNDKVKRVLPLENEAINECWLSDRDRFSYEAVNGPERLLKP
                     MIKQGGVWQEATWEVALEYVVNGLKSVSKEHGPKSIGAWISPHSTLEEMYLAQRLMRG
                     LESGNVDFRLRQSDFSADDKLEGAPWLGMSIADVSKLDRLLIVGSFFRKDHPLLAQRV
                     RQAAKKGLKINALGAAFDDWRLPLANVCKVAPDEFKVVLARIAGRIASQTSQSISDEL
                     ASFVSESGADNESSAIADSIASGRKIAIWLGNQAAQDVESVQLQILIQEIARLSRASF
                     GVIGEAANSVGGYIAGAIPHADLAGMNVQQMINGDLKACVLLGVEPEYDMADGVAART
                     ALQNAETVIVLNAFKSKDALNYADVMLPVAPFTETAGTYVNAEGAIQSFYSVVKSTGD
                     SRPAWKILRVLGSMAGIPGFEFERIEDVRDASLGPNHANVKLSNVLKGCVVTAKRKAC
                     SLQRVSDIPPYAADPIVRRSEPLQNTLDAVIPTARMSSKTLAEIGVGDGEKVRVKSKS
                     GEVTIFAQVDSGVADGCVRLAGAHDATKLLGSLSAEILVERA"
     gene            complement(69432..70751)
                     /gene="nuoF"
                     /locus_tag="PKX01_00320"
     CDS             complement(69432..70751)
                     /gene="nuoF"
                     /locus_tag="PKX01_00320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ74594.1"
                     /translation="MSAQGIILSGLDGDRTWRLTDYLKRGGYEALKKIIADKIPADQI
                     IAEVKKSALRGRGGAGFPTGLKWSFMPRAFPGAKYIACNSDEGEPGTFKDRDILRFNP
                     HSVIEGMVIAGYAMGCERGYNYIHGEIFETYERFEEALQEARDAGLLGENILGSDFSF
                     ELFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASI
                     PYIIREGGDAFLALGKPNNGGTKLFSVSGHVNRPGNYEIPLGTPFPVLLEMAGGMRGG
                     RKLKGVIPGGSSAPVLPGHVMMDCTMDYDSISKAGSMLGSGAVIVMDESTCMVKSLER
                     LSYFYFEESCGQCTPCREGTGWLYRVVHRIENGLGRPDDLDLLNSVTANIMGRTICAL
                     GDAASMPVQSFIKHFGDEFAYHIEHKKCLVPVDVQREGSKIFVGSTC"
     gene            complement(70748..71224)
                     /gene="nuoE"
                     /locus_tag="PKX01_00325"
     CDS             complement(70748..71224)
                     /gene="nuoE"
                     /locus_tag="PKX01_00325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMZ74595.1"
                     /translation="MLSPESLKKIDREIAKYPVDQKQSACMSALRIAQMEKGWLAKET
                     IEFVARYLDMPSIAAYEVATFYNMYDLTPVGAKKITICTNLPCVLSGGVHAAEYLKKK
                     LGIDFNETTADGLFTLKEGECMGACGDAPVLLLNNHRMCSWMTEEKIDQLLADLKK"
     gene            complement(71287..72540)
                     /locus_tag="PKX01_00330"
     CDS             complement(71287..72540)
                     /locus_tag="PKX01_00330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ74596.1"
                     /translation="MAEIRNYTINFGPQHPSAHGVLRLVLELDGEVVERADPHIGLLH
                     RGTEKLAETRTWIQSVPYMDRLDYVSMMCNEHAYCMAIERLLGLEVPLRAQYIRVMYD
                     EITRILNHLLAVGTHALDIGAMTMVLYTFREREDLMDAYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPQYEVSKWKSEKIVRELNENRQGSLLDFLEDFCERFPRYVDEYETLLTDNR
                     IWKQRTVGIGVVSPEQALALGFTGVMLRGSGIEWDLRKKQPYEVYSQMDFDIPIGTAG
                     DCYDRYLCRIEEMRQSNRIVKQCIEWLRKNPGPVISDNHKVAPPAREQMKANMEELIH
                     HFKLFTEGIHVPAGEVYAPVEHPKGEFGVYAISDGANKPYRLKLRSPGFAHLSAMDAM
                     SRGHMIADVVAIIGTMDIVFGDVDR"
     gene            complement(72533..73138)
                     /locus_tag="PKX01_00335"
     CDS             complement(72533..73138)
                     /locus_tag="PKX01_00335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMZ74597.1"
                     /translation="MSSKLENLTRCLSEKFADDIVRLIDAAGEVTLEVRSENYLAVAE
                     SLRDDPELAFEQLVDLTGVDYSVHALADSFSGRFAVVSHLLSIRHNVRLRLRTFAEDS
                     DFPMVASLCAVWPSANWFEREAFDLFGVMFMGHPDLRRILTDYGFVGHPFRKDFPVSG
                     FVEMRYDQDLGRVIYQPVSIEPRENTPRIVREDSYGDVGHG"
     gene            complement(73151..73627)
                     /locus_tag="PKX01_00340"
     CDS             complement(73151..73627)
                     /locus_tag="PKX01_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HMZ74598.1"
                     /translation="MSIEGVFKEGFVTTSLDTVINWTRTGSLWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIVQLQNKIKRECTIAR"
     gene            complement(73691..74065)
                     /locus_tag="PKX01_00345"
     CDS             complement(73691..74065)
                     /locus_tag="PKX01_00345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMZ74599.1"
                     /translation="MLEAYFPVLVFILFAAGFGLIPILAGWILGPSKPDAEKLSPFEC
                     GFEPFEDARIKFDVRYYLLAILFILFDLEVAFLFPWATILREIAGTEAVRSFGFFEML
                     VFLGILLVGYIYVWKKGALDWE"
     gene            complement(74158..74242)
                     /locus_tag="PKX01_00350"
     tRNA            complement(74158..74242)
                     /locus_tag="PKX01_00350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74206..74208),aa:Leu,seq:gag)
     gene            complement(74305..74670)
                     /gene="secG"
                     /locus_tag="PKX01_00355"
     CDS             complement(74305..74670)
                     /gene="secG"
                     /locus_tag="PKX01_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMZ74600.1"
                     /translation="MGNMMFSLVLVVHVLAGLGVIGLVLVQHGKGADMGAAFGSGASG
                     SLFGSSGSANFLSRTTGVLAAVFFVTSLGLSYLAGGAERKQPTSVMEGVVHPPVSGGV
                     ATPPPPPIEDASKAKDIPR"
     gene            complement(74663..75442)
                     /gene="tpiA"
                     /locus_tag="PKX01_00360"
     CDS             complement(74663..75442)
                     /gene="tpiA"
                     /locus_tag="PKX01_00360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ74601.1"
                     /translation="MRKKLVVGNWKMNGGLGQNASLLSSLRQAEALAGVNVAVCVPFP
                     YLSQAQQMLSQSSVAWGAQDLNMHDSGAFTGEVSGGMLRDFSCSWVIVGHSERRALFG
                     EGDDVVGRKVRAALRVGLSPILCVGEALAERRSGVAESVVKRQLSVLAGLSADELERV
                     VVAYEPIWAIGTGVTASPEEVRSMHRLIRQWLGSMHGEHVVDKLRILYGGSVTAENAA
                     LLFSQDDVDGGLVGGASLKFDSFFAVCQAASVGTGSGEICG"
     gene            complement(75558..76913)
                     /gene="glmM"
                     /locus_tag="PKX01_00365"
     CDS             complement(75558..76913)
                     /gene="glmM"
                     /locus_tag="PKX01_00365"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ74602.1"
                     /translation="MSRRYFGTDGVRGRVGELPITPDFGLRLGYAAGKTLVAHENLRP
                     GEHPAVIIGKDTRISGYMLESALEAGFSAAGVDVYLAGPLPTPAIAYLTRALRLQVGV
                     VISASHNPYDDNGIKFFSASGTKLPDAVEQEIEARLDEPMGCESSAALGKARRIDDAR
                     GRYVEFCKSTFPNELDLRGMKIAVDCAHGAAYMVAPKVFHELGAEVIATGVEPNGLNI
                     NEGVGATSPTHLRDTVLASGADIGIALDGDGDRIMMADSAGNLYDGDKLLYVIAKAAL
                     VAGSPGAGVVGTLMSNLGFEHAIARLGLPFARAKVGDRYVLEQMHERRWRLGGENSGH
                     IICLDKHTTGDGIVAGLQVLAALRKQGLTLEQMCEDLEFYPQKLVNVPLRSGFVWTDS
                     REIARAVDDAKATLGQSGRVLLRPSGTEPLLRVMVEGQDARIVEQLAHGIAEVVRAAA
                     M"
     gene            complement(76922..77728)
                     /gene="folP"
                     /locus_tag="PKX01_00370"
     CDS             complement(76922..77728)
                     /gene="folP"
                     /locus_tag="PKX01_00370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ74603.1"
                     /translation="MAIVNVTEDSFSGDGCKAFDHVLRAAERAVEQGADLIDVGGESS
                     RPGAAGVSEAEELERVVPVIAALQGLGVPLSVDTVKPRVMKAALDAGADLINDIAAFR
                     QPGALEAVKDSSAALCVMHMQGEPRTMQIEPVYIDVVDEVRIFLDERVKVLLQAGVAA
                     ERILVDPGFGFGKTLEHNLALLARLEVFGKSGFPVLVGMSRKSMLGALTGRPVGERQV
                     AGAVAAMWAIQRGARIVRTHDVAATRDVIRVFSGLEAAEFRMRRHCEPSD"
     gene            complement(77850..79739)
                     /gene="ftsH"
                     /locus_tag="PKX01_00375"
     CDS             complement(77850..79739)
                     /gene="ftsH"
                     /locus_tag="PKX01_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMZ74604.1"
                     /translation="MNNLFKNLAIWLVIGVVLMTVFNQFNNRQTAQNSVEYSQFMEDA
                     KQGKIGKAVVDGRTVRATTQDGRVVTVYTPGVQDIWMVGDLMRYGVKVTASKPEEEQS
                     FLMNIFVSWFPMILLIGVWVFFMRQMQGGGRGGAFSFGKSKAKMLDESANTVTFADVA
                     GCDEAKEEVGELVDFLRDPSKFQKLGGHIPKGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FSISGSDFVEMFVGVGAARVRDMFEQAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDER
                     EQTLNQLLVEMDGFEGVSGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQ
                     ILRVHMRKVPIAPDVDALVLARGCPGFAGADLANLVNEAALFAARGSKRLVDMDDFER
                     AKDKIMMGAERRSMVMPEEERRNTAYHESGHAVVAKCLTKTDPVHKVTIIPRGRALGV
                     TMQLPEQDRYSQDRERLLQTIAVLFGGRIAEEIFMNQMTTGASNDFQRATDLARRMVT
                     QWGMSDNLGPMVYGEEEGEVFLGRSVTTHKNMSEATLQKVDAEIRRILDQQYALARRL
                     IEENREKVEAMTTALLEMETIDSDQINDIMDGRTPRPPKPSQVPRRPSDDAPGAAPTA
                     PATAA"
     gene            complement(79856..80476)
                     /gene="rlmE"
                     /locus_tag="PKX01_00380"
     CDS             complement(79856..80476)
                     /gene="rlmE"
                     /locus_tag="PKX01_00380"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HMZ74605.1"
                     /translation="MKRSKTSKSWMMEHVNDPFVQRAKTEGMRARAAYKLMEIDEKDK
                     LFRSGMTVVDLGAAPGSWSQVAVGKVGGAGRVFALDLLPMEPIPGVHFMQGDFHDDAF
                     VEAFEVQLGDGQVDLVLSDIAPNISGIPSSDQARSIHLAELALDFAARHLAANGRFLV
                     KVFQGEGFDDYRKAMGEAFRSVVVRKPQASRGRSAEVYLLGTGKRS"
     gene            80513..80965
                     /locus_tag="PKX01_00385"
     CDS             80513..80965
                     /locus_tag="PKX01_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HMZ74606.1"
                     /translation="MMIELTPAQRRAFRAQAHHLHPVVSVASNGLTPAVLNEVDAALQ
                     AHELIKIRVYGEDRAQRDAILATVCVELGAAAVQHIGKTLVVWRERREDEKTVAPVTD
                     KPRRSLAKAKSAKVLSARRGVSSLKPKSAQKKPYWFASPSDRKPRRED"
     gene            complement(80986..81426)
                     /locus_tag="PKX01_00390"
     CDS             complement(80986..81426)
                     /locus_tag="PKX01_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HMZ74607.1"
                     /translation="MYSLLITLWVGALWTVGYMVAPTLFHMLDDRALAGRLAGQLFTY
                     VGWLGMASAAYLLVFVALRKNGAAFKSSVFWLVLVMLLLTVAGAFGIQPVLAQLKAES
                     LPREVMEGAVRDRFVAWHGVSSVVYLVESVLGVLLVVVQGRGLR"
     gene            complement(81479..81955)
                     /gene="greA"
                     /locus_tag="PKX01_00395"
     CDS             complement(81479..81955)
                     /gene="greA"
                     /locus_tag="PKX01_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMZ74608.1"
                     /translation="MSKIPLTVAGAEKLRQELHRLKTVDRPNVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFIEGRIREVEGKLGNAQIIDPTLLDADGRCVFGATVDLEDLDSGAK
                     VSYQIVGDDEADIKEGKIAISSPIARALIGKFAGDVAEVQAPGGVRELELIDVRYV"
     gene            complement(81952..85176)
                     /gene="carB"
                     /locus_tag="PKX01_00400"
     CDS             complement(81952..85176)
                     /gene="carB"
                     /locus_tag="PKX01_00400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMZ74609.1"
                     /translation="MPKRTDIQTILIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPGTADVTYIEPITWQVVERIIEKERPDAVLPTMGGQTALNCALDL
                     ARHGVLEKYGVELIGAKEEAIDKAEDRQKFKAAMTRIGLGSARSGIAHALEEAHQVQA
                     EIGFPVIIRPSFTLGGTGGGIAYNTEEFVEICKRGLEASPTNELLIEESLIGWKEYEM
                     EVVRDKADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQILRNASIAVLREIG
                     VDTGGSNVQFAINPKDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LKNDITGGATPASFEPSIDYVVTKIPRFAFEKFPLANDRLTTQMKSVGEVMAMGRTFQ
                     ESFQKALRGLETGAYGLDEVEADQDDLERELGDPGAQRIWYVGQAFREGMSLDQVHAL
                     TRIDPWFLVQIEDIVKTEKTLAGRSLKGISAAELRELKRKGFSDRRLAKLLATDETSV
                     RLHRHTAGVRPVFKRVDTCAAEFATSTAYLYSTYEEECEAQPTGRKKIMVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDILEIV
                     AVEKPVGVIVQFGGQTPLKRARELEANGVPIIGTSPDMIDAAEDRERFQKLLFELGLK
                     QPPNRTARTPEDAVRLAAEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMREAVKVSN
                     DSPVLLDRFLNDATEVDVDALSDGHRVIIGGIMEHIEQAGVHSGDSACSLPPYTLSAK
                     LQDELRRQTEAMARALNVVGLMNVQFAIQGEGDDAVVYVLEVNPRASRTVPFVSKACS
                     LPLAKIAARCMAGRSLDDQGVSGEVVPPYYAVKEAVFPFVKFPGVDTILGPEMKSTGE
                     VMGVGRTFAEAFVKSQLGAGVRLPTAGKAFISVKHTDRPKAVEVARELVDLGFSVVAT
                     RGTAGAIAAAGLPVSAVNKVNEGRPHVVDMIKNNEIALVINTVEEKRQAVTDSRSIRT
                     SALAAKVTVYTTIEGARAACMGMRHLSGLEAYSLQALHAELSQA"
     gene            complement(85243..86391)
                     /gene="carA"
                     /locus_tag="PKX01_00405"
     CDS             complement(85243..86391)
                     /gene="carA"
                     /locus_tag="PKX01_00405"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMZ74610.1"
                     /translation="MSAFPPAILALADGTVFYGKGIGAVGMTTGEVVFNTSMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGINQEDCEAGRVYAAGLVIRDLPLLASNWRNEQSLDA
                     YLKAENIVAIAGIDTRKLTRILREKGAQGGCIVTAATTAELSSEAALAEARAFPGLAG
                     MDLAKVVSAAESYAWTEGEWQLDKGYVPQEAPRFHVVAFDYGVKRNILRMLAERGCRL
                     TVVPAQTPAAEVLALNPDGVFLSNGPGDPEPCDYAIAAIREVLAKGIPTFGICLGHQL
                     LALASGARTLKMKFGHHGANHPVKDLDSGQVLITSQNHGFAVDVATLPANLRATHVSL
                     FDGSLQGLARTDVPAFCFQGHPEASPGPHDVAYLFDRFIALMEGRRAS"
     gene            complement(86562..89165)
                     /locus_tag="PKX01_00410"
     CDS             complement(86562..89165)
                     /locus_tag="PKX01_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ74611.1"
                     /translation="MNLPSLPPPASSDELVLALRVLLQDLASRGCITMPSAASEANPA
                     ELVDGVRALIAAKQRIEHQYHETRERLDLAMQNTDAGLWDWDLAHDTIVLGEHWRQIL
                     GYAPGEGSADFDGWLHRVHPDDLQVMRHQLAAHLRGDLEDFDAQFRIATNATAWRWIQ
                     VRGRASNRNAAGRWCRLVGTFRDITRVKEAELELLDAKEMAEAANRTKSDFLANMSHE
                     IRTPMNGIIGMTDLVLDTDLDPVQRDYLLAVKNSADTLLHIINDVLDFSKIEAGRMSL
                     EQIDFSVVELMGATMKSLALTAYQKGIECLYRVAPAVPAVVVGDPTRVRQVLTNLVGN
                     AIKFTERGEVVVSVDGESLDGNEFLLRVAVRDTGIGIPADKQEQVFGAFSQADESTTR
                     KYGGTGLGLAISRRIVQAMGGDLSLESEEGRGCVFSFTVRLGRGVQANGRPPPVFAGR
                     RALVAAANGAVRELLRAELERAGVTVREAADGEALAAQLKSAQDTAKPFDWLIMEAAM
                     SAPGGFALAEQLRKGWPTLDRLVVVSDPKSQRSDADRCEKLRIGARLIKPFSAGDLSE
                     ALSLALHVDRLEQVDYLEFQGEQTAWESAPRIAERLAVLLVEDNPVNQVVAVKLLEKA
                     GHTVTVAVNGREALAQYDKRRFDVIFMDIQMPEMGGIEATQAIRAREARRSWAAGRQS
                     LSVPIIAMTAHAMQGDKEACLMAGMDDFVTKPIRPPELYAAIARVRQAQSFAASDEHL
                     ADAGLLNGDAAIEPEEIADLGATRDLLDGDEDQLQNLIRIFFNDFDRNRKALDAALAT
                     RDFARLRDVAHTLKGSAGVFSASRVVASAAQVEKAARAADEAECRRCVNELMRELGVL
                     AGALRRARRGG"
     gene            complement(89296..90102)
                     /gene="dapB"
                     /locus_tag="PKX01_00415"
     CDS             complement(89296..90102)
                     /gene="dapB"
                     /locus_tag="PKX01_00415"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMZ74612.1"
                     /translation="MSQVRIAIAGASGRMGRMLIETTLKAADMKLVAAFEQLGSPFVG
                     KDAGELVGQACGVAISCDVAAGLAEADCLIDFTRPEGTLHHLALARAARCAMVIGTTG
                     FGPEGKAAIGAAAQDIPVVFAPNMAVGVNAVFKLLDVAARILNEGYDVEIIEAHHRLK
                     VDAPSGTALRMGEVVASALGRNLEECAIYGREGVTGERDSQTIGFATIRGGDVVGDHT
                     VLFAGIGERIEITHKSSSRMSYATGSLRAARFLAGKDSGLFDMQDVLGLR"
     gene            complement(90151..90507)
                     /locus_tag="PKX01_00420"
     CDS             complement(90151..90507)
                     /locus_tag="PKX01_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HMZ74613.1"
                     /translation="MRPLLCLLLVLPTLGGCSAVIDFLKPYRIDVRQGNFVTQESVSQ
                     LKPGMSRDQVRFLLGTPLLNDVFHTDRWDYVYRLQPGKGEAQERRLTVVFEDGKLVRL
                     EGDVRAAGGEGTATRP"
     gene            90619..91071
                     /gene="fur"
                     /locus_tag="PKX01_00425"
     CDS             90619..91071
                     /gene="fur"
                     /locus_tag="PKX01_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HMZ74614.1"
                     /translation="MSSNSQNLKNIGLKATTPRLKILDLFQKADNRHLTAEDVYRLLI
                     AEDMDIGLATVYRVLTQFEQAGLLERHYFESGKAVFELNEGHHHDHLVCLQCGKVEEF
                     YDPEIEERQNRIARERGYEVHEHALYLYVDCKKTDCPHRPGKSGTPLI"
     gene            91101..91679
                     /locus_tag="PKX01_00430"
     CDS             91101..91679
                     /locus_tag="PKX01_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HMZ74615.1"
                     /translation="MEAFLTSAALVALAEIGDKTQLLSFVLAARLRKPWAIVAGIFVA
                     TILNHALAGSVGVWLANLIPAHWLPWIVGSVFVVFGLWTLKPDTLDDDEAPAAMHPAG
                     AFMTTLIAFFMAEMGDKTQLATVALAARFDALGWVVLGTTIGMMIANVPAVLLGEALA
                     RRLPLTQIRWAAAAVFILTGLVTFAGAAGLAG"
     gene            complement(91700..93370)
                     /gene="recN"
                     /locus_tag="PKX01_00435"
     CDS             complement(91700..93370)
                     /gene="recN"
                     /locus_tag="PKX01_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMZ74616.1"
                     /translation="MLRHLTVRDFVIVDHLELEFCAGFGALTGETGAGKSILLDALGL
                     ALGGKGDAGVVRSGQARAEIVAEFDLPADSALAAWLEEQSLTSEDGTVQMRRVIDSSG
                     RSKAWLNGSPVVLGQLRAAGELLADIHGQHAHHALLRSEAQLALVDAHGGLSADAVEV
                     ARRFRVWMGARKAREASEAGAASSERERELLTWQVTELRELAFEPDEWDALNQEQARL
                     AHAASLIEGSGEILDGLAEGELALVSLLERLAGRAADMRQYDARVGDVEDLLRGAGIQ
                     LDEAVHALRRYRDRLDLDPQRLTEVDQRIQAVTALARKHRCAPADLPALLAESDARLA
                     QLTRQSDPEALAREEAAAQEAFLAVARPLSVARKQVASALSAAVTEAMQTLAMAGARF
                     EVCLATEVAGAATGLERVEFLVTANASQPLRPLAKVASGGELSRIGLAIQVITSRNAA
                     TPTLLFDEVDVGIGGRVAEIVGRLLRQLGSDRQVLCVTHLPQVAAQANWQWSIAKDND
                     GDETLSRVMVLTPTERIEEIARMLGGVQITDTTRSHAAEMLAAAGNPV"
     gene            complement(93390..94274)
                     /locus_tag="PKX01_00440"
     CDS             complement(93390..94274)
                     /locus_tag="PKX01_00440"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMZ74617.1"
                     /translation="MAHAFDTIALIGKYQSPEVAEAVLRLANYLRGLGLPVIIEQGTA
                     SATGLTSGFTVGSYEEIGVRADLAVVLGGDGTLLHSACRLAGYDVPLVGVNQGRLGFL
                     TDISRDVALDKIGEILAGRFTEEHRVLLDVEVLRGGQRVFRTTVLNDVVVNKGELGRM
                     IEFDLRVDGEFVYTQRSDGMIVSTPTGSTAYALSANGPILHPSVDGIALVPMCPHALT
                     ARPITLPDRCCIEIVLLPPHDSRVHFDGQSRYDARAGDTVRITRSPHTVRLLHPVGYS
                     YFAMLREKLHWSSTPRHS"
     gene            94464..95492
                     /gene="hrcA"
                     /locus_tag="PKX01_00445"
     CDS             94464..95492
                     /gene="hrcA"
                     /locus_tag="PKX01_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HMZ74618.1"
                     /translation="MSSLDQRSQILLKTLVEHYIADGQPVGSRALSRFSGLQLSPATV
                     RNVMSDLEELGFITSPHTSAGRIPTARGYRFFVDSLLKVSPVGSGQIETLRDQIQPGE
                     PQRVINAASQLLSDLTHFAGVVISPRQESTRIRQIEFLRLSEGRILLIVVTATGDVQN
                     RILFTKRSYSPSELVTAANYLNQHFAGLSFDEIRIRIQDELKQLRSDMTELMTAAVEA
                     GSAAVAEHQQTYVISGETNLLDAEDLSSNMARLRELFKLFEQKTGLMQLLDISNRAQG
                     VQIFIGSEAGLSPLDDCSVVTAPYEVDGQLVGSVGVIGPTRMAYERVIPIVDITARLL
                     TNALSGQN"
     gene            95502..96599
                     /gene="hemH"
                     /locus_tag="PKX01_00450"
     CDS             95502..96599
                     /gene="hemH"
                     /locus_tag="PKX01_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HMZ74619.1"
                     /translation="MTRFWQEPPFRHGSPSRTGILIVNLGTPAAPTADALRPYLREFL
                     SDPRVVEIPRPIWWLILNGIILNTRPAKSARKYASVWTDEGSPLKVHTERQAKLLAGF
                     FVADGAPDLVVNWAMRYGQPSVRSRLDEMRAQGCTRVLVIPMYPQYAASTTASVLDAV
                     GDCLQHWRNLPEIRYVRSFADDPGYIGALAASVREHWQRNGQAEKLVLSFHGIPRRSL
                     DLGDPYHCECHKTARLLGEALKLPAERLVISFQSRFGRAKWLEPYTQATLEALARDGV
                     TSVDVMCPGFTADCLETLEEINMECRDAFLAAGGKAFGYIPCLNERDDWIRALRTIAR
                     RHLGHWLDLPAEDPARLAASAERARQLGATI"
     gene            96633..96974
                     /locus_tag="PKX01_00455"
     CDS             96633..96974
                     /locus_tag="PKX01_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMZ74620.1"
                     /translation="MKLILRWFLNAVALMLIPEVVTGIHVDSYSAALISAVLLGFVNA
                     ILRPLLLLITLPITVLTLGLFALVINGLMFWLVSGLVQGFTVTDFGTAFLGALLYSLL
                     TWLVNLALGGD"
     gene            complement(96999..97505)
                     /locus_tag="PKX01_00460"
     CDS             complement(96999..97505)
                     /locus_tag="PKX01_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HMZ74621.1"
                     /translation="MDNDTSRTHGWLVTIQRRGVIYRKHFSDGVFGGKQRSFSAAKEY
                     RDQIIAAHPPLSMQEYSNIVKKNNRSGVVGVCRYCASETRDLPEEKQRWFWVASWPLP
                     DGRRKRVKFSVKKYGEDGAFRMAMDARAMALEKLEGAFDPGAVRRKSSRRRTTPVPAT
                     GGAPASRN"
     gene            97670..98104
                     /gene="trxC"
                     /locus_tag="PKX01_00465"
     CDS             97670..98104
                     /gene="trxC"
                     /locus_tag="PKX01_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HMZ74622.1"
                     /translation="MPDPILVPCPHCGAINRLPGERLRDQGRCGRCKQALFTGEPLHL
                     TGEDFWIHAGRGEIPLVVDFWAPWCGPCRSMEASFAAAARRLEPAYRLGKVNTEDEPD
                     LATRFGIRSIPTLVLMRGGREVARRSGAIGADELVRWLTSVS"
     gene            complement(98136..99497)
                     /locus_tag="PKX01_00470"
     CDS             complement(98136..99497)
                     /locus_tag="PKX01_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ74623.1"
                     /translation="MDKAPDKVGKYKLLREIGRGATATVYLAEHPQYPDPVALKLIRF
                     DDKSKDEAKWNRRLLKLLRAEMDVSRRLDHPNIIKIFDTAVDKDFAYIVMEYFPGTTL
                     EHYCSFERLLPLHRTIGMVFKCCMALDYAYHQGIVHRDIKPANILVDENDNVKITDFG
                     LALNISKKSASDSTFIMGVGSPAYMSPEQIKGYPLNQKTDLFSLGVVLFHMLTGRLPF
                     RAKNPAQLIYKIINAEPPLVSQINPEIPEQMDAVIRKSLEKDLYSRYKNGADFAKDLS
                     AVRYKIFDDSYVPPDNRRFSQLRKMSFFLEFDDVEIWEVLRISRWRRVDSNTLLMREG
                     DADQRFGIIVDGKVELSVDGHRVCELGDGELFGELAFLDQQDHKHMYTVVSTTSLTYL
                     EMNPAALSLATEECQDAVRSKVVTVAARRLGNSMRELSKDGPPAERITASGFGMELQL
                     VDS"
     gene            99653..100237
                     /gene="grpE"
                     /locus_tag="PKX01_00475"
     CDS             99653..100237
                     /gene="grpE"
                     /locus_tag="PKX01_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMZ74624.1"
                     /translation="MQDQTTPPTPDHAPELVQGSDLPPAAPDAPEAPVDVVPGLEQAL
                     RQAELKAAEHHDAWLRARAETENVRRRAQDDIAKAGKFAAEKFAQAMLPVKDSLEAAL
                     ASENATLESLRQGVELTLKQLLSAFQGASVTELNPLGEKFDPNRHQAISAIEADGEPN
                     TVINVLQKGYLLHDRVLRPALVVVSKARSAQPAA"
     gene            100333..>100823
                     /locus_tag="PKX01_00480"
     CDS             100333..>100823
                     /locus_tag="PKX01_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMZ74625.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEAGKPKVIENSEGARTTPSIVAYAD
                     DGEILVGAPAKRQAVTNARNTLYAVKRLIGRRFEEKEVQKDIDLMPFTIAKADNGDAW
                     VEVRGKKIAPPQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGL"
BASE COUNT        19477 a        34133 c        29506 g        17607 t
ORIGIN      
        1 cccgcttctt ccccgcctgg ggccaggccc gcctgcacgc catgatcgcc aaccgccccg
       61 actggtgcat ctcgcgccag cgcaactggg gcgtgcccat ccccttcttc ctccacaagg
      121 ccaccggcga actccatccg cgcaccgtcg aactgatgga agctgtcgcc acgcgggtcg
      181 agcaggaagg catcgaagcc tggttcaagc tcgaccccgc cgaactcctc ggcgacgagg
      241 ccgcccagta cgacaagatc agcgacaccc tggacgtgtg gttcgattcc ggcaccaccc
      301 actggcacgt gctgcgcggc tcccatccgg acggccacgc caccggcccc cgcgccgacc
      361 tctacctgga aggctccgac cagcaccggg gctggtttca ctcatcgctg ctcaccggct
      421 gcgccatcga cggccatccg ccctatcacg ccctgctgac ccacggtttc gcggtggacg
      481 gcagcggccg caagatgagc aaatcccttg gcaacgtggt cgtgccccag gaagtcaccg
      541 gcaaactggg cgccgagatc ctgcgcctgt gggtggcgtc caccgactac tccggcgagc
      601 tgtccatctc caaggagatc ctcgaccggg tcgtcgaggt ctatcgccgc ctgcgcaaca
      661 ccctgcgctt cctcctcgcc aacaccgccg acttcaaccc ggccaccgac atgctgccgg
      721 tggaccaatg gctcgagatc gaccgctacg ccctggccct cacccgccgg ctgcagcagc
      781 aggcggaggc cgactatgcc cgctttgaat tccaccgcgt cgtgcaggcc ctgcagaact
      841 tcgccgccga agacctgggc gccgtgtacc tggacatcct gaaggaccgc ctatatacca
      901 ccgccgccac gtccgcggcg cgacgctcgg cccagagcgc cctgtggcac atcctgcagg
      961 cggtggtcaa actcatggcc ccgatcctct ccttcacggc cgaggagatc tggctgctgc
     1021 tgggcaaaga tgccgacgac agcgtgatgc tccacacctt ccacaccctg cccacgcagg
     1081 acggagagga cgccctgctg gcccgctggg aggcgatccg cgccgtacgc gccgaggtca
     1141 tgaagcagat cgaggccgtg cgcagcgacg gcaagctagg ctcgtccctg caggccgagg
     1201 tggagctgcg cgccgcgggc gcccagtacg acctgctcgc cagcctgggc gacgacctgc
     1261 gcttcgtgct gatctgctcg aagaccgtcc tcacccgcgt agacagcgag gccgacgcgg
     1321 ccatcgtggt cagtccctcg gcccagcgca agtgcgaacg ctgctggcac taccgcgacg
     1381 atgtgggtca tgatcccgct catccggaac tttgcggccg ctgcacgtcc aatctgcacg
     1441 gcaccggcga atcccgcgcc catgcctgat caccctggag cctgccgcct gtgaaactcc
     1501 gcctcggcaa ctggatcgga ctcgccgccg tcgtggtcct gctggaccaa tggaccaagc
     1561 acctggtgcg cggcgccctg cgcaccggcg aggtgatccc cgtcaccgat ttcttcgacc
     1621 tggtcctggc tttcaatccg ggcgcagcct tcagctttct cgccgaccag gcgggctggc
     1681 agcgctggtt cttcgtggcc ctggcggcgg tcatctgcgg ctggctgctg cgtctggtgg
     1741 cgcggcacca gcatgaattc ctgcaaccgc tggccttctc gctgatcatc gggggcgcca
     1801 ttggcaatgt catcgaccgc ttcgtgttcg gcgcggtggt cgacttcctg tacttccacg
     1861 tcggccgcta cggctggccg gccttcaacg tggccgattc cgccatctgc gtcggcgtaa
     1921 cgctgatggt catcgcccaa ctgcgcgaac accgtcacgc gcccacccga gagacccttt
     1981 catgacccag accgtccagc ccgatagcct cgtcaccctc cactaccgca tcagcctgga
     2041 caacggccag cccctcatca gcaccttcga ggcccagccg gcgacgatgc atctgggcca
     2101 gggtgacatc gccccgacac tggaacgctg cctcgccggc ctcgccgtgg gcgaacgcca
     2161 cagcttcgaa ctggcacccg agaacgcctt cggcgcccac cgcgccgaac tggtcgagaa
     2221 ggtgcgcctg gaagacttcc ccgatggcgc cgaagtcgaa cccatggtca tcatggaatt
     2281 caccgcgccg gacggcagcc gctatcccgg cctgatccgc gaggtgctcg acacccacgc
     2341 ggtgatcgac ttcaaccacc cgcttgcggg caagcccatc cgcttcgaag tccagatcat
     2401 cggcatcaac tgaggttccc atggaagtcc tgctcgccac tccccgcggc ttctgcgccg
     2461 gcgtcgaacg ggccatcgag atcgtcgaac gggccctggc ccgcttcggc gcgcccatct
     2521 acgtgcgcca cgaggtcgtc cataaccgct tcgtggtgga aggcctgcgt gccaagggcg
     2581 ccatcttcat cgaggatctg gccgacgtgc cggccggcaa cacggtgatc ttcagcgccc
     2641 acggcgtgcc ccagtctgtc cgcagcgaag ccgaggcccg cggcctgcgc gtcttcgacg
     2701 ccacctgtcc gctggtcacc aaggtgcacc tggaggttgc ccgcatgcgc gaccagggcc
     2761 gcgagatcgt catgatcggt cacaagggcc acccggaagt ggaaggcacc atgggccagg
     2821 tcaaggacgg catccacctc gtggaatccg tcggcgacgt ggcgaccctg caggtgaaca
     2881 ccccggacgc ccttgcctac gtcacccaga ccaccctgtc ggtggatgac gcggccgcca
     2941 tcgtgggcgc cctgcgcgaa cgctacccgg ccatcgtcgg ccccaagaag gacgacatct
     3001 gctacgccac ccagaaccgc caggacgccg tcaaattcat ggcgccccag tccgatctgg
     3061 tgctggtcgt gggctcgccc aacagctcca attccaaccg cctgcgggag gtggccgagc
     3121 tgcttcaggt acctgcccac ctggtggaca acgccgacgc catcgacccg gcctggctca
     3181 cgggcaagca gcgcatcggc gtcaccgcgg gcgcctcggc gccggaggtg ctggtggagt
     3241 ccgtgatcgc ccgcctcaag gctttggggg cggcctccgt gcgacagctc gagggcgtgc
     3301 ccgagaaggt cacctttccg ctgcccaggg aactccagac gccggccgcc tgatctgccg
     3361 ccgtcaagct gctcgccccc tcgacggaag acgggcgcca cggccacaat acggcaccgc
     3421 cactccaccc accgtcccat gagcttcgtc cagcgcaacg ccgaaggctg catcgtcgcc
     3481 ctgtttgccg acgccgggcc ggggcactcc gaagagctgc cggccgatca cccggaggtg
     3541 gccgccttcc tcggggggcg tggcgcagcg gacggcgcgg tcaccttcac cgccctcgac
     3601 gccgacctga tccgcgtcat cgaggacgtg gtggacgtcc tcatcgaccg gggcctgctg
     3661 cgcctcaccg acctgccccc cgacgcccag cgcaagctcc tcgcccgcaa gggtgcgcgg
     3721 gaacgcctgc gcaacgggct caacctcctc ggcaagcacg acgtcatctg agcgcgacgc
     3781 agtggtcgcg cccaggggcc gccgcgtcgt tgacaccgag attttttgcc tgaaggctta
     3841 agttcacctt ctcgcttccg ttaggatgcg gagcggcgcg caggcgccct ccttcggcca
     3901 taccggtatc ccgcagcccg accatgacga atcccacgag ccgttccggc tggcagatcg
     3961 gcgagcatgc cacccacgac gaccccctgc tcgacgcgct ggtcctcctc acccgcatcc
     4021 acggcaaccc cttcaccccg gaagccctct cggccggcct gcccctcgtc gatcaccgcc
     4081 tgacgccctc cctgctgccc cgcgccgccg cccgcgccca gctcaccgcg cgcatcgtgc
     4141 gcaaaccgct ggcggagatc accccgaatt tcctgccggc gctgttgctc cttcacgacc
     4201 gccgcgcctg cctgctcctc gaacgcctgc cggacggcgc ctgccgggtg cgatttcccg
     4261 aagccggcga atcggtggac gtcctcagcg ccgacgagct cgccgcccgc tacacggggc
     4321 tggtgtgctt tgtccgtccg cgcttccgct tcgaggcccg tgcgccggag atccgcagtc
     4381 tgcgcggcca gcactggttc tggggcaccg tcctcgacaa ctggcggctg taccgggaca
     4441 ccctgctcgc cgccctcgtc atcaacctct tcgcgctggc catccccctg ttttccatga
     4501 cggtgtacga ccgggtcgtc cccaaccggg ccgtggaaac cctgtgggtg ctggcagtcg
     4561 gcacggcctt gctgctgtgc ttcgacttcg ccctgcgcac gctgcgcgcc tacatcgtcg
     4621 ataccgccgg aaagcgcatc gacgtgcagc tgtcggcccg catcatggag cgcgtgctgg
     4681 gtctgcgcat ggaggcgcgg ccggcctcgg tcggctcctt cgctgccaat ctgcgggcct
     4741 tcgagggcgt gcgggacttc atcgcctcgg ccaccgtcac caccctgatc gacctgccct
     4801 tcgtgctgct cttcctcctg gccatggcgt ggatctcccc gtggctgatg atcccgccgg
     4861 tggtcggcat cctcgtggtg ctgttcgtga ccctgatcgc ccaggacaaa atgcatgccc
     4921 ttaccgagac cacctaccgc gccggtgccc agcgcaacgc caccctggtg gaaagcctgg
     4981 tgggcctgga aaccctcaag accctcgccg ccgaaggcct catccagcgc cagtacgaac
     5041 gcgccaccct cttcatcgcc cagatcggcg gacgcctcaa gctgctctcc gccggtacgg
     5101 tgaatttcgc ccagttcatg cagcagttcg tctccgtcgc cgtcatcatc gtcggcgtgt
     5161 acgaactggc tgacgccaag atgagcctcg gcggcatcat cgcggcctcc atgctggccg
     5221 gccgcaccat ggcccccctc ggccaggtgg cggggctgct gatgcaatac cacaacgccc
     5281 gcacggcgct ggagaccgtc gaaggccaca tgcaactgcc ggtcgagcgc ccggacggcg
     5341 ccaacttcgt ccatcggcct gccttccgcg gcgagatcga gttcaaggat gtgagcttcg
     5401 cctatcccgg ccgcgatcag gcggccctgc acacgatctc cttccgcctg caggccggcg
     5461 agaaggtcgc catcatcggc cgcatcggct ccggcaagac caccatcgaa aagctcatcc
     5521 tcggcctcta tcagccgacc gcgggcgccg tcctcatcga cggcatcgac gcccgccagg
     5581 tggacccggc cgaactgcgc cgcgccatcg gcttcgtccc ccaggacgtg accctcttct
     5641 acggcagcct caaacagaac atcgccatgg gcgccccctt cgccgatgac gcggcagtgc
     5701 tcgccgcggc cgagctggcc ggcgtcaagg agttcgccga cgcccatccc cagggtttcg
     5761 acatgcccat cggcgaacgc ggcgaatccc tctccggcgg gcagcgccag gccgtggcca
     5821 ttgcccgtgc ggtgctcaac gatccgccca tcctgctgct ggatgagccc tccagcagca
     5881 tggaccacca gagcgaagag caactcaagc agcgcctgcg cgctttcgcg gccggcaaga
     5941 cgatcgtcct cgtcacccac cggacctcgc tgctggacct tgtggatcgg ctgatcgtcc
     6001 tcgaccaggg ccacatcgtg gccgacgggc ccaaggccca ggtggtcgaa gccctgcaac
     6061 agggccgcat cggacgggga ggctaatgcg atgaagtccc tgtttgcccg tctggccgcc
     6121 ttcattcagc ctcagtacgc gcgggtctat gatcggagct cccgcgcctt cgaccgtttg
     6181 ctggatggcg ccgaacgggg cgagatccac gacgacaccg actacctcgc cgacgccgaa
     6241 tgggcgatgg ccgagcagcg cctgcgcggc agccgcatcg ccgtgtggat caccctggtc
     6301 gccgtgctcg tgttgttgct gtgggccgcg tttgcccagc tcgacgaggt cacccgcggc
     6361 gaaggcaagg tcatcccgtc caagcaggtg cagatcctgc agagcctgga cggcggcatc
     6421 gtctccgaga tcctcgtcaa ggaaggccag caggtgaagg ccggcgagct gctcctcaag
     6481 gtggacccga cccgcttcgt ctcgtccctg cgggaaaacc gcgcccaata tctggcactg
     6541 ctggccaagg cagcccgtct caccgccatc tcccacggca agcccttcgt cgccccgccc
     6601 gaggtgctca aggacgcgcc ggatctggtg gagcaggagc gcagtgccta cgaatccaag
     6661 cgcgccgaga tggacgccgc tgccagcatc gcccgccagc aactgtccca gcgccgccag
     6721 gaactggctg aagtcagcgc ccgccgggat caatcgtccc agagctacga cctcaccgcc
     6781 cgcgagctcg aagtgacccg cccgctggcc aagaccggcg ccgtctccga ggtggagctc
     6841 ctgcgcctgg aacgggacgt ggcccgcttc aagggcgaac gcaacgccgc cgtcgcccag
     6901 atcccgcgca tccaggccgc catcgccgaa gcccagaaca aggtggagga agtggagctc
     6961 cagatccgca atcaggcgcg cagcgagctc tccgacacca acgccaagat cgccgccctt
     7021 tccgccggca gcgaaggcct cgccgaccgg gtcaagcagg ccgagatccg cgcgccgatg
     7081 aacggcaccg tcaagcagct cttcgccaac accgtgggcg gggtggtcca gccgggcaag
     7141 gagatcatcg agatcgtgcc cagcgacgac accctgctgc tggaagcccg cgttcagccc
     7201 aaggacatcg ccttcctgcg tcccggccag ccggcgctgg tgaagttcac cgcctatgac
     7261 ttcgccatct acggcggcct cgacgccaca ctggaacaga tcggcgccga caccatcacc
     7321 gacgaaaagg gcaatgccta ctacgtggtg cgcgtacgca ccgtccatag tgccctgggc
     7381 gatgccaggc tgcccatcat tccaggcatg gtggccgagg tggacattct caccggcaag
     7441 aagtcactgc tgagctacct cctcaagccc gtcctgcggg ccaaggccaa cgccctcacg
     7501 gaacgctgac gccgggtgcg ccttgcagag cgccagtcgg aaacgaagag gcccgcgcgc
     7561 gggcgggcct ctcatgtcat ctgccggcgt gtgcgccggt caggcgccgt ccacaccggc
     7621 acgtgaagcc agatacgccg cgaactccgc acccacttcc ggatgcttca ggcccagctc
     7681 gacggtggcc tgcagatatc cgagcttgga gccgcagtcg taacgcacac ccttgaactg
     7741 gtaggacagc accgcctctt cacgcaacag ggaggcgatg gcgtccgtga gctgcaactc
     7801 gccccccgcg cccggcgtga gcttgcgcag atgttcgaag atgcggccgg tgagcacgta
     7861 tcgccccacc accgcctggg tggacggcgc ctcttcgggc ttgggctttt cgacgatccc
     7921 cgtcacccgc tgcacatcgc cgtcctggcg ctcggtgctg acgatgccgt actggcgggt
     7981 gtgctcccgc ggcacgttca tgacgcccag caccgaacag cgatggtagt gatacacatc
     8041 cgccatctga cgcatcaccg atgaggcgcc ctgaccgtcc aggaggtcgt ccgccagcag
     8101 cacggcgaag ggctcatcgc ccaccaccgg cgccgcgcac agcaccgcgt gccccagacc
     8161 cagcgcttcc ggctggcgga tgtagatgca gttcacattc gccggcaccg tctcgcggac
     8221 gatcttgagc aattccgtct tgccccgcgc ctcgagttcg gtctccagct cgtaagcctt
     8281 gtcgaagtga tcttcgatgg cccgcttgga gcgcccggtg atgaacacca gatccgtcat
     8341 gccggccgcc accgcctctt ccaccgcgta ctggatcagc ggcttgtcca ccaccggcat
     8401 catctccttg ggcgacgcct tggtggccgg gaggaagcgg ctgcccatgc ccgccactgg
     8461 aaataccgct ttcgtaacct tcttcattcc tgatcctcaa ataagcttcc ctgaccgccc
     8521 ccgtcctgcg cgggcggcac cggctcaagc agggccatca gccccgcctc gtccagaatc
     8581 atgacaccca gttgttgcgc cttgtcgagc ttgctgcccg cctcggctcc ggcgacaagg
     8641 tagtcggtct tcttggaaac cgaaccgctg accttgccgc ccgccgcttc gatcaatgcc
     8701 gccgccgcgt cgcgcttgag tgtgggcagg gtgcccgtga gcacgaaggt cttgcccgcc
     8761 aggccgccgt cggccgagcg ggccgccgga gcgccctcgg tccagtgcaa ggcgtcggcc
     8821 agactgccga tgaccgcctg attacgggct tcgcccatga actcgacgat gcaccgcgcc
     8881 accaccggcc cgacatccgg cacctgcagc agctgtgcct catcggcagc catcagcgcc
     8941 ttcagtccac cgaaatgacg ggccagatcc ttcgcggtct tctcgcccac attgcggatc
     9001 cccagggcga agatgaagcg cgccagactc gtctcccggc tcgcctcgat ggccgccatc
     9061 aggttcgcgg cggacttttc gcccatgcgc tccagcccgg caagtacatt ggcattgatc
     9121 cggtcagggt tatacaggtc tgccggcgtg gtgaccgtgg aattcgtcac gagttggtgg
     9181 acgatctgct caccgagccc atcaatgtcg atggcacgcc gtccggcaaa gtgcagcagc
     9241 gcctgcttgc gctgggccgg gcagtagagg ccaccggtac accgcgccac cgcctcgtcc
     9301 tcgccccgca ccacgtggga gccacactcc gggcacaccg gataagtgcc aagcagatcg
     9361 aaacgcggac gggccacgtg ggccgcctcc tcctcctcgt tcccgatcac acggccgacg
     9421 acttccggaa tcacgtcgcc ggcgcggcgc accaccacgc ggtcaccgac gcgaagctcc
     9481 ttgcggtcga tctcgccctg gttgtgcagc gtggcattgg tgaccgtcac accaccgaca
     9541 aacaccggct ccaggcgggc aaccggggtc agcgcgccgg tgcggccgac ctgaacgtcg
     9601 atacccagca gatacgtcac ggcctcctgg gccggatatt tgtgcgccac cgcccaccgc
     9661 ggctcccgcg agacgaaacc cagcgcccgc tgcagggcca gactgtccac cttgtagacc
     9721 acgccgtcga tgtcgaaagg caggccgtca cgcaagacgc ccatcgcctc gtggaactgc
     9781 gcgagcgctg ccgccccttg ggcacgcgtc cggtgcacgc agaccgggaa gcccagggcg
     9841 tccagtgcat cgagcagctc ggcgtgggtc ggcggcagtt cccagcccac cacctccccc
     9901 agcccgtagg caaagaagga caggggccgc cgcgctgtaa tggccgggtc gagctggcgc
     9961 aggctaccgg cagcggcatt gcggggattg acgaaggtct tctggccggc ggctgcctgg
    10021 tccgcgttga gacggtcaaa cgcgtcgcgg cgcatgtaga tctcgccgcg cacttccagc
    10081 accggtggca gcgcgccctt cagccgcagg ggaatctggc ggatggtgcg cacattgcgc
    10141 gtcacatcct cgcccgtcgt gccgtcgccc cgggtggccg ccagcaccag ggtgccacct
    10201 tcgtagcgca gattgatggc gagcccgtcg aacttcagct ctgccgcgta ggtcaccggt
    10261 ggcgcgtcgt ccgtcagccc gagggcccgc cgcacgcgtg tgtcgaagac aacggcgcct
    10321 tcggcggtgg tatcggtctc ggtgcggatc gacagcatcg gcaccgcgtg actcaccggc
    10381 gccagctccg gcgccggctg gccgccgaca cgctgggtcg gcgaatcggg tgtcaggagt
    10441 tccggatacg tcgcctcaag cgcctgcagt tcacggaagc gggcatcgaa ttccgcatcc
    10501 gggacaaggg gctcatcgag gacatagtag gcgtggttgt agcgttcgag cgcctcgcgc
    10561 agggccgccg cccgcgcgat ctgttcggcc gggacggaca cggcgcgcgg tcaggcgaac
    10621 aggcgccgcg ccagtgtcga acccggcgcg atgccctgct cgcccatctg cacctgaaac
    10681 tgctcgatct gggcgcggat caggcccgca gccttgtcac cgaaaggcgt gcggttgtcg
    10741 tccaccacgg cgccgttgag ggcttccgcc agttgctggg ccaccgtcat catgcggtcg
    10801 aagaccttca cgccatccgc cacccgcggc acgtcgagcg tcagggtgac gccctgggta
    10861 ctcaggccac gcatctcctc ggccacgaat cggaccggct ccaggttgct cagcacgaac
    10921 tggctcaggc ccagatcgtc acgggcgtga aagcagccgt ccgtgcccag ttccagcccg
    10981 gccgcctcag ccaggccgcg cagcttggtg ccgggaatcg gcagttcgcg ggccaccaca
    11041 ttgacgccga tctggatgtc cacgccggca cacaggcgat ccagctctgt ggcccggtcc
    11101 agggcttcgg ccttgaccgg cagggacgga atgcccatga agatgtcaca catgcgctgc
    11161 agtccgccgc agaaggccga cagatcccct tcgctcaccg gcccgcggcg atccaccagc
    11221 tgcagcgccg cacagatcca gtgatagccg tggccgggcg caccatccag cgtttcccag
    11281 cgattgtcgt gatccgaaaa gccgaaccag cgcaccggct tggcgatgcc ctgcagatgc
    11341 tgcgctgcgg cctggagcag gcggctgccc tgcacgggct cgatcgattc cactcgcacc
    11401 acgcagtcgg cccgctcgtc cacctcggcc ggcaagggcg gcggcacgcg acgcggtccg
    11461 gcggtgggcg ccggacgggc ggccacgggc cggggcgtcg ccgcaccgag caccggctcg
    11521 atacgcgcct cgccaccgcg ggccgccgtc ccggaagcct ggggctccgt cggcacaggc
    11581 ggctcggcga gtttgcgctg ccggcgctcc tgccacttgt tgaacgcgaa gatgcccagc
    11641 acgaccgcac cgccggcccc gatcaggccc atctgcaatt cactcattcc catctgcttc
    11701 cctttgtttc acactcacgc cgccgtttcg gccaggcgca tggcttcttc gatatccact
    11761 tccaccaccc gcgacacgcc ctgctcctgc atcgtcacgc ccaccaactg ctgggcgatc
    11821 tccatcgtga tcttgctgtg gctgatgaag atgaactggg tctgggacga catccgcttc
    11881 accatcccgc aatagcgccc ggtattggca tcgtcgaggg gggcatccac ttcgtccagc
    11941 atgcagaacg gcgccggatt gagctggaac atggcaaaca ccagcgcaat cgccgtcagc
    12001 gccttctcgc caccggacag cagctggatg gaactgttct tcttgcccgg cggctgggcg
    12061 acgatctgga tgcccgcgtc gagaatctcg tcgccagtca gcaccagccg cgcctcgccg
    12121 cccccgaaaa gctgggggaa caggctgccg aaatggcggt tgaccgtatt gtacgtctcc
    12181 tgaagctgct cccgcgtttc ccggtcgatg cggcggatcg cgtcttccag ggtctcgatg
    12241 gcccccagga gatcctcgct ctgcacatcc aggtaggtct tgcgctcgga cgccgccctg
    12301 agctcgtcca gcgcggccag gttcacggcc cccagttccg cgatctcgcg gccaaggcgg
    12361 gccacttcgc gctgcaaggc gccttccttc aggtccgccg tgagcagcgg cgcgagggcg
    12421 gcttcgtcgg cctgcgcttc ctccagacgg gtggcgaact gggcctcggc cagctcggcg
    12481 gcctgaacgc gcagacgcag ctccgcaacc cgctcgcgaa cgggggccgc ggtctgttcg
    12541 gtgtggtgac gggcttcctc caggccgcgc acctgggcgt ggatcgcctc cagcccgttg
    12601 cgcacctcga ccagcgccat ctcccgtgtg ccgcgcagga ccaccgccgc ctggagcgcc
    12661 gcggccaccg agggctcgtc gatctccagc aggctttcgc ggcgcgcttc cagctcttcg
    12721 gcggtgcgcg ccagctgctc ttccgccagg ctacggttgc gggcgatgtc gtccagcttg
    12781 ctggtgcatt cccgttctgc gtggcgggcg tcctgcagct cgcgggccag ggcctgctcc
    12841 agcgcgcggg tctcgcgcag ggctgcctcc cgctcgcggc gcaccgcttc ggcggcgtcg
    12901 aggcgtccgc gttgcagctc ggccatctcg cggctgcgtt ccagctcggt ctccgagcgc
    12961 atcaggcggt cgcgctcggt ctgctccagc aaggcgatct cgcccatgtc ccgttcgatc
    13021 tgggcccagc gttcctcgta acggacccgc gcctggttga gtttgagcac ctccacctgt
    13081 tcgccatgga cctgggcctg cagggcctgc acgtcgcgac gcagggtggt gaggccgtcc
    13141 tgcagccgcg tcgcggtggc gtcagcctcc gccacggcct ccttggcctg cgccgcagcc
    13201 tcctgggccc ggtcgagctc gacggcgagc tggtcgatct cccgctggcg ttcgatcacc
    13261 ccgtgggtac gggcgtccgg cgcatagtgg atcagggcgt ggcggtccag cacgcgaccg
    13321 tccttggcca ccagccggac ggaaggcgac agttcggcgc gccgttccag ccagggcgcc
    13381 agatcgtcca cggccagcac cgtgcccagc caggctgcca gcaccggcgt cagcgtcaca
    13441 tcggcacaga catgatccag cagaggccgc gtgccttcca gcgccatggg cggcgcccca
    13501 tcagccggcg catcggccag caggaaggcg aacgcgccgg gcaccttgtc ggccaacgca
    13561 atcagcgccg cgcgcccgtc ctcggccggc agggccgcga ggcgttcccg caggatggcc
    13621 tccaccgccg attcccagcc ggccgccacc tgcagccgct gccacagggg cgtgaggccg
    13681 tcgaggcgat ggcggcgcag ccagtcgccc aactggccct gggcctggac cttgtgctgg
    13741 agctgcacca gggcctcgcg gcgggcacgc agttcggtgg cctgccgctg agtctggcgc
    13801 tcggcttcca tggcctcccg cagggcctgc tgggcgccgg gcagcccggc ctgggcgtcg
    13861 gccacggcac tctggcgggt ggccagggta tcttcgagct ccgacagcag ggcctcccgt
    13921 tcggcgattc ggcgcagatc cggcgcatcc accgcggact gctcctgttg caggcgaccg
    13981 cgtcgctggc cgagggtctc gagggtccgc tcgctgctga cccggtgggc ctcctcgaca
    14041 cggatctgct gttccgcctg gttcagctca cggcgcaccg ccgccgcggt ggcatcggcg
    14101 gtgcgcaggg cgtcctcggc ttcgggcaga cgctcggcga gttcggcatg acgggcctcg
    14161 gcctgctcgg cacgcagggc cgcgtgttcc tgcaggctgt gccagcgttc ggcgtcactg
    14221 ctcagggtgt cgagctggct gcgccactgg gcgtcatccc gctccaactg caccagacgg
    14281 ctctccagca cgcgccggga atcccgcagg cgggccagtt cgccctccag gcgggtcagt
    14341 tcggtactga ccccatgcag ggcatcctgg gcgtgctgga cgtgaccgga ggcagcaaac
    14401 tgggcctcgc gggcgctttc cagctggctt tccagcgtct gcaggcgggc cgagtcggcc
    14461 tcgatccggc gcgtggcctc accgagttcc tccgtcaggc gggtccgctc gctgcgggcc
    14521 tcgttgcgct tgatcagcca caggagattc tggcgctccg acaggcttgc gttgagccgg
    14581 tggtagcgct cggccaccgc ggcctgggcc tctagcctgc cgatctgctg accgagctcg
    14641 tggcggatgt cctccaggcg cgccagattg tcgcgggcgt cggtgagacg gccttccgtc
    14701 tccttgcggc gttcccggta cttggtgacg ccggcggcct cttccaggaa gccgcgcacc
    14761 tcctccggcc gcgcctcgat gatccgcgag atcatgccct gctcgatgat ggcgtaggcg
    14821 cgtggcccca ggccggtgcc gaggaacagg tcgatcacgt ccttgcggcg cacctgcagg
    14881 ccgttgatga aataggtgga ttcgccgctg cggtcgagca cgcgcttcac cgccacctcg
    14941 gcgtagggct tccactgccc tgccgcgcgc ccctcggcgt tgtcgaagac cagttccacg
    15001 ctggcccgcg acaccggctt gcgggtggtg gaaccgttga agatcacgtc cagcattgat
    15061 ccgccgcgca gcgccgacgc ccgcgattcg cccagcaccc agcgcacggc atcgatgatg
    15121 ttcgatttgc cgcagccatt gggaccgacc acgccgacca gattgcccgg cgtgagcacg
    15181 gtggtcgggt ctacgaaggt cttgaaaccg gcgagcttga gtttggcgag acgcacgtgg
    15241 tcagggtgtc aactgggggc cgccagggcg tgccgcggag aatttctcga gggcgcgggc
    15301 agcgcattgc tgcactgccg catgtcaggt ggccgctata ataccattgg cccttgacga
    15361 ggccagccgg cgcgttttcc gcgccgttca ccgcctttcc cgcccggatc cgggccgact
    15421 tcaggatctc cagccgaccg tgaacccgca cctccaggat ctgcacccct accccttcga
    15481 aaagctccgc gccctcttcg acggcatcgt cccgcccgcc gaatactcac cgatccgcct
    15541 gtccatcggt gaaccccagc acgccacgcc ggccttcatc cgcgacgccc tggtggccca
    15601 tctgggcggt ctcgccaact atccgacgac gcagggctcc gacgccctgc gcaagtccat
    15661 tgccgcctgg ctggaatccc gctacggtct gcccgccgtg gatcccgcca cccaggtgct
    15721 gccggtgaat ggctcccgcg aggcactgtt tgccttcgcc cagtgcgtca tcgacggcac
    15781 ccggcccgac gccctggtgc tgtgtcccaa cccgttctat cagatctacg aaggggccgc
    15841 ctatctggcc ggcgcccagc cgtatttcat caacaacctg ccggacgaca atttcggatc
    15901 cgacttcgcc cgcgtgcccg acgaggcatg ggcccgcgtg caactggtgt atgtgtgttc
    15961 gccgggcaac cccacgggcc gcgtcctcac cctcgacgaa tggcagcacc tgttcgagct
    16021 gtcggaccgc catggcttcg tcatcgccgc cgacgagtgc tactccgaga tctacttcaa
    16081 cgacgacgcc ctgcccatcg gcggcctgga agccgcccag cgcctgggcc gcagcgactt
    16141 ccgcaacgtg gtgatgttct ccagcctgtc caagcgctcc aatgtgccgg gcatgcgctc
    16201 gggcttcgtg gccggcgacg cgaacatcct caagcgcttc ctgctctacc gcacctacca
    16261 cggctgcgcc atgagcccgg tggtacaggc cgcctccgcc gcggcctgga gcgacgaagc
    16321 ccacgtggcc gaaaaccggc gtctgtaccg ggaaaagttc gaccgcgtca ccccgctcat
    16381 cgccgaacac ctggaggtga gcctgcccga cgcgggcttc tacctgtggg cgcgggtgga
    16441 caagctgggc ctctcggaca ccgaattcgc ccgccgcctg caggccgaat ataatgtgac
    16501 ggtcctgccc ggcagtttcc tggcccgcga ggcccacggg accaaccccg gtgcaggctt
    16561 tgtccgtatc gccctggtgg ccgaactggc cgagtgcctc gaagccgccc ggcggattgc
    16621 cgcctttgcg caacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggca
    16741 gcttcatcgg caagggcgtg gtgctgatgc cttcctacgt gaacatcggc gcgttcgtgg
    16801 atgaaggcac catggtggac acctgggcca ccgtcggttc ctgcgcccag atcggcaaga
    16861 acgttcacct ctccggcggc gtcggcatcg gcggcgtgct ggaacccctc caggccaatc
    16921 ccaccatcat cggcgacaac tgcttcatcg gcgcccgctc cgaagtggtg gaaggcgtca
    16981 tcgtggagga caactgcgtc atctccatgg gcgtgtacat cggccagagc accaagattt
    17041 acgaccgcac cactggcgaa gtgctctacg gccgcgtgcc cgagggctcc gtcgtggtga
    17101 gcggcaacct gccctctggc gacggcaagt acagcctgta ctgcgccgtg atcgtcaaga
    17161 aggtggacgc ccagacccgc tccaagacca gcatcaacga actgctgcgc gactgacctt
    17221 cccccgggcc gcggtcacgc ggcccgtttc attcgagccg ggcccaccga tgatcatcga
    17281 caagctgttc accctgatgg ccgagaagaa ggcctcggac atctacgtca cggtgggtgc
    17341 gcccatccat atcaagatcg acgggaacac cctgccgatc aatcagcagg ccatggacgc
    17401 gggcatgatc caccgcatgg cctacgagat gatgacgccg gagcagatcg cccgcttcga
    17461 gaacgaacgg gagatgaacc tctccttcag ccggcgcaac atgggcaact tccgcgtcaa
    17521 catcttctgg cagcggaaca gcattgccat cgtcgtccgt tacatcctcg accagattcc
    17581 ccgcctcgac gccctcggcc tgccacccgt gctgtccgac gtgatcatgg aaaaacgcgg
    17641 cctcgtgctc gtcgtgggcg ccaccggctc gggcaagtcc accaccatcg cgtcgatgct
    17701 ggaccaccgc aacgagaact tctccgggca catcctcacc atcgaggatc ccatcgaata
    17761 cctcttccgg cacaaggcct ccatcgtcaa tcagcgcgag atcggcaccg acaccctgga
    17821 ctggaaccac gccctgtcca acgccatgcg ccaggcgccg gactgcatcc tgatcggcga
    17881 gatccgcgac cgggacacca tgcaggccgc catcgcctac gcccagaccg gtcacctgtg
    17941 cctggccacg ctgcacgcca acaacgctta ccacgcactc aaccggatct tcaacttctt
    18001 tccgatggaa aaccgcccgc tcctctacat ggacctggcg gcgaccctca agtgcatcat
    18061 ctcccagcgc ctggtcaagc gcccggacgg taaacgcatc cccgcggtgg aaatcctcct
    18121 caactcccgc caggtggccg aactgatcga gcgtggcgag atctccggca tccgcgaggc
    18181 catggagcag agcctcgcct ccggctcgcg caccttcgaa caggatctct tccgtctgta
    18241 ccgggacaag atcatcaccc tcgaagaagc cctcgccaac gccgactcgc ccaccaacct
    18301 gtcctggctc atcaacaacg cccagttcag cgacaagaac gccgacaaga ctcccttgcc
    18361 gtccacgtcg atcgacagcg aaccgcatca ggaagaaggc ggcgcgtcgt tcaaggagtt
    18421 cacgctcgaa ctcgacgaag actgacccag ccccgacccc acacccatga cccgaccgac
    18481 agccccggcg agcgccacgc tcgccctgac ccgtgaactc atcgcccgcc gctccgtcac
    18541 ccccgaggac gccggctgcc tcgacctgat cgcctcacgg ctcacgcccc taggctttgt
    18601 ctgcgaacgc ctcgatgcgg gcggtacctg caacctgtgg gcgcgccgcg gcacggcccg
    18661 cccgctggtc gtcttcgccg gccacaccga cgtggtgccc accggccccc tcgcccagtg
    18721 gcagtccgat cccttcgaac ccaccgaacg ggacggtttc ctctacggcc gcggtgccgc
    18781 cgacatgaag acctccctcg ccgccttcgt cacggccatc gaagacttcg tcgccagcca
    18841 cccccatcat cccggctcca tcgccctcct cctcacctcc gacgaggaag gcgacgccac
    18901 ttacggcacc gtgcacgtgg tgaacaccct caaggcacgc ggtgagaccc tggacttctg
    18961 catcgtcggc gaacccacgt ccgtcgacgt gctcggcgac atgatcaaga acggccgtcg
    19021 cggcagcctc tccggcaagc tcacggtcaa gggcgtccaa ggccatatcg cctaccccca
    19081 cctcgccagg aatcccattc acctggccgc accggccctg accgaactcg ccgccacccg
    19141 ctgggacgaa ggcaacgaat actttccgcc cacctcctgg cagatgtcca acatccacgc
    19201 aggcaccggt acgaccaatg tcattcctgg cacgctggag gtcctcttca acttccgctt
    19261 cgccacggtg cacaccgcag acgccctgaa gcagcgcgtc cacgccatcc tcgaccgcca
    19321 cgggctcgac tacgacctcg cctggaccct cggtggcaag cccttcctga cgccccgcgg
    19381 caccctggta ggcgccctcg aagccgccat ccgcgatgcc ctcggccgca cgcccgaact
    19441 ctccaccacg ggtgggacct ccgatggccg cttcattgcc gacatctgtc ctcaggtggt
    19501 cgagttcggc cctgtcaacg ccaccatcca caagatcgac gagtgcatcg cactggatgc
    19561 cgtcgacccg ctgtcggaca tctaccgccg caccctcgaa cacctcctcg cctgaccgga
    19621 gcacccatgg cccacgaaga cacccttccc gacgacgccg ggcacgacga gcacgaacac
    19681 gaacacgagc atggccccct cgccgagctc gtcaccgtcc gtgactggct gcgctatgcg
    19741 gtcacccgtt tcaaccgcgc cggctgcttc ttcggtcacg gcttcgtaga cgcctgggac
    19801 gaggccgtct atctcgtcct ccatacgctg gccctgcccc tggaccgcct cgaccccttc
    19861 ctcgacgcct gcatccccgg cgatgagcgg gaagcaatct tcgatgtcat cgaaaagcgc
    19921 gccgtcgacc gcctgccggc cgcctatatc accggagaag cctggctcgg cgagttccgc
    19981 ttcgagatcg acccacgggt catcattccc cgctcctatt tcgccgagct gctccacgaa
    20041 ggcttcagcc cgtggatcca ggatcctgcg agtgtcacgc gtgccatgga catgtgcacc
    20101 ggctccggct gcctcgccat cctgatggca cacgcctttc cccatgcaga gatcgtcgcc
    20161 gtcgacattt caccggacgc gctggaaatt gccgcccgca acgtggccgc ctatggcctg
    20221 gaggaccgca tcaccctggt ccagtccgat gccttcgacc tggtgccgga acagcccttc
    20281 gacttcatcc tcagcaaccc gccctatgtc acccaggacg cgatggacga tctgcctgcc
    20341 gaataccggc acgaaccggc actcgccctg ggcgccggcg aggacggcct cgacctggtc
    20401 cgtcgcctgc tcgccgatgc gccccgtttc ctcgcgccgg acggtcagct cgccgtggaa
    20461 gtcggacaca atcgggatct cttcgaagcg gccttcccga ccctcgctcc gacctggctt
    20521 tcggccccca gtggcgacga aaagatattt ttgataaatc ggcgtcaatt gttgtaaaag
    20581 cacccgataa acgccaactc cgtccttact gaaactacgg acagctcctt atgatcgtac
    20641 ttacccggaa aggggaccgc cagcggttaa agaacgacat aaaaagaccg aaaagcaagg
    20701 tcaaggagct caaaagatgg cgaccgatcg ccccatactg ctagtcgaag acaaccccga
    20761 cgacgaagca ctgaccctca gggccttcag caagaacaag attgccaatc aggtcgtggt
    20821 cgctcgcgat ggcgtcgaag cgctcgacta cctcttctgt acgggtcagt attcaacgcg
    20881 cgacagccac cagatgccgg ccttcgtact gctcgacctc aagctgccgc gcatcgacgg
    20941 ccttgaggtg ctgcgtcgca tccgcgctga cgagcgcact gccctgttgc cggtggtcgt
    21001 cctgaccacc tccaaggaac accaggacat cttcgaggcc taccgcctcg gcgccaacag
    21061 ctacatccgc aaaccggtgg atttcgagcg tttcctgcag gctgtgggcc agctgggcct
    21121 gtactggctg tccctcaacg aaccgtccga tccgacggca cgctgatccc ttacagcagg
    21181 gcttcaatat cgccagccag ttcggctggc ggcgtggtcg gcgcatagcg ccggaccgga
    21241 tttccgacgc gatccaccag gaacttggtg aagttccact tgatggcgcg ggatcccagc
    21301 acccccggca gggccgccgt gaggtaatga aacaggggat gcgcgtcggg accattgacc
    21361 cgcagcttgt ctgccatggg aaaacggaca tcgaaacgcc cggtgcagaa ggccccgatc
    21421 tcctccgcac tgcccagttc ctgccctcca aactggtcgc atggacacgc aatcaccacc
    21481 agaccctgcg caccataggt ttcgtacaag gcctgaagcc cggcgtactg gggcgtgaaa
    21541 ccgcaacggc tggctgtgtt cacaatcaac agcaccttgc ccgcgtagtc ggccagacgc
    21601 agcacgccgc cctgcagggc cggcagttca aaggcgtgaa gctgtgcgcc gccgctcatg
    21661 ccggtctcgc tggccgcatg ctcaggcgac ttcgtcgatc cacgcctgct ggatcgcttc
    21721 gaggatgcgt tcgccacaac gcttcgggtc gtcgtcgaat tccggtaagg ccatgaccca
    21781 gctcatcagg tcaacaaagt tcacccgtgc cggatccgcg tccgggcggg cttcagacaa
    21841 ctggatggca atttcctgca cgtcggtcca tttcatcccc gcttgccctc cttgaccatg
    21901 ttgatggaat agcgcgggat ctccaccgtc agcggtgtac cgcccaccag cgcctgacag
    21961 gacaggcgcg actggggctc cagtccccag gccttgtcga ggagatcctc ctcttcgtct
    22021 tccgccggtt ccagggtgtt aaatccgtcc cgtacgatga cgtggcaggt cgtacaggcg
    22081 caggactgtt cgcaggcgtg ctcgatggcg atgccgttct ccagcaggct ctggcagatg
    22141 gactcgccgg gacgggcgtc gatgaccgcc ccttccgggc acagctccac gtggggcaga
    22201 acgatcagtg tggtcatgat gaatgcagtc gttgaacgga tcagatccgg atgtcgtcga
    22261 tcttgtggcc ggacaaggcg gcgcggatac tccggtccat gcgccgggat gcgaactcgt
    22321 tggaggcccg gttgaaggac tcaagggcca gtttgatggc ccgcgcgtcg aggccggccg
    22381 cggcactgcg cagtgcctcc accgaggcgt ccagggcggt ccgctcgtcc gccgtcaaca
    22441 ggtcgccatc cgacatcagc gccttttcgg tcgcctcgat gacccgtgcc gcttcgacct
    22501 gctgctcctt aaggcctcgc gcctccatgt cggcccgggc gtactcgaag ccggactgga
    22561 gcatgcgcgt gatctcatcg tccgacagtc cgtaggaggg cttcacctcc acgcgggcct
    22621 cgaccccgct gagggtctcc cgcgccgaca cggacagcag gccatccgca tccacctgga
    22681 aggtcacgcg gatgcgcgcc gcccccgcca ccatgggcgg gatgccgcgc agttcgaagc
    22741 gcgccagcga gcggcattcg ctcacgagct cccgctcgcc ctgcaccaca tgaagggcca
    22801 tggccgtctg accgtccttg aaggtggtga agtcctgggc ccgcgccgtc ggaatggtgg
    22861 cattgcgggg aatgatcttc tccaccagtc cgcccatggt ctcgatgccc agagacaggg
    22921 gaatcacatc cagcagcagc cagtcgtcct cggccgcccg gttgcccgcc agcacgttgg
    22981 cctggatcgc tgcaccgatg gcgacgaccg tgtccggatc cagattggtg aggggcgtct
    23041 ggccgaagta ctcgcccacc gccttctgca catggggcat gcgtgtcgca ccgcccacca
    23101 tcaccacacc cttcacgtcc tcgggcgcga gccctgcgtc gcgcaaggcc ttgcggaccg
    23161 gcgcgagggt cttgctcacc agatgacggg tcatctcgac aaactgctct cgggtgaggg
    23221 tcaggtccac ctcatcgcca cgggtgaggg tcagacggat gtctaccgat tcgtcggtgg
    23281 tcaggcgttc cttcacttcc cgcgccttca tctgcaggcg gcgcgcgtcc tggtcggacg
    23341 gcagcgagat gccggcgttg tcgaggatcc agcagaacag acgatggtcg aaatcgtccc
    23401 ccccgagaac cgcatcgcca ttggtggcga ccacctcgaa cacgccgcgg gacaggcgca
    23461 ggatggagat gtcgaaagtg ccgccgccca gatcgtatac ggcatacacc ccttcggacg
    23521 cgttctccag gccgtaggcg atggccgccg cggtgggctc gttgagcagg cgcagcacgt
    23581 tgagcccggc gaggcgcgcg gcatccttgg tggcctggcg ctgggcgtcg tcgaaatagg
    23641 ccggcaccgt gatgaccgcg ccggtgagtt cgccgcccag gctgcgttcg gcgcgcagac
    23701 gcaggctgct gaggatctca gccgacactt ccaccgggct cttcaccccc tgaaccgtgc
    23761 gcaggcgcac catgccctgc tggtccacga agtcgtaagg catggtctcc acgtgggcca
    23821 cgtccttgag accacggccc atgaagcgct tgaccgagac gatggtgttg cggggatcaa
    23881 cggcctgggc cttttgggcc ccacggccca cggcaacccg tccgtcgggc aaatactgga
    23941 ccaccgaggg cagcagcacg cgcccgtcgt catcgtgcag acattgcggc acgccattgc
    24001 gcaccgttgc cacgagggaa ttggtcgtcc ccaggtcgat gcccaccgcc aggcgatgct
    24061 ggtgcggcag cgtggacaga ccgggctcgg aaatctgtaa cagggccatc acgaatccag
    24121 tgacgccagg gcgtcgttga tttcatcttg caggcgttcg aggaacttca gccgccgcac
    24181 cgtatccgcc gctgccggga gatcgccaag cgtgtcgatc tcgtcaccga tctggacgac
    24241 cagcgcgcgg gaacgctcac ggatgcgccg cgccatgtcg tcaagggcgg acgcgtcggc
    24301 cgcttcacgg gcctcgccaa gggcttcgcg ccactccatc tgttccatca ggaactccgg
    24361 cgccatggcc gtgttgttct cgaaggccgg atccacgccg cgcagctcca gcaggtactg
    24421 ggcgcgggtg agcggctggc gcagggtacg gaaggccccg ttggcgtggg tggcccactg
    24481 catggactga cgcttctcct gatccgacag atgggcgaag cggtccgggt gaaccttgcc
    24541 ctgaagggtc aggtaggcct gctcgagctg atcgagatcg atgtggaaag ccggcgcaag
    24601 gccgaacagg gcgaagtggt cctggcgcag gtcgatcatg tcagaccgtg aagctttcgc
    24661 cgcagccgca ctggtccttc acgttcggat tgttgaactt gaagccctcg ttgaggcctt
    24721 cacgcacaaa gtccagctcg gtgccgtcca tgtagggcag gctcttcgga tccaccagca
    24781 ccttcaggcc gcggctctcg aagaccacgt cgtcggactg cacctcatcc gcaaattcaa
    24841 gcttgtaggc cattccggaa cagccggagg tgcgcacccc cagccggatg ccgatgccct
    24901 tgccccgctt cgcgatgaag ttgtcgatgt gctgggctgc cttttcggac agggtgactg
    24961 ccatgattgc cttcctttca accgttgtgc ttcttcttgt aatcctcgac cgccgccttg
    25021 atagcgtcct cagccaggat ggaacagtgg atcttcacgg gcggcagggc cagttcctcg
    25081 gcgatggcgg tgttcttgat ctccagcgcc tggtcgaggg tcttgccctt gacccattcg
    25141 gtcacaaggg aactcgacgc gatggccgag ccgcacccat aggtcttgaa cttggcatcc
    25201 tcgatgacgc catccttgcc caccttgatc tgcagcttca tcacgtcgcc gcaggccggt
    25261 gcgccgacca tgccggtccc cacaccgtcg tcttccttct ggaacgaacc gacgttgcgc
    25321 gggttttcgt agtggtccag aaccttttcg ctgtaagaca tcttcgttct cctgtcagtg
    25381 ggcagcccat tgcacggtgt tcagatcgat gccctcctgg accatctccc acaggggcga
    25441 cagttcgcgg agcttaccga tcttgcgctg caacaaatca attgtatagt cgatttcctc
    25501 gaccgtcgta aagcggccga tggtgaagcg gatggaactg tgggccagct cgtcgttgcg
    25561 gccgagggca cgcagcacat aggacggctc caggctcgcc gacgtgcacg ccgacccgct
    25621 cgacaccgcc acgtccttga tcgccatgat cagggactcg ccttccacgt aggcaaagct
    25681 catgttgagg ttgtggggca cacggtgcac cagatcgccg ttcacgaagg tttccggaat
    25741 ggcggagagc ccttcgaaca agcggttgcg cagggccagg atgcgcgcgt tttcctgggc
    25801 catttcctca cgggcgatgc ggaaggcctc acccatgccg acgatctggt gagtcgccag
    25861 agtgccggag cgcaggccgc gttcgtggcc accaccgtgc atctgcgctt ccaggcgcac
    25921 acggggcttg cgccgcacgt agagggcgcc gatgcccttg ggaccatagg tcttgtgggc
    25981 gctgaaggac atcagatcca ccttcagcct ggccaggtcg atgtccacct tgccggtggc
    26041 ctgggccgcg tccacgtgga agacgatgcc cttctcgcga cacagctcgc cgatctcggc
    26101 gatgggctga acgacgccga cctcgttgtt cacgaacatc accgacacca gcaccgtatc
    26161 cgggcggatc gctgccttca gcacctccag gtcgatcagg ccgttgtcct gcacatccag
    26221 gtaggtggcc tcaaagccct ggcgctccag ctcgcgcacc gtgtcgagga cggccttgtg
    26281 ctcggtcttc acggtgatca ggtgcttgcc cttgcctgaa taaaagtgcg ccgcgccctt
    26341 gatggccagg ttgttggact cggtggcgcc ggaggtccag atgatctcct tcggatcggc
    26401 gtttaccagc gcggccacct gttcgcgggc ctcctcgacg gccttttcag cggtccagcc
    26461 gaaggcgtgg ctgcgggacg ccgggttgcc gaacaactcg gtgaggtaag gaatcatctt
    26521 ctccgcgacc ctcggatcca cgggggttgt cgccgaataa tcgaggtaga tggggaactt
    26581 gagcatattc atcgtctccg cactgggtaa tcaagccgcg tctgcgactc cgaccgccat
    26641 cgcggtcagg tttctgaggt ggtcgacgat ctgcgcgatc gcctccagga atccatctac
    26701 atcctgcccg gtattgcccg agcccaggct cacgcgcacc gccgcacggg cacactccgg
    26761 cgtcacgccc atcgccagca gtgtgggcga cggctccggg tgggcgctgg agcaggccga
    26821 accgctcgcc accgcaaaac cggcccggtc gagcttgccc accagggtct cgccatcgat
    26881 gcccggcagg gcaaagaaag tggtgttggg caggcgctcg gcgccggccg aaaagatcgt
    26941 cgcccccagc ccggtcaatc cggcatccag ccgcgcctgc aggccccgca gtaccgccgc
    27001 ccggtctgcc tggcgtgccg ccgccagctg gcaggccttg ccgaaaccga caatggccag
    27061 cacgttctcg gtgccggagc gcagatcgcg ctcctgaccg ccccccgcca ccagcggcgc
    27121 gagctcgacc cgcttgtcca caatcaacgc gccggcgccg acgggaccat tcaccttgtg
    27181 tgcagacagg gacataccgt gcacgcccag cgcccggaaa tccacgggga gcttgccgaa
    27241 ggcctgaacc gcatcggtat gcatccatcc acccgcggca cggacctgcg ccgccagccg
    27301 ggccacgtcc tgaacgacac cggtctcgtt gttggccagc atcaccgaca ccagccgggg
    27361 ccgctcagcc agcaccgctg taaaggccgc actgtcgatc cggccggccg cgtccaccgc
    27421 gagctcgcgc accgtccagc ccgactggcg caactgccgg gccggctgcc gcacgcaggg
    27481 gtgctcgatg gcaccaaggg ccagcgcacc cgccttgagc cgggccgccg cccccttgag
    27541 gaacaggtta ttggcctccg acccgccgct ggtgaagatc acctcggtgg gatgggcgtt
    27601 caccgcttct gccacccggg cacgcgcctc gtcgatggcc atgcgtgcgg cgcggccgta
    27661 ctcatgacgg ctcgacgcgt tgccgaagcg cccgcccaac cagggcaaca tcgcctcacg
    27721 cacctcggga tcgaggggcg tggtggcgtt gtggtccagg tagatcgggg caaacatggc
    27781 ggacctgcgc ggttcaggct gtcagcgact ggccggcctc gtggcgggcg attgcggcac
    27841 gttcgtcatg cagcacggac accgccaggg ccttctgccg ctgggcgtca acaagactcg
    27901 ccagggtcac cgactgcaga tattcctgca tgcgccggtt gaggttggtc cacagatcgt
    27961 gggtcatgca gcgatgctcg tcgagacagt tttccttgcc gccgcaggac gtggcatcca
    28021 gaggctcgtc caccgcggcg atgatgtccg cgaccgtcac cagttccatc ggctgggcca
    28081 gggtgtagcc gccaccgggt ccgcgcacgc tggacacgag ttcgaagcgg cgcagcttgc
    28141 caaagagttg ctcgaggtag gacagggaaa tccgctgccg ctcggcgatg ccggcgagcg
    28201 tcacgggtcc gtcggcacac cgcagggcca gatcgatcat ggctgtcact gcaaaacgac
    28261 ctttggtggt cagtctcatc gtcactcccg ggggctggta ggggaatagt tgaatatttc
    28321 agtcaagaat agcaaacccg acaaaaacca tcaactattt cgcggcgccc ccggccctcc
    28381 agcctaatcc accatcttgc ccagcttctg tggatcgaag ccatcctccc ggagcaccgc
    28441 gtcatccacc ggaatgcccg ccttggcgag gcgctcgacc agcacccgca ggcgccggtc
    28501 ggtctcggcc gcatggtcga tcatcccgtg caaggccttg accagcggat cgtccagatc
    28561 ccgcgtcacg ccgtaggccg tgaaggcctg gcgcgccggc gcgtcggcct tcacgtcagg
    28621 cgactcatcc ttgcccgcat cgacgatccg cgccggattg cccaccgccg tcgccccggc
    28681 cggtaccggc ttgatcacga cggcgttgga gccgatcttg gcgccgtccc ccacctcgaa
    28741 accgcccagc accttggcgc cggcgcccac caccacgcct cggccgaggg tcgggtggcg
    28801 cttggtaccg cggtacaggg aggtgccgcc cagggtgacg ccctgataaa tcgtgcagtc
    28861 gtcgcccact tcagccgtct cgccgatcac cacgcccatg ccgtgatcga tgaagacccg
    28921 gcggccgatg gtggcccccg gatggatctc gatgccggtc agcatgcgcc cgagatgact
    28981 gacgaagcgt cccacccagt gcaagccccg cccccatgcc gcgtgagcca cccgatggag
    29041 cagcaccgcg tgcatgccgg gatagcaggt cagcacctcc cagcgggacc gcgccgcggg
    29101 atcccgctcc aggacattgg ccacgtcttc acgcaggcga cggaacatgc agggaatctc
    29161 ctcgaaccag atcagggaat ggatggatca cccgttcggt gacgggctat atttccgaga
    29221 attttagtct acttcttttc gaacgccgtc aacatcccgc gcaggatgtt cacctcatcc
    29281 cgttccagcc ggatgcgacc gtacagccgc cgcaggcgtg gccacagacg cttggaattg
    29341 accgggttga agaagccgct ggcggtgatc gcccggtcca ggtgggcgta gaaaccctcg
    29401 atctcctcgt gggtggccgg ctcgaatgcg gtgccgggcg gctcaccggg ctccagcgcg
    29461 gccatgcgca actcgtaggc aagcacctgc acggcggccc cgaggttcag cgagccatat
    29521 tcggccgccg tggggattcg caccggcatc tggcacaggg acacttcctc gttggacagc
    29581 cccacggtct cgttgccgaa caccagggcc acgtcgccgc catgggccgc gcagacgagt
    29641 tcgccggccg cctcccgggc ccagcacatg ggcaccgcca gctcacgccg ccgcgccgtg
    29701 agggccgccg aaaacacggt gccctgcaag gcctcttcaa gggtgtcgac cacccgcgcc
    29761 gccgcaagga tgtcgtcggc gcccgccgca cgggcggtgg ccacaggatc gggaaaggac
    29821 tggggacgga cgagcaccag ctgcgacagc cccatggtct tcatcgcccg ggccgccgca
    29881 ccgatattgc cgggatgact ggtgcgggaa agaacgatac gcacgcgccc aagcgcgcct
    29941 tcacggttca tattaaaatg cagggttttc cgaatgtaag aagccggacc ggcacccgat
    30001 gcctgcgccg gcggacctcc aggacgccca tgcatcccac actgaacatc gcgatcaagg
    30061 ccgctcgccg cgccgccacc gtcatcaacc gggcttccct cgacgtggac ctgttgcgca
    30121 tcgagaccaa acagcccaac gatttcgtca ccgaggtcga ccgggccgcc gaatcggcca
    30181 tcatcgacgt actgcgcgag gcttatccga gccacgccat tctagcagaa gagtccggcg
    30241 agaccggccc ggccgaggac accgagtacc agtggatcat cgacccgctc gatggcacga
    30301 ccaacttcat ccacggcttt ccgcaatacg ccgtgtccat tgccctcgcc cgccgcggtg
    30361 tggtggaaca ggcggtcgtc ttcgatccca cccgcaatga gctgttcacc gccaccaagg
    30421 gcggcggcgc tttcctcaac gaccggcgca tccgcgtctc gaagcgcctg cgcctgcagg
    30481 acgccctggt cggcacgggt ttcccctacc gccagttcgc ccacgtggac acctacctgg
    30541 cgatcttcaa ggaactcacc cagaagagtg ccggcctgcg tcggcctggc gccgcggcgc
    30601 tggatctggc ctacgtggcc gccgggcgtc tggatgcctt ctgggaattc ggcctggcgc
    30661 cctgggacat ggcggccggc agcctgctca tctcggaggc tggcgggctg gtcagcgacc
    30721 ttgcgggcga gtccacctat ctcaccaccg gcaacgtcgt ggccggcaca cccaaggtct
    30781 tcccgcagct cctgcaactg atccaggggc actgcgaagg ccagctcacc gcctgatccc
    30841 gtcgtgcgcg ccgctgccct gctccgccgc gccgccctgg ccgtcctgct cctgacggct
    30901 ggcgcgcccg ctttcgccgg caagatcctg gtgtggggcg acagcctctc ggccggctac
    30961 gggctgcgtc cccaggaagc gtggcctgat ctgctgggcc agcggctggc gggcgaaaaa
    31021 tcggcctgga cggtcgtcaa cggcagcatc agcggcgaga ccaccgccgg tggccgggcc
    31081 cgcctgcccg cagccctgga tcgtcagcgg ccggccgtcg tgatcatcga gctgggcgcc
    31141 aacgacggcc tgcgcggact gcccgtcccg gccatggtcg ccaacctgca ggccatgatc
    31201 gacgccgccc gcaaggccgg cgcgcaggtc ctgctggtag gcatgcgcat gccgcccaat
    31261 ttcggaccgg acttcacggc ccgctacgaa aaggcctttc gcgatctcgc ccgcaccaac
    31321 acgctgcgtt tcgtgccctt tttgatggat ggttttgccg accagccggc cttcttccag
    31381 ccggacggtc ttcacccggt ggccagcgcc cagccgcgga tcctggaaac cgtctggaag
    31441 gaactcaagc ccctgctgca ctgaagaaca gcgcggcggc cttcagggca gacgcaccac
    31501 cgggttgtgc cccttcacgg cacagatgcg ctggatcacc gccggcttcg gctcgctcac
    31561 ctcgccctgc tcgtaggcca ggatcgtgca tgcaccggcc acccgctcga tcaattcgtg
    31621 ggagcgcagc aggaattccg gattgctcgg cttgccgaaa cgctcgttgc ccagcatgaa
    31681 ggtctcgtag agcagcaccc cgccggggtt gagcgctgcc agcaagagat tgaagcgcgg
    31741 ccggaacagg tagttcgtca ccaccacgcc gtcgaagcga cgatctccgt aaggccagtc
    31801 gcccgcctcc aggtccgcct cgcgggtcgt cacgtggggc accgtggcga gcagttccag
    31861 cgccaccgca tcccggtcca ccgcctcgac ccggtaaccc cgcccggcca gccagcgggc
    31921 atgccgaccg ctgccacacg ccagatccag cacctcaccc ccggccgcga tcaggggtgc
    31981 aaagcgggtg acccacgcag acggggtcag cgacgcggcg tggcgcatca tcggtccagc
    32041 ccgcccaggc acaggtattt cagttccagg tattcgtcga tgccatggcg cgagccttcg
    32101 cgccccacgc cagactgctt catgccgccg aagggtgcca cctcattgga gatcagcccg
    32161 gtattgatgc ccaccatgcc gtactccagc gcctccccca cccggaagat gcgccccacg
    32221 tcacgggcgt agaagtaggc ggcgagtccg aattcggtgt cgttggccat gtggatcgcc
    32281 tccgcttcgg tcttgaagcg gaacaggggc gccaccggcc cgaaggtttc ctcccgcgcc
    32341 acgcgcatgg ccggcgtgac gtcggccagc acagtgggtt cgtagaaggt gccgccacgc
    32401 gcatgacgcc gcccgccgac gagcacccgc gcgccctggg cgagggcgtc cgccacgtgg
    32461 gcctcgacct tgtccaccgc ggcggcatcg atcagcggac cgatcgtcac acccgcgtcc
    32521 agcccgttgc ccaccgaaaa acccttgacg gcctcggcaa gacaggcggc gaaggcgtcg
    32581 tagatgccat cctgcaccag gaagcggttg gcgcacacgc aggtctgacc ggcgttgcgg
    32641 tatttggaga ccagcgcgcc ggccaccgcc gcatccagat ccgcatcgtc aaagacgatg
    32701 aagggcgcat tgccacccag ctcaagggac agcttcttca ccgtgggcgc acactgggcc
    32761 atcagtagcc gcccgacctc ggtggagccg gtgaaggaca gcttgcgcac cacctcactg
    32821 gccgtcagcg cgccgccgat ggccaccgga tcacccgtga gcacgttgaa caccccccgc
    32881 ggaaatcccg cgcgatgggc cagttcggcc agcgcgaggg ccgtgaaggg cgtctgttcg
    32941 gcgggcttga cgatcaccgt gcaaccggcc gccagcgccg gcgccacctt gcgagtgatc
    33001 attgccgccg gaaaattcca gggcgtgatt gccgcacaga cgcccacggg ctggcggatc
    33061 accatcaggc gtcgatcggc ccccgtggtg ggaatgagct caccgtacag tcgcttggcc
    33121 tcctcggcga accactccac gaaggatgcg gcatagacca cctcgccccg cgcctcggcg
    33181 aggggcttgc cctgctccag ggtcatcatc cgcgccagat cgtcggcgtg ggtgtggatc
    33241 agctggaacc agcgctgcag gatcgcggcg cgctcccgcg cgagcaggct gcgccacgcc
    33301 ggcaatgccg cattggccgc cgcgatggcg cccgccgttt ccgtcgcccc catggcgggg
    33361 acactgccca gcgaatttcc gtcgaacgga ttgcagacct cgaggcgggc accggaatcg
    33421 gcgtcggtcc agacaccatt gatgaaggcc tgggtgcgga acagcagggg atccttgagc
    33481 gatttcatgg gcagcggagc ggacggagag acggcaccga cggtgcggaa ggctggatat
    33541 tagcgcgacg gcgccggcca cgcatgggct tgaacccatg aaaacgggcg ccgcagcgcc
    33601 cgttttccgt gcagacgatc ccgttgagac ttactgggca gcctgctcgt tcagcaacgc
    33661 cttcatggac aggcggacgc ggcccttttc gtcggtttcg agcaccttga cgcggacgac
    33721 ctgaccttcc ttgaggtagt cgctgaccgc attgacgcgc tcgttggcga tctgggacac
    33781 gtgcagcagg ccatcacgac ccggcaggat gttgatgatc gcgccgaagt cgaggatgcg
    33841 caccaccgga ccttcgtaga ccttgcccac ttccacttcg gcggtgatgt cctcgatgcg
    33901 cttcttggct tcctgggccc cttcggcgct gacggaggag atggtcacgt tgccgtcgtc
    33961 ggtgatctcg atgatggtgc cggtctcttc ctgcagcgca cggatcaccg caccgccctt
    34021 gccgatcaca tcgcggatct tttcggggtt gatcttgatg gtgagcatgc gcggcgcgaa
    34081 ggtggacacc tcgccacggg cctcgcccag ggcatccttc atcaggccga ggatgtgcag
    34141 gcggccttcc ttggcctgcg ccagggcgac ctgcatgatc tccttggtga tgccgaggat
    34201 cttgatgtcc atctgcaggg cggtgacgcc cttgtcggta ccggccacct tgaagtccat
    34261 gtcgcccaga tggtcttcgt cacccaggat gtcggtcagc acggcgaagc ggccaccgtc
    34321 cttgatgaga cccatggcga tgccggccac cggcgcggac aggggcacgc ccgcgtccat
    34381 catggacagg gagccgccac agaccgaggc catggaactg gagccgttgg attcggtgat
    34441 ctcggagacc acacgcacgg tgtaggtgaa ttcttccttc ggaggcagga cggcggccag
    34501 agcgcgcttg gcgagacggc catgaccgat ctcacgacgc ttgggagagc cgaaacggcc
    34561 gcattcaccg gtggagaacg gcgggaagtt gtagtgcagc aggaagcgct cgcggtattc
    34621 gccggccacc gcgtcgatga tctgctcgtc gcggccggtg cccagggtgg tcaccaccag
    34681 ggcctgggtc tcgccacggg taaacagggc ggaaccgtgg gtgcgcggca gcacgccgac
    34741 gcggatgtcg atggggcgca cggtgcgcgt gtcgcgcccg tcgatgcgcg cctcaccgct
    34801 gaggatgcga ccgcggacga ccttcgactc caggtcgaag aggatgttgt tgacggtgtt
    34861 ggcgtcgtag gcgcggccct ctgcggtgag ggcggcgtgc acttcatcac cgatggcgga
    34921 cagctgctgg gagcgggcct gcttggcggt aatgcggaat gcagcatcga tcttctcgcc
    34981 agcgatctcg gtgacgcggg cgatcagggc ctcgtccttg gcgggcggct gccagtccca
    35041 ttcgggcttg ccggcgactt ccaccagttc attgatggcg gtgatggcgg cctgcatctg
    35101 ggtgtggccg aacaccacac cacccagcat cacgtcttcg ggcagctcgc gggcttcgga
    35161 ttccaccatc agcacggcgg tttcggtccc cgccaccacc agatccatct ggctggtctt
    35221 gagctgggtg gcggtggggt tgaggatgta ttcgccgttg gcgtagccca cgcgacaggc
    35281 accgatcggg cccgcgaagg ggatgccggc gatggccagg gccgccgagg cgccgagcat
    35341 ggcggggatg tccgcatcca cttcgggatt gagggacatg gcctgcacga taacctgcac
    35401 ttcgttgtag aaaccatccg gaaagagcgg acggatcggg cggtcaatca ggcgggaggt
    35461 gagggtttcc ttttcggtgg gacgcccttc acgcttgaag aaaccgccgg ggatgcgccc
    35521 cgcggcgtag aacttctcga cgtagtcgac ggtgagcggg aagaaatcct gaccgggctt
    35581 ggcgttcttg gccgccacca cggtggccag gacgaccgtg tcgtccatcg tcaccatcac
    35641 cgcaccgtgc gcttgacgag cgatctcgcc ggtctcgaag gtgaccgtgt gggaaccgta
    35701 agtaaaagtc ttcttgatgg aagtaggcaa gggatgtcct ttcattcaac cggtcggcgg
    35761 cagaccacaa ccgcttcatc gaccgaggtc acgcgctgaa tttccagcgc acagatgtga
    35821 caaagccggc caagtcacca gacttgcgcc ggccatgccc atgaagaagc ctgcgcacgt
    35881 tgctgcagga caaattcacg ttcttcgcaa tgagctgctt tttccgctta cttgcgcaga
    35941 ccgaggcgag cgatcagctc acggtagccg tcggcgttct tgcgcttcag gtagtcgagg
    36001 agcttgcgac gctggctgac catctgcagc aggccgcggc gggagtggtg atccttgacg
    36061 tgagccttga agtgaccggt cagatcgttg atgcgggcgg tcagcagggc gacctggact
    36121 tcgggggagc cggtgtcgcc ctgagcgcgt tggtggtcgc gaacgatttg cgctttttgt
    36181 tcggtggaga ttgccatgaa tgaactcggt tagtttagcc ttgcgaagcg cggaattata
    36241 gcgaacgatc ccactgaagc tcaaggcttt tcattgatcc gcggtggata ccagacgcgt
    36301 cgggaccagc agcccgcctt ctgcgagggt agccaccccc aggaagcgcc ccgcgtggta
    36361 gacccgataa gcttgcccag cctcccccgc gcaaccccgc gccgcctgcc cgtgggtcag
    36421 gctgcgggcc tgatcggcgt ccaggctgac acacggcatg tggaccaaca gtgcatcgac
    36481 cggagccagg gccgcatccc gtgccgccgg tgcacacgcg tcgaaatcag ccagcggaat
    36541 cgcggcatcc aatgcaaacg gcccgatgcg ggtccgccgc agggcggtca ggtgggcgcc
    36601 gcagcccagc agggcaccca gatcggatgc cagggtgcgg atataggtgc ctttgctgca
    36661 gtccacgcgg atgcgcaggg cgtcacccgc acgttccagc aactcaaggc gatggatcgt
    36721 cacccggcgc ggcgcgcggg gaatctccac cccttcgcgg gcgtattcgt agagggcctt
    36781 gccgtggtgc ttgagggcgg aatacatggg cggcacctgc tcgatttcgc cccgcaactg
    36841 cgccagggcc gcggccagct gcgcgtcgga tacgtgcacc gggcgtgtgt cgagcacctc
    36901 gccttcggca tcggcggtgg tcgtggtgac gccgaggagg acggtggcgt cgtaggcctt
    36961 gtccgcgtcc agcagggtct gggagaactt ggtcgcttcg ccgaaggtca ggggcagcag
    37021 gcccgtcgcc atcggatcca gcgtgccggt gtgtccaccc ttggccgcat tgagcagacg
    37081 ccgcgcctgc tgcagggcat ggttggaact gagcccctga ggcttgtcga gaaacaggac
    37141 gccatccacg atccggcgcg ggacacggcg tggcggagga gcttggacca cgggaaaacc
    37201 ttcgcagcgg gcggccgcgc cgccccgcaa tcaatcgacg gacgggccct tgccagacgt
    37261 gtcgtcgccg gcggcttcat cctcgggatg gaactgccga tccgactcca gggcccggtc
    37321 gatgagggac gacaggcggt tgccgttctc caccgagggg tcgtactgga agtgcagctc
    37381 cggcagggtg tggatgcgca cccggcgccc cagttcgcgg cgcaggaagc cgctcgcccg
    37441 gcgcagcccg cgcaggatgt cgtcgagccc atcctgaccg gtcatggacg tgaaatacac
    37501 ctttgcgtgg gcgtagtccg gggtgagctc cacgtcggtc agggtgatga agccgacgcg
    37561 ggggtccttc acttccaggc ggatgagctc ggccagttcg cgacggatct gctccgccac
    37621 acgctggcca cgggaaaact ccttgggcat cgcttacagg gtccgggcca cttcctggat
    37681 ctcgtagatt tcgagctgat cgccttccac gagatcgttg aaattgcgga tctgcagacc
    37741 gcactcatag ccggccttga cttccttgac atcgtccttg aagcgcttga gggattccag
    37801 ctccccggta tggatgacca cgtggttgcg cagcacgcga acctgggcat tccgcttgac
    37861 cagaccttcc agcacgtaac acccggcaat ggttccgacc ttggagatgg taaagacctg
    37921 acggatctcc accatgccca gcacctgctc gcgcttctcg ggggccagca tgccggacag
    37981 ggcgctcttc acctcatcca cggcgtcgta gatgatgttg tagtagcgga tgtccacgcc
    38041 gaagttctcg gccagcttgc gggcgttggc atcggcccgg atattgaagc cgatgaccac
    38101 tgcgccggaa gcctgggcca ggttgacgtc ggattcgctg atcgcgccca cgccaccgtg
    38161 gatgacctgc acgcggactt cctcgttgga gagcttgacc agggcatggg ccagggcttc
    38221 ctgggaaccc tgcacgtcgg ccttgatgat gaggggtagg gacttgacct cgccctcgcc
    38281 catctgctcg aacatgctct ccagcttggc ggcctgctgc ttggcgagct tgacgtcccg
    38341 gaacttgccc tgacggaaca gggcgatttc ccgcgccttg cgctcgtcgg ccagcgccac
    38401 gacctcgtcc ccggcgcccg gcacttcgga cagaccaagg atttccaccg gaatcgacgg
    38461 accggcttct tccaccggct tgccgttctc gtcgagcatg gcgcggacac ggccgaaggt
    38521 ggcgccggcg aggatcacgt cgccacgctt gagggtgccg gactggacca gcagggtcgc
    38581 caccgggcca cggcccttgt cgaggcgcgc ttcgatgatc aggcccttgg ccatggtgtc
    38641 gtggggcgcc ttgagttcca gcacttcggc ctggagcagg atggcctcga gcagatcgtc
    38701 gatgccctga ccggtcttgg cggacacctc gatgaacatg gtgtcgccac cataggcttc
    38761 cggcaccacg ccttcggcga tgagctcctg acgcacccgc tcggggttcg cgcccggctt
    38821 gtcgatcttg ttgaccgcca ccaccagcgg cacgccggcc gccttggcgt ggtggatggc
    38881 ttccttggtc tggggcatca cgccgtcgtc ggccgccacc accaggatga cgaggtcggt
    38941 ggccttggca ccgcgggcac gcatcgccgt gaaggcctcg tgacccggcg tgtcgaggaa
    39001 ggtgaccgtg ccacgatcgg tatccacgtg atacgcgccg atgtgctggg tgatgccacc
    39061 cgcttcaccc gccgccacct tggcgcgacg gatgtagtcc agcagcgagg tcttgccgtg
    39121 atccacgtga cccatcacgg tcaccaccgg agcacgggac tcttccacca cgtccttgtg
    39181 ttcggcattt tcctcgagga aggcgtccgg atcgtccagc ttggcagcaa gcgccttgtg
    39241 gcccatctcc tcgaccacga tcatggccgt gtcctggtcc agcacctggt tgatggtgac
    39301 catggatccc atcttcatga gggtcttgat gacttcggtg gccttcaccg ccatcttgtg
    39361 ggccagatcg gcaacggaga tcgtctccgg cacgtgcacc tcacggatga tgggttcggt
    39421 cggcgcgacg aaggccggcg cctcatggtg acgtccgccg gaacgaccac cgcgggcacc
    39481 cttccagccg cccgcaccgc ccgctgcatc gccgcgagtc ttcagaccgc cgcgacgctt
    39541 tgcgtcgccg gctgcttcct tggcgaccgg cgtgcccttc ttgacgtcct tgcgggcacc
    39601 cttgtcatcg gccttggcgg gcttgtgaag cgtgccactc ttggcacccg cctcggcatc
    39661 ctgacgactc ttgtcgcctt cggactgccg ctgcttctcg tcttccatgc gctgcagcat
    39721 cgctgcacgg gcgcgggctt cgcgttcctg cttttccttg agatcggcag cctgacgggc
    39781 ggccagctca gcctgacgct gcgcctcgcg ctcgcgggac gcgcgctctt cggcagagat
    39841 cacggaactc acgacgcgac gcaccggccc ttgctgggga cggggctcag gcttggccgc
    39901 gggggccacg gcgacgggtt cgggcacgac ggcgaccggt tcaggctcgg gctcgggggc
    39961 gaccgtttct tcgggtgcag ccggctccac aacgggctcg ggctcgggtt cgacttcgac
    40021 gaccacctcg accggcgcag cctggacagg ttcgggcgca gccatctcga tgggttcggg
    40081 aaccggagtc ggctcgagca cgggttcggg caactcggca ggcatttcgg gaacgagcgc
    40141 ggcgcccggc gccgcagctt cgggcaccag caattcgtca cgcttgacga agacacgctt
    40201 cttgcgcact tcgacctgca ccgtgcgggc acggccggtg gagtcggtcg ccttgatctc
    40261 gctggtctgc ttgcgcgtca gggtgatctt ggcctttgcg gcggaatcac catgggcgcg
    40321 gcgcaggaaa tccagcagac gggtcttgtc ctgctcggtc agcagatcgg tttcccgcga
    40381 cttgtccaca cctgccttct gcaactgttc gagcagcacg gaggccggca ttttcagttc
    40441 gccggcgaat tgcgtaacgc ttgtctgtcc catcttgccc tccgggtatt actcgaacca
    40501 gtgcgcgcgc gccctggtaa tcagattctt ggcccgctct tcgtcgaggc ccgacatgtc
    40561 catcaactcg tctaccgcca gatcggccag gtcgtcgcgg ttatggacgc cagcctgggc
    40621 cagcttggcg gcgagcggct tgtccatgcc atccaggctg agcagatcct cggacacggt
    40681 ctcgagctgc tcctcggtga cgatggcttc ggtgaggagc acgttgcgcg cgcggttgcg
    40741 cagctcgttc accgtttcct cgtcgaagga ttcgatctcc agcatctcag ccaggggcac
    40801 gtaggcgatc tcctccaggg acgagaagcc ctcgtcgatg aggatgttgg cgacttcctc
    40861 gtccacatcc agcttgctca tgaagatctg acgcacggaa ctgtgctctt cctcggactt
    40921 gcgctccgac tcggcctcgg tcatcaggtt gatggtccag ccggtcagct cggacgcgag
    40981 cttcacgttc tggccgttgc ggccgatggc gatggccagg ttgttctcgt ccaccaccac
    41041 atccatcgca tgggcttctt cgtccacgac aatggacgac acttccgccg gctgcagggc
    41101 gccaatgacg aactgggcgg gctcgggaga ccacagcacg atgtcgatgt tctcgccgcc
    41161 gatctcgttg cgcacggcgg tgacgcggga accccgcagg ccgacgcagg taccgatcgg
    41221 gtcgatgcgc ggatcgttgg actttacgcc gatcttggcg cgcagaccgg ggtcgcgcgc
    41281 acaggccttg atttcaagca aaccgtcgtc gatctcgggc acttccagct cgaacaggcg
    41341 gatgacgaac tccggcgcgg tacgcgacag gatcagctgg gggccgcggg caccgcggtc
    41401 gatgcgcagc aggtaggcct tgacccggtc gccgacgcgc aggttttcct tggggatcat
    41461 ctggtcgcgg ggcagcaccg cttcaaggcg gcccacttcg atgatcgcat tgccccgctc
    41521 catgcgcttg atgctgccgg agatgatgaa ttccttgcgc tcgaggaagt cgttgaggat
    41581 ctgctcgcgc tccgcgtcac gcaccttctg gaggatcacc tgcttggcag cctgggcgcc
    41641 gatgcggccg aagtcgatgg gctcgaggga ttcctcgacg aaatcgccca cctggatctc
    41701 ggggtattcc tcgcgggcat cgatcagccc catctgggct tcgtcgttgt ccacttcctc
    41761 gtcgggcagc acctgccagc gacggaagga ttcatagtcg ccggtttccc ggtcgatgga
    41821 cacgcgcaca tcggcttcgt cgtggatgcg cttcttggtg gcctgggcca gggcggactc
    41881 cagtgcggag aagacgatct ccttggacac gttcttctcg cgggccaggg cgtcaaccaa
    41941 cagcaaaatt tcacggctca taccggcaaa cctccaaatt ccttaaaacc tgggcaccag
    42001 gcgggccttg ttgatctctt cgaacgggat cgcgagggat cccttttccg tttccagcgt
    42061 caccacaccg tccgtgagtc cgaccagggt gccggtgaaa ttgcgctggt tgtcgatggg
    42121 caggttcagg cggacctgga tcgtctcgcc ggcgaagcgt tcgaaatccg tcgccttctt
    42181 gagcacgcgg tcgagaccgg gggacgatac ttccagccgg tcgtaatcca cgttctccac
    42241 ctcgaacacg cggcagagct gattgctgac cgtcgcgcaa tcgtcgaccg tgataccgtc
    42301 cggcgcgtcg atgaacacgc gcagcaggcg cccacggggg gacgattcca cgtccaccac
    42361 ttcgtagccg agcccttccg ccgtctgttc gatcagttgt tgcagatcca tgccttggcc
    42421 cacaaattcc tagcccacaa atgaaaaatg ggcgaaacgc ccatccctgt tgaattcgac
    42481 cggatgtccg gcccttgata ctgcttttcg ggccggtgaa acacgaagag tataccagcc
    42541 cgccgccgca ggcgcaattt cgggcccgtt cagccttttg gcggggtacg ccgcggcgtg
    42601 ctgcggggcg gcttgcgggg ccccggcgcg cggatcggct tgggcaaacc ggccagggcg
    42661 cagacgtctt cctcgggcat gtcttcccac atgccgcgct tgaggcgcgt cggcagactg
    42721 atgggaccgt agcgcacccg catcaggcgg ctcacggtca gaccgagcgc ctcgaacatg
    42781 cgccgcactt cccggttgcg cccttccgac aggctcaccc ggtaccagtg attgacccct
    42841 tcgccacccg catcgagcag ggtgttgaag cgcgccggcc cgtcgtccag ctcgatgccg
    42901 tccagcagcg cctgcttctg cacttcgtcc agttcgccca ggagacgcac ggcgtattcc
    42961 cggtccagct cgtagcgcgg gtgcatcagg cggttggcca gatcgccgtc gttcacgaag
    43021 agcagcaggc cggaggtgtt gaagtccagc cgtcctaccg cgatccagcg ccccttgcgc
    43081 agcatgggca gacggtcgaa cacggtcggg cgaccctcag ggtcgtcccg cgagacgatc
    43141 tcgccttcgg gcttgtgata cagcaacacc cgcgggacgc ggggctggaa gcggatcggc
    43201 atgagcttgc cattcacccg cacccggtcg ccggggccga ccttctggcc cacgtcagcg
    43261 ggctcaccgt tgacggagat gcggccggcg acgatccact cttcgatctc gcgccgggag
    43321 ccgaggcccg ctgccgcgag caccttgtgc aggcgctggg gctcggtgta ggcggcgtca
    43381 cggccctgcg gacggcgctg ttcacgctca ggcgtcgctg aagtcggcag cgcgtcgccg
    43441 gaggtgggca gtccgtaggc tgccgccccg ctctgcgcct tgcgggcacg ccagttgccg
    43501 cggcccccgg tgtcgccgag gccgatggcc gaggcgttgc tgcggggctg gcgggcacct
    43561 tcgtcctggt cgccccggga cgcctcggcg gacccacggc cacgtccgcg cggcggacct
    43621 ccggcagatg tgccgcggat cttgccaccg ggcgcgggcg cgccggtctt gggcctaaac
    43681 ggccggttct tcggtttttg ggagttcgac aagatccatg atcctttcga tttcggtcag
    43741 cggaggcaat tcggtcaggc tgcgcagacc gagatcctcc aggaatttgc gtgtcgtggc
    43801 atacaggccg gggcggcccg gcgtgtcccg gtgtccgacc acatccaccc agccccgcga
    43861 ttcgagggtc ttgaggacgc tgggcgacac catcacgcca cggatgtcct cgatgtcacc
    43921 ccgcgtcacc ggctggcggt aggcgatgat ggccagggtt tccaacaccg cgcgggaata
    43981 cttgggcggt cgttcgttct tcaggcggtc gaggtagatc tggtactcgg gccgggtctg
    44041 gaagcgccag ccgctggcgg tctgcaccag ctcgaccccc ctgccctgcc agtcgcccgc
    44101 cagctcgacc agcaggcgcc gcaccaggtc gccgcccgga tcgtcctcaa agagccgacg
    44161 cagctcgctc acctgcagcg gtgccggggc agccagcagg gcggtttcga gcacgcgctt
    44221 gaattcgtca ggcgtctgcg gcggaggcat cgctgcgttt cacgtagatc ggtgcgaagg
    44281 cctcgttctg ggtcaccacc accaggcgct ccttgaccag ctccagggtg gcgagaaaga
    44341 ccaccaccag gccggccacc ccgagtgccg gatcgaacag ggattcgaac acgacgaagg
    44401 cgtcgccgct cagcttgcgc aggatcaggc tcatgtgctc gcgcaccgac agctcctcgc
    44461 gctggatcgt gtggtgccgg cgcaggcggt tctggcgggc gatcttgagc caggccagtt
    44521 gcaggtcgtg gaggctgacg gtgggcagac gctccaccac cttctcggcg acaaacacgc
    44581 cgacccattc gaaatcccgc tcggcacggg gcaatgcgtc cacccgcgcg gcggcgagct
    44641 tgatctgctc gtattccagc agacgccgca ccagctcggc ccgcgggtcg agcacctcct
    44701 cgccgtcatc ccgcggcggc cgcggcagca gcatgcgcga cttgatctcg aggagggtcg
    44761 cggccatcag caggtaatcg gcggccagtt ccaggttgtg ccggcgcatc gcctccacgt
    44821 attccaggta ctgggccgtc aacggcacca tggggatgtc gaggacgttg atgttggcct
    44881 tacggatcag gtagagcagc aggtcgaggg gaccttcgaa ggcgtcgagg aagacctcca
    44941 gcgcttcggg cgggatgtac agatcgcgcg gcagctcgaa gatcggctgg ccgtacatgc
    45001 ggccaatcgc gctctccggc gcttcggccg gcggcgccag gtcgagcacg gcgttggcgg
    45061 aggcgctgtc catcggcgaa ccgttcaagg gcgcagcccg tcaccggccg cgccttgcag
    45121 cgcacaccgg tcgcccgacg tcgtccccgc ccgcccgctc acgagtagct gagccccatg
    45181 gattcgcgca cgtcgcgcat ggtttcctgg gccagcttgc gggccttgtc acagccgtcg
    45241 gcgatgatgc tgcgcaccag ggtgggatcg tccaggtagg gctgggcgcg ctcgcgcatg
    45301 gcctcctgtt ccttgagcac cgcatcgacc accggctgct tgcactccag gcagccgatg
    45361 ccggcgctct tgcagccacg gtccacccat tcccgggtgg gcgcgtcgga atagatgacg
    45421 tggaactgcc acaccgggca cttttccgga tcgcccggat cggtgcgtcg cacccgcgcc
    45481 ggatcggtct gcatggtgcg gatcttcttg gtcagggcat ccgcctcctc ccgcaggaag
    45541 atggtgttgc cgtaggactt ggacatcttc tggccgtcca ggccgggcat gcgcgaggct
    45601 tcggtgagca gcgcgtccgg ctccacgagg atcatcttgc cactgccttc caggtagccg
    45661 tagagacgct cccggtcgcc gtgggagagg ttctgggctt cgtcgagcag cgccttgccg
    45721 agggcgaggc cttcgtcgtc accgttctgc tggaagcggg tgcggaactc ctcgtagagc
    45781 ttggcgcgct tggagccgag cttcttgacg gcctctctgg ccttgtcctc gaagccgggc
    45841 tcgcggccgt acatgtggtt gaagcggcgg gcaagctccc gggtgaattc cacatggggc
    45901 agctggtctt cgcccacggg caccttgtcg gcccggtaga tgaggatgtc cgcgctcatc
    45961 agcagcggat agccgaggaa gccgtaggtg gacagatcct tattgacgag cttttcctgc
    46021 tggtccttgt aggtgggcac ccgctccagc cagcccagcg gcgtcatcat ggacatgagc
    46081 aggtgcagct cggcgtgctc gggcacccgc gactggatga agatggtggc ctgagacgga
    46141 tcgacgcccg cggcaagcca gtccaccacc atgtcccata cgctgttctc gatgcccttg
    46201 gggttgtcgt aatcggtggt gagggcgtgc cagtcggcca cgaagaacag gcagggatac
    46261 tcggcctgca gcttcaccca gtttttcagg acgccgtggt agtggccgag gtggagacgg
    46321 ccggtggggc gcatgccgga gaggacgcgt tcagcgaaca tgggaagtca aagtccaaaa
    46381 acggttgcaa gggcaaatcg gaaaagagcc atcaggggat ccaggatgtc tccgaggata
    46441 ccggtaaaca gcagcaggag caggataggg aagccgtagg gttcgatctg cgcgaacttc
    46501 cacgccgggc cggccggcag caggctcact acgatgcgcc cgccatccag cggcggcagg
    46561 ggcaacaggt tgaggagcat cagcacggca ttgatctgca cgcccgcgtc agccatcttc
    46621 agcaagggca gcgtgtaggc gttttcgggc aggctgaagc ccagtttgag caccaccatc
    46681 cacccgatcg ccatgcacag gttggccagc ggtccggccg ccgccaccca cagcatgtca
    46741 gccttggggt ggcgcaagcg gccaaagttg accggcaccg gcttcgccca gccgaacagc
    46801 atcggcgaac cgcccaccag ggcgctgctt gcgaggatca cgccagggat cagcaaggtg
    46861 cccaccgggt cgatgtgacg cagcggattg aggctgatgc gcccggccag ctcggccgtc
    46921 cggtcgccga aatggcgcgc cgcgtagccg tgggccgcct cgtgcagagt aatggcgaac
    46981 agcacgggca aggcccagat ggccagcgag gagatgagtt cttgcatgga ggacggattc
    47041 gcgtcgggcg ccgattctag cagagcctgc gccccgaccg cggccgagct gccggacgcc
    47101 tgctcacaga ccgaagggcg cgaggctgcc gcgcccttcc cgcaccagct caggggtacc
    47161 cgaggtgagg tcgatcaccg tggtcgcctc gccgcggcag gggccggcct cgatgaccag
    47221 gtcgacggat ttctccaggc ggtcgcggat ctcctctgcg tcggtgaggg gatgttcctc
    47281 acccggcaac agcagcgtgg agctcaacaa cggctcgtcc aactcggcca gcaaggcggc
    47341 cacgagggga tgatcgggca cccgcaggcc gatggtcttg cgcttgggat ggagcacccg
    47401 ccgcggcaac tccttggtac cttcgaggat gaaagtgtag gggccgggcg tggtcgcctt
    47461 gagcagtcgg tactgggcgt tatccacccg cgcgtaggtg gcgatctcgg agaggtcgcg
    47521 acagatcagc gtgaagtgat gccgctcgtc cacgccgcgg atgcgccgga tgcgggagag
    47581 gagttcgtta tcgccggtat gcccgccgag ggcgtaggcg gaatccgtcg ggaacgccac
    47641 gaggccgccc tcgcgcatga tctgcgcagc ctgacggatc aggcggggct ggggatgttc
    47701 gggatgaaga ctgaaaaact gggccatgac acagattgca agaagaggag gctcactgca
    47761 ggcgcgtcca caccggcgtg agatcgggcg gcaggggcgg cgcccggccg aggtcgaccg
    47821 gactgtcttc cggtccatga aaatccgagg cacgggacgc cagcagacca tacttgcggg
    47881 caactcgcga aaaggccagc acctgccccc catcgtgggc gccgcagcag acctccacac
    47941 cctcgccgcc caggtcgcgg aagcgctcga agagcagatc catctccacg tgaccgagac
    48001 gatagcggcc cggatgggcg atcaccgcga caccgcccgc accacggatc cagccgaccg
    48061 cgtcatccag ggtcgcccag acgtgctcca cgtagccggg tttgccccgc gcgaggtaat
    48121 ggaggaagac gtcgtggacg ttgcgtgcga tgccgatctc cacaagatag cgtgcgaaat
    48181 gcgcccggct gaccagggcc ggattgccgg cgtagcgcaa ggcgccttcg agggcaccat
    48241 ggatgccgat ccggtcgagt tccgcggcga tctgccccgc tcgaccgtcc cgcccttcgc
    48301 gcacttggtg cagaccggcc accagcgccg gatggctcgg atcgatattc aggccaacaa
    48361 catgcaccgt ctggtcgagc caggaaacgg agacctcgac cccaggcaca aaacgcaggc
    48421 cgacgtcccg ggccgcgtcc gcagcctcgg caaggccaag cagttcgtca tggtcggtga
    48481 gggcgaggag gtccaccccg ttggcgtggg cacggcgcac cacctgggcc ggcgccaggg
    48541 tgccgtcgga ggcggtggaa tggcagtgca gatcggcgtt gactgggttc atgatgcctt
    48601 cgacaacagg aacgtctcga tgatccgggc cacttccccc ggctggtcgt gatgcaggtt
    48661 gtggccactg tccgccacgg tcacttcgcg aaaatcccgg aagcagtcac gcgcctcggc
    48721 gtgcgcctcg tcgctgaccc ccaggcggcc acgcagcgcc gccgcttccg gcaccaccca
    48781 cagggtgggc gccgtgaccc gctgccagca ggcgcgggcc tccccacgcc ggtacagcac
    48841 cggattcacg cgccggtgag ccggatcgcc caccagacgg atgccgccct caccgtccgc
    48901 ctcgcccaga tgctcggcaa gccacgcagc ccgctcggcg ctcagacgcg gattgtccgc
    48961 ccgcaagcgc tcggccagcg ccgcgcagtc cggatagctg cggaaggcca ccggcgcgga
    49021 caattcccgc agccattttt ccagtcggcc gggcgcctgc tccggcgccg tgtcggcaag
    49081 gccgaaggcg tccagcgcca ccagccgccg cacccgcgcg ggccggacac ccgcgtacag
    49141 gcaggccaca ttgccgccca gactgtggcc gaccagatcg accggtgcat ccggcgacaa
    49201 ggccgtcaac aaagcatcga gatcgccgag ataatcggga aaccagtagg catcgccccg
    49261 ccactcggag tgcccgaagc cgcgccagtc cggcgccagc acgtgccagt cgtgccgcag
    49321 ggcttccacg acgaactgaa aggacgcgga cacgtccatc cagccgtgca gcaggaagag
    49381 ccggggcgca ccttcgcgcc cccactcccg caggtgatag cgcaggcccc tcacgggcag
    49441 gaagcggctt cgcgacgtcc acatccatgg cattgtaaca gacgcttgcc gcccaccgcc
    49501 cgcaatggta tggtgcgcgc cgtcgtacgg gagagcgttc gcacccgcgc tgcgaaccgc
    49561 cgaaggcgca acccccggaa ccgctcaggc aaaaggaccg acgacgaatc acaaatctgg
    49621 agagtgatgc cgcccgccac gccgggtccg catccaccga aggggcacgc agcgtgtcgg
    49681 acacgccgca atctctcagg tacaagggac agatggggcc gccgcgatgg cattgtcgcg
    49741 ccggcccttt tgtttgccta aactgaaccg attcgccacc ggagagctcc atgccccagc
    49801 acacccccct ctacgccacc cacgtcgccg ccggcgcccg catggtcgat ttcgccggct
    49861 gggagatgcc cgtcaattac ggctcgcaga tcgaggagca ccatgccgtg cgtcgcaccg
    49921 ccggcatgtt cgacgtctcc cacatgctcg cccttgacct ggacggacgt gacgcccggg
    49981 gcttcctgcg cggcctgatc gccaatgatg tggccaagct cagcgaaccg ggcaaggcct
    50041 tgtacgcctg catgctcaac ccggacggcg gcgtcatcga cgacctgatc gtctaccacc
    50101 tgagcgacac ccattaccgc gtcgtcgtca acgccggcac cgccgacaag gacgtggcct
    50161 ggatgcacga gcgcctgcag gcttccggcg ccgacgcccg cctcaccgtg cgccgcgacc
    50221 tggccatgat cgccgttcag gggcctgagg cccgtgccaa ggtgtgggct gccattcccg
    50281 gcgcggaggt cgtctcggcg cacctcaagc ccttccaggc ggtcgacttc ggcgagtatt
    50341 tcatcgcccg cacgggctac accggcgagg atggcttcga gatcaccctg cccgccgaaa
    50401 aagcggccgg cctgtgggtc gatctggtca atgccggcgt ggcaccctgc ggcctcggtg
    50461 cccgcgacac cctgcgcctg gaggccggca tggcgcttta cggcaacgac atggacgaga
    50521 acgtctcgcc cctcgatgcg ggtctcgcct ggaccgtgga cctcaaggac gccgcccgcg
    50581 atttcgtcgg caaggccgcc ctcatcgccc agggcacgcg ccgccagacc ctgggcctgg
    50641 tgctggaaga caagggcgtg ctgcgcagcc accaggccgt cttctgcgcc cagggcgacg
    50701 gcgagaccac cagcggcacg ttctcgccga ccctgcagaa atccatcgcc ctggcccgcc
    50761 tgcccctggg tgtccagcct ggcgacgccg tcgaggtgga catccgcggc aagcgcctca
    50821 aggcccgcgt ggtcaagccg ccgttcgcac gcaacggcca ggccctgatc tgatctcacc
    50881 ctgctccctt tctccccttt gactccccaa aaggattgaa caccatgaac acccctgacg
    50941 acctgcgcta caccgcctcc cacgaatggg tccgcaccga agcggacggc accctcacgg
    51001 tcggcatcac cgaccacgct caggaagccc tcggcgacgt ggtcttcctc gaattgccgg
    51061 atgccggccg cagcgtggag gccggcgagg ccatcgccgt gatcgaatcg gtcaaggccg
    51121 cctccgacat ctatgcgccg gtcgccggcg agatcatcgc cgccaaccag gacgccgtgg
    51181 acgcgcccga atccgtcaat gcggacgcct acgccgcctg gctgttcaag atcaagcccg
    51241 ccgccggggc ctccctcgac agcctcctcg acgccgccgg ctacgccgcc accctcggct
    51301 gactgcctgt cgctccgggc gcacgtccgt gcgcccgccc gatccttcga cgcgagtttt
    51361 ccccatgccg cagaacctcc tcagcagccc cctgtccgcc ctcgaacaac gggacgcctt
    51421 catcgaccgt cacctcggcc cctgtgcggt cgaacgcccg cccatgctcg ccgccctggg
    51481 cgtggacagc gtggagacgc tgattgccca gaccgtgccg gccggcatcc gcctggccgc
    51541 gcccctggcc ctcggcgagc cggtgcccga gcatgaagcc ctggccgagc tcaaggccat
    51601 cgcgtcgaag aatgtgctga agaaatccct gattggcatg ggctactacg acaccctgac
    51661 gcccaaggtg atcctgcgca atgtgatgga gaaccccggc tggtacaccg cctacacgcc
    51721 ctaccaggcc gagatcgccc agggccgcct ggaagccctg ctcaacttcc agcagatggt
    51781 gatcgacctc accggcctgg aactcgccaa cgcctcgctg ctggacgaag cgactgccgc
    51841 cgccgaggcc atgaccatgg cgcgccgcat cgccaaggtg aagagccagg ccttcttcgt
    51901 ggatgaagcc gtctttccgc aaacccttga cgtggtgcgc acccgcgccg agtacttcgg
    51961 cttcgagctg atcgtcgggc ccgccgagga cgccccccat cacgaggtct tcggcgcgct
    52021 cctccagtac cccaacgaac gcggtgaagt cgtcgacccg agcgccgcga tcgccggcct
    52081 caaggccaag ggcgcagtgg tcgcggttgc ctctgacctg atggccctgg tgctgctcaa
    52141 gtcgcccggc gccctgggcg ccgacatcgc cctcggctcg gcccagcgct tcggcgtgcc
    52201 cctcggcttc ggcgggcccc acgcggcctt ctttgccacc cgccaggccc acgtgcgcgc
    52261 catgcccggc cgcatcatcg gcgtgtccaa ggacgcccgt ggcaagaccg ccctgcgcat
    52321 gagcctgcag acccgcgagc agcacatccg tcgcgagaag gccaactcca acatctgcac
    52381 gtcccaggtg ctgctcgcca acatggccgg gatgtacgcc gtgtaccacg gcccgcaggg
    52441 cctgcgcacc atcgccgcgc gcatccaccg cctcgccgcg gtgctggccg aaggcctgcg
    52501 gagcgctgcg tacacgcttc gcaccgacgc cttcttcgac acacttcagc tcctcaccgg
    52561 cgcgcgcacc gacgccctgt acgccgccgc ccaggccgcc ggattcaacc tgcgccacct
    52621 ggacggtcac ggcctcggca tctcggtgga tgaaaagacc accgccgccg acgtcgccgc
    52681 cctgctcggc gccttgggcg ccccggccga catcgccgcc ctggacgccg ccgtggcggc
    52741 tcgcggcggc aatctgccca ccggtctgct gcgcgacgac gccatcctga gccaccccgt
    52801 gttcaacacc caccacaccg aacacgagat gctgcgctac ctcaagcgcc tgcagaaccg
    52861 cgacctggca cttgaccact ccatgatctc cctgggctcg tgcaccatga agctcaacgc
    52921 caccagcgag atgatcccgg tcacctggcc cgaatttgcc gatatccatc ccttcgcacc
    52981 ggccgagcag accgccggct accgggagct cctcgacggc ctcgccgccc agctgcgtgc
    53041 catcaccggt ttcgacgcca tctgcctgca gcccaactcg ggcgcccagg gtgaatacgc
    53101 cggcctcgtg gcgattcgcc gctaccatgc ggccaatggc caaggccagc gcaacgtgtg
    53161 cctgatcccc cgctctgccc acggcaccaa cccggccacc gcgcagatgt gcggcctgga
    53221 ggtcgtcgtc gtggattgcg acgacaacgg caacgtggat ctgcccgacc tggaggccaa
    53281 ggccgcccgc tttgccgatc gtcttgccgc gctgatgatc acctacccgt ccacccacgg
    53341 cgtgttcgag gagaacatcc gcgaaatctg cgccgccgtg catcgccacg ggggccaggt
    53401 gtatatggac ggcgccaacc tcaacgccca ggtcggcctc acctcccccg ccaccatcgg
    53461 tgcggacgtg agccacatga acctgcacaa gaccttctgc atcccccatg gcggcggcgg
    53521 gccgggcatg ggccccatcg gcctcaaggc gcatctggcg ccctacatgg ccgaccacgc
    53581 agtggcgccc acgggcgacg aggcacgccc gcaggccggc cagggcgccg tctccgcggc
    53641 cccgttcggc tcggccagca tcctcccgat ctcgtggatg tacatcacca tgatgggcgg
    53701 ccgcggtctg cggcaggcca ccgaaatcgc catcctcaac gccaactacg tggccaccct
    53761 gctgaaggat cactatcccg tgctctactc gggcggtcag ggccgcgtcg cccacgaatg
    53821 catcctcgac atccgcccga tcaaggccgc caccggcatc accgaggtgg acatcgccaa
    53881 gcgtctgatg gattacggct ttcacgcgcc gaccatgtcc ttcccggtag ccggcacgat
    53941 catggtggag ccgaccgaat ccgaggacaa gggcgaactc gaccgcttca ttgcggccat
    54001 gatcagcatc cgtgcagaga tccacgccgt cgaggacggt cgcctgcccg ccgacgacaa
    54061 tcccctcaag cacgcacccc atacccaggc cgatctcctg ggcgactgga accgccccta
    54121 tagccgcagc gaagccgcct tcccgctgcc ctgggtcggc gagaacaagt tctggcccag
    54181 cgtcaatcgc atcgacgatg tgtatggcga ccgcaacctg ttctgtgcct gcgtgccgat
    54241 ggacgcctac gccgactgat cgctcagcct gcccgatcac cccggttcgc cgcgtggacg
    54301 cggtggccgg ggtttttcat ttatgccgcc cgacgtgaca tatggtgaca ggcagaatcc
    54361 gcagggcagc tagaatccgg agcacttttc cgtaccgacc aggcaacttc cggccgcccc
    54421 ccggccccgc cagaggaatc cgcatgccac cgctccgccc gccccatcac ctgatggcgc
    54481 cccttgcgcg caccgccctc gccctgggca tcgccctcct gggcaccagc gcgcaggccg
    54541 acaccgatgc cctgagcagc ctcaaacaga tgagccttga cgaattggcc gacattcggg
    54601 tgtcgatcgc cagtcagcgc agcgaacggg tcgaagacac ggccgcagcg gtcaccgtgc
    54661 tcaccgccga ggacatccgc cgctcagggg ccacgacgat cccggaactc ctgcgtacgg
    54721 tgcccggcct caacgtcgcc cgtatctcgg caaccgagtg ggccgtcagt gcccgcggct
    54781 tcaacaatca gtttgccaac aagttgctgg tcatggtgga cggccgcagc gtctacacgc
    54841 cctttttctc gggcgtgctg tgggacgaac tgaatctggt gatgcaggac atcgaccgta
    54901 tcgaggtggt ccgcggcccc ggcggcagca cttggggggc caatgcggtc aatggcgtga
    54961 tcaacgtcat cacccgccac agcgaagaca cccaaggcac gctggtgggc acggttgtcg
    55021 gcaaccacca gtccgaagtc gtcgcccgcc acggctaccg cctgaacgac cacagtttcg
    55081 ggcgcgtgta cgccaaggtg catcaggccg accggtttgc caaccccgac ccgtcgaccc
    55141 aggacgacat gcgctggagc ggtggacgcg ccggcttccg caacgactgg gaatcggcag
    55201 gccacatggt cacggttcag ggcgaggcct tccgtgagat gaagcccgac gaacgacagt
    55261 attccggtgc ccatctgctc ggccgttggg aacatgggca cggggatggc agccgggacg
    55321 aattgcgggc gtactacaac cagttcagtc tcgaccactg gcgtgcggcc ggcaacgcag
    55381 ccacggccca gatcaatacg gccgacgtga acttccgcca tcattttgcc gacgccgggc
    55441 cccatagcct gattgcgggc ttcggctacc gagccgtcca ggcagatgcc aacctgaccc
    55501 tccccaccag cgtgacgcgc ccacagcgca ccgaccaatt gttcagcatc ttcgccgaag
    55561 atacggtcag cctggccgag cgtctgcgcc tgaccctcgg ggtcaagggc gagcacaatg
    55621 acttcaccgg cctggagtgg cagccgtcgg cccgcctgcg gtggacgtca ggctccggca
    55681 gcacggcctg gatggcggta tcacgggccg tccggacacc ctccatcatc gaagacagcg
    55741 caacgctcaa ctccagtctg gcccccacgc cccagacatt cggactgccc gtggcggtca
    55801 gcgccgtggg taatccgaat ctcacctcgg aacgcctcat cgcgtacgaa atcggctacc
    55861 gggcgcaact ttcaccccgc ctcagcctgg ataccgcggc cttcatcaac gactacgaca
    55921 ggctgctcag cgtggacatg atcggcacgc catcgctcgt gatcccgcct ttcccgcctc
    55981 gcgccatgtc gtggatagca cgtgccggca acaatctgga aggccgcgcc acggggttcg
    56041 aaatctccct ggattaccgc ccctcgacca cctggcgcct gctgacggcc tactcgtatc
    56101 tcgatctgaa cctgcacacc aagcccggca atacggaact cctgccgctg cgtgccgagg
    56161 gtgaatcccc ccgccaccag atcagcatca tctcccgatc cgacctgcgc catgacctcg
    56221 aactcgatgt tcaggcacgt tacgtgagcg ctctgcccgc gcaggccgtc gccgactacc
    56281 tgactgcgga catgcgcctg gcgtggcggg ccagcaaatc ggccgatctg gcgctcgtgg
    56341 gccgcaacct gatgggccct gcccacacgg aattcggagg gctcgcactg atctccagct
    56401 cggcgtaccc cgttccccgg gagtattacc tcaccgccaa cctccgcttc tgacggcatc
    56461 ccgccttgaa ccgtccgcgt ccgcgccaag tcctcgcaga aatcatgcac gcgctgtccc
    56521 gctttccggg gcggtggcga ctgctgctga ccttgggcgt cgccacgctg acggcgccgg
    56581 cgagcagcgt ggagccgttt gcggaagccg aagtcaaggc cgccttcctc tacaacttcg
    56641 tccacttcgt ccgctggccg gacgtcgcac gcaagcctgg cgacccattc agattctgcg
    56701 tactggacga cacactcgcc ccgctcctga gcaaggccgt ccagggagaa aatgtagata
    56761 accatagcct tacggtcacc cgacagatcg aactgcgaaa cctggccgaa tgccagatcc
    56821 tctacgtcgg cgacgccgtc tggccggttc atcatgaact gcttcgccgc gcaaccgcgc
    56881 tcaggatcct cacagtcagc gacataccga gcttcacgga aaagggcgga atgattgcct
    56941 tgatccgcaa gcgcggaaga attcatccgg tcatcaacat ggacgccatc gcccgcgcag
    57001 accttcgcgt cagtgccaag ctgctgaatc tcgcgaccgt cgtgggcgac gacaagggag
    57061 gcgcagaatg atgcgtgaag cccttctgcg acagccgatc aaattcaaga tcatcgccat
    57121 cgcgctgatc accacggcaa tcggactgct cgtcgcactg acagcccagg tgagcagcga
    57181 attctcggcc accatcgaaa ccaaacagga acacgcggta tcactgacga ccatcctggg
    57241 attgaacagc accgccgccc tgtccttccg cgaccccgaa gcaggtcggg aaattctgtc
    57301 ggcgctggcc agcaaccacg aaatcatcgc agcgcgcctt cggcttccag acggcacgat
    57361 cttcgcgcgc ttcgacagca cacgccccga acacgctgac ctcatccagt ccattccgac
    57421 tgcaggacaa gccgaactgg cgcaccgaga gcacacactg accgcgttat ggcataaccc
    57481 tacacttgag gttgatcaag ctgttcgcct caacgacaag gtgatcgggc gcatagaagt
    57541 cttcttcgat ctgagcgccc tgatgaaact cgtccgcgaa cgcatcctgg ccgccgccgg
    57601 gatattactg gcggccaccc tcatcgcatg gctgctcgca tcgcgtctgc aacgggtcat
    57661 cgcagcaccg gtcgaggcac tggcagcaca aatgcaaacg gtgaccgaaa ctcgggatta
    57721 cagcgtccga gtggcgccgg gcggaaccga tgagatcgga accctcatga acggcttcaa
    57781 tgcgatgctg gaacagatcc agacccgtga cctccagctc agcatcgcca aagatgcggc
    57841 agagcaggcc aacaaagcaa agtccctgtt cctggcaacc atgagccatg agatcagaac
    57901 accaatgaac ggcgtgatgg gtatggcaga actcctagcg gactccaagc tcgacgcaca
    57961 tcagcaacgg ctccttggtc acattcagaa ctctgccgac agcctcctta gagtcatcaa
    58021 cgacattctg gacttctcaa aactcgaggc gggacgcatg gagctcgaaa atatcccgtt
    58081 cagtccaaag tccttgatag aggaaatcac cgcattcttt gaaccgaaag cccaggaaaa
    58141 gggaatcacc ctcaagccaa tcttccgaaa cgaaatacca cccctcgtac tcgccgatcc
    58201 aaaccgactc cgccagatcc tgatgaacct ggtcggtaac gccatgaaat tcacccaaac
    58261 aggcgagatc gttgtcgctc tcgaagcaca cccgatgcgc catgacgcgc ccgaacaccg
    58321 acaattacat ttttccgtaa aagacaccgg gattggcatc ccggagaatg tgatgcctca
    58381 tctgttctcc cccttcacac aggcagacag ctcacacgct cgacgttacg gcggaacggg
    58441 tttaggactg gctgtatgcc gcaaacttgt caaactcgcc gggggtgaga tcggcgtaga
    58501 aagcagcatc cgtcacggtt ccaaattttg gttcacagtg ccagttaaaa gtactgattc
    58561 aatcactgta ggtttaagtc agttcgaaag acaacaaccg catctaactg caacatcgaa
    58621 tgcaacaaaa ggattacgcg tgctcattgc cgacgatcat cgtatcaatc agcttctggc
    58681 gacaatgcag ctcgaagagc tcggatgtca cgtcgacaca gccggcgacg ggaaagacgt
    58741 tctgaacctg atcgccagga aggatttcga catcatactc atggactgcc aaatgcctga
    58801 attagatgga tacgaaacga cccgaatcat tcgcgcgcga caacggaaca atctccgcac
    58861 ccccatcatc gcggtgaccg ccaatgcgct cgacgaagac cgcgccacat gtctggaagc
    58921 aggcatggat gatttcctgc ccaaacccta tacccaaaac cagctcaagg aaatactttt
    58981 gaaatgggtc cctgacaacc aataaaaaag gccgcaccga tggcagcctt acaaaacaaa
    59041 gaacctaccc acacacctac actactaatg caagagatga tcgccgatca cccagtcacg
    59101 acctgcggaa acctaaacga agctgaaagg catcaccggg aaacaaaaaa acgaaaaaca
    59161 aacccgggaa agccaaacac tagaaacaca cagagcgagg cagcgtagaa ttcccacccc
    59221 tgtgaataag cgacaccgtg ctccgcacgc tccatccaga ggcagaaata aagaaatacc
    59281 aaataggccg cccccagctc agaaacgtga agccacgctg acttcaacgt ccgacgggga
    59341 agaacaccga acggcctgct tgaaataaaa ctcagattgg caagcaccgc cgcagcaatg
    59401 attaccgcca tagacggcac tttaacctaa cccttaaagc aaacacatct ttcaaacgct
    59461 ttaggttaaa gggaacccag cagcgcgtac gcacaaaccg acatcaaacc ctgtggcaag
    59521 atcccgagaa ctgcgatagc aatgccattc agcgacaaca tcacacgcat ttccgcagga
    59581 gcatgaatca ctgacgcatc caaaggttcg tcgaagaaca tcaccttaac aacacgtaga
    59641 tagtaataag cgccgatcaa cgacataaca accgcaagaa tcgcaacccc gatgtagccg
    59701 gccgcgacga cagcctgaag caccgaaaac ttcgcaaaaa atccgataaa gaaagggatc
    59761 ccggccatcg aaaacataga tatcgccatc acaccagcga accacgaatt gcgcttattg
    59821 agccccttga aatcatcaag cgtctcagcc tcgaatccca cacgcgacat gagaatcaaa
    59881 accccaaacg acgcgaggct catcagcacg taagcaacaa cataaaacat agacgagctg
    59941 tatgcattca gagcaaaatg tcgatccccg cctacaacac ctgagaccag cccgagcagc
    60001 ataaagccca tatgggagat gccagaataa ccgagcatcc gcttcagatt cgtctgggca
    60061 atcgccgcaa ggttaccaag aacaatggat agaactgcca taaacatcag catagactgc
    60121 cattgatctg caagatcaaa caatccattc accagcaacc tcatggccat agcgaacgca
    60181 gcaagcttgg gcgccgaagc gatcaagagc gtcaccgacg taggcgcacc ctgatatacg
    60241 tcgggaatcc acatatgaaa gggaacaacg cccagcttga atgccagccc ggcgacgaga
    60301 aagacaaggc cgaacatgag aaccgtacga ttgcccgcct ggtgataaac cgcctgcgcg
    60361 acaccagaaa tctcaagcgt tcctgtcgca ccgtaaacca tcgaaatccc gtagagcagc
    60421 aaaccagacg ctagcgcccc aagaacaaag tacttcattg cagcttccgt acaacggacg
    60481 gaatccctct gcaaagcaac cattgcatac agcgcaagcg aaagcaattc aagaccaaga
    60541 taaagcgtca ggaagtgatt tgctgacacc atcaccatca tgccaagcgt tgcgaaaata
    60601 accagtagat aaaactcagg ggattcgatg ttgcgatcat tcatgtaacc ccttgagtaa
    60661 atcagaacga caagtaccgc aaaataaagc atcagcttta ggaagtcgcc catcaggtca
    60721 tccacgtaca tattgctgaa cgtgaacgaa acctgaccat ccatggtgaa caaggtgacg
    60781 agcgctgccc cgaccaaggt aaggactgac aaccagtacc caacgctgcg agcatcttta
    60841 gcgaatgtcg tcgccatgag cacaacgata gccatgcagg caacaaacag ctctgacgca
    60901 gccgggtaga aatcgggagt gacgaaattc attattggac cactctgcaa gaattcaatg
    60961 acaacccgtg attacaactt gccagccgca acatgtttca gcagatcgct gaccgatgca
    61021 tgcataacgt tcgtgacggg gaaggggtaa atccccattg caagaacaca cacggcaaga
    61081 acaagaagaa aaacgaactc tcgattattg atgtccttca gttcagcaac atgatcgtta
    61141 gcaatagcgc caaacaccac gcgcttgtac atccacagcg tgtacgccgc cccaagtatc
    61201 agcgtcgtcg ccgcagcaaa acctacccaa aagttgtgct gaaccgcacc caatgcaacc
    61261 ataaattccc ctacaaaccc agaggttgct ggaagtccgg aattcgccat cgcaaacaac
    61321 atgaacagcg ctgcaaactt gggcatcacg ttcacaacgc caccataatc agcaatctgg
    61381 cgcgaatgca ttcggtcgta cagcacgcca atacacgcaa acatcgcacc ggaaacaaat
    61441 ccatgcgaga tcatctggac gagcgcacct tcgacgccga gcgggttaaa aataaagaat
    61501 ccgagcgtta caaatcccat atgcgagatt gacgaatatg cgaccaattt cttcatgtcg
    61561 gtctgaacga gcgccacgaa tccgatatag atgacggcaa tcagggaaag cgtaatcatc
    61621 atcggagcaa aagcttgtgc cgcgtccggc gtgataggca gcgaaaatcg aagaaaaccg
    61681 tacgccccaa gcttgagacc aatcgctgcc agaacgatgg aaccccctgt aggcgcctca
    61741 acgtgagcat caggcagcca ggtatggacc ggaaacatag ggaccttaac tgcgaacgac
    61801 accagaaacg cccaaaaaat caaggtctgg acatccatcc cgagcttaag ctcgtgccat
    61861 tcgagaatat tgaagctacc acccgactcc atgaagaggt aaagcagtgc gaccaacatt
    61921 aaaagcgaac ccgctagcgt gtagagaaaa aacttaatgg ccgcgtaaac tcgattaggc
    61981 ccaccccata caccgatgat gataaacaac ggaatcagag aggcctcaaa gaacacgtaa
    62041 aacagaacac catccagcgc agtaaatatg ccgtttataa gacccgacat aatcaggaat
    62101 ccggccatgt actgggaaac cttttcctga atcacatccc agcctgcaat cactacgata
    62161 acagtgataa aactattcag aaccacgaac aacacggaga tgccatcaac gccaagatag
    62221 taattgatgt tgaaacgcgg aatccaagca taagcttcaa caaactgcat agaacttgta
    62281 gcgacgtcga aaccagtgaa gagcggaata ctgaccgcga gccccacaag agcagtaaac
    62341 agcgcaacaa acctcgctac cggtgcattc ttgtcggaac ctgtcgccag ggccaccagg
    62401 ccgccgacga tcggcaccca aatcgcgagg ctgaggaatg gaatacccgt catcttaaat
    62461 ttccgtcatc tacaccttac ggtgcatcag ttcatcaaat ggtctgctgc gtcctgtata
    62521 ttccagtccg accgtcaacc ccgattgatc caaaaaacga ggagtacaaa aacgccgatg
    62581 atcatggaaa acgcatactg gtagatatga ccgctctgga agagcctggt gagctgagca
    62641 atccatccga caaatttggc tgaaccgttc accaccattc catcgatgat cgcctgatca
    62701 ccagctttcc acataccctt gcccaacaaa cgtgccccgc ccgcgaaaac ggtttcatta
    62761 aacttatcaa agtaatactt attttcaagc aatgtgtgaa tgggcttaaa ggccagcata
    62821 accttttcgg gaatccacgg ctggacaaga taaaggaacc aagctaacac gacaccgctc
    62881 atggcaagga agaacggcaa agtcgtgaat ccgtgcaatg ccattgcaac ggccccgtgg
    62941 aactcctcct ccagttcagc cataaccggg tgcgcatcgg aaaagtaaat tacacccttg
    63001 aagtattctc caaacaacat cggttcgatt gtgtaaaacc caaccaacat cgaagggatg
    63061 gcgagcatga cgagagggac tgttacgacc caaggagact cgtggggctt ttgcccaggt
    63121 gcgaggccgt gatggtgatc ttcgtcatgc gatgcatcac catcatcgtg gtgatgaaca
    63181 tcgtgatggc tctcatgcac caatccaaac cgctctttgc cgtggaagac aaggaaatac
    63241 atgcgaaagg agtaaaaggc agtaacgaat accccaacga tcacacatat gtatgcatag
    63301 cctgaccctg ccaaatgcga cacgccaacc gcctcaatga tcgagtcctt agaatagaaa
    63361 ccagagagaa acggaaaccc gattaaggcc aatgagccaa caagagatgt gatccaggta
    63421 acaggcatat acttccagag gccgcccatg ttgcgcatat cctgatcatg atgcattccg
    63481 ataataaccg aaccagctcc aaggaacaga agcgccttga agaacgcatg ggtcatcaga
    63541 tggaataccg cagcagagta tgcagaaaca cccagcgcaa ctgtcatata acccagctga
    63601 gaaagtgtcg aatacgcaac aacacgcttg atatcatttt gaacaatgcc aagaaagccc
    63661 atgaacaatg ccgtcgttgc accgatgatc agaacgaagc tgagcgcggt atccgacagt
    63721 tcaaacaacg gggacatgcg cgcgaccatg aaaatacctg cggtcaccat cgtcgcagca
    63781 tgaatcaacg ctgaaatcgg cgtaggacct tccatggaat ccggcagcca gacgtgtaag
    63841 ggaacctgcg cagatttgcc cattgcaccg atgaagagac cgatacagac tgcggtgatc
    63901 aatggccaac ccgttgccgg aacagtcatt ccagcaagat ctcccgcctt gttgaacact
    63961 tctgcgtagt tgaggctccc cgcgtaggct gcaatcaatc cgatgccaag tagaaaacca
    64021 aaatcgccaa cacgattaac aagaaaagcc ttcatgtttg cgaagatcgc ggtgggacgg
    64081 gtataccaga atccgatcag aaggtaggaa accagaccaa cagcctccca gccaaagaaa
    64141 agctgtagga aattgttgga catgaccagc atcagcatcg aaaacgtgaa aagcgagata
    64201 tagctgaaga aacgctggta gccaggatca tcagtcatat agcctatcgt atacacgtgc
    64261 accatcaagg acacgaaggt gacaacgatc atcatcatga ccgtcagagt atcgattagg
    64321 aatccgactt caaacgtcaa agcaccactt gttgcccatg tgtataacgt tccgttgaag
    64381 gtattgcctg cagccacgtc attgaaaatg aaaaccgagc agacaaacga gatagccaca
    64441 ccaaggatag tgactgtatg tgcccctgcc cgcccgatgg cctttccaaa aagaccagca
    64501 atgactgcac ccaaaagcgg agcgagcgga actacgaggt agagcttttg catatccgac
    64561 atcaaaaagc ttccttatcc cttgagagta tccagatcat caacatgaat cgtgcgcaga
    64621 tttcggaaca gagccaccaa gatggcaaga cctatccctg actcagcggc agcgacagtc
    64681 agaacaaaaa acacgaagat ttgccccgct gcatctccca gataacgaga aaacgctacg
    64741 aagttaatat ttaccgccag aagcattagt tcaatcgcca tcaaaagaac gatcagattc
    64801 tttctgttca agaatatgcc aactacgctg atcgcaaaga gaatggcacc caaaatcaga
    64861 taatgagaaa gtggcagcat cagcaatcca gaggtcttaa taacgcatta attattctga
    64921 atcagaaggc atcgaaacaa tccgtaggcg atcatttcgc tgaactgcca cctgagcaga
    64981 cgggtcaact ttcttaacac tatctcgctt acgcaaggtc agcgccacag cagcgatcat
    65041 tgcaacaaga agcacaatcg aggcaagctc aaacggataa gcgtactccg tgaatagaag
    65101 cctacccaac gcctttgtat tgctgtatcc ctgctcaggc gcaggaggca aaggtacgga
    65161 gtcaagtccg aaataagtgc ccagaaccat tcccatttcg acgaccattg caatcgcaat
    65221 caacgcacca accgggagat agctccagaa tccctgccga agcctttcga tgtttatgtc
    65281 gagcatcatc acaacgaaca ggaacagcac catcactgca ccgacataaa ccatcaccag
    65341 cgctatcgcc aaaaactcag cctgcagcag caaccaaatc ccgcttgctg aaacaaagga
    65401 gagcaccaag tacaaagcgg catgtaccgg attccgggac gtaattacct tcagcgaagc
    65461 tatcaccatg atcgccgaga agaaatagaa aactaccacc tgaaaatcca tgtctatatc
    65521 ctggttgcca attcactaca atcagcggta cttggattca acttcacgat cggcagcaat
    65581 ctgcttctcg tgtttttcgc ccacagcaag aagcatctgt ttcgtgtaat aaagatcgcc
    65641 ccgcttttcg ccatgatatt caaatattcg ggtttcaacc accgcatcaa cggggcacgc
    65701 ctcttcacaa aacccacaaa atatgcactt cgtcaaatcg atgtcgtatc gacttgtgcg
    65761 acgggatcca tcaccccgct gttcagattc gatggtgatc gccatagcag gacaaacagc
    65821 ctcacaaagc ttacatgcaa tacagcgttc ttcgccattc ggataccggc gcaatgcatg
    65881 taggccacga aaacgagggc tctgtggtgt cttttcttct ggaaactgaa cggtaatctt
    65941 acgtgcaaag agatgccgcc ctgtaagagc cattcctttc agcagttcct ttaaaaaaag
    66001 actgcctaca aaatccttca tagaacccat aatttacccc tcacttccaa accgacaggg
    66061 gagacatgat ccaaacagca acaagcatca cccagaaaag tgttaccgga ataaatacct
    66121 tccaaccaag acgcatgata tggtcatatc tgaaccgcgg aaatgtcgcg cggaaccaaa
    66181 ggaatagaaa caggatgaag gatgttttaa gtgccaacca aacaaagctg tctgggagaa
    66241 acccgaacgg agacaaccat cctccaagaa acaggataga cgtgagcgcg gagataagaa
    66301 tcatgttcgc gtactcacca aggaagaaca gtgcgaacgc cattcccgaa tactcaatca
    66361 tgtgtcccgc aacaatctcg gactctcctt cgacaacatc gaacggcgca cgatttgttt
    66421 ccgcaacccc ggaaatcaga taaaccaaga acattggaaa aagcggcagc cagttccagc
    66481 taaggaagcc aaaaccccaa tcggcccact gacccttgcc ttgactactg acgatatctg
    66541 tcaaattcaa gctcgatgaa atcattagga cactcaccag agcgaaaccc atagagattt
    66601 catacgaaac catctgcgcc gccgcccgca tccccccaag gaaagcatac ttggagtttg
    66661 atgcccatgc cgcgatgatt actccgtaga cttcaattga tgtaatagct agaaggaaca
    66721 gcaaacccgc atcaacatta ccaactaccc acccatctgc aaatggcact acggcccacg
    66781 cagcaagcgc cggccccaac gctagcactg gcccaacaat aaacaaactc ttgctggcgc
    66841 aactaggaac gattatttcc ttaagcaaga gctttacacc gtccgcgatc ggttgaagca
    66901 atcctttggg tccaacccga ttgggcccaa ttcgtacctg catgtaaccg atcaccttgc
    66961 gttcagccag agtgagatat gccacacaca aaaacaaggg agcaatgatc ccgacaattt
    67021 tcgctaggct ccagaccaaa ggccaaacat ccccgaaaaa ttcagcaacg ggctgcagga
    67081 cattttccat caggcacgct ccaccaaaat ttcagcgcta agactgccaa gtagtttggt
    67141 cgcatcatga gcaccggcaa gtcgtacgca accatctgca acaccgctat caacctgggc
    67201 aaaaatcgtc acttcgccag acttcgactt aacgcgtact ttctccccgt cacccacgcc
    67261 aatttccgca agtgtcttgc tggacattct tgcagtagga ataactgcat ccaatgtatt
    67321 ttgaagaggt tcggaccgac gaacgatcgg atcagcagca taaggcggaa tgtctgaaac
    67381 tcgctgcaat gagcaagcct tacgcttagc cgtcacaacg catcctttca gaacattact
    67441 caacttaaca tttgcatgat tagggcccaa actagcgtca cgcacatctt cgatacgttc
    67501 aaactcaaat ccaggaatac ctgccattga gcccagaact cgaagaattt tccatgccgg
    67561 acgagaatca cccgtactct taaccaccga ataaaatgat tgaattgcgc cttcggcatt
    67621 cacgtatgta cctgcggtct ccgtaaatgg agcgacaggc aacatgacgt cagcgtagtt
    67681 cagggcatct ttggatttaa acgcatttag gacgatgaca gtttctgcgt tctgcaaagc
    67741 agtccgcgca gcaactccgt cagccatgtc atattcgggc tcaacaccca gtaacacaca
    67801 tgctttaaga tctccattga tcatctgctg gacgttcatc cctgccaaat cggcatgagg
    67861 aatcgcccca gcaatgtacc cgcctacgct atttgccgct tcgccaataa cgccgaagct
    67921 cgcgcgtgag agacgtgcaa tctcttgaat caggatctga agttgaacag attccacgtc
    67981 ctgcgcggcc tgattgccga gccagattgc gatcttgcgt ccactggcaa tagaatctgc
    68041 aattgcgctc gattcattat cagcgccaga ctcgcttaca aaactggcca gctcgtcgct
    68101 gatggactga ctggtttgag aagcaattcg accggcaata cgcgccagta caaccttgaa
    68161 ttcatctgga gctactttgc agacatttgc gagcggcaat ctccagtcat caaatgccgc
    68221 tcccaatgcg ttgatcttaa gccccttctt cgctgcttga cggacccgct gcgcaagcag
    68281 cggatgatcc ttacgaaaga aagagccaac aatcaacaag cggtccagtt tcgatacatc
    68341 tgcgatcgac atgccgagcc atggcgcccc ttcaagctta tcatcagccg agaaatcaga
    68401 ctgcctaagc cggaagtcca cgttcccgga ctccagtccc cgcatcaagc gctgagcaag
    68461 atacatttcc tcaagcgtac tgtgcgggga aatccaggcc cctattgact taggcccgtg
    68521 ctccttggaa acggacttca gcccgttgac gacgtactcg agcgccactt cccaagtcgc
    68581 ttcctgccac accccacctt gcttgatcat cggtttgaga agccgctcag gtccattcac
    68641 tgcctcgtaa gagaaacgat ccctgtcgga aagccagcat tcgttaattg cctcgttttc
    68701 aagcggcaaa acgcgtttga ctttatcgtt cttaatctga acgatcagat tcgaccccaa
    68761 agaatcgtgc gggctcactg acttcctgcg cgacatctcc caagcgcgtg cgccaaaacg
    68821 gaacggcttc gacgtaagtg ccccaaccgg acacaggtcg ataatgttac ctgacaactc
    68881 agattcaagc gccttttcca caaaaggcat gatctcggcg tgctcacccc ggaatgcctg
    68941 cccgagctcc atcaaccccg cgatttccgt cgtaaatcga acgcagcgcg tgcagttgat
    69001 gcacctcgtc atatcggttg atacgaggga accgagatcc ttattcgaga cgactcgctt
    69061 ttcttcctgg tagcgcgagg aaacgccacc atatccgaca gatagatcct gcaactgaca
    69121 ttcgccacct tggtcgcaga ttgggcaatc aagcgggtga ttgataagca ggaactccat
    69181 cacacctttc tgagccttaa tcgcaggctc agaatgagtc cataccttca tgccgtttgt
    69241 taccggagtt gcacaagcag gcaatggctt gggagccttt tcaacctgaa caaggcacat
    69301 ccggcagttc gcagcaatgg acagcttctt gtgatagcag aaatggggaa cgtacgcacc
    69361 cactttactt gcagcctcta tgacggtact tccgtcagga acctgcaatt gcttgccgtc
    69421 aatttcgatt tctagcatgt cgagcccacg aaaatcttgc tgccttcgcg ctgaacatca
    69481 accggcacga gacatttttt gtgttcaatg tggtaggcga attcgtcgcc gaagtgcttg
    69541 ataaagcttt gaactggcat tgacgcagca tctcccaagg cgcagatcgt tctgcccatg
    69601 atgtttgctg taacactatt caacaaatcg agatcatcgg gacgtcccaa gccgttctca
    69661 attcgatgga caaccctata taaccagcct gtcccttccc ggcatggcgt acattggcca
    69721 cacgactctt caaagtaaaa gtaagagagc cgttcaaggg acttcaccat acaggtggac
    69781 tcgtccatga cgatcacagc gccagaaccc aacatcgatc ctgccttgga aatcgagtca
    69841 tagtccatcg tacaatccat cataacgtgg cccggcagca ccggagcaga cgacccacct
    69901 ggaattacac ccttcagttt acgcccacca cgcatcccgc ctgccatttc gagcagcacg
    69961 ggaaaaggtg taccaagcgg gatctcgtaa tttcctggcc gatttacatg accagaaact
    70021 gaaaagagct ttgtgccgcc gttattaggc tttcccagcg caaggaacgc gtccccgccc
    70081 tcgcgaatga tgtatggaat tgaagcaaat gtctcagtgt tgttgatcgt cgttggcttc
    70141 ccgtacagac caaaactagc cggaaaaggc ggcttgaaac gaggctgccc tttctttcct
    70201 tcgatcgact ctagcaatgc cgtctcttca ccacaaatat aggccccata gccatgatga
    70261 gcgaagagct caaaactaaa atccgagcca agaatgtttt ccccaagaag cccagcgtcc
    70321 cgcgcctctt gcagagcctc ttcgaaacgc tcgtaggtct cgaaaatttc tccgtgaata
    70381 tagttgtaac cccgctcgca ccccatcgca tatccggcga tcaccatacc ctcaattact
    70441 gaatggggat taaaccgaag gatgtcacga tccttgaacg tgccgggctc accctcatcg
    70501 gagttacagg cgatatactt cgcgccaggg aacgcccttg gcatgaaact ccatttcaaa
    70561 cccgttggaa acccggcacc accccgaccg cgcagcgcag attttttgac ctcagcaatg
    70621 atttgatcag ccggaatttt atcggcaata atctttttaa gcgcctcata tccaccgcgc
    70681 tttagatagt cggtgaggcg ccaagtcctg tcgccgtcaa gcccagagag aataatgcct
    70741 tgagcgctca ttttttaaga tcagccagaa gttgatcgat tttctcttcc gtcatccaac
    70801 tgcacatgcg atggttgttt aagagaagta ctggtgcgtc accacatgca cccatgcatt
    70861 ccccttcttt cagcgtaaaa agcccatcag cagtcgtttc gttaaaatca atcccgagtt
    70921 tcttcttcag gtactcagct gcatgtacgc cgccggagag aacgcacggc aagttggtgc
    70981 aaatggtaat tttttttgcg ccaactggcg tcagatcgta catgttgtaa aacgtcgcaa
    71041 cctcgtaagc cgcaattgaa ggcatatcga gataccgagc aacgaactcg atcgtctctt
    71101 tggctagcca gcctttttcc atctgcgcaa ttcgcaatgc agacatgcaa gccgactgct
    71161 tctgatcaac aggatattta gctatctcgc gatcaatttt tttcaaagat tcaggactaa
    71221 gcatattccg ttcgtctgcc tgcaagaata ctgtttacac cacttggatc aacatatggc
    71281 ttacatttac cggtctacgt cgccaaacac gatgtccatc gtgccaatga ttgcaacgac
    71341 gtctgcaatc atgtgtcctc gtgacatcgc atccatcgcc gacaaatgtg caaatccagg
    71401 tgagcgtaac ttcaacctgt acggtttatt tgcaccgtca gagatggcat ataccccaaa
    71461 ctctcccttg gggtgttcaa cgggcgcata cacctcaccc gctggaacat gaataccttc
    71521 cgtgaagagc ttgaaatggt gaatcagctc ttccatgttt gccttcatct gctctcgtgc
    71581 aggaggggct accttgtgat tatcggatat taccgggccg gggtttttgc gaagccattc
    71641 gatgcattgc ttgacaatcc gattggattg acgcatttct tcgatgcgac acaagtaacg
    71701 gtcgtagcag tcaccagccg ttcctatcgg aatgtcgaag tccatttggc tgtaaacttc
    71761 gtacggctgc ttttttctta aatcccattc gatgccggaa ccacgcaaca tcacaccggt
    71821 gaatccaagc gccaaggcct gctccggcga aacaacaccg atcccaaccg tccgctgctt
    71881 ccagattcgg ttatccgtca gcagagtctc gtattcatca acataacggg ggaatcgttc
    71941 acagaaatct tcaagaaaat cgagcagtga gccctgacga ttctcattga gctcacgaac
    72001 gatcttttcg cttttccact tggatacttc atactgcggc atgcgatcag gcaaatctcg
    72061 ataaacacca cctgggcggt agtatgccgc atgcattcga gcacccgata cggcttcata
    72121 tgcgtccatg agatcttcac gctctctgaa cgtgtaaaga accatggtca tcgccccgat
    72181 gtcgagtgcg tgagttccga ctgccaaaag gtgattcaaa atccgcgtga tttcatcgta
    72241 catcacacga atgtattgtg cccggagcgg cacttccaat ccgagaagac gctcaatagc
    72301 catgcaataa gcgtgctcgt tgcacatcat ggagacgtag tcaagccgat ccatgtacgg
    72361 aacagattga atccatgtcc tggtttcggc aagcttctcc gttccccgat gaagaagtcc
    72421 gatatgagga tccgcacgct cgacaacctc gccgtcaagc tccaatacca atcggagcac
    72481 gccgtgcgcc gacgggtgct gtggtccaaa gttgatcgtg tagttacgaa tttcagccat
    72541 gaccaacatc cccgtaggaa tcttcgcgca cgattctagg tgtattttct cgcggttcta
    72601 tggagacagg ctggtagatc acacgcccca aatcctggtc gtagcgcatt tccacaaagc
    72661 ccgacaccgg aaaatctttg cggaacggat gaccaacaaa cccatagtca gtcaggatcc
    72721 ggcgcagatc cggatgcccc atgaacatca cgccaaagag atcaaaggcc tcgcgctcga
    72781 accaattcgc gctgggccag accgcacaaa gggaagcgac catggggaaa tccgaatctt
    72841 cggcaaaagt ccgaaggcgg agacgtacat tgtggcgaat cgaaagaagg tgcgaaacca
    72901 ccgcaaagcg tcccgaaaaa gaatcagcca atgcatgcac tgaataatct acacccgtta
    72961 aatctaccag ctgttcaaat gcgagttctg gatcatcccg caaggactcc gccacagcca
    73021 aataattttc agagcgtact tcgagcgtca cttcgccggc agcgtctatg agacgcacga
    73081 tgtcgtctgc aaatttttcg ctgaggcatc gggtcaaatt ttcgagctta gaactcatga
    73141 tcgcctgctg ttaccgagca atagtacatt cgcgcttgat cttgttctgc aactgcacga
    73201 ttccgtacaa cagcgcttcc gccgtaggag gacagccggg aacgtagacg tccactggaa
    73261 caatccgatc acagccacga acaacggaat acgagtagtg atagtagcca ccaccatttg
    73321 cacaagatcc catcgaaata acccaacgcg gctctgacat ctggtcataa accttgcgca
    73381 aagccggcgc catcttgttg cagagcgtac cggccacgat catcaggtcg gactgccggg
    73441 ggctaggacg aaagacaaca ccaaatcgat caaggtcgta ccgggaacag ccagcatgga
    73501 tcatttcaac cgcacaacag gccagaccaa aggtcatcgg ccaaagggaa cccgtacgcg
    73561 tccagttgat cacggtatca agggaagtgg ttacaaaccc ttccttgaaa acgccttcga
    73621 tgctcataaa acccctttaa gaacgagtgc gctatcggga cggacgatca gcctctattc
    73681 cgccaaaaga tcactcccaa tcgagcgcac cttttttcca gacgtagatg tagccaacca
    73741 gaagaatgcc cagaaagacc agcatctcga agaaaccgaa cgaccggacc gcctcagtac
    73801 cagcaatctc ccggagaatg gtcgcccatg gaaaaaggaa ggccacttcc agatcgaaga
    73861 gaataaagag gattgcaagc agatagtagc gcacatcaaa cttgatacgc gcatcttcga
    73921 agggctcgaa gccgcactcg aacggggaga gtttctcggc atcaggcttg ctgggcccca
    73981 aaatccagcc tgccaagatc gggatcaatc cgaacccagc cgcaaacagg ataaacacga
    74041 gaactggaaa atacgcttcc aacatcactt gaccccccgc aactgatgac cagcaaattt
    74101 actgctattt gcaagcaaac gcccgcatgt gcgggcggct tgctgtctta ttcgtgctgg
    74161 tgccgacgat gagactcgaa ctcatacggc tctcgccact accccctcaa gatagcgtgt
    74221 ctaccaattt caccacgtcg gcacgtctgt ttgactcagc ggattataag acaacccgac
    74281 cgcagcgcca agcattactt aaaactatcg aggaatgtct tttgccttgg atgcgtcttc
    74341 gatcggcgga gggggcggtg tggcgacacc gcctgataca ggcgggtgga ccaccccctc
    74401 catgacgctt gtgggctgct tccgctcggc accacccgca agatagctca gacccagact
    74461 cgtcacgaag aatacagcag caagcacacc tgtcgtccga ctcagaaagt tagccgaacc
    74521 tgacgaaccg aagaggctac ccgaagcacc actgccaaat gccgcgccca tgtcggctcc
    74581 tttgccgtgc tgcaccaaca ccagtccgat gacaccgagc cctgcaagca cgtgcaccac
    74641 aagtaccaac gaaaacatca tgttacccac aaatctctcc cgatcctgta ccgaccgacg
    74701 cggcctgaca cacggcaaaa aagctatcga acttcaagga tgccccaccg acaagcccac
    74761 catctacatc atcctgcgag aacagcagtg cggcgttttc agccgtcaca ctccccccgt
    74821 acagaatacg aagcttgtcg accacatgct ctccatgcat agatcccagc cattgacgga
    74881 tgagacgatg cattgaccgc acctcttccg gtgaggccgt tacacccgta ccaatggccc
    74941 agataggctc gtatgccacg accacgcgct ccaactcgtc agcactcagc ccggctaaca
    75001 cacttagctg ccgtttcacc accgattcag caacacccga acgacgctca gccagcgctt
    75061 ctcctacgca cagaatcggg cttaacccaa cccgaagggc tgcgcgcact tttcggccta
    75121 caacgtcatc cccctctccg aacagagcgc gtcgctccga gtgcccaaca atcacccagc
    75181 tacaggaaaa atccctcaac atcccaccac tcacttcacc ggtgaatgca ccagaatcat
    75241 gcatgttgag atcctgcgcc ccccaggcga ccgaggactg gctcagcatc tgctgtgcct
    75301 gggaaaggta agggaatggc acacaaaccg ccacgttcac acccgcgagg gcttcagcct
    75361 gacgcaatga tgaaagtagg gacgcattct gccccagccc tccgttcatt ttccagttgc
    75421 caaccaccag ttttttccgc acgacttcca ctcggacaaa tttttcgatt ctagtgcaac
    75481 aagcgcccag ccgtcaaagc atcacttaat ccgtgtttac tgtgcgcctg ggacgttacg
    75541 ccccttggtg cgtgtcgtca catggcagcg gcacgcacaa cttccgcaat cccatgggcg
    75601 agctgttcaa cgatccgggc atcctgcccc tcgaccatga cacggaggag cggttcggtc
    75661 ccggacggac gcagcaacac gcgtccggac tgaccgagtg tcgccttcgc gtcatcgact
    75721 gctcgagcaa tctcgcggct gtctgtccaa acgaacccgg aacgcaatgg cacattgacg
    75781 agcttctgtg gataaaactc gaggtcctcg cacatctgct ccagcgtgag tccctgctta
    75841 cgcaatgcgg ccagcacctg aagccccgcg acaatcccat ctccggtcgt gtgcttatcg
    75901 aggcagatga tgtgccctga attctccccg cccaggcgcc accgacgctc gtgcatctgc
    75961 tccaggacat agcgatcacc cacctttgca cgcgcgaacg gcagccctag acgggcaata
    76021 gcatgctcga atcccagatt gctcatcagc gtcccgacga ccccggctcc gggagaccct
    76081 gcgaccagcg ccgctttggc gatcacatac agcaacttgt caccgtcgta cagattgccg
    76141 gctgaatcag ccatcatgat ccggtcgcca tcgccgtcaa gcgcgatgcc gatgtccgct
    76201 ccgctcgcca ggacggtatc gcgcaaatgc gttggtgatg tggcgccaac accctcattg
    76261 atattgaggc cgttgggctc gactcctgtt gcgataacct ccgcgccgag ctcatggaag
    76321 accttgggcg ccaccatgta ggcagcgcca tgggcacagt ccacagcgat cttcatgcct
    76381 ctcagatcga gttcattggg gaaggtgctc ttgcagaact caacgtaacg tccgcgcgcg
    76441 tcatcaatgc gacgtgcctt gccaagagcg gccgaagact cacagcccat gggttcgtcc
    76501 agacgcgcct cgatttcctg ctcaactgcg tctggcagct tcgttcccga cgcggagaag
    76561 aacttgatcc cgttgtcgtc gtacggattg tgggaagcgg agatcacgac ccccacctgg
    76621 agcctcaaag cgcgcgtgag gtaggcaatc gcaggcgtcg gcagggggcc agcgaggtaa
    76681 acgtccaccc cggcagccga aaaacccgct tcgagcgcag attcgagcat gtaccccgag
    76741 attcgggtgt ccttgccgat gatgaccgcg gggtgttccc caggtcgcag gttttcatgc
    76801 gcaaccaatg tcttcccagc ggcataaccc aaacgaaggc caaaatccgg cgtgatcgga
    76861 agctcgccca cccgaccgcg aacgccgtcg gttccgaagt atcttcttga catattccct
    76921 cctaatcgct gggttcgcag tggcgcctca tgcggaattc tgccgcttca agccccgaaa
    76981 aaacacgaat aacatcacgc gtcgctgcaa cgtcgtgtgt cctgacaatg cgggcgccac
    77041 gttgaatcgc ccacatcgca gcgaccgcac ctgcaacttg ccgctcacca actggtcgtc
    77101 cggtcaatgc tccgagcatc gacttgcgag acatgccgac caatacggga aatcccgact
    77161 tcccgaagac ctccagccga gcgaggagcg ccagattgtg ctccagcgtc ttaccgaaac
    77221 caaatcctgg atcgaccaaa atccgctctg cagcaacccc ggcctgaagc agcaccttca
    77281 cgcgctcatc cagaaagatg cgcacctcgt ctacgacgtc gatataaaca ggctcaatct
    77341 gcatcgtacg gggctctccc tgcatgtgca tgacacagag cgccgcagag gagtccttga
    77401 ccgcttccaa tgcaccaggc tgccggaatg cggcgatgtc attgatcaga tcggcgcccg
    77461 cgtcgagtgc cgctttcatc acacgcggct tgacggtgtc gaccgacagg ggaaccccca
    77521 acccctgcaa agccgcaatc actggaacga cacgctccag ttcttcagcc tccgaaacac
    77581 ctgcagcccc tggccgagac gactcaccgc cgacatcgat cagatccgcc ccttgctcca
    77641 ccgcacgttc ggccgcccgc agcacatggt caaaagcctt acagccgtcg ccggaaaagg
    77701 aatcttcggt gacgttgacg atggccatga tcttcggacg gccaagatcg agctggaaac
    77761 gtccacaacc cagaagcata ttagcagtct agacagaggg caaacgaaag aggccggctg
    77821 cgccggcctc aaaatgaaac ggacatgcct caggcagcag tggccggcgc cgtgggggct
    77881 gcacctggcg cgtcgtcact gggtcggcgt ggcacctgag aaggctttgg tgggcggggc
    77941 gtacggccgt ccatgatgtc attgatctga tccgagtcga tcgtttccat ctctagcaag
    78001 gcggtcgtca tggcttcgac cttctcacgg ttctcctcga tgagacgccg ggcgagcgcg
    78061 tactgttgat caaggatacg ccggatctcc gcgtcgacct tctggagcgt ggcttcggac
    78121 atgttcttat gggttgtcac cgaacgccca aggaagacct cgccttcctc ctcaccatat
    78181 accatcggcc ccagattgtc tgacatcccc cattgcgtca ccatgcgacg cgccagatcc
    78241 gtcgctcgct ggaaatcgtt ggaggcaccc gtggtcattt gattcatgaa gatctcttcg
    78301 gcgatccgcc cgccgaacaa tacggcaatg gtctggagca gacgctcacg gtcctggcta
    78361 taacgatcct gttcgggcag ttgcattgtc acgcccaagg cacggccccg cgggatgatc
    78421 gtaaccttat gcaccggatc agtcttggtt aaacacttcg ccacgacggc atggccggac
    78481 tcatggtaag ccgtgttgcg tcgctcctcc tcgggcatga ccatggatcg ccgttcggcc
    78541 cccatcatga tcttgtcctt ggcacgctca aaatcatcca tatccacaag gcgcttgctg
    78601 cctcgcgcgg caaacaatgc ggcctcattc accagatttg cgagatctgc acccgcaaat
    78661 ccaggacagc cacgtgcgag caccaaagcg tccacgtccg gcgcaatggg caccttgcgc
    78721 atatgaacac ggaggatctg ttcccgcccc cggatatccg gaagcggcac caccacctgg
    78781 cggtcaaagc gcccaggacg cagcaacgca ggatccagta catcaggacg gttggtcgca
    78841 gcgatcacga tgactccaga cacgccttcg aaaccgtcca tttccaccag cagctggttc
    78901 agcgtctgct cacgctcgtc attgccgccc cccagaccag cgccccgctg acggcccacc
    78961 gcatcgattt cgtcgatgaa gatgatgcac ggcgcctgct tcttggcctg ctcgaacatg
    79021 tcgcgcaccc gagcggcacc cacaccgacg aacatttcga cgaagtcgga gcccgagatc
    79081 gagaagaaag gtactttcgc ttcaccagcg atggccttgg caagcaaggt cttgccggta
    79141 ccgggggagc caaccatcag aacacccttg gggatatggc cacccagctt ctggaatttc
    79201 gacggatcac gcaggaagtc gacaagttct ccgacttctt ccttggcctc gtcacagccc
    79261 gccacatcag caaaggtcac cgtgttggcg gattcgtcca gcatcttggc cttcgacttg
    79321 ccgaaagaaa acgctccgcc ccgtcccccg ccctgcatct gtcgcatgaa gaacacccac
    79381 acgccaatga ggaggatcat cgggaaccac gaaacaaaga tgttcatcag gaaggactgc
    79441 tcttcctcag gcttggaggc agtgaccttg acaccgtagc gcatcaggtc acccaccatc
    79501 cagatgtcct ggacaccggg cgtgtaaacc gtgacgacac gcccgtcctg ggtcgtggca
    79561 cggaccgtcc gtccgtcgac gacagccttg ccgatcttac cctgcttggc gtcctccatg
    79621 aactgggagt attcgaccga attctgggca gtctgacgat tgttgaactg gttgaagacg
    79681 gtcatcagca caacgccgat caccaaccag atcgcgagat tcttgaagag gttgttcaac
    79741 tcgattcctt gatcgcccaa caggggcgga cgtacagtgg actgattcta tgtctgatcg
    79801 ttcgccggat caaatgatcg tcaaatttgc gggcatccgg cgcacgatcg ccggatcagg
    79861 accgtttgcc cgtccccagc agatagactt ccgcgcttcg acctcgagac gcctggggct
    79921 tgcggacgac gacactcctg aacgcttcac ccatcgcctt gcggtaatca tcgaaccctt
    79981 cgccctggaa caccttgaca agaaagcgcc cgttggcggc cagatgacgc gcggcgaaat
    80041 ctagtgccag ctccgccaga tgaatcgacc gagcctgatc actggacgga attcccgaga
    80101 tattcggcgc gatgtcggac agcacaaggt ccacctgccc atccccgagc tgcacttcga
    80161 acgcctcgac gaatgcgtcg tcgtgaaaat ccccctgcat gaaatgcacg cccggaatcg
    80221 gctccatcgg cagcagatcc agtgcaaaga cccgtccggc gccaccgacc ttgccgacag
    80281 ccacctgact ccatgacccg ggcgcagcac caaggtccac gacggtcatg ccgctccgga
    80341 acagtttgtc cttttcgtcg atttccatca gcttataggc agctcgagcg cgcattccct
    80401 ccgtttttgc gcgctgaacg aaaggatcgt tcacgtgttc catcatccac gatttactgg
    80461 tcttgctacg cttcatggcg gtataattac gccctttttt caaagagttg gaatgatgat
    80521 tgaactcacg ccagcacagc ggcgggcctt tcgcgcgcag gctcaccacc tccatccggt
    80581 tgtcagcgtc gccagcaacg gccttacgcc ggcggtcctg aacgaggtcg atgccgccct
    80641 gcaggcccac gaactcatca agatccgcgt gtatggagag gaccgggccc agcgcgacgc
    80701 aattctcgcg accgtatgtg tggaactcgg tgcggccgcc gtacagcaca tcggcaagac
    80761 gctggtcgtc tggcgtgagc gccgcgaaga cgaaaagacg gtggcaccgg tcaccgataa
    80821 accgcgacgc tccctcgcca aggccaagtc tgccaaggtc ttgtcggcac gccgtggcgt
    80881 gtcgtccttg aagcctaaat ccgcccagaa gaagccttac tggttcgcgt ccccgtcgga
    80941 ccgcaagccc cgtcgggaag actgaatctg tcacacatgg ccgtgtcagc gcagaccgcg
    81001 gccctgtaca acaacgagca gcacgcccag aacactttcg accaggtaaa cgacgctcga
    81061 cacgccgtgc catgcgacga accggtcacg cacggcgcct tccatcactt cccgcggcag
    81121 gctttccgcc ttgagctgag ccaggacggg ctggataccg aacgcgccgg caaccgtcag
    81181 aagcagcatc accagcacca gccaaaacac gctgctcttg aacgcagccc cgttcttgcg
    81241 aagcgcgaca aagaccagca ggtaggccgc ggaggccatg cccaaccagc ccacgtacgt
    81301 aaatagctgc ccagccagac gacctgccaa ggcgcggtcg tccagcatgt ggaacaaggt
    81361 gggcgccacc atgtagccca ccgtccacag ggcgccaacc cacagggtga tgagcagact
    81421 gtacacgtgt tctgccaacc gggtcattgc tgcgcaccga gcatcatgac gctgccactc
    81481 aaacgtaacg gacgtcgatc aattccagct cgcgcacgcc acccggcgcc tgcacttcag
    81541 ccacatcgcc ggcaaatttg ccgatcagcg cacgggcgat gggcgaactg atggcgatct
    81601 tgccttcctt gatgtctgcc tcgtcgtccc cgacgatctg gtaactcacc ttggcgccgg
    81661 aatccagatc ctccagatcc accgttgcgc caaacacgca gcggccgtcg gcgtcgagca
    81721 gcgtgggatc gatgatctgg gcgttgccga gcttgccttc cacctcgcgg atacggcctt
    81781 cgatgaaacc ctgacgttcc ttggcggcat cgtattcggc gttttcggac agatccccgt
    81841 gggagcgcgc ctctgcaatc gcagcgatca cgttgggacg atccacggtc ttcagacggt
    81901 gcagttcctg acgcagcttt tcagcaccgg caacggtcag cggaatcttg ctcatgcctg
    81961 ggacagctcc gcgtgaaggg cctgcagcga gtaggcttcg aggccggaca gatgacgcat
    82021 gcccatgcac gccgcacggg ccccctcgat ggtggtatac acggtgacct tggccgccag
    82081 cgcgctagtg cggatggagc gcgaatcggt caccgcctgg cgcttttcct caacagtgtt
    82141 gatgaccagg gcgatctcgt tgttcttgat catgtccacc acgtggggac gcccctcatt
    82201 caccttgttg accgccgaca cgggcagacc ggcggcggcg atggcgccgg cggttccacg
    82261 ggtcgccacc accgagaagc ccaagtcgac cagttcgcgc gccacctcaa cggccttcgg
    82321 ccggtcggtg tgcttgacac tgatgaaggc cttgccggcc gtcggcagac gcacgccggc
    82381 gcccagctgg ctcttgacga aggcctccgc gaaggtccgg ccgacgccca tgacttcgcc
    82441 ggtggacttc atctcgggcc cgagaatggt atccacgccc gggaacttga cgaaggggaa
    82501 taccgcttcc ttcaccgcgt aatacggcgg caccacttca ccgctgacgc cctgatcatc
    82561 caggctgcga ccggccatgc agcgggcggc aatcttcgcc agcggcaggc tgcaggcctt
    82621 ggagacgaag ggcacggtac gggacgcacg gggattcacc tcaagcacgt acaccaccgc
    82681 atcgtcgcct tcaccctgga tggcaaactg cacgttcatc aggccgacca cgttgagggc
    82741 gcgggccatg gcttcggtct ggcggcgcag ctcatcctgc agcttggccg acagggtgta
    82801 gggcggcagg gaacaggcgg agtcaccaga atgcacgccg gcctgttcga tgtgttccat
    82861 gatgccgccg atgatgacgc ggtggccgtc ggacagcgca tccacatcca cctcggtggc
    82921 atcgttcagg aaacggtcga ggagcaccgg cgaatcgttg gagaccttca cggcctcgcg
    82981 catgtagcgc tccaggtcct tctgctcatg gacgatttcc atggcgcgac cgcccagcac
    83041 gtagctggga cgcaccacca gcgggtagcc gatctcggcg gccagccgca cggcgtcttc
    83101 gggcgtgcgc gcggtgcggt tgggcggctg cttgaggccc agttcgaaga gcagcttctg
    83161 gaagcgctcc cggtcctcgg cggcgtcgat catgtccggg ctggtgccga tgatgggcac
    83221 gccgttggcc tcgagttcgc gggcgcgctt gagcggcgtc tggccaccga actggacgat
    83281 cacgccaacc ggcttctcga cggcgacgat ctcgaggatg tcttccaggg tgagcggctc
    83341 gaaatacagc cggtcggagg tgtcgtaatc ggtggacacg gtctccgggt tgcagttgac
    83401 catgatggtc tcgaacccgt cctcgcgcag ggccagggcg gcatggacac agcagtagtc
    83461 gaactcgatg ccctgaccga tacggttcgg accgccgccg agcaccatga tcttcttgcg
    83521 gccggtgggc tgggcctcgc actcctcctc gtaagtggag tacaggtagg ccgtggaggt
    83581 ggcgaactcc gccgcacagg tgtcgacgcg cttgaacacc ggccgcacgc cggcggtatg
    83641 gcggtgcagg cggaccgagg tttcgtcggt ggcgagcagc ttggccaggc gccggtcgga
    83701 gaagcccttg cgcttgagct cgcgcagttc ggcggcggaa atgcccttca gggagcgtcc
    83761 ggcgagggtc ttctcggtct tgacgatgtc ctcgatctgc accaggaacc acggatcgat
    83821 gcgggtcagc gcatgcacct gatccaggct catgccttcg cggaaggcct ggcccacgta
    83881 ccagatgcgc tgggcacccg gatcgccgag ttcgcgctcc aggtcgtcct gatcggcttc
    83941 cacctcgtcc aggccgtagg cgccggtctc gaggccgcgc agggccttct ggaaggattc
    84001 ctggaaggtg cggcccatgg ccatcacctc gcccacggac ttcatctggg tggtgaggcg
    84061 gtcattcgcc agcgggaact tctcgaaggc gaagcgcggg atcttggtga ccacgtaatc
    84121 gatagacggc tcgaaggacg ccggggtcgc gccgccggtg atgtcgtttt tcagttcgtc
    84181 cagcgtgaag cccacggcca gcttggccgc caccttggcg atggggaagc cggtcgcctt
    84241 ggaggccagc gcggacgaac gggacacccg cgggttcatc tcgatgacga tcatgcgacc
    84301 gtccttggga ttgatcgcga actgcacgtt ggacccgccg gtatccacgc cgatttcccg
    84361 cagcacggcg atggaggcgt tgcgcaggat ctggtattcc ttgtccgtca gggtctgggc
    84421 cggcgctacg gtgatggagt cgccggtgtg cacgcccatc gggtcgaggt tctcgatgga
    84481 gcacacgatg atgcagttgt ccgccttgtc gcggaccact tccatctcgt attccttcca
    84541 gccgatcagg gattcctcga tgagcagctc gttggtgggc gaagcttcca ggccgcgctt
    84601 gcagatctcg acgaattcct cggtgttgta ggcaatgccg ccccccgtgc cgcccagggt
    84661 gaaggacgga cggatgatga ccgggaagcc gatctcggcc tgaacctggt gcgcctcttc
    84721 cagcgcgtgg gcgatgcctg aacgggccga gccgagcccg atgcgggtca tggcggcctt
    84781 gaacttctgc cggtcctcgg ccttgtcgat ggcctcttcc ttggcgccga tcagctcgac
    84841 accgtatttc tcgagcacgc catggcgggc cagatcgagg gcgcagttca gcgccgtctg
    84901 gccacccatg gtgggcagca cggcatcggg gcgctccttc tcgatgatgc gctcgaccac
    84961 ctgccaggtg atgggctcga tgtaggtgac atcggccgtc cccggatcgg tcatgatcgt
    85021 ggccggattg gagttcacca gcacgacctt gtaaccctct tcgcgcaggg ccttgcaggc
    85081 ctgggcgccg gaatagtcga attcacaggc ctggccgatg atgatcgggc cggcgccgat
    85141 gatgagaatg gtctggatgt ctgtacgttt gggcatgatt cttcagctgc gtgcgagcgg
    85201 tattccggga gcggcgaacc tggggcccgc cgggtcggtg cgtcaggacg cgcgccgtcc
    85261 ctccatcagg gcgatgaaac ggtcgaacag gtaggccacg tcgtgggggc cgggactggc
    85321 ttccgggtga ccctggaagc agaacgccgg cacgtcagtg cgggccaacc cctgcagact
    85381 gccgtcgaag agggacacat gggtggcgcg aaggttcgcc gggagcgttg caacatcgac
    85441 ggcaaagccg tggttctggc tggtgatgag cacctgaccg gaatccagat ccttcaccgg
    85501 gtgattggcc ccgtggtggc cgaacttcat cttgagggtg cgagcgccgg aagcgagggc
    85561 gagaagctgg tgcccgaggc agatgccgaa ggtgggaatg cccttggcca gcacttcgcg
    85621 gatggcggcg atggcatagt cgcagggctc cggatcgccg ggaccgttgg acaggaacac
    85681 accgtccgga ttgagggcca ggacctccgc cgcgggagtc tgggcaggaa ccaccgtcag
    85741 gcggcagcca cgctcggcca gcatgcgcag gatgttgcgc ttgacgccgt aatcaaaggc
    85801 caccacatgg aaacgggggg cttcctgcgg aacatagccc ttgtcgagct gccactcgcc
    85861 ttcggtccag gcgtaggatt cggcggccga caccaccttg gccaggtcca tgccggccag
    85921 accggggaag gcgcgggctt cggcgagggc cgcctcggag ctgagctccg ccgtggtcgc
    85981 cgcggtgacg atgcagccgc cctgggcccc tttctcacgc aggatgcggg tcagcttgcg
    86041 ggtatcgatg cccgcaatcg cgacgatgtt ctcggccttg agataggcat ccagagactg
    86101 ctcgttgcgc cagttggacg ccagcagcgg cagatcgcgg atcaccaggc cagcggcata
    86161 caccctgccc gcctcgcagt cttcctggtt gatgccggtg ttaccgatat gcgggtaggt
    86221 cagggtgacg atctggcgac agtagctggg atcggtgagg atttcctgat aaccggacat
    86281 ggaggtgttg aacaccacct cgccggtggt catcccgacc gctccaatac ctttaccgta
    86341 aaacaccgtc ccgtcggcaa gggcaagaat ggcgggcggg aaagcagaca cggcggactc
    86401 ctcgaaactt tttgatggaa atgctcacat gggtagccgg gctacacatg aaaaacggga
    86461 tcagcccgtg aggctatccc gctgataacc gcaagattat agcccgaggc accgctccgg
    86521 gcaaatgcgt cccacccgat gccccaatcc ggtgcagtgt ttcaccctcc ccgccgggcc
    86581 cgccgcagcg ccccggccag aacacccagc tcgcgcatca attcattcac acagcgccgg
    86641 cattccgcct cgtcggctgc acgcgccgcc ttttccacct gtgccgcgct ggctacgacc
    86701 cgcgacgcgc tgaacacacc ggcggaaccc ttgagcgtgt gcgccacatc ccgcaggcgg
    86761 gcaaagtccc gtgtcgccaa tgcggcatcg agcgctttgc ggttacggtc gaaatcgttg
    86821 aaaaagatgc ggatcaggtt ctgcaattga tcctcgtcgc catccagcaa atcccgggtc
    86881 gcgcccagat ccgcgatctc ctcgggctcg atggctgcgt cgccattgag gaggcccgcg
    86941 tccgccaggt gctcgtccga tgcggcaaag ctctgggcct gtcgaacccg cgcgatggct
    87001 gcgtaaagtt cgggcggacg gatcggcttg gtgacgaaat cgtccatgcc ggccatcagg
    87061 caggcttcct tgtcgccctg catcgcgtgg gccgtcatgg cgatgatcgg caccgacagc
    87121 gattgacggc cggccgccca gctgcggcgt gcttcccgtg cgcggattgc ctgcgtggcc
    87181 tcgatgccgc ccatctccgg catctgaatg tccatgaaga tcacgtcaaa ccgccgcttg
    87241 tcatactggg ccagtgcctc gcgcccattc accgccacgg tgaccgtgtg ccccgctttt
    87301 tcgagcagct tgacggcgac cacctgattg acggggttat cctccaccag cagcacggca
    87361 aggcgctctg caatcctcgg ggcggactcc caggcggtct gttcgccctg gaattcgagg
    87421 taatcgacct gctccagtcg gtccacatgc aaggccaggg acaaggcctc ggacagatcg
    87481 cccgccgaga acggtttgat caggcgtgca ccgatgcgca acttctcaca tcggtctgcg
    87541 tcactgcgct gggatttggg atcggagacg accaccaggc gatccagcgt gggccaaccc
    87601 ttgcgcagct gttcggccag ggcaaagcca ccgggcgccg acatggcggc ctccatgatc
    87661 aaccagtcaa acggcttcgc agtatcctgc gcggacttca gctgggcggc cagcgcctcg
    87721 ccatcagcgg cctcccggac cgtcacgccc gccctctcca gctcggcgcg cagcagttcc
    87781 cgcacggcac cgttggcggc cgccaccagg gcccgccggc cggcaaatac cggcggaggc
    87841 cggccgttcg cctggacgcc acgcccgagg cggaccgtga atgaaaacac gcatccacgc
    87901 ccctcctcgc tttccaggct caggtcgccg cccattgcct gaacaatgcg ccgtgaaatc
    87961 gccaggccga ggcccgtacc gccatacttg cgcgtggtgg actcgtctgc ctgggaaaaa
    88021 gccccgaaca cctgctcctg cttgtcggcg ggaatgccaa tgccggtgtc gcgcaccgct
    88081 acgcggagga ggaactcgtt gccatcgagg gactcgccat cgaccgagac caccacctcg
    88141 ccacgctcgg tgaacttgat ggcgttcccg acgagattgg tcagcacctg gcgcacccgc
    88201 gtcggatcgc ccaccaccac cgcaggtacc gcgggtgcaa ccctgtacag gcactcgatc
    88261 cccttctgat acgccgtgag cgccagggac ttcatggtgg cccccatcaa ctccacgacc
    88321 gaaaaatcga tctgctcgag gctcatgcgc cccgcttcga tcttcgagaa atccaggacg
    88381 tcgttgatga tgtgcagcag cgtgtcggcg gagttcttca ccgcgagcag gtagtcccgc
    88441 tgcaccggat cgagatcggt atcgagcacc agatccgtca tgccaatgat gccgttcatg
    88501 ggcgtgcgga tctcgtggct catgttggcc aggaagtcgc tcttggtgcg gttggccgct
    88561 tccgccatct ccttggcatc cagaagctcc agctccgcct ccttgacccg tgtgatgtcg
    88621 cggaaagtcc ccaccagccg gcaccagcgt ccggccgcat tgcggttcga ggcgcggccg
    88681 cgcacctgga tccagcgcca ggccgtggcg ttcgtcgcga tccggaactg ggcgtcaaag
    88741 tcctccagat ccccgcgcag gtgagccgcc agctgatggc gcatcacctg aaggtcgtcg
    88801 ggatgcaccc gatgcagcca cccgtcgaaa tctgcggatc cctcgccggg ggcatagccg
    88861 aggatctgcc gccaatgctc gcccaggacg atcgtgtcgt gtgccagatc ccagtcccac
    88921 agcccggcat cggtgttctg catcgccagg tcgagacgtt cccgtgtctc atggtattga
    88981 tgctcgatcc gctgttttgc ggcaatgagc gcacgaacgc catctaccag ctcggccgga
    89041 ttcgcttcag atgctgccga cggcatggtg atgcaccctc gggacgccag gtcttgcagc
    89101 aggacgcgca gcgcaaggac cagctcgtca ctggaggccg gcgggggtaa ggaggggaga
    89161 ttcactcggg cgatcgatgc gtcacaggat tggcgatggc aggcccgcag cacgcagcgg
    89221 accctgtctc acttcacctt gcgactaacg gacgccctgc ccgaatcttg agatgagggc
    89281 gcccgcgaag gaccgtcagc gcaagccgag cacgtcctgc atgtcgaaca ggccagaatc
    89341 cttgccagca aggaagcgcg cggcccgcag gctgcccgtg gcataggaca tccggctgga
    89401 cgacttgtgg gtgatctcga tacgctcacc gatgcccgcg aagagcacgg tgtgatcgcc
    89461 caccacgtcg ccgccacgga tggtggcgaa tccgatggtc tgactgtccc gctcgcccgt
    89521 cacgccttca cgcccgtaaa tggcgcattc ttcgagattg cggcccaaag cgctggccac
    89581 cacctcaccc atgcgcaggg cggtgccgga aggcgcatcc accttgagtc gatggtgggc
    89641 ctcaatgatc tccacgtcgt agccctcgtt gaggatgcgc gccgccacgt ccagaagctt
    89701 gaagaccgca ttgacgccca ccgccatgtt tggcgcgaac acgaccggaa tgtcctgagc
    89761 tgccgcgcct atggccgcct tgccctccgg accgaagccg gtggtgccga tcaccatggc
    89821 gcaacgggca gcccgggcca gggccaggtg atgcaacgtc ccttcgggcc gggtgaaatc
    89881 gatcaggcag tcggcctcag ccagaccggc cgccacgtcg cagctgatgg ccacaccgca
    89941 ggcctgaccg accagctcgc cggcgtcctt gccgacgaag gggctgccca gctgctcgaa
    90001 cgccgcaacc agcttcatgt cggcagcctt gagggtggtt tcgatcagca tgcgacccat
    90061 gcgcccggac gcaccggcga tggctatacg gacttgactc atgtcatcct cagaaattca
    90121 tgttcaggga accggcagca tgccggtccg tcacggcctg gtcgccgtgc cctcaccgcc
    90181 ggccgcccgc acgtcgccct ccagacgcac cagcttgccg tcctcgaaga ccaccgtcag
    90241 gcgacgctcc tgcgcctcgc ccttgccggg ttgaaggcga tagacgtaat cccagcgatc
    90301 agtatggaag acatcgttca gcagcggcgt acccagaaga aagcgcacct ggtcgcggga
    90361 catgccgggt ttgagctggg acaccgactc ctgggtcaca aaattgccct ggcggacgtc
    90421 gatccggtac ggcttgagaa aatcgatgac cgccgaacac ccaccgaggg tcggcagcac
    90481 aagcagcagg cagagcagag ggcgcatggc ggtcgcggga cgggcccggc tgggccgaat
    90541 tctcaacgaa gggcggaaac tatatcatat ggggctggcc gctcctgcgg gccagcgtgt
    90601 gcacgtaccg gggccaccat gtccagcaat tcccagaacc tcaagaacat cggcctcaag
    90661 gccaccacgc cgcggctcaa gatcctcgac ctcttccaga aggccgacaa tcgtcacctc
    90721 accgcggaag acgtgtaccg tctgctcatc gccgaggaca tggacatcgg cctggccacc
    90781 gtttaccgcg tcctcaccca gttcgagcag gccggcctgc tcgaacgcca ttactttgaa
    90841 tccggcaagg ccgtcttcga gctcaacgaa ggccatcatc acgaccacct cgtctgcctg
    90901 cagtgtggca aggttgaaga attctacgac ccggaaatcg aggagcggca gaaccgcatc
    90961 gcccgcgagc gcggctacga ggttcatgaa cacgccctct atctctacgt ggactgcaag
    91021 aagactgatt gcccccacag gcccggcaaa tccggaacac ccctgatctg accctccggc
    91081 ggcactccgc cgccctctcc atggaagcct tcctcacctc cgcggcgctc gtcgcccttg
    91141 ccgagatcgg cgacaagacg cagcttctgt ccttcgtcct cgccgcgcgg ctgcgcaagc
    91201 cctgggccat cgtcgccgga atcttcgtcg ccacaatcct caaccacgcc ctggccggct
    91261 cggtgggcgt atggctggcc aaccttattc cggcccactg gctgccctgg atcgtggggt
    91321 ccgtgttcgt cgtcttcggc ctgtggaccc tcaagcccga caccctggac gacgatgaag
    91381 cccctgccgc catgcacccg gccggcgcct tcatgaccac tctgattgcc ttcttcatgg
    91441 ctgagatggg agacaagacc cagctcgcaa ccgtcgccct ggcggcgcgc ttcgatgcac
    91501 tgggctgggt cgtcctcggg accacgatcg ggatgatgat cgccaatgtg ccggcagtgc
    91561 tgctcggcga agccctcgcc cgccgcctgc ccctcaccca gatccgctgg gccgcagcgg
    91621 ccgtgttcat cctgaccggc ctggtcacct tcgccggcgc ggccggtctc gctggctgat
    91681 ccggcgacaa ccgcgggaat cagaccggat tgcctgccgc ggcgagcatt tccgctgcgt
    91741 ggctgcgggt ggtatcggtg atctgaacgc cccccagcat gcgggcgatc tcttcgatgc
    91801 gctcggtcgg cgtcaacacc atcacccgtg acagggtctc gtcgccgtcg ttatccttgg
    91861 caatgctcca ctgccagttg gcctgggctg ccacctgagg caggtgggtc acgcacagca
    91921 cctgacggtc gctgcccagc tggcgcagga gccgccccac aatctccgcc acgcgaccgc
    91981 caatgcccac gtccacctcg tcgaagagca gcgtgggcgt ggcagcgttg cggctggtga
    92041 tgacctggat cgccagcccg atgcgtgaca gttcgccgcc cgaggccacc tttgcgagcg
    92101 ggcgcagcgg ctgactggca ttggccgtga ccaggaattc aacccgctca agccccgtcg
    92161 cagcccccgc gacctctgtc gccaggcaca cctcgaaacg ggcaccggcc atcgccaggg
    92221 tctgcatcgc ctccgtcacc gctgccgaca gcgccgacgc cacctgcttg cgcgccaccg
    92281 acaagggccg cgccaccgcg aggaaagcct cctgcgccgc cgcctcttcg cgggcgaggg
    92341 cctccgggtc ggactgccgg gtcagctggg cgagccgcgc gtcgctctcg gccagcaggg
    92401 cgggaaggtc cgccggtgca caacgatgct tgcgcgccag ggccgtcact gcctgaatgc
    92461 gctgatccac ttcggtcagg cgctgcggat ccagatcgag ccgatcccga tagcgccgca
    92521 gggcatgcac ggcttcatcg agctggatgc cggcaccccg cagaagatcc tccacatcgc
    92581 ccacgcgggc gtcgtactgg cgcatgtccg ccgcccggcc ggccagccgc tccagcagcg
    92641 acaccagggc gagctcacct tccgcaaggc cgtccaggat ctccccgcta ccttcgatca
    92701 ggctggccgc gtgagccagc ctggcctgtt cctggttgag ggcgtcccat tcatcgggtt
    92761 cgaatgccag ttcccgcagc tcggtcacct gccaggtgag cagttcgcgc tcccgttcgc
    92821 tcgaggcggc gcctgcctcg cttgcttccc gagccttgcg ggcccccatc cagacacgga
    92881 agcgacgggc cacttcaacc gcatcggcac tcaggccgcc atgggcatcg accagcgcca
    92941 gctgggcctc acttcgcaac agggcgtggt gggcatgctg gccgtggatg tcggcgagca
    93001 gctcgccggc cgctcgcaac tgcccgagca ccaccggcga tccgttcagc caggccttgg
    93061 aacgtccgct gctgtcaatg acccggcgca tctggacggt gccgtcctcg gacgtgagcg
    93121 actgttcttc cagccaggca gctagcgcac tgtcggcggg aagatcgaat tccgccacga
    93181 tctctgcgcg cgcctgaccg ctgcgcacga cgccggcgtc gcccttgcca ccgagcgcca
    93241 gacccagcgc gtcgagcagg atggacttgc cggcacccgt ttcgccggtg agggcgccaa
    93301 agccggcaca gaactccagc tcgaggtgat cgacgatgac gaaatcgcgg acagtcagat
    93361 ggcgcagcat gggcagacgg cgagcgcgct caactgtggc gcggcgtgga actccagtgc
    93421 agcttttcgc gaagcatcgc gaaatagctg tagcccacgg gatggagcag gcgcaccgtg
    93481 tggggcgaac gggtgatccg tacggtgtcg ccggcgcggg cgtcatagcg gctctggccg
    93541 tcaaagtgca cgcgcgaatc gtggggcggc agcaggacga tctcgatgca gcagcggtcc
    93601 ggcagggtga tcggccgcgc cgtgagggca tggggacaca tgggcacgag ggcaatgccg
    93661 tccacactgg gatgcaggat cggcccgttg gctgacagcg cgtaggcggt ggaaccggtc
    93721 ggcgtggaga cgatcatccc gtccgaacgc tgggtgtaga cgaattcccc gtccacccgc
    93781 aggtcgaatt cgatcattcg ccccagctcg cccttattga ccaccacatc gttgagcacc
    93841 gtggtgcgga atacccgctg gccgccccgc aggacttcca cgtccagcag cacgcgatgc
    93901 tcttcggtga aacgccccgc gaggatctcg ccgatcttgt cgagggccac atcgcgggag
    93961 atgtcggtca ggaagccgag ccgcccctga ttgacgccca ccagcgggac atcgtagccg
    94021 gccaggcggc aggcgctgtg caggagcgtc ccgtctccgc cgagcaccac cgccagatcg
    94081 gcccgcacgc cgatctcttc ataactcccc accgtgaaac cggaggtcaa tcccgtcgcg
    94141 ctggcagtgc cctgttcgat gatcaccggc agccccaggc cgcgcaggta attggccagc
    94201 cgcagcacgg cttccgccac ttccgggctc tggtatttgc cgatcagggc gatagtgtcg
    94261 aatgcatggg ccatgcaacg attaaaccac atattcggcg aaacgcgctg ccattcaccg
    94321 gcatgtgcga gaacgtatca ccatacggaa cgccccgcct cacccctcgg aactgcgtgc
    94381 cgaatgggcc ctccagcgcg ttgcccttct cagcaccctc ggccttcctt agaatccatc
    94441 gcgtcacccc cgaatttccc gccatgagca gcctcgatca acgttcacag atcctcctca
    94501 agaccctggt ggagcattac atcgccgacg gccagccggt gggctcgcgg gcgctgtcgc
    94561 gcttttccgg cctgcagctg tcgcccgcga cggtgcgcaa cgtgatgtcg gatctggagg
    94621 aactgggctt tatcaccagc ccccacacct ccgccgggcg gattcccacc gcgcggggct
    94681 accggttctt cgtggacagc ctgctcaagg tcagtccggt cggtagcggt cagatcgaaa
    94741 ccctgcgtga ccagatccag cccggcgaac cccagcgggt catcaacgcc gcgtcacagc
    94801 tgctttccga cctcacccac tttgccggcg tggtcatctc gccgcgccag gaatccaccc
    94861 gcatccgtca gatcgaattc ctgcgcctgt ccgagggccg catcctgctg atcgtcgtca
    94921 ccgccacagg cgacgtgcag aaccgcatcc tgttcaccaa acgcagctat tccccctccg
    94981 aactggtcac ggcggccaat tacctcaatc agcacttcgc gggcctttct tttgacgaga
    95041 tccgcatccg cattcaggac gagctcaaac agctgcgctc cgacatgacc gagctgatga
    95101 ccgccgccgt cgaagcgggc agtgcagcgg ttgccgagca ccagcagacc tacgtcatct
    95161 cgggcgagac caacctgctc gacgcggagg acctgtcctc caacatggcc cgcctgcggg
    95221 aactcttcaa gctcttcgag cagaagaccg gcctcatgca actgctcgac atctccaacc
    95281 gcgcccaggg cgtgcagatc ttcatcggca gcgaagccgg cctgtcgccc ctagacgact
    95341 gcagcgtggt cactgcaccc tacgaagtgg acggccaact cgtcggctcg gtgggcgtga
    95401 tcggccccac gcgcatggcc tacgaacggg tcatccccat cgtggacatc accgcccgtc
    95461 tgctcaccaa cgccctttcg ggtcagaact aggaatcctt catgaccaga ttctggcagg
    95521 agccgccctt ccggcatggc tccccaagcc gcacgggcat cctcatcgtc aatctgggca
    95581 cgcctgcagc ccccactgcg gacgcgctgc ggccctacct gcgcgagttt ctgtccgacc
    95641 cacgggtcgt ggaaatcccg cgaccgatct ggtggctgat cctcaacggc atcatcctca
    95701 acacccgccc cgccaagtcc gcccgcaaat acgccagcgt gtggaccgac gagggctcgc
    95761 ccctcaaggt gcataccgag cgtcaggcca aactgctggc cggcttcttc gtggccgacg
    95821 gcgcacccga tctcgtcgtg aactgggcca tgcgctacgg ccaaccctct gttcgcagtc
    95881 gcctggatga aatgcgcgcc cagggctgta cccgcgtcct ggtgattccc atgtaccccc
    95941 agtacgctgc cagcaccaca gccagcgtgc tggatgccgt cggcgactgc ctgcagcact
    96001 ggcgcaatct gcctgagatc cgctacgtac gcagcttcgc ggacgaccct ggatacatcg
    96061 gcgccctggc tgcgtccgtg cgggaacact ggcaacgcaa cggccaggcg gaaaagctcg
    96121 tcctgtcctt ccacggcatc ccgcggcgca gcctcgacct gggcgacccc tatcactgcg
    96181 agtgccacaa gaccgcccgc ctgctgggtg aagccctgaa gctgccagcc gagcgcctcg
    96241 tgatcagttt ccagtcccgc ttcggtcgcg cgaaatggct ggagccctat acccaggcta
    96301 cgctggaggc attggcgcgg gacggcgtga cctcagtgga tgtgatgtgc cccggattca
    96361 ctgccgattg tctggaaacc ctcgaggaga tcaacatgga atgccgcgac gccttcctgg
    96421 ccgcgggcgg caaggccttc ggctacattc cctgcctcaa cgaacgggac gactggatcc
    96481 gcgcgctccg caccatcgca cgccgtcacc tgggtcactg gctcgatctg ccagcggagg
    96541 atcccgcgcg gcttgccgca tccgcggaac gcgcccgtca gctcggcgcg accatctgat
    96601 ggcgcggacc tgacacctga aaggacctct taatgaaact gatcctgcgc tggtttctca
    96661 atgcagtcgc cttgatgctg atcccggaag tggtcacagg gatccacgtc gacagctact
    96721 ctgcggcgct catcagtgca gtgctgctgg gcttcgtgaa tgcgatcctg cgaccgcttc
    96781 tgttgctgat caccctgccc atcaccgtgt tgacgctggg cctgtttgcg ttggtgatca
    96841 atggcctgat gttctggctg gtctccggcc tggtgcaggg cttcacggtc acggacttcg
    96901 gcaccgcctt cctgggtgcc ctgctctaca gcctcctcac ctggctggtc aatctcgccc
    96961 tcgggggaga ctgacggacc aggatccggt tctgtcgatc agttgcgtga cgcgggagcg
    97021 cctccggtag ccggaaccgg cgtggtgcgg cgccgggagg acttgcgacg cacggcaccg
    97081 ggatcgaatg cgccctcgag cttttcaagc gccatcgcgc gcgcatccat cgccatgcgg
    97141 aatgcgccat cctcaccgta cttcttcacc gaaaacttga cacgcttgcg gcgcccgtcc
    97201 ggcagaggcc aggaggccac ccagaaccag cgctgctttt cctccggcag gtcgcgggtt
    97261 tccgacgcgc aatagcggca cacccccacc acccccgaac ggttgttctt cttgacgatg
    97321 ttggagtact cctgcatcga caggggcgga tgcgcggcga tgatctggtc gcggtattcc
    97381 tttgcggcag aaaaggaacg ctgcttgccg ccgaacacgc cgtcactgaa atgcttgcgg
    97441 tagatcacgc cgcgacgctg aatcgtcacc agccagccgt gggtgcgact cgtgtcattg
    97501 tccacccggc tgattccata agtgctgcct cggctcacag gccacctcct gtcgactccg
    97561 cgatccacac gcggctacgc caggaatgtc ggcgcggcaa ggcaaaactt taggttgaaa
    97621 ccggcacgat ccgccccaca tgagggacat cctgctcctg gagcctgcca tgcctgatcc
    97681 gatcctcgtc ccctgccccc attgtggagc gatcaatcgc ctgccgggcg aacgtctgcg
    97741 cgaccagggc cgctgcggac ggtgcaagca ggcgctgttc acgggcgaac ccttgcatct
    97801 gacgggcgag gatttctgga ttcatgccgg tcgcggtgaa atccccctgg tcgtggattt
    97861 ctgggccccc tggtgcggcc cctgtcgcag catggaagcg tcgttcgcag ccgcggcccg
    97921 gcgactcgaa ccggcctacc ggctgggcaa agtcaatact gaggacgaac ccgaccttgc
    97981 cacccgcttc gggattcgaa gcattccgac gctggtgctg atgcggggcg gacgggaggt
    98041 ggcgcgccga tccggcgcga tcggtgcaga tgaactggtg cgttggctga caagcgtgtc
    98101 gtgatcaagg ccggcagcgc cggacaagca cgtgctcagc tgtccaccag ctggagttcc
    98161 ataccaaacc cactggcggt gatgcgctca gccggcggtc cgtctttgga aagctcccgc
    98221 atcgaatttc ccaggcgacg ggccgccacc gtgaccacct tgctgcggac cgcgtcctga
    98281 cattcctcgg tcgcaaggga cagcgccgcg ggattcattt ccaggtaggt cagtgatgtc
    98341 gtcgaaacga cggtgtacat gtgcttgtgg tcctgctgat cgaggaacgc cagttcgccg
    98401 aacagctcgc cgtcgccaag ttcgcagacc cgatggccgt ctaccgaaag ctcgaccttg
    98461 ccatccacga tgatgccgaa gcgctggtcg gcatcgcctt cgcgcatcag gagcgtgttc
    98521 gaatccaccc tgcgccagcg gctgatgcgc agcacctccc agatctcgac atcgtcgaat
    98581 tccaggaaga aggacatctt gcgcaactgg gagaagcggc ggttatccgg cggaacgtag
    98641 gagtcgtcaa agatcttgta gcggacagcc gacagatcct tcgcgaaatc cgcgccgttc
    98701 ttgtagcgcg agtagagatc cttctcgagt gacttgcgga tcacggcatc catctgctcc
    98761 gggatttccg gattgatctg ggacaccagc ggcggttcgg cgttgatgat cttgtagatc
    98821 agctgggcgg gattcttggc gcggaacggc aggcgcccag tcagcatgtg gaacagcacc
    98881 acgcccagag aaaacagatc ggtcttctga ttgagcgggt aacccttgat ctgctccggg
    98941 ctcatgtaag ccggtgagcc cacacccatg atgaaggtgg aatcggacgc gctcttcttg
    99001 ctgatgttga gcgccagacc aaagtcggtg atcttgacgt tgtcgttctc gtcgacaagg
    99061 atgttggccg gcttgatgtc ccggtgcacg ataccctggt gataggcgta atccagtgcc
    99121 atgcaacact tgaacaccat gccgatcgta cggtggagcg gcaacaggcg ctcgaagcta
    99181 cagtagtgct caagcgtcgt tcccggaaaa tactccatca cgatgtaggc gaagtccttg
    99241 tcgaccgccg tatcgaagat cttgatgatg ttggggtgat cgagacggcg ggagacgtcc
    99301 atctctgcgc gcagcagttt gagcaggcgc cggttccatt tggcctcatc cttggacttg
    99361 tcgtcaaagc ggatgagctt gagggccacg ggatccgggt actggggatg ctcagccaga
    99421 tagacggtcg ctgtcgcgcc ccgcccgatc tcccggagaa gcttgtattt gccgaccttg
    99481 tcaggagcct tgtccatacc tgcccgtcac gaatacaccc aaaagaatga ccggtacccc
    99541 attatcggac aggattttac accccattgc agcactttag gcacttccgc ccctcttgaa
    99601 ataccgcttg ccgcccccag ctacgtgcgg tcaacactgg gagccatcat caatgcaaga
    99661 ccaaaccacc ccgccgaccc ccgaccacgc accggaactc gtccagggca gcgaccttcc
    99721 ccccgcggca cctgacgcgc cggaagcccc tgtggacgtg gtcccgggcc ttgaacaagc
    99781 cctccgccag gccgaactga aggcggccga acaccacgac gcctggttgc gcgcccgcgc
    99841 cgagacggaa aacgtccgcc gtcgtgccca ggacgacatc gccaaggcgg gcaagttcgc
    99901 cgccgaaaaa ttcgctcagg cgatgttgcc cgtcaaagac agtctggagg ccgccctggc
    99961 cagcgagaac gccaccctcg aaagcctccg gcaaggcgtc gaactgaccc tcaagcaact
   100021 cctgtcggcc ttccagggcg ccagcgtgac cgaactcaac cccctcggcg agaaattcga
   100081 ccccaaccgc caccaggcca tcagcgccat cgaggcggac ggcgagccca ataccgtcat
   100141 caacgtgctg cagaagggct acctcctcca cgaccgcgtg ctgcgtcccg cgctggtggt
   100201 ggtgtccaag gcgcgcagtg cacaaccggc tgcttgaaag cgccggacgc atccccatat
   100261 ccgaaccaag ctggtcgcaa ccctctcccc tggcgcggcc cctcgaacag cattccaact
   100321 gaaggaactc tcatgggcaa gatcatcggt atcgacctcg gcaccaccaa cagctgcgtt
   100381 tccgtgatgg aagccggcaa gcccaaggtc atcgaaaact cggaaggtgc gcgcaccacg
   100441 ccgtccatcg tcgcctacgc cgacgacggc gaaatcctgg tcggcgcgcc cgccaagcgt
   100501 caggcggtca ccaacgcgcg gaacaccctc tacgcggtca agcgcctgat cggccgccgc
   100561 ttcgaagaga aggaagtgca gaaggacatc gacctgatgc ccttcaccat cgccaaggcc
   100621 gacaacggcg acgcctgggt ggaagtgcgc ggcaagaaga tcgccccgcc ccaggtctcc
   100681 gccgaagtgc tgcgcaagat gaagaagacc gccgaagact acctcggcga ggaagtcacc
   100741 gaggccgtca tcaccgtgcc cgcctacttc aacgacagcc agcgtcaggc caccaaggac
   100801 gccggccgca tcgccggcct gga
//
LOCUS       DAOQPA010000002        78172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_98_length_78172_cov_19.060038, whole genome shotgun sequence.
ACCESSION   DAOQPA010000002 DAOQPA010000000
VERSION     DAOQPA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 78172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78172
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_78172_cov_19.060038"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..880)
                     /locus_tag="PKX01_00485"
     CDS             complement(188..880)
                     /locus_tag="PKX01_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HMZ74626.1"
                     /translation="MAHAPRIQAVAFDAFGTLFDVYSVGLLAEQLYPGRGAQLADLWR
                     QKQIEYSFIRTLSDRYKPFWSVTEDGLVYAAARLGLELDAPRRTQLMNQYACLSPFPE
                     NLGALKALKEAGVPTAVLSNGTPEMLDVAIKSAGMNGLFDHVLSVDAVQQYKTSADAY
                     ALGPAAFGCPAERILFVSSNGWDAAGATWYGYTTFWINRSGLPQEQLDVSPVARGRQL
                     SDVVDYVARHRA"
     gene            1061..1966
                     /locus_tag="PKX01_00490"
     CDS             1061..1966
                     /locus_tag="PKX01_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ74627.1"
                     /translation="MSNFKEIEAFVSVAARGSLSAAARADGVTPAMMGRRIDALEERL
                     GVKLLVRTTRKLSLTFEGSAFLEDCQRILNDLANAEASVSLGGVKASGHIKVSAPAGF
                     GRRHVAPLVRDFLAAHGDVSCSLDLSDRLVDLVNEGVDCAIRIGDLSDSSLVSVRLAD
                     NRRVVVASPAYLALHGVPATPEDLLSHDCLSLNPQRGWVFCDPDAPGTTRVVKVSGRF
                     ECNDGTVLHEWALAGIGLAWRSMWEVQRDLAQGALVSVLDAFAAPSTGIYAVFPQNRR
                     LPLRVRLLIDHLRGSFGQPDYWRAG"
     gene            complement(1948..2430)
                     /locus_tag="PKX01_00495"
     CDS             complement(1948..2430)
                     /locus_tag="PKX01_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74628.1"
                     /translation="MVQVTFTPAIAERRDDALSLAPAPADPPEPPPVEQPPTPGADDT
                     PPSADALPLSALSSPPDLLTPVPASIWPPFPELGHRIFQLEVIVAADGKVAAVNTQCD
                     EALCAAADAYAAIVRTWEFTPGEYRKHPVPVRLRIEFELNPPPPETPTESSPTQPARQ
                     "
     gene            2719..3162
                     /locus_tag="PKX01_00500"
     CDS             2719..3162
                     /locus_tag="PKX01_00500"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HMZ74629.1"
                     /translation="MKRQSGVTLIELLIVVVIVAILGGIAVPAYTDHILRGKIAQVTE
                     NLAEAKVRMEQVFNSNRTYATSPGGTSCPDFSTLFDKTGFTLKMSDCPGDAGTTFTLT
                     ATGSSSSGTGGFMYSINQNGEKKSTTPATSEVQSCWLMSKTASSC"
     gene            3156..3773
                     /locus_tag="PKX01_00505"
     CDS             3156..3773
                     /locus_tag="PKX01_00505"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HMZ74630.1"
                     /translation="MLIPARRLRGATLLELMVTVSIVAILMAVGMPSMKDWILRNSVS
                     SAAEVLQNGLRRAESEAIRRNAPIEFLLTSGIPSSSASLTAEANAVNWAIRVVDSSNS
                     YKPVAGGYVAGFYLKDISSAVTVSGPASVIFNGMARVLDSAGVPVNGYQVYRFSRSGS
                     DKAYCVFVSQGGGVKMCDPDQASGTPRACQPLLSASQCPKAGSGS"
     gene            3770..4198
                     /locus_tag="PKX01_00510"
     CDS             3770..4198
                     /locus_tag="PKX01_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74631.1"
                     /translation="MKRRSFAGYVLLEALVGLLIFSLGLLGLIGFQASAMKIASDSRF
                     RTEAAMLADELIGKMSMSKISTVQTEYSADGSKFSDWLNNRVKGGSKLPGAAASVTFA
                     TANTNATLLATVTISWDLPGSSGRVGDSLHGVYVTQAMLF"
     gene            4211..5446
                     /locus_tag="PKX01_00515"
     CDS             4211..5446
                     /locus_tag="PKX01_00515"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HMZ74632.1"
                     /translation="MRRPPFPVLRDRSRQRGISLVETMIGMALGLVATFVIIQSFSSS
                     ETYRRNLAGTADATQTAAIAGGHLDLVLQEAGASLVQGRNVWGCKILVTRNKTAILPH
                     GSNYPSPFASFPTTVRVLPLAVLDGGDKGSDVIMAMVGNSGTGNRDTPFSAPAGGKSM
                     VVSNNNGMALTSSGQSPNDLFLSVPQEVAGAPGDCQVVQADSSFSNGTAVADPSLGLQ
                     VMPASVTAPQSLTTIPLNASTYGALASSANSPSAFHLGRESAPIFSLFAVNSNGELVE
                     YDLLERNGVMTFGENIFLIKARYGLDNGANGGTANDNVIDDWVSPAEDGWKLAQLMDG
                     KSSTEQKVDQIKAVRLALVTRSSQSVTSDAKVSSLTLFSDLSSTRQYTRSLSDSEQAY
                     QYQIVDWVIPLRNMKATPK"
     gene            5458..6111
                     /locus_tag="PKX01_00520"
     CDS             5458..6111
                     /locus_tag="PKX01_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74633.1"
                     /translation="MTTSKIPLHPPRRNRGAVLAIVLVVLVVMMLGAVSMLRSVDTSA
                     LLSGNLAFKRESLNRSTVGLNTAFAQFKKDDFVKYSDDSTGCNAASTGPSCTYASNWL
                     KMNYSPRLLATDGNGVPLILKDISSFDKTFNSSLVVTDKADPNVKVRFVIERMCNDFG
                     PATETKCILSENAERGGSQPLDKLGSAALPMYRITVRSDGVRNSQTYAQAVVTTRVK"
     gene            6138..10001
                     /locus_tag="PKX01_00525"
     CDS             6138..10001
                     /locus_tag="PKX01_00525"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HMZ74634.1"
                     /translation="MKLSKSLSRFLLIVASASALFSAGLQDGRAASTPLADQPLLTAQ
                     VPSNVILALSVEFPTALSVAYNTKFSVDGSAYLGYFDPKKCYKYDSANNYFVPQGPSG
                     TNYTCTSAWSGHFLNWATMQAIDTFRWAMTGGNRVIDLPVNFTDTQPVKTVLQRAYAS
                     SDGSECTYFPGRGISASDRANYTDTAGVGYTDKGLWIRNGGQGYQFQVAPQIGGAANC
                     SSWGGGSGSVQSYYAQVEVCKNANGSDGKTLLESNCTMYVDPTGTKTAYRPIGLMQQY
                     KLAMNFGAFGYLYDGSSTRDGGVLRALSRPIDLEISDTGAFYPDPFGLANASRGIIYS
                     GTANYLNKFGSTSLHYKSLDPVSEMYAEVMKYLKGMDVTPSYVSTINNSMRDGFPVFG
                     ESGGPTWVDPARDTSDPSYPNSPALWCKKSYIIGIGDTNTWNDQNLSGRTDVSVPSGV
                     DTNLAGGTAKSWTDTVGTTEGYVPSGYASLGVAQPNPGHSNSFLMAGIAYYAHSSDIR
                     TDLKDTQSVDTYWMDVMEGGIYKDKNQFWLAAKYGGYKKPLNTAMGPFNSSTDFWKSQ
                     GKVIQTKSDGTVVTTGGAKSYDLPDNYFPANNPANMVSSLQSAFAQISAGTGAAAGVA
                     LSNPQVNAASGGSMTFQGTYDASTWSGNVTASNITSFKDDGTPVTTSVWDAAARLNSM
                     VASGGYSSKRKVVTLVPTKVDNATPAVADLVGKPFLYANLSTAQKLNLSKTSLGGDLG
                     DGDKVLNYLRGDRTYETTATTPNLYRLRGSVLGDIIDSKIVYVAAPNAVYSDSYNPGY
                     ETFKKNAASRQPVVYVGANDGMLHAFDATSASTGGDELFALVPYALYAGPSNTPDVDG
                     LQAIARTSYSHHYYVDSTPEVRDVDFMRAGGATQSDATKSDWRTLLVAGLGKGGKSFV
                     AMDVTSISSGLTESQVAGKVLWEFFHPDMGFSFGRPLITKTRKWGWVVILSGGYNNIG
                     GDTKAGYKPGQGVVFVVSPKDGSLLQAIYTGAGDAASPAGLAQITGYTQNYQDYTTDY
                     VYGGDLSGNLWRFDFTDSSKSVPSPTKIAQFKDPDGTPQPITVAPKAEYSADDLKRYV
                     FVGTGRLLASSDQKNAQKQTMYALRDGTRTYAFGSNTNQVALPDGVSFPVGRGSMSGV
                     SDLLAGATKSESTPMGWYYDLTGVTSGVTERVVLALQANDGVLSWVGSLPNSDPCSPS
                     GASRLYSVQYGSGQSVFYELVNGLRSPVAYLSIDQGLADITLVRYGSSIRVVGTDTKG
                     GAKVYGNTIAESSDPRVVNWRIIRE"
     gene            10063..10138
                     /locus_tag="PKX01_00530"
     tRNA            10063..10138
                     /locus_tag="PKX01_00530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10096..10098,aa:Thr,seq:cgt)
     gene            10281..12755
                     /locus_tag="PKX01_00535"
     CDS             10281..12755
                     /locus_tag="PKX01_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ74635.1"
                     /translation="MSHSVPPQEHACYHCGLPIPSDVHLGVTVAGVERSMCCTGCQAV
                     AQSIVDGGLVDYYQRRDAMPEPVREAMPDELRDLGLFDHPDFQQGFVKPVGEHEREAS
                     LILEGITCAACIWLNEQHVARQPGVMAVEINYATRRARVRWDERRIKLSNILAAIQAI
                     GYRAYPYDAARSEQISQKERRAALWRLFVAGFGMMQVMMYAVPVYMAGEGEMTADIEA
                     MMRWASLILTLPVVLYSAAPFFRNALRDIRLRRLGMDVPVALGVGAASVASCWATFAG
                     HGEVYFDSVTMFVFFLLCGRYLEMLARQRAIRGVEELGKALPAFAERLTAFPSLEGER
                     VPVSQIVSGEVLRVRPGETIPVDGVVIEGTSEANEALLTGESRPVPKGPGVDVTGGSV
                     NVSSPLLISVTQTGEHTRLAAIRQLMERASAEKPQVVQQADKVAAYFVVALLVLAFAT
                     AVGWWWFDPDRALWVFVSVLVVSCPCALSLATPVALTVATDALARVGVLVTRGHAIEA
                     LAKTEQMVFDKTGTLTYGDMRVEATCLLGSHSTRDVQRLAAALEQGSEHAIAHSLRKL
                     VTDAPDQLPVLSDVTVVTGQGVGGRLDSERVRIGRPEFVAEICPGTLPAELTSAQASG
                     KTVIALGGESGWLAGFFLSDVLREDAHDVFDALHGDGVTTAIFSGDTQGVVSELGKAL
                     GVTEAVGELTPEGKHVRLSALQAHGKVVAMVGDGVNDAPVLAQAQVSIAMGGGTDLAR
                     NQADIVLLSGRLGSLVDGMRLSRRALRIIHQNLWWSFAYNFTAVPLAMSGFVTPWMAG
                     LGMSASSLLVVLNALRLQRKHGKVAA"
     gene            12755..12952
                     /gene="ccoS"
                     /locus_tag="PKX01_00540"
     CDS             12755..12952
                     /gene="ccoS"
                     /locus_tag="PKX01_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMZ74636.1"
                     /translation="MESLYLLIPLSIVLVFVIGVVFWWSVRSGQFDDLEGPGFRVVTD
                     DDTPRTETESPVERKGTTERS"
     gene            13641..13964
                     /locus_tag="PKX01_00545"
     CDS             13641..13964
                     /locus_tag="PKX01_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ74637.1"
                     /translation="MGNLAYSVDKTRLEEGFREFGTVVSAQVIMDRETGRSKGFGFVE
                     MGSDGEAQAAISAMNGKSFDGRNLTVNEARPQEPRSGGFGGGMRRSGPGGGRRGPTSG
                     GGDRY"
     gene            complement(14121..15812)
                     /gene="recJ"
                     /locus_tag="PKX01_00550"
     CDS             complement(14121..15812)
                     /gene="recJ"
                     /locus_tag="PKX01_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HMZ74638.1"
                     /translation="MTRILSRSAPGQAVTALLDTGVHPLLARIYAARGIATRHELDYG
                     LQHLLPPNQLKGVGAAAQLLADAIEANARMVIVADYDCDGATACAVGMRALRAFGADV
                     HYIVPNRFEYGYGLTPAIVELAAALQPELIITVDNGIASVDGVAAARECGIATLITDH
                     HLPGDELPEADVIVNPNQPGCEFPSKALAGVGVMFYTMLALRAELRRRGAFDGQAEPK
                     LADLLDLVALGTVADVVRLDHNNRILVAQGLQRMRAGRMQPGLRALFAAAGRDAGRAT
                     TFDLGFALGPRLNAAGRLADMSLGIECLITDDASRALQITQELDALNRERRAIEADMQ
                     EGALAGLDGLSPGDCSSLVLFEPDWHQGVIGILAGRIKERFHRPTIAFARSHEGELKG
                     SGRSIPGLHLRDALDLVTKQAPDLILKFGGHAMAAGLTIREPDLGPFRDAFERVVRQM
                     IDPADLTRSVETDGSLEEGFYSLETARLLENQIWGQGFPAPLFDDCFRVENQRLLKDK
                     HLKLQLRKGALRIDAIRFNHADPAGAAIHAAFRLAVNEYNGVASVQLMVEEFENT"
     gene            complement(15809..16858)
                     /locus_tag="PKX01_00555"
     CDS             complement(15809..16858)
                     /locus_tag="PKX01_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74639.1"
                     /translation="MQYDLVLPGLVWPASGLAGPAADLELPALAALIGHARVHWGAAC
                     TPEAWLADRFQLAGKRIPYAWLRRLGEATTSPERSAHWLCIDPVHLHFARDKLLLADA
                     SGLDITQAEADTLIAGLNETFADIGRFEAPAPDRWYLRAEREPRPFFHPLADVTGRPI
                     QLFLPEGDDIPFWARLSNELQVWLFNHPVNAAREAAGQRAINSAWLWGAGQPLTPIAP
                     APSLQADDPYTCGLIRASGGAPRSSGPYAAPGTDGFTLLTALRRPLLHRDPDAWRATL
                     GTIERDWFAPLLAALRSRHLSRLRITAPGDRACLELDVTAASLWKFWRKPLALDTLLR
                     QTPNPASDVASTTAP"
     gene            complement(16942..18501)
                     /locus_tag="PKX01_00560"
     CDS             complement(16942..18501)
                     /locus_tag="PKX01_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ74640.1"
                     /translation="MTRFHPPRFPAFHRAFATALLLATGIVHAVGATVIHDDALRAQP
                     RSDAAIVMPLHRLSSVDIADARRDWYLVDAAGNRRGWVPAASLRLDEPPASPSAPAAP
                     TAFAASGARPRLQPHASRHALLLDLTDHDPVPPGSHATDLTARQLARALGVPDANIQQ
                     PDPVSLTPDRIRRALADLDAHAGPGDRVFVHILARTEPPRAARAPSAAALATPDGRVQ
                     VAELGRYLDTLARKAGQVLIVVEAASPAPQTMAPDRRTDVPSRSHGLLQHWQEALPNS
                     PPNLALLASDSPDFGAASAALLACLSGDAPVTSTSGLARIDDLRRCAEAQLGPARPGA
                     RRLERVGNADLVPLPTLTPAAAPGFTPAALLRILHAQRAADRPLDVRRQDDRLLIRSP
                     RAGFLYVLHAPSDGRGFELLLPDASDSRLHLAPGVQISPSLAGTPDGPLLVLVTDAPR
                     RFVRAGFQAAGAILATPIDARSLRDLPAEVLEGDNDPRCLLAETRNLGPEQALRCSAA
                     YASALIEPRQP"
     gene            18665..19918
                     /locus_tag="PKX01_00565"
     CDS             18665..19918
                     /locus_tag="PKX01_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HMZ74641.1"
                     /translation="MSYEFLVGLRYTRSKRRARKGNRFISFISLVSMAGIALGVAALI
                     VVLSVMNGFQRELRTRILGVASHVQVTGFDGELANWQRIADEASKHPRVLAAAPFVQA
                     QGMLSFDQTVRGTLVRGILPDAEDKVADFAQHMRAGRLDELQPGGFGIVLGADLARAL
                     RVTVGDKVTLIAPQGLVTPAAILPRLKQFRVTGIFEVGMYEYDSSLALVHLQDAQALY
                     RMEDRVSGVRLKLDDLFKAPQVSRELMGYVQADTLISDWTRAHANFFRAVQIEKNVMF
                     IILLLIVAVAAFNIVSTLVMAVQDKRADIAILRTLGASPGSIMGIFVIQGALIGVVGL
                     AIGVIGGVALALNIDVVVPAIERTFGIQFLAKDVYYISDLPSQLLWSDVGVIATVSFV
                     LTLVATLYPSWRASRLNPAEALRYE"
     gene            19911..20606
                     /gene="lolD"
                     /locus_tag="PKX01_00570"
     CDS             19911..20606
                     /gene="lolD"
                     /locus_tag="PKX01_00570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HMZ74642.1"
                     /translation="MNERTSDGVIACRGLAKTFREGDDAVSVLTDVTLSVQRGERVAV
                     VGASGSGKSTLLHLLGGLDVPSAGSVSLLGRDFSTISDAERGRLRNQSLGFVYQFHHL
                     LAEFSALENVAMPLLVRRLPREEALARAAEALKDVGLEHRMDHTPGELSGGERQRAAI
                     ARALVTRPTCVLADEPTGNLDRQTAERVFGLMLALAEQHGTSFVIVTHDTALAARTHR
                     VLHLRDGRLAGET"
     gene            20746..22767
                     /locus_tag="PKX01_00575"
     CDS             20746..22767
                     /locus_tag="PKX01_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ74643.1"
                     /translation="MHLLFAPAVALLNRMRYRAKFALLGALAFAVVLFLLGQLAFSLR
                     ESMHFTEAERSALGVVPRLLTVIQLTQQHRGLSAGVLNGNEGMRIRLTAKTGELGEAM
                     KKVDAIALGADASIPMARWGEVKSAWEVLRANGLNLPPKENLTAHSRLVAMEVLALHD
                     LGDDGNLTLDPVADTYYLIDNLLKRVPEVTERLGRLRALGTGVLAAKSLDEQKRYDIS
                     VQLGELDMAVEALNENFDRAIRFNPALKGSLGALRDGFNAGTGKVLDQLQNHVLKGDF
                     EMAPQAYFDLVTQAIDIAYAKTFGELIPATDELLAARIAGHQRSFAIDLVVAVLVILV
                     LAYLSGATYVSVNDAVTRLSNGAESLANGDLSTRIGLESRDELGDVAERFNAMAAAFS
                     EVIRKVQQGADAVAGSSVSLAGSAARVSDGSEQQSAAASSMAAAVEEMTVGIEEIAVN
                     AASAEGVSSESGKLSQEGGAVVARTVSEMERIADAVQESARVIQELGDQSAQISTIVN
                     SIKEIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTAKATEEITTMVASI
                     QAGTGKAVDTMQDGVRRVQEGVQLAGQAGISMDQIRSGAGRVVTAVTDISAALREQGV
                     ASTEIARNVERIAQMAEQNSAAVRDTAGTARELERLAQQLRGDVQRFRI"
     gene            complement(22850..23938)
                     /locus_tag="PKX01_00580"
     CDS             complement(22850..23938)
                     /locus_tag="PKX01_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMZ74644.1"
                     /translation="MKLDEFVTAWQQPAPKRRIPMRQSRRSQLPRILLRIYFAAGVLL
                     GLIVLGTVGFYAAGGEQANLSDALYMTLITVTTVGYGEVVPIDGIAERLFAGLIALAG
                     FGAVTFLFTSLTVFFLESDLDYSLRRRRMEKQIRKLHGHFIICGYGRVGRNVGNEMTA
                     TNRHFVAIDCDEAGMESQRERSPGLVYLVGDASDDDLLMSAGIQDAQGVFAVTGDDSR
                     NLMIVFTAKQLNPSIRVVARCHEVRNAEKLRKAGADSVISPDFTGGMRIASSMVRPHV
                     VSFLDEMLRSEKKLRLEEVQVPPRFEPRDLGTLQLRSPNYVLLAVREGETFVFNPPKD
                     FRLQPGQHVIAMASPLGRQELETALLPH"
     gene            complement(23935..24468)
                     /locus_tag="PKX01_00585"
     CDS             complement(23935..24468)
                     /locus_tag="PKX01_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HMZ74645.1"
                     /translation="MRRLLKRHLPTPESLADHRWLAWWGAPLLHPRLWHLNRHSAAGA
                     VAVGLFCGLIPGPFQMLGAALCSVMFRVNLPLALFTTLYTNPFTIVPLYLAAFTLGQW
                     ILGPDGRTFIAPPDLDGSLLHWLGQLAHWAADLGRPLALGLVALAAVLACSGYVLTRL
                     AWRIHLVRAWRRRKGLA"
     gene            24592..26949
                     /locus_tag="PKX01_00590"
     CDS             24592..26949
                     /locus_tag="PKX01_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HMZ74646.1"
                     /translation="MSISGFLVGVVSCQFGAALPAPSCQFAAAVLAAGCGLAGALLYR
                     SSRERWPGLALVLSMLAGLAAGYAYAGWRAELRLAQSLPGDWEGRDVAVTGIVATLPQ
                     NFGRGVRFVFEVEAPAGGAVPRRISLVWYRGADDDEAGLLGEVHAGERWHLLVRLKRP
                     HGNVNRHGFDYEAWLLEQDLRATGYVRADASNRRLDAFVQSPGTLIERLREVVRSRFQ
                     AALPDGRYAGVLIALAIGDQRAIPQEQWSLFARTGIAHLVSISGLHVTMVAALCAAVI
                     GAIWRRVPALALRVPAQQAAVATGALAAGGYALLAGWGVPAQRTFAMLAWVALALLLR
                     REAAPFRVLGLALLGVLLLDPWAVLAPGFWLSFGAVALLFLAGSGRLAAGGRIREALR
                     AQWAVTVGMVPALLVLFQQCSLVSPLANGLAIPVVSFVVTPLALLFSVFPLQILADVA
                     HAVLGGVMLPVEWLGGLPYAQWQQAAPPFVLALMATLGCLWALLPVGTPARWLGASML
                     LPLLAWHPARPAPGALKMTVLDVGQGLAVHLQTAAHDLLYDAGPAFSADANSGERIVL
                     PYLRAQGVRRLDSVVVTHQDNDHAGGAEAVLGGIPVAEVRSSLPTAHPARLMAADRHL
                     PCRAGDAWEWDGVRFRVLHPGAEADADRPRKTNNLSCVIRVDAAGHSVLLTSDIEASD
                     EEALLARDPGALASDLVLAPHHGSRTSSTPAFVAATHPAWVLFPVGYRNRFHHPNPEV
                     WSRWSGAGARMARTDAEGALEVRVDAEGVQVEGARAVAPRYWYGR"
     gene            27062..28003
                     /locus_tag="PKX01_00595"
     CDS             27062..28003
                     /locus_tag="PKX01_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ74647.1"
                     /translation="MSLIDALRRLFTPAPVVVPPAAEVSAGGFEQPRFHRVQCAGPHG
                     LHRIAYTEWGDPANPRVLMCVHGLTRNGRDFDDLARSLAHHYRVVCPDVVGRGQSEWL
                     AVKDDYQLPTYVADMITLIARLNVETVDWVGTSMGGLIGMLVASLPEQPIRRLVLNDV
                     GPVITVSSLRRIGEYVGKAPVFPSYEAGEAFIRAVSEPFGRLSDAQWRQLAVHSLKQT
                     EAGWEMRYDPGIGEPFRKTPVLMDVELWSIYDAIACPTLVVRGAESDLLLRETAVEMG
                     RRGPKASLFEVPHVGHAPTLMEPAQIAPIRDFLLGSR"
     gene            28003..28689
                     /locus_tag="PKX01_00600"
     CDS             28003..28689
                     /locus_tag="PKX01_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HMZ74648.1"
                     /translation="MLATLVSAFSVVDWAALAWFFACWMGYGWVVEHGPKGGMGGLLG
                     ASHRFRLEWGRQMLRREVRVADSALVGNLMQNVSFYANTTIYVIAGLLALLGTVDKLI
                     DAAAELPFARHASREVWEVKVLLLLMVFVVAYFKFTWSLRQFNMLSILIGAAPPPGEG
                     SEADAQRFAKVNALAGDEFNRGIRAYYFGLAAVAWFVQPWLFMAVTTFIVLVLYRRDF
                     ASHTLAAMRE"
     gene            complement(28690..29088)
                     /gene="msrB"
                     /locus_tag="PKX01_00605"
     CDS             complement(28690..29088)
                     /gene="msrB"
                     /locus_tag="PKX01_00605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ74649.1"
                     /translation="MSRKIEKTDAEWRAQLNPEEYHVTRQKGTERAFTGRYWDCHDAG
                     EYRCVCCGAELFASEHKFDSGCGWPSFHSAAAPGNVEEAVDRSHFMVRTEVLCRQCGA
                     HLGHVFDDGPAPTGLRYCINSAAVKLKPKE"
     gene            complement(29157..30326)
                     /locus_tag="PKX01_00610"
     CDS             complement(29157..30326)
                     /locus_tag="PKX01_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HMZ74650.1"
                     /translation="MKRNRLGRRGGLPRDAEQLLWLASGLAESSSRAEDHFWDQRLAA
                     AVDGLLRAEDEDTLNTALDHLSSTNAAAYDELADMIESRAEGALTSDDPHDVLLIAAP
                     VMAWSRYRIPSATIPAAALANLRVQLKAHVLAKDVKLALADYLFSPDQLPQGYCATAD
                     FASHLARSAATDADLRIKTDNLPETAQFLSDTRYILGAVVVPKGAPIFRWQEEDCTRD
                     QAFEQWRAQGAACIAPLLTGCATEVVLPNAYFAASREADKASRPYSIQASVAFLSTTL
                     DAPATRLRAVVAPFFERQVEEFRIGFTLRNSNDVVHGVVWPLLGAEGETSETLGEIEA
                     TLRACGITDIQVLDNEFPMEYCDDCGAPMYPSPEGEAVHAELPEEQAEQMPKHLH"
     gene            complement(30403..31227)
                     /locus_tag="PKX01_00615"
     CDS             complement(30403..31227)
                     /locus_tag="PKX01_00615"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HMZ74651.1"
                     /translation="MSPTRPVFFISDGTGITAETLGHSLLAQFPDTRFRARRLPFIDS
                     LEKARDCALLIREAASETGLRPIVFNTLVDPAAVAALRESDALFLDLFEKFIGPLESE
                     LGQRSTHTVGRFHGIAESQDYARRIEAINFTLAHDDGVSHADLQEADVILVGVSRSGK
                     TPTSLYLAMQFGVKAANYPLIPEDFERNKLPGELARHRHKLFGLTIGPDRLSQIRQER
                     RPNSRYASIENCLGEIEAAQRMMKREGIRWLDSTAKSIEEISTIILQEVRLNPSSF"
     gene            31455..33824
                     /gene="ppsA"
                     /locus_tag="PKX01_00620"
     CDS             31455..33824
                     /gene="ppsA"
                     /locus_tag="PKX01_00620"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HMZ74652.1"
                     /translation="MVRKVIPFQELRMSDVEQVGGKNASLGEMISQLPASVRVPGGFA
                     TTAAAYREFLSHQGLADRISAALATLDVDDVVALAKAGAQIRQWIVDTPFPAALEAEI
                     KAAYEQLTTEGDGSFAVRSSATAEDLPDASFAGQQESFLNIHGYENILHAIKEVFASL
                     YNDRAIAYRVHKGFDHAGVALSAGVQRMVRSDIGASGVMFTMDTESGFNGVVFITASY
                     GLGETVVQGAVNPDEFYVHKATLALGKPAVIRRNLGSKLIKMVFADKKEAGKSTRTVD
                     VAEAERNVYSLTDEDVLELARYATIIEQHYGRPMDIEWGKSGDDGKLYILQARPETVK
                     SQQSGHVMEKYRLKQYGKSLTHGRAIGQKIGAGPVRVVKDASEMERVKAGDVLVTDMT
                     DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAIVGCGNATEVLDENDEVTVSCA
                     EGDTGYVYRGKLDFEVITTDMGNLPDIPVKIMMNVGNPELAFEFAQIPNGGVGLARLE
                     FVINNMIGIHPKAILEINQVPASLRNEINRRSRGYATPRQFFVEKLVEGVATIAAAFY
                     PKPVIVRLSDFKSNEYRKLLGGEIYEPEEENPMLGFRGASRYIAHSFRDCFELECAAM
                     KKVRNELGLTNVQLMVPFVRNLDEAAGVVDLLARHGLKQGENDLKLVMMCEIPSNALL
                     ADQFLQYFDGFSIGSNDLTQLTLGLDRDSGLVAHSFDERDPAVKQLLSMAIQSANKLG
                     KYVGICGQGPSDHADFAEWLMDEGIQTISLNPDTVVDTWLKLAAHSNKG"
     gene            complement(34030..35250)
                     /locus_tag="PKX01_00625"
     CDS             complement(34030..35250)
                     /locus_tag="PKX01_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ74653.1"
                     /translation="MKKSILAQSVALACLAAAAGGAQAVSFTQNDVTVDINGTINGFY
                     VNRESKVTNLSTGVQTKTQNSELTNGLLPGWINFVVTSKAGDQDIKAHFSFAPGINNN
                     STVVGLPIGNNASGAGVTNPYSQIDTRNVYFQFGNNEWGSLKFGRDIGLFGQNIILSD
                     MTLLGVGGTSNAGIPYNTTFGMIGHGYMYTGFQAQITYSTPNYNGLQASAGIFQPSKF
                     AGDETKAPGFQAKVDYDWKGAMPGKVWGGLVSQNTSCSNASTTACAGMKPYNATGYEA
                     GAKAGYNNFEAVAYGFTGKGLGLSTIGAQFLGGSDGNGNKTDSKGYFLQGTYKMDKTK
                     FGINYGQNKDTGGGTQSVYGAPETKNKAYTLGVYHGLNKFVTLVGEFNNEKITNTATE
                     TKNNTISAGAILFF"
     gene            35769..36587
                     /gene="pyrF"
                     /locus_tag="PKX01_00630"
     CDS             35769..36587
                     /gene="pyrF"
                     /locus_tag="PKX01_00630"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMZ74654.1"
                     /translation="MDFRSRLAAAWHSQDSLLCVGLDPDPARFPAHLKGRPDAVLRFC
                     LEIVDATADVACAFKPQIAYFAAARAEDQLEALIAHIHDRHPQVPVILDAKRGDIGAT
                     AQQYAREAFERFQADALTVNPYMGFDSVEPYLAYPDRGLIVLCRTSNPGGSDLQNLTL
                     SNGRLLYQHVAELVAEKWNLHGQCGLVVGATFPEELADVRQIVGDVPLLVPGIGAQGG
                     DVEATVTAGRTPLGAGLMISSSRAILYAGSGEDFAAAARQVALATRDDINRFRR"
     gene            37073..39046
                     /locus_tag="PKX01_00635"
     CDS             37073..39046
                     /locus_tag="PKX01_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HMZ74655.1"
                     /translation="MADKNRVGWYWKRLRDMSVSDIVGRAGSTAAQVVGRYRGAVPVP
                     PFRLDRTGARWLLPEVSAEGDFMGVADEVMSGHWRVLALDQAPLGLPPDWNCNPLDGE
                     HFPLNDGRTLSRREDGSGDIKYLWTLNRHRELVSLGLAWRKTGCRDYLDAIGVLMSDW
                     LEKSPYPHGPNWSSAVEVALRLMNWATVWQLIGGPNSALFAEPVGKVLRSDWLGSVWR
                     HAEFIQAHLSRPASAGSDRLGELAGLYVAGATWPYWSQAKVWRATGRDGLEQEVPRQI
                     SADGVVLEHSMAAQRMTWDVLSIAALAARATGREFSHALGCRLEAMAEYVAAVMDTGG
                     NVPQFGDSDDGDALGLGQAAGGCAYRSMLATGALLFSRPAFARKAGMLDPRTRWLMGV
                     DAARQFDLLLLKAFPSTLRTAFPEGGVYVLGAHLDTPLEWRIVADAGALGAGAMAEHG
                     HADALSFTLSVSGREFLVDPGSGTYHGQRAWRDAFRSTALHNTLVIGSEDQAVSGGRF
                     LWKRKYAVSVIRWESDADGDVLVAEHDGYRRLSGKPVHRRTWSFDRKTDSLSVRDEVI
                     ATMPQLVTLCWHFGEDCLVARSTKGVAVHNGPVRVDMVLPSGGDVSILQASEQPFAGW
                     ISRTYDRRVPATTVTWRGTLSPGDVLETEFRKG"
     gene            complement(39056..39907)
                     /locus_tag="PKX01_00640"
     CDS             complement(39056..39907)
                     /locus_tag="PKX01_00640"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ74656.1"
                     /translation="MITPQELVACIEKLTSLPAVYHRIRALLDDPDSSVHDLAEAVSS
                     DPAITARVLRVVNSVLFGFPGRIETVGRAVNLLGMQQVHDLVLGTAIVGAFNGIRPAR
                     MHMSRFWKDSVYRGLAARAAAHRLGLGDPERMFVEGLLADIGHLVMFQAAPDMADAAL
                     ERAHRHHIPIHEAEQSVVGCHYAEVGAALASAWHLPAGFSAAIGAQLMPLLAGPHTQE
                     AAILHLANDLLASDADEVDEDAVLATMSPSIVTILQADASMLGAVRARVRDELSAVIA
                     LFFPDQS"
     gene            40151..41035
                     /locus_tag="PKX01_00645"
     CDS             40151..41035
                     /locus_tag="PKX01_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ74657.1"
                     /translation="MHDRTEDPMKILSWNIQWGRGADGMVTLERTVSAVRTLGEFDVI
                     CLQEVAVAFPALQGGIPVDGVASIAAAFPDYSVHFASGVDMPDGNGGRSRFGNLTLSR
                     LPVGQVFRHSLPRPPEPGMPSMPRVCLETVVEGPQGWVRVMNTHLEYYSAVQRMTQIA
                     ALHTLQQDAVRLANAGGDTPGRNAAPGSPFEVLPRPASAVICGDFNCEPGSPEYFRMI
                     APIGPDVSGWEDAWRACYGDIPHLSTVGLNGAEWPDHAYCCDYFFVSADLVDRVAAVA
                     VDQLTAASDHQPIVLSLD"
     gene            complement(41171..41692)
                     /locus_tag="PKX01_00650"
     CDS             complement(41171..41692)
                     /locus_tag="PKX01_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ74658.1"
                     /translation="MPIYRLGERQPELGEGVWIAENATVIGSVVLGRNVNVWFGSILR
                     GDNDPITIGENTNIQDGSVLHTDDGVPLTIGRNVTVGHKVMLHGCTIGEGSLIGINAV
                     VLNRAVIGKHCLIGANSLIPEGKVIPDRSLVCGSPGRIIRELTDHEISRLQASAEGYV
                     KNAERYVQELNKL"
     gene            41925..42710
                     /locus_tag="PKX01_00655"
     CDS             41925..42710
                     /locus_tag="PKX01_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HMZ74659.1"
                     /translation="MSDFVLFERRDSIVTLTLNAPETRNALSSPAEWEALVDACERIQ
                     ADLSVKAVILTGAGSAFCAGGNVKDFRDKRGLARGGGLDIRENYRSGIQRIPLAFHRL
                     DVPTIAAVNGPAIGAGCDLACMADIRIASESASFAESFVRLGIIPGDGGAWLLQRVVG
                     YSKAAEMSFTGDRLDAAQALEAGLVSRVVAPDVLLDAAMALAERIAAQPGHALRMTKR
                     LMREAQTLRLESLLELSAAYQALAHGSADHEAAVAAFLDRPKG"
     gene            42718..43074
                     /locus_tag="PKX01_00660"
     CDS             42718..43074
                     /locus_tag="PKX01_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMZ74660.1"
                     /translation="MSDLDALQRRVKAFRDERDWGQFHALRHLIVSLNLEAGELLELI
                     QWRPDAEIDALPSDPVGREALADECADVLLYLLLIADRAGIDLGVAALAKLEKNAAKY
                     PVERFRGSRRKYNAPE"
     gene            43157..44002
                     /gene="rlmJ"
                     /locus_tag="PKX01_00665"
     CDS             43157..44002
                     /gene="rlmJ"
                     /locus_tag="PKX01_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HMZ74661.1"
                     /translation="MLSYRHAFHAGNHADVLKHFVLVQLLEYFNRKDKPYLYIDTHAG
                     AGCYGLEEAFARKNAEFEDGVARLLARKDVPAELKSYLRIVRDLNPPGRLRLYPGSPC
                     CAAPLLREQDRMRLFELHPADNRLLHQTFAEAGSKVMIKEEDGFTGINAFLPPPSRRA
                     LVLIDPPYEVKTDYRTVLTALKECLRRFATGTYVVWYPRLTTMHARELPEKLKRLPLD
                     SWLHVSLDVKKPSEDGFGMHGSGLFVINPPWTLPETLKTVLPWLKDVLAQDDGADFVL
                     ESQIP"
     gene            complement(44096..45391)
                     /locus_tag="PKX01_00670"
     CDS             complement(44096..45391)
                     /locus_tag="PKX01_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ74662.1"
                     /translation="MTVQEQYAAKRMSAAEAIRHVHNGDTIIVPTAVAEPPALLNALS
                     EHRRDFHDVKVSQILAVRKMGYLDPTTAQHVRHVAYFFSGATRPGFHEDVVDFRPSYF
                     SEMPQLIERGLMPAEVVFTQASPMDAHGYFSLGLAADYTMAAISKARAVILEVNENVP
                     YAFGNNQVHISQVTALVENNEPVLEVGLPAIGPVQEAIGKYVADMIEDGSTLQIGYGG
                     IPDAVVMQLTHKHDLGIHTEMIGDGILTLIESGAVTNRRKNYLPGKMVATFALGSKKL
                     YNWIDRNPAFEMHPVDFTNDPYLAGLNDNLVSINATLEIDFLGQCGSESLGPIPYSGT
                     GGQSDFVRAANRSKGGKSFIVLPSTAKNDTISRIVPTLTPGTHVSTSKNDINYVVTEF
                     GVAQLRGKTAKERTRALISIAHPKFRDELTETAKKMHLL"
     gene            complement(45530..46783)
                     /locus_tag="PKX01_00675"
     CDS             complement(45530..46783)
                     /locus_tag="PKX01_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HMZ74663.1"
                     /translation="MKQRRLLAAWTLAATLAGCATTGPLESRPSPTLPPAPGSPTPRA
                     PADIDRPRPVSPAPAPIGTAPTPPAPPPIPSAQDGRALIARFLPPGVSDREGWATDLH
                     TAFTALHLSLTPDTICAAVAVIEQESSFQADPAVPGLPKIVWRELEARRQRYHLPRLM
                     LDAALLAPSPDGRSYRARIDALRTEKQMSALFEEMISEFPQGKTLFADYNPVRTGGPM
                     QVSIAFAQDQIREKPYPYPRRGSVRDEVFARRGGVYFGVAILLDYPAPYSRLLYRFAD
                     FNAGRYSSRNAAFQNAVARLSGQALTPDGDLLRYRDGAPSSEPSATYSALSTLGGRLG
                     LSPAEIARDLRLEKTSQFAQTPLYIRIFTLADSLDGRRQPREILPEIDLKSPKIQRKL
                     TTAWFAERVDARHRTCLGRDRSFAP"
     gene            complement(46780..47022)
                     /locus_tag="PKX01_00680"
     CDS             complement(46780..47022)
                     /locus_tag="PKX01_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74664.1"
                     /translation="MAPPTLKQQKTFALIRIIGGMASAGVLGYSFVSNVLAGAPAEGA
                     LLATGLMAFLALAYAGYYTRVLGRVAQAEQQAADKP"
     gene            complement(47091..48098)
                     /locus_tag="PKX01_00685"
     CDS             complement(47091..48098)
                     /locus_tag="PKX01_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMZ74665.1"
                     /translation="MESLHISLWVVTGLVVVALLFDFMNGFHDAANAIATVVSTGVLK
                     PHQAVAWAAFFNFVALFVFHLKVASTVGKGIVDPAAVDHFLIFGALIGAIAWNVITWY
                     YGIPSSSSHALIGGLVGAAMAKAGTAPLISAGIWKTVAFILVSPLVGYLLGSLMLVIV
                     AWICRHKPQRRVDKWFRRLQLVSAALYSLGHGGNDAQKTVGIIWMLLIAAGVTAQGDP
                     VPMWVVVLCYLAIGLGTLFGGWRIVKTMGQRLTKLKPVGGFCAETGGALTLFMASSLG
                     IPVSTTHTITGAIVGVGSVRRTAAVRWGLAGTIVWAWVLTIPCSALIAALAYWAGLQI
                     M"
     gene            complement(48106..48732)
                     /locus_tag="PKX01_00690"
     CDS             complement(48106..48732)
                     /locus_tag="PKX01_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMZ74666.1"
                     /translation="MFGRLLPQEGQFFEYFNAHAAQIVLGAQELVALMEDLSSADRRI
                     SAIEGIEQSADRITHDTIAQLHKSFITPLDRDEIHSLINAMDDILDTVQDVAECTTLY
                     HLQRSTPEAVQLANISLSCCTRVKIIVGLISNMDNASAILQTCQEIDRLETDADRIMR
                     AAMSRLFRDERDALQVMKLKAIYEQLESITDRCEDVAKIVEGIVLENA"
     gene            complement(48939..50696)
                     /locus_tag="PKX01_00695"
     CDS             complement(48939..50696)
                     /locus_tag="PKX01_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ74667.1"
                     /translation="MTDAIERFDQLDLQPAILAALAEIGYETPSPIQAACIPHLLAGQ
                     DILGEAQTGTGKTAAFALPALDRVDLARREPQVLVLTPTRELAIQVAEAFQRYASRMA
                     GFHVLPLYGGQSMVVQLRQLQRGAHVIVGTPGRIMDHIERKSLNLDGLATLILDEADE
                     MLRMGFIDDVEWILEHTPAERQTALFSATMPDAIRRVAHRYLREPREVKIKAATATVS
                     TIHQRYLQIAGAHKLDALTRILEVEDDFDAAIVFVRTKTATVELAEKLEARGYAAACL
                     NGDMTQQLRERVIEQLKAETLDIVVATDVAARGIDVPRISHVINYDIPYDTEAYVHRI
                     GRTGRAGRTGNAILFVAPRENHLLRQIERATRQPIEALKLPSREAVADKRVAAFRQQV
                     AEVLASEELDFFLDVIAGIEDAHDAEIHEIAAALAFLAQKERPLQMPDNGQPDIAQFA
                     GSAQRERPARENRGEILERRRDYAEGRLMRYRIDVGREHLASPRDIVGAIANEGGIDS
                     RYIGQINLYEDYSTVELPANLPPDVVQTLRRARVRQQALNLRPASAEESARPRGGRPA
                     GKPPRKDGPGGFKPRKPRA"
     gene            complement(50808..51512)
                     /locus_tag="PKX01_00700"
     CDS             complement(50808..51512)
                     /locus_tag="PKX01_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HMZ74668.1"
                     /translation="MIDLHYWTTPNGHKITLFLEETGLPYRIVPVNIGRGEQFQPDFL
                     RIAPNNRIPAIVDHAPADGGAPVAVFESGAILLYLAEKTGRFLSTELRVRLDTLQWLF
                     WQMGGLGPMAGQNHHFVQYAPERIPYAIERYVKETARLYAVLDRRLADRDFIAGADYS
                     IADMACYPWIVPHDRQQQDLAHFPHLARWFDAIRARPATVRAYALAERIHAAPVVDDA
                     ARQVLFGQDAGTVKRP"
     gene            complement(51524..51880)
                     /locus_tag="PKX01_00705"
     CDS             complement(51524..51880)
                     /locus_tag="PKX01_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HMZ74669.1"
                     /translation="MCQIFINADPDLYATRSRSLRLHGVATSLRLENLFWQVLEEIGA
                     RDGLTVPQLTSRLYDELLQSGRLDDSTNFASFLRVCCLRYLRLQLSGRIPADTAVPIR
                     ELDPAWVLTAERHPCA"
     gene            complement(51952..52530)
                     /locus_tag="PKX01_00710"
     CDS             complement(51952..52530)
                     /locus_tag="PKX01_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HMZ74670.1"
                     /translation="MTKKILMICGDFCEDYETMVPFQALLAVGHRVDAACPGKKAGDT
                     IATAIHDFEGDQTYTEKRGHAFALNATFAELRPEDYDALLIPGGRGPEYLRTYPTVIA
                     AVRHFFEAGKPVAAICHGAQLLAGARVLEGRTCSAYPACRAEVELAGGTYADIPIDQA
                     VTDGNLVTAPAWPAHPAWIAQFLAVLGTRISL"
     gene            52681..53376
                     /locus_tag="PKX01_00715"
     CDS             52681..53376
                     /locus_tag="PKX01_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ74671.1"
                     /translation="MAYVIEPPRPVVVPVLGDGLFPVRRVYCVGRNYAAHAREMGADP
                     TREAPFFFCKPADAVVSVGPEGMLELPYPVATANLHYEAELVVAIGLGGADIPVDEAL
                     AHVWGYGVGLDMTRRDLQTALKDKGRPWELGKAFDRSAPLSTLVPAGAIGHPQRAAIW
                     LERNGESRQRADIADMIWSVAEIVAHLSRYFRLEPGDLVFTGTPDGVGPVVAGDVVEA
                     GVEGVGRLRVRYV"
     gene            complement(53393..53836)
                     /gene="queD"
                     /locus_tag="PKX01_00720"
     CDS             complement(53393..53836)
                     /gene="queD"
                     /locus_tag="PKX01_00720"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HMZ74672.1"
                     /translation="MRITRRLEFDAGHRIPDHASQCRHLHGHRYALEITLSGDVVRAD
                     GAPINGMVMDFADVKAIAKEHVVDVWDHAFLAYRHDTAVVGFLQSMPGHKTVILDVVP
                     TAENLAAEAFRILDPLYRDVYGNHLRLERIRLYETPNCWADALRD"
     gene            53902..54753
                     /locus_tag="PKX01_00725"
     CDS             53902..54753
                     /locus_tag="PKX01_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ74673.1"
                     /translation="MTASSQMLTVADHNRSFSALTYVYPVVSRRARGVSVGINLNPNN
                     ACNWHCAYCQVPGLVRGAAPSIDIELLAAELQGFLTELQQGQFMKDHVPEEMRVIRDI
                     AFSGNGEPTSCKAFADIMERVVGIRNACGLRNVPIRVITNGSLADRPSVQAAFRTLAE
                     AGGEVWFKVDAGTREEILKINGVDIDPAAHARRLALTASICPTWVQTCVFRWDGELPT
                     QEFSDSYVAFLQKAGLEGLKGILLYGVVRQSHQPEAIHLQPLSAEELEHIAVALRKTG
                     LTVNVSP"
     gene            complement(54767..55069)
                     /locus_tag="PKX01_00730"
     CDS             complement(54767..55069)
                     /locus_tag="PKX01_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ74674.1"
                     /translation="MKTNEKVDVREIRRKLGMNQSQFWSKIGVTQSGGSRYESGRNIP
                     RPVQALLRLVHIEQVDINKIKKDDVEVVEYLKATNPELFKTLKKEARAKRKERVPH"
     gene            55377..56606
                     /locus_tag="PKX01_00735"
     CDS             55377..56606
                     /locus_tag="PKX01_00735"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ74675.1"
                     /translation="MALIVQKYGGTSVGNPERIKNVAKRVAKFHAQGHQTVVVLSAMS
                     GETNRLIALAKEISGSPSPRELDVVVSTGEQVTIGLLCMALEEVGVPARSYTGGQVRI
                     LTDSSFTKARILDIDGDNMRRDLDAGRVVVVAGFQGVDAEGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFQEEGEGTLITVEEDKNMEQPIISGIAFNRDEAKLTVLGVPDKPG
                     IAYQILGPVADANVDVDMIIQNVGHDGTTDFSFTVNRSDYDKTVKILQDVQAHVGARE
                     IKGDRTMAKVSIVGVGMRSHPGVASRMFRTLAEEGINIQMISTSEIKISVAIDEKYLE
                     LAVRVLHRAFGLDQAAA"
     gene            56650..56743
                     /locus_tag="PKX01_00740"
     tRNA            56650..56743
                     /locus_tag="PKX01_00740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56684..56686,aa:Ser,seq:gct)
     gene            complement(57266..57919)
                     /gene="narL"
                     /locus_tag="PKX01_00745"
     CDS             complement(57266..57919)
                     /gene="narL"
                     /locus_tag="PKX01_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HMZ74676.1"
                     /translation="MTATEAPSTVIVIDDHPLFRKGVCQLIALSDRLRLVGEASSGED
                     GLTLAQTADPDLILLDLNMKGMSGIDTLRALRDADLGARIIILTVSDAAEDLVAAIRA
                     GADGYLLKDTEPEDLLARMSEALAGRIVIDDSLTGLLARSLREEAQSAERSLAHLTER
                     ERAILQCLATGLSNKLIGRELGIAEGTVKVHIKSLLRKLKFRSRLEAAVWAIEQDTR"
     gene            complement(57916..59799)
                     /locus_tag="PKX01_00750"
     CDS             complement(57916..59799)
                     /locus_tag="PKX01_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ74677.1"
                     /translation="MRPPDAAAFVTARAPRRLSVVARLGALIAAIGALAVVSILASMT
                     VAELASGQARAINLAGSLRMQSYVIAATLADGEHGALLERALSGFDERYAAQALTTAL
                     PASAEASIRLAYDDVGLFWAQTFRPYAEAAPEPDRDARNRLTADLVARIDHLVALIEH
                     NLESRLQTLRLVQGISLFLLLVLGGVVVHQINRQVRAPLGELLTCARVVRRGDFSVRA
                     APRPPDELGQLGEAFNYMVEDLSRSYSQLETRVEEKTRELARSNQSLELLYRTTRALS
                     EADLTQDSLLKVLNEVERLAGVRAGALCADPGDGRPLVVLATDMDTDLVARLPLDGPP
                     SAAPAALQVLPALSYAGDGALITVPVSDGVRSLGRLFLQLPDSAPPAEWQTPLLETVG
                     RHVGAALANARRHEERHRLALLDERAVIARELHDSLAQSLSYLKIQVTRLQNLLARQS
                     VSDPVGDVVRELRDGLNEAYRQLRELLTTFRLRIDGRGLTAAIDDSVREFSQRGGLAI
                     HLSNRIAGLELPAGREIHVLQIIREALSNIERHARAHTVDIVLERLDAGDLRVCIDDD
                     GVGIGEQPAPPRRFGIAIMHDRARSLDGALTVTARTDRGTRVELCFPAEPPAPPSPRG
                     ISP"
     gene            60117..60875
                     /locus_tag="PKX01_00755"
     CDS             60117..60875
                     /locus_tag="PKX01_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ74678.1"
                     /translation="MKTSPVELSAVLPGIRLFAGLDPRAVAELSRAGHVATLPKGRGI
                     YATGELPRALYYLLSGQVKVAVTSAEGGEKVIDIVHAPQAFGVAELFGQAPHVSSAET
                     VGASVVLVIGREGVMRAIEYDARLSMRLLQTVADRQTAIERDIAADCFQSGCEKVVDY
                     LLRAVAPAAGWGDSVSLDLEIPKYLLAARLGFTPETLSRIFRELSDEGLIRVHGRRVE
                     LLGRLLRRHGVVPAQVRPPRSPERGRSGQAVAWA"
     gene            complement(60854..61081)
                     /locus_tag="PKX01_00760"
     CDS             complement(60854..61081)
                     /locus_tag="PKX01_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HMZ74679.1"
                     /translation="MNEAKLVNMANQIATAVAANAEPARAAIDVAEHLMRFWDPRMRA
                     GLIACAGGAAGLDPLVREAVARLRDQAQATA"
     gene            complement(61074..63929)
                     /gene="fdhF"
                     /locus_tag="PKX01_00765"
     CDS             complement(61074..63929)
                     /gene="fdhF"
                     /locus_tag="PKX01_00765"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HMZ74680.1"
                     /translation="MSLPCPPDAGTPARDGEATVRLLIDGQPVCVPAGTSVMHAAHTA
                     GCAIPKLCATDRLEAFGACRLCLVEIDGRKGYPASCTTPVAPDMVVRTHTPRLAELRR
                     NVMELTISDHPLDCLTCAANGRCELQDMAGAVGLRAVRYGLDGANHLGDRADDSNPYF
                     RYDPSKCIVCNRCVRACEEIQGSFALSIVGRGFDSRVRAGQDESFMASECVSCGACVS
                     VCPTATLMEKSVIEHGQGERRVTTTCAYCGVGCSFDAEVKGNSVVRMEPTRDGGANQG
                     HACVKGRFAWGYATHPDRVTTPMIRPSIDAPWQKVSWEEAVTHAAAEFRRIQAEHGRG
                     AIGGITSSRCTNEETYLVQKLVRTAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQD
                     FASVLKADVVVVIGANPTDGHPVFGALLKRRLRQGARLIVVDPRAIDLVRSPHVEATH
                     HLQLLPGTNVAMINALAHTVVTEGLADEAYVQARCDLASWQAWRDFVARPEHAPEAVE
                     AVTGVPAEAIRAAARLYATAGNGAIYYGLGVTEHSQGSTMVMGIANLAMATGNVGRPG
                     VGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDATVRGSFEAAWGVRLDPEPGLRIPN
                     MLEAALDGSFRGIYIQGEDIVQSDPNTQHVEAALAQMECVVVQDLFLNETAKFAHVFL
                     PGASFLEKDGTFTNAERRISRVRRVMPPLSGKADWEATLALAAALGHPMPYDHPSDIM
                     DEIARLTPSFSGVTYELLERRGSVQWPCNADAPEGTPTMHVDRFIRGQGRFMLTPYVP
                     TSERVTRRFPLLLTTGRILSQYNVGAQTRRTPNVAWHGEDRLEIHPTDAEDRGIRDGD
                     WVGIASRAGETVLRATLTERVQPGVVYTTFHFPESGANVITTDNSDWATNCPEYKVTA
                     VQVTLCAQPSDWQQRFALRHHRQRGLQATATGGIPHE"
     gene            complement(63942..65489)
                     /locus_tag="PKX01_00770"
     CDS             complement(63942..65489)
                     /locus_tag="PKX01_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ74681.1"
                     /translation="MNVRLFVPADSGALSVGADDVARALVREAASRGVTVELVRNGSR
                     GLYWLEPLVEVDTPAGRLAYGPVSVEDVPGLFDAGFLTGGMHRLGHGLTEHIPFLAGQ
                     ERLTFRRVGVIDPRSLADYQNHGGFDGLRRALALGDAAVVREVTDSGLRGRGGAAFPT
                     GIKWATVQTAGDGDKYVVCNADEGDSGTFSDRMLMEGDPFCLVEGMTIAGLAVGARRG
                     YIYLRAEYPHAFRVLEAALATARSAGWLGDDVAGSGQAFDIELRLGAGAYICGEETAL
                     LESLEGRRGIVRAKPPLPALSGLFGRPTVINNVMSFAAVPLILARGAAFYRDYGMGRS
                     HGTLPFQLAGNLRHPGLVEKAFGITLRELLLDYGGGSASGRPIRAVQVGGPLGAYIPE
                     SRFDVPLDYETYAAMGAMIGHGGIVAFDDRADLGRLARYALEFCALESCGKCTPCRIG
                     SVRGVELIDRLLAGTDRPRDRALLDSLCDTMIHGSQCALGGMAPLPVQSLLTHFPDAF
                     GLAPTAA"
     gene            complement(65486..65965)
                     /locus_tag="PKX01_00775"
     CDS             complement(65486..65965)
                     /locus_tag="PKX01_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HMZ74682.1"
                     /translation="MNDVLHAAVTDALARHGHRPDALLPLFHALQDRIGWVPPDAVPM
                     LATALNLSRAEVHGVLSFYPHFRRTRPGRHVLQICRAESCQARGGEALEAHARSLLGT
                     GWHGTSADGAVTLEPVFCLGNCACSPAVRIGDELLGRMDVARLEALIARLRTSEPAP"
     gene            66155..67057
                     /locus_tag="PKX01_00780"
     CDS             66155..67057
                     /locus_tag="PKX01_00780"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMZ74683.1"
                     /translation="MLGAADSARDEVHSSAMRRWGRIFGVVAGGALGVLAVPVIVSQT
                     SLSGEALTETARGVGWGALVLAAGLAGRWAQGRRAHRVAQTEGAAAVATAPSGSQPRR
                     ARAWSVDVLQQLDRARFQALCCAFFRESGVAATTHAGDEDSSAGDIRLHQDPRRPGAV
                     TAVVRCVPWGPTIDVWAVRALAETRRRESADRAFFMAPGGFAPDALAFAAQGRMTLFD
                     SRLMLALIRRLPEDSQQRLLDLAATGDWTAPACPECGERMVVRNVIRRPFWGCSRFPQ
                     CRGTLPMNRKAGLQGDDGPLAMAG"
     gene            complement(67150..68124)
                     /locus_tag="PKX01_00785"
     CDS             complement(67150..68124)
                     /locus_tag="PKX01_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HMZ74684.1"
                     /translation="MRAYLCQAFGPLDQHGPGQIGDPQPADTEIVIDVAAAGVNYYDT
                     LIVQGRYQIRPPLPFSPGGEFAGTVCAVGAQVHEFTVGQRVLAFNSFGGYAEKARARA
                     AQVFPLPDAVSFESAAAALVTYATAWFALHDRGRMQPGETVLVLGAAGGVGLAAIQIA
                     RAAGCTVIAAASSREKLDLCAAAGAHTGIDYHANDLKDAVKAATGGRGADIVVDVVGD
                     RYTEAAVRATAWRGRVLIIGFAAGEIPRVPANLLLLKGCEMAGVFWDEFLRREPAECR
                     RQVAAILDGIARGIFQPPIHGRYGIEEAGQALTLLADRQATGKLLIVP"
     gene            complement(68154..68933)
                     /locus_tag="PKX01_00790"
     CDS             complement(68154..68933)
                     /locus_tag="PKX01_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ74685.1"
                     /translation="MHTLALRGHTLEVVRRPSAHPRPEAPALVFLHEGLGSIAMWRDF
                     PQRVADATGCEAIVYSRHGYGRSSPLTAPRTPDYMHVEALQILPAVLDALQLRAPILV
                     GHSDGGSIALIHAGGTPAPVRGVVVMAPHLYVEDVALEGIRRTVDAYRTTDLRARLAR
                     FHADVDGVFAGWSDIWLHPDFRDWNIEAFVSAIPCPVMAIQGEDDEYATMDQIDRIAR
                     LAPDVDLVKLADCRHSPHKDQADAVVEAIAGFVARLLDDPH"
     gene            69101..69177
                     /locus_tag="PKX01_00795"
     tRNA            69101..69177
                     /locus_tag="PKX01_00795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69135..69137,aa:Arg,seq:acg)
     gene            69259..69335
                     /locus_tag="PKX01_00800"
     tRNA            69259..69335
                     /locus_tag="PKX01_00800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69293..69295,aa:Arg,seq:acg)
     gene            complement(69453..>70374)
                     /locus_tag="PKX01_00805"
     CDS             complement(69453..>70374)
                     /locus_tag="PKX01_00805"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HMZ74686.1"
                     /translation="RMGASRREGKQLLGFFDDRHAERLASPRLRLLGRIADLADYVRQ
                     QGVDVIYIALPMTTQPRIVRLLDDLRDTTVSVYFVPDLFVTDLIQARVDHVGGMPVVA
                     VCETPFTGLNGVLKQSFDFLMAACILILIAPLMLVIAAAVKCSSPGPALFRQYRYGLD
                     GERILVYKFRSMTVCENGETIAQARQNDARVTPLGAFLRKTSLDELPQFINVLQGRMS
                     IVGPRPHAVAHNEMYRKLIKGYMIRHKVKPGITGWAQVNGCRGETETLEKMKQRVDFD
                     LEYLRRWSLALDLRIILRTVRLIRKDPNAY"
     assembly_gap    70375..70474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            70609..71949
                     /locus_tag="PKX01_00810"
     CDS             70609..71949
                     /locus_tag="PKX01_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74687.1"
                     /translation="MLGVGSPVLALDASALRVSTLLGEASRQAAPLFESDELDPPALT
                     LTGTLGPEAAGLPERGGEPLDLPVGKSAGTRISVAENLTYDSNVFRLRDSAAAVAAGL
                     AHLGSWQSQTKLGLGLDKSYAGQQLLLDYDATMARYSHFGFLDHTVQGGIGRLRWKAG
                     QDLKGELVSEYREALDDYAYYRSPLRSISTTRQMGGNAFYTLVGGWQVGAQAGRGSRR
                     YKDGTRPTNEIDFAGVDGVLRYVPERGGAVALTRRRARGEYPNLPATAGSLAETRFNQ
                     DETFLEVSLPVRSGSRYLMRVGRVSRNYERYPQSNFSGRNGLIGAEWQVTPRTQLNGS
                     LRYDVEPAQDFVSSYVATKGARLGAVWEYSPKTRIESRYEWRNAQYRGDPGFGVVAAS
                     RREDTATLVGVTALHAMSARTRWTASLSRERRDSNQQEWSFTDWIVAGSLQWVF"
     gene            71946..72782
                     /gene="epsE"
                     /locus_tag="PKX01_00815"
     CDS             71946..72782
                     /gene="epsE"
                     /locus_tag="PKX01_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HMZ74688.1"
                     /translation="MKQGREGWQRWLLCVVAMLAAALTGSLALAQGPAPAAQEYRLGP
                     GDGVKISVFQNPDLGLETRVSETGVISYPLVGSVQVGGLTVGEAEKAIAAGLRSGGYV
                     LQPQVTLVVQQVRGAQVAVLGQVARPGRYPLESTAMKVTDALALAGGTTPQGAEKVIL
                     TGLRNGRRVRLEVDVADLFVTWTPEKDVPVHAGDTLYVQRAPQFYVYGEVLKAGAYRV
                     ERNMTVLQALSLGGGLNPRGTERGIRIHRRGDDGSVRVINPQLNDPVQADDVIVVREA
                     LF"
     gene            72796..74175
                     /gene="epsF"
                     /locus_tag="PKX01_00820"
     CDS             72796..74175
                     /gene="epsF"
                     /locus_tag="PKX01_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HMZ74689.1"
                     /translation="MGITQLLAVLKARRTLLFGVWGGVVLLALLVSLILPRQYAATAE
                     VVVESRGSDPLAPPQGVGLTTPGFLVTQADIIASERVGARAVAALKLMDQPRWAERWQ
                     KASGGQGDPARWIAGKLKKTVKVSPSHESNVLTITVTADDPAFAASYANALAKAYLET
                     DLELKIEPAREYATWFDEHTKVLRANLEQAQSRLSAFQREHGLVGDEKLDLESARLGE
                     LSTQFTAVQAQSTEATERQRQGGGANEVASNPLIQALKVDLARQEAKLTELSGYLGEN
                     HPQYQRTKSEVESLRARLRQETGNVAAMVGTNSRISQAREGDLKGRLDAQKQRVLALK
                     QQRDEMMLLQREVDSAQKAYDLVMARLTQTSLESKARQGNVALLSSAVAPTEASSPKV
                     LLNVGLALVIGALLGLAVTIVRELFDRRVRAAGDLADALGVPLVVEVKAAPAAAAGAR
                     PFWPRLRRA"
     gene            74185..75075
                     /locus_tag="PKX01_00825"
     CDS             74185..75075
                     /locus_tag="PKX01_00825"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HMZ74690.1"
                     /translation="MDKAKFSGFGNVAPLERARDATPSLGELLVSAGRLSADAASRVA
                     TRQRERGIPFGQAAVELGLVSEADVRAALARQFDYPVLAAGDPSVAPELVAAFTPDAP
                     QVEALRKLRSQLVLRRAGNPHCKTVAVVGAGRGEGRSWLVANLAITFSQLGERTLVMD
                     ADLRTPRQQALFRLPPAGGLAAHLAERGDSQPPVVHPRFPGLAIQPAGNVPPNPQELL
                     ARSGFAAHVTAARDFYDMVLVDTPADEVGADAQLVAAACGFAVVVARRHITAHKRAGA
                     LAASLRDAGVTILGGVLLEF"
     gene            75075..76310
                     /locus_tag="PKX01_00830"
     CDS             75075..76310
                     /locus_tag="PKX01_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74691.1"
                     /translation="MRFALPAFDVAELTAFHLENRVARALTWAMFVGLWAKLAVSVLL
                     VQPVIDAEADSFYSRAVDAMLLCAIFVGLATYADELLRRAPLVLAGVPLVLASFMTTE
                     VLTLDGFALTTFEYLKIVTPLLMSAVLLRLFEREPALVVRLMQLTLAIVWLGVIGALL
                     FLPPSLNRGGEPFWPVYFASLHTSSYLVVVCAGLSLYLYEADHFGKRLFIAQILFSFV
                     LVFHGWGVRTSMVAAVAFGLVYFFQHSRKAALVVFLTIVAVLTLAYLPLLFGVVHLPS
                     WDEMVELSSGRMSMWLQKADILANSTLLEWLFGHGEGSDWIVSEIWWWSAKDSHNDLI
                     RLLNQQGLLGLGGVLWLLWVWARLFPAGVAAPLLAALLASTAFSNGIMFRPQAGLLMP
                     LALVAAADAWQRRQAGRAA"
     gene            76307..77548
                     /locus_tag="PKX01_00835"
     CDS             76307..77548
                     /locus_tag="PKX01_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74692.1"
                     /translation="MSRDLTALVRRVEVLAVTAAPGGQRLLHFMLVQMACGTEALGRF
                     ANDLAIAMMLGLFSAVGWSALVLARVPVAPAGRSAPVMLGLARGVAVFTALACLPLPL
                     LAWGGWVFEPGWTALLLAGWTAYTVARHYGLALGAYRVLLVVETLVVVLLAAGLLIFG
                     RAHPVMAWAAFAVPTVLLGALAVLVLIRRARDQGGAAPLPAGSARTAAEQAALNFVSG
                     GMSMILVPCTVQVAGEAYGGLVALIGSVTSVLLLFPRALSFNALPALARVVRQQGAAD
                     VRALLDALRRTLFRVNSGLTLVAVAAWGLMAGVGERPAFALAGATAIFLLQLASFYVG
                     QLVLPEANYLQTVEETRLPLVVNLASLGLFVVACGGVMLLVPKGVDAVLGLFAVLLGA
                     TFARNLLLRRAVLHRAPEVFA"
     gene            77545..>78172
                     /locus_tag="PKX01_00840"
     CDS             77545..>78172
                     /locus_tag="PKX01_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74693.1"
                     /translation="MNVLRLSIFLPDGPVPGWVVRLLDVCGAEPGVILVGVLYDAQPM
                     AAADWAEQVDRLLFDRGASSLDPVSLELPGLLPVWRAEAGVAPARWLAERACDVLLDL
                     RPRPTPIPQLAAEHWMLSIAGRPATDAGAGAQPVVGGDATTCVALERVGGGAILDTSV
                     GATCEFSIRRNRRRALAKGIGMVRRALRRMRTQVPPPPQRDTLPAAAVQ"
BASE COUNT        12549 a        23487 c        28132 g        13904 t
ORIGIN      
        1 cggcggggct cgaaggcgcg gtgcagcttg gcgaccagtt cgagggcttc cggggtgagg
       61 atggtttcgt agcccgggcg gatcggggca ttgatctgca tgccggcggg gaggctcagt
      121 tggatgctca tggtgactcc tgatgaaaaa acggtgaggg attgaaaggt gtttcggtcc
      181 gggcggctca ggcccggtgg cgggcgacgt aatcgaccac gtcgctgagc tggcggcccc
      241 gggcgacggg actgacgtcg agctgctcct ggggcaggcc gctgcggttg atccagaagg
      301 tggtgtagcc ataccaggtg gcgccggccg cgtcccagcc attggacgag acgaacagga
      361 tacgctccgc cgggcagccg aaggcggcgg ggccgagggc ataggcgtcg gcgctggtct
      421 tgtactgctg gacggcgtcg acggacagca cgtggtcgaa gaggccgttc atgccggcgc
      481 tcttgatggc cacgtcgagc atctcgggcg tgccgttgga caggacggcg gtgggcacgc
      541 cggcttcctt gagggccttg agggcgccga gattctccgg aaagggcgac aggcaggcgt
      601 actggttcat cagctgggtg cgccgcgggg cgtccagttc caggccgagg cgggccgccg
      661 catacacgag gccgtcttcg gtgacggacc agaagggctt gtagcggtcg gagagggtgc
      721 ggatgaagct gtattcgatc tgcttctggc gccacaggtc ggccagctgg gcgcccctgc
      781 cggggtagag ctgctcggcg agcaggccga cggagtacac gtcgaagagc gtgccaaagg
      841 cgtcgaaggc aactgcctgg atccggggag cgtgggccat ggattgttcc ggtgaaacga
      901 tgtcgggttg ggcccgcggg gacgcttctg ggccggttcg tggccggctg gcatcctccg
      961 gggcggtgtc tcctcaccct gaatggcgtg catcttattc aagcatcaaa caaaaaagaa
     1021 gccataatgc gcgcaaatga ttttttactt ggagtatcga atgagcaact tcaaggagat
     1081 cgaagccttc gtcagcgtgg ctgcgcgggg cagcttgtcg gccgcggccc gggcggacgg
     1141 ggtgacgccg gcgatgatgg ggcggcgcat cgacgcgctg gaggaacgtc tgggggtgaa
     1201 gctgctggtg cgcaccaccc gcaagctgag cctgactttc gaaggcagcg cgtttctgga
     1261 ggactgccag cgcattctca acgatctggc caacgcggag gcgtcggtga gcctgggcgg
     1321 ggtgaaggcc agcgggcata tcaaggtgtc ggcgccagcg ggcttcgggc ggcgacatgt
     1381 ggcgccgctg gtgagggatt tcctggcggc ccacggggac gtgagctgca gcctggacct
     1441 gagcgaccgc ctggtggacc tggtgaacga gggcgtcgat tgcgcgatcc gcatcgggga
     1501 tctgagcgat tcgagcctgg tgtcggtgcg tctggcggac aaccgccggg tggtggtggc
     1561 aagcccggcc tatctggcgc tgcacggcgt gccggccacg ccggaggatc tgctgagtca
     1621 cgactgtctg tcgctcaatc cgcagcgcgg ctgggtgttc tgcgatccgg acgcgccggg
     1681 cacgacgcgg gtggtgaagg tgtccgggcg cttcgaatgc aacgacggta cggtgctcca
     1741 cgagtgggcg ctggcgggca tcggcctggc ctggcgttcc atgtgggagg tgcagcggga
     1801 tctggcgcag ggcgcgctgg tgtcggtact ggatgccttt gcggcgccga gcaccggtat
     1861 ctacgcggtc tttccgcaga accggcgcct gccgctgcgg gtgcgcctgc tcatcgacca
     1921 cctgcggggc agctttggtc agccagacta ctggcgggcg ggctgagtcg gtgacgattc
     1981 ggtgggtgtt tctggcggcg gcgggttgag ttcgaactcg atgcgcaggc gaaccggcac
     2041 cgggtgcttg cggtattcac cgggggtgaa ttcccaggtg cgcacgatgg ctgcgtaggc
     2101 gtccgccgcg gcacacaggg cttcgtcgca ctgggtgttg accgccgcca ccttgccgtc
     2161 ggccgccacg atgacctcca gctggaaaat gcgatggccg agttccggga aggggggcca
     2221 gatggaggcg ggcaccggcg tgagcagatc gggcggcgag ctgagggcgg agaggggcag
     2281 ggcgtccgcc gaagggggcg tgtcgtccgc gcctggcgtg gggggctgct cgacgggcgg
     2341 gggctcgggc gggtcggccg gggcgggcgc aagagagagc gcgtcatccc ttcgttcagc
     2401 gatggccggc gtgaatgtga cctggaccac ggcaggcgtt cgccccgccg ggccggggcc
     2461 tccaaaatac gtcgatgccg ccgttatcac ggctacgtgg agcacaaccg acaggctgcc
     2521 ggccaggcag aggctccggg gaaacgacgt gtgcatggct gaacggggcg gggcaaggct
     2581 gttacccggt aggcgtgaac gatagaggca ctatgtcacg gcgctgttgc cgcctgtcgg
     2641 tgaccggaat ccgtcggtcg cattggactc gcgtttgaaa tctgtcggtg tcatctttcg
     2701 ctcgaaggag agcgtgcaat gaagcgtcaa tcgggtgtga ccctgatcga gttgttgatc
     2761 gtcgtggtga tcgtcgccat tctgggcggc atcgccgtgc cggcctacac ggaccacata
     2821 ctgcggggca agatcgcgca ggtcaccgag aatctggccg aggccaaggt gaggatggag
     2881 caggtcttca attccaaccg gacctacgcg acttcgccgg gcgggacgag ctgcccggat
     2941 ttcagcaccc tgttcgacaa gaccggattt accctgaaga tgagcgattg tccgggagat
     3001 gcgggaacga ccttcaccct gacggcgacg ggcagttcga gcagcggtac gggtggattc
     3061 atgtattcca tcaaccagaa cggcgagaag aagtcgacga cgccggccac ctccgaggtg
     3121 caatcctgct ggttgatgtc caagacggcg tcttcatgtt gattccggcc cggcgcctgc
     3181 ggggcgccac ccttctcgag ctgatggtca cggtgtccat cgtggccatc ctgatggcag
     3241 tgggtatgcc gtcgatgaag gactggattc tccgcaactc ggtcagcagt gccgccgaag
     3301 ttttgcagaa cggtctgcgc cgcgccgaga gcgaggcgat ccggcgcaat gcgcccatcg
     3361 agttcctgct caccagcgga attccctcgt cgtcggcatc cctgacggcc gaggccaacg
     3421 cggtcaactg ggcgatccgt gtggtggaca gcagcaacag ctacaagccg gtggcgggcg
     3481 ggtatgtggc aggtttctat ctgaaggaca tctcgtcggc ggtaacggtc agtggcccgg
     3541 ccagcgtgat cttcaacggc atggcacggg ttctggatag cgcgggagtg ccggtgaacg
     3601 ggtatcaggt gtatcgcttc tcgcgaagcg gctccgacaa ggcttactgc gtctttgtgt
     3661 cccagggagg aggcgtcaag atgtgcgatc cggatcaggc gtcgggcacg ccgcgggcgt
     3721 gccagcctct gttgagcgca tcccagtgcc cgaaggcggg gagtggatcg tgaagcgtcg
     3781 gtcctttgca ggctatgtgt tgctggaggc cctggtgggg ctgctgatct tttcgctggg
     3841 tctgctcggc ctgatcggat ttcaggcttc agccatgaag atcgcgtcag acagccgttt
     3901 ccggacggag gcggcgatgc tggcagatga actgattggc aagatgtcca tgtcgaagat
     3961 ttcgaccgtg cagaccgaat attccgcgga cggctccaag ttttccgact ggctcaacaa
     4021 tcgcgtcaag ggcggcagca agttgccggg tgccgcagcg agtgtgacct tcgccacggc
     4081 caataccaat gccaccttgc tcgcaaccgt gacgatctcg tgggatcttc cgggttcaag
     4141 cggcagggtg ggtgactcgc tgcacggggt ttatgtgacc caggccatgc ttttctgagg
     4201 aatcagcatc atgcgtcgcc cgccctttcc cgttctccgc gaccgctcga ggcagcgggg
     4261 tatcagtctg gtggagacga tgatcggcat ggccctgggg ctggtggcga ccttcgtgat
     4321 catccagtcg ttttcgtcca gcgagacata tcgccggaat ctcgccggaa cggccgatgc
     4381 aacccagacc gcggccattg ccggcggaca tctggatctc gtgctgcagg aggcgggcgc
     4441 ctccctcgtg caggggcgga acgtatgggg gtgcaagatc ctggtgaccc gcaacaagac
     4501 cgccatcctg ccccatggct ccaattatcc gagtccgttt gcgagctttc ccaccacggt
     4561 acgggtgctc ccgctggccg tgctcgacgg cggggacaag gggtccgacg tgatcatggc
     4621 catggtgggt aattccggaa ccggcaatcg cgataccccg ttcagtgcgc cggcgggtgg
     4681 aaagtccatg gtggtgtcga acaataacgg catggcgctg acctcatccg gtcagtcacc
     4741 gaacgatctc ttcctgtcgg tgccgcagga ggtggcgggg gcgccggggg actgtcaggt
     4801 cgtgcaggcg gacagcagct tctccaacgg cacggcggtt gccgatccgt ccctggggct
     4861 tcaggtgatg ccggcgtcgg tgacggcgcc ccagtccctg acgaccatcc cgctcaatgc
     4921 gtcaacctac ggggcgctgg cgtcttccgc caattcgcct tcggccttcc acctcgggcg
     4981 ggagtccgcg ccgatctttt ccctgttcgc ggtcaacagc aacggcgaac ttgtcgagta
     5041 cgacctgctg gaacggaacg gggtcatgac cttcggtgag aacatcttcc ttatcaaggc
     5101 ccgttatgga ctcgacaatg gggccaacgg aggcaccgcc aacgacaacg tgatcgacga
     5161 ctgggtttcg ccggccgaag acggctggaa gcttgcccag ttgatggatg gcaagagttc
     5221 gaccgagcag aaggtcgatc agatcaaggc ggtccgtctg gccctggtga cgcggtcatc
     5281 ccagtcggtg acctcggatg ccaaggtgag ctccctgaca ctcttcagcg acctctcgtc
     5341 cacgcggcaa tacacccgct ccctgagcga ctcggaacag gcctatcagt accagatcgt
     5401 ggactgggtg attcctctgc ggaacatgaa ggcgacgccc aagtaacgga tgcggccatg
     5461 acgacttcca agattcctct tcatccaccc cgccgcaatc gcggagccgt gctggcgatc
     5521 gtgctcgtcg tgctggtggt gatgatgctc ggtgccgtgt ccatgcttcg gtccgtcgat
     5581 acgtcagcct tgctgtcggg gaacctggca ttcaagcggg agtccctgaa tcgatccacg
     5641 gtgggcctca ataccgcctt tgcgcaattc aagaaggacg atttcgtcaa gtattcggac
     5701 gacagcaccg ggtgcaatgc cgcgtcgacc gggccgtcgt gcacctacgc cagcaattgg
     5761 ctgaagatga attattcgcc gcggctgctt gcgacggatg ggaacggtgt gccgcttatc
     5821 ctcaaggaca tcagcagctt cgacaaaacg ttcaacagca gccttgtcgt gacggacaag
     5881 gccgacccga acgtgaaggt gcgattcgtc atcgagcgca tgtgcaatga cttcggtccg
     5941 gccaccgaga ccaagtgcat cctttccgaa aatgccgaac gcgggggatc ccagcccctg
     6001 gacaagctcg gctcggcggc gctgccgatg taccggatca cggtgcgcag cgacggcgtc
     6061 cgcaattccc agacttatgc ccaggcggtt gtgaccaccc gcgtgaagtg acgcggccgc
     6121 gttgccgagg aagtgccatg aaactgtcga aaagcctgtc ccgattcctt ctgatcgtgg
     6181 cgagcgcgag cgcgctcttt tccgctggcc tgcaggacgg acgcgccgca agcacaccgc
     6241 ttgccgacca gccgctcctg acggcacagg tgccgtcgaa cgtgatcctc gccctgtcgg
     6301 tggaatttcc gaccgccctc agcgtggcct acaacacgaa attttctgtg gacgggtccg
     6361 cctacctggg ctatttcgat ccaaagaaat gctacaaata cgattcggcc aacaattatt
     6421 tcgtaccgca ggggccgagc ggtacgaatt acacctgtac gagtgcctgg agcggacatt
     6481 tcctcaactg ggccacgatg caggccatcg ataccttccg ctgggccatg accgggggca
     6541 accgggtcat cgatctgccg gtgaatttca ccgacaccca gccggtcaag acggtcctgc
     6601 agcgtgccta cgcgtcctcg gacggcagtg aatgtacgta tttccccggc cgtggcatca
     6661 gcgcgagcga ccgtgcgaat tacacggata cggcgggggt gggatatacg gataagggcc
     6721 tctggatccg caacggcggg cagggatatc agtttcaggt ggcgccgcag atcggcggcg
     6781 ctgcaaattg cagttcgtgg ggcggcggca gtggttcggt tcagtcctat tacgcccagg
     6841 tcgaggtctg caagaacgcc aacggttcgg atggcaagac cctgctggaa tccaattgca
     6901 cgatgtacgt ggatcctacc gggaccaaga cggcctatcg cccgatcggg ctgatgcagc
     6961 aatacaagct ggccatgaat ttcggagcct ttggctacct ctacgatggc agcagcacgc
     7021 gggatggcgg cgtgctccgc gcgctgtcgc ggcccatcga cctggagatt tcggatacgg
     7081 gagcgtttta tccggatccc ttcgggctgg ccaatgcgag ccgcggcatc atttacagtg
     7141 gtacggccaa ttatctcaac aaattcggca gtacgtccct tcactacaaa tcgctcgatc
     7201 cggtcagcga aatgtatgcg gaggtgatga agtatctgaa ggggatggat gtaacaccgt
     7261 cgtatgtatc gaccatcaac aattcgatgc gggacgggtt cccggtgttc ggtgagtctg
     7321 gcggtccgac ctgggttgat ccggcgcggg ataccagcga tccgtcctac ccgaacagtc
     7381 cggcgctgtg gtgcaagaaa agctacatca tcgggatcgg cgataccaat acatggaacg
     7441 accagaacct gtcgggacgg acggacgtgt cggtgccgag cggtgttgat accaatcttg
     7501 ccggcggcac ggcaaaaagc tggaccgata ccgtcggaac gaccgagggc tatgtcccca
     7561 gtggctacgc aagcctgggt gtggcgcagc cgaatcctgg ccactccaac agttttctga
     7621 tggcgggtat tgcctattac gcccacagca gcgacatccg cacggatctg aaggacacgc
     7681 agtcggttga tacctactgg atggacgtca tggagggcgg gatctacaag gacaagaacc
     7741 agttctggct ggctgccaag tacggcgggt acaagaagcc gctcaatacg gcgatgggtc
     7801 cgttcaactc gtccaccgat ttctggaaat cacaagggaa ggtcatccag actaagagcg
     7861 acgggacggt cgtcacgacg ggtggagcca agtcttatga cctgccggac aactatttcc
     7921 cggccaacaa cccggcgaac atggtctctt cgctgcagag cgcctttgcc cagatctccg
     7981 ccgggacggg tgccgcagcg ggggttgcgc tgtccaatcc gcaggtgaat gccgcaagcg
     8041 gcggttcgat gacgttccag gggacatacg acgcaagcac ctggtccgga aacgtcacgg
     8101 catcgaatat cacctcattc aaggatgatg gcacgccggt gaccacgtcg gtctgggacg
     8161 ccgcggccag attgaattcg atggttgcga gcgggggata cagttccaag cgaaaggtgg
     8221 tgaccctggt tccgaccaag gtcgataacg cgacgcccgc ggttgccgat ctggtgggca
     8281 agcctttcct gtacgccaac ctgagcacgg cccagaagct caacctgagc aagacgtcgc
     8341 tcggcggcga tctcggcgat ggagacaagg tactgaatta cctgcgcggt gaccgcacct
     8401 acgagaccac cgccacgacg cccaatcttt accggctgcg gggttcggtg ctcggcgaca
     8461 tcatcgattc caagatcgtc tatgtggcgg cgccgaatgc ggtgtattcc gattcgtata
     8521 atcccggcta cgagaccttc aagaagaatg ctgcgtcccg gcagcccgtc gtctatgtcg
     8581 gcgccaatga cgggatgctg catgcctttg acgcgaccag cgcgtccacg ggcggtgatg
     8641 aactctttgc cctggtgccc tacgccctgt atgccggtcc cagcaatacg ccggatgtgg
     8701 atggcctgca ggcaattgca cgaaccagct attcccacca ctattacgtg gattccacgc
     8761 cggaggttcg cgacgtggat ttcatgcggg cgggtggcgc gacccagtcc gatgcaacca
     8821 agagcgactg gcggaccttg ctggtggccg gtctgggcaa aggcgggaag agctttgtgg
     8881 cgatggacgt caccagcatc tcgtcgggcc tgaccgagag tcaggtggcg ggcaaggtgc
     8941 tctgggagtt cttccatccg gacatggggt tcagctttgg ccggcccctc atcaccaaga
     9001 cccgcaagtg gggctgggtg gtgatcctct ccggcgggta taacaacatc ggcggcgata
     9061 ccaaggccgg ttacaagcct ggtcagggcg tcgtcttcgt cgtcagcccc aaggacggca
     9121 gcctgctgca ggcgatctac acaggggctg gcgatgcggc aagtccggcc ggcctggcgc
     9181 agatcacggg ctatacccag aactaccagg actacacgac ggattatgtc tatggtggcg
     9241 acctcagcgg caatctctgg cgcttcgatt tcaccgacag cagcaagagc gtgccgtcgc
     9301 ccacgaagat agcccagttc aaggatcctg acggtacgcc ccagcccatc accgtggccc
     9361 cgaaggccga atattcggct gacgatctga agcgctacgt cttcgtcgga accggccgcc
     9421 tgctggcttc cagcgatcag aagaatgccc agaagcagac gatgtacgcc cttcgggatg
     9481 gcacgcgcac ctatgcgttc ggatccaaca ccaatcaggt ggccttgccc gacggtgtga
     9541 gttttccggt cggtcgcggc agtatgagtg gagtcagcga cctgcttgcc ggggcgacga
     9601 agtccgagag cacgccgatg gggtggtatt acgatctcac gggtgtcacg agcggtgtca
     9661 ccgagcgggt cgtgctggcc ctgcaggcca atgatggcgt gctgtcctgg gtcggctccc
     9721 tgccgaacag cgatccgtgc agtccgtccg gggcatcgcg cctgtattcc gtgcaatacg
     9781 gtagcggcca gagcgtcttc tatgaattgg tcaatggcct tcgttcgccg gttgcgtacc
     9841 tgagcatcga tcaggggctg gctgacatca cgctggtgcg ttacggctcc agcatccggg
     9901 tcgtcggaac ggataccaag gggggggcca aggtctatgg caacacgatc gccgagtcgt
     9961 ccgatccgcg cgtcgtgaac tggcggatca tccgtgaata gggcgcccgc gtgcggcagg
    10021 acttgaaccg gtaagggcgg atgacgtaag atgcgcgtcc tcgctgctgt agctcagtcg
    10081 gtagagcaac tgattcgtaa tcagtaggtc accagttcga ttccggtcag cagcaccaga
    10141 accccgtcaa cggctcccgc aagggggccg ttttcatttg ggcccgtccc ggaccggttc
    10201 agggggaaat gtgataaaac ggcgtgtccc gtcgggcccc tcgggtggct gacgaccgtc
    10261 tgctgtgagc gaggccatcg ttgtcccatt ccgtaccccc tcaggaacac gcctgctatc
    10321 actgcggact ccccatccct tccgacgttc atctcggcgt gacggtggca ggtgtcgagc
    10381 ggtccatgtg ctgtaccggg tgccaggccg tggcccagtc catcgtggat ggtggtcttg
    10441 tcgattacta tcagcgccgc gacgccatgc cggaacccgt gcgggaggcc atgccggacg
    10501 aactgcgcga tctggggctc tttgatcacc cggatttcca gcagggtttc gtcaagcccg
    10561 tcggtgagca tgaacgggaa gcgtccctga ttctcgaagg catcacctgc gccgcgtgca
    10621 tctggctcaa cgagcagcat gtcgcacgtc agccgggcgt catggcggtc gagatcaact
    10681 acgcgacccg gcgtgcacgg gtgcgctggg acgaacgtcg catcaagctc tcgaacatcc
    10741 ttgcggcgat tcaggccatc ggttatcggg cctatcccta cgatgccgcc cgctcggagc
    10801 agatctccca gaaggagcgt cgggcggccc tgtggcggct cttcgtggct ggcttcggga
    10861 tgatgcaggt gatgatgtac gcggtccccg tatatatggc cggagaaggc gagatgaccg
    10921 ccgacatcga ggccatgatg cgttgggcga gcctgatcct gaccttgccg gtggtgcttt
    10981 actctgccgc accgtttttc cgcaacgccc tgcgcgatat ccgcttgcgg cggctcggca
    11041 tggacgtgcc cgttgcgctc ggggtggggg ctgcctccgt ggccagctgc tgggccacct
    11101 tcgcgggcca tggcgaggtg tatttcgatt cggtcacgat gttcgtgttc ttcctgctgt
    11161 gcgggcggta tctcgagatg ttggcccgtc agcgtgccat acggggcgtg gaagagcttg
    11221 gcaaggcgtt gccggccttt gccgaacgcc tgacggcctt cccgtcgctg gaaggcgagc
    11281 gcgttcccgt ctcccagatc gtgtcgggtg aagtgctgcg ggtgcggccg ggggagacca
    11341 tcccggtgga tggtgtggtc atcgagggga cgagcgaagc gaacgaggcc ttgctcacgg
    11401 gtgaaagccg ccccgtaccc aaaggcccgg gcgtggatgt caccgggggc agcgtgaacg
    11461 tgtccagtcc gttgctcatc agcgtcaccc agacaggcga gcacacccgt ctggcggcga
    11521 ttcggcagtt gatggaacgg gcatcggcag aaaagccaca ggtggttcag caggccgaca
    11581 aggttgcggc atattttgtg gtggcgttgc tcgtccttgc gtttgccacg gcggtgggct
    11641 ggtggtggtt cgatccagac cgggccttgt gggtgttcgt gtccgtgctg gtggtgagtt
    11701 gcccttgtgc gctgtctctt gccacgccgg tggcgctgac ggtggccacc gatgccctcg
    11761 ctcgcgttgg cgtgctggtc acccgtggcc atgccattga ggcactggcg aagaccgagc
    11821 agatggtttt cgacaagacc ggaaccctga cctatggcga catgcgtgtg gaagcgacct
    11881 gtctgctcgg ctcccacagc acgcgggatg tacagcgact ggccgccgcg ctggaacagg
    11941 gatccgagca cgccatcgca cattcgctac ggaaactggt gacggacgca ccggatcaac
    12001 tgcccgttct gtccgacgta acggtcgtga ccgggcaggg tgttggcggg aggctcgaca
    12061 gtgagcgggt tcggatcggc cgcccggaat tcgttgcgga aatctgccct gggaccttac
    12121 ccgccgaact gacatccgcc caagcctcgg gcaagacggt cattgcactt gggggggagt
    12181 caggctggct ggcaggcttc ttcctttccg acgtcctccg cgaggatgcc cacgatgtat
    12241 tcgacgcgct gcacggggac ggcgtgacca ccgccatctt cagtggcgac acgcagggcg
    12301 ttgtctccga actggggaag gcgctgggcg tcaccgaggc cgtcggagag cttacgccgg
    12361 aaggcaagca cgttcggctc agtgccttgc aggcccacgg caaggtcgtg gccatggtgg
    12421 gcgatggggt caatgatgcg ccggtgctgg ctcaagccca ggtgtccatc gccatgggag
    12481 gtggaacgga tctggcgcgg aatcaggcgg acatcgtcct gctgagcgga cggctcgggt
    12541 ccttggtcga tggcatgcgc ctctcacgtc gtgcactgcg tatcatccat caaaatctgt
    12601 ggtggtcctt tgcgtataac ttcaccgcgg tgccgctggc gatgtcgggc tttgtgacgc
    12661 cgtggatggc tggtctcggc atgtcggcca gttcacttct ggtcgtgctc aatgcgttgc
    12721 ggctgcagcg caagcacgga aaggttgcag cctgatggaa agtctttacc tgctcattcc
    12781 cctgtcgatc gttttggtct tcgttatcgg tgtggttttc tggtggtcgg ttcgcagcgg
    12841 tcagttcgac gatctggaag gaccgggttt ccgtgtcgtg accgacgacg acacgccgcg
    12901 gacggagacg gagagccccg tggagcgcaa gggtacgacc gagcggtcat gaccagggcg
    12961 atggattcac cctgcaagct gaatggtttt tgacgcaggt caagaccccg tacagaagag
    13021 atgggcatac ttcacgccaa gttgatgacg cgaacacgag caacaaaatg cgttgtcagg
    13081 gcccgtcggc ccgatgactt aagtctctct gttggggttg ggggtgcgcg atgcgcaccc
    13141 ttttttttcg tccccacggt cttgaagccg acttgatgca tctcgcgcct gcgcgtcagc
    13201 gttccgaaat tcgtggcttg atgccggaat ccctgaagaa gatatgcacg gtttgaatgc
    13261 gtactctccc gagcgtgagc cctgcggtaa agcgcggtgc gccagtggct tgcaaagggg
    13321 gcgggcagtt ctgatggtga ggggaccgtc cgcccgcacg accatctctt cgccggatca
    13381 aggtgcacgg ccggctcttt gcggtgctcc gggcgcgccg cgggaggctt tggaggggtg
    13441 tcgctcccga gtttcctgac ggttggcagc gccttgtttt ttcggccatt tcggtgcatg
    13501 cctcacgcgt tttgcacccc cttggtgcgg ttgcgaaaac attcgacggc gcccgcgggc
    13561 cttgctattg tgtgaattcg cggggcattc cgacccgcct tcaggcacat ggagcgcgtc
    13621 aattgggtaa caggctttac gtgggcaatc tggcctacag cgtggacaaa acccgtcttg
    13681 aagagggttt ccgggagttc ggcacggtcg tgtccgcaca ggtgatcatg gatcgcgaaa
    13741 ccggtcgatc caagggtttc ggtttcgtcg agatgggctc ggatggcgag gcccaggcgg
    13801 cgatctcggc gatgaacggc aagagtttcg atggtcgcaa cctgaccgtg aatgaagcgc
    13861 gcccacagga gccccgctcg ggcggtttcg gtggtggcat gcggcggagc ggtccgggag
    13921 gtgggcgccg gggcccgacg tcgggtggtg gcgaccgtta ctgatcgcgc gcagccggcg
    13981 tgaagtgccg gctgcaagag ttcctcttcg tgaggtaggc tcgccgaccg atccacccgg
    14041 gtgctgcgct caggcctgcc cgggagcatg tccgagccgc cagacacatg acgtctgcgc
    14101 gaagcgccgg ttagcccggc tcaggtgttc tcgaattctt ccaccatcaa ctgtacgctg
    14161 gccacgccgt tgtattcgtt gacggccagg cggaaggcgg cgtggatggc tgcgcccgcc
    14221 ggatctgcgt gattgaagcg gatggcgtcg atgcgcaggg cacctttgcg caattgcagc
    14281 ttgaggtgct tgtccttcag caggcgctgg ttttccaccc ggaagcagtc gtcgaagagc
    14341 ggcgccggaa agccctgccc ccagatctgg ttttccagca ggcgcgctgt ttccagactg
    14401 tagaacccct cttccagtga tccgtcggtt tccacgctgc gggtcaggtc ggccggatca
    14461 atcatctgac ggacgacgcg ttcgaacgca tcacggaacg ggcccagatc cggctcgcgg
    14521 atcgtgagac ctgcagccat tgcatggccc ccgaacttga ggattagatc cggcgcctgc
    14581 ttggtgacga gatcgagggc gtcgcgcagg tgcaggccgg gaatggaccg gccggagcct
    14641 ttgagttcac cctcatgact gcgggcgaag gcgatggtcg gtcggtggaa gcgttccttg
    14701 atccgcccgg cgagaatgcc gatgacgccc tgatgccagt cgggctcaaa caggacgagg
    14761 ctgctgcaat cgcccggcga gaggccatcc agtccagcca gggcaccttc ctgcatgtcg
    14821 gcttcgatgg cacggcgttc ccggttgagg gcgtccagtt cctgggtgat ctgcagggcg
    14881 cgactcgcat cgtcggtgat gaggcattcg atgccgaggg acatgtccgc cagacggccc
    14941 gcggcattga gtcgaggccc cagcgcaaag cccaggtcga aggtggtggc gcggccggcg
    15001 tcccgtcccg cggcggcgaa cagcgcgcgc aggcccggtt gcatacgccc tgcgcgcatg
    15061 cgttgcaggc cctgggcgac gaggatgcgg ttgttgtggt ccagcctgac gacgtcggca
    15121 acggttccca atgcgacaag gtcgagcaga tcggcgagct tgggctctgc ctggccatca
    15181 aaggcgccac gtctgcgcaa ttcggcacgc agggcgagca tcgtgtagaa catgacgccc
    15241 acaccggcca gtgccttgct tggaaactcg cagcccggct ggttgggatt gacgatcacg
    15301 tccgcctcgg gcagttcgtc gcccggcaga tggtggtcgg tgatgagggt ggcgatgccg
    15361 cattcgcgtg cggccgcgac gccgtcgaca ctggcaatgc cattgtcgac ggtgatgatc
    15421 agttccggct gcagggcggc cgccagttcc acgatggctg gcgtgagacc gtagccatac
    15481 tcgaaacggt tggggacgat gtagtgcacg tcggccccga aggcccgcaa cgcgcgcatg
    15541 ccgacggcac aggctgtggc gccgtcacag tcgtaatccg ccacgatgac catccgcgcg
    15601 ttggcttcga tggcgtcggc gaggagctgc gcggcggcac cgacgccttt cagctggttt
    15661 ggcgggagca ggtgctggag gccgtaatcg agctcatgac gggtcgcgat gccacgggcg
    15721 gcgtagatgc gcgccagtaa gggatgtacg ccggtatcga ggagggcggt gacggcctgg
    15781 ccgggggccg agcggctgag gatgcgggtc atggcgcggt ggtggaggcg acgtccgagg
    15841 cggggttcgg cgtttggcgc aggagggtgt cgagggcgag gggcttgcgc cagaatttcc
    15901 agaggctcgc ggcggtgacg tccagttcaa ggcaggcccg gtcgccaggg gcggtgatgc
    15961 gtagccggct caggtgtctg gacctgagcg cggccagcag gggcgcgaac cagtcccgtt
    16021 cgatcgtgcc gagcgtcgcg cgccaggcgt ccgggtcccg gtgcagcagc ggacgccgaa
    16081 gggcggtgag cagcgtgaag ccgtcggtgc ccggcgcggc gtacggtccg gacgaccggg
    16141 gcgcgccgcc gctcgcccgg atcagaccac aggtgtaggg gtcatcggcc tgaaggctgg
    16201 gcgctggtgc aatcggcgta agcggctgcc cggcacccca cagccatgcg ctgttgatgg
    16261 cgcgctgtcc ggccgcttcg cgggctgcgt tgacggggtg gttgaatagc cacacctgga
    16321 gttcgttgct caggcgcgcc cagaacggaa tgtcgtcacc ttccggcaga aagagctgga
    16381 tgggacgtcc cgtcacgtcg gccaggggat ggaagaatgg acgcggctcg cgctcggcac
    16441 gcagatacca gcggtcgggc gccggagcct cgaagcgacc gatgtcggca aaggtctcgt
    16501 tgaggccggc gatcaacgtg tcggcctctg cttgggtgat gtccagaccg ctggcgtcgg
    16561 cgaggagcag cttgtcccgg gcgaaatgca gatgaaccgg gtctatgcac aaccagtggg
    16621 cgctgcgttc cgggcttgtg gtggcttcgc cgaggcgtcg caaccaggca tagggaatac
    16681 gcttgccggc gagctggaag cgatcggcga gccacgcttc aggtgtgcac gctgcacccc
    16741 aatgcacgcg ggcgtggccg atgagggctg ccagggccgg gagctcaagg tcggcggccg
    16801 gtccggccag gccggaagcg ggccacacga ggccgggaag gacgaggtcg tactgcatcg
    16861 ggtcgagaat gggtgggcta cagcggcgcg ttgggcaggg cgcaggcggt cgggcgcgag
    16921 tctagcatgg gctctgcggg ctcagggttg ccgcggctcg atgagtgcgg aagcataggc
    16981 ggccgagcag cggagggcct gttcgggccc gaggttgcgg gtttcggcca gcaggcagcg
    17041 gggatcattg tcaccttcga ggacttcggc gggcagatcg cgcaggctgc gggcatcgat
    17101 gggcgtggcc agaatggcgc ccgcggcctg gaagccggcc cgcacgaatc gccgcggcgc
    17161 gtccgtgacg agcaccagca ggggcccatc cggcgtgccg gcgagggaag ggctgatctg
    17221 cacgccgggt gccagatgca gccggctgtc cgaggcatcc ggcagcagca gctcgaaccc
    17281 gcgcccatcc gatggcgcat gcaggacgta gaggaaacct gcccgcggtg agcggatgag
    17341 gagtctgtca tcctgccggc gtacgtcgag cggtcgatct gcagcccgct gggcatgcag
    17401 gatgcggagg agcgccgcgg gcgtaaagcc gggcgctgca gcaggggtca gcgtcggaag
    17461 gggcacaagg tcggcattgc caacccgttc gaggcggcgg gcgcccgggc gcgcgggacc
    17521 gagttgtgcc tccgcgcagc gacgcaggtc gtcgatcctg gcaagtccac tcgtactcgt
    17581 cacgggggca tcgcccgaca ggcaggccag cagcgccgcg ctggccgcac cgaagtccgg
    17641 actgtcggag gcaagcaggg cgagattggg gggggagttg ggcaaggctt cttgccagtg
    17701 ctgaaggagg ccgtgactgc gggacggcac atccgttcgt ctgtccgggg ccatggtctg
    17761 cggggcgggc gaggctgctt cgacgacgat cagcacctgc ccggccttgc gggcgagcgt
    17821 gtcgaggtaa cgacccagtt cggcgacctg gacgcgaccg tcaggggtgg ccagggccgc
    17881 tgcggacggg gcccgggctg cgcgcggtgg ttcggtacgg gcgaggatgt gtacgaacac
    17941 ccggtcgccg gggccggcgt gtgcgtccag atcggcgagg gcacggcgga tgcggtcggg
    18001 ggtgaggctg acggggtcgg gttgctggat gttggcatcg gggacgccca gtgcccgggc
    18061 gagctggcgt gcggtcagat cggtcgcatg cgatccgggc gggacggggt cgtgatcggt
    18121 caggtccagg agcagggcgt gacgactggc gtggggctgg aggcggggac gggcaccgct
    18181 tgccgcaaac gcggtcggcg cggcaggggc tgacggactc gccgggggtt cgtcgaggcg
    18241 caggctggcc gcggggaccc aaccccggcg gttgcccgca gcatcgacca ggtaccagtc
    18301 gcgccgggcg tcggcgatgt ccacgctgct caggcggtgc agcggcatga cgatggcggc
    18361 gtcgctgcgg ggctgggcgc gcagggcgtc gtcgtggatg acggtggcac cgacagcgtg
    18421 gacgataccc gtggcgagca gcagggccgt ggcgaacgcc ctgtgaaagg cggggaacct
    18481 tggggggtga aaacgggtca ttggtgcgtc gcgccgggcg gcgtccggtg gctgaaggtg
    18541 ccattctgac ggtccggggt ggccggtgcc agccccggac ggtgcgggcg ctgtcccgtt
    18601 tcccgaccaa gtccggcaca atgcgtggct gcgccggcgc gaggcgtttt tttctggttc
    18661 gttcatgtcc tacgaatttc tggtcggtct gcgttacacc cgttcgaagc ggcgggcccg
    18721 caagggaaac cgcttcatct cattcatctc cctggtgtcg atggcgggca ttgcgctggg
    18781 cgtggcagcg ctgatcgtgg tgctgtcggt gatgaacggc ttccagcggg aactgcgcac
    18841 gcgcatcctc ggtgttgcgt cccacgtgca ggtcaccggc ttcgacggcg agctggccaa
    18901 ctggcagcgc atcgcggacg aggcatcgaa gcatccgcgg gtgctggccg cggcgccctt
    18961 cgtgcaggcg caggggatgt tgtcctttga tcagacggtg cgtggcacgc tggtgcgggg
    19021 catcctgccg gatgcggagg acaaggtggc ggatttcgcg cagcacatgc gcgccggtcg
    19081 gctcgacgaa ctccagccgg ggggcttcgg tatcgtgttg ggcgccgatc tggcgcgcgc
    19141 gctgcgcgtg accgtgggtg acaaggtgac gctcatcgca cctcagggcc tggtgacgcc
    19201 ggcggcaatc ctgccgcgcc tgaagcagtt ccgggtgacg ggcatcttcg aggtcggcat
    19261 gtatgagtac gactccagcc tggccttggt ccatctgcag gatgcccagg ccttgtaccg
    19321 gatggaagac cgggtgtccg gcgtgcgact gaagctcgac gatctgttta aggcgcccca
    19381 ggtgtcccgc gagctgatgg gctacgttca ggcggatacg ctgatttcgg actggacccg
    19441 cgcccatgcc aatttcttcc gggcggtgca gatcgagaag aacgtgatgt tcatcatcct
    19501 gctgctgatc gtggcggtgg cggccttcaa catcgtgtcc acgctggtga tggcggttca
    19561 ggacaaacgg gcggacatcg ccatcctgcg cacgctgggg gccagtccag gttcgatcat
    19621 gggcatcttt gtgatccagg gcgcgctgat cggggtggtg gggctcgcca tcggcgtgat
    19681 cggcggtgtg gcgctggccc tcaacatcga cgtggtggtg ccggccatcg agcggacgtt
    19741 cggcatccag tttctggcca aggacgtgta ttacatctcc gacctgccgt cgcaactgct
    19801 gtggagcgat gtcggcgtga ttgcgaccgt gtccttcgtg ctgacccttg ttgcgacctt
    19861 gtatcccagc tggcgggcat cgcggctcaa cccggcggag gccctgcgct atgaatgaac
    19921 ggacgtccga tggggtgatt gcctgccgtg ggctcgccaa gactttccgt gaaggcgatg
    19981 acgcggtgag cgtgctgacg gacgtcaccc tctccgtgca acggggcgag cgggtggctg
    20041 tggtcggggc ctcgggctcc ggcaagagca ccctgttgca tctgctgggc gggctggatg
    20101 tgccgtccgc ggggtcagtg agtctgctgg ggcgggattt ctcgacgatc tccgatgcgg
    20161 agcgggggcg cctgcgcaac cagtcactgg gtttcgttta tcagtttcat catttgctgg
    20221 cagaattttc agctctggaa aatgtggcca tgccgctgct ggtacggcgg ctgccccgtg
    20281 aggaggcgct ggcgcgggcg gccgaggcgc tgaaggacgt cggactggaa caccggatgg
    20341 atcacacccc cggcgagctg tctggcgggg aacggcagcg cgcggccatt gcgcgggcgc
    20401 tggtgacccg gccgacctgt gtgctggccg acgaacccac cggaaatctg gaccgccaga
    20461 cggcggaacg ggtgttcgga ttgatgctgg cgctggcgga gcagcatggc acgagtttcg
    20521 tcatcgtgac ccatgacacg gcgctggctg cccggacgca ccgcgtcctg cacctgcggg
    20581 acggtcgcct cgcaggggaa acctgactcg gcagcggggt cttccgcgta cggaaacatg
    20641 acatcgtggc agaaggcgct ctcaagtttg tgggcgcggc accgatagag tgcataataa
    20701 ttctgttgaa ttaaatgttt ccgacgattc aaaagggaga tgaacatgca cctgctcttt
    20761 gcgccggccg tcgcattgct caatcgaatg cgataccggg ccaagtttgc gttgctgggc
    20821 gctctggcct ttgcggtggt gctcttcctg ttggggcagt tggcgttttc gctgcgggaa
    20881 agcatgcact tcaccgaagc cgaacggtcc gcgctgggcg tggtgccccg cctgctgacg
    20941 gtcatccagc tgacgcagca gcaccgcggc ctgtctgccg gagtcctcaa tggcaatgag
    21001 ggcatgcgca tccgcctgac cgccaaaacg ggggaactgg gtgaggccat gaaaaaggtc
    21061 gatgccattg cacttggcgc ggacgcctcg attccgatgg cacggtgggg ggaagtgaaa
    21121 tccgcgtggg aggtcctgcg agccaacggc ttgaatcttc cgccgaagga aaatctcact
    21181 gcccatagtc gcctggttgc gatggaggtg ctggcgctgc acgatctggg cgacgacggc
    21241 aatctgaccc tcgatccggt ggcggatacc tattacctga tcgacaacct gctcaagcgt
    21301 gttcccgaag tgacggaacg tctcggccgc ctgcgtgcgc tgggcacagg agtgctggcg
    21361 gccaagagcc tcgatgagca gaagcggtac gacatcagcg tgcagttggg ggagctggac
    21421 atggcggtcg aagccctgaa cgaaaacttc gaccgggcca tccgtttcaa tccggcgctc
    21481 aagggatcgc tcggtgcctt gcgtgatggt ttcaatgcgg gaaccggcaa ggtgctggac
    21541 cagttgcaga atcatgtgct caagggggat ttcgagatgg cgccgcaggc ctatttcgat
    21601 ctggtcactc aggcgatcga catcgcgtat gccaagacct tcggagagtt gatcccggcc
    21661 accgacgagc tgctggccgc gcggattgcc ggtcatcagc ggagtttcgc catcgatctc
    21721 gtggttgcgg tcctggtgat cctggtgctg gcctacctca gcggcgcgac gtacgtctcg
    21781 gtcaatgatg cggtgacccg cctcagcaat ggcgcggaat cgctggcgaa tggcgatctt
    21841 tcaacgcgca tcggtctgga aagccgggac gaacttggcg acgtcgccga gcgtttcaac
    21901 gcgatggcgg ctgcgttctc ggaagtcatc cgcaaggttc agcaaggagc cgacgcggtg
    21961 gccgggtctt ccgtctcgct ggcgggatcg gccgcccgtg tgtcggatgg gtcggaacag
    22021 caaagcgcgg cggcgtcgag catggccgcc gcggtggaag agatgacggt ggggatcgag
    22081 gagattgccg tcaacgccgc atccgcggaa ggcgtgtcgt cggagtcggg gaaattgtcc
    22141 caggaagggg gggcggtggt ggcgcggacg gtttcggaga tggagcggat cgccgacgcg
    22201 gtgcaggaat ctgcccgcgt gatccaggaa ctcggcgacc agtccgcgca gatctcgacc
    22261 atcgtcaatt ccatcaagga aattgccgac cagaccaatc tgctggccct caatgcggcc
    22321 atcgaggccg cacgggcggg cgagacggga cggggtttcg cggtggtggc cgatgaggta
    22381 cgcaagctgg cggaacgtac ggccaaggca acggaggaga tcacgaccat ggttgcgtcc
    22441 atccaggccg gcacgggcaa ggccgtggac accatgcagg atggcgttcg ccgtgtacag
    22501 gaaggcgtcc agctggcggg ccaggcgggt atttcgatgg atcagatccg cagcggtgcc
    22561 gggcgggtgg tgactgccgt caccgatatt tctgccgcct tgcgggagca gggggtggcc
    22621 agcaccgaaa ttgcccgcaa tgttgaacgc atcgcccaga tggccgagca gaacagtgcc
    22681 gcggtccggg atacggcagg caccgcccgg gaactggagc gcctcgcgca acaattgcgg
    22741 ggcgacgttc aacgtttccg gatctgagcg tctttcaaca gggacgacgg ggcgcctgag
    22801 gcgcccttct tacttgagcg acggaccgtc tgccgacgga cggcggatct caatgcggga
    22861 gcagggcggt ttccagttcc tggcggccga ggggtgaggc catcgcgatg acatgctggc
    22921 caggttgcag gcggaagtcc ttgggcggat tgaagacgaa ggtctcgccc tcgcgcacgg
    22981 ccagcagcac atagttcggg ctgcgtagct gcagggtacc cagatcgcgt ggttcgaagc
    23041 gcggcggcac ctgcacttcc tcgagacgca gtttcttctc ggagcgcagc atctcgtcca
    23101 ggaaggacac cacatggggg cggaccatgc tggacgcgat ccgcatgccg cccgtgaagt
    23161 ccggtgagat cacgctgtcg gcgccggcct tgcgaagttt ttcggcattg cgcacttcat
    23221 gacagcgggc caccacgcgg atcgacgggt tgagctgctt ggccgtgaag acgatcatca
    23281 gattgcggct gtcgtcgccc gtcaccgcaa acacgccctg ggcgtcctgg atgccggctg
    23341 acatcagcag gtcgtcgtcg cttgcgtcac cgaccaggta aaccaggccc gggctgcgtt
    23401 cccgttgcga ttccatgccg gcttcatcgc agtcaatggc gacgaagtgg cggttcgtgg
    23461 cggtcatttc gttgcccacg ttacggccga cgcggccgta gccacagatg atgaagtggc
    23521 cgtggagctt tcggatctgc ttttccatac gtcgtctccg caggctgtaa tcgaggtcac
    23581 tctcgaggaa gaagaccgtc aggctggtga acagaaaggt gacggcgcca aagccggcaa
    23641 gggcaatcag gcccgcgaag aggcgctcgg cgatgccgtc gatgggcacg acctcaccgt
    23701 agccgactgt ggtgaccgta atcagggtca tatacagggc gtcggacaga ttggcctgct
    23761 cgccgcccgc tgcgtaaaag cccaccgtgc caagcacgat caggcccagc agcacccccg
    23821 ccgcgaaata gatgcgcagc aggatgcgcg ggagctggct gcgccgcgac tgtcgcatcg
    23881 ggatgcgccg cttgggcgcc ggctgttgcc aggcagtgac gaactcgtcg agcttcatgc
    23941 gagccctttc cggcggcgcc aggcgcgcac cagatggatc cgccatgcca gccgggtcag
    24001 gacatagccg gaacaggcga gcacagcggc cagcgcgacg agccccagag ccaggggccg
    24061 tcccaggtct gcggcccagt gggcgagttg tccgagccag tgaaggagac tgccgtcaag
    24121 atcgggtggc gcgatgaacg tccggccgtc cggaccgagg atccactggc cgagggtgaa
    24181 ggcggcgaga tacaggggca cgatggtgaa tgggttggtg tagagggtcg tgaagagtgc
    24241 caacggcaga ttgaccctga acatgacact acagagcgcc gcgcccagca tctggaaggg
    24301 cccggggatc aggccgcaga acaggccgac ggccacggcg cctgcggcgg aatggcggtt
    24361 gagatgccac aggcggggat gaagcagggg agcgccccac cacgccagcc agcgatggtc
    24421 ggcgagggat tccggcgtag gcagatggcg cttgagcagt cgtcgcatgg tacgtcacgg
    24481 tgcgaccgtt ctggtcgaac cgggcgagcg ggaaaatctc ccccgccctg attctactct
    24541 acgcccggct tggtgtccgg ggcggagcag ggtagattcg cggtcatgct catgtcaatt
    24601 tccggcttcc tcgtgggtgt cgtgtcgtgc cagtttgggg cagcgcttcc ggcaccgtcc
    24661 tgccagtttg cagccgccgt actcgcggct ggctgtgggc tggccggtgc ccttttgtac
    24721 cgctcgagcc gggaacgatg gccagggctg gccctggtgc tgtcgatgct cgccggcctg
    24781 gcggccggtt acgcctacgc gggctggcgg gcagagttgc gccttgccca gtccctgcct
    24841 ggcgactggg aggggcgcga cgtggcggtg acgggcatcg tggcgacctt gccgcagaat
    24901 ttcgggcggg gtgtacgttt tgtgttcgag gtggaggcac ctgcaggggg ggctgtgccg
    24961 cgccgaattt ccctagtctg gtatcgcggc gctgacgacg acgaggccgg gctgctgggc
    25021 gaggtgcatg caggcgagcg ctggcacctg ctcgtgcggc tcaagcgacc ccacggaaac
    25081 gtcaatcgtc atggttttga ttacgaggcg tggttgctgg agcaggatct tcgcgcgacc
    25141 gggtacgtgc gggcagacgc gtcgaaccgg cggctggacg ccttcgtgca gtcgcccggc
    25201 acgctgatcg agcggctgcg ggaggtcgtg cggagccgct tccaggccgc cctgcctgac
    25261 gggcgttacg ccggcgtgct gattgccctg gccatcgggg accagcgggc gatcccccag
    25321 gagcaatggt cgctgtttgc acggaccggc attgcgcatc tggtttccat cagcggcctg
    25381 cacgtgacca tggtggcggc cttatgtgct gcggtgatcg gagcgatctg gcggcgcgtg
    25441 ccggccctgg cgctccgcgt gcccgcccag caggccgcgg tggcgaccgg cgccttagcg
    25501 gcggggggct atgctctgct ggccgggtgg ggcgtgccag cgcaacggac gtttgcgatg
    25561 ctggcgtggg tggcgctggc gctgctgctg cggcgggagg cagcgccgtt ccgggtgctg
    25621 ggccttgcgt tgctgggcgt cctgctgctc gatccctggg cggtgctggc gccggggttc
    25681 tggctgtcgt tcggggcagt ggccttgctg tttctggcgg gcagcggacg gcttgccgcc
    25741 ggcgggcgca tccgggaggc cttgcgcgcc caatgggccg tgacggtggg tatggtgccg
    25801 gcacttctgg tgctgttcca gcagtgctca ctggtgtcgc cgctggccaa tggcttggca
    25861 atccccgtgg tgagtttcgt ggtgacaccc ctggcgttgc tgttctcggt cttccccctg
    25921 cagatcctgg cggacgtcgc gcatgcggtg ctggggggcg tgatgctgcc agtggaatgg
    25981 cttggtggcc tgccctacgc ccagtggcag caggctgcgc cgcctttcgt gctggcgctc
    26041 atggccacgc tgggctgtct gtgggcgctt ctgcccgtcg gcactccggc ccgctggctg
    26101 ggggcgtcga tgttgctccc gctgctcgcc tggcaccccg cgcggccggc gccgggtgcc
    26161 ctgaagatga ccgtgctcga tgtggggcag gggctggccg tgcacctgca gacggcggcg
    26221 cacgatctgc tctacgacgc cgggccggcg ttttccgccg acgccaacag tggtgagcgc
    26281 atcgtgctgc cctatctgcg ggcgcagggt gttcgtcgtc tcgacagcgt ggtggtgacg
    26341 caccaggaca acgatcatgc cggaggcgcc gaggcggtgc tgggcggcat tcccgtggcg
    26401 gaagtacgct cttccctgcc gaccgcccat ccggcacgcc tgatggccgc ggaccggcat
    26461 cttccctgtc gtgccggtga cgcgtgggaa tgggacggcg tgcgtttccg tgtgcttcat
    26521 ccgggggcgg aggccgatgc ggatcgtccg cgcaagacga acaacctgtc ttgcgtgatc
    26581 agggtggacg ctgcggggca cagcgtattg ctgacctcgg atatcgaagc ttcggacgaa
    26641 gaggccttgc tggcgcggga tccaggggcg ctggccagtg atctggtgct tgccccccat
    26701 catggcagcc ggacgtcctc cacgccggcc ttcgtggcag cgacgcatcc ggcctgggta
    26761 ctgtttcccg tcgggtaccg gaaccgtttc caccatccca atccggaggt gtggagtcgc
    26821 tggagcggcg caggcgcgcg gatggcgcgt accgacgcgg aaggcgccct ggaggtcagg
    26881 gttgatgccg aaggggtgca ggtcgagggc gcgcgggcgg tggcgccgcg atactggtac
    26941 gggcgctgac gcgatcggca atccacgggc tgcagatgcc ggactgcatg gtgccaaagc
    27001 ttcacggtgc cgatgctatc cttccggcgt taccactctc gcccttgtcg gccgggtctc
    27061 catgagcttg attgatgccc tgcgcaggct gttcacgcct gcccccgtcg ttgtgccacc
    27121 ggccgcggag gtgtccgccg gaggcttcga gcagcctcga ttccaccgcg tccagtgtgc
    27181 cggcccccat ggcctgcatc gaatcgccta tacagagtgg ggtgacccgg ccaatccgcg
    27241 ggtgttgatg tgcgtgcatg gactcacccg caacggacgg gatttcgacg acctggcccg
    27301 gtcactggcc catcattacc gggtcgtctg tccagatgtg gtgggccgtg ggcagagcga
    27361 atggctggcg gtgaaggacg attatcagct gcccacctac gtggccgaca tgatcaccct
    27421 gatcgcccgc ctgaacgtgg agaccgtgga ctgggtcggg acgtccatgg gcgggctgat
    27481 cggaatgctc gttgccagtc tgcccgaaca gccaatccgt cgcctggtgc tcaacgacgt
    27541 ggggccggtc atcacggtga gctccctgcg tcgcatcggc gaatacgtgg gcaaggcacc
    27601 ggtgtttccc agctacgagg ccggcgaggc cttcatccgg gcggtgagcg agccgttcgg
    27661 gcgcctcagt gacgcccagt ggcgtcagct cgcggtccat tccctgaagc agaccgaggc
    27721 gggctgggag atgcgctacg acccgggcat cggcgaaccc tttcgcaaga ctccggtgct
    27781 gatggacgtg gaactgtggt cgatctacga cgccatcgcc tgtccgacgc tggtggtacg
    27841 cggggccgaa tcggacctgc tgctgcggga gacggccgtg gagatgggcc gtcgcggccc
    27901 gaaggcaagc ctcttcgagg tccctcacgt gggccatgca cccaccctca tggagccggc
    27961 ccagatcgcc ccgattcgcg acttcctgct cgggagccgc tgatgctggc gacgctggtg
    28021 agtgccttca gcgtggtgga ttgggccgcg ctggcgtggt tcttcgcctg ctggatgggc
    28081 tacggctggg tggtggagca tggccccaag ggcggcatgg gcggtttgct gggcgccagc
    28141 catcgcttcc gtctcgaatg gggccggcag atgctgcggc gcgaggtgcg ggtggcggat
    28201 tccgcactgg tgggcaacct gatgcagaac gtgtccttct acgccaacac caccatctac
    28261 gtgatcgccg gcctgctggc gctgctgggg acggtggaca agctgatcga tgcggcggcc
    28321 gagctgccct ttgcccggca cgcgtcacgg gaggtgtggg aggtgaaggt gctcctgctg
    28381 ctgatggtct tcgtggtggc ctacttcaag ttcacctggt ccctgcgcca gttcaacatg
    28441 ttgtcgatcc tgatcggtgc cgcgccgccg ccgggggagg gcagcgaggc agacgcccag
    28501 cgctttgcca aggtgaatgc gctggcgggc gatgagttca accggggcat ccgcgcctat
    28561 tacttcggcc tcgcggcggt ggcgtggttc gtgcaaccct ggctgttcat ggcggtgacc
    28621 accttcatcg tgctggtgct gtaccggcgc gactttgcgt cccacacgct ggcggcgatg
    28681 cgggagtgat cactccttgg gcttgagctt gaccgctgcg gagttgatgc agtaacgcag
    28741 gccggtcggc gcggggccgt cgtcgaagac gtggcccagg tgagcaccgc actgcctgca
    28801 gaggacctcg gtgcgcacca tgaagtggct gcggtccacg gcttcctcca cgttgcccgg
    28861 cgccgccgca ctgtggaagc tgggccagcc gcagccggag tcgaatttgt gttccgaggc
    28921 aaacagctcg gcgccgcagc acacgcaacg gtactcgccg gcgtcgtgac agtcccaata
    28981 gcgcccggtg aaggcccgtt ctgtgccttt ctggcgggtc acgtggtatt cctcgggatt
    29041 cagctgggcg cgccattcgg cgtcggtctt ctcgatcttg cgggacatgt gggcctccgg
    29101 tcggggtgtc cggatggacg tcgggcgggg ccgaaaggtt cccgcccgga tggggctcag
    29161 tgcaggtgct tgggcatctg ctcggcctgc tcttcaggga gctcggcgtg caccgcctcg
    29221 ccttccggcg agggatacat gggcgcgccg cagtcgtcgc agtattccat gggaaactcg
    29281 ttgtcgagca cctggatgtc ggtgatgccg caggcacgca gcgtcgcttc gatttcgccg
    29341 agggtctcgc tggtctcccc ttcggcaccg aggagcggcc agaccacgcc gtggaccacg
    29401 tcgttgctgt tgcgcagggt gaagccgatg cggaactcct ccacctgacg ctcgaagaag
    29461 ggcgcgacga cggcccgcag gcgcgtggcc ggggcatcca gcgtggtgct caggaaggcc
    29521 acggacgcct ggatcgagta ggggcgagag gccttgtcgg cttcgcggct ggcggcgaag
    29581 taggcattgg gcaacaccac ctcggtggca cagccggtga gcagcggggc gatgcaggcg
    29641 gcgccctggg cgcgccactg ttcgaaggcc tgatcgcggg tgcagtcctc ttcctgccag
    29701 cggaagatgg gcgcgccctt gggcaccacc acggcgccga ggatgtagcg ggtgtcggac
    29761 aggaactggg cggtttccgg caggttgtcg gtcttgatgc gcaggtcggc gtcggtggcg
    29821 gccgagcggg ccagatggga ggcgaaatcc gccgtggcgc agtaaccctg gggcagttgg
    29881 tccggactga acaggtagtc ggcgagggcg agcttgacat ccttggccag cacgtgggcc
    29941 ttgagctgga cacgcaggtt ggcgagggcc gcggccggaa tggtggcgga cggaatgcgg
    30001 taacgcgacc acgccatgac cggcgcggcg atgaggagga cgtcgtgggg atcgtcagag
    30061 gtgagggcgc cttcggcgcg ggattcgatc atgtccgcca gctcgtcgta ggcggcggcg
    30121 ttggtgctgc tgaggtggtc gagggcggtg ttgagggtgt cctcgtcctc ggcgcggagc
    30181 aggccgtcca cggccgcggc gaggcgctgg tcccagaagt gatcttcggc gcggctgctg
    30241 gattcggcca ggccgctggc gagccacagg agctgttcgg cgtcccgcgg caggccgccg
    30301 cggcgtccga gacggttacg tttcatcggg gctctccttg ttgtcggtac gatgcctgga
    30361 aggctgtttt ggaaggggcg gcgggcgtgc ccgccggggc cgtcagaacg acgaggggtt
    30421 gaggcggact tcctggagga tgattgtgga gatctcctcg atggacttgg cggtggagtc
    30481 cagccagcgg atgccttcgc gtttcatcat gcgttgggcc gcttcgatct cgcccaggca
    30541 gttttcgatg gaggcgtagc ggctgttggg ccggcgctcc tgacggatct gggagaggcg
    30601 gtcgggtccg atggtcagcc cgaacagctt gtgccgatgg cgggccagct cgccgggcag
    30661 cttgttgcgc tcgaagtcct cggggatgag cgggtagttg gccgccttga cgccgaactg
    30721 catggccaga tacaggctgg tgggggtctt gccggagcgg gacacgccca cgaggatgac
    30781 atcggcttcc tgaaggtcgg cgtgggagac gccgtcatcg tgggcgaggg tgaagttgat
    30841 ggcctcgatg cgacgggcgt aatcctggct ttcggcgatg ccgtggaagc gtccgaccgt
    30901 atgggtggag cgctgaccca gctcgctttc cagcggaccg atgaacttct cgaagagatc
    30961 gaggaaaagg gcgtcggatt cccgcagggc ggcaacggcg gccgggtcta cgagggtatt
    31021 gaagacgatg gggcgcaagc cggtttcgga tgccgcctcg cgaatgagca gggcgcagtc
    31081 gcgggccttt tcgagggagt cgatgaaggg cagccgacgg gcacggaagc gcgtgtccgg
    31141 aaactgggcg aggagactgt ggccgagggt ttcggcggtg ataccggtac cgtcggagat
    31201 gaagaatacg gggcgggtcg ggctcatgag cgatggtgac gaggaatgcg ccgggggcgg
    31261 accgtaatgt ccggccggac atcttatccc attccgcagg gagactgcgc cgacccgtga
    31321 tccagttggg gctccacccg cggcgttagg gaaacccctg catgcggcgg agcagcaagg
    31381 cgtttacaat acacgcaaaa ttctaacgat cggcgaccgc tttccctttc catttctccc
    31441 agaaagatct tctcatggtc cgtaaagtca tcccttttca agaacttcgc atgtccgatg
    31501 tggagcaggt gggcggcaag aacgcctccc tcggcgagat gatcagccag ttgcccgcca
    31561 gtgtgcgggt gcccggcggt tttgccacca ccgctgcggc ctatcgcgag ttcctgtccc
    31621 accagggtct ggcagatcgc atcagcgcgg cgctggcgac gctggatgtg gatgatgtgg
    31681 tggcgctggc caaagccggt gcgcagatcc gtcaatggat cgttgatacc ccgttccctg
    31741 ccgcgctgga agccgagatc aaggccgcct acgagcagct gactaccgaa ggtgacggca
    31801 gcttcgccgt gcgttcgtcc gccactgccg aagatctgcc cgatgcgtcc ttcgcgggcc
    31861 agcaggaatc cttcctgaac atccacggct acgagaacat cctgcacgcc atcaaggaag
    31921 tgttcgcgtc tctttacaac gaccgcgcca tcgcctaccg ggtgcacaag ggcttcgacc
    31981 acgcgggcgt ggccctgtcg gccggcgtgc agcgcatggt gcgttcggac atcggcgctt
    32041 ctggcgtgat gttcacgatg gacaccgagt ccggcttcaa cggtgtggtg ttcatcaccg
    32101 cctcctatgg cctgggcgag acggtggtgc agggcgcggt gaatcccgac gagttctacg
    32161 tgcataaggc caccctggcc ctgggcaagc ccgcggtgat ccgccgcaac ctgggctcca
    32221 agctcatcaa gatggtgttt gccgacaaga aggaagccgg caagtccacc cgcacggtcg
    32281 atgtggcgga agccgaacgc aacgtgtatt ccctcaccga cgaggacgta ctggaactcg
    32341 cccgctacgc caccatcatc gagcagcact acggtcgtcc gatggacatc gagtggggca
    32401 agagtggcga cgacggcaag ctctacatcc tgcaggcgcg tccggagacc gtgaagagcc
    32461 agcagtccgg ccacgtgatg gagaagtacc gcctcaagca gtacggcaag tccctcaccc
    32521 acggtcgtgc catcgggcag aagatcggcg cgggcccggt gcgtgtcgtc aaggacgcct
    32581 ccgagatgga gcgggtcaag gccggcgacg tgctggtgac cgacatgacc gacccgaact
    32641 gggagccggt gatgaagcgt gcctccgcta tcgtcaccaa ccgtggcggc cgcacctgtc
    32701 acgcggccat catcgcccgc gagttgggca tccccgccat tgtcggctgc ggcaacgcca
    32761 ccgaggtgct ggacgagaac gacgaagtga ccgtctcctg cgccgaaggc gacaccggct
    32821 acgtgtaccg gggcaagctg gacttcgagg tgatcaccac cgacatgggt aacctgccgg
    32881 acattccggt gaagatcatg atgaacgtgg gcaatccgga gctggccttc gagttcgccc
    32941 agatccccaa cggcggcgtc ggcctggccc gcctggagtt cgtcatcaac aacatgatcg
    33001 gcatccaccc caaggcgatc ctcgagatca accaggtgcc ggccagcctg cgcaacgaga
    33061 tcaaccgccg ctcccgcggc tacgccacgc cgcgccagtt cttcgtcgag aagctggtcg
    33121 aaggcgtcgc caccatcgcc gcagccttct acccgaagcc cgtcatcgtg cgtctgtcgg
    33181 acttcaagtc caacgaatac cgcaagctgc tcggcggcga gatctacgag ccggaggaag
    33241 aaaaccccat gctgggcttc cgcggcgcgt cccgctacat cgcccacagc ttccgcgatt
    33301 gcttcgagct ggaatgcgcg gcgatgaaga aggtgcgcaa cgaactgggc ctcaccaacg
    33361 tgcagctgat ggtgcctttc gtgcgcaacc tggacgaagc ggccggtgtg gtggatctgc
    33421 tggcgcggca tggcctcaag cagggcgaga acgacctcaa gctcgtcatg atgtgcgaga
    33481 tcccctccaa cgcgctgctg gccgatcagt tcctccagta cttcgacggc ttctccatcg
    33541 gctccaacga cctgacccag ctcaccctgg gcctggaccg ggattccggc ctggtggccc
    33601 attccttcga cgagcgcgat ccggcggtca agcagctgct gtccatggcc atccagagcg
    33661 ccaacaagct cggcaagtac gtgggcatct gcggtcaggg cccctcggac cacgccgatt
    33721 ttgctgaatg gctgatggat gaaggcatcc agaccatctc cctgaatccg gacacggtgg
    33781 tggatacctg gctgaagctg gcagcccatt ccaacaaggg ttgaggcgga cgctggacgg
    33841 gggagacccc gtcatgaaag aggccggggt ctcccccggc cttttttgtt tccgttgcgc
    33901 acctgctgca gccgcgccca cctttcatgc gtttcagcgg gccgtggaca aaaaaacggc
    33961 gccccgaggg gcgccggaag gagggagaaa cacaacaggg tcttgcaact ttcgactggc
    34021 gaaagatcct tagaagaaca ggatggcacc cgcggaaatg gtgttgttct tggtttccgt
    34081 ggccgtgttg gtgatcttct cgttgttgaa ttcgccgacg agggtcacga acttgttcag
    34141 gccgtggtac acacccaggg tgtaagcctt gttcttggtt tcgggcgcgc cgtacacact
    34201 ttgagtgccg ccaccggtgt ccttgttctg gccgtagtta atgccgaact tggtcttgtc
    34261 catcttgtag gtgccttgca ggaagtagcc cttggaatcg gtcttgttgc cgttgccgtc
    34321 gctgccgccg aggaattggg cgccgatggt ggacaggccc aggcccttgc cggtgaagcc
    34381 gtaggcgacg gcttcgaagt tgttgtaacc cgccttggcg ccggcttcat aaccggtcgc
    34441 gttatacggt ttcatgccgg cgcaggcggt ggtggaagca ttcgagcagc tggtgttctg
    34501 ggacacgagg ccgccccaca ccttaccggg catagcgccc ttccagtcgt aatccacctt
    34561 ggcctggaag ccgggcgcct tggtttcgtc accggcgaac ttgctcggct ggaagatgcc
    34621 ggcggaggcc tgcaggccgt tgtagttcgg ggtggagtag gtgatctggg cctggaagcc
    34681 ggtgtacatg taaccgtggc cgatcatgcc gaaggtggtg ttgtacggaa tgccggcgtt
    34741 ggaggtgccg ccgacgccca gcagggtcat gtcggacagg atgatgttct gaccgaacag
    34801 gccgatgtca cggccgaact tcaggctgcc ccattcgttg ttgccgaatt ggaaatagac
    34861 gttacgggtg tcgatctggc tgtacgggtt ggtcacgccc gcgccgctgg cgttattgcc
    34921 gatgggcaga cctacgacgg tggagttgtt gttgatgccg ggcgcgaagc tgaagtgggc
    34981 cttgatgtcc tgatcgccgg ccttggaggt gacgacgaag ttgatccagc cgggcagcag
    35041 gccgttggtc agttcactgt tctgggtctt ggtctggacg ccggtgctca ggttggtgac
    35101 cttggattcc cggttcacgt agaagccgtt gatggtgccg ttgatatcga cggtcacatc
    35161 gttctgcgtg aagctgacgg cttgagcgcc gccggcggca gcggccaggc aggcgagggc
    35221 aacggattga gccagaatgg actttttcat atgagatgtc tccgatagat tttttggtgt
    35281 caccagcaaa gcgcggggca agagcggccg ctacttcgtt cctcccaggg tcgtgttttc
    35341 gggaaacacg ccggagcaca cttactatcg cgaggggcat gccaggtcgt acgggaaccc
    35401 gtcccgactt tttgtctgta aattgcaacc gattgtttgt aaaggaatgt tctgctttgg
    35461 gaggcgggcg ctcggggttc gggccgacag gaggctgtgt gcacaaattg aacagtgccc
    35521 catggagcga cgaaaagcgt gaagcagttc atgacacgga atggagcatg ctgtaacgca
    35581 gcatgagcga tcccttcgcg tgcattgccg aggccgacgg cgatgagtcg gggcgggcac
    35641 cggatcaatg acagcggcgt ttgacgtggc gcccaccgat gtggctccgc cgccgagcgc
    35701 gggcttgcgc cggccgcggc tggtagaatc tcgccttcca tactcttggt ccgatccctg
    35761 tgtcttcgat ggatttccgt tcccgtctcg ctgctgcctg gcacagccaa gattccctgt
    35821 tgtgcgtggg gcttgatccc gatccggcac gctttcccgc gcacctcaaa ggtcgtccgg
    35881 acgcagtcct gcgcttctgt ctggaaatcg tggacgccac ggcggatgtc gcctgtgcgt
    35941 tcaagccgca gattgcctat ttcgcagcgg cgcgtgccga agaccagctg gaggcgctca
    36001 tcgcccacat ccatgatcgc catccgcagg taccggtcat tctggatgcg aagcggggtg
    36061 acatcggcgc gaccgcacag caatacgccc gcgaggcttt cgagcgcttt caggctgacg
    36121 cgctcacggt caatccctac atgggtttcg attcggtgga gccgtatctg gcctatcccg
    36181 atcgggggct gatcgtgttg tgccggacct ccaaccccgg cggatccgac cttcagaacc
    36241 tcacgctgtc gaatgggcgc ctgctctatc agcacgtggc ggagctggtg gcggaaaagt
    36301 ggaatctcca tggccagtgc gggctggtgg tgggggccac gttcccggag gaactcgccg
    36361 acgtgcgtca gattgtcggc gatgtgcctt tgctggtgcc cggcatcggc gcccaggggg
    36421 gcgatgtgga ggccacggtg acggctggcc ggacgccttt gggggccggc ctgatgatct
    36481 cctcctcgcg cgccatcctc tatgccggct ccggtgagga tttcgctgct gcggcccgcc
    36541 aggtggcgct tgccacccgc gacgacatca atcgcttccg ccggtgaatt ctggcagagc
    36601 gggccgtgct gcccgttccg ccagcgaagc gcctgccacg accccacgcc cgttccgtta
    36661 gcgcgtcagc cgctgtgctg aggcgtcgtc tcgtcacgtt ttgcagtggc gacccctccc
    36721 cgagaatcgg gtacggacgg gtcgccgcat gtgcaaggcg atcgccatct tgccaactgg
    36781 gtcacgttca ccaatttttc acaaaacatt aaacattgta aaattgtgaa ttaattccgg
    36841 tgaaatccca tgaagttggg aaatacgaga taaatgcgta ttaatttgcg aacgctatcc
    36901 ggcatctgac tgaacatccg tgcaaagccg aacgtcgagg gtgtccggtc ggatcacgcc
    36961 cgtccggcgc gggccgagcg gctcgacgag cacggcggat tgtgtggcgg agcgtccctg
    37021 aacgcctggg gcatccgccc aatcccccaa cagagcagga gaacgagaac agatggctga
    37081 caagaaccgg gtagggtggt actggaagcg cctgcgcgac atgtcggtgt ccgacattgt
    37141 cgggcgtgcg ggcagtacgg ctgcccaggt cgtgggccgg taccggggcg cggtgccggt
    37201 gccgccgttc aggctggatc ggacgggcgc gcgctggctc ctaccggagg tgtcggccga
    37261 gggtgacttc atgggggtgg ctgacgaggt gatgtccggc cattggcggg tgcttgcgct
    37321 ggatcaggcg ccgctggggc tgcctccgga ctggaactgc aacccgctcg atggtgagca
    37381 cttccccctg aacgatgggc gcaccttgtc ccgtcgcgag gacggctcgg gcgacatcaa
    37441 gtatctctgg acgctcaacc gccatcggga gctcgtatcg ttgggccttg cgtggcgaaa
    37501 gactgggtgt cgcgactatc tggatgccat cggcgtgctc atgtcggact ggctcgagaa
    37561 gagtccctac ccccacgggc cgaactggtc gagcgccgtc gaggtggcat tacgtctgat
    37621 gaactgggcg acggtgtggc aactcattgg cggccccaat tcggcgttgt tcgccgaacc
    37681 ggtgggcaag gtcctgcgtt ccgactggct gggttcggtg tggcgccatg ccgagtttat
    37741 ccaggcgcat ctctcgcgcc cggcctcggc ggggagcgat cgtctcggtg aactggcggg
    37801 cttgtatgtg gccggtgcga cttggccgta ctggagccag gcaaaggtct ggcgtgcgac
    37861 cggacgggat gggctggagc aggaggttcc gcgccagatt tccgccgacg gggtggtgct
    37921 ggagcactcc atggccgccc agcgcatgac ctgggacgta ttgtccatcg ctgcactggc
    37981 ggcgcgtgca acgggccggg agttctccca tgccctcggg tgccgcctcg aggccatggc
    38041 ggaatacgtg gcggcggtga tggatactgg gggcaacgta ccccagtttg gcgattcgga
    38101 cgacggcgat gcccttggcc tggggcaggc ggcggggggc tgcgcgtacc gctcgatgct
    38161 ggcgacgggg gccttgttgt tctcgcggcc cgcgttcgcc cgcaaggcgg gcatgcttga
    38221 tccgcggacg cgctggttga tgggcgtcga tgccgcgcgg cagttcgatc tgctgctgtt
    38281 gaaagccttt ccgtccaccc tccgcacggc gtttcccgag ggtggcgtct atgtgctcgg
    38341 tgcgcatctg gatacgccgc tggagtggcg gatcgttgcc gacgcggggg cactcggcgc
    38401 cggtgcgatg gccgagcacg ggcacgccga cgcgctgtcc ttcaccctgt ccgtttcggg
    38461 gcgggaattt ctggtggacc ccggatccgg gacctatcac ggtcagcgag cctggcgcga
    38521 cgccttccgc agcacggccc tgcacaacac gctggtgatc ggcagcgaag atcaggccgt
    38581 gtctggcggt cgtttcctgt ggaagcgcaa gtacgccgtg agcgtcatcc gctgggagag
    38641 cgatgccgac ggggacgtgc tggtggcgga acacgatggc tatcgccgct tgagcggcaa
    38701 gcccgtgcat cgtcggacct ggtcgttcga ccggaagacc gacagtctca gcgtgcggga
    38761 cgaggtcatc gccaccatgc cccagctggt gaccctgtgc tggcattttg gcgaagactg
    38821 tctggtggcc cgcagcacca agggggttgc cgtccataac gggcccgtgc gggttgacat
    38881 ggtcctgccg tccggcgggg acgtgagcat cctgcaggcg tccgagcaac cctttgcggg
    38941 gtggatttcc cgcacttatg accggcgggt gcccgcaacc acggtgacgt ggcggggcac
    39001 gctgtcgccg ggcgatgtcc tcgaaaccga atttcgcaag ggctgagcga ggcgctcagg
    39061 actgatcggg aaagaacaga gcgatcacgg cagagagttc gtcccgcacc cgggcgcgca
    39121 ctgcgcccag catcgaggca tccgcctgca ggatggtgac gattgacggc gacatggtgg
    39181 ccaacacggc gtcctcgtcc acctcgtcgg cgtcgctggc cagcagatca ttggccaggt
    39241 gcaggatggc ggcttcctga gtgtggggac cggcgagcag aggcatgagc tgggcgccga
    39301 ttgcggcgga aaatccggct ggcaggtgcc aggcggaggc cagcgcagcg ccgacttccg
    39361 cgtaatgaca gcccacgacg gactgttcgg cctcgtggat cggaatgtgg tgtctgtgcg
    39421 cgcgctccag cgcggcatct gccatatccg gcgcggcctg gaacatgacc agatggccga
    39481 tgtcggccag cagaccttcc acgaacatcc gctccggatc ccccaggccc agccgatggg
    39541 ctgccgcgcg ggcggccaga ccccgataga cgctgtcctt ccagaagcgg ctcatatgca
    39601 tgcgtgccgg gcggattcca ttgaaggcgc ccacgatggc cgtgccgagc accagatcgt
    39661 gaacctgctg catgccaagc aggttcacgg cccttccaac ggtctcgata cgcccgggaa
    39721 agccgaaaag aacgctgttg accacccgca acacgcgtgc cgtgatcgcc gggtcgctcg
    39781 acaccgcttc ggcgagatcg tgcacggaac tatcgggatc gtcgaggagc gcacggatgc
    39841 ggtggtacac cgcgggcaga ctggtcagtt tttcgatgca ggcgacaagt tcctgggggg
    39901 ttatcatgat ggcgccgggg gtgaggggaa tgccctactt acggctgcgg cggtgcaggg
    39961 ctttagaccc ttgcaggcgc ggagcgggcc ttccgggggg ctctgggcgt gaaatacgac
    40021 agggtttcgt ccgcatcttg acgtgcttcc ggcaattgca cacggcgcgt ccgcttttgc
    40081 tgcggacggc tttcagattg cttgatattc tcgattttga tgattctcgg atgccggaat
    40141 acggtgtccg atgcatgacc gaaccgagga cccgatgaag atattgagct ggaacataca
    40201 gtgggggcgc ggggccgatg gcatggtgac gctggagcgc accgtgtcgg cggtacgtac
    40261 cctgggtgaa tttgacgtca tctgtctgca ggaagtggcc gtggcctttc ctgccctcca
    40321 gggcggcatt ccggtggatg gcgtggccag catcgcggca gcgttcccgg actattcggt
    40381 ccactttgca tccggtgtgg acatgcccga cggaaacggc ggtcgcagcc gatttggcaa
    40441 tctgaccctg tcgcgcctgc ctgtcgggca ggtcttccgc catagcctgc cacgtccgcc
    40501 cgagccgggc atgccgagca tgccgcgggt ctgtctcgaa acggtggtgg aaggcccgca
    40561 gggctgggtg cgggtgatga acactcatct cgaatattat tcggccgtcc agcgcatgac
    40621 ccagatagcc gcgttgcaca cactgcaaca ggacgccgtc agactcgcca acgcgggggg
    40681 tgacacgccg ggccgcaatg cggcgccggg atcgcccttc gaggtgttgc cgcgtccggc
    40741 gtcggctgtg atctgcggag atttcaactg cgagccgggc agtccggagt atttccggat
    40801 gatcgccccc atcggaccgg acgtgtcagg ctgggaagac gcttggcggg cctgctacgg
    40861 ggatattccg cacctcagca cggtagggct caacggcgcg gaatggccgg atcacgcgta
    40921 ctgctgcgat tatttcttcg tgagcgccga tctggtggat cgggtggctg ccgttgcggt
    40981 ggaccaattg acggccgcgt ccgaccatca gcccatcgtc ctgtcgctcg actgatcgcc
    41041 gggcaggcgt gagcaggtaa atgcctcgcg ggccgaaccg tccgccgccc atgtgtgggc
    41101 agcgcgaagg cgctgtcctc ggcgttcatc cggagggcgg ggcaggggat cccctccctg
    41161 ccgcatccgc tcagagcttg ttcagttcct ggacgtagcg ttccgcgttc ttcacgtagc
    41221 cttcggcgct cgcctgaaga cggctgatct cgtgatcggt cagctcgcgg atgatgcgcc
    41281 ctggcgaccc gcagaccagc gaacggtcgg gaatcacctt gccttcgggg atcagggaat
    41341 tggcgccgat caggcagtgc ttgccgatca ccgcgcggtt gaggacgacg gcgttgatgc
    41401 cgatcagcga accttcaccg atcgtgcagc cgtgcagcat gaccttgtgc ccgacggtca
    41461 cattgcgtcc gatggtcagg ggcacaccgt cgtcggtgtg cagcactgac ccgtcctgaa
    41521 tgttggtatt ctcgccgatg gtgatcgggt cgttgtcgcc ccgcaggatc gagccgaacc
    41581 acacattcac gttgcgaccc agtaccacgg atccgatgac ggtggcgttt tccgcgatcc
    41641 agacaccttc tcccagctcc ggctggcgct ctccgagacg gtaaatcggc atgttatttt
    41701 ccttttgtac ggggggctcg gccgtgccgc cgaaaggccg accgaagagc gcgccataat
    41761 atcagggtaa cgaatgtgtg caaggggaaa atcgtgcttt gcaggcgctt tccgtaaggg
    41821 aaagaagggc ttaggccgtc tggatggcat cgggaggcgc ctcttcgggc ccgcctgccg
    41881 accgacgtgt cgaatcgaat tccaacaaca gggaagtctg acccatgagt gatttcgtcc
    41941 ttttcgagcg ccgggacagc atcgtgacgc tgaccctcaa tgcgccggaa acccgcaacg
    42001 cgctgtcgtc tccggccgaa tgggaggcgc tggtcgacgc ctgtgagcgt attcaggctg
    42061 acctttcggt gaaggccgtg atcctcaccg gggcgggaag cgccttctgt gccggcggta
    42121 atgtcaagga tttccgggac aagcgcgggc tggccagggg cggtgggctg gacatccgcg
    42181 agaattaccg tagcgggatc cagcgcatcc cgctggcttt ccatcgcctc gatgtaccga
    42241 ccatcgcggc ggtgaacggc ccggccattg gcgcaggctg cgatctggcg tgcatggctg
    42301 acatccgcat tgcgtccgag tcggcgagct ttgcggagag cttcgtccgc ctcgggatca
    42361 ttcccggcga tgggggcgcc tggctgttgc agcgggtggt gggctactcc aaggcggcgg
    42421 agatgagctt caccggtgat cggctcgacg cggcccaggc gctggaagcg gggctggttt
    42481 cgcgggtcgt ggccccggac gtgctgctcg acgcagcgat ggccctggcc gagcgcatcg
    42541 ccgcccagcc cggccacgcg ctgcgcatga ccaagcgtct gatgcgcgag gctcagacgc
    42601 tgcgcctcga gtcactgctc gaactgtctg ccgcttacca ggcccttgcc cacggttctg
    42661 cggaccatga ggcggccgtg gcggcgtttc tcgaccgtcc caaagggtga tcgcgccatg
    42721 agtgatctgg acgcgctcca gcgccgtgta aaggcctttc gtgacgagcg cgactggggc
    42781 cagttccatg ccctgcgcca cctgatcgtg tccctcaatc tggaggcggg ggaactgctg
    42841 gagctgatcc agtggcgacc cgacgcggag atcgatgcac tgccgtccga tccggtcggt
    42901 cgtgaggcac tggcggacga atgcgcggat gtgctgctct atctgctgct catcgccgac
    42961 cgggcaggaa tcgacctggg cgtggcggcg ctggccaagc tcgagaaaaa cgccgccaag
    43021 taccccgtgg aacgcttccg cggctcgcgc cgcaagtaca acgcgccgga gtgacgtaca
    43081 cggcgcagcg acaggcgggc cgaggtgata gcatcgcgcc cctgaccgtc ccgcgccgac
    43141 cggcgccacg cctgccatgc tgagctaccg ccacgccttt cacgccggca accacgccga
    43201 cgtgctcaag cacttcgtcc tcgtccagtt gctggaatac ttcaaccgca aggacaagcc
    43261 ctatctgtac atcgacaccc acgcgggggc cggctgctac gggctggaag aggcctttgc
    43321 gcgcaagaac gccgagttcg aggacggcgt ggcccggctg ctggcgcgca aggacgtgcc
    43381 ggccgagctg aagagctatc tgcgcatcgt gcgggatctc aatccgccgg gccgcctgcg
    43441 cctctatccg ggctcgccgt gctgcgcggc gccgctgctg cgggagcagg accgcatgcg
    43501 cctgttcgaa ctgcatccgg cagacaaccg cctgctgcac cagaccttcg ccgaggcggg
    43561 cagcaaggtg atgatcaagg aggaggacgg cttcaccggc atcaatgcct tcctgccgcc
    43621 accgtcgcgg cgggcactgg tgttgatcga tccgccctac gaggtgaaga ccgattaccg
    43681 taccgtgctg acggcgctca aggagtgtct gcggcgtttt gcgacgggta cgtatgtggt
    43741 gtggtacccg cgccttacca caatgcacgc ccgcgagctg cccgagaagc tcaagcgcct
    43801 gcccctcgac agctggctgc acgtgagcct ggacgtgaag aagccatccg aggacggctt
    43861 cggaatgcac ggcagcgggc tcttcgtgat caatccgccg tggaccctgc cggagacgct
    43921 gaagacggta ctgccgtggc tcaaggatgt gctggcccag gatgacggtg cggacttcgt
    43981 cctggagtcg cagatcccgt gaacgcaccg gggcccggca cggcagaacc gtgacgggcc
    44041 ccggaacggg caccgctgcc gcaccgcccc ggcgggggcg cggcggtggg ccggcttaca
    44101 gcaggtgcat cttcttggcg gtctcggtga gctcgtcgcg gaacttggga tgcgcaatgc
    44161 tgatcagcgc ccgggtacgt tccttggcgg tcttgccgcg cagctgggcg acaccgaact
    44221 cggtcacgac gtagttgatg tcgttcttgc tggtggacac gtgggtgccc ggcgtgaggg
    44281 tcggcacgat gcgcgagatg gtgtcgttct tggccgtgga gggcagcacg atgaaggact
    44341 tgccgccctt ggagcggttg gccgcacgga cgaaatcgga ctggccgccg gtgccggagt
    44401 agggaatcgg gcccaggctc tcggaaccgc actggccgag gaagtcgatc tccagcgtgg
    44461 cgttgatgga caccaggttg tcgttgaggc cggccaggta gggatcgttg gtgaagtcca
    44521 ccggatgcat ctcgaaggcc gggttgcggt cgatccagtt gtagagcttc ttggagccga
    44581 gggcaaaggt agcgaccatc ttgccgggca ggtagttctt gcggcggttg gtgacggcgc
    44641 ccgactcgat tagcgtcagg atgccgtcgc cgatcatttc ggtgtgaatg cccaggtcgt
    44701 gcttgtgggt gagctgcatc accacggcgt cggggatgcc tccatagccg atctgcaggg
    44761 tggagccgtc ttcgatcatg tcggccacgt acttgccgat ggcttcctgc accgggccga
    44821 tggcgggcag accgacctcg agcaccggtt cattgttctc gaccagggcg gtgacctggg
    44881 agatgtgcac ctggttgttg ccgaaggcgt aggggacgtt ctcgttgacc tcgaggatca
    44941 ccgcgcgggc cttgctgatg gccgccatcg tgtagtcggc ggcgaggccg agggagaagt
    45001 aaccgtgtgc gtccatcggc gaggcctgcg tgaagacgac ttcggcaggc atcaggccgc
    45061 gttcgatgag ctgcggcatt tcggagaaat agctggggcg gaagtccacc acatcttcgt
    45121 ggaagcccgg ccgggtggcg ccgctgaaga agtaggcgac gtgacgcacg tgctgggcgg
    45181 tggtcgggtc caggtaaccc atcttgcgta ccgcgaggat ctgggaaacc ttcacgtcgt
    45241 ggaagtcgcg acggtgctcc gagagggcgt tgagcagggc gggcggttcg gccacggcgg
    45301 tcggcacgat gatggtgtcg ccattgtgga cgtgacgaat ggcctccgcg gcgctcatgc
    45361 gcttggcggc gtattgttcc tgcacagtca tggtgttgtt ctcccttggt cgtgaatttt
    45421 ttcgagatta aaccacgtct tttagaggtg atttgtactc gattgtacga tccgcagggg
    45481 gctccggcgc ttggtctgga tcaaggaaag cccccgtggg acaagggatt cacggggcga
    45541 aggagcggtc gcggccaagg caggtgcggt gacgggcatc gaccctttcg gcgaaccagg
    45601 cggtggtcag cttgcgctgg atcttggggc tcttcaggtc gatctcgggc aggatttcgc
    45661 ggggctggcg acgaccgtcg agactgtcgg ccagggtaaa gatgcggata tagagcgggg
    45721 tttgggcaaa ctgggaggtc ttctcgaggc gcaggtcgcg ggcgatctcc gccggactca
    45781 ggccgaggcg gccgccgagc gtgctcagcg ccgagtaggt ggcgctgggc tcggacgagg
    45841 gcgctccgtc gcgataacgc aggaggtcgc cgtccggcgt cagcgcttgg ccggagaggc
    45901 gcgccacggc gttttggaag gctgcattgc ggctgctgta gcgacccgcg ttgaagtcgg
    45961 cgaagcggta caggaggcgg ctgtagggcg ctgggtaatc gagcaggatg gccaccccga
    46021 aatacacgcc gccgcggcgc gcgaagactt cgtcgcgcac gctgccacga cggggatacg
    46081 ggtagggctt ctcgcggatc tggtcctggg cgaaggcgat gctcacctgc atcgggccgc
    46141 cggtgcgcac ggggttgtaa tcggcgaaga gcgtcttgcc ctggggaaac tcggagatca
    46201 tctcctcgaa cagggcgctc atctgttttt cggtgcgtag ggcgtcgatg cgtgctcggt
    46261 agctgcggcc gtccggggag ggggcgagga gtgcggcgtc gagcatcagc ctgggaaggt
    46321 gatagcgctg gcgacgtgct tcgagttccc gccagacgat cttgggcagg ccaggcaccg
    46381 cggggtcggc ctggaagctg gactcctgtt cgatgacggc aaccgcggcg cagatcgtgt
    46441 ccggcgtgag ggacagatgc agggccgtga aggcggtgtg gaggtcggtc gcccagccct
    46501 cccggtcact gacgcccggc ggcaggaagc gggcgatcag ggcgcgaccg tcctgggcgc
    46561 tcggtatcgg gggcggtgcg ggtggcgtgg gggcggtgcc aattggagca ggcgcgggcg
    46621 agacagggcg tgggcgatct atgtcggccg gtgcccgcgg agtgggcgaa cccggcgccg
    46681 gcggcagggt gggcgaaggt cgggattcca gcgggccggt ggtggcgcat ccggccagtg
    46741 tggcggccaa ggtccaggcg gcgagaagcc tgcgttgctt caaggcttgt cggcagcctg
    46801 ttgctcggct tgggcgacgc ggccgaggac gcgggtgtaa tagccggcgt aggccagcgc
    46861 gaggaaggcc atcaggcccg tcgccaggag ggcgccttcg gcgggggctc ccgccaggac
    46921 gttgctgacg aaggaatagc cgagcacgcc ggcggaagcc atgccgccaa tgatgcggat
    46981 gagggcgaag gtcttttgct gtttgagggt cggaggcgcc atcgagggag atctgcagat
    47041 tcggacgggc gcgaggatcg gatgattccc cgcgcgcgtc aagcggaccg tcacatgatc
    47101 tgaagccctg cccagtaagc cagtgcggcg atcagggccg agcacgggat ggtgaggacc
    47161 caggcccaca cgatggtgcc ggcgaggccc cagcgcacgg cggcggttcg gcgtaccgag
    47221 cccacgccga cgatggcgcc ggtgatggtg tgggtggtgg atacggggat gcccaggctg
    47281 gacgccatga acagggtcag cgcaccgccg gtctcggcgc agaagccgcc caccggtttg
    47341 agcttggtga ggcgctggcc catggtcttg acgatgcgcc agccgccgaa cagggtgccg
    47401 aggccaatgg cgaggtaaca caggacaacc acccacatgg gcaccggatc accctgggcg
    47461 gtgacgccgg cggcgatgag gagcatccag atgatgccca cggtcttctg ggcgtcgttg
    47521 ccgccgtgtc ccaggctgta cagcgccgcg gagacgagtt gcaggcggcg gaaccatttg
    47581 tccacccggc gctggggctt gtgccggcag atccaggcga cgatgaccag catcagcgag
    47641 ccgagcaggt agcccaccag gggtgagacg aggatgaatg cgacggtctt ccagatgccc
    47701 gccgagatca gcggcgcagt gccggccttg gccatggcgg cgccgaccag tccgccgatg
    47761 agggcgtggg acgagctcga cgggatgccg tagtaccagg tgatgacgtt ccaggcgatg
    47821 gcgccgatga gggcgccgaa gatcaggaaa tgatcgaccg cggcgggatc gacgatgccc
    47881 tttccgacgg tgctggccac cttgaggtgg aacacgaaga gggcgacgaa gttgaagaag
    47941 gcggcccagg cgacggcctg gtggggcttg agcacgccgg tggagacgac cgtcgcgatg
    48001 gcgttggcgg cgtcgtggaa gccgttcatg aaatcaaaga gcagcgccac gaccaccagc
    48061 ccggtgacca cccagaggct gatatgcagg gattccatga cggactcagg cgttttcgag
    48121 cacgatgcct tcgacgatct tggcgacgtc ctcgcagcgg tcggtgatgg attcgagctg
    48181 ctcgtagatg gccttgagct tcatgacctg gagggcgtcc cgctcatccc ggaacaggcg
    48241 cgacatggcg gcgcgcatga tgcggtcggc atcggtctcg agacggtcga tttcctggca
    48301 ggtctgcagg atggcgctgg cgttgtccat gttggagatg agcccgacga tgatcttgac
    48361 gcgggtgcag caggacaggc tgatgttggc gagctggaca gcctccggcg tgctgcgctg
    48421 gaggtggtag agggtggtgc actcggccac gtcctggacg gtgtcgagga tgtcgtccat
    48481 cgcattgatg agactgtgga tctcgtcccg gtcgaggggc gtgatgaagc tcttgtggag
    48541 ctgggcaatg gtgtcgtggg tgatgcggtc ggcgctctgt tcgatgccct cgatggcaga
    48601 gatgcgacgg tcggcgctgg agaggtcttc catcagcgcg acgagttcct gggcgccgag
    48661 cacgatctgc gccgcgtggg cattgaaata ctcgaaaaat tgcccttctt ggggcaggag
    48721 acgtccaaac atgggctgtt tattgcaacg tgatgactga aatgttgcag tttaacagag
    48781 ctggataggc tcgggccggg tcagcaagcg actgtggggg cgaagcaacg cgcactcgcg
    48841 acactttgac aggtctggac gcggcgcgcg gggtcgtccg atgctggcgc ggatgattcg
    48901 gccccggctg ggccggggca gggtggcccg gtcagtcttc aggcgcgggg tttgcgtggc
    48961 ttgaagccgc cagggccgtc cttgcggggc ggctttccgg ctggacgccc gccgcgcggg
    49021 cgcgcggatt cctcggcgct ggccgggcgc aggttgaggg cctgctggcg aacgcgggcg
    49081 cggcgcaggg tctgcaccac gtcgggcggt agattggcgg gcagttcgac ggtgctgtag
    49141 tcttcgtaga ggttgatctg gccgatgtag cggctgtcga tgccgccttc gttggcgatt
    49201 gcgccgacga tgtcgcgggg gctggccagg tgttcgcgac ccacgtcgat gcgatagcgc
    49261 atcaggcgcc cttcggcgta gtcacggcga cgttcgagga tctcgccgcg gttttcgcgg
    49321 gccggacgtt cgcgctgggc gctgccggca aactgggcga tgtcgggctg gccgttgtcg
    49381 ggcatctgca gcgggcgttc cttctgggcc aggaaggcga gggcggcggc gatctcgtgg
    49441 atttcggcgt cgtgtgcgtc ctcgatgccg gcgatgacgt cgaggaagaa gtccagttcc
    49501 tcggacgcca gaacttcggc cacctgctgg cggaaggcgg cgacgcgctt gtcggcgacg
    49561 gcctcgcggc tgggcagctt gagcgcttcg ataggctgac gggtggcgcg ttcgatctgg
    49621 cgcaggaggt ggttttcccg cggggcgacg aagaggatgg cattgccggt gcgcccggcg
    49681 cggccagtgc ggccgatgcg atggacgtag gcttcggtgt cgtaggggat gtcgtaattg
    49741 atgacgtggc tgatgcgcgg cacatcgatg ccgcgggcgg ccacgtcggt ggcgacgacg
    49801 atgtcgaggg tttcggcctt gagctgctcg atgacccgct cgcgcagctg ctgggtcatg
    49861 tcgccgttga ggcaggcagc ggcgtaaccg cgggcttcaa gcttttcggc gagctccacg
    49921 gtggcggtct tggtgcgcac gaagacgatg gcggcgtcga agtcgtcttc cacttcgagg
    49981 atgcgggtca gggcgtcgag cttgtgggcg ccagcgatct gcaggtagcg ctggtggatg
    50041 gtggagacgg ttgccgtggc ggccttgatc ttcacctcgc ggggttcgcg cagatagcgg
    50101 tgggcgaccc gccggatggc gtccggcatc gtggcggaaa acagggcggt ctgccgctcg
    50161 gcgggggtgt gttcgaggat ccactccacg tcgtcgataa agcccatgcg cagcatttcg
    50221 tcggcttcgt cgaggatcaa ggtggcgagg ccgtccaggt tgaggctctt gcgctcgatg
    50281 tggtccatga tgcggcccgg cgtgccgacg atgacgtggg cgccgcgctg gagctggcgc
    50341 aactgcacga ccatgctctg gccgccgtaa aggggcagca cgtggaagcc ggccatccgg
    50401 ctggcgtagc gctggaaggc ctcggccacc tggatggcca gctcgcgggt gggcgtgagg
    50461 accagcacct ggggttcgcg gcgggcgagg tccacccggt cgagggccgg cagggcgaag
    50521 gcggcggtct tgccggtgcc ggtctgggct tcgccgagga tgtcctggcc tgcgaggagg
    50581 tggggaatgc aggcggcctg gatgggcgaa ggggtttcgt acccgatctc ggccagagcg
    50641 gcgaggatcg ccggttgcag gtcgagctgg tcgaagcgtt cgatggcgtc agtcatggac
    50701 ggcgggaggc gcgggccggg tggaggggga agcagtcccg ggcccagggc ggaggcagct
    50761 tcagggaggc gggcattcta ccggaatcgg cccgcccgtg ccgcgggcta ggggcgtttc
    50821 acggtgccgg cgtcctgtcc gaacaggacc tggcgggcgg catcatccac cacgggggcg
    50881 gcgtggatgc gctcggccag ggcgtaggcg cgcacggtgg cgggtcgtgc ccggatggcg
    50941 tcgaaccagc gggcgaggtg gggaaagtgc gcgaggtcct gttgctggcg gtcgtggggc
    51001 acgatccacg ggtagcaggc catatcggcg atcgaatagt ctgcgcccgc gatgaagtcc
    51061 cggtcggcca gacggcggtc gagcacggcg tagaggcggg cggtctcctt gacgtagcgt
    51121 tcgatggcgt aggggatgcg ttcgggggcg tactgcacga agtggtggtt ctggcccgcc
    51181 atgggaccga gtccgcccat ctgccagaac agccattgca gggtgtcgag gcgcacgcgc
    51241 agttcggtgg agagaaagcg cccggtcttt tcggccaggt aaaggaggat ggcaccagat
    51301 tcaaagaccg ccacgggcgc gccgccatcg gccggggcgt ggtcgacgat ggccggaata
    51361 cggttgttgg gggcgatgcg caggaagtct ggctggaact gctcgccgcg cccgatgttc
    51421 accggcacga tgcggtaggg caggccggtt tcctccagga acagggtgat cttgtggccg
    51481 ttgggggtgg tccagtagtg caggtcgatc atggcgggtg gcctcaggcg caggggtggc
    51541 gctcggcggt cagcacccag gcggggtcca gttcgcggat tggcacggcc gtgtcggcgg
    51601 ggatgcggcc gctgagttgc aggcgcaggt agcgcaggca gcagacgcgc aggaaggagg
    51661 cgaagttggt gctgtcgtcg aggcggccgc tctgcaggag ttcgtcgtag agtcggctgg
    51721 tcagctgggg caccgtgagg ccgtcacggg caccgatttc ttcaagcacc tgccagaaca
    51781 ggttttcaag gcgcaggctg gtggccacgc catgcaggcg cagggagcgg ctgcgggtgg
    51841 cgtagaggtc ggggtcggcg ttgatgaaga tctggcacat ggtggtcgcg catccaggca
    51901 cacgggcagg cccccgtctg gccattgtag gcggtccgga ggcgccgcgc ctcagaggct
    51961 gatgcgtgtg cccagtacgg cgaggaactg ggcgatccac gccgggtggg cgggccaggc
    52021 gggtgccgtg accagattac cgtcggtgac ggcctggtcg atggggatgt cggcataggt
    52081 gccgcccgcc agttccactt cagcgcggca ggcgggatag gcggagcagg tgcggccttc
    52141 cagcacgcgg gcgccggcga ggagttgggc accatggcag atggcggcga cgggcttgcc
    52201 ggcctcgaag aagtggcgca cggcggcgat caccgtcggg taggtgcgca ggtactccgg
    52261 accgcgacca ccgggaatca gcagggcgtc gtagtcttcg gggcgcagct cggcgaaggt
    52321 ggcgttgagg gcgaaggcgt ggccgcgctt ctcggtgtag gtctggtcgc cttcgaagtc
    52381 gtggatggca gtggcgatgg tgtcgccggc cttcttgccc gggcacgcgg cgtccacgcg
    52441 atggccgacc gccagcaggg cctgaaacgg caccatggtt tcgtagtctt cgcagaagtc
    52501 gccgcagatc atcaggattt tcttggtcat gaaagtctcc gtgggggtgt gggaggttaa
    52561 agtgtggcga gagggtcggg cgtgtgggtg gtagcgggtt accaccggag cgggcgtgcg
    52621 gcgctcattc gggcgccggt tacggcgcag acggcaggat tgacgtaagg agcgtgtgtc
    52681 gtggcgtatg tgatcgaacc gcccagaccg gtggtggtgc cagtgttggg ggacgggctg
    52741 tttccagtgc gtcgggtgta ttgcgtggga cgcaattacg cggcccacgc ccgcgaaatg
    52801 ggggcggatc cgacgcgtga ggcgccgttc ttcttctgca aaccggccga cgcggtggtt
    52861 tccgtgggac cggaagggat gctggagcta ccctatcccg tggccacggc caacctgcat
    52921 tacgaggccg aattggtggt ggccatcggc ctggggggcg cggacattcc tgtcgacgag
    52981 gcgctggccc acgtgtgggg gtatggcgtc gggctcgaca tgacgcggcg ggatctgcag
    53041 acggcgctca aggacaaggg gcggccgtgg gagctgggca aggccttcga ccgctcggcg
    53101 cccctgtcga cgctggtgcc ggccggggcg atcggtcacc cgcagcgggc ggcgatctgg
    53161 ctggagcgga acggcgagtc acgtcagcgg gccgacattg ccgacatgat ctggtcggtg
    53221 gcggaaatcg tcgcgcacct gtcgcgttat ttccggctgg agccgggtga cctggtcttc
    53281 accggcacac cggacggcgt agggcccgtg gtggccggcg acgtggtgga ggccggggtg
    53341 gagggtgtcg ggcggctgcg ggtacgttac gtgtgaggcg tggtgtcggg gttcagtccc
    53401 gcagggcgtc ggcccagcag ttgggcgttt cgtagaggcg gatgcgctca aggcgcaggt
    53461 ggttgccgta cacgtcgcgg tagagtggat caagaatgcg aaaggcctcg gcagccagat
    53521 tctcggcggt ggggacgacg tcgaggatga ccgtcttgtg tccgggcatg gactggagga
    53581 agcccaccac ggccgtgtcg tggcgatagg ccaggaaggc gtggtcccac acatcgacga
    53641 catgttcctt ggcgatggcc ttgacgtcgg caaagtccat gaccatgccg ttgatcggcg
    53701 caccgtcggc ccggacgacg tcgcccgaca gggtgatctc gagggcatag cggtggccgt
    53761 ggaggtggcg gcactggctg gcgtggtcgg gaatgcggtg gcctgcatcg aattccaggc
    53821 ggcgcgtgat tcgcatggga acggcggacg ggaaaaaagg tctcgtatta tacgcagctc
    53881 gtttcccgca ccggatcttc catgaccgct tcctcgcaga tgctcaccgt cgccgatcac
    53941 aaccgcagct tcagtgcgct gacctacgtc tatcccgtgg tgtcacggcg ggcacggggc
    54001 gtgtcggtgg ggatcaacct caatcccaac aatgcgtgca actggcattg cgcgtattgc
    54061 caggtgccag ggctggttcg gggggcggcc ccgtccattg atatcgagct gctggcggcg
    54121 gaattgcagg gtttcctgac agaactgcag cagggccaat tcatgaagga tcatgtcccg
    54181 gaagagatga gggtgatccg cgacattgcc ttttcgggaa atggggagcc gacaagctgc
    54241 aaggcatttg ccgacatcat ggaacgcgtt gtcggaatcc ggaacgcgtg cggtctgagg
    54301 aatgtgccga tccgggtcat cacgaatggc agtctcgcgg atcgcccttc cgttcaggcg
    54361 gccttcagga cgctcgccga agcggggggc gaggtgtggt tcaaggtcga tgccggtaca
    54421 cgggaagaga ttctgaagat caacggcgtg gatattgatc cggccgcgca cgcgcgtcgt
    54481 ctggcattga cggcttcgat ttgcccaaca tgggtacaga cgtgtgtttt ccggtgggat
    54541 ggcgaattgc cgacacaaga attttcagat tcatatgtcg catttctgca aaaagccgga
    54601 ctcgaggggc ttaaaggcat cctgctttat ggcgtcgtac ggcaatcgca ccagcctgaa
    54661 gccatccacc ttcagcccct gagtgccgaa gaactcgagc acattgccgt cgccctccgg
    54721 aaaaccgggc tgaccgtgaa tgtcagcccg taagcggcgc gtccgctcag tgaggaacac
    54781 gttccttgcg cttggcgcgg gcttcctttt tgagcgtctt gaacagctcg ggattcgtgg
    54841 ccttaagata ttcaacaact tccacgtcat ccttcttgat cttgttgatg tccacctgct
    54901 cgatatgcac gaggcggagc agggcctgga cggggcgggg aatgttgcga ccactctcgt
    54961 agcgggagcc gccactttgc gtcacgccga ttttggacca gaactgggac tggttcatgc
    55021 caagcttgcg acggatttcg cgcacgtcca ccttctcatt cgtcttcatg ttgccatcct
    55081 tcttagataa gtcactctgt cagtttgagc agcgcatcag gagttttgct gatataactt
    55141 tcgttatatg tcagaaagta tagattacta cgcggaggac atacaacgcc ccattctgtt
    55201 cgattagaga aatctgaccg ttttttttgt gacaggatcc acacccttca tgacgggatt
    55261 tttgccgatc tcggttcaga cgggaagcag gggcatgacg catgaggaag ctgcacgaac
    55321 tgctctgatc cgctaagatt gcatgttttc cacacccttc actcttagaa gtcaagatgg
    55381 cgctcatagt tcaaaagtat ggcggcacct ccgtcggaaa tcccgagcgg atcaagaacg
    55441 ttgccaagcg ggtcgcgaag tttcatgccc aggggcacca gaccgtcgtg gtcctctccg
    55501 cgatgagcgg ggagacgaat cggctgatcg ccctggccaa ggaaatcagc ggcagtccga
    55561 gcccccgtga gctcgatgtg gtcgtgtcca ccggcgagca ggtcaccatc ggcctgctct
    55621 gcatggcgct ggaagaagtg ggcgttccgg cgcgcagcta caccggcggt caggtgcgca
    55681 tcctcaccga cagttccttc accaaggccc gcatcctcga catcgacggc gacaacatgc
    55741 gtcgtgatct cgatgccggt cgcgtggtgg tggtggcggg cttccagggc gtcgatgcgg
    55801 aaggcaacat caccaccctc gggcgaggcg gctccgatac cacaggcgtg gccctggcgg
    55861 cggcgctcaa ggcggacgag tgccagatct atacggacgt ggatggggtt tacaccaccg
    55921 atccgcgcat cgtgccggag gcccgcaagc tggacaccat caccttcgag gaaatgctcg
    55981 agatggccag cctgggctcc aaggtgctcc agatccgttc ggtggaattc gccggcaagt
    56041 acaaagtcaa gcttcgggtc ctgtccagct tccaggagga aggcgaaggc accctgatta
    56101 ccgttgagga agacaagaac atggaacagc cgatcatctc cggcattgcc ttcaaccgcg
    56161 acgaagccaa gctcaccgtg ctcggcgtcc ctgacaagcc cggcattgcc taccagatcc
    56221 tcgggcccgt tgcggatgcc aacgtggatg tggacatgat catccagaat gtcggtcacg
    56281 acggcaccac ggatttctcc ttcaccgtca atcgcagtga ttacgacaag acggtcaaga
    56341 tccttcagga cgtccaggcc cacgttggcg cccgcgagat caagggtgat cgcaccatgg
    56401 ccaaggtctc catcgttggc gtcggcatgc gctcccaccc gggcgtcgca tcccgcatgt
    56461 tccgcaccct ggctgaagaa ggcatcaaca tccagatgat ctccacctcc gagatcaaga
    56521 tctccgtcgc catcgacgag aagtacctgg aactggccgt gcgggtgctg catcgtgcat
    56581 tcggtctgga tcaggccgct gcgtgatttg accgacacgc ctggcgtatc tataatgccg
    56641 ggctcgtgtg gagagttggc cgagaggtcg aaggcactcc cctgctaagg gagcatacgg
    56701 gccaaaactc gtatcgaggg ttcgaatccc tcactctccg ccacactgac ctgggccccg
    56761 aaaggggcct ttgtcatttc tgcgccaccg tccgctccgg ggcaggcaaa aggggcgtct
    56821 gcggcgcggg cagcgggcgg tacaggcgcg ctgtgcgcct tccatcgcca tctccagcac
    56881 caagccccgg ctggtgaatc ttcgcctggt ggggtgggtg atccggcttg ggtccgggcc
    56941 ggcgcgggag cgcggcgccc ggcacatcgt ggaggccggc gtaccgcgca gtgcgcagta
    57001 gtcctgtgcc acccgcagct caaaccggct acgcaaatgg ggcaggaacc gggcaccgga
    57061 tgccgtcgcg gggcgcgtgg cgccctcttg tgcggcgatg gtgaaatccg cctgccggtt
    57121 cgaggcgcgc cagccgatgc aatcggggtg ggggtgtcgc tcgcggggcg cggatgggtg
    57181 gacgctccgc cacatgtccg tcggttccgg ccggaacaca tgagcggtga gaccgacgcg
    57241 gccgtgtcgg caggaaggtg gagggtcagc gcgtgtcctg ctcaatggcc cacacggcgg
    57301 cttctaggcg cgaacggaac ttgagtttcc tgagcagact cttgatatgg accttgacgg
    57361 taccttcggc gatgccgagt tcccggccga tgagcttgtt cgagagtccg gtggcgagac
    57421 actggaggat ggcgcgctcc cgttccgtga gatgagcgag gctgcgttcg gctgattgcg
    57481 cctcttcgcg cagggaacgg gcgagcaggc cggtcaggct gtcgtcgatg acgatccggc
    57541 cggcgagggc ttccgacatc cgtgcgagga gatcttccgg ctccgtgtcc ttcagcagat
    57601 agccatcggc gccggcgcgg atggcggcga ccaggtcctc ggctgcatcg gatacggtca
    57661 ggatgatgat gcgtgcgccc aggtcggcgt cccgcaaggc gcgcagggtg tcgatgccgc
    57721 tcatgccctt catgttcaga tcgagcagga tcagatccgg atcggccgtc tgggcaagcg
    57781 tgagtccgtc ttctccgctg gaggcttcgc ccacgaggcg caggcggtcg gacagggcga
    57841 tgagctggca tacccccttg cggaacaggg gatgatcgtc gatcacgatg acagtgctgg
    57901 gtgcttcggt ggcggtcatg gggaaattcc acggggtgag ggtggggcgg gtggttcggc
    57961 cggaaaacac agctccacgc gggtgccacg atccgtacgg gccgtcacgg tgagcgcgcc
    58021 atcgagactg cgggcgcggt cgtgcatgat ggcaatgccg aagcgtctcg gcggtgccgg
    58081 ctgttcgccg atgccgacgc cgtcgtcgtc gatgcacacg cgcaggtcgc cggcgtcgag
    58141 gcgctcgagc acgatgtcca cggtgtgggc gcgggcgtgc cgctcgatgt tggagagggc
    58201 ttcgcggatg atctgcagga cgtggatctc gcggccggcg ggcagttcca ggccggcaat
    58261 gcgattgctc agatggatcg cgaggccgcc ccgttggctg aattcccgga cgctgtcgtc
    58321 gatggctgcg gtgaggccgc gtccgtcgat acgcaggcgg aaggtggtga ggagttcgcg
    58381 caactgtcgg taggcctcgt tgaggccgtc gcgcagctcg cgcaccacat cgcccaccgg
    58441 gtcggagacg gactggcggg cgagaaggtt ctgcaggcgg gtcacctgga tcttgaggta
    58501 ggacagggac tgggccaggg agtcgtggag ttcccgtgcg atcaccgccc gctcgtccag
    58561 gagcgcgagg cggtgacgtt cctcatggcg gcgggcgttg gcgagggctg cgcccacatg
    58621 gcgtcccacg gtttcgagca gcggtgtctg ccactcggcc ggcggggcac tgtcagggag
    58681 ctgcaggaac aggcggccca ggctgcggac gccgtctgag acggggacgg tgatgagtgc
    58741 gccgtcgcct gcgtaggaga gggcaggtag cacctgcaac gcggccgggg cggccgatgg
    58801 cggcccgtcc agaggcagac gggcgacgag gtcggtgtcc atgtcggtgg ccagcacgac
    58861 gagcgggcgc ccgtcaccgg gatcggcgca cagggcgcca gcccgaacgc cggccagtcg
    58921 ttcgacctcg ttcagcacct tcaacaggct gtcctgtgtg aggtcggcct cggacagcgc
    58981 gcgggtcgtg cgatagagca gttcgagcga ctggttgctg cgggccagtt cccgggtctt
    59041 ttcctcgacc cgcgtctcca gctgggagta actgcgggac agatcctcca ccatgtagtt
    59101 gaaggcttcg ccaagctggc cgagttcgtc cggcggccga ggggcggcgc gaaccgaaaa
    59161 gtcgccgcgt cgtacgacgc gggcgcaggt cagcaattca ccgaggggcg cgcgtacctg
    59221 gcggttgatc tgatggacca ccacaccgcc cagtacgagc agcaggaaga gggagatgcc
    59281 ctggacgagg cgcagggtct gcaggcggga ttccagattg tgctcgatca gcgcgaccag
    59341 atgatcgatg cgcgccacca gatcggcggt caggcgattg cgcgcgtccc ggtcaggctc
    59401 tggggcggct tcagcatacg ggcgaaaggt ctgggcccag aacagtccga cgtcgtcgta
    59461 ggcgagtcgg atggacgcct cggcggatgc cggcagggcg gtcgtgagcg cctgggcggc
    59521 atagcgttca tcgaatccgc tgagcgcccg ctcgagcagc gcgccgtgtt cgccgtcggc
    59581 gagggtggcg gcgatgacat aggactgcat gcgcagggag ccggcgaggt tgatcgcacg
    59641 ggcctggcct gaggccagct cagcgacggt catggaggcc aggatggaca cgacggccag
    59701 cgcgccgatg gccgcgatca gggcgccgag gcgcgcgacg acggaaagtc gtcgaggcgc
    59761 acgagcggtg acaaaggcgg ctgcgtcagg gggcctcacg ccgggtctcc gctgcagggc
    59821 gcatggcctg gcttccatcg gaaactttgg gataggccag tgctcgccat gtgcgtacgg
    59881 agatcgctgg gctgcgtgcc ggtgttagta aaaatagtgt tacacaacaa catgttgatg
    59941 aacattttcg ggtgaatttc gggtatcact ttcggggtag tcgatgccaa tgtcttcatg
    60001 gccgcctctg acttaaatca aatcgaggcg aaggcgcttg cctagactgc gcgcatggtc
    60061 gggctgctgg cgtggccgga ccgcaagcca aaaattacat ccacgcggga gaaaccatga
    60121 agaccagtcc tgtcgagttg tcggccgtcc tgccgggcat tcgtctgttt gcggggctgg
    60181 atccccgggc ggtggcggaa ctgtcgcggg ccggtcatgt ggccacgctg cccaagggtc
    60241 gcgggatcta cgccaccggc gagctgccgc gcgccctata ttacttgctg tcggggcagg
    60301 tgaaagtggc ggtaacgtct gcggaggggg gcgagaaggt catcgacatc gtccatgcgc
    60361 cccaggcttt cggggtggcc gaactgttcg gacaggcgcc ccacgtgtca tcggcggaga
    60421 cggtgggggc gtccgtggtg ctggtgattg gtcgcgaggg cgtcatgcgg gccatcgaat
    60481 acgacgcgcg gctttccatg cgcctgctgc agaccgtcgc cgaccgccag acggccatcg
    60541 aacgggacat cgcggcggac tgcttccagt ccggctgcga gaaggtggtg gactatctgc
    60601 tgcgcgcggt ggctccggcc gccggttggg gcgacagcgt cagcctcgat ctggagattc
    60661 cgaagtatct gctcgcggcc cgcctgggct ttacgccgga gactctgtca cggatctttc
    60721 gcgaactgtc cgatgaaggg ctgatccgcg tacatggacg tcgggtggag ctgctcggac
    60781 gcttgctgcg tcggcacggc gtggtaccgg cgcaggtacg cccgccgcgc agcccggagc
    60841 gcggtcgatc cggtcaggcg gtggcctggg cctgatcccg cagccgggcg acggcttccc
    60901 gcaccagcgg gtcgagtccg gccgcgccgc cggcgcaggc gatcagcccc gcgcgcatgc
    60961 ggggatccca gaagcgcatg agatgctcgg ccacgtcgat ggcggcgcgg gccggctcgg
    61021 cgttggcggc gacagccgtg gcgatctggt tggccatgtt gacaagcttg gcctcattca
    61081 tgggggatgc ctcccgtggc ggtggcctgc aggccgcgct ggcgatggtg gcgcagggcg
    61141 aagcgctgtt gccagtcgga gggctgggcg cacagggtga cctgcacggc ggtgaccttg
    61201 tattcggggc agttggtggc ccagtcggag ttgtccgtgg tgatcacgtt ggcgcccgat
    61261 tcagggaagt ggaaggtggt atagaccacg ccgggctgta cccgctcggt gagggtggcg
    61321 cgcagcaccg tctcgccggc gcgactggcg atgccgaccc agtcgccgtc gcggatgccc
    61381 cggtcttccg cgtccgtggg atggatctcg aggcggtcct cgccgtgcca ggccacgttg
    61441 ggcgtgcgcc gtgtctgggc gcccacgttg tactggctga ggatgcgacc ggtggtgagg
    61501 agcagcggga agcggcgggt gacccgttcg gaggtgggga cgtagggcgt gagcatgaag
    61561 cggccctggc cgcggatgaa gcggtccacg tgcatcgtcg gcgtgccctc cggcgcatcg
    61621 gcgttgcagg gccactgcac gctgccgcgg cgctccagca gctcgtaggt gacgccgctg
    61681 aaactggggg tgaggcgggc gatctcgtcc atgatgtccg acgggtggtc gtagggcatc
    61741 ggatgtccca gcgcggccgc gagggccagg gtggcttccc agtcggcctt gccggagagg
    61801 ggcggcatca cccgccgtac acgggagatg cgccgctcgg cattggtgaa ggtgccgtcc
    61861 ttttccagga agctggcgcc gggcaggaag acgtgggcga acttggcggt ctcgttgagg
    61921 aagaggtcct gcaccacgac gcattccatc tgggccagag cggcctccac gtgctgggtg
    61981 ttggggtcgg actggacgat gtcctcgccc tggatgtaga tcccgcggaa gctgccgtcg
    62041 agcgccgctt cgagcatgtt ggggatgcgc aggccgggtt cggggtcgag acgcacgccc
    62101 caggcggcct cgaagctgcc ccgcacggtg gcgtcggaaa cgtgccggta gccgggcagc
    62161 tcgtggggaa aggagcccat gtcgcagctg ccctgcacgt tgttctggcc acgcagggga
    62221 ttcacgccga cgccggggcg gcccacgttg ccggtggcca tggccaggtt ggcgatgccc
    62281 atgaccatcg tggagccctg gctgtgttcg gtgacaccga ggccgtagta aatggcgccg
    62341 ttgccggcgg tggcatacag gcgggcggct gcgcggatgg cttcggcggg cacgccggtg
    62401 acggcctcca cggcttccgg ggcgtgctcg gggcgggcga cgaagtcgcg ccaggcctgc
    62461 caggaggcca ggtcgcaacg ggcctgcacg taggcttcgt cggcgagacc ttcggtgacc
    62521 acggtgtggg cgagggcgtt gatcatcgcc acgttggtgc cgggcaacag ttgcaggtgg
    62581 tgggtggctt ccacatgggg cgagcgcacc aggtcgatgg cgcgtgggtc cacaacgatc
    62641 agccgggcgc cctggcgcag gcgccgtttg aggagggcgc cgaacaccgg gtggccgtcg
    62701 gtggggttgg cgccgatcac caccaccacg tcggccttca gaacagaggc gaagtcctgg
    62761 gtgccggcgg actctcccag cgtctgcttg aggccgtagc cggtggggga atgacagacg
    62821 cgggcgcagg tgtccacgtt gttgttgccg aaggccgtgc gcaccagctt ctggacgagg
    62881 taggtctcct cgttggtgca gcgggacgag gtgatgccgc cgatggcgcc gcgaccgtgc
    62941 tcggcctgga tgcgccggaa ttcggcggcg gcgtgggtga cggcctcttc ccagctcacc
    63001 ttttgccagg gggcgtcgat gctcgggcgg atcatcggcg tggtgacgcg gtccgggtgg
    63061 gtggcgtaac cccacgcgaa gcgacccttg acgcaggcat ggccctggtt ggcaccgccg
    63121 tcgcgggttg gctccatgcg caccacggag ttgcccttga cctccgcatc gaaggaacac
    63181 cccacgccgc agtaggcgca ggtggtggtc acgcggcgtt cgccctggcc gtgttcgatg
    63241 acggactttt ccatcagcgt ggcggtgggg cagacactca cacaggcgcc gcaggacacg
    63301 cactcggagg ccatgaagga ctcgtcctgg ccggcgcgga cccgtgaatc gaaaccgcgg
    63361 ccgacgatgc tcagggcaaa gctgccctgg atctcctcgc aggcgcgcac gcagcggttg
    63421 cagacgatgc acttggacgg gtcgtagcgg aaataagggt tcgaatcgtc ggcccgatcg
    63481 cccaggtggt tggcaccgtc caggccgtat cgcaccgcgc gcaggcccac cgcgccggcc
    63541 atgtcctgga gttcgcagcg gccgttggcg gcgcaggtga ggcagtccag cggatggtcc
    63601 gagatcgtca gttccatcac attgcggcgc agctcggcga ggcgcggtgt gtgggtgcgc
    63661 acgaccatgt ccggcgccac cggtgtggtg catgaggccg gatagccctt gcgcccgtcg
    63721 atctcgacca ggcacaggcg acaggcgcca aaggcttcca ggcggtcggt ggcgcacagt
    63781 ttggggatcg cgcagccggc cgtgtgggcg gcgtgcatca cgctggtgcc ggcgggcacg
    63841 cagactggtt ggccgtcgat gaggaggcgg acggtggctt cgccgtcgcg ggccggggtg
    63901 ccggcatcgg gggggcaggg cagggacatg gcgggcgtgc ctcaggcagc ggtcggcgcg
    63961 aggccgaagg cgtcgggaaa atgcgtcagg agggactgga cagggagcgg cgccatgccc
    64021 cccagggcgc actgggagcc gtggatcatg gtgtcgcaga ggctgtccag caaggcccgg
    64081 tcgcggggcc ggtcggtacc tgcgaggagg cggtcgatga gttccacgcc gcgcacgctg
    64141 ccgatgcggc agggcgtgca tttgccgcag gattcgaggg cgcagaattc cagcgcgtag
    64201 cgggccaggc ggcccaggtc ggcgcggtcg tcgaaggcga cgatgccgcc gtggccgatc
    64261 atcgcgccca tggcggcgta ggtctcgtag tcgaggggca cgtcgaagcg ggactctgga
    64321 atgtaggcgc ccagcgggcc gcccacctgc acggcgcgaa tcggccgtcc gctggcggag
    64381 ccgccgccgt agtcgaggag gagttcgcgc agggtgatgc cgaaggcctt ttccacgagc
    64441 cccggatgac gcaggttgcc ggcgagctgg aagggcaggg tgccgtggga gcggcccatg
    64501 ccgtaatccc ggtagaaggc ggcgccgcgg gcgaggatga ggggcaccgc ggcgaagctc
    64561 atcacgttgt tgatgacggt ggggcgcccg aacaggccgc tcagcgccgg caagggcggc
    64621 ttggctcgca cgatgccgcg gcggccttcg aggctttcca ggagggccgt ctcctcgcca
    64681 cagatgtagg ccccggcccc cagtcggagc tcgatgtcga aggcttgccc gctaccggcc
    64741 acatcgtcgc cgagccagcc ggcgctgcgc gcggtggcca gggcggcttc gagcacgcgg
    64801 aaggcatggg gatattcggc ccgcaggtag atgtagccgc ggcgggcgcc gaccgcgagg
    64861 ccagcgatgg tcatgccctc gacgaggcag aaggggtcgc cttccatcag catgcggtcg
    64921 gagaaggtgc cggagtcgcc ttcgtcggca ttgcacacca cgtatttgtc gccgtcgccg
    64981 gccgtctgca cggtggccca cttgatgccc gtgggaaagg ccgcgccacc ccggccgcgc
    65041 aggccggagt cggtgacttc tcggacgacg gcggcatcac ccagggcaag ggcccgacgc
    65101 agaccgtcga agccgccgtg gttctggtag tccgcaaggg atcggggatc gatcacgccg
    65161 acacggcgga aggtgaggcg ttcctggccg gcgaggaaag ggatgtgctc ggtgaggcca
    65221 tgacccagtc ggtgcatgcc cccggtcagg aaaccggcgt cgaagaggcc gggcacgtcc
    65281 tccacgctca ccgggccgta ggcaaggcgc ccggcaggcg tgtccacctc caccagaggt
    65341 tccagccagt acaggccacg ggatccgttg cggacgagct cgaccgtcac gccgcgggag
    65401 gcggcttcgc ggaccagggc acgggcgacg tcgtcggcgc cgacggagag ggcgccggag
    65461 tcggcgggca cgaagaggcg gacgttcatg gggcgggctc cgatgtgcgc aggcgggcga
    65521 tcagcgcctc caggcgggcg acgtccatgc gaccgaggag ttcgtcaccg atgcggacgg
    65581 caggggagca ggcgcagttg cccaggcaga agaccggctc cagcgtcacc gcgccgtcgg
    65641 cgctggtgcc atgccagccg gtgccgagca gactgcgggc atgcgcctcc agggcttcac
    65701 cccccctggc ctgacaggat tcggcacgac agatctgcag cacgtggcgg ccgggccggg
    65761 tacggcggaa gtggggatag aagctcagca cgccatggac ctcggcgcgg ctcaggttga
    65821 gggcggtggc caacatcggc acggcgtcgg ggggcaccca gccgatccgg tcctggaggg
    65881 cgtgaaacag gggaagcagg gcgtcgggcc ggtgcccgtg gcgtgccagg gcgtcggtga
    65941 cggcggcgtg gagaacgtca ttcatgggct gtggacggtg aaattctgcg cgtgatctta
    66001 gacgcactgg ggcgcgccgt gaatctccct gaacatgacg ccgatccgac ggcaacccca
    66061 cgtgtccgca gcgggcctgg aagatgacat gcgggttgtc cgggctatgt tgaaggggta
    66121 acattcggcc tttgcataga aggatgtcgg gaacatgctt ggcgcagcgg attcagctcg
    66181 ggacgaagtc cattcctcgg ccatgcgacg ctgggggcgg atcttcggcg tggttgcggg
    66241 cggcgcgctg ggcgtgctgg cggttccagt aatcgtgtcg cagacatcgc tgagcggaga
    66301 ggccctgacg gaaaccgcaa gaggggtggg gtggggcgca ctcgtcctgg cggcaggtct
    66361 ggcgggacga tgggcacagg gacgtcgtgc gcatcgggtg gcccagacgg aaggcgccgc
    66421 tgcggtggcg acggcgccat cgggaagtca gccccggcgc gcacgcgcgt ggtcggtgga
    66481 cgtgctgcaa cagctggatc gtgcgcgctt ccaggcgctg tgctgcgcgt tcttccggga
    66541 aagcggggtg gccgccacga cccacgcggg ggatgaggac agcagcgcgg gggacatccg
    66601 tctgcaccag gatccacggc gccccggtgc ggtgaccgcc gtcgtgcggt gtgtgccgtg
    66661 gggaccgacc atcgacgtgt gggcggtccg ggcgctggcc gaaacccggc gacgggaaag
    66721 cgccgaccgg gcgttcttca tggcccccgg cggcttcgcc ccggacgcgc tggcctttgc
    66781 cgcgcagggg cggatgaccc tgttcgacag cagattgatg ctggcattga tccggcggct
    66841 tcccgaagac agccagcagc gtctgctgga cctcgcggcg acgggggact ggacggctcc
    66901 ggcgtgtccg gaatgcgggg agcggatggt cgtccgcaac gtgatccgcc gtccgttctg
    66961 ggggtgctcg cgttttcccc agtgtcgcgg caccctgccg atgaaccgca aggcgggtct
    67021 ccagggcgac gacgggccgc tcgcgatggc cggataggcg tggtcccgcg ggctggcggc
    67081 ggtgccttga ccggcgccgg gtgtcgcggc cggtagcggt ccatcaccga tccgggccgg
    67141 gtccggattt cagggcacga tcagcagctt gccggtggcc tggcggtcag ccagcagcgt
    67201 gagggcctgg cctgcctcct caatgccata acgtccgtga atggggggct ggaagatgcc
    67261 ccgggcgatg ccgtcgagga tcgctgccac ctgtcgccgg cattcggccg gttcccggcg
    67321 caggaactcg tcccagaaga cgcccgccat ctcgcatccc ttgaggagca gcaggttggc
    67381 cggcacccgc gggatctcac ctgccgcaaa gccgatgatc aggacgcggc cacgccaggc
    67441 ggtggcacgt acggcggctt cggtataacg gtcgcccacc acatccacga cgatgtcggc
    67501 gccccgtccg ccggtggcgg ccttgacggc gtccttgaga tcatttgcat ggtagtcgat
    67561 gcccgtgtgc gcaccggcgg cggcacacag gtccagcttt tcccgactgc tggcggcggc
    67621 gatgacggta cacccggcgg cgcgggcgat ctggatggct gccagtccga cgccgccggc
    67681 cgcgccgagc acgagcaccg tctcgcccgg ctgcatgcgg ccccgatcgt gcagggcgaa
    67741 ccaggccgtg gcgtaggtga cgagggctgc cgcagcgctt tcgaaactga ctgcgtcggg
    67801 caacggaaac acctgcgcgg cccgggcccg ggccttctcg gcgtagccac cgaaggagtt
    67861 gaaggcgagc acccgttgtc caactgtgaa ttcatggacc tgcgcgccta cggcacacac
    67921 ggtgccggca aactcgccgc cgggtgagaa cggcaggggc gggcggatct ggtagcgacc
    67981 ctggacgatc agggtgtcgt agtaattgac accggcagcg gcgacgtcga tgacgatctc
    68041 ggtatcggcg ggctgcgggt cgccgatctg tcccggtccg tgctggtcga gggggccgaa
    68101 agcctggcag agataggcgc gcatctgtgg catggctccg tggtggcggg aaatcagtgg
    68161 ggatcgtcga gcaggcgggc cacgaagccg gcgatggcct cgacgacggc atccgcctga
    68221 tccttgtggg gagagtggcg gcagtccgcc agcttcacca gatccacatc cggcgcgagg
    68281 cgggcgatcc ggtcgatctg atccatcgtg gcgtactcat cgtcctcgcc ctggatagcc
    68341 atcacggggc agggaatggc ggacacgaag gcttcaatgt tccagtcgcg gaaatccggg
    68401 tgcagccaga tgtcgctcca gcccgcgaac acgccatcca cgtcggcatg aaagcgggcg
    68461 aggcgcgcgc gcaggtccgt ggtccgatag gcgtcgacgg tccggcggat gccctccagt
    68521 gccacatcct ccacgtaaag gtggggggcc atcaccacca cgccacggac gggcgccggg
    68581 gtgccgcccg cgtggatgag ggcgatggag ccgccgtcac tgtgaccgac gaggatcggt
    68641 gcgcgaagct gcagggcgtc gaggacggcc ggcagaatct ggagcgcctc cacatgcatg
    68701 tagtccggtg tgcgcggtgc agtcagcggc gaggagcggc cgtagccgtg acgcgaatag
    68761 acgatggcct cgcagccggt ggcgtcggcc acgcgttggg ggaagtcgcg ccacatggcg
    68821 atggagccga gcccttcgtg caggaagaca agggctgggg cctccgggcg tggatgggcg
    68881 gaagggcggc ggacgacctc gagggtgtgg ccgcgcaggg cgagggtgtg catggcgggt
    68941 gcgctgttct cgtggttttt ggggacagtg tgcaccggaa cggggctcag gggaacgccg
    69001 tatcggcctc acggggacgt gcatcagggt gccgcgtcgg gacgtgccgc cgggaggctt
    69061 tgattttcgc gcaatgtgtg tataatgccg gttcttcagc gcgcccgtag ctcagttgga
    69121 tagagtactt ggctacgaac caaggggtcg tgggttcgaa tcctgccggg cgcgccaatt
    69181 tacttcaaga gtcgcgacaa gcggctcttg cagcttcagg gggcggttct gtcagcgtcc
    69241 tggagttggg agttgcaagc gcccgtagct cagttggata gagtacttgg ctacgaacca
    69301 aggggtcgtg ggttcgaatc ctgccgggcg cgccagacaa aaagggattg cattcgtgca
    69361 atcccttttt tcatttcgcg ccgctgcgct gcagtgtcag ccctcgatcg gccgtgttcc
    69421 ggtgacggag accggccgga tgcgccctcc cttcagtagg cgttgggatc cttgcggatc
    69481 agccggacgg tgcgcaggat gatccgcagg tccagggcga gggaccagcg gcgcaggtac
    69541 tccaggtcga aatccacacg ctgcttcatc ttctcgaggg tttcggtctc tccgcggcag
    69601 ccattgacct gggcccagcc cgtgatgccc ggcttgacct tgtggcggat catgtagccc
    69661 ttgatgagct tgcggtacat ctcgttgtgg gcgacggcat ggggccgcgg gcccacgatg
    69721 ctcatgcgcc cctgcagcac gttgatgaac tggggcagct cgtccagaga ggttttccgc
    69781 aggaaggcgc cgaggggcgt cacacgcgca tcgttctggc gggcctgggc gatggtctcg
    69841 ccattctcgc agaccgtcat ggagcggaac ttgtagacca ggatgcgttc cccgtcgagc
    69901 ccgtagcggt attgccggaa cagcgccggc cccggcgacg agcacttcac ggccgccgcg
    69961 atcaccagca tcagcggggc gatcaggatc aggatgcagg ccgccatcag aaaatcaaaa
    70021 gactgtttca gcacgccgtt gaggcccgtg aagggggttt cgcagaccgc caccacgggc
    70081 atgccgccca catgatccac ccgcgcctgg atgaggtccg tgacgaacag gtcggggacg
    70141 aaatagaccg agacggtcgt gtcgcgcaga tcgtccagca gacggacgat gcgtggctgg
    70201 gtggtcatgg gcagggcgat gtagatcacg tccacaccct gctggcgcac gtaatccgcc
    70261 agatcggcga tgcgtccaag cagccgcagg cgcggactgg ccagacgctc ggcatgacga
    70321 tcgtcgaaaa atccgagcag ctgcttgcct tcgcggcgtg acgcgcccat gcggnnnnnn
    70381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    70441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagttca aagattttgc aaatcgcgta
    70501 ttagttcgct tgttttcgcg attccctcat tcaagatgcg cgtgtttcga acttgagggg
    70561 caacttgttt tgcggcgcat cggcttacaa ctggggggag cagtgctcat gctgggcgtg
    70621 ggaagccccg tgctcgccct cgacgcgtcc gcattgcggg tgtcgaccct gctcggcgaa
    70681 gccagccgtc aggccgcccc cctgttcgag agcgacgagc tcgatcctcc cgccctgacg
    70741 ctcaccggga ccctggggcc ggaagcggcc ggattgccgg agcggggagg tgagccgctg
    70801 gatctgccgg tcggcaagag cgccgggacg cggatttccg tcgccgagaa cctgacctac
    70861 gacagcaacg ttttccgcct gcgggattcc gcggcggcgg tagccgccgg actggcccac
    70921 ctgggctcct ggcagagtca gaccaagctg ggtctgggcc tggacaagtc ctacgccggc
    70981 cagcagctgc tcctcgacta cgacgcgacc atggcccgct acagccactt cggcttcctc
    71041 gatcacaccg tccagggtgg catcggccgc ctgcgctgga aggccgggca ggatctgaag
    71101 ggcgagctgg tctccgaata ccgggaggcg ctggacgact acgcctacta ccgcagcccg
    71161 ttgcgcagca tctcgacgac gcgccagatg ggcggcaatg cgttttacac cctggtgggc
    71221 ggctggcagg tcggcgcgca ggccgggcgc ggcagccgcc gctacaagga tggcacgcgt
    71281 cccaccaacg agatcgattt tgccggcgtg gacggtgtgc tgcgctacgt accggagcgc
    71341 ggcggcgcgg tggccctcac gcggcgccgg gcacggggcg aataccccaa cctgccggcg
    71401 acggcgggct cccttgcgga gacgcgcttc aatcaggacg agaccttcct cgaagtgtcg
    71461 ctgccggtgc gcagcggctc gcgctacctg atgcgcgtgg ggcgtgtcag ccggaattac
    71521 gaacgctatc cccagagcaa tttttcggga cgcaacggcc tgatcggcgc cgaatggcag
    71581 gtcacgccgc gcacccagct gaacggctcg ctccgctatg acgtggagcc ggcccaggat
    71641 tttgtgtcga gttacgtggc caccaagggt gcgcgcctgg gcgcggtgtg ggagtactcg
    71701 ccgaagacac gcatcgagag tcgctacgag tggcgcaacg cccagtaccg cggcgatccc
    71761 ggattcggcg tggtggccgc aagccgccgc gaggacacgg cgacgctggt gggggtgacg
    71821 gcgctccacg cgatgtcggc ccggacccgc tggaccgcga gcctgagccg ggaacggcgg
    71881 gactcgaacc agcaggaatg gtcattcacg gattggattg tggcagggag tctgcagtgg
    71941 gtattttgaa gcagggtagg gagggctggc agcgctggct gctgtgcgtg gtcgcaatgc
    72001 tggccgccgc gctgacgggc agcctcgcgc tggcgcaggg cccggctccg gccgcgcagg
    72061 agtatcgact cggtcccggt gacggggtca agatttcggt cttccagaac cccgatctgg
    72121 ggctcgagac ccgggtgtcc gagaccggcg tcatcagcta tccgctggtg ggcagcgtgc
    72181 aggtgggcgg gctcaccgtc ggcgaggcgg agaaggccat cgccgcgggt ctgcgcagcg
    72241 gcggctatgt gctccagccc caggtgaccc tggtggtgca gcaggtgcgc ggcgcccagg
    72301 tggcggtgct cgggcaggtg gcgcgccccg gccgctatcc gctggaaagc accgcgatga
    72361 aggtcaccga cgccctggcc ctggccggcg gcaccacgcc ccagggcgcc gagaaggtga
    72421 tcctcaccgg cctgcgcaac ggccgccggg tgcgtctgga ggtggacgtg gccgacctct
    72481 tcgtcacatg gacgcccgag aaggacgtgc cggtgcacgc cggcgacacg ctctacgtgc
    72541 agcgggcgcc ccagttctac gtgtatggcg aggtcctcaa ggccggcgcc taccgcgtcg
    72601 aacgcaacat gacggtgctg caggccctgt ccctgggcgg cggcctcaac ccccgcggca
    72661 ccgagcgggg catccgcatc caccgccgcg gcgacgacgg cagcgtgcgc gtcatcaacc
    72721 cccaactcaa cgatccggtg caggcggacg acgtgatcgt cgtgcgcgaa gccctgttct
    72781 gacggaaccc cgatcatggg aatcacccaa ctcctggcag tactcaaggc gcgtcgcacg
    72841 ctgctcttcg gcgtgtgggg cggcgtcgtg ctcctggccc tgctggtcag tctgatcctg
    72901 ccgcgccagt acgccgccac cgccgaggtg gtggtggaat cacgcggctc cgaccccctc
    72961 gcgccgcctc agggcgtggg gctcaccacg ccgggcttcc tcgtcaccca ggccgacatc
    73021 atcgccagcg aacgggtcgg ggcccgcgcg gtggcggccc tcaagctgat ggatcagccc
    73081 cggtgggccg agcgctggca gaaggcctcc ggcggccagg gcgatccggc gcgctggatc
    73141 gccggcaagc tcaagaagac cgtcaaggtc agcccgtccc acgaaagcaa cgtgctcacc
    73201 atcaccgtga ccgcggacga tcccgcgttt gccgcgagct acgccaacgc gctcgccaag
    73261 gcctacctgg aaaccgacct ggaactgaag atcgagccgg cccgcgaata cgccacctgg
    73321 ttcgatgagc acaccaaggt gctgcgcgcc aacctggagc aggcccagtc gcgcctgtcc
    73381 gccttccagc gggagcacgg cctggtgggc gacgagaagc tcgacctgga aagcgcccgc
    73441 ctcggcgagc tgtccaccca gttcaccgcg gttcaggccc agagcaccga ggccaccgag
    73501 cgccagcgcc agggcggcgg ggccaacgag gtggccagca accccctgat ccaggccctc
    73561 aaggtggatc tggcacgcca ggaggccaag ctcacggagc tgtccggcta cctcggcgaa
    73621 aaccaccccc agtaccagcg cacgaagagc gaggtggagt ccctgcgtgc ccgcctgcgg
    73681 caggagaccg gcaacgtggc ggcgatggtg ggcaccaatt cccgcatcag ccaggcccgc
    73741 gaaggcgacc tgaaggggcg cctcgacgcc cagaagcagc gcgtgctggc cctcaagcag
    73801 cagcgcgacg aaatgatgct gctccagcgc gaggtggaca gcgcccagaa ggcctacgac
    73861 ctggtgatgg cgcggctgac ccagaccagc ctggagagca aggcccgtca gggcaacgtg
    73921 gcgctgctgt cgtcggcggt ggcgcccacc gaggcgagca gcccgaaggt cctcctcaac
    73981 gtgggtctgg ccctcgtcat cggcgccctg ctgggcctgg cggtgaccat cgtgcgcgaa
    74041 ctcttcgacc ggcgggtgcg cgccgccggc gatctggcgg acgccctggg cgtgccgctg
    74101 gtggtcgagg tgaaggctgc gccggccgca gccgccggag cccggccctt ctggccgcgt
    74161 ctgcgccggg cttgaggaca gatcatggat aaagcgaagt tttccggatt cggcaacgtg
    74221 gccccgctgg agcgcgcgcg ggatgccacg cccagtctgg gcgagttgct ggtgagcgcc
    74281 gggcgcctgt cggcggacgc cgccagccgg gtcgccacgc gccagcgtga gcgggggatt
    74341 ccctttggcc aggcggcggt ggagctcggc ctggtgagcg aggcggacgt gcgcgcggcc
    74401 ctcgcccgcc agttcgatta ccccgtgctg gcggccggcg atccttcggt ggcgccggag
    74461 ctggtggccg ccttcacgcc cgacgcgccg caggtggagg ccctgcgcaa gctgcgcagc
    74521 cagctcgtcc tgcgtcgcgc cggcaatccc cactgcaaga ccgtggccgt cgtcggcgcg
    74581 ggccgcggcg aaggacgcag ctggctcgtg gcgaacctgg ccatcacctt ctcccagctg
    74641 ggcgaacgca ccctggtgat ggacgccgac ctgcgcaccc cgcggcagca ggcgctgttc
    74701 cggctgccgc ccgcgggcgg gctggcggcg catctggcgg aacggggcga cagccagcca
    74761 ccggtcgttc atccacgctt tccggggctg gcgatccagc cggccggcaa cgtgccgccc
    74821 aatccccagg agttgctggc ccgctccggc ttcgccgccc atgtgacagc cgcgcgcgac
    74881 ttctacgaca tggtgctggt ggacaccccg gccgacgagg tcggcgccga cgcccagctg
    74941 gtggccgccg cctgcggctt tgccgtcgtg gtggcgcgcc ggcacatcac cgcccacaag
    75001 cgggcgggcg ccctggcggc gagcctgcgc gatgcgggcg tgacgatcct cggcggcgtc
    75061 ctgctggagt tctgatgcgc ttcgccctgc ctgccttcga tgtcgccgag ctgacggcct
    75121 tccacctgga aaaccgggtg gcgcgggccc tcacgtgggc gatgttcgtc ggactgtggg
    75181 ccaagctggc cgtgtcggtg ctgctggtgc agccggtgat cgacgcggaa gcagattcgt
    75241 tctacagccg cgcggtcgac gccatgctgc tgtgcgcgat cttcgtcggc ctggccactt
    75301 acgcggacga gctgctgcgc cgcgcgcccc tggtgctggc cggggtcccg ctggtgctgg
    75361 cctcgttcat gacgaccgaa gtcctcaccc tcgacggctt tgccctgacg accttcgaat
    75421 acctcaagat cgtcacgccg cttctcatgt cggcggtgct gctgcgcctc ttcgagcggg
    75481 agcccgccct tgtcgtgcgc ctgatgcagc tcaccctggc catcgtgtgg ctgggggtga
    75541 tcggcgcgct gctcttcctg ccgccgtccc tcaaccgtgg cggggagccg ttctggcccg
    75601 tgtatttcgc gtccctgcac accagctcct acctcgtcgt ggtgtgtgcc gggctgtccc
    75661 tctatctgta tgaggccgac cacttcggaa agcgcctgtt catcgcgcag atcctgttct
    75721 ccttcgtcct cgtgttccac ggctggggtg tgcgcacctc catggtggcg gcggtggcct
    75781 tcggcctcgt ctatttcttc cagcattccc gcaaggcggc gctggtggtc ttcctgacga
    75841 tcgtcgccgt cctgaccctg gcctacctgc cgctgctgtt cggcgtcgtg cacctgccgt
    75901 cctgggacga gatggtggaa ctgtcctccg ggcgcatgtc catgtggctg cagaaggccg
    75961 acatcctggc caacagcacc ctgctggagt ggttgttcgg gcacggggag gggtccgact
    76021 ggatcgtctc cgagatctgg tggtggagcg ccaaggattc ccacaacgac ctgatccgtc
    76081 tgctcaatca gcagggcctg ctcggcctgg gcggcgtgct gtggctgctg tgggtgtggg
    76141 cgcggctgtt cccggccggg gtggcggcgc ccttgctggc cgccctgctg gcgtccacgg
    76201 ccttcagcaa cggcatcatg ttccggcccc aggcgggcct gctcatgccc ctggccctgg
    76261 tggcggcggc cgatgcgtgg cagcgccgtc aggcggggag ggcggcatga gccgggatct
    76321 caccgccctc gtgcgccgcg tcgaggtgct ggccgtgacg gctgcgcccg gcggacagcg
    76381 cctgctgcat ttcatgctgg tgcagatggc ctgcggcacg gaagccctcg gccgcttcgc
    76441 caatgacctg gccatcgcca tgatgctggg cctgttctcc gcggtggggt ggtcggccct
    76501 ggtgctggcg cgggtgccgg tggcgccggc cgggcgctcg gcgccggtga tgctgggcct
    76561 cgcccgcggc gtggccgtgt tcaccgcgct cgcctgcctg ccgctgcccc tcctggcgtg
    76621 gggcggctgg gtcttcgaac cgggctggac ggcgctgctg ctggccggct ggacggccta
    76681 cacggttgcc cgccactacg gcctcgccct gggtgcctac cgggtgctgc tggtcgtgga
    76741 gaccctggtc gtcgtgctcc tcgcggcagg cctgctgatc ttcggccggg cccatcccgt
    76801 catggcctgg gccgcctttg ccgtgccgac ggtgctgctc ggcgccctcg cggtcctcgt
    76861 gctgatccgg cgggcacgcg accagggcgg ggcggctccg ctgccggccg gcagtgcccg
    76921 cacggccgcc gagcaggccg ccctcaactt cgtgagcggc ggcatgtcca tgatcctcgt
    76981 gccgtgcacc gtgcaggtgg ccggcgaagc ctacggcggc ctggtggcgc tgatcggcag
    77041 cgtgaccagc gtgctgctgc tcttcccgcg ggccctcagc ttcaatgccc tgccggccct
    77101 ggcccgtgtg gtgcggcagc agggcgcagc cgacgtgcgg gccctgctcg atgccctgcg
    77161 gcgcaccctg ttccgtgtga acagcggcct caccctggtc gccgtggcgg cctgggggct
    77221 catggccggc gtgggcgaac gtcccgcatt cgcgctggcg ggggccaccg ccatcttcct
    77281 gctgcagctg gccagcttct acgtggggca gctcgtgctg cccgaggcca actacctgca
    77341 gaccgtcgag gagacccgcc tgccgctggt ggtgaacctg gcctccctgg ggctcttcgt
    77401 ggtggcctgc gggggcgtga tgctcctcgt gccaaagggc gtcgacgcgg tgctggggct
    77461 cttcgcggtc ctgctggggg cgaccttcgc gcgcaacctg ctgttgcgcc gggcggtgct
    77521 gcaccgggcg ccggaggtgt tcgcatgaac gtcctgcgtc tgtccatctt tctgccggac
    77581 ggcccggtgc cgggctgggt ggtgcgcctg ctcgacgtgt gtggcgcgga gcccggcgtg
    77641 atccttgtcg gcgtcctcta tgacgcgcag cccatggctg cggccgactg ggcggagcag
    77701 gtggatcgcc tgctcttcga ccggggcgcc tcgtccctcg atccggtgtc cctcgaactg
    77761 cccggcctgc tgcccgtgtg gcgtgcggag gccggtgtgg cgccggcgcg ctggctcgcc
    77821 gagcgggcct gcgacgtgct gctggacctg cgaccgcggc ccaccccgat cccccagctg
    77881 gccgccgagc actggatgct gagcatcgcc ggccgacctg ccacggacgc gggggcaggc
    77941 gcccagcccg tcgtcggagg cgacgccacc acctgcgtgg ccctcgagcg ggtgggcggc
    78001 ggtgcgatcc tcgatacctc cgtgggggcc acctgcgaat tttccatccg ccgcaaccgg
    78061 cgccgcgccc tcgccaaggg catcggcatg gtgcgccgcg cgctgcgccg gatgcgcacc
    78121 caggtgcccc cgccgcccca gcgcgacacg ctgcccgcag cggccgtgca gc
//
LOCUS       DAOQPA010000003        74973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_112_length_74973_cov_18.618089, whole genome shotgun sequence.
ACCESSION   DAOQPA010000003 DAOQPA010000000
VERSION     DAOQPA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 74973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74973
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112_length_74973_cov_18.618089"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1658)
                     /locus_tag="PKX01_00845"
     CDS             complement(261..1658)
                     /locus_tag="PKX01_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HMZ74694.1"
                     /translation="MFTRRLLAALVPAVLATSAHAGVTLIGTGTLSGPDMSGLTGALE
                     SGASASTLGGIGSGVAWAGGDTFLFTPDRGPNATAWNSAVDDTTSYVSRFQTVNLGLS
                     SAVFTSGSVTTLTPTLTDTTLLSSATPLNYGSAPALNSSGVNYFTGRSDAFGAGLSSN
                     PNNARLDPESIRVAQNGKSVFVSDEYGPYIYQFDRATGERIGVITLPSKFAVSNLSSQ
                     GATEISGNTSGRVANKGMEGLAITPDGKTLVGIMQSPLIQDGGTDGSTVRIVTIDIAT
                     GKVTHEYAYKLDNIGTVAKPKYPTVSEIVAINDHEFLVDERDGKGLGDGSNAAVKKLY
                     KIDLSGAADVSALSGAANLAPKAVGKTLFLDVVAALTSVGYTTATIPAKLEGVAFGPD
                     VLVSGVVEHTLIVANDNDFLSTVGGIANPNRVFVFGVTDDTLPSYQAQRIPEPTTLAL
                     AALGLMGCMVGRRKG"
     gene            complement(1942..3855)
                     /locus_tag="PKX01_00850"
     CDS             complement(1942..3855)
                     /locus_tag="PKX01_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ74695.1"
                     /translation="MTQPQSAVQAPLAVSFADLALSEPIQRAIADTGYTVPTPIQVQA
                     IPQVLAGGDLMAAAQTGTGKTAGFTLPILHRLSEAHAHNVQTGRPRCLILTPTRELAA
                     QVEESVQTYGKHLPLTSMVMFGGVAINPQITKLKKRVDILVATPGRLLDHCTQKTVDL
                     SGVEILVLDEADRMLDMGFIRDIRKILAMLPKQRQNLLFSATFSDEIKALADGLLHNP
                     GFVEVARRNTASELVRQSVHLCPQKQKRELLAFLIQKHQWFQVLVFTRTKHGANKLAE
                     YLGKHGIPSAAIHGNKSQSARTRALADFKDAKLQVLVATDIAARGLDIDQLPQVVNFE
                     LPNVPEDYVHRIGRTGRAGAEGAAVSLVDQEELPLLKDIERTMKRLIDKVEVPDFVPS
                     ATPDEGDTRPPREPRGTHTGSRGRHDKPRSEHARAAAPQGDAKAASGEGKPQHGQTGG
                     QGQGQKAGKPRHGKPPQQGKGQSQGQGQGQQGQGNGQGKPQHGKEQRQGKHGHGGQGG
                     QGSQPGHKAPSAPQGKKHGAHGGAHGGPNPNKAPRPREGGNVDDDFGNRVDYQPRAKT
                     NYRDPEAALNRRISGENYRASGFGKNGPRGNPNRGTPALLEEPVRQTVTLTGRRVGEV
                     KKTGLPKDVDGNK"
     gene            complement(4086..7175)
                     /locus_tag="PKX01_00855"
     CDS             complement(4086..7175)
                     /locus_tag="PKX01_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ74696.1"
                     /translation="MTRLRLPQRPLAVLLAAWPLAAPAMDAASSAPALSALAWIAVGA
                     LAAVAATVALRRRRGKVDADARTSADAESLRLQTLLHTIPDLVWLKDPDGIYLACNRA
                     FQTYFNAPEADILGRSDADFVGAEHAARVRDDDAAAVAAGTPTATERWSIHRATGAQV
                     AHEITRTPMFAADGSLIGVLGVARDVTERKKAERHLEEACQAAQRHLLEAEQSRSVLL
                     SLVEDLRAGEQALRDSESRFRAAFNQQFQLMALLAPDGTVLEVNELILHVLGGTRASY
                     LGKPFWSAPAWRQLPEWHSIWPERLARFQHGEEGPLLTEDVFQAADGSFRHADTATTA
                     IRNAHGSLVALLVQANDITGRRQAEDQLRKLSLAVEQSPTSIVITDLAGRIEYVNEAF
                     VRNAGYSRDEALGCDVQQLQDAIPLKDVRTSMWSALREGRIWGGQFINRHKNGDIFYE
                     YAQIAPMRQADGRITHYVWVKENITAQKRIGEELDRHRHHLEELVHQRTAQLEEARHA
                     AEVANQAKSAFLANMSHEIRTPMNAILGMTHLLRRGPTTPEQDDRLGKIIAAAQHLLS
                     VIDDILDISKIEAGKLEIEETDLDLATVLHRVADLVRDRARSRQVNLYVELPDPLPGP
                     LRGDPTRLTQALLNYASNAVKFTERGRIVLRARVEDADADNVQVRFEVEDTGIGIRAD
                     ALDRLFNAFEQADGSTTRRYGGTGLGLAITRRLAHLMGGEVGVTSAHGTGSTFWLTAR
                     FRRGAPATEPVAPPRPHATTRRPPGETRILVCEDNPINQEVALTLLKDAGYRADLAVD
                     GIDAVGKTAALRYDAILMDMQMPEMDGLEATRRIRSLPGYRDIPIIAMTANAFGKDRQ
                     DCLAAGMNDHLAKPVDPDALIATLDAWLNAAPGSPTTTTAPPATEPPVEAPLPDLSTL
                     PGMDLDFGLQVVRGNVRRYARLLHMLVEHHGHDATALRDALAAGRSSDAGRIAHSLKG
                     AAGNLGAERIRDLATRLNDRLRAQQPPDALQGDLDALDAALGEFRTAMASIPGAKDD"
     gene            complement(7172..8911)
                     /locus_tag="PKX01_00860"
     CDS             complement(7172..8911)
                     /locus_tag="PKX01_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ74697.1"
                     /translation="MQRHRYLRLSDIATTSVRTVLPDSPLRDAIALMAQQHVSCVVVT
                     EGLRPVGILTERDIVRVMSNGLRPATAIGDVMSQPLLTARMDLDFPAAQNLLARQGIR
                     HLVVTDDAGQLWGIVTESDFRAHLNRDLFSRIRSIESVMDPEFTTLPPDAPLQRALDC
                     MSTGRCDHVVVTRQGSPVGILTERDVPRLMLRGVNPSTTALDQVMSSPVQSIRTHDTL
                     ATATDRMDAAGIRHMVALDEQDRLAGVVSQHRMLERLGATLLDEMRSRLQDRLDVILD
                     ATGVGTWEYDYAAGRLTRNAALWKLLGVDAPSGRDETLDAAFARVHPDDLPRLRTIIE
                     AAREQRNGNDEPFSYEYRVLRPDGSWLWLRTIGRNVDRDDDGLALGAAGITIDISGEK
                     AATSRATESEHRFRRLMERLPIPVMHIARGGRCLFLNDHFQQIFGYTIDDLPHSDAWR
                     QRAFPDRDLRARADAAWREAFLTARRHGQAIAPQEFRVVCKDGSVRIVDASGIVLGDD
                     FLATFVDVTERKSVQEQLSLRLAELNRWYDAMLGREGRVLELKREVNALLRATGQPIR
                     YPSADQPSEGRPA"
     gene            9022..9891
                     /locus_tag="PKX01_00865"
     CDS             9022..9891
                     /locus_tag="PKX01_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase-like protein"
                     /protein_id="HMZ74698.1"
                     /translation="MPAGPPVSVSSSVLTLPSPFCPSDRIGLLESPECDRSAVLRRVE
                     TGDYDKPFVLDDGTTRSLHFSLALIQSTMSLKDPFALELDYTRAMMSFLLFLPRPRHL
                     LMLGLGGGSLVKYCHRHLGSARLTVVEIDPHVIAFRDEFEVPPDDGRLQVIQGDGAAW
                     VADCSERPDVILLDAFDRDGLSGSVSNETFYGQVCAALAGRGWLVANLAGDKAERVAH
                     RDMIAAAFGERVWSLTVPDGGNDIVLAARAEGFAPRWRELANQARALRARLGLDFPDF
                     VRRLERSQRLRWT"
     gene            complement(9898..10746)
                     /gene="fdhD"
                     /locus_tag="PKX01_00870"
     CDS             complement(9898..10746)
                     /gene="fdhD"
                     /locus_tag="PKX01_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMZ74699.1"
                     /translation="MPPLPPDECPPDEPPAPQATTTLTVRRLDGPQLRATEDTVAEEV
                     PVALVYNGISHAVLLATPADLEDLALGFSLSEGILATPTELFDSETVATPHGLELRLH
                     VAAGCFADLKARRRSLAGRTGCGLCGVESLAALARAPGPVTRGAPIPASAIAAALAAL
                     PAHQPLRATTGAVHAAAWIDRRGTLLALREDVGRHNALDKLLGHLARSATDPHAGWVL
                     VSSRASYEMVQKVATVGIGSLVAVSAPTALAIRLADAAGVTLAGFARDRRLVIYTHPD
                     GLLADA"
     gene            complement(11032..11652)
                     /locus_tag="PKX01_00875"
     CDS             complement(11032..11652)
                     /locus_tag="PKX01_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HMZ74700.1"
                     /translation="MHKKIVVSAIAAIAALSAGLAHAESPWMVRARAVNLDWDNKNAD
                     NLQNAGVGDVSAKKRWIPEVDISYFFTPNIAAELVLTYPQDVDIQTSALGKIGKVQAL
                     PPSLLVQYHFTELGAFKPYVGLGVNYTIFTKKSFDTALGTVSVDRASFGFAAQVGADY
                     KLDKNWSLNVDAKYIQMDTDVKLGGSKIGKLDLNPVTWGVGVGYRF"
     gene            complement(11881..13527)
                     /gene="lldP"
                     /locus_tag="PKX01_00880"
     CDS             complement(11881..13527)
                     /gene="lldP"
                     /locus_tag="PKX01_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HMZ74701.1"
                     /translation="MQTWMQIYDPAGNLWLSALVAALPIIFFFIALAVLRLKGHIAGT
                     ITVGIALAVAIFFYNMPVEMAFASAGYGFLYGLWPIAWIIIAAVFLYKITVKTGQFDI
                     IRASVISITDDQRLQMLLVGFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
                     LIANTAPVAFGAMGIPIIVGGQVTGIDPFKIGQMAGRQLPLLSVIVPFWLVAMMDGWR
                     GIKETWPAILVAGGTFAVTQFFTANYIGPELPDITSALVSLVSLTVFLKNWKPKNIFR
                     FDTQHHAELGEDQHYSLGQIAKAWSPFVILTVMVTIWSLKPFKALFAKDGPLYSLVLQ
                     WPVPMLDKLVIKMEPIVAKATPYAAVYKLDLLSATGTAIFLSALISMLVLGLRGGEAV
                     KTFKETVLELKRPIYSIGMVLAFAFVANYSGLSATLALLLAGTGKLFPFFSPFLGWLG
                     VFLTGSDTSSNALFCSLQATTAHQVGVSDTLLVAANTTGGVTGKMISPQSIAVACAAV
                     GLVGKESDLFRFTVKHSLLFATMVGIITTAQAYLFTGMIP"
     gene            complement(13586..16360)
                     /locus_tag="PKX01_00885"
     CDS             complement(13586..16360)
                     /locus_tag="PKX01_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HMZ74702.1"
                     /translation="MSALIDALRQRLPASRVITDELRRLAYGTDGSFYRLIPEVVTVV
                     HDEAEVRDVLDLARQHGRSVTFRAAGTSLCGQAVTDGVLVLLGEGFAACRIADDGAHV
                     AVGPAIVGAEVNRRLAPFGRKIGPDPASINAAKIGGIAANNSSGMCCGTAQNSYRTLA
                     SLRVMFADGSVLDTGDSASRAAFQKSHGALLQGLAELATAVQGRPALATRIRAKYKIK
                     NTTGYSLNALVDFADPIDILAHLMIGSEGTLGFLSEVRYHTVPEVAHKASALVFFPDM
                     ESACRAVVGLRQQPVDAVELLDRASLRAVADKPGMPPLLQTLADGATALLIETRAPDA
                     VTLDARIAAVLAELACHPLTEPAAFTTAPAEIERLWNVRKGTFPAVGAVRKPGTTVII
                     EDVAVAVEDLAACCLDLQRLFAAHGYHEAIIFGHALEGNVHFVFTQDFGIESEVARYA
                     AFMDELCTVLVLKYDASLKAEHGTGRNMAPFVELEWGSEAYALMKAIKQLFDPQGLLN
                     PGVIINDDAEAHLKHLKPMPAAGQLYAPVDRCIECGFCEPQCPSHGLTLSPRQRIVAW
                     RELSRRAAAGEAPDTLDADYTYMGLDTCAACGLCSTACPVGIETGALTRAVRGERLSG
                     VARRLGRLAAEHFGATQALARTALKAGHLAESVIGTRALARLSGGAWKRGMPHPQPAP
                     RASRSAGDPVVYFPTCAGRIFGADAPEQALSATVIRVMERAGYAPIVPDGVDALCCGQ
                     SFASRGLADEADRKSAELEAVLRAASRDGAIPIVLDASACSLRMKTFLAGRLPVLDLV
                     EFAHDALLPRLMLQRKADPVLIHLNCSARRMGFETQLKALAAACAEQPVLPPEVKCCG
                     FGGDRGFVVPELNAHALRKLEVPAGCCGGYSSNATCEIGLTDTTGLPYRSIVHLLDEC
                     SAEAARIATC"
     gene            complement(16463..17914)
                     /locus_tag="PKX01_00890"
     CDS             complement(16463..17914)
                     /locus_tag="PKX01_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HMZ74703.1"
                     /translation="MTAPRPAGDARPITIVSAEALRQRIHGAVNDPHLRSSFRGAMDF
                     LMAKRAAQFPDADELASLRSLSESIRQYSLSRLPDLLEQLEARLTANGVHVHWAETPD
                     EANAILLDIARRHAAQLIVKGKSMVSEEIEFNHAMHAAGIDALESDMGEYIVQLAGEK
                     PSHIIMPAIHQTKQQIARLFADKIPGVDYTDNVDALIQIGRRVLREKFETADIGLSGV
                     NFAVAETGTLCLVENEGNGRMCTTVPRVHVAITGIEKVVEKLEHVAPLFSILTRSATG
                     QSVSTYFNLISGPRKPDERDGPEEVHLVLLDNGRSQAYADAQLRKTLQCIRCGACMNH
                     CPVYTRIGGHAYGTTYPGPIGKIVSPHMMGLQQTHLMPSLSSLCGACGEVCPVKIPIP
                     ELLIRLRGEAQSAPQANPVMAGQGAGHDRLVSFAWRAWARVYASPLGYRTFSWLASRF
                     RALTPTRQAGWTAARLPLQPAPRRLRDMLRDRR"
     gene            complement(17911..18585)
                     /locus_tag="PKX01_00895"
     CDS             complement(17911..18585)
                     /locus_tag="PKX01_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HMZ74704.1"
                     /translation="MSARNSILARLRATRPAAADPLPDLDAHFAPRARHETVAQRASR
                     FRSGIEAFHAEVHVCADADWPDLLARLCAAKGIGTLLYGRNTPAGERIDATPFGATQI
                     RPWQGRFEELKADLFHRIDAGFTTARGAIAETGSLILWPSTAEPRTLSLVPPVHFVLL
                     DARSIHATFFDALRAEQWSDRLPTNALLISGPSKTADIQQTLAYGAHGPKELVVILTH
                     AEGASA"
     gene            complement(18582..19397)
                     /locus_tag="PKX01_00900"
     CDS             complement(18582..19397)
                     /locus_tag="PKX01_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ74705.1"
                     /translation="MSDTLPGGGPTPRRYPSRPAAVYLYATCLVDLFVPEAGLDAVTL
                     LEREGLRVIFPENQTCCGQPAYTSGFPDEARQVVASQLDLFPEDYPIVVPSGSCAGMM
                     RWHWRGVVGDDPALAARAAALAERTFELAEFLVHVLDFRRPDTGAPTTVTLHTSCSAR
                     REMGTLLVGRELLARLGQVTLAVHDHESECCGFGGTFSLKHPDVSTAMVGDKVNALRA
                     TGAAQMVTADCGCMLNISRRAAKEDRDAGRVGASLPAEHLASFLLRRTGGKPA"
     gene            19680..20456
                     /locus_tag="PKX01_00905"
     CDS             19680..20456
                     /locus_tag="PKX01_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMZ74706.1"
                     /translation="MSSSKIAVQRLSDTIAHELERRILEGSLKPGDRLQAERELAAEL
                     GVSRPSLREAIQKLVSKGLLHSRQGGGTFVTDRLEAGFTDPWSEMLRQHPDIQSDLLE
                     FRRMLETESAGLAARRATDGDLQRIGDAYARVEGLFAQGPSAEVFAEQVRADLAFHQA
                     IAEASHNVMIGHLTASLFRVTHDNIDRNLRHLRRLEDDWQELRRQHAAIWEGLRTRNA
                     GAAQAAVERHIAFVEESMAATARQAERERRAQTVRIRSPE"
     gene            21497..22369
                     /locus_tag="PKX01_00910"
     CDS             21497..22369
                     /locus_tag="PKX01_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ74707.1"
                     /translation="MQNSSSVNFGAPLRAKLESYEAISRVEARSGKSAREIAGYAAAA
                     GAALAMAGNADATVIYSGVQNLVAQIDPAHQFGVSYVNGHAGYSVNFQNFSVNGVPFM
                     AGAVIAAELGTNINNNAGLAAKYIGAAVVQGSGVQFVAGSSSPLGLATPVAPGAAIGA
                     GSNQFGGAAGAVRMGVGYVNGKTVQYGNLLLGDNLFGFALPNGNFGWIRLNIEDLGLN
                     QPFSDILQNAGGISPLFNGQGFPDRITVVDWAYDDQGRTIFAGTLNVPEPGALSLLAA
                     GAAGIATMRRRKLH"
     gene            22419..22937
                     /locus_tag="PKX01_00915"
     CDS             22419..22937
                     /locus_tag="PKX01_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74708.1"
                     /translation="MHRHVDSSRFGAYRIENVHLLTSSEQRQEIVQFWLRERAIPTLP
                     EAVRRAHEVCLLVRRDDGTGALAGVSTVGRMRTRSGLIYWVFRMFLRKPDRVPRLSLA
                     VANATCAHLRLRARREGDAAGMLIVTENVKLMRPGARMLFQRQGCRYVGRNARGQDVW
                     VVDFPAHATAAA"
     gene            23015..25912
                     /locus_tag="PKX01_00920"
     CDS             23015..25912
                     /locus_tag="PKX01_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ74709.1"
                     /translation="MNASRKVLLVGWDAADWKIINGLVDRGLMPHTASLIERGVMGNL
                     ATLHPVLSPMLWTTIATGKRPFKHGVLGFTEPSPDGSGVQPVSQWSRKTKALWNILNQ
                     SGLKSHVIGWWPSHPVEPINGVMVSNHYHTALGPPDQPWPLAPGMVHPPRAQQALADV
                     RLNPNELLPEQVCFFVPRAAEIDQDKDRRLASVMKTLAECTSVHAAATWAMANEPWDF
                     MGVYYDAIDHFSHGFMRYHPPRRPFVPERDFELYRHVVEAAYQFHDLMLGTLLQLAPP
                     DTTVIICSDHGFHSDHRRPAQIPNEPAGPAVEHRDLGMFLIAGPGIRRDHLIHGASVL
                     DVTPTVLTLFGLPVGEDMDGKVLLDVFDRTPEVAHVPSWDAVPGDDARLSGEQQFDPL
                     AAKEVMDQLVALGYIEKLDDNREQAVAQTVRELRYNLARSYMDAGRMLDAAPLLQELY
                     AAEPDQYRFGVHLALCYRALNRVAELRPLVERMTRDRQEAAQTARAALQALCDTVRQR
                     QQAAATTTAVRAQDDPPSAADALASIDLSQLTEDERSQVDELRFKAWFSTSDLDYLMG
                     WVLGAEGRHDEALAHLLKAERADPGRPGLLVQIGEAHLAVHRWDEAEAAFRRALAADA
                     LSPYAHLGVARALLPRRARAEEAVEAALDSIRLLYQNPLAHYVLGLGLLRLRQFFMAA
                     DAMRTALALNPNFVRAHRVLARFYRLGTGEHDKARAHLEAIQHIRLGKRAASMTGQPG
                     LGEAESLAHEVDDEAGRPGLHADAVFAVRKTPDDPAEFVTVVAGLPRSGTSMMMQMLA
                     AGGLPILTDNKREADLDNPRGYFELEAATRLRTDRTWFREGRGQAVKIVAQLLPYLPG
                     DVPCRVIFMERDLDEVLKSQTVMLDNLGREGARLSDAQVQASYRQQLRRVKLWLARQS
                     QVDVLFVRHRDALADPAAVAEQVNAFLGGRLDCLAMATCVDASLYRQRATA"
     gene            25909..26607
                     /gene="cysC"
                     /locus_tag="PKX01_00925"
     CDS             25909..26607
                     /gene="cysC"
                     /locus_tag="PKX01_00925"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HMZ74710.1"
                     /translation="MTTDTQRPTVIWHPGDVTPAMRERLNGHRGRVLWFTGLSGAGKS
                     TLAHAVEACLHEQGRRAFVLDGDHLRHGLCAGLGFSSADRSENLRRAAEVARLFADAG
                     TVTLAAFISPLREDRERVRAIVGADRFVEVYVACPLAECESRDVKGLYKRARAAEIPH
                     FTGISAPYEAPEHPDLVLDTSRTPIEACVAQVLACVDRIVSLEADTFTDIGESMHPAP
                     TDRAPLTRSQRVEV"
     gene            26637..26828
                     /locus_tag="PKX01_00930"
     CDS             26637..26828
                     /locus_tag="PKX01_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74711.1"
                     /translation="MRTTPDINATPAAGHWQDFSAPVTLTAEQAAALYRSVNDVATLL
                     SGAIQLAENADSTLEALHG"
     gene            26856..27272
                     /locus_tag="PKX01_00935"
     CDS             26856..27272
                     /locus_tag="PKX01_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74712.1"
                     /translation="MQTERYGTRDQAYSAWHRCQSTRRYVGIEQAARLSMIDLDGALF
                     VEYDNGDREPLALIETARDVGQPVKPCTVTARLAQRSGLPAYVVLYRTSDLPNPADER
                     QPDIQSFRVRRIWPHPEPAWRVLSPRSWAEALLQIR"
     gene            complement(27553..28458)
                     /locus_tag="PKX01_00940"
     CDS             complement(27553..28458)
                     /locus_tag="PKX01_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HMZ74713.1"
                     /translation="MTIPGTQYSLFESVEAIPVEQPAQEPAGRRFSDYIVYVDESGDH
                     SLASIDQGYPVFVLALCVFHKRHYAEKIVPAVQKLKFNYFGHDSVVLHENEIRKQKGD
                     FAFLSHLPTRDEFMDRLSSVMGASNFILISCVVDKVRLSRNEGANSNPYHIALGVCLE
                     ALREFLAEKKQDDFKTHVLVECRGKKEDAELELEFRRICDGDNPSNRALPFDIVFADK
                     KTNLAGLQLADLVARPVGINYLRPEQGNKAFEMLKKKFFCDGGRNKVGCGYENVGLKI
                     YPPKKAKSPGEPTEATTPTGNPQST"
     gene            complement(28540..29085)
                     /locus_tag="PKX01_00945"
     CDS             complement(28540..29085)
                     /locus_tag="PKX01_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HMZ74714.1"
                     /translation="MCANYLPSSREELTAFPLPPPDFGYGEAYPGSIVPIVTTVAPCL
                     WVPASFGLVPMWAKDARIVRSTYNARSETVGEKPSFCSAWKRGQLCIIPAAAIFEPCY
                     ESGNAVRWRIERADGKPMGIAGIWERRLQDDVFGRLPVAIPSPDVAEAFGSVVTNVQQ
                     RIAANHPKPKPSLPSAIPCPA"
     gene            complement(29322..29398)
                     /locus_tag="PKX01_00950"
     tRNA            complement(29322..29398)
                     /locus_tag="PKX01_00950"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29362..29364),aa:Met,seq:cat)
     gene            29453..30787
                     /gene="miaB"
                     /locus_tag="PKX01_00955"
     CDS             29453..30787
                     /gene="miaB"
                     /locus_tag="PKX01_00955"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMZ74715.1"
                     /translation="MKKLYIRTFGCQMNEYDSDKMADVLGAGETLVKTDNPEEADVIL
                     FNTCSVREKAQEKVFHDLGRVKHLKQKNPGLIIGVGGCVASQEGEAIVQRAPYVDLVF
                     GPQTLHRLPKLIAERKATGRPQVDISFPEIEKFDALPPARTEGASAFVSIMEGCSKYC
                     TFCIVPYTRGDEVYRPLADILAEVAGLTAQGVKEITLLGQNVNAWRAPLDGAAGEMAD
                     FAFLLECVHEIPGVERLRYTTSHPREMTARVVDAYARLPKLVSHLHLPVQAGSDRVLA
                     AMKRGYTVLEYKSLVRRLRAARPDISLSSDFIVGFPGETEADFEQTMKLIDELGFDTS
                     FSFVYSPRPGTPAADMADDTPQDLKLARLARLQKAVEAHAARISEAMVGSVQRILVEG
                     PSKKNPAELAGRTDNNRVVNFAGNSRMIGHFVDVTIVSALPHSLRGELVLKD"
     gene            30836..31795
                     /locus_tag="PKX01_00960"
     CDS             30836..31795
                     /locus_tag="PKX01_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ74716.1"
                     /translation="MKNKALEVAFEPADNTRLANLCGVLDENLRQIATAYDVEIARRG
                     ERFSVRGHLVQARLAAQALQHFYDLAGEHLSIDTVQLGLIELANRSQASHSAPALLTR
                     KTELHGRTPRQVDYIRQIQEHDITFGVGPAGTGKTYLAVASAVDAFEREQVERIILTR
                     PAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVAKLFERGCIEIAPLAFMR
                     GRTLSRAFIILDEAQNTSPEQMKMFLTRIGIGSRAVITGDLTQVDLPRGQRSGLGEAL
                     QVLADVRGVAFTRFLKEDVVRHPLVARIVAAYESHSATPAPTT"
     gene            31792..32274
                     /gene="ybeY"
                     /locus_tag="PKX01_00965"
     CDS             31792..32274
                     /gene="ybeY"
                     /locus_tag="PKX01_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMZ74717.1"
                     /translation="MTASALPRRLDLSVQYACHRDGVPLRADFRRWLRAAESGAARIT
                     VRIVDEAEGRELNRDYRGKDYATNVLTFAYAEGEDMPMPEGLPLMGDLVLCRSVVERE
                     AAEQGKPLAAHYAHLVVHGMLHLQGFDHEADADAELMEARERAILAELGYDDPYALEH
                     "
     gene            32347..33189
                     /locus_tag="PKX01_00970"
     CDS             32347..33189
                     /locus_tag="PKX01_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HMZ74718.1"
                     /translation="MEPSSSKPSLLERLSALLTREPEDREELLHLLHAAHERNLFDAD
                     ALAIIEGALQVSDMRVADVMIPRAQMDVVRVDDSIEDITRFAITAAHSRFPVIGEGKD
                     DVIGILLAKDLLRVFAGEAFDLRDMLRPAVFVPESKRLNVLLREFRVSRNHMAIVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEAADNIRLDQAGRYRVKAGTEIDDFNAAFG
                     TTFSDDEYDTVGGLLIRKLGRLPKRNEVIVLDNLRFQILRADSRRVYSLLVERTPQAG
                     VIGG"
     gene            33189..34685
                     /gene="lnt"
                     /locus_tag="PKX01_00975"
     CDS             33189..34685
                     /gene="lnt"
                     /locus_tag="PKX01_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HMZ74719.1"
                     /translation="MSRLRPVLAWAAGAASVLAYAPFEYFPLAAVALAVLYALLGRAA
                     DAAITPGARRRAGFLIGWAWGFGALGAGVSWLYVALHRFGGMPMPLAGSAIALFCAVM
                     ALFAGGAGALFADLRRGRPWADAALFGALWLLTEWTRGWLFTGFPWLVSGYSQTPPSP
                     LAGFAPVFGVFGVGGLLAWIAALLAEAVTRRRIACAGLVLLVGAGGAALRPVAWTVPV
                     GAPLQVALLQPNMEQDLKWRPDMLPRWLDIHLELLRNNPATLVVLPETTLPLLLEHLP
                     DGYLDALTRSAAVGGDAILGAFVRDGDGRIFNAAVSVGRSPTQHYAKHHLVPFGEYSP
                     PLFRWFYDLVHIPMADQTRGAEVQPPMSLAGQKIAVNICYEDVFGEELIRALPEATLM
                     LNLSNLAWYGDSFAQPQHLQIARLRALESGRPMLRATNTGMTGLVQPDGTVSAALPAF
                     TRGALQVAVRGYTGSTPYGRWGNGPAVGLAALVVLVVLAGRVRGRTPI"
     gene            34753..35151
                     /locus_tag="PKX01_00980"
     CDS             34753..35151
                     /locus_tag="PKX01_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HMZ74720.1"
                     /translation="MMRPYPTNSPEALARLVALAILADGRLHNQEVTWIREHDTASLI
                     GVEQDTLVQVLLDCCRDLLSEGGRGRVDLLSEARLAPLAEEIADPALRKVAISAVLVV
                     AKSDGRLSSEEQTLLRFLLSRWDIELQELV"
     gene            35256..36629
                     /locus_tag="PKX01_00985"
     CDS             35256..36629
                     /locus_tag="PKX01_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HMZ74721.1"
                     /translation="MSDQIKYLLDESRLPKAWYNIAADLPVPLPPPLHPGTLQPLGPA
                     DLAPLFPNAIIAQEVSTERYVDIPEPVRDVLRQWRPSPLFRARRLEKLLDTPAKIYYK
                     YEGVSPAGSHKPNSAVPQAWYNAQEGVKRLSTETGAGQWGSSLAYAGGLFGLDVTVFQ
                     VRVSYDQKPYRRALMETWGARCIASPSLETESGRAVLAKTPDHLGSLGIAISEAVEVA
                     AKRDDTKYALGSVLNHVLLHQTIIGQEAIAQLEMAGDDPDVVIGCTGGGSNFAGIAFP
                     FIGLQLRGGANARRRRILAVEPSACPSLSRGQYAYDFGDTAHLTPLVKMHTLGSEFTP
                     PGFHAGGLRYHGMAPLVSHVKELGFVEARNYHQNACFEAGVMFARSEGIVPAPEANHA
                     IRAAIDEALRCKAEGRSETILFSLSGHGHFDMASYTNYLAGKLVDQDYDEQELAMALS
                     GLPRVPV"
     gene            36629..39313
                     /locus_tag="PKX01_00990"
     CDS             36629..39313
                     /locus_tag="PKX01_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HMZ74722.1"
                     /translation="MAPERAAAWHARSPQDVLDALRSSPHGLTTADAAERLRRHGPNR
                     LPGRRGPGPWQRLAQQFHQPLIYILLASGAIALLLGEWADAGVIFGVVLVNALIGFVQ
                     EGKAADALAALARSVASTVTVLRDGRMVACEAVELVPGDVVRLGAGDKVPADLRLFDA
                     RSLQIAEAALTGESVAVAKDTATVPADGVLAERACMAYAGTLVTAGQGCGVVVATGAD
                     TEVGRIAHLLDVTDALATPLTRAMAGFSRVLLWLILGLAVFGFVVGWMRGEALADMLM
                     AAVALAVGAIPEGLPAAVTVTLAIGVARMARRKAIVRRLPAVETLGSTTVICSDKTGT
                     LTENRMTVKVLWQGGRSFEWAENTPEPSAEPGRAGLPAGLNELLVAGVLCNDARLQAD
                     AAGRPASGDPTEVALLVAADVGGLDGAALRGACPRLGEIPFDAARPYMAVKVCVADHP
                     VVFVKGGLEALLPRCAAQIGVDGCPEALDPAAVEVAATALAADGLRVLAFARGGDGTL
                     GAELPAQGLVFLGLQAMQDPPRPAAIRAVAACRAAGVTVKMITGDHAATALAVARQLG
                     IAADGDAVLTGRDLAGLDALALQAAAMRVNVFARVEPAQKLRLVQALQAGGEVVAMTG
                     DGVNDAPALKAADIGVAMGAGGTEVAKEAAAMVLTDDNFATLEAAVEAGRGVFDNLVK
                     FILWTLPTNFAEGLVILFAVLAGTTLPITPLQILWINMTTAVLLGMTLAFEPIEPDVM
                     GRPPRPPGAPVLDAVQMRRVVLVGALLVAAAFGLFLRWRGFGASLEEARTIAVNVFVA
                     GEVAYLFNCRSLRGGFWRGGVLANPWFWLGIGAMVALQAAFTWWPAMNRLFASAPPPP
                     AAWLEIGAAAWALALAVGLEKRLTGGSASRPGAARIAR"
     gene            complement(39310..40935)
                     /locus_tag="PKX01_00995"
     CDS             complement(39310..40935)
                     /locus_tag="PKX01_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ74723.1"
                     /translation="MNLSELRVATRMRILVALTLIGLVALCTVSLFRLHASMMDDRKQ
                     QTRYVVQLGLGVLQHFHALAQSGALSDADARKAAIETLRTLRYEGTNYIFVVSTTSHY
                     ILMPPRPEKEGTDASGTKDSTGKAIIPELVKAALAGGGFVDYLFPKPGGTDPQPKVSF
                     AAQFGPWQWVLGTGIYVDDVDVEFRAIAWTLGGISAVLLIVLGVAGWGVGISIIRQLG
                     GEPREATAIMQQVADGDLSLRLEDSRPGSMLHALSTMIASLRTMMGEINRSAVQLVSN
                     ADHISQVSGEVADAAIRQSDATAAMAAAMEELTVSSSHISSSARDTEDNSQEAMRLAA
                     EGTQRVQQASGAIRKMSETVLGASTRIRALEGRISQVSSIANVIKEIAGQTNLLALNA
                     AIEAARAGEQGRGFAVVADEVRKLAERTSSATTEIEQMIVGIQSDTGSAVEAMNAALP
                     EVDEGVSLAASAAESLAAIENGATRTLERVHEIADATREQSAASTAIAQRVEDISNMV
                     ENTTKTIRGAAEAAVGLERIAGSLRDQVSHFRV"
     gene            41169..42077
                     /gene="glyQ"
                     /locus_tag="PKX01_01000"
     CDS             41169..42077
                     /gene="glyQ"
                     /locus_tag="PKX01_01000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HMZ74724.1"
                     /translation="MSVHKPTFQQIILRLSNFWAERGCTLLQPYDIEVGAGTFHTATF
                     LRAIGPEHWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPANIQELYIDSLR
                     ALGIDPNAHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLSCKPVLG
                     EITYGLERLAMYLQGVENVYDLVWSIGPDGTPVTYGNVYHQNEVEQSTYNFEHSNVEW
                     LFRLFGEYEAEAKRLMAVPLVLPAFEMVMKCSHTFNLLDARGAISVTERAAYIGRVRA
                     LAREVAQAYFNSREALGFPMLKKEAA"
     gene            42077..44161
                     /gene="glyS"
                     /locus_tag="PKX01_01005"
     CDS             42077..44161
                     /gene="glyS"
                     /locus_tag="PKX01_01005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HMZ74725.1"
                     /translation="MTGATLLVELLTEELPPKALPRLGQTFAKGVVEGLRARGLAAAE
                     GAFRWFATPRRLAVSVSGVVAEAAARDVTEKIMPVSVALDAAGQPTPALVKKLEAKGI
                     PLSAVATFERRLDGKAEALFHTATVPGARLADVLAGIVQDALKALPIPKVMRWGDSDV
                     AFVRPVHSLLMLHGADVVPGTVLELAAGRVTRGHRFMSRGEIVVATADAYEPTLLAEG
                     KVVPDFAERRADIEQQLVREAARQTASLGQYADLLDEVTALVEHPTVYVGEFEAEFLA
                     VPQECLILTMRANQKYFPLFDAQGKLLNRFLIVSNMRLEDPSNIVTGNQRVVRPRLSD
                     ARFFFEQDRKAGLSSRVGKLDSVVYHNKLGSLGDRVQRLDAVARAVAVRLGADDAQAG
                     QAARLCKADLVTDMVGEFPELQGIMGRYYALHEGAQAVVADAIEQHYRPRFAGDALPD
                     SNIGRALALADKLDALVGFFGIGQVPTGDKDPFGLRRAALGVLRILMEAPLPLDLAAL
                     IADAVASFPPGVLTAEVDAPLFDFMLERLRNLLRDAGHAPAAVDAVLALRPTRIDLVV
                     PKLEAVAAFQQLPEAQALAAANKRIVNILRKAGGEFAEPEVALLAEDAEKALFHAIVD
                     VAPLARSHFQNEAYTDALRVLAGLRSAVDRFFDEVMVMAEEPLVRQNRLALLNQLAGL
                     MNQVADISRLSA"
     gene            44179..44712
                     /gene="gmhB"
                     /locus_tag="PKX01_01010"
     CDS             44179..44712
                     /gene="gmhB"
                     /locus_tag="PKX01_01010"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HMZ74726.1"
                     /translation="MKIIVLDRDGVINQDSDQFIKSPEEWKPIPGSLEAIARLNESGW
                     RVVIASNQSGVGRGLFDMDTLNAINEKMVKALAQAGGRLDAIFFCPHAADSTCDCRKP
                     KPGMFRQIAERFNVDLRGQPVVGDSLRDLQAGVAVGCAPYLVLTGKGGKTRLDPDLPE
                     GTQIFPDLAAVVAQITA"
     gene            44723..45490
                     /locus_tag="PKX01_01015"
     CDS             44723..45490
                     /locus_tag="PKX01_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ74727.1"
                     /translation="MVLIRNLLFMLALAVFTPLYALIGVLSSPFGPQTRHKLIRGWPR
                     IMGWVIKHVLGIPYRLVGAENIPREGPAIIVSKHMSAWETILLQDIFPPMVFVLKKEI
                     HSIPFFGWGIARMPMIAIDRAAGKDALTQVEAQGRERLAEGFWVTIFPEGTRVAPGSK
                     KRYKAGASWLAAQTGVPVVPVAHNAGEFWPRNALFKRPGVVTVSVGPVIDTKGLNAEQ
                     INAKAQAWIEGEMRQLFPGHYRPTRQRAAEAVEPAAE"
     gene            45487..46236
                     /locus_tag="PKX01_01020"
     CDS             45487..46236
                     /locus_tag="PKX01_01020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HMZ74728.1"
                     /translation="MSLPASPRKDGLQREVLLDGVAIAYTLKRSARRTFGLRVDARGL
                     TVGVPLRATQRAVDAFLHDHARWILDKVQVHAARAEAPPFAVVEGAGLPVLGETWTLH
                     LSKGLNRLVWHEGGVTADAPRILEIALRAGSDPAAVLRRGVQQRALEVFRHRVAEFCL
                     RIGQPVPTVGLSSARTRWGSCSRRSGIRLHWRLIHLPLELVDYVAAHEVAHLLEMNHS
                     PRFWSVVESVYPEHVVARARLRAVAASLPQL"
     gene            46251..46643
                     /gene="gloA"
                     /locus_tag="PKX01_01025"
     CDS             46251..46643
                     /gene="gloA"
                     /locus_tag="PKX01_01025"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HMZ74729.1"
                     /translation="MRILHTMLRVGDLERSIAFYTEVLGMRLLRRSDYPDGQFTLAFL
                     GFQDEADGAVIELTYNWGVTSYALGTAYGHIALEVPDAYAACAEIKARGGKVTREAGP
                     MKHGSTVIAFVEDPDGYKVELIQAKNRG"
     gene            complement(46689..49796)
                     /locus_tag="PKX01_01030"
     CDS             complement(46689..49796)
                     /locus_tag="PKX01_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ74730.1"
                     /translation="MKALFDRLASLLGLDSLRQQLTLLFTVLLGLAIGIYALYTAVEQ
                     AAYVERLERQRAEESARHLADDLAPHLTAGDRTGLTHHLEALARQRDMLHLTVTDLHG
                     LPVAGVSVTEGAPRVDAAADRPLALPADTQPTRQGETDAIVVWAEIRDDVPRGWLRLE
                     IAPTAAREALAHIVEDSLITGLLTVTLGAVIIRRFLRGPLHSLERATAFAEGLETGEG
                     AVLNEPSRLEEIRKLVDALNWTSIRFYDGQSALADSESRNRAITEAALDCIVTVDDGG
                     RIIEFNPAAERSFGVSRADALHQYVGDLIFSDERRDEQERILQQVLDPERRDEAGQRF
                     ETLVTRAGGQTFPVDVAIVPAISSGRRLFTAYLRDISDQKRAEEAMRMAMEAAEANSR
                     LKSDFLANMSHEIRTPMNAILGMTDLALDTDLDAEQREYLTLVKSSAQSLLNIINDIL
                     DFSKIEAGKLDFEHIDFSLRDCISLATRTLQQRAAEKDLQLVTQIEPQTPDTLRGDPH
                     RLRQVLINLISNGIKFTPHGEVRIRVHPTPGNKDAGRIELTFSVQDTGIGIPRDKQGL
                     IFDAFSQADTSTTRRYGGTGLGLAISAQLVKGMGGGIRVTSEPGQGSTFEFNAVFEPG
                     QSVVPVETDTELEGLPVLVVVKSASDRGILSELLTHWRMSPLMVSDASAAHRELLAAA
                     KRGSPYRVALLGRDLPDMDGFDLASALSAASPRPLSVILLAERGRRGDGARCREMGIA
                     AYLPMPIQASDLLNAILLSCDVGGEADGDRPLITRHSLREQKRVLNILLAEDNPINQT
                     LALRLLAKLGHQVTVANNGAEALELHARQRFDIILMDVQMPVMGGFETTAEIRRREAA
                     GTPHTPIVAMTAHAMKGDRERCLDAGMDGYLSKPINAPDLVEALIHHADQSDPPPQPM
                     AHETVALGPVFDRAAVLANLGDDEELLTQLIAMYLHDEPRMVGELENAIAHQDAEAVN
                     AAAHALKGAVANFFACRAQARAMQLEQLGRHKHLSDAPAVLAELKRELAALHEAFRSV
                     APADA"
     gene            complement(49793..50833)
                     /locus_tag="PKX01_01035"
     CDS             complement(49793..50833)
                     /locus_tag="PKX01_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ74731.1"
                     /translation="MAIAIVDDTPLNLTLIQALVRKIAPPDTEIRCFTSPLEGLQWCS
                     EHEPDLVIVDYMMPDLNGIEFITCLRKVHPAEAVPILMVTAAHEKEIRYQSLTAGASD
                     FLTKPIDRYEFDPRVRTMLALRLSHLRMRHYNDDLREAVRRATADIVARERETVTRLA
                     RAAEFRDPETGAHILRMAHYSRLIAEQLGLGAEFCERLLSAAPMHDVGKVGTPDHILL
                     KPGRLTDEEMAIMRQHASIGHDILKGSSSPMIQLAAEIALSHHEKFDGSGYPGGLTGE
                     AIPLSGRIVAVADVFDALTSERPYKPAWALDRAVQFLKDGRGAHFDPACIDAFLQRWD
                     EVLAIRDRYRDE"
     gene            complement(50880..54011)
                     /locus_tag="PKX01_01040"
     CDS             complement(50880..54011)
                     /locus_tag="PKX01_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ74732.1"
                     /translation="MFAISPSAALALSLAISMLLAASLRYVAAVERPQTALRAWAAAF
                     ACHAVHDAALLAPALTHLPLALLAPVGLMGMSAALWIGLGRVTGDGHSPRVMLLPLLA
                     ALWALVPTAPGRSEVLRTLPVDAVAITFTFAAARVFWRRATHQPGTGHRLAAVGLGGW
                     GLVLLAGSAGVLADGSVARALAAALALSGLGCALLINALCLQNAAEARLTTHLRAEMD
                     RRIAAESALAESHSRFTALAESSDLGLFVVSPDGQFQYTNSRYRALSGLTDEELRTRP
                     DTQRMDGEDAARVSAAWAEARALRCQTHYERRVVQSGATEGWLSVHVAPITAGDSLQG
                     FVATVEDITARKQAEAALRDSEERLSQVFRLLPDAVMLSDPDSGRIDDVNQGWTDTLG
                     HPREATLGRTAEALGLWCNPSDARTVLDTLRAGHDVSDQPATLCHRDGRVLETLLRAR
                     LIRVRGRDGLLTVIRDVTEPRRLERIGRAAADALRVSEAMFSAAFRASPDYLTISRLD
                     GHLLEVNPTFERMTGWRRDEAIGAQAVELGLWAFPEEREAMLHELSLHGAVTDFPCTL
                     GTRWKTRRTCLLNVSMVTLENEPCLVGMIHDITEQQAAEKALRDSERKFATIFNLAPF
                     ALSLTRVGDRTHVDANPAWERLFGIERNTVLGRTSGEFGCWTDPRAYDDLYARVVSSD
                     VLAQQEIRIRPCNRDDVATCLISGQRLRIRDEECALWAMVDISELRRAQARIEELNQG
                     LELRVVERTTELSNALEALRRTQEDLVRSEKLAALGSMVAGIAHELNTPLGNSVTVTS
                     TLATRTDELTHRKAAGPLRRSDLESYLDSVRTASDLLLRSLSRARDLVSSFKQVAVDQ
                     TSEQRRRFELGEVLREVVTTLGPMFKGTPYTLRIEPAPALEMDSYPGPLGQVITNLVS
                     NALVHAFAGRDHGTITLTPAADGPAGVTLCFRDDGHGIDPAHLGRIFDPFFTTRLGTG
                     GSGLGLHIVYNMVTRVLGGRLEVSSQLGVGTTFTLAMPRTAPATGGTENPMSISAKPA
                     GRSFNFEDNGPII"
     gene            complement(54029..54811)
                     /gene="fliR"
                     /locus_tag="PKX01_01045"
     CDS             complement(54029..54811)
                     /gene="fliR"
                     /locus_tag="PKX01_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HMZ74733.1"
                     /translation="MLTVTAAQLDAWLALFAYPLARILAMVSSAPVFGNTALPRRIRL
                     VAGLALALGIVPALAPIPAIPLGSWSGLLTIFQQTLVGLAMGLAMRVVFAALDVLGEL
                     TSLQMGLSFATFFDPGSNGQTAVVGEFLTLLATLIFLALNGHLLMADVLARSFEWLPV
                     NALPLHAGGWAVLVRFGAVTFSAGLLLALPVVTALLITNIALGILTRAAPQLNLFAIG
                     FPITLTVGFGVIMLALERFAPLLQRFYDMGFDTIAALLKALA"
     gene            complement(54814..55083)
                     /gene="fliQ"
                     /locus_tag="PKX01_01050"
     CDS             complement(54814..55083)
                     /gene="fliQ"
                     /locus_tag="PKX01_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HMZ74734.1"
                     /translation="MTPTTVIDLGRQALELTLLVSAPLFIAALATGLVVSIFQAATQI
                     NEQTLSFVPKLVATFITLVLAGPWMITLLTDFIRRLFESIPAMIG"
     gene            complement(55108..55848)
                     /gene="fliP"
                     /locus_tag="PKX01_01055"
     CDS             complement(55108..55848)
                     /gene="fliP"
                     /locus_tag="PKX01_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264540.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HMZ74735.1"
                     /translation="MTAARLRRLTPLLLLLPLPALAQALPALTSGPAPGGGTQYSLPV
                     QTLLLLTALSFLPAMLLMMTSFTRIIIVMSLMRHALGTQTSPPNQVIVGLSLFLTFFI
                     MAPVGDRIYTDAWVPLQENRISFDQAVERASVPVKGFMLRQVREPDLNLFSKLANTPK
                     VDKAEDLPLRVVIPAFVTSELKTAFQIGFIIFIPFLIIDMVVASVLMSMGMMMMSPAI
                     VALPFKIMLFVLVDGWNLLVASLVQSFG"
     gene            complement(55845..56252)
                     /gene="fliO"
                     /locus_tag="PKX01_01060"
     CDS             complement(55845..56252)
                     /gene="fliO"
                     /locus_tag="PKX01_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HMZ74736.1"
                     /translation="MRRALVPAVLLSIGPVALAGDAAPDLTGSVVQTLLALGAVIGLL
                     FGTLHLLRRSGVARAGGAGLLQVRAATAVGPRERVVLVEVAGKLLVLGVAPGRVTPLH
                     TLDATDLPAVPSRPAGERDFQSWLRQTLERRAP"
     gene            56418..56624
                     /locus_tag="PKX01_01065"
     CDS             56418..56624
                     /locus_tag="PKX01_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74737.1"
                     /translation="MLLWRREVQPVVKPVPEAVPVSAQADYEEKRQRALQILGERWLL
                     HRANAPDRRPVRSVLGGAAGRPLS"
     gene            complement(56694..57152)
                     /gene="fliN"
                     /locus_tag="PKX01_01070"
     CDS             complement(56694..57152)
                     /gene="fliN"
                     /locus_tag="PKX01_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HMZ74738.1"
                     /translation="MSDTETDAPDDNQVSDDDWAAAMQEQAASEGLTDPATGVDESPY
                     QAKPASQLFPDFGQAGTKSGTLNDFDMILDIPVQLTVELGRTKIAIRNLLQLAHGSVV
                     ELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERIRKLRS"
     gene            complement(57156..58157)
                     /gene="fliM"
                     /locus_tag="PKX01_01075"
     CDS             complement(57156..58157)
                     /gene="fliM"
                     /locus_tag="PKX01_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HMZ74739.1"
                     /translation="MSQDFLSQEEVDALLKGVTGETDEPDSGEESQDGVRDYNLATQE
                     RIVRGRMPTMELINERFARYLRIGLFNYMHRNVEISVGPIKVQKFSEFVRNLVVPTNL
                     NLFIAKPLRGNALVVLDPNLVFLVVDNLFGGDGRFHTRVEGRDFTPTEQRIIQGLLKV
                     TFNEYEKAWSPVQKLDFEYVRSEMNPQFANIATPSEIVVAASFTLEFGGSQAEMHFCF
                     PYSMLEPIRETLYSTMQSDHITQDNRWVGMMGRQLQTAEVELVARLGTAHVTLRDIVG
                     MKPGDVIPLNIPETIQAEVDGIPIFECRYGIQRSQYALKVERFLAQDEDSPPAKAQE"
     gene            complement(58170..58739)
                     /locus_tag="PKX01_01080"
     CDS             complement(58170..58739)
                     /locus_tag="PKX01_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HMZ74740.1"
                     /translation="MSKAPAKAEAAEGEAPAKGGGKKKLIIIVAVVLILAIAGGAAFF
                     LLGKKKHAEGEEEEEPAHHEPPKVKVDPSKPPVFVQLDQFTVNLAPEEGEHFLQATMV
                     LRVADAKVGDSLKAYMPELKHRILMLLSSKRPSELANAEGREELADEVKDEANDVLGY
                     APNPKKKAKRRPEDDGPVLAVLFNQFIVQ"
     gene            complement(58832..59953)
                     /locus_tag="PKX01_01085"
     CDS             complement(58832..59953)
                     /locus_tag="PKX01_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HMZ74741.1"
                     /translation="MPATSLNPIATVAPPAPSGNASASRSGDARTPEASASADGDFGS
                     ALKQQVDRQADDRPRSADEAGSAGSKTRDDTAAAQAPAAPPGEAPPSAEAPQRLGLAL
                     FLGQATGRSEADGRPAARTDDDAPATESPSAALAGLLAPPIDPRPTTPAESAPADTTG
                     KSPAKTAAGLPEFAAAFRTTPTDAGSADAPRLPAGTDAAPANSFAGLHAAALAQIRGE
                     APTPASNALSLPVHTPATHAGWPEEVSNRVSWMVGQHESQAELTLTPPQLGKVEVTVS
                     VSGDQTSAQFVAATPAARDLIEQALPRLRELLGDAGIALGQTSVGTSGQSGNGEGGDG
                     PRRGTAGRGTRGVDGIGSVQGGSAGPWLRRGEGLVDTFA"
     gene            complement(60027..60488)
                     /gene="fliJ"
                     /locus_tag="PKX01_01090"
     CDS             complement(60027..60488)
                     /gene="fliJ"
                     /locus_tag="PKX01_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HMZ74742.1"
                     /translation="MTEPFPLKPLLDLANTRMDEAARRLGELIASEHAVEEKLALLKD
                     YRDEYQARFMEAARNGIGPDAWRNYSAFIARLDDAIAQQQKLASDSRRRTEQGQQAWR
                     DERNKVKAFDTLSQRHHTAQLRKELKQEQRLTDEHAAKHFRDRDADDDGEP"
     gene            complement(60555..61958)
                     /gene="fliI"
                     /locus_tag="PKX01_01095"
     CDS             complement(60555..61958)
                     /gene="fliI"
                     /locus_tag="PKX01_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HMZ74743.1"
                     /translation="MDHGLETWRAFLHDSRKLVNATSPLQLSGRLTRINGLVMEASGL
                     KLPLGSGCRIVVPGGGTVEAEVVGFHGEKIFLMPTDDVFGLAPGAQVLPVEPTQPQLI
                     AGERIEPRRRASDRAKHLPMGDALLGRVVDGAGRPLDGLGPLDVKHTRSLQARPVNPL
                     QRAPISRSLDVGIRAINALLTVGRGQRMGLFAGSGVGKSVLIGMMARYTAAEVIVVGL
                     IGERGREVKEFIEHNLGPEGLARSVVIAAPADTSPLMRLQGASYATTVAEYFRDQGKQ
                     VLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPALVERAGNGPEGGGSITA
                     FYTVLAEGDDQQDPIADSARAILDGHIVLSRNLADQGHYPAIDIEQSISRAMHNLVKP
                     AHFDVVRRFKQLFSRYQRSRDLIAVGAYQAGSDPILDQAVAYYPRLEAFLQQRIEESE
                     PYESAVEKLEALFAGTS"
     gene            complement(61958..62689)
                     /locus_tag="PKX01_01100"
     CDS             complement(61958..62689)
                     /locus_tag="PKX01_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HMZ74744.1"
                     /translation="MALNRHQASGAYRRWDFPSFDAPPPEALAPPPGEPPPDAAVTAT
                     APAPDPSEPPPVEASPAEAALPLPEPDIQLPTAEDIERIHEEARKEGYAAGYEEATAR
                     GRMEAMRLHGLVEGLEAAMSRLDADVAEEVLALSLEVARLMVRQTLATRPESVVDLIR
                     EALVQLPQAHAQIHLNPEDLGMVRDYLGELLTHAGHRLVEDAGVARGGARIEGGGSQV
                     DATVPTRWRRIMDNLGRNIPWEGEG"
     gene            complement(62705..63703)
                     /gene="fliG"
                     /locus_tag="PKX01_01105"
     CDS             complement(62705..63703)
                     /gene="fliG"
                     /locus_tag="PKX01_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HMZ74745.1"
                     /translation="MSSDEGLEKSALLLMTLGADEAAEVLKLLGPREVQKLGAAMAAM
                     PAQSRERMEAILDEVEALAEKGSPIEADHEHIKAMLTKALGDERASHLISRVLQGSDT
                     AGIESLKWMDAPTAADLIKNEHPQIIATILVHLEFDQAGEILKHFTDRLRNDVLLRIA
                     TLDGVQPVALRELNEALTRMLAGASSVKKTAMGGVRHAADILNFVGSAAETAILDNVR
                     EYDPDLAQKILDEMFVFENLLDLDDRGIQTLLREVQSDSLVIALKGAPPEMREKVFKN
                     MSQRAAEMLKEDLESRGPVRLSEVEAEQKEILKTARRLAEEGQIALSSKGGDDAFV"
     gene            complement(63690..65396)
                     /gene="fliF"
                     /locus_tag="PKX01_01110"
     CDS             complement(63690..65396)
                     /gene="fliF"
                     /locus_tag="PKX01_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HMZ74746.1"
                     /translation="MAAEDAAAPPVPPPSPFTQVLENIRALSQRQKIAAGAALAAAIA
                     LVVGVLLWSRQPDYAVLFSNLAEKDGGAVVASLQQQNIPYKFSDNGNAILVPSTQVHD
                     LRLRLAAQGIPKGGLVGFELMETQKLGLSQFHEQVNFQRALEGELSRTISSIAAVAGA
                     RVHLAIPKQTAFLRDEQKPTASVMVNLHQSRSLDPSQVAGIVHLVASSVPNLTNDNVS
                     VIDQDGNLLTKKPDPMRAGLDATQIKYTRELEEGYIQRINDILTPLFGKGNFRAQVTA
                     DVDFNVTEQTAETYRPNPSPEQAIRSQQSSESQTRDAGAQGVPGALTNQPPVPATAPI
                     TTPAVPGTPGANGQLPLSTSKNATINYELDKTIQHTRKALGQIRRLSVAVALNQKEEK
                     DKNGNAKPVPLTEEERKRVEDLVRDAVGYSKDRGDTLSVASSPFVASEKDLPETPLWK
                     DPDNIALFKELLKYLVIAGVIAFVAFGVIRPLMKQVMPPPPEKAEEEASAESAGEAGE
                     EGGGEPGEEGEEGAEVELSPEAAARLAYEEKLARVRDLARSNPRVVANLIKEWMGVNE
                     QR"
     gene            65552..66655
                     /locus_tag="PKX01_01115"
     CDS             65552..66655
                     /locus_tag="PKX01_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ74747.1"
                     /translation="MIPTVAEDRLDARQLAEAFRQFSEASEALTGAYAGLQAQVGQLT
                     ERFSVLMEALPAGVVVLDRAGAVLQVNRVVERMFATVPPGTLWSHFSGSRLVATETPG
                     EWAVACGDGPRRVTLQEAPLESGEGGIVLINDVTEAHRARLAAERTGRLAAMGEMVAG
                     LAHQLRTPLSAALLYTATLAQQDLKPGDRDKIGLRALERLRHLEKLIRDMLLFARGET
                     LGREPFPICDLVAELAHTLEPVARAKGIALAVECDCGRTEVVGDRKAVAGALTNLLEN
                     AVQVLETGGHIRFDAGVEGAHACFRVADDGRGIAPELQSRLFEPFFTTRPDGTGLGLA
                     IARGVARAHGGDIGIASEPGRGTTFTLTLAMGA"
     gene            66694..68076
                     /locus_tag="PKX01_01120"
     CDS             66694..68076
                     /locus_tag="PKX01_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ74748.1"
                     /translation="MNDALHILVVEDDAPLRDAVCLTLEVAGHTVTGVGDGPAALART
                     DDEAFNLVISDLKMQPMDGLELLAELRRRLPQLPVLLMTAFGDVEKAVDAMRGGACDF
                     LMKPFEPQVLLEHVRRYAAPDRDPGGMVAADPHTRKLLALAAKVAETDATVLLTGESG
                     TGKEVFARYLHEQSPRRNGPFVAINCAAIPDTLLEATLFGHEKGAFTGAATAQAGKFE
                     QAAGGTLLLDEISEMPLGLQAKLLRVLQEREVERVGGKKAIALDIRVLATTNRDMTKE
                     VAAGRFREDLYYRLNVFPLAIPSLRERPGDIVPLAHHFARLHGARLRRSARLSPEAES
                     VLVSYHWPGNVRELENTVQRALILSSGESVSGETVRLCLPHWNGEPLRPVLRPATPVP
                     ADFAGEALPGGKELPRPANMKDLEREHILQVLREVGGSRKRAVERLGISERTLRYKLQ
                     QYREEGFFPG"
     gene            68139..68459
                     /gene="fliE"
                     /locus_tag="PKX01_01125"
     CDS             68139..68459
                     /gene="fliE"
                     /locus_tag="PKX01_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HMZ74749.1"
                     /translation="MDTRGVDQMLSELRATAQTAAGKPAQAAAATGQADFGQVLQSAL
                     DQVSSAQMQAQEMAKGFSSEDPNVNLQDVMVNLQKANLSFQQMVQVRNKLVTAYQDIM
                     NMSV"
     gene            complement(68530..69294)
                     /locus_tag="PKX01_01130"
     CDS             complement(68530..69294)
                     /locus_tag="PKX01_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HMZ74750.1"
                     /translation="MPLKFELLQADDYSQYLLREKTDILFNLRGLLQKRAMISAFIDA
                     SADSFLTALLAVTPDERHLILDAAADDSLNRRVEGAEQLICVAQLEKIKIQFAAREIV
                     RFPFEGHEAFRVRMPDVVLRLQRREYYRLTAPISHSLSCLIPVAEGESARPRSVEASV
                     IDISGGGIAVLVPPQGLAFEPEMEFADCRLMLPETGPIVTAIKVRNLFRITHPNGQVM
                     LRAGCEFLGLPGNMSTTIQRYILRTERELNARHRIR"
     gene            complement(69441..70139)
                     /gene="phoU"
                     /locus_tag="PKX01_01135"
     CDS             complement(69441..70139)
                     /gene="phoU"
                     /locus_tag="PKX01_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMZ74751.1"
                     /translation="MNEHTSKKFDAELEGIRTRVLQMGGMVELQIVKAMEGLAHGDLL
                     LIDQTIENDARVNSLEVELDEFCSQIIAKRQPAAVDLRMISTVQKTITDLERIGDEAK
                     KIAKIAKQIHASASPFMPQIRLGHIADAVVDMLRTALDAYARLDTVAAAQVVRQDKDV
                     DAEFKGIMRQLITYMIEDPRTISHAIDMMFVAKSVERIGDHAKNIAEYVVYMVKGRDV
                     RHTSLEEVEREATR"
     gene            complement(70203..70865)
                     /gene="rpiA"
                     /locus_tag="PKX01_01140"
     CDS             complement(70203..70865)
                     /gene="rpiA"
                     /locus_tag="PKX01_01140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HMZ74752.1"
                     /translation="MTQDELKQLAALKALDYVQDGTIVGVGTGSTANFFIDGLAARKE
                     RIVGAVPSSEASAKRLAAHGIRLLDLNDVADIPVYIDGADEIDGTLAMIKGGGGAQTR
                     EKIVAAVAQRFVCICDASKKVAQLGRFPLPVEVIPMARAQVSRELERLGGRPVLREGF
                     VTDNGNLILDVHGLNIHDPVTLEIAIDGIVGVVTSGLFAKRGADVLLLATAQGVQTFE
                     RA"
     gene            71044..71472
                     /locus_tag="PKX01_01145"
     CDS             71044..71472
                     /locus_tag="PKX01_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HMZ74753.1"
                     /translation="MGLLDQLAGQVLGSLGGGQGQGGSDLQHMLLQAVLGLIQNGQGG
                     LGGLVAQLNQAGLGEQVASWVGTGQNLPVSADQISQALGGRLGDMAAQLGLPADQVAG
                     HVAEMLPQVVDHLTPDGQMPQGGDLLSAGLSALGGLFGKA"
     gene            complement(71499..72353)
                     /locus_tag="PKX01_01150"
     CDS             complement(71499..72353)
                     /locus_tag="PKX01_01150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HMZ74754.1"
                     /translation="MNGTFGTLISAAELADQADANWRLFDCRFDLRNRRYGRDAYASG
                     HLPGAVYLDLEEDLSGPLSGLNGRHPLPDPQLLAAKLGSLGVTTGTQVVAYDDAGGMF
                     AARLWWLLRWLGHPTVALLDGGIQAWASSGRALSQDVPALTARVFSPRLQPALHVDAT
                     YVHGHLDDPALCLIDARAPDRFRGENETLDPVGGHIPGAINRFFRDNLGPDGLFKPAA
                     QLQEEFGTLIKGGDAHRVIHQCGSGVTACHNILAMEVAGLTDSRLYAGSWSEWCADPA
                     RPVATGPG"
     gene            complement(72366..72728)
                     /locus_tag="PKX01_01155"
     CDS             complement(72366..72728)
                     /locus_tag="PKX01_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HMZ74755.1"
                     /translation="MITITHDAGQVDAVVFGEFTLSDYKEFEEVFNYTVRFEGPVNLF
                     FDLRAMSGFTVDMALEEIRFARAHANDFAQIAVVTSDQWVSWSAWLSQLFVNADVRVF
                     DDADDARAWLAGVESPVQ"
     gene            complement(72801..73664)
                     /locus_tag="PKX01_01160"
     CDS             complement(72801..73664)
                     /locus_tag="PKX01_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ74756.1"
                     /translation="MQSLWMIAASFLFACMGVCVKLGALRFSTGELVFYRGLIGLLMM
                     AVLLRVQGIPFATPHWKLQLSRGLSGTVALMCYFFALGILPLATAVTLNYTSPIFVAL
                     ILALWFREPVGPLRHAAVALGFVGVVLLLKPTLQPDQWPGALGGLGSGLIASLAYINV
                     RELGRAGEPEARTVLWFSLITAAFGLPWALGSDHLHRLDASGLAIVLGVGGFGGCAQL
                     AMTRAYRYGKTIVSANLAYTTVIFSSAFGALLWDERLPWTSFIAIALIVASGVLVSLA
                     SRKPAPVAESD"
     gene            complement(73829..>74973)
                     /gene="aceA"
                     /locus_tag="PKX01_01165"
     CDS             complement(73829..>74973)
                     /gene="aceA"
                     /locus_tag="PKX01_01165"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HMZ74757.1"
                     /translation="KVNGAAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADG
                     NTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWSRGVNPGDEGFIDYFLPIVAD
                     AEAGFGGVLNAFELMKNMIAAGAAGVHFEDQLAAAKKCGHMGGKVLVPTREAIEKLIS
                     ARFAADVMGVPTLILARTDAEAANLITSDYDENDKPFLTGERTQEGFFRVKNGLEQSI
                     SRGVAYAPYADLVWCETGVPDIGFAREFAQAIQAACPGKLLSYNCSPSFNWKKNLNDS
                     QIASFQEELSALGYKYQFITLAGIHVNWYNTFQFAHAYARGEGMKHYVEMVQEPEFAA
                     REKGYTFVSHQQEVGTGYFDDVTTVIQGGSSSVKALTGSTEEEQFH"
BASE COUNT        11263 a        23463 c        27588 g        12659 t
ORIGIN      
        1 cgctgcgcat ttttcaatcg actgagcttg atcgttaggc atcgcattaa ggatccgtat
       61 ctatcatgtt caaagtgttc gtcagcccag taggtcgggt tagcgcagcg taacccgaca
      121 ctttccatgc agtgaattca gacgggtcga cctttccccg cagcgcttga tccggttgta
      181 gcttcatccg ggcgctcaaa taaaaacggg ccggacagtc ccctgtccgg cccgtttccc
      241 atacatcacg cctacatcaa tcaacccttg cgacgcccca ccatgcatcc catcagcccc
      301 agcgcggcga gggcgagggt ggtgggttcg gggatgcgtt gggcctggta gctgggcagg
      361 gtgtcgtcgg tgacgccgaa gacgaagacg cggttggggt tggcgatgcc gccgacggtg
      421 ctcaggaagt cgttgtcgtt ggcgacgatg agggtgtgct ccacgacgcc gctgacgagg
      481 acgtccggac cgaaggcgac gccttcgagc ttggcgggga tggtggcggt ggtgtagccg
      541 acgctggtga gggcggcgac gacgtcgagg aagagggttt tgccgacggc cttgggggcg
      601 aggttggcgg cgccgctgag ggcgctgacg tcggcggcgc cgctcaggtc gatcttgtag
      661 agctttttga cggcggcgtt ggagccgtcg ccgaggccct tgccgtcgcg ctcgtccacc
      721 aggaattcgt ggtcgttgat ggcgacgatt tcgctgacgg tggggtactt gggcttggcg
      781 acggtgccga tgttgtcgag cttgtaggcg tattcgtggg tgactttgcc ggtggcgatg
      841 tcgatggtga cgatgcgcac ggtgctgccg tcggtgccgc cgtcctggat gagggggctt
      901 tgcatgatgc cgacgagggt cttgccgtcc ggggtgatgg cgaggccttc catgcccttg
      961 ttggcgacgc gaccgctggt gttgccggag atttcggtgg cgccctggct gctcaggttg
     1021 gaaacggcga acttggacgg gagggtgatg acgccgatgc gttcgccggt ggcgcggtcg
     1081 aactggtaga tgtaggggcc gtactcgtcg gagacgaaga cgctcttgcc gttctgggcg
     1141 acgcggatgc tttccgggtc gaggcgggcg ttgttggggt tgctggagag gccggcgccg
     1201 aaggcgtcgg agcggccggt gaagtagttg acgccgctgc tgttgagggc cggggcgctg
     1261 ccgtagttga ggggcgtggc gctggacagc agggtggtgt cggtgagggt gggcgtcagg
     1321 gtggtgacgc tgccggaggt gaagacggcg ctgctgaggc cgagattgac ggtctggaag
     1381 cgggagacgt aggaggtggt gtcgtccacg gcgctgttcc aggcggtggc gttggggccg
     1441 cggtccgggg tgaacaggaa ggtgtcgccg ccggcccagg cgacgccgga gccgatgccg
     1501 cccagcgtgg aggcggaggc gccgctttcc agcgcgccgg tgaggccgga catgtcgggg
     1561 cccgagaggg tgccggtgcc gatgagggtg acgcccgcgt gggcggaggt ggcgagcacg
     1621 gccggcacga gggcggccaa cagtcggcgg gtgaacatgg cgactcctga tggaaaaagg
     1681 cgtcagccta cgcaggccat gttgcgcaga aattaacgtt tttgcggtgc agggcaggag
     1741 aaggcgctgg gcgtgttggc gtgctgcgga gaatgtgcgc cggggcggtg gtgcgtgccg
     1801 cgggtgggcg cggcacccgt gtccgggcgg cgcagcgttg ggcggacgcg cgaaggcccg
     1861 cggaatggtg cgcgaagggg gtacagatgg acacacgccc ggccgagact gcggccgggc
     1921 gtggtgagac gggggcgcgg cttacttgtt gccgtccacg tccttgggca ggccggtctt
     1981 cttcacctcg ccgacgcggc ggccggtgag ggtgacggtc tggcgcacgg gctcttcgag
     2041 cagggcgggc gtgccgcggt tggggttgcc gcgggggccg ttcttgccga agccggaggc
     2101 gcggtagttt tcgccgctga tgcgccggtt gagggcggct tcggggtcgc gatagttggt
     2161 cttggcgcgg ggctggtagt ccacgcggtt gccgaagtcg tcgtccacat tgccgccttc
     2221 gcgggggcgc ggggccttgt tgggattggg gccgccatga gcgccgccat gggcgccgtg
     2281 cttcttgccc tggggggcgg acggggcctt gtggccgggc tggctgccct gtcctccctg
     2341 gccgccgtgg ccgtgcttgc cctgacgttg ttccttgccg tgctggggtt tgccctggcc
     2401 gttgccctgc ccctgttgtc cttgcccttg accctggctc tgacccttgc cctgctgcgg
     2461 gggcttgccg tgtctgggct tgccggcctt ctggccctgg ccttgcccgc cggtctggcc
     2521 gtgttgcggt ttgccttcgc cactggcggc cttggcgtcg ccctgggggg cggcggcgcg
     2581 ggcatgttcg ctgcgcggtt tgtcgtgacg gccgcggctg ccggtgtggg tgccacgggg
     2641 ttcgcggggc gggcgggtgt cgccttcgtc gggggtggcg gagggcacga agtcgggcac
     2701 ctcgaccttg tcgatgaggc gcttcatggt gcgctcgatg tccttgagga ggggcagttc
     2761 ctcctgatcc accagggaca cggcggcgcc ttcggccccg gcgcggccgg tgcggccgat
     2821 gcggtggacg tagtcttcgg gcacattggg aagttcgaag ttcaccacct ggggcagctg
     2881 gtcgatgtcc aggccgcggg cggcgatgtc ggtggcgacg aggacctgga gcttggcgtc
     2941 cttgaagtcg gcgagggcgc gggtgcgcgc ggactggctc ttgttgccgt ggatggcggc
     3001 ggaggggatg ccgtgcttgc ccaggtattc ggcaagcttg ttggcgccgt gcttggtgcg
     3061 ggtgaagacc agcacctgga accactggtg cttctggatg aggaaggcga ggagttcgcg
     3121 cttctgcttt tgcgggcaca ggtgcacgga ctggcgcacc agctcggagg cggtgttgcg
     3181 ccgcgccacc tcgacgaagc cggggttgtg caggagaccg tcggcgaggg ccttgatctc
     3241 gtcagagaag gtggccgaga agagcaggtt ctggcgctgc ttgggcagca tcgcgaggat
     3301 cttgcggatg tcgcggatga agcccatgtc gagcatgcgg tcggcttcgt cgaggaccag
     3361 gatctcgacg ccggagaggt ccacggtctt ttgtgtgcag tggtcgagga gccggccggg
     3421 cgtggcgacg aggatgtcca cgcgcttctt gagcttggtg atctgcggat tgatggcgac
     3481 gccgccgaac atcaccatgg aggtgagggg caggtgcttg ccgtaggtct gtacggattc
     3541 ctcgacctgg gcggcgagtt cccgcgtggg ggtgaggatc aggcaacgtg gtcggccggt
     3601 ctggacgttg tgggcgtggg cctcggacag gcgatgcagg atcggcagcg tgaagccggc
     3661 ggtcttgccg gtgccggtct gggcggcggc catgaggtcg ccgccggcga ggacctgggg
     3721 gatcgcctgg acctggatcg gggtgggaac ggtgtagccg gtatcggcaa tggcgcgctg
     3781 gatgggctct gagagggcca ggtcagcaaa gctgaccgcc agcggcgcct gcacggcgga
     3841 ctggggctgg gtcatggaat tgtgtgctcc tgcgacggcc tgaacgcccg gggagggcga
     3901 taccaatcgg ggcggacgct gcggttttcg gggtctggaa cccaccggat caacggatca
     3961 aggccggtgc gctgattggc cgggcgcacg gttgccgaat tgtacggaat tacgcggagt
     4021 tttgcgtgga ttttgtcgtg tttggtgttc gacgcggtgc gcggagtgtg gcggccgcgc
     4081 cgcattcaat cgtctttggc gccggggatg ctggccatgg cggtgcggaa ttcgcccagg
     4141 gcggcgtcga gggcgtcgag atcgccctgc agcgcgtccg gcggctgctg ggcgcgcagg
     4201 cggtcgttga ggcgcgtggc caggtcgcgg atgcgctcgg cgcccaggtt gccggcggcg
     4261 cccttgaggc tgtgggcgat gcggccggcg tcgctgctgc gtccggcggc gagggcatcg
     4321 cgcagggcgg tggcgtcgtg gccgtggtgc tccaccagca tgtggagcag ccgtgcgtag
     4381 cgccggacgt tgccgcgcac gacctgcagg ccgaagtcca ggtccatgcc gggcagggtg
     4441 ctcaggtcgg ggagcggggc ttcgacgggc ggttcggtcg cagggggcgc ggtggtggtc
     4501 gttgggctgc ctggggcggc gttgagccag gcgtcgaggg tggcaatcag cgcgtccgga
     4561 tcgacgggct tggcgaggtg gtcattcatt ccggcggcca ggcagtcctg gcggtccttt
     4621 ccgaaagcgt tggcggtcat ggcgatgatg ggaatgtcgc ggtaccccgg caggctgcgg
     4681 atgcggcggg tggcttccag tccgtccatc tccggcatct gcatgtccat caggatggcg
     4741 tcatacctga gggccgcggt cttcccgacg gcgtcgatgc cgtctaccgc tagatcggca
     4801 cgatagcccg catccttgag gagtgtcagg gcaacttcct gattgatggg gttgtcctcg
     4861 cacacgagga tgcgcgtctc gcccggggga cgtcgggtgg tggcgtgggg gcggggcggg
     4921 gcgacgggct cggtggcggg cgcgccgcgg cggaagcggg cggtgagcca gaaggtgctg
     4981 ccggtgccat gggcgctggt gacgccgacc tcgccgccca tcaggtgggc caggcggcgg
     5041 gtgatggcga ggccgaggcc ggtgccgccg tagcgccggg tggtggagcc gtcggcctgc
     5101 tcgaaggcgt tgaagaggcg gtcgagggcg tcggcgcgga tgccgatccc ggtgtcctcg
     5161 acttcgaagc gcacctgcac attgtcggca tcggcatctt cgacccgggc gcgcaggacg
     5221 atgcgtccgc gctcggtgaa cttgaccgcg ttgctggcgt agttgaggag ggcctgggtg
     5281 aggcgtgtgg ggtcgccgcg cagggggccg ggcagcgggt cgggcagttc cacatagaga
     5341 ttgacctgac ggctgcgggc gcggtcgcgg accagatcag ccacccggtg cagcacggtg
     5401 gccagatcca ggtcggtctc ctcgatctcc agcttgccgg cttcgatctt ggagatgtcg
     5461 aggatgtcgt cgatgaccga cagcaggtgc tgggcggcgg cgatgatctt gccgaggcgg
     5521 tcatcctgct ccggcgtggt ggggccgcgc cgcagcaggt gggtcatgcc caggatggcg
     5581 ttcatcggcg tgcggatctc gtggctcatg ttggccagga aggcgctctt ggcctggttg
     5641 gccacttcgg ccgcgtggcg ggcctcttcg agctgggcgg tgcgctggtg gacgagctct
     5701 tccagatggt ggcggtggcg gtcgagctcc tcgccgatgc gcttctgggc ggtgatgttc
     5761 tccttgaccc acacgtagtg ggtgatgcgt ccgtccgcct gacgcatggg ggcgatctgg
     5821 gcgtattcgt agaagatgtc gccgttcttg tggcggttga tgaactggcc gccccagatg
     5881 cggccttccc gcagggcgct ccacatgctg gtgcgcacat ccttgagggg aatggcgtcc
     5941 tggagctgct ggacgtcgca gccgagggcc tcgtcgcggc tgtagccggc gttgcggacg
     6001 aaggcctcgt tgacgtattc gatgcgcccg gccaggtcgg tgatgacgat gctggtggga
     6061 ctctgttcga ccgcgaggga cagcttgcgc aattggtcct cggcctgccg ccgcccggtg
     6121 atgtcgttgg cctggacgag gagggcaacg aggctgccgt gggcgttgcg gatcgcggtg
     6181 gtggcggtat ccgcgtgacg gaagctgcca tcggccgcct ggaagacgtc ctcggtgagg
     6241 agcggacctt cctcgccatg ctggaagcgg gccaggcgtt cgggccagat gctgtgccac
     6301 tcgggtagct gccgccaggc cggcgcgctc cagaagggct tgcccaggta gctggcgcgg
     6361 gtgccgccga gcacgtgcag gataagttcg ttgacctcca gcaccgtgcc gtccggggcg
     6421 agcagggcca tgagctggaa ctgctggttg aaggcggcgc ggaagcggct ttcgctgtcg
     6481 cgcagggcct gctcaccggc gcgcaggtct tccaccaggc tgaggaggac gctgcgggac
     6541 tgctcggctt cgagaagatg gcgctgggcg gcctggcagg cttcctccag gtggcgctcg
     6601 gcctttttgc gttcggtgac gtctcgggcc acgccgagga cgccgatgag gctgccgtcg
     6661 gcggcgaaca tgggcgtgcg ggtgatctcg tgggcgacct gcgcgccggt ggcccggtgg
     6721 atgctccagc gctcggtggc ggtcggggtg ccggcggcga cggccgcggc gtcgtcgtcg
     6781 cggacgcggg cggcatgttc ggcgccgacg aaatcggcgt cgctgcgacc aaggatgtcg
     6841 gcttcgggag cgttgaagta agtctggaac gcccggttgc aggccaggta gatgccatcc
     6901 ggatccttca gccagaccag atcgggaatg gtgtgcagga gcgtctgcag gcgcagggat
     6961 tcggcgtctg cagaggtccg ggcgtctgcg tcaactttgc cgcgccggcg ccgcagcgcg
     7021 acggtcgcgg cgacggcggc cagggcgccg acggcgatcc aggccagggc cgacagtgcg
     7081 ggtgcggacg atgcggcgtc catcgcgggg gccgcgaggg gccaggcagc cagcagaacg
     7141 gcgagcggcc gctgcggcaa gcgcaggcgc gtcacgccgg gcgcccctcg gacggttgat
     7201 cggcgctggg gtagcggatc ggctgaccgg tggcgcgcag cagggcgttc acttcgcgct
     7261 tgagctcgag cacgcggcct tcgcggccga gcatggcgtc gtaccagcgg ttgagttcgg
     7321 cgaggcgcag ggacagctgc tcctgcacgg acttgcgttc ggtgacgtcc acgaaggtgg
     7381 cgaggaaatc gtcgccgagc acgatgcccg aggcgtccac gatgcgcacc gagccgtcct
     7441 tgcagacgac gcgaaattcc tgcggggcga tggcctggcc gtggcgtcgg gcggtcagga
     7501 aggcttcgcg ccaggcggcg tcggcgcggg cacgcaggtc ccggtcgggg aaggcccgct
     7561 ggcgccaggc gtcgctgtgg gggaggtcgt cgatggtgta gccgaagatc tgctggaagt
     7621 ggtcgttgag gaacaggcag cgtccgcccc gggcgatgtg catgacgggg atcggcaagc
     7681 gctccatgag ccgccggaag cggtgctcgg attcggtggc gcggctggtg gcggcctttt
     7741 cgcccgagat gtcgatggtg atgcccgccg cgccgagcgc cagtccgtcg tcgtcccggt
     7801 ccacgttgcg gccgatcgtg cgcagccata gccagctgcc gtcgggccgc agcacccggt
     7861 actcgtacga gaagggctcg tcattgccgt tgcgctgctc gcgggcggcc tcgatgatgg
     7921 tgcgcaggcg tgggaggtcg tcggggtgaa cgcgggcaaa ggccgcatcc agggtttcgt
     7981 cacggccgga gggcgcgtcc acgcccagca gcttccacag ggcggcgttg cgggtgaggc
     8041 gtccggcggc gtagtcgtat tcccacgtgc cgacgccggt ggcgtcgagg atcacgtcca
     8101 ggcgatcctg caggcgggaa cgcatctcgt ccaggagggt cgcgccgagg cgttccagca
     8161 tgcggtgctg gctgaccacg ccggccaggc gatcctgttc gtcgagggcc accatgtgcc
     8221 ggatgcccgc ggcatccatg cggtcggtgg cggtggccag ggtgtcgtgg gtgcggatgg
     8281 actgcaccgg cgaggacatg acctgatcga gcgcggtggt ggacgggttc acgccgcgca
     8341 gcatgaggcg cggcacgtcg cgttcggtga ggatgccgac ggggctgccc tggcgggtga
     8401 cgaccacgtg gtcgcagcgg cccgtgctca tgcagtcgag ggcgcgttgc agcggggcgt
     8461 cgggtggcag ggtggtgaac tccgggtcca tcaccgattc gatgctgcgg atgcgcgaga
     8521 agaggtcccg gttcaggtgg gcccggaagt cgctctcggt gacgattccc cacagctggc
     8581 ccgcgtcgtc ggtgaccacg aggtgacgga tgccctggcg cgcgaggaga ttctgcgcgg
     8641 ccggaaagtc caggtccatg cgggccgtga gcagcggctg gctcatgacg tcgccgatcg
     8701 ccgtggcggg ccggaggccg ttgctcatga cgcgcacgat gtcccgttcc gtgaggatgc
     8761 cgaccgggcg gagaccttcg gtgacgacca cacaggagac gtgctgctgg gccatcaggg
     8821 cgatggcgtc gcgtagaggg ctgtccggaa gaacggtgcg aaccgacgtg gtggcgatgt
     8881 cgctgaggcg caggtagcgg tgacgctgca tggcggacgg gagcgggaaa agggccagag
     8941 tgcggagtgt acccccgagg cgcggcggcg tctcgtctgt gggcgcgggg tgacgatccg
     9001 tcaccgtaca atggcgcctt catgcctgcc ggtcctcctg tctccgtgtc ctcgtccgtc
     9061 ctgaccctgc ccagtccctt ctgccccagc gaccggatcg gcctgctgga gtcaccggag
     9121 tgcgaccggt ccgccgtatt gcgacgggtc gagacggggg actacgacaa gcccttcgtc
     9181 ctcgacgacg gcacgacccg cagcctgcat ttctcgctgg ccctgatcca gagcaccatg
     9241 agcctgaagg atcccttcgc gcttgagctg gactacacgc gggcgatgat gagtttcctg
     9301 ctcttcctgc cacgtccgcg gcatttgctg atgctggggc tggggggcgg ctcgctggtc
     9361 aagtactgcc atcgccatct ggggtcggcg cggctcacgg tggtggagat cgacccccat
     9421 gtgatcgcat tccgggacga attcgaagtg ccgccggacg atgggcgcct gcaggtgatc
     9481 cagggcgacg gggcggcctg ggtggcggac tgttccgagc ggccggacgt gatcctgctg
     9541 gacgccttcg accgggacgg gctgtccggc tcggtgagca acgagacgtt ctacgggcag
     9601 gtctgcgcgg ccctcgccgg gcgtggctgg ctggtggcca acctggcggg cgacaaggcg
     9661 gagcgggtcg cccaccggga catgatcgcg gcggccttcg gcgagcgggt gtggtcactg
     9721 acggtgcccg acgggggcaa cgacatcgtg ctcgccgccc gggccgaggg ctttgcgccg
     9781 cgctggcgcg agctggccaa tcaggccagg gcgctacgtg cccgtctggg actggatttt
     9841 ccggacttcg tgcggcgtct ggagcgcagc cagcgcctgc gctggaccta gaccggttca
     9901 ggcgtcggcg agcaatccgt ccggatgggt gtagatcacc aggcgacggt cgcgggcaaa
     9961 gccggcgagg gtcacgcccg cggcgtcggc caggcggatc gctagggcgg tgggcgcgga
    10021 gaccgctacg aggctcccga tgcccactgt ggccaccttc tgcaccatct cgtagctggc
    10081 gcgactggag acgagcaccc agccggcgtg cgggtcggta gcgctgcggg ccagatgacc
    10141 cagcagcttg tcgagggcgt tgtgtcggcc cacgtcttcc cgcagggcga ggagcgtgcc
    10201 ccggcggtcg atccaggcgg cggcatgcac ggcgccggtg gtggcgcgca ggggctgatg
    10261 ggccggcagc gcggccaggg cggcggcgat ggcggaggcg ggtatcggcg cgccgcgcgt
    10321 caccgggccc ggtgcgcggg cgagggcggc caggctctcg accccgcaca gcccgcagcc
    10381 cgtgcgcccc gccagactgc ggcgacgggc cttgaggtcg gcaaaacatc cggcggcgac
    10441 atgcaggcgc agttcgagcc cgtgcggcgt ggcgacggtc tcgctgtcga aaagctccgt
    10501 cggcgtggcg aggatgccct cggagaggct gaagcccagg gccagatcct ccagatcggc
    10561 cggcgtggcg aggagcacgg cgtgggagat gccgttgtag accagcgcca ccggcacctc
    10621 ttcggcgacg gtgtcctctg tggcccgcag ctgcgggccg tcgagacgcc gaacggtcag
    10681 cgtggtggtg gcctgcggcg cgggcggctc gtcaggcggg cattcgtcgg gcgggagagg
    10741 cggcatggcg ggcggcgcag aggagggtgg cgccgattgt agccgcagcg ccagaccgtc
    10801 gcggcggtcg gggcgtcgtc cggcggggat gttcgccgtg ccggtgcctg gcacccttgg
    10861 cgaggcgcgc tcaccgtggg gagcagggcc tccggatgct gtccgcgggc ccggggcgct
    10921 ggcgggcagg atgccgcgtc agcacaggcc tgcggcagag ggtgcggcag gcccatgaaa
    10981 acggggcacc ccgcagggtg ccccgtccat gtgccgggag gcgtgatgcg atcagaagcg
    11041 gtagccgacg ccgacgcccc aggtgacggg gttcaggtcg agcttgccaa tcttgctgcc
    11101 gcccagcttc acgtcggtgt ccatctggat gtacttggcg tccacgttga gggaccagtt
    11161 cttgtccagc ttgtagtcgg cgcccacctg ggcggcgaag ccgaagctgg cgcgatccac
    11221 ggacacggtg ccgagggcgg tgtcaaagct cttcttggtg aagatggtgt agttcacgcc
    11281 gaggccaacg tagggcttga aggcgcccag ttcggtgaag tggtactgca ccagcaggga
    11341 cggcggcagg gcctggacct tgccgatctt gcccagggcg gaggtctgga tgtccacgtc
    11401 ctgcgggtag gtgaggacca gttcggcggc gatgttcggg gtgaagaagt agctgatgtc
    11461 cacttccggg atccagcgct tcttggcgct cacgtcgccc acgccggcgt tctgcaggtt
    11521 gtcggcgttc ttgttgtccc agtcgagatt gaccgcgcgg gcgcggacca tccacgggct
    11581 ttcggcatga gcgagcccgg cggacagggc ggcaatggcg gcgatggcgg aaacaacgat
    11641 cttcttgtgc atgggtgact ccgcctcttc ctgaatgtga cggaaagatt acagccgggc
    11701 cgatcctacg cctcggtgcc tggcggatct tgacggaggt caagggatat ccatttttgc
    11761 agtgtggtct ttggttcggg gtgctgccgt gtgtccgcat caaaaaaaac ggcgaccgga
    11821 tcgctccggt cgccgtcaag gcaacgtggg gcagagcccc gtcctggtga aacccggtgc
    11881 ttacggaatc attccggtga acagataggc ctgggcggtg gtgatgatgc ccaccatcgt
    11941 ggcgaacagc aggctgtgct tgaccgtgaa gcggaacagg tccgattcct tgcccaccag
    12001 acccacggcg gcgcaggcca cggcgatgga ctggggcgag atcatcttgc cggtgacgcc
    12061 gccggtggtg ttggccgcca ccagcagggt gtcggacacg ccgacctggt gggcggtggt
    12121 ggcctgcagg gagcagaaca gggcgttgga cgaggtgtcc gacccggtca ggaacacgcc
    12181 gagccagccc aggaacggcg agaagaacgg gaacagcttg ccggtgccgg ccagcagcag
    12241 ggccagggtg gcggacaggc cggagtagtt ggccacgaag gcgaaggcca gcaccatgcc
    12301 gatggagtag atcgggcgct tgagctccag caccgtctcc ttgaaggtct tgaccgcctc
    12361 gccgccgcgc aggcccagca cgagcatgct gatcagcgct gacaggaaga tggcggtgcc
    12421 ggtggcggag agcaggtcca acttgtacac cgccgcatag ggcgtggcct tggcgacgat
    12481 gggttccatc ttgatgacca gcttgtcgag catgggcacg ggccactgga gcaccaggct
    12541 gtacaggggg ccgtccttgg cgaagagggc cttgaagggc ttgaggctcc agatcgtcac
    12601 catcacggtg aggatcacga agggcgacca ggccttggcg atctggccga ggctgtagtg
    12661 ctggtcctcg cccagttcgg cgtggtgctg ggtgtcgaag cggaagatgt tcttcggctt
    12721 ccagttcttc aggaagacgg tcaggctcac caggctcacc agggcggagg tgatgtccgg
    12781 cagttccggt ccgatgtagt tggcggtgaa gaactgggtg acggcgaagg tgccgccggc
    12841 cacgaggatg gcgggccagg tctccttgat gccgcgccag ccgtccatca tcgccaccag
    12901 ccagaaaggc acgatgaccg acagcagcgg cagctggcgg ccggccatct ggccgatctt
    12961 gaaggggtcg atgccggtca cctggccacc cacgatgatg gggatgccca tggcgccgaa
    13021 ggccaccggc gcggtgttgg cgatcaggca cagaccggcg gcgtagaggg ggttgaagcc
    13081 caggccgacg agcagcgcag cggtgatcgc caccggcgca ccgaagcccg ccgcgccttc
    13141 caggaaggcg ccgaaggaga agcccaccag cagcatctgc aggcgctggt catcggtgat
    13201 ggagatgacc gaggcgcgga tgatgtcgaa ctggccggtc ttgacggtga tcttgtagag
    13261 gaacaccgcc gcgatgatga tccacgcaat gggccacagg ccgtacagga agccgtagcc
    13321 ggcggaggcg aaagccatct ccacaggcat gttgtagaag aagatggcca ccgcgagggc
    13381 gatgccgacg gtgatcgtgc cggcgatgtg gcccttcagg cgcagcaccg cgagggcgat
    13441 gaagaagaag atgatcggca gtgcggcgac cagggccgac aaccagaggt tgccggctgg
    13501 atcgtagatc tgcatccagg tttgcatggt gtgtttcctc cttgggtgaa tgcgggtcga
    13561 aagtcgggtc gggggcgggc ggggttcagc aggtggcgat acgggccgcc tcggcgctgc
    13621 attcgtccag cagatggacg atggagcggt agggcaggcc ggtggtgtcg gtgaggccga
    13681 tctcgcaggt ggcgttgctg gagtagccgc cgcagcagcc ggccggcacc tccagcttgc
    13741 gcagggcatg ggcgttgagt tccggcacga cgaagccccg gtcgccgccg aagccgcagc
    13801 acttcacctc gggcggcagc acgggctgct ccgcgcaggc ggcggcgagg gccttgagtt
    13861 gggtctcgaa gcccatgcgc cgggccgagc agttgaggtg gatcagcacc gggtcggcct
    13921 tgcgttgcag catcaggcgc ggcagcagcg cgtcatgggc gaactccacc agatccagga
    13981 ccggcagccg cccggcgagg aaggtcttca tgcgcaggga gcaggcgctg gcgtcgagga
    14041 cgatggggat cgcgccgtcg cggctggcgg cacgcagcac ggcttccagc tcggcagact
    14101 tgcggtcggc ctcgtcggcg aggccgcggc tggcgaagga ctggccacag cacagggcat
    14161 cgacgccgtc cggcacgatg ggggcgtagc cagcccgctc cattactcgg atgacggtgg
    14221 cggagagggc ctgctccggc gcgtcggcgc cgaagatgcg gcctgcacag gtggggaaat
    14281 aaaccaccgg gtcgcccgcc gagcgggacg cgcggggggc gggctgggga tggggcatgc
    14341 cgcgcttcca ggcgccgccg ctgagacgcg cgagggcgcg ggtgccgatc acggattccg
    14401 ccagatgacc ggccttgagg gcggtgcggg cgagggcctg ggtggcgccg aagtgttccg
    14461 ccgccaggcg ccccaggcgc cgggccacgc cggagagccg ctcgccacgg accgcgcggg
    14521 tcagggcgcc ggtctcgatg cccaccgggc aggcggtgga acacaggccg caggcggcgc
    14581 aggtgtccag gcccatgtag gtgtaatccg cgtcgagggt gtccggcgcc tcgccggcgg
    14641 ctgcgcgccg ggacagctcg cgccaggcga cgatgcgctg acgcggcgac agggtcaggc
    14701 cgtgggacgg gcactggggc tcgcagaagc cgcactcgat gcagcggtcc accggcgcgt
    14761 agagctggcc ggcggcgggc atgggcttga ggtgcttgag gtgggcctcg gcgtcgtcgt
    14821 tgatgatcac gccggggttg agcaggcctt gcggatcgaa gagctgcttg atggccttca
    14881 tcagggcgta ggcttcgctg ccccattcca gctcgacgaa gggcgccatg ttgcgtccgg
    14941 tgccgtgctc ggccttgagg gaggcgtcgt acttcagcac gagcacggtg cacagctcgt
    15001 ccatgaaggc ggcgtagcgg gccacttcgg attcgatgcc gaagtcctgg gtgaacacga
    15061 agtggacgtt cccctcgagg gcgtggccga agatgatggc ttcgtggtag ccatgggcgg
    15121 cgaacaggcg ctgcaggtcg aggcagcagg ccgccagatc ctccacggcg acggccacgt
    15181 cttcgatgat gacggtggtg cccggcttgc ggacggcgcc gaccgccggg aaggtgccct
    15241 tgcgcacgtt ccacaggcgc tcgatctcgg ccggcgcggt ggtgaaggcg gcgggctcgg
    15301 tgagcgggtg gcaggccagt tcggccagta cggcggcgat gcgggcgtcg agggtgacgg
    15361 catcgggggc gcgggtttcg atgaggaggg cggtggcgcc gtcggcgagg gtctggagca
    15421 ggggcggcat gccgggtttg tcggcgaccg cccgcaggga ggcccggtcg aggagctcca
    15481 ccgcgtccac cggctgctgc ctgagaccga ccaccgcccg gcaggcgctc tccatgtccg
    15541 ggaagaacac cagggcgctg gccttgtggg cgacctcggg cacggtgtgg taacggactt
    15601 cggagaggaa gccgagggtg ccttccgagc cgatcatgag gtgggcgagg atgtcgatcg
    15661 ggtctgcgaa atccaccagg gcgttgaggc tgtagccggt ggtgttcttg atcttgtact
    15721 tggcgcggat gcgcgtggcg agggcggggc ggccctgcac cgcggtggcc agctcggcga
    15781 ggccttgcag cagggcgccg tggctcttct ggaaggcggc ccggctggcg ctgtcgccgg
    15841 tgtccagcac gctgccgtcg gcgaacatca cgcgcaggct ggcgagggtg cggtagctgt
    15901 tctgggcggt gccgcagcac atgccggaac tgttgttggc ggcgatcccg ccgatcttgg
    15961 cggcgttgat ggaggccggg tcggggccga tcttgcggcc gaagggggcg aggcggcggt
    16021 tgacctcggc gccgacgatg gcgggtccga cggccacgtg ggcgccgtcg tcggcgatgc
    16081 ggcaggcggc aaagccttcg cccagcagca ccagcacgcc atcggtgacg gcctggccgc
    16141 acaggctggt gccggcggcg cggaaggtga cgctgcggcc gtgctggcgg gcgaggtcga
    16201 gcacgtcgcg gacttcggct tcgtcgtgca cgacggtgac gacttcggga atcaggcggt
    16261 agaagctgcc gtcggtgcca taggccaggc ggcgcagttc gtcggtgatc acgcggctgg
    16321 cgggcagtcg ctggcgcagg gcatcgatga gcgcggacac ggtggttcct cccgtgaggg
    16381 atgaggtgga ttcggtaggg tggcgggggc ggtcgtcgga cgcgccgggg cggggccgca
    16441 ggcggccccg acgggctgtc cgctatctgc ggtcgcgcag catgtcccgg agccttcgtg
    16501 gagcgggttg caggggcagc cgggcagcgg tccagcccgc ctggcgggtc ggcgtcaggg
    16561 cgcggaagcg gctcgccagc cagctgaagg tccggtagcc cagcgggctg gcgtacaccc
    16621 gtgcccaggc gcgccaggcg aagctgacga ggcggtcgtg gccggcaccc tggcccgcca
    16681 tcacggggtt tgcctgcggt gcggactggg cctcgccccg caggcggatg agcagctcgg
    16741 ggatggggat cttcaccggg cagacttcgc cgcaggcgcc gcacaggctg gagaggctgg
    16801 gcatcagatg ggtctgttgg agccccatca tgtggggcga gacgatcttg ccgatgggac
    16861 cggggtaggt ggtgccatag gcgtgaccgc cgatccgggt gtagaccggg cagtggttca
    16921 tgcaggcgcc gcagcggatg cactggaggg tcttgcgcag ctgggcgtcg gcgtaggcct
    16981 gggagcggcc gttgtcgagg aggaccaggt gcacctcttc ggggccgtcc cgttcgtcgg
    17041 gcttgcgcgg gccggagatc aggttgaaat aggtggagac ggactggccg gtggcggagc
    17101 gggtcaggat cgaaaacagc ggcgccacgt gctccagctt ctcgacgacc ttctcgatgc
    17161 cggtgatggc cacatgcacc cgcggcacgg tggtgcacat gcgcccgttg ccttcgttct
    17221 ccacgaggca cagggtgccg gtctcggcca ccgcgaaatt gacgccggag aggccgatgt
    17281 cggcagtctc gaacttctcc cgcagcaccc ggcggccgat ctggatgagg gcgtccacgt
    17341 tgtcggtgta atcgacgccg gggatcttgt cggcgaacag gcgggcgatc tgctgcttgg
    17401 tctggtggat cgccggcatg atgatgtggg agggcttctc gccggccagc tggacgatgt
    17461 attcgcccat gtccgattcc agcgcgtcga tgccggcggc gtgcatggcg tggttgaact
    17521 cgatctcctc gctcaccatg gacttgccct tgacgatcag ctgcgcggca tggcgccggg
    17581 cgatgtcgag gagaatggcg ttggcctcgt cgggcgtttc ggcccaatgg acgtgcacgc
    17641 cattggcggt gaggcgcgct tcgagctgtt ccagcaggtc gggcagcctg gacaggctgt
    17701 actggcggat ggactcggac aggctgcgca gggaggccag ctcgtcggcg tccgggaact
    17761 gggcggcgcg cttggccatg aggaaatcca tggcgccgcg gaagctgctg cgcaggtggg
    17821 ggtcgttcac cgcgccgtgg atgcgctggc gcagggcttc ggccgagacg atggtgatcg
    17881 ggcgggcgtc gccggcagga cggggcgctg tcatgctgag gctccttcgg cgtgggtgag
    17941 gatcaccacc agctccttcg ggccgtgggc gccgtaggcg agggtctgct ggatgtcggc
    18001 ggtcttggac gggccggaga tcagcagggc gttggtgggc aggcggtctg accactgctc
    18061 ggcgcgcagg gcatcgaaga aggtggcgtg gatgctgcgg gcatcgagca gcacgaagtg
    18121 caccggcggg accagggaca gggtgcgggg ttcggccgtc gagggccaca ggatcaggct
    18181 gccggtctcg gcgatggcgc cgcgggcggt ggtgaagccg gcgtcgatgc ggtggaagag
    18241 gtcggccttg agctcttcga agcggccctg ccagggacgt atctgcgtgg cgccaaaggg
    18301 tgtcgcgtcg atgcgttcac cggcgggcgt gttgcggccg tagagcagcg tgccgatgcc
    18361 cttggccgcg cacaggcggg cgagcagatc cggccagtcg gcgtcggcgc agacatgcac
    18421 ctcggcgtgg aaggcctcga tgccggagcg gaaccgggat gcccgctggg cgacggtttc
    18481 gtggcgcgca cgcggggcaa agtgggcgtc caggtcgggc aggggatcgg cggcggccgg
    18541 acgggtggcg cgcaggcggg cgaggatgct gttgcgggcg ctcatgcggg cttgcctccg
    18601 gtgcggcgca gcagaaagct ggccaggtgt tcggccggca gggatgcgcc gacgcggccg
    18661 gcatcccggt cttctttcgc cgcgcggcgg ctgatgttga gcatgcagcc gcagtcggcc
    18721 gtgaccatct gggcggcgcc ggtggcgcgc agggcgttga ccttgtcgcc gaccatggcg
    18781 gtcgagacat ccggatgctt cagggagaac gtgccgccga agccgcagca ttcggattcg
    18841 tgatcgtgca ccgcaagcgt cacctgcccc aggcgggcga ggagttcccg tccgaccagc
    18901 agggtgccca tctcccgacg cgcggagcag gaggtgtgca gggtgacggt ggtgggcgcg
    18961 ccggtgtcgg ggcggcggaa atcgagcacg tgcaccagga attcagccag ctcgaaggtg
    19021 cgctcggcca gcgccgcggc gcgggcggcg agggccgggt catcgccgac cacgccacgc
    19081 cagtgccagc gcatcatgcc ggcgcaggag ccggacggca cgacgatggg gtagtcctcg
    19141 gggaacaggt ccagttggga ggccaccacc tgacgggcct cgtccgggaa cccgctggtg
    19201 taggcgggtt ggccgcagca ggtctggttc tccggaaaga tcacgcggag gccttcccgt
    19261 tccaacaggg tgacggcgtc caggccggct tccggcacga agaggtcaac caggcaggtc
    19321 gcatagaggt aaaccgcggc cggtctggac gggtagcggc ggggcgtcgg cccgccgccg
    19381 ggaagggtgt cgctcatggt ctcctccttg tcctgctgcg aagccggcat tgtgggccgt
    19441 tcccgcggcg gtggggctca aggccccggg gtgtattcac tggtaaactg gtaagaccag
    19501 ttattcggtg gagtggaaat ctaaacggcg cgctcccgga agtcaatccc cgaaagcgtg
    19561 caaacccctg atttttgagt gatataaggg taaacaccga gacccgcggc ggggccctgc
    19621 cttgaggaac gggggatggc tgtgtagtat tctggtcaga ccaatttttt gagcatgaca
    19681 tgagcagcag caagatcgcg gtccagcgcc tttccgacac catcgcccac gaactcgagc
    19741 ggcgcattct ggaaggctcg ctcaagcctg gcgaccgtct gcaggcggag cgggagctgg
    19801 cggccgagct gggcgtgtcg cgaccgtccc tgcgggaggc gatccagaaa ctcgtgtcga
    19861 aagggctgct gcacagccgt cagggcggcg gtaccttcgt gacggaccgc cttgaagccg
    19921 gcttcaccga tccctggagc gagatgctgc gccagcatcc ggacatccag tccgacctgc
    19981 tggaattccg ccgcatgctc gagacggagt ccgccggcct ggccgcgcgg cgcgccaccg
    20041 acggggatct gcaacggatc ggcgacgcgt atgcgcgggt cgaaggcctg ttcgcccaag
    20101 ggccgtcggc ggaggtcttc gccgagcagg tgcgcgccga tctggccttc catcaggcga
    20161 ttgccgaggc ctcccacaac gtgatgatcg gtcatctgac ggccagcctg ttccgtgtca
    20221 cgcacgacaa tatcgaccgc aacctgcgcc acctgcgccg gcttgaggac gattggcagg
    20281 agttgcgccg ccagcacgcg gcgatctggg aaggactgcg cacccgcaat gcgggcgccg
    20341 cccaggctgc cgtcgagcgg cacattgcct tcgtggagga gagcatggcg gcgaccgcgc
    20401 gtcaggcgga gcgggagcgg cgggcgcaga cggtgcggat tcggtcgccg gagtaggact
    20461 gcggcctgca cgccggatca gatggcaggc accagatccg gcgcggcgcc cgcatgcatg
    20521 caccaggccg cgcggcgctt gccgcatgcc gggacctcca tgcccttgag ttttacagac
    20581 acccctccag cgttgcgcct gcgcgagaaa tgtttccgtg caacaggggt tctgatcgga
    20641 gcatgtggat ggcacaagct ccgcaccgtt tacgaggtca ggaggcgtca ggcgcccccg
    20701 gaatgtccgt ggggtgtcct cgagcctgcg ccggcgcctt gacgcctatg acgcgcgtgc
    20761 cggctgccag ggccgcccga atgcttcacg cctcgcattc gccggccatg cgtccgccgt
    20821 cggtgcggcc ggcatgaggg cgcggcgcta acgcccgcga tactgaccgc tccggccacg
    20881 actgccctca agtccgaccg ggcgcggcag acctgtcccg gcgcgctgct aagtcctttc
    20941 aggcgcgtgc cgcacgacga tctaccgggg gcgaggggaa ttgaccggtt cgcgtccggt
    21001 catcgcggcc cgggaagggc tggggctccg gaaaggatgc tgtgccgtcg gtctctcgcc
    21061 cacgcgcctg gcggcgggcg tcgtgtggcg gatccgggcg acgtcatgcg gtggcgagcg
    21121 atcacggaag ggggggacgg cgcgcacgcc catcgccggc catcgaccag cgggtgggtg
    21181 tcggaaaatc ttacactttt tggcaggtcg gcccgcctcc gggacgagat cggttgtgat
    21241 gcaaacctca ttatttcaat gggatggcgg gatgggggct gctccggtgc cggtcgggac
    21301 gtcgcgcggc caggccggat gccgcacttt tgcgctctgg gcgacctgaa ttgccgccaa
    21361 tctgtcatga aattttacgt tttttgccga acccgtctgg cgcagccggc aaatcccatt
    21421 ggaatgagtg ccttggtgaa gtggtatgaa taatgcattg gcggtcccgc ccataccatc
    21481 aaccagggac gtttttatgc aaaattcatc cagcgtgaac tttggcgcgc cgctgcgtgc
    21541 caagcttgag agctacgaag ccatcagccg tgtcgaggcc cggtccggca agtccgcccg
    21601 ggaaattgcc ggctacgccg cggccgccgg cgcggcgctc gccatggcgg gcaatgcgga
    21661 cgcgaccgtg atctacagcg gtgttcagaa tctcgtcgcc cagatcgatc cggcgcatca
    21721 gttcggcgtt tcttacgtga acggccatgc cggctacagc gtgaatttcc agaacttctc
    21781 cgtcaatggc gtgccgttca tggctggcgc ggtcattgcc gctgaactgg gaaccaacat
    21841 caacaacaat gcggggctgg ccgcgaagta catcggcgcg gcggtcgttc aggggtccgg
    21901 cgtccagttc gttgccggca gctcgtcccc gctgggcctt gccacgccgg ttgcgccggg
    21961 cgcggcgatc ggtgccggtt cgaaccagtt cggcggtgcg gccggtgcgg tgcgcatggg
    22021 cgtcggctac gtgaacggca agaccgtgca gtacggcaac ctcttgctgg gtgacaacct
    22081 cttcggcttt gcgctgccga atggcaattt tggctggatc cgcctgaaca tcgaagatct
    22141 gggcctgaat cagccgttct cggacatcct gcaaaacgcg ggcggcatca gccccctgtt
    22201 caacggtcag ggtttccccg accgcatcac ggtggtggat tgggcgtatg acgatcaggg
    22261 gcgcacgatc tttgccggta cgctgaacgt gcccgagccc ggtgcgctga gcctgctggc
    22321 ggccggggct gccggcatcg ccacgatgcg tcgtcgcaag ctgcactgat gtctgctgca
    22381 ccgggggccg atgtggcgcc ccggtgcctg tgactgccat gcatagacat gttgattcat
    22441 cgcgcttcgg tgcctatcgg atcgagaacg tccacctcct cacgagctcg gagcaacgcc
    22501 aggagatcgt gcagttctgg ctgcgcgaac gggccatccc gaccctgccg gaagccgtgc
    22561 ggcgggccca cgaggtctgc ctgctggtac gtcgcgacga cggcacaggc gccctcgcag
    22621 gcgtgtcgac cgtcggacgc atgcggaccc gcagcggact gatctactgg gttttccgca
    22681 tgttcctgcg caagccggat cgcgtaccgc gcctgagcct ggcggtggcc aacgccacct
    22741 gtgcgcacct gcgcctgcgg gcccggcgcg agggcgacgc ggccggcatg ctgatcgtca
    22801 ccgagaacgt caagctcatg cggcccggcg cccggatgct cttccagcgc cagggctgcc
    22861 gctatgtggg acgcaacgcc cgcggccagg acgtgtgggt cgtggatttc ccggcacacg
    22921 cgacggcagc ggcctgatcg atggtcgggg ccttcgcgtc cccgcgccgg ctggacgtcc
    22981 ttccgcaacc cctttccccg agaattctca acgcatgaac gcttcccgca aggtgctcct
    23041 ggtcggctgg gatgccgccg actggaaaat catcaatggc ctcgtcgaca gggggttgat
    23101 gccccacacc gcgtccctga ttgaacgggg tgtgatgggc aatctggcca cgctgcatcc
    23161 ggtgctgtcg ccgatgctgt ggaccaccat tgccaccggc aagcgcccct tcaagcacgg
    23221 cgttctgggc ttcaccgagc ccagccccga cggttcgggc gtgcagccgg tgagccagtg
    23281 gtcgcgcaag accaaggcgc tctggaacat cctcaaccag agcggcctga agagccacgt
    23341 gatcggctgg tggccgagtc atccggtgga gcccatcaac ggggtcatgg tgtccaacca
    23401 ttaccacacg gccctcggac cgccggacca gccctggccg ctggcgccgg gcatggtcca
    23461 tccccctcgg gcgcagcagg cgctggccga cgtgcggctc aatcccaatg agctgctgcc
    23521 tgagcaggtc tgcttcttcg tgccgcgggc ggcggagatc gaccaggaca aggaccgccg
    23581 cctggcgtcc gtgatgaaga ccctcgccga atgcaccagc gtccatgccg ccgcgacgtg
    23641 ggccatggcg aacgagccgt gggacttcat gggcgtgtat tacgacgcca tcgaccactt
    23701 ctcccatggc ttcatgcgct atcacccgcc gcggcgtccg tttgttcccg agcgggactt
    23761 cgagctgtac cgccatgtcg tcgaggcggc ctaccagttc cacgacctga tgctgggcac
    23821 cctgcttcag ctggcgccgc cggacaccac cgtgatcatc tgctcggatc acggcttcca
    23881 ttccgatcac cgccgtcccg cgcagattcc caacgagccg gccggtcccg cggtggagca
    23941 ccgcgacctg ggcatgttcc tgatcgccgg accaggcatc cgcagggatc acctgatcca
    24001 cggcgccagc gtgctggacg tgacgcccac cgtgctgacc ctcttcggcc tgccggttgg
    24061 cgaggacatg gatggcaagg tgctgctgga tgtcttcgac cggacccccg aggtggccca
    24121 cgtgcccagc tgggacgcgg tacccggtga cgatgcgcgc ctttccggcg agcagcagtt
    24181 cgaccccctg gccgcgaagg aggtgatgga tcagctggtg gccctcggtt acatcgaaaa
    24241 gctcgacgac aaccgcgaac aggccgtggc ccagacggtg cgtgaactgc gttacaacct
    24301 cgcgcggagc tacatggacg ccgggcgcat gctcgacgcg gcgcccctgc tccaggaact
    24361 ctatgccgcc gagccggacc agtatcgctt cggcgtgcat ctcgccctgt gttaccgggc
    24421 cctcaaccgg gtggccgaac tgcgcccgct ggtggagcgc atgacccgcg accgtcagga
    24481 agccgcgcag acggcccgtg cggccctgca ggcgctctgc gacacggtgc gccagcgtca
    24541 gcaggctgcg gcgacgacga ccgccgtccg cgcgcaggac gatccgccgt ccgccgccga
    24601 tgcgctggcg tccatcgacc tgagccagct caccgaggac gagcgcagcc aggtggacga
    24661 gctgcgattc aaggcatggt tctccacctc cgacctggac tatctgatgg gctgggtgct
    24721 gggcgccgag gggcgtcatg atgaagcgct ggcgcacctg ctcaaggcag agcgggcgga
    24781 tccgggccgc ccgggcctgc tggtgcagat cggcgaagcc catcttgcgg tccaccgctg
    24841 ggacgaggcg gaagccgcct tccgccgggc cctggcggcc gatgcgctca gtccctatgc
    24901 ccatctcggt gtggcgcgcg ccttgctgcc gcgccgcgcg cgcgccgagg aggccgtgga
    24961 ggccgccctc gattccatcc ggctgctgta ccagaacccc ctggcccatt acgtcctggg
    25021 cctggggttg ctgcgcctgc ggcagttctt catggcggcc gatgccatgc gtactgcgct
    25081 cgccctcaat cccaacttcg tgcgtgccca ccgggtgctg gcccgcttct accgtctggg
    25141 aaccggcgag catgacaagg cgcgggccca tctcgaggcc atccagcaca tccgtctcgg
    25201 caagcgtgca gcgtccatga ccggtcagcc cggtctcggc gaggccgaat cccttgccca
    25261 tgaggtggac gacgaggccg gccggcccgg actgcatgcc gacgccgtgt tcgccgtgcg
    25321 caagacgccg gacgacccgg ccgaattcgt cactgtggtc gccggtctgc cccgttcggg
    25381 cacatcgatg atgatgcaga tgctcgccgc gggaggactg ccgatcctca cggacaacaa
    25441 acgtgaagcc gacctcgaca atccgcgtgg ctatttcgag ctggaagccg ccacccgcct
    25501 gcggaccgac cggacatggt tccgcgaagg acggggccag gcggtcaaga tcgtggccca
    25561 gttgctgccc tacctgcccg gcgacgtgcc ctgtcgtgtg atcttcatgg agcgcgatct
    25621 ggacgaggtg ctcaagtccc agaccgtcat gctcgacaac ctgggccggg aaggggcgcg
    25681 actcagcgat gcgcaggtgc aggccagcta ccgccagcag ctgcgcaggg tgaagctgtg
    25741 gctggcgcgt cagtcccagg tggacgtgct gttcgtgcgc caccgggacg ccctcgccga
    25801 cccggcggcc gttgcggaac aggtgaatgc cttcctcggc ggacgtctgg actgcttggc
    25861 catggcgacc tgcgtggatg caagcctgta ccgccagcgg gccacggcat gacgacggac
    25921 acgcagcggc ccacggtgat ctggcatccg ggcgatgtga cacccgcgat gcgcgaacgc
    25981 ctgaacgggc atcgcgggcg ggtgctgtgg ttcaccggcc tctcaggggc cggcaagtcc
    26041 acgctggccc acgcggtgga ggcctgcctg cacgagcagg gccggcgggc ctttgttctg
    26101 gatggcgacc acctgcggca cgggctgtgt gccggactgg gcttctcgtc ggcggaccgc
    26161 agcgagaacc tgcgccgggc ggccgaggtg gcgcggctgt ttgcggatgc ggggaccgtc
    26221 accctggcgg ccttcatctc gcccttgcgc gaggaccggg agcgcgtgcg ggccatcgtt
    26281 ggcgcggatc gctttgtgga agtgtacgtg gcctgcccgc tggccgagtg cgagtcgcgt
    26341 gacgtcaagg gcttgtacaa gcgcgcccgc gccgcggaaa tccctcactt cacgggcatc
    26401 tcggccccct acgaagcccc cgagcatcca gacctcgtgc tcgataccag ccgaacaccc
    26461 atcgaggcgt gcgtggcaca ggtgctggca tgcgtcgacc ggatcgtgtc cctcgaggcg
    26521 gacaccttca ccgacatcgg ggagtcgatg catccggccc cgaccgaccg ggcaccgctg
    26581 acgcgctcgc agcgtgtgga ggtgtaagtg cgcgcggcgg gtcctggact gaagccatgc
    26641 gcaccacgcc cgacatcaac gccacgcctg ccgccggaca ctggcaggac ttcagcgccc
    26701 cggtgacgct taccgccgaa caggccgccg cgctgtaccg gtcggtcaac gacgtggcaa
    26761 cgctgctgtc cggcgccatc caactggccg agaacgccga cagcaccctt gaggcgcttc
    26821 acggctgaga tcgtgcggcc gcaaaggagg gggcgatgca gaccgaacgc tatggaaccc
    26881 gcgatcaggc ttattcggcc tggcatcgct gccagagcac ccgccgctat gtcgggatcg
    26941 agcaagcggc ccggctgtcc atgatcgacc tggacggcgc cctgttcgtc gagtacgaca
    27001 acggcgaccg ggagccgctt gccctgatag agacggcgcg cgacgtgggg cagccggtca
    27061 agccgtgcac ggtaacggcc cgtctggccc agcgttcggg gctcccggcc tacgtggtgc
    27121 tgtaccgaac ctccgacctt ccgaaccctg cggacgagcg gcagcccgac attcagagct
    27181 tccgcgtccg caggatctgg ccccatccag agccggcatg gcgcgtgctg tctccccgga
    27241 gttgggcgga ggcgctgcta cagatccggt agtggtcgtg ctcgcggctg gatagcgtgg
    27301 cggcgaacga tccgcggtgg tgatctgcga gttcaagcgg gcgatggtcg cgcgaagccg
    27361 gtcgatcaag agcgcctgct ggtagctgtt gcgctctggg ctctcgccat ccggccatgt
    27421 ccgggccgta gggcgacgtg taacccacgt cgtccagcca gccgagggct tccagttcga
    27481 gttggtcttc ggtcatcgtc cgtccgtgat ttttttcgcg ctttttcggc ctgtttaaac
    27541 tacaattgag tcctaagtgg attgggggtt cccggtcggc gtcgtagcct cggtgggttc
    27601 accggggctt ttcgctttct tgggtggata aattttcagt ccaacattct cgtaaccgca
    27661 gcccaccttg ttccttcccc catcgcagaa gaacttcttc ttcagcatct cgaatgcctt
    27721 gttgccctgc tccgggcgaa gatagttaat ccccacggga cgggccacca gatctgccaa
    27781 ctgcaatccc gcgaggttgg tcttcttgtc ggcgaaaacg atatcaaacg gcaacgcccg
    27841 gttgctgggg ttatcaccgt cgcagatgcg cctgaactcc agttccagct cagcatcttc
    27901 tttcttgccg cggcactcca ctaagacgtg cgtcttgaag tcgtcctgtt tcttctcggc
    27961 caggaactcg cgcagggcct caagacaaac tccaagtgca atgtgatacg ggtttgagtt
    28021 tgccccttcg ttgcggctca agcgaacctt gtcgacgaca caggagatca ggatgaagtt
    28081 gctggcgccc atcaccgacg acaggcggtc catgaactca tcgcgtgtgg ggaggtggct
    28141 caggaaggcg aagtcccctt tctgcttgcg aatctcgttc tcatgcaaga ccacgctatc
    28201 gtgaccaaag tagttgaact tcagcttctg gacggcaggg acgatcttct ctgcgtagtg
    28261 tcgcttgtgg aaaacgcaca gtgccagtac gaacacggga tagccttggt caatgctggc
    28321 cagagagtgg tcgccgcttt catcgacata gacgatgtaa tcactgaacc tccgaccagc
    28381 aggctcttgg gcaggctgct cgaccgggat tgcctcgacg gattcgaaca gagagtactg
    28441 ggttcctgga atggtcatga agttaagtcc agttcgtacg catcacgcga tttcccccaa
    28501 gacgaactct ggctcttctg gggcgctgct ggccggggat caggcggggc agggtatcgc
    28561 ggagggtaga gatggtttgg gcttggggtg gttggcggca atccgctgct gaacattcgt
    28621 aacaactgag ccgaaagcct cggcgacgtc aggactgggg atggcgaccg gaaggcggcc
    28681 gaacacgtcg tcctgtagac gccgctccca gatcccggca atccccattg gctttccgtc
    28741 tgcccgttca atgcgccagc gtaccgcatt gccggattcg tagcagggct caaagatcgc
    28801 agcggcgggg atgatgcaaa gttgcccgcg cttccatgcg ctgcagaatg agggcttctc
    28861 gcccacggtt tcagagcgcg cgttgtaggt agaacggacg atccgtgcat ccttcgccca
    28921 catgggcacc agtccgaacg atgccggcac ccagaggcac ggcgcaacgg tggtgacgat
    28981 gggcacaatg gagccggggt aagcctcccc atagccgaag tccggcggtg gcaggggaaa
    29041 ggctgtcagt tcttcgcggg acgaggggag gtagttggcg cacatgggca cagcgtagcg
    29101 cgcctttgac ggctgccgat aggccgtagc gctttccggg ccgccatcgc cagcataggc
    29161 ggcgcgtgtt gggttttgca ccaaacacac ggagccgggt gagagtggac ggtcacttcg
    29221 tcacgcatcg atgttgaacg ctgcgggagc aataggggag caacgagccc ggaaacgaca
    29281 aaggccaacc ccgaaggatt ggcctaagtc tttgattctg ttggtggtga tgggcagaat
    29341 cgaactgccg acctacgggt tatgaatccg tcgctctaac cgtctgagct acatcaccac
    29401 actgagcccg ctataatagt ggaccgaatc caccctggtc aagaaaaagg tcatgaagaa
    29461 gttgtatatc cgtacgttcg gctgccagat gaacgagtac gactccgaca agatggcgga
    29521 cgtgctcggc gctggcgaga cgctggtgaa gacggacaat ccggaggagg ctgacgtgat
    29581 cctgttcaac acctgctccg tgcgcgaaaa ggcgcaggag aaggtgttcc acgatctggg
    29641 gcgggtgaag cacctgaaac agaagaaccc tgggctgatc atcggcgtgg gcggttgcgt
    29701 ggcgagccag gaaggggagg ccatcgtgca gcgggcgccc tatgtggatc tggtcttcgg
    29761 accccagacc ctgcaccggc tgcccaagct gatcgccgag cgcaaggcca ccgggcgccc
    29821 ccaggtggac atctcctttc cggagatcga aaagttcgac gcgctgccgc cggcgcgcac
    29881 ggaaggggcg agcgccttcg tgtcgatcat ggagggctgc tccaagtact gcaccttctg
    29941 catcgtgccc tatacccgtg gcgacgaggt ctatcgcccc ctggccgaca tcctggccga
    30001 ggtggcgggg cttactgccc agggcgtcaa ggagatcacc ctgctggggc agaacgtcaa
    30061 tgcctggcgt gcgccgctag acggggccgc aggcgagatg gcggattttg ccttcctgct
    30121 cgaatgcgtg cacgagatcc ccggcgtgga acgcctgcgc tacacgacct ctcatccccg
    30181 cgagatgacc gcccgcgtgg tggacgcgta tgcccgcctg cccaagctcg tgtcccatct
    30241 gcacctgccc gtgcaggccg gctccgaccg ggtgctggcg gccatgaagc gcggctacac
    30301 ggtgctcgaa tacaaatccc tggtgcgcag gctgcgtgcg gcgcggccgg acatctccct
    30361 gtcgtccgac ttcatcgtgg gcttcccggg cgagaccgag gcggacttcg agcagaccat
    30421 gaagctcatc gacgaactgg gcttcgacac ctccttcagc ttcgtctaca gcccgcgacc
    30481 cggcacgccg gcggcggaca tggccgacga cacgccccag gacctcaagc tggcccgcct
    30541 agcgcgtctg cagaaagccg tcgaggccca cgccgcgcgg atcagcgaag ccatggtcgg
    30601 ctcggtgcag cgcatcctgg tggagggccc gtccaagaag aacccggcgg agctggccgg
    30661 gcgcaccgac aacaaccggg tcgtcaactt cgccggcaat tcgcgcatga tcggccactt
    30721 cgtggatgtc accatcgtct ccgcgctgcc ccattccctg cgtggcgaac tggtgctcaa
    30781 ggactagaat ggcgcctttc gggccggtgc ccgcgtcctc tccaaacctc cccgcgtgaa
    30841 gaacaaagct ctggaagtgg ccttcgagcc cgccgacaac acccggctgg ccaacctgtg
    30901 cggcgtgctg gacgaaaacc tgcgccagat cgccaccgcc tacgacgtgg agatcgcccg
    30961 tcgcggcgag cgcttcagcg tccgcggcca cctcgtccag gcgcggctgg cggcgcaggc
    31021 cctgcagcat ttctatgatc tggccggcga gcacctgagc atcgacaccg tgcaactggg
    31081 cctcatcgaa ctcgccaacc gcagccaggc cagtcactcg gcgccggctt tgctcacccg
    31141 caagaccgaa ctccacggcc gcacgccgcg ccaggtggac tacatccgcc agatccagga
    31201 gcacgacatc accttcggcg tcggcccggc cggcaccggc aagacctacc tggccgtggc
    31261 cagcgcggtg gatgccttcg agcgggagca ggtggagcgc atcatcctga cccgcccggc
    31321 ggtggaggcg ggcgagcggc tcgggttttt gcccggcgat ctggcccaga aggtggaccc
    31381 ctacctgcgc ccgctctacg acgccctcta cgacctgatg ggcttcgacc gggtcgccaa
    31441 gctcttcgag cgcggctgca tcgagatcgc gccgctggcc ttcatgcgcg gccgcaccct
    31501 gtcccgcgcc ttcatcatcc tcgacgaggc ccagaacacc tcgccggagc agatgaagat
    31561 gttcctcacc cgaatcggca tcggctcgcg ggccgtcatc actggcgacc tgacccaggt
    31621 ggacctgccc cgcggccagc gcagcggcct tggcgaagcc ctccaggtgc tcgccgacgt
    31681 gcgcggggtc gccttcaccc gcttcctcaa ggaagacgtg gtgcgccatc ccctggtcgc
    31741 gcgcatcgtg gccgcctacg aatcccattc ggccaccccg gccccgacca catgaccgcc
    31801 tccgcgctgc cccgccgcct cgatctgtcc gtgcaatatg cctgtcaccg ggatggcgta
    31861 cccttgcggg ccgacttccg gcgctggctg cgggccgccg aatccggcgc cgcgcgcatc
    31921 accgtgcgca tcgtggatga agccgagggg cgcgaactca accgggatta tcgcggcaag
    31981 gattacgcca ccaacgtcct caccttcgcc tacgccgagg gcgaggacat gccgatgccg
    32041 gaaggcttgc cgctgatggg cgatctggtg ctgtgccgca gcgtcgtgga gcgcgaggcg
    32101 gccgagcagg gcaagcccct ggcggcccat tacgcccatc tggtggtgca tggtatgctg
    32161 cacctgcaag gtttcgacca tgaggccgac gccgacgccg agctcatgga agcccgcgag
    32221 cgggccatcc tggccgaact ggggtacgac gacccctacg cgctggagca ctgacgggac
    32281 ggggtgagca atgcgccatg ggcgatgtgt ccatcgccca tggcccattt ctcatccacc
    32341 ccttttatgg aaccctccag tagtaaaccg tcattgcttg aacgactttc ggccctgctc
    32401 acgcgggagc cggaggatcg cgaagaactg ttgcacctgc tccatgccgc gcatgagcgc
    32461 aacctgttcg acgcggacgc cctggccatc atcgagggcg ccctgcaggt gtcggacatg
    32521 cgcgtcgccg acgtgatgat cccgcgggcc cagatggacg tcgtccgcgt ggacgacagc
    32581 atcgaggaca tcacccgctt cgccatcacc gccgcccact cccgctttcc cgtcatcggc
    32641 gaaggcaagg acgacgtgat cggcatcctc ctcgccaaag acctgctgcg ggtcttcgcc
    32701 ggcgaagcct tcgatctgcg cgacatgctc cggccggcgg tcttcgtgcc ggaatccaaa
    32761 cgcctcaacg tgctcctgcg ggaatttcgc gtcagccgca accacatggc catcgtcgtg
    32821 gatgagtacg gcggtgtcgc cggcctggtg accatcgagg acgtactcga acagatcgtc
    32881 ggcgacatcg aggacgaata cgatttcgac gaggccgccg acaacatccg cctcgaccag
    32941 gccgggcgtt accgcgtcaa ggccggcacc gagatcgacg acttcaatgc tgccttcggc
    33001 accaccttca gcgacgacga gtacgacacc gtcggcggcc tgctgatccg caagcttggc
    33061 cgcctgccca agcgcaatga ggtgatcgtc ctcgacaacc tgcgctttca gattctgcgg
    33121 gctgacagcc ggcgcgtgta ttccctcctc gtggagcgca cgcctcaggc cggcgtgatc
    33181 ggcggctgat gtcccgcctg cgtccggtcc tcgcctgggc ggcgggggcg gcgtcggtgc
    33241 tcgcctacgc cccattcgag tattttccgc tggcggccgt ggccctggcc gtgctgtacg
    33301 cgctgctggg ccgcgcggcc gatgcggcca tcacgcccgg cgcccgccgg cgggcgggat
    33361 tcctcatcgg ctgggcctgg ggcttcggcg ccctgggggc cggcgtgtcg tggctgtacg
    33421 tggccctgca ccgattcggc ggcatgccga tgcccctggc ggggagcgcc atcgccctgt
    33481 tctgtgccgt gatggcgctc ttcgccggtg gggcgggagc gctcttcgcc gatctgcggc
    33541 gggggcggcc atgggcggac gcggccctct tcggcgcgct gtggctgctc accgaatgga
    33601 cccgcggctg gctgttcacc ggttttccgt ggctggtcag cggctacagc cagacgccgc
    33661 cgagcccgct ggcgggcttt gcgccggtgt tcggcgtgtt cggcgtgggc gggctgctgg
    33721 cgtggatcgc cgcgctgctg gccgaagcag tgacgcggcg gagaatcgcc tgcgcgggtc
    33781 tggtcctgct tgtcggcgcg gggggcgccg ccctgcgtcc ggtggcatgg accgtacccg
    33841 tcggcgcgcc gctgcaggtg gcgctgctcc agcccaacat ggagcaggat ctgaagtggc
    33901 ggcccgacat gctgccgcgc tggctggaca tccacctgga actgctgcgg aacaacccgg
    33961 cgaccctcgt ggtgttgccg gaaaccaccc tgccgctgct cctggagcat ctgcccgacg
    34021 gttatctgga cgccctcacc cgcagtgccg cggtgggcgg cgacgcgatc ctcggtgcct
    34081 tcgtgcggga tggggacggg cgcatcttca acgccgccgt cagtgtgggg cgctcgccga
    34141 cccagcatta cgccaagcat cacctggtgc ccttcggcga gtactcgccg cccctgttcc
    34201 gctggttcta cgatctggtg cacatcccca tggccgacca gacccgtggc gccgaggtgc
    34261 agccgcccat gagcctggcg gggcaaaaga tcgcggtcaa catctgttac gaggacgtct
    34321 tcggcgagga gctgatccgc gccctgcccg aggcgaccct gatgctcaac ctgtccaatc
    34381 tggcctggta cggggactcc tttgcccagc cccagcacct gcagatcgcc cgcctgcgtg
    34441 cgctcgagag cgggcggcca atgctgcgcg ccaccaacac cggcatgacc gggctggtcc
    34501 agcccgacgg cacggtgagc gccgcgctgc ccgccttcac ccgcggtgcc ctgcaggtgg
    34561 cggtgcgcgg ctacaccggc tcgacgcctt acgggcgctg gggcaacggc ccggcggtgg
    34621 gcctcgccgc cctcgtggtg ctggtggtgc tggccgggcg ggtgcgcggt cggaccccga
    34681 tctaagtcaa atctccgccg acagtggcgg cgtatcatcc ctatcgcctt tctcccctcc
    34741 aggtgctcca ccatgatgcg tccttatccc acgaacagcc ccgaagccct ggcccgcctg
    34801 gtggccctgg caatcctcgc cgatggccgt ctgcacaacc aggaggtcac ctggatccgc
    34861 gagcacgata ctgcgtccct catcggcgtc gagcaggaca ccctcgtgca ggtgctcctc
    34921 gactgttgtc gcgatctgct cagcgaaggc gggcgtggtc gcgtggatct gctcagcgaa
    34981 gcccgcctgg ctccgctggc cgaggagatt gccgatcccg ccctgcgcaa ggtggcgatc
    35041 tccgccgtgc tggtggtggc caaatcggat ggccgcctga gcagcgaaga acagaccctg
    35101 ctgcgtttcc tgttgtcccg ctgggacatc gaactgcagg agctggtctg atccgaaggg
    35161 ccgcgcagcc tgctgcgcgg ccaccgggcg gcgcccgcac ggcgctgtcc ggcacctgtc
    35221 cgcataacaa caagtccatt tcaaggagat cgcccgtgag cgaccagatc aagtatctgc
    35281 tcgacgaatc ccgcctgccc aaggcctggt acaacatcgc cgccgacctg ccggtaccgc
    35341 tgccgccgcc ccttcatccg ggcaccctgc agcccctcgg gccggcggat ctggcccccc
    35401 tgttccccaa cgccatcatc gcccaggagg tctccaccga gcgctacgtc gacatccccg
    35461 agccggtgcg cgacgtcctg cgtcaatggc gtcccagccc cctgttccgc gcccgccggc
    35521 tggagaagct gctcgatacg ccggccaaga tctattacaa gtacgaaggc gtgtcgcccg
    35581 ccggcagcca caagcccaac tccgccgtgc cccaggcctg gtacaacgcc caggagggcg
    35641 tgaagcgcct gtctaccgag accggtgccg gtcagtgggg gtcgtccctc gcctacgcgg
    35701 gcgggctctt cggcctggac gtgacggtct tccaggtgcg cgtgtcctac gaccagaagc
    35761 cctaccgccg cgccctgatg gaaacctggg gcgcccgctg catcgcctcg ccctcgctgg
    35821 agaccgagag cggccgcgcc gtcctggcga agacgccgga ccatctgggt tccctgggca
    35881 tcgccatctc cgaggcggtg gaagtggcgg cgaagcgcga cgacaccaag tacgcgctcg
    35941 gctccgtgct caaccatgtg ctgctgcacc agaccatcat cggtcaggag gccatcgccc
    36001 agctcgagat ggcgggtgac gatccggatg tggtgatcgg ctgcactggc ggcggttcca
    36061 atttcgccgg catcgccttc cccttcatcg gcctccagct gcggggcggg gcgaacgccc
    36121 gccggcgccg catcctggcg gtggagccct cggcctgtcc gtccctcagc cgcggccagt
    36181 acgcctacga cttcggcgat accgcccacc tcacgccgct ggtcaagatg cacaccctgg
    36241 gcagcgaatt cacgcccccc ggctttcacg ccggcggcct gcgttaccac ggcatggcgc
    36301 cgctggtgag ccatgtgaag gagctgggtt tcgttgaggc gcgcaactat caccagaacg
    36361 cctgcttcga ggccggtgtg atgttcgccc gcagcgaagg catcgtcccg gcgccggagg
    36421 ccaatcacgc gatccgcgcc gccatcgacg aggcgctgcg ctgcaaggcg gaagggcgca
    36481 gcgagacgat cctgttcagc ctgtccggcc acggccactt tgacatggcg tcgtacacca
    36541 attacctggc cggcaagctg gtggatcagg attacgacga acaggaactg gccatggccc
    36601 tctccggcct gccccgcgtg ccggtgtgat ggcccccgag cgggccgccg cctggcatgc
    36661 ccgctcgccg caggacgtcc tcgacgccct gcgtagttcg ccccatggcc tgacgacggc
    36721 cgacgccgcc gagcgcctgc ggcggcacgg ccccaaccgc ctccccggcc ggcgagggcc
    36781 cgggccctgg cagcgcctcg cccagcagtt ccatcagccc ctcatctaca tcctcctcgc
    36841 cagcggcgca atcgccctcc tcctgggcga atgggctgac gccggcgtga tcttcggcgt
    36901 ggtgctggtc aatgccctga tcggcttcgt ccaggagggc aaagccgctg acgcgctggc
    36961 agccctggcc cgcagcgtgg cgagcacggt cacggtgctg cgcgacggcc ggatggtcgc
    37021 gtgcgaggcg gtggagctgg tgccgggcga cgtggtccgg ctcggtgccg gtgacaaggt
    37081 gccggccgac ctgcgcctgt tcgacgcccg cagcctgcag atcgccgagg cggccctcac
    37141 cggcgagtcc gtcgccgtcg ccaaggacac ggcgacggtg ccggcggacg gggtgctggc
    37201 cgagcgggcc tgcatggcct atgccggaac gctggtgacc gcgggccagg ggtgcggggt
    37261 ggtcgtggcc accggcgcgg acacggaagt cggccgcatt gctcacttgc tggacgtcac
    37321 cgatgccctg gcgacccccc tgacccgtgc catggccgga ttttcccggg tgctgctgtg
    37381 gctgatcctc ggactggcgg tcttcggctt cgtggtcggc tggatgcgcg gtgaagccct
    37441 tgcggacatg ctcatggcgg cggtggcgct ggcggtgggg gcgattcctg aagggctgcc
    37501 cgcggcggtg accgtcaccc tggcgattgg cgtggcgcgc atggcccgcc gcaaggccat
    37561 cgtccggcgc ctgccggcgg tggagactct gggcagcacc acggtgatct gctccgacaa
    37621 gaccggcacc ctcaccgaga accggatgac ggtgaaggtc ctgtggcagg gcgggcggtc
    37681 cttcgaatgg gcagaaaaca cgccggaacc ctccgccgag cctggacgcg ccgggctgcc
    37741 ggccggcctg aacgaactgc tcgtggcggg cgtgttgtgc aatgacgcgc gcctgcaggc
    37801 ggatgccgcc ggcaggccgg cgagcggcga cccgacggag gtggccttgc tcgtggccgc
    37861 tgacgtgggc ggactcgacg gcgctgcgct acgcggcgcc tgtccgcggc ttggcgaaat
    37921 ccccttcgat gccgcgcggc cttacatggc cgtgaaggtc tgtgtggcgg accacccggt
    37981 ggtgttcgtc aagggcgggc tggaggcgct gctgccgcga tgcgcggcgc agatcggcgt
    38041 ggatggatgc ccggaggcgc tcgatcccgc cgccgtggag gtggccgcca cggcgctggc
    38101 cgctgacgga ttgcgggtgc tcgcctttgc gcggggcggg gacggcacgc tgggtgcgga
    38161 gctgccggct caggggctgg tcttcctggg cttgcaggcc atgcaggatc ccccgcggcc
    38221 ggcggcgatc cgcgccgtgg cggcctgccg tgcggccggt gtcacggtga agatgatcac
    38281 cggtgaccat gccgccaccg ccctcgccgt cgcgcggcag ctcggcatcg ccgccgacgg
    38341 tgacgccgtg ctcaccggcc gcgatctggc gggtctcgac gccctcgccc tgcaggccgc
    38401 ggccatgcgg gtgaacgtgt tcgcccgtgt ggagccggcc cagaagctgc ggctggtgca
    38461 ggcgctgcag gcggggggcg aggtggtcgc aatgaccggc gacggggtga acgatgcgcc
    38521 ggccctcaag gcggccgaca tcggcgtggc gatgggcgcc ggtggcaccg aggtggccaa
    38581 ggaggcggca gcgatggtgc tcacggacga caacttcgcc accctggagg ccgcggtgga
    38641 ggcaggacgc ggggtcttcg acaatctggt gaaattcatc ctgtggaccc tgcccaccaa
    38701 tttcgccgaa gggctggtga tcctcttcgc ggtgctggcc ggcaccaccc tgcccatcac
    38761 gcctttgcag atcctgtgga tcaacatgac caccgccgtg ctgctgggca tgaccctggc
    38821 cttcgagccc atcgaaccgg atgtgatggg ccgcccgcca cggccgcccg gtgcgccggt
    38881 gctggacgcg gtgcagatgc gccgcgtcgt gctggtgggc gcgctgctgg tggcggcggc
    38941 cttcggcctg ttcctgcgct ggcgcgggtt cggggcgagc ctcgaggagg cgcgcacgat
    39001 cgccgtgaac gtctttgttg ccggcgaggt ggcctatctt ttcaactgcc gcagcctgcg
    39061 cggcggcttc tggcgcggcg gcgtgctcgc caacccgtgg ttctggctcg ggatcggtgc
    39121 gatggtcgcc ttgcaggcgg cctttacctg gtggccggcg atgaaccggc tcttcgcatc
    39181 ggcgccaccg ccgccggccg cgtggctcga gatcggggcg gcggcctggg ccctggcgct
    39241 ggcggtcggc ttggaaaagc gcctgacggg cggatcggca tcccgcccag gcgcggcgcg
    39301 gatcgcccgc tagacgcgga agtggctgac ctggtcgcgc aggctgccgg cgatgcgttc
    39361 cagtccgacg gccgcctcgg cggcgccgcg gatggtcttg gtggtgttct ccaccatgtt
    39421 ggagatgtcc tcgacgcgct gggcgatggc cgtgctggca gcgctctgtt cgcgggtggc
    39481 gtcagcgatt tcgtggaccc gttcgagggt ccgggtggcg ccgttctcga tggccgccag
    39541 ggattcggcg gccgaggcgg cgaggctgac gccctcgtcc acctcgggca gggcggcgtt
    39601 catcgcctcc acggcgctgc ccgtgtcgct ctggatgccg acgatcatct gctcgatctc
    39661 ggtggtggcg gaggaggtgc gttcagccag cttgcgtact tcgtcggcca ccacggcgaa
    39721 gccgcggccc tgctcgccgg cgcgggccgc ctcgatggcc gcgttgaggg cgagcagatt
    39781 ggtctggccg gcgatctcct tgatcacgtt ggcgatggac gacacctggg agatgcggcc
    39841 ttccagggca cggatgcgcg tggaggcacc caagacggtc tctgacatct tgcggatcgc
    39901 gccagaggcc tgctggacac gctgggtacc ctcggcggcg aggcgcatgg cttcctgaga
    39961 gttgtcctcg gtgtcacggg cgctgctcga gatgtggctg gaactgacag tgagctcctc
    40021 catggccgcg gccatggccg cggtggcgtc ggactggcgg atggcggcgt cggccacttc
    40081 accggacacc tggctgatgt ggtcggcgtt gctgacgagc tggacggcgc tgcggttgat
    40141 ctcgcccatc atggtgcgca gcgacgcgat catggtggac agggcgtgga gcatggagcc
    40201 ggggcggctg tcctccaggc gcagggagag gtcgccgtcc gccacctgct gcatgatggc
    40261 cgtcgcctcg cggggttcgc cgccgagttg ccggatgatg ctgatgccga cgccccagcc
    40321 ggccacgccc agaacgatca gcagcaccgc ggagatgccg cccagggtcc aggcgatggc
    40381 gcggaattcc acgtccacgt catccacgta gatgcccgtg cccaacaccc attgccaggg
    40441 accgaactgg gcggcaaagc tcaccttggg ctgcggatcg gttccgccgg gcttggggaa
    40501 gaggtaatcc acgaagcctc ctccggccag ggcggccttg accagttccg ggatgatggc
    40561 cttgccggta ctgtccttgg tgccactggc gtcggtgcct tccttctcgg ggcgaggagg
    40621 catcaggatg taatggctgg tggtgctcac cacgaagatg tagttggtgc cctcgtagcg
    40681 cagggtacgc agggtttcga tggccgcctt gcgggcgtcg gcatcggaga gcgcgccgga
    40741 ctgggcgagg gcgtggaaat gctggagcac cccgaggccc agctggacga cgtagcgggt
    40801 ctgctgcttg cggtcgtcca tcatgctggc gtgcagccgg aacaggctga cggtgcacag
    40861 cgcgacgaga ccgatgaggg tcagcgcgac gagaatccgc atccgcgtcg caacacgcag
    40921 ttcggacagg ttcatgacga gggctctcca ggacggacgt acacgggaat caacgggcgc
    40981 ggcggtggat atgtatcaat ttgtatattg gctgccatta cggcagaaaa tacccctgcc
    41041 ttgagacggg tctatgtgat ctgttccacg gagcgcctcc cggcgggaca ggagcctgcc
    41101 gagtccggcg aaacccagta aaatccgcgt tttgcccttt ctgcgcggct caccgccgcc
    41161 gagcgtccat gtcagtccat aaaccgactt tccagcagat catcctgcgg ctgtccaact
    41221 tctgggcgga acgcggctgt accctgctcc agccctacga catcgaggtg ggggccggca
    41281 ccttccacac cgccaccttc ctgcgcgcca tcggcccgga acactggaac gccgcctacg
    41341 tgcagccctc ccggcggccc aaggacggcc gctacggcga gaaccccaac cgtctgcagc
    41401 actattacca gtaccaggtg gtcctcaagc cctcgccggc caacatccag gagctgtata
    41461 tcgacagcct gcgggcgctg ggcatcgatc ccaacgccca cgacatccgc ttcgtcgagg
    41521 acgactggga gtcgcccacc ctgggcgcct gggggctggg ctgggaggtg tggctggacg
    41581 gcatggaggt gacccagttc acctacttcc aggaggtcgg ctccctgagc tgcaagccgg
    41641 tgctgggcga gatcacctat ggtctggagc gcctggccat gtacctgcag ggcgtggaaa
    41701 acgtctacga cctggtctgg tcgatcggcc cggacggcac gccggtcacc tacggcaacg
    41761 tctaccacca gaacgaggtg gagcagtcca cctacaattt cgagcactcc aacgtggaat
    41821 ggctgttccg cctgttcggc gaatacgaag ccgaggccaa gcgcctgatg gccgtgcccc
    41881 tggtgctgcc ggccttcgag atggtcatga agtgctccca caccttcaac ctgctggacg
    41941 cccgcggcgc catctcggtc accgagcgcg ccgcctacat cggccgcgtc cgcgccctgg
    42001 cccgggaagt ggcccaggcc tatttcaatt cccgtgaagc cctcggcttc ccgatgctga
    42061 agaaggaggc cgcgtgatga ccggcgcgac cctgcttgtc gaactgctca ccgaagagct
    42121 tccgcccaag gccttgccgc gcctcggtca gacctttgcc aagggtgtcg tcgaaggcct
    42181 gcgcgcccgc ggcctggccg cggcggaggg cgccttccgc tggttcgcca cgccacgccg
    42241 gctggccgtc agcgtgagcg gtgtggtggc cgaggccgcc gcccgcgacg tgaccgaaaa
    42301 gatcatgccg gtgtccgtgg ccctcgacgc cgccggccag cccacgcctg cgctggtcaa
    42361 aaagctggag gcgaagggta ttcccctgtc ggccgtggcc accttcgagc ggcgcctgga
    42421 cggcaaggcc gaggccctgt tccacaccgc gacggtgccc ggtgcgcggc tcgccgacgt
    42481 actggccggt atcgtccagg acgccctcaa ggcgctgccg atccccaagg tgatgcgctg
    42541 gggcgacagc gacgtggctt tcgtgcgccc ggtgcattcg ctgctcatgc tccacggcgc
    42601 cgacgtggtg cccggcaccg tgctggaact ggcggctggc cgcgtcacgc ggggccaccg
    42661 cttcatgagc cgcggcgaga tcgtcgtcgc cacggccgac gcctacgagc ccaccctgct
    42721 ggcggaaggc aaggtcgtgc ccgatttcgc cgagcgccgg gccgacatcg agcagcagct
    42781 cgtccgcgag gcggcgcgcc agacggccag cctcgggcaa tacgccgacc tgctggacga
    42841 ggtcaccgcg ctggtggagc accccacggt ctacgtgggc gagttcgagg ccgaattcct
    42901 cgccgtgccg caggaatgcc tgatcctcac gatgcgcgcc aaccagaagt atttccccct
    42961 gttcgacgcc cagggcaagc tgctcaaccg cttcctgatc gtctccaaca tgcgtctgga
    43021 agatccgtcc aacatcgtca ccggcaacca gcgcgtggtg cgtccgcgcc tgtcggatgc
    43081 ccgcttcttc ttcgagcagg accgcaaggc cggcctcagc tcgcgggtcg gcaagctgga
    43141 ttcggtggtc tatcacaaca agctcggctc cctgggcgac cgggtgcagc gcctcgatgc
    43201 ggtggcgcgg gccgttgccg tcaggctcgg cgccgacgac gcccaggccg gccaggccgc
    43261 gcgcctgtgc aaggccgacc tggtcaccga catggtcggc gaattccccg agctgcaggg
    43321 catcatgggg cgctactacg cgctccacga gggcgcccag gcggttgtcg ccgatgccat
    43381 cgagcagcac taccggccgc gttttgccgg tgacgcgctg cccgacagca acatcggccg
    43441 ggccctggcc ctggcggaca agctcgatgc cctggtcggc ttcttcggca tcggccaggt
    43501 gcccactggc gacaaggacc ccttcgggct gcgtcgtgcc gccctgggcg tgctgcgtat
    43561 cctcatggag gcgccgctgc ccctcgacct ggcggccctc atcgccgacg cggtggcgag
    43621 cttcccgccc ggcgtgctca ccgccgaggt ggatgcgccc ctgttcgact tcatgctcga
    43681 acgtctgcgc aacctgctgc gtgacgccgg ccatgcgccg gccgccgtgg acgcggtgct
    43741 ggccctgcgc ccgacccgca tcgatctggt ggtgcccaag ctcgaggccg tcgccgcctt
    43801 tcagcagttg ccggaggccc aggccctggc ggcagccaac aagcgcatcg tcaatatcct
    43861 caggaaggcc ggtggcgaat ttgccgagcc ggaggtggcg ctcctcgccg aggacgcgga
    43921 gaaggccctg ttccacgcca tcgtggatgt ggccccgctg gcccgctccc acttccagaa
    43981 cgaggcctac acggacgcgc tgcgcgtcct ggccggcctg cggtcggcgg tggaccgctt
    44041 cttcgacgag gtaatggtga tggccgaaga gccgctggtg cggcagaacc gcctggccct
    44101 gctcaaccag ctcgccgggc tgatgaacca ggtggcggac atctcgcgcc tgtcggcgtg
    44161 agacggacgg ggggattcat gaagatcatc gtgcttgacc gggacggcgt catcaaccag
    44221 gactccgacc agttcatcaa gtcgcccgag gaatggaagc ccattcccgg ttcgctggaa
    44281 gccatcgccc gcctcaatga atccggctgg cgcgtggtga tcgcctccaa ccagtccggg
    44341 gtggggcgtg gcctgttcga catggacacc ctcaacgcca tcaacgaaaa gatggtcaag
    44401 gcgctggccc aggcgggcgg gcgcctcgat gcgatcttct tctgccccca tgccgccgac
    44461 tccacctgcg actgccgcaa gcccaaaccg ggcatgttcc ggcagatcgc cgagcgcttc
    44521 aacgtggacc tgcgcggcca gccggtggtg ggcgacagcc tgcgcgacct gcaggccggt
    44581 gtggccgtcg gctgcgcgcc ttacctggtt ctgaccggca agggcggcaa gacccggctc
    44641 gatcccgacc tgccggaagg cacgcagatc ttccccgacc tggcggccgt cgtcgcacag
    44701 atcacggcct gaggaggcaa cggtggtcct gatccgcaac ctgctgttca tgctggccct
    44761 ggcggtgttc acgccgctct acgccctgat cggcgtgctc agttcgccct tcgggccgca
    44821 gacgcggcac aaattgatcc gcggctggcc gcgcatcatg ggctgggtca tcaagcacgt
    44881 gctggggatt ccctaccgtc tcgtcggcgc cgagaacatt ccccgggaag gcccggccat
    44941 catcgtctcc aagcacatgt cggcgtggga gacgatcctg ctgcaggaca tctttccgcc
    45001 catggtcttc gtgctgaaga aggagatcca cagcattccc ttctttggct ggggcatcgc
    45061 ccgcatgccg atgatcgcca tcgatcgcgc ggccggcaag gacgccctga cccaggtgga
    45121 ggcccaggga cgcgaacgcc tcgccgaggg cttctgggtg accatcttcc cggaaggcac
    45181 ccgcgtcgcg ccgggcagca agaagcgcta caaggccggc gcctcgtggc tggcggccca
    45241 gaccggtgtg cccgtggtgc cggtggccca caacgccggc gagttctggc cgcgcaacgc
    45301 cctcttcaag cgcccgggcg tggtgacggt cagcgttggg ccggtcatcg acaccaaggg
    45361 cctcaatgcc gagcagatca acgccaaggc gcaggcttgg atcgaaggcg agatgcgcca
    45421 gctcttcccg gggcactatc gcccgacccg tcagcgcgcg gccgaggcgg tggagccggc
    45481 cgcggagtga gccttccggc gtcgccgcgg aaggacggac tgcagcggga ggtcctgctg
    45541 gacggtgtgg cgatcgccta caccttgaag cgtagcgccc gccgtacctt cggcctgcgg
    45601 gtggacgcgc gggggctcac cgtcggcgtc ccgctgcggg ccacccagcg ggcggtggac
    45661 gcctttctgc acgaccatgc ccgctggatc ctcgacaaag tgcaggtcca cgcggcccgc
    45721 gccgaggcac ccccgtttgc cgtggtggag ggcgccgggt tgccggtgct cggtgaaacc
    45781 tggacgctac acctgtcgaa aggactcaac cggctggtct ggcacgaagg gggcgtgacg
    45841 gcggacgcgc cgcgcatcct cgaaatcgcc ctgcgcgccg gcagcgatcc ggcggccgtg
    45901 ttgcgacggg gcgtacagca gcgggcgctg gaggtgttcc ggcaccgggt tgcagagttc
    45961 tgcctacgga tcggccagcc ggtgccgacg gtggggttgt cctccgcgcg cacgcgctgg
    46021 ggcagctgca gccggcgcag cggcatccgc ctgcactggc ggctgatcca cctgcccctc
    46081 gagctcgtcg attacgttgc ggcgcatgag gttgcccatc tgctggaaat gaaccactcg
    46141 ccacgcttct ggtccgtggt cgagtcggtc tatcccgaac acgtggttgc ccgtgcccgt
    46201 ctgcgggcag tggcggcctc cctcccgcag ctttgatcga aggactcccc atgcgcatcc
    46261 tccacaccat gctccgcgtt ggcgacctgg aacgctccat cgctttctac accgaagtgc
    46321 tcggcatgcg cctgctgcgc cgcagcgact atccggacgg acagttcacc ttggcctttc
    46381 tgggcttcca ggacgaagcg gatggcgcgg tcatcgagct gacctacaac tggggcgtga
    46441 cgtcctatgc gctgggcacg gcctatggcc acatcgccct ggaggtgccg gacgcctatg
    46501 ccgcctgcgc cgagatcaag gcgcgcggcg gcaaggtgac ccgcgaggcg gggccgatga
    46561 agcatggcag cacggtgatc gccttcgtcg aggatccgga cggctacaag gtggaactga
    46621 tccaggccaa gaaccggggc tgaggggccg gggccgggcg aaatccccgt ccggcaggca
    46681 ccgtacggtc aggcgtccgc cggggcgacg ctgcggaagg cctcgtgcag ggctgccagc
    46741 tcccgcttca gctcggccag gacggcgggt gcgtcgctga gatgcttgtg gcgcccgagc
    46801 tgttccagct gcatggccct ggcctgggcg cggcaggcga aaaagttggc gacggcgccc
    46861 ttcagcgcgt gggcggcggc attgacggct tccgcgtcct gatgggcgat ggcgttttcc
    46921 agttcgccca ccatgcgtgg ctcgtcgtgc aggtacatgg cgatgagctg cgtgagtaat
    46981 tcctcatcgt ctcccagatt ggccagcacc gccgcccggt cgaacaccgg cccgagggcg
    47041 acggtctcgt gggccatggg ctgaggtggc gggtcgctct ggtcggcgtg atggatgagg
    47101 gcttccacca ggtccggggc gttgatgggc ttggacaggt agccgtccat gccggcgtcg
    47161 aggcaacgct cccggtcgcc cttcatggca tgggcggtca tcgccacgat gggcgtgtgg
    47221 ggggtgccgg ccgcttcgcg gcggcggatc tcggcggtgg tctcgaagcc gcccatgacc
    47281 ggcatctgca cgtccatcag gatgatgtcg aaacgctggc gggcgtgcag ttcgagggcc
    47341 tcggcgccgt tgttggccac cgtgacctgg tggccgagct tggccagcag gcgcagggcg
    47401 agggtctggt tgatggggtt gtcctcggcc agcaggatgt tgaggacgcg cttctgttcg
    47461 cgcaggctgt ggcgggtgat cagcgggcgg tcgccgtcgg cttcgccgcc cacgtcgcag
    47521 gacaggagga tcgcgttgag caggtccgag gcctggatgg gcatcggcag ataggcggcg
    47581 atgcccattt cgcggcagcg ggcgccgtca ccgcgacgcc cccgttcggc cagcaggatc
    47641 acggacaggg ggcggggcga ggcggcggaa agggcactcg ccaggtcgaa gccatccatg
    47701 tcgggcagat cgcggccaag cagggcgacg cggtaggggc tgccccgctt ggcggcggcg
    47761 aggagttccc ggtgggcggc gctggcgtca ctgaccatca gcggcgacat gcgccagtgg
    47821 gtgaggagct ccgacaggat gccccggtcg gatgcgctct tcaccacgac cagcacgggt
    47881 aggccttcaa gttcggtgtc ggtttccacg ggtaccaccg actggccagg ctcgaacacg
    47941 gcgttgaact cgaaggtgct gccctggccc ggctcgctcg tgacacggat gccgccgccc
    48001 atgcccttga caagctgggc gctgatggca agccccagcc cggtgccgcc atagcggcgc
    48061 gtggtggagg tgtcggcctg ggagaaggcg tcgaagatga ggccctgctt gtcccgcggg
    48121 atcccgatcc cggtgtcctg cacgctgaac gtcagctcga tgcggcccgc gtccttgttg
    48181 cccggtgtgg gatgcacgcg gatgcggacc tcgccgtggg gcgtgaactt gatgccgttg
    48241 gagatgaggt tgatgagcac ctggcgcagg cgatgggggt cgccgcgcag ggtgtcgggg
    48301 gtctgcggtt cgatctgggt gacgagctgg aggtcttttt cggcggcgcg ctgctgcagg
    48361 gtgcgggtgg ccagggagat gcagtcgcgc aggctgaagt cgatgtgctc gaagtccagc
    48421 ttgccggctt cgatcttcga gaagtcgagg atgtcattga tgatgttgag cagggattgg
    48481 gcggagcttt tcacgagcgt caggtactcc cgttgttcgg cgtccagatc ggtgtcgagg
    48541 gcgaggtcgg tcatccccag gatggcgttc atgggggtgc ggatctcgtg actcatgttg
    48601 gcgaggaagt cgctcttgag gcggctgttg gcctccgcgg cttccatggc catgcgcatg
    48661 gcctcctcgg cgcgcttctg gtcgctgatg tcgcgcaggt aggcggtgaa caggcggcgg
    48721 cccgagctga tggccggcac gatggccacg tccaccggaa aggtctggcc accggcgcgg
    48781 gtgacgaggg tttcgaagcg ctgaccggct tcgtcccgac gttcgggatc gaggacctgt
    48841 tgcaggatcc gctcctgttc atcgcgccgc tcgtcggaga agatgaggtc gcccacgtac
    48901 tggtgcaggg cgtccgcgcg ggagacaccg aagctgcgct cggcggccgg attgaattcg
    48961 atgatgcgtc cgccatcgtc cacggtgacg atgcagtcca gggcggcttc ggtgatggcg
    49021 cgattgcggg attcgctgtc ggccagggcg gactggccgt cgtagaagcg gatggaggtc
    49081 cagttgaggg cgtccacgag cttgcggatt tcctcaaggc ggctgggctc gttgaggacc
    49141 gcgccttcgc cggtctccag gccttcggcg aaggcggtgg cccgctcgag gctgtgcagg
    49201 gggccgcgca ggaagcgccg gatgatgacg gcgccgagtg tgacggtcag cagaccggtg
    49261 atgaggctgt cctcgacgat gtgcgccagg gcttcgcggg cggcggtcgg ggcgatctcg
    49321 aggcgcagcc agccgcgggg gacatcatcg cggatctcgg cccagacaac gatggcgtcg
    49381 gtctcgccct ggcgcgtggg ctgcgtgtcg gcgggcaggg cgaggggccg gtcggcggct
    49441 gcatcgacgc ggggcgcgcc ctcggtcacg ctcacgccgg caaccggcag gccgtgaagg
    49501 tcggtgacgg tgaggtggag catgtcgcgc tggcgggcga gcgcttcaag atggtgcgtc
    49561 agaccggtcc gatcgccggc ggtcaggtgg ggcgcgagat cgtctgccag gtgccgggcg
    49621 ctctcttcgg cgcgctgacg ttccaagcgc tccacgtaag cggcctgttc gacggcggtg
    49681 tacagggcgt agatgccgat ggcgagcccg agcaggacgg tgaacagcag ggtgagctgc
    49741 tggcgcaggg agtcaagccc gaggaggctg gcgagacggt cgaacagcgc tttcattcgt
    49801 cgcggtaacg gtcccggatg gccagcacct cgtcccagcg ctgcaggaag gcgtcgatgc
    49861 aggccgggtc gaaatgggcg ccgcgcccat ccttgagaaa ctgcacggcc cggtcgagcg
    49921 cccaggcggg cttgtagggc cgctcggagg tgagggcgtc gaagacgtcg gcgacggcga
    49981 cgatgcgccc cgacagggga atcgcctcgc ccgtcagtcc gcccggatag ccgctgccgt
    50041 cgaacttttc gtggtggctg agggcgatct cggcggccag ctggatcatc ggtgaggacg
    50101 agcccttgag gatgtcgtgg ccgatgctgg cgtgctggcg catgatggcc atctcctcgt
    50161 cagtgagtcg gccgggtttg aggaggatgt ggtcgggtgt gcccaccttg cccacgtcgt
    50221 gcatgggggc ggcgctgagc aggcgttcgc agaattcggc gccgagcccg agctgctcgg
    50281 cgatcagccg cgagtagtgg gccatgcgca ggatgtgggc gccggtctcc gggtcgcgga
    50341 attcggccgc gcgggcgagg cgggtgacgg tctcgcgttc ccgcgccacg atgtcggcgg
    50401 tggcgcggcg cacggcttcg cgcaggtcgt cgttgtagtg gcgcatgcgc aggtgggaca
    50461 ggcgcagggc gagcatggtg cgcacgcggg ggtcgaactc gtagcggtcg atgggcttgg
    50521 tgaggaagtc actggcgccg gcggtgaggc tctggtagcg gatctccttt tcgtgggcgg
    50581 cggtgaccat caggatgggc acggcttcgg ccgggtgcac cttgcgcagg caggtgatga
    50641 attcgatgcc gttgaggtcc ggcatcatgt agtcgacgat gaccagatcg ggttcgtgct
    50701 cgctgcacca ctgcaggcct tcgaggggcg aggtgaagca gcggatctcg gtgtcgggcg
    50761 gggcgatttt gcggacgagg gcctggatca gtgtgagatt caggggggtg tcgtcgacga
    50821 tggcgatggc catgcttgct ccaggaagcg ggaccgggag aggaatccgg ccagagtcct
    50881 cagattatcg gcccgttgtc ctcgaaattg aatgaacgtc cggcaggttt tgcagaaatg
    50941 ctcatgggat tttctgtgcc cccggtggcc ggggcggtgc ggggcatcgc gagggtgaag
    51001 gtggtcccga cgcccagctg gctgctgacc tcgaggcggc ctcccagcac ccgcgtgacc
    51061 atgttgtaga cgatgtgcag gccgagaccg ctgccgcccg tgcccaggcg cgtggtgaag
    51121 aacgggtcga agatgcgccc caggtgggcc gggtcgatgc cgtggccgtc gtcccggaag
    51181 cacagtgtga cgcccgccgg gccgtcggcg gcgggggtca gggtgatggt gccgtggtcg
    51241 cgtccggcga aggcgtggac gagggcatta ctgaccaggt tggtgatcac ctggcccagg
    51301 gggccggggt agctgtccat ctccagggcc ggggcgggct cgatccggag ggtgtagggc
    51361 gtgcccttga acatggggcc gagggtcgtg accacttcgc gcaggacctc gccgagttcg
    51421 aagcggcggc gttgttcgct ggtctggtcc accgccacct gcttgaagct gctgaccagg
    51481 tcgcgggccc ggctgaggct gcgcagcagc aggtcggagg cggtgcggac gctgtccagg
    51541 tagctttcca gatcgctgcg gcgcaggggg ccggccgcct tgcggtgggt cagctcgtcc
    51601 gtccgggtcg cgagggttga ggtgaccgtg acgctgttgc caaggggggt gttgagctca
    51661 tgggcgatgc ccgccaccat ggagccgagg gcggcgagct tctcgctgcg caccaggtct
    51721 tcctgggtcc ggcgcagggc ttcgagggcg ttggagagtt cggtggtccg ttccacgacg
    51781 cgcagttcca ggccctggtt gagctcttcg atgcgagcct gggcacggcg cagttcgctg
    51841 atgtccacca tcgcccacag ggcgcactct tcgtcgcgga tgcgcaggcg ctgcccggag
    51901 atgaggcagg tggcgacgtc gtcccggttg cagggccgga tgcggatttc ctgctgcgcg
    51961 agcacgtcgc tggagactac ccgggcgtac agatcgtcgt aggcgcgagg atcggtccag
    52021 cagccgaatt ccccggacgt gcgtccgagc acggtgttcc gctcgatgcc gaagaggcgt
    52081 tcccaggccg gattggcatc cacgtgggtg cgatcgccga cccgcgtgag ggagagggcg
    52141 aagggggcca ggttgaagat cgtggcgaac ttgcgttcgc tgtcccgcaa ggccttctcg
    52201 gccgcctgct gctccgtgat gtcgtggatc atgcccacca ggcagggttc attctccagg
    52261 gtgaccatgg agacattcag cagacacgtc cgccgggtct tccagcgggt gccgagcgtg
    52321 cacgggaaat cggtcacggc accgtgcaag ctgagctcgt ggagcatggc ctcgcgctct
    52381 tccgggaaag cccacagacc cagttcgacg gcctgggcgc cgatggcctc gtcgcggcgc
    52441 cagccggtca tccgctcgaa ggtcggattg acctccagca ggtggccgtc cagacggctg
    52501 atggtgaggt agtccggact ggcccggaag gccgcggaga acatggcctc ggacacgcgc
    52561 aaggcgtccg cggcggcccg gccgatgcgt tccaggcgcc gcggctcggt gacgtcgcgg
    52621 atgacggtga ggaggccgtc gcgtccgcgg acgcggatca ggcgggcgcg cagcagggtt
    52681 tccagcacac gaccgtcccg gtggcacagg gtcgccggct ggtcggagac gtcatgtccg
    52741 gcacgcaggg tgtcgaggac agtccgggcg tcggacggat tgcaccataa gccgagggcc
    52801 tcggccgtgc gcccgagggt ggcttcacgg gggtggccca gcgtgtctgt ccacccctga
    52861 ttgacgtcgt cgatgcgtcc gctgtccgga tcgctgagca tgaccgcatc gggcagcagc
    52921 cggaagacct gggagaggcg ttcctcgctg tcccgcaagg cggcctcggc ctgcttgcgg
    52981 gccgtgatgt cctccaccgt ggcgacgaag ccttggaggc tgtcgccggc ggtgatgggg
    53041 gccacgtgca ccgacagcca gccttccgtc gccccgctct gcacgacgcg ccgctcgtaa
    53101 tgggtctggc agcgcagggc gcgtgcctcg gcccaggcgg ccgagacgcg ggcggcatct
    53161 tcgccgtcca tgcgctgggt gtccggacgc gtgcgcagtt cttcgtcggt gaggccgctc
    53221 agggcccggt agcgggagtt ggtgtattga aactgaccgt ccgggctcac cacaaaaagg
    53281 cccaggtccg agctctcggc aagcgccgtg aaccggctgt gggattctgc cagcgcggac
    53341 tcggcggcga tccggcggtc catctcggcc cgcaggtggg tggtgaggcg cgcctctgcg
    53401 gcgttctgca ggcacagcgc attgatcagc agtgcgcagc cgaggccgga aagggccagg
    53461 gcggcggcca gggcgcgggc cacgctgccg tccgccagca cgcccgcgga gcccgcgagg
    53521 agcacgagac cccaaccgcc aaggcccacg gcggcgagac ggtgtccggt gcccggctgg
    53581 tgcgtggcgc gtcgccagaa gacgcgggct gcggcgaagg tgaaggtgat cgccacggca
    53641 tccaccggga gtgtgcgcag gacttccgac cgtcccggcg ccgtggggac gagggcccac
    53701 agggcggcca gcaggggcag cagcatgaca cggggcgagt gtccgtcgcc ggtgacccgg
    53761 cccagaccga tccacaaggc ggcactcatc cccatcagac cgaccggggc gaggagcgcc
    53821 agcggcaggt gggtgagcgc gggggccagc agggcagcgt cgtggacggc atggcaggcg
    53881 aaggcggcgg cccacgcccg cagggcagtc tggggacgct ccacggcggc cacgtagcgc
    53941 aggctcgcgg ccagcagcat ggaaatggcc agggacaggg ccagcgcagc ggagggggag
    54001 atggcgaaca tgcgggtcgc cgcgcggatc aggcgagcgc cttgaggagc gccgctatgg
    54061 tgtcgaagcc catgtcgtag aagcgctgga gcagcggggc gaagcgctcc agggcgagca
    54121 tgatgacgcc gaagccgacc gtcagggtga tggggaagcc gatggcgaag aggttgagct
    54181 ggggggcggc gcgggtgagg atgccgaggg cgatgttggt gatcaggagc gcagtgacca
    54241 ccggcagggc cagcagcagg ccggctgaaa aggtgacggc gccgaagcgc accagcaccg
    54301 cccagccgcc ggcatggagg ggcagggcgt tgaccggcag ccattcaaag ctgcgcgcca
    54361 gcacatcggc catcagcagg tggccgttga gggccaggaa gatgagggtg gccagcaggg
    54421 tgaggaattc gcccacgacg gcggtctggc cgttggagcc cggatcgaag aaggtggcga
    54481 aggacaggcc catctgcagg ctggtgagtt cgccgagcac gtccagggcg gcgaacacga
    54541 ctcgcatggc aaggcccatg gccaggccga ccagggtctg ctggaagatt gtcagcaggc
    54601 cgctccacga cccgagcgga atggccggga tcggcgccag ggccggtacg atgccgagcg
    54661 ccagggcgag gcccgccacc aggcgtatcc gacgcggcag ggctgtgttg ccgaacaccg
    54721 gggcgctgct caccatcgcc aggatgcgcg ccagggggta ggcgaagagg gccagccagg
    54781 cgtccagctg ggcggcggtg acggtgagca cggtcagccg atcatggcgg ggatgctttc
    54841 gaagagccgg cggatgaagt cggtgaggag ggtgatcatc catgggccgg ccagcaccag
    54901 ggtgatgaag gtggccacca gtttgggcac gaaggacagg gtctgttcgt tgatctgggt
    54961 ggcggcctgg aagatggaga ccacgaggcc ggtggccagc gcggcgatga aaaggggcgc
    55021 cgacaccagc agggtgagct ccagcgcctg gcggcccagg tcgatgacgg tggtgggggt
    55081 catggcagcg tccgtccgtg gaaggggtca gccgaagctc tgcaccagcg aggcgaccag
    55141 caggttccag ccgtccacca gcacgaagag catgatcttg aagggcaggg cgacgatggc
    55201 cgggctcatc atcatcatgc ccatggacat gaggaccgag gccaccacca tgtcgatgat
    55261 gaggaaggga atgaagatga tgaagccgat ctggaaggcg gtcttcagct cggaggtgac
    55321 gaaggcgggg atcaccacgc gcaggggcaa gtcttcggcc ttgtccacct tgggggtatt
    55381 ggcgagcttg gagaacaggt tcagatcggg ttcgcgcacc tgacgcagca tgaagccctt
    55441 gactggtacg ctggcccgct cgacggcctg gtcgaagctg attcggtttt cctgaagggg
    55501 cacccaggca tcggtgtaaa tgcggtcacc caccggcgcc atgatgaaga aggtgaggaa
    55561 cagggacaga ccgacgatca cctgattggg cggcgaggtc tgggtgccga gggcgtggcg
    55621 catcagggac atgacgatga tgatgcgcgt gaagctggtc atcatcagca acatcgccgg
    55681 caggaagctc agggcggtga gcagcagcag ggtctgcacc ggcaggctgt actgggtgcc
    55741 gccgccggga gcggggccgc tggtgagggc gggcagggcc tgggcgaggg ccggcagggg
    55801 cagcagcagg agcaggggcg tcaggcgacg caggcgggcg gcggtcatgg ggcgcggcgc
    55861 tccagagtct gccggagcca gctctggaag tcccgctcgc cggccgggcg ggacggcacg
    55921 gcgggcagat cagtggcgtc cagggtgtgg agcggggtga cacgtcctgg tgcgacaccc
    55981 aggaccagca gcttgccggc cacctccacc agcacgaccc gctcgcgcgg gccgactgcg
    56041 gttgcggcac ggacctggag cagcccggcg ccgccggcgc gggccacgcc gctgcggcgc
    56101 agcaggtgca gggtgccgaa gagcaggcca atgaccgcac cgagggcgag gagcgtctgc
    56161 accacgctgc cggtgaggtc gggtgcggcg tcaccggcca gggcgactgg accgatggac
    56221 aggagcacgg cagggacgag ggcgcggcgc atggacggac ttctggcggg gagaatcaca
    56281 gcataggacg cccgggcgac gggtcatggg aggaacaggc acgcaaaacc ggcacttttt
    56341 catgtattgc ctgcgcttca atgaactgcc gtcgcggccg ttactcaaag ccggaggtat
    56401 gacgaggagg ttcgatcatg ttgctctggc gtcgtgaggt tcaaccggtg gtcaaacccg
    56461 tgccggaggc ggtgccggtg tccgcccagg cggactatga ggaaaagcgc cagcgggcgc
    56521 tgcagatcct cggcgagcgc tggctcctgc atcgggccaa cgcgccggat cgtcgtccgg
    56581 tgcgctcggt gctgggcggc gcggccggtc ggccgctttc ctgaagggcg cccaagcgcg
    56641 atcggaacag gggagggcgc acttccgcgg gggcggacgt gcgcgggcag cgttcagctg
    56701 cgcagtttgc ggatgcgttc ggcgggcgtg atgatgtccg tcaggcggat cccgaacttg
    56761 tcattcacca ccaccacttc accctgggcg atcagggtgc cattgaccag cacgtccatg
    56821 ggctcgccgg ccaggccgtc cagttccacc accgagccat gggccagctg gaggaggttg
    56881 cgaatggcga tcttggtgcg tcccagttcc acggtgagct gcaccgggat gtccaggatc
    56941 atgtcgaagt cgttcagggt gccgctcttg gtgccggcct gaccgaaatc cgggaagagc
    57001 tggctggcag gtttggcctg atagggcgat tcgtccacac cggtcgccgg atcggtgagc
    57061 ccttcggagg cggcctgctc ctgcatggcc gcggcccagt cgtcatcgct gacctggttg
    57121 tcgtcggggg cgtcggtttc ggtgtctgac atggcttact cctgagcctt ggcgggcggg
    57181 ctgtcttcgt cctgggcgag gaagcgctcg accttcaggg cgtactggct gcgctggatg
    57241 ccatagcggc attcgaagat gggaatgccg tccacctcag cctggatggt ttcggggatg
    57301 ttgagcggga tcacgtcgcc gggcttcatg ccgacgatgt cccgcagggt gacgtgggct
    57361 gtgcccaggc gggcgacgag ttccacctcc gccgtctgca gctggcggcc catcatcccg
    57421 acccagcggt tgtcctgggt gatgtggtcg ctctgcatgg tcgagtacag cgtctcgcgg
    57481 atgggctcca gcatcgagta cgggaagcag aagtgcattt cggcctggga cccgccgaac
    57541 tccagcgtga aggacgccgc cacgacgatt tcgctggggg tggcgatgtt ggcgaactgg
    57601 gggttcatct ccgagcggac gtactcgaag tccagcttct gaacggggga ccaggccttt
    57661 tcgtattcgt tgaaggtgac cttcagcagg ccctggatga tgcgctgttc ggtgggcgtg
    57721 aagtcgcggc cttcgacgcg ggtatggaag cgtccgtcgc cgccgaagag gttgtccacc
    57781 accaggaaca ccaggttcgg gtccagcacc accagcgcgt tgccgcgcag gggcttggcg
    57841 atgaacaggt tgaggttggt gggcaccacc agattgcgca cgaattcgct gaatttctgc
    57901 accttgatcg ggccgaccga gatctccaca ttgcggtgca tgtagttgaa caggccgatg
    57961 cgcaggtacc gggcgaagcg ctcgttgatg agctccatcg tgggcatgcg cccacggacg
    58021 atgcgctcct gcgtcgccag gttgtagtcg cggacgccgt cctggctctc ttcgccgctg
    58081 tccggctcgt cggtctcccc ggtgacgccc ttgagcaggg catccacctc ttcctgggag
    58141 agaaagtcct gggacacggc cgcgcttcct tactggacga tgaactggtt gaagagcacc
    58201 gccagtaccg gaccgtcgtc ctcggggcgg cgtttggcct tcttcttggg gttgggggcg
    58261 tagccgagca cgtcgttggc ctcgtccttg acctcgtccg ccagttcctc gcggccctcg
    58321 gcattggcga gctcggaagg tcgtttggac gatagcagca tcaggatgcg gtgcttgagt
    58381 tcgggcatgt aggccttgag ggagtcgccc accttggcgt cagcgacgcg cagcaccatg
    58441 gtggcctgca ggaagtgttc gccctcttcc ggcgcgagat tgacggtgaa ttggtccagc
    58501 tgcacgaaga ccggcggctt ggacggatcc accttgacct tgggcggctc gtggtgggcg
    58561 ggctcttcct cttcctcgcc ctccgcgtgc ttcttcttgc cgagcaggaa gaacgcggcg
    58621 ccgccggcga tggccaggat caggaccacc gcgacgatga tgatgagttt tttcttgccg
    58681 ccgcccttgg cgggggcttc gccttcggcg gcttcggcct tggccggtgc tttcgacatg
    58741 ctggatcact ccttttagtg tctgattgtc atcctgcacg gacgcccgga aagcccggaa
    58801 tagcggcgga agcgccggcc atttgtgcct ttcaggcgaa cgtgtccacc aatccttcgc
    58861 cgcggcgcag ccagggaccg gccgaaccgc cctggacgct gccgatgcca tctacgccgc
    58921 gggtgccgcg gccggcagtg ccgcgccgtg ggccgtcacc accctcgccg ttgcccgact
    58981 ggccggacgt gcccacggag gtctggccga gggcgatgcc ggcatcgccg agcagctcgc
    59041 gcaggcgcgg cagggcctgc tcgatgagat cgcgggcggc cggcgtggcg gccacgaact
    59101 gggcgctggt ctggtcgccc gatacggaga cggtcacttc gaccttgccg agctgcggcg
    59161 gcgtgagggt gagttcggcc tggctttcgt gctggccgac catccagctc acccggttgc
    59221 tgacctcttc cggccagccg gcatgggtgg caggggtgtg caccggcagg ctcagggcgt
    59281 tgctggcggg tgtcggggct tcgccgcgga tctgggccag ggcggcggcg tgcaggccgg
    59341 cgaagctgtt ggcgggggcg gcgtcggtgc cggcgggcag gcgtggggca tcggcgctgc
    59401 ccgcatcggt cggtgtggtt cggaacgcgg cggcgaattc cggcaagcct gcggcagttt
    59461 ttgccggcga tttgccggtg gtgtcggccg gagcgctttc ggccggcgtc gtcgggcgcg
    59521 gatcaatcgg gggcgcgagg aggccggcga gggccgctga cggcgactcg gtggccggtg
    59581 cgtcgtcgtc ggtgcgtgca gccggtcggc cgtctgcctc gcttcggcct gtcgcctggc
    59641 cgaggaagag ggcgagtccg aggcgctggg gcgcttccgc ggacggcggc gcttcgccgg
    59701 gcggtgccgc gggcgcctgt gcggcggctg tgtcgtcgcg ggtcttcgat cctgcgctgc
    59761 ccgcctcgtc ggcgctgcgt ggccgatcat ccgcctggcg atccacttgc tgcttgaggg
    59821 cagacccgaa atcgccgtcg gcggatgccg atgcttccgg cgtgcgcgcg tcgccgctac
    59881 gggaggcgga ggcgttgccg cttggggcgg gcggggcgac ggtcgcgatg gggttcaggc
    59941 tcgtggcagg catggcgggt tcctggtgag gatgcggaca ggcgatccac tgcaatccgc
    60001 atgccatgcc ggtcccggcg gggcgttcag ggctcgccgt cgtcgtcggc gtcccggtcg
    60061 cggaaatgct tggcggcgtg ttcgtcggtg agccgttgct cctgtttcag ttccttgcgg
    60121 agctgggcgg tgtgatggcg ctgggacaag gtgtcaaagg ccttgacctt gttgcgctcg
    60181 tcccgccatg cctgctgacc ctgttcggtc cgccgccggg agtcggaggc cagcttctgc
    60241 tgctgggcga tcgcgtcgtc gagccgggcg atgaaggcgc tgtagttgcg ccaggcgtcc
    60301 gggccgatgc cgttgcgggc ggcttccatg aagcgcgcct ggtattcgtc gcgatagtcc
    60361 ttgagcaggg cgagcttctc ttcgaccgcg tgctcgctgg caatcagttc gcccaggcgg
    60421 cgcgcagcct cgtccatgcg ggtgttggcc agatccagca gaggcttgag ggggaacggc
    60481 tcagtcatct tcacgttcct ggggtgctat cgggcggggg gcgggaatct tgagggtcgg
    60541 aggcattggg cggttcagga cgtgccggcg aacagggctt cgagtttttc gacggcgctc
    60601 tcgtagggct cgctctcctc gatgcgttgc tgcaggaagg cttccaggcg cgggtagtag
    60661 gccacggcct ggtcgaggat ggggtcggag ccggcctggt aggcgcccac agcgatcagg
    60721 tcgcgggagc gctggtaacg ggagaagagc tgcttgaagc gccgcaccac gtcaaagtgg
    60781 gcgggcttga ccaggttgtg catcgcccgc gagatggact gttcgatgtc gatggccggg
    60841 taatggccct ggtcggccag attgcgggac agcacgatgt ggccgtccag gatggcccgc
    60901 gcggagtcgg cgatgggatc ctgctggtcg tcgccttcgg ccagcacggt gtagaaggca
    60961 gtgatcgagc cgcccccttc ggggccattg ccggcccgct ccaccagggc cggcaggcgc
    61021 gcgaagaccg aaggcgggta gccgcgggtg accggcggtt cgccgatggc cagggcgatc
    61081 tcccgctggg ccatggcgta gcgggtgagg gagtccatga tcagcagcac ctgcttgccc
    61141 tggtcgcgga aatattcggc gacggtggtg gcgtaggagg cgccctgcag gcgcatcagc
    61201 gggctggtgt cggctggagc ggcgatgacg accgagcggg cgagcccttc cggtcccagg
    61261 ttgtgctcga tgaattcctt gacctcgcgg ccccgctcac cgatcaggcc gacgacgatg
    61321 acctcggcgg cggtgtagcg ggccatcatg ccgatgagca ctgatttgcc cacgccggag
    61381 ccggcgaaga gccccatgcg ctggccgcgg ccgacggtga gcagcgcgtt gatggcgcgg
    61441 atgcccacgt ccagggagcg ggagatgggc gcgcgctgca gcggattgac cggccgtgcc
    61501 tgcagggagc gggtgtgctt gacgtccagc gggccgaggc cgtcgagggg ccgtccggcg
    61561 ccgtctacca cgcggccgag gagggcgtcg cccatgggca ggtgcttggc ccggtcggag
    61621 gcccggcggc gcggttcgat gcgctcgccg gcgatgagct ggggctgggt cggttccacc
    61681 ggcaggacct gggcgccggg ggcgaggccg aagacgtcat cggtgggcat caggaagatc
    61741 ttctcgccgt ggaagcccac cacttcggcc tccaccgtgc cgccgcccgg caccacgatg
    61801 cggcagccgg agccgagggg cagcttgagg ccggacgctt ccatgaccag gccgttgatg
    61861 cgggtgaggc gtcccgagag ttgcagcggc gaggtggcgt tgaccagctt gcggctgtcg
    61921 tggaggaagg cccgccaggt ttcgaggccg tggtccatca gccttcgccc tcccagggga
    61981 tgttgcggcc caggttgtcc atgatgcgcc gccagcgggt cgggacggtg gcgtccacct
    62041 ggctgccgcc gccctcgatg cgtgcgccgc cgcgggcgac gccggcatct tccacgaggc
    62101 ggtggccggc gtgggtgagg agttcgccca ggtagtcacg caccatgccc aggtcttccg
    62161 ggttgagatg gatctgggcg tgggcctggg gcagctggac gagggcctcg cggatcaggt
    62221 cgacgacgct ctccgggcgg gtcgcgaggg tctggcggac catcaggcgg gcgacctcca
    62281 gggacagggc gaggacctcc tcggccacgt ccgcgtcgag gcggctcatg gccgcttcga
    62341 ggccttccac gagcccgtgc agacgcatgg cctccatgcg accgcgggcg gtggcttcct
    62401 cgtaaccggc ggcatagcct tccttgcgcg cctcctcgtg aatgcgctcg atgtcttcgg
    62461 cggtggggag ctggatgtcc ggttcgggca gaggcagcgc cgcctcggcg ggggaagctt
    62521 cgaccggtgg cggttcggac ggatcgggcg ccggggcggt ggccgtcacg gctgcgtcgg
    62581 ggggcggctc gccggggggc ggcgccaacg cttcgggcgg cggtgcgtcg aaggagggga
    62641 agtcccagcg acggtaggcc ccgctcgctt ggtggcggtt cagggccatg gtcggccccg
    62701 gggcttacac gaaggcatcg tcgcccccct tgctggacag ggcgatctgg ccttcctcgg
    62761 cgaggcggcg ggcggtcttg aggatctctt tctgctccgc ctccacttcc gacaaacgca
    62821 cggggccgcg ggattccaga tcctccttga gcatctcggc ggcgcgctgg gacatgttct
    62881 tgaagacctt ttcgcgcatc tcgggcggtg cgcccttgag ggcgatgacc agggagtcgc
    62941 tctgcacttc ccgcagcagg gtctggatgc cccgatcatc gagatcaagc agattctcga
    63001 agacgaacat ctcgtcgagg atcttctggg ccaggtccgg atcgtattcg cgcacgttgt
    63061 cgaggattgc ggtctcggcc gcagagccga cgaagttgag gatgtcggcg gcgtggcgca
    63121 ccccgcccat ggcggtcttc ttgaccgagc tggcgccggc gagcatgcgt gtgagggctt
    63181 cgttgagctc ccgcagggcc accggctgga cgccgtccag ggtggcgatg cgcagcagca
    63241 cgtcgttgcg caggcggtcg gtgaagtgct tgaggatctc gccggcctgg tcgaattcca
    63301 ggtgcaccag gatcgtggcg atgatctgcg ggtgttcgtt cttgatgaga tcggccgccg
    63361 tcggggcgtc catccacttg aggctctcga tgccggcggt gtcggagccc tgtagcacgc
    63421 ggctgatgag gtgggacgcg cgctcgtcgc cgagggcctt ggtgagcatg gccttgatgt
    63481 gctcgtggtc ggcctcgatg ggcgagcctt tttcggccag ggcctccacc tcgtcgagga
    63541 tggcctccat gcgctcccgc gactgggcgg gcatggcggc catggccgcg ccgagcttct
    63601 gcacttctcg cggacccagc agtttgagga cctcggcggc ctcgtcggcg ccgagggtca
    63661 tcagtaacag ggcgctcttc tcgaggcctt catcgctgct cattgacgcc catccattcc
    63721 ttgatcaggt tggcgaccac gcgcgggttg ctgcgggcca gatcgcgcac gcgggcgagc
    63781 ttttcctcgt aggcgaggcg ggccgcggct tcgggggaga gttccacctc ggcgccttcc
    63841 tcgccctctt cgcccggttc gcctccgcct tcctcgccgg cttcgcccgc ggattcggcg
    63901 gacgcctctt cctcggcctt ctccgggggc ggcggcatga cctgcttcat cagcggacgg
    63961 atcacgccga aggcgacaaa ggcgatgacc ccggcaatga ccaggtactt gagcagctcc
    64021 ttgaacaggg cgatgttgtc cgggtccttc cacaatgggg tttccggcag atccttttcc
    64081 gaggcgacga aggggctgct cgccacgctg agggtgtcgc cccggtcctt gctgtagccg
    64141 acggcgtcgc gcaccagatc ctcgacgcgc ttgcgttcct cctcggtgag ggggaccggc
    64201 ttggcgttgc cgttcttgtc cttctcttcc ttctggttga gggccaccgc caccgacagg
    64261 cggcgaatct ggccgagggc cttgcgggtg tgctggatcg tcttgtccag ctcgtaattg
    64321 atggtggcgt ttttgctcgt gctgaggggg agctggccat tggcgcccgg tgtgcccggc
    64381 acggccggcg tggtgatggg ggcggtggcg ggcaccggcg gctggttggt gagggcgccg
    64441 ggcacgccct gggcgccggc gtcgcgggtc tggctctcgc tgctctgctg gctgcggatg
    64501 gcctgttcgg ggctggggtt ggggcggtag gtctcggcgg tctgctcggt gacgttgaaa
    64561 tccacgtcgg cggtgacctg ggcgcggaag ttgcccttgc cgaacagcgg cgtgaggatg
    64621 tcgttgatgc gctggatgta gccctcttcc agctcgcggg tgtacttgat ctgggtggcg
    64681 tcgagaccgg cgcgcatggg gtccggcttt ttggtgagca ggttgccgtc ctggtcgatc
    64741 acgctcacgt tgtcgttggt gaggttgggc acgctggagg ccaccagatg cacgatgccc
    64801 gccacctggg aggggtcgag gctgcgggac tggtgcagat tgaccatcac cgaggcggtg
    64861 ggcttctgct cgtcccgcag gaaggcggtc tgcttgggaa tcgccagatg cacgcgggcg
    64921 ccggccaccg cggcgatcga cgagatggtg cgggacagct caccctccag tgcccgctgg
    64981 aaattgacct gctcgtggaa ctgggacagg cccagcttct gggtctccat cagttcgaag
    65041 cccaccaggc cgcccttggg aatgccctgg gcggcgaggc gcaggcgcag gtcgtggacc
    65101 tgggtgctcg ggacgaggat ggcgttgccg ttgtcggaga acttgtaggg gatgttctgc
    65161 tgctgcaggc tggcgacgac ggcgccgccg tccttctcgg ccagattgga gaagagcacc
    65221 gcgtaatccg gctggcggct ccacagcagc acgcccacca ccagggcgat ggcggcagcc
    65281 agggcggcgc cggcagcgat cttctgacgc tggctcaggg cgcggatgtt ctccagcacc
    65341 tgggtgaagg gcgatggggg cggaacgggc ggggcggcgg cgtcttcggc tgccatggga
    65401 acagtctccc ttttacggat gcacgcattc tgggcgcccc gtcctgccgc cgacggcgga
    65461 attagcgacg ctttttgccg cctatttgcc gcctaggcgt gacaagggcg ggcataagct
    65521 gcgagccggt gaaattcgtt tccgtgtgcc catgatcccg accgtcgccg aagaccgtct
    65581 cgacgcgcgc cagctggccg aggccttccg gcagttcagc gaggcttccg aggccctgac
    65641 cggcgcctac gccgggttgc aggcccaggt cggccagttg accgaacgct tcagcgtgct
    65701 gatggaggcc ctgcccgcgg gggtggtggt gctcgatcgc gccggcgcgg tgctgcaggt
    65761 caatcgcgtg gtggagcgga tgttcgccac ggtgccgccg gggaccctct ggagccattt
    65821 ttccgggtcc cggctggtgg ccaccgagac gccgggcgaa tgggccgtgg catgcggcga
    65881 cggaccgcgg cgggtgaccc tgcaggaggc gccgctggaa tccggcgagg gcggcatcgt
    65941 gctgatcaat gacgtcaccg aggcccatcg cgcccgcctg gcggccgaac gtaccggccg
    66001 gctcgctgcc atgggcgaga tggtggcggg gctggcccac cagctgcgca cccccttgtc
    66061 ggcggcgctg ctgtacaccg cgaccctggc ccagcaggac ctgaaaccgg gcgaccggga
    66121 caagatcggc ctgcgcgccc tggagcgcct gcgccatctg gaaaagctga tccgcgacat
    66181 gttgctgttc gcccgcggcg agaccctggg acgtgaaccc tttccgatct gcgacctggt
    66241 ggcggaactc gcccataccc tggagccggt ggcgcgcgcc aaaggcatcg cgctggcggt
    66301 cgagtgcgat tgtggccgta cggaagtcgt gggtgatcgc aaggccgtgg ccggcgccct
    66361 caccaatctg ctggaaaacg cggtgcaggt gctcgagacc ggcgggcaca tccgcttcga
    66421 cgccggtgtc gagggtgctc atgcgtgctt ccgcgtggcg gacgacggtc gcggcatcgc
    66481 tccggaatta cagtcacggc tgttcgagcc gttctttacc acccggcctg acggcaccgg
    66541 tctgggtctg gccatcgcgc gaggggtggc gcgggcccac ggcggcgaca tcggcatcgc
    66601 gtccgaaccg ggccggggca ccaccttcac actgaccctc gccatgggcg cctgaacgga
    66661 cgcccggcca tccgaagcag tgagagatca cccatgaacg atgccctcca catcctggtc
    66721 gtcgaagacg atgcgcccct gcgggacgcc gtttgtctga ccctggaagt tgccggccat
    66781 acggtcaccg gtgtcggcga cggccccgcg gccttggcca ggacggacga cgaggccttc
    66841 aacctggtga tctccgacct caagatgcag cccatggatg ggctcgagct gcttgccgaa
    66901 ctgcgccgcc gcctgcccca gctgccggtg ctgctgatga cggccttcgg cgatgtggag
    66961 aaagccgtgg acgccatgcg gggcggggcc tgcgatttcc tgatgaagcc cttcgagccc
    67021 caggtgctgc tcgaacacgt gcgccgctat gccgcgccgg accgggaccc gggcggcatg
    67081 gtggcggccg acccccacac ccgcaagctc ctggccctgg cagccaaggt ggccgagacc
    67141 gacgcgaccg tgctcctgac cggtgaatcg ggcaccggca aggaggtgtt tgcccgttac
    67201 ctgcacgagc agtcgccgcg ccgcaacggc cccttcgtgg ccatcaactg cgcggccatc
    67261 cccgacaccc tgctggaagc gaccctgttt ggccacgaga agggtgcctt caccggcgcg
    67321 gccaccgcac aggcgggcaa gttcgagcag gctgcgggtg gcacgctgct cctcgacgag
    67381 atttcggaaa tgcccctggg cctgcaggcc aagctgctgc gcgtgctcca ggagcgggag
    67441 gtggagcggg tcggcggcaa gaaggcgatc gccctggaca tccgcgtcct ggccaccacc
    67501 aaccgggaca tgaccaagga ggtggccgcc gggcgcttcc gcgaggatct gtactaccgc
    67561 ctcaacgtct ttccgctggc gatcccgtcc ctgcgggagc ggcccggcga tatcgtgccg
    67621 ctggcccatc actttgcccg tctgcacggg gcgcgcctgc gtcgcagcgc gcgcctttcg
    67681 cccgaggcgg agtcggtgct ggtgagttac cactggccgg gcaatgtgcg cgagttggaa
    67741 aacaccgtgc agcgcgccct gatcctgagc agcggcgagt ccgtctcggg cgagacggtg
    67801 cgtctgtgtc tgcctcactg gaacggggag ccgctacgac ccgtattgcg cccggccaca
    67861 ccggtgccgg cagattttgc cggtgaggct ttgccgggcg gcaaggaatt gccgcgtccg
    67921 gccaacatga aggatctgga gcgggaacac attctgcagg tgctgcggga ggtgggcggc
    67981 tcgcgcaagc gggcggtgga gcggctcggg atctccgagc gcaccttgcg ctacaagctg
    68041 cagcagtacc gggaggaagg ttttttcccg ggatgaggtg cattgccctg cgtggcatgg
    68101 gtcttgctgc ggtggagagg cattgaagga gccggaacat ggatacccgc ggagtcgatc
    68161 agatgttgtc ggagttgcgc gccacggcgc agacggccgc gggcaagcct gcccaggcgg
    68221 ccgcggcgac cgggcaggcg gatttcggac aggtgctgca gtcggcgctg gatcaggtga
    68281 gctccgcgca gatgcaggcc caggagatgg ccaagggctt ttcgtccgag gatcccaacg
    68341 tgaacctgca ggacgtgatg gtgaacctgc aaaaggccaa cctgtcgttc cagcagatgg
    68401 tgcaggtgcg caacaagctg gtgaccgcct accaggacat catgaacatg agcgtctgag
    68461 cccgcctgga aggctcgtcc cccacgtgat acagccggcg actgccggct gtcttcgttt
    68521 tggggggctt cagcgaatcc ggtgtcgggc gttgagctcc cgctccgtgc gcaggatgta
    68581 gcgctggatg gtggtggaca tgttgccggg caggccgagg aactcgcagc ccgcccgtag
    68641 catcacctgg ccgttggggt gggtgatgcg gaacaggttg cgcaccttga tcgccgtgac
    68701 gatggggccg gtctcgggca acatcaggcg gcagtcggcg aactccatct ccggctcgaa
    68761 ggccaggccc tgggggggca ccagcacggc aatgccgcca ccgctgatgt cgatgacgct
    68821 ggcttccacg gagcgggggc gggcactctc gccctcggcc accgggatga ggcaggacag
    68881 gctgtgggag atcggcgcgg tgagacggta gtactcccgc cgctgcaggc gcagcaccac
    68941 gtcgggcatg cgcacacgaa aggcttcgtg gccctcgaac ggaaaacgga cgatctcgcg
    69001 ggccgcaaac tggatcttga tcttttccag ctgggcgacg cagatgagct gttcggcccc
    69061 ttccacgcga cggttgaggc tgtcgtcggc tgcggcgtcg aggatcaggt ggcgttcgtc
    69121 cggggtgacg gcgaggaggg cggtcagaaa ggaatccgcg ctggcgtcga tgaaggcgct
    69181 gatcatcgcg cgcttctgca ggaggccacg gaggttgaag aggatgtcgg tcttttcccg
    69241 cagcaggtac tgggaataat cgtcggcctg caggagttcg aacttgagcg gcatgatggc
    69301 agggatccgg tttccgggag gggcaatgat agccggtcgg gccggctctt ccggtaacgg
    69361 caaattccat accaacgtaa ggccggccaa caaaaaaggg aacgtcgccg ttcccttttc
    69421 cggtgcgtgg gcggcgtgct tcagcgggtg gcttcgcgct ccacttcttc gaggctggtg
    69481 tggcgtacgt cgcggccctt gaccatatac accacgtact cggcgatgtt cttggcgtgg
    69541 tcgccgatgc gctcgacgga cttggccacg aacatcatgt cgatggcgtg ggagatggtg
    69601 cgcgggtcct cgatcatgta ggtgatgagc tggcgcatga tgcccttgaa ctcggcatcc
    69661 acgtccttgt cctgacgcac cacctgggca gcggccacgg tgtcgaggcg ggcgtaggcg
    69721 tcgagggcgg tgcgcagcat gtccaccacc gcgtcggcaa tgtggccgag gcggatctgg
    69781 ggcatgaagg ggctggcgga ggcgtggatc tgcttggcga tcttggcgat cttcttggct
    69841 tcgtcgccga tccgctccag gtctgtgatg gtcttctgga cggtgctgat catgcgcagg
    69901 tcgaccgccg ccggctgacg cttggcaatg atctggctgc agaattcgtc aagttcgact
    69961 tccaggctgt tgacgcgggc atcgttctcg atggtctggt cgatgaggag aagatccccg
    70021 tgggcgagac cttccatggc cttgacgatc tgcagctcga ccatgccgcc catctgcagg
    70081 acgcgggtcc ggatgccttc aagctcggcg tcgaacttct tggaggtgtg ctcgttcatg
    70141 gatgtcccgg ctcggtgact gatagacgcg gaaggctatc agtgcaatat gacagatttg
    70201 tttcaggcgc gttcgaaggt ctggacgccc tgcgcggttg ccagcaacag cacgtctgcg
    70261 ccgcgcttgg cgaacaaccc actggtgacg actccgacga taccatcaat ggcgatttcc
    70321 agtgtgacgg ggtcgtgaat gttcaggccg tgtacatcca ggatcaggtt gccgttgtcg
    70381 gtgacaaagc cctcccgcag cacgggacgc ccgcccagac gctcaagttc gcggctcacc
    70441 tgggcacggg ccatggggat gacttcgacg ggcagcggaa aacgtcccag ttgcgccacc
    70501 ttcttgctgg catcacagat gcacacgaag cgctgcgcca cggcagccac gatcttctcg
    70561 cgggtctggg cgccgccgcc gcccttgatc atggccagcg tgccgtcgat ctcgtcggcg
    70621 ccgtcgatat agaccgggat gtcggcgacg tcgttcaggt ccagcaggcg gatgccatgt
    70681 gcggcgagcc gcttggcgga ggcttcggac gagggcaccg cgccgacgat gcgctccttg
    70741 cgggctgcca ggccgtcgat gaagaaattg gcggtggagc ccgtgccgac gccgacgatg
    70801 gtgccgtcct gcacgtagtc gagggccttc agcgcggcca gctgcttgag ttcatcttgg
    70861 gtcatggcgg atgctcaacg gtaaaagacg gattttaacg gctcggaggg ggccctgcag
    70921 gcgtcagatt ctgtgtccac ggcatgtggg tcagcggtgt gggatagagt ggccggcacg
    70981 cagcccccgg cccgtgcacg gcgcaggcgc cggagtgggc ggctttcctg aggaggtacg
    71041 gacatgggtt tgctcgatca attggcgggt caggtgctgg gttccctggg gggtggccag
    71101 ggacagggcg gttctgacct gcagcacatg ctgctccagg ccgtgttggg cctgatccag
    71161 aacggccagg gcgggctggg aggcctggtg gcgcagctga accaggcggg cctcggcgaa
    71221 caggtggcgt cgtgggtcgg gaccggtcag aacctgccgg tgagcgccga tcagatcagc
    71281 caggccctcg gtggccggct cggagacatg gcggcgcaat tgggtctgcc tgcggatcag
    71341 gtggccggac atgtggccga gatgctgccc caggtggtcg atcacctgac ccctgacggc
    71401 cagatgcccc aaggcggcga tctgctttcc gcaggcctgt ctgccctggg cggcctgttt
    71461 ggcaaggcgt aaggctttcg gcgttacgag cgccggcgtc agcccggtcc ggtcgcgacc
    71521 ggccgggccg gatcggcgca ccattcgctc catgagccgg catacaggcg ggaatccgtc
    71581 aggccggcta cttccatggc caggatgttg tggcaggcag tgacccccga gccacactgg
    71641 tggatcaccc ggtgggcatc cccgcccttg atgagggtgc cgaactcttc ctgcaattgc
    71701 gcggccggtt tgaagagtcc gtccgggccg aggttgtccc gaaagaagcg attgatcgca
    71761 ccgggaatgt ggccgccgac gggatcgagg gtctcgtttt cgccgcggaa gcggtcaggc
    71821 gcacgggcgt caatcaggca cagggccggg tcgtccagat ggccgtgcac gtacgtggca
    71881 tccacatgga gagcgggttg cagccgcggt gaaaacacgc gggcggtgag tgcagggacg
    71941 tcctggctga gggcgcgtcc actgctggcc caggcctgga tgccaccgtc cagtagggcg
    72001 accgtcggat gcccgagcca gcgcagcagc caccacaggc gcgcggcaaa catgccgcca
    72061 gcgtcgtcgt aggcgaccac ctgggtgccg gtcgtgacgc ccaggctgcc gagcttggcg
    72121 gcgagcagtt gcgggtcggg gaggggatgg cggccgttga gacccgagag cgggccggag
    72181 agatcttctt ccagatcgag atacacggcg ccgggcagat gtccgctcgc gtaggcgtcg
    72241 cgtccatagc gacggttgcg cagatcgaag cggcagtcga agaggcgcca gttcgcgtcg
    72301 gcctggtctg ccagttccgc ggcgctgatc agcgtgccga aagtgccgtt catcgtgcgc
    72361 gaccctcact gcaccggcga ttcgaccccg gccagccagg cgcgggcgtc gtccgcatcg
    72421 tcgaacacgc ggacatctgc gttgacgaag agctgcgaca gccaggcgct ccagctgacc
    72481 cattgatcgc tggtaacgac ggcaatctgg gcgaagtcgt tagcgtgggc gcgggcgaag
    72541 cggatctctt cgagggccat gtccacggtg aagccgctca tggcgcgcag gtcgaagaac
    72601 agattgaccg ggccctcgaa gcgcaccgtg tagttgaaga cttcctcgaa ttccttgtag
    72661 tcggagaggg tgaattcgcc gaacacgacg gcgtccacct ggcctgcgtc gtgggtgatg
    72721 gtgatcatcg ccggatcctc agagtcaatg gagaaagggg aaaaaaccca tcgccagtgt
    72781 agcccccgac gcagggggct tcaatcggat tccgcgacgg gcgcgggttt gcgcgaggcg
    72841 agggagacca gtacgccgct ggcgacgatg agggcgatgg cgatgaagga ggtccacggc
    72901 aggcgctcgt cccacaggag ggcgccgaac gcgctggaga agatcaccgt ggtgtaggcc
    72961 agattggcgg acacgatggt cttgccgtag cggtaggcgc gagtcatggc gagctgggcg
    73021 cagcccccga atccaccgac acccaacacg atggccaggc cgctggcgtc gaggcggtgc
    73081 aggtgatcgc tgcccagcgc ccagggcaga ccgaaggcgg cggtaatcag ggaaaaccac
    73141 aggacggtgc gggcttcggg ctctccggcg cggccgagct cgcgcacatt gatgtaagcc
    73201 agactggcga ttaggccgga gccgagtccc cccagcgcac cgggccactg gtcgggttgc
    73261 agggtgggct tgaggagcag cacgacgccg acaaagccaa gggcgacggc cgcgtggcgc
    73321 agtgggccga cgggttcgcg gaaccacagg gcgaggatca gcgcgacgaa gattggcgac
    73381 gtgtagttga gtgtgacggc ggtagcgagg ggcaggatgc ccagggcgaa gaagtagcac
    73441 atcagggcca cggtgcccga cagaccacgg ctgagctgga gcttccagtg gggcgtggca
    73501 aaagggatgc cttgcacgcg cagcaggaca gccatcatca acagcccgat gaggccccgg
    73561 tagaacacca gctcgcccgt ggaaaagcgc aaggcgccga gcttgacgca tacgcccatg
    73621 caggcgaaga gaaagctggc agcgatcatc cacagggatt gcatggcggg gatgggagcg
    73681 ccgggcagcg cgtgggaggg ggacgtattt taacgggcga cgttcgccgt ccgcgtgggt
    73741 caacgaaaaa acccccgcgg gcgcaaaccc gcgggggtct tcaaccaggc gtgccggaga
    73801 gggagtccgg cgatccaggc atttcttctt agtggaactg ttcttcttcg gtggagccgg
    73861 tcagcgcctt cacggaggaa gagccgccct ggatcacggt ggtcacgtcg tcgaagtaac
    73921 cggtgcccac ttcctgctgg tgggacacga aggtgtagcc cttttcgcga gcggcgaatt
    73981 cgggttcctg gaccatctcg acgtagtgct tcataccttc gccgcgggca taggcatggg
    74041 cgaactggaa ggtgttgtac cagttgacgt ggatgccggc cagggtgatg aactggtact
    74101 tgtagccgag ggcagacagt tcttcctgga aggaggcgat ctggctgtcg ttcaggttct
    74161 tcttccagtt gaaggacggc gagcagttgt aggacagcag cttgccgggg caggcggcct
    74221 ggatggcctg ggcgaattca cgggcgaagc cgatgtcggg cacgccggtt tcacaccaca
    74281 ccaggtcggc gtacggagcg taagccacgc cgcgggagat ggactgttcc agaccgttct
    74341 tgacgcggaa gaagccttcc tgggtgcgct cgccggtgag gaagggcttg tcgttctcgt
    74401 cgtagtcgga ggtgatcagg ttggcggctt cggcgtcggt acgggccagg atcagggtcg
    74461 gaacacccat gacgtcagcg gcgaaacggg cagagatcag cttttcgatg gcttcgcggg
    74521 tcggcacgag caccttgcca cccatgtggc cacacttctt ggcggccgcc agctggtctt
    74581 cgaagtgcac gccggcagcg cccgcggcga tcatgttctt catcagttcg aaggcgttca
    74641 gcacgccgcc gaaaccggct tccgcgtcgg ccacgatggg caggaagtag tcgatgaagc
    74701 cttcgtcgcc cggattgacg ccacgggacc actggatttc gtcagcgcgc ttgaaggtgt
    74761 tgttgatgcg gcgaaccatg gtggggacgg agtcgtaggc gtacagcgac tggtccgggt
    74821 acatggtttc ggaggtgttg ccgtcggcgg cgacctgcca gccggacaga tacacggctt
    74881 ccaggccggc cttggcctgt tgcatggctt gacccgcggt gatcgcgccg aaggcgttca
    74941 cgtagccctt cttggcggcg ccgttgacct tgt
//
LOCUS       DAOQPA010000004        73117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_116_length_73117_cov_19.879281, whole genome shotgun sequence.
ACCESSION   DAOQPA010000004 DAOQPA010000000
VERSION     DAOQPA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 73117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73117
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_73117_cov_19.879281"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PKX01_01170"
     CDS             <1..1554
                     /locus_tag="PKX01_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HMZ74758.1"
                     /translation="PLPPQVPSAPSTDLTVRPPASPAIATPAGPRVTLRDITLSGNTL
                     LDSTTLQAGLGPVSGQSFDLAGLEALAAGIERQYRAAGYPFTQAVLPPQDLKDGTLRI
                     QVVEGRYGRIQATGTDPLPASAQPFLDFGLRSGDAIENARLERTLLILDDQPGMKIRP
                     VIQPGDRTGEADLSVNVERVSLVGGEVGLDNTGARSTGQNRARGAVFINSPFRYGDKI
                     SINALATDQSMLLGSLDYEMPIGASGLRGQVGYAYTNYQLGAQFAALDAKGYAKITTA
                     RLSYPLIRSQASNVLLSAGYQHKNLEDRYDSTGLIRNKHSDGTPVAIQFDRRDTLLGG
                     GVTYGSLTWLPGSLSLDPGMDALDRVTARTAGRFSKLNLDVARIQQLTSSVHAYARYS
                     GQWADKNLDSSEKFNLGGFYGVRAFPLGEGVGDQGWFTQLEIRYDVGVVTPFAFYDAG
                     HVETNAKPWDVNSASTRRLAGSGVGVRSLRDGWSLDATLAWRTQGGAPTSEDVDRNPR
                     LFVMIGRRF"
     gene            complement(1599..2498)
                     /locus_tag="PKX01_01175"
     CDS             complement(1599..2498)
                     /locus_tag="PKX01_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ74759.1"
                     /translation="MKRRTTAGVVLAVLNTLGAVSAARAGEFDCMIEPRKTVDVRAAS
                     EGLIEKIWVDRGDMVKAGQVLVTLDSGVEKAATESARYRATMEGKIRTGESRVEFATQ
                     KYNRRDKLAGQSFVSMQDRDESLTEKRLAESELIEARDDRKLAEIEYRRLSEQLRLRT
                     IKSPVDGVVVDRLLHAGELADNRDLRKPILKLADIGTLHVEALLPVDAWGKLAVGQSI
                     EILPESPVGGRYTATVKVIDRVMDAASGTFGVRMELPNPGLKLPAGIRCKASVPGVTG
                     RTVLAPPKKPAAAPTAPGAPASH"
     gene            complement(2501..4510)
                     /locus_tag="PKX01_01180"
     CDS             complement(2501..4510)
                     /locus_tag="PKX01_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ74760.1"
                     /translation="MSRAVLSTWGVHAFAPGLYPERQDAKPGKLDRFMGGLLGRWARH
                     GSALRLPPVRRFAEQVIAHEGELAALDEATLQARVAPLRAALAASGLDDANAAEVFAL
                     VREVCFRRLGKRHYPVQLMGGYTMLNGGLAEMMTGEGKTLTAVLPAVAVALAGVPVHV
                     ITVNEYLAQRDSEQLAPVYGFFGLSVGLVTPEQEPPERIVAYGCDVCYCVNKDLVFDY
                     LRDRITAQRIKAGARALLEGWLGRSAPVGGSGTLLRGLYYAIVDEADSVLIDEARTPL
                     IISAEQDDPNGRAFYEQGLAVARSLNADEHYRLHLKERSIELTEAGRAAVAEASRGLP
                     GLWQVSRAREELVEQGLTAEHLFELDRHYILAEGKVQIVDEYTGRVMADRSWEKGLHQ
                     MIEVKEGLELTKRRDTISRITYQRFFRRYLRLAGMSGTVTEVAPEMRTVYALDVLRIP
                     PNRPVQRRDLGLRVFARSEARWDAVLASARAHAAAGRAVLIGTRSVAASELIGARLAA
                     AGLDHEVLNARQDAREAEIVADAGQPGRITVATNMAGRGTDILLTPEVKAAGGLHVIL
                     TEYHDSARIDRQLYGRSGRQGDPGSYEALVSLEDELFVAHAARLVAWLRVRTAPDGEV
                     ARRFGPLLRRFAQGAAEAQNARIRRHTVDSDNRMEQALAFAGRGE"
     gene            complement(4503..6650)
                     /locus_tag="PKX01_01185"
     CDS             complement(4503..6650)
                     /locus_tag="PKX01_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HMZ74761.1"
                     /translation="MAGSFLSASWYRVAGLRPQLRGHAQVQRQRFRGKPWYVMRDAAS
                     GKIHRFTPGAYAVLQRMDGRSTLDVIWQDVAAAAGVDAPTQDEVIELLSKLHSGDLLQ
                     TDLPPDVAELAERGARQKRQKRLQSFLNPLSIRLPLWDPDPFLNRSWPWVARLFGPLG
                     VILWLLAVVPAGVLVGMHWDELTGNMGDRLLATDNLVLLFFIYPAIKFLHELGHAWAV
                     KSGHGEVHEMGVMFLVFAPVPYVDATASGAFRSKWRRALVGAAGMLVELFLAALAVML
                     WVLAEPGFVRSVAYNVIFVAGLSTLVFNGNPLLRYDGYYILADLIEIPNLAQRANQYW
                     QWLAKRHLFGAVEVERPAATPGERRWFLFYGPASALYRLFVTISITLFIAGEFFFIGV
                     VIAIWGAITMFGVPVGKMLGYVWGSAELQRHRPRALWVTFGGLAALLLFLFVVPMPLR
                     THAEGIVWVPAQAELRAKGNGFVERVLAAPDAWVQAGDLVIVTRQPDVDADVTQRRER
                     VRQLEVQYAAQMFDDRLQAAVVAEDLGRERAALARAEERLDELLLTAGVSGRLRLSRP
                     DDLPEHFVKKGELVGYLLAGPPRVVRAVVTQDDIALVRDRLEAVEVKIADRLAETYPA
                     QVSREVPGAQDQLPNRALAMEGGGLHATDPRDPDGLKTLERLFQFDLALPDSVGEVQL
                     GTRVYVRFIHRAEPLADQWGRRLRQLFLSRFHV"
     gene            complement(6663..8489)
                     /locus_tag="PKX01_01190"
     CDS             complement(6663..8489)
                     /locus_tag="PKX01_01190"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ74762.1"
                     /translation="MSAAPADAAVPVAPDALWGAFVSADNAADFCKSWLALQCSQVGD
                     VRAGLLLLEQGGGTFVPAGVWPTDQTDVTYLAPGAQRCLSERRGTLQRGLEGALVVVA
                     YPLEVDGRIHGAVVLDVAERPDAALQDVLRRIHWGAGWLESVFLRRQAQDEALALTRA
                     RAALDVLGVAAEQARLEDLALAVANDLASRFACDRVAFGVDRAGRARLVALSHSAFFE
                     KKSQFVVALENAMDEALDQRRSVIWPTDTDAAAGGIAIAHRDFAAQRAVCSVLLSSRG
                     QGIGVLTFERNAGEPFEPDEVLGFEAIGALLGPLVESRIELHRWFAGRLADKVRDGWR
                     HLRDPRRPAFRAAVTVIAVLLAAVVLIDGDYRVSAKSVVEGEVQRSVVAPFEGYIREA
                     PIRAGMLVKKGQMLARLDDRDLLIERQRYSAEREQHERRYRDALSKHERANAHVSLAQ
                     MAEAESQLALVEEKLARAEVVAPFDGVVVTGDLTQLLGSPVEQGKLLFEVAPLDAYRV
                     ILKVDDRDIRYVRAGQGGRLVLTGFAGDALDFKVHNISMAQAEEGKNLFRVEARLDRG
                     DIPLRPGMEGVGKIVVGERSYAWIWTHRLFDWLRLQAWNWLP"
     gene            complement(8599..8883)
                     /locus_tag="PKX01_01195"
     CDS             complement(8599..8883)
                     /locus_tag="PKX01_01195"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMZ74763.1"
                     /translation="MSDNAAVPETPEGQRIRMNTASLKSTYCNMVNATTTREEVVLNF
                     GVNESWDRTGGDYEVKLEHRVILSPFAAKRLSQMLSKLVDEYEHRYGELK"
     gene            complement(8923..43995)
                     /locus_tag="PKX01_01200"
     CDS             complement(8923..43995)
                     /locus_tag="PKX01_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HMZ74764.1"
                     /translation="MAFEPLEPRVLLDGTPSVPITRIDGSIDVAGETDRYGFTLSNDA
                     RIVFDSLTNSNSFTWSLSGPRGDVVSSRAFAQSDADYAQPVLQLAAGDYTLSVDGVMD
                     TTGNYAFRLIDLNAARAIDAEVPVTATLETGYQTDAFAFDAAAGDRFVFDNTGDTINA
                     TWRLIDPFGAQIGSNHGFGDFDSGSLAAAGRYTLLVEGRIGAAADATPYAFRVLKAGE
                     KVSTLTPGDVTRGTLASAAQSARYDFTLGETGQYFFDSLTADSRLTWSLRGPLGQTVS
                     PRSFYYSDAYYSTGALALGPGDYSLYVSASSDFSGDYAFRLANLTQATPVNFDVDTAA
                     TLDPANGSLMYSFSGTAGERIALQQTGSGPSQTVFWRILGADGQQIAGVNYFGGFMEL
                     PKLADSGTYTLIFEGDVTASGTTDFSFRMIQCPDVTEAIALGDVIDRSIDTPGQVDHF
                     TFSLASATRAVFDSLSNDYNLSWGLYTADGRLVAQRGFAYSDGWSSGDGLLDLAAGDY
                     QLVVDPYADFTGPFSFRLLDTSAGSAISLGDTVTVDVGHSGRDTTVYNFDGQSGDQVE
                     FNFTYLSGEGGAVYSLFDPYGRRVLGATDGSPQQSRLELTGRYTLLVEGNVFSNNDSS
                     YELFLNYLSNDGGNTLPVGTAINVGDEVTGAVDDLATPVVYNFTLNSETILLFDALGE
                     DEYALNWSLTGPRGEEVSHRAFYTSDGGYYYYGLPPGQLIAGDYALTLYGPANQAFDF
                     RLVDARSTAAPLAFGTETSGTLQPGNAVQVYSIDATAGDVANLVVNSSWNGGLFVYDP
                     DGNLVVGPTYAGSQSGISFSRTGTYLLVLSGPRWQTWDTYYDFRLNLQSSVINDIQLG
                     DGVTGTADSSGNHQRYRFTLTEPQRVLFDSRTGNSGLYWNMSGPQGVERSDTNFYYAD
                     GYLYGNSPVLTLNPGTHFIDVWSTSGASADFAFSVLPVSAAKPIDVNQMVSGTLDPAN
                     GIAAYSFDGQAGDQLNLSALSSSQPYYNMAWRLIGPSGQQVASYYFYNFTPSALPESG
                     KYLLLVEGNNSLSGQLNFSFQVNRPVVVPPVGITLDTTINGNHGTTAQNDRYSFHLDQ
                     STRVYFDYLSGSTNLNWSLAGPRGTIISQRGLYSDTADYSGPASIDLAAGDYIVTISG
                     GATGAYAFRLVNEENKPVIAYGDVVKSQLSPGSEMEMFSFDAQAGDQVFVDTKPGTSQ
                     YSSYIRLIDPFGRQVASGYYFSDIDTVKLAYTGRYSVIVEGLYYYSGNATDYAFSVEK
                     VSSTSQAIDVNGTTGLDPVYVAAPDGDALVLGPYGSAQRANDPALNLTGSLTLEARVK
                     VNSFANGWMPLIVKDDGDSNHRTYSMWINSGGYVLLTSSDQYYDQSVSTNSGTIKLGE
                     WVDIAGVIDRTSGQLRIYLNGQLAVSGSLRTNAAFSHGGPLLIGATNENWNGWANFDG
                     AIDTVRIWNGVRTQTQIDTDRSTAPAPDAAGLVADYRFAASGGSTLVDSSAHGLNGAV
                     VNTLTGMPGTIVGRIDRAGGSAAYTFTLTGPRTFYVDTLLPSRSDFRWSLSGPSGLLV
                     NRAAFTDTEQIVTLDAGDYSLVVDGDGDVTGGFALRLMDLSATTAITYDQVVSGTLTP
                     SSGATAYTFNAVAGDRAYFDALSNSTGNIRWRLLDPYGNTAFSYRGLSDVDTLTLGFT
                     GAYTLLVEGYNGDMTTASYSFNVRRVVDSVTPLVIGQSSGLDPRWVPGKSGNAVQLDG
                     YDWVEAPATDALNLTGSSTTEFWFKADAYSGTWTPLVFKGNGDSNQRQVTVWLNNAGY
                     LHLSTNTNGTWQGVSTNVGSVQLGQWEHVAAVIDRIGNRLKVYINGTLAVDGGLAGGS
                     ALSNSKPLMFGSALESYPRFTGSLDDVRIWNTVRGASDIATDMSTALSGNEAGLVAYY
                     RLDESAGRSVADTSSQNSPATVRHILQSLSGTAVEGAITTPGQQVRYTFTLASAKRLA
                     MDSFTTNSNIYWTLTGPRGTVISNRTLSYSDAAEIGGTSPSMDLGAGDYVLTIDGYAD
                     TTGPFAFRLMDLNAGSNLTYDTPVSGTLDPGSSNTVYHFTGTSGDRLFFNATSLTNVG
                     QTPYVRLLDPRGQQVWSNYFSNIDVFSLAATGDYTLVIEGSAYQTVPIGYGFTLYRVT
                     DDKTSLTLDSTVNGTLAIPGQTDQFSFHLAQDTRVYFDSLTANSSLTWTLAGASGTVV
                     SRNFYYSDSDGLGGTNPFMLLRAGDYTLTVDGSGNTTGAYAFRLLTAASAEAITVGTE
                     VNTSFDPYASHLYSFDAAAGDQVMLDFLDRTSSFYPIWRLFDTDGQQLFYNYLYNDSG
                     TLTLPKAGHYLIAIEPRIYDGSGTARFQVTSQGHVDLPAPPVGDPITLGAQVNGNLTS
                     GTDSKTYALDLSDARRLYFDVLSNTPYVQWSLTGPSGALVTSRYFYSSDGSNASSYGN
                     TPILDLAAGHYTLTVGGSAGAFAFRLLDLVAATAVTLDSSGKADVSGQLSPRSETDAY
                     SFTATAGERYYFQRLQGNYNSAFWRLIGPSGQVVMGPSDQWYDVENVTLPEAGTYTLL
                     IEGPYYYTDTANYSFRISRITDPAPVTIDLGQVVSGSIDSAGQVSHYTLVLDQARQVY
                     FDTLGSANDGAVAWTLSNSLLGSVVNSRAFSQSNAYDRSGNGVLLDLAAGTYDLVVDG
                     AGSFTGAYKFRLLDTTAAATTITLDSTVVANVDPADSTRFYRFDATAGQRVFFDMLGS
                     TGSTPYWRVIGPHGEVVQDVQWGGSDRDVTTLPSSGSYLLMVEGVRNATGTASLSFNL
                     RTVTDKTDTLTLGSTVNASIDAPGQTSRYTFTLGDRKRVFFDALNYNGNVTWSLTGPR
                     GVVVSSRAFGSSDGTTNQPWLDLPAGDYQLVFDAPGDSTGAFQFRLLDVAQATAITPG
                     TVVTGVLSPVAEMSLYTLALQAGDRIYLDSLGVDRNAYDLSWRLLDPYGRIVASPGGF
                     VDVDVTTAAFSGNYMLIVEGQPAAVGTANYSFNISPAPLSKVVPIAIGDQPGADLVVS
                     NLAVTPVSSDVHSGGQVVLSWDTVNAGTLPTSGSWKDQIVVRNSRGELIYTQLVDHDE
                     AVDGPLASGAKQSHSLTLTLPDGNRGAGTLSFRVTTDVENTVAELGSGGAAELNNAAT
                     VSVASTLSPYADLQVANLNVSPAAGWAPGSQVTLSWRDVNAGVGATSGGWTDSIVVRN
                     LSTGRTFYVGSLAYDATANGDLGAGDGRDRQLTLVWPADAQGIGRFEFSVVADAQGQI
                     FENNANDSAESNNTAALTVTSAPDLVVGNLRTTPSSTESGSSVTVQWDTVNQGLAGTL
                     GSWVERVTVVNPATGETLVDTRLTYDPSVANAGNIAAGANRNRSYTFTLPEGQRAVGA
                     LNVTVSLDYNGQLVESDETNNSANVVLNVAKKKYADLAPVSLEAPPSARGGDTVTLRW
                     TVTNQGDAPTGTQWTDRIVLSADGIYGNADDVVLVTAPHFGSLAPGESYTQERQVVLP
                     QRVDGAYKLFLRTDVNAEVLEPDTRANNLFGPKAINIAAPHADLVVEAVSAPTAAQSG
                     DSVKVSWRVRNTGDSTTDSSQWKDYVYLSSDNQLDASDILLAQVDHTGQFLAVGDSYT
                     ASANVFLTYGLTGNYSFIVRTDAENRVFEANFETNNTTAALQTTKVTPAPVPDLQVSA
                     ITLPAQIVSGQTAHVAWTVNNGGGGTAKGPWTDRVYLSTDGTLNSPTFLGDVAHTGNL
                     DAGGSLSSSLDFIVPALADGNYRIVIVTDVNGQIYEGGSGSAGENNQFAATVPVALVH
                     PDLVPLQLDAPSTAVSGNRVTLTYTIRNDGTGAAGGDWIDRIYLSRDTTVDASDTLLA
                     TRAVSGPLAAGQGYGGSVDFDLPLGAQGTYYLLVAADANNQLPERNAEGNNVASRQLD
                     VTLDQYAQLVTTDVQAPTLTIGDPAQITVSWTVANQGTGVGRTSTWTDALIASTDSIV
                     GNSDDKVVGEFVHTGGLAVGDSYSRTETFLLPAYFQGRYHFFVKTDAKNAVFENGNEA
                     DNAKESPQIFDVMSIPYADLVVSAASAPTTASSGQTMSVTWTVANTGIGPTDIPGWND
                     EVVLTSDAAGNNVVANLGSFTHLGVLAAGDSYTRTQDVSIPNGLSGSYYVRVRSFGPF
                     EFVYTQNNTRVAGPVQIALSPSPDLQVTDIQTPSTANEGDYIDVKWTVTNNGAAKADG
                     SWTDMVYMRKVGQPNDPGIYLGTFSFDAGGLDAGKSYTRTERFKLPARTEGVYQAVVV
                     TNANANSGGVYEYGTAAANNTTADDQILQVALLPRPDLQVGTVVAPSSASAGAAVSLR
                     FSIINQGTVPTATPHWKDNVYLSLDNKLTGDDLLIGSYDNGAALNPGEQYATESGSLQ
                     IPIRFRGDVFLIVQADGSGSVDEYPNDANNITAVPLYVNPYPLADLVTGGVVAPAQAV
                     EGANIEVRYTVANKGSGTTASNSGAASWTDTIWLSRDKTRPNAGGNSAVMIGTVQHNG
                     ALKVGESYDVVTNVNLPSHLTSGTYYVTVWSDSYDVILEDTLASNLNPDDPHELDNNN
                     YKARAIDIIGGFEPPPPDLKITALTASPQASTSSDLTVNWTVTNAGAGEASGKWYDVF
                     WLSDKPDLNASGAKQWYLGSILHDGGTAPGASYSASATFALNPAAAGKYLIAYTDFDP
                     YVGKPYDVVEGNEDNNTRVTDTSVTAVPADLKVVTIAAPSENFSGEKTTVTWTVKNYG
                     GDVWSGTRYWLDSVYLSPDPVFLPERATALGTFVHSNKDGLAAGASYTQSADVVLPRG
                     IGGNYFLYVITDANPYDPRFPNGETDQGDNAYFRDVYYPGSAYEGVGGEQNNRGIGNI
                     PVTYREPDLKVTGLVVPPDAQSGKTITVTYTVTNIGNRDTRENAWWDRLFLSKDGSLD
                     TYDLQLAEARHYGILKTGDSYTGTATFKLPDGISGNFSLLAFTDSEADARLSWWNSNI
                     VPEYRGVGIDANSDSVPEFRGEGNNITVAPLPVVAAPLADLQVTTVTTQAHITVGNDF
                     TVTYTVANLGGADTPPEQAEWEDYIYLSRDPLLDLDSDRYMTTVRHVGGLAKGASYTV
                     NQTLRAPLDVTGAWYVFVVTDPARGSGRGKVFEGGLERNNATPSTPAIFEMPPPSDLQ
                     VDDIVFGGNGQSGENIHMEWTVSNHSTNPTSTSWTDAVYLSDDNTWDIGDRLLGKVTH
                     TGVLGQDGSYTASLDALIPPTKPGQYRVIVRPDIYNEVYEGPYRSAGEANNFTTSPNV
                     LNVTVNELHLGVYEDTTLSTGQSRLYKLTVGQGQTLKVSLTTGDQDAANEIFIRYGDV
                     PDGFHYDATYDNPLQANQTAVVPFTKPGDYYVLIRGHAETQANTPVRVLAEVVPFAIT
                     SVHVDQGGDSRWVTFDVRGAQFADNAILKVVRPDVAEYEPVKWDVIDSTWIRATFDFT
                     NAPLGLYDLEVINPSGDKAIIAYRFLIERAMETDVTIGLGGPRVLAAGETGTYGVALQ
                     SLTNVDTPYVRFTFGVPEMGNNQYVYNLPFLKYYNDLRGQPDTGGADVPWASLDSATN
                     TTGQILSSGYAYDVIAGGYAGATFNVTTYPLLKALSTLNWDALRVGLYEMYPDLEKIQ
                     ALAGGPESMQTALPAYWDLWQQANSEDGLPDDCVIPFIPYRFNIVGAATPMTRDEFIA
                     DQTTEALKLRAAILGDTDLDAKIAATGTDDTTLAEKRAAIALRNLASDADGWVNAYLG
                     ALEQVGILRPVDEAPPIRQNTKVMSLMSTLATGILLGPVGDQIRTTGDLVEFFAKIKT
                     WYGDTPKTLAAIDHYEHRESDCLEGDIPVPALPKYADYNLNLSHPTYFETFNIFVPYV
                     GFNKDGASAATIALPDYASVDASNQLTALDFSRLYAQAISDSQLASISGPQGYGVDQF
                     LPVGQALPYTVKFENPAAASSSVTEIRVASALDPSLDPTTFRLGDIKLGDVTIHIPSG
                     RALFQGDFDLRNSKGYILRVSAGIDPTTSTASWVFQAIDPDTGEVLQDATRGLLLPNA
                     ADGRGTGQLGYTVQPKADLPSGTQISAQAVVRFNAIAPQETQKLTQTLDTQAPVTTLT
                     ATPVTAGGNDYEVRWSSADEDRGSGVAHVTVYVAEDGGDFKIWLRQTTDTFGVYSGKA
                     GHTYEFLALATDNAGNREQPPGSVQAPDDGSGANLGGLPSFGETTQDLPPPPAPATPP
                     TNPLFIEAQSQTPAATPLSKLPEFTEVERPFTAQAFATDFVSSGAGVGALAMLELPDG
                     SVLASGGPNRGWLYRIPHDGGKALTPFVELDDPIYDLALDDNGQLWATTGGNDLLKLD
                     LATGAVLARYGTGITQALAIKAGTGEIYVSSGDGIEIFDPVKATFRHFSDVRVDDLAF
                     APDGTLWGTTWPDRGDVVTFDKRGRATVQVRLDGEVDSIAFGQVGTRLEGLLFISANS
                     TKGEQGAALTMVDLVSKRSVEIARGGSRGETVVATKDGRVLVAETHQIDVISPIVAPR
                     VVATNPPDQAVVTLPLTTLTVTFDQDMYAGSATDSASVLDPAVYQVVRQNGSLVDIAS
                     VAYDATTRRVTLTVAGLDPDLYDLRVLGTVRSSAGMALGGTYISSFVAVQDFSSVVSL
                     QFDRVRSDRGTGVVSYDITVTNTADYDLLVPAALVIDPARFFQGRPMGAVSPQGANGL
                     WYIDLSGKLPGGVLKAGASIATFTVGITNSASQQISLGHGIYAVPYPNQKPAFTSTPV
                     TAATAGQAYRYQAVAQDPDGRVITYLLVDGPAGLTVDSTTGILSWLPNVASPATSTVT
                     LRAYDTRGGFDTQVFNIEVAGANHPPNVSDLPAEIDGKEGQSLLVGLAAEDVDEDFLH
                     YYADNLPPGAVFDSQLNALVWTPDFTSAGTYTNVKLIVSDGKSTVTRSFNIVIAPDDA
                     PPVLSPVPERTLREGDPVRLQLHATDADSGDTLSFYADVLPAGAFLDPNTGVFQWTPS
                     FIQAGDYSLKFGVSDGTKRTETTVKFHVLNVNAAPVFDELGSWISFEGQTLAFRAFAF
                     DPDNPGYVAQDRLPSGTLTELEGAAATVSYTVSGLPDGASFDPVTSMFKWVPRYDQAG
                     SYLVHFTATDDGNGTGTPLTSDIVVPITIRNANRAPEMAEIPTQTVQRDAVFELPVTA
                     TDPDGNPLTLTVKGLPAFATFTDNGDGTGRFVIAPHSGDRGDYEIDLTATDNGDGQGA
                     LYAQSLTRRFILTVVSASEAPVIAPIGDKLALVGQKLSFYINAKDLDQDALGFTADAA
                     PAGLTITPQPQYGRALVEWTPDSTVLGQTFTVHFKVTDNGNSGATAPNTTEQVVRIKV
                     RDSNAAPVLSPIGNLSLKEGQPFSLQLSAVDPDGDPLTYSVTNLPAGASFDAVKGLFT
                     WTPNIFSAGDYPNVRFAVSDGMAESAETVTISVQNTNQAPILVSLFPQTGREGAQVQF
                     QLTVGDVDGDPVVFKALTALPDGSRFDSRTGLFKWTPTYDQAGDYSFTFEARDPAGAT
                     SQTTVSLAIANVNRAPVLTVSNHVALLGRPLSFQLGGSDADSGTTLTFSATGLPEGAT
                     LDPTTGVINWTPGAGQAGDYLVMASVSDGMTSTALPLVIRAALTAPAPSVIIENTPSF
                     PAVPGQDVLVHVIAESFGNIAGLRLFADGVELPLDEFGRAHVQATQVGKIALRAEATD
                     VDGLVGSTTSVIRVRDPQDKSAPTPSFASQLAFARLTDLTAITGSTGEANVDAWTLEI
                     ARYGDEHYTTLASGDGAVNNAAFMQLDPATLANGMYTLRLRVTDLGGRRGEASVDVEV
                     DSATKTGRLQRNEADFSWSVGGHTLDFLRSYDSLNTSVAGSFGNGWRLSFRDVDLQTD
                     VALTGREDAGELGTFQDGQRVVLTLPSGERAAFTFVPQKHSIPGQTWYTPAFQAESAS
                     GWQLVVPDTKLQRAGSSYYDLVSGVGYNPANSGDTDYILVAPDGTRYSIADGRIDDVR
                     FTDGVHVAVSDSGLVASTGETVQFTLNHAGAIERAALSDGRQYLYDYDAQGRLVSVRN
                     LEAATSLRYGYEAGALGRLTLVTGSGAGALYTYGPSGVGQAPISGDLGSSVNYLATPV
                     NGSLIAGEVDHYAFTLRPSELASTASGSVYLGIVVEGHNGLAPVLPVMPGLTAIASSV
                     VGNRAFALFKLDAAGLAQIDVAGSGVGDYTLSLFVAGDANRDGKVDGADAIAIAAAKG
                     AVRGGAGYSEALDADGNGVIESGDLQLLMQNAGFTPNRAPVVVAGTVKTHVDLDVNAP
                     VSTFVKDPEGDRTFFTLLGSTHGTAHLSGDGQSVVFTPEAGYIGTADFTIRADDGFGQ
                     TIQTIAVTISNAALTRLDFVDRNLRLAVGEGRQISLIGDFEDQANVELPASYVTLMST
                     DGAIGSLVAGGRINAVGKGSGVLIAERAGLRAATAFTVGVPTDTVQAYLYAGGMTVYP
                     GAVSLAENVGKRQILANLYGAMDLAPASSGTHYYVSNDQIISVSADGLISARQSGEAT
                     VTVINGGAETIIPVKVEKPFVGPTVLGDLGGIVQGADGSIVAVAPGALRGNTTVSIEA
                     VKAADLPAGMPDGMALAGAFKLDLGDRKLDTPIQLAIPTTGIAAGTKVVFMRYAEIPM
                     PDGSKQPIWLQEETGVVGADGYARAMSPPYPGVTVSGTYTCGIAPDASFSQVRGQTYA
                     TFPVGINDGGSSFAMLSSLGGGAAMGAAFGLTAGFMLTCLIGYHELQIISVPAQGLPT
                     VTKVGVQVDPGKVSDNRIEVNNPYSPSTSPATEPFVSSARFLFKDDGSGNVVPFVEIK
                     GDRFTYDNPAAPAFAKKGSQIADLKVVFETPDGHTFEVSPSATSTDGDVFVEVPQTVS
                     IGTAKIFVRRPIYYANGGTWDDLEQLDSNKVQLSYDARYIFGGMSGNQVFAIDSQTQD
                     LVARINVSGNFPAPRAMAISSDNTRAYVTLRYNGAVALVDTIALQQVDVDGDTANGIS
                     SIPLLPGAQPFWIAIAKGGKKAYVSDEVAGRVYVIDIDPSSSTYNTLVKTIDVNPAPS
                     GLRGMAINANGKRLFVAAPDKALFGRPGGGDGKVFVIDVEEGSSTYGQVVATLDAGSE
                     PYGVSATNDPNVMMYTNRLSDSKGVVIVKGDVGNYTQTTVTLTLGAITDWFDVNNASS
                     VVLSKDTKYAFVVGYNTYIQDVPSHDPNYDPNHPAGGNIGIIRDPLGPNPTLVGATRE
                     IPMSWPDNAVLSPDGNTLYAAFRGQRVVMAFDVAKMIAVVESGSPDLGTTAVDDIDPS
                     IDVRANFKMLKKGGLFTDPVFGVPPGATNGPIGVGFNQGLVTQGGASLELLAPDASTS
                     NLNPNFSWMVKGFEGAKARLYVSTFRGSDGLFPTDLGSTDQNPNRIFSSDLLDASSAD
                     STGAIFIKQLPANLKLTAGQQYYWGVEVVSPSGTVVARDWRVFKTEAFKDPSKPYSSV
                     TVLTHGFQLSPTQPANLADALSSFYQMAETIAKDGGEGVAARYQKSSGHWIIFYDGAN
                     SGALVGHDLVDALRMPSLNGRPIALVSDWYDESDFPTDGFSEAAADTFYSSLAQLNEE
                     SGGKIFQSPIHLIGHSRGTVVNSELAQRFGVYAPTVSLDMTALDVHDFNQPSLDIPIA
                     QYIQAAGAAAGAVSGLVGNLISLWGMLPTNAVIPTGNFLDPDVQRWSNIAFADAYYQV
                     VASNTPMGFGQIVTTPNGRYVPNFDYSVNLTGRAGFTRDDGLISASLLASGEFRAGGP
                     HSRVWRWYGGTMDLSATTFQAGGEPVYRRLYDKTKTEFQGVTVPWYNAQSFALGSGPW
                     EGIGTGWAFAPQGGVTYSGPMSATKVSLATDNTEAPNPSGMAVPSIFNGDFEEGALNR
                     SWLLQYSAGGLATRLTGDLPGWSFHGGSFAKTRTLIDGDRLVTLADGTHAAMLDNDNY
                     RIEHNRMVVPDWAEFLTVDAKVLTGKAGEKLLVRGYLADSSVPIELGSIALDNTGTNF
                     QSYTFQVPTAFRGAGRLLEFSMSSGATADAEVLLDHVQFTKGLITDSTLDPSDKAVFF
                     TDTTTGPTTGLSSQWVDLTNPFSQAFTVTVTTDPNSFLVVAGTSGDVVLNDNNAHDPL
                     YTDYVLAPGAHLKLNLKSKFNADFIKTYKDADIPIQKTNLYFTITLANGSIRDEAVDL
                     FYMPDYADGNATDGLWTFMDTMDGKTRTLSFDNAAHVELKETSDTYNLFGVSQSGGIV
                     QGLTYTGRYVNALPDFATYQVEEGGRKLGTIKVAGYSVPKQTVGVSLATLRTMVQNIR
                     TEAENYKNNPLTAVVHDPTGFAAGKYNIFLNLFPDAVITDAQWQDVVDGFNEWFGTGA
                     SAIYQNNIAQTEFSYGETGLNPIVMINQPWVNTVAKPDPNDDASRSFAGLDFAYNTFE
                     STLTKAGAGAGSFGGMVIRDDISIASKRYLLSRVVNPERAGAMGGQSAMTLVVDGILR
                     AYLSVGFTRKDMGRSYGWVVGHELAHNMGLFDEYIYSPFTNAPRDGGANFMSNANNIR
                     VSSSQAAALHLAMDNPDTTVALSSTDALLSWYLDLDALDKSNRAGRQVLNLSPLAGDG
                     EGSTDPLGGLLYFVSQNSDATSPVTSSPILISGASHDGIVNGQFSVADPAATDYGWIT
                     EGDAKVSAGAGVLTEDPRLASRFAQTFVVPADAATLRFQLVSSHFVANGDGPPDAFEV
                     ALIGADGKPVGGTLPLSGSDALFNLQADGTVYAADQVSAAGLTNRNGGTFPSGRPITV
                     SIDLTGVAAGTTVTLYFDLLGFGEAASSVTVDNVRLLKVGEVEDQPPVANADTAAVDE
                     DASVLVNVLANDTDPEGKALTLVSADGATHGAVTLENGQVRYTPSANYFGADSFTYVV
                     RDEAGNTATTSVAITVRPVNDAPVANPDSVAVLSGGSVLIDVLANDTDIDSPVLTITG
                     ATGASHGTVAIESGKVRYTPDAGYAGGDSFTYTITDGDGGNAGSSVTVDILVHNTPPV
                     ANADTAVTDEDTPILIDALANDTDAEGQALSLVSVGLATHGTVSLDAGQIRYVPDADW
                     FGADSFTYVMQDSVGAQASATVNVTVNSVNDPPVANPDSATVEAGQSVLIDVLANDTD
                     KDSAQLAVTAVSGAAHGTVVIESGQVRYTANAGFTGADSFTYTVSDGDGASVDGSVAI
                     TVQLHNTAPVAHADSAVTDEDHSVLIDVLGNDSDAEGQVLSLVSVTGATHGTLAIEGN
                     QVRYTPDADWNGADSFTYVVADSLGATAKGAVDVTVTPVNDAPTLDALADAAVKEGDS
                     FTVTAVGHDVDAGDVLNYALDAAPQGAKIDPLTGALTWKATDGDAAYAFTVRVSDLSG
                     GTTTRSFTVNVANVAPTLQAGGLPAVYAGQSYTLELSSSDPGDDTLSLWHINWGDGQV
                     QDVAGNPGQITHTYDGSVLGQVQIRATATDEDGSYALDPLAIAVLPVPLQVRSFTSDS
                     NGFAVRFNDPFDASVINLYDSTLSNLGVSDVVLSGSGGSLVKGSLVFDADGKGLRFLA
                     TGSGLAADTYSLVLKSGPLAFHSAFGALDGNGDGTGGDDYTTRFTIGPVPALRLSLPD
                     FVRGPGQSVDVPATGKYLPLSLSSAGDVRNLSFTVRFDPSLLAITGAVAGAGLPSGAS
                     LNVDTSVAGQITVTIASATPIAAGKLTLVNLVARVPDTAAYGAVEVVDIGNVFANQTA
                     ADRADDDALHVVGYIGDGNRNAKLDKDDVTLLQRNSLKLDSGLAAWSYIDPLLIGDID
                     GDGKLTTADASRVAQKIAGVARPEIPDVPAGLKVNFATAPTPPTAPVIDFGSSFAGFT
                     VGSSDPKFKKENWKKAFVTNMATSSTNPNANLKVTVDASVQSTTPA"
     gene            complement(44338..44871)
                     /locus_tag="PKX01_01205"
     CDS             complement(44338..44871)
                     /locus_tag="PKX01_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HMZ74765.1"
                     /translation="MKKVLLLIAACVFSLLSVTARADAIFAPDASGITGSTVSLNVSL
                     LSGDISFGGDFTIGYDTAKLTLLDVLAGDPMFSPLRSFTPGSESVSLVNSSGSSYGSG
                     PLFSVLFRILATFPDSTPVDISGVLYDAAGGTNALTTISPLVTVDQRTGTLPEPGVFG
                     LAGAALLAMAVRRMRRG"
     gene            complement(45242..45317)
                     /locus_tag="PKX01_01210"
     tRNA            complement(45242..45317)
                     /locus_tag="PKX01_01210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45282..45284),aa:Gly,seq:gcc)
     gene            complement(45330..45403)
                     /locus_tag="PKX01_01215"
     tRNA            complement(45330..45403)
                     /locus_tag="PKX01_01215"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45369..45371),aa:Cys,seq:gca)
     gene            complement(45444..45519)
                     /locus_tag="PKX01_01220"
     tRNA            complement(45444..45519)
                     /locus_tag="PKX01_01220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45484..45486),aa:Gly,seq:gcc)
     gene            complement(45579..46151)
                     /gene="pgsA"
                     /locus_tag="PKX01_01225"
     CDS             complement(45579..46151)
                     /gene="pgsA"
                     /locus_tag="PKX01_01225"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HMZ74766.1"
                     /translation="MPLNIPNSLTWARIALIPFFVGIYYLPADVIGPDDKNLVATLTF
                     VAAAVTDWFDGYLARSLGQTSAFGAFLDPVADKLMVAAALIVLVHLGRADSLIALIII
                     GREITISALREWMAKVGASASVAVAFVGKLKTAAQMSAIPLLLYDDQLGGFDTRPFGT
                     LLIYVAAVLTLWSMGYYMRCAWPELMKRSG"
     gene            complement(46167..47963)
                     /gene="uvrC"
                     /locus_tag="PKX01_01230"
     CDS             complement(46167..47963)
                     /gene="uvrC"
                     /locus_tag="PKX01_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMZ74767.1"
                     /translation="MNFDAKSFLRTLTEEPGVYRMIGADESVLYVGKAKNLKRRVASY
                     FQRTQLSPRIALMVAQVARVDVTPVRSEAEALILENNLIKSLGPKYNILFRDDKSYPY
                     IALSGGEYPQIYYHRGGFQKGVRYFGPFPNSWAVRESLHLMQKIFRLRTCEDNTFSNR
                     SRPCLLYQIQRCTAPCVGKIDAAAYAADVQLASLFLNGRHGDVIDDLSRRMEAASERL
                     AFEQAMVYRDQIRNLQAVLHKQFVESSKDEDVDVLAAVEQGGLVCVNLAMVRGGRHLG
                     DRPLFPANAQGCAPLDALAAFVEQHYLEHPLPGKILVNGDVATLREALEAVVEGKAPV
                     AAPRFENERAWMGMAENNAGLALLARQQVSARGEQRLDALRDALALGDPPKRIECFDI
                     SHTLGEATVASCVVWDGAGMKKSEYRRYNIAGITPGDDYAAMRQALTRRYEKVAAGEG
                     ARPDLILIDGGKGQLGVAHEVLTELGLTEIGLVGVAKGESRKPGLEELVFPDGRAPVH
                     LPPDAPALHLIQEVRDEAHRFAVAGHRARRAKTRNTSRLEDIAGVGPTRRRKLLAAFG
                     GIDGVRHATIEDLCRVDGINRQLAEQIYNALR"
     gene            complement(47982..49019)
                     /gene="nagZ"
                     /locus_tag="PKX01_01235"
     CDS             complement(47982..49019)
                     /gene="nagZ"
                     /locus_tag="PKX01_01235"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HMZ74768.1"
                     /translation="MKLSHRPPGPVMLDVAGLELAAEERDILQHPLVGGVILFARNYA
                     DSAQLRALTAAIRALRPGLVISVDHEGGRVQRFRDGFTRLPAMRRFGALWDADPEHAR
                     GAARQAGLVLAAELRAHDVDLSYAPVLDLDYGVSGVIGDRAFHRDPQVAAELARALAQ
                     GLAAAGLKAVGKHFPGHGAVEADSHVAIPVDHRSFDAVWAEDIVPFRVLARELGGVMP
                     AHVIFEGIDPRPAGFSPFWLQDVLRGRLGFTGVIFSDDLTMEGASVAGDIVARAAAAQ
                     RAGCDMVLVCNRPDLAVQLLDAWQPQPTADSLARIAALRPAPGGVPGDPLTLARWAPY
                     CEARDAVLALA"
     gene            complement(49016..49393)
                     /gene="acpS"
                     /locus_tag="PKX01_01240"
     CDS             complement(49016..49393)
                     /gene="acpS"
                     /locus_tag="PKX01_01240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HMZ74769.1"
                     /translation="MIHGIGTDIVRVARLDEALARHGARFAERILAEPERVAWQAARQ
                     PARHLAKRFAAKEAFGKALGTGVAAPATLHAVRVGHDPLGKPVFEYAPALAAYLAERG
                     LVAHLSLSDEADYVLAFAVIEQP"
     gene            complement(49390..50148)
                     /locus_tag="PKX01_01245"
     CDS             complement(49390..50148)
                     /locus_tag="PKX01_01245"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMZ74770.1"
                     /translation="MIELGVNIDHVATLRQARRTWEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDADVRKLRELTQIKLNLEMAATDEMVGIACALKPELAMFVPEGRHEVTTE
                     GGLDIVSQEARLKDAVARLADQGIITSVFIDAEVAQVEAAARIGARVCEIHTGPYAHV
                     FHDAGRDAESPAVVAEIDKIARAGEAIRALGLRFNAGHALNYYNVQPIARLPGVRELH
                     IGHSIVSRSVFTGLREAVREMKRLMREAAERGAR"
     gene            complement(50145..50882)
                     /gene="recO"
                     /locus_tag="PKX01_01250"
     CDS             complement(50145..50882)
                     /gene="recO"
                     /locus_tag="PKX01_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMZ74771.1"
                     /translation="MAAKQRVDQQPGFVLHSYPYRETSLIVEVFSRDHGRVGLVAKGA
                     RRPSSALRGTLMAFQPLLIDWSGGGEVKTLIRAEWQGGQPLLGGQALLVAYYANELLM
                     RLLPREDPHPELHHAYGEALRTLAGGEAQEVVLRRFELALLQELGYGLPLEEDVDGQP
                     VRPDRFYAYIIERGPVPVDDAGIEEAALVSGRTLLHMARGDFSDPDILAGSKALMRRL
                     IQHYLGGQVLQSRRVFTELIAFPGEPS"
     gene            complement(50916..51824)
                     /gene="era"
                     /locus_tag="PKX01_01255"
     CDS             complement(50916..51824)
                     /gene="era"
                     /locus_tag="PKX01_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMZ74772.1"
                     /translation="MTEHTVSADTPFRTGFVAIVGRPNVGKSTLLNRLIGQKISIVSR
                     KAQTTRHRVTGVLTEGGAQFIFVDTPGFQTRHKNALNRAMNRTVTQVLADVDLVLFVI
                     ESGRFGPEDEQVVKLLPEGARVILVINKVDMLADKARLLPFMERMSGVYPFTEIVPVS
                     AEKGQNTAQLLKAAMPYLPEGEPIFGEDDITDRSERFLAAEFLREKLFRQLGEELPYG
                     MTVEIEKFEAEPGLRRIHAAIIVDKAPHKAMVIGRGGERLKRIASEARVDMEQLFDSR
                     VFLEVWVKVKGGWADDERALKSLGYE"
     gene            complement(51821..52492)
                     /gene="rnc"
                     /locus_tag="PKX01_01260"
     CDS             complement(51821..52492)
                     /gene="rnc"
                     /locus_tag="PKX01_01260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMZ74773.1"
                     /translation="MKLDALQVALGHRFNRPELLVQALTHRSFGSPHNERLEFLGDSV
                     LNCVTAIALYDRFAALREGDLSRLRANLVRQEALFRVAETLRLGECLKLGEGELKSGG
                     HRRPSILADALEAVFAAVFLDAGFETAKAVIDRLYADALVAIDPASALKDPKTALQEW
                     LQGRRMPLPRYSLADTRGEAHRQEFEVECEIAGLGLKTRGVGVSRRAAEQQSAQRALE
                     RLPKS"
     gene            complement(52501..52848)
                     /locus_tag="PKX01_01265"
     CDS             complement(52501..52848)
                     /locus_tag="PKX01_01265"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HMZ74774.1"
                     /translation="MSIIGMALSAVLMGAALLVFLKVVPAVSEYMGVRRAISAVASGA
                     DPTTATVSQLRDAFTKRARIDDITSIGASELDITKEDGKIVITASYSRKIPLVSNVSL
                     LIDFYATSAPTGR"
     gene            complement(52875..53711)
                     /gene="lepB"
                     /locus_tag="PKX01_01270"
     CDS             complement(52875..53711)
                     /gene="lepB"
                     /locus_tag="PKX01_01270"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ74775.1"
                     /translation="MNFALILFVLLLISGALWALDHFVARKQRGAGAPPPWWVEYGAS
                     FFPVILVVFCLRSFVVEPFKIPSGSMLPTLLVGDFILVNKWTYGIRLPVINKKIVDIN
                     QPRRGDVMVFRFPADPSLDYIKRVVGLPGDKVEYLDKKLVINGVEMPQRPDGDFLHPD
                     ALDPSRQGDGKGADRQTRMFSPRFIEKLGDVEHSILIEPDVPSFVSQVVQFPQRENCT
                     YTSRGVSCTVPEGHYFMMGDNRDGSFDSRGWGFVPDANIVGKAFFIWFNFNDLKRIGS
                     FH"
     gene            complement(53758..55554)
                     /gene="lepA"
                     /locus_tag="PKX01_01275"
     CDS             complement(53758..55554)
                     /gene="lepA"
                     /locus_tag="PKX01_01275"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMZ74776.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRIIQRCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALSYRARDGQVYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTALDQGVEVVPVLNKMDLPQANPDGAKQEIEDVIGIDATDAIP
                     CSAKTGLGVEDILEAIIARVPPPKGDPEAPLKALIIDSWFDNYVGVVMLVRVVEGGLR
                     PKDKIQFMATGVQQLCEQVGVFTPKSVARETLSAGEVGFVIAGIKELKVAKVGDTITL
                     ANKPAAEPLAGFKEIKPQVFAGLYPVESNEYDQLRESLEKLRLNDASLQFEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVLNDGSVEQVSNPSRLPE
                     ASKMAEIREPIITSTIFLPQDYVGPVITLCNQKRGMQVDMRYHGRQVQLIYDMPMNEV
                     VMDFFDKLKSVSRGYASLDYEFKEYRAADLVKLDVLVSSEKVDALSVIVHRSTSQYRG
                     RELVAKLRELIPRQMFDVAVQAAIGSHIIARETIKALRKNVLAKCYGGDITRKKKLLE
                     KQKEGKKRMKQVGNVEIPQEAFLAVLRVDEGK"
     gene            complement(55621..55872)
                     /locus_tag="PKX01_01280"
     CDS             complement(55621..55872)
                     /locus_tag="PKX01_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HMZ74777.1"
                     /translation="MSADRHLTVLSRAWCHLCHDLLDALAPLQVELGFSVEVIDVDSD
                     PELEERWNEAVPVVLAGEGELCRHFLDAAAIRAHFRRIG"
     gene            complement(55862..57286)
                     /locus_tag="PKX01_01285"
     CDS             complement(55862..57286)
                     /locus_tag="PKX01_01285"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HMZ74778.1"
                     /translation="MQRTLGRFIWVMVLLGALMLVRPAWARDLPDVAELAERQGAAVV
                     NISTTQAGRSGVRGAHPNLDEDDPMFDFFRRFVPRGPGGVPRMDPDDRSLGSGFIVSS
                     DGYVLTNAHVVDSADEIVVKLTDKREFRARLIGADARSDIALLKIEASGLPRVAFGDP
                     ARLRVGDWVVAIGAPFGFENSVTAGIVSAKGRALPQENFVPFIQTDVAINPGNSGGPL
                     FNMKGEVVGINSQIYSRTGGFMGLSFAIPIDVAMDVQAQLRQNGRVQRGRIGVVIQEV
                     SRDLGESFNLARPAGALVSSVEKGSPADKAGFEQGDIVLRFDGRSVGLSSELPRLVGG
                     TRPGSRVVAQVWRRGVQKDLTVTVAELPDEKPQKVIAKVMRPEAPPNKLGILVVDPNA
                     EQRRKIGAGVVVDALRSPSARASEIQAGDAILALVSRGTRVDIRSADQFNRVVAAFEG
                     GQAVSALVQRGDVQTFVPLRTLER"
     gene            complement(57286..57729)
                     /locus_tag="PKX01_01290"
     CDS             complement(57286..57729)
                     /locus_tag="PKX01_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HMZ74779.1"
                     /translation="MRVPGVVQKVEQGFAWVEVTPAGGCGRCHEPGGCGGVSITRALE
                     TSRKTYRLPNVPGCVPGERVGVVIEDGVALRAALLTYGLPVLGVIAGAALGTVLATPE
                     RGDLGAVVGAVLGLAGGFVAGRGRWGERTSPVRLVRDADGTGCGA"
     gene            complement(57729..58697)
                     /locus_tag="PKX01_01295"
     CDS             complement(57729..58697)
                     /locus_tag="PKX01_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HMZ74780.1"
                     /translation="MSRFRHCLLVLLACCGTARAEAPGDPLSWLGRIAGAAHRLNYSG
                     TFTYQHGKTVETSRIAHLVDGSGEFERLEALDGSPREVVRSNDQVQCYLPDQRMLIVD
                     QPSVRRSFPARLPPSVNTLAENYRIRKGEMVRIAGLESQQIILEPKDNLRYGHQFWAD
                     VNSGLLLRARLIDEKGDSIEQFAFSEVQIGVPLDRDKVRAHIVRDPDWKVVNARGTEV
                     RMEDAGWVFRNALPGYRQVVSVLRQIRQDRPEAYHVVFSDGLSAISVFIEPHAGKSSV
                     IQSGMSATGSFNILRRSAGDYLITVLGEVPAAALVRLADGIELRKK"
     gene            complement(58694..59239)
                     /locus_tag="PKX01_01300"
     CDS             complement(58694..59239)
                     /locus_tag="PKX01_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HMZ74781.1"
                     /translation="MKERVSALLDGALDEESTVRMLDHLKRDGALRRDWEHFCLVGDA
                     LRGDEPLALDVTRSVMLRLEDEPTVLAPAVRREGSGFFRHLMPLAASLMGVAAVGWVA
                     GMFSGGGDALHFAKVNKSPALMASVTPATSGQSMVMPTSVENSNREYLFAHQSLAPSA
                     AMPGVALYVRTVADTAMPGQR"
     gene            complement(59244..59843)
                     /gene="rpoE"
                     /locus_tag="PKX01_01305"
     CDS             complement(59244..59843)
                     /gene="rpoE"
                     /locus_tag="PKX01_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HMZ74782.1"
                     /translation="MSDREIDQQLVERVQRGDKQAFGLLVAKYQRKLARLLSRLIRDP
                     AEVEDVAQETFIKAYRALPSFRGESAFYTWLYRIGINTAKNFLVSQGRRAPTTTEFDS
                     EEAETFEDGDQLRDVDTPERLLMTKQIGETVNLAMEALPEELRAAIVLREIEGLSYEE
                     IATIMGCPIGTVRSRIFRAREAISEKLKPLLDTAPNKRW"
     gene            60046..61629
                     /gene="nadB"
                     /locus_tag="PKX01_01310"
     CDS             60046..61629
                     /gene="nadB"
                     /locus_tag="PKX01_01310"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMZ74783.1"
                     /translation="MSVFDVLILGSGLAGQSLALRLADTHKVALVTKRHLEDSASAWA
                     QGGIAAVLDPADSIESHLTDTFVAGAGLCNPGSSRFVVENGARAIQWLIERGVPFTAD
                     AQSPIGYHLTREGGHGQRRVIHVADATGAAVQATLTEQVRAHPNIAIFEQHITVDLIV
                     GSKIGRLEEGCLGAYVLDIAHDEVRTFAAGHTVLATGGAGKVYLYTTNPDTATGDGIA
                     MAWRAGCRVANMEFIQFHPTCLYHPQAKSFLISEAVRGEGGLLRRPDGSRFMPEHDTR
                     EELAPRDVVARAIDFEMKKHGLDCVFLDISHKPAEFIAEHFPNIHARCLELGIDITQQ
                     PIPVVPAAHYTCGGVVTDLHARTDVPGLYAIGETACTGLHGANRLASNSLLECLVFAE
                     AAAQDIRSHLRHAPADLPTWDESRVTDADEAIVISHNWDELRRFMWDYVGIVRTTKRL
                     QRAQHRIRLLAREIDEFYSNFRVTNDLIELRNLVVTADLIVRSALRRKESRGLHFSRD
                     YPQLQPKARETVLRPPRRS"
     gene            complement(61652..62086)
                     /locus_tag="PKX01_01315"
     CDS             complement(61652..62086)
                     /locus_tag="PKX01_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HMZ74784.1"
                     /translation="MINVLEFGPSRLRVILLVTLHAAAAFSCVAGLASPWITAPVGLV
                     IAASLYREVKQALGQSGRLELHADGRLAWQFSGMAAQAGRVRAESVALHRSIWLIWDD
                     DGRRGARWLLPDQMSTQDWRALQVWLRLRVTKTFAEPDSDVA"
     gene            complement(62117..63352)
                     /gene="fabF"
                     /locus_tag="PKX01_01320"
     CDS             complement(62117..63352)
                     /gene="fabF"
                     /locus_tag="PKX01_01320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMZ74785.1"
                     /translation="MAARRVVITGLGVISPVGNTVSEAWDSILNGRSGIGPVTRFDAS
                     ALSARIAGEVKGFDVGAYISAKEARRMDTFIHFGLAAGIQAIRDSGIEVTDANADRIG
                     VNIGSGIGGLPMIEGTHTDYVAGGPRKISPFFIPGTIINMISGNLSIMFGLKGPNLAV
                     VTACTTGLHAIGSSMRMIQYGDADVMVCGGAESTVCPLAMGGFASAKALSTRNDDPAT
                     ASRPWDKDRDGFVLGEGAGVMVLEEYEHAVKRGARIYAELAGFGMSGDAFHMTAPDTD
                     GPRRSMLNALRDAGVNPDQVQYLNAHGTSTPLGDKNETEAIKQAFGVEVAKTLVVNST
                     KSMTGHLLGGAGGLESVLTALAVHHQVSPPTINIFEQDPECDLDYCANVARPMKIDVA
                     LKNNFGFGGTNGSLVFRRV"
     gene            complement(63375..63614)
                     /gene="acpP"
                     /locus_tag="PKX01_01325"
     CDS             complement(63375..63614)
                     /gene="acpP"
                     /locus_tag="PKX01_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ74786.1"
                     /translation="MSDIEQRVKKIVAEQLGVNEAEVKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITTVQQAIDYVSAHLNK"
     gene            complement(63702..64451)
                     /gene="fabG"
                     /locus_tag="PKX01_01330"
     CDS             complement(63702..64451)
                     /gene="fabG"
                     /locus_tag="PKX01_01330"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMZ74787.1"
                     /translation="MSQVLQGQVALVTGASRGIGRAIALELGRLGATVIGTATSEAGA
                     ADIQAGLDAAGVSGRGMMLNVTDAAACEAALAEIEKAFGPVLVLVNNAGITRDNLSMR
                     MKDTEWDAVIDTNLKAVFRMSRLVTKGMMKARNGRIINITSVVGSSGNPGQANYAAAK
                     AGVAGMTRSLAQELGSRNITVNCVAPGFIDTDMTKELPEDQKKALQTKIALGRLGKPE
                     EIAAVVGFLASPAAAYVTGATLHVNGGMYMA"
     gene            complement(64448..65383)
                     /gene="fabD"
                     /locus_tag="PKX01_01335"
     CDS             complement(64448..65383)
                     /gene="fabD"
                     /locus_tag="PKX01_01335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMZ74788.1"
                     /translation="MNFAFVFPGQGSQSVGMMAAYGDSAVIRDTFAEASAALGEDLWQ
                     MVADGPAETLALTVNTQPLMLTAGIAVYREWIARGGRTPALVAGHSLGEYSALVAAGA
                     LGFQDAVKLVRLRAQAMQEAVPAGEGAMAALMGLDQPAAAEACAEAAQGEVVSPANLN
                     SPGQIVIAGARAAVERAIAVAKTKGAKRGVLLPVSAPFHCALMKPAAERLAVALADIV
                     VAAPAIPVLNNVDVAVETDPARIKDALVRQAYSPVRWIESVQAIAGRGLTHVYECGPG
                     KVLAGMTKRIEGSLTGGAIADAASLDEILQTTGAA"
     gene            complement(65416..66381)
                     /locus_tag="PKX01_01340"
     CDS             complement(65416..66381)
                     /locus_tag="PKX01_01340"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ74789.1"
                     /translation="MIYARVAGTGSYLPGAPVTNDDLVARGIDTSDAWIQERTGIRSR
                     HLASPEVTSSQLAQEASLRALEAAACTPNDIDLVIVATSTPDYIFPSTATLLQARLGM
                     SNGAPAFDVQAVCAGFAFAIATAEKFIRSGSHKRALVVGAEVFSRILDWNDRGTCVLF
                     GDGAGAVVLEASERPGILATALHADGAQNQILCVPGQISRGQVTGDPFLRMDGQAVFK
                     FAVKVLSDVAKEVAELGGVPLDAVDWLIPHQANIRIIQATGKRLGVAPERVIATVGEH
                     GNTSAASIPLALDAAVRDGRIRRGQKVILEGIGGGFAWGALLLEY"
     gene            complement(66378..67391)
                     /gene="plsX"
                     /locus_tag="PKX01_01345"
     CDS             complement(66378..67391)
                     /gene="plsX"
                     /locus_tag="PKX01_01345"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HMZ74790.1"
                     /translation="MTVTLAIDCMGGDHGPSVTVPAALRFLDTDPDVRVVLVGRAEAV
                     EQQLGVAQVRYGERLSIRHATEVVGMDEPPALAMRNKKDSSMRVAVDLVKSGDAQAAV
                     SAGNTGALMAISRFVLKTLPGIDRPAICSTLPTLRGQTHVLDLGANVDCSPAHLLQFG
                     IMGAMLVAAVEHNERPSVGLLNIGEEDIKGNETVKQAGELLRHSGLNFYGNVEGNDIY
                     KGTVDVVVCDGFVGNVALKTSEGLAQMLATFLREQFSRNWVTKLAAVFAMSALKGFKH
                     KVDSRRYNGAALLGLRGVVVKSHGAADVFAFEQAIRRAVEAARNNLIERISARMERER
                     PAA"
     gene            complement(67470..67649)
                     /gene="rpmF"
                     /locus_tag="PKX01_01350"
     CDS             complement(67470..67649)
                     /gene="rpmF"
                     /locus_tag="PKX01_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMZ74791.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLVAPPLAVEPTTGEVHLRHHVSP
                     SGVYRGKKVAKAKGE"
     gene            complement(67671..68165)
                     /locus_tag="PKX01_01355"
     CDS             complement(67671..68165)
                     /locus_tag="PKX01_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HMZ74792.1"
                     /translation="MSQEGFVIDLRALARDGRHVEGEIPVARLERLADSVSDPVGSVW
                     FRFDGVRDDDGKLFADLMIRTELTLQCQRCLDALRLPCVIHNRLLLLRPGEEIPEDDL
                     ENDEVDALEVEPRTDVLALVEDELLLALPLVPRHEACEAPVKAGGDEEISPFAVLRNL
                     RGKA"
     gene            68265..68840
                     /locus_tag="PKX01_01360"
     CDS             68265..68840
                     /locus_tag="PKX01_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HMZ74793.1"
                     /translation="MKLVLASTSAYRRELLLRFGLPFDVARPDIDESPLPDESPRATA
                     ERLAVGKARAVAAQFPDALIIGSDQVAALGEHRFGKPGCVERAVAQLKTMSGQTVVFH
                     TALALLNTRNGAVQVDVVPTEVRFRALSEDEIVRYVERERPLDCAGSAKSEGLGITLL
                     DALTGDDPNALVGLPLIALARMLRAEGIALP"
     gene            68840..69550
                     /locus_tag="PKX01_01365"
     CDS             68840..69550
                     /locus_tag="PKX01_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ74794.1"
                     /translation="MAGTLYLVPVSLGPAPTALTTPAGVAAQACALDYFVVENAKSAR
                     AELKRLEHPRPLRELDIRELPDKPNPADLDALLAPILAGTNGGLMSEAGCPAVADPGA
                     LLVRRAHEAGVRVVPLVGPSSLLLALMASGLNGQSFAFHGYLPVDEDARNRRIRELEA
                     ESGRQQRTQLFIETPYRNERMFEALRTVCAPTTRLCVARDLSLDSEWIATRTVAAWRK
                     SAAPELARHPTVFLILAG"
     gene            complement(69555..69893)
                     /locus_tag="PKX01_01370"
     CDS             complement(69555..69893)
                     /locus_tag="PKX01_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMZ74795.1"
                     /translation="MICRSDEVLEGGDGIRFTVQVCGEAVAAFLVRYGGRVYAYRNSC
                     AHVPVELDWQPGRFFDTGGTYLICATHGALYEPESGYCVAGPCRGRRLVPVAVEERDG
                     GVFLKGVLGA"
     gene            complement(69890..70546)
                     /locus_tag="PKX01_01375"
     CDS             complement(69890..70546)
                     /locus_tag="PKX01_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMZ74796.1"
                     /translation="MVKRFDLIVFDWDGTLMDSAAAIVRAIQSASTDLGLPVPDDARA
                     RHVIGLGLVDALRHAVPDLAEHDYPRMVERYRHHYLSGDHELTLFDGTFDMVEALHGR
                     GHLLAVATGKSRKGLERALGFSGLGRFFHATRCADECFSKPHPQMLEELLDELGVMPD
                     ACLMIGDTTHDLQMARNAGVAALAVSFGAHPVEQLEAEAPLACLHSPVALRDWLERHA
                     "
     gene            complement(70539..71459)
                     /locus_tag="PKX01_01380"
     CDS             complement(70539..71459)
                     /locus_tag="PKX01_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ74797.1"
                     /translation="MVDESAAGQRIDNFLIRHCKGVPKSHVYRILRSGEVRVNSRRVA
                     QTYRLQSGDEVRIPPLRLAEREDSSPVPVGKAFAIAYEDEALLVLDKPAGLAVHGGSG
                     VSFGVIEQLRRQRPEARFLELVHRLDRETSGLLIVAKKRSALTVLHDMMRDGRVQKRY
                     LTLVAGRWMNPLQHVKAPLLKYLTEGGERRVSVHPDGKPAHSIVRLHRRWQSCSLVEV
                     ELKTGRTHQIRVHLTHQGFPLYGDDKYGDFALNKQLEKAGLKRMFLHAAHLAFRHPLT
                     DAPIELSAPLPDELAAFIVAVEQAEERDYG"
     gene            71947..>73117
                     /locus_tag="PKX01_01385"
     CDS             71947..>73117
                     /locus_tag="PKX01_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMZ74798.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKA
                     VITRIEPSLEAAFVDYGAERHGFLPFKEISRAYFQPGVEASRASIKEALKEGQELIVQ
                     VEKDERGNKGAALTTFVSLAGRYLVLMPNNPRGGGVSRRVEGDERAELRDVMDQLEVP
                     GGMSLIARTAAIGRSAEELQWDLNYLLQLWRAIDGAAQAQAGAFLIYQEGSLVIRAIR
                     DYFQPDIGEILIDTDDVYEQARQFMAHVMPTNVNRVKRYHDDVPLFSRFQIEHQIESA
                     YSRTVNLPSGGAIVIDHTEALVSVDVNSGRATRGADIEETAFKTNCEAADEVARQLRL
                     RDLGGLIVIDFIDMESQKNQREVENRLRDALRYDRARVQTGKISRFGLLELSRQRL"
BASE COUNT        12347 a        23819 c        24625 g        12326 t
ORIGIN      
        1 ccactgccgc cccaggtgcc ctccgccccc tccaccgacc tgacggttcg cccgcctgca
       61 tcccctgcca tagcaacgcc agccggcccc cgcgtcacgc tccgggacat caccctgtcc
      121 ggcaacaccc tcctcgacag caccacgctg caggccgggc tgggcccggt cagcgggcag
      181 tccttcgatc tggccgggct cgaagccctg gctgcgggaa tcgagcgcca gtatcgcgct
      241 gccggttatc ccttcaccca ggccgtgctg ccgccccagg acctgaagga cggcacgctg
      301 cgtatccaag tggtggaagg ccgttacggc agaatccagg ccacgggcac cgacccgctg
      361 cctgcctccg cccagccgtt cctggatttc gggctgcggt cgggcgatgc catcgaaaac
      421 gcccgcctgg aacgcaccct gctgatcctc gacgatcagc cgggaatgaa gatccgtccg
      481 gtgatccagc cgggcgaccg gaccggcgag gccgacttgt cagtcaacgt ggagcgggtc
      541 tcccttgtgg gcggcgaggt cggcctggac aacactggcg cccgctccac cggccagaac
      601 cgggctcgcg gcgccgtgtt catcaacagt cccttccgtt acggcgacaa gatttccatc
      661 aacgccctgg caaccgacca gagcatgctg ctcgggtccc tcgactacga gatgcccatt
      721 ggcgcatccg ggctgcgcgg acaggtgggc tacgcctaca ccaactacca gctcggtgcc
      781 cagttcgccg ccctggatgc caagggctac gccaagatca ccacggcccg cctgagctat
      841 ccgctgatcc gctcccaggc aagcaatgtg ctgctgtccg caggctatca gcacaaaaac
      901 ctggaggatc ggtacgacag caccggcctg atccgcaaca agcacagcga cggcacgccg
      961 gtcgcgatcc agttcgaccg gcgtgacacc ctgctcggtg gcggcgtgac gtatggctcg
     1021 ctgacctggc tgcccggctc cctgagcctc gatccgggca tggatgccct ggaccgggtc
     1081 accgcccgga cggccggccg cttcagcaag ctgaatctcg atgtcgcgcg catccagcag
     1141 ctcacctcca gcgtccacgc ctacgcgcgc tattccggtc aatgggcgga caagaacctg
     1201 gattcgtcgg aaaaattcaa cctgggcggc ttctatgggg tgcgcgcctt cccgctgggc
     1261 gaaggcgtgg gcgatcaggg ctggttcaca cagctggaaa tccgctacga cgtgggcgtc
     1321 gtgaccccct tcgccttcta cgatgcgggc cacgtggaaa ccaacgccaa gccctgggac
     1381 gtcaattcgg cctccacgcg ccggctggcc ggctccggtg tgggtgtgcg ttccctcagg
     1441 gacggctgga gcctggacgc caccctggcc tggcgcactc agggcggcgc ccccaccagc
     1501 gaggacgtgg atcgcaatcc ccgcctcttc gtgatgatcg gccggcgctt ctgaagtcat
     1561 cccgctgaca aagccgctgc ggcgcggggc ccgcgggctc agtggctggc cggggcgccc
     1621 ggcgcagtcg gtgccgccgc cggcttcttc ggcggcgcca gcaccgtacg tcctgtcacc
     1681 cccggcacgc tggccttgca gcggatgccg gcgggcagct tgaggccggg gttggggagt
     1741 tccatgcgca cgccgaaggt gccgctggcg gcgtccatga cccggtcgat gaccttgacc
     1801 gtcgcagtgt agcggccgcc gacggggctc tccggcagga tctcgatgct ctggccgacg
     1861 gccagcttgc cccacgcgtc caccggcagc agggcctcca cgtgcagggt gccgatgtcg
     1921 gcgagcttga ggatgggctt gcgcaggtcg cggttgtcgg cgagctcgcc ggcgtgcagc
     1981 aggcgatcca ccaccacgcc gtccaccggg ctcttgatgg tgcgcaggcg cagttgctcg
     2041 gacaggcggc ggtattcgat ctcggccagc ttgcggtcgt cgcgggcctc gatcagctcg
     2101 gattcggcga ggcgcttctc ggtgagggat tcgtcccggt cctgcatgga gacaaaggac
     2161 tggcctgcca gcttgtcgcg gcggttgtac ttctgggtgg cgaactcgac gcggctctcg
     2221 ccggtgcgga tcttgccttc catcgtggcg cggtagcggg cggattcggt ggcggctttc
     2281 tcgacgcccg aatccagggt gaccagcacc tggccggcct tgaccatgtc gccccgatcc
     2341 acccagatct tttcgatgag cccttcgctg gcggcgcgca cgtccaccgt cttgcgcggc
     2401 tcgatcatgc agtcgaactc gcccgcgcgg gccgcggaga cggcacccaa ggtgttcagg
     2461 acggccagga cgaccccggc ggtggtgcgg cgtttcatgc tcattcccct cgcccggcaa
     2521 atgcgagcgc ttgttccatg cggttgtcgg aatccaccgt gtgacggcgg atgcgggcgt
     2581 tctgggcttc ggcggcgccc tgggcgaagc ggcgcaggag cggcccgaag cggcgcgcca
     2641 cctccccgtc cggcgccgtg cggacgcgca gccaggccac caggcgggca gcgtgggcaa
     2701 cgaagagctc gtcctccagg ctcaccaggg cctcgtagga gccggggtcg ccctggcgcc
     2761 cggagcggcc gtagagctgc cggtcgatgc gcgccgagtc gtggtattcg gtgaggatca
     2821 cgtgcagccc gccggccgcc ttgacctcgg gcgtcagcag gatgtcggtg ccacgcccgg
     2881 ccatgttggt ggccacggtg atgcggccgg gctggccggc gtcggcgacg atctccgcct
     2941 cgcgggcatc ctggcgggcg ttgagcactt cgtgatccag accggccgca gccagacgcg
     3001 caccgatgag ctcagacgcg gccaccgagc gggtgccaat cagcacggcg cggccagcgg
     3061 cggcgtgggc gcgcgcggaa gccagcaccg catcccagcg cgcctcgctg cgggcgaaca
     3121 cgcgcaggcc caggtcacgg cgctgcactg gccggttggg cgggatgcgc agcacgtcca
     3181 gggcgtacac ggtgcgcatc tccggcgcca cctcggtgac ggtgccgctc atgccggcca
     3241 ggcgcaggta gcggcggaag aaacgctggt aggtgatgcg cgagatggtg tcgcggcgct
     3301 tggtgagctc caggccctcc ttcacctcga tcatctggtg cagacctttt tcccaggagc
     3361 ggtcggccat gacgcggccg gtgtactcgt ccacgatctg caccttgccc tcggccagga
     3421 tgtagtggcg atccagttca aagaggtgtt cggcggtgag gccctgctcc accagttcct
     3481 cccgcgcgcg cgacacctgc cacaggccgg gcaggccgcg ggacgcctcg gccacggcgg
     3541 cgcggccggc ttcggtgagt tcgatgctgc gttccttgag gtgcaggcgg tagtgctcgt
     3601 cggcgttgag gctgcgggcc acggccaggc cctgctcgta gaaggcgcga ccgttggggt
     3661 cgtcctgctc ggcggaaatg atcagcggcg tgcgcgcctc gtcgatgagc acgctgtcag
     3721 cctcgtccac gatggcgtag tacagcccgc gcagcagggt ccccgagccc cccaccggcg
     3781 cgctgcggcc gagccagcct tccagcagcg cccgcgcacc cgccttgatc cgctgggcgg
     3841 tgatgcggtc gcgcaggtag tcgaagacca gatccttgtt cacgcagtag cacacgtcgc
     3901 agccgtaggc gacgatgcgc tcgggcggct cctgctccgg cgtgaccagc ccgacgctga
     3961 ggccgaagaa gccatacacc ggcgcgagct gctcggaatc ccgctgggcg aggtattcat
     4021 tgacggtgat cacatgcacc ggcacgccgg ccagggccac cgccaccgcg ggcagcaccg
     4081 cggtgagggt tttgccctcg ccggtcatca tctcggccag gccgccattg agcatggtgt
     4141 agccgcccat cagctgcacc gggtagtggc gcttgcccag gcgacggaaa cacacctcgc
     4201 gcacgagggc aaagacctcc gccgcgttgg cgtcgtcgag cccgctggcc gcgagagcgg
     4261 cgcgcagggg cgcgacgcgg gcttgcaggg tggcttcgtc cagggcagcg agttcgcctt
     4321 cgtgggcgat cacctgttcg gcaaaacggc gcaccggcgg caggcgcaag gcagaaccgt
     4381 ggcgcgccca gcggccgagc agaccgccca tgaagcggtc cagcttgcca ggcttggcat
     4441 cctgccgttc gggatacagg ccgggggcga aggcatggac gccccaggtg gacagcacgg
     4501 ccctagacat ggaagcggga caggaagagt tgccgcaggc gccggcccca ctgatcggcc
     4561 aggggctcgg cccggtggat gaagcgcacg tagacgcggg tgccaagctg cacctcgcca
     4621 acgctgtcgg gcagggccag atcgaactgg aagagccgct ccagggtctt gaggccatcc
     4681 ggatcgcggg gatcggtggc gtgcaggccg ccgccttcca tggccagggc gcgattgggg
     4741 agctgatcct gagcgccggg cacctcacga ctcacctggg ccggataggt ctcggcaaga
     4801 cggtcggcga tcttcacctc caccgcctcc aggcgatccc gcaccagggc gatgtcgtcc
     4861 tgggtgacca ccgcgcgtac cacccgcggc gggcccgcca gcaggtagcc gaccagctcg
     4921 cccttcttga cgaagtgctc gggcagatcg tccgggcggg acaggcgcag gcggccggac
     4981 acgccggcgg tgagcagcag ctcgtccagg cgctcctcgg cgcgggccag ggcggcgcgc
     5041 tcgcggccca gatcctcggc caccacggcg gcctgcaggc ggtcgtcgaa catctgggcg
     5101 gcgtactgca cctctagctg gcgcacccgt tcgcggcgct gggtcacatc ggcatccaca
     5161 tccggctggc gggtgacgat gaccagatcg ccggcctgca cccaggcgtc cggcgcggcc
     5221 agcacgcgct ccacgaatcc attccccttg gcccgcagct cggcctgggc cggtacccag
     5281 acgatgccct cggcgtgggt gcgcaggggc atcggcacaa cgaagagaaa gagcagcagc
     5341 gccgccagcc cgccaaaggt gacccacagg gcacgcggcc ggtggcgctg gagctcggca
     5401 ctgccccaca catagcccag catcttgccc accggcacgc cgaacatggt gatggcgccc
     5461 cagatcgcga tcaccacgcc gatgaagaag aactcgccgg cgatgaaaag ggtgatggag
     5521 atcgtcacga acagccggta cagggccgag gccgggccgt agaacaggaa ccagcgccgc
     5581 tcgcccggcg tggccgcggg gcgctccacc tccaccgcgc cgaacaggtg gcgcttggcc
     5641 agccactgcc agtactggtt ggcgcgctgg gccaggttgg ggatctcgat gaggtccgcc
     5701 aggatgtaat agccgtcgta gcgcagcagc gggttgccgt tgaacaccag ggtggacagc
     5761 ccggccacga agatcacgtt gtaggccacc gagcgcacga agccgggctc cgcgaggacc
     5821 cacagcatca ccgccagggc ggccagaaag agctccacca gcatgcccgc cgcgcccacc
     5881 agggcgcgcc gccacttgct gcggaaggcg ccggaggcgg tggcatccac gtaaggcacc
     5941 ggcgcgaaga ccaggaacat cacgcccatc tcgtgcactt caccgtggcc ggatttcacc
     6001 gcccaggcgt ggcccagctc atggagaaac ttgatcgccg ggtagatgaa gaacagcagc
     6061 accaggttgt cggtggcgag gaggcggtcg cccatgttgc cggtgagttc gtcccagtgc
     6121 atgcccacca gcacgcccgc cggtaccacg gcgagcagcc acaggatcac cccgagaggc
     6181 ccgaacaggc gcgccaccca gggccagctg cggttgagga aggggtcggg atcccacagg
     6241 ggcaggcgga tcgacagggg attcaggaag ctctgcaggc gcttctgccg cttctggcgg
     6301 gcgccccgct cggccagctc ggccacgtcc ggcggcagat cggtctggag cagatcgccg
     6361 ctgtggagct tggagaggag ttcgatcacc tcgtcctggg tgggcgcatc cacaccggct
     6421 gcagccgcca cgtcctgcca gatgacgtcc agcgtgcttc ggccgtccat gcgctggagc
     6481 accgcgtagg caccgggcgt gaagcggtgg atcttgcccg acgcggcgtc gcgcatcacg
     6541 taccacggtt tgccgcggaa gcgctggcgc tgcacctggg cgtggccgcg cagctgcggg
     6601 cgcaggccgg cgacgcggta ccaggaggcg ctgaggaagg agccggccac ggcgtgtgcg
     6661 ccttaaggca gccagttcca ggcctgcagg cgcagccagt cgaacaggcg gtgggtccag
     6721 atccaggcgt agctgcgctc gccgacgacg atcttgccga cgccctccat gcccggccgc
     6781 agggggatgt cgccccggtc gaggcgggcc tccacgcgga acaggttctt gccctcctcc
     6841 gcctgggcca tggagatgtt gtgcaccttg aagtccaggg cgtcgccggc aaagccggtg
     6901 agcaccaggc gaccgccctg gccggcgcgc acgtaacgga tgtcccggtc gtccaccttg
     6961 aggatcaccc ggtaggcgtc caggggcgcg acttcgaaga gcagcttgcc ctgctccacc
     7021 ggcgagccga ggagctgggt caggtcgccg gtgacaacca cgccgtcgaa gggcgccacc
     7081 acctcggcgc gggcgagctt ttcttccacc agggcgagct gggattcggc ctcggccatc
     7141 tgggcgaggg acacatgggc gttggcgcgc tcgtgcttgg acaaggcatc ccggtagcgg
     7201 cgttcgtgct gctcccgctc ggcgctgtag cgctggcgtt cgatgaggag gtcgcgatca
     7261 tccaggcggg ccagcatctg acccttcttg accagcatgc cggcgcggat cggcgcctcg
     7321 cggatgtagc cctcgaaggg cgccaccacg gagcgctgca cttcgccttc caccaccgac
     7381 ttggcgctca cccggtaatc gccgtcaatc aggacgaccg ccgccagcag cacggcgatc
     7441 accgtgaccg cggcacggaa ggccgggcgg cgcggatcgc gcagatgccg ccagccgtcc
     7501 cggaccttgt cggcgagccg cccggcgaac cagcgatgga gctcgatgcg gctctccacc
     7561 aggggcccga ggagcgcccc gatggcttcg aagccgagga cctcgtcggg ttcgaagggc
     7621 tcgccggcat tgcgttcgaa ggtcagcacg ccgatgccct ggccgcggct gctgaggagc
     7681 accgagcaca cggcccgctg ggccgcgaag tcccggtggg cgatggcaat gccgcccgcc
     7741 gcggcgtcgg tgtcggtcgg ccagatcacg ctgcggcgct ggtcgagggc ctcgtccatg
     7801 gcgttctcga gggccaccac gaactggctc ttcttctcga agaaggcgga gtgggacagg
     7861 gccaccaggc gggcgcgtcc ggcgcgatcc acgccgaagg cgacccggtc gcaggcaaag
     7921 cggctggcca gatcgttggc aaccgccagg gccaggtctt ccaggcgcgc ctgctcggcc
     7981 gccacgccca gcacgtccag cgccgcacgg gcgcgcgtca gggcgagggc ttcgtcctgg
     8041 gcctggcggc gcaggaacac gctctcgagc cagccggcgc cccagtggat ccgccgcagg
     8101 acatcctgca gggccgcgtc cgggcgctcg gccacgtcca gcaccacggc gccgtggatg
     8161 cggccatcca cctccagcgg gtaagcgacc accaccagcg ccccttccag gccccgctgc
     8221 agggtgccgc ggcgttcgct caggcaacgc tgggcgcccg gcgcgaggta ggtgacgtcg
     8281 gtctggtcgg tgggccacac cccggccggg acgaaggtgc cgcctccctg ctccagcagc
     8341 agcaggccgg cgcgcacgtc ccccacctgg ctgcactgca gggccagcca gctcttgcag
     8401 aaatcggccg cgttgtcggc gctgacaaag gcgccccaca gggcgtcggg cgcgacaggg
     8461 acggcggcgt ccgccggggc agcggacacg gagcgggacg cagcgtgcac ggtgtgaaga
     8521 ttccgttcgg aaaacttgga cagccggcac cggcaggtgc gccgatggcg ccctgcccgt
     8581 gaccggccgg agggggaatt acttcagttc gccgtagcgg tgctcgtatt cgtccacgag
     8641 cttgctgagc atctgggaca gacgcttcgc cgcgaagggg ctgaggatga cgcgatgctc
     8701 cagcttgacc tcgtagtcgc cgccggtacg gtcccagctt tcattgacgc cgaagttgag
     8761 cacgacctcc tcccgcgtgg tggtcgcgtt gaccatgttg cagtaggtgc tcttgaggga
     8821 cgccgtgttc atgcggatgc gctggccttc cggggtctcg ggtactgctg cgttgtctga
     8881 cacgttgaat ctccgattgt ccaggggttc tcaccacgcc catcaggcgg gcgtggtgga
     8941 ttgtaccgaa gcgtctaccg ttactttcag gttcgcgttc ggattggtgg agctggtcgc
     9001 catattcgtc acgaaggcct tcttccagtt ctccttcttg aacttcggat cgctcgagcc
     9061 cacggtgaag ccggcgaagc tgctgccgaa atcgatcacc ggcgcggtcg gcggtgtggg
     9121 tgcggtggcg aagttcacct tgaggccagc cgggacgtcc gggatttccg gccgcgccac
     9181 acccgcgatc ttctgggcga cgcgggaagc gtcggcagtg gtgagcttgc cgtcgccgtc
     9241 gatgtcgccg atcagcagcg gatcgatgta ggaccacgca gcgagcccgc tgtcgagctt
     9301 gagcgagttg cgctgcagca gcgtgacgtc gtccttgtcg agcttggcgt tgcggttgcc
     9361 gtcgccgatg tagccgacca cgtgcagggc gtcgtcgtca gcccggtcgg cggccgtctg
     9421 gttggcaaac acgttgccga tgtccaccac ctccaccgca ccgtaggccg ccgtgtcggg
     9481 caccctggcc accaggttca ccagcgtgag cttgccggcg gcgatgggcg tcgccgaggc
     9541 gatggtcacg gtgatctggc cggccaccga ggtatccaca ttgaggctcg cgccggacgg
     9601 cagcccggcg ccggccacgg cgccggtgat ggccagcagg ctcggatcga agcgtacggt
     9661 gaagctcagg ttgcgcacgt cgccagcact gctgaggctc agcggcaggt acttgccggt
     9721 ggcgggcaca tccacgctct ggcccggccc gcggacgaag tccggcaggg acaggcgcag
     9781 ggccggcacg ggaccgatgg tgaagcgcgt ggtgtagtcg tcgccgccgg tgccgtcgcc
     9841 attgccgtcc agggcgccga aggcgctgtg gaaggccagc ggaccgctct tcagcacgag
     9901 gctgtaggtg tcggcggcga ggccgctgcc ggtggcaagg aagcgcaggc ccttgccgtc
     9961 ggcgtcgaac accagggagc ccttgaccag gctgccgccg ctgccgctca ggaccacgtc
    10021 ggacacgccc aggttgctca gggtggagtc gtagaggttg atcaccgagg cgtcgaacgg
    10081 gtcgttgaag cgcaccgcga agccgttgct gtcagacgtg aagctgcgca cctgcagggg
    10141 caccggcagc accgcgatgg ccagcgggtc aagggcgtag ctgccgtcct cgtcggtggc
    10201 ggtggcgcgg atctgtacct gaccgagcac gctgccgtcg taggtgtggg tgatctggcc
    10261 cggattgccg gccacatcct gcacctggcc gtcgccccag ttgatgtgcc acaggctgag
    10321 ggtgtcgtca cccggatcgc tgctggagag ctccagggtg tagctctgac ccgcatacac
    10381 ggcgggcagg ccgcccgcct gcagggtggg cgcgacattg gccacattga cggtgaagct
    10441 gcgggtcgtg gtgccgcccg agaggtcgct gacgcggacc gtgaaggcgt aggcggcgtc
    10501 gccgtcggtc gccttccagg tgagggcacc ggtgagcggg tcgatctttg cgccctgcgg
    10561 ggcggcgtcg agggcgtagt tcaggacatc gccggcatcc acgtcatgac cgacggcggt
    10621 gacggtgaag ctgtcccctt ccttgacggc tgcgtcggcc agggcatcca gggtcggcgc
    10681 gtcgttgacc ggggtcaccg tcacatccac ggcgcccttg gcggtggcgc cgaggctgtc
    10741 cgccaccacg taagtgaagc tgtcggcacc gttccagtcg gcgtccggcg tgtagcggac
    10801 ctggttgcct tcgatggcca gcgtgccatg ggtggcaccg gtcacgctga caaggctcag
    10861 gacctgccct tccgcgtcgg agtcgttgcc cagcacgtcg atgaggaccg aatgatcctc
    10921 gtcggttaca gcgctgtcgg catgggccac cggtgcggtg ttgtggagct ggacggtgat
    10981 cgccacgctg ccgtcgacgc tggccccgtc accgtcgctg acggtgtagg taaagctgtc
    11041 ggcgccggtg aagccggcgt tggcggtgta gcgcacctgg ccggactcga tgacgacggt
    11101 gccgtgggcg gcgcccgaca cggcggtgac ggcaagctgg gcgctgtcct tgtccgtatc
    11161 gttggcgagc acgtcgatga gcaccgactg gccggcctcc acggtcgccg aatcgggatt
    11221 ggcgacgggc ggatcgttca cgctgttcac cgtgacattc accgtggccg aggcctgggc
    11281 ccccacggaa tcctgcatca cataggtgaa gctgtcggca ccgaaccagt cggcgtccgg
    11341 cacgtagcgg atctggccgg cgtcgaggct gaccgtcccg tgggtggcca gacccacgct
    11401 caccaggctg agggcctgac cctcggcgtc ggtgtcgttg gccagggcgt cgatgaggat
    11461 cggcgtgtct tcgtcggtga cggccgtgtc ggcgttggcc accggcggcg tgttgtgcac
    11521 caggatgtcc accgtcacgc tgctgccggc gttgccgccg tcaccatcgg tgatcgtgta
    11581 cgtgaagctg tcgccgcctg catagccggc gtcgggcgtg taacggacct tgccggactc
    11641 gatggcgacc gtgccgtggc tggcaccggt ggcgccggtg atggtcagca ccgggctgtc
    11701 gatgtcggtg tcgttggcga gcacgtcgat gagcaccgaa ccgccggaaa gcaccgccac
    11761 actgtccgga ttcgccaccg gcgcgtcatt gaccgggcgc accgtgatcg ccacggaggt
    11821 ggtggcggta ttgccggcct cgtcgcggac cacataggtg aagctgtccg caccgaagta
    11881 gttggcgctc ggcgtgtaac ggacctggcc gttttcgagg gtcacggccc cgtgggtggc
    11941 accgtcggca ctcaccaggg tgagggcctt gccttccgga tcggtgtcgt tggcgagcac
    12001 attgacgagc acggaggcgt cctcgtccac cgcggcggta tcggcattgg ccaccggcgg
    12061 ctggtcttcc acctcgccca ccttgagcag gcggacgttg tcgacggtga ccgaactggc
    12121 ggcttcgccg aagcccagga ggtcgaagta cagggtgacg gtggtgcccg ccgcgacgcc
    12181 ggtgaggtca atgctcacgg tgatcggacg gccggacggg aaggtgccgc cgttacggtt
    12241 ggtgaggccc gccgcgctga cctggtcggc cgcatacacc gtgccatccg cctgcaggtt
    12301 gaacagggcg tcgctgccgg acaggggcag ggtgccgccc accggcttgc cgtcggcgcc
    12361 gatcagggcc acctcgaagg catccggcgg gccgtcgccg ttggcgacga aatggctgga
    12421 caccagctgg aagcgcagcg tcgcggcatc cgccgggacc acgaaggtct gggcgaagcg
    12481 cgaggcgagg cgcgggtctt cggtgaggac gccggcgcca gcgctgacct tggcatcgcc
    12541 ttcggtgatc cagccgtagt cggtcgctgc cggatcggcc acggagaact ggccattgac
    12601 gatgccgtcg tgggatgcac cggaaatcag aattggcgag gacgtgacag gcgacgtggc
    12661 gtcactgttc tgactcacga agtagagcag accgcccagg gggtcggtgg aaccttcacc
    12721 gtctcctgcc agcggggaca ggttcaagac ttgccgtccc gcacgattag acttgtcgag
    12781 cgcatccagg tccaggtacc aggacagcag cgcgtcggtg ctggacaggg cgaccgtcgt
    12841 gtcgggattg tccatggcca gatgcagcgc cgcggcctgg gaactgctca cgcggatgtt
    12901 gttggcgttg gacatgaagt tcgcaccacc atcccgtggg gcgttcgtga aggggctgta
    12961 gatgtactcg tcgaacagcc ccatgttgtg ggccagttcg tgacctacca cccaaccgta
    13021 ggaccgcccc atatccttgc gggtgaagcc gaccgacagg taggcgcgca ggatgccatc
    13081 caccaccagc gtcatggcgc tctgaccgcc catggcaccc gcgcgctcgg ggttgaccac
    13141 gcgggacagc agatagcgct tggaggcgat ggagatgtcg tcgcggatca ccatgccgcc
    13201 gaagctgcct gccccggcac ccgccttggt gagggtcgac tcgaaggtgt tgtaggcgaa
    13261 gtcgagaccg gcaaaactgc gcgaggcatc gtcattcggg tctggcttcg caaccgtatt
    13321 cacccacggc tggttgatca tcacgatcgg attgagacca gtctcgccat acgaaaactc
    13381 cgtctgggcg atgttgttct ggtagatcgc gctggccccc gtgccaaacc actcgttgaa
    13441 tccatccacc acgtcctgcc attgcgcgtc ggtgatcacc gcatccggga acaggttcag
    13501 gaagatgttg tactttccgg cagcaaagcc ggtcggatca tgcacgaccg cggtgagggg
    13561 gttgttcttg tagttctccg cctcggtgcg gatgttctgc accatcgtgc gcagggttgc
    13621 cagggacacg cccacggtct gctttggcac cgaatagccg gcgaccttga tcgtccccag
    13681 cttgcgccca ccctcctcca cctgataggt ggcgaaatcg ggcaaggcgt tcacataacg
    13741 gccagtgtag gtgaggccct ggacgatgcc cccggattgg gacacgccga acagattgta
    13801 ggtatcggac gtctccttga gctccacatg ggccgcattg tcgaagctca gggtgcgggt
    13861 ctttccgtcc atcgtgtcca tgaaggtcca caggccatcc gtcgcgttac cgtcggcgta
    13921 gtccggcatg tagaacaggt ccaccgcctc gtcgcggatg ctgccgttgg cgagggtgat
    13981 ggtgaagtac agattggtct tctggatggg aatgtccgca tccttgtagg tcttgatgaa
    14041 gtccgcattg aacttggact tcaggttgag cttcagatgg gcccccggcg ccaggacgta
    14101 atcggtgtac agcggatcgt gggcgttgtt gtcattgagc accacatcac cgctggtgcc
    14161 ggccaccacc aggaagctgt tcgggtcggt cgtgaccgtg acggtgaaag cctggctgaa
    14221 cgggttggtc agatccaccc actgactgct caggcctgtg gtcggaccgg tggtggtgtc
    14281 ggtgaagaac accgccttgt cggacggatc gagggtgctg tcggtgatca ggcccttggt
    14341 gaattggacg tgatccagaa gcacctcggc atccgccgtc gcaccgctgc tcatggagaa
    14401 ctcgagcagg cggccggcgc cgcggaaggc ggtgggcacc tggaaggtgt aggactggaa
    14461 gttggtgccg gtgttgtcca gggcgatgct gcccagctcg atcggtaccg acgaatccgc
    14521 cagatagccg cgcaccagaa gcttctcgcc cgccttgccg gtgagcacct tggcgtccac
    14581 cgtcaggaac tcggcccagt cgggcaccac catgcggttg tgctcgatgc ggtagttgtc
    14641 gttgtcgagc attgcggcgt gggtaccgtc cgccagcgtg accagacgat cgccgtcgat
    14701 cagcgtccgg gtcttggcaa acgagccgcc atggaaggac cagcccggca gatcgccggt
    14761 gaggcgcgtg gccaggccac ccgccgagta ctggagcagc cagctgcggt tgagggcgcc
    14821 ttcctcgaaa tcgccattga agatactcgg tacggccatg ccggaaggat tgggcgcctc
    14881 agtgttgtcg gtcgccaggg agaccttggt ggcgctcatg ggcccggagt aagtcacacc
    14941 gccctggggc gcaaaggccc agccggtgcc gatgccttcc cacggtccgc tgccaagggc
    15001 aaagctctgg gcgttgtacc agggcacggt gacgccctgg aactcggtct tggtcttgtc
    15061 gtagagacgc cggtagaccg gctcgccgcc cgcctggaag gtggtggcgg agaggtccat
    15121 ggtgccgccg taccagcgcc acacacgact gtggggacca ccggcgcgga attcgccgct
    15181 cgccaggagg gacgcgctga tgagcccgtc gtcccgggtg aaaccggcgc ggccggtgag
    15241 attgacgctg tagtcgaagt tcggcacgta gcgcccgttg ggcgtggtga cgatctgccc
    15301 gaagcccatc ggagtattgc tggccaccac ctggtagtag gcgtcggcaa acgcgatgtt
    15361 gctccagcgc tgcacgtccg ggtcgaggaa gttgccggtc gggatgacgg cgttggtggg
    15421 cagcatgccc cacagggaga tcaggttgcc gaccagcccg gatacggcgc ccgccgctgc
    15481 accggccgcc tggatgtatt gcgcgatggg gatgtccagg gacggctggt tgaagtcatg
    15541 cacgtccagc gcggtcatgt cgagggacac cgtcggcgca tagacgccaa aacgctgggc
    15601 cagctcgcta ttgaccacgg tgccgcggct gtggccgatc aggtggatgg gcgactggaa
    15661 gatcttgccg cccgattcct cgttgagctg agccagggac gagtagaagg tatccgccgc
    15721 cgcttcgctg aagccatccg tggggaagtc ggattcgtcg taccagtcgc tcaccagcgc
    15781 aatgggccgg ccattcaggc tgggcatgcg cagggcatcc accagatcgt ggcccaccag
    15841 ggcaccgctg ttggcgccat cgtagaagat gatccagtgc ccggaggact tctggtagcg
    15901 cgccgccacc ccttcaccgc catccttggc gatggtttcc gccatctggt agaaggacga
    15961 cagggcgtcg gccaggttgg ccggctgggt cggcgagagc tggaagccgt gggtcagcac
    16021 ggtgacgctg ctgtagggct tgctcggatc cttgaaggcc tcggtcttga acacgcgcca
    16081 gtcgcgggcc accacggtgc cggacggcga caccacctcc accccccagt aatactgctg
    16141 accggcggtg agcttgaggt tggcgggcag ctgcttgatg aagatcgcgc cggtggaatc
    16201 cgccgagctg gcgtcaagca ggtcggacga gaagatccgg ttggggttct ggtccgtcga
    16261 gcccagatcg gtggggaaca gaccgtcgct gccccggaag gtgctcacgt agagccgcgc
    16321 cttggcccct tcgaagccct tcaccatcca cgagaaattg gggttgagat tggacgtgga
    16381 ggcatccggc gccagcagtt ccagcgaagc gccgccctgg gtcaccagcc cctggttgaa
    16441 gccgacgccg atgggcccgt tggtggcccc cggcgggacg ccgaacaccg gatcggtgaa
    16501 caacccgccc ttcttgagca tcttgaagtt ggcgcgcaca tcgatggacg gatcgatgtc
    16561 gtccactgcg gtggtgccca gatcgggact gccgctctcg acgacggcga tcatcttggc
    16621 cacatcgaag gccatgacga cgcgctggcc gcggaaggcc gcgtacaggg tgttgccgtc
    16681 cggcgacagg accgcgttgt caggccagga catggggatc tcgcgggtcg cgccgacgag
    16741 ggtcgggttg ggcccgaggg gatcgcggat gatgccgatg ttgccacccg cggggtggtt
    16801 cgggtcgtag ttcggatcgt gggacggcac gtcctggatg taggtgttgt acccgacgac
    16861 gaaggcgtac ttggtgtcct tggacaggac cacggaactg gcgttgttca cgtcgaacca
    16921 gtccgtgatg gcaccgaggg tcagggtcac ggtggtctgg gtgtaattgc ccacgtcacc
    16981 cttgacgatc accacgccct tggagtcgga cagacggttg gtgtacatca tcacgttcgg
    17041 gtcgttggtc gccgatacgc cgtagggctc ggaacctgcg tccagcgtgg ccaccacctg
    17101 accgtacgta ctgctgccct cctccacgtc gatgacgaag accttgccgt caccgccgcc
    17161 ggggcggccg aacagtgcct tgtccggcgc cgcgacgaac agacgcttgc cgttggcgtt
    17221 gatggccatg ccgcgcagac cggacggcgc cggattcacg tcgatggtct tcaccagggt
    17281 gttgtaggtg gaggacgacg gatcgatgtc gatcacgtac acgcgaccgg cgacctcgtc
    17341 cgacacgtaa gccttcttgc cgcccttggc gatggcgatc cagaagggct gggcgccggg
    17401 caacagcgga atgctgctga tgccgttggc cgtatcgccg tccacatcga cctgctgcag
    17461 ggcgatggtg tcgaccagcg ccaccgcgcc gttgtagcgc agtgtcacgt aagcccgggt
    17521 gttgtccgaa ctgatcgcca tggcccgggg cgccgggaag ttgccgctca cgttgatgcg
    17581 cgccaccaga tcctgagtct gactgtcgat ggcaaagacc tgattgcccg acatgccgcc
    17641 gaagatgtag cgcgcgtcgt aggacagctg aaccttgttg ctgtccagtt gttccaggtc
    17701 gtcccaggtg ccgccgttgg cgtagtagat gggccgccgc acgaagatct tggcggtgcc
    17761 gatggagacc gtctggggca cttcgacgaa gacgtcgcca tcggtgctgg tcgcgctcgg
    17821 cgaaacctcg aacgtgtgcc cgtccggggt ctcgaacacc accttgagat cggcgatctg
    17881 actgcccttc ttggcgaagg ccggcgcggc cggattgtcg taggtgaagc ggtcgccctt
    17941 gatctccacg aacggcacca cattgcccga tccgtcgtcc ttgaagagga agcgggccga
    18001 cgagacgaag ggctcggtgg ccggcgaggt gctcgggctg taggggttgt tcacctcgat
    18061 gcggttgtcg ctgaccttgc ccggatccac ctgcacgccc accttggtga ccgtcggcag
    18121 accctgggcc ggcaccgaga tgatctgcag ctcgtgataa ccgatcaggc aggtaagcat
    18181 gaagcccgcc gtcaggccga aggcggcccc catggccgcg ccaccgccca gggacgacag
    18241 catggcgaag gacgagccgc cgtcgttgat gcccaccggg aaggttgcgt aggtctgacc
    18301 gcgcacctga ctgaagctgg catccggcgc gatgccgcag gtgtaggtgc ccgacaccgt
    18361 gacgccggga tacgggggcg acatggcgcg ggcgtaaccg tccgcgccca ccacgccggt
    18421 ctcttcctgc agccagatgg gctgcttgga gccatcgggc atcggaattt cggcgtagcg
    18481 catgaacacc accttggtgc ccgcggcgat gccggtggtg gggatggcga gctggatcgg
    18541 cgtatcgagc ttgcgatcgc ccagatccag cttgaaggcg cctgccaggg ccatgccatc
    18601 gggcatgccg gcgggcaggt cggcggcctt gaccgcttcg atggacaccg tcgtgttgcc
    18661 acgcaacgcg cccggcgcca ccgcgacgat ggacccgtcg gcgccctgca cgatgccgcc
    18721 cagatcgccc agcacggtgg gcccgacgaa gggcttctcc accttgaccg ggatgatggt
    18781 ctcggcgccg ccgttgatga ccgtgacggt cgcctcgcca ctctggcggg cgctgatcag
    18841 cccgtcagcc gagacgctga tgatctggtc gttgctcacg tagtagtgcg tgccactgga
    18901 ggccggagcc aggtccatgg cgccgtacag attggccagg atctgacgct tgcccacgtt
    18961 ctcggcgagg ctcaccgcgc cgggatagac ggtcatgccg cccgcgtaca ggtaggcctg
    19021 cacggtgtcg gtgggcacac ccacggtgaa cgcggtggcc gcgcgcagac cggcacgctc
    19081 ggcgatgagt acgccggagc ccttgcccac ggcgttgatg cggccgccgg caacgaggct
    19141 gccgatggcg ccgtcggtgc tcatcagggt cacataggac gccggcagtt ccacgttggc
    19201 ctgatcttcg aagtcgccga tcaggctgat ctggcgacct tcgcccacgg cgagccgcag
    19261 gttgcggtcg acaaagtcga ggcgggtcag cgccgcgttg ctgatggtga cggcgatggt
    19321 ctggatggtc tgaccaaaac cgtcgtcggc gcggatggtg aagtctgcgg tgccgatgta
    19381 gccggcctcg ggcgtgaaca ccacggactg gccgtcgccg gacaggtgcg ccgtaccatg
    19441 ggttgagccg aggagggtga agaaggtgcg gtcgccttcc ggatccttca cgaaggtgga
    19501 caccggcgca ttcacgtcca gatccacgtg ggtcttcacc gtgccggcca caacgaccgg
    19561 cgcccggttg ggcgtgaagc ccgcgttctg catgaggagc tgcaggtcgc cgctttcgat
    19621 gacgccgttg ccgtccgcat cgagggcctc gctgtagccg gccccgcccc gcaccgcgcc
    19681 cttggcggcg gcgatggcga tggcgtcggc cccgtcgacc ttgccgtccc ggttggcgtc
    19741 acccgccacg aacaggctca gggtgtagtc acccacgccg ctgccggcca cgtcgatctg
    19801 cgccaggccg gcggcatcga gcttgaagag ggcgaaggcg cgattgccca ccaccgacga
    19861 ggcgatggcg gtgaggccgg gcatgaccgg caggaccggc gcgaggccgt tatggccttc
    19921 gaccacgatg cccagataca ccgagccgct cgcggtggag gccagctccg acggacggag
    19981 cgtgaaggcg tagtgatcca cctcgcccgc gatcaggctg ccattcaccg gagtcgcaag
    20041 gtaattgacc gaggacccca gatcgccgga gatgggcgcc tggccgactc cggacggacc
    20101 ataggtgtac agggcccctg ccccgctgcc ggtgaccagc gtcaggcgac cgagggcgcc
    20161 cgcttcgtag ccataccgca gcgacgtagc cgcctccagg ttgcggactg aaacgagccg
    20221 gccctgggcg tcgtagtcgt agaggtactg acgcccgtcg gacagggcgg cccgttcgat
    20281 ggccccggcg tggttgaggg tgaattgcac cgtctcgccg gtactggcca ccagaccgct
    20341 gtcgctcacg gccacatgca cgccgtcggt gaagcgcacg tcgtcgatgc ggccgtcggc
    20401 gatgctgtag cgggtgccgt ccggcgccac caggatgtag tccgtgtcgc cggaattggc
    20461 cgggttgtag cccacgccgc tgaccagatc gtagtagctg gacccggccc gctgcagctt
    20521 ggtgtcgggc acgacgagct gccagccgct ggcggattcg gcctggaagg ccggggtgta
    20581 ccaggtctgg ccggggatgc tgtgtttctg cggcacgaag gtgaaggctg cccgctcacc
    20641 ggacggcagc gtcaggacga cccgctgacc gtcctggaag gtgcccagtt cacccgcatc
    20701 ctcgcgcccg gtgagggcca cgtcggtctg cagatccaca tcccggaagg agagccgcca
    20761 gccgttgccg aagctgccgg cgacggaggt gttgagcgaa tcgtagctgc gcaggaagtc
    20821 gagggtgtgc ccgccgacgc tccagctgaa atcggcttca ttgcgctgca gacggcccgt
    20881 cttggtggcg gaatccactt ccacgtcgac gctggcttcg ccgcgccgcc cgcccaggtc
    20941 ggtgacccgc aggcgcaggg tgtacatgcc gttggcgagg gtcgccggat cgagctgcat
    21001 gaaggcggcg ttgttcaccg cgccatcgcc gctggccagg gtcgtgtagt gctcgtcgcc
    21061 gtagcgcgcg atctccagcg tccaggcatc cacattggcc tcgccggtgc tgccggtgat
    21121 ggccgtgagg tcggtgaggc gcgcgaaggc cagctggctg gcgaaggacg gcgtcggggc
    21181 ggatttgtcc tggggatcgc gcacgcggat cacggacgtg gtgctgccca ccaggccatc
    21241 cacgtcggtg gcctcggcgc gcagggcgat cttgcccacc tgggtcgcct ggacgtgggc
    21301 gcggccgaat tcatccagcg gaagctcgac accatcggca aacagacgca gtccggcgat
    21361 gttgccaaag ctctcggcga tgacgtggac cagcacgtcc tggccgggca ccgccgggaa
    21421 gctgggcgtg ttctcgatga tcacgctggg ggccggcgcc gtgagggcgg cgcggatcac
    21481 cagcggcagg gcggtgctcg tcatgccgtc ggacacggag gccatgacca ggtagtcccc
    21541 agcctgaccg gcgcccggcg tccagttgat gacgccagtg gtcggatcga gggtggcccc
    21601 ctcgggcagc ccggtggcgc tgaaggtgag cgtggtgccc gaatcggcgt cgctgccacc
    21661 aagctggaag ctgaggggcc ggccgaggag cgccacgtgg ttggagacgg tcagcaccgg
    21721 cgcccggttc acgttggcaa tggccaggct gacggtggtc tggctggtgg cgccggcagg
    21781 atcgcgggcc tcgaaggtga agctgtagtc acccgcctga tcgtaggtgg gcgtccactt
    21841 gaagaggccg gtgcgcgagt cgaagcggga gccgtcgggg agcgccgtga gggccttgaa
    21901 gacgaccgga tcgccgtcca cgtcgcccac ggtgagctgg aactggacct gcgcgccctc
    21961 gcgcccggtc tgcgggaaga gggacaccag gatcggcgcc tggttggtgt tctgcaccga
    22021 gatcgtgacg gtctcggcgc tctccgccat gccatcgctg acggcgaagc gcacgttcgg
    22081 gtagtcgccc gcactgaaga tgttgggcgt ccaggtgaaa agccccttga ccgcgtcgaa
    22141 gctggcgccg gccggcaggt tggtgacgct gtaggtgagc ggatcgccat ccggatcgac
    22201 ggcggagagc tgcagcgaga agggctggcc ttccttgagg cttaggttgc cgatgggcga
    22261 caggaccggc gcggcgttgc tgtcacgcac cttgatgcgc accacctgct cggtggtgtt
    22321 gggcgccgtg gcaccactgt tgccgttgtc ggtgaccttg aaatgcaccg tgaaggtctg
    22381 gcccagcacg gtgctgtcgg gcgtccattc caccagggcg cggccgtatt ggggctgcgg
    22441 cgtgatggtc aggccggccg gcgcggcgtc tgcggtgaag cccagcgcat cctggtccag
    22501 atccttggcg ttgatgtaga agctgagctt ctggccaacc agggccagct tgtcgccgat
    22561 gggcgcgatg accggcgcct cggaggcgga caccaccgtc aggatgaagc ggcgggtcag
    22621 ggactgggcg tagagggcgc cctgtccgtc gccgttgtcg gtggcggtga ggtcgatctc
    22681 gtagtcacca cggtcgccgg aatggggcgc gatgacgaac ctgccggtgc cgtcgccgtt
    22741 gtcggtgaag gtggcgaagg cgggcaggcc cttgacagtg agcgtcagcg gattgccatc
    22801 gggatcggta gccgtcacgg gcagctcgaa cactgcgtcg cgttgcacgg tctgggtcgg
    22861 gatctcggcc atctccggcg cccggttggc gttgcggatg gtgatgggca cgacgatgtc
    22921 cgaggtgaga ggcgtgccgg tgccgttgcc gtcgtcggtg gcggtgaagt gcaccaggta
    22981 gctgcccgcc tgatcgtagc ggggcaccca cttgaacatg gacgtcaccg gatcgaagct
    23041 cgccccgtcg ggcaggccgg acacggtgta gctgaccgtg gccgcggccc cttccagttc
    23101 ggtcagggtg ccgctgggca ggcggtcctg ggcaacgtag ccggggttgt ccgggtcgaa
    23161 ggcgaaggcg cggaaggcca gggtctggcc ctcgaagctg atccacgatc cgagttcgtc
    23221 gaagaccggc gccgcattca cgttgaggac gtggaacttg acggtggtct cggtgcgctt
    23281 ggtgccgtcg ctgacgccga acttgaggct gtagtcgccg gcctggatga agctcggcgt
    23341 ccattggaag acgcccgtat tcggatccag gaaggcgccg gcgggcagga cgtcggcgta
    23401 gaagctgagg gtgtcgccgc tgtcggcatc cgtggcatgc agctgcaggc gcaccggatc
    23461 gccttcgcgc agggtgcgct cgggcaccgg gctgaggacg ggcggcgcgt cgtccggggc
    23521 gatgacgatg ttgaaggacc gcgtcaccgt gctcttgccg tcgctgacga tgagcttgac
    23581 gttggtgtac gtgccggcgg acgtgaagtc gggcgtccac accagggcgt tgagctgcga
    23641 atcgaagacc gcgcccggcg gcaggttgtc ggcgtagtag tgcaggaagt cttcgtccac
    23701 atcttcggcg gcgaggccga cgagcaggct ctggccttcc ttcccgtcga tctcggcggg
    23761 caggtcggag acattgggcg ggtggttggc gcccgccacc tcgatgttga agacctgggt
    23821 gtcgaagcca ccacgggtgt cataggcccg cagggtgacg gtggacgtgg cggggctggc
    23881 gacgttgggc agccagctga ggatgcccgt cgtgctatcc acggtgaggc ccgccggccc
    23941 atccaccagc aagtaggtga tcacgcggcc gtccggatcc tgggccaccg cctgatagcg
    24001 gtaggcctga ccggccgtgg cggccgtcac cggcgtcgag gtgaaggccg gcttctggtt
    24061 gggatagggc acggcgtaga tgccgtggcc caggctgatc tgctggctgg cgctgttggt
    24121 gatgcccacc gtgaaggtgg cgatggatgc accggccttg agcacgccgc cgggcagctt
    24181 accggacagg tcgatgtacc acaggccgtt cgctccctgc gggctcaccg cacccatggg
    24241 ccgtccctgg aagaaacggg ccgggtcgat gaccagcgcg gccggcacca gcaggtcgta
    24301 gtcggcggtg ttggtgacgg tgatgtcgta ggacaccacg ccggtgccgc ggtcgctgcg
    24361 cacccggtcg aactgcaggc tcaccaccga ggagaagtcc tgcaccgcca cgaagctgga
    24421 gatgtaggta ccgcccaggg ccatgccggc gctgctgcgc acggtgccaa gcacgcgcag
    24481 atcgtagaga tccgggtcca gaccggcgac cgtgagggtc acgcggcggg tggtggcatc
    24541 gtaggcgacg ctggcgatgt ccaccaggct gccgttctgg cggaccacct gatagacggc
    24601 cgggtcgagc acggaggcgc tgtccgtggc gctgcccgcg tacatatcct ggtcgaaggt
    24661 gaccgtcagg gtcgtcagcg gcagggtcac cacggcctgg tcgggcggat tggtggcgac
    24721 gacccgtggc gcgacaatgg ggctgatcac gtcgatctgg tgggtctcgg ccaccagcac
    24781 gcggccgtcc ttggtggcca ccacggtctc gccgcgggaa ccaccccggg cgatctcgac
    24841 gctgcgcttg gacaccagat ccaccatggt gagggcggcg ccctgctcgc ccttggtgga
    24901 attggcggag atgaagagca ggccttccag gcgggtgccg acctggccga aggcgatgga
    24961 atccacctcg ccgtccaggc gcacctgcac ggtggcgcgt ccgcgcttgt cgaaggtgac
    25021 cacgtcgccc cggtcgggcc aggtggtacc ccacagggtg ccgtcgggcg cgaaggcgag
    25081 gtcatcgacg cgcacgtcgc tgaagtgacg gaacgtcgcc ttgaccgggt cgaagatctc
    25141 gatgccgtcg ccggacgaga cgtagatctc acccgtgccc gccttgatcg ccagggcctg
    25201 ggtgatgccc gtgccgtagc gcgcgagcac cgcgccggtg gccagatcca gcttgagcag
    25261 gtcgttgccg ccggtggtcg cccacagctg gccgttgtcg tccagggcca ggtcgtagat
    25321 ggggtcgtcc agttcgacga agggcgtgag ggccttgccg ccgtcgtggg ggatgcggta
    25381 cagccagccc cggttggggc cgccactggc gagcaccgag ccgtcgggca gctccagcat
    25441 ggccagcgca ccgacaccgg cgccgctgct gacgaagtcg gtggcgaagg cctgggccgt
    25501 gaagggccgc tcgacctcgg tgaactcggg cagcttggac aggggtgtgg cggccggcgt
    25561 ctgcgactgg gcctcgatga acagcggatt ggtcggcggc gtggccggcg ccggcggcgg
    25621 cggcagatcc tgggtggtct cgccgaagct cggcaggccg cccaggttgg cgccggagcc
    25681 gtcatcgggc gcctgcacgc tgcccggggg ttgctcccgg ttgcccgcgt tgtccgtggc
    25741 aagggccagg aattcgtagg tgtggccggc cttgccggaa tagacaccga aggtgtcggt
    25801 ggtctggcgc agccagatct tgaagtcgcc gccatcctcg gccacgtaca cggtgacgtg
    25861 ggcgacgccg gagccgcggt cttcgtccgc gctgctccag cgcacctcgt aatcgttgcc
    25921 gccggcggtg accggcgtgg cggtgagggt ggtcaccggc gcctgggtgt cgagggtctg
    25981 ggtgagcttc tgggtctctt gcggggcgat ggcgttgaag cgcaccacgg cctgggcgga
    26041 gatctgcgtg ccggaaggca ggtcggcctt gggctggacg gtgtagccga gctggccggt
    26101 accgcggccg tccgctgcat tgggcaggag caggccgcgg gtggcgtcct gcaggacctc
    26161 gccggtgtcc gggtcgatgg cctggaagac ccacgaggcg gtgctggtgg tcgggtcgat
    26221 gcccgccgac acgcgcagga tgtagccctt ggaattgcgc aggtcgaagt cgccctggaa
    26281 cagggcgcgg ccgctcggga tgtggatcgt cacgtcaccg agcttgatgt cacccaggcg
    26341 gaaggtggtg ggatccaggg acggatcgag ggcgctggcg acacggatct cggtgacgga
    26401 gctggacgcc gcagcaggat tctcgaactt gacggtatag ggcagcgcct gaccgacggg
    26461 caggaactga tcgacgccgt agccttgcgg gccggagatg gacgccagct ggctgtcgct
    26521 gatggcctgg gcgtagaggc gcgagaagtc cagcgcggtg agctggttgg acgcgtccac
    26581 cgaggcgtag tcgggcagcg caatggtggc agcggacgcc ccgtccttgt tgaagcccac
    26641 gtagggcacg aagatgttga aggtctcgaa gtaggtcggg tgcgacaggt tgaggttgta
    26701 gtccgcgtac ttgggcaggg ccggcacggg gatgtcgcct tccaggcagt cgctctcccg
    26761 gtgctcgtag tggtcgatgg cggccagggt cttgggcgtg tcgccgtacc aggtcttgat
    26821 cttggcgaaa aattcgacga gatcgccggt ggtgcggatc tggtcaccca cggggccgag
    26881 caggatgccg gtcgccaggg tgctcatcag gctcatcacc ttggtgttct gacggatcgg
    26941 tggcgcctcg tccaccggac gcaggatgcc cacttgctcg agggcgccca ggtaggcatt
    27001 cacccagccg tccgcatcgg aggccaggtt gcgcagggcg atggcggcgc gcttctcggc
    27061 gagcgtggtg tcatcggtgc cggtggcggc gattttggcg tccaggtcgg tgtcgccgag
    27121 gatggcggcg cgcagcttga gggcttcggt ggtctggtcg gcgatgaact cgtcgcgggt
    27181 catgggggtg gcggccccca cgatgttgaa gcggtagggg atgaagggga tcacgcaatc
    27241 atccggcaga ccgtcttcgg agttggcctg ctgccacaga tcccagtagg ccggcagcgc
    27301 cgtctgcatg gactccggcc cgcccgccag ggcctggatc ttctccaggt ccgggtacat
    27361 ctcgtagagg ccgacccgca gggcatccca gttgagggtg gacagggcct tgagcagcgg
    27421 gtaggtggtg acgttgaaag tcgccccggc atagccgccg gcgatcacgt cgtaggcata
    27481 acccgacgac aggatctgcc cggtggtgtt ggtggcggaa tccaggctcg cccacggcac
    27541 gtcggcgccg ccggtgtcgg gctggccgcg caggtcgttg tagtacttga ggaagggcag
    27601 gttgtagacg tactggttgt tgcccatctc cggcacgccg aaggtgaagc gcacgtaggg
    27661 cgtgtccacg ttggtgaggc tctgcagggc cacgccgtag gtgccggtct cgccggcagc
    27721 cagcacgcgc gggccgccca ggccgatggt gacgtcggtc tccatggccc gctcgatgag
    27781 gaagcggtag gcgatgatgg ccttgtcgcc gctcgggttg atgacctcca ggtcatacag
    27841 gcccagcggc gcgttggtga agtcgaaggt ggcgcggatc caggtggagt cgatgacgtc
    27901 ccacttcacc ggttcgtatt cggccacgtc gggccgcacc accttgagga tggcgttgtc
    27961 ggcgaactgg gcgccgcgca cgtcgaaggt cacccagcgg ctgtcgccgc cctggtccac
    28021 gtggacgctg gtgatggcga agggtacgac ttcggcgagc acgcgcaccg gggtgttcgc
    28081 ctgggtttcc gcatggccgc ggatcaggac gtagtaatcc cccggcttgg tgaagggcac
    28141 cacggcggtc tggttggcct gcagcggatt gtcgtaggtg gcgtcgtaat ggaagccgtc
    28201 cggcacgtcg ccgtagcgga tgaagatctc gttggcggcg tcctggtcgc cggtggtgag
    28261 gctgaccttg agggtctggc cctgccccac ggtgagcttg tagaggcggc tctggccggt
    28321 ggagagggtg gtgtcctcat acacgcccag gtgcagttca ttcaccgtga cgttgagcac
    28381 attgggcgag gtggtgaagt tgttggcctc acctgcagag cggtacgggc cctcatagac
    28441 ctcgttgtag atgtccggcc gcacgatgac ccggtactgg cccggcttgg tgggcggaat
    28501 gagggcgtcg aggctggccg tgtaactgcc gtcctggccc agcacgccgg tgtgggtgac
    28561 cttgccgagg aggcggtcgc cgatgtccca ggtgttgtcg tcggacaggt agaccgcgtc
    28621 ggtccagctg gtggaggtgg gattggtgct gtggttggag accgtccact ccatgtggat
    28681 gttctcgccc gactgcccgt tgccaccaaa gacgatgtcg tccacctgca gatcggacgg
    28741 gggcggcatc tcgaagatgg cgggcgtgct gggggtggca ttgttccgtt ccaggccgcc
    28801 ctcgaagacc ttgccgcgac ccgagccgcg ggccgggtcg gtgacgacga agacatacca
    28861 ggcgccggtc acgtccagcg gcgcgcgcag ggtctggttg accgtgtagc tggcgccctt
    28921 ggcaaggcca cccacatggc gcacagtcgt catgtagcga tcgctgtcca ggtccagcag
    28981 tggatcgcgg gacaggtaga tgtagtcctc ccactcggcc tgctcgggcg gcgtgtcggc
    29041 accgcccagg ttggcgacgg tgtaggtcac ggtgaagtcg ttgcccaccg tgatgtgggc
    29101 ctgcgtcgtc acggtggtga cctgcagatc ggccagcggc gcggcgacca ccggcaaggg
    29161 ggcgacggtg atgttgttgc cctcgccacg gaattccggc accgagtcgc tgttggcgtc
    29221 gatgcccaca ccccggtact cgggcacgat gttgctgttc caccaggaca ggcgggcatc
    29281 cgcctcggag tcggtgaagg ccagcaggct gaagttgccg gagatgccgt cgggcagctt
    29341 gaaggtggct gtgccggtgt agctgtcgcc ggtcttgagg atgccgtagt ggcgcgcttc
    29401 ggccagttgc aggtcgtagg tgtcgaggga gccgtccttg gacaggaaca ggcggtccca
    29461 ccaggcgttc tcgcgggtgt cccggttacc gatgttggtg accgtgtaag tgacggtgat
    29521 ggtcttgccg gactgggcat ccggcggcac caccaggccg gtgaccttca ggtcgggttc
    29581 ccggtaagtg acggggatgt tgccgatgcc gcggttgttc tgctcgccgc ccacgccttc
    29641 gtaggcgctg ccggggtagt agacgtcgcg gaaatacgcg ttgtcgccct gatccgtctc
    29701 gccgttgggg aagcgcgggt cgtaggggtt ggcgtcggtg atgacgtaga ggaagtaatt
    29761 gccaccgatg ccgcggggca gcaccacgtc agcgctctgg gtgtagctgg cgccggccgc
    29821 gagaccgtcc ttgttgctgt ggacaaaggt gcccagggcc gtagcccgct cgggcaggaa
    29881 gaccgggtcg ggcgacaggt agacgctgtc cagccagtag cgcgtgccgg accacacgtc
    29941 gccgccgtag ttcttgacgg tccaggtgac ggtggtcttc tcgcccgaga agttctcgga
    30001 cggcgcggcg atggtgacga ccttcaggtc ggccggcacc gcggtgacgc tggtgtcggt
    30061 gacacgggtg ttgttgtcct cattgccttc gacgacgtcg tagggtttgc cgacatacgg
    30121 gtcgaaatcc gtgtaggcga tgaggtactt gcccgccgcg gccgggttca gtgcgaaggt
    30181 ggccgacgcg gaataactgg cgccgggcgc ggtgccccca tcgtggagga tcgagccgag
    30241 ataccactgc ttggcacccg acgcattgag gtccggcttg tcggacagcc agaagacgtc
    30301 ataccacttg cccgacgcct cgccggcgcc ggcattggtg acggtccagt tgaccgtgag
    30361 atcactgctc gtggacgcct ggggcgacgc cgtgagggcc gtgatcttca ggtcgggcgg
    30421 cggtggttcg aagccgccaa tgatgtcgat ggcccgggct ttgtagttgt tgttgtcgag
    30481 ctcgtgggga tcgtccgggt tgaggttgct ggcgagggtg tcctccagga tcacatcgta
    30541 ggaatcgctc cacacggtga cgtagtaggt gccggaggtg agatgggacg gcaggttgac
    30601 gttggtcacc acgtcgtagc tctcgcccac cttgagggcg ccgttgtgct gcacggtgcc
    30661 gatcatgact gcgctgttgc cgcctgcatt ggggcgggtc ttgtcgcggg acagccagat
    30721 ggtgtcggtc cacgaggcgg cgccggaatt gctcgcggtg gtgccgctgc ccttgttggc
    30781 gacggtgtag cgcacctcga tgttggcccc ttccacggcc tgggccggcg ccaccacgcc
    30841 accggtgacc aggtcggcca gcgggtaggg attgacgtag agcgggaccg cggtgatgtt
    30901 gttggcgtcg ttggggtatt cgtccaccga gccgctgccg tccgcctgga cgatcaggaa
    30961 gacgtcgccg cggaaacgga tcgggatctg caggctgccg ctctcggtgg cgtactgctc
    31021 gcccggattg agggcggcac cgttgtcgta gctgccgatc agcagatcgt cgccggtgag
    31081 cttgttgtcc agcgagaggt agacgttgtc cttccagtgg ggcgtggcgg tgggcacagt
    31141 gccctggttg atgatgctga agcgcaggga cacggccgcc cccgccgacg cgctggacgg
    31201 cgccacgacg gtaccgacct ggagatccgg ccgcggcagc agggccacct gcaggatctg
    31261 atcatcggcg gtggtgttgt tggcggccgc cgtgccgtat tcgtagacgc cgccgctgtt
    31321 ggcgttggca ttggtgacca cgaccgcctg atacacgcct tcggtccgtg ccggcagctt
    31381 gaaacgctcg gtacgggtat atgacttgcc ggcatccagc ccgccagcgt cgaaactgaa
    31441 ggtgcccagg tagatgcccg gatcgttggg ctgacccacc ttgcgcatgt agaccatatc
    31501 ggtccacgac ccgtcggcct tggcggcgcc gttattggtg acggtccact tgacgtcgat
    31561 gtagtcaccc tcgttggcgg tggacggcgt ctggatgtcg gtcacctgca ggtcgggcga
    31621 cggcgacagg gcgatctgca ccggcccggc gacgcgggtg ttgttctggg tgtagacgaa
    31681 ctcgaagggc ccgaaggaac ggacgcgtac gtagtaactg cccgacaggc cgttgggaat
    31741 cgatacgtcc tgggtgcggg tatagctgtc gcccgcagcc agcacaccca ggtgggtgaa
    31801 gctgcccagg ttggccacca cgttgttgcc tgccgcatcg gaggtgagca cgacctcatc
    31861 gttccagccg ggaatgtccg tggggccgat gcccgtgttg gcgactgtcc acgtcacgct
    31921 catggtctgg ccggaactgg ccgtggtcgg cgcgctggcg gcgcttacca ccaggtcggc
    31981 gtagggaatc gacatcacgt cgaagatctg cggcgactcc ttggcgttgt cggcttcgtt
    32041 gccgttctcg aagaccgcgt tcttggcgtc ggtcttgacg aagaagtggt agcggccctg
    32101 gaagtaggcc ggcagcagga aggtttcggt gcggctgtag gaatcgccaa cggccaggcc
    32161 gccggtatgg acgaactcgc ccactacctt gtcgtcgctg ttgccaacga tgctgtcggt
    32221 ggacgcaatg agggcgtcgg tccatgtgga ggtgcggccg acccctgtgc cctggttggc
    32281 gacggtccaa ctcacggtga tctgggccgg gtcaccaatg gtcagggtcg gcgcctggac
    32341 gtcggtggtg acgagctgtg catactggtc cagggtcacg tcgagctgac gcgaagcaac
    32401 gttgttcccc tcggcattgc gctcgggcag ctggttgttg gcatccgccg ccaccagcag
    32461 gtaataggtg ccctgggcgc ccaggggcag atcgaagtcc acgctgccgc catagccctg
    32521 ccccgccgcc agcgggccgg acacggcgcg ggtggccagc agggtgtcgc tggcgtcaac
    32581 ggtggtgtcg cgcgagaggt agatccggtc gatccagtcg ccgcccgccg caccggtccc
    32641 gtcgttgcgg atcgtgtagg tgagtgtcac gcgattgccg gatacggcgg tggacggcgc
    32701 atcgagctgg agcggcacca ggtccggatg cacgagggcc acaggcaccg tggcggcaaa
    32761 ctggttgttt tcgccggcac ttccgctgcc gccttcgtag atctggccat tcacatcggt
    32821 cacgatgacg atgcggtagt tgccgtcagc cagggccggc acgatgaaat cgagggacga
    32881 cgacaggctg ccgccggcgt ccagattgcc ggtgtgggcc acatcaccca ggaaggtggg
    32941 gctgttgagg gtcccgtcgg tgctcaggta aacgcggtcg gtccacggac ccttcgccgt
    33001 gccaccccct ccgttgttga cggtccatgc cacatgggcc gtctgcccgg acacgatctg
    33061 cgcaggcagg gtgatggcgc tcacctggag atcgggcacc ggcgccggcg tgaccttggt
    33121 ggtctgcagc gcggccgtgg tgttgttggt ctcgaagttg gcctcgaaga cccggttttc
    33181 cgcatcggtg cggacgatga agctgtaatt gccggtcagg ccgtacgtca ggaagacgtt
    33241 tgccgacgcc gtgtagctgt cgccaacggc gaggaactgg cccgtatgat ccacctgggc
    33301 cagcaggatg tcgctcgcgt cgagctgatt gtcgctggac aggtagacgt agtccttcca
    33361 ctggctggag tccgtggtgg agtcaccggt gttgcgcacg cgccacgaga ccttcaccga
    33421 atcgccactc tgggccgcag tcggcgccga gacggcctcg acgaccaggt cggcatgggg
    33481 cgcggcgatg ttgatcgcct tggggccgaa tagattgttg gcgcgggtat cgggttccag
    33541 cacctcggcg ttcacgtccg tacgcaggaa cagcttgtaa gcgccatcta cgcgctgggg
    33601 caggacgacc tggcgctcct gggtgtagga ctcgcccggt gcgagactgc caaagtgagg
    33661 cgcggtgacc agcaccacgt cgtcggcgtt gccgtagatg ccatcggcgg acagcacgat
    33721 gcggtcggtc cactgggtcc cggtgggcgc atcgccctgg ttggtgacgg tccaccgcag
    33781 ggtcacggtg tcgcccccac gggcgctggg cggcgcctcc aggctgaccg gggccagatc
    33841 ggcgtatttc ttcttcgcca cgttgaggac aacattggca ctgttgttgg tttcgtcgga
    33901 ttcgaccagc tggccgttgt aatccagact gacggtgacg ttgagcgcgc ccactgcacg
    33961 ctggccttcg ggcagcgtga aggtatacga ccggttgcga tttgcaccgg cggcaatgtt
    34021 gccggcgttg gccacgctcg gatcgtaggt caggcgcgta tccacgaggg tctcgcccgt
    34081 cgcggggttc accacggtga cccgctccac ccagctgccg agggttccgg cgaggccctg
    34141 gttgacggtg tcccattgca ccgtcaccga cgaacccgac tcggtgctgg acggggtggt
    34201 gcgcaggttg cccaccacga gatccggcgc ggaggtgacg gtgagggccg ccgtgttgtt
    34261 gctctcggcc gaatcgttgg cgttgttctc gaagatctgg ccctgggcgt cggccacgac
    34321 gctgaattca aagcggccga tgccctgcgc gtcggccggc cacaccagcg tcaattgccg
    34381 atcgcgaccg tctcccgcgc ccagatcgcc gtttgccgtg gcgtcatagg ccaggctgcc
    34441 cacatagaag gtccggcccg tgctgaggtt acgcaccacg atgctgtcgg tccagccacc
    34501 cgaggtggca cccacgccgg cattcacgtc acgccagctg agcgtgacct gggaccccgg
    34561 cgcccacccg gccgccgggc tgacgttcag gttggccacc tgcaggtcgg cgtagggcga
    34621 aagggtcgag gcgacgctca cggtggccgc gttgttgagt tccgcagcgc cgccggagcc
    34681 gagttccgca acggtgttct ccacgtccgt ggtgacacgg aagctgaggg tgcccgcccc
    34741 gcggttgccg tccggcagcg tcagggtaag gctgtgactc tgcttggcgc cggacgccag
    34801 cgggccatcg accgcttcat cgtggtcgac gagctgggtg tagatcagtt caccgcggct
    34861 gttgcggacg acgatctggt ccttccagct gccgctggtg ggcagggtgc cggcgttgac
    34921 ggtatcccag ctgaggacga cctgaccgcc cgaatgcacg tcgctggaca ccggcgtgac
    34981 cgccaggttg gagacgacga ggtccgcgcc cggctgatcg ccgatggcga tgggcacgac
    35041 cttggagagc ggcgccggag agatgttgaa gctgtaattg gcggtgccga cggcggcggg
    35101 ctgaccttcg acgatcagca tgtaattgcc gctgaaggcc gccgtcgtga catccacgtc
    35161 gacgaagccg ccggggctcg ccacgatgcg cccgtaggga tcgagcagac gccaggacag
    35221 atcgtaggca ttgcggtcca cgccgaggct gtcgaggtag atgcggtcac cggcctgcag
    35281 ggccagcgta tagagggaca tctcggccac cgggctgagg accccggtga ccaccgtgcc
    35341 cggcgtgatg gccgtcgcct gggccacgtc gagcagccgg aactggaagg ccccggtgga
    35401 atcgccgggg gcatcgaaga cgagttgata atcgccggcc ggcaggtcca gccagggctg
    35461 attggtcgtc ccgtcggacg agccgaaggc gcgactgctg accacaacac cgcgcggtcc
    35521 ggtcagggac cacgtgacat tgccgttgta gttgagggca tcgaagaaca cccgcttcct
    35581 gtcacccagc gtgaaggtgt agcggctggt ctggcccggc gcgtcgatgg acgcattgac
    35641 cgtgctgccg agggtcaggg tgtccgtctt gtcggtgacc gtccggagat tgaagctcag
    35701 ggacgccgtc cccgtcgcat tgcggacgcc ctccaccatc agcagatagc tgccgctgga
    35761 cggcagggtg gtcacatccc ggtccgaccc gccccattgc acgtcctgga ccacctcacc
    35821 atgggggccg atgacacgcc aataaggtgt cgaaccggtg ctgcccagca tgtcgaagaa
    35881 cacacgctga ccggcggtgg cgtcgaaacg gtagaaccgg gtgctgtccg ccgggtccac
    35941 gttggcaacc acggtgctgt cgagggtgat ggtggtggcc gccgccgtgg tgtcgaggag
    36001 gcggaacttg taggccccgg tgaagctgcc cgcgccatca acgaccagat cgtaggtgcc
    36061 cgccgccaga tcgagcagca cgccgttgcc ggagcggtcg taggcattgc tctgactgaa
    36121 cgcgcggctg ttcaccacgc tgcccaacag ggaattgctc agcgtccagg cgaccgcgcc
    36181 atcgtttgcc gatccgaggg tatcgaaata gacctgccgc gcctggtcga ggaccagcgt
    36241 gtaatgactg acctgccccg ccgaatcgat gctgccgctg acgacctgac ccagatcgat
    36301 tgtgaccggg gccgggtccg tgatgcgcga gatgcggaag ctgtagttgg ccgtgtccgt
    36361 gtagtagtag ggcccttcga tgagcagggt ataggtgccg gcttccggca gggtgacgtt
    36421 ctccacgtcg taccactggt cggacggccc catgaccacc tgtccggagg gtccgatcag
    36481 gcgccagaag gcgctgttgt agttcccctg cagacgctgg aagtaataac gctcgcccgc
    36541 cgtcgcggtg aagctgtagg catccgtctc gctgcgcggc gaaagctgac cggacacgtc
    36601 cgccttgcca ctgctgtcga gggtgaccgc ggtcgccgcc acaagatcga gcaggcggaa
    36661 ggcaaaggcg ccagcgctgc cgccaaccgt cagagtgtag tgaccggccg ccagatccag
    36721 aatgggcgtg ttgccgtagg agctggcgtt gctgccgtcc gaggaataga aatagcggct
    36781 ggtgaccagc gcgccgctgg gcccggtcag gctccactgg acgtagggcg tgttgctcag
    36841 gacatcgaaa tacagacgcc tggcatcgct cagatccagg gcgtaggtct tgctgtccgt
    36901 cccggaggtc aggttgccat tgacctgcgc gcccagggtg atcgggtcac ccacgggcgg
    36961 cgcaggcagg tccacatggc cttgggacgt gacctggaag cgtgccgtcc cgctgccatc
    37021 atagatgcgg ggttcgatgg caatcagata atgcccggcc ttgggcaggg tcagggtgcc
    37081 gctgtcgttg tagaggtagt tgtagaagag ttgctgtccg tccgtatcga agagccgcca
    37141 gatcgggtag aagctgctcg tgcggtcgag gaagtcgagc atcacctgat cgcccgccgc
    37201 ggcatcgaat gaatagagat gcgaggcgta cggatcgaag ctggtattga cctcggtgcc
    37261 gacggtgatc gcctctgcgg aggccgccgt gagcagccgg aacgcgtagg ccccggtcgt
    37321 gttcccggaa ccgtccaccg tcagggtgta gtcgccagcc cgcagcagca tgaacggatt
    37381 ggtcccgccc aggccatcgg aatccgaata gtagaaattg cggcttacca cggtccccga
    37441 ggcccccgcg agggtccagg taagactgct gttggcggtg agggagtcga aatagacccg
    37501 cgtgtcctgg gccagatgga aggagaactg atcggtctga ccagggatgg cgagcgtgcc
    37561 gttgacggtg ctgtcgaggg tcaggctcgt cttgtcgtcg gtcacccggt acagggtgaa
    37621 gccgtagccg atggggacgg tctgatacgc cgacccctcg atgacgaggg tgtaatcacc
    37681 ggtcgccgcc agactgaaga cgtcgatgtt gctgaaataa ttgctccaca cctgctgacc
    37741 gcggggatcg agcagacgga cgtagggtgt ctgccctaca ttggtgaggg acgtcgcgtt
    37801 gaagaacagg cgatccccag acgtaccggt aaagtgatag accgtgttgg aactgcccgg
    37861 atcgagcgtg ccggacacag gcgtgtcgta ggtcaggttc gagccggcgt tcaggtccat
    37921 cagccggaag gcaaacgggc ccgtggtgtc tgcgtagcca tcgatggtga gcacgtaatc
    37981 gccggcaccg agatccatgg acggcgacgt tccgccgatc tcggcggcat cgctgtagct
    38041 cagggtgcga ttggaaatca ccgtgccgcg cgggcccgtg agcgtccagt agatgttgct
    38101 gttggtggtg aagctgtcca tggccagacg cttggcgctg gccagggtga aggtgtacct
    38161 gacctgttgg ccgggcgtcg tgatcgcgcc ttccaccgca gtcccgctga gactttgcag
    38221 gatgtggcgc acggtcgccg gactgttctg gctggacgtg tccgccacac tgcgtccggc
    38281 gctctcgtca agccggtaat acgccaccag cccggcttca ttgccggaca gggcggtgct
    38341 catgtcggtc gcgatgtcac tggccccgcg caccgtgttc cagatgcgca cgtcgtccag
    38401 gctgccggtg aagcgcgggt agctctcgag ggcgctgccg aacatgagcg gcttgctgtt
    38461 cgacagggcc gatcctcccg cgaggccccc gtcaaccgcc agcgtgccat tgatgtacac
    38521 cttgaggcgg ttgccgatac ggtcgatgac cgccgcgacg tgttcccact gacccagctg
    38581 gacgctgccg acattggtgg acacaccctg ccaggtgccg ttcgtgttgg tggacaggtg
    38641 gagatagccg gcgttattga gccatacggt gacctggcgc tggttcgagt cgccattgcc
    38701 cttgaagacg agaggcgtcc acgtaccgga ataggcgtcg gccttgaacc agaattcggt
    38761 ggtgctggaa cccgtcagat tgagagcatc cgtagcgggc gcctcgaccc agtcgtagcc
    38821 atcgagctgc accgcattgc ccgacttgcc cgggacccag cgcggatcga ggcccgagga
    38881 ctggccgatc accagcggcg tcacactgtc cacgacccgg cgcacattga agctgtacga
    38941 ggcggtcgtc atgtcgccgt tgtagccttc caccagcagc gtataggcgc cggtgaaccc
    39001 caacgtcagg gtgtccacgt cactgagccc acggtagctg aaggcggtgt tgccatacgg
    39061 atccagcagg cgccagcgga tgttgcccgt ggagttgctg agggcatcga aatacgcccg
    39121 atcgccggcg acggcgttga aggtgtaggc cgtggcccct gaggacggcg tcagggtgcc
    39181 gctcacgacc tgatcgtagg tgatcgccgt ggtggctgaa agatccatca agcgcagggc
    39241 gaagccgccg gtcacgtcgc catcgccatc caccaccagc gagtagtcgc cggcatcgag
    39301 agtcacgatc tgctcggtgt cggtgaaggc cgcccgattc accagcaagc cggaaggacc
    39361 gctcagggac cagcggaaat cgctgcggct cggcaacagc gtatccacat agaaggtccg
    39421 cggtccggtc agcgtgaagg tgtaggccgc cgagccccct gcccggtcga tccgcccgac
    39481 gatcgtgccg ggcatcccgg tcagggtatt gaccaccgca ccattgagac cgtgtgcgct
    39541 ggagtcgacg agggtcgagc cccccgaggc ggcgaatcga tagtcggcca ccagcccggc
    39601 cgcgtccggt gccggtgccg tgctgcgatc cgtgtcgatc tgggtctgcg tccggacgcc
    39661 gttccagatg cgcacggtat cgatggcacc gtcgaaattg gcccagccgt tccagttttc
    39721 gttggtggca ccgatcagca gcgggccacc gtgactgaaa gccgcgttgg tgcgcagcga
    39781 cccgctgacg gccagctggc cattgaggta aatgcgcaac tggccgctcg tacggtcgat
    39841 gacccccgca atgtctaccc attcgcccag cttgatggtg ccggagttgg tgctgacgga
    39901 ctgatcgtaa tactggtcac tggaggtgag cagcacgtaa ccgccgctgt tgatccacat
    39961 cgaataggta cggtgattgg agtcgccgtc gtccttgacg atcagcggca tccagccgtt
    40021 ggcgaaactg ttcaccttga cgcgcgcctc gagcgtcagg gagccggtca gattgagcgc
    40081 cgggtcgttg gcacgctgtg cgctgccgta aggccccagc accagcgcgt cgccatcggg
    40141 cgccgcaaca tagaccggat cgaggccagt cgtgccattt acgtcgatgg cctgggacgt
    40201 ggaactgacc ttctccaccg agaaggcgta atcggtggcg ttgccgctgt agtagtacaa
    40261 gccctcgaca atcacggaat agcgcccggt ataggcgagc ttcaccgtgt cgatgtccga
    40321 gaaatagtag cccgaagcaa cctgacggcc gaacggatcg atcagccgga tgtaactgga
    40381 atattgagag gtgcccggct tggtatcgac aaagacctga tcgcccgcct gggcgtcgaa
    40441 gctgaacatc tccatctcgc taccgggcga gagctggctc ttcaccacgt cgccataggc
    40501 aatgaccggc ttgttctctt cgttgaccag tcggaaggca taggccccgg tggcgccccc
    40561 gctgatggtc acaatgtaat caccggcggc gagatcgatg ctggcaggac cgctgtagtc
    40621 ggcggtgtcg ctatacaggc cccgctggct gatgatcgtt ccgcgtggac cggcgaggct
    40681 ccagttgaga ttggtgctgc cgctcaggta atcgaaatag acgcgggtcg actggtcgag
    40741 atggaagctg taacggtcgt tctgggctgt ggtgccgtga ttgccattga tcgtggtatc
    40801 gagcgtgatg cccacgggcg gcaccaccac gggtcgattg acctggaagc tgaaattgag
    40861 ctgaccggag aggctgttgt tgccttccac cagcaacagg tatttgccgg attccggaag
    40921 tgctgacggc gtgaagttat agaaatagta ggacgccacc tgctgccccg agggaccgat
    40981 gaggcgccag gccatgttgt aatagggctg ggagctcgac agggcagaca ggttgagctg
    41041 atcgccggcc tggccgtcaa agctgtacgc agcgatgcca ttggccggat caagcgtacc
    41101 ggagaccatc tgattgacat caatcggctt tgctgcggac acgggcaaga cgctgaatgc
    41161 gaaatcagcc gacgcgcccg acgtggacca gacgtcgatg aagtgggtac cggggttgag
    41221 ggtcagcacc ggcgaattgc cgtaaaggta tccgtcggcg taatagaaat tggtatcgga
    41281 acgctcgacg ccttgcggtc cgctcatgtt ccagtagagc ccgctattgc cggtgcggct
    41341 gtcgaacagc acccgttgcg gctcggtcag ggtgaaacgg tatcgctggt gattgccgga
    41401 actgtcggcc gtcccggtca ccccatcgcc cagctggatg tcattgatca ccgacgattg
    41461 caggttcagc ctgaaatcgt aataggtatc ccaggtctgc cagcggggtc cgctgagcac
    41521 cagcagatag gtcccggtcc gggagaatga aatccccgac tgcgaacccg catatgtcgg
    41581 accaacgacg agattgccgt ccggatcgta gacgaagagt cccccgttcc agctgctatt
    41641 gacgacgaga ttggccacgt cgcccgccgt ggcgtcgatg gaatagacct gcacggcatt
    41701 gcccggctgc agcgtgccgc tcgtctcggt tccgaacgca agcggagccg cggttgaacg
    41761 cgcatccacc aaccggaaat caaaggcctg attggcgggg ccatacagcg tcagggcata
    41821 atccccggcg atgagttggc ccggcggcag gccgtaatag tagtagcccc cgtcactggt
    41881 gtagaacgcc cggtggctga cctcttcgcc ccgcggcccg gtcaaggacc agttgagggc
    41941 gtattcatcc tcgcccagcg cgtcgaacag caggatggtt tcggaattca gggtgaagtt
    42001 atagacgacc ggggttgcga ggtcgtccac ggcgccggtg acctcgtcac ccacattgat
    42061 ggcggtgccg accgggagcg tattgccccc gtcgttcgac aggtagttga ggaacagctc
    42121 ataactgctg tcgttgttgc tgaatacatt cccttcgaca agcagggtgt aacggccggt
    42181 cagttccagc cgggattgct gcggagatcc atccgttgca ccgagcaccc tgcggccata
    42241 gggatcgaac aggctgtaga cagcgccgcc ctcgcccgac agatacgtga aattgaactc
    42301 gacctggtcg ccagactgtc cgtcgaagtt gtagacggtc gtgtcacgtc cgctgtggcc
    42361 gacgtcgacg gtgacggtat ctcccagact gatggcgctg ccggcagacg tgtcgagcag
    42421 gcggaagctg aacggtccgg tgaagtccgc gtaaggatcc accaccagct gatagtcgcc
    42481 cgccgccaga tcgaggaggc cgtcgccgct cgaccagccg tcggaatagg caaaaccccg
    42541 ctgcgccacg agtctgccat ccgcggtata gagcccccac gagaggttgt agtcattgct
    42601 gagggaatcg aacacggcgc gcgtcgcgct ggccaggctg aacgtgaagt gatccacctg
    42661 tccgggggtg tcgatgctgc gatcgatcac gtctcccaat gcaatggctt cggtcacatc
    42721 cgggcactga atcatgcgga acgaaaaatc ggtcgtcccg gatgccgtca catccccttc
    42781 gaagatcagg gtgtaggtgc cgctgtccgc aagcttgggc agctccatga aaccgccgaa
    42841 atagttcaca ccggcaatct gctggccgtc cgcccccagg atccgccaga acaccgtctg
    42901 ggagggcccg cttccggtct gctgcagggc aatccgctca ccggccgtgc cgctgaagct
    42961 gtacatcagg ctgccgttag ccggatccag ggtcgccgcc gtgtccacat cgaaattgac
    43021 tggcgtggcc tgcgtgagat tggccagccg gaaggcgtaa tcgccgctga aatcactgct
    43081 cgcagagaca tacaggctgt agtcgccggg ccccagcgcc agcgcgccag tgctgtaata
    43141 cgcgtcggaa taatagaagc tgcgagggct gaccgtctgc ccgaggggac cgcgcaggct
    43201 ccacgtcagg cgcgaatccg cggtcaggct gtcgaagaaa tactggcccg tctcgccgag
    43261 cgtgaaatcg taacgggcac tctgggccgc agacgccagc gtccctcgcg tcacgtcacc
    43321 gggcgtgagc gtactgacct tctccccggc cttcagcacg cggaacgcgt agggcgttgc
    43381 gtcggccgcc gcaccgatgc gaccttccac cagcagcgta tagcgcccgg ctgccgccag
    43441 gcttccggaa tcgaaatcgc cgaaaccatg gttgctgccg atctgcgcgc caaaaggatc
    43501 gatcagccgc caggtggcgt tgatggtatc gcccgtgttg tcgaagacga atcggtcccc
    43561 cgccgcggca tcaaacgcaa aggcatccgt ctggtagccc gtctccagcg tggcggtgac
    43621 gggcacttca gcgtcgatgg cgcgcgccgc attcaggtcg atcaacctga acgcatagtt
    43681 cccggtcgtg tccatgaccc cgtcaactga aagggtgtag tcacccgcag cgagttgcag
    43741 cacgggctgc gcgtaatccg cgtcggactg cgcgaacgcg cgactgctga ccacatcgcc
    43801 acggggaccc gacaggctcc aggtaaagct gttggagttg gtcagggaat cgaagacgat
    43861 ccgcgcatcg ttgctgaggg tgaatccgta gcggtcggtt tcgccggcca catcgatgga
    43921 accgtcgata cgggtgatcg gcacgctggg tgtgccgtcc agcagaactc gcggctccag
    43981 gggctcgaac gccaaacgat gggatcgggg tgccggttcg tcccggctcg cctggcgcct
    44041 cagcacgggc gcgaggaact gtcccaacca tgcaagcccg cgctcacgcc gcgtcgaacc
    44101 tttggcagag gaagcgccgg tgttttccga acgaaccttg gtatccatga tgatgcaccc
    44161 tatgcaaccg ccgagccatg ccggacggtc cgtattgatc agtatccgga cttcatggat
    44221 gcgatcgatg attgcatccc acgaaatccc ttttcacaaa aaaatcgagg ggcatgaagc
    44281 cccccgatct caattcaatg ttcgatcact cgcacacgca tgatcacgcc ggcccactca
    44341 tccccggcgc atgcggcgga cggccatggc cagcagcgcg gcgccggcaa ggccgaaaac
    44401 gcctggctca ggcagggttc cggtccgttg atccacggtc accagcgggg aaatcgtggt
    44461 cagcgcgttc gtgccgccag cggcgtcata cagcacgccg gaaatatcca cgggtgtcga
    44521 gtcgggaaag gtggcaagaa tgcggaacag caccgagaac aaggggccgg aaccgtagct
    44581 gctcccggag gagttcacca gggaaacgct ctccgaaccg ggcgtgaagc tgcgcagcgg
    44641 cgagaacatc ggatccccgg cgagcacgtc cagcaaggtc agtttggccg tatcgtagcc
    44701 aatggtgaag tcgccaccga agctgatgtc gccgctcagc aggctcacgt tgaggctcac
    44761 ggtggagccg gtgattccgg acgcatccgg cgcaaagatg gcatcggccc gtgccgtcac
    44821 gctcaacagg gagaagacac aggcggcaat caacagcagg actttcttca tgattcagta
    44881 ccaagggaac agtgccttcc actgagcaag ccttgtgcca aattcactaa tactgtctaa
    44941 gcccatgaaa accataaaaa tgtgaatccg cccgcccatc cccaggccaa ctactgtaaa
    45001 aatcgccgac aggcccggca cccggcatgg gccgactgcc ccccaacgcc cttcgggggc
    45061 ttcggaagcc ggacacgtca gaatggaggc acttgcgcag gacggccatc gcacgcagga
    45121 atcgcgcggc gaagtcccat gggcaccgag gcgcggcaca atgatttcaa gcgcctttgt
    45181 cacgtcaaac agaccagtca acgaacaagg ggaaagtgct ttcgcacttt ccccttttga
    45241 ctggagcggc agaagagtct cgaactctcg acctcaacct tggcaaggtt gcgctctacc
    45301 aactgagcta ctgccgcctt gatctaccct ggaggcgcga gccggagtcg aaccgaccta
    45361 cacggatttg caatccggtg cataaccgct ttgctatcgc gccgagaatc aaacggggaa
    45421 gcgtggcttc cccggaaatc ttttggagcg gcagaagagt ctcgaactct cgacctcaac
    45481 cttggcaagg ttgcgctcta ccaactgagc tactgccgct cgacagaccg ccattatagt
    45541 tcaaaaaatc gggctgtcaa acatgtgacg ccccggtgtc agccgctgcg cttcatcagc
    45601 tccggccacg cacagcgcat gtaatacccc atggaccaca gggtgagcac cgccgccaca
    45661 tagatgagca aagtgccaaa ggggcgggtg tcgaagcccc cgagctggtc atcgtagagc
    45721 agcagaggaa tggccgacat ttgcgcagcg gtcttgagct tgccgacaaa ggccaccgcc
    45781 acgctggccg aggcgccaac cttggccatc cactcccgca gggccgagat ggtgatttcc
    45841 cgcccgatga tgatcagcgc gatgagtgaa tccgcgcgtc ccaggtgcac cagcacgatc
    45901 agcgctgccg ccaccatcag cttgtcggcc accggatcga ggaaggcacc aaaggcggag
    45961 gtctgcccca ggctgcgcgc cagatagccg tcgaaccagt cggtgaccgc cgccgcaacg
    46021 aaggtcaggg tggcgaccag gttcttgtcg tccggaccga tcacgtccgc aggaagatag
    46081 tagatgccga cgaagaacgg gatcagggcg atgcgcgccc aggtcaggga attggggatg
    46141 ttcagcggca tgtcggcgtc ggaaggctat cgcagcgcat tgtatatctg ttccgccagc
    46201 tgccggttga tgccgtccac gcggcacaga tcttcaatcg tggcatggcg cacgccgtcg
    46261 atgccgccaa aggccgccag cagcttgcgc cggcgcgtcg gcccgacccc ggcgatgtcc
    46321 tccagccggg acgtgttgcg ggtcttcgcc cgccgcgcgc gatgcccggc gacggcaaag
    46381 cggtgcgcct cgtcgcggac ttcctggatc agatgcaggg ccggcgcgtc gggcggcaga
    46441 tgcaccggcg cacggccgtc cgggaagacc agctcctcca ggcccggctt gcggctctcg
    46501 cctttggcca cgccgacgag gccgatttca gtgaggccca gttcggtgag cacttcgtgg
    46561 gcgacgccca gttgcccctt gccgccgtcg atgaggatca gatccggtcg cgcaccctcg
    46621 cccgccgcca ccttctcgta gcgccgcgtg agggcctggc gcatcgctgc atagtcatcg
    46681 cccggggtga tgccggcgat gttgtagcgg cggtattccg acttcttcat gccggcgccg
    46741 tcccacacca cgcaggacgc caccgtcgct tcgcccaggg tatgggaaat gtcgaagcac
    46801 tcgatgcgct tcggtggatc gcccagagcc agggcgtcac gcagggcgtc gagacgctgt
    46861 tcgccgcggg cggacacctg ttggcgcgcc agcagggcga ggccggcgtt gttctcggcc
    46921 atccccatcc acgcccgctc gttctcgaag cgcggcgcgg caacgggcgc cttgccctcg
    46981 accaccgcct ccagcgcctc gcggagtgtg gcgacgtcgc cattgacgag gatcttgccg
    47041 ggcaagggat gctccagata atgctgctcg acgaaggccg ccagcgcatc cagcggggcg
    47101 cagccctggg cattggccgg gaacagaggc cgatcaccca gatgccggcc gccacggacc
    47161 atggccagat tgacgcacac cagaccgccc tgctccaccg cggcgagcac gtccacgtcc
    47221 tcgtccttgc tgctttccac gaactgcttg tggagcaccg cctgcaggtt gcggatctgg
    47281 tcgcgataga ccattgcctg ttcgaaggcg agacgctccg aggcggcctc catgcgccgg
    47341 gacaggtcgt cgatcacgtc gccgtggcgg ccgttgagga acaggctcgc caactgcacg
    47401 tccgccgcat aggccgccgc gtcgatcttg cccacgcagg gcgcggtgca gcgctggatc
    47461 tggtaaagca ggcagggccg ggagcggttg ctaaaggtgt tgtcctcgca ggtgcgcagg
    47521 cggaagatct tctgcatcag gtgcaggctc tcgcgcaccg cccaggagtt ggggaagggc
    47581 ccgaagtagc gcacgccctt ctggaagccg ccccgatggt agtagatctg gggatactcg
    47641 ccgccagaca gggcgatata gggataggac ttgtcgtccc ggaagaggat gttgtacttg
    47701 ggcccgaggc tcttgatgag gttgttctcg aggatcaggg cctcggcctc ggagcgcacc
    47761 ggcgtcacat ccacccgcgc cacctgggcc accatgaggg cgatgcgcgg gctcagctgg
    47821 gtgcgctgga agtaggacgc gacgcggcgc ttgaggttct tggccttgcc cacatagagc
    47881 acgctctcgt cggcgccgat catgcgatag acgccaggct cctcggtgag ggtgcgcagg
    47941 aaggacttgg cgtcgaagtt cacggatggg ccacgcgggg atcaggccag ggcgagcaca
    48001 gcgtcccgcg cctcgcagta aggtgcccac cgggccagcg tgagcggatc gcccggcaca
    48061 ccccccggcg ccggacgcaa cgcggcgata cgggcgaggc tgtcggcggt cggctgcggc
    48121 tgccaggcgt cgaggagctg caccgccaga tccgggcggt tgcacaccag caccatgtcg
    48181 caaccggcgc gctgggcggc tgcggcacgg gccacgatgt cgccagccac gctcgcccct
    48241 tccatggtca gatcgtcgct gaagatcacg cccgtgaagc caagccggcc gcgcagcaca
    48301 tcctgcagcc agaagggcga gaaaccggcc ggacgcggat cgatgccttc gaagatgacg
    48361 tgggccggca tcacaccgcc cagttcacgg gcgagcacgc ggaagggcac gatgtcctcg
    48421 gcccacaccg cgtcaaaact gcggtggtcc accgggatcg ccacgtggga atccgcctcg
    48481 acggcgccgt gaccggggaa atgcttgccg accgctttca ggccggccgc cgcaagtccc
    48541 tgagccagcg cccgggccag ttccgccgcc acctggggat cgcggtggaa agcccggtcg
    48601 ccgatgacgc cgctcacgcc gtaatccagg tccagcacgg gcgcatagct caggtccacg
    48661 tcatgggcgc gcaactcggc cgccagcacc aggccggcct ggcgggcggc gccgcgcgcg
    48721 tgttccggat cggcatccca gagggcgccg aatcggcgca tggccggcag gcgcgtgaaa
    48781 ccgtcgcgga agcgctgtac ccggccgccc tcgtggtcaa cggagatcac cagccccggc
    48841 cgcagggcgc ggatcgccgc ggtgagggcg cgcagctggg ccgagtccgc gtaatttcgg
    48901 gcaaacagga tcacgccgcc gacgagggga tgctgcagga tgtcccgctc ttcggcggcc
    48961 agttcgaggc cggccacgtc gagcatgacc gggccgggcg ggcgatggga aagcttcatg
    49021 gctgttcgat gacggcgaag gcgagcacgt agtcggcttc atccgaaaga ctcaggtggg
    49081 cgacgaggcc ccgttccgca agataagcgg cgagggccgg ggcgtattcg aagaccggtt
    49141 tgccgagggg atcgtgaccc acgcgcacgg cgtgcagggt ggctggcgcg gcgacgccgg
    49201 tgcccagcgc cttgccgaag gcttccttgg cggcgaagcg tttggcgagg tggcgcgccg
    49261 gctgtcgggc ggcctgccag gcgacgcgct caggctcggc caggatgcgc tcggcaaagc
    49321 gcgcgccgtg acgggccagc gcctcgtcca ggcgggcgac gcggacgatg tcggtgccga
    49381 tcccgtggat caccgggcac cccgctccgc cgcttcgcgc atcaggcgct tcatctcccg
    49441 caccgcctcg cgcaggccgg tgaagaccga acggctgacg atggaatggc cgatgtgcag
    49501 ctcgcgtacg ccgggcaggc gggcgatggg ctggacgttg tagtagttga gggcgtgacc
    49561 ggcgttgaag cgcagaccca gggcgcgaat ggcctcgccg gcgcgggcga tcttgtcgat
    49621 ctcggccacc accgcagggc tctccgcgtc ccggccggcg tcgtggaaga cgtgggcgta
    49681 ggggccggtg tggatctcgc agaccctggc gccgatgcgc gcggcggctt ccacctgggc
    49741 gacttccgcg tcgatgaaca cgctggtgat gatgccctgg tcggcgaggc gggcgacggc
    49801 gtccttcagg cgggcctcct gggaaacgat gtccaggccc ccttcggtgg tcacctcgtg
    49861 gcggccttcc ggcacgaaca tggccagctc gggcttgagg gcgcaggcga tgccgaccat
    49921 ctcgtcggtg gcggccattt ccaggttgag cttgatctgg gtgagctcgc gcagcttgcg
    49981 cacgtccgca tcctggatgt ggcgacggtc ttcccgcagg tgcacggtga tgccatcggc
    50041 gccgcccagg tgggcctcga cggcggccca cacgggatcc ggctcccagg tgcgacgcgc
    50101 ctggcgcagg gtggcgacgt ggtcgatgtt gacaccgagt tcgatcaaga cggttctccg
    50161 ggaaaggcga tgagttcagt gaagacgcgg cgggactgga gcacctgccc gccgaggtaa
    50221 tgctggatga ggcgacgcat cagggccttg ctgccggcga ggatgtccgg atcggaaaaa
    50281 tcgccgcggg ccatgtgcaa caaggtgcgg ccgctgacca gggcggcctc ctcgatgccg
    50341 gcatcgtcga ccggcaccgg gccgcgctcg attatatagg cgtagaaccg gtcgggccgg
    50401 acgggctgcc cgtccacgtc ctcttcgagc ggcaggccgt agcccagctc ctgcaagagg
    50461 gccagctcga agcggcgcaa cacgacctcc tgcgcttcac caccggccaa cgtgcgcagg
    50521 gcctcaccgt aggcgtgatg gagttcggga tggggatctt cccgcggcag caggcgcatc
    50581 agcagttcgt tggcgtaata cgcgacgagc agggcctgcc cgcccagcag cggctgcccg
    50641 ccctgccatt cggcgcgaat cagggtcttc acctcgccgc cgccgctcca gtcgatgagc
    50701 aggggctgaa aggccatcag ggtgccgcgc agggccgacg acggccgccg ggcgcccttg
    50761 gcgaccaggc cgacgcggcc gtggtcgcgg ctgaagacct cgacgatgag gctggtctcc
    50821 cggtacgggt agctgtgcag cacgaagccg ggctgctgat cgacacgctg tttcgccgcc
    50881 atggcagggt gcccgatgga agaccggcgg ccggtttatt cgtagccgag gctcttcagc
    50941 gcccgctcgt cgtcggccca gccgcccttg accttgaccc acacctccag gaagacgcgg
    51001 gagtcgaaga gctgttccat atcgacgcgg gcctcgctgg cgatgcgttt gagacgctcg
    51061 ccgccgcggc cgatcaccat cgccttgtgg ggggccttgt ccacgatgat ggccgcgtgg
    51121 atgcggcgca ggccgggctc ggcctcgaac ttctcgatct cgacggtcat gccgtagggc
    51181 agttcctcgc cgagctgacg gaacagcttc tcccgcagga attcggccgc aaggaagcgc
    51241 tcgctgcgat cggtgatgtc gtcctcaccg aagatgggct cgccttccgg caggtaaggc
    51301 atggcggcct tcaggagctg ggccgtgttc tgaccctttt cggcgctcac cggcacgatc
    51361 tcggtaaagg gatagacgcc gctcatgcgc tccatgaagg gcagcaggcg ggccttgtcg
    51421 gcgagcatgt ccaccttgtt gatgaccagg atcacccgcg caccttcggg cagcagcttg
    51481 accacctgct cgtcctcggg gccgaagcgg ccggattcga taacgaagag caccaggtcc
    51541 acgtccgcca gcacctgggt gacggtgcgg ttcatggccc ggttgagggc attcttgtgg
    51601 cgcgtctgga agcccggcgt atcgacaaag atgaactggg cgccgccttc ggtgaggacc
    51661 ccggtcaccc ggtggcgggt ggtctgggcc ttgcgcgaga cgatgctgat cttctgcccg
    51721 atgagccggt tgagcagcgt ggacttgccc acgttggggc ggccgacgat ggccacaaac
    51781 ccggtgcgga acggggtgtc agcggaaacg gtgtgttcgg tcatgacttg ggcaggcgct
    51841 caagggcccg ctgggcggat tgctgctcgg cggcacggcg gctgacgccc acgccgcggg
    51901 tcttcaggcc gaggccggcg atctcgcact ccacctcgaa ctcctggcga tgggcttcac
    51961 cgcgggtatc cgccagggaa taccggggca gcggcatgcg gcggccctgg agccattcct
    52021 gcagcgcggt cttggggtcc ttgagggcgc tcgccgggtc gatcgccacc agggcatccg
    52081 catagagccg gtcgatcacc gccttggccg tctcgaagcc tgcatccagg aagacggccg
    52141 cgaaaacagc ttcgagggcg tcggccagga tggaagggcg acgatgaccg ccacttttca
    52201 attcaccttc gcccagcttg aggcactcac cgagccgaag ggtctcggcg acccggaaga
    52261 gggcttcctg ccggaccaga ttggcacgca gtcgggacag atccccttcc ctcaacgctg
    52321 caaagcggtc gtacagggca atcgcggtaa cacagttgag gacgctgtca cccaggaatt
    52381 cgaggcgctc gttatggggc gagccgaagc tgcgatgggt cagcgcctgg accagcaact
    52441 ccggccggtt gaagcgatgg ccgagggcca cctgcaacgc atccagcttc atcgggacgc
    52501 tcaacgaccc gtcggtgccg acgtcgcgta gaagtcgatc agcaggctga cgttggacac
    52561 cagcggaatc ttgcgggaat aggacgcggt gatgacgatc ttgccatcct ctttggtgat
    52621 atccagttcg ctcgcaccga tgctggtgat gtcgtcgatg cgcgcccgtt tggtgaacgc
    52681 atcgcgcaac tgggacaccg tggcggtggt tggatccgca ccggaggcca cggcggaaat
    52741 cgcccgccgc acgcccatgt attcgctgac cgccgggacg accttgagga atacgagcag
    52801 cgcggcgccc atcagcacgg cgctcagcgc catgccgatg atgctcatgc ccgactggtt
    52861 ttttcgcgtc atgatcagtg gaaactgccg atgcgtttca gatcgttgaa gttgaaccag
    52921 atgaagaagg ccttgccgac gatattggcg tccggcacaa agccccaccc gcgactgtcg
    52981 aagctgccgt cccggttgtc ccccatcatg aagtaatgcc cctccggcac ggtgcaactc
    53041 acgccgcggc tagtataggt gcagttttcc ctctgcggga actggacaac ctgcgagacg
    53101 aatgaaggta cgtccggttc aatgaggatg ctgtgctcca cgtcgccgag cttctcgatg
    53161 aaccggggcg agaacatccg tgtctgccgg tccgcgccct ttccatcgcc ttggcgggaa
    53221 gggtccaggg catcgggatg caggaaatcc ccgtccggcc tctggggcat ttcaacgcca
    53281 ttgatgacaa gcttcttgtc caggtattcc accttgtcac ccggcagacc gaccacgcgc
    53341 ttgatgtagt cgagggatgg atcggccggg aagcggaaca ccatcacgtc gccccgccgg
    53401 ggctgattga tgtcgacgat cttcttgttg atgaccggca ggcggatgcc gtaggtccac
    53461 ttgttgacga ggatgaagtc gcccaccagc agcgtcggaa gcatggaacc ggacggaatc
    53521 ttgaacggct ccacgacgaa ggagcgcagg cagaacacca ccaggatcac cggaaagaag
    53581 ctggcaccgt attccaccca ccagggcggc ggggcaccgg cgccacgctg cttgcgcgcg
    53641 acgaaatggt cgagtgccca cagcgccccg ctgatcagca acaggacaaa caggatcaaa
    53701 gcaaaattca cgtcgtagtc ccttcacgtc ctaagcagac aggtgtccgc gaaggactca
    53761 tttgccttcg tccacccgga gcaccgccag gaaggcttcc tggggaatct ccacattgcc
    53821 cacctgcttc atccgcttct tgccctcctt ctgcttctcg aggagcttct tcttgcgggt
    53881 gatgtcgccg ccatagcact tggccagcac gttcttgcgc agggccttga tagtctcgcg
    53941 ggcgatgatg tgagagccga tggctgcctg caccgccaca tcgaacatct ggcgcgggat
    54001 cagttcgcgc agcttggcca ccagctcgcg gccgcggtac tgggaggtgc tgcggtggac
    54061 gatgaccgac agggcatcca ccttctcgct cgacaccagc acgtccagct tcaccagatc
    54121 tgccgcgcgg tattccttga actcatagtc cagcgacgcg tatccccgcg acacggactt
    54181 gagcttgtcg aagaaatcca tcaccacttc attcatgggc atgtcgtaga tcaactgcac
    54241 ctggcggccg tgataacgca tgtctacctg catcccccgc ttctggttac acagggtgat
    54301 caccgggccc acgtagtcct ggggcaggaa gatggtggag gtgatgatgg gctcgcggat
    54361 ctccgccatc ttggacgctt ccggcaggcg cgacgggttg gagacctgct ccacgctacc
    54421 gtcgttgagc acgacctcat agaccacggt gggcgccgtg gtgatgagat ccatatcgaa
    54481 ctcccgctcc aggcgttcct gcacgatctc catgtggagc aggccgagaa agccgcagcg
    54541 gaaaccgaag cccagcgcct gggagacttc cggctcgaac tgcagcgacg cgtcgttgag
    54601 gcgcagcttt tcaagggatt cccgcaactg gtcgtattcg ttggattcca ccggatacag
    54661 gcccgcgaac acctggggct tgatctcctt gaagcccgcc aggggctcgg ccgcaggctt
    54721 gttggccagg gtgatggtgt cgcccacctt ggcgaccttg agttccttga tgccggcgat
    54781 cacgaatccc acctcaccgg cagacagggt ttcccgcgcc acactcttgg gcgtgaacac
    54841 gcccacctgc tcgcacagct gctgcacgcc ggtggccatg aactggatct tgtccttggg
    54901 acgcaggccc ccttccacga cgcggaccaa catcaccacg cccacgtagt tgtcgaacca
    54961 ggagtcgatg atcagcgcct tgagcggcgc ttccggatcg cccttgggcg gcggcacccg
    55021 cgcgatgatg gcttcgagga tgtcctcgac acccaggccg gtcttggcgg agcacggaat
    55081 cgcatcggtg gcgtcgatgc cgatgacgtc ctcgatctcc tgcttcgcgc cgtccgggtt
    55141 ggcctggggc aggtccatct tgttgaggac cggcaccacc tcgacgccct ggtcgagggc
    55201 cgtatagcag ttggccaccg tctgggcctc gacgccctgg gacgcgtcca ccaccagcaa
    55261 cgccccttcg caggccgcca gggagcggga gacttcgtag gagaagtcca cgtgtcccgg
    55321 cgtgtcgatg aggttgaggt tatagacctg cccgtcccgc gcgcgatagc tcagcgccgc
    55381 cgtctgggcc ttgatggtga tgccccgctc ccgctcgata tccatggaat cgagcacctg
    55441 ggcttccatt tcccggtcgg acaggccgcc gcagcgctgg atgatgcgat ccgccagcgt
    55501 ggatttgccg tggtcgatgt gggcgatgat ggagaaattg cgaatgtgtt gcatggagca
    55561 ataaaaaggg tgccgacggg cacccgaggg aaatgtgttg gccttccaag ggcttggatt
    55621 ctaaccgatc cgccggaaat gagcacggat cgccgccgcg tccaggaagt gacgacacag
    55681 ctccccctcc cccgccaaca cgaccggcac cgcttcgttc cagcgctcct cgagttccgg
    55741 atcgctgtct acgtcaatga cctcgaccga gaagcccagt tcgacctgca gcggcgccag
    55801 cgcgtcgagc aggtcgtggc acaggtggca ccacgcccga ctcaacacgg tgagatggcg
    55861 gtcagcgctc aagggtgcgc aggggtacaa aggtctgcac gtcgcctcgc tggaccaggg
    55921 ccgacaccgc ctgcccgccc tcgaaggctg ccacgacccg gttgaactgg tccgctgaac
    55981 ggatatccac ccgcgtgccg cgactcacca gcgcgagaat cgcatcgccg gcctggatct
    56041 cggacgcccg cgccgaaggg ctgcgcaggg catccacgac cacgccggca ccgatcttgc
    56101 ggcgctgttc cgcattcggg tccaccacca ggatgccgag tttgttgggt ggggcttcgg
    56161 gccgcatgac cttggcaatg actttttgcg gcttttcgtc gggcaactcg gcgaccgtca
    56221 ccgtcagatc cttctgtacc ccacgccgcc acacctgggc caccaccctg ctgcccggac
    56281 gggtgccgcc caccaggcgc ggcagctcgc tcgaaagccc cacagaccga ccgtcgaatc
    56341 gcagcacgat atcgccctgc tcgaagcccg ccttgtcggc aggactgccc ttctcaaccg
    56401 aactcaccag ggctccggcc ggacgggcga gattgaagct ctcgcccaga tcccgcgaaa
    56461 cctcctggat caccacgccg atacgtccgc gctgcacgcg accgttctga cgcagttgtg
    56521 cctgcacatc catggccacg tcgatgggaa tggcgaagga caaacccatg aacccacccg
    56581 tacggctgta gatctgggaa ttgatcccga ccacttcacc cttcatgttg aacagcggcc
    56641 caccggaatt gcccggattg atggccacat ccgtctggat gaagggaacg aaattttcct
    56701 gcggcagggc gcgccccttg gcgctgacga tccccgccgt caccgaattc tcgaacccga
    56761 agggcgcacc gatcgccacc acccagtcac cgacgcgcag cctggctgga tcgccgaacg
    56821 ccacccgcgg caggccggac gcctcgatct tgagcagggc aatgtcggag cgtgcgtcgg
    56881 cgccgatcag acgggcgcgg aattcgcgct tgtcggtgag cttgacgacg atctcgtcgg
    56941 cgctgtccac cacgtgggcg ttggtgagca cgtacccatc ggacgacacg atgaatccgg
    57001 accccagcga acggtcgtcc gggtccatcc gcggtacgcc acccgggcct cgcggcacga
    57061 aacgacggaa gaagtcgaac atgggatcgt cttcgtccag attcggatgg gctccgcgga
    57121 caccggaacg acctgcctgg gtggtgctga tgttcaccac cgctgcgccc tgacgttcgg
    57181 caagctcggc cacgtccggc agatcacgtg cccacgccgg acgcaccagc atcaaggccc
    57241 ccagcagaac catcacccag atgaatcgcc ccagggtgcg ctgcatcatg cgccgcaccc
    57301 cgtaccgtcc gcatcccgca ccaggcgcac gggcgaggtc cgctcgcccc agcgcccacg
    57361 accggcaaca aagccacccg ccagccccag gacagcgccc accaccgccc ccaggtcgcc
    57421 ccgctccggc gtcgcaagca cggtcccgag cgccgcgccg gcgatgacgc ccagcaccgg
    57481 cagaccgtag gtcagcaacg ccgcccgcag ggccactccg tcctcgatca ccacgccgac
    57541 ccgctcgcct ggcacacatc ccggtacatt gggcagccga taggtcttgc gggacgtttc
    57601 cagcgcccgt gtgatgctca cccctccgca gccacccggt tcgtgacagc gtccacagcc
    57661 ccctgcaggc gtcacctcaa cccacgcgaa gccttgttca accttctgca caactccggg
    57721 aacccgcatc atttcttccg taattcgatg ccatccgcca gacgcaccag cgccgcagcc
    57781 ggcacttcgc ccagcaccgt gatcagatag tcacccgcac tccgccgcag gatattgaac
    57841 gacccggtgg cactcatacc actctgaatc accgaactct tgcctgcatg aggctcgata
    57901 aagaccgata tggccgacag gccatccgaa aacaccacgt gatacgcctc cggtcggtcc
    57961 tgccggatct ggcgcaagac ggacacgacc tgacgatatc ccgggagcgc gttgcggaag
    58021 acccatcctg catcctccat gcgcacttcc gttccgcgcg cattgacgac cttccagtcc
    58081 gggtcacgga cgatgtgggc ccgcaccttg tctcggtcga ggggcacccc gatctgcact
    58141 tcggaaaagg caaactgctc gatgctgtca cccttttcgt cgatcaagcg ggcccgcagc
    58201 aacaggccgg aattcacgtc cgcccagaac tgatgcccgt aacgcaggtt gtccttcggt
    58261 tcgaggatga tctgctgact ttcaagtcca gcgatacgta ccatttcgcc cttgcggatc
    58321 cggtagtttt ccgcaagcgt gttgaccgaa ggcggcagac gcgcaggaaa gctgcgccgc
    58381 accgagggct gatcgacgat cagcatgcgc tgatccggca ggtagcactg gacctgatcg
    58441 ttgctacgaa ccacttcccg cggactgccg tcaagcgctt cgagccgctc gaactcgccg
    58501 ctgccatcca ccaggtgggc aatccgggag gtctcgaccg tcttgccgtg ctgataggtg
    58561 aacgtcccgg aataattcaa ccgatgggcg gcaccggcaa tacgcccgag ccagctcagg
    58621 gggtcaccgg gcgcctcggc gcgggcagtt ccgcaacagg ccagcaggac cagcaggcaa
    58681 tgccggaagc gactcatcgc tgaccgggca tggcggtgtc cgccaccgtc ctgacataca
    58741 gcgcgacgcc cggcatcgcc gccgagggcg ccagggactg gtgagcgaag aggtattccc
    58801 ggttcgaatt ctcgacggag gtcggcatca ccatcgactg tccggacgtc gcaggcgtca
    58861 cactggccat cagcgcggga gatttgttca ccttggcaaa gtgcagcgcg tcaccgccac
    58921 cgctgaacat gccggcaacc cagcctaccg ccgccacccc catcagcgag gcagcgagag
    58981 gcatgagatg ccggaagaag ccgctccctt cccgccgaac cgccggtgcg agtacggtgg
    59041 gctcgtcttc gagacgcagc atcacactgc gagtgacgtc cagggccagc ggttcatcgc
    59101 cccgcagggc atcgcccacc aggcaaaaat gctcccagtc ccgtcgcaat gccccatccc
    59161 gcttcagatg atcgagcatg cgcaccgtcg attcctcgtc cagcgcaccg tcgagcaggg
    59221 cagaaacacg ttctttcatc ttctcaccac ctcttgttcg gagccgtgtc gagcaacggc
    59281 ttgagctttt cggaaatggc ctcgcgtgct cggaagatgc gcgaccgcac ggtgccgatg
    59341 ggacacccca tgatcgtcgc aatctcttcg tagctcagcc cttcgatctc ccgcaacacg
    59401 atggccgccc tcagttcctc cggcagggcc tccatcgcaa ggtttaccgt ctccccgatc
    59461 tgcttggtca tcaacagccg ctcgggtgta tccacgtcgc gcaactgatc gccgtcctcg
    59521 aaggtttccg cttcctccga atcgaattca gtcgtcgtgg gtgccctgcg cccctgcgac
    59581 acgaggaagt tcttggcggt gttgatcccg atgcggtaca gccaggtgta aaaagcactc
    59641 tccccccgaa aggacggaag ggcccgatag gccttgatga acgtctcctg ggcgacgtcc
    59701 tccacttcgg cagggtctcg gatcagacgg gacagcaggc gggcgagctt acgctggtac
    59761 ttcgccacaa gcaggccgaa cgcctgcttg tcgccgcgct gtacacgctc gaccaactgc
    59821 tggtcaattt cgcgatcgct catggtcgga atactggaat cggaacatcg caacccaaaa
    59881 cggtcgcagg acccccagta taccgtgagc ctccaaatct ccgccaaagc actcgctaga
    59941 cccgccgcca cggcgtaagt tcagtccgac cccgccctga gcaggggcaa tttcacgtgc
    60001 tatagtcgcc ccttcgatct cgagccctcc gaggcacccc cagaagtgag cgttttcgac
    60061 gtactgatcc tcggcagcgg tcttgccggc caatccctgg cgctgcgcct cgccgacacc
    60121 cacaaggtcg ccctcgtcac caaacggcat ctcgaagaca gcgccagcgc ctgggcacaa
    60181 ggcggcatcg ctgccgtgct cgatcccgcg gacagcattg agtcccacct gaccgacacc
    60241 tttgtggccg gcgccggctt gtgcaaccca ggctcctcgc gcttcgtcgt cgaaaacggc
    60301 gcccgtgcca tccagtggct gatcgagcgg ggtgtgccct tcaccgcgga tgcccagtca
    60361 cccattggct accacctcac ccgcgaaggc gggcatggtc agcgccgcgt gatccacgtg
    60421 gccgacgcca ccggtgccgc cgtacaggcc acgctcaccg agcaggtccg ggcccatccc
    60481 aacatcgcca tcttcgaaca gcacatcacc gtcgacctga tcgtcgggtc caagatcggg
    60541 cgtctggagg aaggctgcct cggcgcctac gtgctggata tcgcgcacga cgaggtacgc
    60601 accttcgcgg ccggccacac ggtgctggcg acggggggtg cgggcaaggt ctatctgtac
    60661 accaccaatc ccgacacggc cacgggcgac gggatcgcca tggcctggcg ggccggctgc
    60721 cgggtggcga acatggagtt catccagttc caccccacct gcctctacca cccgcaggcc
    60781 aagtccttcc tgatttccga agccgtgcgt ggcgagggcg gcctgctgcg gcgcccggac
    60841 ggcagccgct tcatgcccga gcacgatacc cgcgaagagc tcgcgccccg tgacgtggtg
    60901 gcccgcgcca tcgacttcga gatgaagaag cacggcctgg actgcgtctt cctcgacatc
    60961 agccacaagc cggccgaatt catcgcggaa cacttcccca acatccacgc acgctgcctc
    61021 gaactgggca tcgacatcac ccagcaaccg atcccggtgg tgcccgccgc ccactacacc
    61081 tgcggcggcg tcgtcaccga cctgcatgcc cgcacggacg tccccggtct ctacgcgatt
    61141 ggcgagaccg cctgcaccgg cctgcacggt gccaatcggc tggccagcaa ttccctgctg
    61201 gaatgcctgg tatttgccga ggccgccgcc caggacatcc gcagccatct tcgccacgcc
    61261 cccgcggatc taccgacatg ggacgagagc cgcgtcaccg acgctgacga ggccatcgtg
    61321 atctcccaca actgggacga actgcgccgc ttcatgtggg attacgtcgg gattgtccgc
    61381 accaccaagc ggctgcagcg cgctcagcac cggatccgtc tgctggcccg ggagatcgac
    61441 gagttctaca gcaacttccg ggtgaccaac gacctcatcg aactgcgcaa cctggtggtg
    61501 accgccgatc tgatcgttcg cagcgccctg cgccgcaagg aaagccgcgg cctgcacttc
    61561 agccgtgatt atccgcagct ccagcccaag gcacgggaga ctgtcctccg tccaccacgc
    61621 cgcagctgaa ggcgcggacg cgcaggatca ttcaggcgac gtccgagtcc ggctccgcaa
    61681 acgtcttggt cacgcgcaga cgcaaccata cctgcaaggc gcgccagtcc tgcgtggaca
    61741 tctggtccgg caacagccag cgggcgccac gtcgcccatc gtcgtcccag atcagccaga
    61801 tcgaacgatg aagcgcgacg gactccgcgc gcacgcgtcc ggcctgtgcc gccattccgc
    61861 tgaactgcca cgccagacgt ccgtccgcat gcagttccag gcgaccggac tgccccagcg
    61921 cctgcttcac ttcccgataa agcgatgctg cgatcaccag cccgaccggt gcggtgatcc
    61981 acggcgatgc caggccggcg acacatgaaa aagccgccgc ggcgtgcagt gtcaccagca
    62041 agatgacgcg cagccgcgac ggcccgaatt caagcacatt gatcacggag ccgaaccccc
    62101 gggaccacgc acccggtcaa acccggcgga acaccagcga accgttggtt ccgccaaagc
    62161 cgaaattatt cttcagcgcc acatcgatct tcatgggacg ggcgacattg gcacagtaat
    62221 ccagatcgca ctccggatcc tgctcgaaga tgttgatcgt cggcggcgaa acctggtgat
    62281 gaaccgccag ggcagtcagt acggactcaa gaccacccgc gccaccaagc aggtgacccg
    62341 tcatggactt ggtggaattg accaccagcg tcttagccac ctccacgccg aaggcctgct
    62401 tgatggcctc ggtctcattc ttgtcaccca gcggcgtcga ggtcccgtgg gcgttgagat
    62461 actgcacctg atcaggattc acgccggcgt cccgcagcgc gttgagcatg ctccgacggg
    62521 ggccgtcggt gtccggtgcg gtcatgtgga aggcatcacc gctcatcccg aagccggcga
    62581 gctccgcata gatgcgggcg ccgcgcttca cggcatgctc gtattcctcg agcaccatca
    62641 caccggcgcc ttctcccagc acgaatccgt cccggtcctt gtcccacggc cggctcgccg
    62701 tcgcaggatc gtcgttgcgg gtggacaggg ccttcgctga cgcgaagccg cccatcgcca
    62761 gggggcacac cgtcgactcg gcaccgccgc agaccatcac atccgcatcc ccatactgga
    62821 tcatgcgcat gctgctgcca atggcgtgca gcccggtggt acaggccgtc accaccgcca
    62881 ggttcggtcc cttcagaccg aacatgatgg acaggttgcc agagatcatg ttgatgatcg
    62941 tgcccggaat gaagaacggc gagatcttgc gcgggccacc agcgacatag tcggtgtgcg
    63001 tcccttcgat catcggcagc ccgccgatgc cggagccgat gttcaccccg atgcgatctg
    63061 cattggcgtc ggtgacttcg attccggaat cgcgaattgc ctgaatcccc gcagcgagcc
    63121 cgaaatggat gaaagtgtcc atccggcgcg cttccttggc ggaaatgtac gcgccgacat
    63181 cgaacccctt gacctccccg gcgatccggg ccgacagggc ggatgcgtcg aatcgggtga
    63241 cggggccgat cccggaacgg ccgttgagga tgctgtccca ggcttcggac acggtgttac
    63301 cgaccgggga gatgactccc agcccggtga tgacgactct gcgagcggcc aagacgaact
    63361 ccggatcgaa ggaattactt gttcaggtga gcggacacgt aatcgatggc ctgctgcacg
    63421 gtggtgatct tttcggcttc ctcgtcgggg atctcgcact cgaattcctc ttcgagggcc
    63481 atcaccagct cgacggtgtc gagagagtcg gcacccagat catccacgaa ggaggattcg
    63541 ttcttcactt cggcttcgtt gacgcccagt tgctcggcaa caatcttctt gacgcgctgt
    63601 tcgatatcgg acatgtgtaa ctccttccct gattgtgcgg gtgaattcaa aaacccccgc
    63661 attctgccaa aaaatgcgca ccctcgccac cgcggcgagg atcacgccat gtacatgccg
    63721 ccattcacgt gcagggtggc ccccgtcacg taggcagcgg cgggcgaggc gaggaagccc
    63781 acgaccgccg cgatttcttc gggcttgccc agacgaccga gggcgatctt cgtctgcagc
    63841 gcctttttct gatcttcggg cagttccttg gtcatgtcgg tgtcgatgaa ccccggcgcc
    63901 acgcaattga ccgtgatgtt gcgactgccc agttcctggg cgagggaacg ggtcatgcct
    63961 gctacgccgg ccttggccgc cgcatagttg gcctggccgg gattgcccga gctgcccacc
    64021 accgaggtga tgttgatgat gcgaccgtta cgggccttca tcatgccctt ggtgaccagc
    64081 cgcgacatgc gaaacaccgc cttcaggttg gtgtcgatca ccgcgtccca ttccgtgtcc
    64141 ttcatgcgca tggacaggtt gtcgcgggtg atgccggcgt tgttcaccag caccagcacc
    64201 ggaccgaagg ccttttcgat ctcggccagc gccgcttcgc aggccgcggc gtcggtgacg
    64261 ttcaacatca tgccgcggcc gctgacccca gccgcgtcga gcccggcctg gatgtcggca
    64321 gcgcccgctt cggaggtcgc cgtgccgatc accgtcgcgc cgaggcgccc cagttcgagg
    64381 gcgatcgccc gtccaatgcc ccgcgaggcg ccggtcacca gcgccacctg tccctgcaaa
    64441 acctgactca tgccgccccc gtcgtctgga gaatctcgtc aaggctggcc gcgtcggcga
    64501 tcgcgccgcc ggtgaggctg ccctcaatac gcttggtcat gcccgccagc accttgccgg
    64561 gaccgcactc gtagacgtgc gtcaggccgc gccccgcaat cgcctggacg gactcgatcc
    64621 agcgcaccgg cgaataggcc tggcgcacca acgcgtcctt gatgcgggcc ggatcggtct
    64681 cgaccgccac gtccacgttg ttgagcaccg gaatggccgg cgccgccacg acgatatcgg
    64741 ccagcgccac ggcgagccgc tcggccgccg gcttcatcag tgcgcagtgg aagggcgcgc
    64801 tcaccggcag cagcacgccg cgcttggcgc ccttggtctt ggcgaccgca atggcgcgct
    64861 ccaccgcggc acgggcaccg gcgatgacga tctgccccgg cgagttgagg ttggccggcg
    64921 agacgacttc accctgggcc gcttcggcgc aggcttcggc cgcagcaggc tgatcgaggc
    64981 ccatcagcgc cgccatggca ccttcgccgg ccggcacggc ttcctgcatg gcctgggcgc
    65041 gcagacgcac cagcttgacc gcgtcctgaa agcccagtgc accggccgcc accagcgccg
    65101 agtactcgcc caggctgtga ccggccacca gcgccggcgt acggccaccg cgggcgatcc
    65161 attcgcgata gacggcaatg ccggccgtca gcatcaacgg ctgggtgttg acggtcagcg
    65221 ccaaggtttc ggcggggcca tccgccacca tctgccagag gtcttcaccg agcgctgcgg
    65281 aggcttcggc gaaggtgtca cggatcaccg cggaatcgcc gtaggcggcc atcatcccga
    65341 cggactggga cccctgacca ggaaaaacaa acgcgaaatt cattgttgtg gacccttcga
    65401 tgccgtatcg agaactcagt attcgaggag cagcgcgccc cacgcgaagc cgccgccgat
    65461 gccttccagg atcaccttct ggccgcgccg gatgcgcccg tcgcgaaccg ccgcatcgag
    65521 ggccagcgga atcgaggccg ccgaagtgtt gccgtgctcg cccacggtgg cgatcacccg
    65581 ctccggcgcg acgcccaggc gcttgccggt ggcctggatg atgcggatgt tggcctgatg
    65641 cggaatcagc caatccaccg cgtcgagggg cacgcccccc agctcggcca cttccttggc
    65701 cacgtcggac aacaccttga cagcaaactt gaacactgcc tgaccatcca tccgcaggaa
    65761 aggatcgccg gtcacctgac cccggctgat ctgccccggc acgcacagga tctgattctg
    65821 cgctccatcg gcgtgcagcg cggtggccag gatccccggt cgctcggacg cttcgagcac
    65881 caccgcaccg gcaccgtcac cgaagagcac gcaggtgccg cgatcgttcc aatcgaggat
    65941 gcgcgagaag acctcggcac cgaccaccag ggcacgcttg tgcgaacccg aacggatgaa
    66001 cttttcggcc gtggcgatgg cgaaggcgaa accggcacag acagcctgga catcaaacgc
    66061 cggcgcaccg ttgctcatgc ccagccgggc ctgcaggagc gtggcagtac tcggaaagat
    66121 gtaatcaggc gtggaggtgg cgacgatcac cagatcgatg tcgttgggcg tacaggcggc
    66181 cgcctccagg gcccgcaagc tggcctcctg ggccaactgg ctggaggtga cttcgggcga
    66241 agccaggtgg cggctgcgga tgccggtgcg ctcctggatc cacgcatcgg atgtgtcgat
    66301 gccgcgggcc accaggtcgt cgttggtcac cggcgcgccg ggcagatagc tgcccgtgcc
    66361 ggccactcgt gcatagatca tgccgcgggc ctctcccgtt ccatgcgtgc gctgatgcgc
    66421 tcgatcagat tgttgcgcgc cgcttccact gcacgccgga tagcctgctc aaaggcaaag
    66481 acgtcggcgg cgccatggct cttcaccacc actccacgca gacccagaag ggccgcgccg
    66541 ttgtagcggc gcgaatccac cttgtgcttg aagcccttga gggccgacat ggcgaagacg
    66601 gccgccagct tggtcaccca gttgcgggag aactgctcgc gcaggaaggt ggccagcatc
    66661 tgggccaggc cttcggaggt cttgagggcc acgttgccga cgaaaccgtc gcagaccact
    66721 acatccacgg tgcccttgta gatgtcgttg ccttccacgt tgccatagaa attgagtccg
    66781 ctatggcgca acagctcgcc ggcctgcttg accgtctcgt tacccttgat gtcttcctca
    66841 ccgatattga ggagaccgac gctcggacgc tcattgtgct cgaccgcagc caccagcatg
    66901 gcacccatga tgccgaactg gagcagatgc gcgggcgagc aatccacatt ggcacccagg
    66961 tcgaggacat gcgtctgccc acgcagggtc ggcagcgtgc tgcagatcgc cggccggtcg
    67021 atacccggca gcgtcttcag cacgaagcgg gagatggcca tcagcgcgcc agtattgccg
    67081 gccgacacgg ccgcctgggc gtctccggat ttgaccagat ccaccgccac gcgcatggac
    67141 gagtcctttt tgttgcgcat cgcgagggcc ggcggctcgt ccatgccgac cacttcggtg
    67201 gcatggcgga tgctcaggcg ctcgccgtag cgcacctggg ccacaccgag ttgctgttct
    67261 acggcttcgg cacgccccac cagcaccacc cggacatccg gatcggtatc gagaaagcgc
    67321 aaggccgccg gaacggtgac ggaggggccg tgatcgcccc ccatgcagtc gatcgccagg
    67381 gtgactgtca ttcgctgtcg ggcaagaggt tcactcgcaa aaacggcgcg ttcacccagg
    67441 ggtgccgcgc cgctttcgga ccggggagat tattcgccct tggccttggc aaccttcttg
    67501 ccacgataga cgccggaagg cgacacgtgg tggcgcagat gcacctcacc ggtggtcggc
    67561 tcgacggcca gcgggggagc caccaggaaa tcgtgggaac ggtgcatacc gcgcttggac
    67621 ggggacttct tgttctgttg gacagccatg aaacactcct tgaggaatac ttaagccttg
    67681 ccgcgcagat tccgcaacac ggcgaagggg gaaatctctt catcgccgcc ggctttcacc
    67741 ggcgcttcgc aagcctcgtg acggggcacc agcggcagcg cgaggagcag ttcatcctcc
    67801 accagcgcca gcacgtcggt ccgcggctcg acctccagcg catccacctc atcgttttcc
    67861 agatcgtctt ccggaatctc ctcgccaggg cgcaggagca gcagacggtt atggatcacg
    67921 cagggcaggc gcagcgcatc gagacaacgc tgacactgca gcgtcaactc agtccggatc
    67981 atcagatcgg cgaacagctt gccatcgtcg tcccgcaccc cgtcaaagcg aaaccacact
    68041 gaacccaccg gatcgctcac cgaatcggcg agtcgttcga gccgggccac cggaatttca
    68101 ccctcgacgt gccggccatc cctggcgagc gcccgaagat cgatcacgaa gccttcttgc
    68161 gacataagcg cggaatgata ttatttcggg ctgcccgtgt caaaacccag ttcgcacggt
    68221 gtgcctcgat gcgttcacgt tccggcactc cgggccccgc cccgatgaaa ctcgtcctcg
    68281 cctccacctc ggcctaccgc cgggaactcc tgctgcgctt cggcctgccc ttcgacgtgg
    68341 cccgaccgga catcgacgaa tcgccgctgc ccgacgagag cccccgcgcc accgccgagc
    68401 ggctcgccgt cggcaaggcc cgtgcggtcg cggcacagtt tcccgatgca ctgatcatcg
    68461 gcagcgacca ggtggcggcc ctcggcgaac accgtttcgg caagccgggc tgcgtcgaac
    68521 gcgccgtcgc gcaactcaag accatgagcg ggcagacggt cgtcttccac accgccctgg
    68581 cgctgctcaa cacccgcaat ggcgccgtgc aggtggatgt ggtgccgacc gaggtgcgct
    68641 tccgcgccct gagcgaggac gagatcgtcc gctacgtcga gcgtgaacgt cccctcgact
    68701 gcgcaggcag cgccaagtcc gagggcctcg gcatcacctt gctggacgcg ctcaccggtg
    68761 acgaccccaa cgccctggtg ggcctgccgc tcatcgccct cgcccgcatg ctgcgtgccg
    68821 aaggcatcgc cctgccctga tggccggcac gctgtatctc gtgccggtga gcctggggcc
    68881 cgcccccacc gccctcacca cacccgccgg cgtggccgcg caagcctgcg ccctcgacta
    68941 ttttgtggtc gagaacgcca agtccgcccg cgccgaactc aagcgcctgg agcacccccg
    69001 cccgctgcgc gagctggaca tccgcgagct gcccgacaag cccaacccgg ccgatctgga
    69061 cgcgctcctc gcccccattc tcgccggcac caacggcggc ctgatgtcgg aagcgggctg
    69121 cccggctgtc gccgacccgg gggccctgct ggtgcgccgc gcccatgaag ccggcgtgcg
    69181 cgtggtaccg ctggtggggc cgtcgtccct gctgctggcc ctcatggcct cgggcctcaa
    69241 cggccagagc ttcgccttcc acggctacct tccggtggac gaggacgccc gcaaccgacg
    69301 catccgcgaa ctggaagccg aatccggccg ccagcagcgc acccagctgt tcatcgagac
    69361 gccctatcgc aacgaacgca tgttcgaggc cctgcgcacc gtctgcgcac ctaccacgcg
    69421 cctgtgcgtg gcccgcgacc tgagcctcga cagcgaatgg atcgccaccc gcacggtggc
    69481 ggcctggaga aagagcgccg cgccagaact ggcccggcac ccgaccgtct ttctgatcct
    69541 ggccggctga gcactcaggc gcccagtacg cccttcagaa acacgccgcc gtcccgctcc
    69601 tccaccgcca ccggaaccag gcgacggccc cggcacggac cggccacgca gtagccggat
    69661 tccggttcgt acagcgcccc gtgggtggcg cagatcagat acgtcccgcc agtatcgaag
    69721 aatctccccg gttgccagtc cagttccacc ggcacatggg cgcaactgtt gcggtaggca
    69781 tacacccgtc cgccgtagcg caccaaaaag gctgcaacgg cctcaccaca cacctgaacc
    69841 gtgaagcgga tgccgtcgcc cccttccagg acctcgtcag accgacagat cacgcgtgcc
    69901 gctccagcca gtcgcgcaag gccaccggtg aatggaggca ggccagcggc gcctctgcct
    69961 cgagctgttc gaccggatgg gcgccgaagc tgaccgccag cgccgccacg ccggcattgc
    70021 gcgccatctg cagatcgtgg gtcgtgtcgc cgatcatcag gcaggcgtcg ggcatcacgc
    70081 ccagctcgtc cagcagctct tccagcatct gcggatgggg cttggagaag cactcgtctg
    70141 cgcagcgggt ggcgtgaaag aagcgaccga gacccgagaa ccccagcgcc cgttccagac
    70201 ccttgcggct cttgccggtc gccaccgcca gcagatggcc gcgcccatga agggcttcca
    70261 ccatgtcaaa ggtgccgtca aagagggtca gttcgtgatc gccggacagg taatggtggc
    70321 gatagcgctc caccatgcgc gggtagtcgt gttcggccag gtccggcacc gcatggcgca
    70381 gggcgtccac cagccccagt ccgatcacat gacgggcccg cgcatcgtcg ggcaccggca
    70441 ggcccagatc ggtgctggcc gactggatgg cgcggacgat ggcggccgcc gaatccatca
    70501 gcgtcccgtc ccagtcaaag acgatcagat cgaatcgctt aaccatagtc ccgctcctca
    70561 gcctgttcca cggccacaat gaaggccgca agttcgtccg gcagcggcgc cgaaagttcg
    70621 atcggcgcat cggtcagcgg atggcggaag gccagatgcg ccgcgtgcag gaacatgcgc
    70681 ttcagtccgg ccttctcgag ctgcttgttg agagcgaagt caccgtactt gtcgtccccg
    70741 tacaaaggaa agccctggtg cgtcaggtgc acgcggatct ggtgggtacg cccggtcttc
    70801 agctccacct ccaccaggct gcagctctgc cagcgcctgt gcagacgcac gatgctgtgg
    70861 gccggcttcc cgtccgggtg cacactgacg cgccgctcgc cgccttcggt cagatatttg
    70921 agcagcggcg ccttcacatg ttgcagcgga ttcatccagc gccccgcaac gagggtcagg
    70981 tagcgcttct gcacccgccc gtcgcgcatc atgtcgtgga gcaccgtgag cgcgctgcgc
    71041 ttcttggcca cgatgagcag tccggacgtt tcccggtcga gacgatggac cagctccaga
    71101 aagcgcgcct ccggccgctg ccggcgcagc tgttcgatga caccgaagct cacgccgctg
    71161 cccccatgca cggccaggcc ggccggcttg tccagcacca gcagcgcctc gtcctcatag
    71221 gcgatcgcga aagccttgcc caccggcacc ggcgagctgt cctcccgctc ggccagacgc
    71281 aggggcggga tgcgcacctc gtcaccggac tgcaggcgat aggtctgtgc cacccggcgg
    71341 ctattcacgc gcacctcgcc actgcgcagg atgcgataga cgtgactctt cggcacaccc
    71401 ttgcaatggc ggatcagaaa attgtcgata cgctggccgg cggcggactc atcgaccatt
    71461 acgcgactca ctgcgggggc tttactcaag gccatcgttt tgaaatatac tgtctagcgg
    71521 ctttttcggt ccggaaccgt cgcgcctgcc cacgacattc gttgatgcgc gcactccgtc
    71581 tggcacggag tatcgaaatt caacgccggc ccgcgcccct ttccagagcg atgtgcggaa
    71641 gcgcccactg cgccttccct gctcccgaaa aagtcacagg aaggtccgtc gccagacaaa
    71701 agagggcgat ggtactgtat tcgacaataa acttgcacct attgcgcgca ggcgcacacg
    71761 cagattgacg accctgaagc cggcgcagaa cgcggttcag ggtgcgactc agacgaattc
    71821 cgccgccccc gttagtttga ttcccccctc aatgaaggat ggtcgcgatc acgccaaact
    71881 aagtcaccgc tcctaggcgg tggcgcggtt cgtcctgccc gtgtgtctcg cgggagaaaa
    71941 agacgaatga agcgcatgct cttcaatgca acgcaggcag aggaactccg cgttgccatt
    72001 gtcgatggtc agaaactcat tgaccttgac attgaatctg ccgccaagga acaacgtaaa
    72061 agcaacatct acaaggccgt catcacgcgc atcgagccca gcctcgaagc cgcgttcgtc
    72121 gactacggcg ccgagcgtca cggattcctg cccttcaagg aaatctcccg cgcctacttc
    72181 cagccgggtg tcgaagccag ccgcgcctcc atcaaggaag ccctcaagga aggccaggag
    72241 ctgatcgtcc aggtcgagaa ggacgagcgt ggcaacaagg gcgctgccct caccaccttc
    72301 gtctccctgg ccggccgcta cctcgtgctg atgcccaaca atccccgcgg cggcggtgtg
    72361 tcgcggcgcg tggaagggga cgagcgcgcc gagctgcgcg acgtcatgga ccagctcgaa
    72421 gtgcccggcg gcatgagcct catcgcccgc accgcggcca tcggccgcag cgccgaagag
    72481 ctccagtggg acctcaacta cctgctgcag ctgtggcgcg ccatcgacgg cgccgcccag
    72541 gcccaggccg gcgccttcct catctatcag gaaggcagcc tcgtcatccg cgccatccgg
    72601 gactacttcc agccggacat cggcgagatc ctcatcgaca ccgacgacgt gtacgagcag
    72661 gcccgccagt tcatggccca cgtgatgccc accaacgtga accgggtcaa gcgctaccac
    72721 gacgacgtgc ccctgttctc ccgcttccag atcgagcacc agatcgagtc ggcctactcg
    72781 cgcacggtca acctgccctc cggcggcgcc atcgtcatcg accacaccga agccctggtg
    72841 tccgtggacg tgaactccgg ccgcgccacc cgcggcgccg acatcgagga aaccgccttc
    72901 aagaccaact gcgaagcggc cgacgaagtc gcccgccagc tgcgtctgcg cgacctgggc
    72961 ggcctcatcg tcatcgactt catcgacatg gagtcccaga agaaccagcg cgaggtcgag
    73021 aaccgcctgc gcgacgccct gcgctacgac cgcgcccgcg tccagaccgg caagatcagc
    73081 cgcttcggcc tgctcgaact gtcccgccag cgcctgc
//
LOCUS       DAOQPA010000005        71433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_122_length_71433_cov_19.088893, whole genome shotgun sequence.
ACCESSION   DAOQPA010000005 DAOQPA010000000
VERSION     DAOQPA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 71433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71433
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_71433_cov_19.088893"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKX01_01390"
     CDS             complement(<1..711)
                     /locus_tag="PKX01_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ74799.1"
                     /translation="MRIRPLSIALSVLLLSAAPTGWAAETLADAWAQALSVDARLRAA
                     DAEGEGARATLEAAKGLALPKATASSRYTVLSKEPAALIGLPTLPGISLPDRMPLSER
                     TYATHALQASLPIYTGGRIPAAVAAADIGVQASAQERLRTEQTVKLDVAEAWLNVLRS
                     RRALAAAERHVAALDAHAADVNALHRQGVVPRNDVLSVAVALADARQQQTRAWHGVDL
                     AAAAYNRLLGRPLETAVDV"
     gene            1064..1552
                     /locus_tag="PKX01_01395"
     CDS             1064..1552
                     /locus_tag="PKX01_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74800.1"
                     /translation="MPLPALDDDLVDFGDIPAPINALLQEGVYWHPADPARARACFRS
                     AIDLAPDVLPSYRCLFKAHNKQRDFAAAYAVALEGLGRAAMQAGMSPDWSTWTRPALA
                     AAPTVPARFLLHSLKAVAFIELRRGHESASRAALAHLQALDPEDGVGASVIAALLDRE
                     DA"
     gene            1552..1884
                     /locus_tag="PKX01_01400"
     CDS             1552..1884
                     /locus_tag="PKX01_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74801.1"
                     /translation="MRRESDTPPFAVTQTVHRALGPLVVLEVGALKVRLTPREAHTLA
                     LALFAVRDGRSAETELFMSPIASDGIFVAPVGSDGIAVETSAGPVALDWQAVGELGAQ
                     LSPAAVPG"
     gene            2081..4447
                     /gene="parC"
                     /locus_tag="PKX01_01405"
     CDS             2081..4447
                     /gene="parC"
                     /locus_tag="PKX01_01405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HMZ74802.1"
                     /translation="MNDTPDLFDTLPPDTPEPPPPPVATPLAGSDDALPLDLYAERAY
                     LSYAMSVVKSRALPQVEDGLKPVQRRILFAMNEMRLSPASKHVKSARVVGDVIGKYHP
                     HGDSSVYDAMVRVAQDFSLRYPLVDGQGNFGSRDGDSAAAMRYTECRLTPIAELLLSE
                     IDRGTVDFRPNYDGAFQEPQLLPARLPFVLLNGASGIAVGMATEIPSHNLREVARAAI
                     HAIEHPEATVADLLGHIAGPDFPGGGQLISPAADIRQAYETGRGSLKLRARWIVEDLA
                     RGQWQLVITELPHGVSAQKILFEIETLTNPQPKTGKKSIDPDQAQLKALILSALDRVR
                     DESGKDAPVRLVFEPKSRNQDVAEFANLLLAHTSLETSVALNLVMIGLDGRPAQKNLA
                     DILHEWGRFRTVTVRRRSVHRLGQVDDRIHILEGRHIVFLNIDEVIRLIRESDDPKAA
                     LIARFDLSDRQAEDILEIRLRQLAKLEGIKIERELAELRGEKTELEALLADEGKLRRL
                     VIKEIRADAEKYGDARRTLVEEAARAGVTQTVVDEPVTVIVSEKGWVRARSGHGLDLA
                     NVAFKDGDRLATALECRTVDALIALTDGGRALTVAVSTLPDGRGMGAPLASFVELPAG
                     AKIVHVLSGDPASRVLMATRDGYGFLCRLGDMLATKKAGKQFVTLDEGVGLLPPLIYA
                     DAPDLHVATLSEKGRLLVFTLDQVKELSGGGRGVILQALEDGDTLRAVCVAGSDGIVL
                     SSASSAGRAYEVPIPARDWTPWLNKRARKGKPAPGRFTPTAVTPLKRD"
     gene            complement(4434..4979)
                     /locus_tag="PKX01_01410"
     CDS             complement(4434..4979)
                     /locus_tag="PKX01_01410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ74803.1"
                     /translation="MAAELTEICVRSEQVFDGVLLNVHRDTVRLPDGKQGVREYIRHP
                     GAVVVIAQLPDGHLIFERQFRYPCGRVFIELPAGKIDAGEDIERCARRELLEETGYVA
                     DSWRHLGVMHPCIGYSDERIEIFLASGLRHEGASLDDGEYLEIFPMSLDEAEAAVLDG
                     RITDAKTITALFWAAKVLSPA"
     gene            complement(5087..6271)
                     /locus_tag="PKX01_01415"
     CDS             complement(5087..6271)
                     /locus_tag="PKX01_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HMZ74804.1"
                     /translation="MASRDVVVLSAVRSAIGAFGGSLADFDASELAGIVMKESVVRSG
                     VDPQLINYVTVGNCIPTDARYAYVSRVASIQAGLPMESVAMQVSRLCSSGLQGIVTTA
                     QNILLGDADYGIGGGVEVMSKATYMLPALRSGARMGDTKAIDSMVAVLTDPFGVGHMG
                     ITAENLAVKHGISREEQDAFAVESQRRAAVAIDEGRFKSQIVPIVKQTRKGDVVFDTD
                     EHVKRGTTLESLAKMKPAFKKDGTVTAGNASGINDGAAFFVLADGATAVNAGYKPMAR
                     LVSYAIAGVPNDVMGEGPIPASKLALKKAGLTLDQMDVIESNEAFAAQALTVMKVLGL
                     DPVKTNPNGGAIALGHPVGCSGAFIATKALYELQRTGGRYALVTMCIGGGQGIATVFE
                     RI"
     gene            complement(6298..7227)
                     /locus_tag="PKX01_01420"
     CDS             complement(6298..7227)
                     /locus_tag="PKX01_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HMZ74805.1"
                     /translation="MTLFSLIVALLIEHLRPISTERFVEAPLKAFAHFLEDRFNDGEA
                     RHGAVAWWVAMLSVGFVSALAYFVLWHVHPLLAMVFNVVILYFTMAFRNLSRFFSDLQ
                     LALRMGELARARQLIGVWRKRSHDASSSTEVARLAIEEALIAAHRNVFAVIFWFILLP
                     GPSGALLYRLARFFDEEWSLRKGEPEFGDFGEFAHRAFAALDWLPVRLSAAAFSIVGD
                     FEDAVYCWRAQAAQWADKASGILLASGGGALGVRLGMPVHESGEIVERPEMGIGDEAD
                     ADFMQSTIGLIWRTLVLVFLVLALLGVAGWVGH"
     gene            complement(7320..7904)
                     /locus_tag="PKX01_01425"
     CDS             complement(7320..7904)
                     /locus_tag="PKX01_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMZ74806.1"
                     /translation="MSLSCPTADQLRRRFAESPAIETPVEEGVPEELRPAAVLVPLVM
                     RDTPTVLLTRRTDHLHHHPGQVSFPGGRMEEHDASPIETALRETEEEIGLQRQHVELL
                     GHLPVYRTGTGFEVTPVVGLVAPPFDLKVDDFEVAEVFEVPLAFLLDSANHQRHRVVV
                     RGALREYYAMPYGNYYIWGATAGMLVSLQRFLSA"
     gene            complement(7922..8530)
                     /locus_tag="PKX01_01430"
     CDS             complement(7922..8530)
                     /locus_tag="PKX01_01430"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HMZ74807.1"
                     /translation="MAAMDLAQATDVVLAGVFPGKALLVIDGRAPRAVASGAMTPEGV
                     RVITVEGDTATVEVDGRRQRLVVGQSAVRVGGETSGGGTVELQADRAGHFLTYGTVNG
                     AQVNFLVDTGASLIVLGRSDAIRAGIDYKKGEPAQSITANGVARTWIVMLDSVRVGNV
                     TQRNVRASIHGEDLPVALLGMSFLNRMEMRRDNGVMTLKQRY"
     gene            complement(8670..9434)
                     /locus_tag="PKX01_01435"
     CDS             complement(8670..9434)
                     /locus_tag="PKX01_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HMZ74808.1"
                     /translation="MAGLAQRRRVLAGLGALATTWAMPTWADFPDVVAQIKNGVVAVG
                     TFLPSRNPQFKFRGTGFVVGNGNLVATNIHVVSDALEENETLTVALPGTGRVIGRAVV
                     RQASDADHDLVLLRFDGPPLPALRLGDSGAVRDGQEVAITGFPLGASLGLVPVTHRGI
                     VSAVTPVGMPAPSASQLDARTVRRLAMGAYNVFQLDLVSYPGNSGSPLFDTHSGEVVG
                     ILNMVFVKGTKESAVAMPTGISYAIPTHYLKALLVP"
     gene            complement(9437..10249)
                     /locus_tag="PKX01_01440"
     CDS             complement(9437..10249)
                     /locus_tag="PKX01_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ74809.1"
                     /translation="MLVEQAVAFEHGEGVPRDLERAAVLYCEAARAGDPEAMYSLGWM
                     YLNGRGVLRDDAVATTLFDMAIVKGHDGALRARRFASDYTGAVPDCLKDRPADHGFAA
                     RDSWDTQKYLASLPDQKKKVVEILTTLAPRFSIEPRLALAIASTESNFDTGARSPKNA
                     MGVMQLIPETASRFNVRDSFDPVQNIKGGLAYLRWLLAYYRGDVALAVAGYNAGEGAV
                     DRYRGVPPFKETQNYVRRILAFFPRTDHPFDARLVDASPIVQTFRAVTRFKD"
     gene            complement(10369..11649)
                     /locus_tag="PKX01_01445"
     CDS             complement(10369..11649)
                     /locus_tag="PKX01_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ74810.1"
                     /translation="MTTIAVIGLGYVGLPLAVEFGKKMKTVGFDLSAEKVAAYGRFVD
                     PTGEVSTDDLQAAVHLTCSTDPKIIGEADFIIVAVPTPVDDAHQPDFTPLVKSSESVG
                     KHMKKGAIVVYESTVYPGATEEVCIPILEKFSGLKWKQDFNVGYSPERINPGDKERTV
                     TKIMKVVSGDTPETLKIVADTYAAVIVAGVYPASSIKVAEAAKVIENTQRDLNIALMN
                     ELSVIFHKIGIDTLEVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKADMLGYHPQ
                     VILAGRRINDSMGKYVAEETVKQMIRSGQNIKGADVIVLGLTFKENCPDLRNSKVIDV
                     IRELQSFGCNVHVHDPVAESAEAEHEYGVSLTSWDKLPKAGAMVAAVAHKEYAEMGVG
                     ALAEKIVSGGVFTDVKSFYDPAQLQAAGLSVWRL"
     gene            complement(11690..14470)
                     /gene="prsT"
                     /locus_tag="PKX01_01450"
     CDS             complement(11690..14470)
                     /gene="prsT"
                     /locus_tag="PKX01_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HMZ74811.1"
                     /translation="MQKGSGRNVVRSATVSVLCALLLAACGGSPESMVASAKEYLAKN
                     DRNAASIQLKNALQKNPGFGEARFLLGKINFDSGDYAGAEKELRRAMDAGFSLDETVP
                     LLAKALVLSGQGDKVAELANQSLSDPQAKAALMVAQGEALITKNKRDDAIQLYDSALR
                     LVPGFPTARIAKARIGAFDHRYDEALAEVDDVLKQSPNLPEALSLRGDLMLALNRVDD
                     ALKAFEALVAVRPTDVIAHQNVVSLLLRQNKLDDAGAKIAAMRKALGNHPMAVYLQAF
                     LDTRQGKMKEAYDELQQVLRVAPDYVPALMLMATLQLQRSEFVEAQANLAKIVDRFPG
                     ATLARRLLVSAYVGLREPSKALETLQPLIKDRTEDVTILNLAGQVYAMAGDFARSEEY
                     FGRAAASDPKNAQLRTRLGASRLAAGETDRAFQDLEAASALDPDNIQADVTLIMAHLR
                     RNEVAKALAAVAVLEKKKPNDPVTFNMKGGVLLANKDIPGAREAFEKALSIKPDFLAA
                     VTNLARLDVADKQLERARKRYEEFLNRAPKDPNGYLQFAEFTAQTGSQPKDVQAILER
                     GVTAVPTALPLRLALVRMLVQGGDTKRALSLAQEAAAAAADDAAVLELLGRAQVAAGE
                     LQQALTTFGKLAAKLPTSPGPLIAMANVQIASKDLSGAEQSLRKALAVKPDSVDAQQR
                     LFAVLMTGKRFDDARRVARDIQKANGKAAVGYVLEGEAYTAAGKPTEAVTAYEEAFRL
                     DHSAQSCIWLHVARLAAGQSAEAAKLATDWLKGNPKDLIFRAYLAERSLKEQKFDQAA
                     QQYRQMVDIAPKNPLLLNNLAWAMGKQGDKGALQVIDKALAMTPNSPVLIDTLGTLQI
                     QFGDAEKGIQNLRKAVSLGPKLPQLQVSLVSGLIKAGKKDEARTEIEAALKTAPENSP
                     QKAELERLRKTL"
     gene            complement(14488..15861)
                     /gene="prsR"
                     /locus_tag="PKX01_01455"
     CDS             complement(14488..15861)
                     /gene="prsR"
                     /locus_tag="PKX01_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HMZ74812.1"
                     /translation="MSDKRRTLLIVEDDPALQKQMRWAFDQYDTVVASNRDEAIAQIR
                     RYEPAVVTMDLGLPPAPDDVTEGFRLLGEILALMPDTKVIVLTGQHDRENAVRAVGMG
                     AYDFFAKPFEPEMLVLTLERAFRLYDLQQDNRRLKMQQGSALSGVITRDPGMLRVCRL
                     VEKVASANVTVMLLGESGTGKEVLARGLHELSPRRNERFVAINCAAIPENLLESELFG
                     YEKGAFTGAVKQTLGKIEVANKGTLFLDEIGDLPMPLQAKLLRFLQERVIERIGGREE
                     IPVDVRIICATHQDVKGLIQTGRFREDLYYRLAEIVVDIPALRDRDGDAVLLAHAFVQ
                     RFAASNGRGTMTLTDDAVQAIESHRWPGNIRELENAIKRSVIMADGNCISADDIGVGD
                     GTVEEGELEALNLRVVRDEAERRAVIRVMARVNGNVARAAEVLGISRPTLYDLMNRFG
                     LKKETST"
     gene            complement(15858..17963)
                     /gene="prsK"
                     /locus_tag="PKX01_01460"
     CDS             complement(15858..17963)
                     /gene="prsK"
                     /locus_tag="PKX01_01460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HMZ74813.1"
                     /translation="MELFLSQVSVWSYAFATVLHGLFALYLGIAWRRGRRGWLLVAAV
                     VLSGGWAAASWGFALTGWIWLFQLGNLFDVLRQAAWFGFLVAMLLQNASEADGNVAAL
                     AWPRPAMLAIVALAAGGVACFTLGIPLYGTPMRTWAATSLASSVFGLVLLEHFYRSVP
                     ERSHWGIKPLTLALGGLFAFDVYLFSDALLFTRLHPSVWSVRGFAHTLAIPLIAVSAS
                     RNPEWTFRISVSRHVVLHSTAMAGTGLYLTLIAGAGYYMRYFGGEWGQALQVALLFGG
                     ALFLGVVAFSGAVRSRFKVLIAKHFFAYRYDYRTEWLGVTQALSAAGDGLTLGESVVN
                     ALGDLVESPGGGVWLKDAAGRYSQQARLNMPHCGMQVGDDDAFIRFLLEKEWVVNLEE
                     ARAGVNFGESQPVPAWIGEVDDAWLIVPLISAGSLIGFVILLTPRTPVDVNWEVLDLL
                     KTAGRQAAGYFARMQAAEALLEARKFDAFNRMSAFVVHDLKNLVAQLSLMLRNAERHK
                     DNPEFQADMLDTVRHVEERMRGLMAQLMEKTPINQRKPVPLTPLLEHIVQSKRLFRPA
                     PVLVSGGPDLAVLAHSERLERIVGHIVQNALDATSEHGSVSVEVSRSGNFASIRVADT
                     GSGMSAAFIRDQLFKPFQTTKASGMGIGAYEARQYIHELGGSLSVESEVGAGSCFEIR
                     LPLAHLPGGVDGEPAAKQT"
     gene            complement(18041..19429)
                     /locus_tag="PKX01_01465"
     CDS             complement(18041..19429)
                     /locus_tag="PKX01_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HMZ74814.1"
                     /translation="MLKLFNHYVPSNTILQLMFDVTLLFVSVVVVFLLQTQLDESLVR
                     VVVPSALVFAITMMVLNGALGLYRAGSGADLRETTVRVLISIVLSVPVAYGIFRVLPW
                     GEFAHEAMELDVIALLGFVVAVRGFTMRKMGVGVLAKRVMVIGTGADAVAVEHALTHP
                     ANPSLQVVGFFPAGSGGDEVRVDPRRIVHYDGRLLAAANDLRVNEIIVAVKERRGGVL
                     PLRELLECKMAGIKVLDLSSFFERVRGQVRIDSLRASWLIYGEGFRQGLGRTLVKRVF
                     DIVASLILLALATPVMLLAMLLIVMEDGAPVFYRQERVGLNGRLFKVTKFRSMRRDAE
                     RDGKPRWASANDDRVTRFGRIMRKTRIDELPQLFNVLKGDMSLVGPRPERPFFVDQLT
                     KDIPFYAVRHCVKPGVTGWAQVRYQYGSSVDDAINKLQYDLYYVKNHTLFLDIVVLFE
                     TVRVVLTGDGAH"
     gene            complement(19849..21744)
                     /locus_tag="PKX01_01470"
     CDS             complement(19849..21744)
                     /locus_tag="PKX01_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HMZ74815.1"
                     /translation="MSAVVAEDQHGHSGTKVLPVALAAMGVVFGDIGTSPLYTMKEVF
                     NGPHAVPATPENVLGILSLVFWALTFVVSLKYVVYIMRADNRGEGGIMALTSLALRST
                     DSPRAASLMAALGLFGAALFYGDGVITPAISVLSAVEGLEVATPAFRPFVIPISLVVL
                     IGLFVIQRKGTAQVGAVFGPVMLFWFATLGVLGAFNVVRHPDVLGALNPLHAVRFFVE
                     NRVHGFLALGAVVLAITGGEALYADMGHFGRRPIKLAWFGFVFPCLYLNYLGQGALII
                     DDPSAVANPFYLLVPDPLLYPMVGLSTVATIIASQAVISGAFSLTKQAVQLGYSPRIH
                     IVHTSDREAGQIYVPNVNWMLLGAVIALVIGFKSSSALASAYGIAVTLTMMIDTVLAF
                     VVVRALWHWRWSAAVAFVALFFSVDFSFFAANSVKILDGGWFPLALGLGVHTLLMTWK
                     TGRALLTERMRVDTMPLDLFIQSMFHDPPHRVPGLGIFMTTNPDGVPRALLHNMLHNK
                     VLHASVVLVHVVTADVPYVPLEERVRLEELDFGFFRVFVRYGFKDDPDIPAALALCAE
                     KGLPYELMQSSFFLGRETLIPRKGSAMPLWREHLFNIMFRNADSAADFFRIPVNRVVE
                     FGTQIEL"
     gene            complement(21860..23239)
                     /locus_tag="PKX01_01475"
     CDS             complement(21860..23239)
                     /locus_tag="PKX01_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ74816.1"
                     /translation="MNSIDRRARASGPDVLIVDDEDDIRELLELTLMRMGLACDGAGT
                     VADAQRLLTERRYRLCLTDMRLPDGDGLDLVRFIADKVGDLPVAVITAFGSMDNAVTA
                     LKAGAFDYLSKPVSVDQLRALVKSVFSVSPADGVEGTRDTSSLGGHSAAMVQVRALIE
                     KLARSQAPVLISGESGSGKERAARMIHELGPRSGAPFVAVNCGAIPESLMESEFFGVK
                     RGAFTGADADREGFFQAAHGGTLFLDEVGDLPLPMQVKLLRAIQEKRFRRLGDTTEVG
                     VDVRLISATHRNLKSMVEASSFRQDLYYRLNVIELRMPALRERSEDIPELATTLLGRL
                     AENSGMDVPRLGPDALQALVGYAFPGNVRELENILERALALSNGNVITAADLLLEPAE
                     ELAGESPSIVVQDGGAPLQDYLDHVERQAIVEALERAGGNRTAAARALGVTFRSLRYR
                     LERLGMKDG"
     gene            complement(23236..24816)
                     /locus_tag="PKX01_01480"
     CDS             complement(23236..24816)
                     /locus_tag="PKX01_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ74817.1"
                     /translation="MAYPGLESGATEFRGRWEPLRYLNLFRLVLAGVFLVAGPGLSLG
                     GDSATLFFGAAFAYLTAVLILGFPDAARHLGMERLVTVQVLVDVTLLTVIMWASGGYR
                     SGMPILIMIVLAGSGLVGQGRMVLFYAAYATVAVLFENLLRFLSGRDVVDFFTVGVTC
                     IGFFTIAQVARLLALRAIANETLARQRGADLAAQLRLNQQIIQDMQDGVLVVEPGGVV
                     RHFNPQANALLGGTLREGGALASCAPALAGPLGAGEDGEPGAHSALRFRAEGSGKLLL
                     ARMLRAGDSDATLVYLEDFDLIHDQIQQVKLAALGRLTASIAHEIRNPLAAVSHAAEL
                     LSDEKRGDMQARLIRIIGTNAQRIDRLVGDVLALGRRDEALPEVLPLKQFVDEFLDEF
                     ALTDPVRRALVDVAIASDLTLLMDRTHLRQILWNLLANAQRHGSGAPGSIRIAAGLRD
                     DGRIAVDVTDDGPGIAEALRNQVFEPFFTTHSKGTGLGLYIARELADANGAVLELGQA
                     EAGGLPGAHFRLTARSRS"
     gene            complement(24822..25076)
                     /locus_tag="PKX01_01485"
     CDS             complement(24822..25076)
                     /locus_tag="PKX01_01485"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HMZ74818.1"
                     /translation="MRNLLVFVLFLIGLYYVRRALQGKSGDTPGAGREARSEAPRQPT
                     EAEAMVACAHCGVHVPESEGLHVGGYFFCSDAHRRLGPRV"
     gene            complement(25105..26265)
                     /locus_tag="PKX01_01490"
     CDS             complement(25105..26265)
                     /locus_tag="PKX01_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ74819.1"
                     /translation="MPVFSAPLVSRLPTVGTTIFTRMSQLAQTCGAINLSQGFPDFSA
                     EDVLFERVAHWMRAGFNQYAPMAGMPALRDAIATKVHALYGAHYDPGAEITVTAGATQ
                     ALFTAVAALVHPGDEVIVFEPVYDSYVPAIELQGGVVRRVRLGAPEYRPDWAALRALI
                     TPRTRMIMINTPHNPTGAVWSAADMIELAAVTRGTGIVVVADEVYEHIVFDGARHESA
                     ARDPELAARSVIVSSFGKTYHVTGWKVGYVLAPQALMDEFRKVHQFNVFTVNAPVQLA
                     LADYMAEPSRHLALAAFYQAKRDAFRAGLAATHFELLPCAGTYFQTVRYAAVSDLPDL
                     AFVEWLTREVGVAAIPLSAFFGDGRDEGVIRFCFAKQEATLAEACERLARFC"
     gene            26351..27448
                     /gene="mtnA"
                     /locus_tag="PKX01_01495"
     CDS             26351..27448
                     /gene="mtnA"
                     /locus_tag="PKX01_01495"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMZ74820.1"
                     /translation="MQTPSNAPVTDTCPTLRREGDSVLILDQTRLPHEEAFVRLATLA
                     DAALAIRSMQVRGAPLIGATAAYGVALALASDASDAALADADACLRTTRPTAVNLHWA
                     LDRMGRKLASVAPAGRREAAWGEADAIRREDADANAAIGQHGLALLNALAAEPGRPVH
                     VLTHCNAGWLATCGHGTALAPVYAARAAGLPVHVWVSETRPRNQGLLTAWELRRDGIP
                     HTLIADNAAAWLMAEGRVDCVIVGADRIAANGDTANKVGTCLKALAARAHALPFYVAA
                     PLSTVDRSCPNGRAIPIEARDGDEIRRIGGLDDNGRPAFVHIAPPDSPVCNPAFDVTP
                     AALIDGIVTPVGVCAAHDIGRLAGSLSGDSP"
     gene            27445..28149
                     /locus_tag="PKX01_01500"
     CDS             27445..28149
                     /locus_tag="PKX01_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HMZ74821.1"
                     /translation="MIENVLREALLATAQSMGSLGLNTGTAGNASVRCHDNLLITPTG
                     CDYASCTPSDMVLMAMDGTWQGHCAPSSEWRIHRDILAARPEAGAVLHAHSPFATALA
                     CHRRGIPAFHYMIARFGGTDIRCAPYATFGTQALSDGALAALEGRSACLLANHGMLVI
                     GSDLRRALALAVEFESLCAQFWRACQIGTPTLLSDAEMADVLAKFRHYGQPAQAALLA
                     DALPAAGPAASGLSDG"
     gene            28162..31254
                     /locus_tag="PKX01_01505"
     CDS             28162..31254
                     /locus_tag="PKX01_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ74822.1"
                     /translation="MHPHSRSVLLGIAGASFIAAVLAWHLLGDHPAFFLPVLAVLMAA
                     PVLACLVFLGRPADTARQAWRLLESSPIAVVINRPDGTPLFANTRAHDLAGTDAAGFM
                     ARPVMSWFGDPDFPQAALERLRAGERLHDMEVEFQTVRGDSFWTLTSMESLDLDGRPA
                     IISWSYDITARKTAEQELRKLSLAVEQSPSMLLVTDPDGRIQYANPCYCRTAGLTAAE
                     LVGQPPDLVDLTDAPLRFSAPRHPELKDGGVWLQECRLRTAHPRTQWVRVMVSPLIED
                     ATIRHCIWVLEDLSLHRDAMEALDQAKRLAEEAAASRARFLADMSHEIRTPANAIVGL
                     THLALDSGLPPAQLDHLTRIQSAADTLLAVINDVLDLSKIDAGRLSLEQAPFSLDEMI
                     DRVITLVAPAAHARHLALLLAIAPDVPLRPVGDALRLGQVLTNLLANAVKFTERGFVR
                     LNVVVAARDETSASLHFEVEDTGIGLPPGEVEHLFEAFSQADDSVTRRFGGTGLGLAI
                     SNHLVTLMGGRIEVRSAPGDGSRFGFTVRLGVAPAPAAVELPSALRGAHTLVIDPHTA
                     ARDLLVAQLNALGVPTEGCDTPDAALAWAHDPDRSIRLVLAAAPRGESGPSLTAALHT
                     LAGHGPLPALVIMGPFPAHRDHAGLPAGHLRIPATRSRLRDAVVEALAPTRPEPHELP
                     APPPRALAGRSILVAEDVEINRTITQNFLENAGARVTLANDGDEALRLLQTMGAQAFD
                     AVLMDIQMPVLDGLSATRQLRADSRFAHLPVIAMTAHAMADDRDRCLQAGMTDHVAKP
                     IAPERLIDVLIRNTMRAPQPAPAAGPQPAAVSHPSDLPHIAGIDVPGALPRFNHNVDM
                     YLRILRRFAPHHGDAPARIEAALAAGQRDDAERIAHTLRSSAAGLGADGIADLAAQIE
                     LGLRKGAGIDTPLHGLHEEMTRLLAALDRALPPEEAPRPGAPELDAESLDAACRELDA
                     LLRHADGRAPALFLRIRNDLSRRHAPAAVETLADAIARYDYDKALIHLANVMNRAPAP
                     "
     gene            31287..32456
                     /locus_tag="PKX01_01510"
     CDS             31287..32456
                     /locus_tag="PKX01_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HMZ74823.1"
                     /translation="MEQTDPTSDQDDHRAIILVVDDAPAAIEPIVDCLHRADFRTRIA
                     TTGERGLALARVEPLPALILLDVIMPGLDGYEVCRRLMADPVTANIPVIFLTARNDVV
                     DEQAGFDAGAVDYITKPISPPTVLSRVRNHLVLKSASDFLRDRNTYLEQEVLRRTREL
                     SLIQDATIVAMASLAETRDNETGNHIRRTQMYLRALARHLQHHPRFAAELSDENIDLM
                     YKSAPLHDIGKVGIPDRILLKPGRLTADEFEIMKTHTTLGRDAISAAESLFGVSARFL
                     RYAKEIAYSHQEKWDGSGYPQGLSGDRIPLSARLMAVADVYDALICRRVYKPPMSHAD
                     AADIMRAGRGTHFDPDILDAFLALLDDFHAIALRFADLEQDHAREIERLRQVFGD"
     gene            32564..35395
                     /locus_tag="PKX01_01515"
     CDS             32564..35395
                     /locus_tag="PKX01_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ74824.1"
                     /translation="MISSTTSRQVVDTIRRYGLALLAFALVLLVSFERYHSTVQQEQA
                     TTHTRFQLMAQEQIARLRERTEELFSHADVLQHYAGQGGSLDPQRFARLLMPMVAHNP
                     EIASAGILRRHADGAPVEVLQVTGATRLTRGADFPNSEAYPDADDRRILLEALRNAPH
                     TATGTATLPVATRDIGGTPADTFLIFAPVREDPDAPPGNQQDAAFLEIRLSRLIDAAF
                     QPAINRDVNQSIGVEVLDATDEARTLLSSPGFRPAELTYADAVPMGNRRWLVIIVPDG
                     PHYSLSPSADAGGQLFAGFSLAALIAFMVYVLQSRTEAIRHRVAEQTVALAEANDALG
                     QYVNALGETNRLLENEVDKHARAEALLRETTSLQRAILDCAEYAIIYTDTAGIIRVLN
                     PAAERLFGYRAEDVIGRDTPLIFYTAEDIAGLTLAGKPSGFAALAVEPLNGAADTPTG
                     AADPREVTLRRRDGETFPANIVVSTVRDASRNVRGYLGIVADITQRRAAESRIRHLAH
                     YDNLTSLPNRTLLNQHLTEVIEQAAVDQRRVAVLFTDLDRFKYVNDTLGHQAGDQLLL
                     SVAQRLRGCVRDGDLVARTGGDEFVVVLSRLEHAGGAEEAAGRILTALARPFELCGQQ
                     FTVTPSIGIALYPNDGLDGEALIKNADAAMYLAKERGRNNFQFFDHGLSERYSERLEL
                     EARLRQALQAREFELYYQPQVDTLSGEIIGMEALIRWPQPDGSLIPPDKFIPIAEECG
                     LIVPIGAWVLEEACRQNQAWIREGIARVPVGVNLSARQFDDKDLLDTIKSALDTAGLA
                     PAYLDLELTESLVMRNPEHTRNLLVECKRLGLQLSVDDFGTGYSSLANLKRFPIDRLK
                     IDRSFIKDIASEPDDATIAQTIVAMAHTLRLEVIAEGVETEAQLALLRRWRCDAYQGY
                     LCSRPINAAAMTQMLRNLRASRLVSLTAL"
     gene            complement(35408..35881)
                     /locus_tag="PKX01_01520"
     CDS             complement(35408..35881)
                     /locus_tag="PKX01_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HMZ74825.1"
                     /translation="MERGRRRFLTVAAAALTALGGCAVMRLGLDAPEVRVAGIQPVDA
                     TLLEQRFLITLRVLNPNNVEIDVEGLTFAVELNGHAFARGVSNQATVIPRLGEATLEV
                     TATTGLMDVLNQLRQFQKAPEAVPYRLKGRLVTGRFGSIDFDRSGEVSLTRLQPR"
     gene            complement(35884..36762)
                     /locus_tag="PKX01_01525"
     CDS             complement(35884..36762)
                     /locus_tag="PKX01_01525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ74826.1"
                     /translation="MRVGFVGLGVMGRPMAQHLLAAGHDLAVWARRPASAEALRAAGA
                     AWCESPAALAARSEVVISIVTACADVEALALGADGLLAGFAPGSVHVDMSTIAPATAR
                     QLAGCYAERGVGWLDAPVSGGEVGARDATLAIMAGGEAAVLAQVRPLLDCLGRTVVHI
                     GPAGAGQVAKACNQMVMVNAIQACAEAMCLANAHGVDPERLWTALMGGSAASRVLEVM
                     GGRMARRDFAAGIQSRLHHKDFAILMAAARQGGVPLPVAAQTWQQLNALMAAGGGHDD
                     TASLLKMLEPAGRHGA"
     gene            complement(36765..37637)
                     /locus_tag="PKX01_01530"
     CDS             complement(36765..37637)
                     /locus_tag="PKX01_01530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ74827.1"
                     /translation="MKVGFIGLGIMGRPMALNLLKGGHAVTVWARRADSMAPLLDAGA
                     TGAADAAAVAGDVDVVFSMVADAADVEQVALGPRGVADGARPGLIYVDMSTIAPAAAQ
                     SIAARLAERGVSMLDAPVSGGEVGAINGALTIMVGGDEAAFGTVAPLFVCMGKAATLI
                     GASGAGQVAKACNQILTGLGVMAVAEAFNFARKSGVDAARVREALLGGFAYSRILENH
                     GQRMLDRNFKPGFKAWMHQKDMNIVMQEAHRLGLMLPSSAATAQLFNAMVGSGLGEED
                     SIAALKLLEQLSGA"
     gene            complement(37676..38584)
                     /locus_tag="PKX01_01535"
     CDS             complement(37676..38584)
                     /locus_tag="PKX01_01535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ74828.1"
                     /translation="MTQRIAAVVVTFNRKALLLECLAGLLAQSRPVDRIFLIDNASTD
                     GTREALEGAGFLSHPLIEYVPMATNTGGAGGFHHGLKVAFEAGYDWFWLMDDDVEPLP
                     DGLADLLSFSERSGCIHGRRREADGSPNVWGRTFYPNCVLSRSIRDPLFSQGQLFQTV
                     SVGCFEGMLISHAIVDAIGYPEPSFFIALDDTFYGYLASKITPVFYVNKFVLQRKRAA
                     EFTEPSLVGRHVVHGMLGLYYLQRNRFLIARKLSSNPLVFFIATIAYSSWGVFRELLL
                     SRSPARAATVLRGVRDGLRLYFGAAA"
     gene            complement(38622..39878)
                     /locus_tag="PKX01_01540"
     CDS             complement(38622..39878)
                     /locus_tag="PKX01_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HMZ74829.1"
                     /translation="MASLRRTLASLYVIQAANFAVPLLTQPWLTRALGVDGYGRLNFA
                     LAVVAYFVLLADYGFNLSATRQIAVQRDDKAARSRTFWATLGVKALLAFVGGLMLLVL
                     AALSSTLGEISGLLALGYLAVIGSVLTPTWYFQGVERADFAALATLLPKALAIPLIVA
                     GVRGPADLPAAMLLTAAPGLLSGLWCLAELARSRAVTMQRPALADLRAALADGWHVFV
                     STAAISLYTTSNTVLLGWLSGPAAVGHFAAADKLLKAAQAATAPVSQALFPRVSHLMH
                     HDRAAALCTLRRLLKWQGAFTFAVSVGLFVLAPVLVRLLYGEAFEAAVEVVRWMAPLP
                     CLIGLSNVFGIQTLLPLGREKLFSRILLVSGVVNLGLLALLAPVYGAPGAAIAVTITE
                     AGVTALMGWAVRAHFAHRAPVDAARP"
     gene            complement(39888..41057)
                     /gene="glf"
                     /locus_tag="PKX01_01545"
     CDS             complement(39888..41057)
                     /gene="glf"
                     /locus_tag="PKX01_01545"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HMZ74830.1"
                     /translation="MSVDAIIVGAGFSGAVLAERFASQLGWKVLVLEQRPHIGGNCYD
                     EPDAHGVLVHRYGPHLFHTDNPRVWDYLSAFTEWRPYEHKVLSSIDGQLVPIPFNLNS
                     IDRLFDADTATCMREALVSRFGLGARVPILRLKEEDEPLLRELADFVYRKAFLNYTTK
                     QWGVKPEEISPEVTGRVPVVVDRDDRYFTDPYQAVPAEGYTKLFEKLLAHPHITVRLD
                     TPFNAVGSFDPDTGAVQFEGAPFGGLLVFTGMVDELFGRDRGALPYRSIRFDFQHHAV
                     PVFQPATTVNYPNEHRYTRITEFKHLTGQQCAGTSIVYEYPQAYDPADPEANVPYYPI
                     FDERSKAAYTAYADRLGGHTRVVAVGRLAQYRYFDMDDAVANALQVFDRIAAAQG"
     gene            complement(41104..42558)
                     /gene="wbaP"
                     /locus_tag="PKX01_01550"
     CDS             complement(41104..42558)
                     /gene="wbaP"
                     /locus_tag="PKX01_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HMZ74831.1"
                     /translation="MRRPQRPAGEVRGWLVLADALALGVAFGLALLLLLWLRGHRIGP
                     SFAQWWAAEGVNQALAFLLLLVLALVTFAWRGHYHRRQPFWDELLEIIRSLLLAALLN
                     GMVVLLAKWPVSRFLWPVSWGLALVLLPLFRNGMKSALMRAGSWQLPTVIVGVGENAV
                     AAWQALRSEHLLGFEVHAFVAVGPEAPAELVVAPGVTVPVARPDKLLPWLADQGHPHV
                     TVAVSEPELQEIAGQVELLTLHYKDLHIIPPIAGLPLFGLSANHFFSHEVLMLRVRNN
                     LASRPLRWLKRMFDIAGSACGLALLSPLLAYIAVQVKRQGGPGSVFFGHERIGLDGVP
                     FKCWKFRTMTHDSAEVLARLLASDPQAREEWNRDFKLKNDPRITPIGAFLRRSSLDEI
                     PQLWNVLKGEMSLVGPRPVVEAELERYGERVAFYLEARPGLTGLWQVSGRNETTYDER
                     VALDAWYVKNWNLWYDIAILCKTIRTVVSGHGAY"
     gene            complement(42560..43636)
                     /locus_tag="PKX01_01555"
     CDS             complement(42560..43636)
                     /locus_tag="PKX01_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ74832.1"
                     /translation="MAHILHVVESFGAGTLSMVSAIANRQAADGHRVSLVYSTREETP
                     ANWRALFDPRIATRHVPMTRAIRPATDWASAVALRRILRDWRPDVVHLHSSKAGALGR
                     LLSLVCPGPRWFFSPHGLSFLQKAAGRIKNAVFLTLEKLLARIPVTFIACSPSEAEQI
                     RTHLSRRVVVVPNAVDLAILPQARGGTGVVRIGTVGRVTTARHPELFAEVARRSGRDG
                     VEFVWIGGGEASGEAALRAAGVHITGWVDRARALEELGRLDIYLQTSRWEGLPVAVIE
                     AMGAGLPVIATRVVGNRDLVQDGDTGWLVPEDAAAFVARLGPLVNDPALRQATGARAQ
                     AFVRAHFAFDRMMEALYRAYGLQN"
     gene            complement(43644..43853)
                     /locus_tag="PKX01_01560"
     CDS             complement(43644..43853)
                     /locus_tag="PKX01_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74833.1"
                     /translation="MNFLVYLIPHYKIRLRLICSQAPIDGGEGCEVVVDGSVQVPIDE
                     CAAQRAFWDWVFALKSQLRRGARAG"
     gene            44245..45474
                     /locus_tag="PKX01_01565"
     CDS             44245..45474
                     /locus_tag="PKX01_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ74834.1"
                     /translation="MNALPSVSRPFSDRAETVALCLIVVCAFVVPLPTALISICTFLF
                     LLAWLAAGRLGERWATLHRHPQGPLCLALLTWMGVSIAWSPAPFHEALDNWWHFREYI
                     LFLLMLTVVAREPKKWERRILLGYLAGYCVSLLTSYLRWFEVIPLYDQVGEFAGFGGH
                     TGFSLMLAITSYACIWLWRARPGLRVFWLLLMILSLFNLYFINTGRTGQVAFLALIPL
                     IVFRWMGLPGLIAGALGAVAFAGSLYFLSPTVHYRVNNAVYQIEQYRAGNTTTSDGLR
                     IEFWVNAVKLIRHHPILGGGSGSFAAEYEVLAAEQHMTGDHVAVNPHNEYLLMWAQYG
                     LPGLGLFLALWLSQWRRACREAETRRYLFHGLILAMAVGDLFNSLILDNMEGHFYMLV
                     SVALGAAWARDDRAGGR"
     gene            45551..46477
                     /gene="ybiB"
                     /locus_tag="PKX01_01570"
     CDS             45551..46477
                     /gene="ybiB"
                     /locus_tag="PKX01_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HMZ74835.1"
                     /translation="MSYAPYIKEIGRGPKGSRPLTVDQAEQLFGDMLDGRVPDLELGA
                     IILSMRIKAESVDELFGFQRALDARTAHLAVPPGPRLVVLPTYNGARRQANLMPLLAR
                     LLAREGVPVLIQGRHDFESRVSPFELLAALDIHPATSLSDATDRLARDRLACVPLDLL
                     APGLVPLLATRVRLGVRNSAHTLAKLLDPAPGHSVRVASMTHPEYLERIHAHLVATGA
                     TAMLLRGTEGEAFANPRRRPLLERFANGVADVAFPAEEGGAPPLPGVTDNPEVAANAE
                     LIRAMLAGAVPIPSPLLDQVAALRDLAHLARA"
     gene            46606..47403
                     /locus_tag="PKX01_01575"
     CDS             46606..47403
                     /locus_tag="PKX01_01575"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ74836.1"
                     /translation="MSKKALCIGINDYPGTGSDLSGCVNDANDWATTLAGRGFTVTKL
                     LDAQATKAAMVSAMSSLIAGAEPNSTVVITYSGHGTWVPDQNGDEADGRDEGLCPWDI
                     AQGKVLIDDEIAILFAKRAKGVRVLLIADSCHSGSVTRAPEDIPEPGEPKPRFLPLSL
                     WLKTGYNPPVVPPKPPAGTLGLDRAGGDTLLAGCKDEEFSWDTSFAGRPNGAFTYYAL
                     KALGSLPANATYADWFKKIRTYLPSSACPQNPQIFGPDGADAFPIFS"
     gene            47467..52929
                     /locus_tag="PKX01_01580"
     CDS             47467..52929
                     /locus_tag="PKX01_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ74837.1"
                     /translation="MPRTYRIRGAQDASRPAVALPIADTAAISVTHVGHLLPGTARDA
                     APNELDIPADTVVRVTLDNGFVLWSRADDLIREYGRKALARDGGEGWEFGPLVPPRRG
                     AGERGLLGLGIKVLDFFGIHVMNKPAAAIAAWLEDKRLGGHAPGVYRCPLDGPFALSP
                     LGTKETLAAAPGPSLVFLHGTGSSTQGSFSELWDPKNASGAVLRARLKGVYGDRVYAL
                     EHRSLTESPIQNALQLVERLPEGTELHLVSHSRGGLIGELLCLSGVDALAESLSPTTL
                     DALFNPDRIIARQLGLGPLGEQALADRKAAYDAERRRLSDLVGLLAARKIRVSRFVRV
                     ACPARGTTLASGRLDRWLSTLDFLAGRVLGDGLFIGGLDFVLAVVHERTDPRTLPGLE
                     AMMPGSALTSLLAAPGLTTRADLTVIAGDIEGDSLLQNLKLMAVDWFYAADHDLVVNT
                     GSMSGGLKRPPGGARYRLDRGSDVNHFAYFQNKKSVEWLLAGLTRADGADGGFLTIAS
                     APAEAPRWRDALARSATVSAPRPLAVVLPGTMGSHLSVAGDHVWLNYGALLLGGLKKL
                     RRDANGVTPTELIDQFYGPLLEFLARSHRVEYVAYDWRLSIREAAGRLADTLERCLPQ
                     AERDNQPVHLVAHSMGGLVVRCMIADGARGASLWQRITRLPGSRFLMLGTPNFGSYEA
                     MRWLTGFNPTEAKLSLLDFTQSTDDIVNLVRRYPGLLELLPFGTQDPDFSTVAPWQSI
                     KDKTLAAWDVPQSDDLRIAAATWQCLRNAAPDPRFMRYVAGSQAATVTDYQLLADDDL
                     TNPRQHLDFIATRRGDGTVTWASGTLPGVPMWFVDDTPHDALCSATQAFPAYLEILAS
                     GTTNRLPSVADAVSRGDAPDERFPLPATPPEDGIPDESGLRGFGFGGGLPAETPNAAP
                     AAPVIEVSIRHGDLSYARFPVLVGHYQGDPLVHAEATLDRQLDGALSRRVNLDIYPGR
                     LDSHAVFFRENPRLKPTGALVIGLGQVGALTPGLLEQIATPALLDYALQAARWPDERF
                     GPASAPRSAAVSCLLVGSGAGGFPVRDSIEALLRAAVTVNRRLVDARLEGRITLDKLE
                     FLELFEDVAIGAAEALNQILADGEFAAAVSWPQRCIEPGQGGRRRIRFGDAPGWWHRL
                     DIARDGERLRFTFATDRARAEETLATGQLALADAFIRQASRSPRANPEAARTLFELLL
                     PLRLREMAPQQGDMVLLVDEDSARFPWELLEDRWSHSGRPPAVQAGLLRQLKTATFRA
                     RPSLGSEARALVIGNPDLSGSTVFSDLPGARDEATRVAGLLGTRGFETEDCIDAKATS
                     IVETLHKRPWRILHLAGHGVHRFMIAGDTPRPCSTCGQATPAPQKPISGMVIGDGIFL
                     TPGDVEQLRWVPELVFINCCHLGNTTAPADDLDRAGLAANLGVEFIRMGVRAVIAAGW
                     AVDDRAASTFAETFYGRMLAGESFGDAVRGAREQCWISHPGVNTWGAYQCYGDPAYRL
                     RPAVATTETGHTPFFAPSELVAELSNLIGQLKAHVDPDSPRIIEAILQRIPAAQGASW
                     RARADVSAALGLAWSEARCWPEAIAALEQAMQANSADLPVRAIEQWANLSARRAVECL
                     HGRAPITAAQRKEMVATLRTVIDRLQSLRTFGATIERLSLLGSTWKRLALVQTGSARV
                     AALGHMADAYGEAFALGKETDPYPFANAWTAELLQPDAPSRPERDTTCSRLLALQADA
                     NARNPSFWGDIAVADLKLARLLARCAGGGPAPRLPETCLELSQEVTEAYQAALARGAS
                     ERERASVTEHIDFLIRLTAPKSPQLAFLQTLRNTLA"
     gene            53145..55877
                     /locus_tag="PKX01_01585"
     CDS             53145..55877
                     /locus_tag="PKX01_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ74838.1"
                     /translation="MAETKSTCCYCGVGCGVIIEHDAGRITGVRGDPDHPANFGRLCI
                     KGSTLHLTAAPETLATRALHPELRTGRALPRQRVSWDTALDTAAERFAKIIAEHGPDA
                     VAVYGAGQLLTEDYYVFNKLVKGLIGTNNLDTNSRLCMSSAVAGYKLSLGADAPPCSY
                     EDIAHTSTLFITGSNTAFAHPIVFRRVEDARKANPDMKLVVIDPRRTDTAEGADLHLA
                     LLPGTDIALYNAMLHVMLWEGWVNRDYIDAHTEGFDALKRTVREYTPAMAATICGLDK
                     KVIEQAAELFATSPATLSMYCQGLNQFSFGTHNNSALINLHLATGQIGRPGAGPFSLT
                     GQPNAMGGREVGGLANLLSAHRDMANPQHRAEVAALWGVDSVPDKPGKSAVELFRALK
                     SGEVKAVWIACTNPAQSLPDQTLVHEALQSAEFVIVQEAFGHTETCAYADLLLPASTW
                     GEKDGTVTNSDRTISRVRPAVSAPGEARHDWAIVVDFARRLGTRLGKGDLAARMFPYD
                     RPEDVWNEHRESTRGRDLDITGLSYTTLEDVGPQQWPFIEGAQAGKVRLYADGVFPTP
                     SGKARFVATEYRVTAEAPSARFPLHLNTGRLRDQWHGMSRTGLVARLYSHESEPLLHM
                     HADDLARRQLSSGDLVRVKSRRGAIVVKVLESDTVRPGQCFLPMHWGANAMGGQGVNV
                     LMPPDFDPTSKQPELKHAAVQVEKFTEGKALVALRRSEPDANGVLQPNPLEVMESLRR
                     WLPHFPYASLTLAGRHEPVVVLKARGEELAPELLEALDRDLSLDDPTRVLSYQDRRKD
                     VAKRALVEGDSLTAVRLAGETRAADWLADMMVRGHSAAAVRPWLLAPVDQPPAGQSAR
                     GKVICNCFDVSEEDIVAAFKNGDTLDALQARTRCGTNCGSCLPELKRLQQQNSH"
     gene            complement(55947..56828)
                     /locus_tag="PKX01_01590"
     CDS             complement(55947..56828)
                     /locus_tag="PKX01_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HMZ74839.1"
                     /translation="MNWQDRALVFECGGESLVGILSAPQGPARVGVLVVVGGPQYRVG
                     SHRQFVSLARYLAARGVPCLRFDYRGMGDASGAARHFLDVSEDIRAAMDAFLAAQPGL
                     EKVVLWGLCDGASAVCVYPASDARVAGVVILNPWVQTSAGQAKVFLKHYYLQRLSDPS
                     FWKKLLGGGVNVFGSIRSLFSTARAARGDGAARTANSSDEADAGSLPERMAQGMLRRA
                     GTPAALFLSGRDYVAREFEQVRDGSERWQAVVTRPEVSTRHFEQADHTFSGPDDCPAV
                     SAATFEWLVAQGWAPAS"
     gene            complement(56825..57652)
                     /locus_tag="PKX01_01595"
     CDS             complement(56825..57652)
                     /locus_tag="PKX01_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HMZ74840.1"
                     /translation="MQPLRREAFFLPGAAHGGRFCLLTRAPEPVGSLVFVHAFAEEMN
                     KSRRMVALAARAFAERGWNVLQMDLSGCGDSGGELADVSWGTWIDDLRDAVRWATTDL
                     RVPVALWGMRLGALVVSDWIKADGTAPPLLLWQPVGNGKQHLTQFLRLKAAFDMLADA
                     DAKASMDAIRATLKAGGVVDVAGYRLPAALASGMEAATLSLPAAYAAPVVALEVAPAD
                     RQEVSPALRVPVDRWANAGTAARAAVVPGVAFWQTQEIEVCEPLIEASVEALKGFVR"
     gene            complement(57662..57910)
                     /locus_tag="PKX01_01600"
     CDS             complement(57662..57910)
                     /locus_tag="PKX01_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ74841.1"
                     /translation="MDTKKEVLSILDEVLSLGGRAAEFDLDTPLLGALPDLDSMAVVG
                     LINMLEERFGFVVEDDEIDGATFASVGSLVEFVDAKLA"
     gene            58082..59317
                     /locus_tag="PKX01_01605"
     CDS             58082..59317
                     /locus_tag="PKX01_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ74842.1"
                     /translation="MKRVLLIAFHFPPVQGSSGVQRTLRFARYLPDFGWEPIVLTAHP
                     RAYAETSDDQLKDIPPGVRVIRAPAWDSSRHLSIAGRYPGFLARPDRWMSWWLGAVPA
                     GLKIIREFNPLAIWSTYPIATAHRIGGSLARLSALPWIADFRDPMVDGDYPSDPAIRR
                     SFEAIQGNAIRRAACSTFTTPSALAMFRRQFPERANHMALLENGYDEETFAGQTATGR
                     LHPERLTLLHSGVIYPSERDPTQLFAALRLLKERAPAVLARLGVRFRAPIHFDLLADL
                     ARTYGVEEHVELAQPVPYREALAEMLNADGLLILQAANCNAQVPAKLYEYLRARRPVL
                     TLTDPAGDTAQVVRDAGIGALARLDDAADIASLLERFVAQPDTGTLPTEAAIRGASRR
                     GRTEALAGLLDRAVAHGGA"
     gene            59321..60013
                     /locus_tag="PKX01_01610"
     CDS             59321..60013
                     /locus_tag="PKX01_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ74843.1"
                     /translation="MALGKLRATFAEMGALNGGLYLLHRILQTLSGGRAQLVRYYLVA
                     QPIHPDAACRPSAKNPVTLLDPSDPLIAAFPRPPEVIAKRLRDGATCFIARSGETFAG
                     FLWLAHGAYDEDEVRCRYELVDDDRCVWDYDVYVEPAYRIGRTFARLWDAANQHLAPR
                     GVRWSISRISAFNAASLAAHDRLGIKILHSATFICLGSVQISALGGAPYLHIGMSDAS
                     RPVLRLRAPDAE"
     gene            60106..61710
                     /locus_tag="PKX01_01615"
     CDS             60106..61710
                     /locus_tag="PKX01_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HMZ74844.1"
                     /translation="MNATTLLHQLVTESALRAPDAGALRDGAAELSYEALAGQIGAFA
                     SGMMALGLDRTERVGVYLDKRFETVIASFGAAAAGGVFVPVNPILKPQQVGYILQDCN
                     VRVLVTSVERFKPLADTLSDCHDLRHVILTGKVDTLPELPGMSIHRWADVMAAPARAG
                     HRVIDNDMLAILYTSGSTGRPKGVVLSHRNMVAGAKSVATYLENRADDTLLAALPLSF
                     DAGFSQLTTAFHAGARVVLLNYLMPRDVLNALVKHKVTGLTAVPPLWIQLSQLEWPET
                     VTDHLRYIANTGGRMPLETLTALRARLPRTKPYLMYGLTEAFRATYLPPDQVDVRPDS
                     IGKAIPNSDVFVLREDGSPCEPGEPGELVQRGALVAMGYWNDPEKTAERFKPLPVTAP
                     GRQAGLVLPEIAVFSGDNVRRDEEGYLYFIGRRDEMMKTSGYRVSPTEVEEILYGTHL
                     VGECVAFGVPHPSLGQAICVIATPRAGSALDSAALLAECKQRMPAYMVPSVIEVRDGP
                     LPRNPNGKIDRKTLSTEFENARKVQS"
     gene            61707..62951
                     /locus_tag="PKX01_01620"
     CDS             61707..62951
                     /locus_tag="PKX01_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HMZ74845.1"
                     /translation="MSDTPREKPVHAPMDQFPVVNGELTVGGISLTRLADRVGQTPFY
                     AYDRALLTRRVQDLRATLPTAIKLHYAMKANPMPAVVDHMARLVDGLDVASGRELRVA
                     LDSGANPHEISFAGPGKSHQELRQAVAAGILINIESFREVAELAAIRQATGWHVRVAV
                     RVNPDFELKSSGMKMGGGPKQFGVDAEQVPELLAEIGRSGLAFEGFHLFAGSQNLRAE
                     SIVEAQRKCFDLALRLAESAPSPVRFLNLGGGFGIPYFPGEQRLDLAPIAANLAIIAE
                     EAAVKLPGAELVIELGRYFVGEAGLYVCKVVDRKVSRGHTYLVTDGGLHHHLSASGNF
                     GQVIRKNYPAAVGNRMDAPLGEAVSVVGPLCTPLDLLADRMALPEARPGDLIALYQSG
                     AYGATASPRGFLSHPDLVEVLV"
     gene            63194..64105
                     /locus_tag="PKX01_01625"
     CDS             63194..64105
                     /locus_tag="PKX01_01625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMZ74846.1"
                     /translation="MDATDASLPAPLITTIIPTMASKARAASLKRAITSIRAASKQPV
                     AIIVVVNGTRSDPEACAWLADQPDVRLEFDAKPSAANAMLRGRELVATPFFTSLDDDD
                     EFTPGALDLRLAAMQSDTCPDIVVCNGLRRDSTGDTLCYDDLPSVPAAPLPALFRMPW
                     LSSCNSLYRTASIDAHFFRNFHSFAEWTWLAYQFGVAGKKIAVIDTPTFVCNDTAGSL
                     SKSNEYEMAYLSLYERMLSLAPPAPVADLIRRRIGSFWHDQSVTALRNGQRLDALRHH
                     VRSLRQPGGLRYLGYSRYLIPGWPQNR"
     gene            64102..65586
                     /locus_tag="PKX01_01630"
     CDS             64102..65586
                     /locus_tag="PKX01_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HMZ74847.1"
                     /translation="MTDSSSNSVFKGALMTVLMRWTDRLIGFISTLILARVLVPEDFG
                     VIAMASLVVGLLDVLLDLGVNVALIQNRTPSQAHYDSAWTLRLAQAATTALLIVIASP
                     FAGDYFNDPRVTPVLQFMALSLFVAGLENIGTVTFQKDMRFDLEYRFNFSKRIVAFLV
                     TVTSAWLLHSYWALVFGTLSGRIFGVFLSYRMVAMRPRLSTEKMREIFAVSQWMLVNS
                     IGIYLNANLHKMFVGRFASTGTMGGYTLADEISAMPSTEILAPLNRVLFPAFVRAKHD
                     LVELKRVFLLAQGVQCLIAVPASLGLAFIAHDAVLVLLGERWSLAIPFVQVLALSNVV
                     QAITNSGAYMLLTLGKVRSVTLTTWAQVILVAIGALLVLHQPEALDIAILRVSAVFAG
                     LVLAMWILMLNMSGLRLLELVRTIVRPILATAVMGGAIHGLGVLTADLPVVVAMFLKI
                     FAGMFSYTAAVVLIWRLAGRPDGAESYVFDKLAGVLRRRKAGTA"
     gene            65632..67446
                     /locus_tag="PKX01_01635"
     CDS             65632..67446
                     /locus_tag="PKX01_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMZ74848.1"
                     /translation="MDNRFSIQGRLSPNPAGTPGELSIERLDDGWRCLWAGARLEVHA
                     PARLAERDGILCVASGSPRLPVSGADIADAWLELHARHGADVHRDVKGLFSVAFFDTR
                     RQTASFYTDRFATESWCFGEEDGALCFSDRADAVPLTRRSVSAQSIFDYLFFHVIPSP
                     VTLFREVRRLPPASRLDWQNGSTRQAPYWVPVFREDTRVSVEDAKARFMEIVRDSVTR
                     EIDRDDVGAFLSGGTDSSTVSGMLGRITGRPARTYSMGFDATGYDEMEYARIAAKHFK
                     TDHHEYYVTPEDLLEGIPKVARSYDQPFGNSSAVPAWICASRARADGIGKLLAGDGGD
                     ELFGGNARYAKQRVFGWYDTIPSPLRSGLIEPLSGLPGMDRIPLIKKGVSYVEQASVP
                     MPDRMQMYNLLLRLGLDTVFQPNFLKGIDTGEPSRLQRDTWALVPEGSLLNRMLAFDW
                     KYTLADNDLPKVIGTTRLAGVDVGFPLLSDELLDFSLTLPSEWKLKGFKLRWLFKEAL
                     RGFLPDEIITKKKHGFGLPFGVWAVKHPGLKALACDTLEAFGQRGVMSPTFLHDLHAK
                     WLPEHPGYYGELVWIVMMLEQWLQAYAPDWRLGGPARA"
     gene            67443..69671
                     /locus_tag="PKX01_01640"
     CDS             67443..69671
                     /locus_tag="PKX01_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HMZ74849.1"
                     /translation="MTAARPDAPSAASASRPPARAGAALLRLGLPLLLIALVWLAHYA
                     HFHVDRSPVAEAVVEQARQAPDDARLDEIGHMSLAIDLGVPSDRVVATADLIVRGTAL
                     FPAYLDGPTPLTGYPADFRFGPGTFQLAAAGFGFEDILLEAYERTADARYLRTAYQRI
                     QDFAAFELTQREAVDFLWNDHAIACRVAVVARLWRHIRHDPAYPADGLRNLLSLIARG
                     GRFLAAPDHFTARTNHGVIQNVALMQIAAAFPSLPDSAGWARLGMERLALQLPFYVSP
                     EGVVLEHSADYQHVGVSLLAMAVRLARLNDLPVSRELLDKSAASRTVQSQLQRPDGSL
                     PAVGDSIVGRPHTAVTGATDGSTPVAEQPAPFPLPEPAAHVYPVSGYALWWTGDDPAL
                     RSQTVIAWAKHDGHGHKHADEGSLLLWSGGRDWLTSIAYWPYGGANEKAAYGWSGANA
                     PHGIGELAASVRTVELLGSADDAQMHAVDLLRRTGDGAQFRRQIVQVDAQTWLVLDFV
                     DGSASGSETDWTLPPALGLTARTDDRSFQSTEAGGRRLILSLAAPSTTRSRILRGSQD
                     PFAGWVSVNLRPTPASTLRVTDPSARSVTALLLALRPADRIGPQLSVAPGATPEAWSI
                     HIDEEGSRHAVTRQGARLAMDDASVLSLAAPADIAPAQAVLRAAYRKAVDAFPPWRDL
                     SRFRARIAYGLLALMVAQEIAWQVFIRRPATTPGQRRGVLLGLSAGWIALGAWIFGIY
                     LA"
     gene            complement(69857..>71433)
                     /locus_tag="PKX01_01645"
     CDS             complement(69857..>71433)
                     /locus_tag="PKX01_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74850.1"
                     /translation="LRCRRRCARRGRDEGRRVIRLLGRMLAVGASVLTLGAWAASPEN
                     SLPDAIDGASCHYFNGVGGGRWNREGGDWADADRRSYGTRAYSTTVIREGSRGHLVEI
                     DMTGLARDWLAGKADPAQGAMLMSTSGPGGNIVVFASREHPDITARPLLRLEFKDGRR
                     TAFDPVSDAYLDCSTEKGLGFEPRLRAGGTYRSLLAFALPVEKFAGRVLDKAVLTMRV
                     DDQYGNDTTLGVFAVRAPESPDRLPRPGLAAAYVGDAGIAKDPRVLFFSDFEADRWSS
                     AWSEYDSRSTSTTVTSDPERLFLPLSGRALRVTVPRNGNLGLDLRYNYRDKTGSEPEE
                     AYFRYYLRFAADWGPEQSAGKMPGFAGTYGKAGWGLRKSTGDDGWSLRGQFSMVSRSG
                     NPLSGRTGMGTYAYHAAMRDPSGDFWPWPLGRLAALANNRWYCVEQYLKLNTPGKSDG
                     ILRAWIDGRLVVERTDIQLRNVPQLRIESVWFNVYYGGVDPSPRDMSLYIDNVVVATS
                     YIGPMGRSSEAGAPVR"
BASE COUNT        12267 a        26287 c        21786 g        11093 t
ORIGIN      
        1 cacgtccacc gccgtctcca gcggccggcc gaggagtcgg ttgtaggccg ccgcggcgag
       61 atccacgccg tgccaggcgc gcgtctgctg ctgacgggca tcggccagcg cgacggccac
      121 cgacagcacg tcgttgcgcg gcaccacgcc ctggcgatgg agggcattca cgtccgccgc
      181 gtgggcgtcg agggccgcca cgtggcgctc cgccgccgcc agcgcgcgcc ggctgcgcaa
      241 cacgttaagc caggcctcgg ccacgtccag cttgaccgtc tgctcggtgc gcagacgttc
      301 ctgggcgctg gcctgcacgc cgatgtcggc cgccgccacc gccgccggga tgcgtccgcc
      361 cgtatagatc ggcaggctcg cctgcagggc atgggtggcg taggtgcgct cggacagggg
      421 catgcggtcc ggcaggctga tgccggggag ggtcggcagg ccgatgaggg ccgcgggctc
      481 cttgctcagc acggtgtagc gggaactcgc cgtggccttg ggcagggcca ggcccttcgc
      541 ggcctccagc gtggcgcggg cgccctcgcc ttcggcatcg gcggcgcgca gacgcgcatc
      601 taccgacaag gcctgggccc acgcgtcggc gagggtctcg gcggcccaac ctgtcggcgc
      661 cgcggacagg agcaggaccg acagggcgat ggaaagcgga cgaatacgca tcgggaaggc
      721 cgcccgaccg cgtgtttggc cagcggatga cgtcgcgtcg aagtgcctct tcatcacgac
      781 ctccaattac tgactcatcg gtcactaaac tactccgccc cgcacgcggc agccttgcgg
      841 aaagtcaacg gagcgcacgg cctcgcccgc gacgacgccc gtgtcggaag cattccgccc
      901 atttcccgga acggggacga tgcagcacat cgcgggcttc gcacagcgca aagaggctgc
      961 acccgctgca tcgcccgcct ccctgacatc gtcacccagc cgccaggacc tggccccgga
     1021 tttgcatagg cctgctgatc acgcccactc ccaggaacgc tccatgcccc ttcccgccct
     1081 cgacgacgac ctcgtggact tcggtgacat ccccgcgccc atcaacgccc tcctgcagga
     1141 aggcgtgtat tggcaccccg ccgatccggc ccgcgcccgc gcctgtttcc gcagcgccat
     1201 cgacctggcg ccggacgtgc tgccgtccta ccgctgcctc ttcaaggccc acaacaagca
     1261 gcgggacttc gccgccgcct acgccgtggc cctggaaggc ctgggccgcg cggccatgca
     1321 ggccggcatg agcccggact ggagcacctg gacgcgcccc gccctggccg ccgcgccgac
     1381 ggtgccggcg cgctttctgc tgcattccct caaggcggtg gccttcattg aactgcgtcg
     1441 gggccacgaa tcggcctccc gcgcagccct cgcccatctc caggcgctgg acccggaaga
     1501 cggcgtcggc gcctcggtga tcgccgccct cctcgaccgg gaagacgcct gatgcgtcgc
     1561 gaatccgaca cgccgccctt tgcggtgacg caaaccgtac atcgcgccct cggcccgctc
     1621 gtggtgctgg aggtgggcgc gctgaaggtc cgcctcacgc cgcgggaggc ccataccctg
     1681 gcgctggccc tcttcgccgt gcgggatggg cgcagcgccg agaccgagct gttcatgagc
     1741 cccatcgcca gcgacggcat cttcgtcgcg ccggtggggt cggacggcat cgccgtcgag
     1801 acgtccgccg gaccggtggc gctggactgg caggcggtgg gcgagctggg tgcccagctg
     1861 tcgcccgcgg cagtgccggg ctgaggcgct ccggctacaa gacgaccgca gcggcggcgg
     1921 cgttttgccc ttggccgaac ccatgcagcg ggcgtggcca gcgcccggcg cccgggtttg
     1981 cgcggttcgc ccccaaggat catcgggaat gccggacggc tgtggtattt ttgcggcctt
     2041 taccgcccac cgggccgcct gaacaccccg tcaccgcgcc atgaacgaca cgccggatct
     2101 cttcgacacc ctgcctcccg acacgcccga accgccaccg ccgcccgtgg ccacgccgct
     2161 ggccggcagt gacgacgccc tgcccctcga cctctacgcc gagcgcgcct acctctccta
     2221 cgccatgagc gtggtgaagt cccgcgccct gccccaggtg gaggacggcc tcaagccagt
     2281 gcagcggcgc attctcttcg cgatgaacga gatgcgcctg tcgcccgcgt ccaagcacgt
     2341 caagtcggcg cgcgtcgtgg gcgacgtgat cggcaagtac cacccccacg gcgactcgag
     2401 cgtgtacgac gccatggtcc gcgtcgccca ggatttctcc ctgcgctacc cgctggtgga
     2461 tggccagggc aacttcggct cccgcgacgg cgactccgcg gcggccatgc gctacaccga
     2521 gtgccgcctc acccccatcg ccgagctgct gctgtcggag atcgaccggg gcaccgtgga
     2581 tttccgtccc aactacgacg gcgccttcca ggaaccccag ctcctgccgg cgcgcctgcc
     2641 cttcgtgctg ctcaacggcg cctccggcat cgccgtgggc atggccaccg agatcccgtc
     2701 ccacaacctg cgggaagtgg cccgcgccgc gattcacgcc atcgagcacc cggaggccac
     2761 cgtcgccgac ctgctcgggc acatcgccgg gccggacttc cccggcggcg gacagctcat
     2821 ctcgccggcg gcggacatcc gccaggccta cgagaccggc cgcggcagcc tcaagctgcg
     2881 cgcccgctgg atcgtcgagg acctcgcccg cggtcagtgg cagctggtga tcaccgagct
     2941 gccccacggc gtgtcggccc agaaaatcct cttcgagatc gagacgctga ccaatcccca
     3001 gcccaagacc ggcaagaagt ccatcgaccc ggatcaggcc cagctcaagg ccctgattct
     3061 gtcggccctc gaccgggtgc gcgacgagtc gggcaaggac gcgccggtgc gcctggtctt
     3121 cgagcccaag agccgcaatc aggacgtggc ggagttcgcc aacctgctcc tcgcccacac
     3181 cagcctggaa acctcggtcg ccctcaatct ggtgatgatc ggcctggacg ggcggccggc
     3241 ccagaagaat ctggccgaca tcctgcacga gtggggccgc ttccgcaccg tgacggtccg
     3301 ccgccgcagc gtccatcgcc tgggccaggt ggatgaccgc atccacatcc tggaaggccg
     3361 ccacatcgtc ttcctcaaca tcgacgaggt gatccgcctg atccgcgagt cggacgatcc
     3421 caaggccgcc ctgatcgccc gcttcgacct gagcgaccgc caggcggagg acatcctgga
     3481 gatccgcctg cgccagctgg ccaagctgga aggcatcaag atcgaacggg aactggccga
     3541 gctgcgcggc gaaaagaccg agctggaagc cctcctcgcc gacgagggca agctgcgccg
     3601 gctggtgatc aaggaaatcc gggccgacgc cgagaaatac ggcgacgcgc gccgcaccct
     3661 ggtggaggaa gccgcccgtg ccggcgtcac ccagaccgtg gtggacgaac cggttaccgt
     3721 catcgtctcg gaaaaaggct gggtgcgcgc ccgcagcggt cacggcctgg atctggccaa
     3781 tgtggccttc aaggatggcg accgccttgc caccgcgctg gaatgccgca cggtggatgc
     3841 gctgatcgcc ctcacggacg gcggccgcgc cctgacggtg gcggtgtcga cgctgccgga
     3901 cgggcgcggg atgggcgccc cgctggcgtc atttgtcgag ttgccggcgg gggcgaagat
     3961 cgttcacgtg ctgagcggcg atccggccag ccgggtgctc atggcgaccc gcgacggcta
     4021 cggtttcctg tgccgcctgg gcgacatgct ggccaccaag aaggccggca agcagttcgt
     4081 cacgctggac gagggcgtgg gactattgcc accgttgatc tacgccgacg cgccggacct
     4141 gcacgtggcc accctgtcgg agaagggtcg cctgctggtc ttcacgctgg accaggtgaa
     4201 ggagctctcg ggcggcggcc gcggcgtgat cctccaggcc ctggaagacg gcgacaccct
     4261 gcgcgcggtg tgcgtggccg gcagcgacgg catcgtgctg tccagcgcct ccagcgccgg
     4321 gcgggcctat gaggtgccca tccccgcccg cgactggacg ccctggctca acaagcgcgc
     4381 ccgcaagggc aagcccgcgc cgggacgctt cacgcccacg gcggtgacgc ccctcaagcg
     4441 ggactgagga ccttcgccgc ccagaacagg gcggtaatgg tcttggcgtc ggtgatgcgg
     4501 ccgtccagca cggccgcttc ggcctcgtcc agggacatcg ggaagatctc cagatattcc
     4561 ccatcatcca gcgacgcgcc ttcgtggcgc agcccgctgg ccagaaagat ctcgatgcgc
     4621 tcgtcggaat agccgatgca cggatgcatc acgcccagat ggcgccagga gtcggccacg
     4681 tagccggtct cttccagcag ctcacgccgg gcgcagcgtt cgatgtcctc cccggcgtcg
     4741 atcttgcccg ccggaagttc gatgaacacc cgcccgcagg gatagcggaa ctggcgctcg
     4801 aagatcagat ggccgtcggg caactgggcg atcaccacca ctgcgccggg atggcggatg
     4861 tattcgcgga caccctgctt gccgtcgggc aggcgcaccg tgtcccggtg aacgttgagg
     4921 agaacaccgt cgaacacctg ctcggacctc acgcagattt cggtcaactc tgctgccatc
     4981 acacactcca tgaaaaaagc ccggcgggac agagccgccg ggcttcgggg tccacacgca
     5041 ttcgcgcctg ctcccgggga agccggcgcg aaccgtcaga cctgcatcag atgcgctcga
     5101 acacggtggc gataccctga ccgccgccga tgcacatggt caccagcgcg taacggccgc
     5161 ccgtgcgttg cagctcgtac agcgccttgg tggcgatgaa ggcgccggaa cagccgaccg
     5221 ggtgaccgag ggcgatggcg ccgccgttcg ggttggtctt gaccggatcc agacccagca
     5281 ccttcatgac ggtcagggcc tgggcggcga aggcttcgtt ggactcgatg acgtccatct
     5341 ggtccagggt cagaccggcc ttcttgaggg ccagcttgga ggccgggatc ggaccttcac
     5401 ccatcacgtc gttgggtaca ccggcgatgg cgtaggaaac caagcgggcc attggcttgt
     5461 agccggcgtt caccgcggtg gcgccatcgg ccagcacgaa gaaggcggcg ccgtcgttga
     5521 tgccggaggc gttgccggcg gtgacggtac cgtccttctt gaacgccggc ttcatcttgg
     5581 ccagggattc cagcgtggtg ccgcgcttga cgtgttcgtc ggtgtcgaac accacgtcgc
     5641 ccttgcgggt ctgcttcacg atgggaacga tctgggactt gaagcggcct tcgtcgatgg
     5701 ccacagcagc acggcgctgg gattcgacgg cgaaggcgtc ctgctcttca cggctgatgc
     5761 cgtgcttgac agccaggttt tcagcggtga tacccatgtg gccgacaccg aacgggtcag
     5821 tcagcacggc caccatgctg tcgatggcct tggtgtcacc catgcgcgca ccggagcgca
     5881 gcgccggcag catgtaggtg gccttggaca tgacttcgac gccgccaccg ataccgtaat
     5941 cggcatcgcc cagcaggatg ttctgggcgg tggtgacgat gccctgcaga ccggaggagc
     6001 acaggcggct cacctgcatg gccacggact ccatcggcag gccggcctgg atggacgcca
     6061 cgcgggacac gtaggcgtaa cgggcgtcgg taggaatgca gttgccgacg gtgacatagt
     6121 tgatcaactg gggatccacg ccggaacgaa ccaccgattc cttcatcacg ataccggcca
     6181 gctcgctggc gtcgaaatcg gcaagggaac caccaaacgc gccgattgcg gaacgaacgg
     6241 cgctcagaac cactacatca cgactagcca tctagtaacc ctcctcttat gagaccatca
     6301 atgaccaacc caacccgcca cacccaagag cgccagtacc agaaatacca gcaccagggt
     6361 gcgccagatc aggccgatcg tgctttgcat gaaatcggca tcagcctcgt cgccaatgcc
     6421 catctcggga cgctcgacaa tttcgcccga ctcgtggacg ggcatgccga gccgtacgcc
     6481 cagcgcccca cccccactcg cgagcagtat accggaagcc ttgtctgccc attgggcagc
     6541 ctgggctcgc cagcaataca ccgcatcttc gaagtctccg acgatggaaa acgccgccgc
     6601 ggacaaacgc accggcaacc agtccagggc cgcgaacgcg cgatgggcga attcaccgaa
     6661 atcgccgaac tccggctcac ccttgcgcag actccattcc tcgtcgaaga agcgggccag
     6721 ccggtagagc aatgcgccgc tcggcccggg cagcaggata aaccagaaga tcaccgcaaa
     6781 cacattgcgg tgtgccgcaa tcagcgcctc ctcgatcgcc agcctggcca cctcggtgga
     6841 gctcgatgca tcatgactgc gcttgcgcca gacgccgatc agttgccggg cgcgcgccag
     6901 ctcacccatc cgcagggcca gttgaagatc ggaaaagaaa cggctcaggt tgcggaacgc
     6961 catggtgaaa tagaggatca ccacgttgaa gaccatcgcc agcaacggat ggacgtgcca
     7021 caacacaaaa taggccagcg ccgacacgaa tcccacgctc agcatggcca cccaccaggc
     7081 gaccgccccg tgacgcgcct cgccgtcgtt gaagcggtct tcgagaaagt gggcgaacgc
     7141 cttgagaggc gcctccacga accgttcggt ggaaatgggc cggagatgct cgatgagcag
     7201 ggcaacgatc agggaaaaca gggtcatgcg cagtggggcc ttggtcagcc ctcgacaaac
     7261 tggcctattg gggaagcgct aagataccac cattccacga accccggaac ggcggccgat
     7321 caggcactga gaaagcgctg caggctcacc agcatgcccg ccgtggcacc ccagatgtaa
     7381 tagttgccgt agggcatcgc gtaatactcc cgcagcgctc cacgcaccac gacccgatgc
     7441 cgctgatggt tggcggaatc gagcaggaac gccaggggta cctcgaacac ctctgccact
     7501 tcgaaatcat ccaccttcag gtcgaagggc ggcgcaacca gccccaccac cggtgtgact
     7561 tcaaaacccg tccccgtgcg gtacaccggc agatgcccga gcaattccac gtgctgacgc
     7621 tgcagaccga tctcctcctc cgtttcccgc agggcggtct cgatcggtga ggcatcatgt
     7681 tcctccatcc gtcctcccgg aaagctcacc tgtccgggat ggtggtggag atgatccgtg
     7741 cgacgcgtca gaagcaccgt cggcgtgtcg cgcatgacga gcggtacgag gacggcggcc
     7801 gggcgcaatt cctccggcac gccctcttcc acgggcgtct cgatcgcggg agattcggca
     7861 aacctgcggc gcaactgatc cgctgtggga cacgatagac tcatgaggtc acgcacgacg
     7921 ctcagtaccg ctgcttcagg gtcatgacgc cgttgtcgcg ccgcatttcc atccggttga
     7981 ggaagctcat gcccagaagc gcgacaggaa gatcctcacc atggatgctg gcccgcacgt
     8041 ttctttgagt cacgttgccg acacgcacgg aatccagcat cacaatccag gttcttgcca
     8101 cgccgtttgc cgtaatggac tgggccggtt cgcccttctt gtagtcgatg ccggcccgta
     8161 tggcatcgct acgtcccagc acgatcagac tcgcaccggt atcgaccaga aaattcacct
     8221 gagccccatt caccgtaccg taagtaagga agtgaccggc acgatcggcc tgaagttcca
     8281 ccgttccgcc gccggatgtc tctcccccca cacgcacggc cgactgcccc accaccagcc
     8341 gctggcgacg cccgtccacc tccacggtcg cggtatcacc ttccaccgtg atgacacgca
     8401 cgccttccgg cgtcatggcg cccgatgcaa ccgcccgcgg cgcacggcca tcgatcacca
     8461 gcagcgcctt tcccggaaag acgcccgcca gcacgacatc cgtcgcctgc gccaggtcca
     8521 tcgccgccat ggccaaggca gccaggacgc cggacgccca cctctgtgat attgaaaacg
     8581 cttgaaagcc cgccacgtcc cgccacccgt ataccgacac ccgtcattct gccatggggc
     8641 gcgcaccatg cgccctcggg ggacccgcgt cagggcacca gaagggcttt cagatagtga
     8701 gtcgggatgg cataggagat gcccgttggc attgccacgg cactttcctt ggtgcccttg
     8761 acgaacacca tattcaggat gcccacaacc tccccggagt gggtatcgaa gagcggactg
     8821 ccactgttac cgggatagga caccagatcg agctgaaaca cattgtaggc ccccatcgcc
     8881 agacgcctga ccgtacgggc atcgagttgg ctcgccgacg gtgccggcat gccgacgggt
     8941 gtcaccgcgg agacgatgcc gcgatgggtg accggcacga gccccaggga agcgcccagc
     9001 gggaacccgg tgatggccac ctcctgaccg tcccggaccg cgcccgaatc gcccagcctc
     9061 agcgccggca gcggcggccc gtcaaagcgc agcagcacga gatcgtgatc ggcatcggac
     9121 gcctgacgca ccactgcacg cccgatcaca cgccccgtgc cgggcagggc caccgtcaag
     9181 gtttcgtttt cttccagggc gtcggacacc acgtggatat tggtggcgac gagattgcca
     9241 ttacccacga caaatccggt tcccctgaac ttgaactggg gattacggga cgggaggaat
     9301 gtgccaaccg ccaccacgcc gttcttgatc tgggcaacga catccggaaa gtcggcccat
     9361 gtgggcatcg cccatgttgt ggccaatgca cccaagccgg ccaacacccg tctgcgctgc
     9421 gccagtccgg ccatgttcaa tccttgaatc gggtcacggc ccggaaggtc tgcacgatag
     9481 gcgacgcgtc caccagccgc gcgtcgaagg gatgatcggt gcggggaaag aacgccagga
     9541 tacgccggac gtagttctgc gtttccttga acggcggcac gccgcgatag cgatcaacag
     9601 ccccttcgcc cgcattgtag ccggcgaccg ccaaggccac atcgccccgg taataagcca
     9661 gcagccagcg caggtaggcg aggccgccct tgatattctg taccgggtcg aaactgtcac
     9721 ggacgttgaa gcggctcgcc gtttccggaa tgagctgcat cacgcccatc gcattcttgg
     9781 gcgagcgtgc gccggtgtcg aaattggact cggtgctggc gatcgccaac gccagccgcg
     9841 gctcgatgga gaagcgaggc gccagggtgg tgagaatctc caccactttt ttcttctggt
     9901 caggaagcga ggcaaggtat ttctgggtat cccagctgtc ccgagcggcg aaaccgtggt
     9961 cggccgggcg atccttgagg cagtccggca ccgcgccagt gtagtcgctg gcaaagcgcc
    10021 gagccctgag cgctccgtca tggcccttga cgatggccat gtcgaagagg gtcgtggcga
    10081 ccgcgtcgtc ccgcaggacg ccgcgcccgt tgagatacat ccaacccagg ctgtacattg
    10141 cttccgggtc gccggcccgc gccgcctcgc agtacagcac ggcagcccgt tcgagatcgc
    10201 ggggaacacc ctccccgtgt tcgaaggcca cagcctgctc gacaagcatc cgggccccgt
    10261 cgacacctga cgggagcacc gcgtccccgt ccgccacggc agcctgcccg acgctcgcac
    10321 ccgtcaggca gatcaccgca ccgaccgccc gcaggcagcg gaggagactt acagccgcca
    10381 cacgctcagc ccggccgcct gcagctgggc cggatcgtag aagctcttca cgtcggtgaa
    10441 cacgccgccg gacacgattt tctcggccag cgcgcccaca cccatctcgg catactcctt
    10501 gtgggccacc gcggccacca tggcgccggc cttgggcagc ttgtcccagg acgtgaggct
    10561 cacgccgtat tcgtgctcgg cttcggcgga ttcggccacc gggtcgtgga cgtgcacgtt
    10621 gcaaccgaag ctctgcagtt cgcggatcac gtcaatgacc ttgctgttgc gcaggtcagg
    10681 gcagttttcc ttgaaggtca ggcccagcac gatgacgtcg gcgcccttga tgttctggcc
    10741 gctgcggatc atctgcttga cggtctcttc ggccacgtac ttgcccatgc tgtcgttgat
    10801 gcgacggcca gccaggatga cttgcgggtg gtagcccagc atgtcggcct tgtgggtcag
    10861 gtagtagggg tcgacgccga tgcagtggcc ccccaccaga cccggacgga agggcaggaa
    10921 gttccacttg gtgccggcgg cctggagcac ttcgagggtg tcgatgccga tcttgtggaa
    10981 gatgaccgag agttcgttca tcagggcgat gttgaggtcg cgctgggtgt tctcgatgac
    11041 cttggcggct tcggcgacct tgatggagct ggccggataa acgccggcaa cgatcacggc
    11101 ggcgtaggtg tcagcgacga tcttgagggt ttcgggggtg tcaccggaga cgaccttcat
    11161 gatcttggtg acggtgcgtt ccttgtcgcc cgggttgatg cgctcggggg agtagccgac
    11221 gttgaagtcc tgcttccact tgaggccgga gaacttctcg aggatgggga tgcacacttc
    11281 ttcggtggcg ccggggtaca cggtggattc gtacaccacg atggcgccct tcttcatgtg
    11341 cttgccgacg ctctcggagg acttgaccag cggcgtgaag tccggctggt gggcgtcatc
    11401 cacgggggtg ggtacggcaa cgatgatgaa gtcagcttca ccgatgatct tgggatccgt
    11461 gctgcaggtg aggtgcacgg ccgcctgaag gtcgtcggtg ctgacttcgc cggtagggtc
    11521 aacgaagcgg ccgtaagccg ccaccttttc ggcggagagg tcgaagccga cagttttcat
    11581 cttcttgccg aattccacgg caaggggcag gccaacgtag ccgagaccga taacggcgat
    11641 ggtggtcata gagagtttcc tgatgaagtt tcagataaat tgaatcggat caaagggtct
    11701 tgcgcagccg ttcgagttcc gccttttgcg gggaattctc gggtgcggtc ttcaacgcgg
    11761 cctcgatttc ggtgcgggcc tcgtccttct tgcccgcctt gatgagcccg ctgaccagac
    11821 tgacctgcaa ttgcgggagc ttgggcccca gactgactgc cttacgcagg ttctggatgc
    11881 ccttttcggc atctccaaac tgaatctgaa gcgtgccgag cgtgtcgatc agcacaggac
    11941 tgttgggcgt catggccagg gccttgtcga tgacctgcag agcacccttg tcaccctgtt
    12001 tgcccatggc ccaggccagg ttgttcaaga gcaggggatt cttcggcgcg atgtccacca
    12061 tctggcgata ctgctgggcg gcctgatcga acttttgctc cttcaggctc cgttccgcca
    12121 gatacgcacg gaagatcaga tccttgggat tccccttcag ccagtcagtg gcgagcttcg
    12181 cagcctcggc cgactggccg gccgccagac gggccacatg gagccagatg caggactggg
    12241 cgctgtggtc aagcctgaat gcttcctcgt aggcggtgac cgcctccgtc ggcttgccgg
    12301 cggcggtata ggcctcacct tccagcacgt atccgacggc ggccttcccg ttggccttct
    12361 ggatgtcccg cgccacgcgc cgcgcgtcgt cgaaacgctt gccggtcatc agcactgcaa
    12421 acagacgctg ctgggcgtcg acactgtcgg gcttgacggc caaggccttg cgcagactct
    12481 gctcggcgcc ggacaggtcc ttggaggcaa tctgcacatt ggccatcgcg atcagcggcc
    12541 ccggagaggt gggcagcttg gcagccagtt tgccgaaggt ggtcagggcc tgctgcagct
    12601 cgcccgcagc cacctgggcg cgaccgagca gttcaagcac ggctgcgtcg tccgccgccg
    12661 ccgccgccgc ctcctgggcc aacgacagtg cacgcttcgt gtcgcccccc tggacaagca
    12721 tgcgaaccag cgccagacgc agcggcaatg cggtcggcac ggccgtcacg ccccgctcca
    12781 ggatcgcctg aacatccttg ggctggctcc cggtctgggc cgtgaattcg gcaaactgga
    12841 ggtaaccgtt gggatccttc ggtgcccggt tgaggaattc ctcgtatcgc ttgcgcgcac
    12901 gttcgagttg cttgtcggca acgtcgaggc gcgccagatt ggtcaccgcg gcgagaaagt
    12961 ccggtttgat ggagagtgcc ttctcgaagg cttcccgagc ccccggaatg tccttgttgg
    13021 ccagcagcac cccgcccttc atgttgaagg tcaccgggtc gttgggcttc tttttctcca
    13081 gcaccgccac ggcggcaagc gctttcgcca cctcgttgcg acgcaagtgc gccatgatca
    13141 aggtaacgtc ggcctggatg ttgtccggat cgagggccga cgcggcctcc agatcctgga
    13201 aggcccgatc ggtctcgccg gcggcgagac gggaagcgcc caggcgggta cgcagctggg
    13261 cattcttggg atccgacgcc gcggcacggc cgaagtattc ctcggaacgg gcaaagtcgc
    13321 ccgccatggc atagacctga ccggccagat tgaggatcgt gacgtcttcg gtacgatcct
    13381 tgatcagcgg ctgcagcgtc tcgagcgcct tcgaaggctc ccgcagaccg acgtaggccg
    13441 acacaagcag ccggcgcgcg agggtggcac ccgggaagcg atccacgatc ttggccagat
    13501 tggcctgcgc ctcgacgaat tcgctgcgct gcaactgcag cgtcgccatc aacatcagcg
    13561 ccggcacata gtcgggcgcc acgcgcagca cctgctggag ctcgtcgtag gcttccttca
    13621 tcttgccctg ccgcgtatcg agaaaggcct gcaggtagac cgccatcggg tgattgccca
    13681 gcgccttgcg catggcggca atcttggcgc cggcgtcgtc cagcttgttc tgccgcagca
    13741 gcagggacac gacattctga tgcgcgatca cgtccgtcgg acggactgcc accagggctt
    13801 cgaacgcctt gagcgcgtcg tcgacacgat tgagcgcgag catcagatcg ccccgcaggc
    13861 tgagagcctc cggcagattc ggcgattgtt tcaggacgtc gtccacttcc gcgagggctt
    13921 cgtcgtaccg gtggtcgaac gcaccgatgc gcgccttcgc gatccgcgcc gtggggaagc
    13981 ccggtaccag acggagtgcc gagtcgtaga gctggatcgc atcgtcacgc ttgttcttgg
    14041 tgatcagggc ctcgccctgg gccaccatca gcgcagcctt ggcctggggg tcggagagcg
    14101 actggttcgc cagctcggcg accttgtccc cctgcccgga cagcaccagc gccttggcca
    14161 gcagcggcac cgtctcgtca agcgagaagc cggcgtccat ggcgcgccgc agttcctttt
    14221 cagccccggc gtaatcgccg ctgtcgaaat tgatcttgcc gagcaggaag cgcgcttccc
    14281 cgaatcccgg attcttctgc aaggcgttct tgagctggat ggacgccgca ttgcggtcat
    14341 tcttggccag atactccttg gccgacgcca ccatcgattc cggactgcca ccacaggccg
    14401 ccagcaacag cgcacacagc acgctcaccg tggcgctgcg caccacgttc cgtccgcttc
    14461 ctttttgcat ccgcctgatt ccttgcatca cgtcgaggtc tctttcttca gtccgaaccg
    14521 gttcatcaag tcgtacaggg tcggacggct gatgcccagc acctcggccg cccgtgccac
    14581 gtttccattc acccgagcca tcacgcggat caccgcccgc cgctcggcct cgtcccgcac
    14641 tacacggaga ttgagggctt cgagttcacc ttcttcgacg gtgccatcgc ccacgccgat
    14701 atcgtccgcc gagatgcagt tgccgtcggc catgatgacc gaccgcttga tggcgttttc
    14761 cagctcacgg atgttgccgg gccagcggtg ggattcgatt gcctgcaccg cgtcgtcggt
    14821 cagcgtcatg gtgccgcgac cgttgctcgc cgcaaagcgc tggacgaagg cgtgggccag
    14881 cagcaccgcg tcaccatccc gatcccgcag cgccggaata tccacgacga tctccgcgag
    14941 gcggtaatac aggtcttccc ggaagcggcc ggtctggatc agtcccttca cgtcctggtg
    15001 agtggcgcag atgatccgca cgtccaccgg gatctcctca cgcccgccaa tccgctcgat
    15061 cacccgttcc tgcaggaagc gcagcagttt ggcctgcagc ggcatgggca gatcaccgat
    15121 ttcgtccagg aacagggtgc ccttgtttgc cacctcgatc ttgccgagtg tctgcttgac
    15181 cgcccctgtg aacgcgccct tctcgtaacc gaagagctca ctctcgagga ggttttcagg
    15241 aatcgcggca cagttgatgg ccacgaagcg ctcgttacgg cgcggcgaca gttcatgaag
    15301 cccgcgggcc agcacttcct tgccggtacc actctcaccc agcaacatga ccgtcacgtt
    15361 ggcggaggca accttttcca ccagccggca cacccgcagc atgccgggat cgcgcgtgat
    15421 gacgccggac agcgcgctgc cctgctgcat cttgaggcgc cggttgtcct gctggaggtc
    15481 gtacagacgg aaggcccgct ccagcgtgag gaccagcatt tcgggttcga agggcttggc
    15541 gaagaagtca taggccccca ttcccaccgc acggaccgca ttttcccggt cgtgctgccc
    15601 ggtgagcacg atcaccttgg tgtcgggcat cagcgcgagg atctcgccaa gcaggcggaa
    15661 cccctcggtc acgtcatccg gggcaggagg cagccccagg tccatggtca ccacggccgg
    15721 ctcgtagcgc cggatctgcg cgatcgcctc gtcccggtta ctggccacca ccgtatcgta
    15781 ctggtcgaag gcccaccgca tctgcttctg cagcgcgggg tcgtcttcga caatgagtaa
    15841 ggtcctgcgt ttatcgctca tgtctgctta gccgccggtt cgccatccac gccacccggg
    15901 agatgcgcaa gaggcagacg gatctcgaaa catgatccgg caccgacctc gctctcaacg
    15961 gagagactgc cgccgagctc gtggatatat tgccgggctt cgtatgcacc gatgcccatg
    16021 ccgctggcct tggtggtctg aaagggcttg aataactggt cccggatgaa cgctgcggac
    16081 attccggaac ctgtatcggc cacccggatg ctggcgaaat taccactccg ggatacctcg
    16141 accgacacac taccatgctc cgatgtcgcg tccagtgcat tttgcacgat atgccccacg
    16201 atacgttcca agcgttcgga atgcgccagc acggccagat caggcccgcc cgacaccagg
    16261 acgggcgcag gacggaagag tcgtttggat tgaacgatat gctccaacaa tggtgtgagc
    16321 gggacgggct tgcgctggtt gatgggcgtc ttctccatca actgggccat caggccgcgc
    16381 atgcgctctt ccacgtgtcg caccgtgtcg agcatgtcgg cctggaactc gggattgtcc
    16441 ttgtgacgct cggcattgcg caacatcaga gagagctgtg caacgaggtt tttcaggtca
    16501 tggacgacaa aggccgacat ccggttgaat gcatcgaact tgcgtgcctc cagcagcgct
    16561 tccgcggcct gcattcgcgc aaaatagccg gcggcctgac gccctgccgt cttgagcaga
    16621 tcgaggactt cccagttcac gtccaccggc gtgcgcggcg tcaacaggat cacgaagccg
    16681 atcaggctgc cggccgagat gaggggcacg atcagccacg catcgtccac ttcgccgatc
    16741 caggcgggca cgggctggga ttcgccgaaa ttcacccctg cgcgggcttc ctccagattg
    16801 accacccatt ccttttcgag caagaagcgg atgaatgcat cgtcatcgcc gacctgcatc
    16861 ccgcaatggg gcatgttgag ccgtgcctgc tgactgtagc gccccgctgc atccttgagc
    16921 cagaccccgc ctcccggact ttccaccaga tcgcccagcg cgttcaccac ggattcaccc
    16981 agagtgagcc cgtctccggc cgccgacagc gcctgcgtga cgcccagcca ctcggtccgg
    17041 tagtcgtaac gataagcaaa gaaatgtttg gcaatcagca ccttgaagcg tgatcggacg
    17101 gccccggaga acgccacgac gcccaggaac agggcgccgc cgaacagcag ggccacctgc
    17161 agggcctgtc cccactcgcc accgaagtag cgcatgtaat agccggcgcc cgcaatcaag
    17221 gtcaggtaaa gccccgtccc ggccatggcc gtcgaatgca gcacgacgtg ccgcgagacc
    17281 gagatgcgga aggtccattc cgggttgcgc gacgccgata cggcgatgag cgggattgcg
    17341 agggtgtggg caaatccgcg cacgctccag accgagggat gcaggcgcgt gaacagcagc
    17401 gcgtccgaga agaggtagac atcgaacgca aacaggccgc ccagcgcgag ggtcaaaggc
    17461 ttgatgcccc aatgggagcg ctccggcacg gaacgataga agtgttccag aagcaccaga
    17521 ccgaacaccg acgaagccag cgatgtggcc gcccaggtgc gcatcggcgt cccgtaaagc
    17581 gggatgccga gcgtgaagca cgccacgccc cctgccgcca gggccacgat cgccagcatc
    17641 gcgggccgcg gccaggcgag tgcagcgacg ttcccgtccg cctcgctggc gttctgcaac
    17701 agcatcgcca ccaggaagcc gaaccacgcc gcctgacgca ggacatcgaa cagattgccc
    17761 agctggaaca gccagatcca gcccgtcagc gcaaagcccc agctcgcagc cgcccaacca
    17821 ccgcttaata cgacagcggc gaccagcagc cagccgcggc gcccccgccg ccaggcaatc
    17881 cccagataca gcgcaaacaa cccatgcagg accgtcgcga aggcatagct ccacacggac
    17941 acttgggaca ggaagagttc cattcaggct ggacaggaaa cgggcgaccc gatgaggggt
    18001 cgcccggaca ggcaggttac gcgcacgacg ctagcgcagg tcagtgggca ccgtcgccgg
    18061 tcaggaccac acggacagtt tcgaagagca cgacgatgtc gaggaacagc gtgtggttct
    18121 tgacgtagta aagatcgtac tggagcttgt tgatcgcgtc atccaccgac gagccgtact
    18181 ggtaacgcac ctgagcccag cctgtgacac ccggcttgac gcagtggcga acggcgtaga
    18241 agggaatgtc cttggtgagc tgatccacga agaagggacg ttccgggcga gggccgacga
    18301 gactcatgtc acccttcagc acgttgaaga gctgcggcag ttcgtcgatg cgcgtcttgc
    18361 gcatgatccg gccaaaacgc gtcacacggt catcgttggc cgacgcccag cggggcttgc
    18421 catcccgctc cgcatcgcgg cgcatgctgc ggaacttggt gaccttgaac aggcggccat
    18481 tgagaccgac ccgctcctgg cgatagaaca ccggcgcgcc gtcctccatc acgatcagca
    18541 gcatcgccag cagcatgacc ggcgtggcga gggcgagcag gatcagggag gccacgatgt
    18601 cgaacacacg cttgaccagg gtccggccca gcccctgacg gaaaccttcg ccgtagatca
    18661 gccagctcgc ccgcagggaa tcgatgcgga cctgaccgcg gacccgttcg aagaaactgg
    18721 agaggtcaag taccttgatg ccggccatct tgcattcgag cagttcccgc agcggcagca
    18781 cgccaccgcg acgctccttg acggcgacga tgatctcatt gacgcgcaga tcgttcgcgg
    18841 cagccagcag gcgtccgtcg taatggacga tgcgcctcgg gtcgacccgg acctcatcgc
    18901 caccactgcc cgccgggaag aacccgacca cctgcagact cggattggcc ggatgggtga
    18961 gcgcgtgctc caccgcgacc gcatcggcac cggttccgat caccatcaca cgcttcgcca
    19021 acacacccac tcccatcttc cgcatcgtaa aaccgcgtac ggccacgaca aagccaagca
    19081 atgcgatcac gtcgagctcc atggcctcgt gcgcgaattc gccccacggc aggacccgga
    19141 agattccgta cgccaccggc acgctgagca cgatggagat caacaccctc accgtggttt
    19201 cccgcaaatc tgcgccactg cccgcccgat acagcccgag ggcaccgttg agcaccatca
    19261 tggtgatggc gaacaccagg gccgaaggca cgaccacgcg gaccagactc tcgtcgagct
    19321 gggtctgcag gaggaacacg accacgacgg agacaaacag caacgtcacg tcgaacatca
    19381 gctgcaggat cgtattcgac ggaacgtagt gattgaaaag tttgagcatt ccgaatttca
    19441 gcccgcttct cagcaggccg cccatgggat gagggttcga gcactgatgg tcggggcccg
    19501 cgacaaccgg caaaaattgc cggcaattat cgccgatctg ggcacccgat caccctccag
    19561 tcatgccgat tgtccgtcac gaacttgcca attgcgtgac tacgaccacg gacgaacaat
    19621 atcgcctccc gcgtcgcagc gctgtgaagc cgttcacaca aaaatgacag agcctttccg
    19681 gcaagaccaa accgctacga cacaaacgga gcgattctaa tgccctgggc atagaagaaa
    19741 cgttaatctc tatgtcttgc aggcgtttag gggcacaacc accctgcctc ggcgcaacaa
    19801 agtgtgtccg cccctgcccg attcaggcgt gttcgcgtcg ggaaccgcct acaactcgat
    19861 ctgggtgccg aactcgacga cacggttgac cggaatgcgg aagaagtccg ccgcgctgtc
    19921 ggcgttgcgg aacatgatgt taaaaagatg ctcgcgccac aggggcatcg ccgatccctt
    19981 gcgcgggatc agcgtttcgc gccccaggaa gaaggacgac tgcatcagtt cgtagggcag
    20041 gcccttttcg gcacacagcg ccagcgcggc gggaatatcc ggatcgtcct tgaaaccata
    20101 gcggacgaag acccggaaga acccgaaatc cagttcctcg aggcggacgc gctcttccag
    20161 gggaacgtag ggcacatcgg ctgtcaccac atggaccagc accacgctgg cgtgcagcac
    20221 cttgttgtgc agcatgttgt ggagcaacgc ccgcggcacc ccgtccggat tggtggtcat
    20281 gaagatcccc agcccgggca cccggtgtgg cggatcgtga aacatggact ggatgaacag
    20341 atcgagcggc atcgtatcca cccgcatccg ctccgtgagc agcgcccgac cagtcttcca
    20401 ggtcatcagc agcgtgtgaa cgcccagccc gagggccagc ggaaaccacc cgccgtcgag
    20461 gatcttcaca ctgttggcgg cgaagaacga gaaatccacg gagaagaaca aggcgacgaa
    20521 ggcaaccgct gcactccagc gccagtgcca cagggcacgc accacgacaa acgcaaggac
    20581 ggtgtcgatc atcatcgtca gggtcaccgc aatgccgtaa gcggaagcca gcgcgctcga
    20641 cgacttgaag ccgatcacca gcgcgatcac ggcccccagg agcatccagt ttacgttcgg
    20701 cacatagatc tggcccgctt cccggtccga ggtatggacg atgtggatgc gcggcgagta
    20761 gcccagctgg acggcctgtt tggtcaacga aaacgccccg gagatcaccg cctgggaggc
    20821 gatgatggtc gccaccgtcg agagcccgac catggggtag agcagcggat ccggcaccag
    20881 caggtagaac ggattggcga ccgccgacgg atcgtcgatg atgagggcgc cctgaccgag
    20941 atagttcaga tagaggcacg gaaagacaaa gccgaaccat gccagcttga tcggccgccg
    21001 accgaaatgc cccatgtccg cgtagagcgc ctcgccgccc gtgatggcga gcaccaccgc
    21061 gccgagggcc agaaagccat gaacacggtt ctccacaaag aaccggacgg cgtgcagggg
    21121 gttcagcgct cccagcacgt ccggatggcg cacgacgttg aaggcgccaa ggactccgag
    21181 ggttgcaaac cagaacagca tgaccggccc gaatacggca ccgacctgcg ccgtaccctt
    21241 gcgctggatg acgaacagcc cgatcagcac gaccagggag atcggaatca cgaagggcct
    21301 gaatgcgggg gtcgccacct ccaggccctc caccgcggac aggacggaaa tggccggggt
    21361 gatcacgcca tcaccgtaga acagggccgc accgaagagt ccgagtgccg ccatcaggga
    21421 tgccgcacgc ggcgagtcgg tgctgcgcaa ggccagcgaa gtcagcgcca tgatgccgcc
    21481 ctcgccccgg ttgtcggcac gcatgatgta caccacgtac ttgagcgaca ccacgaaggt
    21541 cagagcccag aacaccaggg acaggatgcc cagcacgttt tccggtgtgg cgggcacggc
    21601 atgcgggccg ttgaacactt ccttcatcgt gtacagcgga ctggtgccga tatccccgaa
    21661 caccacgccc atcgctgcca gcgccaccgg caataccttc gtacccgaat gcccgtgctg
    21721 atcttccgcg acaaccgccg acattccact ctccccgact catagtcact gccgcccgga
    21781 ccacggccgg cggcgggcgc attataggtg aagtatgctg cgctgccgca gatcgctgaa
    21841 cttttcgagc ggggcccggt cacccgtcct tcatgccgag ccgttccagg cgatagcgca
    21901 gggacctgaa ggtcacgcca agcgcccggg cggcggccgt gcgattgccg cccgcgcgct
    21961 ccagcgcctc cacgatggcc tgacgctcta cgtgatccag gtagtcctgc aaaggcgcgc
    22021 caccgtcctg caccacgatc gacggcgatt cgcccgccag ctcctcggca ggctccagca
    22081 acagatcggc cgccgtgatc acgttgccat tggacagcgc cagcgcccgc tccagaatgt
    22141 tttcgagttc ccgcacattg ccgggaaagg cgtacccgac gagggcctgc agggcatccg
    22201 ggccaaggcg cgggacatcc atcccggaat tttcggcgag acgccccagc agggtggtcg
    22261 ccagctccgg gatgtcctca ctgcgttccc gcagcgccgg catgcgcagt tcgatgacgt
    22321 tgaggcgata gtagagatcc tgccggaagc tggaggcttc gaccatcgat ttgagattgc
    22381 gatgggtggc gctgatgagg cgcacatcca cgccgacctc ggtggtgtca cccaggcgcc
    22441 tgaagcgctt ctcctggatc gcccgaagca gcttgacctg catgggcaac ggcaggtcgc
    22501 ccacttcgtc aaggaacagc gtgcccccgt gagccgcctg gaagaagccc tcccgatccg
    22561 catcggcgcc cgtgaaggcc cctcgcttca cgccgaagaa ttcgctttcc atgaggctct
    22621 cggggatcgc cccgcagttg accgccacaa agggcgcacc gctgcgcgga ccaagctcgt
    22681 ggatcatgcg cgcggcacgc tccttgccgc tgcccgattc gccggaaatc agcaccggcg
    22741 cctgacttcg cgccagcttc tcgatcagcg cacgcacctg caccatggcc gctgaatgac
    22801 ctcccagact ggacgtatcc cgggtgccct ccacgccatc cgcgggcgac acggagaaca
    22861 cggacttcac cagggcccgc agctgatcca ccgacacagg tttgctgagg tagtcgaagg
    22921 ccccggcctt cagcgcggtc acggcattgt ccatgctgcc gaaggcggtg atcaccgcca
    22981 ccggcaggtc gccgaccttg tccgcaatga aacgcaccag atccagccca tccccgtcgg
    23041 gcagacgcat atcggtcagg cacagacgat agcgccgttc agtgagcagg cgctgcgcgt
    23101 cggccaccgt gcccgcaccg tcgcaggcca ggcccatgcg catcagggtc agttcgagaa
    23161 gttcgcggat gtcgtcctcg tcgtccacga tgaggacgtc cggcccgctg gcgcgggcac
    23221 gtcggtcgat ggagttcatg atctgctgcg tgccgtgagt cgaaaatggg caccgggcag
    23281 gccgcccgcc tcggcctggc ccagttcgag cacagcaccg ttggcatcgg ccagttcccg
    23341 ggcaatatag agcccgagac cggttccctt ggaatgtgtg gtgaagaagg gttcaaagac
    23401 ctggttgcga agcgcctcag caatccccgg gccgtcgtcg gtcacgtcca ccgcgatccg
    23461 gccgtcgtcc cgcagacccg cggcgatgcg gatggagccc ggcgcaccgc tgccatgccg
    23521 ctgagcgttg gcgagcaggt tccacaggat ctgacgcagg tgcgtacgat ccatcagcag
    23581 ggtcaggtcg gatgcaatcg ccacatcgac cagcgcgcgc cgcaccggat cggtgagggc
    23641 gaattcgtcg agaaattcat ccacgaactg tttcaggggc agcacttccg gcagggcctc
    23701 gtcgcgccgc cccagcgcca gcacatcgcc gaccagccgg tcgatgcgct gcgcgttggt
    23761 gccgatgatg cggatcaggc gggcctgcat gtcgccccgc ttttcgtcgg acagcagctc
    23821 cgcagcgtgg gagaccgccg ccagcggatt gcggatctcg tgggcgatac ttgccgtcag
    23881 ccttccgagg gcggccagct tgacctgctg gatctggtca tggatgaggt cgaaatcctc
    23941 gaggtagacc agggtcgcgt ccgaatcacc ggccctcagc atgcgcgcca gcaggagctt
    24001 gccgctccct tccgcgcgaa aacgcagggc cgaatgggca cccggctcgc cgtcctcacc
    24061 cgcccccagc ggtccggcca gtgctggcgc acagctggcc agcgcaccgc cctcgcgcag
    24121 cgttccgccc agcagggcat tggcctgagg attgaagtga cgcaccacgc cgccgggctc
    24181 caccaccagc acaccgtcct gcatgtcctg gatgatctgc tggttgaggc gcaactgcgc
    24241 ggccagatcc gcaccccgtt gacgcgccag ggtctcgttg gcgatcgctc gcaaggccag
    24301 caagcgcgcc acctgggcaa tcgtgaagaa accgatgcag gtcacgccga cggtgaagaa
    24361 atccaccaca tcgcgcccgg agaggaagcg cagcagattt tcgaacagca ccgccactgt
    24421 ggcatacgct gcgtagaaca gcaccatccg accctgcccg accagcccag aaccggccag
    24481 gacgatcatg atcaggatgg gcatgccgct gcggtagcca ccgctggccc acatgatcac
    24541 cgtgagcagg gtgacgtcca ccagcacctg caccgtcacc agacgctcca tgcccaggtg
    24601 acgggccgca tcggggaatc cgaggatcag aaccgccgtg aggtaggcaa acgcggcgcc
    24661 gaagaacagg gttgccgaat cgccccccag gctgaggccg ggcccggcca ccagaaaaac
    24721 gccggccagc accaggcgga agagattgag atagcgcagg ggctcccacc gcccacggaa
    24781 ctccgtggcc ccggattcca gccccgggta cgccactgtc ttcacacccg cggaccaagc
    24841 cggcggtggg cgtccgagca gaagaaatac ccgccgacgt gcagaccttc gctttccggc
    24901 acatgcaccc cgcaatgggc gcaggccacc atcgcctcgg cctccgtcgg ctgccggggc
    24961 gcttcggagc gcgcctcccg ccccgccccg ggtgtgtcgc cggactttcc ttgcaaggca
    25021 cgccgcacgt aatacaggcc gatcaggaac aacacgaaga caagcagatt tctcaccgga
    25081 acgcctccag actgacgggc cagtttagca gaagcgtgcc agccgctcgc aggcctccgc
    25141 cagggtcgcc tcctgcttgg cgaagcagaa gcggatcacc ccttcatccc gcccgtcacc
    25201 gaaaaaggcg gacaggggaa tcgccgccac gcccacctcg cgggtgagcc actcgacgaa
    25261 cgccaggtcc ggcaggtcgg acacggccgc gtaacggacc gtctggaaat aggtcccggc
    25321 gcagggcagc aattcgaagt gtgtcgccgc cagccccgcc cggaaggcgt cccgcttggc
    25381 ctggtagaac gcagccagcg cgagatggcg ggacggctct gccatgtaat ccgccagggc
    25441 cagctggacc ggggcattca ccgtgaacac attgaactga tgcaccttgc ggaactcgtc
    25501 catcagggcc tgcggcgcga gcacgtagcc gaccttccag ccggtcacgt ggtaggtctt
    25561 gccgaagctc gagacgatca cgctgcgcgc cgccagctcc ggatcccgcg cggcgctttc
    25621 gtggcgggca ccgtcgaaga cgatgtgctc atacacttcg tctgccacca ccacgatgcc
    25681 cgtgccgcgg gtcaccgccg ccagctctat catgtccgcg gccgaccaca ccgcgcccgt
    25741 cggattgtgg ggcgtgttga tcatgatcat ccgcgtccgc ggcgtgatca gcgcccgcag
    25801 cgcggcccag tccggccgat attccggcgc gccaaggcgg acacgacgga ccactccgcc
    25861 ctgcagttcg atggccggga cgtaagagtc atagaccggc tcgaagacga tcacctcatc
    25921 gccgggatgc accagcgccg ccaccgcggt gaacaaggcc tgagtggcgc ccgccgtgac
    25981 cgtgatctcc gctcccggat cgtagtgcgc accatagagc gcatgcacct tggtggcgat
    26041 ggcgtcgcgc agcgcgggca tgcccgccat cggcgcgtac tggttgaacc ccgcccgcat
    26101 ccagtgggcc acgcgttcga agagcacgtc ctcggcgctg aaatcgggaa agccctggga
    26161 caggttgatc gctccgcagg tctgcgccag ctggctcatg cgggtgaaga tggtggtgcc
    26221 gacggtcggc aatcgcgaga ccagcggcgc ggaaaagacc ggcatcagtg acaaggcccg
    26281 aaaaggtgtc ggtccatttt aacgccatgc cgcgtgtccg tccgaggtgc ccggcggcta
    26341 caattcctgc atgcagaccc catcgaacgc ccccgtgacc gacacctgcc ccaccctgcg
    26401 ccgggagggt gacagcgtcc tgatcctcga ccagacccgc ctgcctcacg aggaggcctt
    26461 cgtccgcctc gccacactgg cggatgcagc cttggcgatc cgcagcatgc aggtccgcgg
    26521 agcaccgctg atcggcgcca cggccgccta tggcgtcgcc ctggcgctgg catccgacgc
    26581 ctccgacgca gccctcgccg atgccgacgc ctgcctgcgc accacccgcc ccacggccgt
    26641 caacctgcac tgggccctcg accgcatggg ccggaagctg gcgtccgtgg cgccggccgg
    26701 ccgccgcgag gccgcctggg gcgaggccga cgccatccgg cgcgaggacg ccgacgccaa
    26761 cgcggccatc ggacaacacg gccttgccct gctgaacgcc ctggccgccg agccaggacg
    26821 accggtccac gtcctgaccc actgcaacgc cggctggctc gccacctgcg gccatggcac
    26881 ggccctcgca ccggtctatg cggcccgcgc cgcgggcctg ccggtccatg tgtgggtcag
    26941 cgagacccgt ccccgcaatc agggcctgct cactgcctgg gagctgcgcc gcgacggcat
    27001 tccccacacc ctcatcgccg acaacgcggc cgcctggctg atggccgaag gacgcgtcga
    27061 ttgcgtcatc gtcggcgccg accgcatcgc cgccaatggc gacaccgcca acaaggtggg
    27121 gacctgcctc aaggccctcg ccgcccgcgc ccacgctctg cccttctatg tggcggcccc
    27181 tctgagcacc gtggaccgca gttgccccaa cggccgtgcc atccccatcg aagcacggga
    27241 cggggacgag atccggcgga tcggtggact ggacgacaac ggacggcccg ccttcgtgca
    27301 catcgcccca ccggacagcc cggtctgcaa cccggccttc gacgtcaccc cggcggccct
    27361 gatcgacggc atcgtcaccc cggtcggcgt gtgtgccgcc cacgacatcg gccggctcgc
    27421 cggcagcctg agtggagact ccccatgatc gagaatgtcc tgcgcgaagc cctcctcgcc
    27481 accgcccagt ccatgggcag cctcggcctc aacaccggca cggccggcaa tgccagtgtc
    27541 cgctgtcacg acaacctgct catcacgcct accggctgcg attacgccag ctgcacgccg
    27601 tcggacatgg tgctgatggc catggacgga acctggcagg gccactgtgc gccgtccagc
    27661 gagtggcgca tccatcggga catcctcgcc gcccgcccgg aagccggcgc cgtgctccat
    27721 gcccattccc ccttcgccac cgccctcgcc tgccaccgcc gcggcattcc cgccttccat
    27781 tacatgatcg cccgcttcgg cgggaccgac atccgctgtg cgccctacgc caccttcggc
    27841 acccaggccc tgtcggatgg tgcgctcgcc gcgctggagg gtcgcagcgc ctgtctcctc
    27901 gccaaccacg gcatgctcgt catcggcagc gacctgcgcc gcgccctggc cctcgcagtc
    27961 gaattcgaat ccctctgcgc ccagttctgg cgcgcatgcc agatcggcac gccgaccctg
    28021 ctgtcggacg ccgaaatggc cgacgtcctc gccaaattcc gccactacgg acagcccgcc
    28081 caggcggccc tgctcgccga cgcgctgccc gccgcggggc ccgccgccag cggactctcc
    28141 gacggctgaa cgttcgcgcc catgcatccc cacagccgca gcgtcctgct cggcatcgcc
    28201 ggcgcaagct ttattgccgc ggtgctggcc tggcacctcc tcggcgacca ccccgcattc
    28261 ttcctgccgg tactggccgt cctgatggcg gccccggtcc tcgcctgcct ggtgttcctc
    28321 ggcagaccgg ccgacaccgc gcggcaggcg tggcgcctgc tggagtcaag tccgatcgcc
    28381 gtggtcatca atcgccccga cggcaccccg ctgttcgcca acacgcgcgc ccacgatctg
    28441 gccggaaccg acgccgccgg cttcatggcc cgcccggtga tgagctggtt cggcgatccg
    28501 gattttcccc aggccgccct cgagcggctg cgcgccggcg aacgccttca cgacatggag
    28561 gtggagttcc agaccgtccg cggcgattct ttctggaccc tgacctccat ggaatccctg
    28621 gacctggacg gcaggccggc catcatcagc tggagctacg acatcaccgc ccgcaagacc
    28681 gccgagcagg aactgcgcaa gctgtccctc gccgttgagc agagtccgtc gatgctgctc
    28741 gtcaccgatc cggatggccg catccagtac gccaatcctt gctactgccg gaccgccggc
    28801 ctgactgcgg ccgaactggt tggccagccc ccggacctcg tcgatctgac ggacgcgccc
    28861 ctgcgtttct ccgccccccg tcaccccgaa ctcaaggacg gcggcgtatg gctgcaggaa
    28921 tgccgcctgc gcacggcaca cccacgcacg caatgggtgc gcgtcatggt gagtcccctc
    28981 atcgaggacg ccacgatccg ccattgcatc tgggtcctgg aagacctcag cctgcatcgg
    29041 gacgccatgg aggccctcga tcaggccaag cgcctcgcgg aggaggccgc cgcgtcgcgg
    29101 gcacgcttcc tcgccgacat gagccacgag atccgcaccc ccgccaacgc catcgtcgga
    29161 ctcacccacc tggccctgga cagcgggttg ccgccggccc aactggatca cctcacccgc
    29221 atccagtccg ccgcagacac cctgctggcg gtcatcaatg acgtgctcga cctctccaag
    29281 atcgacgccg gccgcctctc gctggagcag gcgcccttca gtctcgacga gatgatcgac
    29341 cgggtcatca ccctggtcgc acctgcggcc cacgcccggc acctcgccct gctgctggcc
    29401 atcgcgccgg acgtcccgct ccgccccgtg ggcgacgcac tgcgtctcgg gcaggtgctc
    29461 accaacctcc tcgcaaatgc cgtcaagttc accgaaagag gcttcgtccg gctgaatgtc
    29521 gtggtggcag cgcgcgatga aacgtccgca agcctgcact tcgaggtgga ggacaccgga
    29581 atcgggctgc cgcccggtga agtcgagcac ctcttcgagg ccttctccca ggccgacgat
    29641 tcggtgaccc ggcggttcgg cggcacgggt ctcggactcg ccatctcgaa tcacctggtg
    29701 acgctcatgg ggggacgcat cgaggtccgc agcgcccccg gtgacggcag ccgcttcggg
    29761 ttcacagtgc gtctgggcgt cgcccccgcc ccggcggcgg tcgaattgcc gagcgcgctg
    29821 cggggcgcac acaccctggt catcgacccc cacacagcgg cccgcgatct gctggtggcc
    29881 cagttgaatg cgctcggcgt gcccaccgag ggctgcgaca ccccggacgc cgccctcgcg
    29941 tgggcccacg atcccgaccg cagcatccgc ctagtcctgg ccgcggcccc gcggggcgag
    30001 agcggccctt ccctgaccgc cgccctgcac acccttgcag gccacggtcc gctgcccgca
    30061 ctggtgatca tggggccgtt tcctgcgcat cgcgaccacg ccggcctgcc agccggccac
    30121 ctgcgaattc ccgcaacccg cagccgcctg cgcgacgccg tcgtcgaggc cctcgcgccg
    30181 accagaccag agccccatga gctacccgcc cccccgccgc gcgccctcgc cggacgctcc
    30241 atcctggtgg cagaggacgt ggagatcaat cgcacgatca cccagaactt cctcgagaac
    30301 gcgggcgccc gcgtcaccct cgccaatgac ggcgacgagg ccctgcggct gctccagacc
    30361 atgggcgccc aggccttcga cgccgtcctc atggacatcc agatgcccgt tctggacgga
    30421 ctgagcgcca cgcggcaact gcgcgccgac agccgcttcg cccacctccc ggtgatcgcc
    30481 atgacggccc atgccatggc ggacgaccgg gaccgctgcc tgcaggccgg catgaccgac
    30541 cacgtggcca aacccatcgc gccagaacgc ctcatcgacg ttctgatccg gaacaccatg
    30601 cgcgcccccc agccggctcc ggccgcgggg ccgcaacccg ccgccgtctc acacccctcg
    30661 gacctgccac acatcgccgg catcgacgtg cctggcgccc tgccgcgctt caaccacaac
    30721 gtcgatatgt acctgcgcat cctgcgccgc ttcgcgcccc accacggcga cgcccccgcc
    30781 cgcatcgaag ccgccctcgc cgccggccag cgtgacgacg ccgagcgcat cgcccacacc
    30841 ctgcgcagca gtgcggccgg tctgggcgca gacggcatcg ccgatctggc ggcacagatc
    30901 gaactcgggc tgcgcaaggg cgccggcatc gacacaccgc tgcacggcct gcatgaggag
    30961 atgacccgcc tcctggccgc tctggaccgg gcactgccgc cggaagaagc cccgcggccc
    31021 ggtgcgcccg aactcgacgc cgaatccctg gacgccgcgt gtcgcgagct cgacgccctc
    31081 ctgcggcatg ccgacggacg cgcgcccgcg ctgttcctgc gcatccgtaa cgatctttca
    31141 cgtcgccacg cgcctgccgc cgtggaaact cttgcagacg ccatcgcccg ttacgattat
    31201 gataaagcgt tgatacacct cgctaatgtc atgaaccgcg ccccggcgcc gtgaaccgcg
    31261 ccgcgccccg gagatcacac ttccccgtgg aacagaccga ccccacatcc gaccaggacg
    31321 atcaccgcgc catcatcctg gtagtggacg acgcgcccgc cgccatcgag cccattgtcg
    31381 actgcctgca tcgggccgac ttccggaccc gcatcgccac cacgggcgag cggggcctcg
    31441 ccctcgcgcg ggtcgagccc ctccccgcgc tgatcctcct cgacgtcatc atgcccgggc
    31501 tcgacggcta tgaggtctgc cgtcgcctga tggccgatcc cgtcacggcc aacatcccgg
    31561 tcatcttcct gaccgcgcgc aacgacgtgg tggacgagca ggccggattc gacgccggtg
    31621 cagtggacta catcaccaag ccgatcagcc cgcccacggt cctcagccgc gtccgcaacc
    31681 acctcgtact caaatcggcc agcgattttc tgcgcgaccg caacacctat ctggagcagg
    31741 aagtcctgcg ccgcacgcgt gaactgagcc tcatccagga cgccaccatc gtcgccatgg
    31801 cctccctcgc cgagacccgc gacaacgaga ccggtaatca catccgccgc acccagatgt
    31861 acctgcgcgc cctggcgcgg cacctgcagc accacccccg cttcgccgcc gagctctccg
    31921 acgagaacat cgacctgatg tacaagtccg cccccctgca cgacatcggc aaggtgggca
    31981 ttcccgaccg catcctgctc aaacccggcc gcctcacggc ggacgagttc gagatcatga
    32041 agacccacac caccctgggt cgggatgcga tcagcgccgc cgaatccctc ttcggcgtct
    32101 cggcgcgctt tctgcgctac gccaaggaaa tcgcctactc ccaccaggaa aaatgggacg
    32161 gcagcggcta tccgcaggga ctctccggtg accggattcc cctttccgcg cgcctcatgg
    32221 cggtagccga cgtctacgac gcgctcatct gccggcgtgt gtacaaaccg cccatgagcc
    32281 acgccgacgc cgcggacatc atgcgtgccg gacgcgggac ccacttcgac cccgacatcc
    32341 tcgacgcctt cctcgccctg ctggacgatt tccacgccat cgccctgcgt tttgccgacc
    32401 tggagcagga tcacgcccgc gagatcgaac gcctccgcca ggtgttcggc gactgagctg
    32461 ccgcctgtcc gccgcggagc cgattcaagt ccaccgcccc agggtcgtta tctgaggata
    32521 acggaggcgc ccgcccgagg caccccattc cccaggagtc cggatgattt ccagcacgac
    32581 ctcccgtcag gtcgtcgata ccatacggcg ctacgggctg gcgctgctcg cgtttgccct
    32641 ggtgctgctc gtgagcttcg agcgttacca ctcgaccgtc cagcaagagc aggcgaccac
    32701 ccacacgcgc ttccagctca tggcccagga gcagatcgcc cgtctgcgcg agcggaccga
    32761 ggagctgttt tcccacgcgg acgtcctcca gcactatgcc ggtcaaggcg gatccctgga
    32821 cccccagcgt tttgcacgac tgctgatgcc catggttgca cacaatccgg agattgcgtc
    32881 ggccggcatc ctgcgccgcc atgccgacgg cgcacccgtg gaggtcctcc aggtcaccgg
    32941 cgccacgcgc ctcacacggg gcgccgactt cccaaacagc gaagcctacc ccgacgccga
    33001 cgaccgccgc atcctcctcg aggcactccg caatgcgccc cacacggcta ccgggacggc
    33061 aacgcttcca gtggccaccc gcgacatcgg cggcacaccg gcggatacct tcctgatctt
    33121 cgcaccggtc cgcgaagacc cggacgcccc cccgggcaac caacaggatg ccgcctttct
    33181 ggagatccgt ctgagccgcc tcattgacgc ggccttccag cccgccatca accgggacgt
    33241 caatcagagc atcggcgtgg aggtccttga tgctaccgat gaggcccgca ccctgctttc
    33301 gtcaccgggc tttcgcccgg cagagctcac ctacgccgac gccgtaccca tgggcaatcg
    33361 ccgctggctg gtgatcatcg tgcccgacgg tccccactac tcgttgagcc ccagtgcgga
    33421 cgccggcggg cagctcttcg ccggcttcag tctggcggcc ctgatcgcct tcatggtgta
    33481 cgtgctccag agccgcaccg aagccatccg gcaccgcgtg gccgaacaga cggttgccct
    33541 cgccgaggcc aatgatgccc tcggacaata cgtcaacgcc ctgggcgaaa ccaaccgcct
    33601 cctcgaaaac gaggtagaca agcatgcccg cgcggaagcc ctcttgcggg agacgacctc
    33661 cctgcaacgg gccatcctcg actgcgccga gtacgccatc atctacaccg acaccgccgg
    33721 catcatccgg gtcctcaatc ccgccgccga acgcctcttc ggctaccggg ccgaggacgt
    33781 catcgggcgc gacacaccgc tgatcttcta cacggcggaa gacatcgcag gtctgaccct
    33841 cgccggcaag ccctcaggct tcgcggccct cgccgtcgag cccctgaacg gcgccgccga
    33901 cacgccaacc ggcgccgccg acccccgcga agtcaccctg cgccggcgcg acggggagac
    33961 ctttcccgcc aacatcgtgg tctccacggt acgcgatgcc agccgcaacg tccgcggcta
    34021 cctgggcatc gtcgccgaca tcacccaacg gcgcgcggcc gaaagccgca tccgccatct
    34081 ggcccattac gacaacctca cctccctgcc caatcgcacc ctgctcaacc agcacctcac
    34141 ggaggtgatc gaacaggccg ccgtggatca gcgccgggta gccgtactgt ttaccgacct
    34201 ggaccgcttc aagtacgtga acgataccct cggtcatcag gccggcgacc agctcctgct
    34261 ctccgtcgcc cagcgcctgc gcggctgcgt gcgcgacggc gatctggtgg cgcgcaccgg
    34321 gggagacgag ttcgtcgtgg tgctgagccg gctggaacat gccgggggcg ccgaggaggc
    34381 cgccgggcgc atcctcacgg ccctcgcccg tcccttcgag ctgtgtggcc agcaattcac
    34441 cgtcacgccc agtatcggca tcgccctgta ccccaacgac ggcctggacg gcgaagccct
    34501 catcaagaac gcggacgccg ccatgtacct ggccaaggaa cgcgggcgca acaacttcca
    34561 gttcttcgac cacgggctgt cggaacgcta ttccgaacgc ctcgaactgg aggcacgcct
    34621 gcgccaggcg ctgcaggcac gggaattcga gctctactat cagccgcagg tggacaccct
    34681 gtcgggcgag atcatcggca tggaggccct gatccgctgg cctcagcccg acggcagcct
    34741 catcccgccg gacaagttca tccccatcgc cgaagagtgc ggcctcatcg tgcccatcgg
    34801 cgcctgggtg ctggaggaag cctgccgcca gaatcaggcg tggatccggg agggaatcgc
    34861 ccgcgtacca gtgggcgtca acctctcggc gcggcagttc gacgacaagg acctgctcga
    34921 caccatcaag tctgcactcg acaccgccgg gctcgccccc gcctacctgg atctggaact
    34981 caccgagagc ctggtcatgc gcaatccgga acacacccgc aacctgctgg tggaatgcaa
    35041 gcggctgggc ctgcaactgt cggtggatga cttcggtacc ggctattcca gtctggccaa
    35101 cctgaagcgc ttcccgatcg accgtctcaa gatcgaccgc agcttcatca aggacatcgc
    35161 gtccgaaccc gacgacgcca ccatcgccca gaccatcgtg gccatggccc acaccctgcg
    35221 cctggaggtg atcgccgaag gcgtcgaaac cgaagcccag ctcgccctgt tgcgccgctg
    35281 gcgctgcgat gcttaccagg gctacctgtg cagccgaccc atcaacgccg cggctatgac
    35341 gcagatgctg cgcaacctgc gcgcctcacg cctggtgtcc ctcaccgccc tctgaacacg
    35401 accgcgctca gcggggctgc agacgggtca gggacacctc gccactgcgg tcaaagtcga
    35461 tggaaccgaa acgcccggtc accagccggc ctttcaggcg gtagggcacc gcctcgggcg
    35521 ccttctggaa ctggcgcaat tggttgagga cgtccatcag cccggtggtg gccgtcacct
    35581 ccagcgtcgc ctcgcccagg cgcggaatca cggtcgcctg gttgctcacg ccacgggcaa
    35641 aggcatgacc gttcagttcc accgcgaaag tcagcccttc cacgtcgatc tcgacgttgt
    35701 tcgggttgag cacccgcagg gtgatcagga agcgctgctc cagcagcgtg gcatccacgg
    35761 gctgaatgcc ggccacgcgg acctctggcg cgtccagccc gaggcgcatc accgcgcacc
    35821 cgcccaaggc cgtcagcgcc gccgccgcga ccgtcaaaaa tcgccgtctg ccccgttcca
    35881 tctttacgct ccgtgtctgc ccgccggctc cagcatcttc agcaggctcg ccgtatcgtc
    35941 atgacctcca cccgctgcca tcagcgcatt gagctgctgc caggtctggg ccgcaaccgg
    36001 caagggtacg cccccttgcc gcgccgccgc catcaggatc gcaaaatcct tgtggtgcag
    36061 gcgcgactgg atgcctgccg caaagtcccg ccgcgccatg cgcccgccca tcacctccag
    36121 cacccgcgac gccgccgaac cgcccatcag cgccgtccac agccgctccg ggtcgacccc
    36181 gtgggcgttg gccaggcaca tcgcctccgc acaggcctgg atcgcgttga ccatcaccat
    36241 ctggttgcag gccttggcga cctgcccagc cccggcagga ccgatatgga ccaccgtacg
    36301 ccccagacag tccagcagcg gccgtacctg cgccagtacg gcggcctcgc cgcccgccat
    36361 gatggccagg gtcgcatccc gcgcgcccac ctcacccccg gacaccggcg catccagcca
    36421 gcccacgccg cgctcggcgt agcacccagc caactgccgc gcggtggcgg gcgcgatggt
    36481 actcatgtcc acgtgcacgc tgccgggggc aaagccggcc agcagcccgt ccgcccccag
    36541 ggccagcgcc tccacatccg cgcaggcggt gacgatggag atcaccacct cgctgcgcgc
    36601 cgccagggcg gccggtgatt cacaccaggc cgctccggcc gccctcaagg cctcggccga
    36661 ggccgggcgt cgcgcccaca cggccagatc gtgaccggcc gccagcagat gctgggccat
    36721 cggccgcccc atcaccccca gtccgacgaa tccgacccgc atgctcaggc tccggacagc
    36781 tgttccagca gcttgagggc cgcgatggag tcctcctcac ccagcccgct gcccaccatc
    36841 gcgttgaaga gctgggccgt cgccgcgctg ctgggcagca tcaggcccag gcggtgggct
    36901 tcctgcatca cgatgttcat gtccttctgg tgcatccagg ccttgaaacc gggcttgaag
    36961 ttgcggtcca gcatgcgctg gccgtggttc tccaggatgc ggctgtaggc aaagccgccc
    37021 agcagcgcct cccgcacgcg ggccgcgtcc acgccgctct tgcgggcgaa gttgaaggct
    37081 tccgccaccg ccatcacgcc cagccccgta aggatctggt tgcaggcctt ggccacctgc
    37141 ccggcccccg aggcgccgat cagcgtggcg gctttgccca tgcacacgaa gagcggcgcg
    37201 acggtgccga aggccgcctc gtcgccgccc accatgatgg tcagcgcacc gttgatggcg
    37261 cccacctcgc cgccggagac gggcgcgtcg agcatgctca cgccccgctc cgccagtcgc
    37321 gctgcaatcg actgggccgc cgccggggcg atggtgctca tgtccacata gatcaggccg
    37381 ggccgggcgc cgtccgccac accgcggggg cccagcgcca cctgctccac atcggcggcg
    37441 tccgccacca tgctgaacac cacatccacg tcacccgcca ccgccgccgc gtcggccgcg
    37501 ccggtggcgc cggcgtccag gagcggcgcc atggaatcgg cccgccgcgc ccacacggtg
    37561 acggcatgtc cgcccttgag gagattcagc gccatgggac gccccatgat gcccagaccg
    37621 atgaatccga ctttcatgtt cgacctttcc gattaaaacg tgacgcaccg ggccttcagg
    37681 ccgcggcgcc gaaatacagc ctgagtccgt cccgcacgcc ccgcagcacc gtggctgccc
    37741 gcgccggaga gcgggataac agcaattcgc ggaaaactcc ccacgacgag taagcaatcg
    37801 ttgcaataaa aaatacaagc ggattagagc tcaatttccg agcaatcaaa aaacggtttc
    37861 gttgaaggta atacagacca agcatgccat gaacaacatg ccgcccgact agcgatggtt
    37921 ccgtaaattc agccgctctc ttacgttgca aaacaaattt attgacgtaa aacactggtg
    37981 tgatttttga agccaaataa ccatagaaag tgtcatctaa tgcaataaaa aaagatggct
    38041 ctggatagcc aatagcatcg actatagcgt gtgaaatcag cataccttca aaacatccca
    38101 cagatacagt ttggaaaagc tgcccttgcg agaacaatgg atcacgaata gatcggctta
    38161 atacacaatt tgggtagaac gtgcgccccc acacgttcgg gctcccgtca gcctcccgcc
    38221 ggcggccatg gatgcagccg ctgcgctccg aaaagctgag cagatccgcc agtccgtcgg
    38281 gcagcggctc cacgtcgtcg tccatcagcc agaaccagtc gtagcccgcc tcgaaggcca
    38341 ccttcagacc gtgatggaag ccgccggcgc cgccggtatt ggtcgccatc ggcacgtatt
    38401 cgatgagggg atgggacaga aagccggcgc cctcgagggc ttcccgcgtg ccgtcggtgc
    38461 tggcgttgtc gatcaggaaa atgcggtcca cgggccggct ctgggccaac aggccggcca
    38521 ggcattccag caggagcgcc ttacggttga aagtcaccac cacggcggcg atgcgttggg
    38581 tcatggtcgg aagtccggtt tcagttcagg tcggggacaa ttcaggggcg tgccgcatcc
    38641 accggcgcgc ggtgcgcaaa gtgggcgcgc accgcccagc ccatcagggc cgtcaccccc
    38701 gcttcggtga tggtgaccgc gatggccgcg ccgggcgcgc cgtagaccgg cgccagcagc
    38761 gccagcaggc ccaggttgac gacgcccgaa accagcagga tgcgcgagaa cagcttctcc
    38821 cgcccgagcg gcagcagggt ctggatgccg aacacgtttg acaggccgat caggcagggc
    38881 aagggcgcca tccagcgcac cacctcgacc gccgcctcga aggcctcgcc atacagcagc
    38941 cggacgagga ccggcgcgag gacgaagagc cccaccgaca ccgcgaaggt gaacgcgccc
    39001 tgccacttga gcagacgccg cagcgtgcac aaggccgcgg cgcggtcatg atgcatgagg
    39061 tggctcaccc gcgggaagag cgcctgggac accggcgccg tggcggcctg ggcagccttc
    39121 agcagcttgt cggcggcggc gaaatggccc accgccgccg gccccgagag ccagcccagc
    39181 aacaccgtgt tggaggtggt gtacaggctg atggccgccg tcgagacgaa gacgtgccag
    39241 ccgtccgcca gtgcggcccg caggtcggcg agggccggcc gctgcatcgt cacggcccgc
    39301 gaacgggcca gttccgccag gcaccacagt cccgacagca ggcccggcgc ggccgtcagc
    39361 agcatggccg caggcagatc cgccggcccg cgcacgccgg cgacgatcag cggaatcgcc
    39421 agcgccttgg gcagcagggt cgccaacgcg gcaaaatcgg cccgctccac gccctgaaag
    39481 taccaggtgg gcgtcagcac gctgccaatc accgccagat agcccagcgc cagcaggccg
    39541 gagatttcac ccagcgttga gctcagggcc gccaggacga gcagcatcag cccgccgaca
    39601 aaggcgagca gcgccttcac gcccagcgtt gcccagaagg tccgcgagcg cgcggccttg
    39661 tcgtcccgtt gcacggcgat ctggcgggtg gcgctcaggt tgaagccgta gtccgccagc
    39721 agcacgaaat aggcgacgac cgccagcgcg aaattgaggc ggccgtaacc gtcgacgccc
    39781 agcgcccggg tgagccaggg ctgggtgagc aggggcaccg cgaagttcgc ggcctggatg
    39841 acgtacaggg acgcgagggt gcgccgcagg gaagccatcg gcgccggtca gccctgggcc
    39901 gcggcgatgc ggtcgaacac ctgcagggcg ttggccaccg cgtcatccat gtcgaagtaa
    39961 cggtactggg ccagacgccc caccgccacc acccgtgtat ggccgccgag gcggtcggcg
    40021 taggccgtgt aggccgcctt gctgcgctcg tcgaagatcg ggtagtaggg cacattcgcc
    40081 tccggatcgg cgggatcgta ggcctgggga tattcgtaca cgatgctggt gcccgcgcac
    40141 tgttgcccgg tgaggtgctt gaactcggtg atgcgggtgt agcggtgctc gttggggtaa
    40201 ttcaccgtgg tggcgggctg gaagaccggc accgcatggt gctggaagtc gaagcggatg
    40261 gagcgataag gcagagcgcc ccggtcgcgg ccgaagagtt cgtccaccat gccggtgaac
    40321 accagcaggc cgccaaaggg tgcgccctcg aactgcaccg cgccggtgtc cggatcgaag
    40381 gaccccaccg cgttgaacgg cgtatcgagg cgcaccgtga tgtggggatg ggccagcagc
    40441 ttctcgaaga gcttggtata gccctccgcc ggcacggcct ggtacggatc ggtgaagtag
    40501 cggtcgtccc gatccaccac caccggcacc cgcccggtca cttccggcga gatctcctcg
    40561 ggcttcacgc cccactgctt ggtggtgtaa ttgaggaagg ccttgcgata gacgaagtcg
    40621 gccagctccc gcagcagcgg ctcgtcctct tccttgaggc gcaggatggg cacccgagcg
    40681 cccaggccga agcgggacac cagcgcctca cgcatgcacg tcgcggtgtc ggcgtcgaag
    40741 agccggtcga tgctgttgag gttgaagggg atcggcacca gctgtccgtc aatgctggac
    40801 agcaccttgt gctcgtaggg ccgccattcg gtaaaggcgg acaggtaatc ccacacgcgg
    40861 ggattgtccg tgtggaacag gtgggggccg tagcgatgca ccagcacacc gtgggcgtcc
    40921 ggctcgtcgt aacagttgcc gccgatgtgg gggcgctgct ccagcacgag gaccttccag
    40981 cccagctgcg acgcgaagcg ctccgccagc acggcgccgg aaaacccggc cccgacgatg
    41041 atcgcatcca cactcatggt tctctccttg cgccccgcgg gcggcttcgc cttggcgaat
    41101 tcgtcaatac gccccgtggc cgctcaccac cgtgcggatc gtcttgcaca ggatggcgat
    41161 gtcgtaccac aggttccagt tcttcacgta ccacgcatcc agcgcgacgc gctcgtcgta
    41221 ggtcgtctcg ttgcggcccg agacctgcca caggccggtg aggcccggcc gcgcctcgag
    41281 atagaaggcc acgcgctcgc cgtagcgttc cagctcggcc tcgaccaccg gccgcggccc
    41341 caccaggctc atctcaccct tcagcacgtt ccacagttgc gggatctcgt ccaggctaga
    41401 gcgccgcaga aaggccccga tgggggtgat gcgcggatcg ttcttcagct tgaaatcccg
    41461 gttccactcc tcccgcgcct ggggatcgga cgccagtagg cgcgccagca cctcggccga
    41521 atcgtgggtc atggtacgga acttccagca cttgaagggc accccgtcca ggccaatgcg
    41581 ctcgtggccg aagaacaccg aacccggccc gccctgacgc ttcacctgca ccgcgatata
    41641 ggccagcagc ggcgacagga gcgccagtcc gcaggccgac ccggcgatgt cgaacatccg
    41701 cttgagccag cgcagcgggc gagacgccag gttgttgcgc acccgcagca tcagcacctc
    41761 gtggctgaag aagtggttgg cgctcagccc gaacaaaggc agcccggcaa tcggcgggat
    41821 gatgtgcaga tccttgtaat gcagggtcag cagctccacc tgcccggcga tttcctgcag
    41881 ttccggctcg ctcaccgcca ccgtcacatg gggatgtccc tgatccgcca gccagggcag
    41941 gagcttgtcc gggcgcgcca ccggtaccgt gacgccaggc gccacgacca gctcggccgg
    42001 cgcctcgggc ccgacggcga cgaaggcgtg cacctcgaag cccagcagat gctcgctgcg
    42061 cagggcctgc caggcggcca cggcgttctc accgacgccc acgatgacgg tcggcagttg
    42121 ccaggagccg gcccgcatca gcgcggactt catgccgtta cggaacaggg gcagcagcac
    42181 cagcgccagg ccccaggata ccggccacag gaagcgcgac accggccact tggccagcag
    42241 caccaccatg ccgttgagca gcgccgccag cagcaggctg cggatgatct ccagcaactc
    42301 gtcccagaag ggttgtcgcc ggtggtaatg cccacgccac gcgaaggtga cgagcgccag
    42361 caccagcagc aacaggaagg ccagcgcctg gttcacccct tccgccgccc accactgcgc
    42421 aaaactcggt ccgatgcgat gcccgcgcaa ccacagcagc aggagcagcg ccaagccgaa
    42481 ggccaccccc aaggccagcg catcggccag caccagccag ccgcgcactt ccccggcggg
    42541 ccgctgcggg cgcctcatgt cagttctgga gtccataggc ccggtacagt gcctccatca
    42601 tccggtcgaa ggcgaaatgc gcgcgcacga aggcctgggc ccgtgccccg gtcgcctggc
    42661 gcagggccgg atcgttcacc agcgggccga gccgcgcgac gaaggccgcc gcgtcctccg
    42721 gcaccagcca gcccgtgtcc ccgtcctgca ccagatcccg gttgcccacc acccgcgtgg
    42781 cgatcaccgg cagccccgcg cccatggcct cgatcaccgc caccggcaac ccttcccagc
    42841 gggacgtctg cagatagatg tcgagccggc cgagctcctc cagcgcacgg gcccggtcca
    42901 cccagccggt gatgtgcacg cctgccgccc gcagcgccgc ctcgccggac gcctcgccgc
    42961 cgccgatcca gacgaactcc acgccgtcac gtccgctgcg gcgggccact tcggcgaaga
    43021 gctccggatg gcgcgccgtg gtgacgcgcc ccaccgtgcc gatgcgcacg acgcctgtcc
    43081 cgccccgcgc ctggggcagg atcgccaggt ccaccgcatt gggcaccacc accacgcgcc
    43141 ggctcagatg ggtgcggatc tgctccgcct ccgagggcga acaggcgatg aaggtcaccg
    43201 gaatgcgcgc cagcagcttc tcgagggtca ggaacaccgc gttcttgatg cgccccgccg
    43261 ccttctgcag gaaggacagc ccatggggcg aaaaaaacca gcgcggcccc ggacagacca
    43321 ggctcagcag gcgccccagc gcaccggcct tggacgagtg cagatgcacc acgtcgggcc
    43381 gccagtcccg caggatgcgc cgcagggcca ccgccgacgc ccagtccgtc gccgggcgga
    43441 tcgcccgggt catgggcaca tgacgggtcg cgatgcgcgg atcgaagagc gcccgccagt
    43501 tcgccggcgt ctcttcccgc gtcgaataca ccagcgacac ccggtgcccg tccgccgcct
    43561 ggcggttggc gatggccgac accatggaca gggtgccggc accgaaggat tccaccacgt
    43621 gcaggatatg cgccacgatc ccctcaaccc gcccgcgcgc cccggcgcag ctgggacttc
    43681 agggcgaaca cccaatccca gaatgcccgt tgcgcagcgc actcatcgat gggtacctga
    43741 acgctgccgt ccaccaccac ttcgcagcct tcaccgccgt caatgggtgc ttgggaacat
    43801 atcaatctca gacgaatttt gtaatgggga atcaggtaga cgaggaaatt cacgctcaag
    43861 actcaggagt gcgccgccgc aacggccgat tcaacagccc cgatccgaaa cgcagacggg
    43921 agacgcatag acgggaaatg ggtccacatc ggcgagccgg gcagcccacg ccccatggcc
    43981 ggaccgggaa ggccacgcac ggcgcaaggg gttgatatcg caccgcatct tactcgaaaa
    44041 aggctcgtga ccgcgatggg cggctgcggc taggcctgcc cacgcgcccg ttggcaggcc
    44101 gccgccgtga cgacgcccgg tgctatcatt cagcggtttt ggcgccggcc ggcgcattcg
    44161 acggcccgcc gcatccttcc gcgccccgac cccgcgaata tccgcgcccg cagggcagga
    44221 aggttcaccg ccacggcccg cttcatgaat gccctgccct ccgtctcccg cccgttttcc
    44281 gaccgcgccg aaaccgtcgc cctgtgcctg atcgtcgtct gcgccttcgt cgtccccctg
    44341 cccacggcgc tgatcagcat ctgcaccttc ctgttcctgc tggcctggct ggcggccggc
    44401 cggctgggcg agcgctgggc caccctacac cgccatcccc agggtcccct gtgtctcgca
    44461 ctgctcacct ggatgggcgt ctccatcgct tggagcccgg cccctttcca cgaagccctc
    44521 gataactggt ggcacttccg cgaatacatc ctgttcctgc tgatgctcac cgtcgtcgcc
    44581 agggaaccga aaaaatggga acggcgcatc ctgctcggct acctggccgg ctactgcgta
    44641 tccctgctca cctcctacct gcgctggttc gaggtgattc ctctctacga ccaggtgggt
    44701 gaatttgccg gtttcggcgg ccacacgggc ttcagcctga tgctcgcgat cacgtcctac
    44761 gcctgcatct ggctgtggcg ggcgcgtccc ggcctgcggg tgttctggct gctgctgatg
    44821 atcctgtccc tgttcaacct gtatttcatc aacactggcc gcaccggtca ggtggccttc
    44881 ctggcgctga ttcccctcat cgtgttccgc tggatgggcc tgccggggct gatcgccggc
    44941 gccctcggcg cggtggcctt cgccggttcg ctctacttcc tgtcgcccac ggtgcattac
    45001 cgggtgaaca acgccgtcta ccagatcgag cagtaccggg ccggcaacac caccaccagc
    45061 gacggcctgc gcatcgagtt ctgggtcaac gcggtcaaac tcatccgcca ccatccgatc
    45121 ctcggcggtg gctccggcag cttcgcggcc gaatacgaag tcctcgccgc agagcagcac
    45181 atgaccggcg accacgtggc ggtcaatccc cacaacgaat acctgctcat gtgggcccag
    45241 tacggcctgc ccggcctcgg cctgttcctg gcactgtggc tcagccagtg gcgccgcgcc
    45301 tgccgcgaag cggaaacccg gcgctacctc ttccacggcc tgatcctcgc catggcggtg
    45361 ggcgatctct tcaattccct gatcctcgat aacatggaag gccacttcta catgctggtc
    45421 agcgtggccc tgggcgccgc ctgggcccgc gacgaccgcg ccggcggccg ctaaaggcgc
    45481 gcggatccgg cgcggcggcc cgcccgtttc cgctacactc gggccatccc gtccttccga
    45541 gaccgccagc atgtcctacg cgccctacat caaggagatc ggccgcgggc cgaaaggctc
    45601 ccgtcccctc accgtcgacc aggccgaaca gctcttcggc gacatgctcg acggccgcgt
    45661 ccccgacctg gaactgggcg ccatcatcct gtccatgcgc atcaaggccg agtccgtcga
    45721 cgagctgttc ggcttccagc gcgccctgga cgcccgcacg gcccacctcg ccgtgccgcc
    45781 cggaccacgc ctggtggtgc tgcccaccta caatggcgcc cgccgccagg ccaacctgat
    45841 gccccttctc gcccgcctgc tggcccgcga gggcgtgccg gtcctgatcc agggccgcca
    45901 cgatttcgaa tcccgcgtca gccctttcga actgctcgcc gccctggaca tccacccggc
    45961 gaccagcctg tccgacgcca ccgatcgcct cgccagggat cgccttgcct gcgtaccgct
    46021 cgacctgctc gcacccggcc tcgtgccgct cctcgccacc cgtgtgcgcc tgggcgtccg
    46081 caacagcgcc cacaccctcg ccaaactcct cgatcccgcc cccggccaca gcgtgcgggt
    46141 cgcctccatg acccatcctg agtacctgga gcgcatccac gcccacctgg tggccaccgg
    46201 cgccacggcg atgctgctgc gcggcaccga aggcgaggcc ttcgccaacc cccgccgccg
    46261 cccgctgctg gagcgctttg ccaacggtgt cgccgacgtg gcttttcccg ccgaggaagg
    46321 cggcgcccct ccgctgcccg gcgtcaccga caaccctgag gtggccgcca acgccgagct
    46381 catccgcgcc atgctggcgg gcgccgtgcc gatcccctcg ccgctcctcg accaggtcgc
    46441 ggccttgcgg gatctcgcac acctggcgcg cgcctgaggg gcatgggcgc actgttcgcc
    46501 catgtcacct gcggccggac aagcgtctat actgggatct ccccggcata ggacggtgtc
    46561 gccccgatgc tgccggttgc cgaccctcac ccctggagac gcaccatgag caagaaggcc
    46621 ctctgcatcg gcatcaacga ctaccccggc accggcagcg acctctccgg ctgcgtcaac
    46681 gacgccaacg actgggccac gacccttgcc ggccgcggtt tcaccgtcac caagctgctc
    46741 gacgcccagg ccaccaaggc cgccatggtg agcgccatga gcagtctcat cgccggcgcc
    46801 gaaccgaaca gcaccgtggt catcacctat tccggccacg gcacctgggt acccgaccag
    46861 aacggcgatg aagccgatgg tcgtgacgaa ggcctgtgtc cctgggacat tgcccagggc
    46921 aaggtgctca tcgacgacga aatcgccatc ctcttcgcca agcgcgccaa gggtgtgcgc
    46981 gtgctgctca tcgccgacag ctgccactcc ggctcggtga cccgtgcccc ggaggacatt
    47041 cccgaacccg gcgaaccgaa gccccgcttc ctgcccctct ccctgtggct caagaccggc
    47101 tacaacccgc ccgtcgtgcc gcccaagcca cccgccggca cgctgggcct cgaccgggcc
    47161 ggtggtgaca ccctgctggc cggctgcaag gacgaggagt tcagctggga cacctccttc
    47221 gctggccgcc ccaacggcgc cttcacctac tacgccctca aggctctggg cagcctgccg
    47281 gccaacgcca cctacgccga ctggttcaag aagatccgga cctacctgcc gtccagcgcc
    47341 tgcccgcaga acccgcagat cttcgggccg gacggcgccg acgccttccc catcttctcc
    47401 tgacctcccg cgggacggct gcgaccgtcc cgtacgcatc gatcctccct gcccgaggaa
    47461 tccgccatgc cccgtaccta ccggatccgc ggcgcccagg acgcatcgcg tcccgcggtc
    47521 gccctgccca ttgccgacac ggccgccatc tccgtcacgc atgtgggcca cctcctgccc
    47581 ggcactgccc gggacgccgc cccgaacgaa ctcgacatcc cggccgacac ggtggtgcgg
    47641 gtcaccctcg acaacggttt cgtgctgtgg tcacgagccg atgacctgat ccgtgaatac
    47701 ggccgcaagg cccttgcccg ggacggcggc gaaggctggg aattcggtcc cctggtgcct
    47761 ccccgccggg gcgccggcga acgcggcctg ctgggactgg gcatcaaggt gctggacttc
    47821 ttcggcatcc acgtgatgaa caagcctgcg gcggcgatcg cggcatggct cgaggacaag
    47881 cgcctcggcg gccatgcgcc gggcgtgtac cgctgccccc tcgacggccc cttcgccctg
    47941 tcgccccttg gcacaaagga gacccttgcg gccgcaccgg gtccgagcct ggtcttcctc
    48001 cacggcacgg gctccagcac acaaggcagc ttcagcgaac tgtgggatcc caaaaatgcc
    48061 agtggtgccg tcctgcgggc ccgtctgaag ggcgtctacg gtgaccgtgt ctatgccctg
    48121 gagcaccgct ccctcaccga aagtccgatc cagaacgccc tgcagctcgt ggagagactc
    48181 ccggaaggca ccgagctgca tctggtcagc cactcccgcg gcgggctcat cggcgagctg
    48241 ctgtgcctct ccggcgtaga cgccctcgcc gaatccctct cgccgaccac cctcgacgcc
    48301 ctcttcaatc cggaccggat catcgccaga cagctcggcc tcggccccct gggcgaacag
    48361 gccctcgccg accgcaaggc ggcctatgac gctgaacgcc gacgcctgag cgacctggtg
    48421 ggcctgctcg ccgccaggaa gatacgcgtc tcccgtttcg tccgcgtcgc ctgcccggcg
    48481 cgggggacca cgctggcgtc cggacgcctc gaccgctggc tgtccaccct cgacttcctg
    48541 gccgggcgtg tcctcggcga cggactcttc atcggcggac tcgacttcgt gctggccgtc
    48601 gtccacgagc gcaccgaccc tcgcacgctg ccgggtctgg aagccatgat gccgggctcc
    48661 gccctgacca gcctgctcgc ggcccccggc ctgaccaccc gcgccgacct caccgtcatc
    48721 gcgggcgaca tcgaaggcga ttcgctgctg cagaacctca agctgatggc ggtggactgg
    48781 ttctacgccg ccgatcacga cctggtggtc aataccggct ccatgtccgg cggactcaaa
    48841 cgtccgcccg gcggagcccg ctaccgcctc gaccggggca gcgacgtcaa tcacttcgcc
    48901 tacttccaga acaagaagag cgtcgaatgg ctgctcgccg gcctcacccg cgccgatggc
    48961 gccgacggcg gcttcctgac catcgccagc gccccggccg aagccccccg ctggcgtgac
    49021 gccctggccc gcagcgccac cgtgagcgcg ccgcggcccc tcgccgtggt cctgccgggc
    49081 accatgggca gccacctcag cgtcgcgggc gaccatgtct ggctgaatta cggggccctg
    49141 ctcctgggcg gcctcaagaa gctgcgccgg gacgcaaacg gcgtcacccc caccgagctg
    49201 atcgaccagt tctacggccc cctgctggag tttctcgccc gcagccaccg ggtcgaatac
    49261 gtcgcttacg actggcgcct gtccatccgg gaggccgccg gtcgcctcgc cgacaccctg
    49321 gagcgctgcc tgcctcaggc cgaacgggac aaccagccgg ttcatctggt cgcccactcc
    49381 atgggcgggc tggtggtccg ctgcatgatc gccgacggcg cccgcggcgc ctctctgtgg
    49441 caacgcatca cacgcctgcc gggcagccgc ttcctgatgc tcggcacacc caacttcgga
    49501 tcctacgaag ccatgcgctg gctcactgga ttcaatccca ccgaggccaa gctgtccctg
    49561 ctcgacttca cccagagcac cgacgacatc gtcaacctgg tgcgccgcta ccccggcctg
    49621 ctggaactgt tgccctttgg cacccaggac ccggatttct ccaccgtcgc cccgtggcag
    49681 tccatcaagg acaagaccct cgcagcctgg gacgtccccc agagcgacga cctgcgcatc
    49741 gccgcggcca cctggcagtg cctgcgcaat gcagcaccag acccccgctt catgcgctac
    49801 gtggccggct cccaagccgc caccgtcacc gactaccagc tcctcgccga cgacgacctg
    49861 accaatcccc gccagcacct ggacttcatt gccacgcgcc gcggcgatgg caccgtcacc
    49921 tgggcgtcgg gcaccctgcc cggcgtgccc atgtggttcg tggacgatac gccccacgat
    49981 gccctctgca gcgccacgca ggccttcccg gcctatctgg aaatccttgc cagcggcacg
    50041 acgaaccgcc tcccgtccgt cgcggacgcg gtcagccgcg gcgacgcgcc ggacgaacgc
    50101 ttcccgctgc ccgccacgcc tcccgaggac ggcattcccg acgaatccgg cctgcgcggc
    50161 ttcggcttcg gcggcggcct gcccgcagag accccgaacg cagcccctgc cgccccggtc
    50221 atcgaggtca gcatccgcca cggtgatctg tcctacgccc gcttcccggt tctcgtgggc
    50281 cattaccagg gcgacccgct ggtccacgcc gaagccaccc tcgaccgcca gctcgacggc
    50341 gccctgagcc gccgggtcaa tctggacatc taccccggac gtctcgacag tcacgccgtc
    50401 ttcttccgcg agaacccccg cctcaagccc accggcgccc tcgtcatcgg cctcggccag
    50461 gtgggcgccc tcacgcccgg cctgctggaa cagatcgcga ccccggcgct cctcgattac
    50521 gcgctgcagg cggcccgctg gccggacgaa cgcttcgggc cggccagcgc gccccgctcg
    50581 gccgcggtct cctgcctgct ggtgggctcc ggcgccggag gattcccggt gcgcgacagt
    50641 atcgaagccc tgctgcgcgc cgccgtgacg gtcaaccgcc gcctggtgga cgcccgcctc
    50701 gaaggccgga tcaccctcga caagctggaa ttcctcgaac tcttcgagga tgtcgccatc
    50761 ggcgccgccg aagccctgaa ccagattctg gccgacggcg agttcgccgc ggccgtcagc
    50821 tggccccagc gatgcatcga acccggtcag ggcggtcgcc gccggatccg cttcggcgac
    50881 gcccccggct ggtggcaccg ccttgacatc gcccgtgacg gcgagcgcct gcgcttcacc
    50941 ttcgccaccg atcgggcccg cgccgaggag accctcgcga ccggtcagct cgccctggcc
    51001 gacgccttca tccggcaggc ctcgcgctcg ccccgggcca atccggaggc ggcccgcacc
    51061 ctgttcgagc tgctcctgcc ccttcgcctg cgggagatgg cgccccagca gggcgacatg
    51121 gtcctgctcg tcgatgagga ttcggcacgc tttccgtggg agctgctgga agaccgctgg
    51181 agccacagcg ggcgcccgcc cgcggtccag gccgggctgc tgcgccagct caagaccgcc
    51241 accttccgag cgcgccccag ccttggcagc gaagcacgcg cgctggtcat cggcaacccg
    51301 gacctgagcg gcagcaccgt cttcagcgac ctgcccgggg cacgcgacga agcgacccgc
    51361 gttgccggac ttctgggcac gcgcggcttc gaaacggaag actgcatcga cgccaaggcc
    51421 accagcatcg ttgaaaccct gcacaagcgg ccgtggcgga tccttcacct cgccggccat
    51481 ggcgtccatc gcttcatgat cgccggcgac acgccgcggc cctgctccac ctgcggacag
    51541 gccacgccgg ccccacagaa accgatctcc ggaatggtca tcggagacgg catcttcctc
    51601 acgcccggcg acgtggagca gttgcgctgg gtccccgaac tggtcttcat caattgctgc
    51661 cacctgggga acaccaccgc gcccgcggac gatctggacc gcgccggcct cgccgccaat
    51721 ctgggggtcg agttcatccg catgggcgtg cgcgccgtca ttgccgcagg ctgggcggtg
    51781 gacgatcggg cggcgtccac cttcgccgaa accttctatg gacgcatgct cgccggcgaa
    51841 agcttcggcg atgccgtgcg tggcgcacgg gagcaatgct ggatcagcca tccgggcgta
    51901 aacacctggg gcgcgtatca atgctatggc gacccggcct atcgcctgag acccgcggtc
    51961 gccaccaccg agacagggca cacgcccttc tttgccccgt ctgaactggt ggccgaactg
    52021 agcaacctca tcggccagct caaggcccac gtggacccgg acagcccgcg catcatcgag
    52081 gccatcctgc aacgcattcc tgccgcccaa ggcgcatcct ggcgtgcccg cgccgacgtg
    52141 agcgcggccc tcgggctggc ctggagcgaa gcccgctgct ggccggaagc catcgccgcg
    52201 ctggaacagg cgatgcaggc caacagcgcc gacctgccgg tgcgcgccat cgagcaatgg
    52261 gccaacctga gcgcccgccg agccgtcgag tgcctccatg gccgtgcgcc gatcaccgct
    52321 gcgcagcgca aggagatggt cgccaccctc aggaccgtga tcgaccgcct gcagtccctg
    52381 cgcaccttcg gcgccaccat cgaacgcctc agcctgctcg ggagcacctg gaaacgcctc
    52441 gccctggtgc agacgggaag cgcccgcgtc gcggccctcg ggcacatggc agacgcctat
    52501 ggcgaggcct ttgccctcgg caaagagacc gacccctatc cctttgcaaa tgcctggacg
    52561 gccgaactgc tccagcccga tgcgccgtcc cggcctgaac gggacacgac ctgcagccgc
    52621 ctgctcgccc tgcaggcaga cgccaacgcc cgcaacccca gcttctgggg cgacatcgcg
    52681 gtggccgatc tgaagctcgc ccgactcctg gcccgctgcg caggcggcgg gcccgccccg
    52741 agactcccgg agacatgcct cgagctgagc caggaagtca ccgaagccta tcaggccgcc
    52801 cttgcccggg gcgccagcga acgggagcgc gcctccgtga cggaacacat cgatttcctg
    52861 atcagactga ccgcaccgaa atcgccccag cttgccttcc tccagacact ccgcaatacc
    52921 ctcgcctgag cgccgtcatc agccgtctcc gtccggtacg cgcccggcgg agacggcaac
    52981 accccgcctc tccatgcacc gcaagggtgc ccgcccctgc gtgcaccgca ccatgaagga
    53041 tcatttcgcc ccgcaacagt gcgcacagtg gcaaagacac tgctgttttt tcagcgaaaa
    53101 cttttggcat aagcattgct tcagctccgt gacaaggagc tcgcatggct gaaaccaaat
    53161 ccacctgttg ttactgcggc gtcggttgcg gcgtcatcat cgaacacgac gccggacgca
    53221 tcaccggcgt acggggagac cccgaccacc cggccaactt cggccgcctg tgcatcaagg
    53281 gctccacgct gcacctcacg gcggcacctg aaaccctcgc cacgcgcgcc ctccatcccg
    53341 agctccggac cggccgcgcg ctgccccgcc agcgcgtcag ctgggacacc gccctcgaca
    53401 ccgcggccga acgctttgcg aagatcatcg ccgaacacgg ccccgacgcg gtcgccgtgt
    53461 acggcgccgg ccagctcctc accgaggact actacgtctt caacaagctg gtgaaaggcc
    53521 tgatcggcac caacaacctg gacaccaact cgcgcctgtg catgtccagt gcggtggccg
    53581 gctacaagct cagcctgggt gccgacgcgc cgccgtgcag ctacgaggac atcgcccaca
    53641 ccagcaccct cttcatcacc ggcagcaaca ccgccttcgc ccaccccatc gtcttccgcc
    53701 gcgtcgagga tgcccgcaag gccaaccccg acatgaagct ggtggtgatc gacccccgcc
    53761 gcaccgacac cgccgaaggg gcggacctgc atctggccct gctgcccggc accgacatcg
    53821 ccctctataa cgcgatgctc cacgtgatgc tgtgggaagg ctgggtgaat cgtgactaca
    53881 tcgacgcgca caccgaaggc ttcgacgccc tcaagcgcac cgtccgcgaa tacacacccg
    53941 ccatggccgc caccatctgc ggtctggaca agaaggtcat cgaacaggcc gccgaactct
    54001 tcgccacctc cccggccacc ctgtccatgt actgccaggg gctcaaccag ttctccttcg
    54061 gcacccacaa caactcggcg ctgatcaacc tgcacctggc caccggccag atcgggcgtc
    54121 ccggcgccgg ccccttctcc ctcaccggtc agcccaatgc catgggcggc cgcgaagtcg
    54181 gcggtctggc caatctgctg tcggcccacc gggacatggc caacccgcag caccgcgccg
    54241 aagtggccgc cctgtggggc gtggacagcg tgccggacaa gcctggcaag agcgccgtgg
    54301 agctgttccg cgccctcaag agcggtgaag tcaaggctgt ctggatcgcc tgcaccaacc
    54361 cggcccagtc cctgcctgac cagaccctgg tccatgaagc cctgcagagc gccgaattcg
    54421 tcatcgtgca ggaagccttc ggccacaccg agacctgcgc ctacgccgat ctcctcctgc
    54481 ctgccagcac ctggggcgag aaggacggca ccgtcaccaa ctccgaccgg accatctccc
    54541 gcgtccggcc cgccgtgtcc gcgccgggcg aagcccgcca cgattgggcg atcgtggtgg
    54601 atttcgcccg gcgtctgggc acccgcctcg gcaagggcga ccttgccgcg cggatgttcc
    54661 cctacgaccg gcccgaagac gtctggaacg agcaccgcga atccacccgc ggtcgcgatc
    54721 tggacatcac cggcctcagc tacaccacgc tggaagacgt cggtccccag cagtggccct
    54781 tcatcgaagg cgcccaggct ggcaaagtgc gcctctacgc ggacggcgtg ttccccacgc
    54841 catccggcaa ggctcgcttt gtcgccaccg aataccgagt caccgccgag gcgcccagcg
    54901 cccgcttccc gctgcacctg aacaccggcc gcctgcgcga ccagtggcac ggcatgagtc
    54961 gtaccggcct ggtggcccgc ctctacagtc acgaatccga acccctgctg cacatgcacg
    55021 ccgacgatct cgcccgccgc cagctgagca gcggcgatct ggtccgtgtg aagagccgcc
    55081 gcggcgcgat tgtcgtcaag gttctggaat ccgacaccgt gcgtccgggg caatgcttcc
    55141 tgcccatgca ctggggcgcc aacgccatgg gcggacaggg cgtgaacgtc ctcatgccgc
    55201 ccgacttcga tccgacctcc aagcagcccg agctcaagca tgccgccgtg caggtggaga
    55261 aattcaccga aggcaaggcc ctcgtggcct tgcgccgctc cgaaccggat gcgaacggcg
    55321 tcctgcagcc gaatccgctc gaagtcatgg agagcctgcg ccgctggctg ccgcacttcc
    55381 cctatgccag cctgaccctg gccggacgcc atgagccggt cgtcgtgctc aaggcccgcg
    55441 gcgaggaact cgcgccggaa ctcctcgagg ccctggaccg ggatctgagc ctcgacgatc
    55501 cgacccgcgt cctgtcctat caggatcgtc gcaaggacgt ggccaagcgg gcactggtgg
    55561 agggcgactc cctcacagcc gtccgtctgg cgggcgagac ccgcgcggca gactggctcg
    55621 ccgacatgat ggtgcgtggc cactcggccg ccgcggtgcg cccgtggctg ctcgccccgg
    55681 tggatcagcc gccagccggc cagtcggcgc gtggcaaggt catctgcaac tgcttcgacg
    55741 tatcggaaga ggacatcgtg gccgccttca agaatggcga caccctcgac gccctccagg
    55801 cccgcacccg ctgcggcacc aactgcggct cctgcctgcc cgagctcaag cgacttcagc
    55861 agcagaacag ccactgagcc gggcctcgcg caccccacga aaaaaccggg cctgaaggcc
    55921 cggttttcat tgcccaaccc gggcgcctag gacgccggcg cccacccctg ggccaccagc
    55981 cactcaaagg tcgcggcgga caccgcgggg caatcgtccg gcccggaaaa tgtgtgatca
    56041 gcctgttcaa aatgccgcgt ggacacctcg ggccgtgtca cgacggcttg ccagcgttcg
    56101 ctgccgtcac gcacctgctc gaattcccgc gcaacgtaat cccgaccgct gaggaatagc
    56161 gcggccggcg tcccggcgcg tcgcagcatg ccctgcgcca tgcgctcggg caggctgccg
    56221 gcgtcggcct catccgacga attcgccgtc ctggcggccc cgtcaccgcg cgccgcccgc
    56281 gcggtggaaa acagggaccg gatcgatccg aacacattga cgccgccccc cagcagcttc
    56341 ttccagaagg acggatcgga cagacgctgc aggtaatagt gcttcaggaa caccttggcc
    56401 tgccccgcac tggtctgcac ccaggggttg aggatcacca cgccggccac ccgcgcatcg
    56461 ctcgccgggt agacgcacac tgcgctggcc ccgtcacaca gaccccacag gacgaccttc
    56521 tcgagccccg gttgggccgc aagaaaggcg tccatggcgg cccggatgtc ctcgctcacg
    56581 tccaggaagt ggcgcgccgc gccgctggca tcgcccatcc cgcgataatc gaagcgcaga
    56641 cagggcaccc cgcgcgccgc cagatagcgc gccagactga cgaactggcg atggctgccg
    56701 acccggtact ggggcccgcc gaccacgacc agcaccccga cgcgagccgg cccctgcggc
    56761 gccgaaagga tcccgaccag agactctccg ccgcactcga agacgagcgc ccgatcctgc
    56821 cagttcatcg cacgaacccc ttcaacgcct cgaccgaggc ctcgataagc ggctcgcaga
    56881 cttcgatttc ctgggtctgc cagaaggcaa cacccggcac caccgccgca cgcgctgccg
    56941 tccccgcgtt cgcccagcga tcgaccggca cccgcagcgc gggtgacacc tcctgacgat
    57001 ccgctggcgc cacctccagc gccaccaccg gcgccgcata cgcggctggc aacgacagcg
    57061 tcgccgcctc catgccggag gccagtgcgg ccggcaggcg atacccggca acatccacca
    57121 cgccgccggc ctttaatgtg gcgcgaatgg cgtccatgga agccttggcg tcggcatcgg
    57181 cgagcatgtc gaaggcggcc ttcaggcgca ggaactgggt caggtgctgc ttgccgttgc
    57241 cgaccggctg ccacagcagc agcggtggcg ccgtgccgtc cgccttgatc cagtcggaca
    57301 ccaccagggc cccgagacgc atgccccaca gggccaccgg cacccgcagg tctgtcgtcg
    57361 cccagcgcac ggcgtcgcgc agatcgtcga tccacgtacc ccatgacaca tcggcaagct
    57421 cacccccgct gtcgccgcag ccggacagat ccatttgcag gacgttccag ccccgctcgg
    57481 cgaaggcgcg cgccgcgagc gccaccatcc gccgggactt gttcatttcc tcggcgaagg
    57541 cgtgcacgaa tacaagggat cctaccggct cgggcgcgcg cgtaaggagg cagaatcgtc
    57601 ctccgtgcgc agcccccggg aggaagaacg cctcccgccg caacggctgc acgccctgac
    57661 ctcaggcgag cttggcgtcg acgaactcca ccagcgagcc gacggaggcg aaggtagcgc
    57721 cgtcgatctc atcgtcttcc acgacaaagc cgaaacgctc ttcgagcatg ttgatcaggc
    57781 ccacgacggc catggagtcc agatcgggca gagcccccag cagcggcgta tcgagatcaa
    57841 actccgccgc acgccctccc aggctcaaca cctcgtcgag aatcgacagc acttccttct
    57901 tggtatccaa aagcagactc cgtagcaaaa ctaaatcaca tcgcagcggc cgggccgagc
    57961 gacggcgtca tggcaagact gctatcatta tattcgacac tgcggtcctc gggaccgtga
    58021 ttgaagttgc aggccgcgtc ggcggcattt tccggttcgg ccggtttttt tttgcccagc
    58081 gatgaaacgc gttctcctga tcgccttcca ctttccaccc gtccagggca gcagcggggt
    58141 acagcggacc ctcaggtttg cgcgctacct gcccgatttc ggctgggaac ccatcgttct
    58201 cacggcccac ccgcgcgcct acgcggagac ctccgacgat cagctcaagg acattccccc
    58261 cggggtacgc gtcatccgcg cccccgcctg ggacagctcc cgccacctgt ccatcgccgg
    58321 ccgctatccg ggcttcctgg cgcgacccga ccgatggatg tcctggtggc tcggcgcggt
    58381 accggccggc ctgaaaatca tccgtgaatt caatccactg gcgatctggt ccacctaccc
    58441 catcgccacc gcccaccgca tcggcggcag cctcgcgcgc ctcagcgccc tgccctggat
    58501 cgccgacttt cgcgatccca tggtggacgg cgactacccg tccgatccgg ccatccggcg
    58561 cagtttcgag gccatccagg gcaacgccat ccgccgcgcg gcgtgcagca ccttcaccac
    58621 ccccagcgcc ctggccatgt tccgccgcca gtttcccgag cgggcgaacc acatggccct
    58681 gctcgaaaac ggctacgacg aagagacctt cgccggccag accgccaccg gacgcctcca
    58741 tcccgaacgc ctcacgctgc tccacagtgg cgtgatctac ccctccgaac gggatccgac
    58801 acaattgttt gccgccctgc gcctgctcaa ggagcgcgca ccggccgtcc tcgcccgcct
    58861 cggcgtgcgc ttccgggcgc ccatccattt cgacctgctc gccgatctgg cgcggaccta
    58921 cggcgtcgag gaacacgtgg agctcgccca gcccgtaccc tatcgcgaag ccctggccga
    58981 gatgctcaac gccgacggcc tgctcatcct gcaggccgcc aactgcaacg cccaggtgcc
    59041 ggccaagctc tacgaatacc tgcgcgcgcg ccgcccggtc ctgaccctga ccgacccggc
    59101 cggcgacacc gcccaggtgg tccgcgacgc cggcatcggt gccctcgccc gtctggacga
    59161 cgcggccgac atcgccagcc tgctcgaacg cttcgtcgcc cagcccgaca ccggcactct
    59221 gcccaccgaa gccgccatcc gcggtgcctc ccggcgcggg cggaccgaag ccctcgccgg
    59281 cctcctcgac agggccgtcg cccacggcgg agcctgagcg atggcgctgg gcaagctgcg
    59341 cgccaccttt gccgagatgg gcgccctcaa cggcggtctc tatctcctcc accgcatcct
    59401 gcagaccctg agcggcggcc gcgcacagct ggtgcgctac tacctggtgg cacaaccgat
    59461 ccatccggac gccgcctgcc gcccgtcggc caagaacccg gtcacgctgc tcgacccttc
    59521 cgatccgctg attgcagcct ttccacgtcc gccggaggtc attgccaaac gcctgcggga
    59581 cggcgccacc tgcttcatcg cccgctccgg cgagaccttc gccggcttcc tgtggctcgc
    59641 ccatggcgcc tacgacgagg acgaggtgcg ctgccgctac gaactcgtgg acgacgaccg
    59701 ctgcgtgtgg gattacgacg tctacgtcga acccgcctac cgcatcggcc ggaccttcgc
    59761 gcgcctgtgg gatgcggcca accagcacct cgccccccgc ggcgtgcgct ggagcatctc
    59821 gcggatctcc gccttcaatg ccgcctcgct ggccgcccac gaccgcctcg ggatcaagat
    59881 tttgcacagt gccacgttca tctgcctcgg atccgtgcaa attagcgccc tcggcggcgc
    59941 gccctatctg catatcggca tgtcggacgc gtcccggccg gtcctgcgcc tgcgcgcacc
    60001 ggacgctgag taggccccgt caggcagttt ttcgcaattc cgccgcacag gctcggatac
    60061 actcaccgct tcattcaaag aacatcctct ggccctcacc gacccatgaa cgccaccacc
    60121 cttcttcacc agctcgtcac cgaatccgcc ttgcgggcgc ccgatgccgg cgcgctgcgt
    60181 gacggcgccg ccgaactgag ctacgaagcc cttgccggcc agatcggcgc ctttgcctcc
    60241 ggcatgatgg ccctcggcct ggatcgcacc gaacgggtcg gcgtgtacct ggacaagcgc
    60301 ttcgagaccg tcatcgcgtc ctttggcgcc gccgcggccg gtggcgtgtt cgtgccggtc
    60361 aatcccatcc tcaagcccca gcaggtcggc tacatcctgc aggactgcaa tgtacgggtg
    60421 ctggtcacct ccgtcgagcg cttcaaaccc ctcgccgata ccctcagcga ctgccacgac
    60481 ctgcgccacg tgatcctcac cggcaaggtg gacaccctgc ccgaactgcc cggcatgtcc
    60541 atccaccgct gggccgacgt gatggccgcg cccgcccgtg ccggtcaccg ggtgatcgac
    60601 aacgacatgc tggcgatcct gtacacctcc ggcagcaccg gccgtcccaa gggcgtcgtg
    60661 ctgtcccacc gcaacatggt ggccggtgcc aagagcgttg ccacctatct cgagaaccgc
    60721 gccgacgata ccctcctcgc cgccctgccc ctgtccttcg acgcgggctt ttcccagctc
    60781 accaccgcct tccacgccgg cgcccgggtc gtcctcctca actacctgat gccccgcgac
    60841 gtgctcaacg ccctcgtcaa gcacaaggtc accggcctca ctgccgtgcc gcccctgtgg
    60901 atccagctct cccaactcga gtggcccgag accgtcaccg accacctgcg ctacatcgcc
    60961 aacaccggcg gacgcatgcc gctggaaacc ctcaccgccc tgcgtgcccg tctgccgcgg
    61021 accaagccct acctcatgta tggcctgacc gaagccttcc gcgccaccta cctgccgcca
    61081 gaccaggtcg atgtgcgtcc cgactccatc ggcaaggcca tccccaactc cgacgtcttc
    61141 gtcctgcgcg aggacggcag cccctgtgaa ccgggcgaac ccggcgaact cgtgcagcgc
    61201 ggcgccctcg tcgccatggg ctactggaac gatccggaaa agaccgccga acgcttcaag
    61261 cccctgcccg tcaccgcacc gggccgtcag gccgggctgg tgctgcccga aatcgccgtg
    61321 ttctccggcg acaacgtgcg cagggacgag gaaggctacc tgtacttcat cgggcgccgc
    61381 gacgagatga tgaagacctc cggctaccgg gtcagtccca ccgaagtcga agagatcctc
    61441 tacggcacgc acctggtggg cgaatgcgtc gccttcggcg tgccccatcc cagcctcggc
    61501 caggccatct gcgtgatcgc cacgccgcgc gccggcagcg ccctcgattc cgccgccctg
    61561 ctcgccgaat gcaagcagcg catgcccgcc tacatggtgc cctcggtcat cgaagtgcgc
    61621 gacggcccgc tgccgcgcaa ccccaatggc aagatcgacc gcaagaccct gtccaccgaa
    61681 ttcgaaaacg cccgtaaggt ccagtcatga gcgacacccc ccgcgaaaag cccgtccacg
    61741 ccccgatgga ccagttcccg gtcgtgaacg gcgaactcac cgtgggcggc atttccctca
    61801 ctcgcctggc cgaccgggtt ggtcagaccc ccttctacgc ctacgaccgc gccctcctca
    61861 cccgccgcgt acaggacctg cgcgccacgc tgcccacggc catcaagctc cactacgcca
    61921 tgaaggccaa ccccatgccg gcggtggtgg atcacatggc gcgcctcgtg gatggccttg
    61981 acgtggcctc cgggcgcgag ctgcgtgtcg ccctcgacag cggcgccaat ccccatgaaa
    62041 tcagctttgc cggccccggc aagagccacc aggaactgcg ccaggccgtc gctgcgggca
    62101 tcctgatcaa catcgaatcc ttccgtgaag tggccgaact ggccgccatc cgccaggcca
    62161 ccggctggca tgtccgcgtg gccgtgcgcg tcaatccgga tttcgaactc aagagctccg
    62221 gcatgaagat gggcggtggc cccaagcagt tcggcgtgga cgccgagcag gtgccggaac
    62281 tcctcgccga aatcggccgc tccggcctgg ccttcgaggg cttccacctc ttcgccggat
    62341 cccagaatct gcgcgccgaa tcgatcgtcg aggcccagcg caagtgcttc gacctggccc
    62401 tgcgcctggc cgaatccgca ccgtcgccgg tcaggttcct caacctgggc ggcggcttcg
    62461 gcattcctta ttttccgggc gaacaacgcc tcgacctggc ccccatcgcc gccaacctgg
    62521 cgatcatcgc cgaggaagcc gcggtcaaac tgcccggcgc cgagctggtc atcgaactgg
    62581 ggcgttattt cgtgggtgag gccgggttgt acgtctgcaa ggtggtggac cgcaaggtct
    62641 cccgtggcca cacctacctg gtcaccgacg gcggcctgca tcaccacctg tccgcctccg
    62701 gcaacttcgg ccaggtcatc cgcaagaact acccggccgc cgtcggcaac cgcatggacg
    62761 ccccgctcgg cgaagcggtg tcggtggtcg gtccgctgtg cacgcctctc gacctgctgg
    62821 ccgaccggat ggcgctgccg gaagcccgtc cgggcgacct gatcgccctt taccagtccg
    62881 gcgcttacgg cgccaccgcc agcccgcggg gcttcctgag ccatccggat ctggtggagg
    62941 tgctggtctg acgtggcatc cgaactgcac cacgccgcac cgtctcgtgc cgcccgccgg
    63001 ccggctgaac gggttgcgga ccccggacca gccatggccg gtcctgcgcc cggtcgtgac
    63061 cggaagatcc cgatggatct gagttcttca accgccgtcc cccacgccga gccttctgcg
    63121 gaccgaaggc caccgcccac ccggtggcat cgggccacgg ccgcattcgc gcactgttga
    63181 gttgtcttcc aagatggacg ccaccgacgc ctccctgcct gcccccctca tcacgacgat
    63241 catccccacg atggcgtcaa aggcgcgggc cgcctcgctg aagcgggcga tcacctcgat
    63301 ccgcgcggca tcgaagcagc ccgttgccat catcgtggtg gtgaacggga cccgcagcga
    63361 tcccgaggcc tgcgcctggc tggccgatca acctgacgtg cgcctggagt tcgacgccaa
    63421 gccttctgcg gccaacgcca tgctgcgggg gcgcgaactg gtggcgacgc ccttcttcac
    63481 gtcgctcgac gacgacgacg aattcacccc gggcgcgctc gacctgcgcc tggccgcgat
    63541 gcagtccgac acctgccccg acatcgtggt ctgcaacggc ctgcgccgtg acagcacggg
    63601 cgacaccctc tgctacgacg atctgcccag cgtgccggcg gcgcccttgc cggccctgtt
    63661 ccgcatgcca tggctgtcca gctgtaattc cctgtaccgc acggccagca tcgatgcgca
    63721 tttcttccgc aacttccact cctttgccga gtggacctgg ctcgcctatc agttcggcgt
    63781 ggcgggcaag aagattgccg taatcgatac ccccacattc gtgtgcaacg acaccgcggg
    63841 ttccctctcg aaatcgaacg agtacgagat ggcctacctg tcgctgtacg aacggatgct
    63901 gagcctggca ccgccggctc cggtggcgga tctcatccgg cggcggatcg gctcattttg
    63961 gcacgaccaa tccgttactg ccctgcgcaa cggccagcgc ctcgacgccc tgcgccacca
    64021 cgtgcgcagc ctgcgccagc ccggcggcct gcgctacctg ggctactccc gttacctgat
    64081 ccccggctgg ccccagaacc gatgacggac tcctcttcca acagcgtctt caagggcgcc
    64141 ctcatgaccg tcctcatgcg ctggacggac cgtctcatcg gcttcatcag caccctgatc
    64201 ctggcacgcg tgttggtgcc cgaggatttc ggggtgatcg ccatggcgtc cctcgtggtc
    64261 gggctgctcg acgtgctgct ggatctgggg gtcaacgtcg ccctcatcca gaaccgaacg
    64321 ccgagtcagg ctcattacga ctccgcctgg accctgcggc ttgcccaggc tgccaccacc
    64381 gccctgctca tcgtcatcgc ctcacccttc gccggcgatt acttcaacga tccgcgcgtc
    64441 acgccggtgc tgcagttcat ggccctgagc ctcttcgttg ccggcctgga gaacatcggc
    64501 accgtcacct tccagaagga catgcgcttc gacctggaat accgcttcaa tttctccaag
    64561 cgcatcgtcg ccttcctggt caccgtcacc agtgcatggt tgctgcacag ctactgggcg
    64621 ctggtgttcg gcaccctttc cggacggatc ttcggcgtct tcctgagcta ccgcatggtg
    64681 gccatgcgtc cgcgcctgag caccgagaag atgcgggaga tcttcgccgt ctcccagtgg
    64741 atgctcgtga acagcatcgg catctacctg aacgccaatc tgcacaagat gttcgtcggg
    64801 cgcttcgcct ccaccggcac gatggggggg tacacgctgg ccgacgaaat ctccgcgatg
    64861 ccctccaccg aaatcctggc gcccctcaac cgcgtgctct tccccgcctt cgtgcgggcc
    64921 aagcacgacc tggtcgagct gaaacgggtc ttcctgctgg cccagggagt tcaatgcctg
    64981 atcgccgtgc cggccagcct gggcctggcc ttcatcgccc acgacgccgt gctggtgctg
    65041 ctgggcgaac gctggagcct cgcgattccc ttcgtgcagg tgctggccct ttccaacgtg
    65101 gtccaggcca tcaccaacag cggcgcctac atgctcctca ccctgggcaa ggtccgcagc
    65161 gtgaccctca ccacctgggc ccaggtcatc ctggtggcga tcggcgcgct gctcgtgctg
    65221 catcagccgg aagccctcga catcgccatc ctgcgtgtca gcgccgtctt cgccggactc
    65281 gtgctggcca tgtggatcct catgctcaac atgagcgggc tgcgcctgct cgaactggtg
    65341 cgcaccatcg tgcggcccat cctcgccacg gcggtgatgg gcggcgccat ccacgggctg
    65401 ggcgtgctca ccgccgatct cccggtggtc gtcgccatgt tcctcaagat ctttgccggc
    65461 atgttcagct acacggccgc ggtggtgctg atctggcgcc tggccggacg tcccgacggc
    65521 gccgaatcct atgtcttcga caagctcgcc ggcgtcctca ggcgccgcaa ggccgggacc
    65581 gcctgacccc tcttccccat tcctggaacg cactgactta cgaggtttgt tttggacaac
    65641 cgattctcca ttcaggggcg cctcagcccg aatccggccg gcacgccggg cgaactgtcc
    65701 atcgagcgcc tggacgacgg gtggcgctgc ctctgggccg gcgcccgcct ggaggtccat
    65761 gcccctgccc gtctggcgga gcgggacggc atcctgtgcg tggccagcgg atcgccccgc
    65821 ctgccggtca gcggcgccga catcgccgac gcctggctcg aactccacgc ccgccatggt
    65881 gccgatgtcc atcgcgacgt gaagggcctg ttcagcgtcg cgttctttga cacccgccgc
    65941 cagaccgcat ccttttacac cgaccgcttt gccaccgaat cctggtgctt cggtgaagaa
    66001 gacggcgccc tgtgcttttc ggaccgggcc gacgcggtgc ccctgacgcg tcgcagcgtc
    66061 agtgcccagt cgatcttcga ctacctgttc ttccacgtca tcccgtcgcc ggtcacgctc
    66121 ttccgggaag tccgccgcct gccccccgca agccgcctcg actggcagaa cggcagcacc
    66181 cgtcaggccc cctactgggt gccggtcttc cgggaggaca cgcgtgtgtc cgtggaggac
    66241 gccaaggccc gcttcatgga gatcgtgcgc gactccgtca cccgcgagat cgaccgggac
    66301 gacgtgggcg catttctctc cggcggcacc gacagttcca ccgtctcggg catgctcggg
    66361 cgcatcaccg gccggccggc gcggacctac tcgatgggct tcgacgccac cggctacgac
    66421 gagatggagt acgcgcgcat cgccgccaag cacttcaaga ccgatcacca cgagtactac
    66481 gtcaccccgg aagatctcct cgaaggcatt cccaaggtgg cacgcagcta cgaccaaccc
    66541 ttcggcaact cctcggccgt gcccgcctgg atctgcgcca gccgcgcccg cgccgacggc
    66601 atcggcaagc tgctggccgg cgacggcggt gacgagctct tcggcggcaa cgcccgctac
    66661 gccaaacagc gcgtcttcgg ctggtacgac accatcccct cgcccctgcg cagcggcctg
    66721 atcgaacccc tcagcggcct gcccggcatg gaccgcatcc ccctcatcaa gaagggcgtc
    66781 agctacgtgg agcaggccag cgtgcccatg cccgaccgca tgcagatgta caacctgctc
    66841 ctgcgcctgg gcctcgacac cgtgttccag cccaacttcc tcaagggcat cgacaccggc
    66901 gagccgtccc gcctgcagcg tgacacctgg gccctggtgc cggaaggcag cctgctcaac
    66961 cgcatgctcg ccttcgactg gaaatacacc ctcgccgaca acgacctgcc caaggtcatc
    67021 ggcaccaccc gcctggccgg cgtggacgtg ggcttcccgc tgctgtcgga cgagctcctc
    67081 gacttttccc tgaccctgcc cagcgagtgg aagctcaagg gcttcaagct gcgctggctg
    67141 ttcaaggagg ccctgcgcgg cttcctgccg gacgagatca tcaccaagaa gaagcacggc
    67201 ttcggcctgc ccttcggcgt atgggccgtc aagcaccccg gcctcaaggc cctcgcctgc
    67261 gacaccctgg aagccttcgg ccagcgcggg gtgatgagcc ccaccttcct ccacgacctg
    67321 cacgccaagt ggctgcccga acatccgggc tactacggtg agctggtgtg gatcgtcatg
    67381 atgctcgaac agtggctgca ggcctacgcg cccgactggc gcctcggcgg cccggcccgg
    67441 gcatgacggc cgcccgcccc gacgcaccgt ctgcagcctc tgcaagccgc ccacccgccc
    67501 gtgcgggcgc ggcgctgctg cggctgggcc tgccgctgct cctcatcgcc ctggtgtggc
    67561 tggctcacta tgcccatttc cacgtggacc gcagtccggt ggcagaagcc gtggtcgagc
    67621 aggcccggca ggcgcccgac gacgcccgcc tcgacgagat cggccacatg tccctggcca
    67681 tcgacctggg cgtcccgtcg gaccgggtcg tggccaccgc cgatctgatc gtgcggggca
    67741 ccgccctctt tcccgcctac ctggatggcc cgacgccgct gaccggatat ccggcggact
    67801 tccgcttcgg tcccggtacc ttccagctcg ccgccgccgg attcggcttt gaagacatcc
    67861 tgctggaagc ctacgaacgc accgccgacg cccgctacct gcgcaccgcc taccagcgta
    67921 ttcaggattt cgccgccttc gagctcaccc agcgggaagc cgtcgatttc ctgtggaacg
    67981 accacgccat cgcctgccgc gttgccgtgg tggcgcgcct gtggcgccac atccgccacg
    68041 acccggccta ccccgccgac ggtctgcgca acctgctgtc cctcatcgcc cgtggcggtc
    68101 gctttctcgc cgcgccggac cacttcaccg cacgcaccaa ccacggggtg atccagaacg
    68161 tcgccctcat gcagatcgcc gcggccttcc cgagcctgcc cgacagcgcc ggctgggccc
    68221 gcctgggcat ggagcggctg gccctgcaac tgcccttcta cgtatcgccc gaaggtgtcg
    68281 tgctggaaca ctcggcggat tatcagcacg tgggcgtcag cctgctcgcc atggccgtcc
    68341 gcctcgcccg cctcaacgac ctgccggttt cccgcgagct gctcgacaag tctgccgcca
    68401 gccggaccgt ccagagccag ctccagcgcc ccgacggcag tctgcccgcc gtcggcgact
    68461 ccatcgtggg acgcccccac accgccgtca ccggcgccac cgacggctcc acacccgtcg
    68521 ccgaacagcc ggccccgttc ccgctccctg agcccgctgc ccacgtctat ccggtctccg
    68581 gctacgccct gtggtggacc ggagacgatc cagccctgcg cagccagacc gtcatcgcct
    68641 gggccaaaca cgacggccac ggtcacaagc acgccgacga aggctcgctg ctcctgtggt
    68701 ccggcggccg tgactggctc accagcatcg cctactggcc ctacggtggt gccaacgaaa
    68761 aggctgccta cggctggtcc ggcgccaatg ccccccacgg tatcggtgag cttgccgcca
    68821 gcgtgcgcac cgtggaactc cttggcagcg ccgacgacgc ccagatgcac gccgtggacc
    68881 tgctccgccg caccggcgac ggcgcccaat tccggcgcca gatcgtccag gtcgacgccc
    68941 agacctggct cgtgctggat ttcgttgacg gaagcgcatc gggcagcgaa accgactgga
    69001 ccctgccgcc ggcattgggc ctcaccgccc gtaccgacga ccgcagcttc cagtccacag
    69061 aagccggggg gcgccggctg atcctcagcc tggcggcgcc ctccacgacg cgcagccgca
    69121 tcctgcgagg ctcccaggac cccttcgcag gctgggtgtc ggtgaatctg cgcccaacgc
    69181 ccgccagcac gctccgggtg accgacccgt cggcccgctc ggtgaccgcc ctcctgctcg
    69241 ccctgcggcc cgcagaccgg atcggcccac agctcagtgt ggcgcccggt gcgacgcccg
    69301 aagcctggtc gatccacatc gacgaggaag gctcccgcca tgcggtgacc cgtcagggcg
    69361 cccgcctcgc gatggacgat gccagcgtgc tcagccttgc cgcacctgcg gacattgcac
    69421 cggcgcaagc cgtcctgcgc gccgcttatc gcaaggcggt ggacgccttc ccgccctggc
    69481 gcgacctgtc acgcttccgg gcacggatcg catacggatt gctcgcattg atggtggccc
    69541 aggagatcgc atggcaggtc ttcatccgcc ggccggccac cacgcccggc cagcgtcgcg
    69601 gggtgctgct aggcctgagc gcgggatgga tcgcgctggg tgcgtggatc ttcgggatct
    69661 atctggcctg agccccgacc accggatgtt caccgttccg gtacgcgcag ggaccgggtt
    69721 gccgtcagaa tccatcaccg ccggtacacc ggacggcaac ggccgccttt tgtagggacg
    69781 cacgatcgac cagacggctg ggcccccaga cgccggcacg cccggtcccc cacgcacgcc
    69841 cgcgctgcac cgcagatcag cggaccggcg cacccgcttc gctgctccgc cccatcgggc
    69901 cgatgtagga cgtggcgacc accacgttgt cgatgtacag gctcatgtcc cgcggcgacg
    69961 gatcaacacc gccgtaatag acgttgaacc agacgctctc gatccgcagc tggggaacat
    70021 tgcgcagctg gatgtcggtc cgctccacca ccagccggcc gtcgatccac gcccgcagga
    70081 tgccgtcgga cttgcccggg gtgttgagct tcaggtactg ctccacgcag taccagcgat
    70141 tgttcgccag ggctgccagg cgcccgaggg gccagggcca gaagtcgccg gagggatcgc
    70201 gcatggcggc gtgataggcg taagtgccca tgccggtgcg cccggacagc ggattgccgc
    70261 ttcgggacac catggagaac tgcccgcgca gggaccagcc atcgtcgccg gtactcttgc
    70321 gcagccccca ccctgccttg ccgtaggtgc cggcgaatcc cggcatcttg ccggcgctct
    70381 gctccggccc ccagtcggcg gcaaagcgca ggtagtagcg gaaataggcc tcttcaggct
    70441 ccgaaccggt cttgtcccgg tagttatagc gcagatccag cccgagattg ccgttgcgcg
    70501 ggaccgtcac ccgcaaggcg cggccgctga ggggcaggaa cagccgctcg ggatccgagg
    70561 tgacggtggt ggatgtgctc cgcgaatcat attccgacca cgccgaactc cagcgatccg
    70621 cctcgaaatc cgaaaagaac agcactctgg gatccttggc gatgcccgcg tcccccacat
    70681 aagccgccgc cagacccggc cgcggcagac gatccggaga ttccggcgcc cgcaccgcaa
    70741 acacacccag cgtcgtgtcg ttgccgtact gatcgtccac acgcattgtc aggaccgcct
    70801 tgtcgaggac acgcccggca aatttctcga ccggcagggc gaaggccagc aggctacgat
    70861 aggtgccgcc ggcgcgcaga cgcggttcga atccgaggcc cttttcggtg gagcagtcga
    70921 gataggcatc cgacaccgga tcgaaggccg tgcgacggcc gtccttgaat tccaggcgca
    70981 ggagcggtcg ggccgtgatg tcgggatgct cccggctggc aaagacgacg atgttgccgc
    71041 cgggaccgct ggtggacatg agcatcgcgc cctgggccgg atcggccttg ccggccagcc
    71101 agtcgcgggc cagtccggtc atgtcgatct ccaccagatg cccgcgactg ccttcgcgga
    71161 tcaccgtcgt ggaataggcc cgcgtaccgt aggaacgccg gtccgcatcg gcccagtcac
    71221 ccccctcacg gttccagcgc ccacctccga cgccgttgaa atagtggcag ctggcgccgt
    71281 cgatggcgtc gggcaggctg ttttccggcg atgcggccca cgcgccaagg gtcaggacac
    71341 ttgccccgac ggccagcatc cgcccgagca ggcggatcac tcgccggccc tcgtcgcggc
    71401 cgcgccgggc gcagcggcgg cggcaccgga ggc
//
LOCUS       DAOQPA010000006        66227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_146_length_66227_cov_18.315768, whole genome shotgun sequence.
ACCESSION   DAOQPA010000006 DAOQPA010000000
VERSION     DAOQPA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 66227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66227
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_66227_cov_18.315768"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="ccsA"
                     /locus_tag="PKX01_01650"
     CDS             complement(<1..781)
                     /gene="ccsA"
                     /locus_tag="PKX01_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ74851.1"
                     /translation="MIPELGSFALVLALLVALVQGVLPLVGAQRGIPAWIALARPASA
                     TLFLLVAVAFSCLTASFINNDFSVLYVVQHSNSRLPVEYRIAGVWGGHEGSLLLWVQM
                     LALWSLAVAAFSRSLPEDMVARVQAVLGLVAAGFILFILTTSSPFERLLPGAEDGRDL
                     NPLLQDPGLIFHPPMLYMGYVGFSVAFAFALAALLSGRLDAAWARWSRPWTTAAWIFL
                     TLGIGLGSIWAYYELGWGGWWFWDPVENASFMPWLVGTALIH"
     gene            complement(798..1238)
                     /gene="ccmE"
                     /locus_tag="PKX01_01655"
     CDS             complement(798..1238)
                     /gene="ccmE"
                     /locus_tag="PKX01_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMZ74852.1"
                     /translation="MKARHKRFAIVLGGLAALGIAATLVLNAFQSNLVFFFTPTQVAA
                     GEAPKDRAFRIGGLVKEGSLQRTDITARFTVTDTAKEIPVTYTGILPDLFSEGKGVVA
                     QGRLGVDGQFTASEVLAKHDENYMPPEAKAAVDQAHQAAKSLKQ"
     gene            complement(1235..1423)
                     /gene="ccmD"
                     /locus_tag="PKX01_01660"
     CDS             complement(1235..1423)
                     /gene="ccmD"
                     /locus_tag="PKX01_01660"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HMZ74853.1"
                     /translation="MNWGSASEFFAMGGYGLYVWGSFGVTFGALLIEALLTRARFRAA
                     RRALRRELAADRESQDHP"
     gene            complement(1420..2169)
                     /gene="ccmC"
                     /locus_tag="PKX01_01665"
     CDS             complement(1420..2169)
                     /gene="ccmC"
                     /locus_tag="PKX01_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HMZ74854.1"
                     /translation="MSQRLINWFKFASPASFYPLAGRAWPWFAALAAAFAAAGLWLGF
                     FVAPTDAQQGEGYRIIFIHVAASWMSMFIYCVMAFWSALGISLNTRLSGMMCSALAPT
                     GALFAFLSLWTGALWGKPMWGAWWVWDARLTSELILFFLYLGYIALTSAIDDPRRADK
                     AGAIISLVGVINVPIIYFSVKWWNTLHQGASVSLTKAPSMATTMLWGMLLMALAFWMY
                     SIATALYRVRAIILERERHAAWVAELPEMQA"
     gene            complement(2191..2856)
                     /gene="ccmB"
                     /locus_tag="PKX01_01670"
     CDS             complement(2191..2856)
                     /gene="ccmB"
                     /locus_tag="PKX01_01670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMZ74855.1"
                     /translation="MNAFIAIVRRDLLLAARRKSEVLTALFFFLIVVSLFPLGIGPET
                     AMLRRIAPGVLWVAALLAAMLSLHRLFAADHQDGTLEQMALSPVPLALLVGGKIVAHW
                     LTSGLPLVLLAPVLGIQFDLAPQALGILMLSLLLGTPVLSLIGAIGAALTLGVRGGGV
                     LLSLLVLPLYIPALIFGAGAVEAEMAGLGAGGHLSLLGAVLALGAFFAPWAATAALRI
                     ALE"
     gene            complement(2853..3467)
                     /gene="ccmA"
                     /locus_tag="PKX01_01675"
     CDS             complement(2853..3467)
                     /gene="ccmA"
                     /locus_tag="PKX01_01675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HMZ74856.1"
                     /translation="MLRAHGLACDRGDRRLFSGVDCAVAPGEWLQVEGPNGAGKTSLL
                     RILAGLAPAAAGHIEWEGRPVADVRDEYHARLLYLGHAPSIKDDLSALENLTIAAAVA
                     GRPLDEAAALGALRRIGLKGREDLPSRVLSQGQKRRVALARLLCSQARLWILDEPFVA
                     LDVAAVDQLCAILDDHLGRGGMLLFTSHQAVQLSGAGGSLRLGA"
     gene            complement(3655..4353)
                     /locus_tag="PKX01_01680"
     CDS             complement(3655..4353)
                     /locus_tag="PKX01_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74857.1"
                     /translation="MSTIICIIGNKGGTGKTTLSHMLCQGFGLLGQRSACVLTDVSRE
                     PLSPDGRRYVTADGRSPEALHKVVGKLRTMQDWIGVIDGGGNRSEVDRLLDSYADIVL
                     LPFRDSHEDMRTVRRDLETFPRAYGLPSQWATNAWQRDAADRAVLELLGDHRDRLLHP
                     VASLSASKLLLQKHIPASLPSNLGNACRALARQVMDVLETDADALGTDAHEAAHSLPA
                     PTVLPAGYASTAHH"
     gene            complement(4468..5265)
                     /locus_tag="PKX01_01685"
     CDS             complement(4468..5265)
                     /locus_tag="PKX01_01685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ74858.1"
                     /translation="MRLPPALKALLAQLAGWAVAIGLLRAGLLPGQAWALVLAQAIAA
                     VSAAVALRSAPWWRWIHAGFTPLAALALQLGLHPAWYLAAFALLALVYWSSFRTQVPL
                     FLSNPATAAAVAQLVPPGHSARVLDLGSGTGALLRPLARLRPDCRLEGMESAPAPYLL
                     SRLLSRRHPNIALARGDFWQIPWTDYDLIYAFLSPVPMPEVWRKACAELRPGALLVSN
                     SFPVEGIEPDDIIHVADRRGTRLYCYHPAGPLPPDGRAVGSPVGLLP"
     gene            complement(5333..7063)
                     /locus_tag="PKX01_01690"
     CDS             complement(5333..7063)
                     /locus_tag="PKX01_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ74859.1"
                     /translation="MPTSLTVTVGQYSDKGRKPANQDFHGLCIPVEPQLTTKGIAAAV
                     ADGISSSEVSHLASESAVKSFLGDYYCTAETWSVRTSAQRVLEATNAWLHAQTRNSPH
                     RYDKDRGYICTFSGLVLKSHTAHLFHVGDSRIARLRDGRLEVLTDAHRIVVSPEQSYL
                     ARALGFEAQLELDYRQVPITAGDVFVLSTDGVHEHVGADVIGATLRAAPDLDAAARRI
                     ADAALAQGSADNLTIQLIRVDTLPVQAAAEHLQQFAELPLPPLLDARMEFDGYRIRRT
                     LHGSSRSHLYLAEDIASGTRVALKLPSIDQREDKAYRERFMLEEWIARRLDSPHVLKP
                     CPVTRQRSYLYVATEYIEGCTLAQWMRDHPAPDVETVRGIVEQIARGLLAFHRLEMLH
                     QDLRPENIMIDTQGVVRIIDFGATHVNGLQETAANDTLAPILGTCQYTAPEYFLGEPG
                     TSRSDLFSLGVIAYQMLCSRLPYGTDVSQARTRSAQKKLRYRSVLSDTREIPAWLDAA
                     LAKAVHPDPLRRYAELSEFTYDLRHPNAALLGQRPPPLIERNPLLFWQILCALQTVLL
                     ILLLARRSLL"
     gene            complement(7117..7926)
                     /locus_tag="PKX01_01695"
     CDS             complement(7117..7926)
                     /locus_tag="PKX01_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HMZ74860.1"
                     /translation="MSYLAPTEFVTKMVDAGESKIFMSTRDTLIRAYMAGAILALAAA
                     FAVTINVQTGQPLAGAILFPVGFCMLYLLGFDLLTGVFTLAPLALLDKRPGVTVGGLL
                     RNWGLVFVGNFAGALTVAVMMAIIFTFGFTEAPNAIGQKIGTIGESRTVGYAAHGAAG
                     MLTLFIRGVLCNWMVSTGVVAAMMSTHVTGKIFAMWMPIMVFFYMGFEHSIVNMFLFP
                     SGLMLGGNFSLYDYMVWNEIPTVVGNLVGGITFVGLTLYSTHVRTAPKRNA"
     gene            complement(8272..8655)
                     /locus_tag="PKX01_01700"
     CDS             complement(8272..8655)
                     /locus_tag="PKX01_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74861.1"
                     /translation="MLEPITDHKGLHIVSIGGRDAQARRYFVQDGEQLISKALPSLDT
                     ARAEAERLLAKRARGPNPWRAPWATHLIAPGVAVIQYPLGRALLLALSGGHYPPRGPH
                     NSLFVVSFPWMLLYAVFGVMSVFID"
     gene            complement(8736..12308)
                     /locus_tag="PKX01_01705"
     CDS             complement(8736..12308)
                     /locus_tag="PKX01_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HMZ74862.1"
                     /translation="MAEQDPVRARPPGATLDDKYTAATGRVYLTGYQALVRLLLIQRD
                     RDRAAGLRTAGFVSGYRGSPLGGLDQTLWKAQAHLAGHDIVFQPGLNEDMAATAVWGS
                     QQVSLSPGARVEGVFAMWYGKGPGVDRCGDVFRHANAAGSARHGGVLVIAGDDHAAKS
                     STLPHQTDHFFKSMMMPVLAPAGVQEYLDFGVHGWAMSRYSGCWVAFKALADTVETSA
                     SVDVDPLRVVTRLPEDFALPPDGLNLRWPDPPLVQERRLLHYKLYAALAYCRANDLNR
                     VVIDSPEPRLGILTSGKSYLDVRQAFDDLGIDETLAARIGLRLYKVGMVWPLEAEGVR
                     RFAEGLDEILVIEEKRQLLEYQLKEELYNWREDVRPRVVGKFDEKGEWALLTTVGPDG
                     RATVDHGDWLLPAAGELTPAMIARVIAARIGRFFTSEHIQARLAFLDAKEHALAHRVF
                     ALDRVPTFCPGCPHNTSTRLPEGSRGLAGIGCHYMVSFMPDRRTGTFTHMGGEGVPWV
                     GQAPFTDERHIFANLGDGTYFHSGLLAIRQSVAAFNRPGATHGITYKILYNDAVAMTG
                     GQPVDGVLTVPALLRQLQAEGVHTIALVTDGTARSWHVADIPHGVTIHHRDELDVLQR
                     QLRSVPGVSALVFDQTCAAEKRRRRKRGTYPDPQTRVFINEAVCEGCGDCGRASNCMA
                     ILPVETEFGRKRRIDQSACNKDFSCMDGFCPSFVTVEGGALRRGSARHTTDARFPALP
                     APVLPPTGRPWGILVTGVGGTGVVTIGALIGMAAHLDGKGVTVLDMTGLAQKGGSVFS
                     HIRIADRPEALHAVRIAAGEADAVIGGDVVVTASVDALAKMAVGRSHGVVNCAETPTS
                     DFTRNPDWQFPLERMQAALVEAVGADACDFLDATDLATRLLGDAIATNLFLLGYAWQK
                     GWVPVSEASLDRAIELNGAAVALNRAAFLWGRRSAVDRAAVEALARPAATPQDDTQTD
                     GLDARIARRAAFLTDYQDAAYARRYTDFVARVRTAEAPLGSERLGEAVADGYFRLLAC
                     KDEYEVARLFTAPAFRAAVDAAFEGDYRLRFHLAPTFLARPDPATGRLRKLAFGPWLM
                     TAFRGLAWLRRLRGTPFDLFAYHPERKAERALTAHYEADIADLLSDLSATTLHVAEAV
                     ARLPETIRGYGHVKADAMAQAAQRRTALLAELAALRALRRPSGT"
     gene            12622..12954
                     /locus_tag="PKX01_01710"
     CDS             12622..12954
                     /locus_tag="PKX01_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74863.1"
                     /translation="MKSLIPVSCLILLCGFAQAQASEAHTLRATPADRADTCAHGGER
                     HGLEYAHWNEYLATRAGDSQSARDWAERARAECRELASERRDGPERPEARPDVRQERA
                     RRRELARR"
     gene            12967..14583
                     /locus_tag="PKX01_01715"
     CDS             12967..14583
                     /locus_tag="PKX01_01715"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMZ74864.1"
                     /translation="MSRRRIPATPEPARGPSPRQILNRLGFGIGPSDELARLARDGLP
                     AWLDAQLAPPPGDDGECLQRLGAARLRIRYDAGKAPGPEYPAVDENRPLETLQAPIET
                     LWPRTDYKRPMAGSERFRPRAEVAAATLIRAVHSRWSLREVLADFWHNHFNVDASQIV
                     QVAVALPAYDRDVIRAHALGNFREFLEAVATSTAMLYYLNNRSSRAGAANENYARELF
                     ELHTFGRGAYLNEFYNRWRDVPGALTGQPKGFIDQDVYEAARAFTGWTVEDGSGLGGS
                     DRLPATGRFAYVDSWHDNYQKRVLATEFDPFRPALEDGRKVLDLVAAHPATARFVSGK
                     LCRRLLGVEPSAGMLAAASETWRAALRAPDQIARVVRTIVLSPEFARSADLRVRRPLD
                     LVAALVRGAGMDFVPTEGLVNEMAAAGQRLFGWGAPTGHPEEVDYWLSSNGMRRRWLL
                     VQGLIENRWGNGVVEVRPPTPASTWREAADAWAEHLLGEAPGSTPVGQILAGLRQDGA
                     TPFDPGKPAQAAQMRRVLGYLAMGPGFQRG"
     gene            14588..15844
                     /locus_tag="PKX01_01720"
     CDS             14588..15844
                     /locus_tag="PKX01_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ74865.1"
                     /translation="MTTRRSFLKACAAAGALMPGLGIGRLAYAATDAAAPLTVVVFLR
                     GGCDGLLLAGPANEPAYVEARPPELRVLDGGERAGLPLANGLTADLDFRLHPDARPLY
                     ELYQAGRLALVHAVGLTNGTRSHFEAQDLIERGVSTLEQAYRTPEGWLTRYLTLYGGA
                     DGSADHLAALAAGAGLPASLARQGDALALPDLQGGVGLPGGTDGAALLGQLYAAGDSP
                     VHEAGRRALARLSQVDAHLPRTPDGKLAPYAPDGAGYESGAFSLGLQAIARVARMNVG
                     LRVACIDHASWDTHDAESGRFGGLVGQLSRGLAAFHRDLASAGIPFRLVAMSEFGRRL
                     RANRSQGTDHGHGGAMLVLGEGVRGGRMLGRWPGLSTPALDQGVDLAVTTDWRAVLAE
                     LLALDGRGRGLVFPGLPVEAPLGVLG"
     gene            complement(15871..17100)
                     /locus_tag="PKX01_01725"
     CDS             complement(15871..17100)
                     /locus_tag="PKX01_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ74866.1"
                     /translation="MIRTRFRADDFAILVALATSAGGIASAALGGPAGLSLVAGTFTV
                     VALVTAMIVSRRARERWRQQQQAQRESIDSSVREYDMLCDQIAVQSGFQYERLRESLG
                     QMQDVVSSAASKLGGSLAGGQGGNSRDQLRHLVDELLSLAAEEERALQESGLERFAAE
                     TRDALRDFVGTVERLKASGSDIAERFGAMRGKVDAVTRLMTEVNQINSQTELLALNAA
                     IEAARAGEAGRGFAVVADEVRKLAQRTETFSDQIATLLTDIHASIDDVGRAVDVTAAT
                     DVSKARSSEASVSAMGLEMSELNARAMVQSRRISELSAKIHRLVMEGIQSMQFEDIVR
                     QILDKLRQHTDFMGRYAHGFFDAHRDPEQREATSRIQRRVEILRQLLAQSEQSKKDIR
                     FEAVQQTAVHAGEVDLF"
     gene            17417..17797
                     /locus_tag="PKX01_01730"
     CDS             17417..17797
                     /locus_tag="PKX01_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ74867.1"
                     /translation="MPKQIIATPHAPAAIGTYSQAVRAGDTVYLSGQIGLDPATMQLM
                     DGFEAQTVRVFENLKAVAEAAGASLADVVKLNLYLTDLAHFAKVNEVMARYFSEPFPA
                     RAAVGVKELPRGALVEADAVLVLG"
     gene            17805..19871
                     /gene="recG"
                     /locus_tag="PKX01_01735"
     CDS             17805..19871
                     /gene="recG"
                     /locus_tag="PKX01_01735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMZ74868.1"
                     /translation="MSAPPPETGPGPRGWPGVGPQLAGRLARLDIFGPQDLILHLPLR
                     YEDETRVTMLADVRPGCAAQCEVTVLHCEVQLRPRRQLVARAADASGEVSLRFVHFYP
                     SQQKQLAPGARLRVFGEVRPGFFGAEMIHPRVRPVAEGESLPESLTPVYPTTAGLAQS
                     ALRKLIGGAMRHHPPREDLPDEVVRRLDLMALPDALHLLHAPPPSLAAVELEDRHHPA
                     WQRIKLDELLAQQISLRRAYAARRAKNAPPLPAVDTLTRALLTELPFSLTGAQRRAWD
                     EIARDLAAAHPMQRLLQGDVGSGKTCVAALAMLRAAENGRQAVLMAPTEILAEQHYRK
                     LAGWLAPLGLEVAWLSGSRRKREREALIARLAAGELLLAVGTHALIEDPVSLPGLALA
                     VVDEQHRFGVRQRLALREKGRGGAPHMLMMSATPIPRTLAMSYYADLDVSVVDELPPG
                     RTPIVTKLVAEARRDEVIRRVHDACVAGRQAYWVCPLIEESEALQLKTAEESFALLAA
                     ALPDLKVGLVHGRLRNDEKSATMAAFVAGEIHVLVATTVIEVGVDVPNASLMVIEHAE
                     RFGLAQLHQLRGRVGRGAHASACILIYAQPLSQTGRERLKAIYEHADGFVIAREDLRI
                     RGPGEFIGARQSGVPLLRHADLEADADLVAVARDLAETLLRDAPAVADAITARWFGGR
                     AALLKA"
     gene            19969..20538
                     /locus_tag="PKX01_01740"
     CDS             19969..20538
                     /locus_tag="PKX01_01740"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HMZ74869.1"
                     /translation="MLRRSMTLAHFPGRDPFRMTASRAPRPLRGWLSDDSSLTARIRA
                     RCGTFSLHLVSQGKGRPLPDELAALGVHRHGELWQREVLLLADGAPVVFARSLVAAST
                     VPAAWHLLRHLGGRPLAAVLFDDPRVRRSPLEAARLDPRDTRWHRAAAATGRSDLPAL
                     WARRSAFRRAGSPLLITEVFLPAIRSLAP"
     gene            20535..21407
                     /gene="ubiA"
                     /locus_tag="PKX01_01745"
     CDS             20535..21407
                     /gene="ubiA"
                     /locus_tag="PKX01_01745"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HMZ74870.1"
                     /translation="MTFAERLSEYEKLMRLDKPIGILLLLWPTLWALWVAGGGRPSPF
                     IVWIFVLGTVLMRSAGCVINDYADRNFDGHVERTRNRPLAAGRVRPGEALVLAAVLAL
                     VSFLLVLPLNTLTLLLSVPAVFLAGSYPFTKRFLAIPQAYLGIAFGFGIPMAFAACLD
                     AVPVEAWVMLLANVFWAVAYDTEYAMVDRPDDLKIGIKTSAITFGRFDVAAVMLCYAV
                     TLGLLAWVGLHTGRGAAFLAGLAVAAGLMGYHFTLIRGRDRANCFRAFLHNNWVGGAI
                     FAGWALDYLLKPVG"
     gene            complement(21450..22730)
                     /gene="waaA"
                     /locus_tag="PKX01_01750"
     CDS             complement(21450..22730)
                     /gene="waaA"
                     /locus_tag="PKX01_01750"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HMZ74871.1"
                     /translation="MILRFLYTLLWLLAAPFIVLRLVWRARRQPEYLQHLGERLGRYP
                     QRLSLPVIWIHAVSVGETRASAPLVQALLDAYPDHSLLLTHMTPTGRATGEELFGRFA
                     PRVQSAYLPYDLPWVMRRFLRHFRPSMGILMETEVWPNLVETCRRRHIPLALVNARMS
                     ERSARGYRRAGPLAREAFAHLSVIAAQTESDENRMSALGARYLKVTGNIKFDIMPPTT
                     MQALGHEFRNRFGPRQVLLAASTREGEEELLLETFARLCPPEVLLVLVPRHPQRFGEV
                     VKLVRKAGLGLQRRSSNDPVLADTRVWLGDSMGEMFAYYHASDVALIGGSWLPLGGQN
                     LIEACAVGCPVVVGPHTFNFSAAAEQAIEAGAAVRASDMDDGIGVALQILQDPARRSA
                     MGAAGQAYAQTHRGATERTLDLITPLLRAARPRR"
     gene            22907..23914
                     /locus_tag="PKX01_01755"
     CDS             22907..23914
                     /locus_tag="PKX01_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HMZ74872.1"
                     /translation="MKILVTGAAGFIGMHTSQLLLARGDQVVGLDNLNDYYEVALKHN
                     RLARLTPHENFRFVKMDVADRDGMAKLFAEEKFDRVIHLAAQAGVRYSLINPHAYVDS
                     NLVGFMNILEGCRHSQVEHLVYASSSSVYGGNTRMPFSEHDSVDHPVSMYAATKKANE
                     LMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVFNHGKMKRDF
                     TYVDDIVQGVVRTLDRVAEPNPAYDADTPDPGTSNSPFRVFNIGNNNPVELMSFVEAI
                     EKALGKTAEKNFLPLQDGDVPATYANTDALNAWTGFVPATPVDVGIGRFVEWYRSYYK
                     V"
     gene            23973..24761
                     /locus_tag="PKX01_01760"
     CDS             23973..24761
                     /locus_tag="PKX01_01760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ74873.1"
                     /translation="MDRLTVSIVLYRPDARLLQATLDALGEACREAALTPELFLIDNG
                     GGAAIMASLRWPADWTAHVLTGHGNVGFGCGHNLSLAGAGDIHLILNPDLEMAPDALR
                     AARDFLRGHPECGLVVPAARWDDGRIQYLCKRRPAVFDLFLRGFAPAWLRRRFAARLA
                     RYEMQDVIGDAVVWDPPLVSGACMFFRTDVLRRVGGFDPRFFLYFEDFDLSLRVARIT
                     RIAYVPAVRVVHHGGHAARKGWHHVRLFMQSAARFYGLHGWTWR"
     gene            24758..25621
                     /locus_tag="PKX01_01765"
     CDS             24758..25621
                     /locus_tag="PKX01_01765"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HMZ74874.1"
                     /translation="MNDLDQRRRRGLKAGLALLAGVMLGRAQAATPPPLLLAEVLHGD
                     VDVTRYWVSEKLDGARAIWDGQTLRFRSGREVPAPAWFIAGLPPEPLDGELWMGRGTF
                     DALAAAVRRQPARDDEWRRVRLMVFELPGGAGSFTDRAARIREIIARADVPWLQAVAQ
                     FRVPDRPALQARLDAVVRAGGEGLMLHRDDAPYLTGRHDALLKLKPQLDAEATVIAHL
                     PGKGRFSGRLGALEVETPDGVRFRLGSGFSDAVRRDPPALGSRVTYRYRSLTPGGVPR
                     FASFLRLRVEF"
     gene            25618..26580
                     /locus_tag="PKX01_01770"
     CDS             25618..26580
                     /locus_tag="PKX01_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ74875.1"
                     /translation="MSGRILVTGGRGFIGRRLVTALQADGACPRVLTRQTPAAPSEVQ
                     GDVTDVTALARACEGVETVFHCAGHAHAFKTHAADEIARHWAVNFEGSVRLIDAAAAA
                     GVRRLVFLSSVKAVGEPGDAQADETWSAAPEGAYGAAKRAAEAALLEAGGRHGLHVVI
                     LRPVMVYGAGGRGNLERMARLVRRGCFPPLPETGNRRSLIHVDDVVSALRVVASDPRA
                     AGRTYILAHPIACSGRALYDELRRVMGLAPIAWAVPARLLQAAAWLADGCGGLAGLHL
                     PFNGEVLDRLLGSACYSSERIRMELGWQARVVLADGLKEMLGGR"
     gene            26591..27568
                     /locus_tag="PKX01_01775"
     CDS             26591..27568
                     /locus_tag="PKX01_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ74876.1"
                     /translation="MLAVGLAFGAAWGVTRTCCRPDSLLHFVDQPNERSLHVRPTPRT
                     GGVGVMAGVLLGGGLALLAGHGSMGLVLALAGAVLLAVVGLADDRLDLSARVRLLVHL
                     GVAAGFVVISDAGGGVEAWRVLPLVLGLVWMTNLYNFMDGSDGLAGGMAVWGFVAYAV
                     AAGGHGDGTLAAVCASIAAAAAGFLCFNFPPARIFMGDSGSVPLGFLAGALGCVGVMG
                     NRWPAWFPVLVFSPFIVDASVTLARRALRGERVWQAHRTHYYQRLVQMGYGHRGVAVR
                     AYGLMAACGGSALLVMDRGVALQCFVITMWTFVYMVVGFRIDRAWRRSG"
     gene            27579..29462
                     /locus_tag="PKX01_01780"
     CDS             27579..29462
                     /locus_tag="PKX01_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HMZ74877.1"
                     /translation="MRSLQKFAHPLLVFLSDALGICLAWFVAYLIRFNLAIPAEFMPA
                     MLFGLAVALPIQCLFLRVFGLYRGIWVFASLPDLHRIGRAVAAGAVVVPLVLVVSVRT
                     EPWIPRLVFLLQPVLLLLYMGGTRALYRSWKEFRNFGALRAQGQSVLILGAGVAGAHL
                     VRELAASAQWRVVGLLDDDPHKQRREIHGYKVLGPISELERWAETLKVGHAIIAMPSV
                     RHEVRRAVASECVRVGVTALIVPGVDERLSTSHSLSEVRQLDVEDLLGRDPVRIDTEQ
                     VQDYLRGKAVMVTGAGGSIGAELCRQIARFEPSVLVCFELSEFALYRLIEDFAREFPR
                     LQLVALAGDVKDAVRVDEVIGRYRPNVIFHAAAYKHVPLMEEDNAWQAVRNNVMGTFQ
                     VARAAVAFGVPRFVLVSTDKAVNPTNVMGATKRLAEQVCQAFALEGRSQFEIVRFGNV
                     LASAGSVIPKFQAQIAAGGPVTVTHPEITRYFMSIHEAAQLVLQAGSMGQGGEIFVMD
                     MGEPVRIQDLARDMVRLSGRSEDDIRIVFTGLRPGEKLYEEVLADSERTRPTHHPKLR
                     IARAQAVESGWLDMLALWLRQRRAVPAAEVRRELRRWVPEYTPSSHAVPLTKVSDTGR
                     QRA"
     gene            29472..30305
                     /locus_tag="PKX01_01785"
     CDS             29472..30305
                     /locus_tag="PKX01_01785"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HMZ74878.1"
                     /translation="MATYAIGDIQGCFSALQALLKRVEFDAGHDRLWLVGDLVNRGPD
                     SLKTLRFVRDLGPAAVTVLGNHDLYLLMVAYGTVRSRGKDDTIQAVLDAPDREALLGW
                     LRTRPLMHVENGFAMVHAGLLPGWTVSQARALAREVEGALAGPYHADLLHNMWGSEPA
                     AWRDDLRDYERMRVIVNAMTRMRFCTPEGVMDFKVKGEVARAPKGYLPWFDAPARRST
                     DATVVFGHWSALGLKIRPNLMALDSGCLWGRELTAVRLEDRQVFQVSCPGLSHGKPQP
                     S"
     gene            complement(30307..30888)
                     /locus_tag="PKX01_01790"
     CDS             complement(30307..30888)
                     /locus_tag="PKX01_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ74879.1"
                     /translation="MRLDVGGLPVAPGGVLIANHVSWLDVFVINATCPSTFISKAEVR
                     AWPVIGWFAARNETVFLRRGSRGHARIVNGEIATLLARGRHVTIFPEGTTTDGRGVLG
                     FHGALLQPAIQVGAPLQPVALTYRDATGRYTRAPAYDGDLSLVDTLLNIVAERTIVAR
                     VRLLAPLSTGETPDRKQLARSAREAIVSCIAID"
     gene            complement(31050..31808)
                     /locus_tag="PKX01_01795"
     CDS             complement(31050..31808)
                     /locus_tag="PKX01_01795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HMZ74880.1"
                     /translation="MLHKEQHVAHRPGRNLQVTLAASQTEVLEAQKLRYRVFADEMGA
                     RLQTRIPGVDRDLFDAFCDHLIVRDEDAGRIVGTYRILSPSAAREVGSYYSESEFDLT
                     RLRHLRERMVEVGRSCIDPDYRSGAVITLLWAGLARYMVENGHDYLIGCASISMADGG
                     HAAASLYNRLKDSHLCPLEYRVFPRTPLPIGALRQDLDAETPPLIKGYLRAGAWICGE
                     PAWDPDFNTADLPVLLPISRLDARYARHFMGRKD"
     gene            complement(31960..32415)
                     /locus_tag="PKX01_01800"
     CDS             complement(31960..32415)
                     /locus_tag="PKX01_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HMZ74881.1"
                     /translation="MRLLPLSLALVAVVSVTGCASGLGGGTYERAEARRVMNVRMGVI
                     ESVRVVKLEGTKSPIGTLGGAAVGGIAGSTVGGGKGSSIGAVVGAVVGGLAGSAAEEG
                     LTRKNGLEITLKMDNGEMLAIVQEDEGEGFKPGERVRLLQDGRTTRVTR"
     gene            complement(32427..33362)
                     /locus_tag="PKX01_01805"
     CDS             complement(32427..33362)
                     /locus_tag="PKX01_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HMZ74882.1"
                     /translation="MSTLICGSIAFDSIMVFHDRFRNHILPEQIHILNVSFLVPEMRR
                     EFGGCAGNIAYNLKLLGGDPLIMATVGDDAGPYLERLDGLGLSRAHVTHVPGTYTAQA
                     FITTDLDDNQITAFHPGAMTQSHLNKVSDARDVRLGIVAPDGRDGMLEHCRQFAEAGI
                     PFIFDPGQGLPMFSGEELVACLQLADYCTVNDYEARLMCERTGRTPASLSGELKALIV
                     TRGGEGSEIHTGGQRIDIPCVRPAAVEDPTGCGDAYRAGLLYGIEAGLDWALTGRLAS
                     LMGALKIASRGGQNHTPRRDEIAERFREAFGTTLW"
     gene            complement(33446..34753)
                     /locus_tag="PKX01_01810"
     CDS             complement(33446..34753)
                     /locus_tag="PKX01_01810"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HMZ74883.1"
                     /translation="MILARCPACATTFRVRPEQLRARQGRVRCGHCQHAFNALETLVE
                     NPVPPPPASVDGEPLAEAVESPVFILEEKSVRARHDWTAPDPDSPSPHIEPIGEIPPL
                     PDDAPAEPELLDAADEPTSQTPAFDAAPLADPFPDGSTHTLPERLDDAPPVEPAFILD
                     DPPARHGDGPPDEHLLPLDVDERGAEIARATEPAPIESARDDGASTEPPPAAAFPLPP
                     DFGAPPNDVRLEPEPGAAPDPAPKPFTLVADADDAPFVLPAPQAGPDTSAADDEDAPP
                     RGLRQAVWAAAVTFLIVALLAQGVLVFRNRIALAAPALRPALEALCDRIGCDMPLPRD
                     SSLITIESSDMQPDGSREARFTLYATLRNRADFEQAYPYLEITLTDARDKALVRRVLE
                     PAQWLPPDARKAAFAPNQEISARIVFDAPGVAAAGYQVLAFHP"
     gene            complement(34750..35652)
                     /gene="prmA"
                     /locus_tag="PKX01_01815"
     CDS             complement(34750..35652)
                     /gene="prmA"
                     /locus_tag="PKX01_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMZ74884.1"
                     /translation="MWLSVILQADAARAETLSEALMEHGALSVSIDDADAGTEAERPQ
                     FGEPGMHPASLWDHSRVVALFDPDADLASALSAAAGEAGLDAVPPFHIEEVEEQNWVQ
                     LTQSQFDPIRVTDRLWIVPSWHAAPDPDAINLVLDPGMAFGTGSHPTTRLCLEWLATS
                     LQPGQSVLDYGCGSGILGIAAARLGAGTVTGVDIDEKAVETAADNARNNGVHLTLLHS
                     STALDGQFDRVVANILTNPLKMLAPLLASHIAPGGRIALSGVLEAQADQVIAAYAPYI
                     ALRVADLSDGWVRLEGVRPEQTGP"
     gene            complement(35657..37018)
                     /gene="accC"
                     /locus_tag="PKX01_01820"
     CDS             complement(35657..37018)
                     /gene="accC"
                     /locus_tag="PKX01_01820"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ74885.1"
                     /translation="MFEKILIANRGEIALRVLRACRELGIKTVAVHSEPDADAKYVRL
                     ADESVCIGPASSALSYLNIPALISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRAETIRLMGDKVSAKDAMKAAGVPCVPGSEGALPEDPKEIVKIARSIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVTTTRAEAQAAFGNPVVYMEKFLENPRHVEIQVLADQ
                     HGNAIYLGERDCSMQRRHQKVIEEAPAPGIERRHITRIGERCAEACRKIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELITGVDIVQEQILVAAGQKLRYRQKDIVLRGHA
                     IECRINAEDPFKFTPCPGRITHWHVPGGPGIRVDSHVYNGYTVPQHYDSMIGKLIAYG
                     DTRDQAIRRMRIALSEMMVEGIKTNVALHQDLMRDAKFIEGGTSIHYLEHKLAARNEV
                     KQG"
     gene            complement(37030..37485)
                     /gene="accB"
                     /locus_tag="PKX01_01825"
     CDS             complement(37030..37485)
                     /gene="accB"
                     /locus_tag="PKX01_01825"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMZ74886.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHSAGPAPVAY
                     AAPAPMAINVPAPAAAPAAAAPAAEPALPDGHVVKSPMVGTFYRASAPGAKPIVEVGQ
                     SIAAGEALCIIEAMKLMNEIEADVAGVVKAILVENGEPVEYGEPLFIIG"
     gene            complement(37540..38040)
                     /gene="aroQ"
                     /locus_tag="PKX01_01830"
     CDS             complement(37540..38040)
                     /gene="aroQ"
                     /locus_tag="PKX01_01830"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMZ74887.1"
                     /translation="MTRKNRTQEGTVGELPQTRILVLHGPNLNLLGRREPGIYGLTTL
                     ADIHGAMESRARADGVCLESFQSNHEGELIDRVQAAESEGVAFIIINPAGYTHTSVAL
                     RDALTGVAIPFVEVHLSNIHAREPFRHHSYFSDKAVGVICGLGAQGYQLALEFVLARL
                     RTAPGA"
     gene            complement(38102..38653)
                     /locus_tag="PKX01_01835"
     CDS             complement(38102..38653)
                     /locus_tag="PKX01_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ74888.1"
                     /translation="MTRLTTWSAIAALGLLAAAAGLLTRQHVTDPPSPAPTAAARDAT
                     ARLMALSLPDSAGTQQALQQWRGQVLVVNFWATWCPPCRKEMPEFATLSDKYKGRKVQ
                     FVGISIDSPKNVAEFAAKSPVPYPLLIASPDVITLTEALGNAAQGLPFTAIIDREGRV
                     ARVKLGAFPQDELDRQLAALSGS"
     gene            complement(38650..39267)
                     /locus_tag="PKX01_01840"
     CDS             complement(38650..39267)
                     /locus_tag="PKX01_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74889.1"
                     /translation="MPRPAVPHRKHLRREIAALAARMIAEDGISDYGFAKRKAARQLG
                     AFAADELPNNAEIEAEVRTYLAVFQDEEHLERQLAMRRAALDVMRELEPFRPYLTGAV
                     LEGTAGRYAEVEIDLFPESAKDLDIFLLNQGVPYDHREPRRNQPDGPEAILHLDWDDI
                     PVTLRVYPQDVERLGRRGHGTRPTERARLATVEALVNQTEQTSTP"
     gene            39352..40698
                     /gene="mpl"
                     /locus_tag="PKX01_01845"
     CDS             39352..40698
                     /gene="mpl"
                     /locus_tag="PKX01_01845"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HMZ74890.1"
                     /translation="MHIHILGICGTFMGGVALLARAAGHTVTGCDANVYPPMSTQLEE
                     QGIGLTEGYGVEQLDLKPDVFVVGNAISRGNPLLEAILDAGLPYVSGPQWLAEHVLQG
                     RWVLAVAGTHGKTTTTSLLAWILQDAGLNPGFLVGGVPKNFGVSARLTESPFFVIEAD
                     EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRIMPRAGRIV
                     ANAAEETLQRVIGRGCWSELEWFNDAAGWSVAEGAVEDEVVVRHLGREIGRRRLPLSG
                     RHNRSNALAALAAARHVGVTPEIALDALTRFEGVKRRLELRGTVRGVAVYDDFAHHPT
                     AIALTVGGLRRQVGAARILAVLEPRSNTMKLGTMKDQLPASLTDADKVFCYAAHLGWD
                     AAAALAPIADKSLVTDDFDALIAAIAAEARDGDRILVMSNGGFGGIHGKLLDALAR"
     gene            complement(40716..41759)
                     /locus_tag="PKX01_01850"
     CDS             complement(40716..41759)
                     /locus_tag="PKX01_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMZ74891.1"
                     /translation="MSRINRLVLECALALALVVVSLGAYVRLSDAGLGCPDWPGCYGQ
                     LVVPEQAPDLAAARNAFPDRPVEAPKAWKEMIHRYAASGLGLAILALVAMAWRRRAER
                     TPGLEIALLAVVCAQGLLGMLTVTRLLKPVIVTGHLLGGLSTLALLAVLWLRERPAPS
                     RRPAPAWLRSLALAALAAVFVQIALGGWVSSNYAAAACPDFPTCQGRWWPELDTHHAF
                     TLDRELGATASGALLPGTALTAIHWAHRLFAGVVLGLVGTLGVALMGRVGWRGEGLAV
                     LVALSLQLALGIGNVLLHWPLPLAVAHNTGAALLLCATLAATLRLFTQGAVQTARPAR
                     TTGLFAPPPQPLR"
     gene            complement(41756..42658)
                     /gene="cyoE"
                     /locus_tag="PKX01_01855"
     CDS             complement(41756..42658)
                     /gene="cyoE"
                     /locus_tag="PKX01_01855"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMZ74892.1"
                     /translation="MPALLHAPTALRAPLTLRLAAFVTQCKPRVNSLIVFTALIGMFL
                     AVPGWPPLGRLLAATLGIALVAGAAAAINCLVERTIDARMARTRARPLPLGLITPAET
                     LGLACLTGGAGLWVLHMAVNPLTMWLTLATFLGYAIVYTLLLKPATPMNIVIGGASGA
                     MPPVLGWAAMTGTIGLPALALFLIIFLWTPPHFWALACYRREEYARAGLPMLPVSHGV
                     PLTCLHILLYTVLLAAVSYLPCTLGLSGGTYALLVSGLNLGYLHQAWRLWRDYSDDRA
                     RGAFRYSLIYLTGLFAALFLDRLL"
     gene            complement(42678..43235)
                     /locus_tag="PKX01_01860"
     CDS             complement(42678..43235)
                     /locus_tag="PKX01_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74893.1"
                     /translation="MTARYRPLWLILGLFLLPLAIGGGLFAYGWRPAAPAANGDLLNP
                     ARTLPELLGADGRPLDPATFTDHWTLLVASAGPCEDACRQWIVATRQVHVALYKQMNR
                     VQRVWLTDQPHPDATPLLTLQPDLHTAIAASEAARQRFDLDRAGPQVFVVDPQGRLIL
                     RYAADADPRGLLKDMERLLRYAWAG"
     gene            complement(43232..44005)
                     /locus_tag="PKX01_01865"
     CDS             complement(43232..44005)
                     /locus_tag="PKX01_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HMZ74894.1"
                     /translation="MKTTARSSNGVRRLCAGLLAATSGRRFRPSLAGGVVALAVATLC
                     VQLGHWQAGKADQKQAAQARLDRLGHDAAVDITAAPVDAEVLHGRPVRLRGRFETEYQ
                     FFVDNRVHREQAGYHVITPLRLEGSDRRILVNRGWIPALARHSDVPQVDTPTGPVTLD
                     GVAMVPGHRFFTLGTEAASAGWPAVWQNLDLERFARLAGVPVQPVIVQLDPSAPAGFA
                     REWPRADERYERHLSYAYQWYGFGAAAGLIWFALAWRRR"
     gene            43995..44189
                     /locus_tag="PKX01_01870"
     CDS             43995..44189
                     /locus_tag="PKX01_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HMZ74895.1"
                     /translation="MVFKLAVVLMLVAVIVSLFSGLFFLYRDKGDGARVVRALTIRIS
                     LSVLLFLALLVAYRFGLYSQ"
     gene            complement(44177..45043)
                     /locus_tag="PKX01_01875"
     CDS             complement(44177..45043)
                     /locus_tag="PKX01_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ74896.1"
                     /translation="MNASHASTPHTGHYYVPQPSVYPFILSGGMFLLALGFILLMNRF
                     APGPWVMGGGAMVIIGVLFRWFGAVIAENQGGAYTDWEDKSFRQGMVWFIGSEVMFFA
                     AFFGALFYCRTISVPALAGMDPMFTLYEGYTGGWPTAGPKGASFTPMGAWGIPAINTA
                     LLLTSGMTLTWAHWGLMKGKRAQLNLGLILTVLLGVIFLGFQAYEYHHAYTEMGLTMG
                     AGVYGATFFMLTGFHGFHVTLGAIMLAVVSGRCLAGHFDAQRHFAFEAVAWYWHFVDV
                     VWLLLFVVVYWL"
     gene            complement(45062..45625)
                     /locus_tag="PKX01_01880"
     CDS             complement(45062..45625)
                     /locus_tag="PKX01_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HMZ74897.1"
                     /translation="MTAMHDASLPTSPRHGRLVGRLVLLAGGCFIFGFALVPLYDTLC
                     KAVGFNGKTLKDALSAQEAGATAVDSSRSLNVQFTATVMPGLPWDIRPLEPAFDLHPG
                     ELRTTRFLVRNLSDQPIVGQAVPSVSPTVAASHFRKLDCFCFKQQTLAPGESREMALT
                     FIVDRDIGPDLRELTLAYAFFPAPTPR"
     gene            complement(45764..47368)
                     /gene="ctaD"
                     /locus_tag="PKX01_01885"
     CDS             complement(45764..47368)
                     /gene="ctaD"
                     /locus_tag="PKX01_01885"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HMZ74898.1"
                     /translation="MNTATHALDAHGDNAHHPTGLLRWLTTTNHKDIGTMYMTFALIM
                     FFVGGAMILGVRAELFQPGLQLMKPEFFNQLIGVHALVMIFAALMPAATGFANWMIPL
                     MIGAPDMALPRLNNWGFWLLPPAAMLLTMPFVLALFGIGDGAVATGWTLYAPLSVQGG
                     IGVDFAIFSIHILGISSIMGSINIIATILNMRAPGMTLMKMPLFCWGWLVTAVLLIIT
                     LPVLAGGVTMLLTDRHFGTHFFDAAGGGDPILFQHLFWFFGHPEVYILLLPVWGLMPH
                     VLSTFSRKPVYGHTAQVWSFIAIGVLGLIVWAHHFLTAGLPVPALLYFMYSTMSISLP
                     LAVLFFCWLATMWRGAMSFETPMLFAVGFIVLFGIAGLTGLVLADVAADAQYHHSYFV
                     VAHFHYALFAGGIMGVMTGVYFWLPKWTGHMYSERLGKLHFWLTVVSFNLTFMPQFFL
                     GLAGMPRRIPDYALQFAEFNAISTVGAFCLGLSQLIFAYNVWRCVKGGEPASDQVWEG
                     TDGLEWELSSPPPYHSWETQPVIESR"
     gene            complement(47379..48557)
                     /gene="coxB"
                     /locus_tag="PKX01_01890"
     CDS             complement(47379..48557)
                     /gene="coxB"
                     /locus_tag="PKX01_01890"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HMZ74899.1"
                     /translation="MIPTRLPTLRRPFFAAGIGLALSAPAFADYTWNFPTPVTPIARD
                     TLSMHNQFMVIITALFVVVFAIMIYSMVKHRKSTGAQPAGFSGPSGALQWFWVLVPFA
                     MLLFIDFVLMGIPAFHAVIDMEDTRTKADMVLKVTGMQWKWQYEYPDTGVKFVSTLST
                     PREQIEGKEAKGEHYLLEVDNEVVLPVGKKVRVLLTSTDVIHTWWVPQFGVKRDAIPG
                     FLRETWVRIETPGIYRGQCAELCGKDHGFMPVVVRAVPEAEYVAWLDEKKATQAAASA
                     GSDKTWSKDELMAKGKEAYDKTCAACHQPDGKGVPPAFPALAGSKVVNSPLLSPEGKL
                     IPDSHLDRVLNGKAGTAMQAFKNTLSDTDIAAIVTYERNSFGNAMGDMVQPAQIKALR
                     "
     gene            complement(48592..49068)
                     /locus_tag="PKX01_01895"
     CDS             complement(48592..49068)
                     /locus_tag="PKX01_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HMZ74900.1"
                     /translation="MALTFQEAGGAREWTARPNRSLSSAARRGLIGGLVIVTLLVSAG
                     FMLAGAWMVLPFAGLELIVLALALRALERSDTAYETIRLESGRLSVLTHCPHGDLSAS
                     FHPFWARVSLEPDGPGGDPLLYIRSHGRHARVGHLMTPDQRRTLAAELRGQLQRLP"
     gene            49246..49812
                     /locus_tag="PKX01_01900"
     CDS             49246..49812
                     /locus_tag="PKX01_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ74901.1"
                     /translation="MLLAGCTPSGEGGGKAQGFVATDITGADYARGFRLTDHTGRPRT
                     LEDFRGKVVTIFFGYTQCPDVCPTNLLAMAEVMRQLGDDAARVQVLFITVDPERDTPA
                     LLAQYVPSFDARFLGLYGDPAATAAVAKEFRVFYQKQGDVKGGSYTVDHSTGTYVFDA
                     SGRLRLYVKHGEQPDRIAADLRRLLAGQ"
     gene            complement(49938..50792)
                     /gene="rpoH"
                     /locus_tag="PKX01_01905"
     CDS             complement(49938..50792)
                     /gene="rpoH"
                     /locus_tag="PKX01_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HMZ74902.1"
                     /translation="MTTTLSFPVPSAVGSLDQYIQSVNRIPLLTEKEESDLAHSFRDD
                     GDLDAARKLVMSHLRLVVAIARGYLGYGLPHADLIQEGNVGLMKAVKRFDPTRGVRLV
                     SFAIHWIKAEIHEYIVRNWRLVKIATTKAQRKLFFNLRGMKHDSATLQGDEVLAVAKQ
                     LGVKPEEVREMETRLSGHDVALDAGPDDDEERFAPIAYLADPHAEPSEVLAEQQLARL
                     QDRGLHDALASLDERSRAIVSRRWLSEGDAATLHELAAEYGVSAERIRQIEAKAMQKM
                     RTQLAAFA"
     gene            complement(50977..51765)
                     /locus_tag="PKX01_01910"
     CDS             complement(50977..51765)
                     /locus_tag="PKX01_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HMZ74903.1"
                     /translation="MCIATSVGLGGRNDLADVAAIQILLNLNATRFKPPNPPPLGVDG
                     RIGKNTIQRISDFEVQAMGLPQSDGLIAPGDATVRALLAGLPEGPTKEKLSIVLPLSP
                     AERIDHYFDPLKSGMTKYGITTPLQAAHFLAQVAHESASFLYTEEIASGDAYEGRKDL
                     GNTQTGDGRRYKGRGLIQLTGRANYTAYSKACGVDYVAHPEKIADDPLAAVDVACWFW
                     VTRGLPALAEQDDVKAVTKRINGGYNGLDDRIHYLTRAKAVLGL"
     gene            complement(51921..52979)
                     /locus_tag="PKX01_01915"
     CDS             complement(51921..52979)
                     /locus_tag="PKX01_01915"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ74904.1"
                     /translation="MTFDVRGYAAHSPSDDLVPYRFARRSPRPDDVTIEILFCGVCHS
                     DLHTARNDWRNTVYPVVPGHEIVGRVTSVGPAVTRFRPGDRVGVGCLVDACRHCEACA
                     AGLEQFCQEGPTFTYNSTDRLDRTPTQGGYSERIVTSERFVLRIPDGLDLAGAAPLLC
                     AGITTWSPLRHWQAGPGRRVGVVGLGGLGHMALKLGKALGAELTLFTRSPGKEADARR
                     LGADHIVLSTDAAQMKAVRNRFDLIIDTVPYAHDLNPYLPTLRHDGTLVLVGFLGKLD
                     AMLNTTSLVVGRKAVAGSFIGGLPETQEMLDFCGARGVTSDVEIIRMQDINAAYARML
                     RSDVKYRFVIDMASLKAV"
     gene            53161..54285
                     /locus_tag="PKX01_01920"
     CDS             53161..54285
                     /locus_tag="PKX01_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74905.1"
                     /translation="MNVRKHLAPALLTVLVGAIGGGIWYSNGQLGDEHAQRQLASQTQ
                     ARHVTLRGLIGSEKEAFFNDPRVRQALEAQGIGVTVEKAGSRTIVAQFDAARYDFGLP
                     SGAPDAERLRQVSKAKRVFNPFYTPMVIASWRPIADILVANRIVERQGELYYLTDLPR
                     LLDLIQHGTRWRELKDSGRFSTSKSVLINSTDIRSSNSAAMYLALASYFGNGQQVVQS
                     QADVERVLPVVAPLFLRQGFQEQSSSAPFEDYLALGMGKTPLLFAYESQLVEFWLRHP
                     DRRKGDMVMIYPRPTVYSKHVLVPYTPAGERLGVVLESDPALRALAHEYGFRTGGDVH
                     GPEEWARQGITVPDTLDDVIDPPSQEWQERLIQAIETRFK"
     gene            54299..55549
                     /locus_tag="PKX01_01925"
     CDS             54299..55549
                     /locus_tag="PKX01_01925"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HMZ74906.1"
                     /translation="MTTQNPAVAPAASLDLPPPDAFASLTLEAPAPVAPVPVASAGGK
                     VRLKAEDVHALDQMAGQFIDEITRYDSHDPRFKSAVERIHAMGNRDVEASASVSNRML
                     NRPVQTLKNGMFDKSSLIGANLIDLRRTIEDLDPSRQHDLFKPRKILGLIPFGNTLID
                     YFDRYSSSQSHINAIIEGLKRGRDELLRDNAAIEQEKANLWGLMERLEKYIYIGRKLD
                     GELDAKAAQLDLADPDKARVVREEMLFYARQKVTDLLTQMSVNIQGYLALDMIRKNNL
                     ELIKGVDRATTTTVAALRTAVMVAQALANQKLVLDQITALNTTTGNLIAATSVLLKEQ
                     SQAIHEQAASGTIEIAKLQQAFQNIYQTMDAIDDFKLKALASMQTTVETLGSEVEKSR
                     SYLDRVRRREAGNTFEASSAGVSL"
     gene            55546..57114
                     /locus_tag="PKX01_01930"
     CDS             55546..57114
                     /locus_tag="PKX01_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ74907.1"
                     /translation="MRDRRRGWAAVGAVLLALGLTACGRESGGGAAGTAQGPVTVLAG
                     SELRDVEPQLLKAAADAGVPVKLSYAGTLDIVERVNAGEAVDMILPPSGAYPSLALAT
                     PPRAREKLFYSRVVLGVRSDKARALGWDRRAPAWTDIARAAGAGRLHFAMTNPTSSNT
                     GMSALFAVASAVAGKTEDLSEKEVNGTVLKAFLAGQTLTAGSSGWLAEAYARDPSALD
                     AMINYEAVILRTNASLADKDRLVIIHPRDGVITADYPLMLLNERRRDAYTRLVAAFKS
                     VAVQQNLAASAFLRPALPDVPLAAGVSPEPVVELTFPNRLEVIDAVLQAAQAQWRRPA
                     TSIFVLDVSDSMAGARLDAMRQALLTLVEAKGLAASQRYAGFQPRERVIMIPFSDKVF
                     PPTQVDFGQETLDDGRRQLRTFVNALHIAGGTAIYAALIRADELADAELRQSPDRTVS
                     IVLLTDGINNSGPGYYEFMRHRGADAVRVFPILFGEASVDQMESLASATRGRTFDGRH
                     TALASIFKDIRAYQ"
     gene            57111..57758
                     /locus_tag="PKX01_01935"
     CDS             57111..57758
                     /locus_tag="PKX01_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74908.1"
                     /translation="MNDRSRRPASLRALLFLYSNANIGGCLSALLGPALLFGGVIGPG
                     WMWITAGLYGVGALGGTLLRRTPEVERQIEATLTLDEILQHLDRVIAEVTPEVGREIL
                     THLASLRSAIAEVLPRLLELSGDHGDLYTVRETVLRYLPETLANYVALPPAFRSAHVL
                     RDGKTARQLLTEQIALLDARMQDIVANVASADAQALLANGEFLRTKFDSADFLPR"
     gene            complement(57779..58447)
                     /locus_tag="PKX01_01940"
     CDS             complement(57779..58447)
                     /locus_tag="PKX01_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74909.1"
                     /translation="MSPNDDELSALLNTHATRHRAGPGLQARVRSRILLAGLRGAAGS
                     ALSRAAGPDVLRWRGMAAGFILGVLATLVGVPWVQQIEQDRSAEAAVVASHVRSLGSG
                     PLIEVASADRHTVKPWFQGRIDYAPPVADLADVGFPLLGGRVDTLAGTRVAGLAYTHR
                     KHVINVFVWPDERVAVLRQAQIRGFNTLQWSNGRMGFRVISDLDAGELGRFGETLRAR
                     TAAP"
     gene            complement(58450..58980)
                     /locus_tag="PKX01_01945"
     CDS             complement(58450..58980)
                     /locus_tag="PKX01_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ74910.1"
                     /translation="MSAARRFESLVLPHLDAAYNLARWLLTDRAAAEDAVQEAALRAF
                     RYFESARGDAIRPWFLGIVRNTCFSHLERTRNRQELGGFDEAALDAAHAAAGHSAPEP
                     GRVVEGRQARARIDAALRALPPPLREVIVLRELEGLEYSEIARISAIPVGTVMSRLSR
                     ARERLRAILGALESES"
     gene            complement(58987..59364)
                     /locus_tag="PKX01_01950"
     CDS             complement(58987..59364)
                     /locus_tag="PKX01_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HMZ74911.1"
                     /translation="MKTWTRRRLLGGALSLGAATLLEGLTGRNAVADTPPVIDVEARR
                     FRFTPAEIPLKAGQAVELAIRSVDFVHGFSLPDLGLRTDLPPGRVTRVALPPLPPGTY
                     DFLCDNFCGDGHEEMHGRLIVRA"
     gene            complement(59361..60248)
                     /locus_tag="PKX01_01955"
     CDS             complement(59361..60248)
                     /locus_tag="PKX01_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ74912.1"
                     /translation="MDRRHFLGLSALGGGVVFASGLAGCAGLATAPGATRDFYFVQMS
                     DVHWGYQGEANPDAAITLRKTVQAVNALAVPPAFIVFTGDLTHTTDDPAERRRRMREF
                     KAIVADLQVRTHYYLPGEHDASLDRGAAYREHFGDPNYTFVHEGVHFIALDNVSDPGA
                     RVGEDQLRWLEATLAPLAADTPIVVLTHRPLFDLAPRWDWATRDGAQVMAILQRWRNV
                     TVFYGHIHQEHHRMTGHIPHHSAKSLIFPLPAPGAQEKRTPLPWDAAEPYRGLGFREV
                     HTAAGGSAFGLQEFPVQRS"
     gene            complement(60318..60716)
                     /locus_tag="PKX01_01960"
     CDS             complement(60318..60716)
                     /locus_tag="PKX01_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ74913.1"
                     /translation="MHRLLSLALFGLALCAAMNAHADDAPERGRALYREHCSACHAPD
                     FNGAGPAHRGVVGRLAGTVPGFAYSPALKDAGFVWTPERLDRWLTDPEAVRPGQRMGV
                     SVADAAERADLIAYLRTLGEAPQKETAPRE"
     gene            complement(61349..61774)
                     /locus_tag="PKX01_01965"
     CDS             complement(61349..61774)
                     /locus_tag="PKX01_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HMZ74914.1"
                     /translation="MDARPIPIACALHLLVAGCVLATPLAWAEGEGVRLSRDELQALI
                     PGSETHHTSSAGSVRSWKNAADGSFVASSDNRKYGSAAGTSNATAPGTWNISDDGKYC
                     VQIEWRRVSEKWCSAIVKGDDGAYYLNSVDPAKRIEFVR"
     gene            62045..62464
                     /locus_tag="PKX01_01970"
     CDS             62045..62464
                     /locus_tag="PKX01_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ74915.1"
                     /translation="MTNRSLQYIVKDQEPLTRGPEASLQEACQDMWTRRVGAVMVVDD
                     GRLVGIFTGRDAVHALSRGFDPRHTPLATAMTPAPDTIPPEATAIDALRRMRDCGYRH
                     LPIVDRGRIVGIVSRGDFQGLELDRLEGEDALWERIC"
     gene            62577..65363
                     /locus_tag="PKX01_01975"
     CDS             62577..65363
                     /locus_tag="PKX01_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMZ74916.1"
                     /translation="MAVLFSLLILGVGTAISLYHFGETRHLLDAAQAALFRAVVAEVG
                     RTVDDADRAVRRSFVLLAASSLADATRFGERVRDLPTLTGLLEADPVVDSVMIGYGGG
                     DFLLVRHGRGEVAWEVVDVSRPAGVDAVPVVMVSRYDAHLRLLDARAEDNVSARYEPR
                     LAEWYRLAAAQDALVVTAPGMLPFASRPGVTYARRNGAEVFAVNVGLDNLAGLLARAP
                     VPAGSRLQIADGAGRVVASGGRPDGAGADVMAATLRMSAGSTARRSVHDETGAGWQVS
                     AAPLRGFGEQHWTLAVATPDASLFAEAERQRGHAIRVTVLAMLFSVPLAWGLSRLLTT
                     PLHDLADVARGLSALQFQARPDTRSVILEVDQLARAIGVMRGAIARFLEMGRDLGAAR
                     DVGAVLDDVVNAARDVAGARLCRVEVADDGEGCAEVSRRATGQPLPAGPALQVLSALV
                     YEAEGPVSLADRRTSGPWQYLGIPLRTPDGEALGALLLADRQGPRSGLARAEVAGFLL
                     ALAGSAAVALENQRLLRGRKALLRGVISMVAEAIDAKSPYTGGHCRRVTALAQGLARA
                     AEASSQPSLAQFRLDANGHEALEIACWLHDCGKLTTPEYVIDKGSKLETLYNRIHEIR
                     TRFEVLKRDVEVAYWRGIASGGDAYALRRRRDAAWQSLDDDFAFVARCNRGAEGVSEA
                     DLTRIDRIAARRWVRTLDDRLGLSPEELARHHAPPPPLPVAEALLHDGPEQVIARTDS
                     ALFARGNPWGFTMTVPSRLYDRGEVHNLKVGRGTLTEEERFKIREHIVQTILMLSRLP
                     FPRELAAVPEMAGGHHETPDGRGYPRGLRGDQMSVAARILAIADIYEALTAPDRPYRS
                     ANTPAEALEIMTRLADEQAIDEALFRVFVEAQVWQVDDRPVAPASGLSAIGQQAGQHD
                     KQDECDPQCPADPS"
     gene            66006..>66227
                     /locus_tag="PKX01_01980"
     CDS             66006..>66227
                     /locus_tag="PKX01_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HMZ74917.1"
                     /translation="MAAQTQRIRLTVDRIRRLEPPTGKQAIYVFDDDQRHLAVRVTPA
                     GAKSFVFAGKLNKVPLRITIGDVMVWQLDD"
BASE COUNT        10067 a        20936 c        24676 g        10548 t
ORIGIN      
        1 aatggatcag ggccgtgccc accagccagg gcatgaagga ggcgttctcc accggatccc
       61 agaaccacca gccgccccag cccagttcgt aataggccca gatggagccc aggccgatgc
      121 cgagggtgag gaagatccac gccgccgtgg tccacggccg cgaccagcgc gcccaggccg
      181 catccaggcg acccgacagg agcgcggcga gggcgaaggc gaaggccacc gagaagccca
      241 cgtagcccat gtacagcatg ggcgggtgga agatcaggcc cgggtcctgc agcagcgggt
      301 tcaggtcgcg gccgtcttcg gcgcccggca gcaggcgctc gaaggggctg gaggtggtca
      361 ggatgaagag gatgaagccg gccgccacca gcccgaggac cgcctgcacg cgggccacca
      421 tgtcctccgg caggctgcgc gagaaggccg ccaccgccag cgaccacagg gccagcatct
      481 gcacccacag cagcagggag ccttcgtggc cgccccacac gccggcgatg cggtattcga
      541 cgggcagcct ggagttggaa tgctggacga cgtagagcac cgagaagtca ttgttgatga
      601 agctcgccgt caggcagctg aaggccacgg cgaccagcag aaacagggtg gccgacgccg
      661 gtcgggcgag ggcgatccac gccgggatgc cgcgctgggc gcctaccagg ggcagcacgc
      721 cctggacgag ggcgaccagc agggcgagga cgagggcgaa actgccgagt tccgggatca
      781 tgaggatgcc tccgggctta ctgcttgagg gacttggcgg cctggtgggc ctggtccacg
      841 gccgccttgg cttccggcgg catgtagttt tcatcgtgct tggcgaggac ttcgctggcg
      901 gtgaactggc cgtccacccc caggcgtccc tgggccacca cgcccttgcc ttcactgaac
      961 aggtcgggaa ggatgccggt gtaggtcacc gggatttcct ttgcggtgtc ggtgacggta
     1021 aagcgggcgg tgatgtccgt gcgctgcagg ctgccctcct tcaccagccc gccgatgcgg
     1081 aaagcccggt ctttgggcgc ctctccggcg gccacctggg tgggggtgaa gaagaacacc
     1141 agattgctct ggaaggcgtt gagcaccagg gtggcggcga tgcccagcgc ggcgaggccg
     1201 ccgaggacga tggcgaagcg tttgtgtcgg gctttcatgg gtgatcttga ctctctctgt
     1261 cggcggccag ttcccggcgc agggcgcggc gggcggcccg gaagcgggcg cgggtcagca
     1321 gggcttcgat cagcagggcg ccaaaggtga cgccgaagct gccccacacg tagaggccat
     1381 agccgcccat ggcgaagaat tcactcgcgc tgccccagtt catgcctgca tctccggcag
     1441 ttcggccacc caggcggcat ggcgttcccg ttcgaggatg atggcgcgca cgcgatacag
     1501 ggcggtggcg atgctgtaca tccagaaggc cagggccatc agcagcatgc cccacaacat
     1561 ggtggtggcc atggacgggg ccttggtgag ggacacggac gcgccctgat gcagggtgtt
     1621 ccaccacttc accgagaaat agatgatggg cacgttgatc acgcccacca gggagatgat
     1681 cgcgccggcc ttgtcggcgc ggcgcgggtc gtcgatggcg ctggtgaggg cgatgtagcc
     1741 caggtacagg aagaagagga tcagctccga ggtgaggcgc gcgtcccaca cccaccaggc
     1801 gccccacatg ggcttgcccc acagggcccc ggtccacagg gacaggaagg cgaacagcgc
     1861 gccggtgggc gccagggcgg aacacatcat gcccgacagg cgggtgttga gggagatgcc
     1921 caatgccgac cagaaagcca tcacgcagta gatgaacatg gacatccacg acgccgccac
     1981 gtggatgaag atgatccggt agccttcgcc ctgctgggca tcggtggggg cgacgaagaa
     2041 gccgagccac agccctgccg ccgcaaatgc cgccgccagg gcggcaaacc agggccaggc
     2101 ccggccggcg agggggtaga agctggccgg cgaggcgaac ttgaaccagt tgatgagacg
     2161 ctgactcatg gaggggttcc gcaggaaacg ctattcgagg gcgatgcgca gggccgccgt
     2221 ggccgcccac ggggcgaaga acgcgccgag ggccagcacc gcgcccagca gggacaggtg
     2281 accgccggcg ccaagcccgg ccatctcggc ctcgaccgcg ccggcaccga agatcagggc
     2341 tggaatatac aggggcagca ccagcaggga cagcagcacg ccgccgccgc gcacccccag
     2401 cgtgagggct gcgccgatgg cgccgatgag ggacagcacc ggcgtcccca gcagcaggga
     2461 cagcatcagg atgcccagcg cctggggcgc caggtcgaac tggatcccca gcaccggtgc
     2521 cagcagcacc aggggcaggc cggaggtgag ccagtgggcg acgatcttgc cgcccaccag
     2581 cagggccagg ggcacaggcg acagggccat ctgttcgaga gtgccgtcct ggtggtcggc
     2641 ggcaaacagg cggtgcaggg acagcattgc cgcgagcagg gcggccaccc acagcacgcc
     2701 gggggcgatg cgccgcagca tggcggtttc cgggccgatg ccgagcggga acaggctcac
     2761 cacgatgagg aagaagaaca gggcggtgag cacttcgctc ttgcgccggg cggccagcag
     2821 caggtcgcgg cggacgatgg cgatgaaggc gttcatgcgc ccagcctcag gctgccgccg
     2881 gcgccgctga gctgcaccgc ctggtggctg gtgaacaaca gcatgccgcc gcgccccagg
     2941 tggtcgtcga ggatggcgca gagctggtcc accgctgcca cgtcgagggc cacgaagggt
     3001 tcgtcgagaa tccacaggcg ggcctggctg cacagcagtc gggccagggc gacgcggcgc
     3061 ttctggccct gggagagaac ccggctgggc aggtcttcgc gccccttcag gccgatgcga
     3121 cgcagggcac cgagggcggc ggcctcgtcc agaggtcggc cggccacggc ggcggcgatg
     3181 gtcaggttct cgagggcgct caggtcgtcc ttgatgcttg gcgcgtggcc caggtagagc
     3241 aggcgggcgt ggtattcgtc gcgcacgtcg gccaccggcc ggccttccca ctcgatgtgg
     3301 ccggcggcgg ctggcgcaag gccggcgagg atgcgcagca ggctggtctt gccggcgccg
     3361 ttggggcctt ccacctggag ccattcaccg ggcgcgacgg cgcagtcgac accggaaaac
     3421 aggcggcggt caccgcggtc gcaggcgagg ccgtgggcac ggagcatcgt gatcgggggg
     3481 aaagtggagg ccggggcgaa ttctgcccga cgtgggcacc ccggtaaacc gccggggggt
     3541 gggcgggcgg cgtgcgcggg tgtctgtcac gcccgcaggc acgtcacccg gacctgttcc
     3601 gcaggtgggc gcggcggatc gggtggaccg tcgcggatgc ggggggcggc ggactcagtg
     3661 atgggccgtg ctggcgtagc cggccggcag gacggtgggg gcgggcagcg agtgggcggc
     3721 ttcgtgtgca tcggtgccca gggcgtcggc gtcggtttcg agcacgtcca tcacctggcg
     3781 ggccagcgca cggcaggcgt tgcccaggtt ggagggcaga ctggcgggga tgtgcttctg
     3841 gagcagcagc ttggaggcgg acaggctggc caccgggtgc agcagacggt cccggtggtc
     3901 gcccagcagt tcgagcacgg cccggtcggc ggcgtcgcgc tgccaggcgt tggtggccca
     3961 ctgggagggc aggccgtagg cgcgggggaa ggtttccagg tcgcggcgga cggtgcgcat
     4021 gtcttcgtgg gagtcgcgga agggcagcaa cacaatgtcg gcgtagctgt cgaggaggcg
     4081 gtccacctcg ctgcggttgc cgccgccgtc gatcacgccg atccagtcct gcatggtgcg
     4141 cagcttgccg acgaccttgt gaagggcttc cgggctgcgc ccgtcggcgg tgacgtagcg
     4201 ccgcccgtcg gggctgaggg gctcgcggga gacgtcggtg aggacgcagg cggaacgctg
     4261 tccgagcagc ccgaaaccct ggcagagcat gtgggacagg gtcgtcttgc cggtgccgcc
     4321 cttgttgccg atgatgcaga tgatcgtgga catggtgaaa acctctcgca gaacgcacgg
     4381 cgcggtggcg ccattattcg tgccctaacg gcacggcgag gggtggaatt gaggggcgtt
     4441 ccgggggtaa ttccggacca gcgtgcctca ggggaggaga ccgaccggag acccgacggc
     4501 acggccgtcc ggcggcagcg gtccggcggg gtggtagcag tagaggcggg tgccgcggcg
     4561 gtcggcgacg tggatgatgt cgtcgggctc gatgccctcg accggaaagc tgttgctcac
     4621 cagcagggcg ccggggcgca gctcggcgca ggccttgcgc cacacctcgg gcatggggac
     4681 gggggacagg aaggcgtaga tcaggtcgta gtcggtccac gggatctgcc agaaatcgcc
     4741 tcgcgccagg gcgatattgg ggtggcggcg ggacaggagg cgggacagca gatagggcgc
     4801 cggggcgctt tccatgcctt ccaggcggca gtccgggcgc aggcgggcga ggggccgcag
     4861 cagggcgccg gtgccactgc ccaggtccag cacgcgggcg gaatgaccgg gggggacgag
     4921 ttgcgcgacg gccgctgcgg tggccggatt ggacaggaac aggggcacct gggtgcggaa
     4981 gctgctccag tacaccagcg ccagcagcgc aaaggccgcc agataccagg ccggatgcag
     5041 acccagttgc agcgccagcg ccgccagcgg cgtaaagccg gcgtggatcc agcgccacca
     5101 gggggcgctg cgcagggcga cggcggcgct caccgcagca atggcttggg ccagcaccag
     5161 ggcccaggcc tggccgggca ggaggccggc gcgcagcagc ccgatggcga cggcccagcc
     5221 ggcgagctgg gcaaggaggg ctttgagggc agggggaagg cgcatcggat gactgatcgg
     5281 gaggctgccc ctgcagtgta gggtgaaacg gcccggcggg gggcgcagcc actcagagca
     5341 ggctgcggcg ggccagcagc aggatcagga gcacggtctg caaggcgcac aggatctgcc
     5401 agaacagcaa gggattgcgc tcgatcaggg ggggcgggcg ctggcccagg agggccgcat
     5461 tggggtggcg caggtcgtag gtgaattcgc tcagttccgc gtagcggcgc aggggatcgg
     5521 gatggaccgc cttggccagc gcggcgtcca gccaggccgg gatctcgcgg gtgtcgctga
     5581 ggacgctgcg gtagcggagc ttcttctggg cgctgcgggt gcgcgcctgg gacacgtcgg
     5641 tgccgtaggg caggcggctg cagagcatct ggtaggcgat gacgcccagg gagaagaggt
     5701 ccgagcggga cgtgccgggc tcgccgagga agtattcggg cgcggtgtac tggcaggtgc
     5761 cgaggatggg tgccagggtg tcgttggcgg cggtttcctg caggccgttc acgtgggtgg
     5821 cgccgaagtc gatgatgcgg acgacacctt gcgtgtcgat catgatgttt tccgggcgca
     5881 ggtcctggtg gagcatctcc aggcgatgga aggccagcag gccgcgggcg atctgttcga
     5941 cgatgccgcg cacggtctcc acgtccggcg cggggtggtc gcgcatccac tgggccaggg
     6001 tgcagccttc gatgtactcg gtggcgacgt agaggtagct gcgctgccgg gtaaccgggc
     6061 agggcttgag cacgtggggg ctgtcgaggc ggcgggcgat ccactcctcg agcatgaagc
     6121 gctcccggta ggccttgtcc tcgcgctggt cgatggacgg gagcttgagg gcgacgcggg
     6181 tgccgctggc gatgtcctcg gccaggtaga ggtggctgcg gctgctgccg tggagcgtgc
     6241 gccggatgcg gtagccgtcg aattccatgc gggcgtccag cagcggcggc aggggcagtt
     6301 cggcgaactg ctggaggtgc tcggcggcgg cctggacggg cagggtgtcg acgcggatga
     6361 gctggatcgt gaggttgtcg gcactgccct gggcaagggc tgcgtcggcg atgcggcggg
     6421 ccgccgcgtc caggtcgggg gctgcgcgca gcgtggcgcc gatgacgtcg gcgccgacgt
     6481 gttcgtgcac gccgtcggtg ctcaggacga agacgtcgcc cgccgtgatg gggacctggc
     6541 ggtagtccag ttccagttga gcctcgaagc cgagggcgcg ggcgaggtag ctctgctcgg
     6601 gcgagacgac gatgcggtgg gcgtcggtga ggacctccag gcggccgtcg cggaggcggg
     6661 cgatgcggct gtcacccacg tggaagaggt gggcggtgtg ggacttgagc accagcccgc
     6721 tgaaggtgca gatgtagccc cgatccttgt cgtagcggtg ggggctgttg cgggtctggg
     6781 cgtggagcca ggcgttggtg gcctccagca cgcgctgggc ggaggtgcgc acgctccagg
     6841 tctcggcggt gcagtagtag tcgccgagga agctcttgac cgccgattcg ctggccagat
     6901 ggctgacttc gctgctgctg atgccgtccg ccaccgccgc cgcgatgccc ttggtggtga
     6961 gctgcggctc cacggggatg cagagcccgt ggaagtcctg gttggcgggc ttgcggccct
     7021 tgtccgagta ttgcccgacg gtgacggtga gggaggtcgg catggacggc tccggcgcgg
     7081 cgtccgcgcg aggcggacgc cgggagggac ggcggatcag gcgttgcgct tgggggccgt
     7141 gcgcacgtgg gtggagtaga gggtcaggcc gacgaaggtg atgccgccca ccagattgcc
     7201 caccacggtg gggatttcgt tccacaccat gtaatcgtac agggagaagt tgccccccag
     7261 catcaggccg gaggggaaca ggaacatgtt gacgatggag tgttcgaagc ccatgtagaa
     7321 gaacaccatg atgggcatcc acatggcgaa gatcttgccg gtgacgtggg tggacatcat
     7381 ggctgcgacg acgccggtgg acaccatcca gttgcacagc acgccgcgga tgaagagggt
     7441 cagcatgccg gcggcgccat gggcggcgta gccgacggtg cggctctcgc cgatggtgcc
     7501 gatcttctgc ccgatggcgt tgggcgcctc ggtgaagccg aaggtgaaga tgatggccat
     7561 catcaccgcc accgtcaggg cgccggcgaa gttgcccacg aagaccagcc cccagttgcg
     7621 cagcaggccg ccgacggtga cgccgggacg cttgtccagc agggccaggg gcgccagcgt
     7681 gaacaccccg gtgagcaggt cgaagcccag cagatagagc atgcagaagc ccaccggaaa
     7741 gaggatggcg ccggccagcg gctggccggt ctggacattg atggtgaccg cgaaggctgc
     7801 cgccagggcc aggatggcgc cggccatgta ggcgcggatc agggtgtcgc gggtggacat
     7861 gaagattttc gattccccag cgtccaccat cttggtgacg aattcggtcg gggcgagata
     7921 ggacatggtg cgtctcctgc ggtcgtgaag attccggaat ggcgtttccg catcgacagg
     7981 caatgcggaa acgaaaaacg gcgtccgttt cctcccgcgg tgcgggacgg aaatggacgc
     8041 cgttatccat caaacaaagg aacactgaac cagcgttggt tcagctctgg agggtgataa
     8101 agcaggtttc acgccaaggc aaatgagggc ggcgctgcgg gttttcgcag ggcatggatg
     8161 gcagaatgcg ccccgattgg gtgcggcgct gcggatcagg cacgttattg gtgcgtagcg
     8221 tgtcaggcgc ctgtgcatgc gccgcggaaa ggtgcgtcga cccgcgcggc ctcagtcgat
     8281 gaagaccgac atcacgccga acacggcgta caggagcatc cacggaaagc tcacgacgaa
     8341 gaggctgttg tggggcccgc ggggcggata gtgtccgccg ctgagggcca gcagcaatgc
     8401 ccggccgagg ggatactgga tcacggcgac gcccggtgcg atgaggtggg tggcccaggg
     8461 cgcgcgccac ggattggggc cgcgggcgcg cttcgcgagc aggcgctcgg cttcggcccg
     8521 cgctgtgtcc aggctcggga gcgccttgga gatcagctgc tcgccgtcct ggacgaaata
     8581 gcggcgcgcc tgcgcatcgc ggccgccgat ggacacgatg tgcaagccct tgtggtcggt
     8641 gatcggttcg agcatggcgg ttccggtcga tgcgggcggc atgagtatag tcgaggcgcg
     8701 ggcgccgccg gtcgccatgt tctccggctg gcggttcagg tgccggaggg gcggcgcagc
     8761 gcgcgcaggg cggcgagttc tgcgagcagc gcggtccggc gttgcgccgc ctgggccatg
     8821 gcgtcggcct tcacgtggcc atagccgcgg atcgtctcgg gcaggcgggc gacggcttcg
     8881 gcgacgtgga gggtggtggc gctgaggtcg ctcagcaggt cggcgatgtc ggcctcgtag
     8941 tgcgcggtga gggcgcgttc ggccttgcgt tccgggtggt aggcgaagag gtcgaagggc
     9001 gtgccgcgca ggcgtcgcag ccaggccagg ccgcggaagg ccgtcatcag ccaggggccg
     9061 aaggcgagct tgcggaggcg tccggtggcc gggtcggggc gggcgaggaa ggtgggggcg
     9121 agatggaagc gcagccggta gtcgccctcg aaggcggcgt ccacggcggc gcggaaggcg
     9181 ggcgcggtaa acagccgggc gacttcgtac tcgtccttgc aggcgagcag ccggaaatag
     9241 ccgtcggcga cggcctcgcc caggcgttcg ctgccgaggg gcgcttcggc ggtccgcacg
     9301 cgggcgacga aatccgtata gcggcgggcg taggcggcgt cctggtagtc ggtaaggaag
     9361 gcggcgcggc gggcaatgcg ggcgtcgagg ccgtcggttt gggtgtcgtc ctgcggtgtc
     9421 gccgccgggc gggcgagggc ttcgacggcg gcccgatcca cggcactccg gcggccccac
     9481 aggaaggcgg cgcggttcag ggcgacggcg gcgccgttga gttcgatggc ccggtcgagg
     9541 ctggcctcgc tgaccgggac ccagcccttc tgccaggcgt agccgagaag gaacaggttg
     9601 gtggcgatgg cgtcgccgag caggcgcgtg gccaggtcgg tggcgtcgag gaagtcgcag
     9661 gcgtcggcgc ccaccgcttc gacgagggcg gcctgcatgc gctcgagggg gaactgccag
     9721 tccgggttgc gggtgaagtc ggaggtgggc gtctcggcgc aattgacgac gccgtggctg
     9781 cgtccgacgg ccatcttggc gagggcgtcc acgctggcgg tgaccaccac gtcgccgccg
     9841 atcacggcgt cggcctcgcc ggcggcgatg cgcacggcgt gcagcgcctc gggccggtcg
     9901 gcgatgcgga tgtgactgaa gaccgagccg cccttctggg cgaggccggt catgtcgagc
     9961 acggtgacgc ccttgccgtc caggtgggcg gccatgccga tgagggcgcc gatggtgacc
    10021 acgccggtgc cccccacgcc ggtgacgagg atgccccagg gccgcccggt gggcggcagg
    10081 acgggggccg gcagcgccgg gaagcgggcg tcggtggtgt gccgggcgct gccgcggcgg
    10141 agggcgccgc cttccacggt gacgaagctc gggcagaaac catccatgca cgagaaatcc
    10201 ttgttgcagg cggactggtc gatgcggcgc ttgcgcccga attcggtttc caccggcagg
    10261 atcgccatgc agttggaggc gcggccgcag tcgccgcagc cttcgcagac ggcttcgttg
    10321 atgaagacgc gggtctgcgg gtcggggtag gtgccgcgtt tgcggcggcg gcgcttctcg
    10381 gcggcgcagg tctggtcgaa gaccagcgcg gagacgccgg gcacactgcg cagctggcgc
    10441 tgcagcacgt ccagctcgtc ccggtgatgg atcgtgacgc cgtgggggat gtccgccacg
    10501 tgccacgaac gcgcggtgcc gtcggtgacc agggcgatcg tgtgcacgcc ttcggcctgg
    10561 agctggcgca ggagggcggg gacggtgagg acgccgtcta cgggctggcc gccggtcatg
    10621 gcgacggcgt cgttgtagag gatcttgtag gtgatgccgt gggtcgcgcc agggcggttg
    10681 aaggcggcca cggactggcg gatggccagc aggcccgagt ggaaataagt gccgtcaccc
    10741 aggttggcga aaatgtgacg ttcgtccgtg aagggggcct ggccgaccca gggcacgcct
    10801 tcgccgccca tgtgggtgaa ggtgccggtg cggcggtcgg gcatgaagct gaccatgtag
    10861 tggcagccga tgccggccag gccgcggctg ccttcgggca ggcgggtgga ggtgttgtgg
    10921 gggcagccgg gacagaaggt gggcacccgg tcgagggcga agacccggtg ggcgagggcg
    10981 tgttccttgg cgtcgaggaa ggcgaggcgt gcctggatgt gttccgaggt gaagaagcgc
    11041 ccgatgcgcg cggcgatgac gcgggcgatc atcgccgggg tgagttcgcc cgccgccggc
    11101 agcagccagt cgccgtggtc caccgtcgca cgaccgtccg gcccgacggt ggtgaggagg
    11161 gcccattcgc ccttctcgtc gaatttgccg acgacccgcg ggcgcacgtc ctcgcgccag
    11221 ttgtagagct cttccttgag ctggtattcg aggagctggc gtttctcctc gatgaccagg
    11281 atctcgtcca gaccttctgc gaagcggcgc acgccctcgg cttccagcgg ccataccatg
    11341 cccaccttgt agaggcgcag gccgatgcgc gcggcgaggg tctcgtcgat gcccaggtcg
    11401 tcgaaggcct ggcgcacgtc caggtagctc ttgccgctgg tgaggatgcc caggcgcggt
    11461 tcgggcgagt cgatgaccac ccggttgagg tcgttggcgc ggcagtaggc gagggccgcg
    11521 tagagcttgt agtggagcag gcgcctttcc tgtaccaggg gcggatcggg ccagcgcagg
    11581 ttgaggccgt ccggcggcag ggcgaagtcc tcgggcagac gggtgacaac gcgcagcgga
    11641 tccacgtcca ccgaggccga ggtctccacc gtgtcggcga gagccttgaa agccacccag
    11701 cagccggaat agcgggacat ggcccagcca tgcacgccga aatccaggta ttcctggacg
    11761 cctgccggcg ccagcaccgg catcatcatg gatttgaaga agtggtcggt ctggtgcggc
    11821 agcgtggagg acttggccgc gtggtcgtcg ccggcgatca ccagcacacc gccgtggcgg
    11881 gcgctgccgg ccgcgttggc gtggcggaac acgtcgccgc agcggtccac gcccggcccc
    11941 ttgccgtacc acatggcgaa aacgccttcc acgcgcgcgc cgggggagag gctcacctgc
    12001 tggctgcccc acaccgcggt ggcggccatg tcctcattga ggccgggctg gaagacgatg
    12061 tcgtgaccgg cgaggtgggc ctgggccttc cacagggtct ggtcgagccc gccgagcggg
    12121 gagccacgat agcccgagac gaagccggcg gtcctgaggc cggcggcgcg gtcgcggtca
    12181 cgctggatca gcaggaggcg cacgagggcc tggtagccgg tcagatacac gcggccggtg
    12241 gcggcggtgt atttgtcgtc gagtgtggcg ccgggcgggc gggcgcggac gggatcctgt
    12301 tcggccatgg tgaacctcct tgcgcaaaat catgttcggg ctgcgtccgg ggcgttaggc
    12361 ttgtggcctt tgccatggag gtgtcgcact tgcagcgtag tctggaaggc cctgccgctc
    12421 aaaccttgga acgcaggctg tgccacaggt tgcacgacgg ctgcggtttt caccgatgtg
    12481 ccggcaggcc gtctgcatga tgcttacaaa cagaaaagat tttttcgacc gcaagacttc
    12541 tcctttccgg ccttccgggc gttaatgggc tgaattgccg ctgcggcccg cgtctgcgct
    12601 gcccgaccct ggacatacac catgaaatcc ctgatccccg tctcctgcct catcctgctg
    12661 tgcggctttg cccaggccca ggcgagcgaa gcccacacgc ttcgcgctac gcccgccgat
    12721 cgggcggaca cgtgcgccca cggcggagag cgtcacgggc tggaatacgc ccactggaac
    12781 gaatacctgg ccacccgcgc cggcgacagc cagagcgccc gcgactgggc cgagcgcgcc
    12841 cgcgccgagt gccgggagct ggcgagtgag cgtcgcgacg gccccgagcg gcccgaagcg
    12901 cggccggacg tgcgccagga acgtgcccgg cggcgggaac tggcgcggcg ctgacccgct
    12961 tcggccatgt cacgtcggcg cattcccgcg acgcccgagc cggcccgcgg accgtctccc
    13021 cgccagatcc tgaatcggct gggattcggc atcggtccgt ccgacgagct cgcccggctg
    13081 gcccgcgacg gcctgccggc gtggctcgac gcgcaactgg cgccgccgcc cggtgacgac
    13141 ggcgaatgcc tgcagcgcct gggcgccgca cgactgcgca tccgctacga cgcgggcaag
    13201 gcgccgggac cggaataccc ggccgtggac gagaaccggc cgctggagac cctgcaggcg
    13261 cccatcgaga ccctgtggcc gcgcactgat tacaagcgcc cgatggccgg gtcggagcgt
    13321 ttccggccgc gggccgaggt cgcggctgca accctgatcc gcgccgtcca cagtcgctgg
    13381 tcgctgcgcg aagtgctggc ggatttctgg cacaaccact tcaatgtaga tgcctcccag
    13441 atcgtgcagg tggcggtggc cctgcccgcc tacgaccggg acgtgatccg cgcccacgcc
    13501 ctgggcaact tccgggaatt ccttgaggcc gtcgccacca gcacggcgat gctgtattac
    13561 ctcaataatc gttcctcgcg tgctggcgcc gccaacgaga actacgcgcg ggagctgttc
    13621 gagctgcaca ccttcgggcg cggcgcgtac ctgaacgagt tctacaaccg ctggcgcgat
    13681 gtgcccggcg ctctcacggg gcagcccaag ggcttcatcg accaggacgt ctatgaggcc
    13741 gcccgcgcct tcaccggctg gacggtggag gacggcagcg gcctgggcgg cagcgaccgc
    13801 ctgccggcca ccggccgctt cgcctacgta gacagctggc acgacaacta ccaaaagcgc
    13861 gtgctggcca ccgagttcga cccctttcgg ccggcgctgg aggatgggcg caaggtgctc
    13921 gacctggtgg cggcccatcc ggccacggcg cgtttcgtga gcggcaagct gtgccgccgc
    13981 ttgctgggtg tcgagccctc ggccggaatg ctcgcggcgg cgtccgagac gtggcgcgcg
    14041 gcgctgcggg cgccggacca gatcgcccgg gtggtgcgca ccatcgtgct cagcccggag
    14101 tttgcccgtt cggcggacct gcgggtgcgg cggccactgg atctggtggc ggcgctggtg
    14161 cggggggcgg gcatggactt cgtgcctacc gaagggctgg tcaacgagat ggcggccgcg
    14221 gggcagcgtc tgtttggttg gggcgcgcca accggccacc cggaggaggt tgattactgg
    14281 ttgtcgtcga acggcatgcg gcgccgctgg ctgctggtgc agggactcat cgagaaccgc
    14341 tggggcaacg gtgtggtgga ggttcgccca cccaccccgg cgtccacctg gcgcgaggcg
    14401 gccgatgcct gggccgagca cctgctgggt gaggcgccgg ggagtacccc cgtcgggcag
    14461 atcctggccg gcctgcgcca ggacggggct acgcccttcg atccgggcaa accggcccag
    14521 gcagctcaga tgcggcgggt actgggttac ctggccatgg ggccgggctt ccagcgggga
    14581 tgaggacatg acgacacggc gcagctttct caaagcctgt gcggcggcgg gcgccctgat
    14641 gcccggcctg ggcatcgggc gcctggccta tgcagcgacc gacgcggcgg cgccgttgac
    14701 ggtggtggtc ttcctgcgcg gggggtgtga cgggctactg ttggccgggc ccgccaacga
    14761 gccggcctac gtggaggcgc gtcccccgga gctgcgggtg ctggatggcg gcgagcgggc
    14821 gggcctgccc ttggccaacg ggctgaccgc ggatctggat ttccggctcc atccggacgc
    14881 gcgcccgctg tacgagctct atcaggcggg gcgcctggcg ctggtccatg cggtcggcct
    14941 gaccaacggg acgcgcagcc acttcgaggc ccaggatctc atcgagcgcg gggtgagcac
    15001 gctggaacag gcgtatcgca cgcccgaagg ctggctgacc cgctacctga ccctgtacgg
    15061 cggcgcggac ggcagtgccg accacctcgc ggccctggcg gcaggcgcgg gtctgccggc
    15121 ctccctggcc cgccagggcg acgccctcgc cctgcctgat ctgcagggcg gcgtcggact
    15181 gcccggtggc acggacggcg cggcgctgct cgggcaactc tacgccgcgg gcgattcgcc
    15241 ggtgcatgag gccgggcgcc gggcgctggc gcgcctgtcc caggtggatg cgcatctgcc
    15301 gcgcacgccg gacggcaagc tcgcgccata tgcgcccgac ggcgcaggct acgagtccgg
    15361 cgcattcagc ctgggcctgc aggccattgc ccgggtggcg cggatgaacg tcggcctgag
    15421 ggtcgcgtgt atcgaccacg ccagttggga cacccacgac gcggaaagcg ggcgcttcgg
    15481 cgggctggtc ggccagttgt cgcgggggct ggctgcattt caccgggacc tagccagcgc
    15541 ggggattccc ttccggctgg tggccatgag cgagttcggg cggcgtctgc gggccaaccg
    15601 cagccagggc acggaccacg ggcatggcgg cgcgatgctg gtgctgggcg agggcgtgcg
    15661 cggcggccgc atgctggggc gttggccggg gctgagtacg ccggccctcg atcaaggggt
    15721 ggatctggcc gtcaccaccg attggcgtgc agtgctggcc gagctgttgg ccctcgacgg
    15781 gcgcggacgc gggctggtgt ttccgggcct gccggtcgaa gcacctcttg gtgtgctggg
    15841 ctgaggcttt gctccggtgc ccggccggcg tcagaacaga tccacctcgc ccgcatgcac
    15901 ggcggtctgc tggaccgcct cgaagcggat gtccttcttc gactgctcgg actgggccag
    15961 cagctggcgc aggatctcca cgcggcgctg gatgcgggag gtcgcctcgc gctgttcggg
    16021 atcccggtgg gcgtcgaaga agccgtgggc gtaacgcccc atgaagtcgg tgtgctggcg
    16081 cagcttgtcg aggatctggc ggacgatgtc ctcgaactgc atggactgga tgccttccat
    16141 caccaggcga tggatcttgg cggagagttc gctgatgcgg cgcgactgaa ccatggcgcg
    16201 ggcgttcagt tcgctcatct ccagccccat tgcggacacg ctcgcttcgg atgagcgcgc
    16261 cttgctcacg tcggtggcgg ccgtgacatc cacggcgcgg cccacgtcgt cgatggaggc
    16321 gtggatgtcc gtgaggaggg tcgcgatctg atcgctgaag gtctcggtgc gctgggccag
    16381 tttgcgcact tcgtcggcga cgaccgcaaa gccgcgcccc gcctcacccg cgcgggctgc
    16441 ctcgatggcg gcgttgaggg cgagcagttc ggtctggctg ttgatctgat taacctcggt
    16501 catcaggcgc gtgaccgcat ccaccttgcc gcgcattgcg ccgaagcgct cggcgatgtc
    16561 gctgccgctg gccttgaggc gctccacggt accgacgaag tcgcgcaagg cgtcgcgggt
    16621 ctccgcggcg aagcgctcca gcccggattc ctgcagggcg cgctcttcct cggcggcgag
    16681 ggacagcagt tcgtccacca gatggcgcag ctggtcgcgg gaattgcccc cctggccgcc
    16741 ggcgaggctg ccaccgagct tgctggctgc gctgctcacc acgtcctgca tctgccccag
    16801 ggattcccgc aggcgttcgt actggaagcc gctctggacc gcgatctgat cgcacagcat
    16861 gtcgtattcg cgcacgctgc tgtcgatcga ctcccgctgg gcctgctgtt gctgacgcca
    16921 gcgttcgcgg gcgcgccggc tgacgatcat ggccgtgacc agggccacca ccgtgaaggt
    16981 gccggcaacc agggacaggc ccgcggggcc gccgagcgcg gccgaggcaa tgccgcccgc
    17041 gctggtggcg agggcgacga gtatggcgaa gtcgtcagcg cggaagcggg tccggatcat
    17101 gggggaagat catttcgggt gagtacgtcg tcgactaacg gcagcatgca gcccgacttg
    17161 atcgtctgat atgacgaaag tcataggtca gggcatcgtc gggcccttcc cgtaaggcat
    17221 cgcaacgacg tgcgcctggg gacgaacggg cctgcgggcg tgtcggaccg gcggtttcag
    17281 gcgctgtgcg gaggtgcgcg ccggggcctc gtgcgggagc gggcttcccg acgggcgggg
    17341 ccgcaccgta cactcgcggc tttgcgtgcc gcgtgatcgc ttacgcggcc gtgttgaccc
    17401 ctcggagccc acccccatgc ccaagcagat catcgccaca ccccacgccc ccgccgccat
    17461 cggtacctat tcccaggccg tgcgcgccgg cgacaccgtc tacctgtcgg gccagatcgg
    17521 cctggacccg gccacgatgc agctgatgga cggctttgag gcccagaccg tgcgtgtctt
    17581 cgagaacctg aaggcggtgg ccgaggcggc cggtgcgtcc ctcgccgacg tggtcaagct
    17641 caacctctac ctcaccgatc tggcccattt cgccaaggtg aacgaggtga tggcccgcta
    17701 cttcagcgag cccttccccg cccgcgcggc cgtcggcgtg aaggagctgc cccgcggcgc
    17761 cctcgtcgag gcggacgccg tgctcgtgct gggctgagcc accggtgagc gccccgccgc
    17821 ccgaaacggg ccccgggccg cggggctggc cgggcgtcgg tccgcaactg gccggccgtc
    17881 tggccaggct ggacatcttc gggccacagg acctgatcct ccacctgccc ctgcgctacg
    17941 aggacgagac ccgcgtcacg atgctggccg acgtgcggcc cggctgcgcc gcccagtgcg
    18001 aggtgacggt gctgcactgc gaggtgcaac tgcgtccgcg gcgtcagctc gtggcgcggg
    18061 cggcggatgc cagtggcgag gtgagtctgc gcttcgtgca tttctacccc agccagcaga
    18121 agcagctggc gccgggggcg cgcctgcggg tgttcggcga agtccgtccg ggcttcttcg
    18181 gcgccgagat gatccacccc cgcgtgcgcc ccgtggccga gggcgagtct ttgccggaga
    18241 gcctcacgcc ggtctatccg accaccgccg ggctggccca gtcggccctg cgcaagctca
    18301 tcggtggcgc catgcgccat catccgcccc gcgaggatct gccggacgag gtggtccggc
    18361 gcctcgacct gatggccctg ccggacgccc tgcacctgct ccacgcgccg ccgccgtcgc
    18421 tcgccgcagt ggagctggaa gaccgccacc accccgcctg gcagcgcatc aagctcgacg
    18481 aactcctcgc ccagcagatc tccctgcgcc gcgcctacgc cgcgcggcgt gccaagaacg
    18541 ccccgccgct gccggccgtc gataccctga cccgcgccct gctgacggag ctacccttca
    18601 gcctcaccgg cgcccagcgt cgcgcctggg acgagatcgc ccgcgacctg gcggccgcac
    18661 atcccatgca gcgcctgctc cagggcgatg tgggcagcgg caagacctgc gtggccgcgc
    18721 tggccatgct gcgggcggcg gagaacggtc ggcaggcggt gctcatggcg cccaccgaga
    18781 tcctcgccga gcagcactac cgcaaactcg ccggctggct cgcgcctctc gggctggagg
    18841 tggcctggct gtcgggcagt cgtcgcaagc gcgagcgcga ggccctgatc gctcgcctgg
    18901 cggccggcga actgctgctg gcggtgggca cccacgccct catcgaagac ccggtgagcc
    18961 tgcccggtct ggccctggcg gtggtggacg agcagcaccg cttcggcgtg cgtcagcgcc
    19021 tggcgctgcg cgagaaaggt cgtggcggtg cgccccacat gctgatgatg tccgccacgc
    19081 ccatcccgcg caccctggcc atgagctatt acgctgacct ggatgtctcc gtggtggatg
    19141 agctgccgcc cggccgcacg cccatcgtca ccaagctggt ggccgaggcg cgccgggacg
    19201 aggtgatccg ccgcgtgcac gatgcctgcg tggcggggcg ccaggcctat tgggtgtgtc
    19261 cgctgatcga ggagtcggag gcgctgcagc tcaagaccgc cgaagagagt ttcgccctgc
    19321 tcgccgcggc gctgccggac ctgaaggtcg ggctggtcca cggccgcctc cggaacgacg
    19381 agaaatccgc caccatggcg gcctttgtgg cgggcgagat ccacgtgctg gtggccacca
    19441 cggttatcga ggtgggcgtg gatgtgccca acgccagcct gatggtgatc gagcatgccg
    19501 agcgcttcgg cctggcccag ctgcaccagc tgcgcgggcg ggtcgggcgt ggcgcccatg
    19561 cctcggcgtg catcctgatt tacgcccagc ccctgtccca gaccgggcgg gagcgtctca
    19621 aggccatcta cgaacatgcg gacggcttcg tcatcgcccg cgaagacttg cgcatccgcg
    19681 gtccgggcga gttcatcggt gcccgccaga gcggcgtgcc gctgctgcgc cacgcggacc
    19741 tggaggccga tgcggatctg gtggcggtgg cgcgggacct ggcggaaacc ctgctgcggg
    19801 acgcgccggc ggtggcggat gcgatcaccg cccgctggtt cggcggtcgc gccgcgctgc
    19861 tcaaggcgtg aggacggcgg gccgacgccc gcgtggcggc cattctcggg cgccggcaaa
    19921 cgccctacaa tggcgccgct gccgccgtta tccccaccgc ctgaccccgt gctccgccgc
    19981 tccatgaccc ttgcccactt tccgggccgc gatcccttcc gcatgaccgc cagccgcgct
    20041 ccccgaccct tgcggggctg gctcagcgac gacagttcgc tcaccgcgcg catccgtgca
    20101 cgctgcggca ccttctccct gcacctggtg tcccagggca agggccggcc cttgccggac
    20161 gagctggccg cgctgggtgt gcatcgccac ggtgagctgt ggcagcggga agtgctcctg
    20221 ctggccgacg gggcgccggt ggtgttcgcc cgctccctgg tggccgccag caccgtgccg
    20281 gcggcctggc acctgctccg tcatctgggc ggacggcccc tggccgcggt gctcttcgac
    20341 gacccgcggg tacgtcgctc gcccctggag gcggcccgtc tcgatccccg cgacacccgt
    20401 tggcaccggg ccgccgcggc caccgggcgg agcgatctgc cagccctgtg ggcgcgccgc
    20461 tcggccttcc ggcgggccgg ttcgccgctg ctgatcaccg aagtcttcct gcccgcgatc
    20521 cggagcctgg ccccatgacc tttgccgaac gcctgtccga atacgaaaag ctgatgcgcc
    20581 tcgacaagcc catcggcatc ctgctgctgc tgtggccgac cctgtgggcg ctgtgggtgg
    20641 ccgggggcgg acggccgtcc cctttcatcg tgtggatctt cgtgctcggc acggtgctga
    20701 tgcgctccgc cggctgcgtc atcaacgatt acgccgaccg caatttcgac ggccacgtgg
    20761 aacgcacgcg caaccgcccg ctggcggctg ggcgggtacg tccgggcgag gcgctggtgc
    20821 tggcggcggt gctggcgctg gtgtccttcc tgctcgtgct gcccctcaac acgctgaccc
    20881 tgctgctcag cgtgccggcg gtgtttctgg caggcagcta ccccttcacc aagcgcttcc
    20941 tggcgattcc ccaggcctat ctgggcatcg ccttcggttt cggcatcccg atggcctttg
    21001 ccgcctgcct ggacgcggtg cctgtggagg cctgggtgat gttgctggcc aacgtgttct
    21061 gggcggtggc ctacgacacg gagtacgcca tggtggatcg cccggatgat ctgaagatcg
    21121 gcatcaagac ctcggcgatc accttcggcc gcttcgacgt ggcggccgtg atgctgtgct
    21181 acgccgtgac gctgggcctg ctggcctggg tcggcctcca caccgggcgg ggtgcggcct
    21241 ttctggcggg actggcggtg gcggcgggac tgatgggcta tcacttcacc ctgatccgcg
    21301 ggcgggaccg ggcgaactgc ttcagggcct tcctgcacaa caactgggtg ggtggggcga
    21361 tcttcgccgg ctgggcgctg gactatctgc tcaaacccgt gggctgacgg ctcgcgcgga
    21421 ggacgcccgt caagccgcgc tggggcgcct caacggcgcg gtcgtgcagc ccgcagcagc
    21481 ggcgtgatga ggtcgagggt gcgctccgtg gcgccccggt gggtctgcgc gtaggcctgt
    21541 ccggcggcgc ccatggcgct gcggcgggcg ggatcctgga ggatctgcag cgcgacgccg
    21601 atgccgtcgt ccatgtcgct ggcgcgtact gccgctccgg cttcgatggc ctgctcggcg
    21661 gcggcggaga agttgaaggt gtgggggccc accacgaccg ggcagcccac cgcgcaggcc
    21721 tcgatgaggt tctggccacc caggggcagc cacgagccgc cgatgagggc cacgtccgat
    21781 gcgtggtagt aggcgaacat ctcacccatg gaatcgccca gccagacccg cgtgtccgcc
    21841 agcaccggat cgttgctgga gcggcgttgc aggcccagtc cggccttgcg cacgagcttg
    21901 acgacttcgc cgaagcgttg cggatgtcgc gggaccagca ccagcagtac ttccggcggg
    21961 cacagacggg cgaaggtttc cagcagcagt tcttcttcgc cttcgcgggt gctggcggcc
    22021 aggagcacct gacgcggccc gaagcggttg cggaattcgt ggccgagcgc ctgcatcgtg
    22081 gtcggcggca tgatgtcgaa cttgatgttg cccgtgacct tgagatagcg cgcgcccagg
    22141 gcggacatgc ggttctcgtc ggattcggtc tgggcagcga tcacgctcag gtgggcgaag
    22201 gcttcgcggg cgagggggcc ggcgcggcgg tagccgcggg cggagcgttc agacatgcgc
    22261 gcgttcacga gggccagggg aatatggcgg cggcggcagg tctccaccag gttgggccac
    22321 acctcggttt ccatcaggat gcccatgctg ggacggaagt ggcgcaggaa gcggcgcatg
    22381 acccagggca gatcataggg caggtaagca ctctgaaccc gtggcgcaaa gcgcccgaag
    22441 agctcctcgc cggtggcgcg gcccgtgggc gtcatgtggg tgaggaggag gctgtgatcc
    22501 gggtaggcgt ccagcagcgc ctgaaccagc ggggcgctgg cgcgggtctc gccgaccgag
    22561 accgcatgga tccagatgac gggcagcgac aggcgctggg gatagcggcc caggcgttcc
    22621 ccgaggtgct ggaggtattc cggttgccga cgggcacgcc agaccaggcg cagcacgatg
    22681 aacggtgcag ccagcaacca gagaagggtg tacaggaatc gcaggatcaa gctgcctggc
    22741 ccgatatgcc aacaggcgga agtataaggt cgttacagac cgcacggagc actcctgtgt
    22801 gcgatttacg ctgcaatgca accgtgccag aatgcgggcc tgtcgccggg tcggccggaa
    22861 atgtcggccc gattgttcga attattccgt ccggttatct tcgaccatga aaattctcgt
    22921 caccggcgca gccggtttca tcggcatgca taccagccag ctcctgctgg cgcgcggcga
    22981 tcaggtggtc ggtctcgaca acctcaacga ttactatgag gtcgcgctca agcacaaccg
    23041 tctggcgcgc ctcacgcccc acgagaactt ccgcttcgtg aagatggacg tggccgaccg
    23101 ggacggcatg gccaagctgt tcgccgagga gaagttcgac cgcgtcatcc atctggccgc
    23161 ccaggccggt gtgcgctatt cgctgatcaa tccccatgcc tatgtggaca gcaacctcgt
    23221 cggcttcatg aacatcctcg aaggctgtcg ccacagtcag gtcgagcacc tcgtgtatgc
    23281 cagcagctcc agcgtctacg gcggcaatac ccgcatgccc ttctcggagc atgacagtgt
    23341 ggatcacccg gtgagcatgt acgccgccac caagaaggcc aacgagctga tggcccacac
    23401 ctacagccat ctgttcggcc tgcccacgac cgggctgcgc ttcttcacgg tgtatggccc
    23461 gtgggggcgt ccggacatgg ccctgttcct gttcaccaag gcgattctcg aagggcggcc
    23521 catcgacgtg ttcaaccacg gcaagatgaa gcgtgacttc acctacgtgg acgacatcgt
    23581 gcagggcgtg gtccgcaccc tcgaccgggt ggcagaaccc aatccggcct acgacgccga
    23641 cacccccgat cccggtacca gcaactcgcc cttccgcgtg ttcaatatcg gcaacaacaa
    23701 cccggtggag ctgatgagct tcgtcgaggc catcgagaag gcgctgggca agaccgccga
    23761 aaagaacttc ctgcccctgc aggatggcga cgtgccggcg acctacgcca acaccgacgc
    23821 cctcaacgcg tggaccggct tcgtgccggc gacgcccgtg gacgtgggta tcggtcgttt
    23881 cgtcgagtgg tatcgcagct actacaaggt ctgatgtctg aagcatgccg ggcgacggtt
    23941 cgcccggcgc ggccgaacca cgacgggtgg aaatggaccg tctgaccgtc agcatcgtgc
    24001 tgtaccggcc ggatgcccgg ctgctgcagg cgacgctcga tgcgctcggc gaggcgtgtc
    24061 gggaggccgc gctcacgccc gagcttttcc tcatcgacaa tggcggtggc gccgcgatca
    24121 tggcgtccct gcgctggccc gccgactgga ccgcgcatgt cctcaccggt cacggcaacg
    24181 tcggcttcgg ctgtggccac aacctcagcc tggcgggggc gggggatatc cacctgatcc
    24241 tcaatcccga tctggaaatg gcgccggacg ccctgcgcgc ggcgcgtgat ttcctgcgcg
    24301 ggcacccgga atgcgggctc gtggtccccg cggcgcgctg ggacgatggc cgtatccagt
    24361 atctgtgcaa gcgccggccg gcggtgttcg atctgttcct gcgtggtttt gcgccggcgt
    24421 ggctgcggcg ccgttttgcc gcacggctgg cgcgctatga gatgcaggac gtgatcggtg
    24481 atgccgtggt gtgggatcca cccctcgtca gcggggcctg catgttcttt cgcacggacg
    24541 tcttgcgccg tgtgggcggc ttcgatccgc gattcttcct gtatttcgag gatttcgacc
    24601 tgagcctgcg ggtcgctcgg attacacgga tcgcatatgt gccggcggtc cgggtggtgc
    24661 atcatggcgg tcacgcggcg cgcaagggct ggcatcacgt ccgcctcttc atgcagagcg
    24721 cggcgcgctt ctatggactg cacggatgga catggcgatg aatgatcttg atcagcgccg
    24781 ccgccgcgga ctcaaggcag gtttggcgct gctcgccggc gtcatgctcg ggcgcgcgca
    24841 ggcggcgacg cccccgcccc tgttattggc cgaggtgctg cacggcgatg tggacgtgac
    24901 ccgttactgg gtcagcgaga agcttgatgg cgcccgcgcc atatgggacg gccagaccct
    24961 gcgcttccgc agcggtcgcg aggtgccggc gccggcgtgg ttcattgccg gtctgcctcc
    25021 cgagcccctc gacggcgaac tgtggatggg gcgcgggacc ttcgatgcgc tggcggccgc
    25081 ggtgcgccgc caacccgcgc gggacgatga atggcggcgt gtgcgcctga tggtgttcga
    25141 gcttcccggc ggtgccggca gtttcacgga ccgggcagcc cggatccgcg agatcatcgc
    25201 ccgcgccgat gtgccctggc tgcaggccgt cgcgcagttc cgcgtgccgg accggccggc
    25261 cctgcaggcc cgccttgacg cggtggtacg cgcaggcggt gaagggctga tgctgcaccg
    25321 ggacgacgca ccctacctga ccggccgtca tgacgcgctg ctcaagctca agccccagct
    25381 tgacgccgag gccacggtga tcgcccatct tccgggcaag gggcgcttca gcgggcgcct
    25441 tggcgcgctt gaggtggaga cgccggacgg cgtgcgcttt cgccttggca gcggcttcag
    25501 cgacgccgtg cgccgcgacc cgcccgcgct gggcagccgg gtgacctatc gctaccgaag
    25561 cctgacgccg ggcggcgtgc cgcgttttgc cagtttcctg cgtctgcggg tggagttttg
    25621 agcggacgta tcctcgtcac cggcgggcgc ggcttcatcg gtcggcgcct ggtgaccgcg
    25681 ctgcaggccg acggcgcctg cccgcgggtg ctcacccgcc agacgccggc cgcgccttcc
    25741 gaggtgcagg gcgacgtgac cgacgtgaca gcgctggccc gcgcgtgcga aggggtggag
    25801 acggtgttcc actgtgcagg ccatgcccat gcattcaaga cccatgctgc ggacgagatt
    25861 gcccgtcact gggcggtgaa tttcgagggc agcgtccgtc tgatcgacgc cgcagcggcc
    25921 gcaggtgtgc ggcggctggt gttcctgtcc agcgtcaagg ccgtcgggga gcccggtgac
    25981 gcgcaggccg acgagacgtg gtccgccgca ccggagggtg cctatggcgc agccaaacgg
    26041 gcggccgagg cggccctcct cgaggcgggg ggccggcatg gactccacgt cgtgatcctc
    26101 aggccggtga tggtctacgg cgccggcggg cgcggcaatc tcgagcggat ggcgcgtctc
    26161 gtgcggcgag gatgctttcc gccgctgccg gagacgggca accggcgttc gttgatccac
    26221 gtggatgacg tggtgagcgc gttacgcgtg gtggcgtccg atccgcgggc ggccggccgg
    26281 acctacatcc ttgcccaccc gatcgcgtgt tccgggcggg ccctgtacga tgaactgcgc
    26341 cgggtgatgg ggctcgcgcc gatcgcctgg gcggttccgg cccggctgct gcaggccgcg
    26401 gcgtggctgg cagatgggtg cggcgggctg gcggggctgc acctgccctt caacggcgag
    26461 gttctcgacc gcctcctcgg gtcggcctgc tactcgtccg agcggatccg gatggagctg
    26521 ggctggcagg cccgggttgt gctggcggac ggtttgaagg agatgctcgg tggtcgctga
    26581 ggcagggtac atgctggctg tcgggctggc gtttggcgcg gcctgggggg tgacccggac
    26641 gtgctgccgg cccgattccc tgctgcattt tgtcgaccag cccaacgagc gctcgctgca
    26701 tgtccggccg acgccccgga ccggcggcgt cggcgtgatg gcgggtgtgc tgctgggcgg
    26761 cgggctggcg ttgctggccg ggcacgggtc catggggctc gtgctggcgc tggcgggtgc
    26821 cgtgctcctc gcggtcgtcg gtctggcgga cgacaggctg gatctgtcgg cgcgggtgcg
    26881 tctgctcgtg cacctgggcg tggcggccgg ctttgtggtg atctcggacg caggcggggg
    26941 ggtggaagcc tggcgcgtgc tcccgctggt gctcggcttg gtctggatga ccaatctcta
    27001 taacttcatg gacggatcgg acggactggc cggtggcatg gccgtatggg gcttcgtggc
    27061 ctatgcggtg gcggcaggtg ggcacggcga cggcacgttg gccgccgtgt gcgcctccat
    27121 tgcggcggcg gcggcgggtt tcctgtgctt caactttccg ccggcacgga tcttcatggg
    27181 ggattccggt tcggtaccgc tgggttttct ggccggcgcc ctgggatgcg tgggggtgat
    27241 ggggaaccgt tggccggcgt ggtttccggt gctggttttt tcacccttca tcgtggatgc
    27301 gtcggtgacc ctggcccgcc gggccttgcg tggcgaacgg gtgtggcagg cccaccgcac
    27361 ccactattac cagcggctgg tgcagatggg atacgggcat cgcggtgttg cagtgcgtgc
    27421 gtatggtttg atggcagctt gcgggggcag cgcgttgctt gtcatggata ggggtgttgc
    27481 actgcaatgc tttgttatta caatgtggac attcgtctat atggtcgtgg ggttccggat
    27541 cgatcgcgcc tggcgccgtt ccggatgacc cgtctgcgat gcgctcactc cagaaattcg
    27601 cccacccgct tctggtattt ctgtcggacg ctctgggtat ttgtctcgcc tggttcgtcg
    27661 cctacctgat ccgcttcaat ctggccatcc cggccgagtt catgccggcg atgctgttcg
    27721 gtctggccgt ggccttgccc atccagtgcc tgttcctgcg ggtgttcggg ttgtatcgcg
    27781 ggatctgggt cttcgccagc ctgcccgatt tgcaccgcat cgggcgtgcg gtggccgccg
    27841 gcgctgtcgt ggtgcccctg gtgctggtgg tgagcgtgcg gaccgaaccg tggattccgc
    27901 ggctggtctt cctgctgcag ccggtgctgc tgctcctgta catggggggc acccgggcgc
    27961 tgtaccggag ttggaaggag ttccgcaact ttggtgcgct gcgcgcccag gggcagtcgg
    28021 tgctgatcct cggcgcgggg gtggccggcg cccatctggt gagggagctg gcggcctcgg
    28081 cccagtggcg ggtcgtgggc ctgctggatg atgaccccca caaacagcgg cgcgagatcc
    28141 acggctacaa ggtgctcggc cccatctccg agctcgaacg ctgggccgaa accctgaagg
    28201 tcggccatgc catcatcgcg atgccctcgg tgcgccatga ggtgcggcgc gcggttgcca
    28261 gcgaatgcgt ccgggtcggg gtgaccgccc tgatcgttcc gggtgtcgac gaacggctct
    28321 caacgtccca cagcctcagt gaggtgcgtc agctcgacgt ggaggatctc cttgggcggg
    28381 atcccgtgcg catcgacacc gaacaggtgc aggattatct gcggggcaag gcggtcatgg
    28441 tcacgggcgc gggcggatcg atcggcgcgg agctgtgccg gcagatcgcg cgcttcgagc
    28501 cgtccgtgct tgtctgcttc gaactcagcg aattcgccct gtaccgtttg atcgaggact
    28561 tcgcccggga gtttccgcgt ctgcagctgg tggcgctggc cggggacgtg aaggatgcgg
    28621 tgcgggtgga cgaagtcatc ggccgctatc gtccgaacgt gatcttccat gcggcggcat
    28681 acaagcacgt gcccctgatg gaagaggaca acgcctggca ggccgtgcgc aacaacgtga
    28741 tgggcacctt ccaggtggcg cgtgcggcgg tggctttcgg tgtgccgcgt ttcgtgctgg
    28801 tgtccaccga caaggcggtc aatcccacca atgtgatggg cgccaccaag cgactggccg
    28861 agcaggtgtg tcaggccttt gctctggagg ggcgttccca gttcgagatc gtgcgctttg
    28921 gcaatgtgct ggccagtgcc ggcagcgtga tccccaagtt ccaggcgcag attgccgccg
    28981 gcggaccggt gaccgtgacg catcccgaga tcacgcggta cttcatgtcc atccatgagg
    29041 ccgcacagct ggtgctgcag gcagggtcca tggggcaggg gggcgagatc ttcgtgatgg
    29101 acatgggaga gcccgtgcgc atccaggacc tggcgcggga catggtccgc ctgtccggtc
    29161 ggtctgagga cgacatccgc atcgtgttca ccggcttgcg tccgggcgag aagctctacg
    29221 aagaggtgct cgccgattcc gagcggaccc gcccgaccca tcatcccaag ctgcgcatcg
    29281 cccgcgccca ggctgtcgag tccggctggc tcgacatgct ggcattgtgg ctgcggcagc
    29341 gtcgggccgt gccggcggca gaggtccggc gcgagctgcg gcgctgggtg ccggaataca
    29401 caccgtcgag tcacgccgtc ccgctgacga aggtgtctga caccgggcgt cagcgcgcct
    29461 gaggagcgtc catggccacc tacgcgatcg gtgacatcca gggctgcttt tcagccctgc
    29521 aggcgctgct taagcgggtg gagttcgatg cgggccacga ccgcctgtgg ctggtcggcg
    29581 atctggtgaa ccgggggccg gattccctca agaccttgcg cttcgtgcgc gatctggggc
    29641 cggccgcagt gaccgtgctg ggtaatcatg atctctacct gctgatggtg gcctacggga
    29701 cggtgcgcag ccggggcaag gacgacacga tccaggcggt tctggacgcg ccggaccggg
    29761 aggcgctgct gggctggctg cggacacgcc cgctgatgca cgtggagaac ggctttgcca
    29821 tggtccacgc cgggctgctg ccgggctgga cggtgagtca ggcccgcgct ctggcgcggg
    29881 aggtggaggg tgcgctggcc gggccctatc acgccgatct gctccacaac atgtggggca
    29941 gcgagcccgc cgcctggcgc gacgatctgc gggactacga gcgcatgcgg gtgatcgtga
    30001 acgcgatgac gcgcatgcgt ttctgcacgc cggaaggcgt gatggacttc aaggtcaagg
    30061 gcgaggtggc acgggcgccg aagggatatc tgccgtggtt cgatgcgccc gcccggcgca
    30121 gcaccgatgc gacggtggtg tttggccact ggtcggcgct ggggctcaag atccggccga
    30181 atctgatggc cctggattcc ggctgtctgt gggggcgcga gctgacggcc gtgcggctgg
    30241 aggaccgtca ggtctttcag gtgtcctgtc ccggactcag ccacggcaag ccgcagccgt
    30301 cctgattcag tcgatggcga tgcagctgac gatggcttcc cgggcactgc gggcgagttg
    30361 cttgcggtcc ggcgtctcgc cggtggacag gggggccagc aagcgcaccc gcgccacgat
    30421 tgtccgttcg gcgacgatgt tcaacagggt atccacgagg ctcaggtcgc catcataggc
    30481 gggcgcgcgc gtgtaccggc cggtggcgtc gcggtaggtg agtgcgacgg gctggagcgg
    30541 cgcaccgacc tggatggcgg gttgcagcag ggcgccgtga aagccgagga cgccccggcc
    30601 atcggtggtc gtgccttccg gaaagatcgt cacatggcgc ccgcgggcaa gcagcgtggc
    30661 gatctcgccg ttcacgatgc gtgcgtggcc gcgactgccg cgccgcagga agaccgtctc
    30721 gttgcgggcg gcgaaccagc cgatgaccgg ccacgcgcgg acctctgcct ttgaaatgaa
    30781 ggtcgacgga caggtcgcgt tgatcacgaa gacgtccagc caggacacat ggttggcgat
    30841 cagcacgccg ccgggtgcga cgggaaggcc gcccacgtca aggcgcacgc ccagcgtccg
    30901 caacagcccg cgggaccagg cagcccggat ggacgccctt cgcggtgacg cgaggacggg
    30961 aaacagcgct gcggtgacga gcagaccgcc tgtcagccag aaggcgaggc cgacgaggcg
    31021 ggcgaggcgg agcgggcggg gtgcgggttt cagtccttgc gtcccatgaa gtggcgggcg
    31081 taacgggcgt cgagccgcga gatgggcagg agcacgggca gatcggcggt gttgaagtcg
    31141 ggatcccagg ccggctcgcc gcagatccac gcgccggcgc gcaggtaacc cttgatgagg
    31201 gggggtgtct cggcgtccag atcctggcgc agggcgccga tgggcagcgg agtgcgcggg
    31261 aacacgcggt attccagcgg gcacaggtgg ctgtccttga gccgattata caggctggcc
    31321 gcggcgtgac cgccgtccgc catgctgatg gaggcgcagc cgatcaggta gtcgtggccg
    31381 ttttcgacca tgtagcgggc cagcccggcc cacagcaagg tgatgacggc accggagcgg
    31441 tagtccggat cgatgcagga gcggccgacc tccaccatgc gctcgcgcag gtgacgcagg
    31501 cgggtgaggt cgaattcgct ctcggagtag tagctgccca cttcccgggc ggcgctgggg
    31561 gacaggatgc ggtaggtgcc gacgatgcgg ccggcgtcct cgtcacgtac gatgaggtgg
    31621 tcgcagaagg cgtcgaacag gtcgcgatcc acgcccggga tacgggtttg caggcgcgcg
    31681 cccatttcgt ccgcgaagac gcgataacgc agtttctggg cttccaggac ttcggtctgg
    31741 ctggcggcca gcgtcacctg caggttgcgg ccagggcgat gggcgacgtg ttgttccttg
    31801 tgcagcatgg cggacaatcc tcaaatgggg ttgccggcat tgtgtaaagg ctgcgttgcg
    31861 tcgctgtgac gtggtcgtga cgggcgtgtg acgaagacgg ggtcggaaaa cgcaaagggg
    31921 cgcggaagcg cccctctcgc ggtggagcgg cgttccgcgt cagcgtgtca cgcgggtggt
    31981 gcggccgtcc tgcagcaggc gcacgcgctc accgggcttg aagccctcgc cctcgtcttc
    32041 ctggacgatg gcaagcattt caccgttgtc catcttcagg gtgatttcca gaccgttctt
    32101 gcgggtcagg ccttcttcgg ctgccgagcc ggccagaccg cccaccacgg caccgaccac
    32161 ggcaccgatg ctggagccct tgcctccgcc gacggtgctg ccggcgatgc cgccaacggc
    32221 ggcgccgccc agagtgccga tcggcgactt ggtgccttcg agtttgacga cgcggacgct
    32281 ttcgatgacg cccatgcgga cattcatgac gcggcgggct tcggcacgct cataggtgcc
    32341 gccgcccagt ccggaggcac agccggtgac ggatacgacg gccacgaggg cgagggagag
    32401 ggggagcaga cgcatggttt ttcctttcac cacagggttg tgccgaaggc ttcgcgaaag
    32461 cgttcggcga tttcgtcgcg gcggggggtg tggttctggc ccccgcggct ggcaatcttg
    32521 agggccccca tcagcgacgc aaggcggccg gtgagcgccc agtccaggcc cgcctcgatg
    32581 ccgtagagca ggccggcacg gtaggcgtcg ccgcagccgg tggggtcttc gacggccgcc
    32641 ggccggacgc agggaatgtc gatgcgttgc ccgccggtgt ggatctcgga gccttcgccg
    32701 ccgcgtgtga cgatcagtgc cttgagttct ccggacaggc tggccggcgt ccggccggtg
    32761 cgctcgcaca tcaggcgcgc ctcgtagtcg ttgaccgtgc agtagtcggc cagttgcaga
    32821 caggcgacga gctcttcgcc ggagaacatg ggcaggccct gaccgggatc gaagatgaac
    32881 gggatgccgg cttcggcaaa ctggcggcag tgttcgagca tgccatcgcg gccgtccggg
    32941 gcgacgatgc ccaggcgcac gtcgcgggca tcgctcacct tgttgaggtg ggactgggtc
    33001 atggcacccg gatggaaggc agtgatctgg ttgtcatcca gatcggtggt gatgaaggcc
    33061 tgtgcggtat aggtgccggg cacgtgggtg acgtgggcac gggacaggcc gagaccgtcg
    33121 agacgctcca ggtacggacc ggcgtcatcg ccgacggtgg ccatgatcag cggatcgccg
    33181 cccaggagct tgaggttgta ggcgatgttg cccgcgcagc caccgaactc ccgccgcatc
    33241 tccggcacca ggaaggagac gttgaggatg tggatctgct cggggaggat gtggttgcgg
    33301 aagcgatcat ggaacaccat gatcgaatcg aaggcgatgg agccgcagat gagggtggac
    33361 atgggcgtct tgaagatgtc gttcggatta tagaagccac gcatggatgc cgggatggca
    33421 tgtgttgcgg gatgtttcgg cgcgctcagg ggtggaaggc gaggacctga tagcctgctg
    33481 cggccacgcc gggggcgtca aagacgatgc gggcggagat ttcctggttg ggcgcgaagg
    33541 ccgccttgcg ggcatcgggg ggcagccatt gggccggttc gagcacgcgg cggacgaggg
    33601 ccttatcgcg ggcgtccgtg agggtgatct cgaggtaggg gtaggcctgc tcgaaatctg
    33661 cccggttgcg caaggtggcg tagagggtga accgcgcttc ccggctgccg tccggctgca
    33721 tgtcggaact ttcgatggtg atcaggctgg aatcccgcgg caggggcatg tcgcagccga
    33781 tccgatcgca cagcgcttcg agggccgggc gcagggcggg tgcggcgagg gcgatccggt
    33841 tgcggaatac gagcaccccc tgcgcgagca gcgcgacgat caggaaggtg acggcggcgg
    33901 cccagacggc ctggcgcaga ccgcggggtg gggcgtcctc gtcatccgct gcggaggtgt
    33961 cggggccggc ctgcggagca ggcaggacga agggagcgtc gtctgcgtcg gccaccagcg
    34021 tgaacggctt gggcgcgggg tctggtgctg cgccaggttc gggttcgagg cgcacgtcgt
    34081 tcggaggggc gccaaagtcg ggcggcagcg ggaaggccgc ggcgggcggc ggctctgtgc
    34141 ttgcgccatc gtcgcgcgct gactcgatcg gggcgggctc ggtcgcgcgg gcaatctctg
    34201 cgccgcgctc atccacgtcc aacgggagaa ggtgctcgtc gggcggcccg tcaccgtgtc
    34261 gcgccggtgg gtcgtcaagg atgaaggcgg gctcgacagg gggcgcgtcg tccaggcgct
    34321 cgggcagggt gtgcgtgctt ccgtcgggaa acggatctgc aagcggtgct gcgtcgaagg
    34381 ccggcgtctg ggatgtgggc tcgtcagcag cgtcaagcag ctcgggctcg gcaggtgcgt
    34441 cgtccggcag gggcggaatc tcgccgatgg gctcgatatg gggcgacggg ctgtcagggt
    34501 ccggggctgt ccagtcgtgc cgagccctga cggatttctc ctcgaggatg aagaccggac
    34561 tttcaaccgc ttcggcgagg ggttcgccat cgacgctggc gggcggcggc gggaccgggt
    34621 tctcgacgag ggtttcgagg gcgttgaagg cgtgctggca gtggccgcag cgcacgcggc
    34681 cctggcgggc gcgcagttgt tccggacgaa cccggaaggt cgtggcgcac gcagggcagc
    34741 gcgcgaggat catggcccgg tctgctccgg gcggacgcct tcgagccgca cccagccgtc
    34801 cgacaggtcc gccacgcgta atgcgatgta gggcgcgtag gctgcgatga cctggtcggc
    34861 ctgggcttcc agcacgccgg acagggcgat ccggccgcca ggggcgatgt ggctggccag
    34921 aagcggtgcc agcatcttga ggggattggt gaggatgttg gcgaccacgc ggtcgaactg
    34981 gccgtccaga gcggtggacg aatgcaggag ggtgaggtgg acgccgttgt tgcgggcgtt
    35041 gtcagcggcg gtctcgacag ccttctcgtc gatgtccaca ccggttaccg tgccggcgcc
    35101 gagacgtgca gcggcgatgc cgaggatgcc tgagccgcag ccgtagtcga ggacgctctg
    35161 gccgggctgc agactcgtcg ccagccattc caggcacagg cgcgtggtcg gatgggagcc
    35221 ggtgccgaat gccatgccgg gatcgaggac gaggttgatg gcgtcgggat cgggcgcggc
    35281 gtgccacgag gggacgatcc acagtcggtc ggtgacgcgg atcgggtcga actggctctg
    35341 ggtgagctgg acccagttct gctcctcgac ttcctcgatg tggaaggggg gcacggcgtc
    35401 gaggccggcc tcaccggctg cggccgacag ggcgctggcc aggtcggcgt ccgggtcgaa
    35461 gagggcgacg acgcggctgt ggtcccacag gctggccgga tgcatgccgg gctcgccgaa
    35521 ctggggtcgc tcggcctcgg tgccggcgtc ggcgtcgtcg atgctcaccg acagggcgcc
    35581 gtgctccatg agggcttcag agagggtctc ggcgcgggcc gcgtcggcct gcaggatgac
    35641 ggagagccac atggtgtcag ccctgtttga cctcgttgcg ggcggccagc ttgtgctcca
    35701 ggtagtggat gctggtgcca ccctcgatga acttggcgtc acgcatcagg tcctggtgca
    35761 gggcgacgtt ggtcttgatg ccttccacca tcatctcgga gagggcgatg cgcatgcgcc
    35821 ggatggcctg gtcgcgggtg tcgccgtagg cgatcagctt gccgatcatg gaatcgtagt
    35881 gctggggtac cgtgtagccg ttgtacacgt gcgaatccac gcggatgcca ggaccgccgg
    35941 gcacgtgcca gtgggtgatg cggccggggc agggggtgaa cttgaagggg tcctcggcgt
    36001 tgatgcggca ctcgatggcg tggccgcgca ggacgatgtc cttctggcgg tagcgcagct
    36061 tctggccggc ggcgacgagg atctgttcct ggacgatgtc cacgccggtg atgagttcgg
    36121 tgaccgggtg ttccacctgg acccgggtgt tcatctcgat gaagtagaac tcgccgttct
    36181 cgtagaggaa ttcgaaggtg ccggcacccc ggtagccgat cttgcggcag gcctcggcac
    36241 agcgttcgcc gatgcgggtg atgtgacggc gctcgatgcc cggcgcgggg gcttcctcga
    36301 tgaccttctg gtggcgacgc tgcatggagc agtcccgctc gcccaggtag atggcgttgc
    36361 cgtgctggtc ggcgaggacc tggatttcca cgtggcgagg gttttccagg aatttttcca
    36421 tgtacaccac gggattgccg aaggccgcct gggcttcggc gcgggtggtg gtgacggcgt
    36481 tgaggagggc ggcttcggtg tgcaccacgc gcatgccgcg cccgccgccg ccgccggcgg
    36541 ccttgatgat caccgggtag ccgatgctgc gggcgatctt gacgatctcc ttgggatcct
    36601 ccgggagggc gccttccgag ccgggcacgc agggtacgcc ggccgccttc atggcgtcct
    36661 tggcggaaac cttgtcgccc atcaggcgga tggtttcggc gcgggggccg atgaagacga
    36721 agccggactg ctcgacccgc tcggcgaagt cggcattttc ggagaggaag ccgtagccgg
    36781 ggtggatggc ttccgcgtcg gtgacctcgg cggcagagat cagggccggg atgttgagat
    36841 agctgagggc ggacgaggcc gggccgatgc acacggattc gtcggccagc cgcacgtact
    36901 tggcatccgc atccggctcg gagtgcacgg cgacggtctt gatgcccagc tcgcggcagg
    36961 cgcgcagcac ccgcagggca atctcgccgc ggttggcgat gaggatcttt tcgaacatgg
    37021 ccatggcgat cagccgatga tgaagagggg ttcgccgtat tcgacgggtt cgccgttctc
    37081 gacaaggatg gccttcacga cgccggccac atccgcctcg atctcgttca tcagcttcat
    37141 cgcctcgatg atgcacaggg cttcgccggc cgcaatgctc tggccgactt cgacgatggg
    37201 cttggcgccc ggcgcgctgg cccggtagaa ggtgccgacc atcggggact tgaccacgtg
    37261 gccatcgggc agggccggtt ctgcggcggg cgctgccgcg gcgggggccg ctgctggcgc
    37321 gggcacattg atggccatcg gggccggcgc cgcgtaggcc actggcgccg ggccggcgct
    37381 gtgtttggcg atgcggacct tttcctcgcc ttcagtgact tcgagttcgg aaatgcccga
    37441 ttcctggacc aggtcgatca atttcttgag cttgcgaaga tccatagtgt tccccttgaa
    37501 tgatgacaac ccgccgtccg gcgggccgtg atgtcgattt caggcgcctg gcgcggtgcg
    37561 caggcgggcg aggacgaatt ccagggcgag ctggtagccc tgggcgccca gtccgcaaat
    37621 gacgcccacc gccttgtccg agaaatagga gtgatggcgg aagggctcgc gggcgtggat
    37681 gttggacagg tgcacctcga cgaagggaat cgcgaccccc gtgagggcat cccgcagggc
    37741 gacactggtg tgcgtgtagc ccgccgggtt gatgatgatg aacgcgacgc cttcgctttc
    37801 ggcagcctgg acccggtcga tcagttcgcc ttcgtggttg ctctggaagg attccagaca
    37861 cacgccgtcg gcgcgggcac ggctttccat ggcgccatgg atgtccgcca gggtggtgag
    37921 accatagatg ccgggctcac gccggcccag caggttgaga ttggggccgt ggaggacaag
    37981 gattcgggtt tgcggaagct cgccgacggt gccttcctga gtgcgatttt ttcgcgtcat
    38041 ggcggcaagt ttgccgcaat tcggcgcacg ttgtccacct cggcagcgcc agccgggtgc
    38101 gtcagctgcc gctcagggcg gccagttgcc ggtccagttc gtcctgcggg aatgcgccca
    38161 gcttgacgcg ggcgacgcga ccttcccggt cgatgatggc cgtgaagggc agtccctggg
    38221 ccgcgttgcc gagggcttcg gtgagggtga tgacgtccgg cgaggcgatc aggagcgggt
    38281 agggaacggg tgatttggca gcgaattcgg cgacgttctt gggactgtcg atgctgatgc
    38341 ccacgaactg gaccttgcgc cccttgtact tgtcgctgag ggtcgcaaat tccggcatct
    38401 ccttgcggca gggggggcac caggtggccc agaagttcac caccagcacc tgaccacgcc
    38461 attgttgcag ggcctgttgc gtgccggcgc tgtcgggcag cgagagcgcc atcaggcgtg
    38521 ctgtggcatc gcgggcagcg gcggtcggcg ctggtgacgg cgggtcggtg acgtgctggc
    38581 gggtgaggag gccggcggcg gctgcaagca ggccgagggc ggcaattgcg ctccaggtgg
    38641 tcaggcgagt catggcgtcg aggtctgttc cgtttgattg accagggctt cgacggtggc
    38701 gagccgggcg cgttccgtcg ggcgcgtgcc atggccgcgg cgtccgaggc gttccacgtc
    38761 ctggggatag acccgcaggg tgaccggaat gtcgtcccag tcgagatgca ggatggcctc
    38821 ggggccgtcc ggctggttgc ggcggggctc gcggtggtcg taggggacgc cctggttgag
    38881 gaggaagatg tccaggtcct tggcgctttc cgggaagagg tcgatttcca cctcggcata
    38941 gcggcctgcg gtgccttcga ggacggcgcc ggtgagatag gggcggaagg gctccagttc
    39001 gcgcatcacg tcgagggcgg cgcggcgcat ggcgagctgg cgttccaggt gttcctcgtc
    39061 ctggaagacg gcgaggtagg tgcggacctc ggcttcgatt tcggcgttgt tggggagttc
    39121 gtcggcggcg aaggcgccaa gctggcgggc cgccttgcgt ttggcgaaac cgtagtcgct
    39181 gatgccgtct tcggcaatca tgcgggcggc gagggctgcg atctcgcggc gcaggtgctt
    39241 gcggtgggga acggcgggac gcggcatggg cagacggtgc actccgttcg attacaatgc
    39301 cggccattct acacacgact gttgcggcgc tgtttccgcg acgaagcgac catgcacatt
    39361 cacattctgg gcatctgcgg gaccttcatg gggggcgttg ccctgctggc ccgggcggcc
    39421 ggtcataccg tgacggggtg cgatgccaac gtctatccgc ccatgagtac ccaactggag
    39481 gagcagggca tcgggctgac cgaaggctat ggcgtcgagc agctcgacct gaagcccgac
    39541 gtcttcgtgg tgggcaatgc gatctcgcgc ggtaacccgc tgctggaggc gatcctcgat
    39601 gccggtctgc cctacgtgtc ggggccccag tggctggcgg agcacgtcct tcaggggcgc
    39661 tgggtgctgg cggtggcggg cacccacggc aagaccacga ccacgtccct gctggcgtgg
    39721 attctccagg acgcggggct gaatcccggc ttcctggtgg gcggtgtgcc gaagaacttt
    39781 ggcgtgtcgg cacgcttgac cgaatcgccc ttcttcgtca tcgaggccga tgagtacgac
    39841 acggcgttct gcgacaagcg ctccaagttc gttcattacc gcccgcggac ggcgatcctg
    39901 aacaatctgg agttcgatca tgcagacatc tttgccgatc tggcggcgat cgagacgcag
    39961 ttccaccatc tggtgcggat catgccgcgt gccgggcgta tcgtcgccaa cgctgccgaa
    40021 gagaccctgc agcgggtgat cggccgcggc tgctggtccg aactggaatg gttcaacgat
    40081 gcggcgggct ggtcggtggc ggaaggggcg gtggaggatg aggtggtggt gcgtcatctg
    40141 ggccgggaga tcggtcgccg gcgcctgccc ctgtccggtc ggcacaaccg ttcgaatgcc
    40201 ctggcggccc tcgccgccgc gcgtcatgtg ggcgtgacgc cggaaatcgc cctggacgca
    40261 ctgacgcgct tcgagggcgt caagcggcgt ctggaattac gcggcactgt gcgcggggtg
    40321 gcggtgtacg acgatttcgc ccatcacccg accgccatcg ccctgacagt gggcggcctg
    40381 cgccgtcagg tcggggcggc gcgcattctg gcggtgctgg agccccgctc caacacgatg
    40441 aagctgggca ccatgaagga tcagctgccg gccagcctga ccgacgcgga caaggtcttc
    40501 tgctacgccg cccacctggg ctgggatgcc gcggcggcgc tggcgccgat tgccgacaag
    40561 tccctcgtga cggacgattt cgacgcgctg atcgcggcca tcgccgccga ggctcgggac
    40621 ggcgaccgca tcctggtgat gagcaacggc ggcttcggcg gcatccacgg caagctgctc
    40681 gacgccctcg cccgctgagg ggccgtccgc cccggtcagc gcagcggttg gggcggcggt
    40741 gcgaagaggc ctgtggtgcg ggcagggcgg gccgtctgca ctgcgccctg cgtgaacagg
    40801 cgcagcgtcg ccgccagcgt ggcgcacagg agcagggccg cgcctgtgtt gtgggcgacg
    40861 gccaagggca gaggccaatg cagcaggacg ttgccgatgc ccagcgcgag ctgcaagctg
    40921 agggccacca gcacggcaag cccttcgccc cgccagccga cgcgacccat cagggcgacg
    40981 cccagggtcc cgacgaggcc cagcacgacc ccggcgaaga gccggtgagc ccagtggatt
    41041 gcagtcaggg cggtaccggg cagcagggcg ccgcttgccg ttgcccccag ttcccggtcc
    41101 agggtaaagg cgtggtgggt gtccagttcg ggccaccagc ggccctggca ggtggggaaa
    41161 tccgggcagg ccgccgccgc atagttgctg ctcacccagc cacccagggc gatctggacg
    41221 aagactgccg ccagcgcagc aagcgcgagg ctgcgcagcc acgcgggggc gggacggcgg
    41281 gacggggcgg ggcgttcccg cagccacagc accgccagca gcgccagggt gctcaggcca
    41341 cccagcaggt ggccggtgac gatcaccggc ttgaggagtc gcgtgaccgt gagcatgccc
    41401 agcaggccct gggcgcagac gacggcaagc aacgcgattt ccagcccggg cgtccgttcg
    41461 gcgcgtcggc gccaggccat ggcgaccagc gcgaggatgg ccagccctag accgcttgcc
    41521 gcgtagcgat ggatcatctc cttccaggcc ttgggggctt ccacgggtct gtcgggaaag
    41581 gcgttgcggg cggcggccag atcgggggcc tgttccggca cgacgagctg gccgtagcag
    41641 ccgggccagt ccgggcagcc cagaccggca tccgacaggc gcacgtaagc gcccagggag
    41701 actacgacca gcgccagcgc cagcgcgcac tccagcacga ggcggttgat gcggctcaca
    41761 gcaggcggtc caggaagagg gcggcgaaca ggcctgtgag atagatcagc gagtagcgga
    41821 aggcgccgcg ggcgcgatcg tccgagtagt cgcgccagag ccgccaggcc tggtgcagat
    41881 accccaggtt gaggccgctg acgagcaggg cgtaggtgcc gccggacagg cccagggtgc
    41941 agggcagata ggacacggcg gccagtagta cggtgtagag caggatgtgc aggcaggtga
    42001 gcggcacgcc gtggctcacc ggcagcatgg gcaggccggc gcgggcgtat tcctcccgcc
    42061 ggtagcaggc caaggcccag aagtggggcg gcgtccacag gaagatgatg aggaacaggg
    42121 cgagggccgg caggccgatg gtgccggtca tcgcagccca gccgaggacg ggcggcatgg
    42181 cgccggacgc gccgccgatg acgatgttca tgggcgtcgc gggtttgagg agcagggtgt
    42241 agacgatggc gtagcccagg aaggtggcga gggtcagcca catggtcagg ggattgaccg
    42301 ccatgtgcag tacccacagc ccggcgcccc cggtcaggca ggcgaggccg agggtctccg
    42361 caggggtgat caggccgagg ggcaggggac gggcccgggt gcgggccatg cgtgcgtcga
    42421 tggtgcgttc caccaggcaa ttgatggccg cggcggcccc cgccacgagg gcgatgccca
    42481 gagtggcagc cagcaggcgg ccgaggggcg gccagccggg cacggcaagg aacatgccga
    42541 tcagtgcggt gaacacgatc aggctgttca cccggggctt gcactgggtg acaaaggcgg
    42601 ccaggcgcag cgtcaggggg gcgcggaggg cggtgggggc gtgcaggagg gcaggcatag
    42661 ggtctccggg gtgagcgtca gccggcccac gcgtagcgga gcaggcgttc catgtccttc
    42721 agcaggccgc gggggtctgc gtcggcggcg tagcgcagga tgaggcggcc ctgcgggtct
    42781 acgacaaaga cctgcgggcc ggcgcgatcc aggtcgaagc gctggcgggc ggcttcgctg
    42841 gctgcgatgg cagtgtgcag gtcgggctgc agggtgagga gcggggtggc gtccggatga
    42901 ggctggtctg tgagccagac acgctggacg cggttcatct gcttgtagag ggctacatgt
    42961 acctggcggg tggcgacgat ccattgccgg caggcgtcct cgcagggacc ggcggatgcg
    43021 accagcagag tccagtggtc cgtaaaggtt gcagggtcca gcgggcgacc gtccgcgcca
    43081 aggagttcgg gcagcgtgcg cgcggggttc agcaggtcgc cattggccgc cggggcggca
    43141 ggtcgccagc cgtaggcgaa caggccgccg ccgatggcga ggggcagcag gaacaggccg
    43201 aggatcagcc acagggggcg gtagcgggcg gtcaccgacg cctccatgcg agggcgaacc
    43261 agatcaggcc cgcggcggcg ccaaagccgt accactggta ggcgtaggac aggtggcgct
    43321 cgtaacgctc gtcggcgcgg ggccattcgc gggcaaagcc ggccggcgcc gacgggtcga
    43381 gctggacgat gacgggctgc accggaacgc cggcgaggcg ggcgaaacgc tccagatcca
    43441 ggttctgcca gaccgcgggc catccggcgc tggcagcttc ggtgccgagg gtgaagaaac
    43501 ggtggcccgg gaccatcgcg acgccgtcga gtgtcaccgg gccggtgggc gtgtccacct
    43561 gggggacatc gctatggcgg gccagggcgg ggatccagcc ccggttcacg aggatgcgcc
    43621 ggtcgctgcc ttcgaggcgc aggggggtga tcacgtggta acccgcctgt tcccgatgga
    43681 cgcggttgtc cacgaagaac tggtactcgg tttcgaagcg gccccgcagg cggacgggac
    43741 ggccgtggag gacctcggcg tccacgggcg cggcggtgat atcgacggcg gcgtcgtggc
    43801 cgaggcggtc gaggcgggcc tgggcggcct gtttctgatc tgccttgccg gcctgccagt
    43861 gaccgagttg tacacagagc gtcgccacgg ccagggccac gacgccgccc gccagggacg
    43921 gccggaagcg gcggccgctt gtggcggcca gcaggccggc gcacaatcgc cgcaccccat
    43981 tcgaggagcg ggcagtggtc ttcaagttgg cagtggtgct catgctggtg gcggtgatcg
    44041 tcagcctgtt ttccggcctg ttcttcctct atcgggacaa aggggacggc gcccgcgtgg
    44101 tgcgtgccct gacgatccgc atctccctgt ccgttctgct ctttctggcc ttgctggtgg
    44161 cgtaccggtt tgggctttac agccagtaga cgacgacgaa gagcagcagc cagaccacat
    44221 ccacgaagtg ccagtaccac gccaccgcct cgaaggcgaa gtggcgctgg gcgtcgaaat
    44281 gaccggccag acagcggccg gaaaccaccg cgagcatgat ggcgccgagg gtgacgtgga
    44341 atccgtggaa gcccgtgagc atgaagaagg tggcgccata tacgccggct cccatcgtca
    44401 ggcccatctc ggtgtaggcg tggtggtatt cgtaggcctg gaagccaagg aagatcaccc
    44461 ccagcagcac ggtgaggatc aaccccaggt tgagctgggc acgcttgccc ttcatcaggc
    44521 cccagtgggc ccaggtgagg gtcatgcccg aggtgaggag cagggcggtg ttgatggccg
    44581 ggatgcccca ggcgcccatg ggcgtgaagc tggcgccctt ggggccggcg gtcggccagc
    44641 cgccggtgta gccctcgtag agcgtgaaca tcggatccat gcccgccagc gccggcacgg
    44701 agatggtgcg acagtagaac agggcgccga agaaggcggc gaagaacatc acctcggagc
    44761 cgatgaacca caccatgccc tggcggaagg acttgtcttc ccagtcggtg taggcgccgc
    44821 cctgattttc ggcaatgacg gcaccgaacc agcggaacag caccccgatg atgaccatcg
    44881 cgccgccacc catgacccac ggacccggcg cgaagcggtt catgagcagg atgaatccca
    44941 gggcgagcag gaacatgccg cccgacagga tgaagggata gacgctgggc tgcgggacgt
    45001 agtagtgacc ggtgtgaggc gtggaggcgt gagaggcgtt caaggcgggc tccctgattg
    45061 tttagcgggg cgtgggggcg ggaaagaagg cgtaggccag ggtcagttcg cggaggtccg
    45121 ggccgatgtc gcgatccacg atgaaggtca acgccatctc gcggctttcg ccgggggcga
    45181 gggtctgttg cttgaagcag aagcaatcca gcttgcggaa gtgggacgcc gccaccgtgg
    45241 gtgacacgct gggtaccgcc tggccgacga tgggctgatc ggacaggttg cgcaccagga
    45301 agcgggtggt gcgcagttcg cccggatgca ggtcgaaggc gggttccagc gggcggatgt
    45361 cccagggcag gccgggcatc accgtggcgg tgaattgcac attgaggctg cggctgctat
    45421 ccaccgcagt ggcgccggcc tcctgggcgg agagggcgtc cttgagggtc ttgccgttga
    45481 aaccgacggc cttgcacagg gtgtcgtaga ggggcaccag ggcaaagccg aaaatgaagc
    45541 agcctcccgc cagcagcacc agacggccga ccaggcggcc gtggcggggc gaagtgggca
    45601 ggctggcatc gtgcatcgcg gtcatcggga tctcagcgct ggacgaagaa gccgagggtg
    45661 tagagggcca ctgcggcggc acccgcccac caggcgaggc gggcagaggc gcggcggcga
    45721 cgggccagat cggcgtcgga gatcatctgc atggggtggc tcctcagcgg ctctcgatga
    45781 cgggctgggt ttcccaggag tggtagggcg ggggcgagga cagttcccac tccaggccat
    45841 cggtgccttc ccagacctgg tcgctggcgg gttcgccgcc cttcacgcag cgccacacgt
    45901 tgtaggcgaa gatcagctgg gacaggccca ggcagaaggc gccgacagtg ctgatggcgt
    45961 tgaactcggc aaactgcagg gcgtagtccg ggatgcgccg gggcatgccg gcaaggccca
    46021 ggaagaactg gggcatgaag gtcaggttga agctgacgac cgtgagccag aagtgcagct
    46081 tgccgagccg ttcgctgtac atgtgaccgg tccacttggg cagccagaaa tacacaccgg
    46141 tcatcacgcc catgatgccg ccggcaaaga gggcgtagtg gaagtgggcg acgacaaagt
    46201 agctgtggtg gtactgggca tcggcggcca catcggccag caccaggccg gtgaggccgg
    46261 cgatgccgaa cagtacgatg aagcccacgg cgaagagcat cggcgtctcg aagctcatgg
    46321 cgccgcgcca catggtggcg agccagcaga agaacagcac cgccaggggc agggagatgg
    46381 acatggtgct gtacatgaaa tacagcagag ctggcacggg caggccggcg gtcaggaaat
    46441 ggtgggccca cacgatgagg cccaacacgc cgatggcgat gaaggaccag acctgggcgg
    46501 tatggccata aaccggcttg cggctgaagg tggacagcac gtggggcatc aggccccaca
    46561 ccgggagaag caggatgtac acctcggggt ggccgaagaa ccagaacagg tgctggaaca
    46621 ggatcgggtc gccgccgccc gccgcgtcga aaaagtgggt gccgaagtgg cggtcggtga
    46681 ggagcatggt gacgccaccg gcgagcaccg gcagggtgat gatgagcagc accgcggtga
    46741 ccagccagcc ccagcagaag aggggcatct tcatcagcgt catccccggc gcgcgcatgt
    46801 tgaggatggt ggcgatgatg ttgatggagc ccatgatgga gctgatgccg aggatgtgga
    46861 tcgagaagat cgcgaaatcc acgccgatgc cgccctgcac cgagaggggc gcgtagagcg
    46921 tccagccggt ggccacggcg ccgtcgccga tgccgaagag ggccagcacg aagggcatcg
    46981 tgagcagcat ggcagccggc ggcagcagcc agaagcccca gttgttgagg cgcggcaggg
    47041 ccatgtcggg ggcgccgatc atcagcggaa tcatccagtt ggcaaagccc gtcgcggcag
    47101 gcatcagggc cgcgaagatc atcaccagtg catgcacgcc gatgagctgg ttgaagaact
    47161 ccggcttcat cagttgcagg ccgggctgga agagttcggc ccggacgccg aggatcatcg
    47221 ccccgcccac gaagaacatg atgagggcga aggtcatgta catcgtcccg atgtccttgt
    47281 ggttggtggt ggtgagccac cggaggagac cggtcgggtg gtgggcgttg tcgccgtggg
    47341 cgtcgagggc gtgggtggcg gtgttcatgg ggtgctcctc agcgaagggc cttgatctgt
    47401 gccggctgga ccatgtcacc catggcgttg ccgaaactgt tgcgctcgta agtgacgatg
    47461 gcggcgatgt ccgtgtcgga cagggtgttc ttaaaggcct gcatggcggt gccggccttg
    47521 ccgttgagca cccggtccag gtggctgtcg gggatcagct tgccctcggg cgagagcagc
    47581 gggctgttga ccaccttgct gccggccagc gccgggaagg ctggcgggac ccccttgccg
    47641 tcgggctggt ggcaggcagc gcaggtcttg tcgtaggctt ccttgccctt ggccatcagt
    47701 tcgtccttgc tccaggtctt gtcgctgccg gcgctggcgg cggcctgggt cgccttcttt
    47761 tcgtccagcc aggccacgta ttcggcctcg ggcacggcgc gcaccaccac gggcatgaag
    47821 ccgtggtcct tgccgcacag ctcggcgcat tggccgcggt agatgccggg cgtctcgatg
    47881 cgcacccagg tctcccgcag gaagccgggg atggcgtcac gcttcacgcc gaactggggt
    47941 acccaccagg tgtggatcac gtcggtggag gtgagcagca cgcgcacctt cttgcccacc
    48001 ggcaggacga cctcgttgtc cacctccagc aggtagtgtt cgcccttggc ttccttgccc
    48061 tcgatctgct cccgcggggt ggacagggtg ctgacgaact tgacgccggt gtccgggtat
    48121 tcgtactgcc acttccattg catgccggtg accttgagga ccatgtcggc cttggtcctg
    48181 gtgtcttcca tgtcgatcac cgcgtggaag gcggggatgc ccatcagcac gaagtcgatg
    48241 aagagcagca tggcgaaggg caccaggacc cagaaccact ggagcgctcc cgaaggcccg
    48301 ctgaatccgg cgggctgcgc gccagtgctc ttacggtgct tgaccatcga ataaatcatg
    48361 atggcgaaga cgaccacgaa cagcgccgtg atgatcacca tgaactggtt gtgcatcgac
    48421 agcgtgtcgc gggcgatggg ggtgacgggc gtgggaaagt tccacgtgta gtcggcgaag
    48481 gcaggggcgg acagcgccag gccgatgccg gccgcaaaga atggccggcg aagggtgggg
    48541 agtcgggtgg ggatcaagtc tcttcctctt ctgggcatgg acgggagggg gtcagggcag
    48601 ccgttgaagc tggccgcgca gttcggcagc caatgtgcga cgctggtcgg gcgtcatcag
    48661 gtgtcccacg cgggcgtggc ggccatggga gcggatgtac agcagggggt cgccgccggg
    48721 gccgtcgggt tcaaggctga cccgcgccca gaaaggatgg aagctggcgc tcaggtcgcc
    48781 gtgcggacag tgggtcagca cgctgagacg gccggattcg aggcggatgg tttcgtaggc
    48841 agtgtcgctg cgctcaaggg cgcgcagggc cagggcgagg acgatcagtt ccagcccggc
    48901 aaagggcagt accatccagg cgcccgccag catgaaccct gcgctgacga gcagggtcac
    48961 aatgacaagt ccgccgatca ggccgcggcg ggcagcagaa ctcagagagc ggttggggcg
    49021 cgcggtccat tcccgcgcgc cgcccgcttc ttgaaaggtc agagccatgg cgtctcctcc
    49081 aggctttgtc tgcgactttt tgacgcagat caaatttgtg ggcgcagttt agcatccgct
    49141 tacagttttg cggtgcaccg gattttttgc gtaacctgct gattaaaaat gactaaccat
    49201 acttttgcga tgttgcggtg cgcaatttca ggtgttctgg cggccatgct gctggcggga
    49261 tgcacgccgt ccggcgaggg gggcgggaag gcacagggct ttgtggcgac ggacattacg
    49321 ggggcggact atgcccgcgg gttccggctc accgatcaca cgggccggcc gcgcacgctg
    49381 gaggactttc gcggcaaggt ggtgacgatc ttcttcggct atacccagtg ccccgacgtg
    49441 tgtccgacca atctgctggc gatggccgag gtgatgcgcc agctcgggga cgacgcggca
    49501 cgggtccagg tgttgttcat caccgtcgat ccggagcggg atacgcccgc cctgctggcc
    49561 cagtacgtac cgtcatttga tgcgcgtttc ctgggcctgt acggcgatcc ggcggcgact
    49621 gcggccgtgg ccaaggaatt ccgcgtgttc taccagaagc agggcgacgt gaagggcggt
    49681 agctacacgg tggaccattc caccgggacc tacgtgttcg atgcctccgg ccgcctgcgt
    49741 ctgtacgtaa aacacggtga gcagccggac cgcatcgcgg ccgatctccg gcgcctgttg
    49801 gcagggcagt gagtcggccg gtcgtcgcgg gagccggcgg tgccgcgtgg gtgtccgcgc
    49861 gcccgggtgg ggcgcgctgc ggccctgaat gaaaaagggc agcccgcagg ctgcccctat
    49921 tccgacggtg gcgtcgctca ggcgaaggcg gcgagttgcg tacgcatctt ctgcatggcc
    49981 ttggcttcga tctgacggat gcgctcggcc gatacgccgt attcggcggc cagttcgtgg
    50041 agcgtggcgg cgtcgccttc actcagccag cggcggctga cgatggcacg actgcgctcg
    50101 tccaggctgg ccagggcgtc gtgcaggccg cggtcctgca ggcgggcaag ctgctgctcg
    50161 gccagcacct cggacggctc ggcgtgcggg tcggccaggt aggcgatggg ggcgaagcgc
    50221 tcctcatcgt cgtccggccc ggcgtccagc gccacgtcgt gaccggacag gcgggtttcc
    50281 atctcccgga cctcctcggg cttgacgccg agctgcttcg ccacggccag tacttcgtcg
    50341 ccctgcaggg tggcgctgtc gtgcttcatg ccgcgcaggt tgaagaacag cttgcgctgg
    50401 gccttggtgg tggcgatctt gaccagacgc cagttgcgca cgatgtattc gtggatctcg
    50461 gccttgatcc agtggatcgc gaaggacacc agacgcacac cgcgcgttgg gtcgaaacgc
    50521 ttgacggcct tcatgaggcc cacgttgcct tcctggatca ggtcggcgtg gggcaggcca
    50581 tagcccaggt agccgcgggc gatggccacc accagacgca ggtgggacat tacgagcttg
    50641 cgggccgcgt ccaggtctcc gtcatcccgg aagctgtggg cgagatcgga ctcctccttc
    50701 tcggtcagca gcgggatgcg attgacgctc tggatgtact gatccaggct gccgaccgcg
    50761 gagggaacag gaaacgacag ggttgtggtc atgggtaagg ctctccttgc gcccaaagtt
    50821 tagcactcca tgccagagag tgctaacagc gccaggagtt cccgtgccgc gggtcggtgc
    50881 cgcgggaagt ccgccacgcg tgttgccggc cctcaccggc cggggccccg gcttcccccg
    50941 cgggcggggg accggggcgc ccgaccggga gggtgatcac aggccgagga cggccttggc
    51001 gcgggtcaga tagtggatgc ggtcgtccag accgttgtag ccgccgttga tgcgcttggt
    51061 gaccgccttc acgtcgtcct gctcggccag cgcgggcagc ccgcgggtga cccagaacca
    51121 gcaggccaca tccacggcgg ccagcggatc gtcggcaatc ttttcagggt gcgccacgta
    51181 atcgacgccg caggccttgg agtacgcagt gtagttggcc cggccggtca gctggatcag
    51241 cccgcgccct ttgtagcgcc ggccgtcgcc cgtctgggtg ttgcccaggt ccttgcggcc
    51301 ttcataggca tcgccgctgg cgatctcttc ggtgtacagg aacgaggcgg attcgtgggc
    51361 gacctgggcc aggaagtggg ccgcctgcag gggcgtggtg atgccgtact tggtcatgcc
    51421 ggacttgagc ggatcgaagt aatggtcgat gcgctcggcc ggcgagaggg gcagcacgat
    51481 ggacagcttc tccttggtcg gcccttccgg caggccggcc agcagggcgc ggacggtggc
    51541 gtcgccgggg gcgatcaggc cgtcggactg gggcaggccc atggcctgca cttcgaagtc
    51601 ggagatgcgc tggatggtgt tcttgccaat gcggccgtcc acccccagcg gtggggggtt
    51661 gggcggtttg aagcgggtgg cgttgaggtt gagcaggatc tggatggcgg ccacgtcggc
    51721 gaggtcgtta cggccgccga gtccgacgga ggtggcgatg cacatggtgt ttctcctgga
    51781 gggcttgcaa aagggggcgc gacctatccg gtatatatca cccacccgcc atgtcaaatc
    51841 cgggtccgcg ccgggcgcac tgccgtgaag ggcctgaagc cctcgccgcg cagggcgagc
    51901 cggcgccgga ggcgcccgcg tcagaccgcc ttgagcgagg ccatgtcgat cacgaagcga
    51961 tatttcacgt cgctgcgcag catgcgggcg taggcggcgt tgatgtcctg catgcggatg
    52021 atctcgacat ccgaggtgac cccgcgggcg ccgcagaagt cgagcatctc ctgggtctcg
    52081 ggcaggccgc cgatgaagga gccggccacc gccttgcggc ccaccaccag ggacgtggtg
    52141 ttgagcatcg catccagctt gccgaggaag cccaccagca ccagggtgcc gtcgtggcgc
    52201 agggtgggca ggtaggggtt gaggtcgtgc gcgtagggta cggtgtcgat gatcaggtcg
    52261 aagcggttgc gcacggcctt catctgcgcg gcatcggtgg acagcacgat gtggtcggcg
    52321 cccaggcggc gggcgtcggc ttccttgccg ggcgagcggg tgaacagggt gagttcggcg
    52381 cccagggcct tgcccagctt gagggccatg tggccgagac cgcccaggcc caccacgccc
    52441 acgcggcggc ccggtccggc ctgccagtgg cgcaggggcg accaggtggt gatgccggcg
    52501 cacagcaagg gcgcggcgcc ggccagatcg aggccatccg ggatgcgcag gacgaagcgt
    52561 tcggacgtga cgatccgctc cgaatagccg ccctgggtgg gcgtgcggtc gaggcggtcg
    52621 gtgctgttgt aggtgaaggt cgggccttcc tggcagaact gttcgagccc cgccgcgcag
    52681 gcctcgcagt ggcggcaggc gtctaccaga cagcccacgc cgacccggtc gccgggccgg
    52741 aagcgggtca ccgccgggcc cacgctggtc acacggccga cgatctcgtg accgggcacc
    52801 accggataga ccgtgttgcg ccagtcgttg cgggcggtgt gcaggtccga atggcacacg
    52861 ccgcagaaga ggatctcgat cgtcacgtcg tcgggtcgcg gactgcggcg ggcaaagcgg
    52921 tagggcacca ggtcgtcgct gggagagtgg gcggcatagc cgcggacgtc gaaggtcatg
    52981 gtcgtcgctc caggagtcgg ggtcggccgc cgggccacgg ccgcagcggc agtgtacgcc
    53041 cggcgtacat ctctgggcgg tgccggttcg ggcgccggcg gcggcgcaac aaaatcgtca
    53101 tgaaatctaa atatcatgcc tgttgatgaa acagttcgac actcggacga ggagcgtggg
    53161 gtgaacgtcc gcaagcatct cgcccccgcc ctcctgaccg tgctggtggg cgccatcggc
    53221 ggcggcatct ggtattccaa cgggcagctg ggcgacgagc acgcccagcg tcaactggcc
    53281 agccagacgc aggcccgcca cgtcaccctg cgcgggctga tcggctcgga gaaggaggcc
    53341 ttcttcaacg atccgcgtgt gcgccaggca ctggaggccc agggcatcgg cgtgaccgtg
    53401 gagaaggcgg gttcgcgcac catcgtggcc cagttcgatg ccgcccgcta cgacttcggg
    53461 ctgccctccg gcgcaccgga tgccgagcgc ctgcgccagg tctccaaggc caagcgcgtc
    53521 ttcaatccct tctacacgcc gatggtgatc gccagctggc ggccgattgc cgacatcctg
    53581 gtggccaacc gcatcgtcga gcgccagggc gagctgtact acctgaccga tctgccccgc
    53641 ctcctcgact tgatccagca tggcacgcgc tggcgcgagc tcaaggacag cgggcgtttc
    53701 tccaccagca agtcggtgct gatcaactcc accgacatcc gcagctccaa cagcgcggcc
    53761 atgtacctgg cgctggccag ctatttcggc aacggccagc aggtggtgca gagccaggcg
    53821 gacgtggagc gggtgctgcc cgtcgtcgca ccgctgtttt tgcgccaggg ctttcaggag
    53881 cagtcctcgt cggcgccctt cgaggactac ctcgccctgg gcatgggcaa gacgccgctg
    53941 ctgttcgcct acgaatccca gctcgtggag ttctggctgc gccatccgga ccgccgcaag
    54001 ggcgacatgg tgatgatcta cccgcgcccc acggtgtatt ccaagcacgt gctggtgccg
    54061 tacacgccgg ccggcgaacg gctcggggtg gtgctcgaga gcgatcccgc cctgcgcgcg
    54121 ctggcccacg aatacggctt ccgcaccggc ggcgacgtgc atggccccga ggaatgggcc
    54181 agacagggca tcaccgtgcc cgacaccctg gacgacgtga tcgacccgcc cagccaggaa
    54241 tggcaggagc gtctgatcca ggccatcgaa acccgcttca agtaaggagg cgccttccat
    54301 gacaacccag aaccccgctg ttgccccggc cgcgagcctc gacctgccgc cgcccgacgc
    54361 gtttgcgtcc ctgaccctgg aagcgcccgc gccggtggcc ccggtgccgg tggcgtcggc
    54421 cggcggcaag gtgcgcctga aggccgaaga cgtgcacgcc ctggaccaga tggccggcca
    54481 gttcatcgac gagatcaccc gctacgacag ccacgatccg cgcttcaagt cggcggtgga
    54541 gcgtatccac gccatgggca accgggatgt ggaagcgtcc gcgtcggtgt ccaaccgcat
    54601 gctcaaccgc ccggtgcaga cgctcaagaa tggcatgttc gacaagagca gtctgatcgg
    54661 tgccaacctc atcgacctgc ggcgcaccat cgaggatctg gacccgtccc gccagcacga
    54721 tctcttcaag ccgcgcaaga ttctcggcct gatccccttc ggcaacacgc tgatcgatta
    54781 tttcgaccgc tacagctcgt cccagtccca catcaacgcc atcatcgaag gcctcaagcg
    54841 cggccgcgac gagctgctcc gcgacaacgc ggccatcgaa caggagaagg ccaacctgtg
    54901 gggcctgatg gagcgcctcg agaagtacat ctacatcggc cggaagctcg acggcgagct
    54961 ggacgccaag gccgcccagc tcgatctcgc cgatccggac aaggcccgcg tggtgcgcga
    55021 ggagatgctc ttctacgccc gccagaaggt caccgacctg ctcacccaga tgagcgtcaa
    55081 catccagggc tacctggccc tggacatgat ccgcaagaac aacctggagc tcatcaaggg
    55141 cgtggaccgg gccaccacca ccaccgtggc ggccctgcgc accgcggtga tggtggccca
    55201 ggccctggcc aaccagaagc tcgtgctcga ccagatcacc gcgctcaaca ccaccaccgg
    55261 caacctcatc gccgccacca gcgtgctcct caaggagcag agccaggcca tccacgagca
    55321 ggccgcgtcc ggcacgatcg agatcgccaa gctgcagcag gccttccaga acatctacca
    55381 gaccatggac gccatcgacg acttcaagct caaggcgctg gcgtccatgc agaccacggt
    55441 ggagaccctc ggcagcgagg tggaaaagtc ccgcagctat ctcgaccggg tgcgtcggcg
    55501 cgaggcgggc aacaccttcg aggcttcgtc ggccggagtg agcctgtgag ggaccgccgc
    55561 cgcgggtggg ctgcggtcgg ggccgtgctg ctggcgctgg ggctgacggc ctgtgggcgc
    55621 gagtcgggcg gcggggccgc cgggaccgcc caggggccgg tgaccgtcct cgccggctcc
    55681 gagctgcgcg acgtggagcc ccagctcctc aaggcggcag ctgatgcagg cgtgccggtg
    55741 aaactcagct acgccggcac cctggacatc gtggagcggg tcaatgccgg cgaagcggtg
    55801 gacatgatcc tgccgcccag cggcgcctac ccgtccctcg ccctggccac gccgcccagg
    55861 gcgcgggaaa agctcttcta ttcccgcgtg gtgctgggcg tgcggagcga caaggccagg
    55921 gccctgggct gggaccggcg ggcgccggcg tggacggaca tcgcgcgggc ggctggcgcc
    55981 gggcgactgc actttgcgat gaccaacccg acgtcgtcga acaccgggat gagcgccctc
    56041 ttcgcggtgg cttccgcggt ggcgggcaag accgaggatc tgagcgaaaa agaggtgaat
    56101 ggcaccgtcc tgaaggcctt cctggctggc cagacgctga ctgccggcag ctccggctgg
    56161 ctcgccgaag cctatgcccg cgacccctcc gctctggacg cgatgatcaa ctacgaagcg
    56221 gtgatcctgc gcaccaacgc cagtctggcg gacaaggacc gcctcgtgat catccacccc
    56281 cgcgacgggg tgatcaccgc ggactacccg ctgatgctcc tcaacgagcg tcggcgcgac
    56341 gcgtacacac gcctcgtggc ggccttcaag tcggtcgcgg tgcagcagaa tctggccgcg
    56401 agcgccttcc tgcggccggc cctgcccgac gtgcccctcg ccgccggcgt atcgccggag
    56461 cccgtggtgg agctgacctt ccccaaccgg ctggaggtca tcgacgccgt cctgcaggcg
    56521 gcgcaggccc aatggcggcg gcccgccacc tccatcttcg tgctcgacgt cagcgattcc
    56581 atggccggcg cccgcctcga cgccatgcgt caggcgctgc tgacgctcgt ggaggcgaag
    56641 gggctggcgg ccagccagcg ctacgccggc tttcagcccc gcgagcgggt gatcatgatc
    56701 cccttctcgg acaaggtctt cccgccgacg caggtggact tcggccagga aaccctcgac
    56761 gacgggcgtc ggcagctgcg caccttcgtg aacgcgctgc atatcgctgg cggaacagcc
    56821 atctatgcgg cgctgatccg cgccgacgag ctggccgacg cggagctcag gcagtcgccc
    56881 gaccggaccg tgagcatcgt gctcctgacc gacgggatca acaattccgg gccgggctac
    56941 tacgaattca tgcggcaccg tggcgcggac gccgtccggg tctttcccat cctgttcggg
    57001 gaggccagcg tcgaccagat ggagtccctg gcctcggcca cccgcgggcg cacctttgac
    57061 ggacgccaca ctgccctggc gagcatcttc aaggacatcc gcgcctacca atgaacgacc
    57121 gctcccgccg cccggcgtcc ctgcgtgcgc tgctcttcct ctacagcaac gccaacatcg
    57181 gcggctgcct gagcgcgctg ctcgggccgg ccctgctctt cggcggcgtc atcggtcccg
    57241 gctggatgtg gatcaccgcc ggcctgtacg gcgtgggcgc cctgggcggc acgctgctgc
    57301 ggcggacgcc cgaggtggag cggcagatcg aggcgacgct caccctcgac gagatcctcc
    57361 agcacctgga ccgcgtcatc gccgaggtga cgcccgaggt cgggcgggaa atcctgacgc
    57421 acctggcgag cctgcgcagc gccatcgccg aggtgctgcc gcgcttgctg gagctgtccg
    57481 gcgaccatgg cgacctctac accgtgcgcg agacggtgct gcgctacctg ccggagaccc
    57541 tcgccaacta cgtggccctg ccgcccgcct tccgcagcgc ccacgtgctg cgggacggca
    57601 agaccgcgcg ccagctcctc accgagcaga tcgccctgct cgacgcacgg atgcaggaca
    57661 tcgtggccaa cgtggcgagt gccgatgccc aggccctgct cgccaacggc gagttcctgc
    57721 gcaccaagtt cgattccgcc gacttcctgc cgcgctgaac ggcgcgtcgg gcgctgcttc
    57781 agggcgccgc ggtgcgggcc cgcagcgtct cgccgaaccg gcccagctcg ccggcgtcca
    57841 gatcggagat cacccggaag cccatccggc cattgctcca ctgcagcgtg ttgaagccac
    57901 ggatctgggc ctggcggagg accgccacgc gctcgtcggg ccacacgaaa acattgatga
    57961 cgtgcttgcg gtgcgtgtag gcaaggcccg ccacgcgcgt gccggcgagg gtgtccacac
    58021 gcccgccgag gagcggaaag cccacgtccg ccagatccgc gaccggcggc gcgtaatcga
    58081 tgcgcccctg gaaccagggc ttgacggtgt gccgatctgc cgacgcaacc tcgatcagcg
    58141 gaccgctgcc caggctgcgc acatgactgg cgaccacggc ggcctcggcg gagcggtcct
    58201 gctcgatctg ctgcacccac ggcacaccca ccagggtcgc gagcacgccg aggatgaagc
    58261 cggcggccat cccgcgccag cgcaggacgt cgggacctgc cgcccgggac agggcgctgc
    58321 ctgccgcacc gcgcaggccc gccagcagga tgcggctgcg cacgcgggcc tgcagaccgg
    58381 gcccagcgcg atggcgggtc gcgtgggtgt tgagcagggc ggacagttcg tcgtcgttcg
    58441 gactcatctt caggactcgc tttccagggc gccgagaatg gcccgcaggc gctcgcgggc
    58501 gcgggacagg cgggacatca ccgtaccgac cgggatcgcg gagatccggg cgatctccga
    58561 atattccagg ccttccagtt cgcgcagcac gatgacctcg cggagcggcg gcggcagggc
    58621 gcgcagggcc gcgtcgatcc gcgcgcgcgc ctgccggcct tccacaaccc gccccggctc
    58681 cggcgcgctg tggccggccg cggcgtgggc cgcgtccagc gctgcctcgt cgaagccgcc
    58741 gagctcctgg cgattgcggg tgcgctccag atgactgaag caggtgttgc gcacgatgcc
    58801 cagaaaccag ggccgtatcg cgtcgccgcg ggcgctctcg aagtagcgga aggcgcgcag
    58861 ggccgcctcc tgcaccgcat cctcggccgc cgcccggtcg gtcagcagcc agcgggccag
    58921 attgtaggcg gcgtccaggt gcggcagcac gaggctttcg aaacggcggg cggcgctcac
    58981 gacggatcag gcacggacga tcaggcgtcc gtgcatctcc tcgtggccgt cgccgcagaa
    59041 gttgtcgcac aggaagtcgt aggtgccggg cggcaggggc ggcagggcga cccgtgtcac
    59101 ccgccccggc ggcagatcgg tgcgcaggcc gaggtccggc aggctgaaac cgtggacgaa
    59161 atccaccgag cggatcgcca gttccaccgc ctggccggcc ttgagcggga tctcggccgg
    59221 cgtgaaacgg aagcggcggg cttccacgtc gatgacgggc ggcgtgtcgg ccacggcatt
    59281 gcggccggtc agcccttcca gcagggtggc ggcgccgagg ctgagggcgc cgcccaggag
    59341 gcggcgtcgg gtccaggtct tcatgagcgt tgcaccggga attcttggag gccgaaagcg
    59401 ctgccgccgg cggcagtatg gacttcccgg aagccgaggc cgcggtaagg ttcggcggca
    59461 tcccagggta atggcgtgcg tttctcctgg gcgccgggcg ccggcagcgg aaagatcagg
    59521 gattttgcgg aatggtgcgg gatgtggccg gtcatgcggt gatgctcctg gtggatgtgg
    59581 ccatagaaca cggtcacgtt gcgccagcgt tgcaggatcg ccatgacctg cgcgccgtcc
    59641 cgcgtggccc aatcccagcg gggcgccagg tcgaacaggg ggcgatgggt gagcacgacg
    59701 atgggggtgt ccgccgcaag gggcgcgagg gtcgcctcca gccagcggag ctggtcttca
    59761 ccgacgcggg cgccggggtc cgagacattg tcgagggcga tgaaatgcac gccctcgtgg
    59821 acgaaggtgt aattcgggtc gccgaagtgt tcccggtagg cggcaccccg gtcgagggag
    59881 gcgtcgtgtt caccggggag atagtagtgg gtgcggacct gcaggtccgc cacgatggcc
    59941 ttgaactcgc gcatccggcg ccggcgctcg gccgggtcgt cggtggtgtg cgtcaggtcg
    60001 ccggtaaaga cgatgaaggc cgggggcact gccagggcat tgaccgcctg caccgtcttg
    60061 cgcagcgtga tggccgcatc ggggttggcc tctccctgat agccccagtg tacgtccgac
    60121 atctggacga agtagaagtc ccgcgtggcg ccgggcgctg tggccagccc ggcgcaaccg
    60181 gccagtccgg aggcgaatac cacgccgccg ccgagggcgc tcaggcccag aaaatgccgt
    60241 cgatccatgt ccttgctcct gttgggtgac ggacgcccgc tgcgccctcc ctgtgccata
    60301 ccggatgggg cgcgggttta ttcccgcggg gcggtctctt tttgcggcgc ctcgccgagg
    60361 gtgcgcagat aggcgatcag atcggcccgc tcggccgcat cggcgacgct cacccccatg
    60421 cgctgccccg gacggacggc ctccgggtcg gtcagccagc ggtcgaggcg ctcgggcgtc
    60481 cagacaaatc cggcgtcctt cagcgcaggg gaataggcga agccgggcac ggtgccggcc
    60541 aggcggccga cgacgccccg gtgggcgggg ccggcgccgt tgaagtcggg ggcatggcag
    60601 gcgctgcagt gctcccgata cagggcacgt ccccgctcgg gcgcgtcgtc ggcgtgggcg
    60661 ttcatggcgg cacagagggc aagcccgaac agggcgaggg agaggaggcg gtgcatgacg
    60721 gaactccagg cggggtgatg gggaggcgcc gacggtgagg ccggtgtcgc cctgtttacc
    60781 gcccgtgagg cggattcatt cccggcgtgg cccgaaggtg gccgacgtgg cttgcctcga
    60841 gactgcctgc accgcggcgg cccgctgacg gccggcctgg tggcgacccg gagtgcccgc
    60901 gggctgcgcc ccgaatcggc aaggcgcggc ccacccactg ctgcggtgtc atggaccgcc
    60961 ggaatgctgc ctcgacgcgc tgagcttttt tggcgatcgc tttttcacgt tcgccatcgc
    61021 acgggtgagc ctcgtggatg tggagcgcca ggcggcggtc tggcggctcg cgagacgtgc
    61081 cgggaggtcg gcttctgcgg acatgccagg cctgccgtcc cgcgtacggg gggtggacgc
    61141 ccggcgcaat gcacgtttcc gcgacaatcc gcgggcggca ggggcccccc ttcagctgcg
    61201 ccgatgcggg cgtaccgctg cgtctgcggg gcggcgagtt cgtcggcacg ctgtgcctga
    61261 tcggcccgca gcccggcact caggcggaac gggccgtctg gcggtgggac tggcgcgtgg
    61321 cgccggcccc tgacgcgccg cggcggtgtc agcgcacgaa ttcgatgcgc ttggcggggt
    61381 cgacgctgtt caggtagtag gcgccgtcgt cgcccttgac gatggcgctg caccattttt
    61441 ccgagacgcg ccgccattcg atctgcacgc agtacttgcc gtcgtcggat atgttccagg
    61501 tgccgggggc ggtggcattg gaggtgcccg cggcgctccc gtacttgcgg ttgtccgagg
    61561 aggcgacgaa gctgccatcc gccgcattct tccagctccg gacgctgccg gcgctggacg
    61621 tatggtgggt ttcgctgccg ggaatcaggg cctgcagttc gtcccggctg agccgaaccc
    61681 cctcgccctc ggcccaggcc agcggggtgg ccaggacgca gccggcgacg agaaggtgaa
    61741 gtgcgcaggc aatgggaatg gggcgtgcat ccatggtgac gatcctcctg acagggtgtg
    61801 gttcgggcgg tcctcggccg ggtctgcgct gaagcgtcgc gctggcggac gacgtccagg
    61861 gggacgggag ccctgcgttt catgatacgc ccgccgccgc ccggaggcgc ccgggtgcgc
    61921 cggcttgtca cgcggtcgtc aaggaactga ccctaaactg taccgagcct gtaacccagg
    61981 cgggctgcgg cgggactaca ttcaaagtag tcgatgattt catcggtcgc gtgcggagca
    62041 catcatgacg aaccgcagcc tgcagtacat cgtgaaggat caggagcccc tgacgcgggg
    62101 gccggaggcg agcctgcaag aggcctgcca ggacatgtgg acgcgccgcg tcggggccgt
    62161 gatggttgtg gacgacggcc ggctggtggg tatcttcacc ggccgggacg ccgtccatgc
    62221 cctgtcccgg ggcttcgatc cgcggcatac ccccctggcg acggcaatga cgccggcacc
    62281 ggacacgatt ccgccagagg ccaccgccat cgacgccctg cgccgcatgc gtgactgcgg
    62341 ttatcgccac ctgcccatcg tggaccgggg gcggatcgtc ggcatcgtct cgcggggcga
    62401 cttccagggc ctggaactgg accgcctgga aggcgaggac gcgctctggg agcgtatctg
    62461 ctgaatggcc cggccggctt ccggcgcgcc gggtgggcgt gagggtgggg gagggaggct
    62521 tcgtgcgggc acgttggggg acatggccga cgtggcgcct gccgctgcac gttccggtgg
    62581 ccgttctgtt ttcgctgctg atcctcgggg tgggcacggc gatctccctg tatcacttcg
    62641 gtgagacgcg ccacctgctg gatgcggcgc aggcggccct gttccgcgcc gtcgtggcgg
    62701 aggtggggcg cacggtggac gacgcggatc gggcggtgcg gcgcagcttc gtgctgctgg
    62761 cggcttcgtc cctggccgac gccacccgct ttggcgagcg ggtgcgcgat ctgccgacgc
    62821 tgacgggcct gctggaggcc gatcctgtcg tcgactcggt gatgatcggc tacggcgggg
    62881 gcgacttcct gctggtgcgg cacgggcgcg gggaggtggc ctgggaggtg gtggacgtga
    62941 gtcgtcccgc cggtgtggac gccgtgccgg tggtcatggt gtcccgctat gacgcgcatc
    63001 tgcgcctcct cgatgcgcgg gccgaggaca atgtgtccgc ccgctacgag ccgcgtctcg
    63061 ccgagtggta ccgactggcc gcagcgcagg acgccctggt ggtgacggcg cccgggatgc
    63121 tgcccttcgc gtcccgtccg ggtgtcacct acgcccggcg taacggggcc gaggtgttcg
    63181 cggtgaacgt ggggctggac aatctggccg ggctcctcgc ccgcgcgcct gtgccggcgg
    63241 gcagccgtct ccagatcgcc gacggcgccg ggcgcgtcgt ggcgagcggc ggccgtccgg
    63301 acggggcggg cgcggatgtg atggccgcca ccttgcggat gtccgccggc tcgaccgccc
    63361 gccggtcggt gcacgatgag acgggcgccg gctggcaggt gtccgccgca ccgctgcggg
    63421 gctttggcga gcagcactgg acactggcgg tggctacgcc cgacgcgagt cttttcgccg
    63481 aagccgaacg ccagcgaggt cacgctatcc gcgttacggt gctggcgatg ctgttcagcg
    63541 tgccactcgc ctgggggctg tcgcgcctgc tcacgacgcc cctgcacgat ctggcggacg
    63601 tggcgcgcgg cctgtcggcg ctccagttcc aggcgcggcc ggatacccgc tcggtgatcc
    63661 tggaggtgga ccagctggcc cgcgccatcg gtgtgatgcg cggtgccatc gcccgctttc
    63721 tggagatggg gcgcgatctg ggcgcggcgc gggatgtggg cgcggtcctc gatgacgtgg
    63781 tgaacgcagc gcgggatgtg gcgggggcac gcctgtgccg ggtcgaggtg gccgacgacg
    63841 gggaaggttg cgcggaggtg tcgcgccggg cgaccggtca gcccttgccg gcgggcccgg
    63901 cgctgcaggt gctgagcgcg ctggtgtatg aggccgaggg gcctgtgagt cttgccgacc
    63961 gcaggacctc cggcccctgg cagtacctgg gcattcccct gcgcacaccc gatggcgagg
    64021 cgctgggggc gttgttgctg gcggaccggc aggggccgcg cagcggtctg gcgcgggccg
    64081 aggtggcggg cttcctgctg gccctcgccg gcagtgccgc ggtggccctg gaaaaccagc
    64141 gcctgttgcg ggggcgcaag gcgctgctgc gcggcgtgat cagcatggtg gccgaggcca
    64201 tcgatgcgaa gtccccgtac acgggcggcc attgccgccg ggtgacggcc ctggcccagg
    64261 gcctggcgcg ggccgccgag gcgtcttcgc agccgtccct tgcccagttc cgcctcgacg
    64321 ccaacgggca cgaagccctc gagatcgcct gctggctgca tgactgcggc aagctcacca
    64381 cgcccgaata cgtcatcgac aagggcagca agctggagac cctgtacaac cgcatccacg
    64441 agatccgcac ccgcttcgag gtgctcaagc gcgatgtgga ggtggcgtac tggcgcggca
    64501 tcgccagcgg tggtgacgcg tacgccctgc gtcggagacg ggacgccgcg tggcagtccc
    64561 tggacgatga tttcgctttt gtcgcacggt gcaaccgggg cgccgaaggg gtgtccgagg
    64621 ccgacctgac ccgcatcgac cgcatcgctg cccggcgctg ggtgcggacg ctcgacgacc
    64681 ggctgggcct gtctcccgag gaactggcgc gccaccacgc gccgccgccg cccctgccgg
    64741 tcgcagaagc gctgctccac gacgggcccg aacaggtgat cgcccgtacc gacagcgccc
    64801 tgtttgcccg cggcaatccg tggggcttca cgatgacggt gccgtcgcgc ctgtacgacc
    64861 ggggcgaggt gcacaacctg aaggtcggcc gcggcacact gactgaggag gagcgtttca
    64921 agatccgcga acacatcgtt cagacgatcc tgatgctgtc gcgtctgccc ttcccgcggg
    64981 agctggccgc ggtgccggag atggccggcg gtcaccatga aacgccggac ggacggggct
    65041 acccgcgggg tctgcggggt gaccagatga gcgtcgccgc gcgcatcctg gccatcgccg
    65101 acatctacga ggccctcacc gccccggatc gcccctatcg cagcgccaat acgccggcgg
    65161 aggccctgga gatcatgacg cgcctggccg acgagcaggc catcgacgag gcgctgttcc
    65221 gcgtgttcgt ggaggcgcag gtgtggcagg tggacgaccg cccggtcgca cctgcctcag
    65281 gcctgtcagc gatcggccag caggccggtc agcacgacaa gcaggatgaa tgcgatcccc
    65341 aatgcccagc agaccctagt tgagggcttc caggtgcgcc gccggcccgt cccgcggcgg
    65401 cccacgccag gacggcgggt ccacccgtgc gcggctctcg acaatccgtt ctccatctga
    65461 ctcatcacag tgccccgatc ctgagtggtt gtaccgcgag cgtagcgtcc tgagctgaac
    65521 gcagccttca caaaggcttg catggggctg aatgagggga ggcaggggcg ttgacgcgtc
    65581 ccctgcgtgg cagtgccaag tcccggttgg ctgttgcccc cggcctatcg gggcgtgggg
    65641 acggaggcgg attcgcctgg tatgcaggcg tcgggacgtg gccgggccct ccgtttcgac
    65701 cggcagaagc cgcgggtctg cgcgcttgac ggaggcacca aaagcaaaaa ggccaatccc
    65761 ggaagattgg cctaagtgct tgaattctgg tgggttgtga gtggctcgaa cactcgacct
    65821 acggattaag agtccgaagg ttttgaaaag ttgaagttgt attttttcag tgtgatagtt
    65881 gcaaggcatt gaaaatatga gaaatattta aaataagtgt tggctattgt tgatctaggt
    65941 tgatatgctg gggagcaatg ggggagcaac aaagagattg cgcccgggtt tttggagtcc
    66001 tcgccatggc agctcagacg caacgcatcc gactcacggt ggatcgcatc cgccggcttg
    66061 aacctccaac aggcaaacag gcgatctacg tctttgacga cgaccagcgg cacctggcag
    66121 tgagggttac gccggcaggt gccaagtcct tcgtgttcgc cggcaaactc aacaaggtgc
    66181 cgctccgcat caccatcggt gacgtgatgg tttggcagct tgacgac
//
LOCUS       DAOQPA010000007        63564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_158_length_63564_cov_18.310633, whole genome shotgun sequence.
ACCESSION   DAOQPA010000007 DAOQPA010000000
VERSION     DAOQPA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 63564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63564
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_63564_cov_18.310633"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PKX01_01985"
     CDS             complement(<1..1411)
                     /locus_tag="PKX01_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HMZ74918.1"
                     /translation="MDRIDTAETLYLRVAGHYRRAIRSGALAAGDRLPSLRALTRLHG
                     VSLSTVVQACHCLEDEGLLEARPRSGYFVRAARGSTLQPVREPDVGRPPSLPDPAVFA
                     GIHARVSDFVAKCERFPTHTNLAQAFGPPEVYPLEALRNAAIRALRRHPEVLVNPVPP
                     LGDAGFRSVLARRAVAAGMSPAPDEILVTHGCTEALNLALRAVAAPGDVIAVESPAYF
                     GLLQVIESLGLRALEIPTSPSRGLSVEALELAIRTHPDLRAVVVVPNFQNPLGCVMPD
                     GEKARLVELCSGAGVALIEDDIYGALDDDDQSLKACKAWDRDGSVIHCASFHKTLAPG
                     MRVGWMLGGRWLDRIRMLKHAQSRPNDALAQLAIGEFMAGSAYDRHLVRLRRHLLAQR
                     THMAEAIATHFPAGTRLNPPGGGMLLWVELPAGCDSMAVFDAALRSGIRVAPGAMFSN
                     SGRFDHFLRICCGAAYSPSI"
     gene            1583..2011
                     /locus_tag="PKX01_01990"
     CDS             1583..2011
                     /locus_tag="PKX01_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74919.1"
                     /translation="MDAPAPIRLRGGQRHHAHLPAGTLIIACAGRLSVTDAPRWLGDQ
                     VFQVHHRLAEGDVLRIEAGGWIAVSADGDASFVCAPPPGLTLRLHDGAAQLEAWFVQT
                     WRAQVESPLALLRARLRWLQTLQRRRLRVEVSALRARLER"
     gene            complement(2033..2950)
                     /gene="gluQRS"
                     /locus_tag="PKX01_01995"
     CDS             complement(2033..2950)
                     /gene="gluQRS"
                     /locus_tag="PKX01_01995"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HMZ74920.1"
                     /translation="MSAPGGAARKGRFAPSPTGPLHFGSVVAAVGSYLDARSSGAAWL
                     LRIEDVDVPRCVPGAAEGILRTLERLGLEWDGPVLWQSSRTEVYRAALERLMAAGRVF
                     GCICTRRALADACLARDGTRRYSGTCRDGLPPGRTARAFRLRVEPGCVRFVDRIQGPI
                     EEDVAADVGDFVVLRADGYFAYQLAVVVDDAEAGITDVVRGADLLDSTPRQLLLQRAL
                     GLPQPAYAHVPVVTNGAGEKLSKQTLAQAVDGLAPGGVVADALRFLGHPPPAALGGAA
                     PAELLAWAQGIWSLARVPCVRGAPMPGYG"
     gene            3296..5074
                     /locus_tag="PKX01_02000"
     CDS             3296..5074
                     /locus_tag="PKX01_02000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HMZ74921.1"
                     /translation="MVAAGLLACLIALVALWPTVDAPRPLIVWWALVTAGQLGLASIA
                     HRVRTGSGPSALDEDLGHYLLSFGAVVAGAAWGALPLLGPYTSGTPAQLTLFLLLGGI
                     TLGGAAILSASRSAFAVFVLATLLPLFILTVDLPPPNMPHASTGLILFGAFAFAVHDL
                     IHAAHRPRSSPPAAAAVGGFEEKILDGAGEAIVLCTGSRIIHHNRRFIELVGERSTHA
                     RAEGRLAHWLADSSERRRARTALSALCQGRVYRDVVRLRRRDGSIFTAEVSGRALDPH
                     ARPLRVVWVATDITERVAGEVRRTMLGTQLQELVSQSGDGYWQTDALHRLVHVHACHA
                     PLDGILDARIGRPWWTQDPRPGVQSDARQTEIRQIFEDRGAFRDLMVEVRDGYHPPRW
                     LTLSGTPRQDEHGCFLGYHGIAVDVTERVRGTERIRHLAYHDALTGLPNRRLLVDRLG
                     QAIERAQRYRERLGVILIDLDDFKRINDLSGHSVGDRVLLDTADRLRACIRPCDTVAR
                     LGSDEFVVLLSELDQPSDADQMAGTILHALGQPVAGRLGSAPLGTSIGIALYPEDAQT
                     ADSLLEVADRRMYRAKRRGGQTIEHV"
     gene            complement(5159..6241)
                     /gene="aroG"
                     /locus_tag="PKX01_02005"
     CDS             complement(5159..6241)
                     /gene="aroG"
                     /locus_tag="PKX01_02005"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HMZ74922.1"
                     /translation="MHASAKVHIDDVRIKEIKELAPPAHVLREFPATPMAADTTFAAR
                     SAIHRILFGADDRLLVVIGPCSIHDYEAAMDYARRLKAEADRLKNDLLVVMRVYFEKP
                     RTTVGWKGLINDPHLDGSFAINEGLRLARRLLWEVNELGLPAGTEFLDMITPQYIADF
                     IAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIAVDAIKAAQSPHHFLSVTK
                     AGHSAIVSTSGNEDCHLILRGGKGPNYDAASVDAACTELAASGVQGRLMVDFSHANSR
                     KQHKLQVEVARDVAGQLAEGEDRIIGVMVESHLKEGRQDLKPGVPLEYGQSITDACIG
                     WEDSVTVLEMLAEGVRQRRIRRATSE"
     gene            complement(6349..8223)
                     /locus_tag="PKX01_02010"
     CDS             complement(6349..8223)
                     /locus_tag="PKX01_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HMZ74923.1"
                     /translation="MSDPSSHSRAGLLALSLAALGVVYGDIGTSPLYAFKEAFSGPHG
                     IDPSEGNVLAALSAFFWAVALIVSVKYVGIVLRFDNEGEGGVLALTAFAHRVSSNPRW
                     SVLAVAGGIFAAALFYGDAIITPAISVLSAVEGISVATPHLEHAVVPITVGILVALFL
                     IQKHGTGHVGRFFGPVTVVWFLTLAVLGVASIMETPRVLQAIDPRLAVEFAFEKPKVA
                     FVLLSAVFLALTGGEALYADMGHFGARPVRLAWYGLVWPSLMLNYFGQGALVLREPVA
                     IDNPFYMLAPDWFLLPLVGLATLATVIASQATITGAYSMTLQATRLGYLPRLRILHTS
                     DTERGQIYVPSVNWLMLVAVLVLVFQFRSSGALAAAYGIAVSGTMIITTLLTAFIVWM
                     SPTRSRVGLLAVLLLFGLLELMFFGSNLTKIAEGGWLPMVLGGTIFLMLTTWKQGSLL
                     LARKRCQIDVPMAGFIKGPLPDVPRVAGTAVYLLSDSDLVPSALFHNLKHYKVLHERI
                     VFLHVANQDVPRIDDEERVEVTQLAPSIFSVEAKYGFREEPDVPAALALAARYDLELE
                     PMTTTFFVARSNVVDGPGELPRWRCALFAWMVRQSEGAGSSFHLPANQVVELGTQVML
                     "
     gene            complement(8271..8954)
                     /gene="kdpE"
                     /locus_tag="PKX01_02015"
     CDS             complement(8271..8954)
                     /gene="kdpE"
                     /locus_tag="PKX01_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HMZ74924.1"
                     /translation="MSAAQILIVEDEHQIRRFVRTALEADGCVVREADTCGQGLVEAG
                     GHAPDLVILDLGLPDGDGTRFVQDFRTWSAAPVLVLSARSTEHDKIGVLDAGADDYLT
                     KPFSVGELKARVRALLRRGRAHEQVSAVQVFGDVEVDLSRRRVIRQGEPVRLTPLEYR
                     LLTVLLANADKVMTHRQLLREVWGPSYSDSSHYLRIYVGHLRHKLEADPAQPRHFLTE
                     TGVGYRFQV"
     gene            complement(8951..10501)
                     /locus_tag="PKX01_02020"
     CDS             complement(8951..10501)
                     /locus_tag="PKX01_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HMZ74925.1"
                     /translation="MSATMPFYQRDEAPAVRRYGLAVLVCGLAAGLALPLRDVLDLAN
                     TEMLFLLAVVLVAARLGRGPAVLAAVGSVALFDFFFVPPLFSFAVNHSQYLASFAVML
                     AVSLIVGQLTAGLRLQAGEAGQRERTTRALYGLAKSLAGAREPVEVEAALRAFLHAHV
                     DARMWLVLPDAADVLAVVKAEDGPFGELDGHTARMAYDGGCAIESGATEVPGLRRHYL
                     PLAGASRARGVLVVSLPTGGPVGDLPLVEAVASLAATAVERLHFVAAAQAAQLEAVTE
                     RLRSSILSALSHDVRTPLTALYGLADTLLLTRPPLSGEALETAQAIRDQALRVNGMVV
                     NLLDMARLQAGKVVLRSEWQSVEEVVGASLKSLGGALAGHPVRTAGLGDLPLVQCDAV
                     LMERVFCNLFENAVKYSPQGSPLTVTARAGPDTLEIRVGNGGPGFPPDRLEQVFELFE
                     RGTPEAAVPGVGLGLAICRSIVEAHGGTIRAFNPPGGGGCIALRLPLGTPPPVEHEPV
                     AEGSGEML"
     gene            10625..11695
                     /locus_tag="PKX01_02025"
     CDS             10625..11695
                     /locus_tag="PKX01_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HMZ74926.1"
                     /translation="MKRPIPLQGRGSRLNPDTRFADWQRAAFDDGWDAAGDAPVPPPT
                     VLMVDTARSVICRNTSPDVPFSQSINPYKGCEHGCSYCFARPGHAYLGLSPGLDFETH
                     IAWKPDAAAVLRRELAAPGYRCAPIALGINTDGWQPLERRLGLSRQLLQVLAETRHPV
                     SIVTKSALIERDADLLADLARDDLVQVMFSITTLDPDLARRMEPRAASPARRLAAMDC
                     LRRAGVPVGVLCAPLIPALNDHELEGVLAAAKGAGAETAGYVLLRLPRELQALFTDWL
                     QAHFPDRAAHVLSLLRQLRGGKLNDPRFGHRMRGQGVLADLYSQRLARARQRLGLDRP
                     RRTLNTGAFRPPAPDGAQLRLF"
     gene            complement(11722..12249)
                     /locus_tag="PKX01_02030"
     CDS             complement(11722..12249)
                     /locus_tag="PKX01_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74927.1"
                     /translation="MNRWRVVAMAFVLVTGCATQPSPPPPLLRLDPADAARIDAARGA
                     LRPLALDEIVALSRAGTPSADLVARIRATGTRHAIPAPEAARLQAQGVAPEVVDVLNE
                     AQARWAQDQAAAERVRRDTAAAAAEDRARAEAERDRRRAAPYYYDPLWPYGYPYRHGY
                     PGGHFGWGGGVHLWR"
     gene            complement(12263..13051)
                     /locus_tag="PKX01_02035"
     CDS             complement(12263..13051)
                     /locus_tag="PKX01_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HMZ74928.1"
                     /translation="MERREFLMAAAAMAAAAPLGAAPAPAGIALPGAINRAGRQRMLS
                     QRSAKAWLMSVLGVLPDRARSILDQTVTRFDLQLADLKTQLPSDEVRTLLAQLESEWS
                     RYRPLLGDIRSDPKAVWDGNEAVLGVAHKLTVAYEKAFGSPSGHLVALAGRQRMLSQR
                     MAKAYFFRQLNVNAGPAAEMLDQATKEFAKAQAELRGAATNSPQINTELGLVDQQWFF
                     FQNALVLKEGVDPKRAAADVATTSERILEQMDLVVGLYEKLAAR"
     gene            complement(13053..14510)
                     /gene="tldD"
                     /locus_tag="PKX01_02040"
     CDS             complement(13053..14510)
                     /gene="tldD"
                     /locus_tag="PKX01_02040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HMZ74929.1"
                     /translation="MTKNPPPSPLKIASKLLLKPYDLDFEDLDKVFGKLFRHKLDYAD
                     LYFQYHRSEAWSLEEGIVKSGSFNIEQGVGVRAVTGEKTAFAYSDDISLKALKAAAEA
                     TRAIAASGRRKTHKVEAVKKPPLALYRGDNPHGSLDDVAKVRLLERVEAMARAEDPRV
                     REVMASLVGSWEVVLVARSDGHLAADVRPLVRVSVSVIMEENGRREQGSGGGGGRYDY
                     AYFSDEVLAGYARAAVHQARVNLGAGPAPAGSMTVVLGNGWPGILLHEAIGHGLEGDF
                     NRKESSAFSGRIGQQVAARGVTVVDDGTLPDRRGSLTIDDEGNPTQRTVLIEDGILTG
                     YMQDMLNARLMGVAPTGNGRRESFAHLPLPRMTNTIMLAGQHEPAEIIASVKNGLYAV
                     NFGGGQVDITSGKFVFSTSECYLIEDGKVTRPVKGATLIGNGPDCLTRVSMIGNDLAL
                     DPGVGTCGKDGQSVPVGVGQPTLRIDGLTVGGTGG"
     gene            complement(14525..15331)
                     /locus_tag="PKX01_02045"
     CDS             complement(14525..15331)
                     /locus_tag="PKX01_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HMZ74930.1"
                     /translation="MSSVHRIAAVQMVSGPDVAANLAAAERLIGEAAAAGAGLVVLPE
                     YFPLITADETAKLRLCEPDGEGPLQAFLQDAARRHGVWLIGGTIPLAGSDPGRVKNAI
                     LVFDDTGQRVARYDKIHLFGFSKGAERYDEAATIEPGREVVTFDSPCGKVGLSVCYDL
                     RFPELFRAMGEVDLIVLPAAFTYTTGRAHWETLLRARAIENQCYVLASAQGGVHPSGR
                     RTYGDSLIIDPWGEVLARRAEGPGVVVADLDPARIAEVRTSLPALQHRIL"
     gene            complement(15328..19230)
                     /locus_tag="PKX01_02050"
     CDS             complement(15328..19230)
                     /locus_tag="PKX01_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HMZ74931.1"
                     /translation="MTRLRRLLTPFSGERLLSLLHLPRVWACRLLWALIAVYFVAGAG
                     LLALRWAVLPNVPAYREDVAALLSRSLGRPVAIGALSAQWHGFRPALRLDNLTLLDER
                     GEPALTLAQVNTELGWTSLPLLKLRLHRLEVVAPHLELRRDADGRLFVAGLQVNAEGE
                     SGDFLPWLLDQREVVVRDARVDWEDARRGAPALELRQVNLRLENRGSQHRFGLQAVPP
                     SALAATLDVRGDLRGRDPQQPASWRGEVFIGLDYADLGAWKTWVDYPLDLAGAGGVRA
                     WLEFGEARVKGITADFALRDARARLAPELEAIDAMHAEGRLRYADDAGRVEASGKRLA
                     LETRDGLKVAPTDFFLRWQPGRAGTPARGEFVANQLDLGVLAELAERLPVEPGFRRRL
                     ADLAPSGSVAPLNLKWTGEAGALATYSVDAKFAGLGIRPGGAWPGFAGLSGRIEGNDR
                     GGRFSLSGRQAALDLPQVFPESRLALEEMAADGTWSHPDGMLEVNLGSVSFANRDTRG
                     TATGRYRARPDTPGEIDLQARLAEAESTAVWRYMPGVVNQRARDWLQHSLTGGRARDA
                     RLVLRGNLAKFPFRDPKDGVFRVTAKVSDGSLDYAPGWPKIEDVTGELVFEAAGMSIK
                     AQHGRIFGVELKDVSVVLPDFETHEVLSIDGKASGPTQDFLRFIAESPVGARINHFTD
                     GFRTEGVGTLDLHLALPLHRMETSRVKGEFQFARNKVQPDPVLPVLTDAAGRIGFTEN
                     QLVVRSGTALLYGEPASVSGGTRPDGTVLLAIQGNASIAALRRELDEPLLDHLSGATA
                     WKGTIAVPRQGGLEVHVDSGLQGIASSLPEPFNKSPAAAMPLRADYLAGGSTAGDSFR
                     LAIGPSFQMQTARRREGGRWSPLRGGMALDEPLRMADKGVLLAATADRLDVDAWRRAL
                     SGGGAGNGGSGAGGGLPISGVALKAGELRMFGQRLNDVNLRAVMEEGGWQARLASREA
                     TGELTWRDQGRGRLQARFRQLAISGGTSGSGSEAGRSADRLSELPGLDVTADSFVLRG
                     RALGKLDLKAANRGDTWRLEHLSLASPDGTLSGDGSWRPGAKEETRLNFKLDVGSIEK
                     MLARLGYPEAVRQGKAQLDGEIGWRGPPTALHLASLNGHMNVRSEGGQFSQLEPGVGR
                     LLGVLSLQALPRRITLDFRDVFSQGFAFDRISGSIQMNDGVLRTEDLELVGPAARVFM
                     SGDADAVRETQSLRVKVQPTLSESVAVGSALATTGVIHPAVGLAAYLVQKALRDPVEK
                     LFSFEYAVTGSWSDPKVEKIAAVRLPAAPANPAERIGQ"
     gene            19312..21990
                     /gene="glnE"
                     /locus_tag="PKX01_02055"
     CDS             19312..21990
                     /gene="glnE"
                     /locus_tag="PKX01_02055"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HMZ74932.1"
                     /translation="MSEQPDTLHPLVAAATAHSRFLRRQLDSRPWLAARLAASVDAPL
                     DATALRDFLRAEHVDDSTLKPALRRLRAWIIGHVMVRDLAGLASLTEVTETMTLLADI
                     AVEATVDLHRAALVARHGQPVSADGREQEFIVLGMGKLGGRELNVSSDIDLIFVYPED
                     GSTAGPKIIDNYEFFTKLGRAVIQALNDLTGDGQVFRVDMRLRPNGDSGPLVASFDML
                     ENYFITQGREWERYAWIKARVMTGIRGAELAAIVRPFIFRKYLDFGAINAMRELHAQI
                     RQEVARKDMANNVKLGPGGIREIEFIAQVFQLIRGGRDKALQIKPTLAVLGLLVERGI
                     LSRETGRELAAAYDFLRRVEHRLQYLDDAQTHTLPGNPDDQARLARSMGFTHYEALLE
                     ELDDHRAHVGRHFNAVFADPTGKGHDLDTLWSSAGDEALTTAEFTRLGYPDPAAAARR
                     LATLRAGIRYQQMPPSIRSRFDPLIPRVVEAAAPTPDANATFERLLDLLDAISKRGAY
                     LALLQQYPQTLEKVAQIVSASAWAAQFLNRHPILLDEAIDPRILEEELDLGEFHSELA
                     RQLDEIEPDMERQMDLMREQHHAQVFRLLTKDIAGHLTVERHADFLSALADIMVDLAV
                     TMCWRKIRNRHRDDPRFAVLAYGKLGGKELGYASDLDIVFVYDDDAPESAELYTRLSQ
                     RTNTWLSAQTPAGSLFETDLRLRPNGDSGLMVVSLDAFRQYQLENAWVWEHQALTRAR
                     FCAGDRAIGRKFEAIRVEVLRQPRDLAKLRDEVIAMRQRMVDAHPVRAGVFDLKHSRG
                     GLIDVEFIVQYLVLGHAHTHPGLTGNLGNIALLPMAAELGLIPPDDAAAVADTYREYR
                     RLQHALRLNNQAPRVDPALVAEGAAAVRDLWAQVLG"
     gene            22209..22847
                     /locus_tag="PKX01_02060"
     CDS             22209..22847
                     /locus_tag="PKX01_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HMZ74933.1"
                     /translation="MFPYTHYLFDLDGTLIDSAPAILASFREAFARAGVAPVRAIDAS
                     VIGPPLVETLQLLAGRTDAALIDTLAGHFKASYDTTGYQATAAYAGVGEMLARLKGAG
                     RYLAIATNKRLHPTGLILAHLGWDGYFDAVYALDRFTPRLPDKAAMIARLVADEGVPR
                     DGAVYIGDRSEDGESADANGLPFIATTWGYGSLTPADLKPHWRAVNTPAELG"
     gene            22854..23336
                     /locus_tag="PKX01_02065"
     CDS             22854..23336
                     /locus_tag="PKX01_02065"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ74934.1"
                     /translation="MTDTADGAAATAGILAPPPLLYLGAFFAGSLAEALWPTASWAMT
                     GWLRGAGLVLFIASAAFARWAFVTLRRMGTSASPRQASAALGTDGPFGWSRNPIYVAM
                     TGLYLGLTGLLTSPWPLLPLAPLLVAMHWGVIRREERYLHARFGAAYVDYRRRVRRYL
                     "
     gene            complement(23352..23810)
                     /locus_tag="PKX01_02070"
     CDS             complement(23352..23810)
                     /locus_tag="PKX01_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74935.1"
                     /translation="MSSSELPVRFSAAEMEQIEAALTTLERLFMRYYILDSKERYGMG
                     ELGSTGEALCRRMLKIFAHEPLIMPDDFDLLAFRRDLAALDQLRPILARLQTLTDKAT
                     DAEALLNDELSFSVFDGYALVKMIGRPEDFDALLAIMAGTATEDFSGPPL"
     gene            23997..24656
                     /locus_tag="PKX01_02075"
     CDS             23997..24656
                     /locus_tag="PKX01_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ74936.1"
                     /translation="MTAPADPLAAAHAAYTPLSLRVYDAVVMGVSNPLLWKCPTARLR
                     AHFDRHVSGNHLDIGVGTGYFLDHARFPVHAPRIVLMDLSTAALAHAARRLARYRPET
                     VIGNVLAPLPVPGRRFDSASLNYVLHCLPGPFAAKLSAFDHIRPLLNPGAVLFGSTLL
                     QGDAPRNAAARSLMAWYNRKGIFSNAGDDFATLQAALRQRFRQVALERVGCAALFAVR
                     N"
     gene            24851..25924
                     /locus_tag="PKX01_02080"
     CDS             24851..25924
                     /locus_tag="PKX01_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ74937.1"
                     /translation="MRIQSLHPFLSRPVSRHRAPPAPRPTLRALAGTALVALTALSLP
                     AQASCFWSTAKSYSGAVGLLVGASGLIRTPTGEAVRVGVTAGTAVGELIDYGISHWWD
                     PSLELVPDDPSQVVTDPAQSLIQSAITSLLSDFRGSAPYATTWADLQALNATEAGFGD
                     AMTTMLSSGVSAFVHIAQGEAAYTAFGRGSAQVLAAAARVNDDLQRFSTAQSDLVPYL
                     APFDGTPGFPTVTKASYQSFLDGILSRGAAGLPPGEVSAGQRLLSLAGVHFVPGKALD
                     SSLVTYLSAGDTGNEMAQIPDAGIGLSGLIAARAPGFAGDCCTCGDFCLPEPASVALV
                     LTGVTGLGLQRRRRAIDDLPSAA"
     gene            26049..26822
                     /gene="rfbF"
                     /locus_tag="PKX01_02085"
     CDS             26049..26822
                     /gene="rfbF"
                     /locus_tag="PKX01_02085"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HMZ74938.1"
                     /translation="MKAVILAGGLGTRISEETVSRPKPMITVGGRPILWHIMKIYSSH
                     GIHDFVICCGYMGYVIKEYFANYFLHMSDVTFDMTHNRMEIHERHAEPWRVTLVDTGE
                     STQTGGRLARVRQYVQDEDAFCFTYGDGVADIDIGAEIAFHREHGKLATVAAVQPPGR
                     FGALKIRAGGCVSAFVEKPAGDGGFINGGFFVLSPKVLDAIDGDQTTFEGPVLASLIA
                     RKQLAAFPHTGFWRPMDTLRDKTVLEELWTSGNAPWKTW"
     gene            26807..27874
                     /gene="rfbG"
                     /locus_tag="PKX01_02090"
     CDS             26807..27874
                     /gene="rfbG"
                     /locus_tag="PKX01_02090"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HMZ74939.1"
                     /translation="MENLVNPEFWRGRRVFITGHTGFKGSWLSLWLQALGAEVHGYAL
                     APDTEPALFNVAHIARGMQSTLGDLRDLPALCAALHAARPEIVLHLAAQPLVPRSYAE
                     PVETFAINALGTAHLLEAVRLQPGVQAVVVVSSDKCYENHESLWGHREDAPLGGHDPY
                     SASKACTELIAAAYRRSFLTPRGVALATARAGNVIGGGDWTPTRLVPDILAAFAAGAA
                     VTLRNPEAVRPWQHVLEPLAGYLLLAEKLVLDGLPWAEGWNFGPDDTDSRSVAWIVER
                     LAAGWGDHARWRVADGPRIHETQALRLDCTKARRRLGWHPRWSAETAVDRSLAWYRAW
                     RTGADMRRACLDEITAFGTRP"
     gene            27871..29286
                     /gene="rfbH"
                     /locus_tag="PKX01_02095"
     CDS             27871..29286
                     /gene="rfbH"
                     /locus_tag="PKX01_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HMZ74940.1"
                     /translation="MSVPQTRLAVTDPLAAATALQRRRVQADAAGRIALNLPELAGQD
                     LEVIVRPARAPAEPSAWRPDLDPVPPSGKVIGDAERNAMIDAALDGWLTTGRFNDAFE
                     ARLAAFVGSRYALTVNSGSSANLVAFSALTSPRLGERAIRPGDEVITVAAGFPTTVNP
                     ILQNGAVPVFVDAALPGYNLDVTMLEAALSPRTKAIMIAHTLGNPFDLDAVCAFAERH
                     GLWLVEDCCDALGSTWKGRRVGTFGHLATLSFYPAHHITMGEGGAVLTSDPQLRQIAE
                     SFRDWGRDCWCKPGCDATCGKRFGWQLGSLPTGYDHKYTYSHIGYNLKITDMQAACGL
                     AQLDRLDAFIAARKRNFSYLNAALADAADVLILPEATPGSDPAWFGFPLTLRPEAGVH
                     RVDLLQYLDQHRIGTRLLFAGNLIRQPSMAGRTFRVVGDLPVADRILHDTFWVGVWPG
                     LDTAQLDHIARHIRCFLGVDL"
     gene            29283..30779
                     /locus_tag="PKX01_02100"
     CDS             29283..30779
                     /locus_tag="PKX01_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMZ74941.1"
                     /translation="MNKPLMVFEMANNHMGDLPHGLATIRALREACTGFEDVFDFAFK
                     LQYRDLDTFIHASARGRDDIKYVKRFEETRLSDEAFRALVAEMDACGFRTVCTPFDEP
                     SVDRIEAHGIHTLKIASCSFTDWPLLERMATSAKPVIASTAGATLEEIDNVTSFFLHR
                     GKDFTLMHCVAEYPTPDASLQVNQIELLRSRYPGVRIGYSTHEAPDNTLAVGLALAKG
                     ASVFEKHVVLPTERYAANAYSANPAQIRAWLSEARRALAICGTSGDRYVPSAAERASL
                     QSLRRGVFATCAIPAGTELDATMVEFAFPPVDGQLTANDWSKYARFVTTAPIAAGQPV
                     LQAQVAEHQLRGKVLEIVTAVKALLKAGNVVVPGQSDLEISHHYGLELFHEFGLTMVT
                     VVNREYCKKLLVMLPGQLHPEQYHLKKEETFVVLHGALILWLDGEERHARAGDVITVA
                     RGVRHTFRTEEGAVFEEISSTHDRNDSYYTDPAIAQNTHRKTWLTYWM"
     gene            30785..32617
                     /locus_tag="PKX01_02105"
     CDS             30785..32617
                     /locus_tag="PKX01_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HMZ74942.1"
                     /translation="MPTLRVADWIMGRLADLGVDHVFLLPGGGAMYLDDGLACEPRLT
                     AVPCHHEQAAAIAAEACGRTGAAGNAGFGVCVVTTGPGATNAITPVAGAWIDSVPLLV
                     ISGQAKRPDRLNGRPLRQGGVQEVDVVPMVRSITKYAVTVEAPQAIRAHLEEALFRMQ
                     DGRPGPVWLDIPLDVQAAPVDPDTLPGWTPPAAELPPAPDFATLADLLARAERPLLLA
                     GHGVRLAGAAEAFRQFTERLQIPAVLTWNALDLLPYDHPANIGRPGVVAARAANFAVQ
                     NCDLLVSVGCRLDNIVTAYNPAGFARSAVKVVVDVDPHELAKLPMPVEHPLAVDARDF
                     LDGLLAASADLPPLPCDAWRTRCASWKARYTQNEGRVFPPAGPIGHAHFVEALSDAAP
                     PDTLIATGSSGLAIEFFYAGFRNKAGQRSFLTSGLGAMGYGLPAAIGACLGNGRRPML
                     AVESDGSLQLNVQELATLAGQCLPVCLFILNNGGYASIRNTQRNYFQGRYLGTGPEGG
                     LLMPDLEKLAAVYDLPYLRIDDCAGLADALGRAQALPRPCLIDVHLVPDESLAPKCAA
                     IPRADGSIVSMPLEDMSPLLPLDVLKAEMIVPLLPASLDAPRPA"
     gene            32622..33764
                     /locus_tag="PKX01_02110"
     CDS             32622..33764
                     /locus_tag="PKX01_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ74943.1"
                     /translation="MSTTCRLCGHPLFPEPLLVQHAMPAAAQGLPTAEQLADDQGVTL
                     ALHQCAGCATVQLANPPVPYWREVIRAAGLSAEMRAFRREQFGRWIAEHDLTGRKVLE
                     VGCGRGEYLALLAEAGADAHGLEYAPAAVAACHAAGLHAHRGFLEDPSIRLASGPFDG
                     FAILNFLEHIPAAPLTLQAIAANLTDGASGLVEVPNFDMILRAGLFAEFIPDHLFYFT
                     RQTLTALLERNGFDVLDCRPAWHDYVLSATVRKRRPLDLSPLLACQDALKAAFDAFLA
                     RFAPGRVAIWGAGHQALALISLMALAPRVAYVVDSAPFKQGRYTPATHRPIVAPERLA
                     DGEVDAVIVLAASYSDEVARILRERHGSRFTVAVLRDNRLDTVIAP"
     gene            33761..34363
                     /locus_tag="PKX01_02115"
     CDS             33761..34363
                     /locus_tag="PKX01_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ74944.1"
                     /translation="MTTPSPDALREAIATLRAALGPAPRNGLPEDLFLFVSSLTPLVN
                     VDLLIRDDAGRMLLGWRHDAFYGPGWHVPGGIIRFREDAATRIAAVAARELGARVRFE
                     PEPLCIHEIVNDVREERGHFISMLYACTLDTPPDERLRFDPSAPVDGAWAWHAACPDP
                     LIDAHDLYRPWLGPRGTPRPTRRSGRQPQRHWHLLSGRPA"
     gene            34360..35523
                     /locus_tag="PKX01_02120"
     CDS             34360..35523
                     /locus_tag="PKX01_02120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HMZ74945.1"
                     /translation="MINSLYKNRTAIYFGTGMEDAGLTELARLGARHVLLHYGQGSVT
                     RSGLLDRVMAGLTARQIAVTELGGVQPNPTLPRVREGVALCRARGIDTLLAVGGGSVI
                     DSAKAIAAGAAFDGDVLDLIEGRVRPAAALPLGVILTVPGAGSESSPAAVISDPARSL
                     KLDFAAECLVPALAVLNPENTRGVGLFQSRCGILDAITHVCERYFTQTDYVEVSDRLC
                     EGLIQSLIHYGRAVDGRLDDYGVRAEIMWACKLAHDDVAGVGRKQDWSSHVIANALGG
                     HVDQPHGALTVIVMLGWMKRAAETSPARFVQFGQRIFGLASPTAAEAIAAFEHYIAGI
                     GMPVRLSGLGVDLAPATLDAVVDVCARKNPSGTIGNFVRLGPNDVRTILETAR"
     gene            35537..36646
                     /locus_tag="PKX01_02125"
     CDS             35537..36646
                     /locus_tag="PKX01_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HMZ74946.1"
                     /translation="MSYKIHMDGYAEPRTVFQIEALHAWKHKQYFAPVIVEVSPVGYC
                     NQKCKFCYSDKIETKGDKIPDAILTRLFCDLAGMGVQAVSLQGTGEPLMHGGLPDAIV
                     AGAGKGLRYSLTTTGVVFTEKIQSRILQHLTFMKCSVLENDRDRYALMHGCSPKQWDQ
                     LIANLGAAANRRSREGLDVALMGTVYVELNNFDRLPQIIRFYRDLGLDYIVVQEATYT
                     EFTPSNQSRNTSEHFTAAEIDAMKAEVLKLNTEDFTVKVRFPVDDTSYHVGMYKDCWK
                     DDHCQGIKFFPLVAADGNVYSCYRAWGKSEFSMGSLHTQSFEEIWRGERRQEVERQIM
                     GTPPGGDECAVCGISKLNDILSKYEQPNKWRSFLI"
     gene            36643..37197
                     /locus_tag="PKX01_02130"
     CDS             36643..37197
                     /locus_tag="PKX01_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74947.1"
                     /translation="MSCDLCIKHLPGETDCVWHREHKEHGKDYGPHNILPSNVCPIMY
                     HTLYPYFLGALFGARYAYNEQGDCQVCCPAAKGVDVLVKVRPNDGKFEAGVDADWRDV
                     IHAEVVAVHGDCPYQHKVGDRIVFPTTRSMRTQYMCPAGFNNLFPFLKIDIPSCIDFD
                     RVRCPDWMENIYYRIDENSRVRKD"
     gene            37200..38726
                     /locus_tag="PKX01_02135"
     CDS             37200..38726
                     /locus_tag="PKX01_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74948.1"
                     /translation="MARLALISLHDTTGLVDDARRLIGAGFTLLASRESSSLIAAAGL
                     PVIDLADYTALTEDFGFPPTLHPKLERALTARPADLAEPAIELAYINPYPADVGNDVG
                     GRTILMLAVKGQRLAASSRADLHRVLACLETGGDLPALRRQLADAACFSVARHFAGLI
                     SPDAPFATSFGEAWLDLAEGENPYQRPAVAYRSSDPDPLALSRFRLLGSQPPCFTNAA
                     DADAILHHLCLLAEGFEKNTGTVPWLCIAAKHGNACGIGVSRHAPREAIDRALAGNPR
                     AIWGGEVIVNFPIDGELAAALFASDEREVRLGSRHWMLDVVMAPAFTAEAAELLGRRE
                     FRKLLANPALAAARLPHGRRMLRPVRGGALGQPVADYVLELAACAGNARFDEAAVTDL
                     LIAWSAAYSSNHGGNEVALARGGMLLAAGGGPSTVDAARSAVTRCAEQGHDATGAAFA
                     ADAFFPFTDAPQIVADAGARCGAVPDGSRNDDTVKAFFRERHIAVAWIPAAFRGFCRH
                     "
     gene            38736..39881
                     /locus_tag="PKX01_02140"
     CDS             38736..39881
                     /locus_tag="PKX01_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMZ74949.1"
                     /translation="MNAPDSPLRRLLHTPPATPAAPCRVADVLAGRPVVVYGCGDGLI
                     TFSVFVLDKFGIRPEVCLDAKFTAPTTLGDIPAMAPTDYRPDPALLDRAVAVVTVGKT
                     RFHADIFATLRGLGFTRIVLASDLYEYHLSHAPAGFEQLGQTWFAERADVIERAYALL
                     ADAKSRSVFEAVLQTHVAQMPVPIPRDPLEHQYFPHDVPLAAGVARTLNCGAYDGDTV
                     RQLHARHGRIEALACFEPDLPNFTRLRATLTEHAGQLAGTVMAFPCGVFDDDVQLRFA
                     GGNRINSTLSDDGDTVVQCVALDHVLPDFRPTFINMDIEGAEPHALRGARQLIRANRP
                     DLAICVYHHPAHLWEIALQIHALVPDYRFYLRNYTGFPAETVLYASR"
     gene            39888..40961
                     /locus_tag="PKX01_02145"
     CDS             39888..40961
                     /locus_tag="PKX01_02145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ74950.1"
                     /translation="MTASQRFTAEIDRAVVRFGDGWTALRGARLFITGGTGYFGRWLL
                     PALIRADAVHGLGLTATVLSRDPARFLADAPEVAASPAVRFHPGDVRDFAFPDGDFTH
                     AIHGAATSAAATFHRREDALTKFDTTLQGARRLLDFATERRIPRLLMLGSGAFYGPLP
                     ADRAAYAEDLPVAPSPDNLDVAIGHAKRAAEFLSAAYAERHGLSISTARCFSFVGPHL
                     PLDLHYAIGNFIRDALRGEAITVGGDGTPVRAYLYAGDLVVWLLTLLVRGAPGRAYNV
                     GSDQAVTIADLAQRVARVVAPAVPVRVLGTTTDVATRNVYVPSIERARGELGLDVWTP
                     LDEAIRLTADVARAGAAPTQARN"
     gene            41202..42317
                     /locus_tag="PKX01_02150"
     CDS             41202..42317
                     /locus_tag="PKX01_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ74951.1"
                     /translation="MTTALIPDSGLTAAEAAKRNDLATRKPYVAAKLARIAEMEERGE
                     ISPIIRLEKSYLCNFQCTHCSAEYYMDRHLEKVFKIKDERQKIDLDDIRRLSKEADAL
                     GLARFVITGGEPLVMKDFDAVVEAIDPERHYVITDTNGWFLDLARARHIKSVGVEKVQ
                     LSLDSMVEAEHDAFRNKPGSYKRVMRAIDAAQEAGLNLILSTVLVRGRARTDEFRQMC
                     DFAKARGIGLYVSYAKPTGSCTNHPEFVIDKEDADIVRELEKTHPVFTHMTPSYGSFK
                     GCITVKGIITVTSTAEVTPCPYIDFSLGNLRQTPLKEILDRGMRNPWLGPHRPDCLIG
                     EDPQFIRIHTERTKGAVTLPLPWGQGFSDHDSLQDEQ"
     gene            42314..43108
                     /locus_tag="PKX01_02155"
     CDS             42314..43108
                     /locus_tag="PKX01_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74952.1"
                     /translation="MNTATLAPAAFDDFGRCLPLGVSAPANARSRRYFRLGTPALDPA
                     AIHARLARHLGAGKDMSTDTFIQRIAALRVRLAADPATAGLLDGPAVPFILPRTPVTE
                     IGTLLEEGYLPAVARSFEEAFPNARFTDHAHQGLGGKLSVQPGSRHEILVDALAHGEV
                     VGLYFPCLTEYSLPAAVERMAGLPEHLLLAGGFDTAAAFIAAPDLLLRTDGYPPLLWL
                     AGLQGEKPGIGYHFEAYGYNLTFNRRPHLDQTAEYWWSGVTVLAPA"
     gene            43167..46796
                     /locus_tag="PKX01_02160"
     CDS             43167..46796
                     /locus_tag="PKX01_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ74953.1"
                     /translation="MRSLYPPPRHPYYIVAPDYRRTSAGIRVMHQLCHALRQSGEDAW
                     IQTGVTHPTLDTPRLTDEVRAAHRARGLTPIAIYPEVVAANPLDCAVVVRYILNRPGL
                     LGEQPAYGPDDIYYVYEPELLDIVPGATDVLRLPSINTSLFNNRDNPHDAHRSGSCIY
                     FGRYSAGQQEHPDLADTCTVITQDFPATHEELAELLRRSERVYCFENTAISTEARLCG
                     CPVVMLPSPFFSKEKFFGRGQGFDQGVAFTQSPEDLATAARTVGHIATLYESLEAAFW
                     PALDGLITLTQARADAAPKPDPTTRSSRRPTQDDTEYETWRARQTLQEIDAQILAERM
                     MLTWQQRPGIHLLLALHAGEEHLLADTLDSLAAQLYPEWRLTVATALDMPEGVGDISN
                     LQWLALRDAAHIDYVIDEMAAASPGTWLARIEPGLTLEPHALQVIADYINVRPDWRLI
                     YCDEDTLEADGRYTHPLFKPDFNLDLLLAQAYVGSLIVVEKQAFLAAGRFGTLAGAEN
                     YDLTLRILDHAGEAAIGHLDQLLAHLPRASVRAMQPEAEKAAVQAHLLRRGLDAEVLD
                     GAGYGTRRVICRWAESPLVSIIIPTRDKVEYLRPFIESLEERTRYRPYELIIVDNDSV
                     DPDTLDYLAALAGRHARVRTRILRQPGEFSWSACANAGAAVAEGDFLLFLDNDLHIVQ
                     DDWLDRLVAIAQRPEVGIVAPRLTYPETAKVQQGVWILGMHGTTGNPWDNQLGLTDPG
                     YMGRALCDQNVSAASGSALLVKRAVLTSLNGFDAERFPLLYGALDLCLRATAQGHKIV
                     WTPYAALVHYAGASVVARQRRLEGKLTDLIAAQEANQTLLARWMPRLVSDPAYNRHLS
                     LSFPFKPEHVAPMDWDPHFHDRPRILACPVPGGAGEFRLRAPLRVIGQAGLAQTMICE
                     PPKPFTMRILSPVEVARADPDVLILHQPLEDTQSDALEHYARFLPKVRRILTMDDLVT
                     NLPRKHPLYKSGYKDGRQRLRRVLSFMDRLVVSTQPLADLCADMIDDILVMPNSLEWS
                     LWGDVAPPGRPRTKPRVGWAGAQQHQGDLELIYKVVEALADEVDWIFMGMCPEPIRGF
                     VHEAHGFELDFRAYPRALSRLDLDLAIAPLDVHPFNEAKSNLRLLEYGAMGWPVICTD
                     IYPYQNAPVTRLPNDPDLWIRTIREQIAEPAALKAAGLALQRWVREGYVLENHTARWF
                     AAYGP"
     gene            47091..47939
                     /locus_tag="PKX01_02165"
     CDS             47091..47939
                     /locus_tag="PKX01_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HMZ74954.1"
                     /translation="MAATINTNSMSLNAQRNLSMSQSSLATSVQRLSSGLRVNTSKDD
                     AAGLAIADRMNAQAKGFDVAIRNANDGISMGQTADSAMQAITDNLQRMRELAVQSANG
                     TYTSGDRTNLDAEFQQLNQEIARVVSGTQFNGQNILTFTASFTFQVGAGTASTDQISV
                     TTAGSLASATFGTTTAGALSGSSISGSSSSGATAAISAIDAAIDSLNTSRATWGAVQN
                     RFSSVVQTLSVASENAKSSRGRIMDADFAQETANMTRAQILQQAGTAMVAQANSMPNN
                     VLSLLK"
     gene            48021..48407
                     /locus_tag="PKX01_02170"
     CDS             48021..48407
                     /locus_tag="PKX01_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HMZ74955.1"
                     /translation="MSIQPVGGASTQPYTPPVQSTPGAQGAIRKADAPTAPGDVQAVR
                     DATQNPAAPPTPEQLNQAVKQIQDVIKQTAQSLQFSIDKDSGMTVVKVVDTESKKVIR
                     QIPSEEVMAIAKALDKLQGLLVKQQA"
     gene            48583..49977
                     /gene="fliD"
                     /locus_tag="PKX01_02175"
     CDS             48583..49977
                     /gene="fliD"
                     /locus_tag="PKX01_02175"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HMZ74956.1"
                     /translation="MASISSAGLGSGLDVNSIVTSLMSVEKQPLTQLQTLQNKDQSKI
                     SAIGTLKSNLATLQSAAAALTPAIGQSPTAAFAAYTATLADTTIGSATATASAVAGTY
                     SVNVTSLATNQQLALGKTYASTDPVIDFGSASTKTLTFTVGSTATNVTLNSSANTLAG
                     VRDAINSANAGVGATIVTGTDGKQNLLLSATNGGTANAVTISGDASFIDASAPGSPIA
                     AASAFTQTQAATDAVVKIQGVSIATSGNTVTNAIDGVTLNLTKTGSTTLTVARNNSDL
                     QGKVTAFVNAYNSLNSSIKSLGAYDATTKSAAILNGDSSLRSIQSQVRAALTGTPTLS
                     SGNANIKLLSDIGVSFQRDGSLAFDTAKFATAASKDYVATATVIANYGSALKTTTTNL
                     LGTNGVIASRTNGLNASIKSIGNQIDSMNVRLTRIEANYRSQFTALDTAMSSMTTTST
                     YLTQQLTLLANQSK"
     gene            50017..50427
                     /gene="fliS"
                     /locus_tag="PKX01_02180"
     CDS             50017..50427
                     /gene="fliS"
                     /locus_tag="PKX01_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HMZ74957.1"
                     /translation="MSFGLAARRADAYASVGVETGVSTADPHQLILMLFDGALLQVGA
                     AAAAMESGNVPGKGAAVSKAIEIITNGLKVSLDYEAGGELAARLGALYDYMTHRLLYA
                     NLHNSRPALDEVTKLLSELKSAWAEIGPGTDGSM"
     gene            50535..50873
                     /locus_tag="PKX01_02185"
     CDS             50535..50873
                     /locus_tag="PKX01_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HMZ74958.1"
                     /translation="MSSLALLEEMSTYSNEMVDAARHNDWDRLTRLEKKVASLRDRIG
                     VEEALGSPSRRRMSESERSRRIDLIRRILDDDKEIRVHTEPWLDNVRQMLTGQSRERA
                     VRAAYDKLDR"
     gene            50928..51974
                     /locus_tag="PKX01_02190"
     CDS             50928..51974
                     /locus_tag="PKX01_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HMZ74959.1"
                     /translation="MIPSDLAARLRMLTEASFFSGEPTVAPLARVREVPANLPDFLPG
                     QRIVARLQQSGPDNTFKALVEGRSVTLALPQTATPGDTLELVVSHVTPAAVFATQAGS
                     TALPGAQTGTALSQAGRLISFLLTGQPPAQAASLNGGQPVLASPPGPGDTDLLARRLG
                     AALGDSGLFYESHQARWLAGQVDMAQLTREPQGRPAAPSAAPTTALQTGTAALPEAAQ
                     PGATTQIPDRLLPVVHQQLDALATQHLVWQGQIWPGQTMEWEIEDPARDGPRADDDPA
                     PTWNTTLRLSLPTLGGVEARLHLTPAGVALRLTGDSADTVAALRAGQPRLAEALAAAN
                     LPLTGVSTEQREPA"
     gene            51971..52291
                     /locus_tag="PKX01_02195"
     CDS             51971..52291
                     /locus_tag="PKX01_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HMZ74960.1"
                     /translation="MNGPAQPEIPRAEAVALAYNAGNGAPSVVAKGRGLIAGEIIARA
                     REAGVYVHESPELVALLMQVDLDARIPPELYVAVAELLAWLYSLEDHARHQKETAPQG
                     RPLP"
     gene            complement(52302..52574)
                     /locus_tag="PKX01_02200"
     CDS             complement(52302..52574)
                     /locus_tag="PKX01_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HMZ74961.1"
                     /translation="MVRMVNCVKLGREAEGLDMPPLPGDLGKRVYENVSKEGWQQWVR
                     HQTMLINENRLNLMDTRARKYLAEQMERYFFAGGADQIGGYVPPKP"
     gene            complement(52635..54296)
                     /locus_tag="PKX01_02205"
     CDS             complement(52635..54296)
                     /locus_tag="PKX01_02205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ74962.1"
                     /translation="MSAICSRICALRWRQVGAAVVPSLWAGGATAAEAWPGGSLPALA
                     VVPALAAAALWACVLWRRIRALEATRLDPPVPVEPASRTGLPVASPAAGPWEHYFALA
                     ADSGHVLEGLIDTRGRLYWVSPAFERFAGHAVEDVLAAPDFVELIIYERDRRYVREQT
                     TQVLSTGRPADFETRVLTREGALRWVTCHWHAVRDEAGVVQGIRATGDDIQARKEAEF
                     KLLETVAALRRAQALSEHYLRRSNEERSRLSALLDVVEAGILFMDPDHRVVYCNRALC
                     EIWKVAEGESIVGVRDVVLQQHAVTLLTDPPGYLRHVAGVLGSREASAPFELHFSDGR
                     TVNEFSRVVFGDDGARAIGRIWVYEDVTERRRVALQLLNLAERDPLTNLYNRRRFHEE
                     LERMLGDARRRGDDVGLLVLDLDGFKPVNDAFGHQAGDQVLTGLAQAVAAVVRRNEMF
                     FRLGGDEFAILVPSADVQGLAGLARRVGACIAALRFDFGGSAVGVTASIGIAWSGLSG
                     TDAEALVGAADRAMYRAKAQGGNRSEVAAAAEGNDPEVGAEPGLG"
     gene            complement(54311..55663)
                     /gene="argA"
                     /locus_tag="PKX01_02210"
     CDS             complement(54311..55663)
                     /gene="argA"
                     /locus_tag="PKX01_02210"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HMZ74963.1"
                     /translation="MTVPQSVPAAVPDPGLSQRFVAWVRAAAPYIHAFRGKTFVLGFG
                     GEVAAGSLAQTLAYDCNLLAALGVRLVLVHGARPQIEAELARRGLQPRYHKGLRVTDA
                     DAIECVKAAMAVTRIEVEALLSQGLPNTPMAGSFMRVTGGNFVTAKPVGVVDGVDYGF
                     TGAVRKVIAEEIEADLDQQNVVLMSPLGVSPAGEIFNMSMEAVAEAVAIALKAEKLIY
                     LCDAPGLLDEGGGLIEAITADDAEAMLKAGKGLTEDLDLYLPCAIRAVRKGVGRAHLI
                     DRDKDGGLLLEFFTPQGVGTVLTRDPLFRLREATIEDVGALGSMIAPMEADGTLVRRS
                     RERLEQEIERFTVVEYDGVLVGCAALYPFIDDRAAEMACVAVVPEHRRAGLGEMLLKR
                     IEARARALGVERLFVLTTRTSDWFRERGFRAMPPEELPRQKREMYDVERRSKVFMKAL
                     "
     gene            complement(55745..57232)
                     /gene="pap"
                     /locus_tag="PKX01_02215"
     CDS             complement(55745..57232)
                     /gene="pap"
                     /locus_tag="PKX01_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HMZ74964.1"
                     /translation="MFESAELGHKLSKAEYAEIEPVLRTELLETQLDLLDKKDFAAVV
                     LINGIDGAGRGETVNLLNEWMDPRHIEVHAFDRPTDEERERPFLWRFWRALPPRGKIG
                     ILFNNWYSEPLGDRAFGRIKKARLDQRLSEFNRFEAMLAAEGVVLVKFWFHLSRDGQK
                     KRLKALEANPATRWRVRASDRLVYDHYDRYRATAEHVLRRTSTAHSPWVIVDGSDPQY
                     RAVFVGRTLLHRLQAHLEHVRKGYRARLVSAPLVAAADRRTLLNALALDQPMERKAYE
                     AQREALQGRLSLALRRRGFTQRSLVLVFEGMDAAGKGGAIRRVTQALDTRQYHVIPIA
                     APSDEERAQPYLWRFWRRLPRRGRVAIFDRSWYGRVLVERVEGFCTEADWMRAYAEIN
                     DFEDQLVSHGALVVKFWLSISADEQLRRFKAREEAAFKRYKITPDDWRNREKWPLYEP
                     AVCDMIDRTSTENAPWTLVEADNKYFARLKILRTIVETVERSLKA"
     gene            complement(57234..57965)
                     /locus_tag="PKX01_02220"
     CDS             complement(57234..57965)
                     /locus_tag="PKX01_02220"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HMZ74965.1"
                     /translation="MNPRFFCPDGLQPDSEMILPAAVAHHAERVLRLAVGEGVTLFDG
                     RGGECDAVLVALGRQPLARLGPRRAVERESPLAITLVQALAAGDKMDWVVQKAVELGV
                     AAIQPVAAERSVLKLAGERAAKRVAHWQQIVVSACEQSGRNRVPEVAEILPLARWLGT
                     PFAGRRLILAPGDAGALVRMAPPDGPLAVLVGPEGGWSPAELGLAMRAGCEPLALGPR
                     VFRSETAGLAALAALQTLWGDFPGG"
     gene            58017..58580
                     /locus_tag="PKX01_02225"
     CDS             58017..58580
                     /locus_tag="PKX01_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ74966.1"
                     /translation="MVSLSPPVCDFGWSAPDFALPGVDGRTHSLASCRGPAGLLVMFI
                     CNHCPYVKAILPRLLRDCRELAGLGVGSVALMANDPADYPEDSWEAMQRIAVEQDFPF
                     PYLLDASQDVARAYGAVCTPDFFGFNRDLKLQYRGRLDASGRQPAPSDARRELFEAMA
                     QVARSGEGPRDQTASIGCSIKWRPSAP"
     gene            58634..59383
                     /locus_tag="PKX01_02230"
     CDS             58634..59383
                     /locus_tag="PKX01_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HMZ74967.1"
                     /translation="MARDVILPRYLRAVREHKADGSVLTEADLAAQAALEERLPHLIA
                     APVLGEEMTAEEQGRVWHAGKAGLWVIDPIDGTTNFANGIPFFGVAVAYLVDHKTQLG
                     VVYNPVTDEAFYAGRGAGAWLNDVSLPLRQPTERLKDAVAGVDFKRISHHLGDELAVR
                     PPYYSQRNFGSSALEWCYVAAGRLDVYIHGGQMLWDYAAGRLILEEAGGCYSSLGGTP
                     LTAGPAIKRSVISAASPALFEQWRTWLHAHA"
     gene            59472..60347
                     /locus_tag="PKX01_02235"
     CDS             59472..60347
                     /locus_tag="PKX01_02235"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HMZ74968.1"
                     /translation="MSMKHPIIVVTGSSGAGTTTVRHTFEAIFWREKVNAAIIEGDSY
                     HRYTREEISALITDAENRGERGISHFGPEANLFEELENLFRTYGESGSGQRRHYLHSV
                     EEAARRGLKVGSFTPWEDLPPDTDCLFYEGLHGAVVTDKVNTCRHADLLIGVAPITNL
                     EWIQKIHRDTRTRGYSMEAVQDTILRRMHDYVHYIVPQFTHTHINFQRVPMVDTSNPF
                     IARDIPTADESMVIIRFRDPRGVDFPYLLRMIHDSFMSRANTIVVPGGKLDLAMQLII
                     TPLIWKLMERRRQWV"
     gene            complement(60363..62141)
                     /locus_tag="PKX01_02240"
     CDS             complement(60363..62141)
                     /locus_tag="PKX01_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74969.1"
                     /translation="MTRLTPFAFAMLVGGSLAQAQSMPAADEPSAAAPADACPILLQG
                     AEAALERGRAGIAARAWKDAAAVLREASASFLKVAAGCPALAPQANRQGEKAVAELKL
                     AEGQMSHHSECQPRLDKALDLDIRAASVKSASGDPAEMERLLGESEALWREAVSLCLS
                     PHREKAERSLAATVRARAANAELLSAGPACDGAWKNATTLVDLAKAAWKDKRWDDAGM
                     LYSKAALAWEGAADKCSGNRQQLAQRKIEQTQTDAHNAEFCGPLWDAATEQSQRVKAA
                     GTSAALAERDQLSIRAEVAWRDAAAQCRGAPQNLARSNADALARERGAPLPPNAMAQY
                     GRKLPVAGSAAAVAPASVPVAGAAGATAQVASLAVTPPLPTRPAPRDEPARPVVVAPP
                     PAKPDTRSEPVAAKPAAAPAPAEPTVIVAGDTTYRGAFVADQAAAGAVSGSGSVEWAN
                     GERYTGSLVGGRKQGRGRFDWVGGQWYEGEWQDDRATGQGVIQFVGGNRYEGGVVDGE
                     PQGRGTLIFASGDRYTGDFVRGVFHGQGTYAWKNGNRYEGAWNLGRKHGPGRLMWAVG
                     GGWEGEFRDDQKTDAGRDITVAAAPR"
     gene            complement(62161..>63564)
                     /locus_tag="PKX01_02245"
     CDS             complement(62161..>63564)
                     /locus_tag="PKX01_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74970.1"
                     /translation="FCDLRAMAAFPRGRRPLLALIAAALLAGGCSTTKYTVDDGRKVN
                     EALLANLRLLGSGERALRPAIVRSAELKDPDCSHQWDLPVSVATSYEWPEDDRVAWVR
                     ALQVDERLTVVAAAPGSGFQVGDKIVEIDGYKREDSNKMLVELAEMRDNGKPFPVKTA
                     AGRTVTLKPVQVCRGYTRLAPPATPGYQDFHWLMSIHPLQVFIPPVTPDEALWMVLWT
                     QGLSEEGGARMKTYHYSKEIVSTLFEIASLATGLNAAAQAAKVAVNQAATAAATAAAK
                     SASEAAARQALEQAARQISQQAAKDYAQKVGEEVAKSVGRQGVNVLRDSFMARMGMAV
                     SSLSWVATTVFDDADRWAYDRIAKLGGDPLAGATLHQKLLDKGLIQNAFVLEEERLVR
                     YAALARQTQREEMLAAVLKGASLEAFTLKLTDLPESSSEPAALEMASEMAEQGPGGTS
                     GGLIDALLRMPLESGDD"
BASE COUNT        10235 a        24372 c        19619 g         9338 t
ORIGIN      
        1 cgatggacgg cgaataggcc gcaccgcagc agatccgcag gaaatggtcg aaacgcccgg
       61 aattggaaaa catcgcgccg ggcgccacgc ggatgccgct gcgcagcgcc gcatcgaaga
      121 ccgccatcga atcgcacccg gccggcagct cgacccacag caacatcccg ccgcccggcg
      181 gattgaggcg cgtgcccgcc ggaaaatggg tggcgatggc ctctgccatg tgcgtccgct
      241 gcgccagaag atgccggcgc aggcgtacca ggtgccggtc ataggcgctg ccggccatga
      301 actcgccgat ggccagctgg gccagggcgt cattgggacg gctctgggcg tgcttgagca
      361 tgcggatgcg atccagccag cgcccgccca gcatccagcc cacccgcatg ccgggcgcca
      421 gggtcttgtg gaaggacgcg cagtggatca cgctgccatc ccggtcccag gccttgcacg
      481 ccttcaggct ctgatcgtcg tcgtcgaggg cgccgtagat gtcgtcctcg atcagcgcta
      541 cgccggcgcc cgaacagagt tccaccaggc gcgccttctc gccgtccggc atcacgcagc
      601 ccaggggatt ctggaaattc ggcaccacca ccaccgcacg cagatccgga tgcgtccgga
      661 tggccagctc cagcgcctcc accgacaggc cgcgactggg actggtggga atctccaggg
      721 cacgcaagcc caggctctcg atgacctgca ggagcccgaa ataggcgggc gactctaccg
      781 cgatcacgtc accgggcgct gccacggcgc gcagggccag attcagcgcc tcggtgcatc
      841 cgtgcgtcac caggatctcg tcgggcgccg gactcatacc cgcggccacg gcccgccgcg
      901 cgagcacgct gcgaaagccc gcgtccccca ggggcggcac cggattgacc agcacctccg
      961 gatggcgccg cagggcacgg atcgcggcgt tgcgcagggc ctccaggggg tacacctccg
     1021 gcgggccaaa ggcctgggcc agattggtgt gggtcgggaa ccgttcgcac ttggcgacga
     1081 aatcggagac cctggcgtgg atcccggcaa agacggccgg atcggggagg ctcggaggac
     1141 gcccgacgtc cggctcacgc accggctgca gggtgctgcc acgtgccgca cggacaaaat
     1201 agccggaccg tgggcgggct tccagcagac cttcgtcctc caggcagtgg caggcctgca
     1261 ccaccgtgga cagactgacc ccgtgcagac gggtcagcgc ccgcagggag ggcagacggt
     1321 ctcccgcggc cagggcgccg ctgcggatgg cccggcgata gtgccccgcg acacgcaggt
     1381 agagggtttc ggcagtgtcg atacggtcca tgcgcgatct tcgcccgacc cggcggtccg
     1441 gcacaggtac agattgacgg aaagcggacc atgacagcaa gcaattcagc cacctgaacc
     1501 ggtacacaaa gcggcgtcct gtgcctgccg gtggacgcgc ccggtcctta agctgtaccc
     1561 gtcactttcc ggagaacctg ccatggacgc ccctgccccg atccgcctgc gcggcggtca
     1621 acgccaccat gcccacctgc ccgcgggcac cctcatcatc gcctgcgccg ggcgcctgag
     1681 cgtcaccgat gcgcctcgct ggctcggtga ccaggtcttc caggtgcacc atcgccttgc
     1741 cgaaggcgat gtcctgcgca tcgaagcggg cggctggatc gcggtgagcg ccgatggtga
     1801 tgccagcttc gtctgcgcgc cgccgccagg cctgacgcta cgtctgcacg acggcgccgc
     1861 gcaactcgaa gcgtggttcg tccagacctg gcgcgcccag gtcgaatccc ccctggcact
     1921 gctgcgcgcc cgcctgcgct ggctccagac gctgcagcgg cgtcggctgc gcgtggaagt
     1981 gtccgcactg cgggctcgac tcgaacgctg aacccagggc gctggacgcc cctcatccgt
     2041 agcccggcat gggcgcgccg cggacacacg gcacccgggc gagcgaccag atgccctgcg
     2101 cccaggccag gagttcggcc ggtgctgcgc cgccgagcgc cgcgggtggc ggatgcccga
     2161 ggaaacgcag ggcatccgcc accacacctc ctggcgccag gccatccacg gcctgggcga
     2221 gggtctgctt ggagagcttc tcgcccgcgc cattggtcac caccggaacg tgggcataag
     2281 ccggctgcgg gagtccgagg gctcgctgca gcaggagctg acgcggggtg gaatccagca
     2341 ggtcggcgcc ccgcaccacg tcggtgatgc ccgcctccgc gtcatccacc accaccgcca
     2401 gctgataggc gaaatatccg tcggcccgca gcaccacgaa gtcacccaca tcggccgcca
     2461 catcctcctc aatgggcccc tggatccggt cgacaaagcg cacgcagccg ggctccaccc
     2521 tgaggcggaa ggcccgagcg gtccggccgg gtggcagacc atcgcgacac gttcccgagt
     2581 agcgtcgcgt cccgtcccgc gccaggcatg cgtccgccag cgcccgccgt gtgcagatgc
     2641 agccgaagac ccgtccggcg gccatcaggc gctccagcgc ggcccggtag acctccgtcc
     2701 ggctgctctg ccagagcacc ggcccatccc actccagccc gagccgttcg agggtccgca
     2761 ggatcccctc ggcggcaccc ggcacgcacc tcgggacatc cacgtcttcg atccggagca
     2821 gccacgccgc ccctgaggaa cgggcatcga gataggaccc gacggcagcc accaccgagc
     2881 cgaaatgcaa gggacccgtc ggcgagggcg caaaccgccc tttccgggcg gcccctccgg
     2941 gagcgctcac agcacacccc gcacgcccgg aaacttcttt tcaatcatgg aaatacaccc
     3001 acacacaagc cattattgct tatgacatga aatttaacta ttactttcga catattttcc
     3061 tcattcccca aagccgcgat aattatcggg tgtctttttc cgtcatgcag catgacggat
     3121 tcaggccggt ccccggccgc cccctttcgc caggaagcac ccctcgccat caggccccgc
     3181 ccccatgcca gacgtccatc gcgtgaagct tgccggtctc ttcgctcccg ctccggccgc
     3241 ctgcacggcg gccggccccc gcgatgtccg cctccccgga cgataccccg tccgcatggt
     3301 tgcagccgga ctgctcgcgt gcctgatcgc gttggtggcc ctgtggccga cggtggatgc
     3361 gccacgcccc ctgatcgtct ggtgggcgtt ggtcacggcc ggccagctcg gcctcgccag
     3421 cattgcccac cgggtgcgga ccggcagcgg cccctccgcg ctggatgaag acctgggcca
     3481 ttacctcctg tccttcggcg ccgtagtcgc cggcgcggcc tggggcgccc tgcccctgct
     3541 cggcccctac acctccggaa caccggccca gctcaccctt ttcctcctgc tcggcggaat
     3601 cacccttggc ggcgcagcca tcctgtcggc gagccgcagc gccttcgccg tgttcgtgct
     3661 ggccacgctc ctgccgcttt tcatcctcac ggtagacctg ccgcccccga acatgcccca
     3721 tgcgagcacc gggctcatcc tctttggcgc cttcgccttc gcggtacacg atctgatcca
     3781 cgccgcgcac cggccgcgga gcagtccgcc cgctgccgcc gccgtcggcg gcttcgagga
     3841 aaagatcctc gatggtgccg gtgaagccat cgtgctgtgc accggatcgc gcatcatcca
     3901 tcacaaccgc cgcttcatcg aactggtggg cgagcgctcg acccatgccc gcgccgaagg
     3961 tcgcctggcc cactggctgg ccgactcctc cgagcggcgg cgcgcccgca cggccctgag
     4021 cgccctctgc cagggacggg tctaccggga cgtggtccgc ctgcgccgcc gtgacggcag
     4081 catcttcacc gcagaggtct cgggtcgggc cctcgatccg cacgcgcgcc ccctgcgcgt
     4141 ggtgtgggtc gccaccgaca tcaccgagcg cgtggccggc gaggtccgcc ggaccatgct
     4201 cggcacacag ctccaggaac tggtcagcca gagtggcgac ggctactggc agaccgacgc
     4261 gctgcaccgg ctggtgcacg tccacgcctg ccacgcgcca ctggacggca tcctcgacgc
     4321 ccgcatcggg cgtccgtggt ggacgcagga cccccgcccc ggcgtccagt ccgatgcgcg
     4381 acagaccgag atccgccaga tcttcgagga ccgcggcgcg ttccgggacc tcatggtgga
     4441 ggtccgcgat ggctatcacc ccccccgctg gctgacgctc agcggcacgc cgcggcagga
     4501 tgaacacggc tgcttcctcg gttatcacgg catcgcggtc gatgtcaccg aacgggtccg
     4561 gggcaccgag cgcatccgtc atctggccta ccatgatgcg ctcaccggac tccccaatcg
     4621 acggctcctc gtggaccggc tgggccaggc cattgagcgc gcccaacgtt atcgcgagcg
     4681 cctgggcgtg atcctcatcg atctggatga cttcaagcgg atcaatgatc tgtcgggcca
     4741 ttccgtcgga gaccgggtcc tgctcgacac ggcggaccgg ctgcgggcct gcatccgccc
     4801 ctgcgacacc gtcgcacgcc tgggcagtga cgagttcgtg gtcctgctgt ccgaactgga
     4861 tcagccctcg gacgccgacc agatggcggg cacgatcctc cacgcactcg gccagcccgt
     4921 cgccggccgc ctcggcagtg cgccgctggg caccagcatc ggcattgccc tctatcccga
     4981 ggacgcccag accgccgaca gcctgctgga ggtcgccgac cggcgcatgt accgcgccaa
     5041 gcgccgcggt ggccagacca tcgaacatgt ctgaccggct ccatccagcc ggtcccgcgc
     5101 ccatgaaaaa ggccgggcgc cctcaggcga cccggccccg atcagtgccg cacgccgttt
     5161 attcggacgt ggcgcgacgg atgcgacgct gacgcacgcc ttcggccagc atctccagca
     5221 cggtcacgct gtcctcccag ccgatgcacg cgtcggtgat gctctgcccg tactccagcg
     5281 gcacccccgg cttcaggtcc tgacggcctt ccttgaggtg ggactccacc atcacgccga
     5341 tgatgcggtc ctcgccctcg gccagctgcc cggccacgtc gcgggccacc tccacctgca
     5401 gcttgtgctg cttgcggctg ttggcgtggg agaaatccac catcaggcgg ccctgcacgc
     5461 cggacgcggc cagctccgtg caggccgcat cgacgctggc ggcatcatag ttggggccct
     5521 tgccgccacg caggatcagg tggcagtcct cgttgccgga cgtggacacg atggccgagt
     5581 gccccgcctt ggtgacggac aggaaatgat ggggcgactg ggccgccttg atggcgtcca
     5641 cggcgatgcg gatgttgcca tcggtgccgt tcttgaagcc caccgggcag gacagtccgg
     5701 aggcgagctc acggtggacc tgactctcgg tggtgcgcgc tccgatggcg ccccaggcga
     5761 tgaagtcggc gatgtactgg ggcgtgatca tgtccagaaa ctcggtgccg gccggcagac
     5821 ccagctcgtt cacctcccac agcaggcgcc gcgccaggcg caggccttcg ttgatggcga
     5881 aactgccgtc cagatgcgga tcgttgatca ggcccttcca gcccaccgtg gtgcgcggct
     5941 tctcgaagta cacccgcatc accaccagca gatcgttctt gagacggtcc gcctcggcct
     6001 tgagacgccg ggcgtagtcc atcgctgcct cgtaatcgtg gatggagcac gggccgatca
     6061 ccaccagcag gcggtcgtcg gcgccgaaga ggatgcgatg gatcgcgctc cgtgccgcaa
     6121 acgtggtgtc cgcggccatg ggcgtggcgg ggaattcccg cagcacatgg gcggggggcg
     6181 ccagttcctt gatctccttg atgcggacat cgtcgatatg gactttcgcg gaggcatgca
     6241 tggtgaatcc tgtcagctac ggggagagcc ggtgatttta gcccatggtc gtccgtgaag
     6301 gccatggccg ccggtgcgcg gccacgaccc gcccgggcgg cgggcaccct agagcatcac
     6361 ctgggtgccc agttccacga cctgattggc cggcagatgg aaggacgaac cggccccttc
     6421 ggactggcgc accatccacg cgaacagcgc acagcgccag cgcggcagct cgcccggacc
     6481 gtccaccacg ttggagcggg ccacgaagaa cgtggtcgtc atgggctcca gctccaggtc
     6541 gtaacgggct gccagggcca gtgccgccgg cacgtcgggc tcctcgcgga agccgtactt
     6601 ggcctccacg ctgaagatgg acggcgccag ctgggtcacc tccacccgct cctcgtcgtc
     6661 gatgcgtggc acatcctggt tggccacatg gaggaaaacg atgcgctcgt gcagcacctt
     6721 gtagtgcttg aggttgtgga acagggcgct gggcaccaga tcggaatcgg acagcagata
     6781 caccgccgtg cccgccaccc gcggcacatc cggcagcggc cccttgatga agccggccat
     6841 gggcacgtcg atctggcagc gcttgcgggc cagcagcaga ctgccctgct tccaggtggt
     6901 gagcatcagg aagatcgtgc cacccagcac catgggcagc cagccgccct cggcgatctt
     6961 ggtcaggttg gaaccgaaga acatcagctc cagcaggccg aacagcagca gcaccgccag
     7021 cagccccacc cgcgaacgcg tcggcgacat ccacacgatg aaggcggtga gcagcgtggt
     7081 gatgatcatc gtgccggata cggcgatgcc gtaagccgcc gccagcgcgc cggaggagcg
     7141 gaactggaac accagcacga gcaccgccac cagcatcagc cagttcacgc tgggtacata
     7201 gatctggccg cgctcggtat cggaggtgtg gaggatgcgc agacgcggca ggtaacccag
     7261 acgggtggcc tgcagggtca tggaataggc gccggtgatg gtggcctggg acgcgatcac
     7321 cgtggccagg gtggcgagcc cgaccaaggg cagcaggaac cagtcgggcg cgagcatgta
     7381 gaagggattg tcgatggcca ccggctcacg caggacaagt gccccctggc cgaagtagtt
     7441 gagcatcagc gagggccaca ccaggccata ccaggccagg cgcaccggcc gtgcgccaaa
     7501 gtggcccatg tccgcgtaaa gtgcttcgcc gccggtgagg gccaggaaca ccgccgacag
     7561 cagcacgaag gcgaccttgg gcttttcaaa cgcgaattcg acggccaggc gcggatcgat
     7621 ggcctgcagc acgcggggcg tctccatgat gctggcgaca cccagtaccg ccagggtcag
     7681 gaaccacacc accgtgaccg gcccgaagaa acgccccacg tggcctgtcc cgtgtttctg
     7741 gatcaggaac agcgccacca ggatgcccac cgtgatgggc accaccgcgt gctccaggtg
     7801 cggggtggcc acgctgatgc cctccaccgc agacagcacc gagatcgccg gcgtgatgat
     7861 cgcgtcgcca tagaacaagg ccgcggcgaa gatcccgcca gccaccgcca gcacactcca
     7921 gcgcggattg ctcgacaccc gatgggcaaa ggccgtcagg gccagcaccc cgccctcccc
     7981 ctcgttgtcg aacctcagca cgatgcccac gtatttcacc gagacgatca gcgccaccgc
     8041 ccagaaaaag gccgacaggg ccgccagcac attgccctcc gaggggtcga tcccgtgcgg
     8101 tccggaaaac gcttccttga acgcgtacag cggactcgtg ccgatgtcgc catagacgac
     8161 ccccagcgcc gcgaggctca gggccagcag gcccgccctg gaatgactgg atggatcact
     8221 catcgataac tccctgtggt cccggcatgg cggccggtgt tcgtcgaagc ctatacctga
     8281 aaccggtaac ccaccccggt ttcagtcaag aaatggcgcg gctgcgccgg gtccgcctcc
     8341 agcttgtggc gcagatgccc cacgtagatg cgcaggtagt ggctgctgtc gctgtacgat
     8401 ggcccccaca cctcgcgcag cagctggcgg tgggtcatca ccttgtcggc attggccagc
     8461 agcaccgtca gcagacggta ttcgaggggc gtcagacgca ccggctctcc ctggcggatc
     8521 acccggcggc gcgacagatc cacctccacg tcgccgaaga cctgcaccgc ggagacctgc
     8581 tcatgggccc gcccgcggcg cagcagggcg cgcacccgcg ccttcagttc gcccaccgaa
     8641 aagggcttgg tcaggtagtc gtcggcgccc gcatcgagga cgccgatctt gtcgtgctcc
     8701 gtcgagcggg ccgacagcac cagcaccggc gcggccgacc aggtccggaa gtcctgcacg
     8761 aaacgcgtcc cgtcgccatc cggcagaccc agatcgagga tcaccaggtc cggcgcatgg
     8821 cctcccgcct ccaccaggcc ctgcccgcag gtgtccgcct cccgcacgac gcagccgtcc
     8881 gcctccagcg ccgtgcgcac gaaacggcgg atctggtgtt cgtcttcgac gatgaggatc
     8941 tgggctgcgc tcaaagcatc tctcccgagc cttctgcgac gggctcgtgc tctaccggcg
     9001 gcggcgtgcc caagggcagg cgcagcgcga tgcacccccc gccccccggc ggattgaacg
     9061 cacggatcgt gccgccgtgg gcttccacga tgctgcggca gatggccagt cccagaccga
     9121 ctcccggcac cgcggcctcg ggcgtgccgc gctcgaacag ttcgaacacc tgctcgagcc
     9181 ggtccggggg aaaacccggc ccgccgttgc ccacgcggat ctccagcgta tccgggccgg
     9241 cgcgggccgt caccgtcagc ggcgacccct ggggtgaata tttgacggcg ttctcgaaga
     9301 ggttgcagaa cacccgctcc atcagcaccg catcgcactg caccagcggc agatccccca
     9361 gcccggcggt gcgcaccgga tggccggcca gggcgccgcc gagggatttg aggctcgcgc
     9421 ccaccacctc ctccaccgac tgccattcac tgcgcagcac caccttgccc gcctgcaatc
     9481 gggccatgtc cagcagattg accaccatgc cattcaccct cagggcctga tcgcggatcg
     9541 cctgcgctgt ctccagcgcc tcgccggaca agggcgggcg ggtcagcagc agggtgtccg
     9601 ccaatccgta cagggccgtc agcggcgtgc gcacgtcgtg ggacagggcc gacaggatcg
     9661 agctgcgcag ccgttccgtg accgcctcca gctgcgccgc ctgggccgcg gcaacgaaat
     9721 gcaggcgctc gacagccgtc gccgccagcg acgccaccgc ctccaccagg ggcagatcgc
     9781 ccaccggtcc gccggtcggc agcgacacca ccagcacgcc acgcgcccgc gacgcccccg
     9841 ccagcggcag ataatggcgg cgcagacccg gcacctcggt cgcccccgac tcgatggcac
     9901 acccaccgtc gtacgccatc cgcgcggtgt ggccgtccag ttcaccgaaa gggccgtcct
     9961 cagccttgac caccgccagc acatccgcgg cgtcgggcag caccaaccac atccgcgcgt
    10021 ccacgtgggc atgcaggaat gcccgcagcg ccgcctccac ctcgaccggc tcgcgagccc
    10081 cggcgagaga tttggccagc ccgtagaggg ctcgcgtggt gcgttcgcgc tggccggcct
    10141 cccccgcctg aagacgcaac ccggcggtga gctggccgac gatcagcgat accgccagca
    10201 tcaccgcgaa gctggccaga tactggctgt gattgaccgc aaacgagaac agcggcggca
    10261 cgaagaagaa atcgaacagg gccacgctgc ccacggctgc cagcaccgcc ggtccccgcc
    10321 ccagccgcgc cgccacaagt accacggcga gcaggaagag catctcggtg ttggccaggt
    10381 ccagcacgtc ccgcaagggc aaagccagcc ccgccgccag tccgcagacc agcactgcca
    10441 gcccgtaacg acggacggca ggcgcttcat cgcgctggta aaagggcatg gtggcggaca
    10501 tggggcgcag gaggcctcgc aaaacgcgtc ggggccgatt gttgcaccgc ctccataaaa
    10561 atggcgtaaa tgatctgcgg cgactgtggg cgagtgtata gtgtattttt gtacactaaa
    10621 ccgcatgaaa cggccgatcc cccttcaggg ccgcggcagc cggctcaatc ctgacacccg
    10681 ttttgccgac tggcagcgcg cagccttcga cgacggctgg gacgccgccg gcgacgcgcc
    10741 ggtgccccca ccgaccgtcc tgatggtgga cacggcgcgc tcggtcatct gccgcaatac
    10801 gtcgcccgac gtccccttca gccagtccat caatccctac aagggctgcg agcacggttg
    10861 cagctactgc ttcgcgcggc ccggccacgc ctatctcggg ctctcgccgg gcctcgattt
    10921 cgagacccac atcgcctgga aacccgatgc cgccgccgtg ttgcggcgcg aactggcggc
    10981 gccgggctac cgctgcgccc ccatcgccct gggcatcaat accgacggct ggcagcccct
    11041 ggagcgtcgc ctgggcctgt cgcgtcagct tctccaggtg ctggcggaga cccgccatcc
    11101 ggtgtccatc gtcaccaagt cggccctcat cgaacgggat gcggatctgc tcgccgatct
    11161 ggcacgggac gatctggtgc aggtgatgtt ttccatcacc accctggacc cggatctcgc
    11221 ccggcgcatg gaaccccgcg ccgcgtcgcc agcgcgccgt ctcgccgcca tggactgcct
    11281 gcgccgggca ggcgtgccgg tgggcgtgct gtgcgcgccg ctgattccgg cgctgaacga
    11341 tcacgagctg gaaggggtgc tcgctgccgc caaaggggcc ggcgcagaaa ccgccggcta
    11401 cgtgctgctg cgcctgccgc gcgaactgca ggcgctgttc accgactggc tgcaggccca
    11461 ctttcccgat cgggcggccc acgtgctcag cctgctgcgc cagctccgcg gcggcaaact
    11521 caacgacccc cgcttcggcc accgcatgcg cggccagggt gtgctggccg acctgtacag
    11581 tcagcgcctc gcccgcgccc ggcagcgcct gggactggat cgcccccggc gaaccctcaa
    11641 caccggcgcc ttccgtccgc cggcgccgga cggcgcccag ctgcgcctgt tctgaacccg
    11701 gccgtcagcg gcggtcgcgc ctcaacgcca cagatgcacg cccccgcccc agccgaaatg
    11761 cccacccgga tagccgtgac gatacgggta gccatagggc cacagggggt cgtaatagta
    11821 cggcgcagca cgacgccggt cccgctccgc ctcggcccgc gcacgatcct cggccgccgc
    11881 tgccgcggtg tcgcggcgga cgcgttcggc ggcggcctgg tcctgggccc agcgggcctg
    11941 ggcctcgttg agcacatcca ccacctccgg cgcgacaccc tgggcctgca aacgcgccgc
    12001 ctcgggcgca gggatggcat ggcgggttcc cgtcgcgcgg atgcgcgcca cgagatccgc
    12061 cgacggggtt cccgcacggg agagggccac gatctcgtcg agcgcgagcg ggcgaagcgc
    12121 cccacgcgcc gcatcgatgc gcgccgcatc cgcagggtcc aggcgcaaca gcgggggcgg
    12181 aggggacggc tgcgtggcac agccggtcac cagaacgaac gccatcgcca ccacgcgcca
    12241 ccggttcatc gcaccgcctc cttcagcgtg ccgccagctt ttcgtacaga ccgaccacca
    12301 ggtccatctg ctccaggatt cgttcgctgg tggtcgccac atcggccgca gcccgcttgg
    12361 gatcgacacc ctccttcagg accagggcgt tctggaagaa gaaccactgc tgatccacca
    12421 ggcccagctc ggtattgatc tggggagaat tggtggctgc gccccggagt tccgcctggg
    12481 ccttggcaaa ttccttggtg gcctgatcca gcatctctgc cgcggggccg gcgttcacat
    12541 tcaactgacg gaaaaaatag gccttggcca tgcgctggga cagcatgcgc tgacgcccgg
    12601 cgagcgccac caggtgtccg gacgggctgc cgaaggcctt ctcgtaggcc acggtgagct
    12661 tgtgcgccac ccccagcacg gcctcgttgc catcccacac cgccttcggg tcgctgcgga
    12721 tgtcacccag cagggggcgg tagcgggacc actcgctttc cagctgggcc agcagggtgc
    12781 gcacctcgtc actgggcagt tgggtcttca ggtcggccag ttgcaggtcg aaacgggtga
    12841 ccgtctggtc gaggatgctc cgcgcccggt ccggcagcac gcccagcacg ctcatcagcc
    12901 aggccttggc ggatcgctgc gagagcatac gctgccgccc cgcgcggttg atggcccccg
    12961 gcagggcaat gcccgcaggg gccggcgccg cgcccagcgg ggccgctgcg gccatcgctg
    13021 ctgcggccat caggaattcg cgccgctcca tgctagcctc ccgtgccgcc cacggtcagg
    13081 ccgtcgatgc gcagggtcgg ctggccgaca cccaccggca cgctctgacc gtccttgccg
    13141 caggtgccga cgcccggatc gagggccagg tcgttgccga tcatggacac ccgggtcagg
    13201 cagtccggtc cgttgccgat cagcgtggcg cccttgaccg gccgggtcac cttgccgtcc
    13261 tcgatgaggt agcactcgga cgtggagaag acgaacttgc cggaggtgat gtctacctga
    13321 ccaccaccga agttgaccgc gtacaggccg ttcttgaccg acgcgatgat ctccgccggt
    13381 tcgtgctggc ccgccagcat gatggtgttg gtcatgcgtg gcaggggcag gtgggcaaag
    13441 gactcgcggc ggccgttgcc ggtgggggcg acgcccatca ggcgggcatt gagcatgtcc
    13501 tgcatgtagc cggtgaggat gccgtcctcg atgagcacgg tgcgctgggt ggggttgcct
    13561 tcgtcgtcga tggtgaggga cccgcgccgg tccgggaggg tgccgtcgtc caccaccgta
    13621 acgcccctgg ccgccacctg ctgaccgatg cggccggaga aggcggagct ttccttgcgg
    13681 ttgaagtccc cttccagccc gtggccgatg gcttcatgga gcaggatgcc gggccagccg
    13741 ttgcccagca cgaccgtcat ggaccctgcc ggggccggac cggcgcccag gttgacgcgg
    13801 gcctgatgca ccgctgcgcg ggcgtagccg gccagcacct cgtcgctgaa gtaggcgtag
    13861 tcgtagcgcc cgccgccacc gccggagccc tgctcgcggc ggccattctc ctccatgatg
    13921 accgacaccg acacccgcac caggggccgc acgtcagccg ccagatggcc gtcgctgcgc
    13981 gctaccagca ccacttccca ggagcccacc aggctcgcca tcacctcgcg cacgcgggga
    14041 tcctcggcgc gggccatggc ctcgacccgc tccagcagac gcaccttggc cacgtcgtcg
    14101 agggagccgt ggggattgtc gccccggtac agggcgaggg gcggcttctt caccgcctcc
    14161 accttgtggg tcttgcgccg gccgctggcg gcgatggcgc gggtggcctc ggcggcggcc
    14221 ttgagggcct tcaggctgat gtcgtcggag taggcgaagg cggtcttctc accggtgacg
    14281 gcgcgcacgc cgacgccctg ctcgatgttg aagctgcccg acttgacgat gccctcctcc
    14341 agactccagg cctcggagcg gtggtactgg aagtacagat cggcgtaatc gagcttgtgg
    14401 cggaagagct tgccgaagac cttgtcgaga tcctcgaagt ccaggtcata gggcttgagg
    14461 aggagcttgg aagcgatctt gagggggctg gggggcggat tttttgtcat ggtcggggtg
    14521 cagctcaaag aatgcgatgc tggagggcgg gcaggctggt acggacctcg gcgatgcgcg
    14581 ccgggtccag gtcggccacc accacgccgg ggccttcggc gcgacgggcg agcacttcgc
    14641 cccaggggtc gatgatcagg ctgtcgccat aggtgcggcg gccgctggga tgcacgccgc
    14701 cctgggcgct ggccaggaca tagcactggt tttcgatggc gcgggcgcgc agcagggtct
    14761 cccagtgggc gcggcccgtg gtgtaggtga aggcggcggg caggacgatc aggtccactt
    14821 cgcccatcgc ccggaagagc tccggaaaac gcaggtcgta gcacacggac aggcccacct
    14881 tgccgcaggg cgaatcaaag gtgaccacct cccggccggg ctcgatggtg gccgcctcgt
    14941 catagcgctc ggcgcccttg ctgaagccga agaggtggat cttgtcgtag cgggcgaccc
    15001 gctggccggt gtcgtcaaag accaggatgg cgttcttcac ccggcccggg tcgctgccgg
    15061 cgaggggaat cgtgcctccg atcagccaca cgccgtgacg ccgcgccgca tcctgcagga
    15121 aggcctggag cggaccctcc ccgtccggtt cgcacaggcg cagcttggcg gtctcgtcgg
    15181 cggtgatcag cgggaagtat tcgggcaaca ccaccagccc cgcaccagcc gcggcggctt
    15241 cgccgatgag ccgctcggca gccgccagat tggcggccac gtcggggccg gacaccatct
    15301 ggacggcggc gatgcgatgg acagaactca ttgaccgatc ctttcggcgg ggttggccgg
    15361 cgcggcaggc agacggacgg cggcgatctt ctccaccttg gggtcggacc agctgccggt
    15421 caccgcgtat tcaaagctga acagtttttc cacggggtcg cgcagggcct tctgcaccag
    15481 ataggcggcc agccccacgg ccggatggat caccccggtg gtggccaggg ccgagcccac
    15541 cgccaccgat tcggagaggg tcggctgcac cttgacccgc aggctctggg tttcgcgcac
    15601 cgcgtccgcg tcgccgctca tgaatacgcg cgccgccgga ccgacaagct ccagatcctc
    15661 ggtgcgcagc accccgtcgt tcatctggat gctaccggaa atgcggtcga aggcgaatcc
    15721 ttgggagaag acgtcgcgga aatccagggt gatgcgccgg ggcagagcct gcaggctgag
    15781 caccccgagc aggcgcccga cacccggttc gagctggctg aactggccgc cttcgctgcg
    15841 cacgttcatg tggccgttga ggctcgccag atgcagcgcc gtgggcggcc cgcgccagcc
    15901 gatctcgccg tccagctgcg ccttgccctg gcgcaccgcc tcgggatagc ccaggcgggc
    15961 cagcatcttc tcgatgctgc ccacgtccag cttgaagttg aggcgggtct cctcctttgc
    16021 gccgggacgc caggagccat cgccgctcag ggtgccgtcg gggctggcca gactcaggtg
    16081 ttcgaggcgc caggtatcgc cccggttcgc ggccttcagg tccagcttgc cgagggcccg
    16141 cccgcgcagc acgaagctgt cggccgtcac gtccaggccg ggcagttcgg acaggcggtc
    16201 agcgctgcgg cccgcctcgc tgccgctgcc cgaggtaccg ccgctgatgg ccagctggcg
    16261 gaaacgcgcc tggaggcggc cgcgcccctg gtcgcgccac gtcaactcac ccgtcgcctc
    16321 gcggctcgcc agcctggctt gccagccgcc ctcctccatc accgcccgca gattcacgtc
    16381 gttcaggcgc tggccgaaca tgcgcagctc gccggccttc agggccacgc cggagatcgg
    16441 cagcccgccg ccggccccgc tgccgccatt gccggcgccg ccaccggaga gggcgcgacg
    16501 ccacgcgtcc acatcgagcc ggtcggccgt ggccgccagc aggacgccct tgtcggccat
    16561 gcgcaggggt tcgtcgagag ccatcccgcc gcgcaggggc gaccaccgtc caccctcccg
    16621 gcgccgggcc gtctgcatct gaaagctcgg accgatggcc agccggaagc tgtcgcccgc
    16681 cgtgctgccg cccgccaggt aatcggcgcg caggggcatc gccgcggcgg gcgatttgtt
    16741 gaagggttcc ggcaggctcg acgcgatgcc ctgcaggccg ctgtccacat gcacctcgag
    16801 cccgccctgg cgcggcaccg cgatcgtgcc cttccaggcc gtggcaccgg acaggtggtc
    16861 gaggagcggc tcgtccagct cccggcgcag ggccgcgatg ctggcgttgc cctggatcgc
    16921 cagcagcacc gtgccatccg ggcgggtgcc gccgctgacg ctggcaggct cgccatacag
    16981 cagggccgtg ccgctgcgca cgacaagctg gttctcggtg aagccgatgc gcccggcggc
    17041 gtccgtgagc accggcagca ccggatcggg ctggaccttg ttacgggcga actggaactc
    17101 gcccttcacc cgcgacgtct ccatccggtg caggggcagg gcgagatgca ggtccagcgt
    17161 gccgacgccc tcggtgcgga aaccgtcggt gaagtggttg atgcgtgcgc ccaccgggct
    17221 ttcggcgatg aaacgcagga aatcctgggt cggcccgctg gccttgccgt cgatggagag
    17281 cacctcgtgg gtctcgaaat cgggcagcac gacactcacg tccttcagct ccacgccgaa
    17341 gatgcgcccg tgctgggcct tgatgctcat ccccgcggcc tcgaagacca gctcgccggt
    17401 gacgtcctcg atcttcggcc agccaggagc gtagtcgagg ctgccgtcgc tgaccttggc
    17461 ggtgacgcgg aagacaccgt ccttcggatc gcggaacggg aacttcgcca gattgcccct
    17521 gagcaccagg cgcgcatccc tggcgcggcc accggtcagg ctgtgctgca gccagtcccg
    17581 cgcccgctgg ttcaccacgc ccggcatgta gcgccacacc gccgtgctct cggcctccgc
    17641 caggcgtgcc tgcaggtcga tctcccccgg cgtgtccggc cgcgcccggt agcgccccgt
    17701 cgccgtgccg cgggtgtccc ggttggcgaa gctcacgctg cccagattca cctccagcat
    17761 cccgtccggg tggctccagg tgccgtccgc ggccatttcc tccagggcca gacgcgattc
    17821 cgggaagacc tggggcagat ccagcgccgc ctgccggccg ctcaggctga agcgcccgcc
    17881 ccggtcgttg ccctcgatgc gtcccgacag ccccgcgaag ccgggccacg cgccgcccgg
    17941 ccggatgccc aggccggcga acttcgcatc caccgaatag gtggccagcg ccccggcctc
    18001 gccggtccat ttgaggttga ggggcgccac gctgccgctg ggcgccagat ccgccaggcg
    18061 cctgcggaag cccggctcca caggcaggcg ctcggccagt tcggccagca cccccagatc
    18121 gagctggttg gcgacaaact cgccgcgggc cggcgtcccc gcccgccccg gctgccagcg
    18181 caggaagaag tcggtgggcg ccaccttcag cccgtcgcga gtctccaggg ccaggcgctt
    18241 gccgctcgcc tccacccgcc cggcgtcgtc ggcgtagcgc aagcggccct ccgcatgcat
    18301 tgcgtcgatg gcctccagct ccggcgccag ccgcgcccgc gcgtcgcgca gggcaaaatc
    18361 ggcagtgatc cccttcaccc gcgcctcgcc gaactccagc caggcccgca cgcccccagc
    18421 ccccgccaga tccagcggat agtccaccca ggtcttccag gcgcccagat cggcgtaatc
    18481 gaggccgatg aagacctcac cgcgccagct ggccggctgc tgcggatcgc gtccgcgcag
    18541 atcgccacgc acgtccagag ttgcggcgag ggccgacggc ggcacggcct gcagcccgaa
    18601 gcggtgctgg ctgccgcgat tctcgaggcg cagattcacc tgccgcagct ccagcgccgg
    18661 cgcgccgcgg cgggcatcct cccaatccac gcgggcgtcg cgcaccacca cctcgcgctg
    18721 atccagcagc cagggcagga agtcgcccga ttcaccctcg gcattcacct gcaggcccgc
    18781 cacgaagagg cgcccgtccg cgtcccgacg caattcgaga tggggcgcca ccacctccag
    18841 ccggtgcaga cgcaatttga gcaggggcaa tgaggtccac cccagctcgg tgttcacctg
    18901 cgccagcgtc agggccggct cgccccgctc atccagcagg gtcagattgt ccaggcgcag
    18961 cgccggccgg aacccgtgcc actgggcgga cagggcgccg atcgccaccg gccgtccgag
    19021 ggaacgggac agcagcgccg ccacatcctc gcggtaagcc gggacgttgg gcagcaccgc
    19081 ccagcgcagc gcgagcagac cggccccggc gacgaagtac accgcgatca gggcccacag
    19141 caggcggcag gcccacacgc ggggcagatg cagcagggag aggaggcgct cacccgagaa
    19201 aggggtgagg aggcgccgga gtcgggtcat caggcttgac gaaaatcagt ccggcggaca
    19261 taagacgggg cgaacatgac aggactccat tctaccaacc gcccacccac catgtccgaa
    19321 cagcctgaca ccctgcaccc cctggtggcc gcggccaccg cccactcccg cttcctgcgc
    19381 cgccagctgg acagccgccc gtggctcgcc gcgcgcctcg ccgccagcgt ggacgcgccc
    19441 ctggacgcca ccgccctgag ggacttcctg cgtgccgagc atgtggacga cagcaccctc
    19501 aagcccgccc tgcggcgcct gcgcgcgtgg atcatcggcc acgtgatggt gcgcgatctg
    19561 gccggcctgg ccagcctcac ggaagtcacc gagaccatga ccctgctggc cgacattgcc
    19621 gtcgaagcca ccgtggatct gcaccgcgcc gccctggtgg cgcgccacgg gcagccggtc
    19681 tcggccgacg gccgcgagca ggaattcatc gtgctgggca tgggcaagct gggtgggcgg
    19741 gagctcaatg tctcctctga catcgacctg atcttcgtct atcccgagga cggcagcacc
    19801 gccggcccca agatcatcga caactacgag ttcttcacca aactcggccg cgccgtcatc
    19861 caggccctca acgatctcac cggcgacggc caggtcttcc gcgtggacat gcgcctgcgc
    19921 cccaacggcg actccggccc cctggtggcc agcttcgaca tgctggaaaa ctacttcatc
    19981 acccagggcc gcgaatggga gcgctacgcc tggatcaagg cccgcgtcat gaccggcatc
    20041 cgcggcgccg agctggccgc catcgtgcgt cccttcattt tccgcaagta cctggacttc
    20101 ggcgccatca acgccatgcg cgagctgcac gcccagatcc gccaggaagt cgcccgcaag
    20161 gacatggcca acaacgtcaa gctcggcccg ggcggcatcc gcgagatcga attcatcgcc
    20221 caggtcttcc agctcatccg cggcgggcgc gacaaagccc tgcagatcaa gcccaccctg
    20281 gccgtgctgg gcctgctcgt ggagcgcggc atcctgtccc gcgagaccgg ccgcgaactg
    20341 gccgccgcct acgacttcct gcgccgtgtg gagcatcgcc tgcaatatct ggacgacgcc
    20401 cagacccaca ccctgcccgg caacccggac gaccaggcgc gcctcgcccg cagcatgggc
    20461 tttacccact acgaagccct gctggaggag ctggacgacc accgggccca tgtgggccgc
    20521 cacttcaacg ccgtcttcgc cgaccccacc ggcaagggcc acgacctgga caccctgtgg
    20581 tcctccgccg gcgacgaagc cctcaccacg gccgaattca cccgcctcgg ctatcccgac
    20641 cccgccgccg ccgcgcgccg cctcgccacc ctgcgcgccg gcatccgcta ccagcagatg
    20701 ccccccagca tccgcagccg cttcgacccc ctgatccccc gcgtcgtcga agccgctgcc
    20761 cccacgcccg acgccaacgc caccttcgag cgcctcctcg acctccttga cgccatcagc
    20821 aagcgcggcg cctacctggc cctgctccag caatatcccc agaccctcga aaaggtcgcc
    20881 cagatcgtca gcgcctcagc gtgggccgcc cagttcctca accgccaccc catcctgctc
    20941 gacgaagcca tcgacccgcg catcctcgaa gaagagctcg acctgggtga attccacagc
    21001 gaactcgccc gccagctcga cgagatcgag cccgacatgg aacgccagat ggacctgatg
    21061 cgcgagcagc accacgccca ggtgttccgc ctgctcacca aggacatcgc cggccacctc
    21121 accgtcgaac gccacgccga cttcctctcc gccctggccg acatcatggt ggacctggcc
    21181 gtcaccatgt gctggcgcaa gatcaggaac cgccaccggg acgacccccg cttcgccgtc
    21241 ctcgcctacg gcaagctggg cggcaaggag ctcggctacg cctccgacct ggacatcgtc
    21301 ttcgtctacg acgacgacgc ccccgagtcc gccgagctct acacccgcct cagccagcgc
    21361 accaacacct ggctgtctgc ccagaccccc gccggcagcc tcttcgagac cgacctgcgc
    21421 ctgcgcccca acggcgattc cggcctgatg gtcgtgtccc tcgacgcctt ccgccagtac
    21481 cagctcgaga acgcctgggt gtgggaacac caggccctca cccgcgcccg cttctgcgcc
    21541 ggcgaccggg ccatcggccg caagttcgaa gccatccgcg tcgaagtcct gcgccagccc
    21601 cgcgatctgg ccaaactccg cgacgaagtc atcgccatgc gccagcgcat ggtggacgcc
    21661 caccccgtgc gcgccggcgt gttcgacctc aaacacagcc gcggcggcct catcgacgtg
    21721 gagttcatcg tccagtacct ggtcctcggc cacgcccaca cccaccccgg cctcaccggc
    21781 aacctgggca acatcgccct cctccccatg gccgccgaac tcggcctgat cccccccgac
    21841 gacgccgctg cggtggccga cacctaccgg gaataccgcc gcctgcagca cgccctgcgc
    21901 ctcaacaacc aggccccgcg ggtagacccg gcgctggtcg ccgaaggggc cgcggcggtg
    21961 cgggatctgt gggcgcaggt gctgggatga tgggcgcgtg cccgctcggg tgagggccgg
    22021 catgccgggg gcgccgtccg acgttcgcag ctgacgggca atgcgcggcg ctccgcacgg
    22081 aacgtgcccg ggctcagggc tgtcgtcggt cgattcaccg gaagggtgtc gggcgcagcc
    22141 cacctccgcg tcctcgcccc gcgcctgggt cataatccgc ccccatctcc ccttgcaggc
    22201 cgcgccccat gttcccctac acccactacc tcttcgacct ggacggcacc ctcatcgatt
    22261 cggcaccggc gatcctggcc agtttccgcg aggccttcgc gcgggccggc gtggcgccgg
    22321 tgcgggccat cgacgccagc gtgatcggcc cgcccctcgt cgagaccctg cagttgctcg
    22381 ccggccgcac ggacgccgcg ctgatcgaca ccctggccgg gcatttcaag gccagctacg
    22441 acaccacggg ctaccaggcc acggccgcct atgccggcgt cggcgagatg ctggcgcgcc
    22501 tcaagggcgc cggacgttac ctggccatcg ccaccaacaa gcgcctgcat cccaccgggc
    22561 tgatcctggc gcatctgggc tgggacgggt atttcgatgc ggtgtatgcc ctcgaccgct
    22621 tcacaccgcg cctgccggac aaggcggcga tgatcgcccg cctggtggcc gacgagggcg
    22681 tgccgcggga cggcgcggtg tacatcggcg accgcagcga ggacggggaa tcggccgacg
    22741 ccaacggtct gcccttcatc gccaccacct ggggctacgg cagcctgacg cccgccgacc
    22801 tgaagcccca ctggcgcgcg gtgaacaccc cggccgagct gggctgaggc accatgaccg
    22861 acactgccga tggcgcagcg gcgaccgcgg gcatcctcgc gccgccgccg ctgctctacc
    22921 tgggggcctt cttcgccggg agtctggcgg aggccctgtg gcccacggcc tcctgggcga
    22981 tgaccggatg gctgcgcggg gcgggcctcg tcctgttcat cgccagtgcc gccttcgccc
    23041 gctgggcctt cgtcacactg cggcgcatgg gcacctcggc aagtccgcgt caggcctcgg
    23101 cggctctcgg cacggacggg cccttcggct ggtcgcgcaa ccccatctac gtggccatga
    23161 ccggcctcta cctggggctc accgggctgc tcaccagccc ctggccgctg ctgccccttg
    23221 cgccgctgct ggtggcgatg cactggggcg tgatccgccg cgaggaacgt tatctgcacg
    23281 cccgcttcgg tgcggcctac gtggactacc ggcgccgggt acggcgctat ctgtgaaccg
    23341 gtcgggcggc gtcagagcgg cggcccgctg aaatcctcgg tggcggtgcc cgccatgatg
    23401 gccagcagcg cgtcgaaatc ctcgggccgg ccaatcatct tgaccagcgc gtagccgtcg
    23461 aagaccgaga aggacagctc gtcgttgagc agcgcctcgg cgtcggtggc cttgtcggtg
    23521 agcgtctgca ggcgggccag gatggggcgg agctggtcca gggccgccag atcccggcgg
    23581 aaagccagca gatcgaagtc gtccggcatg atgaggggct cgtgggcgaa gatcttgagc
    23641 atcctgcggc acagggcctc gccggtggag cccaattcgc ccatgccgta gcgctccttg
    23701 gagtcgagga tgtagtagcg catgaacagg cgttcgaggg tcgtcagcgc ggcctcgatc
    23761 tgttccattt cggcggcgct gaagcggacg ggcagctcgg aagaactcat gcgcttctcc
    23821 ttttccaggg ccgaacgacc atgtcggcca cggcgcacag tgtagtgcga gaacatcccg
    23881 gcggtatagc acctcgacac cgcccatcat gtcctgcgca tggaggcgcc cctgcccgac
    23941 gcgtacaatc cgagcatcgc cgcgccccct cgaaaagcct ctccaaaaag ccgtccatga
    24001 ccgcccccgc cgatccgctc gcggccgccc acgccgccta cacgcccctc agcctgcgcg
    24061 tctacgacgc ggtggtgatg ggcgtctcga acccgctgct gtggaagtgc ccgaccgccc
    24121 gcctgcgcgc ccacttcgac cggcacgtga gcggcaacca cctggacatc ggcgtaggca
    24181 ccggctactt cctcgaccat gcccgcttcc cggtgcacgc cccgcgcatc gtgctgatgg
    24241 acctgagcac ggcggccctc gcccacgctg cccgccgcct cgcccgctac cggcccgaga
    24301 ccgtcatcgg caacgtcctc gcgccactgc cggtgcccgg ccggcggttc gattcggcga
    24361 gcctcaatta cgtgctgcac tgcctgcccg gccccttcgc cgccaagctt tccgccttcg
    24421 accacatccg cccgctgctc aaccccggcg cggtgctctt cggcagcacc ctgctgcagg
    24481 gcgacgcccc gcgcaatgcg gcggcccgct cgctgatggc ctggtacaac cgcaagggca
    24541 tcttctccaa cgccggtgac gacttcgcca ccctgcaggc ggcgctgagg cagcgattcc
    24601 ggcaggtggc gcttgaacgc gtgggctgcg ccgccctgtt cgccgtccgc aactgacctc
    24661 ctccgagcac gtcgcaagtc ccccgcggcg cccgcgctgc acggcggacg gacgggccgc
    24721 ccgcattcaa gtctgtcgta accccggcca taaccggact ggaactcggc gccgcgcagg
    24781 atagctcgcc tgcacggccg cccgcccggg ggaccgcgca gcccgatccc cttcctgccg
    24841 aggagtcagc atgcgcatcc agtcgctcca cccgttcctg tcacgacccg tctcgcggca
    24901 tcgtgctccg ccagcccccc gccccaccct gcgcgccctc gccggcacgg ccctggtcgc
    24961 cctgacggcc ctgagcctgc cggcccaggc cagctgcttc tggtccacgg ccaagtccta
    25021 cagcggggcc gtcggcctgc tcgtgggcgc cagcggcctg atccgcacgc cgaccggtga
    25081 ggccgtgcgc gttggcgtga cggccggcac cgccgtgggc gagctcatcg attacggcat
    25141 ctcccattgg tgggatccca gcctggagct cgtcccggac gacccctcgc aggtcgtgac
    25201 cgatcccgcc cagagcctca tccagtcggc catcaccagc ctgctcagcg acttccgcgg
    25261 ctccgcgccc tacgccacca catgggcaga cctgcaggcc ctgaacgcaa ccgaagcggg
    25321 cttcggcgac gccatgacga cgatgctgag cagcggcgtc agcgcatttg tccacatcgc
    25381 ccagggcgaa gccgcctaca cggccttcgg acgcggctcc gcccaggtgc tggcggccgc
    25441 ggcgcgggtc aatgacgacc tccagcgctt cagcaccgcc cagtcggatc tggtgcccta
    25501 cctggcgccc ttcgatggca cgccgggatt tcccaccgtc acgaaggcca gctaccagag
    25561 cttcctcgac ggcatcctca gccggggggc agctggcctg ccgccgggcg aggtgagtgc
    25621 ggggcagcgc ctgctgagcc tcgccggcgt ccacttcgtg ccgggcaagg ccctcgacag
    25681 ttccctggtg acctacctgt ccgccggcga caccggcaac gagatggccc agatccccga
    25741 cgccggcatc ggcctcagcg gcctgatcgc cgcacgggcg cccggcttcg ccggcgactg
    25801 ctgcacctgc ggcgacttct gcctgccgga gcccgccagc gtggccctgg tcctcaccgg
    25861 cgtcaccgga ctcggcctgc agcgccgccg ccgcgccatc gacgatctgc cgtctgccgc
    25921 ctgatccacg gcgccccgcc gggcggcccc cgcgccgccc ggaaataccc ccctttcccc
    25981 cgccttatcc gccctaaggc cgtgccctgc cacgcataac atcggctcca tccctccctg
    26041 gagctgcgat gaaagccgtc attctcgcgg gtggcctggg cacccgcatc agcgaagaga
    26101 ccgtctcccg ccccaagccg atgatcaccg tcggcgggcg gccgatcctc tggcacatca
    26161 tgaaaatcta ttccagccac ggcatccacg acttcgtgat ctgctgcggc tacatgggtt
    26221 acgtgatcaa ggagtacttc gccaactact tcctgcacat gtcggacgtc accttcgaca
    26281 tgacccacaa ccgcatggaa atccacgagc gtcacgccga gccctggcgc gtcaccctgg
    26341 tggataccgg cgaatccacc cagaccggcg gccgcctggc ccgcgtgcgc cagtacgtgc
    26401 aggacgagga cgccttctgc ttcacctacg gcgacggcgt ggccgacatc gacatcggcg
    26461 ccgagatcgc cttccaccgg gaacacggca agctcgccac cgtcgccgcg gtgcaaccgc
    26521 cgggccgctt cggcgccctc aagatccgcg ccggtggctg cgtcagcgcc ttcgtcgaaa
    26581 agcccgctgg cgacggtggc ttcatcaacg gcggcttctt cgtgctgtcg cccaaggtgc
    26641 tggatgccat cgacggcgac cagaccacct tcgaaggccc ggtactggcc agcctcatcg
    26701 cccgcaagca gctcgccgcc ttcccgcaca ccggcttctg gcgcccgatg gacaccctgc
    26761 gcgacaagac cgtactggaa gaactgtgga ccagcggcaa cgccccatgg aaaacctggt
    26821 gaatcccgaa ttctggcgcg gccggcgggt cttcatcacc ggccacacgg gcttcaaggg
    26881 cagctggctg agtctgtggc tgcaggccct gggcgccgag gtgcatggct acgccctggc
    26941 accggacacc gagcccgccc tcttcaacgt ggcccacatc gcccgcggca tgcagtccac
    27001 gctgggcgac ctgcgcgacc tgcctgccct ctgcgccgcg ctgcacgccg ccaggccgga
    27061 gatcgtcctg caccttgccg cccagcctct ggtgccgcgc tcctacgccg agccggtcga
    27121 gaccttcgcc atcaacgccc tcggcaccgc ccacctgctg gaagccgtgc gcctgcagcc
    27181 gggcgtgcag gccgtcgtgg tggtgagcag cgacaagtgc tatgagaacc acgagagcct
    27241 gtggggccac cgggaagacg cgcccctggg cggccacgac ccttactccg ccagcaaagc
    27301 ctgcaccgag ctgatcgccg cggcctaccg gcgctccttc ctcacccccc gcggcgtggc
    27361 cctggccacc gcccgcgccg gcaatgtcat cggcggcggc gactggaccc ccacccggct
    27421 ggtgcccgac atcctcgccg ccttcgccgc cggcgccgcc gtcaccctgc gcaatccgga
    27481 agccgtgcgc ccctggcagc acgtgctgga acccctcgct ggctacctgc tgctggccga
    27541 gaagctggtc ctggacggcc tgccctgggc cgaaggctgg aacttcggcc cggacgacac
    27601 cgattcccgc agtgtggcgt ggatcgtcga gcgcctcgcc gccggctggg gcgatcacgc
    27661 ccgctggcgc gtcgccgacg gcccgcgcat ccacgagacc caggccctgc gcctggactg
    27721 caccaaggcc cgccgccgcc tgggctggca tccgcgctgg tccgccgaga ccgccgtgga
    27781 ccgcagcctc gcgtggtacc gggcgtggcg cactggcgcc gacatgcgcc gggcctgcct
    27841 cgacgaaatc accgcctttg gaacccgccc atgagcgtcc cccaaacccg cctcgccgtc
    27901 accgacccgc tggccgcagc caccgccctg cagcgccggc gcgtgcaggc cgacgccgcc
    27961 ggacgcatcg ccctgaacct gcccgagctg gccggccagg acctggaagt catcgtccgc
    28021 cccgcccgcg cacccgccga gccctccgcc tggcggcccg acctggaccc ggtgccgccc
    28081 tccggcaagg tcatcggcga cgccgagcgc aacgccatga tcgacgccgc cctcgacggc
    28141 tggctcacca ccggccgctt caacgacgcc ttcgaggcgc gcctcgccgc cttcgtcggc
    28201 agccgctacg ccctcaccgt caactccggc tcgtcggcca atctggtggc cttctcggcg
    28261 ctcacctcgc cacgcctggg cgaacgggcg atccgccccg gcgacgaggt catcaccgtc
    28321 gcggccggct tccccaccac cgtcaatccc atcctccaga acggcgccgt gccggtcttc
    28381 gtggatgccg ccctgcccgg ctacaacctc gacgtgacga tgctggaggc ggccctgtcg
    28441 ccgcgcacca aggccatcat gatcgcccac accctgggca accccttcga cctggacgcc
    28501 gtctgcgcct ttgccgaacg ccacggcctg tggctggtgg aagactgctg cgacgccctc
    28561 ggctccacgt ggaaaggccg ccgcgtcggc accttcggcc acctggcgac cctctccttt
    28621 taccccgccc accacatcac catgggcgaa ggcggcgcgg tgctcaccag cgacccgcaa
    28681 ctgcgccaga tcgccgaatc cttccgcgac tggggccgcg actgctggtg caagccgggc
    28741 tgcgacgcca cctgcggcaa gcgcttcggc tggcagctgg gcagcctgcc cacgggctac
    28801 gaccacaaat acacctacag ccacatcggc tacaacctca agatcaccga catgcaggcc
    28861 gcctgcggcc tcgcccagct ggatcggctc gacgctttca tcgccgcccg caagcgcaac
    28921 ttctcctacc tgaacgccgc cctggccgac gccgccgacg tcctgatact gcccgaagcc
    28981 acgccaggca gcgacccggc ctggttcggc tttcccctca ccctgcgccc ggaggccggc
    29041 gtccaccgcg tcgacctgct ccagtacctg gaccagcaca ggatcggcac gcgcctgctc
    29101 ttcgccggca acctgatccg ccagccctcc atggccgggc ggaccttccg cgtcgtcggc
    29161 gacctgccgg tggccgaccg catcctccac gacaccttct gggtcggcgt atggccgggc
    29221 ctcgacaccg cccagctcga ccacatcgcc cgccacatcc gctgcttcct cggagtcgat
    29281 ctatgaacaa gcccctgatg gtcttcgaaa tggccaacaa ccacatgggc gacctgcccc
    29341 atggcctcgc caccatccgc gccctgcgtg aggcctgcac cggcttcgag gacgtcttcg
    29401 acttcgcctt caagctccag taccgggacc tggacacctt catccacgcc tccgcccgcg
    29461 gccgcgacga catcaagtac gtcaagcgct tcgaggagac gcgcctgtcc gacgaggcct
    29521 tccgcgccct cgtcgccgag atggacgcct gcggcttccg caccgtgtgc acgcccttcg
    29581 acgagccctc ggtggaccgc atcgaggccc acggcatcca caccctcaag atcgccagct
    29641 gctccttcac agactggccc ctgctggagc gcatggccac cagcgccaag ccggtcatcg
    29701 cctccaccgc cggcgccact ctggaagaaa tcgacaacgt caccagcttc ttcctgcacc
    29761 ggggcaagga cttcaccctg atgcactgtg tcgccgaata ccccacgccc gacgccagcc
    29821 tgcaggtgaa ccagatcgag ctgctgcgca gccgctaccc cggcgtgcgc atcggctact
    29881 ccacccacga ggcgccggac aacaccctgg cggtcggcct cgccctcgcc aagggcgcca
    29941 gcgtcttcga aaagcacgtg gtcctgccca ccgagcgcta cgccgccaac gcctattccg
    30001 ccaacccggc gcagatccgc gcgtggctca gcgaggcccg ccgcgccctg gccatctgtg
    30061 gcacctccgg cgaccgctac gtcccctccg ccgccgagcg ggccagcctg cagtccctgc
    30121 gccggggcgt gttcgccacc tgcgccatcc cggccggcac cgagctcgac gccacgatgg
    30181 tggaattcgc cttcccgccg gtggacggcc agctcaccgc caacgactgg tccaagtacg
    30241 cccgcttcgt caccaccgcc cccatcgccg ccggccagcc cgtgctgcag gcccaggtgg
    30301 ccgaacacca gctgcgcggc aaggtgctgg agatcgtcac cgccgtgaag gccctgctca
    30361 aggccggcaa cgtggtggtg ccgggccagt ccgacctgga gatctcccac cattacgggc
    30421 tggagctctt ccacgaattc ggcctgacca tggtcaccgt ggtgaaccgg gaatactgca
    30481 agaagctgct ggtcatgctg cccggccagc tccacccgga gcagtaccac ctcaagaagg
    30541 aggaaacctt cgtggtcctg cacggcgccc tgatcctgtg gctggacggg gaagaacgcc
    30601 acgcccgggc cggcgacgtg atcaccgtcg cgcgcggcgt acgccacacc ttccgcaccg
    30661 aggagggcgc cgtcttcgag gagatctcct ccacccacga caggaacgac tcctactata
    30721 ccgacccggc cattgcgcag aacacccacc gcaagacctg gctcacctac tggatgtaag
    30781 cgccatgccg accctccgcg tcgccgactg gatcatgggc cgcctggccg acctcggcgt
    30841 ggatcacgtc ttcctgctgc ccggcggcgg agccatgtat ctggatgacg gcctcgcctg
    30901 cgagccccgt ctcacggccg tgccctgcca ccacgaacag gccgccgcca tcgccgccga
    30961 agcctgcggc cgcaccggcg cggcgggcaa tgcgggcttc ggcgtgtgcg tcgtcaccac
    31021 cggccctggt gccaccaacg ccatcactcc agtggccggc gcgtggatcg actcggtccc
    31081 cctgctggtg atctccggcc aggccaagcg cccggaccgc ctcaacggcc gccccctgcg
    31141 ccagggcggc gtgcaggagg tggatgtggt gcccatggtc cgcagcatca ccaagtacgc
    31201 cgtcaccgtg gaagcccccc aggccatccg cgcccacctg gaggaggccc tgttccgcat
    31261 gcaggacggg cgccccggcc cggtgtggct ggacatcccc ctcgacgtgc aggccgcgcc
    31321 ggtggatccg gacaccctgc caggctggac gccgcccgcg gccgaactcc cgccggcgcc
    31381 ggatttcgcc accctcgccg acctcctcgc ccgcgccgaa cgccccctcc tgctggccgg
    31441 ccacggcgtg cgtctggccg gcgcggcgga ggccttccgg cagttcaccg agcgcctgca
    31501 gatccccgcc gtgctcacct ggaatgccct cgatctgctg ccctacgacc accccgccaa
    31561 catcgggcgg cccggcgtgg tggccgcccg cgccgccaac ttcgcggtgc agaactgcga
    31621 cctgctggtg agcgtcggct gccgcctgga caacatcgtc accgcctaca acccggccgg
    31681 cttcgcccgc agcgccgtca aggtggtggt ggacgtggat ccccacgaac tcgccaagct
    31741 gcccatgccg gtcgagcatc ccctcgccgt ggatgcacgg gacttcctcg acggcctcct
    31801 cgccgccagc gccgacttgc cgcccctccc ctgcgacgcc tggcggacgc gctgcgcgtc
    31861 gtggaaagcc cgctacaccc agaacgaggg acgcgtcttc ccgcccgccg gccccatcgg
    31921 ccacgcccac ttcgtcgaag ccctgtccga cgccgccccg cccgacaccc tcatcgccac
    31981 cggcagctcc ggtctggcta tcgaattttt ctacgccggc ttccgcaaca aggccggcca
    32041 gcgcagcttc ctcacctccg gcctgggcgc catgggctac ggcctgcccg ccgccatcgg
    32101 cgcctgcctg ggcaacggtc ggcggcccat gctcgcggtg gaatccgacg gcagcctgca
    32161 gctcaacgtg caggagctcg ccaccctcgc cgggcagtgc ctgcccgtct gcctgttcat
    32221 cctcaacaac ggcggctacg cctccatccg caacacccag cgcaactact tccagggccg
    32281 ctacctgggc accgggccgg aaggcggcct gctcatgccg gatctggaaa agctcgccgc
    32341 cgtctacgac ctgccctatc tgcgcatcga cgactgcgcc ggtctcgccg acgccctcgg
    32401 ccgcgcccag gccctgccgc gcccctgcct gatcgacgtg cacctggtgc ccgacgaaag
    32461 cctcgccccc aagtgcgcgg cgatcccccg cgccgacggc tccatcgtct ccatgcccct
    32521 ggaggacatg agccccctgc tgcccctgga cgtcctgaag gcggagatga tcgtgcccct
    32581 gctgcccgcc tccctcgacg caccccgccc ggcctgagcc catgtccacc acctgccgcc
    32641 tgtgcggcca tcccctcttc ccggaaccgc tgctggtcca gcacgccatg cccgccgccg
    32701 cccagggtct tccgaccgcg gagcagctcg ccgacgacca gggtgtcacc cttgccctgc
    32761 accagtgcgc cggctgcgcc accgtgcagc tcgccaaccc gccggtgccc tactggcgcg
    32821 aggtgatccg cgccgccggc ctgtccgccg agatgcgcgc cttccgccgg gaacagttcg
    32881 ggcgctggat cgccgagcac gacctgaccg gccgcaaggt gctggaggtg ggctgcggac
    32941 gcggcgaata cctggccctg ctggctgaag ccggcgccga cgcccacggt ctggagtacg
    33001 ccccggccgc cgtcgccgcc tgccacgccg ccggcctgca cgcgcaccgg ggctttctcg
    33061 aagatccctc gatccgcctc gccagcggcc ccttcgacgg cttcgcgatc ctcaacttcc
    33121 tcgaacacat ccccgccgcc ccgctcacgc tgcaggccat cgccgccaac ctgacggacg
    33181 gcgccagcgg cctcgtggag gtccccaact tcgacatgat cctgcgcgca gggctgttcg
    33241 ccgagttcat tccggaccac ctgttctact tcacccggca gaccctcacc gccctgctgg
    33301 aacgcaacgg ctttgacgtg ctggactgcc gcccggcgtg gcacgactac gtgctctccg
    33361 ccaccgtgcg caagcgccgt cccctcgacc tgagcccgct cctggcctgc caggacgccc
    33421 tcaaggccgc cttcgacgcc ttcctcgccc gctttgcgcc gggccgcgtg gcgatctggg
    33481 gcgccggtca ccaggccctg gccctcatca gcctgatggc gctcgccccg cgcgtcgcct
    33541 acgtggtgga ttccgcaccc ttcaagcagg gccgctacac cccggccacc caccggccca
    33601 tcgtcgcgcc cgagcgcctc gccgacggcg aggtggacgc ggtgatcgtg ctcgccgcga
    33661 gctactccga cgaggtcgca cgcatcctgc gcgaacgcca cggcagccgc ttcaccgtcg
    33721 ccgtgttgcg cgacaaccgc ctcgacacgg tcattgcccc atgaccacgc ccagccccga
    33781 tgccctccgc gaggccatcg ccaccctgcg cgccgcgctg ggcccggcgc cccgtaacgg
    33841 cctgccggaa gacctgttcc tcttcgtcag cagcctcaca ccgctggtca acgtggatct
    33901 gctgatccgc gacgacgccg ggcggatgct cctcggctgg cgtcacgacg ccttctacgg
    33961 ccctggctgg cacgtgcccg gcggcatcat ccgcttcagg gaagacgccg ccacccgcat
    34021 cgcagccgtc gccgcccgcg agctgggcgc ccgggtgcgc ttcgagcccg agcccctgtg
    34081 catccacgag atcgtgaacg acgtgcgcga ggagcgcggg catttcatct ccatgctcta
    34141 cgcctgcacc ctcgacacgc cgccggacga gcgcctgcgc ttcgatccgt ccgcccccgt
    34201 ggacggcgcc tgggcatggc acgccgcatg ccccgacccg ctgatcgacg cccacgacct
    34261 ctaccgcccc tggcttggcc cccgcggcac gccgcgcccc acccgccgca gcggccgcca
    34321 gccccagcgc cactggcacc tcctctcggg acgccccgca tgatcaacag cctctacaag
    34381 aaccgcacgg cgatctattt cggcaccggc atggaagacg ccggactcac cgaactcgcc
    34441 cgcctcggcg cccgacacgt gctgctccac tacggccagg gcagcgtcac gcgcagcggc
    34501 ctgctggaca gggtgatggc cggcctcacc gcccggcaga tcgccgtcac cgaactgggc
    34561 ggcgtacagc ccaacccgac cctgccacgg gtgcgcgaag gcgttgccct gtgccgcgca
    34621 cggggcatcg acaccctcct cgcggtgggc ggcggcagcg tgatcgactc ggccaaggcc
    34681 atcgccgccg gcgcggcctt tgacggcgac gtgctcgacc tcatcgaagg ccgcgtgcgc
    34741 cccgccgccg cgctgccgct gggagtcatc ctcaccgtgc ccggcgccgg cagcgaatcc
    34801 agccccgcgg cagtcatctc cgaccccgcg cgcagcctca agctcgattt cgccgccgag
    34861 tgcctggtgc cggcgctggc cgtgctcaac cccgagaaca cccgcggcgt gggcctgttc
    34921 cagagccgct gcggcatcct cgacgccatc acccacgtct gcgagcgcta cttcacccag
    34981 accgactacg tggaggtgag cgaccgtctg tgcgagggcc tgatccagtc cctcatccat
    35041 tacggtcgcg ccgtggacgg ccgcctcgac gactacggcg tgcgcgccga gatcatgtgg
    35101 gcctgcaagc tcgcccatga cgacgtggcc ggcgtggggc gcaagcagga ctggtcatcc
    35161 cacgtgatcg ccaacgccct gggcggccac gtggaccagc cccacggcgc cctcacggtg
    35221 atcgtcatgc tgggctggat gaagcgcgcg gccgaaacca gccccgcccg cttcgtgcag
    35281 ttcggccagc gcatcttcgg cctcgcctca cccacggcgg ccgaggcgat cgccgccttc
    35341 gagcattaca tcgcgggcat cggcatgccg gtgcgcctgt ctggactggg cgtcgacctg
    35401 gccccggcca ccctggacgc ggtggtggat gtctgcgccc gcaagaaccc gagcggcacg
    35461 atcggcaact tcgtgcggct cggccccaac gacgtgcgga cgatcctcga aaccgcgcgc
    35521 tgagagagga gtcaccatga gctacaagat ccacatggac ggttacgcag agccgcgcac
    35581 ggtcttccag atcgaggcgc tgcacgcgtg gaagcacaag cagtacttcg ccccggtgat
    35641 cgtcgaggtc agcccggtgg gctactgcaa ccagaagtgc aagttctgct attccgacaa
    35701 gatcgagacc aagggcgaca agatccccga cgcgatcctg acccggctgt tctgcgacct
    35761 cgccggcatg ggcgtgcagg ccgtctccct gcagggcacg ggcgaaccgc tgatgcatgg
    35821 cggcctgccc gacgccatcg tggccggcgc cgggaagggc ctgcgctact cgctcaccac
    35881 caccggtgtg gtcttcaccg aaaagatcca gtccaggatc ctgcagcacc tcaccttcat
    35941 gaagtgcagc gtcctggaga acgaccgtga ccgctacgcc ctgatgcacg gctgctcgcc
    36001 caagcagtgg gaccagctca tcgccaacct cggcgccgcc gccaaccggc gcagccgcga
    36061 agggctcgac gtggcgctga tgggcacggt gtacgtcgaa ctcaacaact tcgaccgcct
    36121 gccccagatc atccgcttct accgcgacct gggcctcgac tacatcgtgg tgcaggaagc
    36181 cacctacacc gaattcaccc cgtcgaacca gtcgcgcaac acctccgaac acttcaccgc
    36241 cgccgagatc gacgccatga aggccgaggt gctgaagctc aacaccgagg acttcacggt
    36301 caaggtgcgt tttccggtgg atgacaccag ctaccacgtg ggcatgtaca aggactgctg
    36361 gaaggacgac cactgccagg gcatcaaatt cttcccgctg gtggccgccg atggcaatgt
    36421 ctattcgtgc taccgcgcct ggggcaaatc cgagttcagc atgggcagcc tgcacacgca
    36481 gagcttcgag gagatctggc gcggcgaacg ccgccaggag gtcgagcgcc agatcatggg
    36541 caccccgccg ggcggtgacg aatgcgccgt gtgcggcatc tccaagctca acgacatcct
    36601 ctcgaagtac gagcaaccca acaagtggcg gagcttcctg atatgagctg cgatctgtgc
    36661 atcaagcacc tgcccggcga gaccgactgc gtgtggcacc gggaacacaa ggaacacggc
    36721 aaggactacg gcccccacaa catcctgccg agcaacgtct gcccgatcat gtaccacacg
    36781 ctgtacccgt atttcctcgg ggcactgttc ggcgcccggt acgcgtacaa cgagcagggc
    36841 gactgccagg tatgctgccc ggcggccaag ggcgtggacg tgctggtcaa ggtgcgcccc
    36901 aacgacggca agttcgaggc cggcgtcgat gccgactggc gcgacgtgat ccacgccgag
    36961 gtcgtcgcgg tgcatggcga ctgcccctac cagcacaagg tgggtgaccg catcgtcttc
    37021 ccgaccaccc gcagcatgcg cacccagtac atgtgccccg ccggcttcaa caacctgttc
    37081 cccttcctga agatcgacat cccctcgtgc atcgacttcg accgggtgcg ctgccccgac
    37141 tggatggaga acatctacta ccgcatcgac gagaacagcc gcgtcaggaa ggactgagca
    37201 tggcccgcct ggcgctgatc tccctgcacg acaccaccgg cctggtggat gacgcccgtc
    37261 ggctgatcgg cgccggcttc accctgctcg cctcccgcga gtcctccagc ctgatcgccg
    37321 ccgccggcct gccggtgatc gacctggccg actacacggc gctcaccgag gacttcggct
    37381 ttccgccgac cctgcatccg aagctcgaac gcgccctcac cgcccgcccc gccgacctgg
    37441 ccgaacccgc catcgagctc gcctacatca acccctaccc ggccgacgtc ggcaatgacg
    37501 tgggcggacg cacgatcctg atgctcgccg tcaagggcca gcgcctcgcc gcctccagcc
    37561 gggccgacct gcaccgggtg ctcgcctgcc tcgaaaccgg cggggatctg cccgccctgc
    37621 ggcgccagct cgccgacgcc gcctgcttca gcgtcgcccg ccacttcgcc ggcctgatct
    37681 cgcccgacgc ccccttcgcc accagcttcg gcgaagcctg gctcgacctc gccgaaggcg
    37741 aaaaccccta ccagcgcccc gccgtagcct accgcagcag cgatccggac ccgctcgccc
    37801 tgagccgctt ccgcctcctc ggcagccagc cgccgtgctt caccaacgcc gccgacgccg
    37861 acgccatcct ccaccatctg tgcctgctgg ccgaaggctt tgagaagaac acgggcaccg
    37921 ttccctggct gtgcatcgcc gccaagcacg gtaacgcctg cggcatcggc gtcagccgcc
    37981 acgcgccgcg ggaagccatc gaccgcgccc tggccggcaa cccccgcgcc atctggggtg
    38041 gcgaggtgat cgtcaatttc cccatcgacg gcgagctcgc cgccgcactc ttcgccagcg
    38101 acgaacgcga agtccgcctc ggcagccgcc actggatgct cgacgtggtg atggcgcccg
    38161 ccttcaccgc cgaggccgcc gaactgctgg gcaggcgcga attccgcaag ctcctggcca
    38221 accccgcgct ggccgcagcg cgcctgcccc acggccgccg catgctccgc ccggtgcgcg
    38281 gcggcgccct cggccagccg gtggccgact acgtgctcga actggccgcc tgcgccggca
    38341 acgcccgctt cgacgaagcc gccgtcaccg acctcctgat cgcctggtcc gccgcctaca
    38401 gcagcaatca cggcggcaac gaggtggcgc ttgccagggg cggcatgctc ctcgccgccg
    38461 gtggcggacc gtctaccgtc gacgccgccc gcagcgccgt cacccgctgc gccgaacagg
    38521 gccatgacgc caccggcgcc gccttcgccg ccgacgcctt cttccccttc accgacgccc
    38581 cgcagatcgt cgccgacgct ggcgcccgct gtggcgccgt gcccgacggc agccgcaacg
    38641 acgacaccgt caaagccttc ttccgcgagc gccacatcgc cgtcgcctgg attcccgccg
    38701 ccttccgcgg cttctgccgc cactagaccc gcgccatgaa tgcccccgac tccccgctgc
    38761 gccgcctgct ccacacaccg cccgccaccc ctgcggcgcc ctgccgcgtc gccgacgtgc
    38821 tcgccggccg gccggtcgtc gtctatggct gcggcgacgg gctcatcacc ttctcggtgt
    38881 tcgtgctgga taaattcggc atccggcctg aggtctgcct tgacgccaaa ttcaccgccc
    38941 ccaccaccct cggcgacatc ccggcgatgg ccccgaccga ctaccggccc gatccggccc
    39001 tgctcgaccg cgcagtggcc gtcgtcaccg tcggcaagac gcggttccac gcggacatct
    39061 tcgccaccct gcgcggcctc ggcttcacgc gcatcgtgct ggcctcggac ctctacgaat
    39121 accacctctc ccacgccccg gccggcttcg aacagctcgg ccaaacctgg ttcgccgaac
    39181 gcgccgacgt catcgagcgc gcctacgccc tgcttgccga cgcaaaaagc cgcagcgtct
    39241 tcgaagccgt gctgcagacc cacgtcgcgc agatgcccgt acccattccc cgcgaccccc
    39301 tcgagcacca gtacttcccc cacgacgtac ccctcgccgc cggcgtcgcc cgcaccctca
    39361 actgcggcgc ctacgacggc gacaccgtgc gccagcttca cgcccgtcac ggccgcatcg
    39421 aggcccttgc ctgctttgag cccgacctgc ccaacttcac ccgcctgcgc gccaccctca
    39481 ccgagcatgc cggccagctc gccggcaccg tgatggcctt cccctgcggc gtgttcgacg
    39541 acgacgtgca gctgcgtttc gccgggggca atcgcatcaa cagcaccctc agcgacgacg
    39601 gcgacaccgt cgtccaatgc gtcgccctcg accacgtgct gccggacttc cgcccgacct
    39661 tcatcaacat ggacatcgag ggggccgaac cccacgccct gcgcggagcc cgacagctca
    39721 tccgggccaa ccgacccgac ctcgccatct gcgtctatca ccatccggcc cacctctggg
    39781 agatcgccct gcagatacac gcgctggtgc cggattaccg cttctacctg cgcaactaca
    39841 ccggcttccc cgccgagacc gtgctctacg ccagccgctg acccgccatg accgcctcgc
    39901 agcgcttcac cgccgagatc gaccgcgccg tcgtccgctt tggcgacggc tggacggccc
    39961 tgcgcggcgc gcgcctgttc atcaccggtg gcaccggcta cttcggacgc tggctgctgc
    40021 ccgccctcat ccgcgcggac gccgtacatg ggctcggcct cacggcgacc gtcctcagcc
    40081 gcgatcccgc gcgcttcctg gccgacgcgc cggaagtcgc tgccagcccg gcggtccgct
    40141 tccatcccgg cgatgtgcgc gactttgcct tcccggacgg cgacttcacc cacgcgatcc
    40201 atggcgccgc cacctccgcc gcggccacct tccatcgccg cgaggacgcc ctcaccaagt
    40261 tcgacaccac cctgcaaggc gcccgccggc tgctggattt cgccaccgaa cggcgcatcc
    40321 cgcgcctgct catgctcggc tccggcgcat tctatggccc gttgccggcc gaccgcgcgg
    40381 cgtacgcgga agacctgccc gtcgccccct cccccgacaa cctcgacgtg gccatcggcc
    40441 acgccaagcg cgccgccgaa ttcctcagcg ccgcctacgc cgaacgccac ggcctgtcca
    40501 tcagcaccgc ccgctgcttc agcttcgtcg gcccacacct gcccctcgac ctgcactacg
    40561 ccatcggcaa cttcatccgc gacgccctgc gcggcgaagc gatcaccgtc ggcggcgacg
    40621 gcacaccggt acgcgcctat ctctacgccg gcgacctggt ggtgtggctg ctcaccctgc
    40681 tggtgcgcgg cgcacccggg cgcgcctaca acgtgggctc cgaccaggcc gtcaccatcg
    40741 ccgatctggc acagcgggtc gcccgtgtgg tggcgcctgc ggtgccggtg cgcgtcctcg
    40801 gcacgacgac cgacgtagcc acccgcaacg tctacgtgcc ctccatcgag cgtgcccgtg
    40861 gcgagctggg cctcgacgta tggacgcccc tcgacgaggc catccgcctc accgccgacg
    40921 tggcccgcgc gggcgccgcc ccgacccaag cacggaatta ggcccgcctc tcccccctat
    40981 tccggccatc cggccaccgc cagcacccgc acaatgggtg cgacacctga aatggcaatg
    41041 tgcccttcag aaagccctga agccgccgta agtgccgaca ccatcgcgct ttttcaggaa
    41101 ccggccatcc cccggaagtg cctcgaaaag gcccgagctc cccgacagcc tgtcgccccc
    41161 cggcccaccg acggaccgga cccgagaaag agacggacgc catgaccact gcgctgattc
    41221 ccgattccgg cctgaccgcc gccgaggcgg ccaaacgcaa cgacctcgcc acccgcaaac
    41281 cctacgtggc cgccaagctc gcccgcattg ccgagatgga ggaacgcggc gagatcagcc
    41341 ccatcatccg gctcgagaag agctacctgt gcaacttcca gtgcacccac tgctcggccg
    41401 agtactacat ggaccggcac ctggagaagg ttttcaagat caaggacgag cgccagaaga
    41461 tcgacctcga cgacatccgt cgcctgtcga aggaggccga cgcgctgggc cttgcccgct
    41521 tcgtcatcac cggcggcgaa cccctggtga tgaaggactt cgacgccgtc gtcgaggcca
    41581 tcgacccgga acgccactac gtcatcaccg acaccaacgg ctggttcctc gacctggccc
    41641 gggccaggca catcaagtcc gtcggcgtgg agaaggtcca gctctccctc gacagcatgg
    41701 tcgaagccga gcacgacgcc ttccgcaaca agcccggctc ctacaagcgg gtcatgcgcg
    41761 ccatcgacgc cgcccaggag gccggcctca acctcatcct gtccaccgtg ctggtgcgcg
    41821 gccgtgcccg caccgacgaa ttccgccaga tgtgcgactt cgccaaagcc cgcggcatcg
    41881 gcctgtacgt ctcctacgcc aagcccaccg gctcgtgcac caaccacccg gaattcgtca
    41941 tcgacaagga agacgccgac atcgtccgcg agctcgaaaa gacccatccg gtgttcaccc
    42001 acatgacgcc ctcttacggc tcgttcaagg gctgcatcac cgtcaagggc atcatcacgg
    42061 tcacctccac cgccgaagtc accccctgtc cctacatcga cttctccctc ggcaacctgc
    42121 gccagacgcc gctcaaggag attctcgacc gcggcatgcg caacccctgg ctgggcccgc
    42181 accgtccgga ctgcctgatc ggcgaagacc cgcagttcat ccgcatccac accgaacgca
    42241 ccaaaggcgc cgtgaccctg cccctgccct ggggccaggg attttccgac cacgactcgc
    42301 tgcaggacga acaatgaaca ccgccaccct cgcccccgcc gccttcgacg acttcggccg
    42361 ctgcctgccc ctcggcgtca gcgccccggc caacgcccgc tcgcggcgct atttccgcct
    42421 cggcacgccg gccctcgacc cggccgccat ccatgcccgc ctcgcccgcc acctgggcgc
    42481 cggcaaggac atgagcaccg acaccttcat ccagcgcatc gccgccctgc gcgtccgcct
    42541 ggccgccgac cccgccaccg ccggcctgct ggacggtccc gcggttccct tcatcctgcc
    42601 gcgcacgccg gtcacggaga tcggcaccct gctcgaagaa ggctatctgc cggccgtcgc
    42661 ccgctccttc gaggaggcct tccccaacgc ccgcttcacc gaccacgccc accagggcct
    42721 gggcggcaag ctctcggtgc agcccggctc ccgtcacgag atcctggtgg acgccctcgc
    42781 ccacggcgaa gtagtcggcc tgtacttccc ctgcctcacc gaatattccc tgccggctgc
    42841 cgtcgagcgc atggccggcc tgcccgaaca cctgctcctg gcgggcggct tcgacacggc
    42901 cgccgccttc attgccgcac cggacctgct cctgcgcacc gacggctacc cgccgctgct
    42961 gtggctggcc ggcctgcagg gcgaaaagcc cggcatcggc taccacttcg aggcctacgg
    43021 ttacaacctc accttcaacc gccgccccca cctggaccag acggccgaat actggtggag
    43081 cggcgtgacc gtcctcgcgc ccgcctgatt cgcccttgac cgcccgtgtc atgcgggccc
    43141 cagtgctccc agcccggcag gacgccatgc gctcactcta tccgcctccc cgtcacccct
    43201 attacatcgt cgcgccggac taccggcgga cgtctgccgg cattcgtgtc atgcaccagc
    43261 tgtgccacgc cctgcggcag agcggcgaag atgcctggat ccagaccggg gtgacgcatc
    43321 ccaccctcga caccccacgg ctcaccgatg aggtgcgcgc ggcccatcgg gcccgcggcc
    43381 tgacgcccat cgccatctat cccgaggtgg tggccgccaa cccgctggac tgcgcggtgg
    43441 tggtgcgcta catcctcaac cgcccgggcc tgctcggtga acagccggcg tacgggccgg
    43501 acgacatcta ttacgtctac gaaccggagc tgctcgacat cgtgcccggc gccaccgacg
    43561 tgctgcgcct gccgtccatc aacacgtccc tgttcaacaa ccgcgacaat ccccacgacg
    43621 cccaccgcag cggaagctgc atctacttcg gccgctacag cgcgggccag caggaacacc
    43681 ctgacctggc ggacacgtgc accgtcatca cccaggactt tcccgccacc cacgaagagc
    43741 tggccgagct cctgcggcgc agcgagcggg tctactgctt cgaaaacacc gcgatctcca
    43801 ccgaagcacg cctgtgcggc tgcccggtgg tcatgctccc gagccccttc ttcagcaagg
    43861 aaaagttctt tggccgcggt cagggattcg atcaaggcgt cgccttcacc cagtcacccg
    43921 aggatctggc cacggcggcc cgcacggtgg gccacatcgc gacgctctac gaatccctgg
    43981 aggccgcctt ctggcctgcg ctcgacggtc tgatcaccct gacccaggcg cgcgccgacg
    44041 cggcccccaa gcccgacccc acgacccgca gcagccgacg tcccacccag gacgacaccg
    44101 aatacgaaac ctggcgtgcc cggcagaccc tccaggaaat cgacgcccag atcctggccg
    44161 agcgcatgat gctcacctgg cagcagcgcc cgggcatcca cctcctgctg gccctgcatg
    44221 cgggcgagga gcacctcctc gccgacaccc tggacagcct cgcggcccag ctctatcccg
    44281 aatggcgcct cacggtggcc accgccctcg acatgcctga gggcgtcggc gacatctcca
    44341 atctgcaatg gctcgccctg cgcgatgccg cgcacatcga ctacgtcatc gacgagatgg
    44401 cggccgcctc gcccggcacc tggctggccc gcattgagcc cggcctcacc cttgaacccc
    44461 acgcgctgca ggtcatcgcc gattacatca atgtccgccc cgactggcgt ctcatctact
    44521 gcgacgaaga caccctcgag gcggacggcc gctacacgca cccgctcttc aagcccgact
    44581 tcaacctgga tctgctgctt gcccaggcct atgtcggcag tctgatcgtc gtcgagaagc
    44641 aggccttcct ggccgccggc cgtttcggca ccctggccgg cgccgaaaac tacgatctca
    44701 ccctgcgcat cctcgatcac gccggcgaag ccgccatcgg tcacctggac cagttgctcg
    44761 cccatctgcc gcgggcctcc gtgcgcgcca tgcagcccga agcggaaaag gccgccgtcc
    44821 aggcccacct gctccgccgt ggcctcgatg ccgaagtcct cgacggcgcc ggctacggca
    44881 cgcggcgcgt catctgccgc tgggcggaat cccccctcgt cagcatcatc atccccaccc
    44941 gggacaaggt cgaatacctg cgccccttca tcgagagcct ggaagagcgc acgcgctacc
    45001 gcccctatga actgatcatc gtcgacaacg acagcgtcga tccggacacg ctggactatc
    45061 tcgccgccct cgccggtcgc cacgcccggg tgcgcacccg gatcctgcgc cagccgggcg
    45121 aattcagctg gtcggcctgc gccaatgccg gcgcggcggt ggccgaaggc gacttcctgc
    45181 tgttcctcga caacgacctc cacatcgtcc aggacgactg gctcgaccgc ctcgtggcca
    45241 ttgcccagcg cccggaagtc ggcatcgtgg cgccccgcct gacctacccg gaaaccgcca
    45301 aggttcagca gggcgtgtgg atcctcggca tgcacggcac caccggcaac ccctgggaca
    45361 accagctcgg actgaccgac cccggctaca tgggccgcgc cctgtgcgac cagaacgtca
    45421 gcgcggcaag cggctccgcg ctgctggtga aacgcgccgt gctcacgtca ctgaacggct
    45481 tcgatgcgga acggtttccg ctcctctacg gcgccctcga cctgtgcctg cgggccacag
    45541 cccagggtca caagattgtc tggacgccct acgccgcgct ggttcactac gcaggcgcct
    45601 ccgtcgtcgc gcgccagcgc cgcctcgaag gcaagctcac cgacctgatc gccgcccagg
    45661 aggcaaacca gaccctgctc gcgcgatgga tgccacgcct ggtcagcgat ccggcctata
    45721 accgccacct cagcctgtcc ttccccttca agccggaaca cgtagccccc atggactggg
    45781 atccccactt ccatgaccgc ccgcgcatcc tcgcctgccc ggtgcccggc ggtgccgggg
    45841 aattccggct gcgcgcgccc ctccgtgtca tcggtcaggc cggcctcgcc cagaccatga
    45901 tctgcgagcc gcccaagccg ttcacgatgc gcatcctgtc gcccgtcgag gtggcccgag
    45961 ccgatccaga cgtcctgatc ctccaccagc ccctcgaaga cacccagagc gacgccctcg
    46021 aacactacgc gcgctttctg cccaaggtcc gccgaatcct cacgatggat gacctggtca
    46081 ccaacctgcc gcgcaagcat ccgctctaca agtccggcta caaggatggg cgccagcgcc
    46141 tgcgccgggt gctgtccttc atggaccgcc tggtggtcag cacccagccg ctggccgatc
    46201 tgtgcgccga catgatcgac gacatcctcg tcatgcccaa cagcctcgag tggtccctgt
    46261 ggggcgacgt ggcgcccccc ggccggcctc gcacgaagcc ccgcgtgggc tgggccggcg
    46321 cccagcagca ccagggagac ctggagctca tctacaaggt ggtcgaagcc ctggccgacg
    46381 aggtcgactg gatcttcatg ggcatgtgcc cggaacccat tcgcggcttc gtccacgaag
    46441 cccatgggtt cgagctggat tttcgggcct acccccgcgc cctgagccgc ctcgatctgg
    46501 atctggccat cgcccccctc gacgtccatc ccttcaacga ggccaagagc aacctgcgcc
    46561 tgcttgaata cggcgcaatg ggctggccgg tgatctgcac cgacatctac ccgtaccaga
    46621 atgcaccggt cacccgcctt cccaacgacc cggacctctg gatccggacc atccgggaac
    46681 agattgcgga acccgccgcc ctcaaggccg cgggcctcgc cctgcagcgc tgggttcgcg
    46741 agggctatgt cctcgagaac cacacggcac gctggtttgc cgcctacgga ccctgaagcc
    46801 tcacgtgcat gtgtcctgaa agcctgtgat caacccgtgg aaaacccacg ctggcgcgtg
    46861 ctttgcacgc taaagcttcc tcggaatgcg ccgctatcac aagccaagcg gtaatccgcc
    46921 gagaacaccg caaaacacat caaattgtaa aagtcaagaa atttctaaag tatttccttg
    46981 tggccccgtt aatcaactca acggcggttg ccttacacac accgggcaag gcgaaccaaa
    47041 gttaagccta aacgcttatc ttgaattgaa cggaattaag gagaaccaaa atggctgcca
    47101 caatcaacac gaattccatg tcgctgaatg cgcagcggaa tctttccatg tcccaatcca
    47161 gtctggcgac atcggttcag cgcctgtcct ccggcctgcg cgtgaatacg tccaaggacg
    47221 acgccgccgg tctcgccatt gccgaccgca tgaacgcgca ggccaagggc ttcgacgtgg
    47281 cgatccgcaa tgccaacgac ggcatctcca tgggccagac ggctgacagc gccatgcagg
    47341 cgatcaccga caacctgcaa cggatgcgcg aactcgcggt ccagtccgcc aacggtacct
    47401 acaccagcgg cgaccggacc aacctggatg ccgaattcca gcagctgaac caggaaatcg
    47461 cgcgggttgt ctccggcacc cagttcaacg gtcagaacat cctgaccttc accgccagct
    47521 tcaccttcca ggtcggcgcg ggcaccgcca gcacggacca gatttcggtg accacggcag
    47581 gctccctggc gtcggcgacc ttcggcacca ccacggccgg cgccctgagc ggtagcagca
    47641 tctcgggttc gtcctcttcg ggcgccaccg ctgcgatctc cgccatcgac gccgccatcg
    47701 actcgctgaa cacctcccgc gccacctggg gtgccgtgca gaaccgcttc agttccgtcg
    47761 tgcagaccct gtccgttgcg tcggaaaacg ccaagtcgtc ccgcggccgc atcatggacg
    47821 ccgacttcgc ccaggaaacc gccaacatga cccgcgccca gatcctgcaa caggccggta
    47881 cggcgatggt ggcccaggcg aacagcatgc ccaacaacgt gctgtcgctg ctcaagtaag
    47941 cgcggaattg aaattcgccg cagcgggcgc cgccgccgga accggcgcgg cgccgtctgc
    48001 acatctcggg ggaaaagatc atgagcatcc aaccggtcgg cggggccagc acccagcctt
    48061 acacgcctcc cgtccagtcg acacccggcg cccagggggc gatccggaag gcggacgccc
    48121 ccaccgcccc cggcgacgtc caggctgtca gggacgccac ccagaacccc gccgcgccgc
    48181 cgaccccgga gcagctcaat caggccgtca aacagatcca ggacgtcatc aagcagaccg
    48241 cccagagcct ccagttctcc atcgacaaag acagcggcat gaccgtcgtc aaggtggtcg
    48301 acaccgagtc gaagaaggtg atccgccaga tcccgtccga ggaagtcatg gccatcgcca
    48361 aggccctgga caaactgcag ggcctgctgg tcaagcaaca ggcctgaggc atccacctcc
    48421 gcgccgggca tcgccgatcc caccctcgcg gtgggatttt ttcgcgggtt cgtgcatctg
    48481 gcatggagcc tgcttcttct cgactggtgc gtttccgctc aagtttcagc ctcgcgggac
    48541 gtaagagaaa cagcgtgttc acaggcatta cagggggcca tcatggctag catctcctct
    48601 gcagggctcg gctccggact cgacgtcaac tcgatcgtca cgtcgctcat gtcggtcgaa
    48661 aagcagccgc tcacgcagct ccagaccctg cagaacaaag accagtcgaa gatctccgcc
    48721 atcggcaccc tcaagagcaa tctggcaacg ctgcagagcg ccgcagcagc gctcaccccg
    48781 gcaatcgggc agtccccgac ggcagcattt gccgcctata cggcaacact tgccgacacc
    48841 acgataggat ctgccaccgc caccgcttcc gccgtggccg gcacctactc cgtcaacgtc
    48901 acctcactcg ccaccaatca gcagctggcc ctgggcaaaa cctacgcatc gaccgacccg
    48961 gtcatcgatt tcggcagtgc cagcaccaaa acgctgacct tcaccgtcgg ctcgacggcc
    49021 accaacgtca ccctgaacag cagcgccaac acgctggccg gcgtccgcga cgcgatcaac
    49081 agcgccaacg ccggtgtcgg cgcaaccatc gtcacgggca cagacggcaa gcagaacctg
    49141 ctgctctccg ccaccaacgg cggcaccgcc aacgcagtca cgatctccgg tgacgccagc
    49201 ttcatcgacg cgtcggcccc cggctcgccg attgccgcgg ccagcgcctt cacccagacc
    49261 caggcggcca ccgacgcggt cgtgaagatc cagggcgtgt cgatcgccac cagcggcaac
    49321 acggtcacca atgccatcga cggggtcacc ctcaacctca ccaagacagg cagcaccacc
    49381 ctgacggtcg cacgcaacaa cagcgacctg cagggcaagg tcaccgcctt cgtcaacgcc
    49441 tacaacagcc tcaacagctc catcaagagc ctcggcgcct acgatgccac caccaagtcc
    49501 gccgccatcc tcaatggcga ctcgtccctg cgcagcatcc agagccaggt ccgcgccgcc
    49561 ctcacgggca caccgaccct ctcgtcgggc aacgccaaca tcaaactgct gagcgacatc
    49621 ggtgtcagct tccagcgcga tggcagcctg gccttcgaca ccgccaaatt cgcgacggcg
    49681 gccagcaagg actacgtggc gaccgcaacc gtcatcgcca attacggcag cgcgctgaag
    49741 accacaacca ccaacctgct gggcaccaac ggcgtgatcg catcgcgcac caatgggctg
    49801 aacgcctcca tcaagagcat cggcaaccag atcgattcga tgaacgtacg cctgacgcgg
    49861 atcgaagcca actatcgatc ccaattcacg gcgctcgaca cggccatgag cagcatgacc
    49921 accaccagca cctatctcac ccagcagctc accctgctgg ccaatcagag caaataagcg
    49981 catgcgcgca ccccgccagc ccgaacggag attgtgatgt cctttggcct cgctgcccgc
    50041 agagccgacg cctacgcgag cgtcggcgtg gaaaccggag tttccacagc agacccccac
    50101 cagctcatcc tcatgctttt cgacggcgcc ctgcttcagg ttggcgccgc cgctgcggcc
    50161 atggaatccg gcaatgtacc gggcaagggc gcagccgttt cgaaagccat cgagatcatc
    50221 accaacggcc tcaaggtcag cctggactat gaagccggcg gcgaactggc cgcccgcctg
    50281 ggcgccctct acgactacat gacccatcgt ctcctctacg ccaacctgca caacagccgg
    50341 ccggcgctgg acgaggtcac caagctcctg tccgagctca agagcgcgtg ggccgagatc
    50401 ggccccggca cggacggcag catgtaagca ccgcttcagg attcatgttt acaattccac
    50461 ccgtcagcag cgatctccag accggctccg gagagccgac cggcaggatc gcgtgacccc
    50521 ggagacccca ccccatgagt tcccttgcac tcctggaaga aatgagcacc tactccaacg
    50581 agatggtgga tgccgcccgg cacaacgact gggaccggct cacccgcctc gaaaaaaagg
    50641 ttgcgtccct gcgggaccgc atcggcgtcg aggaggccct gggctccccg tcccggcgtc
    50701 gcatgtccga gtccgagcgt agccggcgca tcgacctgat ccggcgcatc ctcgacgacg
    50761 acaaggagat ccgcgtccac accgagccct ggctcgacaa cgtccgccag atgctgaccg
    50821 ggcaatcccg cgaacgggct gtccgcgccg cctacgacaa gctcgaccgc tgacgcggtt
    50881 cacagggcgg cgcgcagggt cccgtccgtc atcgctccct cggcgccatg atccccagcg
    50941 acctcgccgc ccgcttgcgg atgctcacgg aggcgagctt cttctccggc gagcccacgg
    51001 tggcgccgct cgcacgtgtc cgtgaggtgc cggccaatct gcccgatttc ctgccgggtc
    51061 agcgcatcgt ggcccgcctc cagcagagcg gtcccgacaa caccttcaag gccctcgtcg
    51121 agggccgcag cgtcaccctc gccctgcccc agaccgccac gcccggcgac actctggaac
    51181 tcgtggtcag tcacgtcacg ccggccgccg tgttcgccac ccaggcaggc agcaccgccc
    51241 tgcccggcgc ccagaccggc accgccctga gccaggccgg gcgcctcatc agctttctcc
    51301 tgaccggtca gccacccgcc caggccgcca gcctcaatgg cggccaaccc gtgctggcct
    51361 cgcctccggg gcccggtgac accgacctgc tcgcccgacg tctgggcgca gcgcttggcg
    51421 acagcggcct gttctatgaa tcccaccagg cgcgctggct cgccggccag gtcgacatgg
    51481 cccagttgac gcgggagccc caggggcggc cggcggcccc gtccgctgcg cccaccacgg
    51541 ccttgcagac aggcaccgcc gccctccccg aagccgccca gcccggcgcc accacccaga
    51601 tccccgaccg tctgctcccg gtggtccatc agcaactcga tgccctcgcc acccagcacc
    51661 tcgtctggca gggacagatc tggccaggtc agaccatgga atgggaaatt gaggaccccg
    51721 cccgcgatgg cccccgtgcc gacgacgacc cggcgcccac ctggaacacc accctgcgcc
    51781 tcagcctgcc caccctgggt ggcgtggagg cccgcctgca cctcacccct gccggggtcg
    51841 cactgcgcct gacaggtgac agcgccgaca cggtcgcggc cctgcgtgcc ggacagccgc
    51901 gccttgccga agcccttgcc gccgccaacc tgcctctgac cggcgtctcg accgaacagc
    51961 gggaaccggc atgaacggcc ccgcccagcc cgaaatcccc cgcgccgaag ccgttgccct
    52021 ggcgtacaac gccggcaatg gtgcgccaag tgtcgtggcc aagggacgcg gcctgatcgc
    52081 cggagaaatc atcgcccgcg cccgcgaagc cggcgtctat gtgcatgaaa gcccggaact
    52141 ggtcgccctg ctgatgcagg tggacctgga cgcccgcatt ccgcctgaac tttacgtggc
    52201 ggtcgcggaa ctgcttgcct ggctgtacag cctcgaagac cacgcccgcc accaaaaaga
    52261 aacggccccg cagggccgtc cgttgccgta aaccgccgga ttcagggctt gggcggcacg
    52321 taaccaccga tctggtccgc cccgccggcg aagaaatagc gctccatctg ctccgcaagg
    52381 tacttgcggg cccgcgtgtc catcagattg agacgattct cattgatcag catggtctga
    52441 tgccgcaccc attgctgcca gccttccttg gaaacgttct cgtacacgcg cttgcccaga
    52501 tcaccgggca atggcggcat atccagccct tcggcctcac ggccgagctt cacacaattg
    52561 accatcctga ccatgcatca cctctcagtt aggaatccgg caccgccaca cgcgcgccgg
    52621 cttgccggac gattctagcc caagcccggc tcagcgccca cttcaggatc attgccctcc
    52681 gccgccgctg ccacctccga gcgattgccg ccctgcgcct tggcccggta catggcccgg
    52741 tccgccgccc cgaccagcgc ctcggcgtcg gtgccgctga gtccgctcca ggcaatgccg
    52801 atgctggcgg tgacacccac cgcgctgcca ccgaaatcga accgcagggc tgcgatgcac
    52861 gctcccaccc gccgcgccag cccggccagt ccctgcacat cggccgaggg caccaggatc
    52921 gcgaactcgt ctccacccag acggaagaac atctcgttgc gcctcaccac ggcggcaacc
    52981 gcctgggcga gcccggtcag cacctggtcg cccgcctgat ggccaaaggc gtcgttgacc
    53041 ggcttgaagc cgtccagatc cagcaccagc agccccacat catcgccgcg gcgacgggcg
    53101 tcgccaagca tccgctcgag ctcttcatga aaacggcggc gattgtagag gttggtcagg
    53161 gggtcgcgtt cggcgagatt gagcagttgc aaggccacgc gacggcgctc cgtcacgtcc
    53221 tcatacaccc agatgcgacc gatcgcccgc gccccgtcgt ccccgaacac cacacgggag
    53281 aattcattga ccgtccgtcc gtcgctgaaa tgcagctcga agggtgcact ggcctcacgg
    53341 gagccgagca cgccggccac gtggcgcaga tagcccggcg gatcggtcag cagggtcacc
    53401 gcatgctgct gcaggaccac gtcgcgcaca ccgacgatgc tctcgccctc ggccactttc
    53461 cagatctcgc acagtgcccg gttgcaatac accacgcggt ggtccggatc catgaacagg
    53521 atgcccgcct ccaccacgtc gagcagggcc gacaggcgcg agcgttcttc gttgctgcgg
    53581 cgcagatagt gctcggacag agcctgggcg cgacgcaggg ccgcaacggt ctcaaggagc
    53641 ttgaactccg cctccttgcg cgcctggatg tcgtcaccgg tggcacggat gccctgcacc
    53701 acgcccgcct cgtcccgcac cgcgtgccag tggcaggtca cccagcgcag ggcgccctcc
    53761 cgcgtcagca cgcgggtctc gaaatcggcc ggccggccgg tgctgagcac ctgggtggtc
    53821 tgctcgcgca catagcgacg gtcccgctca tagatgatca gttcgacgaa gtcgggcgcc
    53881 gccagcacat cctccaccgc atggccggca aagcgctcga aggccgggct gacccagtag
    53941 agacgccccc gcgtgtcgat cagcccctcc agcacatggc ccgagtccgc cgccagcgca
    54001 aagtaatgct cccacggtcc ggcggcaggg gacgccaccg ggagtcccgt gcgggacgcc
    54061 ggctcaaccg gcacaggcgg gtccaggcgg gtcgcttcca gcgcgcggat acgccgccac
    54121 aggacgcacg cccacagagc cgcggcagcc agcgccggca ccaccgcgag cgccggcagc
    54181 gagccaccgg gccaggcctc tgcggccgtc gcaccccccg cccacaagga cggaacgaca
    54241 gcggccccga cctgccgcca gcggagggcg cagatccggg aacagatggc agacatgacc
    54301 gatccgtgcc tcagagtgcc ttcatgaaca ccttggaacg gcgttccacg tcgtacattt
    54361 cacgcttctg acgcggcagt tcctccggtg gcatggcacg gaagccccgc tcccggaacc
    54421 agtcggaggt gcgcgtggtg agcacgaaca ggcgctcgac gcccagcgcc cgggcgcgag
    54481 cctcgatgcg cttgagcagc atctcgccca gcccggcgcg gcgatgctcg ggcaccaccg
    54541 ccacgcaggc catctcggcc gcccggtcgt cgatgaaggg atagagtgcg gcacacccca
    54601 ccagcacccc gtcgtattcc acgaccgtga agcgctcgat ttcctgctcc aggcgttcgc
    54661 ggctgcgacg caccagggtg ccgtcggcct ccatcggcgc gatcatgctg cccagggccc
    54721 ccacgtcctc gatggtcgcc tcgcgcaggc ggaagagggg atcgcgcgtc agcacggtgc
    54781 cgacgccctg gggcgtgaag aactccagca gcaggccgcc gtccttgtcc cggtcgatca
    54841 gatgggcacg cccgacgccc ttgcgcaccg cacggatggc gcagggcagg tagaggtcca
    54901 gatcctcggt gaggcccttg ccggccttca gcatggcctc cgcgtcgtcc gcggtgatcg
    54961 cctcgatgag cccgccgccc tcgtccagca gccccggcgc atcgcacaga tagatcagtt
    55021 tttcggcctt gagggcgatg gcgacggcct cggccaccgc ctccatgctc atgttgaaga
    55081 tctcccccgc cggcgacacg cccagcggtg acatgagcac cacgttctgc tgatccaggt
    55141 cggcctcgat ctcctcggca atcaccttgc gcacggcacc ggtgaaaccg taatcgaccc
    55201 catccaccac ccccacaggc ttggcggtga cgaagttgcc gccggtcacg cgcatgaagc
    55261 tgccggccat gggcgtgttg ggcaggccct gggagagcag cgcctccacc tcgatgcggg
    55321 tcaccgccat ggccgccttc acgcattcga tggcgtccgc atcggtgacg cgcaggccct
    55381 tgtggtagcg gggctgcaag ccgcgacggg ccagctccgc ctcgatctgc gggcgcgcgc
    55441 catggaccag caccagacgc acgccgaggg ccgccagcag gttgcagtcg taggccaggg
    55501 tctgggccag gctgcccgcc gccacctcgc caccgaaacc cagtacgaag gtcttgccgc
    55561 ggaacgcgtg gatgtagggc gcagctgcgc gcacccaggc gacgaagcgt tgcgagaggc
    55621 cgggatcggg gacggcggcg ggaacggatt ggggcactgt cacgggcatt ccagaaggca
    55681 gaacagggag gcggaccgtg tcctggggat gcacggcggg ctgaccatca ttgtagccgc
    55741 gccgtcaggc cttcaggctg cgttccacgg tctcgacgat ggtgcgcagg atcttcaggc
    55801 gcgcaaagta cttgttgtcg gcctccacca gggtccacgg cgcgttctca gtgctggtgc
    55861 ggtcgatcat gtcgcacacc gccggctcgt agagcggcca cttctcccgg ttgcgccaat
    55921 cgtccggggt gatcttgtag cgcttgaagg ccgcctcctc gcgggccttg aagcggcgca
    55981 gctgctcgtc ggcgctgatc gacagccaga acttcacgac cagggcgccg tgactgacga
    56041 gctggtcctc gaagtcgttg atctcggcgt aggcacgcat ccagtcggcc tccgtgcaga
    56101 agccctcgac ccgctccacc agcacgcggc cgtaccagga acggtcgaag atcgccaccc
    56161 tgccgcgccg cggcaggcgc cgccagaagc gccacaggta gggctgggcg cgttcctcgt
    56221 cgctgggcgc ggcgatgggg atcacgtggt actgacgggt gtcgagcgcc tgcgtcacac
    56281 ggcggatcgc cccccccttg ccagccgcgt ccatgccctc gaagaccagc accagactgc
    56341 gctgggtaaa gccgcgccgg cgcagggcca gggacagacg tccctgcagg gcctcgcgct
    56401 gggcctcgta ggccttgcgc tccatgggct gatccagcgc cagggcgttg agcagggtgc
    56461 gccggtcggc ggccgccacc aagggcgcgc tgaccaagcg ggcgcgatag cccttgcgca
    56521 cgtgctccag gtgggcctgc agacggtgca gcagggtccg cccgacgaac accgcccgat
    56581 attggggatc ggacccgtcc acgatcaccc acggggaatg ggccgtactg gtccgtcgca
    56641 gcacgtgctc ggcggtcgcc cggtagcggt cgtaatggtc atagaccagc cggtccgagg
    56701 cgcgcacccg ccagcgggtc gccgggttgg cctccagggc cttgaggcgc ttcttctggc
    56761 cgtcccgcga caggtggaac cagaacttga ccagcaccac gccctcggcc gccagcatgg
    56821 cctcgaagcg gttgaactcc gacaggcgct ggtcgagccg ggccttcttg atgcgcccga
    56881 aggcgcgatc gccgaggggc tcggaatacc agttgttgaa caggatcccg atcttgccgc
    56941 ggggcggcag agcgcgccag aagcgccaca ggaaaggccg ctcgcgctct tcgtcggtgg
    57001 gccgatcgaa ggcgtggacc tcgatgtggc gcgggtccat ccattcgttg agcagattca
    57061 ccgtctcgcc acgcccggcg ccgtcaatgc cgttgatcag caccaccgcg gcaaaatcct
    57121 tcttgtcgag cagatcgagc tgggtttcga ggagctcggt acgcagcacc ggctcgatct
    57181 cggcgtactc ggccttggac agcttgtgtc ccagctccgc ggattcgaac atctcagcct
    57241 cccggaaaat cgccccacag ggtctgcagc gctgccagcg ccgccaggcc cgccgtctcg
    57301 ctacggaaga cccgcggccc cagggccagc ggctcgcagc cagcgcgcat cgcaagcccc
    57361 agttcggccg gcgaccagcc cccttccggc cccaccagca cggccagcgg gccgtccggc
    57421 ggcgccatgc gcacgagggc gcctgcgtcc cctggcgcca gaatcagacg ccgccccgcg
    57481 aacggcgtac ccagccagcg cgccaggggc aggatctccg ccacctccgg cacccggttg
    57541 cgtcccgact gctcacaggc cgacacgacg atctgctgcc agtgggcgac gcgtttggcc
    57601 gcccgctcgc cggccagctt gagcaccgag cgctccgccg ccaccggctg gatcgccgcg
    57661 acacccagct ccaccgcctt ctgcaccacc cagtccatct tgtcgccggc cgccagggcc
    57721 tgcacgaggg tgatcgccag gggcgactcc cgctccaccg cacgccgcgg cccgaggcgc
    57781 gccagcggct gacgtcccag cgcgacgagc acggcatcgc actcgccgcc acggccgtcg
    57841 aagagcgtca cgccttcgcc gaccgccagc cgcagcaccc gttcggcatg atgggcgacg
    57901 gccgcgggca ggatcatctc ggagtcgggc tgcagcccgt cggggcagaa gaagcgcgga
    57961 ttcatcatgc tattatccgc aaatccacgc cccttcacgg gattgaaacg aagaacatgg
    58021 tcagcctctc ccctcccgtc tgcgatttcg gctggtcggc ccccgatttc gccctgccgg
    58081 gcgtggatgg ccgcacccac agcctcgctt cctgccgcgg cccggccggg ctgctggtga
    58141 tgttcatctg caaccactgc ccctacgtga aggccatcct gccgcgcctg ttgcgcgact
    58201 gccgtgagct tgcaggcctg ggcgtcggca gcgtcgccct catggccaac gatccggccg
    58261 actaccccga ggactcctgg gaagccatgc agcgcatcgc cgtcgaacag gatttccctt
    58321 ttccctacct cctcgacgcc tcgcaggacg tggcgcgggc ctacggtgcc gtgtgtacgc
    58381 cggacttctt cggcttcaac cgggatctga agctccagta ccggggccgc ctcgacgcct
    58441 cgggcaggca gcccgccccg tccgatgcac gccgcgaact cttcgaagcc atggcgcagg
    58501 tggcccgcag cggcgaaggc ccgcgggacc agaccgcaag catcggctgt tccatcaagt
    58561 ggaggccgag cgccccgtga ccagcaccca acgcctcgaa catgcccgcg ccctggccga
    58621 tctcgtccgc gcgatggccc gtgacgtgat tctgccgcgc tacctgcgcg ccgtgcgtga
    58681 acacaaggcc gacggcagcg tgctcaccga agccgatctg gccgcccagg cggccctgga
    58741 ggagcgcctg ccccacctca tcgccgcgcc ggtactgggc gaggagatga ccgccgagga
    58801 acagggccgc gtgtggcacg cgggcaaggc cggcctgtgg gtgatcgacc ccatcgacgg
    58861 caccaccaac ttcgccaacg gcattccctt cttcggcgtc gcggtggcct atctggtgga
    58921 ccacaagacc cagctgggcg tcgtgtacaa cccggtgacg gacgaagcct tttacgccgg
    58981 gcgcggcgcc ggcgcttggc tcaacgacgt gtccctgccc ctgcgccagc ccaccgagcg
    59041 ccttaaggac gccgtcgccg gcgtggactt caagcgcatc agccatcacc tgggtgacga
    59101 actggccgta cgcccgccgt attattccca gcgcaatttc ggctccagcg ccctggaatg
    59161 gtgctacgtg gccgccggcc ggctcgacgt gtacattcac ggcggccaga tgctgtggga
    59221 ttacgcggcc ggccgcctga tcctcgaaga agccggcggc tgctacagtt ctctgggcgg
    59281 cacgccgctg accgccgggc cggccatcaa gcgctcggtg atctccgccg ccagcccggc
    59341 cctgttcgaa caatggcgga cctggctgca cgcccacgcc tgacatcggc acgacgcttg
    59401 cagggagtac ttttggaaca ggccatggcc ccgtgccctg ctccaaaact actcactttc
    59461 aggatgcgac catgtcgatg aagcacccca tcatcgtggt gaccggctcg tccggcgcag
    59521 gcaccactac ggtcaggcac acctttgagg ccatcttctg gcgcgagaag gtcaacgccg
    59581 ccatcatcga gggtgacagc taccaccgct acacccgaga agagatcagc gccctcatca
    59641 ccgacgcgga aaaccggggg gaacggggca tcagtcactt cggaccggaa gccaacctct
    59701 tcgaagaact ggaaaacctg ttccgcacct atggcgaatc cggctccggc cagcgccgcc
    59761 attacctcca cagcgtcgag gaagccgccc gccgcggcct caaggtgggc agcttcaccc
    59821 cctgggaaga cctgccaccg gacaccgact gcctgttcta tgaggggctg cacggcgcgg
    59881 tggtgaccga caaggtgaac acctgccgtc atgccgacct gctcatcggc gtcgccccca
    59941 tcaccaacct ggaatggatc cagaagatcc accgggacac gcgcacccgc ggctattcca
    60001 tggaagcggt gcaggacacc atcctgcggc gcatgcacga ctacgtgcat tacatcgtgc
    60061 cccagttcac ccacacccac atcaacttcc agcgggtgcc catggtggac acctccaacc
    60121 ccttcatcgc gcgggacatt cccaccgccg acgaatccat ggtgatcatc cgcttccgcg
    60181 acccccgcgg cgtggatttt ccctacctgc tgcgcatgat ccacgacagc ttcatgtccc
    60241 gcgccaacac catcgtggtg ccgggcggca agctggatct ggcgatgcag ctgatcatca
    60301 cccccctcat ctggaagctg atggaacgcc gccgccagtg ggtgtaaggc accggttgaa
    60361 gctcagcgcg gtgccgccgc cacggtgatg tcgcgccccg cgtcggtctt ctgatcgtcg
    60421 cggaactcgc cctcccagcc gccaccgacc gcccacatca ggcgtcccgg gccgtgcttg
    60481 cggcccaggt tccaggcccc ttcgtagcgg ttgccgttct tccaggcgta ggtgccctgg
    60541 ccgtggaaca ccccgcgcac gaagtcgccg gtgtagcggt cgcccgacgc gaagatcagt
    60601 gtgccgcgcc cctggggctc cccatccacc acgccgcctt cgtagcggtt gccgccgacg
    60661 aactggatca cgccctgacc ggtggcccga tcgtcctgcc actccccttc gtaccactgg
    60721 ccgccgaccc agtcgaagcg cccgcgaccc tgcttgcgtc cgcccaccag ggagccggtg
    60781 taacgctcgc cgttggccca ctccaccgag ccactgccgg acaccgcgcc agcggccgcc
    60841 tgatcggcga cgaaggcgcc ccggtaggtg gtatcgcccg ccacgatcac ggtgggctcg
    60901 gccggcgccg gcgcagcggc cggcttcgcc gccacgggct cgctgcgggt gtcgggcttc
    60961 gcgggtggcg gcgccacgac gacgggccga gcgggctcgt ccctgggtgc cgggcgcgtg
    61021 ggcagcggcg gcgttacggc gaggctcgcg acctgcgcag tcgcccccgc cgcacccgcc
    61081 acgggcaccg acgccggtgc gacggccgca gcgctcccgg ctaccggcag cttgcgcccg
    61141 tactgggcca tcgcattggg gggcagcggc gcgccgcgct cccgcgccag ggcatcggcg
    61201 ttggaacggg cgagattctg cggcgcgccg cggcactggg ccgcagcgtc gcgccaggcc
    61261 acctcggccc ggatcgagag ctggtcccgc tcggccaggg cggcgctggt cccggcggcc
    61321 ttgacgcgct gggactgctc ggtcgcggcg tcccacaggg ggccgcagaa ctccgcgttg
    61381 tgggcgtcgg tctgagtctg ttcgatcttg cgctgggcca gttgctgacg gttgccggaa
    61441 cacttgtcgg cggccccctc ccaggccagc gcagccttgc tgtagagcat gcccgcatcg
    61501 tcccagcgct tgtccttcca cgccgccttg gccaggtcca ccagggtcgt ggcgttcttc
    61561 cacgccccat cgcaggccgg cccggcgctg agcagctcgg cgttggcggc gcgggcacgc
    61621 accgtggcgg ccaggctgcg ctccgccttt tcccggtggg gcgacaggca caggctgacc
    61681 gcctcacgcc acagggcttc ggattcgccg agcagccgtt ccatctcggc cggatcgccg
    61741 ctcgcagact tcaccgatgc ggcgcggatg tccaggtcga gggccttgtc gaggcgcggc
    61801 tgacattcgc tgtggtggga catctgccct tccgccagct tgagctccgc caccgccttt
    61861 tcgccctgcc ggttggcctg gggcgccagg gccgggcagc cggcggctac cttgaggaag
    61921 ctggcagagg cctcccgcag caccgctgcc gcgtccttcc aggccctggc cgcgatgccg
    61981 gcgcgcccgc gctccagcgc cgcttcggcc ccctgcaaca ggatcggaca cgcgtcggcc
    62041 ggcgccgccg cagacggctc atcggccgcc ggcatggatt gagcctgggc aagggatccg
    62101 cccacgagca ttgcgaaggc gaaaggggta aggcgggtca tcagcggcat ggcagttccg
    62161 tcaatcgtcc ccggattcga ggggcatgcg cagcagggca tcgatcagtc cgcccgaagt
    62221 gccgccgggc ccctgctcgg ccatttcgga ggccatttcg agggctgcgg gctccgagga
    62281 ggactcgggc agatcggtca gcttcaacgt aaacgcctcc agcgacgcac ccttgagcac
    62341 cgccgcgagc atctcttcac gctgggtctg acgtgccagt gcggcgtagc gcaccaggcg
    62401 ttcctcttca agcacgaagg cgttctggat cagccccttg tccagcagct tctgatgcag
    62461 cgtggcgccg gccagggggt cgccgcccag cttggcgatc cggtcatagg cccagcggtc
    62521 ggcgtcgtcg aacaccgtgg tcgccaccca ggacaggctc gacaccgcca tgcccatgcg
    62581 tgccatgaaa ctgtcgcgca gcacgttgac gccctgtctg cccacggact tggccacctc
    62641 ctcgccgacc ttctgggcat agtccttggc ggcctgctga ctgatctgcc gggccgcctg
    62701 ctccagcgcc tgtctggctg cggcttcact cgccgacttg gcagccgctg tcgcggcagc
    62761 ggtggccgct tgattgaccg ccaccttggc cgcctgggcc gccgcattga ggccggtggc
    62821 gagggacgcg atctcgaaca gcgtggacac gatctccttg ctgtagtgat aggtcttcat
    62881 ccgcgcgccg ccctcttcgg acagcccctg agtccacaac accatccaca gggcctcgtc
    62941 cggcgtgacc ggcgggatga acacctgcag cgggtggatg ctcatcagcc agtggaaatc
    63001 ctgatagccg ggcgtggcgg gcggcgcgag gcgggtgtag ccgcggcaca cctgcacggg
    63061 tttgagggtg accgtccggc ccgccgccgt cttcaccgga aagggcttgc cgttgtcgcg
    63121 catctcggcc agctcgacca gcatcttgtt gctgtcctcg cgcttgtagc cgtcgatctc
    63181 gacgatcttg tcacccacct ggaaacctga gcccggcgcc gccgccacca ccgtgaggcg
    63241 ctcgtccacc tgcagtgccc gcacccaggc tacccggtca tcctccggcc attcatagct
    63301 ggtggccacg ctgacgggca gatcccactg gtggctgcag tccgggtcct tcaactcggc
    63361 ggagcgcacg atggccggac gcagggcgcg ctcgccgctg cccagcagac gcaggttggc
    63421 caggagcgcc tcgttgacct tgcgcccgtc atcgacggtg tatttcgtcg tgctgcaacc
    63481 gcctgccagc aacgcggcgg cgatcagggc caggagcggt cgccggccac gcggaaaagc
    63541 tgccatcgcg cgaagatcac aaaa
//
LOCUS       DAOQPA010000008        49377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_275_length_49377_cov_19.241697, whole genome shotgun sequence.
ACCESSION   DAOQPA010000008 DAOQPA010000000
VERSION     DAOQPA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 49377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49377
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_49377_cov_19.241697"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKX01_02250"
     CDS             complement(<1..349)
                     /locus_tag="PKX01_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HMZ74971.1"
                     /translation="MDIKAYRDIAWNGWWKQNPGIVQILGMCPTLAITTNIVNALSLG
                     LATMFVMGMSNLAVAALRNFIPYEIRIPVFILIIAALTTVVDLSMNAFVHDLYLVLGI
                     FIPLIVTNCIVLAR"
     gene            complement(363..1022)
                     /gene="rsxG"
                     /locus_tag="PKX01_02255"
     CDS             complement(363..1022)
                     /gene="rsxG"
                     /locus_tag="PKX01_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HMZ74972.1"
                     /translation="MSETTALGLSTRTGAVMIAFTVVFTALMAFTEDATRERIAASAN
                     DEKMKSIAEVLPPAAYDNALLDDFILTGPTPALGLDDGGRIYRARKAGQPAALVLEAI
                     APDGYSGKVNLIVAVRADGSLSGVRVTGHRETPGLGDYIDPKKDKNKAAPWIVQFTDL
                     NPASTPLDQWKVRKDGGQIAYHTGATISARAVTNAVARAARFAADNQNRLFAAPSGKP
                     L"
     gene            complement(1028..2050)
                     /locus_tag="PKX01_02260"
     CDS             complement(1028..2050)
                     /locus_tag="PKX01_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HMZ74973.1"
                     /translation="MLNSPFIRKPASVQSVMLQVLLALVPGIAAYVWFFGAGILVNLA
                     LATAAALAGEAALLTVRGRPRALFLTDLSAVVTAWLIALTFPPIVPWWITVLATLLAI
                     VVVKQLYGGLGQNPFNPAMAAFCLMIVAYPALMSQWPAPGQLDFTTQLDLIFGGARAA
                     DAITGATPLDALRTALKSAANPDSPLYNLPASQLMDGPAFGTLGGQGSEWVALGYLLG
                     GLWLIQRRIITWHIPFAFCAALFAVAGLLWAVKPAGFASPLFHLASGGSMLGAFFILT
                     DPVSGATTPRGKLLFAAGAACLTYIIRAFGAYPDGIAFATLLMNILVPLIDMKTQSPV
                     FGHKKD"
     gene            complement(2075..3781)
                     /gene="rsxC"
                     /locus_tag="PKX01_02265"
     CDS             complement(2075..3781)
                     /gene="rsxC"
                     /locus_tag="PKX01_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HMZ74974.1"
                     /translation="MLRKLFSFHGGVKPQTHKAQSTTAPIAPVPMPDRLVIPLHQAVG
                     GQPRPLVKPGERVFKGQRIGAADGNVSSAVHASTSGLVRDVAPALMPHTSGLSALSVI
                     LEPDGEDRWGELETINLKSAAPDELRDYLRDAGVVGLGGAVFPSHLKLKPGKVGHTKT
                     LVINGAECEPFITCDDLLMRERAGDMLKGISAMRAMLQAEEVLIGIEDNKPEAVAAVQ
                     AAIAARGEAGIEAVAIPTRYPAGGAKQLIRVLTGIEVPHGRRSTDYGVQCFNVGTAFA
                     IHEALDRARPLVSRVVTVTGNVAHPRNYDVLLGTPMDHVVRLAGPGADTDRYIMGGPM
                     MGLRMPSFDVPVVKATNCILVGSPALFPPRPPEMPCIRCGECSKACPADLAPFELYWF
                     SRSKNFGKAQEYHLFDCIECGCCSYVCPSQIPLVDYYRFAKSEIWARERDKDASDRAR
                     DRFEYKNLRQEREKQEKAEKLAAKAAATREQAAAKPAGDAPATGTAPAAKPSPDDAKR
                     ALIEAAMEKARQQKAQVTPKNTDHLSPGQQADIDAIDARRQAASDAPAAAKADEPPAG
                     SA"
     gene            complement(3830..4399)
                     /gene="rsxB"
                     /locus_tag="PKX01_02270"
     CDS             complement(3830..4399)
                     /gene="rsxB"
                     /locus_tag="PKX01_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HMZ74975.1"
                     /translation="MLSALLVMTAIALVLGAMLGFASIKFKVEGDPLVDQIDAILPQT
                     QCGQCGFPGCRPYATAIANGEAEINQCPPGGEEGIRKLSDLLGREFKPLSEEHGVEKP
                     KSVAVIDEHTCIGCTLCIQACPVDAIVGAAKQLHTVVARECTGCELCIAPCPVDCIHM
                     RPIAETVDTWKWRYPVIPLAEAAPADRAA"
     gene            complement(4411..4995)
                     /gene="rsxA"
                     /locus_tag="PKX01_02275"
     CDS             complement(4411..4995)
                     /gene="rsxA"
                     /locus_tag="PKX01_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HMZ74976.1"
                     /translation="MSSYLLVILGAVLVNNVVLVRILGLCPFMGVSKKLDTAVGMGAA
                     TTFVLTLGCGTSYLIDHYVLSAFGLSYLQTLAFIVIIAAIVQLTELIIQKTSPVLHQV
                     LGIYLPLITTNCAVLGVPLLNVALHHDLLQSLLFGFGSSVGFSLALILFAGIRERLDG
                     ADVPAPFRGTAIAFVTAGLMSLAFMGFAGLDKYH"
     gene            complement(5090..6103)
                     /locus_tag="PKX01_02280"
     CDS             complement(5090..6103)
                     /locus_tag="PKX01_02280"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMZ74977.1"
                     /translation="MLYDIARPVLFSFDAETAHEAALAALNVAGRVLPAGKPLDARPV
                     DVMGLRFPNRVGMAAGLDKNGEVIDGLARFGFGFIEIGTITPRPQAGNPKPRMFRLPE
                     VRGIINRMGFNNHGIDALVANVQAARYRGILGINIGKNADTPIERAADDYLACLDKAY
                     PLASYVTVNISSPNTKNLRQLQGESELDALLGQLKARQTALADRHGRYVPIALKIAPD
                     LDDAQILNIADALRRHRIDAVIATNTTLARDKVQGVPYGDQQGGLSGAPLFEASTAVV
                     AALARALQGELPIIAAGGIFDGAQALAKLQAGAQLVQVYSGLIYRGPALVRECVAATR
                     DAA"
     gene            complement(6136..6621)
                     /locus_tag="PKX01_02285"
     CDS             complement(6136..6621)
                     /locus_tag="PKX01_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74978.1"
                     /translation="MPHLPSAFVAATLLACAPALHAGDSLDTLADAFANAPHRDWLSV
                     SGASHHFQPHRRAWREFNPGVGFERSFNGTPWVASAGYFRNSYDRDTWYLGGRWMPLA
                     AGPFRFGAFGLLASGYPSPVLVLPAVSVEAGRVGANLVALPNLPGYSGYLGLQVRVAL
                     D"
     gene            complement(6629..7369)
                     /locus_tag="PKX01_02290"
     CDS             complement(6629..7369)
                     /locus_tag="PKX01_02290"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HMZ74979.1"
                     /translation="MLKDYPYSQLQFYATAPYACSYLSERQARSQVATPSHLIDAELY
                     SELVRSGFRRSGIFTYRPYCDNCRACVPVRIPVSGFRPTRSQRRAWKSHALLDPHELP
                     LEFDEEHYALYQRYQATRHAGGGMDQDNREQYAHFLLQSHVDSRLVEFRENGVLRMVC
                     VIDCLTDGLSSVYTFYDPDPSCAGLGTFGILWQIEVCRQLGLPYLYLGYWIRDSRKMA
                     YKARFRPIQGRVGGQWQILSDADLDLPE"
     gene            complement(7374..8078)
                     /gene="aat"
                     /locus_tag="PKX01_02295"
     CDS             complement(7374..8078)
                     /gene="aat"
                     /locus_tag="PKX01_02295"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMZ74980.1"
                     /translation="MIPWLGDHPAFPPVRRALRHPNGLLAAGGELTPPWLLEAYRHGI
                     FPWFNDGEPILWWSPDPRMVVFPSEARITRSLRKTLRKGAFEVRLDHDFSAVIRACAA
                     PREPDGRTWITPEIQAAYGRMHALGYAHSVETWIDGELAGGLYGMALGHVFFGESMFS
                     RRTDASKIAFAHLIRFLEQNDFRVLDCQMTTAHLASLGGREIPRAEFARLVDTHQRTG
                     PPPGRWPQDGAQAPWT"
     gene            complement(8124..8678)
                     /locus_tag="PKX01_02300"
     CDS             complement(8124..8678)
                     /locus_tag="PKX01_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ74981.1"
                     /translation="MPSKKTITGFFVAIAFSGAVFAAEPPAAADESQVSAFFERYSDS
                     LRAYTDSVKALMDRSLNFLGVPYRLGGTNPLTGFDCSGFVGKVFTDALGFGMPRTAAE
                     MAQMGEQVNRSDLKPGDLVFFNTMRRAFSHVGIYLGNNQFVHAPSAGGVVRVEDMRIA
                     YWAARYDGARRLLPSAQARQSQAN"
     gene            complement(8968..9798)
                     /gene="serB"
                     /locus_tag="PKX01_02305"
     CDS             complement(8968..9798)
                     /gene="serB"
                     /locus_tag="PKX01_02305"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HMZ74982.1"
                     /translation="MNLVVQGPKLEARALKDLSTLAGSQGLGRLGTNAFRLLDAHLTP
                     EVSAYCEAHGLDCAEVPEGRRLQDYRLFVTDMDSTLITIECIDEIADMHGLKAEVSAI
                     TEAAMRGELDFRASLSRRVALLEGLPEAALARVYQERLHLNPGAEALMTGLKRAGVVT
                     VLVSGGFTYFTDRLKARLGFDYAFANQLEVRSGRLTGRVKGEIIDGDAKRRALRLVRE
                     HHGFARNTVIAAGDGANDLAMLAEAGIGVAYHAKPVVRAQATHALSHAGLDGILHLFN
                     "
     gene            complement(9798..10871)
                     /locus_tag="PKX01_02310"
     CDS             complement(9798..10871)
                     /locus_tag="PKX01_02310"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HMZ74983.1"
                     /translation="MSSAQTDNLNIAAFDRMPSPDEITTRVPLSPAAEQTVLTGRRTL
                     QAILDHQDPRLFVVVGPCSIHDPVAGIDYARRLKTLADEVADTLVLVMRVYFEKPRTA
                     TGWKGYINDPYMDDSFRIEEGMEKARSFLLQVNQVGLPAATEALDPIAPQYYGDLISW
                     TAIGARTSESQTHREMASGLSTPVGFKNGTDGSLDAAVNGIISAANPHSFLGINGQGQ
                     SSIIRTRGNRYGHVVLRGGGGRPNYDTVSVSLAEKALAKAKIPANIVVDCSHANSWKN
                     PEYQPLVMRDVAHQIREGNRSIVGLMIESFIEAGNQPIPADLSTLRYGCSVTDACVSW
                     DTTVDMIRNTRDILRDVLPQRGA"
     gene            complement(10946..11587)
                     /locus_tag="PKX01_02315"
     CDS             complement(10946..11587)
                     /locus_tag="PKX01_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HMZ74984.1"
                     /translation="MKPVWLFDLDNTLHNASPHIFPHINRSMTAYLMRHLALDEAAAN
                     RLRVDYWRRYGATLTGLMRHHGTNPHHFLHDTHQFPELHRMVVADAALSHLLRRLPGR
                     KIVFSNGPRRYAEAVLDVLGVRHHFSDVCAVEQMRFHPKPDIRGFRHLLRQQRLQPAR
                     CILVEDSADNLRTARRLGMKTVWISRSLRRPAWVDVRLPSVLALARHPLRRGR"
     gene            complement(11604..12503)
                     /locus_tag="PKX01_02320"
     CDS             complement(11604..12503)
                     /locus_tag="PKX01_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ74985.1"
                     /translation="MTAPDFQLDLRLVRRRAGRAAATYAEVDALAREVARRMGERLDY
                     IRIAPSRILDLGCGPGADLPAFAARYPDVPRLAVDVARTMLALARDAHGPQQGFFQRL
                     LGSRGTPSAPGFLCADARALPLKRGAVSLVWSNLMLQWLHDPLPALKEMHRVLEVGGM
                     LMFSTLGPDTLRELRSVLPGPDGARLHRFIDMHDLGDALVQAGFSDPVMDMEMLTVTY
                     ASLDDLLRDLRLSGSACASAGRPRGLSGKGHWSGLRAAYEALRRNGRLPATVEVVYGH
                     AWKVAPKVAEDGRAIVRFERPPR"
     gene            complement(12517..13506)
                     /gene="bioB"
                     /locus_tag="PKX01_02325"
     CDS             complement(12517..13506)
                     /gene="bioB"
                     /locus_tag="PKX01_02325"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HMZ74986.1"
                     /translation="MTATVAPHEPLTTAPPSTPRKRWSVAEVDALFALPFNDLIFRAQ
                     QVHRAHFDANAVQRSTLLSIKTGGCSEDCGYCSQSARYDTGLEKETLLPLGEVLDNAR
                     AAKAKGATRFCMGAAWRGPKEKDLAPVLDMVREVKAMGLETCVTLGMLKDGQAEQLKD
                     AGLDYYNHNIDTAPEFYGQIITSHTLQDRLDTLDKVRKAGINVCCGGILGLGETRKSR
                     AAFIAELANMDPPPDSVPINNLVQIEGTPLGNAEAIDPFDFIRVVAAARITMPESYVR
                     LSAGRQELGDGFQALAFLAGANSIFYGERLLTTDNPDADADDRLFARLGLKSA"
     gene            13623..14279
                     /locus_tag="PKX01_02330"
     CDS             13623..14279
                     /locus_tag="PKX01_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HMZ74987.1"
                     /translation="MLPQACFACGAPSGDGVLCDACYGALPGRDCPRCPRCALPQPGG
                     GLCGACLRETPPFEATRVALDYAFPADSLVLALKYAHRLATTRLFVDLLAAAPRPEAD
                     LVVPMPLHPARLAQRGFNQAAELARPLARHWQLPVRLDGVVRDVNTVPQAGLPWKARA
                     ANVRGAFRVTGRLDGRRVVVVDDVMTTGATLAELARALKAAGAVSVENRVLARTPPPA
                     "
     gene            14313..14777
                     /locus_tag="PKX01_02335"
     CDS             14313..14777
                     /locus_tag="PKX01_02335"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HMZ74988.1"
                     /translation="MLHVVLHQPEIPPNTGNIMRMCANSGARLHLVKPLGFTLEEKQL
                     VRAGLDYRDLAVVTVHEDWPACRAALAGRRCFALTSHGGRHYGDVAFADEDVFLFGAE
                     SRGLPAELHAAFDDEHRLRIPMRPGNRSLNLSNAAAVVVYEAWRQLGFAGGI"
     gene            complement(14793..15773)
                     /locus_tag="PKX01_02340"
     CDS             complement(14793..15773)
                     /locus_tag="PKX01_02340"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMZ74989.1"
                     /translation="MHIGVFGAGAWGTALAIAFSRQHRVTLWSREAREIADLRSDGEN
                     RRYLPGIPLPAGLHPSDSLAEAARADLHLVVTPLAGLRATARALHDLAPGTPLLWACK
                     GLEAGTAQLPHQIVADELGGDAPCGVLTGPSFAAEVAKGLPAAVTLAARDLAFARHWV
                     NALHDPRLRLYANDDLVGAEVGGAVKNVMAIAAGVADGMGFGLNARAALITRGLAEIT
                     RLGIALGGRRETFMGLAGLGDLVLTCTGDLSRNRRVGLLLAEGKTLPDILATLGHVAE
                     GVSTAREVAALAARLGVDMPITAAVDGVLHGGLGAREAVEQLLARDPKQE"
     gene            complement(15773..16225)
                     /locus_tag="PKX01_02345"
     CDS             complement(15773..16225)
                     /locus_tag="PKX01_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ74990.1"
                     /translation="MPLRRLTVAALLALPCALAATPASASDFRSLAEPAILYDAPSKQ
                     GKPLFVIQRYTPVEMVVSVDRWVKVREPAGSLLWLERRQLSEKRTLIVTAARAEIRQK
                     PDGNAPLAFEAAKDLVLELADAPADGWVKVRHRDGSTGFIRMTQVWGL"
     gene            complement(16225..16683)
                     /gene="secB"
                     /locus_tag="PKX01_02350"
     CDS             complement(16225..16683)
                     /gene="secB"
                     /locus_tag="PKX01_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HMZ74991.1"
                     /translation="MSETAQQPVFSIEKLYVKDLSVEVPNAPQIFLEREAPQIGVQLR
                     TESTAVDDGVFEVNLTVTVTAKLPDDKTVFLVEVAQAGIFQIRNVPQEDLEPLMMIGC
                     ANILFPYAREAISDAVSRAGFQPVILAPVNFEALFQQSRQASAGTDIPVQ"
     gene            complement(16734..17003)
                     /gene="grxC"
                     /locus_tag="PKX01_02355"
     CDS             complement(16734..17003)
                     /gene="grxC"
                     /locus_tag="PKX01_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HMZ74992.1"
                     /translation="MQPHILMYATGVCPYCVRAEGLLRRKGVTEIEKVRIDLDPVRRD
                     EMMSRTGRRTVPQIYIGDFHVGGCDDLFELDHQGRLDPLLRGEGA"
     gene            complement(17010..17435)
                     /locus_tag="PKX01_02360"
     CDS             complement(17010..17435)
                     /locus_tag="PKX01_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ74993.1"
                     /translation="MEFLQQNWYWALLAAVSGSLLVATSLQRGGQGISPAQATQLMNR
                     EDALVIDVRDAGEFAAGHVPNARHIPLADLEKRLGELESFKDKPVIVNCQSGGRSVNA
                     CNVLTKAGFTRVHNLDGGIQAWEQANMPLGRSGKSKKKA"
     gene            complement(17479..17802)
                     /locus_tag="PKX01_02365"
     CDS             complement(17479..17802)
                     /locus_tag="PKX01_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ74994.1"
                     /translation="MTSPIVDLIAKQEHIETAARALKAISHPLRLKILCVIGAEEACV
                     QEIVEAVGTSQSNISQHLAILRDKGVLLTRKDANRVYYRVGDTRTLQLIVMMREVFCG
                     DSAKG"
     gene            18050..19474
                     /locus_tag="PKX01_02370"
     CDS             18050..19474
                     /locus_tag="PKX01_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ74995.1"
                     /translation="MRSKLQQAGLVFTGLCAGILISLNFSANADKNSLPPLPVEELRN
                     LADVFNAIKQGYVEPVDDKKLITYAISGMLSNLDPHSTYLDPEAFKELQVGTQGEFGG
                     LGIEVGSEDGYVKVISPIEDTPAFRAGIKAGDLIIKLDDTPVKGMTLNDAVKRMRGKP
                     KTSIVLTIIRKGEAKPLTVTLVREIIKVQSVKSKLVEPGYGYVRVVQFQEQTAASLVQ
                     HLDRLVKQGELKGLILDLRNDPGGLLNGAVGVSAAFLPPKALVVSTDGRTDDARRKYF
                     AVPDDYLRGSRDDYIRNVPASIKTVPMVVLVNGGSASASEIVAGALQDHKRAVVMGTQ
                     SFGKGSVQTILPLNSNAAIKLTTARYFTPSGRSIQAKGIVPDIVVEETPNGSRERLRE
                     ADLDRHLVNAGEPDAPKSETPASRKDPAGKPRDKDNRDNKDKPKDEADEVKPAFEIAG
                     KDDYQLAQAVNLLKGLQILQNSAK"
     gene            19497..19952
                     /locus_tag="PKX01_02375"
     CDS             19497..19952
                     /locus_tag="PKX01_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ74996.1"
                     /translation="MRRETAYKLAGRKHDHASHHAGSGLTKSREIRFKDLPPGQAVQA
                     RRSLAALKGVRVTDGKHPLELVVHYNVLDYTLELLEVALQNAGFHLDRPLLVRLHRAL
                     IYYVEETQVHNLRSPERLIKQSHEVYVKAYAAHPHGDHDDTPLELREYK"
     gene            20011..20760
                     /gene="moeB"
                     /locus_tag="PKX01_02380"
     CDS             20011..20760
                     /gene="moeB"
                     /locus_tag="PKX01_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HMZ74997.1"
                     /translation="MNDDQLLRYSRHILLPQIGIEGQSAIVSARVLVVGAGGLGSPAA
                     MYLAAAGVGTLVLADGDTVDLTNLQRQILHGEASVGRLKVDSGQETLSRLNPETRVES
                     LPRRLEGRELDEQVALADVVLDCSDNFATRHAINRACVAHRTPLVSGAAVRFDGQLSV
                     FDLRTDASPCYHCLFPEGEDVEEVRCAVMGVFAPLTGIVGTVQAAEALKLLAGCGETL
                     TGRLLLLDALEMDWRSVRLTRDPTCAVCASR"
     gene            complement(20788..21753)
                     /locus_tag="PKX01_02385"
     CDS             complement(20788..21753)
                     /locus_tag="PKX01_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ74998.1"
                     /translation="MIQSLTDAFIALQAWLFDALVQPVMFALGLGSFVELGFDGVEFV
                     LLGLLELAVAYLLLRPLEALWPAERWAARRAVRVDMLYTFLNRLGIVPLAVFLLLAPL
                     FAEVESWLRFHDLLPRQLEDLLPALDHHPLVSFLLYLVILDFAEYWRHRLSHVFRWWW
                     ALHGIHHSQRQMSFWTDSRNHLADDLIAGLWFAGLALFIGVPPGHFIGLLIALRLVEN
                     LSHANTRFDFGPIGDYLVVGPLYHRWHHALDLPAGPRYRHGCNFAILLPVWDVLFGTL
                     YLERRWPATGLHEASPEESNARDHFWAQQKLGFKLLWKALIGKPA"
     gene            complement(21750..22487)
                     /locus_tag="PKX01_02390"
     CDS             complement(21750..22487)
                     /locus_tag="PKX01_02390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ74999.1"
                     /translation="MKHLAALALTALVPAVAGAGKADCPAGTLYLTLDTGNMRHAEAI
                     ADILARHHIRATFFVAQEKTLRGDHALDASWAPFWKARVAEGHAFGSHTWRHGSFRKD
                     GADGRVSYRLMDGSTVQLDSAQVCEELRQPDRRFAELTGRPLDPLWRAPGGRTTPLTL
                     QAAEACGYRHVGWAPAGFLGDELPSDAFPNEVLVRRALDTLKDGDILMAHLGIWSRKD
                     PFAPMLDPLIGELQRKGFCFATIPERP"
     gene            complement(22484..23443)
                     /locus_tag="PKX01_02395"
     CDS             complement(22484..23443)
                     /locus_tag="PKX01_02395"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ75000.1"
                     /translation="MRLPRFLLALTASVLPALACAETALVLNSADETVSFVDTVAYKE
                     TGRAPACKEPHHLMSTPDDSSVIVACAISNELVFFDPRNGKEQKRLHGISDPYQLGFS
                     PDNKWFVANSLRLDRVDLYTAADFKLVARVPAAKTPSHMAFDKASQFVFITLQDSNEV
                     LAIDLKTQQPLWKLPVGPTPAGIHMTPDDKYLLVGIMGADYVEVIDWRARKTVKRIVT
                     DKAAHNFLARGDGRHVFVSNRTVRGSISLVDMQTLSVVEKYDVPGGPDDMEIRADGKE
                     LWATARFARKVQVVDLEQKKLARSIPVGRSPHGVFFLNHAPRQ"
     gene            complement(23565..24653)
                     /gene="ribD"
                     /locus_tag="PKX01_02400"
     CDS             complement(23565..24653)
                     /gene="ribD"
                     /locus_tag="PKX01_02400"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMZ75001.1"
                     /translation="MSASVDDTRWMARALQLAELGLFTTTPNPRVGCVLVRDGQVVGE
                     GWHRRAGEPHAEVHALAQAGDRARGATAYVTLEPCAHHGRTPPCADALVRAGVARVVA
                     AMEDPNPLVAGRGLARLREAGIATHCGVLETEARELNIGFVSRMTRGRPWLRLKAAAT
                     LDGKTALHNGVSQWITGPDARRDAHRWRARSCAVLTGIGTVRDDDPQLNVRAIPTERQ
                     PLRIVVDARLETPLHAHVLDGGPVLIAGAVDDPARIAALQARGADVLILPNDAGKVAL
                     PALLAELGRRGLNEITAESGFKLNGSLLREGCVDELVLYLAPTLVGDAARGLFNLPAL
                     DALADQRRLNFRDVRQIGPDLRIVARPA"
     gene            complement(24656..25135)
                     /gene="nrdR"
                     /locus_tag="PKX01_02405"
     CDS             complement(24656..25135)
                     /gene="nrdR"
                     /locus_tag="PKX01_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HMZ75002.1"
                     /translation="MRCPFCSAENTQVTDTRENEDGDIVRRRRRCLTCDKRFTTYERV
                     DLKMPQVVKRNGSRADFSRDKLLGSLSLALRKRPVMAESVEAAVDRIEAKLLSLGERE
                     VASELIGELVMRELKKLDKVAYIRFASVYRAFEDVDEFSEVIREVSPRPRGRPRSTS"
     gene            complement(25159..26409)
                     /gene="glyA"
                     /locus_tag="PKX01_02410"
     CDS             complement(25159..26409)
                     /gene="glyA"
                     /locus_tag="PKX01_02410"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMZ75003.1"
                     /translation="MFSKQNTLARVDPELWQTVEAENRRQEDHIELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEHVDVAEQLAIDRLKALFGADAANVQPNSGSQAN
                     QAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNLSGKWFNVVAYGLDDKEEIDYEAMEK
                     LAREAKPRIIVAGASAYALRIDWERFAKIAKEVGAIFWVDMAHYAGLIAAGFYPNPVP
                     HADVVTSTTHKTLRGPRGGVILMKAEHEKAINSAIFPGLQGGPLMHVIAAKAVAFKEA
                     ATPEFKNYQEQVIANARVMARVLGEERGLRIVSGRTESHVFLVDLRAKNITGKEAEAA
                     LGRAHITVNKNAIPKDPQKPFVTSGIRIGSPAMTTRGFTEIEAERVAHLIADVLDNPN
                     DEATLAAVRSKVAELCRKFPVYGA"
     gene            complement(26486..28519)
                     /locus_tag="PKX01_02415"
     CDS             complement(26486..28519)
                     /locus_tag="PKX01_02415"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75004.1"
                     /translation="MSRLNRLSLVHQIALAAGLISLIVFGTLVTFTTYFAEKADLAKT
                     GDELGHQIQGVVRMLELNHAGAVARAGSSLGRVQQAFGPLRIGPDTMASGSYQVPVVR
                     SGERILNGNKALLETLRDQIDADPAVLLRVGDEFVRAATLLRDKDGKSTEGTPLPRDG
                     KETRALLEGKSYRGVVRRNGTFFISAIDPMRDADGKVIGALAARVDIQSDMARLFAVL
                     GETRSGATGYTYVIAPGADAATSEVVFHPTLSGKKLNELNNPVLSRVVGEQIRLGKGT
                     LTYDWPANGGGEKTKMVAFDTAPSWGWIVATGSFVDEYTVHARELLRVLILLCTAGAA
                     LLVAVLYLIARSRIAPIRELVAVTERIGRGELNVAFPAVPDPTRNEVELITRALDSTT
                     RQMAGLIGGVTHTANAIKGAAHSLRADSESIVTSTARQSESASGLAAAVEELSVSITH
                     VSDSAALAREMTQDASAHADDGQHRMREMIDGMGRIAADMDTASAAVNSLSQRSARIS
                     NIGRIINDIADQTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTASSTREISET
                     VTLVQDEAQRVVDTIQGITTGVQNGVAVATASGAMLETIRSESARTTGAVNDIADATL
                     EQSSASQEVARNVEQIARMAEQNNQIIRDSHAQTTQLEQLADELEARVSHFRL"
     gene            complement(28610..29248)
                     /locus_tag="PKX01_02420"
     CDS             complement(28610..29248)
                     /locus_tag="PKX01_02420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ75005.1"
                     /translation="MITRICLVRHGETAWNAERRLQGHTDIPLNDTGLAQARATAASL
                     AAEGFDAAYSSDLLRARQTAEAIAQRCALAITHDASLRERHYGHFQALTYDEARARFP
                     DDYHRFEGRHPDFVFPGGGESLNGFAARIRAALERIAATHPGRKVLVVTHGGVLDVAH
                     RLATGKPLDTPRDFTIPNAALNWLGWDEGRWSLIEWARQSHLHGALDELPNS"
     gene            29424..30014
                     /locus_tag="PKX01_02425"
     CDS             29424..30014
                     /locus_tag="PKX01_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HMZ75006.1"
                     /translation="MQALLKLSRGIDALTDRVGRTTTWLVLVVTLISAINAFVRYAVN
                     YSSNGLLEIQWYLFSAIFLLCAGYTLMRNEHVRIDVVTGHLSPRAHAWIDIVGTLFFL
                     APMALLVLYLSWPIFVNSFLTHEQSSNAGGLTVWPVRLLVPVGFALLILQAASEFIKR
                     VAFLKGLIDDPSAKHKGPSAEEGLAEAIRRQRGEEA"
     gene            30015..31565
                     /locus_tag="PKX01_02430"
     CDS             30015..31565
                     /locus_tag="PKX01_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HMZ75007.1"
                     /translation="MEFLAANMAPLMFGSLVIFLLFGYPVAFALAANGIVFGLIGIEL
                     GLLSPALFQALPDRIFGVMSNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPVR
                     GGLAFAVIFVGAILAATTGVVAASVISMGLISLPIMMRYGYDQRLATGVIAASGTLAQ
                     IIPPSLVLIIMADQLGRSVGDMYQGAMIPGLVLTGFYVAYVALVAVFRPAAAPALPPE
                     ARSLRGMTLAIRVLTTLVPPLMLIFLVLGTIFLGVATPTEGGAMGAFGALVMAVLRRR
                     LNLSLLRQAMETTAKLTTFVVFILVGARVFSLTFYGVEGHLWVEHLLSGLPGGRLGFL
                     IVVNILVFLLAFFLDFFELSFIVVPLLAPVAEKLGIDLIWFGVLLGVNMQTSFMHPPF
                     GFALFYLRSVAPPSIKTTQIYWGAVPFVLIQIIMVGVIITFPDLVTMGLDSQEKVDAD
                     AIEIVVPQGDAPDDAPVQFDAGKDGDAPHAGDGQAGAGEQKDGIPVPPADDDATRALE
                     KALREDRR"
     gene            32066..34144
                     /locus_tag="PKX01_02435"
     CDS             32066..34144
                     /locus_tag="PKX01_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75008.1"
                     /translation="MALLTSLSPDVFLERLRAALAEPRRDHPELARLARRLAAFIAAE
                     DLPDRLDAWHALMTWLAGGRLWQDVYIDVVDVHGPATQRLAVLMDVLAGDAELAGALR
                     AALRGLLDETDGANLFGEVGIPSDRGFLSEFGDRLTAKLLPQPRVDHDLAHLVGRSYR
                     RRRDLDLVRALHPSTLQRLMELLFPADGPEMTRALRRAMADGFRLLLARVAGQGLSEK
                     LRARGSAQAVADVPFARLARFGEALVADWEGGALPDAAMDAWQALSHACRAEMQVVSR
                     RLESEGISVDIVYGLDVLERCLNRLDALVAVMVEGGAAPVLALVQQLVLAAHQSSSLR
                     HLVRINLQRLQRRIVDRAGQTGEHYIADTPGEYRHIWTAAAGGGLLTVGTAAMKMTIA
                     GAGLALFLEGLGSGLNYAVSFLLLQAFGLILATKQPAMTAAAFAAIIRDRQGAERLDS
                     IVDAAARICHSQLAATIANVVVVAFGAYAFNGLWAWITGHAFLTASEARYVFKALSPV
                     DSGTVFYAALTGVILYLAALVGGWVDNWAVYHRLPEAIAEHRLGQRVGRLRMLRLAGI
                     VSRNMAGWATNVSLGLMLGMTPVLGRFFGLPLDVRHVTLNSGILSLAAAGMDLQWIGT
                     GHLSLAIAGVAVMFVLNLGVSFALSLLTAIRAYELPAEDLRELRRRLWQRLRTRPQDF
                     LMPRRLPPAP"
     gene            34277..34903
                     /locus_tag="PKX01_02440"
     CDS             34277..34903
                     /locus_tag="PKX01_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HMZ75009.1"
                     /translation="MNAPERVPFAGSRAVAGELACIVRVNEEIKAVVATATRINLMAL
                     NAIFLAKRAGNAALGFGVLSNELRRFAQDLTRHMAVLRDMTSGSVGSVTGVVQHQRNS
                     RILSEAIRLSGEGVPGVDMARRRCNQRLARELASLRGLDQRLRAAVEETQQLVELGGV
                     LARSAKIEAAYGGGFSPALMQVSTDFADVIAQIKRSLENLSKERLVKD"
     gene            34909..35649
                     /locus_tag="PKX01_02445"
     CDS             34909..35649
                     /locus_tag="PKX01_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ75010.1"
                     /translation="MKQAHYIYQDGDHRWLAIARDPNKPGYVIDTNEYVVADEEAALL
                     LDPGGIEIFPAVFSALSGECDPTRLMGIFASHQDPDIISSLALWLEFKPDLKCYTSWL
                     WSSFLPHFGGASETFVSIPDGGMTIKVGSLDLQAVPAHYLHSSGNFHLYDSKAKILFS
                     GDVGAALLPPDNNGLYVEDFSRHIRHAEAFHKRWMGSDRAKRDWCERVSRLEIDMLVP
                     QHGAIYQGPDVQRFINWFAALEVGVLRP"
     gene            35811..36200
                     /locus_tag="PKX01_02450"
     CDS             35811..36200
                     /locus_tag="PKX01_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HMZ75011.1"
                     /translation="MYLAIKHLHITCVALSLAGFLLRGLWMCADSPLLYASLTRRLPH
                     IVDALLLGSAIALAVMSGQAPFVQAWLTAKVAGLLVYIVLGALALRPGRPKPVRLGAF
                     GAALVSFSYIVSVALTKNPAGWLAMWG"
     gene            complement(36204..36944)
                     /gene="fnr"
                     /locus_tag="PKX01_02455"
     CDS             complement(36204..36944)
                     /gene="fnr"
                     /locus_tag="PKX01_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HMZ75012.1"
                     /translation="MHTKPVIPLSVTEVKIACSQCNLRELCLPFGLDPNEIDRLDELV
                     GSRRKIKRHQHLYRSGDPFEAIYAIRTGSFKTDVILEDGRDQVTGFQMTGEVMGLDGI
                     SIEHHTCNAIALEDSEVCVIPFGRLEELSREVESLQHQFHKIMSREIVRDHGVMMLLG
                     SMRAEERLAAFLLNMSQRFTARGFSPSEFHLRMTRDEIGSYLGLKLETVSRAFSRFQD
                     EGFVSVQQKHIRILDIAGLKALLNHGPT"
     gene            37067..38461
                     /gene="hemN"
                     /locus_tag="PKX01_02460"
     CDS             37067..38461
                     /gene="hemN"
                     /locus_tag="PKX01_02460"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HMZ75013.1"
                     /translation="MNTIHKDLIFDPQLIRKFDVNGPRYTSYPTADRFVEAFNSEALC
                     DWLGKRTIGGVGKPLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLRYVAREMEMQ
                     VAHLTGPREVLQLHLGGGTPTFLSHDEMRELMANVRKHFTLVPDGEFSIEVDPRKVDE
                     ATVALLAELGFNRMSVGVQDFNPDVQRAVNRVQSEEETRLVIESARAHGFKSVSVDLI
                     YGLPKQNVISFNRTLEQILALSPDRLSIYNYAHLPGLFKPQRRIHDAELPNAEAKLQI
                     LQLAIRRLTEAGYVYIGMDHFAKPDDELAVAQRQGRLHRNFQGYSTYAECDLLAFGVS
                     AIGKVGPTYAQNVKTLDEYYDIIDAGRLPVLRGIELTPDDLLRRSIIQALMCHFELSI
                     ESIEIAHLIDFPRYFAAELADLREMEKNGLVQIDDKWISVQPAGRMLVRAIAMVFDRY
                     LRADRERTRYSKVI"
     gene            38610..39317
                     /locus_tag="PKX01_02465"
     CDS             38610..39317
                     /locus_tag="PKX01_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ75014.1"
                     /translation="MPETGYLAVFLIGLLGGTHCVGMCGGIVSALTVRMPGEQRRDWP
                     LHVAYNFGRIASYTAAGAALGALGSAGMLLDGMLPVQLGLYVAANLMLVALGLYLTGF
                     TRALAGVERIGQRLWTRIQPLTRRFLPARSVAQALPLGMLWGFLPCGMVYSVLATALV
                     TGSAGRGALLMLSFGLGTLPNLLLAGLLLRHLREVVRNRSVRLVSGLVVLGFGVYGLL
                     NTSSLGNKLWNGVVCHV"
     gene            39348..39773
                     /locus_tag="PKX01_02470"
     CDS             39348..39773
                     /locus_tag="PKX01_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ75015.1"
                     /translation="MKILLAVDGSPVSLRAAEEVVRLARALKSEPDVHVVYVHLPIPL
                     DFATRHVGREAVEAYYRDEGEAVLQAPLAALTASDLMPQRHLHVGQPADVILQLAGEL
                     GCDLICLGRHGRGAMADLLLGSVANRVVQRAPMPVLLVG"
     gene            39783..42182
                     /locus_tag="PKX01_02475"
     CDS             39783..42182
                     /locus_tag="PKX01_02475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ75016.1"
                     /translation="MSDGPLPESMDELSITGMSCAACAVRLEKVLSRLPGVSAHVNFA
                     TGRASVRRATGAADGEALVAAVRKAGFDVAPETLELALSGMSCAACAARIEKVLNRLP
                     GVEATVNFNAETARVRYVPGLAGPDAMRAAVAKAGFSAEPVLARDREAERARKAEEAR
                     SEWRQFLLALVLSAPFLLQMPFMLAGGLDGHHEWLPRWLQGLLAAPVQFWVGRRFYRG
                     AWSSLRGGGANMDVLVALGTSVAFFFSLAVVLLGRHDQHVYFEASASIITLVLLGKLL
                     EARARARTSAAIEALLRLQPPTAWLERDGALVEVPVAQVARGTVFVLRAGDAVPVDGE
                     VLAGESSIDEAMLTGESVPVPKAAGDTVFAATLNGAGLLRCRATGVGAETLLAGIVRL
                     VEQAQGGKPAVQRLADRIAAVFVPTVVGLALLTFAAWWWAAGDPVQALVNGVAVLVIA
                     CPCALGLATPTAIMVGTGQGARAGILVRKVEALELAESLRIVAVDKTGTLTEGRPALV
                     ETRLAKGIAYNEALHLAASLEQGSPHPLAVALVAAAREAGIGLALPVAVQSAGGKGVS
                     GEVAGRQVAVGSPAYLTEAGVAIPADQLDPLTTGGLTPVCVAADGRLLALFGVADRLR
                     PGAAEAVARLRRQGLRVVMLTGDHPAVAQAIARQAGIDEFRAQVLPADKAAAVQALRN
                     EGRVGMVGDGINDAPALAAADVSFAIGAGSDVAVEAADVTLVRSDPAGVADAIALSRA
                     TLGKIRQNLFFAFIYNILGIPAAALGLLNPVIAAAAMAMSSVSVVSNSLLLRRWRPGQ
                     D"
     gene            42201..42407
                     /locus_tag="PKX01_02480"
     CDS             42201..42407
                     /locus_tag="PKX01_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMZ75017.1"
                     /translation="MQEAIIQVGGMSCQGCVRNVTGALSAVAGVTAVEVSLERGEARV
                     NFDPARTGVAALRAAVEEAGFDAA"
     gene            42491..43018
                     /gene="ppa"
                     /locus_tag="PKX01_02485"
     CDS             42491..43018
                     /gene="ppa"
                     /locus_tag="PKX01_02485"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HMZ75018.1"
                     /translation="MGYDQVKAGKDVPNDINVIIEISAQGDPIKFEVDKDSGAVFVDR
                     FMGTTMRYPINYGYVPHTVAGDGDPVDVLVVTPFPLQPGVVIRARPVGVLKMEDEGGV
                     DAKVVAVPVSKLTPLYDKVQSVEDLPELLMKQTVHFFEHYKDLEPGKWVKVTGWGTVE
                     DARQEILNGIAAAAK"
     gene            43290..43706
                     /locus_tag="PKX01_02490"
     CDS             43290..43706
                     /locus_tag="PKX01_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ75019.1"
                     /translation="MTKQKQSGFTLIELMIVVAIIGILAAIAVPQYQSYTKKAKFSEV
                     VNATSSRKSFVEVCLQDPNLVQANCNGGANGIPADDAVGFGNVTSISVASGAITAIGK
                     SGVFGSGATYSLNPTVTSGNVVWSDTSSACKAAGLC"
     gene            43796..>47435
                     /locus_tag="PKX01_02495"
     CDS             43796..>47435
                     /locus_tag="PKX01_02495"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HMZ75020.1"
                     /translation="MMTDSDVRRKFSSPGMVSFLRHTQLAGPFVVWILPACFALASLA
                     IVFGQIDDLLSPFAVWSTPGSRAMAYFIVGTLVLPVYAFTRPPVKELALVLGGGLFSG
                     WFIGVSAEQLFSSGVTGFIAPFGFPFFCMAVVALVGLTARALLTRNTIAVMSDPRVSA
                     LMACMFALLYMFFSPTFVNVTIIFHPETFDNRLYALDALFGFEASVFVGKLFVTHSAV
                     LTFFQLAYAFMFVSFLAVLGSQIREEWRGQGWQVAEVWFVISILGVMAYHLFPVSGPV
                     YAFKDKFPASMPDPSGVGLVSAIVAPAMRNGVPSLHLGWSLAAWLVSLPLRNRTIKWV
                     SGLVALAMVPATLGLGEHYLLDLVIAVPFVVGTLALTVRGPGWQSPARRMAAWTGWGF
                     FFVWLILLRFAAEWLLQAPWVVPVLSIVSSGASAWRMRALICAVRGGGEVAEVTRVDQ
                     ASALGGSFSVRLAALFFLSGFAALMYEVLFSKKLAVTFGSSSLATYTVLATYMGGMAI
                     GSWLGGILAARGGDLLKRYVVCEGSIAAYCVLTPFLFDAVHDLYAQWATNAPVDAGWV
                     VALRFGLGALVLMVPTILMGMTLPILTACLDRGAAAYGRVVSVLYGANTLGAALGALL
                     AGYVLIPTLGLQSTTLLAALLNLLVAAFGGEMGKRLKQVGARLEQDEDATSALSASGR
                     ALPGWLVLMSFGGVGLVCLGLETLYMHLLAVVAGNSTYAFSLMLFAFLLGLGGGATVI
                     RQIPQAISRPEVAMVVLLMLLAMCLGIGESQWSTMADYFASFAGYPMPSSFGGRELVR
                     AAVCLIALVPPAFLIGAFFPLAMGAVGRAGGVRALGTGAAVNTLGNIAGVFIGGFVLL
                     PTVGALGGIKVLAVLAMGLAVCIVIGAGLMRERVLVALGGMAALCVVAAPTDLDYTRL
                     ASGANVYFQPQHWGRVIDHAESADGGLTSVHVTLQDGGMTMKTLTTNGKFQGNDSGEV
                     EAQIGFALVPLAENPRRNDALVIGYGTGTTANTLKHAGFRQIDVVDLSRDVIRLANAH
                     FAHLNERVTEAPGVKLHIADGRNYLLLQHRQYDLITVELTSIWFAGAAASYNREFYQL
                     ARQRLRPGGVLQQWVQLHHLSGQDLLALLASVRSEFADVKVFHFGGQGMIVARNGGGA
                     PMAVGMGRPQDEPAIRAYSERILGRPLAKGPERLLDAQGVNNFLSHWGEAEDWASTDD
                     NMVLEYNTP"
     assembly_gap    47436..47535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47586..48182
                     /gene="ampD"
                     /locus_tag="PKX01_02500"
     CDS             47586..48182
                     /gene="ampD"
                     /locus_tag="PKX01_02500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HMZ75021.1"
                     /translation="MISPNCDTRPADCPVVLIVLHAISLPPDRFAGDGVERLFTNRLD
                     SAGHPYYAAIHHLRVSAHYFIRRTGEQICFVAPALRAWHAGVSSWRGRERCNDFSIGI
                     ELEGCDSLPFEPLQYEVLADLLATLCEQFPIEGITGHADIAPGRKTDPGPYFSWERLR
                     RLLTVRGGAALAARVEPESALDESVQMRWPFPSNRRPV"
     gene            48669..49367
                     /locus_tag="PKX01_02505"
     CDS             48669..49367
                     /locus_tag="PKX01_02505"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ75022.1"
                     /translation="MFATRIVKYARHAASFTLHFTHGGLLVAGLIVTLFVASRIATHG
                     VQGLALGSLLGSSPVVAAQAPDSEVQDQTEEVGQEIALSPDLQRVKSYLAKRYQVSAV
                     AIEPLLASAQQTGRSVGVDPLLLVAVMAIESRFNPFAESPVGAQGLMQVIPRFHTDKV
                     DVKSDKAALFDPDTNIRVGALVLKEYIRNTGSVERALEQYGGSSDPATPYAIKVMAVK
                     AQLAAAARGKIIGA"
BASE COUNT         7620 a        14622 c        18100 g         8935 t
ORIGIN      
        1 cgcgggcgag cacgatgcag ttggtgacga tgagcgggat gaagatgccg agcacgaggt
       61 agaggtcgtg cacgaaggcg ttcatggaga gatccaccac ggtggtgagg gcggcgatga
      121 tcaggatgaa caccgggatg cggatctcgt aggggatgaa gttgcgcagc gccgccacgg
      181 cgaggttgga catgcccatc acgaacatgg tggcgagccc gaggctgagg gcgttgacga
      241 tgttggtggt gatggcgagg gtggggcaca tgcccaggat ctgcacgatg ccggggttct
      301 gcttccacca gccgttccag gcgatgtcgc gataggcctt gatgtccatg gtcttcctcc
      361 gctcagaggg gcttgccgct gggggcggca aagaggcggt tctggttgtc ggcggcaaag
      421 cgcgcggcgc gggccaccgc gttggtgacg gcgcgggcgc tgatggtggc gccggtgtgg
      481 taggcgatct ggccaccgtc cttgcgcacc ttccattggt ccagcggggt gctggcgggg
      541 ttcaggtcgg tgaactggac gatccacggg gcggctttgt tcttgtcctt cttgggatcg
      601 atgtagtcgc ccaggccggg ggtttcccgg tggccggtga cgcgcacgcc agagaggctg
      661 ccgtcggcgc gcacggcgac gatgaggttg accttgccgc tgtagccgtc cggggcgatg
      721 gcctccagca ccagcgcggc gggctggccg gccttgcggg cgcggtagat gcggccgccg
      781 tcgtccaggc ccagggccgg cgtggggccc gtcaggatga agtcgtcgag gagggcgttg
      841 tcgtaggcgg caggggggag cacctcggcg atggacttca tcttctcgtc gttggccgag
      901 gcggcgatgc gctcgcgggt ggcgtcttcg gtgaaggcca tgagggcggt gaataccacc
      961 gtgaaggcga tcatcacggc gccggtgcgg gtggacaggc cgagggcggt ggtttcgctc
     1021 atggcgctca gtccttcttg tggccgaaga ccggggactg ggtcttcatg tcgatgaggg
     1081 ggacgaggat gttcatcagc agggtggcaa aggcgatgcc gtcggggtag gcgccgaagg
     1141 cccggatgat gtaggtgagg caggcggccc cggcggcgaa gaggagcttg ccgcggggcg
     1201 tggtggcgcc ggagacgggg tcggtgagga tgaagaaggc gcccagcatg ctgccgcccg
     1261 aggccaggtg gaagaggggc gaggcgaagc cggcgggctt gacggcccac agcaggccgg
     1321 ccacggcgaa gagggccgcg cagaaggcga agggaatgtg ccaggtgatg atgcgccgct
     1381 ggatcagcca caggccgccg agcaggtagc cgagggcgac ccattcgctg ccctggccgc
     1441 cgagggtgcc aaaggcgggg ccgtccatga gctgcgacgc cggcaggttg tagaggggcg
     1501 aatcgggatt ggcggcagac ttcagcgcgg tgcgcaaggc gtcgaggggg gtggcgccgg
     1561 tgatggcgtc cgcggcccgg gcgccgccga agatcaggtc gagctgggtg gtgaaatcga
     1621 gctggccggg ggcgggccac tgggacatca gcgccgggta ggcgacgatc atcaggcaga
     1681 aggcggccat ggccgggttg aaggggttct gaccgaggcc gccgtagagc tgcttgacca
     1741 ccacgatggc gagcagggtg gcgagtacgg tgatccacca gggcacgatg ggcgggaagg
     1801 tgagggcgat cagccaggcg gtgacgacgg ccgacaggtc ggtcaggaac agcgcccgcg
     1861 gccgcccgcg gacggtgagc agggcggctt cgccggccag ggcggcggcg gtggcgaggg
     1921 cgaggttgac gaggatgccg gcgccgaaga accagacgta ggcggcgatg cccggcacga
     1981 gggcgagcag cacctggagc atgacgctct ggacgctggc gggcttgcgg atgaaggggg
     2041 agttgagcat cgggttcgga aggcgggagg tccgtcaggc gctgccggcg ggcggttcgt
     2101 cggccttggc ggcggcgggc gcgtcggaag ccgcctggcg gcgggcgtcg atggcgtcga
     2161 tgtcggcctg ctggccgggc gagaggtgat cggtgttctt gggtgtgacc tgggccttct
     2221 gctggcgggc cttttccatg gcggcctcga tgagggcgcg cttggcgtcg tcaggactgg
     2281 gcttggcggc gggcgcggtg ccggttgccg gggcgtcgcc cgccggtttg gcggcggcct
     2341 gttcgcgggt ggcggcggcc ttggcggcga gcttttcggc tttttcctgc ttttcccgtt
     2401 cctggcggag gtttttgtat tcgaagcggt cgcgggcccg gtcggaggcg tccttgtccc
     2461 gctcccgtgc ccagatttcg ctcttggcaa agcggtagta gtccaccagc gggatctgcg
     2521 acgggcagac gtagctgcag caaccgcatt cgatgcagtc gaagaggtgg tattcctggg
     2581 ccttgccgaa gttcttggag cgggagaacc agtacagctc gaagggcgcg agatccgcgg
     2641 ggcaggcctt ggaacattcg ccgcagcgga tgcacggcat ctcgggcggc cgcggcggga
     2701 acagggcggg gctgccgacg aggatgcagt tggtggcctt gaccacgggc acgtcgaagg
     2761 acggcatgcg caggcccatc atggggccac ccatgatgta gcggtcggtg tcggcgccgg
     2821 ggccggcgag gcgcaccacg tggtccatag gggtgcccag gagcacgtcg tagttgcgcg
     2881 ggtgggccac attgccggtg acggtgacga cgcgcgacac cagcgggcga gcccggtcaa
     2941 gggcttcgtg gatggcgaag gcggtgccca cgttgaagca ctgcacgccg tagtcggtgg
     3001 agcggcggcc gtggggcacc tcgatgccgg tgaggacgcg gatgagctgc ttggcgccgc
     3061 cggccgggta gcgggtgggg atggccacgg cctcgatgcc ggcctcgccg cgggcggcaa
     3121 tggcggcctg cacggcggcc acggcttcgg gcttgttgtc ctcgatgccg atcaggactt
     3181 cttcggcctg gagcatggcg cgcatggcgg agatgccctt gagcatgtcg ccggcgcgct
     3241 cacgcatgag caggtcgtca caggtgatga agggctcgca ctcggcgccg ttgatgacca
     3301 gggtcttggt gtggccgact ttgccgggct tgagcttgag atggctgggg aagactgcgc
     3361 cgccgaggcc gaccacgccg gcgtcgcgca ggtagtcgcg cagctcgtcc ggagcggcag
     3421 acttgaggtt gatcgtctct agctcgcccc agcggtcctc gccatccggt tcgaggatca
     3481 ccgacagggc gctgaggccg gaggtgtggg gcatcagggc cggggccacg tcgcgcacga
     3541 ggcccgaggt ggaggcatgc accgcggagg acacgttgcc atccgcagca ccgatgcgct
     3601 gccccttgaa gacgcgttcg ccgggcttga ccagtgggcg cggctggccg cccacggcct
     3661 ggtgcagcgg gatcaccagc cggtcgggca tgggcaccgg ggcgatcggc gcggtggtgg
     3721 attgggcctt gtgggtctgg ggcttgacgc cgccgtggaa gctgaagagt ttgcgcagca
     3781 tgtcaggctt tgttccgggc cgtccgaagg gggctgaata ggtgtctcat caggccgccc
     3841 ggtcggcggg ggcggcttcg gcgaggggaa tcaccgggta gcgccacttc caggtgtcca
     3901 cggtctcggc gatggggcgc atgtggatgc aatccaccgg gcagggggcg atgcacagtt
     3961 cgcagccggt gcattcgcgg gcgaccacgg tgtgcagctg cttggccgcg ccgacgatgg
     4021 catccaccgg gcaggcctgg atgcacaggg tgcagccgat gcaggtgtgc tcgtcgatga
     4081 cggcgacgga cttgggtttt tcgacgccat gctcttcgga gaggggcttg aactcgcggc
     4141 cgaggagatc ggacagcttg cggatgcctt cttcgccgcc cggtgggcac tggttgatct
     4201 cggcctcgcc gttggcgatg gcggtggcgt agggccggca gccggggaag ccgcactggc
     4261 cacattgggt ctggggcagg atcgcgtcga tctggtccac cagcgggtcc ccttcgacct
     4321 tgaacttgat cgaggcgaac cccagcatcg cgcccaggac cagggcgatg gcggtcatga
     4381 cgaggagggc ggagagcatg gcggtgcggg tcagtggtac ttgtcgaggc cggcgaagcc
     4441 catgaaggcg aggctcatga ggccggccgt gacgaaggcg atggcggtgc ccctgaaggg
     4501 cgcaggcacg tcggcgccat cgaggcgttc gcggatgccg gcgaacagga tcagggcgag
     4561 ggagaagccc accgagctgc cgaaaccgaa gagcagtgac tggagcaggt cgtggtgcag
     4621 ggccacgttg agcaggggca cgcccagcac ggcgcagttg gtggtgatga ggggcaggta
     4681 gatgcccagc acctggtgca gcaccgggct ggtcttctgg atgatgagtt cggtgagctg
     4741 gacgatggcg gcgatgatga cgatgaaggc cagcgtctgc aggtagctca ggccgaaggc
     4801 gctgagcacg tagtgatcga tgaggtagct ggtgccgcag ccgagggtga gcacgaaggt
     4861 ggtggcagcg cccatgccga cggcggtgtc gagcttcttg gagacgccca tgaacggaca
     4921 caggccgaga atgcgcacca ggacgacgtt gttcacgaga acggcgccga ggatcacgag
     4981 aaggtagctg gacatgggcc gggcttcagc gtaggggcaa aggcggatta tcccgcgacg
     5041 cgggcgaaag actcaagtcg ttgaatcaag acggacatcc aaaccggatt caggccgcgt
     5101 cacgtgtggc tgcgacgcac tcgcgcacga gggcggggcc gcggtagatg agcccgctat
     5161 acacctggac gagctgtgcg ccggcctgca gcttggcaag cgcctgggcg ccgtcgaaga
     5221 tgccaccggc ggcgatgatg ggcagctcgc cctgcagggc ccgggcgagg gccgcgacga
     5281 cggcggtgga ggcttcgaac aggggtgcgc cggagaggcc gccctgctgg tcgccgtagg
     5341 gcacgccctg gaccttgtcg cgggcgaggg tggtgttggt ggcgatcacc gcgtcgatgc
     5401 ggtggcggcg caaggcgtcg gcaatgttga ggatctgggc gtcgtccaga tccggcgcga
     5461 tcttgagggc gatgggcacg tagcgcccgt ggcggtcggc gagggccgtc tggcgtgcct
     5521 tgagctggcc gaggagggcg tcgagttcgg attcgccctg gagctggcgc aggttcttgg
     5581 tgttgggcga ggagatgttg acggtgacgt aactggccag cgggtaggcc ttgtcgaggc
     5641 aggcgaggta gtcgtcggcg gcccgttcga tgggcgtgtc ggcgttcttg ccgatgttga
     5701 tgccgaggat gccccggtac ctggccgcct ggacgttggc caccagggcg tcgatgccgt
     5761 ggttgttgaa gcccatgcgg ttgatgatgc cgcgcacctc gggcaggcgg aacatgcgcg
     5821 gcttgggatt gccggcctgg ggccgcgggg tgatggtgcc gatctcgatg aagccaaagc
     5881 cgaagcgggc cagtccgtcg atgacctcgc cgttcttgtc gaggccggcg gccatgccga
     5941 cccggttggg gaagcgcagg cccatgacat ccaccgggcg ggcgtcgagg ggtttaccgg
     6001 cgggcagcac acggccggcg acgttgaggg ccgcgagggc ggcctcgtgg gcggtctcgg
     6061 cgtcgaagga gaacaggacg gggcgggcga tatcgtagag catccgccat tttaccgcag
     6121 tgcggcaggg cggtgtcagt cgagggcgac gcgcacctgc aggccgagat agccgctgta
     6181 gcccggcagg ttgggcagcg ccaccagatt ggcgccgacg cggccggctt caacggacac
     6241 ggcgggcagc accagcaccg gtgaggggta gccgctggcc agcaggccga aggcgccgaa
     6301 gcggaaggga ccggcggcaa ggggcatcca gcggccgccc aggtaccagg tgtcccggtc
     6361 gtagctgttg cggaagtagc cggccgaggc cacccacggg gtgccgttga agctccgttc
     6421 gaagccgaca cccggattga attcacgcca cgcccggcgg tggggctgga agtggtggga
     6481 ggcgccggag acgctgagcc agtcccggtg cggggcgttg gcgaatgcgt cggcgagtgt
     6541 gtcgaggctg tcgccggcat gcagggccgg cgcgcaggcg aggagggtcg cggcgacgaa
     6601 cgccgacggc aggtggggca tgggggattt actccggaag atcgaggtcg gcgtccgaga
     6661 ggatctgcca ctggccgccc acgcgcccct ggatggggcg gaagcgtgcc ttgtaggcca
     6721 tcttgcggct gtcgcggatc cagtagccga ggtagagata gggcagtccg agctggcggc
     6781 agacttcgat ctgccacagg atgccgaagg tgccgagccc ggcgcaggac gggtcggggt
     6841 cgtagaaggt gtagacgctg gacaggccgt cggtcaggca gtcgatgacg cacaccatgc
     6901 gcagcacgcc gttctcgcgg aattccacga ggcggctgtc cacgtggctt tgcagcagga
     6961 agtgggcgta ctgctcccgg ttgtcctggt ccatgccccc gccggcgtgg cgggtggcct
     7021 ggtagcgctg gtacagggcg tagtgttcct cgtcgaattc cagcgggagc tcgtgggggt
     7081 cgaggagggc gtgggatttc caggcacggc gctggctgcg ggtggggcgg aagccgctca
     7141 ccgggatgcg caccgggacg caggcacgac agttgtcgca gtaggggcgg taggtgaaga
     7201 tgccgctgcg gcggaagccc gagcggacga gctcgctgta gagctcggca tcgatgagat
     7261 gactcggggt ggccacctgg gagcgggcct ggcgctcgga caggtaggaa caggcgtagg
     7321 gcgccgtggc gtagaactgg agctgggagt agggatagtc cttgagcatc gggctacgtc
     7381 cagggggctt gggctccgtc ctgcggccag cgaccgggcg gcgggccggt acgctggtgg
     7441 gtgtcgacca gacgggcaaa ttccgcacgg ggaatctccc ggccgcccag cgaagccaga
     7501 tgggccgtcg tcatttgaca atctagcacg cgaaaatcgt tctgctcaag gaagcggatc
     7561 aggtgcgcga aggcgatctt ggaggcgtcc gtacggcggc tgaacatgga ttcgccgaag
     7621 aacacgtggc ccagcgccat gccgtagagg ccgcctgcca gctcgccgtc gatccaggtt
     7681 tccacgctgt gggcgtagcc gagggcgtgc atgcggccgt aggcggcctg gatctccggc
     7741 gtgatccatg tgcggccgtc gggctcacgg ggcgctgcac acgcgcggat cacggctgaa
     7801 aagtcgtggt cgaggcggac ctcgaacgcg cccttgcgca gggtcttgcg cagggagcgg
     7861 gtgatccgtg cttcggatgg aaacaccacc atgcgtgggt cgggactcca ccacaggatg
     7921 ggttcgccgt cgttgaacca ggggaagatg ccgtgccggt aggcctccag gagccagggg
     7981 ggtgtcagtt cgcctcccgc ggccaacaag ccgttggggt gccgcagtgc acggcggacg
     8041 ggcgggaatg cggggtggtc gccgagccag gggatcacgg ccgggagcgc ggtgcagggg
     8101 gtcgcctgag gggcgggccg gggtcagttg gcctgggatt gtcgcgcctg agcgctgggc
     8161 agcaggcgcc gggcaccgtc gtagcgggct gcccagtagg cgatgcgcat gtcctccacg
     8221 cgcacgaccc cgcccgcgga cggtgcatgg acgaactggt tgttgcccag gtagatgccc
     8281 acgtgggaga aggcacggcg catggtgttg aagaacacga ggtcgccagg cttgaggtcg
     8341 gagcgattca cctgttcgcc catctgggcc atttcagcag ccgtgcgagg catgccgaac
     8401 cccagggcgt cggtgaagac cttgccaacg aagccgctgc agtcgaagcc agtgaggggg
     8461 ttggtgccac ccaggcggta gggaacaccg aggaagttga ggctgcggtc catcagcgcc
     8521 ttgaccgaat cggtgtaggc gcgcagggag tcggagtagc gttcgaagaa cgcgctcacc
     8581 tgggactcgt cggcagctgc aggaggctcg gcggcgaaga ccgcgccgga gaacgcgatc
     8641 gccacaaaaa agccggtgat ggtttttttg gagggcatcg ggacactata ccgaagggtg
     8701 tctggagtgt aaagattttc gacaagccag gaatggcgcg ggttttgggt gcagtgcacc
     8761 atgtgagttg cgccacggac gaagcgtgat gtcatgggta gcatgggtcc cgcactggtc
     8821 gcgagcagca acggacagca cgccgcgcgg gaggcggacc gaaccggtcg ggatgtctcc
     8881 gtcaaagggc ccttcggcgc gccgatccgg ggcgctcgtg ccagtcgcac caatttgacg
     8941 atctgttcgg ccagtccctg gcgggactca gttgaacagg tgaaggatgc cgtccagccc
     9001 ggcgtgactg agggcgtggg tcgcctgcgc gcggaccacc ggttttgcgt gataggcgac
     9061 gccgatgccg gcttcggcga gcatggccag atcattggcg ccgtcaccgg cggcaatgac
     9121 ggtgttccgc gcgaagccgt ggtgttcgcg caccagacgc agggcgcggc gcttggcgtc
     9181 gccgtcgatg atctcgccct tgacgcggcc cgtgagcctg cccgagcgga cttccagctg
     9241 gttggcgaag gcgtagtcga agcccaggcg cgccttgagc cggtcggtga agtaggtgaa
     9301 gccgccggat accagcaccg tgacgacgcc ggcccgtttg aggccggtca tcagcgcttc
     9361 cgcgccggga ttgaggtgca ggcgctcctg atagacccgc gccagggccg cctcgggcag
     9421 gccttcgagc agcgccacac gtcggctgag ggacgcgcgg aaatccagtt cgccccgcat
     9481 ggcggcttcg gtgatggcag agacctcggc cttgaggccg tgcatgtcgg cgatctcgtc
     9541 gatgcactcg atggtgatga gcgtcgagtc catgtccgtg acgaacagcc ggtagtcctg
     9601 aaggcggcgc ccttccggca cttcggcgca gtcgagcccg tgggcctcgc aataggcgga
     9661 cacctcgggc gtgaggtggg cgtcgaggag gcggaaggcg ttggtcccga ggcgaccgag
     9721 cccctgggaa ccggcgagcg tcgacagatc cttgagggcc cgcgcttcga gtttcgggcc
     9781 ctggacaacc agattcatca ggcgccccgc tggggcagca cgtcccggag gatgtcgcgg
     9841 gtgttgcgga tcatgtccac ggtggtgtcc cagctcacgc aggcatcggt gaccgagcag
     9901 ccgtagcgca gcgtggacag gtctgccggg atgggctggt tgccggcttc gatgaagctt
     9961 tcgatcatca gaccgacgat ggagcggttg ccctcgcgga tctggtgggc gacatcacgc
    10021 atgaccaggg gctggtattc cggattcttc caggaattgg cgtgggagca atccaccacg
    10081 atgttggccg ggatcttggc cttggcgagg gccttttccg ccagggagac ggacacggtg
    10141 tcgtagttgg gacggccgcc gccgccgcgc agcaccacgt ggccgtagcg gttgccgcgg
    10201 gtccggatga tggatgactg gccctggccg ttgatgccga ggaaactgtg gggattggcg
    10261 gcggagatga tgccgttcac ggccgcatcc agggaaccgt cggtgccgtt cttgaagccg
    10321 accggggtgg acaggccgga ggccatctcg cggtgggtct gggattcaga ggtgcgggcg
    10381 ccgatggcgg tccagctgat gaggtcgccg taatactggg gcgcgatggg atcgagggcc
    10441 tcggtggcag caggcaggcc gacttggttg acctggagca ggaagctgcg ggccttttcc
    10501 atgccttcct cgatgcggaa ggagtcgtcc atgtaggggt cgttgatgta gcccttccag
    10561 ccggtggcgg tgcgcggctt ttcgaagtac acacgcatca ccagcacgag ggtgtcggcg
    10621 acttcgtcgg cgagggtttt gagacgccgg gcgtagtcga tgccggccac cgggtcgtgg
    10681 atggagcacg gtccgaccac cacgaacagg cgcggatcct gatgatccag gatggcctgc
    10741 agtgtgcggc gcccggtcag cacggtctgt tcggcagcgg ggctgagggg tacgcgggtg
    10801 gtgatctcgt cgggcgacgg catccggtcg aaggccgcga tgttgaggtt gtcggtctgg
    10861 gcgctggaca tggcatctcg cgaggggtgg agcgaatccg gcattctaac cgcagacggg
    10921 gacgaaatcc caagggctga tcggttcagc gcccgcgacg cagaggatgg cgggcgaggg
    10981 cgaggacgct gggcaggcgc acgtccaccc acgcgggacg gcgcaggctg cggctgatcc
    11041 agacggtctt catgcccagg cggcgggcgg tgcgcaggtt gtcggcgctg tcctcgacga
    11101 ggatgcagcg cgcgggctgg aggcgctgct ggcgcagcag gtggcggaag ccgcggatgt
    11161 cgggcttggg gtggaagcgc atctgttcga ccgcgcatac atcgctgaaa tgatgacgga
    11221 cgcccagcac gtcgaggacg gcctcggcat aacggcgcgg gccgttggag aagacgatct
    11281 tgcgtccggg caggcgccgc agcaggtgac tgagggctgc atcggccaca accatgcggt
    11341 gcagttcggg gaactggtgg gtgtcgtgga ggaagtggtg gggattggtg ccgtggtggc
    11401 gcatcaggcc ggtgagggtg gcaccgtagc gccgccagta gtcgacgcgc aggcggttgg
    11461 cggcagcctc gtccagggcc agatggcgca tgaggtaggc cgtcatgctc cggttgatgt
    11521 gggggaagat gtgcggactg gcgttgtgca gcgtgttgtc caggtcgaag agccagacgg
    11581 gcttcatggc gctcgggccg gactcagcgc ggtggccgtt cgaagcggac gatggcgcgt
    11641 ccgtcttcgg cgactttggg ggccaccttc caggcgtggc catagaccac ttcgacggtc
    11701 gcgggcagac gtccgttgcg acgcagtgcc tcgtaggcgg cacgcaggcc ggaccagtgt
    11761 cccttgccgc tgagcccccg ggggcggccg gccgatgcgc aggcggagcc gctcaggcgc
    11821 aggtcgcgca gcaagtcgtc gagggaggcg taggtgaccg tgagcatctc catgtccatc
    11881 accgggtcgg agaagccggc ctgcacgagg gcatcgccca ggtcatgcat gtcgatgaag
    11941 cggtgcaggc gcgcgccgtc cggtccgggc aggacgctgc gcagttcgcg cagggtgtcc
    12001 ggcccgaggg tggagaacat gagcatgccg cccacctcca ggacccggtg catctccttg
    12061 agggccggca ggggatcgtg cagccactgg agcatcaggt tggaccacac cagcgatacg
    12121 gcgccacgct tgagcggcag ggcgcgtgcg tcggcgcaga ggaagccggg ggcgctcggg
    12181 gtgccgcggg atcccagcag gcgctggaaa aagccctgct gcggaccgtg ggcgtcccgc
    12241 gcgagggcga gcatcgtccg cgccacgtcc acggcgaggc gcggcacgtc cgggtagcgg
    12301 gcggcgaagg cgggcaggtc tgcgccaggg ccgcagccca ggtcgaggat gcggctgggg
    12361 gcgatgcgga tgtagtcgag gcgttcgccc atgcgccggg ccacttcccg ggcgagggcg
    12421 tccacctcgg cgtaggtcgc ggcggcccgc ccggcccggc gccgcacgag gcgcaggtcg
    12481 agctggaaat ccggcgcggt catcggcggc ggaaactcag gcggacttga ggccgaggcg
    12541 ggcgaagagc cggtcgtcgg cgtcggcgtc cgggttgtcc gtggtgagca ggcgctcgcc
    12601 gtagaagatg gagttggcgc cggccaggaa ggcgagggcc tggaagccgt cgcccagttc
    12661 ctggcggccg gcggacaggc gcacgtagct ttcgggcatg gtgatgcgtg ccgcggcgac
    12721 gacgcggatg aagtcgaagg ggtcgatggc ctcagcgttg cccaggggcg tgccttcgat
    12781 ctgcaccagg ttgttgatgg gcacggaatc cggcggcgga tccatgttgg ccagttcggc
    12841 gatgaaggcg gcgcggctct tgcgggtttc gcccaggccg agaatgccgc cgcagcacac
    12901 gttgatgccg gccttgcgca ccttgtcgag ggtgtcgagg cggtcctgca gggtgtggga
    12961 ggtgatgatc tggccgtaga attcgggcgc ggtgtcgatg ttgtgattgt agtagtcgag
    13021 gccggcgtcc ttgagctgtt cggcctggcc atccttgagc atgcccaggg tgacgcaggt
    13081 ttccaggccc atggccttca cttcgcggac catgtcgagc accggggcga ggtccttttc
    13141 cttcggtccg cgccaggcag cgcccatgca gaagcgggtg gcacccttgg ccttggcggc
    13201 gcgggcgttg tcgagcactt cgccgagggg cagcagggtc tccttttcaa ggccggtgtc
    13261 gtagcgggca gactgggagc agtagccgca gtcttcggag cagccgccgg tcttgatgga
    13321 gagcagggtc gagcgctgga ccgcgttggc gtcaaaatgg gcgcgatgga cctgctgggc
    13381 gcggaaaatc aggtcattga agggcagcgc gaagagggcg tccacctcgg ccacggacca
    13441 gcgtttgcgc ggcgtggacg ggggggcggt ggtgaggggc tcgtgggggg cgacggtggc
    13501 ggtcatgggc atgcaccctt atagtctggg ctgtaattca aaattacgaa gccttttagt
    13561 tttactgatg gggtctcgcc ttgtcaaatc aaagcgatag atggcgacat tggctggggt
    13621 ggctgttgcc ccaggcctgc ttcgcgtgcg gtgcgcccag cggcgacggc gtgctgtgcg
    13681 atgcctgtta cggcgccctg ccggggcggg actgcccgcg ctgcccgcgg tgcgccctgc
    13741 cacagccagg cggcggcctg tgcggtgcct gtctgcgtga gacgccgccc ttcgaggcca
    13801 cccgcgtggc gctggactac gcctttccgg ccgacagcct ggtgctggcc ctcaagtatg
    13861 cccaccggct cgcgacgacg cggctcttcg ttgatctgct ggccgcagcg ccacgtcccg
    13921 aggcggatct ggtggtgccg atgccgctgc acccggcgcg gctggcacaa cgcggcttca
    13981 accaggctgc cgagctggcc cgtccgctgg cgcgacactg gcagttgccg gtgcgccttg
    14041 acggggtggt gcgggacgtg aatacggtcc cccaggcggg cctgccatgg aaggcgcggg
    14101 cggccaacgt gcgcggcgcc ttccgcgtga ccggacgcct ggacggccgt cgcgtggtgg
    14161 tggtagacga cgtgatgacc accggtgcaa cacttgcgga actggcccgg gccctcaagg
    14221 ccgctggtgc ggtgtccgtg gagaaccggg tgctggcgcg cacgccgccg ccggcttgat
    14281 gggggagggg cgtgcgggca caatccgcgc ccatgctcca tgtcgtcctc catcagccgg
    14341 aaatcccgcc caataccggc aacatcatgc gcatgtgcgc caactccggc gcccgcctgc
    14401 atctggtgaa gcccctcggc ttcacgctcg aagagaagca gctcgtccgt gccgggctgg
    14461 attaccgcga tctcgccgtg gtgacggtgc acgaagactg gcctgcgtgc cgggcggcac
    14521 tggcagggcg gcgttgcttc gcgctcactt cccacggcgg ccggcattac ggtgatgtgg
    14581 cgtttgctga cgaggatgta tttctcttcg gcgccgagtc ccgcgggctg cccgccgagc
    14641 tgcacgcggc attcgacgat gaacaccgcc tgcgcattcc catgcggccg ggcaatcgca
    14701 gcctgaacct gtccaacgcg gcggcggtgg tggtctacga ggcgtggcgc cagctgggct
    14761 ttgccggcgg gatctgagcg gcgcagggag ggctattcct gcttgggatc gcgggcgagg
    14821 agctgctcaa ccgcctcacg ggcacccaga ccgccatgga gcacgccgtc cacggctgcg
    14881 gtgatgggca tgtccacacc cagccgggcg gccagggcgg cgacttcccg cgcggtgctg
    14941 acgccttcgg ccacgtgtcc gagggtggcg aggatgtctg gcagagtctt gccttcggcc
    15001 aggagcagac cgacccgacg gttgcgggaa aggtcgccgg tgcaggtgag gaccagatcg
    15061 cccagaccgg cgagtcccat gaaggtctcc cggcggccgc cgagggcgat gcccaggcgg
    15121 gtgatctcgg ccagcccgcg ggtgatgagg gcggcccggg cgttgaggcc gaaacccatg
    15181 ccgtcggcga cgccggccgc gatggccatg acgttcttga ccgcgccgcc cacttccgcg
    15241 ccgaccagat catcgttggc gtagaggcgc aggcgcgggt cgtggagcgc attcacccaa
    15301 tgacgcgcga aggccaggtc gcgggccgcc agggtgacgg cggcgggcag gcctttggcc
    15361 acttcagcgg caaagctggg accggtcagc acgccgcagg gggcgtctcc gcccagttca
    15421 tcggcgacga tctggtgggg caactgggcg gtgcccgcct cgagaccctt gcaggcccac
    15481 agcagcggcg tacccggggc cagatcgtgc agggcgcggg ccgtggcgcg caggccggcg
    15541 aggggtgtga cgaccagatg aaggtcggcg cgggcggcct cggcaaggct gtcgctgggg
    15601 tgcagcccgg cgggcaaggg gatgccgggg aggtagcggc gattttcgcc gtcactgcgc
    15661 agatcggcga tctcgcgggc ttcgcggctc cacagggtga cgcggtgctg gcggctgaag
    15721 gcgatggcga gggcggtgcc ccaggcgccg gcgccgaaga ctccgatgtg catcacaggc
    15781 cccagacctg ggtcatgcgg atgaagcccg tgctgccatc ccgatggcgg accttgaccc
    15841 atccgtcggc gggtgcgtcg gccagttcca ggacaaggtc cttggccgcc tcgaaggcca
    15901 ggggggcatt cccatccggc ttctggcgga tctcggcgcg ggcggcggtg acgatcaggg
    15961 tgcgtttctc agacagctgg cggcgttcca gccagagcag gctgccggcc ggttcgcgca
    16021 ccttgaccca gcgatcgacg ctgacgacca tctccacggg ggtgtagcgc tggatcacga
    16081 agagcggttt gccctgtttg gaaggcgcat cgtataggat ggccggctcg gccagcgacc
    16141 ggaaatcgga ggcgctggcc ggggtggccg ccagggcgca gggcagcgcg aggagcgctg
    16201 cgacagtcag gcggcgcagc ggcattactg gaccggaatg tcggtgccgg cggaagcctg
    16261 gcggctctgc tggaacaggg cctcgaagtt caccggcgcg aggatcacgg gctggaagcc
    16321 ggcacggctg acggcgtcgg agatggcttc acgggcgtag gggaagagga tgttggcgca
    16381 gccgatcatc atcagcggct ccaggtcttc ctggggcacg ttgcggatct ggaagatgcc
    16441 ggcctgggcg acttcgacga ggaacacggt cttgtcgtcg ggcagcttgg cggtgaccgt
    16501 gacggtcagg ttgacctcga acacgccgtc gtccaccgcg gtgctttcgg tgcgcagctg
    16561 cacgccgatc tgcggggctt cgcgctccag gaagatctgc ggtgcgttgg gcacctcgac
    16621 ggagagatcc ttgacgtaaa gcttttcgat ggagaagacg ggttgttgcg cggtttcgga
    16681 catggtgggt tttccgtttg cgtcggtggg atgaatgaaa tgatgggggc cgctcaggcg
    16741 ccttcaccgc gcagcagcgg atcgaggcgg ccctgatggt ccagttcgaa gaggtcgtca
    16801 cagccgccca cgtggaaatc gccgatgtag atctgcggca cggtgcggcg gccggtgcgg
    16861 gacatcatct cgtcacggcg gaccggatcc aggtcgatgc ggaccttctc gatctcggtg
    16921 acgcccttgc gccgcagcag cccttcggcg cgcacgcaat agggacacac gccggtagcg
    16981 tacatgagga tgtgtggctg catgatgact caggccttct tcttggactt gccgctgcgg
    17041 cccaggggca tgttggcctg ctcccaggcc tggatgccgc cgtccaggtt gtgtacccgg
    17101 gtgaaaccgg ccttggtgag cacgttgcag gcgttgaccg agcggccacc gctctggcag
    17161 ttgacgatga ccggcttgtc cttgaaggat tccagctcgc caaggcgctt ttcgagatcg
    17221 gccaggggga tgtggcgggc gttgggcacg tgaccggcgg caaattcgcc cgcgtcgcgc
    17281 acatcgatga cgagggcgtc ctcgcggttc atgagctgcg tcgcctgggc cggggagatg
    17341 ccctggccgc cgcgttgcag ggaggtggcg acgagcaggc tgccgctgac tgcggcgagc
    17401 agcgcccaat accagttttg ctggaggaat tccacgaatc ggactccgga acgggaaggg
    17461 gttgaacggg ggtggacctc agcctttggc cgagtcgccg cagaagactt cgcgcatcat
    17521 gacgatgagc tgcagggtgc gggtgtcgcc gacgcggtaa tacacccggt tggcgtcctt
    17581 gcgggtgagc agcacaccct tgtcgcgcag gatggccaga tgttgggaga tgttgctctg
    17641 ggaggtgccg acggcttcga cgatttcctg cacgcacgct tcctcggcgc cgatgacgca
    17701 gaggatcttg aggcgcaagg ggtgcgagat ggccttcagc gcacgggcgg cggtctcgat
    17761 gtgttcctgc ttggcaatca ggtcgacgat ggggctggtc acgaggctac atctgctctg
    17821 ggtctgcaat attataaaat accatctttc ccgctccggg tgcggcgcga tccttttcga
    17881 cgggccctcg ccgaccgccc gtcgcggcca ccaggacggc cgcgcgccct gcaggcgggg
    17941 aacctttcgg gtttatactc ctcactcctg acggatcccc cggaatggcc ggtgtgcggt
    18001 gctcgggagt cgccgacgtg gtccttgccg cgtcccccac ggagagttta tgcgcagcaa
    18061 gttgcaacag gccggtctgg tgttcacggg gctgtgtgcc ggcatcctca tcagtctcaa
    18121 tttctccgcc aacgcggaca agaacagcct gccaccgctg ccggtggagg agctgcgcaa
    18181 tctggccgac gtcttcaacg ccatcaagca gggctacgtc gagccggtgg acgacaagaa
    18241 gctgatcacc tatgcgatca gcggcatgct ctccaacctc gacccccatt ccacctacct
    18301 ggatccggaa gccttcaagg aattgcaggt gggcacccag ggcgaattcg gcggactcgg
    18361 catcgaggtg ggctccgagg atggctacgt caaggtgatc tcaccgatcg aggacacgcc
    18421 cgccttccgg gccggcatca aggccggcga cctgatcatc aaactcgacg acaccccggt
    18481 gaagggcatg accctcaacg acgccgtcaa gcgcatgcgc ggcaaaccca agaccagcat
    18541 cgtgctgacc atcatccgca agggcgaggc caagcccctg acggtgaccc tggtgcggga
    18601 gatcatcaag gtccagagcg tcaagtcgaa actcgtcgag ccgggctacg gctatgtgcg
    18661 cgtcgtgcag ttccaggagc agacggccgc gtccctggtc cagcatctcg accggctggt
    18721 caagcagggc gaactcaaag gcctgatcct cgatctgcgt aacgatccgg gcggcttgct
    18781 caatggcgcg gtgggggtct cggccgcctt cctgccgccc aaggcgctgg tggtgtccac
    18841 cgacggtcgc accgatgacg cccgtcgcaa gtatttcgcg gtgcccgacg actacctccg
    18901 cggcagccgg gatgactaca tccgcaatgt ccctgcgagc atcaagacag tgccgatggt
    18961 ggtgctcgtg aatggcgggt cggcgtccgc gtcggaaatc gtggccggcg ccctgcagga
    19021 tcacaagcgg gcggtggtga tgggcaccca gagcttcggc aagggctcgg tgcagaccat
    19081 tctgcccctc aacagcaacg ccgccatcaa gctcaccaca gcccgttact tcacgccgag
    19141 cggacgttcg atccaggcca agggcatcgt gccggacatc gtggtggaag agacgcccaa
    19201 cggttcccgt gaacgcctgc gcgaggccga tctcgaccgt cacctggtca atgccggcga
    19261 gccggacgcg ccgaaatcgg agacgcctgc gtcccgcaag gatccggccg gcaagccccg
    19321 ggacaaggac aatcgggaca acaaggacaa gcccaaggac gaggcggacg aggtcaagcc
    19381 ggcctttgaa atcgccggca aggacgacta ccagctggcc caggcggtga atttgctcaa
    19441 gggcctgcaa atcctccaga atagcgccaa gtaaagggct ctttccggga ggcgccatgc
    19501 gtcgcgaaac cgcttacaag cttgccggcc gcaagcatga tcacgcctcg catcacgcgg
    19561 gcagcggcct gaccaagtcg cgggagatcc gcttcaagga tctccctcct ggccaggccg
    19621 tccaggcccg gcgttcgctg gcggcgctca agggcgtacg ggtgacggac ggcaagcatc
    19681 ccctcgagct cgtcgttcat tacaacgtgc tcgattacac ccttgaactg ctggaagtgg
    19741 ccctgcagaa cgccggcttc catctggatc ggcccctgct ggtgcgcctg caccgggcgc
    19801 tgatctacta cgtggaggag acccaggtgc ataacctgcg gtcgcccgag cggctcatca
    19861 agcagtccca cgaagtctac gtcaaggcct acgcggcgca ccctcacggc gatcacgacg
    19921 acacgccgct ggagctgcgc gagtacaagt aggcccctcc cgtcccgtcg cccgtggggg
    19981 cccacgggtg cttcacgccg ccccgtgccg atgaacgacg atcagctcct gcgctattcc
    20041 cgacatatcc tgctgcccca gattggtatc gaggggcagt ccgccatcgt gtcggcgcgc
    20101 gtgctggtgg tgggcgcggg cggactgggc tcgcctgcgg cgatgtacct ggcggcggcg
    20161 ggggttggta cgctggtgct ggctgacggc gataccgtgg atctgaccaa tctgcagcgt
    20221 cagatcctgc atggcgaggc cagtgtcggg cgtctcaagg tggattccgg acaggagacg
    20281 ctgtcccgac tcaacccgga aacgcgcgtc gaatccctgc cgcgtcgact cgaaggccgc
    20341 gagctggacg agcaggtggc cttggccgat gtcgtgctgg attgctccga caacttcgcc
    20401 acccgccacg ccatcaaccg ggcctgtgtg gcccatcgca cgcccctggt gtcgggcgcc
    20461 gcggtgcgtt tcgacgggca gctctcggtg tttgacctgc gtactgacgc cagcccttgc
    20521 taccactgcc tgtttcccga gggcgaggac gtggaggagg tgcgttgcgc ggtgatgggg
    20581 gtcttcgcac ccctcaccgg catcgtcggg accgtgcagg cggccgaagc gctcaagctg
    20641 ctggccggct gtggggagac cctcaccggc aggctgctgt tgctggatgc gctggagatg
    20701 gactggcgct cggtgcgcct cacgcgggat cccacgtgcg cggtgtgtgc gtcgcgctga
    20761 ccacggccgg aacgaccttg cctgtgttca ggcaggcttc ccgatcagcg ccttccagag
    20821 cagtttgaac ccgagtttct gctgggccca gaagtggtcg cgggcgttgg attcctccgg
    20881 cgatgcctcg tgcaggccgg tggcgggcca gcggcgttcc aggtaaagcg tcccgaagag
    20941 gacatcccac acgggcagca ggatcgcgaa gttgcagccg tggcgatagc gcggaccggc
    21001 gggcaggtcg agggcgtgat gccagcggtg atacagcgga ccgaccacca ggtagtcgcc
    21061 gatgggcccg aagtcgaagc gcgtgttggc gtgggagagg ttttccacca ggcgcagggc
    21121 gatcagcagg ccgatgaagt gcccaggcgg cacgccgatg aacagggcca ggccggcaaa
    21181 ccacagaccg gcgatgaggt catccgccag atggttgcgg gaatcggtcc agaaggacat
    21241 ctgccgctgg gaatggtgga tgccgtgcag cgcccaccac cagcggaaca catgggacag
    21301 gcggtggcgc cagtattcgg cgaaatcgag gatcaccaga tacagcagga agctgaccaa
    21361 cggatgatga tccagggccg gcagcagatc ttccagctga cgcgggagca ggtcgtggaa
    21421 gcgcagccag ctttccacct cggcgaacag cggggcgagc agcagaaaca ccgccagcgg
    21481 cacgatgccc aggcgattga ggaaggtgta gagcatgtcg acccgcacgg cgcggcgcgc
    21541 ggcccaacgc tcggccggcc acagggcttc gagagggcgc aggaggaggt aggcgacggc
    21601 cagctccagc aggccgagga gaacgaattc gacaccgtcg aagcccaact ccacaaacga
    21661 gcccaatccc agtgcaaaca tcaccggctg cacgagggcg tcgaagagcc aggcctggag
    21721 ggcgatgaag gcgtcggtga gggactggat catggccgtt ccggaatcgt ggcgaagcag
    21781 aagcccttgc gctggagttc gccgatgagt gggtcgagca tcggggcgaa ggggtccttg
    21841 cgtgaccaga tgcccaggtg ggccatcagg atgtcgccgt ccttcagggt gtcgagggcg
    21901 cggcggacca gcacttcgtt ggggaaggcg tcggacggca gctcgtcgcc gaggaagccg
    21961 gcgggcgccc agcccacgtg ccggtaaccg caggcctcgg cggcctggag ggtgaggggt
    22021 gtggtgcggc cgccgggggc gcgccacagg gggtcgaggg ggcggccggt gagctcggcg
    22081 aagcgccggt cgggctggcg gagctcctcg cagacctgcg ccgaatccag ctggacggtg
    22141 ctgccgtcca tcaggcggta gctgacgcgg ccgtcggcac cgtccttgcg gaaggagccg
    22201 tggcgccagg tgtggctgcc gaaggcgtgc ccctcggcga cgcgggcttt ccagaagggc
    22261 gcccagctgg cgtcgagggc atggtcaccg cgcagggtct tttcctgggc gacgaagaag
    22321 gtggcgcgga tgtggtgacg ggccaggatg tcggcgatcg cctcggcgtg gcgcatgttg
    22381 cccgtgtcga gggtcaggta cagggtgccc gccgggcagt cggccttgcc ggcgccggcg
    22441 acggccggca cgagggctgt cagggccagg gcggcgaggt gtttcattga cgcggggcgt
    22501 ggttcaggaa gaacacgccg tggggcgagc ggccgaccgg gatggagcgg gcgagcttct
    22561 tctgctccag atccaccacc tgcaccttgc gggcgaagcg ggcggtggcc cacagctcct
    22621 tgccgtcggc acgaatctcc atgtcgtccg ggccgcccgg cacgtcgtat ttttcgacga
    22681 cgctgagggt ctgcatgtcc accagcgaga tgctgccgcg caccgtgcgg ttggacacga
    22741 atacgtggcg gccgtcgccg cgggcgagga agttgtgggc ggccttgtcg gtgacgatgc
    22801 gcttgacggt cttgcgggcg cgccagtcga tgacctccac gtagtcggcg cccatgatgc
    22861 cgaccagtag gtatttgtcg tcgggtgtca tgtggatgcc ggccggggtc gggccgacgg
    22921 ggagcttcca gaggggctgc tgggtcttga ggtcgatggc gaggacttcg ttggaatcct
    22981 gcagggtaat gaagacgaac tggctggcct tgtcgaaggc catgtgggag ggtgttttgg
    23041 cggcgggtac gcgggccacc agcttgaaat cggcggcggt gtagagatct acccggtcga
    23101 ggcgcaggga gttggccacg aaccatttat tgtccggcga gaagccgagc tggtacgggt
    23161 cggagatgcc gtgcaggcgc ttctgctcct tgccgttgcg agggtcgaag aacaccagtt
    23221 cgttggagat ggcgcaggca acgatcactg agctgtcgtc cggcgtggac atcaggtggt
    23281 gaggttcctt gcaggcgggc gcacggccgg tttccttgta ggcgacggtg tcgacgaagc
    23341 tgacggtctc gtcggcggag ttgaggacca gggctgtctc ggcacaggca agggccggca
    23401 ggacgctggc agtcagggca agcaggaagc ggggaaggcg catggagcgg ggacgggtgg
    23461 atcgaaaagg gctatgactc taacgcaatg gaatcgacgg gcgttgaccc aggccaaact
    23521 ccggcgcgga gggatgtcaa attcacggtc gggacatccc gcgctcaggc ggggcgggcg
    23581 acgatgcgca gatccggccc gatctggcgg acgtcgcgga agttcagccg gcgctgatcg
    23641 gccagagcat cgagcgcggg caggttgaac agtccgcggg ccgcatcgcc gaccaaggtc
    23701 ggggcgaggt agagcaccag ttcgtccacg cagccttccc gcagcaggga gccgttaagc
    23761 ttgaagccgc tctcggcggt gatctcgttg aggccgcggc ggccgagttc ggcgagcagg
    23821 gccggcaggg ccaccttgcc ggcgtcattg gggaggatca atacgtccgc gccccgtgcc
    23881 tgcagcgcgg cgatgcgggc cgggtcgtcc acggcgccgg cgatgagcac cgggccgccg
    23941 tccaggacgt gggcgtggag gggcgtctcg agacgggcgt ccacgacgat gcgcaggggc
    24001 tggcgttcgg tgggaatggc gcgcacgttg agctgcggat cgtcgtcacg cacggtgccg
    24061 ataccggtga gcactgcgca ggagcgggcg cgccagcggt gggcgtcgcg gcgggcgtcg
    24121 gggccggtga tccactggga gacaccgttg tggagggccg tcttgccgtc cagcgtggca
    24181 gcggccttga ggcgcagcca gggccgaccg cgggtcatgc gcgagacgaa gccgatgttg
    24241 agttcccgtg cttcggtttc gaggacgccg cagtgggtgg cgatgccggc ctcacgcaga
    24301 cgggcgaggc cgcggccggc gacgaggggg ttgggatcct ccatggcagc cacgacgcgg
    24361 gcgactccgg cccgcaccag ggcgtcggcg caggggggcg tgcggccgtg gtgggcgcag
    24421 ggttcgaggg tgacgtaggc cgtggcaccg cgggcccgat caccggcctg ggcgagggcg
    24481 tggacttcgg cgtggggctc gccggcgcgg cggtgccagc cttcgccgac cacctgcccg
    24541 tcgcggacga gtacgcagcc gacccgtgga ttgggggtgg tggtaaacag gccgagttct
    24601 gccagctgga gggcgcgggc catccaccgg gtgtcatcga cgctggcaga catgatcagg
    24661 acgtggagcg ggggcgtccg cggggacggg gcgagacttc gcggatgact tcggagaact
    24721 cgtccacgtc ctcgaaggcg cgatagaccg aggcaaagcg gatgtaggcg accttgtcga
    24781 gcttcttgag ctcgcgcatg accagttcgc caatgagctc tgaggcgact tcccgttcgc
    24841 ccagggacag cagcttggct tcgatgcggt cgacggcggc ttcgacgctt tcggccatga
    24901 ccggccgctt gcgcagggcc agggagaggc tgccgaggag cttgtcgcgg ctgaagtcgg
    24961 cgcgactgcc gttgcgcttg accacctggg gcatcttcag gtcgacgcgt tcgtaggtgg
    25021 tgaagcgctt gtcgcaggtc aggcagcgac ggcggcgccg cacgatgtca ccgtcctcgt
    25081 tctcacgggt gtcggtgacc tgcgtgttct cggcgctgca gaaggggcat cgcatggagg
    25141 ctgctgcctg tcagaggctc atgcgccgta gaccgggaac ttgcggcaca gttcggcgac
    25201 cttgctgcgg acggcggcga gggtggcttc gtcgttggga ttgtccagca cgtcggcgat
    25261 caggtgcgcg acgcgttccg cttcgatctc ggtgaagcca cgggtggtca tggccggcga
    25321 gccgatacgg atgccggagg tgacgaaggg cttctgggga tccttgggga tcgcgttctt
    25381 gttcacggtg atgtgggcgc ggccgagggc ggcttcggct tccttgccgg tgatgttctt
    25441 ggcgcgcaga tccaccagga acacgtggct ttcggtgcgg ccggagacga tgcgcaggcc
    25501 gcgctcctcg ccgaggacgc gggccatcac gcgggcgttg gcgatgacct gttcctggta
    25561 gttcttgaac tcgggcgtgg cggcttcctt gaaggccacg gccttggcgg cgatcacgtg
    25621 catcagcgga ccgccctgca gacctgggaa gatggcggag ttgatggcct tctcgtgctc
    25681 ggccttcatc aggatcacgc cgccgcgggg gccgcgcagg gtcttgtggg tggtggaggt
    25741 gaccacgtcg gcgtggggca ccgggttggg gtagaagccg gcggcgatga ggccggcgta
    25801 gtgggccatg tccacccaga agatggcgcc gacttccttg gcgatctttg cgaagcgctc
    25861 ccagtcgatg cgcagggcgt aggcggaggc gccggcgacg atgatgcgcg gcttggcttc
    25921 gcgggccagt ttttccatcg cctcgtagtc gatctcttcc ttgtcgtcga ggccgtaggc
    25981 gaccacgtta aaccacttgc cggacaggtt gaggggcatg ccatgggtga ggtggccgcc
    26041 ttcggcgagg ctcatgccca tgatggtgtc gccgggcttg gcgaaggcca tcagcacggc
    26101 ctggttggcc tgggaaccgg agttgggctg cacattggcg gcgtcggcgc cgaacagggc
    26161 cttgaggcgg tcgatggcga gctgctcggc cacatccacg tgttcgcaac caccgtagta
    26221 tcgcttgccg ggatagcctt ccgcgtactt gttggtgagc tgcgagccct gggcttccat
    26281 gacggcgcag ctgacgtagt tttcggaggc gatcagttcg atgtgatctt cctgacggcg
    26341 gttttcggcc tcaacggtct gccacagttc ggggtcgacg cgagcaaggg tgttttgctt
    26401 ggaaaacatg gcaatccagg gggttagtgc aaaaatgaag tgtaacacgc ccccccgggc
    26461 gttgccgaac ccggggtttc ccggatcaca ggcggaagtg gctgacgcgg gcttccagtt
    26521 cgtcggcgag ttgttccagc tgggtggtct gggcgtggct gtcgcggatg atctggttgt
    26581 tctgttcggc catgcgggcg atctgctcta cgttgcgggc cacctcctgg ctcgcggaac
    26641 tctgctcgag ggtggcgtcg gcaatgtcgt tgacggcgcc ggtcgtcctc gcgctctcgc
    26701 tgcggatggt ctcgagcatg gcgcccgaag cagtggcgac agccaccccg ttctgaacgc
    26761 cggttgtgat gccctggatg gtgtcgacca cacgctgggc ttcgtcctgc accagggtga
    26821 ccgtctcgga gatctctcgc gtggaacttg ctgtgcgttc ggccagcttg cgcacttcgt
    26881 cggccacgac ggcgaagccg cggccgctct caccggcccg tgcggcctcg atggcggcgt
    26941 tgagggcgag gaggttggtc tggtcggcga tgtcgttgat gatccggcca atgttggaaa
    27001 tgcgggcgga gcgctgggac aggctgttga cggcggcgct ggcggtgtcc atgtcggcgg
    27061 caatgcggcc catgccatcg atcatctcgc gcatgcggtg ctggccgtcg tcggcgtggg
    27121 cgctggcgtc ctgggtcatc tcgcgggcga gggcggcgct gtcggaaacg tgggtgatgc
    27181 tcacggacag ttcttccacg gcggcggcaa gacccgaggc cgactcgctc tggcgggcgg
    27241 tgctggtgac gatgctttcc gagtcggcac gcagggaatg agcggccccc ttgatggcat
    27301 tggcggtgtg ggtgacgccg ccgatcagac cggccatctg gcgggtcgtg ctgtccagtg
    27361 cccgggtgat gagttcgact tcgttgcgag ttgggtccgg gacggcgggg aaggcgacgt
    27421 tgagttcgcc gcgaccgatg cgttcggtga cggcgaccag ttcgcgtatc ggcgcaatgc
    27481 gtgaacgggc gatgagatag agcacggcca ccagcagggc tgcgccggcg gtgcagagca
    27541 ggatcagcac ccggagcagt tcgcgggcat ggacggtgta ctcgtccaca aagctgccgg
    27601 tggcaacgat ccagccccac gacggggccg tgtcgaaggc gaccatcttg gtcttttcgc
    27661 ccccgccgtt ggctggccag tcgtaggtca gggtgccctt gcccaggcgg atctgctcgc
    27721 cgaccacgcg gctcagaacc gggttgttga gttcgttgag cttcttgccg ctcagggtcg
    27781 ggtgaaagac gacctcgctg gtggcggcgt ctgcgcccgg ggcgatgaca taggtgtagc
    27841 cggtggcgcc ggagcgggtt tcgcccagaa ccgcaaagag ccgtgccatg tcgctctgaa
    27901 tgtccacacg tgcggcgagg gcgccgatga ccttgccgtc ggcgtcgcgc atgggatcga
    27961 tggcgctgat gaagaaggtg ccattgcgtc gtaccacgcc ccggtaggat ttgccctcca
    28021 ggagggctcg cgtttccttg ccgtcacgcg gcagcggtgt gccttcggtg ctcttgccgt
    28081 ccttgtcgcg gagcagggtg gcggcgcgca cgaattcgtc gcccacccgc agcagcaccg
    28141 cgggatctgc gtcgatctgg tcgcgcaggg tttccaggag ggccttgttg ccattgagga
    28201 tacgttcgcc gctgcggacc acggggacct ggtagctgcc cgatgccatc gtgtcggggc
    28261 cgatgcgcag gggcccgaag gcctgctgga cccgcccgag gctactgccg gcgcgggcca
    28321 cggcgccggc gtgattgagc tcgagcatgc gcacgacgcc ctggatctga tggccgagct
    28381 cgtcgcccgt cttggccaga tcggctttct cggcgaagta ggtggtgaag gtgaccaggg
    28441 tgccgaagac gatcaggctg atgaggcccg cggcgagggc gatctgatgg acgagggaca
    28501 ggcggttcag tctggacatg gcgattctcc cgcgcccgtc gcgcaagctg ggcgatgtgg
    28561 ccgggaaaat cggcggtcca tcagaaaact ttaatttgcg ctgcggtcct cagctgttgg
    28621 gcaattcatc gagggcgccg tgcaggtggc tctggcgggc ccattcgatg aggctccagc
    28681 ggccctcgtc ccaccccagc cagttgaggg ccgcattggg aatggtgaag tcgcgcggcg
    28741 tgtcgagggg ctttccggtg gccaggcggt gcgccacgtc gagtacgcca ccgtgggtga
    28801 cgaccagcac cttgcgcccc gggtgggtgg cggcgatgcg ttccagggcc gcacggatac
    28861 gcgcggcaaa tccattgagg ctctcgccgc cgccgggaaa gacgaagtcc ggatgacgcc
    28921 cttcgaagcg gtggtaatcg tcggggaagc gggcgcgggc ctcgtcgtag gtcagggcct
    28981 ggaagtgacc gtagtggcgc tcccgcaggg atgcgtcgtg ggtgatcgcg agggcgcagc
    29041 gctgggcgat ggcctcggcg gtctgacggg cacgcagcag gtcggagctg taggcggcgt
    29101 cgaagccctc ggccgcgagg ctggcggcgg tggcgcgggc ctgggcgagg ccggtgtcgt
    29161 tgagggggat gtcggtgtgg ccctggaggc gacgctcggc gttccaggcg gtctcgccat
    29221 ggcggaccag gcagatgcgg gtgatcatga ggctgacgcg ggaatgcggg agcgcaatta
    29281 tcgcactgcc gccagcgtcg gggccgatcc ggcgccggtc agtgaaatcc ctgattgcgc
    29341 cggggccgga ctgaccgata atccgccagc gcgtttccgc ccggaaatgg cattcatacc
    29401 caaaaacaca aggagacggg aaggtgcaag ccttgctcaa gcttagccgg gggatcgacg
    29461 ccctgacgga tcgggtcggc aggaccacga cctggctggt gctggtcgtg accctgatca
    29521 gcgcgatcaa cgcctttgtg cgttacgcgg tcaattacag ttccaacggt ctgctggaga
    29581 tccagtggta cctcttttcc gcgatcttcc tgttgtgcgc gggttatacg ctgatgcgca
    29641 acgagcacgt gcgcatcgac gtggtgaccg gtcacctgtc gccccgcgct catgcgtgga
    29701 tcgatatcgt cgggacgctg ttcttcctgg cgcccatggc cctgctggtg ctgtatctgt
    29761 cgtggccgat cttcgtcaat tccttcctca cccatgagca gtccagcaac gccggcggcc
    29821 tgacggtgtg gccggtgcgg ctgctggtgc cggtgggctt tgcgctgctg atcctccagg
    29881 cggcgtcgga attcatcaag cgggtggctt ttctgaaggg cctcatcgac gatcccagcg
    29941 cgaagcacaa gggcccatcc gccgaggaag ggctggccga ggcgatccgc cggcaacgcg
    30001 gagaggaggc ataaatggaa ttcctcgccg ccaacatggc gcccctgatg ttcgggtcgc
    30061 tggtgatctt cctgctgttc ggctatccgg tggcgtttgc cctggctgcc aacggcatcg
    30121 tcttcgggct catcggtatt gaactggggc tgctctcgcc ggccctgttt caggccctgc
    30181 cggaccggat cttcggggtg atgtccaatg acaccctgct ggccattccc ttcttcacct
    30241 tcatgggcct gatcctcgaa cgctccggca tggccgagga cctgctcgac accatcgggc
    30301 agctcttcgg gccggtgcgc ggcgggctgg cctttgcggt gatcttcgtg ggcgccatcc
    30361 tcgcggcgac caccggcgtg gtggccgcat cggtcatctc gatgggcctg atctccctgc
    30421 ccatcatgat gcgttacggc tacgaccagc gtctggcgac cggcgtgatc gcggcgtccg
    30481 gcacgctggc acagatcatc ccgccctcgc tggtgctcat catcatggcc gaccagctcg
    30541 gccgctccgt gggcgacatg taccaggggg cgatgatccc cgggctggtg ctgaccggct
    30601 tctatgtggc ctacgtggcg ctggtggcgg tgttccgccc ggccgcggcg cccgcattgc
    30661 cgcccgaggc gcgctcgctg cggggcatga ccctggcgat ccgcgtgctc acgaccctgg
    30721 tgccgcccct gatgctgatc ttcctggtcc tcggcacgat cttcctgggg gtcgccacgc
    30781 ccaccgaagg gggcgcgatg ggggcctttg gcgccctggt gatggccgtc ctgcggcggc
    30841 gcctcaacct gagccttctc aggcaggcca tggagaccac cgccaagctc actaccttcg
    30901 tggtgttcat cctggtgggc gcgcgggtct tctccctgac cttctacggc gtggaaggcc
    30961 acctgtgggt cgagcatctc ctgtccggtc tgccgggcgg gcggctgggc ttcctgatcg
    31021 tggtgaacat cctggtcttt ctgctggcct tctttctgga tttcttcgag ctgtccttca
    31081 ttgtcgtgcc gctgctcgcg ccggtggccg agaaactggg catcgacctg atctggttcg
    31141 gcgtgctgtt gggggtcaac atgcagactt ccttcatgca cccgcccttc ggctttgccc
    31201 tgttctacct gcgttcggtg gcgccgccca gcatcaagac cacccagatc tactggggcg
    31261 ccgtgccctt cgtgctgatc cagatcatta tggtgggtgt catcatcaca ttcccggatc
    31321 tggtgaccat gggcctcgac agccaggaaa aggtggacgc ggacgccatc gagatcgtcg
    31381 tgccgcaggg ggatgctccg gacgatgcgc cggtgcagtt tgacgcgggc aaggacgggg
    31441 acgcgcccca tgcaggtgac gggcaggctg gcgccggcga gcagaaggat ggcatcccgg
    31501 ttccgccggc cgatgatgac gcgaccaggg ctctggagaa agccctgcgg gaggaccgca
    31561 ggtaaggggc gtgcccgcca tgacccgcaa aacggaagcc cgacctgcaa gtcgggcttc
    31621 tgctttcggg aacgggatgc gtccgggcgg gtcggaaggg tggcaccggg atgtcctccg
    31681 gtcggtccct gcgccgcgtg accgggcagg gaccggtcct cggggctacc cggtgccggg
    31741 tgcagcagat gtctgcaacc gtccggcttt aacgggagtt tccatgaccg cggcagaccc
    31801 tgtcagcgta atgtcgatgc aaatgtaatt cacctggttt ttccttttgg gtgtgacaaa
    31861 agtcgtaggt gcccggtcca ggcggtcact gccgacgtcg cacgggtgga tgtgcagggg
    31921 ctggcgagcg ggtatattcc agcgtcgtcg cccggccgcc gtgttattcc cgatgcctgc
    31981 cgcctcgtcg gcggctgact gtgcccgtcc gttcggatca ggcaactgac gcgatctccc
    32041 caacatttcc cgcaggattc ttgccatggc cttgctgact tccctgtcac ccgatgtgtt
    32101 tcttgaacgg ctccgtgctg cccttgccga gccgcgaagg gatcatccgg agctggcgcg
    32161 gctggcgcgg cggctggcgg ccttcattgc cgcagaggat ctgccggacc gcctcgatgc
    32221 ctggcatgcc ctgatgacct ggttggctgg cgggcgactg tggcaggacg tctatatcga
    32281 tgtggtggat gtgcacggtc ccgccaccca gcgtctggcg gtgctgatgg acgtactggc
    32341 cggtgacgct gagctggccg gcgcactccg ggcggccctg cgcggattgc tcgacgagac
    32401 cgacggcgcc aaccttttcg gggaggtggg gattcccagt gaccggggct tcctgagtga
    32461 gttcggcgat cgcctgactg ccaagctgtt gccacagccg cgcgtcgatc atgatctcgc
    32521 gcatcttgtg ggccgcagct accggcgccg gcgggatctg gatctcgtcc gcgccctgca
    32581 tccgtccacc ctgcagcgtc tgatggagct cctttttccg gccgacggtc cggagatgac
    32641 ccgtgccctg cgacgcgcca tggcggacgg tttccgcctg ctgctggcgc gtgtggcggg
    32701 ccagggattg tcggagaagc tgcgggcccg gggcagcgcc caggcggtgg ccgacgtgcc
    32761 ctttgcgcgg ctcgcccgct ttggcgaagc gctggtggcc gactgggagg ggggcgccct
    32821 gcccgatgcg gccatggacg cctggcaggc gctgtcgcat gcctgccgtg ccgaaatgca
    32881 ggtcgtcagc cggcgcctgg agtctgaagg catcagcgtg gacatcgtgt acgggcttga
    32941 tgtgctcgaa cgctgcctga atcgtctgga tgccctggtc gcggtgatgg tggaaggcgg
    33001 tgccgcgccg gtgctggctc tggtccagca actggtgctg gcggcccacc agagcagcag
    33061 cctgcgccat ctggtgcgca tcaacctgca gcggctgcaa cggcgcatcg tggatcgggc
    33121 ggggcagacg ggggagcact acatcgccga tacgcccggt gagtaccgcc acatctggac
    33181 agccgcggcc gggggcggtc tgctcaccgt cggcacggca gcgatgaaga tgaccattgc
    33241 cggtgccggg ctggctctgt tcctggaagg gctgggctcc gggctcaact acgcggtgag
    33301 tttcctgctc ctgcaggcct tcgggctcat tctggccacc aagcagccgg ccatgaccgc
    33361 agcggccttt gcggccatca tccgcgaccg ccagggggcc gaacggctcg acagcatcgt
    33421 cgacgcggcc gcgcgtatct gccattccca gctggcagcg acaatcgcga atgtcgtggt
    33481 ggttgccttc ggggcctatg ccttcaacgg cctgtgggcc tggatcaccg gtcatgcctt
    33541 cctcacggcc agcgaggcac gctatgtgtt caaggccctc agtccggtgg atagcggcac
    33601 cgtgttctac gcggccctca ccggggtgat cctgtacctt gccgcgctgg tgggcgggtg
    33661 ggtggacaac tgggcggtct atcaccggct gcctgaggcc attgccgagc atcgcctggg
    33721 gcagcgggtc ggacggctgc gcatgctgcg tctggcgggc atcgtgtcgc gcaacatggc
    33781 gggatgggcg accaatgttt ctctgggcct gatgctcggc atgacaccgg tgctcggccg
    33841 gttcttcgga ctgcccctgg atgtgcgtca cgtgaccctc aactcgggca ttctcagtct
    33901 cgccgcggcc ggcatggacc tgcaatggat cggcaccggt cacctctcgc tggcgatcgc
    33961 cggggtggcg gtgatgttcg tcctgaatct gggggtgagc ttcgccctgt cgctgctcac
    34021 ggcgatccgc gcctacgaac tgcccgccga agacctgcgg gagctgcgcc gccgcctgtg
    34081 gcagcgtctg cgcacccggc cgcaagattt cctgatgccg cggcggttgc cgccagcgcc
    34141 ctgagcgtcc cgcctcaagc tcgctgccgc cgggccgata agcacccagc ggcggggcag
    34201 gccggcaccc agggggtgca ccggctccgc ctccggagac caccctggca accgcgggtt
    34261 gccaagcgag cgaagcatga atgcgccaga acgagttccc tttgccggtt cccgcgccgt
    34321 ggcgggcgag ctggcctgca tcgtccgggt caatgaagag atcaaggcgg tggtcgccac
    34381 ggccacgcgg atcaacctga tggccctcaa cgcaatcttc ctggccaagc gggcgggtaa
    34441 tgcggctctg ggatttggcg tcctgtccaa tgagctgcgc cggttcgccc aggatctgac
    34501 ccgccacatg gcggtgctga gagacatgac gtccggctcg gtcggcagcg tgacgggcgt
    34561 cgtccagcac cagcgcaact cgcgcatcct gtccgaggcg atccgcctga gcggtgaggg
    34621 tgtgccgggt gtggacatgg cccggcggcg ttgcaatcag cgcctggcgc gcgaactcgc
    34681 cagcttgcgt ggactcgacc agagactgcg cgcggcagtg gaggaaaccc agcagctcgt
    34741 cgaacttggc ggcgtgctgg cccgctcggc caagatcgag gcggcctacg gtggtggctt
    34801 cagcccggcg ctgatgcagg tgtcgacgga ttttgcggac gtgatcgccc agatcaagcg
    34861 ctccctggaa aacctcagca aggaacggct cgtcaaggac tgaaccctat gaaacaggcc
    34921 cactacatct atcaggacgg cgatcatcgc tggctggcca ttgcccgtga cccgaacaag
    34981 ccgggctacg tcatcgatac caacgaatac gtggtggccg acgaggaggc tgccctgctg
    35041 ctcgacccgg gtggcatcga aatcttccct gcggtgttct cggccctgtc gggcgaatgt
    35101 gatccgacgc gcctgatggg catcttcgcg tcccaccagg acccggacat catctcgtcc
    35161 ctggccctgt ggctggagtt caagccggac ctgaagtgct acacgagctg gctgtggtcg
    35221 tccttcctgc cccacttcgg cggagcgtcg gaaaccttcg tgtccattcc ggacggcggt
    35281 atgaccatca aggtgggcag cctggatctg caggcggttc ccgcacacta cctgcattcc
    35341 tcgggcaact tccatctcta cgactccaag gccaagatcc tcttttcggg ggatgtgggc
    35401 gcggccctgc tgccgcccga caacaacggc ctgtacgtgg aggacttttc gcgtcacatc
    35461 cgtcatgctg aagccttcca caagcgctgg atgggctccg accgggccaa gcgggattgg
    35521 tgcgagcggg tttcgcggct ggagatcgac atgctcgtgc cccagcacgg agccatttac
    35581 cagggcccgg atgtgcagcg cttcatcaac tggtttgccg cgctggaggt gggtgtgctg
    35641 cggccctgat gcgggcaggc cgcgcgccgg tctcaacgcc ccgtgcgccg gaaggtccgg
    35701 tggcggggcg tattgttctt gtcggacggc aaggtgtgcg ggtggtgact ttcgtagaat
    35761 gatccccgtc ctagcttccg tcacgccgtt acccgccggc ggtgtgtgcc atgtacctcg
    35821 ccatcaagca cctccatatc acctgcgtcg cgctcagcct ggcgggcttc ctgttgcgtg
    35881 gactatggat gtgcgccgac tcacccctgc tgtacgcgtc gctgacgcgg cgcctccccc
    35941 atatcgtcga tgcgctgttg ctgggcagcg ccatcgcgct ggccgtgatg agcggtcagg
    36001 cgccattcgt gcaggcgtgg ctgacggcca aggtcgcagg gctgctggtg tacatcgtgc
    36061 tgggcgcgct tgccctgcgt ccgggccgcc cgaagcccgt acggctgggg gcattcggcg
    36121 cggcgctggt gagcttctcg tatatcgtca gcgtcgcgct gaccaagaat ccggcagggt
    36181 ggctggcgat gtggggttga gcctcaggtc ggcccgtggt tgagcagcgc cttgagtccg
    36241 gcgatgtcga ggatgcggat gtgcttctgc tggaccgaca cgaatccttc gtcctgaaag
    36301 cgcgagaagg cccgcgagac ggtctcgagc ttgagcccga ggtaggaacc gatctcgtcg
    36361 cgggtcatgc gcagatggaa ctcggatggc gagaagcccc gtgccgtgaa gcgctgggac
    36421 atgttgagga gaaaggcggc aaggcgttcc tcggcgcgca tggagccgag cagcatcatg
    36481 acgccgtggt cgcgcacgat ctcgcggctc atgatcttgt gaaactggtg ctgcaggctt
    36541 tccacttctc gggagagctc ttcgaggcgc ccgaagggga tgacgcagac ctcgctgtct
    36601 tcgagcgcga tggcgttgca cgtgtggtgt tcgatgctga taccgtcaag ccccatgacc
    36661 tcgccggtca tctggaagcc ggtgacctgg tcgcgaccgt cttcgaggat cacgtcggtc
    36721 ttgaaggatc cggtccggat ggcgtagatc gcctcgaacg ggtcacccga gcggtagagg
    36781 tgctgatgac gcttgatttt ccgacggctc ccgacgagct cgtcgaggcg gtcgatctcg
    36841 ttcgggtcga gcccgaaggg caggcacagt tcccgcagat tgcattggga acacgcgatc
    36901 ttgacttccg tgacggacag cgggataacc ggtttcgtgt gcactcagat tcacctgaac
    36961 tttggggcct tgcggttggc gagcaacctt tgaagccctt tgatccacat caatacgatt
    37021 tccgggggat tctgggatcg ttccacgcac cctacaatgc gctcccatga acacgatcca
    37081 caaagacctg atcttcgatc cgcagctcat tcgcaagttt gacgtgaatg ggccgcggta
    37141 tacgtcctat cccaccgctg accgcttcgt tgaggccttc aactccgaag ccctgtgcga
    37201 ctggttgggc aagcggacga tcgggggggt gggaaagccg ctctcattat acttccacat
    37261 ccccttctgc aatacgatct gctactactg cgcctgcaac aaaatcatca ccaaggatca
    37321 cgggcgttcc gccaagtacc tgcgctatgt ggcccgcgag atggagatgc aggttgccca
    37381 tctcaccgga ccgcgcgaag tcctgcagct tcacctgggc ggcggtacgc ccaccttcct
    37441 ctcccacgac gagatgcggg agctgatggc caacgtccgc aagcacttca cgctggtgcc
    37501 ggacggggag ttctccatcg aggtcgatcc gcgcaaggtg gacgaagcca cggtggcatt
    37561 gctggccgag ctcggtttca atcgcatgag cgtcggcgtg caggatttca atcccgacgt
    37621 ccagcgcgcg gtgaaccggg tgcagagcga ggaggagacc cgtctggtca ttgaatccgc
    37681 ccgcgcccac gggttcaagt cggtcagcgt cgacctgatc tacggactgc ccaagcagaa
    37741 cgtgatcagc ttcaatcgca cgctggagca gatcctggcg ctctcgccgg accgcctttc
    37801 gatctacaac tatgcccatc tgcccgggct gttcaaaccc cagcggcgca tccacgacgc
    37861 cgaactgccc aatgcggagg ccaagctgca gattctgcaa ctggcgatcc gccgcctgac
    37921 cgaggccggc tacgtgtata tcggcatgga tcacttcgcc aagccggacg atgaactggc
    37981 cgtggcccag cgtcaggggc gtctgcatcg caacttccag ggctattcca cctacgcgga
    38041 atgcgatctg ctggccttcg gcgtctccgc catcggcaag gtcggaccga cctacgcgca
    38101 gaacgtcaag actctggacg agtactacga catcatcgac gcagggcgcc tgccggtcct
    38161 gcgcggcatc gagctgacgc ccgacgacct gctgcggcgc tccatcatcc aggcgctgat
    38221 gtgccacttc gagctgtcca tcgagtccat cgaaattgcc catctgatcg acttcccgcg
    38281 ctattttgcg gccgagctgg cggatctgcg cgagatggag aagaacggac tggtgcagat
    38341 cgacgataaa tggatcagcg tgcagcccgc cgggcgtatg ctggtgcggg caatcgccat
    38401 ggtgttcgac cgctatctgc gggccgaccg ggagcgcacc cgttactcca aggtgatctg
    38461 agcgtcgatg ggcgtctgcc cgatgcgggc tgatcgcgcg ctcccgcgtg gtgtagcctg
    38521 ttccgcggcg cacgctggaa tgtcccgacc tttcatgatg gaatgcgccc cgtgtctgcg
    38581 gggcgttttt catgcgcact tgagaatcca tgcctgaaac cggttatctc gccgtcttcc
    38641 tcatcggtct gctgggaggc acccactgcg tgggcatgtg tggtggcatc gtcagtgccc
    38701 tcaccgtgcg catgcccggg gagcagcggc gtgactggcc tctccacgtg gcttacaact
    38761 tcgggcgcat cgccagctac accgcggccg gtgcggcgct gggcgccctg ggcagcgcgg
    38821 gcatgctcct cgacggcatg ctgccggtgc agctgggcct ctatgtggct gcaaatctga
    38881 tgcttgtcgc cctggggctc tacctgacgg gcttcacccg cgcactggcc ggcgtcgaac
    38941 ggatcgggca acgcctatgg acgcgcatcc agcccctgac gcggcgcttc ctgccggcgc
    39001 gctcggttgc gcaggccctg cccctgggga tgctgtgggg tttcctgccc tgtggcatgg
    39061 tctatagcgt gctggccacg gcgctggtca ccggatcagc ggggcggggg gcgctgctga
    39121 tgctgtcctt tgggcttgga acgctgccca acctgctcct cgccgggctg ctcctgcgcc
    39181 atctgcgcga ggtggtgcgg aaccggtcgg tccgcctggt gtccggactg gtggtgctgg
    39241 gcttcggtgt gtatggcctg ctcaatacgt cgagcctggg caacaagctg tggaatggcg
    39301 tggtgtgcca tgtgtgaggg ctgcggcccc gagacaaggt gaaggcaatg aagatcctgt
    39361 tggcggtgga tggatcgccg gtgtccctgc gggcggcgga ggaagtggtg cgcctggcgc
    39421 gggcactgaa atccgagccg gacgtccacg tggtgtatgt tcatctgccc atcccgctgg
    39481 actttgcaac ccgtcatgtg ggccgtgagg ccgttgaggc ctactaccgg gacgagggtg
    39541 aggcggtatt gcaggcgccc ctggctgcat tgacggcctc cgacctcatg ccgcaacgtc
    39601 acctgcacgt ggggcagccg gccgatgtga ttctgcaact ggcgggcgaa ctgggctgcg
    39661 atctgatctg cctcgggcgt cacgggcgcg gcgccatggc cgaccttctg ctgggctccg
    39721 tggccaaccg ggttgttcag cgcgcaccga tgccggtact cctggtgggc tgagagcaga
    39781 tcatgtcgga cggcccgttg cccgaatcca tggatgagct gtccatcacg ggcatgagtt
    39841 gcgccgcctg cgcggtgcgg ctcgagaagg tgctcagccg cctgcccggc gtgtcggcgc
    39901 acgtgaattt tgccaccggc cgggcgagcg tccggcgtgc gaccggcgcg gcggacggcg
    39961 aagcccttgt cgctgcggta cgcaaggcgg gtttcgacgt ggcgcccgag accctcgagc
    40021 tggcactctc cggcatgagc tgcgcggcct gtgcggcccg gatcgagaag gtgctcaacc
    40081 gcctgccggg tgtggaggcc acggtcaatt tcaatgcaga gacggcgcgc gtccgctatg
    40141 tgcccggtct ggcagggccc gacgccatgc gggccgccgt cgccaaggcc ggattctccg
    40201 ccgagcccgt tcttgcccgc gaccgggagg cggagcgcgc ccgcaaggcc gaggaggcgc
    40261 gaagcgaatg gcgtcagttt ctgctggcac tggtcctcag cgcgcccttc ctgctccaga
    40321 tgcccttcat gctggcgggt ggcctggacg gccatcacga atggctgcct cgctggctgc
    40381 aaggtctgct ggcggcgccg gtgcagttct gggtcgggcg gcgcttctac cggggggcgt
    40441 ggtcgtccct gcggggtggc ggcgccaaca tggatgtgct ggtggcgctg gggaccagcg
    40501 tcgcgttctt cttcagtctg gccgtggtgc tccttgggcg gcacgatcag catgtgtatt
    40561 tcgaagcctc ggccagcatc atcaccctgg tgctgctcgg caaactgctg gaggctcggg
    40621 cgcgggcgcg gacctcggcg gccatcgagg ccttgctgcg tctgcagccg cccaccgcgt
    40681 ggctggagcg ggacggcgcg ctggtggaag tgcctgtcgc ccaggtggcg cggggcaccg
    40741 tgttcgtgct gcgggcgggc gacgcggtgc cggtggacgg ggaggtgctg gcgggcgagt
    40801 catccatcga cgaggcgatg ctgaccggcg agagcgtgcc ggtgcccaag gcggcggggg
    40861 acacggtctt cgcggccaca ctcaacggcg ccggcctgtt gcgctgccgg gctaccggcg
    40921 tgggtgcgga gacgctgctg gcggggatcg tgcggctggt ggagcaggcc cagggcggca
    40981 agccggccgt gcagcggctg gctgaccgga tcgcggcggt ctttgtgccg acggttgtcg
    41041 gcctggcgct gctcacattt gccgcctggt ggtgggcggc cggggatccc gtgcaggcgt
    41101 tggtgaatgg tgtggcggtg ctggtcatcg cctgcccctg cgcgttgggg ctggcgacac
    41161 cgacggccat catggtgggt acggggcagg gcgcccgcgc cggcatcctc gtgcgcaagg
    41221 tggaggcgct ggaactggcc gaatccctgc gcatcgtggc ggtggacaag accggcacgc
    41281 tcaccgaggg gcgtccggcc cttgtggaga cccgcctcgc caagggcatc gcctacaacg
    41341 aggccctgca cctggccgcc agcctggagc aaggcagccc tcacccgctg gcggtggcac
    41401 tggtggcggc cgcgcgggag gcgggcatcg ggctcgccct gccggttgcg gtgcagtcgg
    41461 cgggtggcaa gggtgtgtcg ggcgaggtgg cggggcggca ggtggcggtc ggctcgcctg
    41521 cctacctgac ggaggcgggc gtggcgattc cggcggatca gctcgatccg ctgactacgg
    41581 ggggattgac gcccgtctgc gtagcggctg acggccgcct cctggcgctt ttcggggtgg
    41641 ctgaccggct gcggccgggc gcggcagagg ctgtggcgcg tctgaggcga caggggctgc
    41701 gggtcgtgat gctgaccggc gatcatcccg cggtagccca agcaatcgcc cggcaggcgg
    41761 gcatcgacga atttcgcgcc caggtgctgc ccgctgacaa ggcggcggcg gtgcaggccc
    41821 tgcgcaacga aggacgggtc ggcatggtgg gggacggcat caacgacgcg ccggccctgg
    41881 cggcggcgga cgtgtccttc gccatcggcg ccggatcgga cgtggcggtg gaggcggctg
    41941 acgtgacctt ggtgcgcagc gacccggccg gcgtggcaga cgccatcgcc ttgtctcgcg
    42001 caacgctggg caagatccgc cagaatctct tcttcgcctt catctacaac atcctgggca
    42061 tcccggcggc ggcgctgggg ttgctcaatc cggtcatcgc cgcggcggcg atggccatga
    42121 gttcggtgtc ggtggtgagc aattccctgc tgctgcggcg ctggcggccg gggcaggact
    42181 gatttcagga ggacaggacc atgcaggaag cgatcattca ggtgggcggc atgagctgcc
    42241 agggctgcgt caggaacgtg accggcgcac tgtcggcggt ggctggcgtg acggccgtcg
    42301 aggtgtcgct ggagcggggc gaggcgcggg tgaatttcga tcctgcccgc acgggcgtcg
    42361 ccgccttgcg ggcggcggtc gaagaggcgg gtttcgacgc ggcctgaggg cacggcggcc
    42421 gcatcgtgtg caccgcgtgc gcggccggtt tcacatataa tcccgcggat taattcaaac
    42481 ggaggtaggt atgggttacg accaggtcaa ggcaggcaag gacgttccga acgacatcaa
    42541 cgtcatcatc gagatttccg cccagggcga tccgatcaag ttcgaggtgg acaaggacag
    42601 cggcgcggtt ttcgtggatc gcttcatggg caccaccatg cgctacccga tcaactatgg
    42661 ctacgtgccc cacaccgtcg ccggcgacgg tgacccggtg gacgtgctgg tcgtcacacc
    42721 cttcccgctg cagccgggtg tcgtcatccg tgcccgtccc gtgggcgtgc tgaagatgga
    42781 agacgaaggc ggcgtggacg ccaaggtcgt cgccgtgccc gtctccaagc tgaccccgct
    42841 gtacgacaag gtccagtccg tcgaagacct gcccgaactg ctgatgaagc agaccgtgca
    42901 cttcttcgag cactacaagg atctggagcc cggcaagtgg gtcaaggtta ccggctgggg
    42961 caccgtcgag gacgcccgcc aggagatcct gaacggcatc gccgcggccg ccaagtaaac
    43021 gtccggacct ccacggcttg aaaggcccgc gcaagcgggc ctttgtgttt atgcgtgacg
    43081 cattacgtca acggggtgac gattttcgtc acccggacag cgcttgctgg gcctctagac
    43141 tgccgtagag ggggagggtt ttttgttagt cgtatgtttt gtaaggattt attgtatttt
    43201 ttcatattct ggcatgtgct ttgcgttgct tttcttgccg ggcggacttg cccgttcaat
    43261 tcgaacatca tcctctggag ctgagaatca tgaccaagca aaagcaaagc ggctttaccc
    43321 tgatcgaact gatgatcgtc gtggcgatca tcggcatcct ggcggccatc gccgtgccgc
    43381 agtaccagag ctacaccaag aaggccaagt tctccgaagt ggtgaatgcc acctcttccc
    43441 gcaagtcgtt tgtggaagtg tgtctgcagg atccgaatct ggtgcaggct aactgcaatg
    43501 gcggtgccaa tggtattccc gcggacgatg ctgttggatt cggcaacgtg accagcattt
    43561 ctgttgcatc gggtgcaatc acggcaattg gtaagtcggg tgtattcggc agcggcgcca
    43621 cctactcgct taacccgacg gtcaccagcg gcaatgttgt gtggagtgac accagcagtg
    43681 catgcaaagc ggcgggtctc tgctgatctt tctgcgaacg taaaaaatgg accgcattgc
    43741 ggtccatttt ttttggtacg tattggttta gtttttgcgt gtttcatgat ttttgatgat
    43801 gactgattct gatgtcaggc gcaaatttag ctcgccaggc atggtgagtt ttcttcgcca
    43861 tactcaactc gctggaccct ttgttgtctg gattttgccg gcatgcttcg cgcttgcaag
    43921 ccttgccatt gtttttggtc agattgatga tctgttaagc cccttcgcag tgtggtccac
    43981 accgggatcg cgagcgatgg cgtatttcat cgttggtact ttggtcttgc ccgtctatgc
    44041 atttacccga ccgcctgtca aggagttggc tcttgtgctg gggggggggc tcttcagtgg
    44101 ttggtttatt ggtgttagtg cagagcaatt attttcatct ggagtaacgg gatttatagc
    44161 gccgttcgga tttccttttt tttgtatggc ggtcgttgct ctggtcggac tgactgcgcg
    44221 cgcgctgctg acgcggaata ctattgcagt catgtcagat ccacgcgtaa gcgcattgat
    44281 ggcctgcatg tttgcattgc tgtatatgtt tttttcgcca acgttcgtta acgtgacgat
    44341 catttttcat ccagagactt tcgataatcg cctgtatgca ttggatgcgc tttttgggtt
    44401 tgaggcgagt gtcttcgtcg gcaagctttt cgtgacgcac tcggcggttt tgacattctt
    44461 tcaattggct tacgcgttca tgtttgtaag cttcttggcc gtgctgggct ctcagatccg
    44521 tgaagaatgg cgtggccagg gctggcaggt cgctgaggtg tggtttgtga tcagtatcct
    44581 cggggtgatg gcctaccatt tatttccggt aagcggcccg gtctatgcct ttaaagacaa
    44641 gtttcccgct tcgatgcccg atccgtcggg cgttggtctc gtgtctgcga tcgtggcgcc
    44701 agccatgcgt aacggcgtgc cgtctttaca cctgggctgg agcttggccg cgtggttggt
    44761 gtccctgccg ctacggaatc gaactatcaa atgggtgagc ggtctggtgg ccctggccat
    44821 ggtgccggcc acacttggac tcggcgagca ctatttgctt gatctggtga ttgccgttcc
    44881 gtttgtggtc gggacgctgg cattgactgt ccgtgggccg ggttggcagt ctccagccag
    44941 gcgtatggct gcatggacgg gctggggatt cttttttgtg tggctgattc tgttacgttt
    45001 cgcggcggaa tggttgctgc aggcaccctg ggtggtgccg gtgctttcga tcgtttcgtc
    45061 gggtgcgtcg gcctggcgga tgagggcgtt gatatgcgcc gtgcgcgggg ggggcgaagt
    45121 cgcggaagtc acacgcgtag atcaggcttc ggcgctcggc gggtctttca gtgtgcgtct
    45181 ggcagcgctg ttttttctgt ccggctttgc ggcactgatg tacgaggtgc tgttttccaa
    45241 gaagctcgcg gtgacgttcg ggagttcgtc gctggcaacc tataccgtgc tggctaccta
    45301 catgggtggc atggcgatcg gctcttggct gggggggatc ctggctgccc gcgggggaga
    45361 tttgctgaag cgctacgtgg tgtgcgaggg gagcatcgcg gcgtattgtg tgctgacgcc
    45421 cttcttgttt gacgcagttc acgatctgta tgcccagtgg gcaaccaatg cccccgtcga
    45481 cgccgggtgg gtggtggcct tgcgattcgg gctgggtgct ctggtcctga tggtgccgac
    45541 gatcctgatg ggcatgacgc tgcccatcct gacggcctgt ctcgaccggg gcgcggcggc
    45601 ctatggacgt gtcgtgtccg tgctttacgg cgccaatacc ctgggcgccg cgctgggggc
    45661 gttgctcgcg ggctatgtgc tgatcccgac cctgggcctg caatcgacca ccctccttgc
    45721 tgcgctgttg aatcttcttg tcgcggcttt tggcggggag atgggcaaac gactgaagca
    45781 ggtgggggcc cgtctggagc aggacgaaga tgccacatcc gctctgtccg ccagcggccg
    45841 cgccttgccg gggtggctgg tcctgatgag cttcggaggc gtgggcctgg tatgcctggg
    45901 gcttgagacg ctgtacatgc acctgctggc ggtggtggct gggaacagta cctacgcgtt
    45961 ctctctgatg ctgtttgcct tcctgctggg gttgggcggt ggggcaacgg tcattcggca
    46021 gattccgcag gcaatcagcc ggccggaagt ggccatggtg gtattgctga tgctgttggc
    46081 tatgtgtctg ggcatcgggg aaagccaatg gagcaccatg gcggactact tcgcgtcatt
    46141 tgcggggtat ccgatgccga gcagctttgg tgggcgggag ctggtgagag cggctgtatg
    46201 cctgatcgcg ctggttccgc cggcgttcct catcggggcc tttttcccgc tcgccatggg
    46261 ggccgtcgga cgtgccggtg gcgtgcgggc gcttgggact ggtgcagccg tcaatacgct
    46321 gggcaatatc gccggggtct tcatcggtgg cttcgtgctg ctgccgacgg ttggtgcgtt
    46381 gggtggcatc aaggtgctgg cggtcctcgc aatggggctt gccgtgtgca tcgtcatcgg
    46441 ggcgggcctg atgcgggagc gggtacttgt tgcactgggg gggatggccg cgctctgtgt
    46501 cgttgcggcg ccgacggatc tggactacac gcggctcgcg tcgggagcca atgtctattt
    46561 ccagcctcag cactgggggc gggtcattga tcacgcggag agcgcggacg gcgggttgac
    46621 atcggttcat gtgacgctgc aggacggcgg catgaccatg aagacgctga cgacaaatgg
    46681 aaaattccag ggcaatgatt ccggcgaagt ggaggcacag atcgggtttg ccctggttcc
    46741 gctggccgag aatccccgca ggaatgatgc gctggtgatc ggctacggta ccgggacgac
    46801 cgcgaatacc ctgaagcacg cgggcttccg tcagattgac gtggtggatc tgagtcggga
    46861 tgtgatccgt ctcgccaacg cccatttcgc ccatctgaac gagcgtgtga cggaggcgcc
    46921 aggggtgaag ctgcacattg cggatggccg caattatctg ttgctccagc atcggcagta
    46981 cgacctgatt acggtggagc tcacctcgat ctggtttgcc ggtgctgcgg ccagctacaa
    47041 ccgcgagttt taccagttgg cgcgtcagcg tctgcgccct ggcggcgtcc tgcagcagtg
    47101 ggtgcagttg catcatttgt ccggacagga tctgctcgcg ttgctggcat cggtgcgttc
    47161 cgaatttgcg gacgtgaagg tcttccattt tggcgggcag ggcatgatcg ttgcccgcaa
    47221 tggggggggc gcgcccatgg ccgttggcat gggacggcca caggatgaac cggcgatccg
    47281 ggcctacagc gaacgcattc tgggccgtcc gctggcgaag ggacctgaga ggctgctcga
    47341 tgcccagggt gtcaataact tcctgtccca ttggggcgaa gcggaagact gggcgtctac
    47401 cgatgacaac atggtcctcg agtacaacac gcccannnnn nnnnnnnnnn nnnnnnnnnn
    47461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47521 nnnnnnnnnn nnnnnttcgg gaaggcgccg gtcaagcgtc aggatgagca ggaaggggga
    47581 cggcattgat ttcccccaac tgcgataccc gtccagccga ctgccccgtt gtattgatcg
    47641 tacttcacgc catcagtctg ccgccggatc ggttcgcagg cgacggcgtg gagcggttgt
    47701 tcaccaatcg tcttgattcc gccgggcatc cctattacgc ggcgatccat catctgcggg
    47761 tgtcggccca ttacttcatt cgccgcacgg gtgagcagat ctgcttcgtt gcgccagcgt
    47821 tgcgggcctg gcacgcgggg gtgtcgagct ggcgggggcg ggagcggtgc aatgatttct
    47881 ctatcggaat cgaacttgag gggtgcgaca gcctgccctt tgagccgctt cagtacgagg
    47941 tgctggccga cctgctcgcg acgctgtgcg agcagtttcc gatcgagggg atcacggggc
    48001 atgcggacat tgcgccgggc cgcaagaccg accctggtcc gtacttctcg tgggagcgct
    48061 tgcggcggct tctgaccgtt cggggcgggg ctgcgctggc ggcgcgggtg gagccggaat
    48121 ccgccctcga cgagtccgtg cagatgcgct ggccgttccc gtcaaacagg cgtccggtct
    48181 gaattcatcg gggtgagcgg gtgcccggaa tgcgggatga ggcgccatct gaccacgctc
    48241 cgagagaccc cggattatgc aagcgagatt gcatgtcgat ggcctcgggc gcggggcgat
    48301 tgacaaaacc ctgttgaatc aatacaatgc aaattgtgtg cagtgcagca cgcataaaag
    48361 aaatttaacg cccgtgccgg ggcagcgaat ttcagcgggt ttggcccgct tcgctgttgt
    48421 tgttccgaca ctccccatgt cggattccgg tacgtttttg gcacgctgtc tggttgcatc
    48481 agcgtgtttg gtcaaccact ggtgaccggg cttttcagag cgccgccggc cgttccaggc
    48541 cggtgagtcg aatcaggctc cgttgttcag gagccctggt gaagcagagc ggactcctct
    48601 tcctgtctta gggcaggcgt gggtgtgcgt gtgcgttcga aagggacggc cgcaagaaag
    48661 gagaacccat gttcgcaact cgtatcgtga agtacgcacg tcacgcggct tcattcacgc
    48721 ttcacttcac ccacggcggg ttgctggtcg ccggtcttat cgtcacactc ttcgtcgcct
    48781 cccggattgc cactcatggt gtgcagggtc ttgcgcttgg ttccctgctg gggagttctc
    48841 ccgtggtagc ggcacaggcg ccggacagcg aggtgcagga ccaaacggag gaggtgggcc
    48901 aggaaatcgc cctgagcccg gacctgcagc gggtcaagag ctatctggcc aaacgctacc
    48961 aggtgtcggc ggtggcgatc gagccgcttc tggcgtcggc gcagcagacg gggcgcagcg
    49021 tgggtgtcga tcccttgctg ctggtggcgg tgatggccat cgaatcgcgt ttcaatccgt
    49081 ttgcggaaag cccggtgggt gctcagggac tgatgcaggt gattccccgc ttccacacgg
    49141 acaaggtgga tgtcaaatct gacaaggccg ccctgttcga tccggacacg aatatccggg
    49201 tcggagcgct ggtactgaag gagtacatcc gcaacacggg ttcggttgag cgggcgttgg
    49261 agcagtacgg tggctccagc gatccggcta cgccctacgc catcaaggtc atggcggtga
    49321 aggcccagct tgcggccgca gcccggggca agatcatcgg cgcctgatcc tttttag
//
LOCUS       DAOQPA010000009        44282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_332_length_44282_cov_20.134578, whole genome shotgun sequence.
ACCESSION   DAOQPA010000009 DAOQPA010000000
VERSION     DAOQPA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 44282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44282
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_44282_cov_20.134578"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKX01_02510"
     CDS             complement(<1..704)
                     /locus_tag="PKX01_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75023.1"
                     /translation="MHVLSRRGRVAGWVVAGMLVASAAMAGEHGTCQPVPALEFGPYD
                     YRVAPPDKLLRVENYHFDTGVESLTKGKSSAYVGHDLEFTLRYFPNHFRALNAMVKLG
                     LKQKTPTPTGTTETVDCWVSRAIQFRPDDGLMYLINGLWLVQRGMKAEAAEQMAKARE
                     LQVDSANYQYNLGLGLLMVDKPQQALEAAHKAYALGHPLPGLRNKLQRIGAWRPQPEP
                     APEDPPGTAAPPANTGA"
     gene            complement(759..1757)
                     /locus_tag="PKX01_02515"
     CDS             complement(759..1757)
                     /locus_tag="PKX01_02515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ75024.1"
                     /translation="MGLLPRSLALKAEGLASLLPGKGRRLSVLIFHRVVPEPDPMNPD
                     EIDADRFDRLLTMLGREFHVLPMAEALSRRAAGTLPARSVAITFDDGYADNFTVACPI
                     LRRHGMEATFFVASGFLDGGLMWNDAVIETLRQYGGATLDLAWLDMGMCATQSVVQRQ
                     VLAEALLLKLKYLDLDGRKAALARLVAQTGSRLPEDLMMSSAQLRGLRDAGMEIGGHT
                     VSHPILAVLDEDEARSQMARDRARLAELLGRPPRFFAYPNGRPGKDYTAVHARLAREL
                     GYEAAFTTSHGSVGSESGLYELPRFTPWDHNLRKFSLRLLRNYLNVDAPQLASARA"
     gene            complement(1779..2768)
                     /locus_tag="PKX01_02520"
     CDS             complement(1779..2768)
                     /locus_tag="PKX01_02520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ75025.1"
                     /translation="MRGAFYRLARLLGLFTLASWLYRGRQSILCYHAFAFEDEHLFRP
                     QLFMQPATFARRLDLLARRGYRVITLDEALRRLDAGSARLRDVVITIDDGFISVLRHA
                     APALAARRIPAMLYLTTHYQTAARPVADLVAQYLIWRALRRHPERADALAALLPPGEG
                     GPIARYQRLDKPARRAFLRNARAAGVLEPESAGLDERFRIVTAEQVRALQAAGLSIEL
                     HTHRHRYPADPAQLRREIDDNRAALAACGVAAARHLCYPSGEWRAASFPVLEAAGVVS
                     ATTCIAGLNERTTPRLALHRFLDADDISDIEFEAEISGFKELLRGARRRLRGA"
     gene            complement(2770..4065)
                     /locus_tag="PKX01_02525"
     CDS             complement(2770..4065)
                     /locus_tag="PKX01_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HMZ75026.1"
                     /translation="MRDVALTLIMLVLVPSIFFRPSLGPLAWAWVSMMVPHRLTFGFA
                     YSLPFAQLVGGATLIALFAAKERHRIPWITPIRYLAYFYAVMCFTTIIALNNVGDAFD
                     MWVKVTKVHVMLWVTIALIGGKKQILDLIKVITLSIGFYGIKGGLFTIRSGGGERVWG
                     PTGSFFEGNNELALALVTIMPLMYFMRQTAERAWWRTVLSISLILVAFSVLGSQSRGA
                     LLAISAMALILGARSKRPVLAVSLFAIIGIGLAAFMPDTWSNRMNTISSHEDHSAQSR
                     LYTWQMILNMVAHNPWGAGFDFWTWEVWGRYAVTEWLLPYSPHSIYFQALGEHGILGF
                     FFYIGMGVTSWRFCGRMIKEVGNDPEQQWLKLLAQCIQISLLSFAVGGTFLGLVNFDV
                     PYYLVAIVGLLWRDCRKVPDAVTAPGAPAVPRPRPAGGL"
     gene            complement(4137..5486)
                     /locus_tag="PKX01_02530"
     CDS             complement(4137..5486)
                     /locus_tag="PKX01_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ75027.1"
                     /translation="MGLYTRVCSGVLFPLHERLKKHDSVARRKDLEITQRLPRAEIEA
                     IQARRLKAFLEDIGRHVPYYHTLFREHGFDPAGVTGAADLRKLPFMTKAVIRAHVDEL
                     KADDAGPLSRYNTGGSSGEPLIFYIGKARKSHDVAAKWRATRWWDVDIGDPEIVLWGS
                     PIELGAQDRVKAVRDSLLRTWLLPAFEMSDAKVRDMLAMIRAKRPRMLFGYPSALAHL
                     AERARLWKIPMDDLGIRVAFVTSEMLYAHQKQSITEIFGCPVANGYGARDAGFIAHQC
                     PHGHLHLSAEDIVVETIDPEGRPVAPGAPGEIVVTHTATRDFPFVRYRTGDIGVLSTE
                     TCGCGVNLPILAEVQGRTTDFVVGADGTVMHALALIYTVRDLPGVQQFKIVQHDIGRT
                     EVLVVADDRFGEAERQRIRHDYAARLGAGVQVDITPVSEIPPEKSGKTRYVVSHVKR"
     gene            complement(5499..6593)
                     /locus_tag="PKX01_02535"
     CDS             complement(5499..6593)
                     /locus_tag="PKX01_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ75028.1"
                     /translation="MEQPTYSGLRLAVVGPLPPPSGGMANQCQQLLGLLRAEGAEVEL
                     VRTNAPYQPEWAGSIPVLRAFFRLLPYLWALWRASGRCQVMHVLANSGWAWHLFAAPA
                     LWIARLRGAPVIVNYRGGEAEPFFAAAPRHVHRTLAAADALVVPSAFLDGVFRRFGFA
                     SEIVPNIINLERFGVPRTRTADDAPHVVVTRNLEPIYDIPSVLAAFAAVRAALPAARL
                     TVAGSGPELTRLQAMAADLGIAEAVTFAGRIDNAQIPALYAAADVMLNPSTVDNMPIS
                     ILEAFASGVPVVSTDVGGVPFIAEDGRTALLVPARDPARMAEAMLRILRDRPLAERLA
                     AAARDEVKQYAWPVVRQKWLAVYRRLARNR"
     gene            complement(6593..7612)
                     /locus_tag="PKX01_02540"
     CDS             complement(6593..7612)
                     /locus_tag="PKX01_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMZ75029.1"
                     /translation="MTALRRRVAGRAMPRLLLATVVCMAILAAFLGVQAIPSTEAVRL
                     RNALLVEPSSPADFTWTPAQVPAGFLQDAGPVEPLFADVVRNLGIAALPDDRAKALAL
                     SAHLSQHADQEGPIQSDLQTTYHAIRKGAGYCADYTAVYLALARAAGLFAREWGFSFD
                     GFGGHGHAVVEVFDRQHQRWMFLDVFNNFHPVYADSGEILSVAEFRAFLHGEGPAVRI
                     VKTGPGRAGMKYEDKLLAYYRHGMDQWYLWWGNAVFDYERAPVVAALGHVSRSLEQLG
                     AIVAGVHPHVRIVETPSNQALVARMTRLATQIRVIAAVEAVLALLMLVQILAWRRSGR
                     RENPL"
     gene            complement(7609..8925)
                     /locus_tag="PKX01_02545"
     CDS             complement(7609..8925)
                     /locus_tag="PKX01_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ75030.1"
                     /translation="MKISIFGLGYVGAVSLACLARDGHDVIGVDIDATKLDLIRQGKT
                     PVVEEGMVDLMKDVVAAGRVTVTTDAVHAVLNSDISLVCVGTPSAANGSQDQGAVLRL
                     AESMGVAIREKAAPHVVVFRSTLVPGTVEDVLRPIIENGSGKKDGEGFHLCFQPEFLR
                     EGSSIRDYDKPPFTVVGANHDYPAERLKALFGHLPCNFLKTSVRSAEMMKYCCNNFHA
                     LKITFANETARLCAALGVDPFEVMDLVCQDTQLNISRAYLKPGYAFGGSCLPKDLRAT
                     TYLAKRHDVEIPMLAGIMQSNRNHVDVAVNKVLETGKRKVGFVGLSFKTGTDDLRESP
                     LVSLAEQLLGKGVQLSIYDPEVHLSRLLGANKSFIERHVPHIGELLKPELADVVAEAE
                     VLVVGLSSKEVFADLARLARPGQKIIDLVNLPNVADIQADVEGLCW"
     gene            complement(8922..10136)
                     /locus_tag="PKX01_02550"
     CDS             complement(8922..10136)
                     /locus_tag="PKX01_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HMZ75031.1"
                     /translation="MNILYVCHRFPFPPKRGGKIRPFNMIRHLTAQGHAVTVCSLARS
                     PQEAEEGRGIAPHCKAFEMGLVSNPVQALRMVARLPVSTPSSMGFFYSPDLKAKIDRL
                     LASQKWDLIFVHCSSVAQYVEHVTDVPKIIDYGDMDSQKWLEYAHYKPFPLSLGYRLE
                     GLKMLAAEKRLAGKFDLGTATTRAEWETLDSYGTGLATDWFPNGVDAGFFCPDDTVYD
                     PDTISFIGRMDYYPNQECMTRFCQDIWPRVKAARPQMKLLIVGADPSPAMRKLGELDG
                     VTVTGSVPDVRPYVRSSALMVAPLAIARGTQNKILEAMAMGVPVVTSSIAAGGVDAEA
                     EKHFLVADTPEAYAAAIARITSDPVERRRLAEAGRARMLSHHAWPASMARLDRIIGRC
                     ITQFQESKEKRT"
     gene            complement(10133..12058)
                     /gene="asnB"
                     /locus_tag="PKX01_02555"
     CDS             complement(10133..12058)
                     /gene="asnB"
                     /locus_tag="PKX01_02555"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMZ75032.1"
                     /translation="MCGIHGFFRFDGQRVEPAQLTAMGDRTQHRGPDDEGQYIDGPCG
                     IAMRRLSIIDLAGGHQPISNADDSLWIVCNGEIYNFRELRAELQGLGFHFKTGSDSEV
                     VLHAYDAFGDDFVHRLNGMFDFALWDRRRKRLLIGRDRIGVKPLYVMQDARRLAFATE
                     AKALLALPGVSPELDRSQLASYLHLGYAAAPGSMFKGIRKLPPATLLSIENNEVREWR
                     YWRLPSVVERTVSEADWLERVRVRLEESVRMQMVSDVPIGAFLSGGVDSSAVVGYMAR
                     HSDRPIRTYAIGFEGGEAEALYNELPYARQVSQLFGTEHHEIVVKPDVVSLLPKLLWH
                     MDEPLADTAFITTFLVSEFARQDVKVILSGVGGDELFGGYRRYLGGHYAERFQRLPGF
                     VRSAATAVANRLPSDRHSGLLNTLRLAKGFIASANMAPDDRYRSYLQVLARETVSAML
                     LDAGAQAPDPLAQAFSDAGQADDLNRMFAVDAETQLPDDLLLLTDKMSMAVSLECRVP
                     LLDHELVELAAAIPAAVKIRGGRLKHLMKDALADLLPADILDRKKRGFGTPMGAWLKR
                     DLAPVLRQLLSPESVRRRGLFRHDVIAGLMADHESNRSDGTDGLLSLMNLEIWSRIYL
                     DGRSADDVAAELKSYTR"
     gene            complement(12068..13273)
                     /locus_tag="PKX01_02560"
     CDS             complement(12068..13273)
                     /locus_tag="PKX01_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HMZ75033.1"
                     /translation="MRVLHVLDHSLPLHSGYAFRTVSILREQRKLGWETFHVTTPKQG
                     VSSGGAEEADGWTFHRTANTGQEGLVAQMRLTAARLDDLVQTLRPDILHPHSPVLNAL
                     PALWVGRKHRIPVVYEMRASWEDAAVDHGTTAEGSLRYRVSRGLESFALRHADQVTTI
                     CEGLRGDIAVRGVPDERITVIPNAVDVSAFTFGAEADPELRRSLGLDGKTVLGFAGSF
                     YGYEGLDLLIRAARQLVDRYPDLRVLLVGGGPQDANLKALVAELGLSDRVIFSGRVPH
                     AVVQRYYELIDVLAYPRLPIRLTELVTPLKPLEAMAQGRMFVASDVGGHKELIRHGET
                     GFLFRAGDVDALAAAIDDVLTHRERWPQVRQQARSFVEVERTWATSVARYRDVYRRAL
                     TARGRTLSI"
     gene            complement(13276..15201)
                     /locus_tag="PKX01_02565"
     CDS             complement(13276..15201)
                     /locus_tag="PKX01_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HMZ75034.1"
                     /translation="MCGIAGIFDTRGQQDYPRELMQRMNDIQSHRGPDESGYHFEPGV
                     AFAHRRLSIIDLSTGQQPLFNEDGSVVVVFNGEIYNYQELIPELTALGHRFHTRSDTE
                     VIVHAWESWGEACVQRFRGMFAFALWDRNRQTFFMARDRMGVKPMYYAILRDGTLVFG
                     SELKVLMQHPDLDRTMDPLAVEEYFALGYVAEPRTIFKGARKLDPGFTLTIRRGEPIP
                     QPKPYWDVRFTLDHNLNLADATAELKERLKESVRLRMIAEVPLGAFLSGGVDSSAVVA
                     TMAGLSDEPVNTCSIAFDDPAFNESEFAQMVADRYKTNHRVETVQSDDFDLIDTLAAL
                     YDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDENMGGYRRYRFHMMEEKMRGGLPY
                     GLRRPLFGFLGSVYPKADWAPRVFRAKTTFQALARDSVQAYFHSVSILRDDMRRQLFS
                     QRFRQELGGYNALEVFRRHAANAQTDDPLALIQYLDIKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHPLVEWLASLPSSLKIKGQEGKYVFKKSMEPMLPRDVLYRPKMGFAVPLS
                     RWFRGPLKERVREALLGETLRATGLFNEAYLRHLVDAHQSGARDYSSAIWTTLMFEAF
                     LRNCQTTAQPAPLVRSA"
     gene            complement(15244..16410)
                     /locus_tag="PKX01_02570"
     CDS             complement(15244..16410)
                     /locus_tag="PKX01_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HMZ75035.1"
                     /translation="MSALHPDGRLLVMHVVYSFAVGGLENGVVNLINRMDRTRFRHIV
                     VALTQCEPLFCARVQVPDVEFVALHKPPGQGVKIYPALFRLFRAYRPDVVHTRNLAAL
                     EATVPAWAAGVPVRIHGEHGWDVSDPHGTRAKFRWMRRLYRPFVTHYIALSGQILSYL
                     VRAVGIPDRRAERICNGVDVARFTPRGARRAALEGSPFNDPAYFVIGTVGRLQAVKDQ
                     LNLVRAFARMRQLDATHSESVRLVLAGDGPLRGRIEDEIRTLGLNGRVWMAGERSDVP
                     RMMSGFDLFVLPSQTEGISNTILEAMAAGLPVVATDVGGNGELVADGESGALVPPQDS
                     QRLADKLLEYVRDPLRGRAEGHAGRLRVEANFSLDGMVKRYADLYLALCAAKGR"
     gene            complement(16407..17912)
                     /gene="xrtA"
                     /locus_tag="PKX01_02575"
     CDS             complement(16407..17912)
                     /gene="xrtA"
                     /locus_tag="PKX01_02575"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HMZ75036.1"
                     /translation="MNSRRAVPVAIAGVFLWIIGAYFTTAADIAGVWWRSESYAHGLM
                     VIPIFAWLVWRKREVLATRPLRAWPIWLLPVAVAGFGWLLGQLGSVASVSHFCLVTML
                     VLTLFGVLGREAAGALKFPLLFLLFGIPAGEFLLPVMMKYTALFTVSAVRLMGVPVYQ
                     EGLHFVLPNGRWSVIEACSGFRYLIASVMVGTLYAYLSFRSLRRRLIFTAVAIVMPVV
                     ANWLRAFMIVMLGYFSNNQLATGVDHLIYGWVFFGLVILLMFWVGSFWREDLDELPAA
                     AAVPAPAASIDTAALVRGVLPFALLTAVWPVVAVLLAAPTDRDVTIPIALPAAGGAWR
                     AGGVETGHAFQPRFSGHRGLMEEGYSDGRRTVNAYVAVYANQSQGRELVRAGNVLLSV
                     EDHHWAMLDEGAGPETPFGAWKRSVLSGEGTRIVVWSAYWIDGRLVTSDYVAKGLLTL
                     ARLTGKADTSAYVAMWSRAPDERSAEQALEDAAASLQGPLFQQLQSLDRVK"
     gene            complement(17983..19020)
                     /locus_tag="PKX01_02580"
     CDS             complement(17983..19020)
                     /locus_tag="PKX01_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HMZ75037.1"
                     /translation="MASPISVRRFQAGDSTRWNAFVFDCPDATFFHRIEWRDIIERVF
                     RHRCHYLLAERDGHIVAVLPLVEVNSRLFGHSMTSLPFAVYGGVAGSDADAVAALEAE
                     AETISRQAGVQHLEYRNLRARHEDWPRQDLYVTFRKEILPDEEANMLAIPRKQRAMVR
                     KGIKNGLVSHVDPNADRFFALYSDNVLRHGTPALPKRFFETLLDVFGKDCEVMTVTTA
                     DGKLLSSVLSFYFREEILPYYAGDDMEARDLAANDFKYWELMRRACARGCRLFDYGRS
                     KIGTGPYSFKKNWGFEPMPLSYEYRLFKRDEIPQNNPMNPKYRAFIALWKRLPVGVAN
                     RLGPMIVRNLG"
     gene            complement(19031..19900)
                     /locus_tag="PKX01_02585"
     CDS             complement(19031..19900)
                     /locus_tag="PKX01_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HMZ75038.1"
                     /translation="MNPHVDDARSLVNALTCDVEDYFQVSALAPHFPRSGWDGTPCRV
                     EANVDRILALMDAAGAKGTFFTLGWIAERYPALVRRIAEAGHEIASHGYGHERASDQS
                     PEAFLCDIRLAKAILEDITGLEVRGYRAPSFSVGLSNPWAHDCIQAAGYVYSSSVYPV
                     RHDHYGVPDAPRFTYPVGNSLVEIPITTVRRFGRNWPVGGGGYFRLLPYGVSAWGIRQ
                     VNAGDRKPAIFYFHPWELDPGQPVVLEASAKTRFRHYVNLERTEARFTRLLNDFAWGR
                     MDEVFLSGDTPSA"
     gene            complement(19897..20973)
                     /locus_tag="PKX01_02590"
     CDS             complement(19897..20973)
                     /locus_tag="PKX01_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HMZ75039.1"
                     /translation="MYESHFGLRGKPFQLNPDPSFFYGSRGHRRAMAYLEYGLHQNEG
                     FIVITGEVGAGKTLLVRSLLEKLDPSKIVAANLVSTQIDADDILRLVAAAFGIPSRQM
                     SKSDLLVAIETYLVSVAAAGKRALLVVDEAQNLTPRAVEELRMLSNFQLEDHALLQSF
                     LVGQPEFRAIMQSPQMLQLRQRVIASFHLGPLDATETQAYIEHRLNCVGWRGVPSFDA
                     DALAAIHASSQGIPRRINAVCDRVLLAAFLTEQRRISAAIVEQVVAEMREEQVAPPAM
                     QADTPAAALSAGLAGVDLARLAAEPEALRTLAKAGEGFDTARFEERLALLERTLSTTL
                     NALGQLLQAVQKPSQTAGKETESR"
     gene            complement(20980..22509)
                     /locus_tag="PKX01_02595"
     CDS             complement(20980..22509)
                     /locus_tag="PKX01_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HMZ75040.1"
                     /translation="MSVLTIPLALTSMSVLAQDHQVTFSPSLRLTETITDNVDLSRGS
                     GTGGNASSKSDYITGIIPSMELNSRGGRAQGNLRASVASLFYANNSNLNTSFLTFNGT
                     GSVEAVEKFAYVDVRGSESREVASVLGVRPGDQVTGRANQTNVRNVGISPYLRGHFHN
                     SGVAELRYDINSSSASSGSFQRMQQQMWSGSLVDETAFGQSGWSLAFSDNQVSQTGRR
                     DLHLQSARLTLMSRVTPQLQLRVIGGGEGNNYNSSNTQQSTIVGAGFDWKPSAFGRVS
                     ATWEDRFFGPGYQMSGDYSTGPFVFNAKFSKDVTSTSQALSATSLTTAYDLLMGLLAS
                     RYPDPVARDQAVRTYLTNQGVSTDQQIGISQSVLNNGVFLDRRLQFGASYVGLRRTTF
                     MFSGYRSERSSLVSQTAALGGEFINATRVTDSSGSLMLNHRLTPLTSATADMTLIRTQ
                     ADGSATTPSFTNKTRRATAGLSTSFTRRSSGSLTYRHSRATGTTEYTENALLGSVIIL
                     F"
     gene            complement(22560..23612)
                     /locus_tag="PKX01_02600"
     CDS             complement(22560..23612)
                     /locus_tag="PKX01_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HMZ75041.1"
                     /translation="MSIIEKAVERLEQLQKAAAAASAGAPVEAAPVAGSAEGRIEPVG
                     TPVEQPAPVIAAVTAAVTVEAAPVAEVPLPVPSAAPEASVSSRPRASTARSVEIDLER
                     LASIGVVTPNAPRSLIGDEYRIIKRPLLRNIQGKGAAAVDSANLIMVTSSLPGEGKSF
                     SSINLAISMAMELDHTVLLIDADVSRPSILNILGLPPKKGLMDVLTTDDVELGDVLLK
                     TNIDKLTLLPAGTPHPRATELLASDAMNNLLAELAVRYSDRIVVFDSPPLLVTTESRV
                     LATHMGQIVMVVESNKTTQAAVKQAISTIEACPVKLMLLNKALQAGSGAYGYGYGYGY
                     GYGYGYGYGQSQKENA"
     gene            complement(23622..25178)
                     /locus_tag="PKX01_02605"
     CDS             complement(23622..25178)
                     /locus_tag="PKX01_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HMZ75042.1"
                     /translation="MEDILRQLSVILRGMWLYRWWGLAAAWIVGPIAAATVYLLPDRY
                     ESSARVYVDTQSILRPLMSGLAVQPNVEQQINILSRTLISRPNVEKLIRMADLDLAVK
                     TKEEREALIVGVTKALEIREAGRDNIYTLAYKDTNAERAKRVVQSLVSIFVESGLGDK
                     RKDSDTARKFIEEQIKTYEQKLEEAENRLKEFKLRNLSTMGAGGRDYFSKMGEVNEQL
                     NQARLALREAENSRDALKRQLAGEDPVLLPENPSSSALASVSVPELDGRIEAQKKNLD
                     SLLQKYTDQHPDVIGTRKVIEQLEAQKNQEIQSRRKAGPSTVGSINSNPVFQQMKMSL
                     AEAEANVASLKARVSEYEARQQNLSSASRMVPQLEAEYTQLNRDHDINKKNYDALVAR
                     RESATMAVEMGATSGVADFRLIDPPTLPSKPAAPNRLLLMPGAGLAALLLGAVVSFMM
                     SQIRPVFTDMQVLRDVTGLPVLGAVSMIADPERMGTQRRGRIVFLGGVGALVGLFGAM
                     TVWLLLAKVA"
     gene            complement(25237..25842)
                     /locus_tag="PKX01_02610"
     CDS             complement(25237..25842)
                     /locus_tag="PKX01_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HMZ75043.1"
                     /translation="MKRIVTTGLLTLAVVLATGCATKLPPPPATATTPEYNYLIGPGD
                     KVNIVVWRNPELSMTVPVRPDGKITTPLVEDLPASGRDSSTLAREIEKALSKYIRDPV
                     VTVIVTDFVGPYAEQVRVVGEVARPQTLSYKQKMTLLDVMIAVGGLTDFADGNRAKIL
                     RTSDNNAEYGVRLRDLIKRGDVSANAEVKPGDVVIIPQSWF"
     gene            complement(26035..27342)
                     /locus_tag="PKX01_02615"
     CDS             complement(26035..27342)
                     /locus_tag="PKX01_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75044.1"
                     /translation="MKTTTLARNAAALLAALALCTGTPVHADEEGDDAGLDVADEGAR
                     TTGVLQFDLARTYASPEHWSMARSRLELGATGGSAGLKWKISGRADADAVFAIDNGFY
                     PDAVRRDQQLGFDLRETYVDFSVGDVDVRLGRQHIVWGEMVGLFFADVVSARDLRTFY
                     LPDFDQLRIPQWAARGEYYFGDTHAELIWIPVPSYDRIGKPGAEFYPIPAGVPVLGEV
                     KPSNSLGNTNWGGRLSTLAAGWDVSGFYYRSMDVSQTFFRLGSITSPVFEPRHLRISQ
                     AGGTVSKDLGAFVLKGELVQTRGREFNTLDPTVDFGLIAQNTLDYVVGVDIPAFRDGR
                     VNLQYFARRYLDFDPRIGQDRIEGGASVLLNTKLSNNWEAEALFVSSVNRTDFMFRPK
                     LVWSASRNLKVVFGVDSFGGQSTGLFGMYDTRDRAYTQARWSF"
     gene            complement(27348..28094)
                     /locus_tag="PKX01_02620"
     CDS             complement(27348..28094)
                     /locus_tag="PKX01_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75045.1"
                     /translation="MSIIRVENVSKTYLLGEQTVTALEGVSLVVEPGVFMAIAGPSGS
                     GKSTLLNIMGCIDTPSKGRVEIEGIDVSGQTPDSLADVRARTLGFVFQTFNLLPVLSA
                     QENVEYPLLQMTELTKEERAERVAFYLNMVGLTKYADHRPNQLSGGQRQRVAIARALV
                     THSRVVLADEPTANLDRRTGESILNLMREINQNAGTTFVFSTHDKRVMNRADRLVRME
                     DGQITALGMKREGSWIFVQDRRKPEDDPEI"
     gene            28137..28922
                     /locus_tag="PKX01_02625"
     CDS             28137..28922
                     /locus_tag="PKX01_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HMZ75046.1"
                     /translation="MTPQNPLWPRRNLLFRLLPAALLALSSLPIHAEDDEFAKNLVAK
                     ADEVRFPREGFQVDIIVDSTSPGEDPDQHKYRILSKGNENTIVQTLEPASDRGQAMLM
                     RGRDLWVYLPNVSQPVRLSLAQRLTGQVANGDLARANFAGDYDPKLLRTDTLDGDKVH
                     VLELRANDRSVTYSKVLYWVRASNNYPVRAEFYSASDRLMKTCRFEKYQTLGGRARPT
                     QLIMEDALRKGDMSVLHYSHMALKDLPDRIFTKQYMDKLNRVD"
     gene            complement(28937..29617)
                     /locus_tag="PKX01_02630"
     CDS             complement(28937..29617)
                     /locus_tag="PKX01_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMZ75047.1"
                     /translation="MTKVDPREARALLRSCNQGALGTRSVTMPGYPFVTLLPYALDGG
                     GAPLFLISALAEHTRNLLADSRASLLVAETSTSPLEQPRATLMGHVVPCDLEPAARER
                     YLSYRPEAADYLQLGDFRFFRMGIERVRLIGGFARMGWCDAPATGWQPTATVERELTA
                     ELARTAPAGVTILGVDADGADLRIAGVVRRLDLDEPAGGAEALRQALYAGLPRIAPPA
                     GEAPVTLA"
     gene            complement(29625..30371)
                     /gene="rlmB"
                     /locus_tag="PKX01_02635"
     CDS             complement(29625..30371)
                     /gene="rlmB"
                     /locus_tag="PKX01_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMZ75048.1"
                     /translation="MSESASSRLIYGFHAVLAKLRHDPEAVFEIYIDAQRSDGRARDF
                     LRQAEQLGVRLIPSEGERLDKMVGTRRHQGVVARIDGRRREVMLPDLLDSLDGAALLL
                     VLDGIQDPHNLGACLRVADAAGAHAVIAPKDRAVGLNATAVKVASGAADTVPYITVTN
                     LARALREMQEAGVWVLGTAGEADKSLYEVEQKASVAWVLGAEGDGLRRLTRETCDELV
                     RIPMYGSVESLNVSVASGICLFETRRQRGA"
     gene            complement(30364..32940)
                     /gene="rnr"
                     /locus_tag="PKX01_02640"
     CDS             complement(30364..32940)
                     /gene="rnr"
                     /locus_tag="PKX01_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ75049.1"
                     /translation="MPKPKTPKKLSAFRRSDPFYEREVATYEFPLPSREFIEQVLADR
                     GVPLSLADLVDAMDIASHEREFFERRLFAMERDGQLVRNRRNAYLLPNKADLIRGTVQ
                     GHPDGYGFLVPDGEGQDIFLGPNEMREVLHGDRALVRIAGTDRRGRPEGKLVEVLERA
                     NTRIVGRVLVENGVTLVVPENRRIAQEILLASAGKAKVTAGQVVVVELVQQPTRYAQP
                     VGRVVEVLGNYADPGMEIEIALRKHELPFEFPSAAKEQTRRLPDKVRKTDWKGREDIT
                     GLPLVTIDGETARDFDDAVYCERQGRGFRLVVAIADVSHYVRPGSALDDSAYERGNSV
                     YFPRRVIPMLPEKLSNGLCSLNPQVERLCMVCDMAITQTGTIKQFRFYPAVMFSHARL
                     TYTQVAAALYDQDPAALETVGALLPHLQNLDKLFRVLLKARAKRGAIDFETQETRMIF
                     DDQGKIEKIVPESRNDAHRLIEECMLAANVCASEFLQRHEHPALYRVHEGPTPEKLVK
                     LREFLGSFGLQLPGGDDPHAGDYARLLASLQGRPDLQLLQTVMLRSLRQAVYSPDNLG
                     HFGLAYEAYTHFTSPIRRYPDLLVHRAIKAVLTGERYQPPQESGEWEAIGLHCSMTER
                     RADEADRDVENWLKCYYMRDRIGEEFIGSVSAVVPFGLFVALDDVFVEGLVHISDLGN
                     DYYHFDDAHHTLVGERTGKRYRLSDRVRVQLVRVNMEAGKIDFRLLEGPLPARQPEAE
                     RGDAAAETPAPARRRRVANGTAGAEVMAPIVSPESPVQTAPRRARSASKTGDAGGSPV
                     KPAKSVQTSKAAKPSKAAKASKPSKPTKTSSKPAGSRASTSTGPSKRAAGGTKKKAKS
                     RV"
     gene            32980..33064
                     /locus_tag="PKX01_02645"
     tRNA            32980..33064
                     /locus_tag="PKX01_02645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33014..33016,aa:Leu,seq:cag)
     gene            complement(33239..34549)
                     /locus_tag="PKX01_02650"
     CDS             complement(33239..34549)
                     /locus_tag="PKX01_02650"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMZ75050.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDQAEGVVRFQGGHNAGHTL
                     VVGSNEYKLNLVPSGIVREGVKCFIGNGVVLDIHHLLSEVRGLESGGIKVRERLRISP
                     GCPIILGYHAAVDRAREARKSAGDKIGTTGKGIGPTYEDKVARRALRVYDLFNPARFA
                     EKLKELLEYHNFVLTQYLGADPVDFQSVYDQAMADAEEIKPMVCDVSADLYALNKAGG
                     NLLFEGAQGTLLDIDHGTYPFVTSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVG
                     GGPFPTELDFETPGTPGYQMSTVGREFGTVTGRKRRCGWLDLAALKRSIIINGVSGLC
                     ITKLDVLDGLTELKLCTGYMLDGKRVDLLPMGADEVVRCEPILETMPGWTGSTFGART
                     WDALPPEARAYLHRIEEICEVSIDVISTGPERDETILKRHPFGA"
     gene            complement(34554..35714)
                     /locus_tag="PKX01_02655"
     CDS             complement(34554..35714)
                     /locus_tag="PKX01_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HMZ75051.1"
                     /translation="MRWVLPEHIQDVLPAEAEKLESLRCRLLDAFRLNGYQLVMPPLL
                     EYLESLLTGAGQDLQLKTLKLVDQLSGRTMGVRADITPQVARIDAHLLNRQGVARLCY
                     CGSVAHALPSSLTATREPLQLGAELYGHAGLEADAEVIRLLAESLQIAGVTTSRIDLG
                     HVGLFRALAARAGLRDEQQESLFDCLQSKDIPGLRDLLGDAPEAVRSALLVLPTLYGG
                     PEVLERARGVLSGLDDADVRGALDDLESLSRLLADLPISFDLADLRGYHYHSGVMFAA
                     YGGTSPVALAQGGRYDRVGTAFGRGRPATGFSLDLRELAAQTPDGAGRGMILAGECSV
                     PGFAQEVARLRASGEIVVIELPGHDGTWREAGCDRRLVLRGGAWQVEPLQGE"
     gene            complement(35730..35915)
                     /locus_tag="PKX01_02660"
     CDS             complement(35730..35915)
                     /locus_tag="PKX01_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HMZ75052.1"
                     /translation="MGSSLFLAFALMLVLEGLLPFVAPKMWRETFTRLVRMSDGQIRF
                     IGLSSMLVGLIVLIVFS"
     gene            complement(35915..36838)
                     /gene="hflC"
                     /locus_tag="PKX01_02665"
     CDS             complement(35915..36838)
                     /gene="hflC"
                     /locus_tag="PKX01_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HMZ75053.1"
                     /translation="MRDRAPFFIGVLLFVVALMSMSLFSVDQRQYALVFQLGEVKGQL
                     TEPGLHVKIPLIQNVRYFDRRILTMDSNEPERFITSEKKNVLVDLFVKWRIVDPKLYY
                     ISVGGDESRAKTRLEQTVNAGLREEFGKRTVHEVVSGEREKIMDEMRVKADQDARKIG
                     VQIVDVRLKRVDLPLEVSESVYRRMEAERKRVANELRSQGAADAEKIRADADKQREVI
                     VAEAYREAQKVKGEGDAKASAIYAQAFSQNPEFFAFYRSLDAYRNSFKSKSDVLVLEP
                     NADFFKYMKSAGGRGTATAPADANKGGKSAK"
     gene            complement(36838..38103)
                     /gene="hflK"
                     /locus_tag="PKX01_02670"
     CDS             complement(36838..38103)
                     /gene="hflK"
                     /locus_tag="PKX01_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HMZ75054.1"
                     /translation="MSINDPKWGNRGGGDNQGPPDLEELWRDFNRRLSGMFGRQPGRG
                     GDGGGDSGGGDRMPELNFRQFGGGVGLILALAVVIWLASGFYIVDASQRGIVLQFGRF
                     VEATEPGLRWRLPYPVQTHELVNQTGVRTVEIGYRGSERNKVLKEALMLTDDENIVNI
                     QFAVQYILKDPQQYLFNNRNADDSVIQAAETAVREVVGKSKMDFVLYEGREQVAANAH
                     KLMQDILDRYATGIQISKVTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDV
                     IPRARGTASRLLEEADAYKGRVVAQAEGDASRFRQIYTEYAKAPEVTRQRMYLETMQQ
                     IMSNSSKVVIDSKGSGNLLYLPLDKLIAATAAQASGAVTPEAAAQTRPQVPAAAVAAD
                     NSPAPPRPDALSGESRTREAMRNRERGDR"
     gene            complement(38155..39291)
                     /gene="hflX"
                     /locus_tag="PKX01_02675"
     CDS             complement(38155..39291)
                     /gene="hflX"
                     /locus_tag="PKX01_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMZ75055.1"
                     /translation="MFDRPESGNRAIIVQVDFGQGGIDERLAEVSLLASSAGASVVGV
                     VQGRRQAPDPAHFVGTGKADEIGLLARMNEADLVIFNHDISAAQQRNLEKRLNCRVVD
                     RSALILDIFALRAKSHEGKLQVELAQLEHMATRLVRGWTHLERQKGGIGLRGPGEKQL
                     ETDRRLLGERVKLLKDRLRKLERQRAVRRRARERRDVLSVSLVGYTNAGKSTLFNALT
                     KAGAYAADQLFATLDTTSRRLYLGDEAGTIVLSDTVGFIRDLPHALVAAFHATLEETV
                     AADLLLHVVDSASDDRDGQIDAVNGVLHEIGAGDIPQILVWNKIDATLASPEVQVDAY
                     GKISRVLLSARTGEGLDLLRQILADVALAHKAALAAPSDQPQLG"
     gene            complement(39361..39603)
                     /gene="hfq"
                     /locus_tag="PKX01_02680"
     CDS             complement(39361..39603)
                     /gene="hfq"
                     /locus_tag="PKX01_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HMZ75056.1"
                     /translation="MSNKGQLLQDPFLNALRREHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNIQQEQNEAAS"
     gene            complement(39707..41035)
                     /gene="der"
                     /locus_tag="PKX01_02685"
     CDS             complement(39707..41035)
                     /gene="der"
                     /locus_tag="PKX01_02685"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMZ75057.1"
                     /translation="MKPTLVLVGRPNVGKSTLFNRLTKTRDALVADFPGLTRDRRYGL
                     GRVDEREFLVVDTAGFDPVAKDGIVHEMAKQAEQAIAEADALLFMVDARSGLTPHDEQ
                     IAIRLRRAGRPTFLVVNKAEGMNRAMVSLDFHALGLGEPYCISAAHGDSVKALMELVV
                     DTFPSDGGEDEAPTQGPRVAIVGRPNVGKSTLVNALLGEERVIAFDMPGTTRDAIEVP
                     FERNGKHYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAVEQCNVAVLVLDASQEISDQD
                     AHIGGFVVEAGRALVVAVNKWDDVDDYRRERIKQDIARKLPFLGFARFLYISALKGQG
                     IPPLLRSVDGAYAAAMTKLATPKLTRLLLDAIVKQQPPRHGGFRPKMRYAHQGGMNPP
                     IIVIHGNALDHVPASYVRYLERSFQEAFKLQGTPLRIQFKSSHNPYADRD"
     gene            complement(41049..42185)
                     /gene="bamB"
                     /locus_tag="PKX01_02690"
     CDS             complement(41049..42185)
                     /gene="bamB"
                     /locus_tag="PKX01_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HMZ75058.1"
                     /translation="MRRLGRVAALIAAFGLLGCSSLNPFSSSGPKIPPLKPITDSVKL
                     GEDWKLRVGSAGTSVFSPLVVGSAVYAAAADGTVVRVEDGRTVWKVSLKTGLSGGVGG
                     SAEMVLVASPKGEVYALDPASGAIRWQANVNAEVLAAPALGDGVVVVRAADSRLFGLE
                     PADGKRRWVYQRATPALALRSAAGVVMADRAIFAGFPGGKLAAVNVNTGALMWEGTVS
                     VPRGATELERVADISSPPVLNGRAACGVAYQGRVACFDLANGSTLWTRDLSSARGLDL
                     DESHVFVTDDKGAVHALDLTSGASMWKQDQLAGRSPGRPLAFRDMVAVADVDGIVHVL
                     RKDDGTFVGRQRTDGSPVLADLRRVGSDLLVQTRDGSVFAFGVQ"
     gene            complement(42182..42835)
                     /locus_tag="PKX01_02695"
     CDS             complement(42182..42835)
                     /locus_tag="PKX01_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ75059.1"
                     /translation="MAAYDLEEQEQISQIKAWWDTYGNLVTGIALAAAVAAVGWQGWN
                     WYQGKQSGEASVLYAAVQKAAAENKPQQAREAAGMLIEKYSGTAYADLGALLSSRVQA
                     DAGDLKSARAQLGWAAEKANDPALRELARLRLAAVLLDEKSYDEALAQLQAKPSEALE
                     ARYGDLRGDILAAQGKVADARGAYKAALEALASATKPEAVAFKEVVQAKLDGLGGAQ"
     gene            complement(42822..44117)
                     /gene="hisS"
                     /locus_tag="PKX01_02700"
     CDS             complement(42822..44117)
                     /gene="hisS"
                     /locus_tag="PKX01_02700"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMZ75060.1"
                     /translation="MSKTLQAVRGMNDILPDDAEVWEQFEDIVRDWLRSYGYRPIRMP
                     IVEPTPLFKRAIGEVTDIVEKEMYSFVDALNGEQLTLRPEGTASCVRAAIQHSLIFGQ
                     PRRLYYHGPMFRHERPQKGRYRQFHQVGVEALGFDGPDIDAEHILMCARLWDDLGLED
                     VKLELNSLGSADERAAHRAALIAYLEGYKERLDEDGQRRLHTNPLRILDTKNPDLQDI
                     VEAAPRLMDFLGDESLRHFDGVQALLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTRL
                     GAQGTICAGGRYDGLIAQLGGKPAPACGFAMGIERLLVLWQESGFIPEPLTPDVYLVH
                     AGEAAQRAAFRAAEALRGTGFTVTLHCGGGSFKSQMKRADASGATYAVVIGDDEAAAG
                     EVSLKPLRSGGEQIRIPMEILPETLADRLLELEDDDGGL"
     gene            complement(44142..>44282)
                     /gene="ispG"
                     /locus_tag="PKX01_02705"
                     /gene_synonym="gcpE"
     CDS             complement(44142..>44282)
                     /gene="ispG"
                     /locus_tag="PKX01_02705"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607106.1"
                     /note="catalyzes the conversion of 2C-methyl-D-erythritol
                     2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl
                     diphosphate; involved in isoprenoid synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HMZ75061.1"
                     /translation="GESPAAPVFEDGVKTLTLRGDNIAGEFKAIVERYVARTYPKKGA
                     AA"
BASE COUNT         7951 a        14440 c        14408 g         7483 t
ORIGIN      
        1 gcacccgtgt ttgccggcgg ggccgcggtg cccggcgggt cttcgggcgc gggctcgggc
       61 tggggtcgcc acgcgccgat gcgctgcagc ttgttgcgca gtccgggcag cggatgtccc
      121 agggcgtagg ccttgtgggc tgcttcgagc gcctgctggg gcttgtccac catcaacagg
      181 cccaggccca ggttgtactg atagttggcc gagtccacct gaagctcgcg ggccttggcc
      241 atctgttcgg cggcttcggc cttcatgccg cgctggacca gccaaagacc gttgatgagg
      301 tacatcaggc cgtcgtcggg gcggaactga atcgcccggg aaacccagca atcgacagtc
      361 tccgtggtac cggtgggcgt cggggtcttc tgcttgagcc ccagcttcac catggcgttg
      421 agcgcccgga agtggttggg gaaatagcgc agggtgaatt ccagatcgtg gcccacgtag
      481 gcgctgctct tgcccttggt gagggattcc acgccggtat cgaagtggta gttctcaact
      541 cgcaggagct tgtccggcgg cgccacacgg taatcgtagg ggccgaactc cagtgccggg
      601 accggctggc aggtcccgtg ctcaccagcc attgccgcgc tggccaccaa catgccggcc
      661 accacccatc ccgcaacccg cccgcggcgg ctaaggacgt gcatcgtttc tctggtcacc
      721 atggaagcct cgtcaatgtg tcgagcctct gccgcggatc aggcgcgggc agacgccagc
      781 tggggcgcgt cgacgttcag ataattgcgc aacaggcgca gggagaattt gcgcagattg
      841 tgatcccagg gcgtgaagcg cggcaactcg tacagcccgg attccgaacc gaccgagccg
      901 tgggacgtgg tgaaggctgc ttcgtagccg agttcccggg cgagacgggc gtgcactgcc
      961 gtgtaatcct tgcccggccg accgttggga taagcgaaga agcgcggcgg acgtcccagg
     1021 agttccgcca gacgggcccg gtcgcgggcc atctggctgc gggcctcgtc ctcgtccagc
     1081 accgccagga tggggtgcga aaccgtatgt ccgccaatct ccatgcccgc gtcccgcagg
     1141 ccgcgcaact gggcgctgct catcatcagg tcctcgggca ggcggctccc ggtctgggcc
     1201 acgagccgtg ccagcgccgc cttccggcca tcgaggtcga ggtatttgag cttcaacagc
     1261 aaggcctcgg ccagcacctg acgctgcaca acggactgcg tcgcgcacat ccccatgtcg
     1321 agccaggcca gatccagggt ggcaccgccg tactggcgca gggtctcgat gaccgcgtcg
     1381 ttccacatca ggccgccgtc gaggaagccg ctggccacga agaaggtggc ttccatgcca
     1441 tgtcggcgca ggatcggaca ggccactgtg aagttgtctg cgtaaccgtc gtcgaaggtg
     1501 atcgccaccg accgtgccgg cagggtgccc gcagccctgc gggacagagc ctcggccatg
     1561 ggcaggacgt ggaattcccg cccgagcatt gtcagcaggc gatcaaagcg gtcggcgtcg
     1621 atctcgtccg gattcatcgg gtccggttcc ggaacgacgc gatgaaagat cagcacggac
     1681 aggcgccggc ccttgccggg caacagcgac gcgaggcctt cggccttcag cgccagggaa
     1741 cggggaagca gacccatgaa ttcctctcgg gagacggctc aggcgccccg caggcgccgc
     1801 cgcgccccac gcaggagttc cttgaaaccg gagatttccg cctcgaactc gatgtcggaa
     1861 atatcgtccg cgtcgagaaa gcggtgcagc gcaagacggg gcgtcgtccg ttcgttgagg
     1921 ccggcgatgc aggtggtggc cgacacgacc cccgccgcct ccagcaccgg aaacgacgcg
     1981 gcgcgccatt cgccgctggg atagcacaga tgccgcgccg cagccacgcc gcaggcagcc
     2041 agcgccgccc ggttatcgtc gatctcgcgg cgcagttggg ccggatcggc cgggtagcgg
     2101 tgccggtggg tgtgcagctc gatggacagc ccggccgcct gcagcgcacg aacctgttcg
     2161 gcggtgacaa tccggaagcg ctcgtcgagc ccggcggatt ccggttcgag cacccctgcc
     2221 gcgcgggcgt tccgcaggaa ggcccggcgg gccggcttgt cgagacgctg gtagcgggcg
     2281 atcggcccac cctcccccgg cggcagcagc gccgcaagcg cgtcggcccg ttccgggtga
     2341 cgacgcaggg cacgccagat caggtactgg gccaccagat ccgccacggg acgggcagcc
     2401 gtctggtagt gggtggtgag gtagagcatc gccggaatcc ggcgcgcggc cagggcaggt
     2461 gccgcatgac gcagcacgct gatgaagccg tcgtcgatgg tgatgaccac gtcccgcaag
     2521 cgtgccgatc cggcgtccag tcgacgcagg gcctcatcca gggtgatgac ccggtagcct
     2581 cgccgggcca gcaggtcgag gcgccgcgca aatgttgccg gctgcatgaa cagctgcggc
     2641 cggaaaagat gctcgtcctc gaaggcaaac gcgtggtagc agaggatgct ctggcgcccg
     2701 cggtagagcc agctggccag ggtgaacaaa cccagcaggc gggcgagacg atagaaggcc
     2761 ccgcgcacct tacagccccc cggccggtcg cggcctgggt acggcagggg caccgggcgc
     2821 tgtgacggcg tccggcacct tgcggcaatc ccgccagagc agacccacga tggcgacaag
     2881 gtaatagggc acgtcgaagt tcaccagccc gaggaaggtc ccccccacgg caaagctgag
     2941 gagcgagatc tggatgcact gggccagcag cttgagccac tgttgttccg gatcgttgcc
     3001 cacctccttg atcatccggc cgcaaaagcg ccaggaggtg acgcccatgc cgatgtagaa
     3061 gaagaagccg aggatgccgt gctcgccgag ggcctggaag tagatgctgt ggggcgaata
     3121 gggcagcagc cactcggtga cggcgtagcg cccccagacc tcccaggtcc agaagtcgaa
     3181 ccctgcgccc catggattgt gggccaccat gttcaggatc atctgccagg tgtagaggcg
     3241 gctctgggcg gagtggtcct cgtgggacga gatcgtgttc atccggttcg accaggtgtc
     3301 cggcatgaag gccgccaaac cgatcccgat gatggcgaac agggacaccg ccagcaccgg
     3361 tcgcttgctg cgcgcaccga ggatcagcgc catcgcgctg atggccagca gcgcgccgcg
     3421 ggactgggag ccgagcaccg agaaggccac caggatcagc gagatgctga gcaccgtccg
     3481 ccaccaggcc cgctcggcgg tctgacgcat gaagtacatg aggggcatga tggtcacgag
     3541 ggccagggcc agctcgttgt tgccctcgaa aaagctccca gtcggccccc acacccgctc
     3601 gccaccgccg gaacggatcg taaacagccc ccccttgatc ccgtagaagc cgatggacag
     3661 ggtaatcacc ttgatcagat cgaggatctg cttcttgccc ccgatgagcg cgatggtcac
     3721 ccacagcatc acgtgcacct tggtcacctt gacccacatg tcgaaggcgt cgcccacgtt
     3781 gttcagggcg atgatggtcg tgaagcacat caccgcgtag aagtaggcca ggtagcggat
     3841 cggcgtgatc cacgggatgc ggtgccgctc cttggcggca aacagcgcga tgagcgtggc
     3901 gccgcccacc agctgcgcga acggcaggga ataggcgaac ccgaaggtca ggcggtgcgg
     3961 caccatcatg ctcacccatg cccaggcgag cggccccagg gacgggcgga agaagatgct
     4021 cggcaccagg acgagcatga tcagggtcag ggctacgtca cgcacgcggg gctcccgccg
     4081 gcgtccaggc tgcgaccggc atcatgctgg aggggaaaaa catcgtgtcg cccatctcag
     4141 cgtttgacgt ggctcacgac atagcgggtc ttgcccgatt tctccggcgg gatctcgctg
     4201 accggcgtga tgtccacctg cacgcccgcg cccaggcggg cggcgtagtc gtggcggatg
     4261 cgctggcgct cggcctcgcc gaatcggtcg tccgccacca ccagcacctc ggtacggccg
     4321 atgtcgtgct ggacgatctt gaactgctgc acgccgggca gatcccggac ggtatagatg
     4381 agcgcgaggg cgtgcatcac cgtgccgtcc gcgcccacca cgaagtcggt ggtgcgcccc
     4441 tgcacctcgg ccaggatggg caggttcaca ccgcatccgc aggtttccgt gctgagcacg
     4501 ccgatgtcgc cggtgcggta gcgcacgaag gggaaatccc gcgtggcggt gtgggtcacg
     4561 acgatctcgc cgggcgcgcc cggcgccacc ggccggcctt ccgggtcgat ggtctcgacg
     4621 acgatgtcct cggccgacag gtgcaggtgg ccgtgggggc actggtgggc gatgaagccc
     4681 gcgtcccgtg ccccgtagcc attggccacc gggcagccga agatttcggt gatggactgc
     4741 ttctggtgtg cgtagagcat ctcggaggtg acgaaggcga cgcggatgcc caggtcgtcc
     4801 atcgggatct tccacaggcg tgcccgctcg gccagatggg cgagggccga aggatagccg
     4861 aagagcatgc gcgggcgctt ggcgcggatc atcgccagca tgtcccgcac cttggcgtcg
     4921 gacatctcga aggccggcag cagccaggtg cgcagcaggc tgtcgcggac ggccttgacc
     4981 cggtcctggg cacccagttc gatgggcgag ccccacagca cgatctccgg atcgccgata
     5041 tccacgtccc accaccgcgt cgcccgccac ttggcggcca catcatggct cttgcgcgcc
     5101 ttgccgatgt agaagatcag cggttcaccg ctggaaccgc cggtgttgta gcggctcagg
     5161 ggaccggcgt cgtcggcctt cagttcgtcc acgtgagcgc ggatgacggc cttggtcatg
     5221 aagggcagct tgcgcagatc cgccgcaccg gtcacgccgg ccggatcgaa accgtgttcc
     5281 cggaacagcg tgtggtagta cggcacatga cggccgatgt cttccaggaa ggccttcagg
     5341 cggcgggcct ggatcgcctc gatctcggcc cgcggcagac gctgggtgat ttccagatct
     5401 ttgcggcggg caacggagtc gtgcttcttg agtcgttcgt gcaggggaaa gagcaccccg
     5461 gagcacacgc gcgtatagag tcccatggtc ctgtcctgct agcggttgcg cgcgagccgg
     5521 cgatagacgg ccagccactt ctggcgcacc accggccacg catactgttt gacttcgtcc
     5581 cgcgccgcgg cagcgagccg ttcggccaga ggccgatccc gcaggatgcg cagcatcgcc
     5641 tcggccatgc gcgccggatc gcgggccggc accagcagcg cggtacggcc gtcctcggca
     5701 atgaagggca cgccgcccac gtcggtggac accaccggca ccccgctcgc gaaggcttca
     5761 aggatcgaga tgggcatgtt gtccaccgtg ctcgggttga gcatcacgtc ggctgcggcg
     5821 tagagggccg ggatctgggc attgtcgatg cgcccggcaa aggtgaccgc ctcggcgatg
     5881 cccaggtcgg cggccatggc ctgcaggcgg gtcagctccg ggccgctgcc ggccaccgtg
     5941 aggcgtgccg ccggcagcgc tgcacgcact gcggcgaaag ccgccagcac gctggggatg
     6001 tcgtagatcg gctccaggtt gcgcgtcacc accacgtggg gcgcgtcgtc tgccgtgcgc
     6061 gtgcggggca cgccgaagcg ttccaggttg atgatgttgg gcacgatctc cgacgcaaag
     6121 ccgaagcgcc ggaacacccc gtcaagaaag gccgacggga ccaccagcgc atccgcggcc
     6181 gcgagggtgc gatgcacatg gcgcggcgcg gcggcaaaga agggctccgc ctcgcccccc
     6241 cggtaattga cgatgaccgg agccccccgc aggcgggcaa tccagagcgc cggcgcggca
     6301 aacaggtgcc aggcccagcc ggaattggcc agcacatgca tcacctggca gcgcccgctc
     6361 gcccgccaca gggcccacag ataaggcagc agccggaaga aggcgcgcaa caccgggatg
     6421 ctgccagccc actcgggctg atacggcgcg ttggtacgga ccagctccac ctccgcgcct
     6481 tcggcgcgca gcaggccgag cagctgctgg cactggttgg ccatgccgcc ggagggcggc
     6541 ggaaggggtc cgacgacggc cagacgcagg ccggaatagg tgggttgttc catcacaggg
     6601 ggttctcgcg ccggcctgaa cggcgccatg ccagaatctg aacgagcatc aggagggcca
     6661 gcacggcctc gaccgcagcg atcaccctga tctgggtcgc caggcgggtc atccgggcca
     6721 ccagggcctg gttgctgggt gtctcgacga tgcggacgtg gggatgcacg ccggcgacga
     6781 tggcacccag ctgctccagg gagcgggaca cgtgcccgag ggccgcgacc accggtgccc
     6841 gctcgtaatc gaacaccgcg ttgccccacc acaggtacca ctggtccatg ccgtgacggt
     6901 agtaggccag cagcttgtcc tcgtacttca tgccagcgcg ccccggaccg gtcttgacga
     6961 tccttaccgc cgggccctcg ccatggagga atgcccggaa ttcggcgacg ctcaggatct
     7021 cgcccgaatc cgcatagacc ggatgaaaat tgttgaagac gtccaggaac atccagcgct
     7081 ggtgctgccg gtcgaagacc tccaccaccg catggccgtg cccgccgaag ccgtcaaagg
     7141 aaaaccccca ctcccgcgcg aacagaccgg cggcccgggc caacgccaga tacaccgcag
     7201 tgtagtcggc acagtagcca gcgcctttcc ggatggcatg gtaggtggtc tgcaggtcgg
     7261 actggatggg gccctcctgg tcggcgtgct ggctcaggtg ggccgacagg gccagcgcct
     7321 tggcgcggtc gtccggcaag gccgcgatgc ccagattgcg caccacgtcg gcgaacaggg
     7381 gctcgaccgg gccggcatcc tgcaggaaac cggccggcac ctgcgcgggc gtccacgtga
     7441 agtcggcggg cgaggacggc tccacgagca gtgcattacg caggcgcacc gcctcggtgg
     7501 acgggatcgc ctgcaccccg agaaaggccg ccaggatcgc catacacacc acggtggcga
     7561 gcagcagccg gggcatcgcc cggccggcca cccggcgccg gagagctgtc accagcagag
     7621 tccctcgacg tccgcctgga tgtccgccac attgggcaga ttgaccaggt cgatgatctt
     7681 ctggccggga cgggccagac gggccaggtc cgcgaaaact tccttactcg acaggcctac
     7741 caccagcacc tcggcctcgg ccaccacatc ggcgagctcc ggcttgagga gttcgccgat
     7801 gtggggcacg tgacgctcga tgaagctctt gttggcgccc agcaggcggg acagatgcac
     7861 ttccggatcg tagatggaga gctgcacgcc cttgcccagc agctgttcgg cgagggacac
     7921 cagggggctt tcccgcagat cgtcggtgcc ggtcttgaag gacagtccga cgaagccgac
     7981 cttgcgcttg ccggtttcga gcaccttatt caccgccaca tccacgtgat tgcggttgga
     8041 ctgcatgatg cccgccagca tggggatctc cacgtcgtgg cgcttggcca ggtaggtggt
     8101 ggcgcgcagg tccttgggca ggcaggagcc gccgaaggca tagccgggct tcaggtacgc
     8161 gcgggagatg ttgagctggg tgtcctggca gaccaggtcc atgacctcga agggatcgac
     8221 gccgagggcg gcgcacaggc gcgcggtctc gttggcgaag gtgatcttga gggcgtggaa
     8281 gttgttgcag cagtacttca tcatttccgc ggaacgcacc gaggtcttga ggaagttgca
     8341 gggcagatga ccgaagagcg cctttagacg ctccgccggg tagtcgtgat tggcgcccac
     8401 caccgtaaag ggcggcttgt cgtagtcgcg gatcgacgag ccttcccgca ggaattcggg
     8461 ctggaagcac agatggaagc cctctccatc cttcttgcca gagccgttct cgatgatcgg
     8521 acgcagcacg tcttccacgg tgccaggcac cagagtcgaa cggaacacca ccacgtgggg
     8581 cgcggccttt tcgcggatcg ccacgcccat gctctcggcc agacgcagca cggcgccctg
     8641 gtcctggctg ccgttggcgg ccgacggcgt gcccacgcag accagcgaga tgtcgctgtt
     8701 gagcacggca tgcacggcgt cggtggtcac agtgacacga ccggcggcga ccacgtcctt
     8761 catcaggtcc accatgccct cctccaccac cggggtcttg ccctggcgga tcaggtcgag
     8821 cttggtggcg tcgatgtcga cgccgatcac gtcgtgcccg tcacgggcca ggcaggcgag
     8881 cgacaccgct ccgacgtaac ccaggccgaa aatgctgatc ttcatgtgcg cttttccttg
     8941 ctttcctgaa attgagtgat gcagcgtccg atgatccggt caagccgcgc catggacgca
     9001 ggccaggcgt ggtggctgag catccgcgcc cggccggcct cggccagacg gcggcgctcg
     9061 accgggtctg acgtgatgcg agcaatagcc gcagcataag cttccggggt atcggcgacg
     9121 aggaaatgct tctcggcttc cgcatcgacg ccgcccgccg cgatggaact ggtgacgacc
     9181 ggcacgccca tggccatcgc ctcgagaatc ttgttctggg tcccgcgggc aatcgcgagg
     9241 ggcgccacca tcagcgccga gctgcgcacg taggggcgca cgtcgggcac cgagccggtg
     9301 acggtcaccc cgtccagttc gcccagcttg cgcatggccg gggaaggatc cgcaccgacg
     9361 atgagcagct tcatctgggg ccgcgccgct ttgacccgcg gccagatgtc ctggcagaaa
     9421 cgggtcatgc attcctggtt ggggtaatag tccatccgcc cgatgaaact gatggtgtcg
     9481 ggatcgtaga ccgtgtcgtc agggcagaag aagcccgcgt ccacgccgtt cgggaaccag
     9541 tcggtggcga ggccggtgcc gtagctgtca agggtttccc actcggcccg ggtggtggcc
     9601 gtgcccagat cgaacttgcc ggcgaggcgc ttttccgcgg ccagcatctt gaggccttcg
     9661 aggcggtagc cgagggacag ggggaagggc ttgtagtggg cgtactcaag ccacttctgg
     9721 gaatccatgt cgccgtagtc gatgatcttg ggcacgtcgg tcacgtgctc cacgtactgg
     9781 gcgacagacg agcagtggac gaagatcagg tcccacttct ggctggcgag gaggcggtcg
     9841 atcttggcct tcaggtcggg cgaatagaag aagcccatgg atgagggcgt gctcaccggc
     9901 agccgcgcca ccatgcgcag ggcctgcacc gggttgctca cgagacccat ctcgaaggcc
     9961 ttgcaatgag gggcgatgcc ccgcccctcc tccgcctcct ggggcgaacg ggcgagggag
    10021 cagacggtga ccgcatgccc ctgcgccgtg aggtggcgga tcatgttgaa gggccggatc
    10081 ttgccgcccc gcttgggcgg gaagggaaaa cgatggcaga cgtacagaat gttcatcggg
    10141 tgtagctctt cagctcggcc gccacgtcgt cggccgaccg cccgtcgagg tagatccggc
    10201 tccagatttc aagattcatg agggacagca ggccgtccgt gccgtcactg cggttggatt
    10261 cgtggtcggc catcaggccg gcgatgacgt catggcggaa caggccacgg cggcgcacgg
    10321 attccggcga caggagctga cgcagcaccg gcgccagatc gcgcttgagc caggcgccca
    10381 tgggcgtgcc gaagccgcgc ttcttgcggt cgaggatgtc ggccggcagc aagtccgcca
    10441 gggcatcctt catcaggtgc ttgagacgtc cgccgcggat cttcaccgcg gcgggaatcg
    10501 ccgcggcgag ttcaacgagt tcgtgatcca gcagtggaac gcggcattcc agggacacgg
    10561 ccatgctcat cttgtcggtg agcaacagca gatcgtccgg cagctgggtt tcggcgtcca
    10621 ccgcgaacat gcggttgagg tcgtcggcct ggccggcgtc ggagaaggcc tgggccagcg
    10681 gatcgggcgc ctgggcgcct gcatccagca acatggcgga gacggtttcc cgcgccagca
    10741 cctgcaggta gctgcgatac cggtcgtccg gcgccatgtt tgccgaggcg atgaagccct
    10801 tggccaggcg cagggtgttg agcaggccgg aatgccggtc gctgggcagg cgattggcaa
    10861 ctgccgtggc cgcgctgcgc acgaagcccg gcaggcgctg gaaacgctcg gcgtaatggc
    10921 cgcccaggta gcgccgatag ccgccgaaga gctcgtcgcc gccgacgccg gagaggatca
    10981 ccttcacgtc ctggcgggcg aattcggaga ccagaaaggt ggtgatgaag gccgtgtccg
    11041 ccaggggctc gtccatgtgc cagagcagct tgggcagcag cgacaccacg tccggcttga
    11101 cgacgatttc gtggtgctcg gtgccgaaga gctgggagac ctggcgggca taaggcagct
    11161 cgttgtagag cgcctctgcc tcgccgccct cgaagccgat ggcgtaggtg cggatcggcc
    11221 ggtcggagtg gcgcgccatg taacccacca cggcgctgga gtcgacgccg ccggacagga
    11281 aggcgccgat gggcacgtca ctgaccatct gcatgcgcac ggactcttcg aggcgcaccc
    11341 gcacccgctc cagccagtcg gcctcggata ccgtgcgctc tacgacgctg ggcaggcgcc
    11401 agtagcgcca ttcgcggacc tcgttgttct cgatggacag cagggtcgcc gggggcagct
    11461 tgcggatgcc cttgaacatg gagccgggcg cagccgcgta gcccaggtga aggtagctgg
    11521 cgagctggct gcggtcgagc tccggcgaga cgccgggcag ggccagcagg gccttggcct
    11581 cggtggcaaa ggccagacgc cgggcgtcct gcatcacgta gaggggtttg acgccgatgc
    11641 ggtcgcggcc gatcagaagg cgcttgcgcc gccggtccca cagggcgaag tcgaacatgc
    11701 cattgaggcg atggacgaaa tcgtcaccga aggcgtcgta ggcgtggagc acgacctcac
    11761 tgtcggagcc ggtcttgaag tggaagccca ggccctgcag ttcggcgcgc agctcgcgga
    11821 agttgtagat ctcgccgttg cagacgatcc acaggctgtc gtcggcgttg gagatgggct
    11881 ggtggccacc ggccaggtcg atgatggaca gccggcgcat ggcgatcccg cagggaccgt
    11941 cgatgtactg gccttcgtcg tcgggcccgc gatgctgggt ccggtcgccc atggcggtga
    12001 gctgggcggg ttcgacccgc tgaccgtcaa atcggaagaa tccgtgaatg ccgcacacgt
    12061 tgcaacctca gatggaaagg gtacggccgc gggccgtcag ggcccgccgg tagacgtcgc
    12121 gataacgggc cacgctggtg gcccaggtgc gttcgacctc gacgaaactc cgggcctgct
    12181 ggcgcacctg gggccagcgc tcgcgatggg tcagcacgtc gtcgatggct gccgccaggg
    12241 cgtccacgtc gccggcgcga aacaggaagc cagtctcgcc gtggcggatg agctccttgt
    12301 ggccgcccac gtcggaggcc acgaacatgc gtccctgggc catggcctcc agcggcttga
    12361 ggggcgtcac cagctcggtc aggcggatgg gcaggcgcgg ataggcgagc acgtcgatca
    12421 gttcgtaata acgctgcacg accgcatggg gcacgcggcc ggaaaagatg acccggtcgg
    12481 acaagcccag ctcggcgacc agggccttga ggttcgcgtc ctgcggtccg ccgcccacca
    12541 gcagcacgcg caggtccggg tagcgatcca ccagctggcg cgccgcgcgg atgagcaggt
    12601 cgagcccttc gtagccatag aaggagccgg cgaagcccag caccgtcttg ccgtcgaggc
    12661 ccaggctgcg gcgcagttcc gggtccgctt cggcgccgaa ggtgaaggcg gatacgtcta
    12721 cggcgttggg aatcaccgtg atgcgctcgt ccggcacgcc gcgtacggcg atgtcgccgc
    12781 gcaggccttc gcagatggtg gtcacctgat ccgcatggcg cagggcgaag ctttcaagac
    12841 cgcgggacac ccggtagcgc aggctccctt cggcggtggt gccgtgatcc acggcggcgt
    12901 cttcccagga ggcgcgcatc tcgtaaacga ccgggatgcg gtgcttgcgc ccgacccaca
    12961 gggccggcag cgcgttgagt acgggcgaat ggggatggag gatgtcgggt cgcagcgtct
    13021 gcaccagatc gtcaagccgc gcggcggtga ggcgcatctg cgcgaccagg ccttcctgcc
    13081 cggtgttggc ggttcggtgg aaggtccagc cgtcggcctc ttcggcaccg ccggaggaga
    13141 cgccctgctt gggcgtggtg acgtggaagg tttcccagcc gagcttgcgc tgctcccgca
    13201 ggatggacac cgtgcggaag gcgtagccgc tgtgcagcgg gagggaatgg tcaaggacgt
    13261 ggaggacgcg caggctcatg ccgacctcac gagcggcgcg ggctgggccg tggtctgaca
    13321 gttgcgcagg aaggcctcga acatcagggt ggtccagatg gcggagctgt agtcacgagc
    13381 gccggattgg tgggcatcca ccagatggcg caggtaggct tcgttgaaga gcccggtggc
    13441 gcgcagggtc tcgccgagca gcgcctcgcg cacccgctcc ttgagggggc cacggaacca
    13501 gcgcgacagg ggcaccgcga agcccatctt cgggcggtac agcacgtcgc ggggcagcat
    13561 cggctccatg gacttcttga acacgtactt gccttcctgc cccttgatct tgaggctgct
    13621 gggcagggac gcgagccatt ccacgagggg atggtccatc agcggttcgc gcacttccag
    13681 ggaatgcgcc atggaggccc ggtccacctt ggtgttgatg tcgcccacca gataggtctt
    13741 gatgtccagg tactggatca gcgccagggg gtcatcggtc tgggcattgg cggcatggcg
    13801 gcggaacacc tccagggcgt tgtaaccgcc cagttcctga cggaaacgct gggagaagag
    13861 ctgccgacgc atgtcgtccc gcaggatcga cacggaatgg aaataggcct ggaccgaatc
    13921 ccgcgcaagc gcctggaagg tggtcttggc gcggaacacc cgcggcgccc agtcggcctt
    13981 gggatacacg gagccgagga agccgaagag cggtcggcgc aggccgtagg gcagcccgcc
    14041 acgcatcttt tcttccatca tgtggaagcg gtagcggcga tagccgccca tgttctcgtc
    14101 accaccgtcg cccgacaggg ccacggtgac gtgcttgcgg gcgagctggc agacccggta
    14161 ggtgggaatc gcggagctgt cggcgtaggg ctcgtcgtac agcgcggcga gggtgtcgat
    14221 gaggtcgaag tcgtcggact ggacggtctc gacgcgatga ttggtcttgt agcggtccgc
    14281 caccatctgg gcgaattcgg attcgttgaa ggccggatcg tcaaaggcga tggagcaggt
    14341 attcaccggc tcgtccgaca gcccggccat ggtcgccacc accgcgctgg aatccacccc
    14401 gccggacagg aaggcgccca ggggcacctc ggcgatcatg cgcaggcgca cggactcctt
    14461 caggcgctcc ttcagctccg ccgtggcgtc ggcgaggttc aggttgtggt cgagggtgaa
    14521 acgcacgtcc cagtagggct tgggctgggg tattggctcg ccgcggcgga tcgtcagggt
    14581 gaagccggga tcgagcttgc gcgcgccctt gaagatcgtg cgcggctcgg ccacataacc
    14641 cagggcgaag tattcctcca ccgccagcgg gtccatggtc cggtccaggt ccgggtgctg
    14701 catcagcacc ttgagttcgg agccgaagac cagcgtgcca tccctcagga tggcgtaata
    14761 catgggcttc acccccatgc ggtcgcgggc catgaagaag gtctggcggt tgcggtccca
    14821 cagggcaaac gcaaacatgc cgcggaaacg ctgcacgcag gcctcacccc acgattccca
    14881 cgcatggacg atgacttccg tatcgctgcg ggtgtggaag cggtgaccga gggcggtgag
    14941 ttcgggaatc agttcctgat agttgtagat ctcgccgttg aacaccacca ccaccgagcc
    15001 gtcctcattg aagaggggct gctgacccgt ggacaggtcg atgatggaca gacgccggtg
    15061 ggcgaaggcc acccccggct cgaagtgata gccgctttca tccggtccgc gatgggactg
    15121 gatgtcgttc atccgctgca tcagctcgcg cggatagtcc tgctggcccc gcgtgtcgaa
    15181 tattccggca atgccgcaca tagtccctac cttaaattcc gcgttgccgc ttagctcgcg
    15241 ctgtcagcgc cccttggccg cgcacagggc cagatagaga tcggcatacc gcttcaccat
    15301 gccgtccagg ctgaaattcg cctccacgcg cagccttccc gcgtgtcctt ccgcccggcc
    15361 ccgcagcgga tcccgcacgt actccagcag cttatcggca agacgctggc tgtcctgagg
    15421 gggaaccagc gcgccgctct ccccgtcggc gaccagttcg ccgttgccgc ccacgtccgt
    15481 ggccacgacc ggcaagccgg ccgccatggc ctccaggatc gtgttcgaga tgccctcggt
    15541 ctgggagggc agcacgaaca gatcgaaacc actcatcatc cggggtacgt cagaacgctc
    15601 cccggccatc cacacccggc cattcagccc gagcgtccgg atctcatcct cgatccgccc
    15661 acgcagcggg ccgtcgccag ccagcaccag acgcaccgat tcgctgtgcg tcgcatccaa
    15721 ttgacgcatc cgggcgaagg ctcgcaccag attgagctga tccttgaccg cctgcaggcg
    15781 ccccaccgtg ccgatcacga aatacgccgg atcgttgaag ggcgatccct cgagcgccgc
    15841 acgacgcgcg cctcgcggcg tgaatcgcgc gacgtcgacg ccgttgcaga tccgctcggc
    15901 ccgtctgtca ggaatgccga ccgcacgcac cagatatgac aggatctgcc cggacagcgc
    15961 aatgtaatga gtcacgaagg gtctgtagag ccggcgcatc cagcggaact tggcgcgcgt
    16021 gccatgtgga tccgagacat cccagccgtg ctctccatga atgcggacgg gaacacccgc
    16081 cgcccaggcc ggcaccgtcg cctccagcgc tgccagattg cgggtatgca cgacatccgg
    16141 acgatacgcg cggaacagcc tgaacaatgc cggatagatc ttgaccccct gccctggcgg
    16201 tttgtgcagt gcgacgaatt ctacatccgg cacctgcacg cgggcacaga aaagcggttc
    16261 acattgtgtc aatgccacga cgatatgccg aaagcgtgtc cgatccatcc ggttgatcag
    16321 attgacgacg ccattttcta gcccgccgac ggcaaagcta tacaccacgt gcatgacgag
    16381 caatcgcccg tcagggtgaa gcgcgctcat ttcacgcggt ccagcgactg gagctgctgg
    16441 aacagcggcc cctgcaggga cgcggcagca tcctccagcg cctgttcggc ggaacgctcg
    16501 tcgggcgccc gcgaccacat cgccacatag gccgacgtgt cagccttgcc ggtcaaccgg
    16561 gccagcgtca agaggccctt ggcgacgtaa tcgctggtga ccagtcgacc gtcgatccag
    16621 tacgcgctcc agaccacgat gcgcgtcccc tctccgctga ggaccgaacg cttccaggcg
    16681 ccgaagggcg tctccggacc ggccccctcg tcgagcatcg cccaatggtg atcctcgacc
    16741 gacagcagca cattgcctgc gcgaaccaat tcacggccct gggactgatt cgcgtagacc
    16801 gccacatagg cattcaccgt gcgccgcccg tcggaatagc cttcctccat cagcccgcga
    16861 tggccgctga agcgtggctg gaatgcgtgc cccgtttcga caccacccgc tcgccatgcg
    16921 ccaccggccg ccggcaacgc tatcgggatg gtgacatccc gatcagtcgg cgccgcgagc
    16981 aggacggcga ccaccggcca caccgcggtc agcagggcga acggcagcac gccccgcacc
    17041 agcgccgccg tgtcgattga tgcggccggc gcagggaccg ccgcagccgc cggcagctca
    17101 tccagatcct cccgccagaa cgagcccacc cagaacatga gcaggatcac cagcccgaag
    17161 aacacccagc catagatcag gtgatcaaca ccggtggcga gctggttatt gctgaagtag
    17221 cccagcatca cgatcatgaa ggccctcagc cagttggcga ccaccggcat cacgatggcc
    17281 accgccgtga agatcagccg tcgccgcaga ctacggaaac tgagataggc gtagagggtc
    17341 ccgaccatca ccgacgcaat cagataacga aagccgctgc aggcctcaat caccgaccaa
    17401 cggccgttgg gcaggacgaa atgcagccct tcctggtaca cgggcacgcc catgagacgc
    17461 acggccgaca cggtaaacaa agccgtgtac ttcatcatga caggtaacag gaattcacct
    17521 gcggggatgc cgaacagcag aaacagcagc ggaaatttca gggcccccgc ggcttcgcgg
    17581 ccgagcacgc cgaagagcgt cagcaccagc atcgtgacaa ggcagaaatg cgagaccgag
    17641 gccacgctgc ccaactgccc gagcagccat ccaaagccgg ccaccgcgac cggcaacagc
    17701 cagatcggcc acgcgcgcag cggacgggtc gccagcacct cgcgcttgcg ccataccagc
    17761 catgcaaaga tgggaatcac catgaggccg tgggcatagg attccgaacg ccaccatacc
    17821 ccggcgatgt cagccgccgt ggtgaagtag gcgccaatga tccacagaaa cacgccggcg
    17881 atggccaccg gtaccgctcg tcgtgaattc atgccttgtc cgcctcgcac gcggcacgcg
    17941 gcatggaccg ccgccgcgcc tcaatgaacg tcgtcaccat gtttaaccca gattccggac
    18001 gatcatgggg cccaggcgat tggccacccc caccggaagg cgcttccaca gggcaatgaa
    18061 ggcgcggtac ttggggttca tgggattgtt ctggggaatc tcgtcccgct tgaaaagccg
    18121 gtattcgtac gaaaggggca tgggctcgaa gccccagttc ttcttgaaac tgtacgggcc
    18181 cgtgccgatc ttgctgcggc catagtcgaa gaggcggcag ccccgggcgc aagcccgtcg
    18241 catcagttcc cagtatttga agtcgttggc ggccagatcc cgcgcctcca tgtcgtcacc
    18301 ggcgtaatag ggcaggatct cttcacggaa gtagaagctc agcacggagg acaggagctt
    18361 cccgtcggcc gtggtcaccg tcatgacttc gcaatccttg ccgaacacgt ccagcagggt
    18421 ctcgaagaaa cgcttgggca gcgccggcgt accgtggcgc aggacattgt ccgaatacag
    18481 ggcgaagaaa cggtcggcgt tgggatcgac gtgactcacg agtccgttct tgatgccctt
    18541 gcgaaccatg gcgcgctgct tgcgcggaat ggccagcatg ttcgcctcct cgtcgggcag
    18601 gatctccttg cggaaggtga cgtacagatc ctgccgcggc cagtcttcgt gacgggcccg
    18661 caggttgcga tattccaggt gctggacgcc ggcctgtcgg gaaatggtct ccgcctcggc
    18721 ctccagcgca gccaccgcgt cggcgtctga gcctgccacg ccgccataca ctgcaaacgg
    18781 cagcgaggtc atggaatgcc cgaagaggcg gctgttcacc tcaaccagcg gcagcaccgc
    18841 gacgatatgc ccatcccgtt cggccagcag gtaatggcaa cgatggcgga agacccgctc
    18901 gatgatgtcg cgccattcga tgcggtgaaa gaaggtggcg tcggggcagt cgaagacgaa
    18961 tgcgttccag cgcgtggaat cgcccgcctg gaagcggcgt acggaaatcg gtgaagccat
    19021 gtatcggaac ctaagctgac ggcgtgtcgc cggaaaggaa cacctcgtcc atccgtcccc
    19081 acgcaaaatc attgagcaga cgggtgaagc gggcctcggt gcgctccaga ttgacatagt
    19141 ggcggaagcg ggtcttggca ctcgcctcaa ggaccaccgg ttgcccggga tcgagttccc
    19201 agggatggaa gtagaagatc gccggcttac ggtcacctgc attcacctgg cgaatgcccc
    19261 acgccgacac gccataaggc agcaggcgga aatagccgcc gccgcccacc ggccagttcc
    19321 gcccgaaacg ccggaccgtg gtgatgggaa tctcgacgag actgttgccg accgggtacg
    19381 tgaaacgcgg cgcatcgggc acaccgtagt ggtcatggcg caccggatac acacttgaac
    19441 tgtagacgta acccgcggcc tggatgcagt cgtgggccca cgggttgctc agtccgaccg
    19501 aaaagcttgg cgcacggtag ccacgcactt cgaggccggt gatgtcttcg aggatcgcct
    19561 tggccaaccg gatgtcgcac aggaaggctt caggcgactg atcgctggcc cgctcgtgac
    19621 cataaccatg actggcgatc tcgtgaccgg cttccgcaat gcgccggacc agcgccgggt
    19681 agcgctcggc aatccagccc agggtgaaaa acgtgccctt ggcaccggca gcatccatca
    19741 gcgcaaggat gcgatccacg ttggcttcga cgcgacaggg cgtgccatcc cagcccgagc
    19801 gtggaaaatg cggcgcgagc gcggaaacct ggaagtagtc ttccacatcg caggtcagtg
    19861 cattgaccag tgagcgggcg tcgtcgacat gcggattcat cgggattccg tttccttgcc
    19921 tgccgtctgg gacggcttct gtacggcctg gagaagctgt cccagcgcat tcagcgtggt
    19981 ggacagggtc cgctccagca aggccagacg ctcttcaaag cgcgcggtgt cgaagccttc
    20041 gcctgccttc gccagcgtcc gcagggcctc cggctccgcc gccaggcgcg ccagatcgac
    20101 ccccgcaagc cccgcggaca gcgccgctgc gggcgtatcc gcctgcatgg cgggcggcgc
    20161 cacctgctct tcacgcatct cggccaccac ctgctccacg atggcagcgg agattcggcg
    20221 ctgttccgtc aggaatgcgg ccagcaggac gcgatcgcag acagcgttga tgcggcgggg
    20281 aatgccctgg gaacttgcat ggatcgctgc cagcgcgtcc gcgtcgaaag acggcacgcc
    20341 gcgccagccg acgcaattga ggcggtgttc gatataggcc tgggtctcgg ttgcatccag
    20401 cgggccaagg tggaaggacg cgatcacgcg ctggcgcagc tgcagcatct gcggactttg
    20461 catgatggcg cggaattccg gctggccaac gaggaagctc tgcagcagcg catggtcctc
    20521 gagctggaag ttggagagca tgcgcagctc ctcgaccgcc cggggagtca gattctgggc
    20581 ctcatcgacc accagcagcg cacgcttgcc ggctgcagcc accgagacca gataggtttc
    20641 aatggccacc agcagatcgc tcttgctcat ctgccggctc ggaatgccga aggcggcggc
    20701 caccagacgg aggatgtcgt ccgcgtcgat ctgtgtgctg accagattgg ccgccacgat
    20761 cttggacgga tcgagctttt caagcaggct gcgcacgagc agcgttttgc ccgcccctac
    20821 ctcgccggtg atcacgatga agccttcgtt ctggtgcagt ccgtactcca gataggccat
    20881 cgcccgccgg tgaccgcggc tgccatagaa gaagctgggg tccggattga gctggaacgg
    20941 cttgccgcgc aagccaaaat gggattcgta catgagcggt cagaagagga tgatcacgct
    21001 ccccagcagc gcgttttcgg tgtattcggt ggtgccggtg gcccggctgt ggcggtaggt
    21061 cagagagccg ctggaacgac gggtgaagga tgtggagagg cccgccgtcg ctcgccgggt
    21121 cttgttggtg aaggacggcg tcgtggcact gccgtcagcc tgcgtgcgga tcagcgtcat
    21181 gtcggccgtc gcggatgtaa gcggcgtcag gcgatgattc agcatcagac tgccgctgct
    21241 gtcggtcaca cgggtggcgt tgatgaattc cccgccgagc gccgcagtct ggctgaccag
    21301 cgagctgcgc tccgagcggt agccgctgaa catgaacgtc gtccgtcgca gccccacgta
    21361 ggaagccccg aactgcaggc ggcgatcaag gaagaccccg ttgttgagca ccgactggct
    21421 gatgccgatc tgctgatcgg tggacacccc ctgattggtc aggtaagtcc ggaccgcctg
    21481 atcgcgggca accggatccg gatagcgcga tgcgagcagg cccatgagga gatcgtaggc
    21541 cgtcgtgagg gacgttgcgc tgagcgcctg gctggtgctg gtgacgtcct tggagaactt
    21601 tgcattgaag acgaacggac ccgtggagta gtccccgctc atctggtatc cgggcccgaa
    21661 gaagcgatct tcccaggtgg cggacacgcg gccgaacgca gacggcttcc agtcgaaacc
    21721 ggcccccacg atggtggatt gctgggtatt gctgctgttg tagttgttgc cttcaccacc
    21781 gccaatcacc cgcagctgca gctgcggcgt cacccggctc atcagcgtga gccgcgccga
    21841 ctgaagatgc agatcacggc gtccggtctg ggaaacctga ttgtcgctga acgccagcga
    21901 ccagccggac tgcccgaacg cggtttcatc cacgaggctg cccgaccaca tctgctgttg
    21961 catgcgctgg aaggacccgg agctggcgga ggacgagttg atgtcgtagc gcagttccgc
    22021 cacacccgaa ttgtggaaat gaccgcgcag gtacgggctg ataccgacgt tccggacatt
    22081 ggtctggttg gcgcggcccg tcacctgatc gcccggacgc acgccgagca ccgaggcgac
    22141 ttcacgggat tccgatcccc gcacgtccac atacgcaaat ttttcgacag cctcgacact
    22201 tcctgtcccg ttgaacgtca ggaaactggt gttgaggttc gaattgttgg cgtagaagag
    22261 actcgcgacg ctggcgcgca ggttcccctg ggcgcgacct ccccgactgt tcagctccat
    22321 ggacggaatg atgccggtga tgtaatcgga cttagacgac gcattgcccc ccgtcccgct
    22381 cccgcggctc aggtcgacgt tgtcggtgat cgtctcggtc aggcgtagcg agggggagaa
    22441 cgtcacctga tgatcctgag ccagcacgga catgctcgtc agggcaagcg gaatggtcag
    22501 gacgctcatc cggacgacgg tccgggacag agctcgaccg tgaacggggc ttcggcgctt
    22561 cacgcattct ccttctggga ttgcccgtag ccgtagccat agccgtaacc gtatccgtag
    22621 ccataaccgt agccgtaagc accggagccc gcctggagcg ccttgttgag cagcatcagc
    22681 ttcaccgggc aggcctcgat cgtggagatc gcctgcttga ccgcggcctg cgtggtcttg
    22741 ttggactcca ccaccatcac gatctggccc atgtgggtcg ccagcacgcg ggattccgtc
    22801 gtcaccagca gcggcggcga atcgaacacc acgatccggt cggaatatcg gacggccagc
    22861 tcagccagca ggttgttcat ggcgtcggac gccagcagct ccgtcgcgcg cggatggggc
    22921 gtacccgccg gcaacagggt gagcttgtcg atgttggtct tgagcagcac atcaccgagt
    22981 tccacgtcat ccgtggtcag gacgtccatc agacccttct tcggcggcag acccaggatg
    23041 ttcagaatgg atggacggga tacgtcggcg tcgatcagca gcaccgtgtg gtcgagttcc
    23101 atggccatgc tgatggccag attgatggaa ctgaagctct tgccctcgcc gggcagggag
    23161 ctggtgacca tgatgaggtt tgcactgtct accgccgccg cgcccttgcc ctggatattg
    23221 cgcagcagcg gccgcttgat gatccggtat tcgtcaccga tcagggaacg tggcgcattg
    23281 ggcgtcacca cgccgatgga cgcgaggcgt tcgagatcga tctcgacgct gcgggcggtt
    23341 gatgcgcggg ggcgggacga cactgacgct tcgggcgccg cagacggaac cggcaacggc
    23401 acctcggcca ccggggcagc ctctacggtc acggcagcgg tcaccgccgc gatcacgggc
    23461 gcaggctgtt cgaccggcgt gccgaccggc tcgatgcgcc cctcggccga tccggcaacc
    23521 ggtgcggcct cgaccggcgc accggcgctg gcagccgcgg ccgccttctg aagttgttcg
    23581 agccgttcaa cggccttttc gataatgctc atatccgcct ctcacgcgac cttggccagc
    23641 aacaaccaga cggtcatcgc cccgaacagc cccaccaggg cacctactcc gccgaggaac
    23701 acgatgcggc cgcgacgttg cgtgcccatc cgctcggggt ccgcgatcat gctcaccgcg
    23761 ccgagaaccg gcagaccggt gacatcgcgc agaacctgca tgtcggtgaa cacgggacgg
    23821 atctgactca tcatgaagct cacgacagcg cccagcagca acgccgccaa gccggcaccc
    23881 ggcatcagca gcaggcggtt gggggccgcg ggcttgctcg gcaaggtcgg tggatcgatc
    23941 aggcggaagt ccgcgacacc cgaggtcgcc cccatttcca cggccatggt ggccgactcg
    24001 cggcgggcca cgagggcgtc gtagttcttc ttgttgatgt cgtggtcccg gttcagctgc
    24061 gtgtattcgg cctcgagctg cggcaccatg cgcgacgcgg acgacagatt ctgctggcgc
    24121 gcctcgtatt ccgacacgcg ggccttcagg gacgccacat tggcttccgc ctcggctagg
    24181 gacatcttca tctgctggaa caccgggttg ctgttgatcg acccgactgt gctcggcccg
    24241 gccttgcgcc gggactggat ttcctgattc ttctgggctt ccagctgttc gatcaccttg
    24301 cgcgtgccga tcacgtccgg atgttgatcg gtatatttct gcagcaggga atccagattc
    24361 ttcttctggg cctcgatgcg gccatcgagt tcagggactg acaccgatgc gagggcggaa
    24421 ctgctgggat tctccggcag caggacggga tcctcgcctg ccagttgacg cttcagtgcg
    24481 tcacgggaat tctcggcttc gcgcagggcg agacgggcct gattgagctg ttcgttcact
    24541 tcgcccatct tcgagaaata atcccgaccg ccagccccca tggtgctcag attgcgcagt
    24601 ttgaattcct tcaggcggtt ttcggcctcc tcgagcttct gctcgtaggt cttaatctgc
    24661 tcttcgatga acttgcgcgc cgtatcggaa tccttgcgct tgtcgccgag accggactcc
    24721 acgaagatcg agacgaggga ctgcacgaca cgcttggcgc gttccgcgtt ggtgtccttg
    24781 taggccagcg tgtagatgtt gtcacgaccc gcctcgcgga tctcgagggc cttggtcacg
    24841 ccgacgatca gggcctcgcg ctcttccttg gtcttgacgg ccaggtccag gtcggccatg
    24901 cggatcagct tttccacgtt ggggcggctg atcagtgtgc gggaaaggat gttgatctgc
    24961 tgttccacgt tgggctgcac ggcaagcccc gacatcagcg gccgcaggat ggactgggta
    25021 tccacgtaga cccgcgccga agactcgtag cggtcgggta gcagatacac cgtggcggcc
    25081 gcaatcgggc cgacgatcca cgccgccgcg agcccccacc agcggtacag ccacatgccg
    25141 cggaggatga ccgacagctg tcttaagata tcttccatca ggaatttccg gtagcgctcc
    25201 ccggcctgcc ggccagggag tcagacatga gaacgatcag aaccagctct gggggatgat
    25261 cacaacgtcg ccgggcttga cttcggcgtt ggcggacacg tcgccgcgct tgatgaggtc
    25321 acgcagacgc acgccgtatt cggcgttgtt gtcggaggtc cgcaggatct tggcccggtt
    25381 gccgtccgcg aaatcggtca gaccacccac cgcgatcatc acgtcgagga gcgtcatctt
    25441 ctgcttgtac gacaaggtct gcggccgggc cacttcgccc acgacccgca cctgctcggc
    25501 gtaagggccg acaaagtcgg tgacgatgac cgtgacgacc gggtcccgga tgtacttgga
    25561 aagcgccttt tcgatctcgc gggcaagggt gctggagtca cgcccggagg ccggcaggtc
    25621 ttccaccagc ggtgtcgtga tcttgccgtc gggacggacc ggcaccgtca tggacaattc
    25681 ggggttgcgc cagaccacga tgttgacctt gtcaccgggg ccgatcagat agttgtactc
    25741 gggcgtggtg gctgtcgccg gcggaggcgg cagtttggtg gcgcagcccg ttgcaaggac
    25801 caccgccagg gtcaggagac ccgtcgtcac gatgcgtttc atagactact tcccctgtac
    25861 atggccctaa atcggcctgg aatgataacg agcggatatt acggcagaga acagcgcttc
    25921 ccggagaaaa acgtcacgag caacaaaacc tttaatgcac gtgctattac atccgggttg
    25981 ggaagcctat ccagccggca agcgggccat cgcggcccgc ccggggacgc cggctcagaa
    26041 cgaccagcgg gcctgcgtgt aggccctgtc gcgcgtgtcg tacatgccga agagcccggt
    26101 ggattggcca ccgaagctgt ccacgccaaa cacgaccttg aggttgcggc tcgcggacca
    26161 gacgagtttg ggccggaaca tgaagtcggt acgattgaca ctggagacga acagggcttc
    26221 cgcctcccag ttgttggaga gcttggtgtt cagcagcacg ctggcgccgc cttcgatgcg
    26281 atcctgaccg attcgggggt cgaaatccag gtaacgccgg gcgaaatact ggagattcac
    26341 acgcccgtcc cggaatgcgg ggatgtccac cccgacgacg taatccagcg tgttctgggc
    26401 aatcagaccg aagtctacgg tgggatcgag ggtgttgaat tcgcgcccgc gggtctgcac
    26461 cagttcgccc ttcagcacaa aggcgccgag atccttagag accgtgccgc ccgcctgact
    26521 gatgcgcagg tgacgcggct cgaacaccgg agaagtgatg ctgcccagac ggaagaaggt
    26581 ctgcgaaacg tccatgctgc gatagtagaa gccggacaca tcccagcctg cggcaagggt
    26641 ggacagcctc ccgccccagt tggtattgcc cagggaattg gacggtttga cctcccccag
    26701 caccggcacg ccggccggga tcggatagaa ctccgcgcca ggcttgccga tccggtcata
    26761 ggacggcacc gggatccaga tcagctcggc gtgggtatcg ccgaagtagt actcgccccg
    26821 cgccgcccac tgcggaatcc gcaactggtc gaaatcgggc aggtaaaacg tccgcagatc
    26881 ccgcgcagac acgacatcgg cgaagaacag tccgaccatc tcgccccaca cgatgtgctg
    26941 acggccgaga cgcacatcca catcaccgac cgagaagtcc acataggtct cgcgcagatc
    27001 gaagcccagt tgctgatcac gacgcaccgc atccgggtag aacccattgt cgatggcgaa
    27061 caccgcgtcg gcgtccgccc gacccgagat cttccacttc agaccagcgc tgccaccggt
    27121 cgcccccaat tccagacgcg agcgggccat ggaccagtgc tcggggctag cataggtgcg
    27181 cgccagatcg aactgcagca cacccgtcgt gcgtgccccc tcgtccgcca catccaggcc
    27241 cgcatcgtcc ccttcttcat cagcgtggac aggcgtgccg gtgcacaggg ccagtgcggc
    27301 cagaagcgcc gcggcattgc gggcgagcgt cgtcgttttc ataggattca aatctccgga
    27361 tcatcttcgg gcttgcgccg gtcctgcacg aaaatccaac tgccctcacg tttcatgccg
    27421 agagcggtaa tctggccatc ttccatccgg acgagacggt cggcccggtt catgacccgc
    27481 ttgtcgtggg tcgagaagac gaaggttgtg cccgcattct gattgatctc acgcatcagg
    27541 ttgaggatgc tctcgccggt ccgccggtcc aggttggcgg tcggctcatc ggccagcacc
    27601 accctggaat gggtgaccag ggctcgggca atggccaccc gctgacgctg gccgccggag
    27661 agctgattcg gacgatgatc agcatacttg gtcaggccaa ccatgttcag atagaacgca
    27721 acccgctccg cccgctcttc cttggtcagt tcggtcatct gcaggagcgg atattccacg
    27781 ttttcctgag cggacaatac cggaagcagg ttgaacgtct ggaacacgaa acccagggtg
    27841 cgggcccgca catccgccag actgtcgggc gtctgccccg agacgtcgat gccttcgatt
    27901 tcgacgcgcc ccttgctcgg cgtatcgatg caccccatga tattcagaag tgtcgatttg
    27961 ccgctaccgg acggacccgc gatggccatg aacaccccgg gctccaccac gagggacaca
    28021 ccctccaggg cggtgaccgt ctgttcgccg aggagatagg tcttactgac gttttcgaca
    28081 cgaatgatcg acatgtacgc gctgcactat gttgtttgct tgcgaaggat aacaccgtga
    28141 cccctcaaaa tcccctctgg ccccgccgga atctgctctt ccgcctcctg ccggcagccc
    28201 tgctggccct ttccagcctg cccatccatg cggaagacga cgaattcgcg aaaaacctcg
    28261 tcgccaaggc cgatgaagtg cgctttcccc gcgaaggatt ccaggtggac atcatcgtgg
    28321 attcgacgag cccgggcgaa gatccggacc agcacaagta ccggattcta tccaaaggca
    28381 atgagaacac gattgtgcag accctcgagc cggcgtccga tcggggccag gccatgctga
    28441 tgcggggccg cgacctgtgg gtctatctgc ccaatgtctc gcagccggtg cgtctgtccc
    28501 tcgcccagcg tctgacggga caggtcgcca acggcgatct ggcgcgcgcc aacttcgccg
    28561 gtgactatga tccgaagctg ttacggactg acacgctgga tggcgacaag gtgcacgtcc
    28621 tggaactgcg tgccaacgac cgcagcgtca cctacagcaa ggtgctctac tgggtgcgcg
    28681 cctccaacaa ttacccggtc agggccgagt tctactcggc ctccgaccgc ttgatgaaga
    28741 cctgtcgctt cgagaagtac cagaccctgg gcggtcgtgc ccgcccaacc cagctgatca
    28801 tggaggacgc actgcgcaag ggcgacatgt cggtcctgca ttactcgcac atggcgctca
    28861 aggatctgcc ggaccgcatt ttcaccaagc agtacatgga caaactgaat cgggtcgatt
    28921 aacccgatgc gccccttcag gccagcgtga cgggggcttc cccggcgggc ggagcgatgc
    28981 gaggcaggcc ggcgtacagc gcctgccgca atgcctcggc accgcccgcc ggttcgtcga
    29041 gatccagcct gcgcaccacg ccggcaatcc gcaggtcggc gccgtccgca tcgacaccca
    29101 ggatggtcac gccggccggc gcagtgcgcg ccaattcggc ggtcagctcg cgctccacgg
    29161 tggcagttgg ctgccagccc gtcgcaggcg cgtcacacca gcccatgcgc gcgaagcctc
    29221 cgatcagacg tacgcgctcg atgcccatcc ggaagaagcg gaaatccccg agctgcagat
    29281 aatctgccgc ttccggccgg taggacagat aacgttcgcg cgccgcgggc tcgagatcgc
    29341 agggcacgac atgccccatg agggtcgccc gcggctgctc gaggggcgac gtcgaggttt
    29401 ccgccaccag cagactggcc cgtgaatcgg ccagcagatt gcgggtgtgt tcggccagag
    29461 cactgatcag gaagagcggc gcgccaccac catccagggc gtaaggcaac aaggtgacaa
    29521 aagggtagcc cggcatcgtc accgaccggg ttccaagcgc gccctgattg caggaccgca
    29581 ggagtgcccg ggcttcccgc ggatcgactt tagtcacgcc cacctcaggc gccccgctgg
    29641 cggcgtgtct cgaacagaca gatcccgctc gccaccgaca cgttcaggct ttcgacggag
    29701 ccgtacatgg gaatcctcac cagctcgtcg caggtttcgc gcgtgagccg acgcagaccg
    29761 tctccttcag cgcccagtac ccaggccacg gacgccttct gctcaacctc gtaaagggat
    29821 ttgtccgcct cgcccgccgt accaagcacc cacacgccgg cttcctgcat ctcccgcaag
    29881 gcgcgggcca gattggtgac ggtgatgtaa ggcacggtgt ccgccgcacc gctcgccacc
    29941 ttgacggccg tggcgttgag gccgaccgcc cggtctttgg gcgcaatgac cgcgtgggca
    30001 ccggcagcgt ccgccacccg caggcaggca cccagattat ggggatcctg gatgccgtcc
    30061 aggaccagca ggagggctgc gccgtcgagg ctgtcgagca ggtccggcag catgacctcc
    30121 cgccgccggc catcgatgcg cgcgacaacg ccctgatgac ggcgcgtgcc gaccatcttg
    30181 tcgagacgtt cgccttcgga gggaatcagg cgcacaccca gttgctcggc ctgacggagg
    30241 aagtcccgcg cgcggccgtc cgaacgctgg gcgtcgatgt agatttcgaa aaccgcttcc
    30301 ggatcgtgac gcagcttggc caggaccgcg tgaaaaccat agatgagacg ggaactggcg
    30361 gattcagaca cgggatttcg ctttcttctt ggtgccgccg gctgcgcgct tggacgggcc
    30421 ggtggaggtg gaagcccgtg atccggcagg cttggaggac gtcttggtcg gcttggaggg
    30481 tttcgatgcc ttggcggcct tggacggttt ggcggccttg gacgtttgca ccgacttggc
    30541 aggcttgacc ggcgaaccgc ccgcatcccc ggtcttcgag gccgaccgag cccgacgcgg
    30601 cgcggtctgg accggtgact ccggggacac gatcggggcc atgacctcgg cacccgcagt
    30661 cccattggcc acacgccggc gccgggctgg cgcgggcgtc tcggcggcgg catcaccgcg
    30721 ctcggcctcg ggctgccggg ctggcagtgg gccttcgagc aggcggaagt cgatcttccc
    30781 ggcttccatg ttcacccgca ccagttgcac gcgcacccgg tccgacagtc gataacgctt
    30841 gccggtccgc tcgccgacca gggtgtgatg ggcatcgtcg aagtgatagt agtcgttacc
    30901 caggtcagag atgtgcacca ggccttcgac gaacacgtca tccagggcga cgaagaggcc
    30961 gaagggcacg acggcggaca cactgccgat gaactcctcg ccgatgcggt cacgcatgta
    31021 gtagcacttg agccagttct ccacgtcccg gtcggcctcg tcggcccgtc gctcggtcat
    31081 ggagcaatgc aggccgatgg cctcccactc gcccgattcc tgcggcggct gatagcgttc
    31141 gccggtgagc accgccttga tggcacggtg gaccaacagg tcagggtagc gccggatcgg
    31201 tgacgtgaaa tgggtgtagg cctcgtaggc caacccgaag tgccccagat tgtccgggct
    31261 gtaaacggcc tggcgcaggg agcgcagcat gaccgtctgg agcagttgca ggtccggccg
    31321 gccctgcagg ctggccagca gccgcgcgta atcgcccgcg tggggatcgt cgccgccggg
    31381 gagctgaagt ccgaaggatc ccaggaattc ccgcaatttc acgagctttt cgggcgtcgg
    31441 gccttcatgg acccggtaca gcgccggatg ctcgtggcgc tgcaggaact ccgacgcgca
    31501 cacgttggcg gccagcatgc actcttcgat caggcgatgg gcatcgttgc ggctttcggg
    31561 cacgatcttc tcgatcttgc cttgatcatc gaagatcatc cgcgtttcct gcgtttcaaa
    31621 atcgatcgca ccccgcttgg cccgcgcctt gaggagcacc cggaacagct tgtccaggtt
    31681 ctgcagatgg ggcagcaagg caccgacggt ctccagcgcc gccggatcct gatcgtaaag
    31741 ggcagccgcc acctgggtgt aggtgagccg cgcgtgggag aacatcaccg ccggatagaa
    31801 gcggaactgc ttgatcgtgc cggtctgggt gatggccatg tcgcacacca tgcacaggcg
    31861 ctccacctgc gggttgagcg aacacaggcc gttggagagc ttctccggca gcatcgggat
    31921 gacccgccgc gggaaataca ccgagttgcc ccgctcgtaa gccgaatcgt ccagggccga
    31981 gccggggcgc acgtagtggg agacatccgc gatcgccacc accagccgga aaccccgtcc
    32041 ctggcgctcg caatagacgg cgtcgtcaaa gtcacgggcg gtttcgccgt cgatggtcac
    32101 cagcggcagg ccggtgatgt cctcgcggcc cttccagtcg gtcttgcgca ccttgtccgg
    32161 caggcgccgg gtctgctcct tggcggcgga cgggaactcg aaaggcaact cgtgcttgcg
    32221 cagggcgatc tcgatttcca tgccgggatc ggcgtaattg cccagcacct cgacgacgcg
    32281 cccgacaggc tgggcgtagc gggtcggctg ctggaccagt tccaccacca cgacctgacc
    32341 ggccgtcacc ttggccttgc cagcggaggc gagcaggatt tcctgggcga tgcgacggtt
    32401 ttccggcacc accagcgtga ccccgttctc cacaagcacg cgcccgacga tgcgggtgtt
    32461 ggcccgctcg agcacctcga cgagcttgcc ttcgggccgc ccgcgccggt cggtgccagc
    32521 aatgcgcaca agggcccgat caccatgcag cacctcgcgc atctcgttgg ggccgaggaa
    32581 gatgtcctgc ccttccccgt ccggcactag aaaaccgtag ccgtccggat ggccctggac
    32641 cgtcccacgg atcagatccg ccttgttcgg cagcaggtag gcattgcgcc ggttgcgcac
    32701 cagctgacca tcccgctcca tggcgaagag acggcgttcg aagaattcgc gctcatggga
    32761 cgcgatgtcc atggcatcga cgaggtcagc caggctcagg ggcacgccgc ggtcggcgag
    32821 cacctgttcg atgaactccc gactcgggag gggaaattcg taagttgcca cttcccgctc
    32881 gtaaaacggg tcgctgcggc ggaatgcact cagttttttg ggtgtcttgg gtttaggcat
    32941 tgacagggat gattgctctt ctataatggc gtcttcgttg cccagatggc gaaattggta
    33001 gacgcgctag tttcaggtac tagtgccgcg aggcgtggag gttcgagtcc tcttctgggc
    33061 accagagata ttcaagagag agccggtcga ttcgaccggc tttttctttg tgtgtgttga
    33121 caagcagggg cgcgttctga aggacaggaa acagcgcaaa aggattgcag gatcgacaag
    33181 ccggaccgcg gagagttttc cgcgtgccgg ctttttttca ttgtggtcgt cgtcgatctc
    33241 aggcgccgaa gggatggcgc ttgaggatcg tctcatcccg ctccggaccc gtggagatca
    33301 cgtcgatgga gacttcgcag atttcctcga tgcggtgcag ataggcccgc gcttcaggcg
    33361 gcagcgcatc ccaggtacgg gcaccgaagg tgctgccagt ccagcccggc atggtctcga
    33421 gaatgggttc gcagcgaacg acctcatccg cccccatggg aagcaggtca acgcgcttgc
    33481 cgtcaagcat gtagccggta cacagcttga gctcggtgag gccgtcgagc acgtcgagct
    33541 tggtgatgca caggccggac acgccgttga tgatgattga acgcttcaga gccgccagat
    33601 cgagccagcc acagcgccgc ttgcgcccgg tgaccgtacc gaattcccga ccgaccgtgg
    33661 acatctggta gcccggcgta cccggcgtct cgaaatccag ttcggtgggg aacggtccgc
    33721 cgccgacacg ggtgcagtag gccttggtga tgcccagcac gtagtgcagc cggccgggac
    33781 cgacaccgga accggcagcg gcctgccccg ccacgcaatt gctggacgtg acgaaggggt
    33841 acgtcccatg atcgatgtcc agcagcgtgc cctgagcgcc ttcgaacagc aggttgcccc
    33901 ccgccttgtt gagggcgtag agatcggctg acacgtcgca gaccatgggt ttgatctctt
    33961 ccgcgtcggc catggcctgg tcgtagaccg actggaaatc caccggatcg gcgcccaggt
    34021 attgggtcag cacgaagttg tggtactcca gcagttcctt gagcttctcg gcgaaacggg
    34081 ccgggttgaa caggtcgtag acccgcagcg cccgccgtgc caccttgtct tcgtaggtcg
    34141 ggccgatgcc cttgccggtg gtgccgatct tatcgccagc actcttgcgg gcttcccgcg
    34201 cccgatccac cgccgcgtga taccccagga tgatcggaca gcccgggctg atgcgcagcc
    34261 gctcacgcac cttgatgcca ccagactcca ggccgcgcac ctcggacagc aggtgatgga
    34321 tatcgagcac cacaccgttc ccgatgaagc acttcacgcc ctcgcggacg atgcccgacg
    34381 gcaccagatt gagcttgtat tcattgctgc ccaccaccag cgtatggccc gcattatggc
    34441 cgccctgaaa gcggaccacg ccttcggcct gatcggtcag ccagtcgacg atcttgccct
    34501 tgccctcgtc gccccactgg gtgcccacca ctacaacgtt ctttgccatg cgttcattct
    34561 ccttgaagcg gttcaacctg ccaggcaccg ccccgcagaa ccagtcgccg gtcacagccg
    34621 gcctcacgcc acgtgccgtc atggccgggc aattcaatca cgacgatctc gcccgacgca
    34681 cgcagccggg cgacctcctg ggcaaacccc ggcaccgagc actcgccggc gaggatcatg
    34741 ccccgccctg ccccgtcggg cgtctgggcc gccagttcgc gcagatcgag gctgaagccc
    34801 gtggccggcc gaccacgccc gaaggccgtc ccgacccggt cgtagcgccc gccctgggcg
    34861 agggccaccg gcgaggtgcc gccgtacgcg gcaaacatca cgccgctgtg gtagtggtaa
    34921 ccgcgcaggt ccgccagatc gaagctgatg ggtagatcgg cgagaagtcg cgacagcgac
    34981 tccagatcat ccagtgcgcc ccgaacatcc gcatcatcca acccgctcaa aaccccacgg
    35041 gcacgctcga gcacttcggg cccgccatac agggtcggca gcaccagcag cgcggagcgg
    35101 accgcctccg gcgcgtcacc aagaagatca cgcaagccgg gaatgtcctt ggactgcaaa
    35161 caatcgaaca acgattcctg ctgctcgtcc cgcagccctg cccgcgccgc cagggcacgg
    35221 aacagtccca catgaccgag atcgatgcgt gacgtggtga cgcccgcaat ctgcaaggac
    35281 tcggccagca gccggatcac ttcagcatcc gcctcgaggc cggcatgacc gtaaagctcg
    35341 gcccccaact gaaggggctc gcgggtcgcc gtcaacgaag acggcaaggc atgggccaca
    35401 ctgccgcaat agcacagccg tgcaacgccc tgccggttga gcaggtgcgc gtcgatgcgt
    35461 gccacctgcg gcgtgatgtc agcccgcacg cccatggtcc ggccggagag ctggtccacg
    35521 agcttgagcg tcttgagctg cagatcctga ccggcaccgg tcagcaggga ttcaagatat
    35581 tcgagcagcg gcggcatcac gagctggtag ccattgagcc ggaatgcatc gagcagacgg
    35641 caacgcagac tttccagctt ttcggcctct gccggcagga cgtcctggat atgttcgggt
    35701 agaacccaac gcaacatggg catggtgtct caggaaaaaa cgatcagtac gatcaggccg
    35761 acgagcatgg agctgagtcc gatgaaacgg atctgcccgt cggacatgcg cacgagacgg
    35821 gtgaaggtct cgcgccacat cttgggcgcc acaaagggca acaggccttc aagcaccagc
    35881 atcagtgcga aagccagaaa cagggaggat cccattattt tgcgctcttg ccgcccttgt
    35941 tcgcgtccgc cggtgcggtc gccgtgcctc ggccgccggc actcttcatg tacttgaaga
    36001 aatcggcgtt gggctcgagc acgagcacgt cggacttgct cttgaagctg ttccggtagg
    36061 catcgagact gcggtagaag gcaaagaact ccggattctg gctgaaagcc tgggcgtaga
    36121 tcgcggatgc cttggcatcg ccttcgccct tgaccttctg ggcttcgcga taggcctcgg
    36181 cgacgatgac ttcgcgctgc ttgtcggcgt cggcacggat cttttcggcg tcggcggcgc
    36241 cctgggagcg cagttcgttg gccacccgct tgcgttcggc ttccatgcgg cgatagacgg
    36301 actcggacac ttcgaggggc agatcgacgc gcttcaggcg cacatccaca atctgcacgc
    36361 cgatcttgcg ggcatcctgg tcggccttga cgcgcatctc gtccatgatc ttctcgcgct
    36421 cgccggagac gacctcatga accgtacgct taccgaactc ttcacgcagg cccgcattca
    36481 cggtctgctc aagacgggtc ttggcccgcg actcatcacc gccgacggaa atgtagtaga
    36541 gcttgggatc gacgatccgc cacttgacga aaagatccac cagcacgttc ttcttttccg
    36601 aggtgatgaa gcgctccggc tcattggaat ccatcgtcag gatgcgccgg tcgaaatagc
    36661 gcacgttctg gatcaaggga atcttcacgt gcagaccggg ctccgtgagc tggcccttga
    36721 cctcgccaag ctggaacacc agggcgtact gacgctggtc aacggagaag agcgacatgg
    36781 acatcagcgc caccacgaag agcagaacgc cgatgaaaaa gggtgcgcgg tcacgcatta
    36841 gcggtctccc ctctcgcggt tacgcatcgc ctcgcgcgtg cgggattcgc cggacagcgc
    36901 gtcgggccgt ggcggtgccg ggctattgtc tgcggcaacg gcagcggcag gcacctgggg
    36961 acgcgtctgg gcggcagcct ccggtgttac cgcgccggac gcctgagccg cggtggcggc
    37021 aatcagcttg tcgaggggca gataaagcag attcccgctc cctttgctgt cgatcaccac
    37081 cttcgaactg ttggacatga tctgctgcat tgtttccagg tacatgcgct gacgcgtgac
    37141 ctccggcgcc ttggcgtact cggtgtagat ctggcggaag cgcgacgcat cgccctcggc
    37201 ctgcgcgacc acccgcccct tgtacgcatc cgcctcttcg agcagacggg acgccgtgcc
    37261 gcgggcacgg ggaatgacat cgttggcgta ggcctgcccc tcattcttct ggcgctcccg
    37321 gtcctgcccc gccttcaccg cgtcgtcgaa cgctgcctgc acctgctccg gtggctgggc
    37381 attctgcatg gtgaccttgc tgatctggat gcccgtggcg tagcggtcga ggatgtcctg
    37441 catgagcttg tgggcattgg cggccacctg ctcacgacct tcatagagga cgaagtccat
    37501 cttgctcttg ccgaccacct cacgcaccgc agtctcggcg gcctggatca cggaatcgtc
    37561 ggcgttgcgg ttgttgaaga gatattgctg tggatccttg aggatgtact gcaccgcgaa
    37621 ctggatattc acgatgtttt cgtcatccgt gagcatcagg gcttccttga gcaccttgtt
    37681 gcgctctgaa ccgcgatagc cgatctcgac ggtgcgcaca cccgtctggt tcacgagttc
    37741 atgggtctgg accgggtatg gcagccgcca gcgcaggccc ggctccgtcg cctcgacgaa
    37801 gcgcccgaat tgcaggacga tgccgcgctg gctggcgtct acgatgtaga aaccgctggc
    37861 cagccagatc accaccgcca acgccaggat caggccaacg cccccgccga actggcggaa
    37921 attcagttcg ggcatccggt cgccgccccc cgaatcaccg ccgccgtcac cgccacggcc
    37981 gggctggcgc ccgaacattc cggacagtcg gcgattgaag tcccgccaca actcttccag
    38041 atcaggcggc ccctgattgt caccgccacc gcggttgccc cacttcgggt cgttgataga
    38101 catgaagatg cccgtgatca ctgtgaaagt ccgctccggg aagaaagccg ggattcagcc
    38161 cagttgaggc tgatccgaag gtgcggcgag tgccgccttg tgagccagtg caacgtcggc
    38221 caggatctgg cgcaacagat ccagaccctc gcctgtcctt gcgctcagca gaacccgcga
    38281 gattttacca taagcgtcca cctggacctc cggactggcc agggtggcat cgatcttgtt
    38341 ccacaccagg atctgcggga tgtcgcctgc accgatctcg tgcagcacgc cattgaccgc
    38401 atcgatctgc ccgtcccggt cgtcgctggc cgaatccacc acgtgcagga gcagatccgc
    38461 cgcgacggtc tcttccagcg ttgcatggaa tgccgccacc aatgcgtggg gcagatcgcg
    38521 gatgaagccg acggtatccg acagcacgat cgtaccggcc tcgtccccca ggtacaggcg
    38581 ccgactggtc gtatcgaggg tcgcgaacaa ctggtcggcc gcataggcac cggccttggt
    38641 cagggcgttg aacaaggtgg acttgcccgc gttggtatag ccgaccaggg atacggacag
    38701 cacatcccga cgctcccgtg cccgccgtcg cactgcgcgc tgacgctcca gcttgcgcag
    38761 acgatccttg aggagcttga cccgctcgcc aagcaggcgc cggtccgtct cgagctgttt
    38821 ttcgccggga ccgcgcaggc cgatcccgcc cttttgccgt tccaggtggg tccatccccg
    38881 cacgagacgg gttgccatgt gctcgagctg ggccagttcc acctgcagct tgccttcatg
    38941 actcttggcg cgcagggcga agatgtcaag gatcaacgcg gagcgatcca cgacccggca
    39001 attcaggcgc ttttccagat tgcgctgctg ggccgcggag atgtcgtggt tgaagatgac
    39061 aagatcggcc tcgttcatgc gggccagcag gccgatttca tcggccttgc ccgttccgac
    39121 gaagtgcgcc ggatcgggcg cctgacgccg gccctgcacg acccctacga cgcttgcgcc
    39181 ggcgctggag gccagcaggc tgacttcggc gaggcgctcg tcaatacccc cctgcccgaa
    39241 atccacctgg acgatgatgg cgcgattgcc cgactcgggg cgatcaaaca tgacggacgc
    39301 cctggaaacg gagttgccga tcagccgtga tacgcgccct gaggtgagaa ggtttcagac
    39361 tcagcttgca gcctcgttct gttcctgctg gatgttcacc ggacgggctg gcaccaccgt
    39421 ggaaatggcg tgcttgtaca ccatctgggt cacggtgttc ttgagaagga caacatactg
    39481 gtcgaaggat tcgacctgac cctggagctt gatgccattc accaggtaga tcgaaacagg
    39541 cacatgctca cgccgcaggg cgttgaggaa tggatcctgg agaagttgtc ccttgttgct
    39601 catagtgtga acctcttggt ttttagtttg aaataattat aaagccattt gctgcaccgc
    39661 cgcatgacga ccgctctggc ttggtgtggt gtggaactcg gtgcgttcaa tcacgatccg
    39721 cgtagggatt gtgagacgac ttgaactgga tgcgcaacgg ggtcccctgc agtttgaacg
    39781 cttcctggaa gcttcgttcc agatagcgga cgtaggacgc cggcacatga tccagcgcat
    39841 tgccgtgaat gacgatgatc ggaggattca tgccgccttg gtgagcataa cgcatcttcg
    39901 gccggaagcc cccgtggcgc gggggctgct gcttgacgat ggcatcgagc aggaggcgcg
    39961 tgagcttggg cgttgcaagc ttggtcatgg ctgcggcata cgcgccatcc accgaccgga
    40021 gcaggggcgg aatcccctgc cccttcagcg cagagatgta cagaaaccgg gcaaatccca
    40081 ggaaaggcag cttgcgggca atgtcctgct tgatgcgttc gcgacggtag tcgtccacgt
    40141 catcccattt atttactgcc accaccagcg cgcgccccgc ctccacaacg aatccgccga
    40201 tgtgggcgtc ctggtcggag atctcctggc tcgcgtcgag tacgaggacc gccacattgc
    40261 attgctcgac cgcctgcagg gtcttgatga ccgagaactt ctccacggct tcgaaaacct
    40321 tgccacgccg gcgcaggccg gccgtgtcga tcagcgtgta gtgcttgccg ttgcgctcga
    40381 aaggcacctc gatggcgtcg cgggtggtgc cgggcatgtc gaaggcgatg acccgctcct
    40441 cacccagcag cgcattgacc agcgtggact tgcccacgtt gggccgtccg acgatggcca
    40501 ctcgggggcc ctgggtcggc gcctcgtcct cgccgccatc ggacgggaag gtgtccacca
    40561 ccagttccat cagcgccttg acgctgtcgc cgtgggccgc agagatgcaa tagggctcgc
    40621 ccaggccgag agcgtgaaaa tccagcgaga ccatcgcccg gttcatgcct tccgccttgt
    40681 tcaccaccag aaaggtgggg cggccggcgc ggcgcaggcg aatggcgatc tgttcgtcgt
    40741 gaggcgtcag cccgctgcgc gcatccacca tgaagagcag cgcgtcggct tcggcgatgg
    40801 cctgctcggc ctgcttggcc atctcatgaa cgatgccgtc cttggcaacg ggatcgaagc
    40861 cggcggtatc caccaccagg aactcacgct cgtccacgcg accgaggccg tagcgacgat
    40921 cccgcgtcag gccgggaaaa tcggcgacca gcgcgtcgcg ggtcttggtc agacggttga
    40981 aaagggtgga cttgcccaca ttggggcgcc ccaccagcac cagcgtcggt ttcacgtcgt
    41041 cgccactcct actgcacgcc aaaggcgaag acgctgccgt cgcgggtctg caccagcaga
    41101 tcgctgccca cacggcgcag atccgccagc accggactgc catcggtacg ctgacgaccg
    41161 acgaaggtgc catcgtcctt gcgcagcacg tgcacgatgc catctacgtc ggccaccgcc
    41221 accatgtcgc ggaaagccag cgggcggccc ggactgcgtc cggcgagctg gtcctgcttc
    41281 cacatgcttg caccgctggt gaggtcgagg gcatggacgg cgcccttgtc gtccgtcacg
    41341 aagacatggc tctcgtcgag atcgagaccg cgcgcgctgg aaagatcacg ggtccacaag
    41401 gtcgaaccgt tggccagatc gaagcaggcc acgcggccct ggtaggccac cccacaggca
    41461 gcgcggccgt tcaggaccgg cgggctggag atgtcggcca cgcgctccag ttcggtcgca
    41521 ccacgaggca ccgacaccgt cccttcccac atgagcgcac cggtgttgac gttgacggcc
    41581 gcgagcttgc cgccgggaaa cccggcgaag atcgcgcgat ccgccatcac cacgcctgcc
    41641 gccgaccgca gcgcaagggc cggtgtcgcg cgctgataga cccagcggcg cttgccgtcc
    41701 gcaggctcca gtccgaacag gcggctgtcc gccgcacgca cgaccacgac accgtccccc
    41761 agggcgggcg cggccagcac ttccgcattc acgttggcct gccagcgtat tgcaccgctg
    41821 gccggatcca gtgcgtaaac ctctcccttc gggctggcca ccagcaccat ctcggcgctt
    41881 ccgccaacac cgcccgacag cccggtcttc agcgacacct tccacacggt gcggccatct
    41941 tccacgcgca ccacggtccc gtcggcagcc gcggcataaa ctgcggagcc cacaaccagc
    42001 ggcgagaaca ccgacgtacc cgcgcttccg acccgcagct tccagtcttc accaagcttg
    42061 acgctgtcgg tgatcggctt gagcggcggg atcttgggtc cgctgctcga aaaaggattc
    42121 agcgaactgc agcccaacag gccgaacgcc gcgatcaggg ccgcgacacg cccgaggcgc
    42181 ctcactgggc acctcccaga ccgtccagtt tggcctggac cacttccttg aacgcgacgg
    42241 cttcaggctt ggtagccgag gccagcgcct cgagcgcagc cttgtaggcc ccacgggcat
    42301 cggccacctt gccctgagcc gccaggatgt caccgcgcag atccccgtag cgggcctcca
    42361 gcgcctcgga gggcttggcc tgcaactggg ccagcgcctc gtcataggac ttctcgtcga
    42421 gcaacacggc cgccagacgc agacgggcca attcacgcag cgcaggatcg ttggccttct
    42481 cggccgccca gccaagctgc gcacgggcgc tcttcaggtc accggcatcg gcctggaccc
    42541 gcgaagacag cagcgccccc agatcggcgt aggccgtacc gctgtatttc tcgatgagca
    42601 tgccggcggc ttctcgggcc tgctgcggct tgttctcggc cgcggccttc tgcaccgccg
    42661 cgtacagcac cgacgcctca ccgctctgct taccctggta ccagttccag ccctgccagc
    42721 cgacggccgc cacggccgcc gccagcgcaa tgcccgtcac caggttgcca tacgtgtccc
    42781 accaggcctt gatctgggag atctgctcct gctcttcgag gtcataggcc gccatcgtcg
    42841 tcctccagtt caagcaggcg atctgccagg gtttcgggaa gaatttccat tggaattcgg
    42901 atctgttcac cgccactgcg caggggcttg aggctcacct cgcccgctgc ggcttcgtcg
    42961 tcaccgatca ccacggcgta ggtggcaccg gaggcatcgg cgcgcttcat ctgggacttg
    43021 aaactgcctc cgccgcaatg cagggtcacc gtgaagcccg tcccacgcag ggcttcggcg
    43081 gcgcggaagg cggcacgctg ggccgcttca ccggcatgca cgagatacac gtcgggcgtc
    43141 aggggctcgg gaatgaaacc gctctcctgc cacagcacca gcaggcgctc gatgcccatc
    43201 gcaaacccgc aggccggcgc tggcttgccg cccagctggg cgatcagccc gtcgtatcga
    43261 ccgcctgcgc agatcgtgcc ttgcgcaccc aggcgggtcg tgacccactc gaacacggtg
    43321 cggttgtagt aatccaggcc gcgcaccagc cgatggttga tgcgataggg gatgcccgca
    43381 tccttgagca gggcctgcac gccatcgaaa tggcgcaggg attcgtcgcc caggaagtcc
    43441 atcagcctgg gggccgcttc gacgatgtcc tggaggtccg gatttttggt atcgaggata
    43501 cgcagcgggt tggtatggag ccgacgctgg ccgtcctcgt cgagccgttc cttgtagcct
    43561 tcgagatagg caatgagggc ggcccggtgg gcggcccgct cgtcggcgct gcccagggag
    43621 ttgagctcaa gcttgacgtc ttcgaggccc aaatcgtccc acaggcgcgc gcacatgaga
    43681 atgtgctcgg cgtcgatgtc cgggccatcg aagcccagcg cctcgacacc tacctgatgg
    43741 aactggcggt aacgcccctt ctggggccgc tcgtggcgga acatggggcc gtggtagtac
    43801 agccggcgcg gctggccgaa gatcagactg tgctggatcg ccgcccgcac acaggaggcg
    43861 gtgccttcgg ggcgcagggt caactgttcg ccgttgaggg cgtccacgaa ggagtacatc
    43921 tccttctcga cgatgtcagt gacttcgccg atcgcgcgct tgaacagtgg cgtcggctcg
    43981 acgatgggca tgcggatggg acggtaaccg tagctgcgca gccagtcgcg gacgatgtcc
    44041 tcgaactgct cccagacttc ggcatcgtcg ggcaggatgt cgttcatccc gcgcacggcc
    44101 tggagggtct tgctcataaa gtgcagtctg cgtcgcgacg gtcaggccgc cgcgcctttc
    44161 ttgggataag tgcgggccac gtaacgctcc acgatggcct tgaattcgcc ggcgatgttg
    44221 tcgccgcgca gggtcagggt cttgaccccg tcctcgaaca ccggcgccgc cgggctctcg
    44281 cc
//
LOCUS       DAOQPA010000010        44274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_333_length_44274_cov_18.316832, whole genome shotgun sequence.
ACCESSION   DAOQPA010000010 DAOQPA010000000
VERSION     DAOQPA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 44274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44274
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_44274_cov_18.316832"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /locus_tag="PKX01_02710"
     CDS             complement(<1..2150)
                     /locus_tag="PKX01_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ75062.1"
                     /translation="MHDNEAHARVAINAQLADQGWDLHNPNSVCYEVALDDGIADYVL
                     NDRHGRALAVTEAKRFTVDPATARDQAGRYARQLGVAYVFLANGHEILFWDWQREAHP
                     RPVKTFFGQDDLERRAAVRQIRIPLDRVPIDTRIAGRDYQLACIDTLSAEIARGRRKL
                     LVEMATGTGKTRTAAALIKRLFEAHAITRVLFLVDRIPLATQAEDAFAEHLPDFPAYV
                     LRAGRRFQDEKRITVTTLQSMVNLYRDYSSGYFDLVVTDECHRSIYGKWSGVLRHFDG
                     LQIGLTATPCVAPPGDERAPDPDDEDRLFVRDTLRFFEVEQPTFSYGLRQAIADGHLV
                     PYQIYRARTVRTATEGGFTVTRGELDWNALDADTRTELTQLFGPDDTLTVDPNALERR
                     FTIPARNQAIVTEFRKVMDEGFRDHKGILRKPLLGKTIVFAVTKRHAATLAKLFDDAF
                     ADCKPDPAVAYADYVVSGCGDDDTTDGLTKIQRFKRQEFPRILVSVNMLDTGFDCADV
                     ANLVFARFTRSTILYQQMRGRGTRRAPNKPVFTLFDFVGVVDFHGDADTYGEGGLVRE
                     TPARRRYSPRRLLGLPIDDTIDPASREWITLDADGNLVFPAASERRAAQLGTRFEAWM
                     LHNADLSAEAERWLHLLGNQIRANADTLDEITPEHFAYFHVFAQMGGLAEARRVLGGD
                     ARLAALLGALNAAVFGPLDDASADPAPGPDDAPRP"
     gene            complement(2238..2429)
                     /locus_tag="PKX01_02715"
     CDS             complement(2238..2429)
                     /locus_tag="PKX01_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75063.1"
                     /translation="MEDPVLYRFCVRAKASPDQPEASRPAFITPYPLTEADAAERFEV
                     LECLQHTAQPLSSVSYVFD"
     gene            2558..2908
                     /locus_tag="PKX01_02720"
     CDS             2558..2908
                     /locus_tag="PKX01_02720"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HMZ75064.1"
                     /translation="MLHYAVKVAVSAGLIVAISEIARRSSGVAALLAALPLTSVLAFV
                     WLYVEGQPTEAIASLSGQIFWLVLPSLVLFVALPWLLRQGVGFWAALALAMGLTAGAY
                     AVLRPLLRHLGVDL"
     gene            3161..3862
                     /locus_tag="PKX01_02725"
     CDS             3161..3862
                     /locus_tag="PKX01_02725"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF009732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HMZ75065.1"
                     /translation="MAHPDHLLWRQGWNANRIPFHLTHIHPLLRRFWDTLDPAATARV
                     FVPLCGKSLDLMWLHGLGHDVVGIELSAVAVGDFFKAARLRPQRTRQADLTCWTQERL
                     AIFCGDFFDLTEEDLQGVSAVYDRAALTALPEGLRADYVAHLHAILPAGCQVLLMTVE
                     DLDDDEAAVEHMASSDEVTALYAGLFSVELLHAEYHEAVQGRGDGVVDGRCIHKVYRM
                     RREARSPSGTGGTVA"
     gene            4068..4973
                     /locus_tag="PKX01_02730"
     CDS             4068..4973
                     /locus_tag="PKX01_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ75066.1"
                     /translation="MIRLRLSVELSYDVAPPGCDVIFNVHAAHTSHQRVATEMMEISQ
                     FLLPQVHTDVATGNRYMRVKAEAGGLRLRYSATVEVRHHRAVPDGIHEVPVSQLPTGA
                     LIYLYPSRYCQSDRLYDMARQTFGHLLPGYARVQAICDWVRAHVKFQSATSTASTSAL
                     DTLRDKVGVCRDFAHLMIALCRALNIPARFATGIDYGADATLGPHDFHAYVEVFLGDR
                     WYLFDPSGTAIPMGLMRFGTGRDAADVSFATLFGTVNAAPPRIDINAIEDAATGALLP
                     WRCPDALSTDDGIGMLVGTRLVSAV"
     gene            5022..5543
                     /locus_tag="PKX01_02735"
     CDS             5022..5543
                     /locus_tag="PKX01_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75067.1"
                     /translation="MSTIDAIAARHGFSADAGRAAYSALQAGGFTQAQFSHPELGGMG
                     QWMSGGMMMIGDMFNDGLKWRVGALLTELAQHREPAPAMAPMGGGFAQWSNWWPAEFG
                     SPSTSGSQNDMAYAWFPHMAKLLVKLGGDVFAFDTGNLSIYGVQQQQGGGIASVVLNT
                     SNGPMGLLGLNRG"
     gene            complement(5652..5927)
                     /gene="infA"
                     /locus_tag="PKX01_02740"
     CDS             complement(5652..5927)
                     /gene="infA"
                     /locus_tag="PKX01_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMZ75068.1"
                     /translation="MAKEELIEMQGVVSEVLPDSRFRVTLDNGHDLIAYAAGKMRKHH
                     IRIIAGDKVSVEVSPYDFSKARITFRHIEGRHAAPPPPAGFTGKRRH"
     gene            complement(6026..6232)
                     /locus_tag="PKX01_02745"
     CDS             complement(6026..6232)
                     /locus_tag="PKX01_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMZ75069.1"
                     /translation="MATGIVKWFNDSKGFGFITPDAGGDDLFAHFSAIQSKGFKSLKE
                     GERVEFDVTTGPKGQQASNIRPAN"
     gene            complement(6502..10542)
                     /locus_tag="PKX01_02750"
     CDS             complement(6502..10542)
                     /locus_tag="PKX01_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ75070.1"
                     /translation="MTRGSLRLYAAPLIAVLAIIALCTHVVLAQRRESQTSAETAARN
                     AAVVLADQLQSAFDQTDALLVSVGQRYVRARLDGPIGLARDIEPIRGEMPHHPLIKRI
                     GIADEQGIVVFNTGYTPATTDRPNVSARSYFKAAQAGEKGLMFDGPLQAKLDGVWVIA
                     LARRIDGPRGEFLGVVFAILPVESIGRLISRVELGSAGIVNLRTLDLAQVVRRPELQG
                     PGRDVGNQNVSQTIRDLLRDAPGRDRYVYTTVAPIDNTERVYVYQKLPQAPFWMTVGL
                     ATADFATSWQQTGALLALLGAVVAWTLLRATRRIVHQNRDLDRRLLEKEAAEAAARSS
                     EQRLRTILDGVDAAIYLKDPAGRYLFANAAMCRMLGLALDDVIGADDGRLFDDATAAR
                     LRADDRRVLDDGETVHGEETRTLRAGGQSATYLTTQLPLRRPDGSIEALCGISTDISE
                     RVRDRIALQSSESRFRAVVEQALVGIFISQGGIFKYANPRCVEMLGYTDVSELVDRVS
                     VLDHIDPAQRAQVADNIRRGEGGEGGALHDLDLVMLTRDGRPVDVEAYAVNTEVDGRP
                     ARLAVILDVSERARLMRELDQHRHHLEQLVQERSAELHVQAREYEDLYNGAPCGYHSL
                     GPDGVFQRINDTELGWLGYTRDELVGCRAIPEIMTPASQAVFRENFPRIAAGATIKDL
                     EIELIRKDGSVFPVLVSASPQFGPDGQMICTRSILVDYSNLRQQRQTLRRVLTASPMA
                     VRIARASDSRVLFMNKAFCDLVHQDEVAALELDVSAFYVDPGVFGDIRNALGRGETIL
                     NRLVELHLPLQPEAPHVWVLASYMPIEYDGQPAALAWLFDVTELQEARTAAEAATRAK
                     STFLANMSHEIRTPLNGVLGLAQIGFRDSAGRKAQQTFSGILDSGKLLLTVINDILDF
                     SKIEAGKLDIEDVPIDPARIVETALRSVRPQAEAKGIALEAVPDGLPSACLGDPSRIS
                     QVLLNLLTNAVKFTERGEVVLRACVREGALVFKVADTGIGIAPDVLGRLFRPFEQADG
                     SITRRFGGTGLGLVISRRLAELMGGTLDARSKPGAGSTFILRLPLRLTDRPVAPGHTP
                     ATGGRQRLKGLRLLVAEDNTVNQLVIDNILQAEGALVQIVDDGLAAVEAVARSAQPFD
                     AVLMDVQMPRMDGLEATRQICQASPGLPIIGQTAHALRSEHERCLDAGMVATVTKPID
                     IEVLVSALLEHVGGAGRIALPAPEPRPTPVPEAIIEWTALSDRYPGMGTLIDRITTLF
                     VAHHAHDGERLRQMADAVDLHALEALAHELKGVGGTLCAPEVERLAVRTLAAARAGTA
                     DAVELARALADALERLLDAVQQRRAG"
     gene            complement(10860..12116)
                     /locus_tag="PKX01_02755"
     CDS             complement(10860..12116)
                     /locus_tag="PKX01_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75071.1"
                     /translation="MSAKRHASLGIHGDHASAGVRRLHLLRRVRRAALVGAALLALAA
                     LGVAGQRYAASRALADSAARRAVTPVSLVQPKAAAGEDLLRLPGTLQGYAETPIYART
                     SGYLAAWTKDIGDSVKQGELLARIDTPEVAQQLNEARAAHDQAVAALRLAASSLERWE
                     TLRKQDSVSQQELEERRNTFAQTKAAEASTAATVQRLQTQLGYAQVTAPFAGVVTRRN
                     ADVGNLVDAGGSSRQLFVLTRTDPLRLYVQVPQGYVRQVKVGDTVPVTLQEWPGREFA
                     GTVARTARALDITTRTLQVEISLPNPRGELLPGAYAQVAFRARAGSAEGALKVPSNTL
                     LFRPEGVRVAVVGDDGAVRLQPVTIRRELAVEVELAAGSLKPSDRLVLNPPDALAEGD
                     MVQGVVPARTASADPASAGPGESRKP"
     gene            complement(12203..15364)
                     /locus_tag="PKX01_02760"
     CDS             complement(12203..15364)
                     /locus_tag="PKX01_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ75072.1"
                     /translation="MWIVRIALQRPYTFIVMALLIVLATPLVLLRIATDVLPEINIPV
                     ISVIWTYTGMSAQEMAQRVTGPNERLLATTVNDVEHFESQSYAGIVIIKLFLQPKASV
                     EAALAQIASTEGGALRQLPPGITPPLVIRYTASAIPVVQLGISSASLPEQVMFDTAVN
                     TLRPRLVSIPGVALPFPYGGKSRVVSVDLDLPALQAKGLAPLDVVNAINAQNLILPGG
                     TAKIGATEFAVKMNGSTRSVEELNDLPVRLGSGAAVYLRDVAVVRDGFSPQTNIVRQD
                     GKRGVIQSVLKNGGASTLQIVDNLRTALADALPSLPEDIRVTPLFDQSVFVRAAIGGV
                     VHEGLIAACLTAAMILLFLGNWRTTCIVAISIPLSVLASILTLYALGETINIMTLGGL
                     ALAVGILVDDATVTIENIERYLHLGHDLETAILEGAGEIAVPALVSTLCICIVFVPMF
                     FLTGVARYLFVPLAEAVIFAMLASYLLSRTLVPTLALLFMKGQHGGGAGGWLQPLYTR
                     FDAAFERLRGGYIVLLSVLLPRRRRFIAAFLAVCALSMGLYAVLGRDFFPAVDAGIVR
                     LHFRAPSGTRIEETARLADRIEAALRELIPADQITTLVDNLGLPNSGINLSYSNAGTI
                     GTLDGEIQVALAPGHGSSAALVERLRDALPPRFPGIEFFFQPADVVTQILNFGLPAAI
                     DVQISGLRMAENYRVAAQLEKAVRRIPGAVDVHIHQRFDLPTVNLAMDRNRLQQLGLS
                     AANVAQNVLIPLSGSFQTAPSFWLNPRNGVSYTITVQSPQYRIDSLDSLLQIPVAGSA
                     GTPGQLLSNLVQVGSGREPAIVSRYNIEPAVDLYVSVHGRDLGAVVADVEREVEAVRG
                     LLPKGSRISLQGQARTMNSSFIGLGIGVVMAVVLVYLLIVVNFQSWVDAFIIITALPA
                     ALAGIAWMLFLTGTPLSVPALTGAIMTVGVATANSILVVSFARQRLLAGAPPLSAALE
                     AGATRLRPVLMTALAMIMGMLPMAIGLGEGGEQNAPLGRAVIGGLLWATLSTLVFVPL
                     VFAAVHRGFARRAARRAALAGSI"
     gene            complement(15516..16004)
                     /gene="moaE"
                     /locus_tag="PKX01_02765"
     CDS             complement(15516..16004)
                     /gene="moaE"
                     /locus_tag="PKX01_02765"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HMZ75073.1"
                     /translation="MSTTGFRIQVQEADFDTGAEIAALSAGRDDVGAVASFVGLVRAD
                     KSACQTGHVSAMTLEHYPGMTEKALEAIVDEAAGRWSLQGVRVIHRFGRLLPGERIVF
                     VGVASSHRGDAFAACEFIMDYLKTRAPFWKKEDTPAGGRWVDAREADDSAAARWAQPG
                     GD"
     gene            complement(16006..16260)
                     /gene="moaD"
                     /locus_tag="PKX01_02770"
     CDS             complement(16006..16260)
                     /gene="moaD"
                     /locus_tag="PKX01_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HMZ75074.1"
                     /translation="MSVKVLYFASLRETLGYSHETLTLPTQIATLGDLRTHLARRGGV
                     WEALGEGRNVRAAINQEMAGPERPVADRDEIAFFPPVTGG"
     gene            complement(16257..17480)
                     /locus_tag="PKX01_02775"
     CDS             complement(16257..17480)
                     /locus_tag="PKX01_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HMZ75075.1"
                     /translation="MSSTLRSYEDVRDALIALARPVVETEVLPTAEALGRVLADAVVS
                     PLEVPPLDNSYMDGYAVRIADLPAPGTRLPVSQRIPAGSVGSSLQPGTAARIFTGAPI
                     PAGADAVVMQEFCSPDGDGVVIDHLPRPGEWIRRAGEDIARGQTILHAGDRLTPQRLG
                     LAASVGAAHLTVRRRLRVALFSTGDELVMPGEALPPGAIYNSNRFTLRGLLEGLGCQV
                     DDRGIVPDRLDATRAALRGAAEVSDLILTSGGVSTGEEDHIKPAVQAEGRLDSWQVAM
                     KPGKPVAFGHVNGAAGTAGAAFIGLPGNPVSSFVTFLVLVRPFILRAQGVREVLPRAL
                     PLTAAFDLPRPDRRREFLRARLGADGRVEIHPNQGPAVFTSTVWAEGLVDNPPGHVVQ
                     AGDRVHYIPYSELLS"
     gene            complement(17482..17973)
                     /gene="mobB"
                     /locus_tag="PKX01_02780"
     CDS             complement(17482..17973)
                     /gene="mobB"
                     /locus_tag="PKX01_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HMZ75076.1"
                     /translation="MKVFGFAGWSGSGKTTLMERVIPLLTGRGLKVSVIKHTHHRFDL
                     DQPGKDSWRHREAGAAEVLLISERRWALMHELRDEPEPDLAAQLARMSPCDLVLVEGF
                     KHLGLPKLEIHRPAHGRPLLHLSDPAIVAVATDGALATDLPCLNLNDPAAVAGFIVDH
                     LEL"
     gene            complement(17978..19333)
                     /locus_tag="PKX01_02785"
     CDS             complement(17978..19333)
                     /locus_tag="PKX01_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75077.1"
                     /translation="MTTRLRSADPEPADRAHSTGTAGCGGRAETLNATCQCITLDRPA
                     LREALARDPRDGALTALLAESRPHLFSDAMAFVAQAHLEHMAEVVAAIERVVAQPAWQ
                     AAVLADAPVAARRPGAAQGVFLGYDFHLGPDGPRLIEINTNAGGGLLSAVLGRAQRAC
                     CAEVGSLMRAAVPTGTDDAETAFLAMFRAEWQAERGDAPLRRIAIVDDRPGDQYLLPE
                     FLLFQRLFERHGIAAVVCDPAELRCERGCLWHDGQRVDMVYNRLTDFALDEPAHAALR
                     AAWNTDAAVITPHPRAHALYADKRNLVRLSDPDLLRGLGVDAETQAVLGAGVPRTEAV
                     TPDRAEALWAARRGLFFKPASGFGSRGAYRGDKLTRRVFAEILAGDYIAQALVPPSER
                     RVTVDGAAVDLKMDLRNYVYGGQVQLVTARLYRGQTTNFRTPGGGFAPVLAVPCDDAR
                     G"
     gene            complement(19418..21376)
                     /locus_tag="PKX01_02790"
     CDS             complement(19418..21376)
                     /locus_tag="PKX01_02790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMZ75078.1"
                     /translation="MDIDTAFAADGPLAAAIPGFAPRPQQLEMARQIADTLRANRVLV
                     AEAGTGTGKTFAYLVPALLNGGKVILSTGTKTLQDQLFNRDLPTVRGALKVPVAVALL
                     KGRANYVCHYHLARNLVDGRFQSPQDAVHLRAIGRFLEITRSGDKADCPEVPEDAPAW
                     MYATSSRDNCLGQDCPNIKECFVAAARRAAMEADVVVVNHHLFFADVMLRDEGMGELL
                     PSCNAVIFDEAHQLPETASLFFGESVSTAQVLELARDTRSETVAAARDCVDLIEATRS
                     LEKAARDLRLAVSAENARFGFAQLADNPGFHEAVAHLDDELGAFRILVESQAERSEGL
                     AACLRRTQEIQERLTRWRAPENTDWVRWVEVFSQTLALNATPLSIASIFKRQLDGHPR
                     AWIFTSATLAVGPDFGHYCRELGLHWLEPPPDTALWGSPFDYPQQAILYAPQDMPDPN
                     SPVYPEAVARVAYPLIRAAQGRTFVLCTALKAMRRIHEVIADKLAAEQLDLPLLLQGE
                     GSRSELLARFRRLGNAILVASQSFWEGVDVPGDALSVVVIDKLPFAPPDDPVLAARIE
                     HLQKSGRNPFMEYQLPRAVINVKQGAGRLIRTERDRGVLAICDPRMLSKPYGRRVWQS
                     LPPMRRSKVEAEVVAFLEDLPPPAAGGC"
     gene            complement(21381..22196)
                     /locus_tag="PKX01_02795"
     CDS             complement(21381..22196)
                     /locus_tag="PKX01_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75079.1"
                     /translation="MGRGRFTVLRGAMAAIVGLGVPAVPAQAPVGRLEIIPLRHRPAD
                     QIVAAIRPLVAPGGALSAMGDKLVLRTSPANLAELRRAIEALDTPLRRLVISVRQGDV
                     RDFAMADGGVGGIFRPGDSRVVIRGDARQGSAREQIDQQVEVIEGGQALIRVGQSVPL
                     RMRQGYATPDGGWVTADSIALVDVGSGFYAAPQLVGEQVRIEIRPEQARLDAGGQVES
                     ARLATTVSGPLGAWIPLGGSTTESTRNAAGLSGAAQGRETLSRQVWLRVDALD"
     gene            complement(22204..23316)
                     /gene="zapE"
                     /locus_tag="PKX01_02800"
     CDS             complement(22204..23316)
                     /gene="zapE"
                     /locus_tag="PKX01_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HMZ75080.1"
                     /translation="MPHRILNVSEKGMLDAYEGLLRARGYKSDPAQRAAAQRLQGMYS
                     ELVAFKAARRGAIRKLLSRPKMPRSVYFWGGVGRGKSFLMDCFFDSVPYQRKRRVHFH
                     AFMQEVQNDLKNLNHEPDPLQKVADRIARQTRLLCFDEFHVSDIADAMILGRLLDALF
                     QRGTIFVMTSNYPPDGLYPNGLQRINFLPTIDMIKRRFDVVEVDHGTDYRLRSLEKME
                     AFLVPGDDVADKRLASDFAQIAGTPGEGGEIEVLERTLKVVRQAPGVIWFDFATLCGG
                     ARSQNDYLEIAREHHTLILSRVPKMNAGMASEARRFTWLIDVLYDHRVKLLVSAECEA
                     YDLYREGHQANEFQRTVSRLMEMRSREYLAEAHRVD"
     gene            complement(23425..24846)
                     /gene="lpdA"
                     /locus_tag="PKX01_02805"
     CDS             complement(23425..24846)
                     /gene="lpdA"
                     /locus_tag="PKX01_02805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ75081.1"
                     /translation="MAKQFDVLVIGGGPGGYVAAIRAAQLGFSTACAESNPYADPKGE
                     PRLGGTCLNVGCIPSKALLHTSHLFEEAGHAFEEQGISVGKPKIDVPTMIGRKSKIVE
                     QLTQGIKGLFKKNKVTFLAGHGSFVAPIDGGWQVKVGEEVVEAKQVIVATGSTARHLP
                     GIPVDNKLVCDNIGALDIDAVPKRLGLIGSGVIGLEMGSVWNRLGAEVTILEAMDSFL
                     AAADQDVAKEALKLFTKQGLDIKLSVKVGAVTVGKKSVTVEYETKDGAQKAEFDRLIV
                     SVGRVPNTAGLNAEAVGLKIDERGFVDVDGHNRTNLPGVWAIGDVVRGPMLAHKAMEE
                     GVAVAETMAGQKGHTNFDTIPWVIYTSPEIAWVGKTEQQLKAEGVAYKVGKIPFLANG
                     RALGAGAPAGFVKMLADANTDRVLGVHIIGANASELIGEAVVTMEFAGASEDLARICH
                     AHPTLSEVVHEAALACDKRPLHF"
     gene            complement(24925..26109)
                     /gene="odhB"
                     /locus_tag="PKX01_02810"
     CDS             complement(24925..26109)
                     /gene="odhB"
                     /locus_tag="PKX01_02810"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMZ75082.1"
                     /translation="MLIEVKVPQLSESVSEATLVSWHKKEGDTVSRDENLIDIETDKV
                     VLETPAPADGVLVKIVKNGGDSVTSGEVIATIDTEAKAAAAPAAAAAPAPAAAPAAAA
                     PAAAVSAASPSARKILDEKGIAAADVAGSGRGGRVTKEDAVAAQPKAAAAAPAVAVPT
                     GDRVEQRVPMTRLRARIAERLIQSKNENAILTTFNEVNMAPIMALRKQYGDKFEKAHG
                     VRLGFMGFFVKAAVAALKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDV
                     DQMSIADIEKKIAEFGQKAKDGKISIEELTGGTFSISNGGVFGSMMSTPIINPPQSAI
                     LGIHATKDRAVVENGQVVVRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLIL
                     DV"
     gene            complement(26149..28974)
                     /locus_tag="PKX01_02815"
     CDS             complement(26149..28974)
                     /locus_tag="PKX01_02815"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMZ75083.1"
                     /translation="MMKQLFNTSQLFGSNAPFIEELYEAYLDNPDSVSDEWRSYFDTL
                     QNTPGNGRDVAHYPIINAFADMAKRGPVRTVAAGGGDQKQVSVLQLINAHRFLGTRWA
                     QLDPLKRTERPEIHELELSHYGFTEGDLNQTYNTGSFHGLPGDHATLREILDAVRQTY
                     CGSIGAEYMYITDIAEKRWIQARLEPTRGKPAFPAEQQRRILERLTAAETMERYLHTK
                     YVGQKRFSLEGGESTIVAMDELIRVAGSQGVAEIVIGMAHRGRLNVLVNTLGKSPKML
                     FSEFEGKAASDLSAGDVKYHMGFSSDVMTSGGPVHLTLAFNPSHLEIVNPVVEGSVYA
                     RQTRRGENGKAEVIPVIIHGDAAVAGQGVNQEMLNFAQTRGYGTGGTIHVVVNNQIGF
                     TTSDPRDYRSSLYCTDIFKMAEAPIFHVNGDDPEAVAFVTGLAVEYRQTFKKDVVIDI
                     VCYRKLGHNEADEPMVTQPLMYKKIAKHPGTRKLYAEKLVQLGVCKAEDPDQFIKDYR
                     AALDKGELVTNPVLSDFKRQFSQDWSPYVNKKYTDKCDSRVPMAELKRLSKALTTTPE
                     GFVLHPRVQKIIDDRKAMGEGKLALDWGMGENLAYASLLVQGYGVRLSGEDVGRGTFF
                     HRHAALHDQNRDQWHDGTFHPLKNIREGQGPFYCYDSVLSEEAVLAFEYGYASAEPNQ
                     LVIWEAQFGDFANGAQVVMDQFISSGEAKWGRQCGLVMMLPHGYEGQGPEHSSARIER
                     YMQLCAEMNMEVCIPSGPSQIFHLLRRQALRKLRKPLVIITPKSLLRHKDAVSSLDDL
                     SKGTFQTVIGEVDDLDAKKVKRVLLCSGKIYYELLAHRRANNIDDIAIVRMEQLYPFP
                     ADAFSAEVNKYPNAKEVVWVQEEPRNQGVWYWLASRHHLDTNLSDDHKFLLVARPAAA
                     SPAVGYYAKHNAQQKAVIENAFGEINA"
     gene            complement(29137..30432)
                     /gene="gltA"
                     /locus_tag="PKX01_02820"
     CDS             complement(29137..30432)
                     /gene="gltA"
                     /locus_tag="PKX01_02820"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HMZ75084.1"
                     /translation="MTTQRTAALNVDGKSVEFPIMSGTHGNDVIDIRTLGGKTGLFTY
                     DSGFLSTASCQSKITYIDGDKGELLYRGYPIEQLAEQCNFLEVTYLLKNGELPNTTQK
                     VDFENTIKRHTMVHEQLAKFYSGFRRDAHPMAVMTGVVGALSAFYHDAMDFADAEHRN
                     VSFNRLIAKMPTIVAMAYKYNMGMPFMYPDNELDYTANFMRMMFGNPCEKYVPNPTLV
                     RALDIIFTLHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEACLKM
                     LEEIGDVSRVGEYINRAKDKNDTFKLMGFGHRVYKNFDPRAKLMRKVCYDVLTELGLE
                     NDRLFKLAMELEKIALEDQYFVEKKLYPNVDFYSGIVQKALGIPTEMFTCIFALARTV
                     GWMTQWEEMITDPEYKIGRPRQLYIGAARRDVVPLAQRG"
     gene            complement(30490..30726)
                     /locus_tag="PKX01_02825"
     CDS             complement(30490..30726)
                     /locus_tag="PKX01_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HMZ75085.1"
                     /translation="MTLNRGRVRWQCRRALLELDLVFTRFLDTHFDHLTDDQVADLES
                     LLVVEDHDLWAMVNGSKPVSDDRWKEMVKLLRAG"
     gene            complement(30723..31433)
                     /locus_tag="PKX01_02830"
     CDS             complement(30723..31433)
                     /locus_tag="PKX01_02830"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HMZ75086.1"
                     /translation="MNKRTVQFKIYRYDPDKDNQPYMQDISVELEESDKKLLDALVRL
                     KAKDDSLSFRRSCREGVCGSDAMNINGKNGLACLTGIDDLAQPITIRPLPGLPVIRDV
                     IVDMTQFFKQYHSIKPYLINNEPAPERERLQTPEDREELNGLYECILCACCSTSCPSF
                     WWNPDKFVGPAGLLAAYRFLADTRDQATSERLDNLEDPYRLFRCHSIMNCVDVCPKGL
                     NPTKAIGKIKDMMLRRAV"
     gene            complement(31454..33247)
                     /gene="sdhA"
                     /locus_tag="PKX01_02835"
     CDS             complement(31454..33247)
                     /gene="sdhA"
                     /locus_tag="PKX01_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HMZ75087.1"
                     /translation="MKVAKRTFDAVIVGAGGAGLRAALQLSEAGMKTAVLTKVFPTRS
                     HTVAAQGGVAASLGNSTEDNWHWHMYDTVKGSDWLGDQDAIEFMVKKANEVVVELEHY
                     GMPFDRTDNGKIYQRPFGGHSQNYGQTPVSRSCAAADRTGHAMLHALYQRNVRANTQF
                     FVEWMALDLLRDAEGNVLGVTAMEMETGEVSIFHSRATIFATGGAGRIYYSSTNAFIN
                     TGDGVGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNASGERFMERY
                     APNLKDLASRDVVSRSMVTEINEGRGCGSDKDHVLLDITHLSPETIMKRLPGIREISI
                     QFAGVDPIKAPIPVVPTCHYQMGGIPTNYKGQVVVPQDGNPNVPVHGFYAAGECACAS
                     VHGANRLGTNSLLDLLVFGKSAGETVVADFKAGQLPLKPLPDNAADVSLARIARLEGQ
                     TGGEEVADVRIAMQRTMQKHAGVFRFADMLKEGAAKILEVAERAKVTQIKDKSQVWNT
                     ARIEALELDNLIEVAKATMVSAEARKESRGAHVRDDAIDCAETPNGRDDKNWLKHTLW
                     YSEGNRLDYKPVNLQPMSPEVEPIALAKRTY"
     gene            complement(33248..33595)
                     /gene="sdhD"
                     /locus_tag="PKX01_02840"
     CDS             complement(33248..33595)
                     /gene="sdhD"
                     /locus_tag="PKX01_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HMZ75088.1"
                     /translation="MVKPIIVGAHYGFRDWLAQRITAVVMAVYSILFAVSALATLDGT
                     YESWRGLFSGGFMKFATFLFFMSLFYHAWIGVRDIFMDYIKPTGLRLTLHSVVALLLI
                     GYAGWAAQILWRL"
     gene            complement(33589..33978)
                     /gene="sdhC"
                     /locus_tag="PKX01_02845"
     CDS             complement(33589..33978)
                     /gene="sdhC"
                     /locus_tag="PKX01_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HMZ75089.1"
                     /translation="MTEVTLKKQRPKHLALNEIRLPLPGKVSILHRVSGAGLFLMLPV
                     LLALFGASVGSPESYAAYKDVVSNVFVKLLLVGLLWAFLHHFCAGIRFLLLDVHVGVD
                     KESAVSSARTVLIVSLALTVILVAKLW"
     gene            complement(34094..34822)
                     /locus_tag="PKX01_02850"
     CDS             complement(34094..34822)
                     /locus_tag="PKX01_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMZ75090.1"
                     /translation="MRQDSPTFSPLYRQIKNLMLNALESAEWRPGQAIPSEQELALRF
                     SVSQGTVRKAIDEMAAENLLVRKQGKGTYVASHNDPRALFRFLRLVPIDGELAHPQSI
                     PLDCWRAKAGNEAARMLGIELGAPVTILRRLLKFGGRPVVVDEIYLPGEVFHGLTMEV
                     LQGSTGSLYGLFEARFGIRMIRAEERIRAVAADRMVGETLGVAEGTPLLSVERVTYTY
                     GDRPVEWRRGLYSTADHYYLNELS"
     gene            35053..36045
                     /locus_tag="PKX01_02855"
     CDS             35053..36045
                     /locus_tag="PKX01_02855"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMZ75091.1"
                     /translation="MAKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKAVKGVIMELEDCAFPLLAGVIATDDPNVAFKDAQVALLVGARPRGPGMERKDL
                     LTENAKIFTVQGAAIGQYADPDVKVLVVGNPCNTNAYIAKEIAVKYGRVQAKNFTGML
                     RLDHNRALSQLATKTGRDVSSLKNLVVWGNHSPTMYADYRFATSNGDKVPALVNDEAW
                     NRDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSY
                     GIPEGIIYGFPVTTVNGEYTIVQGLEIDEFSRSKMTNTLNELLEERDGVKDLLG"
     gene            36143..37132
                     /locus_tag="PKX01_02860"
     CDS             36143..37132
                     /locus_tag="PKX01_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HMZ75092.1"
                     /translation="MTADHHPDSVLFQGEKPFPVLPAVDHYAGSEKLMRKALALQQEI
                     GPLFDITCDCEDGAHAGAEAEHARMAASIVMSGDNRFGRVGARIHDITHPHWEQDMDI
                     LVGEAGARLAFLTLPKPRSVADAARQIDALRSAEERHGIEQAIPVHVLIETHGALRDA
                     WEIAALDRVESLDFGLMDFVSGHHGAIPGAAMRSPGQFEHPLIIRAKCDIAAAALAHG
                     VVPSHNVTTELKDLAYIRRDAERARREFGYLRMWSIHPNQILPIVEAMRPDFSEVEEA
                     TAILAGAQDAGWGPIQHAGKLHDRASYRYYWELLKRARATGMNLPDDALARFF"
     gene            complement(37220..38464)
                     /locus_tag="PKX01_02865"
     CDS             complement(37220..38464)
                     /locus_tag="PKX01_02865"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HMZ75093.1"
                     /translation="MKRVDDFRLRFGRQEYVPIMIGGMGVDISTADLALEAARLGGVG
                     HISDAMVKTVADRRFKTKYVKEKLQQYKYNVANPDKSEVQFDLGHLAEATHLHVGRTM
                     EAKRGQGLIFVNCMEKLTMNAPKETLKVRLTAAMDAGIDGITLAAGLHLGSFALIEDH
                     PRFRDVKLGIIVSSLRALQLFLRKSARTQRLPDYVVVEGPLAGGHLGFGMDWAQYDLA
                     TIVAEIRDWLKVEQLDIPLIPAGGIFTGSDAAAFLEAGAGAVQVATRFTVSQECGLPD
                     AVKQQYFRADEDDIEVNQISPTGYPMRMLKNSPAIGSGIRPNCEAYGYLLDAKGSCAY
                     IEAYNRELALHPAEKKLSVLDKTCLCTHMRNFDCWTCGHFTYRLKDTTRLDENGQYRL
                     LTAEHIFRDYQFSTDQKIALPD"
     gene            38718..39611
                     /locus_tag="PKX01_02870"
     CDS             38718..39611
                     /locus_tag="PKX01_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ75094.1"
                     /translation="MPEAVPPSSEYAVLFADLVGSTELYERVGDKAAFEQVDRCIRGM
                     RIAVETKQGRVVKHTGDGLMAVFRDADSAADATLLIHQTVKELPAAGDQKLALRIGFH
                     FGPVVVAGTDVFGETVNFAARLAELASPGKAITSSETARRLGPEWRSVLHTLPPRAIR
                     GLSRPVEVCELMCEAVGELTIVQSDHFLLATEPELRLYLDSKTLLLNATRPSARIGRD
                     TAADLVVADTQSSRRHAEIELRGDKFVLIDRSSNGTFVQIDGEKEFLLSREEVVLRGR
                     GHFSLGRSCANSPQVVDFVCL"
     gene            complement(39634..40587)
                     /locus_tag="PKX01_02875"
     CDS             complement(39634..40587)
                     /locus_tag="PKX01_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ75095.1"
                     /translation="MALDPQARALLDMVYRVGAPRFHELDVHQARHSFEKLLFALRPE
                     APPVASVTEVPIPRAHARDGVIMARLYRPLNSHADEALPITFYFHGGGWCVGNVASYD
                     VLGRQLANLSGGAVLSVDYALAPEHPFPGAVVDAQRAVDWALANAVLLGVDPARFALA
                     GDSAGGTLSAVLAHQMRNARVHAARLQLLIYPCVEILSQRPSRKTYSNGYFLDNESMA
                     WFFDKYLPNAEDWSDWRASPLLAPRFDDLPPAAIITAGCDPLTDDCLAYADALRAAGV
                     PVSVHGFDGMVHGFFTLGKFFPQANEAVTRAGEALRDALKA"
     gene            complement(40835..40919)
                     /locus_tag="PKX01_02880"
     tRNA            complement(40835..40919)
                     /locus_tag="PKX01_02880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40883..40885),aa:Leu,seq:caa)
     gene            40963..41991
                     /gene="queA"
                     /locus_tag="PKX01_02885"
     CDS             40963..41991
                     /gene="queA"
                     /locus_tag="PKX01_02885"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMZ75096.1"
                     /translation="MALTLDDFDYPLPPELIAQAPLPRRTDSRLLHVAEPLADLHFAD
                     LPALLRPDDLLVFNDTRVLHARLFGVKDSGGRVEVMIERPLDEHEALAQIRASKSPKA
                     GTRLRLEDALDVDVLGRAGDFFHLRFPPDAPLVTLLERHGRLPLPPYIERAAGEADES
                     RYQTVYARHPGSVAAPTAGLHFDDALLAQLAAQGVRSATVTLNVGAGTYQPVRVQDLT
                     QHRMHTEAYHVPDATVAAIDTARATGGRVICVGTTSLRALESAARDGSLRAGQAESDL
                     FILPGYRFRVADALITNFHLPKSTLLMLVSAFAGMDTLRSAYAHAVAQRYRFFSYGDA
                     MFLTRKPA"
     gene            41988..43100
                     /gene="tgt"
                     /locus_tag="PKX01_02890"
     CDS             41988..43100
                     /gene="tgt"
                     /locus_tag="PKX01_02890"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ75097.1"
                     /translation="MKYELLATDGAARRGRLTLNHGTVDTPAFMPVGTYGSVKGISPQ
                     DLRDIGAQICLGNTFHLWLRPGLDVMTAFGGLHQFMAWDGPLLTDSGGFQVFSLGALR
                     KISEEGVRFASPVNGDKLFLTPEESMRIQRVLNSDVAMIFDECTPYPASETEAGDSMR
                     LSLRWARRSRDEFDRLENPNALFGIVQGGMYESLRDESLAGLTEIGFHGYAIGGLSVG
                     EPKEDMQRILHHTAPRLPKDSPRYLMGVGTPEDIVFAVQAGIDMFDCVMPTRNARNGW
                     LFTRHGDIKIRNARHKADTRPLDDSCDCYTCRHFSRGYLHHLNRLNEILGARLATIHN
                     LHYYQQLMRELRDAIAHGRLTDYVARFHRERAGQDA"
     gene            43172..44104
                     /locus_tag="PKX01_02895"
     CDS             43172..44104
                     /locus_tag="PKX01_02895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMZ75098.1"
                     /translation="MQVLRGIPDHAEHGCVLTIGNFDGVHRGHQALLARLAAKAQETG
                     LPAVVLTFEPHPREYFTHEVRPVRLTSLREKVGLLEQHGVDRIVIGRFNERFAGLSAE
                     AFIEDVLIHGLGVRHLLIGDDFRFGRGRKGDFALLRATGERAGFTVEAMPTLADADGR
                     VSSSAVREALAAGDMPRAARLLGRPYSISGRVLHGDKIGRTIGFPTANIQLKHRSPPL
                     MGIFTVSVDGVAERPWPGVASIGVRPTINDAGRPSLEVHLFDWHGDCYDAHLRVNFLA
                     WQRAEERYDSLDALTAQIARDADQARAHFARNPL"
BASE COUNT         7385 a        14368 c        15192 g         7329 t
ORIGIN      
        1 gggcggggtg cgtcgtcggg gccgggggcg gggtcggcgg aggcgtcgtc tagggggccg
       61 aagacggcgg cgttgagggc gccgagcagg gccgccaggc gggcgtcgcc gcccaggaca
      121 cgacgggctt cggcgaggcc gcccatctgg gcgaagacgt ggaagtaggc gaagtgctcg
      181 ggggtgatct catcgagggt gtcggcgttg gcgcggatct ggttgccgag gaggtggagc
      241 cagcgctcgg cctcggcgga aaggtcggca ttgtgcagca tccaggcttc gaagcgggtg
      301 ccgagttggg cggcacggcg ttcgctggcg gcgggaaaga cgaggttgcc gtcggcgtcg
      361 agggtgatcc attcgcggct ggcggggtcg atggtgtcgt cgatggggag gccgagcagg
      421 cggcgcggac tgtagcggcg ccgggcgggg gtctcgcgga cgaggccgcc ttcgccgtag
      481 gtgtcggcat cgccgtggaa gtcgacgacg ccgacgaagt cgaagagggt gaagacgggt
      541 ttgttggggg cccggcgggt gccccggccg cgcatctgct ggtagaggat ggtggagcgg
      601 gtgaagcggg cgaagacgag gttggcgacg tcggcgcagt cgaagccggt gtcgagcatg
      661 ttgacggaga cgaggatgcg ggggaattcc tggcgcttga agcgctggat cttggtgagg
      721 ccgtcggtcg tgtcgtcgtc gccgcagccg gacacgacgt agtcggcgta ggccacggcg
      781 gggtcgggct tgcagtcggc gaaggcgtcg tcgaagagct tggcgagggt ggcggcgtgg
      841 cgcttggtga cggcgaagac gatggtcttg ccaagcagag gcttgcgcag gatgcctttg
      901 tggtcacgga agccttcgtc catgaccttg cggaattcgg tgacgatggc ctggttgcgg
      961 gcggggatgg tgaagcgccg ttcgagggcg ttggggtcga cggtgagggt gtcgtcggga
     1021 ccgaaaagct gggtaagctc ggtgcgcgtg tcggcgtcca gggcgttcca gtccagttcg
     1081 ccgcgggtga cggtgaagcc gccttcggtg gcggtgcgca cggtgcgggc acgatagatc
     1141 tggtagggca ccaggtggcc gtcggcgatg gcctggcgca ggccgtagga gaaggtgggc
     1201 tgctcgactt cgaagaagcg cagggtgtcg cgcacgaaga ggcggtcttc gtcgtccggg
     1261 tcgggcgcgc gctcgtcgcc cggcggggcg acgcaggggg tggcggtgag gccgatctgg
     1321 aggccgtcga agtggcgcag caccccgctc cacttgccgt agatgctgcg gtggcattcg
     1381 tcggtgacga cgaggtcgaa gtagccggag gagtagtccc tgtagaggtt aaccatgctc
     1441 tgcagggtgg tgacggtgat gcgcttttcg tcctggaagc ggcgcccggc gcgcaggacg
     1501 taggcgggga aatccggcag gtgttcggcg aaggcgtctt cggcctgggt ggcgaggggg
     1561 atgcggtcca ccaggaagag cacgcgggtg atggcgtggg cttcgaagag gcgtttgatg
     1621 agggcggcgg cagtgcgggt cttgccggtg ccggtggcca tctccaccag cagcttgcgg
     1681 cggccgcggg cgatttcggc gctgagggtg tcgatgcagg cgagctggta gtcgcggccg
     1741 gcgatgcggg tgtcgatggg gacgcggtcg agggggatgc ggatctggcg gacggcggcc
     1801 cggcgttcca ggtcgtcctg cccgaagaag gtcttgaccg gccgggggtg ggcttcgcgc
     1861 tgccagtccc agaacaggat ttcgtggccg ttggccagga agacgtaggc gacgcccagt
     1921 tggcgggcgt agcgaccggc ctggtcgcgg gcggtggccg ggtcgaccgt gaagcgtttg
     1981 gcctcggtga ccgccagggc gcgcccgtgg cggtcgttca gcacatagtc ggcgatgccg
     2041 tcgtcgaggg cgacttcgta acagacgctg ttggggttgt gcagatccca gccctggtcg
     2101 gcaagctggg cgttgatggc gacacgggcg tgggcttcgt tgtcgtgcat ggccggcgat
     2161 tccattggag tgccggcatc ttaccctgcc gcgggtcggc gacggcgggc gagcgcccac
     2221 cggccccgtc gaccgcctca gtcgaacacg taagacaccg aactgagggg ctgcgccgtg
     2281 tgctggaggc attccagcac ctcgaaacgc tcggcggcgt cggcttcggt gaggggatag
     2341 ggggtgatga aggccgggcg ggacgcctcg ggctggtcgg gggacgcttt ggctcggacg
     2401 cagaagcgat agagcacggg gtcttccatg gcagatcctt ctgtgggcgg ttggggtgtg
     2461 cggagtatac gccgcagccg gggtgtgcga gtgccggggc gcaccgcagc gcggacggga
     2521 tgccgcgcgg tgccgatggg actacactgc caccgccatg cttcattacg ccgtcaaggt
     2581 ggccgtgtct gccggcctca tcgtcgcaat ctccgagatc gccaggcgct cgtcgggggt
     2641 cgctgccctg ctggcggcgc tgcccctcac gtcggtgctg gcctttgtgt ggctgtatgt
     2701 ggaaggccag ccgacggagg cgatcgcgtc gctgtcgggg cagatcttct ggctggtgct
     2761 gccgtccctg gtgctgttcg tggccttgcc ctggctgctg cgccaggggg tcggcttctg
     2821 ggcggcgctg gcgctggcga tggggctgac cgccggggcc tatgcggtgt tgcggccgct
     2881 gttgcggcac ctgggggtgg atctgtaagg gcggcggcag gcgtttatgc cggcgcgggg
     2941 ccggcggtgc ggccggcccc gtgtggggga tcgcccgtgc gggacgggcg cccggccggt
     3001 gggtgaggcg ctgcgcgccg ccacggcatt tgcctcgcgc cccggtcggg cgcatagtgg
     3061 agccgcttga ctccgcgccc ggcgccctga aaagcattcg ccccccgcat cgatggctgt
     3121 ttcctgcggg ccctcctccg cttttcctag gccctccccc atggctcacc ccgaccacct
     3181 gctctggcga cagggctgga acgcgaaccg gattcccttc catctcaccc acatccaccc
     3241 gctgctgcgg cgcttctggg acacgctgga cccggccgcg acggcgcggg tcttcgtgcc
     3301 cctgtgcggc aagagcctgg atctgatgtg gctgcacggc ctgggccacg atgtggtggg
     3361 catcgagctg agcgcggtgg cggtggggga tttcttcaag gcggcgcgcc tgcgcccgca
     3421 gcgcacccgt caggcggacc tgacctgctg gacgcaggag cggctggcga tcttctgcgg
     3481 ggacttcttc gatctgacgg aggaggatct gcagggggtg tcggcggtgt atgaccgggc
     3541 ggcgctgacc gccctgccgg aggggctgcg cgcggattac gtggcccacc tgcacgcgat
     3601 cctgccggcg ggctgccagg tgctgctgat gacggtggag gatctggacg acgatgaggc
     3661 ggcggtggag cacatggcgt cgtcggacga ggtgacggcg ctgtacgcgg ggctgttcag
     3721 cgtggagttg ctccacgcgg aataccacga ggcggtgcaa ggccgcggcg acggcgtggt
     3781 ggacgggcgc tgcatccaca aggtgtaccg gatgcgccgc gaggcccgca gcccctcggg
     3841 cacgggcggt accgtcgcgt aggcggcccc gcagggggtg tgcgcacccg ccggacagcc
     3901 ccgccgggcg gtgattccgg aaaaaacgca gcatgatgcg gtgccgcagg gtaggatgag
     3961 cgccttgtcg cacgttctgg atcgcttggt ccggcacgtc gcgacggtca ttcagcctcg
     4021 cccggtggcc cgcaatcctg cgtcaaggcg tgtttccggt ctttttcatg atccgcttgc
     4081 ggctttctgt cgaattgagt tacgacgtgg cgcctcccgg ctgcgacgtc atcttcaacg
     4141 tccacgcggc ccatacgtcc catcagcggg tggccaccga gatgatggag atcagccagt
     4201 tcctcctgcc ccaggtccac accgatgtcg ccaccggcaa ccgctacatg cgcgtgaagg
     4261 ccgaggcggg cggcctgcgg ctgcgctata gcgccaccgt ggaagtgcgc caccaccggg
     4321 cagtgcccga cggcatccac gaagtgccgg tgtcgcaact gccgacaggt gcattgatct
     4381 acctgtaccc gagccgctac tgccagtccg accgcctgta cgacatggcg cgccagacct
     4441 ttggccatct gctgccggga tacgcccggg ttcaggcaat ctgcgattgg gtgcgggctc
     4501 acgtgaagtt ccagtcggcc acctccacgg cgagcacctc ggcgctggat accctgcggg
     4561 acaaggtggg ggtgtgccgg gatttcgctc atctgatgat cgccctgtgc cgcgccctca
     4621 acattccggc gcgctttgcc acgggcatcg actacggcgc cgacgcgacg ctggggcccc
     4681 acgacttcca cgcctacgtg gaggtcttcc tgggcgaccg ctggtatctg ttcgaccctt
     4741 cgggcacggc gatcccgatg ggactgatgc gcttcggcac cggacgcgac gcggccgacg
     4801 tatccttcgc aacccttttc ggcacggtca acgctgcacc gccccgaatc gacatcaacg
     4861 cgatcgaaga cgccgccacg ggtgccctgc tgccctggcg ctgcccggac gccctgtcca
     4921 ccgacgacgg catcgggatg ctggtaggca cgcgcctcgt ctccgccgtc tgagggcgga
     4981 gcgcatccca tttacaccca tttcaatcag gagtacttcc catgagcacc atcgacgcca
     5041 ttgcggcccg ccacggtttc agcgccgacg ccggtcgcgc tgcctattca gccctgcagg
     5101 ccggcggctt cacccaggcc cagttcagcc acccggagct gggcggcatg ggtcagtgga
     5161 tgagcggcgg catgatgatg atcggcgaca tgttcaacga cggcctgaag tggcgcgtcg
     5221 gggccctgct gaccgagctg gcccagcacc gggagccggc accggcgatg gcgccgatgg
     5281 gcggcggttt tgcccagtgg tccaactggt ggccggccga gttcggctcg ccgtccacct
     5341 cgggcagcca gaatgacatg gcctatgcgt ggtttcccca catggccaag ctgctggtca
     5401 agctgggcgg cgatgtcttc gccttcgata ccggcaatct gtcgatctat ggcgtgcagc
     5461 agcaacaggg cggcgggatc gcgtcggtgg tgctgaacac ctccaacggc cccatgggtc
     5521 tgctgggctt gaatcgcggg tgaagaccgg ggcgctgcct gtgcggcggc gcagcccgag
     5581 ccccggcggg cgccgggcgt ccgacacgga ccctgacgcg gagcgccggg tacatgtcgc
     5641 gggcgtcgcg ctcagtggcg gcgcttgccg gtgaagccgg cgggcggcgg cggcgcggcg
     5701 tggcggcctt cgatgtggcg gaaggtgatg cgcgccttgc tgaagtcgta gggggagact
     5761 tcgacggaga ccttgtcgcc ggcgatgatg cggatgtggt gcttgcgcat cttgcccgcc
     5821 gcgtaggcga tgaggtcgtg accgttgtcc agcgtcacgc ggaatcggga gtcgggcagg
     5881 acttccgaga cgacgccctg catttcgatg agttcttcct tggccatggg ggtccttgca
     5941 gtccgttaac gaaaaagccc ggctgggccg ggcctttcaa tccgcctcgg gatggggaca
     6001 gatgccacac cccgatggac gacactcagt tcgccggacg gatgttggag gcctgctggc
     6061 ccttgggacc ggtggtcaca tcgaactcca cgcgctcacc ttccttcagg ctcttgaagc
     6121 ccttggactg gatggcggag aagtgggcga agagatcatc gccgcccgcg tccggggtga
     6181 tgaaaccgaa gcccttggag tcgttgaacc acttaacaat gccggttgcc atgtcttgaa
     6241 tatcctgaaa taaataacgc aaaggggatt tgcccctgaa aaatgggacg acactcaaga
     6301 cagcgggtac ttattgggag gaaccgcagg actgcggaat acgacctgaa agctacggca
     6361 ctacttggga atcgcccgcg cactatgggg gacaacgggg caaatagcaa caagaatctt
     6421 gcatcaaggg ctgtttgatt gcgcgcgggc cccggatcgc ccaatgacac cgccccgatt
     6481 gccccgacga gggccgccac cctacccggc ccgccgttgc tgaacggcgt ccagcaggcg
     6541 ctcgagggca tcggccaggg cgcgtgccaa ctccacggcg tcggccgtac ccgcgcgggc
     6601 agcggccagg gtgcgcacgg ccagacgctc cacttcgggc gcgcagaggg tgccgccgac
     6661 gcccttcagc tcgtgggcca gcgcttccag ggcatgcaga tcgaccgcgt cggccatttg
     6721 acgcaggcgc tcgccgtcgt gggcgtggtg cgcgacgaac agggtggtga tgcggtcgat
     6781 cagggtgccc atgcccgggt agcggtctga cagggcagtc cactcaatga tggcttcagg
     6841 cactggcgtg ggacgcggct caggcgcggg cagcgcgatg cgccccgcac caccgacgtg
     6901 ctccaggagc gccgagacca gcacttcgat gtcgatgggt ttggtgacgg tggccaccat
     6961 gccggcgtcg aggcagcgct cgtgctcgct gcgcagggca tgggccgtct ggccgatgat
     7021 cggcaggccg gggctggcct ggcagatctg gcgggtcgct tcgaggccgt ccatgcgggg
     7081 catctgcacg tccatcagca ccgcgtcgaa gggctgggcg ctgcgggcga cggcttccac
     7141 ggcggcgagg ccgtcgtcca cgatctggac cagagccccc tcggcctgca ggatgttgtc
     7201 gatgacgagc tgattgacgg tgttgtcttc ggcgacgagc agacgcaggc ccttgaggcg
     7261 ctgccggcct ccggtggccg gcgtgtggcc gggcgctacc ggccggtcgg tcaggcggag
     7321 gggcaggcgc aggatgaagg tgctgccggc gccgggcttg ctgcgggcgt cgagggtgcc
     7381 gcccatcagc tcggccaggc gccggctgat gacgaggccc aggccggtgc cgccaaagcg
     7441 gcgggtgatg ctgccatcgg cctgttcgaa gggacggaag agccgtccga ggacatccgg
     7501 cgcaatgccg atgccggtat cggccacctt gaagacaagc gctccttccc ggacacaggc
     7561 ccgcaggacg acctcgcccc gctcggtgaa tttgacggca ttggtgagca gattgagcag
     7621 gacctggctg atccggctag gatcccccag acaggcggag gggagcccgt cgggcacggc
     7681 ctcgagggcg atgcccttgg cctcggcctg gggccggacg ctgcgcaggg cggtctcgac
     7741 gatgcgcgcc gggtcgatgg gcacatcttc gatgtcgagc ttgccggctt cgatcttgga
     7801 gaagtcgaga atgtcgttga tgacggtgag cagcagcttg cccgaatcga ggatgccgct
     7861 gaaggtctgc tgggccttgc gcccggcgct gtcccggaag ccgatctggg cgaggcccag
     7921 caccccgttg agcggcgtgc ggatctcgtg gctcatgttg gcgaggaagg tgctcttggc
     7981 gcgggtggcg gcttcggcgg cagtgcgggc ctcctgcaat tccgtgacgt cgaagagcca
     8041 ggccagcgcg gcgggctggc cgtcgtactc gatgggcatg taggaagcga gcacccagac
     8101 atggggcgct tccggctgca ggggcaggtg cagctccacc aggcggttga ggatggtctc
     8161 gccccgcccc agggcgttgc ggatgtcacc gaagacgccc ggatccacgt agaaggcgct
     8221 gacgtccagt tcgagggccg ccacctcgtc ctggtgcacc agatcgcaga aggccttgtt
     8281 catgaagagc acgcggctgt cggacgcgcg ggcgatgcgc acggccatcg gcgaggcggt
     8341 gagcacccgg cgcagggtct ggcgctgctg acgcaggttg gagtaatcca ccagaatgga
     8401 gcgggtacag atcatctgcc cgtcgggccc gaactggggc gacgcgctca ccagcaccgg
     8461 gaagacgctg ccgtccttgc ggatgagttc gatctccagg tccttgatgg tggcaccggc
     8521 cgcgatgcgc ggaaagttct cgcggaacac ggcctgcgag gcgggggtca tgatctccgg
     8581 gatggcgcgg cagcccacca gttcgtcccg cgtgtacccg agccagccca gctcggtgtc
     8641 gttgatccgc tggaagaccc cgtcgggacc cagggagtga tagccgcagg gcgcgccgtt
     8701 gtagagatct tcgtactccc gggcctggac atgcagttcg gcgctgcgct cctggacaag
     8761 ctgttccagg tggtgccggt gctgatcgag ctcgcgcatc aggcgcgccc gttcgctgac
     8821 atcgaggatc acggccaggc gcgcgggccg gccatccacc tcggtattga cggcataggc
     8881 ctccacgtcc accgggcggc cgtcacgcgt cagcatcacc aggtccaggt cgtgcagggc
     8941 gcccccctcg ccgccttcgc cgcgccggat gttgtccgcc acctgggcac gctgggccgg
     9001 atcaatatgg tccagcacgc tgacccggtc cacgagttcg ctcacgtcgg tgtagccaag
     9061 catttcgacg cagcgggggt tggcgtattt gaagatgccc ccctgactga tgaagatgcc
     9121 cacgagggcc tgttccacga cggcccggaa gcggctctcg gaggattgga gcgcaatccg
     9181 gtcccggacc cgctctgaga tgtcggtgga aatgccgcac agggcttcga tgctgccgtc
     9241 ggggcggcgc aggggcagct gggtggtgag gtaggtggcg gactggccgc cggcccgcag
     9301 ggtccgggtc tcttcgccgt ggacggtctc gccgtcgtcg agcacgcgcc ggtcgtcggc
     9361 ccgcaaccgg gctgcggtgg cgtcgtcgaa gaggcgtccg tcatcggcac cgatcacgtc
     9421 gtcgagggcc aggcccagca tccggcacat ggcggcgttg gcaaagaggt agcgcccggc
     9481 ggggtccttc aggtaaatgg cggcgtccac gccatcgagg atggtgcgca ggcgctgctc
     9541 gctgctgcgg gccgcggcct cggcggcctc cttttccagc agacgccggt ccaggtcgcg
     9601 gttctggtgc acgatgcggc gcgtggcgcg cagcagggtc cacgccacga ccgcgcccag
     9661 gagggccagc agcgcgccgg tctgctgcca gctggtcgca aagtcggccg tcgccaggcc
     9721 aaccgtcatc cagaacggcg cctgcggcag cttctgatag acatagaccc gctcggtgtt
     9781 gtcgatgggg gcgaccgtcg tatagacgta ccggtcgcga ccgggcgcgt cccgcagcag
     9841 gtcgcggatg gtctgggaaa cgttctggtt gcccacgtcc cggcccggcc cctggagttc
     9901 ggggcgtcgt accacctggg ccagatcgag ggtgcgcagg ttgacgatgc cggcactgcc
     9961 cagctccacg cgggagatga gccggccgat ggactccacc ggaaggatcg caaagaccac
    10021 gccgaggaac tcgccccggg gaccgtcgat gcgacgggcc agggcaatga cccatacgcc
    10081 atcgagcttg gcctggagcg gcccgtcgaa catcaagccc ttttcgccag cctgcgccgc
    10141 cttgaaatag ctgcgggcgg aaacgttcgg acggtcggtc gtggccgggg tatagccggt
    10201 gttgaacacc acgatcccct gctcgtcggc aatgccgatg cgcttgatga ggggatggtg
    10261 gggcatttcg ccgcggatgg gctcgatgtc ccgggcgagc ccgatcggcc cgtcaagccg
    10321 cgcacggaca tagcgctgac cgacggagac gagcagcgca tcggtctggt cgaaggcact
    10381 ctgcagctga tcggccagca ccactgccgc attgcgcgcg gcggtctcgg cgctggtctg
    10441 gctttcgcgg cgctgggcca gcaccacgtg ggtgcagagg gcaatgattg cgaggacggc
    10501 gatcaggggc gccgcataga gccgcaggct cccccgggtc atcgatggcg tgcggtgtgt
    10561 ggtccggtct ggtatctgca tgctctggct cagtccggct ccgccgccgg cgcttcgtgt
    10621 cggacgggac gcggacgtcc cgcggacagc ctgccttccc cgccgcatgc tccacggcgg
    10681 gcgatccgct ggaacacatc gctcacccgc ccccgcgggg gtcaggactt ctgtccgaag
    10741 ggcttatcgg caccagatga ccactcttca gccggatttg acgaaaacgt caaacagcgc
    10801 acgaccgcct gcagactccg gacatcgatg cgcgccccgc cggctgaggc gcggtgcgat
    10861 cagggcttgc gagactcacc cgggcccgcg gaagcgggat cggcggacgc tgtgcgcgcc
    10921 gggacgaccc cctgcaccat gtcgccctcc gccagcgcgt ccggcggatt gaggacgagg
    10981 cggtcgctcg gtttgagact gccggccgcc agttccacct ccacggccag ctcgcggcgg
    11041 atggtgacgg gctggaggcg caccgcgccg tcgtcgccca ccaccgcgac gcgcacccct
    11101 tccgggcgaa acagcagggt gttgctgggc accttgaggg cgccttcggc gctgcccgca
    11161 cgcgcccgaa acgccacctg ggcgtaagca ccggggagga gttcgccccg gggattgggc
    11221 aggctgatct ccacctgcag ggtgcgggtg gtgatatcga gggccctggc ggtgcgcgcg
    11281 accgtgccgg cgaactcccg gccgggccat tcctgcagcg tcaccggcac ggtgtccccc
    11341 accttcacct ggcgcacgta gccctggggt acctgcacgt acaggcgcag gggatcggtg
    11401 cgggtgagga caaagagctg ccggctgctg ccgcctgcat ccaccagatt gcccacatcc
    11461 gcgttacggc gggtgaccac gccggcgaac ggggcggtca cctgggcgta gccgagctgg
    11521 gtctgcaggc gctgcacggt ggccgcggtg gaggcctccg ccgccttggt ctgggcgaag
    11581 gtgttgcggc gttcttcgag ttcctgctgg gacacggaat cctgcttgcg cagggtctcc
    11641 cagcgttcga gggagctggc ggcgagcctg agggcggcga cggcctggtc gtgggcggcg
    11701 cgggcctcgt tgagctgctg ggccacctcc ggcgtgtcga tgcgggcgag gagttcgccc
    11761 tgcttcacgc tgtcgccgat gtccttggtc cacgccgcca ggtagccgct ggtgcgggcg
    11821 tagatggggg tctcggcgta gccctgcagg gtgccaggca ggcgcagcag atcctcaccg
    11881 gcggcggcct tgggctgcac gaggctgacc ggcgtcacgg cgcggcgcgc cgcgctgtcg
    11941 gccagggcgc gggacgcagc gtagcgctgg ccggcaacgc cgagcgccgc gagggccagc
    12001 agggcggcac cgaccagggc cgcacgccgg acacggcgca gcaggtgcag acgccggacg
    12061 cctgcggacg cgtggtcacc atgaatgccg agactggcgt ggcgcttggc ggacatgtca
    12121 gacctcctgc cgggccgtcc cacggacgac cggcgcccct ccagggggcc aggaacaaag
    12181 tgagcgtgag gggcgaatca tcctagatgc ttccggccag cgccgcccga cgcgccgcgc
    12241 gacgggcaaa gccccgatgc acggcggcga acacgagggg cacgaacacc agcgtggaca
    12301 gggtcgccca cagcaggccg ccgatcacgg cgcggcccag cggggcgttc tgctcgcccc
    12361 cttcgccgag gccgatggcc atgggcagca tgcccatgat catggcaagg gccgtcatca
    12421 acaccggacg caggcgggtg gcgcccgcct ccagcgccgc ggacaggggc ggcgcgccgg
    12481 ccagcagacg ctggcgggcg aaagacacca ccagaatgct gttggccgtg gcgacaccga
    12541 cggtcatgat cgcgccggtg agggctggca cactgagggg cgtgccggtg aggaagagca
    12601 tccaggcgat gcccgccagg gcggccggca gcgcggtgat gatgatgaag gcgtccaccc
    12661 aggactggaa attcacgacg atgagcaggt agaccagcac gacggccatc accacaccga
    12721 tgcccaggcc gatgaaggag ctgttcatgg tgcgggcctg gccctgcagg ctgatgcgcg
    12781 agcctttggg cagcagcccg cggacggcct ccacctcgcg ctccacgtcg gccaccacgg
    12841 cccccaggtc gcggccgtgc acgctgacgt agagatccac cgccggttcg atgttgtagc
    12901 gggagacgat ggccggctcg cggccggaac ccacctgcac gaggttggac aggagctggc
    12961 cgggcgtgcc cgcgctgccg gccacgggga tctggagcag gctgtcgagg gagtcgatgc
    13021 ggtactgggg cgactgcacg gtgatggtgt aggacacacc gttccgggga ttgagccaga
    13081 agctcggggc ggtctggaag ctgccggaga gagggatcag gacgttctgg gccacgttgg
    13141 cggcggacag gccgagttgc tgcaggcggt tgcggtccat ggccaggttg accgtgggca
    13201 ggtcgaagcg ctggtggata tggacgtcga ccgcgccggg aatgcgccgg acggcttttt
    13261 cgagctgggc ggcgacgcgg tagttctcgg ccatgcgcag gccgctgatc tgcacgtcga
    13321 tcgcggccgg caggccgaag ttgaggatct gggtgaccac gtcggcaggc tggaagaaga
    13381 actcgatgcc cggaaagcgc gggggcagcg cgtcccgcag gcgctcgacg agggcggcgg
    13441 acgacccatg cccgggcgcc agggcgacct ggatctcgcc gtccagggtg ccgatggtgc
    13501 cggcattgct gtaggagagg ttgatgccgc tgttgggcag gcccaggttg tcgaccaggg
    13561 tggtgatctg gtcggccggg atgagttcgc gcagggccgc ctcgatgcgg tccgccaggc
    13621 gcgccgtctc ctcgatgcgg gtgccgctgg gggcgcggaa gtggaggcgg acgatgcccg
    13681 catccaccgc ggggaagaag tcgcggccga gcacggcgta caggcccatg gacagggcgc
    13741 acaccgccag gaaggcggcg atgaagcggc gccggcgcgg cagcagcacg ctcaggagca
    13801 cgatgtagcc cccccgcagg cgctcgaagg cggcgtcgaa gcgggtgtag aggggctgca
    13861 gccagccgcc cgccccgcca ccgtgctggc ccttcatgaa cagcagggcg agggtcggga
    13921 ccagggtgcg ggacagcagg taggaggcca gcatggcgaa gatcaccgcc tcggccaggg
    13981 gcacaaagag gtaacgggcc acgccggtga ggaagaacat gggcacgaag acgatgcaga
    14041 tgcacagggt cgacaccagc gccggcacgg cgatctcgcc ggcgccttcg aggatggcag
    14101 tctccaggtc gtggcccagg tgcaggtagc gctcgatgtt ctcgatggtg acggtagcgt
    14161 catccaccag aatgcccacc gccagcgcca acccgccgag ggtcatgatg ttgatggtct
    14221 cgccgagggc gtagagggtg aggatggagg ccagcaccga caggggaatc gagatggcga
    14281 cgatgcaggt ggtgcgccag ttgccgagga agagcaggat catcgcggcc gtgaggcagg
    14341 cggcgatgag gccttcgtgc accaccccgc cgatggccgc gcggacgaac acggactggt
    14401 cgaacagggg cgtgacgcgg atgtcctcgg gcaggctggg cagggcgtcg gccagggcgg
    14461 tgcgcaggtt gtccacgatc tgaagcgtgg aggcgccgcc gtttttgagc acggactgga
    14521 tcacgccgcg cttgccgtcc tggcgcacga tgttggtctg gggcgaaaag ccgtcccgca
    14581 ccacggccac gtcccgcaga tagaccgccg cgccgctgcc caggcgcacc ggcaggtcgt
    14641 tgagctcctc cacgctgcgc gtcgagccgt tcatcttcac cgcgaactcg gtggcaccga
    14701 tcttggccgt gccgccgggc aggatcaggt tctgggcgtt gatggcattg accacgtcca
    14761 gcggcgcgag ccccttggcc tgcagcgccg gcagatccag gtccacggac accacacggc
    14821 tcttgccccc atacggaaac ggtagcgcga cgcccggaat ggacaccagg cggggccgca
    14881 gggtgttcac cgcggtgtcg aacatcacct gctccggcag ggacgcgctg gagatgccca
    14941 gctgcaccac cggaatggcc gatgcggtgt agcgaatgac gaggggcggc gtgatgcccg
    15001 gcggcagctg gcgcagggcg ccgccctcgg tggacgcgat ctgggcgagg gccgcctcga
    15061 cgctggcctt gggctggagg aacagcttga tgatgacgat gccggcgtag gactgggact
    15121 cgaagtgctc cacgtcgttg accgtggtcg ccagcaggcg ctcgttgggg ccggtgacgc
    15181 gctgggccat ttcctgggcc gacatgccgg tgtaggtcca gatcacgctg atgaccggga
    15241 tgttgatctc gggcagcacg tccgtggcga tgcgcagcag caccagcggc gtggccagaa
    15301 cgatcagcag ggccatcacg atgaaggtgt aaggccgctg cagggcgatc cggacgatcc
    15361 acatggccta gctgcgggcg gaaagcggcg gagcagtcac gctgcccatg caggcgttgg
    15421 aggcggaacg gacgccgtcc gccgggaccg gcgcgggtcg gacgaccgga cgcaggggga
    15481 cgcccgcgac cgccgcagcg gaccggggaa gcgcatcaat caccaccggg ctgcgcccag
    15541 cgcgccgcag cgctgtcgtc ggcttcgcgg gcgtccaccc agcggccgcc cgcgggggtg
    15601 tcttcctttt tccagaacgg ggcgcgggtc ttgaggtagt ccatgatgaa ttcgcaggca
    15661 gcgaaggcgt cgccccggtg gctgctcgcc accccgacga agacgatgcg ctcgccgggc
    15721 agcagccggc cgaagcggtg gatgacccgc acgccctgca gggaccagcg cccggcggct
    15781 tcgtccacga tggcttcgag ggccttctcg gtcatgccgg ggtagtgttc cagggtcatg
    15841 gccgagacgt ggccggtctg gcaggcggac ttgtcggccc gcaccaggcc gacgaagctg
    15901 gccaccgcgc ccacgtcgtc gcgacccgcc gacagcgcgg cgatctcggc gccggtgtcg
    15961 aagtcggcct cctgcacctg gatgcgaaat cccgttgtgc tcatcctagc ctccggtcac
    16021 cggcggaaaa aaggcaatct cgtcccggtc ggctaccggg cgctcggggc cggccatctc
    16081 ctggttgatg gcagcgcgca cgttgcggcc ctcccccagc gcctcccaca caccgccgcg
    16141 gcgggccagg tgggtgcgca ggtcgcccag agtggcgatc tgcgtgggca gggtcagggt
    16201 ttcgtgggag tagccgaggg tttcccgcag gctggcgaaa tacaggactt tgacgctcat
    16261 gacagcagtt cggaataggg aatgtaatgg acccggtcgc ccgcctggac gacgtgaccg
    16321 ggaggattgt cgaccaggcc ctcggcccac accgtcgacg tgaacaccgc cggtccctgg
    16381 ttgggatgga tctcgacgcg cccgtcggca ccgagacggg cgcggaggaa ctcccgtcgc
    16441 cggtccggcc ggggcaggtc gaaggcggcc gtcaggggca gcgcgcgggg cagcacctcg
    16501 cgcaccccct gcgcacgcag gatgaaaggc cggaccagca ccaggaaggt gacaaagctg
    16561 gagaccggat tgccgggcag gccgatgaag gcggcgcccg ccgtcccggc ggccccgttc
    16621 acatgcccga aggccaccgg cttgcccggc ttcatggcga cctgccagga gtccaggcgc
    16681 ccctcggcct gcaccgccgg cttgatgtgg tcctcctcgc cggtggagac gccaccgctg
    16741 gtgaggatca ggtcgctcac ctccgccgca ccgcgcaggg cggcgcgggt cgcgtcgagc
    16801 cggtcgggca cgatgccccg gtcgtccact tggcagccca gtccttccag caggccccgc
    16861 agcgtgaagc ggttggagtt gtagatggcg cccggcggca aggcctcgcc gggcatgacc
    16921 agctcgtcac cggtggagaa cagggccacc cgcagacgcc gccggaccgt cagatgcgcc
    16981 gcgcccacgg aggccgccag accgaggcgc tggggcgtca gccggtcgcc ggcgtggagg
    17041 atggtctgac cgcgggcgat gtcctcgccg gcccgacgga tccattcgcc gggtcgcggc
    17101 aggtgatcga tcaccacgcc gtctccgtcc ggcgagcaga actcctgcat caccaccgca
    17161 tcggcgccgg ccggaatggg cgcgccggtg aaaatgcggg ccgccgtgcc aggctgcaag
    17221 ctgctcccca cgctgccggc cgggatgcgc tgactcaccg gcaggcgggt tcccggggcg
    17281 ggcagatcgg cgatccgcac ggcgtagccg tccatatagg aattgtcgag gggcggcacc
    17341 tccagcggcg acaccacggc atcggccagc acgcggccga gggcctcggc ggtgggcagg
    17401 acctcggtct cgacgacggg tcgggccagg gcgatcaggg cgtcgcgcac gtcctcgtag
    17461 gaacgcaggg ttgagctcat gtcagagttc caggtgatcc acgatgaagc cggccaccgc
    17521 ggccggatcg ttgagattga gacaaggcag atccgtcgca agtgccccgt cggtcgccac
    17581 ggccacgatg gccggatcgg acagatggag cagcggacgc ccgtgggccg gacgatggat
    17641 ctcgagcttg gggaggccca ggtgcttgaa tccctccacc agcaccaggt cgcagggcga
    17701 catgcgcgcc agctgggccg ccagatcggg ttccggctcg tccctcagct catgcatcag
    17761 ggcccagcgc ctttccgaga tcagcagcac ctcggcggcg cccgcctccc ggtgacgcca
    17821 ggaatccttg cccggctggt ccaggtcgaa gcggtggtgg gtgtgcttga tcaccgacac
    17881 cttcaggccc cgcccggtga gcagcgggat cacccgctcc atcagggtcg tcttgcccga
    17941 gccggaccag ccggcgaatc cgaacacctt catcccctca tcctcgggca tcgtcgcagg
    18001 gcaccgccag caccggcgcg aagccgccgc cgggggtgcg gaaattggtg gtctggcccc
    18061 gatacaggcg cgcggtcacg agctggacct ggcccccata cacgtagttg cgcaggtcca
    18121 tcttcagatc caccgccgcg ccatccacgg tcacgcgccg ctcggagggc ggcaccaggg
    18181 cctgggcgat gtaatcacca gccaggatct cggcaaagac ccgccgggtg agcttgtcgc
    18241 cccggtaggc gccgcggctg ccgaagcccg acgcgggctt gaagaagagg ccccgccgcg
    18301 ccgcccacag ggcctccgcc cggtctggcg tgaccgcctc ggtgcgcggc acgccggcgc
    18361 ccaggacggc ctgcgtctcg gcatcgacgc ccagcccgcg cagcaggtcc gggtcggaca
    18421 ggcggaccag attgcgcttg tccgcgtaga gggcgtgggc gcggggatgg ggtgtgatca
    18481 ccgcggcatc cgtgttccac gcggcacgca gggcggcgtg ggcaggttcg tcaagggcga
    18541 agtcggtgag gcggttgtag accatgtcca cccgctgacc gtcgtgccac aggcagcccc
    18601 gctcgcagcg cagctcggcc ggatcgcaga ccaccgcggc gatgccgtgg cgctcgaaga
    18661 ggcgctggaa gagcaaaaat tcgggcagca gatactggtc gccaggccga tcatccacga
    18721 tggcgatgcg ccgcaggggc gcgtcgcccc gctccgcctg ccattcggcg cggaacatgg
    18781 cgaggaaggc ggtttccgca tcgtcggtcc ccgtcggcac ggcggcgcgc atcaggctgc
    18841 cgacttccgc acagcaggcg cgctgggcac ggccgagcac agcgctgagc agtccgcccc
    18901 cggcattggt gttgatctcg atgagccgcg gtccgtcggg cccgaggtga aagtcgtaac
    18961 caaggaagac gccctgcgcc gcccctggac gccgcgccgc gacgggcgca tcggccagca
    19021 cggcagcctg ccaggcgggc tgcgcaacga cccgttcgat ggcggcgacc acctctgcca
    19081 tgtgctccag gtgggcctgg gcgacgaagg ccatggcgtc ggagaacagg tgcggacggg
    19141 attcggcgag gagcgcggtc agagccccgt cccgcggatc ccgcgccagg gcctcgcgca
    19201 gggccggcct gtcgagggtg atgcactggc aggtggcgtt cagggtttcg gcccgtccgc
    19261 cacagcccgc cgtgcccgta gagtgggccc ggtcggcggg ttcagggtcg gcggagcgca
    19321 gacgggtggt cacgaaagca acggtggggt cgggaaacgg gttcgggcga ttggcggatc
    19381 ggtacaggtg gcggattgta gcaagcggta gcgacgctca gcacccaccc gcggcaggcg
    19441 gcggcaggtc ttctagaaac gccaccactt cggcctccac cttgctgcgc cgcatcggcg
    19501 gcaggctctg ccagacacgc cggccgtagg gcttgctgag catgcgcggg tcgcagatgg
    19561 cgaggacgcc tcggtcgcgc tcggtgcgga tgaggcggcc ggcgccctgc ttgacgttga
    19621 tgacggcgcg cggcagctgg tactccatga agggattacg accgctcttc tggaggtgtt
    19681 cgatgcgcgc ggcgaggacc gggtcgtcgg gcggcgcaaa gggcagcttg tcgatgacca
    19741 ccaccgagag ggcgtcgccc ggcacgtcca cgccttccca gaagctctgg ctggccacca
    19801 ggatcgcatt gcccaggcgc cggaagcggg caaggagctc cgagcgggag ccttcgccct
    19861 ggagcagcag gggcagatcg agctgctcgg cggcgagctt gtcggcaatc acctcgtgga
    19921 tacggcgcat ggccttgagg gcggtacaca gcacgaaggt gcgcccctgg gcggcgcgga
    19981 tcaggggata ggcgacccgc gcgacggcct ccggatagac cgggctgttg gggtccggca
    20041 tgtcctgggg cgcatagagg atggcctgct gcgggtagtc gaagggactg ccccacaggg
    20101 cggtatcggg gggtggttcc agccagtgca ggcccagttc gcggcagtag tggccaaaat
    20161 cggggccgac ggcgagggtc gcggaggtga agatccaggc ccgtgggtgg ccgtccaact
    20221 ggcgcttgaa gatggaggcg atggacaggg gcgtggcgtt gagggccagg gtctggctga
    20281 agacctcgac ccagcgcacc cagtcggtgt tctccggcgc gcgccagcgg gtgaggcgtt
    20341 cctggatctc ctgcgtgcgg cgcaggcacg cggccagccc ttcggagcgt tcggcctggg
    20401 attcgacgag aatgcggaag gcacccagtt cgtcgtcgag atgcgccacc gcttcgtgga
    20461 agccggggtt gtcggcgagc tgggcaaagc cgaagcgggc gttctcggcg ctcaccgcga
    20521 ggcgcaggtc gcgggcagcc ttttcgaggc tgcgggtggc ttcgatgaga tccacgcagt
    20581 cccgcgcggc agcgacggtc tcggagcggg tatcgcgggc cagctccagc acctgggcag
    20641 tggacacgct ctcgccgaag aacaggctgg cggtctcggg cagttggtgg gcttcgtcga
    20701 agatcaccgc gttgcaggac ggcagcagtt cgcccatgcc ttcgtcgcgc agcatcacgt
    20761 cggcgaagaa gaggtggtga ttgaccacca ccacgtcggc ctccatggcc gcccgacggg
    20821 cggcggcgac gaaacattcc ttgatgttgg gacagtcctg acccagacag ttgtcccgcg
    20881 aagaggtggc gtacatccac gcgggcgcgt cttccggcac ctccgggcag tcggccttgt
    20941 cgccgctccg ggtgatttcc aggaagcggc cgatggcgcg caggtggacg gcgtcctggg
    21001 ggctctggaa gcgtccgtcc accaggttgc gcgccaggtg atagtgacac acgtagttgg
    21061 cgcggccctt gaggagggcg acggcaacgg gcaccttgag ggcgccccgc acggtgggga
    21121 gatcccggtt gaagagctgg tcctgcaggg tcttggtgcc ggtggacagg atgaccttgc
    21181 cgccgttgag aagggccggc acgaggtacg cgaaggtctt gccggtgccg gtgccggcct
    21241 cggccaccag cacccggttg gcgcgcaggg tgtcggcgat ctggcgggcc atctcgagct
    21301 gctgggggcg cggcgcaaag ccgggaatgg cggcggcgag ggggccgtcg gcggcgaagg
    21361 cggtgtcgat atccatgggc tcagtcgagg gcgtctaccc gcagccacac ctgccggctg
    21421 agcgtctcgc ggccctgcgc cgcgccagac agaccggccg cgttgcgagt ggattccgtg
    21481 gtgctgccac cgaggggaat ccaggccccc agagggccgc tcacggtggt cgcgaggcgc
    21541 gcggattcga cctgcccgcc agcgtccagg cgagcctgct cgggacggat ctcgatgcgc
    21601 acctgttcgc caacgagctg gggcgcggcg tagaaaccgc tgcccacgtc gacaagcgcg
    21661 atgctgtcgg ccgtcaccca gccaccgtcg ggcgtggcat agccctggcg catccgcagg
    21721 ggcacggact ggccgacccg gatcagggcc tgaccgccct cgatcacctc gacctgctgg
    21781 tcgatctgct cgcgggcgct gccctgacgg gcgtcgcccc ggatgacgac acggctgtcg
    21841 ccgggacgaa agatgccccc cacaccaccg tccgccatcg caaagtcccg cacatcgccc
    21901 tgacgcacgg aaatcaccag ccggcggagc ggcgtgtcga gggcttcgat ggcgcggcgc
    21961 agctcggcca gattagccgg cgaggtgcgc aggacgagct tgtcgcccat cgcgctgagg
    22021 gcgccgccgg gcgccacgag gggccggatc gccgccacga tctgatcggc ggggcgatgc
    22081 cgcagaggga tgatctccag gcgtccgacg ggcgcctggg ccgggacggc gggcacgccg
    22141 agcccgacga tggccgccat cgcgccccgc aagacggtga aacgcccacg ccccatggat
    22201 cgctcagtcg acccggtggg cctcggccag gtattcccgc gagcgcatct ccatcaggcg
    22261 gctgacggtg cgctggaact cgttggcctg atggccctcg cgatagaggt cgtaggcctc
    22321 gcattcggcg ctgaccagca gcttgacccg gtggtcatag agcacgtcga tgagccaggt
    22381 gaagcgccgc gcctcggagg ccatgccggc gttcatcttc ggcacgcggg agaggatcag
    22441 ggtgtgatgc tcgcgggcga tctccaggta gtcgttctgg gaacgggcgc ccccgcacag
    22501 ggtggcaaag tcgaaccaga tgacgccggg cgcctgacgt acgaccttga gcgtgcgctc
    22561 gagcacctcg atctcgccac cctcccccgg cgtgccggcg atctgggcga aatcggacgc
    22621 caggcgcttg tccgcgacat cgtcgccggg caccaggaag gcttccatct tctccagact
    22681 gcgcaggcgg tagtcggtgc cgtgatccac ctccaccacg tcgaaacgcc gcttgatcat
    22741 gtcgatggtg ggcaggaagt tgatgcgctg cagaccattg ggatacagac cgtctggcgg
    22801 gtagttggag gtcatcacga agatcgtgcc acgctggaac agggcgtcga gcagacggcc
    22861 gaggatcatg gcgtcggcga tgtcggagac gtggaactcg tcgaagcaca gcaggcgggt
    22921 ctgacgcgcg atgcggtcgg ccaccttctg caggggatcg ggctcgtggt tgaggttctt
    22981 cagatcgttc tgcacttcct gcatgaaggc atggaagtgc acccggcgct tgcgctggta
    23041 gggcaccgaa tcgaagaagc agtccatcag aaagctcttg ccgcgcccca cgccacccca
    23101 gaaatagacg ctgcggggca tcttgggacg ggacagcagc ttgcggatgg ccccgcgccg
    23161 tgcggccttg aaggccacca gttcggagta catgccctgc aggcgctggg cagcggcacg
    23221 ctgggccgga tcggacttgt agccccgggc gcggaggagg ccttcatagg cgtccagcat
    23281 gcccttttcg gaaacgttga ggatgcggtg cggcatggtc gagaccgtat ttccgggtga
    23341 ttcaatggaa cgggggcggg aggtaggcgc ctacctccca cccccggtgg tgcgggggcc
    23401 ggcacgggcc ggcccggtgt cacatcagaa gtgcagcgga cgcttgtcgc aggccagggc
    23461 cgcttcgtgc acgacttccg acagggtcgg gtgggcatgg cagatgcggg ccaggtcttc
    23521 ggacgcgcca gcgaactcca tcgtcaccac ggcctcaccg atgagctcgg aggcattggc
    23581 gccgatgatg tgcacaccca gcacgcggtc ggtgttggca tccgccagca tcttgacgaa
    23641 gcccgccggc gcaccggccc ccagggcccg gccgttggcc aggaagggga tcttgccaac
    23701 cttgtaggcg acgccttcgg ccttgagctg ctgctcggtc ttgccgaccc aggcgatctc
    23761 gggcgaggtg tagatgaccc aggggatggt gtcgaagttg gtgtgaccct tctgaccggc
    23821 catcgtctcg gccacggcca cgccctcttc catcgccttg tgggccaaca tggggccacg
    23881 caccacatcg ccgatggccc agacgcccgg caggttggtg cggttgtggc catccacgtc
    23941 cacgaagccg cgctcgtcga tcttgagccc caccgcttcg gcgttgaggc cggcggtgtt
    24001 gggcacgcga cccacggaga cgatcagtcg gtcgaactcg gccttctggg cgccatcctt
    24061 ggtctcgtac tcgacggtga cggacttctt gccgaccgtg acggcgccga ccttgacgga
    24121 cagcttgatg tccaggccct gcttggtgaa gagcttgagc gcttccttgg ccacatcctg
    24181 gtcagcggcg gccaggaagg agtccatggc ttccaggatg gtgacttcgg cgcccaggcg
    24241 gttccacacg gaacccattt ccaggccgat cacgccggag ccgatcaggc cgaggcgctt
    24301 gggcacggcg tcgatgtcga gcgcgccgat gttgtcgcac accagcttgt tgtccaccgg
    24361 aatgcccggc aggtggcggg ccgtggagcc ggtcgcgacg atgacctgct tggcctcgac
    24421 gacctcttca ccaaccttga cctgccagcc accgtcgatg ggggccacga aggaaccgtg
    24481 accggccagg aaggtgacct tgttcttctt gaacagaccc ttgatgccct gggtgagttg
    24541 ctcgacgatc ttggacttgc gcccgatcat ggtgggcacg tcgatcttgg gcttgccaac
    24601 ggagatgccc tgctcctcga aggcatgacc ggcttcttcg aacaggtggg acgtgtgcag
    24661 cagcgccttg gaggggatgc agcccacgtt caggcaggtg ccgcccagac ggggttcgcc
    24721 cttcgggtcg gcatagggat tggattcggc gcaggccgtg ctgaaaccga gttgcgcggc
    24781 gcggatcgcg gccacgtagc caccgggacc accgccgatg accagaacgt caaattgctt
    24841 cgccatgtga gagttccttg tgcggaggga aacaaggcac ggggcgacag gatcgctgtc
    24901 gcccgcgtgc cccggatcgg aagatcagac gtcgaggatc agacgggccg gatcttccag
    24961 cgcttccttc atggtcacca gacccagcac ggcttcacgg ccgtcgatga tccggtggtc
    25021 ataggacatg gcgaggtagt tgatcggacg cacgacgacc tgaccattct cgaccacggc
    25081 gcggtccttg gtggcgtgga tgccgaggat cgcggactgg ggcgggttga tgatcggggt
    25141 ggacatcatg gaaccgaaca cgccgccgtt ggagatggag aaggtgccac cggtgagctc
    25201 ttcgatggag atcttgccgt ccttggcctt ctggccgaat tcggcgatct tcttctcgat
    25261 gtcggcgatg gacatctgat ccacatcacg caggatgggc accaccagac cgcgcgggga
    25321 gcccacggcg atgccgatgt cgatgtaacc gtggtacacg atgtcgttgc catccacgga
    25381 ggcgttcagg atcgggaact tcttgagcgc ggcgacagcg gccttgacga agaagcccat
    25441 gaagcccaga cgcacgccgt gagccttctc gaacttgtcc ccgtactgct tgcgcagggc
    25501 catgatcggg gccatgttga cttcgttgaa ggtggtcagg atggcgtttt cgttcttgga
    25561 ctggatcaga cgctcggcga tacgggcgcg cagacgggtc atcggcacgc gctgttcgac
    25621 gcggtcgccg gtcggcacgg ccacggccgg cgcagcggca gcggccttgg gctgggcggc
    25681 cacggcgtct tccttggtca cacgaccacc acggccgcta ccggccacgt cagcggcagc
    25741 gatgcccttc tcgtccagga tcttgcgggc cgaggggctg gcggcggaga ccgcagcggc
    25801 cggcgcagcc gcagcgggcg cagcagccgg tgccggagca gccgcagcgg ccggcgcagc
    25861 agcggccttg gcttcagtgt cgatggtggc aatcacttcg ccggaggtga cggaatcgcc
    25921 accgttcttg acgatcttga ccagcacgcc gtcggccggg gccggggttt ccagcacgac
    25981 cttgtcggtt tcgatatcga tcaggttctc gtcgcgactg acagtgtcgc cttccttctt
    26041 gtgccagctc accagggtcg cttcggagac ggactcggac agttgcggca ctttgacttc
    26101 gatcagcatg ttttctccct cttgttcaat tcggcacgga catgagcatc aggcattgat
    26161 ttcgccgaac gcattttcga taacagcctt ttgttgcgca ttgtgcttgg cgtagtaccc
    26221 caccgccggc gaagcggctg ccgggcgcgc caccagcagg aacttgtggt cgtcgctcag
    26281 gttggtgtcc aggtgatggc gggacgccag ccagtaccag acgccctggt tacgcggctc
    26341 ttcctgcacc cacacgactt ccttcgcgtt ggggtacttg ttgacctcgg ccgagaaggc
    26401 atcggccggg aacggataga gctgttccat gcgcacgatg gcgatgtcgt cgatgttgtt
    26461 ggcacggcgg tgggccagca gttcgtagta gatcttgccg gagcacagga gcacccgctt
    26521 gaccttcttg gcatccagat cgtccacttc gccgatgacg gtctggaagg tgcccttgga
    26581 gagatcgtcc agggacgaca cggcatcctt gtgacgcagc agggacttgg gcgtgatgat
    26641 caccagcggc ttgcgcagct tacgcagggc ctgacggcgc agcaggtgga agatctgcga
    26701 cgggccggac gggatgcaca cttccatgtt catctcggcg cacagctgca tgtagcgctc
    26761 gatgcgggcg gaggagtgct cgggaccctg accttcatag ccgtggggca gcatcatgac
    26821 cagaccacac tggcgacccc atttggcttc gccagagctg atgaactggt ccatcaccac
    26881 ctgggcaccg ttggcgaagt cgccaaactg cgcttcccag atgacgagct ggttgggctc
    26941 ggcggaggcg tagccgtatt cgaaggccag cacggcctct tcggacagca cggagtcgta
    27001 gcagtagaac gggccctgac cttcgcggat gttcttgagc ggatggaagg taccatcgtg
    27061 ccactggtcg cggttctgat cgtgcagggc cgcatgacgg tggaagaagg taccgcggcc
    27121 tacgtcctca ccggagagcc ggacgccata gccctgcacc agcaggctgg cgtaggccag
    27181 gttttcaccc atgccccagt ctaaggccag cttgccttca cccattgcct tgcgatcgtc
    27241 gatgatcttc tggacgcggg gatgcagcac gaaaccttcg ggcgtggtgg tgagcgcctt
    27301 ggacagacgc ttcagctcgg ccatcggcac tcgggaatcg cacttgtcgg tgtacttctt
    27361 gttaacgtac gggctccagt cctgggagaa ctggcgcttg aagtcggaca gcacggggtt
    27421 ggtgacgagt tcgcccttgt ccagcgcggc gcggtaatcc ttgatgaact gatcgggatc
    27481 ctcggccttg cagacaccca gctgcaccag cttttccgcg tacagcttgc gggtgccggg
    27541 gtgcttggcg atcttcttgt acatcagcgg ctgggtcacc atcggctcgt cggcttcgtt
    27601 gtggccgagc ttgcgatagc agacgatgtc gatgaccacg tccttcttga aggtctggcg
    27661 gtattcgaca gccagacccg tcacgaaggc cacggcctcc ggatcgtcgc cattgacgtg
    27721 gaagatcggc gcctcggcca tcttgaagat gtccgtgcag tacagggacg agcggtagtc
    27781 gcgcggatcg gaggtggtga aaccgatctg gttattcacc acgacgtgga tcgtgccgcc
    27841 cgtgccatag ccgcgggtct gcgcgaagtt gagcatttcc tggttaacac cctggccggc
    27901 gacagcggcg tcaccgtgga tgatgacggg gatcacctcg gccttgccgt tctcaccgcg
    27961 ccgggtctgg cgggcataga cggagccttc gaccaccggg ttgacgattt ccaggtggga
    28021 cgggttgaag gccagggtca ggtgcaccgg accgccggac gtcatcacat cgctggagaa
    28081 gcccatgtgg tacttcacgt cgcccgcgga gagatcggac gcagccttgc cttcgaattc
    28141 ggagaacaac atcttcggcg acttgccgag ggtgttcacc agcacattga gacggccgcg
    28201 gtgagccatg ccgatgacga tctcggccac gccctgggag ccagccacgc ggatcagctc
    28261 gtccatcgct acgatggtgc tttcaccgcc ttcgagggag aagcgcttct ggcccacgta
    28321 cttggtgtgc aggtagcgct ccatcgtctc ggcagcggtc aggcgttcga ggatgcggcg
    28381 ctgctgttcc gccgggaagg cgggcttgcc gcgagtcggt tcgagacggg cctggatcca
    28441 gcgcttctcg gcaatgtccg tgatgtacat gtactcggcg ccgatggagc cgcagtaggt
    28501 ctgacgcacg gcatccagga tctcgcgcag ggtggcgtgg tcgccaggca gaccgtggaa
    28561 ggaaccggtg ttgtaggtct ggttgagatc accctcggtg aagccgtagt gggacagctc
    28621 gagttcgtgg atctcgggac gctcggtgcg cttgagcgga tcgagctgcg cccagcgggt
    28681 gcccaggaag cggtgggcgt tgatcagttg caggacgctg acctgcttct ggtcaccgcc
    28741 accggctgcc acggtacgca ccgggccgcg cttggccata tcggcgaagg cgttgatgat
    28801 cgggtaatgg gcgacatcgc ggccgttacc gggggtgttc tgcagcgtat cgaaatagct
    28861 gcgccactcg tcggagaccg aatcgggatt gtctagatag gcttcataga gctcttcgat
    28921 gaacggcgcg ttgctgccga agagctggga cgtgttgaaa agctgtttca tcatgggcga
    28981 ccacctgact tgtacgagtc ttatttattg gcttgggtat attccggtgg ggagaatccc
    29041 ttgtctgcaa ctgttcgcgg aaaaacaaag cgggatggcc tgatgatgcg accaccccgc
    29101 attgcctcgc cggtttcccc ctccccctcc ggctgcttag ccgcgctggg ccagaggcac
    29161 aacgtcgcgg cgcgcagcgc cgatgtacag ctgacgcgga cgaccgatct tgtattccgg
    29221 atcggtgatc atttcttccc actgcgtcat ccagccgacg gtgcgggcca gcgcgaagat
    29281 gcaggtgaac atctcggtcg ggatgcccag cgccttctgg acgatgccag agtagaagtc
    29341 cacgttcggg tagagcttct tctcgacaaa gtactgatct tcgagggcga tcttctcgag
    29401 ctccatggcg agcttgaaga ggcgatcgtt ctcgaggccc agttcggtca gcacgtcgta
    29461 gcaaaccttg cgcatcagct tggcgcgcgg atcgaagttc ttgtacacgc ggtggccgaa
    29521 gcccatcagc ttgaaggtgt cgttcttgtc cttggcgcgg ttgatgtatt cgccgacacg
    29581 ggacacgtca ccgatctcct cgagcatctt gaggcaggct tcgttggcac cgccgtgggc
    29641 cggaccccac agacaggcga taccggcagc gatacaggcg aacggattgg cacccgacga
    29701 accggccaga cggacggtgg aggtggaagc gttctgctcg tggtcggcgt gcagcgtgaa
    29761 gatgatgtcg agggcgcgaa cgagcgtcgg gttcggcacg tacttctcgc aggggttgcc
    29821 gaacatcatg cgcatgaagt tcgccgtgta atccagttcg ttgtccggat acatgaacgg
    29881 catgcccatg ttgtacttgt atgccatggc cacgatggtc ggcatcttgg cgatcagacg
    29941 gttgaagctg acgttgcggt gctcggcatc ggcgaagtcc atggcgtcgt ggtagaacgc
    30001 ggacagggcg ccgacgacac cggtcatgac ggccatcggg tgggcgtcgc ggcggaagcc
    30061 ggagtagaac ttggcgagtt gctcatgcac catcgtgtga cgcttgatgg tgttctcgaa
    30121 gtcgaccttc tgggtggtgt tgggcagttc accgtttttc agcaggtatg tgacttccag
    30181 gaagttgcac tgctcggcca gttgctcgat cgggtagccc cgatacagca gttcgccctt
    30241 gtcaccatcg atataggtga tcttggactg gcagctggcg gtggagagga agccggaatc
    30301 gtacgtgaac agcccggtct tcccgccgag ggtgcggata tcgatcacat cattgccatg
    30361 agtgcccgac atgatcggaa actctacgga cttaccatct acattcagtg cagcagtgcg
    30421 ttgcgtggtc atcttttggt ccctttatcc ctgttgaaac tccagctccc tagcggaaaa
    30481 actgctgcgt catccagcac gcagcagttt caccatttcc ttccagcggt cgtcggagac
    30541 cggcttgctg ccgttgacca tagcccacag gtcatggtcc tcgacaacca gcagactctc
    30601 cagatccgcc acttgatcat cggtgagatg atcgaaatgc gtatcgagaa agcgcgtgaa
    30661 caccagatcg agctccagca gagcccgtct gcactgccag cgcacgcgcc cccggttgag
    30721 cgtcatacgg cgcggcgcag catcatgtcc ttgatcttgc caatcgcctt ggtcgggttc
    30781 aagcccttcg ggcagacatc gacgcagttc atgatcgagt ggcaacggaa cagacgatac
    30841 ggatcctcga ggttatcgag acgctcgctg gtggcctggt cgcgggtatc cgccaggaag
    30901 cggtacgccg caagcagacc cgccggaccg acgaacttgt ccggattcca ccagaaggac
    30961 gggcagctcg tggaacagca ggcgcacagg atgcactcgt agaggccgtt gagctcctcc
    31021 cggtcttccg gggtctgcag gcgctcacgc tcgggtgccg gctcgttgtt gatcaggtac
    31081 ggcttgatcg agtggtactg cttgaagaac tgggtcatgt ccacgatcac gtcacggatg
    31141 accggcaggc cgggcaaggg acggatggtg atgggctgcg ccagatcatc gatgccggtc
    31201 agacaggcca ggccgttctt accgttgatg ttcatggcgt cggagccaca caccccttcg
    31261 cgacaggatc gacggaatga cagggaatca tccttggcct tcagtcggac gagcgcgtcc
    31321 agcagcttct tgtcggactc ttcgagttcg acggagatgt cctgcatgta gggctggttg
    31381 tccttgtccg gatcgtaacg atagatcttg aactgtacgg ttcgcttgtt catagtgttt
    31441 ctctccgggg cgctcagtag gtacgcttgg cgagcgcgat gggctcgact tcgggggaca
    31501 tcggctgcag gttgaccggc ttgtagtcga gtcggttgcc ttcgctgtac cacaaagtgt
    31561 gcttgagcca gttcttgtcg tcacggccgt tgggggtctc ggcgcagtcg atcgcgtcgt
    31621 cgcgcacgtg ggcgccacgg gattccttgc gggcctctgc ggacaccatg gtcgccttgg
    31681 ccacctcgat gaggttgtcg agctccaggg cttcgatccg tgcagtgttc cagacctggg
    31741 acttgtcctt gatctgggtg accttggcgc gctcggccac ttcaaggatc ttcgcggcac
    31801 cttccttcag catgtcggca aagcggaaca cgccggcatg cttctgcatg gtgcgctgca
    31861 tggcaatccg gacatcggcg acttcctcgc cgcccgtctg accttccagg cgggcaatcc
    31921 gcgccagaga gacgtcggcc gcgttgtcag gcagcggctt gaggggcaac tgaccggcct
    31981 tgaaatccgc caccacggtc tcgccggcgg acttgccgaa caccagcaga tcgagcaggg
    32041 agttggtgcc gaggcgattg gcgccatgca ccgaggcaca ggcacattcg ccggcggcgt
    32101 agaagccatg caccggcaca ttcgggttgc cgtcctgggg caccacgacc tgacccttgt
    32161 aattggtcgg gataccgccc atctggtagt gacaggttgg caccacgggg atcggggcct
    32221 tgatcggatc cactccagcg aactggatgg agatctcacg aatgcccggc aggcgcttca
    32281 tgatggtctc gggcgacagg tgggtgatgt ccagcagcac gtgatccttg tcagaaccgc
    32341 aaccccgacc ttcgttgatc tcggtgacca tggaacggga caccacgtca cgggaggcca
    32401 gatccttcag gttcggcgcg tagcgttcca tgaagcgctc accggacgcg ttacgcagga
    32461 tgccgccttc gccacgcaca ccttcggtga tcagcacacc tgcacccgcc acgccggtcg
    32521 ggtggaactg ccagaattcc atgtcttcca gcggaatgcc ggcacgcgcc gccatgccga
    32581 caccgtcacc ggtgttgatg aaggcgttgg tggaactgta gtagatccgg ccggcaccgc
    32641 ccgtcgcgaa gatggtggcg cgggaatgga agatcgagac ctcgccggtt tccatttcca
    32701 tcgcggtcac gccaaggaca ttgccttcgg catcgcgcag aagatcgagc gccatccact
    32761 cgacgaagaa ctgggtgttg gcccgtacgt tgcgctgata gagcgcgtga agcatggcat
    32821 gccccgtccg gtcagcggcc gcacaggagc gggacaccgg ggtctgaccg tagttctggg
    32881 aatgaccacc gaaggggcgc tggtagatct tgccgttgtc ggtacggtca aagggcatgc
    32941 cgtagtgctc aagctcgacc accacttcat tggccttttt gaccatgaat tcgatggcgt
    33001 cctggtcgcc cagccagtcc gaccccttga cggtgtcgta catgtgccag tgccagttgt
    33061 cctcggtgga attgcccagc gatgcggcca cgccgccctg cgccgccacg gtgtgggaac
    33121 gggtcgggaa caccttggtc agcacggctg tcttcatgcc cgcttcggaa agctggagcg
    33181 ccgcccgcag accggcgcca cccgcgccga cgattactgc atcaaatgta cgctttgcga
    33241 ctttcactta cagcctccac agaatctgag cagcccagcc ggcgtagccg atgagcagaa
    33301 gagcgacgac ggaatgcagc gtgagacgca gacccgtggg cttgatgtag tccatgaaga
    33361 tgtcgcgtac gccgatccac gcgtggtaga acagagacat gaagaacagg aaggtcgcga
    33421 acttcatgaa gccgccgcta aaaaggccgc gccagctttc ataggtgcca tccaaagtcg
    33481 ccagggcgga caccgcaaac agaatggagt agaccgccat gacgacggcg gtaatgcgct
    33541 gggcgagcca gtcacggaaa ccgtaatggg cgccgacaat aatgggcttt accatagttt
    33601 ggccaccagg atgacagtga gggcgaggct gacgatcagg acggtgcggg cactgctgac
    33661 cgcagattcc ttgtcgacac cgacatgcac gtccagcagc aggaaacgga tacctgcaca
    33721 gaaatgatgc aggaacgccc acagcaaccc gaccaggagc agcttgacga acacattgct
    33781 caccacgtcc ttgtaggccg cataggactc aggcgaaccg acgctcgcac cgaacagcgc
    33841 cagcaatacc ggcagcatca ggaacagccc cgcgccactg acgcggtgca ggatcgagac
    33901 tttcccgggg aggggaagcc tgatctcgtt cagcgccaga tgcttcgggc gctgtttctt
    33961 caaagtcacc tctgtcatag atagatactc cctctagcga atagcggctg agcgccgctc
    34021 ttatcccaca caacaaaatt gtaagtatat acccttacca gcccctgaca gaccgggcct
    34081 tgcgacccgc ccttcaactc agttcattga gataataatg gtctgccgtc gaatacaagc
    34141 cgcgccgcca ctccacgggc ctgtcgccat aggtgtaggt gacgcgctcg accgacagga
    34201 gcggcgtccc ttccgccacc ccgagcgtct cgcccaccat gcgatcagcc gcgactgcgc
    34261 ggatgcgttc ctcggcacgg atcatgcgga tcccgaaccg ggcctcgaac agcccgtaca
    34321 gggaccctgt cgaaccctgc agaacttcca tcgtcaggcc atgaaaaacc tcgcccggca
    34381 gatagatctc atccaccacc accggtcgcc cgccaaactt gaggaggcga cgcaggatcg
    34441 tgaccggcgc gccgagctcg attccgagca ttcgcgcggc ctcattgccg gccttggcac
    34501 gccagcaatc aagggggata ctttggggat gagccagctc accgtcgatt ggcacaagcc
    34561 gcaggaagcg gaacagcgca cgcggatcgt tgtgcgacgc gacatacgta cccttcccct
    34621 gcttgcgcac caacaggttc tcggcagcca tctcatcgat ggccttgcgc acggttccct
    34681 gactcacgct gaatcgcagg gccagctcct gttcgctggg aatggcctgc ccgggccgcc
    34741 attcagccga ctccaatgca ttcagcatca gattcttgat ctgacggtaa agggggctga
    34801 aagtgggcga gtcctgtcgc atggactaat tgaagcataa attcaaaaga cagtccatag
    34861 gctgtcttat atcttatata agacataaga catcgaaatt gacaggtcat caaaatgttc
    34921 ctacgattcg tatgttgacc gatccggcga tgatcgcaac ctcgacccat cttcgcggcg
    34981 gcgctacggc gacgcgaaat ctcgaagcat cagtccgata aaacttcttt ccttcctgag
    35041 ggagtatcaa taatggcaaa agcccccgtc cgtgttgctg tcaccggcgc tgccggccag
    35101 atcggttaca gcctgctctt ccgcatcgcc agcggtgaaa tgctcggcaa ggatcagccc
    35161 gtgatcctgc aactgctcga cctgccgcag gcccagaagg ccgtgaaggg cgtgatcatg
    35221 gagctggaag actgcgcctt cccgctgctg gctggcgtca tcgccaccga tgatccgaac
    35281 gttgccttca aggatgccca ggttgccctg ctggtcggcg cccgcccccg cggccccggc
    35341 atggagcgca aggatctgct gaccgaaaac gccaagatct tcaccgttca gggcgcggcc
    35401 atcggccagt acgccgatcc ggacgtaaag gtgctggtgg tgggcaaccc ctgcaacacc
    35461 aatgcctata tcgccaagga aatcgccgtc aagtacggtc gcgtccaggc caagaacttc
    35521 accggcatgc tgcgcctgga ccacaaccgc gccctgtccc agctggccac caagactggc
    35581 cgtgacgtgt ccagcctgaa gaacctggtc gtgtggggca accactcccc cacgatgtac
    35641 gccgactacc gcttcgccac gtccaacggc gacaaggtgc ccgccctggt gaacgacgag
    35701 gcctggaacc gcgacgtgtt cctgcccacc gtcggcaagc gcggcgccgc catcatcgaa
    35761 gctcgcggcc tgtcctccgc cgcctccgcc gccaacgcgg cgatcgacca catccgtgac
    35821 tgggtcctcg gctccaacgg cgaatgggtc accatgggca ttccgtccga cggctcctac
    35881 ggcatccccg aaggcatcat ctacggcttc ccggtgacca ccgtcaatgg cgaatacacc
    35941 atcgtccagg gtctggagat cgacgagttc tcccgcagca agatgaccaa caccctcaac
    36001 gaactcctcg aagagcgtga cggcgtcaag gatctgctcg gctgatcctc gctgcatccc
    36061 agctgtgcag aacgcggggg cggaaacgcc cccgcgtttt ttgttatcct ccggcggctc
    36121 agatgccctc tttccgcacg ccatgaccgc cgaccaccac cccgattccg tcctcttcca
    36181 gggcgaaaag cccttccccg tcctgccggc cgttgatcac tatgccggca gtgagaaact
    36241 gatgcgcaag gccctcgccc tgcagcagga gatcggtccg ctcttcgaca tcacttgtga
    36301 ctgcgaggat ggcgcccacg ctggcgccga ggccgaacat gcccgcatgg ccgccagcat
    36361 cgtcatgtcc ggcgacaacc gcttcggccg cgtgggggcc cgcatccacg acatcaccca
    36421 cccccactgg gaacaggaca tggacatcct cgtgggcgaa gccggtgccc gcctggcctt
    36481 cctgacgctg cccaagcccc gcagcgttgc ggatgccgcg cgccagatcg acgccctccg
    36541 cagcgccgag gaacgccacg gcatcgagca ggccattccg gtacacgtcc tcatcgaaac
    36601 ccacggtgca ctgcgcgatg cctgggagat tgccgccctg gaccgggtcg aatccctcga
    36661 cttcggcctg atggacttcg tcagcgggca ccatggcgcc attccgggcg ccgccatgcg
    36721 cagccctggc cagttcgagc atccgctgat catccgcgcc aagtgcgaca tcgccgccgc
    36781 cgccctggcc cacggcgtcg tgccgtccca caacgtcacc accgaactca aggatctggc
    36841 ctacatccgc cgcgacgccg aacgcgcccg tcgggaattc ggctacctgc gcatgtggag
    36901 catccatccc aaccagatcc tccccatcgt cgaagccatg cgcccggatt tttccgaggt
    36961 cgaggaagcg accgccatcc tcgcgggtgc ccaggatgcc ggctggggac cgatccagca
    37021 cgccggcaag ctccacgacc gggcgtccta ccgctattac tgggagctcc tcaagcgtgc
    37081 ccgcgccacc ggcatgaatc tgcccgatga cgccctcgcg cgcttcttct gagcactgcc
    37141 cacaggacat cggcccgatc actccctggc gcgagccgga agcgatcggg ccgattggca
    37201 tccgccgggg ccgatgccct caatccggca gggcaatctt ctggtcggtg ctgaactggt
    37261 agtcgcggaa gatgtgctcg gcagtcagta gccggtactg accattctcg tcgagccggg
    37321 tcgtatcctt caggcgatag gtgaagtgcc cgcaggtcca gcagtcgaag ttgcgcatgt
    37381 gggtgcacag gcaggtcttg tccagcaccg aaagtttctt ctcggccgga tgcagcgcca
    37441 gttcccggtt gtaggcctcg atgtaggcgc agctcccctt ggcgtcgagc agatagccgt
    37501 aggcctcaca gttggggcgg atgcccgaac cgatcgccgg actgttcttg agcatgcgca
    37561 tcggatagcc ggtgggcgag atctgattca cctcaatgtc atcctcatcg gcccggaaat
    37621 actgctgctt gactgcgtcg ggcagaccgc attcctgaga caccgtgaaa cgcgtggcga
    37681 cctgtacggc gccggccccc gcctccagaa aggccgccgc atcactgccg gtgaagatgc
    37741 cgccggccgg gatcagcgga atgtcgagct gctcgacctt cagccagtcg cggatctcgg
    37801 caacaatggt cgccagatcg tactgcgccc agtccatgcc gaaccccagg tgcccccccg
    37861 caagcgggcc ttccaccacc acataatcag gcagacgctg cgtgcgggca ctcttgcgca
    37921 ggaagagctg gagcgcgcgc agggacgaaa cgatgatgcc aagcttcaca tcgcggaagc
    37981 ggggatgatc ctcgatcaag gcaaacgagc ccagatgcag ccccgccgcc agggtgatgc
    38041 cgtcgattcc cgcgtccatg gccgcagtga ggcgcacctt gagggtttcc ttgggcgcgt
    38101 tcatcgtgag cttttccatg cagttcacga agatcagccc ctggccgcgc ttcgcctcca
    38161 tggtccgccc cacgtgcaga tgggtcgctt cggccaggtg gcccagatcg aactggacct
    38221 ccgatttgtc agggtttgca acgttgtact tgtattgctg aagtttttcc ttcacatact
    38281 tcgtcttgaa acggcgatcg gctaccgtct tcaccatcgc atccgagatg tggccgaccc
    38341 cccccagccg cgccgcttcc agcgcaagat ccgcggtgga gatgtcgacc cccatcccgc
    38401 cgatcatgat cggcacgtac tcctgcctgc cgaagcgcag ccggaaatca tcaactcgtt
    38461 tcatgaagcc ctttcaagac gtaaagagcc gcggacgatg cgcgtaccgc aggcgtaggc
    38521 gcgcattatc ccaccgccca cgacgttgcg ccgcagatta gatcaaggaa gtgccatctc
    38581 ccttgccgcc tcctttcgtc gcagcatcaa cgccgacaag cggagcagcc aaggccatca
    38641 aaatttggta cctttgaaac tgcggaattg ttcacggatt ccgtaaaatt ctgtgtcacc
    38701 caaacgtccg aatcgagatg cctgaagctg tacctcccag ttccgagtac gcagtcctgt
    38761 tcgccgatct ggtcggcagc acggagctct acgagcgcgt cggcgacaag gcagccttcg
    38821 aacaggtgga tcgttgcatc cgcggcatgc gcatcgccgt tgaaaccaag cagggacgtg
    38881 tcgtcaagca caccggagac ggcctcatgg cggtcttccg cgacgccgac agtgcagccg
    38941 atgccacgct gctgatccac cagaccgtca aggaacttcc cgcggccggt gaccagaagc
    39001 tggccttgcg catcggcttc cacttcgggc cggtcgtcgt ggccggcacg gatgtcttcg
    39061 gcgaaaccgt caacttcgcc gcccgcctgg cggagctggc ctcgcccgga aaggcgatca
    39121 cctcctcaga gacggcgcgc cgcctgggcc ccgagtggcg ctcggtgctg cataccctgc
    39181 cgccccgggc gatccggggg ctgtcgcggc cggtcgaggt ctgcgaattg atgtgcgagg
    39241 cggtgggtga gctcaccatt gtccagagcg atcatttcct gctcgccacc gaaccggaac
    39301 tgcgtctgta cctggactcc aagacgctcc tgctcaatgc cacgcggccc agcgcgcgca
    39361 tcggtcgcga caccgcagca gatctggtgg tggccgacac ccagtcgtca cgccgccatg
    39421 ccgagatcga actgcgcggc gacaagttcg tgctcatcga ccgcagcagc aacggcacct
    39481 tcgtacagat cgacggcgag aaggagttcc tgctgtcccg cgaagaagtg gtcctgcgcg
    39541 ggcgcggcca cttctcgctg ggccgcagct gcgccaacag cccccaggtg gtcgacttcg
    39601 tctgcctttg agcctgcacc cccggcaggc gtcctacgcc ttcaaggcat ccctcaaagc
    39661 ctcacccgcc cgcgtcacgg cctcattggc ctgcggaaag aacttcccga gggtgaagaa
    39721 gccatgcacc atcccgtcga acccatgcac cgaaacgggc acaccggccg cgcgcagtgc
    39781 gtcagcgtag gcgaggcagt cgtccgtaag cggatcgcag ccggcggtga tgatcgctgc
    39841 cggcggcagg tcatcgaagc gcggtgcgag cagcggtgag gcgcgccagt cggaccagtc
    39901 ttccgcattg ggcagatact tgtcgaagaa ccaggccatg ctctcgttgt cgaggaagta
    39961 gccgttcgag taggtcttgc gggacggtcg ctggctgagg atttccacac acggatagat
    40021 gagcagctgc agccgggcgg catggacccg ggcgttacgc atctggtggg caagcacggc
    40081 cgacagggtc cccccggcac tgtccccggc cagggcaaaa cgcgccggat cgacgcccag
    40141 caggaccgcg ttcgcaagcg cccaatccac cgcccgctgg gcgtccacca ctgcgccggg
    40201 gaagggatgc tcgggcgcca gcgcgtaatc caccgacagc accgcgcccc ccgacagatt
    40261 ggccagttgc cgccccagca cgtcgtagct cgccacgttg cccacgcacc agccgccgcc
    40321 atgaaaatag aaggtgatcg gtagagcctc gtcggcgtgg gaattcagcg gacggtacag
    40381 acgcgccatg atcacgccgt cgcgggcgtg ggcgcggggg atgggcactt ccgtcaccga
    40441 cgcgacgggc ggcgcctcgg gacgcagggc gaacagcagc ttctcgaagg aatgccgggc
    40501 ctgatgcacg tcgagctcgt ggaagcgcgg ggcacccacg cgatagacca tgtcgagcag
    40561 agcccgtgcc tggggatcga gagccatggg agaaggtccg gaaagatcga tgggagtggc
    40621 gaacgacatc atcgcgcgcc gcaggccaga atcagagcaa gcatcacgcc agacagccgg
    40681 gccagcgttg ccggcaatga tccgccccgg ccgacaccgg gtttgcggat gacgccccgg
    40741 acagctacca tgcgcgactt cgtccgatac gcgcgggcgc tgcccgacgt cggtgcgtcg
    40801 cgcaccgcat gactacccga cggcaaaaag cctgtggtgc cggaagaggg actcgaaccc
    40861 tcacgccttt cggcggcgga ttttgaatcc gctgcgtcta ccgattccgc catcccggca
    40921 agggagccgc gcattatccc cgaatcgact tactgcatca acatggcctt gaccctcgac
    40981 gatttcgact acccgctgcc ccccgaactg atcgcccagg cgcccctgcc ccggcgcacc
    41041 gacagccgcc tgctgcacgt ggccgagccc cttgccgacc tgcattttgc cgatctcccc
    41101 gccctgctgc ggccggacga tctcctcgtc ttcaacgaca cccgcgtgct tcacgcccgc
    41161 ctgttcggcg tgaaggacag cggcggacgc gtcgaagtga tgatcgagcg tcctctggac
    41221 gaacacgaag ccctcgccca gatccgcgcc agcaaatcgc ccaaagccgg cacccgcctg
    41281 cgcctggaag acgccctcga cgtggacgtg ctcggccgcg ccggcgactt cttccacctg
    41341 cgcttcccgc cggatgcgcc cctggtgacg ttgctggaac gtcacggccg tctgccgctg
    41401 cccccctaca tcgaacgggc ggccggcgaa gccgacgaat cccgctacca gaccgtctac
    41461 gcccgccatc ccggttcggt agccgcgccc accgccgggc tgcacttcga cgacgccctc
    41521 ctcgcccagc tcgccgctca gggcgtgcgc agcgccaccg tcaccctcaa cgtgggcgcc
    41581 ggcacctacc agccggtgcg cgtgcaggat ctgacccagc accgcatgca caccgaggct
    41641 taccacgtgc cggacgccac cgtcgccgcc atcgacactg cccgcgccac cggcggccgg
    41701 gtgatctgcg tggggaccac cagcctgcgc gccctcgaat ccgctgcccg cgacggcagc
    41761 ctgcgtgccg gccaggccga gagcgacctc ttcatcctgc ccggctaccg atttcgcgtg
    41821 gcagatgccc tcatcaccaa cttccacctg cccaagtcca ccctgctgat gctggtctcg
    41881 gcctttgccg ggatggacac cctccgcagc gcctacgccc acgccgtggc gcagcgctac
    41941 cgcttcttca gctacggcga cgccatgttc ctgacccgga agcccgcatg aaatacgaac
    42001 tcctcgccac cgacggcgcc gcccgccgcg gccgactcac cctcaaccac ggcaccgtgg
    42061 acaccccggc cttcatgccc gtgggcacct acggctcggt gaagggcatc tcgccccagg
    42121 acctgcgcga catcggcgcc cagatctgcc tgggcaacac cttccatctg tggctgcgcc
    42181 ccggcctcga cgtcatgacc gcctttggcg gcctgcacca attcatggcc tgggacggcc
    42241 ccctcctcac cgactccggc ggcttccagg tgttctccct gggtgccctg cgcaagatca
    42301 gcgaggaagg tgtgcgcttc gcctcgcccg tcaatggcga caagctcttc cttacgcccg
    42361 aggagtccat gcgcatccag cgcgtactca actcggacgt ggcgatgatc ttcgacgaat
    42421 gcacgcccta ccccgccagc gagaccgagg ctggcgactc catgcgcctg tccctgcgct
    42481 gggcaaggcg ttcgcgggac gaatttgacc gccttgagaa ccccaacgcc ctcttcggca
    42541 tcgtccaggg cggcatgtac gaatccctgc gcgacgaatc cctggccggg ctcaccgaga
    42601 tcggcttcca cggctatgcc attggcggcc tgtcggtggg cgagcccaag gaagacatgc
    42661 agcgcatcct ccatcacacc gcgccgcgcc tgcccaagga ctcgccgcgc tacctgatgg
    42721 gcgtgggcac gccggaggac atcgtcttcg cggtgcaggc cggcatcgac atgttcgact
    42781 gcgtcatgcc cacccgcaac gcccgcaacg gctggctgtt tacccgccac ggcgacatca
    42841 agatccgcaa cgcccgccac aaggccgaca cgcgcccgct ggacgactcc tgcgactgct
    42901 acacctgcag gcacttctcc cgcggctacc tgcaccacct caaccgcctc aacgagatcc
    42961 tcggcgcgcg tctggcgacc attcacaacc tgcactacta ccagcagctc atgcgcgaac
    43021 tgcgcgacgc catcgcccac ggccggctga cggactacgt cgcccgcttc catcgggaac
    43081 gggccggcca ggacgcctga acgcacgtgc cggcggcggc ttcgccgggc cgccccgagc
    43141 cgctataatc gcgcctttgc ccgaacgccc catgcaggtc ctccgcggaa tccccgacca
    43201 cgccgaacac ggctgcgtgc tcaccatcgg caacttcgat ggggtgcatc gcggccatca
    43261 ggcgctgctc gcgcggctcg ccgccaaggc gcaggagacc ggcctgcccg ccgtcgtgct
    43321 caccttcgag ccccacccgc gggaatactt cacccacgag gtccgcccgg tccgcctcac
    43381 ctccctgcgg gaaaaggtcg gcctgctcga acaacacggc gtggaccgca tcgtgatcgg
    43441 acgcttcaac gagcgtttcg ccggcctgag cgcggaggcc ttcatcgaag acgtgctgat
    43501 ccacggcctc ggcgtgcgcc acctgctgat cggcgacgac ttccgcttcg gccgcggccg
    43561 caagggcgac ttcgccctgc tgcgcgccac cggcgaacgg gccggcttca ccgtcgaggc
    43621 catgcccacc ctggcggacg ccgacggccg cgtgtccagc tccgccgtgc gcgaggccct
    43681 tgccgccggc gacatgcccc gcgccgcacg cctgctcgga cgcccctaca gcatcagcgg
    43741 acgcgtcctg cacggcgaca agatcggccg cacgatcggc tttcccaccg ccaacatcca
    43801 gctcaagcac cgcagcccgc cgctgatggg catcttcacg gtgagcgtgg acggtgttgc
    43861 agagcggccc tggcccggcg tggccagcat cggcgtgcgc cccaccatca acgacgccgg
    43921 ccggcccagc ctcgaagtcc atctcttcga ctggcacggc gactgctacg atgcccacct
    43981 gcgggtcaac ttcctcgcct ggcagcgcgc cgaagaacgc tacgacagcc tcgacgccct
    44041 gaccgcccag atcgcccgcg acgccgacca ggcccgcgcc catttcgccc ggaatcccct
    44101 ctgacatccc cgccgccatg tccgaactgc gcaagaccct gaacctgccc gacaccccct
    44161 tcccgatgcg gggcgacctc gccaagcgcg aacccggctg gatcgccgac tggcagcagc
    44221 gcaagctcta ccagaagatc cgcaaggccg ccgccggccg gcccaagttc gtcc
//
LOCUS       DAOQPA010000011        43173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_352_length_43173_cov_18.944478, whole genome shotgun sequence.
ACCESSION   DAOQPA010000011 DAOQPA010000000
VERSION     DAOQPA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 43173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43173
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_43173_cov_18.944478"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKX01_02900"
     CDS             complement(<1..225)
                     /locus_tag="PKX01_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75099.1"
                     /translation="MMTPALPHDWPWQPAPALPRTLRLALAASIGAHALALLGHYGLL
                     PNPTERPAPLQVELRLPPPSVAPASEPVRAA"
     gene            complement(222..1202)
                     /locus_tag="PKX01_02905"
     CDS             complement(222..1202)
                     /locus_tag="PKX01_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HMZ75100.1"
                     /translation="MSGAAPMRLRVPRLLRAPAAALALGWTLLLGAADFDYALQPRRI
                     ADDTWVLTGRNEDFTLANGGNIVNTAFIVTADGVVVIDSGPSRRYGEQLRQAIARVTD
                     QPIRMVLNTHHHPDHILGNQAFPADTLAALAGTADGIHTEGGAFNDNMYRLVGDWMAG
                     TEVVTPARRLPSGIQEIGGHRLEFIALDGHTGADLAVLDRSTGVLFAGDLVFHDRSPT
                     TPHARLPAWNAALDRLQASGFRVLVPGHGPVAGDAGPIRQTRAWLAWLDGTLRSAAED
                     GLDMNEVMALPIPAEFRRLAVVEAEYRRSVGQLYPAYEQAALHRPRRSGP"
     gene            complement(1199..1972)
                     /locus_tag="PKX01_02910"
     CDS             complement(1199..1972)
                     /locus_tag="PKX01_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HMZ75101.1"
                     /translation="MPRIITALMFALVCHAALAAAPADPPDPLNSGRWADMRKEVLPA
                     DARTVFDERIRVIAPISAENPLNVPITVDASALDGVEEVRVFADYNPIVQIVRFEPGG
                     ALATLGFRVKLQQSTPIRAAARGADGLWHVGGSWVNTTGGGCTLPSTGSSSPEWQRRL
                     GEVSSRVWARKEGGERIRLRIVHPEDTGLAAGIPAFYIEDLRLTDDGGRPLMRVRGFE
                     PIAENPVFTVDIPAAHTGHGYRINGRDNNGNTIAAEIDK"
     gene            2467..4233
                     /locus_tag="PKX01_02915"
     CDS             2467..4233
                     /locus_tag="PKX01_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HMZ75102.1"
                     /translation="MKKPFAMTVIAAAVMVAGAAHAKEVTDADILNDAKTPGDVVHFG
                     LGQQGQRYSALDQINTKTVKDLVPVWSFSFGGEKQRGQESQPVVFDGKMYVTASYSRL
                     FALDAKTGKKLWKYEHRLPEGIMPCCDVINRGAALYDNLVIFATLDAQLVALNKDTGK
                     VVWKEKIDDYAAGYSASAAPLIVKGLVITGVSGGEFGVQGRMEARDAKTGKMVWMRPT
                     VEGHMGYKWVNGEKVENGISGTTNATWTGDMWKTGGAATWNGATYDPETNLVFAGTGN
                     PAPWNSHLRPGDNLYSSATVAIDPDTGKIVWHYQTTPHDGWDFDGVNEFVSFDYKDPK
                     SGKVIKAGGKADRNGFFFVIDRTNGKLLNAFPFVKDITWAKGIDLATGRPIYNDENRP
                     GDPSAGTDGKKGSTVFAAPSFLGGKNQMQMAYNPKTGLFYVPANEWGMDIWNEPVSYK
                     RGAAYLGAGFTIKALHDDYIGALRAVDPSAGKIVWENKNFAPLWGGVLTTGGNLTFYG
                     TPEGYLKALDAKTGKEVWSFQTGSGVVAPPVTWTEGGEQFVAVVSGWGGAVPLWGGDV
                     ARRVNFLEQGGSVWVFKLHKSK"
     gene            4360..5700
                     /locus_tag="PKX01_02920"
     CDS             4360..5700
                     /locus_tag="PKX01_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ75103.1"
                     /translation="MKISGVPSMDLRWKLFGTVLGFALALLTAAAAWIGGALQRDATQ
                     EMAASARLVDAIRAASAAPGMRVDELERLLGAEPLRHVRLQIERSNLEHSVSDAPAGP
                     MDLLAAALLGERTVPEQRIPLADGVLVVRADAHAEIREILWDGAGLMLTLLVFSVAMA
                     VVAWLAAHRALKPVRELEAGLARIARDTGPVRLPEFELREFSRIGVAIERLAGDLARE
                     RADRQTLARRLVELQEAERRELARELHDEVGQALTAMGVRAAFIERHAGRADAVRLSA
                     CARDIRTESGRLVKLVGTVLARLRPRGLDDGAQVAAALDGLVRTWQHRAPEVAIEADL
                     PARLPALPPSAGLALYRTLQEALTNALRHSLADRVTLSLRADADAIDLVVSDNGGGRA
                     TDVAARGRGGLLGMRERAEMAGGRVWLSDGPDGGLSVRLSLPLHGGDRVPVPAV"
     gene            5747..6127
                     /locus_tag="PKX01_02925"
     CDS             5747..6127
                     /locus_tag="PKX01_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ75104.1"
                     /translation="MNALCKAALLAGLAAVATTASAAADDEMKALATKSGCLTCHAIE
                     SGTPGPNGMAPIGPAWQDVAKHYKGKPGADAFLTRVVLEGSNPYGSHWKDQVSGLAMP
                     PNKVAIKEADAKKLVKWILTLSPK"
     gene            complement(6254..6658)
                     /locus_tag="PKX01_02930"
     CDS             complement(6254..6658)
                     /locus_tag="PKX01_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75105.1"
                     /translation="MKIRPTSRPRRPTLATLCLVPLCALALNACSGRSDLPERLRQEF
                     RPGLSIAEAREKLTAHGTTYSIRNTMECEGLVRNSPIAALQPPQGGLCIFGKIPVSKS
                     WFGVRSDVILQLVFSGDGVLQDGNFEEVTSAQ"
     gene            complement(6720..8585)
                     /locus_tag="PKX01_02935"
     CDS             complement(6720..8585)
                     /locus_tag="PKX01_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMZ75106.1"
                     /translation="MSPIDQHLINLPVDTIRRSWERCATRGLRADERLPDDPNARVRL
                     SDRLEANARLVAYAQPIMEHLHQQIARSSSTILLADDSGVILRSVGDSDFLERAARVA
                     LKPGHCWSEHVMGTNAIGTALTEQRPVAVIGEEHFLDRNKFLTCIAAPIHAPTGGVLG
                     ILDISCSARLTQVHALALLQTTTEIIENRLIETLDDASVVLRFHHQPEALASPLEGLA
                     VFNESGGMLACNRRAERLLGLPDTRHSQSAFNDIFETRWNVLLDHALRSSFRPTKLRC
                     NRGREFAATVLVSNLRRVRPAGAVRDPRVLPRPRPRLTLEDMDLGDKAVGTAIRRARR
                     ILGLDIPLLIQGETGTGKEVFAQAFHNSGPRRDGPFVAINCAAIPGNLIEAELFGYTA
                     GAYTGARGEGARGKLREAHGGTLFLDEIGDMPLKLQAVLLRVLETRRVTPLGGGPEEE
                     IDLSLVCASHRSLKALCEVGEFRSDLYFRLSAMSLALPPLRERSDLEGMARRILQEES
                     PDRPIRLATSTLDLIRRHPWPGNIRQLKNALRLGVAMLGDDEETLGPDHLPQDLLDGD
                     DTVHPVPAAKSLRAAEVRLVEDTVQRHNGNISAAARELGITRTTLYRKLRQTSDD"
     gene            9018..9332
                     /locus_tag="PKX01_02940"
     CDS             9018..9332
                     /locus_tag="PKX01_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ75107.1"
                     /translation="MKFVQTVCAIALGCAVVSPALASEAIIKKARCVACHSVEEKRVG
                     PAYKDVAAKYKGQKDAEAALIAKVRNGGSGNWGQIPMPPHGPDKISDADLKAAIEWIL
                     KL"
     gene            complement(9496..10437)
                     /locus_tag="PKX01_02945"
     CDS             complement(9496..10437)
                     /locus_tag="PKX01_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ75108.1"
                     /translation="MRLTLSGRLLLACTAFPGLVLAAPFAYVPNEKSATVSVIDTATD
                     SVVDTLPGGKRPRGIATDGQRLYISEAPSSSLVVVDLARRQLLRSIRLGESPEGVSVS
                     TDGRLIAAAVEESNSVSLIQAATGKVLADIKVQGKNPEHAVFSPDGQWLLVSAEEAEQ
                     VDLIDVAARKQVASIAVGRRPRGIGFLPDSRRAYVACELDSTVYALDVTTRQATATIK
                     TGTFSNGIAVAPDGSRVYVSNGKDGTVSAIRTADNQVEATIPVGKRPWNMALTPDGRK
                     LYVANGKSNSVSVIDTTRLENIGDVPVGELPWGVVIR"
     gene            complement(10445..11293)
                     /locus_tag="PKX01_02950"
     CDS             complement(10445..11293)
                     /locus_tag="PKX01_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ75109.1"
                     /translation="MPTDRRQFLTAAGACLLAAGLPARADGLEDIRRRGSLTVAVYND
                     FAPFSSKGKGIDVELGEALGRKLGLATQVIGYNADEDMDDDLRNMVWKGHYLGARPSD
                     VMMHVPVDSYLQGKNDKVRIFGPYHLETIAVARDPARVGAFRGSAANALELFTREKIA
                     VEGRSLSDAFLLGVLNGRLRDNVVHCKDIAEAIEKLKAGEVIAVMGPRSEIEVALGKG
                     TRFIVDIVQMPELKINNWPLGMAVKADEAPLADALAGALKALEQDGTVAEIFARHGVT
                     YLKPTV"
     gene            complement(11283..11720)
                     /gene="pedF"
                     /locus_tag="PKX01_02955"
     CDS             complement(11283..11720)
                     /gene="pedF"
                     /locus_tag="PKX01_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HMZ75110.1"
                     /translation="MIQVTVRKSSLFRLATAAALSLAAGALFAHGDVTPQAVDTSTLK
                     PVGKEWLAKNPYSKDKEAIRIGSSAFNQNCARCHGLGAVSGGIAPDLRYLPEGQEGDE
                     IFLNRIRHGATRDGRVYMPPFEGILSQEAMWTIRAWLETVRAD"
     gene            12092..14158
                     /locus_tag="PKX01_02960"
                     /pseudo
     CDS             12092..14158
                     /locus_tag="PKX01_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            14349..18026
                     /locus_tag="PKX01_02965"
     CDS             14349..18026
                     /locus_tag="PKX01_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HMZ75111.1"
                     /translation="MWQKVHWNGPWARGAKWGGGVLAVVGVVGFLVAPPVVRSVAESA
                     LTQALHRPVSIEGVSINPYALSAEVRGLRIDEPGGGVEAASFKALYANLEAESLFRGG
                     VVVKEVQLIEPHVSVIRTEGQRYNWSDLIDEILNKPDDGSKTFFSVHNIRVEHGRVDF
                     DDRPTGQKHTLADLNLGVPFVSNLPSQVETYVEPLLAVTLDGAPFEVRGKARPFSKAR
                     ESAVELRFEGVDLTPYLAYVPLEKPFRVPSARLSSNLVVTFAQPDGQAPDVAVKGEVG
                     LDAFELQDAAGHPAVKLPHVAVGIERLAPLAGVVSLGTVTVERPEVVVDRDRDGRLGL
                     LKLVPKPAPSSEPPSPAAAPSDTPPARPLAVTLGEFRLTGGKVMISDALPGGTYRRTL
                     DDLHVSLRKFDLAGAEPAALDAGFHTDLGESVEHVGTVGLAPLRAEGTITVDRLDLPG
                     HAAYYAAALKQADVVSGHFDGKLAYAFSIEKEAPRVSLKGEVLGLKDLVVRLKGEKAP
                     LAKVGTLELRELAVDTAARKVDVGEVIGKATRLALIRDGEGRFNAERMTSTEAVPPPA
                     PEKPVRAAASKAPKAVAAGPAWQVAVRRVTLDDWGVRIEDQTLSPAIVFNAEPLSLRV
                     EGLSTAKGSKAKVELKASINKRGRFGVAGTVGLAPLAAALDLDLSAVDLAMLQPYVTE
                     KVNIAITRGVVTSKGRLAFELPDGGAMKGGFKGSLTIGDFASVDKLNAADFLKWRSLY
                     FGGVDVRLAPLAVNVDEIALTNFYTRLILDEQGGLNIREITAERARDQKRAKDAARAA
                     GKGSNAGSSAPPAPVAAKAVAAPAAPTPPPPVTIRRITLQGGNIAYSDRFVKPNYDAN
                     LTGMGGVLSGLSSDPSTLAELDLRGRVDNAAPVEVSGRLNPFRQDKALDIRASVTDFE
                     LSGVSTYAAKYVGYGIEKGKLSAKLNYKVEDRKLTASNQVFLDQLTFGDQVDSPDALK
                     LPVQLAVSLLKNSRGEIDLDLPISGTLDDPQFSVGGIIVKVIVNLIGKALTSPFALLG
                     SLFGGNAEELAWLEFDPGFARIDEKGESKLKAIAKAMSDRPGIKLEISGRVDPDADRD
                     GLRRAAMLGRVEALKVKEMARKGEAAGEDGRVRVDPAEYPVLLARVYKDADFVKPRTM
                     LGLTKDLPVPEMEKLLIANAKVTDEDLLTLAHQRAQVVKSWLIEQGKVAGERVFILAP
                     RVGKGGKDVSATLSRVDFSMR"
     gene            18046..18336
                     /locus_tag="PKX01_02970"
     CDS             18046..18336
                     /locus_tag="PKX01_02970"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMZ75112.1"
                     /translation="MNRNRLDAVQIDAALAELPGWQRVEGRDALGKTYRFADFNAAFG
                     WMARVALMAERLDHHPEWANVYSRVDVVLATHDAGGVTAFDVALARFMESAV"
     gene            18538..19470
                     /locus_tag="PKX01_02975"
     CDS             18538..19470
                     /locus_tag="PKX01_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75113.1"
                     /translation="MHLNLSFDLSAAVRRVRRPASVADASAIGAKVWKSFPYGNRLYR
                     YDGAALQANWARLHRGDREPFPSATRIERLVRHNPALAEHVRDPARTAACLEQAWRAY
                     HAGDFAESVHFAQDAGVAGTMVAVRAAAVYAAHVEDQDDRRLKILREATEAAEELVQV
                     APVWANAWYAFGLAVGRYSQNLSVMKALTLGFGGKVRDSFLRALALEPDHADAHLALG
                     VYHAEVVDKVGSVVGALSHHARRDAVEHHFELARRLTPDSPIVLAEYAKARMLAFGAA
                     QFGRAYGLFQEAAACRPVDALERLDVELAKRELI"
     gene            complement(19507..19953)
                     /gene="smpB"
                     /locus_tag="PKX01_02980"
     CDS             complement(19507..19953)
                     /gene="smpB"
                     /locus_tag="PKX01_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMZ75114.1"
                     /translation="MSIIDNRKAYHDYFIEDKYEAGLVLEGWEVKAIRAGRSNIKESY
                     VVIRNGEIFIVGMHITALESASTHIHPDPTRTRKLLLHATEIDKLIGKVERAGYALVP
                     LDLHYTKGRIKALIGLAKGKKQFDKREAEKERDWEREKARLIRAKG"
     gene            20021..20458
                     /locus_tag="PKX01_02985"
     CDS             20021..20458
                     /locus_tag="PKX01_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HMZ75115.1"
                     /translation="MAAVKKIVLIEFTPAQMFDLVDRCEDYPRFLPWCGGSEVHHRDE
                     RITRATLHINYHGIKSHFSTENDKQYPTHMHIRLTEGPFTHLDGSWDFTPLGDAACKV
                     EFNLRYEFSSRLIEKAVGPVFNHIANTFVDSFVKRAGQIYGGK"
     gene            20460..20789
                     /locus_tag="PKX01_02990"
     CDS             20460..20789
                     /locus_tag="PKX01_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HMZ75116.1"
                     /translation="MSDSINVEVCYALQDRQEIVSLRLPAGSTVQKAVEASGLLQRHP
                     EIDLVKANKLGVFAKLVKGDTVLRDKDRVEIYRPLIADPKEVRRKRAEEGKVMKKGGG
                     SADEAAA"
     gene            complement(21082..21540)
                     /locus_tag="PKX01_02995"
     CDS             complement(21082..21540)
                     /locus_tag="PKX01_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HMZ75117.1"
                     /translation="MSRALLALLILGASPLVLAQSIYSWKDAAGITHYSDTPPADSTS
                     RRLKQAPLAPPPTAQDGATQSFADKELAFRKRRAEAAEAEDKARKDKARDEDRQRECA
                     AAKSQLAGLESGQRIARFNAAGEKEYLDDAQRDAEIERARRVVENNCKPQ"
     gene            21624..23084
                     /gene="guaB"
                     /locus_tag="PKX01_03000"
     CDS             21624..23084
                     /gene="guaB"
                     /locus_tag="PKX01_03000"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMZ75118.1"
                     /translation="MRVIQKALTFDDVLLIPAHSSVLPRDVSLRTRLTRNVCLNIPLA
                     SAAMDTVTEARLAIALAQEGGIGIVHKNLTPQQQAAEVAKVKRFESGILKDPIIIPPT
                     MTVREVLTLTRTHKFSGLPVVDNGKVIGIVTNRDTRFETNLDQPVSAIMTPQARLVTV
                     REGASLDEARALMHKHRLERVLVLNEAGGLAGLITVKDMMKSTEHPLAAKDDLGRLRV
                     GAAIGVGAGTEERAALLAEAGVDVIVVDTAHGHAQGVLDRVNWVKKNFPKVEVIGGNI
                     ATAAAALALVEAGADGVKVGIGPGSICTTRIVAGVGVPQITAVDNVARALEGSGVPLI
                     ADGGIRFSGDISKAIAAGANVVMLGGLFAGTEEAPGETVLFQGRSYKSYRGMGSLGAM
                     QQGAADRYFQENDGNADKLVPEGIEGRVPYKGAVTAVIHQLMGGLRASMGYVGCATVD
                     EMRTRAEFVEITSAGMRESHVHDVQITKEAPNYHVD"
     gene            23557..24261
                     /locus_tag="PKX01_03005"
     CDS             23557..24261
                     /locus_tag="PKX01_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75119.1"
                     /translation="MLTRTVPLCASSASVVVEVAALSRRVVSEGAALTILDDVSFSIA
                     AGEAVAIVGASGSGKSTLLGLLAGLDVPDSGHVRVRGQDLFALDEDGRAALRGASIGF
                     VFQSFQLLPALTALENVMLPLELAGLPDARAAAKGWLERVGLGGRLGHYPRQLSGGEQ
                     QRVAVARAFAPQPALVLADEPTGNLDAATGEAVIELMFALNREAGTTLVLVTHDTALA
                     ARCGRVLRLAAGRLVS"
     gene            complement(24280..25590)
                     /gene="hpnE"
                     /locus_tag="PKX01_03010"
     CDS             complement(24280..25590)
                     /gene="hpnE"
                     /locus_tag="PKX01_03010"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HMZ75120.1"
                     /translation="MTPSVAIVGGGYAGFAAAVTLARGGARVTLFEASRVLGGRARVV
                     ETDGQRVDNGQHILLGAYSETLRLLRLVGVRPSVLATRRLALVYPGHCELRAAALPAP
                     LHLALGLLRARGLGTADKLAMLRLMRHLKRQRFRVAPDRSVGTLLTETAQTERLVRLV
                     WEPLCVAALNTPVADASAQVFAHVLRDSLAGSASASDLLLPRVDLSELFPVPASRWLA
                     LRGHTVRIVEPVKAIQRDDTGFRLDGDPFPTCYDHVVIATAPYHVAALVTGLPGMRPV
                     VTQIERLRHEPIVTAWLGFDGPVSFPEPMIGLSGGYGQWAFDRTALGGPTGLVSIVIS
                     ASGLHQQLDRDALRDALVAELAAALGPLPRLAWSRLITEKRATFACTPGLERPTPRTP
                     EPGLWLAGDYVASDYPATLESAVRSGVAAGRAILNACGLKESLF"
     gene            complement(25587..26423)
                     /gene="hpnD"
                     /locus_tag="PKX01_03015"
     CDS             complement(25587..26423)
                     /gene="hpnD"
                     /locus_tag="PKX01_03015"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HMZ75121.1"
                     /translation="MTDFHQYCQDKAAASGSSFYYSFLFLPPERRRAITALYAFCREV
                     DDVVDECNDPQVAQAKLDWWREEVHRVFDGAPHHPVGLALKDIASHFNLPREQLLEII
                     DGMQMDLVQTRYLDWKALHLYCYRVASVVGLLSAEIFGYTERQTLKYAHDLGLAFQLT
                     NIIRDVGEDARRGRIYLPVDELQRFNVPAHEILEARYSENFRSLMAFQAQRARGLYEQ
                     AFAQLPAADRKAQRPGLIMAAIYRSLLDEIEADGFQVLDRRTSLTPLRKIWIAGRTWL
                     RG"
     gene            complement(26429..27250)
                     /gene="hpnC"
                     /locus_tag="PKX01_03020"
     CDS             complement(26429..27250)
                     /gene="hpnC"
                     /locus_tag="PKX01_03020"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HMZ75122.1"
                     /translation="MPVDHYENFPVASFLLPARLREPVEAIYAFARSADDIADEGDAL
                     PVMRLARLNDYRRALHAIEQGTPYDDPGLAPMFDRLARAIRQFGLPVGLFRDLLDAFS
                     QDVGKTRYADFAELADYCRRSANPVGRLLLCLYRADTPENLRRSDWICTSLQLINFWQ
                     DVAVDLKKGRVYLPQDEMSLFGVCDEDLARGVVTPAVASLLDFQVTRARRMMTDGAPL
                     ARALPGRIGWELRLVIQGGLAILKKIEDVRYDVFRQRPTVGRTDWLRLAWRAAFR"
     gene            27356..28009
                     /locus_tag="PKX01_03025"
     CDS             27356..28009
                     /locus_tag="PKX01_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75123.1"
                     /translation="MLEIDHLCRSFQGRSVLRDVCLRLQPGDYVAIVGASGVGKSTLL
                     NLIAGLDRPDGGRLSLDGVAYDALDEDALTRLRRARFGFVFQAFHILPHLTLRQNVAL
                     PLWLQGRGGAEADAAAWALLEEVGLGARADGWPRELSGGELQRVAIARALVHRPRLVL
                     ADEPTGNLDPDNGARVLSLLAERVRASGAIGVLVTHSAEAAASADRVLRLTPDGLTG"
     gene            28006..30504
                     /locus_tag="PKX01_03030"
     CDS             28006..30504
                     /locus_tag="PKX01_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ75124.1"
                     /translation="MSVALRLAPVFLGALARRRMTTLLSCAAIALGVALGMGVQVIHE
                     AALAEFGRGLRSLAGAADLQVVGPRDGFDEALYGVLAARPEVAAANPLVEVDVRPAGR
                     DETLAVLGVDVFAAAEVAPALMPQADGGAGRLGVLAEDAVFLSAAAAEAWGAGATLTV
                     QAGAATLSLRVAGSVPGVGAGRRLAVMDIAAAQRLFGRLGRLSRIGLRLAEGVSPAQA
                     QRALAPLLPAGVILEVPAAEETRSASLSRSYRVNLALLAAIALLTGGFLVFSAQALSV
                     VRRRREFAFLRALGLSRRSLMAWLVGEGAIIGGVGGVFGVALGLGLAWMAVQALGGDL
                     GAGYFQGVAPRLIVDPLTCAGYAALGVAAGALGAWWPAREAARVAPAQALKAAADEAL
                     PGGSGRPWAALTLWCLAAAACLLPPIGALPIGGYGAVAGILAGAILFLPTIARVLSRC
                     LPRPRSVAGRLAVARLVAVPGHVVVAAAGVLTSVALAAAMAIMVTSFRESVDAWLTQL
                     LPADIYVRAGRAGAAGVLDEALQARLAALKGVVDVRFTRHEQLRLDGRGAPVALIARP
                     VSADGAGLPRVGGPRVPSGAPGLWISEAMADLYGWQPGMTVTLPLAGRAVTLPVAGVW
                     RDYARQTGSVLLDLEAYRRFTGDRRVHDAALTLAPGASAPDVARAVAALAPEGSLEVS
                     RPGEIRAITLGIFDRTFAVTYLLEAVAVLIGLAGVAASFAALAAARRREFGMLRHLGV
                     SRREIAAMLAREGALAAGVGVLGGLLAGGAIALVLIEVVNRQSFHWSMDLHVPWGSLA
                     VFALTLVALAALAAVAAGRQAMRREAVWAVREEG"
     gene            30669..31610
                     /locus_tag="PKX01_03035"
     CDS             30669..31610
                     /locus_tag="PKX01_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HMZ75125.1"
                     /translation="MTGWLTLPDGQPLGFQTTFFRVRTGIGEDNPSAFAPQQLVLAHG
                     ALADPRLGHLRHAERVARMGFGRAGFETSACRVWVGDWRFELDGGRYRARVRGEGFAY
                     ALDFEPDGAPVLNGVAGFSAKAADPRHASYYYSRPQLAVTGQVTREGVAQRVKGLAWL
                     DHEWSSELLPTGAQGWDWIGLNFDDGTSLMAFRLRASRGEPLGASATLRTRTGVHTVP
                     ATAVAFEPLRTWTSSRTGIRYPVAWRVRVADRLIELQPLLDDQELDSRRSTGAVYWEG
                     AVRALEGGRETGRGYLEMTGYGDAIRLGARGEEDGSA"
     gene            31738..32079
                     /locus_tag="PKX01_03040"
     CDS             31738..32079
                     /locus_tag="PKX01_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HMZ75126.1"
                     /translation="MGERYRVHIENSGEDYVCPTEQSLLRGMEVLGRRGIPVGCRGGG
                     CGVCKVHITAGDYEARKMSRACVSEDEERHGVVLACRVYPRGEISLEVIGKMARAIGT
                     AAPGPGDVSGV"
     gene            32303..33913
                     /locus_tag="PKX01_03045"
     CDS             32303..33913
                     /locus_tag="PKX01_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMZ75127.1"
                     /translation="MNSSAEAAVPSGAAIRPNLDRPSGLVTRYVFLAVMAAGLLFTGY
                     SVFADIAESGAVVTTLLPFVLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPHV
                     AVVWSGVFNFIGVLVSSGAVAFGIISLLPVELILQVGSSAGFAMVFALLIAAILWNLG
                     TWWLGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWAQATKVGYSLLLSPLVGFG
                     CAALLLLALRALVKNRALYEEPRSDAPPPWWIRGILILTCTGVSFAHGSNDGQKGMGL
                     IMLILVGTVPLSYALNRAMPASQVTQFAAVAHVTQDALVKAAPVAPPAEPRKVLSDYI
                     RTHEVTPEVVPALAALTGTIGAQVKDYPSLARVPAESVPNVRNDMYLASEAIRFLEKS
                     DQVNFDADAKANLKAFKGQLDTATKFIPLWVKVAVAVALGLGTMIGWKRIVVTVGEKI
                     GKSHLTYAQGASAELVAMTTIGAADLFGLPVSTTHVLSSGVAGTMAANKSGLQMATIR
                     NLLMAWVLTLPAAILLSGSLYWLFAHLF"
     gene            33934..34869
                     /gene="ppk2"
                     /locus_tag="PKX01_03050"
     CDS             33934..34869
                     /gene="ppk2"
                     /locus_tag="PKX01_03050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HMZ75128.1"
                     /translation="MSTSHPVDQEELLRRLQRDLIDGYDEELEMEIEDRPWTDPMQPI
                     AVDEAEAEAHRQQRHRYFQDLFRLQGELVKLQDWIATTGQRAVILFEGRDAAGKGGVI
                     KRVVQRLNPRVCRTVALPAPSDRERTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVEK
                     VMGFCSDDEYEEFFRSVPEFERMLVRSGIKIVKYWFSITDDEQQLRFLSRIHDPLKQW
                     KLSPMDMESRRRWEDYTRAKEIMLERTHIPEAPWWVVQAVDKKRARLNCIHHLLQQFP
                     YEEIQRPPVLLPARERHEDYQRSAVQSDLFVPEIY"
     gene            34922..35938
                     /locus_tag="PKX01_03055"
     CDS             34922..35938
                     /locus_tag="PKX01_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMZ75129.1"
                     /translation="MSSAALTAYGADPSGLVYGYVFTADGVGRQVDTAAAGAWLAERD
                     ARPGEFIWLHLTGVQAATMNWLQSHLELSEDFTDALKEGSRSTRIELVHASLIAVIND
                     VVYDLSRENTLQVATLWMNVTPRCLVSVRIQPLRSVDRLRAAVKAGETFGSPLALVIH
                     LLREQADMLVHIIRSATEKVDGVEDKFLLGRLPERAALGGIRRDLVRLQRLLAPEPAA
                     LFRLLNRPPAWVSAGDTQDLQQSTEEFSVVLRDMAGLQERIKLLQEEIVAHIGERTNR
                     SVFVLTAVTVIALPINLTAGLFGMNVGGIPLAEDPEGFWRVLLLVLLMTAGAAWYAFR
                     RGPE"
     gene            complement(36014..37696)
                     /locus_tag="PKX01_03060"
     CDS             complement(36014..37696)
                     /locus_tag="PKX01_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMZ75130.1"
                     /translation="MTIKYPENKDLRELIRFSADDGLIWLGESRMLLLQVTTMQALRK
                     ELIHSVGEDQARRVLTRMGFGAGMRDAELARKVRGSRDMLDAFCVGPQLHMLEGSVQV
                     EPIRLDMNPDTGSFYGELLWKNSWEVDAHVREFGPADHPVCWMQIGYASGYSSCFMGR
                     FILFREVECAAAGANTCRIVGKPAEEWPDAAELLRYYDDTAVVGRILELRDEVDTLRS
                     TLGASHQIPSLIGASASFQHAYTLIKKAAPTQVTVLLLGETGVGKERFARALHELGSR
                     REAPFVAVNCAALPHDLIESELFGVEKGAFTGAHASRAGKFERADGGTLFLDEVGEMP
                     LAAQAKLLRVLQDGEVERLGDDRPRKINIRLVAATNVDLQAAVRDGRFRSDLYYRLNV
                     YPVVIPPLRERVSDIPPLAHAMLERFCAVHAKRLKGVTDKALRALKAHKWPGNVRELE
                     NMMERGVILAAHDGWIEAEHLFAGLDISPCSEDRVDASGGLRPATADAAPDVSALCDA
                     VFASGISLDDLEAALLREAMQRQQGNLSGAARLLGLTRPQFSYRLKREKPLA"
     gene            38288..38545
                     /locus_tag="PKX01_03065"
     CDS             38288..38545
                     /locus_tag="PKX01_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HMZ75131.1"
                     /translation="MKSDTPAVDVNRKFVRLVERRANGLVEFEFSIGDPELFVEMLLP
                     ATAFEEFCAVNRVEILEGSRPHEAGSDWDWSLHEATHQRFR"
     gene            38592..39590
                     /locus_tag="PKX01_03070"
     CDS             38592..39590
                     /locus_tag="PKX01_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HMZ75132.1"
                     /translation="MQIDLRTVAINPLRQTYDHIARRIGPDKAASRYQEGTLDLQATH
                     NFHYRPTWDPDHEIFDASRSAIRMADWYAFKDPRQFYYGAYTLARAKQQDTAEANFDF
                     VENRGLAATLPEDLRATVLAVLLPLRHVAWGGNMNGAFIAAYGYGTALTQPSLYHAMD
                     QLGIAQYLTRVGLLLGDDPSVLDDAKTRWMEDGVWQGLRRYVEDTFVLKDPFELFVAQ
                     MVALDGLLYPLVYETIVDDVLSANGGTAVAMLTQFMTEWFAETRKWVDATVKAAAAES
                     PENKALLEKWIGHWRDRAAAALMPVAQIALGSQADAVTSELVSQFNTRLAKAGVSL"
     gene            39602..39871
                     /locus_tag="PKX01_03075"
     CDS             39602..39871
                     /locus_tag="PKX01_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HMZ75133.1"
                     /translation="MSKVFIALQTNDETRQIIDAILADNPHAIAVNQPAMVKIDAEGS
                     LVVRRSTIEELLGRDYDLQELHINLISLTGHVDETDEELTLSWNA"
     gene            39891..41444
                     /locus_tag="PKX01_03080"
     CDS             39891..41444
                     /locus_tag="PKX01_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="HMZ75134.1"
                     /translation="MDMKVSKKKLGLKEKYQHMTRGLGWETSYQQMDDVFPYAKYEGI
                     KIHDWDKWEDPFRLTMDAYWKYQAEKERKLYAIIDAYAQNNGHLSVTDARYINAIKLF
                     LNGISPLEYMAHRGFAHVGRQFPGVGPRVACLMQSLDEIRHAQTQIHALSNYNKYYNG
                     FHEFRHMIERVWYLSVPRSFFDDAVTAGPFEFMVAIGFSFEYVLTNLLFVPFVSGAAY
                     NGDMGTMTFGFSAQSDEARHMTLGLEVIKFILEQDPDNLPIVQRWIDKWTWRGYRVLT
                     LVGMMMDYMLPKRIMSWKEAWEVYFEENGGALFKDLARYGIRPPKWLDQIAFDKDHLS
                     HQAWATFYQYGAVCNFHTWMPTDDEMDWLSKKYPNSFDKYYRPRLEFWRDEAAKGNRF
                     YNKTLPMLCQTCQIPMLFTEPGDPTQICYRQVDYKDNTYHFCSDGCKEIFEHEPEKYI
                     QAWLPAQQIYQGHCFNEGVDPTAEGFDPLGAVLDYYHMAAGRDNFDFDGSEDQKNFEA
                     WRGLATKNA"
     gene            41516..41875
                     /locus_tag="PKX01_03085"
     CDS             41516..41875
                     /locus_tag="PKX01_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HMZ75135.1"
                     /translation="MGVAAIKDYTFEPADRLENFHGNQLLNIGWDQHLLFCAPFCLPL
                     PPTTRFGDIVDKILPGIFGYHPDFAKIDWSTVQWLKGGQPWQPDFARTLAELGLRHKD
                     ALRFRTPGLNGIQGSAS"
     gene            41878..42933
                     /locus_tag="PKX01_03090"
     CDS             41878..42933
                     /locus_tag="PKX01_03090"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HMZ75136.1"
                     /translation="MGYELTIEPLGQTIEVEEGQTILDASLRAGIWLPHACCHGLCAT
                     CKVQVVDGEVDHGEASSFALMDFERTEGKTLACCARLESDVTIEAEIDEEPDAESIPV
                     KDFSGTVSRIETLTPTIKGLFFTLNEPLHFQAGQYVNLEIPTLGISRAFSLANVPSTG
                     REVELNVRIVPGGAGTGYLHERLKVGDPIRLSGPYGRFFVRKSAQVPLIFMAGGSGLS
                     SPRSMILDLLAEGSTLPITLVYGQRRREELYYHDEFVELAARFPNFSYVPALSDEPAD
                     SGWTGHRGFVHEAAKAHFDNDFRGHKAYLCGPPAMIDACISTLMQGRLFENDIYTEKF
                     FSAADAQQVRSPLFRRV"
     gene            42985..>43173
                     /locus_tag="PKX01_03095"
     CDS             42985..>43173
                     /locus_tag="PKX01_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75137.1"
                     /translation="MSRSCIKLATSSAEPTGGHQAPSAEDSQKEEETIMAMTGVLRPG
                     HMQLRVLDLEEGVNHYKNV"
BASE COUNT         6844 a        13518 c        15488 g         7323 t
ORIGIN      
        1 ggcggcgcgg actggttcgg acgccggcgc gacggagggc ggcggcaggc gcagttcgac
       61 ctgcaggggt gcggggcgct cggtgggatt cggcagcagg ccgtagtgcc ccagcagcgc
      121 gagcgcgtgg gcgccgatgg aggcggccag ggcgaggcgc agggtgcgcg gcagggccgg
      181 cgcgggctgc cagggccagt cgtggggcag ggcaggggtc atcatgggcc ggacctccgg
      241 gggcggtgca gggcggcctg ctcataggcg ggatagagct ggccgacgga gcgcctgtac
      301 tcggcttcga ccacggcgag gcggcggaat tcggcgggaa tcggcagggc catgacttcg
      361 ttcatgtcga gcccgtcttc ggcggcgctg cgcagggtgc catccagcca ggcgagccag
      421 gcgcgggtct ggcgaatcgg gccggcgtcg ccggcgacag ggccgtgacc gggcaccagg
      481 acgcggaagc cgctggcctg gaggcggtcg agggcggcgt tccaggcggg caggcgggcg
      541 tggggggtgg tcggactgcg gtcgtggaag accaggtcac cggcgaagag gacgccggtg
      601 ctgcggtcga ggacagccag gtcggcgccg gtgtggccgt ccagggcgat gaattcgagg
      661 cggtggccgc cgatctcctg gatgccggag ggcaggcggc gggcgggggt gacgacttcg
      721 gtgccggcca tccagtcgcc caccaggcgg tacatgttgt cgttgaaggc gccgccttcg
      781 gtgtggatgc cgtcggcggt gccggcgagg gcggccaggg tgtcggccgg gaaggcctgg
      841 ttgccgagga tgtggtccgg gtgatggtgg gtgttgagga ccatacggat gggctggtcg
      901 gtgacccgcg cgatggcctg gcggagctgt tcgccatagc gtcgcgaggg gccgctgtcg
      961 atgaccacga cgccgtcggc ggtgacgatg aaggcggtgt tgacgatgtt gccgccgttg
     1021 gcgagggtga agtcctcgtt gcggccggtg agcacccagg tgtcgtcggc gatgcgccgg
     1081 ggctgcaggg cgtagtcgaa gtcggcggcg ccgaggagga gggtccagcc cagggcgagg
     1141 gccgccgccg gggcgcgcag caggcgcggc acgcgaagcc gcatgggggc cgcgccgctc
     1201 atttgtcgat ctccgcggcg atggtattgc cgttgttgtc ccggccgttg atccggtagc
     1261 cgtggccggt gtgcgcggcg gggatgtcca cggtgaacac ggggttctcg gcgatgggct
     1321 cgaagccgcg cacgcgcatc agcggccgcc cgccgtcgtc ggtgaggcgc aggtcttcga
     1381 tgtagaaggc cgggatgccg gcggcgaggc cggtgtcctc cgggtggacg atgcgcaggc
     1441 ggatgcgctc gccgccttcc ttgcgcgccc acacgcggct gctcacttcg ccgaggcggc
     1501 gctgccattc gggcgagctg ctgccggtgg agggcagggt gcagccgccg ccggtggtgt
     1561 tcacccagct gccgcccacg tgccagagac cgtcggcgcc acgggcggcg gcgcggatgg
     1621 gggtggattg ctggagcttg acgcggaagc cgagggtcgc cagcgccccg ccgggctcga
     1681 agcggacgat ctgcacgatg gggttgtagt cggcaaagac gcgcacttcc tcgacaccgt
     1741 ccagcgcact ggcgtccacg gtgatgggga cgttgagggg gttctcggcg ctgatcgggg
     1801 cgatcacgcg gatgcgctcg tcgaagacgg tgcgggcatc ggccggcagc acttccttgc
     1861 gcatgtcggc ccagcggccg gagttgaggg ggtcgggcgg gtctgccggt gcggcggcca
     1921 gggccgcgtg gcagaccagg gcgaacatca gggcggtgat gatgcgcggc atggtctcgg
     1981 tgtctcctcg tggcggctcg tcggtgcggc acgctctgtc gatccccgga ttctgcaaga
     2041 cctgtgccat gaaatcgtat gggatctcta cagtgtccgt gccggccgaa ggggctggtc
     2101 ggggggtgtg ttcagcgatc cggggctttc cgtggcgggg gtctccggat gttgcagtgc
     2161 gggtgtgtta aggcggtgtg ctgcactttg gagcacttgg tttggaatga agcagcgatg
     2221 gcgcagtgtg tgccggaatg cgcgtggcga cgcgcattcc ggggaacggg ggtcatgtcc
     2281 aagccgcggt tttgtatcgg gaaattttcc cggatgcggg gcgtgtggaa tttcgtcacg
     2341 accaatggca cagctcttgc agaccgccat tgctgcacgg tttggttggg ccgggaggtg
     2401 gccgccccga atagtgcttc agcaccctct caaaggagac actcaatgat ccaaaggagt
     2461 caaaagatga aaaagccgtt tgcaatgacg gtgatcgccg ccgccgtgat ggttgccggc
     2521 gctgcccacg ccaaggaagt gacggacgcc gacattctca acgacgccaa gacccccggc
     2581 gacgtggtcc atttcggtct cggccagcaa ggccagcgct acagcgccct ggaccagatc
     2641 aacaccaaga ccgtgaagga tctggttccg gtgtggtcct tctcctttgg cggtgaaaag
     2701 cagcgcggcc aggaatccca gccggtcgtg ttcgacggca agatgtacgt gaccgcttcc
     2761 tacagccgtc tgtttgccct ggacgccaag accggcaaga agctgtggaa gtacgagcac
     2821 cgtctgcccg agggcatcat gccctgctgt gacgtgatca accgcggcgc tgcgctctac
     2881 gacaacctgg tgatcttcgc caccctcgac gcccagctcg tggccctgaa caaggacacc
     2941 ggcaaggtcg tgtggaagga gaagatcgac gactacgctg ccggctactc tgcctcggcg
     3001 gctccgctga ttgtcaaggg cctggtcatt accggcgtgt ccggtggcga attcggcgtg
     3061 cagggccgca tggaagcccg cgatgccaag accggcaaga tggtgtggat gcgtcccacc
     3121 gtcgaaggtc acatgggtta caagtgggtg aatggcgaga aggtcgagaa cggcatctcc
     3181 ggcaccacca acgccacctg gaccggcgac atgtggaaga ccggcggcgc ggccacctgg
     3241 aacggcgcca cctacgatcc ggagaccaac ctggtcttcg cgggcaccgg caacccggcg
     3301 ccgtggaaca gccacctgcg ccccggcgac aacctgtact cctccgccac cgtggccatc
     3361 gatccggata ccggcaagat cgtgtggcac taccagacca ccccgcacga cggctgggac
     3421 ttcgacggtg tgaacgaatt cgtgtccttc gactacaagg atccgaagtc cggcaaggtc
     3481 atcaaggccg gcggcaaggc tgaccgtaac ggcttcttct tcgtcatcga ccgcaccaac
     3541 ggcaagctgc tgaacgcctt cccgttcgtc aaggacatca cctgggccaa gggcatcgac
     3601 ctggccaccg gccgtccgat ctacaacgac gaaaaccgtc cgggcgaccc gtctgccggc
     3661 accgatggca agaagggctc caccgtattc gcggccccgt ccttcctggg cggcaagaac
     3721 cagatgcaga tggcctacaa cccgaagacc ggcctgttct acgtgccggc caacgagtgg
     3781 ggcatggaca tctggaacga gccggtctcc tacaagcgtg gcgcggccta cctgggcgcg
     3841 ggcttcacca tcaaggccct gcacgacgac tacatcggcg cgctgcgcgc cgtggatccg
     3901 tctgcaggca agatcgtctg ggaaaacaag aacttcgctc ccctgtgggg cggcgtcctg
     3961 accaccggcg gcaacctgac cttctacggt acccccgaag gctacctgaa ggcgctggac
     4021 gccaagaccg gcaaggaagt ctggtccttc cagaccggct ccggcgtggt ggcgcccccc
     4081 gtcacctgga ccgaaggtgg cgagcagttc gttgccgtgg tgtccggctg gggcggtgcg
     4141 gtgcctctgt ggggcggcga cgtggcccgt cgtgtgaact tcctcgaaca gggtggttcc
     4201 gtgtgggttt tcaagctgca caagagcaag taagacgcgc cggatggacg gcccgccgcg
     4261 gcgggccgtt gcaggcggaa aacggcacgg cgctgcggct tccgtgccgt ttttcttgac
     4321 gcagggcaag atggcacatg ccttgcaatt gatttcatca tgaaaatttc gggggttccg
     4381 tcgatggatc tgcgctggaa gctgttcggc accgtgctgg gctttgccct ggccttgctg
     4441 acggcagccg ccgcgtggat cggcggggcc ctgcagcggg acgcgacgca ggaaatggcc
     4501 gcgtcggccc gcctggtgga tgcgatccgc gcggcaagtg ctgcgcccgg catgcgcgtg
     4561 gacgagctgg agcgcctgct gggcgcggag cccctgcgcc acgtccgtct gcagatagag
     4621 cgctccaatc tggaacactc cgtgtcggac gcgcctgcgg ggccgatgga cctgctggcg
     4681 gccgccctgc tgggcgaacg gaccgtgcct gaacagcgca ttcccctggc ggatggcgtg
     4741 ctggtggtgc gcgccgatgc gcacgcggag attcgcgaga tcctgtggga cggcgccggg
     4801 ctgatgctca ccctgctggt cttttccgtg gcgatggcgg ttgttgcgtg gctggccgcc
     4861 catcgggccc tcaaaccggt acgcgagctg gaggcgggac tcgcccgcat tgcccgcgac
     4921 acggggccgg tgcggctgcc ggagttcgaa ctacgcgagt tctcgcgtat cggggtggcc
     4981 atcgagcgcc ttgccggcga tctggcccgc gagcgcgccg accggcagac gctggctcgc
     5041 cgtctggtgg aacttcagga agccgaacgc cgcgagctgg cgcgggaact gcacgacgaa
     5101 gtcggtcagg cgctgactgc gatgggcgtg cgcgcggcct tcatcgaacg ccacgccggc
     5161 cgggcggacg ccgtgcgcct gagcgcctgc gcccgcgaca tccgcaccga atccggacga
     5221 ctggtgaagc tggtgggcac tgtgctggcg cgcctgcgcc cccgcggcct ggacgatggg
     5281 gcgcaggtgg ccgcggctct ggacggcctg gtgcgcacct ggcagcaccg ggcgccggag
     5341 gtcgctattg aggcggacct gccggcgcgt ctgcccgcct tgccgccgtc cgccgggctg
     5401 gcgctctatc ggaccctgca ggaagcactg accaacgcgc tgcgccacag tctcgcagat
     5461 cgtgtgacct tgtcgttgcg ggccgatgcg gacgcgatcg acctggtggt cagtgacaat
     5521 ggcggcggtc gggctacgga tgtcgctgcc cggggccgcg gcggcctgct gggcatgcgc
     5581 gagcgggccg agatggccgg cggccgggtg tggctgtcag atgggccgga cggcggcctg
     5641 tcggtgcgat tgagtcttcc gctccacggt ggcgacaggg tgcctgtgcc tgccgtgtga
     5701 gtccctgttt cagcgagcaa accctttttt accaaggaga aacaccatga acgccttatg
     5761 caaagccgcg ctgctggccg gtctggccgc ggttgccacg accgcgtcgg ccgcagcgga
     5821 tgatgaaatg aaggccctcg ccaccaagag cggctgcctg acctgccacg ccatcgagtc
     5881 gggcacgccc ggaccgaacg gcatggcgcc catcggcccg gcctggcagg acgtggccaa
     5941 gcattacaag ggcaagccgg gtgcggacgc gttcctgacc cgtgtggtgc tggaaggctc
     6001 caacccctac ggcagccact ggaaggacca ggtgagcggt ctggcaatgc cccccaacaa
     6061 ggtggccatc aaggaagccg acgccaagaa gctggtgaag tggatcctga ccctgtcgcc
     6121 gaagtaagcg caggagcggc agggtgaagc cctgccgaac aaggaatgaa agccccggtc
     6181 agcccccctg ccggggcttt ttgctgggcg acggtgatct ccctcatcca ggctggcgcg
     6241 cacggcctgc cgcctactgg gcgctggtga cttcttcaaa gttgccgtcc tgcagaaccc
     6301 catcgccgct gaacacgagc tgcaggatca cgtcggagcg aacgccaaac caggacttgg
     6361 aaaccgggat cttgccgaag atgcacagtc ccccctgggg cggctgcagg gcggcaatcg
     6421 gtgagttgcg caccaggcct tcgcattcca tggtgttgcg gatcgaatag gtcgtgccat
     6481 gggcggtgag cttttcgcgg gcctcggcga tggacaggcc gggacggaac tcctgtcgca
     6541 ggcgttcggg cagatcgctt ctgccggagc aggcatttag ggcgagggcg cacaggggca
     6601 ccagacacag ggtcgccagg gtggggcggc gcggacggga ggtcggacgg atcttcacgg
     6661 gcaggctgga cggcacacgg aggcgcccag tatcgcacga cgtacgcccg gggtcgggtt
     6721 cagtcgtcgc tcgtctggcg cagcttgcgg tagagggtgg tgcgggtgat gcccagctca
     6781 cgggcggcgg ccgagatgtt gccgttgtgg cgctggaccg tatcttccac caggcggact
     6841 tcagcagcgc gcaggctttt ggcggccggc acggggtgga cggtgtcgtc gccgtcaagg
     6901 aggtcctggg gcagatggtc gggacccagg gtttcctcgt cgtcccccag catggcaacc
     6961 ccgaggcgga gggcgttctt gagctgacgg atgttccccg gccacggatg gcggcggatc
     7021 aggtcgaggg tggaggtggc gaggcggatc gggcggtccg gcgattcctc ctgcaggatg
     7081 cgtcgtgcca tgccttccag atccgagcgc tcccgcagcg gcgggagcgc caggctcatg
     7141 gccgacaggc ggaagtacag gtctgaacgg aactcgccta cttcgcacaa ggccttgagg
     7201 gagcggtggc tggcacacac cagcgacagg tcgatctcct cttccgggcc gccgcccagc
     7261 ggggtgacgc gacgggtttc cagaacccgt agcagcacag cctggagttt gaggggcatg
     7321 tcgccgattt cgtcgaggaa gagggtgccg ccgtgggctt cccgcagttt gccgcgggcg
     7381 ccttcgccac gggcgccagt gtaggcgccg gcggtgtaac cgaagagctc tgcctcgatc
     7441 aggttgcccg ggatggcggc gcagttgatg gccacgaagg ggccgtcacg gcgcgggccg
     7501 ctgttgtgga aggcctgggc gaagacttcc ttgccggtgc cggtctcgcc ctggatcagc
     7561 agcgggatgt ccaggcccag aatgcgccgg gcgcggcgga tggccgtacc cacggccttg
     7621 tcacccaggt ccatgtcttc cagcgtcagg cgcggtcgcg ggcgcggcag gacgcgcgga
     7681 tcacgtacgg caccggccgg gcggacgcgg cgcaggttgc tcacgagcac tgtggcggca
     7741 aactcgcggc cacggttgca gcgcagcttg gtgggccgga agctgctgcg cagggcgtgg
     7801 tcgagcagca cgttccagcg ggtctcgaag atgtcgttga aggcggactg gctgtggcgt
     7861 gtgtcgggca agcccagcag gcgttcagcg cgccggttgc aggccagcat gccgccactt
     7921 tcgttgaaga cggccagtcc ctccagcggc gacgcgagcg cttcgggctg atgatggaag
     7981 cgcagcacca ccgacgcgtc gtccagggtc tcgatgaggc ggttctcgat gatctcggtg
     8041 gtggtctgga gcagggccag ggcgtgcacc tgggtgagcc gggcgctgca cgagatgtcc
     8101 aggatgccca gcacgccgcc ggtgggggcg tggatcggtg cggcgatgca ggtgaggaac
     8161 ttgttgcggt ccagaaagtg ttcctcgccg atcacggcga ccgggcgttg ttccgtgagt
     8221 gccgtgccga tggcgttggt gcccatgacg tgctcggacc agcagtggcc aggcttgagg
     8281 gccacccggg cggcccgttc gagaaagtcc gaatcgccca ccgagcgcag gatcacgccg
     8341 gaatcgtcgg ccagcaggat ggtggatgag gagcgcgcga tctgctggtg gaggtgctcc
     8401 atgatgggct gggcgtacgc caccagacgg gcgttggcct ccagccggtc cgacaggcgc
     8461 acgcgcgcgt tggggtcgtc cggtagacgt tcatccgcgc gcaggccccg ggtcgcacag
     8521 cgttcccagg agcgccggat ggtatccacg gggagattga taaggtgctg gtcgatcggg
     8581 ctcacgctcc catgacagca ggaagcatgc ctgtcatgca gggccgcatg tcccccccaa
     8641 acccatcggg caggtgtgct ggagtgactg atttcattca attttatgga acgctgcggc
     8701 ggctttgcgg ccgaggcggg tactggggcg ggtgcacagt gttccggtgg tgagtttctt
     8761 ggcgttgcgg gatggagcag gtgtgcagcg gctgaacagg aattcagcaa ccggacgcga
     8821 ggggctttcg aggcgattcg tcaagcttat gaaacaaaag ggaaatttga tttttgattg
     8881 aacggcgggt ggcatgcacg gtttttgctg aaatcaggag gcaaggcggt ttttcgggcg
     8941 gccatgggcc gccccatttt ttcctgccgg aaagaccgga cttgcccagt caaaccactc
     9001 gaagaaggag gcgtttcatg aaatttgtcc agaccgtctg tgccatcgct ctggggtgtg
     9061 cggtcgtctc acccgcgctc gcatcggaag cgatcatcaa gaaggcccgc tgtgtggcct
     9121 gtcactcggt tgaggaaaag cgggtcggcc cggcctacaa ggacgtggca gccaagtaca
     9181 agggtcagaa ggatgcggag gcagcgttga tcgccaaggt gcgcaatggg ggttccggca
     9241 actgggggca gattccgatg ccgccgcacg gcccggacaa gatcagcgat gccgacctga
     9301 aggcggccat cgagtggatc ctgaagctgt gatccagaac gtccggcggc actctgggcg
     9361 tgcgttcctg gatggtgtgt agcggccggg cgatacacct gctccaatct ggagcaggtg
     9421 tatcaagttg acgctacgtc atgcaatcga ccggcgggaa gtcggggcgc tttcgcctga
     9481 gcttccagcc tcgtttcagc gaatgacgac accccagggc agttcaccga cggggacgtc
     9541 tccgatgttt tcgaggcggg tcgtatcaat gaccgatacg ctgttggact tgccgttggc
     9601 cacgtagagc ttgcgcccgt cgggcgtgag ggccatgttc cagggccgtt tgccgaccgg
     9661 aatcgtggct tcaacctgat tgtcggccgt acggatcgcg ctgaccgtgc cgtccttgcc
     9721 gttggacaca tacacccggc tgccgtcggg cgccacggcg attccgttgg agaaggtgcc
     9781 cgtcttgatg gtggcggttg cctgacgcgt cgtcacgtcg agcgcatata cggtgctgtc
     9841 cagttcgcac gccacatagg cgcggcggct gtcgggcaga aagccgatgc cccgcgggcg
     9901 gcggccgacg gcgatgctcg ccacctgctt gcgcgcggcc acgtcgatca ggtctacctg
     9961 ctccgcttct tcggcgctga ctaggagcca ctggccgtcc ggactgaaca ccgcgtgttc
    10021 cggatttttg ccctggacct tgatgtcggc caggaccttg ccggtggcgg cctggatcag
    10081 ggagacgctg ttggattcct cgaccgccgc ggcgatcaga cgaccgtccg tcgacacgct
    10141 gaccccttcg ggcgattcac ccaggcggat gctgcgcaga agttgacgcc gtgccagatc
    10201 gaccaccacc aggctcgatg aaggcgcctc gctgatgtag agccgctgcc catctgtggc
    10261 gatgccgcgt gggcgtttgc cgccgggcag ggtatccacc acgctgtcgg tggccgtgtc
    10321 gatcaccgag acggtggccg acttttcgtt ggggacgtag gcaaaaggcg cggccagcac
    10381 gagcccggga aaggccgtgc aggccagcag gagccggccg gaaagcgtga ggcgcatggg
    10441 tgtgtcagac ggtgggtttg aggtaggtca ccccgtgacg ggcaaagatc tccgccaccg
    10501 taccgtcctg ttcgagtgcc ttgagggcgc cggccagggc gtcggcgagg ggggcttcat
    10561 cggccttgac ggccatgccc aggggccagt tgttgatctt gagttccggc atctggacga
    10621 tgtccacgat gaaacgggtg ccctttccga gggccacttc gatctcgctg cgtggcccca
    10681 tgacagcgat gacttcgccg gccttgagct tctcgatggc ttcggcgatg tccttgcaat
    10741 gcacgacgtt gtcccgcagg cggccattga gcacgcccag caggaaggca tcggacaggc
    10801 tgcggccttc gacggcgatc ttctcgcggg tgaagagttc aagcgcattg gcggcggagc
    10861 cgcggaaggc gccgacccgc gccggatccc gcgccacggc gatggtctcc aggtgatagg
    10921 gcccgaagat gcggaccttg tcgttcttgc cctggaggta ggaatccacc ggcacgtgca
    10981 tcatcacgtc ggaggggcgg gcgcccaggt aatggccttt ccagaccatg ttgcgcaggt
    11041 cgtcgtccat gtcctcatcg gcgttgtaac cgatgacctg cgtggcaaga ccgagcttgc
    11101 gtccgagggc ttcgccgagc tccacgtcga tgcccttgcc cttgctggag aagggcgcga
    11161 aatcgttata caccgccacg gtcagggaac cgcggcggcg gatgtcctcc agcccgtctg
    11221 cacgggccgg caggccggcg gccagcaggc aggccccggc cgcggtgagg aattgacgac
    11281 gatcagtcgg cacgaacggt ctccagccag gcgcggatgg tccacatggc ttcctgggac
    11341 aggatacctt caaacggggg catataaacg cgaccgtcgc gggtggcgcc atggcggatg
    11401 cggttcagga agatctcgtc gccttcctgg ccttccggca ggtagcgcag atccggtgcg
    11461 atgccgccgg agacggcgcc caggccatgg cagcgggcgc agttctggtt gaaggcggag
    11521 gagccgatgc ggatggcttc cttgtccttg ctgtacgggt ttttggcgag ccattccttg
    11581 ccgaccggct tgagggtgga cgtatcgacg gcctggggcg tcacgtcacc gtgggcgaag
    11641 agggcgccgg cagcgaggga gagggcagcg gcggtggcaa gacggaaaag ggaggattta
    11701 cggacggtca cctggatcac ctttggttgt gtagttggag tcggtcgggc ggatcgccgg
    11761 ggcgttccgc gtctgcgttc ctcaacagca aggggcgtgc cagtcgcctc cggggaatgt
    11821 ttgatggagg gggatctgga aaggcgagga tttttcagtt tttctgatgg tgcaataaac
    11881 tggcccaaca cttgctcagg tggatgcact gtggagcagt cacgttgctg taatgtggtt
    11941 cagctgctac attttggagc atctcggtgc agggatcgga aagtgtggcc agcgcccatg
    12001 gcttaaccca ccttcctctc gtgccgatac ataagccggc ggcggtcatc cgtcagtcgg
    12061 cgccgacaat ccgtattgaa cggaggagga gatgattcag gttccttata gtgtggaggc
    12121 gcgctcggtc agcctccagg aggcgcggtc actctacttc ggtcggggcg aactccctga
    12181 agccctggtc gatcagagtg tgctgcgttc ctgggaacga tgccggcagg ccgggctcaa
    12241 ccccgcacgc ctcgaatgcg gcgacccgaa ccagcggggc gtcgtggaag cgcgggagcg
    12301 caaccgtacc ttgatgtccc atgcccagcc ggtgctcgaa cacctgttcg agcagatccg
    12361 caattcccac agcatggcca tcctggccga cgcccggggc atggtgctgc agacctacgg
    12421 cgacgcggat ttcctgagcc gtgccgaaca ggtggccctc agtcccggtg ccagctggca
    12481 cgagttcgaa cgcggcacca acgccatcgg aacagccctg gccgaacggg tgccggtgaa
    12541 cgtgttcggg tcggagcact tcttcgaagg caacgccttc ctgacctgca gtgcctcgcc
    12601 gatcctcgat ccgcacgggc gcctgatggg tgtgctggat atctccagcg actaccgtgc
    12661 cttccagcgt cattcccttg gtctggtgaa gatgtcctcc cgcattatcg aaaaacgcct
    12721 gttcgaagcc gaattcgccc atcagggcct gctgagtttc cattcccgtc ccgatcacgt
    12781 aggcagcctc tgtgaggccc tgctggccat cacccccgac ggcgacctgc tgggtgccga
    12841 ccaggtcgcc ctcgagctcc tcggcatgcg ccgggaggat ctgccgcgcc ggcattacgg
    12901 catgctcttc gacgtgcccc tgggtacgct ggtggaccgg gcgcggcgcg accccctgtc
    12961 gctgattgcc ctctccggcc gtaatggcga gcgcttctat gcccgtctgc ggggtaactt
    13021 tgcggcaatg ccggtggcgt ccacgggcct caccgatgtc cgcggcaagg cgctcaaggc
    13081 cgcggtcgag gcgcctgctg cgccgacccc cgcgccggtt tcggtcagcg cgggtgcgtc
    13141 catggacaag agccggctca cgctgcaatc cctggcgacc ggcgacgaac gcctctaggc
    13201 ggccatcgac aaggggctgc gggtgatcgg ccgggacatc cccatcctga ttcaggggga
    13261 gtccggtgct ggtaaggaga tgttcgccaa ggccttccac aacagcggac ctcgtgccca
    13321 ggggcccttc gtggcgctca actgcgcggc gatcccggag accctgatcg aatccgagct
    13381 cttcggctat tcgggcggtg ccttcaccgg cgcacgcaag gaaggcgctg tcggcaagat
    13441 ccagcaggct catggcggta ccctgttcct cgatgaaatc ggcgacatgc cgctgcccct
    13501 gcaggcgcgt ctgctgcgcg tactgcagga acgctgtgtg acgccgctgg gcaccaacaa
    13561 gagcatcccg gtggacatca ccctggtctg cgcgacgcac cgcaggctca aggaagaagt
    13621 ggcgcggggc ggattccgtg aggatctgta ctaccggctc aacggactct gtgtgaatct
    13681 gccgtccctg cgggagcgca gcgacatcga ccgtctgatc gacaagctgc tgcgttgcga
    13741 agcgggcccg aaccgctcgg tacgggtgtc ggccgaagtc atggatgcct ttcgtcgcta
    13801 caactggccg ggcaacatcc gtcagctcaa caatgtcatc cgcgtggcgc tggcgctgct
    13861 ggacgacgac gaggacgtca tccgcccctg ccatctgccc gacgacatct ttgaggagat
    13921 cggtcagctg gctgcggctg cccccgctgc gccgatcaag gtgccggggc tggtcagcga
    13981 agtccggccg atggcggtgg ccgagcccgt tggcgcagcc ggtatcggcc gcctggaaga
    14041 gatcgaacgg gaaatgattc agcgcacgct ggaggcggaa aagggcaaca tttctgccgc
    14101 ggcgcgtcga ctcggcatca gtcgcaatac gctgtatcgc aagctgggac gtttttgagc
    14161 ggctcggccg tttgacgcga ggcgcctgcg ggcgcctctt tttttttgcc ggtaagccac
    14221 acccggcggt ggccggccgg cgccgtccgc acaagttcat tctcaccccc cgtcgggcct
    14281 gctaggcttg caaaggacgg gatggcccgc cgctgcgcgt cgacgcggcg ctgaaggaga
    14341 acaacaagat gtggcagaag gtgcactgga acgggccctg ggcccgggga gcgaaatggg
    14401 gcggcggggt gctggccgtc gtcggggttg tgggctttct ggtggcgcca ccggtggtgc
    14461 gctcggtggc ggagtcggcc ctcacccagg ccttgcaccg accggtgagc atcgaggggg
    14521 tgtcgatcaa tccgtatgcc ttgtccgctg aggtgcgcgg cctgcgcatc gatgagcccg
    14581 gcggtggcgt cgaggcggcc agtttcaagg cgctttatgc aaaccttgag gccgagtccc
    14641 tgttccgtgg cggcgtggtg gtaaaggaag tccagctgat cgagccccac gtgagcgtga
    14701 tccgtacgga ggggcagcgc tacaactggt cggacctgat cgatgagatc ctcaacaagc
    14761 cggacgatgg atccaagacc ttcttctccg tccataacat tcgggtcgag catggccgtg
    14821 tggattttga cgaccgtccc acggggcaga agcacacgct ggccgacctg aacctgggcg
    14881 taccctttgt ctccaatctg ccgtcccagg tggaaaccta tgtcgagccg ctgctcgccg
    14941 tgaccctgga tggcgcgccc ttcgaggtcc ggggcaaggc ccggccgttt tccaaagccc
    15001 gtgaatcggc ggtcgagctc aggtttgaag gcgtcgacct cacgccgtat ctggcctatg
    15061 tgcccctcga gaagcccttc cgtgtgccgt ccgcgcggct gtcgtcgaat ctcgtggtga
    15121 ccttcgccca accggacggc caggcgccgg acgtggcggt gaagggtgag gtggggctcg
    15181 atgctttcga actgcaggac gcagcgggac atccggcggt caagctgcct cacgtcgcgg
    15241 tgggcatcga gcgtcttgcc cccctggccg gtgtcgtgtc tctgggaaca gtgaccgtcg
    15301 aacgcccgga agtcgtcgtc gatcgggacc gggacggccg gctcggtctg ctgaagctcg
    15361 tcccgaaacc cgccccgtcg tcagagcccc cgtcgccggc ggctgccccg tcggacactc
    15421 cgccggcgag gccgcttgcc gtcacgctcg gcgaattcag actgaccggc ggcaaggtga
    15481 tgatttccga cgcgctgccg ggcggaacct accggcgcac gctggacgat ctgcatgtca
    15541 gcctgcgcaa gttcgatctg gcgggagccg agcccgctgc actggacgcc ggcttccaca
    15601 ccgatctggg cgaatcggtc gagcatgtcg gcaccgtggg cctggcaccg cttcgcgccg
    15661 agggcacgat cacggtggat cgactcgacc tgccgggcca tgccgcgtat tacgcggcgg
    15721 ccctgaagca ggccgatgtc gtgtcaggtc atttcgacgg caagctggct tatgcgttct
    15781 ccattgagaa ggaagcgccc cgcgtaagcc tgaagggcga agtcctgggc ctgaaggacc
    15841 tggtggtccg cctgaagggg gagaaggcgc ccctcgcgaa ggtggggacg ctggaactgc
    15901 gcgaactggc cgtggatacg gcggcccgca aggtggatgt gggcgaagtg atcggcaagg
    15961 cgacgcggct ggccctgatc cgcgacgggg agggacggtt caacgccgaa cggatgacgt
    16021 ccaccgaggc ggtgccaccg cctgccccgg agaagccggt cagggctgca gcgtccaagg
    16081 ccccgaaagc cgtggccgcc ggtccggcat ggcaggtggc ggtcaggcgt gtgactctgg
    16141 atgactgggg cgtgcgcatc gaggaccaga ccctgtcccc ggccatcgtc ttcaatgccg
    16201 agcccctttc cctgcgtgtc gaagggctgt cgaccgccaa gggcagcaag gcaaaggtgg
    16261 agctgaaggc ctccatcaac aagcgcgggc gcttcggcgt ggcaggcacg gtggggctgg
    16321 cgccgctggc tgcggccctg gacctggatc tgtcggcggt ggacctggcg atgctccaac
    16381 cctacgtgac ggagaaggtg aatatcgcca tcacccgcgg tgtggtgacg tcgaagggac
    16441 gtctcgcctt cgagctgccc gatgggggcg ccatgaaggg agggttcaag ggcagcctga
    16501 ccatcgggga tttcgcttcg gtggacaagc tcaatgccgc cgatttcctc aagtggcgct
    16561 cgctctactt cggcggcgtg gatgtccgtc ttgcgcccct ggccgtcaat gtcgacgaga
    16621 tcgcgctgac caatttctac acccgtctga tcctggacga acaaggtggc ctgaacatcc
    16681 gcgaaatcac cgccgagcgg gcgcgggatc agaagcgggc aaaggacgcc gcccgcgccg
    16741 ccggaaaggg gagcaatgcg gggagcagtg cgccgcccgc gccggtcgct gcaaaggccg
    16801 tggcggcgcc cgctgcaccc actccgccgc ctccggtcac gatcaggcgg atcaccctgc
    16861 agggcggcaa tatcgcctac agcgaccgct tcgtgaaacc gaactatgac gccaacctga
    16921 cggggatggg cggcgtgctg tccgggctgt cgtccgaccc gagcaccctg gccgaactgg
    16981 acctgcgggg ccgggtggac aacgcggcgc cggtcgaagt gtcgggccgg ctgaatccct
    17041 tccgtcagga caaggccctc gatatccgtg cgtcggtgac ggattttgag ctgtccggcg
    17101 tgtccaccta cgcggccaaa tacgtgggct atggcatcga aaaaggcaag ctgtcggcca
    17161 agctgaacta caaggtggaa gaccgcaagc tcacggccag caaccaggtc ttcctcgatc
    17221 aactgacctt cggtgaccag gtggacagcc cggatgcgct caagctgccg gtgcagctgg
    17281 ccgtgtccct gctcaagaac agccggggcg agatcgacct ggatctgccc atcagcggca
    17341 cgctggacga tccccagttc agtgtcggcg gcatcatcgt caaggtcatc gtcaatctca
    17401 tcggcaaggc cttgacctcc ccctttgcct tgctgggctc gctgtttggc gggaacgctg
    17461 aagaactcgc gtggctcgag ttcgatcccg gttttgcgcg aatcgacgaa aaaggagagt
    17521 cgaagttgaa agcgatcgcc aaggccatgt ccgaccggcc cggcatcaag ctcgagattt
    17581 ccggacgtgt cgatccggat gccgaccggg acggcctgcg ccgcgcagcg atgctgggca
    17641 gggtcgaggc actcaaggtc aaggagatgg ccaggaaggg cgaggcggcg ggtgaggatg
    17701 ggcgcgtgcg cgttgacccc gctgaatacc cggtcctgct ggcgcgggtc tataaagacg
    17761 cggatttcgt caagccgcgg accatgctgg ggctcaccaa ggacctgccg gtgcccgaga
    17821 tggaaaagct gctcatcgcc aacgccaagg tcaccgacga ggatctgctc accctggccc
    17881 atcagcgggc tcaggtcgtg aagagctggc tgatcgaaca gggcaaggtg gccggcgaac
    17941 gtgtcttcat tcttgctccg cgggtcggca agggcggcaa ggacgtcagc gccaccctga
    18001 gccgtgtcga tttctccatg cgctgaacct ttgggaggct gtccgatgaa ccggaaccga
    18061 ctggatgccg tacagatcga cgccgccctg gcggaactgc cgggatggca gcgtgtcgag
    18121 ggccgcgacg cactcggcaa gacctaccgc tttgcggact tcaacgcggc cttcggctgg
    18181 atggcacggg tggcgctgat ggccgagagg cttgaccatc atcccgagtg ggcgaacgtc
    18241 tacagccggg tggatgtggt gcttgcgacc cacgacgcgg gcggcgtcac ggcgttcgat
    18301 gtggccctgg ctcgcttcat ggagtctgct gtgtaggtgt gcacgccttt tcgcccgaat
    18361 gcggcgctgc gacatgcttc gcggcgccgg tggtggtacg ctacgccccg atccgaccac
    18421 gcctccgcgc acgacgcgga acacatctgt ccgggttcct cagcccttcc ccacgggaag
    18481 gcgcaggacg ccccgagaca atgatcaaga acaatgtgga tccccggggg agctgccatg
    18541 catctgaatc tcagtttcga cctgagcgct gcggtccgcc gtgtccgccg ccctgcgtcg
    18601 gtggcggacg cgtccgcgat cggggcaaag gtctggaagt cctttcctta cggcaatcgc
    18661 ctgtaccgct atgacggcgc ggcgctccag gcgaactggg cgcgtctgca tcggggtgat
    18721 cgcgagccgt ttcccagcgc aacacgaatc gaacgcctgg tccgccacaa ccccgcattg
    18781 gctgagcatg tgcgcgaccc ggcgcggacg gctgcatgtc tcgagcaggc ctggcgggcc
    18841 tatcacgcgg gggatttcgc cgagtcggtg cacttcgcgc aggacgccgg agtggccggt
    18901 accatggtgg cggtgcgggc ggcagcggtc tacgcagccc atgtggaaga ccaggacgac
    18961 cggcgtctga aaatcctgcg ggaggctacg gaggcggccg aggaactggt ccaggtggcg
    19021 ccggtgtggg ccaacgcgtg gtatgcgttc gggctggcgg tggggcgcta cagccagaac
    19081 ctctccgtga tgaaggctct caccctgggg ttcggtggca aggtgcgcga cagtttcctg
    19141 cgggcgctgg cactcgagcc ggaccatgcc gacgctcatc tggcgctggg cgtttatcac
    19201 gcagaggtgg tggacaaggt cggatccgtg gtgggggccc tgtcccacca tgcccggcgt
    19261 gacgccgtcg aacaccactt cgagctggcc cgccggctca cgccggactc acccattgtg
    19321 ctagcggaat acgccaaggc gcgcatgctg gccttcgggg ccgcccagtt cgggcgtgcc
    19381 tatggcctgt ttcaggaggc ggcggcctgt cggcccgtgg acgccctgga gcggcttgat
    19441 gtggaactgg ccaagcgcga gttgatttga tgccggtccc gcgcccggcg ggtgaggggc
    19501 ggtccctcag cccttggcgc ggatcaggcg ggccttctcg cgctcccagt cgcgttcctt
    19561 ttcggcttcc cgcttgtcga actgcttctt gcccttggcg agcccgatca atgccttgat
    19621 gcggcccttg gtgtagtgca ggtcaagggg caccagggca tagccagcgc gttccacctt
    19681 gccgatcagc ttgtcgatct cggtggcatg gagcagcagc ttgcgggtcc gggtcgggtc
    19741 cggatggata tgggtggacg cggattcgag ggccgtgatg tgcatgccga cgatgaagat
    19801 ctcgccgttg cggatcacca catagctttc cttgatgttc gaccgtccgg cgcggatggc
    19861 tttcacctcc cagccctcga ggaccagtcc ggcttcgtac ttgtcctcga tgaaatagtc
    19921 gtggtacgcc ttgcgattgt cgatgatgct cattggcggc ggcgcgaacg gatgcgtaaa
    19981 atcgcgtatt ttaccagttc gagcgcaagg ctcgcgaccg atggccgctg tcaagaaaat
    20041 cgtcctgatc gaattcaccc ctgcgcagat gttcgatctg gtggatcgct gcgaggacta
    20101 tcccaggttt ctgccctggt gtggcggaag cgaggtgcat caccgggacg aacggatcac
    20161 ccgcgccacg ctccacatca actaccacgg catcaaatcc catttcagca ccgagaatga
    20221 caagcagtac cccacccaca tgcacatccg tctgaccgag gggccgttca cgcatctgga
    20281 tggcagctgg gatttcacgc cgctgggcga tgctgcgtgc aaggtggagt tcaacctgcg
    20341 ctatgaattt tccagccggc tcatcgaaaa ggcggtgggt ccggttttca atcacattgc
    20401 caacaccttc gtcgattcct tcgtgaagcg tgccggtcag atttacggtg gaaagtagga
    20461 tgtccgattc gatcaacgtg gaagtctgct acgccctgca ggatcggcag gagattgtca
    20521 gcctcaggct gccggcgggc agcaccgtgc agaaggctgt cgaggcgtcg ggcctgctcc
    20581 agcgccatcc cgagatcgat ctggtcaagg ccaacaagct gggcgtgttc gccaagctgg
    20641 tcaaagggga cacggtgttg cgcgacaagg atcgcgtgga gatctaccga cccctcatcg
    20701 ccgacccgaa ggaggtgcgc cgcaagcgcg ccgaagaggg gaaggtgatg aagaaggggg
    20761 gcggctccgc cgacgaggcg gcggcctgag catcgaacac gctcggaaag cccttgcccc
    20821 ggagtgggag tgggtgcgtc tgaggtggga aggctcgtta cgcctgcccc gtgtgacgat
    20881 caggcacggt gcacgacgac gtgcatcgca ccgttcgggc atggtggatg agatgtcctg
    20941 gtcgggtgcg ccacgaagga ttcggacggg cctgagggcc cgtcacttcg ggcgcgtgat
    21001 gttgccatct tggccgtacg tgaccgctgg gtccgcccgt caggtgaatg ctgaagggac
    21061 gaccaacctg caccttcgct gctattgggg cttgcagttg ttttcaacca cacgccgggc
    21121 gcgttcaatt tcggcatcgc gctgggcgtc gtcaagatat tccttttcgc ccgctgcatt
    21181 gaagcgggcg atccgttgcc cgctctccag cccggcgagc tgagattttg ccgctgcgca
    21241 ctcacgctgg cgatcctcgt cgcgggcctt gtccttgcgc gccttgtcct cggcttcggc
    21301 ggcttcggcg cggcgcttgc ggaaggcgag ttccttgtcg gcaaagcttt gtgtcgcgcc
    21361 gtcctgcgca gtcgggggcg gcgcgagcgg ggcctgcttc aggcggcgcg acgtgctgtc
    21421 ggcgggcggc gtatccgagt agtgcgttat gcctgcagca tccttccagc tgtagatgct
    21481 ctgggccagg acaagcgggc tggcaccgag gatcaggagg gcgaggaggg cgcgggacat
    21541 gaacgggact ccggaaggac aaagcttttg cggggcaagg ggcgactgcg tataatacgg
    21601 atttggtcga agggattata gccatgcgag tgatccagaa ggcgctgacg ttcgatgacg
    21661 tccttctcat ccccgcccat tccagtgttc ttccgcgcga tgtcagcctc cgcacccgcc
    21721 tgactcgcaa tgtctgtctg aatattcccc tggcgtctgc cgccatggac accgtcaccg
    21781 aagcgcgtct ggccattgcc ctggcgcagg aaggtggaat cggcatcgtc cacaagaatc
    21841 tcactccgca gcagcaggcg gccgaggtgg ccaaggtcaa gcgcttcgag tcgggcatcc
    21901 tcaaggatcc catcatcatt ccgccgacca tgacggtccg cgaagtgttg accctgaccc
    21961 gcacgcacaa gttctcgggt ctgcccgtgg tggacaacgg caaggtcatc ggcatcgtca
    22021 ccaatcggga tacccgcttc gagacgaacc tggatcagcc ggtctccgcc atcatgacgc
    22081 cgcaggcgcg gctggtcacg gtgcgcgaag gcgccagcct ggacgaagcc cgcgcgctga
    22141 tgcacaagca ccgcctggag cgggtgctgg tgctcaatga ggccggcggc ctggcggggc
    22201 tgatcaccgt caaggacatg atgaagtcca ccgagcatcc cctcgccgcc aaggacgacc
    22261 tgggtcggct gcgggtcgga gcggccatcg gcgtgggggc cggtactgaa gagcgggccg
    22321 cgctgctggc cgaggccggc gtggacgtga tcgtcgtgga tacggcccac ggccacgccc
    22381 agggggtgct ggaccgggtg aactgggtca agaagaattt ccccaaggtc gaggtcatcg
    22441 gcggcaacat tgccacggct gccgcggcgc tggcgctggt cgaggccggc gccgacggcg
    22501 tcaaggtggg tatcggcccc ggctccatct gcacgacgcg gatcgtggcc ggcgtgggcg
    22561 tgccgcagat caccgcggtg gacaacgtgg cccgcgccct cgaaggctcc ggtgtgcccc
    22621 tcatcgctga cggtggcatc cgcttctccg gcgacatctc caaggccatt gcggctggcg
    22681 ccaacgtggt gatgctgggt gggctgttcg ccggcaccga ggaagcgccg ggcgaaacgg
    22741 tgctgttcca ggggcgcagc tacaagtcct accgcggcat gggctccctg ggggcgatgc
    22801 agcagggcgc cgccgaccgg tacttccagg agaacgacgg caacgccgac aagctggtgc
    22861 ccgagggtat tgaaggccgc gtgccctaca agggcgcggt gacggcggtg atccaccagc
    22921 tgatgggcgg cctgcgggcg tccatgggct acgtggggtg tgcgaccgtg gatgagatgc
    22981 gcacccgtgc cgaattcgtc gagatcacct cggcgggcat gcgcgaatcc cacgtccacg
    23041 acgtgcagat caccaaggaa gcgccaaact accacgtgga ttgagggctg ccgcgcaggc
    23101 ggaacgcccc gcatgacacg gaagggcagc ctgcgggctg cccttttcca ttgacgcgcg
    23161 catcgtggag cgctgcgggt gcagattttc cggtacatgc agggacggtg tcggcgcgga
    23221 tggtgaatgt cgtgccgatg attccgcgct gcgggcatgg cgcacccgtg ccccttcaac
    23281 gccgggcgac acgcccacgg tacgacggcg cctggccagg ctgtggccgc gctcatgatg
    23341 gcggtgcgct gtccgggcgg gcgcgacctg tgggcgccgg gagacgcacg ggaggctggc
    23401 ggtcgcgggt ccgatcacgg atggcctgcg cgagcgccgg gcgggcgctg caacgcgtcc
    23461 cgggacgaca ccggcgcgtc atcgtcgcgg tgcgataatc cgactctttg tgcggccgga
    23521 ctgaacctcc ggcctcctcc ggcactccat cccgccatgc tgacccgaac tgttcccctt
    23581 tgcgcctctt ccgcgtccgt ggtcgtggag gtcgccgccc tcagccgccg cgtcgtgtcg
    23641 gagggcgctg cgctgacgat tctggacgac gtgtcgtttt ccatcgccgc tggcgaggcg
    23701 gtggccatcg tcggggcgtc cggctccggc aagtccaccc tgctgggtct gctggcggga
    23761 ttggacgtgc cggatagcgg ccatgtgcgg gtgcgcggac aggacctgtt cgccctggat
    23821 gaggatggcc gcgcggcgct gcgtggggcc agcatcggct tcgtgttcca gtctttccag
    23881 ctcctgccgg cgctgacggc gctggagaac gtgatgctgc ccctggaact ggccggcctg
    23941 cccgatgcgc gggccgcggc gaagggctgg ctggaacggg tcgggctggg cggacggctc
    24001 ggtcactatc cgcgccagct gtccggcggt gagcagcagc gcgtcgccgt ggcgcgggcc
    24061 ttcgcaccgc agccggcact ggtgctggcg gacgagccga cgggcaacct ggatgcggcg
    24121 accggagagg cggtcatcga gctgatgttc gcgctgaacc gggaggcggg cacgaccctg
    24181 gtgctggtga cccacgacac ggcgctggcg gcccgctgcg gacgggtgct gcgtctggcg
    24241 gccgggcggc tggtgtcgtg acgccggccg tcggccgggt cagaacaggc tttccttgag
    24301 cccgcaggcg ttcaggatgg cgcgcccggc ggcgacgccg ctgcgcacgg cggattccag
    24361 ggtcgccggg tagtccgagg ccacgtagtc accggccagc cacaggccgg gttctggcgt
    24421 gcgcggggtg ggacgctcca gacccggtgt gcacgcgaag gtggcgcgct tttcggtgat
    24481 caggcgggac caggcgaggc gcggcagagg gccgagggcg gcggccagct ccgccaccag
    24541 cgcgtcgcgc aaggcgtccc ggtcgagctg ctggtgcagg ccgctggcgc tgatgacgat
    24601 gctgaccagc cccgtcgggc cgcccagggc ggtacggtcg aaggcccatt ggccatagcc
    24661 gccgctgagg ccgatcatgg gttcggggaa actcaccggg ccgtcaaagc ccagccatgc
    24721 ggtcacgatg ggctcgtggc gcaggcgctc gatctgcgtg accaccggcc gcataccggg
    24781 cagaccggtc accagcgcgg ccacgtggta gggcgcggtg gcgatcacca cgtggtcgta
    24841 gcaggtgggg aaggggtcgc cgtcgaggcg gaagcccgta tcgtcccgct ggatggcctt
    24901 gaccggctcg acaatgcgca ccgtatggcc gcgcagggcc agccagcggg aagcgggcac
    24961 cgggaacagt tcggagaggt ccacgcgggg cagcagcagg tccgacgcgg atgcgctgcc
    25021 ggcgaggctg tcccgcagca cgtgggcaaa gacctgggcg gaggcatccg ctaccggcgt
    25081 gttgagggcc gccacgcaga ggggctccca gaccaggcgg acgaggcgtt cggtctgggc
    25141 ggtttcggtg agcagggtgc cgaccgagcg gtctggcgcc acgcggaagc gctgtcgctt
    25201 gaggtgacgc atcaggcgca gcatggcgag cttgtccgcg gtgccgaggc cacgggcgcg
    25261 gagcaggccg agcgccaggt ggagcggtgc gggcagggcc gcggcgcgca gttcgcagtg
    25321 gccgggatac accagggcga ggcggcgggt ggcgagcacg gacggtcgca cgcccaccag
    25381 gcgcagcagg cgcagggttt cggaataggc gccgaggagg atgtgctggc cgttgtctac
    25441 gcgctggccg tcagtctcca cgacccgtgc ccgcccgccg agcacgcggg aggcttcgaa
    25501 gagggtgaca cgggcgccgc cacgggccag ggtgacggcg gcggcgaatc ccgcgtaacc
    25561 gccgccgaca atggcgaccg acggcgtcat ccgcgcagcc aggtgcgccc ggcgatccag
    25621 atcttgcgca ggggcgtgag ggaggtgcgg cgatcgagta cctggaagcc gtcggcctcg
    25681 atctcgtcca gcaggctgcg gtagatggct gccatgatga gtccgggacg ctgggccttg
    25741 cggtcggccg ccgggagctg ggcaaaggcc tgctcgtaga ggccgcgggc gcgctgggcc
    25801 tggaaggcca tcaggctgcg gaaattttcg ctgtagcgcg cttcgaggat ctcgtgggcc
    25861 gggacgttga agcgctgcag ctcgtcgacg ggcaggtaga tgcggccgcg gcgggcgtcc
    25921 tcacccacgt cgcggatgat gttggtgagc tggaaggcga ggcccaggtc gtgggcgtac
    25981 ttgagggtct ggcgttcggt gtagccgaag atttcggcgg acagcaggcc caccacgctg
    26041 gcgacccggt agcagtagag gtgcagggcc ttccagtcca ggtagcgggt ctggaccaga
    26101 tccatctgca tgccgtcgat gatctccagc aactgctcgc ggggcaggtt gaagtgcgac
    26161 gcgatgtcct tgagcgccag gccgaccggg tgatgggggg cgccgtcgaa gacgcggtgc
    26221 acttcttcgc gccaccagtc gagcttggcc tgggccacct gggggtcgtt gcattcgtcc
    26281 acgacgtcgt ccacctcgcg gcagaaggcg tacagggccg tgatggcgcg ccggcgctcg
    26341 gggggcagga agaggaagct gtagtagaag ctggagccgc tggcggccgc cttgtcctgg
    26401 cagtactggt ggaaatcggt catgggaatc aacggaatgc ggcgcgccac gcaaggcgca
    26461 gccagtcggt tctgcccacg gtggggcgtt gccggaagac atcgtagcgg acgtcctcga
    26521 tcttcttgag gatggccagc ccgccctgga tcaccaggcg cagttcccag ccgatgcggc
    26581 cgggcagggc acgggcgagg ggggcgccgt ccgtcatcat gcggcgggcg cgggtgacct
    26641 ggaaatccag cagcgaggcc accgcggggg tgacgacgcc gcgggccaga tcttcgtcgc
    26701 acacgccaaa caggctcatc tcgtcctggg gcaggtagac gcggcccttc ttgagatcga
    26761 cggccacgtc ctgccagaag ttgatgagct gcaggctggt gcagatccag tcggagcggc
    26821 gcaggttctc gggtgtgtcg gcacggtaca ggcacaggag caggcggccg accgggttgg
    26881 cggagcggcg gcagtagtcg gccagctcgg cgaaatcggc gtagcgggtc ttgcccacgt
    26941 cctggctgaa ggcgtcgagg aggtcgcgga agaggccgac gggcaggccg aactggcgga
    27001 tggcgcgggc caggcggtcg aacatcggtg ccaggccggg atcgtcgtag ggtgtgccct
    27061 gctcgatggc atgcagggcg cgacggtagt cgttgaggcg cgccaggcgc atcaccggca
    27121 gggcgtcgcc ttcatcggcg atgtcgtcgg cgctgcgcgc gaaggcgtag atggcctcca
    27181 ccggctcgcg caggcgggcc ggcagcagga aggaggcgac gggaaaattc tcgtagtggt
    27241 cgacgggcat gggcgcggcg gagtataccc tgtgcaacgc ttggctccgc gcccgcgcat
    27301 ccattgcctt tccgtcttcc ggaaccggcc gggcaagccc gccgtccatg tcagcatgct
    27361 cgagatcgat cacctttgcc gcagcttcca ggggcgcagc gtgctgcggg atgtgtgcct
    27421 gcggcttcag cccggcgact acgtggccat cgtcggggcg tccggcgtcg gcaagtccac
    27481 cctgctcaat ctcattgccg ggctggatcg tccggacggg gggcgcctgt ccctggacgg
    27541 cgtggcttac gatgcgcttg acgaggatgc cctgacacgc ctgcggcggg cgcgtttcgg
    27601 ctttgtcttc caggccttcc acatcctgcc ccacctcacc ctgcgccaga acgtggcgct
    27661 gcccctgtgg ttgcaggggc gcggcggggc cgaagcggat gcggcggcgt gggcactgct
    27721 cgaggaggtg ggtctggggg cgcgggcgga cggctggcca cgggaactgt ccggcggtga
    27781 gctgcagcgc gtggccatcg cccgcgcgct ggtgcatcga ccgcggctgg tgctggccga
    27841 cgagccgacg ggcaatctcg atccggacaa cggggcgcgg gtgctgtccc tgctggccga
    27901 gcgggtgcgc gcgtcgggcg ccatcggcgt gctggtgacc cattccgcgg aggccgcggc
    27961 cagcgcggac cgggtgttgc gcctgactcc ggatgggctg acgggatgag tgtggccctg
    28021 cgcctggcgc cggtctttct cggggccctg gcccggcggc gaatgacgac gctgctgtcc
    28081 tgcgcggcca tcgcccttgg cgtggccctg ggcatggggg tccaggtgat ccacgaggcg
    28141 gcgctggcgg agttcgggcg ggggttgcgc agccttgccg gggcggcgga tctgcaggtg
    28201 gtgggtccgc gggacggctt tgatgaagcg ctgtacggcg tcctggcggc ccggcctgag
    28261 gtggctgcag cgaatccgct ggtggaggtg gacgtgcgtc cggccgggcg ggacgagacg
    28321 ctggctgtgc tgggtgtgga tgtctttgcc gcggccgagg tcgcgccggc cctgatgccc
    28381 caggcggacg gcggcgccgg tcgcctgggc gtgctggcgg aggatgcggt gttcctcagt
    28441 gcggccgccg ccgaagcctg gggcgccggg gccacgctga cggtacaggc gggtgcggcc
    28501 accctgagct tgcgggtggc tggcagcgtg cccggggtgg gcgccggccg gcgtctggcg
    28561 gtgatggaca tcgcggcggc acagcgcctt ttcggccggc tggggcgcct cagccggatc
    28621 ggtctgaggc ttgcggaggg cgtgtcgcct gcgcaggcgc agcgcgcgct tgcgcccctc
    28681 ctgccggcgg gggtgatcct cgaagtgcct gcagcggagg aaacgcgcag cgcgtccctg
    28741 tcgcggtcct accgggtgaa tctggccctg ctggcggcga ttgcgctgct gacgggcggc
    28801 ttcctggtgt tttcggcgca ggcactgtcg gtggtgcggc ggcgtcggga gtttgccttc
    28861 ctgcgggcgc ttgggctgtc gcggcgcagt ctgatggcct ggctggtggg cgaaggcgcg
    28921 atcatcggcg gggtgggggg cgtgttcggc gtggcgctgg gtctggggct ggcgtggatg
    28981 gctgtgcagg cgctgggggg tgatctgggg gcgggctact ttcagggcgt ggcgccacgg
    29041 ctgatcgtcg atccgctgac ctgcgcaggc tatgcggccc tgggcgtggc ggccggggcg
    29101 ctgggcgcgt ggtggcccgc gcgggaggct gcccgggtgg cgccggccca ggcgctcaag
    29161 gccgcggcgg acgaggcatt gcccggcggc agcggtcggc cgtgggcggc gctgacgctg
    29221 tggtgtctgg cggcagcggc ctgcctgctc ccgcccatcg gggcgctgcc catcgggggg
    29281 tacggggccg tggccggcat cctggcgggg gcgatcctgt tcctgccgac catcgcccgc
    29341 gtcctctccc gttgtctgcc gcggccgcgg agcgtggcgg gacgcctcgc cgtcgcccgt
    29401 ctggtggcgg tgccgggcca tgtggtggtg gccgccgctg gcgtgctcac cagcgtggcg
    29461 ctggcggcgg ccatggcgat catggtgacc tcctttcgtg agtcggtgga cgcatggctg
    29521 acccagctgc tgccagccga catctacgtg cgggccggcc gggccggcgc ggcgggtgtc
    29581 ctcgacgagg cgctgcaggc gcggctggca gcgctgaagg gcgtggtgga cgtgcgcttc
    29641 acgcgtcacg agcagctgcg cctggacggg cgcggcgcac cggtggcgct gatcgcccgg
    29701 ccggtgtcgg cggacggcgc cggcttgccg cgggtcgggg gcccgcgtgt cccgtccggc
    29761 gcgccgggcc tgtggatctc ggaggccatg gcggatctgt atggctggca gccgggcatg
    29821 acggtgacgc tgcccctggc cgggcgggcc gtgaccttgc cggtggccgg cgtgtggcgg
    29881 gattacgcgc gccagaccgg gtccgtgctg ctggatctgg aggcctaccg gcgcttcacc
    29941 ggtgaccgcc gggtccatga cgcggcgctc accctggcgc ccggcgcctc ggcaccggat
    30001 gtggcgcggg cggtggcggc gctggcgccg gaggggagcc tggaggtgag ccggccgggc
    30061 gagatccggg cgatcaccct gggcatcttc gaccggacct ttgccgttac ctacctgctg
    30121 gaggcggtgg cggtgctcat cggcctggcg ggcgtggcgg cgagctttgc ggccctggcg
    30181 gcggcccgcc ggcgggaatt cgggatgctc cgccacctgg gagtgtcacg tcgtgagatt
    30241 gccgcgatgc tggcgcggga aggggcgctg gcggccggag tgggggtgtt ggggggactg
    30301 ctcgccggcg gcgccatcgc gctggtgctg atcgaggtgg tgaatcggca aagttttcac
    30361 tggagcatgg atctgcacgt gccgtggggg tcgctggccg tgttcgcgct caccctggtg
    30421 gccctggctg cgctcgccgc ggtggccgcc ggccgccagg cgatgcgccg ggaggcggtg
    30481 tgggcggtgc gggaggaggg ctgatgcagc ggcgacggtt tctcggggcg gtgccgggcc
    30541 tggcgatcgc gtgggcaggg cgggcgcggg caggggcgga cggattcgcg cgggtggtgc
    30601 cggggcggcg cctgagtttt ccggcggatc atggcgccca cccggcgttt cgtacggagt
    30661 ggtggtatgt gaccggatgg ctgacactgc cggacgggca gccgctgggc ttccagacca
    30721 ccttcttccg ggtccggacc gggatcgggg aagacaaccc cagcgccttc gcgccgcagc
    30781 agctggtcct ggcccatggg gcgcttgccg atccgcggct gggacatctg cgtcatgcgg
    30841 aacgggtggc gcggatgggc tttggccggg ctggcttcga gacgtccgcc tgccgggtct
    30901 gggtgggcga ctggcggttc gagctggacg gcgggcgcta ccgggcccgc gtgcggggag
    30961 agggctttgc ctacgcgctg gatttcgagc cggacggggc gcccgtgctc aatggcgtgg
    31021 cgggcttcag cgccaaggcc gcggatccgc gtcacgccag ctattactac agccgcccgc
    31081 agctggcggt caccgggcag gtgacgcgcg agggtgtggc gcaacgcgtc aaggggctgg
    31141 cgtggctcga tcacgagtgg tccagcgaac tcctgccgac cggggcccag ggctgggact
    31201 ggatcggctt gaattttgac gacggcacat ccttgatggc cttccgcctg cgcgcctcac
    31261 gaggcgagcc cctgggcgcg tcggccacgc tgcggacacg cacgggcgtg cacaccgtgc
    31321 cggcgacggc cgtggccttt gagcctctgc gcacctggac ctcctcccgt accggcatcc
    31381 gttacccggt ggcctggcgc gtgcgggttg cggaccggtt gatcgagctg cagcccctgc
    31441 tggatgacca ggagctggac agtcgccgct ccaccggcgc ggtgtattgg gaaggtgccg
    31501 tgcgggcgct ggaaggcggc cgggagacgg gacggggtta cctggagatg acgggctacg
    31561 gcgatgcgat ccggctgggt gcgcgggggg aggaggacgg gtccgcgtga ccggtctgga
    31621 tgagggccgg cgcgaaaata aatttgcctg attggtctat tcgcgataat aattagaaat
    31681 ctcgagaaaa atacgggcgc caggatgggt gacaccgttg cacacgagga ggagaccatg
    31741 ggtgaacgtt atcgcgtcca catcgaaaac agcggggagg actacgtctg ccccaccgaa
    31801 cagagcctct tgcgcggcat ggaagtgctg ggccggcgcg ggattcccgt cggctgtcgg
    31861 ggcggcggtt gtggggtgtg caaggtgcac attaccgccg gcgattacga ggcgcgcaag
    31921 atgagtcgtg cctgcgtcag cgaggatgaa gagcgtcacg gcgtggtgct ggcctgccgg
    31981 gtctatcccc gcggcgagat ttccctggaa gtgatcggca agatggcccg cgcgataggg
    32041 acggcggcgc ctggtccggg cgacgtgtcc ggggtctgag tctttcagcc gggagccccg
    32101 cccccgtcat ctcacctccc tctcccgacg gggatctcca cggcttttcc tcggccgtcg
    32161 cgcaatgcga cggccgattt ttttggcgcc ggacgcgctg gcagaggccg gcggccgtct
    32221 gggcgcggtg cggtggcaat catacgggcg tcacgcaggg cttccagaat gggcgccatt
    32281 ccaactgtcg gtggggttgc ccatgaattc ctcagcagaa gcggccgtgc cttccggcgc
    32341 ggcgatccgt cccaatctgg accggccttc gggactggtc acccgctatg tcttcctggc
    32401 cgtgatggca gccggcctgc tgttcaccgg ctacagcgtg tttgccgaca ttgccgagag
    32461 cggcgcggtg gtcacgaccc tgttgccctt cgtgctcctc ggcgtggcac tgctcattgc
    32521 cctcggcttc gagttcgtca atggtttcca tgacaccgcc aatgcggtgg ccacggtgat
    32581 ctacacccat tccctgccgc cccacgtggc ggtggtgtgg tccggcgtgt tcaacttcat
    32641 cggcgtgctg gtgtccagtg gcgcggtggc cttcgggatc atctccctgt tgccggtgga
    32701 gctgatcctg caggtgggct ccagcgccgg cttcgcgatg gtgttcgcgc tgctcatcgc
    32761 ggcgatcctg tggaacctgg gcacctggtg gctggggctg ccggcgtcct cgtcccacac
    32821 cctgatcggc tccatcatcg gcgtcggtgt ggccaatgcg ctgatgcacg ggcgtgacgg
    32881 taccagcggc gtggattggg cgcaggccac caaggtgggc tattccctcc tgctgtcgcc
    32941 gctggtcggc ttcggttgtg cggctttgct gctgctggcc ctgcgggccc tggtgaagaa
    33001 ccgggcgctc tacgaggagc ccaggagcga cgcgccgccg ccctggtgga tccgcggcat
    33061 cctgatcctg acctgcaccg gtgtgtcctt tgcccatggg tccaacgacg gccagaaggg
    33121 catgggcctg atcatgctga tcctggtggg caccgtgccc ctgtcctacg ccctcaaccg
    33181 ggcaatgccg gcgagtcagg tgacccagtt cgccgcggtg gcccacgtca cccaggacgc
    33241 cctggtcaag gctgcgccgg tggcgccgcc ggccgagccg cgcaaggtgc tgtcggacta
    33301 catccggacc catgaggtga caccggaggt ggtgccggcc ctcgccgcgc tgaccggcac
    33361 catcggtgcg caggtgaagg attatccgtc cctggccagg gtgccggcgg aatccgtgcc
    33421 caacgtgcgc aacgacatgt acctggcgtc ggaggcgatc cgctttcttg aaaagagcga
    33481 ccaggtgaat ttcgacgccg acgccaaggc caacctgaag gccttcaagg gccagctcga
    33541 tacggcgacc aagttcattc ccctgtgggt gaaggtggcg gtggccgtgg cgctgggcct
    33601 gggcaccatg atcggctgga agcgcatcgt cgtgaccgtc ggcgagaaga tcggcaagtc
    33661 gcacctgacc tacgcccagg gcgcgtccgc cgagctggtg gcgatgacca ccatcggtgc
    33721 cgccgacctg ttcggcctgc cggtgtccac cacccacgtg ctgtcatccg gcgtggcagg
    33781 caccatggcc gccaacaagt ccggcctgca gatggccacc atccgcaacc tgctgatggc
    33841 ctgggtgctg accctgccgg cggcgatcct cctgtcgggc agcctgtact ggctgttcgc
    33901 ccacctgttc tgagccgtac gaggaatgaa agcatgagca catcgcaccc ggtcgatcag
    33961 gaggaactgt tgcgccgtct gcagcgcgac ctcatcgatg gctacgacga agaactggag
    34021 atggagatcg aggaccggcc gtggaccgat ccaatgcagc cgattgccgt cgatgaagcc
    34081 gaggccgagg cccaccgcca gcagcgccac cgctactttc aggatctgtt ccgcctgcag
    34141 ggtgagctgg tcaagctgca ggactggatc gccaccaccg gccagcgtgc cgtgatcctc
    34201 ttcgaggggc gcgatgcggc gggcaagggg ggcgtcatca agcgcgtggt gcagcgcctc
    34261 aacccgcggg tgtgccgcac cgtggcgctg ccggcgccgt ccgaccggga gcgcacccag
    34321 tggtatttcc agcgctacgt ggcccatctg ccggcggccg gtgagatggt cctcttcgac
    34381 cgcagctggt acaaccgggc tggcgtggag aaggtgatgg gcttctgctc ggacgacgag
    34441 tacgaggagt tcttccgctc ggtgccggaa ttcgagcgca tgctggtgcg ctccggcatc
    34501 aagattgtga agtactggtt ctctatcacc gacgacgagc agcagctgcg cttcctgagc
    34561 cgcatccacg atcccctcaa gcaatggaag ctgagcccca tggacatgga atcgcggcgt
    34621 cgctgggagg attacacccg cgccaaggag atcatgctgg agcgcaccca tatccccgag
    34681 gcaccctggt gggtggtcca ggcggtggac aagaagcggg cgcgcctgaa ctgcatccac
    34741 catctgctgc agcagttccc ctacgaggag atccagcgcc cgccggtgct cctgccagcg
    34801 cgggaacgcc atgaggacta ccagcgcagc gccgtccagt cggacctgtt cgtgccggag
    34861 atctattgag cgtgatggac gacgacgatg ccccggtaca acggtggggc acggggcgcc
    34921 gatgagttcg gccgccctca ccgcctatgg cgcggaccct tcggggctgg tgtatggcta
    34981 cgtgttcacg gcggacggcg tcggccgcca ggtggacacg gcggcggcgg gcgcctggct
    35041 ggccgagcgg gatgcccgtc cgggcgaatt catctggttg cacctgaccg gtgtacaggc
    35101 cgccacgatg aattggctgc agagccacct ggaactgtcg gaggatttca ccgatgccct
    35161 caaggaaggc tcccgcagca cgcgcatcga gctggtccat gccagcctga tcgccgtcat
    35221 caacgacgtg gtctatgacc tgagccgcga gaacaccctg caggtggcga ccctgtggat
    35281 gaacgtgacg ccccgctgcc tggtgagcgt gcgcatccag cccctgcgct cggtggatcg
    35341 gctgcgcgcc gcggtgaagg cgggcgagac cttcggcagc ccgctggcgc tggtgatcca
    35401 cctgctgcgg gaacaggcgg acatgctggt gcacatcatc cgcagcgcca ccgagaaggt
    35461 ggacggggtg gaggacaagt tcctcctcgg gcgcctgccc gagcgggcag cgctgggcgg
    35521 catccgtcgc gacctggtgc gcctgcagcg tctgctggcg ccggagccgg ccgccttgtt
    35581 ccgtctgctg aaccgtccgc cggcctgggt gagcgccggt gacacccagg atctccagca
    35641 gtcgacggaa gagttctccg tggtgctgcg ggacatggcc ggcctgcagg agcgcatcaa
    35701 gctgctccag gaagagatcg tcgcccacat cggcgagcgc accaaccgca gcgtcttcgt
    35761 gctgaccgcg gtgaccgtga ttgccctgcc catcaacctg acggcgggcc tcttcggcat
    35821 gaacgtgggt ggcattccgc tggcggagga tccggaaggg ttctggcggg tgctcctgct
    35881 ggtgctgctg atgaccgccg gtgcggcctg gtacgccttc cggcgcgggc cggagtaggg
    35941 tgccggccca gcggccgggc gtggcgtgcc ggacgcgcct ccgcgccggg cggccatccc
    36001 tgcaaggggg ctttcaggcg aggggctttt cccgcttcag gcggtagctg aactggggtc
    36061 gggtcaggcc cagcagccgc gcggcaccgg acaggttgcc ctgctggcgc tgcatggcct
    36121 cgcggagcag ggcggcttcc aggtcgtcga gggaaatccc cgacgcgaac actgcgtcgc
    36181 acagggcgct gacgtccggg gcggcgtccg ctgtcgccgg gcgcaggccg ccgctggcgt
    36241 ccacgcgatc ctcgctgcag ggcgaaatgt cgaggcccgc gaagaggtgt tcggcctcga
    36301 tccagccgtc gtgggcggcg aggatcacgc cccgttccat catgttctcc agttcccgca
    36361 cgttgcccgg ccacttgtgg gccttgaggg cgcgcagggc cttgtcggtg acgcccttga
    36421 gtcgcttggc gtggacggcg cagaagcgct ccagcatcgc gtgggccagg ggggggatgt
    36481 cagagacccg ctcgcgcaag ggggggatga ccaccgggta gacgttgagg cggtaataca
    36541 ggtcgctgcg gaagcggccg tcccgcacgg ccgcctgcag gtccacgttg gtggcggcca
    36601 ccaggcggat gttgatcttg cgcgggcggt cgtcgccgag gcgctccacc tcgccgtcct
    36661 ggagcacgcg caggagcttg gcctgggcgg cgaggggcat ctcgccgacc tcgtcgagga
    36721 acagggtgcc gccgtcggcc cgctcgaact tgccggcgcg ggaagcgtgg gcgccggtga
    36781 aggcgccctt ctcgacgccg aagagctctg actcgatcag gtcgtggggc agggcggcgc
    36841 agttgacggc gacgaagggc gcctcccggc ggctgcccag ttcgtgcagg gcgcgggcga
    36901 agcgctcctt gccgacgccg gtctcgccca gcagcagcac ggtcacctgg gtgggggcgg
    36961 ccttcttgat gagggtgtag gcgtgctgga agctcgccga ggcaccgatg aggctgggga
    37021 tctggtggct ggcgccgagg gtcgagcgca gggtgtccac ctcgtcgcgc aattccagga
    37081 tgcggccgac gacggcggtg tcgtcgtagt agcgcagcag ctcggcggcg tcgggccatt
    37141 cttcggccgg cttgccaacg atgcggcagg tgttggcgcc tgcggccgcg cattccacct
    37201 cgcggaacag gatgaagcgc cccatgaagc aggacgaata gcccgaggca tagccgatct
    37261 gcatccagca caccgggtgg tcggcggggc cgaattcccg tacatgggcg tccacttccc
    37321 aggagttctt ccacagcagt tcgccgtaga aactgccggt gtccgggttc atgtcgaggc
    37381 ggatcggttc gacctgcacg ctgccttcca gcatgtggag ctgggggccg acgcagaagg
    37441 cgtcgagcat gtcacgggag ccgcgcacct tgcgggccag ctcggcgtca cgcatgccgg
    37501 cgccgaagcc catgcgggtc agcacgcgac gggcctggtc ctcgccgacc gagtggatga
    37561 gctccttgcg cagggcctgc atggtggtca cctggagcag cagcatgcgg ctctcgccca
    37621 gccagatcag gccgtcgtcc gccgaaaagc ggatgagctc ccgcaggtcc ttgttctccg
    37681 ggtacttgat ggtcaccgtt gtgtctcctc cgtacgccgt gccggcgcgc atgggtggtc
    37741 ccatgtctga tgtcgcccgt ccggggatcg gggcgggggt ggcggggcct gtgccgcacc
    37801 agagggggtg gtgggcagat tgtcgctgcc gctatcgtac aaaatctcga gattttatgc
    37861 aatccaccgg acgatatgga tccgacgtga tcgcggttcc acgacgccgt gtgaggtttc
    37921 aaggccgtgg ggcctgcccg ccatcctgcc ggagattgtc ctccgatagg cgtgacgcag
    37981 tgtgcatctg tgtgacgtgc agcccgtgcg tggcgcttcc gtgccctgca gcggacgtcc
    38041 gagcgcttgg ccgcgcggcc atgttgcggt gcgcaagtct ttgttgcaac gcgaaaaagt
    38101 ggcttcacga tttcagcagc cagggttgaa ccatttaatc aattgatcaa tgcaatcgca
    38161 gtgtttatcc gcaaatctcg agaatattat gtttaatccg agataaatca gcagcttgcc
    38221 gacactggca cagggcttga ttacatgggg ccggactcga caaaagtgaa tcaaggggac
    38281 gaccgacatg aaatcagaca cgccggcagt cgatgtgaac cggaagttcg tgcgcctggt
    38341 ggaacgacgc gcgaacgggc tggtggagtt cgagttctcc atcggcgatc cggagctgtt
    38401 cgtggaaatg ctgctgcccg ccaccgcctt cgaggagttc tgcgcggtga atcgggtgga
    38461 aatccttgaa gggtcccgtc cgcacgaggc cggttccgac tgggactggt ccctgcacga
    38521 ggccacgcac cagcgcttcc gctgaggtcc gcagccgcgg acatccaaca caagacacag
    38581 gaggagacac catgcagatt gacttgagga ccgtggcgat caacccgctg cgccagacct
    38641 atgaccacat cgcccgacgc atcgggccgg acaaggcggc gtcgcggtac caggaaggca
    38701 ccctggacct gcaggccacc cacaacttcc attaccgccc gacctgggac cccgatcacg
    38761 agatcttcga cgcctcgcgc tcggcgatcc gcatggccga ctggtacgcc ttcaaggatc
    38821 cgcgccagtt ctactacggc gcttacaccc tggcccgcgc caagcagcag gacaccgccg
    38881 aggcgaattt cgactttgtc gagaaccggg gccttgccgc gaccctgccg gaagacctgc
    38941 gcgccaccgt gctggcggtg ctgctgccgc tgcgccatgt ggcctggggc ggcaacatga
    39001 acggcgcctt catcgccgcc tacggctatg gcacggcgct gacccagccc agcctgtacc
    39061 acgcgatgga tcagctcggc atcgcccagt acctcacccg cgtcggtctg ctgctgggcg
    39121 acgacccctc cgtgctggac gacgccaaga cgcgctggat ggaggacggc gtgtggcagg
    39181 gcctgcgccg ctacgtggaa gacaccttcg tgctgaagga tccgttcgag ctcttcgtgg
    39241 cccagatggt ggccctggac ggactgctct acccgctggt gtacgaaacc atcgtcgatg
    39301 acgtcctctc cgccaacggc ggcactgccg tggcgatgct cacccagttc atgaccgaat
    39361 ggttcgccga gacccgcaag tgggtggacg ccaccgtcaa ggccgcggcc gccgaatctc
    39421 ccgagaacaa ggcgctgctg gaaaagtgga tcggccactg gcgcgaccgt gcggccgctg
    39481 ccctgatgcc agtggcccag atcgccctgg ggtcccaggc cgacgcggtg accagcgagc
    39541 tcgtgtccca gttcaacacc cgcctggcca aggccggcgt cagcctgtga gaggaagcgg
    39601 catgtccaag gtattcatcg ccctgcaaac caatgacgag acccgccaga tcatcgacgc
    39661 gatcctcgcc gacaaccccc acgccatcgc ggtcaatcag cccgccatgg tgaagatcga
    39721 tgcggagggc tccctcgtgg tacgccgctc caccatcgag gaactcctcg gtcgcgacta
    39781 cgacctgcag gaactgcaca tcaacctgat ctccctcacc ggtcacgtgg acgagaccga
    39841 cgaggagctg accctcagct ggaacgcctg agacacacgg agacacgcac atggacatga
    39901 aagtcagcaa gaagaagctg ggcctcaagg aaaagtacca gcacatgacc cgcggcctgg
    39961 gctgggagac cagctaccag cagatggacg acgtgttccc gtacgccaag tacgagggca
    40021 tcaagatcca tgactgggac aagtgggaag acccgttccg cctcaccatg gacgcctact
    40081 ggaagtacca ggccgagaag gaacgcaagc tctacgcgat catcgacgcc tacgcccaga
    40141 acaatggcca cctgtcggtg accgatgccc gctacatcaa cgccatcaag ctcttcctca
    40201 acggcatcag cccgctggaa tacatggcgc accggggctt cgcccacgtg ggccggcagt
    40261 tccccggcgt cggaccgcgg gtcgcctgcc tgatgcagtc cctggacgag atccgccacg
    40321 cccagacgca gatccacgcg ctgtccaatt acaacaagta ctacaacggc ttccacgaat
    40381 tccgccacat gatcgagcgg gtctggtacc tgtcggtgcc gcgctccttc ttcgacgatg
    40441 ccgtcaccgc cggtcccttc gagttcatgg tggccatcgg cttcagcttc gagtacgtgc
    40501 tcaccaatct gctcttcgtg cccttcgtgt cgggtgcggc ctacaacggc gacatgggca
    40561 ccatgacctt cggtttctcg gcccagtccg acgaggcccg ccacatgacc ctgggcctgg
    40621 aggtcatcaa gttcatcctc gaacaggatc ccgacaacct gcccatcgtg cagcgctgga
    40681 tcgacaagtg gacctggcgc ggctaccgcg tgctgaccct ggtgggcatg atgatggact
    40741 acatgctgcc caagcgcatc atgagctgga aggaagcctg ggaggtgtat ttcgaggaga
    40801 acggcggcgc cctgttcaag gatctcgccc gttacggcat ccgtccgccc aagtggcttg
    40861 atcagatcgc ttttgacaag gaccacctgt cccaccaggc ctgggccacc ttctaccagt
    40921 acggcgccgt gtgcaacttc cacacatgga tgcccaccga cgacgagatg gactggctgt
    40981 cgaagaagta cccgaacagc ttcgacaaat attaccggcc gcgcctggag ttctggcgcg
    41041 acgaggccgc caagggcaac cgcttctaca acaagacgct gcccatgctg tgccagacct
    41101 gccagatccc catgctcttc accgagccgg gcgatcccac ccagatctgc tatcgccagg
    41161 tggactacaa ggacaacacc taccacttct gctcggacgg ctgcaaagag atcttcgagc
    41221 acgagcccga gaagtacatc caggcctggc tgccggcaca gcagatctac cagggccact
    41281 gcttcaacga aggggtggat cccacggccg agggcttcga cccgctgggc gcggtgctcg
    41341 actactacca catggccgca ggccgcgaca acttcgactt cgacggttcg gaagaccaga
    41401 agaattttga agcctggcgc gggctggcca ccaagaacgc gtgacgggcg cggggcgggc
    41461 gcacgtccgt cgttccgctc cggcccatcc acaccagaca cgaggagaca tcaccatggg
    41521 cgttgcagcc atcaaggact acaccttcga gcccgccgac cggctcgaga acttccacgg
    41581 caaccagttg ctgaacatcg gctgggacca gcacctgctg ttctgtgcgc cgttctgcct
    41641 gcccctgccg ccgacgacgc gctttggcga catcgtcgac aagatcctgc cgggcatctt
    41701 cggctatcac ccggacttcg cgaagatcga ctggagcacc gtgcagtggc tcaagggcgg
    41761 tcagccctgg cagccggatt tcgccaggac ccttgcggaa ctggggctca ggcacaagga
    41821 cgcgctgcgc ttccgcacgc cggggctcaa cggcatccag ggctcggcga gctgagcatg
    41881 ggctacgaac tgaccatcga gcccctcggc cagaccatcg aggtggagga ggggcagacc
    41941 atcctcgatg cgtccctgcg cgccgggatc tggctgcccc acgcctgctg tcacggcctg
    42001 tgcgccacct gcaaggtgca ggtggtggac ggcgaagtgg atcacggcga agcctccagc
    42061 ttcgccctga tggacttcga gcgcaccgaa ggcaagacgc tggcctgctg cgcccgcctg
    42121 gaaagtgacg tgaccatcga ggcggagatc gacgaggagc cggatgcgga atccatcccg
    42181 gtgaaggact tcagcggcac ggtgagccgc atcgaaaccc tcacgcccac catcaagggg
    42241 ctgttcttta ctctgaacga gccgctccat ttccaggccg ggcagtacgt caatctggag
    42301 atcccgaccc tgggcatcag ccgggcgttc tccctggcca acgtgccctc cacggggcgc
    42361 gaggtggagc tcaacgtgcg catcgtcccc ggcggcgccg gcacgggcta tctgcacgag
    42421 cgcctgaagg tgggtgaccc gatccgcctg tccggcccct acgggcgctt cttcgtgcgc
    42481 aagtccgcgc aggtgccgct gatcttcatg gcggggggct cgggcctgtc gagtccgcgc
    42541 agcatgatcc tggacctgct ggctgaaggc agcaccctgc cgatcaccct ggtgtacggc
    42601 cagcgccgcc gtgaggagct gtattaccac gacgagttcg tcgagctcgc cgcgcgcttt
    42661 cccaacttca gctacgtacc ggccctgtcg gacgagccgg cggattcggg gtggacgggc
    42721 catcgcggct tcgtccatga ggccgccaag gcccatttcg acaacgactt ccggggtcac
    42781 aaggcctacc tgtgcggtcc gccggccatg atcgacgcgt gcatctccac gctgatgcag
    42841 gggcgcctgt tcgagaacga catctatacg gagaagttct tcagcgccgc cgatgcccag
    42901 caggtgcgca gcccgctgtt caggcgtgtg taatctgcgc cttaatttgt cgatagaaaa
    42961 ataaaatctc gataaaaaat tgacttgtcg agaagctgca ttaaactagc aaccagttcc
    43021 gccgagccca caggaggcca ccaagccccg tcggcggaag actctcaaaa ggaagaggag
    43081 acaatcatgg caatgactgg cgtgctgcgc cccggccaca tgcaactgcg cgtgctggac
    43141 ctggaagaag gcgtgaatca ctacaagaac gtg
//
LOCUS       DAOQPA010000012        41940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_369_length_41940_cov_20.572424, whole genome shotgun sequence.
ACCESSION   DAOQPA010000012 DAOQPA010000000
VERSION     DAOQPA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 41940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41940
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_41940_cov_20.572424"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKX01_03100"
     CDS             complement(<1..379)
                     /locus_tag="PKX01_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75138.1"
                     /translation="MSEPQIPADNYDESSIQQLEGLEAVRKRPGMYIGDTSDGTGLHH
                     MVFEVVDNAIDEALAGHCDDIVITIHADNSISVTDNGRGIPTGVKMDDKHEPRRSAAE
                     IVMCVLHAGGKFNQNSYKVSGGLH"
     gene            complement(579..1685)
                     /gene="dnaN"
                     /locus_tag="PKX01_03105"
     CDS             complement(579..1685)
                     /gene="dnaN"
                     /locus_tag="PKX01_03105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ75139.1"
                     /translation="MLLFTAPRDALLAPLQSVSGIVEKRHTLPILSNVLIEKKGEALT
                     LLATDIEIQIRNRTTAALGAEDVAITVGARKLQDILRALPDSAEVQLTLDDKRITLKA
                     GKSRFQLQTLPAADYPRLAPPEGEGVHFSTTQKAFKKQLALVQYAMAQQDIRYYLNGL
                     LLVVAGGTLRMVATDGHRLAYAASAIEGDYPRTDVILPRKTVVELARQLADSDDPVEV
                     TLVGNQIVFRFGHIELISKLIDGKFPDYERVIPQHHPKLVKASRLALLQSLQRAAILT
                     NEKFRGVRLILSEGSLKLVSTNAEQEEAQEELEVDYSGDALDIGFNVTYLLDVLTNLS
                     ANEIEIRFNDNNASALFGLGDNPDFKYVVMPMRI"
     gene            complement(1749..3218)
                     /gene="dnaA"
                     /locus_tag="PKX01_03110"
     CDS             complement(1749..3218)
                     /gene="dnaA"
                     /locus_tag="PKX01_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMZ75140.1"
                     /translation="MSEASSIPPSTAFWNHCLGCLEQELPAQQFKTWILPLRAEDLAE
                     GDAAGLRLVAPSNFHRQWLRDRYLRRIEELGEDYFGAPPVIEVGIAPAGGLRRPAAPP
                     PGPGSATSGIESVSTPATAGVGIATPVAPAVHTTRAETPLTAEAANYEKSRLNPDFTF
                     DTLVTGRANDLARAAAMQVAQNPGVSYNPLFIYGGVGLGKTHMIHALGNTVFKADPRK
                     VIRYVHAEDYYADVVRAYQQKSFDVFKRYYRSLDVLIIDDIQFFNNKNRTQEEFFHAF
                     NALTEAKKQIIITSDTYPKDVQGLEDRLISRFDWGLTVQIEPPELEMRVAILKKKAEA
                     ERISLDEDVAFLIAKNLRSNVRELEGALKKVLAFARFHGRDVTLEVAKDALKDLLNAH
                     NRQLTVEFIQKTVADYYKIKVADMHSKKRTRVIARPRQVAMALAKDLTPMSLPAIGEA
                     FGGRDHTTVLHACRTIAELRLGDTQLNHDLHVLTQVLRG"
     gene            3571..3705
                     /gene="rpmH"
                     /locus_tag="PKX01_03115"
     CDS             3571..3705
                     /gene="rpmH"
                     /locus_tag="PKX01_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMZ75141.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMATRGGRKVIAARRAKGRHRLGV
                     "
     gene            3728..4102
                     /gene="rnpA"
                     /locus_tag="PKX01_03120"
     CDS             3728..4102
                     /gene="rnpA"
                     /locus_tag="PKX01_03120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMZ75142.1"
                     /translation="MNGAATEDSGGPAWGFGPSLRLHKTDEFSSVFAFRRVVRGRYFA
                     LHYAPCRTDEPRLGLVVAKKLARRAVLRNLVKRIGRDVFRHAHASLPRYDVVLRLSAP
                     LASVTKRQMREDMLGLFGRLPR"
     gene            4099..4308
                     /gene="yidD"
                     /locus_tag="PKX01_03125"
     CDS             4099..4308
                     /gene="yidD"
                     /locus_tag="PKX01_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMZ75143.1"
                     /translation="MKWLVLACLRTYRYAISPLLGRNCRFFPSCSEYALEAVERHGAL
                     HGGWLGLRRVCRCHPWSAGGYDPVP"
     gene            4399..6048
                     /gene="yidC"
                     /locus_tag="PKX01_03130"
     CDS             4399..6048
                     /gene="yidC"
                     /locus_tag="PKX01_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMZ75144.1"
                     /translation="MDTRRLILLLVFSFSMVMLWDGWQKHTHPAQPATAASGTAPASN
                     GVPTPTAATTVSAPGVPGVPQIASPAAAAPRMKVRTDVLVADVSAEGGDIVRLELTRH
                     KATGDAEHDYVLFDDGEKHLYAAQSGLIGNGLPTHKTRFALPAQERVLKDGEDAVSVR
                     LEAPATADGIRVSKLITFHRGSYVAEVSYEIANGSGAPIAPHAYFQFMRDGKPAEQAG
                     GFGVQTFTGPAFYTDAEKYQKVNFQDVTDKKAKFVTKAPDGWVAMVQHYFVAGWAPAA
                     GEREFFANALGGDLFAAGVIVPVAAIAPGQSGTVSVPLYAGPQEQSRLDQFAKGFDLV
                     VDYGWLTIIAAPLFWVLEWFHKLTGNWGWAIILVTIALKALFFPLSAASYKSMAKMKT
                     LGPRLQRMKEQFGDDKMKLNQAMMDLYKREKVNPLGGCLPILIQIPVFIALYWVLLGV
                     VEMRGAPWLGWIQDLSVKDPYYILPLIMGATSLIQTKLNPAPPDPIQAKVMMMMPILF
                     TFMFLWFPAGLVLYWVVNNCLSIAQQWQINRMIEAGGKAAE"
     gene            6029..7378
                     /gene="mnmE"
                     /locus_tag="PKX01_03135"
     CDS             6029..7378
                     /gene="mnmE"
                     /locus_tag="PKX01_03135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMZ75145.1"
                     /translation="MARPPSELIAAIATAPGRGGIGVVRLSGSDLAPLARILGGGDTQ
                     PRTARFCRFRDAAGGLIDEGLLLYFPAPHSFTGEDVIELQGHGGPVVMQLLLGRCLEL
                     GARLAEPGEFTRRAFLNGKLDLAQAESVADLIEASTTAAARSAVRSLSGAFSSEVHAL
                     RDRLIDLRMLVEATLDFPDEDIDFLVEARAFERLAVMEGELAAILDRAKQGSLLRNGL
                     HVVLVGQPNVGKSSLLNRLAGEERAIVTDIAGTTRDALRETIQIEGIPLHITDTAGLR
                     ETSDEVEKIGIARTWQEIARADVVVRLVDVRAGLTEADAAIDARLPAGVERITVFNKV
                     DLSGRASERVEQGDIGVSLYLSAKAGEGVDLLRTELLRVAGWHRHGEDVILARERHLI
                     ALREARARVSAARDAGHALELLAEELRLAQDAINAITGEFTPDDLLGVIFSRFCIGK"
     gene            7514..7717
                     /locus_tag="PKX01_03140"
     CDS             7514..7717
                     /locus_tag="PKX01_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HMZ75146.1"
                     /translation="MKKLTIGLAIVAAVSFSGCANMNAQQRNTAIGAAVGGVAGSILT
                     GGSSIGTVGGAAVGGVIGNQVKH"
     gene            8199..8960
                     /locus_tag="PKX01_03145"
     CDS             8199..8960
                     /locus_tag="PKX01_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ75147.1"
                     /translation="MAWEPERYLQFSDERLRPAVDLLGRVTLASPARIVDLGCGAGNV
                     TRLLQARWPDAELRGVDNSPEMLARARQALPSAQFELAAVEDWQPDAPVDLVFSNAAL
                     HWVGEHHHLFPRLMGAVAQGGVLAVQMPGNFAAPSHRLICELAESDAWRERVGRGRMG
                     AILEMDAYFRVLSPWAQTVTVWETTYWQALSGAAPVLDWLRGTTLVPYLAPMSDLERE
                     AFLAQLGARLAVAYPKDEQGRTLFPFRRVFLVAQR"
     gene            8957..9787
                     /locus_tag="PKX01_03150"
     CDS             8957..9787
                     /locus_tag="PKX01_03150"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HMZ75148.1"
                     /translation="MTQALFEAAADLIAQADGLIIAAGAGMGVDSGLPDFRGNQGFWR
                     AYPALAEARIAFEEIACPDAFRDAPERAWGFYGHRLNLYRRTIPHAGFAVLKRWGLRL
                     PRGAFVFTSNVDGQFQRAGFDSNQVCEVHGSIHHLQCVQGCDGRILSAEGWSPEVDEV
                     GCRLLSPLPRCSVCGDLLRPNVLMFGDWAWLPQRTQSQRVRLEQWLAVVDRPVVVEIG
                     AGSHVATVRWFSESMGVPLVRINPTEPEVPGRGVELPMEGLRALSEIDAVLAARGALR
                     "
     gene            9815..10579
                     /locus_tag="PKX01_03155"
     CDS             9815..10579
                     /locus_tag="PKX01_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HMZ75149.1"
                     /translation="MDATYLILGLGAFFAGCVDAVVGGGGLIQIPLLFTTFPTQGTAT
                     LFGTNKLASVVGTSSAAWRYARRIAVPWKVIAAAALAAGAGSWIGARAVAYFPPAALR
                     PVMLALLIAVALYTFVRKDFGVRPDTSASPSDPWRRAVFIGAGVGFYDGFFGPGTGSF
                     LIFLFVRALNWDFLRASVAAKIVNMVTNVAAIGFFVSHGEVLWRVAAVMALCNLCGAQ
                     LGSGLALRHGAPFVRRAFLLVACVLIVRFALDTFRI"
     gene            10591..11460
                     /locus_tag="PKX01_03160"
     CDS             10591..11460
                     /locus_tag="PKX01_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HMZ75150.1"
                     /translation="MKQSLQHLLIPLGDGVRLEVERTAESVELHSRWQTIRTEHTARF
                     GELYCLDGDAMVSTADEFMIHESLAHHVALEHTSPRRALILGGGDGGTARELLKHPCI
                     DAIVIAELDERVVARVREALPTVSAGALDNPRVTLRLGDAGETLEASQQCGERFDLIL
                     FDLTASDDPACAHLHAPPFLNACADRLMPGGRLHVQLGSPFYQPEAVAALHRRLNGVF
                     PYVRPALISVPLYGGPWLLAAASVEALPETSEAQLAQRLAERAIPPLRYYNPALHRAS
                     FALPNDVHALLTA"
     gene            11464..12783
                     /gene="rsmB"
                     /locus_tag="PKX01_03165"
     CDS             11464..12783
                     /gene="rsmB"
                     /locus_tag="PKX01_03165"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HMZ75151.1"
                     /translation="MPISPRRSSPARPLPEDSLAHALCCAAELIARVAGGAALTDILA
                     DLWVSRPATPPAVRGAIQDLTYGTLRDYGRQDFILGRLLDKSLPEPVQSLLRVALHRL
                     DVRPESAHTVVDQAVNAAATLLSGRFKGVVNGVLRNALRQSATLRYAADADLVAEMRH
                     PPWWIRRVRDQHPQDWRNVLAAGNVHPPMALRVNRRRSTPEAAAAELAEAGLSARTWP
                     GGALLLERPVPVVRIPGFAEGRVSVQDIGAQAAAPLLDVASGQHVLDACAAPGGKTAH
                     LLELADVDLLALDADPQRARRIDENLQRLGLSARVMAADCRAIDRWWDGRPFDRILAD
                     VPCSASGVVRRHPDIKWLRRSGDIGRFAAQQAEILDALWQVLAPGGKMLYATCSVFRE
                     ENQDQVARFAASHADCHRLTIEGQTDRQFLPCADHDGFFYALLHKAA"
     gene            12743..13360
                     /locus_tag="PKX01_03170"
     CDS             12743..13360
                     /locus_tag="PKX01_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HMZ75152.1"
                     /translation="MTDFSTPCSTRLPERRRFLHRLSAVAGGVVLGGLAMPAWTAEAD
                     IRSASLVLTDDLYVLHADIVAELGSLLGDVLMRGVSLFFVVEFQLTSSRWYWLDEEIA
                     NRTLTYRLSYQALTRQYRLSVGALHQNFMTLDEALRTMLRVRNWGVLDRAAVKPGHSY
                     NAAMRFRLDVAELPKPLQVSAFGRPDWNLSSDWLRWTFVPQRDAK"
     gene            13357..15492
                     /locus_tag="PKX01_03175"
     CDS             13357..15492
                     /locus_tag="PKX01_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75153.1"
                     /translation="MRPTALVVAAAVAGILLFLLATASANTPLFARNYTLLLGLNGAI
                     TVGLVALVFVQLRSLWQEHRAHLFGSRLKLRLMAIFAAVAVVPGVLVYAVSLQFAVKS
                     IESWFNVRVDAALDSGMQLGRTTLDFLLDQLAEKGGQMAIELETGGPIRATRLNQLRE
                     QAGVDSATVFAGNGRVQVTSESQMGELVPDYPSPTQLKQARDTRGYRAIEGDAASGLL
                     LRVILPIPSRHLSAEAPLLQVTQEVPRNFAIHAESVQNVYRDYQELSLARNGLKRIYT
                     LTLTLTLLLALFGAVAAAFVLTRRLAAPLLILAEGTEAVAAGDFSPRRALPARDELGV
                     LTQSFNQMTRQLEEARGLAEKNRAAVETSRAYLEGVLANLSAGVLAFSPEGLLRAANH
                     GAMTILNDRLEGCERTPLEAWPAHPMFRDALLRGFHGSDPDWHEQLEVTHADGSTQTL
                     LLHGTRLPADTGGGWVVVFDDITQLIAAQRNAAWAEVARRLAHEIKNPLTPIQLSAER
                     LQFKLAERLDDEGREMLERSTRTIVNQVESMKNLVNAFRDYAKLPAPVLVPLDLNALV
                     REVLVLYEGSRVMIRAELAAELPPVLGDATQIRQVIHNLLQNAEDALVDEPDPEIVLL
                     TKSDARRVDLLLRDNGTGFPPDLLSRAFEPYVTTKSRGTGLGLAIVKKIIDEHSGEIR
                     LANLESGGAEVRIRFRSSESLDHGKNPDR"
     gene            15470..16720
                     /locus_tag="PKX01_03180"
     CDS             15470..16720
                     /locus_tag="PKX01_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75154.1"
                     /translation="MAKILIVDDEVGIRELLSEILRDEGHDIILAENAAAARAARNAA
                     RPDLVLLDIWMPDTDGVSLLKEWSGNGQLTMPVVMMSGHGTIDTAVEATRIGAMEFLE
                     KPIALQKLLAAVKRGLARVKPTHAPSPLGLAAFSRSPALRDLRKRLEQVAEKSRIILL
                     RVGPGSLAELCARTLQRAGTPWLDLSLTTMPLELNTLESARNGVLYIPELIYLSRPQQ
                     KNLVFAMDRLERHGQTLVVATEHRPEELVEHGWDERVVLRLFEVSLAPPALSEIRDDI
                     PEIAGQILLHYVEAGEVPSRRLSSAALNALRNQNWSGGYSELKAVVKSLALGTLEDEI
                     SMDDVAHLLVKAPSTAFGLPLDLPLREAREMFERLYFEHHLQLDGGNMTRLAEKTGLE
                     RTHLYRKLKQLGISVGRRHEEASS"
     gene            16830..17903
                     /gene="hemE"
                     /locus_tag="PKX01_03185"
     CDS             16830..17903
                     /gene="hemE"
                     /locus_tag="PKX01_03185"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HMZ75155.1"
                     /translation="MSRPKNDTFLRALLRQPTDYTPVWLMRQAGRYLPEYRETRKRAG
                     SFLDLCKNPSLACEVTLQPLARYNLDAAILFSDILTVPDAMGLGLYFSEGEGPRFERP
                     LKEEWEIRNLAVPDPNLELGYVMDAVSEIRRALDGSVPLIGFSGSPWTLACYMVEGGG
                     SDDYRKIKTLLYTRPDLLHHILDITAQAVTAYLNAQIEAGAQAVMIFDSWGGVLAHDA
                     YKTFSLAYMRRILAGLIREKDGERVPAIVFTKGGGLWLEDIAASGTDAVGLDWTVDIG
                     AARRQVGDRVALQGNLDPSVLFGSPEAVAEEARRVLDAFGPHPGHVFNLGHGISLHTP
                     PDNVSVLVQTVHAHSRALRQQAE"
     gene            18428..20596
                     /locus_tag="PKX01_03190"
     CDS             18428..20596
                     /locus_tag="PKX01_03190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMZ75156.1"
                     /translation="MHVVRVALPVPLPQLFDYTSENVSESDVGRCVRVPFGPAEKNGV
                     ILAIDPPDGITPERLKSVLAIQRDAPALPADWLALVDFAARYYQHPLGEVIALALPPR
                     LRRADAVAGDEVDPLLDVTPDGHLALGRSTRESRALAVVRRLAAEGAVRRAALRAAEG
                     AAVADALRRGWLAASAADEPARMATAPELTEEQRAAVARLADGFGRYQPFLLVGVTGS
                     GKTEVYLRLVREAITRGGQALILVPEIALTPQLEARVAGRFPAARVVSLHSGLGEAAR
                     SQGFVQALEGRADIVLGTRLAVFTPLPRLALIVVDEEHDPSYKQQEGVRYSARDLAVW
                     RGRQRGVPVVLGSATPSLESWHAAEQGRYGRVELTRRAIAATLPAVRTLDVRREKLDH
                     GLAPRLLEALDVRLQRGEQSLVFLNRRGYAPVLSCPSCGWVSRCLHCSANMVLHLTDR
                     RLRCHHCSFETGLPVACPDCGDVDIQPFGRGTQRVEEVLTERFPAARVVRVDRDSART
                     RRQWEALLEQIGNGAADILVGTQMMAKGHDFPRLTLVGVLNADASLFAADYRAPERLF
                     QQLMQVGGRAGRGALPGEVLIQTQYPEHPLYQCLAAHDFSRYAALQLAERRQARFPPF
                     AANALLCANAAEVATALDWLKAARAAAEPLAVAHGVHLFDPVPMRMVRLARRERAQLL
                     VEAATRGALQGFLTAWSRLLWSQRPPRDLRWHLDVDPAEV"
     gene            complement(20610..21053)
                     /locus_tag="PKX01_03195"
     CDS             complement(20610..21053)
                     /locus_tag="PKX01_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ75157.1"
                     /translation="MPLTAARIAQLQGMPLFGGLTPRTLEVLLRASRIVDAQAGHVFC
                     EEHAPGDTFFILEAGQVEVSKGGPRGRRVLRQLSAGDCFGEMALMDLSPRSATVRALC
                     ASRALEIPAEALQALYECDVEQFALLMMNMGREVSRRLRSAGTLE"
     gene            21272..21562
                     /locus_tag="PKX01_03200"
     CDS             21272..21562
                     /locus_tag="PKX01_03200"
                     /inference="COORDINATES: protein motif:HMM:NF022160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HMZ75158.1"
                     /translation="MRAILLVCFLLALAAAQPVPAADLDLNLRMPGLSLSIGERDRDG
                     RYWDGRQWRDERWWGENCHRYEGHKDFRGHCERRGPGVGRDCPPGQAKKGRC"
     gene            complement(21573..22661)
                     /locus_tag="PKX01_03205"
     CDS             complement(21573..22661)
                     /locus_tag="PKX01_03205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HMZ75159.1"
                     /translation="MRESDLKDRALGALMGAFIGDALAVGPHWYYDLDALQRDYGPWI
                     TDYTAPRPDRYHAGLKPGEPSQAGILLAITLRSLVERGGYDEADFCRCLDDEFFPQLD
                     GTPMQGPGGYTSQSIREAWRRRVQDGQPWGRCGGPADTTEAAERIIAIAVRYALEPAE
                     LAAAVTRNTLLTQTDGTVVAMTVAFGAILGLLVEGHPLDGDVSGTLMARVKAGTLPFH
                     TVTQGALQAPAPGAAEAPVAGRFPSPDALLGPAFIARAARDPDIRIEPAWKVSLVYGM
                     PCAVYHQFPAAYYLAARFADDFESAVLHAVNGGGQNQARAILTGALAGAQTGLSGIPA
                     RFIEGLADSARLQAQCARLVGQLVTANR"
     gene            complement(22678..23742)
                     /locus_tag="PKX01_03210"
     CDS             complement(22678..23742)
                     /locus_tag="PKX01_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HMZ75160.1"
                     /translation="MARSPKLIPALFRLGRTSLRQSTRLGKAVARRGLDDGQTLAKAA
                     RIAMSGVARPAPPPPPRPAGGRWYEGRWGPGPLAMRHYRLFIPTGATARRPLPLLVLL
                     HGCAQDAATFAASTHAARTARDRHFAVLMPEQAREANPQRCWNWFGTPAQVELEVLIL
                     MSLVERVQALYPRCRGPLFALGISAGGAIATTLALHRPTHFTAVGSHSGAAPGSAVTP
                     LQAAQAMQGRRSPDLNRVCDLLRGSPPPPLILIHGDADGVVAPGNASATADLWRATLS
                     AGTPLGESRGEIRRGTRLPCQRHDWRHNGRPWLRVITIHGLGHAWSGGATGFAFSEPR
                     GPDALKLAWQFFAATMEESS"
     gene            complement(24173..24751)
                     /locus_tag="PKX01_03215"
     CDS             complement(24173..24751)
                     /locus_tag="PKX01_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HMZ75161.1"
                     /translation="MSVSPEQLVAVSKANAEASLKTAADFATTLFTAVERMSSLNLSV
                     ARGAFDDTVAFSKAVMAAKDPKEVVALQLGSLPPAVEKGVAYGRSLSEIGGDTKEELT
                     KLAESEAAVLSKNVNTAIEALFKNAPAGSEPAISAVKTAIANATSAYEGASKAAKEVA
                     AAAQATVDKATAVAVESVAKGTKAAASALKAV"
     gene            complement(24915..25475)
                     /locus_tag="PKX01_03220"
     CDS             complement(24915..25475)
                     /locus_tag="PKX01_03220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ75162.1"
                     /translation="MPTSPCAVPLAHATRLLNHGPTVLVSAAHDGLRNVMAAAWNMPL
                     DFEPPKVAVVIDKNTFTRTLIEASGQFVVAVPTRAQAALTLAIGSRSGRDGDKFVALD
                     LDTLPASRVTAPLPVGCIAWLECRVIPEPHVQQTYDLFLGEVLAAWADPAVFAGGRLQ
                     FDDPATRSIHYVAGGRFLMIGEAFDV"
     gene            25561..26424
                     /locus_tag="PKX01_03225"
     CDS             25561..26424
                     /locus_tag="PKX01_03225"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75163.1"
                     /translation="MSTLTPSPVRPARRWLRGIAWLAGALLGLAVLAVAGIALRQERL
                     LFHPEPLPAGFDFAAILPPGQTFETIPVRVPGATLSAILLRQQSSRGLVFYLHGNSGN
                     LATWLGNAAFYRALGYDLFLLDYRGFGQSSGVIDGEAQLHDDVRAAWQRVAPAYAGRP
                     IVVIGRSLGSGLAVRLARDVRPDLLVLVTPYASLSRLAAEHMPYVPSALLRYPLASDR
                     IIGDVRSPVLIFHGDRDEVIDVAHAHSLLALAAADAELVEVAGASHTDIQTFAAYRDR
                     LAVRLRALGGP"
     gene            complement(26438..29431)
                     /locus_tag="PKX01_03230"
     CDS             complement(26438..29431)
                     /locus_tag="PKX01_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75164.1"
                     /translation="MRLARLSLLFSAVLLLSVGMSGLIALQAMQAFDRVQTVQDHRLE
                     ALHAVEALRRDTDTLTRLVRAYTVTGETRFLSYYHALQGIRAGALPVPDDPAPTTYWD
                     RVIAGETLYLPASSGPGMSQRQRMIELGFDARELSALAAAMDEAETLKAMEQNAIAAT
                     RSAYNPDNGRFNGGHRFDPGDAVLQVYGQDYDRQRARLTVAIANLLQQVDARTTREVG
                     EARDGLRAWIAGAVAGVAVTAVLVVLGLRTTRRELLHPIEDISAVTGQLARGQYDVRL
                     PEAGHIAEIRALRQAVNSMARAVERDIAEREFIQRELGEARTRAELATQAKSMFLANM
                     SHEIRTPLNAVIGMAELMLHSPLAPRQREYADKIHSAGRTLRDIVNDILDFTKIEAGH
                     LELERIPFRLEGAIANALLPVEQSAAEKGVELIFEATANSARVLDRSFLGDPVRVGQI
                     LGNLLSNAVKFTPSGHVSLRVDGQNNGPQGMRVSITVEDTGIGMRPEQIERLFDDFTQ
                     ADGATTRQFGGTGLGLAIVRRLVDAMGGQIRVHSQPKQGSTFQVVLPLGVDTTTAPRP
                     APELPAEFRVLVVDDTPAARLALIDLLHLSGVDEVDSASDRATALECLDYAVESGFRY
                     DLLLLDDALPGNGTRTLFDDLQQHPARTPRHLALLSTGECPDDSLPETAECPIHRCDK
                     PLLPGALARLFDRVLGRSPARSDVPASGEVGALDGMRVLLVEDNATSRDVAVALMGRW
                     GVEVDSAVDGRDALSQLAAHPPEHYGLVFMDLQMPVMDGYEATRRLRAQPIYAGLPVY
                     ALSAHSGRQVTNRCLALGMNGCLDKPYEVADLYRVLHQYYRGEGRAPGTLPSPSDGRP
                     VLEGLDRIPGLDPRCAINDTGISATLYPRLLQKFRDQFADGPSSLRADVAAHAWDRVA
                     PFAHTLKGRAGLLGMGEISAIAGRLEAAAHAGDFVRAERLLIALEAHLRPLVEGLARA
                     LPASAGDGDPIASTSCPEHSVSAGS"
     gene            complement(29462..30112)
                     /locus_tag="PKX01_03235"
     CDS             complement(29462..30112)
                     /locus_tag="PKX01_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75165.1"
                     /translation="MHGERGVALGIVATIMLGLAADRFGGPWGQPLVSVWTWLFLGAL
                     LWRAPALRRPLLGCLLIATTGEIVLSLVLHLYDYRLGNLPLFVPPGHCLLYWLGLYVA
                     PRLGRHLTTAIPWAAVGGVSILAVSGLDGLGPLLLALFLLCLRYGPAPRLYVSMFALS
                     LLMELWGTALGCWTWRSTLPLVDWPVTNPPLAAGAFYCVLDLLCGSLRHPQTCHSA"
     gene            complement(30123..31754)
                     /locus_tag="PKX01_03240"
     CDS             complement(30123..31754)
                     /locus_tag="PKX01_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HMZ75166.1"
                     /translation="MLIANNITMQFGAKPLFENVSIKFGDGNRYGLIGANGAGKSTFM
                     KILAGVLEPSGGNVAIDSGERMAFLRQDQFAYEDVRVLDVVMMGHAEMWACMQEKDAI
                     YANPEATEEDYMKAAELEGHFAEYDGYTAEARAGELLLGVGIPTDKHSGPMREVAPGW
                     KLRVLLAQALFANPDILLLDEPTNNLDINTIRWLENVLNERNSTMIIISHDRHFLNQV
                     CTHMADLDYGRIQVWPGTWDDYIEASTQARERQAAANQKAKERVQELQEFVRRFSANK
                     SKARQATSRAKLIEKIKIDEFKPSSRQYPWIRFEYDEKEKLHRQAVEIENLSFGYDPA
                     KPIIKNFSFTADANDRIAIIGENGIGKTTLLKLLVGEGLHGLKPQHGTLKWAEKAKLG
                     YYAQDHAEDFAQDENLTDWISGYVREGGYEGDDAETLIRGTLGRLLFKGDEVKKSVKV
                     ISGGEQGRMLFGKLMLQRKNVLLLDEPTNHLDMESIESLNSGLADFTGTLFFVSHDRE
                     FVSSIATRILEIRGPGDIVDYRGTYEEYLASQGIE"
     gene            complement(31890..32303)
                     /locus_tag="PKX01_03245"
     CDS             complement(31890..32303)
                     /locus_tag="PKX01_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ75167.1"
                     /translation="MSQDPDYSERRTRQRFVARRWGAVCFWVSIDGGERQALNDLSLE
                     GFSYPLDTPPEQPRRFTFSLHLEGIPDTIRGQAETMNHVLGEGGGQLGCRFLSFDDDG
                     ERRLHEWLTTHVMATATVRISEQEAAEIVRGPSLI"
     gene            32595..33248
                     /locus_tag="PKX01_03250"
     CDS             32595..33248
                     /locus_tag="PKX01_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HMZ75168.1"
                     /translation="MKKQFLAAAVLAAASFGASAATVSFSDSFGLATTDWSHALTLQQ
                     FDTSLGTLNSVTFSYGGEVSTSFRLESLDAAAANVTANAAGNLVFGGPISNTLAVSGS
                     ASRSLSAFDGAIDFGGTSGAIVGPVVGNKSDVLTLLSGLSVYEGLGTYTINVDARGLS
                     NASGAGNLVSIINTQALANITVTYDYTVTPPGRVPEPASMALVGLGLAGLGAIRRRK"
     gene            complement(33348..33614)
                     /locus_tag="PKX01_03255"
     CDS             complement(33348..33614)
                     /locus_tag="PKX01_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75169.1"
                     /translation="MKKLILSIAVSTFAAGLASSALAADATCAEQAAAKKLAGAAKNS
                     FIKKCEGGGATAPSACEGQAKEKKLHGAAKSSFVKKCEADAAAK"
     gene            complement(33719..34654)
                     /gene="hprK"
                     /locus_tag="PKX01_03260"
     CDS             complement(33719..34654)
                     /gene="hprK"
                     /locus_tag="PKX01_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HMZ75170.1"
                     /translation="MRQTTVARLFDDNLERLRLRHICGSLETRISVPEDRIWPADMVG
                     HLNLIHPERLQILGSSELMWARRQKREKVVHHVKDILSYRPPALIVADDEDIPSMIRT
                     LCANADVALFATPLPSASIVDQLRGYLSRQLAERIALHGVLMDVLGVGVFITGDSGAG
                     KSELALELISRGHGLVADDIVEFSRIAPNLLVGRCPELLQDVIEVRGLGILNVRTIFG
                     ETACRKKMKLKLVVHLQRRQASQDEPLRLTLEGEEQNILGVPIRRVVLPVAAGRNLAV
                     LLEAAVRSTILLLRGINSTQEFVERQRRAMEDDGY"
     gene            complement(34623..35108)
                     /gene="ptsN"
                     /locus_tag="PKX01_03265"
     CDS             complement(34623..35108)
                     /gene="ptsN"
                     /locus_tag="PKX01_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HMZ75171.1"
                     /translation="MNLIAQLLPPANVVVGLEASSKKRAFEQAGLLFENNHGIGRSTV
                     FDSLFAREKLGSTGLGQGIAIPHGRIKGLKDALGAFMRLSEPVQFDAPDGRPVNLLFV
                     LLVPEQATEHHLQLLSELAQMFSDRAFRHQLATAPDAAAVHELFAQWGGDAADHGRPA
                     V"
     gene            complement(35257..35580)
                     /gene="raiA"
                     /locus_tag="PKX01_03270"
     CDS             complement(35257..35580)
                     /gene="raiA"
                     /locus_tag="PKX01_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMZ75172.1"
                     /translation="MNLNITGHHVEVTPAIREYVTSKLDRVIRHFDNVTSVGVILSVE
                     KLRQKAEVTVHVRGKDIHVESDDADMYAAIDALADKLDRQVLKYKAKTSDHGHDSLKH
                     HVAES"
     gene            complement(35597..37039)
                     /locus_tag="PKX01_03275"
     CDS             complement(35597..37039)
                     /locus_tag="PKX01_03275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMZ75173.1"
                     /translation="MKPSLQLKLSQHLALTPQLQQSIKLLQLSTLELNQEIEKILLEN
                     PMLERDEPEGDLGSSRVEPPGPMAPAGSEAAQEREAPAEPDREGDYDSSHEAGEWLAA
                     GSGGARDDDDESDFQEFQAAVPSLRDHLDAQIALTPLTDRDRALVRFVIEALDDDGYL
                     SQDLEDLIPLLPPELNFDLDDLQIALRQVQSLDPPGIGACDVSQCLRLQLEPRPPSPG
                     RTLALEIVAEHLELLAARDFVKLKRALRCTDDELRDAHALILSLNPRPGAQFSQADTR
                     YVVPDVVVRKIRSTWMVSLNPDAMPRLRINQLYAQILRDNRGASGSLSSHLQEARWLI
                     KNVQQRFDTIQRVSQAIVDRQRQFFDYGDVAMRPLTLREIAEQLDLHESTVSRVTTQK
                     YMATPRGIFEFKYFFGSHVSTDTGGAASSTAIRALIRQLVGAEDKKKPLSDAKIAELL
                     GQQGIVVARRTIAKYRESLDIPPVSLRKTI"
     gene            complement(37054..37776)
                     /gene="lptB"
                     /locus_tag="PKX01_03280"
     CDS             complement(37054..37776)
                     /gene="lptB"
                     /locus_tag="PKX01_03280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMZ75174.1"
                     /translation="MSLLKVSNLRKKYKARTVVHDVSFDVGSGEVVGLLGPNGAGKTT
                     CFYMIVGLVAADGGDIVLDDEVLTHRPIHQRARLGLSYLPQEMSVFRKLTVAENIQAV
                     LELQDMPKARIAERLDELLGELGIDHLRDNTSISLSGGERRRCEIARALATDPRLILL
                     DEPFAGVDPIAVIDIQKIIRFLKEKGIGVLITDHNVRETLGICDRAFIINAGEVLASG
                     RPDDIVRNEQVRQVYLGEHFRL"
     gene            complement(37763..38212)
                     /gene="recX"
                     /locus_tag="PKX01_03285"
     CDS             complement(37763..38212)
                     /gene="recX"
                     /locus_tag="PKX01_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HMZ75175.1"
                     /translation="MIAPLHARALRCLAQREHSRAELARKLDGLGSADEVEAELDRLA
                     DLGLLSDARFAEAYVRAKAARFGSSRLKAELARRGVAADLIGSALHDACGGSEPERAR
                     TVLRRKFDTPPADAREWARQARFLQGRGFSTEIIRSVLKESPGDEPA"
     gene            complement(38238..39269)
                     /gene="recA"
                     /locus_tag="PKX01_03290"
     CDS             complement(38238..39269)
                     /gene="recA"
                     /locus_tag="PKX01_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMZ75176.1"
                     /translation="MDDNKAKALAAALSQIEKQFGKGSIMRLGDGEVESDILTVSTGS
                     LGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGTAAFIDAEHALDVT
                     YAQKLGVNIGELLISQPDTGEQALEIADMLVRSGGVDVLVIDSVAALTPKAEIEGEMG
                     DQLPGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRMDIRRVGTIKKNDEVIGSETKVKVVKNKVAPPFKEALFDILYGEGTSREGEIID
                     LGVTHKFIDKSGAWYAYQGDKIGQGKDNAREFLKANPALAREIENKVRVAVGLPELPG
                     VAPAAAAAA"
     gene            complement(39421..39909)
                     /locus_tag="PKX01_03295"
     CDS             complement(39421..39909)
                     /locus_tag="PKX01_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HMZ75177.1"
                     /translation="MMDAALDALSQRVGAFLAARGWRLVTAESCTGGWVAETVTATAG
                     SSAWFDCGFVTYSNDAKCALLGVSPATLARHGAVSEATAAEMARGALDHSDGDVSLSI
                     SGIAGPGGGTPDKPVGTVCFAWARPGATVQTATVQFDGDRETIRRQAVIHALEELLRR
                     AT"
     gene            complement(39906..40385)
                     /locus_tag="PKX01_03300"
     CDS             complement(39906..40385)
                     /locus_tag="PKX01_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HMZ75178.1"
                     /translation="MKPTPRFLVSDPSHFIALGFGSGLAPRAPGTFGTLLGWALFPLV
                     RAPLADVGFGLLLAACFLAGIVVCERTGRALGVADHGAIVWDEIVAIWLVLWLTPPTL
                     AWQVLAVALFRFFDIVKPPPVRWVDERTRGGFGVMLDDLLAAGYALFVLAACVRLVA"
     gene            complement(40382..41350)
                     /gene="thiL"
                     /locus_tag="PKX01_03305"
     CDS             complement(40382..41350)
                     /gene="thiL"
                     /locus_tag="PKX01_03305"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMZ75179.1"
                     /translation="MASEFALIRRHFTRPARHTDLSVGDDAALIRPAAGMQLAISTDM
                     LVAGTHFFADTDPRDLGWKTLAVNVSDLAAMGATPRWVVLAASLPAADEAWVAAFAEG
                     FFACCEAFGVDAIGGDTTRGPLNLCPTVFGEVPVGQAVLRSGARPGDDLWISGTPGRA
                     ALGLAHLRGDVVLADAHLGACLAALQRPQPRVALGLALRGQATAMLDVSDGLFGDLGH
                     ILEASGVGAIVRRDALPLTDLIAACDDGERAWRACTAGGDDYELLFTAPAPRREAIAA
                     LAAPAGVSLHRIGTITADAGTLYVEGADRVRRPALLRGYDHFGEVS"
     gene            complement(41379..41855)
                     /gene="nusB"
                     /locus_tag="PKX01_03310"
     CDS             complement(41379..41855)
                     /gene="nusB"
                     /locus_tag="PKX01_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMZ75180.1"
                     /translation="MSAPNKSPRRRAREFALQGVYQWLLNQSGLSAIESHMASVTGFD
                     KCDRDLFVGLLRGTLGNAEALQQAFTPFIDRSVGELSPVERAILLMGSFELVHRPETP
                     YRVVINEAIELAKGYGGTDGHKFVNGVLDKLAGRVRADEVEAAAIQRRAARQDKAD"
BASE COUNT         6512 a        13246 c        14666 g         7516 t
ORIGIN      
        1 cgtgcaggcc gccggagacc ttgtagctgt tctggttgaa cttgccgccg gcgtggagca
       61 cgcacatgac gatctcggcg gcggagcgcc ggggctcgtg cttgtcgtcc atcttgacgc
      121 cggtggggat gccgcggccg ttgtcggtga cggagatgga gttgtcggcg tggatggtga
      181 tcacgatgtc gtcgcagtgg ccggcgaggg cttcgtcgat ggcgttgtcc accacttcga
      241 agaccatgtg gtgcaggccg gtgccgtcgg aggtgtcgcc gatgtacatg ccaggccgct
      301 tgcgcacggc ctccagccct tcgagctgct ggatgctgga ttcgtcgtag ttgtcggcgg
      361 ggatttgcgg ttcggacatg gtgttctcgg ttctgcgaag gcgcgctgcg cggcagcgtt
      421 actgcgttgg ttcgggacgt aaacgacccg ggatcacgtt cgtggagaaa gcgtcgcgag
      481 cggttcgagg tcgcactgag gcgcggagct gtaccgggcg tacagcgagc accggaattg
      541 cgggatcgga ccgcgcggca gctttatcca cggacgcctt agatgcgcat gggcatcacg
      601 acgtacttga agtcgggatt gtcgcccagc ccgaacaggg cgctggcgtt gttgtcgttg
      661 aagcggatct cgatttcgtt ggcggagagg ttggtgagca cgtcgagcag gtaggtgacg
      721 ttgaagccga tgtcgagggc gtcgccgctg tagtcgactt cgagctcttc ctgggcttct
      781 tcctgttcgg cgttggtgga gacgagcttg aggctgcctt ccgagaggat caggcgcacg
      841 ccgcggaact tctcgttggt gaggatggcg gcgcgttgca gggactggag cagggcaagg
      901 cggctggcct tgacgagctt gggatggtgc tgggggatga cccgctcgta gtcggggaac
      961 ttgccgtcga tgagcttgga gatgagttcg atgtgtccga agcggaagac gatctggttg
     1021 cccaccaggg tgacttcgac ggggtcgtcg gagtcggcga gctggcgggc cagctccacg
     1081 acggtcttgc gcggcaggat cacgtcggtg cgggggtagt cgccttcgat ggccgatgcg
     1141 gcgtaggcga ggcggtggcc gtcggtggcg accatgcgca gggtgccgcc ggcgacgacc
     1201 aggagcaggc cgttgaggta gtagcggatg tcctgctggg ccatggcgta ctgcaccagg
     1261 gcgagctgct tcttgaaggc cttctgggtg gtggagaagt gcacgccctc gccttcgggc
     1321 ggggccaggc ggggatagtc ggcggcgggc agggtctgga gctggaagcg gctcttgccg
     1381 gccttgaggg tgatgcgctt gtcgtcgagg gtgagctgca cctcggcgct gtcgggcagg
     1441 gctcgcagaa tgtcctgcag cttgcgggcg cccacggtga tggccacgtc ctcggcgccg
     1501 agggcggcgg tggtgcgatt gcggatctgg atttcgatgt cggtggcgag cagggtcagg
     1561 gcttcgccct tcttctcgat caggacgttg gagaggatcg gcagggtatg gcgcttttcg
     1621 acgatgccgg aaacggactg cagcggggcg aggagggcat cgcggggtgc ggtgaagagc
     1681 agcatgtgtc gggtctccgg atcgttgtca gcaagcgagt ggcgttgtgg gtgaagtcgg
     1741 ggccgcggtc atccgcgcag gacctgggtg agcacgtgca ggtcgtggtt gagctgggtg
     1801 tcgccgaggc gcagttcggc gatggtgcgg caggcatgca gcacggtggt gtggtcgcgg
     1861 ccgccgaagg cctcgccgat ggcgggcagg ctcatggggg tgaggtcctt ggcgagggcc
     1921 atggccacct ggcgcgggcg ggcgatgacg cgggtgcgct tcttggagtg catgtcggcg
     1981 accttgatct tgtagtagtc ggcgacggtc ttctggatga actcgacggt gagctggcgg
     2041 ttgtgggcgt tgagcagatc cttgagggcg tccttggcca cctccagggt cacgtcgcgc
     2101 ccgtggaagc gggcaaaggc cagcaccttc ttgagggcgc cttccagctc acgtacgttg
     2161 gagcgcaggt tcttggcgat caggaaggcc acgtcctcgt ccaggctgat gcgctcggcc
     2221 tcggccttct tcttgaggat ggcgacgcgc atctcgagct cgggcggctc gatctgcacc
     2281 gtgaggcccc agtcgaagcg ggagatcagg cggtcttcca ggccctgcac gtccttgggg
     2341 taggtgtcgc tggtgatgat gatctgcttc ttggcctcgg tgagggcgtt gaaggcgtgg
     2401 aagaactctt cctgggtgcg gttcttgtta ttgaagaact ggatgtcgtc gatgatcagg
     2461 acgtccaggg agcggtagta gcgcttgaag acgtcgaagg atttctgctg gtaggcgcgc
     2521 accacgtcgg cgtagtagtc ctcggcgtgg acgtagcgga tcaccttgcg cgggtcggcc
     2581 ttgaagacgg tgttgccgag ggcgtgaatc atgtgggtct tgcccaggcc cacgccgccg
     2641 tagatgaaca gggggttgta ggacacgccg gggttctggg ccacctgcat ggcggcggcg
     2701 cgggccaggt cgttggcgcg gccggtgacc agggtgtcga aggtgaaatc cgggttcagg
     2761 cgcgattttt cgtaattggc ggcctcggcg gtgagcggcg tttcggcgcg ggtcgtgtgg
     2821 acggccgggg cgaccggcgt ggcgatgccc acgccggcgg tggccggtgt actgacggat
     2881 tcgatgccgg acgtggccga gcccggaccg ggcggcggag ccgccgggcg gcgcagaccg
     2941 ccggccggtg cgatgccgac ctcgatgacc ggcggcgcgc cgaagtagtc ttcgcccagt
     3001 tcctcgatgc gccgcaggta gcggtcgcgc agccactggc ggtggaagtt ggagggcgcg
     3061 accaggcgca gaccggcggc gtcgccttcg gcgaggtctt cggcacgcag gggcaggatc
     3121 caggtcttga actgctgggc aggcagttcc tgctcgaggc agccgagaca atggttccag
     3181 aaggcagtgc tgggcgggat ggagctggct tcggacacga aattcctgcg gtgcggttcg
     3241 ggtggctgaa ggggtgccac ttgtcgttat ggtcgtccgc gccgtcgcgg tgacggggcg
     3301 tgcggctggt cgtgagggac gtccggccaa accggaattg tacctgcgcg aggccgagtt
     3361 atccacaagc ggaatgcggt gttgggcctt tgatggcggg tctcggcgga gccggctgaa
     3421 gcgggcggct accgggtccg cactgccctt tgtcgggcgc ggcgtcgggc gcgggcgggg
     3481 cggcttcccg tgccctgttc aatatagctt gacaaacaga gggttaacgt atgaaattac
     3541 gcgtttgact caactttccg ggcaagcaaa atgaaacgta cttaccagcc ttccgtcgtc
     3601 cgtcgcaagc gcactcacgg cttcctggtt cgcatggcca cccgtggcgg ccgcaaggtc
     3661 atcgccgcgc gtcgtgccaa gggccgtcat cgtctcggcg tctgaggctt cttccgcggt
     3721 cggcgccatg aacggtgccg cgacggaaga ttccggcggg ccggcatggg gcttcgggcc
     3781 gtcgttgcga ctgcacaaaa cggatgagtt ctcatccgtt tttgcttttc ggcgcgtggt
     3841 ccggggacgc tacttcgccc tccattacgc cccctgccgg acagacgaac cgcgcctcgg
     3901 gctggtggtt gccaagaagc tcgcccgccg cgccgtgctg cgcaatctgg tgaagcgcat
     3961 cgggcgcgac gtgtttcgtc atgcccatgc aagcctgccg cgttacgatg tggtgttacg
     4021 attgtcggct ccgcttgctt ccgtgacgaa acgccagatg cgcgaagaca tgctcggcct
     4081 gtttggtcgc ctgccccgat gaagtggctc gtgctggcgt gcctgcgcac ctatcgctac
     4141 gccatcagtc ccttgctcgg gcgcaactgc cgtttcttcc cgagctgttc cgagtacgcc
     4201 ctcgaggcgg tcgaacgtca cggcgccctg catggcggct ggctgggcct gcgccgggtg
     4261 tgccgctgcc atccgtggag tgccgggggc tacgatcccg tgccctgagg cgcggttggc
     4321 tgtgctccgg atcgtggaac cctcgcctgc tcacgtctcc tcattcccca ttcacctctt
     4381 atccccgacc tgactccgat ggatacccgt cgcctgatcc tcctcctcgt gttctccttt
     4441 tccatggtga tgctgtggga tggctggcag aagcataccc atcccgcgca gcccgcaacg
     4501 gcggcgtcgg ggacggcgcc cgccagcaat ggggtgccca cgcccacggc cgccaccacg
     4561 gtgagcgccc cgggtgtgcc gggcgtgccg cagatcgcct ctccggccgc tgcggcgccg
     4621 cgcatgaaag tccgcaccga cgtgctggtg gccgacgtgt ccgccgaggg cggcgacatc
     4681 gtccggctgg agctcacgcg ccacaaggcc acgggtgacg ccgagcacga ttacgtgctc
     4741 ttcgacgatg gcgagaagca tctgtatgca gcccagagtg gtctgatcgg caacggcctg
     4801 ccgacccaca agacgcgctt cgcgctgccg gcccaggagc gggtgctgaa ggacggtgag
     4861 gacgcggtct cggtacggct ggaggccccg gctacggcgg acggcattcg cgtcagcaag
     4921 ctgatcacct tccatcgagg cagctatgtg gccgaggtga gctacgagat cgccaacggc
     4981 agcggcgctc cgatcgcgcc ccatgcctac ttccagttca tgcgcgacgg caagccggcg
     5041 gaacaggccg gcggcttcgg ggtgcagacc ttcaccggtc cggcgttcta taccgacgcc
     5101 gagaagtacc agaaagtgaa tttccaggac gtgaccgaca agaaggccaa gttcgtcacc
     5161 aaggcgcctg acggctgggt ggcgatggtg cagcactact ttgtggcggg ctgggcgccg
     5221 gctgcaggcg agcgggagtt cttcgccaac gcgctgggcg gcgacctgtt cgcggcaggt
     5281 gtgatcgttc ccgtggcggc catcgcgccg ggacagagcg gcaccgtctc cgtgccgctg
     5341 tacgccggtc cccaggaaca gagccgtctc gaccagttcg ccaagggttt cgatctggtg
     5401 gtggattacg gctggctgac catcatcgcc gcccccctgt tctgggtgct ggagtggttc
     5461 cacaagctca cgggcaactg gggctgggcc atcatcctgg tcaccatcgc cctgaaggcg
     5521 ctgttcttcc ccctgtcggc cgccagctac aagtccatgg ccaagatgaa gaccctgggc
     5581 ccgcgcctgc agcgcatgaa ggagcagttc ggcgacgaca agatgaagct gaatcaggcc
     5641 atgatggacc tgtacaagcg cgagaaggtg aaccccctcg gcggctgcct gcccattctg
     5701 atccagatcc cggtcttcat tgccctgtac tgggtgctgc tgggcgtggt ggaaatgcgc
     5761 ggtgcgccgt ggctgggctg gatccaggat ctgtcggtga aggatccgta ctacatcctg
     5821 ccgctcatca tgggtgccac cagcctcatc cagaccaagc tcaacccggc tccgcccgat
     5881 ccgatccagg ccaaggtcat gatgatgatg ccgatcctgt tcaccttcat gttcctgtgg
     5941 ttcccggccg gtctggtgct gtactgggtg gtcaacaact gcctgtccat tgcccagcag
     6001 tggcagatca accggatgat cgaagccggt ggcaaggccg ccgagtgagc tgatcgccgc
     6061 gattgccacc gcgccggggc gcggcggcat cggtgtggtc cggctgtccg gatccgatct
     6121 ggcgcccctt gcccgcattc tgggcggggg cgacacacag ccccggaccg cccgcttctg
     6181 ccgctttcgc gacgcggcgg gcgggctgat cgacgagggg ctgctcctct acttcccggc
     6241 gccccactcc ttcaccggtg aagacgtcat cgagctgcaa gggcacggcg gcccggtggt
     6301 gatgcagttg ctcctcggcc gctgcctcga gctgggtgcc cgtctggcgg agccgggcga
     6361 attcacccgt cgtgccttcc tcaacggcaa gctcgacctg gcccaggccg agagcgtcgc
     6421 cgatctcatc gaagcgtcca ccaccgcggc ggcccgttcg gcggtgcgct ccctgagtgg
     6481 cgctttctcc agcgaagtgc atgccctgcg ggatcgcctg atcgatctgc gcatgctggt
     6541 ggaggcgacc ctggattttc cggacgagga catcgacttc ctcgtggagg ctcgcgcctt
     6601 tgaacgcctg gccgtgatgg agggcgaact ggccgccatc ctcgaccggg cgaagcaggg
     6661 cagcctgctg cgcaacggcc tgcatgtggt gctggtgggt cagcccaacg tgggcaagtc
     6721 gagcctgctc aaccgcctcg ccggcgaaga gcgcgccatc gtcacggaca tcgccggcac
     6781 cacccgcgac gcgctgcgcg agacgatcca gatcgaaggc atcccgctgc acatcaccga
     6841 tacggcgggt ctgcgtgaga cttcggacga ggtcgagaag atcggcatcg cccgtacgtg
     6901 gcaggagatc gcgcgggccg acgtggtggt acgcctggtg gatgtccgcg ccggactcac
     6961 ggaggcggac gccgcgatcg atgcgcggct gccggccggg gtagagcgca tcacggtgtt
     7021 caacaaggtg gacctgtcgg gacgggcgtc ggagcgcgtg gagcagggtg acatcggcgt
     7081 gagcctgtat ctctcggcga aggccgggga gggcgtcgac ctgctgcgta ccgagctgct
     7141 gcgggtggcg gggtggcatc gtcacggaga agacgtcatc ctcgcccgtg agcgtcatct
     7201 gatcgcactg cgggaggccc gcgcccgggt gagcgcagcc cgcgacgccg gccatgccct
     7261 ggaactcctg gcagaagagc tgcgcctcgc ccaggacgcg atcaatgcca tcaccggcga
     7321 gttcacgccg gacgacctgc tcggggtcat cttctcccgc ttctgcatcg gcaagtgagc
     7381 gcaccggagt tgtaaccgga ttcgagtgca gcggctttca cttacaaatc catacgactg
     7441 attacatccg tcgctgggtt tcgttcgtaa agtagagaca tcgcatcatc gaccttcccc
     7501 aggagagccc atcatgaaaa aactgaccat tggcttggcg attgtcgctg ctgtcagctt
     7561 cagcggctgt gcaaacatga atgcccaaca gcgcaacacg gccatcggtg ccgcggtggg
     7621 cggcgtggcc ggctccattc ttaccggcgg cagctccatc ggcacggtcg gcggcgctgc
     7681 cgtagggggc gtgatcggca atcaggtcaa gcactgaggc gtacctgtcc aatttggctc
     7741 cgtgttgctg cgttcctccc cgcagcgact ttttgacccg gctcaggccg ggttttttat
     7801 tcagcgtctg acgtagcgcc tttggttgag gctcacgcca ggtcgtcccc gcttaaaggt
     7861 cggtgcgcaa gcccaagggc gggcagtgtc gcgtgcgacg cggtacccac gtgcagcggt
     7921 cgattcggca tgcgtatcga ggtgggctga ttgccgtgag cgctcaacgg gggtgaatct
     7981 ccaccggcac ctgaatcccg ggatgtgggc cgacagcttt accgtctggg cctatcccgg
     8041 cccacgtagc ggcgcgtgtt cgaatccctg gccgcggatt ctcatgttgc ggtgcggtgc
     8101 ggtgcggtgc ggtgcggtgc ggctgtttgc cccagtcgtc ccgattgtgg ggcgtctccg
     8161 gcttcggcga caatgggcgc ttcaatgggg gaggcaccat ggcctgggaa ccggagcgat
     8221 atctgcagtt ttccgatgaa cgtctgcgcc cggcggtcga tctgctcggc cgggtgacgc
     8281 tggcctctcc ggcacggatc gtcgatctgg ggtgtggcgc gggcaatgtg acgcgtctgc
     8341 tccaggcgcg ctggccggat gccgaactcc ggggtgtgga caattcgccc gagatgctgg
     8401 cacgtgcccg acaggcgctg ccgtccgcgc agttcgagct tgccgcggtg gaggactggc
     8461 agccggatgc gcccgtcgat ctggtgtttt ccaatgcagc cttgcattgg gtgggcgaac
     8521 atcatcacct gtttccccgg ctgatgggcg ccgtcgccca aggtggggtg ctggccgtgc
     8581 agatgcctgg caacttcgct gcgccgtccc accgattgat ctgcgagctg gccgaatctg
     8641 acgcgtggcg tgagcgggtg ggtcggggca ggatgggagc catcctggag atggacgcct
     8701 atttcagggt gctgtcccca tgggctcaga ctgtgaccgt atgggagacc acgtattggc
     8761 aggcgctgtc gggggctgcg cccgtactcg actggttgcg cggaacgacg ctggtgccct
     8821 atctcgcacc gatgtcggat ctggaacgtg aggcatttct ggctcaactg ggcgcccgcc
     8881 tggcggtggc ctatccgaag gatgaacagg ggcgcacgct gtttccgttc cgacgtgtgt
     8941 tcctggtggc gcaacgatga cccaggccct cttcgaggct gcggccgacc tgatcgcgca
     9001 ggccgatggt ctgatcatcg cggccggggc tggaatgggg gtcgattcgg gattgccgga
     9061 ttttcggggt aatcaggggt tctggcgggc ctatccggcc ttggccgaag cacggattgc
     9121 cttcgaggag atcgcgtgcc cggatgcgtt ccgggatgct ccggaacgtg cctggggatt
     9181 ctacggccat cggctcaatc tgtatcgacg gacaattcct catgccggat ttgctgtgct
     9241 gaagcggtgg gggctgcgtt tgccgcgggg ggccttcgtg tttacgtcga atgtggatgg
     9301 acagttccag cgtgcgggtt ttgattcgaa ccaggtgtgt gaggtgcatg ggtccattca
     9361 tcacctccag tgcgtgcagg gttgcgatgg ccggattctc tccgcagaag ggtggtcgcc
     9421 ggaggtggat gaggtcggct gcaggttgct gtctccgttg ccgcgctgct cggtctgcgg
     9481 cgatctgctc cggccgaatg tgctgatgtt cggagattgg gcgtggttgc cccaacggac
     9541 gcagtcccaa agggttcgtc tcgagcaatg gttggcggtt gtggatcggc ccgtcgtggt
     9601 ggagatcggg gcgggctctc acgtagcgac ggtgcgctgg ttcagtgaat ccatgggtgt
     9661 gcccctcgtg cgtatcaatc ccaccgaacc ggaggtgcca ggccggggcg tggagttgcc
     9721 catggaggga ttgcgcgcgc tcagcgaaat tgatgcggtt ctggcggcgc gcggcgcatt
     9781 gcgctaggca gctgtttcac gtgaaacacg cggaatggat gcgacctatc tgattctggg
     9841 cctgggcgcc ttctttgcgg gctgcgtcga tgcagtggtc ggtggaggcg gcttgatcca
     9901 gattccgcta ctgttcacga cctttccgac ccaggggacg gcgaccctgt tcggtaccaa
     9961 caagttggcc agcgtcgtag gtacgagcag tgcggcgtgg cgctatgccc ggcgaattgc
    10021 cgtcccgtgg aaggtcattg ctgctgccgc attggccgct ggtgcggggt cctggatcgg
    10081 tgcgcgtgcc gtcgcctatt ttccaccggc ggccttgcgt cccgtgatgc tggcgctact
    10141 cattgcggtc gcgctctaca cgttcgtcag gaaggatttt ggggtgaggc cggatacaag
    10201 tgcgtcgccg tctgatcctt ggcgtcgggc agtgttcatc ggcgcgggcg tcggtttcta
    10261 cgacggtttt tttgggcctg ggacagggag ctttctaatc ttcctgttcg tccgcgcatt
    10321 gaactgggac ttccttcgtg catcggtagc ggccaagatc gtcaacatgg tgacgaatgt
    10381 ggcagccatc ggtttctttg tctcccatgg cgaggtgctg tggcgcgtgg cggccgtcat
    10441 ggccttgtgc aatctgtgcg gcgcgcagct gggctccggt ctcgccttgc ggcatggtgc
    10501 gcccttcgtt cgtcgtgctt ttctgctcgt cgcgtgcgta ctgattgttc gatttgcgct
    10561 cgacaccttt cggatctagg agtgtttcac gtgaaacaga gcctccagca cctactgatt
    10621 ccattgggcg acggggtccg gctggaggtg gagcgaaccg ctgaatccgt cgagctacat
    10681 tcacgctggc agactatccg cacggagcac acggctcgct ttggcgagtt gtactgcctc
    10741 gacggggatg caatggtcag cacggccgac gaattcatga tccatgaaag tctcgcgcat
    10801 cacgtcgcgc tcgaacacac atcacctcgt cgcgccctca ttctgggcgg gggtgatggc
    10861 ggcaccgccc gcgagttgct gaagcatcca tgcatcgacg ccatcgtgat tgcagagctg
    10921 gacgagcgcg tcgtcgcccg ggtgcgcgaa gcgcttccga ccgtgtccgc cggtgctctg
    10981 gacaaccccc gcgtgaccct gcgcctcggc gatgcgggtg aaacgcttga ggcgtcccaa
    11041 caatgcggag aacgcttcga cctcattctg ttcgatctca ccgcaagcga cgatcccgcg
    11101 tgcgcccacc tccacgcccc gcccttcctg aacgcgtgtg ccgaccgtct gatgccggga
    11161 ggccgcctgc acgttcagct gggctcgccg ttctatcagc cagaagctgt tgcggcactg
    11221 catcgccggt tgaacggcgt cttcccttat gtccgccctg ccctcatttc ggtacccctc
    11281 tacggcggcc cctggttgct ggccgcagcc agtgtcgaag cgctgccgga aacgtcggaa
    11341 gcgcagctcg cccaacggct ggccgagcgt gctatcccgc cgcttcgcta ctacaatcca
    11401 gccctccacc gggcgagctt cgccctgccg aacgatgtac acgccctgct cacagcctga
    11461 ttcatgccca tctcccccag acgttcatcg cccgcccgcc ccctgcccga agacagtctg
    11521 gcccacgcac tgtgctgtgc cgcagagctg attgcccgtg tcgccggcgg tgctgccctg
    11581 accgatattc tcgcggacct gtgggtgtcg cgcccagcca caccgccagc ggtccggggc
    11641 gcgattcagg atctgactta cggcacgctg cgggattacg gccgacagga cttcatcctg
    11701 ggtcgcctgc tggacaagtc gctgcctgaa cccgttcagt cgctcctgcg ggtcgcgctg
    11761 caccgcctcg atgttcgacc ggagagtgcg catacggtgg tcgatcaagc cgtgaatgcc
    11821 gcggccaccc tcctttcagg gcgcttcaag ggtgtggtga atggtgtgct gcggaacgcg
    11881 ttgcgccagt ccgccacgct ccgctacgcg gccgatgccg atctcgtcgc cgagatgcgg
    11941 catccgccct ggtggattcg ccgtgtgcgg gaccagcatc cccaggactg gcggaacgtg
    12001 ctcgccgcgg ggaacgtaca tcctccgatg gcgttgcggg tcaaccgtcg gcgcagcacc
    12061 ccggaggccg cggcagctga actcgccgag gccggactga gcgcccggac ctggcccggt
    12121 ggcgcccttc tcctggaacg accggtgccg gtcgtccgca ttccaggttt cgcggaaggg
    12181 cgggtgtccg tgcaggacat cggggcccag gcggcggcac cgctactgga cgtggccagc
    12241 ggacagcacg tgctcgacgc ctgcgcggca cccggcggca agacggccca cctgctggaa
    12301 ttggccgacg tggacctgct ggccctggac gccgatccgc aacgggcccg ccgcatcgac
    12361 gagaatctgc agcgcctcgg tctgagcgcc cgtgtcatgg ccgcagattg ccgtgcgatc
    12421 gatcgctggt gggacggccg tcccttcgac cgcatcctgg cggacgtgcc ctgctcggcc
    12481 tccggggtgg tccgccgtca cccggacatc aagtggctgc gccgctccgg tgacatcggc
    12541 cgcttcgccg cccagcaggc cgagattctc gacgccctgt ggcaggtgct cgccccaggt
    12601 ggcaaaatgc tttacgcgac ctgttcggtg ttccgggagg aaaaccagga tcaggtcgcc
    12661 cggttcgcgg cttcgcatgc ggactgtcat cgcctgacca tcgagggcca aaccgatcgg
    12721 cagttcctgc cttgcgccga ccatgacgga tttttctacg ccctgctcca caaggctgcc
    12781 tgagcgtcgc cgcttcctgc accgcctgag cgccgtcgcc ggcggtgtgg tgctgggcgg
    12841 tctggcaatg cccgcgtgga cggccgaggc cgacatccgt tccgcgtccc tggtcctgac
    12901 ggacgacctg tacgtgcttc acgcagacat cgtcgccgag ctcggttcgc tgctggggga
    12961 cgtactgatg cgtggcgtgt ccctgttctt cgtcgtcgag ttccagctca cgtcgtcgcg
    13021 ctggtactgg ctcgatgaag agatcgccaa ccgtacgctc acctaccgcc tgtcctacca
    13081 ggccctcacg cgccagtacc gcctgtcggt gggggcgctg caccagaact tcatgaccct
    13141 ggatgaggcg ctgcgcacca tgctgcgcgt gcgcaactgg ggtgtcctgg accgcgccgc
    13201 cgtgaagccg gggcacagct acaacgcggc catgcgcttc cgcctcgatg tggcggagtt
    13261 gcccaagccc ctgcaggtca gtgcgttcgg ccgtccggac tggaacctgt cctccgattg
    13321 gctgcgctgg acctttgttc cgcaaaggga cgccaagtga gaccgacggc cctggtcgtc
    13381 gccgcggcgg tcgcaggcat cctgctgttc ctgctggcca cggcgagcgc caacacgccc
    13441 ctgttcgccc gcaactacac gttgttactg gggctcaatg gcgccatcac ggtaggcttg
    13501 gtcgcgcttg tgttcgtcca gttgcggagc ctgtggcaag agcaccgggc gcacctgttc
    13561 gggtcccggc tgaagctgcg cctgatggca atcttcgcag ccgtggccgt ggtgcctgga
    13621 gtgctcgtgt acgcggtgtc gcttcagttt gcggtcaaga gcatcgagtc ctggttcaat
    13681 gtgcgcgtcg acgcggccct cgacagcggg atgcagcttg gccgcacgac gctcgacttc
    13741 ctcctcgatc agctcgccga gaagggcggg cagatggcca tcgagcttga gaccggcggt
    13801 cccatacgcg ccacgcgcct gaaccagctg cgggagcagg ccggggtgga cagcgccacc
    13861 gtatttgccg gcaacgggcg cgtgcaggtg acgtccgaga gtcagatggg cgagctcgtg
    13921 cccgactatc cgagcccgac ccagctcaag caggcccgcg acacgcgcgg ctatcgcgcc
    13981 atcgaagggg acgcggcgag cggattgctg ctgcgggtga tcctgccgat tccgtcgcgc
    14041 catctctccg ccgaggcgcc gctgctccag gtgacccagg aggtgccgcg caatttcgcg
    14101 atccacgccg agagcgtgca gaacgtctat cgcgactacc aggagctgtc cctggcccgc
    14161 aatggcctca agcgcatcta taccctgaca ctcacgctga ccctgctgct ggccttgttc
    14221 ggcgcggtgg cggcggcctt cgtgctgacc cgccgcctgg cggcgccgct gctgatcctg
    14281 gctgaaggga ccgaggccgt ggcggccggg gacttcagcc ctcggcgggc cctgccggcg
    14341 cgcgacgaac tgggcgtcct gacccagtcc ttcaaccaga tgacccgcca gctcgaggag
    14401 gcgcgcggcc tggccgagaa gaatcgggcg gccgtcgaga ccagccgcgc ctaccttgaa
    14461 ggcgtgctgg ccaacctgtc cgccggcgtg cttgcgttct ctcccgaggg cctgttgcgg
    14521 gccgccaatc atggcgcgat gaccatcctc aacgatcgcc tggaaggctg cgagcgcacg
    14581 ccgctggagg cctggcctgc gcatccgatg ttccgcgacg cgctgctacg gggctttcat
    14641 ggcagcgatc cggactggca cgagcaactg gaagtgaccc acgcggacgg cagcacccag
    14701 acactgctcc tccatggcac gcgtttgcca gctgacacgg gtggcggctg ggtggtggtc
    14761 ttcgacgaca tcacccagct catcgccgcc cagcgcaacg cggcctgggc cgaggtggcg
    14821 cggcgccttg cccacgagat caagaacccg ctgacaccca tccagctttc tgccgagcgc
    14881 ctgcagttca agctggcgga gcgcctcgac gacgaaggcc gcgagatgct ggagcgctcc
    14941 acccggacca tcgtcaatca ggtggagtcc atgaagaacc tggtgaacgc ctttcgcgat
    15001 tacgcgaagc tgccggcgcc ggtgcttgtt cccctcgatc tcaacgccct ggtacgggaa
    15061 gtgctcgtgc tttacgaagg ttcgcgggtc atgatccgcg ccgaactggc tgccgaactt
    15121 ccaccggtgc tgggcgacgc gacccagatc cgccaggtca tccacaatct gctgcagaac
    15181 gccgaggacg ccctggtgga cgaaccggac cccgaaatcg tgctgttgac caagtccgac
    15241 gcccgccggg tagacttgct gctgcgcgac aacggcaccg gctttccgcc ggatctgctg
    15301 tcccgtgcct tcgagcccta cgtgacgacc aagtcgcggg ggaccggtct tggcttggcg
    15361 atcgtcaaga aaatcatcga tgaacacagt ggcgagatcc gtctcgccaa tctggaatcg
    15421 ggcggcgccg aggtgcgcat ccgcttccgc tcaagcgagt cactggacca tggcaaaaat
    15481 cctgatcgtt gacgacgaag tcggcatccg cgaactcctc tcggagatcc tgcgcgatga
    15541 aggccacgac atcatcctcg ccgaaaacgc cgctgccgcc cgggccgccc gcaatgcagc
    15601 gcgccccgat ctggtgctcc tcgatatctg gatgcccgac acggatggtg tcagcctgct
    15661 caaggaatgg tccggcaatg gccagctcac catgccagtg gtgatgatgt ccggccacgg
    15721 caccatcgac accgcggtgg aagcgacccg catcggcgcg atggagttcc tcgaaaagcc
    15781 gattgccctg caaaagctgc tggcggcggt gaagcgtggc ctcgcgcggg tcaagccgac
    15841 ccacgcgccc agtccgctgg gactggcagc cttctcgcgt tcgcccgccc tgcgcgatct
    15901 gcgcaagcgc ctcgagcagg tggccgagaa gtcccgcatc atcctgctgc gggtcggtcc
    15961 cggcagtctg gccgaattgt gcgcgcgcac cctgcagcgg gccggcacgc cgtggctgga
    16021 tctgtccctg accaccatgc cgctggagct caataccctt gagagcgccc gcaacggcgt
    16081 gctctatatt cccgaactga tctacctgtc gcgtccccag cagaagaatc tggtgttcgc
    16141 catggatcgc ctggaacgtc acggtcagac gctcgtggtg gccaccgagc atcgcccgga
    16201 ggagcttgtc gaacacggct gggacgaaag ggtggtgctg cgcctgttcg aagtttccct
    16261 cgcgccgccg gccttgtccg agatccgcga cgacattccg gagatcgccg gccagatcct
    16321 gctgcattac gtggaggccg gcgaggtccc gtcgaggcgc ctttcgtcgg cagcgctcaa
    16381 tgccttgcgc aatcagaact ggagcggcgg ttacagcgag ctcaaggcag tggtgaagtc
    16441 cctcgccctg ggcacgctgg aagacgagat ttccatggac gacgtggccc atctgctcgt
    16501 caaggcgccg tccacggcct tcgggctgcc cctcgacctg cccctgcgcg aggcccgcga
    16561 aatgttcgaa cgtctctact tcgagcatca tctgcaactg gacggcggca acatgacccg
    16621 gctcgccgaa aagaccggcc tggagcgcac ccacctctac cgcaagctca agcagctggg
    16681 catcagcgta gggcggcgtc acgaggaagc gagttcctga ccggcccgcg acggttgccg
    16741 gcgattgatc cagcccatcg ccgccggacg ggcaaatgac ggataatccc gcctttgccg
    16801 cactggcggc acgctgccga ggatacgacg tgagccgtcc caagaacgat accttcctgc
    16861 gcgccctgct gcgccagccc accgactaca cgcccgtctg gctgatgcgt caggctggcc
    16921 gctatctgcc cgaataccgc gagacccgca agcgtgccgg cagcttcctc gatctctgca
    16981 agaaccccag cctggcctgc gaagtcaccc tgcagcctct cgcccgctac aacctggacg
    17041 cggccatcct cttctccgac atcctcaccg tgccggacgc catgggcctg ggcctgtatt
    17101 tctcagaagg cgaaggcccg cgcttcgagc gcccgctcaa ggaggaatgg gagatccgca
    17161 acctggcagt gcccgatccc aacctggaac tgggctacgt gatggacgca gtctccgaga
    17221 tccgccgcgc cctcgacggc tcggtgccgc tgatcggctt ctccggcagt ccgtggacct
    17281 tggcctgtta catggtggaa gggggtggct cggacgacta ccgcaagatc aagaccctgc
    17341 tctacacccg gccggacctg ctgcatcaca ttctggacat caccgcccag gccgtcaccg
    17401 cctatctcaa tgcgcagatc gaagccggcg cccaggctgt gatgatcttc gattcctggg
    17461 gtggcgtgct ggcccacgac gcctacaaga ccttttcgct ggcctacatg cggcgcatcc
    17521 tcgcgggcct gatccgtgag aaggacggcg aacgcgtgcc cgccatcgtc ttcaccaagg
    17581 gtggcgggtt gtggctggaa gacatcgccg ccagtggcac cgacgcggtc ggactggact
    17641 ggaccgtcga tatcggggca gcccgccggc aggttggcga ccgggtggcg ctgcagggta
    17701 atctggatcc ttcggtgctg ttcggcagcc ctgaagccgt ggcggaggaa gcacgcaggg
    17761 ttctggacgc gttcggcccc catccgggcc acgtgttcaa cctgggccat ggcatttccc
    17821 tgcatacgcc gccggacaat gtctcggtcc tggtgcagac ggtgcacgcc catagccggg
    17881 cgctgcggca gcaggccgag tgagatcccg gaacgtggtc cgaggccggc gttgccggcc
    17941 ttttttgttg agcacgtcgc aggggccggc cttgcagccg aaccgtattt ggcgaattcg
    18001 cacgccgcgg gcgaatgcac ggagatggaa tgacaatggc gtgggtccgg cagcttgccc
    18061 ggagcgggac gcgtcaagca ctttttttgc gcgagcggcc ttgacttgtg cacagccgca
    18121 tgattcccgc cagacagccc tcaccaaaat tgaggtgatc cggcgtatgt ttataaggca
    18181 ttgtatttaa acgattaaaa ttttgcctag gaaatgagca gggtaggatg aagcctgttg
    18241 gcaggcgggt ttggcaggtt ttcacagggg tttcccacaa agttatccac agaatgtgtg
    18301 gacaatggga aataccgcga ccgggcaagc atttcaggga tatttcagaa gcgttgcgtg
    18361 atgaatgcgg cgcaaattgc tgatttatat ggaaataaac aagatttaac aacattgaga
    18421 aattgccgtg catgtcgtcc gagtggcact tcccgtcccg cttccgcaac tatttgatta
    18481 cacatctgaa aatgtgtctg aatcggatgt ggggcggtgt gtgcgggttc ccttcggacc
    18541 cgccgagaaa aacggcgtca tccttgcgat cgacccgccg gatgggatca cgccggagcg
    18601 tctcaaatcc gtcctggcca tccagcgcga cgcgcctgcc ctgcccgcgg actggctggc
    18661 gctggtggac ttcgcggccc gctactacca gcaccccctt ggtgaagtga ttgcccttgc
    18721 cctgccgcct cgcctgcgtc gcgcggatgc cgtcgccggc gatgaagtgg accccttgct
    18781 cgacgtgacg ccggacggcc accttgcctt ggggcgtagc acgcgcgaaa gccgtgccct
    18841 ggcggtggtc cgccggctgg ccgcggaggg cgcggtccgg cgtgctgcat tgcgggcggc
    18901 cgagggagcg gctgtcgccg atgccctgcg ccgggggtgg cttgccgcct ccgccgcaga
    18961 cgaaccggcg cgcatggcca ccgcgcctga gctcacggag gaacagcgcg ccgccgtggc
    19021 gcgtctcgcc gacggattcg ggcgctacca gccctttctg ctggtcggcg tgaccggcag
    19081 tggcaaaacc gaggtgtatc tgcgcctcgt ccgcgaggcg atcacccgcg gcgggcaggc
    19141 gctgatcctg gtgcccgaga tcgcgctcac gccccagctc gaagcccgcg tggccgggcg
    19201 ctttcccgct gcgcgggtgg tgagcctgca ttccggactg ggcgaggcgg cccgctcgca
    19261 gggcttcgtg caggccctgg aaggccgtgc cgacattgtc ctgggcacgc gcctcgccgt
    19321 gttcacgccc ctgccccgcc tcgccctgat cgtcgtggat gaggaacacg atccgtccta
    19381 caagcagcag gaaggcgtgc gctactcggc ccgcgacctc gcggtgtggc gcggccggca
    19441 acgcggcgtg ccggtcgtgc tgggctcggc cacgccttcc ctcgaaagct ggcacgcggc
    19501 cgagcagggg cgctatggcc gtgtggaact gacgcgccgg gcgattgcgg ccaccctgcc
    19561 tgccgtgcgc accctcgacg tgcgccgcga gaagctggat cacgggctgg ccccgcgcct
    19621 gctggaggcg ctcgatgtcc gtctgcagcg gggtgagcag agcctggtgt tcctcaaccg
    19681 gcggggctat gccccggtcc tgtcgtgtcc ttcctgtggc tgggtgagcc gctgcctcca
    19741 ttgctctgcc aacatggtgc tgcacctgac cgaccgccgg ctgcgctgcc atcactgcag
    19801 cttcgagaca ggtctgccgg tggcgtgccc ggactgcggc gacgtggaca tccagccctt
    19861 cgggcgcggt acccagcggg tcgaagaggt actgaccgag cgcttccccg ccgcgcgggt
    19921 ggtgcgcgtg gaccgcgatt cggcgcgtac ccggcgccaa tgggaagctc tgctggaaca
    19981 gatcggcaat ggggctgcgg acattctggt gggcacccag atgatggcga agggccatga
    20041 ctttccgcgc ctgaccctgg tgggcgtcct gaatgccgat gcgtccctgt ttgccgccga
    20101 ttaccgtgcg ccggagcgcc tcttccagca gctcatgcag gtcggtgggc gggcgggccg
    20161 gggtgcactg cccggcgagg tgttgatcca gacgcagtat ccagagcacc cgctctatca
    20221 gtgcctcgct gcccatgact tcagccgcta cgccgcgctg cagctggcgg aacgtcgtca
    20281 ggcccgcttt cccccgtttg ccgccaatgc cctgttgtgt gccaatgcgg ccgaggtggc
    20341 gacggcgctc gactggctca aggccgctcg cgccgctgcc gagcccctgg cggtggccca
    20401 cggcgtgcac ctcttcgacc ccgtaccgat gcgcatggtg cgcctggcac gccgggagcg
    20461 ggcccagctg ctggtggagg ccgccacgcg gggtgcgttg cagggctttc tcacggcgtg
    20521 gtcgcgcctg ctgtggtcac aacggccccc ccgcgaccta cgctggcatc tggatgtgga
    20581 cccggccgaa gtctgagcgc aggcgccgct cactccagcg tacccgcaga gcgtagccgg
    20641 cggctgacct cgcgccccat gttcatcatc aggagggcga actgctcgac gtcgcattcg
    20701 tagagcgcct gcagcgcctc ggcggggatc tccagggcgc gactggcgca gagggcgcgt
    20761 accgtggcgc tgcgcggcga aagatccatc agggccattt ctccaaagca gtcccccgca
    20821 ctgagctggc gcaggacccg ccgcccccgc gggccgccct tgctcacttc gacctgcccg
    20881 gcctcgagga tgaagaaggt gtcaccgggc gcgtgctctt cgcagaatac atgtcccgcc
    20941 tgggcgtcca cgattcgcga agcccgtagc agaacctcca gtgtccgggg ggtgagaccg
    21001 ccgaagaggg gcatcccttg caattgagcg atgcgggcgg cggtgagcgg cacggtggat
    21061 tccttcgggg ggctggccga tgggccggag atatcctgtt tatagcgcgt gcgcccggca
    21121 ttgacacgtc gtcacggagg ccgtgcgccc gcggcatgtt ctgcacactg tgaaaaagta
    21181 cgacgtcgga tgcgaaatag tgcgcagcgt gaccgcatgc tccggtgcta tcgtgagccc
    21241 gtattccaat cgcctttccc ggggcctcgt catgcgtgcc attctgctcg tctgctttct
    21301 gctcgccctc gcggccgccc agcccgtgcc tgccgccgat ctggacctca acctgcgcat
    21361 gcccggcttg agcctttcca tcggagagcg cgaccgggat gggcgctact gggatgggcg
    21421 gcagtggcgg gacgaacggt ggtggggcga gaactgccat cgttacgagg gccacaagga
    21481 cttccgcggc cactgcgagc gtcgcggtcc gggtgtcggt cgtgactgtc cgccgggaca
    21541 ggccaagaag ggccggtgct gagcgtcgcc gctcaacggt tcgccgttac cagctgtccc
    21601 accagccgcg cgcactgcgc ctgcagccgc gccgaatctg ccaggccctc gatgaagcgg
    21661 gcgggaatgc ccgacaggcc cgtctgcgca cctgcgaggg cgcccgtgag gatcgcccgc
    21721 gcctgattct gaccgccgcc attgactgca tgcaggacgg ccgattcgaa gtcgtccgcg
    21781 aagcgggccg ccagatagta ggccgccgga aactggtgat acaccgcgca cggcatgcca
    21841 tacaccagcg aaaccttcca ggctggttcg atacggatgt ccggatcgcg ggccgcgcgc
    21901 gcgatgaaag ccgggcccag cagcgcgtcg ggtgagggaa agcgtccggc caccggtgct
    21961 tcggcggcgc cgggtgcggg cgcctgaagg gccccctggg tgaccgtatg gaagggcaag
    22021 gtgcccgcct tgacccgcgc catcagcgtg cccgacacgt ccccgtccag gggatgtccc
    22081 tccaccagca gtcccaggat ggccccaaag gcgacggtca tcgccaccac agtgccgtcg
    22141 gtctgggtca ggagcgtatt gcgcgtcacg gccgccgcga gctccgcggg ttcgagggcg
    22201 tagcgaactg cgatggcgat gatccgctcg gcggcctccg tggtatcggc cggtccgccg
    22261 cagcgtcccc agggctggcc gtcctgcacg cggcggcgcc aggcctcgcg gatcgactga
    22321 ctggtgtagc cgcccggtcc ctgcatgggg gtcccgtcca gctgggggaa aaactcgtcg
    22381 tccaggcagc ggcagaagtc cgcctcgtcg tagccgcccc gctccaccag cgagcgcagg
    22441 gtgatggcca gcaggatgcc ggcctgcgag ggttcgccag gtttcaggcc ggcgtgatag
    22501 cgatccgggc gcggcgcggt gtaatcggtg atccacgggc cgtaatcgcg ctggagggca
    22561 tccaggtcgt aataccagtg agggccaacc gccagtgcgt cgccaatgaa cgcccccatg
    22621 agcgccccga gggcgcggtc tttgagatcg gattcacgca tgagtggact ttcgtggtca
    22681 ggacgattct tccatcgtag ccgcaaaaaa ctgccacgcc agcttcagcg cgtccggtcc
    22741 gcggggctcc gagaaggcga agccggtggc gccaccgctc caggcgtgac ccagcccgtg
    22801 gatggtgatc acgcgcagcc aggggcgccc attgtggcgc cagtcgtgac gctggcaggg
    22861 caggcgtgta ccgcggcgga tctcgccccg cgactcgcca agcggcgtgc cggcggacag
    22921 tgtggcccgc cacaggtcag cggtggcgga ggcattgccg ggtgcgacga cgccgtcggc
    22981 atcgccatgg atcaggataa gggggggcgg cggagacccc cgcagcaggt cgcagacccg
    23041 gttcagatcg ggactccgcc ggccctgcat tgcctgagcg gcctgcaggg gcgtgactgc
    23101 gctccccggt gcggcgccgg aatggctgcc gacggcggtg aagtgtgtcg gccggtggag
    23161 ggcgagggtc gtggcgatgg cacccccggc agagatgccc agcgcaaaga gggggccccg
    23221 gcaccgcgga tagagcgcct gcacccgctc gacgagactc atcaggatga gcacttccag
    23281 ctccacctga gcaggtgtgc caaaccagtt ccagcagcgc tgcggattgg cttcccgcgc
    23341 ctgctcgggc atcagcaccg caaagtgccg atcgcgcgcc gtccgtgcgg cgtgggtgga
    23401 ggccgcgaag gtggcggcat cctgggcaca gccatgcagc aggaccagca gtggcagggg
    23461 gcggcgggcc gtggcgccag tgggaatgaa cagccggtaa tggcgcatgg ccagcggacc
    23521 cggtccccag cggccttcgt accagcgacc gccggccggg cgcggcggtg gcggtggtgc
    23581 cggtcgggcg accccgctca tggcgatgcg ggccgccttg gccagcgtct ggccgtcatc
    23641 caggccacgt cgggcgaccg ccttgcccag ccgggtcgac tggcgcaggc tggtacgccc
    23701 gagacggaac agggcgggga tcagcttcgg gctgcgggcc atggcgtcgg tgatgctcct
    23761 gtggcaggcg gttcaagggc ctcgggagga ttgtggcggc aaggcaaaga ccgcgtccag
    23821 tgcggcgctg cgtgaagcgg ggggaggatg gggccgcgcc aggacggcgc gcgatccatg
    23881 cgtcggtctg gccgacctgt atccggcgct cgtagaggcc tgtccggcgg cgggagcggg
    23941 tgtcggtgcc gcgcaggggc gacgatgact gcgttttacg ggacggccag cactgtctgt
    24001 gcgggctgcg atacaggcct gcgccgcggg ccgctgacgg gtgagtgagg gtgagttcgt
    24061 cgtctgcaga ggcgttgcgg gcgacctcgg ttgcaggcgt gccaaaggaa aaggccccgc
    24121 agggcctttt ccggtacgac aggcaagcgc gggcgggccg cgccaggccg gatcagacgg
    24181 ccttcagggc ggaggcggca gccttggtgc ccttggccac gctttccacg gccacggcgg
    24241 tcgccttgtc gacggtggcc tgggcggcgg cggccacttc cttggcagcc ttggaggcgc
    24301 cttcgtaggc ggaggtcgcg ttggcgatgg ccgtcttgac cgcggaaatg gcgggttcgg
    24361 aaccggccgg cgcgttcttg aagagggcct cgatggcggt attcacgttc ttggacagaa
    24421 cagccgcttc gctttcggcg agcttggtga gttcttcctt ggtgtcgccg ccgatttcgg
    24481 acaggctgcg gccgtaggcc acgccctttt caacggcggg cggcagggag cccagctgca
    24541 gggcgaccac ttccttcgga tccttggcgg ccatcaccgc cttggagaag gcgacggtgt
    24601 cgtcaaaggc gccgcgggcg acggacaggt tcaggctgga catgcgttcg acagcggtga
    24661 acagggtggt ggcgaaatcg gcagcggtct tcaggctggc ttcggcgttg gccttgctga
    24721 cggcgacgag ttgttcgggg ctcacggaca tgatgaatct cctggaaagg ttgggatcgc
    24781 agaacccgaa tgaattcggt atggctctat gttgcactgc acaaaacaga aacgcaagcg
    24841 atttttgttg cggtgcacaa aatattctcg tcggcaccgt ttcccgctcc gaacccccga
    24901 agcggcgacc tccttcaaac gtcgaaggct tccccgatca tcaagaaacg accgccggcc
    24961 acataatgga tgctacgtgt tgcaggatcg tcaaattgca agcgtccgcc tgcaaaaacg
    25021 gcgggatccg cccaggcggc gagcacttcg ccgaggaaca ggtcgtaggt ctgctggacg
    25081 tggggttcag ggatgacgcg gcactccagc caggcgatgc agcccaccgg cagcggtgcc
    25141 gtcacgcggc tcgctggcag ggtatcgaga tccagggcga caaacttgtc gccgtcccgc
    25201 ccgctgcggc tgccgatggc cagcgtgagc gctgcctggg cccgcgtcgg gacggcgacc
    25261 acgaattgcc ccgacgcttc gatcagggtg cgggtgaagg tgtttttgtc gatgacgact
    25321 gccactttgg gcggttcgaa atcgagaggc atgttccagg cggccgccat gacgttgcga
    25381 aggccgtcgt gggccgccga gaccagcacg gtgggaccgt gattgaggag acgtgtggcg
    25441 tgggcgaggg gaacggcgca gggcgacgtg ggcatcggcg tgagagggtg tgcatgagca
    25501 tacagtgtag cgcggcggcg tcgcgtgcat ccggccggcg ctcacctgcg tagaccgcgc
    25561 atgagcacct tgacgccctc acccgtgcgc ccggcgcgac gctggctgcg tggcattgca
    25621 tggctcgccg gcgcactgct cggccttgcc gtcctcgcgg tggcgggcat cgcgctgcgg
    25681 caggagcgcc tgctcttcca tccggagccg ctccctgcag gcttcgactt cgccgccatc
    25741 ctgccgccgg gccagacatt cgagacgatt ccggtgcgtg tacccggcgc gacgctgtcg
    25801 gccatcctgt tacgccaaca gagttcccgt ggtctggtgt tctacctcca tggcaacagc
    25861 ggcaatctgg ccacctggct tggtaatgcc gcgttctacc gggccctggg ctacgacctc
    25921 ttcctgctgg attaccgggg attcggacaa agcagcggcg tgatcgatgg cgaggcgcaa
    25981 ttacatgacg atgtgcgggc cgcgtggcag cgcgttgcgc cggcttacgc gggccggccc
    26041 atcgtcgtga tcggacggtc actgggcagc ggcctggcgg tacggcttgc ccgcgacgtg
    26101 cggccggatc tgctggtgct ggtgacgcct tacgcgagcc tgtcgcgtct ggcggccgaa
    26161 cacatgccct acgtgccgtc ggcgctgctc cgctatcccc tcgccagcga ccggatcatc
    26221 ggcgacgtgc ggagcccggt gttgatcttc cacggcgacc gggacgaagt gatcgatgtc
    26281 gcgcatgccc acagtttgct agcgctggcg gccgcggacg ccgaactggt tgaggtggcc
    26341 ggcgccagtc acacggacat ccagaccttc gcggcctacc gggaccgcct ggccgtacgg
    26401 cttcgtgcgc tgggcgggcc ttagggcgcc cgccgactca ggatccggcg gacacggagt
    26461 gttccggaca cgacgtcgag gcgatggggt ccccgtcgcc cgcggatgcc ggcagggcgc
    26521 gcgcgaggcc ctcgaccagg ggacgcaggt gcgcctccag ggcgatcagc agacgttctg
    26581 cgcggacgaa atcacccgcg tgggcggcag cctccaggcg ccccgcaatg gccgagatct
    26641 cccccatgcc gagcagcccg gcgcgtccct tgagcgtgtg ggcgaacggt gcgacgcggt
    26701 cccaggcgtg ggccgccacg tcggcccgca ggctgctggg gccatccgcg aactggtccc
    26761 ggaacttctg gagcaggcgc gggtagaggg tcgcgctgat accggtgtcg ttgatcgcac
    26821 agcgtgggtc gagcccggga atgcgatcga gcccttccag gaccgggcgt ccgtcggagg
    26881 gcgagggcag cgtccccggc gcgcgcccct cgccgcggta atactgatgc aggacgcggt
    26941 acaggtccgc cacctcgtaa ggtttgtcga ggcagccgtt catgcccagc gccagacatc
    27001 ggttggtgac ctggcgcccg ctgtgggccg atagggcgta gaccggcagc cctgcataga
    27061 tcggctgggc gcgcaggcgg cgagtggcct cgtagccgtc catcacgggc atctgcaggt
    27121 ccatgaagac cagcccatag tgctcgggcg ggtgggcggc gagctgggac agggcgtcgc
    27181 ggccatccac ggcgctgtcc acttccaccc cccagcggcc catcagggcg acggccacgt
    27241 cccggctggt ggcgttgtcc tccacgagga ggacgcgcat tccgtcgagg gcgcccactt
    27301 cgccggacgc cgggacgtcc gatcttgcag ggctacgtcc gagtacacga tcgaacaggc
    27361 gtgccagcgc gcccggcagc aggggcttgt cgcagcgatg gatcggacat tcggcggttt
    27421 cgggcaggct gtcgtccggg cattcgccgg tggacagcag ggccagatgg cgtggcgtgc
    27481 gtgcgggatg ctgctgcagg tcgtcgaaga gggtgcgcgt gccgtttccc ggcaatgcat
    27541 cgtcgagcag gaggaggtcg tagcggaagc cggattcgac ggcgtagtcc aggcattcca
    27601 gcgccgtggc ccggtcgctg gcggagtcca cctcgtcgac gccgctgaga tggagcaggt
    27661 cgatgagggc gaggcgcgcc gccggcgtgt cgtccaccac cagcacgcgg aattccgccg
    27721 gcagctcggg cgctggtcgc ggcgccgtgg tggtgtcgac gccgaggggc aggacgacct
    27781 ggaaggtgct gccctgcttg ggctggctgt ggacccggat ctggccgccc atggcatcca
    27841 ccaggcgccg gacgatggca agacccaggc cggtgccgcc gaactggcgt gtggtggctc
    27901 cgtcggcctg ggtgaagtcg tcgaacaggc gctcgatctg ctccggacgc atgccgatgc
    27961 ccgtgtcttc cacggtgatg ctgacccgca tgccctgcgg gccgttgttc tgcccatcca
    28021 cgcgcagact gacgtggccc gagggcgtga acttgacggc gttggacagg aggttgccga
    28081 ggatctgccc cacccggacc ggatcgccca gaaaggatcg gtcgaggacc cgggcgctgt
    28141 tggcggtggc ttcgaagatc agctcgacgc ccttttcggc cgcggactgc tctaccggaa
    28201 gcagcgcgtt ggcgatggca ccttccaggc ggaaggggat gcgttcgagc tccagatgcc
    28261 cggcctcgat cttggtgaag tcgaggatgt cgttgacgat gtcccgcagg gtacggccag
    28321 cgctgtggat cttgtcggcg tactcccgct ggcgcggcgc caggggactg tgcagcatca
    28381 gctcggccat gccgatcacg gcattgagcg gcgtgcggat ctcgtggctc atgttggcca
    28441 ggaacatgga cttggcctgg gtcgccagtt cggcacgggt gcgtgcctct cccagctcgc
    28501 gctggatgaa ctcccgctcg gcgatgtccc gttcgaccgc acgggccatg ctgttgaccg
    28561 cctggcgcag ggcgcggatt tccgcgatgt gcccggcttc gggcaggcgc acatcgtatt
    28621 ggccgcgggc aagctgaccg gtgacggcac tgatgtcctc gatgggatgg agcagctcgc
    28681 ggcgggtcgt gcgcaggccc aggaccacca gcacggccgt gacggcgacg cccgccacgg
    28741 cgccggcgat ccaggcgcgc aagccgtcgc gcgcctcgcc cacctcccgg gtggtccgtg
    28801 catccacctg ctgaagcaga ttggcgatgg ccaccgtgag gcgggcccgt tgccggtcgt
    28861 agtcctggcc gtagacctgc aggacggcat caccgggatc gaagcgatgc ccgccgttga
    28921 agcgcccgtt gtccgggttg taggcgctgc gcgtcgccgc gatggcgttc tgctccattg
    28981 ccttgagggt ctccgcctcg tccatggcgg cggccagggc ggacagctcg cgggcgtcga
    29041 agcccagttc gatcatgcgc tggcgctggg acatccctgg accggacgac gcgggcaggt
    29101 aaagcgtttc gcccgcgatg acccgatccc agtaggtggt gggggctgga tcgtccggta
    29161 cgggcagcgc acctgcgcgg attccctgga gggcgtggta atagctcagg aagcgggtct
    29221 cgcccgtgac ggtgtaagcg cgcacgaggc gggtcagggt gtcggtgtcg cggcgcaggg
    29281 cctccacggc atgcagcgct tcgaggcggt ggtcctgcac ggtctggacg cgatcgaacg
    29341 cctgcatggc ctgcagggcg atgaggccgc tcatgccgac ggacagcagc aggacagctg
    29401 aaaacaggag ggaaagccgg gccagtctca tggcgacggc aagcttacac cctcgccgat
    29461 gtcatgcact gtgacaggtt tggggatgac gcagagaacc gcacagcaga tcgagcacgc
    29521 aatagaaggc accggcagcg aggggcgggt tggtgaccgg ccagtccacc agcggcagcg
    29581 tgctgcgcca ggtccagcag cccagtgccg tcccccagag ttccatcagc agtgacaagg
    29641 cgaacatgct cacgtacagg cgtggagcag gtccataacg caggcacagc aggaacaggg
    29701 caagcaggag cgggccgagg ccgtccaggc cgctcaccgc caggatgctg acgcccccca
    29761 ccgcggccca ggggatggct gtcgtgaggt gtcgccccaa gcgcggtgcg acgtacagcc
    29821 ccagccagta gagcaggcag tgtcccggag gaacgaagag gggcaggttg ccgaggcgat
    29881 aatcgtagag gtgcaggacc agggagagca cgatttcccc ggtcgtggcg atcagcaggc
    29941 agcccagcag cggcctgcgt aaggccggcg cgcgccacag gagcgctcca aggaacagcc
    30001 aggtccacac gctgaccagg ggctgacccc agggaccgcc gaaacggtcg gccgccaggc
    30061 ccagcatgat cgtggcgacg atccccagcg cgacgccccg ctccccgtgc atggcggcgg
    30121 gcttactcga tgccctggct ggccaggtat tcctcgtagg tgccgcggta atccacgatg
    30181 tcgccagggc cgcggatttc caggatgcgg gtggcgatgg acgacacgaa ttcccggtcg
    30241 tgggagacga agaacagggt gccggtgaaa tcggcgaggc ccgagttgag ggactcgatg
    30301 gattccatgt ccaggtggtt ggtcggttcg tccagcagca gcacgttctt gcgctggagc
    30361 atcagcttgc cgaagagcat gcgcccctgt tcgccgccgg agatgacctt gaccgacttc
    30421 ttcacctcgt cacccttgaa gagcaggcgt ccgagggtgc cgcggatcag ggtttcggcg
    30481 tcgtcgccct cgtagccgcc ctcgcgcacg tagccgctga tccagtcggt caggttctcg
    30541 tcctgggcga agtcttcggc gtgatcctga gcgtagtagc ccagtttggc cttctcggcc
    30601 cacttgaggg tgccgtgttg gggcttcaag ccgtgcaggc cctcgcccac cagcagcttg
    30661 agcagggtgg tcttgccgat gccgttctcg ccaatgatgg cgatgcggtc gttggcgtca
    30721 gcggtgaagc tgaagttctt gatgatcggc ttggccgggt cgtagccgaa gctgaggttc
    30781 tcgatctcca cggcctggcg gtgcagcttt tccttttcgt cgtactcgaa gcggatccac
    30841 ggatactgac gactggaggg cttgaactcg tcgattttga tcttctcgat gagcttggcg
    30901 cggctggtgg cctggcgcgc cttggatttg ttggcgctga aacggcgcac gaattcctgc
    30961 agttcctgga cccgctcctt ggccttctgg ttggccgcgg cctgacgctc gcgggcctgg
    31021 gtggaggctt cgatgtagtc gtcccaggtg cccggccaca cctggatgcg gccgtagtcc
    31081 aggtccgcca tgtgggtgca cacctggttc aggaagtggc ggtcgtggga gatgatgatc
    31141 atcgtggagt tgcgctcgtt gagcacgttc tccagccagc ggatggtgtt gatgtcgagg
    31201 ttgttggtcg gttcgtcgag cagcaggatg tccgggttgg cgaacagggc ctgggccagc
    31261 agcacgcgca gcttccagcc gggggccacc tcgcgcatcg ggccgctgtg cttgtcggtc
    31321 gggatgccga cccccagcag cagttcgccg gcgcgggcct cggcggtgta gccgtcgtat
    31381 tcggcgaagt ggccttccaa ctcggccgcc ttcatgtagt cctcttcggt ggcctccggg
    31441 ttggcgtaga tggcgtcctt ttcctgcatg caggcccaca tttcggcgtg gcccatcatc
    31501 accacgtcga gcacgcgcac gtcttcgtaa gcgaactggt cctggcgcag gaaggccatg
    31561 cgctcgccgg aatcgatggc gacgttgccg ccgctgggtt ccagcacgcc ggcgaggatc
    31621 ttcatgaagg tggatttgcc cgccccgttg gcgccgatga ggccgtagcg gttgccgtcc
    31681 ccgaacttga tggagacgtt ctcgaacagg ggcttggccc cgaactgcat ggtgatgttg
    31741 ttggcgatga gcacgacggg aattcctcag gcgccgtccg gcgggacggc aaaacgaaaa
    31801 cgccggcgcg cggccggcga agctggtaca aattttaccg atgattcaag cacctgagca
    31861 aacttcccgg tgcctgtcga aggcgttgct cagatcagcg acggtccgcg cacgatctcg
    31921 gccgcttcct gttcggaaat gcgcacggtg gcggtggcca tgacgtgggt ggtgagccat
    31981 tcgtgcagac gccgttcacc atcgtcgtcg aaggacagga agcggcagcc gagctggccg
    32041 ccgccttcgc ccagcacatg gttcatggtc tcggcctggc cccgaatcgt gtccggaatg
    32101 ccttccagat gcaggctgaa ggtaaagcgg cgcggctgct cgggcggcgt gtcgagcgga
    32161 tagctgaaac cttcgaggga cagatcattg agcgcctgcc gctcgccgcc gtcgatggag
    32221 acccagaagc acaccgcgcc ccagcggcgc gcgacgaagc gctggcgggt gcggcgttcg
    32281 ctgtaatcgg ggtcctggct catcgagagc atcctcctgc ggggtagaga cgggtacgtc
    32341 gcaatgctag gacaaatccg cggattcgcc catggggccg ccggtgacgt cttgcttcag
    32401 ggaagcatgc cacggaaatc atggcgaatg ccgtttgcac tgtcggaaat ttttactgtt
    32461 tccatgtgaa acacatttta tagatcataa atacatgatt taaatcataa aacacaactt
    32521 gggcacaccc gttgctgagc accggcaggc actgatctgc ctgtgacata tttaacactc
    32581 aaacgggagt ttccatgaaa aagcaatttc tcgctgctgc agttctcgcg gctgcgtcct
    32641 tcggcgccag cgccgcgacc gtgtccttct ccgactcctt cggcctggcc acgaccgact
    32701 ggtcccacgc cctgacgctc cagcagttcg acacctcgct gggtaccctg aactccgtga
    32761 ccttctccta cggcggcgag gtgtccacct ccttccgcct ggaaagcctg gatgccgccg
    32821 cggccaacgt gaccgccaac gccgccggca acctggtgtt cggtggcccg atctccaaca
    32881 ccctcgccgt gtccggctcc gccagccgct ccctgagcgc ttttgacggc gccatcgact
    32941 tcggcggcac gtccggcgcc atcgttggtc ccgtggtcgg caacaagtcc gacgtgctga
    33001 ccctgctgtc cggcctgtcc gtgtacgaag gtctgggtac ctacaccatc aacgtggacg
    33061 cgcgtggcct gtccaacgcc tccggtgccg gcaacctggt gtccatcatc aacacccagg
    33121 ctctggccaa catcaccgtg acctacgact acaccgtcac gcctccgggc cgtgtgccgg
    33181 aacccgcctc catggcgctg gtgggcctcg gcctggccgg tctgggcgcg atccgtcgcc
    33241 gcaagtaagc acgacgttgt acaggggccg ttccaacgtg acgggaacgg cccctggaat
    33301 gaaaaaggcc accgtgaggt ggcctttttc atgagcagac ggcgctttta cttggccgca
    33361 gcgtcagcct cgcacttctt gacgaagctg gacttggcgg cgccatggag cttcttctcc
    33421 ttggcctggc cttcacaggc ggacggagcc gtggcgccac ccccttcaca tttcttgatg
    33481 aaactgttct tggcggcgcc agcgagcttc ttggcggcag cctgctcggc acaggtggcg
    33541 tcggcagcca gggcgctgga ggcgagaccg gcagcgaagg tggacacggc aatgctcagg
    33601 atcagcttct tcatcttctc actcctaggt gtgggcggga ctgctccact ataaacgggc
    33661 cgttgtgcac gatgttgacc cgacgcacgt gcggattcgt gaaactctgt gacaacgatc
    33721 aataaccgtc gtcttccatg gcgcggcgct ggcgctcgac gaactcctgg gtggaattga
    33781 tgccacgcag gagcaggatc gtggaacgca ctgccgcctc caggagcact gccaggttac
    33841 ggcccgcggc caccggcagc accacgcggc ggatcggcac gcccaggatg ttctgttcct
    33901 cgccctccag cgtcaggcgc agcggctcat cctggctggc ctgacgacgc tgaaggtgta
    33961 cgaccagctt gagcttcatc ttcttgcggc aggccgtctc gccaaagatg gtgcgtacat
    34021 tgagaatgcc caggccccgc acttcgatga cgtcctgaag cagctcgggg caacggccca
    34081 ccagcaggtt gggcgcgatg cgggaaaatt cgacgatgtc gtcagccacc aggccgtggc
    34141 cgcgagagat gagttcgagc gccagttccg atttgccggc cccggagtcg ccggtgatga
    34201 acacgccgac gcccagcacg tccatcaaca cgccatgcag ggcgatccgc tcggcgagct
    34261 gacgtgacag atagccgcgc agctggtcca cgatgctcgc cgatggcagc ggggtggcaa
    34321 acagggccac gtcggcattg gcgcacagcg tgcggatcat gctcgggatg tcctcgtcgt
    34381 cggcgacgat cagcgccggc ggccggtacg agagaatgtc cttgacgtgg tggacgacct
    34441 tttcgcgctt ctggcggcgc gcccacatga gctcggaaga ccccaggatc tgcaggcgtt
    34501 cggggtggat caggttgagg tggccgacca tgtcggccgg ccagatgcgg tcctcgggca
    34561 cgctgatgcg ggtttccagg ctgccacaga tatggcgcag ccggaggcgc tccaggttgt
    34621 cgtcaaacag ccgggcgacc gtggtctgcc gcatcgccgc cccactgggc aaacagttca
    34681 tggacggccg cagcatccgg tgccgtggca agctggtggc ggaaggcccg gtcgctgaac
    34741 atctgggcca gttccgaaag gagctgaaga tgatgttcgg tggcttgctc cggcaccagc
    34801 aggacgaaca ggagattcac cgggcgcccg tccggcgcat cgaactgcac cggctcggac
    34861 aggcgcatga aggcgccgag ggcgtccttg agacccttga tgcgtccatg gggaatggca
    34921 atgccttgcc cgagcccggt cgaccccagt ttttcgcggg cgaaaaggct gtcgaacacc
    34981 gtactgcgcc cgatgccgtg attgttctcg aagagcaggc cggcctgctc gaacgcgcgc
    35041 ttcttgctgc tagcctcgag gccgacgacc acgttggcgg gtggcagaag ttgggcgatg
    35101 aggttcatgg gcgggaagga ccagaccgga acgtgcttcc gatcgcgatc gggatcgggg
    35161 tgggagaaac ccggcggggg gccggaccgt cgtgtctatt gggggcgcat tatagccaca
    35221 acggtctggc tgaaaagtgc ctgaaacttc aggcgttcag gattcagcga cgtgatgctt
    35281 gagggagtcg tgaccgtggt cgctggtctt ggccttgtac ttcagaacct ggcggtcgag
    35341 cttgtcggcc agcgcatcga tggcggcata catgtcggca tcgtcgctct cgacgtggat
    35401 gtccttgccg cgcacatgca cggtgacttc ggccttctga cgcagctttt ccaccgagag
    35461 gatcacgccg acgctggtga cgttgtcgaa atgacggatc acacggtcga gcttggaagt
    35521 gacgtactca cggatcgcgg gagtgacttc gacgtgatgc cccgtgatgt tgaggttcat
    35581 ggttgttacc tttctgtcag atggttttac gcagactgac cggcgggata tcgagggact
    35641 cgcggtactt tgcgatggtc cggcgagcga ccacgatgcc ctgctggccc aacaactcgg
    35701 caatcttggc gtcggacaag ggtttcttct tgtcctcggc tcccaccagc tggcgaatca
    35761 gcgcccgaat cgccgttgac gaggccgccc caccggtgtc ggtggaaacg tgactgccga
    35821 aaaaatattt gaactcgaag ataccgcgcg gcgtggccat gtacttttgg gtggtcaccc
    35881 gcgacaccgt ggattcgtgt agatccagct gctcggcgat ttcccgcagg gtaagcgggc
    35941 gcattgccac atcgccataa tcgaagaact gacgctggcg gtcaacgatg gcttgcgaca
    36001 cgcgctggat cgtgtcgaag cgctgctgta cgttcttgat cagccagcgt gcctcctgca
    36061 gatggctgga cagggagccg ctggcgcccc ggttgtcgcg caggatctgg gcatacagct
    36121 ggttgatgcg caggcgaggc atggcgtccg ggttgaggct caccatccag gtgctgcgga
    36181 tcttgcgcac gaccacatcc ggcaccacgt agcgggtatc ggcctgggag aactgcgcgc
    36241 caggtcgcgg attgagactg aggatcaggg catgggcgtc gcgcagctca tcgtcggtgc
    36301 agcgcagggc gcgcttgagc ttgacgaagt cccgcgcggc gagcagttcg agatgctcgg
    36361 cgacgatctc cagcgccagc gtacggcccg gagacggcgg ccgcggttcg agctgcaggc
    36421 gcaggcactg gctcacgtca caggcgccga tgccgggcgg atcgaggctt tgcacctgtc
    36481 tgagggcgat ctgcagatca tccaggtcga aattgagttc gggcggaagc agagggatca
    36541 ggtcttccag gtcctggctc aggtagccgt cgtcgtcgag ggcctcgatg acgaagcgca
    36601 ccagcgcccg atcccgatcg gtcagcggag tcagggcgat ctgggcgtcc aggtggtcgc
    36661 gcagggaggg tacggcggcc tggaattcct gaaagtcgga ctcgtcgtca tcgtcccgtg
    36721 cgccgccgct gccggcggcc agccattcac cggcctcgtg gctgctgtcg tagtcccctt
    36781 cccggtcggg ttcggccggg gcctcccttt cctgggcggc ctcgctgccg gccggcgcca
    36841 tgggcccggg gggctcgacg cggcttgagc cgagatcccc ttccggctcg tcccgttcga
    36901 gcatgggatt ctcgaggagg atcttttcga tttcctggtt gagctcgagg gtggacagct
    36961 gcagcagctt gatggattgc tgcagctgcg gggtcagcgc gaggtgctgg gaaagcttga
    37021 gctggagact gggcttcatg agtcgggacg ctctcagagc cggaaatgct cgcccagata
    37081 gacctggcgg acctgctcgt tgcggacgat gtcgtccggg cgcccgctgg cgagcacttc
    37141 gccggcgttg atgatgaagg cccggtcgca gatgccgagg gtttcccgga cgttgtggtc
    37201 ggtgatgagg acgccgatcc ccttttcctt gaggaaacgg atgatcttct ggatgtcgat
    37261 gacggcgatg gggtccacgc cggcgaaggg ttcgtcgagg aggatcaggc gcggatcggt
    37321 ggccagggcg cgggcgatct cgcagcggcg acgttcgccg ccggacaggg agatcgacgt
    37381 gttgtcgcgc aggtggtcga tgcccagctc accgaggagt tcgtcgaggc gttcggcgat
    37441 gcgggccttg ggcatgtcct gaagctccag caccgcctgg atgttttcgg cgaccgtgag
    37501 cttgcggaac acgctcattt cttgcggcag gtaggacaga ccgaggcgcg cgcgctggtg
    37561 gatggggcgg tgggtgagta cttcgtcgtc gaggacgatg tccccgccat ctgccgccac
    37621 gaggccgacg atcatgtaga agcaggtcgt cttgcctgcg ccgttgggcc ccagcaggcc
    37681 gaccacttcg ccactgccga cgtcaaagga cacgtcatgg acgacggtgc gcgccttgta
    37741 tttcttgcgc agattgctga ctttaagcag gctcatcgcc gggggattct ttcaggacac
    37801 tacggatgat ttcggtcgaa aaaccgcgac cttgaaggaa acgggcctga cgtgcccatt
    37861 ctcgcgcgtc cgcggggggt gtgtcgaact ttctccgcag caccgtgcgg gccctttcgg
    37921 gttcggagcc accgcaggcg tcgtggaggg cgctgccgat caggtccgct gccacgcccc
    37981 ggcgggccag ttctgccttg aggcgactgc tgccgaagcg ggccgccttg gcgcgcacgt
    38041 aggcttcggc gaagcgggcg tcggagagca ggcccaggtc cgcgaggcgg tcgagttccg
    38101 cttccacctc gtcggcgctg cccagcccat caagcttgcg cgcgagttcg gcgcgggagt
    38161 gctcgcgctg ggccagacag cgcagggccc gggcgtggag gggggcgatc atccgcaatg
    38221 cgcccgactg aacgttctca ggcagctgcg gcggcggcgg gcgccacccc cggcagctcg
    38281 ggcagcccga cggcgacacg gaccttgttc tcgatctcgc gggcaagggc cgggttggcc
    38341 ttgaggaatt cccgggcgtt gtccttgccc tgaccgatct tgtcgccctg ataggcgtac
    38401 caggcgcccg atttgtcgat gaacttgtgg gtgacgccca gatcgatgat ctcgccctcg
    38461 cgggaggtgc cctcgccgta gaggatgtcg aacagggcct ccttgaacgg gggcgcgacc
    38521 ttgttcttga cgaccttgac cttggtttcg gagccgatca cttcgtcgtt cttcttgatg
    38581 gtgccgacac ggcggatgtc catgcgcacg gaggcgtaga acttgagcgc gttgccgccg
    38641 gtggtggtct cggggctgcc gaacatcacg ccgatcttca tgcggatctg gttgatgaag
    38701 atcaccaggg tgttggtgcg cttgatgttg gcggtgagct tgcgcagggc ctggctcatc
    38761 aggcgggcct gcaggccggg cagctggtcg cccatctcgc cttcgatttc ggccttgggc
    38821 gtgagggcgg ccaccgagtc gatgaccagc acgtccacgc cgccggagcg taccagcatg
    38881 tcggcgatct cgagggcctg ttcgccggtg tcgggctggg agatgaggag ctcgccgata
    38941 ttcacgccga gcttctgggc gtaggtgacg tccagcgcgt gttcggcgtc gatgaaggcg
    39001 gcggtgccgc cgagcttctg catctcggca atgacctgca gtgtgagcgt ggtcttgccg
    39061 gaggattccg gaccgtagat ttccaccacg cggccgcggg gcaggccgcc gatgccgagg
    39121 gcgatgtcga ggccgaggga gccggtggag acggtgagga tgtcgctttc cacctcgcca
    39181 tcgccgaggc gcatgatcga gcccttgccg aactgctttt cgatctggga gagcgcggcg
    39241 gcgagcgcct tggccttgtt gtcgtccatc ttggtattcc ttgcgaaagg ttgtgcggat
    39301 tatggcacag agtttgcgtg cgggtggacg cggcgccgga gcggcatcgc gataagtgat
    39361 attttataca gtgatatgcc ggcgtccagc acaatcgtgc ggatcgcccg cccgcggacg
    39421 tcaggtggcg cgccgcagca gctcttcgag ggcgtggatc accgcctggc gacggattgt
    39481 ttcccggtcg ccgtcgaact ggacggtggc ggtctggacg gtggctccgg ggcgcgccca
    39541 ggcgaagcac acggtgccta ccggcttgtc cggcgtgccg ccgcccgggc cggcgatgcc
    39601 ggagatggac aggctcacgt cgccgtcgct gtggtccagc gcaccgcgcg ccatctcggc
    39661 ggcggtggct tcgctcaccg caccgtggcg tgccagggtc gcgggcgaga cgcccagcag
    39721 ggcgcacttg gcatcattgg aataggtgac gaagccgcag tcgaaccagg ccgagctgcc
    39781 cgccgtggcg gtgacggttt cggccaccca gccgccggtg caggattccg cggtgaccag
    39841 gcgccagccg cgggcggcga ggaaggcgcc gacccgctgc gacagggcgt ccagggcggc
    39901 atccatcagg ccaccagccg cacgcaggcg gccaggacga agagcgcgta gcccgctgcc
    39961 agcaggtcgt ccagcatcac cccgaagccg ccgcgggtgc gctcgtccac ccagcggacg
    40021 ggcggcggtt tgacgatatc gaagaagcgg aacagcgcca ccgccagcac ctgccaggcc
    40081 agggtcggtg gcgtcagcca cagcaccagc cagatggcga cgatctcgtc ccacacgatg
    40141 gcgccgtgat cggccacgcc cagggcgcgc ccggtgcgtt cgcacaccac gatgccggcc
    40201 aggaagcagg cggccagcag caggccgaaa cccacgtcgg ccaggggcgc gcggaccagc
    40261 gggaacagcg cccagcccag cagggtgccg aaggtgccag gcgcccgtgg cgccagcccg
    40321 gagccgaaac cgagggcgat gaaatgggac gggtcggaca ccaggaatcg cggcgtcggc
    40381 ttcatgagac ctcaccgaag tgatcgtagc cgcgcagcag tgcgggccgg cggacgcggt
    40441 cggcgccttc gacgtacagc gtgcccgcgt ccgcggtgat cgtgccgatg cggtgcagcg
    40501 acacgccggc cggagcggcc agcgcggcga tggcctcccg ccggggagcg ggcgcggtga
    40561 acagcagttc gtagtcgtcg ccgccggcgg tgcaggcgcg ccaggcccgc tcgccgtcgt
    40621 cacaggcagc gatgaggtcc gtcaggggga gcgcgtcccg ccgcacgatg gcgcccacgc
    40681 cggatgcttc aaggatgtgg ccgagatcgc cgaagagccc gtcggagacg tcgagcatgg
    40741 cggtcgcctg tccgcgcagg gcgaggccga gggcgacccg cggctggggt cgctgcagcg
    40801 cggcgaggca ggcgccgagg tgggcgtccg ccagcaccac gtcgccgcgc aggtgggcga
    40861 ggccgagggc ggcgcggccg ggcgtgccgg agatccacag gtcgtcgccc ggccgggcgc
    40921 cgctgcgcag gacggcttgc ccgacgggca cctcgccgaa gacggtgggg cacaggttga
    40981 gggggccgcg ggtggtgtcg ccgccgatgg cgtccacgcc gaaggcctcg caacaggcga
    41041 agaaaccctc cgcgaaggcg gcgacccagg cctcgtcagc ggccggcagg cttgcggcga
    41101 ggaccaccca gcgtggcgtg gcacccatcg cggcgagatc ggagacgttg accgcgaggg
    41161 tcttccagcc cagatcccgc gggtcggtgt cggcgaagaa gtgggtgccg gccaccagca
    41221 tgtcggtgga gatggccagc tgcatgcccg ccgcggggcg gatcagggcc gcgtcatcgc
    41281 ccaccgacag gtcggtgtgg cgggccggac gggtgaaatg gcgccggatc agggcaaact
    41341 cggaggccat ggcggattcc gcgtcacccg gcagggcgtc agtcggcctt gtcctggcgc
    41401 gcggcgcggc gctggatggc ggcggcttcc acttcgtcgg cgcggacccg gccggcgagc
    41461 ttgtcgagca cgccgttcac gaacttgtgg ccgtccgtgc cgccgtagcc cttggccagt
    41521 tcgatggctt cgttgatgac gacccggtag ggggtttccg ggcgatggac cagttcgaag
    41581 ctgcccatca ggaggatcgc ccgttccacc ggggaaagtt cgccaacgct gcggtcgatg
    41641 aagggcgtga aggcctgctg gagcgcctcg gcgttgccca gggtgccgcg caggaggccg
    41701 acgaagaggt cccggtcgca cttgtcgaag ccagtgacgc tggccatgtg gctttcgatg
    41761 gcgctcaggc cggactggtt gagcagccac tgatacacgc cctgcagggc gaattcgcgg
    41821 gcacggcggc ggggcgactt gttgggcgcg ctcatgccag ggccttcagc agtcgggcca
    41881 tttccacggc ggcctgggcg cagtcggcgc ccttgacctg catgcgggcg agggcctggt
//
LOCUS       DAOQPA010000013        41853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_371_length_41853_cov_17.945595, whole genome shotgun sequence.
ACCESSION   DAOQPA010000013 DAOQPA010000000
VERSION     DAOQPA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 41853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41853
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_371_length_41853_cov_17.945595"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PKX01_03315"
     CDS             complement(<1..137)
                     /locus_tag="PKX01_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ75181.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKRYGGQFVLAGNIIVRQRGTE
                     Y"
     gene            complement(159..470)
                     /gene="rplU"
                     /locus_tag="PKX01_03320"
     CDS             complement(159..470)
                     /gene="rplU"
                     /locus_tag="PKX01_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMZ75182.1"
                     /translation="MYAVIKTGGKQYRVVAGEKLKVEQIPADVGSQITLDQVFMVGEG
                     ESVKIGTPVVAGATVTATVLSHGRHDKVKIFKMRRRKHYQKHQGHRQNYTELRIEAIS
                     A"
     gene            complement(557..1360)
                     /locus_tag="PKX01_03325"
     CDS             complement(557..1360)
                     /locus_tag="PKX01_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ75183.1"
                     /translation="MKTASSQQAYRPYPSFSTAQTLLWFIGGVLLMFVLVSAAFAIKL
                     GVEGRLPFVRRTVDAPRADAATPPPTPAARPTGQNFSSGAETILPPEVSHLAKTTAAP
                     DTAPARPTEGPAPSAGTPGAATPASAPVTPAPVTPAAAPAPVAAQAPATRPVDPTGDV
                     TEALHAWVTAWARRDVPTYLEHYAQDFRPAAGLSRSAWQSQRRQRLSRGGELLISLSD
                     VQVQVSGDKATARFVQSYRSDTLTSTEPKTLELIRRDGHWLILSEQTGS"
     gene            complement(1479..1904)
                     /locus_tag="PKX01_03330"
     CDS             complement(1479..1904)
                     /locus_tag="PKX01_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75184.1"
                     /translation="MSRRLSALLIALFALGTGWPAHAELVIVAGPRSAITELTREHAE
                     KLYLGRATTLTDGTPVTLMDLPVGPARDDFYLKLAGKNSTQIRAYWSRLVFTGRAMPP
                     READSIAEARQWLADIPNLIGYLDKGDVTPGMRVLLRIP"
     gene            complement(1912..3099)
                     /locus_tag="PKX01_03335"
     CDS             complement(1912..3099)
                     /locus_tag="PKX01_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75185.1"
                     /translation="MKRIAALLILGASLPVAADEPSRFSFSGFGTVGAVRTSNDVLGF
                     RSSVVQDTGPGRAFDTSVDSVIGVQGSAHLTGKSDITLQVVSHKGPDDAFTPQVTWSF
                     LRTQLLPELSVRAGRMRTPFFMFSDALHVNFITPWVRPPVEVYSLNPFSSVDGVDALY
                     RTEAGPMDIEVQGVFGKSSLGIHGGTVKLRAIRTFNVAASGHGLTLHAGYTTANLDVH
                     WGDQPSQNLLAALNATGFGAIADELNGTGAKASFTSAGFQYDRDHWLLIGEFARRDAS
                     HFISSAQGWYFTAGYRVGSVTPYVTYARQLETAPVTSSTVPIPALQTGLDLFNTSRRN
                     AQRTEGIGVRWDFARNFALKGQFDLIRPGPDGLGIFVSPPAGYLTPSGPIHVLSLSVD
                     VVF"
     gene            complement(3096..5540)
                     /locus_tag="PKX01_03340"
     CDS             complement(3096..5540)
                     /locus_tag="PKX01_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ75186.1"
                     /translation="MLSNLSIRVRLLLASTVVQVVMLTLLLTNSARLMNEATGASLET
                     LISQNAAILNIVTASFVPQGRYAELQDALGELLNDSSEGLVYVRIVDASGHTQVRAGM
                     PDLATLPPPDNPATLTLPAGESRNVINIRRPLLMDRNQVGFLQFGVSVSALTVAKQQI
                     LSQGLFIATAEVLLTLLLLGTIGYLMTRNLGRLLRGSQAIAAGDLSHRIPEKGQDELA
                     QLCKHFNRMALALQSRIHELEATARQLAQSEERYALATRAANDGLWDWDIVTGKTYFA
                     PRFAEILGITPDAFVGDRDTLFKHIHPEDREAFRLRLVAHLKRETAQLNVEHRIRHAD
                     GSYRWILTCGMAVYDPDSGRAIRMAGSISDIHLRRRAEDQLVHDALHDGLTGLANRAL
                     FVEHLQNTLRHADRVTGSLFAVLHVNIERFHVVNDSLGYSAGDDLLRLVGQRIQALAR
                     PGDVVGRVGGDQFALLLNDIADASEAIRFSENLQTRLAESAELNGHSLYPKTRIGVAL
                     GSGGFHNAEDLMRDADNAMHRSRSSSDSTITVFQSTMHQQALRDLQLESQLRIALQEG
                     QFVAHYQPIVHLADGRTAGFEALVRWPEQATRRIGPDVFVPLAESLDLIHELGMHMLD
                     QSCAQLKAWRTLGLADGDLSISVNLSARQLMQVDLADQVVECIERHELPPSALKLEIT
                     EGTLVTRKELATSQLGKLRAKGLKILIDDFGTGYSSLSYLHTIPCDTLKLDGSFIREI
                     ERDTRLRAIVGATIRLSHELGISVVAECIETPAQAQLLRDLGCDYGQGYLFSSAQPGD
                     KAQQWLLNNLPVGAGA"
     gene            complement(5728..7455)
                     /locus_tag="PKX01_03345"
     CDS             complement(5728..7455)
                     /locus_tag="PKX01_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HMZ75187.1"
                     /translation="MTAPTPRGEDNAASNFIRNIVDEHRRTGKWSGRVETRFPPEPNG
                     YLHYGHAKSICLNFGLALSYSGACHLRFDDTNPTKEEQEYVDAIIEAVQWLGFDWGEH
                     LYFASDYFDKMYAMAEYLITVGKAYVDSLSAEEMRAYRGTLSAPGQDSPYRNRSVAEN
                     LELFQRMRAGEFPDGTHILRARIDMASPNINLRDPAIYRIRHATHHRTGDRWCVYPMY
                     TYAHPIEDALEDITHSICTLEFEDQRPFYDWLLDALADGGFFPRPLPQQIEFARLNLT
                     YVVLSKRKLIQLVDEGHVAGWDDPRLPTLVGARRRGFTPEGFRQFAERIGVSKADSWI
                     DMSVLEDCMREHLNEAAERRVAVLDPVKLIITNYPEGQIERCHAPNHPLKPELGKREM
                     PFGRELWIEREDFMETPVKGYHRLYPGNTARLRYGYVVRCTGCEKDADGKVTAVLCEY
                     FPDSKSGTPGADTYKVKGNLHWVAVAHAYAAEVRLYDRLFAHPYPGTRREGDPEDLQR
                     DFLDDINPDSLRVIRAQLEPALRAARPEARFQFERHGYFVADRIDTQEGAPVFNRTVT
                     LRDAWKHGA"
     gene            7763..9787
                     /locus_tag="PKX01_03350"
     CDS             7763..9787
                     /locus_tag="PKX01_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ75188.1"
                     /translation="MNPTVLSIAVLSAILALPVHAGERELDDLSLEELVKLEVASVAR
                     KAQKLSDTPAAVTVLSADDIRRSGARSIPEALREVPGVNVAQIGAARWAVSVRGFNGR
                     FSNKLLVQIDGRSVYSPLFSGVFWEAIDVLLEDVERIEVVRGPGASLWGANAVNGIIN
                     IVTRKATATQGTLASVTVDDRGAPVVAARQGFTLANGAALRVFAKGGEISSTRREDGD
                     AVGDKLTGWLAGFRMDSAVGGRNTWSLQGNAYRRSGGDSAVGLVAEAPSGNAFAPQFD
                     GANLLGRYNWGLAGGEATLQAYLDRQQADVGSYGTGAVTTADLDLQHRLAPVGKHALM
                     WGLGYRAFRAEIESRTPMLWMTPNRRDSHIASAFAQDEITLLPRRLMLTLGGRFEYSS
                     LSGFEPQPTVRLMWTPTTTDSVWAGWSRAARTPSIGETDANLVNRIVPLPPGFGPTSA
                     AIVSQPAADWNRRAERVVAVETGYRRNLGNGSVEAVLFHQRYSGLIGGATDPAGLIVP
                     GIAIGVPPGYLPIQRVLRSNNVSGCSSGLELGMDVPVTAWFRLQASYTLMNTSIDPAS
                     DVALATFGGNLKYSAPNHWASVHGVFALGGGRDVDLMLRRVGRVGEGDIPAYTAVDLR
                     YAWKLNRTFELAVVGQNLFDPLHLEYVSDVFPGVPAYLPRRGFVQGVWRY"
     gene            9798..10343
                     /locus_tag="PKX01_03355"
     CDS             9798..10343
                     /locus_tag="PKX01_03355"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HMZ75189.1"
                     /translation="MTFRILRFLLVLWAALGAGLPAVAVPPGEYQIKAAYILNFARYV
                     EWPAARLPGGAVLRVCLLGRDPFDGALHGIDGRLVQGHEVRVRQADSLEQAVDCHVVF
                     VAESEERRTGLILRTLAGRGVLTVSDIDGFAEAGGGIGLVTEEDRVRFDINQAALTRD
                     TLKASSQLLRLGRNVFGLKGL"
     gene            10345..13152
                     /locus_tag="PKX01_03360"
     CDS             10345..13152
                     /locus_tag="PKX01_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75190.1"
                     /translation="MNPLNALRDLPIGRKLSTVVRLGVLIALGVGFLALFVTEIHNEI
                     RRVNNEGRVLSEIIADGAASPLRFDDPRVADSLLASLRHHERVATALLVRPDGRVFAA
                     YPAGVGAAQARVEALKAAAEPGRLGWGLTHLVVAQGIESDGENLGRLIIEFDLHPVLG
                     QFLAWFGYALFGLGGALLAASLLTRRMQAMIVEPIQRLAEVMREVGRYKRYDLRAPAG
                     YADEVGELIAGFNGMLAEIATRDQELTLHRAQLESEVAVRTRELVAAKEQAEAANRAK
                     SQFLANMSHEIRTPMNGVMGMIGLLRQTSLAARQGHFVDMLDDSARALMEVINDVLDV
                     SKIEAGRLELESLAFSLRDTVDQVASLFATSAQQRGIRLRLKVEQDVPDMVRGDALRV
                     RQVISNLVSNAQKFTEHGSIAIQLRRLPCAHPGRVRIRATVRDTGIGVPRGMESRLFQ
                     SFSQADNSMARRFGGTGLGLSIAKQLVTLMDGDIGYDSAPGEGATFWVEIELGAVTQG
                     DAHAVLDGRRALLAMGDPASREALAEQIVFMGGAVEIAPDQAAVTARLASGAVFQWIV
                     TDAGFDGGKGFALLEMLREAPSAPPRLAVVGTGREEAEQARIAGAAEVLAGPLTGVAI
                     RRALLGQERDPDDTSVVPLTARVLVAEDHPVNREIAVAVLESLGCRVAVAENGREAFE
                     ACRRERFDLVLMDIQMPEMDGRQATVAIRADEDFRCLPRVPVVALTANALREDRDACL
                     AAGMDDYLVKPVSREQLGETLAKWLTTFVPVEPAPSLLASPRPAPSGHVEAPAALDMD
                     VLLGLPGVNRNPSSPMLVRLVDLFATETERHVASVRSSFEAGDWSAMRAVAHKMKSGC
                     LALGAVRLAGCARALDDHIREGGVPAAGEVEALAEAWQACRAVMVARGLLAAGEAERA
                     VPPSPDTPPNSPAPTAPQDHD"
     gene            13145..15280
                     /locus_tag="PKX01_03365"
     CDS             13145..15280
                     /locus_tag="PKX01_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ75191.1"
                     /translation="MTEASRARILIVDDDPLTRLMAGEALREGGYAVQEAEDGQQALE
                     LFSARRPDLVLLDVVMPGLGGFEVCARLRALDAGLLVPIIMLTGLDDSESVQRAFDAG
                     ATDFISKPINWTLLRFRVRYVLRSADVMKELVRNKESLSNAQRVARLGSWEWWPEQGS
                     IQRSEQYFRLFGQTAASFGDDAQALVDHVYLPDRATVAAALDEAARGHGYQLTYRVVW
                     PDGSVRTLHETAEPAPSSDPARGRWVMEGSVQDITQQVEAQRRIRQLAYFDSLTGLPN
                     REFFRESLLAAVSRCRRNETGCAVMVLDVDRFARINDSLGPDRGDQVLQVIAHRLREC
                     MGDRAPVEPAGGGGLPDLKVARLASDEFAILVQEAGSPSMLLGVAEGLLATVRAPIHV
                     PGQEIALSARIGVAMMPDHASDADALLKAAETALNRAKTQAGEQVALFSEEMKVAAFL
                     RFSLESDLRRAIENNELTVVYQPIVDVRRRAIVKAEALIRWKHSLRGMIPPPEFISLA
                     EESGLILPLSRRLMDTVFADVARLEPVAPEGFRASVNLSGVNFMDVQLIPMVNGLLAD
                     RGVPATRLEFELTETVLMRDLDYATTILARLREMGVRVAVDDFGTGYSSLAYLHSLAV
                     DTLKIDRSFVNELDSGQGGVIVEAVIGLAQSLGLDVVAEGVETAAQLGALRQRGCHLI
                     QGYLFARPMPVDDLQRLLRDGADFSSILA"
     gene            complement(15290..16033)
                     /locus_tag="PKX01_03370"
     CDS             complement(15290..16033)
                     /locus_tag="PKX01_03370"
                     /inference="COORDINATES: protein motif:HMM:NF023287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HMZ75192.1"
                     /translation="MNAQNPARDVFASPMNCLEFRRLKLADPHRLSAAALAHQRECAL
                     CQAFARRVDTQEQRIATVLDIPLPDGLADRVLVRVHHGRPRPWRFLALAATVVLSVGL
                     SFNLWHDYPRDDYARFAIDHTLHEPESFTDHRLADPSQFRLALARFGAELEAPVGEVL
                     YMKLCPVPGGTGWHIVIRTEHGQATLLLIPRKAGEGSAQGKPLEAEWKGMTALAQPGG
                     DGYYAIVADSPERAQAIAAMMKERIRWRI"
     gene            complement(16030..16548)
                     /locus_tag="PKX01_03375"
     CDS             complement(16030..16548)
                     /locus_tag="PKX01_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ75193.1"
                     /translation="MIFSFTRKREFEQVVRAYSADLYRFAYWLCRDRFVAEDLVQESF
                     ARAWSNWDSLRDKSAPKPWLLTILRNEHARLYERKQFDYVDEQPEELAIAADHDPVAL
                     HELEDLIGRLPLSLREPFLLQTLGGFSCGEIAAQLDTTEGAVMVRLTRARQGLRQLLD
                     GAAAPRHTGSAS"
     gene            complement(16609..17910)
                     /locus_tag="PKX01_03380"
     CDS             complement(16609..17910)
                     /locus_tag="PKX01_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75194.1"
                     /translation="MSEAAAPAFTLLCVDDEANILSALRRLFRPHGYTVLTAGGGEEG
                     LEILAREPVDLIISDMRMPVMDGARFLGEARKRHPDTVRLLLTGYADMESTIEAINAG
                     QISRYISKPWNDQDVLLTVREALERKQLEKEKARLEALTRSQNDELKSLNASLEQKVE
                     ARTAELRIAHDKLKQNFLTSIRVFSNLIELREGAMSGHSRRVADLARKMAIHLGFNAT
                     DAQDVMLAGLLHDVGKIGLRDELLSKPVSHMTGDELGQLRRHPVAGQSALMALENLRK
                     AGTLIRAHHERWDGQGYPDGLSGLAIPIGARLLAVANDFDGLQIGTLTPRKLRQEEAL
                     AFIQQNRGKRYCPQSVDALAAVLGSTESAPAVGNIVSTAALKPGMVLARDLVTPEGVM
                     LLAADFVLDDGLIRQLRELEASEGRRLAIPIRAERKTTASM"
     gene            complement(17907..19718)
                     /locus_tag="PKX01_03385"
     CDS             complement(17907..19718)
                     /locus_tag="PKX01_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ75195.1"
                     /translation="MNPRPYALPVSLFPLADSLPSGLVLHRSDRILFANRAMSRLTGY
                     TADELAQMDFWAFFPKAAQGKVQEWGRMRLQGEHPPARYETLIVIKSGEERWVELTLS
                     CEAIDGARTVLCSYLDITDRKRAEAAQAQSRQLLAQIIDGDPVPTLVINARHIVTHWN
                     QACEQITGIRAADIVGTRKHWTAFYDHERPIMADLIVSGEIETITRHYGQKKLRRSPV
                     VASAYEAEDFFPHFGENGRWLYFTAAPLRNAAGATIGAIETLQDITERRKAEDELRQM
                     HTSLEAKVAQRTVELQDAKATLERDIARRETAEAELRARYSELKDLNSRLSDTQQQLV
                     QSEKMASIGQLAAGVAHEINNPIGYVNSNIRSLKSYIAQLLGVVDAYQAREAALPEPD
                     RAALAAARKAADFDFLREDVTQLLAESEEGTERVRKIVQDLRDFSRTEATQDWQSVDL
                     RRGLDSTLNIASNEIKYRAEVVRDYGEMPLVECLPSQLNQVFMNLFVNAAQAMPEGHR
                     GLITVRTGTEPTREAVWIEVADNGSGMPADVMARIFDPFFTTKPVGKGTGLGLSLSYG
                     IIQKHHGHIGVTSTPGAGTTFRIVLPVHRALTDGATP"
     gene            complement(19723..20574)
                     /locus_tag="PKX01_03390"
     CDS             complement(19723..20574)
                     /locus_tag="PKX01_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ75196.1"
                     /translation="MNPMTAAPTLTDTLARIQRLPVMSAVAMEVLQSLNGADLDVDAL
                     ARRIAQDQAIAARVLRVANSPFYGMAGRVASIHDAIMVLGLSAVRSLVLAAAVVTALP
                     AGHCPGFDERRFWHHVIGTAAAAEAMGKALRRSPGHLFLAGLIHDIGRLALVTLYPAD
                     YAEVLRRAGERDAQLIDVELEIFGFDHAEAGAALARRWNFPDEIADALACHHAPERSQ
                     PGGLAGVLHYADAIAQALDLDGETDSQIPRLHAATVDALGLGRDDLHGVLAGTRARFD
                     SYCLLLG"
     gene            complement(20571..20768)
                     /locus_tag="PKX01_03395"
     CDS             complement(20571..20768)
                     /locus_tag="PKX01_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75197.1"
                     /translation="MSEATEHVDTPPDGAAEEAERAAELERRREAIRARVMHLFRHQA
                     DDPGAQALLHAVLAFRQEQLK"
     gene            complement(20765..22300)
                     /locus_tag="PKX01_03400"
     CDS             complement(20765..22300)
                     /locus_tag="PKX01_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ75198.1"
                     /translation="MDSLAIPPALHYQPRYDCRDGALVGAKLYVARPQRSDNVVLTAL
                     DDACLARLHSACRQVADWNAHHGLDLILTLGATLEVLATPTFRQALNGLILDARLDAH
                     SLELNIITRTGEIPDEQAAALHHLKRCGVRFAISAASPANARLAWLRRLPVDCLEVPA
                     ALVQDVTADPEGVAIIRALVTRAHKMGQRVSAKGIADRHTAGVLVASDCDFVQGPLLG
                     GPMTAEAFGALLAAGERLNPELIRGPRPDRTLLLVDDEENILNALRRLLRRDGYTLLT
                     ASSGQQALDLLATHPVDVIVSDQRMPGMTGVEFLRKAKDMVPDTVRMVLSGYTELQSV
                     TDAINEGSIYKFLTKPWDDAMLRANIDEAFHRKALVDENLRLDAELHRANAELARINE
                     RLAALLAERERQLDVDESALHLTQAALAALPAPVLGTDPGGMIAFANAAADKLFPAHA
                     PLIGLGVDEALPPDLRDLALRGEGATDYRDGARHFHVVAHPLHAVGTCRGTLLSFTDR
                     SGA"
     gene            22522..23532
                     /locus_tag="PKX01_03405"
     CDS             22522..23532
                     /locus_tag="PKX01_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HMZ75199.1"
                     /translation="MSPGSPATMRAMVLHQPRTPLQLERLPVPAPPPGTVRLKVEACG
                     VCRTDLHLMEGELPDPVWPMIPGHEIVGRVDVVGAGVSGFAPGQRVGVPWLGWTCGTC
                     GFCRSGRENLCAQARFTGYQIPGGYAEYTVADARYCFPLPDGGEAAALAPLLCAGLIG
                     YRALRMADDAQKVGLYGFGAAAHLVAQVLRHQQRPFYAFTRPGDIESQAFARQLGATW
                     VGDAATPPSERLDAALLFAPAGALVPAALRHVDKGGCVICAGIHMSDIPSFPYADLWG
                     ERTIRSVANLTRRDGLEFLALAPQVPVRAEITPFPLARANEALDALRRGALQGAAVLT
                     MD"
     gene            23600..24004
                     /gene="crcB"
                     /locus_tag="PKX01_03410"
     CDS             23600..24004
                     /gene="crcB"
                     /locus_tag="PKX01_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMZ75200.1"
                     /translation="MPDHSHSFPTLALVAIGVGAALGAWLRWWLGLRLNPVLAQMPLG
                     TLAANLLGGYLVGVAVAFFALRPDIPPEARLLVVTGFLGGLTTFSSFSAEVVHLISTA
                     RYAWAAGAAAVHLFGSLTMTALGMWTVHAVRG"
     gene            24032..28123
                     /gene="hrpA"
                     /locus_tag="PKX01_03415"
     CDS             24032..28123
                     /gene="hrpA"
                     /locus_tag="PKX01_03415"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HMZ75201.1"
                     /translation="MAAPHDRPDAVRQDPYSLLAHCLTADRPALRRDLRRLPRRPRTL
                     EDLPEALRLRIADSAARRIAREQARPRPDFPDELPVNQRRDEIAAAIRDHQVVIVCGE
                     TGSGKTTQLPKICLELGRGVAGLIGHTQPRRIAARATASRIAQELRTELGSAVGYKIR
                     FTDRIGPQSYIKLMTDGILLAETQGDPLLSAYDTLIIDEAHERSLNIDFLLGYLRQLL
                     AKRPDLKLIVTSATLDAERFARHFGTPERPAPVIEVSGRLYPIELRYRPVERDAPKPL
                     RPGEAPRPEPRGPDRDLYDALVDAVDEAQRSGPGDVLVFLPGEREIREAAEALRKAHH
                     AAGTEILPLFARQSAADQARVFAPSKGRRVVLATNVAETSLTVPGIRYVVDTGLARVK
                     RYAHRNKVEQLQIEKIAQSAARQRAGRCGRVSDGICFRLYDEDDFNKRPAHTDPEILR
                     SSLAGVILRMKALKLGEVADFPFIDAPLPRMVADGYQLLAELGAVEEGGADEAGSARM
                     ALTPIGRELARLPLDPKIGRMILAARDRGCLAEVLVIAAALSTQDPRERPPERQAAAD
                     QAHARFRGPEHEQKSEFLWFWNLWKAWDEVLRHESASKQKAWCKQHFLSWLRLREWRD
                     VHGQLHALCAEHGWKENTEPAAYEALHKALLAGLLGHVGCKIEDATGPAAGSYLGARG
                     IKFWPHPGSSLAKKAGKWIMVAELVDTSRLFGRCLARMEPEWLEEVGAHLLKRQISEP
                     HWSKSHGAVRAWERATLYGLTVYARRGVSYRDIDPALCRELFIREGLVQGEIAEGPAR
                     GMAFLAHNRRLVAEIERLEHKSRRPDVLVDEALIHAFYDAKLPADVVDLASFEAWRKD
                     AERKAPKVLQLTRDQLMRHDAEGITTDRFPAHLDVLGQKLKLTYLHAPGEADDGVTLT
                     VPLAMLNQVPAHRCEWLVPGLLEEKVTALLRTVPQKHRHRLQPMADSAAAFMERFEAG
                     EFDLDEPFLKALQGFVEERVSLKLPMESFRPENLNPHCFMHFRVQDEHGRVLGQSRNL
                     AELRSRFRDQVAARFQSARIVPAADVPATVAPVGKAPAAVDATSPVAGGALAGLTGWT
                     FGPLPELLEVKVAGREIVGFPALHDDGASVSLRPYDTPEEAAKVHRGGLARLFALALA
                     AQVKAIEKLPGLRELALQFLPYGTEAELKAQLVAATLERCCLMAPLPADADAFARCCQ
                     DAKPRITLVAQELMRLAGQLIAEHATLGKRLAGLKTFPEVVADITAQAARLMPKDFIV
                     ALPYERLAQVPRYLKGASVRIDKLRNNAARDAQLQADWKRLAQPFEREWLAKARAGVT
                     DPQLEEFRWLLEELRVGLFAQELKTPMPVSVKRLQKIWDSRPR"
     gene            complement(28350..30704)
                     /locus_tag="PKX01_03420"
     CDS             complement(28350..30704)
                     /locus_tag="PKX01_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75202.1"
                     /translation="MDTVNQAPPGVWLRGMLSPAPDVRTVSGDPLRLLGESAAALTDG
                     RVCLAERIHPDDQDVAARLFDPHAPAADGPLHLRLRRADGRIVVTCATCTHHASADGQ
                     HFELHLSDSRDLPRTLDDVATLSVRAMLENTDDYIYFKDRHHVFAAASQTLVSLCEPV
                     GHWTDFLGQTDYDVFPEAYADVYYRLEKQVFAGAQVAHEVQPYLHRDGVTRGWVDNRK
                     YPVLDADGRIIGLFGIARDITDKVLAEAALRESEYFFRESQRAAHIGSYKFDVGSGRW
                     TASEVLDDIFGLAPAPDRQLQDWLDLIHPDDRDGMHRYLLDHVLTQRQPFNREYRILR
                     RCDGSLRWVLGCGQLELDATGHPTHMFGTIQDITARRLLDEELADYRAHLEQRVDERT
                     RELQDAKNAAEAASLAKSAFLANMSHEIRTPLNAITGMAHLIRKEPLSPQQAERLAKL
                     ETAGQHLLEILNAVLELSKIEAGKFALEEQPVRLAQVVADVVALLQWRSAGKGVVLRS
                     EVGPLPANLLGDATRLQQALLNYTANAVKFTERGEIVLRAGIVEDGPADVLVRFDVED
                     TGIGITADALPRLFNAFEQADNSTTRRYGGTGLGLAITRKLAQIMGGEAGADSQPGRG
                     SRFWFTARLRKGPTAAEAARPDPAATLLALSTRHAGARVLLAEDEPINQEITQLLLEE
                     ARLVADIAENGEAAVAKARDNDYAVILMDMQMPRLDGLDATRQIRQLPRHARTPILAL
                     TANAFAEDEARCRQAGMNAFIAKPIHPEALYGEVLRVLEGEYAS"
     gene            30930..31367
                     /locus_tag="PKX01_03425"
     CDS             30930..31367
                     /locus_tag="PKX01_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75203.1"
                     /translation="MYDILTLEPEDDVVMRAIKAVIAGRATIGGPEGDYRLLTPGDTW
                     VEGDGGTGVEVLVIVGHGNANKLSGCGKWTLFREHFDGRVDWNHKTEVYLAACSTAGE
                     GGSAFVHGHFAGGVRAAFPTRVRVWASTTAVRGRDLTGDWVQL"
     gene            31615..31848
                     /locus_tag="PKX01_03430"
     CDS             31615..31848
                     /locus_tag="PKX01_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75204.1"
                     /translation="MRLNLSLSILALAVVAGCSSPSHASTHERALVTAELVASRPACA
                     GFIRQLRETVATDQALAETYAAMRAAGCLRPDV"
     gene            31934..32608
                     /locus_tag="PKX01_03435"
     CDS             31934..32608
                     /locus_tag="PKX01_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HMZ75205.1"
                     /translation="MKALHRSAPVRAVTARPRLIFSTICGVLALVALPDTWAHQPVSR
                     GILAWNIGCWLYLALSLTMMFRSTHERMQRRALDQDAGQRTVLALVLLAAVMSLGAIV
                     AELAVAKDLTGPSRLLHIAFAAVTILSSWLLTQVMFAQHYAHDFYLAEARQGSGGLAF
                     PGGHAPDYGDFLYFACVIGTSGQTADVSFTTRAMRRTGLFHCVLSYFFNATVLALTIN
                     IASGLV"
     gene            32747..33448
                     /locus_tag="PKX01_03440"
                     /pseudo
     CDS             32747..33448
                     /locus_tag="PKX01_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
     gene            33547..34635
                     /locus_tag="PKX01_03445"
     CDS             33547..34635
                     /locus_tag="PKX01_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ75206.1"
                     /translation="MSTPVAARHYPPGGDAHGRAVTLKAAVGQLVVAPVEGAALHVPA
                     ARLQASPRGFNHSQWALAWAGADGDHLLIVDDAAVGPLRNGLPGLFATGDQARQRAGL
                     HRVFAYGVLILACLALVVGGVWGFDGLVDRVVAHIPASVEAQIGEAVLARTRQDGPFI
                     ETGPAAEAVQAIGRRLTRPGETLQFHLVQSPQVNAFAAPGGVVVVYSALLRQAGSADE
                     VAGVLAHEIAHVELRHSLRQLVRSAGLQVIAAALFGDYAGLGQLGAQLGALKFSRDAE
                     READARGLQRLADAHIDPQGLLRFFETLADTEGMAATMPAVLSTHPATAERIDALKRV
                     LAAHPAEAVTPLAVSWPAVHAALDKTAR"
     gene            complement(34887..35801)
                     /locus_tag="PKX01_03450"
     CDS             complement(34887..35801)
                     /locus_tag="PKX01_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ75207.1"
                     /translation="MSVPLAYLSIVLIWSTTPLAIQWSTQGAGYALAVFARMLIGVSV
                     AALLMALWRIPLPFHARARRAYLSGGIGLFGAMALTYWGARFVNSGLVSVLFGLSPLI
                     AGGLASVWLGEDALTRPKLAGTLLGAAGLAVIFVHGGSVGGEHAVAGLAALLAAVCVY
                     SASLVSLKRIGDDSPPLATTVGTLAVSLPGFGLMWWLGDGHLPAELPARTGAAIAYLG
                     VFGSVIGFALYYYVIQHMDASKVALITLITPVMALLLGHGLNGEALGPRVWIGTGLIL
                     AGLGVYQDLPGRFRLRPAPDTDGARRQP"
     gene            complement(35815..35943)
                     /locus_tag="PKX01_03455"
     CDS             complement(35815..35943)
                     /locus_tag="PKX01_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75208.1"
                     /translation="MTCIRLSFRRPAGFARISRHAPALRPDVRVPVPPSPPPSSPC"
     gene            36263..36739
                     /locus_tag="PKX01_03460"
     CDS             36263..36739
                     /locus_tag="PKX01_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ75209.1"
                     /translation="MDKARFVEIVRDTPLVSVDLVVLRDHREVLLGLRTNRPAQGTWF
                     VPGGRVLKNERRADALQRVAQRELGIADLHALAPEFLGPFEHFYPDCFAGDNGVSTHY
                     VVLAHLIHVPAGFEIPGQDDQHEALRWWPLDEADAEPAVHRFTRDYLPLLRAHAMR"
     assembly_gap    37250..37349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            37666..38037
                     /locus_tag="PKX01_03465"
     CDS             37666..38037
                     /locus_tag="PKX01_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75210.1"
                     /translation="MNNVSESTRVSPKQVQKDIESYHALGSMADYHANNAENSREAAD
                     TAYQALRAAEQQAVTTQAAASAAADALVVARRNFHGVILGVKNEARALYGPSSDQVAA
                     LGLKKKSEQSKRARSKKTPAE"
     gene            complement(38264..39151)
                     /locus_tag="PKX01_03470"
     CDS             complement(38264..39151)
                     /locus_tag="PKX01_03470"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HMZ75211.1"
                     /translation="MLDYKLLEALDAVAASGSFEGAARRLHLTQSAISQRIRQLEERS
                     GTVLLVRDTPVRATAAGQKLLAHVRQVRLLEAELQETLETAASDWLTLRIGVNADSLA
                     LGLIAAIAPSLQAARILLDCVVADEAQTLDQLRSGEVLGCIGTQPQQIAGCGVIPLGV
                     MQYVPVATPEFVARCFPAGLGADALADAPAAVYGQQDSIHRRWLREAWGLQPGDYPCH
                     VIPDSTALFGAVRAGLAYGLVPLPQARGALERGLLCELAAGEPIGMPLFWHHWNRQTA
                     PARVLADAVALFAGKLADR"
     gene            39248..40480
                     /gene="lysA"
                     /locus_tag="PKX01_03475"
     CDS             39248..40480
                     /gene="lysA"
                     /locus_tag="PKX01_03475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMZ75212.1"
                     /translation="MKPVNGPQLVQIAREYGTPCWIYDAAIIRERIGELRQFDVIRFA
                     QKACSNTHILRLMREQGVTVDSVSLGEIERALAAGFSVGGVHSDIVLTADLLDRATLA
                     RVVELKVPVNAGSPQMLEQLGQASPGHPVWLRINPGFGHGHSQKTNTGGETSKHGIWH
                     EQLDEAYALIERYGLNLVGLHMHIGSGVDYRHLAEVCDAMVAQVKASGRDLQAISAGG
                     GLSIPYTVEGERIDTAHYFSLWDKARCEVEAHLGHPVTLEIEPGRFLVAESAKLIAEV
                     RAKKDAGSNHFVLCDAGFNDLMRPVMYGSHHEISVLKPDGAARTTDQRPTVVAGPLCE
                     SGDVFTQEEGGTVIPRTLPTAEIGDWLVFHDVGAYGASMSSNYNTRPLIPEVLIDGGE
                     IRLIRRRQTVAELLALEN"
     gene            40480..41058
                     /locus_tag="PKX01_03480"
     CDS             40480..41058
                     /locus_tag="PKX01_03480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ75213.1"
                     /translation="MDAAHAYEHHAHSFLAGRDASGIGARVIRDWCAMLPPGATLIEL
                     ACGGGYPVTRVLREAGLRVWAVDSSPTLLATFAQRFPEIPVQCAKVQESDFFGRTFDA
                     AIAVGLIFLLPEAEQVALIARVAQILEPGGRFLFMAPVERGEWQDLNTGLGCRSLGQA
                     AYEGHLSDAGFRVVATFVDKGANHYYDAQRVR"
     gene            complement(41319..>41853)
                     /locus_tag="PKX01_03485"
     CDS             complement(41319..>41853)
                     /locus_tag="PKX01_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ75214.1"
                     /translation="PVDAQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVAAGHK
                     AAFDATVIPGVAYTHPEVAWVGYTEAQAKAEGKKVDTAKFPWAASGRAIANGADYGFT
                     KLIFDAETHRVIGGTIVGPSAGDMIGEVCLAIEMGADAVDIGKTIHPHPTLGETVGMA
                     AEVAHGSCTDVPPARKK"
BASE COUNT         6284 a        13462 c        15064 g         6943 t
ORIGIN      
        1 tggtattcgg tgccgcgctg gcgaacgatg atgttgccgg cgaggacgaa ctggccaccg
       61 tagcgcttga cgcccaggcg tttgctttct gagtcgcgac cgttacggga actgccgcca
      121 gcctttttgt gtgccatgtc ggtttctcct tgggccgctt aggccgaaat cgcttcgatg
      181 cgaagctcgg tgtagttctg gcgatgcccc tggtgcttct ggtagtgctt gcggcggcgc
      241 atcttgaaga tcttgacctt gtcgtggcgg ccgtgggaca gcacggtggc cgtcacggtg
      301 gcgccggcca ccacgggggt gccgatcttg accgactcgc cttcgccgac catgaagacc
      361 tggtcgagag tgatttgcga gcccacgtca gccggtatct gttctacttt gagtttttcg
      421 ccggcgacga cacgatactg cttgccgccg gtttttatga ccgcgtacat ggatgtactc
      481 cgattaaagg gtttgcagga aacgcagcag tatagggcaa ggaaacgctt gcgtcaaacc
      541 ccgatttgtg cgtccttcag ctgccggtct gttcgctgag gatcagccag tggccgtccc
      601 ggcggatcaa ctccagggtc ttgggttcgg tcgaggtgag ggtgtccgag cggtagcttt
      661 ggacaaagcg ggccgtggcc ttgtcgccgc tgacctgcac ctggacgtcg gacaggctga
      721 tgagcaactc gccgccacgg gacagacgct ggcgacgctg gctctgccag gcggagcggc
      781 tcaggcctgc cgcgggtctg aagtcctgcg catagtgttc cagatacgtg gggacgtcgc
      841 gacgtgccca ggcggtcacc caggcgtgca gggcctcggt gacgtcgccg gtgggatcca
      901 ccgggcgcgt tgccggagcc tgtgctgcga cgggggcggg tgccgcggca ggcgtcaccg
      961 gggcaggcgt caccggggca gatgccggcg tcgcggcgcc aggcgttccg gcgcttggtg
     1021 cgggaccttc agtcgggcgc gccggcgcgg tgtcgggggc cgcggtcgtc tttgcgaggt
     1081 gtgagacttc gggcggcagg atcgtctcgg cgccactcga aaagttctgg cctgtgggac
     1141 gcgcggcggg tgtcggcggc ggcgttgcgg catcggcgcg cggagcgtcg accgtccggc
     1201 ggacgaaggg cagccggcct tcgacgccaa gcttgatggc gaaggcggca gacaccagga
     1261 caaacatcag cagaaccccg ccgatgaacc acagcagcgt ctgggccgtg ctgaaactcg
     1321 ggtaagggcg gtaggcctgc tgggaggagg cagtcttcat ggtacggact caatcgttca
     1381 atccggacca ttctaggaaa tcctcgtgca aagcaatgcg gcacgcggcc gctcagcgct
     1441 caagttctac gtatgtttgc aattgggcgt cggaagtgtc acgggatgcg cagcaaaacg
     1501 cgcattcctg gcgtgacgtc gcccttgtcc aggtagccga tgaggttggg gatgtccgcc
     1561 agccattggc gcgcctcggc gatggaatcc gcttcgcggg ggggcatggc gcggcccgtg
     1621 aagacgagcc gggaccagta ggcgcggatc tgggtggagt tcttgccggc cagtttgagg
     1681 tagaaatcgt cccgcgccgg tccgacgggg aggtccatga gggtgacagg cgtgccgtcg
     1741 gtcagggtgg tggcacggcc gaggtaaagt ttttcggcat gctcccgggt gagctccgtg
     1801 atggcgctgc gcgggcctgc gacgatcacc agttctgcgt gggcaggcca tcccgtcccc
     1861 agcgcgaaca gggcgatgag gagcgctgag agccgtctac tcatgggggg cttaaaaaac
     1921 gacatcgacg gacaggctca gtacgtgaat cggcccgctg ggcgtgaggt aacccgcggg
     1981 aggactgacg aagatgccga ggccgtcggg gccgggccgg atgaggtcga actgcccttt
     2041 caaggcgaag ttgcgggcga aatcccagcg gacgccgatg ccttcggtgc gctgggcgtt
     2101 gcgtcgggag gtgttgaaga gatccagacc ggtctgcagg gcgggaatgg ggaccgtgct
     2161 gctggtcacc ggggcggtct cgagctggcg ggcgtaggtg acataggggg tgacgctgcc
     2221 cacccgatag cctgcagtga aataccagcc ctgcgcgctg ctgatgaaat ggctggcatc
     2281 gcgacgggca aattcgccaa tgagcagcca gtggtcgcga tcgtactgga agccggcgga
     2341 cgtgaagctc gccttcgcgc cggttccgtt gagttcatcg gcgatagcgc cgaagccggt
     2401 ggcattgagg gccgcgagga ggttctggga gggctggtcg ccccagtgca cgtcgaggtt
     2461 ggctgtggtg tagccggcgt gcagggtgag tccgtggccg ctggcggcga cattgaaggt
     2521 gcggatggcg cgcagcttga cggtaccgcc atggatgccc aggctggact tcccgaacac
     2581 accctggacc tcgatgtcca tggggccggc ctcggtgcgg tacaaggcat ccacgccatc
     2641 gacgctggag aaggggttga ggctgtagac ctcgaccggc gggcggaccc acggcgtgat
     2701 gaaattgacg tgcagcgcgt ccgaaaacat gaagaacggc gtccgcatgc gcccggcccg
     2761 gacgctcagc tccggcagga gctgggtacg gaggaagctc caggtcacct ggggcgtgaa
     2821 ggcgtcgtcc gggcctttgt gggagacgac ctgcagggtg atgtcggact tgccggtcag
     2881 gtgggcggat ccctgcacgc cgatcacgct gtcgacggag gtatcaaagg cccgtccggg
     2941 ccctgtgtcc tgcacgacgc tgctgcgaaa gcccagcaca tcattgctcg tccggacggc
     3001 tcccaccgtg ccgaatccgg agaaactgaa acgcgagggt tcgtcggcgg cgaccgggag
     3061 acttgctccg aggatcagaa gggcggcgat gcgcttcatg ccccggcacc gacgggaagg
     3121 ttgttgagca gccattgttg cgccttgtcg cccggctggg ccgaggaaaa caggtagccc
     3181 tgaccgtagt cacaccccag gtcgcgcagc agttgggcct gggcgggcgt ttcgatacat
     3241 tccgcgacga cgctgatccc gagctcgtgg gacaggcgga tggtggcgcc gacaatggcg
     3301 cgcagccgtg tatcccgttc gatctcacgg atgaaggagc cgtcgagctt gagtgtgtcg
     3361 cagggaatgg tgtgtagata gctcagggac gagtagccgg ttccgaagtc gtcgatgagg
     3421 atcttgagac ccttggcacg cagcttgccg agctggctgg tggcgagctc cttgcgtgtg
     3481 accagggtgc cctcggtgat ttccagcttg agggcgctgg gggggagttc gtggcgttca
     3541 atgcactcga cgacctggtc ggcgagatcc acctgcatca gctgacgggc ggaaaggttg
     3601 acgctgatgc tcagatcgcc gtctgcaagg ccgagtgtgc gccaggcctt gagctgggcg
     3661 cagctctggt cgagcatgtg catgcccagt tcgtggatca gatcgaggga ttcggcaagg
     3721 gggacgaaga cgtccgggcc gatccgtcgg gttgcctgtt cgggccagcg gaccagggcc
     3781 tcgaagcctg ctgtgcggcc gtcggccaga tgcacgatgg gctggtagtg ggcgacgaac
     3841 tggccttcct gcagggcgat gcgcaactgg gattccagtt gcaggtcgcg cagggcctgc
     3901 tggtgcatgg tggactggaa gaccgtgatg gtgctgtcgg agctgctgcg cgagcggtgc
     3961 atggcgttgt ccgcatcgcg catcaggtct tcggcattgt ggaagccgcc gctgcccagg
     4021 gccacgccga tgcgggtctt ggggtagagc gaatggccgt tgagttcggc gctctcggcg
     4081 aggcgggtct ggaggttttc cgaaaagcgg atcgcttcgc tggcatcggc gatatcgttg
     4141 aggagcaggg cgaactggtc gccgccgacc cgtccgacca cgtcgccggg ccgcgcgagg
     4201 gcctggatgc gctgacccac gaggcgcagc aggtcgtcac ctgccgagta gccgaggctg
     4261 tcgttgacca cgtggaagcg ttcgatattg acgtgcagca ccgcgaacag gctgcccgtg
     4321 acccggtcgg catggcgcag ggtgttctgc aggtgttcca caaagagggc gcggttggcg
     4381 agaccggtga gaccgtcgtg cagggcgtca tgcacgagct ggtcttcggc ccggcggcgc
     4441 aggtggatgt cgctgatgga gccggccatg cggatggcgc ggccgctgtc ggggtcgtag
     4501 accgccatgc cgcaggtgag gatccagcga tagctgccgt cggcgtggcg gatgcggtgt
     4561 tcgacgttga gctgggccgt ctcgcgcttg aggtgggcca ccaggcgcag gcggaaggcc
     4621 tcccggtctt ccggatggat gtgtttgaag agggtgtccc ggtcgcccac gaaggcgtcg
     4681 ggcgtgatgc cgaggatctc ggcaaagcgc ggcgcgaagt aagtcttgcc ggtgacgatg
     4741 tcccagtccc acaggccgtc gttggcggcg cgggtcgcca gggcgtaccg ctcttcgctc
     4801 tgggcgagct ggcgcgcggt ggcttccagc tcatggatgc gcgactgcag ggcgagggcc
     4861 atgcggttga agtgcttgca cagctgggcg agctcgtcct gacccttttc cggaatgcga
     4921 tggctgaggt cgccggccgc gatggcctga ctgccccgca gcaggcggcc caggttgcgg
     4981 gtcatcaggt agccgatggt gcccagcagg agcagggtga ggagcacctc ggcagtggcg
     5041 atgaacagcc cctggctgag gatctgctgc ttggcgacgg tcagcgccga caccgagacg
     5101 ccgaactgga ggaagcccac ctggttacgg tccatcagga gcggccgccg gatgttgatg
     5161 acgttgcgtg attcccccgc cgggagggtc agggtggcgg gattgtcggg ggggggcagg
     5221 gtcgccagat ccggcatgcc ggcgcgcacc tgggtatggc cgctggcatc cacgatgcgt
     5281 acgtagacga ggccttcgct gctgtcattg aggagttcgc cgagggcgtc ctgaagctcg
     5341 gcgtagcgcc cctgggggac gaagctggcg gtgacgatgt tgaggatcgc ggcgttctgg
     5401 gagatcaggg tttccaggct ggcaccggtg gcttcgttca tgagccgcgc gctgttggtg
     5461 agcagcaggg tgagcatcac cacctggacg acggtgctcg ccagcaggag tcggacgcgg
     5521 atcgagaggt tgctcagcat ggacaggttc aggggctcga ccgggtgtgt cgctccatcc
     5581 gggcaccgcg caacggatgg ggagggctgg cggggcaggg tccgacatac atcggcagca
     5641 tcgcccgaag cttgaacgcc gcgccttgcg gcgacgcccg ccgaggcggg caagctgtcg
     5701 ccggaaactt cacggattat ccactgttca tgcgccgtgc ttccatgcgt cgcgcagggt
     5761 caccgtgcga ttgaacaccg gagcgccttc ctgtgtgtcg atgcggtcgg cgacgaagta
     5821 gccgtgccgt tcgaactgga agcgggcttc cggacgtgcg gcgcgcaggg ccggttcgag
     5881 ctgggcgcgg atgacgcgca ggctgtccgg gttgatgtcg tcgaggaagt cccgctgcag
     5941 gtcctcggga tcgccttcgc gacgggtgcc cggatagggg tgggcgaaca gccggtcgta
     6001 gaggcgcact tcggcggcgt aggcgtgtgc caccgcgacc cagtgcaggt tgcccttgac
     6061 cttgtaggtg tcggcgccgg gcgtaccgga cttggaatcc gggaagtact cgcacagcac
     6121 ggcggtcacc ttgccgtcgg cgtccttctc gcagccggtg cagcggacga catagccgta
     6181 acgtagccgt gcggtgttac cgggatagag gcggtggtaa cccttgaccg gggtctccat
     6241 gaagtcctcg cgctcgatcc acagttcgcg gccgaagggc atctcgcgct tgcccagctc
     6301 cggcttgagg gggtggttcg gcgcgtggca gcgctcgatc tggccctcgg ggtagttggt
     6361 gatgatcagt ttgaccgggt cgagcacggc gacgcggcgt tcggccgctt cgttgagatg
     6421 ctcgcgcatg cagtcttcga ggacgctcat gtcgatccag gaatcggcct tggagacgcc
     6481 gatgcgctcg gcgaactggc ggaaaccttc cggcgtgaag ccgcggcggc gggcgccgac
     6541 gagcgtgggc aggcgagggt cgtcccagcc ggccacgtgg ccttcgtcca cgagctggat
     6601 cagcttgcgc ttggacagca ccacgtaggt gaggttgagg cgggcgaatt cgatctgctg
     6661 gggcaggggc cgcgggaaga aaccgccgtc ggcgagggcg tccagcagcc agtcgtagaa
     6721 ggggcgctgg tcttcgaatt cgagggtgca gatggagtgg gtgatgtcct ccagcgcgtc
     6781 ctcgatgggg tgcgcgtagg tgtacatcgg gtacacgcac cagcggtcgc cggtgcggtg
     6841 gtgggtggcg tggcggatgc ggtagatggc cgggtcgcgc aggttgatgt tgggcgaggc
     6901 catgtcgatg cgggcacgca ggatgtgggt gccgtcgggg aactcgccgg cgcgcatgcg
     6961 ctggaagagt tccaggttct cggccacgct gcggtttcga tacgggctgt cctggccggg
     7021 cgcggagagg gtgccccggt aggcgcgcat ctcttcggcg gacagggagt ccacgtaggc
     7081 cttgcccacg gtgatcaggt attcggccat ggcgtacatc ttgtcgaagt agtccgacgc
     7141 gaagtacagg tgctcgcccc agtcgaagcc cagccactgg acggcctcga tgatggcgtc
     7201 cacgtattcc tgctcttcct tggtggggtt ggtgtcgtcg aagcgcaggt ggcaggcgcc
     7261 gctgtagctg agggccaggc cgaagttgag gcagatgctc ttggcgtggc cgtagtgcag
     7321 gtagccgttg ggctcgggcg ggaagcgcgt ttcgacacgc ccgctccatt tgccggtgcg
     7381 acggtgctcg tcaacgatgt tgcggatgaa gttgctggcg gcgttgtctt cgccgcgggg
     7441 ggtcggtgcg gtcatgggct taccttgggt gggcgcggcg atggccgtct ggacgccctt
     7501 attgcattgt aaatgctcga ttgtagccga acgtcgggtt gccggcccgg ttcggtcgcg
     7561 atgcgttccg ccctgaattt cacatttttg tgaggcggga aacagtgtga caaaattggc
     7621 gtgtgtattg acgaattagt taacgttggc ggatttgaga aatattcgag ggtcgtatcg
     7681 accttgcatc gggtgaaatt ccgctcaagt tgtcccgctt tccgtcgtct tgaacgtgtc
     7741 gcccgcactg tcctccttgt ccatgaatcc caccgtcctg agtattgccg ttctgagtgc
     7801 catccttgcc ctgccggtcc atgccggtga acgcgagctc gatgatctga gcctggaaga
     7861 gctggtcaag ctggaagtgg cgtccgtcgc ccgcaaggcc cagaagttgt cggacacgcc
     7921 tgcggccgtg acggtcctgt ccgccgacga tatccgccgc agcggcgccc gcagcatccc
     7981 cgaggccttg cgcgaggtgc ccggcgtcaa tgtggcgcag atcggggcgg cacgctgggc
     8041 ggtgagcgtg cgcggcttca atgggcgctt ctccaacaag ctgctggtgc agatcgacgg
     8101 gcgcagcgtc tacagccccc tgttttccgg cgtgttctgg gaagccatcg acgtgctgct
     8161 cgaggacgtg gagcgcatcg aggtggtccg aggccccggc gcgtccctgt ggggcgccaa
     8221 tgcggtgaac ggcatcatca acatcgtcac ccgcaaggcg acggccaccc agggcacgct
     8281 ggcctccgtg accgtggacg atcgcggtgc gccggttgtc gcggcgcggc aaggtttcac
     8341 cctggcaaac ggcgcggccc tgcgggtctt cgccaagggc ggcgagattt cctcgacgcg
     8401 gcgtgaggac ggcgacgcgg tgggtgacaa gctgacaggc tggctggccg gattccgcat
     8461 ggattcggcc gtggggggcc gcaacacctg gtccctgcag ggcaacgcct accggcgcag
     8521 cggcggtgat tcggccgtgg ggctggtggc ggaagcgccg tccggcaatg ccttcgcccc
     8581 gcagttcgac ggcgccaatc ttttgggacg ttacaactgg gggctggccg gcggcgaggc
     8641 gacgctgcag gcctatctgg atcgacagca ggccgacgtg ggcagctacg gcaccggcgc
     8701 ggtcacgacc gccgatctgg acctgcagca ccggctggca cccgtaggca aacacgccct
     8761 gatgtggggg ctgggttacc gggcgttccg cgccgagatc gaatcccgca cgccgatgct
     8821 gtggatgacg cccaaccgcc gcgacagcca tatcgccagc gcgtttgccc aggacgagat
     8881 tactttgctg ccgcgaaggt tgatgctgac cctggggggg cgtttcgagt acagcagcct
     8941 gtcgggcttc gagcctcaac ccaccgtgcg cctgatgtgg acgccgacca ccaccgacag
     9001 tgtgtgggcg ggctggtcgc gggccgcgcg cacgccgtcc atcggcgaga ccgacgcgaa
     9061 tctggtcaat cgcatcgtgc cgctgccgcc gggattcggc ccgacgtccg cggccatcgt
     9121 ctcccagccg gccgccgact ggaaccgtcg cgcagagcgg gtggttgccg tggagaccgg
     9181 ctatcggcgc aatctgggca acgggagtgt cgaggcggtg ctcttccacc agcgctacag
     9241 cggtctgatt ggcggcgcca cggatccggc ggggctgatc gtgccgggca ttgccatcgg
     9301 cgtgccgcca ggctatctgc cgatccagcg cgtgctgcgc agcaacaacg tgtccggttg
     9361 cagctccggg ctggaactgg gcatggatgt gccggtgaca gcctggttcc gtctgcaggc
     9421 cagctacacc ctcatgaaca cctcgatcga ccccgcatcg gacgtggcgc tggccacctt
     9481 tggcggcaac ctgaagtaca gcgcccccaa tcactgggcg agtgtgcacg gggtgttcgc
     9541 cctgggcggc gggcgcgatg tggacctgat gctgcgccgc gtggggcgcg tgggggaggg
     9601 cgacatcccg gcctacaccg cggtggacct gcgctatgcg tggaagctga accggacgtt
     9661 cgagcttgcc gtggtgggtc agaacctgtt cgaccccctg cacctggagt acgtctccga
     9721 cgtgttcccc ggcgtgccgg cgtatctgcc gcggcggggc tttgtccagg gtgtgtggcg
     9781 ttactgaggc cgcccccgtg acgttccgca tactgcgctt cctgttggtg ctgtgggccg
     9841 ccctcggggc cggcctgccg gcggtcgccg taccgcccgg cgagtaccag atcaaggcgg
     9901 cctacatcct caacttcgcc cgctacgtgg agtggccggc ggcgcgcctg cccggcgggg
     9961 cggtgctgcg cgtctgcctg ctcgggcggg atcctttcga cggggcgctg cacggcatcg
    10021 acggccgcct ggtgcagggt catgaggtgc gggtccgcca ggcggacagt ctggagcagg
    10081 cggtggactg ccacgtggtg ttcgtcgccg agtcggaaga gcgccgcacc ggcctgatcc
    10141 tgcgcaccct ggccgggcgt ggcgtgctga ccgtgagcga tatcgacggc tttgccgagg
    10201 cgggaggcgg aatcggcctg gtgaccgagg aggatcgcgt ccgtttcgac atcaatcagg
    10261 ccgcgctgac ccgcgacacc ctcaaggcca gctcgcagct gctgcgcctg ggacgcaacg
    10321 tcttcgggct gaaggggctg tgagatgaat ccgctgaacg ccctgcggga tctgcccatc
    10381 ggccgcaaac tgagcaccgt cgtacgtctg ggggtgctca tcgcgctggg ggtcgggttc
    10441 ctggccctct ttgtgaccga gatccacaac gagatccggc gcgtgaataa cgaggggcgc
    10501 gtgctctcgg agatcatcgc cgacggtgcc gcgtcgccgc tccgcttcga cgatccccgt
    10561 gtggcggaca gcctgcttgc ctccctgcgt caccatgagc gggtcgcgac ggccctgctg
    10621 gtacgaccgg acggccgtgt cttcgccgcc taccctgccg gtgtcggcgc cgcgcaggcg
    10681 cgggtggagg cgctcaaggc cgccgccgag ccgggccgct tggggtgggg gctgacccat
    10741 ctggtggtcg cccagggcat cgagagcgat ggggagaacc tgggccggct gatcatcgaa
    10801 ttcgacctgc acccggtcct cggccaattc ctggcctggt tcggttatgc cctgttcggt
    10861 ctgggcggtg ccttgctggc ggcctccctg ctgacccgtc gcatgcaggc gatgattgtc
    10921 gagccgatcc agcgcctggc ggaggtcatg cgcgaggtgg gccgctacaa gcgctatgac
    10981 ctgcgggcgc ccgccggcta cgcggatgag gtgggtgagc tgatcgccgg cttcaacggc
    11041 atgctggccg agatcgccac ccgtgaccag gaactgaccc tgcatcgggc gcagctggaa
    11101 tccgaggtgg cggtgcgtac ccgcgagctg gtggctgcca aggaacaggc cgaagcggcc
    11161 aaccgggcca agtcccagtt cctggccaac atgagccacg agatccgcac gcccatgaac
    11221 ggcgtgatgg gcatgatcgg cctgttgcgc cagacctccc tggcggcgcg ccagggccat
    11281 ttcgtggaca tgctggacga ctcggcgcgg gccctgatgg aggtcatcaa cgatgtgctg
    11341 gacgtctcca agatcgaagc cggccgcctg gagctggagt ctctggcgtt ttccctgcgc
    11401 gacaccgtgg accaggtggc ctccctcttc gcgacctctg cccagcagcg cggcattcgc
    11461 ctgcgcctga aggtcgaaca ggatgtgccg gacatggtgc gcggcgacgc cctgcgggtc
    11521 cgtcaggtga tcagcaacct ggtttccaac gcccagaaat tcaccgagca cggcagcatc
    11581 gccatccagc tgcgccggct gccctgcgcc caccccggtc gggtgcgcat ccgcgccacc
    11641 gtgcgcgaca ccggcatcgg cgtgccgcgg ggcatggaat cgcgcctgtt ccagtccttt
    11701 tcccaggccg acaattccat ggcccgccgc ttcggcggca ccgggctggg gctgtccatc
    11761 gccaagcagc tggtgaccct gatggatggt gacatcggct acgattccgc gccgggcgag
    11821 ggcgccacat tctgggtgga gatcgagctg ggcgccgtga cccagggcga cgcccacgcg
    11881 gtgctcgacg gtcgccgggc cctgctggcg atgggcgacc cggcctcgcg ggaggcgctt
    11941 gccgaacaga tcgtcttcat gggaggtgcc gtcgagatcg cgcccgacca ggcggccgtg
    12001 acggcccggc tggcctccgg tgccgtgttc cagtggatcg tcaccgacgc aggcttcgac
    12061 ggtggcaagg gctttgcgtt gctcgagatg ttgcgggagg cgccgtctgc gccgccgcgt
    12121 ctggctgtgg taggaacagg ccgggaggag gccgagcagg cccgcatcgc cggcgccgcc
    12181 gaggtgctgg ccggtccgct caccggggtg gcgatccggc gtgccctcct cgggcaggag
    12241 cgggacccgg acgacacgtc cgtggtgccg ctgaccgccc gcgtgctggt ggccgaagat
    12301 catccggtga accgggagat cgccgtggcg gtgctggaat ccctcggttg ccgggtggcc
    12361 gtcgccgaga acggccgcga ggccttcgag gcctgccgcc gcgagcgctt cgacctggtg
    12421 ctgatggaca tccagatgcc cgagatggac ggtcgccagg cgaccgtggc gatccgcgcg
    12481 gatgaggatt tccgctgtct gccgcgggtg ccggtggtgg cgctcaccgc caacgccctg
    12541 cgggaagacc gggacgcctg cctggcggcg ggcatggatg actacctggt caaacccgtg
    12601 tcgcgggaac aactgggcga aacgctggcc aaatggctca ccaccttcgt gccggtcgag
    12661 cccgcgccgt ccctgctggc atcgccgcga ccggctccgt ccggccatgt ggaggcgccg
    12721 gcggcgctgg acatggatgt gttgctcggg ctgccgggcg tcaaccgcaa tccgtcgtcg
    12781 cccatgctgg tccgcctggt ggacctcttc gccaccgaaa ccgaacggca cgtggccagc
    12841 gtgcgcagta gtttcgaggc aggcgactgg agcgcgatgc gggccgtggc ccacaagatg
    12901 aagtccgggt gtctggccct cggggccgtc cgtctggcag gatgtgcccg tgccctggat
    12961 gatcacatcc gcgaaggcgg cgtgccggcg gccggcgagg tcgaggccct ggcggaagcc
    13021 tggcaggcct gccgggcggt gatggtggcc cgcgggctgc ttgcagcggg tgaggcggag
    13081 cgcgccgtcc cgccgtcccc ggatacaccc ccgaattctc cggcgccgac cgcgccccaa
    13141 gaccatgact gaagcgtccc gtgcccgcat cctgatcgtc gacgacgacc cgctgacacg
    13201 cctgatggcg ggcgaagccc tgcgcgaggg ggggtacgcc gtgcaggagg ccgaggacgg
    13261 gcagcaggcg cttgagcttt tctccgcgcg gcggcccgat ctggtgttgc tcgatgtggt
    13321 gatgccggga ctcggcggtt tcgaggtgtg tgcccgtctg cgcgcactgg atgccggcct
    13381 gctggtcccc atcatcatgc tgacggggct cgatgacagc gagtccgtgc agcgcgcctt
    13441 cgatgcgggc gccaccgact tcatctccaa acccatcaac tggaccctgc tgcgcttccg
    13501 ggtgcgttac gtgctccgct ccgccgacgt gatgaaggag ctggtgcgca acaaggaaag
    13561 cctctccaac gcccagcgcg tggcccgcct gggcagttgg gaatggtggc ccgagcaggg
    13621 cagcatccag cgctccgaac agtatttccg gctcttcggg cagaccgcgg cgagcttcgg
    13681 cgatgacgcg caggccctgg tcgatcacgt ctatctgccc gaccgggcca ccgtggcggc
    13741 cgccctcgac gaggcggcac ggggacacgg ctatcagctc acctaccggg tggtgtggcc
    13801 ggacggcagc gtgcgcactt tgcacgagac ggccgaaccg gcgccttcct ccgatccggc
    13861 gcgggggcgc tgggtgatgg agggcagcgt gcaggacatc acccagcagg tggaggccca
    13921 gcggcgcatc cgccagctgg cctatttcga ttccctgacc ggcctgccga accgggagtt
    13981 tttccgtgaa agcctgctgg cggcggtgag ccgctgccgc cgcaacgaga ccggctgcgc
    14041 agtgatggtg ctggatgtgg accggtttgc gcgcatcaat gacagcctcg ggccggaccg
    14101 gggcgaccag gtgcttcagg tcatcgccca ccgtctgcgc gaatgcatgg gcgaccgcgc
    14161 gccggtcgag cccgccggcg gtggcggctt gccagacctc aaggtggcgc gcctggcgtc
    14221 ggacgagttc gcgattctgg tgcaggaggc cggatcaccc agcatgctgc tgggggtggc
    14281 tgagggcttg ctggccacgg tgcgggcgcc gatccatgtg ccgggacagg agatcgccct
    14341 gtcggcgcgg atcggtgtgg cgatgatgcc cgaccacgcg agcgacgccg acgccttgct
    14401 gaaggcggcc gagaccgccc tcaaccgggc caagacccag gccggcgagc aggtggcgct
    14461 gttctccgag gagatgaagg tggcggcctt cctgcgcttc agcctggaaa gcgacctgcg
    14521 ccgcgccatc gagaacaacg aactgaccgt cgtgtatcag cccatcgtgg acgtgcgccg
    14581 gcgggccatc gtcaaggccg aggccctgat ccgctggaag cattccctgc gcggcatgat
    14641 cccgccgccg gagttcatct ccctggccga ggaatccggc ctgatcctgc ccctgagccg
    14701 gcgcctgatg gacaccgtgt tcgccgacgt ggcccgcctg gagccggtgg ccccggaggg
    14761 attccgggcc agcgtaaacc tctccggggt gaacttcatg gacgtccagc tcatcccgat
    14821 ggtgaatggc ctcctggccg atcgcggcgt acccgcgacc cggctggagt tcgagctgac
    14881 cgagacggtg ctgatgcgcg acctggacta tgcgaccacc atccttgcgc gcctgcggga
    14941 gatgggcgtg cgggtggcgg tggacgattt cggcaccggc tattcctcgc tggcctacct
    15001 gcacagcctg gcggtggaca ccctcaagat cgaccgctcc ttcgtgaatg aactggacag
    15061 cggtcagggc ggggtgatcg tcgaagccgt catcggtctg gcccagagcc tgggactcga
    15121 cgtggtggcc gaaggcgtgg agacggcggc ccagctcggt gccctgcgcc agcgcggctg
    15181 ccacctcatc cagggctacc tgttcgcgcg gccgatgccg gtggacgacc tgcagcgcct
    15241 gctgcgcgac ggggccgatt tttcgtccat cctcgcctga gcgcgccgct cagatccgcc
    15301 agcggatgcg ttccttcatc atcgcggcaa tggcctgggc gcgctccggc gagtccgcca
    15361 cgatggcgta atacccgtcg ccgcccggct gcgccagcgc ggtcattcct ttccattcgg
    15421 cctccagcgg cttaccctgg gcgctgccct cgccggcctt gcgcgggatc aggagcaggg
    15481 tcgcctgccc atgctcggtg cggatcacga tgtgccagcc ggtcccgccg gggaccgggc
    15541 acagcttcat gtacaacacc tcgcccaccg gtgcttccag ttccgccccg aagcgggcga
    15601 gggccaggcg gaactggctc ggatccgcca ggcggtggtc ggtgaaggac tcgggttcgt
    15661 gcagcgtgtg atcgatggcg aagcgcgcgt agtcgtcccg cgggtagtcg tgccacaggt
    15721 tgaagctcag cccgacgctc agcaccaccg tcgccgccag agccagaaag cgccaggggc
    15781 gcggccggcc gtggtggacg cgcaccagga cgcggtctgc cagcccgtcg gggagcggga
    15841 tgtcgaggac cgtggcgatg cgttgctcct gcgtatcgac gcggcgggcg aaggcctggc
    15901 acagggcgca ttcgcgctgg tgggcgaggg cggcggcgga caggcgatgg ggatcggcca
    15961 gcttgaggcg ccggaattcg aggcagttca tgggcgaggc gaagacgtcg cgggcggggt
    16021 tctgggcgtt catgaggcac tcccggtgtg ccggggggcg gcggcaccgt ccagcagctg
    16081 gcgcaggccc tggcgggcgc gggtgaggcg gaccatcacg gcgccttcgg tggtgtccag
    16141 ctgggcggcg atctcgccgc aggaaaagcc gcccagagtc tggagcagga agggttcccg
    16201 cagggacagg ggcaggcggc cgatcaggtc ttccagttcg tgcagggcga ccgggtcgtg
    16261 gtcggcggcg atggccagct cttccggctg ttcgtccacg tagtcgaact gcttgcgctc
    16321 gtacaggcgc gcgtgctcgt tgcgcaggat ggtgagcagc cagggcttgg gggcggactt
    16381 gtcgcgcagg ctgtcccagt tgctccaggc acgggcgaag ctctcctgca ccaggtcttc
    16441 ggcgacgaag cggtcgcggc acagccagta ggcaaaacgg tacaggtcgg cgctgtaggc
    16501 gcgcaccacc tgttcgaatt cccgtttgcg ggtgaaggag aagatcatgg tcgtcggagc
    16561 gtccggcagg ctgtcttgcc ggagatgacc ggaccggacc ggcaaagctt acatcgacgc
    16621 ggtcgtcttg cgttcggcgc ggatcgggat cgccaggcgg cggccctcgg aggcctccag
    16681 ttcgcgcagc tggcggatca ggccgtcgtc gaggacgaag tcggcagcga gaagcatgac
    16741 gccttccggc gtcaccagat cgcgggcgag caccatgccg ggcttgagcg ccgccgtgct
    16801 gacgatgttg cccaccgccg gggcactctc ggtcgagccg aggaccgccg cgagggcgtc
    16861 caccgattgc gggcagtaac gcttgccgcg attctgctgg atgaaggcga gggcctcctc
    16921 ctgacgcagc ttgcggggcg tgagggtgcc gatctgcagg ccgtcgaagt cgttggccac
    16981 tgccagcagg cgggcgccga tggggatggc caggccggag aggccgtccg gatagccctg
    17041 gccgtcccag cgttcgtggt gggcgcggat cagggtgccc gccttgcgca ggttttccag
    17101 ggccatcagg gcggactggc cggccaccgg gtggcggcgc agctggccca gctcgtcgcc
    17161 ggtcatgtgg gacacgggct tggacagcag ctcgtcacgc aggccgatct tgcccacgtc
    17221 gtggagcagg ccggcgagca tcacgtcctg ggcgtcggtg gcgttgaagc ccaggtggat
    17281 cgccatcttg cgggccaggt cggcgacccg ccgggagtgg ccggacatgg cgccctcgcg
    17341 cagctcgatg aggttggaga acacccggat cgaggtgagg aagttctgct tgagcttgtc
    17401 atgggcgatg cgcagctcgg cggtgcgggc ctccaccttc tgttccaggc tggcgttgag
    17461 ggacttgagc tcgtcgttct ggctgcgggt gagggcttca aggcgggcct tctccttttc
    17521 gagctgcttg cgttccaggg cttcgcggac ggtgaggagc acgtcctggt cgttccacgg
    17581 cttggaaatg tagcggctga tctggcccgc gttgatggcc tcgatggtgg attccatgtc
    17641 ggcgtagccg gtgaggagca ggcgcaccgt gtccgggtgg cgcttgcgcg cttcgcccag
    17701 gaagcgggcg ccgtccatga ccggcatgcg catgtcggag atgatcaggt ccaccggctc
    17761 gcgggcgagg atttcgagcc cttcctcgcc gccgccggcg gtgagcacgg tgtagccgtg
    17821 ggggcggaag aggcggcgca gggccgacag gatgttggct tcgtcatcca cgcacagcag
    17881 ggtgaaggcg ggggcggcgg cttcgctcat ggggtggctc cgtcggtgag ggcgcggtgc
    17941 acgggcagga cgatgcggaa ggtggtgccg gcgcccggcg tgctggtgac gccgatgtgg
    18001 ccgtggtgct tctggatgat gccgtaggac agggacaggc ccaggccggt gcccttgccc
    18061 accggcttgg tggtgaagaa ggggtcgaag atgcgcgcca tcacatcggc gggcatgccg
    18121 ctgccgttgt cggctacttc gatccacacg gcctcgcggg ttggttcggt accggtgcgc
    18181 acggtgatga ggccgcggtg gccctcgggc atggcctggg cggcgttcac gaagaggttc
    18241 atgaagacct ggttgagctg ggacggcagg cattccacga ggggcatctc gccgtagtca
    18301 cgcacgactt cggcgcggta cttgatctcg ttgctggcga tgttgagggt gctgtcgagg
    18361 ccacggcgca gatccaccga ctgccagtcc tgggtggcct cggtgcggga gaagtcgcgc
    18421 aggtcctgga cgatcttgcg cacccgttcg gtgccttctt cggattcggc gaggagctgg
    18481 gtgacgtcct cccgcagaaa gtcgaagtcg gcggccttgc gcgccgcagc gagggcggcg
    18541 cggtcaggtt ccggcagggc cgcctcgcgg gcctggtagg cgtccaccac gcccagcaac
    18601 tgggcgatgt agcttttgag gctgcggatg ttggaattga cgtagccgat ggggttgttg
    18661 atctcgtggg cgacgccggc ggcgagctgg ccgatggagg ccatcttttc ggactgcacg
    18721 agctgctgct gggtgtcgga gaggcggctg ttgaggtcct tcagctcgga gtagcgggcg
    18781 cgcaactcgg cctcggcggt ctcgcggcgg gcaatgtccc gctccagggt ggccttggcg
    18841 tcctgcagtt cgacggtgcg ctgggccacc ttggcttcca ggctggtgtg catctggcgc
    18901 agttcgtcct cggccttgcg ccgctcggtg atgtcctgca gcgtttcgat ggcaccgatg
    18961 gtggccccgg cggcattgcg cagcggcgcg gcggtgaagt acagccagcg gccgttctcg
    19021 ccgaaatggg ggaagaagtc ttcggcctcg taggcgctgg ccaccactgg cgagcggcgc
    19081 agcttcttct ggccgtagtg ccgggtgatg gtctcgatct cgccggagac gatgaggtcg
    19141 gccatgatgg gccgctcgtg gtcatagaag gccgtccagt gcttacgggt gcccacgatg
    19201 tcggcggcgc ggatgccggt gatctgctcg caggcctggt tccagtgggt gacgatgtgg
    19261 cgggcgttga tgaccagggt cggtaccggg tcgccgtcga tgatctgggc caggagctga
    19321 cgcgactggg cctgggccgc ttcggcgcgc ttgcggtcgg tgatgtccag gtagctgcac
    19381 agcacggtgc gggcgccgtc gatggcctcg caggagaggg tcagttcgac ccagcgctcc
    19441 tcgccgctct tgatgacgat gagggtctcg tagcgggccg gcggatgctc gccctgcagg
    19501 cgcatgcgtc cccattcctg caccttgccc tgggccgcct tgggaaagaa ggcccagaag
    19561 tccatctgcg cgagctcgtc cgccgtgtaa ccggtgagcc gcgacatggc ccggttggcg
    19621 aagaggatgc ggtcgctgcg gtgcaggacc agacccgagg gcagggagtc ggcgagcggg
    19681 aagaggctga ccggcaaggc gtagggacgt ggattcatcg gctcacccca gcaggaggca
    19741 gtaactgtcg aaacgcgccc gggtgccggc gagcacgcca tgaaggtcgt cccgccccag
    19801 gcccagggcg tcgacggtgg cggcgtgcag gcgggggatc tggctgtcgg tctcgccgtc
    19861 caggtcgagg gcctgggcga tggcgtcggc gtagtggagg acgccggcca gtccgccggg
    19921 ctggctgcgc tccggtgcgt ggtggcaggc cagggcatcg gcgatctcgt ccggaaagtt
    19981 ccagcggcgg gcgagggcgg cgccggcctc ggcgtggtcg aagccgaaga tctccagctc
    20041 cacgtcgatg agctgtgcgt cccgttcgcc ggcccggcgc agcacctcgg cgtagtcggc
    20101 cgggtagagg gtgaccaggg cgaggcggcc gatgtcgtgg atcaggccgg ccaggaagag
    20161 gtgcccgggt gagcgcctga gggccttacc catggcttcg gcggcggcag ccgtgccgat
    20221 cacgtggtgc cagaagcgtc gttcatcgaa acccgggcag tgtccggcgg gcagggcggt
    20281 gaccacggcc gccgccagga ccagggagcg tacggcggag aggcccagca ccatgatcgc
    20341 gtcgtggatg ctggccaccc gcccggccat gccatagaac ggagagttgg ccacccgcag
    20401 cacccgcgcg gcgatggcct ggtcctgggc gatgcggcgg gccagcgcgt ccacgtccag
    20461 gtcggcgccg ttgagggact ggaggacttc catcgcgacg gccgacatca ccggcaggcg
    20521 ctggatgcgg gcgagggtgt cggtcagggt cggggcggcg gtcatcggat tcatttcagc
    20581 tgctcctgac gaaaggcgag gaccgcgtgc aacagcgcct gggcgccggg atcgtcggcc
    20641 tgatggcgga acagatgcat cacgcgggca cggatcgctt cgcggcggcg ttccagctcg
    20701 gcggcccgct cagcctcctc ggctgcccca tcgggggggg tgtcgacatg ttcggtggct
    20761 tcgctcatgc gccactcctg tccgtgaagc tcaagagagt gccgcggcag gtgcccacgg
    20821 catggagggg atgagccacc acgtgaaagt ggcgggcgcc gtcccggtag tccgttgccc
    20881 cttcgccgcg cagggcgaga tcgcgcaggt cgggcggcag ggcttcgtcc acgcccagcc
    20941 cgatgagggg cgcgtgggcg ggaaagagtt tgtcagctgc cgcgttggca aaggcgatca
    21001 tgccgcccgg atcggtgccg agcaccggcg cgggcagcgc cgcgagggcc gcctgggtga
    21061 ggtgcagggc cgactcgtcc acgtccagct gacgctcccg ttcggcgagc agggcggcca
    21121 ggcgttcgtt gatgcgcgcc agctccgcgt tggcccggtg cagctccgcg tccaggcgca
    21181 ggttctcgtc caccagggcc ttgcggtgga aggcctcgtc gatgttggcg cgcagcatcg
    21241 cgtcgtccca gggcttggtc aggaacttgt agatgctgcc ctcgttgatc gcgtcggtga
    21301 cggactgcag ttcggtgtag ccggacaaca ccatgcgcac ggtgtccggc accatgtcct
    21361 tggccttgcg caggaactcg acgccggtca tgccgggcat gcgctggtcg gagacgatca
    21421 cgtccaccgg gtgggtggcg aggaggtcga gcgcctgctg gccgctgctg gcggtgagca
    21481 gggtgtagcc gtcccgccgc agcagtcggc gcagcgcgtt aaggatgttc tcctcgtcgt
    21541 ccaccagcag cagcgtgcgg tccgggcggg gaccacggat cagttccggg ttgaggcgtt
    21601 caccggcggc cagcagggct ccgaaggcct ccgccgtcat cgggccgccc aacagcggcc
    21661 cctgcacgaa gtcgcagtcg ctggccacca gcacgccggc ggtgtggcgg tcggcgatgc
    21721 ccttggcgct gacccgctgc cccatcttgt gggcgcgggt caccagggcg cggatgatgg
    21781 cgacgccttc aggatcggcg gtcacgtcct gcaccagcgc ggcggggact tccaggcaat
    21841 ccaccggcag acggcgcagc caggcgaggc gggcgttggc ggggctggcg gcactgatgg
    21901 caaagcgcac gccgcagcgt ttgaggtggt gcagggcggc ggcctgctcg tcgggaatct
    21961 cgccggtgcg ggtgatgatg ttgagctcga ggctgtgggc gtcgaggcgg gcgtcgagga
    22021 tcagaccgtt gagggcctgg cggaaggtgg gggtggcgag gacctcgagg gtcgcgccga
    22081 gggtcaggat caggtcgagt ccgtggtggg cgttccagtc cgccacctgc cggcaggcgc
    22141 tgtgcaggcg ggcgaggcag gcgtcgtcga gggcggtgag cacgacattg tcgctgcgct
    22201 gcgggcgggc cacgtacagc ttggcgccca ccagcgcgcc gtcacggcag tcgtaacggg
    22261 gctgatagtg cagggcgggc gggatggcaa ggctgtccat ggcgtctccg gttgcagcgt
    22321 ggcacactcg ggtgtgctcc gatcgttctg tcgccagtaa cgaccggtca tattcgaact
    22381 ttagggtcgt attttacgtt tttagtgatt ttattgtttg agtttgtttt tatcagattg
    22441 ttccgacggg cggaagtctc cgtgtgtact ctgcagccta aagtgagggc gtgactgtcc
    22501 gccggtcccg aggaggtctc catgtcgccc ggatcccccg ccaccatgcg cgccatggtg
    22561 ctgcaccagc ctcgtacgcc cctgcagctc gaacgcctgc ccgtccccgc gccaccgccc
    22621 ggtacggtgc gcctgaaggt ggaggcctgc ggcgtgtgcc ggaccgatct gcacctgatg
    22681 gagggcgagc tgccggaccc ggtgtggccc atgatccccg gccacgagat tgtcggccgg
    22741 gtggatgtgg tcggcgccgg cgtgagtggc ttcgcgcctg gtcagcgggt cggcgtgccc
    22801 tggctggggt ggacctgcgg cacctgtggc ttctgccgga gcggccgcga gaacctgtgc
    22861 gctcaggcgc gcttcacggg ttaccagatt cccggcggtt acgccgaata caccgtggcc
    22921 gacgcccgct actgctttcc gctgcccgat gggggcgagg cggccgcgct ggcgccgctg
    22981 ctgtgcgccg ggctgatcgg ctaccgtgcc ctgcgcatgg ccgacgacgc gcagaaggtg
    23041 gggctgtacg gcttcggtgc ggcggcccat ctcgtcgccc aggtgctgcg tcaccagcag
    23101 cgacccttct acgcgttcac ccgtcccggc gacatcgaaa gccaggcctt cgcccgtcag
    23161 ctcggcgcca cctgggtggg cgatgccgcg acgccgccgt ccgagcgcct cgacgccgcg
    23221 ctgctctttg cgccggccgg cgccctcgtg ccggcggcgc tgcggcatgt ggacaagggc
    23281 ggctgcgtca tctgtgcagg catccacatg agcgacatcc cgtcctttcc ttacgccgac
    23341 ctgtggggcg agcgcacgat ccgctcggtg gccaacctga cccgccggga cgggctggaa
    23401 ttcctcgcac tggcgcccca ggtgccggtc cgcgcagaga tcaccccctt cccgctggcg
    23461 cgggccaacg aggccctcga tgccctgcgt cgcggcgccc tgcagggggc ggccgtattg
    23521 acgatggact gacccctccg cggcggggcc caggccgatc ccgcccggtc cgacctccgg
    23581 cagggtagaa tccggcccca tgcccgacca cagtcattcc ttcccgaccc tcgccctcgt
    23641 cgccatcggt gtgggcgccg cgctgggcgc gtggctgcgc tggtggctgg ggctgaggct
    23701 caatccggtg ctggcccaga tgcccttggg tacgctggcg gccaatctcc tcgggggcta
    23761 tctggtgggg gtggcggtgg ccttcttcgc cctgcgtccg gacatcccgc cggaagcccg
    23821 cctgctcgtg gtgaccggtt ttctgggtgg gctcaccact ttctcgtcct tctccgccga
    23881 ggtcgtgcac ctgatctcca cagcccgcta tgcctgggcg gcgggggccg cggcggtgca
    23941 cctctttggc tccctgacca tgaccgccct gggcatgtgg accgtccacg ccgtccgggg
    24001 ctgatccgct ccgtttcacc ctcgaacacc catggctgca ccccacgacc ggcccgacgc
    24061 cgtccggcaa gatccgtact ccctgctcgc acactgcctg acggccgacc ggccggccct
    24121 gcgccgcgac ctgcgccgcc tgccgcgacg cccccgcacg ctggaggatc tgcccgaggc
    24181 cctgcgcctg cgcatcgccg attccgccgc ccgccgcatc gcccgcgagc aggcccgtcc
    24241 gcgcccggac tttcccgacg agctgcccgt caaccagcgc cgcgacgaga tcgccgccgc
    24301 catccgcgac caccaggtgg tcatcgtctg cggcgagacc ggctccggca agaccaccca
    24361 actgcccaag atctgtcttg agctgggccg cggcgtcgcc gggctgatcg gccacaccca
    24421 gccgcgccgc atcgccgccc gcgccaccgc cagccgcatc gcccaggaac tcaggaccga
    24481 gctgggcagc gccgtcggct acaagatccg cttcaccgac cgcatcggcc cccagagcta
    24541 catcaagctc atgaccgacg gcatcctgct ggccgaaacc cagggcgacc cgctgctctc
    24601 cgcctacgac acgctgatca tcgacgaggc ccacgagcgc agcctcaaca tcgacttcct
    24661 cctcggctac ctgcgccagc tcctcgccaa gcggcccgac ctcaagctca tcgtcacctc
    24721 cgccaccctc gacgcggagc gcttcgcccg ccacttcggc acgccggagc ggcctgcgcc
    24781 ggtcatcgag gtgtccggcc gcctctaccc catcgagctg cgctatcgcc cggtggagcg
    24841 ggacgccccc aagccgctgc gccccggcga ggcgccacgc cccgagcccc gcggccccga
    24901 ccgggacctc tacgacgccc tggtcgatgc ggtggacgaa gcccagcgca gcggccccgg
    24961 cgacgtgctg gtgttcctgc ctggcgagcg ggagatccgc gaggcggccg aagccctgcg
    25021 caaggcccac cacgccgccg gcaccgagat cctgcccctc ttcgcgcgcc agtccgccgc
    25081 cgaccaggcc cgtgtcttcg cccccagcaa ggggcgtcgg gtggtgctcg ccaccaacgt
    25141 ggccgaaacg tccctcaccg tgccgggcat ccgctacgtg gtggataccg gcctcgcgcg
    25201 ggtcaagcgc tatgcccacc gcaacaaggt ggaacagctc cagatcgaga agatcgccca
    25261 gtccgccgcc cgtcagcggg ccggccgctg cggccgggtg agcgacggca tctgcttccg
    25321 cctctacgac gaggacgact tcaacaagcg ccccgcccac accgatccgg agattctgcg
    25381 ctcctccctg gccggcgtga tcctgcgcat gaaggccctc aagctgggcg aggtggcgga
    25441 cttccccttc atcgacgcgc ccctgccgcg catggtggcc gacggttacc agctcctcgc
    25501 cgagctgggc gcggtggagg agggcggtgc cgacgaggcc ggcagcgccc gcatggcgct
    25561 cacccccatc ggccgcgaac tggcccgcct gcccctggat cccaagatcg gccgcatgat
    25621 cctggccgcc cgcgaccggg gctgccttgc cgaggtgctg gtcatcgccg ccgccctgtc
    25681 cacccaggat ccgcgggagc gcccgcccga acgccaggcc gccgccgacc aggcccacgc
    25741 ccgcttccgc ggccccgagc acgagcagaa gtccgaattc ctgtggttct ggaacctgtg
    25801 gaaagcctgg gacgaagttc tgcgccacga aagcgccagc aagcagaaag cctggtgcaa
    25861 gcagcacttc ctgtcctggc tgcgcctgcg cgaatggcgc gacgtgcatg gccagctcca
    25921 cgccctgtgc gccgagcacg gctggaagga gaacaccgaa cccgccgcct acgaggccct
    25981 ccacaaggcc ctgctcgccg gcctgctcgg ccacgtgggc tgcaagatcg aggacgccac
    26041 cggccccgcc gccggcagct acctgggcgc ccgcggcatc aagttctggc cccatcccgg
    26101 ctccagcctg gccaagaagg ccggcaagtg gatcatggtg gccgagctgg tggatacctc
    26161 gcgcctcttc ggccgctgcc tcgcccgcat ggaacccgag tggctggagg aggtcggcgc
    26221 ccacctcctc aagcgccaga tctccgaacc ccactggtcc aagagccacg gcgccgtgcg
    26281 cgcctgggag cgcgccaccc tctacggcct caccgtctac gcccgccgcg gcgtgagcta
    26341 ccgggacatc gacccggcct tgtgccgtga gcttttcatc cgcgaaggcc tggtgcaggg
    26401 cgaaatcgcc gaaggccccg cgcggggcat ggccttcctg gcccacaacc gccgcctcgt
    26461 ggccgagatc gaacgcctcg aacacaagtc ccggcgtccc gacgtactgg tggacgaagc
    26521 gctcatccac gccttctatg acgccaagct cccagccgac gtggtggatc tggccagctt
    26581 cgaagcctgg cgcaaggacg ccgagcgcaa ggcgcccaag gtcctgcagc tcacccgcga
    26641 ccagctcatg cgccacgacg cggagggcat caccaccgac cgcttccccg cccacctgga
    26701 cgtgctgggc cagaagctca agctcaccta tctgcacgcc cccggcgagg ccgacgacgg
    26761 cgtcaccctc accgtgcccc tggccatgct caaccaggtg cccgcccacc gctgcgaatg
    26821 gctggtcccc ggcctgctcg aagaaaaggt caccgccctg ctgcgcaccg tgccgcagaa
    26881 gcaccgccac cgcctgcagc ccatggccga cagcgcggcg gccttcatgg agcgcttcga
    26941 agccggcgaa ttcgatctgg atgagccctt cctcaaggcc ctgcaaggtt tcgtggagga
    27001 gcgggtgtcc ctcaagctgc ccatggaaag cttccggccc gagaacctca acccccactg
    27061 cttcatgcat ttccgggtgc aggacgaaca cggccgcgtc ctcggccagt cccgcaacct
    27121 ggccgagctg cgcagccgct tccgcgatca ggtggccgcc cgcttccagt ccgcccgcat
    27181 cgtccctgcc gccgacgtac cggctacggt cgccccggtc ggcaaggcgc cggccgcggt
    27241 cgacgccacg tcgcccgtcg cgggcggtgc tctggcgggc ctcaccgggt ggaccttcgg
    27301 cccgctgccc gagttattgg aggtgaaggt cgccggccgc gagatcgtcg gctttccggc
    27361 cctccacgac gacggcgcca gcgtctccct gcgtccctac gacacgccgg aggaggccgc
    27421 caaggtccat cgcggcggac tggcacgcct gttcgccctg gccctcgccg cccaggtcaa
    27481 ggccatcgaa aagctgccgg gcctgcgcga gctggccttg cagttcctgc cctacggcac
    27541 cgaggccgaa ctcaaggccc agctcgtcgc cgccaccctc gaacgctgct gcctgatggc
    27601 accgctccct gccgacgccg atgcctttgc ccgctgctgc caggacgcca agccgcgcat
    27661 caccctggtg gcccaggagc tcatgcgcct cgccggccag ctgatcgccg agcacgccac
    27721 cctgggcaaa cgcctcgccg gcctcaagac ctttcccgag gtggtcgccg acatcaccgc
    27781 ccaggccgcc cgcctgatgc ccaaggactt catcgtcgcg ctgccctacg agcgcctcgc
    27841 ccaggtgccg cgctacctca agggggccag cgtgcgcatc gacaagctgc gcaacaacgc
    27901 ggcccgcgac gcccagctcc aggccgactg gaagcgcctc gcccagccct tcgagcggga
    27961 atggcttgcc aaggcccgcg ccggcgtcac cgatccgcag ctcgaagagt tccgctggct
    28021 gctggaagag ctccgcgtcg gcctcttcgc ccaggagctc aagacgccca tgcccgtctc
    28081 ggtcaagcgc ctgcaaaaga tctgggacag ccggccgcgc tgaccgcggg cgtcacgtca
    28141 tcaaatttga cgatgtccgc ccctctgtct ataaaagaat tgacccgcaa tgggcacagc
    28201 ctccacatcg gtggagaaca cggaaggccg ccaatgagcg gccttctgtc tttgtggggg
    28261 cgatgcaggg ggagatggcg gcgggccgat ctggcgcccg cgcgggcgcg gatcgccctc
    28321 cggtcccacc gcggggcagc gcgcctgctt caggacgcgt actcgccttc cagcacccgc
    28381 agcacctccc catacaaggc ctccggatgg attggtttgg caatgaaggc gttcatgccg
    28441 gcctggcggc agcgggcttc gtcctcggca aaggcgttgg cggtgagggc gaggatgggg
    28501 gtgcgggcgt ggcgggggag ctggcggatc tggcgggtgg cgtccaggcc gtcgaggcgg
    28561 ggcatctgca tgtccatgag gatgacggcg tagtcgttgt cgcgggcctt ggcgacggcg
    28621 gcttcgccat tttcggcgat gtcggcgacg aggcgcgctt cttccaggag gagttgggtg
    28681 atctcctggt taatgggttc gtcctcggcg aggagcacgc gggcgccggc gtggcgggta
    28741 cttagggcga gcagggtggc ggccgggtcg ggccgggcgg cctccgccgc ggtcggaccc
    28801 ttgcgcagcc gcgccgtgaa ccagaaacgg ctgccacggc cgggctggct gtcggcaccg
    28861 gcttcgccgc ccatgatctg ggcgagcttg cgggtgatgg cgagacccag accggtgcca
    28921 ccgtagcgcc gggtggtgct gttgtcggcc tgctcgaagg cgttgaagag gcgcggcagc
    28981 gcgtcggcgg tgatgccgat gcccgtgtcc tccacgtcga agcggaccag gacgtcggcc
    29041 ggtccgtcct ccacgatgcc ggcgcgcagg acgatctcgc cccgctcggt gaatttgacc
    29101 gcgttggcgg tgtagttgag gagggcctgc tgcagacgcg tggcgtcgcc cagcaggttc
    29161 gcgggcagcg gaccgacttc gctgcgcagg acaacgccct tgccggcaga gcgccactgc
    29221 agcagggcga ccacgtcggc caccacctgg gcgaggcgga ccggctgttc ttcgagggcg
    29281 aacttgccgg cttcgatctt ggagagctca agcacggcgt tgaggatctc gagcaggtgc
    29341 tgaccggcgg tctcgagctt ggcgaggcgc tcggcctgct gggggctgag gggctccttg
    29401 cggatcaggt gggccatgcc cgtaatggcg ttgaggggcg tgcggatctc gtggctcatg
    29461 ttggccagga aggcgctctt ggcgaggctc gcggcttcgg cggcgttctt ggcgtcctgg
    29521 agttcgcggg tacgttcgtc gacccgctgt tccaggtggg cgcggtagtc ggcgagctcc
    29581 tcgtccagca ggcggcgggc ggtgatgtcc tggatggtgc cgaacatgtg cgtcgggtgg
    29641 ccggtggcgt cgagctcgag ttgcccacag ccgagcaccc agcgcaggct gccgtcgcag
    29701 cgccgcagga tgcggtactc ccgattgaag ggctggcgct gggtgaggac gtggtccagc
    29761 aggtagcggt gcatcccgtc ccggtcgtcc gggtggatga gatccagcca gtcctggagt
    29821 tggcgatcgg gcgcgggggc gaggccgaag atgtcgtcga ggacttcgga cgcggtccag
    29881 cggccgctgc ccacgtcgaa tttgtaggac ccgatgtggg cggcgcgctg ggattcgcgg
    29941 aagaagtatt cgctttcccg cagggcggct tcggcgagca ccttgtcggt gatgtcccgg
    30001 gcaattccga acaggccgat gatccgccca tcggcgtcga gtacggggta tttgcggttg
    30061 tccacccagc cgcgggtgac accgtcccga tggaggtagg gctgcacttc atgggccacc
    30121 tgggcgccgg cgaagacctg cttttccagg cggtagtaga cgtcggcgta ggcttcgggg
    30181 aagacgtcgt agtcggtctg accgagaaag tcggtccagt gacccaccgg ctcgcacagg
    30241 ctcacgaggg tctggctggc ggcggcaaag acgtggtggc gatccttgaa gtagatgtag
    30301 tcgtcggtgt tctcgagcat ggcccgcacg ctgagggtgg cgacgtcgtc gagggtccgg
    30361 ggcaggtcgc ggctgtcgct caggtgcagc tcgaaatgct ggccgtcggc gctggcgtga
    30421 tgggtgcagg tggcgcaggt gacgacgatg cgtccatccg cccggcgcag acgcaggtgg
    30481 agggggccgt cggcggcggg ggcgtggggg tcgaagaggc gggcggccac gtcctggtcg
    30541 tcgggatgga tgcgctcggc caggcagacg cggccgtcgg tgagggcggc ggccgactcg
    30601 cccagcaggc gcagcggatc gccggagacc gtgcgcacat ccggcgcggg ggagagcatg
    30661 ccgcggagcc acacgccggg tggcgcttga ttgaccgtgt ccatcgtcgg tgcctgcctg
    30721 ttcgattcac ggaatggtag cgacacgtgg caggaaagtc ttgagcccgg acaaggtgcc
    30781 ggtatcccgc cgcccggggg cgggcgcgag gacatccgtg gcgggccttt cgccatccag
    30841 tccggttatc tatgatggat ttcgggctga ccggctgcgg ggcttgcgcc ggacggcagc
    30901 actcaaaccc caacaggagc actcagaaca tgtacgacat cctgactctg gaacccgaag
    30961 acgacgtggt gatgcgggcg atcaaggcgg tcatcgcggg gcgggcaacc atcggcggcc
    31021 ctgagggtga ctaccgtctg ctgacaccgg gcgatacctg ggtggagggc gacggtggca
    31081 ccggcgtgga agtcctggtg atcgtcgggc acggtaacgc caacaagctc agtggctgcg
    31141 gcaagtggac gctcttccgg gaacacttcg acggccgggt ggactggaac cacaagacgg
    31201 aagtgtatct cgcggcctgt tcgaccgcag gtgaaggcgg ctccgccttc gtccatggcc
    31261 atttcgccgg cggggtgcgg gcggccttcc cgacccgggt ccgcgtgtgg gcgtcgacca
    31321 ccgcggttcg cgggcgggat ctcaccggcg actgggtgca gctctagtac gcgcggacac
    31381 ggtgcgtgcc ccggccggcc cggtcgggca cggaagcgaa gggcccgtgc agcgacagac
    31441 cgcctcctgg tctgcgacgc agggcgaccg cttcaggccg ggtgcgaggc gccgacctgc
    31501 gatccgcggc gctgcgccgg acgaaaggcc gctgtccgcg accgggaacg gacgcacgcg
    31561 gcggccaaac tctgctaccg tctgcagcca ctttcccttg ccggaggacg ccccgtgcgc
    31621 ctgaacctca gcctgtcgat cctcgccctc gccgtcgtgg cgggatgttc gtccccctcc
    31681 catgcctcca cccacgagcg tgccctcgtg acggccgaac tggtcgcgtc gcggccggcc
    31741 tgcgccggct tcatccgcca gttgcgggag accgtggcga ccgatcaggc gctcgccgaa
    31801 acctacgccg caatgcgggc ggccggctgc ctgcggcccg atgtctgatc cggcgggacg
    31861 ttgatcgcgt gcgtcggcac agcggccgcc ccgacggcga ccgaccggga ttgaccgatc
    31921 caggtgcccc tccatgaagg cgctgcatcg cagtgcgccg gtgcgggcgg tgactgcgcg
    31981 cccccggctc atcttcagca cgatctgtgg cgtgctcgcg ctcgtggcgt tacccgatac
    32041 ctgggcacat cagccggtat cccgcggcat ccttgcctgg aacatcggct gctggctgta
    32101 tctggccctg tccctgacga tgatgttccg ctcaacgcac gaacgcatgc agcgccgggc
    32161 gttggaccag gacgccgggc agcgtaccgt gctcgcgctg gtgctcctgg cggcggtgat
    32221 gagcctgggc gccatcgtgg ccgaactggc ggtggcgaag gatctgaccg gtccatccag
    32281 gctcctgcac atcgcctttg ccgccgtgac catcctgtcg tcctggttgc tcacccaggt
    32341 catgttcgcc cagcattacg cccatgactt ctatctggcc gaagcccgac agggcagcgg
    32401 cggcctcgcg tttccgggtg ggcacgcgcc ggattacggt gacttcctgt atttcgcctg
    32461 cgtcatcggc acctccggcc agaccgccga cgtgagtttc acgacccgcg ccatgcgccg
    32521 caccggtctt ttccactgtg tgctgtccta cttcttcaac gccaccgtgc tggccttgac
    32581 catcaacatc gcgtccggcc tggtgtagcg cgggcgtccg ccatgcgccg ccgtcatcgt
    32641 catgcaatgc ggatcttgaa gcatgcgtcg cgcagcatgt ccttccgcgt gcgcgcagac
    32701 cgcgcggccc gatacacttc ccttcctttc agatggagac tctgtcatgc ccatggatgt
    32761 cgtcgattac gaaatttttg gccacgagat gcagtacgtg gaggtggagc tcgatccggg
    32821 cgaggcggcc atcggcgagg ccggggtgat gatgtacatg caggacggca tcgccatgga
    32881 cacggtcttc ggtgatggtt cggcccagca gagcgggctg ttcggcaagc tgttgggggc
    32941 cggcaagcgt ctgctcaccg gcgagagcct gttcaccacg gtgttccaca acgaaggcac
    33001 gggcaagcgc cgggtggcgt ttgcggcgcc ctatcccggc aagatcgtgg cggtggatct
    33061 gtccacggtg ggcggcaccc tgatctgcca gaaggacgcc ttcctggcgg ccgccaaggg
    33121 cgtggctctg ggcatcgcct tccagcgcaa gatcggcgta ggcctgttcg gtggcgaagg
    33181 tttcgtgatg cagaagctgg acggtgatgg catggccttc atccacgcgg gcggcacgct
    33241 gctggagaag acccttgcgc cgggcgagcg cctgcgcgtg gacaccggct gcgtggtggc
    33301 cttccagccc agtgtggatt tcgacatcca gtacgcgggc aagatcaaga cggccctgtt
    33361 cggcggcgaa ggcctgttct tcgccaccct caccgggccc ggcaaggtat ggctgcagtc
    33421 cctgcccctg tcgcgcatgg ccgaccgcat cgtattggcg tcccgcgccg tgccgggcgg
    33481 cggcggtaag gaaggcggct cgctgctgag cggggtgggc ctgggctccc tgctcggcga
    33541 cgacgagtga gcacgcccgt cgcggcgcgt cattacccgc ccggcggcga cgcccacgga
    33601 cgcgccgtga ccctgaaggc cgccgtcggg cagctcgtcg tcgctccggt cgaaggcgcc
    33661 gcgctgcatg tgcccgcggc gcgcctgcag gcctcgccgc ggggcttcaa ccacagccag
    33721 tgggccctcg cgtgggccgg cgcggacggc gatcacctgc tcatcgtgga cgacgcggcg
    33781 gtcggccccc tgcgcaatgg gctgccgggg ctgttcgcga cgggcgatca ggcgcggcag
    33841 cgggccggtc tgcaccgtgt cttcgcctac ggggtcctga tcctcgcgtg cctggccctg
    33901 gtggtcggtg gcgtgtgggg cttcgacggg ctggtggatc gggtcgtggc gcacatcccc
    33961 gcgtccgtcg aggcgcagat cggcgaggcc gtgctggccc ggacgcgcca ggatgggccc
    34021 ttcatcgaaa cggggccggc ggcggaggcc gtgcaggcca tcggccggcg cctcacgcgg
    34081 cccggcgaaa ccctgcaatt ccacctggtg cagagtcccc aggtgaatgc cttcgcggcg
    34141 ccgggcgggg tggtcgtggt gtactcggcg ctgctgcgcc aggctggctc ggcggacgag
    34201 gtggccggcg tgctggccca cgagatcgcc cacgtggagc tgcgccacag cctgcgccag
    34261 ttggtgcgct cggccggcct gcaggtcatc gccgcggcgc tcttcggcga ttatgcgggg
    34321 ttggggcaac tgggcgccca gctcggcgcg ctcaagttct cccgcgacgc ggagcgggag
    34381 gccgacgccc gcgggctgca gcggctggcc gatgcgcaca tcgatccgca gggtctgctg
    34441 cgctttttcg aaaccctggc ggacacggaa ggcatggcgg cgacgatgcc cgccgtgctg
    34501 tccacccacc cggccacggc ggaacgcatc gacgccctca agcgcgtcct ggcggcccat
    34561 ccggctgaag cggtgacgcc gttggcggtg agctggccgg ccgtgcacgc tgccctggac
    34621 aagaccgccc ggtaagcccc gccgtcgcct cggcgcgccg ccccccgtgc ccgcccgcat
    34681 gcgcgtgcgg acatgggccg acgccctgaa gacggaggct ttcccgcgcc ttgtcccggg
    34741 attcgcaacg ggacgggctg cgcctgcggc ccggcgtgga catgcacccg gtggtgggcg
    34801 ccgtgcgggc cctgagccgt cggcactgac tgtgctgtca ccacacgggg ccgggcggac
    34861 cggcgatccg acacgcgatg ccgggctcag ggctgtcgcc gcgccccgtc ggtgtctggg
    34921 gcgggccgca ggcggaaccg accggggagg tcttgataca cccccaggcc cgccaggatc
    34981 agccccgtgc cgatccacac ccgcgggccc agcgcctcgc cgttcagccc gtgacccagc
    35041 agcagcgcca tcaccggcgt gatcagggtg atcagcgcca ccttggaggc gtccatgtgc
    35101 tggatcacgt agtagtacag cgcaaagccg atcaccgaac cgaagacacc caggtaggcg
    35161 atggcggcgc cggtgcgcgc gggcagctcg gccggcagat gcccgtcgcc cagccaccac
    35221 atgagcccga agccgggcag ggacacggcc agggtgccca ccgtggtggc caggggcggg
    35281 ctgtcgtcac cgatgcgctt gagggacacc aggctcgccg aatacacgca gaccgccgcc
    35341 agcagcgcgg cgaggcccgc caccgcatgc tcaccgccca cgctgccgcc atgcacgaag
    35401 atcaccgcca gacctgccgc gcccagcagc gtgcctgcca gcttggggcg ggtgagggcg
    35461 tcctcgccca gccagacact cgccagcccg ccggcgatga ggggcgacag gccgaaaagc
    35521 accgacacca gcccggaatt gacgaaacgc gcgccccagt aggtgagcgc catggccccg
    35581 aagagcccga tgccgccgga caggtaggcc cgccgcgcgc gggcatggaa gggcagggga
    35641 atgcgccaca gcgccatcaa cagcgccgcg acgctgacgc cgatgagcat gcgggcgaat
    35701 acggccaggg cgtaaccggc gccctgggtg ctccactgga tggccagggg cgtggtagac
    35761 cagatcagga cgatgctgag ataggcgagg gggacggaca tggggtgctc cggatcaaca
    35821 gggggacgag ggcggcggag aaggcggcac ggggacgcgc acgtccgggc gcagggccgg
    35881 agcgtggcgg gagatgcggg caaagccggc cgggcggcgg aaactcaggc ggatgcaggt
    35941 catgatcaga tctccacggt caggaaaaga gacgacagtc gggtaggggg gaaaatgaaa
    36001 caggccgcgg ggtgaagcgc ggcctgtcgg gaattcgggg cgttcgggac atcagtccgt
    36061 tcggcgcccg cccggccgcg gtgcacgcgc aaacgcgcgc agcgcacggc gcagtcggat
    36121 gatcggcagg aaggcggcgg gggatgccga ctggatgagg tccattgcgg tattctcccg
    36181 gtcgtttgtg cgtcgcgtca aggctggttc ggccaatgga tggggtggcg cggattttca
    36241 atggacaagg aggattcaca ggatggataa ggcacgattc gtggagatcg ttcgggacac
    36301 gccgctggtg tcggtggatc tggtggtgct ccgggaccat cgggaggtgc tgctcggcct
    36361 gcgcaccaac cggccggccc aaggcacctg gttcgtaccc ggcgggcgcg tcctcaagaa
    36421 cgagcggcgc gcggatgccc tgcagcgggt ggcgcagcgg gaactgggca ttgccgacct
    36481 ccacgccctc gcgccggaat tcctgggccc cttcgagcat ttctatccag actgcttcgc
    36541 cggcgacaac ggtgtgagca cccattacgt ggtgctcgct cacctgatcc acgtgcccgc
    36601 cggcttcgag atccccgggc aggacgatca gcacgaggcc ctgcgctggt ggcccctcga
    36661 tgaggcggac gcggagccgg cggtgcatcg cttcacgcgg gattacctgc cgctgctccg
    36721 ggcgcacgcg atgcgctgac gcggcatact ttcctgggtg ccgggtgggc ggacgttcgt
    36781 ggccatggat cggtacgaac agtcgggggc tgcgcagagc atcttcgcga cggtgtagcg
    36841 actacgcaag gccgcgatgt cgctacgcag accatggatg tgacattcgg tccgtgcgta
    36901 gtcgctccgc tgcgcaagaa tgtgactgct cgccggtgcg tagtcacttc gcagaccgtg
    36961 aatgggacat cgtgccggtg cgtagcgact ccgcaggcgg cggaacagac atcgcgcgcc
    37021 tgcggagtga ccatgccgcc cacggatgtg acatcccaca ggtgcgtagc gatgacgcac
    37081 gccatgcgcg tgagcacgca tgcagggcga gtcgcttcgg ccgatgaagg ggcgaccatc
    37141 aatagcagcg gaggcagctc gccgtggcgg cacggaggta tcgccagggc gtaaggcttt
    37201 ccgccagtcc cgatatgcgt gattcggctg ccgggtgacg tccaccgcan nnnnnnnnnn
    37261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gctttccgcc agtcccgata tgcgtgattc
    37381 ggctgccggg tgacgtccac cgcatccgcg ccggatgtgc aggcgcccgt gcatcccatg
    37441 ctcattccag tccaatcggc atctccccgt cagcagatcg aggcacctgg tcgcacaggt
    37501 attcttattt catgggatgg ccgtgctgca ggtggtaatg catccaatat gaatatcaac
    37561 aaaattgatg agtgcatata catatgtatt gaatgccagg gtgtgaagag gtaggatcgc
    37621 gctccgtaac accccgattg aatggcatac gcttacggag gagtcatgaa caacgtcagt
    37681 gaaagcaccc gcgtcagccc caagcaggtc cagaaagaca tcgagtccta ccacgcgctg
    37741 ggctccatgg ccgactacca tgccaacaac gccgaaaaca gccgcgaagc ggccgatacc
    37801 gcctatcagg ccctgcgcgc cgccgagcaa caggcggtca ccacccaagc ggctgccagc
    37861 gccgccgccg atgcgctggt ggtcgcccgc cgcaacttcc acggcgtcat cctgggcgtg
    37921 aagaacgaag cccgcgcgct gtacggaccc agttccgatc aggtggcggc gctggggctg
    37981 aagaagaagt ccgagcagag caagcgggcg cggagcaaga agacgccggc ggagtgaacg
    38041 tcaggggtct gcccgtcatg ggggacgggc agacaagaaa catgcacccc gctatccaag
    38101 cgaggtgcag tatcggttca accggcggca ggcttgggtg ccgagacggg cctgggccgc
    38161 gatgcacacc ccaccctgcg gaaagcgtgc actcattggg cgtcctgaag gtgaggcatc
    38221 atcaggtttc cgtgagtgtc ggcttccgat ggattccgtc tattcatcgg tcggcgagct
    38281 tgcctgcaaa aagcgccacc gcatcggcca gcacgcgggc gggggccgtc tggcggttcc
    38341 agtggtgcca gaacagcggc atgccgatcg gctcgcctgc ggccagctcg cacaataggc
    38401 cgcgctcaag cgcgcctctt gcctgcggca gggggaccag accgtacgcc agtccggcgc
    38461 gcacggcgcc aaagagcgcg gtcgaatccg gaatgacatg acagggataa tcgcccggct
    38521 gcaagcccca ggcctcgcgc agccagcgac gatgaatgct gtcctgctgg ccgtagacgg
    38581 cggcgggcgc atcggccaat gcatcggcgc ccagcccggc aggaaaacag cgggcgacaa
    38641 attccggtgt agctaccggc acatactgca tgaccccaag gggtatcacg ccacaaccgg
    38701 ctatctgctg gggctgtgtg ccaatacatc cgagtacctc cccggagcgc aactgatcga
    38761 gggtttgagc ctcgtccgcc accacacaat cgagcagaat ccgcgccgcc tgcaggctgg
    38821 gggcgattgc agcgataaga cccagcgcca ggctgtccgc gttcacgcca atgcgcagcg
    38881 tcagccagtc ggaggcagcc gtctccagcg tttcctgcag ttcggcttcc agcagccgta
    38941 cctgccgcac atgcgccagc agtttctgcc ctgccgcggt ggcgcgcacc ggtgtatcgc
    39001 gcaccagcag caccgttccg ctgcgctctt ccagctggcg gatgcgctgc gagattgccg
    39061 actgggtcag gtgcaggcgc cgcgccgcgc cttcgaaact gccggaggcc gcaacggcat
    39121 ccagcgcttc gagcaatttg taatcgagca ttagccagcc tgcatgccaa ttagaaaaga
    39181 tcgttttacc acggcgcggg gggtttgatt gaatagcgta atgacaagaa accgtgagga
    39241 aatgcgcatg aagcccgtca atggcccgca actggtgcag atcgcccgcg aatacgggac
    39301 gccgtgctgg atttacgacg ctgcaatcat ccgcgaacgc atcggcgagc tgcgccagtt
    39361 cgacgtgatc cgctttgcgc agaaggcctg ctcgaacacc cacatcctgc gtctgatgcg
    39421 cgaacagggt gtgacggtgg attccgtttc gctgggcgaa atcgagcgcg cgcttgctgc
    39481 cggtttttct gtgggtggtg tgcacagcga tatcgtgttg accgccgacc tgcttgatcg
    39541 tgccaccctg gcgcgcgtgg tcgaactgaa agtgccggtg aacgccggca gcccgcagat
    39601 gctggaacag ctcggccagg ccagtcccgg ccatccggta tggctgcgca tcaacccggg
    39661 cttcggccac ggccacagcc agaaaaccaa taccggcggc gaaaccagca agcacggcat
    39721 ctggcatgaa caactggatg aagcctacgc tctgatcgag cgctatggcc tcaatctggt
    39781 cggcctgcac atgcacatcg gctcgggcgt cgattaccgc catctcgccg aagtttgcga
    39841 cgcgatggtc gcccaggtga aggccagcgg ccgcgacctg caggcgattt cggccggcgg
    39901 cggcctgtcg attccgtaca ccgtcgaggg cgagcgcatc gataccgcgc actacttcag
    39961 cctgtgggac aaggcccgtt gcgaagtgga agcacacctc ggccacccgg tgacgctgga
    40021 aatcgaaccg ggccgcttcc ttgttgccga atccgccaag ctgatcgctg aggtgcgcgc
    40081 caagaaggac gccggcagca atcacttcgt attgtgcgac gccggtttca acgacctgat
    40141 gcgcccggtc atgtatggca gccatcacga aatcagcgtg ctgaagcccg acggcgcagc
    40201 ccgcacgacc gatcagcgcc cgaccgtcgt tgccggaccg ctgtgcgaat cgggtgacgt
    40261 gtttacgcag gaagaaggcg gaacggtcat tccacgcacc ttgccgacgg cggagatcgg
    40321 cgactggctg gttttccatg atgtcggtgc ctacggtgca tcgatgtcgt cgaactacaa
    40381 tacccgcccg ctgatcccgg aggtgctgat tgatggcggt gaaatcagac tcattcgccg
    40441 tcgtcagacg gtagccgaac tgctggcatt ggaaaactga tggacgccgc ccacgcctac
    40501 gagcaccacg cccacagctt ccttgccggc cgggacgcct ccgggatcgg tgcgcgggtc
    40561 atcagggact ggtgcgcgat gctcccgccg ggggccacgc tcatcgaact ggcctgcggg
    40621 ggcggttatc cggtgacgcg ggtgttgcgc gaggcggggc tgcgggtgtg ggcggtggac
    40681 agttccccca cgctgctggc gacgtttgcg cagcgcttcc cggagattcc ggtgcagtgc
    40741 gcgaaggtgc aggagagcga tttcttcggc cggaccttcg atgcggcgat tgcggtgggg
    40801 ctgatcttcc tgttgccgga ggcggagcag gtcgcgctga tcgcccgggt ggcgcagatc
    40861 ctcgaacctg ggggacgctt tctgttcatg gcgcccgtgg aacggggcga atggcaggat
    40921 ctgaacactg gcctgggctg ccgctcgctg gggcaggcgg cctacgaagg gcatctgtcg
    40981 gacgcgggct tccgcgtcgt cgccacgttt gtggacaagg gtgccaacca ctattacgac
    41041 gcgcagcggg tgcgctgagg ggctcggcgg gaagcgcgag cgcgctgggc ccaactccgt
    41101 cgtcgatggg ctgggtgcgc ggtgaaatct gccgcgcctt gcatggcttg ggcccaaccc
    41161 tcgcagtggt gacgggccga cgcgttgaaa tcccgatcat gcgacgggca gccgacgctt
    41221 tcacgggctg atacgaagcc cgctgcacgt atgttgcgat cccaaaaaag aagcggcccg
    41281 aaggccgctc aaacactgcg ttgcaacagg gttggggatt acttcttgcg ggccggcggc
    41341 acgtcggtgc aactgccgtg ggccacttcc gcggccatgc cgaccgtctc tcccagggtg
    41401 gggtgggggt ggatggtctt gccgatgtcg actgcgtcgg cgcccatctc gatggccagg
    41461 cacacttctc cgatcatgtc gccggccgag gggccgacga tggtgccgcc gatgacgcgg
    41521 tgcgtttcgg cgtcgaagat cagcttggtg aagccgtagt cggcaccatt ggcgatggcg
    41581 cggccgctgg cggcccacgg gaacttggcg gtgtccacct tcttgccttc ggccttggcc
    41641 tgggcttcgg tgtagccgac ccaggcgact tccgggtggg tgtaggcgac gccggggatc
    41701 acggtggcgt cgaaggccgc cttgtgaccc gcggccactt ccgccgccac atgggcttca
    41761 tgcaccgcct tgtgggcgag catgggctgg ccgaccacgt cgccgatggc gaagatgtgg
    41821 ggcacgttgg tgcgcatctg ggcatccacc ggg
//
LOCUS       DAOQPA010000014        41837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_372_length_41837_cov_21.082045, whole genome shotgun sequence.
ACCESSION   DAOQPA010000014 DAOQPA010000000
VERSION     DAOQPA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 41837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41837
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_41837_cov_21.082045"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1225..4587)
                     /locus_tag="PKX01_03490"
     CDS             complement(1225..4587)
                     /locus_tag="PKX01_03490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ75215.1"
                     /translation="MDLQRLVAQIDDALLAELFSANALAKARAYVGRVQHIEAAGHQI
                     QALVQGSDAAPYHVTVRIERREFFGKSSIELATRCTCPVGNRCKHAAALVLAARRAGA
                     LVDKPRAEILNWARSLGERLAKADKNRRKAAASEAIFYLCSAAQYGDDVEFGLLKGRV
                     GADGHPSGRASAWYNYDQALLKPPSFVRDEDLQVFRLLREVVRHGAGYGRPRLRGTEG
                     LALFEAALATGRAYLELDDNGRIQPLQAAAPRPGALIWAPDEAGLRPRVAVTPAAPLV
                     IRTEPLAWIDSATGEAGRVNIDGGGALLEVLALPPLSTTEIPVVAAALAQVAPALPRP
                     GAADATELPVLDVPCQPVMQLESLRCWSWRKHRSYPADFLGQDYDVAIPAFHYGDVRF
                     EPGSTHEIAALPDGRTVRIARDKTAEAAAWRAFQNAGFQPVKPGWLMIAGSTPAGLHG
                     LESEAHWTHFFSLLAPALRDAGWRIDCPADFRHRVLTPGEWFADISETDDGGFAVALG
                     VEVDGHRRDLAPMLHAVFRQDRRWLDRAQVDAIPDDAPVTILTEDGDRIVLPAGRLKP
                     IARTLVDLFDSPAGALKVSRLDAPRLAEAFADGWRAEGLASVDAWIERLRGAGGIAPV
                     PPPAGFAHDLRPYQREGLAWLQHLRQHRLGGILADDMGLGKTAQTLAHLLTEKHAGRL
                     DKPALVVLPTSLVFNWQQEAARFAPALRVLGLRGSSRADAFARIPEHDICLTTYPLLW
                     RDRDALSAHAYHSLILDEAQTVKNAASQAAQVVRGLRADHRLCLTGTPLENHLGELWS
                     QFDFLLPGFLGDAKDFTTRWRTPIEKHGDLIRRDILARRIAPFILRRRKEDVAKELPP
                     KTIVVRSVELDGRQRDLYETVRASMDQRVRDEIAARGFARSQIVILDALLKLRQVCCD
                     PRLLKTDAAAKVKERAKLDLLMDMLPELVEEGRRILLFSQFTAMLQLIAAELTARKIP
                     FVTLTGDTDDRAAPVRAFQEGSVPLFLISLKAGGVGLNLTAADTVIHYDPWWNPAVEN
                     QATDRAHRLGQTKAVFVYKLVVAGSIEEKILALQEKKAELAAGILAEDHEGSVKFGED
                     DLRALLAPLPETPREEADAAPKKRGRPKKTPG"
     gene            complement(4646..5452)
                     /locus_tag="PKX01_03495"
     CDS             complement(4646..5452)
                     /locus_tag="PKX01_03495"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HMZ75216.1"
                     /translation="MTFDTLNDHSSAIEAVGRLLSTARRVLFITGAGMSADSGLPTYR
                     GVGGLYEGTVTDIGLPIEKALSGAVFASRPDITWRYLAQIESACRSARPNAGHYAIAR
                     LEAQRGGVTVLTQNIDGFHRGAGSRDVIEIHGTLHRLRCVSCGRAREVADYAGLSIPP
                     ECPHCGGVLRPDVVLFGENLPEAAVQRFEAALAEGPDLVFSVGTGSAFPYIAGPVVWA
                     AQQGIPTVEINPGTTPVSDIVSMRLPLRAGEALPALLDAAGLGLEGIRPD"
     gene            5574..6392
                     /locus_tag="PKX01_03500"
     CDS             5574..6392
                     /locus_tag="PKX01_03500"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HMZ75217.1"
                     /translation="MCQLLGMNCNTPTDICFSFAGFQARGGLTDVHGDGWGIAFFEGR
                     GVRVFLDPAASCRSPVAELVRQYPIRSLNVIAHIRKATRGIVKLENTHPFQRELWGRY
                     WVFAHNGHLGEFDPPLRGDFRPVGQTDSERAFCFMLESLRTRFPDGEPARPALFAAIR
                     ELATAVSAHGEFNFLLSNGTCLFAHCASRLSYIVRQAPFAQAHLKDQDITVDFSELTT
                     PGDRVAVVATTPLTDDEVWTPIAPGSLMLFVDGAPEAAGEASTRAFIRTESVVA"
     gene            6500..6943
                     /locus_tag="PKX01_03505"
     CDS             6500..6943
                     /locus_tag="PKX01_03505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMZ75218.1"
                     /translation="MPHSAPKDCPLCFPERETVLWQDASCRVIRVDDADYPGYCRLIW
                     NTHVAEMSDLSTADQRHCFNILMAVEVALRECYRPDKINLASFGNVVPHLHWHLIPRY
                     TEDRHFPHPVWGQAQRRGGVSPAPAVDDAHLAGALRNAIAERLSG"
     gene            7044..8654
                     /locus_tag="PKX01_03510"
     CDS             7044..8654
                     /locus_tag="PKX01_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75219.1"
                     /translation="MSDNAVTFTELRSVEQYRKLLRNLHTLNASESHATLVRMLEGLL
                     ASPLGPEEQLEVLESARQTITFVQHEMSSRYAAHPLPPDSAEDATLRHVVRLWTALST
                     AYARLGDSGANDAEFRAQLPLLAQRRIQYLGQIIVEHYRAHRAEPPGAWRAVHAAFLE
                     AERAGITHSRVIDPLNNTWKAQSPNEAYIALLLIDLTNPYGRNQRELTWICRWAQRFA
                     PYCIMQTTWDKDDTRTYGLDPSEDHGLRPIALIGNRPGMRRFDGHRLAAQIKAMLAEF
                     KQGTTPASLGLGDDCAQPACARLLVSLYRPWGMSAAGRRFPRRPTRGVNRICGDWDAI
                     GMLVSGKEFVPPNRRQMMGSRYDMNMLTFGARADDDIEDIRDPEAEVQRRGHAIDEWQ
                     TVDHSVSGFRLMRRQPAQRIEHHQLVGIKPEDGGPYLLGQVSWLMFEADGTLMAGIHL
                     LSGLPTVVAARPVSVNVSAHEPYSQAFMLPAIPAMRAQATIVMPSGWFQANRVLDISP
                     GEIRQIRLTSLIARGTNFDQATYEVIGG"
     gene            complement(8660..9961)
                     /gene="phoR"
                     /locus_tag="PKX01_03515"
     CDS             complement(8660..9961)
                     /gene="phoR"
                     /locus_tag="PKX01_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HMZ75220.1"
                     /translation="MVVGVLVVLAVIFVLGLGVGLFFGPVPGLTVFSVALLAAGVYHI
                     GNLFKLVKWARQPVGTPLPRAVGLWDFVYADLNRLIRQAQDQRAKLTAALARFREASQ
                     AMPHGMMFLTDTNFIEFANRQAELHFGIDNARDKGAPVINLVRQPDFVRYMNEGRFDE
                     PLTFSQFRPQSRNLMVHVVPFGEGMRLLMSEDITQGERLETMRRDFVANVSHEMRTPL
                     TVVRGFLETVLDGLDDLDKDDIRHYLAMAGEQAGRMGRLIEDLLALSALETGAPMPQE
                     EPVDIARMLKDVEQEARAVSAERHSIVVDPPAPGTERILGNSKELHSAFANLAVNAVR
                     YTPAGGHIHIAWRLRGAVVEYSVEDDGIGIAPEHIPRLTERFYRVDRGRSRETGGTGL
                     GLAIVKHVLSRHQADLHIDSEPGRGSRFSACFPARRLAAAE"
     gene            complement(10028..10738)
                     /gene="phoB"
                     /locus_tag="PKX01_03520"
     CDS             complement(10028..10738)
                     /gene="phoB"
                     /locus_tag="PKX01_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HMZ75221.1"
                     /translation="MPATILLVEDEPAIQELIAANLLRAGHQVVRAGDAETAQRIVRD
                     ALPDLVLLDWMLPGMSGIDFARRLRADERTRGIPLIMLTARGEEQDKVLGLEVGADDY
                     VTKPFSPRELMARIKAVLRRRAPESTEDPVDLGGLRLDPATHRLTAGSTPVSLGPTEF
                     RLLHFLMTHPERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPTHHDGLVQTV
                     RGSGYRFSTQTESQPAVR"
     gene            10903..11328
                     /locus_tag="PKX01_03525"
     CDS             10903..11328
                     /locus_tag="PKX01_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HMZ75222.1"
                     /translation="MAGLQKGDPLPTEIKLHQRSRILEITFDDGQRFELSYEFLRVHS
                     PSAEVRGHGPGEEVLQLGKRDVEIVNVEPVGNYAIRPIFSDGHDSGLYSWDYLYEIGE
                     RRDELWQTYLTRLAVEGGSRDPAELPPAAPKAGGCGHKH"
     gene            11390..12127
                     /gene="ubiE"
                     /locus_tag="PKX01_03530"
     CDS             11390..12127
                     /gene="ubiE"
                     /locus_tag="PKX01_03530"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMZ75223.1"
                     /translation="MNDKTTHFGFQKVAEAEKAKKVAGVFSSVASSYDVMNDLMSFGL
                     HRLWKAFTVQIAGLRAGDRVLDVAGGTADLSLAFAKKVGPTGQVWLTDINHAMLSRGR
                     DRVVDNGFLLPVAQCDAEKLPFPDDYFDCVTVAFGLRNMTHKDQAIAEMRRVLRPGGR
                     LLVLEFSKVWKPLAPAYDFYSFKLLPLMGEKVARDGESYRYLAESIRMHPDQESLKDL
                     MEQAGLGRVEYFNMTAGVVALHRGYKL"
     gene            12155..13042
                     /locus_tag="PKX01_03535"
     CDS             12155..13042
                     /locus_tag="PKX01_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HMZ75224.1"
                     /translation="MKKLAVAALMAVATLTTVVPDAEAARLGGGKSFGMQRNDTIVRR
                     EAAPAPQVPPRTAAAPAQSAPQAGAPAPQMPQKRSWLGPLAGLAAGIGLAALLSHFGL
                     GEQAASFMLILLGVMAVMMLFRLLFRRGASQPTPQYAGMPSTPAPETRHAASAPIALP
                     TSGAPVADTEALPSGFDADAFVREAKLNFIRLQAANDAGNLDDLREFTSPEMFAEIRL
                     QYDERHGKPQRTDIVNLNAAVLDVATEGSRHVVSTRFYGMLREEDDAAPTAFDEVWHL
                     VKPVDGSRGWMVAGIQQRH"
     gene            13107..13685
                     /locus_tag="PKX01_03540"
     CDS             13107..13685
                     /locus_tag="PKX01_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMZ75225.1"
                     /translation="MLTSTVIAALNHLLTDAPWARDRLKPFAGRTAVIQVAPASLCVA
                     VEGDGFFSDSATADTPDVRLELPVSSLPKAVGGMDALMGDIRITGNADFADALGFVLR
                     HLRWDGEEALAKRVGDIAAHRAINGVRDATRWHLDAARNLTENFVEYFTEERPLILAR
                     PQFDPFATEMAALRDDLARLDKRLSRLEAARR"
     gene            complement(13965..14390)
                     /locus_tag="PKX01_03545"
     CDS             complement(13965..14390)
                     /locus_tag="PKX01_03545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HMZ75226.1"
                     /translation="MAMTIHVDIVSAEEQIFSGLAEFVVLPGEAGELGIMPGHMPLMT
                     RIKPGAVRVKVQNQADEELVFVAGGLLEVQPNLVTVLADTAIRGKDLDEAKALEAKRR
                     AEEAMQNQDSALEYAKAQSELASALAQIAAIQKLRKRSH"
     gene            complement(14454..15830)
                     /gene="atpD"
                     /locus_tag="PKX01_03550"
     CDS             complement(14454..15830)
                     /gene="atpD"
                     /locus_tag="PKX01_03550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ75227.1"
                     /translation="MSQGTIVQCIGAVVDVQFPRDAMPKVYDALTLEGAELVLEVQQQ
                     LGDGVVRTIALGSSDGLRRGMVVSNTGKGIAVPVGHGTLGRIMDVLGRPIDEAGPIEC
                     DERRVIHQKAPKFDELSPSVDLLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKQHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVFGQMNEPPGNRLRV
                     ALTGLTMAERFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGR
                     LQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDSTVVLSRDIAALGIYPAVD
                     PLDSTSRQLDPLVVGEEHYNTARGVQQTLQRYKELRDIIAILGMDELAPEDKLLVARA
                     RKIQRFLSQPFFVAEVFTGSPGKYVPLKETIKGFKTILSGEVDHLPEQAFYMVGPIEE
                     VFEKAKTL"
     gene            complement(15858..16727)
                     /gene="atpG"
                     /locus_tag="PKX01_03555"
     CDS             complement(15858..16727)
                     /gene="atpG"
                     /locus_tag="PKX01_03555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HMZ75228.1"
                     /translation="MASGKEIRNKIKSVQNTKKITKAMEMVAASKMRKAQDRMRAARP
                     YAEKIRRLAANLSAANITDYKHPFLTKKDDAKRVGLIVVTTDKGLCGGLNTNTLRVAL
                     SALKSWEGNGVTDIRVTCIGNKGLGFMQRLGAKVISNVTQLGDTPHLEKLIGPVKVMI
                     DAFQNGELDEVHIAYSRFINTMKQEPQLEQLLPLTGERLGTPEGTWDYLYEPDAQVVI
                     DELLVRYVEALVYQGVAENMASEQSARMVAMKAASDNAGNVISELQLVYNKTRQAAIT
                     KELSEIVGGAAAV"
     gene            complement(16749..18287)
                     /gene="atpA"
                     /locus_tag="PKX01_03560"
     CDS             complement(16749..18287)
                     /gene="atpA"
                     /locus_tag="PKX01_03560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ75229.1"
                     /translation="MQLNPSEISDLIKSRIQNLQLSANARTEGTVVSVTDGICRIHGL
                     SDAMQGEMLEFPGNVFGLAMNLERDSVGAVVLGEYEGISEGDTVKCTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPINAKLSEPIEKVAPGVIWRKSVSQPVQTGLKSVDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGQDMYCVYVAIGQKASTVANVVRKLEENGAM
                     EYTIVVAATASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSAEYVEKYTNGEVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELMKQPQYSPLKVSEMAITLFA
                     VDKGAFDDVEVKRALACEAAMHQYVKTKAPDLINKIETTKDLDADASKQLADVIAEFK
                     KSWA"
     gene            complement(18299..18835)
                     /locus_tag="PKX01_03565"
     CDS             complement(18299..18835)
                     /locus_tag="PKX01_03565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HMZ75230.1"
                     /translation="MAENVTIARPYAEAAFQLASAGNALGPWSEALDRLAGIAADPQM
                     RDCISDPKLQPQQLAGLFLDVAGSGLTAEQQNYVRVLVDNERLQVLPEIRDLFVALKN
                     EHEGVKEANIVSAFPMDEATLKALVADLEARFKARLNVSVSVDPELIGGVKIAVGDEV
                     IDASVRGKLANMAAALKN"
     gene            complement(18839..19312)
                     /locus_tag="PKX01_03570"
     CDS             complement(18839..19312)
                     /locus_tag="PKX01_03570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMZ75231.1"
                     /translation="MNLNATLVAQLVVFFILAWFTMKFVWPPIVKALDERAKKIADGL
                     AAADKAKSDLAHAEKKVVEELRKARESATDVRAAAEKQAASFLDEARAEAARIIAQAR
                     EAAEAEAGVAAQRAKEALRDQVAHLAVAGAEKILRKEINAQAHAELLANLKQELQ"
     gene            complement(19347..19592)
                     /gene="atpE"
                     /locus_tag="PKX01_03575"
     CDS             complement(19347..19592)
                     /gene="atpE"
                     /locus_tag="PKX01_03575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HMZ75232.1"
                     /translation="MEHVLGFVALAAGLIIGLGAIGACIGIGIMGSKYLEASARQPEL
                     MNALQTKMFLLAGLIDAAFLIGVGIAMMFAFANPFKI"
     gene            complement(19655..20497)
                     /gene="atpB"
                     /locus_tag="PKX01_03580"
     CDS             complement(19655..20497)
                     /gene="atpB"
                     /locus_tag="PKX01_03580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMZ75233.1"
                     /translation="MSTGHEAHAPTAGEYIVHHLTHFNSTGHAQSAVIDWSVWNLDTL
                     FFSVGLGIVTLLILYKAASKATSGVPGRFQAAIEILVEMVADQAKGIVHSAESRKFVA
                     PVALTVFFWIFLMNSMDFLPVDLLPKIWAIMCGDEHAFLRVVPTADLNGTLGMSIGVL
                     LICLYYNIKIKGFGGWVHELFTAPFGSHPLLYPINFLMQMIEFLAKTVSHGMRLFGNM
                     YAGELVFMLIALMGGTATVFGFLGHVVAGSIWAIFHILIVALQAFIFMMLTLVYIGQA
                     HEGH"
     gene            complement(20500..20841)
                     /locus_tag="PKX01_03585"
     CDS             complement(20500..20841)
                     /locus_tag="PKX01_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HMZ75234.1"
                     /translation="MFKAVTLQIGATILAALIAGAFLGLRGALSAALGGAACFLPNLL
                     FALRLHLVTKRPDASYPVAFFVGEFFKIASTVGLLATVRLVYPDVHWLALFGGLVVAL
                     KANLFAFLVKT"
     gene            21027..21338
                     /locus_tag="PKX01_03590"
     CDS             21027..21338
                     /locus_tag="PKX01_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ75235.1"
                     /translation="MNKDYSRAIPAAWRDVSAVFAALGDEHRQRILLTFEAGERLNVG
                     QIAEVSTLARSTVSHHLKVLREAGVLSAQRQGKEIYFSVNTHFLQKAFARVSDYLETR
                     L"
     gene            21713..22546
                     /gene="rhlP"
                     /locus_tag="PKX01_03595"
     CDS             21713..22546
                     /gene="rhlP"
                     /locus_tag="PKX01_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922374.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HMZ75236.1"
                     /translation="MRPFPSALVLSLAASVSIAGCATWFNPAPHTQRATVVEYLYPKG
                     EQPTLTPSIPELHLPLRVGIAFVPSERFDDLPETERDRLLNRVRDAFANRPYIAAIEI
                     IPTAYLRPRGGFDNLEQAARMFNVDVVTLLSYDQVQFSDSNRLSVFYWTLIGGYIFNG
                     SQYDVSTLVDASVFDVKSRSLLFRAPGTSQITGSSPLVNFAEASREARSEAYRRAIDV
                     LIPRLDAQLEAFKVRVKDRQVARIVEKPGYSGGGALDPVALGLLGTGALLALAWRRRS
                     A"
     gene            22596..23123
                     /gene="rrtA"
                     /locus_tag="PKX01_03600"
     CDS             22596..23123
                     /gene="rrtA"
                     /locus_tag="PKX01_03600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HMZ75237.1"
                     /translation="MLVTLLPADAVAWRRDALGDGAWWRLWTGPFSHWSALHLAGNLT
                     AAAALSVLIGPPVWRWIAVLPAVAPLLSLFLIASAPDLQHYRGLSGLLGVLVVGAGVE
                     GGVIGRLVAGLYVVKLAVDAIEGASSPVLPTGVAVVWQAHLGGLLLGLLVAASFHLHR
                     RASASTPTRTPSDTP"
     gene            23120..25000
                     /locus_tag="PKX01_03605"
     CDS             23120..25000
                     /locus_tag="PKX01_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ75238.1"
                     /translation="MSTQFHLLRERRFLPFFVTQFLGAFNDNLFKNALVVVLTFQATR
                     WTTIRPELLANLAAGLFILPYFLFSASAGQLADKYDKARLTRLVKLLEMLIMGVALAG
                     FLLHTLMVLFVALFLLGCHSTLFGPVKYALLPQHLREDELVGGNALVEAGTFVAILLG
                     TLAGGLLAGMDSAERWIAVAGFLVAATGYLSSRAIPAAPPPAPTLRIHPNPFTETWRN
                     IGFARQNRPVFLAILGISWFWLYGALFLAQFPAFAKNVLGGGEGTVTLLLAVFTVGIG
                     LGSLLCERLSGGHLEIGLVPFGSIGLTLFGLDLALAAPAGVPAAAPLALSGVLAQPST
                     LRLLVALLMLGIFGGFFIVPLYAMMQSRSAVGHRARVIAANNILNALFMVVGALGAAA
                     LLAAGLSIPALFAAAAVMNAAVALFIYRLVPEFLLRFIIWLLVHSVYRLRTEGLPHLP
                     SEGPAVLVCNHVSFVDALVIAAACRRPIRFVMDHRIFRWPVLSFVFRHSRAIPIAPAR
                     EDTAMMEAAFDQVAAALAEGELVAIFPEGQITRDGELNAFRPGIRRILAASPVPVIPM
                     ALRGLWGSMFSRKDGPALSRPLRRGLFNAIELAVGAPLSPESATVEGLRARVLALRGE
                     RR"
     gene            25031..25261
                     /locus_tag="PKX01_03610"
     CDS             25031..25261
                     /locus_tag="PKX01_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75239.1"
                     /translation="MSYLITLIQAFVLLILLCLILAGGVCVASFPTEPAAVVVGLISM
                     LTFSWPAWLLIRTFRSRPAPKTEPERSDPPTP"
     gene            25258..26022
                     /locus_tag="PKX01_03615"
     CDS             25258..26022
                     /locus_tag="PKX01_03615"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ75240.1"
                     /translation="MIADPATAHALHAVFEALALFGGARLYVHQSQRQSRHSVFQGGR
                     FAVLCGCLLGAAIGNKVVFWLEMPQLFQQADGWQRIFAGQSVVGGLLGGLIGTEIAKA
                     LSGETASTGDGFVVPVLAGIAVGRVGCFLAGLHDGTFGRPTALPWGIDFGDGIPRHPT
                     QLYEILFVVTLAGLLQQLSPRLGAQPGLRFKLMLSAYLLWRLLIDGLKPLPYAWPFGL
                     SGIQWVCTLALSIYLPLTIRQWRQRHVTQEPPLPVL"
     gene            25988..27433
                     /locus_tag="PKX01_03620"
     CDS             25988..27433
                     /locus_tag="PKX01_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ75241.1"
                     /translation="MSRKNRPYLFYDTTASVCSTCLHRVEAKVLIKDDRVYLEKWCPA
                     HGRERVLIADDADYYRLAREVYVKRPEMPEHFSTTMTWGCPYDCGLCPDHMQHSCLTV
                     LEITEHCNLKCPVCYADSGPERQLHRSLGEIEAMLDAVVRNEGEPDVVQLSGGEPTLH
                     PDFFAILDAARARPIRHLMINTNGLRIARDPEFVARLAEYGRGLEIYLQFDSLRAEAL
                     HTLRGADLTRMHAQALEALEHHGLSTTLVMTLRNGVNDQEIGDVIRHGLGYRCVRGVT
                     LQPVQDAGRNLGGRGAEDRLTVSGIRRRIAEQSGLFTLDDIVPVPCNPDTLAMGYALR
                     LDGQVHPLTRWLGPEALLAGPANTIVFERDPSIRDRLFRLFSTNHSPASGARCLSDLL
                     CCLPRIDAPTLDYTNVFRILIVQFMDATNLDLRALKKSCVHMVQPDGRMIPFEAFNLF
                     YRGRLTEGLETLRAGVADDIARRHRSVPSAG"
     gene            complement(27422..28186)
                     /locus_tag="PKX01_03625"
     CDS             complement(27422..28186)
                     /locus_tag="PKX01_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HMZ75242.1"
                     /translation="MRARLLMAAALLLCVVPVGAELIHGSAIYRERLRLPPEAVLEVT
                     VEDVSRADAPAPVIGRTRLEPAGAPPIPFAVEVDPADVEAHRRYSLRGRISVGGAPWF
                     VTDQVYPVLDGGPDGAVTLLLRRVSSRPPVAGDSGGLRGAPWRLITQDGFPVDPQRSV
                     RIPQLHFLEAGRLAGTGGCNRFIGTYRVDGDHLRVSAHAGTKMACGQGMDAEVGFHAL
                     LDQVTHWRIEGATLLLLDDAGRLVLSLERTGSALAR"
     gene            complement(28263..28979)
                     /locus_tag="PKX01_03630"
     CDS             complement(28263..28979)
                     /locus_tag="PKX01_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HMZ75243.1"
                     /translation="MDSRSTFDSPSGFNRRQFLGASLGAVSALALGGFVRPAQAASPI
                     VLPALPYADSALAPVISANTLGFHYGKHHKAYVDNLNKLVAGSELADLSLEQIIAKVM
                     GKADKTAIFNNAAQAWNHTFYWYSLRPNGGGEPPAALKARIEASFGSVDALKKELAQA
                     ALTQFGSGWAWLVADGKALKVVKTGNADMPQAQGYTPLLTLDVWEHAYYLDYQNRRGD
                     YVNAVLDKLINWEFALKNLG"
     gene            complement(29084..29629)
                     /locus_tag="PKX01_03635"
     CDS             complement(29084..29629)
                     /locus_tag="PKX01_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HMZ75244.1"
                     /translation="MALKSTIFRASLTVSDLDRGHFGEHVLTLARHPSETDERMMVRV
                     LAFALYADERLQFGRGLSSEDEPAAWLKDDTGRILEWIEVGLPDEKLLRRASGRAEQV
                     RVLAYGGRSVDIWWQKDSAALARLGNLTVQALETDDSRALAALAERSMTLTCTLQDGQ
                     IWFSSGDTTLGFSLRTLHAPT"
     gene            complement(29705..30076)
                     /locus_tag="PKX01_03640"
     CDS             complement(29705..30076)
                     /locus_tag="PKX01_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75245.1"
                     /translation="MTTRALALAALIALGLAACSKKEEAPAPTPAPVVDAAKEAAQKA
                     EEAAKQAAEATKDAAKDAAGAAKEAGTAAAGAAKDAAKDAVAAAKEAAGAAKEAGKEA
                     VAATADATKEAADKAKAAVGK"
     gene            complement(30235..30795)
                     /gene="efp"
                     /locus_tag="PKX01_03645"
     CDS             complement(30235..30795)
                     /gene="efp"
                     /locus_tag="PKX01_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMZ75246.1"
                     /translation="MKTAQELRSGNVIMVGNDPLVVQKAEYNKSGRNAAVVKMKLKNL
                     LTGAPSENVYKADDKFEVVVLERKEVTYSYFADPMYVFMDADYEQFEVEAENMTDALK
                     YLVDGMPCEVVFYNGKAISVDLPTSVVREVEYTEPAVKGDTSGKVLKPARIAPSGFEL
                     SVPAFVEIGDKIEIDTRTDEYRNRVK"
     gene            complement(30852..32012)
                     /gene="earP"
                     /locus_tag="PKX01_03650"
     CDS             complement(30852..32012)
                     /gene="earP"
                     /locus_tag="PKX01_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HMZ75247.1"
                     /translation="MNNSTPGSSSDARPCWDIFCPVVDNFGDIGVCWRLARELAVAHG
                     VQPRLWVDDLPSARRLIPSLEPVLAIQHRAGVEIRAWTDPLPAIVPGDVVIGAFACSL
                     PAGFLEAMALRLPRPLWINLEYLSAEDWVAGCHRLPSPHPRLPLTQYFYFPGFDASSG
                     GLLRERDYDVRRAAHAPVQCLTGLGLPAARPGELRISLFAYENAALPELLEVWRNGTR
                     PLTCLVPEGRVLPQVAQCLGRNGLKPGDRIEAGALRVVVIPFVEQSRYDELLWACDLN
                     FVRGEDSFVRAQWAERPFVWQIYRQDDDAHLDKLDAFLARYAERLPPSAGAAVRDFWR
                     AWNRGHDAARAWQAFADALPALSAAAPAWSAHLQQAGELAAGLVQFSKDQLK"
     gene            32310..32579
                     /locus_tag="PKX01_03655"
     CDS             32310..32579
                     /locus_tag="PKX01_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75248.1"
                     /translation="MLSITDLLDFIDLDRDTVLIVAEATGLPPEPAIILARELLASRR
                     GILTLHHMFRDERARADERGHVLRVRDLDRAYRHFARKYPQPVVV"
     gene            32910..33821
                     /locus_tag="PKX01_03660"
     CDS             32910..33821
                     /locus_tag="PKX01_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ75249.1"
                     /translation="MSAPSSPISRAVRHPYLLLTLTVLFWSGNMVVGRALREAIPPMS
                     LALGRWILAFLLTLPFAWPHRNRLQTLDRREWGILAVLGVLGVGAYNTLAYIALSHTT
                     ATNAALLNSFIPVAILVLTWWLGHRSRPLEIIGVGVSLAGVMTILSHGELRVLTHLQL
                     NIGDVWMLGAVLSWALYTVCLRWRPEGLHPMALLAVLTAIGLMVLTPLVAWELAGGAQ
                     IRPDAGLLAGIAYTGIFPAFLGYVFYNRAVAQVGAATGGLFIHLMPAFSTLLAMVFLG
                     EQPHLHHFFGIALILAGIGLTTGFKNR"
     gene            33852..34322
                     /locus_tag="PKX01_03665"
     CDS             33852..34322
                     /locus_tag="PKX01_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ75250.1"
                     /translation="MRFEHLVEINDPLNPLIPSLTREQVWSGLMLRVDEPTVFLPGLE
                     RCEILSRAADQVERRLHFGGVQVRDTVTFSPLEWVRFESSANAEHAGGRLTIRLEQPD
                     PLRLYLRFTYETTLPDGGAADGVQVSEFVKSAYRESDVDTVRIIRVLSAGGRPH"
     gene            34371..35498
                     /locus_tag="PKX01_03670"
     CDS             34371..35498
                     /locus_tag="PKX01_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HMZ75251.1"
                     /translation="MIARPELRSFQPPVRTLMGPGPSDVPPRVLAALARPTVGHLDPA
                     FVGMMDELKELLRYTFQTRNELTFPVSAPGSVGMETCVVNLVSPGDTVVVCINGVFGQ
                     RLAENVRRAGGVPVIVEDPWGEPVDPAKVEDTFKAHPEASLLAFVHAETSTGALSDAA
                     ALAAIARRFGALTLMDCVTSLGGVPVLLDEWGIDAAYSGSQKCLSCTPGLSPVSFSEA
                     AVARIRARKTPCQSWFQDLSLVLGYWSGAKRTYHHTAPVNPLYGLHESLLMLAEEGLE
                     AAWQRHRDNHLRLRAGLEALGLRFVVREDARLPQLNTVWVPDGVDEAAARTRLLKEYG
                     LEIGAGLGVFAGKVWRIGLMGHSSRPANIALCLAALKDVLG"
     gene            complement(35588..35914)
                     /locus_tag="PKX01_03675"
     CDS             complement(35588..35914)
                     /locus_tag="PKX01_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75252.1"
                     /translation="MESDSVLYGLLGRIHLLMRRVTNRITDVEYMRTNKDYAREIVRL
                     ADASGHDELRELAGRLRDAMDLDAAEADTPSTPAPAPRNGFFERLRSPAVSPEGDKDR
                     YVRSLR"
     gene            complement(35938..37182)
                     /locus_tag="PKX01_03680"
     CDS             complement(35938..37182)
                     /locus_tag="PKX01_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ75253.1"
                     /translation="MAALLALSGPALAQSAPAAHAEGGNTAWMLTATALVLLMSLPGV
                     ALFYGGMVRSKNVLSTMTQTIAIAAVVTLTWMVAGYSLALRPGTPFLGGLDRLFLEGL
                     QAEGLAGGAVPETVYVMFQLTFAIITTALVPGAFAERMKFSAVLLFAALWSVLVYAPV
                     AHWVWEPGGWLAALGALDYAGGTVVHINAGVAGLVCALVLGPRQGFGREPMTPHNLTL
                     TLSGAALLWVGWYGFNAGSALAADSHAGMAMLVTQMAAATAAVTWMAVEWMLRGRPSA
                     LGLVSGAVGGLVAITPAAGYVSPSSALLIGLAAGAGCYIASTSLKRMLGYDDALDAFG
                     IHGVGGIIGALLTGLLASPAIGGSAGRMSAQLAGVGATVAYSALMTLIVLGIVQVITG
                     LRVDATTEADGLDIAEHGEALD"
     gene            complement(37503..38879)
                     /locus_tag="PKX01_03685"
     CDS             complement(37503..38879)
                     /locus_tag="PKX01_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ75254.1"
                     /translation="MQYSSQSVAKPYFIAAIGLFLGQILFGLIMGLQYVIGDFLFPAI
                     PFNVARMVHTNLLIVWLLFGFMGAAYYMIPEETETELYSPKLALLMFWVFLVAGAATI
                     LGYLLVPYATLAKFTGNDILETMGREFLEQPLLTKIGIVVVALVFLFNLTMTMLKGRK
                     TSIGIVLMLGLWGLAVFFLFSFYNPHNLVLDKFYWWWVVHLWVEGVWELILGSLLAFV
                     LIKVTGVDREVIEKWLYVIITLTLVTGIIGTGHHYFWIGLPEYWQWWGSVFSALEPIP
                     FFAMTVFAFNMVNRRRREHPNKAATLWALGTGVMAFLGAGVWGFLHTLAPVNYYTHGT
                     QITAAHGHMAFYGAYAMVVITIISYAMPILRGRAANSEKAQTMEMWSFWLMTVAMVFI
                     TLFLTAAGILQVWLQRFSSSPIPFMAAQEQISLFYWLREGTGVVFLVGLLIYVASFFV
                     SGEEKAAA"
     gene            complement(38908..39333)
                     /locus_tag="PKX01_03690"
     CDS             complement(38908..39333)
                     /locus_tag="PKX01_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ75255.1"
                     /translation="MSGTFTKSMARNIFFGGTVFFALLFIALTFDSMQTLPKRDNRAN
                     LTPAVAAGKKVWEVNNCIGCHTLLGEGAYFAPELGNVYKRRGPEFIKAWIQSQPTGAP
                     GRRQMPNFHLTDQQLNDLVEFLKYTSEINTANWPPNIEG"
     gene            complement(39485..40018)
                     /locus_tag="PKX01_03695"
     CDS             complement(39485..40018)
                     /locus_tag="PKX01_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75256.1"
                     /translation="MSEDALPPLLLSLSGGDRRSIGRVADAVEAVLTDPALLATLFEG
                     MQRPDPVLRMRCADAAEKISARQPQWLLPFRDQLLDGLAADPQQEIRWHVAPMLLRLP
                     LSTAEADRVYAILVGYTADPSRIVRTEAMQALADLARRHPRFTDEVRRLLTALAASGT
                     PAMQARGRRLLARLNAA"
     gene            complement(40090..40971)
                     /gene="cobA"
                     /locus_tag="PKX01_03700"
     CDS             complement(40090..40971)
                     /gene="cobA"
                     /locus_tag="PKX01_03700"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMZ75257.1"
                     /translation="MPPPVTAFALSDAGALRAFLDALVHRHAGGRHDAARPAAAGRPA
                     GRVALVGAGPGDPELLTLRAARLIAAADVLVVDHLVGDGVLALAHPGAERFYVGKEAG
                     HHTLPQEQINQLLVRLAREGRHVVRLKGGDPFIFGRGGEELEELLAAGIPFEVVPGIT
                     AACGIAAYAGIPLTHRDHAQSVVFATGHRRAGESSLDYVALSRPHQTAVIYMGVGMLL
                     SLCGALIAHGRDPATPAALIEKGTLPEQRVVTGTLATLPDLANLAGVRPPALLIVGEV
                     VSLAGKLAWTPSACSSV"
     gene            41126..>41837
                     /locus_tag="PKX01_03705"
     CDS             41126..>41837
                     /locus_tag="PKX01_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HMZ75258.1"
                     /translation="MKQLWKTGAMALALLPLAVSHAFAEDAKTTTNAPEAKYQSAASP
                     LANVEMYQDINPKAPPMSKAEFDKARQIYFERCAGCHGVLRKGATGKPLTPEKTLAAG
                     TDYLKVFIKYGSPAGMPNWGTSGELSDDEVDLMARYVQQTPPQPPEFGLKDMKATWKV
                     IVPPEQRPKKKMNNYNIENIFSTTLRDTGEVALIDGDTKKIINIVKTGYAVHISRVSA
                     SGRYLFVIGRDAKINMIDL"
BASE COUNT         7164 a        13540 c        13781 g         7352 t
ORIGIN      
        1 tcggcagaga aaatattgac cgctttcgag caataaggac atttcatgat tggtgagttc
       61 taacgtaacg tagacgacat ctgacgccct tggcgtctta cacgactgtc gcctaagctc
      121 ccaggatgtg ccaagtgact gtatttaagc ataatcgatc tagagctttc cgtcatgttg
      181 tcgtctaccc ttcctgaagt gtttcctgca ttctctcgtc tgctgaatca ggttcgacag
      241 cgaatcagca tccagcctga aagcggatcg tgcccacacg ggatgaatta aacgccacat
      301 ccaccctcat gacaagcggt acatgcagaa ttaggcgata caggcattga cggatgtcgg
      361 ggcgcacaca taatcgagcg caagctttat gttttagcgc cgcgctcagg cgcggtgggc
      421 tgtgttcttt ctatccgtaa agtgattggc agtgagaatg gcgatctggt gactcaccag
      481 gaaacgccga cgcaggcttt caatactgat tcgcacgggg ccatcgtttt ggcctggtgc
      541 aaccgaagcc tacgctcacc gcatcaagca cgccccccga cattccccct ccccgcatcc
      601 cgatcatccg ttcccccacc tgcggagtga cttcccggcc gtgcggagtc actttcgccg
      661 cgtgcgtagt cactttcgct gcgtgcgtag tcactctcgc cgcgtgcaaa gtgactacgc
      721 cgccgcgctt agtgacatct cgcggccgcg aagtgcatac gcagccccag attgtcacta
      781 tcgcgacgtg gacacgcact gcgcacggga cgacagtcac tgcgccgccg cgatatgtgc
      841 ctttccaccc ggcagatgtg tgttttcccg cccgagatcg atctgcgccg atgcgctcag
      901 acgctgtcga cgtcgacaac aaagctgttg atagctaaaa acctttgttg caggcctgct
      961 aatgatggaa agggctatca atcctgtgtg tcgggggcgg cgacaaggct gttgacgggt
     1021 cgacatcgtc tgcgcagggg tcgacagacg gtgtgcaccg gtcgacatgg tctgagcacc
     1081 ggtggaaacg atctgagcag cggtgcacag ggtctgtgca cgggtccgta gggtcttttg
     1141 tggcgggcgc ggggaattcc gggggggcgg atcttggaaa tgggcgcggc ggagggtgtg
     1201 gccgccgcgg cgcggtctgt ttcctcagcc cggtgtcttc tttggccggc cgcgtttttt
     1261 gggggcggcg tcggcttctt cgcggggggt ttcgggcagg ggggcgagga gggcgcggag
     1321 gtcgtcttcg ccgaatttga cgctgccttc gtggtcctcg gcgaggatgc cggcggcgag
     1381 ttcggccttt ttctcttgca gggcgaggat tttttcttcg atggagccgg ctacgacgag
     1441 cttgtagacg aatacggcct tggtctggcc gaggcggtgg gcgcgatcgg tggcctggtt
     1501 ctcgacggcg gggttccacc aggggtcgta gtggatcacc gtgtcggcgg cggtgaggtt
     1561 gaggccgaca ccgccggctt tgaggctgat gaggaagagg gggacgctgc cttcctgaaa
     1621 ggcgcgcacg ggggcggcgc ggtcgtcggt gtcgccggtg agggtgacga agggaatctt
     1681 gcgcgcggtg agctccgcag cgatgagctg gagcatggcg gtgaattggg agaagaggag
     1741 gatgcggcgg ccttcttcga cgagctcggg gagcatgtcc atgaggaggt cgagcttggc
     1801 gcgttccttg accttggcgg cggcgtcggt cttgaggagg cgggggtcgc agcacacctg
     1861 gcgcagcttg aggagggcgt cgaggatgac gatctggctg cgggcgaagc cgcgggcggc
     1921 gatctcgtcg cggacgcgct ggtccatgct ggcgcgcacg gtttcgtaga ggtcgcgctg
     1981 gcggccgtcg agttcgacgc tgcgcacgac gatggtcttg ggggggagct ctttggcgac
     2041 gtcttccttg cgccggcgca ggatgaaggg ggcgatgcgg cgggcgagga tgtcgcggcg
     2101 gatgaggtcg ccgtgctttt cgatgggggt gcgccagcgg gtggtgaagt ctttggcgtc
     2161 gccgaggaag ccggggagga ggaagtcgaa ctggctccac agttcgccca ggtggttctc
     2221 cagcggggtg ccggtgaggc acaggcggtg gtcggcgcgc aatccgcgca cgacctgggc
     2281 ggcctggctg gcggcgttct tgacggtctg ggcttcgtcg aggatgaggg agtggtaggc
     2341 gtgggcagaa agggcgtcgc ggtcgcgcca cagcagcggg taggtggtga ggcagatgtc
     2401 gtgctcggga atccgcgcga aggcgtcggc gcggctgctg ccgcgcaggc cgaggacgcg
     2461 cagcgcgggg gcgaagcggg cggcttcctg ctgccagttg aagacgaggg aggtggggag
     2521 gacgacgagg gcgggtttgt cgaggcggcc ggcgtgtttt tcggtgagga ggtgggcgag
     2581 ggtctgggcg gtcttgccga ggcccatgtc gtcggcgagg atgccgccga ggcggtgctg
     2641 gcgcaggtgt tgcagccagg cgaggccttc gcgctggtag gggcgcaggt cgtgggcgaa
     2701 gccggcaggg ggcgggacgg gggcgatgcc gccggcgccg cgcaggcgtt cgatccaagc
     2761 gtccacgctg gcgaggcctt cggcgcgcca gccgtcggca aaggcttcgg cgaggcgggg
     2821 ggcgtcgagc cgggagactt tgagcgcgcc ggcggggctg tcgaagaggt cgacgagggt
     2881 gcgggcgatg ggtttgaggc ggccggcggg gagcacgatg cggtcgccgt cttcggtgag
     2941 gatggtgacg ggggcgtcgt cgggaatggc gtccacctgg gcccggtcga gccagcggcg
     3001 gtcttgccgg aagacggcgt ggagcatggg ggcgaggtcg cggcggtggc cgtccacctc
     3061 gacgcccagg gcgacggcga agccgccgtc gtcggtctcg ctgatgtcgg caaaccattc
     3121 gccgggggtg agcacgcggt ggcggaagtc ggcggggcag tcgatgcgcc agccggcgtc
     3181 gcgcaaagcg ggggcgagca gggagaagaa gtgggtccag tgggcttcgc tctcgaggcc
     3241 atggagaccg gccggcgtgc tgccggcgat catcagccag ccgggcttga cgggctggaa
     3301 gccggcgttc tggaaggcgc gccaggcggc ggcttcggcg gttttgtcgc gggcgatgcg
     3361 cacggtgcgg ccgtcgggca gggcggcgat ctcgtgggtg ctgccgggct cgaagcggac
     3421 gtcgccgtag tggaaggcgg ggatcgccac gtcgtagtcc tgccccagga aatccgccgg
     3481 gtagctgcgg tgcttgcgcc agctccagca gcgcagggat tcgagctgca tcacgggctg
     3541 gcagggcacg tcgaggacgg gcagctcggt ggcgtcggcg gcgccgggac ggggcagcgc
     3601 gggcgcgacc tgggccaggg cggcggccac cacggggatt tcggtggtcg acaggggcgg
     3661 cagggcgagg acttcgagga gggcgccgcc gccgtcgatg ttcacgcggc cggcttcgcc
     3721 ggtggccgaa tcgatccagg cgaggggttc ggtgcggatc accaggggcg cggcgggggt
     3781 gacggccacc cgcggacgca ggcccgcttc gtcaggtgcc cagatcagcg cgccgggccg
     3841 cggggcggcc gcctgcaggg gctggatgcg gccgttgtcg tccagttcca gataggcgcg
     3901 gccggtggcg agggcggctt cgaaaagggc caggccttcg gtgccgcgca ggcgcgggcg
     3961 gccgtagccg gcgccgtggc ggacgacttc gcgcaggagg cggaagacct gcaggtcttc
     4021 gtcacggacg aaggacgggg gcttgagcag ggcctggtcg tagttgtacc aggcgctggc
     4081 gcggccgctg ggatgcccgt cggccccgac gcggcccttg agcaggccga actccacgtc
     4141 gtcgccgtat tgcgcggcgc tgcacaggta gaagatggct tcgctggcag cagccttgcg
     4201 gcggttcttg tcggccttgg cgaggcgttc gcccaggctg cgggcccagt tgaggatctc
     4261 ggcgcggggt ttgtccacca gggcgccggc gcggcgcgcg gcgagcacca gggcggcggc
     4321 atgcttgcag cggttgccca cggggcaggt gcagcgggtg gcgagctcga tggagctctt
     4381 gccgaagaat tcgcgccgct cgatgcgtac ggtgacgtgg tagggcgcag cgtcggagcc
     4441 ctgcacgagg gcctggatct ggtggccggc ggcttcgatg tgctgcacgc ggcccacgta
     4501 ggcgcgggcc ttggccaggg cgttggcgga gaacagctcg gcgagcaggg cgtcgtcgat
     4561 ctgggcgaca aggcgttgca ggtccatgac aagaagaagg tggacgcgga attgcggatt
     4621 ctacgtcagc ccacggccct gcctctcagt ccggacggat gccctccagg cccagtccgg
     4681 cggcatcgag gagcgcgggc agcgcctcgc cggcgcgcag gggcaggcgc atcgagacga
     4741 tgtcgctgac cggcgtggtg ccggggttga tctcgacggt ggggatgccc tgctgggcgg
     4801 cccacaccac gggcccggcg atgtagggga aggcgctgcc ggtgcccacc gagaagacca
     4861 ggtcggggcc ttcggcgagg gcggcttcga agcgctgcac ggcggcctcg ggcaggttct
     4921 cgccgaagag caccacgtcg ggccgcagca cgccgccgca gtgggggcat tcgggcggga
     4981 tcgacaggcc ggcatagtcg gccacctcgc gggcacgtcc gcagctcacg cagcgcaggc
     5041 ggtgcagggt gccgtgaatt tcgatgacgt cccgggaacc tgccccccgg tggaagccgt
     5101 ctatgttctg ggtgagcacg gtgacgccgc cgcgctgggc ttccaggcgg gcgatggcgt
     5161 agtggccggc gttgggccgg gcgctgcgac aggcggattc gatctgcgcc agatagcgcc
     5221 aggtgatgtc gggccgggac gcgaacaccg cgccggagag ggctttttcg atgggcaggc
     5281 cgatgtcggt gacggtgcct tcgtaaagtc cgccaacgcc ccgataggtg ggcagaccgg
     5341 agtcggcaga catgccggca cccgtgatga acaggacgcg ccgtgcggtc gataagaggc
     5401 gacccacggc ctcaatggcc gacgagtgat cgtttaaggt atcgaatgtc atgccgatgg
     5461 cttcttgaag aggggaaaag gcggcgagat gcggcagtgc ttacggcatt cccgccacgg
     5521 gccttgaacc ggaccactat acagaaacgc ccgctacagc cgaaatcagc gtcatgtgcc
     5581 aattgcttgg aatgaactgc aacacgccca cggacatctg cttttccttt gcaggtttcc
     5641 aggcccgggg cggcctcacc gacgtgcacg gcgacggatg gggcatcgcg ttcttcgaag
     5701 gccgcggcgt gcgggtcttt ctcgatccgg cggcgagctg ccgcagcccg gtggccgagc
     5761 tggtgcggca gtatccgatc cgttcgctca acgtcatcgc ccacatccgc aaggccaccc
     5821 gcggcatcgt caagctcgag aacacgcacc cgttccagcg cgagctgtgg ggccgctact
     5881 gggtcttcgc ccacaacggc cacctgggcg agttcgatcc gcccctgcgc ggcgatttcc
     5941 gcccggtggg ccagaccgat tcggagcggg ccttctgctt catgctggaa tccctgcgga
     6001 cgcgcttccc cgacggcgag ccggcccgtc cggcgctgtt cgcggcgatc cgcgagctgg
     6061 ccacggccgt cagcgcccac ggggagttca acttcctgct ctccaacggc acctgcctgt
     6121 tcgcccactg cgcgtcgcgc ctgagctaca tcgtgcgtca ggcgcccttt gcgcaggcgc
     6181 acctgaagga ccaggacatc accgtcgact tcagtgaatt gacgaccccc ggcgaccggg
     6241 tggcggtggt ggccaccacg cccctcaccg atgatgaggt gtggaccccg atcgcgccgg
     6301 gcagcctgat gctgtttgta gacggcgcgc cggaagctgc cggtgaggcc tcgacccgcg
     6361 ccttcatccg gaccgaatcc gtcgtggcat gagccgtatc actatttata cgaatcgtgc
     6421 caatgcaaac cgtgtccgcc ctcttatgat gtgggccgat taaaatctct gaatcttgca
     6481 aaaacgagtc aatcacgcca tgccccacag cgcaccgaaa gactgccccc tgtgctttcc
     6541 cgaacgggaa acggtactct ggcaagacgc cagctgtcgg gtcatccggg tggacgatgc
     6601 ggattatccg ggctactgcc gcctgatctg gaacacccac gtggccgaga tgagcgacct
     6661 gtcgacggcg gatcagcgcc actgcttcaa catcctgatg gccgtcgagg tggcgctgcg
     6721 cgagtgctac cggccggaca agatcaacct ggccagtttt ggcaacgtgg tgccgcacct
     6781 gcactggcat ctgatcccgc gctataccga ggaccgccac ttcccccacc cggtctgggg
     6841 tcaagcccag cgccggggcg gggtctcgcc tgccccggcc gtcgacgacg cgcatctggc
     6901 cggcgccctg cgcaacgcca ttgccgagcg cctttccggc tgagcccgga ggatccatcc
     6961 atgtcggaca acgccgtgac cttcaccgaa ctgcgcaacg ccattgccga gcgcctttcc
     7021 ggctgagccc ggaggatcca tccatgtcgg acaacgccgt gaccttcacc gaactgcgca
     7081 gcgtcgagca ataccgcaag ctgctgcgca atctccacac gctcaacgcc tccgagagcc
     7141 acgccaccct ggtgcgcatg ctcgaaggcc tgctcgccag cccgctgggg cccgaggagc
     7201 agctggaggt gctcgaatcc gcccgacaga cgatcacctt cgtccagcac gagatgtcgt
     7261 cccgttacgc agcccatccc ctgccgccgg acagcgccga ggatgcgacc ctgcgccacg
     7321 tggtgcgcct gtggacggcc ctcagcacgg cctacgcccg cctgggcgac agcggcgcca
     7381 acgatgcgga attccgcgcc cagctgccgc tcctcgccca gcgccggatc cagtacctgg
     7441 gccagatcat cgtcgagcac tatcgtgccc accgggccga accgccgggc gcctggcgcg
     7501 ccgtgcatgc ggccttcctc gaagccgagc gggccggcat cacccacagc cgcgtcattg
     7561 acccgctcaa caacacctgg aaggcgcaga gccccaacga ggcctacatc gccctgctgc
     7621 tgatcgacct gaccaacccc tacggccgca accagcgcga gctgacctgg atctgccgct
     7681 gggcccagcg ctttgcgccc tactgcatca tgcagaccac ctgggacaag gacgacacgc
     7741 gcacctacgg cctcgatccc agcgaagacc atggcctgcg ccccatcgcg ctgatcggca
     7801 accggccggg catgcggcgc ttcgacggcc accgcctcgc cgcccagatc aaggccatgc
     7861 tcgccgagtt caagcagggc accacgcccg cctccctcgg tctgggcgac gactgcgccc
     7921 agcccgcctg cgcccgtctg ctggtgtcgc tctatcgccc gtggggcatg tcggcggccg
     7981 gacggcgctt tccgcggcgc ccgacgcgcg gcgtgaatcg catctgcggc gactgggacg
     8041 ccatcggcat gctggtgtcg ggcaaggagt tcgtgccccc caaccggcgc cagatgatgg
     8101 gcagccgcta cgacatgaac atgctcacct ttggcgcccg cgccgacgat gacatcgagg
     8161 acatccgcga tccggaagcc gaggtccagc gccgcggcca cgccatcgac gagtggcaga
     8221 cggtggatca ttcggtgtcc ggattccgcc tgatgcgccg ccagcctgcc cagcgcatcg
     8281 agcaccatca gctggtgggg atcaagcccg aggacggcgg cccctatctg ctgggccagg
     8341 tgagctggct gatgttcgag gcggacggca ccctgatggc cggcatccat ctgctgtccg
     8401 gcctgcccac ggtggtggcg gcgcggccgg tgagcgtcaa tgtatcggcc cacgagccct
     8461 attcccaggc cttcatgctg ccggcgatcc cggcgatgcg ggcccaggcc accatcgtga
     8521 tgcccagcgg ctggttccag gccaaccggg tgctggacat ctcgccgggg gagatccgcc
     8581 agatccggct gaccagcctg atcgcccgcg gtaccaactt cgaccaggcg acctatgagg
     8641 tcatcggcgg ctgaagtcgc tattcggcgg ccgccagccg gcgcgccggg aagcaggcgc
     8701 tgaagcggct gccgcgaccc ggctcgctgt cgatgtgcag atccgcctga tggcggctca
     8761 gcacgtgctt gacgatggcg agccccagac cggtaccgcc ggtctcccgt gatcgaccgc
     8821 gatccacccg atagaaacgc tcggtgaggc gcgggatgtg ctccggcgcg atgccgatgc
     8881 cgtcgtcttc caccgaatac tccaccacgg ccccgcgcaa gcgccaggca atgtggatgt
     8941 ggccgccggc cggcgtatag cgcaccgcat tgaccgccag attggcgaag gcactgtgca
     9001 gttccttgga attgcccaga atgcgctcgg tgccgggcgc cggtgggtcc accacgatgc
     9061 tgtggcgctc ggccgacacg gcgcgggcct cctgctccac atccttgagc atgcgggcga
     9121 tgtccaccgg ttcctcttgg ggcatcggtg cgccggtctc cagggccgac agggcgagca
     9181 gatcctcgat gaggcgcccc atgcgcccgg cctgttcgcc ggccatggcg aggtagtggc
     9241 ggatgtcatc cttgtccagg tcgtcgaggc cgtcgagcac ggtctcgagg aaaccgcgca
     9301 ccacggtgag gggcgtgcgc atctcgtggg agacgttggc gacgaagtcc cggcgcatgg
     9361 tctcgaggcg ctcaccctgg gtgatgtcct cggacatgag caggcgcatg ccttcgccga
     9421 agggcaccac gtgcaccatc aggttgcggc tctggggccg gaactgggag aaggtgaggg
     9481 gctcgtcaaa gcgaccttcg ttcatgtagc gcacgaaatc gggctggcgg accaggttga
     9541 tgaccggcgc acccttgtcg cgggcattgt cgatgccgaa atgcagctcg gcctgacggt
     9601 tggcaaactc gatgaagttg gtgtcagtca ggaacatcat gccgtggggc atggcctgac
     9661 tggcttcgcg aaagcgggcc agcgccgccg tgagcttggc gcgctggtcc tgggcctggc
     9721 ggatcaggcg gttgaggtcg gcgtaaacga agtcccacag gccgacagcc cgcggcaggg
     9781 gcgtgccgac cggctggcgc gcccacttga cgagcttgaa gaggttgccg atgtggtaca
     9841 cgccggccgc cagcaaggcg acgctgaaga ccgtgagacc gggcaccggc ccgaagaaga
     9901 ggcccacgcc gagccccagg acaaagatga cggccaggac gacgagcacc ccgaccacca
     9961 cgtagcggat cggacgaatg acggattcct ttgtgagacg atccgggcca gtcagcccgg
    10021 atgcggatta tcgcactgcc ggctggcttt ccgtctgcgt ggagaagcga tagccgctgc
    10081 cccgcacggt ctgcaccaat ccgtcgtgat gggtcggctc cagcgcgcag cgcaggcggc
    10141 gaatgtgcac atcgacggtc cgctcttcca cgaaaacgtg gtcgccccac acctggtcga
    10201 gcagctgggc acgggaatgg acccgctccg ggtgggtcat caggaaatgc agcagacgga
    10261 actccgtggg ccccaggctg accggcgtgg aaccggcagt cagacgatgg gtggccggat
    10321 ccaggcgcag gccgccgagg tccaccgggt cttcggtgga ttccggtgcg cgacggcgca
    10381 gcacggcctt gatgcgggcc atcagttcac ggggcgagaa cggcttggtg acgtagtcgt
    10441 cggcgcccac ctccaggccc agcaccttgt cctgctcctc gccgcgggcc gtcagcatga
    10501 tcagcggaat cccgcgggtg cgctcgtcgg cgcgcaggcg gcgggcgaaa tcgatgccgg
    10561 acatgccggg cagcatccag tccagcagca ccagatcggg caaggcatcg cgcacgatgc
    10621 gctgggcggt ttcggcatcg cccgcccgca ccacctggtg accggcgcgc agcaggttgg
    10681 cagcgatcag ttcctggatc gcgggttcgt cttcgacaag caggatcgtg gcgggcatgg
    10741 gagcctcccg ggaattgcgt tgtccagagt gtatttcagc atcttgaact tttcgtgaca
    10801 ggtcacgctg ctgaaacgca acgaccgccc cgtgccgaca cgcggcggtc cgggccgttc
    10861 cggtatcatg cgcacagtac gtcattgcac aggaagcttc ccatggccgg cctccagaag
    10921 ggcgatccgc tgcccaccga gatcaagctc caccagcgtt cccgaatcct ggaaatcact
    10981 ttcgacgacg ggcaacgatt cgaattgagc tacgaattcc tgcgtgtcca ttcgccctcc
    11041 gccgaggttc gcggccacgg tccgggcgag gaagtgctgc aactgggcaa gcgcgacgtg
    11101 gagatcgtga atgtggaacc cgtaggcaat tacgccatcc gtccgatctt ttcggacggt
    11161 catgacagcg ggctgtattc atgggactac ctttacgaga tcggcgaacg tcgcgacgaa
    11221 ctgtggcaga cctacctcac ccgcctcgca gtggaggggg gcagccgcga tccggccgag
    11281 ctcccgcctg ccgcgcccaa ggccggcggc tgcggccaca agcactgatc ccggcggcat
    11341 ctgccccggc gcgcccggcg cttccgggcc gatttatcct gtccgaacca tgaacgataa
    11401 aaccacccat ttcggcttcc agaaagtggc cgaagccgaa aaggccaaga aggtcgccgg
    11461 cgtgttctcg tcggtcgcca gcagctacga tgtgatgaac gacctgatgt ccttcggcct
    11521 gcaccgcctg tggaaggcct tcaccgtgca gatcgccggt ctgcgcgccg gtgaccgggt
    11581 gctcgatgtg gccggcggaa ccgcagacct gtccctcgcc ttcgccaaaa aggtcgggcc
    11641 gaccggccag gtgtggctca ccgacatcaa ccacgccatg ctcagccgcg gccgcgaccg
    11701 ggtcgtggat aacggcttcc tgctgcccgt cgcccagtgc gacgcagaga agctgccttt
    11761 tccggacgat tacttcgact gcgtcaccgt ggcctttggc ctgcgcaaca tgacccacaa
    11821 ggaccaggcc atcgccgaga tgcggcgcgt cctcaggccg ggtgggcgcc tgctggtgct
    11881 ggagttctcc aaggtgtgga agcccctggc gccggcctac gatttctatt ccttcaagct
    11941 gctgcccctg atgggcgaga aagtcgccag ggacggcgaa agctaccgct acctggcgga
    12001 aagcatacgc atgcatccgg accaggagtc cctcaaggat ctgatggaac aagccgggct
    12061 cggccgggtc gaatacttca acatgaccgc cggtgtggtt gccctgcacc gcggctacaa
    12121 gctgtaagtc cttcaccccc ggagagaact gcccatgaaa aagctggccg ttgccgccct
    12181 gatggccgtg gcgaccctga ccacggtcgt ccccgacgcc gaagccgccc gcctgggtgg
    12241 cggaaaatcc ttcggcatgc agcgtaatga caccattgtt cgtcgcgaag ccgcgcctgc
    12301 accccaggtg cctccgcgga cggctgcagc gcctgcacag agtgccccgc aggccggcgc
    12361 tccggcaccg caaatgcccc agaaacgttc ctggctggga ccgctggccg gtcttgccgc
    12421 ggggatcggc ctggccgcac tgttgtccca tttcggattg ggcgaacagg cagcgtcctt
    12481 catgctgatc ctcctgggcg tgatggccgt catgatgctg ttcaggctac ttttccgccg
    12541 tggcgcatcc cagcccacgc cccagtatgc gggtatgcct tccacccctg caccagaaac
    12601 ccgtcacgca gcctcggcac ccattgccct gccgacgtcc ggcgcccccg tggccgacac
    12661 cgaggcgctt ccctctggct tcgatgccga tgccttcgtg cgtgaggcca agctcaactt
    12721 catccgcctg caggccgcca acgatgccgg caatctggac gacctccgcg agttcacatc
    12781 gcccgagatg tttgccgaga tccgcctgca gtacgacgaa cgccatggca agccgcagcg
    12841 caccgacatc gtcaatctca atgccgcggt gctggacgtg gccaccgaag gttcgcgcca
    12901 cgtcgtcagc acccgcttct acggcatgct gcgcgaggaa gacgacgctg cgccgacggc
    12961 attcgacgaa gtctggcatc tcgtcaaacc cgtggacggt tcccgcggct ggatggtggc
    13021 cggtatccag cagcgccact gaacgccacg acgaccggta caatggcggg ggctgcggcc
    13081 cccgtttttc ttgcctggag ttgccgatgc tcacatccac cgtcatcgcc gccctcaacc
    13141 atctgctcac ggacgccccc tgggccaggg atcgcctcaa acccttcgcc ggccgcaccg
    13201 ccgtcattca ggtcgcgcct gcgtcactgt gcgtagcagt cgaaggggac ggattcttca
    13261 gcgattccgc caccgccgac acccccgacg ttcgcctcga actgccggtt tcatccctgc
    13321 ccaaggccgt gggaggcatg gacgcgctga tgggcgacat ccgcattacc ggcaatgcgg
    13381 actttgccga cgcgctcggc ttcgtcctgc gtcatctgcg ctgggatggc gaagaagccc
    13441 tggcaaaacg ggtaggcgac attgcagccc atcgtgcgat caatggtgtg cgggatgcca
    13501 cccgctggca tctggatgcg gcgcgcaatc tcaccgagaa tttcgtcgaa tacttcaccg
    13561 aggaacggcc cctcatcctc gcgcgcccgc agttcgatcc ctttgccacc gaaatggccg
    13621 cactgcggga cgatctggcc cgtctggaca aacgcctgag ccgcctcgaa gctgcacgtc
    13681 gctgagcgct ccgttgcgtc accacccgtc gcccagcgca ggtgcgctgc gatcgacttg
    13741 cggattatgc aaggggcact acatggccgt tcgagtccgc tgacctgcgc tcccacctgc
    13801 cagcgcagtg aaagtcgatg ccgccggtga tcgggcatcc acccgtttga tgcagacaaa
    13861 tggccagaac agcggaacag cgcgtcgtca gacttcttgg gcggcacaca aacaaaaagg
    13921 gcagcccttt cggactgccc cttctgtcga aaccgctcaa gcgttcagtg agaacgcttg
    13981 cgcagcttct ggattgccgc gatctgagcc agcgcagatg ccagttcgga ttgggccttg
    14041 gcatattcca gggcagagtc ctggttctgc atggcttctt ccgcacggcg cttcgcttcc
    14101 agggccttgg cttcgtccag atccttgcca cggatcgcgg tatccgcgag aaccgtcacc
    14161 aggttgggct ggacttcaag caggccaccg gccacgaaca ccagctcttc gtcggcctga
    14221 ttctggacct tcactcgcac tgcgcccggc ttgatgcggg tcatcagcgg catgtggccg
    14281 ggcatgatgc ccagctcacc ggcctcgccg ggcagcacca cgaattccgc cagaccggaa
    14341 aagatttgct cttccgcgct gacgatatcg acgtgaatgg tcattgccat agcttgtatc
    14401 ctcctctgtc cggcgggccc gctgggagca ccgccgggcg atggtcgcga tgattagagg
    14461 gtcttcgctt tttcgaagac ttcttcgatc ggaccgacca tgtagaaggc ctgctcgggc
    14521 aggtgatcca cttcaccgct caggatggtc ttgaacccct tgatcgtttc cttcaggggg
    14581 acgtacttgc cgggggaacc ggtaaacact tccgccacga agaacggctg ggacaggaaa
    14641 cgctggatct tacgagcacg ggccacgagg agcttgtctt cgggcgccag ttcgtccatc
    14701 cccagaatcg cgatgatgtc gcgcagttcc ttgtagcgtt gcagggtctg ctgcacgcca
    14761 cgggcggtgt tgtagtgctc ttcgcccacg accagcggat ccagctgacg ggaagtggag
    14821 tccagcggat cgacggccgg gtagataccc agagcggcaa tgtcacggga cagcacgacg
    14881 gtggagtcca agtggaggaa ggtggtcgcg ggagacgggt cggtcaagtc atccgcgggc
    14941 acatacacgg cctggatgga ggtgatcgag cccaccttgg tggaggtgat acgctcctgc
    15001 agacggccca tttcttcggc cagcgtcggc tggtaaccca ccgcggacgg catccggccc
    15061 agcagcgcgg acacttcggt accggccagg gtgtagcggt agatgttatc cacgaagaac
    15121 aggatgtccc gaccttcgtc acggaagcgc tcggccatgg tcaggccggt cagcgccacg
    15181 cgcagacggt tgcccggggg ttcgttcatc tgaccgaaca ccatcgcaac cttgtcgaga
    15241 acgttggagt ccttcatttc gtggtagaag tcgttccctt cacgggtccg ctcacccacg
    15301 ccggcgaaca ccgacaaacc gctgtgctgc ttcgcgatgt tgttgatgag ctccatcatg
    15361 ttcacggtct tgcccacgcc ggcgccaccg aacaggccca ccttgccgcc ctttgcgaac
    15421 gggcagatca ggtcgatcac cttgatgccg gtttcgagca gatccacaga cggggacagc
    15481 tcgtcgaact tcggcgcctt ctggtgaatc acgcggcgct catcgcattc gatcgggccg
    15541 gcttcgtcga tcggacgacc cagcacatcc atgatccggc ccagcgtgcc gtgaccgacc
    15601 ggcaccgcaa tacccttgcc ggtgttcgaa actaccatgc cacgacgcag gccgtccgaa
    15661 gaccccagcg caatggtacg cacaacgcca tcccccagct gttgctgaac ttcgagaacc
    15721 agctcagcac cttccagggt cagggcgtcg taaaccttgg gcatcgcgtc gcgcggaaac
    15781 tgcacatcca ccacggcgcc gatgcattga acgatcgttc cttgactcat cgtcgtttcc
    15841 ccaaacttgt taaaccgtta caccgcggct gcgccgccga caatttcgga cagttccttc
    15901 gtgatcgcgg cctgacgggt cttgttgtag accagttgca gttccgagat cacgttccct
    15961 gcgttgtcgg atgccgcctt catcgccacc atccgggcac tctgttcgga cgccatgttc
    16021 tcggcgacac cctggtacac cagcgcttcc acgtagcgca ccaggagttc gtcgatcacc
    16081 acctgcgcat ccggctcgta gaggtagtcc caggtccctt ccggggttcc gagacgctcg
    16141 ccggtcagcg ggagaagctg ctcaagctgg ggctcctgct tcatcgtgtt gatgaagcgg
    16201 gagtaggcga tgtgcacttc gtccagctca ccattctgga acgcatcgat catgaccttg
    16261 acgggcccga tcagcttttc caggtggggg gtgtccccga gctgggtgac attcgagatg
    16321 accttggctc ccaggcgctg catgaaaccc agccccttgt tgccaatgca ggtcacacgg
    16381 atgtcggtga caccattgcc ttcccaagac ttgagtgcgc tcagcgccac acgcaaggtg
    16441 ttggtgttga ggccgccgca cagacccttg tcggtcgtga ccacgatcag ccccacacgc
    16501 ttggcatcgt ccttcttggt caggaacgga tgcttgtagt cggtgatgtt ggcagcagac
    16561 agattcgcgg cgagacggcg gatcttctcg gcgtagggac gggcagcacg catgcggtcc
    16621 tgagccttac gcatcttgga cgcagcgacc atctccatgg ccttggtgat ctttttagtg
    16681 ttctggacac tcttgatctt gttacggatc tctttgccgc tagccatttc gctctccgta
    16741 tgcccgcgtt acgcccagct cttcttgaac tctgcgatga catcggccag ctgcttgctg
    16801 gcgtcggcat cgagatcctt ggtcgtctcg atcttgttga tcagatcggg cgccttggtc
    16861 ttgacgtact ggtgcatcgc ggcttcgcag gcgagggcgc gcttgacttc gacatcgtcg
    16921 aaagcaccct tgtcgaccgc gaacagcgtg atcgccattt cggaaacctt gagcggcgag
    16981 tactgcggct gcttcatcag ttccgtcacg agacgaccgc gctcgagctg cttgcgggtg
    17041 gcttcgtcca gatcggaggc gaactgcgcg aacgcagcca gctcacgata ctgggccagg
    17101 gccagacgca cgccgccgcc gagcttcttg atgaccttgg tctgggcagc gccgcccaca
    17161 cgggacaccg agataccggc gttgatggcg ggacggatac cggcgttgaa gaggtcggtt
    17221 tccaggaaga tctggccgtc ggtaatcgag atcacgttgg tcggaacgaa cgcggacacg
    17281 tcgccggcct gggtctcgat cacgggcaga gcggtcagag aaccggtctt acccttgact
    17341 tcaccattgg tgtacttctc gacgtactca gcagacacgc gggcagcgcg ctcgagcaga
    17401 cgggagtgga ggtagaacac gtcgcccgga taggcttcac ggcccggcgg acggcgcagc
    17461 agcagggaca cctgacggta ggcccaggct tgcttggtca gatcgtcgta gatgatcagg
    17521 gcatcttggc cacggtcgcg gaagtattcg cccatggtgc agccggagta cggcgcgatg
    17581 tactgcatcg cagcggactc ggaggcggtg gcggcgacga cgatggtgta ttccatcgcg
    17641 ccgttttctt ccagcttgcg cacgacgttg gcgacggtcg aggccttctg gccgatggcc
    17701 acgtacacgc agtacatgtc ctggcccttc tggttgatga tcgcgtcaac cgcgacggcg
    17761 gtcttgccgg tctggcggtc gccgatgatc agctcgcgct ggccacggcc gacgggcacc
    17821 atggagtcca ccgacttcag accggtctgc accggctggg acacggactt ccgccagatc
    17881 acgcccgggg cgaccttttc gatcggctcg gacagcttgg cgttgatcgg gcccttgccg
    17941 tcaatcggtt gacccaggga gttgaccacg cggccgatca gctcgggacc gacgggcact
    18001 tccaggatac ggccggtaca cttgacggtg tcaccttcgg agatgccttc gtattcaccc
    18061 aggaccacgg cgccgacgga gtcgcgttcg aggttcatgg cgagaccgaa gacgttgccg
    18121 gggaattcca gcatttcgcc ctgcatggca tcggagaggc cgtggatacg gcagataccg
    18181 tcggtcactg ataccacggt accctcggtg cgggcattcg cggacagctg caggttctga
    18241 atccgactct tgatcaggtc actgatttca gaggggttga gttgcatagc ttatgctcct
    18301 agttctttag cgcggcggcc atgttcgcga gcttgccgcg gaccgaggcg tcgatgactt
    18361 catccccgac agcgatcttg accccaccga tcagttcggg gtcaacgctg acgctcacgt
    18421 tgagacgggc cttgaagcgg gcttcaaggt cggccacaag ggccttcaac gtggcttcgt
    18481 ccatcgggaa agcggagacg atgttcgcct ccttgacacc ttcgtgttcg ttcttgagag
    18541 caacgaacag gtcacggatc tcgggaagca cctgcagacg ctcgttatcg accaggacac
    18601 ggacatagtt ctgctgttca gcagtcagtc cgcttccggc gacgtcgaga aacagcccag
    18661 ccagttgttg aggctgcagc ttgggatcac tgatgcaatc ccgcatttgc ggatcggccg
    18721 cgatacccgc gagccgatca agtgcttccg accagggccc cagcgcattg cccgcggaag
    18781 cgagctgaaa agccgcttca gcataggggc gtgcgatggt gacgttctcg gccatggctt
    18841 attgaagttc ctgtttcagg ttggccagca gttcggcgtg ggcctgagcg ttgatctcct
    18901 tgcggaggat cttctcggcg ccggcgacgg caagatgggc gacctgatcg cgcagggctt
    18961 ccttggcacg ctgggcggcg acgccggctt cggcttcggc tgcctcacgg gcctgggcga
    19021 tgatgcgggc agcttcggcg cgcgcttcgt cgaggaagga ggcggcttgc ttttcggcag
    19081 cggcacgcac gtccgtagcg gactcgcggg ccttgcgcag ctcttccacg accttcttct
    19141 cggcatgtgc gagatcgctc ttcgccttgt ccgcagccgc cagcccgtcg gcgatcttct
    19201 tcgcgcgctc gtcgagtgcc ttcacgatgg ggggccacac gaatttcatc gtgaaccacg
    19261 ccagaatgaa gaacacaacg agctgggcta ccagggttgc gttcagattc acggtagtct
    19321 tcctttagtc aggttgaacc gcgaacttag atcttgaacg ggttggcgaa cgcaaacatc
    19381 atggcgatac cgacaccgat caggaacgcg gcgtcgatca gaccggccag caggaacatc
    19441 ttggtctgca gggcgttcat cagttcgggc tgacgagcag aggcttcgag gtacttgcta
    19501 cccatgatgc cgataccgat acaggcaccg atagcaccca gaccaatgat cagaccggcg
    19561 gccagagcaa caaaaccaag aacgtgttcc atgacgactc ctttagttga gatgatttaa
    19621 attgaaaccg aaacgactgc taaaactttg aaactcagtg accttcgtgg gcctgaccga
    19681 tatacaccag ggtcagcatc atgaagatga aggcctgcag agcgacgatc aggatgtgga
    19741 agatcgccca gatcgaaccc gcgaccacgt ggccgaggaa accgaacacc gtagcggtac
    19801 cacccatcag ggcgatcagc atgaacacca actcgcctgc gtacatgttg ccgaacagcc
    19861 gcatgccgtg ggatacggtc ttggccagga actcgatcat ctgcatcagg aagttgatcg
    19921 ggtacaggag cggatgagaa ccgaagggcg cggtgaagag ttcgtgcacc cagccgccaa
    19981 aacccttgat cttgatgttg tagtacaggc agatcagcag cacgccgata gacatgccca
    20041 aggtgccgtt gaggtcggcg gtgggcacca cgcgcaggaa ggcgtgctcg tcgccacaca
    20101 tgatcgccca gatcttgggc agcagatcca ccggcaggaa gtccatggag ttcatcagga
    20161 aaatccagaa gaacaccgtt agggcgacgg gggcgacgaa cttgcgggac tcggcgctat
    20221 gcacgatgcc cttggcctga tcggcaacca tctcgacgag gatttcgatg gcggcctgaa
    20281 agcgccccgg cacgccggag gtggctttgc tggcagcctt gtagaggatc agcagggtga
    20341 cgatgcccag gccgacggag aagaacaggg tatccagatt ccagacactc cagtcgatga
    20401 cggcagactg ggcgtggccg gtggagttga agtgggtcaa gtggtggacg atgtattcgc
    20461 ccgcggtcgg tgcgtgtgct tcgtggccag tgctcatggt caggtcttca ccaaaaatgc
    20521 aaataaattc gctttaagcg ccacgaccaa cccgccaaac aacgccaacc aatggacgtc
    20581 tggatacacg agtcgaacgg tcgccaacaa tcctacggtt gacgcgatct tgaagaactc
    20641 gccaacgaaa aacgcaacag ggtacgacgc gtccggccgc ttggtaacca agtgcaggcg
    20701 caaagcgaac agcaaattgg gaagaaaaca ggctgcgcct cccaacgccg ccgacaacgc
    20761 accgcgcaat cccaaaaatg ccccggcaat cagtgctgcc aggatcgtcg cgcctatctg
    20821 cagggtcacc gctttgaaca tggatctctg cgaggcacgt cgggtgttgc gccgtccgcc
    20881 gtacgaaaaa cgtgcggatg gtaggggtaa accgcagcaa agtcaaatgt tggtgcagtg
    20941 caccatcaac aaaaaggctg tgtttacgcg ggcgtcaacg gaagtcggcc ttgacaaacc
    21001 ccgcaaacgt atcgaaagtt atcgacatga acaaggacta ctcccgtgcc attccggcag
    21061 cctggcgcga cgtttcggcc gtgtttgccg cgctcggaga tgaacaccgg caacgcatcc
    21121 tgctgacctt cgaggccggc gagcggctta acgtgggcca gattgcggaa gtctcgaccc
    21181 tggcccgttc caccgtgtcc caccatctca aggtgttgcg cgaagccggt gtcctgtctg
    21241 cccaacgtca gggcaaggag atctatttca gtgtgaatac gcactttctg cagaaggcct
    21301 ttgcgcgggt ctctgactac ctggagactc gactgtgagc aatgtccggc gcgttccatg
    21361 ggatcaggac gtggccgggc ccggggcacg tcacggcatc ggaaccgcgg cgaagtgcgc
    21421 ctccgatcgg tcgcggctgg cgacagtctc cgtgccaccg tttgtcttcc gcctacggcc
    21481 cagaccgtcg gatcgcgtgg atcctgccca acccacaccg ctacgccgac gggtttccgg
    21541 caaggatcga tcacccgcgc acgcgccatc tgcgaccgga ttcatccgtt cgacggcatt
    21601 cgcacggcgt cccgtaccgt cctacgatct cgatgcaccc caaaacggct caagcttggg
    21661 cgcagcccag atgcgcccga cagccgcctg cgtaccgaca ctggagtgat ccatgcgacc
    21721 cttcccgtcc gcccttgtgc tttccctcgc cgcgtccgtc agcattgccg gatgcgcgac
    21781 gtggttcaat cccgcacccc atactcaacg ggccacggtg gtcgagtacc tctatcccaa
    21841 gggcgaacag ccgaccctca cgccgtcgat acccgaacta catctgccct tgcgcgtcgg
    21901 gatcgcgttc gtgcccagcg aacgtttcga cgacctcccc gagaccgagc gggaccgcct
    21961 gttaaaccgg gtcagggacg cttttgccaa ccgcccctac atcgctgcca tcgaaatcat
    22021 tcccacggcc tacctgcgcc cccgcggcgg cttcgacaac ctggagcagg cggcgcggat
    22081 gttcaacgtg gacgtcgtga ccctgctgtc ctatgaccag gtccagttca gcgacagcaa
    22141 tcgtctctcc gtcttttact ggaccctcat tggcggctac atcttcaacg gcagccagta
    22201 cgacgtgagc accctggtgg atgcttcggt cttcgacgtg aagtcccgca gcctgctgtt
    22261 ccgtgccccg ggcaccagtc agatcacggg cagttcgccg ctggtgaact ttgccgaagc
    22321 ctcccgcgag gcccgcagcg aagcctaccg gcgcgcgatc gatgtgctga tcccccggct
    22381 cgatgcgcag ctggaagcct tcaaggtccg cgtcaaggac aggcaggtcg cccgcatcgt
    22441 cgagaagccc ggatactccg gcggtggagc cctcgacccc gtcgccctcg gcctccttgg
    22501 aacgggcgca ctgctggccc tcgcctggcg gcgtcggtca gcctgagccg gtgcgggtcg
    22561 aacgcctctg tgccggcgcg agcgtcgcat tcctcatgct cgtcaccctg ctccccgccg
    22621 acgcggtggc gtggcgtcgc gatgcccttg gcgacggtgc ctggtggcgc ctgtggaccg
    22681 gcccgttcag ccattggtcc gcgctgcatc tggccggcaa cctgactgcc gccgctgcgt
    22741 tgagcgtcct gatcggcccg ccggtctggc gctggatcgc cgtcctgccg gcggtcgcac
    22801 cgttgctgtc cctgttcctg atcgccagcg cgccggacct acagcattat cgcgggctct
    22861 cgggcctgct gggcgtgctg gtggtaggtg caggggtcga aggcggggtg atcggccggc
    22921 tggtggcggg tctgtacgtc gtcaagctcg ccgtcgacgc catcgagggc gcttcctcac
    22981 cggtgctgcc gacgggggtg gccgtcgtct ggcaggccca tctgggcggg ctgctgctcg
    23041 gtctcctggt tgcggcctca ttccacctgc accgacgcgc cagcgcgtcc acgccgacac
    23101 ggaccccttc cgatacgcca tgagtaccca gttccatctg ctccgcgagc ggcgctttct
    23161 gcccttcttc gtcacccagt tcctaggcgc cttcaacgac aacctgttca agaacgccct
    23221 ggtcgtggtg ctgaccttcc aggccacgcg atggacgacg atccggccgg agctcctggc
    23281 caatctggcg gcaggcctct tcatcctgcc ctatttcctc ttctcggcca gcgcaggaca
    23341 gctggccgac aagtacgaca aggcgcgcct gacccgcctc gtcaagctgc tggaaatgct
    23401 gatcatgggc gtggccctgg cgggattcct gctccacacg ctgatggtgc tgttcgtcgc
    23461 cctgttcctg ctgggctgtc attccaccct gttcgggccc gtcaagtacg ccctgctgcc
    23521 ccagcacctg cgggaagacg aactggtggg cggcaacgcc ctcgtcgaag ccggcacttt
    23581 cgtggcgatc ctgctgggga cgctcgccgg cgggctgctg gcgggcatgg actccgcgga
    23641 gcgctggatc gccgtggcgg gatttctggt ggctgccaca ggctacctga gcagccgcgc
    23701 gatcccggcc gcaccgccgc ctgcgccgac gctgcgcatc catcccaatc ccttcaccga
    23761 aacctggcgc aacatcggct tcgcgcggca gaaccgcccg gtattcctgg cgatccttgg
    23821 aatttcgtgg ttctggctct acggcgccct gttcctcgcc cagttcccgg cctttgccaa
    23881 gaatgtgctg ggcggcggcg aggggaccgt cacgctgctg ctggcggtct tcacggtggg
    23941 catcgggctc ggctcgctgc tctgcgagcg cctctccggc ggccacctgg agatcggcct
    24001 ggtgcccttc ggctccatcg ggctcaccct gttcgggctc gacctggcct tagcagcgcc
    24061 cgcaggcgtc ccggccgctg cgccactggc gctttcgggg gtgcttgccc agcctagcac
    24121 cctgcgtctc ctggtcgccc tgctgatgct gggcatcttc ggcggcttct tcatcgtacc
    24181 cctctacgcc atgatgcaga gccgctcggc agtgggacac cgggcgcggg tgatcgccgc
    24241 caacaacatt ctcaacgccc tgttcatggt ggtcggcgcg ctgggagccg cggcgctgct
    24301 tgccgccggg ttgtcgatcc cggccctgtt cgcagccgcc gcggtgatga acgccgccgt
    24361 ggcgctcttc atctaccggc tggtgccgga attcctgttg cgcttcatca tctggctgct
    24421 ggtccacagc gtataccgct tgcgcacgga agggctgccg cacctcccgt ccgaaggtcc
    24481 ggcggtgctg gtgtgcaacc acgtgagctt cgtcgatgca ctggtgatcg ccgccgcctg
    24541 tcgccggccg atccgcttcg tgatggatca ccgcatcttc cgctggccgg tcctgtcctt
    24601 cgtgttccgc catagccgcg cgatccccat cgcaccggcc agggaagata ccgcgatgat
    24661 ggaggcggcc tttgatcagg tggccgccgc gctggccgaa ggcgaactgg tggcaatctt
    24721 tccggagggg cagatcaccc gcgacggcga actcaacgcc ttccggccgg gcatccggcg
    24781 catcctcgcc gccagccctg ttcccgtcat cccgatggcc ctgcgcggac tgtgggggag
    24841 catgttctcc cgcaaagacg gcccggccct cagccgaccg ctgcgacgcg gcctcttcaa
    24901 tgccatcgaa ctggccgtcg gtgccccact gtcccccgag tccgccacgg tggagggtct
    24961 gcgcgcgcgg gtccttgccc tgcgcggcga acgtcgttga acggctccta cttcacgtgc
    25021 cccacgcacc atgtcctacc tcatcaccct gatccaggcc ttcgtcctgc tgatcctgct
    25081 ctgcctgatc ctcgccggag gcgtctgcgt tgcctcattt cccaccgaac ctgcggcggt
    25141 ggtggtcggg ctgatcagca tgctgacgtt cagctggccc gcatggttgc tgatccgcac
    25201 gttccggtcg cgaccggcgc ccaaaacgga acccgagcgg tccgaccctc ccacaccgtg
    25261 atcgccgatc ccgccaccgc gcacgcactg cacgccgtgt tcgaggccct ggccttgttc
    25321 ggtggagccc gcctgtacgt ccatcagagc cagcggcagt cgcggcacag tgtctttcag
    25381 ggaggccgat ttgccgtact gtgcggctgc ctgctcggcg cagccatcgg caacaaggtg
    25441 gtgttctggc tcgaaatgcc ccagcttttc cagcaggccg acggctggca acggatcttc
    25501 gctggtcagt ccgtggtagg cggcttgctg ggcgggctga tcggcaccga aatcgccaag
    25561 gcactcagcg gcgagacggc gtccaccggc gacggcttcg tggtgccggt cctcgccggc
    25621 atcgccgtcg ggcgtgtcgg ctgctttctg gcaggtctgc acgatggcac cttcgggcgt
    25681 ccgaccgccc tgccctgggg gatcgatttc ggtgatggca tccctcgcca tcccacccag
    25741 ctctacgaaa tcctcttcgt cgtgaccctg gccggcctgc tgcagcagct gtcaccccgg
    25801 ctcggtgcgc agcccggtct gcgcttcaag ctgatgctaa gcgcttacct gctgtggcgc
    25861 ctcctgatcg atgggctcaa gccgctcccc tatgcctggc ccttcggact ctcgggcatc
    25921 caatgggtct gcacgcttgc cctgtccatc taccttcccc tcacgatccg ccaatggcgg
    25981 cagcgccatg tcacgcaaga accgccccta cctgttctat gacacgaccg cttcggtgtg
    26041 cagcacctgc ctgcaccggg tcgaggccaa agtcctcatc aaggatgacc gggtctacct
    26101 ggaaaagtgg tgcccggccc atggccggga acgtgtcctg atcgcggatg acgccgacta
    26161 ctaccgcctc gcccgcgagg tctatgtgaa gcggccggaa atgcccgagc acttctccac
    26221 gaccatgacc tggggctgcc cctacgactg cggtctctgc cccgaccaca tgcagcactc
    26281 ctgcctcacg gtgctcgaga tcacggagca ctgcaatctg aagtgcccgg tgtgttacgc
    26341 cgattccggg cccgaacgcc agcttcaccg cagcctgggc gagatcgaag cgatgctgga
    26401 cgccgtcgtg cgcaacgagg gcgaaccgga cgtggtgcag ctttctggcg gcgaacccac
    26461 gctgcacccg gatttctttg cgatccttga cgccgcccgg gcgcggccga tccgtcacct
    26521 gatgatcaac accaacggcc tgcgcatcgc ccgcgatccg gaattcgttg cccgtctggc
    26581 cgaatacgga cgcgggctgg agatctacct gcagttcgat tccctgcggg ccgaagcgct
    26641 tcacaccctg cgcggcgccg atctgacccg catgcatgcc caggcgctcg aagccctgga
    26701 acaccacggc ctgtcgacga cactggtgat gacgctgcgg aacggggtga atgaccagga
    26761 gatcggcgac gtgatccgcc acgggctggg ctatcgctgc gtgcggggcg tgaccctgca
    26821 gcccgttcag gatgccggcc gcaacctggg cggacggggg gccgaagacc ggctgacggt
    26881 cagcgggatc cgccgacgca tcgccgaaca gtccggacta ttcaccctcg acgacatcgt
    26941 cccggtcccg tgcaatccgg acacgctggc catgggctac gccctccggc tggacggtca
    27001 ggtgcatccg ctcacccgct ggctggggcc cgaggccctc ctcgccgggc ccgccaacac
    27061 catcgtcttc gagcgcgatc cgtcgatccg ggatcgcctg ttccggctct tttccaccaa
    27121 tcattcaccc gcgtccggtg cccgctgcct gtcggacctg ttgtgctgcc tgccccggat
    27181 cgacgcaccg acgctcgatt acacgaatgt cttccggatc ctcatcgtgc agttcatgga
    27241 cgccaccaac ctggacctgc gggccctgaa gaaatcctgc gtacacatgg tgcagccgga
    27301 cggccggatg attccgttcg aagccttcaa cctcttctac cggggccgcc tgaccgaagg
    27361 gctggagacc ctccgcgccg gcgttgccga cgacatcgcc aggcgccacc gatcggtgcc
    27421 ctcagcgggc taacgccgac cccgtccgtt caaggctcag caccagccgg cctgcgtcgt
    27481 cgaggagcag caaggtggcc ccttcgatac gccagtgtgt gacctgatcc agcaaagcgt
    27541 ggaagccgac ctctgcgtcc ataccctgac cacaggccat cttcgtgccg gcgtgcgcac
    27601 tgacgcgcag gtggtcgccg tccacccggt aggtcccgat gaaccggttg caccctccgg
    27661 ttccagccag acgtcccgcc tccagaaagt gcagctgagg tatccggacg ctgcgctgcg
    27721 gatccaccgg aaaaccatcc tgagtgatca ggcgccaggg cgctccgcgc agaccaccgg
    27781 aatcgcctgc gaccggcggg cgtgagctca cgcgacgcag caacagcgtc accgccccat
    27841 cgggaccccc atcaagcacc ggatagacct ggtccgtgac gaaccacggc gcaccaccga
    27901 cactgatgcg gccccgcagg ctgtagcgcc tgtgggcctc gacatctgcc ggatccacct
    27961 cgaccgcaaa gggaatgggc ggcgcaccgg cgggctcgag gcgcgtccgg ccgatgaccg
    28021 gtgccggcgc atcggctcgg gacacgtctt ccaccgtcac ctcgagcacg gcctccggag
    28081 gaagccgcag acgctcccgg tagatggcag atccgtggat gagctcggca cccacgggta
    28141 ccacacacag gagcaaggcc gcagccatca gcaggcgagc acgcatcagg gcgctctcag
    28201 gcaggactta tggacggggc ccatggacgc agcgcgccga tgcggtcccc cgcatccatc
    28261 ggtcagccca ggttcttgag ggcgaattcc caattgatga gcttgtcgag caccgcattg
    28321 acgtagtcgc cgcggcgatt ctggtaatcc aggtagtacg cgtgctccca gacgtccagg
    28381 gtcagtaggg gcgtgtagcc ctgggcctgg ggcatgtcgg cgttgccggt cttgaccacc
    28441 ttgagcgcct tgccgtccgc caccagccag gcccagccgc tgccgaactg ggtcagggcc
    28501 gcctgggcaa gttccttttt cagtgcgtcc acactgccga aggacgcttc gatccgggct
    28561 ttcagggctg cgggcggctc gcccccccca ttcgggcgca ggctgtacca gtagaaggtg
    28621 tgattccagg cctgcgccgc attgttgaag atggcggtct tgtcggcctt gcccatgacc
    28681 ttggcgatga tctgctcgag gctgaggtcc gccagttcgc tgccggcgac cagcttgttc
    28741 agattgtcca catacgcctt gtggtgcttc ccgtagtgga agcccagggt gttggccgag
    28801 atcaccggcg ccagggcgct gtcggcgtag ggcaaggcgg gcaggacaat gggactggca
    28861 gcctgggcgg ggcgcacgaa gccgccgagt gccaacgcgg acacggcccc cagggaggca
    28921 ccgaggaact gacgccggtt gaagccggac ggggaatcga aggtggaacg ggaatccatg
    28981 gggctctcct tgcaggtggg gaatgcggat ccggtcgggc gaatccggct tccatcatta
    29041 ttggccaagg cggacgccct tgtcatggtt cccttgcagg gtctcaggtt ggtgcgtgaa
    29101 gggtgcgcaa gctgaatccg agggtggtgt cgccggacga aaaccagatc tggccgtcct
    29161 gcagggtaca ggtgagcgtc atggaacgtt ccgcgagggc cgccagggcg cgggaatcat
    29221 ccgtctcgag ggcctggacc gtcagattgc caaggcgcgc gagggccgcg ctgtcctttt
    29281 gccaccagat gtccacggac cggcccccat aggccagcac gcggacctgt tcggcccgcc
    29341 cgctggcccg gcgcagcagc ttctcatccg gcagtccaac ctcgatccat tccaggatgc
    29401 gcccggtgtc gtccttgagc cacgcggccg gctcatcctc ggaggacagg ccgcggccga
    29461 actgcaggcg ctcatcggcg tacaacgcaa aggccaggac gcgcaccatc atgcgttcgt
    29521 ccgtttcgga cggatgccgc gccagggtca ggacgtgctc gccgaaatga ccgcggtcca
    29581 ggtcggagac cgtcagcgag gcacggaaaa tggtggattt gagcgccatg ggatcaggcg
    29641 ggtggtcccg ctgcaatgaa aaaccgggcc ctacggcccg gttctgttcc ggtgacggaa
    29701 cggattactt gccgaccgcg gccttggcct tgtcggcggc ttccttggtg gcatcggcag
    29761 tggccgcaac agcttccttg ccagcttcct tcgccgcgcc agcagcctcc ttggcagcgg
    29821 cgaccgcatc cttggccgcg tccttggcgg caccggcggc ggccgtacca gcttccttgg
    29881 ccgcaccggc agcatccttc gcagcgtcct tggtggcttc ggcggcctgc ttggcggcct
    29941 cttcagcctt ctgggcggct tccttggctg catccacgac aggcgcgggg gtcggcgcag
    30001 gggcctcttc cttcttggag caggcggcga ggccaagagc gatcagggcg gcaagggcaa
    30061 gggcacgagt ggtcatctgg ggttctccaa atcagcgatt tgtatcaaaa agtcattgaa
    30121 aaccataggt tttcagtgtg tgcgagaggc taacgcggca aagtgccgaa aagatgacgg
    30181 atcgacaaaa aaaggccccg aagggccttg cacgcggcgc aacgatcgcg cggatcactt
    30241 cacgcggttg cggtactcgt cggtgcgggt gtcgatttcg atcttgtcgc cgatttccac
    30301 gaaggccggc acggacagtt caaaaccgct gggcgcgatg cgggcgggct tcaggacctt
    30361 gccggaggtg tcgcccttga cggcgggttc ggtgtattcg acttcacgga ccacagaggt
    30421 gggcagatcc acggagatgg ccttgccgtt gtagaacacc acttcgcagg gcatgccgtc
    30481 caccaggtac ttgagggcgt cggtcatgtt ctcggcttcg acctcgaact gctcgtagtc
    30541 ggcgtccatg aacacgtaca tcggatcggc gaagtaggag taggtgactt ccttgcgctc
    30601 gagaaccacg acttcgaact tgtcgtcagc cttgtagacg ttttcggacg gggcgccggt
    30661 caggaggttt ttgagcttca tcttgaccac ggcagcgttg cggccggact tgttgtattc
    30721 ggccttctgc accacgaggg ggtcgttgcc gaccatgatc acgttgccgg agcggagttc
    30781 ctgtgcggtt ttcatgctgt ttcctgcttg cgaagattgc gcggcacgtg ccacggtaaa
    30841 taggtaactt actattttag ctgatctttg ctgaattgca ccagaccagc ggcgagttca
    30901 ccggcctgtt gcaggtgggc gctccacgcc ggtgcagccg cggacagcgc gggcagggca
    30961 tccgcaaagg cctgccaggc ccgggccgcg tcgtgtccgc ggttccacgc ccgccagaaa
    31021 tcccgcacgg cagcgccggc cgacggcggg aggcgctccg cgtagcgggc caggaaggca
    31081 tcgagcttgt ccaaatgggc atcgtcgtcc tggcggtaga tctgccacac gaaggggcgc
    31141 tcggcccact gggcgcgtac gaaggaatct tccccccgca cgaagttcag gtcgcaggcc
    31201 cacagcaact cgtcgtagcg ggactgctcc acgaatggaa tcacgacgac ccgcagggca
    31261 ccggcctcga tccggtcgcc aggcttcagt ccattccgtc ccaggcactg cgcgacctgc
    31321 ggcagcaccc gcccttcagg caccaggcag gtgagcggcc tggtaccgtt ccgccagacc
    31381 tccagcagct cgggcagggc cgcattctcg taggcaaaca aggagatccg cagctcgccg
    31441 ggccgcgcgg ccggcagacc cagcccggtc aggcactgca cgggtgcgtg ggcggcccga
    31501 cggacgtcgt agtcccgctc ccgcaacagg ccgccgctgc tggcatcgaa gccgggaaag
    31561 tagaagtact gggtcagggg cagacggggg tggggggaag gcaggcgatg gcatccggcc
    31621 acccagtctt cggcgctcag gtattcgaga ttgatccaca gggggcgggg caggcgcagc
    31681 gccatcgctt cgagaaagcc cgccggcagg ctgcaggcaa aggcgccaat gaccacatcg
    31741 ccaggcacga tggccggcaa aggatcggtc cacgcgcgga tctcgacacc cgcccgatgc
    31801 tggatcgcca gcaccggttc gagggacggg atcagacgtc gcgcactggg cagatcgtcc
    31861 acccacaagc gcggctggac gccatgggcc accgccagtt cccgcgccag gcgccagcac
    31921 accccgatgt ccccgaagtt atccacaact gggcagaaga tgtcccagca gggacgggca
    31981 tcagatgagg aacccggggt ggaattgttc aaggcgggga taaacaggca aataggacgc
    32041 ggacagattg tgcacaaacc ggcaatccga ggcgagcccg cctttcctca acattctgcc
    32101 cccacgccgt cctgcccgct gccgacacgt ttatcaacat gacaatcctt tgtttccatg
    32161 gggattattc atgttgtcca ctgtttcgtt ccgagcgtta acagaagtat tgttttaata
    32221 agaaaagtct acacacttct gatcccatgc gcccccggca gaacgtcaca tggccggctt
    32281 acacttgaaa cctcacccag gagaatccca tgctctcaat caccgacctg ctcgatttca
    32341 ttgatctgga ccgggatacg gtactgatcg tggctgaagc gaccggtctt ccccccgagc
    32401 cggccatcat ccttgcgcgg gaactgctgg ccagccgacg gggcatcctc accttgcatc
    32461 acatgttccg ggacgagcgg gcccgcgcag atgaacgtgg tcatgtcctg cgggttcggg
    32521 atctggatcg cgcttaccgg cactttgccc gaaagtaccc gcagcccgtc gtggtgtgaa
    32581 caaacagggg agattcccgc gcaggaaatg tggacaagtc ctcgatggaa tgggaggccc
    32641 gatttcctcc ccaatgcctc cccgtggacc ccacatggct ttccagagct ttttcaagga
    32701 tccagtacat tgatcttgct gaggattctt gggttatcca cagaatcttc cggtacacca
    32761 ttattattat cttgtttaca aacaagaata ttgaacaacc aagattgccg cgaaatgcgc
    32821 cgtgtcttgc ctgtcaggcg tgatccagga cgtcgcccgg cggacggagc tgccataatc
    32881 cggtcaccct cctgtctggc ccgtcacgcg tgtctgcccc gtcctcgccc atatcccgcg
    32941 ctgtccgcca cccctacctc ttgctgacgc tgacggtcct gttctggtcc ggcaacatgg
    33001 tggtcggtcg tgccttgcgg gaggccatac cacccatgag tctggccctc gggcgctgga
    33061 tcctggcctt tttgctgacc ctgcctttcg cgtggcccca tcggaaccgc ctgcagacgc
    33121 tcgaccgccg tgagtggggc atcctggcgg tcctcggggt gctgggcgtc ggggcttaca
    33181 acaccctggc ctacattgcc ctgtcccaca cgacggcgac caatgccgcg ctgctcaact
    33241 ccttcatccc ggtggccatt ctggtcctca cctggtggct gggacatcgc agccggccgc
    33301 tggagatcat cggagtcggg gtttccctgg caggtgtgat gaccatcctc agtcacggcg
    33361 agctccgggt gctcacccat ctgcagctca acatcggcga tgtatggatg cttggcgcgg
    33421 tgctgtcctg ggcgctatac acggtgtgcc tgcgctggcg tccggaaggg ctccatccca
    33481 tggccctgct ggcggtgctg accgccatcg ggctgatggt gctgacgccc ctggtagcgt
    33541 gggaactggc cggtggtgcg cagatccggc ccgacgccgg acttctcgcg gggatcgcct
    33601 acaccgggat ctttccggcc tttctcggct acgtgttcta caaccgggcc gtcgcccagg
    33661 taggcgctgc caccggcggg ctgttcatcc acctgatgcc ggccttcagc accctgctgg
    33721 cgatggtctt cctgggcgag cagccgcatc tgcatcattt tttcggcatt gcgttaattc
    33781 tggcggggat cgggctgacg acgggtttca agaaccgcta aactcggcgg cctcatctgc
    33841 ggagctgcgc cgtgcgtttc gagcacctcg tcgagatcaa cgatcccctc aaccccctca
    33901 ttccgtccct gacgcgggaa caggtctggt ccggcctgat gctgcgggtg gatgagccga
    33961 cggtcttcct tcccggtctc gaacgctgcg aaatcctgtc gcgggcggcc gaccaggtcg
    34021 agcgccggct ccatttcggc ggcgtgcagg tgcgggacac cgtgaccttc agcccgctgg
    34081 agtgggtgcg cttcgaatcg tccgccaacg cggagcacgc gggcgggcgc ctgacgatcc
    34141 gcctcgaaca gcccgatccc ctgcgtcttt acctgcgctt cacctacgag accacgctgc
    34201 cggacggtgg cgcggccgat ggcgtgcagg tatcggagtt cgtcaaatcc gcctatcgcg
    34261 aatcggatgt ggataccgtg cgcatcatcc gcgtgctgtc cgccgggggg cggccccact
    34321 gacgttgcag gatgcttcca gaacacacca gaacagaacc aggagacacc atgatcgccc
    34381 gacccgaact gcgttccttc cagccgcccg tgcgcaccct gatgggcccc ggcccgtccg
    34441 atgtgccgcc gcgggtcctc gccgccttgg cgcggcccac cgtcggtcat ctggacccgg
    34501 cgttcgtggg catgatggac gagctcaagg aactgctgcg ctacaccttt cagacccgca
    34561 atgagctgac cttcccggtc tcggcgccag gctcggtggg tatggagacc tgtgtggtga
    34621 atctggtcag tcccggcgat acggtggtgg tgtgcatcaa cggggtcttt ggccagcgcc
    34681 tcgccgagaa cgtgcggcgc gccggtggcg tgccggtgat cgttgaggac ccgtggggcg
    34741 agccggtgga tccggccaag gtggaagaca ccttcaaggc ccatcctgag gcctcgctgc
    34801 tggccttcgt ccatgcggag acctccaccg gcgccctgtc ggatgcggcc gcgctggcgg
    34861 ccatcgcgcg ccgcttcggg gccctgacgc tgatggactg cgtcacctcc ctgggcggcg
    34921 tgccggtgtt gctggacgaa tggggcatcg acgccgcgta ctcgggtagc cagaagtgcc
    34981 tgtcgtgcac gccgggcctg tcgccggtga gtttctcgga ggccgccgtg gcgcgcatcc
    35041 gtgcccgcaa gacgccctgc cagagctggt tccaggatct gtccctggtg ctcggctact
    35101 ggagcggcgc gaagcgcacc taccaccaca cggcaccggt caatccgctg tacggtctgc
    35161 acgaatccct gctgatgctc gccgaggaag gcctggaggc cgcctggcag cgccaccggg
    35221 acaaccacct gcgcctgcgc gccggtctgg aagccctggg cctgcgcttt gtggtgcggg
    35281 aagacgcccg cctgccccag ctcaacaccg tgtgggtgcc cgacggcgtg gacgaggccg
    35341 ccgcgcggac ccgcctgctc aaggaatacg gtctggagat cggcgccggc ctcggcgtgt
    35401 tcgccggcaa ggtgtggcgc atcggcctga tgggccattc gagccggccg gccaacatcg
    35461 ccctgtgtct ggcggcactg aaggacgtgc tgggctgatc accctgccca tgtcttgctg
    35521 aaaggtgcgg cggtgtggcc gcccttccgg aggcccagca ccgtgcgacc cggatgacgc
    35581 ggggtggtca gcgcagggat cgcacgtagc ggtccttgtc gccctcgggc gagaccgcgg
    35641 ggctccgcag gcgttcgaag aagccgttgc gtggcgcagg ggcgggcgtc gatggcgtgt
    35701 ctgcctcggc ggcatccaga tccatggcat cgcgcaggcg cccggccagt tccctcagct
    35761 cgtcgtggcc gctggcatcg gccagccgca cgatctcgcg ggcgtagtcc ttgttcgtgc
    35821 gcatgtattc cacgtcggtg atgcggttgg tgacgcgacg catcagcaga tggatgcgcc
    35881 cgagcaggcc gtagaggaca gaatcggatt ccatgggatc tcccggaggc gagcggttca
    35941 gtcgagggct tcgccgtgct cggcgatgtc gaggccgtcc gcctcggtgg tggcgtccac
    36001 ccgcagtccg gtgatcacct ggacgatccc cagcacgatg agggtcatca gcgcactgta
    36061 ggcgaccgtg gcgcccacgc cggccagctg ggcggacatc cggcccgcgc tgccgccgat
    36121 ggccggcgag gccagcaggc cggtgagcag tgccccgatg atgccgccga cgccgtggat
    36181 gccgaaggcg tcgagggcgt cgtcatagcc cagcatgcgt ttcaggctgg tcgacgcgat
    36241 gtagcagccg gcacctgccg ccagtccgat cagaagggcc gacgagggcg acacatagcc
    36301 ggcggccggt gtgatggcta ccagaccgcc cacggcgccg gagacaaggc ccagggcgct
    36361 gggtcgtccg cgcagcatcc attcgacagc catccaggtc accgcggcgg tggcggcagc
    36421 catctgggtg accagcatgg ccatgccggc gtgactgtcg gcggccaggg ccgaaccggc
    36481 attgaatcca taccagccca cccacagcag ggcggcgccg ctcagcgtga gggtcaggtt
    36541 gtggggcgtc atcggctcgc gtccgaatcc ctggcgaggg ccgagtacga gcgcacacac
    36601 cagccctgcc acgccggcgt tgatgtgcac cacggtgccg cctgcataat cgagggcacc
    36661 cagcgcagcc agccagccgc ccggttccca cacccagtgg gcgaccggcg cgtagaccag
    36721 cacggaccac agggcggcga agagcagcac ggctgaaaat ttcatccgct ccgcaaaggc
    36781 accgggcacc agcgcggtgg tgatgatcgc aaaagtgagc tggaacatga catagaccgt
    36841 ttccggcacg gcgccgcccg caaggccctc cgcctgcagc ccttcgagga acaggcggtc
    36901 cagcccgccc aggaaaggcg tgcccggccg cagggccagg ctgtagccgg ccaccatcca
    36961 ggtgagggtg accactgcgg cgatggcaat ggtctgggtc atggtggaca ggacgttctt
    37021 gctccgcacc atccccccgt agaagagggc cacccccggc agactcatga gcaggaccag
    37081 ggcggtcgcc gtgagcatcc aggcggtgtt acccccctcc gcatgggcag cgggcgcgct
    37141 ctgggccagt gccggaccgg aaagggcgag cagcgcggcc atgcccaggg acaggttgac
    37201 cgggcggttt gacgggacta tccggcctgg ggccgatggt cgggtggacg ggtgtgcggg
    37261 catgacatcc tctctgcagg gtatgacgct tctgttgagc gtttgtgcac ccgcagcaag
    37321 agccgtgccg cgctgaaatt ctgcaaatta cggtgtcctt ggcatccgga acgcacccga
    37381 aacgggagcg tcatgctttt cggcacgcag acggtgcatg gggcggtaac cgcccggtcc
    37441 acgaaaaagc ccggccgaag ccgggcttga ggtgctgccg aaggtggcgc cgacgatcgt
    37501 gctcaggcgg ccgccttctc ctcgccggag acgaagaagc tggcgacgta gatcagcaga
    37561 cccaccagga agaccacgcc ggtgccttcg cgcagccagt agaacaggga gatctgttcc
    37621 tgggcggcca tgaaggggat gggcgagctg ctgaagcgtt gcagccacac ctgcaggatg
    37681 ccggcggcgg tcaggaacag ggtgatgaac accatcgcga cggtcatcag ccagaacgac
    37741 cacatttcca tggtctgcgc cttctcgctg ttggcggcac ggccgcgcag gatcggcatg
    37801 gcgtagctga tgatggtgat gaccaccatc gcgtaggcgc cgtagaaggc catgtgaccg
    37861 tgggctgcgg tgatctgggt gccatgggtg tagtagttca ccggtgccag ggtgtgcagg
    37921 aagccccaca cgccggcgcc gaggaaggcc atcacgccgg tgcccagcgc ccacagggtg
    37981 gcggccttgt tggggtgctc gcggcggcgg cggttcacca tgttgaaggc gaagacggtc
    38041 atcgcgaaga acgggatcgg ctccagcgcg gagaacacgg aaccccacca ttgccagtac
    38101 tccggcaggc cgatccagaa gtagtggtgg ccggtgccga tgatgccggt gaccagggtc
    38161 agggtgatga tgacgtacag ccacttctcg atgacttcgc ggtcgacgcc ggtcaccttg
    38221 atcagcacga aggccagcag cgagccgagg atgagctccc agacgccttc gacccacagg
    38281 tgcacgaccc accaccagta gaacttgtcg agcaccaggt tgtgcgggtt gtagaaggag
    38341 aacaggaaga acaccgccag gccccacaga ccgagcatca gcacgatgcc gatggaggtc
    38401 ttgcggccct tgagcatggt catggtcagg ttgaacaaga acaccagcgc gacgaccacg
    38461 atgccgatct tggtcagcag cggttgttcg aggaattccc ggcccatcgt ctcgaggatg
    38521 tcattaccgg tgaacttcgc cagggtggcg tagggcacga gcagatagcc gaggatggtc
    38581 gcggcgcccg ccacgaggaa gacccagaac atgagcagcg ccagttttgg cgagtagagc
    38641 tcggtctcgg tctcttccgg gatcatgtag taggccgcgc ccatgaagcc gaagagcagc
    38701 cagacgatca gcaggttggt gtgaaccatc cgggccacgt tgaacgggat ggcggggaag
    38761 aggaagtcgc cgatcacata ctgcaggccc atgatgaggc cgaacaggat ctgaccgagg
    38821 aacagcccga tggccgcgat gaagtatggc ttcgcgaccg actgtgagga atattgcatg
    38881 gttgatagct ccctttcaga tcggcgatca gccttcgatg ttgggcggcc agttggcagt
    38941 gttgatctcg gacgtgtact tcaggaactc gacgagatcg ttcagctgct ggtcggtcag
    39001 gtgaaagttg ggcatctggc gacggcccgg ggcgcccgtg ggctgggact gaatccacgc
    39061 cttgatgaac tcggggccgc gacgcttgta tacattgccc agttcgggag cgaagtaggc
    39121 accctcgccc agcagggtgt ggcaaccgat gcagttattg acctcccata ccttcttgcc
    39181 cgcagcgaca gccggggtca gattggcccg gttatcccgc ttgggaaggg tctgcatcga
    39241 atcgaaggtc agcgcgatga acaggagcgc aaagaaaaca gtgcccccga agaagatgtt
    39301 tcgcgccatt gattttgtga atgtcccgct catggaacct ccctggatgg cttcgctgca
    39361 cggcgcagct tggccgcgat gttccgcggc cgaggggggc gacgtccttg actcgtatca
    39421 agcaggccgg atggcgtgga atccgctgcg ccggcgatga cgggagaaag cgcccggagg
    39481 ggcctcaggc ggcgttcagg cgcgcgagca gccgacggcc gcgggcctgc atcgccgggg
    39541 taccgcttgc ggcgagcgcc gtcagcaggc ggcgcacctc gtcagtgaag cgcgggtggc
    39601 ggcgggccag atcggccagc gcctgcatcg cctcggtgcg cacgatgcgg ctgggatccg
    39661 cggtgtagcc caccaaaatc gcgtataccc ggtcggcttc ggcggtgctc aagggcaagc
    39721 gcaagagcat cggcgccacg tgccagcgga tctcctgctg gggatcggca gccagcccgt
    39781 ccaggagctg gtcccggaag ggcagcagcc actggggttg gcgggcgctg atcttttcag
    39841 ccgcgtcggc gcaacgcatg cgcagcaccg gatcaggtcg ctgcatgccc tcgaagaggg
    39901 tcgccaacag ggccgggtcg gtcaggacgg cctcgactgc gtccgccacg cgtccgatgg
    39961 aacgccggtc gccacctgag aggctgagga ggagcggcgg gagggcgtct tcggacatgg
    40021 gcggatgaaa cggggaggac cgaagcgcac cagcctagcc cattttccgg ccccatgccg
    40081 gtgacgggtt cagacgctgc tgcaggcgga gggggtccac gccagcttgc cggccagtga
    40141 cacgacttca ccgacgatca gcagcgccgg cgggcgcacg ccggccagat tggccagatc
    40201 gggcaaggtg gcgagggtgc cggtgacgac gcgctgttcc ggcagggtgc ccttttcgat
    40261 cagcgccgcc ggcgtggcgg gatcgcggcc gtgggcgatc agcgcaccgc acagggacag
    40321 gagcatgccc acgcccatgt agatcaccgc ggtctgatgg gggcgcgaca gggcaacata
    40381 gtcgagggag gattcgccgg cgcggcgatg gccggtggca aacaccaccg actgggcgtg
    40441 atcccggtgg gtgaggggaa tccctgcgta ggcagcgatg ccacaggcgg cggtgatgcc
    40501 gggcaccacc tcgaagggaa tccccgcggc gaggagttct tccagctctt cgccgccgcg
    40561 tccgaagatg aaggggtcgc cgcctttcag gcggaccacg tggcggcctt cgcgggccag
    40621 gcggaccagc agctggttga tctgttcctg aggcagggtg tggtgaccgg cctccttgcc
    40681 cacgtagaag cgttcggcgc cgggatgggc cagcgccagc acgccgtcac cgaccaggtg
    40741 atccaccacc agtacgtcgg ccgcggcaat caggcgcgca gcgcgcaggg tgaggagctc
    40801 cggatcgccc ggcccggcac ccaccagtgc aactcgcccg gcggggcgtc cggcggcagc
    40861 ggggcgggcg gcatcgtggc gtccgcctgc gtgacgatgc accagcgcat cgaggaaggc
    40921 gcgcagcgcg ccagcgtcgg acagggcaaa ggcggtgacg ggcggcggca tgacgggact
    40981 ccccgaaaca ggatggacgc aatctatcag ccgggtcccg ctggcgaact tgactcctgt
    41041 caaggaaggg gaatcacccg aaagcgcaac cttcgcgcca agcggaattg gccgctcatc
    41101 gggtatcaac caagggagat tcaagatgaa acaactatgg aagaccggcg ccatggccct
    41161 ggccctgctg ccgctggccg tgtcgcatgc ttttgccgaa gacgcaaaga ccacgaccaa
    41221 cgcgccggag gcgaaatacc agtcggccgc ttcgccgctt gccaacgtgg agatgtacca
    41281 ggatatcaat ccgaaggccc cgccgatgag caaggcggag ttcgacaagg cccggcagat
    41341 ctacttcgaa cgctgtgccg gctgtcacgg cgtgctgcgc aagggcgcca ccggcaagcc
    41401 gctgactccc gagaaaaccc tcgccgccgg caccgattac ctgaaggtct tcatcaagta
    41461 cggctctccc gccggcatgc ccaactgggg cacctccggt gagctgtccg acgacgaagt
    41521 ggacctgatg gcccgctacg tccagcagac cccgccgcag ccgccggaat tcggcctgaa
    41581 ggacatgaag gcgacctgga aggtcatcgt gccgccggaa cagcggccga agaagaagat
    41641 gaacaactac aacatcgaga acatcttctc gaccaccctg cgcgacacgg gtgaagttgc
    41701 actcatcgac ggcgacacca agaagatcat caacatcgtc aagaccggct acgcggtgca
    41761 catctcccgc gtctccgcct ccggccgcta cctgttcgtg atcggccgcg acgccaagat
    41821 caacatgatc gacctgt
//
LOCUS       DAOQPA010000015        41705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_374_length_41705_cov_18.349820, whole genome shotgun sequence.
ACCESSION   DAOQPA010000015 DAOQPA010000000
VERSION     DAOQPA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 41705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41705
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_41705_cov_18.349820"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKX01_03710"
     CDS             <1..406
                     /locus_tag="PKX01_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75259.1"
                     /translation="GALGVAAAAPGAMPAASPATGALAAATSVQAPPEVKAGEEFTVS
                     VGVQAGGNEGGSVDLNFDSRLVESADGPADGDLRKLTLPPGTAGVVFARFRVRPGASG
                     TAVFALGQAQLRVGGQAQMVPPPPPVSVQIQP"
     gene            415..888
                     /locus_tag="PKX01_03715"
     CDS             415..888
                     /locus_tag="PKX01_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ75260.1"
                     /translation="MRRRRGFTLIEMAVTVAVVAVLAAIAAPVVQLSAQRAREAELRT
                     ALRQIREAIDTYKRYSDEGRIAKVVDASGYPPALEDLVKGVPDAKSPKGARLYILRRI
                     PRDPLNPDLSLAADETWGLRSYASPPDEPAEGADVFDVYSRATGKGLNGIAYREW"
     gene            888..1274
                     /locus_tag="PKX01_03720"
     CDS             888..1274
                     /locus_tag="PKX01_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ75261.1"
                     /translation="MEPLSPRRRGFTLVELLVVMAIVAVLLSIAAPRYFGHLGRAREA
                     ALTETLAVTRDAIDKFYGDTGRYPKDLDELVSRRYLRAVPVDPITERRDTWSPVTAPE
                     ALGGGMSDLHSGAEGKTRSGQAYEEL"
     gene            1310..1891
                     /locus_tag="PKX01_03725"
     CDS             1310..1891
                     /locus_tag="PKX01_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ75262.1"
                     /translation="MVYLLTLFAVAALGLGLAQFGQVWAKSVQREREAELLFIGGEFA
                     RALARYKAAGPGVGPAELGDLVTDKRVPVPRRHLRRIYRDPMTGKADWIVERREGQIV
                     GVRSRSEQSVLRRQGLPDWVQVIEVAQRTRGGFRQAPLSLLRGKEADTPSYTDWLFRP
                     KEGPGAAPGAAGRPAAHSAAAPTPPAATAGAVP"
     gene            complement(1904..2929)
                     /locus_tag="PKX01_03730"
     CDS             complement(1904..2929)
                     /locus_tag="PKX01_03730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ75263.1"
                     /translation="MSELVEQAAERLRKAVPIMVKRGIPVTPVNYALWYTYVANENPH
                     FNQHIDDIVATYGTIPPAKAEGLFRDHVSPTGTQENALSKMKSGLERMVADLSRDLYS
                     TLEGTQNFNSLLDECNRGLLAPSGSGSLEEVLGTVERLIKGANAMQQHTSRFESSLST
                     ADEEIRRLRGELATLRKDALHDDLTGVFNRRAFDQELDQFIGQGNMGPSLHLGMLDID
                     HFKAFNDRFGHPVGDRVLQVVGAQLNEMAQVGVSVYRYGGEEFCLIFCGGTLAQAQEQ
                     ADRLRHTIEQLVLKDNRSGERLATITVSIGLASHVYGQSSITLVERADAALYEAKNSG
                     RNRVCIA"
     gene            complement(3433..4716)
                     /locus_tag="PKX01_03735"
     CDS             complement(3433..4716)
                     /locus_tag="PKX01_03735"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ75264.1"
                     /translation="MNLHIQNGRLIDPANRLDTHQDLFIADGKIVGVGSAPAGFSAGR
                     VIDARGLVVAPGLIDLAARLREPGFEYKATLESEMDAAMAGGITSLAIPPDTDPVLDE
                     PGLVEMLCYRAKKLNRAHVYPVGALTIGLKGQQLSEMAELVEAGCVAFSQANVPIVDT
                     RVLYRAMLYAATFGFRLWLQPIDPHLSRGGVAHDGEVASRLGLPGIPAAAETVALYTY
                     LQLARLTGARLHITRLSSADSLDHIEQARRDGVDVTCDVAAHALHLSDMDIGYFNPHC
                     HVIPPLRSQRDRAALSKAVAEGRINAICSDHTPVDDDAKQAPFSESEPGNTGLELLLP
                     LTLKWAGEHGLPLLDALARITSDAAKIAGIAKGGHLSVGARADVCVFDPEAYQLITRS
                     LLKSQGKNTPFLGLELPGKVRYTLVEGSLMYEAAA"
     gene            complement(4727..5686)
                     /locus_tag="PKX01_03740"
     CDS             complement(4727..5686)
                     /locus_tag="PKX01_03740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMZ75265.1"
                     /translation="MRNPQLNRNGELQHLLTLDGLPRAILTQILDTAEPFTEVAEREV
                     KKLPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNVATSSTKKGETLLDTID
                     NLCAMQADMFVVRHESSGAPYLIAQHLQTTGRDHIHVVNAGDGRHAHPTQGLLDMFTI
                     RHYKKEFHNLTVAIVGDVLHSRVARSQIAALTTLGVPEVRVIGPRTLIPTDVEKLGVR
                     YCPTMAEGLKDVDVVMMLRLQNERMNGALLPSPQEFFKIWGLTADKLALAKPDAIVMH
                     PGPMNRGVEIDSAVADGGQAVILPQVTFGIAVRMAVMSMLGSH"
     gene            complement(5679..6179)
                     /gene="pyrR"
                     /locus_tag="PKX01_03745"
     CDS             complement(5679..6179)
                     /gene="pyrR"
                     /locus_tag="PKX01_03745"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMZ75266.1"
                     /translation="MQLPDAEQLIATLAEAMRPQVSPDTALVGIHTGGVWLAQRLHAL
                     LGLSQPLGSIDVSFYRDDYAAKGLHPRPQGSKLPFDVEGTPVIIVDDVLYTGRTTRAA
                     LNELFDYGRPARVDLAVLVDRGGRELPIHPRFCALTLTEPLPSDQNLQLERDEAGALS
                     LRLIHA"
     gene            complement(6166..6594)
                     /gene="ruvX"
                     /locus_tag="PKX01_03750"
     CDS             complement(6166..6594)
                     /gene="ruvX"
                     /locus_tag="PKX01_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMZ75267.1"
                     /translation="MSTVLGFDFGLARIGVAVGETETGLAHPLAVIGDEANAARFAAI
                     EKLLAEWKPATLVVGLPTHLDGSEHAMTARCRRFANQLHGRYGLPVALVDERLSSAEA
                     EARLKEAGHAGWRRQKPLLDATAAQIILSQYLENAAHAAP"
     gene            complement(6591..7157)
                     /locus_tag="PKX01_03755"
     CDS             complement(6591..7157)
                     /locus_tag="PKX01_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ75268.1"
                     /translation="MTKPTINLTDHFLIAMPAMADPNFARTLTYIAEHNENGAMGVIV
                     NRPTDMRLATLMERIDIPFEQPAFADLPVYFGGPVQSDRGFVLHRPAGQWHSSLKVTD
                     DIGLTSSRDVLQALALDGEPAEVLVALGYAGWSAGQLEHELTQNAWLTVPADLTIVFD
                     LPPEERLAAAMQLLGVDFAKLSDVAGHA"
     gene            7294..8736
                     /locus_tag="PKX01_03760"
     CDS             7294..8736
                     /locus_tag="PKX01_03760"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HMZ75269.1"
                     /translation="MTAALVWFRRDLRDVDHAALYHALRRHDRVHCAFVFDTEILDAL
                     PGRMDRRVEFIHGSVVALDAALRARGGGLIVRHGRARDVIPALAASLGVAEVLSNRDY
                     EPAAIARDDEVAARLRGMGIGFADFKDQVIFERGEVLTQGGAPFSVFTPYKRAWLKAL
                     TPFYVKAYPVEAYAGHLAPPPDGTRMPTLAELGFATTNLAELGVTPGRAGGRRLLADF
                     LTRIGRYKSARDFPARKGVSYLSVHLRFGTVSIRELAAAALAVGGEGAETWLSELIWR
                     DFYQQILWHHPRVVAASFRPEYDRVVWDDAPDLFAAWCEARTGYPLVDAAMCQLLHSG
                     YMHNRLRMVVASFLTKDLGIDWRLGEAFFAEHLIDFDLAANNGGWQWAASTGCDAQPY
                     FRIFNPVTQSEKFDPDGAFIRRYLPALARVPARYLHAPWTMPPVDQKLAGCVIGQDYP
                     APVVDHARARARTLARFAVVKGADGDVAEA"
     gene            complement(8741..9034)
                     /locus_tag="PKX01_03765"
     CDS             complement(8741..9034)
                     /locus_tag="PKX01_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMZ75270.1"
                     /translation="MFVVIVTYLRPLSEVDAAVPDHRAFLARHYAAGTFLLSGPQVPR
                     SGGVILARNVSRDALTALLAEDPFSQRGLAEYRIVEFAPNLAAADFAHLLPAD"
     gene            complement(9115..11457)
                     /locus_tag="PKX01_03770"
     CDS             complement(9115..11457)
                     /locus_tag="PKX01_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ75271.1"
                     /translation="MQGSWQVKSLVAALATIGIGSGLTGCGGGGSDVGTSTGVVVGSY
                     FQNAKVCLDENNNAVCDANEFSTRSDAQGRFTLPVAKVPVVAEIGTDASRLDPDTGTA
                     SAVTDKLVFRAPAGNTAVLSALTTLVVAEMDGGASLADARSTVAGRLGVDGAKLMGDY
                     NTETDSLTRSQLLAQGNNAIQTIAATVKTAGSDLKGGMGKTLTAKAVDSKVLYVWTQV
                     GADDTTPFSKVSITGSSSAVALNADPVKGKVADATTYTVSDSSNGSPVSAGALYARAI
                     TTAATCPSITVDGVAQPMAVRAPAITQVDSNGQYSTGSKSVPVKADFAVLTCEFRIPD
                     TARSAIVNGQALKLLNSSTALNRIVVIGDTGCRIKGPTAYTNGAGGDPLQDCTDDRAW
                     PYKRIAAAAASFNPDLVIHNGDIHYREGTPVGVETPAGVAGTGKNEDFLQQKGLMPTV
                     TYGWAAWEADFFKPTGALLSAAPWAITRGNHESCFRAATGWFHFLDPRPFPAATSAAK
                     EPKYSPTYDPSTCSDYIDPVAITLKDLQIVLLDNSGLQDDPGVGKNQGWTHGDHIRTA
                     RQLNALANLSASKDPTKVTWIVTHKPLFAYTGGTGAATPRTWQYQKAIAASGVNGVET
                     YAGGNGSLPANTQMLHAGHIHGWQMISHPAASNLPTSFLIGVSGDTLEAGFMFDSATG
                     APFAPANFGTAITSGVWPWLGAKAALWFGSLAQSLPDAFSTSPIVNANDPAKARITDF
                     GFVVFDRIPGTTNWTSTFYDMDRKKIRACTTVGKRTRCDG"
     gene            complement(11529..12371)
                     /locus_tag="PKX01_03775"
     CDS             complement(11529..12371)
                     /locus_tag="PKX01_03775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ75272.1"
                     /translation="MSHPPGFDARRFKALERSGFNRIAARYADGAPLRADLQDALLAA
                     AALAPGESVLDLAAGPGLLARAAARQVLPGGCVVASDIAEGMLAEGLRRAADEGVPDL
                     LAAACDAEHLSFADARFDAALVGLGLFILPHPERAVAELHRVLRPGGRLAVSVWGPRN
                     DVPLIAHALDCIARHLPAPKVPRPSVFRLGDAAVLHDLLDDAGFRAIAVTPHRLACRF
                     DSADAYWQAFLDLAGGAAEALLRLPDATQATLRAAVADDLAPHRDGAGFTLESTVLIA
                     TARR"
     gene            complement(12400..12969)
                     /locus_tag="PKX01_03780"
     CDS             complement(12400..12969)
                     /locus_tag="PKX01_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HMZ75273.1"
                     /translation="MILYLHGFRSGPQSWKARSLAARMAELGLGDRLWCEQLPVSARA
                     TMALAEAAIAASPRPPTLVGSSLGGYYATYLAEKHGLRAVVVNPAVLAPLSLSAYLGP
                     QTHLYTGETFDFTPAHIDELRALDVPALSHPERFWLLAETGDEVLDYRHAVARYAGCR
                     QTVLDGGDHSFTRWDAYLDPILRFAGLIP"
     gene            complement(12966..13655)
                     /locus_tag="PKX01_03785"
     CDS             complement(12966..13655)
                     /locus_tag="PKX01_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75274.1"
                     /translation="MQHPGSDDGLLQHGSPLALRRAFKAAMNPERLAERADDLRHILD
                     RLGHDPALPDVRAPIGLAGHSFGAVTVQLLAGERRPTQRVVPLDPRVRAILALSPSAR
                     GEGHDLTERFGALERPFFSLTGSRDDGMGLSDITAANRELPFRHMPPGHKYLLVFAGG
                     THLDFAGQSSPAEQGMFRLRRAPPPFQNALLAASTAFWRAHLLDDSDARHWLESGLPG
                     ILRNDDRYARK"
     gene            complement(13918..14742)
                     /locus_tag="PKX01_03790"
     CDS             complement(13918..14742)
                     /locus_tag="PKX01_03790"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ75275.1"
                     /translation="MDIILLVKALILGVVEGLTEFLPISSTGHLILAGDLLNFNDDRG
                     KLFEIVIQSGAILAVVWEFRERLARTFAGLGRQREANRLVLNVAIAFLPLAVLGLAFK
                     KVIEAHLFKPVPVALAFIVGAFVILWAERRDHRIRIHSVDEMRPLDALKLGIAQAFAL
                     IPGTSRSGATIIGGLLFGLSRKAATEFSFFLAIPTLFIATLYNLYKERALLNADDIGM
                     WVVGFIAAFVSAFLCVRWLLRYISHHDFVPFAWYRIFFGLVVLATWHFGLVEWTSS"
     gene            14938..16164
                     /locus_tag="PKX01_03795"
     CDS             14938..16164
                     /locus_tag="PKX01_03795"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HMZ75276.1"
                     /translation="MLKKIPVERLRAGMFIHELCGDWMSHPFWRARFRLNGGDDLQRI
                     VESGIRHVYIDTDKGLDDAEAVDAGAVREAVEAQMVAALATPEETPLRVSVAEEMERA
                     RRVHEQAHRVVRGMMQDVRLGRAVSLEDAEPVVEAITGSVLRNSGALIGLIGIKNKDD
                     YTFLHSVSVCTLMIAFGRSLGLAGEDLRQAGIGGLLHDVGKMKVPDAVLNKPGRLTDA
                     EFALIKMHPGDGHAVLLETPGIGPVPLDITRHHHERLDGSGYPDGQQGEAISSMARMA
                     AIVDVYDAITADRCYHQGLPAAEALRKMWEWSAAHFDQKLLQAFMRCVGIYPVGSLVR
                     LESGRLGVVIDQNEGALLTPRVRVFFSTRSNAHIPPEIVDLGRKPGHGGGDRIVSAEA
                     PDKWGVDPARFLLREG"
     gene            complement(16172..16402)
                     /locus_tag="PKX01_03800"
     CDS             complement(16172..16402)
                     /locus_tag="PKX01_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HMZ75277.1"
                     /translation="MQAPTHTLGHLFEQLGLPADSGAIEAFLDSHSLPPDMRLADAPF
                     WTKPQADFLARELADDADWAELIDSLDARLRH"
     gene            16563..17120
                     /locus_tag="PKX01_03805"
     CDS             16563..17120
                     /locus_tag="PKX01_03805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HMZ75278.1"
                     /translation="MSSPLKIVTLLGSLRQGSYNAAIARALPTVAGEDVVVEALPSIR
                     DIPVYDADLQAEGFPPAVIALADAIRAADGVVIVTPEYNYSVPGALKNAIDWLSRVPE
                     QPFAGKPVAIQTASMGILGGARCQYHLRQVLVFLDCHVLNKPEVMIGAVQDKVNAAGE
                     LADTGTLKRLDEQLLAFTKFIRRLG"
     gene            17117..17695
                     /locus_tag="PKX01_03810"
     CDS             17117..17695
                     /locus_tag="PKX01_03810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ75279.1"
                     /translation="MSLADWVAHYGYFAVAVGCLLEGETVLLLAGVAVHRGLLDLPAV
                     AAVAFVAGFAGDQAWFFVGRRFGDRLIRRFAAVQRAVPRIQDLILRHQNLLVLGIRFM
                     IGLRIAGPVLMGWSRVQALRFVLLNLLGAALWVAVIGGGGYYFSALMERLLPDLRRAE
                     EGLLAATAAAGLVAHGVRLWRGRAARGRNKVY"
     gene            17730..18296
                     /locus_tag="PKX01_03815"
     CDS             17730..18296
                     /locus_tag="PKX01_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMZ75280.1"
                     /translation="MRHMFIAALALSAGIAQAADLDALKSETRQKALPVLPKVAGMMK
                     ETVVAEGTVAAIPVCKEKAPQLLRALSAETGWDIRRVSLKTRNPQRGTPDVWEARQLA
                     DFNIRAANGEKVETLETGEIVSEHGKPVYRYIRAIPAGDVCLACHGAADTLDAPLREQ
                     LARHYPDDRATGYTKGQIRGALTVKRPL"
     gene            18428..18784
                     /locus_tag="PKX01_03820"
     CDS             18428..18784
                     /locus_tag="PKX01_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75281.1"
                     /translation="MNKSLFALSLALALGAAHAADVKPADPRATPADAVKAGAGAAAA
                     GQTAMEQHVRTHAAGKAEAVKRAAVPREAQASAAVRVKDAVAPQGARMDGAKAAVKAE
                     AKPADQAAALAGAVVK"
     gene            19036..19377
                     /locus_tag="PKX01_03825"
     CDS             19036..19377
                     /locus_tag="PKX01_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75282.1"
                     /translation="MTLRTASLLFALLSSTAHACGPYEDDWAGRDKAAHFGVSAALGV
                     ATSQISSDPWTAFALALAPGVAKEVYDSQRACNRFSWKDLSWDAAGALVGVKTGHWLL
                     SPGGVAYRRSF"
     gene            complement(19399..19950)
                     /locus_tag="PKX01_03830"
     CDS             complement(19399..19950)
                     /locus_tag="PKX01_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75283.1"
                     /translation="MPATAPAFRPPPILDIEASGFGPSSYPIEVGLILPDGCTYCSLI
                     RPLDAWTHWDASAEAIHRVARSTLVAHGRSPADVAHALNLRLHGQTIYCDAWYHDFTW
                     LGRLFDAADLPQLFRLEDIRSLLGETDMEVWHATKTDILAELGLQRHRASNDARVLQQ
                     TLLRVKGWTAPIPAEAQPCDRPV"
     gene            20080..20682
                     /locus_tag="PKX01_03835"
     CDS             20080..20682
                     /locus_tag="PKX01_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ75284.1"
                     /translation="MPIDPSATFPVHPRPWAGALLRRLGWTLDVAPLPAAHCLIVVYP
                     HTSNWDFPLGLLVRFALGWPIGWIGKHTLFRWPVSGLLRRLGGIPVDRGQPGGFVEGM
                     AGAFRSRDHLLVAIAPEGTRRRTAGWKSGFYRIARAADVPVGLGFIDYRRKRLGIAAY
                     VRLTGNVAADMARIREVYAGMCGYRPDQASPVVLPESERL"
     gene            20757..21962
                     /locus_tag="PKX01_03840"
     CDS             20757..21962
                     /locus_tag="PKX01_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75285.1"
                     /translation="MSESDLSRLSIDRNAPRPSRRRARAWLIGGALVVVAALAAAIGH
                     FNAPVSVETVSVGEAWPYQGVTVLNATGYVVAQRKASVASKATGRLEWLGVREGSVVK
                     AGELIARLEQRDVVAQAEQAAANVGVARANVGQARAELKDAELALGRARDLRARNFVA
                     QAQVDQAEARATKARAALGSAQAAETAAVAAERAARVAVAQTEIRAPFDGVVLTKNAN
                     VGDLITNFSAAADSKGAVVSMADMGTLEVEADVSEANLGRIRVGQPCEIQLDAFPEQR
                     FRGEVSRRVPTVDRAKASVLTKVRFLDPDPRVLPEMSAKIAFLDKALADAERTPRVAV
                     HEDALRREGSEVALFRVEDGRAHRVVVQTGATIGELVAVQGVKSGDKVVLRPPESLRD
                     GAAVKAGGK"
     gene            21959..22678
                     /locus_tag="PKX01_03845"
     CDS             21959..22678
                     /locus_tag="PKX01_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75286.1"
                     /translation="MTDARGSAAGAPIVRLRDVRKAYRRGAQVVPVLAGIGFDIAEGE
                     FLALMGPSGSGKSTLLNLIAGIDRADSGELIVAGEDIARLAERELADWRARHIGFIFQ
                     FYNLMPVLDALENVELPLLLTTLSARERRERAAFALSMVGLADRMDHTPGELSGGQQQ
                     RVAIARALVTDPTLIVADEPTGDLDRESATEILGLLQRLNREAGKTIVMVTHDQRAAA
                     AAHAIMHLEKGELSGREAVRT"
     assembly_gap    22692..22791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22859..23302)
                     /locus_tag="PKX01_03850"
     CDS             complement(22859..23302)
                     /locus_tag="PKX01_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ75287.1"
                     /translation="MATTVQQILNNKGGTCHGVAPDTTVLEALKLMAERNISAVLVTE
                     GPRLVGIFTERDYARKVVLRGLASKDTKVGDLMTQNVLTVSPSQTVEEVMGIMTNNHI
                     RHLPVVDHGVLAGIISIGDAVKSVMEQQQATIEQLAGYIAGGMST"
     gene            23452..24615
                     /locus_tag="PKX01_03855"
     CDS             23452..24615
                     /locus_tag="PKX01_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75288.1"
                     /translation="MLTAFLRMILRNAWRHRLRAGLTVLGMVVAILAYGLLGTVVDAW
                     YAGAEGASANRLITRSSISLVFPLPLTYRDRIRGVEGVRDLSWASWFGGIYKEPKNFF
                     AQFAVDGESYFRIYPEFVVDERAFRDFLRDRRGCVVGRKLAETYGFKVGDTLTLKGTI
                     YPGNWDFVVRAIYDGARKGTDQSQMLFHWQYLDETIKQRYPGRANNVGVFVTEVASPD
                     QVAAVSRAVDREFANSRAETLTETEKAFQLSFVAMTEAIVVAIRIVSYLVIFIIMAVM
                     ANTMAMTARERTAEYATLKALGFAPAFVGLLILGESLMLALTGGAIGIALTFPVADAF
                     AAKMGTLFPVFNVSTETVALQIACALTVGMVAALLPMLQVGRLRIVDGLRAVG"
     gene            24619..25785
                     /locus_tag="PKX01_03860"
     CDS             24619..25785
                     /locus_tag="PKX01_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75289.1"
                     /translation="MKRIPLAYSLRNLWARRLTTALTAGGMALVVFVFAAVLMLDQGL
                     KEALVDTGQADNVVVIRKGSETEVQSIIERNQAALLETLPQVALAAGGLPQVSKETVV
                     LITLPKHGGKPANVIIRGVGGQGAALRPTLALVEGRMFRPGSAEIVVGASIARRFDGT
                     GLGSTLRFGARDWRVVGVFEADKSGFESEIWGDADQLMQAFRRPAYSSVVLRLADPAS
                     FPAVRALIDDDPRLSLEAKTERAFYADQSAMLSNFISILGMTLSVIFSVGAMIGAAIT
                     MYAAVATRTAEIGTLRALGFRRGSVLLAFLTESLLLALLGGAAGLAAASLMQFFTIST
                     MNWQSFSELAFSFRLTPAIVVKTLIFALAMGLIGGFMPAARAARMNIVSALRDA"
     gene            25958..26437
                     /locus_tag="PKX01_03865"
     CDS             25958..26437
                     /locus_tag="PKX01_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75290.1"
                     /translation="MYVALFCLIGIVALAAISAQAWRDRARVAASVTPDWHDTLRRLA
                     TEQELGHYRSAREKTVSVLRRLESRRFDPRMAAHVRVRIASLMARDPVYTEVVRAIRK
                     ACAAQTSISEMALCVGLRQFLIEDIRISMEIAEMLGQVRRAEEGGSPTVMAEFHPTA"
     gene            complement(26501..27325)
                     /gene="panC"
                     /locus_tag="PKX01_03870"
     CDS             complement(26501..27325)
                     /gene="panC"
                     /locus_tag="PKX01_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMZ75291.1"
                     /translation="MQIHPTIAGLRAALAGAGRVAFVPTMGNLHDGHIALMREARRHA
                     DVVVASIFVNRLQFGPREDFDKYPRTFADDCARLTDAGVDHLFAPDETEMYPAPQRYH
                     VEPEPAQVSILEGAVRPGHFRGVATVVLKLFNIVQPQVALFGKKDYQQLMVLTNMTRE
                     LALPIRIVPGETIRAADGLALSSRNGYLSAAERAEAPRLFAQLSRIRERLQAGERDLA
                     RMEADAVAELAAHGWQPDYIAIRRRSDLQVPEGDEPLVVLAAARLGSTRLIDNLEL"
     gene            complement(27333..28151)
                     /gene="panB"
                     /locus_tag="PKX01_03875"
     CDS             complement(27333..28151)
                     /gene="panB"
                     /locus_tag="PKX01_03875"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ75292.1"
                     /translation="MTYLQDTAKPVTLFELGKMRAEGRRIVMLTGYDASFASLLERCG
                     VDVILVGDSLGNVLQGQKTTLPVTMEHMVYHTECVARGCSRPFIVADMPFGSYAEGPE
                     QAIRNAARLMAAGAQMVKLEGGAVMAETVRFLVERGIPVCAHIGLTPQSVHQLGGYRV
                     QGRSDSGAATLKADAFALEQAGAALMVLEMIPAALATDLTRSLTTMATIGIGAGVDCH
                     GQVLVLHDLIGVFPGRRPRFAKNFMEGAASLDDAVTRYVTDVREGRFPGPEHSY"
     gene            complement(28276..28914)
                     /locus_tag="PKX01_03880"
     CDS             complement(28276..28914)
                     /locus_tag="PKX01_03880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ75293.1"
                     /translation="MLEKARYIVVEGPIGAGKTTLAKRLAERLHADLLLENPATNPFL
                     GRFYQDAERWGLATQIAFLFQRSEQLALFTRDHDPFRRAVSDFLLDKDPLFAELNLDV
                     DELQLYRQLFERLKPTVRQPDLVIYLQAQPDILIERVRKRGVEAERKISESYLQRVAE
                     HYARFFHHYDAAPLFIVNAESLNPVDKDEDFELLYSRLLAMRSYREFFGYAG"
     gene            complement(28930..29271)
                     /locus_tag="PKX01_03885"
     CDS             complement(28930..29271)
                     /locus_tag="PKX01_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HMZ75294.1"
                     /translation="MPIWLQTALLLAASNVFMTFAWYGHLKNLASAPWYIAALVSWGI
                     ALFEYLLQVPANRIGARQLDLPQLKILQEAITLTVFVPFAVFYMQQPLKLDYLWAALC
                     ILGAVYFIFRT"
     gene            complement(29271..29765)
                     /gene="folK"
                     /locus_tag="PKX01_03890"
     CDS             complement(29271..29765)
                     /gene="folK"
                     /locus_tag="PKX01_03890"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMZ75295.1"
                     /translation="MSAGTRAYVALGANLGDPVPTLRAAVADLGALPGTSVVIRSSLY
                     RSAPVGLVNQPDFFNAVVAVDTSLPALDLLRGLLAVEARHGRVRSVPNAPRTLDLDLL
                     LHGDQACSSAELILPHPRMHERAFVLLPLLEIAPDVWIPGRGPAREALPAVAGQAIAR
                     AEAL"
     gene            complement(29774..31108)
                     /gene="pcnB"
                     /locus_tag="PKX01_03895"
     CDS             complement(29774..31108)
                     /gene="pcnB"
                     /locus_tag="PKX01_03895"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HMZ75296.1"
                     /translation="MIRKLLRRVFRRAAPANALPEPAVIPAARHGIRRDQLSPAAIKV
                     CTVLQDAGHKAYVVGGAVRDLIVGQTPKDFDVATSATPEEVRALFRRSRIIGRRFKIV
                     HVMSGPETIEVSTFRAMQDAESTETDEHGRVLRDNVFGSMAEDATRRDFTVNALYYDP
                     SDETVVDYHHGVADLQQRTLRMIGDPRVRYREDPVRMLRAARLGAKLGLGIDPAARGP
                     IREMADLLENVPPARLFDEMLKLLLSGHAVKCLKQLREEGLHHGLLPLLDVILEQPLG
                     ERFVWLSLENTDERVRQGKPVSPGFLFATLLWHEVLADWEARKADGQHAQPALFEAMD
                     AVLDKQSGKLAITRRIVGDIKDIWALQPRFDKRAGKAPYRLLEQARYRAGWDFLRLRA
                     QAGEIPMELPDWWDAFADAHPDAREAMLLPEKAGSAPRKRRRRKKPAAAISE"
     gene            31241..32026
                     /locus_tag="PKX01_03900"
     CDS             31241..32026
                     /locus_tag="PKX01_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HMZ75297.1"
                     /translation="MLNFSANLSFLFLERPFLERFEAAAACGFRGVEFHFPYAFPVDE
                     VVDAARRAGVEVVLFNVPAGDWAGGERGLGCLPERRTEFRDGVGLAATYAAALACPRV
                     NVLAGLRPSGVADAVLRATLIDNLRYAADRLSESGRTVLLEPLNTRDTPGFFVSRSHD
                     GLALLAEAGRPNLRLQYDVYHAQVMEGDLARTLGEHLAQIGHIQLADNPGRHQPGTGE
                     IRFPFLFGHLEALGYAGWIGCEYVPTGRSEDSFAWREDWVDSG"
     gene            32215..32952
                     /locus_tag="PKX01_03905"
     CDS             32215..32952
                     /locus_tag="PKX01_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HMZ75298.1"
                     /translation="MPLTPVLRPGRLAEEVGADLERRIRRGDFPPGGRLPSESQLAAQ
                     FGVSRTVVREAIARLRAEGLVDSRQGAGAFVMQRPGAASFRLRDVSAEPRSDAEVFEL
                     RLVVEAAVAERAARRHTAADLAAIAGALARMDAALRAGEGGAEADDAFHRAIAAACGN
                     ALLQRLVEFVSQDLSASRRPTWSRDGLIAGRALRAQDEHRALFEAIAAGDGAAARRAA
                     EQHLIGTAARLGVSWSLDPASPSKEPT"
     gene            32949..34466
                     /locus_tag="PKX01_03910"
     CDS             32949..34466
                     /locus_tag="PKX01_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ75299.1"
                     /translation="MTADAHTFPDFSERERDFSRVDKHRLVTALSRALPAHGLLHEPE
                     DLRPYECDGMSALRQLPLVVALPDTEAQVVEILRICAAQQVPVVARGAGTGLSGGAQP
                     HRFGVVLSLARFKKILHIDAHARRAVVQPGVRNLAISEAAAPYGLYYAPDPSSQIACT
                     IGGNVAENSGGVHCLKYGLTVHNLLKVRAVTIDGEIVEFGSLALDAPGYDLLPLVTGS
                     EGMLAVVTEVTVKLTPKPQLARCVLAAFDDVVKAGDAVARIIAAGIIPAGLEMMDRPA
                     TAAVEEFVHAGYPLDAEAILLCEADGTPEEVAEEIERVRAILEGSGATEVRVSRDEAE
                     RLRFWAGRKAAFPAAGRISPDYYCMDGTIPRKRLGEMLRAIQAMEKKYGMRCINVFHA
                     GDGNLHPLILFDANTPGDQDVAEAFGAEILELSVALGGTITGEHGVGIEKINQMCSQF
                     ATPEIRLFHRVKAAFDPLGLLNPGKAIPTLNRCAEYGRERMGRGVPDAHPDLPRF"
     gene            34472..34639
                     /locus_tag="PKX01_03915"
     CDS             34472..34639
                     /locus_tag="PKX01_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75300.1"
                     /translation="MDALPEGWLQFLFVAVPVLSLIGTWMGLRTAGRIVERRERARRV
                     GQHPGSGDTCD"
     gene            34632..35696
                     /gene="glcE"
                     /locus_tag="PKX01_03920"
     CDS             34632..35696
                     /gene="glcE"
                     /locus_tag="PKX01_03920"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HMZ75301.1"
                     /translation="MTDPIQEWAARIRKAAADGSAVCLRGGGTKDFLGRTPMGEVLDT
                     RGYTGIVSYEPSELVVTARAGTPLVELEAALAEAGQMLAFEPPRFAPGGTVGGCVAAG
                     LAGPRRAAVGGVRDFVLGVRLLDGRGDDLRFGGQVMKNVAGYDVARVMAGSLGTLGLL
                     LEVSLKVLPRPPAEATLAFALDEPAALERVNAWSGQPLPVSATAWRAGRLHVRLSGAA
                     AAVEAAIRRLGGEVLADTAAHGLWAALRDHTDDFFTDDRPLWRLSVPSDAPPLDVPGE
                     TLVEWGGAQRWLKGEQSAAELRARVAAVGGHATLFRHGDRAGEVFHPLLPAMAALQRR
                     LKQTFDPAGVFGPGRLYGGM"
     gene            35700..36956
                     /gene="glcF"
                     /locus_tag="PKX01_03925"
     CDS             35700..36956
                     /gene="glcF"
                     /locus_tag="PKX01_03925"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HMZ75302.1"
                     /translation="MQTQLADFIRDTPEGREAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGQPATARTRLHLDRCLTCRACETTCPSGVEYGRLADIGRLVV
                     ERQVPRQGVDRLGREALRQLVPNTGLFGLALGLGRRARPLLPRLLADKVSAADPAGEW
                     PAARHPRRMIVLAGCVQPSLRPAINAAAARVLDRLGVSLVEQAGAGCCGAVKFHLHDQ
                     EGARMEARRNIDAWWPGVAAGRVEAIVMTASGCGTQVKDYGHLLQHDPAYAEKAVRIA
                     ALCRDLSEVLAAEGDALETLIAGSAAAVERRRIAFHAPCSLQHGQKIRGVVEKLLEAA
                     GFELAPVRDAHLCCGSAGTYSLLQPTLAGRLRADKLDALVDSGGATVVSANIGCIEHL
                     AAGGRFPVKHWIELIDEYFTALRQKI"
     gene            37137..37967
                     /locus_tag="PKX01_03930"
     CDS             37137..37967
                     /locus_tag="PKX01_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ75303.1"
                     /translation="MLEQSLASIPAPDTVTAKKRYQAEACVAKHVGDRTEQQDRVAVF
                     AHPARKGTLLAVLADGMGGHSGGAMAAEQVVLKAKQNFETFAPASESPEDLLRSVVDE
                     AHIVIKLTRFTSEQDPHSTAVVFMMQPDRADWAHCGDSRFYHFRNGKLMTRSADHSLV
                     EELVRKGRLDAESAARHPNRNVLLSCLGADREPRVDLGHVSPLQGGDAFLLCSDGLWG
                     YISEDEFGDVLASHTPRDAASTLIEMARHRARGGGDNISLAIVKLVELPPKTVPPRES
                     "
     gene            complement(37990..38991)
                     /gene="corA"
                     /locus_tag="PKX01_03935"
     CDS             complement(37990..38991)
                     /gene="corA"
                     /locus_tag="PKX01_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ75304.1"
                     /translation="MKTEKPSITLLEYDSTGISEIRFDSIEESRNFTRSREKIWLNVY
                     GLHDPAIMAEIGRRFRLHPLIQEDILNTQQRPKVDEFAEYLYLVARYFDYDAPARCLT
                     SDQISIVLGRNFVLTFQERATGAFEPVRERLRAETGAMRAHGPDYLAYALIDTLVDRY
                     FIMLDHLSDLAEELEESALTRPTPSLLAEINRVKHEARSMRRAVWPLREVLNTLSRAD
                     NDFFTADTRLYLRDIYDHTVHVLESLESVRDLLADLLDIYLSSVSNRLNTEVRILTVL
                     TTLFMPATLIAGIFGMNFHEMPLLADADGFWVAMGLMGGAAAFMAASFWRRNWLKTQ"
     gene            39235..40359
                     /locus_tag="PKX01_03940"
     CDS             39235..40359
                     /locus_tag="PKX01_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ75305.1"
                     /translation="MPLVIGVTKEFTPGERRVALVPDVAKKYQGLGASLLLESGAGEP
                     AYFRNSDYAGSDVADDAAAVLRKADVVLQVQPLGLDALRELRPGSVLVGLQQPWSSAE
                     RIAVLRERNITCFALELLPRISRAQSMDVLSSQAAVAGYECALIAADHCPKFFPMLTY
                     AAGTIRPAKVLVIGAGVAGLQAIATAKRLGAMVSGYDVRPETKEQIESLGAKFVDTGV
                     SASGSGGYARELTDEEKKQQAEKLGKAIGECDALITTAAIPGKKAPVIVSAEMVARMK
                     PGSVVVDMAAETGGNVAGTVPGEKTWVGEVLIIGPTHIPSRMPVHASEMYAKNLFNFI
                     SPFIKDGALALDWEDEVMAGACLTHAGELRHAGVKQALGL"
     gene            40373..40657
                     /locus_tag="PKX01_03945"
     CDS             40373..40657
                     /locus_tag="PKX01_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ75306.1"
                     /translation="MDGFVALYIFMLAAFTGYEVISRVPVILHTPLMSGSNFVHGVVL
                     VGGMIALGQAQTPLEQAIGFVAVLLGAANAAGGYVVTERMLAMFKSKEKN"
     gene            40657..>41705
                     /locus_tag="PKX01_03950"
     CDS             40657..>41705
                     /locus_tag="PKX01_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ75307.1"
                     /translation="MAIKWLIDASYFVAAVLFILGLKGMSSPVTARKGIVWAGVGMVL
                     ATVVTFATPGMQNYLLMVIAIAVGGALAWISGKKVAMTDMPQMVAIYNGMGGGAAAAI
                     AAVEFARGEMHGAVVTLLATLGALIGAVSFAGSVVAWAKLQGVMKKAFRLPAQNAVNV
                     LLALLTVGLGAAIVIAPAVDPTLVGAFFILALILGTIITLPIGGADMPVVISLFNAFT
                     GLAVGFEGFVMGIPALIIAGIVVGAAGTLLTQLMAKAMNRPISNILFVPMAASGGGEA
                     VEGEQKEVGAMDAAAMMRYASKVIIVPGYGMAVAGAQHKVWEMTELLEEAGVEVSFAI
                     HPVAGRMPGHMNVLLA"
BASE COUNT         6171 a        12780 c        15751 g         6903 t
ORIGIN      
        1 gggcgcgctg ggggtggcgg ccgcggcacc gggtgcgatg ccggctgcgt cgcccgccac
       61 cggggcgctg gcggcggcca ccagcgtgca ggctccgccg gaagtgaagg cgggcgagga
      121 attcacggtg agcgtcggcg tgcaggccgg cgggaacgag gggggcagcg tggacctcaa
      181 tttcgacagc cgcctcgtgg agtcggccga tgggccggcg gacggggatc tgcgcaagct
      241 gaccctgccg ccgggcaccg cgggcgtcgt gttcgcgcga ttccgggtgc ggcccggcgc
      301 cagcggcacg gcggtgttcg ccctggggca ggcccagttg agggttggcg gccaggccca
      361 gatggtgccg ccaccaccgc ccgtgtcggt gcagatccag ccttgaggca cggcatgcgg
      421 cgccggcgcg gctttaccct catcgagatg gcggtcaccg tggcggtggt ggcggtgctc
      481 gccgccatcg ccgcgccggt ggtgcagctc tcggcccagc gggcgcggga ggccgaactg
      541 cgcacggccc tgcgccagat ccgcgaggcc atcgacacct ataagcgcta cagcgacgaa
      601 gggcgcatcg ccaaggtcgt ggatgccagc ggctatccgc ccgcgctgga ggatctggtg
      661 aagggtgtgc ccgacgccaa gtcgccgaag ggcgccagac tctacatcct gcgccgcatt
      721 ccccgtgatc cgctgaaccc ggacctgagc ctggcggcgg acgagacctg gggtctgcgc
      781 agctacgcca gcccgccgga cgagccggcc gagggtgccg acgtcttcga cgtgtactcc
      841 cgggccaccg gcaagggcct caacggcatc gcctaccggg agtggtgatg gaaccacttt
      901 ccccacgtcg gcgcggcttc accctggtcg aactgctggt ggtgatggcc atcgtggcgg
      961 tgctgctgtc catcgccgcg ccgcgctatt tcgggcatct ggggcgggcc cgtgaggcgg
     1021 cgctcaccga gaccctggcg gtcacgcggg acgccatcga caagttctat ggcgacaccg
     1081 ggcgctatcc caaggatctg gacgagctgg tgagccggcg ctacctgcgc gcggtgccgg
     1141 tggatcccat caccgaacgt cgcgacacct ggtcgccggt gactgcgccg gaagccctcg
     1201 gcggcggcat gagcgacctg cacagcggcg cggagggcaa gacccgcagc ggtcaggcct
     1261 acgaggagct atgagggcag gacgcgcccg gcgccggccc cagtctggcg tggtctatct
     1321 gctcaccctg ttcgcggtgg cggccctggg cctggggctt gcccagttcg ggcaggtgtg
     1381 ggcgaagagc gtgcagcgtg agcgggaggc ggagctcctc ttcatcggtg gcgagtttgc
     1441 ccgcgccctg gcgcgctaca aggcggccgg ccccggcgtg gggcccgcgg agctgggcga
     1501 tctggtgacg gacaagcggg tgcccgtacc gcgtcgccat ctgcggcgga tctaccgcga
     1561 tcccatgacg ggcaaggcgg actggattgt ggagcggcgc gaggggcaga tcgtgggcgt
     1621 gcgcagccgc tcggagcagt ccgtgctgcg ccggcagggg ctgcccgact gggtccaggt
     1681 catcgaagtg gcgcagcgga cgcggggcgg tttccgccag gcgcccctga gtctgctgcg
     1741 cggcaaggag gcggacacgc caagctacac cgactggctg tttcgcccca aggaagggcc
     1801 gggcgctgcg ccgggtgcag cgggtcgtcc ggcagcccat tcggccgcag cgccgacgcc
     1861 gccggccgcg accgccggcg ccgtgccctg acgcggcgtc gcgtcaggcg atgcagaccc
     1921 ggttgcggcc gctgttcttc gcctcataca gggccgcgtc ggcccgttcc accagggtaa
     1981 tcgaggactg cccgtagacg tggctggcca gcccgatgga caccgtgatg gtggccagcc
     2041 gctcgccgga ccggttgtcc ttgagcacca gctgctcgat ggtgtggcgc agccggtcgg
     2101 cctgttcctg ggcctgggcc agggtgccgc cgcagaagat caggcagaac tcctcgccgc
     2161 cgtagcgata caccgacacg cccacctggg ccatctcatt gagctgggcg cccaccacct
     2221 ggaggacgcg gtcacccacc gggtggccga aacggtcgtt gaaggccttg aagtggtcaa
     2281 tgtccagcat gcccaggtgc agcgacggtc ccatgttgcc ctggccgatg aactggtcca
     2341 gttcctggtc gaaggcgcgg cggttgaaga cgccggtgag gtcgtcgtgg agggcgtcct
     2401 tgcgcagggt ggcgagctcg ccgcgcaggc gccggatctc ctcgtcggcg gtgctcaggg
     2461 agctctcgaa acgcgaggtg tgctgctgca tcgcgttggc gcccttgatg aggcgctcca
     2521 ccgtccccag cacctcttcc agactgccgg atccggaggg cgcgagcagc ccccggttgc
     2581 attcgtcgag cagggaattg aaattctggg tgccttccag cgtggagtag aggtcgcggc
     2641 tgaggtcggc caccatgcgc tcgaggccgg acttcatctt ggagagggcg ttctcctggg
     2701 tgcctgtcgg tgagacatga tcgcggaaca ggccttcggc cttggccggc ggaatggtgc
     2761 cgtaggtggc gacgatgtcg tcgatgtgct ggttgaagtg ggggttttcg ttggccacgt
     2821 aggtgtacca gagggcgtaa ttcaccggcg tgacggggat gccgcgcttc accatgatgg
     2881 gcacggcctt gcggaggcgt tcggctgcct gttcgacgag ctcactcatt cttgttcttc
     2941 ccttgtgttc tggactcccg cggacctccc caaaagagac gaggtccggt ccgccagtgc
     3001 gaccaccgat cggcgcttgc ccggctgaca tcaggaaata tcggtcaccg ggggagaagt
     3061 tgagcgggtt ctgcaacaat gtgtgaatta attggagacg tcggaagaag gcgtttttca
     3121 gtgagaaaaa atctataaaa aacaaatgcg tactgtcact ttccggcggg ggtgcctacg
     3181 gctttggata tagcttaggc gaatgggatt ttgatgaatg atgcattttt cgagggcttg
     3241 gcggattcgg aggattttgg cggtcaggcc gctgcgcaat gcgtgggacg gggctgaggt
     3301 ggcgactcgg ggcgggcacg cggaccgcgc cgagtcggcg cgtccttcgg gggcgggggc
     3361 gtgggttcgg cggggcgaaa aagcgacggc gcccgcgggc gccgtctgga acatgggatg
     3421 ggttgggccg gatcaggcgg ccgcttcgta catcaggctg ccttccacca gggtgtagcg
     3481 caccttgccg ggcagttcga ggcccaggaa gggggtgttt ttgccctggc tcttgaggag
     3541 actgcgggtg atgagctggt aggcctccgg atcgaagacg cacacgtcgg cgcgggcgcc
     3601 tacggacagg tgcccgcctt tggcgatacc ggcaattttt gccgcgtccg aggtgatgcg
     3661 ggccagggca tccagcagcg gcagtccgtg ttcgccggcc cacttgaggg tgaggggcag
     3721 gagcagttcg aggccggtgt tgcccggttc ggattcgctg aagggggcct gcttggcgtc
     3781 gtcgtccacc ggcgtgtggt cggagcagat ggcgttgatg cggccttccg ccaccgcctt
     3841 ggacaaggcc gcccggtcgc gctggctgcg caggggcggg atcacgtggc agtgggggtt
     3901 gaagtagccg atgtccatgt cgctcaggtg cagggcatgg gcggccacgt cgcaggtgac
     3961 gtccacgccg tcccgccggg cctgctcgat gtggtcgagg ctgtcggcgc tggacaggcg
     4021 ggtgatgtgc aggcgggcgc cggtgaggcg ggcgagctgc aggtaggtgt acagggccac
     4081 ggtctcggcg gcggcgggga tgcccggcaa accgaggcgc gaggcgacct cgccgtcgtg
     4141 ggcgacgcca ccgcgggaca ggtgcgggtc gatgggctgc agccacaggc ggaagccgaa
     4201 ggtggcggcg tagagcatgg cgcggtagag cacgcgggtg tccacgatgg gcacgttggc
     4261 ctggctgaag gcgacgcagc cggcttcgac cagctcggcc atctcggaga gctgctgtcc
     4321 cttgaggccg atggtgaggg cgccgaccgg gtacacgtgg gcgcggttga gcttcttggc
     4381 ccgatagcag agcatctcga cgaggccggg ttcgtccagc accgggtcgg tgtcgggcgg
     4441 gatggcgagg ctggtgatgc cgccggccat ggccgcgtcc atttcggatt cgagggtggc
     4501 cttgtattcg aagccgggtt cgcgcaggcg cgcggccagg tcgatcaggc cgggggcgac
     4561 caccagtccg cgggcgtcga tgacgcggcc ggcgctgaag ccggcgggcg cgctgccgac
     4621 gccgacgatc ttgccgtcgg cgatgaagag gtcctggtgg gtgtcgaggc ggttggccgg
     4681 gtcgatgagg cggccgttct ggatgtgcag gttcatcgtg gtggtctcag tggctgccca
     4741 gcatgctcat cacggccatg cgcaccgcga tgccaaaggt gacctggggc aggatcaccg
     4801 cctgcccgcc gtcggccaca gcggagtcga tctcgacgcc gcggttcatc gggccggggt
     4861 gcatgacgat ggcgtcgggc ttggcgaggg ccagcttgtc ggcggtgagg ccccagatct
     4921 tgaagaattc ctgcgggctg ggcagcaggg cgccgttcat gcgctcgttc tgcaggcgca
     4981 gcatcatcac cacgtccacg tccttgaggc cttccgccat ggtcggacag tagcgcacgc
     5041 cgagcttttc cacgtcggtg gggatcaggg tgcgggggcc gatgacgcgc acttcgggca
     5101 cgcccagggt ggtcagggcg gcgatctggg agcgcgcgac gcggctgtgg agcacgtcgc
     5161 cgacgatggc gacggtgagg ttgtggaatt ccttcttgta gtgccggatc gtgaacatgt
     5221 ccagcaggcc ctgggtgggg tgggcatggc ggccgtcgcc ggcgttgacc acgtggatgt
     5281 ggtcgcgtcc ggtggtctgc aggtgctggg cgatgaggta gggggcgcca gaggattcgt
     5341 ggcgcaccac gaacatgtcg gcctgcatgg cgcagaggtt gtcgatggtg tccagcaggg
     5401 tctcgccctt tttggtggac gaggtggcga cgttgaggtt gatgacgtca gcggacaggc
     5461 gcttggcggc gatctcgaag gtggtgcggg tgcgcgtgct gttctcgaag aacaggttga
     5521 agacgctctt gccgcgcagc agggggagct tcttcacttc ccgctcggcc acttcggtga
     5581 agggctcggc ggtgtccaga atctgggtga ggatggcgcg gggcaggccg tcgagggtga
     5641 gcaggtgctg cagttcgccg ttgcggttga gttggggatt acgcatggat cagcctcagg
     5701 gagagggcgc cggcttcgtc gcgctccagt tgcaggttct ggtcggacgg cagcggctcg
     5761 gtcagggtga gggcgcagaa gcgcgggtgg atgggcagtt cgcggccgcc ccggtccacc
     5821 agcacggcca ggtcgacgcg cgccgggcgg ccgtagtcga agagttcgtt gagggccgcg
     5881 cgggtggtgc ggccggtgta gagcacgtcg tccacgatga tcaccggcgt gccctccacg
     5941 tcgaagggca gcttggagcc ctggggacgc gggtgcaggc ccttggcggc gtagtcgtcc
     6001 cggtagaagc tcacgtcgat gctgcccagg ggctgggaca ggccgagcag ggcgtgcagg
     6061 cgctgggcga gccacacgcc gccggtgtgg atgcccacca gggcggtgtc gggcgagacc
     6121 tgggggcgca tcgcctcggc cagggtggcg atgagctgtt cggcgtcagg gagctgcatg
     6181 ggcggcgttc tccaggtact gggacaggat gatctgggcg gcagtggcgt cgagcagggg
     6241 tttctgccgg cgccagccgg catggccggc ttccttgagg cgcgcttcgg cctcggcgga
     6301 gctgagccgc tcgtccacca gggccaccgg caggccgtag cggccgtgga gctggttggc
     6361 gaagcgccgg cagcgggcgg tcatggcgtg ttcgctgccg tccaggtggg tgggcaggcc
     6421 gaccaccagg gtggccggct tccattcggc gaggagtttt tcgatggcgg caaagcgcgc
     6481 agcgttggct tcgtcaccaa tcaccgcgag gggatgggcc aggccggttt ccgtttcacc
     6541 caccgccacg ccgatgcggg ccagcccgaa gtcaaagccg aggacggtac tcacgcgtgg
     6601 ccggcgacgt ccgagagctt ggcgaaatcc acgccgagca gctgcatggc ggcggcgagg
     6661 cgctcttccg gcggcaggtc gaagacgatg gtcagatcgg ctggcacggt gagccaggcg
     6721 ttctgggtga gttcgtgttc gagctgtccg gcggaccagc cggcgtagcc gagggcgacc
     6781 agcacctcgg cgggctcgcc gtcgagggcg agggcctgga gcacgtcgcg ggagctggtg
     6841 aggccgatgt cgtccgtgac cttgaggctg gaatgccact ggccggccgg gcgatgcagc
     6901 acgaagcccc ggtcggactg caccgggccg ccgaaataca ccggcaggtc ggcaaaggca
     6961 ggctgctcga aggggatgtc gatgcgctcc atcagcgtgg ccaggcgcat gtccgtgggc
     7021 cggttgacga tcacccccat ggcgccgttc tcgttgtgtt cggcgatgta ggtgagggtc
     7081 cgcgcaaagt tcggatcggc catggcgggc atggcgatca ggaaatggtc ggtgagattg
     7141 atggtcggtt tcgtcacact gtctatttta agccgagaac ggcggaattg ttcctgcacg
     7201 ctgcggaaag ccgtggggac ggttgcgccg tcggggtttg ccgcgtgccg cgcggctcgt
     7261 taggatgccg gctccgtctg ccgggagtgt ccgatgactg ccgccctcgt gtggttccgc
     7321 cgcgatctgc gcgatgtgga ccacgccgcc ctgtaccacg ccctgcgccg ccacgatcgc
     7381 gtgcattgcg ccttcgtctt tgacaccgag atcctcgacg cgctgccggg ccgaatggac
     7441 cggcgcgtgg aattcatcca cggcagcgtg gtggcgctgg acgcggccct gcgggcgcgg
     7501 gggggcggac tgatcgtgcg ccacgggcgc gcccgcgacg tgatcccggc gcttgcagcg
     7561 tccctgggcg ttgcagaggt actgagcaac cgggactacg agccggcagc catcgcccgg
     7621 gacgacgagg tggcggcgcg tctgcggggc atgggcatcg gctttgcgga cttcaaggat
     7681 caggtgatct tcgagcgcgg cgaggtgctg acccagggcg gggcgccgtt ctcggtgttc
     7741 acgccttaca agcgggcctg gctgaaggcg ctgacgccct tttacgtcaa ggcctatccg
     7801 gtggaggcgt atgcgggcca cctggcgccg ccgccggacg ggaccaggat gccgaccctg
     7861 gccgagctgg gctttgcgac caccaacctg gcggaactgg gcgtgacgcc gggccgcgcg
     7921 ggcggacgcc ggctgctggc ggatttcctt acgcgcatcg ggcgttacaa gtcggcgcgg
     7981 gattttccgg cgcgcaaggg cgtgtcctac ctgtcggtgc atctgcgctt cggcaccgtg
     8041 tcgatccgcg agctggctgc cgccgcgctg gcggtggggg gcgagggcgc cgagacctgg
     8101 ctgtcggagc tgatctggcg cgatttctac cagcagatcc tgtggcatca cccgcgcgtc
     8161 gtggccgcca gcttccgtcc ggagtacgac cgggtggtgt gggacgacgc gccggacctg
     8221 ttcgccgcct ggtgcgaggc ccgcaccggc tatcccctcg tcgatgcggc gatgtgccag
     8281 ctcctgcaca gcggctacat gcacaaccgc ctgcgcatgg tggtggcgtc tttcctgacc
     8341 aaggacctgg gcatcgactg gcgcctgggc gaggccttct ttgccgagca cctgattgat
     8401 ttcgacctgg ccgccaacaa cggcggctgg caatgggcgg cgtccacggg ctgcgacgcg
     8461 caaccctatt tccgcatctt caatccggtc acccagtccg agaagtttga cccggatggt
     8521 gccttcatcc gccgctacct gccagcgttg gcgcgggtgc cggcgcgcta cctgcacgcc
     8581 ccgtggacga tgccgcccgt ggaccagaag ctcgcgggct gcgtgatcgg gcaggactat
     8641 cctgcgccgg tggtggatca cgcccgcgcg cgggcgcgca cgctggcgcg ctttgccgtc
     8701 gtgaagggtg cggacgggga cgtcgccgag gcgtgagatc tcagtccgcc ggcagcaggt
     8761 gggcgaagtc cgccgcggcc aggttggggg cgaattcgac gatgcggtat tccgccaggc
     8821 cgcgctggct gaaagggtct tcggccagca gggcggtgag cgcgtcgcgg ctcacgttgc
     8881 gggcgaggat cacgccaccg ctgcggggca cctggggccc ggagagcagg aaggtgccgg
     8941 cggcatagtg gcgggccagg aaggcgcggt gatcggggac ggcggcgtcc acctcggaga
     9001 gggggcgcag ataggtgacg atgacgacga acatgggggc tccgcagaat gaaaacggcc
     9061 cggcatgggg ccgggccgcc atgatgcccg gatttccgcc gggcgtcgtg ccgatcagcc
     9121 gtcgcagcgg gtgcgtttgc cgacggtggt gcaggcgcgg atcttcttgc ggtccatgtc
     9181 gtagaaggtg ctcgtccagt tggtggtgcc ggggatgcgg tcgaagacca cgaagccaaa
     9241 gtcggtaatc ctggccttgg ccgggtcgtt ggcattcacg atggggctgg tgctgaaggc
     9301 gtccggcagg gactgggcca ggctgccgaa ccacagggcc gccttggcgc ccagccaggg
     9361 ccacacgccc gaagtgatgg ccgtgccgaa gttggccggc gcgaagggtg cgcctgtggc
     9421 actgtcgaac atgaagcccg cttccagggt gtcgccggag acgccgatca ggaaggaggt
     9481 gggcaggttg ctcgccgccg gatggctgat catctgccag ccgtggatgt ggccggcatg
     9541 gagcatctgg gtgttggcgg gcaggctgcc gttgccgccg gcgtaggtct cgacgccgtt
     9601 cacgccgctg gcggcgatgg ccttctggta ctgccaggtg cgcggggtgg ccgcgccggt
     9661 gccgccggtg taggcgaaca ggggcttgtg ggtgacgatc caggtgacct tggtggggtc
     9721 cttggaggcg ctcaggttgg ccagggcgtt gagctggcgg gcggtgcgga tgtggtcgcc
     9781 gtgggtccag ccctggttct tgccgacgcc cggatcgtcc tgcaggccgc tgttgtcgag
     9841 cagcacgatc tgcagatcct tgagggtgat ggccaccggg tcgatgtagt cgctgcaggt
     9901 gctggggtcg taggtgggcg agtacttggg ctccttggcc gcgctggtgg cggccgggaa
     9961 ggggcgcgga tccaggaagt ggaaccagcc ggtggcggcg cgaaaacagg attcgtggtt
    10021 gccgcgggtg atggcccacg gcgcggcact gaggagggcg ccggtgggtt tgaagaagtc
    10081 ggcttcccag gcggcccagc cgtaggtgac ggtgggcatc aggcccttct gctgcaggaa
    10141 gtcctcgttc ttgccggtgc cggccacgcc ggcgggggtc tcgacgccga cgggcgtgcc
    10201 ttcccggtag tggatgtcgc cgttgtggat gacgagatcc gggttgaagc tggccgcggc
    10261 ggcggcgatg cgcttgtagg gccaggcgcg gtcgtcggtg cagtcctgca gcgggtcgcc
    10321 gccggcgccg ttggtgtagg cggtggggcc cttgatgcga cagccggtgt cgccgatcac
    10381 cacgatgcgg ttgagggcgg tgctgctgtt gaggagcttg agggcctggc cattgacgat
    10441 ggcggacctg gcggtgtcgg ggatgcggaa ttcgcaggtg agcacggcga agtcggcctt
    10501 gacggggacg ctcttgctgc cggtggagta ctggccgttg ctgtccacct gggtgatggc
    10561 cggcgcacgc acggccatgg gctgggcgac gccatccacg gtgatggacg ggcaggtggc
    10621 ggcggtggtg atggcgcggg cgtagagggc gccggcgctg accgggctgc cgttggagct
    10681 gtcgctgacg gtgtaggtgg tggcgtcggc gaccttgccc ttgaccggat cggcattgag
    10741 ggcgacggcg ctgctgctgc cggtgatgct caccttgctg aagggcgtgg tgtcgtcggc
    10801 acccacctgg gtccagacgt acagcacctt gctgtccacg gccttggcgg tgagggtctt
    10861 gcccatgccg cccttcaggt cgctgccggc ggtcttgacg gtggcggcga tggtctggat
    10921 ggcattgttg ccctgggcga ggagctggct cctggtgagg ctgtcggttt cggtgttgta
    10981 gtcgcccatc agcttggcgc catccacccc gaggcggccg gcgacggtgc tgcgggcgtc
    11041 ggccaggctg gcgccgccgt ccatctcggc gacgaccagg gtggtgaggg cgctcagcac
    11101 ggcggtgttg ccggcggggg cgcggaacac gagcttgtcg gtgacagccg aagcggtgcc
    11161 ggtgtcggga tcgagccggg aggcgtcggt gccgatctcg gcgaccaccg gcaccttggc
    11221 cacgggcaac gtgaagcggc cctgggcgtc gctgcgggtg gagaactcgt tggcgtcgca
    11281 gacggcgttg ttgttctcgt ccagacagac cttggcgttc tgaaagtagc tgccgactac
    11341 cacgccggtg ctggtgccga cgtccgatcc accgccgccg cagcccgtca gtccgctgcc
    11401 gattccgatc gtcgccagag cggcgaccag ggatttgacc tgccaggagc cttgcatgcc
    11461 cgtttccttc gcatctgaaa gcgcagaagg gtagggaatg tgtgtaacag cgtcgtgacg
    11521 gggcgggctc agcggcgggc cgtggcgatg aggacggtgc tttccagcgt gaagccggcg
    11581 ccgtcgcgat ggggcgcgag atcgtcggcg acggcggcgc gcagggtcgc ctgggtggcg
    11641 tcggggaggc gcagcagggc ttcggcggcg ccgcccgcca gatcgaggaa ggcctgccag
    11701 taggcgtcgg cggagtcgaa gcggcaggcc aggcggtggg gcgtcacggc gatggcgcgg
    11761 aagccggcgt cgtccagcag gtcgtgcagg acggctgcgt cgccgaggcg gaacaccgaa
    11821 ggccgcggca ccttgggcgc gggcagatgg cgggcgatgc agtcgagggc atgggcgatg
    11881 agggggacgt cgttgcgcgg accccacacg gacacggcga ggcgtccgcc ggggcgcagc
    11941 acgcggtgca gttcggccac tgcccgttcc gggtggggca ggatgaagag gccgaggccg
    12001 accagggctg cgtcgaagcg ggcgtcggca aagctcaggt gctcggcgtc gcaggcggcg
    12061 gcgaggaggt ccggcacgcc ttcgtcggcg gcgcggcgca ggccttcggc gagcatgcct
    12121 tcggcgatgt cgctggccac gacgcaaccg ccgggcagca cctggcgggc cgcggcgcgg
    12181 gcgagcaggc ccggcccggc ggccagatcg aggacgctct cgccgggcgc gagggcggcg
    12241 gcggcgagca gggcgtcctg caggtcggcg cgcagggggg cgccgtcggc gtagcgggcg
    12301 gcgatgcggt tgaagccgct gcgttccagg gccttgaagc ggcgggcgtc gaaaccgggc
    12361 ggatgggaca tgggcgggcg tggggtgcgt gggcgcggat caggggatca ggccggcgaa
    12421 gcgcaggatc gggtccaggt aggcgtccca gcgggtgaag ctgtggtcgc caccgtccag
    12481 cacggtctgg cggcagccgg catagcgggc gacggcgtgg cggtagtcga gcacctcatc
    12541 gccggtctcg gcgagcagcc agaagcgctc cgggtggctc agggcgggca cgtcgagggc
    12601 gcgcagctcg tcgatgtggg ctggcgtgaa gtcgaaggtc tcgcccgtgt agaggtgggt
    12661 ctgggggccc aggtaggcgc tcagggagag gggcgcgagg actgccggat tgaccaccac
    12721 ggcacgcagg ccgtgctttt ccgccaggta ggtggcgtag tagccgccga gggaactgcc
    12781 caccagggtg ggtgggcgcg ggctggcggc gatggccgct tcggccaggg ccatggtggc
    12841 gcgggccgac accggaagct gttcgcacca gaggcggtcg ccgaggccga gttcggccat
    12901 gcgcgccgcg aggctgcggg ctttccagga ttgagggccg gaacgaaagc cgtggaggta
    12961 gaggatcatt tgcgggcgta gcggtcgtcg ttgcggagta tgccggggag gccgctttcc
    13021 agccagtggc gggcgtcgct gtcgtcgagg aggtgggcgc gccagaaggc ggtgctggcg
    13081 gccaggagcg cattctggaa gggtggcggg gcgcggcgca ggcggaacat gccttgttcg
    13141 gcggggctgg actggccggc gaaatccagg tgggtgccgc cggcaaagac cagcaggtat
    13201 ttgtggcccg gcggcatgtg gcggaagggc agctcccggt tggcggcggt gatgtcggac
    13261 aggcccatgc cgtcgtcgcg gctgccggtg aggctgaaaa acgggcgctc gagggcgccg
    13321 aagcgctcgg tcagatcgtg gccctcgccc cgcgccgagg ggctgagggc gaggatcgcc
    13381 cgcacgcggg gatcgagggg gacgacacgc tgggtcgggc ggcgttcgcc ggccaggagc
    13441 tgcacggtga cggcgccgaa agagtggccg gccaggccga tgggcgcgcg gacgtctggc
    13501 agggcggggt cgtggccgag gcggtcgagg atgtggcgca ggtcgtcggc ccgctcggcg
    13561 aggcgttcgg gattcatcgc cgccttgaag gcgcggcgca gggcgagggg cgagccgtgc
    13621 tgcaggaggc cgtcgtcgct gccgggatgc tgcaccgcga gaccggcgaa tccccacgaa
    13681 gcccaatggg cgagccagtc agcgccacct tcgcgggagc cgccgaggcc gtgggagaag
    13741 aggatcagac cagccggcgc aacgccctgc ggcaggtagc gcttgagcgg caggctgcgt
    13801 gcgcggtgtt cgtcgtgcag cgtgtcgagc tgcgtgacga ccgggtgggg gcccgggcgg
    13861 ccgtccgcgg gggcgaaggg cgcgtccggg tcgggaggca ggctcagggg cgggccctca
    13921 gctgctggtc cattccacca gaccgaaatg ccaggtcgcc agcaccacca gcccgaagaa
    13981 gatgcggtac caggcgaagg gcacgaagtc gtggtgggag atgtagcgca gcagccagcg
    14041 cacacacagg aaggccgaga cgaaggcggc gatgaagccg acgacccaca tgccgatgtc
    14101 gtcggcgttg agcagggcgc gctccttgta caggttgtac agggtggcga tgaacagggt
    14161 ggggatggcc aggaagaagg agaactcggt ggccgccttg cgcgacaggc cgaagagcag
    14221 gccgccgatg atggtggcgc cggagcgcga ggtgccggga atcagcgcga aggcctgggc
    14281 gatgccgagc ttgagggcgt cgagcgggcg catctcgtcc acggagtgga tgcggatgcg
    14341 atggtcgcgg cgctcggccc acaggatgac gaaggcgccc acgatgaagg cgagggccac
    14401 cggcaccggt ttgaagaggt gggcttcgat gaccttcttg aaggccagcc cgagcacggc
    14461 caggggcagg aatgcgatgg ccacgttgag caccaggcgg ttggcttccc gctggcggcc
    14521 gaggccggcg aaggtgcggg cgaggcgctc gcggaattcc cacaccacgg cgaggatggc
    14581 accggactgg atgacgatct cgaagagctt gccgcggtcg tcgttgaagt tgaggaggtc
    14641 gccggcaagg atcaggtggc cggtgctgga gatgggcaga aactcggtga gcccttcgac
    14701 gacgcccagg ataagggcct tgacgagcag gatgatgtcc atggacgggt tgactgtgca
    14761 gtgcaaaaag agcgcgcatt atagccttgc tccatgacgc cttagacggg ccggcgacgc
    14821 tcggacacga acttcccgca cgccgccgtg acggggtgct cagttcaacg gcataccgct
    14881 tcaggtcggg gccgggcggc cgataccact ctggatgcga tccattggcc ccgtgcgatg
    14941 ttgaagaaga tccccgtaga gcggctcaga gccggcatgt tcatccacga gctgtgcggc
    15001 gactggatgt cccacccatt ctggcgtgcg cgtttcaggc tgaatggcgg tgacgacctg
    15061 cagcgcatcg tcgagtccgg catccgccat gtctatatcg acacggacaa gggtctcgac
    15121 gacgcggagg cggtggatgc cggtgccgtg cgcgaggccg tcgaagccca gatggtggcg
    15181 gctctggcga cgccggagga gacgcccctg cgggtgtcgg tggcggagga gatggagcgg
    15241 gcgcggcgtg tgcacgaaca ggcccaccgg gtggtgcgag ggatgatgca ggacgtgcgc
    15301 ctcgggcggg ccgtgtccct ggaggacgcg gaaccggtgg tggaggccat caccggctcg
    15361 gtgctgcgca acagcggcgc gctgatcggg ctgatcggca tcaagaacaa ggacgactac
    15421 acctttctgc attcggtaag cgtctgcacg ctcatgatcg ccttcggccg ttcgctcggg
    15481 ctggccggcg aggatctgcg ccaggccggg atcggcgggc tgctccatga cgtgggcaag
    15541 atgaaggtcc ccgatgcggt cctgaacaag ccggggcggc tcaccgacgc ggagttcgcc
    15601 ctcatcaaga tgcacccggg cgacggccac gcggtccttc tggagacgcc aggcatcggc
    15661 ccggtgcctc tggacatcac ccggcatcac cacgagcgcc tggacggcag tggctacccg
    15721 gacggccagc agggcgaggc catcagcagc atggcgcgca tggcggccat cgtggatgtc
    15781 tacgacgcga tcaccgccga ccgctgctac caccagggtc tgccggcggc ggaagccctg
    15841 cgcaagatgt gggagtggag cgcggcccac ttcgatcaga agctgctgca ggccttcatg
    15901 cgctgcgtgg ggatttatcc ggtgggcagc ctggtacgcc tggaatcggg ccgcctcggg
    15961 gtggtgatcg accagaacga gggtgcgctg ctcacgccgc gggtgcgggt gttcttcagt
    16021 acccgctcaa acgcccacat cccgcccgag atcgtggatc tcggtcgcaa gccgggccac
    16081 ggtggaggcg accggatcgt ctccgccgag gcgccggaca aatggggcgt ggatccggcg
    16141 cgcttcctgc tgcgggaggg gtgatccggc gtcagtgccg caggcgcgcg tcgaggctgt
    16201 cgatcagttc ggcccagtcg gcgtcgtcgg ccagctcgcg ggccaggaag tcggcctggg
    16261 gcttcgtcca gaagggcgcg tcggccagac gcatgtcggg cggcaggctg tggctgtcga
    16321 ggaaggcttc gatggcgccg ctgtcggcgg gcaggcccag ctgttcgaac aggtggccga
    16381 gggtatgagt cggggcttgc atggcgttct ccttttcgtg ggggcgggca tgacaggatt
    16441 tttgcaccca gggattgggc aacgggtcgg cggaatggtt tccgggatat tccgccggag
    16501 cctgacattg ccggcgcagc tctgggacaa tggggctccc catcccccag aaggagtccg
    16561 ccatgtcgtc accgctcaaa atcgtcaccc tgctcggcag cctgcgccag ggctcctaca
    16621 acgcggccat cgcccgggcc ctgccgaccg tggcggggga ggacgtggtg gtcgaggcgc
    16681 tgccctcgat ccgcgacatc cccgtgtacg acgccgacct gcaggcggag ggctttccgc
    16741 ctgccgtcat tgccctggcg gacgcgatcc gcgcggccga cggggtggtg atcgtcacgc
    16801 ccgaatacaa ctattccgtg cccggcgccc tcaagaacgc catcgactgg ctgtcccgcg
    16861 tgccggagca gcccttcgcc ggcaagccgg tggcgatcca gactgcgtcc atgggcatcc
    16921 tgggcggtgc ccgctgccag tatcacctgc gccaggtgct ggtcttcctg gactgccacg
    16981 tcctcaacaa gcctgaggtc atgatcggtg cggtacagga caaggtgaac gccgcgggcg
    17041 aacttgcgga caccggcacc ctcaagcgcc tggacgaaca actgttggcg ttcaccaagt
    17101 ttatccgccg cctgggatga gtctcgcgga ctgggtggcc cattacggct atttcgccgt
    17161 ggcggtgggc tgcctgctgg aaggcgaaac cgtgctgctg ctggccggtg tggcggtgca
    17221 tcggggtctt ctcgatctgc ccgccgtggc ggcggtggcg tttgtggcgg gattcgccgg
    17281 tgatcaggcc tggttttttg tcgggcggcg cttcggagac cggctgatcc ggcgattcgc
    17341 agcggtgcag cgggccgttc cccgcatcca ggacctgatc ctgcggcatc agaacctcct
    17401 cgttctgggc atccgcttca tgatcggcct gcgcatcgcc gggcccgtgc tgatgggctg
    17461 gagtcgggtg caggccttgc gcttcgtgct tctcaacctt ctcggggccg cgctctgggt
    17521 ggcggtgatc ggcggcggcg gctattactt cagcgccctg atggagcggc tgctgccgga
    17581 cctgcggcgg gccgaagagg gacttctggc cgccaccgcg gcggctggtc tggtcgcaca
    17641 tggggtacgg ctgtggcggg gacgtgccgc gcggggcaga aataaagtat attagtatgt
    17701 actgattaat ttaggattag gatgttccga tgcgtcacat gttcatcgct gccctcgccc
    17761 tgagtgcggg catcgcccag gccgccgacc tggacgccct caaatccgaa acccgccaga
    17821 aggccctgcc cgtgctgccc aaggtggcgg gcatgatgaa ggagaccgtc gtcgccgaag
    17881 gtacggtcgc cgccatcccc gtgtgcaagg aaaaggcccc gcaactcctc cgggcgctct
    17941 cggccgaaac cggctgggac atccgccggg tcagcctgaa gacgcgcaat ccccagcgcg
    18001 gcacgccaga cgtatgggag gcgcgccagc tcgccgactt caacatccgc gctgccaatg
    18061 gcgagaaggt cgagaccctc gaaaccggcg agatcgtcag cgagcacggc aagccggttt
    18121 accgctacat ccgggccatt ccggcgggcg acgtgtgtct ggcctgccac ggggccgcgg
    18181 acaccctgga cgcgcccttg cgggagcaac tcgcccgcca ttatcccgat gaccgggcga
    18241 cgggctatac caagggccag atccggggag ccctgacggt caagcggccg ctctgagcgt
    18301 ctgttacagc cttacaggac gttaccgttt gcctgttctc ccgggggaca ggcgccgcgt
    18361 tgagggaagg gcgagccgcc gcgatgcggt gaccgcccta aacccccgaa ccgacaggag
    18421 aacgtccatg aacaagtccc tcttcgccct gagccttgcg ctggccctcg gtgccgccca
    18481 cgctgccgac gtcaagcccg ccgacccgcg ggcaacgccg gccgatgccg tgaaggccgg
    18541 ggccggtgcc gccgcagccg gacagacagc gatggagcag cacgtccgca cccatgctgc
    18601 cgggaaggcg gaggctgtga agcgcgcggc ggtaccgcgc gaagcgcagg cgtccgccgc
    18661 agtccgggtg aaggacgccg ttgccccgca gggcgcgcgg atggacgggg ccaaggcggc
    18721 ggtgaaggcc gaggccaagc ccgctgacca ggccgcggcg ctggcgggtg cagtcgtgaa
    18781 gtaagggtcg ggtgcaggcg cggcgacacg tgcctgcggc tgaacgcgcg gggcggagcc
    18841 tgtgagggcc tccgccccgt gtgcgtggtg cgatggcggg aaacggttgc ggccgtgtgg
    18901 ggttctctgg caaggtgcgc tgctgtcgcg catcgcgatg gcatgtacgg gccgccgacg
    18961 ccgccgggat cacgggcgcg ccggtccggc gccgtccgct gcggtttcca cccgtcctgt
    19021 tccgcgaggt ttcccgtgac gctccgcacc gccagtctgc tgtttgccct gctgtcttcc
    19081 actgcccacg cctgcggtcc ctatgaggac gactgggccg ggcgcgacaa ggcggcccat
    19141 ttcggggtgt ctgcagccct cggcgtggcg acgtcccaga tcagcagcga tccgtggacg
    19201 gcctttgccc tggcgctggc ccccggtgtg gcaaaggagg tctacgactc gcagcgcgcg
    19261 tgcaaccgct tctcgtggaa ggatctgagc tgggacgccg ccggcgccct ggtgggcgtg
    19321 aagaccggcc actggctgtt gtcgccgggt ggcgtggcgt accgcaggtc gttctgagcc
    19381 cgggaccggc cggggacttc agaccggccg gtcgcagggc tgcgcttcgg cggggatcgg
    19441 cgccgtccat cccttcaccc gcagcagcgt ctgctgcagt acgcgggcgt cgttggaggc
    19501 acggtgacgt tgcaggccca gctccgccag gatgtcggtc ttggtggcat gccagacctc
    19561 catgtcggtc tcgccaagca ggctgcggat gtcttcaagg cggaacagct ggggcagatc
    19621 ggcggcatcg aagaggcggc ccagccaggt gaagtcgtga taccaggcgt cgcagtagat
    19681 cgtctgtccg tgcaggcgga ggttgagggc gtgggccacg tcggcggggc tgcggccgtg
    19741 ggccacgagc gtgctgcggg cgacacggtg gatcgcctcg gcgctcgcgt cccagtgggt
    19801 ccaggcgtca aggggccgga tcagcgaaca ataggtgcac ccatccggca ggatcaggcc
    19861 gacctcgatg ggatagctgg atggtccgaa gccggacgct tcgatgtcga ggatgggggg
    19921 cggacggaag gcgggcgcgg tggctggcat ggatcgtttt ggcgggcgcg tcgaggcgcc
    19981 gggcgcggcg gtgcttgagt cgacgcaggg acgggcgcat gatcgccccc tcctgcgctt
    20041 cccgaattat ccccgctccg cgtccgaaat cctactggca tgccaatcga cccgtccgcg
    20101 accttccctg tccatcctcg cccctgggcg ggggccctgc tgcgacgcct ggggtggacc
    20161 ctcgatgtgg cgccattgcc tgcggcccat tgcctgatcg tcgtctatcc ccacacctcc
    20221 aactgggact tccccctggg cctgctggtg cgttttgccc tgggctggcc gatcggctgg
    20281 atcggcaagc acaccctgtt ccgctggccg gtgagcgggc tgctccgccg attggggggg
    20341 attcccgttg accggggaca gccgggcggt ttcgtggagg gcatggcggg ggcgttccgt
    20401 tcccgcgatc acctgctggt cgccatcgcg ccggagggca ctcgccgtcg caccgccggc
    20461 tggaaatccg gcttctatcg cattgcgcgg gctgcggacg tgccggtggg gctgggcttc
    20521 atcgactacc gacgcaagcg cctggggatt gccgcttacg tccgcctcac gggcaacgtg
    20581 gcggcggaca tggcgcgcat ccgcgaggtc tatgccggga tgtgcggcta ccggccggat
    20641 caggcgagcc cggtggtgct gccggagagc gagcgcctgt aggcctgccg cacgacgcgg
    20701 cctgtcatgg cggcggggta gaatgaccgg cacatccatc gccggcccgc cacgccatga
    20761 gtgaatccga cctgtcccgt ctgagcatcg accgcaacgc gccgcggccg tctcgccgac
    20821 gcgcccgggc ctggctgatc ggtggcgcac tggtggtggt ggccgcgctt gccgctgcga
    20881 tcgggcactt caatgcgccg gtgtcggtcg agaccgtcag cgtcggcgag gcgtggccct
    20941 atcagggcgt gaccgtcctc aacgccaccg gctacgtggt ggcccagcgc aaggcatcgg
    21001 tggcctccaa ggccaccggg cgactggaat ggctgggagt gcgcgagggc tcggtggtca
    21061 aggccggtga gctgatcgcc cgcctggaac agcgcgacgt ggtggcgcag gccgagcagg
    21121 cggcagccaa cgtgggggtg gcgcgggcca acgtggggca ggcgcgggcc gagctcaagg
    21181 atgccgagct ggccctcggc cgcgcccgtg atctgcgcgc ccgcaacttc gtcgcccagg
    21241 cccaggtgga tcaggccgag gcccgcgcga cgaaggcccg cgcggcgctc gggtcggccc
    21301 aggccgccga gacggctgcg gtggcggcgg agcgggcggc ccgcgtggcg gtggcccaga
    21361 ccgagatccg cgcgcccttc gacggcgtgg tcctcaccaa gaacgccaac gtgggcgacc
    21421 tgatcaccaa tttttccgcc gccgccgaca gcaagggcgc cgtggtgagc atggcggaca
    21481 tgggcacgct ggaggtggag gcggacgtct ccgaggccaa cctggggcgc atccgcgtgg
    21541 gccagccctg tgagatccag ctcgacgcct ttccggagca acgcttccgc ggcgaggtgt
    21601 cgcgccgggt gccgacggtg gaccgggcca aggcgagcgt gctgaccaag gtccgcttcc
    21661 tcgatccgga tccgcgcgtc ctgccagaga tgagcgccaa gatcgccttc ctggacaagg
    21721 cgctggcgga cgccgagcgc acgccacggg tggcggtgca cgaagatgcg ctgcggcgcg
    21781 aaggctcgga agtggcgctg ttccgcgtgg aggacgggcg ggcccaccgc gttgtcgtgc
    21841 agacgggcgc aacgatcggc gaactggtcg ccgtgcaggg cgtcaagtcc ggcgacaagg
    21901 tggtgctgcg tccgccggaa tccctgcgcg acggggccgc ggtgaaggcg gggggcaagt
    21961 gaccgacgcc cgcgggagcg ctgccggcgc gccgattgtg cgtctgcgcg atgtgcgcaa
    22021 ggcctatcgg cgcggcgccc aggtggtgcc ggtgctggca ggcatcggtt ttgacattgc
    22081 ggagggcgaa ttcctggccc tgatggggcc ctccggctcg ggcaagagca cgctgctcaa
    22141 cctcatcgca ggcatcgacc gggccgacag cggcgaactg atcgtcgccg gcgaggacat
    22201 cgcccgtctg gccgagcggg agctggccga ctggcgggcg cgccacattg gattcatctt
    22261 ccagttctac aacctgatgc cggtgctcga cgcgctcgag aatgtggagc tgcctctgct
    22321 gctcaccacg ctgtcggcgc gggagcggcg cgagcgggcg gccttcgcgc tgagcatggt
    22381 ggggctggcc gaccgtatgg accacacgcc gggcgagctt tcgggcgggc agcagcagcg
    22441 ggtggctatc gcccgcgcgc tggtgacgga cccgactctg atcgtggctg acgagcccac
    22501 cggcgatctg gaccgggagt ccgccaccga gatcctcggg ctgctgcagc gcctcaaccg
    22561 ggaggcgggg aagaccatcg tgatggtgac ccacgaccag cgcgcggcgg cggcggccca
    22621 cgcgatcatg cacctggaga agggcgagct gtcggggcgc gaggcggtgc ggacatgacg
    22681 cagccacggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagccgcagc
    22801 gcaggctgcg gctcttgcag atcgacccgt gccggccgcg ggggcgggca cgcgtggatc
    22861 aggtgctcat gccaccggcg atgtagcctg cgagctgctc gatggtcgcc tgctgctgct
    22921 ccatgaccga cttgaccgca tcaccgatgc tgatgatgcc ggccaggacg ccgtggtcca
    22981 ccaccggcag gtggcggatg tggttgttgg tcatgatgcc catgacttcc tcgacggtct
    23041 ggctcggcga gaccgtcagc acgttctggg tcatcagatc gccgaccttg gtgtctttgg
    23101 acgccaggcc gcgcagcacc accttgcggg cgtagtcgcg ctcggtgaag atgcccacca
    23161 gccgcgggcc ttcggtgacc agcacagcac tgatgttgcg ctcggccatc agcttgaggg
    23221 cttcgagcac cgtggtgtcg ggcgcgacac cgtgacaggt gccgcctttg ttgttcagaa
    23281 tctgctggac cgtggttgcc attcttttgg atcctcctcc cgattgttgg cttggtctga
    23341 ggggcagcat cgcgtccgga gccaccctgt gagcctgcat agcttggggc gaagcgcggc
    23401 ggagcgcaag agggcataca ggttttttgt gcaaaccggg atggcgccgg catgctgacg
    23461 gctttcctgc ggatgatcct gcgcaatgcc tggcgccacc gtttgcgcgc cggcctgacg
    23521 gtgctgggca tggtggtggc aattctcgcc tatggcctgc tcgggaccgt cgtggatgcc
    23581 tggtacgcgg gggcggaagg cgcctcggcc aaccggctga tcacgcgcag ttccatctcc
    23641 ctggtgtttc cgctgccgct cacctatcgg gaccgcattc gcggagtgga aggcgtgcgc
    23701 gatctgtcct gggccagctg gttcggcggc atctacaagg agccgaagaa cttcttcgcc
    23761 cagtttgcgg tggacggtga aagctacttc cgcatctatc ctgaattcgt cgtggacgaa
    23821 cgggccttcc gcgattttct gcgggatcgc cgtggctgcg tggtggggcg caagctggcc
    23881 gagacctacg gcttcaaggt cggcgacacc ctcacgctca agggcacgat ctatcccggc
    23941 aactgggatt tcgtcgtgcg ggcgatctac gacggcgcca ggaagggcac cgatcagtcc
    24001 cagatgctgt tccattggca gtatctggac gagaccatca agcagcgcta tccgggacgt
    24061 gccaacaacg tgggtgtgtt cgtcaccgaa gtggcgtcgc cggaccaggt ggcagccgtg
    24121 tcccgggctg tcgaccggga gttcgccaat tcccgcgccg agaccctcac cgagacggaa
    24181 aaggccttcc agctgagctt cgtggcgatg acggaggcca tcgtggtggc gatccgcatc
    24241 gtgtcctacc tggtcatctt catcatcatg gcagtgatgg ccaacaccat ggcgatgacc
    24301 gcccgcgaac gtacggcgga atatgccacc ctgaaggcac tgggcttcgc gccggccttc
    24361 gtcggactgc tgatcctcgg cgagagcctg atgctggcgc tcaccggcgg tgccatcggc
    24421 atcgctttga cttttcccgt ggcggacgcc tttgctgcga agatgggcac gctcttcccg
    24481 gtgttcaacg tctctaccga aaccgtggcg ctacagattg cctgcgcgct gacggtgggc
    24541 atggtggcgg cgctgctgcc catgctgcag gtgggccgcc tgcgcatcgt ggacggcctg
    24601 cgggcggtgg gctgaggcat gaagcggatt cccctggcct acagcctgcg caacctgtgg
    24661 gcgcggcgcc tgaccacggc cctgacggcc gggggcatgg ccctggtggt gttcgtcttc
    24721 gctgcggtgc tgatgctcga ccagggcctc aaggaggcgc tggtggacac gggccaggcg
    24781 gacaacgtgg tggtgatccg caaggggtct gaaaccgagg tgcagagcat catcgagcgc
    24841 aatcaggcgg cgctgctgga gactttgcct caggtggcgc tggcggcggg ggggctgcct
    24901 caggtctcca aggagaccgt tgtcctgatc acgctcccca agcatggcgg caagccggcc
    24961 aacgtgatca tccgcggcgt gggggggcag ggcgcggccc tgcggccgac cctggcgctg
    25021 gtggaagggc ggatgttccg gcccggctcg gccgagattg tcgtcggggc cagcatcgcc
    25081 cgccgcttcg acggcaccgg gctgggcagc acgctgcgtt tcggtgcgcg ggactggcgc
    25141 gtggtggggg tgttcgaggc ggacaagagc ggcttcgaat cggagatctg gggcgacgcg
    25201 gaccagctga tgcaggcctt ccggcgcccg gcctattcgt ccgtggtgct gcgtctggcc
    25261 gacccggcga gcttccccgc cgtacgcgcg ctgatcgacg acgatccgcg cctgagcctg
    25321 gaggccaaga ccgagcgggc gttctacgcc gatcagtcgg ccatgctgtc caacttcatc
    25381 agcatcctcg gcatgaccct gtcggtgatc ttctcggtcg gcgcaatgat cggtgcggcg
    25441 atcactatgt acgcggcggt cgcgacccgt actgccgaga tcggcaccct gcgggcactg
    25501 ggcttccggc gcggcagtgt actgctggcc tttctgacgg agagcctgtt gctggccctg
    25561 ctgggtgggg ctgcggggct ggcggcggcg tcgctgatgc agttcttcac catctcgacg
    25621 atgaactggc aatcgttttc agagctggcc ttcagcttcc gcctgacgcc ggccatcgtc
    25681 gtcaagacgc tgatctttgc gctggcgatg ggactcatcg gtggcttcat gccggcggcg
    25741 cgggcggcgc gcatgaacat cgtgtcggcc ctgcgcgatg catgagcgcc gcggggggcg
    25801 tctacctgca gggcgtggcg tgacctactt aacgacgtgg gccaaagttg tggggatttg
    25861 acagtcatcc acggaaaagc gtgattttgt ccgccatgcc agacgggggc acgtcctact
    25921 ctggacgcat gacaaccccg ttgcatggag cccgatcatg tatgtcgcac ttttctgtct
    25981 gattggcatc gtcgccctgg cggcgatcag cgcccaggcc tggcgcgacc gcgcgcgagt
    26041 ggcagcttcc gtcacgccgg actggcatga cacgctgcgt cgcctcgcga ccgaacagga
    26101 actgggtcat taccgctcgg cccgcgaaaa gacggtttca gtcctgcgcc gcctcgagag
    26161 ccgccgcttc gacccgcgca tggccgccca cgtacgggtg cgcatcgcct cgctgatggc
    26221 ccgcgatccg gtttataccg aagtggtgcg cgccatccgc aaggcctgcg cggcgcagac
    26281 gtcgatcagc gagatggccc tgtgcgtggg cctgcgccag ttcctgatcg aagacatccg
    26341 catcagcatg gaaatcgccg agatgctcgg ccaggtccgg cgtgccgaag agggtggcag
    26401 cccgacggtg atggcggaat tccatcccac cgcctgaaac cctttacccg gccacgacgc
    26461 ccgccgatcc gcgacagcgc gggggcgggc gttgtggcgt ttacagttcc agattgtcga
    26521 tgaggcgggt gctgcccagg cgggcggcgg cgaggacgac caggggctcg tcgccttcgg
    26581 gcacctgcag gtcgctgcga cgacggatgg cgatgtagtc gggctgccag ccgtgggcgg
    26641 cgagctcggc caccgcatcg gcctccatcc gggccagatc ccgttcgccg gcctgcaggc
    26701 gctcgcggat gcggctgagc tgggcaaaga ggcgcggcgc ctcggcccgc tcggcggcgc
    26761 tcaggtagcc gttgcgggac gacagggcga ggccgtcggc ggcgcggatg gtctcgccgg
    26821 ggacaatccg gatcggcagt gccagctcgc gggtcatgtt ggtgaggacc atgagctgct
    26881 ggtagtcctt cttgccgaac agggcgacct ggggctgtac gatgttgaag agcttgagca
    26941 ccacggtggc gacgccgcgg aagtggccgg gacgcacggc gccttcgagg atgctcacct
    27001 gggccggctc gggctctacg tggtagcgct ggggcgcggg gtacatctcg gtctcgtccg
    27061 gcgcgaagag gtgatccacg ccggcgtcgg tgaggcgcgc gcagtcgtcg gcgaaggtgc
    27121 gcgggtactt gtcgaagtct tcccggggtc cgaactgcag gcgattcacg aagatgctgg
    27181 cgacgaccac atcggcgtgc cggcgggctt cgcgcatcag ggcgatgtgc ccgtcgtgga
    27241 ggttgcccat ggtgggcacg aaggcgacgc ggccggcgcc ggccagggcg gcgcgcaggc
    27301 cggcgatggt aggatggatc tgcatggcgg gatcagtagc tgtgttcggg gccggggaaa
    27361 cggccctcac gcacatcggt gacgtaacgg gtgacggcgt cgtccaggct ggcggcgcct
    27421 tccatgaagt tcttggcgaa gcgcggccgg cggccgggga agacgccgat gaggtcgtgc
    27481 agcaccagca cctgaccgtg gcagtccacg ccggcgccga tgccgatggt ggccatggtg
    27541 gtgaggctgc gggtgagatc ggtggccagg gcggcgggga tcatctccag caccatcagg
    27601 gcggcgcccg cctgctccag ggcgaaggcg tcggccttga gcgtggcggc gcccgagtca
    27661 ctgcggccct gcacccggta tccgccgagc tggtgcacgg actggggcgt gaggccgatg
    27721 tgggcacaca ccgggatgcc ccgttcgaca aggaaacgca cggtctcggc catgacggcg
    27781 ccaccttcga gcttgaccat ctgggcgccg gcggccatca ggcgggcggc gttgcggata
    27841 gcctgttccg ggccctcggc atagctgccg aagggcatgt cggcgacgat gaagggccgg
    27901 ctgcagccgc gggcgacgca ttcggtgtga tagaccatgt gctccatggt caccggtagg
    27961 gtggtcttct ggccctgcag cacattgccc agggagtcac cgacgaggat cacgtccacg
    28021 ccgcagcgct ccagcaggga tgcgaagctg gcgtcgtagc ccgtgagcat gacgatccgg
    28081 cgcccttcgg cgcgcatctt gcccagttca aaaagggtga cgggtttggc ggtgtcctgg
    28141 aggtaggtca tggcggcttc tccttgcggg cggcagttaa ccccaaatcc tgtgcagcgc
    28201 acaaggggtt ccggcgaagg ggcgggtgag ggcggaagcg gggcaggcgg cggcggacgt
    28261 ctggcggcgg gctggtcagc ccgcgtagcc gaagaactcc cgatagctgc gcatggccag
    28321 caggcgggaa tacagcagtt cgaaatcctc gtccttgtcc accgggttga gggattcggc
    28381 attgacgatg aacaggggcg ccgcatcgta atggtggaag aagcgcgcgt aatgctcggc
    28441 gacgcgctgc aggtaggatt cgctgatctt gcgctcggcc tcgacaccgc gcttgcgcac
    28501 gcgttcgatg aggatgtcgg gctgggcctg caggtagatc accagatccg gctggcgcac
    28561 ggtgggcttg aggcgctcga agagctggcg gtagagctgg agctcgtcca cgtccaggtt
    28621 gagttcggcg aagaggggat ccttgtccag caggaagtcg gagaccgcgc ggcggaaggg
    28681 gtcgtggtcg cgggtgaaca gggcgagctg ttcgctgcgc tggaacagga aggcgatctg
    28741 ggtggcgagg ccccagcgct cggcgtcctg gtaaaagcgc ccgaggaagg gattggtggc
    28801 cggattctcc agcagcagat cggcgtgaag acgctccgcg aggcgcttgg cgagggtggt
    28861 cttgccggcg ccgatggggc cttccacgac gatgtagcgg gctttttcca gcatggtgcg
    28921 ggcgggccgt caggtccgga agatgaagta gacggcgcct aggatacaca gggccgccca
    28981 cagatagtca agcttgaggg gctgctgcat gtagaacacg gcgaagggta cgaagaccgt
    29041 gagggtgatc gcctcctgga ggatcttgag ctggggcaga tcgagctgcc gggcgccaat
    29101 gcggttggcc ggcacctgta aaaggtactc gaagagggcg atgccccagc tcaccagggc
    29161 tgcgatgtac cacggggcgg aggcgaggtt cttcaggtgg ccgtaccacg cgaaggtcat
    29221 gaacacgttg gaggccgcca gcagcagggc ggtctgcagc cagatcggca tcacagggct
    29281 tcggcgcggg cgatggcctg gccggccacc gcggggaggg cctcgcgggc ggggccgcgg
    29341 ccgggaatcc acacgtcggg tgcgatctcc agcaggggca ggagcacgaa ggcgcgctcg
    29401 tgcatgcgcg ggtggggcag gatcagttcg gccgagctgc atgcctggtc gccgtggagc
    29461 agcaggtcca ggtccagggt gcgcggggca ttgggcacac tgcgcacgcg gccgtggcgg
    29521 gcctccacgg cgaggaggcc gcgcagcagg tcgagggccg gcagggacgt gtccacggcc
    29581 accacggcgt tgaagaaatc cggctggttg acgagaccca ccggcgcact gcggtagagg
    29641 gacgaacgga tcacgaccga ggtgccgggc agggcgccca ggtcggcaac cgccgcacgc
    29701 agggtcggga cggggtcgcc gagattggcc cccagcgcca cgtaggcgcg ggtgccggcg
    29761 gacacggcag aactcattcg ctgatggcgg cggcgggctt cttgcggcgg cggcgcttgc
    29821 ggggggcgct gccggccttt tcgggcagca gcatggcttc ccgcgcgtcc gggtgggcgt
    29881 cggcgaaggc gtcccaccag tcgggcagtt ccatggggat ctcgccggcc tgggcgcgca
    29941 ggcgcaggaa gtcccagccg gcccggtagc gggcctgctc cagcaagcgg taaggcgcct
    30001 tgccggcgcg cttgtcgaag cgcggctgca gcgcccagat gtccttgatg tcgccgacga
    30061 tgcggcgggt gatggcgagc ttgccggact gtttgtcgag caccgcgtcc atggcctcga
    30121 agagggccgg ctgggcgtgc tgaccgtccg ccttgcgggc ctcccagtcg gccagcacct
    30181 cgtgccacag cagggtggcg aagaggaagc cgggggacac gggcttgccc tgacgcacgc
    30241 gctcgtcggt gttctcgagg gacaaccaga cgaagcgttc gcccaggggt tgctcgagga
    30301 tgacgtcgag gaggggcagc aggccgtggt gcaggccttc ctcgcgcagc tgcttgaggc
    30361 acttgacggc gtggcccgac agcaggagct tcagcatctc gtcgaagagg cgtgccggcg
    30421 ggacgttttc gaggagatcg gccatctcgc ggatggggcc gcgggccgcc gggtcgatgc
    30481 ccagacccag cttggcgccc aggcgtgcgg cgcggagcat gcgcaccggg tcttcgcggt
    30541 agcgcacgcg cggatcgccg atcatgcgca gggtgcgctg ctggaggtcg gcgacgccgt
    30601 ggtggtaatc caccacggtt tcgtcggagg ggtcgtagta cagggcgttg acggtgaagt
    30661 cgcggcgcgt ggcgtcctcg gccatggagc cgaagacgtt gtcgcgcagc acgcggccgt
    30721 gttcgtcggt ctcggtggat tccgcgtcct gcatggcgcg gaaggtggag acctcgatgg
    30781 tctccgggcc gctcatgacg tgcacgatct tgaagcggcg gccaatgatg cgggagcggc
    30841 ggaagagggc gcgaacctct tcgggcgtgg cgctggtggc cacgtcaaaa tccttgggcg
    30901 tctggccgac gatgaggtcg cgcacggcac cgcccaccac gtaggccttg tgaccggcgt
    30961 cctggagcac ggtacagacc ttgatggcgg cgggagagag ctggtcgcgg cggatgccgt
    31021 gccgggcagc ggggatcacg gccggttcgg gcagggcgtt ggcgggtgcg gcgcggcgaa
    31081 acacgcggcg cagcagtttg cggatcattg gaacttcgtt acggttttgg gtcaagctta
    31141 cgatgatacc ccatcggacc gtagggtttg tgccacgggt gcccgaacga agcgtcaaaa
    31201 ggtaagcttg gcgaggattt tccgtgtccc ggggctcgcg atgcttaatt tttcagccaa
    31261 tctgtccttt ctgttcctgg aacgcccttt tctggaacgc ttcgaggccg ccgcggcctg
    31321 cggattccgc ggggtcgaat ttcatttccc atatgcgttt ccggtggatg aggtggtcga
    31381 cgccgcgcgc cgcgcggggg tggaggtggt gctcttcaac gtgcccgcgg gagactgggc
    31441 gggcggtgaa cggggcttgg gctgtcttcc ggaacggaga acggagtttc gcgatggggt
    31501 cggcctggcc gcgacgtatg ccgcggccct ggcgtgcccg cgggtaaacg tgctcgcggg
    31561 cctgcggccg agcggcgtgg cggacgcggt gttgcgcgcc accttgatcg acaacctccg
    31621 ctacgcggcg gaccgcctct cggaaagcgg ccgcacggtg ctgctggagc ccctcaatac
    31681 gcgtgacacg ccgggctttt tcgtcagccg cagccacgat gggctggcgc tgctggccga
    31741 ggccgggcgc ccgaacctgc gtctgcagta cgacgtctac cacgcccagg tgatggaagg
    31801 cgatctggcc cgcaccctcg gtgagcatct ggcgcagatc ggtcatatcc agctggctga
    31861 caatcccggg cgtcaccagc ccggcaccgg cgagatccgc ttccctttcc tgttcggtca
    31921 cctggaggcg cttgggtacg ccggctggat cggctgcgag tacgtgccga ccgggcgcag
    31981 tgaggattcc ttcgcctggc gggaagactg ggtcgacagc ggctgaggcg ccggattgcg
    32041 cgcgggcgga gggcggcgga tcgggagtgg cgcccacgct ggcgaagcgt gtcgcgacgg
    32101 cgggcggcgt ctgcatgcac cggcgaggct ccgatggcgg gcgtgggatt gcaaatcggc
    32161 gccaggcgct tacacttcag gataatttgt cagacaagta gacgaatgtc cgttgtgccc
    32221 ctgacccctg tgctccgccc gggccgtctc gccgaagagg ttggtgcaga cctcgagcgc
    32281 cgcatccgtc gcggtgattt tccgccgggc ggtcgcctgc cctccgagtc ccagctcgcc
    32341 gcccagtttg gagtgagccg gacggtggtg cgggaggcca tcgcgcgact gcgggcagag
    32401 gggctggtgg acagccgtca gggcgctggt gcgttcgtga tgcagcgacc gggcgcggcg
    32461 agctttcgct tgcgtgacgt gtcggcggag ccccgcagcg acgccgaggt cttcgaactg
    32521 cgtctcgtcg tggaggcggc agtcgccgaa cgggccgcgc ggcggcacac ggcggctgat
    32581 ctggcggcga tcgccggggc cctggcacgg atggatgcag ccttgcgcgc cggagagggc
    32641 ggtgccgagg cggacgatgc cttccaccgc gccattgccg ccgcctgcgg caatgcgctg
    32701 ttgcagcggc tggtggaatt cgtcagccag gatctgtcgg catcccgtcg tccgacctgg
    32761 agtcgcgacg ggcttatcgc cgggcgggcg ctgcgggcgc aggacgaaca ccgggccctt
    32821 ttcgaggcca ttgcagcggg agacggggcg gctgcccggc gtgcggcgga gcagcatctg
    32881 atcggaaccg cggcgcggct gggggtgtcc tggtcccttg atcccgccag cccgtcaaag
    32941 gagccgacat gactgcagac gcccacacgt ttccggattt cagtgaacgg gagcgggatt
    33001 tctcccgggt ggacaagcac cgccttgtca ccgccctgtc ccgcgcgctg ccagcgcacg
    33061 ggctgctcca cgagccggag gatctgcgtc cttacgaatg tgacggcatg tcggccctgc
    33121 gccagctgcc actggtggtg gccctgccgg acacggaggc acaggtggtg gagatcctgc
    33181 gcatctgcgc ggcgcagcag gtgccggtag tggcccgtgg cgccggcacc ggcctgtccg
    33241 gcggcgcgca gccccatcgt ttcggggtgg tgctgtccct ggcgcgcttc aagaagatcc
    33301 tgcacatcga cgcgcacgcc cggcgggcgg tggtgcaacc gggcgtgcgc aacctggcca
    33361 tctcggaggc ggccgcgccg tacgggctgt attacgcgcc ggacccgtcc tcgcagatcg
    33421 cctgcaccat cggcggcaac gtggcggaga attccggcgg cgtgcattgc ctcaagtacg
    33481 gcctgaccgt ccacaacctg ctcaaggtgc gcgcagtgac catcgacggc gagatcgtcg
    33541 aattcggcag tctggcgctg gatgcgccgg gctatgacct gctgcccctg gtgaccggct
    33601 cggaaggcat gctggcggtg gtcaccgagg tgaccgtcaa gctcacgccc aagccgcaac
    33661 tggcgcgctg cgtgctcgcg gccttcgacg acgtggtcaa ggccggcgac gcggtggccc
    33721 gcatcatcgc ggcgggcatc atcccggccg gactggagat gatggaccgg cccgccaccg
    33781 cagcggtcga agaattcgtg catgcgggct atccgctgga tgcggaggcc atcctgctgt
    33841 gcgaggcgga cggcacaccg gaagaggtgg ccgaggagat cgagcgggtg cgggcgatcc
    33901 tcgaaggcag cggcgccacc gaggtccgcg tgtcccgcga cgaggccgag cgcctgcgct
    33961 tctgggccgg acgcaaggcg gccttcccgg cggcggggcg gatctcaccg gattactact
    34021 gcatggacgg caccatcccg cgcaagcgtc tgggcgagat gctgcgggcc atccaggcga
    34081 tggagaagaa atacggcatg cgctgcatca acgtcttcca cgcgggtgac ggcaatctgc
    34141 atccgctgat cctcttcgat gccaacacgc cgggcgatca ggacgtcgcc gaggccttcg
    34201 gtgccgagat cctggaactc tcggtggccc tgggcggcac catcaccggc gagcacggcg
    34261 tgggcatcga gaagatcaac cagatgtgca gccagttcgc cacaccggag atccgcctgt
    34321 tccaccgggt caaggcggcc ttcgatccgc tcggcctgct caatcccggc aaggcgatcc
    34381 ccaccctcaa ccgctgtgcc gagtacggtc gcgagcgcat ggggcgcggc gtgcccgatg
    34441 ctcatcccga tttgccccgt ttctgatcgc catggatgcc ctgcctgaag gttggctgca
    34501 atttctcttt gtcgccgtgc ccgtgctgtc cctgatcggc acctggatgg gcctgcgcac
    34561 cgcgggccgc atcgtcgagc ggcgcgagcg ggcgcgtcgg gtgggccagc atccgggatc
    34621 cggagacaca tgtgactgat ccaatccagg aatgggctgc ccgcatccgc aaggccgctg
    34681 ccgacggctc ggccgtgtgc ctgcgtggcg gcggcaccaa ggatttcctg ggtcgcacgc
    34741 cgatgggcga ggtgctggat acccgcggct acaccggcat cgtgagttac gagcccagcg
    34801 aactggtggt gacggcgcgc gccggtacgc cgctggtcga gctggaggcg gcgctggccg
    34861 aggcgggcca gatgctcgcc ttcgagccgc cccgctttgc gccgggtggc accgtggggg
    34921 gctgcgtggc ggccggcctg gccggtccgc ggcgggcggc ggtggggggc gtgcgagatt
    34981 tcgtactggg cgtgcgcctg ctggacggtc gcggcgacga tctgcgcttt ggtggccagg
    35041 tgatgaagaa tgtggccggc tacgacgtcg cacgggtgat ggcgggcagt ctgggcaccc
    35101 tcgggctgct cctggaggtg tccctcaagg tgctgccgcg gccgccggcg gaggcgacgc
    35161 ttgcctttgc gctggacgag ccggcggcac tggagcgggt gaacgcctgg agcggacagc
    35221 ctctgcccgt atcggccacg gcgtggcggg cgggccggct ccacgtacgc ctgtccggcg
    35281 cggcggcggc cgtcgaggcc gcgatacggc gtctgggcgg cgaggttctg gcggacacgg
    35341 cggcacatgg cctgtgggcc gcgctgaggg atcataccga cgatttcttc acggatgacc
    35401 ggcccctgtg gcgcctgtcc gtgccgagcg acgcgccgcc gctggacgtg cccggcgaga
    35461 cgttggtgga atggggcggc gcccagcgct ggctgaaggg cgagcaatcg gcggcggagc
    35521 tccgcgcgcg ggtcgcagca gtgggcggcc acgcgaccct gttccgtcac ggcgatcggg
    35581 cgggcgaagt ctttcatccg ctgctgcctg ccatggcggc cctgcagcgt cggctgaagc
    35641 agaccttcga tccggcgggc gtgttcggac cgggccgcct gtacggaggg atgtgagcca
    35701 tgcagacgca actcgccgat ttcatccggg atacgcccga aggccgcgag gcggaggcga
    35761 tcctgcgcaa gtgcgtgcat tgcggcttct gcacggccac ctgcccgacc taccagctgc
    35821 tcggtgacga actggacggt ccgcgcgggc ggatctacct catcaagcag gtgctggaag
    35881 gccagcccgc cacggcgcgg acccgactgc acctggatcg ctgcctgacg tgccgtgcct
    35941 gtgagacgac ctgcccgtcg ggtgtcgaat acgggcgcct ggcagacatc gggcggctgg
    36001 tggtggaacg tcaggtgccg cgtcagggcg tagaccggct tggccgcgag gccttgcggc
    36061 agctggtgcc gaacaccggc ctcttcggtc tggcgctggg gctcgggcga cgggcccgcc
    36121 cgctgctgcc gcggcttttg gcggacaagg tgagcgcagc cgacccggcg ggcgaatggc
    36181 ccgccgcgcg tcatccgcgg cgcatgatcg tcctggcggg ctgcgtgcag ccgagcctgc
    36241 ggcccgccat caatgcggcg gcggcccggg tgctcgaccg gctgggtgtc tcgctcgtgg
    36301 agcaggcggg agcggggtgc tgcggggcgg tgaaatttca cctccacgat caggagggcg
    36361 cccgcatgga agcccggcgc aacatcgacg cctggtggcc gggcgtggcc gccggccgcg
    36421 tcgaggccat cgtgatgacc gcgtccggtt gcggcaccca ggtcaaggat tacgggcacc
    36481 tgttgcaaca cgacccggcg tatgcagaga aggcggtacg cattgcggcc ctgtgccgcg
    36541 atctgtccga ggtgttggcg gccgaagggg atgcgctgga aaccctgatc gccgggtcgg
    36601 cggccgcggt ggagcggcgc cgcattgcct ttcacgcgcc gtgcagcttg cagcacggcc
    36661 agaagatccg cggtgtcgtg gaaaagttgc tggaggcggc cggatttgag ttggcgcccg
    36721 tgcgggatgc ccatctttgc tgtggctcag caggcacata ctcgctgctt cagccgacgc
    36781 tcgccggacg cctgcgcgcg gacaagctgg acgccctggt ggacagtggc ggtgcgacgg
    36841 tggtctccgc caacatcggt tgcatcgagc atcttgccgc cgggggacga ttccccgtga
    36901 agcattggat cgagctgatc gatgaatatt tcacggccct acgtcagaag atatagcgtg
    36961 tattcagcat gcaactgcaa aaaggttaaa cttgtaaaac gatccggcat ttgtcgtctc
    37021 ggatttcctc tttacgcgag gatttatgaa aatggcgtga tgagggctcc atagcgcgaa
    37081 ggcttgatgc gcaagcaggg tcggtcgtcg tcagatcagg ttttcattgt cataagatgc
    37141 tcgaacagtc gctggcttcc attcctgccc ccgacacggt cacggccaag aagcgttacc
    37201 aggcggaagc ctgtgtggcc aagcacgtgg gggatcgcac cgagcagcag gatcgggtgg
    37261 ccgtctttgc ccatcccgcc cgcaagggta ccttgctggc cgtcctcgcc gacggcatgg
    37321 gcgggcattc gggcggcgcc atggcggccg agcaggtcgt tctgaaggcc aagcagaatt
    37381 tcgaaacctt cgcgcccgcc tcggaaagcc ccgaggatct cctgcgcagc gtcgtggacg
    37441 aggcccatat cgtcatcaag ctgacccgct tcaccagcga gcaggatccc cacagcacgg
    37501 ccgtggtgtt catgatgcag ccggaccggg ccgattgggc gcactgcggg gattcccgtt
    37561 tttatcattt ccgaaacggc aagctgatga cccgcagcgc ggaccattcc ctcgtggagg
    37621 agttggtgcg caaggggcgg ctggacgccg aaagtgcggc gcgccatccc aatcgcaacg
    37681 tgctgctgtc ctgcctgggg gccgatcggg agccgagggt cgatctcggg catgtgtcac
    37741 ccctgcaggg gggggacgcc ttcctgctct gctccgacgg gttgtggggc tatatttccg
    37801 aagacgagtt cggggacgtg ctggcgtccc atacgccgcg ggatgcggcg tccacgctga
    37861 ttgaaatggc ccggcatcgg gcacgggggg gcggcgacaa catctccctc gccatcgtca
    37921 agctcgtgga gcttccgccc aagaccgttc ctccgcggga gagctgaccc gtcccgcgtc
    37981 tgttgcgttc tactgcgtct tcagccagtt ccggcgccag aagctggcgg ccatgaaggc
    38041 tgccgcgccg cccatcagtc ccatggccac ccagaatccg tccgcatcgg cgaggagcgg
    38101 catttcatgg aagttcatgc cgaagatgcc ggcgatcagc gtggcgggca tgaacagggt
    38161 ggtcagcacg gtgaggatgc ggacttcggt gttgaggcgg ttgctcacgc tggacaggta
    38221 aatgtcgagc aggtcggcca gcaggtcacg cacggattcg agggattcga ggacgtgcac
    38281 ggtgtggtcg tagatgtcgc gcaggtagag gcgcgtgtcg gcggtgaaga agtcgttgtc
    38341 cgcccgggac agggtgttga gcacttcgcg caggggccac acggcccggc gcatgctgcg
    38401 ggcctcgtgc ttgacccggt tgatctctgc cagcagtgaa ggcgtgggcc gggtgagggc
    38461 gctttcctcc agctcctcgg ccagatcgct gagatggtcg agcatgatga agtagcggtc
    38521 gaccagcgtg tcgatcaggg cgtaggcgag gtagtcggga ccgtgggcgc gcatggcgcc
    38581 ggtctcggca cgcagacgtt cgcggacggg ttcaaaggcg ccagtggcgc gttcctggaa
    38641 ggtcaggacg aagttgcgcc cgagcacgat gctgatctgg tcggatgtca gacagcgcgc
    38701 gggggcgtcg tagtcgaagt aacgggcaac gaggtagagg tattcggcaa attcgtccac
    38761 cttgggccgc tgctgcgtgt tgaggatgtc ttcctggatc agcgggtgca ggcggaagcg
    38821 gcgtccgatc tcggccatga tggccgggtc gtggaggcca tagacgttga gccagatttt
    38881 ttcccggctg cgggtgaaat tgcgggattc ctcgatggaa tcgaagcgga tttcagatat
    38941 gccggtagaa tcgtactcca gcagggtgat gctgggtttt tcggtcttca cttcgccgat
    39001 atggacgagc gttcccggcg gaaggcccac ttttcggagg ggacgggttc gtttcttgct
    39061 aggtttcttc atgttttgcc cggattccgg ctaaaaatca ccggaatctg tgtcagaggg
    39121 tgatcgcgcc gtccggaagc tgctccggat tgttacattc cccacaaact ctgtcgttcc
    39181 gactccctcg cgggacgata gcagaattcc aactacctac tataaggagg ttccatgcct
    39241 ctggtcatcg gtgtgaccaa agagttcacg ccgggcgagc gtcgcgttgc gctcgttccg
    39301 gacgtggcca aaaagtatca gggactcggt gcaagcctct tgctcgagtc gggagcaggc
    39361 gagccggcct acttccggaa cagcgactat gccggctcgg acgtggcgga cgatgccgcc
    39421 gcggtgctgc gcaaggccga cgtcgtgctg caggtccagc ccctggggct ggacgcgctg
    39481 cgcgaactgc ggccgggcag cgtgctggtg gggcttcaac agccttggtc cagcgcggag
    39541 cgcatcgcgg tgctgcggga acgcaacatc acctgtttcg ccctggagct cctgcctcgc
    39601 atcagccgtg cccagagcat ggacgtgcta tcgagccagg cggccgtggc gggctacgag
    39661 tgtgcgctga tcgcggcgga ccactgtccc aagttcttcc cgatgcttac gtatgcggcc
    39721 ggtaccatcc gtcctgccaa ggtgctggtc atcggtgccg gcgtggccgg tctgcaggcc
    39781 atcgccaccg ccaagcgcct cggcgccatg gtctcgggct acgacgtgcg gccggaaacc
    39841 aaggagcaga tcgagtctct gggggccaag ttcgtggata ccggcgtgtc cgcgtccggc
    39901 agcggcggct atgcccgcga gctgaccgac gaggaaaaga agcagcaggc cgagaagctc
    39961 ggcaaggcca tcggcgagtg tgatgcgctg atcaccacgg cagcgattcc gggcaagaaa
    40021 gccccggtga tcgtctccgc cgagatggtg gcgcgcatga agcccggttc ggtggtggtg
    40081 gacatggccg ccgaaaccgg cggcaacgtg gccggaacgg ttcctggtga gaagacctgg
    40141 gtgggcgaag tgctcatcat cgggcccacc cacatcccga gccgcatgcc cgtgcatgcc
    40201 tccgagatgt acgccaagaa ccttttcaac ttcatctcgc ccttcatcaa ggacggggcc
    40261 ctggccctgg actgggaaga cgaggtgatg gccggtgcct gtctgaccca cgcgggcgag
    40321 cttcgccacg cgggcgtcaa gcaggccctc ggcctgtaag cgggagccga ccatggacgg
    40381 atttgtcgca ctgtatatct tcatgctcgc ggccttcacg ggctacgagg tcatttcccg
    40441 cgtgccggtg atcctgcata cgccgctgat gtcgggttcc aacttcgtgc acggcgtggt
    40501 gctggtgggt ggcatgatcg ccctcggcca ggcccagacg cccctcgagc aggccatcgg
    40561 cttcgtcgcc gtgctgctgg gcgcggccaa cgcggcgggc ggttacgtgg tgaccgagcg
    40621 catgctggcc atgttcaagt cgaaggagaa gaactgatgg cgatcaagtg gctgattgac
    40681 gcgagctact tcgtggccgc ggtgctcttc atcctcggtt tgaagggcat gagctcgccg
    40741 gtgacggcgc gcaagggcat tgtctgggca ggcgtgggca tggtgctggc caccgtggtc
    40801 acctttgcca cgccgggcat gcagaactac ctcctgatgg tgatcgcgat cgccgtgggt
    40861 ggggcgctgg cctggatctc cggcaagaag gtcgccatga ccgacatgcc gcagatggtg
    40921 gcgatctaca acggcatggg cggtggcgcc gcggcggcca tcgcggcggt ggaatttgcc
    40981 cgcggcgaga tgcacggcgc cgtggtcacg ttgctcgcca cgctgggggc gctgatcggt
    41041 gcggtgtcct tcgccggctc cgtggtggcc tgggccaagc tccagggcgt catgaagaag
    41101 gccttccgcc tgcccgccca gaacgcggtg aacgtgctgc tggcgctgct caccgtgggt
    41161 ctgggggcgg ccatcgtgat cgcgccggcg gtcgatccga cgctggtggg ggcgttcttc
    41221 atcctggcgc tgatcctcgg caccatcatc acgctgccca tcggtggcgc ggacatgccg
    41281 gtggtgatct ccctgttcaa cgccttcacc ggtctggccg tgggcttcga gggcttcgtg
    41341 atgggcattc cggcgctgat catcgccggc atcgtcgtcg gcgcggccgg tacgctgctc
    41401 acccagctga tggccaaggc gatgaaccgg cccatcagca acatcctctt cgtgccgatg
    41461 gcggccagcg gcggtggcga ggcggtggaa ggcgagcaga aggaagtcgg cgccatggac
    41521 gcggcggcga tgatgcgcta cgccagcaag gtcatcatcg tgccgggcta cggcatggcg
    41581 gtggctggcg cccagcacaa ggtctgggag atgaccgaac tgctggaaga ggccggcgtg
    41641 gaagtcagct tcgccatcca cccggtggcg ggccgcatgc cgggccacat gaacgtgctg
    41701 ctggc
//
LOCUS       DAOQPA010000016        39719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_405_length_39719_cov_19.022060, whole genome shotgun sequence.
ACCESSION   DAOQPA010000016 DAOQPA010000000
VERSION     DAOQPA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 39719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39719
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_39719_cov_19.022060"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5491)
                     /locus_tag="PKX01_03955"
     CDS             complement(<1..5491)
                     /locus_tag="PKX01_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HMZ75308.1"
                     /translation="MNRIYRSIWNEVSRSFVAVAETVRSQGKPASSSVVAAAECVAPS
                     ASGDPTCSSRPGSRTLRSALRPLALEQRFMFDGAAVATVDSHAHDSTAAEAAAAAEAL
                     AAQAAQAATPARESDKASPADGPTLIRAVDPALNGGRREVAFIDSQVVDYQSLVSGIP
                     QGIEIQLVDGTHNGLEQIAQWAASHHDYDAIHILGHGSSGALTLGTATLDGDTFGAYQ
                     DTLGTIGGALKAHGDILLYGCNVAATTAGTDLIQSLARATRADVAASDDATGATRLGG
                     DWVLESTVNHIETRSLQFAQYDGLLANPDVWQWGAPATVVSGGQVRSLPTDTFYIRPN
                     GTTSTVLKLPSNQYFGYYEINSGGPGYFEYSTNNGSSWTILANHWSAFAPPNALVRFH
                     ASGGSVSEVDFGAVTCLDATNTTTAANSGFYNGGGYKVRIDTAPTDIASNNSSGLLVS
                     DTPLGSDIATLTPTDTGTSLGGYWAIDSQTVSGLFGLSFNAATGNTAHLVLASTSNLP
                     SEGQTASVTVHFYDALQTDVTGAPIGGQGVTKTYTFTIKGPSHDLNFGNDIAVNPLAG
                     DTVNQSEPAITTLSNGNFVVTWADISNGGIYSRVFDSAGTAQGAPITIDAGSSATQPS
                     ITALAGGKFAVVYGDATTYDIRYKIIDGATGSASSNGLIEAYAVDGGGNQTGGWDGYD
                     PIGVAASADGSQFSVTWNLGNYTTVKTAVLSDSGTPNGAIVDLSGVTAGFAPVTAVLA
                     NGDVVTASIDGNVWAGFNLTVGGALVSGGPVGDNGTGVSMATLDGGGFVVAWSKGDGS
                     HVYAQTFSNAGTPTSSILQADSQGTAMSAARVSALGGGGFVVVWQSDGLDGSGTSIQG
                     RRFDAAGSAVDAQEFQVNEYRYGDQSTPVVTPLDNGAFATAWVDSATSGKTTGSDIEA
                     RVLSTAPSITSATYDAATGVLNVTGDNIPANASGADIVAALFTLGGEGSATYTLTDTA
                     DVERTSATQFVLTLSATDRSRINQILNRNGTSASDGSSYNLAAADNWAAGATPSANIA
                     DTTGNAITVSNVALPAITSATYDAGTGVLTVTGSGLTHHAGSANDIDVTRLTLTGAGG
                     ATYTLTASTSDVDITSDTSFTLNLGSTDKTAVDSLLTANGTQVGGVTYNLAAADNWER
                     GADSSLNIADTVNGVLVSGLNPAPTITPPADINLADTGATDTFAVQTGTLSATDADGI
                     AAFGIQSPTSTNAGFTHGGITYDVARSGTYGTLYLVSTGADAGRYAYVPDAGAINGLT
                     AGATPTDVFTVTATDANASPATGTATLTVHLTGANDAPTLSATGLSPTVTENGSAVTL
                     FSGASASAAESGQNLNQLILTVSNVADGSSELLQVDGSTLALTHGNSLTTASHGVSAS
                     VSLSVGVATVTLDKAGGLSTSVMQSLIDGLGYRNTSESPSTSNRVVTLTSLRDTGGTA
                     NGGQDTTVLAIASTVTVVSVNDAPTDITLSASSLNLSAATASAPVATLGVADLDTSDT
                     HSYALVSGSGDADNGRFVLSGNTLQVGGAALSAGTYSLRLQVTDNGGLSYTKAVTVTV
                     VDDVAPTLDAPGSTPAGDASGVAPSANLQLHFDEAIAFGTSGTITLRNVTTGTVVQTW
                     NVATPGDLGSGNGQIAISGSTLTLNPTATLDEASRYAVRIAPTALRDTAGNAFAGIAD
                     DSTYRITTGVTDSAPPALLAIQRASPSTELTRSGSVSFTLRFNEAVTNADLADFALST
                     SGTATGTLGSVTGNGTDTLTVTVTGVSGTGTLGLSLGGGHTITDIAGNALPGGNPADT
                     ELYHVDRDAPGLVSINRVTDDDVLASGTAQFIVVFREPVTQVDVGD"
     gene            complement(5511..6308)
                     /locus_tag="PKX01_03960"
     CDS             complement(5511..6308)
                     /locus_tag="PKX01_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75309.1"
                     /translation="MRLRSIALIINALLVTPAWSQTPPPRSAAAPAPVASNPGSALEN
                     REIRAQLAPRRYTTLAAEIGAKVSHLPVPEGGAFKAGQSLVQFDCSLQQAQLAKARAA
                     LAGADKTWQANRRLADLNSVGKVELETSEAEVLKARAEVSANATLVGKCAIAAPFAGR
                     IAEQKVREQQYVQPGQALLEILDDSALELEFIVPSRWLAWVRTGTAFQVRIDETGRSY
                     PAKVQRIGARVDAVSQSVKLNAVIDGRFPELIAGMSGKVQMTPPANP"
     gene            complement(6316..7872)
                     /locus_tag="PKX01_03965"
     CDS             complement(6316..7872)
                     /locus_tag="PKX01_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ75310.1"
                     /translation="MHRTNLRSTTRPLTLSALAISLLLLSGCASMKPEPLQPAEIVST
                     VDADRARLMQGVEPLTGVLSLEEAIARAIKHNAERRYRIMEEALAQGTFDASTYDLLP
                     RLVASAGYRDRNNDLISHSTDSVTGAPSLANPFISTGRTATTTDLTLSWSLLDFGQSY
                     YAAKQNADRLLIAQERRRKALQLLVQDVRTAYWRLVAAQSLHEAVGQGIREAEAALGD
                     ARKAEKEGLRNPMEPLRYQRTVLENIRMLETVQQELSTARTELAALVNLPMSTEITVA
                     PPSAVLQKAWLDLPVARLEEQALMRNADLREGILNSRIARQETRRTLLKLFPGLSFNY
                     GTKHSTDSYLINQTWNEAGVQLSFNLFNVFSAPAQMRLSEAGVALADQKRLVTQMAVL
                     TQLHVARLQYANAIHQFERADEIADIEARMAGHARNQSDAEKQSRLDRVSQSTSYVLA
                     QLRRYQALSNAQSAASKLQATLGLEPVVDAGAERPLPELTDSVRKALDAWQQAALPDA
                     GGSATGRDAP"
     gene            complement(8333..9535)
                     /locus_tag="PKX01_03970"
     CDS             complement(8333..9535)
                     /locus_tag="PKX01_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ75311.1"
                     /translation="MEQITTTSNVVFVLLGAIMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKILTDFGVSALAYFFIGYTLAYGVNFFSSAEVLAQKSGYEMVKFFFLLTFAAAIPAI
                     ISGGIAERARFHPQSVATFLLVGFVYPFFEGIAWNDNLGLQPWLEAAFGAKFHDFAGS
                     VVVHAVGGWIALPAVLLLGARHGRYTKDGNISAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQTMDKVSGLVALNSLMAMVGGTMAAMVLGKNDPGFIHNGPLAGLVAVCAGSDLMH
                     PVGALVTGAVAGVLFVQLFTITQNRWKIDDVLGVWPLHGLCGAWGGVAAGIFGSQALG
                     GIGGVSLAAQIIGTLAGIVVALAGGTVVYGGLKALIGIRLDQEEEWEGADLTIHKITA
                     TPDRETHW"
     gene            complement(10089..10424)
                     /locus_tag="PKX01_03975"
     CDS             complement(10089..10424)
                     /locus_tag="PKX01_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75312.1"
                     /translation="MPLGQLIHARTREHRVPHATTADGTSPRAGGPGNTGLPGFRITS
                     QALIRESSHSQIVDGPEDQVNLHNRRVHQSIVDADLSTDDGRLAARARRAHRAERPDD
                     HRATWSALT"
     gene            10777..11451
                     /locus_tag="PKX01_03980"
     CDS             10777..11451
                     /locus_tag="PKX01_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HMZ75313.1"
                     /translation="MKDNVSKNGSDTRERILDAAEVLFMEHGFDGTSMRMITVQAAVN
                     LAAVNYHFGSKELLIQDVFRRRLTDLNKARLEALDALERQAGDAPVKPSRIVDAFFGT
                     ALRMAADVDGGGHTFMRLLGRTYTEPNEFVRKFLAEEYAECVERFLAALYRALPEVDR
                     HEILWRFHFMMGAMSYAIAGTDALQLVTGKFDDENPSRLAPRLMSFLLGGLRAPLADF
                     GPQPDA"
     gene            11514..13988
                     /locus_tag="PKX01_03985"
     CDS             11514..13988
                     /locus_tag="PKX01_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ75314.1"
                     /translation="MWWFLVFIPPLVVALAYGRAPFFAWVGAMLAWLAAWTAFGGLPP
                     GAALGVGAVVAVAGALFLVPALRRGLVTAPIFRAFRAALPTMSQTERDALEAGTVWWE
                     GELFAGRPDWRTLLAYPVPRLTDEEQRFLDNETAELCRLTQDWDCTQRQDMPAEVWQY
                     IKDKGFLGMIIPREYGGKGFSAFAHSQVVTKLSTRSSAPAVTVMVPNSLGPAELLLHY
                     GTPEQKAHYLPRLARGQEIPAFALTSPWAGSDAASIPDAGVVCRGTWQGREVLGMRVS
                     FDKRYITLAPVCTVFGLAFRLFDPDRLLGDVEDLGITCALVPHDHPGVKIGRRHLPLN
                     AVWMNGPIQGQDVFMPLDFIIGGPTMAGQGWRMLMECLAAGRSISLPGSNTGMQKMTA
                     RAVGAYARVRYQFKTAIGRFEGVEEALTRIGANTYLCDAARVMTAGAIDLGEKPSVVS
                     AIVKYHVTERARQTVNDGMDVIGGKGICLGPQNFLGRAYQQIPVGITVEGANILTRSL
                     ILFGQGAIRCHPYVLKEMNAAREGDLAAFDHAFWRHIGYTLSNAARALVTGLTGSHFV
                     SVPDGVAPETRRYYQQLSRFSAAFAFLSDISMGTLGGALKRKEKLSARLGDILSLMYL
                     ASATLKRYEADGRPTADAPLMHWAIWDCMFKAQNAFEGAIANFPNRFFAMLLRRLVVF
                     PLGRPYVVPSDRLGHEVARLLIEPSPTRNRLTSDVYLPTDVEEPVGALEAALLATVDA
                     EPVEARVRRAQRDGQFRPTVSVGGGVDAVYDQALAAGVITAAEHALIKRRGVLRDKVI
                     RVDDHPYDFGLRDALATVDVPARQAA"
     gene            14004..15284
                     /locus_tag="PKX01_03990"
     CDS             14004..15284
                     /locus_tag="PKX01_03990"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HMZ75315.1"
                     /translation="MTTPVYIIDGARTPFLKARNAPGPFAASDLATAAGSALLMRQPF
                     APDQLDEVILGCASPSPDEVNIGRVVALRMGCGVKVPGWTVMRNCASGMQALDSAIAN
                     LQTGRSQLVLAGGVDALSRAPLLFSDAMVRWLSGWYAAKKFGQKLAHMRHFRLGHLAP
                     VIGIMKGLTDPVVGQLMGQTAENLAWRFGITRDDMDAFAVESHRRVAAAQDAGHFAEI
                     VPLIDRDGTLYTADDGLRRDASTSGLAKLKPFFDKHYGRVTAGNSSQITDGATWLLLA
                     SEAAVVRHGLTPLGRIADSQWAGLAPEQMGLGPVHAATPILQRHGLGLHDVDLWEINE
                     AFAAQVIACRRAWQDTGYCREQLGLDGALGALDVARLNVDGGAVALGHPVGASGARIV
                     LHLLHSLRRTGGRRGVAAICIGGGQGGAMLVEAL"
     gene            15284..17215
                     /locus_tag="PKX01_03995"
     CDS             15284..17215
                     /locus_tag="PKX01_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ75316.1"
                     /translation="MDLKHWTLERDADGLAWATLNVADAATNTLGRAVLDELGALLDD
                     LDRQPPTGLAIRSGKPAGFIAGANIEEFTRIDSAEAARTLVNRGWALFNRLAAVTYPT
                     LALIRGHCMGGGLELALACRYRLTVDEPGTRLSLPEVMLGIYPAWGGMLRLPRLIGPA
                     AALDLMLTGKSVDALKAKRLGLADDCVPPRVMDSAARVVLLSGRSPRRLPFGQRLLNG
                     PLKGVVAARARTTVAAKARPEHYPAPYAIIDLWANHGGNALAAPAASPSSLEAIFRSP
                     TAKNLIRVFFLQERLKGFGKDTDFRPQRVHVVGAGVMGGDIAAECALRGMVVTLQDQS
                     AERIAPAIARAAKRFERKFRGKRREARFALDRLIPDPAGHGVARADVIIEAIFENLEA
                     KRTLLADLERRARPDAVLASNTSSLRIGDIATALREPARLVGIHFFNPVAMMPLVEVV
                     RTGHTADAPLRCAAAFVRALDKLPLPVRDEPGFLVNAVLGPYMYEALRCVDEGIAPAT
                     VDAALTAFGMPMGPIELVDTVGLDIAVAAGTALAGADAVLPRTLADKVAAGWLGRKSG
                     QGFYLWQNGKAQKSAPGPLPDGLAERIVLPLRQAVQACVDKGVVADADLADAGVIFGT
                     GFAPFTGGPLHDLASRARP"
     gene            17235..17642
                     /locus_tag="PKX01_04000"
     CDS             17235..17642
                     /locus_tag="PKX01_04000"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ75317.1"
                     /translation="MSDSDSRLPEGKIPVLRVMPMPADLNPAGDVFGGWVMAQVDIAG
                     AIPAKRRARGRVATISVNAFLFKQPVSVGDLVSFYATIVKVGRTSITVDVEVFAERNP
                     ENPVVVKVTEATLTYVAVASSGVKREIPPEEPA"
     gene            17769..19058
                     /locus_tag="PKX01_04005"
     CDS             17769..19058
                     /locus_tag="PKX01_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HMZ75318.1"
                     /translation="MKMKTLARMMPILAVAAATGEASAAGFQLLEQNASGIGNAYAGS
                     AAVAENASTIYFNPAGMTQLKDREFSAGIAVVRPSFRFANENSSAGALTSTGNGGDAG
                     STGFLPNLYASWALNKDLYVGLGIGAPFGLKTEYDNPWIGGAQSVRFEIKTVNINPSV
                     AYRVNEKVSIGAGINWQRIDVDYTRVLGTNSARLGVTVPFPLNTSTGTFSANDSTWGW
                     NAGALFTLSETTKVGVSYRSKMRYTVKGDLTVQGPYAPLNAARSGPANATVELPDVFV
                     LSVVQQLDPRWTMMGDISWTGWSSIPHVDIINSNSGTTAQRLDTEFRDTWRMALGATY
                     KVNDAWKAKFGVAYDQSPVEDPHRLVSLPDSNRTWYSVGGQWKTPAGSLVDFGFAYLR
                     VNTSPIHNDQSSALRGVVDGHYNSNAFIAGAQYSLAF"
     gene            19124..19801
                     /locus_tag="PKX01_04010"
     CDS             19124..19801
                     /locus_tag="PKX01_04010"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HMZ75319.1"
                     /translation="MGRLEHPQPDAKTRILDAAERLFMERGFAATSVRMITGAAGVPV
                     ALVNYHFGSKQGLMEAVYARALGNRGSRVSYLDRLEAEADGRPIAVNVLVDAFLSSAL
                     RLTRKDSIPGEVFKQLIGRAFYEPGPGTEAFFPDEYREAVDRYRQAFMRALPQLSQED
                     VVWRMYFLVGIVAYAMAGKDVMRMTETYALADQGQPEAILARLRPFVVAGFLAPPGAL
                     PSIAEIP"
     gene            19972..22356
                     /locus_tag="PKX01_04015"
     CDS             19972..22356
                     /locus_tag="PKX01_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HMZ75320.1"
                     /translation="MSRLIIRKVAVLGAGVMGAQIAAHCANANVPVILFDLPAKEGDP
                     NGIAKKAVDSLKKLEPAPLATRDRAAAIELGNYGADLEKLRDCDLIIEAIAEKMEWKL
                     DLYAKVAPYVGDQAIFATNTSGLSINTLAEGFPEAARSRFCGIHFFNPPRYMALVELI
                     ATRSTHADTLDALEAWLTTRLGKSIVRAKDTPNFVANRVGVFSILAVMHHTARFGLGF
                     DEVDALTGPLIGRPKSATYRTADVVGLDTLAHVVGTMQATLPDDPWFPYYRNPDWLAA
                     LIAKGALGQKTGGGIFRKVGKAIHVLDLAAQDYRPSAGTVAPEVAAILKNRNVAERFA
                     QLRASEHVQAKFLWAIFRDVFHYVAVHLGDIADNARDVDFAMRWGFGWTTGPFETWQA
                     AGWQSIAEAVKADIAAGATMASAPLPAWVFDGRTGVHGPAGSWSAADQTDKARSTLAV
                     YGRQLYPEQVLGEAPADAGVTLWENEGVRLWHLPAKDARIGILSFKSKMHAIGDEVLD
                     GVLEAVARAERDLDGVVIWHEAPFAVGANLQQVAEACKGGQFDVLERTVAKFQQASMR
                     LKHALVPVVAAVQGMALGGGCEFLMHASHRVLALESYVGLVEAGVGLIPAGGGCKEFA
                     LRAAELASRAAGGDVYPFIQNMFNTVAMATVSKSAVQAVELGFAKPSDDIVFNPRELL
                     HVALHRARALAEAGYQPPLVRKDVTVAGRNGIATCEMMLINMQAGGFISEWDYRVARA
                     AATALCGGEVETNAKVSEQWILDVERALFLDLLKSEKTQQRIVHMLETGKPLRN"
     gene            22421..23617
                     /locus_tag="PKX01_04020"
     CDS             22421..23617
                     /locus_tag="PKX01_04020"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ75321.1"
                     /translation="MSKQIQEAYIVAATRTPVAKRNGMFRNVRPDDMLAHVLSAVVGK
                     VPGLDAREIGDVIVGCAMPEAEQGMNVARIGVLLAGLPDTVPGITINRFCSSGLQAVA
                     DAAARIRLGEADVMIAAGTESMTVMPQMMGNKISLNPAVFAKDENVAIAYGMGLTAEK
                     VAQQWEISREAQDSFAVQSNHRAAAAIAAGAFRDEITPYTVRTHVPGAGGVVRVSERV
                     CDTDEGPRPDASVAGLGKLRPVFAARGSVTAGNSSQMSDGAAAVLLMSEAAVKRYGVT
                     PIARFVSYSVAGVPPEIMGIGPIEAIPRALKLGGVAQSDLDWIELNEAFAAQALAVMK
                     QLDMDPARVNPQGGAIALGHPLGATGAIRTATLMSAMQRGQARYGMVSMCIGTGMGAA
                     GIFERV"
     gene            23740..25386
                     /locus_tag="PKX01_04025"
     CDS             23740..25386
                     /locus_tag="PKX01_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HMZ75322.1"
                     /translation="MMPTGDGPYAYPLLIKQLLRMPLVHAPDQEIVYRDQVRHSYREL
                     AARIGRLGSALSRLGVARGDTVAVADWDSHRYLEAYFAVPMLGATLMTVNVRLSPEQI
                     AYTLDHCEAETLLLNVDFLPLLEGIRGRLPKLKRLILLADGVCDPVAAGALAEYEALL
                     ATGRPDHAFPDFDEHTRATTFYTTGTTGLPKGVYFSHRQLVLHTLAGLAGLASAPGTQ
                     RFDRGDVYMPLTPMFHVHAWGLPYVATLLGVKQVYPGRYVPDEILALRAREGVSFSHC
                     VPTILQMLLLAPSARDMDLSGWKMVIGGSALTAGLATQAVARGLDLFAGYGMSETCPI
                     LTLAQLKPEELELPPEQTVSARCRTGLPLPLVDLRVVDADGTEVPHDDHAQGEVVVRA
                     PWLTQGYTKNPEATAQLWAGGWLHTQDIGRISPAGSLQITDRIKDVIKSGGEWISSLE
                     LETLVSRHPAVAEVAVLGVPDARWGERPLPVVVRRAGCEAVDAEAIIAHLRACAEAGQ
                     ISRFAVPDRVVFVEALEKTSVGKLNKRLMRERYAVADGPR"
     gene            25460..26257
                     /locus_tag="PKX01_04030"
     CDS             25460..26257
                     /locus_tag="PKX01_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ75323.1"
                     /translation="MTSPVTLDLQDGIATLTLNRPTALNALSVDMMKALAEATGQLQR
                     RTDLRAVVVTGSGEHFMAGGDLKDFATHLHLSAESRIATFRALIEQYINPSVEALAAL
                     PVPVIAQVRGACAGFGLSLVLGCDMAVAAGNAYFTTAYASIALSGDGGVSWFLPRILG
                     RRKAFELLLLADRFGADEALRLGVVNRVVPPEELEAATDALVQRFVKGPREAYAEMKR
                     LLVASPEQSLSAQLQLEAEAFARSSARPNFDEGIRAFLEKRKPGFNA"
     gene            complement(26247..27020)
                     /locus_tag="PKX01_04035"
     CDS             complement(26247..27020)
                     /locus_tag="PKX01_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HMZ75324.1"
                     /translation="MFFLKKIAAALLLPPFLPFLLIIAGLLLLHRQPRTGRLLAWCGV
                     VTGIALSLPASVGLLVTPLERDAALDVRDLPAAEAIVILGGGTRHYAPEFGGQTVNRL
                     TLERLRYGALLARHSRLPVLVSGGSPTPGIAEGILMRRVMEEEFRVSVRWVESQSLDT
                     AENAAFSVPVLRAAGVRRVLLVTHAAHMRRALEAFEAAGMHAIPAPTAFFHGAGPADE
                     VVDFLPGMNAAFAGGYAAHEWLGLAARRLTDLGGGLIRR"
     gene            27179..27811
                     /locus_tag="PKX01_04040"
     CDS             27179..27811
                     /locus_tag="PKX01_04040"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HMZ75325.1"
                     /translation="MIEIGVREGIWIAIGVLTLYLAFVLIRLALHRARPLAEEPQTLV
                     VDAEAPAPQGVAPAALTPSGEDVASIRTPAPTEDADVGDIEPLDDYADPRTGRGESSA
                     GEQPDSLPTFDLIEFFQLEVQQLRRDVAQLRGEQDQLRREIASVRRDLDEIQVADGRS
                     HFVRPVSPQHSEAMVLAERGMTAAQIADRCGIAVAEAELVCALARREVHE"
     gene            27808..28653
                     /locus_tag="PKX01_04045"
     CDS             27808..28653
                     /locus_tag="PKX01_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ75326.1"
                     /translation="MTDERNNPPVQDEKDDSRRQLWTRAAVAVGLIGLLLGSLIVFDE
                     ASRPPEAEEAKVPAKPIGPAPVAGRESEPPPDVVRAGGEAADQASSAPPLPVAADQGE
                     PPKAARKADDAADETRPVVGAGARTVLPESPAVASSRSVTLKEDTLRGGVAPLAATPA
                     AAPAGAPSAPAGAPQARREPVGGGLASPPGSAPAAVAPQAEVRPPADGRAAPARGYVV
                     QVGGFMEPAAAEDLRARLARGGVPVQLESRVMVGPFADRREALAAQSRLREQGYAPGV
                     IQPSR"
     gene            complement(28676..28900)
                     /locus_tag="PKX01_04050"
     CDS             complement(28676..28900)
                     /locus_tag="PKX01_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75327.1"
                     /translation="MGTFRLILAHARAPQQSTVVAQHVDPVWLKQRGYEIARSLGDGS
                     ALWGRRADAGNLSGLELNCRGGHALRIEAA"
     gene            complement(28971..31172)
                     /locus_tag="PKX01_04055"
     CDS             complement(28971..31172)
                     /locus_tag="PKX01_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ75328.1"
                     /translation="MVIPGERPFFPPETGNRGAPDPLIRILVVDDQPSLRRSVVALLQ
                     GDDRLIEDCGSVAETLVRLGAASYDLIILDYRLPDATGLAVMNWLSSHHRNESVIVIS
                     GEDSMEAAIAALRRGAQDFLRKPYDPDQLRRAVQSVLGKRQQERSHRLTLQRLQSSEH
                     MHRYLVESSPDLIFTTTPDGRFNYINPRIESLLGYRRNSLIGQHFSEIVHPEDLERTR
                     QTFHERRTGQRAAANVEVRLTRSPFGPDQDGEAGPLPFVLNAHGIYHDEDGTTPGEYL
                     GTYGVARHAPPLRLTALAGNPPLQRDPLTRLPGRELLFDRLDLAVAQAKRRKGQIAVM
                     QVDVDRFKDVNERFGQGEGDTLLCAIARRLQRCLRRGDTLSRQGGDEFTILLPDVRGK
                     DNAQAIAGKIHQAFEQPFSLGKDEVRLNCSIGVALYPEDGDDAESLTQHAAIAMFQAK
                     RNGGESHEFFEPGMHSAYRARISLETELRLAISNRELELFYQPRVSLSRNQVTGMEAL
                     IRWRHRNHGLVAPSRFLQLAEEVGIMPDISRWVLETACAQLSAWRRLYPELRLATNLS
                     LRDFDRADLNDAIYKALAQHNLPPDSLELEITENLLLEDHEFLAAKVQGLRDMGVGLS
                     IDDFGTGLSSLSLLHRFPVTRLKIDRSFVRNINGQGSHPVINAITGIARGFDVQVSAE
                     GVERGDQVSVLETAGCDEMQGYFFSKPLGAEAATRMLQDFRPAAMLQTAPG"
     gene            complement(31222..33270)
                     /locus_tag="PKX01_04060"
     CDS             complement(31222..33270)
                     /locus_tag="PKX01_04060"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ75329.1"
                     /translation="MPALPECAATLLKVVAEGTTSSEDISLRICRDPILFLEILTTAP
                     LGRDERSTPLKAVERRIAELDPGLLQAWFLRHAVPAPGQRTSIGSLRNLSLHSQLVAE
                     LAFHLARETAYPTPEDAYVAGLLHDVGQLWLAEHQEGYVTMRAALSDEKALSAAEWER
                     YGADHSTISAELAHQCGLGALVADAILLHQTSPVQLRSAHLLVRLIAAAEELASYRQT
                     QSALSLDIASHLAGLDGSVLVSLLAETGPQVNDAASDFGVAIEPAMLALPWALGPDVA
                     PAPEAGTTGISRAALNAFARGTFASAEEARNGLARAARLLLGLDAPALFVRSDNGLAL
                     VGCASATHPSLDQMCVTLADDTSAIAEAARSNAETRYRCDERPPGRSAIDWQVSRWLG
                     SDGMLCVPWKNHHDAGVAVFAFASADSDPPDSTRALRRDLVLSATLRLSEERASQHLR
                     DDLEQTISRRYAEHARRLAHEASNPLTVIKTYLGIIDERIEDAAIREQLGVLNDELDR
                     IGNLVRRAPDFREPAADGPAQSGVTEILHDLRALYGDALFARRGIQFEVRTTPDLPSA
                     AIAPDALKQVLVNLFKNAAEAIPEGGKLVVTVGTGINANGTSCLEIRVVDNGPGLPPG
                     RIETLFAPVPGGAGRTEGGIGLPVVRDLITAHGGTVLCRSQLGSGTVFQIFIPLAGTP
                     "
     gene            complement(33515..34654)
                     /locus_tag="PKX01_04065"
     CDS             complement(33515..34654)
                     /locus_tag="PKX01_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ75330.1"
                     /translation="MSHTALITREPVINKQYKITANRLVVHAKSVGEVVQTLDGVADV
                     WPATHPVFISLYRLVPTPDLLEWIAPANAMVEIPTQAIAHPLTQQLIPQLRAQGVSLN
                     LTWYHPDVTLPPDVDWRFVIMDAHKHPAPLDPPGLSIAWGLEDFPAFRAAIGSGYDGA
                     SGWFFLKGLNAGGKLAPSHAQIVRLLNLVRRNAEVKEVETVLKQDVALSYKLLRYINS
                     AGFGLMCEIQSFRHAVTILGYDKLHKWLSVLLVSASRDPSAPALMQTAIARGRFMERI
                     GEAFFDRNQLDNLFITGAFSMLHLLLGASLHSALEEMHLPEPIADALLSGEGVYAPFL
                     KLARALEGFDDKHLTELAADLHISADVINRAHLEALGFADSLQFG"
     gene            complement(34680..35711)
                     /locus_tag="PKX01_04070"
     CDS             complement(34680..35711)
                     /locus_tag="PKX01_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HMZ75331.1"
                     /translation="MSFQVTLQPGAHQFAASADETLLQSALAAGLLLPYSCRDGACGV
                     CKGKVVSGRVDLGRYSESSLTASERAQGMALMCCARPLTDLELEVRQVNLVGEIPVKK
                     MPCRVQKLQRLCDDVIVLDLKLPASERFQFRAGQYIDFLLADGKRRSFSIANAPLDGA
                     DHLEIHVRLVPGGQFTTHVFERLKEKDILRFEGPLGSFGLQPGAAKPVVLLAGGTGFA
                     PIKSIVEQAIADASTRPMTLYWGSRDRAGLYQFDEARAWEARLPGFRFVPVLSDVPAG
                     EAWDGRRGLAHHALMEDLPDLSGHEVYACGSPAMIDAARSDLVARCDLPPDAFFADAF
                     SFSNDARGA"
     gene            35748..36620
                     /locus_tag="PKX01_04075"
     CDS             35748..36620
                     /locus_tag="PKX01_04075"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ75332.1"
                     /translation="MKTKRVLIVGSGDVARRLIPWLARRCRVFAVIRRPEEREALRAL
                     GAVPLVADLDDRASLDRLAGVADAVLHCAPPPGQGSDDPRTRSLLAALSRRASLPRRL
                     IYISTTGVYGNCGGAWLDETRPVAPASSRGVRRVAAERRLRSFGTRSGCGVSILRAPG
                     IYAVDRLPVERIRRGDPVLAADDDVFTNHIHADDLARLLGLSLFRGRANRVYNACDDS
                     QMRMGDYFDAVADAFALPRPQRLPRAELIAQVSPLTLSFMSESRRIRNDRIKRELRVR
                     LIHPTVADALKRLA"
     gene            36657..37067
                     /locus_tag="PKX01_04080"
     CDS             36657..37067
                     /locus_tag="PKX01_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HMZ75333.1"
                     /translation="MLIVKALHIIFVTSWFAGLFYLPRLFVNHAMVTDPATIERIALM
                     ERKLYRFMTPLGILAVLLGMWLWFGYGFVGGWLHAKTALVTLLIVYHLYCGRLLRAFA
                     EGRNTRSHVWYRFFNEIPVLVLFAVVFLVVLKPF"
     gene            37090..38262
                     /locus_tag="PKX01_04085"
     CDS             37090..38262
                     /locus_tag="PKX01_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HMZ75334.1"
                     /translation="MDFFAPVPRGLEPLLVDELRSIGAVDPKALPGGVQFSGDWAVCY
                     RANLESRLATRVMLQVASGRYRSEDDVYRIAYAPTWARWFTADQTLRVFVTAHKSPLR
                     SLEFTTLRIKDAVCDHFRSVAGRRPNVDTKAPDVRIHAFLSADTVTLYLDTSGEPLYK
                     RGFKPAAVEAPLKENLAAGILRLTGWQPGSEALVDPMCGSGTFLIEAAQMALDVAPGL
                     GRRFGFEKFKNFERDQWLPIRQAAEARRQAPRPLPIHGSDILEAQERKSIVNLRSAGL
                     DGCVTVSRTDMLELAAPAASGVLVANPPYGVRLSDMSELRDFYPRLGSALKAHWAGWR
                     CYFFSGDPELPKGVRLKASKRTPLFNGAIECRLYEFAMVAGSARKPKGEGGAADAD"
     gene            complement(38392..39525)
                     /locus_tag="PKX01_04090"
     CDS             complement(38392..39525)
                     /locus_tag="PKX01_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HMZ75335.1"
                     /translation="MERDQALKFMHDLLKLMMQKNGSDLFITTGFPPAIKVDGKITPQ
                     SNQVLSAAHTAELARVIMNDRQAAEFEATKECNFAISPAGIGRFRANAFIQQGQVGMV
                     LRTIPQRIPNFTELGLPPVLKDIAMSKRGLVVFVGGTGTGKTTSLAAMVDYRNEKSYG
                     HIITIEDPVEFVHPHKNCIVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETM
                     DYAIAFAETGHLCLATLHANSANQAIDRIINFFPEERRAQLLMDLSLNLRALISQRLL
                     PMCDRKGRVPAVEVMLNSPLISDLIFKGEIPGIKDVMKRSAELGMQTFDQSLFDLYER
                     GLITYEDALRNADSMNDLRLQIKLKSRRGDNDMLSGIQNLDIV"
     gene            complement(39603..>39719)
                     /locus_tag="PKX01_04095"
     CDS             complement(39603..>39719)
                     /locus_tag="PKX01_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HMZ75336.1"
                     /translation="QNLGMQTLDQSLTDLIRRNQISAVEARVYAANKDSFTG"
BASE COUNT         6147 a        12566 c        14206 g         6800 t
ORIGIN      
        1 agtcgccgac gtcgacctgg gtgacgggct cccggaatac gacgatgaac tgggctgtcc
       61 cgctcgccag cacgtcgtcg tccgtcaccc ggttgatgga gacgagcccc ggcgcatccc
      121 ggtccacgtg gtagagctcg gtgtcggccg gattgccgcc gggcagggcg ttgcccgcga
      181 tgtcggtgat cgtgtgaccg ccgccgaggc tcaggccgag ggtgccggtg ccgctgacgc
      241 cggtgacggt caccgtgagg gtgtcggtgc cgttgccggt gacgctgccg agggtgccgg
      301 tagcggtccc cgaggtgctc agggcgaagt cggcaaggtc ggcgttggtg acggcttcgt
      361 tgaagcgcag ggtgaagctc acactgccgc tgcgggtcag ctcggtggac ggtgaggcgc
      421 gctggatagc cagcagcgcg ggcggtgccg agtcggtgac gccggtcgtg atgcggtagg
      481 tcgagtcgtc ggcgatgccg gcgaaggcat tgcccgcggt gtcgcgcagg gcggtcggag
      541 cgatgcggac ggcgtagcgg ctggcctcat cgagcgtggc cgtggggttg agagtgaggg
      601 tgctgccgct gatggcgatc tggccattac cgctgccgag gtcgcccggc gtggccacgt
      661 tccaggtctg gaccaccgtg ccggtggtga cgttgcgcag ggtgatcgtg ccgctcgtgc
      721 cgaaggcgat ggcctcgtcg aagtgcagct ggaggttggc gctgggggca acgccgcttg
      781 cgtcgccggc gggggtggag ccgggcgcgt cgagggtcgg cgccacatcg tccacgaccg
      841 tgaccgtgac cgccttggtg tagctgagac cgccgttgtc ggtcacctgg aggcgcaggg
      901 aataggtgcc ggcactcagc gccgcgcccc ccacctgcaa ggtgttgccg ctgaggacga
      961 accggccgtt gtcggcatcg ccactgccgc tgacgagggc atagctgtgg gtgtcgctcg
     1021 tgtccaggtc tgccacgccc agagtggcga ccggcgcgct ggcggttgcc gcggaaaggt
     1081 tcaggctgct ggcactgagg gtgatgtccg tgggcgcatc gttgacgctg acgacagtga
     1141 ccgtggaggc aatggcgagc acggtggtgt cctgcccgcc attggccgtg ccgcccgtgt
     1201 cgcgcaggct cgtcagggtg accacgcggt tcgaggtgct gggcgattcg ctggtgttgc
     1261 gatagcccag gccgtcgatc agggactgca tgacggaggt ggacaggccg cccgccttgt
     1321 ccagcgtgac ggtggcgacg cccacgctga ggctgacgct ggccgagacg ccatgggacg
     1381 cggtggtgag gctgttgccg tgggtgaggg ccagcgtgct gccatcgacc tggagaagct
     1441 cgctgctgcc gtccgccacg ttgctgaccg tgaggatcag ctgattcagg ttctggccgg
     1501 attcggcggc cgacgcgctg gcgccggaga acagggtgac ggcgctgccg ttctcggtga
     1561 cggtgggcga caggccggtg gccgagaggg tcggcgcgtc gttggcgccc gtcaggtgga
     1621 cggtcagggt ggccgtgccg gtggccgggc tggcgttggc gtcggttgcc gtgacggtga
     1681 agacgtcggt gggcgtggca cctgctgtca atccattgat cgcaccggcg tccggaacat
     1741 aggcatagcg ccccgcgtcg gcgccggtgc tgaccaggta cagggtgccg taggtgccgc
     1801 tgcgggccac gtcgtaggtg atgccgccat gggtgaagcc ggcgttggtg ctggtgggcg
     1861 actggatgcc gaacgccgcg atgccgtctg cgtccgtggc gctgagggtg ccggtctgga
     1921 ccgcgaaggt gtccgtcgcg ccggtgtcgg ctagattgat gtcggcgggc ggtgtgatgg
     1981 tcggcgccgg gttgaggccg ctgaccagga cgccattgac ggtgtccgcg atgttcagac
     2041 tgctgtcagc gccgcgttcc cagttgtcgg cggcggccag attgtaggtg acgccgccca
     2101 cctgggtgcc attggcggtg agcaggctat ccacggcagt cttgtcggtg ctgcccaggt
     2161 tgagggtgaa ggatgtgtcg ctggtgatgt ccacatcgct ggtgctggcg gtcagggtgt
     2221 aggtcgctcc gcccgcgccg gtcagggtga ggcgcgtgac gtcgatgtcg ttggcgctgc
     2281 cggcgtggtg ggtgaggccg ctgccggtca cggtgagcac gccggtgccc gcgtcgtagg
     2341 tggcgctggt gatggcgggc agggccacgt tggagaccgt gatcgcgttg ccggtggtgt
     2401 cggcaatgtt ggccgacggc gtggctccgg cggcccagtt gtcggcggcc gccaggttgt
     2461 agctgctgcc gtcgctggcg ctggtgccgt tgcggttgag gatctggttg atccggctgc
     2521 ggtcggtggc cgacagggtg aggacgaact gggtggcgga ggtgcgctcc acgtcagccg
     2581 tgtccgtcag cgtgtatgtc gcgctgcctt cgccgccgag ggtgaagagc gctgcgacga
     2641 tgtcggcgcc cgatgcgttg gcgggaatgt tgtcgccagt gacgttcagc acgccggtgg
     2701 ctgcgtcata ggtggcagag gtgatgctcg gggcggtgga caggacccgg gcctcaatgt
     2761 cgctgccggt ggtcttgccc gaggtggccg aatcgaccca ggccgtggcg aaggcgccgt
     2821 tgtccagcgg cgtgacgacc ggagtgctct ggtcgccgta gcggtattcg ttgacctgga
     2881 actcctgggc gtcgacggcg ctaccggcgg cgtcgaaacg gcgtccctgg atggatgtgc
     2941 cgctgccgtc caggccgtcc gactgccaca cgacgacgaa gcctccgccg ccgagggcag
     3001 agacccgggc ggcgctcatg gccgttccct ggctgtcggc ctgcaggatg gaggaagtgg
     3061 gcgtgcccgc gttgctgaag gtctgggcat agacgtggct gccatccccc ttgctccagg
     3121 ccaccacgaa gccaccgccg tcgagggtgg ccatggagac gccggtgccg ttgtcgccga
     3181 cgggaccccc gctcacaagg gcgccgccga ccgtgaggtt gaagccggcc cagacgttgc
     3241 cgtcgatgct ggcggtcacc acgtcaccat tggcgagaac ggccgtgacg ggggcgaagc
     3301 ccgcggttac gccggagagg tcgacgatcg cgccgttcgg tgtgccgctg tcgctgagga
     3361 cggcagtctt gaccgtcgtg tagttgccaa ggttccaggt gacgctgaac tggctcccgt
     3421 cggcgctcgc ggcgacgccg atgggatcgt agccgtccca cccgccggtc tgattgccac
     3481 cgccgtcgac agcataggct tcgatcagtc cgttggacga ggcgctgccg gtggcgccgt
     3541 cgatgatctt gtaacggatg tcgtaggtcg tggcgtcacc atagacgacg gcgaacttgc
     3601 cgccggccag cgcggtgatc gaaggttgag ttgccgaact gcccgcgtcg atggtgatcg
     3661 gtgcgccctg ggcggttccg gcgctgtcga acacgcggga atagatgccg ccgttgctga
     3721 tatcggccca ggtgacgacg aaattgccgt tgctcagggt cgtgatcgcc ggttcggact
     3781 ggttgaccgt gtcgccggca agggggttga cggcgatgtc gttcccgaaa ttcagatcgt
     3841 gggacggacc cttgatggtg aaggtgtagg tcttggtgac gccctggccg ccaatgggcg
     3901 cgccggtcac gtcggtctgc agggcatcgt agaaatgcac cgtgacgctg gcggtttgtc
     3961 cttcgctcgg caggttggag gtggacgcga gtaccagatg ggcggtgttg cccgtggccg
     4021 cattgaagct gagcccgaac agtccggaga cggtctggct gtcgatggcc cagtagccgc
     4081 cgagggaggt gccggtatcc gtcggggtga gggtggcgat gtcggagccg aggggcgtgt
     4141 cgctcaccag cagaccgctg gaattgttgc tggcgatgtc ggtgggggcg gtgtcgatac
     4201 gcaccttgta gccgccaccg ttatagaagc ccgagttggc ggcggtggtg gtgttggtgg
     4261 catccagaca ggtgacggcg ccgaaatcga cctcgctcac gctgccgccg ctggcgtgga
     4321 agcgcaccag cgcattgggt ggcgcgaagg cgctccagtg gttcgcgaga attgtccacg
     4381 acgacccgtt gttggtgctg tattcgaagt agccggggcc gcccgagttg atctcgtagt
     4441 agccgaaata ctggttcgag ggcagcttga ggacggtgga cgtggtgccg ttgggacgga
     4501 tgtagaaggt gtcggtgggc aagctgcgga cctgcccgcc cgagacgacc gtcgcggggg
     4561 cgccccactg ccacacgtca ggattggcga gcaggccgtc atactgggcg aactggagac
     4621 tgcgggtctc gatgtggtta acagtggatt cgagcaccca gtcacccccg agacgggtgg
     4681 cgccggtggc gtcgtcggac gcggccacgt cggcgcgggt ggcccgcgca agggactgga
     4741 tcagatcggt tccggccgtg gtggcggcga cattgcagcc gtagagcagg atgtcgccgt
     4801 gggccttgag ggcgccgccg atggtgccca gggtgtcctg gtatgcgccg aaggtgtcgc
     4861 cgtccagggt ggcggtgccc agcgtgaggg cgccgctgct gccgtgtccg aggatgtgga
     4921 tcgcgtcgta gtcgtggtgg ctggcagccc attgggcgat ctgttcaagg ccgttgtgcg
     4981 tgccatcgac cagctggatc tcgatcccct gcgggatgcc gctcacgagg ctttgataat
     5041 cgacgacctg gctgtcgatg aaggccacct cgcgccgccc gccgttgagc gccgggtcca
     5101 ccgcgcggat cagggttgga ccgtcggcag ggctggcctt gtccgattcg cgggccgggg
     5161 tcgcggcctg ggccgcctgg gcggcaagcg cctcggcggc agcggcggct tcggcggcag
     5221 tggaatcgtg ggcgtgactg tccaccgtgg cgaccgcagc gccgtcgaac atgaagcgct
     5281 gctccagggc cagcggcctc agcgcgctgc gcagggtccg tgagccggga cggctgctgc
     5341 acgtcgggtc gccagacgca gaaggcgcaa cgcattctgc ggccgcgacg acactgctcg
     5401 aagcgggctt gccctggctt cgaacggttt cggcaacggc cacgaagctg cggctcactt
     5461 cgttccagat ggagcggtag atgcgattca tggcgacctc gtgggggcga ttacggattg
     5521 gctggcggcg tcatctggac cttgccgctc atgcctgcga tcagttcggg gaaacggccg
     5581 tcgatgacgg cgttcagctt gacggactgg ctgacggcat cgacgcgggc gccgatccgc
     5641 tggaccttgg ccgggtagct gcggccggtt tcgtcgatgc gcacctgaaa ggcggtgccg
     5701 gtccggaccc acgccagcca gcgggacggg acgatgaatt ccagttcgag ggcgctgtcg
     5761 tcgaggatct ccagcaacgc ctgacccggc tgcacgtact gctgttcgcg gaccttctgt
     5821 tcggcgatcc gtccggcgaa cggcgcggcg atggcgcatt tgccgaccag ggtggcattg
     5881 gcggagacct cggcgcgggc cttgagcact tcggcttcgc tggtttccag ctcgaccttt
     5941 ccgaccgagt tcagatccgc caggcgtctg ttggcctgcc aggtcttgtc ggccccggcg
     6001 agggcggccc gggccttggc gagctgggcc tgctgcaggg agcagtcgaa ttgcacgagg
     6061 gactggcctg ccttgaaggc accgccctcg ggaacgggca ggtggctcac cttggcgccg
     6121 atctcggccg cgagcgtggt gtagcgtcgg ggcgccagct gggcccggat ctcgcggttc
     6181 tcgagggcgc ttccaggatt cgaggcaact ggcgcgggcg ccgctgcgga tcgtggcggc
     6241 ggggtctggg accaggccgg cgtgacgagc agggcgttga tgatcagggc gatggaccgg
     6301 aggcgcatga tcacctcacg gagcgtcgcg gccggtcgcg ctcccgccgg cgtccggcag
     6361 ggccgcctgc tgccaggcgt ccagcgcctt gcgcacggaa tcggtgagtt cgggcagggg
     6421 acgctccgcg ccggcgtcga ccaccggctc caggcccagg gtggcctgca gcttggacgc
     6481 ggcggactgg gcgttggaca gggcctgata gcgccgcaac tgggcgagga cgtaggacgt
     6541 gctctgggaa acgcgatcca gccggctctg cttttccgcg tcgctctggt tcctggcgtg
     6601 cccggccatg cgcgcctcga tgtcggcaat ctcatcggcc cgctcgaact gatggatggc
     6661 gttggcgtac tgcaggcggg cgacgtgcaa ctgggtgagc accgccatct gggtgaccag
     6721 gcgcttctgg tcggccaggg ccacgccggc ttcgctcagg cgcatctggg cgggggcgct
     6781 gaagacgttg aagagattga aggagagctg gacaccggct tcgttccagg tctggttgat
     6841 caggtagctg tcggtgctgt gcttggtacc gtagttgaag gacaggccgg gaaacagctt
     6901 gagcagggtc cggcgggtct cctggcgggc gatccggctg ttcaggatgc cttccctgag
     6961 gtcggcgttg cgcatcagcg cctgctcttc gaggcgggcg acggggaggt cgagccaggc
     7021 cttctgcagg accgcgctgg ggggcgccac cgtgatctcc gtcgacatgg gcagattgac
     7081 gagggcggcc agttcggtgc gggccgtgga cagttcctgc tggaccgtct ccagcatgcg
     7141 gatgttctca agcacggtgc gctggtagcg caggggttcc atcgggttgc gcaggccttc
     7201 cttctccgcc ttgcgggcat cccccagcgc cgcctccgcc tcgcggatgc cttgtccgac
     7261 ggcctcatgg agggattggg ccgcgaccag acgccagtag gcagtgcgca cgtcctggac
     7321 caggagctgc agggccttgc ggcggcgttc ctgggcgatc agcaggcgat cggcgttctg
     7381 cttggcggcg taatagctct gcccgaagtc cagcaggctc catgacaggg tcagatccgt
     7441 ggtggtcgcc gtgcggccgg tggagatgaa gggattggcc agggacgggg caccggtcac
     7501 tgaatccgtg ctgtgggaga tcagatcgtt gttgcggtcg cggtagccgg ccgaggcgac
     7561 gagtctgggc agcagatcgt aggtgctggc gtcgaaggtg ccctgggcca gagcctcttc
     7621 catgatgcgg tagcgtcgtt cggcgttgtg cttgatggcg cgggcgatgg cctcttcaag
     7681 gctgaggacg ccggtgagtg gctccacgcc ctgcatcaat cgggcccggt cggcatccac
     7741 cgtgctcacg atctcggcgg gctgcagggg ttcgggcttc atcgaggcgc agccggagag
     7801 gagcaggagg ctgatggcga gggcagaaag cgtgagtggg cgcgtggtcg agcgcaagtt
     7861 cgtgcgatgc atggtggacg ttcctctccc cgtgaattga agcgttttag tttggcggtc
     7921 tccggcgtgc ggaggaggcc ggcgtcattc gggggcgggc tcccggcgtc ttaccccgcc
     7981 ggcgggacgg ggcgacagga cctggagcag aatcccttgc aggcactcgg cgacagacct
     8041 cgtacttatc gaccgaatga cgatgagctt taaggctgcg tggaaaaaat gatctgcgcc
     8101 tcatccggca cccggcccct cccgcagcgc ggccgcggcg accgtgggca tcatgacgag
     8161 cgtatttgcc tggccacgcc gggtgggcgc aacgtgcgcc gtcactcaga tatcgtcggg
     8221 tgggcgtgcg gcgggggcgt tcggtgcgcg gaaatgaaaa ggcccggcgg gtgccgggcc
     8281 tggaaggcgg agtctggtcg ggtgccgacc gacccgaacc ggggtgcgtg ggttaccagt
     8341 gggtttcccg gtcgggcgtc gcagtgatct tgtggatggt caggtccgcg ccttcccatt
     8401 cttcttcctg atcgagtcgg atgccgatca gggccttgag gccgccatag acgacggtgc
     8461 cacccgcaag ggcgacgacg atgccggcga gggtgccgat gatctgcgcc gccaggctca
     8521 cgccaccgat gccccccagg gcctggctgc cgaagatgcc ggccgcaacg cctccccagg
     8581 cgccgcacag gccatgcagc ggccagacgc cgagcacgtc gtcgatcttc cagcggttct
     8641 gggtgatggt aaagagctgc acgaacagga cgcccgccac cgcgccggtg acgagcgcac
     8701 cgaccggatg catcaggtcc gagccggcgc acacggcgac gagtccggcc agggggccgt
     8761 tgtggatgaa gccggggtca ttcttgccaa gcaccatggc ggccatcgtg ccgccgacca
     8821 tggccatcag ggagttgagg gcgaccaggc cgctgacctt gtccatggtc tgcgccgaca
     8881 tcacgttgaa gccgaaccag cccacggtca ggatccacgc cccgagcgcg agaaaaggga
     8941 tgctggaggg cgggtgcgcc gagatgttgc cgtccttggt atagcggcca tggcgggctc
     9001 caaggaggag taccgccggc agcgcgatcc agccgcccac tgcgtgcacc accaccgatc
     9061 cggcgaagtc atggaacttg gcgccgaaag cggcttccag ccagggctgg agaccgaggt
     9121 tgtcgttcca ggcgatgcct tcgaagaagg gataaacgaa gcccaccagc aggaaggtgg
     9181 cgacgctctg gggatggaaa cgggcgcgtt cggcgatgcc gccggagatg atcgccggaa
     9241 tggccgcggc gaaggtcagc aggaagaaga acttgaccat ctcatagcca ctcttctggg
     9301 ccagaacttc ggcgctgctg aagaagttga cgccgtaggc cagggtgtag ccgatgaaaa
     9361 agtaggcaag ggcggagacg ccgaagtcgg tgaggatctt caccagggcg ttgacctgat
     9421 tcttcttgcg gacggtgcca agttcgagaa aggcgaagcc ggcgtgcatg gcaagcacca
     9481 tgatggcgcc tagcaggacg aagactacgt tgctggtggt ggtgatctgc tccattttgg
     9541 gctccgaaaa tcgatggagg caccaagcaa gtgccacgcc agatctatgc tggtgcaagt
     9601 agatgaatcg tcaggtcttt tattccgacg atttgcaaaa aaagcactgt tatggtgctt
     9661 cggcgcgatg aaagcaccaa aataatgcat tgcgtttggg gtggtgcacc gatgtcgccc
     9721 cgcctgcagg cgcgggagcc cgaatggcca ccgctcgaac cgtgttttca gcccggtcgg
     9781 ggccggaacc acagggctcg ccgaccgtac agttgaagcg attcgatgtg tgcaggacct
     9841 ggggcccgct gcggcagcca taccgctccg gcgtctggtc cggggcgcgc gtgaaccatc
     9901 cgccggcgtc ggaccgtggg gaagcaaccg tcccgcgcga gcgtgtccgg aagctcatgc
     9961 agggcgtgca cgatgcctgt gtcgggctgc ctgaaggtgc cgtcggggtg aatgcggttg
    10021 aggacgcggt cgcgaaaggt gtcggactgg acgtgggtcc gtgcggcgtc aagcgcggtc
    10081 cggaacccct acgtcagggc ggaccaggtg gcacggtgat catcggggcg ctccgcacgg
    10141 tgcgcacgcc gggcgcgggc ggcgagacgg ccatcgtccg tgctcaggtc ggcgtccacg
    10201 atggattgat gcacgcgccg attgtgcaga ttcacctggt cctcggggcc atccacgatc
    10261 tggctgtgac tggattcgcg gatgagggcc tgagaggtga tccggaatcc gggaaggccg
    10321 gtgttgcccg gaccaccggc gcgcggtgag gtgccgtccg cagtggtggc gtgtggcacg
    10381 cggtgttcgc gggtgcgcgc gtggatgagt tgtccgaggg gcatgtgggc gaatgccctg
    10441 gccctcgggg tcgtgctggc gtccggggca tctggggatc ccacggacgt cgccagggtg
    10501 agcagcattt tccgacgcgt gccgtgcgct tgcaggcgga tccggcattc gtcgagtcga
    10561 tgggaaatgt cgtgaagggg ctgctgtcgt ggttcggggg gcggggctgc gtcatggacg
    10621 gcttccgggc gggaatggag gtccggtccg tgcgtgcttg actttgatca aagtaaacgt
    10681 atgattcaaa caaatgtttt aatctttggt ttgaatcttt aggccgagtg atcttctgcg
    10741 ccacgtgacc aacgcgaccc tgattctccg actgccatga aagacaacgt cagcaaaaat
    10801 ggttccgaca cccgcgagcg catcctcgat gccgccgagg tgctgttcat ggagcacggt
    10861 ttcgatggca cctccatgcg catgatcact gttcaggctg cggtcaatct ggccgccgtc
    10921 aattatcact ttggctcgaa ggagttgctg atccaggacg tcttccgcag gcgcctgacc
    10981 gatctcaaca aggcccgcct ggaggcgctg gacgcgttgg agcgccaggc cggcgacgct
    11041 ccggtaaagc ccagccgcat cgtggatgcc ttttttggca cggccctgcg catggcggcc
    11101 gatgtggacg gcggcgggca caccttcatg cgcctgcttg ggcggaccta caccgagccg
    11161 aacgagttcg tccgcaagtt cctcgccgag gaatacgcgg agtgtgtgga gcgcttcctg
    11221 gccgccctgt accgggcgct gccggaggtg gaccgccacg agatcctgtg gcgcttccat
    11281 ttcatgatgg gcgccatgtc ctacgccatc gccggcaccg atgccttgca actggtgacc
    11341 ggaaaattcg acgacgagaa cccgtcgcgc cttgcgccga ggctgatgag cttcctgctg
    11401 ggtggcctgc gggcgccgct ggcggacttc ggcccgcaac cggacgcctg agccgggtca
    11461 gttacgacat tcgacacgac aggcgcacaa gcgccgggaa aaaggagaca gcaatgtggt
    11521 ggttcctggt tttcatccct cccttggtgg tcgcgctcgc ctacgggcga gcgccatttt
    11581 ttgcctgggt gggggccatg ctggcctggc tggcggcctg gacggcattc ggcggtttgc
    11641 cgccgggcgc cgcgctgggt gttggtgccg tggtggcggt ggcgggcgcg ctgttcctgg
    11701 tgccggcgct gcgccggggt ctggtgacgg cgccgatctt tcgcgccttc cgcgccgcgc
    11761 tgccgaccat gtcccagacg gaacgggacg cgctggaggc ggggacggtc tggtgggaag
    11821 gtgaattgtt cgccggacgg ccggactggc gcacgctgct ggcgtatccg gtgccgcggc
    11881 tcacggatga ggaacagcgc ttcctggaca acgagactgc cgagctgtgc cgtctgacgc
    11941 aggactggga ctgcacccag cgccaggaca tgcctgccga ggtgtggcag tacatcaagg
    12001 acaagggctt cctgggcatg atcatcccgc gggaatacgg cggcaagggc ttttccgcct
    12061 ttgcccattc ccaggtggtg accaagttgt ccacgcgctc ttcggcaccg gcggtcacgg
    12121 tgatggtgcc caattccctg gggccggcag agctgctgct gcattacggc acgccggagc
    12181 agaaagccca ttacctgccg cgcctggcca gagggcagga gattcccgcc ttcgccctga
    12241 ccagcccgtg ggcggggtcg gacgcggcgt cgataccgga tgccggcgtc gtctgccgcg
    12301 gcacctggca gggtcgcgag gtgctgggca tgcgggtgag cttcgacaag cgctacatca
    12361 ccctggcgcc cgtctgcacg gtgttcggtc tggccttccg gctcttcgac ccggaccgtc
    12421 tgctgggcga cgtggaggat ctgggcatca cctgcgcgct ggtgccccac gaccaccccg
    12481 gcgtgaagat cggccgccgt catctgcccc tcaacgcggt gtggatgaac gggccgatcc
    12541 agggccagga cgtgttcatg cccctcgact tcatcatcgg cgggccgacg atggccgggc
    12601 agggctggcg catgctgatg gagtgcctgg cggccgggcg ttccatctcg ctgcccggct
    12661 ccaacaccgg catgcagaag atgacggccc gcgccgtggg ggcctacgcc cgcgtgcgtt
    12721 accagttcaa gaccgccatc ggccgcttcg agggcgtcga ggaggcgctc acccgtatcg
    12781 gcgccaacac ctacctgtgc gacgccgcgc gggtgatgac ggccggcgcc atcgacctgg
    12841 gcgagaagcc ctcggtggtg tccgccatcg tcaagtacca cgtcaccgag cgggcgcgtc
    12901 agaccgtcaa tgacggcatg gacgtgatcg gcggcaaggg catctgcctg ggtccgcaga
    12961 actttcttgg gcgcgcctac cagcagatcc cggtgggcat cacggtggag ggcgcgaaca
    13021 tcctgacgcg cagcctgatc ctcttcggcc agggcgcgat tcgctgccat ccctacgtgc
    13081 tcaaggagat gaatgccgcg cgggaaggcg atctcgccgc cttcgaccac gccttctggc
    13141 gccatatcgg ctacacgctc agcaatgcgg cgcgggccct ggtgaccggc ctgaccggct
    13201 cccatttcgt ctccgtgcct gacggcgtgg cgccggagac ccgccgctat taccagcagc
    13261 tgagccgctt ctccgccgcc ttcgccttcc tgtcggacat ctccatgggc accctgggcg
    13321 gcgcgctgaa acgcaaggag aagctgtcgg cgcgcctcgg cgatatcctc tcgctcatgt
    13381 atctggcgtc tgccaccctc aagcgttacg aggccgacgg ccggcccacc gcggacgcgc
    13441 cgctgatgca ctgggcgatc tgggactgca tgttcaaggc ccagaatgcc tttgaaggcg
    13501 ccattgccaa ctttcccaac cgcttcttcg cgatgctgct gcgccggctg gtggtctttc
    13561 ccctcgggcg gccctacgtg gtgccttccg accggctcgg gcacgaggtg gcgcggctcc
    13621 tcatcgagcc atcgccgacc cgcaaccggc tcacgtccga tgtctatctg cctaccgacg
    13681 tggaggagcc cgtcggcgcc ctggaggccg cgctgctggc caccgtcgac gccgagcccg
    13741 tggaggcccg tgtccgccgc gcccagcggg atggccagtt caggccgacc gtgtcggtgg
    13801 gtgggggtgt cgacgccgtc tacgatcagg ccctggccgc aggcgtcatc accgcggccg
    13861 agcacgccct gatcaagcgc cgcggcgtcc tgcgcgacaa ggtgatccgt gtggacgacc
    13921 atccctacga cttcggtctg cgcgacgccc tggccacggt ggatgtgccc gcgcgtcagg
    13981 cggcctgagc ccggatgccg ttcatgacga cacctgtcta catcatcgac ggggcccgca
    14041 cgcccttcct caaggcccgt aacgcgccgg ggccgtttgc agcgtcggat ctggccacgg
    14101 cggccggctc cgccctgctg atgcgccagc ccttcgcgcc ggaccagctc gacgaggtga
    14161 tcctcggctg cgccagccct tcgccggacg aggtgaatat cggccgagtg gtggccctgc
    14221 gcatgggctg cggcgtgaag gtgcccggct ggacggtgat gcgcaactgc gcctcgggta
    14281 tgcaggcgct ggattcggcc atcgccaacc tccagaccgg gcgctcgcag ctcgtgctgg
    14341 cgggcggcgt ggatgccctg tcgcgggcgc cgctgctgtt ttccgacgcc atggtgcgct
    14401 ggctgtccgg ctggtacgcg gcgaagaagt tcggccagaa gctggcgcac atgcgccact
    14461 tccgccttgg ccatctggcg cccgtgatcg gcatcatgaa gggcctgacc gatccggtcg
    14521 tgggccagct catggggcag accgccgaga acctggcctg gcgcttcggc atcacgcggg
    14581 acgacatgga cgccttcgcg gtggagagcc accgccgcgt cgccgccgcc caggatgccg
    14641 gccattttgc cgagatcgtg cccctcatcg accgggacgg cacgctttac acggccgacg
    14701 atggcctgcg ccgggatgcg tccacctcgg gtctcgccaa gctcaagccc ttcttcgaca
    14761 agcactacgg tcgcgttacg gcgggcaaca gcagtcagat caccgacggt gccacctggc
    14821 tgctgctggc atcggaagcg gcagtggtgc gccacggact gacgcccctg gggcgtatcg
    14881 cggacagcca gtgggccggc cttgcgccgg agcagatggg tctcggcccg gtccatgccg
    14941 ccacgcccat tctgcagcgc cacgggctgg ggctgcacga cgtggatctt tgggaaatca
    15001 acgaagcctt cgctgcccag gtgatcgcct gccggcgcgc ctggcaggac acgggctatt
    15061 gccgcgagca actgggcctg gacggcgccc tcggcgcgct ggatgtggcg cgcctcaacg
    15121 tggatggcgg cgccgtggcg ctcgggcacc cggtcggggc gagcggtgcg cgcatcgtgc
    15181 tgcatctgct ccacagcctg cgtcgcacgg gcggcaggcg cggcgtggcc gccatctgca
    15241 tcggcggcgg gcagggcggg gcgatgctgg tggaggcact gtgatggatc tgaaacactg
    15301 gaccctggaa cgggacgccg acggcctggc ctgggcgacc ctgaacgtgg cggacgccgc
    15361 caccaacacc ctgggccgcg ccgttctcga cgaactcggt gcgctgctgg atgatctgga
    15421 tcgccagccg ccgacaggcc tggcgatccg ctcgggcaag cccgcgggct tcattgccgg
    15481 ggcgaacatt gaggaattca cccgcatcga cagtgccgag gcagcgcgga cgctggtgaa
    15541 ccgcggctgg gctctgttca accggctggc cgcggtgacc tacccgaccc tcgcgctgat
    15601 ccgcggccac tgcatgggcg gcggtctgga gctggcgctc gcctgccgct accgtctgac
    15661 cgtagacgaa cccggcacgc ggctgtccct gcccgaggtg atgctgggca tctatccggc
    15721 ctggggcggc atgctgcgtc tgccgcgcct gatcggcccg gcggcggcgc tggatctgat
    15781 gctcaccggc aagagtgtgg atgcgctcaa ggcgaaacgc ctgggactgg cggacgactg
    15841 cgtgccgccg cgggtgatgg acagcgcggc gcgcgtcgtc ctgctgtccg ggcgttcgcc
    15901 acgccgcctg cccttcgggc aaaggctgct gaacgggccg ctgaagggcg tcgtggccgc
    15961 ccgggcgcgt acgacggtgg cggccaaggc gcgccccgag cactacccgg cgccctacgc
    16021 gatcatcgac ctgtgggcga accatggcgg aaatgccttg gcggcgccgg cggcttcgcc
    16081 gtcgtcgctg gaggcgatct tccgttcgcc caccgcgaag aacctgatcc gcgtgttctt
    16141 cctgcaggag cgcctgaagg gcttcggcaa ggacaccgat tttcgtccgc agcgggtgca
    16201 tgtggtgggc gcgggtgtga tgggcgggga catcgcggcc gaatgcgccc tgcgcggcat
    16261 ggtcgtcacc ctccaggacc agtccgcgga gcgcatcgcg ccggccatcg cccgcgccgc
    16321 gaagcgcttt gagcgcaagt tccggggcaa gcggcgggag gcccgcttcg ccctcgaccg
    16381 gctcatcccc gatccggcgg gccacggcgt ggcgcgggcg gacgtgatca tcgaggcgat
    16441 cttcgagaac ctggaggcca agcgcaccct gctggcggat ctcgaacgcc gggcgcggcc
    16501 cgacgccgtg ctcgcgtcca atacgtccag cctgcgcatc ggcgacatcg ccacggcgct
    16561 gcgggagccc gcgcggctgg tgggcattca ctttttcaac ccggtggcga tgatgcccct
    16621 ggtggaagtg gtgcgcaccg ggcacacggc cgacgcgccc ttgcgctgcg cggcggcctt
    16681 cgtgcgcgcc ctcgacaagc tgcccctgcc ggtgcgtgac gagccgggtt ttctggtcaa
    16741 tgcggtgctc ggtccataca tgtacgaggc gctgcgctgc gtggacgagg gcatcgcacc
    16801 ggccaccgtg gacgcggcac tgaccgcctt cggcatgccc atggggccca tcgagctggt
    16861 ggatacggtg gggctggaca tcgccgtggc ggcgggcacg gccctggctg gcgcggacgc
    16921 ggtgctgccc cggacgctgg ccgacaaggt ggcggccggg tggctgggac gcaagagcgg
    16981 gcagggtttt tacctgtggc agaacggaaa ggcgcaaaaa tctgcgccgg gccccctccc
    17041 ggacgggctc gcggagcgta tcgtgttgcc gttacgtcaa gccgtgcagg cctgcgtgga
    17101 caagggcgtg gtggccgacg ccgatctggc cgatgcgggc gtgatcttcg gaaccggttt
    17161 cgcgcccttc accggcggtc cgctgcacga tctggcgtcc cgcgcccgcc cctgaacatc
    17221 gtctggagct agccatgtcc gattccgatt cccgtctgcc cgaaggcaag attcccgtcc
    17281 tgcgggtcat gcccatgccg gcggacctca acccggccgg agacgtcttc ggcggatggg
    17341 tgatggccca ggtggacatc gccggggcga tccccgccaa gcggcgtgcc cgcggtcgcg
    17401 tggcgaccat ttcggtcaac gccttcctgt tcaaacagcc ggtctcggtc ggtgatctgg
    17461 tgagcttcta cgccaccatc gtcaaggtgg ggcgcacgtc catcacggtg gatgtggagg
    17521 tcttcgccga gcgcaatccg gaaaatccgg tggtggtcaa ggtgaccgag gccaccctga
    17581 cctacgtggc tgtggcgtcg agcggggtga agcgggaaat cccgccagag gaacccgcat
    17641 gaacgcaacg tgacacgaat ctgtttcagg accgcggcat gcagcggttc tagaatagcc
    17701 gggacatgcg ttccgcagtg ccggcctgct gccaaaacta aagcgtatcc acacgaggag
    17761 acacaaccat gaaaatgaag acccttgccc gcatgatgcc catcctggcc gtggcggccg
    17821 cgaccggaga ggcgtctgcc gccggctttc agctgctcga acagaacgcc agcgggatcg
    17881 gcaacgccta cgccggttca gcggcggtgg ccgagaacgc cagcaccatc tacttcaacc
    17941 cggcgggcat gacacagctg aaggaccggg aattctccgc cggcattgcc gtggtgcggc
    18001 cgagcttccg cttcgccaac gagaactcca gtgccggtgc cctgaccagc accggcaacg
    18061 gcggcgacgc gggctccacc ggcttcctgc ccaacctcta tgcctcctgg gcgctgaaca
    18121 aggatctgta cgtgggcctg ggcatcggtg cgcccttcgg cctcaagacc gaatatgaca
    18181 atccctggat cggcggcgcc cagtcggtgc gtttcgagat caagacggtg aacatcaacc
    18241 cgtccgtggc ctaccgcgtg aatgagaagg tctccatcgg cgccggcatc aactggcagc
    18301 gcatcgacgt ggactacacc cgcgtgctcg gcacgaacag cgcgcgcctc ggtgtcacgg
    18361 tgcctttccc gctgaatacg tccaccggca ccttcagcgc caatgattcc acctggggct
    18421 ggaacgccgg tgccctgttc accctgtccg agacgaccaa ggtgggcgtg tcctatcgct
    18481 cgaagatgcg ttacaccgtc aagggcgatc tgaccgtgca gggtccctac gcgcccctca
    18541 acgctgcccg cagcggcccg gccaatgcca cggtcgagct gccggacgtg ttcgtgctga
    18601 gcgtggtgca gcagctcgat ccgcgctgga ccatgatggg cgacatctcg tggaccggct
    18661 ggagcagcat cccccacgtg gacatcatca acagcaacag cggcaccacc gcccagcgcc
    18721 tcgacactga gttccgcgac acctggcgca tggccctggg cgccacctac aaggtgaacg
    18781 acgcgtggaa ggccaagttt ggcgtggcct acgatcagtc gccggtggaa gatccgcacc
    18841 gcctggtgtc gctgccggac agcaaccgca cctggtactc cgtgggcgga cagtggaaga
    18901 ccccggcggg ctcgctggtg gacttcggct ttgcctatct gcgggtgaac acctcgccca
    18961 tccataacga tcagagcagc gctctgcgcg gggtggtgga cgggcactac aactccaacg
    19021 ccttcatcgc cggcgcccag tactcgctgg ccttctgatc ccacttcggc cgggcgcccg
    19081 cgggccgtgt tgccgcgggc atgcctcgcc aatgcgccac accatgggtc gcctcgaaca
    19141 tcctcagccc gatgccaaga cccgcatcct cgatgcggcc gagcgtcttt tcatggagcg
    19201 cggctttgcc gccacgtcgg tgcgcatgat caccggcgcc gccggcgtgc cggtcgcgct
    19261 ggtgaattat cacttcgggt ccaagcaggg cctgatggag gcggtctatg cccgcgcgct
    19321 gggtaaccgg ggcagtcggg taagttatct cgaccgtctg gaggccgagg cagatgggcg
    19381 gccgattgcg gtgaacgtgc tggtggacgc cttcctgtcg tcggctctgc gcctcacccg
    19441 caaggactcc atccccggcg aggtgttcaa gcagctcatc gggcgggcct tctacgaacc
    19501 cggtccgggc acggaggcct tcttcccgga cgaataccgg gaggcagtgg accgttaccg
    19561 tcaggccttc atgcgggcct tgccccagct gtcccaggag gacgtggtgt ggcgcatgta
    19621 ctttctggtc ggcatcgtcg cctatgcgat ggcgggaaag gatgtgatgc ggatgaccga
    19681 gacctacgcg ctggcggatc agggacagcc cgaagccatt ctggcgcgcc tgcgaccctt
    19741 tgtggtggcc ggtttcctgg cgccgccagg ggctttgccg tccatcgccg aaatcccctg
    19801 aatttttgga tgttgcacct aagcgcatga ttgcgctaag atttgaacag ttgttcaaat
    19861 tgtgaaggcc gtgactcgac agccttcgcg gaacagcacg gaagacgggc ggacggtgat
    19921 ccgcccgcgt cgatgaatgt ccatccagga cacgattaaa aggaggaaca cgtgagcaga
    19981 ctgatcattc gcaaggtggc ggtgctgggc gccggcgtga tgggcgcgca gatcgccgcc
    20041 cattgcgcca acgccaatgt gccggtcatt ctctttgacc tgccggccaa ggaaggcgac
    20101 cccaacggga tcgccaagaa ggccgtcgat agtctcaaga agctggagcc cgcgcccctg
    20161 gcgacccgcg accgggccgc cgccatcgag ctgggcaact acggtgcgga tctggagaag
    20221 ctccgcgact gcgacctcat catcgaggcc atcgccgaga agatggaatg gaagctggac
    20281 ctctacgcca aggtggcgcc ctatgtgggc gaccaggcga tcttcgccac caacacctcc
    20341 ggcctgtcca tcaacaccct ggcggaaggc ttcccggagg ccgcccgcag ccgcttctgc
    20401 ggcatccact tcttcaaccc gccgcgttat atggcgctgg tggagctgat cgccacgcgc
    20461 agcacccacg ccgacaccct ggatgcgctg gaagcctggc tcaccacgcg cctgggcaag
    20521 agcatcgtcc gcgccaagga cacccccaac ttcgtcgcca accgggtcgg cgtgttctcc
    20581 atcctggcgg tcatgcacca caccgcgcgc ttcggcctgg gcttcgatga ggtggacgcc
    20641 ctgaccggtc cgctcatcgg ccgccccaag agcgccacct accgcactgc cgatgtggtc
    20701 gggctcgaca ccctggccca cgtggtcggc accatgcagg ccaccctgcc ggacgatccc
    20761 tggttcccgt attaccgcaa ccccgactgg cttgccgcgc tgatcgccaa gggcgccctc
    20821 ggccagaaga ccggcggcgg catcttccgc aaggtcggca aggccatcca tgtgctggat
    20881 ctggccgctc aggattaccg gcccagcgcc ggcacggtgg cgcccgaggt ggcggccatc
    20941 ctcaagaacc gcaacgtggc cgagcgcttc gcccagctgc gcgccagcga gcacgtgcag
    21001 gccaagttcc tgtgggcgat cttccgcgac gtcttccatt acgtggccgt ccatctgggc
    21061 gacatcgccg acaacgcccg cgacgtggat ttcgccatgc gctggggctt tggctggacc
    21121 accggcccct tcgagacctg gcaggccgcc ggctggcaga gcatcgccga ggcggtgaag
    21181 gctgacatcg ccgccggcgc gaccatggcc tccgcgccgc tgcccgcctg ggtgttcgac
    21241 ggtcgtacgg gcgtacatgg cccggccggc tcgtggagcg ctgcggatca gaccgacaag
    21301 gcccgctcca cgctggccgt ctatggccgc cagctctatc cggagcaggt ccttggcgaa
    21361 gcgccggccg atgcgggcgt caccctgtgg gagaacgagg gcgtacgcct gtggcacctg
    21421 ccggccaagg atgcacgtat cggcatcctg tccttcaagt ccaagatgca cgccatcggt
    21481 gacgaggtgc tggacggtgt gctggaagct gtcgcccgcg ccgagcgtga cctggatggc
    21541 gtggtgatct ggcacgaggc gcccttcgcc gtcggcgcca acctgcaaca ggtggcggaa
    21601 gcctgcaagg gcgggcagtt cgacgtgctg gagcgcaccg tcgccaagtt ccagcaggca
    21661 tccatgcgcc tcaagcacgc cctggtgccc gtcgtcgcgg cggtgcaggg catggccctg
    21721 ggcggcggtt gcgaattcct gatgcacgcc agccaccgtg tcctggccct ggagagctac
    21781 gtgggcctgg tggaggccgg tgtcggcctg atccccgccg gtggcggctg caaggaattt
    21841 gcgctgcgtg cggcggagct ggcatccagg gcggcggggg gcgacgtata ccccttcatc
    21901 cagaacatgt tcaacaccgt cgccatggcc accgtctcca agagcgccgt gcaggccgtc
    21961 gaactgggct tcgcgaagcc ctccgacgac atcgtgttca acccgcgcga actgctccac
    22021 gtggccctgc accgggcgcg ggccctggcg gaggcgggct accagccgcc gctggtgcgc
    22081 aaggacgtga ccgtggccgg ccgcaacggc atcgccacct gcgagatgat gctgatcaac
    22141 atgcaggcgg gcggcttcat ttccgaatgg gattaccggg tggcccgcgc cgccgccacc
    22201 gccctgtgcg gcggcgaggt ggagaccaac gccaaggtgt cggaacagtg gatcctcgac
    22261 gtggagcgcg cgctcttcct ggatctgctc aagtccgaaa agacccagca gcgcatcgta
    22321 cacatgctgg aaaccggcaa gcccctgcgc aactgacccg accccgaacg acacggggcg
    22381 cgccgtccac cggccgtcgc cccgtcccag gagacacgca atgagcaaac agattcagga
    22441 agcctacatc gtcgctgcca cccgtacccc ggtggccaag cgcaacggca tgttccgcaa
    22501 tgtccggccg gacgacatgc tggcccacgt cctgtccgcc gtggtcggca aggtgcccgg
    22561 cctggacgcc cgcgagatcg gcgacgtgat cgtcggctgc gccatgccgg aagcggagca
    22621 gggcatgaac gtcgcccgca tcggcgtgct gctcgccggt ctgcccgaca ccgtgccggg
    22681 catcaccatc aaccgcttct gctcctccgg cctgcaggcc gtggcggacg ccgccgcgcg
    22741 catccgcctg ggcgaggcgg acgtgatgat cgccgccggc accgagagca tgaccgtcat
    22801 gccccagatg atgggcaaca agatctccct caacccggcc gtcttcgcca aggatgaaaa
    22861 cgtggccatc gcctacggca tgggcctcac cgccgagaag gtggcgcagc agtgggagat
    22921 cagccgcgaa gcccaggaca gcttcgccgt ccagtccaat caccgggccg cggcggccat
    22981 cgccgccggc gccttccgcg acgagatcac cccctacacc gtgcgcaccc acgtgcccgg
    23041 cgcgggcggc gtggtgcgcg taagtgagcg cgtctgcgat accgacgaag gtccgcgtcc
    23101 cgatgcgtct gtggcggggc tcggcaagct gcgtccggtc ttcgcggccc gcggttcggt
    23161 gacggccggc aacagctccc agatgtctga cggcgccgcg gcggtgctgc tcatgtcgga
    23221 agcggccgtc aagcgctacg gcgtgacgcc gatcgcccgc ttcgtgagct attccgtggc
    23281 tggtgtgccg cccgagatca tgggcatcgg ccccatcgag gcgattcccc gcgccctcaa
    23341 gctggggggg gtggcccagt ccgacctgga ctggatcgaa ctgaacgaag ccttcgccgc
    23401 ccaggccctg gcggtcatga agcagctcga catggacccg gccagggtga atccccaggg
    23461 cggcgccatc gccctcggcc atcctctggg cgccaccggt gcgatccgca ccgccacgct
    23521 gatgagcgcc atgcagcgcg gccaggcccg ctacggcatg gtgtccatgt gcatcggcac
    23581 cggcatgggg gcggcgggaa tcttcgagcg ggtgtaagcc cggcggcccc gcgccgcccg
    23641 cagcaggacg cgcaggggcc gccttcctcc ggggaggcgg cccctgtgcc atccgccggc
    23701 cacagcgccg gcgagccata caatgatgag gagaccatca tgatgcccac cggcgacggc
    23761 ccctacgcct atccgctgct gatcaagcag ctcctgcgca tgcccctggt gcacgccccg
    23821 gaccaggaaa tcgtttatcg cgaccaggtg cgccacagct accgggagct ggccgcacgc
    23881 atcggtcgcc tgggcagcgc cctcagccgg ctcggagtgg cccgcggcga caccgtggcg
    23941 gtggccgact gggattccca tcgctacctg gaagcctatt tcgcggtgcc catgctgggg
    24001 gcgacgctga tgacggtgaa tgtgcgtctg tcgcccgagc agatcgccta cacgctggat
    24061 cactgcgagg cggagaccct gctgctcaat gtggacttcc tgcccctgct ggaaggcatc
    24121 cgcgggcgcc tgcccaagct gaagcgcctg atcctgctgg ccgacggcgt ctgcgatccg
    24181 gtggccgccg gcgcgctggc cgaatacgag gcgctcctcg cgacggggcg cccggaccac
    24241 gcgtttccgg atttcgacga gcacacccgc gccaccacct tctacaccac gggcaccacg
    24301 ggcctgccca aaggcgtcta cttcagccac cgccagctcg tgctgcacac cctcgccggg
    24361 ctggccgggc tggcctctgc gccaggcacg cagcgtttcg accgtggcga tgtgtatatg
    24421 cccctcacgc ccatgtttca cgtgcatgcg tggggcttgc cttacgtggc gacgctgctg
    24481 ggcgtgaagc aggtctatcc cggccgctat gtgccggacg agatcctggc gttgcgcgcg
    24541 cgggagggcg tgagcttctc ccattgcgtg cccaccatcc tgcagatgct gcttctcgca
    24601 ccgtcggcgc gggacatgga tctgtcgggc tggaagatgg tgatcggcgg ttccgccctg
    24661 accgccggac tggcgaccca ggccgtggcg cgtgggctgg acctgttcgc tggctacggc
    24721 atgtccgaga cctgcccgat cctcaccctg gcccagctca agccggagga actcgaattg
    24781 ccgcccgagc agaccgtgtc cgcccgctgc cgtaccggcc tgcctctgcc gctggtggat
    24841 ctgcgcgtgg tggatgccga cgggacagaa gtgccccatg acgaccacgc ccagggcgag
    24901 gtggtcgtgc gcgcgccctg gctcacccag ggctatacga agaacccgga ggccaccgcc
    24961 cagctgtggg cgggcggctg gctgcacacc caggacatcg ggcgcatctc ccccgccggc
    25021 tccctgcaga tcaccgaccg catcaaggac gtgatcaagt ccggcggtga atggatctcg
    25081 tcgctggagc tggaaaccct cgtttcgcgg catcccgccg tggccgaggt ggccgtgctg
    25141 ggcgtaccgg acgcccgctg gggcgaacgt cccctgcctg tggtggtgcg ccgggccggc
    25201 tgcgaggcgg tggatgcgga ggcgatcatc gcccacctgc gggcctgcgc cgaggccggg
    25261 cagatctccc gcttcgcggt gcctgaccgg gtggtcttcg tggaggcact ggagaagacc
    25321 agcgtcggca agctcaacaa gcgcctgatg cgcgagcgtt acgccgtggc cgatggtccg
    25381 cgttgacggg cgccgggcgc ttcccgcctg gcgcgtttcg cggtgacaat acgcgcacat
    25441 tccttctgga gcccgatcca tgacctcgcc cgtcacgctg gacctgcagg acggcatcgc
    25501 caccctgacc ctcaaccggc ctactgcgct caacgccctg tcggtggaca tgatgaaagc
    25561 cctggccgaa gccaccggcc agctgcaacg ccgcaccgat ctgcgggccg tggtggtgac
    25621 cggatccggc gagcacttca tggcaggcgg cgatctgaag gatttcgcga cgcacctgca
    25681 tctgtcggcc gaaagccgca tcgccacctt ccgggcgctg atcgagcagt acatcaatcc
    25741 cagcgtcgag gccctggcgg ccttgccggt gcccgtcatc gcccaggtgc ggggcgcctg
    25801 cgcgggcttc gggctctctc tggtgctcgg ctgtgacatg gcggtggccg ccggaaacgc
    25861 ttatttcacc acggcctacg ccagcattgc cctgtccggt gacggcggcg tgtcctggtt
    25921 tctgccgcgc atcctggggc ggcgcaaggc cttcgagctg ctcctgctgg cggaccgctt
    25981 cggtgccgat gaggccctgc gcctgggtgt ggtgaaccgc gtcgtgccac ccgaagagct
    26041 ggaggcggcc acggatgccc tcgtccagcg cttcgtgaag ggcccgcggg aagcctacgc
    26101 cgagatgaag cgcctgctgg tggcgtcgcc cgaacagtcg ctctcggcgc agcttcaact
    26161 ggaagcggag gccttcgccc gcagcagtgc ccgacccaat ttcgatgaag gtatccgcgc
    26221 cttcctcgaa aaacgcaaac ctggcttcaa cgcctgatca ggccgccgcc caggtctgtc
    26281 aggcgccggg cggcgagacc cagccattcg tgggcggcat agccgccggc gaaggcggca
    26341 ttcatgcccg gcaggaaatc caccacttca tccgccggcc cggcgccgtg gaagaaggcg
    26401 gtgggcgccg ggatggcatg catgccggcc gcttcaaatg cttccagcgc ccgccgcatg
    26461 tgggctgcgt gggtgacgag cagcacgcgt cgcacgcccg ccgcgcgcag cacgggcacg
    26521 ctgaaggccg cgttttcggc cgtgtcgagg gactggctct cgacccagcg cactgacacc
    26581 ctgaactcct cttccatcac ccgccgcatc aggatgccct cggcaatgcc cggggtgggg
    26641 ctgccgccgc tgaccagaac gggcagacgc gagtgacggg cgagcagggc gccatagcgc
    26701 aaacgctcaa gggtgagccg attgacggtc tgtccgccga attccggcgc gtaatggcgg
    26761 gtaccgcctc ccaggatcac gatcgcctcg gcggcaggca ggtctcgcac atcgagggcc
    26821 gcgtcccgtt caaggggcgt gacgagcaga ccgacggagg ccggcaggct cagggcaatg
    26881 ccggtcacca ccccgcacca ggccagcagg cggcccgtgc ggggctggcg gtggagcagc
    26941 agcaggccgg cgatgatcag cagaaagggc aggaagggcg gcaggagcag cgcggcggcg
    27001 atctttttca ggaagaacat ggcgaaaggc gaggcgggga gccgactcca gattgtcggg
    27061 ggcgggcgtg acggctatta tcgcagcgtc aatcccgccc tgcccattgg aggctcatca
    27121 tcgtcggaat cgtgaaggca tcgttcattc ccgtcgcctg ctccgggagg attccacgat
    27181 gatcgagatc ggtgtgcggg aaggcatctg gatcgccatc ggcgtcctga ccctttatct
    27241 ggccttcgtc ctgatccgtc tggcgctcca tcgcgcccgt cccctcgcag aagaacccca
    27301 gacactggtg gtcgatgcgg aagcgcctgc gccgcaaggt gtggcgcccg ccgctctaac
    27361 cccctccggc gaggatgtgg cgtcgatccg gacgcctgcc ccgaccgagg atgcggacgt
    27421 tggcgacatc gaaccgttgg atgattacgc cgacccccgc accggtcggg gtgagtcttc
    27481 ggctggcgaa cagcccgaca gcctgccaac cttcgacctg atcgagttct tccagctgga
    27541 agtccagcag cttcgacggg atgtggccca gttgcggggc gaacaggatc agctgcggcg
    27601 cgagatcgcg tcggtgcggc gtgatctgga cgaaatccag gtggccgacg gccgcagtca
    27661 tttcgtgcgg ccggtgtcac cccagcattc cgaagccatg gtgctggccg aacggggcat
    27721 gacggctgca cagattgcgg atcgctgcgg aatcgccgtg gccgaggccg aactggtgtg
    27781 tgcgctggcg cgtcgggagg tgcatgaatg accgatgaac ggaacaaccc gccggttcag
    27841 gacgaaaagg atgattcccg gcggcagttg tggacccgcg ctgcggtggc cgtcgggctc
    27901 atcggcctgc tcttgggcag tctgatcgtt ttcgacgagg cctcccgccc gccggaagca
    27961 gaggaggcca aggtgccggc caagcccatc ggtccggcac cggtggcggg ccgcgagagc
    28021 gagccgccgc cggacgtcgt gcgcgcagga ggcgaggcgg cggaccaggc gtcctcggcg
    28081 cctcccctgc ctgtcgctgc cgatcagggg gaaccgccca aggcagcccg caaggcggat
    28141 gatgctgcgg acgagacccg acctgtcgtc ggggcgggcg cccgtacggt actccctgaa
    28201 tcgccggcgg tggcctccag caggtcggtg acactgaagg aagacaccct tcgcgggggc
    28261 gtcgcgccgt tggcggctac accggcggcg gcgcctgccg gtgcaccgtc tgcgccggca
    28321 ggcgcgccgc aggcgcgtcg cgaaccggtg ggcggcggac tcgcgtcacc gccggggagc
    28381 gcgccggcgg ccgtcgctcc gcaggctgag gtccgtcctc ccgcggatgg ccgcgcagca
    28441 ccggcgcggg gctatgtggt gcaggtgggc ggcttcatgg agcccgcggc tgcagaggac
    28501 ctgcgggcgc gtctggcccg tggcggcgtg ccggtccagc tggagagccg ggtgatggtg
    28561 gggccatttg ccgaccggcg agaggcactg gctgcccagt cgcgcctgcg ggagcagggc
    28621 tacgcgccgg gcgtgatcca gccttcccgt tgatcctgcc gctgaggcgg tcggctcagg
    28681 ccgcctcgat gcgtagggcg tgaccgccgc ggcagttcag ttcgaggccc gacaggtttc
    28741 cggcatcggc gcggcgcccc cacagggcgc tgccgtcgcc caagctgcgg gcgatctcgt
    28801 agccgcgctg cttgagccag accggatcga catgctgggc gacgacggtg gattgttgcg
    28861 gtgcccgggc gtgggcgagg atgaggcgga aggttcccat ggcggtctcc acatcggggt
    28921 gacgcgcgca ctctagcgga cgaatgtgac gaatgatgga gccggcgccc tcagcccggc
    28981 gctgtctgca gcatggctgc gggacggaaa tcctggagca tgcgggtggc ggcttcagcg
    29041 ccgagcggct tggaaaagaa gtagccctgc atctcgtcac agccggcggt ttccagcacg
    29101 ctgacctggt ctccccgttc gacgccttcg gccgaaacct gcacgtcaaa accccgggcg
    29161 atgccggtga tggcgttgat cacgggatgg ctgccctgcc cgttgatgtt ccgcacgaag
    29221 ctgcggtcga tcttgagacg ggtcacggga aagcggtgca gcaaggacag ggacgacaga
    29281 cccgtgccga aatcgtcgat ggacagaccg acgcccatgt cgcgcaaacc ctggaccttg
    29341 gccgcaagaa attcgtggtc ttccagcagg agattttcgg tgatctccag ttcgaggctg
    29401 tccggtggca ggttgtgctg ggcgagggcc ttgtagatgg cgtcgttcag gtccgcccgg
    29461 tcgaagtcgc gcagggacag gttggtggcc aggcgcagtt cgggatacag gcggcgccag
    29521 gcggacagct gggcacacgc ggtctccagc acccagcggc tgatgtcggg catgatgccg
    29581 acttcttccg ccagttgcag aaaacgggat ggtgcgacga ggccgtggtt acgatgccgc
    29641 cagcggatca gtgcctccat gccggtcacc tggttgcggg acaggctgac acggggctga
    29701 tagaacagtt cgagttcccg gttcgagatg gccagccgca attcggtctc gaggctgata
    29761 cgcgcccggt acgccgaatg catgccaggt tcgaagaatt cgtgggattc accgccattg
    29821 cgcttggcct ggaacatggc gatggccgcg tgctgggtga gggactctgc gtcgtcgccg
    29881 tcttcggggt agagggcgac gccgatactg cagttcaggc ggacctcgtc cttgcccaga
    29941 gagaagggct gctcgaaggc ctggtggatc ttgccggcaa tggcctgggc gttgtccttg
    30001 ccgcggacgt ccggcagcag gatcgtaaat tcgtcgccac cctgccgcga caaggtgtcg
    30061 ccccgccgca ggcagcgctg caggcggcgc gcgatggcgc acaggagcgt gtcgccttcg
    30121 ccctgaccga agcgctcatt gacgtccttg aagcggtcca cgtccacctg catgacggcg
    30181 atctgcccct tgcgacgctt ggcctgggcc acggccaggt cgagccggtc gaagaggagc
    30241 tcgcgtccgg gtagtcgggt gagtgggtcg cgctgcaggg gagggttgcc ggccagtgcc
    30301 gtcaggcgga gcggcggtgc gtggcgggcg acgccatagg tgcccagata ttcacccggc
    30361 gtcgtgccgt cctcgtcgtg gtagatgcca tgggcgttga gcacaaaggg caggggcccg
    30421 gcctcgccgt cctggtccgg accgaaggga ctgcgggtga gacggacttc cacattggcg
    30481 gcggcgcgct ggccggtgcg gcgctcgtgg aaggtctgac gggtccgctc cagatcctcg
    30541 ggatggacga tctccgagaa atgttggccg atcaggctgt tgcggcgata gcccagcagc
    30601 gattcgatgc gcggattgat gtagttgaag cgtccgtccg gggtggtggt gaagatcagg
    30661 tcgggggagc tttccaccag atagcggtgc atgtgctcgg acgattgcag gcgctgcagg
    30721 gtgaggcggt gactgcgctc ctgctgacgt ttgccgagga ccgactggac ggcacgacgc
    30781 aactggtcgg ggtcgtaggg cttgcgcagg aagtcctggg cgccgcggcg gagggcggca
    30841 atggccgctt ccatggaatc ctcgccgctg atgacgatca ccgattcgtt gcggtgatgg
    30901 ctcgacagcc agttcatgac ggcgagaccg gtggcgtcgg gcaggcggta gtcgaggatg
    30961 atgaggtcgt aactggccgc gccgaggcgc acgagcgttt cggcgacgga gccgcaatct
    31021 tcgatcaggc ggtcatcgcc ttgaagcagg gcgacgaccg aacgccgcag ggacggctgg
    31081 tcatcgacaa caagtatgcg gatcagagga tcgggtgcgc ctcggtttcc cgtctcgggc
    31141 gggaagaagg ggcgctcccc gggaatgacc atggatggac tccctgtgaa ttattgtttt
    31201 gcgccgcaat gatgcatcaa ttcagggggt gcccgccagc ggtatgaaga tctggaacac
    31261 tgtgccggag cccagctgac tgcggcacag gacggtcccc ccatgcgcgg tgatcagatc
    31321 ccgtacgacg ggcaggccga tcccgccttc cgtgcggccg gcgccgccgg gcaccggtgc
    31381 aaagagcgtc tcgatgcggc cggggggcag gcccggtccg ttgtcgacca cgcggatctc
    31441 caggcacgag gtgccgttgg cgttgatgcc ggtcccgacc gtgacgacga gcttgccgcc
    31501 ttccggaatc gcttcggccg cgttcttgaa cagattgacg aggacctgct tgagggcatc
    31561 cggagcgatg gctgcggacg gcagatcggg cgtggtgcgg acttcgaact ggatccctcg
    31621 ccgcgcgaac agggcgtcac cataaagggc acgcaggtcg tggagaattt cggtgacgcc
    31681 gctttgagcc gggccgtcgg ccgcaggctc gcggaaatcg ggggcgcgac ggacgaggtt
    31741 gccgatgcgg tcgagttcgt cgttgagcac gccgagctgt tcgcggatcg cagcatcctc
    31801 gatccgttcg tcgatgatgc ccaggtaggt cttgatgacg gtgagcgggt tgctggcctc
    31861 gtgggcgagg cgccgggcat gttctgcata gcgccgcgag atggtctgct ccaggtcgtc
    31921 gcgcagatgc tggctggcac gttcttcgga gaggcgcagg gtggccgaca ggacgagatc
    31981 gcggcgcagg gcgcgtgtgc tgtctggcgg gtcactgtcc gcgctggcaa aggcgaagac
    32041 ggcgacgccc gcgtcgtgat gattcttcca gggtacgcag agcatcccgt cgctgcccag
    32101 ccagcgggac acctgccaat cgatggcgct gcggccgggc gggcgttcgt cgcagcggta
    32161 acgggtctct gcattgctgc gagccgcttc ggcgatggca ctggtgtcat cggccagggt
    32221 gacgcacatc tggtcgaggg acggatgggt cgcgctggcg cagccgacca aagcaagtcc
    32281 gttatcgctg cggacgaaga gtgcgggtgc gtcgagcccg aggagcaggc gcgccgcccg
    32341 ggcgagacca ttgcgcgctt cttctgcgct ggcaaaggtg ccacgggcaa aggcattgag
    32401 tgcggcacgg gagatgcccg tggtgccggc ttcgggcgca ggtgccacat ccgggccgag
    32461 ggcccagggc agggcgagca tcgccggttc gatggcaacg ccgaaatcgc tcgctgcgtc
    32521 attgacctgc ggaccggttt cggcgagcag gctgaccagc acgctgccgt cgaggcctgc
    32581 caggtggctg gcgatatcga gggagagggc cgactgggtc tgacgatagc tggccagctc
    32641 ctcggcggcg gcgatgaggc ggaccagcag gtgagccgag cgcagctgca caggcgaggt
    32701 ctggtgcagc aggatggcgt cggcgaccag ggcccccaga ccgcactggt gggccagttc
    32761 ggccgaaatc gtgctgtggt cggcgccgta gcgttcccat tcggctgcac tcagggcctt
    32821 ctcgtcgctc agcgcagcac gcatggtcac gtagccttcc tgatgttcgg cgagccagag
    32881 ctggccgaca tcgtggagaa gcccggcgac gtacgcatct tcgggcgtcg ggtaggcggt
    32941 ctcgcgggcc aggtgaaagg ccagttcggc gaccagctgg ctgtgcaggg acaggttgcg
    33001 cagggagccg atggaggtcc gctgtccggg tgccggtacg gcgtggcgca ggaaccaggc
    33061 ctgcagcagg ccgggatcga gttcggcgat gcgccgctcg acagccttga gcggcgtgct
    33121 gcgctcgtcg cgaccgaggg gggccgttgt gaggatttcg agaaagagga tcggatcccg
    33181 gcagatccgc agggagatgt cttcactcga cgtcgtgcct tccgcgacca ccttgagcag
    33241 ggtggcggcg cattccggca gcgcaggcat ccgcgatacg gagaccgggc tgggaagccg
    33301 cagggaaagc gatctggtgt caggcatgaa ggcagtgata agggttggac ggttgccgcg
    33361 aaaggcttga tccaattcta accgtaggcc tgaggggacc ggctgtgaca aatgttcggt
    33421 cgttccggga cgttcgtgac ccagttcgca aacgaacgaa gagttcgcaa aggattgttc
    33481 ggccccccgt tgcagggccg aaatgaagcc ggtatcagcc gaactgcagg ctgtcggcga
    33541 agccgagcgc ttcgaggtgg gcacgattga tgacatcggc agagatgtgc agatccgccg
    33601 cgagttccgt caggtgtttg tcatcgaagc cttccagcgc acgggccagc ttgaggaagg
    33661 gggcgtagac accttcgccg ctcagcaggg cgtcggcgat gggttcaggc aggtgcatct
    33721 cctcaagcgc agagtgcagg ctggcgccga ggagcaggtg gagcatggag aaggcgccgg
    33781 tgatgaacag gttgtcgagc tggttcctgt cgaagaaggc ttcgccgatg cgttccatga
    33841 agcgaccgcg ggcgatggcc gtctgcatca gggcgggcgc agacggatcg cggctggccg
    33901 agacgaggag cacggacagc cacttgtgca gcttgtcgta gccgaggatg gtgacggcgt
    33961 gacggaagga ctggatctcg cacatcagcc cgaagccggc cgagttgatg taacgcagca
    34021 gtttgtagga cagcgccacg tcctgcttga gaacggtctc cacctccttc acttcggcat
    34081 tgcggcgcac cagattgagc aggcgcacga tctgagcgtg ggatggcgcc agcttgccgc
    34141 ctgcgttgag gcccttgagg aagaaccagc cggaggcgcc gtcatagccg ctgccgatgg
    34201 cggcgcggaa ggcggggaag tcctccagtc cccaggcaat gctgagtcct ggcggatcga
    34261 ggggcgccgg atgcttgtgg gcgtccatga tcacgaagcg ccagtccacg tcgggcggca
    34321 gcgtcacgtc cgggtgatac caggtgaggt tgaggctgac gccctgggcc cgtagctggg
    34381 gaatgagctg ctgggtcagg ggatgggcga tggcctgggt ggggatttcc accatggcgt
    34441 tggccggagc gatccactcc agcaggtcgg gcgtggggac cagccggtaa aggctgatga
    34501 agaccgggtg ggtggcgggc cacacgtctg ccacaccgtc gagggtctgc acgacttcgc
    34561 ccaccgattt ggcgtggacg accagccggt tggcggtgat cttgtactgc ttgttgatca
    34621 ccggttcgcg ggtgatcagg gcggtgtggc tcatgggcgt cctcgtggcg gtgcgggtgt
    34681 caggccccgc gggcgtcgtt cgagaagctg aaggcgtcgg cgaagaaggc gtcgggcggc
    34741 aggtcgcagc gcgccaccag atcgctgcgg gctgcgtcga tcatggcggg cgaaccacag
    34801 gcgtacacct catggccgga cagatcgggc agatcttcca tcagggcgtg gtgggccaga
    34861 ccgcgacgac cgtcccacgc ctcgccggcc gggacgtcgg agagtaccgg cacgaagcgg
    34921 aaacccggca ggcgggcttc ccaggcgcgg gcctcatcga actgatacag tccggcgcga
    34981 tcgcggctgc cccagtaaag ggtcatcggt cgtgtcgagg cgtcggcaat ggcctgctcg
    35041 acgatgctct tgatgggtgc gaagccggtg ccgccggcca gcagcacgac gggtttagcg
    35101 gcgccgggct gcaggccgaa gctgccgagg gggccctcaa aacgcaggat gtccttctct
    35161 ttgaggcgct cgaagacgtg ggtggtgaac tggccgccgg ggaccaggcg cacgtggatc
    35221 tccagatggt cggcgccgtc gagcggcgcg ttggcgatgg agaagctgcg ccgcttgccg
    35281 tcggccagga ggaagtcgat gtattgcccg gcgcggaact ggaatcgttc gctggccggg
    35341 agcttcagat ccagcacgat cacgtcgtcg cagaggcgct gcagcttctg cacgcggcag
    35401 ggcatcttct tgaccgggat ctcgccgacc agattgacct gacggacttc cagttcgaga
    35461 tcggtcaggg gcctggcgca acacatcagg gccatgccct gggcgcgctc ggacgctgtg
    35521 aggcttgatt ccgaatagcg cccgaggtcc acacgccctg agacaacctt gcccttgcag
    35581 acaccacagg caccgtcacg gcagctgtag ggcagcaaca agcctgccgc aagggcggac
    35641 tggaggaggg tttcatcggc agaggcggcg aactggtggg cgccgggctg gagcgtgacc
    35701 tggaacgaca tgatggatgg ccgtgagata atgaacggat gagtcgtgtg aaaacgaagc
    35761 gggttctgat tgttggcagc ggcgacgtgg cgcgccggct gattccgtgg ctggcccgcc
    35821 gctgtcgggt gtttgccgta atccggcgcc cggaagagcg ggaagcgctg cgggcgctgg
    35881 gcgccgtgcc gctggtggcg gatctggatg atcgggcgag ccttgatcgg ctcgccggcg
    35941 tggcggacgc cgtcctgcat tgcgcaccgc cgcccggaca gggttccgac gacccccgga
    36001 cccgcagcct gctggccgcg ctgtcgcgac gcgccagtct accacgtcgg ctcatataca
    36061 taagtacgac gggcgtttac gggaattgtg gcggtgcgtg gctggatgag acgcgtcctg
    36121 ttgcgccggc gtcgtcacgg ggcgtgcgca gggtcgccgc ggagcgccgc ctgcgcagct
    36181 tcgggacgcg cagcgggtgc ggggtgagca ttctgcgggc accgggtatc tatgcggttg
    36241 accgcctgcc cgtggagcgc atccggcggg gcgatccggt gctggcggcg gacgacgatg
    36301 tgttcaccaa ccatattcat gccgacgatc tggcccggct gctcggcttg agcctgttcc
    36361 gcggccgcgc aaaccgggtc tacaacgcct gcgacgacag ccagatgcgg atgggcgact
    36421 atttcgacgc cgtggccgac gccttcgcct tgccgcggcc tcagcgcctg ccccgggccg
    36481 agttgatcgc acaggtttcg cccctgactc tgtcgttcat gtccgaatcc cgacgcattc
    36541 gcaatgaccg catcaagcgg gaactgcgtg tccgtctgat ccatcccacc gtcgcagacg
    36601 ccctgaagcg tctggcctga gtcctgaccc cactattccg tttcctgagc cttcccatgc
    36661 tgatcgtcaa agcccttcac atcatcttcg tcacgagctg gtttgccggc ctgttctacc
    36721 tgccacgtct gttcgtcaat cacgccatgg tcaccgatcc ggcgaccatc gagcgtattg
    36781 cgctgatgga gcgcaagctc taccgcttca tgacgcctct gggcatcctg gccgtgctcc
    36841 tcggcatgtg gctgtggttc ggctacggct tcgtcggcgg ctggctgcac gccaagacgg
    36901 cgctggtcac cctgctgatc gtctatcacc tgtattgcgg ccgcctcctg cgggcctttg
    36961 ccgaggggcg caacacccgc agccacgtgt ggtaccgctt cttcaacgag attccggtcc
    37021 tggtgctgtt tgcggtggtc ttcctggtgg tcctcaaacc tttctgatcg ttctttccgg
    37081 taccgtctct tggacttctt tgctcccgta ccccgcggcc tggagccgct tcttgtcgac
    37141 gaactgcgca gtatcggcgc cgtcgatccc aaggcgctgc ccggcggcgt ccagttctcc
    37201 ggcgactggg cggtgtgcta tcgcgccaac ctggaaagcc gcctggccac ccgcgtgatg
    37261 ctgcaggtgg cctccgggcg ctaccgcagc gaggacgacg tctatcgcat tgcctacgcg
    37321 cccacctggg cccgctggtt tacggcggat cagaccctgc gggtcttcgt cacggcgcac
    37381 aagtcgccct tgcgcagcct ggaattcacc accctgcgca tcaaggatgc ggtctgcgat
    37441 catttccgca gcgtggccgg acgacggccc aacgtggaca ccaaggcgcc ggacgtgcgc
    37501 atccacgcct ttctgtcggc cgataccgtc accttgtatc tggatacctc cggagagccg
    37561 ctttacaaac gcgggttcaa gccggccgca gtcgaggcac cgctgaagga gaacctggcg
    37621 gcgggcatcc tgcgcctgac cggttggcaa ccgggatccg aggcgctggt ggatcccatg
    37681 tgcggcagcg gcaccttcct catcgaggcg gcccagatgg ctctggatgt ggcgccgggc
    37741 ttggggcggc gctttggctt cgagaagttc aagaacttcg agcgcgacca gtggttgccg
    37801 atccgccagg ccgccgaggc ccggcgccag gcgccgcgcc ccttgcccat ccatggctcc
    37861 gacatcctcg aggcccagga acgcaagagc atcgtcaatc tgcgcagcgc gggcctggac
    37921 ggctgcgtga cggtctcgcg caccgacatg ctggaactgg cggcgcccgc ggcgagcggc
    37981 gtgctggtcg ccaatccgcc ctacggcgtg cgcctgtctg acatgtcgga actgcgcgac
    38041 ttctatcccc gcctgggcag cgccctcaag gcccactggg cgggctggcg ctgctacttc
    38101 ttcagcggcg acccggagct gcccaagggc gtccgtctca aagccagcaa gcgcacgccg
    38161 ctcttcaacg gcgccatcga atgccggttg tacgaatttg cgatggtggc cggttcggcg
    38221 cgcaagccga agggggaagg cggcgccgcg gacgcggatt gaccggcggc cgaagcgtgg
    38281 cagaaagcgt cgcgagcgcg tcggggtcgt cgtgagaaac gcggccgtaa atggggtacg
    38341 gcgagtatca ccgcggcgag atcgaggcgc ttagccgctt tgttcggcgc ttcagacgat
    38401 gtcgagattc tggatgccgg acaacatgtc gttgtctccc cgccggctct tgagcttgat
    38461 ctgcagacgc aggtcgttca tggagtcggc gttgcgcagg gcgtcctcgt aggtgatgag
    38521 gccgcgttcg tagaggtcga acaggctctg gtcgaaggtc tgcatgccca gttcggccga
    38581 gcgcttcatc acgtccttga tgccgggaat ctcgcccttg aagatcagat ccgagatcag
    38641 cggcgagttg agcatgacct ccactgccgg tacccgaccc ttgcggtcgc acatgggcag
    38701 caggcgctgg gagatgaggg cgcgcaggtt gagggagaga tccatcagca actgggcgcg
    38761 acgctcttcc gggaagaagt tgatgatccg gtcgatggcc tggttggcgc tgttggcgtg
    38821 cagggtggcc aggcacaggt gaccggtttc cgcgaaggcg atggcgtagt ccatggtctc
    38881 ccggtcgcgg atctcgccca tcaggatgac gtcgggggcc tgccgcaagg tgttcttgag
    38941 ggctgcttcc caggagtcgg tatcgatgcc cacttcccgc tgggtgacga tgcagttctt
    39001 gtggggatgg acgaactcga ccggatcctc gatggtgatg atatggccgt aactcttctc
    39061 gttgcggtaa tccaccattg cggcgagtga ggtggtcttg ccggtaccgg taccgcccac
    39121 aaagaccacc agcccgcgct tggacatggc aatgtccttg agcacgggcg gcaggccgag
    39181 ttcggtgaag ttgggaatgc gctggggaat cgttcgcagc accatgccga cctggccttg
    39241 ctggatgaac gcgttagccc ggaagcggcc gatgccggcc ggcgagatgg cgaagttgca
    39301 ctctttggtg gcttcgaatt ccgcggcctg gcggtcgttc ataatgaccc gtgccagttc
    39361 ggccgtgtgg gctgcgctga gcacctggtt ggactggggc gtgatcttgc cgtccacctt
    39421 gatggcgggc gggaagccgg tggtgatgaa gaggtcggac ccgttcttct gcatcatcag
    39481 cttgagcaga tcgtgcatga acttgagggc ctggtcgcgt tccatggcgc attcctgtcg
    39541 ggtgaaggcc gatccgggcg tcggctgtta agaattgtaa atcactccac cattttgatg
    39601 agtcacccgg tgaaggaatc cttgttggcg gcatagacgc gggcttccac cgccgagatc
    39661 tggttgcgcc ggatcaggtc cgtgaggctc tggtcaaggg tctgcatgcc caggttctg
//
LOCUS       DAOQPA010000017        39462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_409_length_39462_cov_18.579237, whole genome shotgun sequence.
ACCESSION   DAOQPA010000017 DAOQPA010000000
VERSION     DAOQPA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 39462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39462
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_409_length_39462_cov_18.579237"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKX01_04100"
     CDS             complement(<1..274)
                     /locus_tag="PKX01_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75337.1"
                     /translation="MNVRLAVLPLLLLAGCATLPDGPRVAVMPAPGKPFDLFVQEERA
                     CRAFASQSLGTTPNDLAARNVAGTAIVGTAVGAAAGALAGGHNGAGA"
     gene            complement(271..741)
                     /locus_tag="PKX01_04105"
     CDS             complement(271..741)
                     /locus_tag="PKX01_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75338.1"
                     /translation="MNPRTIRRALLAAAAGIALMATGAAFADRGHGGGWHGAPGGWAA
                     RPAPGGYWHYGEHRGAWGWWWVLPGAWWLYDRPYYPYPAAPPVVVQPVPQEPVIVQPA
                     PQPAAQTWYFCESARAYYPYVQTCAEGWRPVPAAPPPASGAQQSAPAAGSGGVQ"
     gene            966..1382
                     /locus_tag="PKX01_04110"
     CDS             966..1382
                     /locus_tag="PKX01_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75339.1"
                     /translation="MDVRRLVMGLGVLIVVGVGAVAVLGPELSGARAANGGGALAQAV
                     ESEYGDALRNAGVQSVGVDPRTQDVVVVFADVRPGGRPVGRLAGLAGPEGCGETDLGI
                     EVAGQITAWGQAGVRRERMRCVLDAVVTRMAVLRGS"
     gene            1429..1816
                     /gene="rnpB"
                     /locus_tag="PKX01_04115"
     ncRNA           1429..1816
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKX01_04115"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2198..2644
                     /gene="mraZ"
                     /locus_tag="PKX01_04120"
     CDS             2198..2644
                     /gene="mraZ"
                     /locus_tag="PKX01_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMZ75340.1"
                     /translation="MFQGAAALSLDAKGRLAIPARHRDALAAQGDGQLVLTAHPHRCL
                     LIYPAPAWEPIRDKVLAGSSLEQHSALLKRLLVGFARDEELDSAGRVLIAPELRAFAQ
                     LDKQVHLVGQGNHFELWSDAGWQKQQEQIFALGDQLMPANWDALPL"
     gene            2641..3573
                     /gene="rsmH"
                     /locus_tag="PKX01_04125"
     CDS             2641..3573
                     /gene="rsmH"
                     /locus_tag="PKX01_04125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMZ75341.1"
                     /translation="MTTSAPHVTVLLEEAVEALAVKPDGLYVDGTFGRGGHSRAVLAK
                     LGHQGRLIAFDRDPVAIAAGRGLDDPRFELVHAPFSDFAEALDARRVAQVDGVLLDLG
                     VSSPQLDEAERGMSFRFDAPLDMRMDTSRGETAAEWLAQAPVAEITRVLREYGEERFA
                     YEIAKALAAARAGGHVATTGQLAAIVEKAVRTREPGQHPATRTFQALRICVNRELEEL
                     SLILPQAVTRLVPGGRLVVISFHSLEDRIVKRFMRDESRPPQLPARLPLRAAELPRPR
                     LTLVGRQVRPSDAEVAANPRARSAVMRVAARTEA"
     gene            3575..3841
                     /gene="ftsL"
                     /locus_tag="PKX01_04130"
     CDS             3575..3841
                     /gene="ftsL"
                     /locus_tag="PKX01_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HMZ75342.1"
                     /translation="MARLTGFLIMLIVASSLGVVASQHRARKLYAAVEREQQRTRQLE
                     VEWDQLQLEQSTWAAHARVEKLAREKLSMRAPQPGQIVAIEERP"
     gene            3838..5592
                     /locus_tag="PKX01_04135"
     CDS             3838..5592
                     /locus_tag="PKX01_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ75343.1"
                     /translation="MNKTQKGVTFNHNPLLRSELPAWRPRFVLLCLLGGSLALVGRAA
                     YLQGVNDRFLQAKGESRYARVLDVPATRGKITDRQGEPLAVSSPVKSIWAIPEDAKLT
                     PAQSRQLAGLLEMPVQDLNQKLGTDKDFVYLKRQVSPELADRIAALKLPGVHDQREYR
                     RFYPGGEVMAHVIGFTGVDDKGQEGIELAFEPQLAGKPGSRRVVKDRRGNVVEDVQSI
                     REPQDGREVALAVDNKIQYLAHAALKQAMTDHKAKAGGIIVIDSRSGEILALANAPTY
                     NPNNRTKLTGAQLRNRALTDTFEPGSTMKPFSAAMSLEAGKVTANTIINCAPGRMTIG
                     TATISDAHPHGALTVAQVIQKSSNIGAAKLALAFPPETMWKMFDDLGFGAPLHLGFPG
                     EVGGRLRPAKSWRPVEQATMSYGHGISVSLMQIANAYQVFARDGDMVPLSLARVDAPP
                     VHGKQVFSPHTAREVRQMLELVVQQGGTAPQAAIPGYRAGGKTGTAMKLEGGHYTTKY
                     VASFVGIAPISDPRLIVAVMIDEPTNGKHYGGDVAAPVFAQVMGGALRTLGVAPDAPL
                     KPLQVARQSVVPAVKESM"
     gene            5589..7100
                     /locus_tag="PKX01_04140"
     CDS             5589..7100
                     /locus_tag="PKX01_04140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMZ75344.1"
                     /translation="MSPEHQASDILARLAADGVEATRLCADSRQVLAGDIFVAYPGHR
                     ADGRRYIVDAVHAGASAVLWEREGYQWNEAVDVPNVPVFGLAHLSGYIADEFYGRPSQ
                     ALWTVGVTGTNGKTSVTQWLARAYAQLGRRCGLIGTLGIGFPGQLSATLNTTPDALTV
                     HRALADIRDQGGQAVAMEVSSIGLDQARLNGVRIDVAVFTNLTRDHLDYHGTMEAYGE
                     AKARLFAMPGLRSAVLNLDDRFGMEQARSLAGSGVEVIGYTVIPSNRDAAPAQRVLVA
                     ENLTITPVGLRFTARCGNVSADLFANVVGRFNASNLLAVIATLMASGIPFEDACKVAE
                     GLTPPQGRMQTLGGVGDPLVVVDYAHSPDALEQVLRAVRPTAEARGGSLTCVFGCGGD
                     RDPGKRPLMGEVARRLADRLILTSDNPRTEDPQRILQAIVAGVQGDVDIIPDRAEAIR
                     HAVMRAAADDVVVVAGKGHEPYQEINGVRLPFSDAEQTRAALEAWNDAQGGLS"
     gene            7100..8473
                     /gene="murF"
                     /locus_tag="PKX01_04145"
     CDS             7100..8473
                     /gene="murF"
                     /locus_tag="PKX01_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMZ75345.1"
                     /translation="MDLQGAAVLTRGTAVGGNVVFESVSTDSRALQSGQLFVALRGER
                     FDGHGFVAQAASQGAAAAMVDRAWAAANPVPLPLLVVDDTRIGLGQLAAGWRARFELP
                     LIGVTGSNGKTTVKEMCAAILRARARREGLDPDLAVLATRGNLNNDIGLPLMLLRLSG
                     NHRAAVIEMGMNHPGEIGYLTRLAAPTVALVNNAQRAHLEGMGSVNEVAREKGAIYEG
                     LSADGVAVVNADDAFAGYWKALNAGRRVLTFGLRPGADVTAQVTSHGFASELTLASPK
                     GAASFRLQVPGEHNVRNALAAAAACLAAGARPEDVAAGLGAFSGTPGRLHVRAGVGGA
                     TVIDDSYNANPDSMRAGIAVLAATPGRKILVVGDMGEIGDQSFQYHDEIGGYAKSEGV
                     DFLFALGEMSEVAARNFGDGGRHFQRVDELVAAVKAEMNSNTVVLVKGSRFMKMERVV
                     DAIAEKA"
     gene            8534..9622
                     /gene="mraY"
                     /locus_tag="PKX01_04150"
     CDS             8534..9622
                     /gene="mraY"
                     /locus_tag="PKX01_04150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ75346.1"
                     /translation="MLLELAQWLSKDARFFNVFGYITLRTVLAAGTALLLSLVFGPPV
                     IRWLAAKKIGQAVRDDGPKSHLTKAGTPTMGGALILISIAVTTLLWGDLRNKYVWVVL
                     LVTLGFGAVGWVDDWRKVVYRDPKGLASRWKYFWTSMVALGAATFLGLTATLPAQIEL
                     IVPFFKAVTYPLGVVGFIVLSYFVINGTSHSVNLTDGLDGLAIMPTVMVSAALAIFAY
                     VAGHMGFSKYLGMPYIPGAGELAVICGAIAGAGLGFLWFNAYPAEVFMGDVGALALGA
                     AMGTIAVIVRQEIVLFIMGGLFVAEALSVMVQVMYFKYTGGKRIFRMAPLHHHYELGG
                     WKETQVVVRFWIITIMLVLFGLSTLKLR"
     gene            9629..11017
                     /gene="murD"
                     /locus_tag="PKX01_04155"
     CDS             9629..11017
                     /gene="murD"
                     /locus_tag="PKX01_04155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMZ75347.1"
                     /translation="MAADLTLILGLGESGLAMARWLARQGVALRVADSRERPPGVERL
                     RAEAPAAEVVAGPFSDALLEGVTRIAISPGLDPRQPLVATARARGLTVVGEMDLFVQA
                     LRDLGVRDATRLIAITGTNGKTTTTTLAGEMVRACGVDGVVAGNISPAALDVLMARLD
                     AGEALPAVWVLELSSFQLETHPALEAISATVLNVTDDHLDRYAGLDDYAATKAAVFQG
                     AGVQVLNREDARVAAMAVPGRRVYSFGVDAPAGPDDFGLRTDAGGEWLVRGDERLLAR
                     ADLPLPGNHNIANALAALALCEAAGLPRAPLLAALKAFRGLPHRVEKVARRADGVVYY
                     DDSKGTNVGATVAALEGLRARVVLIVGGDGKGQDFSPLKDAFARYARAVVLIGRDAPA
                     IEAAVAGCGVPLIPAADMDAAVLAADARAQAGDVVLLSPACASLDMFRNYAHRAQVFI
                     DAVRRLPGVRPA"
     gene            11014..12249
                     /gene="ftsW"
                     /locus_tag="PKX01_04160"
     CDS             11014..12249
                     /gene="ftsW"
                     /locus_tag="PKX01_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HMZ75348.1"
                     /translation="MSLTASLSGFFSRHAGAASQSEASRAVRRLMRPLGQPRELDPLL
                     IWSAAALLLLGLIMVFSASIATAEGSRSFSYQPAYFLIRHTVFLAIGVIAGVVVFQVP
                     MRTWQEIAPWLFIAGVVLLLVVLIPHVGRDVNGARRWLPLGVVNLQPSELMKLFAALY
                     AADYTVRKLPYMGSFKKGFAPMAAVILFVGFLLLKEPDFGAFVVITAIAFGILFLGGI
                     NVRLFVLLAVVAAVGFVLLITFSEYRRQRILGFMDPWQDAYGKGYQLSHALIAFGRGE
                     WFGVGLGGSIEKLFYLPEAHTDFLLAVIAEELGFAGVFTVVVLFGILIHRAFAIGKQA
                     LVLERTYAALVAQGIGIWIGVQSFINMGVNMGLLPTKGLTLPLMSFGGSGILANCVAL
                     AILLRIDWENRQITRGGNA"
     gene            12249..13307
                     /gene="murG"
                     /locus_tag="PKX01_04165"
     CDS             12249..13307
                     /gene="murG"
                     /locus_tag="PKX01_04165"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HMZ75349.1"
                     /translation="MPTLMVMAGGTGGHIYPGLAVADALRARGWKVVWMGNPDGMEAR
                     IVPASGYDMAWVRFTALRGKGLLRKLLLPFNLLRGFAQALAQLRRVRPDVVLGMGGYV
                     TFPGGMMAALIGRPVVVHEQNSVAGLANRVLAGVADKVMTGFPDVLRKGEWAGNPVRA
                     DIAAVAPPAQRFAGRSGPLRVLVVGGSLGAAALNDAVPKALALIPPAERPQVTHQAGT
                     RQIDALRAAYRAAGVEGELLPFIDDMAERYAAADLVICRAGALTVAELAAAGVASVLV
                     PFPHAVDDHQTGNARFLADAGAAVLLPQTELDPARLAALLGSLNRERLLEMAEKARAR
                     ARPDATSRVADVCAGLAA"
     gene            13318..14712
                     /gene="murC"
                     /locus_tag="PKX01_04170"
     CDS             13318..14712
                     /gene="murC"
                     /locus_tag="PKX01_04170"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMZ75350.1"
                     /translation="MKHKVKSIHFVGIGGAGMSGIAEVLVNLGYQVSGSDLGDNAATR
                     RLKELGVRVMQGHAAENVQDADAVVTSTAVKGDNPEVIAARARQIPVVPRAQMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASILAEGGLDPTFVIGGKLNAAGANARLGKGDFLVAE
                     ADESDASFLLLTPVISVVTNIDADHMETYGHDFARLKQSFVDFLQHLPFYGVAVLCDD
                     DKHVRSIMPLVSKQIVRYGFSESANVRAENIHAEDGRMRFDVVRVNGTTSRISVVLNL
                     PGRHNVLNALAAIAVATEVGVSDEAIVKALADFKGVGRRFQRYGEVALPAGGAATVID
                     DYGHHPVEMAATLAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLSTADALLLGE
                     VYAAGEAPIVAADGRSLARALRVAGRIEPVFVEHIEDMPQAILAAAQDGDVVITMGAG
                     SIGAVPGKLVNGEV"
     gene            14724..15638
                     /locus_tag="PKX01_04175"
     CDS             14724..15638
                     /locus_tag="PKX01_04175"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ75351.1"
                     /translation="MRSQFGKVAVLLGGSSAEREISLMSGKAVLAALQGVGVDAHAFD
                     PAETDLHILKEEGYERAFIALHGRGGEDGTVQGALELMGIPYTGSGVMASAIAMDKWR
                     TKLVWLASGLPTPRFAILTADSDWDAVVAQLGLPIFVKPVHEGSSMGATKVTEAAQLK
                     GAWELAAQYDSLVLAEEFVSGQELTAPFLGDRALPLVRIVAPDGNYDYQHKYFTDDTQ
                     YFCPSGLPEALEKDIQATIVRCAKVLGCRGWGRADLILQDDGRYTLLEINTAPGMTNH
                     SLVPMSARVSGLSFEALCLEILAGARLG"
     gene            15631..16509
                     /locus_tag="PKX01_04180"
     CDS             15631..16509
                     /locus_tag="PKX01_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HMZ75352.1"
                     /translation="MVDRRATSNVPPEAGAKPSLPRRPRPAARAQKRDGGGNGLWDRP
                     QLLNLIADLLLVAGAAGLGYAAVAGLSRLPAFPLKEVVVTTPLAQVTTEQLEYAARSS
                     LKGNFFTVNLEEVRNAFEKLPWVRRADVRRRWPGGVEVRLEEHVASAYWRVGDTGDMR
                     LVNRYGEIFSAASNAPMPIFSGPEGSSGVLLAKFDEFSHKLGPLGRGLVGVSLSAREA
                     WQLKMADGLTIELGHDQAKAPLDERIDRFVRYYPRAKAQLNMNVAVVDLRYPNGFALR
                     AVPVAAPAGKQDSKGK"
     gene            16509..17738
                     /gene="ftsA"
                     /locus_tag="PKX01_04185"
     CDS             16509..17738
                     /gene="ftsA"
                     /locus_tag="PKX01_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMZ75353.1"
                     /translation="MSKEYKELIVGLDIGTSKITCVVAELRPDGRLDVIGLGTQPSSG
                     LKKGVVVNIESTVDAISRVIQEVELMADCKIRDVYTGIAGSHIRSFNSNGMVAIKDKE
                     VSPMDVERVIETARAMPLPADQQILHILTQEFIIDGQDGVREPLGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVLDLVLQPLASSYATLSEDEKELGVCLVDIGGGTTDLA
                     VFTQGAIRHTAVIPYAGDQITNDIAMALRTPTAEAEEIKIRHGVAMHTLTDPAEMIEV
                     PGVGDRPPRKLSRQALADVIEPRVSEIYELVQAELRRSGYEELLSSGIVLTGGSAVMQ
                     GMVELGEEVFHMPVRIGYPNYSGALAEVVCEPRYANAMGLVLEGAAQKRRGIQARQTR
                     NVGQVFSRMRSWFERNF"
     gene            17879..19018
                     /gene="ftsZ"
                     /locus_tag="PKX01_04190"
     CDS             17879..19018
                     /gene="ftsZ"
                     /locus_tag="PKX01_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMZ75354.1"
                     /translation="MFEIVEKASGGTSIKVIGIGGAGGNAVDHMIRQGVQGVDFICAN
                     TDAQALTRCLAPVKVQLGKTGLGAGSKPEAGRAAAQESREQIAAALEGAHMCFITAGM
                     GGGTGTGAAPVVAEVAKEMGVLTVAVVTKPFDFENRLRVAESGVEELSRQVDSLIVVL
                     NDKLLEVYGDDAGFEDCFRSADNVLTSAVGGIAEIINVPGLVNVDFQDVRTAMAEMGR
                     AMMGSAEAAGLDRARIAAEQAAVSPLLEGTELSGARCVLVNITASRSLKMSEVRDAVK
                     TVQAFAAPEAFVKYGTVFEENMEDRIRVTVVATGLGAVRAAVARKPEMQVLRTGTDNI
                     GVEVDYGNLELPAVIRRTSRTTVEAMSASGVSTLDIPAFLRKQAD"
     gene            19177..20094
                     /gene="lpxC"
                     /locus_tag="PKX01_04195"
     CDS             19177..20094
                     /gene="lpxC"
                     /locus_tag="PKX01_04195"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HMZ75355.1"
                     /translation="MLKQRTLKSAISTTGVGLHSGNKVTLSLRPAPPDTGVVFHRVDL
                     DPPVDLPADPYAVCDTRMCSGLQKGTAKVNTVEHLMSALAGLGVDNVHIDVDAAEIPI
                     LDGSAGPFVFLIQSAGIEEQNTPKRFMRVIKPVEYREGDKWVRLEPYDGYRLSFSILF
                     NHPAIDRTGTSVTVDFAEHAYTREVARARTFGFMQDVEFMRANGLGLGGSLDNAIVMD
                     EYRVLNADGLRYADEFVKHKVLDAIGDLYLVGHPLLAAYSAYKSGHALNNQILRVLLE
                     DRSAWEIVTFEEDARTPPAVAHLFDLALA"
     gene            20109..20300
                     /locus_tag="PKX01_04200"
     CDS             20109..20300
                     /locus_tag="PKX01_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75356.1"
                     /translation="MLLLRLIAVLVAIGIGASAFAYLFTRDRRYLAFALRLARYALIV
                     ALVFLALLALERVLAPAFG"
     gene            complement(20304..20732)
                     /locus_tag="PKX01_04205"
     CDS             complement(20304..20732)
                     /locus_tag="PKX01_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMZ75357.1"
                     /translation="MARALDAYLDRGDTLARLHAHAARLLRLQRTLSDVLPDYLAASC
                     QVANLKDEVLVIHTRGAAVAVKLRQAIPGLLADFARAGVALRDIKVKVAAPESPRPAA
                     APVHRSVSDGTRAGLDELAGRLPADSPLAQALRRFVRSAG"
     gene            20882..23608
                     /gene="secA"
                     /locus_tag="PKX01_04210"
     CDS             20882..23608
                     /gene="secA"
                     /locus_tag="PKX01_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ75358.1"
                     /translation="MIAGLLKKIFGSRNDRLIRQYAQKVRVINALEPTVQALSDEALR
                     GKTAEFRQRLANGEALDALLPEAFAVVREAGKRVHGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLTATLPVYLNALAGKGVHVITVNDYLASRDSDWMGRIYRFLGMSVGCNLSQ
                     MPHELKKAAYEADITYGTNNEFGFDYLRDNMVYSLEERVQRGLHYAIVDEVDSILIDE
                     ARTPLIISGQAEDHTELYIRMNAVPPLLTRQEGEGEDITQPGDYTVDLKAHSVLLTEQ
                     GHEHAEEILGRLGLLPEGASLYDPAHILLIHHLYAALRAHALYHKDQHYVVQNGEVVI
                     VDEFTGRLMPGRRWSEGLHQAVEAKEGARIQAENQTLASITFQNYFRMYGKLSGMTGT
                     ADTEAYEFHQIYGLETVVVPTARPMVRKDQNDKVYRTMKEKHDAILADIQDCVQRGQP
                     VLVGTTSIENSEFLSDLLTRAKLPHNVLNAKQHAREAEIVAEAGRPGVITIATNMAGR
                     GTDIVLGGNVDKPIALLREDENIPDADKEAQISRLREEWKVRHEQVLQAGGLKIIGTE
                     RHESRRIDNQLRGRSGRQGDPGESRFYLSLEDPLMKIFAGERLNAIMVRLKMPEGEAI
                     EAGMVTRSLETAQRKVEQRNFDIRKQLLEYDDVANDQRKVIYQQRNELLESTDITETI
                     TAMREGVLHDLLRTYVPAESVEEQWDIPALELALQSENQLKLPVGDWLKAEPDLTDDD
                     LLQRILEAGNAAYADKISLVDAAAWHGFERNVMLQSLDTHWREHLSALDHLRQGIHLR
                     GYAQKNPKQEYKREAFELFENLLSVVRVEVTKLLMTVQIRSEAQLDEAEAPPELENVQ
                     YHHADYDEALATANPDAGVAQPAVNLTPKVGRNDPCPCGSGKKYKHCHGKLN"
     gene            23720..25150
                     /locus_tag="PKX01_04215"
     CDS             23720..25150
                     /locus_tag="PKX01_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ75359.1"
                     /translation="MTLPAHPLDLTGWIERIRGRDMPVFGRTVESLRTLLSDDRVSAS
                     TLAQVILKDAPMTAKVLRLANSAFFNTSRQTVSTVSRAIVVLGFNPVAQIALSVSLID
                     ALLSGGVRGRAEAEMARSFHAAVQARWAAAKRGEGHGEEVFIAALLTRVGEMAFWCFG
                     GDQARSLDGLLAQGRMREEDAQQVVLGFPLRHLSAGLVREWHLGALAQAVLEGQSRGR
                     NGEQAVLLGHRLARASEQGWDAPPARQAVQALADYLELPFAAVLDAARANAAEAARIA
                     SSFGAPAPPAGAAASAGSGPAQADPAAPPVGVADPHLQLHILHDVAAMTLAQAPLDDI
                     LQMVLEGLYRGVGCERVLVARLSADRSELQGRVAIGSGAEALAASFVFSMDGEPDDLL
                     DAAIDSAQGCWLTPSRAAQPGAERLLKVAGSRHAYLAPFGTGSRQVGVFYADGHGRAL
                     DEAGWHAFQHFVLQASLAVGLSLQGQ"
     gene            25294..26532
                     /gene="argJ"
                     /locus_tag="PKX01_04220"
     CDS             25294..26532
                     /gene="argJ"
                     /locus_tag="PKX01_04220"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HMZ75360.1"
                     /translation="MPVLLSTPDPVCLLPVPGVRLGVAEAGIRKANRRDLTLIELAPG
                     SRVAGVFTTNRFCAAPVQVCKAHLPAGTIRALVINTGIANAGTGEPGLAAARATCEAV
                     AAQLGLAADQVLPFSTGVILEPLPVERLVAGLPAARAALRADGWFDAAHAIMTTDTLA
                     KAVSRQVQVGGKTITLTGMSKGAGMIKPNMATMLGFLATDADVAQPVLDALLKEVTDL
                     SFNSITVDGDTSTNDSYILIATGQAGNGTLTDTASADYAAFREAVLDVSIRLAQAIVR
                     DGEGATKFMTLQIGGGRDREECRKVGYAIGHSPLVKTAFFASDPNLGRILAAIGYAGI
                     ADLDVAALRVWLGSQGEEVLVAEQGGRAASYREEDGARIMQAAEITVRVDLARGDAAA
                     TLWTCDFSYDYVKINADYRS"
     gene            26549..27202
                     /locus_tag="PKX01_04225"
     CDS             26549..27202
                     /locus_tag="PKX01_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75361.1"
                     /translation="MKTSPLDYVLSTIAITLILLLAVAGVWALRPLTTAALADYHVVA
                     DFLLLLLFYGLLSAVAVRVLLRVQPIAPGEYDGDSPVFTFWKLITIVYRLGQGALLPF
                     TTVFTRPVVEALYGARVGADVAIGGTIDDPYQVEIGPRAVIGHNALVSGNVLYGGKLT
                     CGKVIIGAGATIGVNSVVLPGSEIGENAVLMGGSYLMPGTKVPAGETWRGNPARKWM"
     gene            complement(27270..28091)
                     /locus_tag="PKX01_04230"
     CDS             complement(27270..28091)
                     /locus_tag="PKX01_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ75362.1"
                     /translation="MNARSALYQARYGNPDVAVPAAASPVIDHLLAHASVRAYLPDPV
                     SDDTLAAIVAAAQSAASSSNLHAWSVVAVRDAARKVRLADYAGDQAHIRQAPLLLVWL
                     ADLARLEQVARRVGETSAALDYLEMFEVAVIDAALAAQNAVAAAESLGLGTVYIGAMR
                     NRPEDVAAELQLPPRVVAVFGLCVGMPDPAAPAAVKPRPPQSVVLHQETYSLPAQEAG
                     LETYDQAMAAFYAAQGMKVRGTWSNHSAKRIRDAASLTGRDRLAEALRNLGFPLR"
     gene            complement(28201..28773)
                     /locus_tag="PKX01_04235"
     CDS             complement(28201..28773)
                     /locus_tag="PKX01_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75363.1"
                     /translation="MYLSPDEIHQNRLDTLHAIEAASQAWIDATGKLSELILRAGRQM
                     LDESERHLSQLAQPGASLPLSIGSLDAWRSDSAELVRDSFAIIAQAHQTTLQIARKQV
                     GIFDLALKRGMAHAIQHADAETGSAISHVRTALEQAETCLNELTDSATRGVGAMEEQV
                     RQIAEALASTADEAPPAAPAPRRTRKPRAQ"
     gene            complement(28877..29659)
                     /locus_tag="PKX01_04240"
     CDS             complement(28877..29659)
                     /locus_tag="PKX01_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HMZ75364.1"
                     /translation="MAFFDNLTLNLGDGFRKYFQISAAFDNAGRDDVFAIRHEVYCEE
                     LGFEPVRANRRETDEYDRHSRHCLLRTSTEPHTLVGCNRLVLARPEDPAYPLPFERTC
                     AATLDRRIIDPAKEPRGAIAEVSRLAVRGLYRRRKGEARSEVALSDSDFGTAAQPRFP
                     YIPISLYLAVVAIAQREGVETLFVLTEPRLADHFSKLGVKIRQIGGPVEHRGTRIPSM
                     MDVKGIIGGLRFFMKPMWRVVNEQIDAGYTSGSPDLPAGSAC"
     gene            complement(29685..30899)
                     /locus_tag="PKX01_04245"
     CDS             complement(29685..30899)
                     /locus_tag="PKX01_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75365.1"
                     /translation="MPFTLAIRNILRQKFRTGMTLAAIIFGVVGLILAGGFVEDIFIQ
                     LGETIIHSQTGHIQVFKKEFLEKGIRYPERYLIDHPEDIARHFDGHPEVEAVMSRVYF
                     SGLLNNGRRDLPIIGEGVEPDKETKLGSLLKITEGQPLTDKDPDGILIGQGVAQSLAV
                     KPGDALTLVTSSAGGAMNTHDFRVVGIFQSFSKEFDARAVRIPLNAGQDLLQTRGVNQ
                     VVVALHRTDDTDAVLKRASVELADSPYDVRSWRTLSDFYDKSVQLYDTQFGVLRLIIL
                     LMVLLSVTNSVNMTVYERQGEFGTMQALGNRARDVVRLVVLESALVGLGGALAGVVLG
                     VIAALIISAIGIPMPPPPNANVGYTAFIRVVPGTLAGAFLVGFVATVLASLFPAYKVS
                     RLPVVDALRQNV"
     gene            31086..31952
                     /locus_tag="PKX01_04250"
     CDS             31086..31952
                     /locus_tag="PKX01_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HMZ75366.1"
                     /translation="MVQFVYDEAFSRNIGWVTVAEQAALRGKRVAIAGMGGVGGVHLL
                     TLARLGIGAFHIADMDVFELVNFNRQVGATVSNLQRPKAAALAEMARDINPELDIRLF
                     ERGVDDANLGDFLDGVDLYVDGLDFFAFDARRATFRACAARGIPAVTAAPLGMGTAVL
                     SFLPGQMSFEDYFCLDGCDEEEMAIRFLLGLSPAMLQRGYLADPSRVNLAEQRGPSTI
                     AACQLCAGVTAVESLKILLGRGDVLCAPRGFQFDAYRNRYVKTWRPGGNRNPLQRLAL
                     FIARRQLRAMRR"
     gene            32057..33133
                     /locus_tag="PKX01_04255"
     CDS             32057..33133
                     /locus_tag="PKX01_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ75367.1"
                     /translation="MIPRDTRLQILDLARWAPSGDNTQPWRFEIAGDDRLVVHGHDTR
                     TWCLYDYNGHASHMAHGALLETLRIAATAHGLEAVWRLRADSTDTAPVYDVTLSPVSG
                     LAADPLLSCIERRTVQRRAMRATPLDSGQRTALAAAPGAGYTVQCFETGAERRAVAGL
                     LWRNAYLRLTCPEAYPVHKDVIEWRARFSKDRIPEQAVGVDAVTARLMEWVMASWGRV
                     QFFNRFLGGTIAPRVQLDLIPALACSAHLLLKADVPPRDLADHVRAGAAMQRLWLTAT
                     AVGLHLQPEMTPVIFRWYARSGQRISHDPAIDRGAAALAERFEQIAAIGPDQALSFFC
                     RVGVSDPPRSRSLRKGLDELLLKP"
     gene            33130..34080
                     /locus_tag="PKX01_04260"
     CDS             33130..34080
                     /locus_tag="PKX01_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ75368.1"
                     /translation="MSHVHGPGCRHGHHHGHAHGAHGQGPAHHDRAFAVGIVLNVLFV
                     AVEAWFGWRADSLALLADAGHNLSDVAGLALAWGAAFAARLRPTHVRTYGWQRASILA
                     ALANAILLLVAMGALALEAVQRLGAPHPGDAVTVMAVAALGVLINGLSAALLMKGSQD
                     DLNIRGAFLHMAADALVSLGVVGAGALYLAFGWAWLDPLVSLAIAALIVAGTWGLLRQ
                     SLHLAFDGVPEGVDLVEVHAWLRSQPGVRSVHDLHVWALGTSDVALTAHLVMPAGHPG
                     DAFLIALADGLVHRFRIGHPTIQIEIDGLPDGCVRPARLE"
     gene            34389..35246
                     /locus_tag="PKX01_04265"
     CDS             34389..35246
                     /locus_tag="PKX01_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HMZ75369.1"
                     /translation="MAPTAVRTADAESLSAAPRVVAPTDRGSVRSRASRWPARLDVLQ
                     SVSGLFLAVFLIAHMFFVSSILISKDAFYTVARFFEGAWLLGRPQPWLVSCVVGFVLL
                     VFVGHAWLALRKFPANFRQYRAFAAHRAGLRHDDTGLWWIQLWTGFALFFLGTVHLYG
                     MLTQPELIGPYESADRIWTGTLWPLYLLLLFAVEIHGSIGLYRLAVKWGWLEGRDAAV
                     SRRRLRVAKTVFSAFFLILGLITLRAYVQIGVTHADRAGERYVPGAHATLVPNPSALA
                     AAPNPPSME"
     gene            35249..37228
                     /locus_tag="PKX01_04270"
     CDS             35249..37228
                     /locus_tag="PKX01_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HMZ75370.1"
                     /translation="MRIVYTDVLVIGGGLAGLRAAVGARRRGHEVILLSLVPPKRSHS
                     AAAQGGMQASLGNTAMGDGDNEDVHFEDTVRGSDWGCDQEVARLFVHTAPQAVRELAA
                     WGVPWNRVQAGPHEVVMDGRRVTLTERDSAHGLIAARDFGGTRKWRTCYVSDGTGHAM
                     LYAVGDQAVAAGIPVHERTEALALIHEGGRCHGAIVRDLVTGALSAYVAKATCIASGG
                     FGRIYQVSSNAIINEGIGAAIALETGVATLANMEAVQFHPTTIFPAGILVTEGCRGDG
                     GLLRDAAGHRFMPDYEPEKKELASRDVVSRRMEEHIRAGHGVKTRYGDHLWLDITLLG
                     AAHIDGRLREVKEICQHFLGIDPSRDWIPVRPAQHYSMGGIRTDASGHVRGLRGLFAC
                     GEAACWDLHGFNRLGGNSVAETVVAGMIVGERMADFVESPMGELQVSTALVRDCLERT
                     QHHLDTLLSGTGTEDAQALLARMQAIMTEQVGIFRRGDLLESAVAELASLLERSRRIG
                     IATRRPGANPELVTAYRVQKMLKLALCVADGALQRTESRGAHFREDFPRRDDALWLKR
                     TLATWPDPQQTRPTLSYEALDVMRMELPPGWRGYGARNAVEHPDTARRADEIATIRSA
                     FATADRYTVQQALMPYEHLVPTRFRGRNHRSGEHT"
     gene            37225..37548
                     /locus_tag="PKX01_04275"
     CDS             37225..37548
                     /locus_tag="PKX01_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ75371.1"
                     /translation="MNAPTPTRIVTLRLADHPDEYALLDTEGLTLLNALNHIRPSLPA
                     PLGDAFVCHAGACATCYVLVNGERCLPCTTFVRDLPDEFTVAPGVHSAGWDEQRHDVR
                     RGHGQ"
     gene            37545..38279
                     /locus_tag="PKX01_04280"
     CDS             37545..38279
                     /locus_tag="PKX01_04280"
                     /inference="COORDINATES: protein motif:HMM:NF010071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HMZ75372.1"
                     /translation="MKPRASAGPRTLTLRILRCGPDIDPHWQDFRIEETEGMTLFIAL
                     TEVRAHQDPSLQFDFVCRAGICGSCAMLINGRPGLACRSLTRDLDPLITLAPLPVFEL
                     IGDLSVNTGKWMRGTAERLETWIHTTAEADLTAVEAPMEPALAEAIYELDRCIECGCC
                     VAACGTLRMREDFVGAVGLNKIARFRLDPRDERSDDDFYELIGDDTGVFGCMSLLGCH
                     DVCPKGLSLQTQIAFLRRKMAVTGLK"
     gene            38414..38551
                     /locus_tag="PKX01_04285"
     CDS             38414..38551
                     /locus_tag="PKX01_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75373.1"
                     /translation="MTTLRIVHRDARANALDHPARFFAGCSLVVLGAGVWCAQAVSHL
                     F"
     gene            complement(38558..>39462)
                     /locus_tag="PKX01_04290"
     CDS             complement(38558..>39462)
                     /locus_tag="PKX01_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75374.1"
                     /translation="EAQRRIAESERAQAEAQRHIAEAERARAEAQRHIAESERARAEA
                     ALAASERELLRAQSAELRALRARLDTLIADAITPGHLTEIAPMQAERALLDQRLADVT
                     RQYLARLAEQIGYRGGFEFLMKWEGNAGRVALIGSSVFIDPLTDLALAKPDDLRRRYE
                     FLLTPAELQALIATAGKRGAEAQAAYAAAPILARIRIRAEDVARLVPDVIEPFWKRLR
                     DKHPILRRPDTPPAVHTALLSVAVNLGARVESLAPFIDAGRWRELADAIDALAARPPV
                     LAGLKRRRAEEAALIRAALAGQPP"
BASE COUNT         6030 a        13267 c        13680 g         6485 t
ORIGIN      
        1 cagcaccggc gccgttgtga ccgccggcga gggcgcccgc cgcggcgccg acggccgtgc
       61 cgacgatggc cgtgccggcc acgttgcggg ccgccagatc gttgggcgta gtgcccaggg
      121 actggctggc gaaggcgcgg caggcccgct cttcctgcac gaacaggtca aagggcttgc
      181 ccggcgcggg catcacggcg acacgagggc cgtcgggcag ggtggcgcag ccggccagga
      241 gcagcagcgg caatacggcg agacggacgt tcattgcaca cctcctgagc cggcggccgg
      301 tgcggattgc tgcgcacctg aagccggcgg cggcgctgcc gggaccgggc gccagccttc
      361 cgcacaggtc tggacgtagg ggtaataggc gcgggcgctt tcgcagaaat accacgtctg
      421 ggcggccggc tgcggggccg gctggacgat gacgggctcc tgcgggaccg gctgaaccac
      481 cacaggcggc gcggccgggt aaggatagta aggccgatca tagagccacc aggcgccggg
      541 cagcacccac caccagcccc acgcgccgcg atgttcgccg tagtgccagt agccgccggg
      601 tgccgggcgc gctgcccagc cacccggtgc gccgtgccag ccgccgccat gtccccggtc
      661 ggcgaaagcg gcgccggtgg ccatcagggc aatgcccgcg gcggcggcga gcagagccct
      721 tcggatcgta cgggggttca tgacctgtac ctcctgttgt cgatactttg attgtgatcc
      781 ccacgaagcc ggcgggcgcc cgccggccgt atcaggatgc aagcgggacg tcgcggcgca
      841 agctttactt acaatttggc ggtggagggc aggtacggcg cccctttgcg gcgcggcgtg
      901 acggatgtcc tcaagcttcc gtcgaggtgt gccgtcaggg cggtcagttt cttcgggagt
      961 gaagcatgga cgtacgcaga ctggtgatgg gactgggggt actcatcgtg gtgggggtcg
     1021 gcgcggtggc cgtgctcgga ccggagctgt cgggagcccg tgccgcaaac ggcggcggcg
     1081 ccctggcgca ggctgtcgag tcggagtatg gcgacgccct gcgcaacgcc ggcgttcaga
     1141 gtgtgggcgt tgatccccgc acgcaggatg tggtggtggt ctttgcggat gtgcgccccg
     1201 gcggccggcc ggtggggcgc ctggccgggc tggcgggacc agagggctgc ggcgagaccg
     1261 acctggggat cgaggtggcc ggccagatca ccgcgtgggg tcaggctggc gtacgtcgcg
     1321 agcggatgcg ctgtgtcctc gatgcggtgg tgacacggat ggcagtgctg cgtggcagtt
     1381 gaggcgggca ccggtgtcgg gcggagcttc gggtatactc cgcgccggga agttcgccag
     1441 gcagtcgcgg tgcgcatgcg catcgaggaa agtccgggcc ccgcagagca ggatgccggc
     1501 taacggccgg gcgccatagc cttcgggtca aagcgacgga aagtgcaaca gagagcagac
     1561 cgccgatggc cccgcaaggg gatcaggcaa gggtgaaacg gtggggtaag agcccaccgc
     1621 ggacgtggtg acacggacgg cacggcaaac cccatccggg gcaaggccaa atagggggac
     1681 gttgacgctg ctcgcggagt ccccgggtag gctgctagag cggcgcggca acgcgtcgac
     1741 cagaggaatg actgcccggt gggtggagtc cgctccaccc gctcgacaga acccggctta
     1801 tcggcgggct tcccaccatt tcccccaatc atcccgcccg ccggggcgcg gccccacttt
     1861 tctccaccgg tcacccggtt cactcctata aatggcgaaa gtccctgtgt cgtcaggggc
     1921 ttccggatac tgccgccata aagccgatga cagcccctgg cgcaggggcg tgtcgccagc
     1981 ttttaaggct ccacttttgc aatcttttct attgccgttg atttataagc ggatttcctg
     2041 ttttgccggt ttttgcacgt aagtgcttgt gtccaaaagg gaaaacggcc cggttcggct
     2101 tgaccgggta ttttttttcc tctaaagtgg ggcaaagtgc accaaagtgg gagaaaaggg
     2161 tttttcccgt ggcggcggtg tgggggagtg ggagaagatg ttccaaggcg cagcagcgct
     2221 cagtctcgat gcgaagggtc gcctggcgat tcctgcgcgg catcgggatg cgctcgccgc
     2281 gcagggcgat gggcagctcg tgctcaccgc ccatcctcac cgctgcctcc tgatctaccc
     2341 ggcgcccgcc tgggagccga tccgcgacaa ggtgctcgcc gggtcgagcc tcgaacagca
     2401 ttccgccctc ctcaagcgtc tcctcgtggg ctttgcccgc gacgaagaac tggattccgc
     2461 cggtcgcgtg ctcatcgcac ccgaactgcg cgccttcgcg cagctggaca agcaggtgca
     2521 tctggtgggg cagggcaatc actttgaact gtggtccgac gcgggctggc agaagcagca
     2581 ggagcagatc tttgccctgg gcgaccagct catgcccgcc aactgggacg cgttgccgct
     2641 gtgaccacgt ccgcgccgca cgtgacggtg ctgctcgagg aagcggtgga ggcgctggcg
     2701 gtgaagccgg acggcctgta cgtggacggc accttcgggc gcgggggcca cagccgggcg
     2761 gtgctggcca agttgggcca ccaggggcgg ctgatcgcct tcgaccggga tccggtggcc
     2821 atcgcggccg gacggggcct cgacgatccg cgcttcgagc tggtgcatgc gccgttttcg
     2881 gatttcgccg aagccctcga tgcacggcgc gtggcgcagg tggatggggt gttgctggat
     2941 ctgggggtgt cgtcgccgca gctggacgag gcggagcgcg gcatgagctt ccgtttcgat
     3001 gcgcccctgg acatgcggat ggatacgagc cgcggcgaga ctgcggcgga gtggctggcg
     3061 caggcgccgg tcgcagagat aacccgagtg ctgagggaat acggtgaaga acggtttgct
     3121 tatgagattg caaaggcgct tgcagctgct cgggcagggg ggcatgttgc aaccactgga
     3181 cagcttgccg cgatcgtgga aaaagcggtc cggacacgcg agccgggtca gcaccctgcg
     3241 acgcgcacct tccaggctct acggatttgc gtcaatcggg agcttgagga actgtcgctg
     3301 atcctgcccc aggcagtgac gcgactggtg cccggaggcc gtctcgtggt gatcagcttc
     3361 cactcgctcg aggaccgcat cgtcaagcgc ttcatgcgcg acgaatcgcg gccgccgcag
     3421 cttcccgcac gcctgcccct gcgggcggcg gagttgccgc gtccgcgcct caccctggtg
     3481 gggcggcagg tgcgcccgtc ggacgccgag gtcgccgcca atccccgtgc ccgcagcgcg
     3541 gtgatgcgcg tggccgcgcg gacggaggcc tgagatggcg cgcctcacgg gtttcctgat
     3601 catgctgatc gtcgccagca gcctgggcgt ggtggcgtcc cagcaccgcg cccgcaagct
     3661 ctacgcggcg gtcgagcggg agcagcagcg cacccgccag ctggaagtgg agtgggatca
     3721 gttgcaactg gaacaaagca cctgggcggc ccatgcccgg gtggaaaagc tcgcgcgcga
     3781 gaagctgtcc atgcgcgcac cgcagccggg gcagatcgtc gccatcgagg agcggccatg
     3841 aacaagaccc agaagggtgt caccttcaac cacaatccgc tgctgcgctc cgaactgccg
     3901 gcgtggcggc cgcgtttcgt gctgctctgc ctgctcggcg gttcgctggc cctggtcggg
     3961 cgcgcggcct atctgcaggg cgtgaacgat cgcttcctgc aggccaaggg cgagtcccgc
     4021 tacgcgcgcg tgctggacgt gccggcgacc cgtggcaaga tcaccgaccg ccagggcgag
     4081 ccgctggcgg tctcgtcgcc ggtcaagtcc atctgggcga ttcccgagga cgccaagctg
     4141 acgcccgccc agagccgcca gctggccggc ctgctggaga tgccggtaca ggatctgaac
     4201 cagaagctcg gcaccgacaa ggatttcgtc tatctgaagc gccaggtctc accggagctg
     4261 gctgaccgca tcgcggccct caagctgccg ggcgtccacg accagcgcga ataccgccgc
     4321 ttctatcccg gcggcgaggt gatggcccac gtgatcggct tcaccggcgt ggacgacaag
     4381 ggtcaggaag gcatcgagct ggccttcgag ccacagcttg ccggcaagcc cggttcccgg
     4441 cgggtggtca aggaccggcg cggcaacgtc gtggaggacg tccagtcgat ccgcgaaccc
     4501 caggacggcc gtgaggtcgc cctggccgtg gacaacaaga tccagtacct ggcccacgcc
     4561 gccctcaaac aggccatgac cgaccacaag gccaaggcgg gcggcatcat cgtcatcgat
     4621 tcccgctccg gcgagatcct cgccctggcc aacgcgccca cctacaaccc gaacaaccgc
     4681 accaagctca ccggcgccca gttgcgcaac cgggccctca ccgacacgtt cgagccgggc
     4741 tccacgatga agcccttctc ggccgccatg tccctggagg ccggcaaggt gacggccaat
     4801 acgatcatca actgcgcgcc ggggcggatg accatcggca ccgccaccat ctctgacgct
     4861 cacccccacg gggcgttgac ggtcgcccag gtgatccaga agtcgtccaa catcggtgct
     4921 gcgaagctgg ccctggcctt cccgccggaa accatgtgga agatgttcga cgacctgggc
     4981 ttcggcgcgc cactgcacct gggtttcccg ggtgaagtgg gcgggcgtct gcgcccggcc
     5041 aagagctggc gtcccgtgga gcaggccacc atgtcctacg gccacggcat ctccgtgagc
     5101 ctgatgcaga tcgccaacgc ctaccaggtg tttgcccgcg acggggacat ggtgcccctg
     5161 tccctcgccc gcgtggatgc gccgccggtg cacggcaagc aggtcttctc gccgcacacc
     5221 gcccgcgagg tgcgccagat gctcgagctg gtggtgcagc agggcggcac cgcgccccag
     5281 gcggcgattc ccggttatcg cgccggcggc aagaccggca ccgccatgaa gctcgaaggc
     5341 gggcattaca ccaccaagta cgtggcctcc ttcgtgggca tcgcgccgat ttccgacccg
     5401 cgcctgatcg tggcggtgat gatcgacgaa ccgaccaacg gcaagcatta cggcggcgac
     5461 gtggccgcgc cggtgttcgc ccaggtgatg ggcggcgccc tgcgcaccct cggggtcgcg
     5521 ccggacgcgc cgctcaagcc gctgcaggtg gcgcgccaga gcgtcgtgcc ggccgtcaag
     5581 gagtccatgt gagtcccgaa caccaggcca gcgacatcct cgcccggctt gccgccgacg
     5641 gcgtcgaggc gacccgcctg tgcgccgact cgcgccaggt gctggcgggc gacatctttg
     5701 tcgcctatcc cggccaccgc gccgacggtc gccgctacat cgtcgatgcg gtgcacgccg
     5761 gtgcctcggc ggtgctgtgg gagcgcgagg gctatcagtg gaacgaggcc gtggacgtgc
     5821 cgaatgtacc ggtcttcgga ctggcccatc tgtccggcta catcgccgat gaattctacg
     5881 gccggccctc ccaggccctg tggacggtgg gcgtcaccgg cacgaatggc aagacctcgg
     5941 tcacccagtg gctggctcgc gcttacgccc agctggggcg tcggtgcgga ttgatcggca
     6001 ccctcggcat cggttttccc ggacagctca gcgcgacgct gaacaccacg ccggacgccc
     6061 tgacggttca tcgcgccctt gcggacatcc gcgatcaggg cggccaggcg gtggccatgg
     6121 aggtgtcgtc catcgggctc gatcaggctc gtttgaacgg cgtgcgcatc gacgtggccg
     6181 tgttcaccaa cctgacccgc gaccatctcg attaccacgg caccatggaa gcctacggtg
     6241 aagccaaggc ccgcctgttc gcgatgcctg gcttgcgcag cgccgtcctc aacctggacg
     6301 accgcttcgg catggagcag gcgcggagcc tcgcgggcag cggtgtcgag gtgatcggct
     6361 acaccgtgat cccatccaac cgggacgccg cacccgccca gcgtgtgctg gtggccgaaa
     6421 atctcaccat cacgccggtg ggcctgcgct tcaccgcgcg gtgcggcaat gtctcggccg
     6481 acctgttcgc caacgtggtg gggcgtttca acgcctccaa cctgctggcg gtgatcgcca
     6541 cgctgatggc ctcaggcatt cccttcgagg acgcgtgcaa ggtggccgag gggctcaccc
     6601 cgccccaggg ccgcatgcag accctcggcg gcgtcggcga tccgctggtg gtggtggatt
     6661 acgcccactc ccccgatgcc ctggagcagg tcctgcgggc cgtgcggccg acggcagagg
     6721 cgcgcggggg atcgctgacc tgcgtgttcg gttgcggcgg tgaccgggac cccggcaagc
     6781 gcccgctgat gggcgaggtg gcgcggcgtc tggccgaccg cctgatcctt accagcgaca
     6841 acccccgcac ggaagacccg cagcgcatcc tgcaggccat cgtggccggc gtgcagggcg
     6901 acgtggacat cattcccgat cgcgccgagg ccatccgtca cgcggtgatg cgtgccgctg
     6961 ccgacgacgt ggtcgtggtg gctggcaagg ggcacgaacc ttatcaggag atcaacggcg
     7021 tgcgcctgcc cttttccgac gcggaacaga cccgcgcggc gctggaagcc tggaacgatg
     7081 cccagggagg tctctcatga tggatctgca gggcgccgcg gtcctgaccc gcggtacggc
     7141 ggtgggcggg aacgtcgtgt tcgagtccgt gagcaccgac tcccgcgcgc tgcagagcgg
     7201 ccagctgttc gtcgccctgc gcggcgagcg cttcgacggc cacggcttcg tcgcccaggc
     7261 ggccagccag ggtgcggccg ccgcgatggt ggatcgtgcc tgggcggccg ccaatccggt
     7321 gccattgccg ctgctggtgg tggacgacac ccgcattggc ctcggccagc tcgccgccgg
     7381 ctggcgcgcg cgcttcgagc tgcccctgat cggcgtgacc ggcagcaacg gcaagaccac
     7441 cgtcaaggag atgtgtgcgg cgatcctgcg tgcgcgggcc cggcgcgagg ggcttgatcc
     7501 ggatctggcg gtgctcgcca cccgcggcaa cctgaacaac gacatcggcc tgcccctgat
     7561 gctgctgcgc ctgtccggca accaccgtgc cgcggtgatc gagatgggca tgaaccatcc
     7621 cggcgagatc ggctatctca cgcgtctggc cgcaccgacc gtggcgctcg tcaacaacgc
     7681 ccagcgtgcc cacctggaag gcatgggcag cgtcaatgaa gtggcgcggg aaaagggcgc
     7741 gatctacgaa ggcttgtctg ctgacggggt ggccgtggtg aacgcggacg atgccttcgc
     7801 cggctactgg aaggccctca acgcgggccg tcgggtgctg acctttggtc tgcggccggg
     7861 cgcggacgtg acggcccagg tcacgtccca tggatttgcc agtgaactga ccctcgcgtc
     7921 gccgaagggc gccgcgtcct ttcggctgca ggtgcccggt gaacacaacg tgcgcaatgc
     7981 cctggccgcg gcagccgcct gtctggcggc cggcgcgcgg cccgaggatg tggcggccgg
     8041 gttgggggcg ttcagtggca caccgggtcg gctgcacgtg cgggccggcg tcggcggcgc
     8101 gacggtgatc gacgacagct acaacgccaa tcccgattcc atgcgcgccg gcatcgccgt
     8161 gctggctgcc acgccgggtc gcaagatcct ggtggtgggg gacatgggcg agattggcga
     8221 tcagagtttc cagtaccacg acgagatcgg cggctacgcc aagagcgaag gcgtcgattt
     8281 cctctttgca ctgggtgaga tgagcgaggt ggccgcgcgc aacttcgggg acggcggccg
     8341 gcatttccag cgggtcgacg agctcgtcgc cgccgtgaag gccgagatga acagcaatac
     8401 ggtggtgctc gtcaagggct cgcgcttcat gaagatggag cgggtcgtgg acgccatcgc
     8461 ggagaaggct tgatgagggt ttgcggggaa tattcgtggg tggatgcgtg catgaccggt
     8521 ccggaggtgc gcgatgctgc ttgaactggc ccaatggctg agcaaggacg cccggttctt
     8581 caacgtgttc ggatacatca cgctgcgcac cgtgctggcg gccggtacgg cgctgttgct
     8641 gtcgctggtc ttcggtccgc cggtgatccg ctggctcgct gccaagaaga tcggccaggc
     8701 ggtgcgcgat gacggcccca agtcccacct caccaaggcc ggcacaccga ccatgggcgg
     8761 ggcgctgatc ctcatctcca tcgccgtcac caccttgctg tggggcgacc tgcgcaacaa
     8821 gtacgtgtgg gtggtgctgc tggtgaccct gggttttggc gcggtgggct gggtggatga
     8881 ctggcgcaag gtggtgtatc gcgatccgaa gggactggcg agccgctgga agtacttctg
     8941 gacgtcgatg gtcgcgctgg gtgcggccac cttcctgggt ctgaccgcca cgctgccggc
     9001 gcagatcgaa ctgatcgtgc ccttcttcaa ggcggtgacc tacccgctgg gggtcgtcgg
     9061 cttcatcgtg ctgagctact tcgtcatcaa cggcaccagc cactcggtca atctcacgga
     9121 cgggctcgac ggcctggcga tcatgcccac ggtgatggtc agcgcggcgc tggcgatctt
     9181 cgcctacgtg gccggccaca tgggcttctc caaatatctg ggcatgccct acatccccgg
     9241 tgcgggggaa ctcgccgtga tctgcggcgc catcgccggt gcgggactgg gcttcctgtg
     9301 gttcaacgcc tacccggcgg aagtcttcat gggcgacgtg ggcgccctgg ccctgggcgc
     9361 ggcaatgggc accatcgcgg tgatcgtgcg ccaggagatc gtgctcttca tcatgggcgg
     9421 cctcttcgtg gccgaggccc tgtcggtgat ggtgcaggtg atgtacttca agtacaccgg
     9481 cggcaagcgc atcttccgca tggcgccgct gcaccaccac tacgagctgg gcggctggaa
     9541 ggagacccag gtggtggtgc gcttctggat catcaccatc atgctcgtgc tgttcgggct
     9601 gtccaccctc aagctgcggt gaccggacat ggcggctgat ctgaccctca tcctcggtct
     9661 cggcgaatcc ggactggcca tggcccgctg gctggcccgc cagggtgtgg cgctgcgggt
     9721 ggcagattcc cgcgagaggc cgcccggcgt cgagcgcctg cgggctgagg cgcccgccgc
     9781 cgaggtggtc gcgggcccct tcagtgacgc gctcctcgaa ggcgtgacgc gcatcgccat
     9841 cagccccggc ctcgatcccc ggcagcccct cgtggcgacg gcccgcgccc gcggcctcac
     9901 cgtcgtcggc gagatggacc tcttcgtcca ggccctgcgc gatctgggtg tgcgcgacgc
     9961 gacgcggctc atcgccatca ccggcaccaa cggcaagacc accaccacca ctcttgcggg
    10021 cgagatggtc cgcgcctgcg gcgtggatgg cgtggtggcc ggcaacatca gcccggccgc
    10081 actggacgtg ctgatggcgc gcctggacgc cggcgaggcc ctgcccgccg tgtgggtgct
    10141 ggagctgtcc agcttccagc tggagacgca tccggcactc gaggcgatat cggccaccgt
    10201 gctcaacgtc accgatgacc acctcgaccg ttacgccggg ctcgacgact acgcggccac
    10261 caaggccgcg gtgttccagg gcgccggcgt gcaggtgctg aaccgggagg acgcccgggt
    10321 tgccgccatg gccgtgccgg ggcgacgggt gtacagcttc ggcgtcgacg cgccggccgg
    10381 gccggacgat ttcggtctgc gtaccgacgc cggcggcgag tggctggtgc gcggcgacga
    10441 gcgtctgctg gcgcgcgccg acctgcccct gccgggcaac cacaacatcg ccaacgcgct
    10501 ggcggctctg gccctgtgcg aggccgccgg cctgccgcgg gcgccgctgc tggccgcgct
    10561 gaaggccttc cgcggcctgc cccaccgggt cgagaaggtc gcccggcggg ccgacggcgt
    10621 ggtgtattac gacgactcca agggcaccaa cgtgggcgcc accgtggccg cgctggaagg
    10681 cctgcgtgcc cgcgtggtgc tgattgtcgg cggtgacggc aagggccagg acttttcgcc
    10741 gctcaaggac gccttcgccc gttatgcccg cgccgtggtg ctgatcggcc gtgacgcgcc
    10801 ggccattgaa gccgccgtgg ccggttgcgg cgtgcccctg atcccggccg ccgacatgga
    10861 cgccgcggtg ctcgccgccg acgcccgggc ccaggccggc gacgtggtgc tcctgtcgcc
    10921 cgcctgtgcc agtctcgaca tgttccgcaa ctacgcccat cgcgcccagg tcttcatcga
    10981 cgcggtgcgg cgcctgcccg gagtgcgccc cgcatgagcc tgaccgccag cctttccggc
    11041 ttcttctcgc gccacgccgg cgccgcgtcc cagtccgagg ccagccgcgc cgtgcgccgc
    11101 ctgatgcgcc ccctcggcca gccgcgggag ctcgatccgc tgctgatctg gtccgcggcg
    11161 gcgctgctgc tgctggggct gatcatggtg ttctcggcgt ccatcgccac ggccgagggc
    11221 agccgcagct tcagctacca gccggcctac ttcctgatcc gccacacggt tttccttgcc
    11281 atcggggtga tcgccggcgt ggtggtcttc caggtgccca tgcgtacctg gcaggagatc
    11341 gcgccgtggc tcttcatcgc cggtgtcgtg ctactgctgg tggtgctgat tccccacgtg
    11401 gggcgcgatg tgaacggcgc ccgccgctgg ctgcccctgg gggtggtcaa tctgcagccg
    11461 tccgagttga tgaagctctt cgccgcgctt tacgcggccg attacaccgt gcgcaagctg
    11521 ccctacatgg gcagcttcaa gaagggcttt gcgccgatgg ccgcggtcat cctcttcgtc
    11581 ggcttcctgc tcctcaagga gccggacttc ggcgcctttg tggtcatcac cgccattgcc
    11641 ttcggcatcc tcttcctggg cggcatcaat gtgcgcctgt tcgtgttgct ggcagtggtg
    11701 gccgcagtgg gcttcgtgtt gctgatcacc ttctcggaat accgccggca gcgcatcctg
    11761 ggcttcatgg atccctggca ggacgcctac ggcaagggct atcagctgtc ccacgcgctg
    11821 atcgccttcg ggcgcgggga gtggtttggc gtggggctgg gaggcagcat cgagaagctc
    11881 ttctacctgc ccgaagccca taccgacttc ctgctggcgg tgatcgccga ggaactgggt
    11941 ttcgccgggg tgttcaccgt ggtggtcctg ttcggaatcc tgatccaccg ggccttcgca
    12001 atcggcaagc aggccctggt gctggagcgc acctatgcgg ccctggtggc ccagggcatc
    12061 ggcatctgga tcggcgtgca gtccttcatc aacatgggcg tcaatatggg cttgctgccc
    12121 accaagggcc tgaccctgcc cctcatgagc ttcggcggtt ctggcatcct ggccaactgc
    12181 gtggcgctcg cgatcctgct gcggatcgac tgggaaaacc gccagatcac ccgcggaggc
    12241 aacgcgtgat gcccaccctg atggtcatgg ccggcggcac gggcggacac atctaccccg
    12301 gcctggcggt ggccgacgcc ctgcgtgcgc ggggctggaa ggtcgtctgg atgggcaacc
    12361 cggacggcat ggaggcgcgc atcgtgccgg ccagtggcta cgacatggcg tgggtgcgct
    12421 tcaccgcgct gcgcggcaag ggcctgctgc gcaagctgct gctgcctttc aacctgctgc
    12481 gtggcttcgc ccaggcgttg gcccagctcc gccgtgtgcg gcccgacgtg gtgctgggca
    12541 tgggtggata cgtcaccttc cccggcggca tgatggccgc gctcatcggc cggccggtgg
    12601 tggtgcacga acagaattcg gtcgccgggc tggccaaccg ggtgctggcg ggcgtcgccg
    12661 acaaggtcat gaccggtttc ccggacgtgc tgcgcaaggg cgagtgggcg ggcaacccgg
    12721 ttcgcgccga catcgccgca gtggcgccac cggcccagcg ctttgccggt cgcagcggcc
    12781 cgctgcgggt gctggtggtg ggcggcagcc tcggcgccgc ggccctcaac gacgcggtgc
    12841 ccaaggccct ggcgctgatc ccgccggccg agcgtccgca ggtgacccat caggccggca
    12901 cgcgccagat cgacgccctg cgcgccgcct accgggcggc tggtgtcgag ggcgagctgc
    12961 tgcccttcat cgacgacatg gccgagcgct acgctgcagc cgacctggtg atctgccgcg
    13021 ccggcgccct caccgtggcg gagctggccg cggccggcgt ggccagcgtg ctggtgccct
    13081 ttcctcacgc ggtggacgat catcagaccg gcaatgcccg tttcctcgcc gatgccgggg
    13141 cggcggtgct gctgccccag accgaacttg acccggcgcg cctggccgcg ctgctgggca
    13201 gcctgaatcg cgagcgcctg ctggaaatgg cggagaaggc gcgggcgcgg gcccgcccgg
    13261 acgccacctc ccgcgtcgcc gacgtatgcg ccggcctcgc tgcgtaaagg aagaatcgtg
    13321 aaacacaaag tcaagagcat ccacttcgtc ggcattggcg gtgccggcat gagcggcatc
    13381 gccgaagtgc tggtcaacct gggctatcag gtgagcggct cggacctggg cgacaacgct
    13441 gcgacgcgcc gtctcaagga actcggtgtc cgcgtgatgc agggccacgc cgccgagaac
    13501 gtgcaggacg cggacgctgt ggtgacctcc accgccgtca agggcgacaa cccggaagtc
    13561 atcgccgccc gcgcgcgcca gatccccgtt gtgccccgcg cccagatgct ggccgaactg
    13621 atgcgcctca agcaaggcat cgccattgcc ggcacccacg gcaagaccac caccacctcg
    13681 ctggtggcga gcatcctggc cgaaggcggg ctggatccga ccttcgtcat cggcggcaag
    13741 ctcaacgccg ccggcgccaa tgcgcgcctg ggcaaggggg atttcctggt ggccgaggcg
    13801 gacgaatccg acgcatcctt cctgcttctc accccggtga tctcggtcgt caccaacatc
    13861 gacgccgatc acatggaaac ctacgggcac gacttcgccc ggctcaagca gtccttcgtg
    13921 gatttcctgc agcacctgcc tttctacggc gtggcggtgc tgtgcgacga cgacaagcac
    13981 gtgcgctcca tcatgccgct cgtgtccaag cagatcgtgc gctacggctt ctccgaatcc
    14041 gccaacgtgc gcgccgagaa catccacgcc gaagacggcc gcatgcgctt cgacgtggtg
    14101 cgggtaaatg gcaccaccag ccgcatctcc gtggtgctca acctgccggg ccgtcacaac
    14161 gtgctcaacg ccctggccgc catcgccgtg gccaccgagg tgggcgtctc cgacgaggcc
    14221 atcgtcaagg cgctggcgga cttcaagggc gtcggccggc gcttccagcg ttatggcgag
    14281 gtggcgctgc cggcgggcgg ggcggccacg gtcattgatg actacggcca ccacccggtc
    14341 gagatggccg ccaccctcgc cgcggcgcgc ggcgcatttc ccgggaggcg tctggtgctg
    14401 gccttccagc cccaccgcta cacgcggacg cgggactgtt tcgaggattt cgtgaaggtg
    14461 ctgtccaccg ccgatgcgct gctgctgggc gaggtgtatg ccgccggcga ggcgcccatc
    14521 gtggcggccg acggacgctc cctggcccgc gccctgcgtg tggcggggcg catcgaaccg
    14581 gtgttcgtgg aacacatcga ggacatgccg caggccatcc tggccgcggc gcaggacggc
    14641 gacgtggtga tcaccatggg cgccggcagc atcggcgctg tgccgggcaa actggtgaat
    14701 ggagaggtct gagggtggac gcagtgcgca gtcaattcgg aaaggtcgcc gtgctgctgg
    14761 gcggctcgtc ggcggagcgg gagatctccc tgatgtcggg caaggcggtg ctggcggccc
    14821 tgcaaggggt aggcgtagac gcccacgcct tcgatcccgc cgagaccgac ctgcacatcc
    14881 tcaaggagga aggctacgag cgcgccttca tcgccctgca cggccgcggc ggcgaggacg
    14941 gcacggtgca gggggccctg gaactgatgg gcattcccta cactggcagc ggcgtgatgg
    15001 cctcggccat cgccatggac aagtggcgca ccaagctcgt gtggctggcc agcggcctgc
    15061 ccactccgcg cttcgcgatc ctgaccgccg acagcgactg ggatgccgtg gtggcgcagc
    15121 tgggcctgcc gatcttcgtc aagccggtgc atgagggctc cagcatgggc gccaccaagg
    15181 tcaccgaggc ggcccagctc aagggcgcct gggagcttgc cgcgcagtac gactcactgg
    15241 tgctggccga ggaattcgtg tccggccagg aactcaccgc ccccttcctg ggggatcggg
    15301 cgctgccgct ggtgcgcatc gtcgcgccgg atggcaatta cgactatcag cacaagtact
    15361 tcaccgacga cacccagtat ttctgcccga gcggcctgcc ggaggccctg gaaaaggaca
    15421 tccaggccac catcgtgcgt tgcgccaagg tgctgggctg ccgcggctgg ggccgtgccg
    15481 acctgatcct gcaggacgac ggccgctaca cgctgctgga gatcaacacc gcgccgggca
    15541 tgaccaacca ttctctggtc cccatgtcgg cgcgcgtgtc cggactgtcc ttcgaggccc
    15601 tgtgtctcga gatcctggcg ggagcccgcc ttggttgatc gccgcgccac ctccaacgtg
    15661 ccgcctgaag ccggcgccaa gccgtcgttg ccgcggcggc cgcggcctgc ggcgcgcgcg
    15721 cagaagcgcg acggaggagg taacggcctg tgggatcgcc cccagctcct caacctgatc
    15781 gccgatctgc tgctggtggc cggtgcggcc ggcctcggct atgcggccgt ggcgggcctg
    15841 tcgcgcctgc cggccttccc gctgaaggaa gtggtcgtca ccacgcccct cgcccaggtg
    15901 accaccgaac agctcgaata cgccgcgcgt tcgtccctca agggcaactt cttcacggtc
    15961 aatctggagg aggtgcgcaa cgccttcgaa aagctgccct gggtgcgacg cgcagacgtg
    16021 cgccggcgct ggccgggcgg cgtcgaggtg cgcctcgagg agcacgtggc ctccgcctac
    16081 tggcgtgttg gcgacacggg cgacatgcgc ctggtgaatc gctacggcga gatcttctct
    16141 gccgcgagca acgcgccgat gccgatcttc agcgggccgg agggctcgtc gggcgtcctc
    16201 ctggccaagt ttgacgagtt cagccacaag ctcggtcccc tgggccgcgg actggtgggt
    16261 gtctccctgt cagcccgtga ggcgtggcag ctcaagatgg cggatggact caccatcgaa
    16321 ctgggccacg accaggccaa ggccccgctg gacgagcgga tcgaccgctt cgtgcgttat
    16381 tacccccggg ccaaggccca gttgaacatg aacgtggcgg tggtggacct ccgctacccc
    16441 aatggttttg ccctgcgcgc ggtgcccgtg gccgcgccgg ccggcaagca agacagcaaa
    16501 gggaagtgat gagcaaagag tacaaggaac tgatcgtcgg cctcgacatc ggcacctcca
    16561 agatcacctg cgtggtcgcc gaactccggc ccgacggccg cctggacgtg atcgggctcg
    16621 gcacccagcc ctcgtcgggg ctcaagaagg gcgtggtggt gaatatcgaa tccaccgtcg
    16681 atgccatctc ccgcgtgatc caggaagtgg agctgatggc cgactgcaag atccgcgatg
    16741 tctacacggg catcgccggc agtcacatcc gcagcttcaa ctccaacggc atggtggcga
    16801 tcaaggataa ggaagtgtcg cccatggacg tggaacgggt catcgaaacc gcccgcgcga
    16861 tgcctctgcc ggccgatcag cagattcttc acatcctgac ccaggaattc atcatcgacg
    16921 gccaggacgg tgtgcgcgag cccctcggca tgagcggcgt gcgcctggaa gtgaaggtgc
    16981 acatcgtcac cggcgcggtg tcggccgctc agaacatcgt caagtgcgtg cgccgctgtg
    17041 gcctggaagt gctcgatctg gtgctgcagc cgctggcgtc cagctacgcc accctgtcgg
    17101 aggacgagaa ggaactgggc gtgtgcctgg tggatatcgg cggcgggact accgacctgg
    17161 cggtgttcac ccagggcgct atccgccaca ccgcggtgat tccctacgcc ggcgaccaga
    17221 tcaccaacga tatcgccatg gccctgcgta cgccgaccgc cgaagccgag gagatcaaga
    17281 tccgtcatgg cgtggccatg cacaccctga ccgacccggc cgagatgatc gaagtgcccg
    17341 gcgtcggcga ccgtccgccg cgcaagctct cgcgtcaggc cctggccgac gtgatcgagc
    17401 cgcgtgtgtc ggagatctac gaactggtgc aggccgaact gcggcgcagc gggtacgagg
    17461 aactgctctc gtcgggcatc gttctgaccg gcggttcggc ggtcatgcag ggcatggtcg
    17521 agcttggtga ggaggtgttc cacatgcccg tgcgcatcgg ataccccaac tacagcggtg
    17581 cgctggcgga ggtcgtctgc gagccgcgtt atgcaaacgc gatgggcttg gtgctggaag
    17641 gcgccgccca gaaaaggcgg ggcatccagg ctcgccagac gcgcaatgtg gggcaggtgt
    17701 tctcgcggat gcggtcctgg ttcgagcgga atttttgagg gtcgccggag acatccatcc
    17761 ggacgaataa agttgcgccg gtacccttat ttagttgtaa actcttccgt taatactata
    17821 tataggtcaa ccggtgcgca gcgcgcaggc agtacagatt ctcttggagg ctatgtaaat
    17881 gtttgaaatc gttgagaagg cgtcgggtgg cacgagcatc aaggtgatcg gcatcggcgg
    17941 ggcgggcggc aacgcggtcg accacatgat ccgtcagggc gtgcagggcg tggatttcat
    18001 ctgcgccaat acggatgccc aggcgctgac ccgctgtctt gcgccggtca aggttcagct
    18061 cggcaagacc ggtctcggtg ccggatccaa gcccgaagcc ggtcgcgccg cggcccagga
    18121 atcccgcgag cagatcgccg ctgcgctcga aggcgcccac atgtgcttca tcaccgccgg
    18181 catgggcggc ggcaccggca ccggcgcagc gcccgtggtg gccgaggtcg ccaaggaaat
    18241 gggcgtgctg acggtcgccg tggtgaccaa gcccttcgat ttcgagaacc gtctgcgggt
    18301 ggccgaaagc ggcgtggaag agctctcccg ccaggtggac tcgctgatcg tcgtgctcaa
    18361 cgacaagctc ctcgaagtct atggcgacga cgccggcttc gaagactgct tccgctccgc
    18421 cgataacgtg ctgaccagtg cggtgggcgg catcgccgag atcatcaatg tgcccggcct
    18481 ggtgaatgtg gacttccagg atgtgcgtac cgccatggcc gagatgggcc gtgccatgat
    18541 gggctccgcc gaagccgccg gcctcgaccg tgcccgcatc gccgcagagc aggccgccgt
    18601 gtcgccgctg ctggaaggca ccgagctctc cggcgcccgc tgcgtgctgg tgaatatcac
    18661 cgccagccgt tccctcaaga tgtccgaagt gcgggacgcc gtgaagaccg tgcaggcctt
    18721 cgccgcgccg gaagccttcg tcaagtacgg caccgtgttc gaagagaata tggaagaccg
    18781 catccgcgtc acggtcgtcg ccaccggtct cggtgccgtg cgggcggctg tggcccgcaa
    18841 gccggagatg caggtgctgc ggaccggtac cgacaacatc ggcgtcgaag tggattacgg
    18901 caatctggaa ctgccggcgg tgatccgccg cacgtcccgc accacggtcg aggcgatgtc
    18961 cgccagcggc gtgtccaccc tggacatccc ggcgttcctg cgcaagcagg ccgactgagc
    19021 acccgccggt ctgatggccg gacgacagca gcgcatgcgc ggacgccgcc tcccgtccgt
    19081 tcatgcgctg tttgtctttg gagggtcggc gtggcattgc gcagtgcagc acggtgggcg
    19141 tgttaaaatt cagacctctg catccgaggc gcgatcatgc tcaagcagcg caccctgaaa
    19201 tccgcgatca gcaccaccgg tgtcggcctt cacagcggca acaaggtgac gctcagtctg
    19261 cggcccgctc cgccggatac cggcgtggtc ttccatcggg tcgatctcga tccgcccgtg
    19321 gatctgccgg ccgatccgta cgccgtgtgc gatacgcgca tgtgctcggg tctgcagaag
    19381 ggaacggcca aggtcaatac cgtcgagcac ctcatgtcgg cgctggccgg ccttggggtg
    19441 gataacgtcc atatcgacgt ggacgccgcc gagattccca ttctcgacgg cagcgccggg
    19501 cccttcgtgt tcctgatcca gtcggccggc atcgaggaac agaacacccc caagcgcttt
    19561 atgcgggtga tcaagcccgt ggagtatcgt gagggcgaca agtgggtcag gctggagccc
    19621 tacgatggct acaggctgag cttctccatc ctcttcaacc acccggccat cgaccgtacc
    19681 ggcacctctg tcaccgtgga ttttgccgag cacgcctaca cccgtgaggt ggcgcgggcg
    19741 cgcaccttcg gcttcatgca ggacgtggag ttcatgcgtg ccaacggcct gggtctgggc
    19801 ggcagcctgg acaacgccat cgtgatggat gaataccgcg tgctcaatgc cgatggcctg
    19861 cgctacgcgg atgaattcgt caagcacaag gtgctcgacg ccatcggcga tctctacctg
    19921 gtcgggcacc cgctgctggc tgcctacagc gcctacaagt cgggccacgc cctcaacaac
    19981 cagatcctgc gggtgctgct cgaagaccgc agcgcgtggg agatcgtcac cttcgaggaa
    20041 gacgcccgca cgccgccagc ggtcgcccat ctgttcgatc tggcgctcgc ctgatcggcg
    20101 ccgcggccgt gctgctcctg cgactgatcg cggtgctggt ggccatcggc atcggcgcct
    20161 cggcctttgc ctacctgttc acccgcgacc gccgctacct cgcgttcgcc ctgcggctgg
    20221 cgcgctatgc gctgatcgtt gcgctggtgt tcctcgccct gttggccctg gagcgggtcc
    20281 tcgcgccggc ctttggctga ggctcatccc gccgagcgga cgaagcggcg cagcgcctgg
    20341 gccagcggcg aatcggccgg caggcgcccg gccagctcat ccagccctgc gcgggtccca
    20401 tcgctcaccg aacgatgcac cggtgcggcc gccgggcggg gcgattccgg ggccgccacc
    20461 ttcaccttga tgtcgcgcag ggctacgccg gcgcgggcaa agtctgccag caggccgggg
    20521 atcgcctggc gcagcttgac cgcgacggcg gcgccgcggg tgtggatcac cagcacctcg
    20581 tccttcaggt tggcgacctg gcaactcgcg gccagatagt ccggcagcac atcgctgagg
    20641 gtgcgctgca ggcgcagcag gcgcgcggcg tgggcgtgga ggcgggcgag ggtgtcgccg
    20701 cggtcgaggt aggcgtcgag ggcgcgggcc atggtctggg gcttggaatc ggcagaagcg
    20761 cccccatcct aggcgggacg ctgcgcggcg acaagcccgc gctgccgccc ccaggcgtca
    20821 cggtcccgtc ccggtgccat gatagaatct ccgttttgcc gcgtttttcc ttccaaatca
    20881 aatgatcgca ggccttctca agaagatttt cggtagccgc aacgaccggc tgattcgcca
    20941 gtatgcccag aaggtgcgtg tcatcaacgc gctggagccc acggtccagg cgctctcgga
    21001 cgaggcgctg cgcggcaaga cggccgagtt ccgccagcgt ctggccaatg gcgaagccct
    21061 cgatgcgttg ctgccggaag cctttgccgt ggtccgggag gccggcaagc gggtgcatgg
    21121 catgcgccat ttcgatgttc agctcatcgg cggcatggtc ctgcatgacg gcaagatcgc
    21181 cgaaatgcgc acgggtgaag gcaagacgct gaccgccacg ctgccggtct acctcaacgc
    21241 cctggccggc aagggcgtgc atgtgatcac cgtcaacgac tatctggcga gccgcgactc
    21301 cgactggatg gggcgcattt accgcttcct gggcatgtcc gtgggctgca acctgtccca
    21361 gatgccccac gaattgaaga aggcggcgta cgaggcggac atcacctacg gcaccaacaa
    21421 cgaattcggc ttcgactacc tgcgtgacaa catggtgtac agcctggagg agcgcgtcca
    21481 gcgcggcctc cactacgcca tcgtggacga ggtggactcc atcctcatcg acgaggcgcg
    21541 cacgccgctg atcatctccg gccaggccga ggatcacacc gagctttaca ttcgcatgaa
    21601 cgcggtgccg ccgctcctga cccgtcagga aggcgagggc gaggacatca cccagcccgg
    21661 cgactacacc gtggacctca aggcccactc cgtgctgctc accgagcagg gccacgagca
    21721 cgccgaggag atcctcggtc gcctgggcct gctgccggaa ggggccagcc tctacgatcc
    21781 ggcccacatc ctgctgatcc atcaccttta cgccgcgctg cgcgcccacg ccctgtacca
    21841 caaggaccag cattacgtgg tgcagaacgg cgaagtggtg atcgtggacg aattcaccgg
    21901 ccgcctgatg cccggccggc gctggtcgga gggcctgcac caggccgtcg aggccaagga
    21961 aggcgcgcgc atccaggccg agaaccagac gctggcctcc atcaccttcc agaactactt
    22021 ccgcatgtac ggcaagctgt ccggcatgac cggcacggcc gacaccgaag cctacgaatt
    22081 ccaccagatc tacggtctcg agaccgtcgt cgtgcccacc gcccgtccca tggtccgcaa
    22141 ggaccagaac gacaaggtgt atcgcacgat gaaggagaag cacgacgcga tcctggcgga
    22201 catccaggac tgcgtgcagc gcggccagcc cgtgctggtg ggcaccacct ccatcgagaa
    22261 ttccgagttc ctgtccgacc tgctgacccg cgccaagctg ccccataacg tgctcaacgc
    22321 caagcagcac gcccgcgagg cggagatcgt cgccgaggcg ggccgtcccg gggtgatcac
    22381 catcgccacc aacatggcgg ggcggggtac cgacatcgtg ctgggcggta acgtggacaa
    22441 gcccatcgcc ctgctgcgcg aggacgagaa cattccggat gccgacaagg aggcgcagat
    22501 ctcccggctg cgcgaggaat ggaaggtccg ccacgagcag gtgctgcagg ccggcggcct
    22561 caagatcatc ggcaccgaac gccacgagtc gcggcgcatc gacaaccagc tgcggggtcg
    22621 ctccggccgc cagggcgacc cgggcgagag ccgcttctac ctgtccctcg aagatccgct
    22681 gatgaagatc ttcgccggcg agcgcctcaa cgccatcatg gttcgcctca agatgcccga
    22741 aggcgaggcg atcgaggccg gcatggtgac ccgttcgctg gaaaccgccc agcgcaaggt
    22801 cgaacagcgc aacttcgaca tccgcaagca actcctcgaa tacgacgacg tggccaatga
    22861 ccagcgcaag gtcatctacc agcagcgcaa cgagctgctc gaatccaccg acatcaccga
    22921 gaccatcacc gccatgcgcg aaggcgtgct gcacgatctg ctgcgcacct acgtgcccgc
    22981 cgagtccgtc gaggagcaat gggacatccc cgccctcgaa ctggcgctgc agtccgaaaa
    23041 ccagctcaag ctgccggtgg gcgactggct caaggccgaa cccgacctga ccgacgatga
    23101 cctgctgcag cgcatcctgg aggccggaaa tgcggcctac gcggacaaga tcagcctggt
    23161 cgatgcggcg gcctggcatg gcttcgagcg caacgtgatg ctgcagagcc tcgacaccca
    23221 ctggcgcgaa cacctctcgg cgctggacca cctgcgccag ggcatccacc tgcgcggcta
    23281 cgcccagaag aatcccaagc aggaatacaa gcgcgaggcc ttcgaactct tcgagaacct
    23341 cctcagcgtc gtccgcgtgg aggtcaccaa gctgctcatg accgtgcaga tccgctccga
    23401 ggcccagctc gacgaggccg aggcgccgcc cgagctggaa aacgttcagt accaccacgc
    23461 cgactacgac gaggccctgg ccaccgccaa tccggacgcc ggcgtcgcgc agcctgctgt
    23521 caatctgacg cccaaggtcg gtcgcaacga tccctgcccc tgcgggtcgg gcaagaagta
    23581 caagcactgt cacggcaagt tgaactgacg ctacgcgtag gtgcagacat ccggggccgg
    23641 caagtgccgg ccctgacttt ttggcggggg cgcctcaagc ccgtgcggca gtctgccgtt
    23701 gaaggggtga ggccccgcca tgacccttcc cgcccatcct ctcgacctga ccggctggat
    23761 cgaacgcatc cgcggtcgtg acatgcctgt cttcggtcgt accgtcgaat cgctgcgcac
    23821 cctgctcagc gatgatcggg tctcggcctc gaccctcgcc caggtcatcc tcaaggatgc
    23881 gccgatgacg gccaaggtgc tacgcctcgc caacagcgcc ttcttcaaca cctcccgcca
    23941 gaccgtcagc accgtgtcgc gggccatcgt cgtcctcggg ttcaatcccg tcgcgcagat
    24001 cgccctgtcc gtctccctca tcgatgcact gctctccggc ggcgtgcgcg ggcgtgccga
    24061 agccgagatg gcgcgcagct ttcacgccgc cgtgcaggca cgctgggcgg cggccaaacg
    24121 gggcgaaggg cacggcgagg aggtcttcat cgcggccctg ctgacccgcg tgggcgagat
    24181 ggccttctgg tgtttcggcg gtgatcaggc gcgcagcctg gatggcctcc tggcccaggg
    24241 gcggatgcgc gaagaggatg cccagcaggt ggtgctgggc tttccgctgc ggcatctgtc
    24301 cgccggtctg gtgcgcgaat ggcacctggg cgcgctggcc caggcggtgc tggaaggtca
    24361 gtcccgcggt cggaacggtg aacaggcggt gcttctcggt catcggcttg cccgcgcgtc
    24421 ggagcagggg tgggatgcgc cccctgcgcg gcaggccgtg caggcccttg ccgattatct
    24481 ggagctgcca ttcgccgccg tgctcgatgc ggctcgcgcc aatgccgccg aggcggcccg
    24541 tatcgccagt tccttcgggg cgcctgctcc acctgcgggc gctgcggcgt ctgcaggcag
    24601 cgggccggct caggccgacc cggcagcgcc gccagtcggt gtggccgatc cccatctgca
    24661 actgcacatc ctgcacgacg tggcggccat gaccctcgcc caggcccccc tcgacgacat
    24721 cctgcagatg gtgctggaag gcctgtaccg gggggtcggc tgcgaacggg tactggtggc
    24781 gcgactcagt gccgaccgca gtgaattgca gggacgcgtc gccattggat ccggtgccga
    24841 ggccctggcg gcaagcttcg tgttttccat ggacggcgag cccgacgacc tgctcgatgc
    24901 ggccatcgac agtgcgcagg gctgctggct gacgcccagc cgcgcggccc agccgggcgc
    24961 cgagcgcctg ctcaaggtgg ccggcagccg acacgcctac ctggcaccct tcgggaccgg
    25021 cagtcgacag gtgggcgtgt tctacgccga cggccacggg cgggccctgg atgaagccgg
    25081 gtggcacgct ttccagcatt tcgtgttgca ggccagcctg gccgtgggcc tgtccctgca
    25141 agggcagtag accgggcgga tcggcggttg gcgcggcagg cgtgcctggc actgtccgca
    25201 cgctgcatga aaatcccggt cgagggatgc gcctgtcacg cgctccggat tatcatccca
    25261 agctttgtga ccgtccgctt ccggagtact gccatgcccg tcctgctctc gacccccgat
    25321 cccgtctgcc tgctgcccgt gcccggcgtc cgcctgggcg tggccgaagc cggcatccgc
    25381 aaggccaacc ggcgggatct caccctcatc gagctggccc ccggcagccg cgtggcgggc
    25441 gtgttcacca ccaaccgctt ctgcgccgca ccggttcagg tgtgcaaggc ccatctcccg
    25501 gcgggcacca tccgcgcgct ggtgatcaac accggcatcg ccaacgcggg cacaggcgag
    25561 ccggggctcg cggcggcgcg cgccacctgc gaggccgttg cggcgcagct cggtctcgct
    25621 gcggaccagg tgctgccgtt ctccaccggg gtgattctcg aaccgctgcc cgtggagcgc
    25681 ctcgtcgccg gtttgccggc ggcccgcgcc gccctcaggg cggacggctg gttcgacgcg
    25741 gcccacgcca tcatgaccac cgacaccctc gccaaggccg tgtcgcgcca ggtgcaggtg
    25801 ggtggcaaaa ccatcaccct gacggggatg tccaagggcg ccggcatgat caagcccaac
    25861 atggccacca tgctgggctt cctcgccacc gacgcggacg tggcccagcc cgtgctggac
    25921 gcgttgctca aggaggtgac cgatctcagc ttcaacagca tcaccgtgga tggcgacacc
    25981 tccaccaacg acagctacat cctcatcgcc accggccagg ccggcaacgg cacgcttacc
    26041 gacacagcct cggccgacta cgccgcgttc cgcgaagcgg tgctggacgt gtccatccgt
    26101 ctggcccagg ccatcgtgcg cgacggtgaa ggcgcgacca agttcatgac cctgcagatc
    26161 ggcggcgggc gggatcgcga ggaatgccgc aaggtgggct atgccatcgg tcactccccg
    26221 ctggtgaaga ctgccttctt cgcctccgac cccaacctgg ggcgcatcct ggcggcgatc
    26281 ggctacgccg gcatcgccga cctggatgtg gcggccctgc gggtgtggct gggcagccag
    26341 ggtgaagagg tgctggtggc cgaacagggc ggccgcgcgg cgagctaccg ggaagaggac
    26401 ggtgcccgca tcatgcaggc ggcggagatc acggtgcggg tggatctggc ccgtggcgat
    26461 gcggcggcca ccctgtggac ctgcgacttc tcctacgact acgtgaagat caacgccgac
    26521 taccgttcct gagcccggag gccgccccgt gaagacgagt ccgctggatt acgtgctgag
    26581 cacgatcgcc atcaccctga tcctgctgct cgcggtggcc ggcgtgtggg cgctgcgccc
    26641 gctcacgacg gccgcgctgg cggactatca cgtggtggcg gacttcctgc tgctgctgct
    26701 tttctacggc ctgctgtcag ccgtcgcggt gcgggtgctg ctgcgtgtgc agcccatcgc
    26761 gccgggcgag tacgacggcg attcgccggt gtttacgttc tggaagctca tcaccatcgt
    26821 ctatcgcctg ggccagggcg cgctgctgcc tttcaccacg gtgttcacgc gaccggtggt
    26881 ggaggccctg tatggcgccc gggtgggggc ggacgtggcc atcggcggca cgatcgacga
    26941 cccctaccag gtggagatcg gaccgcgggc ggtgatcggg cacaacgcgc tggtgtcggg
    27001 caacgtgctc tacggcggca agctcacctg cggcaaggtg atcatcggcg cgggcgccac
    27061 catcggcgtc aattcggtgg tgctgcccgg ttccgagatc ggcgagaacg ccgtgttgat
    27121 gggcggttcc tacctcatgc ccggcaccaa ggtgccggca ggcgagacct ggcgcggtaa
    27181 cccggcgcgc aagtggatgt aggccgggtg gtgccagcgg tcgggcgcag cggggcgcgg
    27241 acggtaaaga ccgccgcgtc ccgtgctatt cagcgcagcg gaaagcccag gttgcgcagc
    27301 gcctctgcca gacggtcacg gccggtgagg gatgcggcgt cacggatgcg cttggccgaa
    27361 tggttcgacc aggtgccgcg caccttcatc ccctgggcgg cgtagaaggc cgccatggcc
    27421 tggtcgtagg tctcgaggcc ggcttcctgg gccggcaggg agtaggtttc ctgatgcagg
    27481 accacggact gcggcgggcg cggcttgact gcggcgggcg ccgccgggtc cggcatgccg
    27541 acgcacaggc cgaagacggc caccacccgc ggtggcagct gaagttccgc ggccacgtct
    27601 tccgggcggt tgcgcatggc gccgatgtat accgtgccca ggccgaggga ttcggcggcg
    27661 gccacggcgt tctgggcggc aagggcggcg tcgatgacgg ccacctcgaa catttccagg
    27721 tagtccagtg cggcggaggt ttctccgacg cgccgggcga cctgttcgag gcgggccagg
    27781 tcggccagcc agacgagcag cagcggggcc tggcggatgt gggcctggtc gccggcgtag
    27841 tcggccagac ggaccttgcg ggcggcatcg cgtactgcga ccacgctcca ggcatgcagg
    27901 ttggacgagc tggcagcgga ctgggcggcg gccacgatgg ccgccagcgt gtcgtcgctg
    27961 accgggtccg gcaggtaggc gcggaccgag gcgtgggcca gcaggtggtc gatgacaggg
    28021 gaggctgcgg ccggaacggc gacgtccggg ttgccgtagc gggcctggta gagggcggag
    28081 cgggcgttca tggggatcct ggctggaaag acggggacgg gcaccgcccg tcccgggccg
    28141 gattctagcg ccaaaggtcg cgtcgggtgt ggcggccccc gcccatcgtg cgcagggtgc
    28201 ttactgggcg cgcggtttgc gggtgcggcg cggggccggt gcggctggcg gggcttcgtc
    28261 ggccgtgctg gcgagggctt cggcgatctg acggacctgt tcttccatgg cgccgacgcc
    28321 gcgggtggcg ctgtcggtca gctcattcag gcaggtctcg gcctgttcga gggccgtgcg
    28381 cacgtggctg atggcgctgc cggtttctgc gtccgcatgt tgaatggcgt gggccattcc
    28441 gcgcttgagg gcgaggtcga agatgccgac ctgcttgcgg gcgatctgca gggtggtctg
    28501 gtgtgcctgg gcgataatgg caaagctgtc gcgcacgagt tcggcggagt cgctgcgcca
    28561 ggcatcgagg ctgccgatgg acaggggtag gctggcgccc ggttgggcga gctgactcag
    28621 atggcgctcg ctttcgtcga gcatctgacg gccggcccgc aggatcagct cggagagctt
    28681 gccggtggcg tcgatccagg cctgggatgc ggcctcgatg gcgtgcaggg tgtccaggcg
    28741 gttctggtgg atttcatcgg gggagaggta catggcggac tccgtgtggc gattgtgctg
    28801 tgcagcataa cgcctcgcgg ttgcatcggg gtgacaaatc gtacgcatcc cgcgcgatgt
    28861 cgcgggcgca gacgcgtcag caggcgctgc cggctggcag gtcggggctg ccggacgtgt
    28921 agcccgcgtc gatctgttcg ttcaccaccc gccacatggg tttcatgaaa aagcgcagtc
    28981 cgccgatgat gcccttcacg tccatcatgg atggaatgcg cgtgccacgg tgttcgaccg
    29041 gaccgccgat ctggcggatc ttgacgccga gtttggagaa gtgatcggcg aggcgcggtt
    29101 cggtgagcac gaagagggtc tcgacgcctt cccgctgggc gatggccacc accgcgagat
    29161 acaggctgat ggggatgtag gggaaacgcg gctgggcggc ggtgccgaag tcgctgtcgc
    29221 tgagggcgac ttcgctgcgt gcttcccctt tacgacggcg gtacaggccg cgcactgcga
    29281 gacgtgagac ctcggcgatg gcgccccggg gctccttggc cgggtcgatg atgcggcgat
    29341 cgagggtggc cgcgcaggtg cgctcgaagg gcagcgggta ggccggatcc tccggtcgtg
    29401 ccaggacgag gcggttgcac ccgaccaggg tgtggggttc ggtcgaggtg cgcagcaggc
    29461 aatggcggct gtggcggtcg tactcgtcgg tctcgcgccg attggcgcgg acgggctcga
    29521 agcccagttc ctcgcagtac acctcgtggc gaatggcgaa aacgtcgtcg cggccagcgt
    29581 tgtcgaaggc cgcgctgatc tggaaatact tgcggaatcc gtcgcccagg ttcagcgtca
    29641 ggttgtcgaa gaaggccatg gctgcgtact cggatcgaaa gaggtcagac gttctgccgc
    29701 agggcatcca ccaccggcag gcgggagacc ttgtaggcgg ggaacaggga ggccagcacc
    29761 gtcgcgacaa agccgaccag gaaggcgccg gcgagggtac ccggcaccac ccggatgaat
    29821 gcggtgtagc ccacgttggc attgggcggc ggcggcatcg ggattccaat ggccgagatg
    29881 atcagcgctg cgatgacacc cagcaccacg ccggccaggg cgccgcccag gcccaccagg
    29941 gccgactcga gcacgacgag gcggacgacg tcccgggccc gattgcccag ggcctgcatc
    30001 gtgccgaatt cgccctggcg ctcgtagacg gtcatgttga cgctgttggt gacggacagc
    30061 agcaccatca gcaggatgat cagccgcagc acgccgaact gggtgtcgta gagctggacc
    30121 gacttgtcgt agaaatccga cagggtgcgc cagctgcgga cgtcgtaagg gctgtccgcg
    30181 agctcgacgc tggcgcgttt caggacggca tccgtgtcgt cggtgcggtg cagggcgacg
    30241 accacctgat tgacgccgcg ggtctgcagc agatcctgcc cggcattcag cggaatgcgc
    30301 acggcacggg cgtcgaattc cttcgagaaa ctctggaaga tgccaacgac gcggaagtcg
    30361 tgggtgttca tggcgccccc ggccgaactg gtcacgaggg tcagggcgtc gcccggtttg
    30421 accgcaaggg actgggccac gccctgtccg atgaggatgc cgtccgggtc cttgtccgtg
    30481 aggggctgac cttccgtgat cttgagcaga ctgccgagct tggtttcctt gtccggctcg
    30541 acgccctcgc cgatgatggg caggtcgcgc cggccgttgt tgaggagacc cgagaaatag
    30601 acccggctca tcacggcttc cacctcggga tgcccgtcga agtggcgggc gatgtcttcg
    30661 ggatgatcga tcaggtagcg ctcgggatag cggatgccct tttcaaggaa ttccttcttg
    30721 aagacctgga tatggcccgt ctgggagtgg atgatggttt cacccagctg aatgaagatg
    30781 tcttccacga aaccgcccgc caggatcagc ccgacgaccc cgaagatgat cgcggcgagg
    30841 gtcatcccgg tacggaactt ctggcgcagg atgttgcgga tcgcaagtgt gaacggcatg
    30901 gtctcgagag caggtccggg caggcacgcg ggcgtgtggc catcggggcc ccgtgcccgg
    30961 atgacggcgc cggcttcgcc gcaatcccgc cggaatatta gcaggggcgc ggcgcaccgc
    31021 gaaggatggc ggcatgaact gtcgtatcat acctgttccg ccggcaaggc aaaccgggca
    31081 tttccatggt ccagttcgtt tacgacgagg ctttctcccg caatatcggc tgggtgacgg
    31141 tggccgaaca ggccgctctg cgaggcaagc gggtggcaat tgccggcatg ggcggcgtcg
    31201 gcggcgtgca tctgctcacc cttgcgcggc tcggcatcgg tgccttccac attgccgaca
    31261 tggatgtctt cgagctggtc aatttcaacc gccaggtggg ggccacggtg tccaacctgc
    31321 agcgccccaa ggccgcggcg ctggcggaga tggcccgcga catcaatccc gaactggaca
    31381 tccgtctctt cgagcggggc gtggatgacg ccaatctggg cgatttcctc gacggtgtcg
    31441 atctgtacgt ggacgggctc gacttcttcg ccttcgatgc ccgccgcgcc accttccgcg
    31501 cctgcgcggc gcgggggatt ccggcggtca ccgcggcccc cctcggcatg ggaacggcgg
    31561 tattgagctt cctgcccggc cagatgtcct tcgaggatta tttctgcctc gacggctgtg
    31621 acgaggagga gatggcgatc cgcttcctgc tcggcctctc gccggccatg ctccagcgcg
    31681 gctatctggc cgacccgtcg cgggtcaatc tggccgaaca gcgcgggcca tccacgattg
    31741 ccgcgtgcca gctgtgcgcc ggcgtcaccg cggttgaatc cctcaagatc ctgctcgggc
    31801 gcggtgacgt gctgtgcgcg ccacggggct tccagttcga cgcctatcgc aatcgctacg
    31861 tcaagacctg gcggccaggc ggcaaccgta atccattgca gcgcctcgcc ctgttcatcg
    31921 cgcgccgtca gctgcgcgcc atgcgccgct gaaccgtttc gagggaggtc atccaggtga
    31981 aacgtcccca cgctcctgtc gtttgcgcag gctcgcctgc ctgccaaccc ggtgccgatc
    32041 gggcggggtt gctgccatga tcccccgcga cacccgcctg cagatcctcg acctggcgcg
    32101 ctgggcgccc agtggcgaca acacccagcc ctggcgcttc gagatcgccg gtgacgaccg
    32161 ccttgtcgtc cacggtcacg atacgcgcac ctggtgcctg tatgactaca acggccacgc
    32221 cagccacatg gcccacggtg cgctcctgga gacgctgcgc atcgccgcca cggcccacgg
    32281 gctcgaggcc gtctggcgcc tgcgggcgga cagcaccgac accgcgccgg tctatgatgt
    32341 gaccctctcg ccggtctccg ggctggcagc cgacccgctg ctgtcctgca tcgagcggcg
    32401 caccgtgcag cgccgggcca tgcgtgcgac gccgcttgat tcgggccagc ggacggcgct
    32461 cgccgccgct ccgggcgcgg gctacaccgt gcagtgcttc gagacgggcg cggaacggcg
    32521 tgccgtggcg ggcttgctgt ggcgcaatgc gtatctgcgc ctgacctgcc cggaagccta
    32581 cccggtgcac aaggacgtca tcgagtggcg ggcccgcttc agcaaggacc gcattcctga
    32641 gcaggccgtg ggggtggatg ccgtcacggc acgcctgatg gaatgggtca tggccagctg
    32701 ggggcgggtg cagttcttca atcgtttcct cggcggtacg atcgcgccgc gggtgcagct
    32761 cgacctgatt cccgcgctgg cctgctcggc ccacctcctg ctgaaggccg acgtgccgcc
    32821 gcgcgacctc gcggaccacg tgcgggccgg cgcggccatg cagcgtctgt ggctcaccgc
    32881 cactgcggtc ggcctgcatc tccagcccga gatgacgccg gtcatcttcc ggtggtatgc
    32941 ccgcagcggg cagaggatct cccacgaccc cgccatcgat cgtggcgcgg cggccctggc
    33001 ggagcgcttc gagcagatcg cggccatcgg ccccgatcag gccctgagtt tcttctgccg
    33061 ggtgggggtg tccgatccgc cccgctcccg ttccctgcgc aagggcctgg acgaactgct
    33121 gctgaagcca tgagtcacgt tcacggcccg ggctgccggc acgggcatca ccacggacac
    33181 gcccatggcg cgcatgggca gggccccgcg catcacgacc gggcttttgc ggtgggcatc
    33241 gtgctgaacg tgctcttcgt ggccgtggag gcctggttcg gctggcgggc ggattccctg
    33301 gcgctgctgg cggacgcggg gcacaacctg tcggacgtgg cgggcctggc gctggcctgg
    33361 ggcgccgcct tcgcggcgcg cctgcgtccc acccatgtgc gcacctacgg ctggcaacgg
    33421 gcctccatcc tggcggccct ggccaatgcg attctgctcc tggtcgccat gggggcgctg
    33481 gcactggagg ccgtgcaacg cctcggcgcg ccccatccgg gcgacgccgt cacggtgatg
    33541 gcagtggcgg ccctcggggt cctgatcaac ggtctgagcg ccgctctgct catgaaaggc
    33601 agccaggacg atctgaacat ccgcggagcc ttcctgcaca tggcagcgga cgccctggtg
    33661 tcgctcggcg tcgtgggcgc gggggcgttg tacctggctt ttggctgggc ctggctggat
    33721 ccgctggtca gcctcgccat cgcggccctg atcgtggcgg gcacctgggg tctgctccgc
    33781 cagtccctgc atctcgcctt cgacggggtg ccggaggggg tcgatctggt cgaggtgcat
    33841 gcctggctgc gcagccagcc aggcgtgcgc agcgttcatg acctccacgt gtgggcgctg
    33901 gggacctcgg atgtggcgct cacggcgcat ctggtgatgc ccgccggtca cccgggggac
    33961 gccttcctca tcgccctggc cgacggactc gtgcaccgat tccggatcgg ccatcccacc
    34021 atccagatcg aaatcgacgg actgcccgac ggctgcgtcc ggcccgcgcg gctggagtag
    34081 gcctccccgt ccccgctcgg cacgaggggt cagatttcac gattccgcct gaatattcgg
    34141 gcgggccgtg gcgccattca ctgtccagac cccgaaaaag gcctatgttg caccgcaaaa
    34201 ggctgtgcta cagtgccaac accccaagag cgttgcagtg cgccactacc cataaaaaga
    34261 gtgcaggaga cacaggctgc ggccccttgc cgtggttgcc gccgtcacgc ccgtgacggg
    34321 gccgccacga tgcgggaccg gaatcagccc tcccatgcgg agggcagcaa gctggaggtc
    34381 ttcgcgccat ggctcctacc gccgttcgta ccgccgatgc ggaatccctg agtgccgccc
    34441 cccgggtggt cgccccgacc gatcgtggca gcgtccgcag ccgtgccagc cgctggcccg
    34501 cccgcctgga tgtgctgcag agcgtctccg gcctcttcct ggcggtcttc ctgatcgccc
    34561 acatgttctt cgtctcgtcc atcctgatca gcaaggacgc gttttatacc gtggcgcgtt
    34621 tcttcgaggg cgcctggctg cttggccggc cgcagccctg gctggtgtcc tgcgtcgtcg
    34681 gcttcgtcct gctggtcttc gtggggcacg cctggctggc attgcgcaag tttccggcca
    34741 atttccgtca gtaccgcgcc tttgccgccc atcgggcagg cctgcgccat gacgacacgg
    34801 ggctgtggtg gatccagctg tggaccggct tcgccctgtt ctttctgggg acggtgcacc
    34861 tctacggcat gctcacccag cccgagctga tcggtcccta cgaatccgcg gaccggatct
    34921 ggaccggcac cctgtggccc ctgtacctgt tgctcctgtt tgcggtggag atccatggca
    34981 gcatcggcct gtaccgcctc gcggtgaaat ggggctggct cgaagggcgc gatgcggcag
    35041 tcagccggcg ccgcttgcgc gtggccaaga cggtcttcag tgccttcttc ctgatccttg
    35101 ggctgatcac cctgcgcgcc tatgtgcaga tcggcgtgac ccacgccgac cgggccggtg
    35161 aacgctatgt gccgggtgcc cacgccaccc tggtgccgaa tccgtccgcc ctggcggcgg
    35221 cgccgaaccc gccgtccatg gagtaatcat gcgcatcgtc tataccgacg tgctggtgat
    35281 cggtggcgga ctggccggcc tgcgggccgc ggtcggcgcg cgccgacgcg ggcacgaagt
    35341 gatcctgctg agcctggtgc cgcccaagcg ctcccactcg gcggcggccc agggcggcat
    35401 gcaggccagc ctgggcaata ctgccatggg cgacggcgac aacgaggacg ttcacttcga
    35461 ggataccgtg cggggctcgg actggggctg cgaccaggaa gtcgcgcgcc tcttcgtcca
    35521 caccgcgccg caggccgtgc gcgagctggc cgcctggggc gtgccctgga accgggtcca
    35581 ggcggggccc catgaggtgg tgatggacgg ccggcgggtg acgctcacgg agcgtgactc
    35641 ggcccatggc ctcatcgccg cgcgggattt tggcggcacg cgcaagtggc gcacctgtta
    35701 tgtctccgac ggcaccggcc acgccatgct ctacgcggtg ggcgaccagg ccgtcgcggc
    35761 gggcatcccg gtccatgaac ggaccgaggc gctggccctg atccacgaag gcggccgctg
    35821 tcacggcgcc atcgtgcgcg atctcgtcac cggggccctg agcgcgtatg tggccaaggc
    35881 cacctgcatc gccagcggcg gcttcgggcg gatctaccag gtcagctcca acgcgatcat
    35941 caacgagggc atcggcgcag ccatcgccct tgagaccggt gtggcgaccc tggccaacat
    36001 ggaggcggtg cagtttcatc ccaccaccat cttcccggcg ggcatcctcg tcaccgaggg
    36061 ctgtcgtggc gatggcggtc tgctgcggga tgccgcgggt caccgcttca tgccggacta
    36121 cgagcccgag aagaaggagc tcgcttcccg cgacgtggtg tcacggcgca tggaggagca
    36181 catccgtgcc ggccacgggg tcaagacgcg ctatggcgac cacctgtggc tggacatcac
    36241 gctcctcggc gcggcccata tcgacggacg tctgcgggag gtgaaggaga tctgccagca
    36301 tttcctgggc atcgatccgt cccgcgactg gatcccggtc cgtcccgccc agcactattc
    36361 catggggggc atccgcacgg acgccagcgg gcacgtccgt ggcctgcgcg gcctgttcgc
    36421 gtgtggtgag gcggcctgct gggacctcca tgggttcaac cgtctgggcg gaaattccgt
    36481 cgccgagacg gtggtggccg gcatgatcgt cggcgagcgg atggccgatt tcgtggaatc
    36541 gccgatgggt gagctgcagg tctccaccgc gctggtccgc gactgtctcg agcggacaca
    36601 gcatcatctg gacacgctgc tgagcggcac tggcaccgaa gacgcccagg cgctgctcgc
    36661 ccgcatgcag gccatcatga ccgagcaggt cggcatcttc cggcggggcg acctgctcga
    36721 aagcgctgtc gccgaactgg cgtccctgct cgagcgcagc cgtcgtatcg gcatcgccac
    36781 gcgtcgcccg ggggccaacc cggaactcgt gaccgcctac cgggtgcaga agatgctcaa
    36841 gctggccctc tgcgtggccg atggagcgct gcaacgcacg gaaagccgtg gcgcccattt
    36901 ccgcgaggac ttcccccgcc gcgacgacgc cctgtggctc aagcgcaccc tggccacctg
    36961 gcccgacccg cagcagaccc ggccgaccct gagctatgag gccctcgacg tgatgcgcat
    37021 ggagctgccg cctggctggc gcggctacgg cgcccgcaat gccgtggagc atcccgacac
    37081 cgcgcgccgt gcggacgaga tcgcgacgat ccgcagcgcc ttcgcaacgg ccgaccgcta
    37141 caccgtacag caggccctga tgccgtacga acatctggtt ccgacgcgct tccgcggccg
    37201 aaaccaccgt tcaggagaac acacatgaac gccccgaccc caacccgcat cgtgaccctg
    37261 cgcctggcag accaccccga cgaatacgcc ctgctggaca ccgaaggact gaccctcctc
    37321 aacgccctca accacatccg cccgtccctg cccgcccccc tcggcgatgc cttcgtgtgc
    37381 catgcaggcg cctgtgccac ctgctacgtg ctggtgaatg gggagcgctg cctgccgtgc
    37441 accaccttcg tgcgggacct cccggacgaa ttcaccgtgg cgcccggcgt gcattcggcc
    37501 ggctgggacg agcagcgcca cgacgtgcgc cggggccacg gacaatgaag ccccgcgcgt
    37561 ccgcgggccc gcgcaccctg acgctgcgca tcctgcgctg cgggccggac atcgaccccc
    37621 actggcagga cttccgcatc gaggagacgg agggcatgac cctcttcatc gccctcacgg
    37681 aggtgcgcgc gcatcaggac ccatccctgc agttcgactt cgtgtgccgc gccggcatct
    37741 gcggcagctg cgccatgctc atcaacggcc gccccgggct ggcctgtcgc agcctgacgc
    37801 gggacctgga cccgctgatc accctcgccc cgctgccggt cttcgagctg atcggcgatc
    37861 tgtcggtgaa taccggcaag tggatgcgcg gcaccgccga acgcctcgaa acctggatcc
    37921 acacgacggc cgaggccgat ctcacggccg tcgaggcgcc catggagccg gccctcgccg
    37981 aagcgatcta tgagctcgac cgctgcatcg aatgcggctg ctgcgtggcc gcctgtggca
    38041 ccctgcgcat gcgcgaggac ttcgtcggcg cggtggggct caacaagatc gcccgcttcc
    38101 gcctcgatcc acgggacgaa cgcagcgacg acgatttcta cgagctgatc ggcgacgaca
    38161 ccggcgtgtt cggctgcatg tcgctgctcg gttgccacga tgtctgcccg aagggcctgt
    38221 cgctccagac ccagatcgcc ttcctgcggc gcaagatggc cgtgacgggg ctcaagtaag
    38281 tccgaccgtc gggacggggc ccgcgccggg cgatggcccg ggcctatccc ggtggttgga
    38341 agatctcatt ggcctgcccg ccgggccttc cggaaaatgg acaccacgcc atccatttca
    38401 ccgggagact gccatgacca ccctccgcat tgtccatcgg gacgcccgcg ccaacgctct
    38461 cgaccatccg gcccgctttt tcgccggttg cagcctggtc gtgctgggcg ccggcgtgtg
    38521 gtgcgcgcag gctgtgagcc acctgttctg acccgcctta tggcggttgc cccgccaggg
    38581 cggcgcggat cagggccgcc tcctcggcgc ggcgccgttt gaggcctgcc agcactggcg
    38641 ggcgcgcagc gagggcatcg atggcgtcgg cgagttcgcg ccagcggcca gcgtcgatga
    38701 agggcgcgag ggattcgacc cgtgcgccca gattgaccgc caccgacagc agggccgtat
    38761 ggactgcggg tggcgtgtcc gggcgcctga ggatgggatg cttgtcccgc aggcgcttcc
    38821 agaaaggctc gatcacgtcc ggcaccaggc gtgccacgtc ctccgcgcgg atgcggatgc
    38881 gtgccaggat cggggcggcg gcgtaggcgg cctgggcctc ggcgccgcgt ttgccggcgg
    38941 tggcgatgag ggcctggagc tccgcgggcg tcaggagaaa ttcgtagcgg cggcgcagat
    39001 cgtcgggctt tgccagcgcc aggtcggtga gggggtcgat gaagaccgat gagccgatca
    39061 gcgcgacccg ccccgcgttg ccctcccact tcatcaggaa ctcgaagccg ccgcgataac
    39121 cgatctgctc ggccagcctg gcgaggtact gacgggtgac atcggcgagg cgctggtcga
    39181 gcagcgcgcg ctcggcctgc atgggcgcga tctcggtcag gtggcctggt gtgatcgcgt
    39241 cggcgatgag cgtgtcgagg cgtgcgcgca gggcccgcag ttcggccgac tgggcgcgca
    39301 acagctcccg ttcgcttgcg gcgagggcgg cttcggcgcg ggctcgctcg gactcggcga
    39361 tgtggcgctg ggcttcggcg cgagcgcgct ctgcttcggc aatgtgccgc tgggcctcgg
    39421 cctgggcccg ttcggattcg gcgatgcggc gttgggcctc tg
//
LOCUS       DAOQPA010000018        38931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_419_length_38931_cov_18.121566, whole genome shotgun sequence.
ACCESSION   DAOQPA010000018 DAOQPA010000000
VERSION     DAOQPA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 38931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38931
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_419_length_38931_cov_18.121566"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..2551)
                     /locus_tag="PKX01_04295"
     CDS             complement(314..2551)
                     /locus_tag="PKX01_04295"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ75375.1"
                     /translation="MAAGKSTIIYTLTDEAPLLATCAFLPVVRTFTAPAGVDIVKSDI
                     SVSARVIAEFSDMLPDELKMPNTLAELGRITLQPDANIIKLPNISASVAQLKACIKEL
                     QEKGYPIPDFPENPTTDAEKAIKARYGKCLGSSVNPVLREGNSDRRAPAAVKNYARKN
                     PHSMGEWKQWSQTHVSHMEHGDFYHGEKSLTLDKARRVRMELIAVGGKTTVLKPEVKL
                     LEGEIIDSMFMSKTALCNFYEQQLEDCRESGILFSLHVKATMMKISHPIVFGHCVKIY
                     YKDAFEKHAKTFEELGINVNNGMVDLYEKIKALPESKRDEIIRDLHACQEHRPRLAMV
                     DSAKGITNFHSPNDVIVDASMPAMIRSGGKMWGADGKQYDCKAVMPESTFARIYQEMI
                     NFCKWHGNFDPKTMGTVPNVGLMAQQAEEYGSHDKTFEINEDGIANIVDIDSGEVLLT
                     QNVEAGDIWRMCQVKDAPIRDWVKLAVTRARNSNTPAVFWLDPYRPHENELIKKVKLY
                     LKDHDTTGLDIQIMSQVRAMRFTLERVIRGLDTISVTGNILRDYLTDLFPIMELGTSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVQQLTQENHLRWDSLGEFLALAVSLEELGIKT
                     DNAKAKLLAKTLDAATGKLLDNNKSPSPRTGELDNRGSQFYLSMYWAQELAAQTEDAE
                     LAATFAPLAKALTDSEDQIVAELKMVQGRPVDIGGYYRADSEKCKAVMRPSPTFNAAL
                     KAART"
     gene            complement(2846..3634)
                     /locus_tag="PKX01_04300"
     CDS             complement(2846..3634)
                     /locus_tag="PKX01_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75376.1"
                     /translation="MTARRLSACLALLALLGLTALPAAWAALPDEIQVYADDLNAPGE
                     FGLEVHVNTSPRGQRDATYAGEVKNDHSLFVTPELSWGLNDTMDAGLYLPTARDAATG
                     NLYAAGAKGRFKWLPIRGDELQGGGFAGLNLEIARIARRFEEYRSAAELRAMLGWRTP
                     DWLLAFNPVLSWDLSDGADRRTGYGFAFKGTHRIGEGLAGGFEYYTDQGKVGERQPWN
                     AQSNKLFAVLDVERHGWGFNVGVGKGLSTDTEKLTFKGILEFAF"
     gene            3766..4629
                     /locus_tag="PKX01_04305"
     CDS             3766..4629
                     /locus_tag="PKX01_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HMZ75377.1"
                     /translation="MRFRVRIAPIVLAWGAAMPAFAASDKLLLTGGVSQLEGAAGGGL
                     TPWAVIAGYGTRDQIGASAFYTQVRSQDYGLGVSGIAVGIRDRLELSYAHQSFDTRDV
                     GGALGLGNGFSFNQEIFGAKLRLLGDAVLEQDSWLPQIAIGLQHKRNDQGAVLHAIGA
                     KKDHGTDFYVAATKLFLDQSLLANLTLRETKANQFGILGFGGDLKDRYTTQFEGSLAY
                     LLRRDLAVGVEYRTKPDNLGIAREDKAYDAFLAWQPVKNVAVTLAYANLGNIVIKDNQ
                     RATYLSVQLGF"
     gene            4664..5089
                     /locus_tag="PKX01_04310"
     CDS             4664..5089
                     /locus_tag="PKX01_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HMZ75378.1"
                     /translation="MKTFSFCAALALALMPVASHAEADLSTYRAFGEKPGLVSLMDAF
                     MVNLLADGRTRPFFEHADQTRIKAQLVDQICAVLDGPCTYQGAPMGPLHAGLGIQQGH
                     FNALVEDLQQAMDAQGVPFRAQNKLLARLAPLHRDIVTR"
     gene            complement(5104..5904)
                     /locus_tag="PKX01_04315"
     CDS             complement(5104..5904)
                     /locus_tag="PKX01_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HMZ75379.1"
                     /translation="MPAAPTATEHDDAAPYPSITVPEPLLCRPSKGREAPIPLVDGEV
                     HLWQVQLPIRDTALCRAVESATTANERARAGRLPFEDDRLRQLYTRGMLRILLGHYLS
                     KSPNALNFPLNPWDKPLLEEDPGLHFNVSHAHSLALIGVTRSAPVGVDLEAVRPLDDR
                     EEQFRRTLCSDELSWIDRFSEPLRRNHEFMRLRTGKEAALKALGMGLARHLDSFGVVP
                     PPSPVGPGEVNGLRGRWTLHFPPLPQGFAGAVAIATDSAGPLRVFRVG"
     gene            complement(6095..7060)
                     /locus_tag="PKX01_04320"
     CDS             complement(6095..7060)
                     /locus_tag="PKX01_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HMZ75380.1"
                     /translation="MKLVHRLLRRSALVLARTVGGRPFATRIRHLAAQHYPPSAAGRA
                     GEATRFHLRSLIFAGPTRSWFELLESDARLQHLVRLNPHLAEKIHRPYLRCHYSPSRR
                     LAALSTHYRFCLDHPQGGLLLHAAAGRTVPVGEILGKQDSVFRAVLTSEEQFMKEGEF
                     VIQLHDEHGRLYSIAAAIVGSRGGPVLSVGCMQGPGQGENGSARIKAATKAMWGLRPK
                     AAILLIAQNLAENFGLTGLVAVGNDHHIYRSWRKRRTIHTDYDALWADLGGTPRPDKD
                     FDFPVVPPVRPISDFPSGKRADAQRRQTLREALAVSIRSHLAAAT"
     gene            complement(7074..8438)
                     /locus_tag="PKX01_04325"
     CDS             complement(7074..8438)
                     /locus_tag="PKX01_04325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ75381.1"
                     /translation="MSPSLRKSLLLWLLLPAGLAVLTFLPLAYHLVHQPAEEALDRAL
                     ADASLALVPHLEVRNGEPHFVFPPAAEQVLRADRVDQIYYLVLGPGERFVAGDAGLPH
                     QPEGEEIHGERVSFNSHFRGQPIRVTAVHHSVAGEPFIFVTAETLRKRNQLAVDLAFA
                     LAMPLFFFAAATGFTIWFGIRHALLPVEDIRRSLHGMEHHALQPLDALSAPAEIRPLV
                     VEFNAVLDRLERASDAQQRFVANAAHQLRTPLAGIRTQLELLLRDADPEQRRNRTTQC
                     IAALERLGHLIGQMLVLISAEPGGRDAAAGSLLDVPEIIRERSPDWIRLAEAKGIDLG
                     FELGPVRIRGDGLLLGELIANLVINAITYTPAPGEVTVRCRQSSDHAEVDVEDSGPGI
                     PAEARELVFERFYRLPTSTAPGSGLGLAIVREIAGRFGGRALIAAPPGGKGTLVRVSL
                     PASA"
     gene            complement(8419..9111)
                     /locus_tag="PKX01_04330"
     CDS             complement(8419..9111)
                     /locus_tag="PKX01_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75382.1"
                     /translation="MNARVLIVEDDPLLADGVAQVLRDAGFVTDYVGTAEQAEASLAA
                     EAFDLLVLDIGLPGMDGLELLRRLRARSSAIHVLMLTARDALNERVRGLNAGADDYLT
                     KPFAAVELLARVNALARRSRGQRGARREFGPLILDEEAYRAWLDGEPLTLPGREWAVL
                     LFLLDNQGRVMSKERIAAAVCRWDQDMSENAVEVYISRLRAKLETAGIRIRTVRGLGY
                     MLENLRDEPKPA"
     gene            complement(9152..12244)
                     /locus_tag="PKX01_04335"
     CDS             complement(9152..12244)
                     /locus_tag="PKX01_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ75383.1"
                     /translation="MFDALITAALRQRAFFLIAALALLGYGIYAFRQISIEAFPDVQD
                     VQVQVVTQVPGQAPEEVERTVTLPIEREMSGVPGMTQLRSVSITGLSVVTLTFSENTG
                     DYFARQQVLERLQTVNLPPAIQPTLAPLTTAVGEIYRYVLESPPDTPLEEIRAVQDWS
                     IRPALRIVPGIADVVSFGGAVREIQVRIDPDLLRKYNVSLNDVSQALATASANGGGGL
                     LRRGDEALVVRALGLFNTLDDVRDVVIVARDGKPVRISDVAEVASGARPRAGIVAFND
                     RDDVIEGIVQMTKGGNAAKIVEALRAKVDEVNARLPEGYRIRPIYQRTELIGHTVATV
                     GENLLVGAFLVTGILLVFLRNLRGAVIVAAVIPLSLMFAFILMHAMGVSANLISLGAV
                     DFGVIIDSAVVLVEALMVRLAVDAKAEHHISSPVGRRLYALKHTCVELGQPILFSKAI
                     IIVAFLPIFTFQRVEGRIFTPVALTLSFALLGGLILTLTLVPTLLSFALNKDMAEKES
                     HWLHRLQENYRSLLLGLQARRRSMVVGSIALLITSLGLAPMLGSEFLPKLDEGNIWLT
                     ISLPQSSALGNTKDVERKVRAIIASYPEVKAVITHVGRPDDGTDPKGPNNMEILVDLK
                     PRGEWRFPYKDDLVDSMAARLSTIPGLPTNFSQVIQDNVEESLSGAKGEIAVKVFGPN
                     LEVLEAKGRQIADILNRIQGSADVAALPVGGQSEVDIRLHREALARYDINVTDVNALI
                     QTALGGSAVNAYYEGDRRYDVTLRFGAAFRDQVDDIATLPVPLADGVGSIPLGDLADV
                     ELRQGASRIQREAGSRNVIVKANLRGRDQGSFVAEAQAKVAAEVSLPKGYTITWGGQF
                     ENQQRAMGRLKVIVPITLGLIFSLLFWAFRDVRPAFLVLSMVPFTLIGGFAGLGLAGL
                     HLSVSAAVGFIAVAGISVQNGLIMVEQVIEISRRGASRAEAILEGAVLRLRPIIMTAL
                     MAGLGLLPAALSHGIGSETQRPFAVVIVGGIVSATLFTLLLLPVLLSFGGAQSLRDKE
                     "
     gene            complement(12250..13410)
                     /locus_tag="PKX01_04340"
     CDS             complement(12250..13410)
                     /locus_tag="PKX01_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75384.1"
                     /translation="MNRKQIAIAAVVAIAVAAAVYLRITRVGEDTPDTPVVQKPSAEP
                     GLLRFPTAAPQLAQLKVDAAAVSPVPMDEPLAAKVAYDENATARLFAPVAGRVIEIHG
                     QIGDTVKAGAVLAVLDAPELGAAEADVSKADAEASRTKAAFERARSLFEAEVIARRDL
                     ESAESDWRQARAEADRARLRLTNLSPGGGAITGQRYTVRAPIGGVIAARHINPAMEVR
                     PDAQDPLFVVTDLSRLSVLVDVPERDLPLVTVGKAAVVEASAYPNRNFDGQVVHVAPT
                     LDPATRRVQARVSVDNREKLLKPEMYARVSLLADAREELPRIPTAALLVEGVKNFVFV
                     ERAPGEFEKREVTLAVQTRQFAYVWHGLKGGEKVVSAGALLLNAELASSSAR"
     gene            complement(13407..14645)
                     /locus_tag="PKX01_04345"
     CDS             complement(13407..14645)
                     /locus_tag="PKX01_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ75385.1"
                     /translation="MPRLFTPAFAAALMVPALCLAQASVSPLTLADADRLLLDANPAI
                     LQARHQLTGAHAGIDIAGARPNPQLTLGVSTIDTGGSRWRRPTDNSVRIDQTFERGDK
                     RDLRLKAAERGVQAADADLTNTIRLARIDLANAWIDLRTAQDVQRASDESAAFATRTA
                     QAAELRGKAGDLAGVDVARYAADAARVANDAVQAGLQLAQARIALSQILGNRIAPNQL
                     RAADTWPDEATNSVAANAERADIAAARRRLEQADALRGLARAQRSRDLSVGVQYEHTP
                     ASLNNAVSENTFGLSVSVPLFLGYDYRGDIARSEADYLMAEQELARIRAAVDGERLRL
                     KAEVAAADARYRRLQDIALPAAERAARGAEVAIQKGGMSLTDYLDTQRGLRAARIETI
                     QARADLARARQALALAGDTP"
     gene            complement(14768..15244)
                     /locus_tag="PKX01_04350"
     CDS             complement(14768..15244)
                     /locus_tag="PKX01_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75386.1"
                     /translation="MSGRIIFSGPSGAGKTSAIDVASDIPAIWKGNADYGAMTLDDGG
                     VIHLCSSACETATGVSGDDSLGLILLIDNRRTAPDDDLLRHLRSFDAFIRRTAVAVGI
                     THMDQQPRPRIDLFHTVMSYAGHGGMPLFEVDARDRRDICLLVEAMVSVLDPVACA"
     gene            complement(15241..16404)
                     /locus_tag="PKX01_04355"
     CDS             complement(15241..16404)
                     /locus_tag="PKX01_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75387.1"
                     /translation="MTSARSALRLCHAGMEGRTLDAFQIFLSRVDAFSCRITDENHAD
                     AAFIDADGDLGQYLLEGHRLLYPGRPMIVTSSRPTPNHDPFTIQVTKPVGLAAFSAAL
                     QRVHELLPRSRDDGRRAPQPPFYGDLGAPPDDLPAFTDAVEAETIFATRYVGSAPDVD
                     LDDPRQRERIYYLPENFLQGTLARALAHARDVERPVKLRGPLGTILYLDPTNESAYQP
                     LSPKALRAQAQVPTRGTIKQTTLDAGDVGPVARLDPRPLQVVEWEVALWASRGRLPRG
                     TDLDTPVRLVRWPNFTRLPVPEGALRIAGLWSRTPTTLRDTVHLLGLPQRHVFAFFSA
                     CAALDLLQLGDEGVPDRRGTGDPARTPERRGLLQRTLGRLLGARPDERPDPRP"
     gene            complement(16658..19786)
                     /locus_tag="PKX01_04360"
     CDS             complement(16658..19786)
                     /locus_tag="PKX01_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ75388.1"
                     /translation="MLAALIDWSGRNRFLVLLATLCVVLAGVYAVLRTPIDALPDLSD
                     VQVIVYTEFPGQAPQVVEDQITYPLTTAMLSVPKSRVVRGFSFFGVSFVYVIFEDGTD
                     LYWARSRVLEYLSAAAGRLPAGIMPQLGPDASGVGWVYQYAVLARDKTLAELRSLQDW
                     TLRYPLAKARGVAEVASVGGFVQTYQVTVDPVKLRAYGIPLSRVAQVIRASNRDVGGR
                     VVEMAETEYMVRGRGYLRGVDDIASLVVKAERGTPVLVRDIGRVELVPDERRGLAELD
                     GEGEAVGGITVARSGQNALDVIRHVKATLADLAPGLPDGVTLKTVYDRSELIQRAIAT
                     LRHTLLEESLIVAAVCVVFLLHVRSALVAILMLPVGVLIAFVALHLLGMNSNIMSLGG
                     IAIAIGAMIDAAIVMIENAHKHLERLPDDAPPAARAAALLAACREVGPALFFSLLIVT
                     VSFLPVFTLEGQEGRLFAPLAWTKTFAMAGAALLSVTLVPVLMGLFIRGRVLPEARNP
                     VNRLLIRVYRPVIAAVLAWPRLTVVLAGAVLALTAIPATRLGAEFMPPLDEGTLLYMP
                     TSLPGLSVTKAAELLQIQDRIIKSFPEVDSVFGKAGRAGTATDPAPVEMFETVITLKP
                     HEQWRPGLTTDGLVAEMDKALQFPGVANAWTMPIKARTDMLATGIRTPIGIKVFGRDL
                     DEMERLAKDIETVVRQVPGTTSAFAERLTGGLYLDIEPDRARLARYGLSVGELQEVIA
                     GALGGEAVTTTVEGRERYAVAVRYPRELRSDPERIAREVLVPVDGGAMVPLGQVATVS
                     IARGAPGIRTENAQLAAYLYVDLRDRDIGGYVADAQRAVAEQVHFPPGAYVTWSGQFE
                     YMERARERLQVVVPITLLSIFMLLYLNFRRLTETFIVMLSVPFALVGGVWLLAALGYN
                     LSVAVGVGFIALAGVAAETGVVMLIYLDHAWADALARCRAEGREASRADLLAAVTSGA
                     VERVRPKMMTVVAIMAGLLPILWSRGTGSEVMSRIAAPMVGGMLSSTVLTLAVIPALY
                     LLVKGRALAGRP"
     gene            complement(19797..21338)
                     /locus_tag="PKX01_04365"
     CDS             complement(19797..21338)
                     /locus_tag="PKX01_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75389.1"
                     /translation="MSAPRPALRLAAALLALAAAAGAGYGLGQRHGTEPPAASPTPAA
                     PAARKLLYYRNPMGLPDTSPVPKKDPMGMDYLPVYEGEADAEAAPGQVRLSPEKIQKL
                     GVRTEAVTLRALDRSVDASGRVEPDERRVVAIAPKFEGFVERLHVSVTGQPVARGQAL
                     FEVYSPELVSAQREYALAAQGADSLKDAGPEAQKGLRQLADAALQRLRNWDIPDAQIR
                     ALTHSGEARRTLTFTSPAAGIVTEKKAVQGMRFMPGETLYQVTDLSAVWVVADVNEQD
                     IGAVRPGSPVKLRVDAYPGETFDGRVSYIYPTLNADTRSVPVRIELANPSLRLKPAMF
                     VHVSLPAGSAAPVLTVPSAAVLDSGTRRVVLVERGEGRFEPREVRLGARSARYVEVLD
                     GVKADERVVVAANFLIDAESNLQAALGAFTATGGTPPAAQAASVVHRAEGQVDGADAA
                     AGTVTLSHGPVASLNWPAMTMDFAVANPALQAALKPGARVAVDFVERKPGEWVITAVR
                     PAAGR"
     gene            complement(21335..22606)
                     /locus_tag="PKX01_04370"
     CDS             complement(21335..22606)
                     /locus_tag="PKX01_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ75390.1"
                     /translation="MKPPRLVHLLLIGCAVALPAAACAQEAPLGRDVEGLLAWARAHS
                     PELAAMRHEADAAAERVVPAGALPDPRFRTELRDITRMGEQSPALLPARTGSTRYLLM
                     QDLPWAGKRDLKQAAAEQDARSASGRADATWADLSARIKITWTQLHYVHQSTRLTREL
                     LDLGGRIGQIAQVRYAGGLAPQQDAIRAQVELTGLQADLVALDNEGRQLQVRLNALLA
                     RPSAAPLAAAERLRPLPVPARLDPAALEDRLRARNPQLFADEARVAAAERNQALTLRN
                     RYPDFTVGISPIQYQNAVREWELMVELNLPLQQASRRAQEREADSLLAAARARREATA
                     QQALGELGEALSALDAARRTEALASQSLLPQAELTFRAALAGYETGKVDFTTLLDAQR
                     QIRLARQNRLKAQTEAQLRLADIERLVGEDL"
     gene            complement(22620..22943)
                     /locus_tag="PKX01_04375"
     CDS             complement(22620..22943)
                     /locus_tag="PKX01_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HMZ75391.1"
                     /translation="MRLRLLPAAPLAAVLLAALPAIAAEPTAHAAHATAAQALTDGVV
                     KKIDKAAGRVTLTHGPLNGMPGMTMAFAVKDPAWLDTLKPGERIRFAADTVNGALTVV
                     KIERP"
     gene            23107..23790
                     /locus_tag="PKX01_04380"
     CDS             23107..23790
                     /locus_tag="PKX01_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HMZ75392.1"
                     /translation="MKILIVEDESKTGDYLRQGLVEAGFVADWVQDGLDGLHRGLTEA
                     YDLVILDVMLPGADGWQVLQGLRRAGKDLPVLFLTARDQVDDRVKGLELGADDYLVKP
                     FAFSELLARVRTLLRRGARAREPDVLKVADLELDRPRRRVTRAGRRIDLTAKEFALLE
                     LLLRRRGEVLPRSLIASQVWDMNFDSDTNVIEVAVRRLRAKVDEGFTPRLIRTVRGMG
                     YVLEETPWD"
     gene            23781..25169
                     /locus_tag="PKX01_04385"
     CDS             23781..25169
                     /locus_tag="PKX01_04385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HMZ75393.1"
                     /translation="MGLNRRLSLTVRLTLLFALASSAVLLILGFIVGASVERHFEELD
                     RATLYGQLADLRRGLALGHLHGEAGGEGGLVEGAHLHPPMMELRGPDGRVLAASPGAV
                     FPSDVVIGAPGPQVWQDASGHWFRGLAAELPAAPAGAGPLRARVATDISHHQAFMDGF
                     RRTLWGVVVASALATGLLGWAAARSGLAPLEAMRRTAAQVSAQRLDPRLAVDSVPVEL
                     AALAESLNAMLARLEESFRRLSDFSSDLAHELRTPVSNLMTQTQVALSRPRSAAEYRE
                     VLESGAEELERLTRTIADMLFLAKADNGLIVPAREPVELADEVRALFEFYAALAEEVG
                     VSLSQAGAGRVAGDRLMLRRALGNLLSNALRHTPAGGAVRVTLGNAEDRVVVAVANDG
                     EPIPAEHLPRLFDRFYRADPARRHGSDGDGAGLGLAITRSIVRAHGGEVVVRSGVHGT
                     VFQMRLPPAGGS"
     gene            complement(25241..26683)
                     /locus_tag="PKX01_04390"
     CDS             complement(25241..26683)
                     /locus_tag="PKX01_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ75394.1"
                     /translation="MTPRAALVSGAIALLLSACSAVGPDYRLPDAAQLRSPDARQAFQ
                     EAGQPPFGRDDLPYAWWRLYEDPALNDLIARALVANAGLRAAAANLARSEAVVREVDG
                     LRDPSLGAGVAIERAQVSAESYLLEHKVPVFNLGNAGLRAHYQVDLAGQLRRALEVAE
                     AEAGAVQAAGDLARISVVADTALAYMDACAAGEALDLARRALALQVREREAIARLVAG
                     GRRIPLDLPRAAEQEDQLRAALPAFAARRRSAAYRLAALTGQSPARAPAAAEACRHLP
                     DLAQPIPVGDGAALLRRRPDVRQAERRLAAASARIGVATAALYPSLSFGLSAGGTGIL
                     SHLGEAPTQRWSLGPVLSWSLPGEEARARVAQADAGAAGALATLDQVVLNALQETESA
                     LATLARDRDRHAALQQARAHAETARRQVHTLYQAGRTGVLDDLDAERRLLAADARLAA
                     HRGRLATDEIRLFLALGGGWQPGEVHTTPQ"
     gene            complement(26680..27576)
                     /locus_tag="PKX01_04395"
     CDS             complement(26680..27576)
                     /locus_tag="PKX01_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HMZ75395.1"
                     /translation="MNTTFLSKAAPVLLTLAIVAVALIAGRQLWIHYRSEPWTPDGRV
                     RADIVQIAPDVSGTVTDLAVGHDQPVKAGQFLFQLDPERYALALHDAESAERVQEARL
                     AQLQREVRRNQTLGELVSREAAEQTQAHLDEARAALAQARAQRELAALNLKRTRIVAP
                     ADGTLSDLTLRVGNYVAAGKPVMALIDSRSLRVEGYFEETKLPRISVGQTARVHLMGD
                     DRILTGHVQSIAAGIEDRDRTSGSSLLPNVNPTFSWVRLAQRIPVRIALDAPHDPALI
                     AGRTATVTLLPPTAERDGGPAR"
     gene            complement(27600..27809)
                     /locus_tag="PKX01_04400"
     CDS             complement(27600..27809)
                     /locus_tag="PKX01_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HMZ75396.1"
                     /translation="MNGEIELFGVYLSAELSTSIAALLLTFGLQRGLVWLGLLRRVWH
                     RPLFETALFIIVWRLVVAAWALHSP"
     gene            complement(27806..29854)
                     /locus_tag="PKX01_04405"
     CDS             complement(27806..29854)
                     /locus_tag="PKX01_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HMZ75397.1"
                     /translation="MIRPEPLLRLDWRDVQFSVVSLAAALAALYWSMSLDLQQPYWSM
                     LSAYVVSQPLAAAVRSKAVYRLAGTLLGAAMTVLLLPALANTPELLCAAMALWVGACL
                     SISLLDRSPRSYVLMLAGYTAAIVGFSSVNQPGQIFDLAVARAEEISIGILCATLAHS
                     LWFPRSVGEVLEQRIRDWLGEADRWALDLFAADDPSAGVPHRAHLAAAATDIHLLAAH
                     LPFDTSHLRETTAIVRALHDHILLLIPVLSSLSDRLAALHGMRAPVDPPTQDRLTRVG
                     AWIRADCPAAEGRALSAMLAADLDTPAAAPDAASWYALNRNGLTTRLLDLVQIQTECR
                     QLLAHLHEPDAPLAPALRDALGQAGARPLHSDWRLALTSGAVATTAILICCAAWIGLG
                     WSEGGASAMLAAILCCLFASLDDPAPAIRKFGVAICIAVSLAALYQFAVFPAINTFPL
                     LALVLAPPLLAIGAATLNPRLASSALFILLNFCNFMAIQERFTPDLAVFLNNNLSQFF
                     GVFVAIYVTRAARSLSTASSARRLARATWQDLARFAHGDVREPHQNLTARLVDRIGLL
                     APRLAALRDQDAELADGDVLRELRIGMDLSTLQDAHPPLPGIAQTQVTELLHAVGRHY
                     QQRPDGTARPPQDLCALLDGCIRHLGGPPGQGRALAALVGLRRNLFPDTPYAAGGLNP
                     "
     gene            complement(29841..30308)
                     /locus_tag="PKX01_04410"
     CDS             complement(29841..30308)
                     /locus_tag="PKX01_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ75398.1"
                     /translation="MTTPPSALRRQLTTLLIRAARLYRKEVNRALTTYGISDAQAIPV
                     LHIARHGGGMRQHELAEEIGIEGPSLVRLLDQLCAHGLVERRSDPQDRRARTLHLTDA
                     GEALAARVETALVGIRGRLLAAVSEADLSTCLRVLETLLHQLDVGPSHHDDPT"
     gene            30821..31111
                     /gene="groES"
                     /locus_tag="PKX01_04415"
     CDS             30821..31111
                     /gene="groES"
                     /locus_tag="PKX01_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMZ75399.1"
                     /translation="MKIRPLHDRVIVKRIEAERTTSSGIVIPDSAGEKPDQGEVLAVG
                     NGKVLDNGEFRKLEVKVGDRVLFGKYAGQTVKVDGQELLVMREEDIMGVVEA"
     gene            31163..32812
                     /gene="groL"
                     /locus_tag="PKX01_04420"
     CDS             31163..32812
                     /gene="groL"
                     /locus_tag="PKX01_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMZ75400.1"
                     /translation="MAAKEVKFGDSARARMVEGVNILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFANMGAQMVKEVASKTSDIAGDGTTTATVLAQSIVRE
                     GMKFVAAGMNPMDLKRGIDKAVAATLEELKKLSKPCSTTKEIAQVGSISANSDASIGD
                     RIAEAMEKVGKEGVITVEDGKSLADELDVVEGMQFDRGYLSPYFINNPDKQVAILDAP
                     FVLLFDKKISNIRDLLPVLEQVAKAGRPLLIIAEDVDGEALATLVVNNIRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEEVGLNLEKATLQDLGQAKRIEVGKENTTIID
                     GAGNAERIEARVKQIRVQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRARASLAGLKGDNHDQDAGIKIVLRAMEQP
                     LREIVGNAGDEPSVVVNKVVEGSGNFGYNAASGEYGDMVEMGVLDPTKVTRTALQNAA
                     SVAGLMLTTDCMVAELAEDKPAAGMPGMGGMGGMGGMDMGM"
     gene            33018..33326
                     /locus_tag="PKX01_04425"
     CDS             33018..33326
                     /locus_tag="PKX01_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HMZ75401.1"
                     /translation="MKDDLGDRIRANPKYQELVSKRNSYGWIMTILMLAVYYGYILLV
                     AFNKEFLAQKLGAGVMSLGIPIGVGVILFTILITGIYIRRANTEFDDLKAQVIKEATK
                     "
     gene            33326..35095
                     /locus_tag="PKX01_04430"
     CDS             33326..35095
                     /locus_tag="PKX01_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HMZ75402.1"
                     /translation="MRTSKLILGLLAVLGSGAALAAGADLGQAEKQATNWTAIIMFGV
                     FVAFTMFITKWAAGKTKSAADFYTAGGGISGFQNGLAIAGDYMSAASFLGISAAVMAN
                     GYDGLIYAIGFLVGWPIITFLMAERLRNLGKFTFADVAGYRFQQTPIRAFAASGTLVV
                     VAFYLIAQMVGAGQLIKLLFGLEYWVAVVVVGALMMVYVLFGGMTATTWVQIIKACLL
                     LAGASFMAFMVMSKFGFSPEAMFAKSVEIKTALAAKDAKLLEEAAKVAAEKGKEVTEV
                     VAAMAAKKGDSIMGPGSFVKDPISAISFGMALMFGTAGLPHILMRFFTVPDAKEARKS
                     VGWATVWIGYFYILTFIIGFGAICFVLTDPSFLDAKGALKGGGNMAAIHLANAVGGNV
                     FLGFISAVAFATILAVVAGLTLSGASAVSHDLYATVFKKGNADSAAELRVSRITTIVL
                     GIVAVILGIAFEKQNIAFMVSLAFAIAASANFPVLFMSVLWKDCTTKGAVIGGFLGLI
                     SAVGMTVLSDSVWVAVLGNPKGSAPFPYTSPALFSMSIGFVGIWIFSLMDRSPAAQQE
                     REDFAAQLIRSETGIGAASASSH"
     gene            35530..36204
                     /locus_tag="PKX01_04435"
     CDS             35530..36204
                     /locus_tag="PKX01_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75403.1"
                     /translation="MRILLAEDDLIIADGLCRALKKAGYAVDHVPNGADADAALLGQT
                     FDLLILDLGLPKLSGIEVLRRLRARKCTLPVLILTAQDGIEDRVRGLDAGADDYLTKP
                     FALPELEARVRALTRRGTGQARCIEYARLAYDQADRVVKIDGQVVDLSAREIGLLEVL
                     LLRAGRLVSKEQLVDHLCGWGEEVSNNAIEVYVHRLRKKLEQSGVQIVTVRGLGYCLE
                     KPDVVA"
     gene            36191..37702
                     /locus_tag="PKX01_04440"
     CDS             36191..37702
                     /locus_tag="PKX01_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ75404.1"
                     /translation="MWSPKWRRVADDGAADGKGFNSLFGEILDWMLAPLLFLWPISII
                     VTHNVANSIANQPYDQALAVNVRAIARLVRVEGSQARVNFPAPARLILRADEDDTVYY
                     QVLGFKGELVAGDKDIPTLQTPDSVVPDVVLFRDESIAGEDVRVAYQYLQPDAAAPRR
                     LVLLQVAETRNKRSTLASRVVTGVLLPQFAIIPIAVILVWLGLSRGIAPLNQLQRLIR
                     RRRPTDLSPISVSNVPEEVRPLIIAFNEMMARLEENLQAQQRFIADAAHQMRTPLTGL
                     RMQSDLALMETDPAQIHRSLQHISQSAERASHLINQLLSLARAEASYEKIYAVERVDL
                     EALLRAMLTDFVPRARQKAIDFGLETTGGPLVIDGNPVLLAELLKNLIDNALKYTPEG
                     GRVTVRTLATEKAIVEVEDTGIGVPEADRERVFERFYRVLGSSESGSGLGLPIVREIA
                     ELHRAHVSLNPNPAGRGTIAQVVFPRSRPTPEETAAQTTQALTAPGATQFPPA"
     gene            37771..37846
                     /locus_tag="PKX01_04445"
     tRNA            37771..37846
                     /locus_tag="PKX01_04445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37804..37806,aa:Phe,seq:gaa)
     gene            complement(38110..38793)
                     /locus_tag="PKX01_04450"
     CDS             complement(38110..38793)
                     /locus_tag="PKX01_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75405.1"
                     /translation="MALDEDWLRQKMRLALDIESRDDIDDLGDGVWRLGNARSSPVVL
                     ARDLTRVLHEPSLLDRVRVAGSNIRVITPRPRTTRGSPFGSGVEWLALEERFTFYGCG
                     ITFIPSVPSAVPAVADPAAPVNGPFSADFKWATLPDVQATPIRFTDGQAKVFEALWSF
                     KGAEVNGERIMQRAGQKSDKPIDLFKIKSKDKGKPEHEARLAAYGALVVTQQRAGLYS
                     MPCAAAVLA"
BASE COUNT         5946 a        12475 c        14329 g         6181 t
ORIGIN      
        1 tgtggatgtg cgggaggatc tgggcggctg agggggcctc gtgccccgtg gggggtggcg
       61 ctgcccggca cgcatcgatc ctgtcggcgt gtgacgcgga tcccgggcct gaatgcgatg
      121 cacggtttcg cgaccgtcct gcccaaacgg agcgtcgagc ttctgcgcag agaccggcac
      181 ggcgcgcggc gcccgatgag gcaaggcgcc agggcttggt caggaaattc cccagacgaa
      241 aacgccggca gacccgtgtc caggtctgcc ggcgttgtct tgtcagcgct cgatgcgctg
      301 agggcgcgtc acatcaggtg cgggcggcct tcagggcggc gttgaaggtg gggctgggac
      361 gcatcacggc cttgcacttc tcggagtcgg cgcggtagta gccgccgatg tccaccggac
      421 ggccctggac catcttcagc tcggccacga tctggtcttc gctgtcggtg agggccttgg
      481 cgagcggtgc gaaggtggcg gccagttcgg cgtcctcggt ctgggcggcg agttcctggg
      541 cccagtacat ggacaggtag aactggctgc cgcggttgtc cagctcgccg gtgcggggcg
      601 acggggactt gttgttgtcc agcagcttgc cggtggccgc gtcgagggtc ttggcgagga
      661 gcttggcctt ggcgttgtcc gtcttgatgc ccagctcttc cagggacacg gcgagggcca
      721 ggaattcgcc gagggaatcc cagcgcaggt ggttttcctg ggtgagctgc tgcacgtgct
      781 tgggcgcgga gccgccggcg ccggtttcgt acatgccacc accggccatc agcgggacga
      841 tggacagcat cttggcggag gtgcccagtt ccatgatggg gaacaggtcg gtcaggtagt
      901 cgcgcaggat gttgccggtg acggagatgg tgtcgaggcc gcggatgacg cgctcgagcg
      961 tgaagcgcat ggcgcgcacc tgcgacatga tctggatgtc gagcccggtg gtgtcgtgat
     1021 ccttgaggta gagcttcacc ttcttgatca gctcgttctc gtgcggacgg tacgggtcga
     1081 gccagaacac ggccggggtg ttggagttgc gggcgcgggt gacggccagc ttgacccagt
     1141 cgcggatcgg ggcgtccttg acctggcaca tgcgccagat gtcgccggct tcgacgttct
     1201 gggtgagcag cacttcaccg gagtcgatgt cgacgatgtt ggcgatgccg tcctcgttga
     1261 tctcgaaggt cttgtcgtgg gagccgtact cttcggcctg ctgggccatc agaccgacgt
     1321 tgggcacggt gcccatggtc ttgggatcga agttgccgtg ccacttgcag aagttgatca
     1381 tctcctggta gatgcgggcg aaggtggatt ccggcatcac ggccttgcag tcgtactgct
     1441 tgccgtccgc gccccacatc ttgccgccgg aacggatcat ggcgggcatg gaggcatcga
     1501 cgatcacgtc gttgggggag tggaagttgg tgatgccctt ggcagaatcc accatcgcca
     1561 ggcgggggcg gtgttcctgg caggcgtgca ggtcgcggat gatctcgtca cgcttggatt
     1621 cgggcagggc cttgatcttc tcgtagaggt cgaccatgcc gttgttgacg ttgatgccca
     1681 gctcttcgaa ggtcttggcg tgcttctcga aggcgtcctt gtagtagatc ttgacgcagt
     1741 ggccgaagac gatggggtgg gagatcttca tcatcgtggc cttcacgtgc agcgagaaga
     1801 ggatgccgga ttcgcggcag tcctcgagct gctgctcgta gaagttgcac agggccgtct
     1861 tgctcatgaa catggagtcg atgatctcgc cttccaggag cttgacttcg ggcttgagga
     1921 cggtggtctt gccgccgacg gcgatgagct ccatgcgcac gcggcgggcc ttgtcgaggg
     1981 tcagggactt ttcgccgtgg tagaagtcgc cgtgctccat gtgggacacg tgggtctggg
     2041 accactgctt ccattcaccc atggagtggg ggttcttgcg ggcgtagttc ttgacggcgg
     2101 cgggagcgcg gcggtcggag ttgccttcgc gcaggaccgg gttcaccgag gaacccaggc
     2161 acttgccgta gcgcgccttg atggcctttt cggcgtcggt ggtggggttt tccgggaagt
     2221 cggggatggg gtagcccttt tcctggagtt ccttgatgca ggccttgagc tgggcgacgg
     2281 aggccgagat gttgggcagc ttgatgatgt tggcgtcggg ctggagggtg atcctgccca
     2341 gttccgccag ggtgttgggc atcttcagct cgtcggggag catgtcggag aactcggcga
     2401 tcacgcgggc ggacacggag atgtcgctct tgacgatgtc gacgccggcg ggcgcggtga
     2461 aggtgcggac aacgggcagg aaggcacagg tggccagcag cggcgcttcg tcggtgagtg
     2521 tgtagatgat cgtggatttc ccagcagcca ttgtttcttc cctcgatttg attgatttgg
     2581 gtgggtcgat ttcgaacgaa cgccctgttg catgccatgc tttccggtgc gcccacgcgc
     2641 cgaccggacc cggcacaagc ttagaagtat cgcgacccgt cgcaggtccg tcaaccgttg
     2701 cagcggcccc cgaaaaccgc gccgcggccg ggcgattgcg tgccggccgc ccggcttccc
     2761 ggagcgcctc cggttggggc ggaaaagggc gccgcgcgtc ccgctccgcc cccttttggt
     2821 gctgcgcgcc gggggccggc agcactcaga aggcgaattc gaggatgccc ttgaacgtca
     2881 gcttttcggt gtcggtggag aggcccttgc cgacgcccac attgaagccc cagccgtggc
     2941 gctccacgtc cagcacggcg aagagcttgt tgctctgggc gttccagggc tggcgctcgc
     3001 ccaccttgcc ctggtcggtg tagtactcga agccgccggc gaggccttcg ccgatgcggt
     3061 gggtgccctt gaaggcgaaa ccgtagccgg tgcgccggtc agccccgtcc gacagatccc
     3121 agctcagcac cgggttgaag gcgagcagcc agtcgggcgt gcgccagccg agcatggcgc
     3181 gcagctcggc ggcgctgcgg tattcctcga agcgccgggc gatccgcgcg atctccaggt
     3241 tgaggccggc aaaaccgccg ccctggagtt cgtcgccccg gatcgggagc cacttgaagc
     3301 ggcccttggc gccggccgcg tagaggttgc cggtcgccgc gtcgcgggcg gtgggcaggt
     3361 agaggcccgc gtccatcgtg tcgttgagcc cccaggagag ttccggcgtg acgaacaggc
     3421 tgtggtcgtt cttcacctcg cccgcgtagg tcgcgtcgcg ctggccgcgg ggcgaggtat
     3481 tcacgtgcac ttcgaggccg aattctcccg gtgcgttgag gtcgtcggca tagacctgga
     3541 tttcgtcagg cagggcggcc caggccgcgg gcagggcggt cagaccgagg agtgcgagga
     3601 gtgcgaggca ggccgaaagg cggcgggcgg tcatggggca gggacgagtg catacgacac
     3661 gggaggatag cgaatcgcgt gccgggctgc atccggggcg tgcggtgctg cgtaggaggg
     3721 tcaggggtgg cccttccggc cgccgttttc atcagggaga tgaacatgag attcagggtt
     3781 cggattgcgc ccatcgtgct ggcctggggg gcggccatgc cggccttcgc ggccagcgac
     3841 aagctgctgc tcactggcgg cgtgtcgcag ctcgaaggcg cggccggcgg cggcctcacg
     3901 ccgtgggcgg tgatcgccgg ctacggcacc cgcgaccaga ttggcgccag cgccttctat
     3961 acccaggtgc gttcccagga ctacgggctg ggcgtgtccg gcatcgcggt gggcatccgt
     4021 gatcgcctgg agctgtccta tgcgcaccag tccttcgata cccgcgacgt gggcggcgcc
     4081 ctggggctgg gcaacggctt cagcttcaac caggagatct tcggtgccaa gctgcggctg
     4141 ctgggcgacg cggtgctgga gcaggattcc tggctgccgc agatcgccat cggcctgcag
     4201 cacaagcgca acgaccaggg ggcggtgctg catgccatcg gtgcgaagaa ggaccacggc
     4261 accgacttct acgtagcggc caccaagctc ttcctcgacc agagcctgct ggccaacctg
     4321 accctgcggg agaccaaggc caaccagttc ggcatcctgg gcttcggcgg cgatctcaag
     4381 gaccgctaca cgacccagtt cgagggctcg ctggcctacc tgttgcgccg cgatctggcg
     4441 gtgggcgtgg agtaccggac caagcccgac aacctgggca tcgcccgcga ggacaaggcc
     4501 tatgacgcct tcctggcctg gcagccggtg aagaacgtgg cggtgacgct ggcctacgcg
     4561 aacctgggca acatcgtcat caaggacaac cagcgcgcga cctatctgtc ggtgcagctg
     4621 ggtttctgat gacgcccgcg attttccgga aaggaatcga acgatgaaga cctttagctt
     4681 ctgcgcagcc ctcgcgctcg ccctgatgcc cgtcgcctcc catgccgagg ccgatctgtc
     4741 cacctatcgc gcgttcggtg agaagccggg cctcgtcagc ctcatggacg ccttcatggt
     4801 caatctgctg gcggacggcc ggacccgacc cttcttcgaa catgccgacc agacgcgcat
     4861 caaggcacag ctggtggatc agatctgcgc ggtgctggat gggccatgca cctatcaagg
     4921 ggcgccgatg ggccccctgc acgctggcct gggcatccag caggggcact tcaatgccct
     4981 ggtggaggac ctccagcagg cgatggacgc acagggggtg ccgttccggg cacagaacaa
     5041 gctgttggcg cgtctggcgc ccctgcatcg ggacatcgtg acgcggtgag ccgtccggcc
     5101 gcctcagccg acgcggaaca cgcgtagcgg gccggcgctg tcggtggcga tcgccaccgc
     5161 gccggcgaag ccctggggca agggcgggaa gtgcaaggtc cagcgtccac gcagaccgtt
     5221 gacctcgccc ggcccgaccg ggctgggggg cgggaccaca ccgaagctgt cgaggtggcg
     5281 cgccaggccc attcccaggg ccttgagcgc cgcttccttg ccggtgcgca ggcgcatgaa
     5341 ctcgtggtta cgccggagcg gctcgctgaa gcggtcgatc cagctcagtt cgtcgctgca
     5401 cagggtgcgc cggaactgct cttcccggtc gtccagggga cgcacggctt ccaggtccac
     5461 gccgacgggc gccgagcggg tgacgccgat gagcgccagg gagtgggcat gggagacgtt
     5521 gaagtggagt cccgggtcct cttccagcag cggcttgtcc caggggttga gggggaagtt
     5581 cagggcattc ggcgacttgg acaggtaatg tcccagcagg atgcgcagca tgccgcgggt
     5641 gtacagctgg cgcaggcggt cgtcctcgaa gggcaggcgg ccggcgcggg cccgttcgtt
     5701 ggcggtggtg gcgctctcca cggcgcgaca cagggccgtg tcgcggatcg ggagctggac
     5761 ctgccagaga tgcacttcgc cgtccaccag cgggatcggc gcctcgcggc ccttggaggg
     5821 gcggcacagc agcggctccg ggaccgtgat ggacgggtag ggcgcggcgt cgtcgtgttc
     5881 tgtggcagtt ggtgcggcgg gcatgggccc cgatcccccg atggatccgg ccgggtgatc
     5941 tggccagatg atgtgtgggc agcagaatac cgcccacggc gagaggggcc cccattcaca
     6001 tcttaaaatc ttgtaatgtg attatgctta cagcgtatga ccggcgcagc cagggggcgt
     6061 attgctcttt ggcggtggcg gtgaggcgcc gggatcaggt cgccgcggcg aggtggctgc
     6121 ggatcgacac ggcgagcgcc tcccgcaggg tctggcggcg ctgggcgtcg gcgcgcttgc
     6181 ctgacgggaa gtcggagatc gggcgcactg gcggaacgac cgggaagtcg aaatccttgt
     6241 ccggccgcgg tgtgccgccc agatcggccc acagggcgtc atagtcggta tggatggtgc
     6301 ggcgtttgcg ccagctgcgg tagatgtgat ggtcattgcc caccgccacc agccccgtca
     6361 ggccgaagtt ctcggccagg ttctgggcga tcagcaggat cgccgccttg gggcgcaggc
     6421 cccacatggc cttggtggcg gccttgatgc gggcgctgcc gttttcaccc tggcccgggc
     6481 cctgcatgca gccgacggag agcaccggcc cgccgcgact gccaacgatg gcggcggcaa
     6541 tcgagtacag gcggccgtgt tcgtcgtgga gctggatgac gaactcccct tccttcatga
     6601 actgctcctc gcttgtgagg accgcccgga acacgctgtc ctgcttgccg aggatctcgc
     6661 cgacaggcac ggtccgcccg gcggcggcat ggagcagcag cccgccctgc gggtgatcca
     6721 ggcagaagcg gtagtgggtg ctcagggccg cgagacggcg tgacggcgag taatggcagc
     6781 gcaggtaggg gcggtggatc ttctcggcga ggtgggggtt gaggcgcacc agatgctgca
     6841 ggcgggcgtc gctctcgagc agctcgaacc agctgcgggt cggcccggca aagatcaggc
     6901 tgcgcaggtg gaagcgggtg gcttcgccgg cgcgccccgc cgcggagggc gggtagtgct
     6961 gggcggcgag atggcggatg cgggtggcga agggccgccc gcccacggtg cgggcgagga
     7021 ccagggcgct gcgccggaga aggcggtgaa ccagcttcat ggagacgatg gactcaggcg
     7081 gaggccggca ggctgacccg gacgagggtg ccctttccgc cagggggggc ggcgatgagg
     7141 gcgcgcccgc caaaacggcc ggcgatctcg cgcacgatgg cgagccccag accgctgccc
     7201 ggcgcggtgc tggtgggcag gcgatagaac cgttcgaaca ccagttcgcg cgcttctgca
     7261 ggaatgcccg gtccgctgtc ttccacgtcc acctccgcat gatcgctgct ctggcggcag
     7321 cgcacagtga cttcacccgg ggcgggagta tacgtgatgg cgtttatcac cagattggca
     7381 atcagctcgc ccagcagtaa tccgtcaccg cggatgcgca cggggccgag ttcgaagccc
     7441 agatcgatgc ccttggcttc cgcgagacgg atccagtcgg gggagcgctc gcggatgatc
     7501 tccggcacgt ccaggaggct gccggcagcg gcgtcgcggc cgcccggctc ggccgagatc
     7561 aggaccagca tctggccgat gaggtggcca aggcgttcaa gggccgcaat gcactgggtg
     7621 gtgcggttgc ggcgctgctc cggatcggcg tcgcgcagca gcagctcgag ctgggtacgg
     7681 atgccagcca ggggcgtgcg cagctggtgg gcggcgttgg ccacgaagcg ctgctgggca
     7741 tcggaggccc gctccaggcg gtccagcacg gcgttgaact ccaccaccag ggggcggatc
     7801 tcggcggggg cggagagggc atcgaggggt tgcagcgcgt ggtgttccat cccgtgcagg
     7861 ctgcgccgga tgtcctccac gggcagcagc gcgtggcgga tgccgaacca gatcgtgaag
     7921 ccggtggccg cggcaaagaa gaacaggggc atcgccaggg cgaaggccag gtccacggcg
     7981 agctggttgc gcttgcgcag ggtttcggcg gtgacgaaga tgaagggctc gcccgcaacg
     8041 ctgtggtgaa cggcggtgac gcggatcggc tggccgcgga agtgggagtt gaagctgacc
     8101 cgttcgccgt ggatctcctc gccttcgggc tggtggggca ggcccgcgtc gccggccacg
     8161 aagcgctcgc cggggcccag cacgaggtaa tagatctggt ccacccgatc cgcccgcagg
     8221 acctgctcgg ccgcaggcgg aaagacgaag tgcggctcac cgttgcgtac ttccaggtgg
     8281 gggaccagcg ccaggctggc gtccgccagg gcccggtcga gggcttcctc ggccggctgg
     8341 tgcaccaggt gataggccag gggcaggaag gtgaggacgg cgagaccggc aggcaggagc
     8401 agccagagca gcagggattt acgcaggctt gggctcatcg cgcaggtttt cgagcatgta
     8461 gccgaggccc cgcacggtgc ggatgcggat gcccgccgtt tcaagtttgg cccgcaggcg
     8521 ggagatgtag acctcgacgg cgttctcgct catgtcctgg tcccagcggc acacggccgc
     8581 ggcgatgcgt tccttgctca tgacccgccc ctggttgtcg agcaggaaga gcagcacggc
     8641 ccattccctg ccgggcaggg tcaggggctc gccgtcgagc caggcgcgat aggcctcctc
     8701 gtcgaggatc agcggcccga actcgcgtcg ggcaccccgc tggccacggg agcgtcgcgc
     8761 cagggcgttg acccgggcga ggagttccac cgcggcgaag ggtttggtca ggtagtcgtc
     8821 ggcgccggcg ttgagaccgc gcacgcgctc gttgagggcg tcgcgggcag tgagcatgag
     8881 cacatggatc gcactgctgc gggcgcgcag ccggcgcagc agctccagac catccatgcc
     8941 gggcaggccg atgtccagca ccagcaggtc gaaggcttcc gcagcgaggg acgcttcggc
     9001 ctgttcggcg gtgcccacgt agtcggtgac gaatccggcg tcgcgcagga cctgggcgac
     9061 accgtcggca agaaggggat cgtcttcaac gatcaggact cgggcgttca tggttcgggg
     9121 gcttcactcc aggggccgga tgcccggcac gtcattcctt gtcccgcagg ctctgggcgc
     9181 cgccgaagct gagcagcacc ggcagcagca acagggtgaa tagggtggcg ctgacgatgc
     9241 cgccgacgat caccaccgca aaggggcgct gggtttccga gccgatgccg tgggacaggg
     9301 cagcgggcag caggccgagg ccggccatca gggcggtcat gatgatggga cgcaggcgca
     9361 gtacggcccc ttcgaggatg gcctcggccc ggctggcgcc gcggcgggag atctcgatga
     9421 cctgctccac catgatcagg ccgttctgca ccgagatgcc ggccaccgcg atgaagccca
     9481 ccgcggccga tacggacagg tgcagccccg ccagccccag cccggcgaaa ccgccgatca
     9541 gggtgaaggg caccatggag agcaccagga aggctgggcg tacgtcccgg aaggcccaga
     9601 agagcaggga gaagatcagc cccagggtga tcggcacgat gaccttcagg cggcccatgg
     9661 cgcgctgctg gttctcgaac tggccgcccc aggtgatcgt gtagcccttg ggcaggctca
     9721 cctcggctgc gaccttggcc tgggcttcgg cgacgaagct gccctggtcg cggccgcgca
     9781 ggttggcctt gacgatcacg ttgcggctgc ccgcctcgcg ctggatgcgg gaggcgccct
     9841 ggcgcagctc cacatcggcc agatcgccca ggggaatgct gcccacgccg tcggcgaggg
     9901 gcaccggcag ggtcgcgatg tcgtccacct ggtcgcggaa ggctgcgccg aagcgcaggg
     9961 tcacatcgta gcggcggtcg ccctcgtagt aggcattgac cgcgctgccg cccagggcgg
    10021 tctggatcag cgcattcacg tcggtgacgt tgatgtcgta gcgggccagg gcttcgcggt
    10081 ggaggcggat gtccacctcg ctctggccgc ccaccggcag ggcggccacg tcggccgagc
    10141 cctggatgcg gttgagaatg tcggcaatct ggcggccctt ggcttcgagc acctccagat
    10201 tggggccgaa gaccttcacc gcgatctcgc ccttggcgcc ggacagggac tcttccacgt
    10261 tgtcctggat cacctgggag aagttggtgg gcaggccggg gatggtggac agcctggcgg
    10321 ccatgctgtc caccaggtcg tccttgtagg ggaagcgcca ttcgccgcgg ggcttcaggt
    10381 ccaccaggat ttccatgttg ttggggccct tggggtcggt cccgtcatcc gggcgcccca
    10441 cgtgggtgat caccgctttc acttccgggt agctggcgat gatggcgcgc accttgcgtt
    10501 ccacgtcctt ggtgttcccc agggctgagg actggggcag gctgatggtc agccagatgt
    10561 tgccttcgtc cagtttgggc agaaattcgg agccgagcat cggggccaac ccgagggacg
    10621 tgatgagcag cgcgatggag ccgacgacca tgctccggcg acgggcctgc agtcccagca
    10681 gcagggaccg gtagttctcc tgcaggcggt gcagccagtg gctctccttt tcggccatgt
    10741 ccttgttgag ggcgaaggac agcagggtcg gcaccagggt cagggtgagg atcaggccgc
    10801 ccagcagggc gaaggacagg gtcagggcca ccggcgtgaa gatccgcccc tcgacccgct
    10861 ggaaggtgaa gatgggcagg aaggcgacga tgatgatggc cttggagaac aggatcggct
    10921 ggcccagctc cacacaggtg tgcttgaggg catacaggcg tcgtccgacc ggcgagctga
    10981 tgtggtgttc cgccttggca tccaccgcga ggcgcaccat gagggcctcc accagcacca
    11041 cggcgctgtc gatgatgacg ccgaagtcca ccgcgcccag ggagatcagg ttggccgaca
    11101 cccccatcgc gtgcatcagg atgaaggcga acatcaggga cagggggatc accgcggcga
    11161 cgatcaccgc gccgcgcagg ttgcgcagga agaccagcag gatgccggtg accaggaagg
    11221 cgcccaccag caggttctcg cccacggtgg cgacagtgtg gccgatcagc tcggtgcgct
    11281 ggtagatcgg gcggatccgg tagccctcgg gcaggcgcgc gttcacctcg tccaccttgg
    11341 cgcgcagggc ctccacgatc ttggcggcgt tgccgccctt ggtcatctgc acgatgccct
    11401 cgatcacgtc gtcacgatcg ttgaaggcca cgatgccggc gcgcgggcgg gcgcccgagg
    11461 ccacttcggc cacgtcgctg atgcgcaccg gtttgccgtc gcgggccacg atgacgacgt
    11521 cgcgcacgtc atccagggtg ttgaacaggc ccagggcgcg caccaccagg gcctcgtcgc
    11581 cgcgccgcag gagaccgccg ccgccgttgg cgctggcggt ggcgagggcc tggctgacgt
    11641 cattgaggct cacgttgtac ttgcgcagca ggtcggggtc gatgcgtacc tggatttccc
    11701 gcaccgcgcc gccgaagctg accacgtcgg cgatgccggg cacgatgcgc agggccgggc
    11761 ggatgctcca gtcctgcacc gcgcggatct cctccagcgg cgtatcgggc ggcgactcca
    11821 gcacgtagcg gtagatctcg cccaccgcgg tggtgagggg cgccagggtg ggctggatgg
    11881 cgggcggcag gttgaccgtc tgcagacgct ccagcacctg ctggcgggcg aagtagtcgc
    11941 cggtgttctc ggagaaggtg agggtcacca ccgacaggcc ggtgatggac acggagcgca
    12001 gctgggtcat gcccggcacg ccgctcatct cccgctcgat gggcagggtg acggtgcgct
    12061 ccacctcttc cggggcctgg ccgggcacct gggtgacgac ctggacctgc acgtcttgta
    12121 cgtccgggaa ggcctcgatg gaaatctgac ggaaggcata gatgccatag ccgagcagcg
    12181 ccagggcggc gatcagaaaa aaggcccgct gacgcagggc ggcggtgatg agggcgtcga
    12241 acatggcgct taccgggccg agctgctggc cagttcggcg ttgaggagca gtgcaccggc
    12301 gctgaccacc ttctcaccgc ccttgaggcc gtgccagacg taggcgaact ggcgggtctg
    12361 caccgcgagg gtgacctcgc gcttctcgaa ttcgcccggg gcccgttcca cgaagacgaa
    12421 gttcttgacg ccttcgacca gcagcgcggc cgtggggatg cgcggcagtt cctcgcgggc
    12481 gtcggcgagc agggagacgc gggcgtacat ctcgggcttg aggagctttt cccggttgtc
    12541 cacgctcacc cgcgcctgga cgcgacgggt ggccgggtcg agggtggggg ccacgtgcac
    12601 cacctgtccg tcaaaattgc ggttggggta ggcggaggct tccactacgg cggccttgcc
    12661 cacggtgacg aggggcagat cgcgctcggg cacgtccacc agcaccgaca ggcggctcag
    12721 gtcggtgacg acgaacaggg gatcctgggc gtcagggcgg acttccatgg cgggattgat
    12781 gtgccgcgcg gcaatgacgc cgccgatcgg cgcgcgcacg gtgtagcgct gaccggtgat
    12841 cgcgccgccg ccggggctca ggttggtcag ccgcaggcgc gcccggtctg cctcggcgcg
    12901 ggcctggcgc cagtcggact cggcggattc caggtcgcgt ctggcgatca cttccgcttc
    12961 gaacaggctg cgggcgcgct cgaaggcggc cttggtgcgc gaggcctcgg catcggcctt
    13021 gctcacgtcg gcctcggcgg cgcccagctc gggggcgtcg agcaccgcga ggacggcgcc
    13081 ggccttcacg gtgtcgccga tctgcccgtg gatctcgatg acgcggccgg ccaccggggc
    13141 gaacaggcgg gcggtggcgt tctcgtcata ggccaccttg gcggccaggg gctcgtccat
    13201 gggcaccggc gagacggcgg cggcgtccac cttgagctgg gcgagctgcg gcgcggccgt
    13261 ggggaagcgc agcaggccgg gttcggccga gggcttctgg acgaccgggg tgtcgggcgt
    13321 gtcttccccc acgcgggtga tgcggaggta gacggcggcg gccacggcga tggcgaccac
    13381 ggcggcaatg gcgatctgct tgcggttcat ggcgtgtctc cggccagcgc cagggcctgg
    13441 cgggcgcggg ccagatcggc ccgggcctgg atggtttcga tgcgggcggc gcgcaggccg
    13501 cgctgggtat ccaggtagtc ggtgaggctc atgccaccct tctggatggc gacctcggcg
    13561 ccgcgggcgg cccgctcggc ggcgggcagg gcgatgtcct ggaggcgccg gtagcgggcg
    13621 tcggcggcgg cgacctcggc cttgaggcgc aggcgttcgc cgtccacagc ggcgcggatg
    13681 cgggcgagct cctgctcggc catcaggtag tcggcttcgc tgcgggcgat gtcgccgcgg
    13741 tagtcgtagc cgaggaagag cgggacgctg acgctgaggc cgaaggtatt ttcggacacc
    13801 gcgttgttga ggctggccgg cgtatgttcg tattgaacgc caacggacag gtcacgactg
    13861 cgctgggcgc gggccagccc tcgcaatgcg tctgcctgtt caaggcggcg acgggcggca
    13921 gcgatatcgg cgcgctcggc gttggcggcg acggaattcg tcgcctcgtc gggccaggtg
    13981 tcggcggcgc gcagttggtt gggggcgatc cggttgccca ggatctggga gagagcgata
    14041 cgcgcctggg ccaactgcaa accggcctgc acagcgtcat tggcgacgcg ggcggcatcg
    14101 gcggcgtaac gggccacgtc cacaccggcc agatcccccg ccttgccgcg cagttcggcg
    14161 gcctgggcgg tacgcgtggc aaaggcggca ctctcgtcgc tggcccgctg aacgtcctgg
    14221 gcagtgcgca ggtcgatcca ggcgttggcc aggtcgatgc gggccaggcg gatggtgttg
    14281 gtgaggtccg cgtcggcagc ttgcacaccc cgttccgcag ctttcaggcg cagatcgcgt
    14341 ttgtcgccgc gctcgaaggt ttgatcgatg cggacggagt tgtccgtcgg tcgacgccag
    14401 cggctgccgc ccgtgtcgat cgtgctgaca cccagcgtga gctgcggatt ggggcgtgca
    14461 ccggcgatgt cgataccggc gtgggctccg gtcagttggt ggcgggcctg caggatggcc
    14521 ggattggcgt ccagcaacag gcgatcggcg tcggccagcg tcagggggct cacggacgcc
    14581 tgggccaggc acagggcggg caccatcagg gccgccgcga aagccggggt aaagaggcgg
    14641 ggcatgcgaa ctcgcaacga ggatcaggcc cgcagtgtag ggcgcctctc tgtcgatgtg
    14701 ctgacgaaat ccttgcgcga atcttgcgtc cggggcgttc gccgtggcgg acgcagtctg
    14761 cggcgtgtca ggcgcaggcg accgggtcca ggacgctgac catggcttcc accagcaggc
    14821 agatgtcccg ccggtcgcgg gcgtccacct cgaagagggg catgccgccg tgaccggcgt
    14881 agctcatcac cgtgtggaac aggtcgatgc gcgggcgcgg ctgctggtcc atgtgggtga
    14941 tgccgacggc gaccgcggtg cgccggatga aggcgtcgaa gctgcgcagg tggcgcagca
    15001 ggtcgtcgtc gggcgcggtg cggcggttgt cgatgagcag gatcaggccg aggctgtcgt
    15061 cgccgctgac gccggtggcg gtctcgcagg cgctgctgca caggtggatc acgccgccgt
    15121 cgtcgagggt catggcgccg tagtcggcgt tgcctttcca gatggccggg atgtcgctcg
    15181 cgacgtcgat ggccgaggtc ttgcccgctc cggacggtcc gctgaagatg atccgtccgc
    15241 tcacgggcgc gggtccggcc gctcgtcggg gcgggcgccc aggaggcggc cgagggtgcg
    15301 ctgcagcagc ccgcgccgtt ccggcgtccg ggccggatcg cccgtgccac ggcggtccgg
    15361 cacgccctca tcgccgagct gcagcaggtc cagggcggcg caggcgctga agaaggcaaa
    15421 gacgtgacgc tggggcaggc cgagcagatg cacggtatcg cgcagggtgg tcggcgtgcg
    15481 cgaccacagg ccggcgatgc gcagggcgcc ctcgggcacc ggcaggcggg tgaagttcgg
    15541 ccagcgcacc agtcgcaccg gggtgtccag gtcggtgccg cgcggcaggc gaccgcggga
    15601 ggcccacagg gctacttccc actcgacgac ctgcagcggt cgcgggtcga ggcgggcgac
    15661 ggggcccacg tcaccggcgt cgagggtggt ctgcttgatg gtgccgcggg tgggcacctg
    15721 ggcctgggcg cgcagggcct tggggctgag gggctggtag gccgactcgt tggtcgggtc
    15781 gagatagagg atggtgccga gggggccgcg cagcttgacg gggcgttcca cgtcacgggc
    15841 gtgggcgagg gcccgtgcca gcgtgccctg caggaagttt tcggggaggt agtagatgcg
    15901 ttcgcgctgg cgggggtcgt ccaggtccac gtcgggtgcc gagcccacgt agcgggtggc
    15961 gaagatcgtc tcggcttcga cggcgtcggt gaaggccggc aggtcgtcgg gcggggcgcc
    16021 cagatcgccg tagaagggcg gctggggggc gcggcgaccg tcgtcgcggc tgcgcggcag
    16081 gagttcgtgg acccgctgca gggcggccga gaatgcggcg agccccaccg gcttggtcac
    16141 ctggatggtg aaggggtcgt ggttgggtgt gggacggctg gaggtgacga tcatcggccg
    16201 gcccgggtag agcaggcggt gaccttccag cagatactgg cccaggtcgc cgtccgcgtc
    16261 gatgaaggcg gcgtcggcgt gattctcgtc ggtgatgcgg caggagaagg catccacgcg
    16321 ggacaggaag atctggaagg catccagggt gcggccctcc atgccggcat ggcacaggcg
    16381 cagggcggaa cgggcagagg tcatgacacg catccttctc ggttgggtgc cgtctgccgc
    16441 gagggccggt gtgaccggtc cgtcggagga ggcggggcgg agggagcgcc cgtgacggct
    16501 ttgcgggaat gataggtgga tcaccgccgg ccggatgcct tcgcattggc aacttctgtc
    16561 acgttaacag tgttttacaa aaccatattt aattcatggg tattccgccg cctagggatg
    16621 ggcggcagag gggcgtgaac cgcggagcga cgggttgtca cggcctgccg gcgagggcgc
    16681 ggcccttgac cagcagatag agcgccggga tgacggcgag ggtgagcacc gtggaggaga
    16741 gcatgccgcc caccatgggc gcagcgatgc ggctcatcac ctcggagccg gtgccacggc
    16801 tccacaggat gggcagcagg ccggccatga tggcgacgac ggtcatcatc ttggggcgca
    16861 cccgctccac ggcgccggag gtgacggcgg cgaggaggtc ggcgcggctc gcctcgcggc
    16921 cctcggcgcg gcagcgggcg agggcgtcgg cccaggcgtg gtccaggtag atcagcatca
    16981 ccacgccggt ctcggcggcg acgccggcca gggcgatgaa gcccacgccc accgccaccg
    17041 acaggttgta gccgagcgcg gccagcagcc acaccccgcc caccagggcg aagggcaccg
    17101 acagcatcac gatgaaggtc tcggtgaggc ggcggaagtt gagatagagc agcatgaaga
    17161 tgctcagcag ggtgatcggc acgaccacct gcagacgctc gcgggcccgc tccatgtatt
    17221 cgaactggcc gctccaggtg acgtaggcgc cgggcggaaa gtgtacctgc tcggcgacgg
    17281 cgcgctgggc gtcggccacg tagccgccga tgtcccggtc gcgcaggtcc acgtagagat
    17341 aggcggcgag ctgggcgttc tcggtgcgga tgcccggcgc gccgcgggcg atggacaccg
    17401 tcgccacctg gccgaggggc accatggcgc cgccgtccac cggcaccagc acctcgcggg
    17461 cgatgcgttc cgggtcgctg cgcagctcgc gggggtagcg cacggccacg gcgtagcgct
    17521 cgcggccttc cacggtggtg gtgacggcct cgccgccgag ggcgccggcg atcacctcct
    17581 gaagctcacc cacggacagg ccgtagcggg ccaggcgcgc ccggtccggt tcgatgtcca
    17641 gatagagccc gccggtgagg cgttcggcga aggcgctggt ggtgcccggt acctggcgta
    17701 cgacggtctc gatgtctttg gcgaggcgct ccatctcgtc caggtcgcgg ccgaagacct
    17761 tgatgccgat gggcgtgcgg atgccggtgg cgagcatgtc ggtgcgcgcc ttgatgggca
    17821 tcgtccaggc gttggcgacg ccgggaaact gaagcgcctt gtccatctcg gcgacgaggc
    17881 catcggtggt gaggccgggc cgccactgct cgtggggttt gagggtgatg accgtctcga
    17941 acatctcgac gggtgccgga tcggtggcgg tgccggcgcg gccggccttg ccgaagacgc
    18001 tgtccacctc cgggaagctc ttgatgatgc ggtcctggat ctggagcagc tcggcggcct
    18061 tggtgaccga caggccgggc agggaggtgg gcatgtagag cagggtgcct tcgtccaggg
    18121 gcggcatgaa ctccgcgccc agacgggtgg cggggatcgc ggtgagggcc agcaccgccc
    18181 cggcgaggac gacggtgagg cgcggccacg ccagcacggc ggcgatcacc ggccgataga
    18241 cgcggatgag caggcgattg accgggttgc gggcctccgg cagcacccgg ccgcggatga
    18301 acaggcccat cagcaccggc accagggtga cggagagcag cgctgcgccg gccatggcga
    18361 aggtcttggt ccatgccagc ggggcgaaca gacggccttc ctggccttcc agggtgaaca
    18421 ccggcaggaa ggacacggtg acgatcagca gggagaagaa cagggccggt cccacctcgc
    18481 ggcaggccgc cagcagggcg gcggcccgcg ccgccggggg cgcgtcgtcc ggcaggcgtt
    18541 ccaggtgctt gtgggcgttc tcgatcatca cgatggcggc gtcgatcatg gcgccgatgg
    18601 cgatggcgat gccgcccagg ctcatgatgt tggagttcat gccgagcagg tggagggcga
    18661 cgaaggcgat cagcacgccc accggcaaca tcaggatcgc caccagggcg ctgcgcacgt
    18721 ggagcaggaa gaccacgcag accgccgcga cgatgaggga ttcttccagc agggtgtggc
    18781 ggagggtggc gatggcgcgc tggatcagct cggagcggtc gtacaccgtc ttcagcgtga
    18841 cgccatcggg caggccgggc gccaggtcgg cgagggtggc cttgacgtgg cggatcacgt
    18901 ccagggcgtt ctgcccgctg cgcgccacgg tgatgccgcc caccgcctca ccttcgccgt
    18961 ccagctcggc caggccgcgg cgctcgtccg gcacgagttc cacccggccg atgtcgcgga
    19021 ccagcaccgg cgtgccccgt tccgccttga ccaccaggct ggcgatgtcg tccacgccgc
    19081 gcaggtagcc gcgcccgcgc accatgtatt cggtctcggc catctccacc acgcggccgc
    19141 ccacgtcccg gttggaggcg cggatcacct gggcgaccct ggacaggggt atgccgtagg
    19201 cgcgcagctt caccggatcg acggtgacct ggtaggtctg cacgaagccg cccaccgacg
    19261 ccacctcggc cacgccgcgg gccttggcga ggggatagcg cagggtccag tcctgcaggc
    19321 tgcgcagttc ggcgagggtc ttgtcccggg ccagcacggc gtactggtac acccagccca
    19381 cgccggaggc gtccgggccg agctggggca tgatgcccgc gggcaggcgt ccggcggcgg
    19441 cgctgaggta ttccagcacc cgcgagcggg cccagtagag gtccgtgccg tcctcgaaaa
    19501 tgacatacac gaaggacacg ccgaagaagg aaaacccccg caccacgcgg gattttggca
    19561 ccgacagcat ggcagtggtg agggggtagg tgatctggtc ctccaccacc tggggcgcct
    19621 ggccggggaa ctcggtgtag acgatgacct gcacgtcgga gaggtccggc agggcgtcga
    19681 tgggcgtgcg cagcacggcg tagacgccgg cgaggaccac gcagagggtc gccagcagca
    19741 ccaggaagcg gttgcgtccg gaccagtcga tgagggctgc cagcatggtg tcgccctcag
    19801 cggccggcgg ccgggcgcac cgcggtgatg acccactcgc cgggcttacg ctcgacgaag
    19861 tccaccgcca cccgcgcgcc gggcttgagc gcggcctgca gggccgggtt ggcgacggcg
    19921 aagtccatgg tcatggccgg ccagttgagg ctggccaccg ggccgtggct gagggtcacc
    19981 gtgccggccg cggcgtcggc gccgtccacc tgcccttcgg cacgatgcac cacgctcgcg
    20041 gcctgggcgg ccggcggcgt gccgccggtg gccgtgaagg cgcccagcgc ggcctgcagg
    20101 ttgctctccg cgtcgatgag gaagttggcg gccaccacca cccgctcgtc cgccttcacg
    20161 ccgtcgagta cctccacgta gcgggcgctg cgggcgccca ggcgcacctc gcggggttcg
    20221 aagcggcctt caccccgttc caccagcacc acccggcggg tgccgctgtc gaggaccgcc
    20281 gcgctcggca cggtgagtac cggcgcagcg ctgccggcgg gcaggctcac gtggacgaac
    20341 atggccggct tgaggcgcag gctcggattg gccagttcga tgcgcaccgg cacgctgcgg
    20401 gtgtcggcgt tgagggtcgg gtagatgtag ctcacgcggc cgtcgaaggt ctcaccgggg
    20461 taggcgtcca cccgcagctt caccgggctg ccagggcgga ctgcgccgat gtcctgctcg
    20521 ttcacgtccg ccaccaccca cacggcggac aggtcggtga cctggtacag ggtctcgccg
    20581 ggcatgaagc gcatgccctg cacggccttc ttctcggtga cgatgccggc cgccggtgag
    20641 gtgaaggtga gtgtccggcg ggcctcaccg ctgtgggtga gggcgcggat ctgggcgtcc
    20701 gggatgtccc agttgcgcag tcgctgaagg gccgcgtcgg cgagctggcg caggcctttc
    20761 tgggcctccg ggccggcgtc cttgaggctg tcggcgccct gggcggcgag ggcgtattcg
    20821 cgctgggcgg acaccagctc cgggctgtac acctcgaaca gggcctggcc gcgggccacc
    20881 ggctggccgg tgacgctcac gtgcaggcgc tccacgaagc cttcgaactt gggcgcgatg
    20941 gcgaccacgc ggcgctcgtc cggctccacc cggccgctgg cgtccacgct gcggtccagg
    21001 gcgcgcaggg tgacggcttc ggtgcgcacg ccgagcttct ggatcttttc agggctgagg
    21061 cggacctgtc cgggggcggc ctccgcgtcg gcctcgcctt catagaccgg gaggtagtcc
    21121 atgcccatgg ggtccttctt gggcaccggc gaagtgtcgg gcaggcccat ggggttgcgg
    21181 tagtagagca gcttgcgcgc ggccggggcg gccggggtgg gcgaggcggc cgggggttcg
    21241 gtgccgtggc gctggccgag tccgtaaccg gcgcccgcgg cggcggccag ggcaagaagg
    21301 gcggcggcga ggcgcagggc ggggcggggg gcgctcacag gtcttctccc acgaggcgtt
    21361 cgatgtcggc aaggcgcagt tgcgcctcgg tctgggcctt gaggcggttc tggcgggcca
    21421 ggcggatctg gcgctgggcg tcgagcaggg tggtgaagtc caccttgccg gtctcgtagc
    21481 cggccagggc ggcgcggaag gtgagttccg cctggggcag caggctctgg ctggccaggg
    21541 cttcggtgcg gcgcgcggcg tccagggccg acagggcctc gcccagctcg ccgagggcct
    21601 gctgggcggt ggcctcgcgg cgggcgcggg cggcggcgag caggctgtcg gcttcccgct
    21661 cctgggcgcg gcgcgaggcc tgctgcagcg gcaggttgag ttccaccatc agctcccatt
    21721 cgcgcacggc gttctggtac tggatcggcg agatgccgac ggtgaagtcg gggtagcggt
    21781 tgcgcagggt gagggcctgg ttgcgctcgg cggcagcgac gcgcgcctcg tcggcgaaga
    21841 gctggggatt gcgggcgcgc aggcggtctt ccagggcggc cggatcgaga cgcgccggga
    21901 ccggcagcgg gcgcaggcgc tccgctgcgg cgaggggcgc ggcggacggg cgggcgagca
    21961 gggcgttgag gcgcacctgg agctggcggc cttcgttgtc gagggcgacc aggtcggcct
    22021 gcaggccggt cagttccacc tgggcgcgga tggcgtcctg ctgcggggcg aggccgccgg
    22081 cgtagcgcac ctgggcgatc tgcccgatgc gtccgccgag gtccagcagt tcgcgggtga
    22141 ggcgggtgct ctggtgaacg tagtggagct gggtccaggt gatcttgatg cgcgcgctca
    22201 ggtcggccca ggtggcgtcg gcgcggccgc tggcgctgcg ggcgtcctgt tcggcggcgg
    22261 cctgcttcag gtcgcgcttg ccggcccagg gcagatcctg catcagcagg taacgggtgc
    22321 tgccggtgcg ggcggggagc agggcggggc tctgctcgcc catgcgggtg atgtcgcgca
    22381 gttcggtgcg gaagcgcgga tcgggcaggg cgccggcggg gacgacgcgc tcggcggcgg
    22441 cgtcggcctc gtggcgcatc gcggcgagtt ccgggctgtg ggcgcgggcc caggcgagca
    22501 gcccctccac gtcgcggccg aggggagcct cctgggcgca ggctgccgcc ggcagggcga
    22561 cggcgcatcc gataagcagg agatggacga ggcgcggagg cttcatggcg gcggatgatt
    22621 caggggcgtt cgatcttcac cacggtgagc gcaccgttga cggtgtcggc ggcaaagcgg
    22681 atgcgctcac cgggcttgag cgtgtccagc caggccgggt ccttcacggc gaaggccatg
    22741 gtcatgccgg gcatgccgtt gagggggccg tgggtcaggg tgacacggcc ggcggccttg
    22801 tcgatcttct tcaccacgcc gtcggtgagg gcctgggcgg cagtcgcgtg ggccgcgtgg
    22861 gcggtgggct cggcggcaat cgccggcagg gcggcgagga ggacggcggc gaggggggcg
    22921 gcgggcagca ggcggaggcg catggcagta tccggacgat ggatgacggg aatgtcgtca
    22981 ttgtgggcca tgggcgccga cgggaagctg tctgccggat tacaaatctg taagcttcgt
    23041 ccgcccgcac gatcctgcgg gacactggga ggcaaggggc ggcggggcgc ccgcgggaga
    23101 cgcacgatga agatcctgat tgtcgaggac gagagcaaga ccggcgacta cctgcgccag
    23161 ggactggtgg aagcgggctt cgtggcggac tgggtgcagg acggcctgga cgggctgcac
    23221 cgcggcctga cggaggccta cgacctggtg atcctggacg tgatgctgcc cggcgcggac
    23281 ggctggcagg tgctgcaggg cctgcgccgc gccggcaagg atctgccggt gctgttcctg
    23341 accgcccgcg atcaggtgga cgaccgggtc aaggggctgg agctgggcgc cgacgactac
    23401 ctggtcaagc ctttcgcctt ctcggagctg ctcgcccggg tgcgcaccct gctgcggcgc
    23461 ggcgcgcggg cgcgcgagcc ggacgtgctg aaggtggccg acctggagct ggaccggccg
    23521 cgccgccggg tgacccgcgc cggccggcgc atcgacctca ccgccaagga gttcgccctg
    23581 ctggagctgc tcctgcgccg gcgcggcgag gtgctaccgc gctccctgat cgcctcccag
    23641 gtgtgggaca tgaacttcga cagcgacacc aacgtgatcg aggtggcggt gcgccgcctg
    23701 cgcgccaagg tggacgaggg cttcacgccg cgtctgatcc gcaccgtgcg gggcatgggc
    23761 tacgtgctgg aggaaacgcc gtgggactga accggcgcct gtccctgacg gtccgcctga
    23821 ccctgctctt cgccctggcg tcgagcgcgg tgctgctgat cctcggcttc atcgtcggcg
    23881 cctcggtgga gcgtcatttc gaggagctcg accgggccac cctgtacggt cagctggcag
    23941 atctgcgccg cgggctggcc ctcggccacc ttcacgggga agcgggcggc gagggcggcc
    24001 tggtggaagg cgcccacctg catccgccga tgatggagct gcgcggtccg gacgggcggg
    24061 tgctcgccgc gtcgcccggc gccgtctttc cgtccgatgt ggtgatcggc gcgcccgggc
    24121 ctcaggtgtg gcaggacgcc tccgggcact ggttccgcgg cctggccgcc gagttgcccg
    24181 ctgcgccggc gggcgccggc ccgctgcggg cccgggtggc gacggacatc tcccatcatc
    24241 aggccttcat ggacggcttt cggcgcaccc tgtggggcgt ggtggtggcc agcgcgctgg
    24301 ccaccgggct gctcggctgg gccgcggccc gcagcggcct ggcgcccctc gaggcgatgc
    24361 gccgcaccgc cgcccaggtc agcgcccagc gcctcgaccc gcgcctggcg gtggattcgg
    24421 tgccggtcga gctggcggcc ctggcggaat ccctcaacgc catgctggcg cggctggagg
    24481 agtccttccg gcgcctgtcg gatttctcgt cggacctggc ccacgagctg cgcacgccgg
    24541 tgagcaacct gatgacccag acccaggttg ccctgtcgcg cccgcgcagc gccgccgaat
    24601 accgggaggt gctggaatcg ggcgcggagg agctggagcg cctgacccgc acgattgccg
    24661 acatgctctt cctggccaag gccgacaacg gcctcatcgt gccggcgcgg gaacccgtgg
    24721 agctggcgga cgaggtgcgt gccctcttcg agttctatgc ggcgctggcc gaggaggtcg
    24781 gcgtgagcct gtctcaggcg ggcgccgggc gcgtggccgg cgaccgcctg atgctgcgcc
    24841 gtgcgctggg caacctgctc tccaacgcgc tgcgccacac gccggcgggt ggtgccgtgc
    24901 gggtgaccct cgggaacgcg gaggaccggg tggtcgtcgc ggtcgccaat gacggcgaac
    24961 cgatcccggc ggaacacctg ccgcgcctct tcgaccgctt ctaccgggca gacccggcgc
    25021 gccgtcatgg cagcgacgga gacggcgccg gcctggggct cgccatcacc cgctccatcg
    25081 tgcgcgccca tgggggcgag gtggtcgtgc gcagcggcgt ccatggcacg gtcttccaga
    25141 tgcgcctgcc gccggccggc ggcagctgat ggcggcccag acgggcgtag ggcggcgaga
    25201 cgccgggcgg ccgggttcgc gcgagcacct tgcgatccac ttattgcggg gtcgtgtgca
    25261 cctcgcccgg ctgccagccg ccgcccagtg caaggaacag gcggatttca tcggttgcga
    25321 ggcggccacg atgcgcggcg aggcgcgcgt ccgcggcgag caggcggcgc tcggcgtcca
    25381 ggtcgtccag cacacccgta cggcccgcct gatacaaggt gtgcacctgg cggcgcgcag
    25441 tttcagcgtg ggcgcgggcc tgctgcagtg ctgcgtggcg gtcccggtcg cgggccaggg
    25501 tggcgagggc gctctcggtt tcctggaggg cgttcaggac gacctgatcg agggtcgcca
    25561 gtgcgccggc cgcgcccgca tcggcctggg ccacgcgggc gcgggcttct tctccgggga
    25621 ggctccagct cagcacgggg cccaggctcc agcgctgggt gggggcttcg cccaggtggc
    25681 tgaggatgcc cgtgccgccg gcgctcaatc cgaaggacag gctggggtag agggccgccg
    25741 tggccacccc gatgcgggct gatgccgcgg caagccggcg ttctgcctgg cgcacatcgg
    25801 gccggcggcg cagcagggcg gcgccgtcac ccacgggaat cggctgggcg agatcgggca
    25861 ggtgccggca ggcctcagcg gcagcgggcg cgcgggcggg ggactggccc gtgagcgcgg
    25921 caaggcggta ggcggcgctg cggcgacgcg cggcgaaggc gggcagggcg gcgcgcagct
    25981 ggtcttcctg ttctgcggcc cgcggcagat ccagcgggat gcgccgtcca ccggccacca
    26041 ggcgggcgat ggcctcgcgt tcgcggacct gcagtgccag ggcgcggcgg gcgagatcga
    26101 gcgcctcgcc ggccgcgcag gcgtccatgt aggccagcgc ggtgtcggcc accacactga
    26161 tccgggccag atcgccggcg gcttgcacgg cgccggcctc cgcctcggcg acttccaggg
    26221 cccgccgcag ttgtccggcc aggtccacct ggtagtgggc ccgcagaccc gcgttgccca
    26281 ggttgaagac ggggaccttg tgttcgagca gataggattc agccgaaacc tgagcgcgct
    26341 cgatcgcgac gccggcgccc agggacggat cgcgcaggcc gtcgacttcc cggaccacgg
    26401 cttcgctgcg ggcgaggttg gccgccgccg ccctgagccc ggcattggcg accagcgcgc
    26461 gcgcgatcag gtcgttcagg gcggggtcct cgtacaggcg ccaccaggcg tagggcagat
    26521 cgtcgcgacc gaagggcggc tggccggctt cttggaaggc ctggcgggcg tcggggctgc
    26581 gcagctgtgc agcgtcgggt aggcgatagt cgggcccgac ggcgctgcag gcactgagca
    26641 gcagcgcgat ggcgcccgag acgagggcgg cgcggggcgt catcgcgcgg ggccgccgtc
    26701 gcgttcggcg gtcggcggga ggagcgtcac cgtggccgtg cggccggcga tcagcgcagg
    26761 gtcgtggggg gcgtcgaggg cgatgcgtac gggaatccgc tgggccagcc ggacccagct
    26821 gaaggtcggg ttgacattgg gcaggaggct gctgccggac gtccggtccc ggtcctcgat
    26881 gccggccgcg atgctctgta cgtgccctgt caggatccgg tcgtcgccca tcaggtgaac
    26941 gcgggccgtc tgcccgacgg agatgcgggg cagcttggtc tcttcgaaat agccttcgac
    27001 gcgcagggag cgggagtcga tcagggccat caccggcttg ccggccgcca catagttgcc
    27061 gacgcgcagg gtgaggtcgg acagggtgcc gtctgccggc gcgacgatcc gggtacgctt
    27121 gaggttgagc gcggccagtt cccgctgcgc gcgggcctgg gcgagcgcgg cgcgggcctc
    27181 atccaggtgg gcctgggtct gttcggccgc ttcgcggctg accagttcgc cgagcgtctg
    27241 gttacggcgc acctcccgct gcagctgggc caggcgggct tcctggacgc gctctgcgga
    27301 ttctgcgtcg tgcaaggcca gggcataacg ttccgggtcg agctggaaca ggaactggcc
    27361 ggccttgacg ggctggtcat gaccgacggc gaggtccgtg acggtgcccg agacgtcggg
    27421 cgcaatctgt acgatgtcgg cgcggacacg gccgtcgggg gtccacggct cgctgcggta
    27481 gtggatccag agctgccggc cggcgatcag ggcgaccgcc acgatggcca gtgtgagcag
    27541 cacgggagcg gctttggaga ggaaggtcgt gttcacgggg gcgatctccg cggagggttt
    27601 cagggtgagt gcagcgccca ggcggccacc accagtcgcc agacgatgat gaacagcgcg
    27661 gtctcgaaga gcggtcgatg ccatacgcgg cggagcagtc cgagccacac gaggccccgc
    27721 tgcagcccga aggtgagcag cagggcggcg atgctggtgc tcagttcggc gctgagatag
    27781 acgccgaaca gttcgatctc gccgttcatg ggttcagtcc tccggcggca tagggcgtgt
    27841 ccgggaagag gttgcgccgc aatcccacga gggcggcaag ggcgcggccc tgaccggggg
    27901 ggcctccgag gtgccggatg cagccgtcga gcagcgcgca gaggtcctgc ggcgggcggg
    27961 cggttccgtc gggacgctgc tggtagtggc ggccgacggc gtggagcaat tcggtcacct
    28021 gcgtctgtgc gatcccgggc aggggcggat gggcgtcctg cagggtgctc aggtccatgc
    28081 cgatgcgcag ttcccgcagc acatcgccgt cggcgagctc ggcgtcttga tccctgaggg
    28141 ccgccaggcg gggtgccagc aggccgatcc ggtccaccag ccgggccgtg aggttctggt
    28201 gaggctcgcg gacgtctccg tgggcgaagc gcgccaggtc ctgccaggtg gcccgggcca
    28261 ggcggcgggc gctggacgcg gtactcaggg agcgggccgc gcgggtgacg taaatggcaa
    28321 cgaagacgcc gaagaactgg gacaggttgt tgttgaggaa gaccgcgaga tccggcgtga
    28381 agcgctcctg gatggccatg aaattgcaga agttgaggag gatgaagagg gccgacgagg
    28441 ccaggcgcgg gttgagtgtc gcggcgccga tggcgagcag cggcggtgcc aggacaaggg
    28501 cgaggagcgg gaaggtgttg atggccggaa acaccgcaaa ctggtagagc gcggcaaggc
    28561 tcactgcgat acagatggcg acgccgaact tgcggatggc cggtgcgggg tcgtcgaggc
    28621 tggcaaacag acagcacagg atcgcggcga gcattgcgct ggcaccgcct tcggaccagc
    28681 cgagtccgat ccacgcggcg cagcagatga ggatggcggt ggtggcgacc gcgcccgacg
    28741 tgagcgccag ccgccagtca ctgtgcagcg gacgtgcgcc ggcctggccg agggcgtccc
    28801 gcagggcggg cgcgaggggg gcgtccggtt cgtgcaggtg ggcgagcagc tgacggcatt
    28861 cggtctggat ctgcaccaga tcgagcaggc gtgtggtcag accgttccgg ttgagtgcgt
    28921 accaggaggc ggcgtcaggc gccgctgccg gtgtgtccag gtctgcggcc agcatggcgc
    28981 tgagcgcgcg gccctccgcc gcggggcagt ccgcgcgaat ccaggcaccg acgcgggtca
    29041 ggcggtcctg cgtgggcgga tcgacggggg cacgcatccc gtggagggcg gcgaggcgat
    29101 ccgagaggct ggacaggacc gggatcagca gcaggatgtg gtcgtggagc gcccggacga
    29161 tggcggtggt ttcgcgcaga tgggaggtgt cgaagggcag gtgcgccgcc aacaggtgga
    29221 tatccgtggc cgccgcagcc agatgggccc gatgcggaac gccggcgctc gggtcatcgg
    29281 cggcgaaaag gtcgagcgcc cagcggtcgg cctcaccgag ccagtcccgg atgcgctgct
    29341 cgagcacctc gcccaccgag cggggaaacc acaggctatg ggccagcgtg gcgcagagga
    29401 tgccgatgga gatctcctcg gcacgtgcca cggcaaggtc gaagatctgc ccgggttgat
    29461 tgacgctgct gaagccgacg atggcggccg tgtagccggc cagcatgagg acatagctgc
    29521 gcggtgagcg atccagcagt gaaatggaca ggcaggcgcc gacccacagg gccatcgctg
    29581 cgcacagcag ttcgggcgta ttggcgaggg cgggcagcag cagcaccgtc atcgcagcgc
    29641 ccagaagggt gccggccaga cggtagacag ccttggagcg tacggcggct gccaggggct
    29701 ggctcaccac gtaggcggac agcatcgacc agtagggctg ctggagatcc aggctcatgc
    29761 tccagtacag ggcggccagg gcggcggcca gactgaccac cgagaactgg acgtcccgcc
    29821 agtcgaggcg caacaggggc tcaggtcgga tcatcgtggt gggatggccc gacgtccagc
    29881 tgatggagca gggtttccag cacccggaga caggtgctca ggtccgcctc gctcacggcg
    29941 gcaaggaggc ggccgcggat cccgaccagc gcagtctcga cgcgggcggc gagtgcttca
    30001 ccggcatcag tgaggtgaag ggtgcgggcg cggcggtcct gggggtcgct gcgtcgctcc
    30061 accaggccgt gcgcgcagag ttgatcgagc agccggacca gcgaaggccc ctcgatcccg
    30121 atctcctccg ccagttcgtg ctgacgcatg ccgccgccgt ggcgggcgat atgcaggacc
    30181 gggatggcct gagcatccga gatgccatag gtcgtgagcg cccgattgac ctccttgcgg
    30241 tacaggcgcg cggcgcggat caggagggtc gtgagctggc ggcgcagggc ggaaggtgga
    30301 gtcgtcatcg tcgggcggga ctggcagagg gaagggcagg accggagcag ggacccatgg
    30361 cagtaagcgt ttaaataata gatagctatc tatctatttg caagcgggcg acgcaccacg
    30421 ccgctgacga cgggttcatc cgacctgcgg gccatcgccc cgcgacgcca gcggaccgcc
    30481 cgcagaatgg cggtttcgcg acacgctggg cgcgtcccca ggcgtcccca ggcgtcccca
    30541 ggcgtcccct cgtgcggccc cgcgcgaccc tgcgtggggc gcaggcccgg cctcggcgga
    30601 ggggcgagac ggcgctccgt tccgtgttta tggtgcgttc gatgggtcgg cacctttatt
    30661 tccgcatgcc ccacttgaaa tggcgccatg ccgcccctat tattagcact cgacgggggt
    30721 gagtgctaac agccggctcc cggtagcgcc agcaccgagg ctggccacga attgtggtcg
    30781 ggcaggtttg cccggtttga atcgagtagg agacctctac atgaaaatcc gtcccttgca
    30841 tgatcgcgtc atcgtgaagc gtatcgaagc cgaacgcacc acctccagcg gcatcgtcat
    30901 ccccgactcc gccggtgaaa agcccgatca gggcgaagtg ctggccgtcg gcaacggcaa
    30961 ggttctcgac aacggcgagt tccgcaagct ggaagtgaag gttggcgacc gcgtcctgtt
    31021 cggcaagtac gccggccaga ccgtcaaggt cgatggccag gaactgctgg tcatgcgcga
    31081 agaagacatc atgggtgtgg tcgaggcctg agcctcccgc gttccgtctt ccccgtcccc
    31141 cagaattcag gagtaatcaa gaatggcagc caaagaagtc aagttcggtg attccgcccg
    31201 cgcccgcatg gtcgaaggcg tcaacatcct ggccgacgcg gtcaaggtga ccctcggccc
    31261 caagggccgc aacgtggtgc tggagcgttc cttcggcgcc cccaccgtga ccaaggacgg
    31321 tgtgtccgtg gccaaggaaa tcgagctgaa ggacaagttc gccaacatgg gcgcccagat
    31381 ggtcaaggaa gtcgcttcca agacctccga catcgccggt gacggcacca ccaccgccac
    31441 cgtgctggcc cagtccatcg tccgcgaagg catgaagttc gtcgccgccg gcatgaaccc
    31501 gatggacctc aagcgcggca tcgacaaggc cgtcgccgcc actctggaag agctgaagaa
    31561 gctctccaag ccctgctcca ccaccaagga aatcgcccag gtcggctcca tctccgccaa
    31621 ctccgacgcg tccatcggtg accgcatcgc cgaagccatg gaaaaggtcg gcaaggaagg
    31681 cgtgatcacc gtcgaagacg gcaagtccct ggccgacgag ctcgacgtgg tggaaggcat
    31741 gcagttcgac cgcggctacc tgtcccccta cttcatcaac aacccggaca agcaggtcgc
    31801 catcctggac gcccccttcg tcctgctgtt cgacaagaag atctccaaca tccgcgatct
    31861 gctgccggtg ctggaacagg tcgccaaggc cggccgcccg ctgctgatca tcgccgaaga
    31921 cgtggacggc gaagcgctgg ccaccctggt ggtgaacaac atccgcggca ttctcaagac
    31981 ctgtgccgtc aaggcgccgg gcttcggtga ccgccgcaag gccatgctgg aagacatcgc
    32041 tatcctgacc ggtggccagg tgatctccga agaagtcggc ctgaacctcg aaaaggccac
    32101 cctgcaggac ctgggccagg ccaagcgcat cgaagtgggc aaggaaaaca ccaccatcat
    32161 cgacggtgcc ggcaacgccg agcgcatcga agcccgcgtc aagcagatcc gcgtgcagat
    32221 cgaggaagcc acctccgact acgaccgcga gaagctgcag gagcgcgtcg ccaagctggc
    32281 cggcggcgtg gccgtgatca aggtcggtgc cgcgaccgaa gtcgaaatga aggaaaagaa
    32341 ggcccgtgtc gaagacgcgc tgcacgccac ccgtgccgcc gttgaagaag gcatcgtccc
    32401 cggcggcggc gtcgccctgc tgcgtgcccg tgccagcctg gccggcctca agggtgacaa
    32461 ccacgaccag gatgccggca tcaagatcgt gctgcgcgcc atggaacagc ccctgcgcga
    32521 gatcgtcggc aatgccggtg acgagccctc cgtggtggtc aacaaggttg tcgaaggttc
    32581 cggcaacttc ggctacaacg ccgcctccgg cgagtacggc gacatggtcg agatgggcgt
    32641 gctggatccc accaaggtga cccgcaccgc gctgcagaac gccgcctccg tcgccggcct
    32701 gatgctcacc accgactgca tggtggccga gctggccgaa gacaagcccg ccgccggcat
    32761 gcccggcatg ggcggcatgg gtggcatggg cggcatggac atggggatgt aatcccgtcc
    32821 ggcccgcttc aggccctgag aaaaaccccg cttcggcggg gtttttcttt tggctgcgcg
    32881 gatttcgcgc tgacgcttga ggtgttctct caaactgacg cccacgtaag ttcacagtaa
    32941 gggatggtcg gtaccttgcc cctaccctta aaggagtctt atcgggtcct aacaaggttt
    33001 tatagaggag gagagagatg aaagacgacc tcggcgaccg catccgggca aatcccaagt
    33061 accaggagct ggtttccaaa cgcaattcgt acggctggat catgacgatc ctgatgctgg
    33121 ccgtgtacta cggctacatc ctgcttgtgg ccttcaacaa ggaattcctg gcccagaagc
    33181 tgggcgctgg cgtcatgtcc ctcgggattc ccatcggcgt gggcgtgatc ctgttcacga
    33241 tcctgatcac cggcatctac atccgccggg ccaacaccga attcgacgac ctgaaggctc
    33301 aggtcatcaa ggaggccacc aagtgatgcg cacttccaaa ctgatcctcg gcctgctggc
    33361 cgtgttgggt tccggcgctg ctctggcggc cggtgccgac ctcggccagg ccgaaaagca
    33421 ggcgaccaac tggaccgcca tcatcatgtt cggcgtgttc gtcgccttca cgatgttcat
    33481 caccaagtgg gccgccggca agaccaagtc cgcggccgac ttttacaccg ccggcggtgg
    33541 catctccggc ttccagaacg gcctcgcgat cgccggcgac tacatgtccg ccgcgtcctt
    33601 cctgggtatt tccgccgcag tgatggccaa cggttacgat ggcctgatct acgccatcgg
    33661 cttcctggtt ggctggccga tcatcacctt cctgatggcc gaacgcctgc gcaacctggg
    33721 caagttcacc ttcgccgacg tggccggcta ccgcttccag cagaccccga tccgcgcctt
    33781 tgcggcgtcc ggcacgctgg tcgtcgtggc cttctacctc atcgcccaga tggtcggtgc
    33841 cggtcagctc atcaagctgc tgttcggtct cgagtactgg gtcgcggtgg tggtcgtcgg
    33901 cgcgctgatg atggtctacg tgctcttcgg tggcatgacc gccaccacct gggtgcagat
    33961 catcaaggcc tgcctgctgc tcgccggtgc ttccttcatg gccttcatgg tgatgtccaa
    34021 gttcggcttc agccccgaag cgatgttcgc caagtcggtc gagatcaaga ctgcgctggc
    34081 cgccaaggat gccaagctgc tcgaggaagc cgccaaggtc gccgccgaga agggcaagga
    34141 agtcaccgaa gtggtcgccg ccatggccgc caagaagggt gattccatca tgggtccggg
    34201 ttccttcgtg aaggatccga tctccgcgat ctccttcggc atggccctga tgttcggtac
    34261 cgccggtctg ccccacatcc tgatgcgctt cttcaccgtg ccggatgcca aggaagcacg
    34321 caagtccgtg ggttgggcca ccgtctggat cggctacttc tacatcctga ccttcatcat
    34381 cggcttcggc gccatctgct tcgtgctgac cgacccgagc ttcctggatg ccaagggcgc
    34441 cctcaagggc ggcggcaaca tggcggccat ccacctcgcc aacgcggtgg gcggcaacgt
    34501 gttcctgggc ttcatctccg ccgtggcctt cgccacaatc ctggcggtgg tggccggcct
    34561 gaccctgtcc ggcgcctcgg ccgtgtccca cgacctctac gccaccgtgt tcaagaaggg
    34621 caatgccgat tccgcggctg aactgcgcgt gtcccgcatc accaccatcg tgctgggcat
    34681 cgttgcggtg atcctgggga tcgccttcga gaagcagaac atcgccttca tggtgtccct
    34741 ggccttcgcc atcgcggcgt ccgccaactt cccggtgctc ttcatgagcg tgctgtggaa
    34801 ggactgcacc accaagggcg cggtgatcgg tggcttcctg ggtctgatca gcgcggtcgg
    34861 aatgaccgtc ctgtccgact cggtgtgggt ggcggtgctg ggcaacccga agggctccgc
    34921 gcctttcccc tacacctccc cggccctgtt ctccatgagc atcggcttcg tgggcatctg
    34981 gatcttctcg ctgatggacc gttcgccggc cgcccagcag gagcgcgaag acttcgccgc
    35041 ccagctgatc cgttccgaaa ccggtatcgg tgccgcctcg gcttccagcc actgagcgac
    35101 atcgccggag cggtcacccg accgctccgg accggacgca agacccggcg tgccgaaagg
    35161 ccgccgggtt ttttccattt tcgacggggg tgcgccgcga cggcaccaaa tcgatggtcc
    35221 gggccctgtg ctagactcgg cgggtttcgg aatggacgcg tgccgacaac ccacgcagct
    35281 gcgcggggcg gaatcgatgc ggaacgggtg cgccgggctg tgtgcgtgca gtacgcccga
    35341 tggggcgtgc aggccggcaa aaaagacggc gggggaaggt cccgtcgggc gggctcaatc
    35401 ccccgaggcc tcgtctgcag atgaaccgca gactcagccc ccaagaccga gtaaaaggag
    35461 aacagcgagg atgactatga cagtccccgc ttacttcaaa cttacacctc catccttgta
    35521 aggtgagcga tgcgcatcct gctggcggaa gatgatctca tcatcgcgga cgggctctgt
    35581 cgcgccctca agaaggccgg ttacgcggtg gatcacgtgc ccaacggggc cgacgctgac
    35641 gctgcccttc tcgggcagac cttcgacctg ctgatcctcg acctggggct gcccaagctg
    35701 tccggcatcg aagtgctgcg ccgcctgcgg gcccgcaagt gcacgctgcc ggtattgatc
    35761 ctgacggccc aggacggcat cgaagaccgg gtgcgcggcc tggatgccgg tgccgacgac
    35821 tacctcacca aacccttcgc gctgcccgaa ctggaggcgc gcgtgcgggc cctcacgcgc
    35881 cgcggtaccg gccaggcgcg ctgtatcgag tacgcccggc tggcctacga tcaggccgac
    35941 cgcgtcgtca agatcgacgg tcaggtggtg gatctctccg cccgcgagat cggcctgctc
    36001 gaagtcctgc tcctgcgcgc cggccgcctg gtgagcaagg agcagctcgt ggaccacctg
    36061 tgcggctggg gcgaagaggt ctccaacaac gccatcgagg tgtacgtcca ccgcctgcgc
    36121 aagaagctgg agcaaagcgg cgtgcagatc gtcaccgtgc gcgggttggg ctactgcctg
    36181 gaaaagccgg atgtggtcgc ctaaatggcg cagggtggcg gatgacggcg ccgccgacgg
    36241 caagggcttc aactccctgt tcggggaaat cctcgactgg atgctggcgc cgctgctgtt
    36301 cctgtggccc atctccatca tcgtcaccca caacgtcgcc aacagcatcg ccaaccagcc
    36361 ctacgatcag gccctggccg tcaacgtgcg cgccatcgcc cgcctggtgc gggtcgaggg
    36421 cagtcaggca cgcgtgaatt tccctgcgcc ggcccgcctc atcctgcgcg ccgacgagga
    36481 cgacaccgtg tactaccaag tgctcggttt caagggcgaa ctggtcgccg gcgacaagga
    36541 catcccgacg ctgcagacgc ccgacagcgt ggtgcccgat gtggtcctgt tccgtgacga
    36601 gtccatcgcc ggagaggacg tacgggtcgc ctatcagtac ctccagcccg acgccgccgc
    36661 gccgcgccgg cttgtcctgc tgcaggtcgc cgagacccgc aacaagcgca gcaccctcgc
    36721 gtcccgcgtc gtcaccggcg tgctgctgcc ccagttcgcg atcatcccca tcgccgtcat
    36781 cctggtttgg ttgggcctgt cccgcggtat cgcgcccctc aaccagctcc agcgcctgat
    36841 ccggcggcgg cggccgacgg atctgtcgcc catctccgtc tccaacgtgc ccgaggaggt
    36901 gcgtcccctc atcatcgcct tcaacgagat gatggcccgc ctggaagaaa acctgcaggc
    36961 ccagcagcgc ttcatcgccg acgccgcgca ccagatgcgc acgcccctca ccggcctgcg
    37021 catgcagagc gacctcgccc tgatggagac tgatcctgca cagatccatc gctccctgca
    37081 gcacatctcc cagagcgccg agcgcgccag ccacctcatc aatcagctgc tgtccctggc
    37141 ccgcgccgaa gccagttacg aaaagatcta cgctgtcgaa cgggtggacc tggaggccct
    37201 gctgcgcgcc atgctcaccg acttcgtccc gcgggcacgc cagaaggcca tcgacttcgg
    37261 cctcgaaacc accggcggcc ccctcgtcat cgacggcaat ccggtgctgc tggccgaact
    37321 cctcaagaac ctcatcgaca acgccctcaa atacacgcca gaaggtggcc gcgtcaccgt
    37381 gcgcaccctc gccaccgaaa aggccatcgt cgaagtcgaa gacaccggca tcggcgtgcc
    37441 cgaagccgac cgggagcgcg tcttcgaacg cttctatcgc gtgctgggca gctccgaaag
    37501 cggctccggc ctgggcctgc ccatcgtccg cgagatcgcc gagctgcacc gtgcccacgt
    37561 cagcctcaac cccaacccgg ccggcagggg caccatcgcc caggtcgtct ttccgcgcag
    37621 ccgccccacc ccggaagaga ccgcagccca gaccacccag gccctcaccg cccccggcgc
    37681 cacccagttt ccgcccgcct gaaacctggc gtccttccgg gattttcttg acaggacgat
    37741 gaaccaaacc tatactgcgc gcttcatctc ggccaggtag ctcagttggt agagcagcgg
    37801 attgaaaatc cgcgtgtcgg cggttcgatt ccgtccctgg ccaccaaaat tcagcgccca
    37861 actgttctca gttgggcgtt ttcatttgtg cttcccgcat gacacgcggg gttcccgcac
    37921 attctgcgtg tgcgaccggg gtcctgtggt ctggcaggaa catccccggt tgccctccgt
    37981 tccgccctct cttctctcga aatcggcgct aacttcttcg ccgtggcaca cgcacatggc
    38041 ctttcgcgac catgatttgt gcgcgtgcca ttgccattcg ttgtcgggct tgcttggagc
    38101 agcagtccgt cacgccaaca ctgccgccgc gcaaggcatg gaatacaagc ctgcacgttg
    38161 ctgcgtgacg acgagtgctc cgtaggccgc cagtcgagcc tcgtgctccg gcttgccctt
    38221 gtccttcgac ttgatcttga acaggtcaat cggcttgtcg ctcttctggc cagcgcgctg
    38281 catgatcctt tcgccgttca cctcggcccc cttgaacgac cagagcgctt cgaacacctt
    38341 ggcctgacca tcggtgaacc ggatcggtgt ggcctgcaca tccggcagcg ttgcccactt
    38401 gaagtcggcc gagaacggcc cgttcactgg cgcagccgga tcggccactg ctggcactgc
    38461 agacggcacc gagggtatga aggtgattcc gcatccatag aacgtgaatc gttcttccag
    38521 cgccagccat tccacaccgg agccgaaggg cgatccacga gtcgtgcgcg gcctgggggt
    38581 gatgactcgg atgttgcttc ccgccacccg gacacgatcc agcaaggacg gctcatgcag
    38641 cactctcgtc agatctcggg ccagcactac cggagagctg cgtgcgttac cgagccgcca
    38701 aacaccgtcg cccaagtcat cgatgtcgtc gcggctctcg atgtcgagcg cgaggcgcat
    38761 tttctgccgc agccagtctt catccagggc caacgccgca ccgtcgattg cctcgacagg
    38821 aacctggcca cactcctggc accggcagat ccgaccgccc cgtccatcgc cccagacctg
    38881 aagttgcccc catcccgtgg acaggtcatg gtttgaaact caagcgctcg g
//
LOCUS       DAOQPA010000019        38224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_433_length_38224_cov_17.159868, whole genome shotgun sequence.
ACCESSION   DAOQPA010000019 DAOQPA010000000
VERSION     DAOQPA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 38224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38224
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_38224_cov_17.159868"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..854)
                     /locus_tag="PKX01_04455"
     CDS             complement(141..854)
                     /locus_tag="PKX01_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain N-acyl amino acid synthase"
                     /protein_id="HMZ75406.1"
                     /translation="MTDRACHSHYHSVEPPPRGVHLQDPRRFTLERADPVRHGQTVRM
                     LIDRMYSWRGLTPVGSHHGPHHNHHRTTLVTSSADEVFGTITVGIDSPAGLQADPLYR
                     HELDAFRQRGRLLCEITGLAVDSPVQAKEALGGLFHLAYIQARLIDKADDVVIEVNPR
                     HAPFYQRLLGFGQIGPLRHCQRVNAPAVLLHLAISHMDDHIARFGGTSPKHTRTLYPY
                     FFSLAEQAVLLAQMQQAAN"
     gene            complement(1033..2757)
                     /locus_tag="PKX01_04460"
     CDS             complement(1033..2757)
                     /locus_tag="PKX01_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMZ75407.1"
                     /translation="MLTALVQRLRPYRRRVLVAALALVLAAAAMLAVGQGLRAVIDQG
                     FAARDPAWLNRSLAATFALIALLAAATYTRFYNVSWLGERVTADLRQQLFDHLLSLPP
                     GWFEQGRTGEVMSRLTADTTQIEQVIGSSLSIALRNALLLAGGLAMLFTTSLKLTLLT
                     LAGVPLVVAPILLYGRRVRRLARESQDRVADLGNRIDETLHEIRIVQAYGHEDADRQD
                     FARLVETGFATARQRIANRARLVAAVLLLVFGAIAFILWVGGHDVLEGRLSAGELSAF
                     VFYAALVAASVGALSEVWGDLQRAAGATERIAEILAIRPEIVVPANPRPLPDPPRGAL
                     HFERVRFHYPSRPDTPALDDFSLSVTPGETVALVGPSGAGKSTVFQLLLRFYDVAAGE
                     IAVDGVPLPQADPAALRRRMALVPQEPAIFAASVLENVRYARPDADEAAVRAACRAAF
                     ADEFIDRLPEGYATWLGERGQRLSGGQRQRIAIARALLADRPILLLDEATSALDSESE
                     RMVQAALDELMRGRTTLMIAHRLSTVRRAHRIFVLDHGRVVQHGSPTELLAQGGLYAR
                     LAGQQRPE"
     gene            2830..3234
                     /locus_tag="PKX01_04465"
     CDS             2830..3234
                     /locus_tag="PKX01_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HMZ75408.1"
                     /translation="MLSTLPLTAGVTLLIVLLMFGTCFAVGKARHTYGIQAPAVSGHP
                     AFERLYRVQMNTLEWAAMTLPALWICAVYVGDGVAAALGGVWLVGRVWYALGYARDPA
                     LRARGFGIAALAFGALGLAAGGGVIRHLLGVS"
     gene            3231..4298
                     /locus_tag="PKX01_04470"
     CDS             3231..4298
                     /locus_tag="PKX01_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HMZ75409.1"
                     /translation="MRRSLRRVAVLAAIALAGPVGAAPALPALGVEGDGVTVSGISSG
                     GYMAVQFQVAFSRIVKGAGVLAAGPYECAEGSVAKALTRCMAPNRLAPPPTVADSVAR
                     VDARARAGQIDAPAGLAEDRAWVLSGGADRTVERPVVDALVAFYRHWLPATSVAYVRL
                     DDAAHGLSAGHGMISVSDAQANACATSEPPFINRCGNFDAAGELLRFLLGPLQARGDA
                     RAGRLDAFDQRPFVSGLAADAGLAEEGLVYVPDACRAGGCRLHVAFHGCRQNLDQVGR
                     RFAEGAGYAEWAATNRLVVLFPQTVTRNGWAWGSWRWVVNPKGCWDWWGYSGADYATR
                     TGPQMAAVRRMIARLAEAPGR"
     gene            complement(4356..5132)
                     /locus_tag="PKX01_04475"
     CDS             complement(4356..5132)
                     /locus_tag="PKX01_04475"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HMZ75410.1"
                     /translation="MSKLTTEKVLSVHHWNDSLFSFKTTRDTALRFENGQFVMIGLDV
                     EGRPLTRAYSIASPNYEEHLEFFSIKVPNGPLTSRLQHLRPGDPIVVSKKPTGTLVLH
                     DLLPGKHLYLFSTGTGLAPFLSVIQDPETYERFDKIVLLHGVRYVSELAYSEFIEDFL
                     PQNEYFGDAIRDKLIYYPTVTREEFRHQGRVTDLVTSGKLFEDIGLPPLDPAVDRAMI
                     CGSPHMLKDCCDMLDARGFKVSAHIGAPGDYVIERAFVEK"
     gene            5245..6171
                     /locus_tag="PKX01_04480"
     CDS             5245..6171
                     /locus_tag="PKX01_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ75411.1"
                     /translation="MRYTLRQLEIFVAVARHESVSRAADEIALSQSATSAALSEFERQ
                     FDTQVFDRIGKALRLNELGRLLLPEAVALLEHGRAIEGLLMRQEAFGPLQIGATLTVG
                     NYLATLIAAEFLRRHPQVRIGLQVHNSATIVQQLLRFELDLGLIEGSCRDPELIVEPW
                     VADELVVFCAPTHPLAAAGQASVDALLRQPWILREQGSGTRETFEQAMRHVTGAFDVR
                     LELEHTEAIKRAVESGLGIGCISRLALREAFRRGSLVPIEVPELDLRRQFQFVTHARK
                     YVTAGMGEFIALCRQLSAGATRSDEIELPFIP"
     gene            complement(6161..6637)
                     /locus_tag="PKX01_04485"
     CDS             complement(6161..6637)
                     /locus_tag="PKX01_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ75412.1"
                     /translation="MNLLLTARDVYRVVVAQLQRLSPLFDLAIRLYVARVFILSGLVK
                     LQSWDSTLALFENEYEVPLLPPEIAAYMGTGAELILPVFLALGLGGRFFAFALFVFNA
                     VAVLSYPDLTPAGLKDHILWGSLLLVTLFHGPGPLAADRLLGTESPVGPTPAPVTE"
     gene            complement(6634..7410)
                     /locus_tag="PKX01_04490"
     CDS             complement(6634..7410)
                     /locus_tag="PKX01_04490"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HMZ75413.1"
                     /translation="MSLHELQQAFSAALLHGASADHLIAGPRGLGAADAAAIYRDALF
                     ANYRRALAASFPVVERLIGPDCFRQTADALVRAVPSTDGDVAAFGAHLPDFLADHPPT
                     RGLAWLPDVARLEWAIEMAAGAPDATPFDPAALAVDAPEALRLALHPSAWLLASHHPT
                     LAIWRANQPDRDGTVDAAAAAGGACLLVVRRPDDVHVEALTPAEFAWLAALAAGQPLG
                     DAVTAAFDTDPAFDLPATLTHHVRAGTLAGSSSAPERKLP"
     gene            complement(7397..8242)
                     /locus_tag="PKX01_04495"
     CDS             complement(7397..8242)
                     /locus_tag="PKX01_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HMZ75414.1"
                     /translation="MPSSAPLRSPRALAGLGLRTPHIHEILATRPPVGWLEVHSENYF
                     APGGSGIGLLERLRSHYPVSLHGVGLGLGSADPLDDTHLDRLEALIDRIDPFLVSEHL
                     CWARVAGRHLNDLLPLPYTHEALDLVCARIDAVQTRLRRRLLVENVSSYCRFHADALN
                     EWDFVAAVARRTGCALLVDVNNIYVSSRNHGFDPLAYLDALPADAVAELHLAGHEAAH
                     GCLIDTHSRPVCDAVWALYADALERFGPRPTLLERDQDIPPLAELLAEVYRAADCLEA
                     CHVPA"
     gene            complement(8309..8599)
                     /locus_tag="PKX01_04500"
     CDS             complement(8309..8599)
                     /locus_tag="PKX01_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005475027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HMZ75415.1"
                     /translation="MTTITRTAPATQTVRAAVAGLLALGLAAGAMPAHAAKGDNEKCA
                     GVVKAGKNDCATASSACAGTVSKDNQKDAWIYVPKGTCEKIAGGVVVADAKK"
     gene            8818..9075
                     /locus_tag="PKX01_04505"
     CDS             8818..9075
                     /locus_tag="PKX01_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75416.1"
                     /translation="MTSRTSGIALATAAAALFATAMAAPVAQAADAPFVKCTGINSCK
                     GTSECKTAKNECKGHNSCKGMGWVKKASADECTSAGGKVMK"
     gene            9196..10038
                     /locus_tag="PKX01_04510"
     CDS             9196..10038
                     /locus_tag="PKX01_04510"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HMZ75417.1"
                     /translation="MSTASTIVAGFGLGLRTDHYRDFTEGRPPVDWLEILSENYMVPG
                     GKPLHFLDRIRRDYPMVMHGVSLSIGSADPLDRAYLADLKTLVERVEPAWVSDHLCWT
                     GVDHAHLHDLLPLPYTEAALRHVTERVQAVQDVLGRRLVLENVSSYVAWQADEMSEWD
                     FLAEVARRADCEILLDVNNVYVSARNHGFDPRHFIDAMPAARVRQIHLAGHEDLGDVV
                     IDTHDHPVCDDVWALYAYTVRRLGLVPTMIERDDRIPPLQTLLAELDLARRTAQGALQ
                     EAVA"
     gene            10035..10829
                     /locus_tag="PKX01_04515"
     CDS             10035..10829
                     /locus_tag="PKX01_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HMZ75418.1"
                     /translation="MTPLANQQRALQTAILSDVAPAGLLAGPDAARRFSIYADAYRAR
                     LAAALRDNYPVLHRVLGDEDFDALARAYLHAHPSVAPSIRWFGGALADFVQATPDALP
                     HPALVDLIRLEWAMRAAFDAEDAPPMGFATLAALPPDRWPALRLRLHPSVSRLALEWA
                     VEPLWQALAPLEDGQSPEQEPPPPEPHVHGLIAWRQGLTPCWRSVEALEGHLLAALAG
                     GAAFADLCTLAADAGEEAAAHQVAGLLRRWVDEGLLQACDALMSTP"
     gene            complement(11248..11949)
                     /locus_tag="PKX01_04520"
     CDS             complement(11248..11949)
                     /locus_tag="PKX01_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HMZ75419.1"
                     /translation="MIVKALIFDVFGTLVDWRTSVAREVTAHLAPQGVQVDALAFADA
                     WRDQYQPAMEAVRSGRLPFCKLDRLHRHNLDVVLADFGLDAVDESTRQSLNLAWHRLD
                     AWPDVAPGLHRLRRHYRLAPCSNGNISLMVDLARRNDFPWDAILGAEVARDYKPKPAV
                     YLAAADAFDLTPAETLMVAAHSSDLDAAARAGLRTAFIARPDEFAQGAGERMPTVPVD
                     LTAVSLTDLADQLTP"
     gene            complement(11946..12809)
                     /locus_tag="PKX01_04525"
     CDS             complement(11946..12809)
                     /locus_tag="PKX01_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ75420.1"
                     /translation="MELIALRTFEAVVEEGGILAASRKLNTVQSNVTGRIRRLEEELG
                     TELFFRKGRGLELAPPGRVLLDYARRMLQLERETSAALRQVGESVGELRIGAMETFTA
                     LHLPAALKSVRRHHPAVELRIETAPSAVLVERVLDHKLDCAMVAGPVDHPALDFCPLV
                     DEELVQICAIGTRPVHLPLILFREGCAYRTRALAWQRAMGHALSDAMEFGTVEGILGC
                     VAVGLGWTLMPRRVVAQSVHAPDLVVESVPEDIAHVPTGMIRLRGAPPMAAVDTLVAA
                     FQPIAPGGPRP"
     gene            12906..14102
                     /locus_tag="PKX01_04530"
     CDS             12906..14102
                     /locus_tag="PKX01_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HMZ75421.1"
                     /translation="MSSRRERFQVMGAGICSLILVLGVARFAYTPLLPLMQRQAGLGV
                     GEAGLLAAINYGGYLSGALIASLISDLVLKDRLYRIGMVLAIASTALMGLTTDVTVWA
                     VSRFVAGLSSAAGMLLGTGLILNWLIRHDHRSELGIHFSGIGLGIAGCAAAVLLMSPW
                     FDWRGQWLIFALLGGMLLVPALRWLPAPDPSPFTKSGEALRDQPPGRLFMRLFMAAYF
                     CAGVGYVVSATFIVAIVDRLPGLAGQGALVFLAIGAGAAPACIVWDFVARRTGALNAL
                     ILAAVLQIAGILLPVAVGGSLAAFVGALLFGGTFIGMVSLVLTMAGRYYPTRPAKMMG
                     KMTLSYGAAQILGPALTGWLALHWGSYTAGVYLAAGMMAVGTVLLLVLKGVERRDAAA
                     LRAVAA"
     gene            14157..15938
                     /locus_tag="PKX01_04535"
     CDS             14157..15938
                     /locus_tag="PKX01_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ75422.1"
                     /translation="MPAPVEYTIRAAHPAAHLFEVTLTLAEPDVAGQVFSLPAWIPGS
                     YMIRDFARHIVSLQAEAGGRAVAAVKDDKHTWRLPAGLRGPLTLRYTVYAWDLSVRTA
                     HLDETHGFFNGTSVFLRAHGFEDRPQRVRLLPPAGLEAAGWRVATSLPEASPEVGGAE
                     RYGFGVYEADTYDALIDHPVEMGCFDLFEFEACGVPHAVAITGRHDCDGERLVADLKP
                     VCEWQIRLFGEPAPMERYVFLVMAVGDGYGGLEHRASTALLCSRNDLPHRGMGAPTEG
                     YRQFLGLCSHEYFHTWNVKRIKPAAFVPYELGRENYTRLLWAFEGFTSYYDDLALLKS
                     GRISRREYLDLLAKTVSAVHKGSGRLKQSVAESSFEAWTKYYRQDENSPNAIVSYYTK
                     GALVALLLDLRLRGESGGACSLDSLMGVLWRDHGQTGVGVAEEGLYAAAAALGGASLG
                     RWLRNLVEGTDELPLEDGLRRVGVKLAWEASVKCPGMGARTTVEGESVKLANVFDGGA
                     AQAAGLSAGDVLVALDGLKVTGKTLDALLARRMAGETVQVHAFRRDELKTFSVILQAP
                     LPDTARLTEAPRCSAAARALREGWLGA"
     gene            complement(15986..17020)
                     /locus_tag="PKX01_04540"
     CDS             complement(15986..17020)
                     /locus_tag="PKX01_04540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ75423.1"
                     /translation="MPFIDLLKACASQLIVLHHLVIYGPLSEAMADVMPALVAWIAEY
                     GRLAVPVFLAAAGFLSARGLAPGGVPTRVQPLTLIARRYMRLAIPYLVAVTCALACAA
                     VARTWMHDDATPAIPTIRQFLAHVFLLHSLLGYDSLSAGVWYVAVDFQLYALMVGLVW
                     LAHSGRGAASPTQRLMMLVAAAAAASLLYFNLDQDWDSWGLYFFGSYALGAFAYWAAG
                     RSSHGWLVLLAAAGTLVLVVDFRVRIAVALIASLSMALACRHALFQRISPTGIVVFLS
                     KISYSTFLIHYPVSLVFNTLFANVAGHQPALAVTTFVLTWSTSIGAGTLLHRYVEDGP
                     VFAVRRPVPA"
     gene            complement(17102..18538)
                     /locus_tag="PKX01_04545"
     CDS             complement(17102..18538)
                     /locus_tag="PKX01_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ75424.1"
                     /translation="MPTPQEPAAPRIPSAEPSLARRILQLAWPVLIAQLSSMTQMVSD
                     TVLTGRYGTEDLAAVAVGSGIYIAIAMLMVGILQAVAPTVAHHVGAGRHEAIGPDLQQ
                     GFWLALLLTVPAVLFLRHPDCLMQLSHMSPAVEAKSRDYLSTIAWGLPAVLLYRTFYA
                     FANALGRTRVLMLISLAATSVHIPLSWLLINGHAGFAPLGGTGCAASSATIAWFALGC
                     AMLYLLRNPAYARYRLFTRWQAPRPAALAELLRLGLPMGFSSFVEITAFTLIALFVAE
                     LGAAVVAGHRIVANLSALIYMLPLALATATLVLVGQSAGARDDVRARQAAWCGILLAT
                     GLSTTVSVLVWLVRDRVVAAYTTDPAVGTLADALILYICLYQFFDAIQTVAAHALRGY
                     KITFGPMLVHTLCFWGIGLAGGYLLAFRGIGPFPPQGVAGFWQASVLSNIAAATLFGT
                     YLYRIARLKRPAEDAAVRQKIPYRTEKS"
     gene            complement(18573..20009)
                     /locus_tag="PKX01_04550"
     CDS             complement(18573..20009)
                     /locus_tag="PKX01_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HMZ75425.1"
                     /translation="MLWCALYLPDLALQVFTRGHADATPLALLTPRPRVRVVAANPAA
                     MACGVAPGISRASALALAPGLTFAERDPDLEQAALEEIATWAGRFAGTLSLDPPGAVL
                     LEVRGSLRLFGGLPRLAEALLDGLAELGYTGRLGAAATPLAARWQARQGLSPGVGSLT
                     DDQATLDALPLTVLDDASGPGADVLELLAGLGLQRLGALRHLSRHGLARRHAQALTDC
                     LDRAYGRTPDPRPSFVPPERYSARLPLPAPTDQVDMLLFGLRRLLAGLSGWLSARQAG
                     VERFQLVLEYAGTGVETLPVILGKLANDPSRCQLLAREHLTRLLLTAPVEALRLETEA
                     PRPLAPSATDLFDAPSGPRSDPALLLATLRARLGDEAVFTCAPHADHRPEQAWRRGTP
                     RREHAPADVHTPAGPRPLWLLPRPRAVTAPAPERLLAGPERIESGWWDGAGVRRDYYV
                     ARRSDDSLVWLFQDLEPPYGWYLHGYFA"
     gene            complement(19945..20661)
                     /gene="imuA"
                     /locus_tag="PKX01_04555"
     CDS             complement(19945..20661)
                     /gene="imuA"
                     /locus_tag="PKX01_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HMZ75426.1"
                     /translation="MSASARLHAVDSLPGHLVWRGDRLAVPAEAGIPSGFPDLDACLP
                     GGGWPNAALSELLIPRPGIGELGLLLPLLARTAEDHWSVCIAPPLHPYAPALAAGGVP
                     LGRILFVQTESPAHARWAARQALASRSCTAVLMWLAGTDMAALRRLQLASEDSATPLF
                     LIRPEHCATQASPAVLRLALRPRVGGVEIRILKRRGPLFAGTLALELPGPVPPRPRPH
                     ALVRPLPARSGPPGLHAGTC"
     gene            complement(20838..21440)
                     /gene="lexA"
                     /locus_tag="PKX01_04560"
     CDS             complement(20838..21440)
                     /gene="lexA"
                     /locus_tag="PKX01_04560"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HMZ75427.1"
                     /translation="MSEKLTARQQEILQLIRDTVEHEGRPPTRAEICTAFGFRSPNAA
                     ETHLRALAAKGVISLEEGRARGIRLTEALGLPLVGRVAAGSPILAAEHVQGRFQVDPS
                     LFSPRADYLLKVRGMSMRDAGILDGDLLAVHRTAEARNGQIVVARVDDEVTVKRLSRS
                     GPIVELLPANPDFEPIVVDTRRDLLEIEGLAVGLIRSEPL"
     gene            21777..22280
                     /locus_tag="PKX01_04565"
     CDS             21777..22280
                     /locus_tag="PKX01_04565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMZ75428.1"
                     /translation="MGQPAPLFALPDADMEIFDLASALASHHVVLYFYPRDNTPGCTR
                     QAIDFSDHDGDFARERCIVVGVSPDDCLTHADFRDHHGLSVQLLSDEDYEVCKLYNVW
                     RSQEVDGVMKQGVRRSTFIISCDGVLQHVLHDVNPRGHAAEVFKLVQQLNTRKFNANR
                     QEHGRHA"
     gene            22246..22725
                     /locus_tag="PKX01_04570"
     CDS             22246..22725
                     /locus_tag="PKX01_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ75429.1"
                     /translation="MQIAKNTVVTLDYQVRDPDGNMIDDGAHPLVYLHGGYDGIFTVI
                     EEALQGKNVGEQLNVKLQPDDAFGEYDEELVLVEDASLFPDNIEVGMSFERVGEDGED
                     EQIYRITDIADGKVVVDGNHPLAGTALVFDITIKSVRAATAEEIGHGHVHGEGGHHH"
     gene            22840..24849
                     /locus_tag="PKX01_04575"
     CDS             22840..24849
                     /locus_tag="PKX01_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75430.1"
                     /translation="MKGQSLLQVDGLRVELRAGGRRVYPVDGVHVTVPAGQTLALVGE
                     SGCGKSMTALACMGLLPRGGRITAGQVKLAGEALERLPEVRMRDVRGRRMAMIFQEPA
                     SSLNPVMTVGDQIGEVLVRHRSLHGQPAWNEAERLLAAVGIPDPRRRLGEYPFQLSGG
                     MKQRVMIAIALAGEPELLIADEPTTALDVTIQAQVLDLLRTLQARRGMGMLLITHDLG
                     VVARMAHRVAVMYAGEVVEAGEREAFFAAPLHPYSRKLFAALPDAGRRDHALETIPGQ
                     VPPLGARLRGCRFAERCPQVRPRCRDAQPALHEVDGRQVRCHLYDAESGLSWLSAEAV
                     PAGRAATSPDPRPVLEVDGLKVHFPVRKGVFRRVTGQIKAVDGVTLSVAAGETVALVG
                     ESGCGKTTAGKALLQLVPASGGAVRYDGRELLTLAPADWRPVRRELQMIFQDPFSSLN
                     PRMRVGEILEEGMRSLGVEPDAAQRALRIEALLQRVGLDPSMRDRFPHEFSGGQRQRI
                     AIARALAVSPRLIVCDEPTSALDVSVQAQILNLLKDLQGEFGLSYLFITHNLAVVDYL
                     AHQVAVMYLGRIVERGRAEEVLRDARHPYTRMLLAAVPKVSGPVLTAEQGAGEAIDLP
                     SPLSPPAGCHFHPRCSEATVACREIYPDETRLSPTRVVRCHLYAS"
     gene            complement(24952..26385)
                     /locus_tag="PKX01_04580"
     CDS             complement(24952..26385)
                     /locus_tag="PKX01_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ75431.1"
                     /translation="MFQRVIALWVLLVGVGPALADGVPDVPLQLAQATSLSDAVGLQA
                     PKAFEVREQHVKVPTVDLTIEPNDLWDRIRRGFSMPDLNTDLVTDRQIYYINRPGALR
                     QMFSRGQRYLFHIVEELERRGMPTELALLPMVESAFNPTALSPAQASGLWQFIPSTGK
                     NYKLEQNWWVDERRDVVASTSAALDYLQTIYEMHGDWQLALASYNWGEGAVGRAIAKN
                     RAAGLPTEYQYLNMPGETRYYVPKLQALKNIIAQPELFGITLPPIANRPYFVTVESRV
                     SLDLATAARLAETPVDEILALNPGYKRPVLPKNGTQSLVIPAEKLEIFLNNLETHDPS
                     TATWRTYELQEGEGLEGVADRFGISSSRLRQVNGLSPRARIGSGYTLIVPSEAPGERI
                     ALANMLPTHPQAGAVEPPPAGRTLIQRTVVRGHNGRPMVVERRVVVTPPPAGAHRGAP
                     APGRAAAPATKSAPPAKAAPAAPHKRR"
     gene            26603..27556
                     /locus_tag="PKX01_04585"
     CDS             26603..27556
                     /locus_tag="PKX01_04585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ75432.1"
                     /translation="MRALLIEDTLTSATLVSHQLRNIGIEPILARDGERGIELFKQER
                     PDLVLLDVILPGMDGFEVARRIRQLEQAGEWTPIIFLTARTGDEDLEKGIAVGGDDYL
                     IKPVSETVLAAKVRAMQRIAQMRYSLLVLTRKLDEANQELTRLSSLDGLTAIANRRQF
                     DETLQREWRRASRQGKPVSLLFCDVDFFKQFNDGYGHQVGDECLKAVARTLKDVLRRP
                     ADMVARYGGEEFAAVLPDTDIAGALQVAEALRAAVEALGITHRFSRATKVVTISVGAA
                     TAVPARQDGSPMPLLKMADEALYAAKENGRNRVATVAPRAV"
     gene            complement(27578..28342)
                     /gene="gloB"
                     /locus_tag="PKX01_04590"
     CDS             complement(27578..28342)
                     /gene="gloB"
                     /locus_tag="PKX01_04590"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HMZ75433.1"
                     /translation="MDIIPLHAFSDNFIWLLRAGRYAVVVDPGDAAPVQACLAAEGLQ
                     LAAILTTHHHDDHAGGVAALTAAAPVPVYGPRDGLPVAGLTHPVGEGDVVTIPEINAT
                     FEVLHVPGHTAGHLAYYAAPALFCGDTLFASGCGRVFDGTLAQLFASLARIAELPDDT
                     QVYCAHEYTLSNLKFAAAVEPDNPAIAARVAECQALRARDLPTVPFRLGGERLVNPFL
                     RSREPAVIAAARAHGLEGDATPAAVFAAIRGWKNRF"
     gene            28377..29150
                     /locus_tag="PKX01_04595"
     CDS             28377..29150
                     /locus_tag="PKX01_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ75434.1"
                     /translation="MSPAESFPGLHAWLETPLGRYVMDWEADRHDRVVADIFGYNAVQ
                     LGIPEFDFLRANRMPFRCKAGVGDVRVQADYGALPFAANSLDLVVLPHVLEFAAHPHQ
                     VLREVERVLVADGQVVITGFNPYSLFGLRRLAAGECMGYPWHGQYLSQHRLKDWLALL
                     GFEMRLCDAGCYIPGVEQEQWIERLRLLDRAGRRWWPFMGGVYMVQAIKRVQGMRLIM
                     PRWDRKAGARALAPLVQKSTAPRAGCAQQNRNDVYFGRD"
     gene            29137..29601
                     /gene="rnhA"
                     /locus_tag="PKX01_04600"
     CDS             29137..29601
                     /gene="rnhA"
                     /locus_tag="PKX01_04600"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HMZ75435.1"
                     /translation="MDEIDIYTDGACSGNPGPGGWGAILRSGAHEKELWGGEPATTNN
                     RMELLAVIRALEALKRPVAARVHTDSQYVQKGISEWIHGWKARGWKTASKAPVKNEDL
                     WRALDAQAARHQVKWLWVKGHAGHVDNERADALARRGVEAVRKAGAAVEGAA"
     gene            29598..30320
                     /gene="dnaQ"
                     /locus_tag="PKX01_04605"
     CDS             29598..30320
                     /gene="dnaQ"
                     /locus_tag="PKX01_04605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HMZ75436.1"
                     /translation="MRQIVLDTETTGLDWKGGDRVIEIGCVEIVDRKLTGRHYHVFIN
                     PERDIDAGAIAVHGITNEFLVDKPLFRQIVEEFEAFVAGGELVIHNASFDVGFLNNEL
                     GLLGRPALTAICPSVIDTLKLAKEQNPGKKASLNALCTLYEIDNTHRSLHGALLDAEL
                     LADVYLAMTRGQESLIMALDEPPPRLDDGSGGAAASVWPPLRVILASAAEQAAHDAVL
                     AEIAKANKGHCLWIPPAPPAES"
     gene            complement(30322..31422)
                     /locus_tag="PKX01_04610"
     CDS             complement(30322..31422)
                     /locus_tag="PKX01_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ75437.1"
                     /translation="METVDCVVVGAGVVGLACARRLAQAGREVLILESEARFGSGISS
                     RSSEVIHAGLYYPPGSLRARLCVPGRDALYTYCAARAIPHRRLGKLLVATDDAQLPQL
                     DAIAACAIANGVQGLRRLSATEACALEPALTCTGALLSPDTGIVDSHALMLALLADAE
                     AAGTTLVTHVPVLGGRCSDAGIELYTGGTAPMTLRARSVINAAGLGAIRLAGALEGFP
                     ARCVPVARYAKGNYFALQGRSPFRHLIYPIPEAAGLGVHLTLDLGGQARFGPDVEWIA
                     APDYRVDSDRAAAFYPAVRRYWPALPDGALVPAHAGVRPKIHGPNEPAADFRLLGPAE
                     HGIAGLVQLFGIESPGLTCCLALADAVAAALG"
     gene            complement(31422..32174)
                     /locus_tag="PKX01_04615"
     CDS             complement(31422..32174)
                     /locus_tag="PKX01_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HMZ75438.1"
                     /translation="MSVPIDISEEAGVRFLHFGSEWVQGAMRLRKPWSLELVYTREMM
                     AGLLLHPRPWPRKVLLIGLGAGSLARFVHHHLPDAVCTVVEIAPEVHAVARQFFRLPD
                     ENARFRVVIGDGAQYVMDTDAHWDLIAVDGFDRHARAGDLATLPFYQACWTRLSERGV
                     MAVNLFSGQRGFKGRQDAIREAFDGRLCMLPPSETGNAIALAVRGAPIELSHDALLAR
                     AAALKADTGLDLHPTLKRLDAAGGLPDGRLSV"
     gene            32258..32560
                     /locus_tag="PKX01_04620"
     CDS             32258..32560
                     /locus_tag="PKX01_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75439.1"
                     /translation="MVSFENYDSELESIDMELARLAQLCGIDLRRPGVAEQVLKGDAS
                     LCTTSNPIAWEKLRGLLVLHYHVVQEEASEEGNAVAADSVRRAVERVTARMKSGRQ"
     gene            complement(32570..33529)
                     /locus_tag="PKX01_04625"
     CDS             complement(32570..33529)
                     /locus_tag="PKX01_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75440.1"
                     /translation="MKTLLASALAGLALAAAPVVDAADGFVFGAFGDTPYNRFERLHL
                     PNLLAEMDAEPLEFVIHDGDIKNGGERCDDVLFDDRLALFQTSRNALIYVPGDNEWTD
                     CHRASNGRFDPLERLRKLRGMFFPKAGHRALGQTALALETQADSADFPEYVENQRWQI
                     GPVVFVAVNVTGSANNYGYGDAPSEEFLKRDAAVQAWLAESFARARANGAQGVVIAMQ
                     ANPDIEDFGAGRHPRGFDRLMKQLLAETRAFAGEVLLIHGDTHLHRIDQPLKDPASGE
                     TIRNFTRLETYGSPFMGWVKVAVAPGERPLFRFQSRPYTPRRD"
     gene            complement(33526..36102)
                     /gene="mutS"
                     /locus_tag="PKX01_04630"
     CDS             complement(33526..36102)
                     /gene="mutS"
                     /locus_tag="PKX01_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ75441.1"
                     /translation="MKHDTPAPDISAHTPMMQQYLRIKQAYPDTLLFYRMGDFYELFF
                     EDAEKAARLMDITLTTRGASAGQPIKMAGVPHHAVEQYLARLVKLGESVVIAEQVGDP
                     GATKGPMERAVSRIVTPGTLTDAALLDDKADAPLLALTLHRGVLGLAWLNLANGDFRV
                     LECDAPQLAAQFERLRPAEVLIPDGLRLPLLESLAPSLRRLADWQFDAETARRVLAQH
                     FGTRDLAGFGAEEMPVALAAAAALFEYARATQRQALAHITALTAERDSAYLRLDAATR
                     RNLELTETLRGEAAPTLLSLLDSCATSMGSRWLRHALHHPLCDRDAAAHRHAAVGELL
                     DGAAESARRVLRGVADVERITARVALKSARPRDLSSLRDSLARLPELRAALGLPQAPL
                     LAQMLDALATPEAALDLLTRAIAEEPASVLRDGGVIAAGYDADLDELRGIQSNCGAFL
                     LELEARERARTGIPNLKVEFNRVHGFYIEVSAANAAKGELPDDYRRRQTLKNAERFIT
                     PELKAFEDKALSAQERALAREKLLYEAVLDSLAEAIPVFQRIARACALLDGLCAFAEA
                     AARYDYAMPVFSAQPGVDIRAGRHPVVERQVENFIANDCRLDATRRMLLITGPNMGGK
                     STTMRQVALIVLLAHVGCFVPAAAARIGPVDAIFTRIGASDDLASGRSTFMVEMTEAA
                     AILHGATERSLVLMDEIGRGTSTFDGVALAVAIARHLLDKNRCWTLFATHYFELTRLS
                     QDYPECANVHLDAVEHNHGIVFLHALEDGPASQSYGIEVAALAGIPGSVVRDAKRRLR
                     ALENREIGAGPQADLFAALPEPEAEAPAHPALTALAGIDPDSLTPREALEALYDLKRL
                     TR"
     gene            36335..37657
                     /locus_tag="PKX01_04635"
     CDS             36335..37657
                     /locus_tag="PKX01_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75442.1"
                     /translation="MPANSALPAITRLYQACEPQESLAPSDPRWVDFDPVRGEDNVVE
                     QCARSLRRADPAKPYFGLFSGHRGVGKTSELQRLKAQLEAGANGSKGFLVVFGDVSDA
                     LDVNDLDFPDLLVFIAAHLQKELAAKGLRGFNPITTYLTKTWDDICALLTAEVELKNV
                     RADTGFGKLTMELRNRPNSRSQLRDAVEKHSTSLLQAVNDLLDSAQAAALSAGFAGMV
                     LIIDGLDKLVRRGLGEGSNTHDRLFIDRCDQLASLKVHTIYTVPISLIYSPFFAQVEE
                     TFGKCNAPVSMIRLHSRFDEPVKPDSEGMLKMREMVDKRCSYAGIAPDQVFDAPATLE
                     YLCEMSGGHPRHLMMFVQAACNELDDLPITRHAAGKAVRRYANALLREVPDAAWDALA
                     HFKTPQRDIPKDDLHQQMMFLLFVFEYMNGAPWYELNPVLRTLERLAR"
     gene            37647..>38224
                     /locus_tag="PKX01_04640"
     CDS             37647..>38224
                     /locus_tag="PKX01_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75443.1"
                     /translation="MPADSPLEAAGNAFRALRRSLLRSRGFAVLVCVCDSLSNRDELI
                     ADLAASLPAVTLHRVDAGDGDCDLLARIVQEFADAPPGPVMILGLERVLADAQAAERM
                     LAALNLSRAEWPTRMAQPVVFWLPRRYLGRLTAGAPDFFDWRSDTLDFPELSAVQLRP
                     FGQREWTFGGDPRLSRAEREERIRELRARISAL"
BASE COUNT         5981 a        11873 c        14343 g         6027 t
ORIGIN      
        1 cgcttggggg gaacgatctt gctcacgcgg agcatgctca tcacgtactg ggccattttg
       61 ggatgccttc gggctgcaaa aacaaaggcg caactttacc cgagttgccc cgctttggct
      121 gcgccggtgc ggcgccaggc tcagttggcc gcttgctgca tctgggcgag gaggacggcc
      181 tgttcggcga gggagaagaa gtaggggtag agggtgcggg tgtgcttggg ggaggtgccg
      241 ccgaagcggg cgatgtggtc gtccatgtgg ctgatggcca gatggagcag caccgccggg
      301 gcattgacgc gctggcagtg gcgcaggggg ccgatctgcc cgaaccccag caggcgctgg
      361 tagaacgggg cgtggcgcgg gttgacttcg atgaccacgt cgtccgcctt gtcgatgagg
      421 cgggcctgga tgtaggccag atgaaagagg ccgccgaggg cttccttggc ctggaccggg
      481 gagtccacgg cgaggccggt gatctcgcag agcaggcgcc cgcgctggcg gaaggcgtcg
      541 agttcgtgac ggtagagcgg gtcggcctgg agcccggccg gggagtcgat gcccaccgtg
      601 atggtgccga agacttcgtc tgcgctggag gtgacgaggg tggtgcggtg gtggttgtga
      661 tgcgggccgt gatgactgcc gaccggggtg agtccgcgcc acgagtacat gcggtcgatg
      721 agcatccgga cggtctgccc gtggcggacc ggatcggccc gttcgagcgt gaagcgtcgc
      781 gggtcctgca gatggacgcc gcgggggggc ggttcgacgc tgtgataatg gctgtggcag
      841 gctctgtctg tcatccgttc gaggggccgt gtcatcggtg gcgtcaggga ttccgcagcg
      901 gaggaggccg gtgttcccgg cgtgggcagg cgagtctgaa ggagccctga cgatatacgt
      961 atacagggtt cggcacgagg cccattttgg tattgagcgc gacagatgca acgaatcgcc
     1021 gaaaattaac attcattccg gccgctgctg gccggcaaga cgggcgtaga gcccgccctg
     1081 ggcgagcagt tccgtgggac taccgtgctg caccacgcgg ccgtggtcca gcacgaagat
     1141 gcggtgggcg cggcggacgg tggacaggcg gtgcgcgatc atcagcgtcg tgcgtccgcg
     1201 catgagctcg tccagggcgg cctgcaccat ccgctcgctt tcgctgtcga gggccgaggt
     1261 ggcttcgtcc agcagcagga tggggcggtc ggctaggagg gcgcgggcga tggcgatgcg
     1321 ctggcgctgc ccgccggaca ggcgctggcc ccgttcgccc agccaggtgg cgtagccctc
     1381 gggcaggcga tcaatgaatt cgtcggcgaa ggcggcccgg caggcggcgc ggacggccgc
     1441 ctcgtcggca tccgggcggg cgtagcggac gttttcgagg acgctcgcgg cgaagatcgc
     1501 cggctcctgg ggcaccaggg ccatgcgccg gcgcagcgcc gcgggatcgg cctggggcag
     1561 gggtacgccg tcgacggcga tctcgccggc cgcgacatcg tagaaacgca gcaggagctg
     1621 gaagaccgtg ctcttgccgg cgccggacgg gcccaccagg gcgacggtct cgccgggggt
     1681 cacggacagg ctgaagtcgt ccagggccgg cgtgtcgggg cgcgacgggt agtggaagcg
     1741 caccctctcg aagtgcagcg cgccgcgggg cggatcgggc aggggccggg gattggccgg
     1801 tacgacgatc tccggtcgga tggcgaggat ctcggcgatg cgttcggtgg cgccggcggc
     1861 ccgctgcaga tcgccccaga cttccgacag ggcccccacg ctggccgcca ccagggcggc
     1921 gtagaagacg aaggcggaca gctcgccggc gctcaggcgg ccttccagca catcgtggcc
     1981 gcccacccac aggatgaagg caatggcgcc aaagacgagg agcagcaccg ccgccaccag
     2041 acgggcccgg ttggcgatgc gctggcgggc cgtggcgaag cccgtctcca cgaggcgcgc
     2101 gaagtcctgc cggtcggcgt cttcgtgacc gtaggcctgg acgatgcgga tctcgtggag
     2161 cgtctcgtcg atgcggttgc ccaggtcggc gacccggtcc tggctttccc gggcgaggcg
     2221 gcgcacgcgt cgcccgtaca gcaggatggg ggccaccacc agggggacgc cggcgagggt
     2281 cagcagggtg agcttgaggc tggtggtgaa cagcatggcc agcccgcccg ccagcagcag
     2341 ggcgttgcgc agggcgatgg acaggctgga accgatcacc tgttcgatct gcgtcgtgtc
     2401 ggcggtgagg cgcgacatca cttcaccggt gcgtccctgc tcgaaccagc ccgggggcag
     2461 gctcaggagg tggtcgaaga gctgctgccg cagatccgcc gtgacccgct cgccgagcca
     2521 ggatacgttg tagaagcgcg tgtaggtggc ggcggcgagg agggcgatga gggcaaaggt
     2581 ggcggcgagg ctgcgattga gccaggccgg gtcgcgggcg gcgaagccct ggtcgatgac
     2641 cgcgcgcagg ccctgtccga ccgccagcat ggcagcggcg gccagcacca gggccagggc
     2701 cgcgacgagc acgcggcggc ggtaggggcg cagacgttgg acgagggctg tcagcatgag
     2761 caatgacggt gcggcgcgcg gcaaaggccg gtactctagc aggccccgtg tgcttccttt
     2821 gggttcccca tgctttccac gcttcccctc actgccggag tgacgctgct gatcgtgctg
     2881 ctcatgttcg ggacctgttt cgcggttggc aaggcgcgtc acacctatgg catccaggcg
     2941 ccggcggtga gcggacaccc cgccttcgag cgcctctacc gggtgcagat gaacaccctg
     3001 gagtgggccg cgatgacctt gccggccctg tggatctgcg cggtctacgt gggcgacggc
     3061 gtcgccgcgg cgctcggcgg cgtgtggctc gtcgggcggg tgtggtacgc gctcggctac
     3121 gcccgcgatc cggccctgcg ggcgcggggc ttcggcattg cggcgctggc cttcggggcc
     3181 ctcggtctgg cggctggcgg cggcgtcatc cggcatttgc tgggggtgtc gtgaggcggt
     3241 ccctgcgccg ggtcgcggtc cttgcggcga tcgcgctggc gggaccggtc ggggctgcgc
     3301 ccgccttgcc cgccctgggc gtggagggcg atggggtgac ggtctccggc atttcatccg
     3361 gcggctacat ggcggtgcag ttccaggtgg cgttttcgcg gattgtgaag ggcgccggcg
     3421 tgctggcggc cgggccctac gagtgcgcgg agggcagtgt ggcgaaggcc ttgacgcgtt
     3481 gcatggcacc caaccgcctg gcgccgccgc cgaccgtggc cgacagcgtg gcgcgggtgg
     3541 acgcccgtgc ccgtgccggg cagatcgacg cgccggcggg gctggcggag gatcgcgcgt
     3601 gggtgctctc cggcggtgcc gaccgcacgg tggagcggcc ggtggtggac gccctggtgg
     3661 ccttctaccg ccactggctg ccggcgacct cggtcgccta cgtacgcctc gacgacgcgg
     3721 cccatggcct gagcgccggc cacgggatga tctcggtgag cgacgcccag gccaatgcct
     3781 gcgccacgtc ggagccgcct ttcatcaatc gctgcggaaa ttttgacgcg gcgggcgagt
     3841 tgctgcgctt cctgctgggc cccttgcagg cgcggggcga cgcgcgggca gggcggctgg
     3901 acgccttcga ccagcggccc ttcgtcagcg gcctggcggc ggacgcgggg ctggcggagg
     3961 agggcctggt gtacgtgccg gacgcgtgcc gcgcgggggg ctgccggctg cacgtggctt
     4021 tccacggctg ccgacagaac ctggatcagg tcggccggcg ctttgcggaa ggtgccggct
     4081 atgccgagtg ggccgccacc aaccggctgg tggtgctgtt tccccagacc gtgacgcgca
     4141 acggctgggc ctggggctcg tggcgctggg tggtcaatcc caagggctgc tgggattggt
     4201 ggggttattc cggtgcagac tacgcgaccc gcacggggcc acagatggcg gcggtgcggc
     4261 ggatgatcgc gcgtctggcc gaggcccccg ggcgctgacc ggcgcgtgtg cgcccgccct
     4321 gcgccgcacg gcgcgcgggt ggcgcctcga ccccgtcact tttccacgaa ggcccgctcg
     4381 atgacgtagt cgccgggcgc gccgatgtgg gccgagacct tgaagccgcg ggcgtccagc
     4441 atgtcgcagc agtccttgag catgtgcggg ctgccgcaga tcatggcccg gtccaccgcc
     4501 ggatcgaggg gcggcaggcc gatgtcctcg aagagcttgc ccgaggtgac caggtcggtg
     4561 acgcggccct gatggcggaa ctcctcgcgg gtgacggtgg ggtaatagat cagcttgtcg
     4621 cggatggcgt cgccgaagta ttcgttctgc ggcaggaaat cctcgatgaa ctcggagtag
     4681 gccagctcgg agacgtaacg gacgccgtgg agcaggacga tcttgtcgaa gcgttcgtag
     4741 gtctcggggt cctggatcac gctcaggaag ggtgcgaggc cggtgccggt ggagaacagg
     4801 tagaggtgct tgcccggcag caggtcgtgc agcaccaggg tgcccgtggg cttcttgctg
     4861 accacgatgg ggtcgcccgg gcgcagatgc tgcaggcgcg aggtgagcgg gccgttgggc
     4921 accttgatgc tgaagaactc caggtgctct tcgtagttgg ggctggcgat gctgtaggcg
     4981 cgggtgaggg gacggccttc cacgtccagg ccgatcatca cgaactggcc gttttcgaag
     5041 cgcagggcgg tgtcgcgggt ggtcttgaag ctgaacaggc tgtcgttcca gtgatggacg
     5101 gaaaggactt tttcggtggt gagtttgctc atggccgcct ccgggcggat gggatgacga
     5161 ggacagtgta acggggcggt aattcgatag aatggattgt ataaatatgt gcgttcgaac
     5221 aaatcgataa gaggcctgcc gcccatgcgc tacaccctgc gtcagctgga gatcttcgtg
     5281 gccgtcgccc gccatgagag cgtgtcccgg gcggccgacg agatcgccct gtcccagtcc
     5341 gccaccagcg cggccctgag tgaattcgag cggcagttcg acacccaggt gttcgaccgt
     5401 atcggcaagg ccctgcgcct caacgagctg ggccgcctgc tgctgcccga ggcggtggca
     5461 ttgctggagc atggccgcgc catcgagggg ctgctgatgc gccaggaggc cttcgggccc
     5521 ttgcagatcg gggcgacgct gacggtcggc aattacctcg ccacgctgat cgcggccgag
     5581 ttcctgcgcc gccatcccca ggtgcgcatc ggcctgcagg tgcacaactc cgcgaccatc
     5641 gtccagcagc tgctgcgctt cgagctggat ctggggctga tcgagggctc ctgccgcgac
     5701 ccggagctga tcgtggagcc ctgggtggcg gacgagttgg tggtgttctg cgcgccgacg
     5761 cacccgctgg ccgcggccgg gcaggcgagc gtggatgcgc tgctgcgtca gccgtggatc
     5821 ctgcgggagc agggctccgg tacgcgggaa accttcgagc aggccatgcg ccacgtcacc
     5881 ggcgcattcg acgtgcgcct cgagctggag cacaccgagg cgatcaagcg cgcggtggag
     5941 agtggcctgg gcatcggctg catctctcgg ctggccctgc gggaggcctt ccggcggggc
     6001 agcctggtgc cgatcgaggt gccggagctg gatctgcgcc gccagttcca gttcgtcacc
     6061 cacgcccgca agtacgtgac cgcgggcatg ggggagttca tcgccctgtg tcggcagctg
     6121 agcgccggcg ccacgcgcag cgacgagatc gagctgccct tcattccgtg accggcgcgg
     6181 gggtcggtcc gaccggcgat tccgtaccga gcaggcggtc tgcagccagg gggccggggc
     6241 cgtggaagag ggtcaccagc agcaggctgc cccacaggat gtgatccttc aggccggccg
     6301 gcgtcaggtc cgggtaggac agcaccgcga cggcgttgaa gacgaacagg gcgaaggcga
     6361 agaagcggcc gccgagcccg agggccagga agaccggcag gatcagctcg gcgccggtgc
     6421 ccatgtaggc cgcgatctcg ggcggcagca gcggcacctc gtattcgttc tcgaagaggg
     6481 cgagggtgct gtcccagctc tgcagcttga ccaaaccgga caggatgaag acccgcgcca
     6541 cgtacagacg gatggccagg tcgaagaggg gcgagaggcg ttgcagctgg gcgacgacga
     6601 cgcggtagac atcgcgggcg gtgaggagga ggttcatggc agcttccttt cgggtgcgga
     6661 ggacgatccg gccagggtgc cggcgcggac gtggtgggtg agggtggccg gcagatcgaa
     6721 ggccgggtcg gtgtcgaatg cggcggtgac ggcgtcgccc agcggctggc cggccgccag
     6781 tgccgcgagc caggcgaact cggctggcgt gagggcttcc acgtggacgt cgtccggacg
     6841 acggacgacc agcaggcagg cgccgcccgc ggccgcagcg gcatcgaccg tgccatcccg
     6901 gtcgggctgg ttggcgcgcc agatggccag ggtcgggtgg tgactggcga gcagccaggc
     6961 ggacggatgc agggccaggc gcagggcttc cggcgcgtcg accgccagcg cggcggggtc
     7021 gaagggcgtt gcgtccgggg cgccggccgc catctcgatg gcccattcaa ggcgcgccac
     7081 gtccggcagc caggcgaggc cgcgggtggg cggatggtcg gcgaggaaat ccggcaggtg
     7141 ggcgccgaag gccgccacgt ccccgtcggt ggaggggacg gcgcggacca gggcgtcggc
     7201 ggtctgacgg aagcagtccg gcccgatcag ccgctccacc accggaaagc tggcggcgag
     7261 ggcgcggcgg taattggcga agagggcatc ccggtagatc gccgcagcgt ccgcagcgcc
     7321 gaggccccgc ggaccggcga tcaggtggtc ggcggaggcg ccatggagca gcgcggcgct
     7381 gaaggcctgc tggagctcat gcagggacat ggcaggcctc caggcagtcg gcggcgcgat
     7441 agacctcggc gaggagctcg gcgaggggcg ggatgtcctg gtcccgctcc agcagggtcg
     7501 gacgcgggcc gaaacgttcc agggcgtcgg cgtagagggc ccacacggcg tcgcacacgg
     7561 gacggctgtg ggtgtcgatg aggcagccgt gggcggcctc gtgaccggcc aggtggagtt
     7621 cggcgaccgc atccgccgga agggcgtcca ggtaggcaag ggggtcgaag ccgtggttgc
     7681 ggctgctcac gtagatgttg ttcacgtcga cgagcaatgc gcagccggtg cgccgggcga
     7741 ccgcggcgac aaagtcccat tcgttcaggg catcggcgtg gaagcggcag tagctggaga
     7801 cgttctccac cagcaggcgg cggcgcaggc gggtctgtac ggcgtcgatg cgggcgcaca
     7861 cgaggtccag ggcttcgtgt gtgtagggca ggggcagcag gtcgttgaga tggcgaccgg
     7921 cgacgcgggc ccagcagagg tgctcggaga cgaggaaggg gtcgatgcgg tcgatgaggg
     7981 cttcgaggcg gtccaggtgg gtatcgtcca ggggatcggc cgaaccgagc ccgagcccga
     8041 caccgtgcag gctgaccgga tagtgactgc gcagacgctc gagcagaccg atgccgctgc
     8101 cgccgggggc gaagtagttt tcgctgtgga cttccagcca gccgaccggc gggcgggtcg
     8161 cgaggatctc gtggatgtgc ggggtgcgca ggcccaggcc ggcgagggcg cggggcgagc
     8221 ggagcggggc gcttgagggc atgaggcgac gtccggagag agtccgtggg cgggcggggc
     8281 cgtgacggcc gtccgcccgc gctgggaatt acttcttggc gtccgccacc acgacgccac
     8341 cggcgatctt ttcgcaggtg cccttgggca cgtagatcca ggcgtccttc tggttgtcct
     8401 tggacacggt gccggcgcag gcgctggagg cggtggcgca gtcgttcttg ccggccttga
     8461 cgacgccggc gcacttttcg ttgtcgccct tggcggcgtg ggcgggcatc gcaccggcgg
     8521 cgaggccgag ggccagcagg ccggcgacgg cggcgcggac ggtctgggtg gcgggggcgg
     8581 tgcgggtgat cgtggtcatg tcggttctcc tgatggaatg aaggcttcgg tttcgagcgg
     8641 ggcgcccgtg cgccgccgtc gtgggaaaag acagggggcc ggccgggatc cttacgaccg
     8701 gatgcgaaaa atattttttt gcccatgccg taagctttgg cgagggtgga cggtctcttc
     8761 ccatgcgaga cgccatccgg gtgtcgcgta agacccaacc ctttcaggag actgatcatg
     8821 acttcccgta cttccggtat tgccctggcc accgctgccg ccgccctctt cgccaccgcc
     8881 atggccgcgc ccgtcgccca ggccgccgac gcgcccttcg tgaagtgcac cggcatcaac
     8941 agctgtaaag gcacctccga gtgcaagacc gccaagaacg aatgcaaggg ccacaacagc
     9001 tgcaagggca tgggctgggt gaagaaggct tcggcagacg aatgcacctc cgctggcggc
     9061 aaggtgatga agtaagcacg gccgaaacag ccatccgtac cggacaccgg ccctcgcgag
     9121 ggcgccggtg tccggtcctg aattccgcgg gcgtgtgtgt gcgcgcgagc gggaaagaaa
     9181 gcgaaagggc gctgtgtgag cactgcatcg acgatcgtcg cgggcttcgg gctggggctg
     9241 cgtacggatc attaccggga tttcaccgag ggccgtccgc ctgtggactg gctggaaatc
     9301 ctgagcgaga actacatggt gcccggcggc aagccgctgc actttctgga ccgcatccgc
     9361 cgggactatc cgatggtgat gcacggcgtg tccctgtcca ttggcagcgc cgatccgctg
     9421 gatcgggcct atctggccga tctgaagacg ctggtggagc gggtggagcc ggcctgggtg
     9481 tcggaccatc tgtgctggac cggcgtggat cacgcccatc tgcacgatct gctgccgctg
     9541 ccctatacgg aggccgcgct gcgtcatgtg acggagcggg tccaggcggt gcaggatgta
     9601 ctcggccgcc gcctggtgct ggaaaacgtg tccagctatg tggcctggca ggcggacgag
     9661 atgagcgagt gggattttct ggcggaagtg gcgcggcgcg ccgactgcga gatcctgctc
     9721 gatgtgaata atgtgtacgt gagtgcccgc aaccacggct tcgatccgcg ccacttcatc
     9781 gacgcaatgc ccgctgcgcg ggttcgccag atccatctgg ccggccacga ggacctgggc
     9841 gacgtggtca tcgacaccca cgaccacccg gtgtgcgacg acgtgtgggc gctgtacgcg
     9901 tatacggtcc gccggctcgg gctggtgccg acgatgatcg agcgggacga ccggattccg
     9961 ccgctgcaga ccctgctggc cgaactggat ctggcccggc gtaccgcgca aggcgccctg
    10021 caggaggcgg tggcatgacc cctctggcga atcagcagcg ggccctgcag acggccatcc
    10081 tgagcgacgt ggcgcccgcc ggcctgctcg ccggtcccga cgccgcacgg cgcttttcca
    10141 tctatgcaga cgcctaccgg gcacgcctgg cggcggcgtt gcgggacaac tatccggtgt
    10201 tgcaccgggt gctgggggac gaggacttcg acgcgctggc gcgcgcctac ctgcacgcgc
    10261 atccgtcggt ggccccgtcg atccgctggt ttggtggtgc gttggcggac ttcgtgcagg
    10321 caacgccgga tgcccttccc cacccggcgc tggtggacct gatccgtctg gaatgggcca
    10381 tgcgggcggc cttcgatgcg gaggacgccc cgcccatggg ctttgcgacg ctcgctgcgc
    10441 tgccgccgga tcgttggccg gccctgcgcc tgcgtctgca tccctcggtc tcgcgccttg
    10501 ccctggagtg ggccgtggag cccctgtggc aggcgcttgc tcccctggag gacggacagt
    10561 cgcccgagca ggagccgcca ccacccgagc cccatgtcca tggtctgatc gcctggcggc
    10621 aggggcttac gccgtgctgg cgtagtgtgg aggcccttga aggacatctg ctggctgcgc
    10681 tggcaggggg agcggccttc gcggatctat gcacccttgc ggcggatgcg ggcgaggagg
    10741 ccgccgcgca ccaggtggcc ggcctgttgc gccgctgggt ggacgagggc ctccttcagg
    10801 cgtgtgacgc gctgatgtcc acgccgtgat gacgaagtga caactaccca cacggggcgt
    10861 gggcaagcct gtggacaaac tgtgggggag gcgggcaggt ggcggacggg aaagggattt
    10921 tcaggtctgc ctaaaaatag tgcaaatatt gcgaaagccg atagcggata gtttttgcct
    10981 atccacactc cgtggggaat actgtgtgga caagatggga gtcatgccct caagtccctg
    11041 atttattgga atctcgtgct gctgaccgtt tcaggtcagg aacgtcgtga tggcagcgct
    11101 gcccggtttt ccccagcatt cgtgggcaag catgtggaca agctgtgggg gcgtcgggca
    11161 atcccttcat cgcaaagggg ttttccagac tgccgcaaaa tggggcatcc ataccccgcg
    11221 cggtcggcgg tgcgcagggg gcgacgctca gggggtgagc tggtcggcca gatcggtcag
    11281 gctgacggcg gtcaggtcca ccgggacggt cggcatccgt tcaccggcac cctgggcgaa
    11341 ctcgtcgggg cgggcgatga acgccgtgcg caggccggcg cgggcggccg cgtccagatc
    11401 ggaggaatgg gccgccacca tcagggtctc ggccggtgtg aggtcgaagg cgtcggcggc
    11461 ggccaggtag accgccggct tgggtttgta gtcccgcgcc acttcggcgc cgaggatggc
    11521 gtcccacggg aagtcgttgc gccgggccag gtccaccatc agcgagatgt tgccgttgga
    11581 acagggcgcc agacggtagt gtcggcgcag gcggtgcagg cccggcgcga cgtcgggcca
    11641 ggcgtcgagc cggtgccagg ccaggttgag ggactggcgg gtgctttcgt ccacggcatc
    11701 cagaccgaag tcggcgagca ccacgtccag gttgtggcgg tgcaggcggt cgagcttgca
    11761 gaagggcagg cgcccgctgc ggaccgcttc catggccggt tggtattggt cgcgccaggc
    11821 gtcggcaaag gcgagggcgt ccacctgaac gccctgggga gccagatgcg ccgtgacctc
    11881 acgggcgacg gaggtgcgcc agtccaccag ggtgccgaag acgtcgaaga tcagggcttt
    11941 cacgatcatg gccgggggcc tccgggtgcg atgggctgga aggccgcgac aagggtgtcc
    12001 acggcggcca tgggcggtgc gccccggagc cggatcatgc cggtggggac gtgggcgatg
    12061 tcctccggta cgctttccac gacgagatcc ggggcatgca ccgactgggc gacgactcgg
    12121 cgcggcatca gcgtccaccc cagccccacc gcaacacagc cgaggatgcc ttcgacggtg
    12181 ccgaattcca tggcgtcgga gagggcgtgg cccatggccc gctgccaggc cagggcgcgg
    12241 gtgcggtagg cgcagccttc gcggaacagg atcaggggca ggtgcaccgg tcgggtgccg
    12301 atcgcgcaga tctggaccag ctcttcgtcc accagcgggc agaaatccag cgcgggatga
    12361 tccaccgggc ccgccaccat ggcgcagtcc agcttgtggt cgagcaccct ttccacgagg
    12421 acggcgctgg gggcggtctc gatccgcagt tcaaccgccg gatgatgccg ccgcacggat
    12481 ttgagggcgg ccggcaggtg cagggcggtg aaggtctcca tggcgccgat gcgcagctcg
    12541 ccgacgcttt cgcccacctg gcgcagggcc gcgctggttt cccgttccag ctggagcatg
    12601 cggcgggcgt agtcgaggag gacgcggccg ggcggggcga gttccagtcc gcggcccttg
    12661 cggaagaaga gctcggtgcc gagttcttct tcgaggcggc ggatgcggcc ggtgacgttg
    12721 gactgcaccg tgttcagctt gcgagaggcg gccaggatgc cgccttcctc gacgacggcc
    12781 tcgaaggtgc gcagggcgat gagttccata tatctctata gaagaatgga tgcttcttga
    12841 caaatcattt gaatggatag attacacgcc ttaatgtggg aacatcaacc cagatggaga
    12901 aaaccatgag ttcccgacga gagcgctttc aggtgatggg ggcaggcatc tgcagtctca
    12961 tcctggtgct gggggtggcg cgcttcgcgt acacgccgct gctgcccttg atgcagcgcc
    13021 aggccggcct gggcgtgggc gaggccggct tgctcgcggc gatcaactat ggtggttacc
    13081 tgagcggggc gctgatcgcg tccctgatca gcgatctggt gctgaaggat cgcctgtacc
    13141 gcatcgggat ggtcctggcc atcgccagta cggccctgat ggggctcacc acggatgtga
    13201 cggtgtgggc ggtgtcccgc ttcgtggcgg ggctgagcag cgcggcaggc atgctgctcg
    13261 gcacggggct gatcctcaac tggctgatcc gtcacgatca tcggagcgag ctgggcatcc
    13321 atttttcggg tatcgggctg gggattgccg gctgtgcagc ggcggtgctg ctgatgagtc
    13381 cgtggttcga ctggcgcggg cagtggctga tcttcgcgct gctgggcggc atgctgctgg
    13441 tgccggccct gcgctggctg ccggcgccgg atccgagccc cttcacgaag agcggcgagg
    13501 cgctgcggga ccagccgccg gggcgactgt tcatgcgcct cttcatggcg gcgtatttct
    13561 gcgccggggt gggttatgtg gtgagcgcca ccttcatcgt ggccatcgtg gatcgtctgc
    13621 cggggctggc cgggcaaggg gcactggtct tcctggccat cggtgcggga gccgcgccgg
    13681 cgtgcatcgt gtgggatttc gtcgcccgcc gcaccggggc gctgaacgcg ttgatcctcg
    13741 cggcggtgtt gcagatcgcc ggcatcctgc tgccggtggc ggtgggcggc agtctggcgg
    13801 cgtttgtcgg ggcgctgctc tttggcggga ccttcatcgg catggtgagc ctggtgctga
    13861 ccatggccgg gcgctattac ccgacccggc cggccaagat gatgggcaag atgaccctct
    13921 cctatggcgc agcccagatc ctcgggccgg cgctcaccgg ctggttggcc ctgcattggg
    13981 gcagttacac cgcgggggtg tatctggcgg ccgggatgat ggcggtgggt acggtgctgc
    14041 tgctggtgct gaagggcgtg gagcggcgcg acgcggcggc cttgcgggcg gtggcggcct
    14101 gagcggcggg tcgggtcggg ttagaatccg gccttccccc cagcctggtc cctgccatgc
    14161 ctgcgcccgt cgaatacacg atccgggcgg cccatccggc cgcccatctg tttgaagtca
    14221 ccctcaccct ggccgagccg gacgtggccg gccaggtctt cagcctgccg gcgtggattc
    14281 ccggcagcta catgatccgg gactttgccc gccacatcgt gagcctgcag gcggaggcgg
    14341 gcgggcgggc ggtggcggct gtcaaggacg acaagcacac ctggcgcctg ccggccgggc
    14401 tgcgggggcc actcacgctg cgctacacgg tgtatgcgtg ggacttgtcg gtacgcaccg
    14461 cccatctgga cgagacccac ggattcttca acggcaccag cgtgttcctg cgtgcccacg
    14521 gtttcgagga tcgaccccaa cgggtgcgtc tcctgccgcc cgcggggctg gaggcggccg
    14581 gctggcgggt ggcgacgagc ctgccggagg cgtcgccgga agtcgggggc gccgagcgct
    14641 acggattcgg tgtctatgag gcggacacct acgatgcgtt gatcgaccat cccgtcgaga
    14701 tgggctgctt cgatctgttt gagttcgaag cctgcggcgt gccccacgcg gtggcgatca
    14761 ccggccgcca cgactgcgac ggcgagcggc tggtggcgga tctcaagccg gtctgcgagt
    14821 ggcagatccg gctgttcggg gaaccggcac cgatggaacg ctatgtgttc ctcgtgatgg
    14881 cggtgggcga cggttatggc gggctggagc accgggcgtc cacggcgctg ctgtgcagcc
    14941 gcaacgacct gccccatcgc ggcatgggcg ccccgaccga aggctaccgg cagttcctgg
    15001 gcttgtgcag ccacgagtat ttccacacct ggaacgtcaa acgcatcaag cccgcagcct
    15061 tcgtgcccta tgagctgggg cgcgagaact acacgcggct gctgtgggct ttcgagggct
    15121 tcacgtccta ttacgacgat ctggccctgc tgaagagcgg gcggatcagc cgtcgcgaat
    15181 atctggatct gctggcgaag acggtgagtg ctgtccacaa gggcagcgga cgcctgaagc
    15241 agagtgtggc ggagtcgtcc ttcgaggcgt ggacgaagta ttaccggcag gacgagaaca
    15301 gccccaacgc catcgtcagc tattacacca agggcgcgct ggtggccctg ctgctggatc
    15361 tgcgcctgcg gggcgagagc ggcggggcgt gttcactgga cagtctcatg ggtgtgctgt
    15421 ggcgagacca tggccagacc ggcgtcgggg tggcggagga ggggctctac gcggccgccg
    15481 ctgccctcgg cggcgcgtcg ctcgggcgct ggctgcgtaa cctggtggag ggaaccgacg
    15541 agctcccgct ggaggacggg ctgcggcgcg tgggggtgaa gctggcgtgg gaggcgtcgg
    15601 tgaaatgccc gggaatgggc gcacggacca ccgtggaggg cgaatccgtg aagctcgcga
    15661 atgtgttcga cggcggcgcg gcccaggcgg ccgggctgtc ggcgggcgac gtactggtgg
    15721 cgctggacgg tctgaaggtg accggcaaga cgctggacgc gttgctggcc cggcgcatgg
    15781 ccggtgagac tgtccaggtc catgccttcc ggcgggacga attgaagacc ttttcggtga
    15841 tcttgcaggc gccgttaccg gatacggccc gtctgacgga agcgccccgc tgctcggccg
    15901 cggcgcgggc cttgcgggaa gggtggctgg gcgcttgaga cgacgggggc cggatctccg
    15961 gccccgcggg gtgctgggcg ggcgctcagg cgggtaccgg ccggcggacg gcgaataccg
    16021 ggccgtcttc cacgtaccgg tggagcaggg tgccggcgcc gatgctggtg gaccaggtca
    16081 gcacaaaggt ggtgacggcc agggcaggct gatggccggc cacattggcg aagagggtgt
    16141 tgaagaccag actgaccggg tagtggatca ggaaggtgga gtaggagatc ttcgacagga
    16201 agaccacgat gccggtcggc gagatacgct ggaacagggc atgacggcat gccagtgcca
    16261 tggacaggga ggcgatgagg gcgaccgcga tgcggacccg gaaatccacc accagcacga
    16321 gggttccagc ggcggccagc agcaccagcc agccatggga actgcgaccc gcggcccagt
    16381 aggcaaaggc gcccagggcg tagctgccga agaagtacag gccccagctg tcccagtcct
    16441 ggtcgagatt gaagtacagc agcgacgcgg cggcggcggc cgcgaccagc atcatcaggc
    16501 gttgggtggg gctggcggca ccgcgcccgc tgtgggccag ccagaccaga ccaaccatca
    16561 gggcgtagag ctggaaatcg acggccacgt accacacgcc ggcggacagg ctgtcatagc
    16621 cgagcaggct gtgaagcagg aagacgtggg ccaggaactg gcggatcgtc ggaatggccg
    16681 gggtggcgtc gtcgtgcatc caggtgcggg cgacggcggc acaggccagg gcacaggtga
    16741 cggccaccag atagggaatg gcgagccgca tgtagcgccg ggcgatcagg gtgaggggct
    16801 ggacgcgggt cgggacgcca ccgggcgcca gtccgcgggc cgagaggaag ccggcggcgg
    16861 ccaggaatac ggggactgcc agccggccgt attcggcgat ccacgcgacg agggcgggca
    16921 tcacgtcagc catggcctcg gacaagggcc cgtagatgac cagatggtgg aggacgatga
    16981 gttgggacgc gcaggccttc agaagatcga tgaagggcat ccggcgtgca agtacgatgt
    17041 gacccactag agtaaattgt gtatcaaatt ctttgatttt acacggaaac atgccggact
    17101 gtcatgactt ttccgtacgg tacggtattt tttgacgcac tgccgcatct tcggcaggcc
    17161 gcttgagacg ggcaatccgg tacagatagg tgccgaacag ggttgccgcg gcgatgttgc
    17221 tgaggaccga cgcctgccag aatccggcca ctccctgggg cggaaagggg ccgatgccgc
    17281 ggaaggccag gaggtatccg ccggccagtc cgatgcccca gaagcacagg gtgtgcacga
    17341 gcatcgggcc gaaggtgatc ttgtagccgc gcaaggcatg ggcggcgacg gtctggatgg
    17401 cgtcgaagaa ctggtacagg cagatgtaca ggatcagggc gtcggccagg gtgcccacgg
    17461 ccgggtcggt ggtgtaggcg gcgacgacgc ggtcccggac cagccagacc aggacgctga
    17521 cggtggtgga gagtccggtg gcgaggagga tcccgcacca ggcggcctga cgggcgcgga
    17581 cgtcgtcgcg ggcgccggcg gactgtccca ccaggacgag ggtggcggtg gcgagggcca
    17641 ggggcagcat gtagatcagt gcggaaagat tggcgacgat gcggtgaccg gcgaccacgg
    17701 cggcgcccag ttcggccacg aacagggcga tcagtgtgaa ggcggtgatc tcgacgaagc
    17761 tggaaaagcc catcggcagg ccgaggcgca ggagttcggc cagtgccgcg gggcgcggcg
    17821 cctgccagcg ggtgaagagg cggtaacggg cgtaggcggg attgcgcagc aggtagagca
    17881 tcgcgcagcc cagggcgaac caggcgatgg tggcgctgga cgcggcgcac ccggtgccgc
    17941 ccaggggggc gaatcccgcg tggccgttga tcaggagcca ggagaggggg atgtgcaccg
    18001 acgtggcggc cagactgatg agcatcagta cgcgggtccg gcccagggca tttgcgaagg
    18061 cgtagaaggt gcggtacagc agcaccgcgg gcaggcccca ggcgatggtg gagaggtagt
    18121 cgcgggactt cgcttccacc gccgggctca tgtgggacag ctgcatgagg cagtccggat
    18181 ggcgcaggaa gaggaccgcc ggcacggtca ggaggagggc cagccagaag ccctgctgca
    18241 ggtccggacc gatggcctcg tgccgtcctg cgcccacgtg atgggcgacg gtgggggcga
    18301 cggcctggag gatgcccacc atgagcatcg cgatggcaat gtagatcccg ctgccaacgg
    18361 ccacggcggc cagatcctcg gtaccgtaac gtccggtcag cacggtgtcg ctgaccatct
    18421 gggtcatgga cgagagctgg gcgatcagga cgggccaggc gagctgaagg atgcgtcgcg
    18481 cgagggacgg ctcggctgaa gggatgcgtg gggcggcggg ttcttggggg gtgggcatgg
    18541 gcggcgagtg taacccgcgt gccggtcgga cctcatgcga aatagccgtg gaggtaccag
    18601 ccatatgggg gctccagatc ctgaaagagc cagaccaggc tgtcgtcgga gcgccgcgcg
    18661 acgtagtagt cgcgccgcac gccggcgccg tcccaccagc cgctttcgat gcgttccggg
    18721 ccggcgagga ggcgttccgg ggcgggcgcc gttacggcac ggggccgggg gagcagccac
    18781 aggggacggg ggccggcggg ggtgtggaca tcagcgggcg cgtgctcccg ccggggcgtg
    18841 ccccgtcgcc aggcctgctc ggggcgatga tcggcatggg gtgcgcaagt gaatacggct
    18901 tcgtcgccga ggcgggcgcg cagggtggcg aggagcaggg ccgggtcgct gcggggcccg
    18961 ctgggggcat cgaacagatc ggtggcggac ggggccagcg gccggggggc ttcggtctcc
    19021 aggcgcaagg cctcgacggg ggccgtgagg aggaggcggg tcaggtgttc gcgggccagg
    19081 agctggcagc gggacgggtc gttggccagc ttgcccagaa tgacgggcaa ggtttccacg
    19141 cccgtgccgg cgtattccag gaccagctgg aagcgttcca ccccggcctg gcgggcgctg
    19201 agccagccgc tcaggccggc gagcaggcga cgcaggccga agagcagcat gtccacctgg
    19261 tcggtggggg ccggcagggg gaggcgggcg ctgtagcgtt ccggtgggac gaagctcggg
    19321 cggggatcag gtgtccggcc ataggcccgg tcaagacagt cggtgagggc ctgggcgtgg
    19381 cggcgggcga gcccgtggcg ggacaggtgg cggagcgctc cgagccgctg cagccccagg
    19441 ccggcgagga gttccaggac gtcggcgccg ggtcccgagg catcgtccag gacggtcagg
    19501 ggaagcgcgt cgagcgttgc ctgatcgtcg gtgagactgc cgacgccagg ggaaaggccc
    19561 tggcgtgcct gccagcgggc ggcgaggggg gtcgccgctg caccgagccg gccggtgtag
    19621 ccgagttcgg cgagaccgtc gagcagggct tcagcgaggc gtgggagacc gccgaacagg
    19681 cgcaggctgc cgcgcacttc caggaggacg gcgcccggtg ggtccaggct gagggtgccg
    19741 gcaaagcggc cagcccaggt ggcgatctct tcgagcgcag cctgctcgag atcgggatcc
    19801 cgttcggcaa aagtgaggcc gggggctagg gccagagcac tggcgcggct gatccccggc
    19861 gccacgccgc atgccattgc cgccggattc gccgccacca cccgtacgcg ggggcggggg
    19921 gtgaggaggg cgagcggcgt ggcatcagca tgtccccgcg tgaagacctg gagggccaga
    19981 tcgggcaggt agagggcgca ccagagcatg ggggcggggg cgcgggggga ccgggccggg
    20041 cagttcgaga gcgagggtgc cggcgaagag ggggccgcgc cgtttcagga tgcgtatctc
    20101 cacgccaccg acgcggggac gcagggccag gcgcagcacg gccggggacg cctgggtggc
    20161 gcaatgttcc gggcggatga ggaacagggg ggtggcgctg tcctcggagg ccagctgaag
    20221 gcggcgcagc gccgccatgt cggttccggc gagccacatc aggacggctg tgcagctgcg
    20281 ggatgcaagg gcctgacggg cggcccagcg ggcatgcgcc gggctttcgg tctggacgaa
    20341 caggatgcgt ccgaggggca ccccgcccgc tgcgagggcc ggggcatagg ggtgcagggg
    20401 cggcgcgatg cagacgctcc agtgatcctc ggcggtgcgg gcgagcaggg ggagcagcag
    20461 tccgagttcg ccgatgcccg gacggggaat caggagttcg ctgagggcgg cgttgggcca
    20521 gccgccaccg ggcaggcatg cgtcgagatc agggaagccg ctggggatgc ccgcttctgc
    20581 cggcacggcc agacggtcgc cacgccagac caggtggcct ggcaggctgt cgacggcgtg
    20641 gaggcgggcg gaggcggaca tggtgtgtca ggcgcggagc gtgctcgatg gttggcgatg
    20701 tctgtgatgc gggcggatca aatgcttcag aacgattggc tgacgggctc tgtccgttct
    20761 gccgggccat tctccacacg cggggcgtgc agggccccgt gagggatgtc agaccggctg
    20821 agcgcggtcg gcagcgatca gagcggctca ctgcggatca ggccgaccgc aagcccttcg
    20881 atttccagga ggtccctgcg ggtgtcgacg acgatgggtt cgaaatccgg attggcgggg
    20941 aggagttcga caatggggcc gctgcgggac aggcgcttga cggtgacctc gtcgtccacc
    21001 cgggcgacga cgatctggcc gttgcgggct tcggccgtgc gatggaccgc cagcaggtcg
    21061 ccgtcgagga tgccggcatc gcgcatgctc atgccgcgca ccttgaggag gtagtcggcg
    21121 cggggggaga acagggacgg gtccacctgg aaacgcccct ggacatgttc ggctgccagg
    21181 atggggctgc cggcggcgac ccggccgacc aggggcaggc cgagggcttc ggtcaggcgg
    21241 atgccacggg cgcggccttc ttccagggaa atgacgccct tggcggcgag ggcacgcagg
    21301 tgggtctctg ccgcattggg agagcggaag ccgaatgcgg tacagatctc cgcccgggtg
    21361 ggaggacgcc cctcgtgctc gacggtgtca cggatcagct ggaggatttc ctgctggcgg
    21421 gcggtgagtt tttcggacat ggcagactcc ttttgtgtaa ttttatacag cttgtgtgct
    21481 ttggcaagcg gtttatgcaa ggccgccact ttccggccgg cagaccccgg acggcatgtg
    21541 acaaacgtca aacaatgcgg acaaagagct tgagcccctg cgtgacttgg tctatccatc
    21601 aaggacagcc cccgcagcgg ccagacgggg gatccacaac gacagtgcgc gtggcccggc
    21661 aggccacttc caaccccgga ggagacttca tgctggtcag tccgtccaac ccgaccgccg
    21721 gtagagttat ccaggcgcct cggttagaat cgcccggcgt acccgaagcc cctttcgtgg
    21781 gtcagcccgc accgcttttt gccctgccgg atgcagatat ggaaatcttc gatctcgcgt
    21841 cggcgctcgc gtcccatcat gtggtcctgt acttctatcc ccgcgacaac acccccggct
    21901 gcacccgtca ggccattgat ttcagtgacc acgacggaga ctttgcccgg gagcgctgca
    21961 ttgtcgtggg cgtgagtccg gacgattgcc tgacacatgc ggatttccgg gaccatcatg
    22021 ggctatcggt gcaactgctt tccgacgaag actacgaggt atgcaaattg tataacgtct
    22081 ggcgctcgca ggaggtggac ggcgtgatga aacagggcgt tcgccgctca actttcatca
    22141 tttcgtgcga tggtgtgctc cagcacgtgc tgcacgacgt caacccacgc ggccatgctg
    22201 cggaagtctt caagctggtc cagcaactca acacaaggaa attcaatgca aatcgccaag
    22261 aacacggtcg tcacgcttga ctatcaggtg cgtgatccgg acggcaacat gatcgacgac
    22321 ggtgctcacc cgctggtgta cctgcatggc ggctatgacg gcatcttcac cgtcatcgag
    22381 gaggccctgc agggcaagaa cgtgggcgag cagctgaacg tcaagctgca gcccgacgat
    22441 gcctttggcg aatacgacga agagctggtt ctcgtcgagg atgcatccct gttcccggac
    22501 aacatcgaag tgggtatgtc cttcgagcgc gtgggcgagg acggcgagga cgagcagatc
    22561 taccgcatca ccgacatcgc cgacggcaag gtcgtggtgg acggcaatca tcccctcgcc
    22621 gggaccgcgc tggtcttcga catcaccatc aagagcgtgc gtgccgcgac ggccgaagag
    22681 atcggtcacg gccacgtgca cggcgaaggc ggtcatcacc actgatccgc aacacggtgt
    22741 gccggaaccc gaagggatcg accggcaccc ggataccctg ccgacgggcc gctctggcgg
    22801 tccgttttct ttgcttcgga cagatcgatt gaagaatcca tgaaaggtca gtccctgctc
    22861 caggtcgatg gattgcgggt tgaactgcgt gccggcggac ggcgtgtgta tcccgtggat
    22921 ggtgtgcatg tgacggtgcc ggcggggcag accctggcgc tggtcggcga gtccggctgc
    22981 ggcaagtcca tgacggccct cgcctgcatg gggctgttgc cacggggtgg gcgcatcacc
    23041 gcagggcagg tgaagctcgc cggcgaggcg ctcgagcgtc tgccggaggt ccgcatgcgc
    23101 gatgtgcgcg gacggcgcat ggcgatgatc ttccaggagc cggcgagcag cctgaatccg
    23161 gtgatgaccg tcggtgacca gattggcgag gtgctggtcc gtcatcgcag cctgcacggc
    23221 cagccggcct ggaacgaggc cgagcgtctc ctcgcggcgg tgggaatccc cgatccgcgc
    23281 cggcgcctgg gcgaataccc gttccagctc tcgggcggca tgaagcagcg ggtgatgatc
    23341 gccatcgccc tggccggcga gccggagctg ctcatcgctg atgaacccac cactgcgctg
    23401 gacgtgacca tccaggccca ggtgcttgat ctgctccgca cgctgcaggc acggcgcggc
    23461 atgggcatgc tgctgatcac ccacgatctg ggcgtcgtcg cccgcatggc ccatcgggtg
    23521 gccgtcatgt acgcgggcga ggtggtcgag gctggcgaac gggaggcctt cttcgcggcg
    23581 cccctgcatc cttattcccg caagctgttt gccgctttgc cggatgcggg gcggcgcgat
    23641 catgccctcg agaccattcc cggtcaggtc cctccgctgg gggcacgcct gcgcggctgc
    23701 cgttttgccg agcgctgtcc gcaggtccgc ccgcgatgtc gggatgcgca gccggccctg
    23761 cacgaggtgg acggacgtca ggtgcgctgt catctttacg acgcggaatc cggcctgagc
    23821 tggctgtccg ccgaggcggt gccggcagga cgcgcagcca cctcgccgga cccccggccg
    23881 gtgctggagg tggatggatt gaaggtccat ttcccggtcc gcaagggggt attccggcgg
    23941 gtgaccggac agatcaaggc cgtggatggc gtcaccctga gtgtggcggc aggcgagacg
    24001 gtcgcccttg tcggcgaatc cggctgcggc aagaccacgg cgggcaaggc gctgctccag
    24061 ctggtgcccg ccagtggcgg cgcggtccgc tacgatggtc gtgagctgct cacccttgcg
    24121 ccggccgact ggcggccggt gcggcgggaa cttcagatga tcttccagga tcctttcagc
    24181 tccctcaatc cgcgcatgcg cgtgggcgag atccttgaag agggcatgcg ctccctgggc
    24241 gtggaacctg atgccgctca gcgggcgctg cgcatcgagg ccctgctgca gcgggtcggc
    24301 ctggatccgt ccatgcgcga ccgctttccc cacgaatttt caggggggca gcgtcagcgc
    24361 atcgccattg cccgggccct cgcggtgtcg ccgcggctga tcgtctgcga tgagccgaca
    24421 agtgccctgg acgtctcggt gcaggctcag atcctcaatc tcctcaagga tctgcagggg
    24481 gaattcggct tgtcctacct gttcatcacc cataacctgg cggtggtgga ttatctggcc
    24541 catcaggtgg cggtgatgta cctggggcgc atcgtggagc gcggtcgggc cgaggaggtg
    24601 ctgcgggatg cgcgccaccc ctatacccgc atgctgcttg cggccgtacc gaaggtgtcc
    24661 gggccggttt tgacggcgga gcagggggcc ggtgaggcga tcgatctgcc gtctccgctg
    24721 agtccgccgg caggctgtca ttttcatccc cgctgcagcg aggctacggt cgcgtgccgc
    24781 gagatctacc cggacgagac ccgcctgtcg cccacccggg tggtgcgctg ccatctgtac
    24841 gcatcgtgac cgggcggcgt tgaacgacag gcaggggttt gacctctgcc gcgtgtcccg
    24901 ccggcgctgg cggcacgggc agggcgcccc gggtgtcgtc ccgactgccg ttcagcggcg
    24961 cttgtgtggc gctgccggtg cagccttggc aggcggcgca gacttggtcg ccggggcggc
    25021 ggccctgcct ggagcaggtg caccgcgatg ggcgcctgcc gggggcggcg tgaccaccac
    25081 ccggcgttcc accaccatcg gccgtccgtt gtggccccgc accacggtcc gctggatcag
    25141 ggtgcgtccg gccgggggcg gctcgacggc accggcctgg ggatgggtcg gcagcatatt
    25201 ggccagagcg atgcgctcgc ccggcgcctc ggacggcacg atcaaggtat agccactgcc
    25261 gatgcgggcg cggggcgaca ggccattgac ctggcgcagg cggctcgacg agatgccgaa
    25321 gcggtcggcc accccttcga ggccttcacc ttcctggagt tcgtaggtgc gccaggtggc
    25381 cgtggagggg tcgtgggttt ccaggttgtt gaggaagatc tccagctttt ccgccggaat
    25441 caccagggat tgggtgccgt tcttgggcag gaccgggcgc ttgtaacccg gattgagggc
    25501 aaggatttca tccaccggcg tctcggccag gcgcgccgcg gtggccaggt cgaggctcac
    25561 gcggctctcg acggtgacga aatagggccg gttggcgatg ggcggcaggg tgatgccgaa
    25621 gagctccggc tgggcgatga tgttcttgag cgcctgcagc ttgggcacgt agtagcgggt
    25681 ttccccgggc atgttcagat actggtattc ggtaggcagg ccggcggcgc ggttcttggc
    25741 gatggcccgg ccgacggcgc cttcgcccca gttgtaggag gccagcgcca attgccagtc
    25801 gccgtgcatc tcgtagatgg tctgcaggta gtcgagggcc gcgctggtgg aggccaccac
    25861 gtcgcggcgc tcgtccaccc accagttctg ttccagcttg tagtttttgc cggtggacgg
    25921 gatgaactgc cacagaccgg aggcctgggc cggggacagg gcggtgggat taaaggcgct
    25981 ctccaccatg ggcaggaggg ccagttcggt gggcatgccg cggcgttcga gttcctcgac
    26041 gatatggaac aggtagcgct ggccgcggga gaacatctgg cgcagggcgc cgggacggtt
    26101 gatgtaatag atctgccggt ctgtcaccag gtcggtgttg aggtccggca tggaaaagcc
    26161 gcggcggatg cgatcccaga gatcgttggg ctcgatggtc aggtcgacgg ttggaacctt
    26221 gacatgctgc tcgcggactt caaaggcctt gggtgcctgc aggcccacgg catcggacag
    26281 ggacgtggcc tgggccagtt gcagcggcac atcgggcacg ccgtcagcca gggccgggcc
    26341 cacacccacg agcagcaccc acagcgcgat aactctctga aacattgggt ttcgccgcgc
    26401 agggacaaag ggcggattct atcccccggg ggcaggaggg tcaaaccggc cccggtgcca
    26461 ttggtccacc ggcgagccat gaagggagag ggggacggaa actacggatt gtggcgtgaa
    26521 tccgttaaag taacgatttc gtacagcggg tgcccagagc gcccgccccg cccgagaccg
    26581 cccgccagga ccgatagccg ccgtgagagc cctgttgatc gaggataccc tgaccagcgc
    26641 cacgctggtg tcgcatcaac tgcgcaatat cggtatcgag ccgatccttg cccgcgacgg
    26701 tgagcggggc atcgagctgt tcaagcagga gcgcccggac ctggtgctgc tcgacgtgat
    26761 cctgcccggc atggatggtt tcgaggtggc ccggcgcatc cgccagctgg aacaggcggg
    26821 cgagtggacg cccatcatct tcctgaccgc ccgcaccggc gacgaagacc tggaaaaggg
    26881 catcgcggtg ggcggggacg actacctcat caagcccgtg tctgagaccg tgctggctgc
    26941 caaggtccgt gccatgcagc gcatcgccca gatgcgctac tccctgctgg tgctgacccg
    27001 caagctcgac gaggccaacc aggagctcac ccgcctgtcg tccctcgatg gactcaccgc
    27061 catcgccaac cggcgccagt tcgacgagac cctgcaacgg gaatggcggc gagcatcccg
    27121 ccagggcaag ccggtgtcgc tgctgttctg cgatgtggat ttcttcaagc agttcaacga
    27181 cggttacggc caccaggtgg gtgacgagtg cctcaaggcg gtggcccgta cactcaagga
    27241 cgtcctgcgc cgcccggccg acatggtggc ccgctatggc ggcgaggagt ttgcagccgt
    27301 gttgcccgac accgacatcg ccggggccct gcaggtggcc gaagccctgc gggcggccgt
    27361 ggaggcgctg ggcatcaccc accgtttctc ccgcgcaacc aaggtggtga ccatcagcgt
    27421 cggggcggcg acggccgtgc ctgcccgcca ggacggcagt cccatgccct tgctcaagat
    27481 ggccgacgag gccctttacg cggccaagga gaacggtcgc aaccgggtgg cgacggtggc
    27541 accgcgggcc gtctgagggc gcccgccggc tcacgcgtca gaaacgattc ttccagccgc
    27601 ggatcgccgc gaagaccgcg gccggcgtgg cgtccccctc gagcccgtga gcccgcgccg
    27661 ctgcgatgac ggcgggctcg cggctgcgca ggaagggatt gacgaggcgc tcgcccccca
    27721 gccggaaggg cacggtcggc agatcgcgcg cccgcaaggc ctgacactca gcgacgcggg
    27781 cggcaatggc cgggttgtca ggttcgacgg cggcggcgaa tttcaggttg gacagggtgt
    27841 attcgtgagc gcagtacacc tgcgtgtcgt ccggcagctc ggcgatccgc gccagggacg
    27901 cgaagagctg cgccaacgtg ccatcgaaga cccgcccgca gccggacgcg aacagggtgt
    27961 cgccgcagaa cagggcgggc gccgcgtagt aggccagatg gccggccgtg tggccgggca
    28021 catgcagaac ctcaaaagtg gcgttgattt ccgggatggt gacgacatcg ccctcaccga
    28081 ccggatgggt caggcccgcg accggcagcc cgtcgcgcgg tccatagacg ggcaccggtg
    28141 ccgccgcggt cagcgccgcg acgccgccgg cgtggtcgtc gtgatggtgc gtggtgagaa
    28201 tggctgccag ctgcagcccc tcggcggcca gacaggcctg tacgggcgct gcatcgcccg
    28261 gatcgaccac cacggcgtag cggcccgcgc gcagcagcca gataaagtta tcgctgaagg
    28321 cgtgaagagg gataatgtcc atccgcgcat tctagcccct gtccccgatc ctgccgatgt
    28381 ctcctgccga atcctttccc ggtctgcacg catggctcga gacaccgctc gggcggtacg
    28441 tcatggactg ggaagcggat cgccatgacc gggtcgtggc cgacatcttc ggctacaacg
    28501 cggttcagct gggcattccc gaattcgatt tcctgcgggc caatcgcatg cccttccgct
    28561 gcaaggcggg ggtgggggac gtccgggtac aggccgacta tggcgcgctg cccttcgcgg
    28621 ccaacagcct cgatctggtg gtgctgcccc acgtgctgga atttgcggcc catccgcacc
    28681 aggtgttgcg ggaggtggag cgggtgctgg tggcggacgg tcaggtggtc atcaccggct
    28741 tcaaccccta cagcctgttc ggcctgcggc ggctggctgc cggggaatgc atgggctatc
    28801 cgtggcatgg ccagtacctc agccagcacc gcctgaagga ctggctggcg ctgctgggct
    28861 tcgagatgcg cctgtgcgac gctggctgct acattcccgg cgtggagcag gaacaatgga
    28921 tcgagcgcct gcgcctcctc gaccgggcgg gccgccgctg gtggcccttc atgggcggtg
    28981 tgtacatggt gcaggcgatc aagcgggtgc agggcatgcg cctcatcatg ccccgctggg
    29041 accgcaaggc gggcgcccgg gcgctggccc cgctggtaca gaaaagcacg gccccacgcg
    29101 cgggctgtgc ccaacagaac aggaatgacg tttattttgg acgagattga catctacacg
    29161 gacggggcct gctcgggcaa tccggggccc ggcggctggg gcgcgatcct gcgcagtggc
    29221 gcccacgaga aggaactgtg gggcggcgag cccgccacca ccaacaatcg catggagctg
    29281 ctcgccgtga tccgcgccct ggaagccctc aaacggcccg tcgcggcccg cgtgcacacg
    29341 gattcccagt atgtgcagaa gggcatttcc gagtggatcc acggctggaa ggcgcgaggc
    29401 tggaagactg cctccaaggc gccggtcaag aacgaggatc tgtggcgcgc ccttgacgcc
    29461 caggccgccc gacaccaggt caagtggctg tgggtcaagg gccacgcggg gcatgtcgac
    29521 aacgaacggg ccgacgccct ggcgcggcgc ggcgtggagg cggtccgcaa ggcaggcgct
    29581 gcagtggagg gcgcagcatg agacagatcg tcctggatac ggaaaccacc ggcctggact
    29641 ggaagggcgg tgaccgggtc atcgagatcg gctgtgtcga gattgtggac cgcaagctca
    29701 ccggccggca ctaccacgtg ttcatcaacc cggagcggga catcgacgcg ggcgccatcg
    29761 cggtgcacgg catcaccaac gagttcctgg tggacaagcc cctgttccgg cagatcgtcg
    29821 aggagttcga ggccttcgtg gcgggcggcg aactggtcat ccacaacgcc agcttcgacg
    29881 tgggcttcct caacaacgag ctgggcctgc tcggccggcc ggcgctgacg gccatctgcc
    29941 cgagtgtgat cgacaccctc aagctcgcca aggaacagaa tcccggcaag aaggcgtccc
    30001 tcaacgcctt gtgcaccctc tacgagatcg acaacaccca ccgcagcctg cacggcgcat
    30061 tgctggatgc ggagctgctg gcggacgtct acctggccat gacccgcggc caggagagcc
    30121 tgatcatggc tctggatgaa ccgccgccgc ggctcgacga tggcagcggc ggtgcggcgg
    30181 ccagcgtgtg gccgcccctg cgggtgatcc ttgcctccgc ggccgaacag gcggcccacg
    30241 acgcggtgct cgccgagatc gccaaggcca acaaggggca ctgtctgtgg atcccgccgg
    30301 cgccgccggc cgagtcctga gtcagcccag cgccgcggcc accgcgtcgg ccagggccag
    30361 gcagcaggtc agtcccggcg attcgatgcc gaagagctgg accagacccg cgataccgtg
    30421 ttccgccggg ccgagcaggc ggaaatcggc ggcaggctcg ttcgggccgt ggatcttcgg
    30481 gcgcacgccg gcatgggccg gcaccagcgc cccgtccggc agcgccggcc agtagcgccg
    30541 cacggccggg tagaaggctg ctgcgcgatc ggaatcgacg cggtagtccg gcgcggcgat
    30601 ccactccacg tcgggcccga agcgcgcctg accgcccaga tccagggtca gatgcacgcc
    30661 cagaccggcg gcttccggga tggggtagat caggtgacgg aagggactgc ggccttgcag
    30721 ggcgaagtaa ttgcccttgg cgtaacgggc gacgggcacg caccgcgccg gaaacccctc
    30781 cagtgccccc gccagcctga tggcgccgag cccggcggcg ttgattacgc tgcgggcgcg
    30841 cagggtcatg ggcgccgtgc cgccggtgta gagctcgatg ccggcatccg agcagcgtcc
    30901 gcccagcacc ggcacgtggg tgaccagcgt cgtgcccgcg gcttcggcat ccgccagcag
    30961 ggccagcatc agggcatggc tgtccacgat gccggtgtcc ggcgacagca gggcgccggt
    31021 gcaggtgagg gcgggctcca gggcgcaggc ctcggtggcg gacagacggc gcagcccttg
    31081 cacaccgttg gcaatggcac acgccgcaat ggcgtcgagc tgcggcagct gtgcgtcgtc
    31141 cgtcgccacc agcagcttgc cgaggcggcg gtggggaatc gcccgcgccg cgcagtaggt
    31201 gtacagggcg tcgcggccgg gcacgcacag gcgcgcgcgc agggagcccg gcgggtaata
    31261 caggccggcg tggatgacct ccgacgagcg gctcgagatg ccactgccga agcgcgcctc
    31321 cgattcgagg atcagcacct cgcgacccgc ctgggccaag cgccgcgcgc aggccaggcc
    31381 gaccacgccg gcgccgacga cgacgcagtc gaccgtctcc atcacacgct caggcgcccg
    31441 tccggcaggc cgccggccgc gtcgaggcgc ttgagggtcg ggtgcaggtc gaggcccgta
    31501 tcggccttga gggcggcggc gcgggcgagc agcgcgtcgt gggacaactc gatgggcgca
    31561 ccgcgcaccg ccagggcgat ggcgttgccg gtctcggacg gtggcagcat gcacaggcgc
    31621 ccgtcgaagg cttcgcgaat agcgtcctgg cggcccttga aaccgcgctg gccgctgaac
    31681 aggttgaccg ccatcacgcc ccgttcgctc aggcgcgtcc agcaggcctg gtagaagggc
    31741 agggtggcga gatcgccggc gcgggcgtgg cggtcgaagc cgtccacggc gatgaggtcc
    31801 cagtgggcgt cggtgtccat cacgtattgg gcgccatcgc cgatcaccac ccggaagcgc
    31861 gcattttcat ctggcaggcg gaagaactgg cgggccacgg catgcacttc aggagcgatc
    31921 tcgaccacgg tgcacaccgc gtcgggcagg tgatgatgga cgaagcgcgc cagggagccg
    31981 gcgcccaggc cgatcagcag caccttgcgc ggccacggcc ggggatggag cagcaggccg
    32041 gccatcatct cgcgggtata gaccagctcc agcgaccagg gcttgcgcag gcgcatggcg
    32101 ccctggaccc attccgagcc gaaatggagg aagcggacgc ccgcttcttc actgatgtcg
    32161 atgggtacac tcatgttgga agaggtgtat gctgcaatgc aaaattccgg ggccggcaga
    32221 ctgcgcggtt ccgaacccga caggacagga gtacagcatg gtcagctttg aaaactacga
    32281 cagcgaactg gaatccatcg acatggagct ggcgcgtctg gcccagctct gtggcatcga
    32341 cctgcgtcgc cccggtgtgg ccgagcaggt gctgaagggc gatgccagcc tgtgcacgac
    32401 gtccaacccc atcgcctggg agaagctgcg cgggctgctg gtgctgcact accacgtggt
    32461 ccaggaagag gcgtccgagg aaggcaatgc ggtggcggcc gacagcgtgc gccgtgcggt
    32521 ggagcgcgtc accgcccgca tgaagtccgg ccgtcagtaa agccatggct cagtcccgcc
    32581 gcggcgtgta ggggcgggac tggaagcgga acagggggcg ttcgccgggc gccaccgcca
    32641 ccttcaccca gcccatgaag ggcgagccgt aggtttccag gcgggtgaag ttccggatcg
    32701 tctcgccgct cgccgggtcc ttgaggggct ggtcgatgcg gtgcagatgg gtatcgccgt
    32761 ggatcagcag cacctcgccg gcaaaggcgc gggtttcggc aagcaactgc ttcatcaggc
    32821 ggtcgaagcc gcggggatgg cggccggcgc cgaaatcctc gatgtccggg ttggcctgca
    32881 tggcgatcac cacgccctgg gcgccattcg cgcgggcgcg ggcaaaggat tcggcgagcc
    32941 aggcctgcac ggccgcgtcg cgcttgagga attcctcgct gggcgcgtcg ccatacccat
    33001 agttgttggc gctgccggtg acgttcaccg ccacgaagac caccgggccg atctgccagc
    33061 gctggttctc cacgtactcc ggaaagtctg ccgagtcggc ctgggtctcc agggccagcg
    33121 cggtctggcc gagggcgcgg tggccggcct tcgggaagaa catgccgcgc agtttgcgca
    33181 ggcgctccag gggatcaaag cggccgttgc tggcccggtg gcagtcggtc cattcgttgt
    33241 cgccgggcac gtagatcagg gcattgcggg aggtctggaa gagggcgagg cggtcgtcga
    33301 agaggacgtc gtcgcagcgc tcgccgccgt tcttgatgtc gccgtcgtgg atcacgaatt
    33361 ccaggggttc ggcgtccatc tcggccagca gattgggcag gtggaggcgc tcgaagcggt
    33421 tatagggcgt gtcgccgaag gcgccgaaga cgaagccgtc ggcagcatcg acgacgggtg
    33481 cggcggcaag ggcgaggccg gcgagggcgg aggcgagcag ggttttcatc gggtcaggcg
    33541 cttgagatcg tagagggctt cgagggcttc ccgcggggtc aggctgtccg gatcgatccc
    33601 cgccaaggcg gtgagggccg gatgggcagg tgcttcggct tccggctcgg gcagggcggc
    33661 gaacaggtcg gcctgggggc cggcgccgat ctcgcggttt tccagggcgc gcaggcggcg
    33721 tttggcgtcg cgcaccacgc tgccgggaat accggccagg gcggcgacct cgatgccgta
    33781 gctctggctc gccgggccgt cctccagggc gtgcaggaag acgatgccgt ggttgtgctc
    33841 cacggcgtcc aggtgcacgt tggcgcactc cgggtaatcc tgggacaggc gggtcagctc
    33901 gaagtaatgg gtggcgaaga gggtccagca gcggtttttg tccagcagat ggcgggcgat
    33961 ggccaccgcc agggcaacgc cgtcgaaggt ggaggtgccg cgcccgatct cgtccatcag
    34021 caccaggctg cgttcggtgg cgccgtgcag gatggcggcg gcctcggtca tttcgaccat
    34081 gaaggtggag cgcccggacg ccaggtcgtc cgaggcgcca atgcgcgtga agatcgcatc
    34141 caccgggccg atgcgcgccg ccgctgcggg cacgaagcag cccacgtggg cgagcagcac
    34201 gatcagcgcc acctggcgca tggtggtgga tttgccgccc atgttggggc cggtgatgag
    34261 gagcatgcgc cgggtggcgt ccaggcggca gtcgttggcg atgaagtttt ccacctggcg
    34321 ctccaccacc gggtggcggc cggcgcgaat gtccacgccc ggctgcgcgc tgaacaccgg
    34381 catggcgtag tcgtagcggg cggcggcttc ggcaaaggca cacaggccgt cgagcagggc
    34441 gcaggcgcgg gcgatgcgct gaaagacggg gatcgcctcg gcgaggctgt cgagaacggc
    34501 ttcgtagagc agcttctccc gcgccagggc gcgttcctgg gcggacaggg ccttgtcctc
    34561 gaaggccttg agctccggcg tgatgaagcg ctcggcgttc ttcagggtct ggcggcggcg
    34621 atagtcgtcg ggcagctcgc ccttggcggc gttggcggcg gagacctcga tgtagaagcc
    34681 gtgcacccgg ttgaattcca ccttgaggtt gggaatcccg gtgcgcgccc gctcgcgggc
    34741 ttccagttcc agcaggaagg cgccgcagtt gctctggatg ccgcgcagct cgtccaggtc
    34801 cgcatcgtag ccggcggcga tgacgccccc gtcccgcagc acgctggcgg gttcctcggc
    34861 gatggcgcgg gtgagcagat ccagcgcggc ctccggcgtg gcgagggcgt ccagcatctg
    34921 cgccagcagc ggcgcctggg gcaggcccaa ggcggcgcgc agttccggca ggcgcgccag
    34981 gctgtcgcgc agggacgaca gatcccgcgg ccgcgcgctc ttcagggcga cccgggcggt
    35041 gatgcgttcc acgtccgcca cgccccgcag cacgcggcgg gcgctctcgg cggcgccgtc
    35101 gaggagttcg cccacggcgg catggcgatg agccgcggcg tcccggtcgc acaggggatg
    35161 gtgcagggca tggcgcagcc agcgggagcc catgctggtg gcgcagctgt cgaggaggga
    35221 gagcagggtc ggcgccgcct cgccgcgcag ggtctcggtg agttccaggt tgcgccgggt
    35281 ggcggcgtcc aggcgcaggt aggcgctgtc ccgctctgcg gtgagggcgg tgatgtgggc
    35341 cagggcctgg cgttgggtgg cgcgggcgta ttcgaagagg gccgccgccg cggcaagggc
    35401 caccggcatc tcctccgcgc cgaagcccgc caggtcgcgg gtgccgaagt gctgggccag
    35461 cacgcgccgg gccgtctccg cgtcgaactg ccagtccgcc agccggcgca ggctgggcgc
    35521 cagggattcc agcaagggca ggcgcaggcc gtcggggatc agcacctcgg ccggacgcag
    35581 gcgctcgaac tgggccgcca gctgcggcgc gtcgcactcc agcacgcgga agtcgccatt
    35641 ggccagattg agccaggcca gccccagcac gccccggtgc agcgtgagcg ccagcagcgg
    35701 cgcatcggcc ttgtcatcca gcagcgccgc atcggtgagg gtacccggcg tgacgatgcg
    35761 cgacaccgcc cgctccatgg gccccttggt ggcgccgggg tcgcccacct gctcggcgat
    35821 caccaccgat tcgcccagct tcaccagccg cgccaggtac tgctccaccg cgtgatgcgg
    35881 cacccccgcc atcttgatcg gctgcccggc cgaggccccg cgggtggtca gggtgatgtc
    35941 catcagccgc gccgccttct ccgcgtcctc gaagaacagc tcgtagaagt cgcccatgcg
    36001 gtagaacagc agggtgtccg ggtaggcctg cttgatgcgc aggtactgct gcatcatcgg
    36061 ggtgtgagcg gaaatgtcag gcgcgggcgt gtcgtgcttc atggacggat ggcgagggga
    36121 atgcgggggc cgcggtgacg gctgccgcgg cgaggggcgt attgtcgggg aagtggacga
    36181 ggaagtgcac gtgcaatcgg cggtcgacct tgaattggcg ttgcgcagcc cgatctggag
    36241 ggtgaggaat gcgcgcaccg cgatgggttg tcgccgtgtt ccggggctgc tttactccta
    36301 caataccgcc cgacctctcc cctgcctgaa catcatgcct gccaattccg cgctgcccgc
    36361 tattacccgc ctgtatcagg cctgtgagcc tcaggaatcc ctcgcgcctt ccgacccgcg
    36421 ctgggtggat ttcgatccgg ttcgcggcga ggacaacgtg gtcgaacagt gcgcccgcag
    36481 tctgcgccgg gccgatccgg ccaaacccta ttttggtctg ttctccggtc atcgcggcgt
    36541 gggcaagacg agcgaacttc agcgcctcaa ggcccagctt gaagccggcg ccaacggatc
    36601 caagggcttc ctggtcgttt ttggcgatgt gagtgacgcc ctcgacgtca acgatctgga
    36661 tttccccgat ctgctggtct tcatcgccgc gcatctgcaa aaggagctcg ctgccaaggg
    36721 cctgcgcggc ttcaatccga tcaccacgta tctgacgaag acctgggacg acatctgtgc
    36781 actcctcacc gccgaggtgg aactcaagaa tgttcgcgcc gacacgggct tcggcaagct
    36841 cacgatggaa ttgcgcaacc gtcccaattc ccgcagccag ttgcgggatg ccgtggaaaa
    36901 gcacagcaca tcgctgctcc aggccgtgaa cgacctgctc gattcggccc aggcggcagc
    36961 actatccgcg ggttttgccg gcatggtcct catcatcgac ggactcgaca aactggtgcg
    37021 gcgcgggctc ggcgagggca gcaataccca tgatcgcctc ttcattgacc gctgcgatca
    37081 gttggcctcc ctgaaggtgc ataccatcta caccgtgccc atttccctga tctactctcc
    37141 ctttttcgcg caagtggaag aaacctttgg caaatgcaac gcgccggtgt cgatgatccg
    37201 actgcactcc cgcttcgatg agcccgtaaa gccggattcc gagggcatgc tcaagatgcg
    37261 ggagatggtg gacaagcggt gcagctacgc cgggatcgcg ccggaccagg tcttcgatgc
    37321 gccagccacc ctggaatatc tctgcgaaat gagtgggggc cacccccgcc acctgatgat
    37381 gtttgtccag gccgcctgta acgagctgga cgatctgccc atcacccgtc acgccgccgg
    37441 aaaagccgtc cgccgctacg ccaacgcgct gttacgggaa gtgcccgatg ccgcctggga
    37501 tgccctggcc cacttcaaga ccccccagcg agacatcccc aaggacgacc tgcaccagca
    37561 aatgatgttc ctgctgttcg tgttcgagta catgaacggt gccccgtggt acgaactcaa
    37621 cccggtcctg cgcacccttg agcgtcttgc ccgctgattc ccccctcgaa gcggcgggga
    37681 atgccttccg cgcgctgcgc cgcagcctct tgcgcagccg cggctttgcc gtgctggtgt
    37741 gcgtctgcga ttccctgagc aaccgcgatg agctcatcgc cgatctggcg gcgtcgctgc
    37801 cggccgtcac gctgcatcgc gtcgatgcag gcgacggcga ttgcgatctg ctggcgcgta
    37861 tcgtccagga gtttgccgac gctccgcccg gcccggtgat gatccttggg ctggaaagag
    37921 tactcgcgga tgcgcaggcg gccgagcgca tgctcgccgc cctcaacctg agccgggcgg
    37981 aatggccaac gcgcatggcc cagcccgtcg tgttctggtt gccccggcga tacctcgggc
    38041 gtctcaccgc cggcgcgccg gacttcttcg attggcgcag cgacacgctg gacttccccg
    38101 agctgagcgc tgtccagctc cggcctttcg gtcagcggga gtggaccttc gggggcgatc
    38161 cgcgcctgtc ccgcgccgag cgggaggagc gtattcggga gctacgggcg cgcatatcgg
    38221 ccct
//
LOCUS       DAOQPA010000020        37999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_439_length_37999_cov_18.804501, whole genome shotgun sequence.
ACCESSION   DAOQPA010000020 DAOQPA010000000
VERSION     DAOQPA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 37999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37999
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_37999_cov_18.804501"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKX01_04645"
     CDS             complement(<1..906)
                     /locus_tag="PKX01_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75444.1"
                     /translation="MTDAPRSPRSLRLFLSSPGDVADERQAVRRVLEDLALTPLLRTR
                     VRFDLVAWDDPAAPVPLDATETPQLSVNRYKGLPRDCDLTVVILWSRLGTPLPSHVTR
                     PDGSAYASGTVWELEDARTGRKPLWLYQRTATPQVDLDDPEFDAKRAQYHAVKAFIAD
                     FRAPDGSLSAGINTHADPAAFTILFRQHLEAHLGGLLFDAPPVAPDSPRIDHILLALD
                     AQLAAKDSTIAERDGEIARLKAELDALRRSAIADTLTRAAASDATPAEQAAAQALEAG
                     DSAPAEALLRADETALTPADRLPSSA"
     gene            1104..2726
                     /locus_tag="PKX01_04650"
     CDS             1104..2726
                     /locus_tag="PKX01_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75445.1"
                     /translation="MNLNMQKIWVRLVIVIASLMALGSAGVLWWVAREQQHTAIEQAE
                     EFAQSVHQMTMAQLMFAKATRNYKRQGYYLEQVEQSRGLRHLKVLRGAATVQQFGERE
                     QGVIPADDPLEKAVLADGKARYEIREEEGQPVLKAVLPAIAEKRYLGKECLECHDEAK
                     EGDVLGAVSMSVQLDAVKRNLRESLLTTVAVALLLGVVLVAAVYVFVRNALARPLNGM
                     TRNLADIAGGEGDLTRRLKARRQDEVGLASAAFNRMMDSLQRLIGQVIRSSGDVAGAA
                     SALERGTARIADGSTEQARRSASAAAGMEEIVGSIAQVADLSTRVQALATHSMTCTHH
                     GNETLTELDDKLHGIDRAVQEIVGTVENFVNRAESITAMTQQVKGISDQTNLLALNAA
                     IEAARAGESGRGFAVVADEVRKLAEQSHRSAAAIDTITGALGEDSSAARTAIQHGLAV
                     LRSSRESMRRVAEALGEANRAVEDAVRGTAEISAATGVQRDVSGRVAADVEGIAQLAE
                     RNGAEIADAAVAARQLAGLAAELQAETGRFRV"
     gene            complement(2768..3325)
                     /locus_tag="PKX01_04655"
     CDS             complement(2768..3325)
                     /locus_tag="PKX01_04655"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HMZ75446.1"
                     /translation="MTLDLDDIRRVRAEADCLCTPAEVDAALDRLAAAITERFHDRNP
                     LLYVVMNGGLVLAGRLLHRLDFPLEVAYLHATRYGHALQGTLLDWRVRPTQDLRDRHV
                     LVLDDILDEGHTLAAIIDYLKSEGARDVQSAVLVHKLHDRKAYPGMRADFTGLDVADR
                     FLFGCGMDYKGYWRNAAGIYALKGH"
     gene            complement(3337..3621)
                     /locus_tag="PKX01_04660"
     CDS             complement(3337..3621)
                     /locus_tag="PKX01_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HMZ75447.1"
                     /translation="MSPELVELALRKQRLQIRSAAQRQALAGHLAAFAPLLRGADAVT
                     DGLHWARRHPALLAGAAAFLLVTRPRRAVRWLRRGWFGWQLVRRARALVS"
     gene            complement(3630..4013)
                     /locus_tag="PKX01_04665"
     CDS             complement(3630..4013)
                     /locus_tag="PKX01_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMZ75448.1"
                     /translation="MSDSAPPRLGESLRGLADTGLGVLQTRLELFAVELKEEKLRVGS
                     FLFDTVLAALFIGFGIVFLLAFLTVLMWDEHRLLALGLATAGLFAAGIYAATRAATRL
                     RAGSRLFAASLAELAQDRDALRPED"
     gene            complement(4022..4330)
                     /locus_tag="PKX01_04670"
     CDS             complement(4022..4330)
                     /locus_tag="PKX01_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HMZ75449.1"
                     /translation="MSETQVTKDKLVADFKAVVADTEELLKLTAGTAGDKVADVRARL
                     TDKLGTAKEKLQDLEAAVVAKTKAAARATDDYVHDNPWKAVGVAAGVGFLLGLLVNRR
                     "
     gene            complement(4492..6141)
                     /locus_tag="PKX01_04675"
     CDS             complement(4492..6141)
                     /locus_tag="PKX01_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ75450.1"
                     /translation="MVPPFTRPVLRLLILWAGLLTALAALPAFAAPASSHPQKVQSAP
                     PDLPPAADSAPVLLGGEEVFRIAAPFKAYSPAVRARQISRRLADLATRGTAPEIRVEE
                     SDISSDLLADDQVLLSVFDADAAAEGIPRADLAARHAATLGAALARYHDEHRHEALLI
                     GLGKSAGAVLAALALLTLFERGYRRLREAILRKADETIASVERRSFSLVRGSYLRTPL
                     LALLHGIRLAVWASLLYAALHLSLASFPQTRRFATHLSELVLTPLGTLGHGLLDAIPG
                     LLVVVIIGVLARWLIRSASFIFERIGDGRIVLDGFYPEWAAPTRRIVHLLLIAGAVMI
                     AYPYIPGSDSAAFKGVSIFLGVLLSLGSSGVISNIVTGFMITYMRAFKVGDVVRIGET
                     TGIVVSSSLLVTRLRTIRNIEITVPNSQILNGQVTNFSVTGNPTVSTQVTIGYDTPWR
                     QVHALLLDAAARTTGIVHQPPPFVLQTALEDFYIRYELMCVVDNPTRMPFILSALHQA
                     ILDAFNEYGVQIMSPHFIDNPGNPAVVPREQWHAAPARPPA"
     gene            complement(6225..7379)
                     /locus_tag="PKX01_04680"
     CDS             complement(6225..7379)
                     /locus_tag="PKX01_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ75451.1"
                     /translation="MTAPLLILGTGLAGYTLAREVRKLDRDRPLVLVSRDDAPFYSKP
                     MLSNALAAGKSATTLVMKSAERMAEELGATILPRRRVASLDPAARHVQLDGGEPLAYG
                     DLVLALGADPIRLPLAGDGADDVLSVNDLDDFARFSARLDGVQRVVILGAGLIGCEFA
                     NDLLARGISPSVFDVAPWPLGRLLPDAAGRHFQARLEAAGVRFHLGQAAQAVHRDGPG
                     YRVELGDGRQEPADLVLSAVGLRPRTALATAAGLTVGRGITVDRRLATSDPHIFALGD
                     CAEVDGLHLPFVLPLMQQARALARSLTGTPTDVSYPPMPVLVKTPAIPTIVAPPPIGA
                     AGVWRIVEDTPDAGLEARFEAPDGSLRGFALLGPATARKQTLSAALPALL"
     gene            complement(7431..7595)
                     /locus_tag="PKX01_04685"
     CDS             complement(7431..7595)
                     /locus_tag="PKX01_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMZ75452.1"
                     /translation="MKTWQCIVCGFIYDETAGLPEEGIAPGTAWADIPDDWACPDCGV
                     AKSDFEMIEL"
     gene            complement(7630..8115)
                     /locus_tag="PKX01_04690"
     CDS             complement(7630..8115)
                     /locus_tag="PKX01_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HMZ75453.1"
                     /translation="MANPFHDPASATVQNLEAAFAGESMAHIKYRYFAKLCREMGDEE
                     TARAFEATADQEVMHAFGHLDLLYPKAKMSPARALEFAIEGETYEYTEMYPAFRHTAV
                     AEGHSAAVQEIDEQIAESREHAEQFKAVLEKAAKRFAALAKVEERHAAHYQARLDQID
                     A"
     gene            complement(8243..9031)
                     /locus_tag="PKX01_04695"
     CDS             complement(8243..9031)
                     /locus_tag="PKX01_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ75454.1"
                     /translation="MLRVVTANLNGIRSANVKGFLPWLALQQADVVCLQELKAQSADL
                     PPETVTPNGFRGFFSFAEKKGYSGVGIYAKKIPDQVIYGFGIPEFDAEGRYIQADFGN
                     VSIISLYLPSGSSSDERQAAKFRFMAHFLPHLAQLRCSGREVIVCGDWNIAHQAIDLK
                     NWRSNQKNSGFLPEERAWLSRLFDEQGWVDTYRRLHPDATDACYTWWSNRGQAWAKNV
                     GWRLDYQIATPGIAAQVRRCAIYKEQRFSDHAPLTVDYDHPFGD"
     gene            9070..9717
                     /gene="pyrE"
                     /locus_tag="PKX01_04700"
     CDS             9070..9717
                     /gene="pyrE"
                     /locus_tag="PKX01_04700"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMZ75455.1"
                     /translation="MDFSRDFIQLACDKGVLRFGEFVTKAGRLSPYFFNAGLFNDGES
                     FGRLCDFYARTLIAAHLPCDMLFGPAYKGIPLVAGTAMRMAERGVNLPFCFNRKEAKD
                     HGEGGTLVGAPLAGRVAILDDVISAGTSVRESVEIIRAHGATPAAVVIALDRMERGTG
                     SLSAVQEVQRTYDIPVLAVANLADLIAYLKDSPALAENLGAVTRYRETYGVAREA"
     gene            9724..10359
                     /locus_tag="PKX01_04705"
     CDS             9724..10359
                     /locus_tag="PKX01_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75456.1"
                     /translation="MMRRLLFCLLAAVSVPAGAQSSAKAPRVFCCPDASGHQICGDVL
                     PEQCYGKSYREMSPQGGVRRLVEAPLTPEQLARREAEERARKAEQARQRAETRRNQSL
                     LETYSSVQDIDNRRDKAIEAVQSELKQAEARYAQLQARRQGLTREAEFYQKRSMPVAL
                     ANSLRESDSELAAQWSVIEVKRRDIENIRARYEQDRARYIALTDPRAPDIR"
     gene            10748..11389
                     /locus_tag="PKX01_04710"
     CDS             10748..11389
                     /locus_tag="PKX01_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ75457.1"
                     /translation="MLQYKIVPVTPFEQNCSILWCDASRQAAVVDPGGNVDRILAAVQ
                     ALGVTVERILLTHGHIDHAGGTADLARQLAVPIEGPDREDAFWIEGLPQQSRMFGFPP
                     VGAFTPDRWLADGDTVQVGEETLDVIHCPGHTPGHVVFFSPSARLAVVGDVLFAGSIG
                     RTDFPRGNHAALIASIRTRLWPLGKDVAFIPGHGPMSTFGDERDANPYVGDYA"
     gene            11467..12210
                     /locus_tag="PKX01_04715"
     CDS             11467..12210
                     /locus_tag="PKX01_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75458.1"
                     /translation="MRFAILEDDPAQMEVLTGWLREAGHDVHGFAMARELMRVASRES
                     FDLFLVDWVLPDLTGEQVLRWLRTERNNDTPAIFITSRDAEDDIASAFSAGADDYLVK
                     PPRRIELLSRIEAVLRRTQPRQVSQLLQVAPYSFDLIRKMVTVNGEPVEMTDKEFELA
                     AFLFRNMGRLLSRGHLLEAVWGRNPDLATRTVDTHISRVRSKLNLRPENGFRLTPTYN
                     FGYRLERVDAANLAPDGAPAEGADLSSQV"
     gene            complement(12260..12679)
                     /gene="mscL"
                     /locus_tag="PKX01_04720"
     CDS             complement(12260..12679)
                     /gene="mscL"
                     /locus_tag="PKX01_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMZ75459.1"
                     /translation="MSIIQEFKEFALKGNVVDLAVGVIIGGAFQKIVDSLVKDLLMPV
                     LGKLLGGVDFKHLYINLGDKTYETLDAAEKAGAPLFKYGVFINTVIDFVIVAAAIFVA
                     IKAMNKLKREEPVPAPEAPVEAEDVKLLREIRDALKK"
     gene            12899..14488
                     /locus_tag="PKX01_04725"
     CDS             12899..14488
                     /locus_tag="PKX01_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ75460.1"
                     /translation="MALHRHRILSAVLGVLAWSGGAFAADEDIVYTARPGDTLIGLQK
                     QLLAAPFGWKGVMLHNRIAEPRRIPTGTQVRIPEAWLRVEARSAQVVGVHGEATLNGR
                     PLALEDQVPPGAVLRTGNASFVTLRMPDESRLTLQPDSEARLEKIQGFAGLTGQTAQI
                     HLERGRIENTVAAQRGPAARYRIRTPSASVSVRGTEFRVGSDGTDGRARAEVTGGEVR
                     VGAETGPATAVAMGFGVTAEPGGGVQPPQALLPAPALEGLATVHERTAVEITLRPVDK
                     AVAYRAQLARDAAFTDVVTEAVSPAPPVRLENVPDGRLWVRVRAIDAAGLEGFDTQRA
                     LEVRARPVPPDAAGPLAGAAFVGGPVHFEWTPVADAPGYRFQVLRRAAPEVLLIDREV
                     VTSGIDLTLPRGEFRWRVATLKPAGGQGPWGDARALAVTRPIGVPTVRRYNDRLRFEW
                     SGDDGQIYDFQLARDAGFGDLVVNQRLGEAAITVPVPAGGRYFVRVRGTDAQGAASPY
                     TPVQVLYRLPLLMWTLSNAAP"
     gene            14485..16815
                     /locus_tag="PKX01_04730"
     CDS             14485..16815
                     /locus_tag="PKX01_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 and HATPase_c domain-containing protein"
                     /protein_id="HMZ75461.1"
                     /translation="MKSGTGTVLRRPTLEWLAVTTLCIVLAVFAALEGWLWRIDQTIY
                     DSFLSSFRRATSANVAIVAIDDASLAELGSWPWSRRVHAALLDRLHDAGARAVALDLI
                     LDTPSRDDPAADTVLAQSMRAFGRVALPLTQATTGVLLTGEILPLPEFVAAAAALGHG
                     QVEFDPDGIARSVYLWEGFGSARHPQLALALLRIVDPEVAARYAQPGPGDDDDRLQWV
                     RATWLRVPFAGPPGTYPYYSYAAVLRGEVPADALRDKVLLVGATASGMAEAVPTPMSG
                     LSRPMPGVEVDANVYDALRRGDAVRPLSAGTSAGVAALAVLVAMLALFYGDPRTGLLV
                     SVLAGLAALALAAVALQAGSMWFGPAPAVLGCMLAYPLWSWRRLEWAQRYLDAELAAL
                     EREGDALPSEVDRGAADRLERRIRIVRHVARQQRDVRRFIADTLEHLPIGVVVIDPGN
                     RVVLHNERARHLLQAFRGEDLLAALRSLPWPVYLPMRDGLPDLAGFAGIRQFELPVGR
                     RHLLLSVAELVAGTGSLSGRVIGLADVTVMHEAQRTREDTMHFLSHDLRAPLASILTL
                     IDGYKGDPAAQAEQIPRIARYARSALDLADGLFRLVRAEGSDPKDFVELDLAGLVDSA
                     ADEVWPQAHARGIRVAVDTRAAETEEPIVRGDFSLLRRAVINLLTNAIKYGSPDSTVS
                     AELAAEGADWVIRVRGQGAGIPPDALPRLFKRFSRIVEEGRDRPSGVGLGLLIVKTVA
                     ERHGGNVSVESTPGVGSMFEIRLPLRMRPDAPSRPD"
     gene            16856..19000
                     /locus_tag="PKX01_04735"
     CDS             16856..19000
                     /locus_tag="PKX01_04735"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HMZ75462.1"
                     /translation="MGFKPLKLLAFLLGGLSAVMGAGALYLYATFDGARLAAELTQSL
                     RQQTQRTVRLDGPVALSLFPRLSLRLPATTVSDPRSDGAFLAFERAELRVGLWPLLSR
                     RVAIEAVELQGPQLTLRRRRDGAVNTDDLAALLQGQEGDTALPLTADVHRLVVHDGRV
                     DWLDEAGGRRLGVTGLTLETDDLASQSVGRLSLTGHLRGDSPNVEVDLRVASDYRLDA
                     VGGRHALRHLSLSAVGVLAGRGDLEARASLDELAWVTGGELVVKGAAGDLRARAAEGA
                     PVLHLATPDLRLTANGPVAATVDADARWGSEAEGGRLTARLSDLQARAAGISVTADLR
                     TGDDRLNLQGRGAGDWRAAESLAQLRGVVLEGTWTRRGGASSPVRAGADLQVDTARGS
                     AGGTVDLRIDGSRINGTWALSRFAPWAGRFELEADRLDADRLFAATGDRWDLSGLHGF
                     DVDGQLRIGSLRAAGLQFERVRLPLSLHGGRLVSRGHAAGLYGGAVAGNFTLVADGNK
                     AFYTGQINGVSVEPLLRDATGREALSGTLNAFVDVATAAGRRSALAGALAGKGRFVLG
                     HGAVRGIDPFAALREWQAAIAARRGARRTFRDAEQAGLTEWSGSFGIERGVVSSADLQ
                     ARSAQWRASGHGVLDLAAGRIDVQQRLTVLALPPGAEGIALASLRGATLPVRVTGPLG
                     RAEWLLEPSSGVQSAPPGGPGAGRGPADGGRR"
     gene            complement(19022..20365)
                     /locus_tag="PKX01_04740"
     CDS             complement(19022..20365)
                     /locus_tag="PKX01_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ75463.1"
                     /translation="MSSTPALLDALDVGIILVDRDGRVQHWNRWMVTFSGLPARDILG
                     SRLSTAFGTLMDPRIAVAIREAIDFGRSSHLSQVFHPAPLPLHNPADPGAPRLRQMID
                     ASPVDDGDGRSCLLQVRDVTETVRREALLRSQARRLQEDLARLSAAQAELQRSELRFR
                     ELARQAPAGLFETDAQGRLIFLNERCAQMLGRSPADLPDEPWTHLLPPDDVDRMTARW
                     RRAVQTGNRFSEQFRYRHPNGSDLWIRAEAGVVREGSGIGGFIGTMVDVTEFHEQVVR
                     NEFRANHDSLTRLLNRERFDVLVRAAVSGAREVGQKIAILFIDLDQFKAVNDTHGHAA
                     GDRVLKSVAGRVRRVVRGEDVVARYGGDEFAILLHDAGDEAMLKRLTANIERSIALPI
                     PVGTGQVQIGCSIGVAIYPDHGRDPLTLLAHADRQMYRIKRSRWEAAEPDRDPVA"
     gene            complement(20373..21002)
                     /locus_tag="PKX01_04745"
     CDS             complement(20373..21002)
                     /locus_tag="PKX01_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HMZ75464.1"
                     /translation="MRPLSELECDALSEAFNLALGEAAATFASIVREEITLSAPVVEI
                     ITRDTLIARLQAAGCGNGTSRICSIDQRFHADMHFDSDALLLFPEQGSLEIVRRMIGD
                     ETPIEQITELEQDALAEIGNIIINSCMSSLANLFGTEMHGSLPVVRSCTADTLLGVRQ
                     ASDVILVAQINMRMSAHDISGYVLFIMDVPSIESFMRQVARLFNLPERV"
     gene            complement(20999..21373)
                     /locus_tag="PKX01_04750"
     CDS             complement(20999..21373)
                     /locus_tag="PKX01_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75465.1"
                     /translation="MTAAPVILLVDDSRVSRMLCGAIVRERRPDAILVEAGNGDEALS
                     CIATLVPHLAVLDVNMPGMNGLELAERIRNDHPDMRIALLTANVQDSVRQRAAALNAA
                     FFAKPINDTVVGRILDLLESHP"
     gene            21610..22209
                     /locus_tag="PKX01_04755"
     CDS             21610..22209
                     /locus_tag="PKX01_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HMZ75466.1"
                     /translation="MNFLAHAWLAGPHPADRLGGLMGDFIKGPLPGALAPAVASGVRL
                     HRRIDVFADSHPAFLRSRARVSTARRRVAGVMVDMFYDHFLARDWAEFHPEPLADFTA
                     AQYRLLEEAPGLPERLQKILPSMRDDDWLASYRDVETVAYALERMAGRLRPGNPLAGG
                     GAELLMHYAEFAADCREFLPDARAFAQAFWQRDGFSAAE"
     gene            complement(22193..22483)
                     /locus_tag="PKX01_04760"
     CDS             complement(22193..22483)
                     /locus_tag="PKX01_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75467.1"
                     /translation="MKRHPSLAAALLLTLGCAATVPAYAHGGGEGHHHGAWGWGPRHH
                     HYYPVREPVMVVPPQAYYAPPPVYYAAPPRPVYYTPQPSVVIGIPPLVIPLR"
     gene            complement(22687..24585)
                     /locus_tag="PKX01_04765"
     CDS             complement(22687..24585)
                     /locus_tag="PKX01_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75468.1"
                     /translation="MSAAHDAILLAGAEELLMLVDPATLAILAVNPAAERLLGLRAEA
                     LVGQPVTEIECALADLFFWEEVRSGGGADEQVMEGLYRRADGTLLSARKCVRRVPAGM
                     LVRATDIGAQKKAETELALMTAQLRATLEATADGILVIDADGRVVNMNRRLARQWQLP
                     ETLLAHRDDDALIAHMAAALESPAPFRSLLTDHLFDLTDTFDTFVFRDGRRCEITSRP
                     ARQAEQVIGRVFCFTDVTERHRSQQALIAARDAADAANRAKSDFLAIMSHEIRTPMNG
                     IIGMAQLLGMSEHRPEEREYIDTILTSSDALMGIINDILDYSKIEARKLTLERISFDL
                     HQLVTDVERMFRPKALEKGLQFGATLATTLPRVVMGDPTRLRQILVNLVSNAFKFTPQ
                     GAIRIDVEPAEALRGGRVIVRFAVTDTGIGIPADKCEHIFTPFEQADMSTTRRYGGTG
                     LGLAICRMLCTLMEGEIRVDSQVGAGSRFDFSVALDVGVAEERPTVETPRALLRRDTR
                     ILIAEDNPVNQVVLARLLSRLGATQPAFARDGREAVDLGTADRYDLIFMDARMPVMSG
                     IEATQALRERGCRAYIVGCSADTLPDDIRLALDAGMDDYLTKPVSHEALGAAIERWRR
                     HLGETPTG"
     gene            complement(24582..25865)
                     /locus_tag="PKX01_04770"
     CDS             complement(24582..25865)
                     /locus_tag="PKX01_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HMZ75469.1"
                     /translation="MPHTASPQDVFVTPEQLCVGLFIHVDLPWFSHPFSFNSFKIKSD
                     EQIATLRTLGVSRFRCDPARSDLPPAVGASEPRDAPAPPPEAPAADDPALAAKRERIL
                     RLQQRKRQVIEVEQAFVKAANVMRDIGKNLFARPKECLEEVDELVNQMVVAFLDQPEV
                     ALHVIGEHAGGEEAYYHGLNCSILAMMLAGELGLPRAAGQTLGVGALLHDIGLVDIPD
                     RIARARHELTPPERHLREMHCDYGVRLARQIGLPEPVLRIIAQHHEMADGSGYPKALK
                     GDQIDPCARLVALVNYYDNLCNPGDLAKALTPHEALSLMFAQRRGRFDAQALQVMIRC
                     LGVYPPGTVVKLSNDALALVASVNPEKPLRPWVTVYDPDIPKDEAIMLDLEQEPDINI
                     AKALRPIVLPPEVYEYLSPRKRVTYYFDANRRRGQ"
     gene            complement(26015..27022)
                     /locus_tag="PKX01_04775"
     CDS             complement(26015..27022)
                     /locus_tag="PKX01_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMZ75470.1"
                     /translation="MSVRRLALALSLCGATAAIAASPARPQEPITPLAPTKVSAPALV
                     ELGKKLFFDPRLSRSGFISCNSCHNLAMGGTDNLKTSIGHNWQEGPINAPTVLNSSLN
                     LAQFWDGRAPDLKAQAGGPIANPGEMASTHVLAVEVIQSIPGYSAEFKQVFGSDKVDI
                     DKITRAIAAFEETLFTPNSRFDQWLKGNKKALNADEVAGYTLFKDSGCVACHNGPAVG
                     GNSFQKMGVVAPYTASSPAEGRSAVTGKDADRFNFKVPTLRNVELTYPYFHDGAATTL
                     KDAVDTMGRIQLGKHFTDAENARIVAFLKTLTGAQPNFKLPILPPSSDATPRPEPFGR
                     K"
     gene            complement(27442..29277)
                     /locus_tag="PKX01_04780"
     CDS             complement(27442..29277)
                     /locus_tag="PKX01_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HMZ75471.1"
                     /translation="MSAPAALPPDALREHRLSLAEVTDLLVADRLVDRPDADDFLNGP
                     ARLRGEIHPLVLLAEQQWTARGAPSRPLDLETLTEWLAGRVSLDYFRIDPLKIDFTAV
                     TAVMSAAYAARFAILPVKVTPQEVVIATAQPFLREWEGELAGILKKRIHRVLANPLDI
                     ARYLVEFHNLARSVKQAAQGNHGGGVSSFEQLVELGQTNRQYDANDQHIVNIVDWLWQ
                     YAFEQRASDIHMEPRRDMGLVRFRIDGVLHQVYQMPMPVMNAMTSRIKILGRMDVVEK
                     RRPQDGRIKTRVASGKEVELRLSTLPTAFGEKLVMRIFDPQVLVRTLAELGFSDTDEG
                     RWHTITRRPHGIVLVTGPTGSGKTTTLYSTLKQLATPEVNVCTIEDPIEMVEPSFNQM
                     QVQHSIDLGFAEGVRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDA
                     PSAVTRMLDLGVPAYLLSATLLGIMAQRLVRILCPHCKTAAPLTPDEEAQWDHLVTPW
                     KAARPSRLHHPVGCMECRMTGYLGRIGLYEILLLSPELRRIVVGSGDLAQVRLQAIRE
                     GMKPLRISGALKVAAGLTTLEEVLKVAPPAEDGHERMPEDGSGRP"
     gene            complement(29402..29950)
                     /gene="ruvC"
                     /locus_tag="PKX01_04785"
     CDS             complement(29402..29950)
                     /gene="ruvC"
                     /locus_tag="PKX01_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMZ75472.1"
                     /translation="MSLTTERVATRILGIDPGLRITGFGVIDTLGSELRYVASGCIRS
                     GEGSLPQRLKVILDGVREVIATYRPDQAAVEIVFVNVNPQSTLMLGQARGAAICGAVS
                     CELPVAEYTALQVKQAVVGHGKAAKQQVSHMVERLLTLEASPSPDAADALACAICHAH
                     ASAGLAALAGVGPRKRGGRLTR"
     gene            complement(29947..30804)
                     /locus_tag="PKX01_04790"
     CDS             complement(29947..30804)
                     /locus_tag="PKX01_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ75473.1"
                     /translation="MTRLYPLLFVLLWSTGFIGAKFGLPYAEPLVFLCIRYTLVIALM
                     GAAALLARAPWPQDPRQWLHIGLTGVLVHGVYLGGVFTAIRHGLPAGVTALVVGLQPL
                     LTALGAGVLLGERVLPRQWAGLVLGFAGVGLVVAHKVAAAADAHGLTAMLVPALAALA
                     GITVGTLYQKRFCPAFDLRTGAVIQFVPSLALSALVAWHTESLTVAWTGSFVFALMWL
                     VLVLSVGAISLLNLLIRSGSAVNVASLFYLTPPTTALIAWAAFGETLTGLALAGMGLT
                     AFGVWLARR"
     gene            complement(30863..31096)
                     /locus_tag="PKX01_04795"
     CDS             complement(30863..31096)
                     /locus_tag="PKX01_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75474.1"
                     /translation="MSIPTERRKNLHLRDLVDKAYALIEPFFDPANAWNGQTLEHLAY
                     RVVRENLPGIPPEDVQVVVSAATRIYRAKIGPQ"
     gene            31211..31693
                     /locus_tag="PKX01_04800"
     CDS             31211..31693
                     /locus_tag="PKX01_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMZ75475.1"
                     /translation="MRFWLMKSEPDDVSIDDLATRPGQIVPWYGVRNYQARNFMRDAM
                     TVGDGVLFYHSGCAEPGIAGIARVASAAYPDATQFDPASRYFDPKATPEQPRWVHVDV
                     AFVRKTRLLGLSELRTHPELATLRVLARGNRLSITPVEPAEWRFIVGDLLGATDLKDG
                     "
     assembly_gap    31755..31854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31863..32654
                     /locus_tag="PKX01_04805"
     CDS             31863..32654
                     /locus_tag="PKX01_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ75476.1"
                     /translation="MAAYLLLGAFVGFFAGLLGVGGGAIMVPMLTTLFAAQGFPREHM
                     VHLALGTSMAAIVVTSISSLRAHHAHGAVRWEIVKGIAPGVLLGTFGGSFVASRAPTA
                     PLALFFASFTAYVAVQMILNKKPKPSRELPGPAGLAAVGGGIGFISSLVAIGGGSLSV
                     PFMTWCNVKMQHAIGTSAAIGLPIAVAGALGYLINGWGTEGLPAWTAGFVYLPALVLV
                     SAVSSLTAPIGARLTHRLPVATLKKVFAGVLILLSAKMLHTVFGG"
     gene            32739..33407
                     /locus_tag="PKX01_04810"
     CDS             32739..33407
                     /locus_tag="PKX01_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75477.1"
                     /translation="MSDATVRLQLHFELELATPPELAGLDHAALCRTLADALGPTVLR
                     GLPAIAGKQLTPLGARVVRHHHHLDAAAQAARRVERAQVIQVAPHLTDAEVDTLVQRA
                     AARLPAAESEQAAYLRGQALALVNEYRLVPCVVEAQLTSGKPAHIEGRLNLTNGHIFL
                     DASHRQTRLQAGQGALRVSIAGTPVALAAECSGHTLTGPVLDVAVASLVPHRDVLLAC
                     WQAG"
     gene            complement(33423..34205)
                     /locus_tag="PKX01_04815"
     CDS             complement(33423..34205)
                     /locus_tag="PKX01_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ75478.1"
                     /translation="MSGEIVLQRDGGIATLSLSNPDKLNAVDAGMWRALHATQTALNA
                     DPDLRCVLLRGAGDKAFAAGGDIEEFLTVRATVDDALHYHEDLVAAALDAIRACPVPT
                     VAMIQGACIGGGLEIAGCCDLRIAGESSRFGAPINKLGFSMYPGEMAGLLDLVGPAVL
                     LEILLEGRILSAREALAKGLLTRVVDDEEVEREALATVSRILKGAPLVARWHKQWVHR
                     LADRRPLGTDEKRAAFDFLATEDYREGLDAFFAKRTPDFKGR"
     gene            34444..34776
                     /locus_tag="PKX01_04820"
     CDS             34444..34776
                     /locus_tag="PKX01_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HMZ75479.1"
                     /translation="MSTASQSAVYARIRSNPRFAELVRKRQGFATILSVIVLTIFYGF
                     FMVVAFNPQLIGQRLSEGSYMTVGIAAELFMFIFFWILTAVYVKRANSEFDDITTAIV
                     NDAAKEVK"
     gene            34776..36440
                     /locus_tag="PKX01_04825"
     CDS             34776..36440
                     /locus_tag="PKX01_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HMZ75480.1"
                     /translation="MIARLNLALLGLLAPALACAGDAISATEKQPLNLHAIGMFVLFV
                     SMTLGITYWAAGRTKSAADFYTAGGGITGFQNGLAIAGDYMSAATLLGLTALVYTKGV
                     DGYIYMIAFFAGWPIILFLMAERLRNLGKFTFADITSYRLNQSKVRTMAAVSSLTVVC
                     FYLVAQMVGAGQLIKLLFGLDYTIALFVVGALMMVYVTFGGMVATTWVQIIKACMLLA
                     GGTLVMVLAMSQFGFSFENLMTQAVSLHSLGEKILFPGSLLPDPVTALSLGLGLMFGT
                     AGLPHILMRFFTVTNAKEARKSVLYASGFVAYFFNVILLMGFSAIIIVGQNPDFFEGG
                     KIGGKIIGGGNMVAMHLAKAVGGNLLLGFLSAVAFATILAVVSGLALAGAAAISHDIY
                     ARVIRGGKATEMEELRVSKLSSVALGIVAILLGIVFEKMNVAFMVALAFGIAASANFP
                     VLILSMYWRGLTTRGAVVGGYVGLVAAVLFVLLSKSVWVTVLGNAQAIFPYDQPALFS
                     MPLAFLVTIVLSKLDNSAEAQAERAAFEDQYVRAQTGFGAAEASQH"
     gene            complement(36540..37214)
                     /locus_tag="PKX01_04830"
     CDS             complement(36540..37214)
                     /locus_tag="PKX01_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMZ75481.1"
                     /translation="MNATRIAPLALYQEVAERLRQRIFAHELPPGTWVDEQALAAQYG
                     ISRTPLREALKVLASEGLVTLKPRRGCYVTEISERDLDEIFSVMALLEGQCAASTARR
                     AGDGDLVRLHTIHDELEKAAASNDIDGFFEANQAFHQELQRIANNRWLTQAIVDLRKV
                     IKLSRHHSLFIDGRLQQSLLEHRVILAALDRRDEAGAEAAMREHISSARRALARIAST
                     RDEAAA"
     gene            37491..>37999
                     /locus_tag="PKX01_04835"
     CDS             37491..>37999
                     /locus_tag="PKX01_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ75482.1"
                     /translation="MANDSIPTFPQPDLAAWNKAAAKSAPEGDVAKLNWVTPEGITVK
                     PLYTAADVNGLSFTDTLPGFEPFLRGPQATMYAVKPWTIRQYAGFSTAEASNAFYRKA
                     LAAGGQGVSVAFDLATHRGYDSDNPRVVGDVGKAGVAIDSVEDMKILFNGIPLDKISV
                     SMTMNGAVLP"
BASE COUNT         5629 a        11751 c        14238 g         6281 t
ORIGIN      
        1 ggcagaggag gggaggcggt cggcaggggt gagggcggtt tcgtcggcgc gcagcagggc
       61 ttcggcgggg gcgctgtcgc cggcttcgag ggcctgggcg gcagcctgtt ctgcgggggt
      121 ggcgtcggag gcggcggcgc gggtgagggt gtcggcgatg gcgctgcggc gcagggcgtc
      181 gagttcggcc ttcagacggg cgatttcgcc gtcccgctcg gcgatcgtgg agtctttggc
      241 ggcgagctgg gcgtcgaggg cgagcaggat gtggtcgatg cggggggaat ccggggcgac
      301 gggaggcgcg tcgaagagga gcccgcccag gtgggcctcc aggtgctggc ggaacaggat
      361 cgtgaaggcg gcagggtcgg cgtgggtgtt gatgccggcg gagaggctgc cgtctggcgc
      421 gcggaagtcg gcgatgaagg ctttgacggc gtggtactgg gcgcgtttgg cgtcgaactc
      481 cgggtcgtcc agatccacct ggggcgtggc ggtgcgctgg tagagccaca ggggcttgcg
      541 gccggtgcgg gcgtcttcca gttcccacac ggtgccggag gcgtaggcgc tgccgtccgg
      601 gcgggtgacg tgggacggca ggggggtgcc gaggcggctc cacaggatga cgacggtgag
      661 gtcgcagtcg cggggcaggc ccttgtagcg gttgacggag agctggggtg tttcggtggc
      721 atccagggga acgggggcgg cggggtcgtc ccaggcaacg aggtcgaagc ggacgcgggt
      781 gcgcagcagc ggggtgaggg ccaggtcttc gagaacgcgg cggacggcct gccgctcgtc
      841 ggcgacgtcg ccgggggagc tgaggaagag gcggaggctg cgcggcgagc ggggggcgtc
      901 ggtcatgacg gcgggtggac ggggggatga cgcgggcaag tataggccgg ccggcgcggg
      961 ggcggccagt cggggggcgg gcgtccggga ccggtccggg cagcaaaacc ccttgttgca
     1021 tctcaactat tgcctgcccg cgccgttagg cggctcggag accccgccga gacggggcgc
     1081 cgaccgtttt gtcgagggat ttcatgaacc tgaacatgca gaagatctgg gtccgcctgg
     1141 tgatcgtgat cgcgtcgctg atggcgctgg gatcggcggg ggtgttgtgg tgggtggcgc
     1201 gggaacagca gcacacggcc atcgagcagg cggaggagtt cgcccagtcg gtgcatcaga
     1261 tgacgatggc gcagttgatg ttcgccaagg cgacgcgcaa ctacaagcgc cagggctatt
     1321 acctggagca ggtggagcag tcccgcgggc tgcggcatct gaaggtgctg cgcggggcgg
     1381 cgacggtcca gcagttcggc gagcgggagc agggggtgat tccggcggac gatccgctgg
     1441 aaaaggcggt gctggccgat ggcaaggcgc gctacgagat ccgtgaggaa gagggccagc
     1501 cggtgctgaa ggcggtgttg ccggccatcg cggagaagcg ctacctgggc aaggagtgcc
     1561 tggaatgcca cgacgaggcg aaggagggcg acgtgctcgg ggcggtctcc atgagcgtgc
     1621 agctggatgc ggtgaagcgc aatctgcggg agtcgttgct gaccacggtg gcggtggcct
     1681 tgctgctggg ggtggtgctg gtggcggcgg tgtatgtctt tgtgcgcaat gcgctggcgc
     1741 gtccgctgaa cgggatgacc cgcaatctgg cggacatcgc cggcggcgag ggggatctga
     1801 cccggcgcct caaggcgcgg cgtcaggatg aggtggggct ggcgtcggcg gccttcaacc
     1861 ggatgatgga ttcgctgcag cgcctgatcg ggcaggtgat ccgctcgtcc ggcgacgtgg
     1921 ccggggcggc gtcggccctg gagcggggca cggcgcgcat cgcggacggc tccaccgagc
     1981 aggcgcggcg ttcggcgtcg gcggcggcgg gcatggaaga gatcgtgggc agcattgccc
     2041 aggtggcgga cctgagcacg cgggtgcagg ccctggcgac ccacagcatg acctgcaccc
     2101 accacggcaa cgagaccctg acggagctgg acgacaagct gcacggcatc gaccgggcgg
     2161 tgcaggagat cgtcggcacc gtggaaaact tcgtgaatcg ggcggagtcc atcaccgcca
     2221 tgacccagca ggtgaaggga atctcggacc agaccaatct gctggccctg aacgcggcca
     2281 tcgaggcggc gcgggcgggc gaatcgggcc gcggctttgc ggtggtggcg gacgaggtgc
     2341 gcaagctggc ggagcagtcg caccggtcgg cggcggccat cgacaccatc accggtgccc
     2401 tgggggagga ttccagcgcg gcgcggacgg cgatccagca cgggctggcg gtgctgcgtt
     2461 cgagccggga gtccatgcgc cgggtggccg aggccctggg cgaggcgaat cgggcggtgg
     2521 aggatgcggt gcgcggcacg gcggagatct cggcggccac gggtgtgcag cgcgacgtga
     2581 gcgggcgggt ggcggcggac gtggagggca tcgcccagct ggctgagcgc aatggcgcgg
     2641 agattgccga tgcggcggtg gcggcgcgcc agctcgccgg gctggcggcg gaactgcagg
     2701 cggagacggg acgcttccgg gtgtgagcgc ccggcgcggg gccgcggcgc tcggcctgcg
     2761 gtgcgcctca gtgtcccttg agggcgtaga tgccggcggc gttgcgccag tagcccttgt
     2821 agtccatgcc gcagccgaac aggaagcggt cggccacgtc caggccggtg aaatcggcgc
     2881 gcatgccggg gtaggccttg cggtcgtgca gcttgtgcac cagcaccgcg gactggacgt
     2941 cgcgggcgcc ttcggatttc aggtagtcga tgatggcggc gagggtgtgg ccttcgtcga
     3001 ggatgtcgtc gagcaccagc acgtgacggt cgcgcaggtc ctgggtgggg cgcacgcgcc
     3061 agtccagcag ggtgccctgc agggcgtggc cgtagcgggt ggcgtggaga taggcgacct
     3121 ccagcgggaa gtccaggcga tggagcagac gcccggcgag gaccaggccg ccgttcatga
     3181 ccacgtagag cagcgggttg cggtcgtgga agcgttcggt gatggcggcg gcgaggcggt
     3241 caagggcggc gtccacctcg gccggtgtgc acaggcagtc ggcttcggcg cggacgcggc
     3301 ggatgtcgtc gagatcgagg gtcatggttc ggaggttcag gagaccaggg cgcgggcgcg
     3361 gcgcacgagc tgccagccga accagccgcg gcgcagccag cgcacggcgc ggcgggggcg
     3421 ggtgacaagc agaaaggctg cggcgccggc gagcagggca gggtggcggc gcgcccagtg
     3481 cagaccgtcg gtcacggcgt cggccccccg cagcagcggt gcgaaggccg ctaggtgccc
     3541 ggcaagggcc tgacgctggg ccgcggaacg gatttgcagg cgctgcttgc gcagggcaag
     3601 ttcgaccagt tcggggctca tgcgtggggt cagtcctcgg gccgcaaagc gtcccggtcc
     3661 tgggccagct cggccagact ggcggcgaag agccgcgaac cggcacgcag acgggtggcc
     3721 gcgcgggtcg ccgcatagat gcccgccgcg aagagcccgg cggtggcgag gccgagcgcc
     3781 agcagacgat gctcgtccca catcagcacg gtgaggaagg cgagcaggaa gacgatgccg
     3841 aagccgatga agagggcggc cagcacggtg tcgaagagga agctgccgac gcgcagcttc
     3901 tcttccttca gctcgacggc gaagagctcg agccgggtct ggagcacgcc caggccggtg
     3961 tcggcgagcc ccctgaggga ctcgccgagg cgcggcggcg cggagtcgct catgcgccgg
     4021 ttcagcggcg gttgaccagc aggccgagca ggaagccgac cccggcggcg acgccaacgg
     4081 ccttccacgg gttgtcgtgc acgtagtcgt cggtggcgcg ggcggcggcc ttggtcttgg
     4141 caaccacggc ggcttcgaga tcctggagct tttccttggc ggtgccgagc ttgtcggtga
     4201 ggcgcgcgcg cacatctgcg accttgtcac cggcggtgcc ggcggtgagc ttgagcagct
     4261 cttcggtgtc ggcgacgacg gccttgaaat cggcaaccag tttgtccttg gtgacttggg
     4321 tttcgctcat ggtgttctcc gggcgtcttg tttcaggaag gaaggtgatg ggtggcgcgg
     4381 gacggtctgg gccgcctggg gcatcggttc taggctaatc ccaatcgatc gggaaggcaa
     4441 tttgaaggga ccggtaaaac gccggaccct tgatctggcg cagggtccgg ctcaggcagg
     4501 cggccgcgcc ggggcggcgt gccactgttc gcgtggcacc acggccgggt tgccagggtt
     4561 gtcgatgaaa tggggggaca tgatctgcac gccgtattcg ttgaaggcgt cgaggatggc
     4621 ctggtgcagg gcggagagga tgaagggcat gcgtgtcgga ttgtctacga cgcacatgag
     4681 ttcatagcgg atgtagaaat cttcgagggc ggtctgcagg acgaagggcg gcggctggtg
     4741 gacgatgccg gtcgtgcgcg cggcggcgtc gaggagcagg gcgtgcacct ggcgccaggg
     4801 cgtgtcgtag ccgatggtga cttgcgtcga gacggtggga ttgccggtga cgctgaagtt
     4861 ggtgacctga ccgttgagga tctgggaatt gggcacggtg atctcgatgt tgcggatggt
     4921 gcgcaggcgc gtgaccagca gggagctgct caccacgatg ccggtggtct cgccgatgcg
     4981 caccacgtcg cccaccttga aggcgcgcat gtaggtgatc atgaagccgg tgacgatgtt
     5041 ggagatgacg ccgctggagc ccagggacag gagcacgccg aggaagatgg agacgccctt
     5101 gaaggcggcg gagtcggagc cggggatgta ggggtaggcg atcatcaccg cgccggcgat
     5161 gaggagcaga tgcacgatgc gccgggtcgg tgcggcccat tccgggtaga agccgtcgag
     5221 gacgatgcgc ccgtcgccga tgcgctcgaa gatgaagctg gcgctgcgga tcagccagcg
     5281 ggcgagcacc ccgatgatga ccaccaccag gagcccggga atggcgtcga ggaggccgtg
     5341 gccgagggtg ccgagggggg tcaggaccag ctccgacaga tgggtggcga agcgtcgcgt
     5401 ctgggggaag gaggcgaggg acaggtgcag ggcggcgtac agcaggctgg cccacacggc
     5461 caggcggatg ccatggagca gagccagcag cggggtgcgc aggtagctgc cgcggaccag
     5521 gctgaagctc ctgcgctcga cgctggcgat ggtctcgtcg gccttgcgca ggatggcttc
     5581 acggaggcgc cggtagccac gctcgaagag ggtcagcagg gcgagggcgg cgagcacggc
     5641 cccggcgctt ttgccaaggc cgatgaggag ggcttcgtgg cggtgttcgt cgtgatagcg
     5701 ggcgagggct gcgccgaggg tggcggcgtg gcgggcggcg aggtcggcac gcggaatgcc
     5761 ctcggcggcg gcgtccgcat caaaaacgga gaggaggacc tgatcgtcgg ccagaaggtc
     5821 gctggagatg tccgattcct cgacacggat ctcgggcgcg gtgccccggg tggccagatc
     5881 ggcgaggcgg cgggagatct ggcgggcgcg gacggccggt gaataggcct tgaacggggc
     5941 ggcgatgcga aagacctctt cgccgcccag gaggacaggg gcgctgtcgg cggcgggcgg
     6001 aaggtcgggg ggggcggact ggactttttg tgggtgggac gacgcgggtg cggcgaaggc
     6061 gggcagcgcg gcgagggccg tcagcaggcc ggcccacagg atgaggaggc ggaggacagg
     6121 gcgggtgaag ggagggacca tgggcgcgct ccgggcggac aaggctccga ctataccccg
     6181 gcgcatcccg atcgggtacg ccgggcggct ggccgcgccg gggcctacag cagtgcaggg
     6241 agggctgcgc tgagggtctg cttgcgggcg gtggcgggac cgagcagtgc aaagccgcgc
     6301 agactgccgt cgggggcttc gaagcgggct tcgaggcctg cgtccggggt gtcttcgacg
     6361 atccgccaga cgcccgccgc gccgatgggc ggtggcgcga cgatggtggg gatcgccggg
     6421 gtcttcacca gcaccggcat gggcgggtag ctcacgtcgg tgggggtgcc cgtcaggctg
     6481 cgggcgaggg cccgggcctg ctgcatcagg ggcagcacga agggcaggtg aaggccgtcc
     6541 acttcggcgc agtcgccgag ggcgaagatg tgcgggtcgc tcgtggcgag gcggcggtcc
     6601 accgtgatgc cacgcccgac ggtgaggcct gcggcagtgg ccagcgcggt gcgcgggcgc
     6661 aggccgacgg cggacaggac gaggtctgcc ggctcctggc gaccgtcgcc cagttcgacc
     6721 cggtagccgg gtccgtcccg atgcacggcc tgggcggcct ggcccaggtg gaagcgtacg
     6781 ccggcggctt cgaggcgggc ctggaagtgg cggcctgcgg cgtccggcag caggcggccg
     6841 aggggccacg gggcgacgtc gaagacgctc gggctgatgc cgcgggcgag gaggtcgttg
     6901 gcgaattcgc agccgatcag gccggcgccg agaatcacga cgcgctgtac gccgtccagg
     6961 cgggcactga agcgggcgaa gtcgtccagg tcgttcaccg agagcacgtc gtcggcgccg
     7021 tcgccggcga gcggcaggcg gatcgggtcg gcgccgaggg cgaggacgag gtcgccgtag
     7081 gcgaggggct cgccgccatc gagctgcacg tgccgcgcgg ccgggtcgag ggacgccacg
     7141 cggcggcgcg gcaggatcgt cgcgcccagt tcctcggcca tccgctcggc ggacttcatc
     7201 accagggtgg tggcgctctt gccggcggcc agggcgttgg agagcatggg cttggagtag
     7261 aagggcgcat cgtcgcggct gacgagcacg aggggccggt cccggtcgag cttgcggact
     7321 tcgcgggcga gggtgtagcc ggcgaggccg gtgccgagga tgaggagggg ggcggtcatg
     7381 gtgggcgtcc ggtgcagtgc ggcgcccgcg cgggggcggg cgccagcggg tcagagctcg
     7441 atcatctcga agtcggactt ggcgacgccg cagtccgggc aggcccagtc gtccgggatg
     7501 tcggcccagg cggtgccggg cgcgatgcct tcttcgggaa gtcctgcggt ttcgtcgtag
     7561 atgaagccgc acacgatgca ttgccaggtt ttcatggtct tgattccttg tctcaggtag
     7621 ggggtgggtt caggcgtcga tctggtcaag gcgcgcctgg tagtgggcgg cgtggcgctc
     7681 ttccaccttg gcgagggcgg cgaagcgctt ggcggccttt tcgaggacgg ccttgaactg
     7741 ctcggcgtgc tcgcgggatt cggcgatctg ttcgtcgatt tcctgcaccg cggcgctatg
     7801 gccttcggcg acggcggtgt ggcggaaggc cgggtacatc tcggtgtact cgtaggtctc
     7861 gccttcgatg gcgaattcga gcgcgcgggc ggggctcatc ttcgccttcg ggtagaggag
     7921 gtccaggtgg ccgaaggcgt gcatgacctc ctggtcggcc gtcgcctcga aggcgcgggc
     7981 cgtctcctcg tcgcccatct cgcggcagag cttggcgaaa tagcggtact tgatgtgcgc
     8041 catcgactcg ccggcgaagg cggcttcgag gttctggacg gtggccgagg cggggtcgtg
     8101 gaaggggttt gccatgatgt ctctccttga aaagtgtcgc aggcttgcga tggagagatg
     8161 ctacggcggg ggtctcgatg tgtgaaattg attgttgtta gtattaagat agtcggtgcc
     8221 tatttaaacg ccgctgcggg cttcagtctc cgaacgggtg gtcgtaatcg acggtcagcg
     8281 gcgcgtggtc actgaagcgc tgctccttgt agatggcgca gcgccggacc tgcgcggcga
     8341 tgccgggggt ggcgatctgg tagtcgagcc gccagcccac gttcttggcc caggcctggc
     8401 cccggttgga ccaccacgtg tagcaggcat cggtggcgtc gggatggagg cggcggtagg
     8461 tgtccaccca gccctgttcg tcgaacaggc gcgacagcca ggcccgctct tcgggcagga
     8521 agccggagtt cttctggttg gagcgccagt tcttgaggtc gatggcctgg tgggcgatgt
     8581 tccagtcgcc gcagacgatc acctcccgac cgctgcagcg cagctgcgcc aggtggggca
     8641 ggaagtgcgc catgaacctg aacttggcgg cctgccgttc gtcggaactg gaaccagacg
     8701 ggagataaag tgaaataatc gacacattgc caaaatcggc ctggatatag cgaccttcgg
     8761 catcgaattc aggaatgccg aatccgtata tgacctggtc gggaattttt tttgcatata
     8821 tgccgacgcc gctgtagccc tttttctcgg caaaggaaaa gaatccccga aatccattgg
     8881 gggtgaccgt ttccggcggc aggtcggcgg actgtgcttt cagctcctgg aggcagacca
     8941 catcggcctg ctgcaaagcg agccagggga ggaagccttt cacgtttgcg gagcgaattc
     9001 cgttgaggtt ggctgtaacg acgcgtaaca tttgacttaa tttggaccgg tttatgggtt
     9061 ggattgccag tggattttag ccgtgatttc atccagcttg cctgtgacaa gggcgtcttg
     9121 cgtttcggcg agttcgtcac caaggccggc cgtctgtcgc cttatttctt caatgcgggg
     9181 ctgttcaacg acggtgaatc cttcggccgt ctgtgcgact tctacgcccg cacgctgatc
     9241 gcggcgcatc tgccctgcga catgctcttc ggtcctgcct acaagggcat tccgctggtc
     9301 gccggcacgg cgatgcggat ggccgagcgg ggcgtgaacc tgccattctg cttcaaccgc
     9361 aaggaagcca aggaccacgg tgagggcggt accctggtgg gggcgccgct ggccgggcgg
     9421 gtggcgattc tcgacgacgt gatttccgcg ggcacctccg tgcgggaatc cgtcgagatc
     9481 atccgcgccc atggtgccac gccggcagcg gtcgtgatcg ccctcgaccg catggagcgg
     9541 ggcaccggca gcctgtcggc cgtccaggag gttcagcgca cctacgacat tcccgtgctg
     9601 gcggtggcca atctggcaga cctcatcgcc tacctcaagg acagccctgc ccttgcggaa
     9661 aacctgggcg cggtcacgcg ttaccgggaa acctatggcg tggcgcggga ggcctgagcc
     9721 gtgatgatgc gccggctgct tttctgtctg ctcgccgccg tgtcggtgcc cgccggcgcc
     9781 cagtcgtccg ccaaggcgcc acgggtgttc tgctgtcccg acgccagcgg gcatcagatc
     9841 tgcggggatg tgctgccgga gcagtgctat ggcaaaagct accgtgaaat gagcccacag
     9901 ggcggcgtgc gtcgtctggt ggaggcgccc ctcacgcccg aacagctcgc ccgccgggag
     9961 gccgaggagc gcgcccgcaa ggccgagcag gcacgccagc gggccgaaac acggcgcaat
    10021 cagtccctgc tggaaaccta ttccagcgtg caggacatcg ataatcgccg tgacaaggcc
    10081 atcgaggcgg ttcagtccga gctgaagcag gccgaagcgc gttatgccca gttgcaggcc
    10141 cgccggcagg ggctgacgcg ggaggcggag ttctaccaga agcgatccat gccggtggcc
    10201 ctggccaatt ccctgaggga atccgacagc gagctggccg cccaatggtc ggtgatcgag
    10261 gtcaagcgcc gagacatcga gaacatccgc gcccgatacg agcaggatcg ggcccgctac
    10321 attgccctga ccgatccgcg cgcgcccgat attcgctgag cgctgcgaag caatattcct
    10381 tttcaattat ttcgacagga cgccggtaac cgaataggac acggcgctcc ggcgaattgc
    10441 atcgccggtc gggatcaatt ggcggcgccc ctgcgagggg cgtgagcagg cgccgggagg
    10501 cgcattctgc cggcggtgct tccgggcgct aaaacgcacc gcgggtggaa tggctgcttt
    10561 tcattaaatg aaaacaagcg tgtcgatagg ggctgacatc cttttgggat ggtccgcgga
    10621 cgctggtcgc ccgaaggctg cgtgataaac tcctccgctg atgtcatggt gggtgaaggc
    10681 ggtcaagcca ttgcccgcgt gcccggcccg accggtttca gaaacgctgc cgattgcccg
    10741 actgctcatg ttgcaataca agatcgttcc cgtgacacct ttcgagcaga attgctcgat
    10801 cctctggtgt gacgcaagtc gtcaggcggc cgttgtcgat ccgggaggca atgtggaccg
    10861 gatcctggct gccgtccagg cgctgggcgt caccgtcgag cgcatcctgc tgacccatgg
    10921 tcatatcgat catgcgggcg gtaccgccga tttggcgcgg caactggccg tgcccatcga
    10981 gggcccggat cgggaggacg ccttctggat cgagggattg ccccagcaga gccgcatgtt
    11041 cggctttccc ccggtgggcg ccttcacgcc cgatcgctgg ctggccgatg gcgacaccgt
    11101 ccaggtcggt gaggaaaccc ttgatgtcat ccactgcccc ggtcataccc cggggcatgt
    11161 ggtgtttttc agtccttcgg cacgtctggc ggtggtgggg gacgtgctgt tcgccggctc
    11221 catcgggcgt accgattttc cacgaggcaa tcacgccgca ctgattgcct cgatccggac
    11281 tcgtctgtgg ccgctgggca aggatgtggc cttcattccg ggccacgggc cgatgtccac
    11341 gttcggcgac gaacgggacg ccaatcccta tgtgggggat tacgcctgaa atgccggtcg
    11401 cagcccgcag gcaggggatt ccggtgccga tttgacgttg cacatttatt gaaaaggtga
    11461 gccagcgtgc gattcgctat tctcgaagac gatcctgccc aaatggaagt gctgactggc
    11521 tggttgcgcg aagccgggca cgacgtgcat ggatttgcca tggcgcgcga actgatgagg
    11581 gtggccagcc gggagagttt cgatctcttc ctggtggact gggtgctgcc ggatctgacc
    11641 ggcgagcagg tgctgcgctg gttgcgcacc gagcgcaaca acgatacgcc ggcgatcttc
    11701 atcacgtcgc gggatgccga agatgacatc gccagcgcct tcagcgccgg tgccgacgac
    11761 tatctggtca agccgccgcg gcgcatcgag ctgctgtcgc gcatcgaggc cgtgctgcgc
    11821 cggacccagc cgcgccaggt cagccagctg ctgcaggtcg cgccctacag cttcgacctg
    11881 atccgcaaga tggtcacggt aaatggcgag ccggtcgaga tgaccgacaa ggagttcgaa
    11941 ctggcggcct tcctgttccg caacatgggc cgtctcctgt cccgcggcca tctgctggaa
    12001 gcggtgtggg gccgtaaccc cgatctggcg acccggaccg tggataccca tatcagccgg
    12061 gtgcgcagca agctcaatct gcggccggaa aacgggttcc gcctgacccc cacctataac
    12121 ttcggctatc gcctcgagcg ggtcgatgcg gcgaacctcg cgccggacgg tgcgcccgcc
    12181 gaaggcgccg acctgtcttc ccaggtctga accggcggga cccgcccggg cgggtcccgc
    12241 tggcacaccg gacgatgtcc tatttcttca gggcatcgcg gatttcccgc agcagcttca
    12301 cgtcttcggc ctcgaccggt gcttccggcg cgggcacggg ttcttcgcgt ttgagtttgt
    12361 tcatcgcctt gatggcgacg aagatggcgg cggcgacgat cacgaagtcg atcacggtgt
    12421 tgatgaacac gccgtacttg aacaaggggg cgcccgcctt ctcggcggcg tccagggtct
    12481 cgtaggtctt gtcacccagg ttgatgtaaa ggtgcttgaa gtccacgcca ccgaggagtt
    12541 tgcccagcac cggcatcagc agatccttga cgagggagtc gacgatcttc tggaaggcgc
    12601 cgccgatgat cacgccgacc gccagatcga ccacattgcc cttgagggca aattccttga
    12661 actcctggat gatgctcacg ctattctcct cgtgtcgggg gaaaggggac gggtagacta
    12721 cggcccgaaa cggcgcctac ccggaaagcc gtgccgatca tagcgatacg ggccatggag
    12781 cggcgggaat acggcaccgt tcttcacctt tttaaggcca gtttgcgaag tttcacatcg
    12841 cccgacctcg gcgaaccgac tatattttgt tccttcgacc gatccgcgag acctgacctt
    12901 ggcccttcat cgacatcgca tcctgtccgc cgtactgggc gtcctggcct ggagcggcgg
    12961 cgcgttcgcc gcggacgagg acattgtcta cacggctcgc ccgggcgata ccctgatcgg
    13021 cttgcagaaa cagctgctcg ccgcaccgtt tggctggaag ggggtcatgc tgcacaaccg
    13081 catcgccgag ccgcggcgca ttcccaccgg cacgcaggtg cgcatccccg aagcctggct
    13141 gcgggtcgag gcgcgatccg cccaggtggt gggcgtgcat ggggaggcca ccctcaacgg
    13201 gcgtcccctg gccctggagg atcaggtgcc gcccggggcg gtgctgcgga cggggaacgc
    13261 gtccttcgtc acgctgcgca tgcccgacga atcgcgtctg accctccagc cggacagcga
    13321 ggcacgcctc gagaagatcc aggggtttgc cgggctcacc ggccagacgg cccagatcca
    13381 ccttgaacgg ggccgcatcg agaatacggt tgcggcccag cgtggcccgg cggcccgtta
    13441 ccggatccgc acgccgagtg cgagcgtatc cgtgcggggc accgaattcc gcgtgggttc
    13501 cgatgggacc gatgggcgcg cgcgcgccga agtgaccggc ggtgaagtgc gcgtcggcgc
    13561 cgagaccggg ccggccaccg cggtggccat gggcttcggg gtcaccgccg aacctggcgg
    13621 aggcgtccag ccgccgcaag ccctgctgcc ggcacccgcg ctggaaggtc tggcgaccgt
    13681 ccatgaacgt accgccgtgg agatcacgct gcggcctgtg gacaaggcgg tggcctaccg
    13741 tgcccagctg gctcgcgatg cggcattcac cgacgtggtg acggaggccg tatcgccggc
    13801 gccgccggtg cgcctcgaaa acgttccgga cgggcgcctg tgggtacgcg tccgcgccat
    13861 cgacgcggcc ggactcgaag gcttcgacac ccagcgtgcc ctggaagtgc gcgcccggcc
    13921 ggtgccgccc gacgctgccg ggcccttggc cggcgcggcc ttcgtcggcg ggccggtcca
    13981 tttcgagtgg acgccggtgg cggatgcacc gggttaccgc ttccaggtgc tgcggcgtgc
    14041 tgcgccggag gtgctgctga ttgatcggga ggttgttacg tcgggcatcg atctgacctt
    14101 gccgcggggc gagttccgct ggcgtgtggc caccctgaag cctgccggcg ggcagggtcc
    14161 gtggggcgac gcccgtgcct tggcggtgac gcgccccatc ggtgtgccca cggtccgccg
    14221 ctacaacgac cgcctgcgat ttgaatggag cggcgacgac ggtcagatct atgattttca
    14281 gctcgcccgc gacgccgggt tcggcgatct ggtggtcaac cagcgcctgg gcgaggcggc
    14341 catcaccgtg ccggtgccgg cgggcgggcg ctatttcgtc cgcgtgcgcg gcaccgacgc
    14401 ccagggcgcg gcgagtccct acacgccggt gcaggttctt tacaggctgc ccctgctgat
    14461 gtggaccctg tcgaacgcgg cgccgtgaag tcgggaaccg ggaccgtctt gcgccggccg
    14521 acactggagt ggctggccgt cacgacgctg tgcatcgtgc tggcggtgtt tgcggccctt
    14581 gagggctggc tgtggcgcat cgatcagacg atctacgaca gcttcctgtc cagcttccgt
    14641 cgggccacga gtgccaacgt ggcgatcgtg gctatcgatg acgcgagcct ggccgaactg
    14701 ggaagctggc cctggtcgcg gcgggtgcat gcggcgctcc tcgatcgtct gcacgatgcc
    14761 ggggcgcggg cggtggcgct ggacctgatc ctggacaccc cgtcgcggga cgatccggcg
    14821 gccgacacgg tgctggccca gtccatgcgg gccttcggcc gggtcgccct gcccctcacc
    14881 caggccacga ccggcgtcct gctgaccggc gagatcctgc ccctgcccga attcgtcgcg
    14941 gcggcggctg cactcggcca cgggcaggtg gagttcgatc cggacggcat cgcccgcagc
    15001 gtctatctgt gggaaggctt tggcagtgcc cgccatcccc agttggcgct ggcgctgctc
    15061 cgcatcgtcg atccggaggt ggccgcccgc tatgcgcagc cgggccccgg cgatgacgac
    15121 gatcgtctgc aatgggtgcg cgccacgtgg ctgcgggtac ccttcgccgg gccgccgggc
    15181 acctatccct attattccta cgccgcggtg ctgcggggcg aagtgcccgc cgacgccctg
    15241 cgcgacaagg tgctgctggt gggcgccacg gcctcgggga tggccgaggc cgtcccgacg
    15301 cccatgtccg gcctgtcgcg gcccatgccc ggcgtcgagg tggacgccaa cgtttacgac
    15361 gccttgcgcc ggggagatgc cgtccggccg ctgtcggcgg ggacctcggc cggtgtggcg
    15421 gccctggcgg tgctggtcgc gatgcttgcg ctgttctacg gggatccgcg caccggactg
    15481 ctggtgtccg tcctggccgg gctggccgcg ctggccctgg cggccgtggc gctgcaggcg
    15541 ggcagcatgt ggttcgggcc ggctcccgcg gtgctgggct gcatgctggc gtatcccctg
    15601 tggagctggc gccgcctgga atgggcccag cgctatctgg atgcggagct ggccgcgctg
    15661 gagcgagaag gggacgcact gccgtcggag gtggatcggg gggcggcgga tcgcctggag
    15721 cggcgcatcc gcatcgtccg ccacgtggcc cggcagcagc gggatgtccg ccgcttcatt
    15781 gccgacaccc tcgagcacct gcccatcggt gtggtggtca tcgatccggg caaccgggtg
    15841 gtgctgcaca atgagcgtgc ccgccatctg ctccaggcct tccgtggcga agacctgctg
    15901 gcggccttgc gcagcctgcc gtggcccgtg tatctgccga tgcgcgacgg cctgccggac
    15961 ctggccgggt ttgccggcat ccgccagttc gagctgcccg tggggcggcg ccatctgctc
    16021 ctgtcggtgg ccgagctggt ggccggtacg ggcagcctgt ccggccgcgt gattggtctg
    16081 gcggatgtga ccgtcatgca cgaggcccag cgtacgcggg aggacaccat gcatttcctg
    16141 tcccacgacc tgcgcgcgcc gctggcctcc atcctgacgc tcatcgacgg ctacaagggt
    16201 gatccggccg cccaggcgga acagattccg cgcatcgccc gctacgcccg ttcggcgctc
    16261 gatctggctg acggcctgtt ccgtctggtc cgtgccgagg ggtctgaccc gaaggatttc
    16321 gtggagctgg acctggccgg tctggtggat tccgccgccg acgaggtgtg gccccaggcc
    16381 cacgcccgcg gcatccgtgt cgccgtggac acgagggcgg cggagaccga ggagcccatc
    16441 gtgcggggcg acttctcgct gctgcgccgg gccgtgatca acctgctcac caatgccatc
    16501 aagtacggca gtccggacag cacggtgagc gcggagctcg ccgccgaggg cgccgactgg
    16561 gtgatccgcg tgcgcggcca gggggctggc attccgccgg atgcgctgcc gcgcctgttc
    16621 aagcgcttca gccgcattgt ggaggaaggg cgcgaccggc ccagcggcgt cgggctgggg
    16681 ctcctcatcg tcaagaccgt ggccgagcgt catgggggca acgtgtccgt cgagagcacg
    16741 ccgggggtcg gcagcatgtt cgagatccgg ctgcccctgc gcatgcgtcc ggacgcgcct
    16801 tcgcgcccgg attgagaccg ggcgcggcgc tgggtgaagc acggacgtcc cggacatggg
    16861 tttcaaaccg ctgaagctcc tggctttcct gctgggcggg ctgagcgccg tgatgggggc
    16921 tggggccctc tacctgtatg cgaccttcga tggcgcccgc ctcgccgctg agctgaccca
    16981 gtccctgcgc cagcagaccc agcgtaccgt gcgcctggac gggccggtgg ccttgtcgct
    17041 gtttccccgg ctgagtctgc gcctgccggc caccaccgtg tcggatccgc gcagcgacgg
    17101 ggcattcctc gcgtttgagc gggccgagct gcgtgtcggt ctgtggccgc tgttgtcgcg
    17161 gcgggtggcg atcgaagcgg tcgaactgca gggcccgcag ctcacgctcc ggcgccgacg
    17221 cgatggggcc gtgaacaccg acgatctggc cgcgctgctg cagggccagg agggcgacac
    17281 ggccttgccg ctgacggccg atgtgcaccg cctcgtcgtg catgacggcc gggtggactg
    17341 gctggatgag gcgggcggtc gtcgcctggg cgtcaccggg ctgacgctcg agacggacga
    17401 tctggcgtcg cagtcggtcg ggcgattgag cctgacaggc cacctgcgcg gcgattcacc
    17461 gaacgtggag gtggacctcc gcgtggccag cgactatcgc ctggacgcgg tgggcgggcg
    17521 tcatgccctg cgtcatctga gcctgtcggc ggtcggcgtg ctggcgggcc gtggagacct
    17581 cgaggcgcgg gcgagcctcg acgagctggc ctgggtgacg ggaggcgagc ttgtggtcaa
    17641 gggtgcggcg ggcgatctgc gtgcacgggc tgcggagggc gccccggtcc tgcacctggc
    17701 gacgcccgac ctgcgcctca cggcgaacgg cccggtcgcg gcgacggtgg acgcggacgc
    17761 acgctggggg agcgaggcgg agggcggacg tctgacggcc cgtctctccg atctgcaggc
    17821 gcgagcggct ggcatcagcg tgacggcgga tctccgcaca ggggatgacc gcctgaacct
    17881 ccaggggcgc ggcgctggcg actggcgggc cgcggagtcc ctcgcacagc tgcgtggcgt
    17941 ggtgctcgaa ggaacctgga cgcggcgcgg tggcgcgtcc agtccggtgc gggccggtgc
    18001 agaccttcag gtggatacgg cccgcggcag cgccggcggg acggtcgatc tgcgcatcga
    18061 cggcagccgg atcaacggca cctgggcctt gagccggttc gcgccgtggg ccggtcgctt
    18121 tgagctggag gcggaccgcc tggacgccga ccgcctgttt gcggcaacgg gcgaccgctg
    18181 ggatctgtcc ggcctgcacg ggttcgatgt ggacggtcag ctgcgcatcg gcagcctccg
    18241 cgccgcgggg ctgcagtttg agcgcgttcg tctgcccctc agcctgcacg gcggccggct
    18301 ggtgtcgcgg gggcatgctg ccgggctgta cggcggcgcc gtggcgggca atttcaccct
    18361 ggtggccgat ggcaacaagg cgttctacac cggccagatc aatggcgtca gcgtggagcc
    18421 gctgctgcgc gatgccaccg gacgggaggc cctgagcggc acgctgaacg cgttcgtgga
    18481 tgtggcgacg gcggccgggc ggcgatcagc cctcgcgggc gccctcgccg gcaaggggcg
    18541 ctttgtgctg ggtcacggcg ccgtgcgggg gatcgatccg ttcgcggcgc tgcgcgaatg
    18601 gcaggcagct attgccgctc gccgtggggc ccggcggacg tttcgggacg ctgagcaggc
    18661 gggcctgacg gaatggtcgg gcagtttcgg catcgagcgg ggcgtggtga gcagtgccga
    18721 tctgcaggcg cgcagcgccc agtggcgggc gagcggacac ggcgtgctgg atctggcggc
    18781 cggccgcatc gacgttcagc agcgcttgac cgtgctggcg ctgccgccgg gggcggaggg
    18841 cattgcgctg gcgtccttgc gcggggcgac gctgccggtg cgggtgacgg gtccgctggg
    18901 gcgcgccgaa tggcttctgg agccgtcgtc cggggtgcag tccgccccgc ctggcgggcc
    18961 gggagccggg cgtggtccgg cggacggagg gcggcgatga atgggcgcgg cgcgcgggtg
    19021 ttcaggcgac cggatcgcgg tccggctccg cggcctccca gcggctgcgc ttgatgcggt
    19081 acatctggcg gtccgcgtgg gcgagcaggg tgagcggatc gcggccgtgg tccggataga
    19141 tggcgacgcc gatggaacag ccgatctgga cctgaccggt gccgaccggg atgggcaggg
    19201 cgattgaacg ctcgatgttg gcggtgagcc gcttgagcat ggcttcgtcg cccgcgtcgt
    19261 ggagcaggat tgcgaattcg tcgccgccgt agcgggccac cacatcctcg ccgcggacga
    19321 cccggcgcac ccgtccggcg acggacttga gtacccggtc acccgccgcg tggccgtggg
    19381 tgtcgttgac cgccttgaac tggtccagat cgatgaacag gatggcgatc ttctggccga
    19441 cttcccgcgc gccggagacg gccgcgcgga cgagcacgtc gaagcgctcg cggttgagca
    19501 ggcgggtcag gctgtcgtga ttggcgcgaa attcgttgcg cacgacctgc tcgtggaatt
    19561 cggtgacatc caccatggtg ccgatgaagc cgccgatgcc gctgccttcc cgcacgacac
    19621 cggcctcggc gcggatccac aggtcggagc cgttgggatg gcggtagcgg aactgctcgg
    19681 agaaccggtt gccggtctgg acggcccgcc gccagcgggc ggtcatgcgg tccacgtcat
    19741 cgggtggcag caggtgggtc cagggttcgt cgggcagatc ggccgggctg cggccgagca
    19801 tctgggcgca gcgttcgttg aggaagatga ggcggccctg ggcgtcggtc tcgaacagtc
    19861 ccgcgggcgc ctggcgcgcc agttcgcgaa agcggagttc gctgcgctgc agctcggcct
    19921 gggcggccga cagccgcgcc agatcctcct gcaggcgccg cgcctggctg cgcagcaggg
    19981 cctcccggcg gaccgtctcg gtgacgtcgc gaacctgcag caggcagctc cgcccgtcgc
    20041 cgtcgtccac cgggctggcg tcgatcatct ggcgcagacg gggcgcgccg ggatctgccg
    20101 gattgtgcag gggcaggggc gcagggtgga agacctgaga caggtgcgag gagcggccga
    20161 aatcaatggc ttcgcggatc gccaccgcga tgcgcgggtc catcagggtg ccgaacgcgg
    20221 tactcaggcg cgaacccagg atgtcacggg caggcagtcc gctgaacgtg accatccagc
    20281 ggttccagtg ctgcacccgc ccatcccggt ctaccaggat gatgccgacg tccagggcat
    20341 cgagaagggc gggggtgctg ctcatgacgc gctcagaccc gttcgggcag attgaagagc
    20401 cgcgccactt ggcgcatgaa ggactcgatg gagggcacgt ccatgatgaa caggacgtag
    20461 ccggaaatgt cgtgggccga catgcgcatg ttgatctgcg ccaccaggat cacgtcgctg
    20521 gcctggcgca cgccgagcag ggtgtcggcg gtgcaactgc gcaccaccgg cagcgaaccg
    20581 tgcatttcgg tgccgaacag gttggcgaga ctgctcatgc agctgttgat gatgatgttg
    20641 ccgatctcgg cgagggcgtc ctgctccagt tcggtgatct gctcgatggg cgtctcgtca
    20701 ccgatcatgc ggcgcacgat ctcgaggctg ccctgttcgg gaaacagcag cagggcgtcg
    20761 ctgtcgaagt gcatgtccgc gtggaagcgc tggtcgatgc tgcagatccg tgaggtgcca
    20821 ttgccgcagc cggccgcctg gaggcgggcg atgagggtgt cccgagtgat gatctccacc
    20881 accggagccg acagggtgat ctcttcgcgt acgatcgatg caaaggtggc ggccgcttca
    20941 cccagtgcga ggttgaaggc ttccgacaat gcgtcgcact ccagctcgct caggggtctc
    21001 atgggtgcga ctccagcagg tcgaggatgc ggccaaccac ggtgtcgttg atcggcttgg
    21061 caaaaaaggc tgcattgagt gccgccgcgc gctggcgtac gctgtcctgg acgttggcgg
    21121 tgagcagggc gatgcgcatg tcggggtggt cgttgcggat ccgttcggcc agttcgagac
    21181 cattcatgcc cggcatgttc acgtccagca cggccaggtg gggtaccagg gtggcgatgc
    21241 acgagagggc ctcgtcgccg ttgccggctt cgacgaggat ggcatcgggg cggcgctcgc
    21301 ggacgatggc gccgcacagc attcgggaaa cccgactgtc atcaacgagc aggatcaccg
    21361 gcgcagcagt catggcagac ctccagatca tgacgctccg taacggccga acctgtcatg
    21421 gcttgaggga gcttgagcat ttttttcatt ttcggcggag tgcctggatg aaatgccaga
    21481 aacgatgatc gttgatgtat ctggaatggg catcgaggtg ccgcacgggt gggctccggg
    21541 cgcgcgtctg cgtcgggacg ccggcagcac cggagcgggc acggcggtgg ccgggaaagg
    21601 ggtgaggccg tgaatttcct cgcccatgcg tggctcgcgg ggccccatcc ggccgatcgc
    21661 ctggggggcc tgatggggga cttcatcaag gggccgctgc ccggcgcgct ggcgccggcg
    21721 gtggcgtcgg gcgtacggct gcaccggcgc atcgacgtgt ttgcagacag ccacccggca
    21781 ttcctgcgca gccgggcgcg ggtcagcacg gcgcgccggc gggtcgccgg agtgatggtg
    21841 gacatgtttt acgatcactt cctggcgcgc gactgggcgg aattccatcc tgaaccgctg
    21901 gccgacttca cggcggccca gtaccggctg ctggaggagg cgcccgggct gcccgagcgt
    21961 ctgcagaaga tcctcccgtc catgcgggac gatgactggc tggcgtcgta ccgcgacgtg
    22021 gagacggtgg cgtacgcgct ggagcgcatg gcaggccgcc tgcggcccgg caacccgctt
    22081 gcgggcggcg gtgccgagtt gctgatgcac tacgcggaat tcgcggcgga ttgtcgcgaa
    22141 tttctgccgg acgcgcgggc gttcgcccag gccttctggc agcgcgacgg cttcagcgca
    22201 gcggaatgac gaggggcgga atgccgatca cgacggaggg ctgcggcgtg taatagaccg
    22261 gccgcggcgg cgcggcgtag tacaccggcg gcggcgcgta ataggcctgg ggcgggacga
    22321 ccatgacggg ttcgcggacc gggtaatagt gatggtgccg cggtccccag ccccacgcgc
    22381 cgtggtgatg cccttctccg ccgccatggg cataggcggg cacggtggca gcacatccca
    22441 gcgtcaggag cagggcggca gcgagcgacg ggtggcgttt catggcgatt tctccttgtc
    22501 ctccgccgat agggaggttt caagggcagc ataggcgcag gcggcgatgg gcgttgtggg
    22561 cctttgtaag caaaattggg gaaaaccacg ccggtgcgtg gcgggtgtcc ggcgccgtgg
    22621 cagggcggag cgggccgctt ccgtgcgtgg cggcttacaa atgctgaggc ttttgcaggg
    22681 ggtggatcag ccggtgggcg tctcgcccag gtggcggcgc cagcgttcga tggcggcacc
    22741 gagggcttcg tggctgacag gtttggtcag gtagtcgtcc atgccggcat cgagggcgag
    22801 gcggatgtcg tccggcaggg tgtcggcgct gcagcccacg atgtaggcgc gacagccccg
    22861 ttcgcgcagg gcctgggtgg cctcgatgcc gctcatgacg ggcatgcgcg catccatgaa
    22921 gatcaggtcg taacggtctg ccgtgcccag gtccaccgct tcccgcccat cccgcgcgaa
    22981 cgccggttgg gtggccccca ggcggctgag gaggcgggcg agcaccacct ggttcaccgg
    23041 gttgtcttcg gcgatcagga tgcgggtgtc gcgacgcagc agggcgcggg gcgtttcgac
    23101 ggtcgggcgt tcttccgcga cgcccacgtc gagggccacg ctgaagtcga aacgggatcc
    23161 ggccccgacc tgactgtcca cgcggatttc gccttccatc agggtgcaga gcatgcggca
    23221 gatggcgagc cccagcccgg tgccgccata gcgccgggtg gtggacatgt cggcctgttc
    23281 gaagggcgtg aagatgtgct cgcacttgtc ggccgggatg ccgatgccgg tgtcggtgac
    23341 cgcaaagcgc acgatgacgc gaccgccgcg gagcgcttcg gccggttcca cgtcgatgcg
    23401 gatggccccc tgcggggtga acttgaaggc gttgctcacc agattcacga ggatctggcg
    23461 caggcgcgtg ggatcgccca tcaccacgcg gggcagggtg gtggccaggg tggcgccgaa
    23521 ctggaggccc ttttcgaggg ccttggggcg gaacatgcgc tccacgtcgg tgacgagctg
    23581 gtgcaggtcg aagctgatgc gttcgagggt gagcttgcgg gcttcgatct tggaatagtc
    23641 gaggatgtcg ttgatgatgc ccatcagcgc atcgctgctg gtcaggatgg tgtcgatgta
    23701 ttcccgctct tccggacggt gctcggacat gccgaggagc tgggccatgc cgatgatgcc
    23761 gttcatcggc gtgcggattt cgtgggacat gatcgccagg aagtcgctct tggcccggtt
    23821 ggcagcgtcg gcggcgtcgc gggcggcgat gagggcctgc tggctgcggt ggcgctcggt
    23881 gacgtcggtg aagcagaaca cgcggccgat cacctgctcg gcctggcgtg ccgggcgcga
    23941 ggtgatctcg caacgacggc cgtcacggaa gacgaaggtg tcgaaggtgt cggtcagatc
    24001 gaacagatgg tccgtgagca ggctgcggaa gggcgccggg ctttccagcg cagcggccat
    24061 gtgcgcgatc agggcgtcgt cgtcgcggtg ggcgagcagg gtttcgggca gttgccactg
    24121 gcgcgcaagg cggcggttca tgttgaccac ccgtccgtcg gcgtcgatga ccaggatccc
    24181 gtccgccgtg gcctcgaggg tggcgcgcag ctgggcggtc atcagggcca gttcggtttc
    24241 ggcctttttc tgggcgccga tgtcggtggc ccgcaccagc atgccggcgg gcacacggcg
    24301 cacgcatttg cgggcgctca gcagggtgcc gtcggcgcgg cggtagaggc cttccatcac
    24361 ctgttcgtcg gcgccaccgc cgctacgcac ctcttcccag aagaacaggt ccgcaagggc
    24421 gcattcgatc tcggtgaccg gctggccgac gagggcctcg gcgcgcaggc cgaggaggcg
    24481 ctcggcggcc ggattgacgg cgaggatggc cagggtggcc gggtctacga gcatcaggag
    24541 ctcttcggcg cccgcgagga ggatggcatc gtgggcggca ctcactgtcc gcgcctccgg
    24601 ttggcgtcga agtagtaggt cacgcgcttg cgcgggctca ggtactcata gacctcgggt
    24661 ggcagcacga tggggcgcag ggccttggcg atgttgatgt cgggttcctg ctccagatcg
    24721 agcatgatcg cctcgtcctt ggggatatcc ggatcataga ccgtgaccca cgggcgcagc
    24781 ggcttttcgg gattgaccga ggccaccagg gcgagggcgt cgttggacag cttgaccacc
    24841 gtgcccggcg gatacacgcc caggcagcgg atcatcacct gcagcgcctg ggcgtcgaag
    24901 cggccgcggc gctgggcgaa catcagggac agtgcctcgt ggggcgtgag ggccttggcg
    24961 aggtcgcccg gattgcacag gttgtcgtaa tagttcacca gggccaccag gcgggcgcag
    25021 gggtcgatct ggtcgccctt gagggccttg ggatagccgc tcccgtcggc catttcgtgg
    25081 tgctgggcga tgatgcgcag gaccggctcg ggaaggccga tctggcgggc gaggcgcacg
    25141 ccgtagtcgc agtgcatctc gcgcaggtgg cgttccggcg gggtcagttc gtggcgggcg
    25201 cgggcgatgc ggtcggggat gtccaccagg ccgatgtcgt ggaggagggc gccgacgccg
    25261 agggtctggc cggcggcgcg gggcaggccc agctcgccgg ccagcatcat ggcgaggatc
    25321 gagcagttga ggccgtggta gtaggcctcc tcgccgccgg cgtgttcacc gatcacgtgc
    25381 agggcgactt ccggctggtc caggaaggcc accaccatct ggttgaccag ctcgtccacc
    25441 tcttcaagac actccttcgg ccgcgcgaag aggttcttgc cgatgtcgcg catcacgttg
    25501 gcggccttca cgaaggcctg ttccacctcg atgacctggc gtttgcgctg ttgcagacgg
    25561 aggatgcgtt cgcgtttggc ggccagcgcc ggatcgtcgg cggcaggcgc ttcagggggc
    25621 ggcgcgggcg cgtcccgagg ctcggaggcg ccgacggccg ggggcaggtc ggagcgggcc
    25681 ggatcgcagc ggaagcggct gacgcccagt gtgcgcagcg tggcgatctg ctcgtccgac
    25741 ttgatcttga agctgttgaa gctgaagggg tggctgaacc acggcaggtc cacgtgaatg
    25801 aacagcccga cgcacagctg ctcgggcgtg acgaagacat cctgcggtga ggcggtatgc
    25861 ggcatcgggg gcacgacggg acggaacgag tctctttcgg cccggatgct ccgaagttga
    25921 gggttttgac agttttgcgt tccgatcgag ccccgcggtg gcggaaaagt gcgccgacgc
    25981 cccggcggac cggggcgcgc gggcgctcag gggcttattt gcggccgaac ggttcgggcc
    26041 gcggcgtcgc gtcggacgag gggggcagga tcggcagctt gaagttcggc tgggcaccgg
    26101 tgagggtctt caggaaggcg acgatccggg cgttctccgc gtccgtgaag tgcttgccga
    26161 gctggatgcg gcccatggta tcgacggcat ccttcagggt ggtggcggcg ccgtcatgga
    26221 agtacgggta ggtcagttcc acgttgcgca gggtgggcac cttgaagttg aagcggtcgg
    26281 catccttgcc ggtgacggcg ctgcgccctt cggccgggct gctggcggtg tagggggcga
    26341 cgacacccat cttctggaag gaattaccgc ccaccgccgg gccgttgtgg caggcgacac
    26401 agccgctgtc cttgaagagc gtgtagccgg cgacctcgtc ggcgttgagg gccttcttgt
    26461 tgcccttgag ccactggtca aaacgggaat tgggcgtgaa gagggtttcc tcgaaggcgg
    26521 cgatggcgcg ggtgatcttg tcgatatcca ctttgtcgct gccgaagacc tgcttgaact
    26581 cggcgctgta gccgggaatg gactggatca cctcgacggc cagcacatgg gtggaggcca
    26641 tctcgcccgg attggcgatg gggccgccgg cctgggcctt gagatccggc gcacggccgt
    26701 cccagaattg ggccaggttg aggctggagt tgagcacggt gggggcgttg atcggacctt
    26761 cctgccagtt gtggccgatg gaggtcttca ggttgtcggt gccgcccatg gcgaggttgt
    26821 ggcaggaatt gcacgagatg aagccggagc gcgacaggcg cggatcgaag aacagcttct
    26881 tgcccagttc gaccagggca ggggcgctca ccttggtcgg ggcaaggggc gtaatcggtt
    26941 cctgtggtct ggcgggggat gcggcaattg cggcggtggc gccgcagagg gacagggcaa
    27001 gggcgaggcg gcggacggac atgagggggt ctcccttctg gttagcaaca tggaatagtc
    27061 gttcagcata tgcgcggtgg ccgggggcgt gttgatgtgg gtcaaggtca cgggccggga
    27121 atcgccacag aaggtcacag ggcgggcctg gcatggcgtg gaggcatgct cgcgcgcaca
    27181 ccccagcccg gagcaaggcg ttccctgttc ccggggaatt cgatgggggg cacagctgga
    27241 cgcgtgcgtc gatggcccgg cgggttcggg ccgtcaggct ggcgttcggc ggggggcgag
    27301 acggtgcttg ctgtggatgg gctccgggcc gaggcgcagt gctgcggccc ggggcgaggg
    27361 gccggatggc ggattggccg tcaaccgctc acccgcggcc gagcaggctg tgacggcggg
    27421 ttcctgtgag ggcgtcggcg ctcaggggcg tcccgaccca tcctcgggca tgcgctcgtg
    27481 accgtcttcg gccggcgggg cgaccttgag gacttcctcg agcgtggtga gaccggccgc
    27541 caccttgagg gcgccggaga tgcgcagggg cttcatgcct tcgcggatgg cctgcaggcg
    27601 cacctgggcg agatcgccgc tgcccaccac gatgcggcgc agttccggcg acaggagcag
    27661 gatttcgtac aggccgatgc gccccagata gccggtcatg cggcattcca tgcagccgac
    27721 ggggtggtgc aggcggctcg gccgggcagc cttccacggc gtgacgaggt gatcccactg
    27781 ggcctcctcg tccggcgtga ggggcgcggc ggtcttgcag tgggggcaca ggatgcgcac
    27841 caggcgctgg gccatgatgc ccagcagggt ggcggacagc aggtaggcgg gcacgcccag
    27901 gtcgagcatg cgggtgacgg cggagggcgc gtcgttggtg tgcagggtgg acagcaccag
    27961 gtggccggtg agggcggcct ggatggccat ctcggcggtt tccaggtcgc ggatctcgcc
    28021 caccatgatg atgtccggat cctgccgcat gagggcgcgc acgccctccg caaagccgag
    28081 gtcgatgctg tgctggacct gcatctggtt gaaggacggc tccaccatct cgatgggatc
    28141 ctcgatggtg cacacgttca cctcgggcgt ggcgagctgc ttcagggtgg agtagagcgt
    28201 ggtggtcttg ccggatccgg tggggccggt gaccagcacg atgccgtggg gacgccgggt
    28261 gatggtgtgc cagcggccct cgtcggtgtc ggaaaagccg agctcggcga gggtgcgcac
    28321 cagcacctgg gggtcgaaga tgcgcattac cagcttttcg ccgaaggcgg tgggcagcgt
    28381 ggacaggcgc agctccacct ccttgccgct ggccacgcgg gtcttgatgc ggccgtcctg
    28441 aggccggcgc ttctcgacca cgtccatgcg gccgaggatc ttgatgcggc tggtcatggc
    28501 gttcatgacc ggcatgggca tctggtaaac ctgatggagc acgccgtcga tgcggaagcg
    28561 caccagcccc atgtcgcggc gcggttccat gtggatgtcg ctggcgcgct gctcgaaggc
    28621 gtactgccac agccagtcga cgatattgac gatgtgctgg tcgttggcgt cgtactggcg
    28681 gttggtctgg cccagctcca cgagctgctc gaaggacgac acgccgccgc cgtggttgcc
    28741 ctgcgcggcc tgcttcaccg agcgggcgag gttgtggaac tccaccaggt agcgggcgat
    28801 gtccagcggg ttggccagca cccggtgaat gcgtttcttg aggatgccgg ccagctcgcc
    28861 ttcccattcc cgcaggaagg gctgggccgt ggcgatcacc acttcctggg gcgtcacctt
    28921 gaccggcagg atcgcaaagc gcgcggcgta ggcggcggac atcactgcgg tgaccgcggt
    28981 gaagtcgatc ttgagggggt cgatgcggaa atagtccagg ctgacccgtc ccgcgagcca
    29041 ttcggtgagg gtctccaggt ccagcgggcg ggacggggcg ccacgagcgg tccactgctg
    29101 ctcggcgagc aggaccaggg gatggatctc accgcgcagg cgggccgggc cgttgaggaa
    29161 atcgtcggcg tcgggccgat ccaccagccg gtcggcgacc agcaggtcgg tgacttcggc
    29221 caggctcagg cggtgttcgc gcagggcgtc gggggggagg gcggcgggag cggacatgag
    29281 ggctgcctcg gcgggcggaa aggcggacgg gcggggcatc agcacgcccg cgcgccctct
    29341 acgatagcgt gccagcagag gccgtggcgg gacatttgca tgtctttacg cggtggacgt
    29401 ctcagcgcgt caggcgcccg ccccgtttgc gcggcccgac acccgccagg gcagccagcc
    29461 cggcgctggc atgggcgtgg cagatggcgc aggcgagggc gtcggccgca tcgggcgaag
    29521 ggctcgcctc cagggtcagc aggcgttcca ccatgtggct gacctgctgc ttggcggcct
    29581 tgccgtgacc gacgaccgcc tgcttgacct gcagcgcggt gtattccgcc accggcaatt
    29641 cgcaggacac ggcgccgcag atcgccgcgc cgcgggcctg gccaagcatc agggtggact
    29701 ggggattcac attcacgaag acgatctcca ccgccgcctg gtccggccgg taggtggcga
    29761 tgacttcccg caccccgtcg aggatcacct tgaggcgttg gggcaggctg ccctcgccgc
    29821 tgcggatgca gccactggcc acgtagcgca gctcgctgcc cagcgtgtca atgacgccga
    29881 aaccggtgat gcgcaggccc gggtcgatgc ccagaatgcg cgtggcgacg cgctcggtgg
    29941 tgaggctcat cgacgcgcca gccacacgcc gaaggcggtc agccccatgc cggcgagggc
    30001 caggccggtg agcgtctcgc cgaaggcagc ccaggcgatc agcgccgtgg tggggggcgt
    30061 gaggtagaac aggctcgcca cattcaccgc gctgccgctg cggatcagca ggttgagcag
    30121 gctgatggcg cccaccgaca gcaccaggac cagccacatg agcgcaaaga cgaaggaccc
    30181 cgtccacgcc accgtcaggg attccgtgtg ccaggccacc agggccgaca gggcgaggct
    30241 gggcacgaac tggatcaccg ccccggtgcg caggtcgaag gccgggcaga agcgcttctg
    30301 gtacagggtg ccgacggtga tgccggccag cgccgccagg gccggcacca gcatcgcggt
    30361 gagtccgtgg gcgtcggccg ccgccgccac cttgtgggcc accaccaggc cgaccccggc
    30421 gaagcccagc accagcccgg cccactggcg cggcagtacc cgttcgccca gcaggacgcc
    30481 ggcgccgagg gcggtgagca gcggttgcag gcccaccacc agggccgtga cgcccgcagg
    30541 caggccgtga cggatggccg tgaacacgcc gcccaggtag acgccatgga ccagtacgcc
    30601 ggtgaggccg atgtgcagcc actggcgcgg atcctgcggc cagggcgcgc gggcaagcag
    30661 cgccgcggcg cccatcaggg cgatcaccag cgtgtagcgg atgcacagga agaccagggg
    30721 ctccgcatag ggcagcccga acttggcgcc gatgaagccc gtgctccaca ggagcacgaa
    30781 gagcaggggg tacaggcggg tcaacgggcg aattcccggc ggacagcgac gccgccgggg
    30841 cgatcgggac gctgcggggg tgtcactggg ggccgatctt ggcgcggtag atgcgtgtgg
    30901 ccgcgctcac caccacctgc acgtcctccg gcggaatgcc cggcaggttt tcgcggacca
    30961 cccggtaggc caggtgttcg agggtctggc cgttccaggc attggcggga tcgaaaaagg
    31021 gctcgatgag ggcgtaggcc ttgtccacca ggtcgcgcag gtgcaggttc ttgcggcgtt
    31081 cggtcgggat gctcatggga gggggctccg gaagggggcg acgggccgtc ctggcgcagg
    31141 ggcgcaggac accggagccg atgataccct aggccccgag cttccaaatc cggtaaagca
    31201 ggagcattcc atgcgttttt ggctgatgaa gtcggaaccc gacgacgtgt ccatcgacga
    31261 cctggccacg cgcccgggcc agatcgtgcc ctggtacggc gtgcgcaact accaggcgcg
    31321 caacttcatg cgcgacgcca tgacggtggg cgacggcgtg ctgttctacc actccggctg
    31381 cgccgagccg ggcatcgccg gcatcgcccg ggtggccagc gcggcctatc ccgacgccac
    31441 ccagttcgat ccggcgagcc gatacttcga ccccaaggcc acgccggagc agccccgctg
    31501 ggtgcatgtg gacgtggcct tcgtgcgcaa gacccgcctc ctgggtttgt ccgaattgcg
    31561 cacacacccg gagcttgcca ccctacgcgt ccttgcccgc ggcaaccggc tgtcgatcac
    31621 gccggtggaa cccgccgagt ggcgcttcat cgtcggggat ctcctcggcg caacggatct
    31681 gaaggacggt tgatggaact gcactggctg gccgcctacc tgctgctggg ggcgtttgtg
    31741 ggattcttcg cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgcact
    31861 ggctggccgc ctacctgctg ctgggggcgt ttgtgggatt cttcgcggga ctgctcgggg
    31921 tgggcggcgg cgccatcatg gtgcccatgc tcaccaccct ctttgccgcc cagggctttc
    31981 cgcgggagca catggtgcac ctggccctgg gcacctccat ggcggccatc gtcgtcacct
    32041 cgatctccag cctgcgcgcc caccacgccc acggcgcggt gcgctgggag atcgtcaagg
    32101 gcatcgcccc cggcgtcctg ttgggcacct tcggcggctc cttcgtcgcc tcgcgggcgc
    32161 ccactgcgcc gctggcgctg ttcttcgcca gcttcacggc ctacgtggcg gtgcagatga
    32221 tcctcaacaa gaaacccaag ccgtcccgcg agctgccggg cccggcgggg ctggccgcag
    32281 tgggcggcgg catcggcttc atctcgtccc tggtggccat cggcggcggc tccctgtcgg
    32341 tgcccttcat gacctggtgc aacgtgaaga tgcagcacgc catcggcacc tcggccgcca
    32401 tcggcctgcc catcgccgtg gccggtgcgc tgggctacct gatcaacggc tggggcaccg
    32461 agggtctgcc ggcgtggacc gcgggcttcg tctacctgcc cgccctggtg ctggtgagcg
    32521 ccgtgtccag cctcaccgcc cccatcggcg cccgcctcac ccaccgcctg ccggtggcga
    32581 ccctgaagaa ggtctttgcc ggcgtgctga tcctgctgtc ggccaagatg ctgcacacgg
    32641 tgtttggcgg ctgagggcgt tcggcggcgc ggggggccgc tcaaggatga cacggggctg
    32701 ccgtggacgt ttctgatgac tgccacccgg tgcccgccat gtccgacgcg accgtccgcc
    32761 tgcaactgca tttcgagctc gagctcgcca cgccgcccga actggcgggg ctcgaccatg
    32821 cggccctgtg ccggaccctg gctgatgcgc tggggccgac ggtgctgcgc ggcttgccgg
    32881 cgatcgccgg caagcagctc acgccgctgg gggcgcgcgt ggtgcgccat caccatcatc
    32941 tggatgcggc ggcccaggcg gcgcgtcgcg tcgagcgcgc ccaggtgatc caggtggccc
    33001 cacacctgac ggacgccgag gtggacaccc tggtccagcg tgcggcggcg cgcctgccgg
    33061 cggccgagtc cgaacaggcc gcctatctgc gcgggcaggc gctggcactg gtcaatgagt
    33121 accggctggt gccctgcgtg gtggaggcgc aactcaccag cggcaagccg gcgcacatcg
    33181 aaggcaggct gaacctgacc aacggccaca tctttctgga tgcgtcccat cgccagaccc
    33241 gcctgcaggc gggccagggt gccctgcggg tgagcatcgc cgggacgccg gtggcactgg
    33301 cggcggaatg ctccggccat acgctcaccg gtccggtgct ggacgtggcg gtggcgagcc
    33361 tggtgcctca ccgggacgtg ctcctcgcct gctggcaggc gggctgaggg ccggccgggg
    33421 cgtcagcgtc ccttgaaatc cggcgtgcgt ttggcgaaga aggcgtccag gccttcccgg
    33481 taatcctcgg tggcgagaaa gtcgaaggcg gcgcgctttt cgtccgtgcc gagcgggcgc
    33541 cggtcggcca ggcgatgcac ccactgcttg tgccagcgcg ccaccagcgg cgcgcccttg
    33601 aggatgcggc tgacggtggc gagggcttcc cgctccacct cctcgtcgtc caccacgcgg
    33661 gtcagcaggc ctttggcgag ggcctcgcgg gcgctcagga tgcggccttc caggaggatt
    33721 tccagcagta cggcggggcc gaccagatcc agcaggccgg ccatctcgcc gggatacatg
    33781 gagaaaccga gcttgttgat gggcgcgccg aagcggctcg attcgccggc gatacgcagg
    33841 tcgcagcagc cggcgatctc cagtccgccg ccgatgcagg cgccctggat catcgccacg
    33901 gtgggcaccg ggcaggcgcg gatggcgtcg agggcggcgg cgaccaggtc ttcgtggtaa
    33961 tgcagcgcgt cgtccacggt ggcgcgcacg gtgaggaatt cctcgatgtc gccgccggcc
    34021 gcgaaggcct tgtccccggc gccgcgcagc agcacgcagc gcaggtccgg gtcggcgttg
    34081 agggcggtct gggtggcatg cagggcgcgc cacatgccgg cgtcgacggc gttgagcttg
    34141 tccgggttgg agaggctcag ggtggcgatg ccgccgtccc gctgcaggac gatttctccg
    34201 ctcatgatgg ggtccgtgtt tttttggggt ggtaggccgg cgccgccggc aaaaacgccc
    34261 gtacgttctg gtatggaatc ttgtatatgt tataacattc aacctgggcg tcgcgtcggc
    34321 gcggcttcgc cctgcccgca agggacccta tacataaaaa tgattgcccc ctgtgcccca
    34381 ggcacagacc aacaggcaga tcgcgccaca tccaagtgaa gtttctcgag ggagggaaaa
    34441 atcatgagca ctgcgtctca gagtgcggtc tacgcacgca tccgcagcaa tccccggttc
    34501 gccgagctgg tgcgcaagcg ccagggcttc gcgaccatcc tgtcggtcat cgtgctgacc
    34561 atcttctacg gcttcttcat ggtcgtggcc ttcaacccgc agctcatcgg ccagcgtctg
    34621 tccgaagggt cctacatgac cgtcggcatc gcggccgagc tgttcatgtt catcttcttc
    34681 tggatcctca ccgccgtgta cgtcaagcgc gccaacagcg agttcgacga catcaccact
    34741 gccatcgtga acgacgccgc caaggaggtg aagtgatgat cgcccgtctc aacctggccc
    34801 tgctgggcct gctggccccc gcgctggcct gtgccggcga cgccatctcc gccaccgaga
    34861 agcagcccct caatctgcac gccatcggga tgttcgtgct gttcgtgtcc atgaccctgg
    34921 gcatcaccta ctgggcggcg ggccgcacca agtcggcggc ggatttctac accgctggcg
    34981 gcggcatcac cggcttccag aacggtctgg caatcgccgg tgactacatg tctgccgcga
    35041 ccctgctggg gctcaccgcg ctggtctaca ccaagggcgt ggatggctac atctacatga
    35101 tcgccttctt cgccggctgg cccatcatcc tgttcctgat ggccgagcgc ctgcgcaacc
    35161 tgggcaagtt cacctttgcg gacatcacct cctaccggct caaccagagc aaggtacgga
    35221 ccatggcggc ggtgtcgtcc ctgaccgtgg tgtgcttcta cctggtggcc cagatggtgg
    35281 gtgccggtca gctcatcaag ctgctcttcg gcctggacta caccatcgcc ctgttcgtgg
    35341 tgggcgcgct gatgatggtc tatgtcacct tcggcggcat ggtcgccacc acctgggtgc
    35401 agatcatcaa ggcctgcatg ctgctggcgg ggggcaccct ggtgatggtg ctggccatgt
    35461 cccagttcgg cttcagcttc gagaacctga tgacccaggc cgtctccctg cacagcctag
    35521 gtgagaagat cctgttcccc ggctccctgc tgcccgatcc ggtgactgcc ctgtccctgg
    35581 gcctcggcct gatgttcggc actgccggcc tgccccacat cctgatgcgc ttcttcaccg
    35641 tgaccaacgc caaggaagcc cgcaagtcgg tgctctacgc gtccggtttc gtcgcctact
    35701 tcttcaacgt gatcctgctg atgggcttct ccgccatcat catcgtcggc cagaacccgg
    35761 acttcttcga aggcggcaag atcggcggca agatcatcgg cggcggcaac atggtggcga
    35821 tgcacctggc caaggcggtg ggcggcaacc tgctgctggg cttcctgtcg gcggtggcct
    35881 tcgccaccat cctggcggtg gtgtccggtc tggccctcgc cggtgcggcg gccatctccc
    35941 acgacatcta cgcccgcgtg atccgcggtg gcaaggccac cgagatggaa gagctgcgcg
    36001 tctccaagct ctcttccgtc gccctgggca tcgtggcgat cctgctgggc atcgtcttcg
    36061 agaagatgaa cgtggccttc atggtggcgc tggccttcgg catcgcggcc tcggccaact
    36121 tcccggtgct gatcctctcc atgtactggc gcggcctcac cacccgcggt gcggtggtgg
    36181 gcggctacgt cggcctggtg gcggcggtgc tcttcgtgct gctgtccaag tccgtgtggg
    36241 tgaccgtgct gggcaacgcc caggcgatct tcccctacga ccagccggcc ctgttctcca
    36301 tgcccctggc cttcctggtc accatcgtgc tgtccaagct cgacaacagc gccgaggccc
    36361 aggccgagcg cgctgccttc gaggaccagt acgtgcgggc ccagaccggc tttggtgcgg
    36421 cggaagccag ccagcactga tcgcccctgt tccgggtgcc tgcgggcacc cggtggcagc
    36481 aaaaaccccc ggcgcctcgc ggcgaccggg ggtttttgct tgaatggatc gtgaccgcgt
    36541 caggcggcgg cttcgtcgcg ggtcgaggcg atgcgggcga gggcgcggcg ggcgctggag
    36601 atgtgctcgc gcatggcagc ctcggcgccg gcctcgtcgc gccggtcgag ggcggcgagg
    36661 atcacccgat gctccagcag ggactgctgc aggcggccgt cgatgaacag ggaatggtgg
    36721 cgggacagct tgatcacctt gcgcaggtcc acgatggcct gggtcagcca gcggttgttg
    36781 gcgatgcgct gcagttcctg gtgaaaagcc tggttggcct cgaagaagcc gtcgatgtcg
    36841 ttgctcgccg cggccttttc gagttcgtcg tggatggtgt gcaggcggac cagatcgccg
    36901 tcgccggcac gccgcgcggt gctggcggca cactggccct cgagcagggc catcaccgag
    36961 aagatctcgt ccaggtcccg ctcggagatc tcggtgacgt agcagccgcg gcgcggcttg
    37021 agggtcacca ggccctcgga ggcgaggacc ttgagcgctt cccgcagggg agtgcgggag
    37081 atgccgtact gggcggcgag ggcctgttcg tccacccagg tgccgggcgg cagctcgtgg
    37141 gcaaagatcc gctgacgcag gcgctcggcg acttcctgat agagggcgag gggggcgatg
    37201 cgggtggcgt tcatgggacg gcgggcgtag gagtcggacg gatcaaatcg gctattgcat
    37261 tcataattat ggataaagta ttatcaccaa gggagcaagt caagttcctg tgcaggttgt
    37321 atacccttgg cgacggggcc ccggccgcgg cgcgaaaaac agagggaggg agaaaacggc
    37381 cctgcacggg acacggcagc ccggccgtga ccgttcggtc acggccggtc ctggcgtaca
    37441 acacaatagc ggtcgcgtag cggccgaaca attttatagg gtagaccgcc atggcgaacg
    37501 acagcattcc gaccttcccc caacccgatc tggctgcctg gaacaaggcc gccgccaagt
    37561 cggcccccga aggggatgtc gccaagctca actgggtgac gccggaaggc atcaccgtca
    37621 agccgttgta cacggccgcc gatgtgaacg gtctgtcctt caccgacacc ctgcccggtt
    37681 tcgagccctt cctgcgcggt ccccaggcca ccatgtacgc ggtcaagccg tggaccatcc
    37741 gccagtacgc cggcttctcc accgccgaag cctccaatgc cttctaccgc aaggccctgg
    37801 ccgccggcgg tcagggcgtg tcggtggcct tcgacctggc cacccaccgc ggctacgact
    37861 ccgacaaccc ccgcgtggtg ggtgacgtgg gcaaggccgg cgtggccatc gattccgtcg
    37921 aggacatgaa gatcctcttc aacggcatcc ccctcgacaa gatttccgtc tccatgacca
    37981 tgaacggcgc cgtgctgcc
//
LOCUS       DAOQPA010000021        37597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_452_length_37597_cov_18.906505, whole genome shotgun sequence.
ACCESSION   DAOQPA010000021 DAOQPA010000000
VERSION     DAOQPA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 37597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_37597_cov_18.906505"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..2185
                     /locus_tag="PKX01_04840"
     CDS             275..2185
                     /locus_tag="PKX01_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HMZ75483.1"
                     /translation="MDTPSASDTLIRTQLDFLRRHAPFDRMEPDALRFLGERLSTAYY
                     PAEAEVLMPEDGPPRFFYIVYRGKVQARQVGVTVTEYSTLTLGPGEGFPIGAISAERP
                     STNAYVALEDSYCFQLPAADFLKLLEISPSFQLFCTRYIASLLNQSREQLQVQFAQRA
                     AEQQTMTTSLAQLATKAPVFVGPETPTRSALEKMVEAGVGCLAVAGEDEKPVGVLTQS
                     DILKRIVLPGFDLTRPIGDVMTRPPHTLHATASAYDAALEMATHGVRHILVVDTEDKL
                     KGVISERDLFALQRIGLRQIRSGIESAADIEALQRASRDIRQLALNLLAQGIGAEQLT
                     QFISALNDALTRRIVELNLQRHDLEGIEYAWLAFGSEGRHEQTLSTDQDNGILFTLPE
                     WADKEPLKLRLLAFAKDVNNDLAACGFPLCEGNIMASNPELCLTFDEWREKFGQWIRE
                     PHPEALLNATIFFDYRVLYGDEKLAHKLRQWLLSLTGGNPAFLKSLATNALDVAPPLG
                     KIRDFVVDDDPKYPGTIDLKKFGARLFVDAARVLALRTGVEATATVQRLRQGGSRIGM
                     PAEELSAMADGFHFIQLLRLRHQHLDTDHGAPGDNRVKPDDLNELDRRILKEAFRQAR
                     KLQLRLKLDYQI"
     gene            2182..2820
                     /locus_tag="PKX01_04845"
     CDS             2182..2820
                     /locus_tag="PKX01_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ75484.1"
                     /translation="MNWLTRFLPGGGGTPLTPDQKAALDAVAALPAPDLGRVHFETRY
                     VAVNAETGPVEDGGRRLLAVGAVALNRGLIHPADAYYARLGDTPADALIGLLAFIARA
                     PVVVFNAPFNRGSLERAFEQHLGPLPELAWIDLMVMLPSLYPERIGGQARMDAWLGSF
                     GIDRYDSRDALLESLVVAQLHQVALAATRHFNLPTPRDLLDTESSRRWLRGG"
     gene            complement(2817..3728)
                     /locus_tag="PKX01_04850"
     CDS             complement(2817..3728)
                     /locus_tag="PKX01_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ75485.1"
                     /translation="MLSLRQIRYFQAVADTGSFTLAAAQVYIAQPALSRQIALLEAEL
                     GVALFVREARGVRLTPAGQLFRARTENVGTALEAAVEDARRLDRGEAGVLRLLHSSSV
                     PLAGPLLEALRCLTDAAPAARVELDRVSSEVQVAEIAAGRADVGVARMPVLRREAAVA
                     FTALPEERLVLAVPGAHPLAGRTAVSLAELAGETFVTAVHRERGGLARRVTDLCLARG
                     FVPRSGAVVSRKTSMLNLVAAGYGVAVVPAGMAGLLPAGARLVALTDAEATSASAVLL
                     PLAPSPLARRFAELCGAAWAGVAGAGC"
     gene            3833..4588
                     /locus_tag="PKX01_04855"
     CDS             3833..4588
                     /locus_tag="PKX01_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ75486.1"
                     /translation="MALLPDVPLGDAAGVACAAAILVIAYALFALAGFGSALIASAPL
                     AWVLPVARIVPLLAVLDCAGSVSRAWQARTTVDRAALGRLLPGMLLGQIVGVGLLSAL
                     PAHGLALLLGGFVAGYGVWHALGRRGAEQATPTRAWLYGGFGGVLGGVFGSGGFAYAA
                     YLQPRLERAAFRATQGVLIALSTAWRIALCAASGLVDGRLLVTAALLLPAAWLGLTLG
                     QRIDRRLSGPALGRLIDGLLIVAGLALAGKALA"
     gene            4585..5601
                     /gene="zapE"
                     /locus_tag="PKX01_04860"
     CDS             4585..5601
                     /gene="zapE"
                     /locus_tag="PKX01_04860"
                     /inference="COORDINATES: protein motif:HMM:NF040713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HMZ75487.1"
                     /translation="MTPLRVALEARFRRGGPIPDTAQGAALDHLARLADALVHRRATR
                     GLFLWGGVGRGKTRLMACLDAVLPPAAIRRYPLNALLGAYHATCVCQPDWAARLDTLF
                     GSARVLLLDEFHAYDPADALVLERLLAGVAERGMVCVVTANHPPDRLWPDTPEHRARR
                     HHFAPVAQRLAAQCLLLEVDGGRDYRELLAAHGPLRWWSATDATDRQAARRWLTATAG
                     CARDFDELFCQPRPQRDYVRLAETAGGLLIDGFPRLTPRDGDALRRLVWCLDAAWDAG
                     LALGVLAEAAPETWVAGIGAELDGLLGKDLARARSRLRALCRVDGAGVSAVDLAHEGR
                     RDPR"
     gene            complement(5811..6356)
                     /gene="orn"
                     /locus_tag="PKX01_04865"
     CDS             complement(5811..6356)
                     /gene="orn"
                     /locus_tag="PKX01_04865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HMZ75488.1"
                     /translation="MAQDPNHLIWLDMEMTGLNPDTDRIIEIAMIVTDSQLNLVAEGP
                     VVAVHQPDAVLEAMDDWNKNTHGRSGLIDRVKASTVDEAAAESLFLDFLQAHVPARTS
                     PMCGNSIGQDRRFMVRYMPKLEDFFHYRNLDVSTLKELCKRWRPDVYAGLTKQGAHQA
                     LADIQESIDELRYYRANFLKV"
     gene            6531..7784
                     /locus_tag="PKX01_04870"
     CDS             6531..7784
                     /locus_tag="PKX01_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ75489.1"
                     /translation="MSTSSFTTLFLVAVLAGLAVRIPLALRHARHVRAHRNAVPPAFA
                     TAIGLDAHRKAADYTVARVHLGLVDLLVSTVWLLILTLGGGLQALFDLSAGVLDAGSL
                     GQGVAFLTLVAVASWLVDLPLGLYRSFVLEARFGFNRLTPRLFVADTLKGALLAALIG
                     LPLLAGALWLMGAMGEHWWLWVWAAWLGFNLLAMLIYPSFIAPLFNRFQPLEDGDLKT
                     RIEALMTRCGFRLSGLFVMDGSKRSAHGNAYFTGFGGSRRIVFFDTLLEKLDPAEVEA
                     VLAHELGHYHHHHLWKRLAVIGTGSLAFFALLGALAQQGWFFTGLGMTATGAAPTLVL
                     FSLALPVFTFPLTPLMSHWSRRHEFEADAYAARQAPAASLISALVKLYRDNASTLTPD
                     PLYSRVYDSHPPAAVRIARLEAAVR"
     gene            7781..8674
                     /gene="rsgA"
                     /locus_tag="PKX01_04875"
     CDS             7781..8674
                     /gene="rsgA"
                     /locus_tag="PKX01_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ75490.1"
                     /translation="MKARGTLVAAFGRQYEVRTDGGEILLCVPRGKKSRFACGDRVQV
                     ARSAPGQGVIEQLEERGSLLYRSDAWRQKLIAANATQVVVVVATEPSFSDDFVTRCIC
                     AAESQQLKSLIVLNKADLPAGLDAARTLLAPFAALDYPILELSALHDVDALRARLKDE
                     TSLLVGQSGMGKSTLTNALIPEARAETREISEVLDTGKHTTTFARLYDLPGGGGLIDS
                     PGMQTFGLAHLDNAALEASFREFRPWDGQCRFRDCRHDSEPGCALRAAVADGRIAPRR
                     LTQFLELRAECEQARKTAQGW"
     gene            complement(8857..9234)
                     /locus_tag="PKX01_04880"
     CDS             complement(8857..9234)
                     /locus_tag="PKX01_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ75491.1"
                     /translation="MSQSEVTEFVELPPIPAKRYFTIGEVSELCAVKPHVLRYWEQEF
                     TQLRPVKRGGNRRYYQHHEVLLIRRIRELLYRDGFTISGARNRLDEMASRKREDVEES
                     AVLRDQLASIRGEIQAILKDLQG"
     gene            complement(9221..9526)
                     /locus_tag="PKX01_04885"
     CDS             complement(9221..9526)
                     /locus_tag="PKX01_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HMZ75492.1"
                     /translation="MGVTLTKAELADMLFEQVGLNKREAKDMVECFFEEIRLALERGD
                     CVKLSGFGNFQLREKPQRPGRNPKTGEEIPITARRVVTFHASQKLKANVEHLSDVAK"
     gene            complement(9532..11910)
                     /gene="pheT"
                     /locus_tag="PKX01_04890"
     CDS             complement(9532..11910)
                     /gene="pheT"
                     /locus_tag="PKX01_04890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ75493.1"
                     /translation="MQFSELWLRSFVSPALDADALGHLLTMAGLEVEEQGGVAAAFSK
                     VVVARILEADKHPNADKLRVCKVDAGQGEPLQIVCGAPNAAAGLTIPCALVGAELPGF
                     AIKAAKLRGVESFGMLCSAKELGIADDSSGLLVLPEDAPVGTDLRDYLALDDTVYTIK
                     LTPNRADCLSLTGIAREVAALTDRPLSLVDTSSVAPTIADTRKVVLDAPEACPRYCGR
                     VVRGVNAQAPTPDWMKQRLARSGIRSISVLVDVTNYVMLELGQPLHAFDNARLDGAIH
                     VRMPKAGEQVLLLNGQTVTPAADTLLIADESRALALAGIMGGEESGITLETTDLFLES
                     AFFAPDAIAGRARAYGFSSDASYRFERGVDFALAPAALERATRLILDLCGGEAGPVVE
                     ALAADRLPQRAPIRLRAARVRRVLGIDVSADEVADLFGRLNLAVNRDGDDFVVTPPSY
                     RFDLEIEEDLIEEIARLHGYDNIPTVVPKGPIAMLPRPEARRDPMGVRRLVAAREYQE
                     VVNFSFVEEAWERDFCGNADPVRLANPIASQMSVMRSSLIGGLVANVVTNRKRQNNRV
                     RVFELGRIFRRDAAGAPVEGFHQPVRVAALAAGPLRPEQWGEAVRTVDFFDVKADLEA
                     LLHPRVATFEPVVHPALHPGRAARVLLEGQEIGVIGELHPVWVQRYELGTAPVVFEVS
                     LTSLLETMTPAYREVSRFPAVTRDLALVVDQATPVASLIAGLSSVAPAIVQRIELFDQ
                     YQGKGIEPNRKSLAFRVVMQETQRTLEDAEVEAAMTALTRHAEEALGAKLRG"
     gene            complement(11914..12945)
                     /gene="pheS"
                     /locus_tag="PKX01_04895"
     CDS             complement(11914..12945)
                     /gene="pheS"
                     /locus_tag="PKX01_04895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMZ75494.1"
                     /translation="MDQLDQLVAQAGADFAAAADSAALETAKARFLGKGGSITELLKG
                     LGKLDAEARKAAGAEINKAKSAIEAALEARREALRQAALEAQLAAEALDVTLPGRGAG
                     LGGLHPVVRTLERVEQLFRSIGFEVADGPEIETDFYNFTALNTPANHPARSMHDTFYL
                     ENGEGVLLRTHTSPVQIRAMQAHVDAYKDRDGMPELRVIAPGRVYRVDSDATHSPMFH
                     QVEGLWVGESVSFADLKGVIADFLRRFFESDDLQVRFRPSFFPFTEPSAEIDVAFMSG
                     ALKGRWLEIAGCGMVHPNVLRFGGIDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEG
                     DLRFLGQFR"
     gene            complement(13016..13375)
                     /gene="rplT"
                     /locus_tag="PKX01_04900"
     CDS             complement(13016..13375)
                     /gene="rplT"
                     /locus_tag="PKX01_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMZ75495.1"
                     /translation="MPRVKRGVTARARHKKVLDQAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRALWIARINAASRELGLTYSRFMNGLKKAAIEVDRKVLADLAVFD
                     KPAFAALAEQAKAKLAA"
     gene            complement(13390..13587)
                     /gene="rpmI"
                     /locus_tag="PKX01_04905"
     CDS             complement(13390..13587)
                     /gene="rpmI"
                     /locus_tag="PKX01_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMZ75496.1"
                     /translation="MPKMKTKSGAAKRFKVRSSGSIKRSQAFKRHILTKKTTKSKRHL
                     RGMTCVHASDEKSIRAMMPYA"
     gene            complement(13730..14233)
                     /gene="infC"
                     /locus_tag="PKX01_04910"
     CDS             complement(13730..14233)
                     /gene="infC"
                     /locus_tag="PKX01_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMZ75497.1"
                     /translation="MRINGEITAPEVRLTGPDGEQIGIVSLRAALEAADEAGVDLVEI
                     APMAKPPVCRLMDFGKYKYEEAKRAHEAKQKQKQIQVKEVKLRPGTDENDYQIKLRNL
                     IKFLNEGDKAKVTLRFRGREMAHQEFGMRQLERIKADLEEIAQVEQMPKMEGRQMIMI
                     IAPKRKH"
     gene            complement(14280..16196)
                     /gene="thrS"
                     /locus_tag="PKX01_04915"
     CDS             complement(14280..16196)
                     /gene="thrS"
                     /locus_tag="PKX01_04915"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMZ75498.1"
                     /translation="MPNITLPDGSVRSFDQPVTIAQVAASIGAGLARAALAGKVDGRL
                     VDTSFLIDRDVQLAIVTDKDADGLELIRHSTAHLLAYAVKALFPDAQVTIGPVIENGF
                     YYDFSYTRAFTPEDLAKIEARMLELAKKDIPVERSVLPRDEAVAYFKSIGEHYKAEII
                     ASIPANEDVSLYKEGDFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSRNEMLQRIY
                     GTAWAKKEDQDAYLHMLEEAEKRDHRRLGRQLDLFHLQDEAPGMVFWHPHGWTLWQQV
                     EQYMRSVLNEAGYQEVKTPLVMDRALWEKSGHWDNYRDNMFTTESEKRDYAIKPMNCP
                     GHVQIYNHGLHSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEEQI
                     VSEVTAFNELLTRVYRDFGFTDVAVKLSLRPEKRAGTDAIWDKAETGLRQALAASGHS
                     WEELPGEGAFYGPKIEYHIKDALGRSWQCGTMQLDFVLPERLGAEYVAEDNARYRPVM
                     LHRAILGSLERFIGILIENHAGALPLWLSPVQAVVMNISEAQADYVTEVVNRLRSRGF
                     RVEADLRNEKITYKIREHSVRKLPYLLVIGDKEKAADVVAVRARGNQDLGQMSLEQVV
                     ERWQRELLARAGTA"
     gene            complement(16334..16410)
                     /locus_tag="PKX01_04920"
     tRNA            complement(16334..16410)
                     /locus_tag="PKX01_04920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16374..16376),aa:Val,seq:gac)
     gene            complement(16471..17268)
                     /locus_tag="PKX01_04925"
     CDS             complement(16471..17268)
                     /locus_tag="PKX01_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ75499.1"
                     /translation="MVPILVFDIETIPDVAGIRALNGLPAELGDDEVAEFAFQQRRAA
                     TGNDFLPHHLQRVVTISCVLRDDSQFRVFSLSEPDATEGQIIQRFFDGVEKFTPQLVS
                     WNGGGFDLPVLHYRGMMHGVAASRYWDLGDGDYFDSRDFKWNNYIGRYHTRHLDLMDL
                     LALYQPRASAPLDDLAKLMGFPGKLGMDGSAVWQGYRDGRIAEIRDYCETDVVNTYLV
                     LLRFQRMRGILGAERYEEEIAFVRKSLSSLPARHWQRFLEAWGDGSS"
     gene            complement(17275..18165)
                     /gene="cysM"
                     /locus_tag="PKX01_04930"
     CDS             complement(17275..18165)
                     /gene="cysM"
                     /locus_tag="PKX01_04930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HMZ75500.1"
                     /translation="MEFKTLEDYVGQTPLVRLKRITAGRNNTILAKLEGNNPAGSVKD
                     RPALSMILHAEARGDIRPGQTLIEATSGNTGIALAMAAAMRGYGMILVMPENQSIERR
                     QTMRAFGAELVLTPKEGGMELARDVAERMRDEGRGVILDQFANPDNPLAHIEGTGPEI
                     WDQTGGTVTHFVSSMGTTGTIMGVSTYLKARNPAIRIIGCQPADGAQIPGIRKWPEAY
                     LPRIYDKARVDQLEYVAQADAEEMTRRLAREEGIFAGISSGGALHVALRLAAQLEDAV
                     IVSIVCDRGDRYLSTGVFPA"
     gene            complement(18368..19543)
                     /gene="lapB"
                     /locus_tag="PKX01_04935"
     CDS             complement(18368..19543)
                     /gene="lapB"
                     /locus_tag="PKX01_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HMZ75501.1"
                     /translation="MEFELWWLLAFPLFFALGWLAARIDIKHLVKESRALPRTYLTGL
                     NFLLNEQPDKAIDAFIEAVKIDNQTVELHFALGSLFRRRGETDRAIRMHQNLIDRDDL
                     SQDQRLQALSELGLDYLKAGLLDRAEAVFVQLRGSRLDEFALKNLLDIYQQEKDWRKA
                     IDIAQALPGHESALWEKEVANFHCELAAAAIADSRFDEARSNLERALETNRKSVRASI
                     LLGDLHAAQGDDEAALEAWKRVETQNPVYLALVAERIMDAYRRLGRLEQGMQLLRSYL
                     DSYASLDLLDAVFQWELQKEGAQAAYHLVREELRRNPTLLGLDKLLEAALLTAPVEQR
                     TDIELVKGLVHGHTRRVARYRCDACGFKARQFYWRCPACGGWETYPPRRTEEFDLTP"
     gene            complement(19550..19855)
                     /locus_tag="PKX01_04940"
     CDS             complement(19550..19855)
                     /locus_tag="PKX01_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HMZ75502.1"
                     /translation="MRLLTWIVRLFLFLVLFGFAVKNDDVVTLRFFFGAQWQVPLVLV
                     ILLFVVIGVLIGVTAAVATLYRQHRELGQLRERQADAPPPVTVRPPVAHGPDLPETY"
     gene            complement(19948..20232)
                     /locus_tag="PKX01_04945"
     CDS             complement(19948..20232)
                     /locus_tag="PKX01_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HMZ75503.1"
                     /translation="MTKSELIARLAERFPQLVAKDAETAVKMILDAMTDALSRGDRIE
                     IRGFGSFALNYRPPRVGRNPKSGEKVQVPEKYVPHFKAGKELRERVDMIV"
     gene            complement(20244..21950)
                     /gene="rpsA"
                     /locus_tag="PKX01_04950"
     CDS             complement(20244..21950)
                     /gene="rpsA"
                     /locus_tag="PKX01_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMZ75504.1"
                     /translation="MSIATPVSIEESFAALFEESLNRQEMRAGEVITAEVVRIDQNFV
                     VVNAGLKSESYIPLDEFRNDRGELTANVGDYVQVAIEQLEDGYGETRLSRDKAKRIAA
                     WNDLDKALNEGILVKGVITGKVKGGLTVMVNGIRAFLPGSLVDMRPVKDTTPFEGKEF
                     EFKVIKLDRKRNNVVVSRRAVLEASMGEERQKLLETLKEGTVVKGVVKNITDYGAFVD
                     LGGIDGLLHITDLAWRRVRHPSEVLSVGDELEAKVLKFDQEKNRVSLGLKQLGEDPWV
                     GISRRYPQGTRLFGKVTNITDYGAFVEVEQGIEGLVHVSEMDWTNKNIHPTKVVQLGD
                     EVEVMILEIDEDRRRISLGMKQCMSNPWDDFAINHKKGDKVRGQIKSLTDFGVFIGLE
                     GGIDGLVHLSDLSWSLAGEEAVRQYKKGDEVEAVVLNIDVEKERISLGIKQLEGDPFT
                     NFIATHDKNTLVTGTVKSVDARGAVVALNDDVEGYLRASEFSRDRVDDLSQLLKVGDA
                     VEALIVNVDRKTRGINLSVRAKDQVEQAEAMNKLASDSGSAASGTTNLGALLKAKLNE
                     QK"
     gene            complement(22046..24007)
                     /locus_tag="PKX01_04955"
     CDS             complement(22046..24007)
                     /locus_tag="PKX01_04955"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HMZ75505.1"
                     /translation="MEFIDLPPMLRAVGEVRLPGSKSISNRVLLLAALAEGTTRVEAL
                     LDSDDTRVMRDALVALGVGLQQEGDTLVVTGCGGRFPNARADLFVGNSGLSIRTLVPA
                     VVASLAGCGDADAAVRLSGVPRMHERPIGDLVDGLAQLGARVDWLGQPGFPPLALRPA
                     PLAAETVSVRGNVSSQFLTGLLQAAPLVARDTPLVIVVEGELISRPYVEITLNLMARF
                     GVQVERDGWNRFVVLAGQRYVSPGRIAVEGDASTASYFLAAGALGGGPLRVKGAGRGS
                     IQGDVRFADALAEMGAQITWGEDWIEARAPESGRLKGIDLDLNHIPDAAMTLAVAALV
                     ADGPSHLRNIGSWRVKETDRIAAMATELRKLGATVEEFPESLRITPPARLQPAVIDTY
                     EDHRVAMCFSLATLCGTSLRINDPKCVNKTFPGYFETFAGITAPVPVVAIDGPSASGK
                     GTVAARVAQALGFAHLDSGSLYRIVALTAIRRGVALDDEPTLAAIATDLPARFEGDRV
                     MLEGDDVTEAIRSEACSLGASRVAALPTVREALFWRQRAYRRAPGLVGEGRDMGSVVF
                     PDAPVKIFLTASVEARAERRYKQLMDKGMPANMADLLKDLRERDARDAQRAAAPLKQE
                     EDAVLLDTSDMSVEQAVAFVLERAAILKG"
     gene            complement(24022..24915)
                     /locus_tag="PKX01_04960"
     CDS             complement(24022..24915)
                     /locus_tag="PKX01_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HMZ75506.1"
                     /translation="MRKIDKLVICGVGLIGGSVALALRQARLVNRIVGVGRRRETLEQ
                     ALQLGVIDEISTDWAEALDGAEIVLLAMPVGQTDAVAAAMAPHLGPDTLVTDAGSTKR
                     DVIEAIYRHLAAHLPRVVPAHPIAGAEKSGPSAARADLYRKRKVVVTPLPENDAVAVA
                     RVRELWAACGAHLFEMTPQEHDRVFAAVSHLPHLLAFGLVHDLAGRANAEELFSYAAS
                     GFRDFTRVAGSHPEMWRDICLANRHSLLAELDAYLGELAWLRALLASGDGPALEKVFS
                     EASRARNEWADKAFPATPSGE"
     gene            complement(24926..26023)
                     /gene="hisC"
                     /locus_tag="PKX01_04965"
     CDS             complement(24926..26023)
                     /gene="hisC"
                     /locus_tag="PKX01_04965"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMZ75507.1"
                     /translation="MRLCDLSPAHIRAISPYQPGKPIAELAREMGLAESSIVKLASNE
                     NPLGFGDKARAAMAAALDDLARYPDGNGFELKQALCRRYGVAMDEIVLGNGSNDVLEL
                     AARAFLGPQTSAIYAQHAFAVYPLATQAVGARGVEVPARHFGHDLDAMLAAITPDTRV
                     LFIANPNNPTGTFVPGPELEAFLVRVPDHVLVVLDEAYTEYLGEEQRYDAIAWLARFP
                     NLLVCRTFSKAYGLAGLRVGYGLGHPAVIDLLNRVRQPFNVGSVALAAAAAALFDSDF
                     LARSYEVNRAGMAQLTAGFARLGLEWIPSAGNFVTFRAGDGAGVNRRLLRQGVIVRPI
                     AGYGMPEWLRVSIGLEAENARFLEALPQALG"
     gene            complement(26040..27119)
                     /gene="pheA"
                     /locus_tag="PKX01_04970"
     CDS             complement(26040..27119)
                     /gene="pheA"
                     /locus_tag="PKX01_04970"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HMZ75508.1"
                     /translation="MLQELGAVRDQIDALDAQMLALLNARARCAQHVGEIKARHGEAG
                     QVYRPEREAQVLRRIQALNAGPLPDESVTFFFREVMSACLSLENPLGVAFLGPLGTFS
                     ESAAVKHFGHAARLAPQTTIDDVFREVESGHAQYGVAPVENSTEGAVGRTLDLLLSSP
                     LQICGEVVLRIHQHLMSGETALGDVRKVYSHAQSLAQCHEWLNSHLPGVPRISVASNA
                     EAARLAASEPGAAAIAGEAAAERYALPTLARSIEDEPNNTTRFLVLGRHDAGPSGADK
                     TSLIMSAANRPGAMHALLAPLADAGVSMTRLESRPARAALWEYVFYVDFAGHRNDPEV
                     RKALDALSAHASFLKILGSYPQAVY"
     gene            complement(27155..28240)
                     /gene="serC"
                     /locus_tag="PKX01_04975"
     CDS             complement(27155..28240)
                     /gene="serC"
                     /locus_tag="PKX01_04975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMZ75509.1"
                     /translation="MRIYNFSAGPAALPTPVLEQVREELLDWHGAGMGIMEMSHRGKD
                     FMSVIAAAEADLRALMGIPDGYKVLFLQGGATQMFAQIPMNLLAGRSADYVVTGAWSK
                     KAWKEAPHIGPVRLAGTTEADGRFTRLPAADELSFDPDAAYVHLCTNETIHGVEMFDL
                     PATPAGVPLVADMSSHILSRPMDVSRYGLIYAGAQKNIGPAGLVLVIVREDLLGRASA
                     ATPSVMNFATMAEHGSMLNTPPTFGIYLAGLIFKDLLRQGGLAAVEAANRAKAERLYD
                     AIDGSGGFYRNPVDRPCRSLMNVPFTLADPALEADFLAEAAARRLAGLKGHKSVGGMR
                     ASIYNAMTLDGVEALVDLMQDFARRKA"
     gene            complement(28268..30841)
                     /gene="gyrA"
                     /locus_tag="PKX01_04980"
     CDS             complement(28268..30841)
                     /gene="gyrA"
                     /locus_tag="PKX01_04980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMZ75510.1"
                     /translation="MIPFAKETLPISLEDEMRHAYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGNDWNKAYKKSARIVGDVIGKYHPHGDQSVYDTIVRMAQDFSLRYML
                     VDGQGNFGSVDGDGAAAMRYTEIRMAKIGHELLADIDKETVDFGPNYDGSEKEPLVLP
                     ARIPNLLINGSGGIAVGMATNIPPHNLGEVVDACLRLLQEPDVSIDELIKIVPAPDFP
                     TAALIYGLSGVHEGYRTGRGRVVMRARTHFEDIGKGDRQAIIVDELPYQVNKKALLEK
                     IAELVNDKRIEGISEIRDESDKSGMRVVIELKRNEMPDVVLNNLFKQTQLQDTFGMNM
                     VALVDGKPRLLDLRQMLECFLAHRREVITRRTVFELRKARERGHILEGLAVALSNVDE
                     IIALIKAAPTPPEAKKSLMARQWRSPLVEEMLARAASDSFRPTGLAPEFGLSAQGYRL
                     SDAQAQAILELRLQRLTGLEQDKIVAEYKEVMETIADLLDILARPERITAIIGDELAL
                     IKQQFGDPRRSEVVLNTADINIEDLIAPQDMVVTLSHTGYFKRQPLTDYRAQKRGGRG
                     KQAAAVKEDDFVDQLFVANTHDHILCFSNRGRLYWLKVYEVPEGNRNARGKPIVNLFP
                     LLEGEKITAVLPVKEFDEGHFVFMATAQGTVKKTPLTDFSNPRKAGIIAVALDEGDYL
                     VGVAITDGQHDVMLFSDAGKAVRFSENDVRPMGRTARGVRGMMLEDDQSVIALLVAGD
                     ESPSVLTATENGYGKRTPIAEYTRHGRGTKGMIAIQTSDRNGKVVAATLVTEKEQIML
                     ITTAGVLVRTRVSEIREMGRATQGVTLISVDEGSTLSGVQKIVESEEDEQDGEGPRAD
                     A"
     gene            31072..31800
                     /locus_tag="PKX01_04985"
     CDS             31072..31800
                     /locus_tag="PKX01_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ75511.1"
                     /translation="MNTAFRVASATLLALTGLTAQAQSKDIVVDPKGDVPYAIDQRNV
                     VVKSGADLCWRTGSWTPAAAGSVQAGELPVGCQCDRDVVGADICQPPAARGASGAPAA
                     AVAAPAAAVAAPRTCDFSVTLNADQSFAFGKATLSPAARRALDEVATRSAQCASVRAL
                     LITGHTDRLGTQAYNQKLSEKRAGAVSSYLAGKGLKAGDVEIMGAGKTQPIKGCDDSL
                     GHKKLVDCLAPNRRVTIDVKGPAK"
     gene            31872..33203
                     /locus_tag="PKX01_04990"
     CDS             31872..33203
                     /locus_tag="PKX01_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HMZ75512.1"
                     /translation="MAPVETVADIDLLIEARWIIPVQPAGLTLERHAVAIHEGRILAV
                     LPIDNARRQFRPREIRPLTDHILMPGLVNLHVHAAMTLMRGLADDLPLMRWLQEAIWP
                     AEGRHVSPGFVRDGSLLAAAEMLRGGITTCNDMYFFPEAAAEAFSRVGLRAMLGITVL
                     DFPTQYAADADDYLRKGLAARDAWRHDPLIGFTLAPHAPYTVSDRTFERVGGLAEELD
                     LPIHIHLHETATEVADSLRDHGLRPIARLDRLGLLGPGLIGVHAVHLSPEDIDRLATH
                     HCSIAHCPTSNMKLASGAAPMAAVAERGLRIGLGSDGTASNNRLDLFREMHQAALLAK
                     LTSGDAGALPAHRVLRMATLDGAAALGLDDRIGSIEPGKAADLCAVRLDDVDTLPCYD
                     PASHLIYVLGRESVSDVWVAGRQALKDGTLLQISNTELSSLARLWQNRLRQ"
     gene            33295..34026
                     /locus_tag="PKX01_04995"
     CDS             33295..34026
                     /locus_tag="PKX01_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ75513.1"
                     /translation="MIKQAKKQLLMAAALATLGLTASASFAKDVVVDTKGEVPYVIDG
                     RNVVARSGADLCWRTGYWTPAAASTTMAGEFPVGCECDKDVVAKEKCEPKAAAPAAGA
                     KGAAPIAAPAPVSEKVKLAADTLFDFDKAVLLPAGKTKLDDLADKAKGLNLEVILAVG
                     HTDRIGGDAYNQKLSEKRAAAVKDYLVSKGVAANRVYTEGKGKKQPVTGDKCKGLGKD
                     NGKNKKLVECLQPDRRVEIEVIGTK"
     gene            34157..34852
                     /gene="ubiG"
                     /locus_tag="PKX01_05000"
     CDS             34157..34852
                     /gene="ubiG"
                     /locus_tag="PKX01_05000"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HMZ75514.1"
                     /translation="MNADPAELQKFSELAHRWWDPESEFKPLHQINPLRLDWIDETVH
                     LAGKQVIDVGCGGGILSESMAARGAHVTGIDLSDKALGVARLHLYESGQTVDYRHISA
                     EDMARETPGQFDAVTCMEMLEHVPDPASTIRACTALVKPGGHVFFSTLNRNPKAYLMA
                     VVGAEYVLKLLPKGTHDYAKFIKPSELSRYVRNAGLDVEELIGMRYNPFTQIYALGRD
                     TDVNYLMRTRRNG"
     gene            34845..35492
                     /gene="gph"
                     /locus_tag="PKX01_05005"
     CDS             34845..35492
                     /gene="gph"
                     /locus_tag="PKX01_05005"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237005.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HMZ75515.1"
                     /translation="MADAVFFDLDGTLADTAPDLAVALNQLRAELGLPPCRFESLRPF
                     VSQGTRGMLSAGLGILPDHAEYAPLFDRFLSLYAGNLCVHTRLFTGMEVVLDALDERG
                     LPWGVVTNKAERFARPIITALGLDGRCACVVGGDTLTRRKPFPDPLLHAADLARVAPT
                     RSLYVGDDPRDITAGRAAGMQTVGARYGYLGSNPAIDTWGADALISHPEELLAHL"
     gene            35548..35912
                     /gene="ssrA"
                     /locus_tag="PKX01_05010"
     tmRNA           35548..35912
                     /gene="ssrA"
                     /locus_tag="PKX01_05010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            36076..37311
                     /locus_tag="PKX01_05015"
     CDS             36076..37311
                     /locus_tag="PKX01_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ75516.1"
                     /translation="MALTDTAIKSAKPTDKPVKMTDGDGLYLLIHPNGGKWWRFDYRF
                     DSKRKTLSMGVYPDVSLRDARNRRDAARKQIANGIDPGEHRKAHKSARADAVANSFEA
                     VAREWYSKMRPTWTPDHADKIIKRFERDVFPWIGGKPVADLNAPEVLTTIRRIEARGR
                     LETAHRALQSCGQVFRYAVATGRAERDPTGDLRGALPPTREKHMAALTDPAEVGALLR
                     AVDAFRGTLIVQSALNLSALVFVRPGELRQAEWSEIDLDKGEWNIPASRMKGWTRKGI
                     TTPHLVPLAPQAVAILRELQPLTARGAHVFAGRDPKKPMSNAAVNAALRRMGYDTKTE
                     MTGHGFRAMARTILHEELGVDRDVIEHQLAHAVPDALGTAYNRTKFIKERRVMMTRWA
                     DYLDKLKVGAEVVTMRGAA"
BASE COUNT         6374 a        12847 c        11968 g         6408 t
ORIGIN      
        1 ccaggtcgaa ctcgtccacc accggttcga agtcgtgccc atcggcgccc aggccgtgga
       61 gccagatcac cgaccgggtt gccggcacag ccggttcaat ctcgacaaca gggagcaggg
      121 cggcgttcat gggcgatgtc cggagaaggg gagcccgcat tctagccgag ggcctccttt
      181 tgcctcgaaa ccacgcggaa tgttgaggaa aatcatgacg ggagggcagc ggaagagtag
      241 attcatgggt ttgatcgcgt cagtgatggc cgttatggat actccctccg cttccgatac
      301 cctgatccgc acccagctgg atttcctgcg tcgccatgcg cccttcgacc gcatggagcc
      361 agacgccctg cgcttcctcg gcgaacggct gtccaccgcc tactacccgg ccgaggcgga
      421 agtcctgatg cccgaggacg gcccaccgcg cttcttctac atcgtctatc gcggcaaggt
      481 acaggcccgc caggtcggcg tcaccgtcac cgagtattcc accctcaccc tgggtcctgg
      541 cgaaggcttc ccgattggtg cgatttcggc ggagcgcccg tccaccaacg cctacgtggc
      601 cctggaagac agctactgct tccagctccc cgccgccgac ttcctcaagt tgctggagat
      661 cagcccgagc ttccagctct tctgcacccg ctacatcgcc agcctgctca accagtcgcg
      721 ggagcagctc caggtccagt tcgcccagcg tgcggccgaa cagcagacca tgaccacctc
      781 cctggcccag ctcgccacca aggcgccggt cttcgtcggg cccgagacgc ccacccgcag
      841 cgccctcgaa aagatggtcg aggccggtgt cggctgtctg gcggtggctg gcgaggacga
      901 aaaacccgtc ggcgtgctca cccagagcga catcctcaag cgcatcgtgc tgcccggctt
      961 cgacctcacc cgccccatcg gcgacgtgat gacccgcccg ccccacaccc tccacgccac
     1021 cgccagcgcc tacgacgccg ccctcgagat ggccacccac ggcgtgcgcc acatcctcgt
     1081 ggtggatacc gaggacaagc tcaagggcgt gatctccgag cgcgacctct tcgccctgca
     1141 gcgcatcggc ctgcgccaga tccgctccgg tatcgaaagc gcggccgaca tcgaggccct
     1201 gcagcgcgcc agccgcgaca tccgtcagct cgccctcaac ctgctggccc agggtatcgg
     1261 cgccgagcag ctcacccagt tcatctcggc cctcaacgac gccctcaccc gccgcatcgt
     1321 cgagctcaac ctgcagcgcc acgacctgga aggcatcgaa tacgcgtggc tcgccttcgg
     1381 ctccgagggc cgccacgaac agaccctgtc caccgaccag gacaatggga tccttttcac
     1441 gctgcccgaa tgggccgaca aggaacccct caagctgcgc ctgctggcct tcgccaagga
     1501 cgtgaacaac gacctggccg cctgcggctt ccccctgtgc gagggcaaca tcatggccag
     1561 caacccggag ctgtgcctga ccttcgacga atggcgcgag aagttcggcc agtggatccg
     1621 cgaaccccac cccgaagccc tgctcaacgc caccatcttc ttcgactacc gcgtgctcta
     1681 cggcgacgaa aagctcgccc acaagctgcg ccagtggctg ctcagcctca ccggcggcaa
     1741 tccggccttc ctcaagtcgc tggccaccaa cgccctcgac gtggcgccgc ctctgggcaa
     1801 gatccgcgat ttcgtggtgg atgacgatcc caaatatccg ggcaccatcg acctcaagaa
     1861 attcggcgcc cgcctcttcg tggatgccgc ccgcgtgctg gccctgcgca ccggcgtcga
     1921 ggccaccgcc acggtccagc gcctgcgcca gggcggcagc cgcatcggca tgcccgccga
     1981 agaactctcc gcgatggccg acggcttcca cttcatccag ctcctgcgcc tgcgccacca
     2041 gcatctcgac accgaccacg gcgcgcccgg cgacaaccgg gtcaagccgg acgacctcaa
     2101 cgagctcgac cgccgcatcc tgaaggaagc cttccgccag gcccgcaaac tgcaactgcg
     2161 cctcaagctg gactaccaga tatgaactgg ctcacccgat tcctgccggg cggcggcgga
     2221 acgcccctca cgcccgatca gaaggccgcc ctcgacgcgg tggccgccct gcctgcaccg
     2281 gatcttggcc gcgtccattt cgagacccgc tacgtggccg tcaacgcaga gaccggcccg
     2341 gtcgaggatg gcggacggcg cctgctggcg gtaggcgccg tggccctcaa ccgcggcctg
     2401 atccatccgg cggacgccta ctacgcccgc ctcggcgaca cacccgccga cgccctcatc
     2461 gggctcctcg ccttcatcgc ccgcgcgccg gtggtggtct tcaacgcccc cttcaaccgg
     2521 ggcagtctgg agcgcgcctt cgagcagcac ctgggcccgc tgcccgagct cgcgtggatc
     2581 gacctgatgg tcatgctgcc cagcctctac cccgaacgca tcggcggcca ggcgcgcatg
     2641 gacgcctggc tcggcagctt cggcatcgac cgctacgaca gccgcgacgc cctcctcgaa
     2701 agcctggtcg tcgcccagct ccatcaggtg gccctggccg ccacccggca tttcaacctg
     2761 cccacccccc gcgacctgct cgacaccgag agcagccgcc gctggctgcg gggcggctag
     2821 cagcccgccc cggccactcc ggcccacgcc gccccgcaca gttcggcaaa gcgccgcgcc
     2881 agcggggacg gcgccagagg caacagcacg gccgacgcgg acgtggcctc cgcgtccgtc
     2941 agcgccacca gccgggcacc ggccggcagc agcccggcca tgcccgccgg caccaccgcc
     3001 accccgtagc cggccgccac caggttgagc atggacgtct tgcgggacac caccgcaccg
     3061 ctgcgcggca cgaagccccg tgccaggcac aggtccgtca cccgccgggc cagtccgccc
     3121 cgctcccgat ggaccgccgt cacgaaggtc tcgccagcca gctcggcgag actcaccgcc
     3181 gtccgcccgg ccagcggatg agcccccggc accgccagca ccagccgctc ctccggcagc
     3241 gccgtgaacg ccactgcggc ctcccggcgc agcaccggca tccgcgccac ccccacgtcc
     3301 gcccgcccgg cggcgatctc cgccacctgc acctcggagg acacccggtc cagctccacg
     3361 cgggccgccg gcgccgcgtc ggtcaagcat cgcaaggcct ccagcaaggg gccggccagg
     3421 ggcacagaac tggaatgaag caggcgcaac acaccggcct cgccccggtc gaggcgccgt
     3481 gcgtcctcca ccgccgcctc cagcgccgtc ccgacgttct ccgtgcgggc ccggaacagc
     3541 tgtccggccg gggtcagccg cacgccgcgc gcttcccgta cgaagagcgc cacgcccagt
     3601 tccgcttcga gcaaggcgat ctgccggctg agcgcaggct gggcgatgta cacctgcgcc
     3661 gccgccagcg tgaagctgcc ggtgtcggcc acggcctgga aatagcggat ctggcgcaga
     3721 ctaagcatcg caaaaaggca tggataagca ggatttttga tattggcgtt atacgtctgc
     3781 cgggccaaca ataccagcat cgcaacacct ggcctcaccc gtgcctccgc tcatggccct
     3841 gctccccgat gtgccccttg gcgacgccgc cggagtggcc tgcgccgcgg cgatcctggt
     3901 catcgcctac gccctctttg ctctggctgg cttcggctcg gcgctgatcg ccagcgcgcc
     3961 gctggcctgg gtactgccgg tggcacgcat cgtgcccctg ctggcggtgc tcgattgcgc
     4021 cggttcggtg agccgggcgt ggcaggcacg tacgaccgtc gatcgggcgg ccctcgggcg
     4081 cctgctgccc ggcatgctgc tcggacagat cgtcggtgtc ggactgctga gcgccctgcc
     4141 cgcccacggg ttggccctgc tcctgggagg ctttgtagcc ggctacggcg tctggcatgc
     4201 gctcggtcgc cgcggcgcgg agcaggccac ccccacccgg gcatggctgt atggcggctt
     4261 tggcggcgta ctgggcggag tatttggcag cggcggcttc gcctacgcag cctacctgca
     4321 gccgcgcctc gagcgggcgg ccttccgtgc cacccagggc gtgctgatcg ccctgtccac
     4381 cgcctggcgc atcgccctgt gtgcagcctc cgggctcgtg gatggccgcc tgctcgtcac
     4441 cgccgccctg ctcctgcccg ccgcctggct gggcctgacc ctgggtcagc ggatcgaccg
     4501 ccgcctctcc ggccccgccc tcggacgcct gatcgacggc cttctcatcg tcgccggcct
     4561 cgccctggcg ggcaaggcgc tggcatgacg cccctgcgtg tcgccctcga agcccgtttc
     4621 cggcgtggcg gcccgatccc cgacaccgcg cagggcgcgg cgctcgacca tctggcgcgc
     4681 ctcgccgacg cccttgtcca ccgacgcgcg acccgcgggc tcttcctgtg gggcggtgtc
     4741 gggcgaggca agacgcgcct gatggcctgc ctcgacgcgg tactgccgcc tgcagcaatc
     4801 cgccgttacc ccctcaacgc cctgcttggc gcctatcacg ccacctgtgt ctgccagccg
     4861 gactgggcgg cacggctcga caccctgttc ggcagcgccc gcgtgctgct gcttgacgaa
     4921 ttccatgcct acgacccggc cgacgccctg gtgctggagc gcctgctggc gggcgtcgcc
     4981 gagcggggca tggtctgcgt cgtcaccgcc aatcacccgc cggaccgcct gtggccggac
     5041 acaccggaac accgcgcgcg gcggcatcac tttgcaccgg tggcgcagcg gctggccgcc
     5101 cagtgcctgc tgctcgaggt ggacggcgga cgggattatc gggaattgct cgccgcccat
     5161 ggcccgctgc gctggtggtc cgcgacggat gccaccgatc gccaggccgc ccgacgctgg
     5221 ctgaccgcca ccgccggatg cgcccgggat ttcgacgagc tcttttgcca gccccggccc
     5281 cagcgagact acgtccgcct ggccgagacc gccggcggtc tgctgatcga cggctttccc
     5341 cgcctcaccc cgcgggatgg cgacgccctg cgccgcctcg tctggtgcct ggatgccgcc
     5401 tgggacgcag gtctggccct gggcgtcctg gccgaggccg caccagaaac ctgggtcgcc
     5461 ggcatcggtg ccgaactgga cggtctgctc ggcaaggatc tggcccgcgc ccgcagccgg
     5521 ctgcgggccc tgtgccgcgt cgacggcgcg ggcgtttcag ccgtagatct tgcacatgag
     5581 gggcgacgcg acccgcggtg agaaataccc cagggcgtag aagaaggcac acagcacggc
     5641 cacgccgatc accatacggc gatgcttccg ccacgtttca cgcaggctgt cgcggctcat
     5701 ttcaggtggc gcatcggctg taccctgggc agcctgcagc cggggcgcat gaaacggggt
     5761 gttccgtcgc cccatgccga gccgactgcg tggacggacg tcggcgcatc ctagaccttc
     5821 aggaagttcg cccggtaata acgcagttcg tcgatggatt cctggatgtc cgccagcgcc
     5881 tggtgggcgc cctgcttggt gaggccggcg tagacgtccg gccgccagcg cttgcacagt
     5941 tccttgaggg tggacacatc caggttgcgg taatgaaaga agtcctccag cttcggcatg
     6001 tagcggacca tgaagcggcg gtcctggccg atggagttgc cgcacatggg cgaggtccgg
     6061 gccggcacgt gggcctgcag gaaatccagg aacagggatt cggcggccgc ctcgtccacg
     6121 gtggaggcct tgacccggtc gatcaggccc gagcggccgt gggtgttctt gttccagtcg
     6181 tccatggcct ccagcaccgc atcgggctga tgcaccgcca ccaccggacc ttccgccacc
     6241 aggttgagct ggctgtcggt gacgatcatg gcgatctcga tgatgcggtc ggtgtcggga
     6301 ttcagaccgg tcatttccat gtccagccag atgaggtggt tcgggtcctg tgccataatc
     6361 ggctcgctcg tcggtaaagg cgcgattctg gcacatcgtc cggcgcctcg tcgcgcagtc
     6421 cgtgccgaac ttgcggctcg ccgccgcccc gctcactccc ctgccctgac ggcaggaacc
     6481 gtcctgcgcc ggtccgatcc aaccctcccc tgcctgcccg cccctcatcg atgtccacca
     6541 gcagtttcac caccctgttc ctcgtggccg tgcttgccgg cctggccgta cgcatcccgc
     6601 tggctctacg ccacgcccgc cacgtgcgcg cccaccgcaa cgccgtgccg cccgccttcg
     6661 ccacggccat cggcctcgat gcccaccgca aggcagcgga ctacaccgtg gcacgggtgc
     6721 acctggggct cgtggacctg ctggtgtcca ccgtctggct gctgatcctc accctgggcg
     6781 gcgggctgca ggccttgttc gacctcagcg ccggtgtcct cgacgccggc agcctgggtc
     6841 agggcgtcgc cttcctgacc ctggtcgcag tggcctcctg gctggtggat ctgcccctcg
     6901 gtctctatcg cagcttcgtg ctggaggccc gcttcggctt caaccgcctg acaccccgcc
     6961 tgttcgtcgc cgacaccctg aaaggcgccc tgctcgccgc cctgatcggc ctaccccttc
     7021 tggccggcgc cctgtggctg atgggcgcca tgggcgagca ctggtggctg tgggtgtggg
     7081 cggcgtggct cggcttcaac ctgctggcga tgctcatcta ccccagcttc atcgcgcccc
     7141 tcttcaaccg cttccagccc ctcgaagacg gcgacctcaa aacccgcatc gaggccctga
     7201 tgacgcgctg cggcttccgc ctctcgggcc tcttcgtgat ggacggctcc aagcgttcgg
     7261 cccacggcaa cgcctacttc accggcttcg gcggttccag gcgcatcgtc ttcttcgaca
     7321 ccctgctgga aaagctcgac ccggccgaag tggaggccgt cctcgcccac gagctcggcc
     7381 actatcacca ccaccatctc tggaaacgcc tcgccgtgat cggcaccggc agcctggcct
     7441 tcttcgcgct gctgggcgcc ctggcgcagc aaggctggtt cttcaccggg ctgggcatga
     7501 ccgcgaccgg tgcggcgccc acgctggtcc tgttctcgct agccctgccg gtgttcacct
     7561 ttcccctcac gccgctgatg agccactggt cgcgccgcca tgaattcgag gccgatgcct
     7621 acgccgcccg ccaggcgccg gccgccagcc tgatctcggc gctggtcaag ctgtaccggg
     7681 acaatgcctc gacgctcacg ccggacccgc tctactcccg cgtgtatgac tcccacccac
     7741 cggcggcggt ccgcatcgcg cgcctcgagg cggctgtccg gtgaaggccc gcggcaccct
     7801 ggtggcggcc ttcgggcggc agtacgaagt ccgcaccgac ggcggcgaga tcctgttgtg
     7861 cgtaccccgc ggcaaaaaga gccgcttcgc ctgcggtgac cgggtccagg tggcccgcag
     7921 cgcgcccggc cagggcgtca tcgagcaact cgaagaacgc ggcagcctgc tctaccgctc
     7981 cgacgcctgg cgccagaagc tcatcgccgc caacgccacc caggtggttg tggtggtggc
     8041 caccgagccc agcttcagcg acgatttcgt gacccgctgc atctgcgccg ccgaaagcca
     8101 gcagctcaag agcctgatcg tcctcaacaa ggccgacctg cccgccgggc tcgacgccgc
     8161 ccgcaccctg ctggccccct tcgccgcgct ggactacccg atcctggagc tctccgccct
     8221 acacgacgtg gacgccctgc gggcgcgcct caaggacgag accagcctgc tcgtgggtca
     8281 gtccggcatg ggcaaatcca ccctcactaa cgccctgatt cccgaggccc gtgccgaaac
     8341 ccgcgaaatc tcggaagtcc tcgacaccgg caagcacacc acgaccttcg cccgcctcta
     8401 cgacctgccc ggcggcggcg gcctgatcga cagccccggc atgcagacct tcggccttgc
     8461 ccacctggac aatgccgccc tcgaagccag cttccgggag tttcgcccct gggacggcca
     8521 atgccgcttc cgcgactgcc gccacgacag tgaaccgggc tgcgccctgc gtgccgcggt
     8581 ggccgatggc cgtatcgcgc cacgccgcct cacccagttc ctggaactga gggccgagtg
     8641 tgagcaggcc cgcaagaccg cccagggctg gtagtcgggc cccgcatcgg ccggttgcga
     8701 acgcgatcgc cgcccccgtc agcggaccgt tatcgaccct ccgcaacgac ggcgcgccat
     8761 gccctgttcg ccattccgta gcctgtcaga ccagtcaccc aaaaagaaaa ggcggggact
     8821 caccccgcct tcagcgcatc gtcacccgcc gcgtcctcag ccctgcagat ccttcaggat
     8881 ggcctggatc tcaccacgga tcgacgcgag ctgatcgcgc aggacggcgg actcctccac
     8941 gtcctcccgc ttacgtgagg ccatctcgtc gagacgattg cgcgcgccgg agatggtgaa
     9001 gccgtcccgg tacagcagct cacggatgcg gcggatcagc agcacctcgt ggtgctggta
     9061 gtaacggcgg ttgccgcccc gcttgaccgg ccgcagctgg gtgaattcct gttcccagta
     9121 gcgcagcacg tggggtttga ccgcgcacag ctcgctcact tcaccgatcg tgaaatagcg
     9181 cttggccggg atcggcggca gctcgacgaa ctccgttacc tcactttgcg acatcactca
     9241 gatgctccac gttggctttg agcttctggc tggcgtggaa ggtcaccaca cgccgggccg
     9301 tgatgggaat ctcttcgccg gtcttgggat tacggccggg acgctggggc ttttcgcgta
     9361 gctggaagtt gccgaaaccg gacaatttca cgcagtcgcc ccgctccagg gccaggcgga
     9421 tctcctcgaa gaagcactcc accatgtcct tggcttcgcg cttgttgaga cccacctgct
     9481 cgaacagcat gtcggcgagt tccgccttgg tcagagtcac gcccatggtg atcaccctcg
     9541 cagtttcgca ccgagggctt cctcggcgtg gcgggtcagg gcggtcattg cggcttccac
     9601 ttcagcgtcc tccaaagtcc tctgagtttc ttgcataact acgcgaaaag caaggctttt
     9661 ccggttcggt tcgatacctt tgccctggta ttgatcgaac aactcgatac gttggacgat
     9721 ggccggcgcg acactcgaaa gtcctgcaat gaggctcgcc accggcgtcg cctgatccac
     9781 caccagggcc agatcgcggg tcaccgccgg aaagcgggag acttcgcgat aggcgggcgt
     9841 catggtctcc agcaacgacg tgagcgacac ctcgaagacc accggcgcag tgcccagctc
     9901 gtagcgctgc acccacaccg gatggagttc gccgatcacg ccgatctcct ggccctccag
     9961 caacacccgg gcagcgcggc cgggatgcag ggccggatgg accaccggtt cgaaggtcgc
    10021 gacgcgggga tggagcagcg cctccaggtc cgccttcacg tcgaagaagt ccacagtgcg
    10081 gacggcctcg ccccattgct cggggcgcag cggccccgcc gccagggccg ctacgcgcac
    10141 cggctgatga aagccttcca ccggcgcacc cgcggcgtcg cgacggaata tgcgaccgag
    10201 ctcgaagacg cgcacccgat tgttctgacg cttgcggttg gtgaccacgt tggccaccag
    10261 accgccgatc aggctggagc gcatcacgct catctggctg gcgatggggt tggcgaggcg
    10321 caccgggtcg gcattgccac agaaatcccg ctcccaggcc tcttccacaa aggaaaaatt
    10381 gaccacttcc tggtattccc gcgcggccac gaggcggcgg acgcccatcg gatcgcggcg
    10441 cgcctcgggg cgcggcagca tggcgatggg gcccttgggc acgacggtgg gaatgttgtc
    10501 gtaaccgtgc agacgggcga tttcctcgat gaggtcttcc tcgatctcca ggtcgaagcg
    10561 gtaggacggc ggcgtgacga cgaagtcgtc gccatcgcga ttcacggcca gattgaggcg
    10621 gccaaacaga tctgccacct cgtccgccga gacgtcgatg cccagcaccc ggcgcacgcg
    10681 ggccgcgcgc aggcggatcg gcgcccgctg cgggagtcgg tcggctgcca gggcttccac
    10741 caccgggccg gcttcgccgc cgcacaggtc gaggatcagg cgcgtggcac gctccagggc
    10801 ggccggcgcc agggcgaagt ccacgccccg ctcgaaacgg taggaggcat ctgacgagaa
    10861 gccataggca cgcgcccggc cggcgatggc gtcgggcgca aagaaggcac tttccaggaa
    10921 caggtcggtg gtttccaggg tgatgccgct ctcctcaccg cccatgatgc cggccagggc
    10981 cagggcacga gactcatcag cgatcagcag ggtgtcggct gccggcgtga cggtctggcc
    11041 gttgaggagc agcacctgct cgcccgcctt gggcatgcgt acgtggatgg cgccgtcgag
    11101 cctggcgttg tcgaaggcat gcagcggctg acccagctcc agcatgacgt agttggtcac
    11161 atccaccagc acgctgatgg aacggatgcc gctgcgggcg aggcgctgct tcatccaatc
    11221 cggcgttggc gcctgggcgt tcacgccgcg gacgacgcgg ccgcagtagc gcgggcaggc
    11281 ctcgggggcg tcgagcacga ccttgcgggt gtccgcgatg gtgggcgcca cggaggacgt
    11341 gtccacgaga ctgaggggac gatcggtcag cgccgccact tcgcgggcga tgccggtcag
    11401 gctcaggcag tcggcccggt tcggtgtgag cttgatggtg tagacggtgt cgtcaagggc
    11461 cagatagtcg cgcaggtcgg tgcccaccgg cgcatcctcc ggcagcacca gcaggccgct
    11521 gctgtcgtcg gcgatgccca gctccttggc cgagcacagc atgccgaagg attcgacgcc
    11581 gcgcagcttg gccgccttga tggcgaagcc aggcagttcg gcgcccacca gggcacaggg
    11641 aatcgtgagg ccggccgccg cgttaggggc gccgcagacg atctgcagcg gttcgccctg
    11701 accggcatcc accttgcaga cgcgcagctt gtcggcgttg ggatgcttgt cggcttcgag
    11761 gatgcgggcc accaccacct tggagaaggc cgcggccaca ccgccctgct cttccacttc
    11821 aagccccgcc atggtgagca gatggccgag ggcgtcggcg tcgagggccg ggctgacgaa
    11881 actgcgcagc cagagttctg agaattgcat ggcttacctg aattgaccga ggaaacgaag
    11941 atcgccttcg aagaacaggc gcagatcatt gacgccgtag cgcagcatgg tgagccggtc
    12001 gggccccatg ccgaaggcaa agccggtgta gcgctccgga tcgatgccgc cgaatcgcag
    12061 cacattggga tggaccatgc cgcagccggc gatttccagc cagcggccct tgagcgcgcc
    12121 gctcatgaag gccacgtcga tctcggcgga cggctcggtg aaggggaaga aggacggccg
    12181 gaagcgcacc tgcaggtcgt cgctctcgaa gaagcggcgc aggaagtcgg cgattacgcc
    12241 cttcaggtcg gcaaaactca ccgattcgcc gacccacagg ccttccacct gatggaacat
    12301 gggcgaatgg gtggcgtcag aatccacccg atacacgcgg ccgggcgcga tgacgcgcaa
    12361 ttcgggcatg ccgtcgcggt ccttgtaggc atcgacatgg gcctgcatgg cacggatctg
    12421 caccgggctg gtgtgggtgc gcaggagcac gccttccccg ttttccaggt agaaggtgtc
    12481 gtgcatggaa cgggccgggt ggttggccgg cgtgttcagc gcagtgaagt tgtagaaatc
    12541 ggtctcgatc tccgggccgt cggccacttc gaagccaatg gagcggaaca gctgctcgac
    12601 acgctcaagg gtgcgcacca ccgggtgcag accgccaaga ccggcgcccc ggccaggcaa
    12661 ggtcacgtcc agtgcttcgg ccgccagctg agcttccagc gccgcctggc gcagggcttc
    12721 acggcgggcc tccagggccg cttcgatggc ggacttggcc ttgttgatct cagcgccggc
    12781 ggccttgcgc gcctccgcgt cgagcttgcc gaggcccttg aggagttcgg tgatggagcc
    12841 gcccttgccc agaaaacgcg ccttggcggt ttccagcgcg gcactgtcgg cggcggcggc
    12901 gaaatcggcg cctgcctgcg cgacgagttg atcgagttga tccatttttc agtcgaatgc
    12961 cgggttcagg ataaaaaaaa ggaggccagg cctccttttt ttatgctgcg ttggatcacg
    13021 cagcgagttt ggccttggct tgctcggcga gcgccgcaaa cgcgggcttg tcgaatacgg
    13081 ccagatcggc gagcaccttg cggtccactt cgatcgcggc cttcttcagg ccattcatga
    13141 agcggctgta ggtcaggccg agctcgcggg acgcggcgtt gatacgcgcg atccacaggg
    13201 cgcggaactg acgcttgcgc tgacgacggt cacgataagc gtactgaccg gccttcatca
    13261 ccgcctgttt ggcgatgcga tagacgttct tgcgacggcc gcgatagccc ttggcttgat
    13321 cgaggacctt cttgtgacgg gcacgggcgg ttacaccgcg tttgactcgg ggcatggcgt
    13381 tctcctaatt caggcgtaag gcatcattgc gcggatggac ttttcgtccg aggcatgcac
    13441 gcaggtcatg ccgcgcaggt ggcgcttgct cttggtggtc ttcttggtca ggatgtggcg
    13501 cttgaacgcc tgggaacgct tgatgctgcc gctcgagcgg accttgaacc gcttggcggc
    13561 tccgctcttg gtcttcatct tgggcatgaa ggactccttt tatgtcatgg ccccgggtgg
    13621 catccgactt ccggacacgc ttcaacaacc cgtcaccact tgtgcccggc tggctttttt
    13681 cgccggccgg atttccggcc cctcgcagga ccgggaattc aatacgcgct cagtgcttgc
    13741 gcttgggcgc gatgatcatg atcatctggc gcccttccat cttgggcatc tgctccacct
    13801 gggcgatttc ctccaggtcg gccttgatgc gctccagctg acgcatgccg aattcctggt
    13861 gcgccatttc gcgcccgcgg aaccgcagcg tcaccttggc cttgtcgcct tcgttcagga
    13921 acttgatgag gttgcgcagc ttgatctggt agtcgttttc gtccgtaccg ggacgcaatt
    13981 tgacttcctt gacctggatc tgcttctgct tctgcttggc ctcgtgcgcg cgcttggctt
    14041 cttcgtactt gtacttgccg aagtccatca gacggcatac cggcggcttg gccatcggtg
    14101 cgatctcgac gagatccaca ccggcttcat ccgcggcttc cagtgccgca cgcagggaca
    14161 cgattccgat ctgttcgccg tccgggcccg tcagacgcac ttccggcgca gtgatttcgc
    14221 cgttgatgcg cagcttcttt tcctgagcga tggttcaagc ctccttgaaa aacaaaaaat
    14281 cacgccgtgc cggctcgcgc gagaagctca cgctgccaac gctcgacaac ctgttcgagg
    14341 gacatttgac cgaggtcttg attgccacgg gcgcgcactg cgacgacatc tgctgccttt
    14401 tctttatcgc cgatgaccag caggtaaggc agctttcgta cgctatgttc gcgtatttta
    14461 taagtaattt tctcgttccg caaatccgct tcaacgcgga agccgcgtga acgcaagcga
    14521 ttcaccactt ccgtcacgta atcggcctgg gcttcggaga tattcatcac cacagcctgc
    14581 accggagaga gccacagcgg caaggcgccg gcatggtttt cgatgaggat accgatgaaa
    14641 cgctccaggg aacccagaat ggcccggtgc agcatcacgg gacgatatcg ggcgttgtcc
    14701 tcggccacat attcggcgcc aaggcgctct ggcagcacga aatcgagctg catcgtgccg
    14761 cactgccagg accggccgag ggcgtccttg atgtgatatt cgatcttcgg accgtagaac
    14821 gcgccctcac ccggcagttc ctcccaggaa tgcccggacg cagccaatgc ctgacgcaga
    14881 ccggtttccg ccttgtccca gatggcgtcg gtcccggccc gcttctcggg gcgcagggag
    14941 agcttgaccg ccacatcggt gaagccgaaa tccctgtaca cgcgggtcag cagttcgttg
    15001 aaggccgtca cctcggacac gatctgctct tcggtacaga agatgtgggc atcgtcctgg
    15061 acgaagccgc gcacccgcat caggccatgc aacgcgccgg acggttcgtt gcggtggcag
    15121 gagccgaatt ccgcgaggcg cagcggcaag tcccggtagg aatgcaggcc gtggttgtag
    15181 atctgcacgt gaccggggca gttcatcggc ttgatcgcgt agtcgcgctt ctccgactcg
    15241 gtcgtgaaca tgttgtcgcg atagttgtcc cagtgaccgg atttctccca cagggcgcga
    15301 tccatcacca gcggcgtctt cacctcctgg tagcccgcct cattgaggac gctgcgcatg
    15361 tactgctcga cctgttgcca cagggtccag ccgtggggat gccagaacac catgcccggc
    15421 gcctcatcct gaagatggaa gagatcgagc tggcggccaa gccgccggtg gtcacgcttt
    15481 tcggcctcct ccagcatgtg caggtaggcg tcctgatctt ccttctttgc ccacgcggtg
    15541 ccgtagatgc gctgcagcat ctcgttgcga gagtcgcctc gccagtaggc gccggccact
    15601 ttcatcagct tgaagacctt gagcttgccc gtcgaaggca cgtgggggcc gcggcacagg
    15661 tcggtgaagt cgccttcctt gtacagggaa acatcctcat tggcggggat ggaagcgatg
    15721 atctcggcct tgtagtgctc gccgatggac ttgaagtagg ccaccgcctc gtcccgcggc
    15781 aacacgctgc gttcgacggg aatgtccttc tttgccagtt ccagcatgcg cgcctcgatc
    15841 ttcgccagat cctcaggcgt gaacgcgcgg gtataggaga agtcgtaata gaagccgttc
    15901 tcgatcaccg gtccgatggt cacctgcgcg tcgggaaaca gcgccttcac ggcataggcc
    15961 agcaggtggg cggtggagtg acggatgagc tccagcccgt ctgcgtcctt gtcggtgacg
    16021 atggcgagct gcacgtcccg gtcgatcagg aaggaggtat ccacgagacg cccgtcgacc
    16081 ttgccggcca gggccgcgcg ggcaaggccg gcgccgatgg aggcggcgac ctgggcgatc
    16141 gtcacgggct gatcgaagct gcgtacagaa ccgtcgggca gggtgatgtt gggcatgatg
    16201 actggctcag ggcaaaaaaa aagtgcggac gagccgcact tttttcatga ctggaaaacg
    16261 ccttgcagcg gactcgaatg catcaatcgg agcggctgca agttcggaac atccgttttc
    16321 cttctcaggt gtttggtagg cgcgattgga ctcgaaccaa cgacccccac catgtcaagg
    16381 tggtgctcta accaactgag ctacgcgcct aagaagctgt gcattataaa ggctgagaga
    16441 aggaagtcaa gattattttg gactatcgga ttatgaactt ccatcgcccc aggcttccag
    16501 aaaacgctgc cagtgacgtg cgggcaggct cgacaggctc ttgcgcacga aggcgatctc
    16561 ttcttcatag cgctcggcgc cgaggatccc gcgcatgcgc tggaagcgca gcagcacaag
    16621 ataggtattc accacgtccg tttcgcagta atcccggatc tcggcgatac gcccatcccg
    16681 gtagccctgc cagacggctg agccatccat gcccagctta ccgggaaaac ccatcagctt
    16741 cgccagatca tccagcggag cgctggcccg cggctgatag agggccagca ggtccatcag
    16801 atccaggtgg cgggtgtggt agcgcccgat gtagttgttc cacttgaagt cgcgggaatc
    16861 gaaatagtcg ccatccccga gatcccagta cctggatgcg gcaacaccat gcatcatgcc
    16921 ccggtagtgc agcaccggca gatcgaagcc gccgccattc caggatacaa gctggggggt
    16981 gaatttctcg acgccgtcga agaagcgctg gatgatctgc ccttccgtcg cgtccggttc
    17041 cgacagggaa aagacgcgga actggctgtc gtcccgcagc acgcacgaga ttgtcacgac
    17101 acgctgcagg tgatggggca gaaaatcgtt gccggtggca gcacggcgct gctgaaaggc
    17161 gaactcggcc acctcgtcgt cgcccagttc tgccggcagg ccgttcagcg cgcggatgcc
    17221 ggccacatcc gggatcgtct cgatgtcgaa gaccaggatc ggaaccatga acgctcaggc
    17281 cggaaacacg ccggtggaga ggtagcggtc accccggtca cagacgatgg acacgatcac
    17341 cgcgtcctcc agctgggcgg cgaggcgcag ggccacgtgc aacgcaccgc ctgacgagat
    17401 gccggcgaag atgccttcct cccgcgccag gcggcgggtc atctcctcgg cgtctgcctg
    17461 ggccacgtac tcaagctgat cgacccttgc cttgtcgtag atgcgcggaa gataggcctc
    17521 gggccacttg cggatgccgg gaatctgggc cccgtcggca ggctggcagc caatgatgcg
    17581 gatcgccggg ttgcgcgcct tgaggtaggt ggatacgccc atgatggtgc cggtggtgcc
    17641 catgctgctc acaaaatggg tgaccgtgcc gccggtctga tcccagatct cgggcccggt
    17701 accttcgatg tgggccagcg gattgtccgg gttggcaaac tggtcgagga tcacgccgcg
    17761 accttcgtcg cgcatgcgct cggccacgtc ccgcgccagt tccatgccgc cctccttggg
    17821 cgtgagcacc agctcggcgc cgaaggcccg catggtctgg cgccgctcga tgctctggtt
    17881 ctcgggcatc accaggatca tgccgtagcc gcgcatggct gccgccatgg ccagggcgat
    17941 gcccgtgttg ccgctggtgg cttcgatgag cgtctgtccg ggccggatgt cgccccgcgc
    18001 ttcggcgtga aggatcatgg acagcgcggg ccgatccttg accgagccgg caggattatt
    18061 gccttccagc ttggccagga tcgtattgtt gcgcccagcg gtgatgcgtt tgaggcgaac
    18121 caggggcgtc tggcccacgt agtcctcgag tgtcttgaat tccatgtgac agtccggtgg
    18181 atgcggggat tggggcggat cataccggat ggcgcccccg atcatcgcaa gcatgcgccg
    18241 cgccagccgg acaaacgcgc ccactgcgcg ggattcgtcc gtccggcctg accccagcga
    18301 caaaaattgt cgggttcatg ccacccactt ccgatccggc cgcagacatc cttgtgcaag
    18361 gcgcgcctca gggcgtcaga tcgaattcct cagtgcgccg cggcggatag gtttcccaac
    18421 cgccgcaggc cgggcagcgc cagtagaact gccgtgcctt gaaaccgcag gcatcgcagc
    18481 gataacgcgc cacccgccgg gtatggccat gaaccagacc cttgaccagt tcgatatcgg
    18541 tgcgctgttc gaccggtgcg gtgagcaagg ccgcttcgag caatttgtcg aggcccagca
    18601 gggtgggatt gcggcgcaac tcctcgcgga ccaggtgata cgccgcctgg gccccctcct
    18661 tctggagttc ccactggaag accgcatcca gcagatccag cgaggcgtag ctgtccaggt
    18721 agctgcgcag gagctgcatg ccctgctcca gtcggccgag gcgccgatag gcgtccatga
    18781 tgcgttcggc caccagtgcc agatagaccg gattctgggt ctcgacacgc ttccaggcct
    18841 ccagcgctgc ttcgtcgtca ccctgggcgg catgaagatc acccagcagg atgctcgcgc
    18901 gcaccgactt gcgattggtc tcaagggccc gctccagatt cgaacgcgcc tcatcaaagc
    18961 gcgaatcggc gatggccgct gccgccagtt cacagtggaa gttggcgacc tctttttccc
    19021 acagggcgct ctcatggccc ggcagcgcct gggcgatgtc gatggccttg cgccagtcct
    19081 tttcctgctg gtagatgtcc aggagattct tgagggcgaa ttcgtcgagc cgggagcccc
    19141 gcagctgaac gaagaccgcc tcggcccggt cgagcagacc ggccttcagg tagtcgagcc
    19201 ccaactccga cagggcctgc aggcgttgat cctgggacag gtcgtcccgg tcgatgaggt
    19261 tctggtgcat gcggatcgcc cggtcggtct cgccgcgccg gcggaacagg ctacccaggg
    19321 cgaaatgcag ctcgacggtc tgattgtcga tcttgaccgc ctcgatgaag gcatcgatgg
    19381 ccttgtcggg ctgctcgttg aggaggaaat tgagaccggt caggtaggtc cgcggcaagg
    19441 cgcgggattc cttgaccaga tgcttgatgt cgatccgcgc ggccagccag ccaagtgcga
    19501 agaacagcgg aaacgccagc agccaccaga gttcgaattc catgggggat cagtaggtct
    19561 cgggcagatc ggggccgtgg gccacgggcg ggcggacagt cacggggggc ggcgcatcgg
    19621 cctgacgctc acgcagttgc ccaagctccc gatgctggcg gtacagggtc gcgaccgcgg
    19681 cggtgactcc gatcagcacc ccgatcacga caaagagcag gatcaccagc accagcggca
    19741 cctgccactg ggcaccgaag aaaaaccgca gggtgactac atcgtcgttc ttgacggcaa
    19801 agccgaagag gacgaggaac aggaacaggc ggacgatcca ggtgagcagg cgcatgacga
    19861 aattatcccc gacgggacca aaaaaaagaa ggcggcaatc gccgccttcc agtatgggcc
    19921 gaaccagacc gcggcacgcc gcggcgatca gacaatcatg tccacccgct cacgcagctc
    19981 cttgccggcc ttgaaatggg gcacgtactt ttcaggtacc tgcaccttct cccccgactt
    20041 cggattgcgt ccaacgcggg gcggccgata gttcaaggca aagctgccaa agccgcggat
    20101 ctcgatgcga tcaccccgcg agagggcgtc ggtcatcgca tcgaggatca tcttgacagc
    20161 ggtttcggcg tccttcgcca ccagctgggg aaaacgctcc gccagccggg cgatgagctc
    20221 cgacttggtc atgccggtgc cgcttacttc tgttcgttga gcttggcctt gagcagcgca
    20281 cccaggttgg tggtgccgct ggcagccgag ccgctgtcgg aagcgagctt gttcatggct
    20341 tcggcctgtt cgacctgatc cttcgcacgg acggacaggt tgatgccgcg ggtcttgcga
    20401 tcgacgttga cgatcagggc ctcgacggcg tcacccacct tgagcagctg ggacagatcg
    20461 tccacgcggt cacgggagaa ctcggaagca cgcaggtagc cttccacgtc atcgttcagg
    20521 gccaccaccg caccgcgggc atccacggac ttgacggtac cggtcacgag ggtgtttttg
    20581 tcgtgggtgg cgatgaagtt ggtgaagggg tcaccttcga gctgcttgat gccgagggag
    20641 atgcgctcct tctcgacgtc gatgttcagc accacggctt ccacttcgtc gcccttcttg
    20701 tactgacgca cggcttcttc gcccgccagg gaccaggaca ggtcggacag gtgcaccaga
    20761 ccgtcgatgc cgccttccag gccgatgaac acgccgaagt cggtcaggga cttgatctga
    20821 ccacggacct tgtcgccctt cttgtggttg atggcgaaat cgtcccacgg gttggacatg
    20881 cactgcttca tgcccaggga gatgcggcgg cggtcttcgt cgatctcgag gatcatcact
    20941 tcgacctcgt cgcccagctg gacaaccttg gtcgggtgga tgttcttgtt ggtccagtcc
    21001 atttcggaga cgtgcaccag gccttcgatg ccctgctcga cttccacgaa cgcaccgtaa
    21061 tcggtgatgt tggtgacctt gccgaacagg cgggtgccct gcgggtaacg gcgggagatg
    21121 ccgacccacg gatcttcgcc gagctgcttg agacccaggg agacacggtt cttctcctgg
    21181 tcgaacttga gcaccttggc ttcgagttcg tcacccacgg acagcacttc ggaggggtga
    21241 cggacgcggc gccaggccag gtcggtgatg tgcagcaggc cgtcgatgcc gcccagatcc
    21301 acgaacgcgc cgtagtcggt gatgttcttg accacgccct tgacgacggt gccttccttg
    21361 agggtttcga gcagcttctg acgctcttcg cccatggatg cttccagcac ggcacggcgg
    21421 gacacgacca cgttgttgcg cttgcggtcg agcttgatga ccttgaattc gaattccttg
    21481 ccctcgaacg gggtggtgtc cttgaccgga cgcatgtcca ccagggagcc gggcaggaag
    21541 gcgcggatgc cattgaccat caccgtcagg ccgcccttga ccttgccggt gatcacaccc
    21601 ttgaccagaa tgccttcgtt gagggccttg tcgaggtcgt tccaggcggc gatgcgcttg
    21661 gccttgtcgc gggacaggcg ggtttcgccg taaccgtctt cgagttgctc gatggcaacc
    21721 tggacgtaat cgcccacgtt ggcggtcagt tcgccgcggt cgttgcggaa ttcgtcgagg
    21781 gggatgtagc tctcggactt caggccggcg ttgacgacga cgaagttctg gtcgatccgc
    21841 acgacttcgg cggtgatcac ttcaccggca cgcatctcct gacgattgag gctttcctcg
    21901 aacagggcgg caaaactttc ctcgatggaa acaggggtgg caatggacat attgaaaaaa
    21961 taccctacaa ccgtcgcgag acggctcctg gttgaaaaac ctccccggac cgggcagacc
    22021 ggggagaacg cgtcaaaaaa agggatcagc ccttcaggat cgcggcccgc tccagcacaa
    22081 acgccacagc ctgttccaca gacatatcgg acgtgtccag cagcaccgca tcctcttcct
    22141 gcttcaaggg tgcggccgcg cgctgggcat cccgggcgtc gcgttcacgc aaatccttga
    22201 gaagatcggc catgttagca ggcattcctt tatccatcaa ctgcttatag cgcctttccg
    22261 cacgcgcctc gacggaagcg gtgaggaaga tcttcacggg cgcatcggga aagaccaccg
    22321 agcccatgtc gcgcccttcg cccaccagac cgggggcacg gcgataggcg cgctgacgcc
    22381 agaacagggc ctcacgcacg gtcggcaggg ccgccacgcg ggacgccccc aacgagcagg
    22441 cttcgctgcg gatcgcctcg gtgacgtcgt cgccctccag catcacacga tcaccctcga
    22501 agcgggccgg cagatcggtg gcgatggcgg ccagcgtcgg ctcgtcgtcg agggccacac
    22561 cgcggcggat cgccgtcagg gcgacaatgc ggtacaggga tccgctgtcc aggtgcgcaa
    22621 agcccagagc ctgggcaacc cgcgcagcca cggtgccctt gccggatgcc gacggtccgt
    22681 cgatggccac caccggcacc ggcgcggtga tgccggcgaa ggtctcgaaa tagccgggga
    22741 aggtcttgtt gacgcacttc ggatcgttga tgcgcaggct ggtgccgcac agggtcgcca
    22801 aagagaagca catggcaacg cggtgatctt cgtaggtatc gatcaccgca ggctgcaggc
    22861 gcgcgggcgg cgtgatccgc agactctcgg ggaactcctc gacggtcgcc ccgagcttgc
    22921 gcagttcggt ggccatggcg gcgatgcggt cggtctcctt gacgcgccag cttccgatgt
    22981 tgcgcagatg gctcgggccg tcggcgacga gggccgccac ggccagggtc atggcggcat
    23041 ccggaatgtg attgagatcc aggtcgatgc ccttgagtcg ccctgactcc ggcgcccgcg
    23101 cctcgatcca gtcctcgccc caggtaatct gcgcgcccat ttcggcgagc gcatctgcaa
    23161 agcgcacgtc accctgaatg ctgccgcgtc cggccccttt cacccgcagc gggccgcccc
    23221 ccagagcgcc tgcggcaagg aaatagctgg ccgtggaggc atccccttcc accgcgatcc
    23281 gtcccggcga cacgtagcgc tggccggcca gcacgacgaa gcggttccag ccgtcccgct
    23341 ccacctgcac cccgaagcgc gccatcagat tgagggtgat ttccacgtag ggccggctga
    23401 tcaactcgcc ttccacgacg atcacgaggg gcgtgtcgcg ggccaccagc ggcgcagcct
    23461 ggagcaggcc ggtgaggaac tggctggaga cgttgccgcg cacgctcacc gtctccgcgg
    23521 cgaggggcgc cggacgcagc gccaggggcg gaaaccccgg ctggccgagc caatcgacgc
    23581 gggcaccgag ctgagccagg ccgtccacca gatcgccgat gggccgttcg tgcatgcgcg
    23641 gcacgccgga caggcgcacc gccgcatccg catccccgca gcccgccagg ctggccacga
    23701 cggccggcac cagggtgcgg atgctgagcc cggagttgcc cacgaagagg tcggcccgcg
    23761 cgttcgggaa acgcccgccg caacccgtca ccaccagcgt gtcaccctcc tgttggaggc
    23821 caacacccag cgccacgagg gcgtcgcgca tgacgcgggt gtcgtccgaa tccagcagcg
    23881 cctcgacacg cgtcgtgcct tccgccagcg ccgccagcag cagcacgcgg ttggagatgc
    23941 tcttggagcc gggcaggcgc acctcgccga cggccctgag catcggcggc aaatcgatga
    24001 attccatgaa agatccttga attactcgcc cgaaggcgtc gcgggaaaag ccttgtcggc
    24061 ccattcgttg cgggcccggc tggcctcgga gaaaaccttt tccagggccg gaccatcgcc
    24121 cgaggcgagc agcgcccgca gccaggcgag ttcgcccagg taagcgtcca gctcggcgag
    24181 cagggaatga cgattggcga gacagatgtc gcgccacatt tccggatgac tgccggccac
    24241 ccgggtgaaa tcgcggaacc cgctcgcggc gtagctgaag agctcctccg cgttggcacg
    24301 cccggccagg tcatgcacca gcccgaaggc cagcagatgg ggcagatggc tcaccgcggc
    24361 gaacacccgg tcgtgctcct gaggcgtcat ctcgaagagg tgcgcaccgc aggctgccca
    24421 cagttcgcgc acccgcgcga cggccacggc atcgttctct ggcaggggcg tgaccaccac
    24481 cttgcgcttg cggtacaggt ccgcccgcgc cgccgacggg ccgctctttt cggctcccgc
    24541 gatgggatgg gccggcacca cgcggggcag gtgggcggcc aggtggcgat agatggcctc
    24601 gatgacatca cgcttggtgc tgccagcgtc ggtgaccagg gtgtccggcc ccagatgggg
    24661 cgccatggcg gcggccaccg catcggtctg ccccaccggc atggccagca ggacgatctc
    24721 ggcgccatcg agggcctcgg cccagtcggt ggagatctcg tcgatgacgc ccagctgcag
    24781 ggcctgttcc agggtttcgc ggcggcggcc gacgccgacg atgcgattca ccaggcgcgc
    24841 ctggcgcagg gccagggcca ccgatccgcc gatcaggccg acgccgcaga tgaccagctt
    24901 gtcgatcttg cgcaccgcgc cgtcctcagc ccagtgcctg gggcagcgct tcgaggaagc
    24961 gcgcgttctc ggcttccagg ccgatcgaga cgcgcagcca ttcgggcatg ccgtagccgg
    25021 cgatggggcg cacgatgacg ccctggcgca gcaggcgccg attgacaccg gcaccgtcgc
    25081 ctgccctgaa cgtgacgaaa ttgcctgccg acggaatcca ctccagcccc aggcgggcga
    25141 agccggcggt gagctgggcc atgccggcac ggttcacttc atagctgcgt gccaggaaat
    25201 cgctgtcgaa aagtgccgca gcggctgccg ccagggccac gctgcccacg ttgaagggct
    25261 ggcgcacgcg gttgagcagg tcgatgaccg ccggatggcc caggccgtag cccacccgca
    25321 ggccggccag cccgtaggcc ttggagaagg tgcggcacac cagcaggttg gggaagcggg
    25381 ccagccaggc aatggcgtcg tagcgctgtt cctcgccgag gtattcggtg taggcctcgt
    25441 cgaggaccac cagcacgtgg tcgggcacgc ggacgaggaa ggcttccagt tccgggccgg
    25501 gcacgaaggt gccggtggga ttgttggggt tggcgatgaa gagcacccgg gtgtccggcg
    25561 tgatcgccgc gagcatggcg tccagatcgt ggccgaagtg cctggccggc acctcgacgc
    25621 cccgcgcacc aacggcctgg gtggccagcg gatacacggc aaacgcgtgc tgggcgtaga
    25681 tggccgaggt ctgcggacct agaaaggccc gcgcggcaag ctccaggacg tcgttggagc
    25741 cgttgccgag gacgatctca tccatcgcca cgccgtagcg acggcacagg gcctgtttca
    25801 gctcgaagcc gttgccgtcc gggtagcgcg ccagatcgtc gagggctgcg gccatcgccg
    25861 cccgcgcctt gtcgccgaag ccgaggggat tctcgttgga cgccagcttg acgatggacg
    25921 actccgccag ccccatctca cgggccagct cggcaatggg cttgcccggc tgatagggtg
    25981 aaatcgcgcg gatgtgggcc ggcgacagat cgcacagacg catggaaggc tccgggggtt
    26041 cagtacacag cctggggata ggaccccagg atcttcagga aagaggcgtg ggcgctcagg
    26101 gcgtccaggg ccttgcggac ctccggatca ttgcgatgcc cggcgaagtc cacgtagaac
    26161 acgtattccc acagggccgc ccgcgccggg cgggattcga gccgggtcat ggacaccccg
    26221 gcgtccgcca ggggcgccag cagcgcgtgc atggcgcccg ggcggttggc tgcagacatg
    26281 atcagagagg tcttgtcggc gccgctggga ccggcgtcat gacggcccag caccaggaaa
    26341 cgggtggtgt tgttgggctc gtcctcgatg ctgcgggcga gggtcggcag cgcgtagcgc
    26401 tccgccgcgg cctcaccggc gatcgccgcc gcgccgggct cggaggccgc caggcgggcc
    26461 gcctcggcgt tgctggccac ggaaatgcgc ggcacgccgg gcagatggct gttgagccat
    26521 tcgtgacact gggccaggga ctgggcgtgg gaatacacct tgcggacgtc gcccagcgca
    26581 gtctcaccgc tcatcaggtg ctgatggatg cgcagcacga cctcgccgca gatctgcagg
    26641 ggcgaggaca gcagcaggtc gagcgtgcga ccgacggcgc cctcggtgga attctccacc
    26701 ggcgccacgc catactgggc gtgaccggac tccacttcac ggaacacgtc gtcgatggtg
    26761 gtctggggcg ccagccgcgc ggcgtggcca aagtgcttga ccgcggcgct ctcggaaaag
    26821 gtgcccagcg gcccgagaaa ggcaacgccc aacggatttt cgagggacag gcaggcggac
    26881 atcacctcgc ggaagaagaa ggtgacgctc tcgtccggca gcggaccggc gttgagggcc
    26941 tggatgcggc gcagcacctg ggcttcccgc tccggccggt agacctgccc ggcctcaccg
    27001 tggcgggcct tgatctcgcc cacgtgctgg gcacatctgg cgcgcgcgtt gagcagcgcc
    27061 agcatctggg cgtccagtgc atcgatctgg tcgcggaccg caccgagttc ctgcagcatc
    27121 gcctcgttca tgcctgctcc cgaaagtccg gcgctcacgc cttgcgccgt gcgaaatcct
    27181 gcatcagatc caccagcgcc tcgacaccgt caagggtcat ggcgttgtag atcgacgcgc
    27241 gcatgccgcc caccgacttg tgccccttga gcccggccag ccggcgggcc gccgcctcgg
    27301 cgaggaaatc cgcctccagg gccggatcgg ccagcgtgaa aggcacgttc atcagggacc
    27361 ggcagggacg atccaccggg ttgcggtaga agccgccgct gccgtcgatg gcgtcgtaga
    27421 gccgctctgc cttggcccgg ttggccgcct cgacggccgc cagtccgccc tggcggagca
    27481 gatccttgaa gatcagccca gccagataga tgccgaaggt gggcggtgtg ttgagcatgg
    27541 aaccatgctc ggccatggtg gcgaagttca tcacgctggg cgtggcggcg gaggcgcggc
    27601 cgaggagatc ctcgcgcacg atgaccagca ccagcccggc cgggccgatg ttcttctggg
    27661 ctccggcata gatcaggcca tagcgggaca cgtccatcgg gcgcgacagg atgtgggagc
    27721 tcatgtccgc caccagcggc acgccggcgg gcgtggcagg cagatcaaac atttcaacgc
    27781 cgtggatggt ctcgttggtg cacaggtgca cgtaggcggc gtccggatcg aagctcagtt
    27841 catccgctgc cggcagacgg gtgaagcggc cgtcggcttc ggtcgtgcca gccagacgca
    27901 cggggccgat gtgaggcgct tccttccagg ccttcttgga ccaggcgccg gtcaccacgt
    27961 aatcggcgga gcgcccggcc agcaggttca tgggaatctg cgcgaacatc tgggtggcgc
    28021 caccttgcag gaacagcacc ttgtagccgt ccgggatgcc catcagggcg cgcagatcgg
    28081 cctcggcggc ggcgatgacg gacatgaagt ccttgccccg gtggctcatt tccatgatgc
    28141 ccatgccggc gccgtgccag tcgaggagct cctcgcgcac ctgctcaagt accggggtgg
    28201 gcaatgcggc cggaccggcc gagaagttgt agatcctcat cgcgtctgct tccttgcttg
    28261 cggtgaatca ggcgtcggcc cgcgggccct cgccgtcctg ctcgtcctct tcgctttcga
    28321 cgatcttctg cacgccggac agggtgctgc cctcatccac agagatcagc gtcacgccct
    28381 gggtggcacg gcccatctcg cggatctcgg agacgcgggt gcgcaccagc acgccggcgg
    28441 tggtgatgag catgatctgc tccttctcgg tgaccagcgt ggccgccacc accttgccgt
    28501 tgcggtcgga ggtctggatc gcgatcatgc ccttggtgcc gcggccatgg cgggtgtatt
    28561 cggcaatggg cgtgcgcttg ccgtagccgt tctcggtagc ggtcagcaca ctgggcgatt
    28621 cgtcgccggc caccagcagg gcgatgaccg actggtcgtc ctccagcatc atgccgcgca
    28681 cgccgcgggc ggtgcggccc atggggcgca cgtcattctc gctgaagcgc actgccttgc
    28741 cggcgtcgga gaacagcatc acgtcgtgct ggccgtcggt gatggccacg ccgaccaggt
    28801 agtcgccctc gtccaaagcc acggcgatga tgccggcctt gcgcgggttg gagaagtcgg
    28861 tgagcggcgt cttcttgacc gtgccctggg ccgtggccat gaacacgaag tggccttcgt
    28921 cgaattcctt gaccggcagc accgcggtga tcttctcgcc ttcgagcagc gggaagagat
    28981 tgacgatggg cttgccgcgg gcgttgcggt tgccctccgg cacttcgtac accttgagcc
    29041 agtacagccg gccacggttc gagaagcaca ggatgtggtc gtgggtattg gccacgaaga
    29101 gctggtcgac gaaatcgtct tccttcaccg ccgccgcctg cttgccgcga ccaccgcgct
    29161 tctgggcgcg gtagtcggtg aggggctggc gcttgaagta gcccgtgtgg gacagggtca
    29221 cgaccatgtc ctggggcgcg atgaggtctt ctatattgat gtcggcggtg ttgagcacca
    29281 cttccgagcg ccgcggatca ccgaactgct gtttgatgag ggccagctcg tcgccgatga
    29341 tggcggtgat gcgttcgggc cgggcgagga tgtcgagcag gtcggcgatg gtctccatca
    29401 cttccttgta ctcggccacg atcttgtcct gctccaggcc ggtgagccgc tgcaggcgca
    29461 gctccaggat cgcctgggcc tgggcgtcgg agagccggta tccctgcgcc gacaggccga
    29521 attccggcgc gaggccagtc ggccggaagc tgtcggaggc cgcgcgggcc agcatctctt
    29581 ccaccagggg cgagcgccac tggcgcgcca tcagggattt ctttgcttcg ggcggcgtgg
    29641 gcgcggcctt gatgagggcg atgatctcgt ccacgttgga cagggccacc gccaggcctt
    29701 ccagaatgtg cccgcgctcc cgcgccttgc gcagttcgaa gacggtgcgg cgggtgatca
    29761 cctcgcgccg gtgggcgagg aagcattcca gcatctggcg caggtcaagc agacgcggct
    29821 tgccgtccac cagggccacc atgttcatgc caaaggtgtc ctggagctgg gtctgcttga
    29881 acaggttgtt cagcaccacg tccggcatct cgttgcgctt gagctcgatg accacgcgca
    29941 tgcccgactt gtcggattcg tcgcggatct cgctgatgcc ctcgatgcgc ttgtcgttca
    30001 ccagctcggc gatcttttcg agcaacgcct tcttgttaac ctggtagggc agttcgtcga
    30061 cgatgatcgc ctggcggtcg cccttgccga tgtcctcgaa gtgggtgcgc gcccgcatca
    30121 ccacccggcc gcggccggtg cggtagcctt cgtgcacgcc ggagaggccg tagatcaggg
    30181 ccgcggtggg gaaatccggc gccgggacga tcttgatcag ctcgtcgatg gagacgtccg
    30241 gctcctggag caggcgcaga caggcgtcta ccacctcgcc cagattatgg ggcgggatgt
    30301 tggtcgccat gcccaccgcg atgccgccgg aaccgttgat gagcaggttg gggatgcgcg
    30361 ccggcagcac cagcggctct ttttcggagc cgtcgtagtt ggggccgaag tcgacggttt
    30421 ccttgtcgat gtcagccagc agctcgtgac cgatcttggc catgcggatt tcggtgtacc
    30481 gcatggccgc cgcgccatcg ccatccaccg aaccgaagtt gccctgacca tccaccagca
    30541 tgtagcgcag ggaaaagtcc tgggccatgc gcacgatggt gtcgtacacc gactggtcac
    30601 cgtgggggtg atatttaccg atcacgtcac cgacgatacg ggcggacttc ttgtaggcct
    30661 tgttccagtc attgcccagc tcgtgcatgg caaacagcac gcggcgatgg accggcttga
    30721 ggccgtcccg cacgtcgggc agcgcccgcc cgacgatcac gctcatggcg taatccaggt
    30781 aggcgtggcg catctcgtct tcgagactga tggggagggt ttctttggcg aacgggatca
    30841 tggcaccgca gcggatcggc aatccgtcta aaagacgaaa agggtgaatt ctagcatggc
    30901 gcaccgtgcc ggccggcgcg tccgcaagcc tgcggaagat gcgatcctgc cgggagaagg
    30961 cgcgacccgg gcggcgattg tgatcggtga tggacgtatg ctctaatccg ccgcgcacac
    31021 cccacaagac gagcccccga gggctccgac catttcagga ggagaaacca gatgaacacc
    31081 gcattccgcg tggcgtccgc caccctgctc gccctcaccg gcctgactgc ccaggcccag
    31141 tcgaaggaca tcgtcgtcga cccgaagggc gacgtaccct acgccatcga ccagcgcaac
    31201 gtcgtcgtca agagcggcgc cgacctgtgc tggcgcaccg gcagctggac gccggccgcc
    31261 gccggcagcg tccaggccgg cgaactgcct gtcggctgcc agtgtgaccg ggacgtggtc
    31321 ggagccgaca tatgccagcc gcccgcggcc cgcggcgcgt ccggcgctcc tgcggcggcg
    31381 gtcgccgcac cggccgcagc cgtagccgcc ccccggacct gtgatttcag cgtcaccctt
    31441 aacgccgacc agtcctttgc ctttggcaag gccaccctca gccccgccgc ccgacgggcc
    31501 ctcgatgagg tggcgacccg cagcgcccag tgcgcctccg tgcgcgccct cctcatcacc
    31561 ggccacaccg accgcctcgg cacccaggct tacaatcaga agctctccga aaaacgcgcc
    31621 ggagccgtca gcagctatct cgccggcaaa gggctgaagg ccggggacgt ggagatcatg
    31681 ggcgcaggca agacccagcc catcaagggc tgcgacgatt ccctgggcca caagaagctc
    31741 gttgactgtc tggcgcccaa tcgccgcgtg accatcgacg tgaagggtcc ggccaagtaa
    31801 tctgcaccct gcaaggcccg cccccgccac cccgccttgt gcggggtttt tcatgcctgc
    31861 aaggaccctc catggccccc gtcgaaaccg ttgccgacat cgacctcctc atcgaagccc
    31921 gctggatcat tccggtccaa cccgcgggcc tcaccctcga acgccatgcc gttgcgatcc
    31981 acgagggccg catcctcgcg gtgctgccca tcgacaacgc ccgccggcag ttccgacccc
    32041 gcgagatccg cccgctgaca gaccacatcc tgatgcccgg cctcgtcaat ctgcacgtcc
    32101 atgcggccat gaccctgatg cgcggtctcg ccgacgacct ccccctgatg cgctggctgc
    32161 aggaagccat ctggcccgcg gagggccgtc acgtctcgcc gggcttcgtg cgcgacggca
    32221 gcctgctcgc ggccgccgag atgttgcggg gcggcatcac cacctgcaac gacatgtatt
    32281 tctttccgga ggccgccgcc gaagccttca gccgcgtcgg cctgcgcgcc atgctgggca
    32341 tcactgtgct ggactttccc acccagtacg ccgccgacgc cgacgactat ctgcgcaagg
    32401 gcctcgccgc ccgcgacgcc tggcgccacg accccctgat cggtttcacc ctcgcccccc
    32461 acgcaccgta caccgtctct gacagaacct tcgagcgcgt cggcggattg gccgaagagc
    32521 tggacctgcc catccacatc catctccatg aaaccgccac cgaagtggcc gacagcctgc
    32581 gcgaccacgg cctgcggccg attgcccgcc tggaccgcct ggggctcctc ggccctggcc
    32641 tgatcggggt ccacgccgtc catctgtcac cggaagacat cgaccgtctg gccacgcatc
    32701 attgttccat cgcccactgc cccacttcca acatgaaact ggcgagcggt gccgcgccca
    32761 tggcggcagt ggcggagcgc ggactgcgga tcggcctggg tagcgacggc acggccagca
    32821 acaaccgcct cgacctcttc cgcgagatgc atcaggccgc gctcctcgcc aagcttacgt
    32881 caggcgatgc cggcgcgctg cccgcccacc gtgtcctgcg catggccacc ctcgacggcg
    32941 ccgccgcgct gggcctggac gaccgcatcg gctccatcga accggggaag gccgccgacc
    33001 tgtgtgccgt gcgcctggac gacgtcgaca cgctgccctg ctacgacccc gcctcgcatc
    33061 tgatctacgt gctcgggcgg gagtccgttt cagacgtctg ggtggctggc cggcaggcgc
    33121 tcaaagacgg cactttgttg caaatatcca acactgaact gtctagcctt gcacggctat
    33181 ggcagaatag gctccgccaa tgaattggat atgctgcctg acttgatttt gcatcggtcg
    33241 gacagatcgg atgaatctcc ggttcaaccc ctttgccttg cgacgaggaa aacgatgatc
    33301 aaacaagcca aaaaacaact gctgatggcc gccgctctgg cgaccctcgg tctgactgct
    33361 tccgccagtt tcgcgaagga tgtcgtcgtc gatacgaagg gtgaagtgcc ctacgtgatc
    33421 gatggccgca acgtcgttgc ccgcagcggc gccgatctgt gctggcgcac cggctactgg
    33481 acccccgccg ccgcttccac caccatggct ggcgagttcc cggtcggctg tgaatgcgac
    33541 aaggatgtcg tggccaagga aaagtgcgaa ccgaaggctg ccgcgcctgc cgcgggtgcc
    33601 aagggcgccg ccccgatcgc cgctcccgcg ccggtctccg aaaaggtcaa gctggccgcc
    33661 gacaccctgt tcgacttcga caaggccgtc ctgctgcccg ctggcaagac caagctggat
    33721 gacctggctg acaaggccaa gggtctgaac ctcgaagtga tcctcgccgt cggccatacc
    33781 gaccgcatcg gtggtgacgc ctacaaccag aagctgtccg agaagcgcgc cgccgcggtc
    33841 aaggactatc tggtttccaa gggtgttgcg gccaaccgtg tctacaccga aggcaagggc
    33901 aagaagcagc ccgtgaccgg cgacaagtgc aagggcctgg gcaaggacaa cggcaagaac
    33961 aagaagctgg tcgagtgcct gcagccggat cgtcgcgtgg aaatcgaagt gatcggcacc
    34021 aaataatcac ttctcccgca cgtcctcaaa aagccctgcc ctgcagggct ttttgttttt
    34081 cgtccgccct cctcgcggca acggctgcac tacaattccg gtttctgatt gcccctgatt
    34141 ccgaccagcc tccatcatga acgccgatcc cgccgaacta cagaaattca gtgaacttgc
    34201 ccaccgctgg tgggacccgg aatccgaatt caagccactg caccagatca atcctctgcg
    34261 tctcgactgg atcgacgaga ccgtccatct cgccggcaaa caggtcattg acgtaggctg
    34321 cggcggcggc atcctgtctg aatccatggc cgcccgcggc gcccatgtca ccggaatcga
    34381 cctctccgac aaggccctgg gtgtcgcccg cctgcacctg tatgaatccg gccagaccgt
    34441 ggattaccgc cacatctcgg cggaagacat ggcccgcgag acaccgggcc agttcgatgc
    34501 cgtcacctgc atggagatgc ttgaacacgt tcccgatccg gccagcacca tccgcgcctg
    34561 caccgccctg gtcaaacccg gcggccacgt cttcttctcc accctcaacc gcaatccgaa
    34621 ggcctacctg atggccgtgg tgggtgccga gtacgtcctc aaactgctgc ccaagggcac
    34681 tcacgattac gccaagttca tcaagccatc cgagctctcc cgctatgtac ggaacgccgg
    34741 actggacgtg gaagagctca tcggcatgcg ctacaacccc ttcacccaga tctacgccct
    34801 cggtcgcgat acggacgtca attacctgat gcgcacccgg cgcaatggct gacgcggtct
    34861 tcttcgatct ggatggcacc cttgctgaca cggcgccgga tctggccgtc gcgctcaatc
    34921 agttgcgcgc cgagttggga ctaccgccct gtcgcttcga gtccctgcgc ccctttgtct
    34981 cccagggaac ccgcggcatg ctgagcgccg ggcttggaat tttgcccgac catgcggaat
    35041 acgcccccct gtttgaccgc ttcctgagcc tctacgccgg gaacctgtgc gtccacaccc
    35101 gtctcttcac aggcatggaa gttgtgctcg acgccctgga tgagcgcggg ctcccgtggg
    35161 gcgtcgtcac caacaaggcc gaacgcttcg cccgccccat catcaccgcc ctcggcctgg
    35221 acggacgctg cgcctgcgtc gtcggtggcg ataccctgac ccgccgcaag ccgtttcccg
    35281 atcccctcct ccatgccgcc gatctggcgc gcgttgcccc gacccgcagt ctttacgtcg
    35341 gcgacgatcc ccgcgacatc acagcaggcc gggcagccgg catgcaaact gtcggagcac
    35401 ggtacggtta tctgggctcc aatcctgcca tcgacacgtg gggcgccgat gccctcatct
    35461 cacacccgga ggaacttctc gcccacctct gatgtccatg atctgcgtgg tagaatcaca
    35521 cccgcagaat cgatgtttca tcaccgtggg gctgacctgg cttcgacgtg ggttgcaaag
    35581 cagatcaggg cataccgagg accagtcacc tcgtaaatcc actggaaatc aacaaacgcc
    35641 aacgacgagc gtttcgctct ggccgcttaa ggcctaagcc gctgcaccgg ttcgttcctg
    35701 ggccggattg ggggcaactc cattgcagcg tcattcacag gaactagcag agggtcgggt
    35761 tacttggccc cgcgcgaaat cttaggtgac tcgcccgtgg tcggcctgcc gactggctaa
    35821 accccgggtt aaattcaaat aggtcggcta agtatgtaga actgactgta gagggcttgc
    35881 ggacgggggt tcgattcccc ccagctccac caaaccatat accgaagtag tccgaagaag
    35941 gccggaaaca cctgagaaat caagggtttc ggcctttttc acgtccgcat tagtccgggg
    36001 aggtttattg caagccggta acggttggcg gtaacttaga cggtaacagg cacttaccgc
    36061 caaggagtta ccgtcatggc acttaccgac accgccatca agtccgcgaa gcccaccgac
    36121 aagcccgtca agatgacgga cggcgacggc ctctatctgt tgatccaccc caacggcggt
    36181 aagtggtggc gcttcgacta ccgctttgac agtaagcgca agacgctttc gatgggtgtc
    36241 tatcctgatg tgtccctgcg cgacgcccgc aaccggcgag acgccgcccg gaagcagatc
    36301 gccaacggca tcgatccggg cgaacaccgc aaggcccaca agagcgcccg cgccgatgca
    36361 gtcgccaaca gcttcgaggc tgtcgcccgc gagtggtaca gcaagatgcg gcccacctgg
    36421 acgcccgacc acgccgacaa gatcatcaag cgcttcgagc gggacgtgtt cccttggatc
    36481 ggcggcaagc cggtcgccga cctgaacgcc cccgaggtgc tgaccaccat tcgccggatc
    36541 gaggcccgcg gacggctgga aacagctcac cgtgcccttc aaagctgcgg gcaggtgttc
    36601 cgatatgccg tagccaccgg acgcgccgaa cgcgatccta cgggcgatct acgcggcgcc
    36661 ctgcccccga ccagggaaaa gcacatggct gccctgaccg accccgccga ggtgggcgcc
    36721 ctgctccgcg ccgtcgatgc cttccggggc accctgatcg tgcaatctgc cctgaatctg
    36781 tcggcgctgg tgttcgtccg ccccggcgag ttgcgacaag ccgaatggtc ggagatagat
    36841 ctggacaagg gcgagtggaa catccccgcc agccggatga agggctggac gcgcaaaggc
    36901 atcacgacgc cgcacctcgt gccccttgcg ccgcaagcgg tggcgatcct gcgcgagctg
    36961 caaccactca ccgcgcgagg cgcgcacgtg ttcgccggcc gcgatccgaa gaagccaatg
    37021 agcaacgcgg ccgtgaatgc cgccctgcgc cgcatgggct acgacacaaa gaccgagatg
    37081 accggccacg gcttccgggc gatggcccgg acgatcctac atgaagagct gggcgtggat
    37141 cgggatgtga tagagcacca gcttgcacac gcggtcccgg acgccttggg cacggcctac
    37201 aaccggacga agttcatcaa ggaacgccgg gtgatgatga cccgatgggc cgactatctg
    37261 gacaaactca aggtaggcgc agaagtagtg accatgcgag gcgcggcatg agcagcggag
    37321 aagactgaca ctatccgtgt ggcccgtggc ctgatccggt ggttacacct ggtaagggtc
    37381 gcccgagaaa gcgccaaccg gctccggtgt cgactatcga tatgaaaacg gtaggaaaag
    37441 ccctaggcgt tgcactccgg gcgatgccag atctccctga tgaaacccga cgtcggttag
    37501 ctctcgactt ggcctacgcg tggggttgtt accaagccat gactgaagtt gccccatttc
    37561 cgtggacacc tcatccttca ggaacaagga gtccacc
//
LOCUS       DAOQPA010000022        35466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_494_length_35466_cov_18.187936, whole genome shotgun sequence.
ACCESSION   DAOQPA010000022 DAOQPA010000000
VERSION     DAOQPA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 35466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35466
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_494_length_35466_cov_18.187936"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PKX01_05020"
     CDS             <1..986
                     /locus_tag="PKX01_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ75517.1"
                     /translation="NTLIYVRGKGIVLDEPSVVAIRTEGGPNAKKTILAVGMAAKQML
                     GKTPGNITAIRPMKDGVIADFTVTEQMLKQFIKKVHDTRLFSPSPRIIICVPCGSTQV
                     ERRAIRESALGAGASNVYLIEEPMAAAIGAGMPVADATGSMVVDIGGGTTEVGVISLG
                     GMVYAGSIRVGGDKFDEAIVNYIRRNYGMLIGETTAESIKKEIGSAFPGSEVKEMEVK
                     GRNLAEGIPRAFTISSNEILEALTEPLNQIVSAVKIALEQTPPELGADIAERGMVLTG
                     GGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGIALEKMDQLGSIFTSE"
     gene            1097..2011
                     /gene="mreC"
                     /locus_tag="PKX01_05025"
     CDS             1097..2011
                     /gene="mreC"
                     /locus_tag="PKX01_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMZ75518.1"
                     /translation="MVGHSPPPFFKRGPAPLAKLVFFGVLSLAMLVSDLNLRYLDAFR
                     QGLSVLAYPLQMAAATPADFVRNASDYFASLVRLQRENSELKRKQLAGAERLLRQDQL
                     EQENRHLRTLLDARERQPVKSVIAEILYTAKDPFSRKVILDKGAQAGIEAGQAVVDDL
                     GVIGQITRVFPMQAELTLLTDKDQAIPVMIVRNGLRAMLFGAGNGLMELRYLAANADV
                     QPGDKVVTSGLDGVFIAGLPVATVARVDRDQSQTFARIPCLPAAGVEQFGHVLVLGHR
                     ESVAPRPPEPDPLPGKAGKGRKARLAKE"
     gene            2015..2536
                     /gene="mreD"
                     /locus_tag="PKX01_05030"
     CDS             2015..2536
                     /gene="mreD"
                     /locus_tag="PKX01_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HMZ75519.1"
                     /translation="MQPTNRSSRILLPVKMWFVFLTLFIALFLNFIPTGHLPAVPDFV
                     ALVLAFWCIREPLRIGIGTGFVLGVLTDIGVGAAMGQYALAYVLLAYAANELSRRVLW
                     FTPVEQALHVLPLLFLSQVVMVVVRVIAGADFPGWWMFFGSFVGALLWIPLHYVLLLP
                     QYQPVERDENRPI"
     gene            2609..4513
                     /gene="mrdA"
                     /locus_tag="PKX01_05035"
     CDS             2609..4513
                     /gene="mrdA"
                     /locus_tag="PKX01_05035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ75520.1"
                     /translation="MKNVRTPEQDLARFRGRLAVAGVVALVCFGLLVARFFYLQVVRY
                     DYYSTRAEDNRISLVPIVPNRGLIVDRNGTVLARNYSAYTLEITPSRVADLEATIEAL
                     SKLVDIQPKDRKRLKKLMDESKSFESLPIRNRLSDEEVARFIAQRYRFPGVELKARLF
                     RDYPLGSFASHIIGYIGRINDRDLERIEDNDDTANYRGTDHIGKAGLEKSYERELHGV
                     TGFEEVEVDSGGRAIRSLRRNPATPGNNLILTVDLKLQEVAEKAFGNRRGALVAIEPG
                     SGGVLALVSVPTFDPNLFVDGIAPQDWDELNTSPDHPMVNRALNGAYPPGSTFKPFMA
                     LAALMSGKRTAAQAIADPGFFDFGGHHFRDDKPGGHGTVDMYRSIVQSCDTYYYRLAN
                     EMGIELIAATVGQFGFGARTGIDIEGESVGVLPSPEWKKRRFKKPEQQKWFGGETISV
                     GIGQGYNAYTMLQLAHATATVAAGGVMYKPHLVKFIENAKDGEKRVIEPEPIKTIPIR
                     PEHIETIRRAMVGVNREGTGARAFAGAPYAAAGKTGTAQVFSLKGAKYSAKIAERLRD
                     HALFIAFAPADKPTIALAVLVENGGFGAESAAPIARQVLDYYLLGKLPASVAPEYPGA
                     EGVPEERPVE"
     gene            4506..5648
                     /gene="rodA"
                     /locus_tag="PKX01_05040"
     CDS             4506..5648
                     /gene="rodA"
                     /locus_tag="PKX01_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMZ75521.1"
                     /translation="MSEFRFQPLRWIARLLAPLDGPLVTIVLAILCFSTIVMVSASPE
                     RLDTLALNIAVAFTVMWVAASVPPQRMMTIALPLYVLGVLLLIGVALFGDVSKGARRW
                     LNIGVARIQPSELMKIAMPLMLAWYFQSREGMIRVRDFFIAAALLLVPVGLIAKQPDL
                     GTAILVAAAGFYVLFFAGLTWRLIVPVAVVGIVSVAAIVATGDRICQPDVPWPGLRDY
                     QKHRICTLLDPTSDPLGKGFHIIQSTIAIGSGGVLGKGWGKGTQTHLDFLPERHTDFI
                     FAVLTEEFGLAGALILAVLYILLIFRGLFIAAGAPTLFSRLLAGALTLIFFTYAFVNM
                     GMVSGILPVVGVPLPFVSYGGTALVTLCLGVGMLMSIQRHRRRVGT"
     gene            5705..6700
                     /locus_tag="PKX01_05045"
     CDS             5705..6700
                     /locus_tag="PKX01_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMZ75522.1"
                     /translation="MTPEAIRRPRRGPCCAIAPVSALLLAFGALLAGTAHAAAFEGMF
                     PSETLHIAPPPDADDAPAAAAPVTYAAPSYALPDRRELAALSLYRERGIASYYGRPFH
                     GRKTSSGDLFDMYAMTAAHPTLPIPSYVRVTNLANGRSVVVRVNDRGPYHPGRAIDLS
                     YTAAFKLGYADQGQANVEIAQVLPEEVAFVQTRPVPPLRRAASRPAPAQVAAAAVPVP
                     AAAPPAPRTESTPVAAPIAPPVATPAVAAAPLADAASSPVFLQLGTFGSLVNAESFKT
                     FVEHELRSVKETVSLLASDGKYRLHLGPFPSAQEARSMAERIATTLKLKPSVVQR"
     gene            6854..7030
                     /locus_tag="PKX01_05050"
     CDS             6854..7030
                     /locus_tag="PKX01_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75523.1"
                     /translation="MPVRPAPQRPKYRPLRRPSSPSATLLDPDGLPDETDLTRIRVRM
                     AAARQQQQRAHVPH"
     gene            7711..7986
                     /locus_tag="PKX01_05055"
     CDS             7711..7986
                     /locus_tag="PKX01_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75524.1"
                     /translation="MTTEARETAVRIGTADIETVAREGVARALAARTQATELSADEID
                     AVSGGLTLSTIQLAQPTLVLKPGILAGPYPVDVLKGGVIAAATVQKY"
     gene            7999..8469
                     /locus_tag="PKX01_05060"
     CDS             7999..8469
                     /locus_tag="PKX01_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75525.1"
                     /translation="MATQPEILCPSAFPEWDGARLFAVVGGTPERPETAYLDQLQPVT
                     PELLELAGPVAPIEVFRFAAPCAERACQQFDGSTGRCQLARKTARIAPVVVHKLPRCG
                     IRANCRWWLQEGPSACKRCPQVATLNYAPSEAIRHAADPARPLDEVGTTAPLGT"
     gene            8495..9328
                     /locus_tag="PKX01_05065"
     CDS             8495..9328
                     /locus_tag="PKX01_05065"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ75526.1"
                     /translation="MSSTRKALCVGINQFKNYPSAALQGCVNDARDMASLLQQLLGFT
                     AADIAVLTDAQATKAAIMAQLKAMVDGAKAGKYSYLVFSLSSHGTQVPDLSGDEPDRA
                     DEAFCPHDLAQKGNQWDPAHIITDDELHDLFVQIPPTVLLEVYLDTCHSGTGLKGIDM
                     LLDRKPRYLPPPSLEAFRQIEGRRSRGIAQAIKEKGPATHNILWAACRPDQTSADAHI
                     AGGWHGAFTYFFTKEMRASKNKLSRAQILTKVRADLAAGGYTQVPQLEVDATTRQLAA
                     A"
     gene            9415..9621
                     /locus_tag="PKX01_05070"
     CDS             9415..9621
                     /locus_tag="PKX01_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75527.1"
                     /translation="MRTPPCPSAPVELSAAEIEAVSGGMGALAILAGAAAFVAAGALV
                     AAVAVTADNAANGPLAGAWYRFTK"
     gene            9687..10934
                     /locus_tag="PKX01_05075"
     CDS             9687..10934
                     /locus_tag="PKX01_05075"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ75528.1"
                     /translation="MRPALFRPEVLAQRPDGRHGEVLIAHPPALILLVALAVAFTAGL
                     AAYAWWGEYTRKEHVSGSLVPTRGLIRVFTPQAGTVLEKAVREGQPVRQGDVLLVLSS
                     ERASTATRETQAAQLAALRQRRDALRAEQAKQTEIDTLSGSAAADRAHGLVKELAEAR
                     AQLDLQRSRVAAAERTVARHEQLVAARFVAEATLQQKQEELLDQRARQAETQRIIAGL
                     ERDLATARAELASAGLRRANTASAITRQISELDQQLTETDSRRSVVITAPADGIVTTL
                     LAEPGQTATPGTPLLSLLPAGAELQAQLLVPTRAAGFIRTGQEVVLRYQAFPYQRFGH
                     HLGQVVEVGRSVIQPADAGQAGSVQEPVYRVTVRLPAQSVQAYGEAMALQAGMTLDAD
                     VRLDRRRLIEWLFDPLLSVTGRI"
     gene            10948..13083
                     /locus_tag="PKX01_05080"
     CDS             10948..13083
                     /locus_tag="PKX01_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HMZ75529.1"
                     /translation="MLDFGFASRTPLILQGESAECGLACLAMVAGHHGHRIDLASLRA
                     RHSLSQRGVTLADLMRIAGTLHLAPRPLRLEPEQLGQLRLPCVLHWDLNHFVVLIRLR
                     GDRASVHDPACGARTLRLAELSRHFTGVALELNPTDAFTPATERRRVRLGQLVGPLRG
                     LGGSVLQALVLALVLQLFALLAPFYMQWVVDQALVTQDRDLVTVLGVGFLLLALVNAG
                     VAALRGWVLMVLGTSLNLAMLTRLFRHLIRLPMTWFDKRHLGDVVSRFESLNSIQRTL
                     TGGFLEALVDGAMALLTLAMMLFYSLPLASVAIVAAAAYAGLRAALYQPQRRAAEAHI
                     VRGARQHSHFLETVRGIQTLRLHAHEEPRTVAWHNLAVDQFNAGIRSQRLGLLYQGLN
                     TLLFGIENVVTVWLGARLVLDTASGAGFSVGMLFAFIAYKNQFVQRMAAFIEKALELR
                     MLGLHAERVGDIALAAAEPDEPPASAAAAPLAGHLSVRNLAFRYGDTDPRVLDDVSFD
                     VRAGESVALVGPSGGGKTTLVKLLLGLMTPESGGIEVDGVPLARFGLRRYREQVASVM
                     QDDQLFAGSLADNIAFFDPQADPTRIEACARLAAVHDDIAAMPMGYHTLVGDMGTVLS
                     GGQKQRVLLARALYRRPRILVLDEATSHLDIARERCVNDAIRGLPLTRLIVAHRPETI
                     ASCDRVIVLTGGRVTNEMYAVAGGMGGAG"
     gene            complement(13162..13617)
                     /locus_tag="PKX01_05085"
     CDS             complement(13162..13617)
                     /locus_tag="PKX01_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75530.1"
                     /translation="MFTRISVVPAALAAAFGVTVLAGLTGCEHPKPPAPAASIPEPDL
                     KDVAKRFVDPSYAVGALVIGKNGEVLPVSAEGKRIQPCLLPKAPETPTAKAPEIATQG
                     EIQECPKARDTTILDVQSVAVVRHTGSTCVTVGGGVFMGRVMPVYQVCY"
     gene            complement(13653..16970)
                     /locus_tag="PKX01_05090"
     CDS             complement(13653..16970)
                     /locus_tag="PKX01_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ75531.1"
                     /translation="MAACAIAFAGAAHAACDQPAGRLAAIEGLVEVRAGVHGPWQPAG
                     LRQMLCPGDQVAVRGPGRAAIALAQDVLVRLDQNTTLTLPGSPTRADTELGLTGGVVH
                     VISRFRKRLGVLTPVVNALVDGTEFVVADGDGRARVSVAEGHVRAVTGAGELSLGAGE
                     ATDVADGAAPGPALVVSPLDAVRWAIHYPQVARPPADALAADPVLRTAVSLAEAGRFR
                     AALDTLGDGGPPAFRAALFLGLGRADEARGVLSAATPEAGVAAVRAVIQVARNDAEAL
                     ATARDAVRSDPASAPARLALSYALQADRQVPAALAAAESATALRPDDPVAWARRAELE
                     LALSRLAAGRESARRALALNPAVPRARALAAFAELLAGRTEAAAEAFDALLADDGSEP
                     LAHLGRALAHLRQGEVAAGRQELEVAVLLDPSNAELRSALGRAYLEEDRDKVAGDQFE
                     LARRLDPASPTPWYFDAFRKLRNRDFLAAVADGEEAVARNESRGVLRSGELLDQDRAA
                     RSASLGEAYREVGFYGPMQARAMDALADDPQSPAAHRLLADAYAGVPRFETARVSELL
                     QAELRQPIGQMPLPPQFVMPALPVLDGPRALSPDESTGLFARKPDHFAFSALGGNQDT
                     GAASLVAAHAWERGQIGVGAFDYRSRALVAGDADTHLSGTRLTGQFALTPASSVFAEL
                     RHTERVSGDQTLSLFDADLKRLRDGVNSDLARVAFRMSRGIGEELLLEASTQQIRDRS
                     SQSAHVDDPLNGSYDVLNGYSARLNARRFSAMYSVQRAESALIAGVTSFKLSGTADQN
                     FDLSIPGFGVFPVFASTFSTRAGRDAAFGYSRWRLNPVVTFLTGAEYTRYDEPTVTKA
                     ERFSGKIGLLLQPRTGTMVRAALFQGVKGSKYDREGLEPTQFNGFNQVFDDFNGTRWT
                     RGALAVDQHFAGGAALGAEVSSRHLDVPGLGCTGEACLAHWHEDLHRVYMALPFGTRV
                     AASAEWRYENSHINDDPRQLSMFPYRLRTDLVPIRLWVKAGTGSINLENWAVRQNARM
                     PDAFTGSDLVGHSAFSVMNLRYSMPLIGKDVVASLGASNVFDRRFRFQNADYNGDPKA
                     PLFYPRRTVLVQLTLRY"
     gene            complement(17145..19127)
                     /locus_tag="PKX01_05095"
     CDS             complement(17145..19127)
                     /locus_tag="PKX01_05095"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ75532.1"
                     /translation="MIRHLARSLRAAVLPLASAAAMGVVLLTSAWDWEEGAGLRLLYA
                     LRGIRPPPDRVMIIALDATSAGALGQSERPERWARSLHAVLVGLLAERGAAVVGFDVL
                     FERSREPAEDDQALAAAFRRAGNVMLVEQLTRETISAPDGTALGSVDRRVRALPLFLD
                     AVAGSGPFVLPKTPEGVFEFWDSLPSAGDQPSLPVLMAERIGAAPSPAARRRVLNLYG
                     PLGSVRTVSYARALALAADPQEGAVAFAGKAVLVGFAESNQSRQADAYRTPFTREDGV
                     DLPGVELLATALGNLLDGSGLTRPEDAATVPPLLAWAALLSLPWLGLGTRRAALVTAI
                     LGGVAVAGSAWAFAVLDLWLPVILAGLVAPAVAALLGIAWHIRAARRRERQLRRAVDL
                     GLTHKGSEALASLLRGREAGRRFHGVCLCSDIEGYTALAENLSPEATRDILNRYFAAF
                     IPVVQAHGGHVMDIVGDAVLSLWLADDAPDEACARARAAVLELHTLMNRAPAEGTLPT
                     RFGLHVGPVFLGELGATHHREIRAVGNIVNTASRIQSANKHLGTRILASAEVTAHGGP
                     GSARPLGRFVLAGQSAPVGLHELRCEALPADADRAFADGLAAWSGGAADWARERFAAT
                     LTLVPGDGPARFYAARCRERGALGADPDGIVVLGSK"
     gene            19157..19801
                     /locus_tag="PKX01_05100"
     CDS             19157..19801
                     /locus_tag="PKX01_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ75533.1"
                     /translation="MNYLDRVDLFAGLDEAQTQSLVDRSRLRSFVASTVVVNEGDEGG
                     SMYVVQSGSLKAYLTDKTGREVTLSLLDEGDYFGELALLDDAPRSASVAAVTRSELLQ
                     VPRSAFLALIEEHPASMQVVIRNLVARIRALSDNVRSLALEDVFRRVTRAFETLAVER
                     DGQRLIERRLTQQDVASLVGASREMINRVLRELVAGGYVEIEQHHIVLRRKLPT"
     gene            19912..20121
                     /locus_tag="PKX01_05105"
     CDS             19912..20121
                     /locus_tag="PKX01_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75534.1"
                     /translation="MCFVDSPISACNAVREMVLTDQTQRECAREHDCPPGRNCPLEGC
                     FAEISGQSEEATRREVAAAVAAHHA"
     gene            20123..20338
                     /locus_tag="PKX01_05110"
     CDS             20123..20338
                     /locus_tag="PKX01_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75535.1"
                     /translation="MANRTLGTRQKQVFRGPAKGPAGRVVPRNPLVLPAAQRKAGPHG
                     KSESAKRQAAERRLNRQLEKLAGEGDG"
     gene            complement(20359..21225)
                     /locus_tag="PKX01_05115"
     CDS             complement(20359..21225)
                     /locus_tag="PKX01_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ75536.1"
                     /translation="MAPPKLKGLGRGLDALLSGNGDDAQTTEGELQTLPVDALKPGKY
                     QPRTRMDPGSLEELAASIKSQGVMQPILVRPVGSLFDAQRYEIIAGERRWRASRIAGL
                     EAVPCLVREIPDEAALAMSLIENIQREDLNPLEEAAGIQRLVDEFGLTHQQAADAVGR
                     SRPAASNLLRLLQLAQPVQELLMAGDIDMGHARALLPLPGATQVGLANVIAAKGLSVR
                     EAERLAYQALHPKPQSAAPAPDRDILRLEEEVADRLGATVKIKANKKGAGMVTIHFGT
                     LDQLDGLLERLR"
     gene            complement(21276..22046)
                     /locus_tag="PKX01_05120"
     CDS             complement(21276..22046)
                     /locus_tag="PKX01_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ75537.1"
                     /translation="MARIFCIANQKGGVGKTTTSVNLAAGLAAAGQRVLLIDLDPQGN
                     ATMGSGIDKRSLGRSVYHVLVGLATLAEARVASAAGKYDVLPANRDLAGAEVELVALP
                     RRENRLRDALGSHGDTYDFILIDCPPSLSLLTLNGLCCAHGVIIPMQCEYYALEGLSD
                     LVNSIKKVHANLNRDLKIIGLLRVMFDPRVTLQQQVSAQLEQHFGDKVFRTLVPRNVR
                     LAEAPSHGLPGVVFDRGSKGAQAYLAFAEEMIERIKTL"
     gene            complement(22050..22688)
                     /gene="rsmG"
                     /locus_tag="PKX01_05125"
     CDS             complement(22050..22688)
                     /gene="rsmG"
                     /locus_tag="PKX01_05125"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMZ75538.1"
                     /translation="MSMGRALADGIAALGLDLPADTPARLEAFARLLIKWNKVYNLTA
                     LRDEAQVVTHHLLDSLSVLPHLAGVTRLVDVGSGGGLPGVVLAIARPDLQVDSVETVQ
                     KKATFQNQARIELKLEHFRAHHARIESWQPPYGPDAAPDGIISRAFADLADFVNLTAH
                     LAGPDTRLFAMKGVYPADEIARLPAGFVLERCLELAVPGLDAARHLLIVKRT"
     gene            complement(22685..24589)
                     /gene="mnmG"
                     /locus_tag="PKX01_05130"
     CDS             complement(22685..24589)
                     /gene="mnmG"
                     /locus_tag="PKX01_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMZ75539.1"
                     /translation="MLYPTRFDVIVVGGGHAGTEAALAAARAGCATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQFRILNASKGPAVRATRAQADRV
                     LYKAAIRRRLENQPNLMLFQQAVDDLTVAGDRVTGVVTQIGLRFEAPTVVLTAGTFLN
                     GLIHVGLQNYSAGRAGDPPAIRLGQRLKELALPQGRLKTGTPPRLDARTIDFSVMQEQ
                     PGDDPVPVFSFLGHAGQHPHQLPCWITDTNPRTHDIIRANLDRSPMYSGVIEGVGPRY
                     CPSIEDKIHRFADKDSHHVFLEPEGLDTHEIYPNGISTSLPFDVQLAIVRSIRGLEQC
                     TILRPGYAIEYDYFDPRNLKASLETKSIGGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     LQVKGREAWCPRRDEAYLGVLVDDLITRGVSEPYRMFTSRAEYRLSLREDNADLRLTE
                     TGRQLGLVDDVRWAAFCTKREAIERETARLRATWATPAKVPAEAAEAVLGKAIEREYS
                     FFDLLRRPGVSYAALMGLPGAPEDLATDPQVVEQLEIAAKYQGYIDRQQDEVARQAGA
                     EATRLPADLDYTTVRGLSKEVQLRLNQHRPETIGQASRIQGITPAAISLLLVWLKRQD
                     LARDGERRSA"
     gene            24936..28691
                     /locus_tag="PKX01_05135"
     CDS             24936..28691
                     /locus_tag="PKX01_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ75540.1"
                     /translation="MKRGFTTHLVIWAIVLGLGAYLAGTLVHTRGAAQARARNTALTH
                     ARLVAEHAHASIRQVELILDDLAERLPRDALAQGTALPDIRRAALRDLLNTARGRMAG
                     IENVILSDSVGMLTVTAIDPVPMVAIDDRTYFRTVREGAPGQTFLSDAVQGRVSGKWG
                     LMVARRLEDASGRFLGLVAVNLGLDRQFIHFYRSLAPEGDAAIALWSHDWRLLTRYPV
                     VEPEIGKAHLIESLAPLIDADLNETSTVAVSPVDRTVRLIALRRVEDYPLYASFAVAE
                     SDYMAQWRDQARDTMLAILALLVGGGVLSTLEVRSGRTAERVRRLIRETPLGLITLRA
                     GAEGDLLLLQSNPAAHRILQTNLEPHAGERLVSLLPGLTGSPILERVRAVARQGQPWR
                     ASGLPYRDERLQALFDIVAFQVAPGVAGVMFDDVSEREAAVAALNARERDLRRAESLA
                     QLGSWRLRLADRSMTCSDECLRILGARAGMPVSVSTFTALIHPDDRERVRAAWRNLLA
                     GGRMDETYRIGREGRPTTWVRARAEHDPLPGDDITLLGTIQDITQQHEAEQRLKDSED
                     RFHQLFARHASIMLLIEPVSGAIVDANAAAAEFYGYAIETLVGMRIQDINALPPEDVE
                     RERQRAFKEQRNYFVFPHRLADGRLRTVEVYSSPIQAGGQTVLFSIMHDVTEKQLAES
                     RLRLAAGVFDNAHEGIVVTDLSGTILEVNRAYCTLTGYEREDLIGRNPRILQAGPSDE
                     AFYAEMWGHLGRRGFWRGDLHNRRKDGSYYLQETGITAVTNALGLRTHYIGFAEDVTL
                     LRQSQQRLEYLAYHDALTQLPNRLLLADHIRLALAAARREGRHVAVCYLDLDEFKPVN
                     DQWGHTVGDQLLVEVAGRLKACVRAEDTVARLGGDEFVILLASLDSRTEGEQALARVV
                     AALSSPFQLTVGEIGVGASIGVSVFPPDDADADSLIRHADQAMYLAKQAGRNSIRFFD
                     PDADRRARSLRDSVAEVALGLERGEFELYYQPQVHLRSGRVVGVEALLRWNHPQRGLL
                     APGGFLAEVDNTEVAVALGEWVLDTALRQADTWHRAGLELRVGINVSAHHLQQDTFAA
                     RLAERLSRHPELPAGRIELEILETAALEDLSRISRLMETCRRFGVSFALDDFGTGYAS
                     LAYLKHLPANLLKIDQSFIRDMLSDPEDHAIVQGIVGLAMAFRREVIAEGVESLAHGE
                     ALLALGCPLAQGYCIARPMPAAAVAPWVAHWCNPPEWSQPAPPEDRP"
     gene            28688..30685
                     /locus_tag="PKX01_05140"
     CDS             28688..30685
                     /locus_tag="PKX01_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ75541.1"
                     /translation="MKPLCPLPPPVAGLAPPLRACDPWLVVSADARRAALSEAAVRLL
                     ELPDAHAELPATIQTVLGQADGDFVLSLDGADGARFLSVALGPDPDARLGAGTRLAVL
                     HDASGLIRLQRALADSEGRLQAILGAAMDAIIAMDEAGRITLWNAAAERLFGWRADEV
                     RGRDLHETLAGTAERARFLAHAGRWTRGGTSPVFGQILRLPAHRRDGTALDIELSLGA
                     FRRHGHWQALGIVRDVSARVAAELELQVTGTRLQQALEGGNITYWDWDVSTGSLVLGP
                     RYFSMLGYPDDAFPHTAESFRQLVHPDDRPQVEAALKAVLTGQTAEFACDFRLRAADG
                     TDRWATGQGKVTARDAAGRPLRVIGTHRDIHAFHESEAALQRQLAETLRLHRELEEAQ
                     VQLVQSEKMAAIGQLAAGVAHEMNTPLGFVKSNVGTLQTYISPLLEVITALMAATDGL
                     PGGAALADARARAAALDLDFLRQDLPDLFAETRDGIVRVQRIVADLKDFSHVDSATWA
                     HADLHRGLDSTLNILHNDIKHRIQVIRDYGDLPLLWCRPSQLNQVFLNLLANAAQAIA
                     DRGTITVRTRADARHVTVDIADTGCGIAPEHLPRIFDAFFTTKPVGKGTGLGLSISGD
                     IVRKHGGHLEVSSRPGLGSTFRIVLPVQVPDAPPPEKVFPP"
     gene            30682..32028
                     /locus_tag="PKX01_05145"
     CDS             30682..32028
                     /locus_tag="PKX01_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75542.1"
                     /translation="MTAPPLSTAAMPPSPTTTATHTLLLVDDEPAILSALRRLFRLAG
                     YRILTAESGAEGLELLAAQAVDLVISDMRMPEMDGATFLAAVRQHSPETIRILLTGYA
                     DLSSTVAAINEGEIYRYVAKPWNDQDLTLLVHDALERRALLRENARLDAVTRAQNEEL
                     RALNAGLEAKVAERTAALSAALGRADEAHRQLRQSFTAMVRVFSELSEAGTASAGHGR
                     RVAELARSIARQLQLDDATQQTVMLAGLLHDLGKVGLSAELLAKSRARLSADERTVLM
                     HHPKRAESLLTGIPSLRDVARLIRHHHESFDGSGFPDRLVGLAIPLGSRILCVANDYD
                     GLLRGTSVAGRPLSHRETVEFMLANRGKRYDPPVVEALVNVVAGSEAVADDLAVNTAQ
                     LRVGMVLSADLVHPEGYLLLARDFPLDAAMIAQLQRLETSEGLRLQLRVRRGDDAR"
     gene            32025..33473
                     /locus_tag="PKX01_05150"
     CDS             32025..33473
                     /locus_tag="PKX01_05150"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HMZ75543.1"
                     /translation="MTRADGTLAPFQWASYFETGCADVDAQHRKLVDLVNALAARAAG
                     SGALNAAELGHILDGLGQYARHHFATEEALMERAGLDPRHVDAHRAAHADFVEQVGAL
                     AGAGESDNALPRLYEFVCAWLTYHILDTDQAMARQLRARAAGVEAAAAFDAEGAGTAD
                     PGNAALIVAVRSLLGVIAGRNAALARANADLERRVEARTAELATAQARLREADTLAAV
                     GQLAAGVAHEINNPIGFVTSNLSTLATYAEQLLTLTAAADGLAARHATDSAWQAARQT
                     VDVDFIRDDLSALLDDASRGLERVREIVQALQAFAAPSEVRFTPIALAPLLADLCRSV
                     PTAAGQTVRWETCDTPPPVAGSAPQLREAFRALLDNGVKALAGQPGTVSVRCTSETAA
                     IRVDVEDDGCGMAPSVRAHIFEPFFTTRPVGSGRGLGLSSAYRIFRQHGGRIEVRSAP
                     GQGSTFQVWLPRLPAPDAAAPAEHEKARPEPG"
     gene            complement(33496..34344)
                     /gene="htpX"
                     /locus_tag="PKX01_05155"
     CDS             complement(33496..34344)
                     /gene="htpX"
                     /locus_tag="PKX01_05155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HMZ75544.1"
                     /translation="MFGNWLKTSILMAGIIALFGVVGGLIGGQQGMLLALVFGGAMNV
                     FSYWFSDKMVLRMYNAQEVDEASSPYLYNMVRELAGRASLPMPRVYIIHEDQPNAFAT
                     GRNPDNAAVAATTGILQMLSARELRGVMAHELAHVQHRDILISTISATLAGAISALAN
                     FAMFFGGRDEEGRPANPIASIAVALLAPLAASLIQMAISRAREFEADRGGAQISGDPN
                     ALADALLKIDAFARGIPMPTAEAHPETGQMMIMNPLSGHGLAGLFSTHPATEERVARL
                     RSLATR"
     gene            complement(34463..35386)
                     /gene="fmt"
                     /locus_tag="PKX01_05160"
     CDS             complement(34463..35386)
                     /gene="fmt"
                     /locus_tag="PKX01_05160"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMZ75545.1"
                     /translation="MKVAFAGTPDFAAIALEAILAAGFEVPLVLTQPDRPAGRGMQLQ
                     PSPVKQVALAAGIPVHQPEKLRTPEQQAPLAACAADVLVVAAYGLILPQAVLDLPRHG
                     CLNIHASLLPRWRGAAPIHRALQAGDAETGITIMQMDAGLDTGPMLLKRAESIQPDDT
                     TASLHDRLAWLGSEMIVEALEALPGGTLTATPQPAEGVTYAAKIGKAEAVVDWTRPAA
                     EIERAIRAFNPFPGAFAPFQGTPVKLWRARAIDARGTPGEVLLAEGAGVIVACGEGAL
                     CITELQKPGGRRLSAADFLRGAPIAAGSRFD"
     gene            complement(35386..>35466)
                     /locus_tag="PKX01_05165"
     CDS             complement(35386..>35466)
                     /locus_tag="PKX01_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ75546.1"
                     /translation="VFVEYLSLLKQTRIKSKLAKKARITA"
BASE COUNT         5565 a        12824 c        11766 g         5311 t
ORIGIN      
        1 ccaacaccct gatctacgtg cgcggcaagg gcatcgtcct cgatgaaccg tccgtcgtgg
       61 cgatccggac cgaaggcgga ccgaatgcca agaagaccat cctcgccgtg ggcatggccg
      121 ccaagcagat gctgggcaag acgccgggca acatcaccgc catccgcccc atgaaggacg
      181 gcgtgatcgc cgacttcacc gtcaccgagc agatgctcaa gcagttcatc aagaaggtcc
      241 acgacacccg cctgttctcc cccagcccgc gcatcatcat ctgcgtgccc tgcggctcga
      301 cccaggtgga acgccgcgcc atccgcgaat ccgctctggg cgccggcgcc agcaacgtgt
      361 acctgatcga ggagcccatg gccgccgcca tcggcgccgg catgccggtg gcggatgcaa
      421 ccggctccat ggtcgtcgac atcggcggcg gcaccaccga agtcggcgtg atctccctgg
      481 gcggcatggt gtacgccggc tcgatccgcg tcggcggcga caagttcgac gaagccatcg
      541 tcaattacat ccgccgcaac tacggcatgc tgatcggcga gaccaccgcc gaatccatca
      601 agaaggaaat cggctcggcc ttccccggct ccgaggtcaa ggagatggaa gtcaagggcc
      661 gcaacctggc cgagggcatc ccccgcgcct tcaccatctc ctccaacgag atcctcgaag
      721 ccctcaccga gcccctcaac cagatcgtct cggcggtcaa gatcgccctg gaacagacgc
      781 cgcccgagct gggcgccgac atcgccgagc gcggcatggt cctcaccggt ggcggcgccc
      841 tgctgcgcga cctggaccgc ctgctgatgg aagagaccgg cctgccggtg atcgtcgccg
      901 aagacccgct cacctgcgtg gtgcgcggct cgggcatcgc cctcgaaaag atggaccagc
      961 tcggttccat cttcaccagc gaataaaccg cgtcagggcg gccgggacgt acgcggtgaa
     1021 cgcctcgttc gaccgtgcgc gtccgtcatc cgggtttgtc ccggcggtcg tcgtgcactg
     1081 accggacggg cagaccatgg tcggccattc gcctcctccc ttcttcaagc gcggtcccgc
     1141 gccgctggcc aagctcgtgt ttttcggcgt gctgtccctg gccatgctgg tgtccgacct
     1201 caacctacgc tacctggacg ccttccgcca gggcctgtcg gtgctcgcct atccgctgca
     1261 gatggccgcc gccacgccgg ccgacttcgt ccgcaacgcg tccgactact tcgcctcgct
     1321 ggtgcgcctg caacgcgaga acagcgaact caagcgcaag cagctggccg gcgccgagcg
     1381 tctgctgcgc caggaccagc tcgaacagga gaaccgccac ctgcgcaccc tcctcgacgc
     1441 ccgcgagcgc cagccggtga agtccgtcat tgccgagatt ctgtacaccg ccaaggatcc
     1501 cttctcccgc aaggtgatcc tcgacaaagg cgcccaggcg ggcatcgagg cgggtcaggc
     1561 ggtggtggat gacctgggcg tgatcggcca gatcacccgc gtcttcccga tgcaggcaga
     1621 gctcaccctg ctcaccgaca aggaccaggc gatccccgtg atgatcgtgc gcaacggtct
     1681 gcgggcgatg ctcttcggtg ccggcaacgg cctcatggag ctgcgctatc tggccgccaa
     1741 cgccgacgtg cagcccggcg acaaggtggt cacctccggc ctcgacggcg tcttcatcgc
     1801 cggactgccg gtggccaccg tggcgcgggt cgatcgtgac cagtcccaga ccttcgcccg
     1861 catcccctgc ctgccggcgg ccggcgtgga acagttcggc cacgtgctgg tgctcggcca
     1921 ccgggaaagc gtggcgccgc gcccgcccga acccgacccg ctgccgggca aggccggcaa
     1981 gggccgcaag gcgcgcctcg ccaaggagta agccgtgcag ccgaccaacc gttcgagccg
     2041 aatcctgttg ccggtgaaga tgtggttcgt cttcctgacc ctcttcatcg ccctgttcct
     2101 gaacttcatc cccaccggcc atctgccggc ggtgccggat ttcgtcgccc tggtgctggc
     2161 cttctggtgc atccgtgagc cgctgcgcat cggtatcggc accggcttcg tgctgggcgt
     2221 gctcaccgac atcggcgtcg gcgcggcaat ggggcagtac gccctggcct atgtgctgct
     2281 ggcttacgcg gccaacgagc tgtcgcggcg ggtcttgtgg ttcacgccgg tggagcaggc
     2341 cctccacgtc ctcccgctgc tgttcctcag ccaggtggtg atggtggtgg tgcgggtcat
     2401 cgccggtgcg gacttcccag gctggtggat gttcttcggc agcttcgtcg gcgccctgct
     2461 gtggattccc ctgcactacg tgctgctgct gccccagtac cagcccgtcg agcgggacga
     2521 gaaccggccg atctgacgga gctccggcga tgcgcgcagg ccccttcctc ccgctgcatg
     2581 cgggccgcca tcccgagcgg caacgcccat gaagaacgtg cgcacccccg agcaggatct
     2641 cgcccgcttc cgcggccgcc tcgcagtggc cggcgtggtg gcgctggtgt gcttcggtct
     2701 gctggtggca cgcttcttct acctgcaggt ggtgcgttac gattactact ccacccgcgc
     2761 cgaagacaac cgcatctccc tggtgcccat cgtgcccaac cggggcctca tcgtggatcg
     2821 caacggcacc gtgctggcgc gcaactactc ggcctacacg ctggagatca cgccgtcccg
     2881 ggtagccgac ctggaagcca ccatcgaggc cctctccaag ctggtggaca tccagcccaa
     2941 ggaccgcaag cgcctcaaga agctgatgga cgagtccaag agtttcgagt cgctgcccat
     3001 ccgcaaccgc ctgtccgacg aagaggtggc ccgcttcatc gcccagcgct accgctttcc
     3061 cggcgtcgag ctgaaggcgc gcctgttccg cgactacccg ctgggcagct ttgcgtcgca
     3121 catcatcggc tacatcgggc gcatcaacga ccgcgacctg gaacgcatcg aggacaacga
     3181 cgacaccgcc aactaccggg gcaccgacca catcggcaag gccggcctgg agaagagtta
     3241 cgagcgcgaa ctgcacggcg tcaccggctt cgaggaggtt gaggtggatt ccggcgggcg
     3301 ggcgatccgc tccctgcgcc gcaaccccgc cacgccgggc aacaacctga tcctgaccgt
     3361 ggacctcaaa ctccaggaag tggccgagaa ggctttcggc aaccgccgcg gcgccctggt
     3421 ggccatcgag cccgggagcg gcggcgtgct ggcgctggtg tcggtgccca ccttcgatcc
     3481 caacctgttc gtggacggca tcgcccccca ggactgggac gagctcaaca cctcgcccga
     3541 tcaccccatg gtgaatcgcg ccctcaacgg cgcctacccg cccggctcca ccttcaagcc
     3601 cttcatggcc ctggcagccc tgatgagcgg caagcgcacc gccgcccagg ccatcgccga
     3661 cccgggcttc ttcgacttcg gcggccacca cttccgcgac gacaagccgg gtggccatgg
     3721 caccgtggac atgtaccgct ccatcgtgca gtcctgcgat acctactact accgcctcgc
     3781 caacgagatg ggcatcgaac tgattgccgc caccgtcggc cagtttggct tcggcgcccg
     3841 aaccggcatc gacatcgagg gcgaatccgt cggcgtgctg ccctcacccg aatggaagaa
     3901 gcgccgcttc aagaagcccg agcagcagaa gtggttcggc ggcgaaacca tctcggtggg
     3961 catcggtcag ggctacaacg cctacaccat gctccaactc gcccacgcca ccgccaccgt
     4021 ggcggccggc ggcgtgatgt acaagcccca cctggtgaag ttcatcgaga acgccaagga
     4081 cggcgaaaaa cgcgtcatcg agccggagcc gatcaagacc attccgatcc gccccgagca
     4141 catcgagacg atccgccgcg ccatggtcgg cgtgaaccgc gaaggcaccg gcgcccgcgc
     4201 ctttgccggc gcgccctacg cggccgcagg caagaccggc accgcccagg tgttctccct
     4261 gaagggcgcc aagtattccg ccaagatcgc cgagcgcctg cgcgaccacg ccctcttcat
     4321 cgccttcgcc ccggccgaca agcccaccat cgcgctggcc gtgctggtgg agaacggtgg
     4381 cttcggcgcc gagtccgccg cccccatcgc ccgccaggtg ctggactact acctgctcgg
     4441 caagctgcca gccagcgtgg cgcccgaata tccgggcgcc gaaggcgtgc cggaggagcg
     4501 ccccgttgag tgaattccgc ttccagcccc tgcgctggat cgcccgtctg ctggcgcccc
     4561 tcgacgggcc gctggtgacc atcgtcctgg cgatcctgtg cttctcgacg atcgtgatgg
     4621 tcagcgcatc gccggaacgc ctggacaccc tggccctcaa catcgccgtg gccttcacgg
     4681 tgatgtgggt ggcggccagc gtgccccccc agcgcatgat gaccatcgcc ctgcccctct
     4741 acgtgctggg cgtactgctg cttatcggcg tcgccctgtt cggcgacgtc tccaagggag
     4801 cgcgacgctg gctcaacatc ggcgtggcgc gcatccagcc ctcggaactg atgaagatcg
     4861 ccatgcccct gatgctcgcc tggtacttcc agagccgcga aggcatgatc cgcgtgcgcg
     4921 acttcttcat cgccgcggcc ctgctgctgg tgccggtcgg cctcatcgcc aagcagcccg
     4981 acctgggcac cgccatcctg gtggcggcgg cgggttttta cgtgctgttc ttcgccggcc
     5041 tcacctggcg cctgatcgta ccggtggccg tggtgggcat cgtcagcgtc gccgccatcg
     5101 tcgccaccgg cgaccggatc tgtcagcccg acgtaccctg gcccggtctg cgggactacc
     5161 agaagcaccg catctgcacg ctgctcgatc ccacctccga ccccctcggc aagggcttcc
     5221 acatcatcca gtccaccatc gccatcggct ccggcggcgt actgggcaag ggctggggca
     5281 agggcaccca gacgcacctg gacttcctgc ccgaaagaca cacggatttt atcttcgcgg
     5341 tgctcaccga ggaatttgga ctagccggcg ccctgattct tgcggtactc tacatcctgt
     5401 tgattttccg cgggcttttc atcgccgcgg gtgccccgac cctgttctcg cgccttctcg
     5461 ccggcgccct gacgctgatc ttcttcacct acgcgttcgt gaacatgggc atggtgagcg
     5521 gcattctccc cgtcgtgggc gtgccgcttc ccttcgtcag ttacggtggc acagccctcg
     5581 tcaccctgtg tctcggtgtg gggatgctca tgagcatcca gcgacaccga cgccgggtgg
     5641 gtacctgaac accgcgcagc accgggcctt gcggccggct gcgccctgac aggaaacgtg
     5701 catcatgacc cctgaagcca tccgccgccc ccggcgaggg ccctgctgcg ccatcgctcc
     5761 ggtgtccgcc ctgctgctgg ccttcggcgc cttgctggcc ggtacagccc atgccgccgc
     5821 cttcgaaggc atgttcccca gcgagacgct ccacatcgcc ccgccgcccg atgcggacga
     5881 tgcgcccgcc gctgccgcgc cggtcaccta tgccgcgccg tcctacgccc tgccggaccg
     5941 gcgcgaactc gccgccctgt ccctttaccg ggagcgcggc atcgccagtt actacggccg
     6001 ccccttccat gggcgcaaga cctccagcgg cgacctcttc gacatgtacg cgatgaccgc
     6061 cgcccacccg acgctgccca tccccagcta cgtgcgggtc accaacctgg ccaacggccg
     6121 ctcggtggtc gtccgcgtga atgaccgggg gccctaccat ccgggacgtg ccatcgacct
     6181 ctcctacact gccgccttca agctcggcta cgcggaccag ggtcaggcca acgtggagat
     6241 cgcccaggtc ctgcccgagg aagtcgcctt cgtccagacc cgcccggtcc cgccgctgcg
     6301 ccgtgctgcc agccgacctg cgccggccca ggtcgccgcc gcggccgtgc ccgtgccggc
     6361 ggctgcgccg cctgccccac gtaccgaaag cacgccggtc gctgcgccca tcgccccgcc
     6421 ggttgccacg cctgcggtcg ctgccgcgcc cctggccgac gctgcgtcca gcccggtctt
     6481 tctgcagctg gggaccttcg gaagccttgt aaacgccgaa tccttcaaga ccttcgtcga
     6541 acacgagctg cgcagcgtga aggaaaccgt ctccctgctc gccagcgacg gcaagtaccg
     6601 ccttcacctc ggcccctttc ccagcgccca ggaagcacgc tccatggccg agcgcatcgc
     6661 caccaccctc aagctcaaac ccagcgtcgt ccaacgctga tccccgtccg gacgtgtgat
     6721 caaattcaca cgaatccagt gacgcatgcc gactcgcgat cccggccgga tcgctaagct
     6781 atcggcgtgg gctggaatcc cggccgcccg cctccgaacg cccgggacaa cctcaacccg
     6841 gaaggaggat cccatgcccg tgcgtcccgc cccccagcgt cccaagtacc gccccctgcg
     6901 ccgcccgtcc tcgccgtcgg ccaccctcct cgatccagac ggactccccg acgaaaccga
     6961 cctgacgcgc atccgcgtcc gcatggccgc cgcccggcag cagcagcagc gggcccacgt
     7021 gccccactga atccaccgaa cccgcgctcg gatggcgcga gccctcttcg ggctcctgcc
     7081 cgtcgtgccg ggggcacgcc caggcgaggc gtgccggcag tgacgggcgg acgacccgcc
     7141 ggatcgcccc ctcatcgcgt ttctcccctc tcggccgcgc ccgcgcacaa acgcttccgg
     7201 ggacgccccc cgcgcccgga acgccagcac cgccccctca cagacagaca gcctccctgc
     7261 cggcctgcag cccccgcggg gcgatcctcc tccgcgaggg gccatcaccc agggacgcgt
     7321 gaccgcccct cgcgcgcgaa tgtccgcccc tcgcgtccga gggatcatcc ctcgcctacg
     7381 cgtgagcatc cctcgccgac gagtgaattt cactcgcacg cgaccgtctc ccacacgccg
     7441 acgagcgtgg ctcaccccgc agcgaatgac gctcatcggc tgacgagaac acttcagtcg
     7501 cacacggacg atttccaccc gaccgcgggt gagcgtcagg cctgcgcccc caaccgaccg
     7561 gccaaagtgt gaccaaattc acgcaaatcc tgtggcacat gacgtcgaac tgcatcggcc
     7621 atctgcgtaa tctgggaccc gtgatctgga acaacgggat gcccgccgga ccacacccgc
     7681 ccactcttcc actcgcagga gaacgccatc atgaccaccg aagcccgtga aactgctgtc
     7741 cgcatcggca ccgccgacat cgaaaccgtc gcccgcgaag gtgtcgcccg cgcgctggcc
     7801 gcccgcaccc aggccaccga actgagcgcc gacgagatcg acgccgtcag tggcggcctg
     7861 accctctcga cgatccagct cgcccaaccg accctggtcc tcaagccggg tatcctcgcc
     7921 ggcccgtacc cggtggatgt gctcaagggt ggcgtcatcg ccgcggccac cgtgcagaag
     7981 tactgagaga ccgccgccat ggccacccag cccgagatcc tgtgccccag cgcctttccc
     8041 gaatgggacg gggcacgctt gttcgcggtg gtcggcggca caccggaacg gccggaaacg
     8101 gcctacctcg accagctcca gccggtgacc ccggaactcc tggagctggc cgggccggtg
     8161 gccccgatcg aagtcttccg ttttgccgcc ccctgcgccg agcgcgcctg ccagcagttc
     8221 gacggctcca ccggccgctg tcagctggcc cgcaagacag cccggatcgc accggtggtg
     8281 gtgcacaagc tgccccgctg cggcatccgc gccaactgcc gctggtggct gcaggaaggc
     8341 ccctctgcat gcaaacgctg cccccaggtc gccacgctca actacgcgcc gagcgaggcc
     8401 atccgccatg cagccgatcc tgcccgcccc ctcgacgaag ttggcaccac cgcaccgctt
     8461 ggcacctgaa cccacccctt tccaggagac catcatgagc agcacccgca aagccctctg
     8521 cgtcggcatc aaccagttca agaactaccc ctccgcggcc cttcagggct gcgtcaatga
     8581 cgcccgcgac atggcgtccc tgcttcagca actgctcggc ttcaccgcgg ccgacatcgc
     8641 cgtgctgacc gacgcccagg ccaccaaggc cgccatcatg gcccagctca aagccatggt
     8701 ggacggtgcc aaggccggca agtacagcta cctggtgttc agtctctcca gtcatggcac
     8761 ccaggttccc gacctgagtg gcgatgaacc cgaccgcgcc gacgaggcct tctgccccca
     8821 cgatctggcc cagaagggca accagtggga cccggcccac atcatcaccg acgacgaact
     8881 ccacgacctc ttcgtgcaga tcccacccac cgtgctgctg gaggtctatc tggacacctg
     8941 ccacagcggc accggcctca aggggatcga catgctcctc gaccgcaagc cgcgctacct
     9001 gccgccgccg tccctggaag cctttcgcca gatcgaagga cgtcgcagcc gggggatcgc
     9061 ccaggccatc aaggaaaagg gcccggccac ccacaacatc ctgtgggcgg cctgccgtcc
     9121 cgaccagacc agcgccgacg cccacattgc cggcggctgg catggcgcct tcacctattt
     9181 cttcaccaag gagatgcgcg ccagcaagaa caagctcagc cgcgcccaga tcctgaccaa
     9241 ggtgcgggcc gatctggcgg ccggtggcta tacccaggtc ccgcagctgg aagtggacgc
     9301 caccacgcgg caactggccg cggcctgaca tcggcgcagt ccagcgtcgc ccgccgtccg
     9361 gcccacgcgg gcgacgcctc accctcaggg ctcattccat ccaggagttc catcatgcgt
     9421 acccctccct gtccgtccgc ccctgtcgaa ctgtccgccg ctgagattga agccgtatcc
     9481 ggcggcatgg gcgcacttgc catcctcgcg ggtgccgcgg cgttcgtcgc agccggggcc
     9541 cttgttgcgg cggtcgccgt caccgctgac aacgccgcca acggccccct cgcgggcgcc
     9601 tggtaccgct tcaccaagta agtccccgcg gccggggcgt cccccacgtt ccggccgcgt
     9661 cctcctgcct gctaccggag acggccatgc gccctgctct gttccgcccc gaagtcctgg
     9721 cccagcgccc ggacggccgc cacggcgagg tgctgatcgc ccatccgccg gccctgatcc
     9781 tgctggtcgc gctggccgtg gccttcaccg caggcctcgc cgcctacgcg tggtggggcg
     9841 agtacacccg caaggaacac gtcagcggca gcctcgtgcc cacccgtggt ctgatccgtg
     9901 tcttcacccc ccaggccggc acggtgctgg aaaaggccgt gcgcgagggc cagcctgtcc
     9961 ggcagggcga cgtcctgctg gtcctgagca gcgaacgggc aagcaccgcg acccgcgaga
    10021 cccaggccgc ccagctcgcc gccctgcgcc agcgccgcga cgccctgcgc gccgagcagg
    10081 ccaagcagac cgagatcgac accctgagcg gcagcgccgc agccgaccgc gcccacggcc
    10141 tggtgaagga acttgccgag gcccgcgccc agctcgatct gcaacgcagc cgggtggccg
    10201 ccgccgagcg cacggtggcg cgtcacgagc agctggtcgc ggcccgcttc gtcgctgaag
    10261 ccacgctcca gcagaagcag gaagagctcc tcgaccaacg cgcccgccag gccgagaccc
    10321 agcgcatcat cgccggactg gaacgggatc tcgccacggc gcgggccgag ttggccagcg
    10381 ccggcctccg gcgcgccaac accgcctcgg ccatcacccg ccagatctcg gagctggacc
    10441 agcagctcac cgagaccgac agccggcgca gcgtggtcat caccgcgcca gccgacggca
    10501 tagtcaccac gctcctcgcc gaacccggcc agaccgccac gccgggtacg ccgctgctgt
    10561 ccctgctgcc cgcgggtgcc gaactgcagg cacagctgct ggtgccgacc cgtgccgccg
    10621 gcttcatccg caccgggcag gaggtggtgc tgcgctacca ggcctttccc taccagcgct
    10681 tcggccatca cctggggcag gtggtggagg tcggccgcag cgtcatccag cccgccgacg
    10741 ccggtcaggc cggcagcgtg caggagccgg tctatcgtgt caccgtacgt ctgccggccc
    10801 agagcgtgca ggcctacggc gaggcgatgg ccctgcaggc gggcatgacc ctggatgccg
    10861 acgtacgtct cgaccggcgg cgcctgatcg agtggctgtt cgaccccctg ctgtccgtca
    10921 ccggccgtat ctgaggagat ccccgccatg ctcgacttcg gatttgcgtc gaggacgccc
    10981 ctgatcctgc agggggaatc cgccgaatgc ggactcgcct gccttgccat ggtggccggc
    11041 catcacggcc accgcattga cctcgcttcc ctgcgcgcgc gccacagcct gtcccagcgg
    11101 ggcgtcaccc tggccgacct gatgcgcatc gccggcaccc tgcatctggc cccacggccc
    11161 ctgcgcctgg agccggaaca gctcggacag ctgcgtctgc cctgtgtgct gcactgggac
    11221 ctcaaccatt ttgtcgtgct gatccgcctg cggggcgacc gggcgagcgt ccacgatccc
    11281 gcctgcgggg cgcgcacgct gcgcctggcc gagctttccc gccacttcac cggcgtcgcc
    11341 ctggaactca accccacgga cgccttcacg cccgccacgg aacgccgccg cgtccgcctc
    11401 ggccaactgg tcggccccct gcgcggattg ggcggcagcg tcctccaagc cctcgtcctc
    11461 gccctcgtgc tgcaactctt cgccctgctg gcccccttct acatgcagtg ggtggtggat
    11521 caggccctgg tcactcagga ccgggacctg gtcaccgtgc tgggcgtagg cttcctcctg
    11581 ctggcgctgg tcaatgccgg cgtggccgcc ctgcgcggct gggtgctgat ggtgcttggc
    11641 acctccctca atctggccat gctcacgcgc ctgttccggc atctgatccg cctgcccatg
    11701 acgtggttcg acaagcgcca cctgggtgac gtcgtgtcgc gcttcgaatc cctcaacagc
    11761 attcaacgca ccctcaccgg cggcttcctc gaagccctgg tggatggcgc catggcgctg
    11821 ctcaccctgg ccatgatgct cttctacagc ctgcccctgg ccagcgtggc aatcgtcgcg
    11881 gcagcggcct acgccgggct gcgggcggcc ttgtaccagc cccagcgacg tgccgccgag
    11941 gcgcacatcg tgcgcggagc ccgccagcac agccacttcc tggagacggt gcgcggcatc
    12001 cagaccctcc gcctgcacgc ccacgaggaa ccccgcacgg tggcctggca caatctggcg
    12061 gtggaccagt tcaatgccgg catccgcagc cagcgcctgg gcctgctcta ccaggggctc
    12121 aacacgctcc tgttcggtat cgagaacgtg gtcaccgtgt ggctgggcgc ccgcctggtg
    12181 ctcgacaccg ccagcggcgc gggcttttcg gtaggcatgc tgttcgcttt catcgcctac
    12241 aagaaccagt tcgtgcagcg catggcggcc ttcatcgaaa aggccctgga gctgcgcatg
    12301 ctcggcctgc acgccgaacg ggtcggcgac atcgccctcg ccgcggccga gcccgacgag
    12361 ccgccggcat cggcagcggc tgcgcccctc gcgggccatc tgagcgtgcg caacctggcg
    12421 ttccgttatg gcgacaccga tccgcgggtg ctggacgacg tgagcttcga cgtgcgcgcc
    12481 ggcgaatcgg tcgcgctggt cggcccttcc ggcggcggca agaccacgct ggtgaagctg
    12541 ctccttggtc tgatgacgcc cgagtcaggc gggatcgagg tggatggcgt gccgctggcc
    12601 cgcttcggcc tgcgccgtta ccgggaacag gtcgccagcg tgatgcagga cgaccagctc
    12661 ttcgccggct ccctcgccga caatatcgcc ttcttcgacc ctcaggccga ccccacccgc
    12721 atcgaggcct gtgcacgcct cgccgccgtt cacgacgaca tcgcagcgat gcccatgggg
    12781 tatcacacgc tggtcggcga catgggcacg gtgttgtcgg gcggccagaa gcagcgcgtg
    12841 ctcctcgccc gcgccctcta ccgccgtccg cgcatcctgg tcctcgacga agccaccagc
    12901 cacctggaca tcgcccgcga acgctgcgtc aacgacgcca tccgcggcct ccccctcacc
    12961 cgcctcatcg tcgcccaccg ccccgagacc atcgccagct gcgaccgggt gatcgtgctg
    13021 acgggcgggc gggtgacgaa cgagatgtat gcagtggcag gggggatggg gggtgcggga
    13081 taaaaccgcg ggaacgatca gatcaggctc cgccagagtc ctcgaacgct tgaacccgcc
    13141 gccgatcccc cttccattag cttagtaaca cacttgataa acaggcatga cacgtcccat
    13201 gaatacgccc ccaccaacgg tcacacaagt ggatcccgta tgccgcacga ccgccacaga
    13261 ctgaacgtcc aaaatggtcg tatcccgggc tttaggacac tcctggattt caccctgggt
    13321 ggcgatttct ggggctttcg cagtaggcgt ctcaggcgcc ttgggcaata ggcagggctg
    13381 aatccgtttg ccttcggcgc taacggggag cacttcgcca tttttgccaa tcaccaacgc
    13441 gccgactgca taggatgggt ccacgaaacg cttcgcaaca tccttcaggt cgggctcggg
    13501 gatcgaggcc gccggtgcag gaggcttggg atgctcacat ccggtcagac ctgcaagaac
    13561 ggtgactcca aacgccgccg caagggcggc cggtacaacg ctaatgcgtg taaacatggc
    13621 ttctaaactc catagaacaa aagattcttc aatcagtaac gcagggtcag ctggaccaat
    13681 accgtgcgtc gaggatagaa aagaggggcc tttggatccc cgttgtaatc agcattctga
    13741 aatctgaacc gacgatcaaa cacgttgctc gcccccaggc ttgcaacgac gtccttcccg
    13801 atcaagggca tggagtatct taggttcatg acagaaaaag cggaatgccc caccagatcg
    13861 gagcctgtga aggcatccgg catccgcgca ttttgccgga cagcccaatt ttccagatta
    13921 attgatccgg tacccgcctt gacccacaga cgaatcggca cgaggtccgt cctgagccga
    13981 taggggaaca tcgacaattg cctggggtcg tcattgatgt ggctattctc ataacgccat
    14041 tcggccgaag cggcgacccg ggtcccaaaa ggcagggcca tatagacgcg gtgcagatcc
    14101 tcatgccagt gagcgaggca cgcttcgcct gtacaaccca gtccagggac atcaaggtgc
    14161 ctcgacgaca cttccgcccc cagcgcagcg ccgcccgcga aatgctgatc gaccgccaag
    14221 gcgccacgcg tccagcgcgt tccgttgaaa tcgtcaaaga cctgattgaa cccgttgaat
    14281 tgagttggct ccagtccttc ccgatcatat ttggaaccct ttacaccttg aaatagcgcc
    14341 gcacgaacca ttgtcccggt cctaggctgt agcagcaggc cgatttttcc gctgaatctt
    14401 tcagccttgg tgactgtcgg ctcgtcgtag cgggtgtatt cggcgccggt cagaaatgtt
    14461 acgaccggat tgaggcgcca ccgactgtac ccaaaggctg catcgcggcc cgcccgcgtt
    14521 gaaaacgtgg acgcaaaaac cggaaagacc ccgaatccgg gaatcgagag atcgaagttc
    14581 tgatctgctg taccgctcaa cttgaaactg gtgactccgg caatcagggc actctcggct
    14641 cgttgaacgg aatacatcgc ggaaaaacgg cgtgcattca gtcgcgcaga ataaccattg
    14701 aggacgtcgt aagaaccatt cagggggtcg tccacatggg cactttgcga tgaccgatcg
    14761 cgaatctgct gagtgcttgc ctccagcagg agctcttccc caatgccccg tgacatacgg
    14821 aacgccacgc gcgcgaggtc cgaattgact ccgtcacgca gacgcttgag gtccgcatca
    14881 aacagactca gcgtctgatc cccgctcacc cgctccgtat gccgcagttc cgcaaacacg
    14941 ctgctggccg gcgtcagggc gaactggccg gtgaggcggg tgccggagag atgggtgtcg
    15001 gcgtcgccgg ccacgagggc gcggctgcgg tagtcgaagg cgccgacgcc gatctggccg
    15061 cgctcccagg catgggcggc gacgaggctg gccgcgccgg tgtcctgatt gccgccaagg
    15121 gcagaaaacg caaagtggtc gggtttgcgc gcaaacaggc cggtgctctc gtccggcgag
    15181 agcgcacggg ggccgtccag caccggcagg gcgggcatca caaactgggg cggcaggggc
    15241 atctggccga tgggctggcg cagctcggct tgcagcagct cgctgacgcg cgcggtctcg
    15301 aagcgcggca cgccggcata ggcgtcggcg agcaggcggt gggcggccgg gctctgggga
    15361 tcgtccgcca gggcatccat ggcacgggcc tgcatggggc cgtagaagcc gacctcgcgg
    15421 taggcttcac cgagactggc gctgcgggcg gcccggtcct ggtcgaggag ttcgccggaa
    15481 cgcagtacgc cgcgggactc gttgcgcgcg acggcctctt cgccatcggc aacggcggcg
    15541 aggaaatccc ggttgcgcag cttgcggaag gcgtcgaaat accagggtgt gggcgatgcg
    15601 gggtcgaggc ggcgggccag ctcgaactgg tcgccggcca ccttgtcccg atcctcttcc
    15661 aggtaggcac ggccgagggc gctgcgcagc tcggcgttgg agggatcgag gaggacggcc
    15721 acctcgagtt cctgccggcc cgcggccacc tcgccctggc gcaggtgggc cagggcgcga
    15781 cccagatggg cgaggggctc gctgccgtcg tccgcgagca gcgcgtcgaa ggcctccgcg
    15841 gcggcttcgg tgcgtccggc gaggagttct gcgaatgcgg ccagcgcccg ggcgcggggc
    15901 accgcgggat tgagggcgag ggcacggcgc gcggactcgc gaccggcggc gaggcgcgac
    15961 agggccagct ccagttcggc gcgacgcgcc caggcgaccg gatcgtcagg gcgcagggcg
    16021 gtggcggact cggcggcagc aagggccgcc ggcacctgac ggtcggcctg cagggcatag
    16081 ctgagcgcca gccgggccgg cgcggaggcc ggatcgctgc gcaccgcatc gcgggccgtg
    16141 gcaagggcct cggcgtcgtt gcgggccacc tggatgaccg cccggacggc ggccacgccg
    16201 gcctccgggg tggccgcgga cagcacgccg cgggcttcat ccgcccgacc gagcccaagg
    16261 aacagagcgg cccggaaggc gggcggtcca ccgtcaccca gagtgtcgag cgccgcccgg
    16321 aagcgccccg cctcggccag actcaccgcc gtgcggagga cgggatcggc cgccagtgcg
    16381 tcggctggcg ggcgcgccac ctggggatag tggatcgccc agcggaccgc gtcgagggga
    16441 ctgacgacca gcgccggacc aggcgcggcg ccgtcggcca catcggtcgc ctcgccagcg
    16501 ccgaggctaa gttcgccggc gcccgtcacc gcgcgcacgt ggccttccgc cacactgacc
    16561 cgggcccgtc catccccatc ggcgacgacg aactccgtgc catccacgag ggcgttgacg
    16621 acgggcgtca gcacacccag gcgtttgcgg aagcggctga tgacatggac cacgcccccc
    16681 gtgaggccga gctcggtgtc cgcgcgggtg ggggacccag gcaacgtgag ggtggtgttc
    16741 tgatcgaggc ggacgagcac gtcctgggcg agggcgatgg cggcgcgacc ggggccgcgg
    16801 acggccacct gatcgccggg acacagcatc tggcggagac cggcgggttg ccacggacca
    16861 tggacgccgg cgcggacctc gaccagacct tcgatggcgg cgagacgccc ggcgggttga
    16921 tcacaggccg cgtgcgccgc gcccgcaaag gcgattgcac aggccgccac gacccaggca
    16981 tgccgtccgc tccgttcatc cgatgcccgt cgaccgtgcc ccatgatatg ccccgccatg
    17041 gcaacgcctt gattcggaaa agttccgcgc gatgttatcg caaaaggtga cgacaaccat
    17101 gccgtccgtc ggggatttct gcgggatacc gcgatgccgc aggtctactt cgatccgagc
    17161 acgacgatgc cgtccggatc ggcgccgagg gcgccacgct cacggcagcg ggcggcatag
    17221 aaccgggccg ggccgtcccc cgggacgagg gtcagggtgg ccgcgaagcg ttcgcgcgcc
    17281 cagtccgcag cgcccccgga ccaggcggcg aggccgtccg caaaggcccg gtcggcgtcc
    17341 gcggggaggg cctcgcagcg cagttcgtgc agcccgaccg gcgcagactg tcccgccagc
    17401 acgaagcggc cgaggggccg cgcactgccc ggccccccgt gggcagtcac ctcggcagac
    17461 gccaggatgc gcgtgcccag atgcttgttg gcgctctgga tgcggctggc ggtattgacg
    17521 atgttgccga cggcgcggat ctcccggtgg tgggtggcgc ccagttcccc caggaaaacc
    17581 ggccccacat gcagaccgaa gcgggttggc agggtgcctt cggcgggcgc acggttcatc
    17641 agggtgtgga gctcgaggac cgccgcgcgg gcacgggcgc aggcctcgtc cggagcatca
    17701 tcggccagcc acagggacag caccgcatcg ccgacgatgt ccatgacgtg cccgccgtgg
    17761 gcctgcacga cggggatgaa ggccgcgaag tagcgattga ggatgtcccg tgtcgcctcg
    17821 gggctgaggt tttcggcgag cgccgtatag ccttcgatgt cactgcacag gcacacacca
    17881 tgaaacctgc gccccgcctc acggccccgc agcagggacg ccagggcctc gctgcccttg
    17941 tgggtgaggc ccagatccac ggcacgcctc agctgacgtt cgcgccgccg tgccgcgcgg
    18001 atgtgccacg cgatgcccag cagtgcggcg acagccggcg cgaccagccc ggccagaatc
    18061 accggcagcc acaggtcgag gaccgcaaag gcccaggccg accccgccac ggcgaccccg
    18121 cccaggatcg ccgtgaccag cgccgcgcga cgcgtcccca gccccagcca gggcagactg
    18181 aggagggccg cccaggcgag caggggcggc acggtggcgg cgtcctccgg ccgtgtcaga
    18241 cccgaaccgt ccagcaggtt gccgagtgcc gtggcgagga gctcgacgcc tggcagatcg
    18301 acgccatcct cgcgggtgaa gggcgtgcga taggcgtcgg cctggcggga ctggttggac
    18361 tcggcgaagc ccaccagcac ggccttgccg gcgaacgcca ctgcaccttc ctgcgggtcg
    18421 gcggccagcg ccagggcacg ggcataggag acggtgcgga cactgccgag gggaccgtag
    18481 agattgagga cgcgacggcg ggcggccggt gacggggccg ctccgatccg ttccgccatc
    18541 agcaccggaa gggacggctg gtcaccggcg ctgggcaggg aatcccagaa ttcgaagacc
    18601 ccctccggcg tcttgggcag gacgaagggg ccgctgccgg cgaccgcatc gaggaacagg
    18661 ggcagggcac gtacgcgccg gtccacgctg cccagcgccg tgccatccgg cgcgctgatg
    18721 gtttcgcggg tgagctgctc caccagcatg acgttgccgg cacggcggaa cgcggcggcc
    18781 agcgcctgat cgtcctcggc gggctcacgg gaacgttcga acagcacgtc gaagccgacc
    18841 accgccgcgc cccgctcggc cagcagcccg accagcaccg cgtgcaggct gcgcgcccag
    18901 cgctcggggc gctccgactg cccgagggcc ccggcggacg tggcgtcgag ggctataatc
    18961 atgacccggt ccgggggcgg acggatgccg cgcagggcgt acagcagacg caggccggcg
    19021 ccctcttccc agtcccaggc cgatgtgagc agcaccacgc ccatggccgc ggcggaggcc
    19081 aggggcagga cggcggcacg caaggaacgg gcgaggtggc ggatcacggc ggaatcatcg
    19141 agcggggaga agcattgtga actatctgga tcgggtcgat ctgttcgcgg gcctcgacga
    19201 ggcccagacg cagagtctgg tggatcgctc gcgtctgcgc agcttcgtgg cgagcacggt
    19261 ggtcgtcaac gagggcgacg agggcggcag catgtacgtg gtgcagagcg gctctttgaa
    19321 agcctatctc accgacaaga cgggacggga ggtcaccctg tccctgctcg acgaagggga
    19381 ctacttcggc gagctggccc tgctcgatga cgcgccgcgg tcagcgtcgg tggcggcggt
    19441 gacgcgcagc gagctgctgc aggtgccgcg ctccgccttc ctggcgctga tcgaggagca
    19501 cccggccagc atgcaggtgg tgatacgcaa tctggtggcg cgcatccgtg ccctgtcgga
    19561 caacgtccgc tccctcgccc tggaggacgt cttccggcgc gtcacgcggg ccttcgagac
    19621 cctcgcggtg gagcgcgacg ggcaacgcct catcgagcgc cgcctgaccc agcaggacgt
    19681 ggccagcctg gtgggcgcgt cgcgggagat gatcaaccgg gtgctgcggg agctggtggc
    19741 cggcggctac gtggagatcg aacagcatca catcgtgctg cggcgcaagc tgccgacctg
    19801 accgctgcgg agcgcccatt ccgggtactc tgaccggaaa aaccgcacgg tttgcatggt
    19861 catttttcac gggcggacta cactgaaata aacacgaccg gggagacgat catgtgcttc
    19921 gtagattcgc ccatcagtgc ctgcaacgcc gtccgcgaga tggtgctcac cgatcagacc
    19981 caacgggaat gcgcccgcga gcatgactgc ccgcccggtc gcaactgtcc gctggaaggc
    20041 tgttttgccg aaatctcggg ccagagcgag gaggccaccc gccgcgaagt ggccgcggcg
    20101 gtcgccgccc atcacgcctg agatggccaa ccggacgctt ggcacgcgcc agaaacaggt
    20161 gttccgcggc cccgccaagg gccccgcggg gcgggtggtg ccgcgtaacc cgctggtgct
    20221 gccggcggcc cagcgcaagg ccgggcccca tggcaagtcg gaaagcgcca agcggcaggc
    20281 ggccgagcga cgcctgaacc gccagctcga aaagctggcc ggggagggcg acggctgaac
    20341 agccgccaac gcgcgccgtc aacgcaggcg ttcgagcaga ccgtcaagct ggtccagggt
    20401 gccgaagtgg atggtcacca tcccggcacc ctttttattg gccttgattt tcacggtggc
    20461 gccgaggcgg tccgccacct cctcttccag acgcaggatg tcccggtccg gcgccggagc
    20521 ggcgctctgc ggcttggggt gcagcgcctg gtaggcgagg cgctcggctt cgcgcaccga
    20581 caggcccttg gcggcgatca cgttggcgag gccgacctgc gtggcgcccg gcagaggcaa
    20641 cagggcgcgg gcgtggccca tgtcaatgtc gccggccatc agcagttcct gcaccggctg
    20701 cgccagctga agcagccgca gcaggttgga ggcggccggg cgggagcggc ccaccgcgtc
    20761 ggcggcctgc tgatgggtca ggccgaactc gtccacgagg cgctggatgc cggcggcctc
    20821 ttcgagggga ttgaggtctt cccgctggat gttctcgatg agggacatgg ccagcgcggc
    20881 ctcgtccgga atctcccgca ccaggcaggg cacggcctcc aggccggcga tgcgcgaggc
    20941 gcgccagcgc cgctcgcccg cgatgatctc gtagcgctgg gcgtcgaaca ggctgcccac
    21001 cgggcgcacc aggatgggct gcatgacgcc ctgggacttg atggacgcgg ccagttcttc
    21061 cagcgagccg gggtccatgc gcgtgcgtgg ctggtacttg ccgggcttga gggcgtcgac
    21121 gggcagcgtc tgcagttcgc cttcggtcgt ctgggcgtca tcgccgttgc cggacagcag
    21181 ggcgtcgagg ccgcggccga gacctttgag tttgggaggt gccatggtgt cgttctcgct
    21241 ggaggcgccg ggcagaggcg ggcgccggtc cggtctcaga gggtcttgat gcgttcgatc
    21301 atctcttcgg cgaaggccag gtaggcctgg gcgcccttgc tgccgcggtc gaagacgacg
    21361 cccggcaggc cgtggctggg cgcctcggcg aggcgcacgt tgcgcggcac gagggtgcgg
    21421 aagaccttgt cgccgaaatg ctgttcaagc tgggccgaga cctgttgctg gagcgtcacg
    21481 cggggatcga acatgacccg cagcaggccg atgatcttga ggtcgcggtt gaggttggcg
    21541 tgcaccttct tgatggaatt gaccaggtcg gacaggcctt ccagcgcgta atactcgcac
    21601 tgcatcggga tgatcacgcc gtgggcgcag cacaggccgt tgagggtcag cagggacagg
    21661 gacggcgggc agtcgatgag gatgaagtcg taggtgtcgc cgtggctgcc gagggcgtcg
    21721 cgcaggcggt tctcgcggcg cggcagggcc accagctcca cctcggcgcc ggccagatcg
    21781 cggttggcgg gcagcacgtc gtacttgccc gcggcggagg ccacccgcgc ctcggccagc
    21841 gtggccaggc ccaccagcac gtgatacacg gagcgcccca gactgcgctt gtcgatgccg
    21901 ctgcccatcg tggcgttgcc ctgggggtcg aggtcgatga gcagcacccg ctgcccggcc
    21961 gcggccaggc cggcggccag attgacgctg gtggtggtct tgccgacgcc gcccttctgg
    22021 ttggcgatgc agaaaatgcg ggccatggtt caggtccgtt tcacgatgag gaggtggcgc
    22081 gccgcgtcga ggcccggcac ggcgagttcg aggcagcgtt cgaggacgaa gccggcgggc
    22141 agacgggcga tctcgtcggc aggatagacg cccttcatgg cgaacagacg cgtgtccggc
    22201 ccggccagat gggccgtgag attcacgaag tccgccagat cggcgaaggc gcgggagatg
    22261 atgccatccg gcgcggcgtc cggcccgtag ggcggctgcc aggactcgat gcgggcgtgg
    22321 tgcgcgcgga agtgctcgag cttcagttcg atgcgcgcct ggttctggaa ggtcgccttt
    22381 ttttgcacgg tctcgacgga atccacctgc agatccggcc gggcgatggc cagtacgacg
    22441 ccgggcagac cgccgccgga gcccacatcc accagccgcg tgacgccggc caggtggggc
    22501 agcacgctga gggaatccag caggtggtgg gtcacgacct gggcctcgtc tcgcagggcg
    22561 gtgaggttgt acaccttgtt ccacttgatg agcagacgcg cgaaggcttc caggcgggcc
    22621 ggcgtgtcgg cgggcaggtc gaggccgagg gcggcgatgc cgtcggcgag ggcgcgcccc
    22681 atgctcatgc ggagcgtcgt tcgccgtcgc gggcgagatc ctgacgtttg agccacacca
    22741 gcagcaggct gatggccgcc ggggtgatgc cctggatgcg gctggcctgg ccgatggtct
    22801 cgggccggtg ctgattgagg cgcaactgga cttccttgga caggccacgc accgtggtgt
    22861 agtccaggtc ggcgggtagg cgggtggcct cggcgccagc ctggcgggcc acttcgtcct
    22921 gctggcggtc gatgtagccc tggtatttgg cggcgatctc gagctgttcg accacctggg
    22981 gatcggtggc gaggtcctcc ggcgcgccgg gcaggcccat cagcgccgcg tagctcacgc
    23041 cggggcggcg cagcaggtcg aagaagctgt attcccgctc gatggccttg cccagcaccg
    23101 cctcggcggc ctcggccggc accttggccg gcgtcgccca ggtggcgcgc aggcgggcag
    23161 tctcccgctc gatggcctcg cgcttggtgc agaaggcggc ccagcggacg tcgtccacca
    23221 ggcccagctg gcggccggtc tcggtgagac gcaggtcggc gttgtcctcc cgcaggctga
    23281 ggcggtactc ggcgcgggag gtgaacatgc ggtagggctc ggagacgccg cgggtgatga
    23341 gatcgtccac cagcacgccc agataggctt cgtcgcgacg cgggcaccag gcctcccggc
    23401 ccttcacctg gagcgccgcg ttgaggccgg cgagcaggcc ctgggcggcg gcttcctcgt
    23461 agccggtggt gccgttgatc tggccggcga agaacagccc gccgatggac ttggtctcga
    23521 ggctggcctt gaggttgcgc gggtcgaagt agtcgtactc gatggcgtag ccggggcgca
    23581 ggatggtgca ctgctccagc ccgcggatgg agcgcacgat ggccagctgc acgtcgaagg
    23641 gcaggctggt ggagatcccg ttggggtaga tctcgtgggt gtccaggcct tcgggctcga
    23701 ggaagacgtg gtggctgtcc ttgtcggcga agcggtggat cttgtcttcg atgctcgggc
    23761 agtagcgcgg accgacgcct tcgatcacgc cggagtacat gggcgagcgg tccaggttgg
    23821 cgcggatgat gtcgtgggtg cgcgggttgg tgtcggtgat ccagcacggc agctggtgcg
    23881 gatgctggcc ggcgtggccg aggaagctga acaccggcac cgggtcgtcg ccgggctgct
    23941 cctgcatcac cgagaagtcg atggtgcggg catccaggcg cggtggcgtg ccggtcttga
    24001 ggcggccctg gggcagggcg agttccttga gacgctggcc caggcggatc gccggcgggt
    24061 cgcccgcacg gccggcggag taattctgca ggcccacgtg gatcaggccg ttgaggaagg
    24121 tgccggcagt gagcaccacg gtgggcgcct cgaagcgcag gccaatctgg gtgaccacgc
    24181 cggtaacccg gtcgcccgcc acggtgagat cgtccacggc ctgctggaag agcatcaggt
    24241 tcggctgatt ctccaggcgg cggcggatgg cggccttgta cagcacccga tccgcctggg
    24301 cgcgggtggc ccgcaccgcc ggccccttgc tggcgttgag gatgcggaac tggatgccgc
    24361 cctcgtcggt ggcggccgcc atggccccgc ccagggcgtc cacctccttg accaggtgcc
    24421 ccttgccgat gccgccgatg gacggattgc agctcatctg cccgagggtt tcgatgttgt
    24481 gggtcagcag cagggtggca cacccggccc gcgccgccgc cagggcggcc tcggtaccgg
    24541 cgtgaccgcc gccgacgacg atcacgtcga aacgggtggg atagagcatg gacaaagcgg
    24601 gatggggatt cgggggatcg gaatgatacg accttgcgct gcaaaaatac agcgccgcgg
    24661 cacggcctcc agaataaaat cagcggtttt acaacagctt agcactcatt cccggcacca
    24721 tgacgggggt ccggacacgc cccttgaggc cgctgagcag cgctcgatga cacgggcgca
    24781 gatgggcatt ccggacccca cggcggatcg gccggaccgg agcggctacg cctgcggaat
    24841 atcgacctat acctaaagtc actccgaccc ccgccgatta agccggaggg cgtacgtcaa
    24901 tcctgctggc gcccgccccc atctttctgc gggacatgaa acgcggcttc accacccatc
    24961 tcgtcatctg ggccatcgtg ctcggtctgg gcgcctacct ggcgggcacc ctcgtgcaca
    25021 cgcgcggcgc ggcgcaggcc cgcgcccgca acacggccct gacccatgcc cggctggtgg
    25081 ccgagcacgc ccacgcgagc atccggcagg ttgaactgat actcgacgat ctggccgagc
    25141 gcctgccgcg cgacgcgttg gcccagggca ccgccttgcc ggacatccgt cgtgccgccc
    25201 tgcgggatct cctgaacacg gcccgcggac gcatggcggg catcgaaaac gtgatcctgt
    25261 cggacagtgt cggcatgctg accgtcaccg ccatcgaccc cgtcccgatg gtcgccatcg
    25321 acgaccgcac gtatttccgc acggtccgcg agggtgcgcc ggggcagacc ttcctgtcgg
    25381 acgccgtcca ggggcgggtg tccggcaaat gggggctgat ggtggcccgt cgcctggaag
    25441 acgcgagcgg acgctttctc gggctcgtgg ccgtcaatct ggggctggac cgccagttca
    25501 tccacttcta tcgctcgctg gcgcccgagg gcgacgcggc aatcgccctg tggagccacg
    25561 actggcgcct cctcacccgc taccccgtcg tcgaaccgga gatcggcaag gctcacctga
    25621 tcgagtcgct ggcgcccctg atcgacgctg acctgaacga gacgtccacg gtcgccgtct
    25681 cgccggtgga tcgtacggtg cgcctgatcg ccctgcgccg ggtggaggac tatccgctgt
    25741 acgcgagctt tgcggtcgcc gaatcggact acatggccca gtggcgcgac caggcccgcg
    25801 acacgatgct ggcgatcctt gccctgctgg tcggtggcgg cgtgctcagc accctggagg
    25861 tccgcagcgg ccgcaccgcc gagcgggtgc gccgcctgat ccgcgaaacc ccgctcggac
    25921 tgatcaccct gcgggccggc gccgaaggcg atctgctcct gctccagtcc aacccggcgg
    25981 cccaccgcat cctccagacc aatctcgaac cccacgccgg cgagcgtctg gtgagcctgc
    26041 tgcccggtct gaccggttcg ccgatcctgg agcgggtgcg cgcagtggcc cgacagggtc
    26101 agccctggcg ggccagcggg ctgccctacc gggacgagcg cctgcaagca ctcttcgaca
    26161 tcgtggcgtt ccaggtggcg ccgggcgtcg ccggcgtgat gtttgacgac gtgtccgagc
    26221 gggaagcggc cgtggcggcc ctcaacgccc gcgaacgaga cctgcgccgt gccgaatccc
    26281 tcgcccagct ggggagctgg cgcctgcgcc tggccgaccg cagcatgacc tgctccgacg
    26341 aatgtctgcg catcctcggc gcccgcgccg gcatgccggt gagcgtgtcc accttcaccg
    26401 cgctgatcca cccggacgac cgggaacggg tccgcgcggc gtggcgcaac ctgctcgccg
    26461 gcgggcgcat ggacgagact tatcgcatcg gccgcgaggg ccggcccacc acctgggtgc
    26521 gtgcgcgggc cgaacacgac ccgctgcccg gcgacgacat caccctgctc ggcaccatcc
    26581 aggacatcac ccagcagcac gaggccgaac agcgcctgaa ggacagcgaa gaccgcttcc
    26641 accagctctt cgcccgtcac gcctcgatca tgctgctcat cgagccggtc agcggcgcca
    26701 ttgtcgatgc caacgcggcg gcggccgagt tctacggcta cgccatcgag acgctggtgg
    26761 gcatgcgcat ccaggacatc aatgccttgc cgccggaaga cgtagagcgg gaacgccagc
    26821 gggcgttcaa ggaacagcgc aactacttcg tctttccgca ccggctcgcc gacggacgcc
    26881 tgcgcacggt ggaggtgtat tcatcgccaa tccaggccgg cggccagacc gtgctgttct
    26941 ccatcatgca cgacgtcacc gagaagcagc tggccgaatc gcgcctgcgc ctcgccgccg
    27001 gggtgttcga caatgcccat gaaggcatcg tcgtcaccga cctgagcggc accatcctgg
    27061 aggtgaaccg ggcctactgc accctcaccg gctacgagcg cgaagacctc atcggccgca
    27121 atccgcgcat cctccaggcc ggcccctccg acgaggcgtt ctacgccgag atgtggggcc
    27181 acctgggccg gcgcggcttc tggcgcggcg acctgcacaa ccgccgcaag gacggcagtt
    27241 actatctgca ggaaaccggc atcaccgcgg tgaccaatgc cctgggcctg cgcacgcact
    27301 acatcggctt cgccgaggac gtcaccctgc tgcgccagag tcagcagcgc ctggaatacc
    27361 tggcctacca cgacgccctc acccagctgc ccaatcgatt gctgctggcc gaccacatcc
    27421 gcctggccct cgccgccgcc cgccgcgaag ggcgtcacgt ggccgtgtgc tacctcgatc
    27481 tggacgagtt caagccggtc aatgaccagt ggggccacac ggtcggcgac cagttgctgg
    27541 tagaggtggc gggacggctg aaggcctgcg tccgcgccga agacacggtg gcccgcctgg
    27601 gcggcgacga gttcgtgatc ctgctggcca gcctggacag caggaccgaa ggcgaacagg
    27661 ccctcgcccg ggtcgtcgcc gccctatcgt cccccttcca gctcaccgtc ggcgagatcg
    27721 gcgtcggtgc gagcatcggc gtgagcgtct tcccgcccga cgacgcagac gccgattccc
    27781 tgatccgcca cgccgaccag gccatgtacc tggccaagca ggccggccgc aactccatcc
    27841 gcttcttcga cccggacgcc gaccggcgcg cccgcagcct gcgggattcg gtggccgagg
    27901 tcgcgctggg cctggagcgg ggcgagttcg agctctacta ccagccccag gtccatctgc
    27961 gcagcggccg ggtggtgggc gtcgaagccc tgctgcgctg gaaccatccc caacgcggac
    28021 tgctggcccc tggcggcttc cttgcagagg tggacaacac cgaagtcgcc gtggccctgg
    28081 gcgagtgggt gctggacacg gccctccgtc aggccgacac gtggcatcgg gccgggctcg
    28141 aactgcgcgt cggcatcaac gtctcggcac atcacctgca gcaggacacc tttgccgcac
    28201 gcctggcgga acgcctctcg cgccaccccg agttgccggc cggccgcatc gagctggaga
    28261 tcctcgagac cgccgccctc gaagacctca gccgcatctc gcggctgatg gaaacctgcc
    28321 gccgcttcgg cgtctccttc gccctcgacg atttcggcac cggctacgcg tccctcgcct
    28381 acctcaagca cctgccggcc aacctgctca agatcgacca gagcttcatc cgcgacatgc
    28441 tgtcggatcc ggaagaccac gccatcgtgc agggcatcgt cggcctcgcc atggcgttcc
    28501 gccgcgaggt catcgccgag ggcgtggaga gtctggccca cggcgaggcg ctcctcgccc
    28561 tcggctgccc cctcgcccag gggtactgca tcgcacgccc catgccggcc gcggccgtcg
    28621 cgccgtgggt tgcccactgg tgcaacccgc cggaatggtc gcagccggcg ccgccagagg
    28681 accggccatg aagcccctgt gcccgcttcc gcctcccgtg gcggggctgg ccccgcccct
    28741 gcgggcctgt gacccctggc tcgtcgtgag cgccgatgcc cgccgcgcgg ccctcagcga
    28801 ggccgcggtc cgactgctgg aactgcccga cgcccacgcc gaattgcccg ccacgataca
    28861 gacggtcctg ggccaggccg acggcgattt cgtcctcagc ctggatggcg ccgacggggc
    28921 acgcttcctc agcgtggcgc tcggcccgga cccggacgcc cgcctcggcg cgggcacccg
    28981 cctcgccgtc ctccacgacg caagcgggct gatccgcctg cagcgcgccc ttgccgacag
    29041 cgaggggcgc ctgcaggcca tcctgggtgc ggcgatggac gccatcatcg ccatggacga
    29101 ggctggccgc atcaccttgt ggaatgccgc ggccgaacgc ctcttcggct ggcgtgccga
    29161 cgaggtgcgg ggccgcgacc tccacgagac cctggccggg acggcggagc gggcccgctt
    29221 cctggcccat gccggacgct ggacacgggg cggcaccagc cctgtgttcg gacagatcct
    29281 gcgcctgccc gcccaccgcc gggacggcac cgccctcgac atcgaactgt ccctcggcgc
    29341 ctttcgccgc catggccact ggcaggccct cggcatcgtc cgcgacgtaa gcgcacgggt
    29401 cgccgccgaa ctggagctgc aggtcaccgg tacccgcctg cagcaggccc tcgaaggcgg
    29461 caacatcacc tattgggact gggacgtgag caccggcagc ctggtcctcg ggccgcgcta
    29521 cttctccatg ctcggctacc cggacgacgc ctttccccac actgcggaga gcttccgcca
    29581 gctcgtccat ccggacgatc ggccgcaggt ggaggcggcg ctgaaggccg tgctgaccgg
    29641 gcagacggcc gagttcgcgt gcgatttccg tctgcgcgcc gccgacggca ccgaccgctg
    29701 ggccaccggc cagggcaagg tcacggcccg cgacgccgcc ggccggccat tgcgggtgat
    29761 cggcacccac cgggacatcc atgcctttca cgagagcgaa gccgccctgc agcgccagct
    29821 cgccgagacc ctgcgcctcc accgggaact ggaggaagcc caggtgcagc tggtgcagag
    29881 cgagaagatg gccgccatcg gccagcttgc cgccggcgtg gcccacgaga tgaacacgcc
    29941 cctgggcttc gtgaaatcca acgtgggcac cctgcagacc tacatctcgc ccctgctgga
    30001 ggtcatcacc gcactgatgg cggccaccga cggcctcccc ggcggggcgg cgctggccga
    30061 cgcccgcgcc cgcgccgccg ccctcgacct ggatttcctg cggcaagacc tgcccgacct
    30121 gttcgcagag acccgcgacg gcatcgtccg ggtccaacgc atcgtcgccg acctcaagga
    30181 cttttcccac gtggacagcg cgacctgggc ccacgccgat ctgcaccggg gcctcgactc
    30241 caccctcaac atcctccaca acgacatcaa gcaccgcatc caggtgatcc gcgactacgg
    30301 cgacctgccc ctgctgtggt gccgcccgtc ccagctcaat caggtcttcc tcaatctcct
    30361 cgccaacgct gcccaggcca tcgccgaccg gggcacgatc accgtgcgga cccgtgccga
    30421 cgcccgccat gtcacggtgg acattgccga caccggctgc ggcatcgccc cggagcacct
    30481 gccacgcatc ttcgacgcct tcttcaccac caaacccgtt ggcaaaggca ccggtctggg
    30541 gctgtcaatc agcggcgaca tcgtgcggaa acacggtggg cacctggaag tgagcagccg
    30601 gcccggcctt ggcagcacct tccgcatcgt cctgcccgtg caggtgccgg atgctccacc
    30661 gcccgagaaa gtctttccgc catgaccgca ccgccccttt ccaccgccgc catgcccccc
    30721 tcgccgacga ccaccgcgac gcacaccctg ctgctggtgg acgacgagcc cgccatcctg
    30781 tcggccctgc gccgcctgtt ccgcctcgcc ggttaccgca tcctcaccgc cgagagcggc
    30841 gccgagggac tcgaattgct ggccgcgcag gcggtggacc tggtcatctc cgacatgcgc
    30901 atgcccgaga tggacggcgc caccttcctc gccgcagtcc gccagcactc gccggagacc
    30961 atccgcatcc tcctcaccgg ctacgccgac ctgtcgtcca cggttgccgc catcaacgaa
    31021 ggcgagatct accgctacgt cgccaaaccc tggaacgacc aggacctgac cctgctcgtg
    31081 catgacgccc tcgagcggcg cgccctgctg cgcgagaacg cccgcctgga cgccgtcacc
    31141 cgcgcccaga acgaagagct gcgggccctc aacgcgggcc tcgaggccaa ggtggcggag
    31201 cgcaccgccg ccctctccgc cgccctcggc cgcgccgacg aggcgcaccg ccagctgcgc
    31261 cagtccttca ccgccatggt gcgggtgttt tccgaactct ccgaggccgg caccgccagc
    31321 gccggtcacg gccgccgcgt cgccgagctg gcgcgcagca tcgcccgcca actgcagctt
    31381 gacgacgcca cccagcagac cgtgatgctc gccggcctgc tccacgacct gggcaaggtc
    31441 ggcctctccg ccgagctcct cgccaagtcc cgcgcccgcc tcagcgccga cgagcgcacc
    31501 gtgctcatgc accacccgaa gcgcgccgaa agcctgctca ccggcatccc gtccctgcgc
    31561 gacgtggccc ggctgatccg ccaccaccac gaaagcttcg acggcagcgg cttcccggac
    31621 cgtctcgtcg gcctggcgat tcccctcggc agccgcatcc tctgcgtcgc caacgattac
    31681 gacggcctgt tgcgcggcac ctcggtggcc ggccgccccc tcagccaccg ggaaaccgtg
    31741 gagttcatgc tggccaaccg gggcaagcgc tacgacccgc ccgtcgtcga ggccctcgtc
    31801 aacgtggtgg cgggcagcga ggcggtggcc gacgatctcg ccgtgaacac ggcccagctg
    31861 cgcgtcggca tggtgctctc cgccgatctg gtgcaccccg aaggctacct gctgctggcc
    31921 cgcgactttc ctctcgatgc cgccatgatc gcccagcttc agcgcctcga gaccagcgaa
    31981 ggcctgcgcc tgcagctgcg tgtgcgccgc ggcgacgacg cgcgatgacc cgcgccgacg
    32041 gcaccctggc gcccttccag tgggcgagct atttcgagac cggctgtgcc gacgtggacg
    32101 cccagcatcg caagctcgtg gacctggtga atgccctggc cgcccgcgcg gcgggcagcg
    32161 gggcactgaa cgccgccgag ctgggccaca tcctcgacgg gctggggcaa tacgcacggc
    32221 accacttcgc caccgaagag gccctgatgg aacgcgccgg tctcgacccg cgccatgtgg
    32281 acgctcaccg cgccgcccac gcggatttcg tcgaacaggt cggagccctg gcaggtgccg
    32341 gcgaatcgga caacgccctg ccgcggctct atgaattcgt ctgcgcctgg ctcacctacc
    32401 acatcctcga caccgaccag gccatggccc gccagctgcg cgcccgcgcc gcaggggtgg
    32461 aggccgccgc cgccttcgac gcggaaggcg ccggcaccgc cgaccccggc aacgccgccc
    32521 tgatcgtggc ggtgcgcagc ctgctgggtg tgattgccgg tcgcaacgcc gccctcgccc
    32581 gggccaatgc cgatctggaa cgccgcgtcg aggcgcgcac ggccgaactc gccaccgccc
    32641 aggcgcgcct gcgggaggcg gacaccctcg ccgcggtcgg ccagctggcc gccggcgtcg
    32701 cccacgagat caacaacccc atcggcttcg tcacctccaa cctcagcacc ctggccacct
    32761 acgccgagca gctgctgacc ctgaccgccg ccgccgacgg actggccgcg cgccatgcca
    32821 cggattccgc ctggcaggcg gcgcgccaga cggtggacgt ggatttcatc cgcgacgatc
    32881 tgtccgccct gctcgacgac gcctcccgcg gtctggaacg ggtgcgcgaa atcgttcagg
    32941 cgctgcaggc gtttgccgcg ccgtcggagg tacgcttcac ccccatcgcg ctggcgccac
    33001 tcctggccga tctgtgccgc agcgtgccga cggccgccgg gcagacggtg cgctgggaaa
    33061 cctgcgacac gccaccgccg gtcgcgggca gcgcgcccca gctccgcgaa gccttccgcg
    33121 ccctgctcga caatggcgtg aaggccctcg caggacagcc gggcacggtg tcggtccgct
    33181 gtaccagcga gaccgcggcc atccgcgtgg acgtggagga tgacggctgc ggcatggcgc
    33241 cgtcggtccg cgcgcacatc ttcgaaccct tcttcaccac ccgcccggtc ggcagcggtc
    33301 gcgggctggg cctttcgtcg gcataccgga tcttccgcca gcacggcggt cgtatcgagg
    33361 tgcgcagcgc gccgggccag ggcagtacct tccaggtgtg gctgccgcgt ctgccagccc
    33421 cggacgccgc ggccccggca gagcatgaaa aagcccggcc ggagccgggc tgaaacgctg
    33481 ctgggggcgc cgcactcagc gcgtcgccag gctgcgcaac cgcgcgaccc gctcctccgt
    33541 ggccgggtgg gtgctgaaca agcccgccag cccgtgcccc gagaggggat tcatgatcat
    33601 catctggccg gtctccgggt gggcctcggc ggtgggcatg gggatgccgc gggcgaaggc
    33661 gtcgatcttg agcagcgcgt cggccagggc gttgggatcg ccgctgatct gcgcgccgcc
    33721 ccggtcggcc tcgaactcac gggcgcggga gatggccatc tggatcaggc tggcggccag
    33781 cggggcgagc agggccacgg cgatggaggc gatggggttg gccgggcggc cttcctcgtc
    33841 acgaccgccg aagaacatgg cgaagttggc cagcgccgag atcgcacccg ccagggtggc
    33901 ggagatcgtg gagatcagga tgtcccggtg ctgcacgtgg gccagctcgt gggccatcac
    33961 gccgcgcagc tcgcgggcgc tgagcatctg caggatgccg gtggtggccg ccacggcggc
    34021 gttgtccggg ttgcggccgg tggcgaaggc gttgggctgg tcttcgtgga tgatgtacac
    34081 gcgcggcatg ggcaggctgg cacgaccggc cagttcgcgg accatgttgt agaggtaggg
    34141 cgaggaggcc tcatccacct cctgcgcgtt gtacatgcgc agcaccatct tgtcggagaa
    34201 ccagtacgag aacacgttca tggcgccgcc gaagacgagc gcgaggagca tgccctgctg
    34261 gccgccgatg aggccgccga cgacaccgaa caaggcgatg atcccggcca tcaggatcga
    34321 ggtcttgagc cagttgccga acattgcggg tatccgtcct ttcgaggatg tggagaaagt
    34381 gacggttaga tgggggaggg tgccgaaaat tcaacggcct gcgtacaggc ccgcgcgggc
    34441 gggccggcac ccgggcaccg cgtcagtcga aacggctgcc cgccgcgatg ggcgcaccgc
    34501 gcaggaaatc cgccgccgac agacgccggc cgccgggctt ctgcagctcg gtgatgcaca
    34561 gggcgccttc gccgcaggcg acgatcacgc cggccccttc ggccagcagc acctcgccgg
    34621 gcgtgccacg ggcgtcgatg gcccgcgccc gccacagctt gaccggcgtg ccctggaagg
    34681 gcgcgaaggc gccggggaag gggttgaagg cgcggatggc gcgctcgatc tcggcggccg
    34741 ggcgggtcca gtccaccacg gcctcggcct tgccgatctt ggcggcgtag gtgacgcctt
    34801 cggccggctg cggcgtggcg gtcagcgtgc cgccgggcag ggcttcgagg gcttcgacga
    34861 tcatctcgct gccgagccag gcaaggcggt catgcaggct ggcggtggtg tcgtcgggct
    34921 ggatggactc ggcgcgcttg agcagcatcg ggccggtatc gaggccggca tccatctgca
    34981 tgatggtgat gccggtctcc gcgtcgcccg cctggagggc ccggtggatg ggcgctgcgc
    35041 cgcgccagcg gggcagcagc gaggcgtgga tgttgaggca gccgtggcgc ggcagatcca
    35101 gcacggcctg gggcaggatg agcccgtagg cggccaccac cagcacgtcg gcggcgcagg
    35161 ccgccagcgg cgcctgctgc tcgggcgtgc gcagcttttc cggctgatgc acggggatgc
    35221 cggcggccag ggccacctgc ttgaccgggc tgggctggag ctgcatgccg cgcccggccg
    35281 ggcgatcggg ctgggtcagg acgaggggca cctcgaaacc ggcggcgagg atggcctcca
    35341 gcgcgatggc ggcgaaatcg ggggttccgg cgaaggcgac tttcatcagg cggtgatccg
    35401 ggccttcttg gccagtttgc tcttgatgcg ggtctgcttg agcagggaca ggtattccac
    35461 gaagac
//
LOCUS       DAOQPA010000023        35402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_497_length_35402_cov_21.783037, whole genome shotgun sequence.
ACCESSION   DAOQPA010000023 DAOQPA010000000
VERSION     DAOQPA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 35402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35402
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_35402_cov_21.783037"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1092
                     /locus_tag="PKX01_05170"
     CDS             406..1092
                     /locus_tag="PKX01_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75547.1"
                     /translation="MKNRYVWIGTCSVLAHLVLFWPEPNRPGDGRRPVISVRLPDYAR
                     GQPPKDETLLMESAETVERAVPMENVAHATPEVPNASGRARTIPAHSVSRGSLVDVSP
                     KAAIATSLQTLGRDGTVAEGGDSDDRPGLLRYRIALASAAVKRVRAELSVVVMQDAVH
                     SLTVEVSGLEAGVTAVRIIASSGRAGLDDMVLRAVELAAGDVPHPFVERGKPWAFTLP
                     VSISQELEAL"
     gene            complement(1139..1687)
                     /locus_tag="PKX01_05175"
     CDS             complement(1139..1687)
                     /locus_tag="PKX01_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ75548.1"
                     /translation="MNFCSQCGSPVTVRVPPGDNLPRHVCDACGTIHYQNPKVVVGAL
                     PVWNEQVLLCRRAIEPRYGLWTLPAGFMENNETAAEGAVRETQEEAGARVELGPMFSF
                     VDIPGISQIHIIFQATLLAPDFSPGEETLEAALFDESDVPWNEIAFRSVEFTLRKFFE
                     DRRSGNYRLHTTALEIHPPLRT"
     gene            1849..3843
                     /locus_tag="PKX01_05180"
     CDS             1849..3843
                     /locus_tag="PKX01_05180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ75549.1"
                     /translation="MTSSFDITKFEYLKASGDLPSPKGVALAVMRALQHDDCSTGELA
                     RIIKTDPAFVGRIVKAANGVAGFNRRAVASVQDAILVLGMRAVRTLALGFSLLSEYRN
                     GVSREFDYPRYWSGCLAQALAMQAIAHHTRAGALEDMFCLGLLARVGELALATLYPAE
                     YDTLQRRRRAEPATAIARLEHELFAISHGELGGAMLADWGLPKVFCVAAEFAEHPERA
                     GHEPGSRSDVLTRSLQMAACMAALFVAPETERAQYLPRLIELGGTLDLPPALVGEISD
                     GVAREWQAWGPLLDVETPDIPSFEVLAAQAADMRAREIEMAAAPDATETIVLHEAQPG
                     ASRPLGVLVVHDDAETRSLIRRVLEVAGHDVREAREGRRALECVLESPPQLLIVDRDL
                     ADGDGLEFVRNLRQTKIGRGLYVLMLMRAGDDDRLIEAFENGADDFVHTPVSPKILAA
                     RLRAAQRVINLQEELERDREEVRRFAAELAVSNRRLQEMALTDSLTGFPNRRYAMERV
                     AQEWSTASRTRRPLSAMVVDVDEFKRFNDNFGHDTGDTILRQVANSLKTALRAQDVVA
                     RTGGDEFLVICPDTHLDAALACAERVRFAVEAGGHASAGGRVTVSIGVATREAGMNDA
                     EALVKRADQALYQAKNKGRNRITTTQTARPAPLVPPRGTH"
     gene            3996..4313
                     /gene="flhD"
                     /locus_tag="PKX01_05185"
     CDS             3996..4313
                     /gene="flhD"
                     /locus_tag="PKX01_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HMZ75550.1"
                     /translation="MRNHALQDEIRELNLAYLMLAQQMLREDRAVALYRLGIGEDVAD
                     VLEGFSAAQLLRMASSTMLLCQFRFNDSMLVDLLASHGRERGTAHLHAAILAAGKPVE
                     ALS"
     gene            4337..4879
                     /gene="flhC"
                     /locus_tag="PKX01_05190"
     CDS             4337..4879
                     /gene="flhC"
                     /locus_tag="PKX01_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HMZ75551.1"
                     /translation="MRNKSVLLEAQDIQKAVELIRLGARMQMLEAETGLSRERLLKLY
                     KEVKGVSPPKGMLPFSTDWFMSWQPNIHASLFMAFHRFVRGNAGLDGLNAIVQAFRLY
                     QEHVESFEMEQVLSLTRAWTLIRFFDARLLQSARCTCCGGEFVTHAYDPTQHYVCGLC
                     HVPSRAGKTRKAQLAESIAP"
     gene            5058..5909
                     /gene="motA"
                     /locus_tag="PKX01_05195"
     CDS             5058..5909
                     /gene="motA"
                     /locus_tag="PKX01_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HMZ75552.1"
                     /translation="MFLIIGYVIILAASLGTYAVHGSLAALWVPTEYIAIVGLTVGGF
                     VAGNGLGAIKGTVAALPTIFKGSPYSKAFYIDVLCMLYEILAKVRKEGLMSIEGDIEN
                     PEGSPILSKYPAFTADHHAVDFLCDYLRMMVGGNLNAFEIENLMDIEMETHHHEAHGP
                     AHVVSKVADSVPAFGIVVAVMGVVNVMGSVGQPPAVLGKMIGGALVGTFLGILISYGF
                     VAPIAGQLEQKVDEGGKIYQCMKAVLLASMNGYAPQVAVEFGRKVLYSTDRPSFAELE
                     EAIKSRK"
     gene            5959..6957
                     /gene="motB"
                     /locus_tag="PKX01_05200"
     CDS             5959..6957
                     /gene="motB"
                     /locus_tag="PKX01_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HMZ75553.1"
                     /translation="MSDDTQQPIVVKKIKKGGAGAHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAQGDLQGIADFFSSPLKVAMAGGSGAGDATSPIVGGGQDLTRSVGQVKRGDV
                     DGKRSMNIEAAKGATRPSGDEGTDAAKKTEQRRERAKLEDLKGQIEAVIEASPSLRPF
                     KNQLLLDITTEGLRIQIVDEQNRPMFDSSSASLKPYTQELLRAIGATLNGVDNTVSLS
                     GHTDSTRYAGGERGFSNWELSANRANASRRELVAGGLAEGKMVRVVGLADTFPLDPAN
                     PAAPINRRISIIVLNKEAEAALRTAGGRKLDVSSESGVDASDIQAGLKPGGRAAPH"
     gene            7108..8316
                     /locus_tag="PKX01_05205"
     CDS             7108..8316
                     /locus_tag="PKX01_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75554.1"
                     /translation="MAQLSPLATVFAGTGWSALVAAGTGFATGDFAAACWAGGLSAVG
                     WGGVLLAGAGRPRDTAAESPRNLDAGQLEPLEAILREASLAIRQQHKGIGAEMHRMQE
                     LLADAIRSLTDSFHGIHAETTAQQSIAVRLTQDNVVDGESSASSFDAFVANTSGVMQR
                     VVDSVIQNSKLGMELVELTDRISRRASDVESILGEITSISKQTNLLALNAAIEAARAG
                     EAGRGFAVVADEVRDLSTRTAQFSQQIGQVMGSMRESVKCTEEAIEKMASTDMTFALE
                     SKQQVEHVLSAMEGINEQRGEAIVRIGEHAQAMDGLVNRAVTALQFQDLVSQLVSRIE
                     QRVRVLDEVLMEVEGLAARIDQSAGTGNPAILHDAVGLLRARLDAMQAASAPASADHP
                     EVSRGEIDLF"
     gene            8339..8704
                     /locus_tag="PKX01_05210"
     CDS             8339..8704
                     /locus_tag="PKX01_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75555.1"
                     /translation="MAKTVLTVDDSASIRQMVSFTLKSAGYEVVEAVDGMDGLDKAKT
                     RSCNLVLTDQNMPRMDGLSLIKTLRGMPQYRSVPILMLTTESSDTMKSQGRAAGATGW
                     LVKPFDPQKLIEVVKKVIG"
     gene            8742..11042
                     /locus_tag="PKX01_05215"
     CDS             8742..11042
                     /locus_tag="PKX01_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ75556.1"
                     /translation="MAIDMSQFYQVFFEEAAEHLATMVALLLAIDPGAPDPEEVNALF
                     RAAHSIKGSSGTFGFQDMMEVTHILETMLDKVRKGEAPLTDDMVDASLVAGDVLKNLL
                     AAHRGEEEADREAAEAICHRLEALCRALEQGGDAAAPAPLAVPVQTGTGAVGPACFNL
                     HFTLACPPEAMATQLEQLRVELRGLGQVETLRAPDATGDECHLRIQTDLPSDNLRSIL
                     EFVADAASVRVEPQALDAGAEAYGFFAPHDEPVAPATAEPYGFFDPLEPSAPAPQPEP
                     YGLFEDIVPQAAGAQESEAYGFFTELPAPADPIDVAPPSVPSLQADGKEAYGFFEPEP
                     LRVAEPAPESMAAPESAPRPVAVPAAVKKGPPARVGASGDSGSIRVSVEKVDQMINLV
                     GELVITQAMLTQAASNVDPVIFERLINGLAQLERNTRDLQESVMSIRMMPMSVVFSRF
                     PRVVRDLSQKLGKQVELKTIGENTEMDKSLVERITDPLTHLVRNSLDHGLETPDKRRA
                     AGKPETGTITLSAYHQSGNIVIEVGDDGAGLNRARILAKARERGMPVSDAMSDQDVWA
                     LIMEPGFSTAEQVTEVSGRGVGMDVVKKNISAMGGRIELDSMPGVGTRITVRLPLTLA
                     ILDGMSVGVGPETYIIPLGYVMESLQPERGMMKSVSGLERLIQVRGEYLPVVCLHEVF
                     RIHGAITDWSKGIMVVLEADGQSAALFIDQLIGQHQVVIKSLEANYRRVPGIAGATIM
                     GDGHVALILDVPMLVNMARRLMPVAA"
     gene            11179..11682
                     /locus_tag="PKX01_05220"
     CDS             11179..11682
                     /locus_tag="PKX01_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ75557.1"
                     /translation="MLQAAHTAAAGEAEETGFTQELLTFTLGAEEYAIDILKVQEIRG
                     YDTVTSIANAPAFIKGVINLRGTIVPIVDLRIKFSVGKVEYTPFTVVIILNLNGRVVG
                     IVVDAVSDVIALKPEQMRPAPDFAASVDTAYIMGLGALGDRMLIVMDIEKLMLSGEMA
                     LVDEAAH"
     gene            11764..14862
                     /locus_tag="PKX01_05225"
     CDS             11764..14862
                     /locus_tag="PKX01_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75558.1"
                     /translation="MNRLSVQGKMLLLAVIGLIPIVVLAFMLLARIDADLSFTTKETY
                     TVPMVKPARQLMQAVQTHRAVARQVITGQADAARLEESRARIAEAIRAEDDFQSQHGA
                     MLETDKAWAALRAAWDELKAQSTRVGASESIQLHNAYIARIRDFISHAADQTNATLDP
                     ELETYYLMDIFISRLPELIEDVGSIRALGADVIPRGRTTERERIELNIVVRALRTGID
                     TVNSEVGKAGKEDATVHALLDTPAATFAATVQDYATLVERELLRAEGVTVTYGNVFDS
                     AAKAIDAGYVFFDLSAQDFDRRLAVRMAKLEQARSVAIAVIAVSLGVALYLFLGFRHT
                     MLGAIAEIRNGVARMAGGTLDQNLAVVGQDAFAEIGGAINGMQASLLAKIEADQMTAA
                     SNLRIRNALDKASTNIMLADNDGQIIYCNDSVLKMLRGAESDLRKDLPNFSADGLVGR
                     NFDSFHRNPAHQRNLLSRLSGEHRASIQVGGRTFSLVANPVLNNEGVRLGTVVEWVDR
                     TSEVLVEAELSGLLNAAVSGDFAQRLSMEGKEGFFATLAAGMNQLMGIVATNLEDVAR
                     VLNAIARGDLTEKITADYGGTFGQLKDDTNTTVERLREVVTQIKEASEAINVAAQEIA
                     AGNSDLSGRTEEQASSLEETASSMEQLNATVRQNAENARQAKDLASSSNAVAEQGGAM
                     VGRVVETMGAIQESAKKISDIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVAS
                     EVRSLAQRSAQAAREIKGLIADSVNQVEDGARLVNEAGHTMEDVVGNFQKLSALVTEI
                     AEASAEQSGGIEQVTQAVGQMDEVTQQNAALVEEAAAAAESLEDQARTLVRAVTMFRL
                     AAGAPAAPASLDFDGAIQAHMQWKHKLRNFLAGEGDPLDADVVSRDDKCVLGCWIHGE
                     GQRFGHMSSYTRLKHKHAAFHACAGSVIRAKQAGDEAAANALLMGDFAVLSDETIREI
                     RDLRAVCGSQAATPAAAAPAARAPSNVSPLPVRNLKSGAPLPKVKQSAPSNLAENEWE
                     EF"
     gene            14913..18152
                     /locus_tag="PKX01_05230"
     CDS             14913..18152
                     /locus_tag="PKX01_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HMZ75559.1"
                     /translation="MKINMPVTQREIELGDAEVIVSKTDLKGQITYVNREFTAISGFS
                     EDELIGKSHNIVRHPDMPPEAFEDMWRDLKAGRPWVGIVKNRCKNGDHYWVEAHATPV
                     RENGAITGYMSVRRKPTRAQIEAAGHAYALFRDGKAGGRAICHGQVVSAGLQRRLVHG
                     FANLSVAAKAAVATALCLSLILGGGFLLLRERTTAMLESQAMSVVTDKVAAVKALVEV
                     AAASAKRETDRLNDVFVAGFPEGFVAHEGKDGVPVLSSGAVVLNQRTLEVDRFSGASG
                     AAVTVFVRKGDEFIRVASSLKQENGERLTGSTLDHDNPAYAKLVSGQRYTGRAQLSGR
                     DIYASYAPLKDAAGKVIGGVFVGRRFAEELEALKAQVKAVRFGETGYAYVLDGTPGKS
                     QGTLMVHPNKEGQNLLDAKDTTGRSIVREMLERKQGAMTYPWVNAEQNEQVVRNKVVA
                     FDSFPDWNWVIVGGTNVEEFESLAAAVSRQEAVIGTTATIALCVLVYFLLRRFVAAPL
                     RLSVAAAQTVASGRYDYPMDVTRNDEIGQLLQALESMQVKLGNDIAEQKRIGDENARI
                     RQALDSVETNVRIADDSGRVIYANMALMKTVRAIEAEIRQHAPGFRADTFVGSSIGLL
                     YPDPQAALGRLAALQQTVRTQMVIGGRNFEVVTSPIFNAAGTRLGSVGEWRDQTDELA
                     AEKEIQAIVAAAGDGDFSARLDIDGKTGFFRDLSGGINRLMEGVSASLNDLARVLNAI
                     ARGDLTEKITADYGGTFGQLKDDTNTTVERLREVVTQIKEASEAINVAAQEIAAGNSD
                     LSGRTEEQASSLEETASSMEQLNATVRQNAENARQAKDLASSSNAVAEQGGAMVGRVV
                     ETMGAIQESAKKISDIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSL
                     AQRSAQAAREIKGLIADSVNQVEDGARLVNEAGHTMEDVVGNFQKLSALVTEIAEASA
                     EQSGGIEQVTQAVGQMDEVTQQNAALVEEAAAAAESLEDQARTLVRAVTMFRLSEGAQ
                     ATPRLEAPPENVAVLPERSARPVVARASAPLPKVRRAVGGSTDEEWEEF"
     gene            18170..19090
                     /locus_tag="PKX01_05235"
     CDS             18170..19090
                     /locus_tag="PKX01_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HMZ75560.1"
                     /translation="MTVQPPVRRSGAVPAPSRDARPAPTGTSSLQARDREFDFSPADF
                     ELVRSLIYKHAGITLSPIKQDMVYSRLARRLRARGDRTFAAYLERAQREPEEWETFVN
                     SLTTNLTSFFREAHHFDILAERLKKGGSRTPVKIWCSAASTGEEPYSLAITACEAFNT
                     MTPPVQIVASDIDTHVLATAQKGVYGIERLERLSRERVQRFFLRGTGTQEGYARVRPE
                     LQRLVEFKRINLLDAGWPLQGPFDYLFCRNVMIYFDKPTQHAILKRFAPLLKAEGLLF
                     AGHSESFLHAADVFRSLGRTVYERADRRAS"
     gene            19062..19691
                     /gene="cheD"
                     /locus_tag="PKX01_05240"
     CDS             19062..19691
                     /gene="cheD"
                     /locus_tag="PKX01_05240"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HMZ75561.1"
                     /translation="MKGLTAVHPDPGYIEHLSTNRYFDRTFGCDAVKVLPGEYFVTTS
                     DIVLVTVLGSCVTACVRDREKGVGGMNHFMLPEGGAEGMLSTSARYGAYAMEVLLNHL
                     LKMGARRTNLEAKVFGGGRVMASLQQSQVGEKNAAFVLDYLATERIPVVAEDLLDIYP
                     RKVYFFPASGRVMMKKLVSVRNNTVLEREREYEARLRRDNLQGDVELFG"
     gene            19729..20823
                     /locus_tag="PKX01_05245"
     CDS             19729..20823
                     /locus_tag="PKX01_05245"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMZ75562.1"
                     /translation="MAIKVLVVDDSALMRSVLTEIINSAPDLEVVGGAPDPLVAREMI
                     KTLNPDVLTLDVEMPKMDGLDFLARLMRLRPMPVVMISSLTQRGSETTLQALELGAVD
                     FVPKPKLDSLGGIESCRVEICEKIRGAYGSRPRVPVAFIAPSKPLPGEKSSGFAGRSG
                     GSEGGAGLDKLLCIGASTGGTEAIKEVLCSLPEKMPGILMVQHMPEMFTASFAKRLDG
                     LCSLNVKEAEHGEKVLPGTAYLAPGHSHLSVRRVSGGYVCELSRSEPVNRHRPAVDVL
                     FHSAAKQAGANALGVILTGMGKDGAQGLLAMRQAGAWTLGQDQDSCVVYGMPREAAQI
                     GALEEVAALKDIGARVLARLRGGERRGMRA"
     gene            20883..21188
                     /locus_tag="PKX01_05250"
     CDS             20883..21188
                     /locus_tag="PKX01_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ75563.1"
                     /translation="MDTNVSTQQSQARIQLSGRFDFNSHREFRDAVDKALGSPGTTEV
                     AVDLGNVAYLDSSALGMLLMLRDKAKTSGKTVCLANAKGSVRQVLDIANFGKLFPLV"
     gene            21188..22927
                     /locus_tag="PKX01_05255"
     CDS             21188..22927
                     /locus_tag="PKX01_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HMZ75564.1"
                     /translation="MSSEQSPESLFILVVDDNPDDLYLTRRDLEALGHRVALASTARE
                     ALALIEEAYPDVLMTDVGLPGMDGIELLQTVRARADGSWIPSLVFTGSLDEATQMRAL
                     EAGADAYLCKPASRKMLRAKLGVILRLRAMQRQAEARNKELAHYRVLQEEEQRLARFV
                     MERLINREALGDPALHHWIHSAAEFSGDLIAAARTPGNVLHVLLADGAGHGLAASLNV
                     LPVTAPFYRMTARGFGIDAIAREINNKVRALLPVDRFIAATLVAVNFRERYVTVWNGG
                     NPGPLLLRPDGAVHMHFNRHHLPLGVADEDEFDATVDAHVVDPRDHLLLFSDGLIELE
                     DASGRPLGMEGLGDALAGVLARDRLPRLTEIVHRMIGGKPPPDDISVAQIALHDEPHA
                     LPSAGSGEPTGPIVASDWRLVLRLGATDLRRLDAVPMLLGAIDHFDSVRPFAGRLFLV
                     LSELFNNALDHGLLRLPSSLKAGADGMDLYLEERARRLAELTAGAVEFELVLRQTPSL
                     SPALHITCADSGPGFNHEELVEAARGEPSDEDLLKSHGRGIPLLLKVCEDVRYRGPGN
                     RVEVVVRLSERAG"
     gene            23142..24821
                     /locus_tag="PKX01_05260"
     CDS             23142..24821
                     /locus_tag="PKX01_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75565.1"
                     /translation="MNIQLIAGLAGIGSAAGAAGIGVAFGVPLAPGFAAACSALGLAL
                     GGATYGLLRAVCVTPLRSLRTTIEDMHRDGDLSRRASVPHGYAEDLARVFNELIESFQ
                     GIVGKVIFDAQRVAEAAGTLSGQAGAVAQGSEEQRGAAESMVGAIEQMTAGVNAVAGH
                     ASQTASNAQVARDLSVQGVEIVTQASREIERIAQSVADSARVIGALGERSQAISGIVK
                     VIREIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTSTATTEIGGMIDAI
                     QAETRSAISAIDAGSKQAHAGADLARRAAESLRQINEGARETMERIDEIAEAITSHSH
                     DADQIVGNVRQIMLMVDRNSGEARETLDEAGSLNGLATNLKEISRVFKLGPAGEQAMQ
                     AHAKMPDMVRSVAAQIGELLEQAVSSGQVQVDDLFDENYRAIANTRPQKYNTRFDALT
                     DRLFPTIQEALLAKHPEMVYAIGTDRNGYVPTHNKRFSQPLTGDYNRDFTGNRSKRIF
                     DDPVGKQCGRHEMPFLIQTYRRDTGEIMHDISAPVYVQGRHWGGFRIGYKA"
     gene            25003..25971
                     /locus_tag="PKX01_05265"
     CDS             25003..25971
                     /locus_tag="PKX01_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HMZ75566.1"
                     /translation="MSELLKNIDARTRLAGTNKLEILLFTLGVDARTGRRETFGINVF
                     KVREVMRTPPITAAPEMPAAVEGMVSLRGVLVPVVDLAKYAGMGADSAREIMIVTEYN
                     GHTQGFLVEAVDTILRLDWSQMRVPPDMMTANMGGLVTAVTELPGPRLVMMLDVEKVL
                     SETTRYDDDLMFKGIEPIKCEEPVTVYFADDSSVARKQIERTLDTLGVRYVGSVNGRA
                     AWDELQKLAHYAESAGRKVKDLLQMVLTDVEMPEMDGYILTKKIKSDPRFEGVPVVMH
                     SSLSGMSNQQLGRSVGVDEYVPKFEPQRLAEVLRRLVDRCQSARAA"
     gene            25987..26940
                     /locus_tag="PKX01_05270"
     CDS             25987..26940
                     /locus_tag="PKX01_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HMZ75567.1"
                     /translation="MDTVLAERGLLEAVDARTKLAGSNRMEILLFSLGTQEIFGINVF
                     KVREVSRTPFITKAPNMPDGVEGLISLRGNVIPVLALAKIMGIAAPGEPLGGSMMVTE
                     YSKRTLGFLVHEVDRIIRVEWDKVRAPDNVTTSGNHFITAITELADGKLVSILDVETI
                     LASTFGEAVVGQIHPLEGGHDVNVFFVDDSAVARKKIGETLDKLGVKHKHALNGLEAW
                     TRLEGMASHAQQRGVCLADELSLILVDAEMPEMDGYVLTRNVKSDARFEGIPVVMHSS
                     LSSEANRAMGKSVGVDSYVAKFDADILAETLRPLLLRAQRD"
     gene            26956..27354
                     /gene="cheY"
                     /locus_tag="PKX01_05275"
     CDS             26956..27354
                     /gene="cheY"
                     /locus_tag="PKX01_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HMZ75568.1"
                     /translation="MSDPKMKFLVVDDFSTMRRIVRNLLKELGFTNVDEAEDGQIALQ
                     KLNSLPFDFVVTDWNMPNMDGLTLLQNIRATPSLKHLPVLMITAEAKKENIIAAAQAG
                     ASGYIVKPFTAATLSEKLEKIFEKMGKKAA"
     gene            27385..28221
                     /gene="cheZ"
                     /locus_tag="PKX01_05280"
     CDS             27385..28221
                     /gene="cheZ"
                     /locus_tag="PKX01_05280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HMZ75569.1"
                     /translation="MSKRLKFDESGDSDELQALFDSMAGVAAPASPPPAPPKLEVVAP
                     ADKAGDDDDLQALFDSVSSAYTPPAVEGIASEHSCDVVFNRIGTMTRRLHDTLRELGY
                     DRSLQEAAHAIPDARQRLSYIAEMTEQAASKVLNATDIAKPIQDTVESGATALRARWD
                     QLYANQLSPDEFKALAGETRSFLGDLVQHSRATNAQLLEIMMAQDFQDLTGQVIKKVV
                     ELAQGLESQLLRVLIEAMPAERKAEAPEGLMNGPVISAVGRDDVVTSQEQVDDLLESL
                     GF"
     gene            28244..30184
                     /locus_tag="PKX01_05285"
     CDS             28244..30184
                     /locus_tag="PKX01_05285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMZ75570.1"
                     /translation="MSDFSGMEDLLQDFLVEAGDLLSGVDNKLVDLERAPDDRGLLND
                     IFRGFHTIKGGAGFLNASELVTLCHLTENLFDKLRNGELAVTPETMDVILASTGAVRD
                     MFGYLEQGVQPAAADPQLIEDLRLAIAGALRPAGVVTSAPEVLPAVVAAPPTPVTPAP
                     GGADGPDWNALFDAVTSKAPATATAVVPAADPPPVHQSAEDLAAKSPEQVIKAAVGRR
                     ATDKPGASGPIGRREGERQRDNSIRVDTARLDQVLNLSGEIGLTKNRLTALRSDILNG
                     RADTETLHALDLAVSQLDLLVSDLQNAVMKTRMQPIGRLFQKYPRIARDLARNLGKDV
                     ELVLSGEETEIDKTMIEDLSDPIIHLIRNAVDHGVEETSERRANGKPEKSIVRLEARQ
                     EGDHIVILVADDGRGMNAERLRAKALEKGLISDEEANTMDERQSYNLVFLPGFSMASK
                     VSDVSGRGVGMDVVRTNIQKLNGSIDIRSSLGKGTTFIISLPLTLAILPVLLVRLGDQ
                     PFAVPLSMVREILPIEPAEVQEVGGRATMVVRGEVLPIMPLATLLGWDQVQTPEYGVL
                     MQSAEQSFILAIDSFAGREDAVIKSLDDFRPKGVAGVTTLSNGQIVLILDMKELLGAL
                     GDTRGISRRALLAHLPVPALAA"
     gene            30346..30687
                     /locus_tag="PKX01_05290"
     CDS             30346..30687
                     /locus_tag="PKX01_05290"
                     /inference="COORDINATES: protein motif:HMM:NF022777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HMZ75571.1"
                     /translation="MGGFEFDDWRFLAENDPAAFFRARERTLRQFITQHPDQRDALGA
                     LQMRIDTARAMSGTPQQACRDLFGLLEEHLLLLSERLADLRREATTLQRMLGVDGAEL
                     AVESPSVRGHS"
     gene            30740..31891
                     /gene="flhB"
                     /locus_tag="PKX01_05295"
     CDS             30740..31891
                     /gene="flhB"
                     /locus_tag="PKX01_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HMZ75572.1"
                     /translation="MADDSDLEKTESASARRLEQAREEGQVPHSRELATFIALMAGVT
                     ALYVLGDWAGQRMLHLFRSGLTLERSRAFAPDAMGQIFEQLALDALLTLAPLFVVLIV
                     AALVTPFLMGGWVFTTKVLTFDVSRLDPLQGLGRMFSTHGLAELVKASLKSVIVASVG
                     VWVVWRERDHLFALMLQPVEESVGEFASLLLLSSLLIVVGLGIIAAMDVPYQLWEYHR
                     KLRMTKEELRQEMKEQEGDPQIKARIRAAQREMSRRRMMSQVPKADVVVTNPTHYSVA
                     LHYDPDKAGAPVVVAKGVDLVALNIREVAREHGIPILEAPPLARALYAHCELEDPIPA
                     TLYTVVAEVMAYIFQLNHWIAEGGLPPEVPTGLTVPEGLDPAGTGEVSA"
     gene            32804..34894
                     /gene="flhA"
                     /locus_tag="PKX01_05300"
     CDS             32804..34894
                     /gene="flhA"
                     /locus_tag="PKX01_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HMZ75573.1"
                     /translation="MNDSLNLRALFAPANIRQLAGPLLVVLILAMMVLPLPAFVLDLM
                     FTFNIAVSVMVMLVAMYTRKPLDFSVFPTVLLVTTLLRLSLNVASTRVVLLEGHTGPD
                     AAGKVIEAFGHFLVGGNTAVGLVVFIILTVINFVVITKGAGRIAEVGARFTLDAMPGK
                     QMAIDADLNAGLIDEKEAKRRRAETAQEADFYGAMDGASKFVRGDAVAGILIMGINVV
                     GGLIVGVIQHNMELGQAANNYTLLTIGDGLVAQLPALIISVAAGLVVSRVGDEGDMGS
                     QVVGQVFSNPQVLVLTAAILGVLGLIPGMPNFVFLLLAGGLGALAWYGGKRQAAIAEE
                     EVPAAAAPAVPPEAQEASWTDVTPVDVLGLEVGYRLIPMVDKAQDGELLKRIRGLRKK
                     FARDIGFLSSAVHIRDNLELKPNAYRIVLKGVEVGNGMAFPGQFMAINPGRVSGPLNG
                     RETTDPAFGLPAVWVDAGQREQAHALGYTVVDASTVVATHLNHLILSHAAELLGRTET
                     QALLDHIAKDNPKLVEDLVPKLLPLATVQRVLQNLLDEGVNIRDMRSIVEVLAECAPR
                     VQDPVELTTRVRQALGRAIVQGLFPGNGEIQVMALDPGIERVLLQAIGGGGPEGGAIE
                     PGLADTLLRETAVAAQRMEDIGLPPVLLVPPALRWLLSRFLRRSVPTLKVVSNAEVPE
                     TRLIRVASMITVAR"
     gene            35042..>35402
                     /gene="flhF"
                     /locus_tag="PKX01_05305"
     CDS             35042..>35402
                     /gene="flhF"
                     /locus_tag="PKX01_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264594.1"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HMZ75574.1"
                     /translation="MNVKRYFAKTAREALRQLKEELGPDAVVLSNRAVNGGVEILALP
                     ANDVAALQRARQQAAAAAPAAPVMPAAASTEVPRAAARASQPAADFFDDDFRLSLSRA
                     AAARTQPAAAPAAAGWVR"
BASE COUNT         6225 a        11262 c        11834 g         6081 t
ORIGIN      
        1 cagttcgccc gccaagcggg ccgccgcgta gtcaccgcgc atccgctcaa gcatcctgtc
       61 tagcacgcag aacgtgaaaa gcgacagcaa tcctatgaac gatgcgacag tggatgtatt
      121 aatcctccac ctttcgatcg acaattcgga taatgatgtc aatcttcagg ttgtcaggca
      181 tttccgggca tctcgccact tttttgagtg cgctcgccgc cacgtagcgt tggaacatct
      241 gagagcccct tcttccgaaa tgtcgtgacg ctacatcaac attgccgtgg caatgtccgt
      301 gaattctgat gtttaaagcg cccgattgtt gttatggatg tcctggggtc gggccgcaca
      361 gcatctggtt gttggtggtg tgctcagtct gtcggtgccg agatcatgaa aaatcgctat
      421 gtctggatag ggacctgttc agtgttggcg cacttggtct tgttctggcc ggaacccaat
      481 aggccggggg atggccgacg gccggtcatt tcggtgcgat tgcctgacta tgcccggggt
      541 cagccgccca aggacgaaac gctccttatg gagtcggcgg agactgttga acgggctgtt
      601 ccgatggaga acgtcgcgca tgcgacccct gaggtgccaa acgcctcagg tcgagccagg
      661 acgattccgg cacatagcgt gtcccgcggg tctctggtag atgtatcgcc gaaggcagcc
      721 atcgccacgt ctttgcagac gcttgggcgt gacgggaccg tcgcagaagg gggcgattca
      781 gatgacaggc ccggtctgct gcgttaccgg atcgcactgg cttctgctgc agtgaagcgt
      841 gtgcgagccg agttgtctgt ggtggtgatg caggacgcgg tgcattcgct taccgtcgaa
      901 gtcagcggat tggaggccgg cgtgacggcg gttcgcatca tcgcgtcgtc tggacgggcg
      961 ggcctggatg acatggtctt gcgcgcggtc gaattggcgg ccggagatgt tccgcacccg
     1021 tttgtcgaac gggggaaacc atgggcgttc acgctgcctg tcagtatttc gcaggagttg
     1081 gaagccttgt gaagccatgc ccactgatct tgggcgtacg gggctaccgg agcggcattc
     1141 aggtgcgtaa cggtggatgg atctcgagcg ccgtggtgtg cagccgatag tttcctgatc
     1201 tccgatcttc gaaaaacttc cggagcgtga actccaccga tcggaacgca atttcgttcc
     1261 agggcacgtc cgattcgtcg aacagagccg cttccagggt ttcttcacca ggactgaagt
     1321 ccggagccag tagcgtggcc tgaaagatga tgtggatctg gctgataccc ggaatatcca
     1381 caaagctgaa catggggccc agttctacgc gggcgccagc ttcttcttga gtctcccgca
     1441 ccgcgccctc ggcggcggtt tcgttgtttt ccatgaatcc ggccggaagt gtccagagcc
     1501 cgtagcgtgg ttcgatggcg cggcggcaca gcagcacctg ctcattccac accggcaggg
     1561 cgccgaccac gaccttgggg ttctggtaat ggatcgtccc gcacgcgtca cagacgtggc
     1621 gtggaaggtt gtcgccgggg gggacacgca ccgtcaccgg cgagccgcat tgggagcaga
     1681 agttcatgaa caagcgtgga tcatggggag ccagtcggca ttctagtcga aggtccgtgt
     1741 gctttacacg gccggcggat ggggcacgat cgacttaagg ggatgaggtt tctgtccgat
     1801 aagtcgtgga ctacggccga gtgccgcgtc atcccgtgca aggcaacgat gacttcttcc
     1861 tttgacatta cgaagttcga atatctcaag gcgtcgggcg atctcccttc gccgaagggg
     1921 gttgcactgg cggtcatgcg cgccctgcag cacgacgatt gttcgactgg cgaactggcc
     1981 cgcatcatca agaccgatcc cgcgttcgtc ggacgcatcg tcaaggcggc caatggcgtg
     2041 gcgggcttca atcgtcgggc cgtggcctcg gtgcaggatg cgatcctggt gctcggcatg
     2101 cgggctgtcc gtacactggc cctggggttc tcgctgctgt cagagtaccg caacggtgtg
     2161 tcgcgggagt tcgactatcc gcgctactgg tcgggctgtc tggcacaggc tctggcgatg
     2221 caggccatcg cgcatcacac gcgtgcgggt gcccttgaag acatgttctg tctcggactg
     2281 ctggcgaggg taggcgaatt ggcgctggca accctgtacc cggctgaata cgacacgttg
     2341 cagcgacgcc ggcgcgccga acccgccacg gccatcgcga ggctcgagca tgaattgttt
     2401 gccatctccc acggggaact ggggggcgcc atgctggcgg attggggtct gcccaaggtc
     2461 ttctgcgtgg ccgcggagtt tgctgaacat cctgaacgtg ccgggcatga gccgggatcc
     2521 cgttcggatg tcctgacccg ctcactgcag atggctgcat gcatggctgc gctgtttgtc
     2581 gctccggaga cggagcgggc ccagtatctg ccgcgattga tcgaactggg gggcaccctc
     2641 gatctgccgc cggcgcttgt cggtgagatc agtgacggcg tcgcgcggga gtggcaggcg
     2701 tgggggcccc tgctcgatgt ggagacaccc gacattcctt cattcgaggt gcttgcggcc
     2761 caagcggcgg acatgcgggc gcgcgagatc gagatggccg cggcccctga tgcgaccgag
     2821 acgatcgtgc tgcacgaagc gcagcccgga gcgtcccgcc cgctcggcgt gttggttgtt
     2881 cacgacgatg ccgagacgcg cagtctgatc cggcgggtgc tggaggttgc cgggcatgat
     2941 gttcgagagg ctcgggaagg gcgtcgggcc ctcgagtgcg tgctcgaaag cccgccccag
     3001 ttgttgatcg tggaccgtga tctcgcggat ggggacggac tggagtttgt gcgcaacttg
     3061 cgccagacga agatcgggcg cggattgtat gtgctgatgc tgatgcgtgc cggggatgat
     3121 gaccggctga tagaggcctt cgagaacggt gcggacgact ttgtccacac gccggtcagc
     3181 cccaagatcc tcgctgcacg tctgcgtgcc gcgcagcggg tgatcaatct tcaggaggaa
     3241 ctggagcggg atcgggagga ggtgcgccga ttcgccgcgg agctggcggt ctcgaaccgg
     3301 cgcctccagg aaatggccct gacggattcc ctgaccggct ttccgaaccg ccgctatgcc
     3361 atggagcggg ttgcccagga atggtcgacg gcatcccgca cccgccgtcc cttgtcagcg
     3421 atggtcgttg acgtggatga gttcaagcgc ttcaacgaca actttggcca cgataccggc
     3481 gatacgatcc tgcgtcaggt ggctaattcc ctcaagacgg ccttgcgtgc ccaggatgtg
     3541 gtggcacgga cggggggcga cgagtttctg gtgatctgtc ccgacacgca tctggatgcg
     3601 gccctggcct gtgcagagcg cgtgcgcttt gccgtggagg cgggcggtca tgccagcgca
     3661 ggcggcaggg tgacggtgag catcggtgtg gccactcggg aagcgggaat gaatgatgcc
     3721 gaggcgctcg tcaaacgcgc cgaccaagcc ctttatcagg ccaagaacaa ggggcgcaat
     3781 cgcatcacca ccacccagac cgcacgtccg gcgcccctcg ttccgccccg cgggacccat
     3841 tgaaccgggt acgtttgctt acatctgtag gacaaattct tacacagtcg cagcccgtgg
     3901 gcttacgcga atattcgaag ccgacggatg ttgaatcttg ccggtgaacg gcagaatttg
     3961 ccgcatgaga gtccttattc ttcaaggagt gaatcatgcg caatcacgca ctgcaggatg
     4021 agatccggga actgaatctc gcctatctga tgctcgccca gcagatgctg cgggaggacc
     4081 gggctgtcgc cctctaccgc ctcggcatcg gtgaggacgt ggcggacgtg ctcgaaggtt
     4141 tcagcgcggc ccagctgctg cgcatggcca gttcgaccat gttgctgtgc cagtttcgtt
     4201 tcaacgacag catgctggta gacctcctgg cgtcccatgg tcgggaacgt ggcacggcgc
     4261 acctgcatgc agccatcctc gctgccggca agcctgtcga agccttgtcc tgaagtgcgc
     4321 gcaggaggga cggatcatgc gtaacaagag cgttctgctg gaagcccagg acatccagaa
     4381 ggcggtcgaa ttgatccggc tcggtgcacg catgcagatg ctggaggcgg aaaccggatt
     4441 gagccgcgag cgtctgctca agttgtacaa ggaagtgaag ggggtgtcgc ccccgaaggg
     4501 catgctgccg ttttccacgg actggttcat gtcctggcaa ccgaacatcc acgcctcgtt
     4561 gttcatggcc tttcaccggt tcgtccgtgg caatgccggc ctggacggac tcaacgccat
     4621 cgtccaggcc tttcgtcttt accaggaaca cgtcgaatcc ttcgagatgg agcaggtgct
     4681 gagcctcacc cgcgcctgga cgctgatccg cttctttgat gcccgcctgc tgcagtccgc
     4741 ccgctgcacc tgttgtggcg gcgagttcgt cacgcacgcc tacgatccga cccagcacta
     4801 tgtgtgcggc ctctgtcacg tcccgtcccg cgccggtaag acccgcaagg cgcagcttgc
     4861 cgaatcgatt gctccctgac cctcctcttg cctgagaggt tgtgccgctc gacgctcgcg
     4921 agcggcattt ttttgcggac tgtcctgcgg gacgggtgct caagttcgtt caggcagggc
     4981 cgttattccg accgatggat taacgatctg cgggccgcgc atggaattgc gccggccgcc
     5041 cctgagaggt tctggcgatg tttctgatca tcggttacgt catcattctg gcagcttcgc
     5101 tgggcaccta cgcggtgcac ggcagcctcg cggccctgtg ggtgccgacc gagtacatcg
     5161 cgatcgttgg tctgacggtc ggcgggttcg tggccggcaa cggcctgggt gcgatcaagg
     5221 gcacggtggc ggccctgccg acgatcttca agggttcgcc ctattccaaa gcgttctaca
     5281 tcgacgtcct gtgcatgctc tacgagatcc tcgccaaggt gcgtaaggaa ggcctgatgt
     5341 cgattgaagg ggacatcgaa aatcccgagg ggagcccgat cctgtccaaa tatccggcct
     5401 tcacggcgga tcaccatgcc gtggatttcc tctgcgacta cctgcgcatg atggtgggcg
     5461 gcaatctgaa cgccttcgag atcgagaacc tgatggacat cgaaatggaa acccaccacc
     5521 acgaagccca cgggccggcc cacgtggtgt cgaaagtcgc cgactccgtg ccggccttcg
     5581 gcatcgtcgt ggcggtgatg ggcgtggtca acgtaatggg ctcggtgggc cagccgcccg
     5641 ccgtgctggg taagatgatc ggcggtgcgc tggtggggac tttcctgggg atcctcatct
     5701 cttacggctt cgtcgcaccg atcgccgggc agctcgaaca gaaggtggac gagggcggca
     5761 agatctatca gtgcatgaag gcggtcttgc tggcgagcat gaacggctac gccccgcagg
     5821 tggcggtgga gtttggacgc aaggtgctgt attccaccga tcgccccagc tttgcagagc
     5881 tggaagaggc catcaagagc aggaagtaag cggcatgcgg cggggcaata aacccggcga
     5941 tgcggcggaa tccaggccat gagcgacgat acccaacagc ccattgtcgt caaaaagatc
     6001 aagaagggcg gcgccggtgc ccacggcggt gcctggaaga tcgcctatgc cgacttcgtg
     6061 acggcgatga tggccttctt cctgctgatg tggctgctgg gctccacggc ccagggcgat
     6121 ctgcagggca ttgcggattt cttcagttcg cccctcaagg tggccatggc gggtggctcc
     6181 ggggccggtg acgcgaccag tcccatcgtc ggcggtggcc aggatctgac ccggtcggtg
     6241 gggcaggtga agcgcggcga cgtggacgga aagcgttcca tgaacatcga ggctgccaag
     6301 ggcgcaaccc gcccctccgg cgatgaaggc acggatgcag ccaagaagac cgagcagcgc
     6361 cgcgagcggg ccaagctgga ggacctgaaa ggccagatcg aggccgtcat cgaagccagt
     6421 ccgagcctgc gccccttcaa gaaccaactg ctgcttgaca tcaccaccga aggcctgcgc
     6481 atccagatcg tcgatgaaca gaaccgaccc atgttcgatt cgtccagtgc ctccctcaag
     6541 ccctacaccc aggagctgct gcgcgccatc ggcgccaccc tcaacggcgt ggacaacacc
     6601 gtcagcctgt ccggccatac ggattccacg cggtatgccg gcggcgagcg cggcttttcc
     6661 aattgggagc tgtccgccaa ccgggccaat gcgtcccggc gagaactggt cgccggcggg
     6721 ctggccgaag gcaagatggt gcgggtggtg ggccttgcgg acacctttcc cctcgacccg
     6781 gcgaatcctg ctgcgcccat caaccgacgc atcagcatca tcgtgctcaa caaggaagcc
     6841 gaggctgcct tgcgtaccgc aggcgggcgc aagctcgacg tctccagcga gagcggcgtc
     6901 gatgcgtccg atatccaggc cggcctgaag cccggcgggc gggcggcgcc ccactgacgg
     6961 ttccgtcacg ccgggggccg cgcagcaggc tggggcgtgc atcgccagtc gctgcgggcc
     7021 ttcctggcgt cccgcggaag tgtccgaagc taaagatgcc ggtccgcgtg acgatatgtt
     7081 caccaggaag ttttgctcga attcatcatg gcccagctct ctcccctcgc taccgtgttt
     7141 gccggaaccg gctggagcgc cctcgtcgcg gccggaaccg gctttgcaac cggtgatttt
     7201 gctgccgcct gctgggcagg tggtctcagc gccgtcgggt gggggggcgt gctgctcgct
     7261 ggggccgggc gcccccgcga cactgcggcg gaatcgcccc gcaacctgga tgcgggccag
     7321 ctggagccgc tggaggcgat cctccgcgag gccagcctgg cgattcgaca gcagcacaaa
     7381 ggaatcggtg ccgaaatgca ccggatgcag gaactcctgg ccgatgcgat ccggtcgctg
     7441 actgacagtt tccacggcat ccatgccgag acaactgcgc agcagagcat tgccgtgcgt
     7501 ctcacccagg acaacgtcgt ggacggcgag agcagcgcga gcagcttcga cgccttcgtg
     7561 gccaatacgt caggcgtgat gcagcgggtc gtggacagcg tgatccagaa cagcaagctt
     7621 gggatggaac tcgttgaact gacggaccgg atctccaggc gcgcgagcga tgtggagtcg
     7681 atcctcggcg agatcacgtc catttccaaa cagaccaatc tcctcgccct caacgccgcc
     7741 atcgaggcgg cccgcgccgg cgaggccggt cgaggctttg ccgtggtggc cgacgaggtg
     7801 cgtgatctgt ccacccgcac cgcccagttc agccagcaga tcgggcaggt gatgggctcc
     7861 atgcgcgaaa gcgtgaagtg caccgaggaa gccatcgaga agatggcgtc caccgacatg
     7921 acctttgccc tcgaatccaa gcagcaggtg gagcatgtgc ttagcgcgat ggaggggatc
     7981 aacgagcagc gcggcgaggc gatcgtgcgc atcggcgagc atgcccaggc catggatggc
     8041 ctggtcaatc gcgcggtgac cgcgctccag tttcaggatc tggtgtccca gctggtcagc
     8101 cgtatcgagc agcgcgtacg ggtgctggat gaggttttga tggaggtcga ggggctggcg
     8161 gcccgcatcg accagtcggc cggcacaggc aacccggcca ttttgcacga cgcagtcgga
     8221 ttgctgcgcg cgcgcctcga cgccatgcag gcggcgtccg cgcccgcgtc ggcggatcac
     8281 ccggaggtct cgcgcggcga gattgatttg ttttagcttc aacccctagg aaacagcgat
     8341 ggccaagacc gtactcaccg ttgatgactc tgcctccatc cggcagatgg tctccttcac
     8401 cctgaaaagt gccggctatg aggtcgtcga agcggtcgac ggcatggacg gcctcgacaa
     8461 ggccaagacc cgcagctgca atctggtgct gaccgaccag aacatgccgc gcatggacgg
     8521 cctgtccctc atcaagaccc tgcgcggcat gccccagtac cgttcggtac cgatcctgat
     8581 gctcaccacc gagtcgtccg acaccatgaa gtcccaggga cgtgcggccg gtgccacggg
     8641 ctggctggtc aagcctttcg atccgcagaa gctcatcgaa gtcgtcaaga aagtgatcgg
     8701 ctgagccggt cacgcgacgt cgtcgagtaa gggagacagg catggccatc gacatgagcc
     8761 agttctatca ggtgttcttc gaggaggccg ccgagcatct ggccaccatg gtggccctgc
     8821 tgctggcaat cgatccgggc gcacccgatc cggaggaggt gaacgccctg ttccgggcgg
     8881 cgcattccat caaggggtcg agcggaacct tcggcttcca ggacatgatg gaggtcaccc
     8941 acatcctgga aaccatgctc gacaaggtgc gcaagggcga agcgcccctc accgacgaca
     9001 tggtggatgc gtccctggtg gcaggcgacg tgctcaagaa cctgctcgca gcccatcggg
     9061 gtgaggaaga ggccgaccgg gaggccgcgg aagccatctg tcaccgcctg gaggccctgt
     9121 gccgagcgct cgagcagggt ggggacgccg cggcccctgc gccgctcgcc gtacccgtgc
     9181 agacgggcac cggggccgtg ggaccagcct gtttcaatct ccacttcacc ctggcctgcc
     9241 cgcccgaggc aatggcgacg caactggagc agctgcgggt cgaactgcgc ggactcggcc
     9301 aggtcgagac cttgcgcgcg ccagatgcta ccggtgacga atgtcacctg cgcatccaga
     9361 ccgatctgcc ctcggacaat ctgcgcagca tccttgagtt cgtcgcagat gcagcgtccg
     9421 tgcgcgtcga gccccaggcg ctcgacgcgg gcgcggaggc ttacggattc tttgcgccgc
     9481 acgacgagcc cgtggcgccg gccaccgcgg aaccctacgg gttctttgat ccgctcgaac
     9541 cctcggcccc ggcgccgcaa ccggaaccct acgggttgtt cgaggacatc gttccgcagg
     9601 ccgctggcgc ccaggaaagc gaagcgtacg gcttcttcac ggaactgcct gcgccggccg
     9661 accccatcga tgtggcgccg ccctcagtgc cttcgctgca ggccgacggc aaggaagcgt
     9721 acggattctt cgagcccgag cccctgcggg tcgccgagcc ggccccggag tccatggctg
     9781 cgcccgaatc cgcaccgcgt ccggtggccg ttccggccgc cgtcaaaaag ggtccgcctg
     9841 cgcgggtcgg ggccagtggt gacagcggtt cgatccgcgt cagcgtcgaa aaggtggatc
     9901 agatgatcaa cctggtgggc gaactggtga tcacccaggc catgctcacc caggccgcca
     9961 gcaatgtgga cccggtcatc ttcgagcgcc tcatcaatgg cctcgcccag ctcgagcgca
    10021 acacgcggga tctgcaggaa tcggtcatgt cgatccgcat gatgcccatg tccgtggtat
    10081 tcagccgctt cccgcgggtg gtgcgggacc tgtcccagaa gctgggcaag caggtggagc
    10141 tgaagaccat cggcgagaac accgagatgg acaagagcct ggtggagcgc atcaccgatc
    10201 cgctgaccca cctggtgcgc aacagcctcg accacggcct ggaaacgccc gacaagcggc
    10261 gcgcagccgg caagccggag accggcacga tcaccctgtc ggcgtatcac cagagcggca
    10321 atatcgtcat cgaggtcggg gacgacggcg ccggactcaa ccgcgcccgc atcctcgcca
    10381 aggcccgcga acgggggatg ccggtcagcg acgccatgag cgaccaggac gtctgggcgc
    10441 tgatcatgga gccggggttc tccactgcag agcaggtgac cgaagtgtcc ggccgcggtg
    10501 tggggatgga cgtggtgaag aagaacattt ccgcaatggg cgggcggatc gagctggatt
    10561 ccatgcccgg cgtggggacg cgcatcacgg tgcgcctgcc tctgaccctg gccattctgg
    10621 acggcatgag cgtgggcgtg ggccccgaga cctacatcat cccgctgggc tacgtgatgg
    10681 aatcgctcca gcccgagcgc ggcatgatga agagcgtctc cggcctcgag cggctgatcc
    10741 aggtgcgcgg cgaatacctg ccggtggtgt gtctccacga agtgttccgc atccatggtg
    10801 cgatcactga ttggtcgaaa ggcatcatgg tggtgctgga ggcggacgga cagagcgccg
    10861 cgctgttcat cgaccagctc atcggtcagc accaggtggt catcaagagc ctggaggcca
    10921 attatcgtcg cgtgcccggc attgccggcg ccaccatcat gggcgacgga cacgtggccc
    10981 tgatcctcga cgtgccgatg ctggtgaaca tggcccgacg gctcatgccg gtggcggcct
    11041 gagcgcggac ccgagccgcg tgcacgtccc caaaagccgc ctgcgggcgg cttttgcacg
    11101 tctgccggca ggattcccga tgcttcagaa agcgtggtgc ctgccgtaaa agaagcatcc
    11161 ccttcgagga gtcatcccat gcttcaggct gcccacaccg ccgctgccgg cgaggcggaa
    11221 gaaaccggct ttacccagga actgctcacc ttcaccctcg gtgccgagga gtacgccatc
    11281 gacatcctca aggtgcagga gatccgcggc tacgacacgg tgacctcgat cgccaacgcg
    11341 ccggcgttca tcaagggtgt catcaatctg cgcggcacca tcgtgccgat cgtcgatctg
    11401 cggatcaagt tttccgtggg caaggtggaa tacacgccgt tcacggtggt gatcatcctc
    11461 aacctcaacg gccgggtggt gggcatcgta gtggatgccg tctcggacgt gatcgcgctc
    11521 aaaccggaac agatgcggcc ggcgcctgat ttcgcggcgt cggtggatac cgcctacatc
    11581 atggggctgg gtgccctggg cgatcgcatg ctgatcgtga tggacatcga gaagctgatg
    11641 ttgtccggtg agatggccct ggtggatgaa gcggcccact gagcggcgtc gcccccgaag
    11701 caaccccttc cggattcagg acgaacaaac atggctgcct ttctctctcc cgctgtcggg
    11761 gtcatgaacc gactttccgt gcagggcaag atgctcctgc tggccgtcat cggcctcatc
    11821 cccatcgtcg tgctggcctt catgttgctg gcgcgcatcg acgccgatct cagcttcacc
    11881 accaaggaaa cctacacggt gcccatggtc aagccggccc gccagctgat gcaggcggta
    11941 cagactcatc gggccgtcgc ccgccaggtg atcacgggtc aggccgatgc tgcgcgtctg
    12001 gaggagtccc gcgcgcggat cgccgaggcc atccgcgccg aagacgactt tcagagccag
    12061 cacggggcga tgctcgagac ggacaaggcg tgggcagccc tgcgtgccgc ctgggacgaa
    12121 ctgaaagccc agtccacccg tgtcggggcc agcgaaagca tccagctgca caacgcctac
    12181 atcgcgcgta tccgtgattt catctcccac gcggcggatc agaccaatgc cacgctcgat
    12241 cccgagctgg aaacctatta cctgatggac atcttcatca gccgtctgcc cgagctgatc
    12301 gaggatgtgg gcagcatccg tgcactgggg gccgatgtta ttccccgcgg gcgcaccacg
    12361 gagcgtgagc gcatcgagct gaacatcgtg gtgcgcgcac tccgcacagg gatcgatacg
    12421 gtgaacagcg aggtgggcaa ggccggcaag gaggatgcga ccgtccatgc cctgctcgac
    12481 acgcctgcgg ccacctttgc ggcgaccgtc caggactacg ccaccctggt ggagcgtgaa
    12541 ctgctccgag ccgaaggtgt gaccgtcacc tacggaaatg ttttcgattc cgcagccaag
    12601 gccatcgacg ccggctacgt gttctttgat ctgtccgcgc aggatttcga ccgccgcctg
    12661 gccgtccgga tggccaagct ggagcaggcc cggagcgtgg cgatcgcggt gatcgcggtg
    12721 agcctgggcg tggccctcta cctgttcctc gggttccgcc acaccatgct gggcgccatt
    12781 gccgagatcc gcaacggggt cgcacgcatg gcgggcggca cactcgatca gaatctcgcg
    12841 gtggtcgggc aggatgcatt cgcggagatc ggtggcgcaa tcaatggcat gcaggccagc
    12901 ctcctggcca agatcgaggc tgaccagatg acggctgcca gcaacctgcg catccgcaac
    12961 gccctcgaca aggcgtcaac caacatcatg ctggccgaca acgacggaca gatcatctac
    13021 tgcaacgact cggtcctgaa aatgctgcgg ggcgcggaga gcgatctgcg caaggatctg
    13081 cccaacttca gcgccgacgg actggtcggg cgcaacttcg acagcttcca ccggaatcct
    13141 gcccaccagc gcaacctcct gtcgcgcctg agcggcgagc accgtgcttc catccaggtc
    13201 ggcgggcgaa ccttctcctt ggtcgccaat ccggtgttga acaacgaagg tgtgcgcctg
    13261 ggcaccgtgg tcgaatgggt ggaccggacg tccgaggtgc tcgtggaggc cgagctttcg
    13321 gggctgctca atgccgcggt ctcaggggat ttcgcccagc gcttgtccat ggaaggcaag
    13381 gagggcttct ttgcgacgct cgcggcgggc atgaatcagc tgatggggat cgtggccacc
    13441 aatctggagg acgtggcccg cgtgctcaac gcgatagcgc ggggcgacct gacggagaag
    13501 atcacggcgg actacggcgg caccttcggc cagctgaagg acgacaccaa caccacggtg
    13561 gagcggctgc gggaggtggt gacgcagatc aaggaggcct cggaggcgat caacgtggcg
    13621 gcgcaggaga tcgccgcggg caactcggat ctgtcgggcc gcaccgaaga gcaggcgagc
    13681 agcctggaag agaccgcgag ctcgatggag cagctcaacg ccacggtccg ccagaacgcc
    13741 gagaacgccc gccaggccaa ggatctggcc agctccagca acgcggtggc cgagcagggc
    13801 ggcgcgatgg tcgggcgggt ggtcgagacc atgggcgcga tccaggaaag cgccaagaag
    13861 atctcggaca tcatcggggt gatcgactcc atcgccttcc agaccaacat cctggccctc
    13921 aacgccgcgg tggaagcggc gcgggcgggc gaacagggtc gtggcttcgc ggtggtggcc
    13981 agcgaagtgc gcagtctcgc ccagcgcagc gcccaggcgg cgcgggagat caaggggctg
    14041 atcgccgaca gcgtgaatca ggtcgaagac ggcgcccggc tggtgaacga ggcgggccac
    14101 accatggaag acgtggtggg caacttccag aagctgtcgg cgctggtgac cgagatcgcc
    14161 gaggcgagtg cggaacagag cggcggcatc gaacaggtga cccaggcggt cggccagatg
    14221 gacgaagtga cgcagcagaa cgcggcgctg gtcgaagagg cggcagcggc ggcggagagt
    14281 ctggaggatc aggcccgcac gctggtgcgg gcggtcacca tgttccgcct cgctgccgga
    14341 gcccccgcgg cgcctgcgtc gctggatttc gacggggcga tccaggccca catgcagtgg
    14401 aagcacaagc tgcgcaattt cctggcaggc gaaggcgatc cgctcgatgc ggacgtggtc
    14461 tcgcgggacg acaaatgcgt gcttggatgc tggatccacg gtgaagggca gcgttttggt
    14521 cacatgtcgt cctacacgcg gctcaagcac aagcatgcgg ccttccatgc ctgcgccggt
    14581 tcggtgatcc gcgccaagca ggccggtgac gaggccgccg ccaacgccct gctgatgggg
    14641 gatttcgctg tgctgtccga cgagacgatc cgtgagatcc gcgacctgcg cgccgtatgt
    14701 ggttcgcagg ccgccacgcc cgctgccgcg gcacccgctg cccgtgcgcc cagcaatgtc
    14761 tcgcccctgc cggtgcgcaa cctcaagtcc ggagcgccgc tgccgaaagt caagcagagc
    14821 gcaccttcca atcttgccga aaacgagtgg gaagagttct gacgccttga tgccaatccc
    14881 tgtcgtcgcg acggggccac agggagtacc cgatgaagat caacatgccg gtcacccagc
    14941 gggagatcga actaggcgac gccgaggtca tcgtctccaa gacggacctg aagggccaga
    15001 ttacctacgt caaccgtgaa ttcaccgcga tcagcggctt ttccgaggat gaactgatcg
    15061 gcaagtccca taacatcgtc cgtcatccgg acatgccgcc ggaagccttc gaggacatgt
    15121 ggcgcgacct caaggcgggc cggccgtggg tagggattgt caagaaccgg tgcaagaacg
    15181 gcgaccatta ctgggtcgag gcccatgcca cgccggtccg ggagaacgga gcgatcaccg
    15241 ggtacatgtc ggtccggcgc aagccgaccc gcgctcagat cgaggccgcc gggcatgcct
    15301 atgccctgtt ccgcgacggc aaggccggcg ggagggcgat ctgtcacggc caggtagtgt
    15361 ccgccgggct gcaacgccgt ctggtgcacg gctttgccaa tctttccgtc gcggccaagg
    15421 ccgccgtggc gacggccctg tgtctctccc tgatcctcgg cggcggcttc ctgctgctgc
    15481 gcgagcggac gacggcgatg ctggagtccc aggcgatgtc cgtggtgacc gacaaggtgg
    15541 ccgcggtgaa ggcgctggtc gaggtggctg ccgcgtccgc caagcgggag acggaccggc
    15601 tcaacgacgt gttcgtggcg ggctttcccg agggctttgt ggcacacgag ggcaaggacg
    15661 gggttcctgt gctcagcagc ggtgcggtgg tgctcaatca gcgcacgctg gaagtcgatc
    15721 gcttcagcgg ggcgagcggc gccgccgtga cggtgttcgt gcgcaagggc gatgagttca
    15781 tccgggtggc ctcgtccctc aagcaggaga acggcgaacg tctgaccggc tccaccctcg
    15841 atcacgacaa tccggcctac gcaaagcttg tgtccggtca gcgctacacc ggacgcgccc
    15901 agctgtccgg acgggacatc tacgcgagct acgcgcccct caaggatgcg gccggcaagg
    15961 tgatcggcgg cgtgttcgtc gggcgtcgct ttgccgaaga gctcgaggcc ctaaaggccc
    16021 aggtcaaggc ggtccgcttc ggtgaaacgg gctacgccta cgtgctggat ggcacccctg
    16081 gcaagtcgca ggggaccttg atggtgcatc cgaacaagga agggcagaac ctgctggacg
    16141 ccaaggacac caccgggcgt tcgattgtgc gggaaatgct ggagcgcaag cagggtgcga
    16201 tgacgtaccc ctgggtgaac gccgagcaga acgaacaggt cgtgcggaac aaggtcgtgg
    16261 ccttcgacag ctttccggac tggaactggg tgatcgtggg cggcaccaac gtcgaggagt
    16321 ttgaaagtct ggccgcggcc gtaagcaggc aggaggccgt catcggcacg accgccacca
    16381 ttgcgttgtg cgtgctggtg tacttcctgc tgcgccgctt cgtggccgcg cccctgcggc
    16441 tcagcgtggc cgccgcccag accgtggcga gcgggcgcta cgactatccc atggatgtga
    16501 cccgcaacga cgagatcggt cagctgttgc aggcgctgga gtccatgcag gtcaagctcg
    16561 gcaacgacat cgccgaacag aagcgcatcg gcgacgagaa tgcgcgcatc cgtcaggcgc
    16621 tcgacagtgt tgaaacgaat gtacggatcg ccgacgacag cggccgggtg atctacgcca
    16681 acatggcgct gatgaagacc gtgcgcgcca tcgaggccga gatccgccag catgcgccgg
    16741 gcttccgggc ggacaccttc gtcggcagca gcatcggcct gctctatccc gaccctcagg
    16801 ctgccctggg acggctggca gcgttgcagc agaccgtgcg gacccagatg gtgatcggcg
    16861 ggcgtaattt cgaagtggtg accagcccga tcttcaatgc ggccgggacc cgcctcgggt
    16921 ccgtgggcga atggcgcgac cagaccgacg aactggcggc ggagaaggag atccaggcca
    16981 tcgtggcggc ggcgggcgat ggcgatttct ccgcgcgtct ggacatcgac ggcaagacag
    17041 gcttcttccg tgacctgtcg ggcggcatca accgcctgat ggaaggcgtc tcggccagtc
    17101 tcaacgacct ggcccgcgtg ctcaacgcga tagcgcgggg cgacctgacg gagaagatca
    17161 cggcggacta cggcggcacc ttcggccagc tgaaggacga caccaacacc acggtggagc
    17221 ggctgcggga ggtggtgacg cagatcaagg aggcctcgga ggcgatcaac gtggcggcgc
    17281 aggagatcgc cgcgggcaac tcggatctgt cgggccgcac cgaagagcag gcgagcagcc
    17341 tggaagagac cgcgagctcg atggagcagc tcaacgccac ggtccgccag aacgccgaga
    17401 acgcccgcca ggccaaggat ctggccagct ccagcaacgc ggtggccgag cagggcggcg
    17461 cgatggtcgg gcgggtggtc gagaccatgg gcgcgatcca ggaaagcgcc aagaagatct
    17521 cggacatcat cggggtgatc gactccatcg ccttccagac caacatcctg gccctcaacg
    17581 ccgcggtgga agcggcgcgg gcgggcgaac agggtcgtgg cttcgcggtg gtggccagcg
    17641 aagtgcgcag tctcgcccag cgcagcgccc aggcggcgcg ggagatcaag gggctgatcg
    17701 ccgacagcgt gaatcaggtc gaagacggcg cccggctggt gaacgaggcg ggccacacca
    17761 tggaagacgt ggtgggcaac ttccagaagc tgtcggcgct ggtgaccgag atcgccgagg
    17821 cgagtgcgga acagagcggc ggcatcgaac aggtgaccca ggcggtcggc cagatggacg
    17881 aagtgacgca gcagaacgcg gcgctggtcg aagaggcggc agcggcggcg gagagtctgg
    17941 aggatcaggc ccgcacgctg gtgcgggcgg tcaccatgtt ccggctcagc gaaggcgctc
    18001 aggcgacgcc acgactcgag gcgccgccag aaaacgtggc ggtgctcccg gagcggtccg
    18061 ccagaccggt agtggcccgc gccagtgcgc ccctgcccaa ggtgcgtcgg gccgtgggcg
    18121 gctcgaccga cgaggaatgg gaagaatttt gagctgagcg gatgctgcca tgactgtcca
    18181 accacccgtg cggcgttccg gtgccgtgcc cgcgccgtcg cgggatgccc gcccggcgcc
    18241 gaccggcacc tcctcactgc aggcccgcga ccgggagttc gatttctcgc cggccgactt
    18301 cgagctggtg cgttcactga tctacaagca cgccggcatc accctgtcgc cgatcaagca
    18361 ggacatggtg tacagccgac ttgcccggcg tctgagggcg cgtggtgacc ggaccttcgc
    18421 ggcctacctg gagcgcgccc agcgcgagcc cgaggagtgg gaaaccttcg tcaacagcct
    18481 gacgacaaat ctcacctcct ttttccggga ggcccatcac ttcgacatcc tggctgagcg
    18541 cctgaagaag ggtggcagtc gcacaccggt gaagatctgg tgcagtgcgg cctccaccgg
    18601 cgaagagcct tattccctgg cgatcaccgc ctgcgaggcc ttcaacacga tgactccgcc
    18661 ggtgcagatc gtcgcctcgg atatcgacac ccacgtgctg gccaccgccc agaagggcgt
    18721 ctatggcatc gagcgtctcg agcgtttgtc gcgggagcgc gtgcagcgct ttttcctgcg
    18781 cgggaccgga acccaggaag gctacgcgcg ggtacgtccc gagttgcagc gcctggtgga
    18841 attcaagcgc atcaacctgc tggatgcggg ctggcccttg caggggccat tcgattacct
    18901 gttctgccgc aacgtgatga tctatttcga caagcccacc cagcacgcca tcctgaagcg
    18961 tttcgcgccg ctcctgaagg cggagggcct gctctttgcc ggacattccg aaagcttcct
    19021 gcacgcggcc gatgtgttcc gctcattggg gcggaccgtc tatgaaaggg ctgaccgccg
    19081 tgcatcctga tccggggtat atcgagcatc tgtccaccaa ccgctatttc gaccggacct
    19141 tcgggtgcga tgcggtgaag gtgctgccgg gagaatattt cgtcaccacg tcggacatcg
    19201 tcctggtgac ggtgctgggc tcttgcgtga cggcctgtgt gcgcgaccgg gagaagggcg
    19261 tggggggcat gaatcatttc atgctgcccg aaggtggcgc ggagggcatg ttgtccacgt
    19321 cggcacgcta tggcgcatat gccatggagg tgctgctcaa tcacctgctc aagatggggg
    19381 cacgccggac gaacctggag gccaaggtgt tcggaggcgg cagggtcatg gccagcctgc
    19441 agcagtccca ggtgggcgag aagaatgcag ccttcgtcct tgactatctg gccaccgagc
    19501 ggattccagt ggtggccgag gatctgctcg acatctatcc gcgcaaggtc tatttctttc
    19561 cggccagcgg ccgggtgatg atgaaaaagc tggtgtccgt gagaaacaac acggtgctcg
    19621 aacgtgaacg cgaatacgaa gcgcgtctca gacgcgacaa tttgcagggc gacgtcgagc
    19681 tatttggatg accggacatg ccgtccggga actgactgga agcgatccat ggcaatcaag
    19741 gtgctcgtcg tcgatgattc cgcgttgatg cggagcgtgc tgacggaaat catcaattcg
    19801 gctcccgatc tggaagtggt gggtggagca cccgatcctc tggtggcccg ggaaatgatc
    19861 aagacgctca acccggacgt gctgaccctc gacgtggaaa tgcccaagat ggacgggctc
    19921 gattttctgg cccgtctgat gcggttgcgg ccgatgccgg tggtgatgat ttcgagcctg
    19981 acccagcgcg gatcggaaac caccttacag gcgctagaac tcggtgcggt ggatttcgtg
    20041 cccaagccca aactcgattc gttgggaggt attgaatcct gtcgtgtaga aatttgtgaa
    20101 aagatcaggg gcgcatatgg ttcaaggccg cgtgtgccag tggcatttat agcaccctcc
    20161 aagcccttgc ccggagagaa aagcagcgga tttgccggtc gttccggcgg gtccgaaggt
    20221 ggggccggtc tggataaact gctctgtatc ggtgcgtcga ccgggggcac cgaggcgatc
    20281 aaggaagtgc tatgttctct tccggagaaa atgcccggaa tcctgatggt gcagcacatg
    20341 ccggagatgt tcaccgcatc gttcgcaaag cggcttgacg gcctgtgcag cctgaacgtc
    20401 aaggaggccg agcatgggga aaaggtgttg ccgggcacgg cctatctcgc cccggggcat
    20461 tcgcatcttt cggtgcggcg tgtgtcgggc ggttacgtgt gtgaattgtc ccgatcggaa
    20521 ccggtcaatc gtcacaggcc cgccgtagat gtgttgttcc attcggcggc caagcaggcg
    20581 ggggccaatg ccctgggcgt gattctgacg ggcatgggca aggatggcgc gcagggtttg
    20641 cttgccatgc ggcaggcggg ggcgtggacc cttggacagg atcaggattc gtgtgtcgtg
    20701 tatggcatgc cccgggaggc ggcgcagatc ggtgccctcg aggaagtggc ggcgctgaag
    20761 gacatcggcg cgcgggtgct ggcccgtctc cgcgggggag agcgtcgcgg gatgcgcgcg
    20821 tgaagaatga aggttgccgg gtggccggca cggttgtacg taacagttaa tagaggcatt
    20881 acatggacac caatgtcagc acccagcaga gccaggcgag gattcagctc tccgggcgtt
    20941 tcgatttcaa ttcccatcgg gaattccgcg atgcggtgga caaggcgctg ggcagtcccg
    21001 ggacgaccga ggtcgccgtg gacctgggta atgtggccta tctcgacagt tccgcccttg
    21061 gcatgctgct gatgcttcgt gacaaggcca agacgtccgg caagaccgtc tgtctggcca
    21121 acgccaaggg cagcgtgcgt caggttctcg acatcgccaa ttttggcaag ctctttccgt
    21181 tggtttgatg tcttcggagc aaagccccga atccctcttc atcctggtcg tcgatgacaa
    21241 tccggacgac ctctacctga cccgccgcga tctggaggcc ctgggccacc gggtcgcgct
    21301 cgcgtccacc gctcgtgaag cgctcgccct catcgaggag gcctacccgg acgtcctcat
    21361 gacggacgtc ggcctgcccg gcatggacgg catcgaactg ctccagacag tgcgtgcgcg
    21421 ggcggacggt agctggattc ccagtctggt ctttacgggc agtctcgacg aggcgacgca
    21481 gatgcgtgcc ctcgaggccg gcgccgacgc ctatctgtgc aagccggcct cgcgcaagat
    21541 gctgcgcgcc aagctggggg tgatcctgcg cctgcgggcc atgcaacggc aggccgaggc
    21601 gcgcaacaag gaacttgccc actaccgcgt cctccaggaa gaggagcagc gtctggcgcg
    21661 cttcgtcatg gagcgcctca tcaaccgcga ggccctcggc gatccggccc tgcatcactg
    21721 gatccattcg gcggccgagt tcagtggtga ccttattgcc gcggcccgta cgccgggcaa
    21781 cgtgctgcac gtcctgctgg ccgacggtgc cggtcacgga ctggctgcat ccctcaatgt
    21841 gctgcctgtc acggcaccgt tctaccggat gacggcccgg ggcttcggca tcgatgccat
    21901 tgcccgcgag atcaacaaca aggtgcgcgc cctgctgccg gtggatcgct tcattgcggc
    21961 aaccctggtg gcggtcaact tccgcgaacg ctacgttacc gtgtggaacg gcggcaatcc
    22021 cgggccgttg ctgttgcgcc cggacggcgc cgtgcatatg cacttcaacc gccatcacct
    22081 gccccttggg gtggcggacg aggatgagtt cgacgctacc gtggacgccc atgtggtgga
    22141 tccccgtgac cacctgctgc tcttttccga cggcctgatc gaactggagg atgcatccgg
    22201 acgcccgctc ggcatggaag gtctgggcga tgcgctggcc ggcgtgctgg ccagggatcg
    22261 cctgccgcgc ctgaccgaga tcgtccatcg catgatcggc ggcaagcccc cgcccgacga
    22321 catctccgtc gcccagatcg cgctccacga cgagcctcac gccctgccga gcgcgggctc
    22381 gggggagccc accggcccga tcgtggcgag tgactggcgg ctggtcctgc gcctgggtgc
    22441 cacggatctg cgccggctcg atgcggtgcc gatgcttctc ggcgccatcg atcacttcga
    22501 cagtgtccgg ccgttcgccg gccgcctgtt cctggtgctg tcagaactct tcaacaacgc
    22561 cctcgatcat ggcctgctgc gccttccgtc gagcctcaag gcgggcgccg acgggatgga
    22621 tctgtacctg gaggagcggg cccggcgact ggccgaattg acggcgggcg ccgtggagtt
    22681 cgagctggtc ttgcgtcaaa cacccagtct gtcgcccgcc ctgcacatca cctgtgcgga
    22741 ttcagggcct ggcttcaacc acgaggaact ggtcgaggcg gcccgtggcg agccgtccga
    22801 cgaggatctg ctcaagagcc atgggcgggg tatccccctc ctgctcaagg tctgcgagga
    22861 cgtccgctac cgggggcccg gcaatcgggt cgaggtggtg gtccgcctgt ctgagcgcgc
    22921 cggctgaaat tttttccggc tccgcccggc ggatactgtg gttttccaca aattgcggcg
    22981 ttttttttaa gtttttgtgt agctgaccga taaggggcca aaccggcccc gccttggaga
    23041 cgtgtctcag cgggtcgcgt ccggtgccgt gtgggccggg tgggagccct tccgatggaa
    23101 ggcgctcgat tcaagaccat tgacgaggtc cgtcctccat catgaacatt caactcattg
    23161 caggactcgc cggaatcgga agcgcagcag gcgcggcggg catcggtgtg gccttcggcg
    23221 ttccgctcgc gccggggttt gcggcggcat gcagcgcgct tgggttggcc ctgggtggcg
    23281 ccacctacgg cctgctgagg gcggtctgcg tcacgccgct tcggtccctg cggacgacca
    23341 tcgaggacat gcaccgggac ggcgacctgt cgcgccgggc gtccgtgccc catggctacg
    23401 ccgaggacct ggcacgggtc ttcaacgaac tgatcgaaag ttttcagggc atcgtcggca
    23461 aggtcatctt cgacgcccaa cgggtcgccg aggcggccgg caccctgtcc ggccaggccg
    23521 gtgccgtagc ccagggctcc gaggagcagc gtggcgctgc cgaatccatg gtgggcgcca
    23581 tcgagcagat gactgccggc gtcaacgcgg tggccggcca cgccagccag accgcgtcga
    23641 acgcccaggt ggcccgggat ctgtcggttc agggggtgga gatcgtcacc caggcgtccc
    23701 gcgagatcga acgcatcgcc cagtcggtgg ccgattccgc gcgggtcatc ggcgctctgg
    23761 gggaacgctc ccaggccatc agcggcatcg tcaaggtgat ccgcgagatc gcagatcaga
    23821 ccaatctgct ggcgcttaac gcagccatcg aggcggcgcg ggcaggcgag caggggcgcg
    23881 gttttgccgt cgtggcggac gaggtgcgca agctggcaga acggacgtcc accgccacca
    23941 ccgagatcgg cggcatgatc gacgccatcc aggccgaaac ccgcagcgcc atttcggcca
    24001 tcgatgcggg cagcaagcag gcgcacgccg gggctgacct ggcccgccgg gcggccgagt
    24061 ccctgcgcca gatcaacgag ggcgcccgcg agacgatgga acgcatcgac gagatcgccg
    24121 aggccatcac ctcccacagc cacgatgcag atcagatcgt cggcaacgtg cggcagatca
    24181 tgctgatggt ggaccgcaac tccggcgagg ctcgggagac cctcgacgag gccggtagcc
    24241 tcaacggact ggccaccaac ctgaaggaaa tcagccgggt cttcaagctt ggtccggcag
    24301 gcgagcaggc catgcaggcc cacgccaaga tgccggacat ggtccgcagc gtggctgcgc
    24361 agatcggaga gctcctggag caggccgtgt cgtccgggca ggtccaggtc gacgacctgt
    24421 tcgacgagaa ctaccgggcc atcgccaaca cccggccgca gaagtacaac acccgcttcg
    24481 atgccctcac cgaccgcctc ttcccgacga tacaggaggc gcttctggcc aagcacccag
    24541 agatggtcta cgccatcggc accgaccgca atggttacgt accgacccac aacaagcgct
    24601 tctcccagcc cctgaccggc gattacaacc gggatttcac gggcaaccgg agcaagcgga
    24661 tcttcgacga tccggtcggc aagcagtgcg gccgccacga aatgcctttc ctgatccaga
    24721 cctaccggcg cgacaccggc gagatcatgc acgatatctc ggcgccggtg tatgtacagg
    24781 gccgtcactg gggcggcttc cgcatcggct acaaggcctg acgcgcgacg atttgatgca
    24841 cctccctgcg agtgttcagc ccgccccggc gggctgcttt tttagtggtg cgcggggcta
    24901 cggtaggtgc cggattcctc aagaactttt ggcacctgcc gttatggcaa cagcaggcgg
    24961 ccagggccga tggaaggcca acgcgccgca acggagatca acatgtccga acttctcaag
    25021 aacatcgacg cccgtacgcg tctggccggc accaacaagc tggagatcct gctcttcacg
    25081 ctgggagtcg atgcccgcac cggccggcgc gaaaccttcg ggatcaacgt cttcaaggtg
    25141 cgtgaggtga tgcgtacgcc ccccatcacc gccgcaccgg agatgccggc ggcggtggaa
    25201 gggatggtca gcctgcgggg tgtgctggtg ccggtggtgg atctggccaa gtacgccggt
    25261 atgggcgccg acagcgcccg cgagatcatg atcgtcaccg agtacaacgg gcacacccag
    25321 ggcttcctgg tggaggcggt cgacacgatc ctgcgcctcg actggtccca gatgcgggtg
    25381 ccgcccgaca tgatgaccgc caacatgggc ggactcgtga ccgccgtcac cgagctgccc
    25441 ggccccaggc tggtgatgat gctcgacgtg gagaaggtgc tgtccgagac gacccgttac
    25501 gacgatgacc tgatgttcaa gggtatcgag cccatcaagt gcgaagagcc ggtgacggtg
    25561 tatttcgccg atgactcgtc ggtcgcccgc aagcagatcg agcggaccct cgatacgctg
    25621 ggcgtgcgtt acgtcggatc ggtcaatggc cgggcggcct gggacgaatt gcagaagctg
    25681 gcccactacg ccgaatcggc cggccgcaag gtcaaggatc ttctccagat ggtgctgacc
    25741 gacgtggaga tgccggaaat ggacggctac atcctcacca agaagatcaa gtcggacccg
    25801 cgtttcgagg gcgtcccggt ggtgatgcat tcatccctgt cgggcatgtc caaccagcag
    25861 ctgggacgat ctgtcggcgt cgacgaatac gtgcccaagt tcgagccgca acggctggcc
    25921 gaggtgctgc ggcgcctcgt cgaccggtgt caatccgccc gcgcggcgtg agtctggagg
    25981 ttcccaatgg atacggttct tgccgaaaga ggtctcctcg aggccgtcga tgcccgcacc
    26041 aagctggcgg gctccaaccg catggaaatc ctgctgttct ccctgggaac gcaggagatc
    26101 ttcggaatca acgtgttcaa ggtgcgcgaa gtgtcgcgca cgcccttcat caccaaggcg
    26161 cccaacatgc ccgatggcgt ggaagggctg atctccctgc ggggcaatgt gatcccggtg
    26221 ctggcgttgg ccaagatcat gggcatcgcg gcgcccggcg agccccttgg cggttccatg
    26281 atggtcaccg aatacagcaa gcgcaccctg ggtttcctgg tgcacgaggt ggaccggatc
    26341 atccgggtcg aatgggacaa ggtccgcgcg ccggacaacg tcaccacctc cggcaatcat
    26401 ttcatcaccg ccatcaccga actggccgac ggcaagctgg tgtccatcct cgatgtggag
    26461 accatcctcg cgtccacctt cggcgaggcc gtggtggggc agatccatcc gctggagggc
    26521 ggccacgacg tcaacgtgtt cttcgtggat gattccgcgg tcgcccgcaa gaagatcggt
    26581 gaaaccctgg acaaactcgg ggtcaaacac aagcacgccc tcaacggcct ggaagcctgg
    26641 acccgtcttg aaggcatggc gtcccacgcc cagcaacggg gcgtctgtct ggccgacgaa
    26701 ctcagcctga tcctcgtcga tgcggaaatg ccggagatgg acggctacgt cctgacccgc
    26761 aacgtcaagt ccgatgcccg cttcgagggc attccggtcg tcatgcactc gtccctgtcc
    26821 tcggaggcca accgggccat gggcaagagc gtcggggtcg attcctacgt cgccaagttt
    26881 gatgcagaca tccttgccga aacccttcgc ccgctgttgc tccgggcgca acgggactga
    26941 acggagacca cccacatgtc ggatcccaaa atgaaatttc tcgtcgtcga cgatttctcc
    27001 acgatgcgcc gcatcgtgcg caacctgctc aaggaactgg gcttcaccaa tgtggatgag
    27061 gccgaagacg gtcagatcgc gctgcagaag ctcaattcgc tgcctttcga tttcgtcgtg
    27121 accgactgga acatgcccaa tatggacggg ctgaccctgc tccagaacat ccgcgcgacg
    27181 cccagcctca agcacctgcc ggtgctgatg atcaccgccg aggccaagaa agagaacatc
    27241 atcgccgccg cccaggccgg cgccagcggc tacatcgtca agccgttcac tgccgcgacc
    27301 ctgtccgaaa agctggaaaa gatcttcgaa aagatgggca agaaggcggc ctgagcggcg
    27361 cccgcacgaa ggagcgtttc aaccatgtca aagcgtctca aattcgacga atcgggcgac
    27421 agtgacgaac ttcaggccct gttcgacagc atggccggag tggccgcgcc ggcatctccg
    27481 ccgccggccc cgcccaagct ggaagtggtg gcgccggccg acaaggccgg tgacgatgac
    27541 gatctgcagg ccctcttcga ctccgtatcg agcgcctaca cgccgccggc ggtggagggc
    27601 atcgcgtcgg agcacagctg cgacgtggtc ttcaaccgca tcgggacgat gacccggcgc
    27661 ctgcacgaca cgctgcgcga gcttggctac gaccgcagcc tgcaggaggc cgcccacgcg
    27721 attccggatg cccgccagcg cctcagttac atcgccgaga tgaccgagca ggcggcgagc
    27781 aaggtgctca acgccaccga tatcgccaag ccgatccagg acaccgtgga atccggggcc
    27841 accgccctgc gggcgcgctg ggatcagctc tacgccaacc agctgtcgcc ggatgaattc
    27901 aaggccctgg cgggcgagac ccgcagcttc ctcggcgatc ttgtccagca ttcccgggcc
    27961 accaacgccc agttgctgga gatcatgatg gcccaggatt tccaggatct caccgggcag
    28021 gtcatcaaga aggtggtgga gcttgcgcag ggcctggaga gccagctgct gcgcgtgctg
    28081 atcgaggcca tgccggccga acgcaaggcc gaggcgccgg aaggcctgat gaacggtccg
    28141 gtcatcagcg ccgtgggccg tgacgacgtg gtcaccagcc aggaacaggt ggacgacctc
    28201 ctggaaagcc tgggcttttg atcatccgca aggagcgacg acaatgagcg atttcagcgg
    28261 aatggaagat ctgttgcagg atttcctcgt ggaagcgggg gacctgctgt cgggggtgga
    28321 caacaagctg gtggatctcg aacgggcgcc cgacgaccgg gggctgctca acgacatctt
    28381 ccggggcttc cacaccatca agggcggcgc gggcttcctc aacgcgtccg agctggtcac
    28441 cctgtgccac ctcaccgaga acctcttcga caagctgcgt aacggcgaac tggccgtgac
    28501 gccggagacc atggacgtga tcctggcctc caccggcgcg gtgcgcgaca tgttcggcta
    28561 cctggagcag ggcgtgcagc ctgcggcagc cgatccgcag ctgatcgagg atctgcgtct
    28621 ggccattgcc ggtgcgctgc gtccggccgg cgttgtcacg tcggcgcccg aggtcctgcc
    28681 cgccgtggtg gcggcaccgc cgacgccggt gacgccggca cccggcggcg ccgacgggcc
    28741 agactggaac gccctcttcg atgcggtcac cagcaaggcg ccggcgacgg cgacggcagt
    28801 ggtgccggct gccgatcctc cgcccgtgca ccaatcggcg gaagatctgg cggcaaagtc
    28861 ccccgagcag gtcatcaagg ccgccgtcgg tcggcgggcc accgacaagc ccggggcctc
    28921 cggtccgatc ggccggcgtg agggcgagcg tcagcgcgac aactccatcc gtgtggatac
    28981 ggcccgcctc gaccaggtgc tcaacctgtc cggtgagatc ggtctcacca agaaccggct
    29041 caccgcgctg cgcagcgaca tcctcaacgg acgcgccgat acagagaccc tgcatgccct
    29101 cgatctggcg gtgagccagc tcgacctgct ggtttccgac ctgcagaacg cggtgatgaa
    29161 gacccgcatg cagcccatcg gccgcctctt tcagaagtac ccgcggatcg cccgcgatct
    29221 ggcccgcaac ctgggcaagg atgtggagct ggtcctgtcc ggcgaagaga ccgagatcga
    29281 caagaccatg atcgaggatc tctccgaccc catcatccac ctgatccgca acgcggtgga
    29341 tcacggcgtc gaggagacct ccgagcgccg tgccaacggc aagcccgaga aatccatcgt
    29401 acgtctcgaa gcccgccagg agggcgatca catcgtgatc ctggtggccg atgacggccg
    29461 tggcatgaac gccgaacggc tccgcgccaa ggcgctggag aaaggcctca tcagcgatga
    29521 ggaggccaac accatggacg agcgccagag ctacaacctg gtgttcctgc ccggtttctc
    29581 catggccagc aaggtgtcgg acgtttccgg ccgcggcgtg ggcatggatg tggtgcggac
    29641 caacatccag aagctcaacg gttccatcga catccgctcg tccctgggca agggcaccac
    29701 cttcatcatc agcctgcccc tgaccctggc aatcctgccg gtgctgctgg ttcgcctggg
    29761 ggatcagccc ttcgcggtgc cgctgtccat ggtccgcgag atcctgccca tcgagccggc
    29821 ggaggttcag gaagtgggcg ggcgcgccac catggtggtg cgtggcgagg tgctgcccat
    29881 catgccgctg gccaccctgc tggggtggga ccaggtgcag acaccggaat acggggtgct
    29941 catgcagagc gcggagcaga gcttcatcct cgccatcgac agctttgccg ggcgcgagga
    30001 cgcggtcatc aagtccctgg acgatttccg tcccaagggc gtggccggtg tgacgaccct
    30061 gtccaacggt cagatcgtcc tgatcctcga catgaaggag ctgctcggcg ctctcggcga
    30121 cacccgcggc atctcgcgac gggctttgct ggcgcatctg ccggtaccgg cgctggccgc
    30181 ttgatccgtc acgaccgccc gttcgcgggc ggtcggtgtc acacaacgct acaaaatccc
    30241 gtgacgtaga tcgctctatg ggaggccgtg cttcgttagc atggcctcga atgccttgcg
    30301 cacgcgaccg atggaggtcg cccggcggtg gggtggagag cgatcatggg tggtttcgag
    30361 tttgacgact ggcggtttct tgcggaaaac gatcccgcgg ccttcttccg cgcgcgggaa
    30421 cggaccctgc ggcagttcat cacgcaacac ccggaccagc gcgacgccct cggtgccctg
    30481 cagatgcgca tcgataccgc ccgtgccatg tccggtacgc cgcaacaggc ctgccgtgac
    30541 ctgttcggtc tgctcgagga gcacctgctg ctgctctccg aacggctggc cgatctccgt
    30601 cgggaagcga cgacgctgca acgcatgctg ggcgtggacg gcgccgaatt ggcggtcgaa
    30661 tccccctctg ttcggggcca ttcctgagcg ttcggcggcg catcatgggc gtccctgaga
    30721 tgcccgcgac ggcgccccga tggccgacga cagcgacctc gaaaagacag aatccgcctc
    30781 agcgcgacgc ctggagcagg cccgtgaaga aggtcaggtt ccccattccc gcgagctggc
    30841 caccttcatc gcgctgatgg ccggcgtcac ggcgctgtac gttctgggcg actgggcggg
    30901 gcagcggatg ctccacctgt tccgcagcgg gctgaccctc gaacgcagcc gtgcctttgc
    30961 cccggatgcc atggggcaga tcttcgaaca gctggccctc gacgcgcttc tcaccctggc
    31021 accgctcttc gtcgtcctca tcgtggcggc gttggtgacg cccttcctga tgggcggctg
    31081 ggtctttacc accaaggtac tcaccttcga cgtcagccgg ctcgatcccc tgcaggggct
    31141 cgggcgcatg ttttccaccc atggcctggc ggaactggtc aaggccagcc tcaagtcggt
    31201 gatcgtcgcc agcgtggggg tctgggtggt gtggcgggag cgcgatcacc tctttgccct
    31261 catgctccag ccagtggagg agagcgtcgg cgagtttgcc agcctcctgt tgctgtcgtc
    31321 gctgctcatc gtggtgggcc tggggatcat cgcggccatg gacgtgccgt accaattgtg
    31381 ggagtaccac cgcaagctgc gcatgaccaa ggaggagctg cgccaggaaa tgaaggagca
    31441 ggagggcgat ccgcagatca aggcgcgcat ccgcgcggcc cagcgggaga tgtcccgccg
    31501 ccgcatgatg agccaggtgc ccaaggccga cgtggtggtc accaacccga cccattattc
    31561 ggtcgcgctg cactacgatc cggacaaggc cggcgccccg gtggtggtcg ccaagggcgt
    31621 ggatctggtg gccctcaaca tccgcgaagt ggcccgcgag cacggcatcc ccattctcga
    31681 ggcgcccccc ctggcgcgcg ccctctacgc gcactgcgag ctcgaagacc cgattccggc
    31741 caccctttac accgtcgtgg ccgaggtgat ggcctatatc ttccagctca accactggat
    31801 cgccgaaggc gggctgccgc cggaggtgcc caccggcctg acggttccgg aaggcctgga
    31861 tccggcgggc accggggagg tgtctgcctg aagggtgggg cgtcgcagcg cccgccgtgt
    31921 gtccggggcc ccctgggcga tcatcggagg tggcgcccgt cgggcgaatg atcccggtct
    31981 gcgccgccgt gattcatcgc ggggcgcatt catcgggatg cccgtcatcc catgacggct
    32041 gcaggcgcgg cggttggcct cgtctcaggc ttttcggccg gtttgcgaga gggccgggcg
    32101 catgatccgg ccgtccggcc aacggcgcct ttctgccggc gccgcaccgg ttgccggggc
    32161 acgcgggacg gcaggggccc cacgctgacg tccggtgcag cccgcgccgg gtgcttcggc
    32221 gatgaccgct tcaaggtcgc cctggacggt caggtgatct gcgcctgcat cgtcggtgcc
    32281 tgcggcaccc acggcccgga agcgatcggc gccgggtcgg gcgcgccgca ttacggtcct
    32341 tccgaccggt ttgccagcag cgggtccgat gccacctatg cggtgaatct ggcccctggt
    32401 gatcttgtcg ccagcaccca cacctcgatt tcgtcgcccc gtcggcggag ggcggccggc
    32461 cggtcgggac gaacgcttcg cgctcgacaa ggtgagtgtg aacggccgcc tcgggacgcc
    32521 caacacgcct gcgcccgagc cttcggtgtt cgcgttgctg ggaatcggat ggacgagggt
    32581 cgcgcagacc cgccggtctg agcgccaggg ctgaagggcc tgaaggtctg gagcgcgctc
    32641 cagcgtcccg catcggccgc ggcatcgtct accgggcgca gcggccgtgt gcggtgtgga
    32701 atggcccgtc ttcggtccgt gggtcgttcc gccccgaata gccccccctt tccgcctcag
    32761 ttccggcctt ctcccgcggc gtgtcgcggg caccatggcg ggcatgaacg atagcctgaa
    32821 cctgcgtgcg ctcttcgcac cggccaacat ccgccagttg gccgggccgc tgctcgtcgt
    32881 gctgattctg gcgatgatgg tcctgccgtt gccggccttc gtcctggacc tgatgttcac
    32941 cttcaacatc gccgtctcgg tgatggtcat gctggtggcc atgtacaccc gcaagccgct
    33001 ggatttctcg gtcttcccga cggtgctgct ggtcaccacg ctgctgcgcc tgtccctcaa
    33061 cgtggcctcc acccgcgtgg tgctgctgga aggccacacc ggcccggacg cggcgggcaa
    33121 ggtgatcgag gccttcggtc acttcctggt ggggggcaat accgcggtgg ggctggtggt
    33181 cttcatcatt ctcacggtca tcaacttcgt ggtgatcacc aagggtgccg gccgcatcgc
    33241 cgaagtgggc gcgcgtttca ccctggacgc catgcccggc aagcagatgg ccatcgacgc
    33301 cgacctcaac gccggcctca tcgacgagaa ggaggccaag cgccggcgcg ccgagaccgc
    33361 ccaggaggcc gacttctacg gcgccatgga cggtgcctcc aagtttgtcc gcggcgacgc
    33421 ggtggcgggc atcctgatca tgggcatcaa cgtggtgggc gggctgatcg tcggtgtcat
    33481 ccagcacaac atggaactgg ggcaggcggc caacaattac accctgctga ccatcggtga
    33541 cggcctcgtc gcccagctgc cggccctcat catctcggtg gcggcgggtc tggtggtgtc
    33601 ccgggtgggc gacgaagggg acatgggcag ccaggtggtc ggccaggtct tttccaaccc
    33661 ccaggtgctg gtcctcaccg ccgccatcct cggcgtgctg ggcctcattc cgggcatgcc
    33721 caacttcgtg ttcctgctgc tcgccggcgg gctcggtgcg ctcgcctggt acggcggcaa
    33781 gcgtcaggcg gcgattgccg aagaggaggt gccggccgcc gccgcgccgg ccgtgccgcc
    33841 cgaggcccag gaggccagct ggaccgatgt gacgccggtg gacgtcctgg gtctggaggt
    33901 gggctatcgc ctgattccca tggtggacaa ggcccaggat ggcgagctcc tcaagcgtat
    33961 ccgcggcctg cgcaagaagt tcgcgcggga catcggcttc ctgtcgtctg cggtgcacat
    34021 ccgcgacaac ctggagctca agcccaacgc ctaccgcatc gtcctcaagg gcgtcgaggt
    34081 gggcaacggc atggcctttc cgggacagtt catggccatc aatccgggcc gcgtgagtgg
    34141 ccccctcaac ggtcgcgaga ccaccgatcc ggccttcggc ctgcctgcgg tgtgggtgga
    34201 tgccggtcag cgggagcagg cccacgccct cggctacacg gtcgtggacg ccagtaccgt
    34261 ggtggcgacc catctcaatc acctgatcct cagccacgcc gccgagctcc tggggcgcac
    34321 cgagacccag gccctgctgg accacatcgc caaggacaac cccaagctgg tggaagacct
    34381 ggtgcccaag ctgctgcccc tcgccaccgt ccagcgggtc ttgcagaacc tcctcgacga
    34441 aggggtcaac atccgcgaca tgcgctccat cgtcgaggtg ctggccgagt gcgcgccgcg
    34501 cgtgcaggac ccggtggagc tcaccacccg cgtccgccag gcccttggcc gcgccatcgt
    34561 gcagggactg ttcccgggca acggcgaaat ccaggtcatg gccctcgatc caggcatcga
    34621 gcgggtgctc ctgcaggcca ttggcggcgg cgggccggag ggcggcgcca tcgagccggg
    34681 cctcgccgac accctgctgc gggagaccgc cgtcgccgcc cagcgcatgg aggacatcgg
    34741 cctgccgccg gtgctgctgg tgccaccggc cctgcgctgg ctgctgtcgc gcttcctgcg
    34801 ccgcagcgtc ccgaccctca aggtcgtctc caatgccgag gtgccggaga cccgcctgat
    34861 tcgcgtggcc tccatgatca ccgtggcccg ctgaacgccg tccggtacgc ccaacacggg
    34921 aatagcgccc taaaacctgt cttttcccgg ccatcgaatg gcgcccgtct cccgataatc
    34981 ggaggcatgg aaatggatcg catccctgcc ttgtgagcca tcgcaccccg gagaccggag
    35041 catgaacgtc aagcgctact tcgccaaaac cgcccgcgag gcgctgcgcc aactcaagga
    35101 agagctgggg cccgacgccg tcgtgctgtc caatcgcgcg gtgaatggcg gggtggagat
    35161 tctggcgctg ccggccaacg acgtggcggc cctgcagcgc gcgcgccagc aggcggcggc
    35221 cgcggcgcct gctgccccgg tgatgcccgc tgcggcttcg accgaggtgc cccgtgcggc
    35281 ggcccgggcg tcccagccgg cggccgactt cttcgacgac gatttccgcc tgagtctgtc
    35341 ccgcgccgcc gcggcccgta cccagcccgc agcggcgcca gccgccgcgg gctgggtacg
    35401 gg
//
LOCUS       DAOQPA010000024        34712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_518_length_34712_cov_19.680151, whole genome shotgun sequence.
ACCESSION   DAOQPA010000024 DAOQPA010000000
VERSION     DAOQPA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 34712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34712
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_34712_cov_19.680151"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKX01_05310"
     CDS             complement(<1..796)
                     /locus_tag="PKX01_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ75575.1"
                     /translation="MPLLTLDDACLAFGHVALLDKADFQLDAGERVALIGRNGSGKSS
                     LLKALAGQATLDDGRVWRQPGVRIAYVPQEADFSLDLSVFAAVAEGLGDVAQLLVHYH
                     AAMLAVADEASDAALAHLEAVQHRVEDAQAWRLEQRVEQILGTLRLAGDAPVGSLSGG
                     GIKRVALARALVGEPEVLLLDEPTNHLDIDGILWLEALIREFPGAVVVITHDRVFLDH
                     VATRIVELDRGLLSSFPGRYADYQVRKADQLDAEEKANARFDKLLAQ"
     gene            1000..1185
                     /locus_tag="PKX01_05315"
     CDS             1000..1185
                     /locus_tag="PKX01_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HMZ75576.1"
                     /translation="MSPALSPSSQTTNDDARRESASPVTLRSADLLQDRGCVLIEHQG
                     MVYALRATRAGKLILTK"
     gene            1182..1325
                     /locus_tag="PKX01_05320"
     CDS             1182..1325
                     /locus_tag="PKX01_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75577.1"
                     /translation="MIFVAPSRQPGAFSTRPMSLLGVAALLATGLAGSLGSLFLVLHF
                     YAS"
     gene            1345..1581
                     /locus_tag="PKX01_05325"
     CDS             1345..1581
                     /locus_tag="PKX01_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMZ75578.1"
                     /translation="MYVCVCRAITERHITEAVNDGARRLRDLRRDLGVTEECGRCARC
                     AHACLKEAIDQIGPVSILSGCRTESTYSLSQEAA"
     gene            1578..2054
                     /gene="bfr"
                     /locus_tag="PKX01_05330"
     CDS             1578..2054
                     /gene="bfr"
                     /locus_tag="PKX01_05330"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HMZ75579.1"
                     /translation="MKGDKKVLQHLNKQLTIELTAINQYFLHARMYKNWGLGKLGKHE
                     YEESIEEMKHADALIERILFLEGLPNLQNLNKLLIGENVPECLQGDLKLELGGQAELK
                     EAIAYCETVRDYVSRELFESILEDTEEHIDYLETQIELIDKVGLQNYLQSQMDVPS"
     gene            complement(2151..2657)
                     /locus_tag="PKX01_05335"
     CDS             complement(2151..2657)
                     /locus_tag="PKX01_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HMZ75580.1"
                     /translation="MMGDERDVLRPEPLTSAAFAPFGEVIEAASARQRILINEGTAER
                     FHDLARIEPGADGRAIVSIFRGQPRPMPFPVSMLERHPLGSQAFVPLSGRPYLVVVAH
                     GRDTPDEARLRVFLAADGQGVNFAPGVWHHPLLTLDEVSDFLVIDREGPGLNCEEKSL
                     TRPVHVAP"
     gene            complement(2654..3694)
                     /gene="alc"
                     /locus_tag="PKX01_05340"
     CDS             complement(2654..3694)
                     /gene="alc"
                     /locus_tag="PKX01_05340"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HMZ75581.1"
                     /translation="MTTHSFDPPRIAAPAVLPDWARRSVNLADSRIGARALFATDEFF
                     APLERMLNPEPAVFVPGQYDANGKWMDGWETRRKRTTGYDWSLVRLGRRGIVRGFDID
                     TSHFTGNFAPAVSIDATDCESDEPDLLVRARWTRILPSTSLTGNSHHLLEIESSDTWS
                     HLRINIYPDGGIARLRVYGQPVGVFDTSDPEREYDLVALENGGRAVAWNDAHFGSAAN
                     LIAPGRGINMGDGWETRRRREPGNDWCILQLGAPGVVDRIEVDTAHFKGNFPDRCSIQ
                     AARMDGGTDQSIITQSMFWPVLLPEQPLGMDALHVFREGIAGLGPVTHVRFNIIPDGG
                     VSRLRLWGRVTR"
     gene            complement(3691..4632)
                     /gene="puuE"
                     /locus_tag="PKX01_05345"
     CDS             complement(3691..4632)
                     /gene="puuE"
                     /locus_tag="PKX01_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HMZ75582.1"
                     /translation="MSPIHNSEYPRDLVGYGAHPPHADWPGRARVAVQFVLNYEEGGE
                     NCVLHGDPGSEQFLSEMFNPAAYPDRHLSMESIYEYGSRVGVWRILREFERRGLPLTV
                     FGVGMALQRHPELTAAFVALGHEIACHGWRWIHYQNVDEATERAHMAQALDVIEDMTG
                     TRPLGWYTGRDSPNTRRLVADDGRLLYDSDYYGDDLPFWVQVTRSDGVRVPHLIVPYT
                     LDTNDMRFALPQGFSHGDPFFQYLRDSFDVLYAEGDERPAMLSIGMHCRLLGRPGRFL
                     ALQRFLDHLARHDRVWICRRIDIARHWIAHHPWRAPA"
     gene            4683..5198
                     /gene="gpt"
                     /locus_tag="PKX01_05350"
     CDS             4683..5198
                     /gene="gpt"
                     /locus_tag="PKX01_05350"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HMZ75583.1"
                     /translation="MERSPNSPYRDDIVISWPELHRDTRLLCHQLMEKGPFKGIIAIA
                     RGGLIPAALVARELEIRLIDTICAASYEADNDDTAQTVRREVKILKGIEHDGDGYLLI
                     DDLVDTGNTARVIRAQLPKAYFATLYAKPMGRQVVDLCIKEFSQDKWIYFPWDIDYQF
                     VPPIKKHHRPA"
     gene            complement(5199..6386)
                     /locus_tag="PKX01_05355"
     CDS             complement(5199..6386)
                     /locus_tag="PKX01_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HMZ75584.1"
                     /translation="MDAAYLIDVGSLLLRWLHLIVGIAWIGSSFYFVWLDNSLKAPTD
                     PELIRKGVGGELWAVHGGGFYNPQKYLVAPQHLPEELHWFKWESYSTWITGFFLLGVL
                     YYSQAATYMVDPAKAALSPAAAIGIGLATLAGGWLVYDGLCRLLINRSLLLFAALYFA
                     FVVAVAWGLTHLLSGRAAFLHVGAMIATTMSGNVFFWIIPGQKTTVAAMQRGETPDPI
                     HGKRAKQRSVHNNYLTLPVLFCMISNHYAFTYNHPQAWRVLAMILLAAVLIRHFFNLR
                     HKGQVAWQYPALGAALLLAVFVELAPRHAAPAAGSPAASLADIQPIVVARCTGCHAAR
                     PTLIPAAPAGVLLDSAEAIRANGPRILAQAVQLKAMPLGNVTGMTDSERERIAAWAAG
                     GYR"
     gene            complement(6386..6739)
                     /gene="uraH"
                     /locus_tag="PKX01_05360"
     CDS             complement(6386..6739)
                     /gene="uraH"
                     /locus_tag="PKX01_05360"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HMZ75585.1"
                     /translation="MGRLTTHVLDTAAGVPAAGMHFQLYRLDGDCRTLLSAGQTNSDG
                     RSEAPLLSGETLLPGQYELAFAVAEYFRNRGVALPEPPFLDRVPLAFGIADPAGHYHV
                     PLLVSPWSYSTYRGS"
     gene            6961..7482
                     /gene="uraD"
                     /locus_tag="PKX01_05365"
     CDS             6961..7482
                     /gene="uraD"
                     /locus_tag="PKX01_05365"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HMZ75586.1"
                     /translation="MPSRFTPAQLSDLDRADFIAAIGGVFEHSPWVMARAWEMRPFSS
                     RADLDAALVKVIREAGRDELLALIEAHPELAGKAAIRGDLTADSAREQAGAGLNACSP
                     EEFELIQRLNRAYRARFGWPFIVAVKGMTRHDIIAAIERRLPQTSTEEFVEALAQILR
                     IAGFRLDELLTDA"
     gene            complement(7528..8514)
                     /locus_tag="PKX01_05370"
     CDS             complement(7528..8514)
                     /locus_tag="PKX01_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ75587.1"
                     /translation="MATTCSWESIDVHLLQVLHALLTECSVSNAARRLNQSQPAVSTA
                     LRRLRDITGDQLLVRSRNGMTPTERGMALLEPVKVALAQIEAIGLQQIRFDPMKSRRT
                     FNIATPDYLNAILIGNIVARLRRLAPNSQVVLHSFGPDYDYAKALESGELDTVIGNWP
                     QPPEHLRLAPLFDDDVVCLMRRSHPLAGRTLSVDDYLNAEHLVPTPYAVGQRGVIDMQ
                     LARERLKRNVVASVPYFNLVPYVLLQNDIIFSAPRIFAEYCCSLGELCFSESPLDFPK
                     MRFYLLWHDRAHHSEECRWFRDQIIEVIREKTGAPAAAGTRVPAQVATPATV"
     gene            complement(8784..9623)
                     /locus_tag="PKX01_05375"
     CDS             complement(8784..9623)
                     /locus_tag="PKX01_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75588.1"
                     /translation="MLVKKTALGCAMLIAGLSCAHAADWSDTYVGWRYGTKFAEPYNP
                     DDIKKNIFNLGHVSGYKYGTNFLNVDMLLSDSKDSFNNGGGAQETYVVYRHTFDFGKI
                     AGNPDAFKFGIVRGLGFTVGFDYNTKSGDSYQSRKRMPLAGPTVMFDVPGFLNLSVLQ
                     LWESNAPRTHPSRYSYDAHPMLTLAWGIPLGSLPLTFEGFMNYIAAKGANEFGGPTKP
                     ETNFDGQIMYDASPLVGARPKTFKVGVEYQYWRNKFGNDHTGAAGNGAFAKTPMLRAE
                     YHF"
     gene            10021..11049
                     /locus_tag="PKX01_05380"
     CDS             10021..11049
                     /locus_tag="PKX01_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ75589.1"
                     /translation="MPIPFNAGRRLVLAAAMSLLAMPAFADAPTRIKFTLDWRFEGPA
                     APFLLAKAKGYFAAEGVDMEIDSGSGSAGAVTRVATGAYEMGFADFNALVEYDAKTPG
                     SKIQGVYMVYNATPAAVFVLKKSGITKPADLAGKTLAAPIFDAGRKAWPAFAKSNKLA
                     VDAVKWQTVEPALRETLLARGDVDGITGFYFTSLLNLEARGVKSDDIVALKYPDFGVE
                     LYGNAIIASPKLMAENPKAVAGVLRAFNKALKEVIANPAAAIAYVKERDPLINADLET
                     RRLKLALDSSVVTPEVKLNGLGAVTPERMQRSIAETAEAYALPKVPTAADLFNPNFLP
                     SKTDRTLK"
     gene            11111..11890
                     /locus_tag="PKX01_05385"
     CDS             11111..11890
                     /locus_tag="PKX01_05385"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75590.1"
                     /translation="MSFVTFEDVSLAYTPGGANAIENVSLSIREGEFVAVVGPSGCGK
                     STLMKLVSGLKPPSAGYVYVDGREVGGPLKIVGMAFQAANMLPWRTTLDNVMLPLEIV
                     EPYRSRLRRERETFKADAEALLARVGLAGFGQKYPWELSGGMQQRASICRALIHQPRL
                     LMLDEPFGALDAFTREELWCMLRDLWQQQPFTVVLVTHDLREAVFLADTVYVMSKRPG
                     RILIRKEIDLPRPRPLDVTYTDAFAGYVHELREHIGRIRES"
     gene            11902..12678
                     /locus_tag="PKX01_05390"
     CDS             11902..12678
                     /locus_tag="PKX01_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75591.1"
                     /translation="MSLKQSTLVKSAPWLLTAVLFIVWELICVGFAIPDYLLPAPTAI
                     WASGLENAAPIMMHATQTLMTTLAGFALAVVVGMVLGLAVGASPVVYKATYPLLVGFN
                     SIPKVAFVPVLVVWFGIGTIPAILTAFLISFFPVVVNVATGLATLEPELEDVLRSLGA
                     HRWDIMVKVGLPRSLPYFFASLKVAITLAFVGSVISETVASNLGIGYLMMSASSSMNM
                     PLVFAGLIVIGVMGVVMYELFALLERNLTGWAHRTPGAAT"
     gene            complement(12787..14112)
                     /gene="guaD"
                     /locus_tag="PKX01_05395"
     CDS             complement(12787..14112)
                     /gene="guaD"
                     /locus_tag="PKX01_05395"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HMZ75592.1"
                     /translation="MTHRTVPDRLILGQILHFLDDPGEGDDPHSYAHFPQGALWIVEG
                     RVAAVGHRRAVESAIAPERLAAAERFDYGDRLVLPGLVDTHCHYPQSAVIASFGRQLL
                     DWLNTYTFPAEMAFRDPEVARATARYFLDRLLAHGTTTASVFATVHAASVEAFMAEAD
                     ARQLRMLTGKVMMDRHAPPELCDTVADAERDCVALIERWHGRGRLRYTITPRFAPTST
                     SAQLALAGELYASRPDLHVQSHLAENPDEIAWVKALFPECRDYLDVYARHGLLGPRTI
                     YGHCIHLSGAERAEMAATGTAAAFCPTSNLFLGSGFFDHAASVEAGLRVGLATDVGGG
                     SSFSLIRTLDEAYKVSQVHRRPLSPLRAWYLATLAGARALYLDAFIGNFEVGKEADCV
                     VLDERATPELEFRLRGVTGLAERLFALTVLGDERCVVATHVLGAPAFQR"
     gene            complement(14122..15483)
                     /locus_tag="PKX01_05400"
     CDS             complement(14122..15483)
                     /locus_tag="PKX01_05400"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HMZ75593.1"
                     /translation="MATLLLKDAEVLVTQDATRREIPGGGVFIRDGVVEAVGPSDQLP
                     DGADEVISLAGCVALPGLVNTHHHMYQSLTRAIPGAQDAELFGWLRRLYPLWAGLTPE
                     MIRVSTLTAMAELILSGCTTSSDHLYLYPNGSRLDDAIEAAAILGMRFHAARGSMSVG
                     ESAGGLPPDRLVEDEAAILKDTQRLIETWHDPARHAMRRIVVAPCSPFSVSRELMREA
                     AILARGHGVSLHTHLAENDNDVAYSREKFGMSPAEYAESLGWVGADVWHAHCVKLDGP
                     GIELFARTGTGVAHCPCSNMRLASGIAPIPAMRAAGVPVGLGVDGCASNDAAHLLGET
                     RQAMLLARVGYGPSAMSARQALELATLGGARVLGRDDIGALAPGMSGDVVAFPTDELD
                     LAGAAVHDPVAALVFCTPPKVKVSVINGQPVVREGQLVPLELPPLIAHHNRLARQLAD
                     HRA"
     gene            complement(15489..16409)
                     /locus_tag="PKX01_05405"
     CDS             complement(15489..16409)
                     /locus_tag="PKX01_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75594.1"
                     /translation="MIEHLVPVLAGAIAAATPLIFAGLGELVAERTGVINLGVEGMML
                     TGAIAGFAAAAESGGGAAAGLPAGALAGMATALLFAVFALSLAANQAACGLALTIFGI
                     GLSAFIGQHYVSYSLPGLAPVDIPLLADIPVLGPVLFHQDAVVYLSFAAFAGVAWILA
                     RTRLGLLLKAVGESPEAAHAVGYPVIAIRYGAVVFGGAMAGLAGAYLSTVYTPLWVQN
                     MSAGRGWIAVALVVFATWKPLRLLLGAYLFGGVTILQFHAQGLGLDVPVQLLAALPYA
                     ATVIVLVFISRDRRLLQLNLPASLGKSFLP"
     gene            complement(16416..17474)
                     /locus_tag="PKX01_05410"
     CDS             complement(16416..17474)
                     /locus_tag="PKX01_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75595.1"
                     /translation="MHLLEPRAQPSQLMRWTAPLIAIAATLLTGSLLFWALDKSPLTS
                     FEVFFVQPLTSSYGWSELLIKACPLILIAQGLAIGFKARIYNIGAEGQLIVGALFGGG
                     VAIHFNGSTSAWVIPAMVVAGAIGGALWGALPALLKTRFNAEETLTTLMLTYVATFIL
                     SYMVNGPWRDPEGMNFPQSIMFDDNALFGILLEGSRVNSSVFITLVAVLAFWLFNARS
                     FLAYQLEVGGQAPAAARYAGFSASQTVWFSLAMSGLTAGLAGVGEVAGPVGQLNLNIS
                     PGYGYAAIIVAYLGRLHPLGIVLAGALMALIYLGGEAAQVSLQMPAAITGIFQGMLLF
                     FLLGADAFIDNRLRRPSR"
     gene            complement(17461..19002)
                     /locus_tag="PKX01_05415"
     CDS             complement(17461..19002)
                     /locus_tag="PKX01_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75596.1"
                     /translation="MSTPEPRLQLLHITKRYPSVVANSDISLTVMPGEIHAILGENGA
                     GKSTLMKIIYGAVKADEGEVMWNGHQVAIANPAQARKLGIGMVFQHFSLFETLTVVEN
                     IALVLDEAFDLPALAKRVIEVSERYGLPLDPFRLVHSLSVGERQRVEIVRCLLQDPKL
                     LILDEPTSVLTPQAVLKLFDTLRTLAAEGVSILYISHKLHEIKALCDSATIMRGGKVT
                     GNATPRDETPASLARMMIGRELPVCEAPPFIGESRPVLQVSGLSLPSDDPFGTSLKDI
                     SLTVHAGEILGIAGISGNGQAELLAALSGETPVARDAICLEGAAIGHLDAGRRRDRGL
                     VFVPEERLGRGAVPSLSLARNALLTAHRQGMKRLGLVSYAKARAFAEQCIARFDVRCG
                     GAASAARSLSGGNLQKFIVGREIMLSPKVMIVAQPTWGVDVGAAAFLRQTLLDLSRTG
                     VAILVVSEELEELFEISDRIAVLAAGKLAEPEPKAATNAEKVGLQMSGLFTAAQSGES
                     VHASA"
     gene            complement(19005..20018)
                     /gene="xdhC"
                     /locus_tag="PKX01_05420"
     CDS             complement(19005..20018)
                     /gene="xdhC"
                     /locus_tag="PKX01_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HMZ75597.1"
                     /translation="MTTRAPDWLKALPAVLEAGQSVELVTVARADGSTPREAGAAMLV
                     GRETIVDTIGGGHLEWEAISHARRRLAVGGGRPEIVRYNLGARLGQCCGGVVWLVFES
                     MPASERDAWVERRASVLAGHHLHRQVDSEAAASVWQRAPVAGGETRLGGTAADWRFAQ
                     HVGGLAFPIQLFGAGHVAQALVRQLQLLGAQITWVDTRDGAFDGLDVTDVRTVLTDVP
                     ESEVARAPAGSFFVVMTHSHALDFSLCETIFRRRDFAYFGLIGSRSKRRSFEHRLVDR
                     GLNPERLDELVCPIGIAGIVDKAPAAIALAVAAEIFQVHEARRLVGDAARPRLIDNPV
                     QRE"
     gene            complement(20015..22363)
                     /gene="xdhB"
                     /locus_tag="PKX01_05425"
     CDS             complement(20015..22363)
                     /gene="xdhB"
                     /locus_tag="PKX01_05425"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HMZ75598.1"
                     /translation="MSPSGATPVGRSVPHESAPLHVSGKAQYIDDLPEMAGTLHAAPG
                     LSTCARGRIRAMDLSAVRAYPGVRCVLTAADIPGENNCGPILHDDPIITGDLVEFYGQ
                     VIFVVAAETREAARQAARLARVEYAAEPPILDMDSAIAAESWVLPPFVMTRGAVEQAF
                     ENAPHRLSGAARLGGQEHFYLEGQISCAVPRESDTIHVFCSTQHPTEMQQMVAHALGW
                     RSHQVSVECRRMGGGFGGKESQSAQWACLAALLAVKTGHPVKLRLDRDDDMVATGKRH
                     PYQYRWESAFDDAGRILGLRLEMASDCGYSADLSGPVNDRTICHIDNAYYLDAVALRS
                     LRCKTNRVSNTAFRGFGGPQGMFVIETVLDDIARHLGLDALAVREANFYDVVPGARST
                     TPYGMTVEDNVAPALVAQLADASDYGARRAAILAFNASSPVIKRGLALTPVKFGISFN
                     ATHYNQAGALVHVYTDGTVLVSHGGTEMGQGLYTKVRQIVAHEFGLPLEDVRLSSTDT
                     SRVANTSATAASSGTDLNGKAAQAACQHIKARLAAFVVELVQGAVAVEQVRFVDARVQ
                     AGEASAPLFDDSFAALVMRAYRARIQLWDAGFYRTPKIHFDPQTKLGRPFYYFAYGAA
                     ASEVAIDTLTGECRVLRVDVLHDAGRSINPALDIGQIEGGFIQGMGWLTSEELWWRPD
                     GPQAGRLMSHAPSTYKIPTATDLPDVFNVRLFDNANVEDSIHRSKAVGEPPFMLALSV
                     FMALRDAVAAALPAGQSPRLEAPATPEAVLRALGCLAPEDGA"
     gene            complement(22372..23868)
                     /gene="xdhA"
                     /locus_tag="PKX01_05430"
     CDS             complement(22372..23868)
                     /gene="xdhA"
                     /locus_tag="PKX01_05430"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HMZ75599.1"
                     /translation="MPIRPIQFYFRGAVQSVQGPAPTRTVLQFLREDLHQTGTKEGCA
                     EGDCGACTVVIGELAKGPEGDELRLRAVNACIQFLPALDGKALFTVEDLSADGHLHPV
                     QQALVERHGSQCGFCTPGFVMSLYALYENTPICPPRAEIDHCLSGNLCRCTGYRPIVD
                     AARDAFLAPRRSLERAPIVAALKSLAELPPLDYAAAGRHSYGPRTLTELAELRERLPD
                     ARLVAGGTDVGLWVTKQGRDLGDVIQLGGVAELKRIRVGAGQLEIGAGVSLTDAFAAL
                     TAFEPHWDELARRFASTPVKNAGTLGGNVANGSPIGDSMPALIALGCRVVLQQGAHTR
                     EMPLEAFYLDYQKTALQRGEFIRALVLDSPLPPLNRQFRAYKVAKRFDQDISAVACGF
                     AVHLDDEHIVRGARLAFGGVAGTPKRADAGEAALLGRPWTPDTVRLAMAALREDFAPL
                     TDVRASAAYRLSVAANLLQRFWLETTAPPDGLPARLSALGPIQPEERS"
     gene            complement(23985..24479)
                     /locus_tag="PKX01_05435"
     CDS             complement(23985..24479)
                     /locus_tag="PKX01_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ75600.1"
                     /translation="MSDILTAADETFLRHCIELSRRVRDGGKHPFAAIVVDELGRIVA
                     EAGNDSVPPDGDPTRHAELVAAGLAARRLTPGQLSRATLYTSAEPCAMCAGAIYWCGI
                     GRVVYALSEHRLLGLTGDHPENPTFSLPCREVFARGQRRIAVTGPCLEDEAAAPHAGF
                     WTRE"
     gene            24640..25719
                     /locus_tag="PKX01_05440"
     CDS             24640..25719
                     /locus_tag="PKX01_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ75601.1"
                     /translation="MLLRRLKSFTAVAVMALAGLHAAHAAEPIKAGFVYIGPTGDHGW
                     TYSHDEGRKLLEAQSNGSVKTTFVENVPETADAERVFRDLAQKGNKVVFGTSFGYMNQ
                     MAKVAKAFPNTIFMHATGYKTAPNLGVYDVRTYEGAYMLGVVAGKMSKGNKLGVVASI
                     PIPEVIRNINAFTLGARSVNPAITTRAIWVNSWFDPGKEREAALALIAQGCDVLMQNT
                     DSPAVVQAAQEKGVLAFGWDSDMTKFGGKAHLAASVLNWGVIYKKTVDEVAAGTWKSG
                     DIWWGVKEGAVNIDNFGPAVPAPVKKLAEERRDAIKAGKLHPFTGPLKEQSGKEFLAA
                     GKIFGDGDLKKMNFYVEGVEGSIPK"
     gene            25829..26866
                     /locus_tag="PKX01_05445"
     CDS             25829..26866
                     /locus_tag="PKX01_05445"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HMZ75602.1"
                     /translation="MPPPLAADAALDFACRMPKAELHVHIEGTLEPELAFALARRNGI
                     ALPYADEDALRKAYAFDSLQSFLDLYYACADVLRTPDDFRDLMLAYLERAVADNIVYT
                     EIFFDPQTHTARGIPFETALRGLEDGVYHARRLWGIEGRLILCFLRHLSEEDAFATLA
                     EAEPFLERIHGFGLDSSERGHPPAKFARVFARCRALGKPIVAHAGEEGPPAYISEALD
                     LLGARRIDHGVRAVEDAAVVDRLIRDAIPLTVCPLSNLRLCVFPSMQDHTLGRLVAAG
                     ACVTLNSDDPAYFGGYLNDNIRAVHAAFAFDIPTWYQLARNSFQASFASPTEKQQWID
                     RLDAARAATGL"
     gene            complement(27141..27215)
                     /locus_tag="PKX01_05450"
     tRNA            complement(27141..27215)
                     /locus_tag="PKX01_05450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27180..27182),aa:Arg,seq:cct)
     gene            complement(27299..27487)
                     /locus_tag="PKX01_05455"
     CDS             complement(27299..27487)
                     /locus_tag="PKX01_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HMZ75603.1"
                     /translation="MNIAPTPSTSAANGADPVSIAMLRKTLDQQQANAQQLLQALPQP
                     AKAPDPTATVGRNVDVFA"
     gene            complement(27492..27977)
                     /locus_tag="PKX01_05460"
     CDS             complement(27492..27977)
                     /locus_tag="PKX01_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HMZ75604.1"
                     /translation="MPSFDITSEVDMVALRNAVDGANRKITGRYDFKGSDARVEQTEK
                     LLTLHGDSDFQLEQMLAILLPEMTAKKIDVRCLDHGDVQKVGGNKVKQELKVRVGIEQ
                     EQSKKIVKLLKDSKLKVQASIQGDAVRVSGAKRDVLQECIALVRKDITDYPLQYGNFR
                     D"
     gene            complement(27991..28314)
                     /locus_tag="PKX01_05465"
     CDS             complement(27991..28314)
                     /locus_tag="PKX01_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HMZ75605.1"
                     /translation="MTQNVQFDTVSVVKKANVYFDGKCVSHSIVLADGTKKSVGVILP
                     ATLTFNTGAPEIMEGVAGACRVRLKGETEWKTYGAGESFNVPANSAFDIEVSGEPYHY
                     VCHFG"
     gene            complement(28334..28567)
                     /locus_tag="PKX01_05470"
     CDS             complement(28334..28567)
                     /locus_tag="PKX01_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HMZ75606.1"
                     /translation="MDPSAPLFFGLTVAEFEDISLKLCVGGLIAYMVFIIGQLAWESK
                     AGKYGAIWMFVGLGVGFIGFIAKGLIQRLLGIE"
     gene            complement(28579..29808)
                     /locus_tag="PKX01_05475"
     CDS             complement(28579..29808)
                     /locus_tag="PKX01_05475"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMZ75607.1"
                     /translation="MSDVKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEPARAKALQFGIKPENIFIDDLREEFVRDFVFPMFRCNTIYEGEYLLGTSIARPLIA
                     KRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPNVKVIAPWREWDLLSREKLL
                     AYAEKNGIPIEMKHKQGGSPYSMDANLLHISFEGRHLEDPAAEAEESMWRWTVSPEAA
                     PDAAEYLDLEFEKGDLVALNGTRMKPHELLARLNELGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYTGYWWSPERLALQAL
                     IDQTQQTVNGWVRLKLYKGNVIVVARDSKTDSLFDPTIATFEDDQGAYNQKDAHGFIR
                     LNALRMRIAANARAKRG"
     gene            complement(29872..30795)
                     /gene="argF"
                     /locus_tag="PKX01_05480"
     CDS             complement(29872..30795)
                     /gene="argF"
                     /locus_tag="PKX01_05480"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMZ75608.1"
                     /translation="MKAPRHYLQFRDFSRAEYDYLFARTRWIKDKFKRYEPYHPLFDR
                     TLVMIFEKASTRTRLSFEAGMQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDVV
                     MIRTFEQSIIERFAEYSRVPVINGLTNEYHPCQILADIFTFIEHCGPIQGKTVAWVGD
                     SNNVCNTWLQAAEVLDFHVHVSTPPGYEVEPERAGLIGTGHFAHFDDPMEACRGADLV
                     TTDVWTSMGFEAENEERMRDFAEWRVDEDMMRVANPGAVFMHCLPAHRGEEVTAEVID
                     GPQSVVWDEAENRLHVQKALMEYLLIGKVES"
     gene            complement(30814..31989)
                     /locus_tag="PKX01_05485"
     CDS             complement(30814..31989)
                     /locus_tag="PKX01_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMZ75609.1"
                     /translation="MPHLMNTYGRLPVAFSHGQGSWLFDTEGKRYLDALSGIAVSTLG
                     HNHPRLVRAIAEQAAQVIHTSNLYAIPRQEQLSDRLAALAGMDEVFFCNSGCEANEAA
                     IKLARLYGHGKGIAEPAIVVMEHSFHGRTLATLSATGNAKVQKGFEPLVGGFVRVPFD
                     DLGALEQATAGRSDIVAILLEPVQGEGGIHVAHSAYMEALRGMCDANGWLLMFDEVQC
                     GVGRTGKWFGHQNAGVKPDVMSLAKGLGSGVPIGACLASGRAAGVFKPGNHGSTFGGN
                     PLACAAALETLAVLDDERLLDNAGRMGQALREGFAVALAGLPGVIDIRGAGLMIGIEL
                     DRPCAELVGRALQAGLLINVTADKVIRLLPPLNFSAEEAAALVEGLAPLVKEFLAGQ"
     gene            32277..32570
                     /locus_tag="PKX01_05490"
     CDS             32277..32570
                     /locus_tag="PKX01_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HMZ75610.1"
                     /translation="MIQKLEHFMIVGRTSDGKTFRPSDWADRLCGIMSAFGADNRMTY
                     SPYVRPACNLRGDKTVLVDARIHDIEPLAYNFLKNFAKDNDLQVEVLPDDHFD"
     gene            complement(32667..>34712)
                     /gene="lon"
                     /locus_tag="PKX01_05495"
     CDS             complement(32667..>34712)
                     /gene="lon"
                     /locus_tag="PKX01_05495"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMZ75611.1"
                     /translation="PNNEVEAMRRAIISQFDQYVKLNKKIPPEILSSLSGIEEPGRLA
                     DTIAAHLPLKLEQKQEILEMFNPGERLERLLSQLETELDILQVEKRIRGRVKRQMEKS
                     QREYYLNEQVKAIQKELGEGEDGADLEEMDKKIKASGMSKEAAAKAQGELKKLRLMSP
                     MSAEATVVRNYIETLVGLPWKKKSRISKDLAEAEKVLDQDHYGLEKVKERILEYLAVQ
                     QRVDKLKAPILCLVGPPGVGKTSLGQSVARATNRKFVRMSLGGVRDEAEIRGHRRTYI
                     GSMPGKILQNMTKVGVRNPLFLLDEVDKMGQDFRGDPSSALLEVLDPEQNATFVDHYI
                     EVDYDLSDVMFVATANTLNIPAPLLDRMEVIRLSGYTEDEKVNIAQRYLVPKQMKNNG
                     IKREELSVTEDAIREIVRFYTREAGVRGLEREISKVCRKVVKAIVLGKRKAKVVVNAK
                     NIDKYLGVRKYTFGVAEKQNQIGQVTGLAWTEVGGELLTVEAVALPGKGKIMTTGKLG
                     EVMQESIQAALSVVRRRSKSLGIAADFYQNSDIHIHLPEGAIPKDGPSAGIAICTGLV
                     SVLTGIPVRCDVAMTGEITLRGEVLPIGGLKEKLLAAVRGGITRALIPSENVKDLTEI
                     PDNVKNALEIIPVKWIDEVLELALERRPEPLPDTPAPEVPVAAAADQPADNGGVVKH"
BASE COUNT         6241 a        11360 c        11204 g         5907 t
ORIGIN      
        1 cctgggccag cagcttgtcg aagcgggcgt tggccttctc ctcggcgtcg agctgatcgg
       61 ccttgcggac ctggtagtcg gcgtagcggc cgggaaaact cgacagcagc ccgcgatcca
      121 gttccacgat gcgcgtggcc acgtggtcca ggaagacccg gtcgtgagtg atcaccacca
      181 cggcaccggg aaactcccgg atcagcgctt ccagccacag gatgccgtcg atgtccaggt
      241 ggttggttgg ttcgtccagc agcagcactt ccggttcgcc caccagcgcg cgggccagag
      301 ccacccgctt gatgccgccg ccggagaggc tgccaaccgg cgcgtcaccg gcgagccgca
      361 gcgtgccgag gatctgctcg acgcgttgtt ccaggcgcca ggcctgggcg tcctcgacgc
      421 gatgctggac ggcctccagg tgcgccagcg cagcgtcgct ggcctcgtcg gcgacggcca
      481 gcatggccgc gtggtaatgg accagcaatt gggccacgtc accgaggcct tcggccaccg
      541 ccgcgaaaac gctcaggtcc agggaaaaat cggcttcctg cggcacatag gcgatgcgca
      601 cgccgggctg ccgccagacg cggccatcgt ccagggtggc ctgtccggcc agggccttga
      661 gcaggctgga cttgccgctg ccgttgcggc cgatcagggc gacccgctcg ccggcgtcga
      721 gttgaaagtc ggccttgtcg agcagggcca cgtggccgaa ggccaggcag gcatcgtcca
      781 gagtgaggag cggcatgaat cggtccgcga acgcggtcag ggcagtgacg agaggcggat
      841 tctaccgcgg gcacgcgatg cggcctgtcc gggatacgtc agtcgtgttg gcatgtttga
      901 tgcgtcacac ccctcgatac agcaggtgtt gacggatttg cgacgaatct atttaatagc
      961 aatcattctc attctcgaat cagcgaccga ggtgtgtgca tgagtccagc cctgtccccg
     1021 tcttcccaga ccacgaacga cgacgcgcgc cgcgaatccg catcgccggt caccttgcgc
     1081 agcgccgacc tgctccagga tcggggatgc gtgctcatcg aacaccaagg catggtctac
     1141 gccctgcgtg cgacccgcgc cggaaagctg atcctgacaa aatgatcttc gtcgcccctt
     1201 cgcgccagcc gggcgccttc tccacccggc ccatgtccct cctgggcgtc gccgcattgc
     1261 tcgccaccgg gcttgccggc tccctgggat ccctcttcct ggtcctgcat ttctacgcca
     1321 gctgaatcct ggagcgccct cgccatgtac gtctgtgtct gtcgagcgat caccgaacgc
     1381 catatcaccg aagccgtcaa tgacggcgcg cggcgcctgc gcgatctgcg ccgtgatctg
     1441 ggggtcacgg aagagtgcgg ccgctgtgcc cgctgcgccc atgcctgcct caaggaggcc
     1501 attgatcaaa tcggtccggt ctctatactc tcgggatgtc ggactgaatc aacctattcc
     1561 ctatcccagg aggccgcatg aaaggcgaca agaaagtgct gcagcacctc aacaagcagc
     1621 tcaccatcga actgacggcc atcaaccagt acttcctgca tgcgcgcatg tacaagaact
     1681 gggggctcgg caagctgggc aagcatgagt acgaagaatc catcgaggaa atgaagcatg
     1741 cggacgccct gatcgaacgc attctgttcc tcgaaggcct gcccaacctt caaaacctga
     1801 acaagctgct gatcggcgaa aacgtgccgg agtgtctgca aggggatctg aagctcgaac
     1861 tgggcggcca ggcagagctc aaggaagcca tcgcatactg tgagacggtg cgcgactatg
     1921 tgagccgcga gctcttcgag tccatcctcg aagacaccga ggaacacatc gattacctgg
     1981 aaacccagat tgagctcatc gacaaggtcg gtctgcagaa ttatctgcaa tcccagatgg
     2041 acgtgccgtc ctgagcgagt cggtctcggt agacgccaga aagcccgcgc aagcgggctt
     2101 tttcatgaac acgtagcggc gctgcggcgc atgtccggcg tcggctggct tcagggcgcg
     2161 acgtgcaccg gccgggtgag cgacttctcc tcgcagttca ggccgggtcc ttcccggtcg
     2221 atcacgagaa aatcgctgac ttcatccagg gtgagcagcg ggtgatgcca cacaccaggg
     2281 gcaaaattga caccctgccc gtccgcggcc agaaagaccc gcagtcgggc ctcgtccggc
     2341 gtgtcgcggc cgtgcgccac caccaccaga tacgggcgcc ccgacaaggg aacgaaggcc
     2401 tggctgccca gcggatgacg ttccagcatg ctgacgggaa acggcatcgg tcgcggctgg
     2461 ccccggaaaa tcgagacgat ggcccgcccg tcggcaccgg gctcgatgcg ggccagatcg
     2521 tggaagcgct ccgccgtgcc ttcattgatc aggatccgct ggcgtgccga tgcagcctcg
     2581 atcacctcgc cgaagggcgc gaaggcggcc gacgtgagcg gttccggacg caggacgtcc
     2641 ctttcgtcgc ccatcatcgc gtcacccgcc cccacaggcg caggcgcgac acgccgccgt
     2701 ccggaatgat attgaaacgc acgtgggtga ccggcccgag cccggcgatc ccctcccgga
     2761 agacatgcag ggcatccatg cccaggggct gctccggcag cagcaccggc cagaacatgc
     2821 tctgggtgat gatggactgg tccgttccgc catccatacg ggccgcctgg atcgagcagc
     2881 ggtccggaaa attgcccttg aagtgggcgg tgtccacctc gatccggtcc acaacgccgg
     2941 gggcgcccag ctgcaggatg caccagtcgt tgccgggttc ccggcgacgg cgcgtctccc
     3001 aaccgtcgcc catgttgatg ccgcgcccgg gcgcgatcag attggcagcg gaaccgaagt
     3061 gcgcgtcgtt ccaggccacc gcccgcccac cgttttcgag ggccacgaga tcgtattccc
     3121 gttcgggatc cgacgtgtcg aatacgccca ccggctggcc gtacacccgc agccgggcaa
     3181 tgccgccgtc cggatagatg ttgatgcgca gatggctcca ggtatcggaa gactcgatct
     3241 ccagcaggtg atggctgttg ccggtcaggg aagtggacgg caggatgcgc gtccagcggg
     3301 cgcggacgag caggtccggt tcgtccgatt cgcagtcggt ggcgtcgatc gacactgccg
     3361 gcgcgaaatt accggtgaaa tggctggtat cgatgtcgaa gccgcgcacg atgccccgcc
     3421 gtcccaggcg gacgaggctc cagtcgtaac cagtggtgcg cttgcggcgg gtctcccagc
     3481 cgtccatcca cttcccgttc gcgtcgtact gaccgggcac aaagaccgcg ggctccggat
     3541 tgagcatgcg ctccagcggc gcgaagaatt cgtcggtggc aaacagcgcc cgcgcaccga
     3601 tccggctgtc ggccagattg acggagcggc gcgcccagtc gggcaggacg gccggtgcgg
     3661 cgatgcgcgg cggatcgaag gaatgggtcg tcatgcaggc gctctccagg ggtgatgcgc
     3721 aatccagtgg cgggcaatgt caatgcggcg gcagatccat acgcggtcat gacgcgccag
     3781 gtggtcgagg aatcgttgca gggcgaggaa tcgtccgggg cggcccagca ggcggcagtg
     3841 catgccgatg ctcagcatgg ccggacgctc gtcgccttcg gcatagagga cgtcgaaact
     3901 gtcccgcaga tactggaaga acgggtcgcc gtggctgaag ccctggggca gcgcaaagcg
     3961 catgtcgttg gtgtccaggg tgtagggcac gatcagatgc ggcacacgga cgccatcgct
     4021 gcgggtcacc tgcacccaga agggcagatc gtcaccgtag tagtccgagt cgtagagcag
     4081 gcggccgtcg tcggcgacga ggcggcgggt gttgggcgag tcgcgcccgg tataccagcc
     4141 cagggggcgc gtgcccgtca tgtcctcgat gacgtccagg gcctgggcca tgtgggcacg
     4201 ttcggtggct tcatccacat tctggtaatg gatccagcgc cagccatggc aggcgatctc
     4261 gtggccgagc gccacgaagg cggcggtcag ctccggatgg cgctgcaggg ccatgccgac
     4321 cccgaagaca gtcaggggca gcccccgccg ctcgaattcc cgcaggatgc gccacacgcc
     4381 tacccgcgat ccgtattcgt agatcgactc catcgacagg tggcggtccg gataggcggc
     4441 cggattgaac atttccgaca ggaactgctc cgaaccggga tctccgtgga gcacgcagtt
     4501 ctcgccgccc tcctcgtagt tcagcacgaa ctgcaccgcc acacgggcgc gccccggcca
     4561 gtcggcatgg ggcggatggg cgccatatcc gacgagatcg cgggggtatt cggaattgtg
     4621 tatggggctc atgagggcct cactctatac tgcgccccac attttttcgt gaggatcgaa
     4681 ggatggaacg ctcgcccaat agtccctacc gggacgacat cgtgatctcc tggccggaac
     4741 tgcaccggga cacccggctg ctgtgtcatc agttgatgga aaaaggcccc ttcaagggca
     4801 tcatcgccat cgcccgcggc ggcctgattc cggcggccct ggtcgcacgg gaactggaga
     4861 tccgcctgat cgacacgatc tgcgccgcca gctacgaagc cgacaacgac gacaccgccc
     4921 agaccgtgcg ccgggaggtg aagatcctga aaggcatcga acacgacggc gacggctatc
     4981 tgctcatcga tgacctcgtg gacaccggca atacggcgcg ggtcatccgc gcccagctgc
     5041 ccaaggccta ctttgccacg ctgtacgcca agcccatggg acgccaggtg gtggacctgt
     5101 gtatcaagga gttcagccag gacaaatgga tctatttccc ctgggacatc gactaccagt
     5161 tcgtgccacc catcaagaag catcatcgtc ccgcctgatc aacggtagcc ccccgcggcc
     5221 caggccgcga tccgttcgcg ttcagagtcg gtcatgcccg tgacgttgcc cagcggcatg
     5281 gccttaagct gcacggcctg agcgaggatc cgcgggccat tggcgcggat cgcctcggcg
     5341 ctgtcgagca gcacgccagc cggcgccgcc ggaatcagtg tcggccgggc cgcatggcag
     5401 ccggtgcagc gcgccaccac gatgggctgg atgtcggcca gcgaggccgc cggactgccg
     5461 gccgccggcg ccgcgtgccg gggcgccagt tcgacgaata cggccagcag cagcgcggca
     5521 cccagcgccg gatactgcca ggccacctgg cccttgtggc gcaggttgaa gaagtggcgg
     5581 atcagcacgg ccgccagcag gatcatcgcc aggacccgcc atgcctgggg atggttgtat
     5641 gtgaaggcgt agtgattgga gatcatgcag aacaacaccg gcaaggtcag gtagttgttg
     5701 tgcacgctgc gctgtttggc ccgtttgccg tggatcggat cgggcgtctc gccccgctgc
     5761 atggcggcga cggtggtctt ctggccggga atgatccaga agaagacatt gccgctcatg
     5821 gtggtcgcga tcatggcgcc cacgtggaga aaggccgccc ggccggacag cagatgggtg
     5881 aggccccagg ctacggccac cacgaaagcg aaatacaggg cggcgaacag cagcaggctg
     5941 cgattgatga gcagccggca cagaccgtca tacaccagcc aaccgcccgc cagggtcgcc
     6001 agaccgatgc cgatggccgc cgcaggactc aatgccgcct tggccggatc caccatgtag
     6061 gttgccgcct gggagtaata cagcacacca agcaggaaga agccggtgat ccaggtggaa
     6121 taggactccc atttgaacca atgcagttct tcaggcagat gctgtggcgc cacaaggtat
     6181 ttctgcggat tgtagaagcc gcctccgtgg accgcccaca actcgccacc gacgcccttg
     6241 cggatcaatt ctggatcggt cggtgccttg agggaattgt ccagccacac aaaatagaat
     6301 gaggagccga tccacgcgat cccgacaatc aggtgaagcc agcgcagcaa cagcgacccc
     6361 acatcgatca gataagcggc gtccatcagc ttccccggta ggtggaatag gaccagggcg
     6421 acaccagcag cggcacgtgg taatgccccg ccggatcggc aatgccgaac gccaggggca
     6481 cccggtcgag gaagggcggc tcgggcagtg cgacgccgcg gttccggaaa tactcggcga
     6541 cggcaaaggc cagttcgtac tgtcccggga gcagagtctc cccgctcaac aggggcgctt
     6601 cactgcggcc gtcggaattc gtctgcccgg cagacagcag cgtccggcaa tcgccgtcga
     6661 gacggtataa ctggaagtgc atgccggcgg ccggaacacc ggcggccgtg tcgaggacat
     6721 gtgtggtcaa tcgccccatg ggacagctcc gcgtcgggaa ggaggatggg tcgattataa
     6781 aaagccgcgc aggaagcggg acgtataaag gctatcggac tttccagatg agcgaccgga
     6841 cccggggtgc cgaaacggtg atatatgaaa gcgcgaaaga tctgtattcc gcctgccggc
     6901 ttgatggggt acgggccggc ttcgtaagct tcgggcgtcc gtcatatcaa ggggatcatc
     6961 atgccctcgc gtttcacgcc cgcccagctg tccgacctcg atcgagccga tttcattgcc
     7021 gccatcggtg gtgtcttcga gcattcaccg tgggtcatgg cccgggcgtg ggagatgcgt
     7081 cccttttcaa gccgggcaga cctggatgcc gccctggtga aggtcattcg tgaagccgga
     7141 cgggatgaac tgctcgccct gatcgaggcc cacccggaac tcgccggaaa ggcagcgatc
     7201 cgtggcgacc tgacggccga ttcggcgcgg gaacaggctg gcgccggact caacgcgtgt
     7261 tcgccggagg agttcgagtt gatccagcgc ctcaatcggg cctatcgggc acgctttggc
     7321 tggcccttca tcgtcgccgt gaagggcatg acgcgccacg acatcatcgc ggccattgag
     7381 cgtcgcctgc cccagacctc caccgaggag tttgtcgaag ccctggcgca gatcctccgg
     7441 attgccggct ttcgcctgga cgaactgctg accgacgcct gacgcgtcgg gtcggcccca
     7501 cggggggcag atccgcccgc cgccagccta gaccgtggca ggcgttgcga cctgagcggg
     7561 aacgcgcgtc ccggcagcgg cgggggctcc ggtcttttcg cggatgactt cgatgatctg
     7621 gtcacggaac cagcggcatt cctcggaatg atgggcgcgg tcgtgccaca acagataaaa
     7681 gcgcattttc ggaaaatcga ggggcgactc ggagaagcac aactccccga gggagcagca
     7741 atactccgca aagatgcgtg gcgccgagaa gatgatgtcg ttctgcagca ggacgtaagg
     7801 caccagattg aaatacggca cgctggccac cacgttgcgc ttcagacgct cccgcgccag
     7861 ctgcatgtcg atgacgcccc gctgccccac ggcatagggc gtcggcacca ggtgttcggc
     7921 attcaggtag tcgtcgacgg acagggtgcg cccggccagg ggatgactgc gccgcatcag
     7981 gcacaccaca tcgtcgtcga agagcggcgc caggcgcagg tgttcgggag gttggggcca
     8041 gttgccgatg accgtatcca gctcgccgga ttcaagcgcc ttggcgtagt cgtagtccgg
     8101 cccgaaggaa tgcaggacga cctgggaatt gggtgccagc cggcgcagac gcgccacgat
     8161 gttgccgatg aggatcgcgt tcaggtaatc gggggttgcg atattgaagg tccggcggga
     8221 cttcatcggg tcgaagcgga tctgctggag tccgatggcc tcgatctggg cgagggcgac
     8281 cttgaccggc tccagcagcg ccatgccccg ctccgtgggg gtcatgccgt tgcggctgcg
     8341 caccagcagc tggtccccgg tgatgtcccg caaacggcgc agcgccgtgc tgacggcggg
     8401 ctgcgactga ttgagacgcc gcgccgcatt ggacacgctg cactcggtca gcagtgcgtg
     8461 cagcacctgc aggagatgta cgtcgatgga ttcccaggag caagtcgtgg ccatgatttc
     8521 cctctgaaac acgccgtatg gtgatgttgc agagcaaaaa tcgcgccgat gggtgaatca
     8581 cgggaaaaag ggcatttcca gccgaaaaac ccacattggg cgcccgattg cgcaccggga
     8641 gggcgtgccg gagggcctga cggcaccatt tcgatgcctg ccacgcaccg accggtcgga
     8701 tcgtcacgct ggatcccgtg gcccatgaaa agacgccgcc ggcgtggtgc cggcggcgtc
     8761 ctgggagtgc tgcgggcgaa ggctcagaag tggtattcgg cgcggagcat cggcgtcttg
     8821 gcaaaggcac cgttgcccgc cgcgccagtg tggtcgttgc caaacttgtt gcgccagtac
     8881 tggtattcga cgccgacctt gaaggtcttc gggcgcgcac cgaccaaggg gctcgcgtca
     8941 tacatgatct ggccgtcgaa gttggtttcc ggcttcgtcg ggccgccgaa ctcgtttgcg
     9001 cccttggcgg cgatgtagtt catgaagcct tcaaaggtca gcggcaggga gcccagggga
     9061 atcccccagg cgagggtcag catgggatgg gcgtcgtagc tgtagcggct cggatgggtc
     9121 cgcggcgcat tgctttccca gagctgcagc acgctcaggt tgaggaagcc gggcacatcg
     9181 aacatcacgg tcgggccagc caggggcatg cgcttgcgcg actggtagct gtcgccggac
     9241 ttggtgttgt aatcgaaacc gacggtgaat cccaggccgc gcacgatgcc gaacttgaag
     9301 gcgtcgggat tgccggcgat cttgccgaaa tcgaaggtgt gacgatagac cacataggtt
     9361 tcctgggccc cgccaccgtt gttgaaggaa tccttgctgt cggaaaggag catgtccaca
     9421 ttgaggaagt tcgtgccgta cttgtagccg ctcacatgtc cgagattgaa gatgttcttc
     9481 ttgatgtcgt caggattgta cggttcggcg aacttcgtcc cgtagcgcca gccgacatag
     9541 gtgtcgctcc agtcggcagc gtgggcacag gagaggccgg caatcagcat tgcgcagccc
     9601 agggcggttt tcttgaccag catggatgga cctccggagg tgttttcaag ggggctcccg
     9661 cgctgttttt gcgacgcagc aacgagacgt tggtcaaact gtaacaggaa gttttccacg
     9721 aaggccgaat acccgatatc cggaaatgtg tatatcccgg tcgttatacg tgcctgaata
     9781 taatggcctt attgtgtccg atatacccgt atagcatgca tcaacttgag gcattcgaac
     9841 ggttttcgca ccgttttgag gcgcgatttg cggcgatttc cggggtcacc cccggtcacg
     9901 cctcgcgcga tccgggtgag tcttttcccg aaaatatttt ccacttataa accaatgtgt
     9961 taccacctgg catgacgatt gctgaagctc cgctcattca accgtccagg agccctgtcc
    10021 atgcctatcc ccttcaatgc cggccgccgt ctggtgctgg ctgccgccat gtccctgctg
    10081 gccatgcccg catttgccga cgccccgacg cggatcaagt tcaccctcga ctggcgcttt
    10141 gaaggaccgg ctgcgccctt cctgttggcc aaggccaaag gctatttcgc ggccgagggc
    10201 gtggacatgg aaatcgactc cggatccggc tcggcgggcg cggtcacgcg ggtggcgacc
    10261 ggcgcgtacg aaatgggctt cgccgacttc aacgccctcg tcgaatacga tgccaagacc
    10321 ccgggctcca agatccaggg tgtttacatg gtgtacaacg ccacgccggc tgccgtattc
    10381 gtgctcaaga agtcgggcat caccaagcct gccgatctgg ctggcaagac cctcgctgcg
    10441 ccgatcttcg acgcgggccg caaggcctgg ccggcctttg ccaaatccaa caagctggct
    10501 gtggatgccg tcaagtggca gaccgtggag ccggccctgc gggaaaccct gctcgcccgc
    10561 ggtgacgtgg acggaatcac cggcttctac ttcaccagcc ttctcaacct ggaggcccgc
    10621 ggcgtcaaga gcgacgacat cgtggcgctc aaatacccgg acttcggcgt tgagctgtat
    10681 ggcaacgcca tcatcgccag tcccaagctg atggccgaga atcccaaggc ggttgccggg
    10741 gtgctgcgcg ccttcaacaa ggcactcaag gaagtcatcg ccaacccggc cgcggcgatt
    10801 gcctacgtca aggaacgcga tccgctcatc aatgcggacc tggaaacccg ccgcctcaag
    10861 ctggcgctcg actccagtgt cgtcacgccg gaggtcaagc tcaatggact gggtgccgtg
    10921 acgcctgagc gcatgcaacg ctccatcgcc gagaccgccg aagcctacgc gctgcccaag
    10981 gtgccgacag ccgccgatct gttcaatccg aacttcctgc cgtccaagac ggaccgcacg
    11041 ctcaagtaag ccctgcgccg cgggacgggc gcacgtgccc cgtccctccc ctcctccacc
    11101 ggaactcccc atgtcgttcg tcacctttga agacgtcagc ctggcctata cgccgggcgg
    11161 cgcaaatgcc atcgagaacg tgagcctgag catccgcgag ggcgagttcg tcgccgttgt
    11221 cggcccttcc ggctgcggca aatccaccct catgaagctg gtgtcgggcc tcaagccgcc
    11281 cagcgccggt tacgtctatg tggatggccg ggaagtcggc ggccccctga agatcgtcgg
    11341 catggccttc caggcagcca acatgctgcc ctggcgcacc acgctggaca acgtgatgct
    11401 gcccctcgag atcgttgagc cttaccgcag ccgcctgcgt cgcgagcggg agaccttcaa
    11461 ggctgacgcg gaagccctgc tggcacgggt gggcctggcc ggtttcggtc agaaatatcc
    11521 ctgggaactc tcgggcggaa tgcagcaacg tgcctccatc tgccgggcgc tgatccatca
    11581 gccccgtctc ctgatgctcg acgaaccctt cggcgcgctc gatgccttca cccgcgaaga
    11641 gctgtggtgc atgttgcgtg atctgtggca acagcagccc ttcacggtgg tgctggtgac
    11701 ccacgatctg cgggaggcgg tgttccttgc ggacaccgtc tacgtgatga gcaaacgccc
    11761 cggccgcatc ctgatccgca aggagatcga tctgccccgc ccgcgtccgc tggacgtcac
    11821 ctacaccgac gcctttgccg gctacgtgca cgagctgcgc gagcatatcg gccgcattcg
    11881 cgaatcctga ggagcacccc catgtccctg aagcaatcca ccctcgtgaa gagcgctccc
    11941 tggctcctga ccgccgtgct gttcatcgtc tgggaactga tctgtgtcgg ctttgcgatc
    12001 cctgactacc tgctgccggc ccccacggcg atctgggcct ccggcctgga gaacgccgcc
    12061 cccatcatga tgcacgccac ccagaccctg atgaccaccc tggccgggtt cgccctggcg
    12121 gtggtcgtcg gcatggtcct gggcctggcg gtcggcgcct cgccggtggt gtacaaggcc
    12181 acgtacccgc tgctggtcgg attcaacagc atccccaagg tggccttcgt gcccgtgctg
    12241 gtggtgtggt tcggcatcgg caccatcccg gcgatcctca cggccttcct gatctccttc
    12301 ttcccggtgg tggtgaacgt ggccacgggc ctggccaccc tggagccgga gctggaggac
    12361 gtgttgcgct ccctcggcgc ccatcgctgg gacatcatgg tcaaggtggg actgccccgc
    12421 tccctgccct acttcttcgc gtccctgaag gtggccatca cgctcgcttt cgtcggttcc
    12481 gtgatctccg agaccgtcgc gtcgaatctg ggaatcggtt acctgatgat gtccgccagc
    12541 tcatcaatga acatgcccct ggtgtttgcc gggctgatcg tgatcggtgt gatgggcgtg
    12601 gtgatgtacg agctgtttgc gctgcttgag cgcaacctca ccggctgggc ccatcgcacg
    12661 cccggcgcag ccacctgaac cctaaggcgc cgcactgggt ggaagggtga aagaggctcg
    12721 aaggggcctc ttgctccacc accggacgga tgattcatgc accggtccat accgaccggc
    12781 cccctctcag cgctggaacg caggcgcgcc gagcacgtgt gtggccacga cacagcgttc
    12841 gtcgccgagc accgtcaggg caaagaggcg ctccgccagc cccgtgacac cgcgcaggcg
    12901 gaactccagt tccggcgtcg cgcgctcgtc gagcaccacg cagtcggcct ccttgccgac
    12961 ctcgaaattc ccgatgaagg catccaggta caaggctcgg gcacccgcca gagtggcgag
    13021 ataccaggcg cgcaggggcg acaggggacg ccggtggacc tgggagacct tgtaggcctc
    13081 gtcgagggta cggatcaggc tgaaggaact gccgccgccc acgtccgtcg ccagcccgac
    13141 ccgcagcccg gcctccaccg acgctgcgtg gtcgaagaaa ccgctgccca gaaacaggtt
    13201 cgacgtcggg cagaaggccg ccgccgtgcc ggtcgccgcc atctcggcac gctccgcccc
    13261 ggacagatgg atgcagtgac catagatcgt gcgcggaccg agcagaccat ggcgcgcata
    13321 cacgtcgaga tagtcgcggc actcgggaaa caaggccttg acccaggcga tctcgtccgg
    13381 attctcggcc agatgggact gcacatgcag atcgggccgg ctcgcgtaaa gttcgccggc
    13441 cagggccagc tgggccgatg tcgaggtggg cgcaaaacgc ggggtgatcg tgtaacgcag
    13501 gcgcccgcgc ccatgccagc gctcgatcag ggccacacag tcccgctcgg cgtcggcaac
    13561 ggtgtcgcac aattcgggcg gtgcatgacg gtccatcatc accttgcccg tcagcatgcg
    13621 cagttggcgc gcgtccgctt cggccatgaa ggcctccacc gacgcggcgt gcaccgtggc
    13681 aaacaccgac gcggtcgtgg tgccgtgcgc cagcaggcgg tcgaggaaat accgtgccgt
    13741 ggcgcgcgcc acctccgggt cgcggaaggc catctcggcc gggaaggtat aggtattcag
    13801 ccagtccagg agctgccggc caaaggaggc gatcacggcc gactggggat agtggcagtg
    13861 cgtatccacc agtcccggca ggaccagccg atcgccatag tcgaaacgtt cggcggcggc
    13921 caggcgttcc ggcgcgatcg cagactccac ggcacggcgg tgcccgacgg cagccacgcg
    13981 gccctccacg atccacaggg cgccctgggg aaaatgggcg tagctgtggg ggtcgtcccc
    14041 ttcgccggga tcgtcgagga aatgcaggat ctgcccgagg atcaggcggt ccggaacggt
    14101 ccgatgcgtc atggcggcct ctcaggcgcg atggtcggcc agctggcggg ccaggcggtt
    14161 gtggtgggcg atcagtggcg gcagttccag gggcacgagc tgaccttccc ggaccaccgg
    14221 ctggccattg atcaccgaca ccttgacttt cggcggcgtg cagaagacca gggcagccac
    14281 cggatcgtga accgcggcgc cggccagatc aagttcgtcg gtgggaaaag ccaccacgtc
    14341 gccgctcatg cccggcgcca gggcgccgat atcgtcgcgc cccagcaccc gcgcgccgcc
    14401 gagggtggcg agctccaggg cctgccgggc gctcatggcc gacggtccgt agccgacccg
    14461 cgccagcagc atcgcctgac gggtctcgcc gagcagatgg gccgcgtcgt tggacgcgca
    14521 gccgtcgacg cccaggccca ccggtacgcc cgcggcgcgc atcgccggaa tcggcgcgat
    14581 gcccgaggcg aggcgcatgt tggaacaggg gcaatgggcc acgccggtgc cggtgcgggc
    14641 aaagagttcg atccccggtc cgtccagctt gacacagtgg gcatgccaca cgtcggcgcc
    14701 aacccagccg agggattccg catattcggc aggcgacatg ccgaattttt cccggctgta
    14761 ggccacgtcg ttgtcgttct cggccaggtg ggtatgcagc gagacgccgt ggccgcgggc
    14821 caggatggcg gcctcacgca tgagttcgcg gcttaccgag aatggcgaac agggcgccac
    14881 cacgatacgc cgcatggcgt gccgtgcggg atcgtgccag gtctcgatga gacgctgagt
    14941 gtccttcagg atggccgcct cgtcctccac cagccggtct ggcggcaggc cgccggcgct
    15001 ttcgccgaca ctcatgctgc ctcgcgcggc atggaagcgc atacccagga tcgcggcagc
    15061 ctcgatggcg tcgtcgaggc gactgccgtt gggatagagg tacaggtggt cgctggaggt
    15121 ggtgcacccg gagaggatca gctccgccat ggccgtgagc gtggacaccc ggatcatctc
    15181 cggcgtgaga cccgcccaca ggggatacag ccgccgcagc cagccgaaca gttctgcgtc
    15241 ctgggcaccg ggaatggccc gtgtcaggct ctggtacatg tggtggtgcg tattcaccag
    15301 gcccggcagc gctacgcacc cggcaaggga gatcacctcg tcggccccgt cgggaagctg
    15361 gtcggacggt ccgaccgcct ctaccacgcc atcgcggatg aagacgccgc ccccgggaat
    15421 ttcccggcgg gtggcgtcct gggtgacgag gacttcggcg tccttcagca gcagggtggc
    15481 catcgcggtc agggcaggaa ggatttgccg agggaggccg gcaggttgag ctgaagcagg
    15541 cgccggtccc gggagatgaa caccagcacg atcacggtcg ccgcgtacgg caaggcggcg
    15601 aggagctgca ccggcacgtc cagaccaagc ccctgggcgt ggaactggag gatcgtcacc
    15661 ccgccgaaca ggtaggcacc gaggagcaga cgcagcggct tccaggtggc gaacaccacc
    15721 agcgcaacgg cgatccaacc ccgcccggcg ctcatgttct gcacccacag gggcgtatag
    15781 acggtggaca gataggcgcc cgccagaccc gccatggcgc cgccgaagac caccgcgcca
    15841 taacggatcg cgatcaccgg gtagcccacc gcgtgggccg cttccgggga ttcaccgacg
    15901 gccttgagaa gcagtccaag ccgggtcctg gcgaggatcc aggccacgcc ggcgaaggcc
    15961 gcaaaggaca ggtacaccac tgcatcctga tgaaacagga ctggcccgag caccggaatg
    16021 tcggcgagca acggaatgtc caccggcgca aggcccggca ggctgtagct cacgtaatgc
    16081 tgcccgatga aggccgacag gccgatgccg aagatggtca acgccagccc gcaggccgcc
    16141 tggttggcag ccagggacaa ggcaaacacg gcgaacagca gcgccgtcgc catgccggcc
    16201 agcgcaccgg ccggcaagcc cgccgccgca ccgccaccgc tctccgccgc cgcggcgaag
    16261 ccagcgatcg ccccggtgag catcatgccc tcaaccccca ggttgatcac gccggtgcgc
    16321 tcggccacca gttcgcccag ccccgcaaag atcagcggcg tggcggccgc gatggcgccg
    16381 gccagtacag gcacaagatg ttcgatcatg gcggattacc tcgaaggacg gcgcagacgg
    16441 ttgtcgatga aggcatcggc ccccagcagg aaaaacagga gcatgccctg gaagatgccg
    16501 gtgatggccg ccggcatctg cagactcacc tgggccgcct cgccgcccag gtagatcagg
    16561 gccatcaggg cgccggcgag cacgatcccg agcggatgaa gtcgccccag gtaggcaacg
    16621 atgatggcgg cgtacccgta gcccggcgag atgttgaggt tgagctgacc gaccgggccc
    16681 gccacctcac ccacgcccgc cagcccggcc gtgaggcctg acatcgccag gctgaaccac
    16741 accgtctggc tggcggaaaa gccggcatag cgggctgccg cgggcgcctg gccgcccact
    16801 tccagctggt aggccaggaa gctgcgggca ttgaacagcc agaacgccag caccgcaacg
    16861 agcgtgatga acaccgagct gttgacccgc gagccttcca gcaggatgcc gaacagggcg
    16921 ttgtcatcga acatgatgga ctgggggaag ttcatcccct ccgggtcccg ccaggggccg
    16981 ttcaccatgt aagacaggat gaaggtcgcc acgtaggtga gcatcagcgt ggtcagggtt
    17041 tcttccgcgt tgaagcgggt cttcagcagc gccggcaacg cgccccacag ggccccgccg
    17101 atggcgcccg ccaccaccat ggccggaatc acccacgcgg aggtgctgcc gttgaagtgg
    17161 atcgcgaccc ccccgccaaa cagcgccccg acgatcagct ggccctcggc tccgatgttg
    17221 tagatgcgcg ccttgaagcc gatggcgagc ccctgggcga tgaggatcag cggacaggcc
    17281 ttgatcagca gctcgctcca tccgtagctg ctggtcaggg gctggacgaa gaagacctcg
    17341 aaggatgtga gcggtgattt gtccagggcc cagaagagca gcgaaccggt cagcagggtg
    17401 gcagcgatgg caatgagggg cgccgtccag cgcatgagct gggacggttg ggcacggggt
    17461 tcaagcagat gcatggacgg attctccgga ttgggcggcg gtgaacagtc cggacatctg
    17521 cagtccgacc ttctccgcgt tggtcgcggc cttgggctcc ggttcggcga gcttgccggc
    17581 cgccagcacc gcgatgcgat cgctgatctc gaagagctcc tccagctctt ccgacaccac
    17641 caggatggcc acgccggtgc gtgacaggtc cagcagggtc tggcgcagga aggccgcggc
    17701 ccccacgtcc acgccccagg tgggctgggc gacgatcatc accttcggcg acagcatgat
    17761 ctcgcgcccg acgatgaact tctggagatt accgccggac agggagcggg ccgccgaggc
    17821 ggcgccaccg caacgcacgt cgaagcgggc gatgcactgc tcggcgaagg cgcgggcctt
    17881 ggcgtaactg accaggccca ggcgcttcat gccctgccgg tgggccgtga gcagcgcgtt
    17941 gcgggccagc gacagggacg gcaccgcacc ccgtccgagg cgctcttccg gaacaaagac
    18001 gagcccccga tcccgccggc gacccgcgtc gagatgcccg atcgccgcac cctccaagca
    18061 aatcgcgtcg cgcgccaccg gcgtctcgcc ggacagggct gccagcaact cggcctgtcc
    18121 gttgccggag atgccggcga tcccgaggat ctcgcctgcg tggacggtca ggctgatgtc
    18181 cttcaaagac gtgccgaacg gatcgtccga cggcaggctg agaccgctta cctgcagcac
    18241 cgggcggctt tcgccgatga agggcggcgc ctcgcagacc ggcagctcgc gaccgatcat
    18301 catgcgcgcc aggctggcgg gcgtctcgtc ccgcggcgtc gcgttgcccg tcaccttgcc
    18361 gccgcgcatg atcgtcgcgg aatcgcacaa ggccttgatc tcgtggagct tgtggctgat
    18421 atagagaatc gagacgcctt ccgccgccag cgtgcgcagg gtgtcgaaga gcttgagcac
    18481 ggcctggggc gtgaggaccg aggtcggctc gtcgaggatc agcagcttgg gatcctgcag
    18541 caggcagcgg acgatctcca cgcgctggcg ttcgcccacc gacaggctat gcaccagccg
    18601 gaaggggtcg aggggcaggc cgtagcgctc cgacacctcg atcacgcgct tcgccagcgc
    18661 cggcagatcg aaggcttcgt cgaggaccag ggcgatgttc tccaccacgg tcagggtctc
    18721 gaacagggag aagtgctgaa acaccatgcc gatgcccagc ttgcgggcct gcgccgggtt
    18781 ggcgatggcc acctggtgcc cgttccacat cacctcgcct tcgtcggcct tgaccgcacc
    18841 gtagatgatc ttcatcaggg tgctcttgcc ggcaccgttc tcacccagga tggcgtggat
    18901 ctcaccgggc attacggtga ggctgatgtc ggaattggcc accacgctgg gatagcgctt
    18961 ggtgatgtgg agcagttgaa gtcggggttc gggcgtgctc atgcttactc ccgctggacg
    19021 gggttgtcga tcaggcgggg gcgggccgcg tcgcccacaa gtcggcgggc ttcgtgaacc
    19081 tggaaaattt cggccgcgac ggccagggca atggcggcag gcgccttgtc gacgatgccg
    19141 gcgatcccga tcgggcagac cagttcatcg aggcgttccg gattcaggcc tcgatccacc
    19201 aggcggtgct cgaaggatct acgcttgcta cgggagccga tcagcccgaa ataggcgaaa
    19261 tcccgccgcc ggaagatggt ctcgcacagg ctgaaatcga gggcgtggct gtgggtcatc
    19321 accacgaaaa agctgccggc gggtgctctc gccacctcgc tttccggcac gtcggtcagc
    19381 accgtgcgga catccgtcac gtccagaccg tcgaaggcgc cgtcacgggt gtcgacccag
    19441 gtgatctgcg ccccgagcag ctgcagctgg cgcaccagcg cctgggccac atgacccgca
    19501 ccgaaaagct ggatcgggaa cgccagaccg ccgacatgct gggcgaatcg ccagtctgcc
    19561 gccgtgcccc cgagccgcgt ctcgccacct gccacgggcg cccgctgcca gaccgaagcg
    19621 gcggcctccg aatccacctg gcgatgcagg tgatggcctg ccagcaccga cgcgcggcgc
    19681 tccacccacg catcccgctc ggacgccggc atcgactcga acaccagcca caccaccccg
    19741 ccgcagcact gacccaaacg tgcgcccagg ttgtagcgga cgatttccgg acggccgccg
    19801 cccacggcga ggcgccgccg ggcatgactg atggcctccc attccagatg ccccccgccg
    19861 atcgtgtcca cgatcgtctc gcgacccacc agcatggccg cccccgcctc gcggggggtc
    19921 gatccatccg cccgggcaac cgtcacgagt tccacgctct gccccgcttc caggacagcg
    19981 ggcaaggcct tcagccagtc gggcgcgcgg gtcgtcatgc gccatcctcc ggcgccaggc
    20041 agcccagcgc ccgcaggacg gcttccggcg tggccggcgc ctccagccgg ggtgattgcc
    20101 ccgccggcag ggcggccgcc acggcatcgc gcagggccat gaagaccgac agggccagca
    20161 tgaagggcgg ttcgccgaca gccttggagc ggtggatgct gtcttccacg ttggcattgt
    20221 cgaagagccg cacgttgaac acgtccggta gatcggtggc agtcgggatc ttgtaggtgg
    20281 acggcgcgtg gctcatgagc cgcccggcct gggggccgtc cggtcgccac cacagttctt
    20341 cgctggtgag ccagcccatg ccctggatga aaccgccttc aatctggccg atgtccaggg
    20401 caggattgat ggagcggcct gcgtcgtgca ggacatccac ccgcaggacg cggcattccc
    20461 ccgtcagggt atcgatggcc acttcgctcg ccgcggcgcc gtaggcaaag taataaaacg
    20521 gccgccccag cttggtctgc gggtcgaagt ggatcttcgg cgtacggtag aagcccgcat
    20581 cccacaactg gatgcgggcc cggtaggcgc gcatgaccag tgccgcgaag ctgtcgtcga
    20641 acagcggtgc ggaggcctca cccgcctgca cgcgcgcatc gacaaagcgc acctgctcca
    20701 ctgccacggc cccctgaacg agttcgacga cgaaggccgc gaggcgtgcc ttgatgtgct
    20761 ggcaggcggc ctgggcggcc ttgccattga gatccgtgcc gctcgatgct gccgtggcgg
    20821 aggtgttggc gacccggctc gtgtcggtgg acgacagacg cacatcctcc agcggcagcc
    20881 cgaactcgtg agcgacgatc tggcgcacct tggtgtacag gccctgcccc atctccgtac
    20941 cgccgtggga gacgagcacc gtgccgtcgg tatagacatg caccagcgca cccgcctggt
    21001 tgtaatgggt ggcgttgaag gagatgccga acttgaccgg cgtcagggcg agaccgcgct
    21061 tgatgactgg actgctggca ttgaaggcga gaatcgccgc gcggcgggcc ccatagtcgg
    21121 acgcatcggc cagttgcgcg accagcgctg gcgccacgtt gtcctccacg gtcatgccat
    21181 agggcgttgt gctgcgggcg ccgggcacga cgtcgtagaa gttcgcctcc cgcaccgcca
    21241 gggcgtccag gcccagatgc cgggcaatgt catcgaggac cgtctcgatg acgaacatgc
    21301 cctgggggcc gccgaagccg cggaatgccg tattggagac ccggttggtc ttgcagcgca
    21361 ggctgcgcag ggccaccgca tccagataat aggcgttgtc aatgtggcag atcgtacggt
    21421 cgttgaccgg ccccgacaga tcggcggaat acccgcagtc ggaggccatc tcgagcctga
    21481 gcccgaggat ccggccggcg tcgtcgaaag ccgactccca gcggtattga tagggatggc
    21541 gcttgcccgt ggcgaccatg tcgtcgtccc ggtccaggcg cagcttgacc gggtgacccg
    21601 tcttcaccgc gagcagggcc gccaggcagg cccactgggc cgactgagat tccttgccgc
    21661 caaagccacc gcccatccgc cggcattcga cgctgacctg gtgcgagcgc cagcccaggg
    21721 cgtgggccac catctgctgc atctcggtgg gatgctgcgt ggagcagaac acatggatgg
    21781 tgtcgctctc ccgcggcacg gcacaggaaa tctggccttc cagataaaag tgttcctgcc
    21841 caccgaggcg cgcagcaccg gacagccgat gcggggcgtt ttcgaaggcc tgttcgaccg
    21901 cgccgcgcgt catcacgaac ggcggcagca cccaggattc ggccgcgatg gcgctgtcca
    21961 tgtcgagaat ggggggctcc gcggcgtact ccacccgcgc cagacgcgcc gcctggcgcg
    22021 ccgcctcgcg ggtctcggcc gccaccacga agatcacctg cccgtaaaac tcgacaaggt
    22081 cgccagtgat gatcggatcg tcgtgcagga tcggtccgca gttgttctcg cccgggatgt
    22141 cggcggccgt cagcacgcag cgcacgccgg gatacgcccg cacggcgctc agatccatgg
    22201 cacggatgcg gccgcgggca caggtggaca ggccgggggc cgcgtgcagg gtgcccgcca
    22261 tttccggcag gtcatcgatg tactgcgcct tgccgctgac gtgcagcggg gcgctttcat
    22321 ggggaaccga gcgaccgacc ggggtcgcgc cggagggaga catccgcgcg ttcatgagcg
    22381 ttcctccggc tggatcgggc ccagcgcaga cagccgcgca ggcagaccgt cgggcggcgc
    22441 cgtcgtctcc agccagaagc gctgcaacag attcgccgcg accgacaggc gataggcggc
    22501 gctcgcgcgc acgtccgtga ggggcgcgaa gtcttcgcgc aaggcggcca tcgccagccg
    22561 aaccgtatcc ggcgtccacg ggcgccccag cagggcggct tcaccggcat cggcccgctt
    22621 gggcgtgcca gccacaccgc cgaaggccag acgcgcgccc cgcacgatgt gctcgtcgtc
    22681 cagatgcacg gcaaagccgc aggccactgc ggagatgtcc tgatcgaagc gcttcgccac
    22741 cttgtacgcc ctgaactgcc ggttcagggg tggcagcggc gaatccagca ccagcgcacg
    22801 gatgaattcc ccgcgctgga gagcggtctt ctgataatcg agatagaacg cttcgagggg
    22861 catctcccgc gtgtgcgcgc cctgttgcag caccacccgg cagccgaggg cgatcagcgc
    22921 gggcatggag tcgccgatgg gcgaaccatt ggccacgttg ccacccagcg tgccggcatt
    22981 cttgaccggc gtggaggcga aacgccgggc cagttcatcc caatgcggct caaaggcggt
    23041 gagcgccgcg aaggcgtcgg taagggacac accggcgccg atctcgagct gcccggcacc
    23101 gacccggatg cgcttgagct ccgccacgcc ccccaactgg atcacgtctc ccagatcccg
    23161 gccctgcttg gtcacccaca ggcccacgtc ggttccgccg gccaccagtc gggcgtccgg
    23221 cagacgctcg cgcagctcgg cgagctcggt cagtgtccgt ggcccgtagc tgtgccgccc
    23281 ggccgccgca taatcgagcg gcggcaattc ggccagcgac ttcagggccg cgacgatagg
    23341 cgcccgctcc aggctccgac gcggcgccag aaaggcgtcg cgggccgcat ccacgatcgg
    23401 ccgatagccg gtgcaccggc atagattccc cgacaggcag tggtcgatct cggcccgcgg
    23461 cggacagatc ggggtgtttt cgtacagtgc gtacagggac atgacgaaac ccggcgtgca
    23521 gaacccgcac tgggacccat gccgctccac gagggcctgc tggaccgggt gcaggtgccc
    23581 gtctgcgctc agatcctcga ccgtgaacaa ggccttcccg tccagggccg gcaggaactg
    23641 gatgcaggca ttgacggcac gcaggcgcag ttcgtcgcct tccggcccct tcgccagttc
    23701 gccgatcacc accgtgcacg cgccgcaatc gccttcggca catccctcct tggtgccggt
    23761 ctggtgcaga tcttcgcgca ggaactgaag cacggtccgc gtcggtgccg gtccctgaac
    23821 ggactgcacg gcgccgcgga aatagaactg gatcgggcgt atgggcatgg cgagctcaca
    23881 acggatgatg cggtgcgaca aaccttagca caggcttttc cgggtcgttg agaggggacg
    23941 acggataagg gttatgtgcc gaagcgtatg gctgctggcg ggacctattc gcgcgtccag
    24001 aagcccgcgt ggggcgccgc agcctcgtct tccaggcagg gtccggtgac ggcgatacgg
    24061 cgctgtcccc gggcaaacac ctcgcggcat ggcagcgaga acgtgggatt ctccgggtga
    24121 tcgccggtga ggccgagcag gcgatgttcc gacagggcgt acaccacccg tccgataccg
    24181 caccagtaga tggcgccggc acacatggcg cagggctcgg cgctggtata gagggtcgcc
    24241 cggctcagtt gcccgggcgt caggcggcgc gccgcaagcc ccgccgcgac cagctccgca
    24301 tgccgcgtcg gatcgccatc gggcggcacg gaatcgttac cggcctcggc aacgatccgc
    24361 ccgagttcat ccacgacgat ggcggcaaac ggatgcttgc ccccatcacg cacgcggcgc
    24421 gacagttcga tgcagtggcg caggaaggtt tcgtcggcgg ctgtcaggat gtcggacatg
    24481 gcggtctccg gaaatggtga cgattatccc ggtacgcacc gggccgacat gtcagtgacg
    24541 ggcaatactc ttcataacgc cggcgagata ggtgcgccgg gcatggattg gtagagtggc
    24601 gcaacatccc gttttctgac caccttagga gatccaagca tgttgctgcg cagactcaag
    24661 tccttcacag cggttgcggt catggcgctg gcgggcctgc acgcggccca cgcggccgaa
    24721 ccgatcaaag ccggcttcgt ctatatcggc cccaccggcg atcacggctg gacctactct
    24781 cacgatgaag gccggaagct gctggaagcc cagtccaacg gcagcgtgaa gaccaccttc
    24841 gtcgagaacg tgcccgaaac cgccgacgcc gaacgggtct tccgggatct ggcgcaaaag
    24901 ggcaacaagg tcgtcttcgg gacctccttc ggttacatga accagatggc caaggtcgcc
    24961 aaggccttcc cgaacaccat cttcatgcac gccaccggat acaagaccgc ccccaatctg
    25021 ggtgtctacg acgtgcggac ctacgaaggc gcctacatgc tgggcgtggt ggccggcaag
    25081 atgagcaagg gcaacaagct cggcgtcgtc gcctccattc ccatccccga agtcatccgc
    25141 aacatcaatg ccttcactct cggcgcccgc agcgtcaacc cggccatcac cacgcgggcg
    25201 atctgggtga acagctggtt cgacccgggc aaggagcggg aagccgcgct ggccctgatc
    25261 gcccagggct gtgacgtgct gatgcagaac accgactcgc ctgccgtcgt ccaggccgcc
    25321 caggaaaagg gcgtgctggc ctttggctgg gattcggaca tgaccaagtt cggcggcaag
    25381 gcccatctgg ccgcgtccgt gctgaactgg ggcgtgatct acaagaagac cgtcgatgaa
    25441 gtcgctgccg gcacctggaa gagcggtgac atctggtggg gcgtgaagga aggcgcggtg
    25501 aatatcgaca actttggccc ggcggtccct gcaccggtca agaagcttgc cgaagaacgg
    25561 cgcgatgcca tcaaggccgg caagcttcac cccttcaccg gcccgctcaa ggagcagtcc
    25621 ggcaaggagt tccttgccgc cggcaagatc tttggcgacg gagatctgaa gaagatgaat
    25681 ttctacgtgg aaggcgtcga aggctccatc ccgaagtaag cagcaccccg gcttgaacgc
    25741 gacctgcggg tcgcgttttt cattgctgcg acgcattgcc gatcgaccgg tttatgctcg
    25801 gcgtacgacc atccctgaag aggtaaaaat gccccctccc ctcgctgccg atgccgcgct
    25861 cgacttcgcc tgccgcatgc ccaaggccga gctgcacgtc catatcgaag gcacgctgga
    25921 gcccgaactg gcctttgccc tggcccgtcg aaacggcatc gccctgccct atgcggacga
    25981 ggacgccctg cgcaaggcct acgcattcga cagcctgcag tcctttctcg atctgtacta
    26041 cgcgtgcgcg gatgtcctgc gcacgcccga cgatttccgc gatctgatgc tggcctacct
    26101 ggagcgggcc gttgccgaca acatcgtcta caccgagatt tttttcgatc cgcagaccca
    26161 tacggcccgt ggcattccct tcgaaaccgc cctgaggggt ctcgaagacg gggtgtatca
    26221 cgcccgccgc ctgtggggca tcgaagggcg cctgatcctg tgtttcctgc gtcacctgag
    26281 cgaggaggac gcatttgcca ccctggcaga agccgagcct ttcctggagc gcattcacgg
    26341 gttcggactc gattcgtccg agcgcggtca tcctccagcc aaattcgccc gcgtcttcgc
    26401 ccgctgtcgc gcgctgggca agccgatcgt cgcgcacgca ggtgaggaag gcccgcccgc
    26461 ctacatcagc gaagcactcg acctgctggg ggcccgacgc atcgatcacg gcgtccgcgc
    26521 cgtcgaggat gccgccgtcg tcgaccgcct gatccgggac gcgatcccgc tcacggtctg
    26581 tcccctctcc aacctgcgcc tgtgcgtgtt tccgagcatg caggaccaca ccctgggccg
    26641 cctcgtcgcc gccggtgcct gcgtcaccct caatagcgac gatccggcgt atttcggagg
    26701 ctacctcaac gacaacatcc gcgccgtgca cgcggccttc gcgttcgata tacccacctg
    26761 gtatcaactg gcccgcaaca gcttccaggc cagctttgca agtccgaccg aaaagcagca
    26821 gtggatcgac cgcctggatg ccgcccgcgc agccactggt ctctgattcc tcgccatgca
    26881 ggcgcaggaa caaatcgccc gacccgtcgc gccttggcag atgaatccga tctgcgctga
    26941 cgtaagcgac tgcggcagga agcccaaggg cagttattga gaacggccac ccgccagccc
    27001 acccagtaag tgccttgaga acgtcctcgt gcccgaacac ccgtgaagtc acgacgaacg
    27061 tcgcctcgca tgcaaaaagc ccgtgaaaac gggcttttta gcgtactgca tataccagtg
    27121 aaacaacccg gcaggtcgcc tggtcccccc gacaggaatc gaacctgtat ctagcgctta
    27181 ggaggcgctc gttctatcca ttgaactaca gggagtcgaa ggggcgcatt ctagcgtgtt
    27241 gcgggcactt cacgccacgt tggcgtggcg cagaccgggg cgcggaccgt cagtgcaatc
    27301 aggcaaagac gtccacattc cgcccgaccg tcgcagtcgg atccggagcc tttgctggct
    27361 gagggagcgc ctggagcagt tgctgggcgt tggcctgttg ctgatccagg gtcttgcgca
    27421 gcatcgcgat ggagactggg tcggccccat ttgcagcgga cgtggagggg gtgggtgcga
    27481 tgttcatgat gtcagtcgcg gaagttaccg tattgcaaag ggtagtccgt gatgtccttg
    27541 cgcaccaggg caatacattc ttgcaggacg tcccgcttgg cgccgctcac gcgtacggca
    27601 tcgccctgga tgctggcctg caccttgagc ttgctgtcct tgaggagctt gacgatcttc
    27661 ttggactgct cctgctcgat accgacgcgg accttcagct cctgcttgac cttgttgccg
    27721 cccaccttct gcacgtcacc gtggtcgagg cagcgcacat cgatcttctt ggcggtcatc
    27781 tcggggagca ggatggccag catctgttcg agctgaaaat cgctgtcgcc gtgcagggtg
    27841 agcagctttt cggtctgctc gacccgcgca tcgctgccct tgaagtcgta acggcccgtg
    27901 atcttgcggt tggcgccgtc cacggcgttg cgcagggcga ccatatcgac ttcggaggtg
    27961 atgtcgaaag acggcatggt cggctcccgc tcagccgaaa tggcagacgt agtggtaggg
    28021 ctcgccgctg acttcgatgt cgaaggcgga gttggcgggt acattgaagg attcgcccgc
    28081 gccataggtc ttccactcgg tttcaccctt caggcgcacg cggcaggcgc cagcgacgcc
    28141 ttccatgatc tcgggcgcac cggtgttgaa ggtgagcgtg gcgggcagga tcacgccgac
    28201 ggacttcttg gtgccatcgg cgagcacgat ggagtggctg acgcacttgc cgtcgaaata
    28261 gacattggcc ttcttgacga cggagacggt atcgaactgg acgttctggg tcatggtgag
    28321 cgttcctgaa aatttattcg atgcccagga gccgctggat cagccccttg gcgatgaaac
    28381 cgatgaatcc gacgcccaga ccgacaaaca tccagatcgc gccgtacttg ccggctttcg
    28441 attcccaggc aagctggccg atgatgaaga ccatgtaggc gatcagcccg ccgacgcaga
    28501 gcttgaggga gatgtcctcg aactcggcaa cggtgaggcc gaagaacaat ggggcgctcg
    28561 gatccatcat gagcgtgctc agccccgctt ggcccgcgcg ttggcggcga tgcgcatgcg
    28621 cagggcgttc aggcggatga agccgtgggc atccttctgg ttgtaggcgc cctggtcgtc
    28681 ctcgaaggtg gcgatggtcg gatcgaacag ggaatcggtc ttggagtccc gtgcgacaac
    28741 gatcacgttg cccttgtaga gcttgaggcg gacccagcca ttcaccgtct gctgggtctg
    28801 gtcgatcagg gcctgcaggg ccagccgctc gggcgaccac cagtagccgg tatagatcat
    28861 gctcgcgtaa cggggcatca ggtcgtcctt gaggtgggcg acttcccggt caagggtgat
    28921 ggactcgatg gcgcggtggg cgcgcagcag gatggtgccg cccggggttt cgtaacagcc
    28981 acgggacttc atgcccacgt agcggttctc caccagatcg aggcggccga tgccatgctt
    29041 gccgcccagc tcgttgagcc tggcgagcag ttcatggggc ttcatgcggg tgccgttgag
    29101 ggcgaccaga tcgcccttct cgaattccag atccaggtat tcggccgcat ccggcgccgc
    29161 ttcaggcgac acggtccagc gccacatgga ttcctcggcc tcggcggcgg ggtcttccag
    29221 gtgacggcct tcgaaggaga tgtgcagcag gttggcgtcc atggaatagg gggagccgcc
    29281 ctgcttgtgc ttcatctcga tgggaatgcc attcttctcc gcataggcca gaagcttttc
    29341 gcgggacagc agatcccact cgcgccacgg cgcaatgacc ttcacgttgg gcatcagcgc
    29401 gtagtagccc agctcaaaac gcacctggtc gttgcccttg ccggtggcgc cgtgggacac
    29461 cgcatcggcg ccagtggcgc gcgcgatctc gatctgacgc ttggcgatca gcggacgggc
    29521 gatggacgta cccagcaggt attcgccctc atagatcgtg ttgcagcgga acatggggaa
    29581 cacgaagtcc cgcacgaact cttcccgcag gtcgtcaatg aagatgttct ccggcttgat
    29641 gccgaactgc agggccttgg cacgggctgg ttcgagttcc tcgccctgtc ccaggtcggc
    29701 ggtgaaggtc accacctcgc attgatacgt gtcctgcagc catttgagga tgaccgaggt
    29761 atccagcccg ccggaatagg cgagcaccac cttctttacg tcgctcatgt cgtggttccg
    29821 ctcttgttac acatcacaat tcgtttgaca cccgggcagt ccggccgcca ctcagctttc
    29881 gaccttcccg atcagcagat attccatcag ggccttctgc acgtggagcc ggttctcggc
    29941 ttcatcccag accaccgact gcgggccgtc tatcacctcg gcggtgactt cctcgccccg
    30001 gtgagccggc aggcaatgca tgaacactgc acccggattg gcgacccgca tcatgtcttc
    30061 atctacgcgc cactcggcaa agtcgcgcat gcgctcttcg ttctctgcct cgaagcccat
    30121 gctggtccac acgtcggtgg tgaccagatc ggccccgcgg caggcttcca tcggatcgtc
    30181 aaagtgggca aagtggcccg tgccgatcag cccggcccgc tccggctcca cctcgtaacc
    30241 cggaggcgtg gagacgtgca catggaaatc cagcacctcg gccgcctgca gccacgtgtt
    30301 gcagacattg ttggaatctc ccacccaggc caccgtcttg ccctggatcg gaccgcagtg
    30361 ctcgatgaag gtgaagatgt cggccaggat ctggcaggga tggtattcgt tggtcagccc
    30421 gttgatcacg gggacgcggg aatactcggc aaagcgctcg atgatcgact gctcgaaggt
    30481 gcggatcatc accacatcgc tcatgcgtga gatgacctgg gccgcatcct cgacgggctc
    30541 gccacggccc agctgggagt cgcgggtatt caggtagatg gccgagccgc ccagctgctg
    30601 catgcccgcc tcgaaggaca gacgcgtgcg cgtactggcc ttttcgaaga tcattaccag
    30661 tgtccggtcg aacagcgggt gatagggttc gtaacgcttg aacttgtcct tgatccagcg
    30721 cgtgcgcgcg aacaggtaat cgtattcggc ccgggagaaa tcccggaact ggagataatg
    30781 tctcggtgct ttcatgggca ctcgttttct aggttattga ccagcaagaa attcctttac
    30841 cagcggcgcc agcccctcca ccagcgccgc ggcctcctcg gcgctgaaat tcagcggcgg
    30901 gagcagacgg atgaccttgt cagccgtcac gttgatcaga agaccggcct gcagggcgcg
    30961 ccccaccagt tcggcgcagg gacgatcgag ctcgatgccg atcataagac ccgcgccacg
    31021 aatatcgatc acgccgggca gcccggccag cgccacggca aacccttccc gcagggcctg
    31081 gcccatgcgt cctgcgttgt ccagcaggcg ctcgtcgtcc agcacggcca gggtttccag
    31141 tgccgcggca cacgccagcg gattgccgcc aaaggtggag ccgtggttgc ccggtttgaa
    31201 gacgcccgcc gcccgtcccg aagccaggca ggcgccgatc ggcacgcccg agcccagtcc
    31261 cttggcgagc gacatcacgt ccggcttgac gccggcgttc tgatggccaa accacttgcc
    31321 ggtccggccg acgccgcatt gcacctcgtc gaacatcaac agccatccgt tcgcgtcgca
    31381 catgccacgc agggcttcca tatatgccga gtgagccacg tgaatgccgc cctcgccctg
    31441 caccggctcc aacaggatcg ccacgatgtc cgaacgaccg gcagtcgcct gctccagcgc
    31501 tccgagatcg tcgaaaggaa cccttacgaa tccaccgacc agcggctcga agcccttctg
    31561 gaccttggcg ttgccggtag ccgacagggt tgccagcgtg cgtccgtgaa aggaatgctc
    31621 catcaccacg atggccggct cggcaatgcc tttgccgtgc ccgtacaatc gggccagctt
    31681 gatggccgct tcattggcct cgcagccgga attgcagaag aacacttcat ccatgcccgc
    31741 cagcgccgcg agccggtcgg aaagctgttc ctggcgcgga atggcgtaca gattggaggt
    31801 gtggatcacc tgtgcagcct gttcggcaat ggcgcgcacc aggcggggat gattgtgccc
    31861 gagtgtggag acagcaatgc ccgagagggc atcaaggtaa cgcttgccct cggtatcaaa
    31921 cagccaggat ccctgtccat ggctgaaagc caccggcagg cggccgtagg tattcatcag
    31981 gtgcggcatg gaaacccttg tttcataatc tgcccccccg cacccgagct gtgaagcggg
    32041 gagccggaaa aggcgaaacg gcgactcgcg ccgccgttta aatgcaatat cgcatcctaa
    32101 ttcaaaacct tagccttgta tagcgggcgc gtgtgtcggc gccccggcgc gacgcggcct
    32161 tccgtaacgc gaaatggcgt ctttcctgat agaatccagc ttccgcacgg atgcagtcca
    32221 gtcgccgcag agcctcggat tgcgagccgc ctttccgaca ccctccgcct gcacccatga
    32281 ttcaaaaact tgaacatttc atgattgtcg gccgcacctc cgacggcaag actttccgtc
    32341 cgagcgactg ggcggaccgg ttgtgcggaa tcatgtcggc cttcggcgcc gacaaccgca
    32401 tgacctactc gccctatgtc cgtccggcgt gcaatctccg gggcgacaag acggtattgg
    32461 tggacgcgcg cattcacgac atcgagccgc ttgcctacaa cttcctgaag aatttcgcca
    32521 aagacaacga ccttcaggtc gaagtgctgc cggacgatca cttcgactga gacgcgtggg
    32581 cgcgacggtg cctgctcccc gcacatcgcg acagcgcaaa aaaagccggc tcgaaagccg
    32641 gctttttcat ttagcgaacg cagcagtcag tgcttgacga cgccgccgtt gtcggccggt
    32701 tgatccgcag ccgcagcgac cgggacttcc ggcgccggcg tgtcgggcaa cggttcgggc
    32761 cgacgttcca gcgccagttc gagcacctcg tcaatccact tgacggggat gatctccagc
    32821 gcattcttga cgttgtccgg aatctcggtg agatccttga cgttctcgga cggaatcaaa
    32881 gcccgcgtga tgccaccgcg cacggccgcc agcagctttt ccttcaggcc gccgatgggc
    32941 agcacctcac cccgcagggt gatctcgccc gtcatcgcca cgtcacaccg caccgggatg
    33001 cccgtcagca ctgaaaccag gccggtgcag atggcgatgc ccgccgacgg accgtccttg
    33061 ggaatcgcgc cctccggcaa gtgaatgtgg atgtcggagt tctggtagaa atccgccgca
    33121 atgcccaggg acttggagcg acgccgcacc accgacaggg ccgcctggat ggactcctgc
    33181 atgacctcgc cgagcttgcc ggtggtcatg atcttgccct tgcccggcag cgccacggcc
    33241 tcgacggtga gcaattcacc acccacctcg gtccatgcga gcccggtgac ctgtccgatc
    33301 tggttctgct tttccgcaac accgaaggtg tacttgcgca cgcccagata cttgtcgatg
    33361 ttcttggcgt tgaccaccac cttggccttg cgcttgccga gcacgatcgc cttgacgacc
    33421 ttgcggcaga ccttggagat ctcacgctcc agcccgcgca caccagcctc gcgggtgtaa
    33481 aagcggacga tctcgcggat cgcgtcttcc gtcaccgaga gctcttcgcg cttgatgccg
    33541 ttgttcttca tctgcttcgg cacgagatag cgctgggcga tgttgacctt ttcgtcctcg
    33601 gtgtagccgg acagacggat cacctccatc cggtcgagca gcggcgccgg gatattgagg
    33661 gtgttggccg tcgccacgaa catcacgtcg gagaggtcgt aatccacctc gatgtagtgg
    33721 tctacaaagg tcgcgttctg ttccgggtcg agcacttcga gcagcgccga ggacggatca
    33781 ccgcggaagt cctgccccat cttgtccact tcgtcgagca ggaacagggg gttgcgcacg
    33841 cccactttgg tcatgttctg caggatcttg cccggcatgg agccgatgta ggtccggcgg
    33901 tgaccgcgga tctcggcttc gtcccgcacg ccgcccaggc tcatgcgcac gaacttgcga
    33961 ttcgtcgcgc gggccacgga ctggccgagg gaggtcttgc ctacacccgg agggccgacc
    34021 aggcacagga tcggcgcctt gagcttgtcg acgcgttgct gcaccgcaag gtattcgagg
    34081 atgcgttcct tgaccttctc gaggccgtaa tgatcctgat cgagcacctt ctcggcctcg
    34141 gccagatcct tgctgatacg cgacttcttc ttccacggca gacccaccag agtctcgatg
    34201 tagttgcgca ccacggtggc ttcggcggac atgggcgaca tcaggcgcag cttcttcagc
    34261 tcaccctggg ccttggcagc ggcttccttg gacatgccgg acgccttgat cttcttgtcc
    34321 atctcttcca gatcggcgcc gtcctcccct tcgccgagtt ctttctggat ggccttgacc
    34381 tgttcgttca ggtagtactc gcgctggctc ttctccatct ggcgcttgac gcggccgcgg
    34441 atgcgctttt ccacctgaag gatgtcgagt tcggtctcga gctggctgag cagacgctcc
    34501 aggcgttcgc ccggattgaa catctcgagg atttcctgct tctgctccag cttgaggggc
    34561 aggtgcgcgg caatggtgtc ggccagccga cccggctctt cgatgcccga cagggagctg
    34621 aggatctccg gcgggatctt cttgttgagc ttcacgtact ggtcgaactg gctgatgatc
    34681 gcccggcgca tggcctcgac ctcgttgttc gg
//
LOCUS       DAOQPA010000025        34181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_536_length_34181_cov_19.484674, whole genome shotgun sequence.
ACCESSION   DAOQPA010000025 DAOQPA010000000
VERSION     DAOQPA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 34181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34181
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_34181_cov_19.484674"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2592
                     /locus_tag="PKX01_05500"
     CDS             325..2592
                     /locus_tag="PKX01_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMZ75612.1"
                     /translation="MDERIRLAALEYHRLPKPGKISIAPTKALTNQQDLSLAYSPGVA
                     AACDAIVEDPAEAASLTARSNLIGVVTNGTAVLGLGNIGPLAAKPVMEGKGVLFKKFA
                     GIDVFDLEIDEPDADKLIDMIAALEPTFGGINLEDIKAPECFYIEQKLRERMKIPVFH
                     DDQHGTAIVVGAAFLNGLHLLGKDLKQVKLVTSGAGAAALACLDLLVMLGVPVENIWV
                     TDIEGVVYEGRTVLMDPIKARYAKTTSARTLGEVIEGADVFLGLSAGGVLKPAMVAKM
                     ASRPLIMALANPTPEILPEEVRKVRDDALMATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTITDEMKLAAVKAIAELARAEASDIVAAAYGQEVSGFGPEYIIPKPFDPRLIVKIA
                     PEVARAAMASGVATRPIADFDAYCQQLNNFVWQSGLVMKPVFQAAKRSPKRIIFAEGE
                     SERVLRAVQTVVDEGLCRPVLIGRPDVTLENIDRYGLRLRPELDFELVNPETDPRYDQ
                     LWTHYHALMERKGVSVDYAKREVRRRPTLVGALLLKFGYADGLICGTYGMHGLHLRFI
                     ETVLGRQTGVRNFYTMNLLSLPGRTVFLCDTYVNYDPTPEQVVEMTRLAADEVRRFGI
                     QPRIALLSHSSFGTDNSPTAEKMRKALVMLHAEHADLEVEGEMHGDAALDASIRQQIF
                     PNARMREDANLLIFPTLDAANIAFNLLKTAAGEGMTVGPILLGSAKPVHILTPTATVR
                     RIVNMTALTVVEAGR"
     gene            2747..3904
                     /locus_tag="PKX01_05505"
     CDS             2747..3904
                     /locus_tag="PKX01_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ75613.1"
                     /translation="MTDGRTALVLTGGGARAAYQVGVLVAIRDVWGKRSGNPFPILCG
                     TSAGAVNAVALAAYSADFDAAVRKLAFIWRNFHVDQVYRADTAAVVRSVTRWGSAIFL
                     GWLFHQVPRSLLDNEPLRALLTAMLDFGAIDRAIADGHLRAVSVTASGYTSGESLSFF
                     QGVPALEPWRRAQRVGLRASLAVDHLLASSAIPFVFPAVRLHREYFGDGSMRQQAPVS
                     PAIHLGARRILVIGAGRRGEEARQRTYDYPSPAQVAGHAMASIFLDGLTTDLERLERI
                     NATLAALPPGAREAAGIPLRPIETLVIAPSQRLEYIAARYRNALPPALRLVLRGVGAT
                     RRNGSMLLSYLLFERSYTRALVELGYRDAMARRGEIARFLGLDSVQGRSSD"
     gene            4122..5147
                     /gene="pbpG"
                     /locus_tag="PKX01_05510"
     CDS             4122..5147
                     /gene="pbpG"
                     /locus_tag="PKX01_05510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HMZ75614.1"
                     /translation="MNIRKSLLALAGAVALQATGPADALAATHRPAKKSFVAKAAPAP
                     AKNAKARNRAAARAAFASPQVQRTAALAVAPEMDHEGNPVLASAAFMVADQATGTVLL
                     EKNSDAVLPIASITKLMTAMVVLDARLNLGEVLTVANDDVDTLRGSSSHLPVGVDLTR
                     EDMLRLALMSSENRAASALARHYPGGLPAFVAAMNRKAVSLGLKDTRFSDSTGLNSAN
                     VSSARDLVKMVSAASRYPLIREFSTTGNYTVYLNGRPRQFGNTNALVKSPDWQIGVSK
                     TGFINESGKCLVMQAWLLNKPMIIVLLDSFGRFTRVADAQRVKRWLESAAAQQRLAEV
                     VRGGPTT"
     gene            complement(5211..5942)
                     /locus_tag="PKX01_05515"
     CDS             complement(5211..5942)
                     /locus_tag="PKX01_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HMZ75615.1"
                     /translation="MMKLLDVLAEYSDPVPLKQLALETGLHPSTAHRILGAMSGSGFV
                     ERSEAGAYRLGIRLLELGSLVKSRISLRETAMPAMLRLHAETGESVNLGIRAGDEIVY
                     VERTSSGRSSVRVVHIVGARAPLHTTATGKLFLVEDGAHQVKEYARRTGLPGTTPTSI
                     TTLAALEKELDKVRRHGVAYDLDEVENGVRCIAAGIRDDSGELVAGLSLSTPAERFNP
                     DWAPLIKATADEISRALGYVPPAGR"
     gene            complement(6143..7084)
                     /locus_tag="PKX01_05520"
     CDS             complement(6143..7084)
                     /locus_tag="PKX01_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HMZ75616.1"
                     /translation="MDLLIDVILKAGRSAVELSFFVLLPVMVVMLSLMRLLEARGVLD
                     WLVEKLAPALRPLGLTGLGVFAALQINFVSFAAPVATLAMMDQRGASSRHLAATLAMV
                     MAMAQANVSMPMAAMGLHFGIVLGWSLVGGLVAAAATYYGFGRHLSAVEHTLDETLNH
                     PVAESAKGVLDVINRAGAEAFKIAVGAIPMLVLSLTVVLALRRLGALDVLTGLLAPIL
                     LAVGADPALILPTLTKYLAGGTAMMGVMDEMLRAGTATAATLNGASAGLLIHPLDIPG
                     VAVLISAGRRVADVWKPAALGAGVGILARVAGHLVVG"
     gene            complement(7088..7822)
                     /locus_tag="PKX01_05525"
     CDS             complement(7088..7822)
                     /locus_tag="PKX01_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HMZ75617.1"
                     /translation="MSSGMTEIWVYLSAQPLLWLSLTLAAYLVALTLHRKAGAHPAVN
                     PVLLSVLILVGLLVATGTPYQHYFDGAQFVHFLLGPTTVALAVPLHAQLKRLVAMGGP
                     LLIALAVGSLTAGLSAYAIGALLGASTPTVISLAPKSVTTPIAMGIAERLGGLASLTA
                     VFVILTGIFGAIFATGLLHRLGVKDEASTGFALGLASHGIGTARAFQLSEQTGAFAAL
                     AMGLNGLFTALTLPWLLPLLAPLLSR"
     gene            complement(7819..8175)
                     /locus_tag="PKX01_05530"
     CDS             complement(7819..8175)
                     /locus_tag="PKX01_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HMZ75618.1"
                     /translation="MIAAFALLLGFQLAGETLRVALHLPVPGPVMGMALLLVYLIFRG
                     GPDDGLRTTSGGLLQHLSLLFVPAGTGVMLHAGRLADEAGPILVALVASTVLGLAATG
                     LTLHALTRRSRQEDAQ"
     gene            complement(8249..9352)
                     /locus_tag="PKX01_05535"
     CDS             complement(8249..9352)
                     /locus_tag="PKX01_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HMZ75619.1"
                     /translation="MERRSFLKKASAGVAAGAVAAPALAAELPTIKWRLASSFPKSLD
                     TIFGGADVVAKRVAAATGGKFQIQVFAAGEIVPGPGVMDAVKDGTVECGHTCSYYFVG
                     KDPTFALDTAIPFGLNSRQQTAWMMDGGGLELFREFFKDYNIYNIPCGNTGAQMGGWF
                     RKEIKSVEDLKGLKFRCGGFAGQVLARLGVVPQQIPGGDIYPALEKGTIDAAEWIGPY
                     DDQKLGFNKVAKYYYYPGFWEGGPQISLYVNMKQWAALPKEYQTILEDACLYAHAEMQ
                     ARYDAKNPGALKQLVGSGTQLRPFPNDVLAAAYKAANDLYDETAASNPKFKKIYEAWK
                     KFRDDQLQWFAVAENRFDNFMQAARNASAKAKK"
     gene            complement(9450..10556)
                     /locus_tag="PKX01_05540"
     CDS             complement(9450..10556)
                     /locus_tag="PKX01_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HMZ75620.1"
                     /translation="MERRRFLTQAGAGMAAGALAAPAAAAMADLPILKWRLASSFPKS
                     LDSIHSSTESLARQVALLTGGRFQIQVFAAGELVPSAAVLDAVKDGTVEIGHSASYYY
                     VGKDPTFALDTGIPFGLNSRQQMAWMHDGGGLELLRDFFREYAIHNIPCGATGTQMGG
                     WFRKEIRSVEDLKGLKFRVGGFAGQVLARLGVVPQQIPAGDIYAALEKGAIDAAEWVG
                     PYDDLKLGFQRVAKYYYYPGFWEGGPQLTVYVNLKHWNALPPEYQAALENACALAHVE
                     MQARYDARSPAALRQLIASGTQLRPFSHEIMSAAYQAAHALYDETAAKNPRFRKIYEP
                     WKKFRDDQLQWFAVAESHFDAFMQATRAAARSRK"
     gene            complement(10594..11934)
                     /gene="pmbA"
                     /locus_tag="PKX01_05545"
     CDS             complement(10594..11934)
                     /gene="pmbA"
                     /locus_tag="PKX01_05545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HMZ75621.1"
                     /translation="MAEKRFSFTQDELRTLADDILKYARKGGASACEVDVSEGFGQSV
                     TVRRDEVDTIEYNRDKGIGVTVYDGQRRGYASTSDFSAASLKATVAAALSIARFTAPD
                     PAAGLPDAVLLARDIPDFDLYHPWALSSEDAIELARRCERAAFAVSPEIRNSDGASVS
                     MQESHFISANSLGFMGGYSSSRHFLSCSVIAGEGDDMQRDDWYSSKRDPAELADAEEV
                     GTLAARRALARLGARQIPTCEVPVLFEAPLAAGLIGAFVHAVSGGALYRKSSFLLDSL
                     GKPVFPGHIQISERPHLPKAFASSAFDDDGVATRDREVIVDGMLQGYFLSTYSARKLG
                     LETTGNAGGSHNLLVKPGAHDLDGLVRQMDRGLLVTELLGHGVNYVTGDYSRGAAGFW
                     VENGQIQFPVHEITIAGNLKAMLAGIREIGSDVLVRGSKHCGSILVDRMTVAGA"
     gene            12004..12552
                     /gene="yjgA"
                     /locus_tag="PKX01_05550"
     CDS             12004..12552
                     /gene="yjgA"
                     /locus_tag="PKX01_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HMZ75622.1"
                     /translation="MAHPHSHHDDDEDTGPSKSQLKRDSQALQELGQELAALGKERLA
                     KVPLDEDLRDAVRDYQRFPSHEAKRRQLQYIGKLMRHVDPAPIRAALDAFKGVSAEEN
                     ARMHRLEALRTRLLDDEKTLHDIATAHPGADLQHLRVLRRNALKERELGKPPRAFREL
                     FRVLRELEGAPDNDPPSGDDEA"
     gene            12549..13136
                     /gene="mog"
                     /locus_tag="PKX01_05555"
     CDS             12549..13136
                     /gene="mog"
                     /locus_tag="PKX01_05555"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HMZ75623.1"
                     /translation="MSETAIIGLVSISDRASEGVYQDKGIPALQDWLGSALASPWRAE
                     TRLIPDERAQIEATLIELCDGVGCDLVLTTGGTGPALRDVTPEATLAVGHKEMPGFGE
                     QMRQVSLAFVPTAILSRQVAVIRGKTLIINLPGQPKAIAETLEGLKDGEGRPTVHGIF
                     AAVPYCIDLIGGAYIETHDAVIKAFRPKSAIRPKA"
     gene            13197..13649
                     /locus_tag="PKX01_05560"
     CDS             13197..13649
                     /locus_tag="PKX01_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HMZ75624.1"
                     /translation="MSDLSFQHSLFARLRALEESAFPKTCANCGRKYRTHEEFLTATH
                     PVRRDHTGLKQSYDDDGAVIVDVFRNCVCGSTLLESFHDRRDTSPAGQKRRARFAEML
                     SSLVAAGLSESVVRAELLLLMRGKPNRLVELIRSVKDGAGGGDGLPLP"
     gene            complement(13666..14268)
                     /locus_tag="PKX01_05565"
     CDS             complement(13666..14268)
                     /locus_tag="PKX01_05565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ75625.1"
                     /translation="MSLDHFAHKADSFDANPVRVDNVDAIARVLRDTVALDPAMRLLD
                     FGSGTGLLLERLAPHVAHITAVDVSPAMNRQLDAKRAHLPCTVEILELDLVAHDLAQT
                     FDGIVSSMTLHHVADVAALLRKFHGLLPDGGFIALADLDTEDGSFHSEDTGVFHAGFE
                     RAYIAGLAAAAGFREVQVHSASVVRKPQGDYPVFLLTAVR"
     gene            complement(14318..14986)
                     /locus_tag="PKX01_05570"
     CDS             complement(14318..14986)
                     /locus_tag="PKX01_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HMZ75626.1"
                     /translation="MNPDTRQLALLKTALFALCLVPAVLLGRAFSLDGLGANPIEALT
                     RATGEWTLRLLLITLCVTPLRKLTGWHWLVRLRRMLGLFTFAYACTHLLTYLWLDQFF
                     DWHAIAKDILKRPFITVGMAAFVLMAPLAATSSNAAIRRLGGRRWQALHRSIYPIAIL
                     ACVHFWVLVKKDITEPLLYALITAVLLGLRAWWREQERRRQLAGAYAPPPQARGKVIP
                     IVAK"
     gene            complement(14993..15919)
                     /gene="msrP"
                     /locus_tag="PKX01_05575"
     CDS             complement(14993..15919)
                     /gene="msrP"
                     /locus_tag="PKX01_05575"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HMZ75627.1"
                     /translation="MLIRRPADLPPSEITDPAVYGARRRFMGQAGALALGSLLPAAGQ
                     AALGPLSPLSHSALSTTETPTSLRAVTGYNNFYELGTSKTDPAENTFRLKPAPWTLRV
                     EGLVNKPRTFALEDLFRLAPLEERIYRMRCVEGWSMVIPWVGLPLASVLKLADPQGSA
                     KYVEFTSHYDRATMTRGVLDWPYSEGLRLDEAMHPLTILAVGLYGEVLPVQNGAPVRL
                     VVPWKYGFKGAKSLVSLRLVDKPPLTAWMKAGPSEYGFYANVNPDVDHPRWSQATERR
                     IGEFLKRKTLMFNGYADQVASLYTGMDLRRYF"
     gene            complement(15991..16770)
                     /locus_tag="PKX01_05580"
     CDS             complement(15991..16770)
                     /locus_tag="PKX01_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HMZ75628.1"
                     /translation="MNTPPDRDRLHAFADDRLPADERVRVAGWLADHPDSAAAVHDWR
                     RQNAALHAAYDGVLEQSVPPHLLAPPTPTPRPLLRVAAVLGWLALGIVIGRVLPPDGP
                     AATVDDPLAALPHRAAVAYAVYAPEVRHPVEVGADQESHLVAWLSKRLGTPLRAPHLV
                     AEGFRLVGGRLIPSEQGPGALFMYEDGTGGRLTLYVVTVEHDGGETAFRFAHAGALST
                     FYWVDRHTAYALSGELPRASLLPVASAVYRQLGEDRPPAQK"
     gene            complement(16767..17267)
                     /locus_tag="PKX01_05585"
     CDS             complement(16767..17267)
                     /locus_tag="PKX01_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ75629.1"
                     /translation="MSFRQAIVAEIPRLRRYARALTGDASRADDLVQDTLERALGKWR
                     LWRPGNLRAWLFTLMHNIFVNQLQESVLVISLDGDTHLDVPVRPRQQDNLELRDLDRA
                     LARLSPEQREVLLLVGLEELSYEDTARVIGVPVGTVMSRLARARDKLRLLLAGDERAL
                     HLKVVK"
     gene            complement(17288..17680)
                     /locus_tag="PKX01_05590"
     CDS             complement(17288..17680)
                     /locus_tag="PKX01_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75630.1"
                     /translation="MMHRSVTHALIGLGALLAAACTTLPGLATAPAQSRDGLLADAKG
                     MTLYVFDKDAAGSGKSACNGPCANNWPPLVAAEGDKASGDWSVIVRDDGRRQWAFKGK
                     PVYTWVKDQKPGDTTGDGFNSVWHVARP"
     gene            17950..18693
                     /gene="surE"
                     /locus_tag="PKX01_05595"
     CDS             17950..18693
                     /gene="surE"
                     /locus_tag="PKX01_05595"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ75631.1"
                     /translation="MRILLSNDDGYFAPGLQALASALGDVGEISVVAPERDRSGASNS
                     LTLDRPLSLRRAANGFHFVNGTPTDCVHLAVTGMLEQLPDIVVSGINHGANMGDDTIY
                     SGTVAAATEGYLLGVPAIAMSLVSKEASDFSAAARIARELVARFIRQPVRQPTLLNVN
                     VPDLPYDQIKGIRVTRLGKRHKAEPTIKSVTPRGETVYWVGAAGAAQDAGEGTDFHAV
                     AHGYVSVTPLQIDLTHAGQIAPVQDWLAS"
     gene            18690..19349
                     /locus_tag="PKX01_05600"
     CDS             18690..19349
                     /locus_tag="PKX01_05600"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMZ75632.1"
                     /translation="MSEVARVGVHGAQRARARMVERLREQGVRDERVLAALLHVPRHL
                     FVEEALASRAYEDTALPLGLQQTISQPFVVARMLELLLAGRESLGKTLEVGTGCGYQA
                     AVMASLASDVYSVERLRLLLDRARANLRHLRLPNVRLKHADGSVGLKDAAPFDTIIVA
                     AAAPKVPPALVEQLAEGGRLILPVGMAEQVLVLIERTVRGPKETRLSPVRFVPLLPGT
                     E"
     gene            19367..20350
                     /locus_tag="PKX01_05605"
     CDS             19367..20350
                     /locus_tag="PKX01_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ75633.1"
                     /translation="MLSRLSLSLFLSALALAGCATKSPAPVADRAKAGATPPVRAETP
                     AAPAPVVARPGYYIVKPGDTLIRIALDHGQNYRDIAAWSGLENPNLIEVGQELRVKPP
                     ESGAVVKPVAPAGADVRPVAPSADGLRREPKGGKVPYSEQAWAQAQKPEAPVVPVKAD
                     AKAEPRTDARPDAKPDVKPETKPEAKPETADDKGKDAAKPAAAGDDRIEWSWPAGGKV
                     MAGFNDTSSKGIDLSGKPGDPVYAAAAGKVVYAGTGLRGYGKLLIVKHDNAYLSAYAH
                     NQQLLVKEGQSVSKGQKIAELGDTDSDRPKLHFEIRKQGKPVDPVKYLPSR"
     gene            20495..21430
                     /gene="rpoS"
                     /locus_tag="PKX01_05610"
     CDS             20495..21430
                     /gene="rpoS"
                     /locus_tag="PKX01_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HMZ75634.1"
                     /translation="MYEPGVPEEELETPEPDLPPEVEVFLETPAPVDTEFLGDVTQLY
                     LNEIGANPLLTAEEELSLSRRVREGDFPARQTMIERNLRLVVNIAKHYLNRGIPLLDL
                     VEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELNQ
                     VLRTQRQLEASGHGDTTVEEVARQLSRPVEDVRAILALNEHTASLDAPLDIDPSLSIG
                     ESLADEGAETPDTQIHGLEVESLVREWIGQLNEKQRMVIRHRYGIDECEVQTLEELAD
                     SLDLTRERVRQIQLEALGQLRRIIKRRGVSKDVLF"
     gene            21445..21795
                     /gene="arsC"
                     /locus_tag="PKX01_05615"
     CDS             21445..21795
                     /gene="arsC"
                     /locus_tag="PKX01_05615"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765798.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HMZ75635.1"
                     /translation="MTDTIRLYHNARCSKSRSACALLADKGVPTTIIEYLKTPPTREE
                     LAAVLAALGMRAEDLVRKGEDVFKATFKGRTLSEDEWLDALVAHPILIERPIAVRGAR
                     AVVGRPPEKVLELL"
     gene            complement(21853..22866)
                     /gene="mltB"
                     /locus_tag="PKX01_05620"
     CDS             complement(21853..22866)
                     /gene="mltB"
                     /locus_tag="PKX01_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HMZ75636.1"
                     /translation="MILRPFRLRPALLLASALAAGPLAAAERYADRPDVQQFIAEMKE
                     RHGFDPDALTFIFRRATFQPAVIKAISPPKDPGVRSWQRYRGRFIEPVRIKAGVRFWD
                     AYAETIKAASDKYGVPEEIIVGIIGVETIYGRNTGSFQAVSALSTLAFDYPRRAELFR
                     GELESLLLMAREQGRDPLDYQGSYAGALGLPQFLPSSVRNYAVDFDGDGHIDLLASPK
                     DAIGSVARYLQMHGWERGGPVAIRAVTNADATLETLIANDITPAFDAEALAAHGVHAA
                     SGTPAVGPAAFVQLVTPGADSEYWLGYQNFYVITRYNRSSFYAMSVFQLAEAVKEARQ
                     ARR"
     gene            complement(22863..24803)
                     /locus_tag="PKX01_05625"
     CDS             complement(22863..24803)
                     /locus_tag="PKX01_05625"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and DUF4129 domain-containing
                     transglutaminase family protein"
                     /protein_id="HMZ75637.1"
                     /translation="MAERGLRPDQTGWLVVAAGVSLLPHAAQLPHWLSAVCALFLSWR
                     GMLAAQRARLPHRALLGTLGLAVAGGVLVEYGHLFGKDPGIALLAAFLGLKLLETDDI
                     RGGRAVILLTFFLQMGQFLHQQGMATAALALVGTVVSLATLAALAGRARPLRPRLTEA
                     ARLTVLSLPFMAVLFILFPRVQGPLWGLPADAFSALTGLSDTMTPGALARLSESGAIA
                     FRATFDGPPPPRAQRYWRGPVLTRFDGHTWRAAHLPARDVPAYAVSGRAYTYTLTLEP
                     HDRPWLLALDFPDAQAGLRYGADFQLLAREPVRSRLRQTLASYPDTPVGLDEPPILRR
                     LALALPAGANPRTVAAGSQLRANAVRADDIPARGIALMRDARLTYTLEPPPLGEDDVD
                     AFLFDTRQGFCEHFAAAFVVLMRAAGVPARVVTGYQGGEINPVDGSLEVRQSDAHAWA
                     EVWLAGRGWLRVDPTAASAPARIERGLAAALPDTGHLPLLMRPELAWLQGLQHHLDAL
                     NHRWNQWVLGYNPARQRDLLAGLGLGEVDWPQLTGLMTAACAAVTAALAGVLLRNRRR
                     DPVLRAWEGVCSRLAAHGLPRADWEGPTAYAARVGTTRPDLADAIAGLATDFARLRYG
                     EASDTAARRDRFLHSARRFRPR"
     gene            complement(24796..25782)
                     /locus_tag="PKX01_05630"
     CDS             complement(24796..25782)
                     /locus_tag="PKX01_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ75638.1"
                     /translation="MPVHARVDALRARFQRWLFRVHGPEPAPIRLGQRRIFVMPTAFG
                     VALGVTLLVMLLASINYALSLGYALTFLLGGVGWVHIHLSFRNLVGLSIAPGRVEPVF
                     AGATASFGVLMTNPLPRSRLALRLWPARTGASPDEAFELPPDSQVRISVHVAAPQRGW
                     LPLPRLQLESRHPLGLIRAWSLFQPDLRALVFPTPEADPPPLPRGTGLRPGAHGRRQG
                     RDDFAGLRAHQPADPPRHVAWKALARSGEWLTKQFDGGGAEALWLDWNTLPPGLDTEQ
                     RLARLTRWLCDAEAAGLEYGLRMPQQTFPPASGPAHRLACLTALALFGGPDG"
     gene            complement(25782..26699)
                     /locus_tag="PKX01_05635"
     CDS             complement(25782..26699)
                     /locus_tag="PKX01_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ75639.1"
                     /translation="MPLYIANRLVDAAGRILLGKEQAIRLALACLLARGHLLIEDVPG
                     VGKTTLAHLLARLLGLQFQRIQFTSDLLPADILGVSVFDPRDGAFRFHPGPVFAQMIL
                     ADEINRATPKAQSALLEAMEEGQVSAEGRTLPLPAPFFVIATQNPADQIGTFPLPESQ
                     LDRFLMCIALGYPDPAAERALLAGEDRRALLARQETLLAPGELDTLFDAAARVHVAPP
                     LLDYVQALLAHSRQATGFSLGLSPRAGLGLVASARAWALLAGRDHVQPEDVQAVLPAV
                     AGHRLRSDGDRRTSPEERVRRLLEAVPLP"
     gene            26868..27794
                     /locus_tag="PKX01_05640"
     CDS             26868..27794
                     /locus_tag="PKX01_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HMZ75640.1"
                     /translation="MTSTAFITHRECWLHNMGAHHPESPERLAAINDRLIAAGLDMYL
                     SFYDAPVATAEQLSRAHPREHVDAILASAPEHGIRHLDPDTAVCPHSIKAALRSAGAG
                     VMATDLVMSGEVENAFCAVRPPGHHAEAAKAMGFCFFNNVAVAARHALDVHGLKRVAV
                     VDFDVHHGNGTEDIFKDDPRVLMVSIFQHPFYPYSGHDNPAPNMVNVPVKAGSRGDVF
                     REIVTNIWVPALRNHAPEAIFISAGFDAHYEDDMGSLGLVEKDYIFATEELKKVAAEF
                     SKRRIVSMLEGGYSLSSLARSVVAHVKSLADL"
     gene            27967..28845
                     /gene="dapA"
                     /locus_tag="PKX01_05645"
     CDS             27967..28845
                     /gene="dapA"
                     /locus_tag="PKX01_05645"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMZ75641.1"
                     /translation="MITGSIVAIVTPMNDDGSLDLPRLRTLIDFHVQEGTDGIVVVGT
                     TGESPTVDVDEHCELIRVCVEHAAGRIPVIAGTGANSTREAIELTEFSRKAGAVAGLS
                     VVPYYNRPTQEGLYRHFRAIAEASELPLILYNVPGRTVADLANDTALRLAEIPNIIGI
                     KDATGSLDRACDLIARAPRDFALYSGDDMTMMAFILLGGHGTISVTANVAPGLMHEVC
                     AAALQGDAVTARAANAKLVGLHRDLFCEANPIPVKWAVQQMGLIGPGIRLPLTRLSES
                     CHERVRKAMRQAGIEV"
     gene            28875..30041
                     /gene="bamC"
                     /locus_tag="PKX01_05650"
     CDS             28875..30041
                     /gene="bamC"
                     /locus_tag="PKX01_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HMZ75642.1"
                     /translation="MHSRTLASGSAVAMAIALAGCSGSLLESKKIDYKSASAAPVKSL
                     EIPPDLTAPTRDDRYTVPDVSPKGTATFSAYSAERTGQGAQVGATPGAAPTAASASGS
                     DKMHIERAGSQRWLVVQGSADKLWPQIRDFWQESGFLLNVDKPEIGVMETDWAENRAK
                     IEQGFIRNTLGKVLDGLYSTPERDKFRTRLEAGAEAGTVEIYISHRGMMEIYPTEGKD
                     RTIWQPRAADPELEAEFLQRLMVRLGADESRAKAQLATAPSSDKARLANAADGATLLT
                     VQDAFDRAWRRVGLALDRVGFTVEDRDRSQGLYYVRYVDPEVDVKKKGDEGLLSKLAF
                     WRSDKPALPTGSQYRIFVKNAGDESSVQVLTREGGVDKSDSARKILGLLYEQLK"
     gene            30207..30605
                     /locus_tag="PKX01_05655"
     CDS             30207..30605
                     /locus_tag="PKX01_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75643.1"
                     /translation="MCSINGYKKSQPAGWLFSLPEEGLLLVGSRSGGSSFSGLGVSSR
                     GSGSGRGSGFSRLGVSSRGSRGRSSSGSRSSGRSRRFNGLGFFLLAASGNGQGDESSN
                     EERLFHCWFPLSISEFNGLKIRSNQVGDPI"
     gene            complement(30700..31830)
                     /locus_tag="PKX01_05660"
     CDS             complement(30700..31830)
                     /locus_tag="PKX01_05660"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ75644.1"
                     /translation="MIDTLLGGLSVEQFLAEYWQKKPLLIRQAIPGFTGVVSRDDLFA
                     LARDPDVESRFIAHDGARWEVLHGPQKATDLRRKKQPWTVLVQGLNLFVPEGDALLHR
                     FDFIPQARLDDLMVSYAVDGGGVGPHFDNYDVFLLQGIGQRRWLISNQDDRSLIEDVP
                     LKILQDFRPVHDWVLESGDMLYLPPHWAHNGIALGECTTYSIGFRSPTYQELAQEFLV
                     YLQDAVQLEGLYADPDLKPHVHSAEIGDAMVDRVAEQLARITWTREDVRRFLGAYLTE
                     PKPHVFFDSPDEPLDHDSFLAQCNRAGYVLDARSLLLFSDGRFFLNGEAVSISHTDLP
                     VMIELADRRRISTVADLTQDGQALLYDWYCCGFAHPDSIESK"
     gene            31909..33222
                     /gene="rlmD"
                     /locus_tag="PKX01_05665"
     CDS             31909..33222
                     /gene="rlmD"
                     /locus_tag="PKX01_05665"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMZ75645.1"
                     /translation="MHVDAPREALIESLDHDGRGVARVDGKTVFIEGALPGERVEFVS
                     YRVKPKFELADVTRIVAPSAQRVAPPCVLFGECGGCSMQHLEPSAQLAAKQRVLENAL
                     WHLARVKADVLYPVISGAPWGYRFRARLSVRQVPSKGGVRIGFHQRRSSHIVDMRSCP
                     VLPTHVSVLLPALRDLVTALSVADRLPQIEVAVGEGLTVLVFRHLEPLTAADKDHLRA
                     FAESHGVQVWLQPFGPDSASRLHPVKAPGLAYTLPEFGVSMAFRPTDFTQVNMGINRL
                     LMRRAMLLLDPRPGERIADLFCGLGNFSLPIARLGATVVGVEGSDALVRRAAENAAAN
                     GLAERSEFHAANLFEATEDSLAALGRLDKLLIDPPREGAIAVVKALGADSPRRVVYVS
                     CNPATLARDAAVLVHEKGYRLKGAGIANMFPQTSHVESIALFERD"
     gene            complement(33284..33970)
                     /locus_tag="PKX01_05670"
     CDS             complement(33284..33970)
                     /locus_tag="PKX01_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HMZ75646.1"
                     /translation="MQIDTYGQQTQMLGASTNRVLRNTYLLLALSMVPTIAGAMLGVQ
                     MQFSFFAGSPLMGFLLFMGISFAFFWGIERNKNSGLGVALLLGFTFFMGLMLSSLLRV
                     ALGFSNGGTLIALAGGGTAAIFGVMATVATVSKRDFSGLGKFLFVGVIVLLLASVANI
                     FFQVPALYLTISAIAVALFSAYILYDINDIVRGGETNYVTATLRVYLDVYNVFVNLLN
                     LLMAFGGDRD"
BASE COUNT         5750 a        10837 c        12132 g         5462 t
ORIGIN      
        1 ggcgcggcgg caatggctgc ttcggcaatg ctttgagaca tcagacgatt ctccaggacg
       61 ttacagattc gtgagttgct ctgtccggcg gtgagagggc ggccgccggg cggcgatcgc
      121 gtctttgggc catcaaggct cggcgcgtat tgttggcgag tgtgcgggcg attccccaaa
      181 ggaggaagcc taatattatg ccacggttcg gtcgaggaca aaaatcacgc atttcgcatg
      241 aaatgctgtg gaagtcacgg atggccaaaa tgtccgttta gtcggatcat cgcagcacac
      301 ccacaccgaa gagggagaca ccccatggac gaacgcatcc gccttgccgc gctggaatac
      361 caccgccttc ccaagcccgg caagatctcc atcgcgccca ccaaggcgct caccaaccag
      421 caggacttgt ccctggccta ttcgcccggc gtggcggcgg cctgcgatgc catcgtcgaa
      481 gacccggccg aagcggccag cctgacggca cgcagtaacc tgatcggcgt ggtgaccaac
      541 ggcaccgcgg tgctgggcct gggcaacatc ggtccgctgg cggccaagcc ggtgatggaa
      601 ggcaagggcg tgctgttcaa gaagttcgcc ggcatcgacg tcttcgacct ggagatcgac
      661 gagcccgacg cggacaagct gatcgacatg atcgccgcac tggagcccac cttcggcggc
      721 atcaacctgg aggacatcaa ggcgcccgag tgcttctaca tcgagcagaa gctgcgcgag
      781 cggatgaaga tcccggtctt ccacgacgac cagcacggca cggccatcgt ggtgggcgcg
      841 gccttcctga acggtctgca tctgctgggc aaggatctga agcaggtcaa gctggtgacc
      901 tccggcgccg gtgccgcggc gctggcctgc ctggatctgc tggtgatgct gggcgtgccg
      961 gtggagaaca tctgggtgac ggacatcgag ggcgtggtct atgaaggccg taccgtgctg
     1021 atggacccca tcaaggcccg ctacgccaag acgacctcgg cgcgtaccct gggcgaggtc
     1081 atcgagggcg ccgacgtttt cctcggtctg tcggccggcg gggtgctcaa gcccgccatg
     1141 gtggcgaaga tggcctcgcg gccgctgatc atggccctgg ccaaccctac gcccgagatc
     1201 ctgcccgaag aggtgcgcaa ggtgcgtgac gacgcgctga tggccaccgg ccgttcggat
     1261 tacccgaacc aggtcaataa cgtcctgtgc ttccccttca tcttccgcgg ggcactggac
     1321 gtcggcgcga ccacgatcac ggatgaaatg aagctggccg cggtcaaggc catcgccgag
     1381 ttggcccgtg ccgaggcctc ggacatcgtc gccgctgcct acggccagga agtgtccggc
     1441 ttcgggcccg agtacatcat ccccaagccc ttcgatccgc gcctgatcgt caagatcgcg
     1501 ccggaggtgg cccgcgccgc gatggcgtct ggcgtggcga cgcggcccat cgccgacttc
     1561 gacgcctact gtcagcagct caacaatttc gtatggcaat ccggcctcgt catgaagccg
     1621 gtctttcagg ccgccaagcg ctcgcccaag cgcatcatct ttgccgaggg cgagtcggag
     1681 cgggtgctgc gcgcggtgca gacggtcgtg gacgagggcc tgtgccgtcc ggtcctgatc
     1741 ggtcggcccg acgtgacgct ggaaaacatc gatcgttacg gcctgcgcct gcgccccgaa
     1801 ctggatttcg aactggtcaa tcccgagacg gatccgcgct acgaccagct gtggacccac
     1861 taccatgccc tgatggagcg caagggtgtg tccgtggatt acgccaagcg tgaggtgcgt
     1921 cgccgaccca ccctggtggg cgcgctgctc ctcaagttcg gctacgcaga tggattgatc
     1981 tgcggcacct acggcatgca cggcctgcac ctgcgcttca tcgagacggt gctcggccgc
     2041 cagaccggcg tgcgtaactt ctacacgatg aatctgctgt ctttgccggg acgcacggtg
     2101 tttctgtgcg acacctacgt caattacgac ccgacaccgg aacaggtggt ggagatgacc
     2161 cgcctcgccg ccgacgaggt ccgtcgtttc ggcatccagc cccgcattgc gctgctgtcc
     2221 cattccagtt tcggtacgga caattcgccg acggccgaga agatgcgcaa ggccctggta
     2281 atgcttcacg cggaacacgc cgatctggag gtggaaggcg agatgcacgg cgacgccgcg
     2341 ctggatgcga gcatccgcca gcagatcttc cccaatgccc gcatgcggga ggacgccaac
     2401 ctgctgatct tccccacgct cgacgcggcc aacatcgcct tcaatctgct caagacggct
     2461 gccggcgagg gcatgacggt ggggccgatc ctgctgggtt cggccaagcc ggtgcacatc
     2521 ctcaccccca ccgccacggt gcggcgcatc gtcaacatga cggcattgac cgtcgtcgaa
     2581 gccggccgct gagtcgtcgg tacggccgcc ggggccgcgt gatggcgccc gcggccgggt
     2641 caaccctgcg ccgacgctcc aggcggccgt tatcactgct tgaaaggggc atgacgcccg
     2701 cggtcgcggg tgggcgtgac ggaatgtcgg cgcacggagg aggggcatga cggacgggcg
     2761 gacggcgctg gtactcacgg gcggtggcgc tcgggcggcc tatcaggtgg gcgtcctggt
     2821 ggccatccgc gatgtctggg gcaaacgctc cggcaatcca ttcccgatcc tgtgcggaac
     2881 gtcagcgggt gccgtcaatg cggtggccct cgcggcctat tcggccgact tcgatgcggc
     2941 tgtgcgcaag ctggcgttca tctggcgcaa ctttcacgtg gatcaggtgt atcgcgccga
     3001 cactgcggcg gtggtgcgct cggtcacgcg ctgggggtcg gcgatcttcc tgggctggtt
     3061 gttccatcag gtgccccgct ccctgctgga caacgagccg cttcgcgcgc tgctgacggc
     3121 gatgctggat ttcggcgcca tcgaccgcgc catcgcggac ggccatctgc gtgccgtgtc
     3181 cgtcacggcg tccggctata cctcgggcga gagcctgtcg ttctttcagg gcgtcccggc
     3241 gctggagccc tggcgacgcg cccagcgggt cgggctgcgc gccagtctgg cggtcgatca
     3301 tctgctggcc tccagcgcga ttccgttcgt gttcccggcg gtccggctcc accgggaata
     3361 cttcggtgat ggctccatgc gccagcaggc acccgtcagt ccggccatcc atctgggcgc
     3421 acggcgcatt ctggtgatcg gcgccgggcg gcgtggcgag gaggcgcggc agcggaccta
     3481 cgactacccg tcgccggccc aggtggcggg gcacgcgatg gcgagcatct tcctggatgg
     3541 gctgaccacc gatctggagc gcctggagcg catcaacgca accctggcag cactaccgcc
     3601 gggggcccga gaggctgccg gcatcccgtt gagacccatc gagacgctgg tgatcgcgcc
     3661 gtcccagcgg ctcgagtaca tcgctgcccg ctaccgcaac gccctgccgc cggcgctgcg
     3721 cctggtcctg cgcggtgtcg gggccacccg caggaacggg tcgatgctgc tttcctatct
     3781 gctcttcgag cggtcctata cccgcgccct ggtggaactg ggctatcgcg acgccatggc
     3841 ccggcgcggc gagatcgcgc gcttcctcgg acttgattcc gtgcaaggcc ggtcgtcgga
     3901 ttgagccctt catgcgttag aattgctagc tacgagtgca gattcagaaa aaactttaaa
     3961 ttttctgaat aagcctttat tttaaataga agtctgccgt tagaattgga actcggcggc
     4021 ggtccgtctc aggcgtgcgg ggacgtacgc cggcaggtgg gccggatctt tcggggaggc
     4081 ctcgccttcc cctcacccgg aatcagaatc gacgtaacac aatgaacatt cgcaaatcgc
     4141 tcctggcgct ggctggtgct gtcgcgctcc aggccacggg tccggcggac gccttggccg
     4201 ccacgcaccg tccggccaag aaatcttttg tggccaaggc ggcgcccgcg ccggccaaga
     4261 acgccaaggc ccgtaaccgg gccgccgccc gcgccgcttt cgcatcgccc caggtgcagc
     4321 gtaccgctgc cctcgcggtg gcgccggaga tggaccatga aggcaatccg gtcctggcgt
     4381 ccgcggcctt catggtggcg gaccaggcca ccggcacggt gctgctggaa aagaattccg
     4441 acgctgtgct gccgattgcc tccatcacca aactgatgac cgccatggtg gtgctggatg
     4501 cccgcctcaa tctgggcgaa gtcctcaccg tcgccaacga cgacgtggat accctgcggg
     4561 gcagcagttc ccatctgccg gtgggcgtgg acctgacccg tgaggacatg ctgcgcctgg
     4621 ccctgatgtc ctctgaaaac cgtgccgcat ctgcgctggc ccggcattat ccgggcggtt
     4681 tgccggcctt cgtggcggcg atgaaccgca aggcggtgtc cctgggcctc aaggacaccc
     4741 gcttttccga cagcaccggg ctgaactccg ccaacgtgtc cagcgcccgc gatctggtca
     4801 agatggtgtc cgcggcgtcc cgctatccgc tgatccgcga gttctccacc actggcaact
     4861 acaccgtgta tctcaacggc cggccacgtc agttcggtaa caccaacgca ctggtgaaga
     4921 gcccggactg gcagatcggc gtctccaaga ccggcttcat caacgagtcg ggcaaatgcc
     4981 tggtgatgca ggcctggctg ctcaataagc cgatgatcat cgtgctgctc gactccttcg
     5041 gccgcttcac ccgtgtggcg gatgcccagc gggtcaagcg ctggctcgag agcgcggcag
     5101 cccagcagcg tctggcggag gtcgtgcgcg gcggtccgac cacctgattc ggtatcccgc
     5161 cgatcaagaa aaagggctcc cgcgggagcc cttttcatgg gtgcagtgga tcagcgcccg
     5221 gccggcggga cgtagccgag ggcgcgggag atctcgtcgg ccgtggcctt gatgagaggc
     5281 gcccagtccg gattgaagcg ctcggccggg gtggacaggg acaggccggc caccaactcg
     5341 ccgctatcgt cgcggatgcc ggcggcgatg cagcgcacgc cgttttccac ctcgtccaga
     5401 tcgtaggcca cgccgtggcg gcgcaccttg tccagttcct tctcaagggc cgcgagggtg
     5461 gtgatggagg tgggcgtggt gccgggcagg ccggtgcggc gcgcgtattc cttgacctgg
     5521 tgggcgccgt cctccacaag aaagagcttg ccggtggcgg tggtatgaag gggggcacgg
     5581 gcgccgacga tgtggaccac gcgcaccgac gagcggccgc tggaggtccg ttccacatag
     5641 acgatctcgt caccggcgcg gatgcccagg ttaacgctct cgccggtttc ggcgtgcagc
     5701 cggagcatgg ccggcatggc ggtttcccgc agggagatgc gcgatttgac caggctgccc
     5761 agttccagca ggcggatgcc caggcgatag gcgccggcct cgctgcgttc gacgaaaccg
     5821 ctgccactca tggcgcccag gatgcggtgc gcggtcgagg ggtgcagacc ggtctccagg
     5881 gcgagctgct taaggggcac cggatcggag tattccgcca gcacgtcgag cagcttcatc
     5941 atgcgctcga tgacctggat gggattcttg gcggcgggtt caggcgcggt ggacaacggt
     6001 ttcggccggg atcgggaagg agcgcgcatg gtagcgcaga aggcgtcatt tcgcctaatg
     6061 aaatggcgtg gcaagcccgg tgtgcgatgc atgttccgcg ccactcaggt ggtctgcgcg
     6121 cagggggagg gctgccgggg tctcagccga ccacaaggtg tccggcgacc cgtgccagaa
     6181 tgcccactcc ggccccgagg gcggcgggct tccacacatc ggccacgcgc cggccggcag
     6241 atatcagcac ggccacgccg ggaatgtcga gaggatggat cagcaggccg gcgctggcgc
     6301 cgttgagggt ggcggcggtg gccgtgccgg cccgcagcat ctcgtccatg acgcccatca
     6361 tggccgtgcc gccggccagg tatttggtca gcgtgggcag aatcagggcc ggatcggcgc
     6421 caaccgcaag caggatcggc gcgagcaggc cggtgagcac gtcgagggcg cccaggcggc
     6481 gcagggcgag tacaacggtc agggacagga ccagcatggg gatcgcgccc accgcgatct
     6541 tgaaggcctc ggcgccggcc cggttgatga cgtccagcac gcccttggcg gattccgcca
     6601 ccgggtgatt gagcgtttcg tcgagggtgt gttcgaccgc gctcagatgc cggccgaaac
     6661 cgtaataggt ggcggcagct gcgaccagcc cgcctaccag cgaccagccc agcacgatgc
     6721 cgaagtgcag gcccatggcg gccatcggca tggacacatt ggcctgggcc atggccatca
     6781 ccatggccag ggtggccgcc agatggcggc tggacgcgcc acgctggtcc atcatcgcca
     6841 gggtggcgac gggggcagca aagctcacga aattgatctg cagcgccgcg aacacgccga
     6901 gcccggttag acccagcgga cgcagggccg gggcgagctt ttccaccagc cagtccagca
     6961 cgccgcgggc ttcgagtagg cgcatcaggg acagcatcac caccatcacg ggtagcagca
     7021 cgaagaagga cagttcgacg gcggagcggc ccgccttgag gatgacgtca atgagcaggt
     7081 ccatggttca gcggctcaag aggggggcga ggaggggcag cagccagggc agcgtgaggg
     7141 cggtgaacag gccgttgagg cccatcgcca gggcggcaaa ggcgccggtc tgctcagaca
     7201 gctggaaggc ccgcgcagtg ccgataccgt gggacgctag gccgagggcg aagcccgtgc
     7261 tggcctcgtc cttgacgccc aggcggtgca gcaggccggt ggcgaagatg gcaccgaaga
     7321 taccggtgag gatcacgaac actgcggtca gggacgccag cccgccgagc cgctcggcga
     7381 tgcccatggc gatgggcgtg gtgacggatt ttggcgccag ggagatcacc gtcggtgtgc
     7441 tggcgcccag cagggcgccg atggcgtagg ccgacaggcc ggcggtcagc gagcccacgg
     7501 cgagggcaat gaggagcggg ccgcccatcg cgacgaggcg cttgagctgg gcgtgcagcg
     7561 gtacggcgag ggcgacggtg gtgggtccga ggaggaaatg cacgaactgg gcgccgtcga
     7621 agtagtgctg gtagggcgtg ccggtggcga cgagcaggcc gaccaggatc agcaccgaca
     7681 gcagcaccgg gttgaccgcc ggatgagctc ccgccttgcg gtgcagggtc agggccacga
     7741 ggtaggcggc cagcgtgagg gagagccaca acaggggttg ggcggagaga tacacccaga
     7801 tttcggtcat cccgctgctc attgcgcgtc ttcctggcga ctgcggcggg tgagagcgtg
     7861 cagggtcagg ccggtggcgg cgaggcccag cacggtgctg gcgacgaggg ccacgaggat
     7921 cgggccggct tcgtcggcga ggcgtccggc gtggagcatc accccggtgc cggcgggcac
     7981 aaagagcagg gacaagtgct gcagcaggcc gccggaggtg gtccgcaggc catcgtccgg
     8041 accgccgcgg aagatcagat agacgagcag caacgccatg cccatcaccg ggccaggcac
     8101 cggcagatgc agggcaacac gcagggtttc cccagcaagc tggaagccca ggaggagggc
     8161 gaaggcggca atcatgggcg gtcttcctca aaaaagcggg ccgtgcggac acggcccgga
     8221 tcaaacccgc ccagggggtg gcgggcgctt acttcttggc cttggccgag gcgttgcggg
     8281 cagcctgcat gaagttgtcg aagcggttct cggccacggc gaaccattgc agctggtcgt
     8341 cgcggaattt cttccaggcc tcgtagatct tcttgaactt cggattgctg gcggcagttt
     8401 cgtcgtagag gtcgttggcg gccttgtagg cggcggccag gacgtcgttc gggaaggggc
     8461 gcagctgagt gccggaaccg accagctgct tgagcgcgcc cgggttcttg gcgtcgtagc
     8521 gggcctgcat ctcggcgtgg gcatagaggc aagcgtcttc aaggatggtc tggtattcct
     8581 tgggcagggc cgcccactgc ttcatgttca cgtacaggct gatctgcggg ccgccttccc
     8641 agaagcccgg gtagtagtag tacttggcga ccttgttgaa gcccagcttc tggtcgtcgt
     8701 aggggccgat ccattcggcg gcgtcgatgg tgcctttttc gagggccggg tagatgtcgc
     8761 cgccgggaat ctgctggggc accacgccaa gtcgggcgag gacctggccg gcgaagccgc
     8821 cgcagcggaa cttgaggccc ttgagatcct cgacgctctt gatctccttg cggaaccagc
     8881 cgcccatctg ggcgccggtg ttgccgcagg ggatgttgta gatgttgtag tccttgaaga
     8941 actcacggaa cagttccagc ccgccgccat ccatcatcca ggcggtctgc tggcggctgt
     9001 tgaggccgaa ggggatggcc gtgtcgaggg cgaaggtggg gtccttgccg acgaaatagt
     9061 aggagcaggt gtgaccgcac tcgacggtgc cgtccttcac ggcgtccatc acgccgggac
     9121 cggggacgat ctcgccggcg gcgaacacct ggatctggaa cttgccgccg gtggcggccg
     9181 ccacccgctt ggccaccaca tcggcgccgc cgaagatggt gtccaggctc ttggggaaac
     9241 tggaggccag acgccatttg atggtgggca gttcggccgc cagggccggc gcggccaccg
     9301 caccggcggc gacgccggca ctggccttct tcaggaatga acgtcgttcc actttgtctc
     9361 ctcgtgtggt gttggaatct ggatgccgat tattcatgag ccggcggcct tcccgcagta
     9421 tgggaaaacc ccaatccggc ccgccgggct tatttgcggc tgcgggcggc ggcgcgcgtc
     9481 gcctgcatga aggcgtcgaa gtggctctcg gccacggcga accactgcag ctggtcgtcg
     9541 cggaatttct tccagggctc gtagatcttg cggaagcgcg ggtttttcgc ggcggtttcg
     9601 tcgtagagag cgtgggcggc ctggtaggca gcgctcatga tctcgtggga gaaggggcgc
     9661 agctgggtgc ccgaggcgat gagctggcgc agggcggccg ggctgcgggc gtcgtagcgg
     9721 gcctgcatct ccacatgggc gagggcgcag gcgttctcca gtgcggcctg gtattccggc
     9781 ggcagggcgt tccagtgttt gagattcaca taaacggtga gctggggacc gccctcccag
     9841 aagcccggat agtagtagta cttggcgacc cgctggaaac cgagcttcag gtcgtcgtag
     9901 gggccgaccc attcggcggc gtcgatggca cctttttcga gggctgcgta gatgtcgccg
     9961 gcggggatct gctggggcac cacgccgagg cgggcgagga cctgaccggc aaagccgccg
    10021 acgcggaact tgaggccctt gaggtcttca acactgcgga tctccttgcg gaaccagccg
    10081 cccatctggg tgccggtggc gccgcagggg atgttgtgga tggcgtactc gcggaagaag
    10141 tcgcgcagca gttcgagccc gccaccgtca tgcatccacg ccatctgctg gcggctgttg
    10201 aggccgaaag ggatgcccgt gtcgagggcg aaggtgggat ccttgcccac atagtaatag
    10261 gacgcactgt ggccgatctc gacggtaccg tccttgaccg catccagcac cgccgcgctg
    10321 gggacgagtt cgccggcggc gaagacttgg atctggaagc gtccgccggt gagcagcgcc
    10381 acctgccggg ccagggattc cgtcgagctg tgaatcgaat ccaggctctt gggaaagctg
    10441 gaggcgaggc gccatttgag gatcgggagg tcggccatgg ccgccgccgc cggagccgcg
    10501 agggcgccgg ccgccatgcc ggcaccggcc tgcgtgagga atcgacgccg ttccatctcc
    10561 ggtgtctccg tcaaggcccg cagcgggtgg ggttcaggcg cccgcgacgg tcatgcggtc
    10621 cacgaggatg gagccgcagt gcttggaacc gcgcaccagc acgtccgagc cgatctcgcg
    10681 gatgccggcc agcatcgcct tgagattgcc ggcgatggtg atctcgtgca ccgggaactg
    10741 gatctgcccg ttctccaccc agaagccggc ggcgccgcgg gagtagtcgc cggtcacgta
    10801 attcacgccg tggcccagca gctcggtgac gagcaggccc cggtccatct ggcgcaccag
    10861 tccgtccagg tcgtgggcgc cgggcttgac cagcaggttg tggctgccgc ccgcattgcc
    10921 ggtggtctcg agacccagct tgcgtgctga gtaggtgctc aggaagtaac cctggagcat
    10981 gccgtccacg atgacttccc ggtcccgcgt ggccacgccg tcgtcgtcga aggcgctgct
    11041 ggcgaatgcc ttgggcaggt gcgggcgttc ggagatctgg atgtgcccgg ggaagaccgg
    11101 cttgcccagg ctgtccagca ggaagctgga cttgcggtag agggcgccgc cgcttaccgc
    11161 gtgcacgaaa gcaccgatca ggccggccgc gaggggggct tcgaagagca ccggcacctc
    11221 gcaggtggga atctggcgcg cgccgaggcg ggccagggcg cggcgtgcgg ccaaggtgcc
    11281 cacctcttcc gcgtcggcca gctcggccgg gtcgcgcttg gaggaatacc agtcgtcccg
    11341 ctgcatgtcg tcgccctcgc cggcgatcac cgagcaggac aggaagtggc gcgaactgga
    11401 gtagccgccc atgaagccca ggctgttggc cgagatgaag tgggattcct gcatggacac
    11461 gctggcgccg tcggaattgc ggatttccgg gctgaccgcg aaggccgccc gctcgcagcg
    11521 gcgggccagc tcgatggcgt cttcggagga cagcgcccac gggtggtaca ggtcgaagtc
    11581 cgggatgtcc cgcgccagca gcaccgcatc gggcagcccg gccgctgggt cgggggcggt
    11641 gaagcgggcg atggacaggg ccgccgcgac agtcgccttg agggaggcgg cagaaaagtc
    11701 ggaggtgctg gcgtagccgc gccgctgacc gtcgtacacg gtgacgccga tgcccttgtc
    11761 gcggttgtat tcgatggtgt ccacctcgtc gcgacggacc gtgacggact ggccgaagcc
    11821 ctccgacacg tccacctcgc aggccgaggc gccgcccttg cgggcgtact tgaggatgtc
    11881 gtcggccagg gtgcggagtt cgtcctgggt gaagctgaat cttttctcgg ccatggctct
    11941 cggggggtgg tacggggtca aggttataat cttaaccgtt tccagtcccc cgaaccgccc
    12001 gcaatggccc atccccattc ccatcacgac gacgacgaag acaccggccc gagcaaatcc
    12061 cagctcaaac gcgacagcca ggccctgcag gaactcggcc aggagctggc cgccctcggc
    12121 aaggagcgcc tggccaaggt gcccctggac gaagacctgc gcgacgcggt gcgtgattac
    12181 cagcgctttc cgtcccacga ggccaagcgc cgtcagttgc aatacatcgg caagctgatg
    12241 cgtcacgtgg acccggcgcc gatccgcgct gcgctggacg ccttcaaggg cgtctcggcc
    12301 gaggaaaacg cccgcatgca tcgcctggaa gccttgcgca cccgcctgct cgatgacgag
    12361 aagaccctcc acgacatcgc caccgcgcat cccggcgctg atctccagca cctccgcgtg
    12421 ctgcggcgca atgccctcaa ggagcgcgag ctgggcaagc cgccccgggc tttccgcgaa
    12481 ctgttccgcg tgctgcgtga gctcgaaggc gcacccgaca acgacccccc atccggagac
    12541 gacgaagcat gagcgagaca gcaatcatcg gcctggtgtc catcagcgac cgcgcttccg
    12601 aaggcgtgta ccaggacaaa ggcatcccgg cgctgcagga ctggcttggc tcggccctgg
    12661 ccagtccgtg gcgagcggaa acccgcctga ttcctgacga acgggcgcag atcgaggcga
    12721 ccctcatcga actgtgcgat ggggtgggct gcgacctggt cctcaccacc ggcggcaccg
    12781 gcccggccct gcgggacgtg acgccggaag cgactctggc ggtcggccac aaggagatgc
    12841 cgggcttcgg cgagcagatg cgccaggtca gcctagcctt cgtgcccacg gcgatcctgt
    12901 cgcgccaggt ggcggtgatc cgcggcaaga ccctgatcat caacctgccg ggccagccca
    12961 aggccattgc ggaaaccctc gaagggctca aggatgggga gggacggccg acagtacacg
    13021 ggatcttcgc cgccgtgcct tactgcatcg atctgatcgg cggtgcgtac atcgaaaccc
    13081 acgatgccgt gatcaaggcg ttccgtccca agtcggcgat ccggcccaag gcctgacggg
    13141 cacgcggcat cgaatccggg cggcgccggc cgtccggaat cgtccgcgag ggccccgtga
    13201 gcgacctgtc cttccagcat tccctcttcg cccgcctgcg cgcactggaa gagtcggcct
    13261 ttcccaagac ctgcgccaac tgcgggcgga aataccgcac ccacgaggaa ttcctaaccg
    13321 ccacccatcc ggtgcgccgg gatcacaccg ggctcaagca gagctacgac gacgacggcg
    13381 ccgtgatcgt ggacgtcttt cgcaactgcg tgtgtggctc aaccctgctg gaaagcttcc
    13441 acgaccgccg cgacacctcg ccggcagggc agaagcggcg cgcccgcttc gccgagatgc
    13501 tgagcagtct ggtggcggcc ggcctgtcgg aatccgtggt gcgcgccgag ttgctgctgc
    13561 tgatgcgcgg caagcccaac cggctggtgg agctgatccg ctcggtgaag gacggcgccg
    13621 ggggcggcga cggtctgccc ctgccctgag ggggtaggcg tcgattcagc ggacggccgt
    13681 gagcaggaag accgggtaat ccccctgggg tttgcgcacc acgctggcgg agtgcacctg
    13741 cacctcgcga aagccggctg ccgccgccag gccggcgatg taggcccgct cgaagccggc
    13801 gtggaagacg ccggtgtcct cgctgtggaa gctgccgtcc tccgtgtcca gatcggccag
    13861 ggcgatgaat ccgccatcgg gcagcaggcc gtggaacttg cgcagcaggg cggccacatc
    13921 cgccacgtgg tgcaaggtca tggacgagac gatgccgtcg aaggtctgcg ccagatcgtg
    13981 ggcgaccagg tcgagttcca ggatctccac cgtgcagggc aggtgcgcgc gcttggcgtc
    14041 gagctggcgg ttcatggccg gcgacacgtc cacggcggtg atgtgcgcca catgcggcgc
    14101 cagacgctcc agcagcaggc cggtgccgga gccgaagtcc agcaggcgca tggcggggtc
    14161 cagggccacg gtgtcgcgca ggacgcgggc gatggcgtcc acgttgtcta cgcggacggg
    14221 attggcgtcg aaactgtcag ccttgtgggc aaagtgatcc agcgacatgg caggcttccg
    14281 gtatgaaaat tggaagtcta gggcaaagcc gcgctgctta cttggcgacg atggggatca
    14341 ccttgccccg cgcctggggc gggggtgcat aggcgccggc cagctgacgg cggcgttcct
    14401 gctcgcgcca ccaggctctc aggccgagca ggacggcggt gatgagggcg tacagcagcg
    14461 gttcggtgat gtccttcttc accagcaccc agaaatgcac gcaggcgagg atcgcgatgg
    14521 ggtagatgct gcggtgcagg gcctgccagc gacgaccgcc caggcggcgg atggccgcgt
    14581 tgctggaggt ggcggccaac ggcgccatca acacgaaggc ggccatgccc acggtgatga
    14641 aggggcgctt gaggatgtcc ttggcgatgg cgtgccagtc gaagaactgg tccagccaca
    14701 ggtaggtgag cagatgggtg caggcatagg caaaggtgaa cagcccgagc atgcgccgca
    14761 ggcgcacgag ccagtgccag ccggtgagct tgcgcagggg ggtgacgcac agggtgatga
    14821 ggagcaggcg cagggtccat tcgccggtgg cgcgggtgag ggcctcgatg ggattggcac
    14881 ccagtccgtc caggctgaag gcgcgcccga ggaggacggc agggacgagg cacagggcga
    14941 acagggcggt cttgaggagg gcgagctggc gggtgtcggg attcatcgtg ggtcagaagt
    15001 agcgccgcag gtccatgcct gtgtagagcg aggcgacttg gtcggcgtag ccgttgaaca
    15061 tcagggtctt gcgtttgagg aattcgccga tgcggcgttc cgtcgcctgg ctccagcggg
    15121 ggtgatccac gtcgggattc acgttggcgt agaagccgta ttcgctgggg ccggccttca
    15181 tccaggcagt gagcggtggc ttgtccacca gccgcaggct gaccagggat ttggcgccct
    15241 tgaagccgta cttccagggg acgaccaggc gcaccggcgc gccattctgc accggcagca
    15301 cctctccata gaggcccacc gcgaggattg tcaggggatg catcgcttcg tccaggcgca
    15361 gaccttccga gtagggccag tcgagcacgc cgcgggtcat ggtggcgcgg tcgtaatggg
    15421 aggtgaactc cacgtacttc gcgctgccct ggggatcggc cagcttgagc acgctggcga
    15481 ggggcaggcc aacccacggg atgaccatcg accagccttc cacgcagcgc atgcggtaga
    15541 tgcgctcttc cagcggcgcg aggcggaaca ggtcttcgag ggcaaaggtg cggggcttgt
    15601 tcaccaggcc ttcgacgcgc agggtccagg gcgcgggctt gaggcggaag gtgttctcgg
    15661 cggggtcggt cttgctggtg cccagttcgt agaagttgtt gtagccggtc accgcgcgca
    15721 gggatgtggg cgtttcggtg gtactcaagg cgctgtggct gagggggctg agggggccga
    15781 gggcggcctg tccggcagcc ggcagcaggc tgcccagggc gagggcgccg gcctgaccca
    15841 tgaagcggcg gcgggccccg tatacggcgg ggtcggtgat ttcggagggc ggcaggtcgg
    15901 cggggcggcg gatcaacatg gggaaagtcc ttggacgcgt ggcatgggac aagaccggcg
    15961 accgggcgtt cagcttacag aagatccgga ctatttttgc gcgggcgggc gatcctcgcc
    16021 gagctggcgg tagaccgcgc tggcgacggg cagcagggac gccctcggga gttccccgga
    16081 cagggcgtag gcggtgtgcc ggtccaccca gtagaaggtg gacagcgccc cggcatgagc
    16141 gaagcggaag gcggtttcgc cgccgtcgtg ctcgacggtg accacataca gtgtgaggcg
    16201 cccgcccgtg ccgtcctcgt acatgaacag tgcgccaggg ccctgctccg acgggatcag
    16261 gcggccaccc accaggcgga agccttccgc cacgagatgc ggcgcccgaa ggggcgtgcc
    16321 caggcgcttg gacaaccacg ccacaagatg cgattcctgg tcggcgccga cctccaccgg
    16381 gtgacgcact tccggcgcat aaaccgcgta ggccacggct gcgcggtggg gcagcgccgc
    16441 cagcggatcg tccactgtcg ctgcgggccc gtcggggggc agcacgcgac cgatcacgat
    16501 gcccagggcc agccacccca gcacggcggc aacgcgcaac aaggggcgcg gcgtgggcgt
    16561 ggggggcgcg aggagatggg gcggcacgga ttgctccagc acgccgtcgt aggccgcgtg
    16621 cagggcagcg ttctggcggc gccagtcatg gacggcggcg gcgctgtcgg gatggtcggc
    16681 gagccagccg gccacgcgca cccgctcgtc ggcggggagc cggtcatcgg cgaaggcgtg
    16741 caggcggtcg cgatccggcg gggtgttcat ttgacgacct tcaggtgcag ggcgcgctcg
    16801 tcgcccgcca ggagcaggcg cagcttgtcg cgggcgcggg ccagacgcga catcacggtg
    16861 cccacgggca cgccgatgac gcgggcggtg tcctcgtagc tgagctcttc caggcccacc
    16921 agcaacagga cttcccgctg ctcgggcgac aggcgggcca gggcccggtc gagatcgcgc
    16981 agttccaggt tgtcctgctg acgtgggcgg accggtacgt ccaggtgggt gtcgccatcc
    17041 agggagatga ccagcacgct ttcctggagc tggttgacga aaatgttgtg catcaaggtg
    17101 aatagccacg cccgcaggtt gccgggtcgc cacaggcgcc acttgccgag ggcccgttcg
    17161 agggtgtcct ggaccaggtc gtcggcgcgg ctggcgtccc ccgtcaacgc ccgggcatag
    17221 cggcgcaggc ggggaatctc ggcgacgatg gcctggcgga aactcatggg cgcatcgggg
    17281 cgggggctta tggacgggcc acgtgccaga cgctgttaaa gccgtcgccg gtggtgtcgc
    17341 cgggcttctg gtccttgacc caggtataga ccggcttgcc cttgaaggcc cactggcggc
    17401 gaccgtcgtc acggacgatc accgaccagt cgcccgacgc cttgtcgccc tcggccgcca
    17461 cgaggggagg ccagttgttg gcgcaggggc cgttgcaggc gctcttgccg ctgccggcgg
    17521 cgtccttgtc gaacacatac agggtcatgc ccttggcgtc ggcgaggagg ccgtcgcggc
    17581 tctgggccgg tgcagtggcc agtccgggca gggtggtgca tgcggcggcg agcagggcgc
    17641 ccaggccgat cagggcgtgt gtgacggaac ggtgcatcat gggaggtctc cggtggggaa
    17701 tgggtgatgc gtaaacaacc gggcagtccg gtttattccg tcccgtgcaa aatattctgc
    17761 agcggtgtcc cgcggggcgc tccttcagtg tagtccgtct tttccgccta gcggctgcga
    17821 agccgactct gctaaactcg ccgccagttc cggcgccctg ctcggagcgt gcgggctgtg
    17881 ccggcctttc cgattcctgc ccttttccgt tccgcgacac ctgttccgcc cgatccggat
    17941 cgatacccga tgcgcattct tctcagcaac gacgacggct atttcgcgcc cggcctgcag
    18001 gccctggctt ccgccctggg ggacgtgggt gaaatcagtg tggtggcgcc ggagcgggac
    18061 cgcagcgggg cgagcaattc cctgaccctt gatcggcccc tgtccctgcg ccgcgcggcc
    18121 aacggtttcc acttcgtgaa cggcacgccg accgactgcg ttcacctggc ggtcaccggc
    18181 atgctcgaac aactgcccga catcgttgtc tccggcatca atcatggcgc caacatgggg
    18241 gatgacacga tctactcggg caccgttgcc gcggcgaccg aaggttatct cctcggcgtg
    18301 ccagcgattg ccatgtccct ggtgtcgaag gaggcgtccg atttcagcgc ggcggcgcgc
    18361 atcgctcgcg agcttgtggc gcgtttcatc cgtcagccgg tgcgtcagcc cacgctgctc
    18421 aatgtcaatg tgccggatct gccctacgac cagatcaagg gcatccgggt gacccgcctc
    18481 ggcaagcgcc acaaggccga gcccaccatc aagtcggtca cgccccgtgg cgagacggtg
    18541 tattgggtcg gtgccgccgg tgcggcccag gatgccggcg aaggcaccga tttccacgcg
    18601 gtggcccacg gctacgtgtc ggtcacgcct ctccagatcg atctcaccca cgccgggcag
    18661 attgccccgg ttcaggactg gctcgcgtca tgagcgaggt ggcccgtgtc ggtgttcacg
    18721 gcgcccagcg tgcccgtgcc cgcatggtcg agcgcctgcg cgagcagggc gtgcgcgacg
    18781 agcgggtcct ggcagccctg ctgcatgtgc cccgtcacct ttttgtcgag gaggccctgg
    18841 cctcccgcgc ctacgaggac acggccctgc ccctcgggct gcagcagacc atctcccagc
    18901 cctttgtcgt ggcgcgcatg ctcgaactgc tgctggccgg ccgcgaaagc ctgggcaaga
    18961 ccctggaagt aggcaccggc tgcggctacc aggccgcggt gatggcctcc ctggcgagtg
    19021 acgtgtattc cgtcgagcgc ctgcgcctgc ttctcgaccg cgcgcgcgcc aatctgcgcc
    19081 acctgcgcct gcccaacgtg cgcctgaaac atgcggatgg cagtgtcggt ctcaaggatg
    19141 cggccccttt cgacaccatc atcgtcgcgg ccgccgcgcc aaaggtgccg cccgccctgg
    19201 tggaacaact ggccgagggt gggcgtctga ttctgcccgt aggcatggcc gagcaggtac
    19261 tcgtgctgat cgagcgcacc gtccgcggcc ccaaggaaac ccgtctcagc ccggtacgat
    19321 tcgtcccgct gctgcccggt accgaatgaa gcaccgtgaa ggaaccatgt tgtcccgtct
    19381 gtccctgagt ctgttcctgt ctgcgctggc cctcgccggg tgtgccacca agtcccccgc
    19441 tccggtggcg gaccgggcca aggcgggtgc cacgccgccg gtccgtgccg aaacgcccgc
    19501 agcgccggca ccggtggtgg cacgccccgg ttactacatc gtcaagcccg gcgataccct
    19561 gatccgcatc gccctcgatc atggtcagaa ctaccgggac atcgccgcct ggagcggcct
    19621 cgaaaacccc aatctcatcg aagtgggcca ggaactgcgc gtcaagccgc cggagtccgg
    19681 tgcggtggtc aaaccggtgg cgcccgcggg cgccgacgtg cgacctgtgg cgccgtcagc
    19741 agacggtctg cgccgcgagc ccaagggcgg caaggtgccc tattcggagc aggcctgggc
    19801 tcaggcccag aagccggagg cacctgtcgt gccggtcaag gccgacgcca aagccgagcc
    19861 ccggacggac gccaggcccg atgccaagcc ggacgtgaag cccgagacga agccggaagc
    19921 caagcccgag acggccgacg acaagggcaa ggacgccgcc aaacccgcgg cagcgggcga
    19981 cgaccgcatc gaatggtcct ggccggccgg tggcaaggtc atggccggct tcaacgatac
    20041 ctccagcaag gggatcgacc tctccggcaa gccgggcgat cccgtctatg cggcggcggc
    20101 cggcaaggtg gtgtatgccg gtacgggttt gcgaggatat ggcaaactcc tgatcgtgaa
    20161 gcacgataac gcctatctga gcgcttatgc ccacaaccag cagttgctgg tgaaggaagg
    20221 gcagtcggtg agcaagggtc agaagatcgc ggaactgggg gacacggaca gcgaccgccc
    20281 caagctgcac ttcgagatcc gcaagcaggg caagccggtg gatcctgtga aatacctgcc
    20341 gtcccgctga gcaatgacac atccgtaaca ggtgggcgac atgatctgaa gtgttggtgc
    20401 ggcactaccc ctcgtcaaga agcggggcgt gccgggcatt gcggggcggg cggcgggaaa
    20461 ccctccggcg cccctttgac cccagaggac atccatgtac gagccgggcg tgcctgaaga
    20521 agagctggag acccccgaac cggatctgcc gccggaggtt gaggtctttc tcgagacccc
    20581 cgcgccagtg gatacggaat tccttggcga cgtcacccag ctctatctca acgagatcgg
    20641 tgccaacccg ctgctcactg cggaagagga gctgagcctc tcgcgccgcg ttcgtgaggg
    20701 cgacttcccg gcccgccaga cgatgatcga gcgcaacctg cgcctcgtgg tgaacatcgc
    20761 caagcattat ctcaaccgcg gcatcccgct cctcgacctg gtggaggagg gcaacctggg
    20821 cctcatccac gccctcgaga agttcgatcc ggagcggggc ttccgctttt ccacctatgc
    20881 cacctggtgg atccgtcaga acatcgagcg ggccatcatg aaccagtccc gcaccatccg
    20941 cctcccggtg catgtggtga aggagctcaa ccaggttctg cgcacccagc gtcagctcga
    21001 agcgtccggc catggcgaca ccaccgtcga ggaggtggcc cgccagctca gccggcccgt
    21061 ggaggatgtg cgcgccatcc tcgccctcaa cgagcacacc gcgtccctcg acgcgccgct
    21121 ggatatcgat ccaagcctct ccatcggcga atccctggcg gatgaaggcg cggaaacacc
    21181 cgacacgcag atccacggcc tggaggtcga gagcctcgtg cgcgagtgga tcggccagct
    21241 caacgaaaag cagcgcatgg tgatccgcca ccgctacggc atcgacgagt gcgaggtaca
    21301 gaccctcgaa gaactggcgg acagcctgga cctcacccgc gaacgggtgc gtcagatcca
    21361 gctcgaagcc ctcggccagc tgcgccgcat catcaagcgc cggggcgttt ccaaggacgt
    21421 cctgttctga acacccgatc catcatgacc gacacgatcc gcctctacca caacgcccgc
    21481 tgttccaaga gccgctccgc ctgcgccctg ctggccgaca agggcgtgcc gaccacgatc
    21541 atcgagtacc tcaagacgcc gcccacccgc gaagagctgg ctgccgtgct ggcggcgctc
    21601 ggcatgcggg ccgaagacct ggtgcgcaag ggtgaggatg tcttcaaggc gaccttcaag
    21661 ggccggaccc tcagcgagga cgaatggctg gacgcccttg tcgcccaccc catcctcatc
    21721 gagcggccca tcgcagtgcg cggcgcgcga gccgtggtgg gacgcccgcc tgaaaaggtg
    21781 ctggaactgc tctgaggcac gtccgaccgt cgcaacggtc ggacggccgg ttccgggcgg
    21841 gatgcgcggg cctcagcggc gcgcctgacg ggcttccttg acggcctcgg ccagctggaa
    21901 caccgacatg gcgtagaagc tgctgcggtt gtagcgggtg atcacgtaga aattctgata
    21961 gcccagccag tattcgctgt cggcgccggg ggtgaccagt tgcacgaagg cggccggccc
    22021 gaccgcgggc gttccggagg cggcgtgcac gccgtgggcg gccagggcct cggcgtcgaa
    22081 ggccggcgtg atgtcgttgg cgatcagcgt ttccagcgtc gcgtcggcat tcgtgaccgc
    22141 ccggatggcc accggcccgc cccgttccca tccgtgcatc tgcaggtagc gggcgacgct
    22201 gccaatggcg tccttcgggc tggccagcag gtcgatgtgg ccatcgccgt caaaatccac
    22261 cgcgtaattg cgcacgctgc tcggcaggaa ctggggcagg cccagggcgc ccgcgtagga
    22321 accctggtag tcgagggggt ctcggccctg ttcgcgggcc atcagcagca gggactccag
    22381 ctcaccgcgg aacagctcgg cccggcgggg atagtcgaag gccagcgtgg acagggccga
    22441 gacggcctgg aagctgccgg tattgcgccc gtagatcgtt tccacgccga tgatgccgac
    22501 gatgatctcc tccggcacgc cgtacttgtc actggcggcc ttgattgtct ccgcgtaggc
    22561 gtcccaaaaa cgcacgccgg ccttgatgcg caccggctcg atgaagcggc cgcggtaacg
    22621 ctgccaggag cgcacgccgg gatccttggg cggcgagatg gccttgatca cggcgggctg
    22681 gaaagtggca cgacgaaaga tgaaggtgag ggcatccgga tcgaagccgt gacgttcctt
    22741 catttcggcg atgaattgct ggacgtcggg acggtccgcg tagcgttcgg cggcggccag
    22801 cggcccggcg gcgagggcgc tggccagcag gagtgcggga cggaggcgga aggggcggag
    22861 aatcatcggg ggcggaagcg tcgggcggaa tggaggaatc ggtcgcgacg ggcggcggtg
    22921 tctgacgcct cgccgtagcg caggcgcgca aaatctgtag cgagccctgc gatggcgtcg
    22981 gccagatcgg ggcgggtggt gcccacgcgg gccgcgtagg ccgtcgggcc ttcccagtcc
    23041 gcgcggggca gaccgtgggc ggcgaggcgg ctgcagacgc cttcccaggc acgcaacacc
    23101 gggtcgcggc ggcggtttcg caacagcacc ccggcgagtg cggcggtgac ggccgcgcag
    23161 gcggcggtca tcagcccggt gagctgaggc cagtccacct cgcccaggcc cagaccggcg
    23221 aggaggtcgc gctggcgggc gggattgtac ccgagaaccc attggttcca gcggtggttg
    23281 agggcgtcca ggtggtgctg gagtccctgc agccaggcca gctccgggcg catcagcagg
    23341 ggcaggtgcc cggtgtccgg gagggcggcg gcgaggccgc gctcgatgcg cgccggggcg
    23401 ctggcggcgg tggggtcgac ccgcagccag ccgcgcccgg cgagccacac ttccgcccag
    23461 gcgtgggcgt cggactggcg gacttcaaga ctgccgtcca ccgggttgat ctcgccgccc
    23521 tgataaccgg tgaccacccg cgccggcacg ccggcggcgc gcatcagcac cacgaaggcg
    23581 gcggcgaagt gttcgcagaa gccttggcgg gtgtcgaaca ggaaggcgtc cacgtcgtcc
    23641 tcacccaggg ggggcggctc cagcgtgtag gtgaggcggg cgtcgcgcat gagggcaatg
    23701 ccgcgcgcgg ggatgtcgtc cgcccggacg gcgttggccc gcagctggct gcctgcggcg
    23761 acggtgcgcg gattggcgcc ggccggcagg gccagggcca ggcgtcgcag gatggggggc
    23821 tcgtcgagac cgaccggcgt gtctgggtag gacgccaggg tctggcgcag gcggctgcgg
    23881 accggctcac gggcgaggag ctggaagtct gcgccgtagc gcaggcccgc ctgggcatct
    23941 ggaaagtcca gggccagcag ccacggccgg tcatggggtt ccagcgtcag ggtgtaggtg
    24001 taggcgcgtc cggaaacggc gtacgcgggc acgtcccggg cgggcaggtg ggcggcgcgc
    24061 caggtgtgac cgtcgaagcg ggtcagcact gggccgcgcc agtagcgctg ggcgcggggc
    24121 ggcggcgggc cgtcgaaggt ggcccggaag gcgatcgcgc cggattcact cagacgtgca
    24181 agggcacccg gcgtcatcgt gtcggagagg ccggtcagtg cgctgaaggc gtccgccggg
    24241 agcccccaca gcggcccttg gacgcgcgga aagaggatga acagcacggc catgaagggc
    24301 aggctcagga cggtgaggcg ggcggcttct gtcaggcggg ggcgcagggg ccgggcgcgt
    24361 ccggcgaggg ccgccagcgt ggccagggag acgaccgtgc cgaccagggc cagggcggcg
    24421 gtggccatgc cctgctggtg caggaactgg cccatctgca ggaaaaaggt gagcaggatc
    24481 acggcgcgcc cgccgcgaat gtcgtccgtc tccagcagct tgaggcccag gaaggcggcc
    24541 agcagggcga tgcccggatc cttgccgaac aggtggccgt attcgaccaa caccccgccc
    24601 gcgacggcga ggccgagggt tccgagcagc gcccggtggg gcaggcgcgc gcgctgcgcg
    24661 gcgagcatgc cgcgccatga caggaacagc gcgcagacgg cggacagcca gtgggggagc
    24721 tgcgcggcgt ggggcagcag gctgacgccg gctgcgacga cgagccagcc ggtctggtcc
    24781 ggacgcaggc cacgctcagc catcagggcc tccgaacagg gccagcgcgg tgaggcaggc
    24841 aaggcgatgg gcgggaccac tggccggcgg aaaggtttgc tgaggcatgc gcaggccgta
    24901 ctccagtccc gcggcctccg catcgcacag ccagcgggtc aggcgggcga ggcgctgctc
    24961 cgtgtccaga ccggggggca gggtgttcca gtccagccag agcgcctccg cgcccccgcc
    25021 gtcaaactgc ttggtgagcc attcgccgct gcgggcaagg gctttccagg ccacgtgacg
    25081 gggcgggtcg gcgggctggt gggcgcgcag gccggcgaag tcgtcacgcc cttggcgtct
    25141 gccgtgggcg cccggccgca gacccgtgcc gcgcggcaag ggcggcgggt cggcttcggg
    25201 cgtcgggaag accagggcgc gaagatcggg ctggaacagg ctccaggcgc ggatgagacc
    25261 cagcggatga cggctctcca gctggaggcg ggggaggggc agccagccgc gttggggggc
    25321 cgccacgtgg acgcttatac gcacctggct gtcgggcggg agctcgaagg cttcgtccgg
    25381 ggacgcgccc gtgcgggctg gccacaggcg cagggcgaga cgggaacgtg gcagcgggtt
    25441 ggtcatgagg acgccaaaac tggccgtggc gccggcaaag accggctcga cccgtcccgg
    25501 cgctattgac agtcccacta gattccgaaa gctcaggtgg atgtggaccc agcccacgcc
    25561 gcccagcagg aaggtaaggg cgtagcccag gctcagggcg tagttgatgg aggcgaggag
    25621 catcaccagc agggtcacgc ccagcgccac gccaaaggcg gtgggcatca cgaagatccg
    25681 gcgctggccc aggcggatcg gcgccggctc cgggccgtgg acgcggaaca gccagcgctg
    25741 gaagcgggcc cgcagtgcgt caacgcgcgc gtggaccggc atcagggcag aggaacggct
    25801 tcgaggaggc ggcggacccg ttcttcaggc gatgtgcggc ggtccccgtc actgcgcagg
    25861 cggtggccgg ccaccgccgg gaggacggcc tgtacgtcct cgggctgcac gtggtcacgt
    25921 ccggccagca gcgcccaggc ccgcgccgag gccaccagac ccaggccggc gcggggcgac
    25981 aggcccaggc tgaagccggt ggcctggcgg ctgtgggcga gcagggcctg gacatagtcg
    26041 agcagcgggg gggccacatg gacgcgggct gcggcgtcga acagggtgtc cagctcgccc
    26101 ggcgcgagca gtgtctcctg gcgggccagc agcgcgcggc gatcctcgcc ggccagcagg
    26161 gcccgctctg cggccgggtc ggggtagccg agggcgatgc acatcaggaa gcgatcgagc
    26221 tggctctcgg gcagcggaaa ggtgccgatc tggtcggccg ggttctgggt ggcgatgacg
    26281 aagaaggggg ccggcagggg cagcgtgcgg ccctcggcgc tgacctggcc ttcctccatg
    26341 gcttcgagca gggcgctctg ggccttgggg gtggcccggt tgatctcgtc ggcgaggatc
    26401 atctgcgcga agaccgggcc cggatggaag cggaaggccc cgtcgcgggg gtcgaacacc
    26461 gacacgccga ggatgtcggc cggcagcagg tcactggtga actggatgcg ctggaactgg
    26521 agccccagca ggcgggcaag gaggtgggcc agggtggtct tgccgacgcc cggaacatcc
    26581 tcgatcagga ggtggccgcg ggccagcagg caggccaggg cgaggcggat ggcctgctcc
    26641 ttgccgagga gaatccggcc ggcggcatcc acgaggcgat tggcgatgta aaggggcatg
    26701 gcgtgggctt ttgcagggcg tgggttatgt ggataatggg gatcaccagt gcattcccgg
    26761 tgctgcgggt ggtctccagg agatggagcc gcatggggct gtgaagttcg ccgggaagac
    26821 gatcaatcaa tcaaacagac gttttcgcga gtagaggggg agagtgtatg acgagtaccg
    26881 cattcatcac gcatcgggag tgctggctgc acaacatggg cgcccatcat ccggaatccc
    26941 cggagcgtct cgcagccatc aacgaccgcc tgatcgcggc ggggctggac atgtacctct
    27001 cgttctacga cgcgccggtg gccaccgccg aacagctcag ccgcgcccat ccccgcgagc
    27061 acgtggacgc aatccttgcc agcgcgccgg agcatggcat ccgtcacctg gatccggata
    27121 cggctgtgtg cccgcactcc atcaaggcgg ccctgcgttc ggccggtgcc ggcgtgatgg
    27181 cgaccgatct ggtgatgtcc ggcgaggtgg agaacgcctt ctgcgcggtc cgcccgcccg
    27241 gtcaccacgc cgaagcggcc aaggccatgg gcttctgctt cttcaacaat gtggccgttg
    27301 cggcccgcca tgccctcgat gtgcatggac tcaagcgcgt ggcggtggtg gacttcgacg
    27361 tgcaccacgg caatggcacc gaggacatct tcaaggacga tccgcgggtg ctgatggtca
    27421 gcatcttcca gcatccgttc tacccctaca gcggacatga caatcccgcg cccaacatgg
    27481 tcaatgtgcc cgtcaaggcg ggttcccgcg gcgacgtgtt ccgcgagatc gtcaccaaca
    27541 tctgggtacc ggccctgcgc aaccacgcgc cggaggcgat cttcatctcg gcgggtttcg
    27601 acgcccacta cgaggacgac atgggctccc tcgggctggt ggagaaggat tacatcttcg
    27661 cgaccgaaga gctcaagaag gtcgcggccg agttctccaa gcggcgcatc gtctccatgc
    27721 tggaaggcgg ctattccctg tcttccctgg cgcgcagcgt tgtggcgcac gtcaagtcat
    27781 tggctgacct ctgagtagcg gagcgcccgg ccggagggtc cgatccggcc gggcgttccg
    27841 ctgcccgcgc ttctggcatc cggttcgcgc tgcgcagacg ggcttttggt aagcgcattt
    27901 ttcgcccact ggccttcccc aagtgattgg gtacaatagt tcactttccc tttcccgcga
    27961 tagctcatga ttaccggttc gattgtcgcc atcgtcaccc ccatgaacga cgatggcagc
    28021 ctggatcttc cccgtctgcg cacgctgatc gacttccatg tccaggaagg aacggacggc
    28081 atcgtcgtgg tcggcacgac cggtgagtcg cccactgttg atgtggacga gcactgcgaa
    28141 ctgatccgtg tctgcgtgga acatgcggcg ggccgcattc cggtcattgc tggcacgggg
    28201 gccaattcga cccgagaggc catcgaactg accgagttct cccgcaaggc gggcgctgtc
    28261 gcgggcctgt ccgtggtgcc ctattacaac cggccgaccc aggaagggct gtaccgccat
    28321 ttccgggcca tcgccgaggc gagcgagctg ccgctgatcc tctacaacgt gccgggccgg
    28381 accgtggctg atctggccaa tgacacggcc ctgcggcttg ccgagattcc caacatcatc
    28441 ggcatcaagg atgcgaccgg cagtctggac cgcgcctgcg atctgatcgc ccgggcaccc
    28501 agggatttcg ccctgtacag tggcgacgac atgaccatga tggccttcat cctgctgggt
    28561 gggcacggca cgatttcggt gaccgccaac gtggcgccgg ggctgatgca tgaggtgtgt
    28621 gccgcggcgc tccagggcga cgccgtcacg gcccgtgcgg ccaacgccaa gctggtcggt
    28681 ctgcaccgcg acctgttctg cgaagccaac ccgattcccg tgaagtgggc tgtccagcag
    28741 atggggctga tcggtccggg catccgcctg cccctcactc ggctgtcaga atcctgccac
    28801 gagcgtgtcc gcaaggccat gcgccaggcc ggcatcgaag tttgagttcc accatcccct
    28861 cccgaggctt acgcatgcat agccgcaccc tggcgtccgg atcggcggtt gcgatggcga
    28921 tcgccctcgc cggctgctcc ggttccctgc tggaatcgaa gaagatcgac tacaagagcg
    28981 cgtccgctgc gccggtcaag tccctcgaaa taccgccgga tctcaccgcc ccgacgcgcg
    29041 atgaccgtta cacggtgccc gatgtcagtc ccaagggaac cgccacgttc tcggcctaca
    29101 gtgccgaacg cacgggccag ggcgcacagg tgggcgccac gcctggcgca gcgccgaccg
    29161 cggcatctgc gtcggggtcc gacaagatgc atatcgagcg tgccggttcc cagcgctggc
    29221 tggtggtcca gggttcagcc gacaagctgt ggccgcagat ccgcgatttc tggcaggaaa
    29281 gcggtttcct cctgaacgtg gacaagcctg aaatcggcgt aatggaaacc gactgggccg
    29341 agaaccgggc gaagatcgag cagggcttca tccgcaatac gcttggcaag gtgctcgatg
    29401 gcctgtattc cacaccggag cgcgacaagt tccgcacccg tctggaagcc ggtgccgaag
    29461 cgggcacggt ggagatctac atcagccacc ggggcatgat ggaaatctat cctacggaag
    29521 gcaaggaccg cacgatctgg cagccccgag cggccgatcc ggaacttgaa gcggagttcc
    29581 tgcagcgcct gatggtccgc ctcggtgcgg acgaaagccg agccaaggcc caactggcca
    29641 ctgcgccgtc ttcggacaag gccaggctgg ccaatgcggc ggatggggca acgctgctga
    29701 ccgtgcagga tgccttcgac cgcgcctggc gccgtgtcgg gctggcactg gatcgggtcg
    29761 gtttcaccgt cgaggaccgg gatcgctccc agggtctgta ctacgtgcgc tatgtcgacc
    29821 ctgaagtgga cgtcaaaaag aagggtgacg agggcttgct ctccaaactg gccttctggc
    29881 gcagcgacaa gccggcactg ccgacgggca gtcagtaccg catcttcgtc aagaatgcgg
    29941 gagacgaatc gtcggttcag gtcctcaccc gcgaaggggg tgtcgacaaa tcggattcgg
    30001 cgcgcaagat tctgggcttg ctgtacgagc agctcaagta aggcctgcgc acccgtcacg
    30061 gatgaacgcc gacctgaggg tcggcgtttt tcttgaagcg gcggctgcgg tatccgatcc
    30121 caggcttgcc gattcaggac aggcgcgatg ggcggacggt cctgcgggga ggtgcctcgc
    30181 ggtgtggcag agggatggag accgcgatgt gctccatcaa tgggtacaaa aaaagccaac
    30241 ccgcaggttg gcttttttcg ttgccggaag aaggattact tcttgtcggc agccggagcg
    30301 gcgggagcag cttcagcggg cttggcgtca gcagccgggg cagcgggagc ggccggggca
    30361 gcggcttcag caggcttggc gtcagcagcc ggggcagccg gggccggagc agcagcggga
    30421 gccggagcag cggcaggagc cggcgcttca acggccttgg gttcttcctt cttgccgcaa
    30481 gcggaaacgg ccagggcgac gagagcagca acgaggagag actgtttcat tgttggtttc
    30541 ccttatcgat aagtgagttc aacggcctga aaatcaggag caatcaggtg ggggacccga
    30601 tctgagagga ttctagcacg ccgggtgtgg ccccagaaag acattgttgc gttgccgcaa
    30661 catcgatgga atgtcgcggt agatgccaag tgcttgaagt tatttgcttt cgatgctgtc
    30721 cgggtgggca aagccgcagc agtaccagtc gtacagaagg gcctgtccat cctgcgtcag
    30781 atcggcaacc gtggagatcc tgcgccggtc tgccagttcg atcatgacgg gcagatccgt
    30841 gtgggagatg ctcacggctt caccattgag aaagaagcgg ccgtccgaga acagaagcag
    30901 gctgcgcgcg tccagcacat agcccgccct gttgcactgg gcgagaaagc tgtcgtgatc
    30961 gagtggttcg tctggcgagt cgaagaacac atgaggcttg ggttcggtca ggtaggcgcc
    31021 gaggaaacgg cgcacgtcct cccgtgtcca cgtgatccgc gcgagctgtt cggccacccg
    31081 atccaccatg gcatcgccga tctcggcgga gtggacgtga ggcttcaggt cagggtcggc
    31141 gtaaaggcct tcgagctgca cggcatcctg gagatagacc aggaactcct gggccagctc
    31201 ctggtaggtg ggcgaacgga agccgatgga atacgtggtg cattctccaa gggcaatgcc
    31261 gttgtgggcc cagtggggcg gcaggtagag catgtcgccg gattccagca cccagtcgtg
    31321 caccggccgg aaatcctgca ggatcttgag ggggacatcc tcgatgaggc tgcggtcgtc
    31381 ctggttgctg atgagccagc gccgctgacc gatgccctga agaaggaaca cgtcgtagtt
    31441 gtcaaagtga ggtccgacgc caccgccatc gacggcgtag ctcaccatca gatcgtcgag
    31501 gcgggcctgg gggatgaagt cgaaacgatg gagcagggcg tcgccttcgg gcacgaagag
    31561 gttgagtccc tgcacgagca ccgtccacgg ttgcttcttg cggcgcaggt cggtggcttt
    31621 ttgcgggccg tggaggactt cccagcgggc accgtcgtgg gcgatgaagc gcgattccac
    31681 atctggatcg cgggcgaggg cgaagaggtc gtccctggag accacgccgg tgaagccggg
    31741 gatggcctga cggatgagga ggggcttttt ctgccagtat tcggcgagga actgctcgac
    31801 cgaaaggcca cccagaagtg tgtcgatcat gccgtgatta tacgcccttg ccttgcaaca
    31861 gccggctctg tcgctagaat ggcgaccttt cttttcactc ttgcgcccat gcatgtcgac
    31921 gccccccgcg aagcgctgat cgaatccctg gaccacgatg ggcgcggtgt cgcgcgggtg
    31981 gacgggaaga cggtcttcat cgaaggcgca ctgcccggtg agcgggtgga gttcgtctcc
    32041 taccgggtca agcccaagtt cgaattggca gacgtgacgc gcatcgtggc acccagcgcc
    32101 caacgcgtgg caccgccctg cgtcctgttc ggtgagtgcg gcgggtgcag catgcagcac
    32161 cttgaaccct ctgcgcagct ggcggccaag cagcgggtgc tggagaacgc cctgtggcat
    32221 ctggcgcggg tgaaggctga cgtgctgtat ccggtcattt cgggcgcgcc atggggttac
    32281 cgcttccgcg cgcgtctgag cgtgcgccag gtgcctagca agggcggcgt gcggatcggc
    32341 ttccatcaac ggcgcagcag tcacattgtg gatatgcgca gctgtcccgt gttgccgacc
    32401 catgtgtcgg tcctgctgcc ggccctgcgg gatctggtga cggccctgtc ggtggcggat
    32461 cggctgccgc agatcgaggt ggcggtgggc gagggcctca cggtgctggt attccgccat
    32521 ctggaacccc tgaccgcggc ggacaaggac cacctgcggg cgtttgccga atcccacggc
    32581 gtgcaggtgt ggctgcagcc cttcgggccg gattcggcct ctcgcctgca cccggtcaag
    32641 gcgcccggac tggcctacac gctgccggag ttcggcgtct ccatggcttt ccggccgacg
    32701 gatttcaccc aggtcaacat gggcatcaac cgcctgctga tgcgccgcgc catgctcttg
    32761 ctggaccccc ggccgggtga gcgcattgcg gacctgttct gtgggctggg caacttcagt
    32821 ctgcccatcg cccgcctggg ggccacagtg gtcggggtgg aaggcagcga cgcgctggtg
    32881 cggcgcgccg ctgagaacgc ggcggccaac ggcttggcgg aacgcagcga attccatgcg
    32941 gccaacctgt tcgaagccac cgaagacagt ctcgcggccc tcggtcggct cgacaagctg
    33001 ctcatcgatc caccgcggga aggggctatt gcggtagtga aggcgctcgg cgcggacagt
    33061 ccgcggcgcg tcgtgtacgt gtcctgcaat cccgcgaccc tcgcgcggga tgcggcggtg
    33121 ctggtgcacg agaaaggcta ccggctgaag ggggcgggca tcgccaacat gtttccgcag
    33181 acctcccacg tggagtccat cgccctgttc gagcgggatt agccgaggca cgggtcagca
    33241 aaaaggcgac ccgcgggtcg cctttttgcc ggacggggtg ggatcagtcc cggtcgccac
    33301 cgaaggccat gagcaggttg agcagattga cgaacacgtt atagacgtcc agatagaccc
    33361 gcagggtggc cgtcacatag ttggtttcgc cgccgcgcac gatgtcgttg atgtcgtaca
    33421 ggatgtaggc ggagaacagc gccaccgcga tcgccgagat cgtcaggtag agggccggca
    33481 cctggaaaaa gatgttggcc acactggcca gcagcaggac gatcacgccg acgaacagga
    33541 acttgccgag gccgctgaaa tcccgcttgg agaccgtggc cacggtggcc atcacgccga
    33601 agatcgccgc cgtgccgccg cccgccaggg cgatcagcgt gccgccgttg gagaagccga
    33661 gggccacccg cagcaggctg gatagcatca gccccatgaa gaaggtgaag cccagcagca
    33721 gcgccacgcc caggccactg ttcttgttgc gctcgatgcc ccagaagaag gcgaaggaga
    33781 tgcccatgaa cagcaggaaa cccatcagcg ggctgccggc aaagaacgag aattgcatct
    33841 gcacgccaag catggcgcca gcgatggtcg gcaccatgga cagtgcaagc agcaggtatg
    33901 tattgcgcag cacgcggttc gtactggcgc caagcatctg cgtctgctgg ccgtaagtgt
    33961 cgatttgcat agatttctcc attgaattga gagggttaag gtcgccgccc gttgaggggc
    34021 tgtactgcct gagagtatcg gacgtgcggg aaagtttcaa ccgtgcaggg agaacgcgcc
    34081 tggggggccg tgctagaatt cgggccggcc tgacggcatc ccgccgtcga tcagggcctt
    34141 ccggaagcgt ggcagagtgg tcgattgcac cggtcttgaa a
//
LOCUS       DAOQPA010000026        33483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_567_length_33483_cov_16.803219, whole genome shotgun sequence.
ACCESSION   DAOQPA010000026 DAOQPA010000000
VERSION     DAOQPA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 33483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33483
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_33483_cov_16.803219"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="PKX01_05675"
     CDS             <1..1698
                     /locus_tag="PKX01_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ75647.1"
                     /translation="NLIGLDAITGATVTVIAQNQVMMTSGTAVAQQVGILKPVIRPAA
                     RFTASTATPTWADLVADGSVQRLTVQPEDVGLKGDGRPYMDLWFGYLNQPTLGRALLG
                     ADGYASLMSRLKDGEHALFIIKTAGMDSFKGSGFVRGGLYDRVQVRQDKDAFTFRDLD
                     YINLWGLSAAGAPAYDESAIFIIRGKGFSAAYPWKLVFLGNKIDRETGAKTFANFDRE
                     YWLDARYLEGGRPLVVKPDPTWLKVWKDKALDIGLFVALLTIIATAYARRDALVRRAT
                     HRNKWPVNAFKYTGWVVSIGFVGFHAMAQPSITQVLTWFHALLFQWKWELFLSDPLIF
                     LFWIFIIATTFLWGRGFFCGWLCPFGSLSELLYKLAGAVGLKRLQRHPSPAFHARAKW
                     LKYGLFFGLLAVSFFSMSLAEQLAEVEPFKTTFLVGLLNRSWPYTLFAGGLLGLSIFV
                     ERPFCKYLCPLGAALAMPTTFRWFGLKRKMECTQCKACAKGCGSLAIDPKGNIDAREC
                     MLCLDCMVLYYDDHACPPLAQERKRRGKAGQALTPIGSDGYFIPIHPAPLPRQGETP"
     gene            1695..2114
                     /locus_tag="PKX01_05680"
     CDS             1695..2114
                     /locus_tag="PKX01_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription regulator"
                     /protein_id="HMZ75648.1"
                     /translation="MTVADPTELRQPPYGDTRGVARLSAEIIDHLWPWRRGSGGSGLA
                     RGAGITLALAATVAWILTALGHLSAGAIIGWWFGWSVLEVRVRHDAKPYVKDGPWWGR
                     RYRRANLMDLICYVSFKNLLIGAALFIGLKTAGLLVV"
     gene            complement(2199..5006)
                     /locus_tag="PKX01_05685"
     CDS             complement(2199..5006)
                     /locus_tag="PKX01_05685"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HMZ75649.1"
                     /translation="MVKLKLRRVCAAVGAGLGGVCAASAIAAAVPETVTLNAVADSYV
                     DANSYNNYGKIVYFMVGSTKSAYLRFDLSSLADRDIRSATMKVRAQKPFSDTFSVMHA
                     VDNGWNEYSLGWFNRPYNVVSAQGALSTVATTNGGWATVDVTNAVSLTQGRPLTLQIS
                     GTNTTPLWFDTRESGNTPTLTVTSVPKSVTLTPVQDSYVDPAMPDTTFAGAPSMKADP
                     SGAEVIMRFDLSALTDRTVLGGTLHLKTPYGGPQDVQIYMPANADLQPNVLTYTTQPT
                     LLPDPVSAPAVAEPGGVAIDVGSILKGLNVRSPLSLVIRSSGSTLGIASMETATPPTL
                     EVLYRSVSITSTPLSSVPLKVGINIEATNYYGTEVPGIDLMKQGHTWYTQCYPWRDAL
                     CSAGKFSRSGGSSWDTLEQDVLDQDASGWVRSLPAPGDGATTGVNYTSIATLIPSSLS
                     DRYPGGRYIVRYDGEGTFSLTNSKATVTRNTALSSPGRAVFDIVGNPATAVGSAMNLS
                     ITATDPNRTGNYLRNIRIFPPGGVCSDDVTRYCNAQTGVPACSTGAICQDFETVYPAK
                     PFDPRFLANIKRFDTLRFMGYQSTNNAVNEAEWSDRTRPDHYTWIRTGYHLNPIENVV
                     DLGNTLHRNIWVNVPHRASDDYIYQLATAVKNRLSGDLKVYVEYGNEIWNTMFSAGSW
                     VEQQGLAEWPYAPDSAYVKRLQWQGKRTAETCDIWASVWGSDKSRVICVAGIQTGSAW
                     AAGYVLDCPLYAAAHGGIPCYQHNMKAVAVAPYFGGYIGDWSHKAAVESWTLDADGGL
                     GRLFAELFQGGQFTDSPPGGAIASALRALDTSVGLSRDRGLQTVSYEGGQHLTAVGAS
                     LQSPAIVQLLTRANRDARMGDAYKTYLAGWVGSGASLFTQWHSVGSYGVWGNWGLLEY
                     RDQPGSPKFNAVQTFLGTVGQ"
     gene            complement(5315..5521)
                     /locus_tag="PKX01_05690"
     CDS             complement(5315..5521)
                     /locus_tag="PKX01_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMZ75650.1"
                     /translation="MATGIVKWFNDAKGFGFITPDGGGEDLFAHFSAIQAKGFKTLKE
                     GQRVEFDVATGPKGLQAANIRPAD"
     gene            5766..7088
                     /locus_tag="PKX01_05695"
     CDS             5766..7088
                     /locus_tag="PKX01_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMZ75651.1"
                     /translation="MSITPHACMFAPFFRHVRHAAACAATVLTALSGPGAPSAHAADW
                     RVQPGDSIQAAIQRAAPGDTVRVARGRYVENLRIDKPLNLLGEGRPTVSGGLRGDTLR
                     IAAPDVRVDGFIVTDSGADLGEQNAGIYIQPGAHRARVSHCDFSYTLFGLWIEKANDV
                     VIEHNLITGKRDLDSSQRGNGIQLYNTTGAQILANNISFVRDAIYVDVSHRAVFRGNR
                     MHHSRYGTHYMNSYHNLWEDNDTYFNRGGLALMEVRDQIVRNNRAWGNSDHGIMLRTI
                     QDAIVENNVVAGNARGFFIYDAEYNTLRGNLVVDNQVGVHLWAGSVRNIVERNDFISN
                     REQVKYVAARDETWGAKDGNHWSNYVGWDRNGDGFGDVPYEANDLVDRLSWRHPLVKL
                     LLASPAIQTLRLIAQQFPLLRAPSIVDPHPRMRPDHPDWRHWLGKHYR"
     gene            7069..7983
                     /locus_tag="PKX01_05700"
     CDS             7069..7983
                     /locus_tag="PKX01_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75652.1"
                     /translation="MASTIVETRGVTRHFGTVQAVQGVDLAVQAGELFGLIGHNGAGK
                     STLFKMMLGLLPVTAGEILIDGRPVNGPDFRDTRRRIGYLPENVVLYDNLTGLETLHF
                     FADLKAVPRRDCAPLLDTVGLAPAAGRRVREYSKGMRQRLGFAQALLGTPRLLFLDEP
                     TTGLDPAAIREFYRILKDLREQGVTMILTSHILAEIQERVDRLAILEDGRVRAVGTVQ
                     ALREAMDLPVTLEIAGGAGLREQVARILAGLPVERLDETHDSLHIRVPREAKMPALAR
                     LATLGETLRDLHVREPSLEDVFFGYRGQ"
     gene            8021..8842
                     /locus_tag="PKX01_05705"
     CDS             8021..8842
                     /locus_tag="PKX01_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ75653.1"
                     /translation="MSIDFRQIGTIAGKEFWDRIRNRWVLAVALVFTVFALVIAYFGA
                     AQQGAVGFRSIDVTIASLVSLVIYLIPLIALILGFDAIVGERERGSLDLLLTLPITRL
                     ELLLGKYAGLAAALAFSTVAGFGAVGLILSPQLDLNALFHYGGFMLSAILLGFAFLSL
                     AVMLSVFAADRTRASGLAIGTWFFFVLVFDLLLLGALVVTAGDYGGSVFPYLLLLNPA
                     DVFRVLNVFSLDEVRTLYGLATVFPEALSHPAVLGSVMFAWIVAPLALALWRFQR"
     gene            8839..9372
                     /locus_tag="PKX01_05710"
     CDS             8839..9372
                     /locus_tag="PKX01_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMZ75654.1"
                     /translation="MNIPRPLRRLGLAAGLGACVLLAACGDAARSGGATPVEITRDTY
                     CALDGMLLADYPGPKAQIHYDGQPEPEFFCDTTEMFAIYLKPEQARKVRAVFVQDLDK
                     TRWDAPQGAWIDARTAYYVVGSGKRGAMGPTIPSFGSEAGARKFAEAQGGQVHPFAAI
                     TPDMARLDGGALHDQKM"
     gene            9392..9661
                     /locus_tag="PKX01_05715"
     CDS             9392..9661
                     /locus_tag="PKX01_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75655.1"
                     /translation="MEPFYANLPPELALDVEQRARLLYALRGARDEVLARLGAGDADQ
                     ALACLRRGELPEHPAYDDILSARILADLHAQVRSDLATLLADSQR"
     gene            9672..10043
                     /locus_tag="PKX01_05720"
     CDS             9672..10043
                     /locus_tag="PKX01_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HMZ75656.1"
                     /translation="MSLHPDLAARLDERFGTRFDAPVELKLDALLLRLDNGVILEARI
                     AAPDAYAIAWVWGDAELRIDTAPVHPELATFPHHVHGADGRLRADPLTVCGADPFDNL
                     VRVVDALLEDPLLGAPPGTAP"
     gene            10040..10534
                     /locus_tag="PKX01_05725"
     CDS             10040..10534
                     /locus_tag="PKX01_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMZ75657.1"
                     /translation="MIRSPFAALGIAAFLAILPASAADPAPQPVPRNARCAVCGMYPA
                     QYPQWKAQIVFNDQSAAQFDSPAELFRFLHNMPAFDRRHSAADVGAVWVSDYASKAWV
                     DARKAVFVAGSRARGPMNDANLPAFSTREAADAHIRSQGGRALAYEEITREVVHHLAG
                     GHAH"
     gene            10737..11375
                     /locus_tag="PKX01_05730"
     CDS             10737..11375
                     /locus_tag="PKX01_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75658.1"
                     /translation="MPATTVSASNVIADPTKTLQRFDLLDLEQYTQEKSYATNASKDF
                     HLFYVGRDDVHEILKHILSRVSVSLYMNMFGFDDEELNDILMQLANDPSVVMMITLDK
                     SQTGGVHEKRLLDSDAAKNPHAFNTYFVIGESATHQISHTKGFVADGRVGGEGSTNWS
                     ASGEGTFVVQGHAGGPGYKAQNNTQTIFTDPDTLSRFQAELIAEHLAARGKA"
     gene            11397..11885
                     /locus_tag="PKX01_05735"
     CDS             11397..11885
                     /locus_tag="PKX01_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75659.1"
                     /translation="MKPQRTLVLLLSVVALSATAAVVNGGGYVAGDGFTLSEALAQAR
                     QEHPPGSFRPFWLLALDGAVGALARRGAGPDMATAVADIQRRGGTVYVCEQDLRAAGL
                     NSAELLPGVQAVRGWSDAESGIRNEDGASDAGADDAEQPLSPLRAVNRLCGSRPTPDD
                     SR"
     gene            complement(11877..12707)
                     /locus_tag="PKX01_05740"
     CDS             complement(11877..12707)
                     /locus_tag="PKX01_05740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ75660.1"
                     /translation="MDPIARTAFYCCGVRAADAASPRPICGDHLAARFMDDEARAVFA
                     RFARFRGPNLSNAARHRLVDDFLRAHLARHPDGLIVLLGAGFDTRAFRLPGGRWVELD
                     QPALLARKEHALPADTAPSPLQRVGIDFATQDLAGVLAPWAGHETAVVVLEGVSMYLT
                     DAAFAATARALHAVLPGHTLLCDLMDARFARRYGGGLRRAIRALGGDFAPDRADPAAF
                     VESLGYASRSVASIPARAVEHGTLGIPRWLFNTLLRSLRDGYRLHGFEARRSPASPSA
                     "
     gene            complement(12868..13275)
                     /locus_tag="PKX01_05745"
     CDS             complement(12868..13275)
                     /locus_tag="PKX01_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ75661.1"
                     /translation="MTAIRILPVLLVALLAACGKSEAPKTEAPPPAPAAAAPAPQAAA
                     PAADTADGGKGAELFKKTCAMCHQTGVAGAPKLGDKADWGPRIAQGKDTLYKHAIEGF
                     NGNKGAMPAKGGNPAFADDEIKTIVDFMVSKAE"
     gene            complement(13410..15623)
                     /locus_tag="PKX01_05750"
     CDS             complement(13410..15623)
                     /locus_tag="PKX01_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ75662.1"
                     /translation="MKTRLRPLPAALMLMTLLPAALSARAAADSVELAPVVITASRSE
                     TRVEEMPLHTTVIAREDIERSPARSLDQLLRSVPGMNFTGVPASQSDPTGHQTRMRGL
                     GNAKVLVLLDGVPIHDPFYMTTQWFKVPLSNVERVEIVRGGNSSLWGNMAVAGVVNIV
                     TRRVQDDAGEVSLSAGSYGTWNVALSKNVAVSEALGFNLTVDDYQSNGYQTTPAEFMW
                     RFPHKQPVAVENRNVQLTAYFRPSADLNGWLRLGYHIQDQDISYQYGSNVQKSPDLAG
                     SLTRRFDDKTRLTASAWAQQVKFEKYNGSTCYYQGGTSCLNSNSATLTPAKVNDQVVE
                     FYTQYGSQRYREQGTSVVFSQDLAGRFSSYQLGVDYRALRAEDAEWFYTTPTSPTAPQ
                     GNLNSTTWGKGRQTFAGLFGQGKFFPVDPLEVTLSARYDTWRIDERANLRTTAAGVAS
                     GGALQDGSKNAFNPSLALRWELSDALSLRSAVYRAFRAPGFNNLTRTFGTGTSTTIAN
                     PDLAPETLTGWEAGADFRRGPFNLGATWFHYNIKNMIATYTATAASAPAQVQVICGGP
                     TLPGCGGSAKYYTNDQDGRAYGIELVAGWQVRPTLSVDASYTHTETFLTRHGSIVTDP
                     LNVQLVATPRDVAAVGTTWQPVERFSTYTELRYIGSMYLDTTSNGATARLQQGAIPLV
                     NASVRYAWDRRTDLFVSLVNLLDREYSENAYTINQPYNRTLSMPRTATAGVTLRF"
     gene            15924..17105
                     /locus_tag="PKX01_05755"
     CDS             15924..17105
                     /locus_tag="PKX01_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HMZ75663.1"
                     /translation="MKKILPILFPLLAALTLPAAAHEGHDQGPAARPARIELATAAAF
                     GPDGQLHIVAKDGAHLVWRHSPDLGATWSAPQHVNMQAESIAADGDSQPRIAFNRDGE
                     LLVTWTKPLPKPYTGEIRLARLPRDGSAFDAPITVHRDQAEITHRFQNLIVAPDGRVT
                     VVWIDKRDQEAARLTQKPWRGAGVYSAISDDGGRSFQPERKLAEHSCECCRIALALDP
                     DGTPVALWRHVFAPNERDHALARLKADGTPEAVQRATFDRWRVDACPHQGPALAIAAD
                     GVRHAVWFNQRDGEGRVFYGRPTSAGVNGQRPVGGPAAAHADIAVLGRRVVLLWKEFD
                     GERTRLRAQRSDDDGRHFDPPQELDATAGASDQPRLLTRDGRAYAFWRTADAFRVVPL
                     P"
     gene            17102..17590
                     /locus_tag="PKX01_05760"
     CDS             17102..17590
                     /locus_tag="PKX01_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75664.1"
                     /translation="MIRATLALRAALAALFIAAAPLPGHAATELQPLDRHAARSLTDP
                     RSHTRPTAIALWSAECEHCKKNLAALAELARRHPQLRIVTVEAEPATADSAPLLARTG
                     LTRDERYAYGDESPEALAHALDPRWRGELPRTYLFDGQGGSLTRSGVLTAADMRSALK
                     LK"
     gene            complement(17637..18002)
                     /locus_tag="PKX01_05765"
     CDS             complement(17637..18002)
                     /locus_tag="PKX01_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ75665.1"
                     /translation="MKREHAEPVPQPPEGCPLERYLHVVSGAWVPRILWFLSFGPRRF
                     GELRRDIGNISAKVLTAKLRDLEGAGLVARTETRGQTRQVAYALTERGRIFEPVFDAM
                     VEVSHRLAELEAREAASKD"
     gene            18192..19784
                     /locus_tag="PKX01_05770"
     CDS             18192..19784
                     /locus_tag="PKX01_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMZ75666.1"
                     /translation="MHSTLRLTLLTLALATGGSAVASDGPTTADFNAAADDTSNWLYA
                     THDYSGQRYSRLRQINRSNAARLQAVCMYRSADAGPAQTNPVVYDGTMYLSIARSIVA
                     IDAATCREKWKYTWEPKGNELSPTNRGVAIKDGKVIRGTADGYLIAVNARDGSLAWSR
                     QIASSTGQQYLSMPPLIFEDMVIYGPAGADFGQKGWVGAFNLSDGTERWRLPFIPDPG
                     QPGAETWSNPEALKYGGGSLWTPVTLDAKAGTLYVAVGNPAPDFYGDARPGNNLFTNS
                     LLAVDVRSGKMLWYHQAVPHDIRDWDLTHAGPLFSTTVEGVRRNVIAASGKDGLLRLI
                     DRDSHEVLYTVAFTTQENVDVPLTVEGTRSCPGLLGGQEWNGSAYSPRTNTLFVPAVD
                     WCTTFKKAATTPEFKREEHYYGGSIVMDPFDKARGWLTALDASTGKVRWRYASGTPML
                     AAVTATAGDVLFTGDFNRDFLALDATSGKVLYRFNTGGEVAGGIVTYAVGGRQYVAAV
                     SGYVSAFFKGSGPAGVTVFALR"
     gene            complement(19721..20896)
                     /gene="ypfH"
                     /locus_tag="PKX01_05775"
     CDS             complement(19721..20896)
                     /gene="ypfH"
                     /locus_tag="PKX01_05775"
                     /inference="COORDINATES: protein motif:HMM:NF008525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HMZ75667.1"
                     /translation="MLPSSSIPVALPAHADRLVLLFHGVGSQPAAMVPLGERIARALP
                     AAAVVAVAGPQPSGTAGGREWFSVAGVTDANRPGRVAEALPTLLDAVAEWQRRTGVAA
                     EATVLVGFSQGAIMALATLARADAPARTVVAIAGRLAEPPAQVAPATTVHVLHGEADP
                     VMPIGLAEAAVARLAQLGARVSFDRFPGLGHGISEALAAGLVARLLGDGAPAGEVGSV
                     SALAIRLEAEQAIVSRPAAQGGEEVRSLALGPATLVRRCLHHTPPTAGELEAAIDLIE
                     PQIMALGLHLPAGAVLRLSGDWPADLQRPAPGLHRDAVEGLFSRLAAIAHGRPAASEG
                     LPADPAYFAAALLLRELLHHLGAVRAEAAAPAQGGGPQRSANTVTPAGPEPLKKADT"
     gene            21146..22030
                     /locus_tag="PKX01_05780"
     CDS             21146..22030
                     /locus_tag="PKX01_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HMZ75668.1"
                     /translation="MARPILEHTMALPRPAPVLRLAALLPLAVLCGPTLAPAQDAAPC
                     SPARLAAIERQSQPLDRSGHGPDRGSAEWASAVEWRLGLRGRDDLPARGSRAWCERIE
                     AALRQRDEQARLYASRPGPAFSCRGVQPGSIPDLVCADTALGTLDRDLQRVYAQAART
                     AAHERPPVLKAEQRGWVRGRDDCWKSDDRRACVADAYVTRIAELQARYRLVAHRGPFR
                     FSCNGQPADEVLITHFQTDPPTLIAERGDQTSLMFLQPAASGTRYVGRNESFWEHQGE
                     ATVVWGHGAPEMRCILDR"
     gene            complement(22075..24567)
                     /locus_tag="PKX01_05785"
     CDS             complement(22075..24567)
                     /locus_tag="PKX01_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HMZ75669.1"
                     /translation="MNGALLNPEEVRVTDATVTGGKGRQLAQLRHFGLPVPDFAVLPA
                     AWSEGRGEAFPAALVTALHDGLAARGWLEQPLAVRSSAVGEDSAAASFAGIYTSCLNV
                     QGLDGVVAAVQTVWASLDSDTARAYRARMGHAGPARMGVIVMPLLAAEASGIGFTCDP
                     LTGREDRLVIHANRGLGETLVGGETAGDEYVFAEDVEDAWRLIECRPGPKTCMSVPAP
                     GGGTRRVALPDAAAAPLFDTERAEALAALLRDAAVAMDFVAPFYDLEWVWDGARFWLT
                     QARPVTRRPRHAYGPLQAQPAIWTRGNTCEVMPEPLQPADWNFSRRGVNDLLVQGYVL
                     AGFPVLAGAQRAGLFEGRLYLEASLLQWEAWDAIGMKPERLNALMGGHQPAIDVTPPG
                     GRERLRRALTTLRYLVRAPALRRRGEAEIAAMHALARQVRQGPPPRDRDALRRLLYTV
                     SRPGRTSIGAFFLQGSGGASLSFLVERLEHFFPGEGEALGAALLTGGTPSVTAQQGYD
                     LLRLARLARAAGFPAQVPAQGDYARAFAEFMEVHGHRGHYETYMHSASWRERPERLLE
                     QLPALAEVDEAALRARQQDAAAAAWQRVRREVPFWHRPLLRSLVKAARRECNQREAAR
                     SGLIALLEVCRMAWRLAADLLRADGALAERDDVFFVLPSEAERAVDGLVPPAGLRARV
                     AARKALYEAWQAVTPPEYLCIAPAGEWLAAPAWPAPPHRPGEGWRGVATGTGRARGRA
                     RILRHPSEGSRLQAGEILVAPSTDPGWTPLFLKAAGLVVETGGYMSHGAIVAREFALP
                     AVVNLPGILDGLQDGDEVEVDGRSGEVRLIRP"
     gene            complement(24564..25175)
                     /locus_tag="PKX01_05790"
     CDS             complement(24564..25175)
                     /locus_tag="PKX01_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ75670.1"
                     /translation="MSTPPRSDPGRRERKRLLQLDHLADTAWALFDAEGYEAVTMERI
                     AAVADVAKGTLYKHFPVKEALLRHRFHRELREGLERLQPVVLAEAPGAARLRRFFDLS
                     AQWSERHRETLLPYVRFRLAEPCHGDDSQRSGLAQVFTGLIADGQGAGGFRTDIGAEV
                     LANYLEFLYLASLLRWLTGATPVLQAEFERMLAVFLGGLEVRS"
     gene            complement(25510..26439)
                     /locus_tag="PKX01_05795"
     CDS             complement(25510..26439)
                     /locus_tag="PKX01_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75671.1"
                     /translation="MSPSPACAVPAAFYLDVSDGHRLFVATAGAPGGEPVLVLHGGPG
                     SGCSPRLAELYDPARHWLVMPDQRGAGRSAPAGACHANTTAHLIADLERLRRHLGIAR
                     WRVTGGSWGATLALLYAAAHPAAVSGVVARSVFLPGAGNVAAFFAAGGDVPAERVALA
                     DAAGCPAGGDVLAALARRLDDPEPARAVRAAVAWAAYERALAEPEAPPQADLDAAAAE
                     CLVRKYRVQAHYLQAGAFVTEAGFLDACASLPGVPVTLIHGRLDRVCPPDNARRVHAR
                     IPGSRLVWVDGCGHAAFHPGMVAAWRQALGGAG"
     gene            26785..27702
                     /gene="pqqB"
                     /locus_tag="PKX01_05800"
     CDS             26785..27702
                     /gene="pqqB"
                     /locus_tag="PKX01_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HMZ75672.1"
                     /translation="MRVHVLGAAAGGGFPQWNCNCRNCRGLRDGSLSARRRTQSSIAV
                     SADGARWTLVNASPDILQQFQAFAGTWPTDGLRGCGVESIVLVDAQIDHTTGLYMLRE
                     KGSPWPVWTTDPVYEDLTRGNPILHLLDHYCGVDRRAIPLDGRVFRPQGAEALEWRAM
                     PLLSAAPPYSPHRGQPVPGDNIGLTIRDPASGRRVFYAPGLAEVDDAVFDAMAGADCV
                     LVDGTFWTDDEMIRLGLSTKRARDIGHLAQSGPGGMIEQLARLPRHVRRVLIHINNTN
                     PILDEDSPERATLTAAGIEVACDGMEIAL"
     gene            27699..28421
                     /gene="pqqC"
                     /locus_tag="PKX01_05805"
     CDS             27699..28421
                     /gene="pqqC"
                     /locus_tag="PKX01_05805"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HMZ75673.1"
                     /translation="MNTPVKPAPWTPAEFEAQLRDKGAAYHIHHPFNVLLNTGGARPE
                     QIRGWVANRFYYQIIIPQKDAAILANCPDRGVRREWIQRIHDHDGHGDDAGGIEAWLR
                     LGQAVGLARDEITDLRHVLPAVRFACDAYLNFCRQRPWQEAVCASLTELFAPTIHKQR
                     LANWPEHYPWIESDGLQYFRNRVTQARRDVEQGLAITLAYFDTRAAQQRALDILKFKL
                     DILWTMNDAMAQHYGLAGGRPA"
     gene            28442..28726
                     /gene="pqqD"
                     /locus_tag="PKX01_05810"
     CDS             28442..28726
                     /gene="pqqD"
                     /locus_tag="PKX01_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HMZ75674.1"
                     /translation="MHSTPAPSSHPRIGRLFRFQWEPAQQAHVLLYPEGMVKLNRSAG
                     EILARCDGTRSVAEIVAELEAAFQASSLTADVEAFLRLAEERSWIDWTPA"
     gene            28737..29861
                     /gene="pqqE"
                     /locus_tag="PKX01_05815"
     CDS             28737..29861
                     /gene="pqqE"
                     /locus_tag="PKX01_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HMZ75675.1"
                     /translation="MEALLIPPRPGPLWLLAEVTYRCPLHCPFCYNPVDFAQAGPELS
                     TNDWLHVIRQARALGAAQFGLSGGEPLLRDDIEILVAEARRLGFYTNLITSGVGLTEA
                     RLAALKDAGLDHIQLSFQDATREANDLITNTRTFDLKRRVAALTKAAGYPTVINCVLH
                     RGNIEHLDRIIEMAAEIGAEYLELANTQYHGWAYLNRAALMPTAAQLAEAERVTERWR
                     VRLGKAMRILFVTPDYASTAPKKCMNGWGEVFLAITPDGLALPCHTARMLPGLDFPDV
                     RAHDLAWIWHDSPLFNRYRGTAWLPDPCRSCPDKEADHGGCRCQAFMLTGNPDATDPV
                     CPKSPDHALIQTALQAAEQPTAQPLVFRDPKQSAALARPF"
     gene            complement(29956..31569)
                     /locus_tag="PKX01_05820"
     CDS             complement(29956..31569)
                     /locus_tag="PKX01_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75676.1"
                     /translation="MTIVKRLLLLVGAALISLLAMTAVNYVQMNRVYDAANFGNINVV
                     PSIEVLNKVAVEFGRLRVRVYRHVMNTDEKAMQEVEATIMAGREHVRKFFKDYEPLIA
                     DDEDRRLLEAERASFDRYNKNIDEVLVASRANRNDEARALMAKFAPDAVHFIEQLEAH
                     MKFNEDLGHKMAAEGAAARDSATWFAVGILVVACAAMGVISLMTIRAIAGRLEEANAL
                     AARVAAGDLTGGKASPNSGSDEIGQLLASLDAMRVDLAQTISGIATNAENVVGYAAQL
                     SSAAQQVSVSTEQQSRNTAAAAAAVEELTVSIDHVGSSAGDARVRAADAGSKAVDSAR
                     GVDTAADKVGQVASQVETTSQQIQTLSQQVQQIDKITTVIREVADQTNLLALNAAIEA
                     ARAGEQGRGFAVVADEVRKLAERTTSSVQEISNVVGAIQQGAVGAVNSMQVSRDLVAA
                     VVGSARTASESMSEIRESASTMSGVIESISDALQEQRAASTELARNVESIAQMSEENA
                     AAVGSVAETAHRLSSVSDALKSNVARFRF"
     gene            31805..32287
                     /locus_tag="PKX01_05825"
     CDS             31805..32287
                     /locus_tag="PKX01_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ75677.1"
                     /translation="MKARARLASDLPDGVVIAPLDDGPGPLREAAAAILLASFDDPER
                     YGPARIQSQLDSAPLPYYRRFFIARRDDEVLGVGGIKAADWASDTHLLYLSAVAEHAR
                     GQGIARALVMTRVAWVMETFEHGRVLVSTPRPRRFARFGFRAAGRLESAGRRLMLLEF
                     "
     gene            32391..>33483
                     /locus_tag="PKX01_05830"
     CDS             32391..>33483
                     /locus_tag="PKX01_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMZ75678.1"
                     /translation="MSAAHEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEIR
                     ARKAARHATSDWMELEKQRGISVTSSVMQFPYRDCVINLLDTPGHEDFSEDTYRTLTA
                     VDSAVMVIDSVNGVEAQTIKLLNVCRLRDTPIVTFINKLDREGREPIELLDEIESVLG
                     IQCAPMTWPIGMGKRFRGVYHLYNDTVQFFDPQAEKGTSEIIEGLDNPRLDALIGDQA
                     DELRLDIELVRGASHSFDRDAYLGGKQCPVFFGSAVNNFGVQSLLDAVVDLSPAPLAR
                     PAKTRQVQPNEDKFSGFVFKIQANMDPKHRDRIAFIRVCAGRFERGGKLKQVSTGKSL
                     AINNAITFMAQDRNTTDEAWAGDIIGIPNH"
BASE COUNT         5375 a        12689 c        10377 g         5042 t
ORIGIN      
        1 aacctcatcg gcctggacgc catcaccggc gccaccgtca ccgtcatcgc ccagaaccag
       61 gtgatgatga ccagcggcac cgccgtcgcc cagcaggtgg gcatcctcaa gccggtgatc
      121 cgcccggcgg cgcgcttcac tgcctccacc gccacgccca cctgggccga cctggtggcc
      181 gacggcagcg tgcagcgcct caccgtgcaa cccgaggacg tgggcctcaa gggcgacggc
      241 cggccttaca tggatctgtg gttcggttac ctcaaccagc ccaccctggg ccgcgccttg
      301 ctcggcgccg acggctacgc cagcctgatg agccgcctca aggacggcga gcacgcgctc
      361 ttcatcatca agactgcggg catggattcc ttcaaaggat cgggcttcgt ccgcggcggc
      421 ctctacgacc gggtgcaggt gcgccaggac aaggacgcct tcaccttccg cgacctggac
      481 tacatcaacc tgtggggcct gtctgcggcc ggtgcgccgg cctacgacga atcggcgatc
      541 ttcatcatcc gcggcaaggg cttctccgcc gcctatccgt ggaagctggt gttcctgggt
      601 aacaagatcg accgggagac cggcgccaag accttcgcca acttcgaccg ggaatactgg
      661 ctcgacgccc gctacctgga aggcggccgc ccgctggtgg tcaagcccga ccccacctgg
      721 ctcaaggtgt ggaaggacaa agccctcgac atcggcctct tcgtcgccct gctcacgatc
      781 atcgccaccg cctacgcccg ccgcgacgcc ctcgtacgcc gcgccaccca tcgcaacaag
      841 tggccggtga atgccttcaa gtacaccggc tgggtggtca gcatcggttt cgtcggcttt
      901 cacgccatgg cccagccgtc catcacccag gtgctcacct ggttccatgc cctgctgttc
      961 caatggaagt gggagctgtt cctgtccgat ccgctgatct tcctgttctg gatcttcatc
     1021 atcgccacca ccttcctgtg ggggcggggc ttcttctgcg gctggctgtg ccccttcggg
     1081 tcgctgtccg agctgctcta caagctcgcc ggcgcggtcg gcctgaagcg cctgcagcgc
     1141 cacccgtcgc cggccttcca tgccagggcc aaatggctca agtacggcct cttcttcggc
     1201 ctgctcgcgg tgtccttctt cagcatgagc ctggccgaac agctggccga ggtggaaccc
     1261 ttcaaaacca ccttcctggt cggcctgctc aaccgcagct ggccctacac cctgttcgcc
     1321 ggcggcctgc tgggcctgtc gatcttcgtc gaacggccct tctgcaagta cctgtgcccc
     1381 ctcggcgccg cgctggccat gcccaccacc ttccgctggt tcggactcaa gcggaagatg
     1441 gaatgcaccc agtgcaaggc ctgcgccaag ggctgcggct ccctcgccat cgaccccaag
     1501 ggcaacatcg acgcccgcga atgcatgctg tgcctggatt gcatggtgct gtactacgac
     1561 gaccacgcct gcccgcccct ggcccaggaa cgcaagcgcc gcggcaaggc cggccaggcc
     1621 ctcacgccca tcggcagcga cggctacttc atccccattc accctgcacc gctcccccgt
     1681 caaggagaaa ccccatgacc gtcgccgacc ccacggaact gcgtcagccg ccctacggcg
     1741 acacccgcgg cgtagcccgc ctgagcgccg agatcatcga ccatctgtgg ccgtggcgac
     1801 gcggcagcgg tggcagcggc ctggcgcgcg gcgccggcat caccctggcc ctcgccgcca
     1861 cggtggcctg gatcctcacg gccctggggc acctttccgc cggagccatc atcggctggt
     1921 ggtttggctg gagcgtgctc gaagtgcgcg tccgtcacga tgccaagccc tacgtgaagg
     1981 acggcccgtg gtggggacgg cgctaccgcc gcgccaatct gatggatctg atctgctacg
     2041 tgagcttcaa gaacctgctc atcggcgccg ccctgttcat cggcctgaag accgccgggc
     2101 tgctcgtcgt gtgagacgaa cgcccgtcgc acgccgtgtc accgtgaatc ccccacgcca
     2161 cctgtccgca gctgacggac cggtggcagg acaaccccct actgcccgac cgtccccaga
     2221 aatgtctgga cggcgttgaa tttgggcgac cccggctgat cccggtactc cagcagcccc
     2281 cagttacccc acacgccgta gctgccgacg ctgtgccact gcgtgaacag cgaagcacca
     2341 cttcccaccc agccggcgag ataggttttg taggcatcgc ccatccgggc atcacggttc
     2401 gcgcgggtca gcaactggac gatcgcagga ctctgcaatg acgcgcccac cgcggtgaga
     2461 tgctgaccgc cttcgtagga caccgtctgc agaccccggt cacgcgacag ccccacactg
     2521 gtgtcgagcg cccgcagcgc cgaagcgatg gcaccgcctg gaggactgtc ggtgaattgc
     2581 ccgccctgga agagttcggc aaacaggcgc ccgagaccgc cgtcggcatc gagcgtccaa
     2641 ctttccacgg ccgccttgtg actccaatcc ccaatgtagc cgccgaaata cggtgcgacc
     2701 gcaaccgctt tcatattgtg ctggtagcac gggattccgc cgtgtgcggc agcatagagc
     2761 ggacagtcaa gtacgtatcc ggccgcccag gcactccctg tctggatgcc ggcgacgcag
     2821 atcacccggc tcttgtcgga accccacacg ctggcccaga tgtcgcacgt ctcggccgtc
     2881 cgcttgccct gccattgcag ccgtttcacg tacgcggaat cgggtgcgta gggccattcc
     2941 gccagcccct gctgctcgac ccagcttccc gccgagaaca tcgtgttcca gatctcgttg
     3001 ccgtactcca cgtagacctt caggtcgcca ctcaaccggt tcttgaccgc cgtcgccagt
     3061 tggtagatgt aatcgtcgct ggcgcgatgg ggcacattga cccagatgtt gcggtgcagg
     3121 gtgttgccca gatccacgac attctcgatc ggattgaggt gatagcccgt gcgaatccac
     3181 gtgtaatggt ccggacgcgt ccgatcgctc cactccgctt cgttcaccgc attgttggtg
     3241 ctctggtaac ccatgaagcg cagcgtgtcg aagcgcttga tgtttgccag gaaacgcggg
     3301 tcgaaaggct tcgctggata gaccgtctcg aaatcctggc agatggcccc cgtggaacac
     3361 gccggtaccc ccgtctgggc gttgcaatag cgcgtcacgt cgtcgctgca cacccctccg
     3421 ggcggaaaga tccggatatt gcgcaggtaa ttgccggtcc gattgggatc cgtcgccgtg
     3481 atactgagat tcatggcgga cccaaccgcc gtcgccggat taccgacaat gtcgaagacc
     3541 gcccgcccgg gcgaggacag cgcggtatta cgcgtcacgg tcgccttgct gttggtcagg
     3601 ctgaacgtac cctccccatc gtagcgaacg atgtagcgcc caccgggata acgatcgctc
     3661 agactgctcg gaatcagggt ggcgatggac gtgtaattga cgccggtcgt agcgccgtca
     3721 ccgggcgccg gaagactgcg cacccagcca ctcgcgtcct ggtccaggac atcctgctcc
     3781 agggtatccc acgaagaacc accgctacgg ctgaattttc ccgcgctgca cagggcatcg
     3841 cgccacggat aacactgcgt gtaccacgtg tgaccctgtt tcatcaggtc gatgcccggc
     3901 acttcggtgc cgtaataatt ggtcgcttcg atgttgatgc ccaccttgag gggcaccgat
     3961 gacagtggcg ttgacgtgat gctcacgctt cggtatagga cctccagcgt cggaggcgtg
     4021 gccgtctcca ttgacgcaat ccccagtgtg gaacctgacg accggatcac cagcgacagc
     4081 ggcgaacgta cattcaagcc cttgaggatc gaacccacgt cgatcgccac gcccccgggc
     4141 tcggccacag ccggcgccga caccggatcg gggagcaggg tcggctgcgt ggtataggtc
     4201 aggacgttgg gctggaggtc ggcgttggcc ggcatgtaga tctgtacatc ctgcggccct
     4261 ccataagggg tcttcagatg gagggttccc cccagcacgg tccggtcggt cagcgccgag
     4321 agatcgaaac gcatgatcac ctcggcgccg ctgggatccg ccttcatcga cggcgctccg
     4381 gcgaatgtcg tgtccggcat cgcaggatcg acatagctgt cctggaccgg ggtcagggtc
     4441 acggatttcg gaacgctggt caccgtcagg gtcggcgtgt tgccggactc ccgcgtatcg
     4501 aaccacagcg gcgtcgtgtt cgtcccgctg atctgcaagg tcagcggacg tccctgggtc
     4561 agactgaccg cgttggtcac atccaccgtc gcccaccccc cgttggtcgt agccacggta
     4621 ctgagtgcgc cttgtgcgga gactacgttg taaggacggt tgaaccagcc gagcgagtat
     4681 tcattccagc cgttgtccac cgcatgcatc acgctgaagg tgtcagagaa cggcttctgt
     4741 gcccgaacct tcatcgtcgc actgcggatg tctcgatccg caaggctgct gagatcaaag
     4801 cggagatacg ccgatttcgt gctccccacc atgaagtaca cgatcttgcc gtagttgttg
     4861 tagctgttgg catcgacata actgtcggcg accgcattca gcgtcacagt ttccggcact
     4921 gccgctgcga tggccgatgc cgcgcagacg cccccgagcc cggcgcccac cgccgcgcac
     4981 acccgcctca acttaagctt caccatgccc tgcatcctcc gcaaaagaat ctgacatccc
     5041 ctgcgtcaat ccaggggctt cacaaacccg ccgacgcaag gaacgctgga ttccgatggc
     5101 atggcgcagt ccacattttc gcggctgcac tatatatccg attcagataa cccgggacgt
     5161 ggtcttttat tcatatcttc atatgaatac agggcctcga ccgtaggcca aaaaacgaaa
     5221 tccccaccgg gcggcaatgc cgggtgggga tttcgtgtgt ccgcagggcc ctagcccgtc
     5281 acggacgccg cccggctcag gccgggggca gcgctcagtc agccgggcgg atgttcgcag
     5341 cctgcagacc cttggggccg gtcgccacgt cgaactcgac gcgctggcct tccttcaggg
     5401 tcttgaagcc cttcgcctgg atggcggaga aatgcgcgaa cagatcttca ccgccgccat
     5461 cgggcgtgat gaaaccgaaa cccttggcgt cgttgaacca cttgacaata ccggtagcca
     5521 tgtcttgaac ctcgaaaaaa atgttgaacc gcgaaactca agacagccgg tgatggaggt
     5581 gccgcaaatc ctgcaaacta ccgtacaaca acgtcacatc ctgaagctcg ctggggcagc
     5641 actctgcacg ggtttttgcg accttgcaac gactattttt tcattgcact gcaacaatcg
     5701 ggcctgcttc agttcaaagt tcatccacat caaggcaggg ccctccggaa acagcccata
     5761 ctgggttgtc catcacgccc cacgcctgca tgttcgcccc attcttccgc cacgttcgtc
     5821 atgccgccgc ctgcgcggcc actgtattga cggcattgag cggccccggc gccccgtcgg
     5881 cccacgccgc cgactggcgc gtgcagccgg gcgactccat ccaggcggcg atccagcgcg
     5941 ccgcccccgg cgacacggtg cgcgtggccc gcggccgcta tgtggagaac ctgcgcatcg
     6001 acaagccctt gaacctcctc ggcgaaggcc gtcccacggt gtccggcggc ctgcgcggcg
     6061 acacgctgcg gatcgccgcg ccggacgtgc gcgtcgacgg cttcatcgtc accgactccg
     6121 gtgccgacct gggcgagcag aacgccggca tctacatcca gcccggtgcg caccgggcgc
     6181 gggtgagcca ctgcgacttc agctacaccc tcttcggcct gtggatcgaa aaggccaacg
     6241 acgtggtgat cgagcacaac ctcattaccg gcaagcgcga cctggattcg tcccagcgcg
     6301 gcaacggcat ccagctctac aacaccaccg gcgcgcagat cctggccaac aacatcagct
     6361 tcgtgcgcga cgccatctac gtggatgtct cccaccgggc ggtcttccgc ggcaaccgca
     6421 tgcaccacag ccgctacggc acccactaca tgaactccta ccacaacctg tgggaggaca
     6481 acgacaccta cttcaaccgc ggcggcctgg cgctgatgga ggtgcgcgac cagatcgtgc
     6541 gcaacaaccg cgcctggggc aattccgacc acggcatcat gctgcgcacc atccaggacg
     6601 ccatcgtcga gaacaacgtg gtcgccggca acgcccgcgg tttcttcatc tacgacgccg
     6661 aatacaacac cctgcggggc aacctggtgg tggacaacca ggtcggcgtg catctgtggg
     6721 ccggctcggt gcgcaacatc gtcgagcgca acgacttcat ctccaaccgg gaacaggtca
     6781 agtacgtagc cgcccgggac gagacctggg gcgcgaagga cggcaaccac tggagcaact
     6841 acgtgggctg ggaccgcaat ggcgacggct tcggcgacgt gccctacgag gccaacgacc
     6901 tggtggaccg cctgtcctgg cgtcaccccc ttgtcaagct gctcctcgcc agcccggcga
     6961 tccagaccct gcgcctgatc gcccagcagt tcccgctgct gcgcgcgccc agcatcgttg
     7021 acccccaccc gcgcatgcgc cccgaccacc ccgactggag acactggctt ggcaagcact
     7081 atcgttgaaa cccgcggcgt cacccggcac ttcggcaccg tgcaggccgt ccagggcgtc
     7141 gacctcgccg tgcaggcagg cgaactgttc gggctcatcg gccacaacgg tgccggcaag
     7201 agcaccctct tcaagatgat gctgggtctg ctccccgtca ccgccgggga gatcctcatc
     7261 gacggccgcc cggtgaacgg cccggacttc cgcgacaccc gccggcgcat cggctacctg
     7321 cccgagaacg tcgtcctcta cgacaacctc accggccttg agaccctgca tttcttcgcc
     7381 gacctgaagg ccgtgccgcg ccgcgactgc gcaccgctgc tcgacaccgt cggcctcgcc
     7441 cccgccgccg ggcggcgcgt ccgcgagtac tccaagggca tgcgccagcg tctcggcttc
     7501 gcccaggccc tgctcggcac gccacgcctg ctcttcctcg acgagcccac caccggcctc
     7561 gaccccgccg ccatccgcga gttctaccgc atcctcaagg acctgcgcga acagggcgtg
     7621 accatgatcc tcacctccca catcctggcc gagatccagg aacgggtgga tcgcctcgcc
     7681 atcctcgaag acggccgcgt gcgcgccgtc ggcaccgtgc aggccctgcg cgaggccatg
     7741 gacctgcccg tcaccctgga gatcgccggt ggcgccggcc tgcgcgaaca ggtcgcccgc
     7801 atcctcgccg gcctgcccgt cgagcgcctc gacgagaccc acgacagcct gcacatccgc
     7861 gtgccccgcg aggccaagat gcccgccctc gcccgcctcg ccaccctggg cgagacccta
     7921 cgcgacctcc acgtgcgcga accgtccctc gaagacgtgt tcttcggcta ccgcggccaa
     7981 tgaacacccg ccgctccgta acctgcctcc cgcccccgcc atgagcatcg acttcagaca
     8041 gatcggcacc atcgccggca aggaattctg ggaccgcatc cgcaaccgct gggtgctcgc
     8101 ggtggccctc gtgttcaccg tcttcgccct ggtcatcgcc tacttcggcg ccgcccagca
     8161 gggcgccgtg ggcttccgct ccatcgacgt caccatcgcc tccctggtga gcctggtgat
     8221 ctacctgatc ccgctgatcg ccctcatcct cggcttcgac gccatcgtcg gcgaacggga
     8281 acggggctcc ctcgacctgc tcctgaccct gcccatcacc cgcctcgaac tgctcctcgg
     8341 caaatacgcc ggactcgccg ccgccctggc cttctccacc gtggccggct tcggcgcggt
     8401 gggcctgatc ctgtcgccgc agctcgatct caacgccctg ttccactacg gcggcttcat
     8461 gctctccgcc atcctgctcg gcttcgcctt cctcagcctg gcggtgatgc tctccgtctt
     8521 cgcggccgac cgcacccgcg cctccgggct cgccatcggc acctggttct tcttcgtgct
     8581 ggtgttcgac ctgctcctcc tgggcgcctt ggtggtcacc gccggcgact atggcggcag
     8641 cgtcttcccc tacctcctgc tgctcaaccc ggccgacgtc ttccgcgtcc tcaacgtctt
     8701 cagcctggac gaggtgcgca ccctctacgg cctcgccacc gtctttcccg aagccctcag
     8761 ccatccggcc gtgctgggga gcgtgatgtt cgcctggatc gtcgcgcccc tcgcccttgc
     8821 cctgtggaga ttccagcgat gaacattccc cgccccctgc gtcgcctcgg cctcgctgcc
     8881 ggtctcggcg cctgtgtcct ccttgccgcc tgcggcgatg ccgcccgcag cggcggcgcc
     8941 acacccgtcg agatcacccg cgacacctat tgcgccctgg acggcatgct cctcgccgac
     9001 taccccggcc ccaaggccca gattcattac gacggccagc ccgagccgga atttttctgc
     9061 gacaccaccg agatgttcgc gatctacctc aagcccgagc aggcccgcaa ggtgcgcgcc
     9121 gtgttcgtgc aggacctgga caagacgcgt tgggacgcgc cccagggcgc ctggatcgac
     9181 gcccgcacgg cctactacgt ggtcggcagc ggcaagcgcg gcgccatggg cccgacgatc
     9241 ccctccttcg gcagcgaggc cggcgcgcgg aagttcgccg aagcccaggg cggccaggtc
     9301 catcccttcg ccgccatcac ccccgacatg gcccgcctgg acggcggcgc cctgcacgac
     9361 cagaagatgt gagccctgcc cgagaccgac catggaaccc ttctacgcca acctgccccc
     9421 ggaactcgcc ctggacgtgg agcagcgcgc ccgcctcctc tacgcactgc gcggcgcccg
     9481 cgacgaagtc cttgcccgcc tcggcgccgg cgatgccgac caggccctcg cctgcctgcg
     9541 ccgcggcgag ctgccggaac acccggccta cgacgacatc ctctccgccc gcatcctcgc
     9601 cgacctgcac gcccaggtgc gaagcgatct ggccaccctg ctggccgaca gccaacgctg
     9661 aaaactccgc catgagcctc caccccgacc tcgccgcccg cctcgacgaa cgcttcggca
     9721 cgcgcttcga cgcccccgtc gaactcaagc tcgacgccct gctcctgcgc ctcgacaacg
     9781 gcgtgatcct cgaagcccgc atcgccgcgc ccgatgccta tgccatcgcc tgggtctggg
     9841 gcgacgccga gctgcgcatc gacaccgccc ccgtccatcc cgagcttgcc acctttcccc
     9901 atcatgtcca cggcgccgac ggccgcctgc gtgccgatcc gctcaccgtc tgcggcgccg
     9961 atcccttcga caacctcgtc cgcgtggtgg acgccctgct cgaagatccg ctgctgggcg
    10021 ccccgcccgg aactgcccca tgatccgctc gcccttcgcc gccctgggca ttgccgcctt
    10081 tttggccatc ctcccggcgt cggccgccga tccggcgccc cagcccgtcc cgcgcaacgc
    10141 ccgctgcgcc gtgtgcggca tgtacccggc ccagtatccc cagtggaagg cccagatcgt
    10201 cttcaacgac cagagcgccg cccagttcga ctcccccgcc gagctcttcc gcttcctgca
    10261 caacatgccc gcctttgacc gccgccacag tgccgccgac gtgggcgccg tgtgggtctc
    10321 ggattacgcc agcaaggcct gggtggacgc ccggaaggcc gtcttcgtcg ccggctcgcg
    10381 tgcccgcggc cccatgaacg acgccaacct gcccgccttc tccacccgcg aggccgctga
    10441 cgcccacatc cgcagccagg gtggccgtgc cctggcctac gaggagatca cccgcgaagt
    10501 cgtccaccac ctcgccggcg ggcacgccca ctgaacaccg cccttcccgt ccgtatcgcc
    10561 gccaggcccg gcggcaggtg catcacgccc actaacattc agcaacacaa cagtcccgat
    10621 cgggcacggt ccggggccga tttcgcgcag cccgcgtgct agcgtgaagg tggcgcccgc
    10681 gtcccggtgt tccagcgcgc gtgccggccc tccccgtccc ggaaaggatg tgtcccatgc
    10741 ccgccaccac cgtcagcgcc agcaacgtca tcgccgatcc caccaagacc ctgcagcgct
    10801 tcgacctgct cgatctggag caatacaccc aggaaaaatc ctacgctaca aacgccagca
    10861 aggacttcca cctcttctac gtgggccgcg acgacgtgca cgagatcctc aagcacatcc
    10921 tctcgcgggt cagcgtctcc ctgtacatga acatgttcgg cttcgatgac gaggagctga
    10981 acgacatcct catgcagctc gccaacgatc cgtccgtcgt gatgatgatc accctcgaca
    11041 agagccagac cggcggcgtc cacgaaaagc gcctgctcga ttccgacgct gcgaagaacc
    11101 cccacgcctt caacacctac ttcgtgatcg gcgaatcggc cacccaccag atcagccaca
    11161 ccaagggctt cgtggccgac ggccgggtcg gcggcgaagg ctccaccaac tggtccgcat
    11221 ccggcgaagg caccttcgtg gttcagggcc atgccggcgg gcccggctac aaggcccaga
    11281 acaacaccca gaccatcttc accgatccgg acaccctgag ccgcttccag gccgagctga
    11341 tcgccgagca cctcgccgcc cgtggcaagg cctgaccccc ggagacccgc tacacgatga
    11401 agccccagcg cacccttgtc ctgctcctga gcgtcgtcgc cctgagcgcc acggcagccg
    11461 tcgtcaacgg cggaggctac gtggccggcg acggcttcac cctgtccgag gccctcgccc
    11521 aggcccgcca ggaacatccg cccggcagct tccggccgtt ctggctgctg gccctcgacg
    11581 gcgcggtcgg cgccctcgcc cgccgcggcg ccgggccgga catggcaacc gccgtggccg
    11641 acatccagcg gcgcggcggc accgtctacg tgtgcgagca ggacctccgc gccgccggcc
    11701 tgaacagcgc cgaattgctc cccggcgtgc aggcggtacg gggctggagc gatgcggaga
    11761 gtggcatccg caacgaggac ggcgcgtcgg acgccggcgc agacgacgca gagcagcccc
    11821 tctccccgct gcgggcggtc aatcgcctgt gcggcagccg accgacgccc gacgactcac
    11881 gctgaaggtg aggccgggct gcgccgcgcc tcgaagccgt gcagacggta gccatcccgc
    11941 agggaccgca gcagcgtatt gaagagccag cgcggaatcc ccagggtgcc atgttcgacg
    12001 gcgcgggccg ggatggaggc gacgctgcgg gaggcataac cgagggattc cacgaaggcc
    12061 gccggatccg cccggtccgg cgcgaaatct ccgcccaggg cgcggatcgc gcgacgcagc
    12121 ccaccaccat agcgccgcgc gaaacgggca tccatcaggt cacagagcag cgtgtgtcct
    12181 ggcagcacgg cgtgcagggc ccgcgccgtg gcggcgaagg ccgcatccgt gaggtacatg
    12241 ctgacgcctt ccagcaccac gacggccgtt tcatggccgg cccagggggc cagaaccccg
    12301 gccagatcct gcgtggcgaa atcgatgccg acgcgctgca gcggcgacgg cgcggtgtcg
    12361 gccggcagcg cgtgttcctt gcgggccagc agcgcgggct ggtccagttc cacccagcgt
    12421 ccgcctggca gacggaaggc gcgggtgtca aagcccgctc cgaggagcac gatcagccca
    12481 tcgggatgcc gggcgaggtg tgcacgcaga aaatcatcca ccagccggtg ccgcgcggcg
    12541 ttcgagaggt tcgggccccg gaaacgggcg aaacgggcaa ataccgcgcg ggcctcatcg
    12601 tccatgaagc gggcggccag gtggtcaccg caaatcgggc ggggcgacgc cgcgtcggcg
    12661 gcacgcacac cacagcagta gaaggcagtc cgggcgatgg ggtccatggc aaggtctccc
    12721 ttaaaaatgc cggtgcggac agacagtcta cgcacacggg ccgacgctgg aggcatgacg
    12781 tccgcgcggg gcataaaaaa acccggccat cactgaccgg gtcttggcgc aggggggtct
    12841 gcgccgaggt gaacagtgac gccgcgccta ttcggccttg gacaccatga agtccacgat
    12901 ggtcttgatc tcgtcgtcgg cgaaggccgg gttgccgccc ttggccggca tggcgccctt
    12961 gttgccgttg aagccttcga tggcgtgctt gtagagggtg tccttgccct gggcaatgcg
    13021 cgggccccag tcggccttgt cgcccagctt gggggcgccc gccacaccgg tctggtggca
    13081 catggcgcag gtctttttga agagttcggc gcccttgccg ccatcggcgg tgtcggcggc
    13141 cggggccgca gcctggggcg ccggggcggc agcagcgggt gccggcggcg gcgcttcggt
    13201 cttgggggct tcggacttgc cgcaggcagc aagcagtgcc accagcagca cggggagaat
    13261 acggattgcg gtcatggaag ttctctcggt tggggaatgc ggagtcatgc cgtttggcag
    13321 ggtgcatcct agccaagcgc cggcgggcgc ctcattgacc ggcgtcaagt gccgcccgac
    13381 gtgccgtcca agccgtgccg gcgcgcggtt cagaagcgca gggttactcc cgcggtggcc
    13441 gtgcgcggca tcgagagcgt gcggttgtag ggctgattga tggtgtaggc gttctcggaa
    13501 tattcccggt cgaggagatt gaccaggctc acgaacaggt cggtacgccg gtcccaggcg
    13561 taacggacgc tggcattgac gaggggaatg gcgccctgct ggaggcgtgc cgtggcgcca
    13621 ttgctggtgg tatccaggta catgctgccg atgtagcgca gctcggtgta ggtgctgaaa
    13681 cgctcgacgg gctgccaggt ggtgcctaca gcggccacgt cccgcggggt ggcgaccagc
    13741 tggacgttga ggggatcggt gacgatgctg ccgtggcggg tcaggaaggt ctcggtgtgg
    13801 gtgtaggagg cgtctacgct gagggtggga cgcacctgcc agccggccac cagctcgatg
    13861 ccgtaggcgc ggccgtcctg gtcgttggtg tagtacttgg cggagccgcc gcagccgggc
    13921 agggtcggcc cgccgcagat cacctgcacc tgggccggcg cgctggcggc ggtggccgtg
    13981 taggtggcga tcatgttctt gatgttgtag tggaaccagg tggcgcccag gttgaagggg
    14041 ccgcgccgga agtccgcgcc cgcctcccag ccggtgaggg tttccggcgc caggtccggg
    14101 ttggcgatgg tggtgctggt gccggtgcca aaggtgcgcg tgaggttgtt gaagccgggc
    14161 gcgcggaagg cgcgatacac ggcgctgcgc agggacaggg cgtcggacag ctcccagcgc
    14221 agggccaggc tcgggttgaa ggcgttcttg gagccgtcct gcagcgcgcc accggacgcc
    14281 acgcccgcgg ccgtggtgcg caggttggca cgctcgtcga tgcgccaggt gtcgtagcgg
    14341 gccgacaggg tcacctccag cggatcgacc gggaagaact tgccctggcc gaagaggccg
    14401 gcgaaggtct ggcggccctt gccccaggtg gtgctgttga ggttaccctg aggtgccgtc
    14461 gggctggtcg gcgtggtgta gaaccactcg gcgtcctcgg cgcgcagggc gcggtaatcc
    14521 acgccgagct ggtagctgct gaagcgcccc gccagatcct ggctgaacac caccgaggtg
    14581 ccctgctcgc gatagcgctg ggagccgtac tgggtgtaga actcgacgac ctggtcattg
    14641 accttggccg gcgtcagggt ggcgctgttg ctgttgaggc aactcgtgcc gccctggtaa
    14701 taacaggtgc tgccgttgta cttctcgaac ttcacctgct gggcccaggc gctggctgtc
    14761 aggcgcgtct tgtcgtcgaa gcgccgggtc aggctccccg ccaggtccgg gctcttctgc
    14821 acgttgctgc cgtactggta gctgatgtcc tggtcctgga tgtggtaacc caggcgcagc
    14881 cagccgttca gatcggcgga cggacggaag taggcggtga gctggacgtt cctgttctcc
    14941 acggccaccg gctgcttgtg gggaaagcgc cacatgaatt ccgccggggt ggtctggtag
    15001 ccgttggact gatagtcgtc caccgtcaga ttgaagccca gggcctcgga tacggcgaca
    15061 ttcttgctca atgccacatt ccaggtgccg tagctgcccg ccgacaggct cacctcgccg
    15121 gcgtcgtcct ggacgcggcg ggtcacgatg ttcaccaccc cggccaccgc catgttgccc
    15181 cacaggctgg aattgccgcc gcgcacgatt tccacccgct ccacattgga caggggcacc
    15241 ttgaaccact gggtggtcat gtagaaagga tcgtggatcg gtacgccgtc cagcagcacc
    15301 agcaccttgg cattgcccag gccgcgcatt ctggtctggt ggccggtggg atcggactgg
    15361 ctcgccggca cgccggtgaa gttcatgccc ggcacgctgc gcaggagctg gtccagactg
    15421 cgggccggcg agcgctcgat gtcctcgcgg gcgatcaccg tagtgtgcag gggcatctcc
    15481 tccacgcggg tctcgctgcg gctggcggtg atcacgacgg gggccagctc tacgctgtca
    15541 gccgccgccc gggccgacag cgccgccggc agaagcgtca tcagcatgag ggccgcaggc
    15601 agcgggcgca ggcgtgtctt cacaatcaat tccttatttt cttattcggg gccaattccc
    15661 ctcccaattc ggagaattat ccggagattg atctgcgacc gccctgcgac caattgtcgc
    15721 agcgcgccgc gcggcgcgta cctagcaatt acaagacact gtttttttgc ataaaatcca
    15781 acaaaatggc gacgcctacc cgagcacgac attgcgcgca ccgcaaatag gccggtacca
    15841 tgatcggccc ggcgagaaca tccccacgca ataaagagac acccgccgga aattgacgga
    15901 tccaaattga ttcagcacaa gaaatgaaaa aaatcctgcc gatcctattt ccgttgctgg
    15961 ctgccctgac tcttccggcc gcggcccacg aaggccacga tcagggcccc gcggcgcggc
    16021 ccgcccgcat cgaactggct accgccgccg cctttggccc cgacggccag ctccatatcg
    16081 tcgccaagga tggcgcccac ctcgtctggc gccacagccc ggatctgggc gccacctggt
    16141 ccgccccgca gcacgtcaat atgcaagccg agtccatcgc cgccgacggc gacagccagc
    16201 cacgcatcgc cttcaaccgg gacggcgagc tcctcgtcac ctggacgaag cccctgccca
    16261 agccctacac cggcgagatc cgcctcgccc gcctgccccg cgacggcagc gccttcgacg
    16321 cgccgatcac ggtccatcgt gaccaggccg agatcaccca tcgcttccag aacctcatcg
    16381 tcgccccgga cggacgcgtc accgtggtgt ggatcgacaa gcgcgaccag gaagccgccc
    16441 gcctcaccca gaaaccctgg cgcggcgccg gcgtgtacag cgcaatctcc gacgatggcg
    16501 gacgcagctt ccagcccgag cgcaagctcg ccgaacacag ctgcgaatgc tgccgcatcg
    16561 ccctcgccct cgacccggac ggcacacccg tggccctgtg gcgccacgtc ttcgccccca
    16621 acgagcgcga ccacgccctc gcccgcctga aggccgacgg cacgcccgag gccgtgcagc
    16681 gcgccacctt cgaccgctgg cgggtggacg cctgcccgca ccaggggccg gccctcgcca
    16741 tcgctgccga cggcgtccgc cacgccgtgt ggttcaacca gcgcgatggc gaagggcggg
    16801 tcttttacgg ccgcccgacg tccgccggcg tgaacggcca gaggcctgtc ggcggccccg
    16861 ccgccgccca cgccgacatc gccgtcctcg gccgtcgcgt ggtgcttctg tggaaggaat
    16921 tcgacggcga acgcacacgc ctgcgcgccc aacgctccga cgacgacggc cgccacttcg
    16981 acccgcccca ggagctggac gccaccgccg gcgcctccga ccagccccgc ctcctcaccc
    17041 gcgacggccg cgcctacgcc ttctggcgca ccgccgacgc cttccgcgtg gtcccgctgc
    17101 catgatccgt gccaccctgg cgctccgcgc cgccctcgcc gccctcttca tcgccgcggc
    17161 gcccctgccc ggccacgccg cgacagagct ccagcccctc gaccgacacg ccgcccgcag
    17221 cctcacggac ccgcgcagcc acacgcgccc caccgccatc gccctgtggt cggcagaatg
    17281 cgagcattgc aagaagaatc tcgcggccct ggccgaactg gcccgccgcc acccacagct
    17341 gcgcatcgtc accgtcgagg ccgaacccgc gacggccgac agcgcccccc tgctggcccg
    17401 caccggcctg acccgtgacg agcgctacgc ctatggcgac gaatccccgg aggccctcgc
    17461 ccacgccctc gatccccgct ggcgcggcga gcttccgcgc acctacctgt tcgacggcca
    17521 gggcggctcg ctgacgcgct ccggcgtcct caccgcggcg gacatgcgca gcgcgctcaa
    17581 gctgaagtaa tccggcggcg cggaagggcg gccgcccgga ttccgcagaa accgcctcaa
    17641 tccttcgacg cggcctcccg tgcctccagt tcggcaaggc gatgcgagac ctcgaccatc
    17701 gcgtcgaaca ccggctcgaa gatccgcccc cgttcggtga gcgcgtacgc cacctgacgc
    17761 gtctggcccc gggtctcggt ccgcgccacc agccctgccc cttccaggtc gcgcagcttg
    17821 gcggtcagca ccttggccga gatgtttccg atgtcgcgcc gcagctcacc gaagcgtcgc
    17881 ggaccgaacg acaggaacca caggatccgg ggcacccacg cccccgacac cacgtgcagg
    17941 tagcgctcca gcgggcagcc ttcggggggc tgcggaacgg gctcggcatg ttctcgtttc
    18001 atcgcgtcct gactcgttga agatcaccgg ctggtgctga ttaccaagcg gtaactactt
    18061 gttttacata gagggtgctc ccatactgcg gccgccctga cgcccgtcga gccgcgctcg
    18121 acgggccggg gcagaacagc aaacccggcg cccgaccgtc gggacgaccc atgaacgatg
    18181 cggagaacat catgcacagc accctgcgcc tcaccctcct gaccctggcc ctcgccacgg
    18241 gcggcagcgc cgtcgcgtcc gacggcccga ccactgccga cttcaacgcg gctgcggacg
    18301 ataccagcaa ttggctgtac gcgacgcacg actacagcgg ccagcgctat tccaggctgc
    18361 gccagatcaa ccgcagcaac gccgcccggc tgcaggccgt gtgcatgtac cgttcggccg
    18421 acgccggccc ggcccagacc aacccggtcg tgtacgacgg cacgatgtat ctgtccatcg
    18481 cccgctccat cgtcgcgatc gacgccgcca cctgccgcga aaaatggaag tacacctggg
    18541 aaccgaaggg caacgaactg tcgcccacca accggggcgt ggcgatcaag gacggcaagg
    18601 tcatccgcgg caccgccgac ggctacctga tcgccgtgaa tgcccgcgac ggaagcctgg
    18661 catggagccg ccagatcgcc agctctacgg gtcagcaata cctcagcatg ccgccgctga
    18721 tcttcgagga catggtgatc tacggtcctg ccggtgccga tttcggccag aagggctggg
    18781 tcggcgcatt caacctgagc gacggcaccg aacgctggcg cctgcccttc atccccgatc
    18841 cgggccagcc gggtgccgag acctggtcga atcccgaggc gctcaagtac ggcggcggca
    18901 gcctgtggac gccggtcacg ctggatgcga aggccggcac cctctacgtg gccgttggca
    18961 atccggcacc cgacttctac ggtgacgcac ggccgggcaa caacctgttc acgaactccc
    19021 tgcttgcggt ggatgtgcgg tcgggcaaga tgctctggta ccaccaggcc gttccccacg
    19081 acatccgtga ctgggacctg acccacgccg ggcccctgtt ctccaccacc gtggagggcg
    19141 tgcgccgcaa cgtcatcgcc gccagcggca aggatggcct gctccgcctg atcgaccggg
    19201 acagccacga agtcctgtac acggtcgcct tcaccaccca ggaaaacgtg gacgttcccc
    19261 tcacggtcga aggcacgcgc tcctgtccgg gcctgctggg cggccaggaa tggaacggat
    19321 cggcctacag cccgcgcacc aacaccctgt tcgtgcccgc cgtcgactgg tgcaccacct
    19381 tcaagaaggc cgccaccacg cccgagttca aacgtgagga gcactattac ggcggcagca
    19441 tcgtcatgga cccgttcgac aaggcccgcg gctggctcac cgccctcgac gcctccacgg
    19501 gcaaggtccg ctggcgctac gcctcgggca cgccgatgct cgccgcagtc accgccaccg
    19561 ctggcgacgt gctcttcacc ggcgacttca accgggactt cctggccctc gacgccacca
    19621 gcggcaaggt gctctaccgc ttcaacacgg gcggtgaagt ggccggcggc atcgtgacct
    19681 acgccgtggg cggccggcag tatgtggccg ccgtgtcggg ctatgtgtcg gccttcttca
    19741 agggctccgg cccggccggc gtgacggtgt tcgcgctgcg ctgagggccc ccgccctgcg
    19801 cgggggcggc cgcctccgcg cgcaccgcgc ccagatgatg gagcagctcc cgcagtagca
    19861 gcgccgcggc gaagtaggcc ggatccgccg gcagcccttc gctcgcggcc gggcggccgt
    19921 gggcgatggc cgccaggcgg ctgaagagcc cctccaccgc atcccggtgc agtcccggcg
    19981 ccggccgctg gagatccgcc ggccagtcgc ccgacagacg caggacggcg cccgcaggca
    20041 gatgcaggcc gagggccatg atctgcggct cgatgaggtc gatggccgcc tccagttcgc
    20101 cggccgtcgg tggcgtgtgg tgcaggcagc gacgcaccag cgttgccggg ccgagcgcca
    20161 ggctgcggac ctcctccccg ccctgggcgg ccggccgcga cacgatcgcc tgctccgcct
    20221 ccaggcggat tgccagcgca ctcacggaac cgacctcgcc ggccggcgca ccatcgccca
    20281 gcagccgcgc caccagaccg gcggcgagcg cctccgagat cccatgcccg agcccgggaa
    20341 aacggtcgaa gctcactcgc gcccccagct gcgccagccg cgccaccgcg gcctccgcca
    20401 gaccgatggg catcacgggg tccgcctcgc cgtgcagcac gtgcacggtc gtggccgggg
    20461 ccacctgtgc gggcggttcg gcaaggcgcc cggcaatcgc caccacggta cgggccggcg
    20521 cgtctgcccg cgcgagggtc gcgagggcca tgatcgcccc ctgggagaag cccaccagca
    20581 ccgtggcctc ggccgccacg ccggtccgcc gctgccattc ggcgaccgcg tccagcagcg
    20641 tcggcagcgc ctcggcgacg cggcccggcc ggttggcgtc ggtcacgcct gccaccgaga
    20701 accattcccg gccgcccgcc gtcccggacg gctgcggccc ggcgaccgcc accaccgccg
    20761 ccgccggcag ggcccgggcg atccgctcgc ccagcggcac catcgccgcc ggctgactgc
    20821 cgacaccatg gaagagcaac accagccggt cggcgtgcgc cggcagcgcg acggggatgc
    20881 ttgaagacgg aagcatggcg gacctctcga gtgaaggcgg cattggagtt gccgcgggac
    20941 cagtaaaccg tgtccgggat ctgccggcaa gggcctcacc gggcgaacgc gcgcgcctgt
    21001 cgccgtttcg gaagacctgc cgacatgccc tgccacacgc tcatcgggca aagccccgcc
    21061 gcggcaggcc ggtgagcggc accccggccc ctcgtctgcg ggtcgcccgg ggcgccggcg
    21121 cacggcgagc ggatcgcggt ttaagatggc gcgtccgatc ctggagcaca ccatggccct
    21181 gccccgtccc gcccctgtcc tccgcctggc agccctactg ccgctggctg tcctgtgtgg
    21241 accgaccctg gccccggcac aggacgcggc cccctgttcg ccggcgcgtc tcgcagccat
    21301 cgaacgccag agccagcccc ttgaccgcag cggccacggc ccggaccgcg gctcggcgga
    21361 atgggcctcc gcggtggaat ggcgcctcgg cctgcgcggc cgggacgacc tgcccgcccg
    21421 cggcagccgc gcctggtgcg aacgcatcga agcggccctg cgccagcggg acgagcaggc
    21481 ccgcctgtac gcctcccgcc ccggcccggc cttttcctgc cgcggcgtgc agccgggcag
    21541 cattccggat ctggtctgcg ccgacacagc cctgggcacc ctcgaccgcg acctgcagcg
    21601 ggtgtatgcc caggccgccc gcaccgcagc gcacgaacgt ccgcccgtgc tcaaggccga
    21661 gcagcgcggc tgggtccggg gacgcgacga ttgctggaag tccgacgacc ggcgcgcctg
    21721 cgtggccgat gcctacgtca cccggatcgc cgaactgcag gcccgctacc ggctcgttgc
    21781 ccatcgcggc cccttccgtt tcagctgtaa cggccagccc gcggacgaag tgctcatcac
    21841 ccatttccag accgatccgc ccaccctcat cgccgagcgc ggagaccaga cgtccctgat
    21901 gttcctgcag cccgccgcca gcggcacccg ctacgtcggc cgcaatgaaa gcttctggga
    21961 acaccagggc gaggccacgg tggtgtgggg ccacggcgcc ccggagatgc gctgcatcct
    22021 cgaccgctga cgccgggcgt gttcccggcg ggaaccggct accgcctccc ctgctcaggg
    22081 gcggatcagg cgcacctcgc cactgcgccc gtccacctcc acctcatcgc cgtcctgcag
    22141 cccgtccagg atgcccggca gattgaccac cgccggcagg gcaaactccc gcgcgacgat
    22201 ggcgccgtgg gacatgtagc cgccggtctc caccaccagc ccggcggcct tgaggaacag
    22261 gggcgtccag cccggatcgg tggatggcgc gacgaggatc tcgccggcct gcaggcgact
    22321 gccctctgac gggtggcgca ggatgcgcgc ccgcccgcgg gcccgcccgg tgccggtggc
    22381 cacgccccgc cagccttcgc ccggccggtg cggcggcgcg ggccaggccg gggccgccag
    22441 ccattcaccg gccggggcga tgcacaggta ttcgggcggc gtcaccgcct gccacgcttc
    22501 gtaaagcgcc ttgcgtgccg ccacccgggc gcgcaggccg gccggtggaa caaggccatc
    22561 cacggcccgc tccgcctcgc tgggcagcac gaagaacaca tcgtcccgct ctgccagcgc
    22621 cccgtccgcc cgcagcaggt ccgccgcgag gcgccacgcc attcgacaca cctccagcag
    22681 ggcgatcagg ccgctgcggg ccgcctcccg ctggttgcat tcccggcggg ccgccttcac
    22741 cagggagcgg agcaggggcc gatgccagaa cgggacctcc cgccggacgc gctgccaggc
    22801 tgccgccgcc gcgtcctgct ggcgcgcgcg cagggccgcc tcgtccacct cggcgagggc
    22861 cggcagctgt tcgagcagac gctccggccg ctcccgccag gacgcgctgt gcatgtaggt
    22921 ctcgtagtgc ccgcggtgcc cgtggacctc catgaactcg gcgaaggccc gcgcatagtc
    22981 gccctgcgcc ggcacctgtg cggggaagcc ggccgcccgg gccagacgcg ccaggcggag
    23041 cagatcgtat ccctgctgcg ccgtgacgct gggcgtcccc ccggtcagca gggccgcccc
    23101 cagggcctcg ccctcgccgg ggaagaagtg ttccaggcgc tccaccagga aggacaggct
    23161 ggcaccgccg gatccttgca ggaagaaggc gccgatggac gtccgcccgg gccgcgacac
    23221 ggtgtacagc agccgccgca gggcgtcccg gtcccgcggc ggcggtccct gacggacctg
    23281 ccgcgcaagg gcatgcatgg ctgcgatctc cgcctcgccg cggcgccgca gggccggcgc
    23341 ccgcaccagg tagcgcaggg tcgtgagggc gcggcgcagg cgctcgcgcc cgccgggcgg
    23401 cgtcacgtcg atggccggct gatggccgcc catcaacgcg ttcaggcgct ccggcttcat
    23461 cccgatggcg tcccaggctt cccactggag cagcgacgcc tccaggtaca gccgcccctc
    23521 gaagagcccc gcccgctggg cccccgccag cacaggaaag ccggccagca cgtaaccctg
    23581 cacgagcaga tcgttcacac cccgccggga gaagttccag tccgccggct ggagcggctc
    23641 gggcatcacc tcgcaggtgt tgccgcgggt ccagatggcc ggctgggcct gcagcgggcc
    23701 atacgcgtga cgtggccggc gcgtcaccgg gcgggcctgg gtgagccaga agcgggcccc
    23761 gtcccacacc cattccagat cgtagaaggg cgccacgaaa tccatcgcca ccgccgcgtc
    23821 ccgcagcagc gccgccagcg cctcggcccg ctccgtgtca aagaggggcg ccgccgccgc
    23881 atccggcagc gccacgcgcc gcgtgccgcc tcctggggcc ggtacgctca tgcaggtctt
    23941 gggacccggc cggcactcga tcagccgcca ggcgtcctcc acgtcctcgg caaacacata
    24001 ctcgtcgccg gccgtctcgc cccccaccag ggtctcgccc aggccccggt tggcgtggat
    24061 cacgaggcgg tcctcgcgcc ccgtcagtgg atcgcaggta aagccgatgc ccgacgcctc
    24121 ggccgccagc aggggcatca cgatcactcc catgcgcgcg ggccccgcgt gccccatgcg
    24181 cgcgcgatag gcccgcgcgg tgtcgctgtc cagggacgcc cacaccgtct gcaccgccgc
    24241 gaccaccccg tcgagcccct gcacgttcag gcaggatgtg tagatcccgg cgaaggacgc
    24301 cgccgcggag tcctcgccga cggccgacga acgcaccgcc aggggctgct ccagccagcc
    24361 gcgcgccgcc agaccgtcgt gcagggccgt caccagcgca gcagggaagg cctcgccgcg
    24421 gccctcgctc caggctgcgg gcagcaccgc gaaatccggc accggcagac cgaagtggcg
    24481 cagttgggcc agttgccggc ccttcccgcc ggtcaccgtg gcgtcggtca cccgcacctc
    24541 ctcgggattc agcaaggcgc cgttcatgaa cgcacctcca gcccgcccag gaagaccgcc
    24601 agcatccgct cgaactccgc ctgcagcacc ggggtggcgc cggtcagcca gcgcaacagc
    24661 gacgccagat agaggaattc caggtaattc gccagcacct ccgccccgat gtcggtgcga
    24721 aatccgccgg cgccctgacc atccgcgatc agcccggtaa acacctgcgc cagcccgctg
    24781 cgctgcgaat cgtccccgtg gcagggctcc gcaagacgaa aacgcacata gggcaggagc
    24841 gtttcccgat gccgctcgct ccactgcgca ctcagatcga agaaacgcct caatcgggcc
    24901 gcccccggcg cctcggcgag caccaccggc tgcaggcgct ccagcccctc acgcagttcc
    24961 cggtggaaac gatgacgcag caaggcctcc ttgaccggga agtgcttgta cagcgtgccc
    25021 ttggccacgt ccgccaccgc cgcgatgcgc tccatcgtca cggcctcata accttccgcg
    25081 tcaaacagcg cccaggccgt gtcggcgaga tggtcgagct gcaggagccg cttgcgctca
    25141 cggcggcctg gatcggaacg ggggggcgtg gacatggatg acagacctca gtgatcgatg
    25201 cccggccgca ggcggagggc aaggcttgaa attgatacga aggtcaataa attaccatgt
    25261 ttatttttgt acgtcgttca ctattttaac gaccgcgcca cgccgtggaa ggtgccgggg
    25321 cccggcagcg cggcgcaatc cttcgaaata ccccgccgcg cgcggcgcgg ggttccgccg
    25381 cagcgcgccg tatgcgcccg tggcgcgggt ctgacggagc gctggccgac acgccctccc
    25441 gacgctgccg caaggcggct cagcttgcac gaccgtcccc gcgacacggt acgcctttag
    25501 cgagcaccct cagcccgcgc cccccagcgc ttgccgccac gccgccacca tccccggatg
    25561 gaaggccgcg tgcccgcagc catccaccca caccagccga ctgccgggaa tgcgggcgtg
    25621 cacgcgacgg gcgttgtccg gcgggcagac ccggtcgagg cggccgtgga tcagggtcac
    25681 cggcactccg ggcaggctcg cacaggcatc cagaaagccg gcctcggtga cgaaggcgcc
    25741 cgcttgcagg tagtgggcct gcacccggta cttgcgcacc aggcactccg ccgcagcggc
    25801 gtccaggtcg gcctgcggcg gcgcctcggg ttccgccagg gcgcgttcgt aggccgccca
    25861 ggccacggcc gcgcgcacgg cgcgggccgg ctcgggatcg tccagacgtc gggccagcgc
    25921 ggccagcaca tcgccaccgg ccgggcagcc ggccgcgtcg gccagggcga cccgctcggc
    25981 cggaacgtcg cccccggcgg cgaagaaagc cgccacgttg ccggcacccg gcaggaagac
    26041 gctgcgcgca acgacaccgc tgaccgccgc gggatgggcc gcggcgtaga gcagcgcgag
    26101 ggtcgcgccc caggatccgc ccgtcacccg ccagcgggcg atgcccaggt ggcggcgcag
    26161 gcgctccagg tcggcgataa ggtgggcggt cgtgttggca tggcaggcgc ctgccggcgc
    26221 ggagcggccg gcgccgcgct ggtcgggcat caccagccag tggcgggcgg gatcgtagag
    26281 ctccgccagg cggggcgaac agccgctgcc ggggccgccg tggagcacca gcaccggctc
    26341 gccgccgggc gcaccggcag tcgccacgaa gagacgatgg ccgtcggaga cgtccagata
    26401 aaacgccgca ggcacggcac aggcgggtga cggggacatg gagagggatc gacggaaccg
    26461 cggcgcaccg gccgcacccc gccagtgtcg ccaccctcgc cggacaccgc caacccaccg
    26521 ccgcaaattc atgattttcg tgaggcgggc cgcgcgggcc gtccccacac tacgctccac
    26581 ggatcggcaa gcgccggccg tttcatccac ccctcaagag gagacacgtc atgacctgga
    26641 ccaccccgca cgcctgcgac ctgcgtttcg gcttcgaaat cacgatgtac atcgccgcca
    26701 agtaacgccc tggcgccgcg atcggccgct tcagcccccc tgggcggccg tcccgccttc
    26761 accaacccac gccaatctcg acccatgcgc gttcatgtgc tcggcgccgc tgccggcggc
    26821 ggatttcccc agtggaactg caactgccgc aattgccgcg gcctgcgcga cggcagcctg
    26881 tcggcccgcc gccgcaccca gtcgtccatc gccgtgagcg ccgacggcgc ccgctggacc
    26941 ctggtcaatg cctcgccgga catcctccag cagttccagg cctttgccgg cacctggccc
    27001 accgacggcc tgcgcggctg cggcgtggaa tccatcgtgc tggtggacgc ccagatcgac
    27061 cacaccaccg gcctctacat gctgcgcgag aagggcagcc cgtggccggt gtggaccacc
    27121 gacccggtct atgaagacct cacccgcggc aaccccatcc tgcacctgct cgaccactac
    27181 tgcggcgtcg accgccgcgc catccccctc gacggccgcg tgttccggcc ccagggcgcc
    27241 gaggcactgg agtggcgcgc gatgcccctg ctctccgccg cgccgcccta ctcgccccac
    27301 cgcggccagc ctgtgcccgg cgacaacatc ggcctcacca tccgcgaccc cgccagcggc
    27361 cgccgggtgt tctacgcacc gggcctggcc gaggtggacg acgccgtctt cgacgccatg
    27421 gccggcgccg actgcgtgct ggtggacggc accttctgga ccgacgacga gatgatccgc
    27481 ctgggcctgt cgaccaagcg cgcccgcgac atcggccacc tggcccagag cggccccggc
    27541 ggcatgatcg aacagctcgc ccgcctgccg cgccacgtcc ggcgcgtcct catccatatc
    27601 aacaacacca acccgatcct cgacgaagac tcgccggagc gggccaccct caccgccgcc
    27661 ggcatcgaag tggcctgcga cggaatggag atcgccctgt gaacaccccc gtgaagcccg
    27721 ccccctggac gcctgccgaa ttcgaagcgc agctgcgcga caagggcgcg gcctaccaca
    27781 tccaccaccc cttcaacgtc ctgctcaaca cgggtggagc gcgcccggag cagatccgtg
    27841 gctgggtggc caaccgcttc tactaccaga tcatcattcc ccagaaggac gccgcgatcc
    27901 tcgccaactg cccggaccgg ggggtccgcc gcgaatggat ccagcgcatc cacgaccatg
    27961 acggccacgg cgacgacgcc ggcggtatcg aggcctggct gcgcctgggc caggccgtcg
    28021 gcctcgcccg ggacgagatc accgacctgc gccacgtcct ccccgccgtg cgcttcgcct
    28081 gcgacgccta cctcaacttc tgccgccagc gaccctggca ggaagccgtg tgcgcctccc
    28141 tcaccgagct cttcgccccc accatccaca agcagcgcct ggccaactgg cccgagcatt
    28201 acccgtggat cgaatccgac gggctgcagt acttccgcaa ccgggtcacc caggcccgcc
    28261 gcgacgtgga acagggcctc gccatcaccc tcgcctactt cgacacccgc gccgcccagc
    28321 aacgcgccct ggacatcctc aagttcaagc tcgacatcct gtggaccatg aacgacgcca
    28381 tggcccagca ctacggcctc gccggcggcc gcccggcctg aacccacgga aagcccccgc
    28441 catgcattcc acgcccgccc catccagcca tccgcgcatc ggccgcctgt tccgcttcca
    28501 gtgggaaccc gcccagcagg cccacgtcct gctctacccg gaaggcatgg tcaagctcaa
    28561 ccgcagcgcc ggcgagatcc tcgcccgctg cgatggcacc cgcagcgtcg ccgagatcgt
    28621 cgccgagctg gaggccgctt tccaggccag cagcctcacc gccgacgtgg aggccttcct
    28681 gcgcctcgcc gaagagcgca gctggatcga ctggaccccg gcctgaaagc cccgcgatgg
    28741 aagcgctcct catcccgccg cgccccggcc ccctgtggct gctcgccgag gtcacctacc
    28801 gctgcccgct gcactgcccc ttctgctaca acccggtgga tttcgcccag gccgggccgg
    28861 agctctccac caacgactgg ctgcacgtga tccgccaggc ccgcgccctc ggcgccgccc
    28921 agttcggcct ctccggcggc gagcccctgc tgcgggacga catcgagatc ctcgtcgccg
    28981 aagcccgccg cctgggcttc tacaccaacc tcatcacctc cggcgtgggc ctcaccgaag
    29041 cgcgcctcgc ggccctcaag gacgccggtc tggaccacat ccagctttcc ttccaggacg
    29101 ccacccgcga ggccaacgac ctcatcacga acacccgcac ctttgatctg aagcgccgcg
    29161 tcgcggccct caccaaggcc gccggctacc ccacggtgat caactgcgtg ctccaccggg
    29221 gcaacatcga gcacctggac cgcatcatcg agatggccgc ggagatcggc gccgaatacc
    29281 tggagctggc caacacccag taccacggct gggcctacct caaccgcgcc gccctcatgc
    29341 cgaccgccgc ccagctcgcc gaagccgagc gggtcaccga acgctggcgc gtccgcctcg
    29401 gcaaggccat gcgcatcctc ttcgtcacgc cggactacgc ctccacagcc cccaagaagt
    29461 gcatgaacgg ctggggcgaa gtcttccttg ccatcacgcc ggacggcctc gccctgccct
    29521 gccacaccgc gcgcatgctg cccggactgg atttccccga cgtccgcgcc cacgacctgg
    29581 cgtggatctg gcacgactca cccctcttca accgctaccg gggcaccgcc tggctgcccg
    29641 acccgtgccg gagctgcccc gacaaggaag ccgaccacgg cggctgccgc tgccaggcct
    29701 tcatgctcac cggcaacccg gacgccaccg acccggtgtg tcccaagtcg ccggaccacg
    29761 ccctcatcca gaccgccctg caggccgccg agcagccgac tgcccagcca ctggtgttcc
    29821 gcgacccgaa gcagtccgcg gccctcgccc gccccttctg acccgcccgg ccccggccgc
    29881 gcaagcgcct cctcaaaaca ccgcggccgc cggaagctca tcgcttcggg cggccgcggc
    29941 ggacgggcga tgcactcaga agcggaagcg cgccacgttt gacttgaggg cgtccgacac
    30001 cgacgacaga cgatgggccg tctcggccac cgaccccacg gccgccgcat tctcctcgga
    30061 catctgggcg atggattcca cgttgcgggc cagctcggtg gacgccgccc gttgttcctg
    30121 caaggcatcg gagatgctct cgatcacgcc gctcatggtg gacgcggatt cgcggatttc
    30181 gctcatggat tcgctcgccg tccgcgccga gcccaccacc gccgccacca gatcgcggct
    30241 cacctgcatg ctgttcaccg cgcccaccgc gccctgctgg atcgcgccca ccacgttgct
    30301 gatctcctgc accgaactgg tggtgcgttc cgccagcttg cgcacctcgt cggccaccac
    30361 cgcaaatccg cggccctgct cgccggcccg ggccgcctcg atggccgcat tgagcgccag
    30421 cagattggtc tgatccgcca cttcacggat caccgtcgtg atcttgtcga tctgctgcac
    30481 ctgctgggac agggtctgga tctgctgcga ggtggtctcc acctggctcg ccacctgccc
    30541 caccttgtcg gccgcggtat ccacgccccg ggcactgtcc accgccttgc tgcccgcatc
    30601 ggccgcccgc actcgggcat cgccggcgct ggaacccacg tggtcgatgc tcaccgtcag
    30661 ttcttccacc gccgccgccg ccgccgccgt gttgcgcgac tgctgttcgg tgctcaccga
    30721 cacctgctgg gccgccgacg acagctgggc cgcgtagccc accacgttct ccgcattcgt
    30781 ggcgatgccc gaaatcgtct gggccagatc cactcgcatc gcgtccaggg aggccagcag
    30841 ctggccgatc tcgtccgaac cgctgttcgg tgacgccttg ccgcctgtca ggtcgccggc
    30901 cgccacacgg gctgccaggg cgttggcctc ctcgagccgg ccggcgatgg cgcggatcgt
    30961 catcaggctg atcaccccca tcgcagcaca ggccaccacc aggatgccca ccgcaaacca
    31021 ggtggccgaa tcccgtgcag ccgccccttc cgccgccatc ttgtggccca gatcctcgtt
    31081 gaacttcatg tgggcttcca gctgctcgat gaaatgcacc gcgtccggcg caaacttggc
    31141 catcagcgcg cgggcctcat cgttgcggtt ggcccgcgag gccaccagca cctcgtctat
    31201 gttcttgttg taccgatcga aggaggcccg ttccgcctcc agcaggcggc ggtcctcgtc
    31261 gtcggcaatc agaggctcgt agtccttgaa gaacttccgg acgtgctcgc gccccgccat
    31321 gatggtcgcc tcgacctcct gcatggcctt ttcatcggta ttcatcacat ggcgatagac
    31381 ccgcaccctc agccttccga actccacggc gactttattg agcacctcga tgctcggcac
    31441 cacgttgatg ttgccgaagt tggctgcgtc atacaccctg ttcatctgca cataattcac
    31501 ggcagtcatt gcgagcagac tgatgagggc ggctcccacg agcaggagca ggcgtttaac
    31561 gatggtcatg gcagtacgtc caaaatgtgg atcggcagtc gctgaagcag gcggtgacat
    31621 tttcctgtcg gtggcgaccc atacggggag gccgctctgg cgtcaccaac ggccgccgtc
    31681 cgctataact ttaggcagag ccgtcggcga cacatctttt tgccggacgc gccccgccgg
    31741 gcgctccatc cggcctgccc gccacagcca cacggctgca acatggtgcg gttccaatgg
    31801 ccggatgaaa gcccgcgccc gccttgcctc cgacctgccc gacggggtcg tcatcgcccc
    31861 cctcgacgac ggccccggcc ccctgcgtga ggccgccgcc gccatcctgc tggccagctt
    31921 cgacgacccg gaacgctacg gcccggcgcg catccagtcc cagctcgaca gcgccccgct
    31981 gccctattac cggcgcttct tcatcgcccg gcgcgacgat gaagtgctcg gcgtgggcgg
    32041 catcaaggcc gccgactggg ccagcgacac ccacctgctc tacctctcgg cggtcgccga
    32101 acacgcccgt ggccagggca tcgcgcgggc cctggtgatg acccgcgtcg cctgggtgat
    32161 ggagaccttc gagcacggcc gcgtgctcgt ctccacccca cgcccgcggc gcttcgcccg
    32221 cttcggcttt cgcgccgccg gcaggctgga aagcgccggc cggcgcctga tgctgctgga
    32281 gttctgaaca cgccgcagca cgcatgcgcc cgaagcgcgg agaagacacg gggcccgcgg
    32341 cgcaagggct tgtataatcg cggacttttc tgcctttccc gcaccgcacc atgtccgccg
    32401 cccacgaaat tgcccgccgc cgcaccttcg cgatcatttc ccaccccgac gcgggcaaga
    32461 ccaccctcac cgaaaagctc ctgtggttcg gcggcgccat ccaggtggcc ggcgagatcc
    32521 gcgcccgcaa ggccgcgcgc cacgccacct ccgactggat ggagctggaa aagcagcgcg
    32581 gcatctccgt cacctcgtcg gtgatgcagt ttccctaccg cgactgcgtc atcaacctgc
    32641 tcgacacccc gggccacgag gacttctcgg aagacaccta ccgcaccctc accgcggtgg
    32701 actcggcggt catggtcatc gactcggtga acggcgtcga agcccagacc atcaagctcc
    32761 tcaacgtctg ccgcctgcgc gacaccccca tcgtcacctt catcaacaag ctcgaccggg
    32821 aaggccgcga gcccatcgag ctgctggacg agatcgaatc cgtgctgggc atccagtgcg
    32881 cccccatgac ctggcccatc ggcatgggca aacgcttccg cggcgtctat cacctctaca
    32941 acgacaccgt ccagttcttc gacccccagg ccgaaaaggg cacctccgag atcatcgaag
    33001 gcctcgacaa cccgcgcctc gacgccctca tcggcgacca ggccgacgag ctgcgcctcg
    33061 acatcgagct cgtccgcggc gcctcccaca gcttcgaccg ggacgcctac ctgggcggca
    33121 agcagtgccc cgtgttcttc ggctcggcgg tcaacaactt cggcgtgcag agcctcctgg
    33181 atgccgtggt ggatctctcc cccgcgcccc tcgcccgccc cgccaagacc cgccaggtgc
    33241 agcccaacga agacaaattc tccggcttcg tcttcaagat ccaggccaac atggacccca
    33301 agcaccgcga ccgcatcgcc ttcatccgcg tgtgcgccgg gcgcttcgag cgcggcggca
    33361 agctcaagca ggtgtccacc ggcaagtccc tcgccatcaa caacgccatc accttcatgg
    33421 cccaggaccg caacaccacc gacgaagcct gggccggcga catcatcggc atccccaacc
    33481 acg
//
LOCUS       DAOQPA010000027        33324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_572_length_33324_cov_18.989991, whole genome shotgun sequence.
ACCESSION   DAOQPA010000027 DAOQPA010000000
VERSION     DAOQPA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_33324_cov_18.989991"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKX01_05835"
     CDS             <1..492
                     /locus_tag="PKX01_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ75679.1"
                     /translation="AAQFEKWSLPLAVIMAVPFAMFGALSAVLMRSMPNDIYFQIGLV
                     VLVGLAAKNAILIVEFAAQKLDEGMGVVDAAVEAARLRFRPIVMTSLAFVLGVLPLAL
                     STGAGAAARRSMGTGVVGGMLAATFIATIFVPLFFKWLSRKGKGSGRSTPPATAATEE
                     VKS"
     gene            489..1928
                     /locus_tag="PKX01_05840"
     CDS             489..1928
                     /locus_tag="PKX01_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ75680.1"
                     /translation="MSPARPTPALLAVLLAAALGGCAVGPDYVRPESATNLPAQFAPG
                     SAKLAYSPSVASTWWTLFGDRQLDALVGKALANSPDALTAAARVEEADAVIRQVDAAL
                     LPQVDATAAGTHSRISQTTATPLPSSVAAVRNNARLALTTSFELDVWGKLRRASEAAR
                     AQALGSRYGRDTVALSIAGLVTQGYLNLRALDAQRLVTAESVASAERSLKLTQVRFDG
                     GIASQLDVQQADSALATLQAALLQLDQAREQAENLLGYLVGEPGLKIAAGDLRDLPIP
                     PVPPAGLPSSLLEARPDVRQAETDLIAANAKIGVAKAALFPGITLTGNFGRESKDLSQ
                     LFTSPSSVWALGAGITQPIFEGGRLRAQVDQVTAQQKQVAEAYRKAVYTAFKETNDAL
                     ASLRVGADAEDTLARALNAAKRNLMLAQARYDGGYSPYLEVLTAQRSANDAALAWIQN
                     RQARLAASVDLFRALGGGWQDSQKLAANP"
     assembly_gap    1998..2097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2327..3277)
                     /locus_tag="PKX01_05845"
     CDS             complement(2327..3277)
                     /locus_tag="PKX01_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ75681.1"
                     /translation="MTRSARYIVLHETEYTPCGVPVSLAQHLLHLMPRALPWQTVEKA
                     ELTVDPDLARIQQGEDVFGNPVAWMALDTPHDGLLVRAESRVTVLGRTLPKAEESPSW
                     ESVREIFHYHSGARDADSLDAQRYLYSSPRAPRDARLAAFAALAFKPETPLLAGVQAL
                     MEIIYKTFTFDAKATEVSTPVFEVLERRRGVCQDFAHLMIAALRSVGLPARYMSGYLL
                     TDPPPGKPRLVGADASHAWLAVWCPENGWVEYDPTNGIQPDLRHVTLGWGRDFQDVSP
                     LRGVILGGQAQAPEVRVTVMPVDEWEARQARAVRSKAPAP"
     gene            complement(3280..5808)
                     /locus_tag="PKX01_05850"
     CDS             complement(3280..5808)
                     /locus_tag="PKX01_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HMZ75682.1"
                     /translation="MPSSILSHYPYRTDAYDELFGPDGVRPHWQDFLDHVDESSADTL
                     KARAAQLARGIQQNGVTYNVYADARGTDRPWSLDLLPLILPADEWREISTAVAQRATL
                     LNALLADLYGPQTTLAEGLLPPELVYGHKGFLWPCQGIQPPGGTYLHLYAVDLARSPD
                     GRWWVIADRTQSPSGAGYALENRLLVSRAFPDLFRDLQVQTLAAFFRSLQRSLAAHAP
                     CDGNQVPLTVLLTPGPFNETYFEHVYLARYLGFPLVEGQDLTVREDTVYLKTVSGLKR
                     VHAILRRLDDDFCDPVELRADSALGVPGLLGAVRAGRVLVANALGSGLLESAALPGFL
                     PGICRHLLKEELAMPAVATWWCGEDPALDHVLGHLDSLVIKPTFPGGLSEVVFGDQLD
                     EDQREALRVRIAANPSAWVAQELVQLSGAPAWAGGHRRRLINRSMGLRVYAAASPEGY
                     TVLPGGLTRVAGMENARLLTMQKGGSSKDTWVLSDGPVSTFSMLPKNLRVADLVKVTG
                     TLSSRLAENLFWFGRYSERCDAVARLARLTLARLSEGRRTERSAALAMLLELAAGAEL
                     VERDVVRTSLGKAELALFRAVLDRRAGNGLAANLQRLTWCATQAREHLSLDHWHALKR
                     LSAEIEDCRGRVSDTSALLEFLDEALTTFVSLSGFAMDNMTRDMGWCLHILGRRIERL
                     DSLGSTLAHFLRCHADTAQPLQREMAVEALLELCDSIITYRTRYRAQPELLPAIHLLV
                     MDDANPHALAFQVRMIINYLGKYEQDGDGIGIAGLEEALYVIEHFSWESLERVRPDAE
                     RAAASLALAHALDALVHAGRELSDRLAMRFFSHVGRGSQATFAA"
     gene            complement(5951..9340)
                     /locus_tag="PKX01_05855"
     CDS             complement(5951..9340)
                     /locus_tag="PKX01_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ75683.1"
                     /translation="MTIHVALNHLTSYKYDRPVTLGPQVVRLRPAPHSRTRILAYSLK
                     VLPKPHFINWQQDPQSNYLARLVFPEKVTEFTVEVDLVAEMSVINPFDFFLEPEAENY
                     PFEYAGNLSHELAPFRLKDAPTPLFQAYLDSVPRDSRRVVDFLVDINAKLQQHIGYVI
                     RMEPGVQAPEETLEKRTGSCRDSAWLLVQLLRHLGFAARFVSGYLIQLKADVKALDGP
                     SGTEVDFTDLHAWTEVYLPGAGWVGLDPTSGLFAGEGHIPLAASPDPTSAAPITGLVD
                     ECECEFAHHMSVTRVLEAPRVTKPYTEEQWAEIEALGHVIDEDLKAHDMRLTMGGEPT
                     FVSIDDPDGAEWNTEAMGPTKRVRAEDLYHRLRDKYGPTGLAHFGQGKWYPGEQLPRW
                     SLNCYWRKDGEPIWTHPDLIADSATPGDADADLAGAFLRDVAERLGLDARRVFAGYED
                     AFYYLWRERRLPGNVDPFDARLDDPLERDRLRRVFDYGLANAVGYVLPISINTYTGAW
                     QTGPWFLRDERCYLIPGDSPIGYRLPLDSQPWVTATSYPYVNPPDAHQSFPPLPEYQA
                     IRRQLPGGQTAVLNALNAFKDDETYGLGSGAAARAAALKAARAAEKLSPTDRTPQSFE
                     SADWIVRTAMCVEPRNGTLHIFMPPLSRLENYLELVAAVESSAEAFGTPVVLEGYEPP
                     TDPRLTHFRITPDPGVIEVNIHPAGSWEELVDRTTHLYDAAHFSRLTTEKFMVDGRHT
                     GTGGGNHFVLGGATPSDSPFLRRPDLLRSLISYWHNHPSLSYLFSGLFIGPTSQAPRI
                     DEARNDSVYEIETAFRQFPKVGDSVPPWLIDRMLRNLLIDVTGNTHRAEFCIDKLYSP
                     DGPAGRLGLVEMRAFEMPPHARMSLAQQLLLRGLLARFWDTPYAPERLVRWGTELHDR
                     FLLPHFVWQDFSDVIEDMQGHGLDFKPEWFAPHQEFRFPKIGDFAARGIEVELRGALE
                     PWHVMGEEGAIGGTVRFVDSSVERMQVKVSGMSDDRYLLTANGRSIPLQPTGTVGEFV
                     AGVRYRAWQPPSCLHPTIPSHAPLVFDLVDTWTGRSLGGCQYHVSHPGGRSFETFPIN
                     AFEAESRRLARFFRMGHTPGAPKFETSAVEKEFPFTLDLRR"
     gene            complement(9599..11116)
                     /locus_tag="PKX01_05860"
     CDS             complement(9599..11116)
                     /locus_tag="PKX01_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMZ75684.1"
                     /translation="MTEPLLIAKSGPKDIVLLPALANRHGLITGATGTGKTVTLQKMA
                     ESFAAIGVPVFMADVKGDLSGIGAASNVSPKLKERLTALGIDDFTPRANTAVFWDVFG
                     TSGHPVRATVSDMGPLLLARLLNLNDTQAGVLQLVFKIADDQGLLIIDLKDLRATLQY
                     VGDNAKDFTTQYGNISAASIGAIQRGLMTIEAQGGDKFFGEPMLDIADLIQTDGDGRG
                     VVNILAADALYNSPKLYSTFLLWLLAELFEHLPEVGDLDKPKLVFFFDEAHLLFNEAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQSPLDIPENVLGQLGNRVQHALRAFSPKDQKAV
                     KTAAETLRANPEFDAATVIMELGVGEALVSFLDEKGRPSIVERGFIVPPASRLGPLSG
                     EERKAAIENSVIFGHYEQIVDRESAFEKLRGAPAAQAAPAGAPGGASAPAPAQDGGGL
                     LGGLGDILFGTTGPRGGRHPGLAETAARSVVRTIGSQVGREIVRGVLGSILGGRR"
     gene            11323..12051
                     /locus_tag="PKX01_05865"
     CDS             11323..12051
                     /locus_tag="PKX01_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMZ75685.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGRVFSRLAKEITVAARMGGGDPG
                     FNPRLRLAVDRAKAVNMPADNIDRAVKKGTGELEGVTYEEARYEGYGIGGAAVMVDCL
                     TDNKTRTVADVRHAFNKFGGNMGTDGCVAFQFKHCGQLLFAPGTSEDALMEAALEAGA
                     DDVVSNEDGSIEVLTEPYQFGEVKDALEKAGFTPEFGEVTMKPLNEAELTGDDAVRMQ
                     KLLDALESLDDVQAVYTSAVMDDA"
     gene            complement(12139..13083)
                     /locus_tag="PKX01_05870"
     CDS             complement(12139..13083)
                     /locus_tag="PKX01_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ75686.1"
                     /translation="MTHAPAFAENSSSRESVHARPSRVVTGAGGAGHQPCILFADVAG
                     GVRLAAKLGDAEAGYAVERCLNRMSRCAEAYATSGIEVRPDVLVARFADADAALYAAR
                     EMRERVRALPPMSGIKLVLRAGLVLESEAPDDLKGAETLASRLAVGHNPDTVSVSEAF
                     SEALSPAMRKLLSSMVQANDQLPFQVLELGEATPPSPVELRHASVRPKAQMKVSFQGR
                     SWTVDASSPTLLFGRETGNDIVVVDPRVSRQHARIELRNGLFYLTDSSTNGTYLLEEG
                     AAEHCIKRDDFILGEKGYIGCGFSVADNMSGAVAFALG"
     gene            complement(13080..14024)
                     /gene="ttcA"
                     /locus_tag="PKX01_05875"
     CDS             complement(13080..14024)
                     /gene="ttcA"
                     /locus_tag="PKX01_05875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HMZ75687.1"
                     /translation="MDLAVGAALPLPTDTPTPESRTSNTFRNLKRRLERKVGEAIADY
                     GMIRDGDVVMVCVSGGKDSFSLLSILLALQKRAPVNFRIVAMNLDQKQPGFPDHVLPA
                     YFESIGVEYRIVTENTYKIVKDKIPEGKTTCSLCSRLRRGIIYRTAKELGATKIALGH
                     HRDDMIETLFLNMFFGGKMKAMPPKLVSDDGQNVVIRPLAYCPEKDIERFARGMGYPI
                     IPCNLCGSQENAQRKQIKAMLRDWDARFPGRIVSIATALQNVVPSHLADGKLFDFLNV
                     SCDTKVDQGDLVFDAPELPSVPPAEAAVSLGFLREKSP"
     gene            complement(14091..14840)
                     /locus_tag="PKX01_05880"
     CDS             complement(14091..14840)
                     /locus_tag="PKX01_05880"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HMZ75688.1"
                     /translation="MSSASPAPVMLVTGGARRVGAAIVRRLHGEGARVAVHCRHSRDE
                     AEALAAELNARRPGSVAVLQADLLDTAVLPALVSAVVARFGRLDGLVNNASSFFPTPM
                     GRMDEAAWTDLVGSNLKAPLFLSQAAAPHLLATRGSIVNIVDVHAERPLHGYALYCAA
                     KAGLLGLTRALAVELGPAVRVNGVAPGAIEWPDDGQFPPAAQDAVVRHTLLGRVGEPD
                     DVARTVVFLLCNAPYITGQIIAVDGGRSAHL"
     gene            14974..16134
                     /locus_tag="PKX01_05885"
     CDS             14974..16134
                     /locus_tag="PKX01_05885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ75689.1"
                     /translation="MNLPPPSPDALAQSERLVARLRDLIAREGGWISFATYMGEALYA
                     PGLGYYSGGSRKFGPGGDFITAPELTPLFGQAVAGQLVEIMAQSAPQLIEAGAGTGLL
                     AADLLLELEHRGALPERYAILEVSGELRSRQFDTLAEKVPHLASRVCWLDTLPEHFSG
                     ALIANEVLDVMPVHLVAWRPEGLFERGVAMDDAGRFVWADVPASGRVAEAAAALERPL
                     PSTGEYVSELNLAGRAWIGEWAARLDKGALLLVDYGYPRAEYYLDSRSTGTLLCYYRH
                     RAHPDPFLWPGLNDITAFVDFTAMAEAGFDAGLDVLGYTSQATLLLNCGVLECLARRA
                     PEDSPDYIRASRAVQRLTGPHEMGELFKVIALGRGIDGPLLGFRRGDRLHTL"
     gene            complement(16187..16405)
                     /locus_tag="PKX01_05890"
     CDS             complement(16187..16405)
                     /locus_tag="PKX01_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75690.1"
                     /translation="MVIYMDMESGAQARMDPVDEQAPEYGDEILNANLLPPELALGLQ
                     EVAVTHAERHFVPTDVDAFLSAIYRFQE"
     gene            complement(16601..17404)
                     /locus_tag="PKX01_05895"
     CDS             complement(16601..17404)
                     /locus_tag="PKX01_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75691.1"
                     /translation="MKSARFGDLEVLCRLPSGTPRPTPLLFVHGAFTAAWCWEEHFLP
                     HFASLGYAAYAVSLSGHGSSRNRAFLDSYSLADYVADVADVADRLPAPPVLIGHSMGG
                     MVVQKCLERQRVPAAVLMAAVPPQGLLGSAFGLAFSRPNMMGDLNRIMGGGQPDLDTL
                     RDALFHEPVELVRLRQYYHLCQPESHRAIWDMSLFDLPALGHVALPPMLVLGAEHDML
                     IPPDQVRMTARRYGTEAYIVPRLGHGMMLEAGWRIAADPIAAWLAAHGL"
     gene            complement(17675..18085)
                     /locus_tag="PKX01_05900"
     CDS             complement(17675..18085)
                     /locus_tag="PKX01_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75692.1"
                     /translation="MEDTMKPWVQGLLLGLCASGALAADPPQCRQQIVEKSTLELCLV
                     PGAALQHDLYVLKADRQVIFALVDDFAEKVELEHEIPEGLVVEFPLSLQGEKIVKISG
                     GCRPESKEGLEVARVCNFHWGKHHVVKDVRFEFK"
     gene            18170..18466
                     /locus_tag="PKX01_05905"
     CDS             18170..18466
                     /locus_tag="PKX01_05905"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HMZ75693.1"
                     /translation="MLGARRRIPLSKVLPRSGRLRFNTAFVLPVPRPMLKWLLAIVVI
                     AVVSGLGRGISPGRWRIGHLPGDLHFHLWGRHIHIPFTSTLLLSLAAMLVIRLL"
     gene            complement(18463..20529)
                     /locus_tag="PKX01_05910"
     CDS             complement(18463..20529)
                     /locus_tag="PKX01_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ75694.1"
                     /translation="MRLLRVPSLPGLRPCRLSGPRAGVRPRRASGPETRIGAVLLAAV
                     LALPALPARAHQPLPAFADVKAAARPSVAVLLARDGTPLAERRLDPQVRRLEWVGLPA
                     LSPVLKEALLAAEDKRFFEHSGVDWQAFAGSLWHNLWNSRKRGASTLSMQLAGLLDPE
                     LALGRDGQPRRSLGQKWDQAFAAQALEARWTKDQILEAYLNLAPFRGDLQGVHAAARA
                     FFGKTPGELNRPESLILAVLLRGPNARPEVVGHRACMLAERLGTPAACASAQRLAQST
                     LDRQQLQPRWDLAPHAAAQLLHQPGERIVSTLDADAQKLVLAALRRSGVSEAAAVVLD
                     NASGDVIAYVGALEPAAADALPVPRTSASLLAPLVYGLALERRVLTAATLLEDALSNH
                     APTLPDEKLWVSARRALADGLPEPAAAVQRLVAEALPERLRVMELDPARGPAPDLSLL
                     QLAGFYRSLANGGLWQEARLTPGERAAPRRLLRSEANFIVGDMLVLDDAEGVPQPFVQ
                     RMASGDGRDMEVAGFAERYTLVLWAAGGRWTASSAGAAWQEILRGLYRGTVPARTVAA
                     PVGVVSSVVVFDPPVEPARREWFIRGTETDKVGMPRPAAHILHPAAGSLVDGMALADD
                     PAFRIRFEASPALPGLSWRLDGERIGRGAHLAWTPVAGLHTLELLAADDRVLDTVTFA
                     VRAPLP"
     gene            complement(20608..21855)
                     /locus_tag="PKX01_05915"
     CDS             complement(20608..21855)
                     /locus_tag="PKX01_05915"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HMZ75695.1"
                     /translation="MKVYCVGGAIRDELLGLPVQDRDWVVVGATPEDLLARGFRPVGK
                     DFPVFLHPASHEEYALARTERKTAPGYAGFVFHTDPSVSLEEDLARRDLTINAMARDE
                     AGTLMDPYGGQADLAARVFRHVSPAFAEDPVRILRLARFAARFADFRVAPETLVLMRR
                     MVDDGEVDALVPERVWQELARGLMEARPSRMLRVLRDCGALARLLPEVDRLFGVPQPA
                     KHHPEVDTGEHLCLVLDHAAATGQPLPVRFACLLHDLGKGVTPAPVLPHHYGHEASSE
                     RLARAVCERLKCPVDCRDLALMTAREHGIVHRALELRPDTTVKLIERCDGLRRPERFV
                     ALLDACACDHAGRAGLAGRPYPARDRLLAALAAVRAVDAGAIARQQADKARIPAAVHA
                     ARIDAVKALGRGTEVPVGAANDT"
     gene            complement(21852..22805)
                     /locus_tag="PKX01_05920"
     CDS             complement(21852..22805)
                     /locus_tag="PKX01_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HMZ75696.1"
                     /translation="MTSRKVLIVGGSGFVGTAVAARLARDGLSLRIPTRRRARVRHLL
                     TLPDVDLVEADVFDPPTLAGLMQGVDAVVSLVGVLHSRPGQPYGPDFARAHVELPTLL
                     VAAARQAGVTRLVHVSALGAAPDGPSEYQRSKAAGEAAIRAGAPELAWTILRPSVIFG
                     PGDSFLNLFAGLVRTFPVLPLGGAGARFQPVYVQDVADVVAEALVRPDAAGLTCDLAG
                     PTVYTLAELVAYVARLTGRSTRIVPLSEPLALLQAAVLERLPGPLMSRDNVRSMRADN
                     ITGGPPQPWGRTPTALETVVPRWLGRANRRGHFDAFRKPRP"
     gene            complement(22882..24849)
                     /locus_tag="PKX01_05925"
     CDS             complement(22882..24849)
                     /locus_tag="PKX01_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ75697.1"
                     /translation="MKNSVRAAIAAVLWAVLAAQADTGDERILAARDAARSGDRARLA
                     AYAAPAGHVLDPYPEYWWLSAQVARPADIDPAALREFLERNRGAWLAEKLRGEWLRGL
                     GRRGEWGTFAIEYPFMEQPEQDLQCYQFQARLANGDGMALAEARPLWFNLVDTPESCV
                     PVLQTLVREALVSPDEIWARVRRLMEAKRNTHARYAASWLPADQVPAPSDLDKAAANP
                     AGYLDRLPPNFSATRPGRELALIALARLARDDTLGAYVRYQRMDERFSPAERGYALGQ
                     IAWQAAQRLMPEAHTWYRAAGDTPMSDEQIAWRARAALRAGDWKGVRSAIEAMPASLR
                     EQPDWAYWLGRAQAALGKQDAARRLFEQIGGHPAFYSILADEELGRSFVLPKKARGPS
                     DDEATLVRTTPGVQRAVALFRLDMRTEGTREWNWSMRNRDDRFLLAAAAYAQELGIYD
                     RAINAADRTRSEHDYSLRYLAPYRERIEPNARSRDLDTAWVYGLMRQESRFVTAARSG
                     VGAQGLMQIMPSTGQWIAGKLGMKGYNIGWLRDPDTNVMLGTTYMRMVLEGLDNHPVL
                     ASAAYNAGPGRARKWRDSKPLEGAIYAETIPFNETRDYVKKVMANSIVYAALLDGRAP
                     SLKARLGTVLPGNGDASGLGSPAPGAGLENE"
     gene            24892..25503
                     /locus_tag="PKX01_05930"
     CDS             24892..25503
                     /locus_tag="PKX01_05930"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HMZ75698.1"
                     /translation="MTQPPSPPPAPDGTAERRRALRSARIAARQALAPAQRSAATARI
                     EAALDGLLAELAPRCLGFCWPWQGEVDLLPWMARWLAADPARQAALPVVVEAAAAMGF
                     RRWTPDTPMTADRHGIPVPATDAPVQPDVLLVPLNVFDGAGFRLGYGGGYFDRTLAAL
                     RPAPTGIGVAFELARADTVWPQPHDLPMDWIVTEAGAFRPRRG"
     gene            complement(25531..26448)
                     /locus_tag="PKX01_05935"
     CDS             complement(25531..26448)
                     /locus_tag="PKX01_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HMZ75699.1"
                     /translation="MTLTDLRYLVALARERHFGHAAERCHVSQPTLSVAIKKLEDELA
                     VTLFERSAADVKITDIGARIVAQAERVLLEAGQVKEIATAGRNPLAGPLRLGVIYTVG
                     PYLLPDLIPRVHAAAPQMPLLIQENYTAKLVDALKRGELDVIVIALPFEEHGIVTQAV
                     YDEPFRVLIPADHAWSAEQTIAAQQLAEAQLLLLGAGNCFRDQVLEVCPMCRNGDGLQ
                     RTLEGSSLETIRHMVATGAGITVLPSTAADRIPATNTLLAVRPFADPEPSRRIALAWR
                     VTYPRSGAIDVIRAAILGSHLPGVRPVGH"
     gene            26756..28255
                     /locus_tag="PKX01_05940"
     CDS             26756..28255
                     /locus_tag="PKX01_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HMZ75700.1"
                     /translation="MKSAVLRYSLAASAIAVALAGCLPEGAAISSSHAANATSATAPA
                     VPAAATTTAGRYGLPDFSALVEQVGPAVVNISVTQKRTLSNGDDFSGDPFDELLRRFG
                     IPGMPGMPGGNGGGRAPQQIQQGVGSGFIVSGDGYVLTNAHVVDGATEVTVRLTDKRE
                     FKAKVVGVDKRTDVALIKIDASGLPTVKTGDAEHARVGEWVVAVGSPFGFENTVTAGI
                     ISAKARRLPDENYVPFIQTDVAINPGNSGGPLFNLAGEVIGINSQIYSRSGGFMGISF
                     AIPIDVAMKVKDQLQRYGKVSRGRLGVSIQGLDPDLAQNFGLDKPAGALIANVESGSP
                     AEKAGLQSGDVVLGVNGQKVENSADLPRIIGEQKPGTSVRLSIWRDRKAREVSVTLGE
                     QLAEGAQPLGGFERKNEGASGKLGLTGRSLTAAEASRLGVAGGLLVEGVNGPAAKAGM
                     QPGDIILGLNNQPVTSVDQFRKLLEGAGNRFALLVQRGNSRIFLPIRMG"
     gene            28393..30405
                     /locus_tag="PKX01_05945"
     CDS             28393..30405
                     /locus_tag="PKX01_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ75701.1"
                     /translation="MSLFRPPAGVLLALAGLVSGAAVHASPEESPFFEDMPTVLSASR
                     LPEPLHEAPGAVTVLDRDFIRATGYRDVARLLRLVPGMQVGQERGHAQWVTYHGLGSD
                     YPTEIQVLIDGRSVFAPSSFGGVNWSGLPLTVEEIERIEVVRGTNSNAYGANAFLGVV
                     NIITRHSRQDPGGHVEANAGTHRIGDLAAAWAGGDERIGVRLSAAALNDDGFGSLFDG
                     RHTGLVSLRSDLRASTRDELTLRAGFADQRRDLGYPDSVFGNNAQRTSTTHNGTLHLI
                     WRHTDGPDEEWMASLYRNHESGVDRWNASAPPAYPSVPLDRNVSSMRTNAEFQHRFAL
                     GPSTRLVWGLEGRRDETDADTLFAGLRPPGFELYRAFSNLAWHATPAWQINLGALLEK
                     SPDTAAQFIPRAFVNWQASATDTFRAGYARAWEQRNLFDRYGDVRAYDPVSRALLAWP
                     YMPNPAARVPKVDTLELGYLGRFRALDATVDVRLFNERITDFVIREVIDPPMPVALPL
                     RTSRFVNLPTPVVLRGVETQWRLRPARDTELLINHTLIDRRADDATVTKRTAPYVASL
                     TWLQQYGGGWSSTASLLRMGPLAGGYGFVPGCDYAARAYTTVDLRLARRFTRGGRQIE
                     VALNGINLGAPHQEIADRSEQCLPAHLGTPVNPASPMGWLSLAVAL"
     gene            complement(30413..30751)
                     /locus_tag="PKX01_05950"
     CDS             complement(30413..30751)
                     /locus_tag="PKX01_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMZ75702.1"
                     /translation="MKEIRAVIRPQKLPRLREVLRAVPGFPGMTLSKAAGCGATVRHV
                     PASIREELTDFTEKVRVEIVAPDDLVDALVEAIVQVASTGQTGDGLVWVTSIEHAVFI
                     RKTVGPPEGT"
     gene            complement(30809..>33324)
                     /locus_tag="PKX01_05955"
     CDS             complement(30809..>33324)
                     /locus_tag="PKX01_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ75703.1"
                     /translation="LVNPDRLRHYQLTVRDVYEAVARNNANSGGGVLPQYAEQYLIRG
                     VGLVKSLSDIGAIVLKEIGGTPVFVRDVAEVSFGHEVRQGAVVKNGSTEAVGGVVMML
                     RGGNAKEVVSRIKARVAEINAKGMLPDGLRIVPYYDRSDLVDAALWTVSKVLIEGIVL
                     VVIVLFLFLGDVRSSLIVVATLVLTPLVTFMVMNRYHLSANLMSLGGLAIAIGLMVDG
                     SVVVVENAFARLGHARSGESRARIIFDAVKEVATPVIFGVGIIILVFLPLMTLQGMEG
                     KMFAPLAYTIAIALAVSLVLSLTLTPVLSSYLLKGGAEHDTWVIARLKAPYLKMLHWA
                     VAHSRATALGGVVLLVATLAMFPLLGTAFIPEMKEGSLVPGINRVPNISLDESVKMEM
                     QAMRRVMEVPGVRSAISGVGRGESPADPQGQNESTPIVSLKPRDEWPAGWTQDDIADA
                     IRDALQELPGVQVVMAQPISDRVDEMVTGVRSDVAIKVFGDDLESLRLKAEEIARVAG
                     GITGATDIRVERVSGQQYLSIEIDRAAIARHGLNVSDVNDVIEASIGGRQATEIYEGE
                     RRFAAVVRLPEAFRDNAEKIGKILVSAPGGVQVALENLATIRIADGPAQISRELAKRR
                     VVVAINVKDRDLGGFVAELQKTVGAQVQLPDGYYLEWGGQFQNLERAMGHLTIIIPVT
                     VGAIFFLLFLLFGSLKLASLIILVLPFASVGGVIGLLVTGEYLSVPASVGFIALWGIA
                     VLNGVVLVSYIRHLRADGMPPAEAVVHGAVMRFRPVMMTATVALLALVPFLFASGPGA
                     EVQRPLAIVVIGGLITSTLLTLVVLPTLYKWFDDTPYEA"
BASE COUNT         5217 a        11265 c        11898 g         4844 t
ORIGIN      
        1 gccgcccagt tcgagaagtg gtccctgccc ctggcggtga tcatggcggt gcccttcgcc
       61 atgttcggcg ccctcagcgc ggtgctgatg cgcagcatgc ccaacgacat ctacttccag
      121 atcggtctgg tggtgctggt gggtctggcg gccaagaacg cgatcctgat cgtggagttc
      181 gccgcccaga agctggacga gggcatgggc gtggtggacg cagcagtcga ggcggcgcgc
      241 ctgcgcttcc gccccatcgt gatgacgtcc ctcgccttcg tgctcggcgt actgcccctg
      301 gccctgtcca ccggcgccgg tgccgcggcg cggcgttcca tgggcaccgg cgtggtgggc
      361 ggcatgctcg ccgccacctt catcgccacc atcttcgtcc ccctgttctt caagtggctg
      421 tcccgcaagg gcaagggcag cggacgctcg accccgcccg ccactgccgc caccgaggag
      481 gtgaagtcat gagtcccgcc cgtccgaccc cggccctcct ggccgtgctc ctcgccgccg
      541 ccctgggcgg ctgcgcggtg ggccccgact acgtgcgccc cgagtccgcg acgaacctgc
      601 cggcccagtt cgcgccgggc agcgccaagc tcgcctacag cccgagcgtc gccagcacct
      661 ggtggaccct cttcggcgac cgccagctcg acgccctggt gggcaaggcc ctggccaaca
      721 gtccggacgc cctcaccgcc gccgcgcggg tggaagaagc cgacgcggtg atccgccagg
      781 tggacgcggc cctgctgccc caggtggacg ccaccgccgc cggcacccac agccgcatca
      841 gccagaccac cgccacgccg ctgccgtcca gcgtcgccgc ggtgcgcaac aacgcccgcc
      901 tggccctcac cacgtccttc gaactggacg tctggggcaa gctgcggcgc gcctccgagg
      961 cggcccgcgc ccaggcgctg ggcagccgct acggccgcga cacggtggcc ctgtccatcg
     1021 ccgggctggt cacccagggc tatctgaacc tgcgcgccct cgacgcccag cgcctcgtca
     1081 ccgccgaatc ggtggccagc gccgagcgca gcctcaagct cacccaggtg cgcttcgacg
     1141 ggggcatcgc gtcgcagctg gacgtgcagc aggccgactc cgccctcgcc accctgcagg
     1201 cggcgctgct ccagctcgat caggcccgcg aacaggccga aaacctgctc ggctacctgg
     1261 tgggcgaacc aggtctgaag atcgctgccg gcgatctgcg cgacctgccg atcccgcccg
     1321 tgccgcccgc cggcctgccg tccagcctgc tggaagcccg ccccgacgtg cgccaggcgg
     1381 agaccgacct gatcgccgcc aatgccaaga tcggcgtcgc caaggccgcc ctcttccccg
     1441 gcatcaccct gaccggcaac ttcggccgcg agagcaagga cctgtcccag ctgttcacgt
     1501 cgccgtcgtc ggtctgggcg ctgggcgccg gcatcaccca gccgatcttc gaaggcggcc
     1561 gcctgcgcgc ccaggtggat caggtcacgg cccagcagaa gcaggtcgcc gaggcctatc
     1621 gcaaggcggt atacaccgcc ttcaaggaga ccaacgacgc cctggcgagc ctgcgcgtgg
     1681 gtgccgatgc ggaagacacc ctggcccgtg ccctgaacgc ggccaagcgg aacctgatgc
     1741 tggcccaggc ccgctacgac ggtggctact cgccgtatct ggaagtgctg accgcccagc
     1801 gcagcgccaa cgacgccgcg ctggcgtgga tccagaaccg ccaggcccgc ctggcagcgt
     1861 ccgtggatct gttccgcgcc ctcggcggcg gctggcagga cagccagaaa ctcgcggcga
     1921 acccctgacc cgccgtccgg ggcggttccg gtcgatgcag cgggcgcctt cgggcgcccg
     1981 ttgtgcttgc gccctcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     2101 ttccggtcga tgcagcgggc gccttcgggc gcccgttgtg cttgcgccct caggggtggg
     2161 gcgaagccgc ggcgcaaggc ggtcgccggc ccggtgcagg cagacgccgc gaccgacgac
     2221 gggctggatc gtcggacgtc ctcaggcgga ctcaggccgc ctggccatca tgtccggaac
     2281 cgatggcagc gcggtccggg atgctccgca gggcgtcctg gcccgctcag ggcgcggggg
     2341 ccttgctgcg gacggcgcgg gcctggcgcg cctcccactc gtccaccggc atcacggtga
     2401 cgcgcacctc gggcgcctgg gcctggccgc cgaggatcac cccgcgcagg ggcgacacgt
     2461 cctggaagtc ccgcccccag ccgagggtga catggcgcag gtcgggctgg atgccgttgg
     2521 tggggtcgta ttccacccag ccgttctcgg gacaccacac cgccagccag gcgtgggagg
     2581 cgtcggcacc gaccaggcgg ggcttgcccg ggggcggatc ggtgagcagg tagccggaca
     2641 tgtagcgcgc cggcagcccg accgagcgca gggcggcgat catcaggtgg gcgaagtcct
     2701 ggcacacgcc gcggcggcgc tccagcacct cgaacaccgg ggtgctcact tcggtggcct
     2761 tggcgtcgaa ggtgaaggtc ttgtagatga tctccatcag cgcctgcaca ccggccagca
     2821 gcggcgtttc cggcttgaag gcaagggccg caaaggcggc caggcgggca tcccgtgggg
     2881 cgcgcggcga gctgtagaga tagcgctggg catcgaggga gtccgcatcc cgcgcaccgg
     2941 agtggtaatg gaagatctcc cgcacggatt cccaggacgg cgactcctcg gccttgggca
     3001 gcgtgcggcc cagcaccgtg acgcggctct cggcccgcac cagcagaccg tcgtggggcg
     3061 tgtcgagggc catccaggcc accggattgc cgaagacgtc ctcaccctgc tggatgcggg
     3121 cgagatccgg atcgacggtg agctcggcct tctccacggt ctgccacggc agggcgcggg
     3181 gcatcaggtg gagcaggtgc tgggcgaggg acacgggcac gccacagggc gtgtattcgg
     3241 tctcgtggag gacgatgtag cgggcgctgc gagtcatgat caggcggcga aggtggcctg
     3301 gctgccgcgg ccgacgtggc tgaagaagcg catggcaagg cggtcggaca gttcgcggcc
     3361 ggcatgcacc agcgcgtcga gggcgtgggc cagggccagg ctggccgctg cccgctcggc
     3421 gtctgggcgg acgcgctcga ggctctccca ggagaaatgc tcgatgacgt acagggcctc
     3481 ctccagcccg gcgatgccga tgccgtcgcc gtcctgctcg tacttgccca ggtagttgat
     3541 gatcatgcgc acctggaagg ccagcgcgtg ggggttggcg tcgtccatca ccagcaggtg
     3601 gatggccggc agcagctccg gctgggcgcg atagcgggtg cggtaggtga tgatggagtc
     3661 gcacagctcc agcagcgcct ccacggccat ctcgcgctgc agcggctggg cggtgtcggc
     3721 gtgacagcgc aggaagtggg caagggtgct gcccaggctg tcgagacgtt cgatgcggcg
     3781 cccgaggatg tgcagacacc agcccatgtc gcgggtcatg ttgtccatcg cgaagccgga
     3841 cagggacacg aaggtggtga gggcttcgtc gagaaattcc agcagcgccg aggtgtcgct
     3901 gacccggccg cgacaatcct cgatctcggc ggacaggcgc ttgagggcgt gccagtggtc
     3961 gagggagagg tgttcccgcg cctgggtggc gcaccaggtg aggcgttgca ggttggcggc
     4021 gaggccgttg ccggcgcgtc ggtcgagcac cgcgcggaac agggcgagct cggccttgcc
     4081 gagggaggtg cggaccacgt cccgctccac cagttcggca ccggcggcaa gctccagcag
     4141 catggcgagg gcggcggaac gctcggtacg ccgcccttcg gacaggcgcg ccagggtcag
     4201 gcgggcgagg cgggccacgg cgtcgcagcg ctcgctgtag cgcccgaacc agaacaggtt
     4261 ctccgccagc cgcgacgaca gcgtgccggt gaccttgacg agatcggcca cccgcaggtt
     4321 cttgggcagc atgctgaagg tggacacggg gccatcggac agcacccagg tgtccttgct
     4381 gctgccgccc ttctgcatgg tgagcaggcg ggcgttctcc atgccggcca cccgcgtcag
     4441 gccgccgggc agcaccgtgt agccttccgg cgacgcggcc gcgtagaccc gcaggcccat
     4501 ggagcggttg atgaggcgac gccgatggcc gcctgcccag gccggtgcgc cggacagctg
     4561 gaccagctcc tgggcgaccc aggccgacgg attggcggcg atgcgcacgc gcagggcctc
     4621 gcgctggtcc tcgtcaagct ggtcgccgaa gaccacctcg ctcaggcccc cggggaaggt
     4681 gggcttgatg accagggagt cgaggtggcc gagcacatgg tccagggccg gatcctcacc
     4741 gcaccaccag gtggcgaccg ccggcatggc cagctcttcc ttgagcaggt ggcggcagat
     4801 gccgggcagg aaaccgggca gcgcggcgga ttcgagcagg ccggagccga gggcattggc
     4861 caccaggaca cgcccggcac gcacggcgcc gagcaggccg ggcaccccga gggccgagtc
     4921 ggcgcgcagt tcgaccggat cgcagaaatc gtcgtcgagg cggcgcagga tggcgtggac
     4981 gcgcttcagg ccggagacgg tcttgaggta aacggtgtcc tcacgcacgg tgaggtcctg
     5041 cccttccacc agcgggaagc ccaggtagcg ggccaggtag acgtgttcga aataggtttc
     5101 gttaaagggc cccggcgtga ggaggacggt gaggggcacc tggttgccgt cgcacggcgc
     5161 gtgggccgcg agactgcgct gcaggctgcg gaagaaggcg gcaagggtct gtacctggag
     5221 gtcgcggaac agatccggaa aggcgcggga taccagcagg cggttctcga gagcatagcc
     5281 ggcgccggaa ggggattggg tgcgatcggc gatgacccac cagcgcccat ccggcgagcg
     5341 ggccagatcc acggcgtaca gatgcagata ggtgccgcca ggcggctgga tgccctggca
     5401 gggccacagg aaacccttgt gaccatagac cagctcgggg ggcagcaggc cctcggcgag
     5461 ggtggtctgg gggccataga ggtcggccag cagggcattg aggagggtgg cccgctgggc
     5521 gacggccgtg gagatctcgc gccattcatc ggccggcagg atcagcggca ggaggtcgag
     5581 ggaccagggc cggtccgtgc cgcgcgcgtc cgcatagacg ttgtaggtga cgccgttctg
     5641 ctggatacca cgcgccagct gggcggcgcg ggccttcagc gtgtcggcgc tggactcgtc
     5701 cacgtggtcg aggaagtcct gccagtgggg acgtacgccg tccgggccga acagctcatc
     5761 gtaagcgtcg gtgcgatacg ggtaatgact gagaatcgaa ctgggcatgt agcaacgcta
     5821 acactgttcc ccgtatccgg gaaatgaaca atttcggggc gacccgcaga cgacaggcgc
     5881 gcaccccgcc gccccgtcgt ctgggaccgc ccgtcgtccg gccgcaagcc cggacgacgg
     5941 gcagttcgga tcagcgccgc aggtcgagcg tgaaggggaa ttccttctcc acggccgagg
     6001 tctcgaactt cggtgcgccc ggcgtgtgcc ccatgcggaa gaagcgcgcc agccggcgac
     6061 tttccgcctc gaaggcattg atcgggaagg tctcgaagct gcgcccgccg gggtgcgaca
     6121 cgtggtactg gcagccgccg agggaacggc cggtccaggt atccaccaga tcgaacacca
     6181 ggggtgcgtg ggacgggatg gtgggatgca ggcaggacgg cggctgccag gcacggtagc
     6241 gcacgccggc cacgaactca cccacggtgc cggtgggctg cagcgggatc gagcggccgt
     6301 tggcggtgag gaggtagcgg tcgtcggaca tgccgctgac cttcacctgc atgcgttcca
     6361 ccgacgaatc gacaaaacgc acggtgccgc cgatggcccc ttcctcgccc atcacgtgcc
     6421 agggctcgag ggcgccccgc agttccactt cgatgccacg ggcggcgaag tcgccgatct
     6481 tggggaagcg gaattcctgg tggggcgcga accactccgg cttgaagtcg aggccgtgac
     6541 cctgcatgtc ctcgatgacg tcgctgaagt cctgccacac gaagtggggg agcaggaagc
     6601 ggtcgtgcag ctcggtaccc cagcgcacga ggcgctcggg cgcgtagggc gtgtcccaga
     6661 agcgggccag caggccgcgc aggaggagct gctgggccag ggacatgcgc gcgtggggcg
     6721 gcatctcgaa ggcgcgcatc tccaccagcc ccaggcggcc ggcggggccg tcgggcgagt
     6781 agagcttgtc gatgcagaac tcggcccggt gggtgttgcc ggtgacgtcg atgagcaggt
     6841 tgcgcagcat ccggtcgatg agccagggcg ggacgctgtc gccgaccttg gggaactggc
     6901 ggaaggcggt ctcgatttcg tagacggaat cgttgcgtgc ctcgtcgatc cgcggggcct
     6961 ggctggtcgg tccgatgaac aggcccgaga acaggtagga caggctgggg tggttgtgcc
     7021 agtaggagat caggctgcgc agcaggtcag gccgccgcag gaagggcgag tcggacggcg
     7081 tggcgccgcc cagcacgaag tggttgccgc cgccggtgcc ggtgtggcga ccatccacca
     7141 tgaacttctc ggtggtcaga cgggagaagt gggccgcgtc gtagagatgg gtggtgcggt
     7201 ccaccagctc ttcccaggag ccggccgggt ggatgttcac ctcgatcacg ccggggtcgg
     7261 gcgtgatgcg gaagtgggtc aggcgcggat cggtgggcgg ctcgtagcct tccaggacca
     7321 ccggcgtgcc gaaggcctcg gccgagctct ccactgcggc gacgagttcc agatagttct
     7381 ccaggcgcga caggggcggc atgaagatgt gcagggtgcc gttgcgcggc tccacgcaca
     7441 tggcggtgcg cacgatccag tcggcagact cgaaggactg cggcgtgcgg tcggtggggc
     7501 tgagcttctc ggcggcccgg gcggccttga gggcggcggc acgggcggca gcgccgctgc
     7561 cgaggccgta ggtctcgtcg tccttgaagg cattgagggc gttgagcacc gccgtctggc
     7621 cgccgggcag ctgacggcgg atggcctggt attcaggcag cggcgggaag ctctggtggg
     7681 cgtccggcgg attgacgtag gggtagctgg tggccgtcac ccagggctgg gaatccagcg
     7741 gcagacggta gccgatcggg gaatcgccgg ggatcaggta gcagcgctcg tcccgcagga
     7801 accacggccc ggtctgccag gcgccggtgt aggtgttgat ggagatgggc agcacgtagc
     7861 ccacggcgtt ggcaaggccg tagtcgaaga cgcggcgcag gcggtcgcgc tccagcggat
     7921 cgtccaggcg ggcgtcgaag ggatccacgt tgcccggcag gcggcgctcg cgccacaggt
     7981 agtagaaggc atcctcgtag ccggcgaaga cgcggcgtgc gtcgaggccg aggcgctccg
     8041 ccacgtcccg caggaaggcg ccggccaggt cggcgtcggc gtcacccggc gtggcggaat
     8101 cggcgatgag gtccggatgg gtccagatcg gctcgccgtc cttgcgccag tagcagttga
     8161 gggaccagcg cgggagctgc tctccgggat accacttgcc ctggccgaag tgggccaggc
     8221 ccgtggggcc gtacttgtcg cgcagacggt ggtagaggtc ttcggcgcgg acgcgcttgg
     8281 tggggcccat ggcctcggtg ttccattcgg cgccgtccgg gtcgtcgatg gacacgaagg
     8341 tgggctcgcc ccccatggtg aggcgcatgt cgtgggcctt gaggtcttcg tcaatgacat
     8401 gacccagggc ttcgatctcg gcccattgtt cctcggtata gggcttggtg acgcgcggcg
     8461 cttcgagcac gcgggtgacg ctcatgtggt gggcgaactc gcactcgcat tcgtccacca
     8521 gaccggtgat cggcgccgcg gacgtgggat cgggcgaggc ggccagcgga atgtggcctt
     8581 cgccggcgaa gaggccggag gtgggatcga gacccaccca gccggcgccg gggagataca
     8641 cctcggtcca tgcgtgcagg tcggtgaagt ccacctcggt gccggacggg ccgtcgaggg
     8701 ccttcacgtc ggccttcagc tggatcaggt agccggacac gaagcgcgcg gcgaaaccca
     8761 ggtgacgcaa cagctggacc agcagccagg cggagtcgcg gcacgagccg gtgcgctttt
     8821 cgagggtttc ttccggtgcc tgcaccccgg gctccatgcg gatcacgtag ccgatgtgct
     8881 gctggagctt ggcgttgata tccaccagga aatccaccac gcgacggctg tcccgcggca
     8941 cgctgtccag gtaggcctgg aacaggggcg tgggcgcgtc cttgaggcgg aagggcgcca
     9001 gctcatggct cagattgccg gcgtactcga agggatagtt ctcggcttcg ggctcgagga
     9061 agaaatcgaa ggggttgatg accgacatct cggccaccag atccacctcc acggtgaatt
     9121 cggtgacttt ttccggaaag accagacggg ccagatagtt ggactgggga tcctgctgcc
     9181 agttgatgaa gtggggcttg ggcagcacct tcagcgagta ggcaaggatg cgggtgcggc
     9241 tgtggggcgc cgggcgcaga cggacgacct ggggtccgag ggtgacgggg cggtcgtatt
     9301 tataggaggt aaggtgattg agggcgacgt gaatggtcac gggatactcc gatctctggt
     9361 tcgcggggtg aaggcgcgcg gggcttgccg gcagttttag caagcttcac gccagaagat
     9421 ttacccgcag caggttaagg ttttaccggc gatacagggg cttgcagagg gtgcactagg
     9481 cagggcgggc aatttcgagc ctcgccttgg ggcacgcacc agaatggaca aggccggacg
     9541 caccgcgctg gtgcgcccgg ctggatgcga cggatggggg gaaacaagat cctttgcctc
     9601 agcggcgtcc gcccaggatg gagcccagca caccccggac gatctcccgg ccgacctggg
     9661 aaccgatggt gcggacgacg ctgcgggcgg cggtttcggc gaggccgggg tgccggccgc
     9721 cgcgagggcc ggtggtgccg aagaggatgt caccgagccc accgagcaga ccgcccccat
     9781 cctgggccgg tgccggcgcg gacgccccgc cgggggctcc ggccggtgcc gcctgggccg
     9841 ccggagcgcc acgcaatttc tcgaacgccg actcgcgatc cacgatctgc tcgtagtggc
     9901 cgaagatcac tgaattttcg atcgcggcct tgcgttcctc gccgctgagg ggccccaggc
     9961 gcgacgcggg cgggacgatg aagccccgct cgacgatgga cggccggccc ttctcgtcga
    10021 gaaaggatac cagcgcctcg ccgacgccca gttccatgat caccgtggcg gcgtcgaact
    10081 cggggttggc gcgcagggtc tcggcggcgg tcttgacggc cttctggtcc ttcgggctga
    10141 aggcccgcag ggcgtgctgg acccggttgc cgagctggcc gaggacgttt tccgggatgt
    10201 ccagcggact ctgggtgacg aaatagacgc cgacgccctt ggagcggatc aggcgcacca
    10261 cctgctcgat cttctccagc agggccttgg gcgcctcgtt gaagagcagg tgggcctcat
    10321 cgaagaagaa caccagcttg ggtttgtcca gatcgcccac ttccggcaga tgctcgaaga
    10381 gctcggccag cagccacagc aggaaggtgg agtagagctt gggcgaattg tagagcgcgt
    10441 cggcggcgag gatgttgacg acaccgcggc cgtcgccgtc ggtctggatc aggtcggcga
    10501 tgtcgagcat cggctcgccg aagaacttgt cgccgccctg cgcctcgatg gtcatcaggc
    10561 cgcgctggat ggcgccgatg gaggctgcgg agatgttgcc gtactgggta gtgaagtcct
    10621 tggcgttgtc gcccacgtac tgcagggtgg cgcgtagatc cttcaggtcg atgatcagca
    10681 ggccctggtc gtcggcaatc ttgaaaacca gttgcagcac gccggcctgg gtgtcgttga
    10741 gattgagcag gcgcgccagc agcagcggcc ccatgtcaga gacggtggcg cgcaccgggt
    10801 gacccgaggt gccgaagacg tcccagaaca ccgcggtatt ggcgcgcggc gtgaaatcgt
    10861 cgatgcccag ggcggtgagg cgctccttca gcttcggtga cacgttgctt gctgcaccga
    10921 tgcccgacag atcgcccttc acgtcggcca tgaagaccgg cacgccgatg gccgcgaagg
    10981 actcggccat cttctgcagg gtgacggtct tgcccgtgcc ggtggcgccg gtgatcagac
    11041 cgtgacgatt ggcaagggcc ggcaggagga cgatgtcctt ggggccggac ttggcgatga
    11101 ggagcggctc ggtcatgggg atctccaggc gggcgaaagg gggcggactg cgccaatcca
    11161 gcaattatcg gtcaccccgc gggattttag ggagcgcctc tgcgtgccgc ggcacggaat
    11221 gaccgcggaa ccggcatcgc cgggccctgc gggaggctgc gcgaagggct gccggcgcgg
    11281 taaaatcctg cctttcgggt gcactttcaa ggacacagaa tcatggcggg tcacagcaaa
    11341 tgggccaaca tccagcatcg caagggtcgg caggatgaaa agcgcggccg ggtcttctcc
    11401 cggctggcca aggaaatcac cgtcgcggcc cgcatgggcg gcggcgaccc gggcttcaac
    11461 ccgcgcctgc gcctcgcggt ggaccgggcc aaggccgtca acatgcccgc cgacaacatc
    11521 gaccgggccg tcaagaaggg caccggcgag ctggaaggtg tcacctacga ggaggcccgc
    11581 tacgaaggct acggtatcgg cggcgccgcg gtgatggtcg attgcctcac cgacaacaag
    11641 acccgcaccg tggccgacgt gcgtcacgcc ttcaacaagt tcggcggcaa catgggcacc
    11701 gacggctgcg tggcttttca gttcaagcac tgtggccagc tgctgttcgc gccgggtacc
    11761 tcggaggatg cgctgatgga agcggccctc gaggcgggcg ccgacgatgt ggtgagcaat
    11821 gaggacggct ccatcgaggt gctcaccgag ccctaccagt tcggcgaggt caaggacgct
    11881 ctggaaaagg ccggcttcac gccggaattc ggcgaagtga cgatgaagcc cctcaacgag
    11941 gccgaactca ccggcgatga cgcggtacgc atgcagaaac tgctcgacgc gctggaatcc
    12001 ctcgacgacg tgcaggcagt ctacacctct gccgtcatgg acgacgcctg atcgcacgcg
    12061 cgccgttgcc gccccgatgt gatggacggg gtggttcggc ggcgcgggag cgggctccgc
    12121 tgcaaggcgc cgcgcgcctc agcccagggc gaaggcgacc gcgccgctca tgttgtcggc
    12181 aaccgagaac ccgcagccga tatagccttt ttcgccaagg atgaagtcgt cgcgcttgat
    12241 gcagtgctcg gctgcgcctt cttccagcag gtaggtcccg ttggtggatg aatcggtcag
    12301 atagaacaga ccattgcgca gttcgatacg cgcatgctgg cgggacacac gcggatccac
    12361 gacgacgatg tcgttaccgg tctcacggcc gaagagcagg gtcggggaac tcgcgtcgac
    12421 cgtccagctg cgcccctgga agctgacctt catctgggcc ttgggccgca cgctggcgtg
    12481 gcgcagttcc acgggactgg gcggcgtggc ctcgcccagt tcgagcacct ggaagggcaa
    12541 ctggtcgttc gcctggacca tggacgagag gagcttgcgc atggccggcg acagggcttc
    12601 actgaaggcc tcggagacgg aaacggtgtc cgggttgtgc ccgacggcca gccgcgaggc
    12661 cagggtttcg gcccccttca gatcgtccgg cgcctcggat tcgagcacca ggcccgcgcg
    12721 cagaacgagc ttgatcccgc tcatcggtgg cagggcacgg acccgttcgc gcatctcgcg
    12781 ggcggcgtag agcgccgcgt cggcatccgc aaagcgcgcc accagcacgt ccggacgcac
    12841 ctcgatgccg gacgtggcat aggcttcagc gcagcgggac atgcggttca ggcagcgttc
    12901 gacagcgtac cctgcctccg catcgccgag cttggcggcc agccggacac cgccggccac
    12961 atcggcaaac aggatgcacg gttgatgtcc ggccccgccg gcgcccgtca cgacgcggga
    13021 cggacgggca tggacggatt cacgggacga cgaattctca gcaaaggcgg gggcgtgggt
    13081 catggtgact tctcacggag aaaccccaga ctgaccgcag cctccgcggg cgggacggag
    13141 ggcagttccg gggcatcgaa tacaagatct ccctgatcta ccttggtatc gcacgagaca
    13201 ttcaggaagt caaacagctt tccgtccgcc aggtgggacg ggacaacgtt ctgcaaggcc
    13261 gtggcgatgg acacgatgcg cccgggaaaa cgcgcatccc agtcccgcag catggccttg
    13321 atctgcttgc gctgggcgtt ctcctgggag ccgcacaggt tgcacgggat gatgggatag
    13381 cccatcccgc gggcgaagcg ctcgatgtct ttttccgggc agtaggccag cgggcggatc
    13441 accacgttct ggccatcgtc gctcaccagc ttgggcggca tggccttcat cttgccgccg
    13501 aagaacatgt tcaggaacag ggtctcgatc atgtcgtccc gatggtggcc gagggcgatc
    13561 ttggtggcgc cgagttcctt ggcggtgcgg tagatgatgc cgcggcgcag gcgcgagcac
    13621 agggagcagg tggtcttgcc ctcggggatc ttgtccttga cgatcttgta ggtgttctcg
    13681 gtgacgatgc ggtattcgac gccgatggac tcgaagtagg cgggcaggac gtggtccggg
    13741 aagcccggct gcttctggtc caggttcatc gccacgatgc ggaaattcac gggcgcccgc
    13801 ttctgcaggg ccagcaggat agacagcagc gagaaggagt ccttgccgcc cgagacgcag
    13861 accatgacca cgtcaccgtc ccggatcatc ccgtagtcgg cgatcgcctc gcccaccttg
    13921 cgttccagac gccgcttgag gttgcggaag gtgttggacg tgcgggattc cggcgtggga
    13981 gtgtcggtcg gaagggggag ggctgcgccc acggcgagat ccatgatgtc gtgatgtccg
    14041 attatagtat ggggatattc gggccttccc cggacgcaat gtaatttaac ttagaggtgc
    14101 gccgagcgcc ccccatccac ggcaatgatc tgccctgtga tgtaaggcgc attgcacagc
    14161 aaaaaaacca ccgtgcgtgc cacgtcgtcg ggctcaccga cgcgcccgag gagcgtgtgg
    14221 cggacgaccg catcctgcgc tgcgggcggg aactggccat cgtcgggcca ttcgatggcg
    14281 cccggcgcca ccccgttcac ccgcactgcc ggccccagct ccacggcaag cgcgcgggtc
    14341 aggccgagca gcccggcctt ggcggcgcag tacagtgcat agccatgcag gggccgttct
    14401 gcgtgcacgt caacgatatt cacgatgctt ccgcgggtcg ccagcagatg gggcgcagcc
    14461 gcctgggaca ggaacagggg cgccttcaga ttggagccga ccagatcggt ccaggcggct
    14521 tcgtccatac gccccatggg ggtcgggaag aaactcgatg cgttgttcac caggccgtcg
    14581 aggcggccga aacgggccac caccgcggac accagggcgg gcagcacggc cgtgtcgaga
    14641 agatcggcct gcagcaccgc cacggacccg ggccgccggg cgttgagctc ggccgccagc
    14701 gcctcggcct cgtcgcggga gtgccggcag tgcacggcca cccgcgcgcc ttccccatga
    14761 aggcgacgga cgatggcggc gccgacgcgc cgggctccgc cggtgacgag catcaccggc
    14821 gcgggggagg ctgaggacat gactgacgga aggaggcagc ggggatgaac ccgatatctt
    14881 acgggccgga cgcgacgccc gggcgatttt ctcggcccgg cggcccgacc gggcggcacg
    14941 ctaaaataac ggttttgtgc cccgactgtt gacatgaacc tgcccccgcc ttccccggat
    15001 gccctcgccc agagcgagcg cctcgttgcc cgcctgcgtg acctcatcgc ccgcgaaggg
    15061 ggctggatct ccttcgccac ctacatgggc gaagccctct acgcaccggg gctgggctat
    15121 tacagcggcg gctcgcgcaa gttcggtccg ggcggcgatt tcatcaccgc accggaactc
    15181 acgcccctct tcggccaggc cgtcgccggg cagctcgtgg agatcatggc ccagtcggcg
    15241 ccacagctca tcgaggccgg cgccggcacc ggcctgctgg cggccgacct gctcctcgaa
    15301 ctggaacatc gcggtgccct gccggagcgc tatgcgatcc tcgaagtgtc cggcgaactg
    15361 cgttcgcgcc agttcgacac cctggccgaa aaagtgcccc atctcgcctc gcgggtgtgt
    15421 tggctcgaca ccctgccgga gcacttttcc ggtgcgttga tcgccaatga ggtgctcgac
    15481 gtgatgcccg tgcatctggt ggcgtggcga cccgaggggc tcttcgagcg gggcgtggcg
    15541 atggatgacg caggccgctt cgtgtgggcc gacgtgcccg ccagcggccg cgtggccgaa
    15601 gccgccgctg cgctggagcg accgttgccg tccaccggcg agtacgtgag tgagctgaac
    15661 ctcgccggcc gggcgtggat cggcgaatgg gccgcgcgcc tcgacaaggg cgccctgctc
    15721 ctggtggatt acggctaccc gcgcgccgag tactacctgg acagccgcag caccggcacc
    15781 ctgctgtgct actaccgtca ccgcgcccac cccgatccct tcctgtggcc ggggctcaac
    15841 gacatcacgg ccttcgtgga tttcaccgcg atggcggagg cgggcttcga cgcaggcctc
    15901 gacgtgctgg gctacaccag ccaggcgacc ctgctgctca actgcggcgt gctggaatgt
    15961 ctggcgcggc gtgcgccgga ggacagcccg gactacatcc gggcgtcacg ggcggtgcaa
    16021 aggctcaccg gcccccacga gatgggggaa ctgttcaagg tcattgccct ggggcgcggc
    16081 atcgacggac cactcctcgg ctttcgccgc ggcgaccgcc tgcacacact ctgacccacg
    16141 gaggcggcac gccggggcaa tggcggcccc ggcctgcgtg ccgtgctcat tcctggaagc
    16201 ggtagatcgc gctcaggaag gcgtccacgt cggtgggcac gaaatgccgc tcggcgtggg
    16261 tgaccgccac ttcctgcaga cccagggcca gctcgggtgg caggaggttg gcgttgagga
    16321 tctcatcccc gtattccggc gcctgctcgt ccaccggatc catgcgcgcc tgggcgccgc
    16381 tttccatgtc catgtagatc accatttcag cctccgtcgg ggaagcgcac gatgcctccc
    16441 acatagttgc taacggctgt ccttcagaaa ggttgaaggc cgccccgtga gacaccgtgc
    16501 acgaaccgtg ccggtgtgac gcgacaagcc ggtgatgtca gcatggacga ggtcgccacg
    16561 ccgcgccagg acacctgtca cggccgacgc ccgatgccgc ttacagcccg tgggcggcca
    16621 gccaggcggc gatcggatcg gcggcgatgc gccagcccgc ctccagcatc atgccgtggc
    16681 ccaggcgcgg cacgatgtag gcctcggtgc cataacggcg ggcggtcatg cgcacctggt
    16741 cgggcgggat gagcatgtcg tgttcggctc ccaacaccag catgggcggc aatgcgacat
    16801 ggccgagggc gggcaggtcg aacagggaca tatcccagat cgcccggtgg gattccggct
    16861 ggcacaggtg gtaatactgg cgcaggcgca ccagctcgac cggttcgtgg aaaagggcgt
    16921 cccgcagggt gtccagatcc ggctggcccc cgcccatgat gcggttcagg tcgcccatca
    16981 tgttcggtcg cgaaaaagcc agcccgaagg ccgagcccag caacccctgg ggcggcaccg
    17041 cggccatcag caccgccgcc ggcacgcgct gccgctccag acacttctgc accaccatgc
    17101 cccccatgga atggccgatc agcaccggcg gcgcgggcag gcggtcggcc acgtcggcca
    17161 cgtcagccac gtaatcggcg agggaatagc tgtccagaaa ggcccggttc cggctgctcc
    17221 cgtgccccga cagggagacg gcgtacgccg catagcccag actcgcgaaa tggggcagga
    17281 agtgctcctc ccagcaccac gccgccgtga aggcaccatg gacaaagagc agcggcgtgg
    17341 gacggggcgt gccggacggc aggcgacaca ggacttccag gtcaccgaaa cgggcggatt
    17401 tcatcggggc tgttcttgtt gtggtgtcgt ccgcacggat ggcggttgcc gagaccggac
    17461 gacgcaagtg tggggcgtgg ggcgggaaca taccggaatc ctggggtggt gagaagcggt
    17521 ccaccgcggg cacgggcaat cggggagtgc gctgcacgca cgctcgaagc gtcctgcagc
    17581 gcatggtcgg accgagtgtg ccggaagaac cggcccgacg cgcggagaaa gaggacgtcc
    17641 ctggagatcc cggtacgccc cctcgattcc ggcgctattt gaattcgaaa cgaacgtcct
    17701 tgaccacgtg atgcttgccc caatggaaat tgcagacacg ggcaacttcc agcccctcct
    17761 tgctttcggg gcgacatcca ccgctgatct tgacgatttt ctccccctgg agcgagaggg
    17821 gaaactcgac caccagcccc tcgggaatct cgtgttccag ttcgaccttt tccgcaaaat
    17881 catcgacgag cgcaaagatc acctgccgat cggctttcaa cacgtagagg tcgtgctgga
    17941 gcgcggcgcc gggcacgaga cacaattcca gcgtcgattt ctcgacgatc tgctgcctgc
    18001 attggggtgg atccgcagcc agcgcaccgg acgcacacag tcccagcaat agcccttgca
    18061 cccaaggctt catcgtgtct tccatcgttg actccggcat ggcgaaaggc gccgtgcatg
    18121 cctcaggatg cccgatcccg cagcgaaatt atacgtcatg agcgcgtcca tgctgggcgc
    18181 ccgccgacgc attccgctct cgaaagtgct tccccgctca ggccggctac ggttcaatac
    18241 cgccttcgtt ctgcccgtcc cccgccccat gctcaaatgg ctgctcgcga tcgtcgtgat
    18301 cgccgtcgtc tccggtctcg gccgcggcat ttcgccgggg cgctggcgca tcggccacct
    18361 gccgggggat ctgcatttcc atctgtgggg gcgccacatc cacatcccct tcaccagcac
    18421 cctgctgctg tccctggcgg cgatgctggt gatccggctg ctttagggca ggggcgcgcg
    18481 gacagcgaag gtgaccgtgt cgaggacgcg gtcgtcggca gcgaggagtt cgagggtgtg
    18541 caggcccgcc accggcgtcc acgcgaggtg ggcgccgcga ccgatgcgct ccccgtcgag
    18601 gcgccaggac aggccgggca gcgccgggct cgcctcgaag cggatgcgga aggcgggatc
    18661 atcggcgagg gccatcccat ccaccaggct gccggcagcg ggatggagga tgtgcgcggc
    18721 cgggcggggc atgccgacct tgtccgtctc ggtcccgcgg atgaaccatt cccggcgcgc
    18781 cggctcgacc ggcggatcga agaccaccac cgacgacacc acaccgaccg gcgccgcgac
    18841 cgtgcgggcc ggcacggtgc cccggtacag gccgcgcagg atctcctgcc aggcggcgcc
    18901 cgcggaggag gcggtccacc gcccgcccgc ggcccacagg accagcgtgt agcgctcggc
    18961 gaagcccgcc acctccatgt cgcgcccgtc gccactggcc atgcgctgga caaaaggctg
    19021 aggcacgccc tccgcgtcgt cgaggaccag catgtcgccg acgatgaagt tcgcctcgga
    19081 cctgagcagg cgccgcggcg ccgcgcgctc acccggcgtc aggcgcgcct cctgccacag
    19141 accgccgttc gccaggctgc ggtagaagcc tgccagctgc agcaggctga ggtccggcgc
    19201 cgggccgcgg gccggatcca gctccatcac gcgcaggcgc tcgggcagcg cctcggccac
    19261 caggcgctgc accgccgccg ccggctcggg cagcccgtcg gccagggcac ggcgggcact
    19321 cacccacagc ttctcgtccg gcagggtggg cgcgtgattg gacagggcgt cctccagcaa
    19381 ggtcgcggcg gtcaggacgc gacgttccag cgcaagccca tagaccagcg gcgcgagcag
    19441 gctggccgac gtgcgcggga caggcagcgc gtcggccgcc gccggttcga gggcgcccac
    19501 ataggcgatc acgtcgccgc tggcgttgtc gagcaccacc gccgccgcct cgctgacgcc
    19561 gctgcggcgc agggcggcga ggaccagctt ctgggcgtcg gcgtccaggg tgctgacgat
    19621 ccgctcgccg ggctggtgca ggagctgcgc cgccgcgtgg ggcgccaggt cccagcgcgg
    19681 ctggagctgc tggcggtcca gggtgctctg ggcgaggcgc tgggccgagg cgcaggcggc
    19741 gggcgtgccg aggcgctcgg cgagcatgca ggcccggtgg ccgaccacct ccggccgcgc
    19801 gttggggccc cgcagcagca cggcgaggat cagggattcc ggccggttga gttcacccgg
    19861 cgtcttgccg aaaaaggcgc gggcggcggc atgcacgccc tgcagatcgc cgcggaaggg
    19921 cgccaggttg aggtaggcct cgaggatctg gtccttggtc cagcgcgcct ccagggcctg
    19981 ggcggcaaag gcctggtccc atttctggcc gaggctgcgg cgcggctggc catcgcgacc
    20041 gagggccagc tcggggtcga gcagcccggc gagctgcatg gacagggtgg aggcgccgcg
    20101 cttgcggctg ttccacaggt tatgccacag ggagccggcg aaggcctgcc agtccacgcc
    20161 agagtgctcg aagaagcgct tgtcctcggc ggccagcagg gcctccttga ggaccggcga
    20221 gagggctggc aggccgaccc attccaggcg acgcacctgc gggtcgaggc ggcgctcggc
    20281 cagcggcgtg ccgtcccgcg ccagaagcac ggccaccgag gggcgcgccg cggccttcac
    20341 gtcggcaaag gccggcaggg gctgatgcgc gcgggcaggc agcgccggca gggcgaggac
    20401 ggcagcaagc aggacggcgc cgatccgggt ttccggaccc gatgcccggc gcggacgcac
    20461 gccggcacgc gggccggaga ggcggcaggg acgcagaccg ggcagggacg ggacgcggag
    20521 caggcgcatg atcgggcggc attgtaggcc gcgggccggc gggcgcaggc gggttcaggt
    20581 cacgccggcg tcgtccgggc tgtcaattta tgtgtcattg gcggccccga ccggcacctc
    20641 cgtaccccgg cccagggcct tcactgcgtc gatccgcgcg gcatgaacgg cggcgggaat
    20701 gcgcgccttg tcggcctgct ggcgggcgat ggcgccggcg tccaccgcac gcaccgcggc
    20761 cagggccgcg aggagacggt cccgcgcggg atagggccgg ccggcgaggc ccgcacggcc
    20821 ggcgtggtcg caggcgcagg cgtcgagcag cgcgacgaag cgctcgggcc ggcgcaggcc
    20881 gtcgcagcgc tcgatgagct tgacggtggt gtccgggcgc agctcgaggg cgcgatggac
    20941 gatgccatgc tcgcgggccg tcatcagggc cagatcgcgg cagtcgacgg ggcacttcag
    21001 gcgttcgcag acggcgcggg cgaggcgctc gctggacgcc tcgtggccgt agtggtgggg
    21061 cagcacgggg gccggcgtga cgcccttgcc caggtcgtgg agcaggcagg cgaagcgcac
    21121 cggcaggggc tgaccggtgg cagcggcgtg gtcgaggacc aggcacaggt gctcgccggt
    21181 atccacttcc gggtgatgct ttgccggctg aggcacgccg aacaggcgat ccacctcggg
    21241 cagcaggcgc gccagggcgc cgcagtcgcg caggacccgg agcatccgcg aggggcgggc
    21301 ctccatcagg ccgcgggcga gctcctgcca cacccgctcc ggcaccaggg cgtccacctc
    21361 gccgtcgtcc accatgcggc gcatcaggac aagggtctcg ggcgccacgc ggaaatcggc
    21421 aaagcgggca gcaaagcggg cgaggcgcag gatgcgcacc gggtcttcgg cgaaggccgg
    21481 gctgacgtgg cggaacaccc gcgcggcgag gtcggcctga ccgccatagg ggtccatcag
    21541 ggtgccggct tcgtcgcggg ccatggcgtt gatggtcagg tcgcggcggg cgaggtcttc
    21601 ttccaggctc accgacgggt cggtgtggaa gacgaagccg gcgtagcccg gcgcggtctt
    21661 gcgctcggtg cgcgcgaggg cgtattcctc gtggctggcc gggtgcagga agaccgggaa
    21721 atccttgccc accggcctga agccgcgggc gaggagatcc tccggcgtgg cgccgaccac
    21781 cacccagtcg cggtcctgga ccggcagacc gaggagttcg tcgcggatcg cgccgccgac
    21841 gcaatagacc ttcatggccg gggtttccgg aaggcgtcga agtgcccgcg ccggttggcc
    21901 cggccgagcc accggggcac cacggtctcc agcgcggtgg gcgtgcggcc ccagggctgg
    21961 ggcgggccgc cggtgatgtt gtcggcgcgc atggagcgca cgttgtcgcg actcatcagc
    22021 gggccgggca ggcgctccag caccgccgcc tggagcaggg cgaggggttc cgacaggggc
    22081 acgatccgcg tgctgcggcc ggtcaggcga gccacatagg ccaccagctc ggccagggta
    22141 tacaccgtgg ggccggccag gtcgcaggtg aggccggcgg cgtccgggcg gaccagggcc
    22201 tcggcgacca cgtcggccac gtcctgcacg tacaccggct ggaagcgtgc accggcgccg
    22261 cccaggggca gcaccgggaa ggtccgcacc aggcccgcaa agaggttgag gaagctgtcg
    22321 ccgggcccga agatcaccga cgggcgcagg atcgtccacg ccagttcggg cgcgccggcg
    22381 cggatggctg cctcgcccgc cgccttggag cgctggtatt ccgacggccc gtccggcgcg
    22441 gcgccaaggg cgctcacgtg gacgaggcgc gtcacgccgg cctggcgcgc cgcggcgacg
    22501 aggagggtcg gcagctccac gtgggcgcgg gcgaagtccg gaccgtaggg ctgacccgga
    22561 cgggaatgga gcacgccgac caggctcacc actgcgtcca cgccctgcat caggcctgcg
    22621 agggtgggcg gatcgaagac gtcggcctcg accaggtcca catcgggcag ggtgaggagg
    22681 tgccggacgc gggcacggcg ccgggtggga atgcgcaggg acaggccgtc ccgtgccagt
    22741 cgcgccgcca cggccgtgcc gacgaagccg ctgccgccga cgatgagcac cttgcgggag
    22801 gtcatggggt catcctcgcg tgatcctggg attcgtcaga gtgccgcagc ggccaacaat
    22861 tcccggcccc tgcgccgacc gtcattcatt ctccagcccc gcgcccggcg ccgggctgcc
    22921 caggcccgag gcatcgccgt tgccgggcag gacggtgccg aggcgggcct tgagggacgg
    22981 cgcgcgaccg tccagcaggg cggcgtaaac gatggaattg gccatcacct tcttcacgta
    23041 gtcgcgggtc tcgttgaagg ggatggtctc ggcgtagatg gcgccttcca ggggcttgct
    23101 gtcgcgccac ttcctggccc ggccggggcc cgcgttatag gcggcggagg cgagcaccgg
    23161 atggttgtcg aggccttcca gcaccatgcg catgtaggtg gtgccgagca tcacgttggt
    23221 gtcgggatcc cgcagccagc caatgttgta gcccttcatg ccgagcttgc cggcgatcca
    23281 ctggccggtg gacggcatga tctgcatcag tccctgggcg cccacgccgg agcgggcggc
    23341 ggtcacgaag cggctttcct ggcgcatcag gccgtagacc caggcggtgt ccaggtcgcg
    23401 gctccgggcg ttgggttcga tgcgctcccg gtagggtgcg aggtagcgca ggctgtagtc
    23461 gtgttcgctg cgggtgcgat cggcggcgtt gatggcccgg tcgtagatgc ccagctcctg
    23521 ggcgtaggcg gcggcggcga ggagaaagcg gtcgtcccgg ttgcgcatcg accagttcca
    23581 ctcccgcgtg ccctcggtgc gcatgtcgag ccggaagagg gctacggcgc gctgcacgcc
    23641 gggggtggtg cgcaccaggg tcgcctcgtc gtcgctgggg ccgcgggctt ttttgggcag
    23701 tacaaagctg cgcccgagct cctcgtcggc gaggatgctg tagaaggcgg ggtggccgcc
    23761 gatctgctcg aaaagccggc gcgccgcgtc ctgtttgccg agggcggcct gggcacggcc
    23821 gagccagtag gcccagtcgg gctgttcccg caggctcgcg ggcatggcct cgatggcgct
    23881 gcgcacgccc ttccagtcgc cggcgcgcag ggccgcgcgg gcccgccagg cgatctgctc
    23941 gtcgctcatg ggtgtgtctc cggcggcgcg ataccaggtg tgggcctccg gcatcagacg
    24001 ctgggctgcc tgccaagcga tctggccgag ggcgtaaccc cgctcggcgg gggagaagcg
    24061 ctcgtccatg cgctgatagc gcacataggc gccgagggtg tcgtcgcggg cgaggcgggc
    24121 cagggcgatg agggccagtt cgcggccggg acgggtggcg gagaagttgg gcggcaggcg
    24181 gtccaggtag cccgccggat tggcggcggc cttgtccaga tcggacggtg ccggcacctg
    24241 gtcggcgggc agccagctgg cggcgtagcg ggcatgggtg ttgcgcttgg cttccatcag
    24301 gcggcgcacg cgggcccaga tctcgtccgg gctgaccaga gcctcgcgga ccagggtctg
    24361 gagcaccggc acacaggatt ccggcgtgtc cacgaggttg aaccacaggg gccgtgcctc
    24421 cgccagcgcc atgccgtcgc cgttggcgag gcgggcctgg aactggtagc actggagatc
    24481 ctgctccggc tgctccatga aggggtattc gatcgcgaag gtgccccact cgccgcgccg
    24541 ccccaggccg cgcagccatt cgccgcgcag cttctcggcg agccaggcgc cgcgattgcg
    24601 ttcgaggaat tcccgcaggg ccgccggatc gatgtcggcc gggcgcgcca cctgggcgga
    24661 gagccaccag tattcgggat agggatcgag gacgtggccg gccggcgcgg cgtaggccgc
    24721 cagacgcgcc cggtcgccgc tgcgggccgc gtcccgcgcg gccaggatac gttcgtcgcc
    24781 cgtatcggcc tgcgccgcca ggaccgccca caacactgcc gcgatggcgg cccgcacgct
    24841 attcttcatc gactgccaga ttgacctgaa atgcccgcgg agcatagcac agtgacccag
    24901 cccccctccc cgcccccggc gccagacggc accgccgagc gccgccgcgc cctgcgcagc
    24961 gcccgcatcg ccgcacgcca ggccctggcc ccggcccagc ggagcgccgc cacggcgcgc
    25021 atcgaagcgg ctctggacgg cctgctggcc gagctcgccc cgcgctgcct gggcttttgc
    25081 tggccttggc agggcgaggt ggacctgctg ccgtggatgg cccgctggct ggcggccgac
    25141 ccggcacgcc aggcggccct gccggtggtg gtggaggcgg ccgccgcgat gggcttccgg
    25201 cgctggacgc cggatacccc gatgacggcg gaccgccacg gcattcccgt gcccgccacg
    25261 gacgcgccgg tgcagccgga cgtgctgctg gtgcccctca acgtgttcga tggcgcgggt
    25321 ttccgcctgg gctacggcgg tggctacttc gaccgcaccc tggccgccct gcggccagcg
    25381 ccgacgggca tcggcgtagc cttcgagctg gcgcgggccg acaccgtgtg gccccagccc
    25441 cacgatctgc ccatggactg gatcgtcacc gaggccggcg ccttccgccc gcggcggggc
    25501 tgaaaggcgg ccgcgcggcg gggcgacgga tcaatgtccg accggccgca cgcccggcag
    25561 gtggctgccg aggatggcag cgcggatcac gtcgatggcg ccgctgcgcg gataggtgac
    25621 gcgccaggcg agggcgatgc gccgggacgg ttcgggatcg gcaaaggggc gcaccgccag
    25681 cagggtattg gtggccggga tgcggtcggc ggcggtgctg ggcagcaccg tgatgccggc
    25741 gccggtggcc accatgtggc ggatggtttc gagggaactg ccctccaggg tgcgctgcag
    25801 gccgtcgccg ttgcggcaca tggggcacac ctccagcacc tgatcgcgga agcagttgcc
    25861 ggcgccgagc agcagcagct gcgcctcggc gagctgctgg gcggcgatgg tctgctcggc
    25921 cgaccaggcg tggtcggcgg ggatcagcac gcggaagggt tcgtcgtaca ccgcctgggt
    25981 gacgatgccg tgctcctcga agggcagggc gatgacgatc acgtccagtt cgccgcgctt
    26041 gagggcgtcc accagcttgg cggtgtagtt ttcctggatc agcaggggca tctgcggcgc
    26101 ggccgcatgg acccgcggga tgaggtcggg cagcaggtag ggccccaccg tgtagatgac
    26161 gcccagacgc agcggaccgg ccaggggatt gcggccggcg gtggcgatct ccttgacctg
    26221 cccggcctcc agcagcacgc gctcggcctg ggcgacgatg cgggcgccga tgtcggtgat
    26281 cttgacgtcg gccgcgctgc gttcgaacag cgtgacggcc agctcgtcct cgagcttctt
    26341 gatggccacc gagagggtcg gctggctcac gtgacagcgt tcggccgcgt gcccgaagtg
    26401 acgctcgcgg gccagggcaa cgaggtagcg cagatcggtc agcgtcatgg gggcggctcc
    26461 gcaacgacga taggtttcaa ctatacaacg ccgcggccga aaggttcacc cgtacgcgga
    26521 tttccgcgac acttgcgcga cccgtgggcg ccgtgcatga caaggagatc gcccgcgccg
    26581 caccggcccg gggcctttcc agagaggaag cggccgcccc gccggagccc gggcgcgtgc
    26641 ccgcatcgcc cttgatttgc agcgaaccgc cctcaatttg taagggcgtg tatgtatcgg
    26701 gaacgcccgg ccccgcgcgg gcgtccatgc agcgttcgaa cccaggagtc tgtgcatgaa
    26761 atctgccgtg ctgcgttaca gccttgccgc ctcggccatc gccgtcgccc tcgccgggtg
    26821 tctgccggag ggcgccgcca tttcctcgtc ccacgccgcc aatgccacat cggccacggc
    26881 gccggcggtg cccgccgctg cgaccacgac cgccggccgc tacgggctgc cggatttctc
    26941 ggcgctggtg gagcaggtcg gcccggcggt ggtgaacatc agcgtcaccc agaaacgcac
    27001 cctgagcaat ggcgacgatt tttccggcga ccccttcgac gagctcctgc gccgcttcgg
    27061 gattcccggc atgcccggaa tgcccggcgg caacggcggc gggcgggcgc cccagcagat
    27121 ccagcagggc gtcggatccg gcttcatcgt gagcggcgac ggctacgtgc tgaccaacgc
    27181 ccacgtggtg gacggcgcca ccgaggtgac cgtgcgcctc accgacaagc gcgagttcaa
    27241 ggccaaggtg gtgggtgtgg acaaacgcac cgatgtggcc ctcatcaaga tcgacgccag
    27301 cggcctgccc acggtcaaga ccggcgacgc cgagcacgcc cgcgtgggcg agtgggtggt
    27361 ggcggtgggc tcgcccttcg gcttcgagaa tacggtgacc gccggcatca tcagcgccaa
    27421 ggcgcgccgc ctgccggacg agaactacgt gcccttcatc cagaccgacg tggccatcaa
    27481 cccgggcaat tccggcgggc ccctgttcaa cctcgccggc gaggtgatcg gcatcaactc
    27541 ccagatctac tcccgctccg gcggcttcat gggcatctcc ttcgcgatcc ccatcgacgt
    27601 ggcgatgaag gtcaaggacc agctccagcg ctacggcaag gtctcccgcg ggcgcctggg
    27661 cgtgtccatc cagggcctgg acccggatct ggcccagaac ttcggcctcg acaagcccgc
    27721 cggcgccctg atcgccaacg tggaaagcgg ctcgccggcc gaaaaggcgg gcctgcagag
    27781 cggcgacgtg gtgctcggcg tcaatggcca gaaggtggag aactccgccg acctgccgcg
    27841 catcatcggc gagcagaaac ccggcaccag cgtacgcctg tccatctggc gcgaccgcaa
    27901 ggcccgcgag gtgagcgtga cgctgggtga acagctcgcc gagggcgccc agcccctggg
    27961 cggcttcgag cgcaagaacg agggcgccag cggcaagctg ggcctcaccg ggcgctccct
    28021 cacggccgcc gaagcgagcc gcctgggggt ggccggcggc ctgctggtgg aaggcgtcaa
    28081 cggccccgcc gccaaggccg gcatgcagcc cggtgacatc atcctggggc tcaacaacca
    28141 gccggtgacc agcgtcgacc agttccgcaa gctgctggaa ggcgccggca accgtttcgc
    28201 gctgctggtg cagcggggca acagccggat cttcctgccc atccgcatgg gatgaggcgg
    28261 agaccgttcc gcaggccggc aaagtttgcc aagttgtcat gacaacggaa caagccgaag
    28321 cgggctggat ataatcggag cccgccggat gacagagggc gcatccggca cactccgata
    28381 aaaagctccg taatgtcatt gttccggcca ccggccggcg tcctgctggc gctggccggg
    28441 ctcgtttctg gcgccgccgt gcacgcgtcc cccgaagaat cgccgttctt cgaggacatg
    28501 ccgaccgtgc tgtcggcctc gcgcctgcca gaaccccttc acgaggcgcc cggcgccgtc
    28561 accgtcctcg accgggactt catccgcgcc accggctacc gggacgtggc ccgcctgctg
    28621 cgcctggtgc ccggcatgca ggtcgggcag gagcgcggcc acgcccagtg ggtcacctac
    28681 cacggcctgg gcagcgatta tccgaccgag atccaggtgc tcatcgacgg ccgctcggtg
    28741 ttcgccccca gctccttcgg cggcgtgaac tggtccggcc tgcccctcac cgtcgaggag
    28801 atcgagcgca tcgaggtggt ccgtggcacc aactccaacg cctatggggc caacgccttc
    28861 ctcggcgtgg tgaacatcat cacccgccac agccgccagg atccgggcgg acacgtcgag
    28921 gcgaacgccg gcacgcaccg catcggcgac ctggccgccg cgtgggccgg cggcgacgag
    28981 cgcatcggcg tacgcctgtc tgccgccgcc ctgaacgacg acggcttcgg cagccttttc
    29041 gacggacgcc acacgggcct cgtcagcctg cgcagcgacc tgcgcgcgag cacgcgcgac
    29101 gaactcaccc tgcgggccgg attcgccgac cagcgccgcg acctgggcta cccggactcc
    29161 gtcttcggca ataacgccca gcgcaccagc accacccaca acggcaccct ccacctgatc
    29221 tggcgccata ccgacggccc cgacgaggag tggatggcca gcctctaccg caaccacgag
    29281 tcgggcgtgg accgctggaa cgccagcgca ccgcccgcct accccagcgt gcccctggac
    29341 cgcaacgtga gcagcatgcg caccaacgcg gaattccagc accgctttgc cctcggcccg
    29401 tccacacggc tggtgtgggg cctggaaggc cggcgtgacg agaccgacgc ggacacgctc
    29461 ttcgccggcc tgcgcccgcc gggcttcgag ctgtaccggg ccttttccaa cctggcctgg
    29521 catgccacgc cggcctggca gatcaacctg ggcgcgttgc tcgaaaagag ccccgacacc
    29581 gcggcccagt tcatcccgcg ggccttcgtc aactggcagg cgagcgccac cgacaccttc
    29641 cgcgccggct acgcccgcgc ctgggagcag cgcaacctgt tcgaccgcta cggcgacgtg
    29701 cgcgcctacg atccggtcag ccgcgccctg ctggcgtggc cctacatgcc caatcccgcc
    29761 gcccgcgtgc ccaaggtgga caccctggag ctgggctatc tgggccgctt ccgcgccctc
    29821 gacgccacgg tggacgtgcg cctgttcaac gagcgcatca ccgacttcgt catccgcgag
    29881 gtgatcgatc cccccatgcc ggtcgcgctg cccctgcgca cctcgcgctt cgtcaacctc
    29941 cccacgcccg tggtgctgcg cggcgtggaa acccagtggc gcctgcgccc ggcgcgggac
    30001 accgaactgc tgatcaatca caccctcatc gaccgccgcg ccgacgacgc cacggtcacc
    30061 aaacgcaccg ccccctatgt agccagcctc acctggctgc agcagtacgg tggcggctgg
    30121 tcaagcacgg ccagcctgct gcgcatgggg ccgctggccg gcggctatgg cttcgtgccc
    30181 ggctgcgact acgccgcgcg ggcctacacc accgtcgacc tgcgcctggc ccgccgcttc
    30241 acccggggcg gccggcagat cgaggtcgcc ctcaacggca tcaacctggg cgcgccccac
    30301 caggagatcg ccgaccgctc cgagcagtgc ctgcccgccc acctgggcac gccggtcaat
    30361 ccggcctcgc cgatgggctg gctgtcgctg gcggtggcgc tgtaagcccc cctcacgtcc
    30421 cctccggcgg accgacggtc ttgcggatga agaccgcgtg ctcgattgac gtcacccaca
    30481 ccaggccgtc gccggtctgg ccggtgctgg ccacctgcac gatggcctcc accagcgcat
    30541 ccaccagatc gtcgggggcg acgatctcca cccgcacctt ttcggtgaag tcggtcagct
    30601 cctcgcggat ggaggcgggc acatgccgca ccgtcgcccc gcagcccgcc gccttgctca
    30661 gggtcatgcc gggaaagccc ggcacggccc gcagcacctc gcgcaggcgg ggcagcttct
    30721 gcgggcggat cacagcacgt atttccttca tgtcacatcc tcaggaagcc ccgcggcggc
    30781 gcagccacag cccgccgccg cggcaacatc acgcctcgta gggcgtatcg tcaaaccatt
    30841 tgtagagggt cggcagcacc accagggtga gcagggtcga ggtgatcagc ccgccgatca
    30901 cgacgatggc cagcggacgc tgcacctcgg cgcccggccc gctggcaaac aggaagggca
    30961 ccagcgccag cagagcgacc gtggcggtca tcatcaccgg gcggaagcgc atcaccgccc
    31021 cgtgcaccac cgcctcggcg ggcggcatgc cgtcggcgcg caggtggcgg atgtagctca
    31081 ccagcaccac gccgttgagc accgcgatcc cccacagggc gatgaagccc accgacgccg
    31141 gcaccgacag gtattccccc gtcaccagca ggccgatcac cccgccgaca ctggcaaagg
    31201 gcaggaccag gatgatcagg ctggccagct tgagtgaccc gaagagcagg aacagcagga
    31261 agaagatcgc gccgaccgtc accgggatga tgatggtcag gtgccccatg gcccgctcca
    31321 ggttctggaa ctggccgccc cactccaggt aatagccgtc cggcagctgc acctgggcgc
    31381 ccaccgtctt ctgcagttcg gccacgaagc cgcccaggtc ccggtccttc acgttgatcg
    31441 ccaccaccac ccggcgtttg gccagctccc gggagatctg ggcgggaccg tcggcgatgc
    31501 ggatcgtggc cagattctcg agggccacct gcacgccccc cggcgcgctg acgaggatct
    31561 tgccgatctt ctcggcgttg tcgcggaagg cctcgggcag gcgcaccacg gccgcaaagc
    31621 gccgctcgcc ctcgtagatc tcggtggcct gacgcccgcc gatggacgcc tcgatcacgt
    31681 cgttcacgtc ggacacgttg aggccgtggc gcgcgatggc ggcccggtcg atctcgatgg
    31741 acaggtactg ctgcccgctc acccgctcca cccggatgtc cgtggcgccg gtgatgccac
    31801 ccgccacccg ggcgatctcc tcggccttga ggcgcaggga ctccaggtcg tcgccaaaga
    31861 ccttgatggc cacatcggag cgcaccccgg tcaccatctc gtccacccgg tcggagatgg
    31921 gctgggccat caccacctgg acgccgggca gttcctggag ggcatcgcgg atggcgtcgg
    31981 cgatgtcgtc ctgggtccat ccggcgggcc actcgtcgcg gggcttgagg ctcacgatgg
    32041 gggtcgactc gttctgcccc tggggatccg ccggcgactc gccacggccc acgccggaaa
    32101 tcgccgagcg cacgccgggc acttccatga cccggcgcat ggcctgcatc tccatcttga
    32161 ccgactcatc cagggagatg ttgggcaccc ggttgatgcc cggcaccagc gagccctcct
    32221 tcatctccgg gatgaaggcg gtgcccagca gggggaacat ggccagcgtg gcgaccagca
    32281 gcaccacgcc gcccagcgcc gtcgcgcggc tgtgggccac cgcccagtgc agcatcttca
    32341 ggtagggcgc cttgagccgg gcgatgaccc aggtatcgtg ctcggcaccg cccttgagga
    32401 ggtaggacga cagcaccggc gtgagcgtca gggacagcac cagcgacacc gccagggcaa
    32461 tggcgatggt gtaggcgagg ggcgcgaaca tcttgccctc catgccctgc agggtcatga
    32521 ggggcaggaa gaccaggatg atgatgccca caccgaagat caccggcgtg gccacctcct
    32581 tgaccgcgtc gaagatgatc cgcgcccggc tctcgccact gcgggcatgg cccaggcgcg
    32641 cgaaggcgtt ctccaccacc accaccgagc catccaccat caggccgatg gcgatggcca
    32701 gcccgcccag ggacatgaga ttggccgaga ggtggtagcg gttcatcacc atgaaggtga
    32761 cgaggggcgt gagcaccagc gtggccacca cgatgaggct ggagcgcaca tcgccgagga
    32821 acaggaacag cacgatcacc accagcacga tgccttcgat gagcaccttg ctcacggtcc
    32881 acagggccgc gtccaccaga tcggagcggt cgtagtaggg cacgatgcgg agcccgtccg
    32941 gcagcatgcc cttggcgttg atctcggcca cccgcgcctt gatgcggctc accacctcct
    33001 tggcgttgcc gccgcgcagc atcatcacca cgccgcccac ggcctcggtg ctgccgttct
    33061 tcaccacggc gccctggcgc acctcgtggc cgaagctcac ctccgccacg tcgcgcacga
    33121 acaccggcgt cccgccgatc tccttgagca cgatggcgcc gatgtccgac aggctcttca
    33181 ccaggccgac gccgcggatc aggtactgct cggcgtactg gggcagcacg ccgccgccgc
    33241 tgttggcgtt gttgcgcgcc accgcctcgt acacgtcgcg caccgtcagc tggtagtgac
    33301 gcaggcggtc cgggttcacc agca
//
LOCUS       DAOQPA010000028        32894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_594_length_32894_cov_17.775389, whole genome shotgun sequence.
ACCESSION   DAOQPA010000028 DAOQPA010000000
VERSION     DAOQPA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 32894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32894
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_32894_cov_17.775389"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1985)
                     /locus_tag="PKX01_05960"
     CDS             complement(18..1985)
                     /locus_tag="PKX01_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ75704.1"
                     /translation="MNAPLASARALDGAVLNRAFVRAGLARFGLTRLAAPSGLFAELA
                     ADAGLHCLLDSSPDDWAAACAALAARAPSLGGAWGALLTDYRLQLPEWFALALAAEVE
                     ADHAVGRALAELQAPDADGRPALHLLDALAATLFDAALPPLAWPHHALVQRGLASLSG
                     DLPLVRTSLRVDPRLAALLAGDRRPWADFTPLPPDTLTHVLPATLLTELDAAAHLVQQ
                     GAARLVVLRGSHGTGRAAAARLGAALGLLPVTIPPDPWRSTPIFAAACRYGGWLPVFL
                     TDLGPGDTFSLPTGSLEQTPQVLVTGQDGSVDTPDAIELDIPPLERHERHRAWQAVLG
                     PDAPPVLADHARLDGPAIHALAQAVGRDAARRHEMPALAHLRRARSTHGAERLRQLAQ
                     PVHRDVGPDGLVLPAETARQFEHLVRRCRMRERLWDGLGPSLASPAPGVRALFAGESG
                     AGKTLAASRLATELGAPLYRVDLAAILNKYVGETEKNLGRLLDEAATLDALLLMDEAD
                     ALFGRRSEGKDGGERYANMMTNFLLTRIESHPGIVILTTNARNRLDAAFTRRFDTIIE
                     FPLPGVAERLRLWHAHLGARSPGEAACGLLATYSDLPGGHIRNAVLYAVALSPRPPGT
                     PVEITLLVAGLAEEYRKLGRTPPPQLAQIGE"
     gene            complement(1982..3625)
                     /locus_tag="PKX01_05965"
     CDS             complement(1982..3625)
                     /locus_tag="PKX01_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75705.1"
                     /translation="MDRASATVTIARLNLRGARDDVARARTPLLAALDRAPWPDPGPD
                     TLLFVRHLRLRGSATDIARRAADLVRELTPRAEDGWSRAADQADAVRFRSRADMSACL
                     LADLLDGRAADRWFWARRAELFERPVPDAIVRILEEAPFELPELLDAGSARARPLAAI
                     ELLPRLMARLDAPATERLLGALRAATGWSLRFAAPEATAMAPAPGLPTALLATATRQA
                     HALRLAPAAPAARLVAVLLAWRHVPAHLGTPDADVLLTRLTHLVTHDGARSAPQQPMP
                     IARDATVPAPADTPSPHPAPLPTAAPVEARTTGAHPGRTTTTSDASREPASDAPAAEC
                     AAAALHSPRAAAPEDGFVTSCGGLLFLLNVLALTPVQSLLGDRNLPGCGWRWLARLAA
                     TLGLEPDPPLAAFLATEAALASPDALAVLPPLAEEARLEAFAAARYGADLWRADAFRL
                     PARIVATPSHVDAHFRLADARLDLRRAGLDLNPGWLPWLGRVVTFHFNDGPRALPSPM
                     GKPEDFTMGRDHSPALSPAGPPDTGPAAPAAAKKPGERP"
     gene            complement(3641..5539)
                     /locus_tag="PKX01_05970"
     CDS             complement(3641..5539)
                     /locus_tag="PKX01_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75706.1"
                     /translation="MNLLNKSGVFDNIADLKLQVGVKPSAALPQVEKLSTQQAFLTLE
                     PQTQAIAAFAVAAQAVESSAASAAARPADPIAIGTIAQTTQSLGTIRQFDVVQNYYST
                     ALENLTALRRIAIALGEDGKPTLDTTTTLLARVRRFLIDQLGSEDALNARLGQHGIAA
                     FSNLFTTLPPKIGATFTDADSKSATHNAHSALFHHGAVITDDIRGIEALRAVLVALRQ
                     DHQNQLAALQDQLATVETQLPEAAARLDTLNHTRLEALDDYAAAQHTLAEHWDAVAQA
                     YAERRRVIDSHRGLFYVKQRETPVTVTLPDPQDLRFTSPDDIVPGVPDREIPLADDLA
                     PFMEVVLDIPVADWAVLSGLAHLLPSRPRLELMVATRLQKVALRQKIGLPVATARLQG
                     LAFQNRGLLDDIALRPFAASAMLDLQRQGHAILALDDLLASPVPLLREPARVLHRRLD
                     AAAGGLLDLLRPIAPSLRLAWAAAAEADTLPVERPERWPGIAQAEAADLNGLRTFGEL
                     VAWWFRQLDSDASAASRTAMRNFVRACVMLATGDNPDEILQGHVSSPPRRFRIGEVMR
                     LELNREPRPGALLQLLDDAQRLVGTLRVDDHDNGQTVASIVRVLDDSLGVNRPLTTAF
                     RVTGSRNP"
     gene            complement(5555..7243)
                     /locus_tag="PKX01_05975"
     CDS             complement(5555..7243)
                     /locus_tag="PKX01_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75707.1"
                     /translation="MTASIHVESPQAGPDDPRRLNLFPGLALPEQAFRKLQDYTDDRM
                     APLAGRLQPGILAGFEVSLEEAARAPRLRFRPGRGVTGAGQLVRLYTPLVTAWSDLLT
                     LAERDRGNAPLKDGLYLLTLRSVVEEVDALDTRSPAIRTEADPTRERRLEVVTLPGLV
                     ELSRSPRLLSLPQQETVNRLSVGFLDASPFTLAVGAVPVGMVKVADRQLAWLESVGGR
                     FMAEADAPHRTFLAHTLSVLQAGLQPLGSDASPAGFLLTERLGLSYLPAAGALPPALL
                     RNPADHPPQLDFNPSDLGIDLIPVPANTVEGIIAEELSRGYVDLLHPRRDRLRLMLAI
                     PDLDYRPDLLDLPLRDVRAEATLCERFDTAEAARQAWRNQWQKVFGNLAPVELLDTLL
                     QGTQPRLPGDVADQEPVAAELARPERSATRARVEEVRPPVSPDAFRGARRSLLVDAFS
                     AGKMADDPVFWLDDHPPRYEAVPAALPETDTTDGLLRQRADLRAQIKDMQTRLETSYK
                     LLNELNDYLGLQRQHFDALTVSFSALAGGVAGDGSGLKVMRWTNAASYTPASTK"
     gene            complement(7271..9883)
                     /locus_tag="PKX01_05980"
     CDS             complement(7271..9883)
                     /locus_tag="PKX01_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HMZ75708.1"
                     /translation="MANTLQFQPYAPGTTATAGGARLGSIDPRLSRTNYFDGQLLKAT
                     DLTRDQIYLDERLLELGQAFGAGIAEGLDLDIADGRYVRVQPGLAIAPSGRVLKLSGR
                     VLEADLQNSALIASLNNGAYRQFPRGLYAVVLQHAEVASGVAEAYPTDLESRRQWKVS
                     EYAEGVELVLVPLPIGLPRGDALSLRATLAREILHASGSLMLPSDDAIALGLVAIEQA
                     VVAWYDKGLVRRRLRNPLAPAAQGEDLALHYVELLHDVLDARSHAGLGDGFPAAQYFR
                     MLPAHGPLPKAAIDPVNGTQIFFPKAYEVSIAPVRRSDLPRLLADSALLAPIDLERDA
                     DIDVMVLVPMDDASFALRARQLEADPVTGTLGKTGILAHLDVFALRLYAPPALGTEDR
                     RTWEQIWAAVAPSDVRYVRRPPRTAETNVSAVVLARGFAIPEEITLPQRDLLDSEHQL
                     DQALTDIQALQVELSQQRPHIQDLEAQLATASGDLTTARNQVDSLNATNTSLTDANAR
                     LTGDLANLKTTNDTLTGDLQSATSRADEADARATALQGTVAELNTTITQLQARIDENP
                     DQRVAQLTTDLAAAQAAADTARQQLADTTSQLNAQRTAAEAATNQATAAQARADDLQN
                     QLNSANGRVASTESDLAGARDALNASNARLSATSAALDAANNRIGGLQNDVTTAQNAL
                     TVARGDADQLRGQVAGLNVELSNQAQIQAGLRNQIAADQATQAQLTAQINQQASQINA
                     LNNQIQLLQRQLADLRANPNASGVTLADVARVRGAQMDLGLKLDDQLRGRPSEVLNAA
                     YDLFLLIGVEYDEVLWPSLQDLARQPDRLPKLRDELVQLLTVQRMTVPKAMVQAGQNA
                     GLSPNLINQWANQR"
     gene            complement(9891..10826)
                     /locus_tag="PKX01_05985"
     CDS             complement(9891..10826)
                     /locus_tag="PKX01_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75709.1"
                     /translation="MSQSDDNAATDSSLPDDGVFSEPLRRVSFEPGMLLGIEATRAEQ
                     AYHRRRHTRHAYWLHGAGTVAGLRVVMDSTPPKTAGKPWRVRLIITPGIGVDGLGREV
                     SVHEPYAIDLGDWLTAQHADSTAWGALERDGLDTAGTTLHLRITMRYQDVPSGLQPVM
                     ATAINAGTDPVGPSRIQDTVLYELTPWRPAIGDVPERPFTAHDDLSAYDDIADRLADH
                     ERAAIAAADAAGRRQLQLGARLLFALSDDNTALATRESLAVTAAELARTLLATVTIAL
                     DPGGQLVIDPARIVVDNLARPFLFNASTLARLLRQ"
     gene            complement(10823..13219)
                     /locus_tag="PKX01_05990"
     CDS             complement(10823..13219)
                     /locus_tag="PKX01_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75710.1"
                     /translation="MDSNGTRFLLLDRAADFRNAGGSCAWDADARAFTLVRHDEPRLP
                     RLAAADALQAWADAGPWILDDYGQIARLSADRRSIEAAVGWPDAPWNPLRAHRDPTAP
                     LAADLEALTLDPVDAPTACAFTDLHLGGSGLLAASWMADDGTRAGVTLVHLRARWQRH
                     LEIASPEAPAFRPVRLWVDASDRVWVASDTHLTLTIGAPLDPPYVPRPDRFEPVSVEP
                     SPPRVRWAQALPPLRRINAIAVVATRLLILAEASGSTDGAPQQILLVRALSDDPTAAL
                     TALPLPTGLPFASDIAALDDGRAILLPPLETGVTRAQRRDCPVITLDDPPTLAAERWP
                     RRSEAAVRFVRHRDNQVRHLANDGVVRLFRLAQARFLPHGRALLTPVFDAGTPDTVWD
                     RLILDACIPAGCSVMVEAQAADTPGGFADTFHRQPLPLALTTPDIPFATPRGTLHEIL
                     LQRSSGAARELRGRYLRLRLTLGGDGRHSPAVYALRIWYPRFSWQAHYLPDHFHQQQP
                     ADPEPVPAGRAANGADLRERLFASFSGLLTPIEDRIAASEQLLDPLAVPAPWLANLAA
                     MLGTRLRPHWPETRQRRWLAALGRLQPHKGSYGALCQALDILTEGGVSRGEVVPVELF
                     RLRRTLATVLGLSMDDARHPLTLGTGQSGNSLVGDTLVLSEDHAVEILALFAPELARP
                     GTERQAVEHFFDRYARRLTVILHGPAQSLRGVIEEALPELVPAIVQWNVLVSEHPFVL
                     GLSPLLRIDTYLETQPPPKPVRLDTTRLGRGDLIYNPVALLPEQAGSLPPPESEGDRA
                     "
     gene            complement(13223..15400)
                     /locus_tag="PKX01_05995"
     CDS             complement(13223..15400)
                     /locus_tag="PKX01_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HMZ75711.1"
                     /translation="MPLPLPDLDDRRFDDLVAELQQRLQRQVPELTALAPGDPAFALV
                     DLFAWLTETILYRANRIPDRQRLAFLNLLALPLRPARPARGVLCIDPSGGDGRSLPPL
                     VRTETALAAGQIALTTVGELQPLPLELHVLVKRTLDAATLSAEGISLAQLRQQYGVEP
                     AAFRPVRLTPPADTVTTAGSLDDSLYLAFAVPKALVRKADAVRRLLAGVVLNLALAPA
                     DDLDGELAASLSPRRLDWDVAVWRDPANHPESVVWLPLETVADSSRGGRQAGVVRLRL
                     PRDGSFLVVPSAIDPQFAGMGDTPPEAPADLAAGQLQCWLRVRSADGDGIELGYLGVN
                     GVDVLAQGVARDVMLGIGTGRPEQSLALPDAAVDTSDVRIDVESAGRFEPWQQVDHFG
                     AAGPDDAVYLVDGDSGSVRFGDGLRGRRPSDGLRIRASYYRFGGGTAGNLAPGAVQSV
                     AVGGGRLKPRQEWPLRGGVDAETVAEAEQRIPAFLAHRERAVTAEDFAVLARDNPLRP
                     IARADAAAGFLPGASLTAVRRNVPGVISVFVLPPAEPAFAAFPRPTAGTLRDVFEYLS
                     PRTLLGTELYVLSLQYQPVSVAVSVEVVDPALEQQTFRAVEQALLAYLWPLPPHGVRG
                     AGWPRGRAVDINELRTQASRVDGIEAVNGLRLFYQDLASGLWRELTGRLSLPLTDYQL
                     PELMAVAVQSGEATPAPPRAFAPGTSTGGSTRAVPVPVIPDVC"
     gene            complement(15411..15803)
                     /locus_tag="PKX01_06000"
     CDS             complement(15411..15803)
                     /locus_tag="PKX01_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HMZ75712.1"
                     /translation="MASSAPRLLSFPLLDGVAPDGRLAWADGDKSIREVMLNILLTRP
                     GERLLRPDFGAGLRDFIHHPNNETTRALVADAVQRALVRWEPRVSIEEVRVVPDPQRL
                     SHINLSIRYRLRQDGRRDSLDLAIELGA"
     gene            complement(15807..17429)
                     /locus_tag="PKX01_06005"
     CDS             complement(15807..17429)
                     /locus_tag="PKX01_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75713.1"
                     /translation="MVNVPVAARPPSVHHTPRLLPTWFAGGADEGMSLHRYLDDQFVP
                     RFLQDAQAGRLTATRSQGWYQSDRVGRYKDAPLLRLPMHRAFYIACCEVSCDTFGLPA
                     FDPVKVRSAGFVVRRGLSGGAVQRWMIREGEALGWQGGAGDGGEPDEYRRLLHHGLLK
                     PRFPEPPYSGEETHPLHPALVKARDASGHVRNRTLLWGYLPLGGTYREQGGVEIPTDT
                     DTALGQEIAWPCGSRGARAWQAQDSRPVQLGYASQGFRELLSVLLRYRITDATQPDNE
                     ALRALLGQIHFYPAPSDRFAVPAPSTHGTSLLEWLDRSEAAVLDWLTRLAAGSATLTS
                     APLPSQSGLDAAGKPLSVPVVLDVYLSQTQAALLRQLVPARGRRAMARNDDGIAFPRY
                     PQGEDDLFHAVPFVRWEDDCGCERTVWGRPSLPFRVASPLDPDFQRPQAILLPRLDDL
                     KRGAARGIALMAPKSLADKLLGIAPKMPPAEGGPGNACGLCWVFSLSIPIVTICAMII
                     LMILINLLNLIFGWLPWALLALPRLCGKLLKD"
     gene            complement(17423..18061)
                     /locus_tag="PKX01_06010"
     CDS             complement(17423..18061)
                     /locus_tag="PKX01_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HMZ75714.1"
                     /translation="MSPHSLGPLHELHLASVLDNSDPDNRGRLRIRLAATGLETWAGV
                     IVASAGNGYGVSLLPRVGEQVVIAFVSPDLPLVIGALWAGGSSQPGDAAPVDQRYLVQ
                     TPAGFKLLMDDQAQRVRIETPAGYHFTVTDQGSGTVTIEKGAEKIEMASGGITLTTGS
                     KVTVQASSVEVSAGSVQVNAGMSKFSGVVQCDTLISNSVVSSSYTPGAGNIW"
     gene            complement(18071..19117)
                     /locus_tag="PKX01_06015"
     CDS             complement(18071..19117)
                     /locus_tag="PKX01_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HMZ75715.1"
                     /translation="MPSIDAPLLNVRPRFRVDGQDRPNLGEAVLALDLRLPRSGMAGA
                     EVRVLNWSGSGSAATFVFNDIRLGQRLELFLGEGANATAAFSGDITALEERYGDGAPQ
                     LVLLAEDGLHRLARRRTSRAFEDQSLDDVIRRIASDAGLQADVQAGSDQATWLQNNES
                     DLAFLLRQLEPRDIGLRLQDGRLRARDDEADPRPARLTVGANAEKIRLIADLNHQVSR
                     VQARGYDLAAATDLSGDASSLVPAPAGVSGVATLGSLGWNTVSTRPHPAPRTAGEAND
                     LARKALRQSARRFVRGEIVCRDTPDLRGGREVELVDASPRLAGRYRVMDCHHRFDASA
                     GLRTHLRVERPDLS"
     gene            complement(19104..19253)
                     /locus_tag="PKX01_06020"
     CDS             complement(19104..19253)
                     /locus_tag="PKX01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75716.1"
                     /translation="MPVVIEELSAEITPDPHESPAQPAEDAAERAVLDLIELAREREA
                     RLAVD"
     gene            complement(19262..19960)
                     /locus_tag="PKX01_06025"
     CDS             complement(19262..19960)
                     /locus_tag="PKX01_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75717.1"
                     /translation="MSPAGAPPSFAKAVLIPMNGDQVETDPAKHIPVQFNPSTLRVTL
                     ANTLHADSGGGRRGRRPAAQYVEKSESSLAVELVFDTSVAQQGTPANSDVRALTGRIA
                     ETFMKPVDPDSARPGAPKRCRFQWGSFKFTGMLASYGETLDFFAPEGIPLRATLALTF
                     KEDRYQFDRDESVRAAARAQPAFAPGGSDVSADRATQAAGGDPRDWRGVADLNALENP
                     RFTPAGGVRIPGRD"
     gene            complement(19957..20448)
                     /locus_tag="PKX01_06030"
     CDS             complement(19957..20448)
                     /locus_tag="PKX01_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HMZ75718.1"
                     /translation="MADSAPVPAEFIPFRFRVALFADEGGSQLVCRGAFSEVSGLEAS
                     VAVKNLKEGGRNWGEVQLSGPTTFSPVVLKRGFTEVGDLWKWFDAMNRQANYALRYTG
                     LIEVFHPSRAFTDGPTVKWWLHQVLPVKFKGPDLSATASQVAIEELHLAHEGFTLEAG
                     GAP"
     gene            complement(20441..22063)
                     /locus_tag="PKX01_06035"
     CDS             complement(20441..22063)
                     /locus_tag="PKX01_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75719.1"
                     /translation="MFLADQRIEIDARPAPPVPPVLEVVMLGHCPGPDDGSGASGAQW
                     QRVADDEALLADIAALGFSREVRRLKALRAGVDSAGRSVTPEPGDADLRALYRLPLPI
                     HALGQFRVLFPYSFDTPCRAVSTLAGDRAWLPRAVQDFFEDSTSLGREALKLWVIRVP
                     EAEGAHGFLPDTGAQLTDIGRLGAFERALLPPRAGLIVLPDLERLQVPALLPDIPRMQ
                     LDNPVPAFLPVGTAVDDGHRERRRGDEIPHATEPLPPEAVIEPLIRTLARLRPDMQCL
                     LALPLDADAGQEVPVPAPAWTAYLTRTVGSALKEGAASLDAASALRHLQWIYPYLRGA
                     DRPLGSPCGLVAGLQALTAQRAGPWRSIGERPFAGRDLPWPVLDQNQAAALRQAPGVT
                     VLLRRNGRIVVDDERLCVPCLPLPALRQLDAARRGQDHWYSAEVMRFMGWLQRQLRAL
                     GERLVFDADVHDPRTEMALRAFFTHLHARGALRGARPEDAYRLAIRSDGDSTLIADIE
                     IAPAFPLDLIRITFLQDRHNADARVQMEALDG"
     gene            complement(22071..22529)
                     /locus_tag="PKX01_06040"
     CDS             complement(22071..22529)
                     /locus_tag="PKX01_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HMZ75720.1"
                     /translation="MAQMRETPYAAFNFLVELQPGQGKEVQAGFSEVSGLNAEVTVAE
                     YRAGNSTVNYVHKLPGIHKAGDVTLKRGVIGAQNLYEWLEASRSGRIKDAKREIVIKL
                     LSEDRADTVVSWKLRGAMPIKWTGPTLTAKGGGDVAIEELVLSVETVEQE"
     gene            complement(22546..24714)
                     /locus_tag="PKX01_06045"
     CDS             complement(22546..24714)
                     /locus_tag="PKX01_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HMZ75721.1"
                     /translation="MPEYLAPGVFIEETSFRQKSIEGVGTSVAALVGPTRSGPLRGTP
                     EALTSYAEFERTFGDAEDLKLGGSATLNLTSHAARAFFDNGGKQLFVVRVAAGGNDSD
                     ATSTGSSATAASVADSKGNLAFKSRFPGAQGNLTLEILWRDTENLLASETVASAADGV
                     QYYLDARGVVEAAKVTGAITSDKFPVDIQAIVQKDGANLRIPDARATLVSKADPANPA
                     PVGANQITVLDPTKLPAGTQLRRVYARQPSAGALSADALAVLILKAEADLSAFTKGDH
                     WGKALVLRGTLDKGGQTFTVKGDATLNPGITKDVSVPLAALASVPGAAGALVVQRSFD
                     LEVRNGGPDGEILYVYGSLDFTASGDRSLAKALPAAPERKEDALHSPVACTVKTLDGA
                     ALAATLYTLFTPAALFPPAGSYKDPRYLITLSGGTDGNVPGAGDYAGETNELLGSTGL
                     AALEAVEDVSIVMVPAAAAHADTHTAVLMEMLKHCRRMRYRVGLVDSREGMSLGEVRT
                     FRNDFDDSRLALYHPWVTIADPTGQRREITVPPAGFIAGVYARTDVDRGVHKAPANET
                     VLGALSFEQDINQFQQELLNPNGINCLRAFPGRGLRVWGGRTLSSDPEWKYVNVRRYF
                     LYLERSIEKSTQWAVFEPNGEALWANIRSSVEDFLFNEWKNGRLLGGAPKEAYFVRCD
                     RSTMTQNDLDNGRLVCVVGVAPLRPAEFVIFRIGQKTADA"
     gene            complement(24733..25548)
                     /locus_tag="PKX01_06050"
     CDS             complement(24733..25548)
                     /locus_tag="PKX01_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75722.1"
                     /translation="MILETVIVHGSDFIEWLDASGNAGGTARRIAPARLRVELTKAPR
                     NLRIAHKPRATALLRTTDTPLPPAKEPARLALVVPGEATPAQRVPPAQNPYQIEGIVR
                     DPAGEFLPRRFSLNGGTAAGHQIRLFRSPLGSRYGSAGGVFGRLTFADDAPAAWALVE
                     LSVKPPLGATQTYTAQADAHGEFILPLDRLPAVAVDAVVKSYPATLTVLASATTDPNT
                     PFDPDELVACDVATGKDAGGQPEFAPSLSFTFTPGAISRVVSPEHSLLRLKSN"
     gene            complement(25545..26135)
                     /locus_tag="PKX01_06055"
     CDS             complement(25545..26135)
                     /locus_tag="PKX01_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HMZ75723.1"
                     /translation="MAGHRAVLGVLQALKERLDVRMPPLTGGSPKALVLGSQTLAAGP
                     SGENLGIYLHRIAIDPFARNRYLAPAPPQRTPRPELPVNLHVLLIGWSVKNETEIGYL
                     AAAMQIIGGALTLDVSHLASADATWDDHDSVQVMPEEMSTEDLMRLWDSLPGDYRLST
                     PYLIKTLRLAPDLSADEGPLVRSVLIPAGTLPEDAP"
     gene            complement(26286..26690)
                     /locus_tag="PKX01_06060"
     CDS             complement(26286..26690)
                     /locus_tag="PKX01_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ75724.1"
                     /translation="MTITVTRDTSGPMRHIVSVGRHRLTVDEPPDVGGEDAGPNPHDL
                     YDAALGACKALTLVWYARRKGIPLDAVEIEVERDASHERDGHYRLSTRLTLGGPLDEA
                     QRAQLLAVAARCPVHKLMTQVSTEIETTLKNG"
     gene            complement(26738..27634)
                     /locus_tag="PKX01_06065"
     CDS             complement(26738..27634)
                     /locus_tag="PKX01_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ75725.1"
                     /translation="MNFPAPPRAIAAIEREGILHPRTVEHLITGQPTRDGAGVTLNRL
                     ITPALQRRLDPFLMLDAFGSDSPDDYLAGFPDHPHRGFETITFMIEGRMRHRDSVGHE
                     GVLTSGGVQWMVAGRGVIHSELPEQQDGRMEGFQLWLNLAARDKLCPPWYRDIAAADL
                     PVYTTPDGVRVQIIAGRSHGIHGAVERPVTEPLYLDIRMTAGSHFAQPLPAGHNAFVC
                     VYRGQVCCGAADTEVAAGRMAILETNPDADGVLLTAKAPTRLILVAGRPLGEPIVQYG
                     PFVMNTEQEIRQTLEDFRAGRF"
     gene            27756..28811
                     /locus_tag="PKX01_06070"
     CDS             27756..28811
                     /locus_tag="PKX01_06070"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMZ75726.1"
                     /translation="MARSSSVSFEAVEQICFQLLSQGENPSFNLVYAELGNKGSSEIV
                     QRYIDQWRRETGAAFFRQRSVPGMPDGLVTATDRFMAEVWQMSLHHAEQLLESRRVEL
                     EQEREAIRAQLQLAHDQIGAQNAEAVRVAQQLETLERKAEACEITIGDLRAQNGDLMR
                     QREALETRVRLLEEAAETREQKFTAESERLAKLLQTDRDRYNASLKEEQERGAVEREH
                     LMKQIDVARQEKLAEITSMRQQLATREERETRQREQAEEARAALREAQSRARQMETSL
                     AAAEGRIERLQAQLKTASDAAHGKESAVAAAEARARAMEDVRIAETARAQRAEAALAE
                     TLAELGILRKKMAAGGA"
     gene            complement(28827..29996)
                     /gene="prpF"
                     /locus_tag="PKX01_06075"
     CDS             complement(28827..29996)
                     /gene="prpF"
                     /locus_tag="PKX01_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HMZ75727.1"
                     /translation="MTRFPPPVRPCRIPACLMRGGTSKGLFFLEQDLPDDPDLRDRLL
                     IRAVGSPDPYRQHMDGLGGATSSTSKVVLVGPSTHPGCDVNYRFGAVAVDRAVIDWSG
                     NCGNLSAAVAPFALHRGLVRVPRDGVAVVRIWQADIRKVIVAHIPVADGAVVEDGDFQ
                     LDGVAFPGAEIRLDFLDPAGDGEALFPTGHRRDTLSVAGLGNVDATLINAGNPTVFVH
                     ADTLGLTGTELQRDVNGQPELLARAEALRAAAAVAMGLAPTADEATRNRPHTPKLAYV
                     AAPAPYVASDGRQIVAGEVDLLVRIFSMGPLHHAMTGTGAVAIAVAAAIPGTLVHEVA
                     RVNAGGRVCFGHPSGTLTAGADAQIDAAGNWVIRQVSMSRSARRLMDGQVFIPAV"
     gene            complement(30193..30753)
                     /locus_tag="PKX01_06080"
     CDS             complement(30193..30753)
                     /locus_tag="PKX01_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HMZ75728.1"
                     /translation="MAEIYVSTDVETDGPVAGIHSMLSLGSAAFTADKRLLTTFSANL
                     ETIPGCVADPVTTAWWKTQPEAWAACRQDPEAPVRAMTRYLDWLKALPGRPVFVAYPS
                     AFDFPFVYYYLMRFTGENPFGYSAIDIKTYAMAMLRRAYRGSGKHAMPDRWFDPSPHT
                     HVALDDALEQGRLFCNMLADNLARRA"
     gene            complement(30763..>32894)
                     /locus_tag="PKX01_06085"
     CDS             complement(30763..>32894)
                     /locus_tag="PKX01_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ75729.1"
                     /translation="WTHVRKDGSRIQVEMSVRELEFDGHRAATVIAHDMTRRLEAEAA
                     LRKLSQVVEQSPDSIVITNAAGRIEYVNDAFLHSSGRTRDTTVGQHARILKSGLTPDT
                     TYAALRTAMAAGQAWKGEFVSRHCDGTEYVELALITPIRQPDGTVSHFVAIKEDITEK
                     RRLGAELERYRHHLEELVAERTSALRATTAKLLDTQFAMDSVGIGIHWVDADSGRFTY
                     VNRFSAGLLGYTEQEMLQLSVPDVDPHFPPDAYRAIAERIRREGRIQIETAERHKDGH
                     PVPVELTIYHLAGREGGERGRFIVFLTEITRRKEAEAALHQAKSAAVAANQAKSAFLA
                     NMSHEIRTPMNAILGFTRLLQRSPLSAEQTEQVDKIGAAGEHLLSIINDVLDLSKIEA
                     DKLHLEETDFPASALLDGVRSLIGELAQTKGLEVTIDYGNLPTYLRGDPTRLRQALLN
                     YASNAIKFTARGSIHLAAHVLVEEGDALLVRFEVRDSGVGIAPEKLGQLFQAFSQVDS
                     STTRRFGGTGLGLAITHRLAGLMGGETGVDSTPGQGSLFWFTARLKRGRPVAEALRTR
                     TPEDAEVQLRARHAGARILLVEDDAINQEVALSLLADTGLVVDVAENGARAVAMAGAA
                     PYELILMDMQMPVMDGLAATRALRAQPAMQQTPIIAMTANAFAEDRRHCLAVGMNDFL
                     PKPVEPDALFGMLLRWLPDGDGSQGTR"
BASE COUNT         4851 a        10009 c        13020 g         5014 t
ORIGIN      
        1 gtgagggaag ccatggttca ttccccgatc tgggccagct ggggcggcgg ggtgcggccc
       61 agcttgcggt attcctcggc gagcccggcc accagcaggg tgatctcgac cggcgtgccg
      121 ggcgggcggg ggctgagggc gacggcgtac agcacggcat tgcggatatg gccgccgggc
      181 aggtcactgt aggtggcgag caggccgcag gcggcttcgc cggggctgcg ggcgcccaga
      241 tgggcgtgcc acaggcgcag gcgctcggcc acgcccggca gcggaaattc gatgatggtg
      301 tcgaagcgcc gggtgaaggc ggcgtcgagg cggttgcggg cgttggtggt gaggatgacg
      361 atgcccgggt ggctttcgat gcgggtgagc aggaagttgg tcatcatgtt ggcgtaacgc
      421 tcgccgccat ccttgccctc gctgcgtcgg ccaaagaggg cgtcggcctc atccatcagg
      481 agcagcgcgt ccagggtggc ggcctcgtcc agcaggcgcc ccaggttctt ctccgtctcg
      541 cccacgtatt tgttgaggat ggcggccaga tccacccgat agagcggtgc gcccagctcg
      601 gtggcgaggc ggctggcggc gagggtcttg ccggcaccgc tctccccggc gaagagggcg
      661 cgcacgcccg gtgcggggct ggcaaggctg gggccaaggc cgtcccacag gcgttcgcgc
      721 atgcggcagc ggcgtacaag atgttcgaac tggcgggccg tctcggcggg caggacaagt
      781 ccatcggggc ccacgtcgcg gtgcaccggt tgggccagct ggcgcaggcg ttcggcgccg
      841 tgggtgctgc gggcgcggcg caggtgggcg agtgccggca tctcgtggcg gcgcgcggcg
      901 tcgcggccca cggcctgggc cagggcgtga atggcggggc cgtccagccg ggcgtgatcg
      961 gcgaggacgg gcggggcgtc cgggcccagg acggcctgcc aggcgcggtg ccgctcgtgc
     1021 cgttcgaggg gcgggatgtc cagttcgatg gcgtcgggcg tatccacgct gccgtcctga
     1081 ccggtgacga ggacctgggg cgtctgctca agcgagcccg tgggcaggct gaaggtgtcg
     1141 ccgggcccca ggtctgtgag gaagacgggc agccagcccc cgtagcggca ggccgcggcg
     1201 aagatcgggg tgctgcgcca ggggtcgggt ggaatggtca ctggaagcag ccccagggcc
     1261 gcgccaaggc gtgccgcagc cgcccgaccg gtgccgtggc tgccccgcag gacgacgagg
     1321 cgggcagcgc cctgctgcac caggtgcgcg gctgcgtcca gttcggtgag cagggtggcg
     1381 ggtagcacgt gcgtgagggt gtccggcggc agcggcgtga agtctgccca cgggcgtcgg
     1441 tctccggcca gcagggcggc cagacgcggg tctacacgca ggctggtacg gaccagggga
     1501 aggtcgccgg acaggctcgc caggccgcgc tggacgaggg cgtggtgggg ccacgcgagg
     1561 ggcggaaggg ccgcgtcgaa gagcgtcgcg gcgagggcgt cgaggaggtg cagggcgggt
     1621 cgcccgtccg cgtcgggggc ctgcagctcg gcgagggccc ggccgacggc gtgatccgcc
     1681 tccacttcgg cggccagggc cagggcgaac cattcaggca gctgaaggcg gtagtcggtc
     1741 aggagggcgc cccaggcacc gccaaggctc ggggctcggg cggccagggc cgcgcaggcg
     1801 gcggcccagt cgtccgggga actgtccagc aggcaatgca agccggcatc ggccgcgagc
     1861 tcggcaaaga gaccggaggg cgcagcgagt cgggtgagtc cgaaacgtgc cagaccggcc
     1921 cggacgaagg cgcggttgag gaccgctcca tccagcgcgc gggcggacgc gagcggggcg
     1981 ttcatggtcg ttccccgggc tttttggcgg ctgcgggcgc agcgggtccg gtgtcgggag
     2041 ggccggcggg cgaaagggcg ggcgagtggt cgcgccccat cgtgaagtct tcgggcttgc
     2101 ccattgggga gggcagtgca cgcgggccgt cgttgaagtg gaaggtgacc acccggccca
     2161 gccacggcag ccagccgggg ttgaggtcga gcccggcgcg ccgcagatcg aggcgcgcgt
     2221 cggcaaggcg gaagtgggcg tccacgtggc tgggggtggc gacaatccgg gcgggcaggc
     2281 ggaaggcgtc agcgcgccac agatccgcgc catagcgggc tgcggcgaag gcctccaggc
     2341 gcgcctcttc ggccagcggc gggaggacgg cgagggcatc cggactggcg agggcggctt
     2401 cggtcgccaa gaacgcggcc agcggtgggt ccggctcgag tccgagggtg gcggccagac
     2461 gcgccagcca gcgccagccg cagcccggga ggttccggtc tccgagcagg ctctgcaccg
     2521 gcgtgagggc aaggacgttc agcaggaaga gcagaccgcc gcacgaggtg acaaagccgt
     2581 cttcaggggc ggcggcgcgc ggtgaatgga gggcggctgc ggcgcattcg gcggccgggg
     2641 cgtcggatgc gggctcgcgc gacgcatcgg atgtcgtggt ggtccgcccg ggatgcgcgc
     2701 cggtggtgcg cgcctcgacg ggagcggccg tcggcagcgg cgcagggtgc ggtgatggcg
     2761 tgtcggcagg cgcaggcact gtggcgtccc gggcgatggg catcggctgc tgcggggcgc
     2821 tccgggcgcc atcgtgggtg acgaggtgcg tgaggcgggt cagcaggacg tcggcgtccg
     2881 gtgtgcccag gtgcgcggga acgtggcgcc aggcgagcag cacggccacg aggcgcgccg
     2941 cgggggccgc gggcgcgagg cgaagggcgt gggcctgccg tgtggcggtg gcgagcaggg
     3001 cggtcgggag gccgggcgca ggcgccatgg cggtggcctc gggggccgca aaacgcagcg
     3061 accacccggt ggccgcgcgc agggcgccga gcaggcgctc ggtggccggc gcgtccaggc
     3121 gggccatcag gcgcggcagg agttcgatgg ccgcgagggg acgggcgcgg gcggacccgg
     3181 catcgagcag ttcgggcagt tcgaagggcg cctcttcgag gatccggacg atggcgtccg
     3241 ggacgggccg ttcgaacagt tcggcccggc gtgcccagaa ccagcgatcg gcggcgcggc
     3301 cgtccagcag gtcggcgagc agacaggcgg acatgtccgc gcgggatcgg aagcgcaccg
     3361 cgtcggcctg gtcggcggcc cggctccagc cgtcctcggc acggggcgtg agttcgcgga
     3421 cgaggtcggc agcacgacgg gcgatgtcgg tggcggagcc gcgcaggcgc aggtggcgca
     3481 cgaagaggag ggtgtccggc cccgggtcgg gccacggtgc ccgatccagc gcggccagca
     3541 ggggcgtccg tgcccgcgcc acgtcgtccc gggcgccgcg caggttcagg cgggcgatgg
     3601 tgacggtggc ggaggcgcgg tccatggggc ggtgtccggg ctagggattg cggctgccgg
     3661 tcacacggaa tgccgtggtg aggggccggt tcacgccgag gctgtcgtcc agcacgcgga
     3721 cgatggaggc caccgtctgg ccgttgtcgt ggtcgtccac ccgcagcgtg ccgaccagcc
     3781 gctgggcatc gtcgaggagc tgcagcaggg cgcccgggcg gggctcgcgg ttgagttcga
     3841 ggcgcatgac ctcgccgatg cggaagcggc gtggcgggct gctgacgtga ccctggagga
     3901 tctcgtccgg gttgtcgccg gtggcgagca tgacgcaggc acggacgaag ttgcgcatgg
     3961 ccgtgcggga cgcggcgctg gcgtcgctgt cgagctggcg gaaccaccag gcaacgagct
     4021 caccgaatgt acgcaggccg ttgaggtcgg cggcttcggc ctgggcgatg ccgggccagc
     4081 gttccgggcg ctccacgggc agggtgtcgg cctcggcggc ggcggcccag gccaggcgca
     4141 gggacggggc aatggggcgc agcaggtcca gcagcccgcc ggcggcggca tcgaggcggc
     4201 ggtgcagcac gcgggcgggt tcgcgcagca gaggaaccgg gctggcaagg aggtcatcga
     4261 gggccaggat ggcgtggccc tggcgttgca ggtcgagcat ggcgctggcg gcaaaggggc
     4321 gcagggcgat gtcgtcgagg agtccgcggt tctggaaggc gagcccctgc agacgcgcgg
     4381 tggcgacggg caggccgatc ttctggcgca gggcgacctt ctgcaggcgc gtggcgacca
     4441 tcagttccag acgcgggcgg ctcggcaaca ggtgggccag gcccgacagc acggcccagt
     4501 ccgcgacggg gatgtcgagg acgacctcca tgaagggcgc gaggtcgtcg gcgagcggaa
     4561 tctcccggtc gggcacgccg gggacgatat cgtccggcga ggtgaagcgc aggtcctggg
     4621 ggtcgggcag ggtcacggtg accggcgtct cgcgctgttt gacgtagaac agtccccggt
     4681 ggctgtcgat gacgcggcgg cgttcggcgt aagcctgcgc gacggcgtcc cagtgctcgg
     4741 ccagggtgtg ctgggcagcg gcgtagtcgt cgagggcttc gaggcgggtg tggttgaggg
     4801 tgtcgaggcg ggcggcggcc tccgggagct gggtttcgac ggtggcgagc tggtcctgga
     4861 gggccgcgag ctggttctgg tggtcctggc gcagggccac caggacggcg cgcagggctt
     4921 cgatgccgcg gatgtcgtcg gtgatcacgg cgccgtggtg gaacagggcc gagtgggcgt
     4981 tatgggtggc gctcttgctg tcggcgtcag tgaaggtggc gccgatcttg gggggcagcg
     5041 tggtgaacag gttgctgaag gcggcgatgc cgtgctggcc caggcgcgcg ttgagcgcgt
     5101 cctcgctgcc cagctgatcg atgaggaagc gacggacgcg ggccaggagc gtggtggtgg
     5161 tgtcgagggt gggtttgccg tcttcgccca gggcgatggc gatgcgccgc agggcggtga
     5221 gattctcgag ggccgtcgag tagtagttct gcaccacgtc gaactggcgg atggtgccga
     5281 ggctctgggt ggtttgggca atggtgccga tggcgatggg atcggccggg cgggcggcgg
     5341 cgctggcggc ggagctttcg acggcctggg cggcgacggc gaaggcggcg atcgcctggg
     5401 tctggggctc gagggtcagg aacgcctgct gggtggaaag tttttccacc tggggcagcg
     5461 cggcgctggg tttgacgccc acctggagct tgaggtcggc gatgttgtcg aagacgccgg
     5521 atttgttgag caggttcatg cgtgtctccc ggccctattt tgtgctggcg ggcgtgtagc
     5581 tggccgcgtt ggtccagcgc atcaccttga gccccgagcc gtcgccggcg acgccgccgg
     5641 ccagggcgct gaaggacacg gtgagcgcgt cgaagtgctg gcgttgcagc cccaggtagt
     5701 cgttgagctc gttgaggagc ttgtagctgg tttcgaggcg ggtctgcatg tccttgatct
     5761 gggcgcgcag gtcggcgcgc tggcggagca ggccgtcggt ggtgtcggtt tcaggcaggg
     5821 ctgcgggaac cgcctcgtaa cgcggcgggt gatcgtcaag ccagaagacc gggtcatcgg
     5881 ccatcttgcc ggcgctgaag gcatcgacga gcaggctgcg gcgggcgccc cgaaaggcgt
     5941 ccgggctgac cggcggacgg acttcttcga cgcgcgcccg ggtggcgctc cgctccggcc
     6001 gggcaagctc cgcggcaacg ggttcctggt ccgccacgtc gccgggcaga cgcggctggg
     6061 tgccctgcag cagggtgtcg aggagttcga cgggcgccag gtttccgaac accttctgcc
     6121 actggttgcg ccaggcctgg cgtgcagcct cggcggtgtc gaagcgttcg cacagggtgg
     6181 cttcggcccg cacgtcacgc aggggcaggt cgaggagatc tggccgatag tccaggtcgg
     6241 gaatggcgag catcaggcgc agacggtcgc ggcgggggtg gaggaggtcc acgtagccac
     6301 gggaaagttc ttcggcaatg atgccctcca cggtgttggc cggcaccggg atgaggtcga
     6361 tgcccagatc ggacggattg aaatcgagct ggggaggatg gtcggccggg ttgcgcagca
     6421 gggcaggcgg cagcgcgccg gccgcgggca ggtaggacag gccgaggcgt tcggtcagca
     6481 ggaagcccgc ggggctggcg tccgatccca ggggctgcag gccggcctgc agcaccgaca
     6541 gggtgtgggc aaggaaggtg cgatggggcg cgtccgcttc cgccatgaag cggcccccga
     6601 ccgattcgag ccacgccagc tggcggtctg cgaccttgac catgcccacc ggcacggcgc
     6661 cgaccgccag ggtgaagggg gaggcgtcga ggaagcccac gctcaggcgg ttgacggtct
     6721 cctgctgggg cagcgagagc aggcgcgggc tgcgggacag ctcgaccaga cccggcaggg
     6781 tgacaacctc cagacggcgt tcgcgggtgg ggtctgcttc ggtgcggatg gccgggctgc
     6841 gcgtgtcgag cgcatcgacc tcttccacga cgctgcgcag ggtgaggaga tagagaccat
     6901 ccttcagtgg cgcattgccc cggtcacgtt ccgcaagggt gaggagatcg ctccaggcag
     6961 tgaccagcgg tgtgtagagg cgaaccagct gacctgcgcc ggtgacgccc cgcccgggcc
     7021 tgaaacgcag gcggggcgca cgggccgcct cctcgaggct cacctcgaag cccgcgagga
     7081 tgccgggctg aaggcgcccg gcgaggggcg ccatgcggtc gtccgtgtag tcctggagct
     7141 tgcgaaaggc ctgctccggc agggcaaggc ccggaaagag gttgagccgg cgcggatcgt
     7201 ccggtccggc ctgcggagat tcgacgtgga tcgacgcagt catggaaagc tcctgtccgc
     7261 gggggcgggg tcagcgctgg ttggcccact ggttgatgag gttcggggac agtcccgcgt
     7321 tctggcccgc ctggaccatg gccttgggga cggtcatgcg ctggacggtg agcaactgga
     7381 ccagttcgtc ccgtagcttg ggcagccgat ccggctgacg cgcgagatcc tgcaggcttg
     7441 gccacaggac ctcgtcgtat tcgacgccga tcagcaggaa caggtcgtag gcggcattca
     7501 gcacttcgct cggccgaccc cgcaactgat cgtcgagctt gaggcccaga tccatttggg
     7561 cgccacggac ccgcgccaca tcggccagcg tgacgccgga ggcattggga ttggcgcgca
     7621 ggtcggccaa ttgacgttgc aggagctgga tctgattgtt cagggcgttg atctgcgacg
     7681 cctgctggtt gatctgagcg gtgagctggg cctgggtggc ctgatccgcg gcgatctggt
     7741 tgcgcaggcc ggcctggatc tgggcctggt tgctgagctc gacgttgagc ccggcaacct
     7801 ggccgcgcag ctggtcggcg tcaccgcggg ccacggtgag ggcgttttgc gcggtggtga
     7861 cgtcgttctg gagaccaccg atgcggttgt tggccgcgtc cagggcggca ctggtcgcgc
     7921 tgagccgggc gttgctggcg ttcagtgcat cgcgggcacc cgccagatcc gattcggtgc
     7981 tggcgacccg tccgttggcg gagttgagct ggttctggag atcgtcggcg cgggcctgtg
     8041 cggccgtcgc ctggttggtg gcggcttcgg ccgcggttcg ctgcgcgttg agctggctgg
     8101 tcgtatcggc gagctgttgg cgggccgtgt cggcggcggc ctgggccgcc gccaggtcgg
     8161 tggtgagctg ggcgacgcgc tgatcggggt tttcgtcgat acgggcctgg agctgggtga
     8221 tcgtggtgtt gagttccgcg acggtgccct gcagcgcggt ggcgcgggcg tccgcttcgt
     8281 cggcgcggga ggtggcgctc tgcaggtcgc cggtaagggt gtcgttggtg gttttcaggt
     8341 tggcaaggtc gcccgtcagc ctggcgttcg catcggtgag gctggtgttc gtggcattga
     8401 ggctgtccac ctggttcctg gcggtggtca ggtctccgct cgccgtggcc agttgggctt
     8461 ccaggtcctg gatgtggggg cgctgttggg agagttcgac ctggagtgcc tggatgtccg
     8521 tgagggcctg gtcgagctgg tgttccgaat cgagcagatc ccgctggggc agggtgatct
     8581 cttcggggat tgcaaagccg cgggcgagga cgacggccga gacattggtc tcggcggtgc
     8641 ggggtgggcg gcggacatag cgcacgtcgg atggggcgac cgcagcccag atctgttccc
     8701 aggtccggcg gtcctcggtg ccgagggccg gtggtgcgta caggcgcagg gcgaagacgt
     8761 ccagatgggc gaggatgccc gtcttgccga gggtcccggt gaccggatcg gcctccagct
     8821 ggcgggcgcg cagggcgaag ctggcgtcgt ccatggggac caggaccatc acgtcgatgt
     8881 ccgcatcgcg ctccagatcg atgggcgcaa gcaaggcgga gtcggccagc aggcgcggca
     8941 ggtcgctgcg cctcacgggc gcaatgctca cctcgtaggc cttgggaaag aagatctggg
     9001 tgccgttcac cgggtcgatg gcggccttgg gcagcgggcc atgggcgggc agcatgcgga
     9061 aatactgggc ggccggaaag ccgtcgccca atccggcgtg gctgcgggcg tcgagcacgt
     9121 cgtggagcag ctcgacgtaa tgcagggcca ggtcttctcc ctgcgcggcg ggcgccagcg
     9181 ggttgcgcag gcggcggcgt acgaggccct tgtcgtacca ggcgacgacg gcctgttcga
     9241 tggccacgag gccgagggcg atggcgtcgt cggagggcag catgaggctg ccgctggcgt
     9301 ggaggatctc gcgggccagg gtggcgcgca ggctcagggc gtcaccccgg ggcaggccga
     9361 tgggcagggg cacgaggacc agctcgacgc cctcggcgta ctcgctgacc ttccactggc
     9421 ggcggctttc caggtcggtg gggtaggcct ccgcgacgcc actggcgact tcggcatgct
     9481 gcaggacgac ggcgtagagt ccccgcggaa actggcggta ggcgccgttg ttgaggctcg
     9541 cgatgagggc gctgttctgc aggtcggctt cgagcacccg cccgctgagc ttgagcaccc
     9601 gtccggacgg ggcgatggcg aggccgggct gcacgcggac gtagcggccg tcggcaatgt
     9661 ccaggtccag cccctcggcg atgcctgcgc cgaaggcctg gccgagttcc agcaggcgct
     9721 cgtccaggta gatctggtcg cgggtgaggt ccgtggcctt gaggagctgc ccgtcaaaat
     9781 agttggtgcg ggacaggcgc gggtcgatgc tgcccaggcg ggcgccgccg gcggtggcgg
     9841 tggtgccggg agcgtagggc tggaactgga gcgtgttcgc catgatgacc tcactgacgg
     9901 agcagacgag ccagggtgct ggcgttgaag aggaagggcc gggccaggtt gtccacgacg
     9961 atgcgcgccg ggtcgatgac gagctggccg cccgggtcga gggcgatggt gaccgttgcg
    10021 agcagggttc tggccagttc ggctgcggtc acggcgaggg actcgcgggt ggcgagggcg
    10081 gtgttgtcgt cggacagggc gaacagcagg cgggcgccaa gctggagctg gcggcgcccg
    10141 gcggcatcgg cggcggcgat ggcggcacgt tcgtggtcgg ccaggcggtc cgcgatgtcg
    10201 tcgtaggcgg agaggtcgtc gtgggcggtg aagggccttt cggggacgtc gccgatggcg
    10261 ggccgccacg gggtgagttc gtaaagcacc gtgtcctgaa tgcggctggg accgaccggg
    10321 tcggtgccgg cattgatcgc cgtcgccatc accggctgga gcccggaggg cacgtcctgg
    10381 tagcgcatgg tgatgcgcag gtgcagggtg gtgccggcgg tgtcgagacc gtcccgttcc
    10441 agggcgcccc aggcggtgct gtcggcgtgc tgggcggtga gccagtcgcc caggtcgatg
    10501 gcgtagggtt cgtggacgct cacctcgcgg ccgaggccgt ccacgccgat gcccggggtg
    10561 atgatgaggc gcacccgcca cggctttccg gcggtcttgg gcggcgtcga gtccatcacc
    10621 acgcgcaggc cggcgaccgt accggcgccg tgcagccagt aggcgtggcg ggtgtggcgg
    10681 cggcggtggt aggcctgctc ggcgcgggtc gcctcgatgc cgagcagcat gccgggttca
    10741 aagctgacgc ggcgcagcgg ctcgctgaag acgccgtcgt cgggcaggct ggaatcggtg
    10801 gccgcgttgt cgtcggactg gctcatgccc ggtctccttc ggattcgggg ggcggcagcg
    10861 aaccggcctg ttcgggcagc agggccaccg ggttgtagat gaggtcgccg cggcccaggc
    10921 gggtggtgtc aagccggacc ggcttcggcg gcggctgggt ctcgagataa gtgtcgatgc
    10981 gcagcagggg cgacaggccg agcacgaagg ggtgctcgct gaccagtacg ttccattgca
    11041 cgatggccgg gacgagctcc ggcagggcct cctcgatgac gccgcgcagg gactgggcgg
    11101 ggccgtgcag gatcaccgtg aggcggcggg cgtagcggtc gaaaaagtgt tcgacggcct
    11161 ggcgctcggt gccggggcgg gccagttcgg gggcgaacag ggcgaggatc tccacggcgt
    11221 ggtcctcgga cagcaccagg gtgtcgccga cgaggctgtt gccggactgg ccggtgccca
    11281 gggtgagggg atggcgcgca tcgtccatgg acagacccag taccgtggcc agcgtgcggc
    11341 gcaggcggaa gagttcgacc ggcaccactt cgccacggct cacgccccct tccgtgagaa
    11401 tgtcgagggc ctggcacagg gcaccgtagc tgcccttgtg gggctgcaga cggccaaggg
    11461 cggcaagcca gcggcgctgg cgggtctctg gccagtgggg acgcaggcgg gtgccgagca
    11521 tggcggcgag attggccagc cacggggccg gcacggccag cgggtcgagg agttgttcgc
    11581 tggcggcgat gcggtcttca atgggggtga gcaggccgct gaagctggcg aagaggcgtt
    11641 cgcgcaggtc ggcgccgttg gccgcccgcc cggccggaac cggttccgga tcggcgggct
    11701 gctgctggtg gaagtggtcc ggcaggtagt gggcctgcca ggaaaagcgc gggtaccaga
    11761 ttctcagggc gtacacggcg ggactgtggc gaccgtcgcc gccaagcgtc aggcgcaggc
    11821 gcagataacg cccgcggagt tcacgggcgg cgccgctgct gcgctggagg aggatctcgt
    11881 ggagtgttcc gcgcggtgtc gcgaagggga tgtcaggggt cgtgagggcc aggggcaggg
    11941 gctggcggtg gaaggtgtcg gcaaatccgc cgggcgtgtc cgctgcctgg gcttcgacca
    12001 tgaccgagca gccagccgga atgcaggcgt cgaggatgag gcggtcccag acggtgtccg
    12061 gcgtgccggc atcgaaaacc ggggtcagca gggcgcggcc gtgggggagg aagcgcgcct
    12121 gggcaaggcg gaacaggcgc accacgccgt cgttggccag atgccgcact tggttgtccc
    12181 gatgacgcac gaagcgcacg gcggcttcgc tgcggcgcgg ccagcgctcg gcggcgaggg
    12241 tgggcgggtc gtcgagggtg atcaccgggc agtcgcgccg ctgggcccgc gtgacgccgg
    12301 tttcaagcgg cggcaggagg atcgcccggc cgtcgtcgag ggcggcgatg tcactggcga
    12361 aggggaggcc ggtggggagc gggagggccg tgagcgcagc cgtcgggtcg tcggacaggg
    12421 cgcggacgag gaggatctgc tgcggtgcgc cgtcagtgct cccggacgct tcggcgagga
    12481 tcagcagccg ggtggcgacc acggcgatgg cgttgatgcg gcggagcggc ggcagcgcct
    12541 gggcccaccg tacgcgcggt ggcgacggtt cgacggagac cggttcgaag cggtctggac
    12601 gcggaacata gggcgggtcg aggggggcgc cgatggtcag ggtcaggtgg gtgtcgctgg
    12661 cgacccatac ccggtcagag gcgtcgaccc agagccggac cggccggaag gccggcgcct
    12721 ccggcgacgc gatctccagg tgtcgctgcc agcgggcgcg cagatggaca agggtgacgc
    12781 cggcgcgggt gccgtcgtcc gccatccagc tggcggcgag caggccgctg ccgcccaggt
    12841 gcaggtcggt gaaggcgcac gcggtgggcg cgtcgaccgg gtcgagcgtg agggcttcca
    12901 ggtcggcggc gaggggggcg gtggggtcgc gatgggcacg cagggggttc cacggggcat
    12961 cgggccagcc gacggcggct tcgatgctgc gccggtcggc ggacagccgg gcgatctggc
    13021 cgtagtcgtc cagaatccac ggccctgcat cggcccaggc ctggagcgcg tcagccgccg
    13081 ccagacgggg caggcggggc tcgtcgtgac ggaccagggt gaaggcgcgg gcgtctgcat
    13141 cccaggcaca gctgccgccc gcgttgcgga aatccgccgc gcggtcgagg agcaggaagc
    13201 gggtgccgtt gctgtccatg gttcagcaga cgtccgggat caccggtacc gggacggcac
    13261 gggtgcttcc gccggtcgag gtgccgggcg cgaaggcgcg gggcggggcc ggtgtggctt
    13321 cgccggactg gacggcgacg gccatcagtt cgggaagctg gtagtcggtg aggggcaggg
    13381 acagccgccc ggtgagttcg cgccagaggc cgctggcgag atcctggtag aacaggcgca
    13441 ggccgttgac cgcctcgatg ccgtccacac ggctggcctg ggtgcgcagc tcgttgatgt
    13501 ccacggcgcg cccccggggc cagcctgcgc cacgcacgcc gtggggcggc agcggccaca
    13561 ggtaggcgag gagggcctgc tcgacggcgc ggaaggtctg ctgctccagg gcggggtcca
    13621 ccacttcgac ggagaccgcg acggagaccg gctggtactg gaggctcagc acgtagagct
    13681 cggtgcccag cagggtgcgc ggagagaggt attcgaagac atcccgcagg gtgccggcgg
    13741 tgggccgcgg gaaggcggcg aaggcgggtt cggccggggg caggacgaag acgctgatga
    13801 cgcccggtac gttgcgccgc actgcggtga ggctggcgcc gggcaggaag ccggcggcgg
    13861 cgtcggcgcg ggcgatcggg cggagcggat tgtcgcgggc caggacggcg aagtcctcgg
    13921 cggtgacggc ccgttcccga tgggccagga aggcggggat gcgctgctcg gcctcggcca
    13981 cggtctcggc atccacgccg ccgcgcaggg gccattcctg ccgcggcttg aggcgcccgc
    14041 caccgacggc gacggactgg accgcaccgg gcgcgaggtt gcctgccgtg ccaccgccaa
    14101 agcggtagta cgaggcccgg atgcgcaggc cgtcagacgg acggcggccg cgcaggccgt
    14161 cgccgaagcg cacgctgccg ctgtcgccgt ccacgaggta gaccgcgtcg tccgggccgg
    14221 cggcaccgaa atggtccacc tgctgccagg gctcgaatcg gccggcggat tccacgtcga
    14281 tgcgcacgtc ggacgtgtcc acggcggcgt ccggcagggc aagactctgt tcgggccggc
    14341 cggtgccgat acccagcatc acgtcgcggg cgacgccctg ggcgaggacg tccacgccat
    14401 tgacgcccag gtagcccagc tcgatgccgt cgccgtcggc gctgcgcacg cgcagccagc
    14461 actggagctg gccggcggcc aggtcggcgg gggcttccgg gggcgtgtcg cccatgcccg
    14521 cgaactgggg gtcgatggcg ctcggaacga ccaggaagct gccgtcgcgg ggcaggcgca
    14581 ggcgcaccac gccggcctgg cgaccgccgc gggaggagtc ggcgacggtc tcgaggggca
    14641 gccagacgac gctctcggga tggttcgcgg gatcgcgcca gactgccacg tcccagtcga
    14701 ggcgacgggg cgacaggctg gcggccagct cgccgtccag gtcgtcggcg ggcgccaggg
    14761 ccaggttgag gaccacgccg gcaagcaggc ggcggacggc gtcggccttg cgcaccagcg
    14821 ccttgggcac ggcgaaggcg aggtagaggc tgtcgtccag gctgccggcc gtggtgaccg
    14881 tgtcggccgg cggcgtgagg cgcaccgggc ggaaggcggc cggttcgaca ccgtattgct
    14941 ggcgcagctg ggccagggag atgccttcgg cgctgagggt ggcggcgtcc agcgtgcgtt
    15001 tgacgaggac gtgcagttcc aggggcaggg gctggagttc gcccacggtg gtgagggcga
    15061 tctggcccgc ggcgagggcc gtctcggtgc gcaccagcgg cggcaggctg cggccatcgc
    15121 cgccggaagg atcgatgcac agcacgccgc gggcggggcg cgcggggcgc aggggcaggg
    15181 ccaggaggtt gaggaaggcg aggcgctggc ggtcggggat gcggttggcg cggtagagga
    15241 tggtctcagt gagccaggcg aagaggtcca ccagcgcaaa ggccgggtcg ccgggggcca
    15301 gggcggtaag ttcgggcacc tggcgctgga gccgctgctg cagttcggcg acgaggtcgt
    15361 cgaagcggcg gtcgtccaga tcgggaaggg gcaggggcat gatggtcgtc ctaggcaccg
    15421 agttcgatgg ccagatcaag gctgtcccgg cggccgtcct ggcgcaggcg gtagcggatg
    15481 gacaggttga tgtgggacag gcgttgcggg tcgggcacca cgcggacctc ctcgatgctc
    15541 acgcgcggtt cccagcggac gagggcacgc tgcaccgcgt cggcgacgag ggcgcgggtc
    15601 gtctcgttgt tggggtggtg gatgaagtcc cgcaggccgg cgccgaagtc cgggcgcagc
    15661 aggcgttcgc cggggcgcgt gaggaggatg ttgagcatca cctcgcggat gctcttgtcc
    15721 ccgtcggccc acgcgagacg gccatcgggc gcgacgccgt cgaggagggg aaagctgagg
    15781 aggcggggcg cggaggaggc catggctcag tccttgagga gcttgccgca caggcggggc
    15841 agggccagca gggcccaggg cagccagccg aagatcaggt tgagcaggtt gatgaggatc
    15901 atcaggatga tcatcgcgca aatggtgaca atggggatgc tcaggctgaa gacccagcac
    15961 agtccgcagg cgttgccggg accgccctcg gcgggtggca tcttgggggc aatgccgagg
    16021 agcttgtcgg cgagggactt gggcgccatc agggcaatgc cgcgggccgc gccgcgcttg
    16081 aggtcgtcca ggcgcggcag caggatggcc tgcggacgtt ggaagtccgg atcgaggggg
    16141 gaggcgacgc ggaagggcag gctggggcgg ccccacacgg tgcgctcgca gccgcagtca
    16201 tcctcccagc ggacgaaggg tacggcatgg aagaggtcgt cctcaccctg ggggtagcgc
    16261 ggaaaggcga tgccgtcgtc gttgcgggcc atggcacggc gcccccgggc cggcacgagt
    16321 tgacgcagca gggcggcctg ggtctgggag agatagacat ccagaacgac ggggacgctg
    16381 agcggcttgc cggccgcgtc gaggccgctc tgactgggga gcggggcgct cgtgagggtc
    16441 gcactgccgg cggcaaggcg ggtgagccag tccagcaccg cggcttcgct gcggtcgagc
    16501 cattcgagca gggaggtgcc gtgggtcgag ggggctggca cggcgaagcg gtcggacggg
    16561 gccgggtaga aatggatctg gccgaggagg gcgcgcaggg cctcgttgtc gggctgggtg
    16621 gcgtcggtga tgcgatagcg caggaggacg ctgaggagtt cgcggaagcc ctggctggca
    16681 tagccgagct gaaccgggcg ggaatcctgc gcctgccagg cgcgggcgcc ccggctgccg
    16741 cagggccacg cgatctcctg accgagggcc gtgtccgtgt cagtcgggat ctccacgccg
    16801 ccctgctcgc gataggtgcc gccgaggggc agatagcccc acaggagggt gcggttgcgc
    16861 acgtggccgg atgcgtcgcg ggccttgacg agggccgggt ggagggggtg ggtctcttcg
    16921 ccggaatagg gcggttcggg gaagcgcggc ttgagcaggc cgtgatgaag cagacgccgg
    16981 tactcatccg gttcgccgcc gtcgccggcc ccgccttgcc agccgagggc ctcgccttcg
    17041 cggatcatcc agcgctggac ggcgccgccg gacaggccgc ggcgcacgac gaagccggcg
    17101 gagcggacct tgaccggatc gaaggccggc aggccgaagg tgtcgcagct cacttcgcag
    17161 cacgcgatgt agaaggcccg gtgcatgggc aggcgcagca ggggcgcgtc cttgtaacgc
    17221 ccgacccggt cggactggta ccagccctga ctgcgggtgg ccgtgagacg gccggcctgg
    17281 gcgtcctgca ggaagcgcgg tacgaactgg tcgtccaggt agcggtgcag gctcatgccc
    17341 tcgtccgcgc cccctgcgaa ccaggtcggc agcaggcgcg gcgtgtgatg gacggaaggc
    17401 ggccgggccg ccacggggac gtttaccata tgttccccgc gcccggtgtg tagctggacg
    17461 acaccacact gttgctgatc agggtgtcgc actggaccac gccggagaat ttgctcatgc
    17521 ccgcgttaac ctgcacggag ccggccgaga cctcgaccga ggaggcctgc acggtgacct
    17581 ttgagccggt ggtgagggtg atgccgccgg acgccatctc gatcttttcc gcaccctttt
    17641 cgatagtgac cgtgccgctg ccctgatccg tgacggtgaa gtggtagccg gcgggcgtct
    17701 cgatgcgtac gcgctgggcc tgatcgtcca tcagcagctt gaagccggcg ggcgtctgga
    17761 cgaggtagcg ctgatccact ggcgcggcgt cgccgggctg gctgctgccg ccggcccaca
    17821 gggcgccgat caccaggggc aggtcggggc tcacgaaggc gatgacgacc tgttccccga
    17881 cccgcggcag caggctgacg ccatagccat tgccagcgct ggcgacgatg acgccggccc
    17941 aggtttccag gcccgtggcg gcgaggcgga tgcgcaggcg gccgcggttg tccgggtcgc
    18001 tgttgtccag caccgaggcc aggtggagtt cgtggagggg gccgaggctg tgggggctca
    18061 tgggggcgcc tcagctcagg tcgggccgct cgacgcgcag gtgggtgcgc aggccggcgg
    18121 aagcgtcgaa gcggtgatgg cagtccatca cccgatagcg gccggccaga cggggcgacg
    18181 cgtccaccag ttcgacctcg cgaccaccgc gcagatccgg ggtgtcgcgg cagacgatct
    18241 cgccacggac gaagcggcgg gcgctctgac gcagtgcctt gcgggccagg tcgttggcct
    18301 cgccggccgt gcgcggggcg ggatggggcc gggtggagac cgtgttccag cccaggctgc
    18361 ccagggtggc gacgccactc acgcctgccg gcgcggggac caggctggac gcgtcaccgc
    18421 tgaggtcggt ggcagcggcc agatcgtacc cccgggcttg gacgcgactg acctgatggt
    18481 tgagatcggc aatcaggcgg atcttctcgg cgttggcacc caccgtgagg cgcgccgggc
    18541 gggggtcggc ttcgtcatca cgggcccgca ggcgaccgtc ctggaggcgc aggccgatgt
    18601 cgcggggttc gagctgacgc agcaggaagg ccaggtcgct ctcgttgttc tgcagccagg
    18661 tggcttgatc gctgccggcc tgcacgtcgg cctgcaggcc ggcgtcgctg gcgatgcggc
    18721 ggatcacatc gtccaggctc tggtcttcga aagcgcggct ggtacgtcgg cgggccaggc
    18781 ggtgcagacc gtcctcggcc agcagcacga gctgcggcgc gccgtcgcca tagcgctctt
    18841 cgagggcggt gatgtcgccg ctgaaggcgg ccgtcgcgtt ggcaccttcg ccgaggaaga
    18901 gttccagacg ttgccccagc cggatgtcgt tgaagacgaa cgtggcggcg ctgccgctcc
    18961 cgctccagtt gagcacgcgc acctcggcac ccgccatgcc actgcggggc aggcgcaggt
    19021 ccaaggcgag tacggcctcg cccaggttgg gacggtcctg gccgtccacc cggaagcggg
    19081 ggcggacgtt gaggagcggg gcgtcaatcg acggcaaggc gggcctcccg ttcgcgggcg
    19141 agttcgatca ggtcgaggac ggcccgctcg gcggcatctt cagcgggctg ggccggggac
    19201 tcgtgggggt cgggggtgat ttcggccgag agttcttcga tgacgacggg catggcggct
    19261 cctagtcgcg gccggggatg cggaccccgc cggccggggt gaagcggggg ttttcgaggg
    19321 cgttgaggtc ggcgacgccg cgccagtcgc ggggatcgcc gccggcagcc tgggtggccc
    19381 ggtccgcgct gacatcgctg ccgcccgggg cgaaggcggg ctgggcgcgg gcggcggcgc
    19441 gcacggattc gtcccggtcg aactggtagc ggtcttcctt gaaggtgagg gccagcgtgg
    19501 cgcgcagcgg tatgccttca ggggcaaaga aatccagcgt ttcaccgtag gaggcaagca
    19561 tgccggtgaa tttgaagctg ccccactgga agcggcagcg cttgggcgcg ccaggacggg
    19621 cggagtcggg atcgacgggc ttcatgaagg tctcggcgat gcgtccggtg agggcccgca
    19681 cgtcggaatt ggcgggcgtg ccctgctggg cgaccgaggt gtcgaagacc agttcgacgg
    19741 ccagggagga ttcgctcttc tctacgtact gggcagcggg gcggcgcccg cgacggccgc
    19801 caccgctgtc ggcgtgcagg gtgttggcga gggtcacacg cagggtggac gggttgaact
    19861 gcaccgggat gtgcttggcc ggatcggtct cgacctggtc gccgttcatg gggatgagca
    19921 cggccttggc gaagctgggc ggagcgcctg cggggctcat ggggcgcctc ccgcttcgag
    19981 ggtaaagcct tcatgggcga ggtgcagttc ctcgatggcc acctggctgg cggtggccga
    20041 gaggtccggg cccttgaact tgaccggcag cacctggtgc agccaccact tgacggtggg
    20101 gccgtcggtg aaggcgcggg agggatggaa gacctcgatc agcccggtat aacgcagggc
    20161 gtagttggcc tggcgattca tcgcgtcgaa ccacttccac aggtcgccta cttcggtgaa
    20221 gccacgtttg aggaccaccg gcgagaaggt ggtgggcccg gagagctgca cttcgcccca
    20281 gttgcggcca ccttccttga gattcttgac cgccacgctg gcttccagcc cgctgacttc
    20341 gctgaaggca ccgcggcaca cgagctggct gccgccttcg tccgcaaaga gggcgacgcg
    20401 gaagcggaag gggatgaatt cggccggaac gggcgcgctg tcagccatcg agggcctcca
    20461 tctgcacacg ggcgtcggcg ttgtgccggt cctgcaggaa ggtgatgcgg atcaggtcca
    20521 gcggaaaggc cggtgcgatc tcgatgtcgg cgatcagcgt gctgtcgccg tcactgcgga
    20581 tggccaggcg ataggcgtct tcgggccgtg cgccgcgcag ggctccacgg gcgtgaaggt
    20641 gtgtgaagaa ggcacgcagg gccatctcgg tgcggggatc atgcacatcg gcgtcgaaga
    20701 ccaggcgctc gcccagcgcc cgcaactggc gttgcagcca ccccatgaag cgcatgacct
    20761 cggcggaata ccagtgatcc tgaccgcgcc gtgcggcgtc gagctggcgc agggccggca
    20821 gcggcaggca gggaacgcac aggcgttcat cgtccacgac gatgcgcccg ttgcgacgca
    20881 gcaatacggt gacgcccggt gcctgacgca gggcggcggc ttggttctgg tccagcaccg
    20941 gccagggcag atcgcgtccg gcgaagggac gttcgccgat ggagcgccac ggcccggcgc
    21001 gctgggcggt gagggcctgc aatccggcga cgaggccgca cggggagcca aggggacgat
    21061 cggcaccccg cagataaggg tagatccact gcaggtgacg cagtgcggag gcggcgtcga
    21121 gactggcagc gccttccttg agggcgctgc cgaccgtccg cgtgaggtag gcggtccagg
    21181 cgggggccgg caccgggact tcctggccgg cgtccgcgtc caggggcagg gcgagcagac
    21241 actgcatgtc cggtcgcagg cgggcgaggg tacggatcag cggctcgatg acggcctcgg
    21301 ggggcagcgg ttcggtggcg tggggaatct cgtcgccgcg gcgacgttcc cggtggccgt
    21361 cgtccacggc cgtgcccacg ggaaggaagg ccggcaccgg gttatccagc tgcatgcgcg
    21421 ggatgtccgg gaggagggcg ggcacctgga ggcgttcaag atctggcagc acgatcagcc
    21481 ccgcccgcgg ggggagcagc gcccgctcga aggcgccaag gcgtccgatg tcggtgagct
    21541 gggcgcccgt gtcggggaga aaaccgtgag cgccttcggc ctcggggacg cggatgaccc
    21601 acagcttgag ggcttcgcgg ccgagggagg tgctgtcctc gaagaagtcc tgtacggccc
    21661 gaggcagcca ggcccggtcg ccggccaggg tgctgaccgc cctacacggg gtgtcgaagc
    21721 tgtacgggaa gagcacgcgg aactggccaa gggcgtggat cggcagcggc agacggtaga
    21781 gggcgcgcag atccgcatcg ccgggctcgg gggtgacgga ccgtccggcg ctgtccacgc
    21841 ccgcgcgcag ggccttgagc cggcgcacct cccggctgaa gccgagggcg gcgatgtcgg
    21901 cgagcaaggc ctcgtcgtcg gccacccgct gccattgggc accggacgcg ccgctgccgt
    21961 cgtccgggcc ggggcaatgg ccgagcatga ccacctccag caccggcggc acagggggcg
    22021 ccgggcgggc gtcaatctcg atgcgctggt cggcgaggaa cacggaccga ttactcctgt
    22081 tccaccgtct ccacggacag caccagctcc tcgatggcca cgtcgccgcc gcccttggcg
    22141 gtgagggtgg gtccggtcca tttgataggc atggcgccgc gcagcttcca gctgacgacg
    22201 gtgtcggccc ggtcttcgga gaggagcttg atgacgatct cgcgcttggc gtccttgatg
    22261 cgcccgctgc ggctggcttc gagccattcg tagaggttct gggcgccgat cacgccgcgc
    22321 ttgagggtca cgtcgcccgc cttgtggatg ccgggcagct tgtgcacgta attgaccgtc
    22381 gaattgccgg cgcggtattc ggcgacggtg acttcggcgt tgaggccgga gacctcggag
    22441 aagccggcct ggacctcctt gccctggccg ggctggagct ccacgaggaa gttgaaggcg
    22501 gcgtaggggg tttcgcgcat ttgggccatg gttcagctct ccaggtcagg cgtcggcggt
    22561 cttctggccg atgcggaaga tcacgaattc ggcgggacgc aacggggcga cgccgaccac
    22621 gcagacgagc cgcccgttgt ccaggtcgtt ctgggtcatg gtggagcggt cacaacggac
    22681 gaaataggct tccttggggg cgccgccgag gagccggccg ttcttccatt cgttgaacag
    22741 gaagtcctcg accgagctgc ggatgttggc ccacagggct tcaccgttgg gctcgaagac
    22801 cgcccattgg gtggattttt cgatggagcg ttccaggtag aggaagtagc gccgcacatt
    22861 gacgtatttc cattccggat cgctggacag ggtgcgtccg ccccacacgc gcaggccgcg
    22921 gccggggaag gcgcgcaggc agttgatgcc gttggggttg aggagctcct gctggaactg
    22981 attgatgtcc tgctcgaagc tcagggcgcc cagcacggtc tcgttggcgg gggccttgtg
    23041 gacgccccgg tccacgtcgg tgcgcgcgta gacgccggca atgaatcctg cgggcggtac
    23101 ggtgatctcg cgccgctggc cggtgggatc ggcgatggtg acccaggggt ggtaaagggc
    23161 caggcggctg tcgtcgaagt cgttgcggaa ggtgcgcact tcgcccaggc tcatgccctc
    23221 gcggctgtcc accagaccga cccggtagcg catgcgccgg cagtgcttga gcatctccat
    23281 gagcacggcg gtgtgggtgt cggcgtgggc ggcggcggcc gggaccatga cgatggagac
    23341 gtcctcgacc gcttcgaggg cggcgaggcc ggtgctgccg aggagttcat tggtctctcc
    23401 ggcatagtcg ccggcacccg gcacattgcc gtcggtcccg ccggacaggg tgatgaggta
    23461 gcgcggatcc ttgtagctgc cggccggggg aaacagggcc gccggggtga acagggtgta
    23521 gagcgtggcc gcgagcgccg cgccgtcgag ggtcttgacg gtgcatgcca ccggcgagtg
    23581 gagggcgtcc tccttgcgtt ccggtgccgc aggcagggcc ttggcgaggc tccggtcgcc
    23641 cgaggcggtg aagtccaggc tgccgtagac gtagaggatc tcgccgtccg ggccgccgtt
    23701 gcgcacttcc agatcgaagc tgcgctggac caccagggcg ccggccgcgc ccggtacgct
    23761 ggcgagggcg gcgaggggca cgctgacgtc cttcgtgatg cccgggttga gggtcgcgtc
    23821 gcccttcacg gtgaaggtct ggccgccctt gtcgagggtg ccacgcagga ccagggcttt
    23881 accccagtgg tcgcccttgg tgaacgccga caggtcggct tcggccttga ggatgaggac
    23941 ggccagcgcg tcggcgctca gggcaccggc ggagggctgg cgggcataca cgcgcctgag
    24001 ctgggtgccg gcggggagct tggtggggtc gagcaccgtg atctggttgg cgccgacggg
    24061 cgccgggttg gcggggtcgg ccttggagac aagggtggcg cgggcgtcgg gaatgcgcag
    24121 gttggcgccg tccttctgga cgatggcctg gatgtccacc gggaacttgt cgctggtgat
    24181 ggcgccggtg accttggcgg cctcaacgac gccgcgggca tcgaggtagt actggacacc
    24241 gtcggctgca ctggcgacgg tctcggaggc aagcaggttt tcggtgtcgc gccacaggat
    24301 ctcgagggtc aggttgccct gagcgccggg gaatcgggat ttgaatgcga gattgccctt
    24361 gctgtcggcc accgaagcgg cggtggcgct gctgccggtg ctggtcgcgt cgctgtcgtt
    24421 gccgccggcg gccacacgta ccacgaagag ctgcttgcca ccgttgtcga aaaaggcgcg
    24481 ggcggcgtgg gacgtgaggt tgagggtggc actgccaccg agtttcagat cctcggcgtc
    24541 gccgaaggtg cgttcgaact cggcatagct ggtgagcgct tcgggtgtgc cgcgaagcgg
    24601 accactgcgc gtgggaccga caagggcggc cacgctggtc ccgacccctt cgatggattt
    24661 ctggcgaaaa ctcgtctcct cgatgaagac gcctggggcg agatactcgg gcatggtcgg
    24721 tctccttgcg ggttagttcg acttcaggcg cagcaggctg tgttccggac tgaccactcg
    24781 gctgatggcg cccggcgtga aggtgaagga cagggacggg gcaaactccg gctgaccgcc
    24841 ggcgtccttg ccggtggcga cgtcgcaggc cacgagctca tccggatcga agggggtgtt
    24901 cggatcggtc gttgcggatg ccagcacggt gagggtggcg gggtagctct tgaccaccgc
    24961 gtccacggcg accgcaggca ggcggtcgag gggaaggatg aattcgccgt gggcatcggc
    25021 ctgggccgtg taggtctggg tcgcgccgag gggaggcttg acgctgagct cgaccagggc
    25081 ccacgcggcc ggcgcgtcgt cggcaaaggt gaggcgcccg aacacgccgc cggcgctgcc
    25141 atagcggctg ccgagcgggg agcggaacaa cctgatctgg tgcccggcgg ccgtgccgcc
    25201 gttcaggctg aagcggcggg gcaggaattc gccggccgga tcgcggacaa tgccttcgat
    25261 ctggtaggga ttctgggccg gcggcacgcg ctgggcgggt gtcgcctcac cgggcacgac
    25321 gagggccagt cgggcaggct ccttggccgg tggaagcggt gtgtcggtgg tgcggaggag
    25381 ggccgtggcg cggggcttgt gggcgatccg caggttgcgg ggcgccttgg tgagctcgac
    25441 gcgcaggcgc gccggggcga tccgcctggc ggtgccgccg gcattgccgg aggcatccag
    25501 ccattcgatg aagtcgctgc cgtggacgat gacggtttcg aggatcatgg cgcgtcctcg
    25561 ggcagcgtgc cggcggggat cagtacgctg cgcaccagcg ggccctcgtc ggcgctcaga
    25621 tcgggcgcca ggcgcagggt cttgatgagg tagggggtgg acagccggta gtcaccgggc
    25681 agactgtccc agaggcgcat gagatcctcg gtgctcatct cttcgggcat cacctgcacg
    25741 ctgtcatggt cgtcccaggt ggcgtcggcg ctggccaggt gggagacatc cagggtgagg
    25801 gcgccgccga tgatctgcat ggccgcggcc aggtagccga tttcggtctc gttcttgacg
    25861 ctccagccaa tcagcaggac gtgcagattg acgggcagtt ccgggcgggg cgtgcgctgg
    25921 ggcggggcgg gggcgaggta gcggttgcgg gcaaaggggt cgatggcgat gcggtgcagg
    25981 tagatgccca ggttttcacc cgaggggccc gctgcgaggg tctgggagcc aaggacaagg
    26041 gccttcgggc tgccaccggt caggggcggc atgcgcacat caagacgctc cttgagcgcc
    26101 tgcaggactc cgaggaccgc gcgatgaccc gccatgacct actccgcctg cctcatggtg
    26161 attgctccgc aaacacgcac tgccgattgg aggacgccgg aacgctgtga ggggaccaca
    26221 gccttcctcg caccgctacg atgccatgga cgtaggaagg tgactgttac ttttttatgt
    26281 cgtattcagc cgttcttcaa ggtcgtttcg atttcggtgc tcacctgggt catcagcttg
    26341 tgtaccgggc acctggctgc gaccgccagc agctgggccc gctgggcctc atcgagcgga
    26401 ccgccgagcg tcagcctcgt gctcaaccgg taatggccgt cgcgttcgtg gctggcgtcc
    26461 cgctccactt cgatttcgac agcgtcgagc gggatgccct tgcgtcgcgc gtaccacacg
    26521 agggtcaggg ccttgcaggc gccgagggcg gcgtcgtaca ggtcatgggg attcgggcca
    26581 gcatcttcgc caccaacgtc gggcggttcg tccacggtca ggcggtgacg gccgacggag
    26641 acgatatggc gcatgggacc tgaagtgtcg cgggtgacgg tgatggtcat agcgtaggtt
    26701 tcccggaagg acggagggtg aggcgcgacc ggacgactca gaaccgcccg gcgcggaaat
    26761 cctcaagggt ctgacggatt tcctgttcgg tgttcatgac gaatggtccg tactggacga
    26821 tgggttcgcc aaggggccgg cccgcgacga ggatgagccg ggtcggcgcc tttgcagtca
    26881 gcaacacacc gtctgcatcg gggttggttt cgaggatcgc catgcgcccg gctgcgacct
    26941 ccgtatctgc cgcgccacag cagacctgtc cgcgatacac gcagacaaag gcgttatggc
    27001 cggcgggcag cggctgcgcg aagtggctcc cggcggtcat ccggatgtcc aggtagagcg
    27061 gctcggtgac gggacgttcc accgcgccgt ggatgccgtg gctgcgccct gcgatgatct
    27121 gcacgcggac accgtccggc gtggtgtaga cgggcaggtc ggccgcggca atgtcgcggt
    27181 accacggcgg acacagcttg tcccgggccg ccaggttgag ccagagctga aagccttcca
    27241 tgcgcccgtc ctgctgttcg ggcaattcgg aatggatcac gccgcgcccg gccaccatcc
    27301 actggacacc gccgcttgtc agcacgcctt cgtgcccgac gctgtcccga tggcgcatgc
    27361 gtccttcaat catgaacgtg atcgtttcga agccccggtg gggatgatcg gggaagcccg
    27421 ccaggtaatc gtccggactg tcgctgccaa aggcgtcgag catcaggaaa ggatccaggc
    27481 gtcgctgcag ggcaggagtg atgaggcggt tcagcgtgac gccggcacca tcccgggtgg
    27541 gttgtccggt gatcagatgc tccacggtgc gggggtggag gatgccttca cgctcgatcg
    27601 ccgcgatggc gcggggaggg gccgggaaat tcatgaatat gtggctccgt gaaaaatacc
    27661 gtattaaatc tgacgttata cggtcgtata atatttggaa accgtgcccc ctggattcag
    27721 actagaccat ccggggaaaa atgggggaaa cacacatggc ccgcagcagc agtgtcagtt
    27781 tcgaagcggt ggagcagatc tgctttcagc ttctgtccca gggcgagaac ccgtccttca
    27841 acctcgtcta cgccgagctg ggcaacaagg gatccagcga gatcgtccag cggtatatcg
    27901 accagtggcg gcgggaaacc ggtgcggcct tcttccgtca gcgcagcgtt ccaggcatgc
    27961 ccgacggact ggtgacggcc accgaccgct tcatggcaga ggtctggcag atgtccctgc
    28021 atcacgccga acagctgctg gaatcccgca gggtggaact cgagcaggag cgtgaggcga
    28081 tccgtgccca gttgcagctg gcgcatgacc agatcggtgc ccagaacgcc gaagccgttc
    28141 gcgtggccca gcaactggag acgctggagc gcaaggcgga agcgtgcgag atcaccatcg
    28201 gcgatctacg tgcgcagaac ggtgacctga tgcggcaacg cgaagccctc gagacccgcg
    28261 tgcgcctcct cgaagaagcc gccgagacgc gggagcagaa attcaccgcg gaatccgaac
    28321 ggctcgccaa gctcctccag accgaccggg accgttacaa cgcgtcgctc aaggaagagc
    28381 aggagcgagg tgccgtggag cgcgagcacc tgatgaaaca gattgatgtg gcccgccagg
    28441 agaaactggc ggagatcacc tccatgcggc aacagctcgc cacccgcgag gagcgtgaga
    28501 cgcgtcagcg cgagcaggct gaagaggccc gtgcagcgct gcgcgaggcc cagtcccgcg
    28561 cccgtcagat ggagacttcc ctggccgctg cggaaggacg catcgagcgc ttgcaggccc
    28621 aattgaagac tgccagcgac gcagcccatg gcaaggaatc ggccgtggcc gcggcggaag
    28681 cgcgcgcccg cgcgatggag gacgtgcgaa tcgccgaaac ggcccgtgcc cagcgcgccg
    28741 aggccgcgct ggccgagacg ctggcggaac tgggcatcct gcgcaagaag atggctgccg
    28801 gcggcgcctg agcgcccgct tgacggctaa acggcaggaa tgaatacctg gccgtccatc
    28861 agacgtcgtg cgctgcgcga catgctcacc tgacggatga cccagttgcc ggccgcgtcg
    28921 atctgtgcgt cggcccctgc cgtcagcgtg cccgagggat ggccgaagca cacgcgcccc
    28981 ccggcattga cgcgcgccac ctcatggacc agcgtgcccg ggatcgccgc cgccacggca
    29041 atggcaaccg cgcctgttcc tgtcatggcg tgatgcagcg gccccatcga gaaaatccgt
    29101 acgagcagat ccacctcgcc ggcgacgatc tgacggccgt ccgatgccac gtagggcgcg
    29161 ggtgccgcga cataggccag cttgggcgtg tgcggtcgat tgcgggtggc ctcatcagcg
    29221 gtcggggcga ggcccatggc gaccgccgcg gcggcgcgga gggcctccgc gcgggccagc
    29281 agctcgggct gcccgttcac gtcccgctga agttccgtgc ccgtcagtcc gagcgtgtcc
    29341 gcgtgcacga agaccgtcgg attgcctgca ttgatgaggg tggcatccac gtttccgagg
    29401 ccggctaccg acagcgtgtc ccgtcgatgg ccggtgggga acagggcctc gccatcgccg
    29461 gccggatcga ggaagtcgag ccggatctcg gcgcctggaa atgccacgcc gtcgagctgg
    29521 aaatctccgt cttcgaccac tgcgccatcg gccaccggga tgtgggcaac gatcaccttg
    29581 cggatgtctg cctgccagat gcgcacgacc gccacgccgt cccggggaac gcgaaccagg
    29641 ccccggtgca gggcaaaagg cgccacggcg gcactcagat tgccgcagtt gccggaccag
    29701 tcgatgacgg cccgatccac cgccacggcg ccgaagcggt agttcacgtc gcaccccggg
    29761 tgagtggagg gcccgacaag gacaaccttg ctggtgcttg aggtggctcc gccgaggccg
    29821 tccatgtgct ggcgataggg gtcggggctg ccgaccgcac ggatcagcag gcggtcacgc
    29881 aggtctggat catcgggcag atcctgctcg aggaagaaca ggcccttgct ggtgccgcca
    29941 cgcatcaggc aggcagggat acggcaggga cggacaggcg gcgggaaacg agtcactgtg
    30001 gaatctgctg ctggcgaagg tgtggtgggg ttcatcgcgg gacggatcgc accgtacgga
    30061 tggcgtgatt aaagttctga aaacagtaat tttcagaaag aaagattcgc gtgcagcgca
    30121 ggctcattgt gggtcgcacg gccgtgtgcc atgtgcgcga agccaagatc caggctggcc
    30181 agcggtgctg catcaggcac gccgtgcaag attgtcggcc agcatgttgc agaacagtcg
    30241 gccctgttcc agcgcatcgt ccagggcgac gtgggtgtgg ggtgacgggt cgaaccagcg
    30301 gtcgggcatg gcgtgctttc cgctgccgcg ataggcgcgc cggagcatgg ccatggcgta
    30361 ggtcttgatg tcgatggccg agtagccgaa aggattttcg cccgtaaaac gcatcaggta
    30421 gtaatacacg aagggaaagt cgaacgcgct tggataggcg acgaagaccg gtcggccggg
    30481 caaggccttg agccagtcca gataccgcgt catcgcacga acaggcgctt cgggatcctg
    30541 ccggcacgcg gcccaggcct ctggctgggt cttccaccag gctgtggtga cggggtccgc
    30601 gacgcagccg ggtatggtct ccaggttggc ggaaaacgtg gtgagcagtc gtttgtcggc
    30661 ggtgaacgct gccgaaccga ggctcagcat cgaatggatg ccggccaccg ggccgtcagt
    30721 ctccacgtcg gtgctcacat agatttcagc catcgcatgt cctcagcgtg tgccctgcga
    30781 tccatcgccg tccggcaacc agcgcagcag cattccgaac agggcgtccg gctcgaccgg
    30841 cttgggcagg aaatcattca tgcccacggc gaggcaatgg cggcggtctt cggcaaaggc
    30901 gttggcggtc atggcgatga tgggcgtttg ctgcatggcg ggctgcgcgc gcagggctcg
    30961 ggtggcggcg aggccgtcca tgacgggcat ctgcatgtcc atcaggatca gttcgtaagg
    31021 cgcggcgccc gccatggcca cggcccgggc gccattttcc gccacatcca cgacgagtcc
    31081 ggtatcggcg agcaggctga gggccacctc ctggttgatc gcgtcatctt cgaccagcag
    31141 gatgcgggcg ccggcgtggc gagcccgcag ctgtacttcc gcgtcctcgg gcgtacgcgt
    31201 ccggagcgcc tcggcgacgg ggcggcctcg tttcagacgc gcggtaaacc agaacaggct
    31261 cccctgcccc ggcgtactgt ccacgccggt ttcgccgccc atgagaccgg ccaggcgatg
    31321 ggtgatggcc agcccgaggc cggtgccgcc aaagcgtcgc gtcgtggacg agtcgacctg
    31381 ggagaaggcc tggaacagct ggccgagctt ttccggcgcg atgcccacgc cgctgtcccg
    31441 cacctcgaag cgcacgagca gcgcgtcccc ctcctccacc agcacatgcg cggccagatg
    31501 gatcgagccc cgggccgtga atttgatcgc gttgctggcg tagttgagca gcgcctgtcg
    31561 cagccgcgtc ggatcgcccc gcagatacgt gggcagattg ccgtagtcga tggtgacctc
    31621 caggcccttg gtctgggcca gctcgccgat gagggagcgt acgccgtcga gcagtgcgct
    31681 ggcggggaaa tccgtctcct ccaggtggag cttgtcggct tcgatctttg acagatcgag
    31741 cacatcgttg atgatggaca gcaggtgttc accggccgcg ccgatcttgt ccacctgctc
    31801 ggtctgctcc gccgacagcg ggctgcgctg caacagccgc gtaaagccga ggatggcgtt
    31861 catcggcgtg cggatctcgt ggctcatgtt ggccaggaag gcgctcttgg cctggttggc
    31921 cgccaccgcg gcggatttgg cctgatgcag cgccgcttcc gcctccttgc gccgggtgat
    31981 ctcggtcagg aagacgatga agcggcctcg ctcgccgcct tcccgaccgg caaggtggta
    32041 gatggtcaac tccaccggaa ccgggtggcc gtccttgtgg cgctctgcgg tttcgatctg
    32101 gatccgccct tccctgcgga tgcgctcggc aatcgcccgg taggcatcgg gtgggaaatg
    32161 gggatccaca tccggcaccg acaactgcaa catttcctgc tcggtgtagc ccagcagacc
    32221 ggccgagaag cgattgacat aggtgaagcg cccgctgtcc gcatccaccc agtggatgcc
    32281 gatcccgacg ctgtccatgg caaactgggt gtcgagcagc ttggccgtgg tggcccgcag
    32341 ggccgaggtg cgttcggcga cgagttcttc caggtggtgc cggtagcgct ccagttcggc
    32401 cccgaggcgc cttttttcgg tgatgtcttc cttgatcgcc acaaaatggc tcaccgtgcc
    32461 gtcgggctgg cggatcggcg tgatcagcgc cagctcaacg tactcggtcc cgtcacagtg
    32521 ccggctgacg aattcccctt tccaggcctg tcctgccgcc atcgccgtgc gcagggcggc
    32581 gtaggtggtg tccggcgtca ggccggattt caggatgcga gcgtgctggc cgacggtcgt
    32641 gtcccgcgta cgaccggaac tgtgcaggaa agcgtcgttc acgtattcga tgcggcccgc
    32701 cgcgttggtg atcacgatgc tgtccgggct ctgctccacc acctgggaca gtttgcgcag
    32761 cgccgcctcc gcctccagac ggcgggtcat gtcgtgggcg atcacggtcg ccgcgcggtg
    32821 gccgtcgaat tccagctccc ggaccgacat ctccacctga atgcggctgc cgtccttgcg
    32881 aacgtgggtc caga
//
LOCUS       DAOQPA010000029        31597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_632_length_31597_cov_18.271987, whole genome shotgun sequence.
ACCESSION   DAOQPA010000029 DAOQPA010000000
VERSION     DAOQPA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 31597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_31597_cov_18.271987"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKX01_06090"
     CDS             complement(<1..680)
                     /locus_tag="PKX01_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMZ75730.1"
                     /translation="MTILVVAEHDNAALKSATLNTVTAAARLGGEVHLLVAGAGVGGV
                     AESAAKLAGLAKVLVADAAHLGEQSAENVAAQILAVVQAGGYSHVLAPATTFGKNVLP
                     RVAALLDVAQISDIVGIEASDTFVRPIYAGNALATVKSADAVKVITVRTTAFEAAGEG
                     GGAAVAAVDAVADSGQSKLVSRELTKSARPELGAAKIIVSGGRGVGSGENYHRLLEPL
                     ADKLGAALG"
     gene            complement(680..1429)
                     /locus_tag="PKX01_06095"
     CDS             complement(680..1429)
                     /locus_tag="PKX01_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMZ75731.1"
                     /translation="MKILVPVKRVVDYNVKVRVKSDGSGVDVANVKMSMNPFDEIAVE
                     EAVRLKEAGVATEVVAISCGVGACQETLRTAMAIGADRGILVDTDVELQPLAVAKLLK
                     ALVDKEQPSLVVLGKQAIDDDSNQTGQMLAALLGWPQATFASKVAVGAGKATVTREID
                     GGLETLEIALPAVVTTDLRLNEPRYATLPNIMKAKKKPLETVKPADLGVDVTPRLTTL
                     KVAEPAKRSAGVVVADVGQLVDKLKNEAKVL"
     gene            1640..2224
                     /locus_tag="PKX01_06100"
     CDS             1640..2224
                     /locus_tag="PKX01_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ75732.1"
                     /translation="MEKTTRPQLDRAAWIQAALEVLADEGVQGMRVEVLAKRLRVTKG
                     SFYWHFKDRQDLLSGVLETWKAGRIQDIVKQTRLEPGRERAQIARLVDIYSTSRSRKG
                     LLIELAVREWARRDAEAAAIVEEVDAWRLRCARELFLACGLPQHEASTRSMLLYAYVF
                     GASIMIYDRFDADIPRLTEDIRALIVGPPAPTLA"
     gene            complement(2234..2998)
                     /locus_tag="PKX01_06105"
     CDS             complement(2234..2998)
                     /locus_tag="PKX01_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ75733.1"
                     /translation="MLIDTHCHLDAAEFADDRSAQIDAARAAGVGRFLVPAVDAGNLD
                     AVAALSAAHADCRHAFGIHPMYVARAQADDLERIRSRLADGSAVAVGEIGLDAYVPDG
                     DLPRQTEFFEAQLRLARTFDLPVVLHIRRAQDAVLGALRRIRVRGGIAHAFNGSRQQA
                     EAFIGLGFKLGFGGAMTYSGSRRIRELAATLPLDALVLETDAPDIPPAWAARQRNLPA
                     NLRRIAAELAALRGMDEAELIAATGRNAVAVIAGLR"
     gene            complement(3009..3809)
                     /locus_tag="PKX01_06110"
     CDS             complement(3009..3809)
                     /locus_tag="PKX01_06110"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HMZ75734.1"
                     /translation="MSAVVDTPQEPMLLRTDADGVTTLTLNRPGQFNALSQAMLEALL
                     AELERIAEDSAVRVVVLAGAGKAFCAGHDLKEMRGNYTKSFQQALFRLCGKVMMKIVA
                     MPQPVVARIHGIATAAGCQLVSMCDLAVASDVARFAVSGINVGLFCATPSVGLARNMG
                     RKEAFEMLVTGDFIDAQEARRRGLVNRVVPLEQLDDEVAKLTASICAKSPAAIAMGKR
                     MFYAQLEMGLDAAYQMAAEAMACNMMCEDAAEGIDAFMAKRTPVWTGR"
     gene            complement(3878..4507)
                     /locus_tag="PKX01_06115"
     CDS             complement(3878..4507)
                     /locus_tag="PKX01_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HMZ75735.1"
                     /translation="MTHSLRACLSASVSFVVALVSLAGCGAVLPKGAPTFAVHDFGPL
                     APVGPRSLPYPIRTLEVIPAPWLASTAMQYRLAYAQETRRQAFVQSRWAAQPGQLLEV
                     ALKRSVRVNVTTVSLGGCRLRVDLDEFMQVFDNESGSRGVIDARASLLAPRTDQLLAT
                     QGFSVTRPASSPDAAGGVVALRDAVQAFDAALLTWLDGLAGSVGARCSQ"
     gene            complement(4515..5459)
                     /locus_tag="PKX01_06120"
     CDS             complement(4515..5459)
                     /locus_tag="PKX01_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ75736.1"
                     /translation="MEDRAHAIAAGLFALLLGTALVFTLWWFSDKREVTRDVVLVSAG
                     SVNGLNPQATVRYRGIMAGKVASIALDPARPQDLLVTARIRADLPLTHGTRARLATQG
                     VTGIAFIALDDSGADPTPLAADGNGPPRIALEPGLVEEVADATRRTLGQLRELSERFA
                     RLASNENIGRVERILAHMESSSKGLDKGMQDAAASLAAVHRMLGKDNLARIERTLANA
                     ERFSSDAAPLAADGRRLLARMQDVADRLDVIAASTGEGFATSTLPRLNVLLQELTTTS
                     RQMNDLLDELDSHPQIFLLGRGKAPPGPGEAGFVPNRP"
     gene            complement(5459..6235)
                     /locus_tag="PKX01_06125"
     CDS             complement(5459..6235)
                     /locus_tag="PKX01_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ75737.1"
                     /translation="MSAFPVEVKGLSTRFGDTWVHRGIDLAVPAGEIVALVGGSGSGK
                     TTLLRQIVGLLRPTAGEVRLFGEPLFGGSRLEQVARRRRFGMLFQQGALFSALDVYAN
                     IAFPLQEAGVRDEAMLHDLVGVKLAMVELAPEVGLRMPAELSGGMVKRVALARALALE
                     PELLLLDEPTAGLDPDRSAGFVRLIRDLHRALGLTVVLVTHDLDTLAALATRVAVLAE
                     GRILGFGSLDSVMALEHPFIERFFRGERSRAALARGRAEA"
     gene            complement(6232..7362)
                     /locus_tag="PKX01_06130"
     CDS             complement(6232..7362)
                     /locus_tag="PKX01_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ75738.1"
                     /translation="MNAAPQAAPALRVDGHGAAATVTLTGDWTLRALLPRLSVVQAAL
                     AEVHGVAVWDLDGVIRLDTFGATLLWRHWGRQRPARLQVPTILDENFVRLAALGGPSV
                     PVAPPYRFLDATAHLGRLAIGFGRHLADFTALVGQILLDALHLLGDRQRWPWREMSAN
                     FYKVGVKAMPVTALVGFLIGVVLSYLSALQLRAFGADIYIVNILGLGIIRELGPVLVA
                     VLVAGRSGSAMTAQLGVMRVTEEIDALATMGISRYVRLVLPKVVALFLAMPLLTLWCS
                     AVAIVGGMVSAWLQLGIGFEYFLQALPRAVPFPNLVIGLSKGAAFGALIALVACHFGL
                     RVKPNTESLAAHTTASVVTAITLVILTDAVFAIATRSIGVPG"
     gene            complement(7388..8890)
                     /gene="ppx"
                     /locus_tag="PKX01_06135"
     CDS             complement(7388..8890)
                     /gene="ppx"
                     /locus_tag="PKX01_06135"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HMZ75739.1"
                     /translation="MNHELIAAIDLGSNSFRLQVGRIVNDQIYPLDGIKESVRLAAGL
                     SPDRMLDAAAQFRGLNALRCFNERIRDYPPEAVRAVATNTLRVAKNAPHFLIQAEAAL
                     GFPIEVIAGREEARLIYVGVAHTLPNPHRQQLVVDIGGGSTEFIIGKSFKPLELESLY
                     MGCVGYSLRYFPDGRVDKKGLREAELAALKEIQTISHAYRRTGWEEAVGSSGSAKALV
                     EILELNGLSAGGITRDGLEKLKVLMLKAGSVQALQLEGLRPDRLPVLPGGFAIMSAIF
                     QEFGLERMVFSEGALRLGVLYDLLGRYHHHDLRDATVTAFMERYSVDVAHARRVADTA
                     LHFLEQLDPSTEDPENTDRRFVEWAALLHEVGVSVAHSSYHKHSAYILGNADMPGFSR
                     MDQGRLSRMVLAHRGKLARVSALDPESLEWRFILCLRLAAVVHRARDGRGVPPLRVKR
                     EGRAYVLLADGDWLRNLPLTAAALDEEERQWQGIGMPLRVRVERAARGAA"
     gene            9041..11125
                     /gene="ppk1"
                     /locus_tag="PKX01_06140"
     CDS             9041..11125
                     /gene="ppk1"
                     /locus_tag="PKX01_06140"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMZ75740.1"
                     /translation="MRPAPAPRYSTDHFINRELSLLQFQRRVLAQAADPAVPLLERLR
                     FLCIVSSNLDEFFEIRVAGVKEQMRLGRVATTADGTSPRVLYEHISREVHSIISEQYA
                     LLNDTILPALAAEGIVFLRRVAWTEAQHTWARDYFLREVMPVLTPIGLDPAHPFPRVL
                     NKSLNFAVELEGRDAFGRNSGAAIVQAPRALPRVIRVPRELADAEYTYVFLSSLLHAH
                     VGTLFEGMNVQGVYQFRVTRNSDLYVDEEEVKDLRATLKGELQQRHFGDGVRLEVADN
                     CSSAMADFLKSQFGLGDADVYRVPGIVNPVRLIQVPDWVDRPDLKYRPFLPARPKGLD
                     KRDNIFAAIRRQDILLHHPFQSFEPVIELLQAAADDPQVMAVKMTVYRTGTDSVLMEH
                     LVRAAQKGKEVTVVLELMARFDEEANISWAARLEEAGAHVVYGVFGYKTHAKMLLLVR
                     REDRGLKRYVHLGTGNYHPRTTRFYTDFGLLTANEEIGADVAEVFKQLTGLGQAGKLR
                     HLWQAPFTLQPNVVAAIRNEAEIARAGRKARIVVKVNALLEAETIEALYDASCAGVDI
                     TVIARSVCALKPGVPGLSENIRVRSVVGRFLEHHRIFYFHADGAEKLYLSSADWMERN
                     FFKRIEVAFPILDPRVKRRVVKEGLRPYLGDNCQAWELDQDNHYRRKNPRTSRRAAQE
                     LLLKDVAGVTLP"
     gene            11230..12483
                     /locus_tag="PKX01_06145"
     CDS             11230..12483
                     /locus_tag="PKX01_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75741.1"
                     /translation="MSLRPAFLSRRRLVVGALLGSVILAGCGGKGSSANAPTGGITVT
                     PGDTQVTITWQQDAGVEYWLWYAPGSTINPSDSNTPGIRYHINIGSPYVVTGLTNGTQ
                     YAFALNGRINGGKGGDYAYGTTTPRLAGTTWTAVTNLTADMRGLAYGTTSTVTTATYV
                     AAGTAGAAFSSTDGHTWTALANPAGTANVNAMTVGLSTFIAVGDGGAIYYSTDLSTWT
                     QATSNTTNKLTAVATSGGVAVAVGENGTIRYSTSAGTWTAAASVPSGIGHLYGISYTP
                     SGLWIAVGAGGVVLTSSDGTNWTQRTSGTAATLYGVSGMATTSGSTTTYTYVATGAGG
                     QVLTSSDGTTWTASTVSPAVTLYSVIGSNQFVAVGANGAAFTSPDGVTWTYYAASSGN
                     DLYTLMKAANQYIAVGANKAMAYSQ"
     gene            12577..13902
                     /gene="bioA"
                     /locus_tag="PKX01_06150"
     CDS             12577..13902
                     /gene="bioA"
                     /locus_tag="PKX01_06150"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HMZ75742.1"
                     /translation="MSANAALLARSLAAVWHPCTQMKHHEQLPLIPIARGEGPWLYDM
                     DGRRYLDGISSWWVNLFGHANPRIGHAIKDQLDRLEHVLLAGFTHEPVVELSERLAAL
                     TGHRLGHAFYASDGASATEIALKMSAHFWRNQGQPHKNRFVCLAGSYHGETVGALAVT
                     DVALFRDAYAPLIRPASCVPNPDARQAMPGETAVDVARRAAAALETFLAEHAGETAAL
                     IVEPLVQCASGMAMYDPEYLRLARALCDRHQVHLIADEIAVGCGRSGSFFACEQAGIT
                     PDFLCLSKGISGGYLPLSIVLTRDAVYNVFYDNDTARGFLHSHSYTGNPLACRAALAT
                     LDIFAEDEVIAANRHRATRLGEAFRAAVGDHPRVRHLRQQGMILAFDVADAPPDFARR
                     YFAEALQRELLLRPIGPTVYTLPPYILDDVHIDFLARRSAEALEAALAG"
     gene            13892..15076
                     /gene="bioF"
                     /locus_tag="PKX01_06155"
     CDS             13892..15076
                     /gene="bioF"
                     /locus_tag="PKX01_06155"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HMZ75743.1"
                     /translation="MPAEAHDPLDALATGLAELDAAGLRRRRRVVASPCAPAATLADG
                     SARIAFCSNDYLGLASDPALIDAARAGAARWGVGSGAAHLVSGHTVAHDALEARLAAF
                     TGFDAALCFTTGYMANIGVMPALVGRGDAVFADRLNHASLVDGALLSRADLHRYPHGD
                     VDALARQLTASSAPRKLIVSDGVFSMDGDIAPLPRLLELAETHKAWLLVDDAHGFGVL
                     GPQGRGSLAHFGLRSPRLIYMGTLGKAAGVAGAFVAARADVIDWLLNKARSYIFTTGA
                     PPLLADTLLAAVDLIEQGDARRAHLSALIQRLRNGLTLRRWHLMPSTTPIQPLVIGDN
                     HEALAVARALDEAGLWVPAIRPPTVPKGSARLRITLSAAHTIEQVDRLVSTLRRLEGG
                     AP"
     gene            15073..15801
                     /locus_tag="PKX01_06160"
     CDS             15073..15801
                     /locus_tag="PKX01_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75744.1"
                     /translation="MSALVLLHGWGLSHRVWAPLLAALPGHAMLTPDLPGHGDAPRAG
                     PRLADWSDAVAAGLPKGCTLAGWSLGALLALDIARRHPDKVARLILTGASPRFTATDD
                     WPCALAADTLRDFRTAFAAEPDATQRRFVALQALGDHARRNVTQTLTAALTPADTAHR
                     EALADGLALLADTDLRPILPAIAQPVRLLHGARDALMPVAAAEHLADALPHARLSIFA
                     DSGHAPFLSRPDDFATLVEGARHD"
     gene            15794..16567
                     /locus_tag="PKX01_06165"
     CDS             15794..16567
                     /locus_tag="PKX01_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ75745.1"
                     /translation="MTDAPASKRAVRAAFDRAAAAYDSVAHVQRSACDRLFALGTPHL
                     STAARVVDAGCGTGYALPGLRQRFPDAELCAVDFAPAMLARLPAGLAHPLCADLEQLP
                     VADACVDLLWSSYALQWCAPRRALTDIARVLRPGGQVWLATLGPGTLFELRDAFAAVD
                     DAEHVLRFQPPEVVEDAAESAGLRLLASERDTLQAWAPDLRRLLHDIKTLGAHQTGAT
                     RRPLRKTDLARLTAAYEAYRTPDGLPASYDTLWLVAEKP"
     gene            16564..17229
                     /gene="bioD"
                     /locus_tag="PKX01_06170"
     CDS             16564..17229
                     /gene="bioD"
                     /locus_tag="PKX01_06170"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HMZ75746.1"
                     /translation="MSAYFITGTDTEIGKTFSTCALLHAARAQGLRALGMKPVAAGAE
                     WVNGEFLNEDAARLRAAGSFDPGLALLNPYCLASPIAPHIAAREEGVRIEPARIRTAF
                     DSLRAQADLLLVEGVGGFRVPLGDDYDSADLARDLGLPVILVVGLRLGCINHALLSAE
                     AIAARGLTFAGWIANRVDPAMLRVDENLAALQKGLPAPLLGVLPFREDRDAALAATAL
                     RLP"
     gene            complement(17246..>17995)
                     /locus_tag="PKX01_06175"
     CDS             complement(17246..>17995)
                     /locus_tag="PKX01_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ75747.1"
                     /translation="HALATSARERRKAGETLLDGAHLVQVALSRGQALRQIVVSDAGF
                     AQAEIVTLLAAAPDVAQLVLPDALFAHVSPVDSPSGVLAVMALPAGQAGNSPTGDCVL
                     LDGVQDPGNLGTILRTAAAAGIAQVLLTPGCAQAWSPRVLRAAMGAHFLLHIDEGIDA
                     SAALAGYPGAILATALQDATELFATDLRGPVAWLFGSEGQGLSEGIAALATGRVRIPM
                     PGGMESLNVGVAAGVCLFEQVRQRGLHPSGG"
     assembly_gap    17996..18095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18096..18364)
                     /locus_tag="PKX01_06180"
     CDS             complement(<18096..18364)
                     /locus_tag="PKX01_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75748.1"
                     /translation="MKVHHLLLFLHLAGVIVWMGGMVFAHLCLRPAALELPPAQRLSL
                     WRAVFGRFFPLVAAAVATILLTGFAMLADVGFAQAPRTWHVMALLG"
     gene            complement(18361..18957)
                     /gene="rnhB"
                     /locus_tag="PKX01_06185"
     CDS             complement(18361..18957)
                     /gene="rnhB"
                     /locus_tag="PKX01_06185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMZ75749.1"
                     /translation="MERLCGVDEAGRGPLCGPVVAAAVILDPARPIAGLADSKKLSAA
                     RREALARTIKAQALAWHIAEASVEEIDRLNILHASMLAMQRAVAGLNLRPTRVQVDGN
                     RCPALDLPCEAVVRGDSLVAEISAASILAKTARDALLVALDRRYPQYGLAGHKGYPTA
                     AHLAALKAHGACDIYRRSFGPVRAVVAQASLWNEDPHP"
     gene            complement(18957..20105)
                     /gene="lpxB"
                     /locus_tag="PKX01_06190"
     CDS             complement(18957..20105)
                     /gene="lpxB"
                     /locus_tag="PKX01_06190"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMZ75750.1"
                     /translation="MTVRIAMVAGEASSDLLASHLIQALRRHLPDAEFYGIGGPKMQA
                     LGFQALWPAEKLAVHGYVDALRHYRELSGIRRQLKARILADRPAAFIGVDAPDFSLWL
                     ERRLKDKGIPAIHFVSPSIWAWRRGRLKGIARAVSHMLCLFPFEPELYRTAGVPVSYV
                     GHPFADVLPLAPDRAGARELLQLSGTGPVFALLPGSRQSELRNLADTYIATAAILHER
                     HPDATFLVPLATRETRLLWDAAMARHAHLTLPLRTLFGHAVEAMTAADVVLVASGTAT
                     LEAALLKRPMVITYRIGKWSWRLMKRLAYLPWVGLPNVLAGREVVPELLQDAATPQAL
                     ADALDAWLQDAGRRGELLETFTDMHRQLRQDTASRAAEAILPYLRGRV"
     gene            complement(20109..20882)
                     /gene="lpxA"
                     /locus_tag="PKX01_06195"
     CDS             complement(20109..20882)
                     /gene="lpxA"
                     /locus_tag="PKX01_06195"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMZ75751.1"
                     /translation="MSIHPTAIVHPGARLAEGVQVGAYSIVGEHVVIGEGTSVGPHVV
                     IEGHTTIGCNNRIFQFCSLGSEPQDKKYAGEPTRLEIGDGNTIREFCSFSTGTVQDGG
                     LTRVGHDNWIMAYVHVAHDCVVGDHTIFANNATLAGHVTVGDWAILGGFTGVHQFVRV
                     GAHSFCGVGTVLLQDLPPFVTVSGNPAAPHGINSEGLRRRGYSSDGITAIKRAYKTLY
                     RSGLTLDEARRQIAEAAANVPELQMFSDFIADSGRGIVR"
     gene            complement(20879..21310)
                     /gene="fabZ"
                     /locus_tag="PKX01_06200"
     CDS             complement(20879..21310)
                     /gene="fabZ"
                     /locus_tag="PKX01_06200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HMZ75752.1"
                     /translation="MDINEIKQYLPHRYPFLLVDRIIELELDKRVVGIKNVTINEPFF
                     PGHFPTNPVMPGVLIVEAMAQTAAVLAFKSDGSVPDEHSTVYFASLDNVRFKRPVVPG
                     DQLVMEAEIVRKMRNIWKFRAVARVDGELATEAEFMCAIKR"
     gene            complement(21316..22347)
                     /gene="lpxD"
                     /locus_tag="PKX01_06205"
     CDS             complement(21316..22347)
                     /gene="lpxD"
                     /locus_tag="PKX01_06205"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ75753.1"
                     /translation="MQITLGALVERLGGELIGDPSIVIRQIATLEKAGEGDLAFLANP
                     KYRAALDGTTASAVIVDPKSAGLTERARIVTRDPYLYFARAAQWFNPLRRPPAGIHAS
                     AVVASALPESVSVGPNAVIGEGCEIGENTVIGPGCHVGDGVRIGPDGLLHAGVTIYAD
                     CVVGARAILHSGAVIGADGFGFARDSDKRWVKIPQIGRVVIGDDVEVGANTTIDRGAL
                     DDTVIGNGVKLDNLIQIAHNVHVDDDAIMAGLSGIAGSSRLGKRVMVAGQAGISGHLS
                     VGDDIVVSAGTIITKTLDKPGVYTAMLPQMTHADWVKNVSHVRRLDGLADRVRELEKL
                     LAEKNKENS"
     gene            complement(22360..22863)
                     /locus_tag="PKX01_06210"
     CDS             complement(22360..22863)
                     /locus_tag="PKX01_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ75754.1"
                     /translation="MKGIGISTLAGVLLAVFAQTAAAEVKVGFVNSDRIMKEAVPAKK
                     AQQKLEKEFEKRDQDLQRMAKQLQGMQEGLEKNGVTMAEGEHRAKEREFNDLNRDFQR
                     KQREFREDLNQRRNEELAGVLERANKTIKAIAEAEKFDIIFQEAVYASPRIDITDKVI
                     KALTETK"
     gene            complement(22893..25184)
                     /gene="bamA"
                     /locus_tag="PKX01_06215"
     CDS             complement(22893..25184)
                     /gene="bamA"
                     /locus_tag="PKX01_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HMZ75755.1"
                     /translation="MNSKLLPAVIAALICASPALAFEPFVVKDIRVEGIQRTEAGTVF
                     NYLPVKVGERFSEDKASQAIKALFATGFFKDVRIEVDGDVIVVVVDERPAIAQVDFVG
                     IKEFDKEALKKGLKEVGLAESRIFDRAVLERAEQELKRQYLSKGLYGVQITTTVTPLE
                     RNRVGVNFNVVEGDAAKIRQISIIGNKVFKEKDLLDLFALTTPGWMTWYSKSDQYSKQ
                     KLSGDIEALRAYYLNRGYLDFTVDSTQVSITPDKKDIYITLSITEGEKYTVSDVKLAG
                     DLVLPEKEVLNLVKVKSGDTFVRDNVTATTKAISDRLGNEGYAFANVNAAPEVDKVKR
                     QVAFTFYVDPGRRVYVRKINFAGNARTRDEVLRREMRQMEGAWYDGAALNRSRTRLDR
                     LGYFDEVTVETPAVPGSPDQVDANFSVKERATGNLMLGAGFSSSEKVILSGSISQQNL
                     FGTGNSMTLQLNTGRINRTAALSFTNPYWTIDGVSIGWDVYQRNVDPTSLSVASYKTH
                     SLGAGLRFGYPIAEDDRINFGLAVDRTKITVFDTSPIQYVDFVNKFGSTAESLVSTVG
                     WSRDRRDSFVYPTSGTYQRVGLEMATAPMDIRYFRSSYQHQHWIPFGGGYALMLNGDV
                     GYAHGYDGKELPFYKNFYAGGIGSVRGYQQSTLGPQFVDVYGVARALGGNRRIIGNAE
                     YYFPMPGQGKEKSMRMSMFLDAGQVWGVEEKVRLSDLRYSAGLAFSWSSPVGPLKFSL
                     GQALKKEPQDRTQKFQFQLGTVF"
     gene            complement(25191..26555)
                     /gene="rseP"
                     /locus_tag="PKX01_06220"
     CDS             complement(25191..26555)
                     /gene="rseP"
                     /locus_tag="PKX01_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMZ75756.1"
                     /translation="MILVDYVLPFLLALGILIVVHEFGHYQVARWCGVKVLRFSLGFG
                     KPLMLWRAGADRTEWAIAAFPLGGYVKMLDEREGQVPTEELHRAFNRQNVWKRIAIVA
                     AGPLANFLLAITLYWGLYGGGVEEIRPRVGTPVAGSQADLAGFQDGELVRAVSGEQVQ
                     TWQEFRWKLLNRALEHQAVEVETQNGQGEIAFRRLDLAGITVDQGTEDITAQLGMKLF
                     RPVLPAVIGSVAPDSVAARAGLQGGDRVLRIDDKDIGGWSELVMMVRDAPGRRLRFEV
                     VRGTEALAVDVVPAAVEDRGRTIGRIGVGLAENAQRRDEMFVTVRYGIVDALGKGLSQ
                     TWETTVMSLSVMRRMIVGEVSVKNLSGPVTIADFAGQSARMGLPHYIKFLALISISLG
                     VLNLLPIPLLDGGHLLYYVLEIIKGGPVSDRTMEIGQQIGMALLAMLMAFAFYNDINR
                     LVAS"
     gene            complement(26552..27748)
                     /gene="ispC"
                     /locus_tag="PKX01_06225"
     CDS             complement(26552..27748)
                     /gene="ispC"
                     /locus_tag="PKX01_06225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HMZ75757.1"
                     /translation="MKKLITVLGATGSIGVSTLDVVARHPDRYGVFALTAHRQVDKLY
                     EQCLAHRPAYAVVERAEDAVALQRRLDAAECRCDVLYGPAALCDVASAPDVASVMAAI
                     VGAAGLPPTLAAARAGKRVLLANKEALVMSGALFMAEVDTHGATLLPIDSEHNAIFQA
                     MPSGHARPLETGGVTQILLTASGGPFRSTPISDLPAVTPDMACAHPNWVMGRKISVDS
                     ATMMNKGLEVIEARWLFAAPAERIQVVIHPQSVIHSLVAYSDGSVLAQLGNPDMRTPI
                     AHALAYPERIDSGVKPLDLFEVARLDFERPDFARFPCLALAYRALQAGGVAPAVLNAA
                     NEVAVAAFLDGHLRFDRIAAVIADVLDAVPDAAADSLEVVFAADREAREVAASRVRAY
                     LAGARP"
     gene            complement(27761..28588)
                     /locus_tag="PKX01_06230"
     CDS             complement(27761..28588)
                     /locus_tag="PKX01_06230"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ75758.1"
                     /translation="MLKTRVITALCLLAGILAALFLLPEPAWVVFCALICAAAAHEWA
                     GMIGFAAARRAVYSVLLGALCLAGGCVAGLHRPETVAPFALAPVYGVSALFWLTCVPV
                     WLKSRWQLRGVGAGALVGLVLLLPPSLAIAHLRLLGPWVLLAVMAAVWVADIAAYFSG
                     RAFGSRKLAPSISPGKSWEGAYGACVGVVVYGFACAASVGLDWTAPRVWMPGVIGLMG
                     FTAVSIIGDLFESLIKRQAGIKDSGTLLPGHGGVLDRIDSLTSTLPMAALVALWLAR"
     gene            complement(28581..29369)
                     /gene="uppS"
                     /locus_tag="PKX01_06235"
     CDS             complement(28581..29369)
                     /gene="uppS"
                     /locus_tag="PKX01_06235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMZ75759.1"
                     /translation="MVRLPFLSSTRGIPEVVEVPRHIAIIMDGNGRWARKRLMPRVAG
                     HAKGVESLRAMIRTCQDLKVEYLTVFAFSSENWRRPKDEVTFLMKLFLRTLKEEIERM
                     HRSNIRLRVVGDISRFDSALVNLIEESETLTAGNTAMNLTIAANYGGRWDILQAVEKM
                     LAASPDAREGFTEDQLAEYLALSFGPEPDLFIRTGGEQRISNFMLWQLAYTELYFTDK
                     LWPEFDEAALKEAIRSYQGRERRFGRTSEQLDAGAGGPDSSDHA"
     gene            complement(29413..29970)
                     /gene="frr"
                     /locus_tag="PKX01_06240"
     CDS             complement(29413..29970)
                     /gene="frr"
                     /locus_tag="PKX01_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMZ75760.1"
                     /translation="MIPELKKTTEHKMLKSIEALKADLAKVRTGRAHTGILDHVMVEY
                     YGNPVPVNQVANVTLIDARTIGVQPWEKPMVGKVEKAIRDSDLGLNPAAQGDIIRVPM
                     PPLTEERRKELIKVVKHEGEGAKVAVRNLRRDANSSLKDAVKAKTISEDDERRAQDDI
                     QKLTDKAIVEIDKLLVQKEQELMQI"
     gene            complement(29981..30697)
                     /gene="pyrH"
                     /locus_tag="PKX01_06245"
     CDS             complement(29981..30697)
                     /gene="pyrH"
                     /locus_tag="PKX01_06245"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMZ75761.1"
                     /translation="MSQPAYKRILLKLSGEALMGDDAYGINQDVVTGIVTDVAEVVQL
                     GVEVGIVIGGGNIFRGMAGAASGMDRATADYMGMLATVMNAMALGDAFRVAGVNARVQ
                     SALRIDQVVEPYVRGKSIRYLEEGRVVIFGAGTGNPFFTTDTAAALRGAEIGANIVLK
                     ATKVDGIYTADPKKDPTATRFQRISFDEAISRNLAVMDATAFALCRDQRLPINVFSLF
                     KVGALKRVVMGEDEGTLVYC"
     gene            complement(30713..>31597)
                     /gene="tsf"
                     /locus_tag="PKX01_06250"
     CDS             complement(30713..>31597)
                     /gene="tsf"
                     /locus_tag="PKX01_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMZ75762.1"
                     /translation="TASMVKELREKTDAPMMECKKALAEAAGDMAKAEEILRVKLGNK
                     ASKAAARVTAEGIVGVYIGEGGKLAAIVEVNCETDFVAKNDDFLALTKKLAELVAAAN
                     PADVEALSALTIDGQSVEEVRKALVGKIGENMSIRRFVRTEAKGAVASYIHGGAKIGV
                     LVDLVGGDEALAKDIAMHVAAAKPKALDASGVPSELLDVERRIAIEKAREAGKPEAML
                     EKIAEGTVQKYLKDVTLLDQVFVKAEDGKQTIAQLLKAKGASVAGFTLFIVGEGIEKK
                     VTDFAAEVAAQAAAAAQK"
BASE COUNT         5372 a        11344 c         9784 g         4997 t
ORIGIN      
        1 cccagggcgg cgccgagctt gtcggccagg ggttcgagca gacgatggta gttttcgccg
       61 ctgcccaccc cgcgcccgcc ggaaacgatg atcttggccg cacccagctc aggccgggcc
      121 gacttggtga gctcacgact gaccagcttg ctctggccac tgtccgcgac ggcatccact
      181 gcagccaccg ccgcgccgcc accctcgccc gccgcctcga aggccgttgt gcgcacggtg
      241 atgaccttga cggcgtccgc actcttcacg gtcgccaggg cgttgcccgc atagatcgga
      301 cgtacgaagg tgtccgatgc ctcgatgccg acgatgtcgg aaatctgcgc cacgtccagc
      361 agtgccgcga cgcggggcag gacgttcttg ccgaaggtgg tggccggcgc gagcacgtgg
      421 ctgtaaccac cggcctggac caccgcgagg atctgggccg ccacgttctc tgccgactgt
      481 tcgcccaggt gggctgcatc ggcgacgagc accttggcca agccagccag cttcgcggca
      541 ctttcagcca caccgccgac gcccgcgccc gccaccagca ggtgcacctc gccgcccaga
      601 cgggccgccg cggtgacggt gttgagggtc gcgctcttca gcgcggcatt gtcatgttcc
      661 gcaacaacca gaatcgtcat cacagcacct tcgcttcgtt cttcagtttg tccaccagct
      721 gaccgacatc agccaccacc acgccggcgc tgcgcttggc cggctcggcc accttgaggg
      781 tggtcagacg cggcgtcacg tccacgccca gatcggcggg tttgacggtt tccagcggct
      841 tcttcttggc cttcatgatg ttgggcagcg tggcatagcg cggctcgttc aggcgcaggt
      901 cggtggtcac caccgccggc agcgcgatct cgagggtttc cagaccaccg tcgatctccc
      961 gcgtcacggt cgccttgccc gcgcccaccg ccaccttgga ggcgaaggtg gcctggggcc
     1021 agccgaggag cgcggcaagc atctgcccgg tctgattgga atcgtcgtcg atggcctgct
     1081 tgccgagcac caccaggctg ggctgctcct tgtccaccag ggccttgagc agcttggcca
     1141 cggccagggg ctgaagctcc acgtcggtgt cgacgaggat gccccggtcg gcaccgatcg
     1201 ccatggcggt gcgcagggtc tcctggcagg cgccgacacc gcaggagatc gccacgacct
     1261 cggtcgccac gccggcttcc ttgagacgca cggcttcttc gaccgcaatt tcgtcgaagg
     1321 ggttcatgga catcttgacg ttggccacat ccacgccgga cccgtcgctc ttgacccgga
     1381 ccttgacgtt gtaatcgacc actcgcttaa cgggaaccag aatcttcaat gctgtgctcc
     1441 ttcctcaaaa aggttctcca ccaacgctta tgcgaccgcc gcaggcaaaa ctccgcctgc
     1501 ctgcacggcc gttcttctgg atacgccgac gctcgggacg gcgcctgaac cgcaaaccgg
     1561 cagcttctca cacgcccgct tgctttgtca ctttccatac ggtagcgtat ggacgacaaa
     1621 cctgtcaata ctacgccgta tggaaaaaac gacccgcccc cagctcgacc gcgccgcctg
     1681 gatccaggcc gccctggaag tcctcgccga cgaaggcgtg cagggcatgc gggtggaagt
     1741 cctcgccaag cgtctgcgcg tgaccaaggg cagcttctac tggcatttca aggatcgcca
     1801 ggatctgctg tccggcgtgc tggagacctg gaaagccggc cgcatccagg acatcgtcaa
     1861 gcagacgcgc ctcgaaccgg gccgggagcg ggcccagatc gcccgcctgg tggacatcta
     1921 cagcaccagc cgcagccgca agggcctgct catcgaactg gctgtgcggg aatgggcgcg
     1981 ccgcgacgcc gaggccgccg ccatcgttga agaggtggac gcctggcgcc tgcgctgcgc
     2041 ccgcgagctg ttcctggcct gtggcctgcc ccagcacgag gcctcgaccc gcagcatgct
     2101 gctctatgcc tacgttttcg gcgcgtccat catgatctac gaccgcttcg acgcggacat
     2161 cccgcgcctc acggaagaca tccgcgcgct gatcgtcggc ccgccggcgc cgacgttggc
     2221 gtgatgcacg gcatcaacgc aggccggcaa tcacggccac ggcgttgcgc cccgtcgcgg
     2281 cgatcagctc cgcctcgtcc atgccccgca gcgccgccag ctcggcggca atacggcgca
     2341 gattggccgg caggttacgc tgccgggcgg cccacgccgg cggaatgtcc ggcgcatcgg
     2401 tttccagcac cagcgcgtcg aggggcagcg ttgcggccag ttcgcggatg cgccgcgaac
     2461 ccgaataggt catcgcaccg ccaaacccca gcttgaagcc cagcccgatg aaggcctcgg
     2521 cctgctggcg gcttccatta aaggcatgag cgatgccgcc ccgcaccctg atccgccgca
     2581 gggcgcccaa cactgcgtcc tgggcccggc ggatgtgcag gaccaccggc aggtcgaacg
     2641 tccgcgccag gcgaagctgc gcctcgaaga attccgtctg ccgcggcaag tcaccgtccg
     2701 gcacgtaagc atcgaggccg atctccccca ccgccaccgc ggagccgtcg gcgagccgcg
     2761 accgtatacg ttcgagatcg tccgcctgcg cccgcgccac gtacatcgga tggatgccga
     2821 aggcgtggcg gcagtccgcg tgggccgccg acagcgcggc cacggcgtcc aggttgcccg
     2881 cgtccaccgc cggcaccagg aagcggccga cacccgccgc ccgcgccgcg tcgatctgcg
     2941 ccgaccgatc gtcggcgaat tccgcggcgt ccagatggca gtgggtgtcg atcagcacgc
     3001 gggccgcctc agcggccggt ccacaccggc gtgcgcttgg ccatgaaggc gtcgataccc
     3061 tcggccgcgt cctcgcacat catgttgcag gccatggcct cggcggccat ctggtaggcg
     3121 gcgtcaagcc ccatctccag ctgcgcatag aacatgcgct tgcccatcgc gatggcggcg
     3181 ggcgacttgg cgcagatgga ggcggtcagc ttcgcgacct cgtcgtcgag ttgctccaaa
     3241 ggcaccacgc gattcaccag accacggcgg cgggcttcct gggcgtcgat gaaatcgccg
     3301 gtgaccagca tctcgaaggc ttccttgcgc cccatgttgc gggccaggcc cacgctgggc
     3361 gtggcgcaga agagccccac gttgatcccg gacacggcaa agcgcgccac gtccgaggcc
     3421 accgccagat cgcacatgga caccagctgg cagcccgccg ccgtagcgat gccgtggatg
     3481 cgcgcgacca ccggctgcgg catggcgacg atcttcatca tcacctttcc gcacaggcgg
     3541 aacagggcct gctggaagga cttggtgtaa ttgccgcgca tttccttcag gtcgtgaccg
     3601 gcgcagaagg ccttgccggc gccggccagc accacgacgc gcaccgcgct gtcctcggcg
     3661 atgcgctcca gctccgccag caaggcttcg agcatggcct gggacagggc attgaactgg
     3721 cccggacggt tcagggtcag ggtggtcacg ccgtccgcgt cggtgcgcag cagcatgggt
     3781 tcctgggggg tgtcgacgac tgcgctcatg agttctcctc cgtactttga aaatgccggc
     3841 tcgaaatggc cggtgaaaaa aatcggttcg cccggattta ctggctgcat cgcgctccga
     3901 cgctgcccgc cagaccgtcg agccaagtca acagggccgc gtcgaacgcc tggacggcgt
     3961 cccgcagcgc cacgacgcct ccggccgcat ccgggctcga cgccggacgg gtcacgctga
     4021 agccctgggt ggccaggagc tggtcggtcc gcggcgcgag cagggacgcc cgcgcgtcga
     4081 tgacaccccg gctgcctgac tcattgtcga agacctgcat gaactcgtcc agatcgaccc
     4141 gcagacggca gccgccgaga ctcacggtgg tcacgttaac ccgcaccgaa cgcttcaggg
     4201 ccacctccag caactgaccc ggctgcgccg cccagcggct ctgcacgaag gcctgacggc
     4261 gcgtctcctg ggcgtaggcc agtcggtact gcatggccgt ggacgccagc cagggcgcgg
     4321 ggatcacctc cagcgtgcgg atcgggtagg gcagactgcg gggcccgacc ggcgccagcg
     4381 ggccaaaatc atgcacggcg aaggtcgggg cgccctttgg cagcacggcg ccacagcccg
     4441 ccaggcttac aagggccacg acgaacgaca cagacgccga aaggcaggca cgcagggaat
     4501 gcgtcatgga tggctcatgg tcgattcggg acaaatcccg cctccccggg ccccggcggg
     4561 gccttgccgc gcccgagcag gaagatctgc ggatgggaat ccagctcgtc gagcagatca
     4621 ttcatctgcc gcgaggtggt ggtcagctcc tgcagcagca cgttgaggcg cggcagggtg
     4681 ctggtggcga agccttcgcc ggtggaggcg gcgatcacgt caagacggtc ggccacatcc
     4741 tgcatgcgcg ccagcagacg gcgaccgtcg gcggccagcg gcgcggcatc gctgctgaag
     4801 cgttcggcat tggccaacgt acgttcgatg cgcgccaggt tgtccttgcc gagcatccga
     4861 tgcaccgccg ccagggacgc cgcggcatcc tgcatgccct tgtccagacc tttggaagac
     4921 gactccatat gcgccaggat gcgctccacg cggccgatgt tctcgttgct ggcgagccgc
     4981 gcaaagcgtt cggacagctc gcgcagctgg cccaaggtgc gccgcgtcgc atcggccacc
     5041 tcttccacca ggcctggctc cagggcaatg cggggcggcc cgttgccgtc ggccgcgagg
     5101 ggtgtcggat cggcgccgct gtcgtcgagg gcaatgaagg cgatgccggt gacgccctgg
     5161 gtcgccagac gcgcccgcgt gccgtgggtc aggggcagat cggcacggat gcgcgcggtg
     5221 accagcagat cctgcggccg cgccgggtcg agggcgatgc tcgccacctt gcctgccatg
     5281 atgccccggt agcgtaccgt ggcctgggga ttgaggccat tcacgctgcc ggcgctgacc
     5341 agcaccacgt cgcgggtcac ctcccgcttg tccgagaacc accacagggt gaacaccagc
     5401 gccgtaccca gcagcagcgc gaacaggccg gcggcgatgg cgtgggcgcg atcttccatc
     5461 aggcctccgc ccgcccccgc gccagcgccg cacgactgcg ctcgcctcgg aagaaacgtt
     5521 cgatgaaggg atgctccagt gccatgacgg aatccaggga accgaagccc agtatacgtc
     5581 cttccgccag cacggcgacg cgggtcgcca gggcggccag ggtatccaga tcgtgggtca
     5641 ccagcaccac cgtcaggccc agcgcccggt gcaggtcgcg gatcagacgc acgaagcccg
     5701 cgctacggtc cgggtcgagc ccggcggtgg gttcgtcgag gagcagcagc tcaggctcca
     5761 gggccagcgc ccgcgccagg gccacccgct tgaccatgcc gccggacagc tccgcgggca
     5821 tgcgcagacc cacctccggc gccagctcca ccatggccag cttgacgccc accaggtcgt
     5881 ggagcatcgc ctcgtcccga acacctgctt cctgcaacgg aaaggcaatg ttggcgtaga
     5941 cgtcgagggc cgagaacagc gcgccctgct ggaacagcat gccgaagcgc cgccgccggg
     6001 ccacctgctc gaggcggctg ccaccgaaca ggggttcgcc gaacaggcgc acctcccccg
     6061 cggtaggacg cagcaggccg acgatctgac gcagcagggt ggtcttgcca ctgcccgagc
     6121 cacccaccag ggcgacgatc tcacccgccg gcaccgccag gtcgatgccg cgatgcaccc
     6181 aggtgtcgcc aaaacgggtg gacagcccct tcacctctac cgggaaggcg ctcatcctgg
     6241 caccccgatg gagcgggtgg cgatggcaaa tacggcgtcc gtcaggatca ccagcgtgat
     6301 cgcggtcacc accgatgccg tggtatgggc cgccagactc tccgtattgg gcttgacccg
     6361 caagccgaaa tgacaggcca ccagagcgat cagggcaccg aacgccgcgc ccttggacaa
     6421 cccgatcacc agattgggaa agggcacggc ccgcggcagc gcctgcagga agtactcgaa
     6481 gccgatcccc agctggagcc aggccgacac catgccgccc acgatcgcca ccgccgagca
     6541 ccacagggtg agcagcggca tcgccaggaa cagggccacc accttgggta gcaccagtcg
     6601 cacgtaacgc gagatcccca tggtcgccag cgcgtcgatc tcttcggtga cccgcatcac
     6661 gccgagctgg gcggtcatcg ccgaacccga gcgcccggcc accagcaccg ccaccagcac
     6721 cgggcccagt tcgcggatga tgcccaggcc aaggatgttg acgatgtaga tgtccgcacc
     6781 gaaggcccgc agctgtagcg ccgacagata cgacagcacg acgccgatca ggaagcccac
     6841 cagcgccgtc accggcatgg ccttgacgcc caccttgtag aaattggccg acatctcgcg
     6901 ccacggccag cgctggcgat cccccagcag atggagggcg tccagcagga tctgcccgac
     6961 cagggcggtg aaatccgcca gatgccgccc gaagccaatc gccagacggc ccagatgcgc
     7021 cgtcgcgtcg agaaagcggt aaggcggcgc gaccggcacc gacggccccc ccagcgccgc
     7081 aagacggaca aagttttcgt cgaggatggt cggcacctgc agacgggccg gtcgttgccg
     7141 gccccagtgg cgccacagca gggtcgcgcc aaaggtgtcg agacggatga ccccgtccag
     7201 atcccagacg gccaccccgt gcacctcggc gagggccgcc tgcaccaccg acaggcgcgg
     7261 cagcaatgcc cgcagggtcc agtcgcccgt cagggtcacc gtcgccgcgg cgccgtgacc
     7321 gtccacccgg agcgccggcg ctgcctgcgg ggccgcattc atgggctacc gcacgccatg
     7381 tgacgcctca ggccgccccg cgcgccgccc gttcgacgcg cacccgcagc ggcatgccga
     7441 tgccctgcca ctggcgctct tcctcgtcca gggccgccgc cgtgagcggc aggttgcgca
     7501 gccagtcacc gtccgccagg agcacgtagg cgcggccttc gcgcttcacc cgcaagggcg
     7561 gcacgccgcg gccgtcgcgg gcgcgatgca ccaccgccgc cagacgcagg cacaggatga
     7621 agcgccactc caggctttcc ggatccagtg cgctgacccg cgcgagcttg ccccggtggg
     7681 ccagcaccat gcgcgacagg cgtccctggt ccatccgcga gaagcccggc atgtcggcgt
     7741 tgccgaggat gtaggcactg tgtttgtggt agctggaatg ggccaccgac acgcccacct
     7801 cgtggagcag cgcagcccat tccacgaagc ggcgatccgt gttctcggga tcttccgtcg
     7861 atggatcgag ctgctccagg aaatgcagcg cggtatcggc gacccggcgg gcgtgggcga
     7921 catccacgct gtaacgctcc atgaaggccg tgacggtggc gtcgcgcaga tcgtggtggt
     7981 ggtagcggcc gagcaggtcg tagagcactc cgagacgcag ggcaccttcc gaaaacacca
     8041 tgcgctccag gccgaactcc tggaagatcg ccgacatgat ggcaaagccg ccgggcagca
     8101 ccggcagtcg atccggccgc aggccttcca gctgcagggc ctggacgctg ccggccttga
     8161 gcatcagcac cttgagcttc tccagcccgt cgcgggtgat gccgccagcc gacaggccgt
     8221 tgagctcgag gatctcgacc agcgccttgg ccgatccgct cgacccgacc gcctcttccc
     8281 agccggtgcg gcggtaggcg tggctgatgg tctggatttc cttgagcgcg gccagctcgg
     8341 cttcccgcaa ccctttcttg tccacccgcc cgtccgggaa gtaacgcagc gaataaccca
     8401 cgcagcccat gtacagggac tccagttcca gcggcttgaa gctcttgccg atgatgaact
     8461 cggtggagcc gccgccgata tccaccacca gttgctggcg gtgcggattg ggcagggtat
     8521 gggcgacgcc cacgtagatc aggcgggctt cctcgcgacc ggcgatcact tcgatgggaa
     8581 atcccagcgc cgcttcggcc tggatgagga agtgtggcgc gttcttggcc acccgcaggg
     8641 tgttggtggc caccgcgcgc accgcctccg gcggataatc ccggatccgc tcgttgaagc
     8701 agcgcaatgc gttgaggccg cggaactggg cggcggcgtc cagcatgcgg tcgggcgaca
     8761 gaccggccgc gaggcggacc gactccttga tgccgtcgag aggatagatt tggtcgttta
     8821 caatccgtcc gacctggagc cggaagctgt tggaacccag gtcgatggcg gcgatcagtt
     8881 cgtggttcat gaatcggtcg caaggtgcgc cggaaggcac gcaggggctg cattctagca
     8941 ccccaccgtc cgcaccgccc ttcttcgccg ccgtcacacg atcgcaataa aactgtcaca
     9001 gaatcctcgt ccggagagac aacagaacaa ggatccgccc atgcgccccg cccccgcacc
     9061 ccgctacagc accgatcact tcatcaatcg cgagctgtcc ctgctccagt tccagcgccg
     9121 ggtgctcgcc caggcggcgg atcccgccgt gccgctgctg gagcggctgc gcttcctgtg
     9181 catcgtttcc agcaacctgg acgaattctt cgagatccgc gtggccggcg tgaaggagca
     9241 gatgcgcctc gggcgggtcg ccaccacggc cgacggcacc tcgccgcggg tgctctacga
     9301 gcacatcagc cgggaggtgc acagcatcat cagcgagcag tacgcgctgc tcaacgacac
     9361 catcctgccg gcccttgccg ccgaaggcat cgtattcctg cgccgggtgg cgtggaccga
     9421 ggcccagcac acctgggcgc gggattactt cctgcgcgag gtgatgcccg tcctcacccc
     9481 catcggcctc gatccggccc atcctttccc gcgggtgctc aacaagagcc tcaatttcgc
     9541 cgtggaactg gaaggccgtg acgccttcgg ccgcaactcc ggcgccgcca tcgtccaggc
     9601 gccccgcgcc ctgccgcggg tcatccgcgt gccgcgggag ttggccgacg ccgaatacac
     9661 ctacgtcttc ctgtcgtccc tgctccacgc ccacgtgggc accctgttcg aaggcatgaa
     9721 cgtgcagggc gtgtatcagt tccgcgtcac ccgcaattca gacctctacg tggacgagga
     9781 agaggtgaag gacctgcgcg ccaccctcaa gggcgaactc cagcagcgcc acttcggcga
     9841 cggcgtgcgc ctggaagtgg ccgacaactg ctccagcgcc atggccgact tcctcaagag
     9901 ccagttcggc cttggcgacg cggacgtgta tcgcgtgccg ggcatcgtca atccggtgcg
     9961 cctgatccag gtgccggact gggtggaccg cccggacctg aaataccgcc ccttcctgcc
    10021 ggcgcgtccc aagggcctcg acaagcgcga caacatcttc gctgccatcc gccgccagga
    10081 catcctgctg catcatccct tccagagctt cgagccggtc atcgagctcc tccaggccgc
    10141 ggccgacgat ccgcaggtga tggccgtcaa gatgaccgtg taccgcaccg gcaccgactc
    10201 cgtgctgatg gaacacctcg tccgcgccgc ccagaagggc aaggaagtca ccgtggtgct
    10261 cgaactgatg gcccgcttcg acgaagaggc caacatcagc tgggccgccc gcctggaaga
    10321 ggccggcgcc cacgtggtct atggcgtgtt cggctacaag acccacgcca agatgctcct
    10381 gctggtgcgt cgtgaggacc gcgggctgaa gcgctacgtg cacctgggca ccggcaacta
    10441 ccacccacgc acgacccgct tctacaccga cttcggcctc ctcaccgcca acgaggagat
    10501 cggcgccgac gtggccgagg tcttcaagca gcttaccggc ctgggccagg cgggcaagct
    10561 gcgccacctg tggcaggcgc cttttacgct gcagcccaac gtggtcgccg ccatccgcaa
    10621 cgaagccgaa atcgcccgcg ccggccgcaa ggcccgcatc gtggtcaagg tcaatgccct
    10681 gctcgaagcg gaaaccatcg aggccctcta cgacgcctcc tgcgccggcg tggacatcac
    10741 agtgatcgcc cgcagcgtgt gtgccctcaa gcccggcgtg cccggcctct ccgagaacat
    10801 ccgggtccgc tcggtggtgg gccgcttcct cgaacaccac cgtatcttct acttccacgc
    10861 cgacggcgcc gagaagctct acctctccag tgccgactgg atggagcgca acttcttcaa
    10921 gcgcatcgag gtggctttcc ccatcctcga ccccagggtc aagcgacgcg tcgtcaagga
    10981 gggcctgcgc ccctacctgg gcgacaactg ccaggcctgg gagctggacc aggacaacca
    11041 ctaccggcgc aagaatcccc gcaccagccg gcgcgccgcc caggaactgc tcctcaagga
    11101 cgtggccggc gtgaccctcc cgtgaggacg cggccgcgcc ggacatatcc gcttacggcc
    11161 ggacaggcgg gatcggcccg gccgtgatgt agaatgtggg acttcccacg aacagtcctg
    11221 gccaacccca tgtccctgcg tcccgccttc ctgtcccggc gccgcctcgt cgtcggcgcc
    11281 ctgctcggtt ccgtcatcct cgccggctgc ggtggcaagg gcagttcagc caatgcaccg
    11341 acgggcggca tcaccgtgac ccccggcgac acccaggtca ccatcacctg gcaacaggac
    11401 gccggagtcg aatactggct ctggtatgcg ccgggcagca ccatcaatcc gagcgactcc
    11461 aacacgccgg gcatccgcta tcacatcaac atcggctcgc cctacgtcgt caccggcctg
    11521 accaacggca cccagtatgc cttcgcgctc aacggccgga tcaacggtgg caagggcggt
    11581 gactacgcat acgggacgac caccccacgt ctcgccggca ccacctggac ggccgtcacc
    11641 aaccttaccg ccgacatgcg cggtctcgcc tacggcacca cctccacggt caccaccgcc
    11701 acctacgtgg cggcaggcac agctggtgcc gccttctcgt ccaccgatgg tcacacctgg
    11761 acggccctcg ccaatcccgc cggcaccgcc aacgtcaatg ccatgaccgt cggcctgtct
    11821 accttcatcg cggtcggcga cggtggcgcc atctactaca gcaccgacct gtccacgtgg
    11881 acccaggcca cctccaacac caccaacaag ctcaccgccg tcgccacctc cggcggtgtc
    11941 gcagtggcgg tcggcgagaa cggcacgatc cgctattcca ccagcgccgg cacctggacc
    12001 gctgcggcca gcgtaccgtc cggcatcggc cacctgtacg gcatcagcta caccccgtcc
    12061 ggcctgtgga tcgccgtagg cgccggcggt gtggtgctga ccagctcgga tggcaccaac
    12121 tggacccaac gcacctccgg caccgccgcc accctctacg gagtgtccgg catggccacc
    12181 acctccggca gcaccaccac ctatacctac gtggccacgg gcgcgggcgg tcaggtcctc
    12241 accagcagcg acggcaccac ctggaccgcg tccaccgtct cgccggcggt caccctgtat
    12301 tccgtcatcg gcagcaacca gttcgtcgcc gtgggcgcca acggcgcggc cttcaccagc
    12361 cccgacggcg tgacctggac ctactacgct gccagcagcg gcaacgacct ctacaccctc
    12421 atgaaggccg ccaaccagta catcgcggtg ggcgccaaca aggccatggc ctactcccag
    12481 taagccccgc cggcccggtc tccgagtgga gcgcccgcaa ggccgctcca ctcggtcctt
    12541 tcggacccgg aaatcgaacc tgcagcgccc cgccccatgt ccgccaacgc cgccctcctc
    12601 gcccgctccc tcgccgccgt ctggcacccc tgcacccaga tgaagcacca cgagcagctc
    12661 ccgctgatcc ccatcgcgcg gggtgagggg ccctggctct acgacatgga cggccggcgc
    12721 tacctggacg gcatcagctc ctggtgggtc aacctcttcg gccatgcgaa cccgcgcatc
    12781 ggccatgcca tcaaggacca gctcgaccgc ctcgaacacg tcctgctggc gggcttcacc
    12841 cacgaaccgg tcgtcgagct ctccgagcgc cttgccgccc tcaccggcca ccgcctcggc
    12901 cacgccttct acgcctccga cggcgcctcc gccaccgaga tcgccctcaa gatgagcgcc
    12961 cacttctggc gcaatcaggg gcagccccac aagaaccgct tcgtctgcct cgccggcagc
    13021 taccacggcg aaaccgtcgg agccctcgcg gtcaccgacg tggccctgtt ccgcgatgcc
    13081 tacgccccct tgatccgccc ggcaagctgc gtacccaacc cggacgcacg ccaggcgatg
    13141 cccggcgaaa ctgccgtaga tgtggcccgc cgcgccgccg ccgcactgga aaccttcctc
    13201 gccgaacatg ccggcgagac cgccgcgtta atcgtcgaac ccctcgtcca gtgcgcctcg
    13261 ggcatggcca tgtacgaccc ggaatacctg cgtctggccc gcgccctgtg cgaccgccac
    13321 caggtacacc tcatcgccga cgagatcgcc gtcggctgcg gccgcagcgg cagcttcttc
    13381 gcctgcgagc aggccggcat cacaccggat ttcctgtgcc tgtccaaagg catctccggc
    13441 ggctacctgc ccctgtccat cgtcctcacc cgcgacgccg tctacaacgt cttctacgac
    13501 aacgacaccg cccgcggctt cctgcactcg cactcctaca ccggcaatcc cctcgcctgc
    13561 cgggccgccc tggcgaccct ggacatcttc gccgaggacg aggtgatcgc cgccaaccgc
    13621 caccgcgcca cccgcctcgg cgaggccttc cgcgccgctg tcggcgacca tccgcgggtc
    13681 cggcatctgc gccagcaggg catgatcctc gcattcgacg tggccgacgc cccgcccgac
    13741 ttcgcccgcc gctactttgc cgaagccctc cagcgcgaac tcctgctgcg ccccatcggt
    13801 cccaccgtct acaccctgcc gccctacatc ctcgacgacg tgcacatcga cttcctcgcc
    13861 cgccgctcgg ccgaagccct ggaggccgca cttgccggct gaggcccacg accccctcga
    13921 cgcccttgcc accggccttg cagagctgga cgccgccggc ctgcgccgcc gccgccgcgt
    13981 cgtcgccagc ccctgcgcgc cggccgccac cctggccgac ggcagcgcgc gcattgcctt
    14041 ctgctccaac gactacctgg gcctcgcgtc cgacccggcc ctcatcgacg ccgcccgcgc
    14101 cggcgccgcc cgctggggcg tgggctccgg agccgcccat ctggtgagcg gccacacggt
    14161 cgcccacgat gccctcgaag cccgcctcgc cgccttcacc ggcttcgacg ccgccctctg
    14221 cttcaccacc ggctacatgg ccaacatcgg cgtcatgccc gccctcgtcg gccgcggcga
    14281 tgccgtcttc gccgaccgcc tcaaccacgc ctccctggtg gacggcgccc tcctcagccg
    14341 cgccgatctg caccgctacc cccacggcga cgtggacgcc ctcgcccgcc agctcaccgc
    14401 ctccagcgcg ccgcgcaagc tcatcgtcag cgacggcgta ttcagcatgg acggcgacat
    14461 cgccccgctg ccccggctcc tcgaactggc cgaaacccac aaagcctggc tgctggtgga
    14521 cgacgcccac ggctttggcg tgctcgggcc ccagggccgc ggcagcctgg cccacttcgg
    14581 cctgcgttcg ccgcgcctga tctacatggg caccctgggc aaggctgccg gcgtggcggg
    14641 cgccttcgtc gccgcccgcg ccgacgtcat cgactggctg ctcaacaagg cccgcagcta
    14701 catcttcacc accggcgcgc cgcccctgct cgccgacacc ctgctcgccg ccgtcgatct
    14761 catcgaacag ggcgacgccc ggcgcgccca cctgagcgcc ctcatccagc gcctgcgcaa
    14821 cggcctcacc ctgcgccgct ggcatctgat gccctccacg accccgatcc aacccctcgt
    14881 catcggcgac aaccacgaag ccctcgccgt cgcccgcgcc ctcgacgagg ccggcctgtg
    14941 ggtgcccgcc atccgcccgc ccaccgtgcc caaaggctcg gcacgcctgc gcatcaccct
    15001 gtcggcggcc cacacgatcg aacaggtgga tcgcctcgtg agcaccctca ggcgcctgga
    15061 gggcggcgcc ccgtgagcgc cctcgtcctc ctccacggct ggggcctgag ccaccgggta
    15121 tgggcgcccc tcctcgcggc cctgccgggg cacgcgatgc tcacccccga cctgcccggc
    15181 cacggcgacg ccccgcgcgc cggcccgcgg ctcgcggatt ggtccgacgc cgtcgccgcc
    15241 ggcctgccaa aaggctgcac gctggccggc tggtccctcg gcgcgctgct cgccctggac
    15301 atcgcccgcc gacatcccga caaggttgcg cgcctgatcc tcaccggcgc gagcccccgc
    15361 ttcaccgcca ccgacgactg gccctgcgcc ctggcggccg acaccctgcg cgacttccgc
    15421 actgccttcg ccgccgagcc ggacgccacc cagcgccgct tcgtcgccct ccaggccctc
    15481 ggcgaccacg cccggcgcaa cgtcacccag acgctcaccg ccgcactcac cccggccgac
    15541 accgcccatc gcgaggccct cgccgacggc ctcgccctgc tggccgacac cgatctgcgc
    15601 cccatcctgc cggccatcgc ccagccggta cgcctcctcc atggcgcccg cgacgccctg
    15661 atgccggtcg ccgccgccga acacctggcc gacgccctgc cccatgcccg cctgagcatt
    15721 ttcgccgaca gcggccacgc gcccttcctg tcacgcccgg acgatttcgc caccctcgtg
    15781 gaaggcgccc gccatgactg acgcgcccgc ctccaaacgc gccgtccgcg ccgccttcga
    15841 ccgggccgcg gccgcctacg attcggtggc ccacgtacag cgcagcgcct gcgaccgcct
    15901 cttcgccctc ggcacgcccc acctttcaac ggccgctcgc gtcgtcgatg ccggctgcgg
    15961 caccggctat gccctgcccg gcctgcggca gcgcttcccc gacgccgaac tgtgcgccgt
    16021 ggatttcgcc cccgccatgc tcgcgcgcct gcccgccggt ctcgctcacc ccctctgcgc
    16081 ggacctggaa cagctgcccg tcgccgatgc ctgcgtggat ctgctgtggt ccagctacgc
    16141 cctgcaatgg tgtgcgccgc gccgggccct gaccgacatc gcacgggtgc tgcgcccggg
    16201 cggccaggtg tggctggcca ccctggggcc cggcaccctc ttcgagctgc gcgatgcctt
    16261 cgccgccgta gacgacgccg agcacgtgct gcgcttccag ccgccggagg tcgtcgagga
    16321 cgccgccgag tccgccggcc tgcgcctgct cgccagcgag cgggacaccc tgcaggcctg
    16381 ggcgccggac ctgcgccgcc tgctccacga catcaagacc ctcggcgccc accagaccgg
    16441 cgccacgcga cgtcccctgc gcaagaccga cctggcccgc ctcaccgctg cctacgaggc
    16501 ctaccgcacc ccggacggcc tgcccgcgtc ctatgacacc ctctggctcg tcgccgaaaa
    16561 accatgagcg cttacttcat caccggcacc gacaccgaga tcggcaagac cttctccacc
    16621 tgcgccctgc tccacgccgc ccgtgcccag gggctccgtg ccctcggcat gaagccggtc
    16681 gccgccggtg cggaatgggt gaacggcgaa ttcctcaacg aggacgcggc ccgtctccgc
    16741 gccgctggca gcttcgatcc cggcctggcg ttgctcaatc cctactgcct tgccagcccc
    16801 attgcgcccc acattgctgc ccgcgaggaa ggcgtgcgca tcgaacccgc ccgcatccgc
    16861 accgccttcg acagcctgcg cgcgcaggcc gacctgctcc tggtggaagg cgtcggcggc
    16921 ttccgcgtac ccctcggtga cgactacgac agcgcggacc tggcccgcga tctgggcctg
    16981 ccggtgattt tagtagtggg cctgcgcctg ggctgcatca atcacgccct gctcagtgcc
    17041 gaggccatcg ccgcccgcgg cctgaccttc gccggctgga tcgccaaccg cgtcgacccg
    17101 gccatgctgc gtgtcgacga gaatctggcg gccttgcaaa aggggctgcc ggcaccgctg
    17161 ctgggcgtcc tgcccttccg cgaagaccgg gacgccgccc tggctgccac ggccctgcgt
    17221 ctgccctgag cgcgccgggc ggcgttcagc cgccggaagg atgcaggccg cgctggcgca
    17281 cctgctcgaa caggcatacg ccggccgcca cgcccacgtt gagggattcc atgccaccgg
    17341 gcatggggat gcgcacccgg ccggtggcca gcgccgcaat cccctcgctc aggccctgac
    17401 cttccgaacc gaacagccag gccaccggcc cgcgcagatc ggtggcgaac agctccgtcg
    17461 cgtcctgcag ggccgtggcg aggattgcgc cggggtagcc cgccagcgca gccgacgcat
    17521 cgatgccctc gtcgatgtgc agcaggaaat gcgcgcccat cgccgcccgc agcacgcggg
    17581 gcgaccaggc ctgggcgcag ccgggggtga gcagcacctg ggcgatgcca gccgccgctg
    17641 cggtgcgcag gatggtgccc aggttgccgg ggtcctgcac gccatcgagc agtacgcagt
    17701 cacccgtcgg gctgttgccg gcctgtcccg ccgggagcgc catcacggcc agcaccccgc
    17761 tgggagaatc caccggactc acgtgggcga acagggcatc cggcaggaca agttgcgcca
    17821 catcgggcgc tgccgccagg agcgtaacga tctcggcctg cgcaaagccc gcgtcgctga
    17881 ccacgatctg gcgcagcgcc tgcccgcggg acagggccac ctgcaccaga tgggccccgt
    17941 cgagcagggt ctcgccggcc ttgcgccgct cccgcgccga ggtggcgagg gcgtgnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgag cagggccatc acgtgccagg
    18121 tgcgcggtgc ctgggcgaag cccacatcgg ccagcatggc aaagccggtc agcaggatgg
    18181 tggcgacggc cgctgccacc agcgggaaga aacgcccgaa gaccgcccgc cacaggctca
    18241 ggcgctgagc cggaggcagc tccagggccg ccggacgcag gcacaggtgg gcgaagacca
    18301 tgccacccat ccagacgatc acgccggcga ggtgcaggaa caggagcagg tgatggactt
    18361 tcatgggtgc ggatcctcgt tccacaggga agcttgggcg acgacggcac gcaccggccc
    18421 gaagctgcgc cgatagatgt cgcacgcgcc gtgagccttc agggcggcca gatgcgccgc
    18481 cgtcgggtag cccttgtggc cagcgagacc gtactgggga tagcgccggt caagggccac
    18541 cagcagtgcg tcgcgggccg tcttggcaag gatggacgcg gcggagattt ccgccaccag
    18601 gctgtcgccc ctgaccaccg cctcgcaggg caggtcgagg gccgggcagc ggttgccgtc
    18661 cacctggacg cgggtcggcc gcaggttcag gcccgccacg gcccgctgca tggccagcat
    18721 cgaagcgtgc aggatgttga ggcggtcaat ctcttcgacg ctggcttcag cgatatgcca
    18781 ggccagggcc tgggccttga tcgtccgggc cagggcctcg cggcgcgccg cagagagctt
    18841 cttggaatcc gccaggccgg cgatgggacg cgccgggtcg aggatcaccg ccgccgccac
    18901 caccgggcca cacaagggcc cgcggccggc ctcgtctacg ccgcacaggc gctccatcag
    18961 acgcgacccc gcagataagg caggatcgcc tcggcagccc gcgacgcggt gtcctggcgc
    19021 agttgccgat gcatgtcggt gaaggtctcg agcagctcgc cgcgccggcc tgcatcctgc
    19081 agccaggcat cgagggcgtc cgccagggct tggggcgtgg cggcatcctg gagcagttcc
    19141 ggcaccacct cgcgaccggc cagcacattg ggcagaccaa cccagggtag atacgccagg
    19201 cgcttcatca ggcgccagga ccacttgccg atccggtagg tgatgaccat cgggcgcttg
    19261 agcagggccg cctccagggt ggccgtgccg ctggccacca gcaccacgtc ggcggcagtc
    19321 atggcctcaa cggcatgacc gaacagggtg cgcaaaggca gtgtgaggtg ggcatggcgg
    19381 gccatcgccg cgtcccacag cagccgcgtc tcgcgggtcg ccaggggcac caggaaggtg
    19441 gcgtctggat ggcgctcgtg caggatcgcc gcggtggcga tgtaggtatc ggcgagattg
    19501 cgcagctcgg actgacggct gcccggcagc agggcgaaca ccggccccgt gccactcagt
    19561 tgcagaagtt cccgggcgcc ggcccgatcc ggcgccaaag gcagcacgtc ggcaaaggga
    19621 tggcccacgt agctcacggg cactccggcc gtgcggtaga gctcgggctc gaaaggaaac
    19681 agacacagca tgtggctcac cgcgcgggcg atgcccttca gccggccacg ccgccatgcc
    19741 caaatggacg ggctcacgaa gtggatcgcc gggatgccct tatccttgag gcggcgctcc
    19801 agccacaggc tgaaatccgg tgcatccacc ccgatgaagg ctgccgggcg gtcggccagg
    19861 atgcgcgcct tgagctggcg acggataccg gagagctcgc ggtagtggcg cagggcatcc
    19921 acatagccat gcaccgcaag cttttcggcc ggccacaggg cctggaaacc cagggcctgc
    19981 atcttgggcc cgccgatgcc atagaactcg gcatcaggca gatgacgacg gagcgcctgg
    20041 atgaggtggc tggccagcag atcgctcgac gcctcgcccg ccaccatggc gatgcggacg
    20101 gtcatggatt aacggacgat cccgcgaccg gaatcggcga tgaagtcgga aaacatttgc
    20161 agctctggca cattggcggc tgcctcggcg atttgccgac gcgcctcatc cagggtgaga
    20221 ccgctccgat agagcgtctt gtaggcgcgc ttgatggccg tgatgccgtc actactgtag
    20281 ccgcgccggc gcaggccttc gctgttgatg ccgtggggtg ccgccgggtt gccggacacc
    20341 gtgacgaagg gcggcagatc ctgcaacagc accgtcccga caccacagaa gctgtgggcg
    20401 ccaacgcgca caaactgatg cacgcccgtg aaaccgccaa gaatggccca gtcacccacc
    20461 gtcacgtggc ccgccagcgt ggcattgttg gcgaagatgg tgtgatcgcc gacgacgcag
    20521 tcgtgggcca cgtgcacata ggccatgatc cagttgtcgt gaccaacgcg ggtcaggccg
    20581 ccgtcctgga cggtaccggt actgaagctg cagaactcgc gaatggtgtt gccgtcgccg
    20641 atttcaagcc gcgtcggttc gccggcgtac ttcttgtcct ggggttcgga gcccagcgag
    20701 cagaactgga agatgcggtt gttgcagcca atggtcgtgt gaccttcgat caccacgtgg
    20761 gggccgacgc tcgtgccttc accgatgacc acgtgctcgc cgacgatcga atacgccccg
    20821 acctgcaccc cttcggccag gcgcgcgccc ggatgaacga tggcggtagg atggatgctc
    20881 atcgcttgat cgcgcacatg aattcggctt cggtggccag ctcgccatcg acgcgggcca
    20941 ctgccctgaa cttccagatg ttgcgcatct tgcgcacgat ctcggcttcc atgacaagct
    21001 ggtcgcccgg cactacgggc cgcttgaagc gcacgttgtc cagcgaggcg aaatacaccg
    21061 tggaatgctc gtcaggcacc gagccgtctg atttgaaggc cagcacggcc gcggtctggg
    21121 ccatggcttc gacgatcagc acgccgggca tgaccggatt ggtcggaaaa tggcccggaa
    21181 agaagggctc gttgatcgtg acgttcttga tcccgacgac ccgcttgtcc agttccagct
    21241 cgatgatgcg atccaccagc aggaaaggat agcggtgcgg cagatactgc ttgatttcgt
    21301 tgatgtcgat gacgctcatg aattttcctt gtttttttca gcgagcagct tttccagttc
    21361 ccggacccga tcggcaaggc cgtcgagacg ccgcacatgg gagacgttct tcacccagtc
    21421 ggcgtgggtc atctgcggca gcatggccgt atagacgccc ggcttgtcga gggtcttggt
    21481 aatgatggtg ccggccgata cgacgatgtc gtctcccacc gagaggtgac cggagatgcc
    21541 ggcctgccca gccaccatca cccgtttgcc gagacggctg ctcccggcga tgccggagag
    21601 cccggccatg atggcgtcgt cgtccacgtg gacgttgtgg gcaatctgga tcaggttgtc
    21661 gagcttgacc ccgttgccga tcaccgtgtc gtccagcgcg ccccggtcga tggtggtgtt
    21721 ggcgccgacc tccacgtcgt ccccgatcac cacccggccg atctggggaa tcttcaccca
    21781 gcgtttgtct gaatcccgcg cgaagccgaa accgtcggca ccgatcacgg cgccggaatg
    21841 gaggatggcg cgggcgccga cgacgcagtc ggcgtaaatc gtgacccccg cgtggagcag
    21901 gccatccggg ccgatccgca cgccatcacc gacgtgacag cccggaccga tgaccgtgtt
    21961 ctcaccgatc tcgcagccct cgccaatcac ggcattcggc cccaccgaga cggattcggg
    22021 cagcgccgac gccaccaccg cgctggcatg gatgcccgcc ggcggacgcc ggagcggatt
    22081 gaaccactgg gcggcccgcg caaagtacag atagggatcg cgcgtgacaa tgcgcgcccg
    22141 ctcggtcagt ccggcggatt tgggatcgac gatgaccgcg gacgccgtcg tgccatccag
    22201 tgctgcgcga tacttgggat tggcgaggaa ggccagatcg ccctccccgg ccttttcgag
    22261 ggtcgcgatc tggcggatca cgatggacgg atcgccgatc agttcgccgc ccagccgctc
    22321 aaccagcgca cccagggtga tctgcatcga agccaagact tacttggtct cggtgagcgc
    22381 tttgatcacc ttgtcggtga tgtcaatgcg cgggctggca tacaccgctt cctggaagat
    22441 gatgtcgaac ttctcggcct cggcgatggc cttgatggtc ttgttggccc gctccagcac
    22501 tccggcgagt tcctcgttgc ggcgctggtt cagatcctcg cggaactccc gctgcttgcg
    22561 ctggaaatcc cggttcagat cgttgaactc ccgctccttg gcacgatgtt cgccttcagc
    22621 catggtcacg ccgttctttt ccagaccttc ctgcataccc tggagctgct tggccatgcg
    22681 ttgcagatcc tgatcgcgct tttcgaattc cttttcgagc ttttgctgag ctttcttggc
    22741 cggaaccgct tccttcatga tccggtcgga attgacgaat cccactttca cttcggcagc
    22801 agccgtctgc gcaaacacag cgagaagcac acccgccagc gtggaaatac cgataccttt
    22861 cactcaaacc tccgcttgcc tgcgatgact ctttaaaaca ccgttccgag ctggaactgg
    22921 aacttctggg tccgatcctg aggctctttc ttgagcgcct gacccagact gaacttgagc
    22981 ggccccaccg gggaactcca cgaaaaggcc aaacctgcac tgtagcgcaa atccgacagg
    23041 cgaaccttct cttccacgcc ccacacttgg cccgcgtcca ggaacatcga catgcgcatg
    23101 gacttttcct tgccctggcc cggcatcggg aagtagtact ccgcgttgcc gatgatccgc
    23161 cggttgccgc cgagggcgcg tgccacgccg tatacatcca cgaactgcgg acccaacgtg
    23221 ctctgctgat acccccgcac cgagccgata ccgcccgcgt agaagttctt gtagaagggc
    23281 agttccttgc cgtcgtagcc gtgggcatag ccgacatcgc cattgagcat gagggcgtag
    23341 ccgccgccga agggaatcca gtgctgatgc tggtaactcg agcggaaata gcggatgtcc
    23401 atcggcgcgg tggccatttc caggccgaca cgctgatagg ttcccgatgt gggatagacg
    23461 aagctgtcgc gccggtcccg cgaccagccc accgtgctga ccagactttc cgcagtgctg
    23521 ccgaatttat tgacgaaatc cacgtactgg atcgggctgg tatcaaatac ggtgatcttg
    23581 gtgcgatcca ccgcgagacc aaagttgatg cggtcgtcct cggcaatcgg ataaccgaag
    23641 cgcagtccgg caccaaggga atgggtcttg tagctggcca ccgaaaggga cgtgggatcc
    23701 acattgcgct gatacacatc ccaaccgatg ctgacgccat cgatggtcca gtagggattg
    23761 gtgaaggaca gcgccgcggt acggttgatg cgacccgtat tcagctgcag ggtcatggaa
    23821 ttacccgtgc cgaacaggtt ctgctgggag atcgagcccg acaggatgac tttttcagag
    23881 ctggagaagc ccgcacccag catcaggttg cccgtggccc gctccttgac cgagaagttc
    23941 gcgtccacct ggtcggggga acccggcacc gctggcgtct cgactgtgac ttcgtcgaaa
    24001 tagccgaggc gatccagacg cgtccgcgaa cggttgagtg cagccccgtc gtaccaggca
    24061 ccttccatct gacgcatctc gcggcgcagc acctcgtcac gggtgcgggc attgccggcg
    24121 aaattgatct tgcgcacata aacacgccgc cccgggtcga catagaacgt gaaggccacc
    24181 tggcgcttga ccttgtccac ctcgggcgcc gcattcacgt tggcgaacgc atatccttcg
    24241 ttgcccaacc gatcgctgat ggccttggtg gtggccgtca cgttgtcacg cacaaaggtg
    24301 tcgccggact tgaccttgac gaggttcagg acctccttct ctggcaagac cagatcgccg
    24361 gccagcttga cgtcggacac cgtgtatttc tcaccctcgg tgatgctgag ggtgatgtag
    24421 atatccttct tgtcgggcgt gatggacacc tgggtggagt ccaccgtgaa atccagatag
    24481 ccgcggttca gatagtaggc gcgcagggcc tcgatatcgc ccgacagctt ctgcttggaa
    24541 tactggtcac tcttggaata ccaggtcatc cagcccggcg tggtcagggc gaagaggtcg
    24601 aggagatcct tctccttgaa caccttgttg ccgatgatgc tgatctggcg gatcttggcg
    24661 gcgtcgcctt cgaccacgtt gaaattcacg ccgacgcgat tgcgttcgag gggcgtgacc
    24721 gtcgtggtga tctgcacgcc gtagaggccc ttcgacagat actggcgctt caactcctgc
    24781 tcggcgcgct cgagcacggc ccggtcgaaa atccgtgact cggcgagacc gacttccttc
    24841 aaccccttct tcagggcctc cttgtcgaac tccttgatgc cgacgaaatc cacctgcgcg
    24901 atggccggcc gctcgtcgac gacgacgacg atcacgtcgc catccacctc gatgcgcaca
    24961 tccttgaaga agcccgtcgc gaacagcgcc ttgatggcct gggacgcctt gtcctcgctg
    25021 aagcgctcac ccaccttgac cggcagatag ttgaagaccg taccggcttc ggtgcgctgg
    25081 atgccttcta cacggatatc tttgacgacg aagggctcaa aggccagagc gggcgaggcg
    25141 cagatcagcg ccgcgatcac cgcggggaga agcttggaat tcatccgatt tcagctggct
    25201 acaaggcggt tgatgtcgtt gtaaaaggca aatgccatga gcatcgccag cagtgccatg
    25261 cctatctgct ggccgatctc catcgtcctg tcggaaacgg ggccgccctt gatgatctcc
    25321 agcacataat acagcaagtg ccccccgtcg agcagcggga tggggaggag gttcaggacg
    25381 cccagactga tgctgatcag ggcaaggaac ttgatgtagt ggggcaagcc catgcgcgcc
    25441 gactggccgg caaaatcggc gatggtcacg ggtccggaca ggttcttgac cgacacctca
    25501 ccgacgatca tgcggcgcat caccgacagg ctcatcacgg tcgtctccca ggtctgggac
    25561 aggccctttc ccagggcatc gacgattccg tagcgcacgg tcacaaacat ctcgtcacgc
    25621 cgctgcgcgt tttccgccaa gcccacaccg atccggccga tcgtgcgacc gcggtcctcg
    25681 acggctgccg gcacgacgtc cacggcaagc gcttccgtac cccgcaccac ctcgaatctc
    25741 aggcgacggc ccggtgcatc gcgcaccatc atgacgagtt cggaccagcc gccgatatcc
    25801 ttgtcgtcga tgcgcagcac ccgatctccg ccttgcagcc ccgcccgggc cgccacgctg
    25861 tcgggggcca cgctcccgat cacggcgggc agcaccggac ggaagagctt catgccgagc
    25921 tgggcggtga tgtcctcggt cccctgatcc acggtgattc cggcgagatc gaggcgacgg
    25981 aaggcgatct cgccctgtcc gttctgggtt tcgacctcga cagcctggtg ttcgagggcg
    26041 cggttgagca gcttccagcg gaattcctgc caggtctgaa cctgctcgcc gctcaccgca
    26101 cgcaccagtt ctccgtcctg aaagcctgcc agatcggcct gactgcccgc caccggtgtg
    26161 cccacccgcg ggcggatctc ctccactccg cctccgtaca atccccagta cagcgtgatc
    26221 gcgagcagga aattggccag cggtccggcg gcaacaatgg cgatgcgctt ccagacattc
    26281 tggcgattga aggcacggtg cagttcctcg gtcggcacct gcccttcccg ctcatcgagc
    26341 atcttcacgt aaccgcccag cggaaaggct gcgatggccc actcggtgcg atccgcgccc
    26401 gcccgccaca gcatcagcgg cttgccgaaa cccaaggaaa aacgcaacac cttgacgccg
    26461 caccagcgcg ccacctgata gtggccaaac tcgtgcacga cgatgaggat ccccagcgcc
    26521 agcaggaatg ggaggacgta gtcgaccagg atcatgggcg ggcgccagcc aggtaagccc
    26581 ggacacggga cgctgccact tcgcgagcct cacgatcggc cgcgaaaacc acctccaggg
    26641 aatcggcagc agcgtccggc acggcgtcga gcacatcggc aatcacggcg gcgatacggt
    26701 cgaatctcag atggccgtcg aggaacgcgg cgaccgccac ctcgttggcc gcattgagca
    26761 ctgccggcgc cacgccgccc gcctgcagcg cccgataggc caacgccaga cagggaaaac
    26821 gcgcgaaatc cggccgctca aaatcgagcc gggccacttc gaacagatcc agcggcttca
    26881 cgcccgaatc aatacgttcg ggatatgcca gggcatgggc gatgggcgtg cgcatgtccg
    26941 ggttgccaag ttgcgccagc acggaaccgt cggaataagc caccagggag tggatcacgc
    27001 tctggggatg gatcaccacc tggatgcgct ctgccggggc cgcaaagagc cagcgcgctt
    27061 cgatgacctc cagccccttg ttcatcatgg tggccgagtc gacggaaatc ttgcgcccca
    27121 tcacccagtt gggatgagcg caggccatgt cgggtgtcac agccggcaaa tccgagatcg
    27181 gggtggaccg gaagggcccg ccggacgcgg tgagcaaaat ctgcgtgacc cccccggttt
    27241 cgagcggccg ggcatggccg gacggcatcg cctggaagat ggcgttgtgt tcgctgtcga
    27301 tgggtagcag cgtggcgcca tgggtatcca cttcagccat gaagagtgcg ccagacatca
    27361 ccagcgcctc cttgttggcg agcagtaccc gcttgccagc ccgcgccgcc gccagggtgg
    27421 gcggcaaacc cgcggcgccg acaatggcgg ccatcacgga cgccacgtcc ggcgcagatg
    27481 ccacgtcgca cagggcagcc gggccgtata gaacgtcgca acggcactcc gccgcgtcca
    27541 ggcgccgctg caacgccacc gcatcctccg cccgttcgac cacggcgtag gcggggcgat
    27601 gggcgaggca ttgttcgtag agcttgtcca cctgccgatg ggcggtcagg gcgaatacgc
    27661 catagcggtc cggatggcgg gccaccacat cgagggtgct gacgccgatg gaacccgtcg
    27721 ccccgaggac ggtaatgagc ttcttcacgt ggatattccc tcagcgagcc agccacaggg
    27781 ccacgagggc cgccatggga agcgtcgagg tcagactgtc gatccgatca agcacccccc
    27841 cgtgaccggg cagcagggta ccgctgtcct tgatgccggc ctgacgcttg atcaaggatt
    27901 cgaagaggtc gccgatgatg ctcacggccg tgaagcccat cagaccgatc acgccaggca
    27961 tccacacccg tggggcggtc cagtcgagtc cgaccgatgc cgcgcaggca aacccataga
    28021 cgaccacgcc gacacaggcg ccgtatgccc cttcccagct cttgcccgga ctgatgctgg
    28081 gcgccagctt gcgcgatccg aaggcccgac cactgaaata cgcggcgatg tcggccaccc
    28141 aaactgccgc catcaccgcc agcagcaccc aggggccaag caggcgcaga tgggcgatcg
    28201 ccaacgaggg gggcaacaac agcaccagac ccaccagggc acccgccccc acgccgcgga
    28261 gttgccatcg ggatttcagc cagacgggca cgcaggtcag ccagaacagg gcactgaccc
    28321 cataaaccgg cgcgagtgca aagggcgcca cggtttccgg ccggtgcagt ccggccacac
    28381 atccccctgc caggcacaac gccccgagca gcacggaata gaccgccctc cgggccgccg
    28441 cgaatccgat catgccagcc cattcgtgcg cagccgccgc gcagatcagc gcacagaaca
    28501 cgacccaggc gggctcgggc agcaggaaca gtgcagccag aatgccagcc agcaggcaca
    28561 acgcggtgat gacccgcgtt ttaagcatga tcgcttgaat caggcccgcc agcgccggcg
    28621 tcaagctgct cgctcgttcg cccgaatcga cgctcacgcc cctgatagga gcggatcgcc
    28681 tccttcagcg ccgcttcgtc aaattccggc cagagcttgt cggtgaaata gagctcggtg
    28741 tacgccagtt gccacagcat gaagttgctg atgcgctgct cgcccccggt acggatgaag
    28801 aggtccggtt cgggaccgaa gctcagcgcc agatattctg caagctgatc ctccgtaaac
    28861 ccttctcggg catcgggcga agcagccagc atcttctcga cggcctgaag gatgtcccag
    28921 cgcccgccat aattggccgc gatggtgagg ttcatcgccg tattcccggc ggtcagcgtc
    28981 tcggactcct cgatgagatt cacgagcgcc gaatcgaagc gactgatgtc gcccacgacc
    29041 cgcagacgaa tgttgctgcg atgcatccgc tcgatttcct ccttgagggt acggaggaag
    29101 agcttcatga ggaaggtgac ctcgtccttc ggacgccgcc agttttctga actgaatgca
    29161 aagaccgtga ggtactcgac cttcaggtcc tggcatgtgc ggatcatcgc ccgcaaagac
    29221 tccacaccct tggcatggcc cgccacgcgg ggcatcaggc gcttgcgtgc ccagcgcccg
    29281 tttccatcca tgatgatcgc gatgtggcgc ggaacctcta cgacctcggg gatgccccga
    29341 gtggagctca gaaaaggcaa tcgcaccata atctctccgc tggacggaac gggacgcgcc
    29401 tgacccgacg gatcagatct gcatcagctc ttgttctttt tgaacaagca gcttgtcgat
    29461 ttcgacgatg gccttgtcgg tcagcttctg gatgtcgtcc tgggcacgac gctcgtcatc
    29521 ttcggaaatg gtcttggcct tgaccgcgtc cttgagcgaa ctgttggcat cacgacgcag
    29581 attgcggacc gccaccttgg caccttcgcc ttcatgcttg accaccttga tgagctcctt
    29641 acgacgctct tcggtcaagg gaggcatggg cacccgaatg atgtcaccct gggcggccgg
    29701 attcaaaccc agatcggagt cacggatggc cttttcgacc ttccccacca tcggcttttc
    29761 ccacggctgc acgccaatgg tgcgggcgtc gataagggtc acgttggcca cctggttgac
    29821 gggcaccgga ttgccgtaat actccaccat cacatgatcc agaatccccg tgtgggcgcg
    29881 gccggtacgg accttggcca gatccgcctt gagcgcctcg atggacttga gcatcttgtg
    29941 ctcggtggtt ttcttgagtt cgggaatcat cgcggcttcc tcagcaatag accagggtac
    30001 cctcgtcctc acccatcacc acacgcttga gcgcgcccac cttgaacagg ctgaacacgt
    30061 tgatgggcag acgctggtcc cggcacaggg cgaaggcggt cgcatccatg acggccagat
    30121 tgcggctgat cgcctcgtcg aaggagatgc gctggaagcg ggtggccgtc ggatccttct
    30181 tggggtccgc agtatagatg ccgtccacct tggttgcctt cagcacgatg ttggcaccga
    30241 tttcggcacc gcgcagcgcg gcggccgtat cggtcgtgaa gaacgggttg ccggtcccgg
    30301 ccccgaaaat caccacccgc ccctcttcca gataacggat ggatttgccg cgcacgtagg
    30361 gttcgaccac ctgatcgatg cgcagcgctg actgcacgcg ggcattgacg ccggcgacgc
    30421 ggaaggcgtc gccgagcgcc atggcgttca tgacggtggc cagcatcccc atgtagtcgg
    30481 cggtagcgcg gtccatgccg gacgctgccc ccgccatgcc acggaagatg ttcccgccgc
    30541 caatcacgat ccccacctcg acgccgagct ggaccacttc ggccacgtcg gtgacgatgc
    30601 ccgtcaccac gtcctggttg atgccgtagg cgtcgtcacc catcagggct tcgccggaca
    30661 gcttgaggag aatgcgcttg taagcgggct ggctcatcgt caggaacctc gcttatttct
    30721 gggcagcagc agcggcctgg gcggccactt cggcggcgaa gtcggtcacc ttcttctcga
    30781 tgccctcacc cacgatgaag agggtgaaac cggccacgga agcccccttc gccttcagca
    30841 gctgggcgat ggtctgcttg ccatcctcgg ccttgacgaa gacctggtcg agcagggtca
    30901 cgtccttgag gtacttctgg acggtgcctt cggcgatctt ttccagcatc gcttcgggct
    30961 tgccggcttc gcgggccttt tcaatggcaa tgcgacgctc cacatccagc agctcggacg
    31021 gcacgccgga cgcgtccagc gccttgggct tggccgcggc gacatgcatg gcgatgtcct
    31081 tggcaagggc ttcatcaccg cccaccagat ccaccagcac gccgatcttg gcaccaccgt
    31141 ggatgtagct ggccacggca cccttggctt cggtccgcac aaaacggcgg atgctcatat
    31201 tctcgccgat cttgccgacc agcgccttgc gcacttcctc gacggactgg ccgtcaatgg
    31261 tcagggcgga cagggcctcc acgtcagccg gattggcggc ggccaccaac tcggcgagct
    31321 tcttggtcag cgccaggaag tcgtcgttct tggccacgaa gtcggtttcg caattgactt
    31381 cgacgatggc ggccagcttg ccaccttcac cgatatacac accgacgatg ccttcggcgg
    31441 tcacgcgggc ggcggccttg gaggccttgt tgcccagctt gacgcgcagg atctcttcgg
    31501 ccttggccat gtcgccggcc gcttcggcca gcgccttctt gcattccatc atgggcgcgt
    31561 cggtcttctc gcgcagttcc ttgaccatgc ttgcggt
//
LOCUS       DAOQPA010000030        29849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_694_length_29849_cov_19.164060, whole genome shotgun sequence.
ACCESSION   DAOQPA010000030 DAOQPA010000000
VERSION     DAOQPA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29849
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_29849_cov_19.164060"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKX01_06255"
     CDS             complement(<1..1292)
                     /locus_tag="PKX01_06255"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HMZ75763.1"
                     /translation="MASTPSIATVLHVTGKAFARDTAGHLRAIRPGDSVREGEVVVTR
                     DGATVELTLANGEHLAVKPDQSVKLDPELLGSGHVDVSENAVAADSIKDVLRLLSEGK
                     DLDLDAPGAGLPGGGVGDEGHNFVRLVRILENVSPLAFAFGDPQATDVQGLAAENSTL
                     TVPAAPTVTILQDANNDGVLNAAELGTATTVAVRVGLPAGAKVGDVIVLSDGSGEQRH
                     VVTGADLTAGHWDTVVARPAEASTLSVSATLVDPAGSVSSPATDAATIYAAAPGAPTV
                     TITQDANNDGRLGSAELGTGTTVSVRVGLPADAQVGDTLVVGDGSSTQTHVLTAGDLV
                     SGFWDTNVARPASGATLAVTATLTNPNGNSSATATDSATVDTSAPAASIGLTHDAASD
                     TGASPTDNLTANPKPVLTGSAEPNSTATVTVTDSAGHTA"
     gene            complement(1420..1929)
                     /locus_tag="PKX01_06260"
     CDS             complement(1420..1929)
                     /locus_tag="PKX01_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75764.1"
                     /translation="MLWVDVCQDGGVSEALIASLHSLHPDIPIVALDAEPDAAGALRV
                     LQAGAAGYCHRLAAPEIFHQVATVVRHGGLWLRPELMGRVLGATFAVTSRPASVPAGL
                     QRLSPREREAVLAVARGGTNKEVARQLGITERTVKAHLSAAFEKLGVRDRLQLVLALG
                     RDAQLLPAA"
     gene            2242..2910
                     /locus_tag="PKX01_06265"
     CDS             2242..2910
                     /locus_tag="PKX01_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75765.1"
                     /translation="MSALLPSSPDTARPALIAPVLLLTRDAALAARWQSVSAHPQVAT
                     DTDALTGWHAAGRRLVLADLRLRDAHRVPLWESPAWRAHGSGLAVMALSMAPSDDEGL
                     AVLQAGACGYGHALLPATHLHQALEIIDKGQLWVGRALLARLLHRVDERLRPTSPPAW
                     AVPLTPREQEVARLAAQGASNSVIATTLGITERTVKAHLTAVFAKLEVADRLQLALRV
                     HGVR"
     gene            complement(2926..4845)
                     /locus_tag="PKX01_06270"
     CDS             complement(2926..4845)
                     /locus_tag="PKX01_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HMZ75766.1"
                     /translation="MSLLRQLWVSVVVAMAVVLSGAFGISLITARNYFEQQLLAQAND
                     GAVSLALSMSQQSKDRATLELLVAALFDGGHFRSIRLTDPAGQVIVERNQPDTAEGVP
                     AWLPDLLPMEARPGQALVSDGWHQLGQVTVTAARGYAYRALWKGCLQLGSLMLAIGLF
                     WAVAVSALLRWVRRPLQDMAEQAEAVGAGHFRILAEPRVIELRSMARALNHMSERVQS
                     LFAEQAGRIESLRDEATHDPVTGLFNRASFFGELRRALSDVTAPPSGTIGLLRVDDLG
                     RVNKTLGRARTDEWLAALAEALRTSQDGLEEILLARIGGADFAFLHPGGDSDAAQQTL
                     ERARAAVKALDLRLKSDEEPMRLVGAATVYRQAEAITDLMARLDLCLMQAESGDSPAH
                     ATVGDLPVPGQEGWRGALEHALAERRFALAEYPLLANDGRLIHREFMLRLTTAEGSVM
                     SAGHFMPAAVRVGLAAACDLEALRLALLRLPEGADPVAVNVAPRSLTEPGFLDRLDVL
                     LAGAGSAASRVAVEVSERGLDARLAGLEALADVLARHGAALGIEHFGRQLAALPRLYA
                     LRLSYLKLDGSFVAGLPDNEANQRLVKAIVDVAHGLHVAVYAEQVYSAEEWAAAAALG
                     VVGMTGPEASRRAGR"
     gene            complement(4898..5476)
                     /locus_tag="PKX01_06275"
     CDS             complement(4898..5476)
                     /locus_tag="PKX01_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HMZ75767.1"
                     /translation="MRLLAQQRYGAAAAEAVEVWRGSMASWRDLPEEDQLRRVNEFFN
                     RRVRFVDDVEAWGQADYWATPLEVLARGVGDCEDFVIAKYFSLRDLGVSDARLRLTYV
                     KARVGGPGSSVFLAHMVLSYYPTPDSVPLILDNLIRDIRPASSRPDLAPVYSFNSQGI
                     FVGTANQVASPVDRVSRWPELLVRMRAEGYEP"
     gene            complement(5547..5837)
                     /locus_tag="PKX01_06280"
     CDS             complement(5547..5837)
                     /locus_tag="PKX01_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75768.1"
                     /translation="MNGFYAPEYAQNAEYLFIPAPVTLLLRGTYELFTFVGRFARFLN
                     FRGLAPMFGEIQGPVLHARLISVSCHDPCALCARIPDECILRRLPSLDKRHP"
     gene            5905..7722
                     /locus_tag="PKX01_06285"
     CDS             5905..7722
                     /locus_tag="PKX01_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HMZ75769.1"
                     /translation="MFIAKTLPACLSIAFATCASTAACAAGTLPDAVEKAILRNPEVL
                     ARLHEYQAADAERDVARGGYFPRVDLQAYTGRERRDTPVTPTTTYSRPGAQLQLRQLL
                     FDGFATRNDVKRLGYARAVRYFELLGTSDDTALEASRAYLDVLRYRELSDLAKENWAV
                     HKETVDQIERRVKAGVGRRVDLEQAFGRLALAQSNWLTETSNLHDVSRRFERVVGDAP
                     DERLAPAPVFSDKLPGDKEVIAQAVRNNPGFRAAVANIRASRANVDVQRANYAPTVQL
                     LASTGMDKNLLGVTGETNTSAVQVLLNWNLLKGGSDEARIRQSHEQLYTATDLRDKAC
                     RDVRQTTAIAWNDVRKLKEQLTYLEQHQLSTEKARDAYRQQFDIGQRSLLDVLNTENE
                     LFEAKRATANARFDMTLAELRVLASAHQILPAVQLAPIAKTGPDEAGATEPADDEALR
                     CSADVPVVAPLDTAGALQGRPQPAATPLPATTAMPVVAPNAATAPLSPLEPLLKSWAA
                     AWSAKDLKAYRSFYSSQFKPDQGTPEAWLAMREKRVTKPGSIAVEVDKLSTRNLSATS
                     AEASFVQHYTSSGYSDVMNKTLQFQLENGQWKIVRESNR"
     gene            complement(7792..8895)
                     /locus_tag="PKX01_06290"
     CDS             complement(7792..8895)
                     /locus_tag="PKX01_06290"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HMZ75770.1"
                     /translation="MSDITSHAHLAPAVSQLPVSWYFDEKIFELEKRLLFDAGPGYVG
                     HALMVPEVNQFRSLEWLDHAQMLVQRDDGFYRMSNICRHRQAIMMEGSGTAERNIVCP
                     IHRWTYNPDGLLIGAPHFQNNPCLNLDRHRLENWHGLLFKGPRSANVDLGGMNVAQDL
                     DFSGYKLDRVEIHECNYNWKTFIEVYLEDYHVEPYHPGLGQFVTCDDLTWQFGEWYSV
                     QRVGITSLKKPGSMTYERWHQAVLTYYNGELPRHGAIWLTYFPNIMVEWYPHVLVVST
                     LIPTDVHKTTNVVEFYYPEEILEFERDFVEAEQAAYMETAIEDDDIGERMDRGRLALL
                     KEGRNEVGPYQSPYEDGMQHFHEFYRRIIQPHL"
     gene            9019..9252
                     /gene="xseB"
                     /locus_tag="PKX01_06295"
     CDS             9019..9252
                     /gene="xseB"
                     /locus_tag="PKX01_06295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMZ75771.1"
                     /translation="MVKTKASTPPSFETAVAELESIVQEMESGQLPLDEALTRYQRGT
                     ALLRHCQDVLDKAEQTLRTIEAGAPAAAGDSDA"
     gene            9249..10142
                     /locus_tag="PKX01_06300"
     CDS             9249..10142
                     /locus_tag="PKX01_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ75772.1"
                     /translation="MNTSFAHWMADVQARTEAALDRALPAAGIAPERLHDAMRYAVLG
                     GGKRVRPLLVHAAGLLSGATPEHLDRAACAVEMIHAYSLVHDDMPCMDNDVLRRGKPT
                     VHVQYDEATALLVGDALQTQAFLHLAEAPVCDDPMRQIGMLRLLAQASGSRGMAGGQA
                     IDLDSVGSQLSLAELEFMHIHKTGALIRAALLLGVQCGRALPETELDNLDHVGKVIGL
                     LFQVVDDILDTEADTATLGKTAGKDAAHDKPTYVSLLGVVRARELAGELCQDALDRLA
                     RFGDSAHRLCELTRFIVQRQF"
     gene            10177..12075
                     /gene="dxs"
                     /locus_tag="PKX01_06305"
     CDS             10177..12075
                     /gene="dxs"
                     /locus_tag="PKX01_06305"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HMZ75773.1"
                     /translation="MPSATSPATPRTPLLERIESPADLRKLAPAELKPLADELRSFLI
                     DSVSRTGGHLSSNLGTVELTIALHYVFNTPEDRIVWDVGHQTYAHKILTGRREAMAGL
                     RQYHGISGFPRRCESPYDTFGTAHSSTSISAALGMAEAARLRGEDRRAIAVIGDGAMS
                     AGMAFEALNNAGDIHDTNLLVILNDNEMSISPPVGALNKHLSRLLSGRIYNGARKIGK
                     RFLENLPPPVFELAKRTEEHVKGMMMPGTLFEEFGFNYIGPIDGHDLDAILPTLQNLR
                     TLKGPQFLHVITRKGQGYKLAEADPILYHGVSKFDHAAGIATGKDAKGGGKLTYTQVF
                     GDWLCDMAAQDERLVAITPAMREGSGMVRFAREFPSRFYDVGIAEQHALTFGAGLACE
                     GFRPVVAIYSTFLQRAYDQFIHDVALQNLPVMMAIDRAGLVGADGATHHGAFDLSYLA
                     CIPNLTLFCPADEDECRQMLYTAYHHNGPTAVRYPRGSGLGIAPQAAMHTLPIGKGEI
                     RREGRRTALLAFGSLLAPALQVGDALDATVANMRFVKPIDRNLILDLAARHELLVTLE
                     ENAVIGGAGSEVARVLAEAGVPVRLIQMGLPDRFVDHGDQTQLMAEVGLDAAAIYARI
                     AQVYPQNS"
     gene            12156..12962
                     /gene="folE2"
                     /locus_tag="PKX01_06310"
     CDS             12156..12962
                     /gene="folE2"
                     /locus_tag="PKX01_06310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HMZ75774.1"
                     /translation="MTVQQTPSAIPDVQNSADSRQLAINKVGIKSIRHPIKVLDKNGG
                     VQHTIAVFNMYVGLPHNFKGTHMSRFVEILNSHEREISVESFEPMLREMVKKLEAETG
                     HVEMTFPYFINKAAPVSGVQSLMDYEITFIGEIRAGGLYEFTMKVVVPVTSLCPCSKK
                     ISAYGAHNQRSHVTVTATTNDHLWIEELVQLVEGQASCELYGLLKRPDEKFVTERAYD
                     NPKFVEDMVRDVAGCLNREGRIDAYVVESENFESIHNHSAYALIEHDKRL"
     gene            complement(13056..13463)
                     /gene="rplS"
                     /locus_tag="PKX01_06315"
     CDS             complement(13056..13463)
                     /gene="rplS"
                     /locus_tag="PKX01_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMZ75775.1"
                     /translation="MSNIIQQLEQEEIARLTQGKTIPEFAPGDTVVVQVKVKEGNRER
                     LQAYEGVVIAKRNRGLNSAFIVRKISSGEGVERTFQTYSPLVASIEVKRRGDVRRAKL
                     YYLRQRSGKSARIKEKLDHKSFAAKAAAAKAQA"
     gene            complement(13551..14345)
                     /gene="trmD"
                     /locus_tag="PKX01_06320"
     CDS             complement(13551..14345)
                     /gene="trmD"
                     /locus_tag="PKX01_06320"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMZ75776.1"
                     /translation="MGSRLVTAGAGKAYDVVTLFPEMFAALTGCGITGRAQERGLYGL
                     TCWNPRDFAEDRYRTVDDRPYGGGPGMVMLPGPLEKAIAAAKARQVGLTGQAGRVIYL
                     SPQGRPLDHARVLELVAEPALVLLCGRYEGVDQRLIERCVDEEVSLGDFVLSGGELPA
                     MVLLDAMIRQLPGALNDEDSAVEDSFVDGLLDCPHYTRPVMYEGLAVPDVLLSGNHAE
                     IRRWRLQQSLGRTEVRRPDLLARRGVLSKEESRLLEAFRQARRKPA"
     gene            complement(14327..14830)
                     /gene="rimM"
                     /locus_tag="PKX01_06325"
     CDS             complement(14327..14830)
                     /gene="rimM"
                     /locus_tag="PKX01_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMZ75777.1"
                     /translation="MIVLGRIVAPYGVAGWVRVRPFGDDPLSWKKMPQWFLGTDPDGQ
                     DWAPYTLKGCRAHGKGDLVATFAEVPDRNAAEAVDGFYVAVPREALPKPDKDEYYWAD
                     LIGLEVWNTQDVRLGTVSGLLSTGAHDVLQVQDGEVERLIPFVASYVTDVDVAVRRIR
                     VEWEADW"
     gene            complement(14867..15115)
                     /gene="rpsP"
                     /locus_tag="PKX01_06330"
     CDS             complement(14867..15115)
                     /gene="rpsP"
                     /locus_tag="PKX01_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMZ75778.1"
                     /translation="MVVIRLARSGSKKRPFYNIVAADSRNRRDGRFIERIGYYNPVAS
                     GNEKGLVVNVERLSHWTGNGAQLSDTVARLVKQAAKAA"
     gene            complement(15274..16581)
                     /locus_tag="PKX01_06335"
     CDS             complement(15274..16581)
                     /locus_tag="PKX01_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HMZ75779.1"
                     /translation="MHKTLIAALLGALAAGTALATTEADVENSFNPYKGGFPKAAGLT
                     AGTVINKGNAEQFKDAIPAGVLRLLKEGLLEIKVGPTTQFELSKAYVEATRKNLGKAK
                     LGPKNGDLEGYVAGRPFPEEPDAKDTRAGEKLAWNYKYGVNWGDGAAIYPFYWKYRNM
                     QSGQVEKTIKFNFHFLNFKHRVKDAPVPEVTPNPSELFRAIYVKALDPQDLKNTQLLI
                     QRYEDDQKLDDAYLYLGFQRRVRRLATGQTTDAFLGSDLMIEDFEGYNGRISDMKWTY
                     KGTKNVILPMWNHNELKLAGDMPAEADGYKYVDFGGQGGCFHNGTWQLRKVYVLEAAP
                     VNPNHPISKRVFYMDAQLGNLNGANEIYDRKGELWKVFMVGKSHPDAHLPINKGAGIG
                     IDDAFSMVDVQSKHCTTGQFKGQVDPKLNPPSMFQVQYMRGGD"
     gene            complement(16595..18565)
                     /locus_tag="PKX01_06340"
     CDS             complement(16595..18565)
                     /locus_tag="PKX01_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HMZ75780.1"
                     /translation="MKNRSSRFQPALSLCALAVSAAFAVPVLADELPDLGGGGAEPWR
                     VDTYYENHTAGRGKDNTGRSVGLSKFRNTLQVEADKNAGNGWNIHSILRGTWDGVYRL
                     NSSEYGKDAGGPILLENTGGATVGMPTMVPQGGGLGHTAVAGAFGLTNNAFVDSYPGN
                     PNDGLRVLGDRWHAQAGGVAFGVPVRPCDYDNRGCVNFGGYGNKTLSELELPEFNSRL
                     DFIRELYARNSFTLSDGTELFVKVGKQQVVWGRTDLFRVLDVINPVDYSRNNIYDELQ
                     DIRIPMWILQTEWRMGASESMQDRNLQVVWNFDQFRANNLGQCGTPNVILDAGCFFRG
                     MKGLWDTGGTVANFAGGTLATNFGPGQIGIRNVNLPAWSFNNTQLGVKFEGITKDGLS
                     FSLNGLTYRSQLPSLRAFNSAQNPFTGVNPDPTSHLIAFDMYFPRVNLIGGSLDLQSE
                     TLGAAFRLEGALTTGEEFANTARPELYSKNRVWRSVIGMDRPTFVPWINPHRTTLFSA
                     QLFYQHIFDHELRQGAYGPVGMPDWEDNVIGTLLMKGFMHGDRVSPQVILARDFRARA
                     QTVAPSLDWLVSNDLKLTFGANLKGGSNSRNQFDDCRSCNPYNPFTAYPGQTGAGSYG
                     LGGYEPLGRFRAGPIGTAMAENEIFLMARYKF"
     gene            complement(18656..21046)
                     /locus_tag="PKX01_06345"
     CDS             complement(18656..21046)
                     /locus_tag="PKX01_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMZ75781.1"
                     /translation="MVSQFRHRITHTLHRAEEWLFANPKIVLAAILIVTALFGVALPK
                     LRIYSDFADLLPQNHPYIKVYNRLKENFGGANMIVMAIEVEKGTIFNDETLALIHKAT
                     QGIDSLPGVNHNLVGSLTHRTARKIFLDESGGFASESYYDPMKSGRTVAELEQLKKDV
                     IANPTIYGLLVSPDLKAALIKAQLVEGDIDYPKTFAALQEVRQSLATPGHALYVTGNP
                     VLTGWVYTYLNQIVEILAWTLALLTFLLVIYFRRLYGIALPLLGIALSTIWGLGFMAL
                     MGINLEPLSLPIPFLIAARATSHGVQLVVRYYEELAIVKDGRKAARNALDALFRPGSL
                     AIIVDAVGIAVLVLGAAPFNHKLGLAAGFWGFSVIFTVHFMVPLALTVLPAPKNTVNA
                     NEAVRNFLGAAMARTGGTDGGARSILLLTLIGAVGGLYFVGQVQLGEAEPGSPLLHTH
                     HDYNVSTRAINTRFPGSEELHVLMRTDEKGGIKRPEVMAAIERFQAHMLSDPTLGGAK
                     ALPGVIRVVNKLTHNDDPRWMQVPDTADEVGGLMFAYMAASPIPGALKEFVNADENEA
                     DMVFYYKDHQAETIRRVVALAEAGKRQLEAEVKGIHVELGGGIVGVTAAGNQALHTDH
                     LIIIPTVMLIAFVLVMVYYSSLHAGWLMVLPMLFATLMTYAYMGAAHIGINVNTVPVI
                     AVGVGVGIDYAVYFMDRIREEYAALHDIKQAVIRAVATTGYAVSFTAATLIAGVVMWI
                     FMSDLRFQSDAAVLLSFMLIVNAIAAVLIVPSWCVVFKPKFVTAIHYDEDGVLEAD"
     gene            complement(21056..22042)
                     /locus_tag="PKX01_06350"
     CDS             complement(21056..22042)
                     /locus_tag="PKX01_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ75782.1"
                     /translation="MKHAGKRCARTALNVALMACLALAGLTGCQSAADLSAVNAEAAR
                     PVQRYDITQALAANGRTVVAGTQSGAVLVSADQGRSWQRQMLAGASLVGLAACPDGSF
                     VGVDFFHKVWRADAAGTQWSAVPLDKPRTPLAVSCDAQGRWWVAGTAATLAVSADRGA
                     TWQVSELAEDAQLTGVQMVDARFGIATGEFGLIAVTTDGGATWSRRGKMPDEFYPYAS
                     LFVSPAEGWVSGLAGQILHTTDGGLSWTRQPNASQMPLYRLFLHEGIPYGVGAGGTVA
                     RLDGGQWQPVAYPDPLPVFLGAAASLPEQHALVIGGPGGLLRVIGTQALAAR"
     gene            complement(22164..22358)
                     /locus_tag="PKX01_06355"
                     /pseudo
     CDS             complement(22164..22358)
                     /locus_tag="PKX01_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
     gene            complement(22381..23169)
                     /gene="dmpH"
                     /locus_tag="PKX01_06360"
     CDS             complement(22381..23169)
                     /gene="dmpH"
                     /locus_tag="PKX01_06360"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HMZ75783.1"
                     /translation="MKLDQATIARLAEHLEDCELQARDTLKITDEFPDMDWEDAYAIQ
                     DAIRARKRARGTRITGLKAGLTSYAKMKQMGVETPCFGFITDYGAIPDGGEVKVSELI
                     HPKVEPEIAFVMKQALKGPGCHIGAVLAATDFVIPGMEVIDSRYRDFKFDLKSVIADN
                     TSAARYVVGGQPLPVSEVDLRTVGIVMEKNGEPVAFGAGAAVLGHPAAAIAMLANHLG
                     ARGEEIPAGALILSGGITEAVAVQAGDHVTLRVQGMGSVSLRFK"
     gene            complement(23166..24197)
                     /gene="dmpG"
                     /locus_tag="PKX01_06365"
     CDS             complement(23166..24197)
                     /gene="dmpG"
                     /locus_tag="PKX01_06365"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HMZ75784.1"
                     /translation="MNLQGKKVTVHDMTLRDGMHPKRHQMTLDQMVAIATGLDAAGVP
                     LIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPKMKQAKVSALLLPGIGTVEHLKMAR
                     DLGVHTIRVATHCTEADVSEQHITLARKLEMDTVGFLMMSHMNSPEGLVKQAKLMEGY
                     GANCIYITDSAGHLLPDGVKARLGAVREALKPETELGFHGHHNLAMGVANSIAAIEVG
                     ATRIDAAAAGLGAGAGNTPMEVFCAVADLMGIATGVDVAKITDVAEDLVFPIMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRAAVKYGVPAREILVELGRRGMVGGQEDMIEDTAIT
                     MAKARGLTV"
     gene            complement(24216..25142)
                     /locus_tag="PKX01_06370"
     CDS             complement(24216..25142)
                     /locus_tag="PKX01_06370"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HMZ75785.1"
                     /translation="MSKKIKCALIGPGNIGTDLLAKLQRSPVLEPVWMVGIDPDSDGL
                     KRAREMGLKTTADGVDGLLPHVLADGVQIAFDATSAYVHAENSRKLNALGVLMIDLTP
                     AAIGPYCVPPVNLLAHVGKGEMNVNMVTCGGQATIPMVAAVSRVQKVSYGEIVATVSS
                     KSAGPGTRKNIDEFTRTTAGAVEKVGGAEKGKAIIIINPAEPPLMMRDTVHCLVEGEP
                     KRDEITASIHAMIREVQKYVPGYRLVNGPVFDGNRVSVYLEVEGLGDYLPKYAGNLDI
                     MTAAGARTAEMFAEEILAGRLVLEPKAAASAH"
     gene            complement(25172..25966)
                     /locus_tag="PKX01_06375"
     CDS             complement(25172..25966)
                     /locus_tag="PKX01_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ75786.1"
                     /translation="MDLHLNGKRALVTGSTAGIGFATARALAQEGAEVIVNGRSRAGV
                     ADAVARLQAVVPDAKVSGVAADLSSATGCEALTATCPDVDVLVNNLGIFDPKPFESIP
                     DLEWFHFFETNVMSGVRLCRHYLPAMKARDWGRIVFISSESGVCPPAEMVHYGMSKAA
                     QISVARGLAETCTGSGVTVNSVLPGPTRTEGVATFFGRLADEQGVSVEQAERDFFAHA
                     RPTSLLKRFIEPAEVAALVAYVCSPLASATHGAALRVEGGVVRSMV"
     gene            complement(25977..26759)
                     /gene="dmpE"
                     /locus_tag="PKX01_06380"
     CDS             complement(25977..26759)
                     /gene="dmpE"
                     /locus_tag="PKX01_06380"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HMZ75787.1"
                     /translation="MDKALITQLGDELYDALTQRRTLAPLTERHPDITIEDAYQIQQQ
                     MIARRLAAGERPVGKKIGVTSKAVMNMLGVHQPDFGWLLDGMVYNEGQAIPAATLIQP
                     RAEGEIAFLMKKDVMGPGLSGADILAATEGVMACFEIVDSRIHDWKIKIQDTVADNAS
                     CGVLVLGDRIVDPRKVDLATCGMVLEKNGDIVGTGAGAAALGHPLNAMAWLANTLGRL
                     GIGLKAGDIVLSGSLAALIPVKAGDSLRVTIGGIGGCSVRFS"
     gene            complement(26769..27623)
                     /locus_tag="PKX01_06385"
     CDS             complement(26769..27623)
                     /locus_tag="PKX01_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75788.1"
                     /translation="MSTASPEIGRTLRAAGRQTNVHDQGSGAPVTLIHGSGPGVSAWA
                     NWRLVMPALAERRRVVAPDMAGFGFTERFDGESYDMQGWVGHALGVLDALEIEQTDLV
                     GNSFGGAIALALAIQHPERVRRLVLMGSVGVPFEITPALDAVWGYAPSIEAMRRLLDL
                     FAYDRGLVNDELARLRYEASVRPGFQESFGAMFPAPRQRWVDAMTSAEADIRALPNQT
                     LVIHGREDQVIPLANSLTLATWIPRAQLHVFGQCGHWTQIEHAARFARLVGDFLAEAA
                     DDEPQPLS"
     gene            complement(27638..29098)
                     /locus_tag="PKX01_06390"
     CDS             complement(27638..29098)
                     /locus_tag="PKX01_06390"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HMZ75789.1"
                     /translation="MNRIKHFINGEFVEAASGRLFDKHSPLDNRVIAQVSEAGRPEVD
                     AAVGAARAALEGPWGQITVAERVDLLYGVANEINRRFEDFLSAEVADTGKPRSLARHL
                     DIPRGAANFKIFADVVKNVPTEFFEMATPDGRGALNYAIRRPVGVVAVVCPWNLPLLL
                     MTWKVGPALACGNAVVVKPSEETPQTATLLGEVMNAVGIPRGVYNVVHGFGPGSAGEF
                     LTTHPGVNAITFTGETRTGAAIMRAAADGARPVSLEMGGKNPAIVFADADLDAAIEGT
                     LRSCFANCGQVCLGTERVYVERPLFDTFVARLKQGAESLVIGHPDSAETGMGPLISQE
                     HKQKVLGYYRKAVEEGATVVTGGGVPEVPAELKDGAWVQPTIWTGLGDDAAIAREEIF
                     GPCTLVMPFDSEDEVVRRANATTYGLAASVWTRDVTRAHRVAGRLETGLVWVNSWFLR
                     DLRTPFGGAKQSGIGREGGVHSLEFYTELKNICIKL"
     gene            complement(29131..29574)
                     /locus_tag="PKX01_06395"
     CDS             complement(29131..29574)
                     /locus_tag="PKX01_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HMZ75790.1"
                     /translation="MNATRPEPVAVTQSVISAEASAVALQAAVSRAMELGVRINVAVA
                     DSGGNLAGFLRMPGAFLQSIDIAIDKAYTAAGFGFSTKDWMKLIGHDEGMKLGFSARP
                     RLVVFGGGLPIRVGGELIGGIGVSGASEAQDEDCARAALAALGAE"
BASE COUNT         5033 a         9929 c         9885 g         5002 t
ORIGIN      
        1 gcggtgtggc ccgccgagtc ggtgacggtg acggtcgcgg tgctgttggg ctcggcgctg
       61 ccggtgagca ccggcttcgg gttggcggtg aggttgtcgg tgggagacgc gccggtgtca
      121 ctcgccgcgt cgtgggtgag gccgatgctc gcggccggcg cggaggtgtc gactgtcgca
      181 ctgtcggtcg cggtggcgga cgaattgccg ttcggattgg tcagcgtggc cgtgacggcc
      241 aaggtggcgc cgctggccgg acgggcaacg ttcgtatccc agaagcccga gacgagatcg
      301 ccggctgtca ggacatgggt ctgggtcgag cttccgtcac cgaccacaag ggtgtcgccc
      361 acctgggcgt cggcgggcag gcccacgcga acggacaccg ttgtgccggt gccgagttcg
      421 gcgctgccga gccgcccgtc attgttcgca tcctgcgtga tggtgaccgt tggagccccc
      481 ggtgccgccg catagattgt ggccgcatcg gtggccggcg aggagacact gcccgcagga
      541 tccacgaggg tggctgacac cgacagggtc gaagcttcag cggggcgcgc gaccacggtg
      601 tcccagtgcc ccgccgtcag gtcggcaccg gtgacgacgt ggcgctgttc gccgctgccg
      661 tcgcttagga cgatcacgtc gcccaccttg gccccggccg gcagcccgac acggaccgcg
      721 acggtggtgg ccgtgcccag ttctgctgcg ttcagcacgc catcgttgtt ggcgtcctgg
      781 agaatcgtca ccgtcggcgc agcgggcacc gtgagcgtgc tgttctcggc agccagcccc
      841 tgtacgtcgg tcgcctgggg atcgccgaag gcaaaggcca gcggcgagac attttccagg
      901 atgcgcacca gtcgcacgaa gttatgccct tcgtcgccga cgccgccacc cggcaggccg
      961 gctcccgggg catcgaggtc cagatccttg ccttcgctca gcagccgcag cacatccttg
     1021 atgctgtcgg ccgccacggc attttcggag acatccacgt gaccggagcc gagcaactcg
     1081 ggatccagtt tcaccgactg gtcgggcttg acagccaggt gctcgccgtt ggcaagggtc
     1141 agctccaccg tggcgccgtc acgcgtaacg accacctcgc cttcccgcac gctgtccccc
     1201 ggacggatcg ccctgagatg accggcggtg tcgcgcgcaa aggccttgcc ggtgacgtga
     1261 aggacggtgg cgatggaagg ggtcgaagcc atgacagtct ccaggagggt tgccggcgat
     1321 cggaccggca gacggaatgc gtatcgggat gcagtctagc ggtcggcgac aagcacttga
     1381 attgtcctgg tggacaatgc acgcgggccg acgcggaact caggccgcag ggagcagctg
     1441 tgcgtcgcgc cccagtgcga gcaccagttg caagcgatcg cgcaccccca gtttttcaaa
     1501 ggctgcgctg aggtgggcct tgacggtccg ttcggtaatg ccgagctggc gggcgacttc
     1561 cttgttggtc ccaccccgtg ccacggcgag caccgcctcc cgctctcgtg gcgagagacg
     1621 ctgaagcccg gccggcacgc tcgcaggacg ggacgtcacc gcaaaggtgg cgccgaggac
     1681 ccgccccatc aattccggcc gcaaccagag tccgccatgg cgcacgaccg ttgccacctg
     1741 atggaagatc tcgggcgcgg ccaggcggtg gcaatacccc gctgcgccgg cctgaaggac
     1801 ccggagcgcg cccgccgcgt ccggctcggc atcgagcgcc acaatcggaa tatccggatg
     1861 gaggctgtgc agggacgcga tgagcgcctc ggagacacca ccgtcctggc agacatcgac
     1921 ccacaacacg gccgggcggt caaacgcacg ccccgtcacc gcctcgggtg acaaggttcc
     1981 gccccccgga aaggcgctcc gccaccggtc aaggagatcc ggcctgcggc tcaccgccca
     2041 ttgattcgtc ttcacgtgat ccggcacgtc ccccccgcac ggctagaatc ggactcttcc
     2101 cggcgctgcg tcgcctccgc ggcatcgccg cggccacgcg acgtcacata aattgacgcg
     2161 ccggatcgtc ccttgatgta gaccttacgg atttgcaggc aaattacttg agtccggccc
     2221 tgcacatccc atttcacccg catgagcgcc ctcctcccat cttcccccga cactgcccgg
     2281 ccagccctga ttgcccccgt cctgctgctg acccgcgatg cagccctcgc agcacgctgg
     2341 caatccgtca gcgctcaccc gcaggtggca acggacaccg acgccctgac gggttggcat
     2401 gccgcgggtc ggcgcctggt gctggccgat ctgcgtcttc gggacgcgca ccgcgtcccc
     2461 ctgtgggaaa gcccggcatg gcgggcgcac gggtccgggt tggccgtgat ggccctgtcg
     2521 atggcgccat ccgacgacga aggcctcgcc gttctgcagg ccggcgcctg cggttatgga
     2581 cacgcgctgc tgcctgccac acacctgcat caggcgctgg agatcatcga caagggtcag
     2641 ctctgggtcg ggcgcgccct gcttgcgcgt ctgctgcatc gggtcgacga acgtctgcgc
     2701 ccgacgtcgc cgccggcatg ggccgtaccg ctcacacccc gtgaacagga ggtcgcgcgt
     2761 ctcgccgcac agggcgccag caacagcgtc attgccacga cactgggaat caccgagcgg
     2821 accgtcaagg cccatctgac agcggtgttc gccaaactgg aggttgccga ccgcctgcag
     2881 ctggctttac gtgtccacgg agtacgttga tgcgccctgc tcgcgtcagc gacccgcccg
     2941 gcgcgacgcc tccggcccgg tcatgccaac cacccccagc gccgccgctg cagcccattc
     3001 ctctgcgctg tacacctgct cggcatagac ggccacatga agtccatggg cgacatccac
     3061 aatggctttg acgaggcgct ggttcgcctc gttatcgggc agaccggcca cgaaactgcc
     3121 atccagcttg agataggaca ggcgcaaggc atacaggcgg ggcaaggcgg ccagctggcg
     3181 cccgaagtgt tcgatcccca gcgcggcccc gtgacgggcc agcacgtcgg ccaaggcctc
     3241 cagcccggcc agacgggcgt cgagcccccg ctcgctcacc tccaccgcca cgcgcgatgc
     3301 cgcgctgccg gcccccgcga gcaacacgtc caggcggtcg aggaagcccg gctccgtcaa
     3361 tgaccggggt gcgacattca cggccaccgg gtcggccccc tccgggaggc gcagcagcgc
     3421 caggcgcaag gcctccagat cgcacgccgc cgccaggccc acccgcaccg cagccggcat
     3481 gaagtggccg gcactcatca cgctcccctc cgcggtcgtc agacgcagca tgaactcccg
     3541 gtggatcaga cgtccgtcgt tggccagtaa ggggtactcc gccagggcga agcggcgttc
     3601 ggccaaggcg tgctccagcg cgccgcgcca gccttcctgc cccggaaccg ggagatcacc
     3661 cactgtcgcg tgggccgggc tatcgccact ttccgcctgc atcaggcaca gatcgaggcg
     3721 cgccatcagg tcggtgatcg cctcagcctg acggtagacc gtcgccgcgc ccacgagacg
     3781 catcggttcc tcgtcactct tcaggcgcag gtccagcgcc ttcaccgcgg cgcgagcccg
     3841 ctcgagagtc tgctgcgccg catcggaatc ccctccggga tggaggaatg cgaaatcggc
     3901 gcctccgatg cgtgccagca ggatctcctc cagcccgtcc tgggaggtgc gcaaggcctc
     3961 ggccagcgcc gcaagccatt catcggtccg ggcgcgcccg agcgtcttgt tgacccggcc
     4021 gagatcgtcc acccgcagca gcccgatggt gccggaaggc ggcgcggtga catcgctcag
     4081 cgcccgccgc aattcaccga agaagctggc gcgattgaac aggccggtca ccggatcatg
     4141 ggtcgcctcg tcacgcagtg actcgatgcg ccctgcctgt tcggcaaaca gagactggac
     4201 ccgctcggac atgtgattga gggcgcgtgc catgctgcgc agttcgatca ccctcggttc
     4261 ggccaggata cggaaatgtc cggcgccgac cgcctccgcc tgctctgcca tgtcctgcaa
     4321 cggccgtcgc acccaccgga gcagcgccga caccgccacc gcccagaaca ggccgatggc
     4381 cagcatcagg ctgcccagct ggaggcagcc cttccacagg gcgcggtagg cgtagccccg
     4441 tgcggcagtg accgtcacct gaccgagctg gtgccagccg tcgctcacca gggcctgtcc
     4501 ggggcgggcc tccatcggca gcagatccgg cagccacgcc ggcacgcctt cggcggtgtc
     4561 cggctggtta cgctccacga tcacctgacc ggcgggatcg gtgaggcgga tcgagcggaa
     4621 atggccgcca tcgaacaagg ccgccaccag cagctccagc gtcgcccggt ccttgctctg
     4681 ctgggacatg gacagggcca gggacaccgc cccgtcgttg gcctgggcaa gcagctgctg
     4741 ttcgaagtaa ttgcgcgcgg tgatcaggct gatgccgaag gcgccgctca gcaccaccgc
     4801 catcgccacc accacgctga cccacaactg tctcaagagc gacatgaccg gcctccgatc
     4861 caccatccag gcccgccctg cgcggggctc atcacgttca tggttcatat ccttccgcgc
     4921 gcatgcgcac gagcagctcc ggccagcgtg acacccgatc caccggggac gcgacctgat
     4981 tggccgttcc gacgaagatg ccctgggaat tgaaactgta taccggggcc agatccggtc
     5041 gcgaacttgc cggccggatg tcccggatca gattgtcgag aatcagcggt accgaatctg
     5101 gcgtcgggta atagctcagg accatgtggg ccaggaacac cgagctgccg ggtccgccaa
     5161 cccgcgcctt gacgtaggtc agtcgcaggc gcgcgtccga aacgcccagg tcacgaaggg
     5221 aaaaatactt ggcgatcacg aaatcctcgc aatcccccac gccccgcgcc agcacttcca
     5281 gcggcgtcgc ccagtagtcg gcctgacccc aggcctccac atcatccacg aagcgcacgc
     5341 gccggttgaa gaactcattt acccggcgca gctgatcctc ctcgggcagg tcccgccagc
     5401 tcgccatgct cccacgccag acctccacgg cctccgcggc cgcggcaccg taccgctgct
     5461 gagccagcag gcgcatgcgg tcaccgttca gcccggacca ccccgcacgt cccagcacca
     5521 ggaccattgt ggcccccagc acaccgtcac ggatgccttt tatccagcga gggtaatcgg
     5581 cgcaaaatac attcatccgg gatacgcgcg cacaatgcgc acgggtcatg acaggaaact
     5641 gaaatcagcc gcgcgtggag caccggccct tgaatctctc caaacatcgg cgcaagtccg
     5701 cgaaaattaa ggaatcgcgc aaagcggccg acaaatgtaa aaagttcata cgtacccctc
     5761 aaaagcaggg tgacaggggc aggaatgaac aaatattccg cattctgcgc atattccggc
     5821 gcatagaatc cattcatgcg gacgttgtac gttgatgcgc ctggacgcgc aaacgttcga
     5881 ttcaccggaa aaaatcacaa catcatgttc attgccaaga ctttgcccgc ctgtctgagc
     5941 atcgcattcg cgacctgcgc cagtactgct gcatgcgccg ccggcacgct gccggacgcc
     6001 gtcgaaaagg ccatcctgcg caaccccgag gtgcttgcgc ggctgcatga atatcaggcc
     6061 gccgatgcgg aaagggatgt ggcgcgcggc ggctattttc cacgggtcga tcttcaggca
     6121 tacaccgggc gcgagcgccg cgacacgccc gtcacgccga ccaccaccta cagccgtccg
     6181 ggcgcccagc tccagctgcg tcagctgctc ttcgacggtt tcgccacccg caacgacgtc
     6241 aaacgcctcg gctacgcccg cgccgtgcgc tacttcgaac tcctcggcac cagcgacgac
     6301 acggcgctgg aagcttcccg cgcctatctc gacgtcctgc gttaccgcga gctgtccgat
     6361 ctggccaagg aaaactgggc cgtccacaag gaaaccgtcg atcagatcga gcgccgcgtg
     6421 aaggccggcg tcggacgccg cgttgacctc gaacaggcct ttggccgcct cgccctggcg
     6481 caatccaact ggctcaccga aacctccaac ctgcatgatg tcagccgccg cttcgagcgc
     6541 gtggtcggcg acgcaccgga tgaacgcctg gcgccggcgc cggtgttctc cgacaagctg
     6601 ccaggcgata aggaagtgat cgcccaggcc gtccgcaaca accccggctt ccgcgccgcc
     6661 gtcgccaaca ttcgcgcctc ccgcgccaat gtggacgtcc agcgcgccaa ctacgcgccc
     6721 acggtgcagc tgctggccag caccggcatg gacaaaaatc tgctcggcgt caccggcgag
     6781 accaacacca gtgcggtgca ggtgctgctc aactggaatc tcctcaaggg cggctccgac
     6841 gaagcgcgca tccgccagtc ccacgaacag ctctacaccg ccaccgacct gcgcgacaag
     6901 gcctgccgcg acgtccgcca gacgaccgcc atcgcgtgga acgacgtacg caagctcaag
     6961 gagcagctca cctacctcga gcagcaccag ctctccaccg aaaaggcccg cgatgcctat
     7021 cgccagcagt tcgacatcgg gcagcgcagc ctgctggacg tgctcaacac tgaaaacgag
     7081 ctgttcgagg ccaaacgcgc cacggccaat gcccgcttcg acatgacgct ggccgaattg
     7141 cgcgtgctgg ccagcgccca ccagatcctg ccggcggtgc agctcgcccc catcgccaag
     7201 accggtcccg acgaggcggg cgccacggaa cccgctgacg acgaagctct gcgctgcagt
     7261 gccgatgtgc cggtagtcgc cccgctcgat accgctggcg ccctgcaggg acgcccgcaa
     7321 cccgccgcga caccgctgcc cgcaacaacg gccatgccgg ttgttgcgcc gaacgctgcg
     7381 acggctccgc tctcgccgct cgaaccactg ctcaaatcct gggccgcagc ctggtctgcc
     7441 aaggatctga aggcttatcg cagcttctac tcgagccagt tcaaaccaga ccagggtacg
     7501 cccgaggcct ggctggccat gcgggaaaag cgcgtcacca agccgggcag catcgccgtc
     7561 gaggtggaca agctcagcac ccgcaacctg tccgccacct cggccgaagc cagcttcgtc
     7621 cagcactaca catcgtccgg ctactcggac gtgatgaaca agacgctcca gttccagctg
     7681 gagaacggtc agtggaagat cgtgcgggaa tccaatcgct gagtctgcgt caccgcaaac
     7741 gtgcaaaggg agccacacgg ctccctttgc gtttcaagct gccggcggcc ttcagagatg
     7801 gggctggatg atgcggcgat agaactcgtg gaagtgctgc atcccgtcct catagggcga
     7861 ctgatacggc cccacctcgt tacggccttc cttgagcagc gccaatcgcc cccggtccat
     7921 gcgctcaccg atgtcgtcgt cctcaatggc cgtttccatg taagcggcct gctcggcttc
     7981 gacgaagtcc cgctcgaatt ccaggatctc ctcggggtaa tagaactcga ccacgttggt
     8041 ggtcttgtgc acgtccgtcg ggatcagcgt actcaccacg agcacatggg gataccactc
     8101 caccatgatg ttggggaagt aggtcagcca gatcgcgcca tgccggggca gttcaccgtt
     8161 gtaataggtg agcactgcct gatgccagcg ctcgtaggtc atggaccccg gcttcttgag
     8221 ggaggtgatg cccacccgct gcaccgagta ccactccccg aactgccagg tcagatcgtc
     8281 gcaggtgacg aactggccaa gtccgggatg gtagggctcc acgtgataat cttccagata
     8341 gacctcgatg aaggtcttcc agttgtaatt gcactcatgg atctcgacgc ggtcgagctt
     8401 atagcccgag aaatccagat cctgcgccac attcatcccg cccagatcca cgttggccga
     8461 acggggcccc ttgaacagca ggccgtgcca gttttccagc ctgtggcgat ccaggttgag
     8521 gcaggggttg ttctggaaat gcggcgcacc gatcaacaac ccgtccgggt tgtaagtcca
     8581 gcgatggatc gggcagacga tgttgcgctc agccgttccg gagccctcca tcatgatcgc
     8641 ctggcgatgg cgacagatgt tggacatgcg atagaacccg tcatcgcgct gcaccagcat
     8701 ctgcgcgtga tcgagccact ccagggaacg gaactggttc acttccggca ccatcagcgc
     8761 gtggccgacg tagccgggac cggcatcgaa gaggaggcgt ttctccaatt cgaagatttt
     8821 ttcgtcgaaa taccacgata ccggcagttg ggaaactgcc ggcgccagat gtgcatggga
     8881 agtgatgtca gacatcccga accccctttt cgtaaacgaa agcccgaaac gtaaaacgga
     8941 gtattatata gcatcaccaa tttgaccagt agggcttgtt gcgctatttt taccgtttgc
     9001 cctgagcggt cccggcccat ggtcaagact aaagcctcca ccccgccgag tttcgaaacg
     9061 gctgtcgccg agctggagtc catcgtccag gaaatggaat ccggccagtt gcccctcgat
     9121 gaagccctga cccgctacca gcgcggtacc gccctgctcc gccattgcca ggacgtcctg
     9181 gacaaggccg agcagaccct tcgcaccatc gaggccggtg cgccggccgc cgcgggtgac
     9241 tccgacgcat gaacacctct ttcgcccact ggatggccga tgtccaggcc cgcaccgaag
     9301 ccgctctcga ccgcgccctg ccggccgccg gcatcgcacc ggaacgcctt cacgatgcca
     9361 tgcgttacgc cgtactcggt ggcggcaagc gggttcgccc gctcctcgtc cacgcggcag
     9421 gcctcctgtc gggcgccacg ccggaacatc tcgaccgggc cgcctgcgcc gtcgagatga
     9481 tccacgccta ttccctcgtg catgacgaca tgccctgcat ggacaacgac gtcctgcgcc
     9541 gtggcaaacc caccgttcac gtccagtacg acgaggccac cgcactgctc gtcggcgacg
     9601 ccctccagac ccaggccttc ctgcacctgg ccgaggcacc cgtgtgtgat gacccgatgc
     9661 gccagatcgg catgctgcgc ctactcgccc aggcctcggg gtcccgcggc atggccggcg
     9721 gtcaggccat cgaccttgac tcggtgggca gtcagctcag tctggccgaa cttgaattca
     9781 tgcacatcca caagacaggc gccctgattc gggccgcact gcttctcggg gtacaatgcg
     9841 gccgcgcgtt gcccgaaacc gaactggaca acctggatca cgttggcaag gtcattggtc
     9901 tgctcttcca ggtggtcgac gacatcctcg ataccgaggc cgacaccgcc accctcggca
     9961 agacggctgg caaggatgcc gcccacgaca agccgacgta cgtgagcctg ctcggagtgg
    10021 tccgcgcgcg cgaactggcc ggcgaactgt gccaggacgc cctcgaccgg cttgcccgat
    10081 ttggcgacag cgcccatcgt ctctgcgaac tgacccgctt catcgtgcag cgtcagttct
    10141 gaaccgaatc cacgtcattt gccctcaacg cccggaatgc cctccgccac gtcgcctgcc
    10201 acgccccgaa cccccttgct cgaacgcatc gagtcgcccg ccgatctgcg caagctggct
    10261 ccggccgagc tcaagcctct ggccgacgaa ctgcgcagct tcctgattga ctcggtctcc
    10321 cgtaccggcg gtcacctctc gtccaacctg ggcacggtgg aactgacgat cgccctgcac
    10381 tacgtcttca acacgccgga ggaccgcatt gtctgggacg tgggtcacca gacctacgcc
    10441 cacaagatcc tcaccggccg ccgtgaggcc atggccggcc tgcgccagta tcacggcatc
    10501 tccggcttcc cgcggcgctg cgaaagcccc tacgacacgt ttggcacggc ccactcgtcc
    10561 acctccatct cggccgccct gggcatggcc gaggcagccc gactgcgcgg cgaggatcga
    10621 cgcgccatcg ctgtcatcgg cgatggcgcc atgtcggcag gcatggcctt cgaggccctc
    10681 aacaacgccg gtgacatcca cgacaccaat ctgctggtga tcctcaacga caacgagatg
    10741 tccatctcgc cgcccgtggg cgccctcaac aagcacctgt cgcgcctgct ctcgggacgc
    10801 atctacaacg gcgcccgcaa gatcggcaaa cgtttcctgg aaaacctgcc cccgccggtc
    10861 ttcgaactgg ccaagcgcac cgaggaacac gtcaagggca tgatgatgcc cggcaccctg
    10921 ttcgaggagt tcgggttcaa ctacatcggc cccatcgacg gtcacgacct ggacgccatc
    10981 ctccccaccc tgcagaacct gcgcaccctc aagggccccc agttcctgca cgtgatcacc
    11041 cgcaagggcc agggctacaa gctcgccgag gccgacccca tcctctacca cggcgtttcc
    11101 aagttcgacc acgccgccgg cattgccacc ggcaaggatg ccaagggcgg cggcaagctc
    11161 acctacaccc aggtcttcgg cgactggctg tgcgacatgg cggctcagga cgagcgcctc
    11221 gtcgccatca ccccggccat gcgcgaaggc tcgggcatgg tgcgcttcgc cagggaattc
    11281 ccgtcccgct tctacgacgt ggggatcgcc gagcagcacg ccctcacctt cggcgcaggc
    11341 ctcgcctgcg aaggcttccg cccggtggtc gcgatctact ccaccttcct gcagcgcgcc
    11401 tacgaccagt tcatccacga cgtcgccctg cagaacctgc ccgtaatgat ggccatcgac
    11461 cgggccgggc tggtgggcgc cgacggcgca acccaccacg gcgccttcga cctgtcctac
    11521 ctcgcctgca tccccaacct cacgctcttc tgcccggccg acgaagacga atgccggcag
    11581 atgctgtaca ccgcctacca ccacaacggt cccacggcgg tccgctaccc gcgcggcagc
    11641 ggcctgggta tcgcgccaca ggcggccatg cacaccctgc ccatcggcaa gggcgaaatc
    11701 cgccgcgaag gccgccgtac cgccctgctc gccttcggca gcctgctcgc gccagccctg
    11761 caggtcggcg acgcgctgga cgccaccgtc gccaacatgc gcttcgtcaa acccatcgac
    11821 cggaatctga tcctcgatct ggccgcccgt cacgagctgc tggtgacact ggaggaaaac
    11881 gcggtgatcg gtggtgccgg ttcggaagtg gcccgtgtgc tcgccgaagc aggtgtcccg
    11941 gtccgcctga tccagatggg cctgcccgac cgcttcgtcg atcacggcga ccagacccag
    12001 ctgatggccg aagtcgggct cgacgcggcc gccatctacg cccgcatagc gcaggtctat
    12061 ccgcagaata gttgagaaaa attgtcggga aatgatagga tcgacgctcc atggggcgtc
    12121 agatcccttt acccgtagaa acaccgagaa cagtcatgac cgtgcaacag accccttcag
    12181 cgatcccgga cgtccagaac tccgcggatt cccgccagct ggccatcaac aaggtcggca
    12241 tcaagtccat ccgtcacccc atcaaggtcc tggacaagaa cggtggcgtg cagcacacca
    12301 tcgccgtctt caacatgtac gtcggcctgc cccataactt caagggcacc cacatgtccc
    12361 gcttcgtgga gatcctcaac agccacgagc gggagatctc cgtcgaatcc ttcgagccca
    12421 tgctgcgcga gatggtcaag aagctcgaag cggaaaccgg ccacgtggag atgacctttc
    12481 cgtacttcat caacaaggcc gcgccagtct ccggcgtgca gagcctgatg gactacgaga
    12541 tcaccttcat cggcgagatc cgcgccggcg ggttgtacga attcaccatg aaggtcgtgg
    12601 taccggtcac cagcctgtgc ccctgctcca agaagatctc cgcctacggc gcccacaacc
    12661 agcgctccca cgtcaccgtc acggcgacca ccaacgacca tctgtggatc gaggaactgg
    12721 tgcagcttgt cgagggtcag gcctcctgcg aactctacgg cctgctcaag cggccggacg
    12781 aaaagttcgt caccgagcgc gcctacgaca accccaagtt cgtcgaggac atggtgcgcg
    12841 acgtggccgg atgcctcaac cgggaaggcc ggatcgatgc ctacgtggtg gaatccgaga
    12901 acttcgaatc catccacaac cactccgcct acgcgctgat cgaacacgac aagcgcctct
    12961 gagcgcgtcg cgccggcttc gacgacccgg cgcaacggcc aaaaaaaaac ggaacccgaa
    13021 ggttccgttt ttcattccgg cgaccggatc gtcgatcagg cctgcgcctt ggccgccgca
    13081 gccttggccg cgaaggactt gtgatccagc ttttccttga tgcgcgcgga cttgccggag
    13141 cgctggcgga ggtagtacag cttggcgcga cggacgtcgc cgcggcgctt cacctcgatg
    13201 ctggcgacca gcggggagta ggtctggaag gtccgttcca caccttcacc ggaggagatc
    13261 ttgcgcacga tgaaggcgga gttcaggcca cggttgcgct tcgcgatcac cacgccttcg
    13321 taggcctgga gacgctcgcg gttgccttcc ttcaccttca cctggaccac cacggtgtcg
    13381 ccgggggcga attcggggat ggtcttgccc tgagtcagac gggcgatctc ttcctgttcc
    13441 agctgctgaa tgatgttgct catgcgtcac tcctgaaaaa tggccagtgg ccttcgtgca
    13501 gagcaggtcg ccacctgatg cacggctttg ttatggaatc cggccaacga ctatgccggc
    13561 ttccgtcgag cctgccggaa agcttccagc aggcgggatt cttccttgct caacacaccg
    13621 cgccgggcca gcaggtccgg acggcgcacc tcggtccgcc cgagggactg ctgcaggcgc
    13681 cagcgccgga tctccgcgtg gttccccgac agcagcacgt caggcaccgc caggccttcg
    13741 tacatcaccg ggcgggtgta atgggggcaa tccagaagtc catccacgaa ggagtcctcg
    13801 accgccgaat cttcatcatt caatgcgccc ggcaactgcc ggatcatcgc atcgagcaac
    13861 accatcgccg gcagttcgcc gcccgaaagc acgaaatcgc ccagcgacac ctcttcatcc
    13921 acacagcgtt cgatgaggcg ctgatccacc ccctcgtaac gcccgcacaa cagtaccagg
    13981 gccggctcgg ccaccaactc cagcacccgc gcatgatcca gcggacgccc ctggggcgac
    14041 aggtagatca cccgccccgc ctgcccggtc aatccgacct gccgcgcctt cgcagcggcg
    14101 atcgcctttt ccagcggacc gggcagcatc accatgccgg ggccgccgcc atagggccgg
    14161 tcgtccaccg tgcggtaacg gtcttcggcg aaatcccgcg gattccagca cgtgagcccg
    14221 tacaagccgc gctcctgggc ccgtccggta atgccgcagc cggtcagggc ggcgaacatt
    14281 tccgggaaca gggtgacgac gtcgtaggct tttcccgcgc cggccgtcac cagtcggctt
    14341 cccactcgac acggatgcgc ctgacggcca catccacgtc ggtcacataa ctggcgacga
    14401 agggaatcag gcgttccacc tcgccgtcct gcacctgcaa cacgtcatgg gcaccggtgg
    14461 agagcaatcc gctcacggtg ccgagacgta catcctgcgt attccacact tcgaggccga
    14521 tcagatcggc ccagtaatac tcgtccttgt ccggcttcgg cagggcttcc cgcggcacgg
    14581 ccacgtagaa gccatccaca gcttcggccg cattgcgatc cggcacctcg gcgaaggtcg
    14641 ccaccagatc acccttgccg tgggcgcggc accccttgag ggtgtagggc gcccagtcct
    14701 ggccgtccgg atcggtgccc aggaaccact ggggcatctt tttccaggac agcggatcgt
    14761 ccccgaaagg acggacccgc acccagcccg caacgccata gggggcgaca atccgcccga
    14821 gcacgatcat cggcgcagcg cgagcgaggt cgaacgatca gccggctcag gcggccttgg
    14881 cggcctgctt gaccagacgg gcgacggtgt cggacagctg ggcgccgttg ccggtccagt
    14941 gggacagacg ctcgacgttc accacgaggc ccttttcgtt gccggaggcg accgggttgt
    15001 agtagccgat gcgctcgatg aagcggccat cacgacgatt gcgggaatcg gccgcgacga
    15061 tgttgtagaa aggacgcttc ttggaaccgc tgcgggcgag acgaataaca accatgactt
    15121 gttcctgatt ccgttggatg gaaaaactcg cgattctaaa tcaagacctt gccatgagca
    15181 aggtacgtac ggcgcgcagc ccatgaaaaa cggccggaag gccctgccct ccggccgcgt
    15241 cggggcggat tccgccccgt cctgggcgaa aactcaatcg ccgccgcgca tgtactggac
    15301 ctggaacatg gagggcggat tgagcttggg atcgacctgg cccttgaact gtccggtggt
    15361 gcagtgcttg gactgcacgt ccaccatcga gaaggcgtcg tcgataccga tgcccgcgcc
    15421 cttgttgatg ggcaggtggg catccgggtg ggacttgccc accatgaaaa ccttccacag
    15481 ctcacccttg cggtcgtaga tctcgttggc cccgttgaga ttgccgagct gcgcgtccat
    15541 gtagaagacg cgcttgctga tcgggtggtt gggattgacc ggcgcggcct ccagcacata
    15601 caccttgcgc agctgccagg tgccgttatg gaaacagccg ccctgcccgc cgaaatccac
    15661 gtacttgtag ccatccgcct cggccggcat gtcgccggcg agcttcagct cgttgtggtt
    15721 ccacatgggc aggatcacgt tcttggtgcc cttgtaggtc cacttcatgt ccgagatgcg
    15781 cccgttgtag ccctcgaagt cctcgatcat caggtcggag cccaggaagg cgtcggtcgt
    15841 ctgaccggtg gccaggcggc gcacgcggcg ctggaagcct aggtacaggt aggcatcgtc
    15901 cagcttctgg tcatcctcgt agcgctggat gaggagctgg gtgttcttga gatcctgcgg
    15961 atcgagggcc ttcacgtaga tcgcccggaa cagctcggac gggttgggcg tcacctccgg
    16021 caccggcgcg tccttgaccc ggtgcttgaa gttcaggaag tggaagttga acttgatggt
    16081 cttctccacc tgcccgctct gcatgttgcg gtacttccag tagaacgggt agatggccgc
    16141 gccgtcaccc cagttcacgc cgtacttgta gttccaggcg agcttctcgc cggcgcgcgt
    16201 gtccttggca tccggctcct cgggaaaggg gcggccggcc acgtagcctt ccaggtcgcc
    16261 gttcttgggt ccgagtttgg ccttgcccag gttcttgcgg gtggcctcca cgtaggcctt
    16321 ggagagctcg aactgggtgg tcggtccgac cttgatctcg agcaggcctt ccttgagcag
    16381 gcgcagcacg ccggcgggga ttgcgtcctt gaactgctcg gcgttgccct tgttgatgac
    16441 ggtgcccgca gtgagcccgg ccgctttggg gaagccgccc ttgtagggat tgaagctgtt
    16501 ctccacgtcg gcttcggtgg tggccagcgc cgtgccggcg gcaagggcgc ccagcagggc
    16561 ggcgatgagt gtcttgtgca tggtgtctcc tcgatcagaa cttgtagcgg gccatcagga
    16621 agatttcgtt ctcggccatg gcggtgccga tggggccggc gcggaagcgg cccaggggct
    16681 cgtagccgcc cagcccgtag gagccggcgc cggtctgccc gggataggcg gtaaaggggt
    16741 tgtaggggtt acaggaacgg cagtcgtcaa actggttgcg gctgttggag ccgcccttca
    16801 ggttggcgcc gaaggtgagc ttgaggtcgt tggagaccag ccagtccagg gatggggcca
    16861 cggtctgggc gcgggcgcgg aagtcgcggg ccaggatgac ctgcgggctc acccggtcgc
    16921 cgtgcatgaa gcccttcatc agcagggtgc cgatcacgtt gtcttcccag tcgggcatgc
    16981 ccaccgggcc gtaggcgccc tggcgcagtt cgtggtcgaa gatgtgctgg tagaagagct
    17041 gggcggagaa cagagtggtg cggtgcgggt tgatccaggg cacgaaggtc gggcggtcca
    17101 tgccgatcac actgcgccac acgcggttct tggaatacag ctcggggcgc gcggtgttgg
    17161 cgaactcttc gccggtggtg agggcacctt cgagccggaa ggccgcgccc agggtctcgg
    17221 actgcagatc cagggagcca ccgatcaggt tcacccgcgg gaagtacatg tcgaaggcga
    17281 tcaggtggct ggtggggtcc gggttcacgc cggtgaaggg gttctgggcg ctgttgaagg
    17341 cgcgcaggga gggcagctgg gaacggtagg tgaggccgtt cagggagaag ctcaggccgt
    17401 ccttggtgat gccctcgaac ttcacgccca gttgggtgtt gttgaagctc caggcgggca
    17461 gattaacgtt gcgaatgcca atctggccgg ggccgaagtt ggtggcgagg gtgccgccgg
    17521 caaagttggc cacggtgcca ccggtgtccc acaggccctt catgccgcgg aagaagcagc
    17581 cggcgtcgag gatcacgttg ggggtgccgc actggcccag gttgttggcg cggaactggt
    17641 cgaagttcca caccacctgc aggttgcggt cctgcatgga ttcgctggcg cccatccgcc
    17701 attcggtctg gaggatccac atggggatgc ggatgtcctg cagctcgtca tagatgttgt
    17761 tgcgcgagta gtcgacgggg ttgatcacgt cgagcacgcg gaacaggtcg gtgcgccccc
    17821 acaccacctg ctgcttgccg accttgacga agagctcggt gccgtcagag agggtgaagg
    17881 agttgcgggc gtagagctcg cggatgaagt cgagacggct gttgaactcg ggcagttcca
    17941 gttccgagag ggtcttgttg ccgtagccac cgaagttcac gcagccccgg ttgtcgtagt
    18001 cgcagggacg caccggcaca ccgaaagcga cgccaccggc ctgggcatgc cagcggtcgc
    18061 ccaagacgcg caggccgtcg ttgggattgc ccgggtagct gtccacgaag gcgttgttgg
    18121 tgaggccgaa ggcgccggcc accgcggtgt ggccgaggcc gccaccctgg ggcaccatgg
    18181 tgggcatgcc caccgtggca ccgcctgtgt tctccagcag gatggggccg ccggcgtcct
    18241 tgccgtattc actggaattg aggcgataca cgccgtccca ggtgccgcgc aggatgctgt
    18301 ggatgttcca accgttgccg gcgttcttgt cggcctccac ctgcagggtg ttgcggaact
    18361 tggacaggcc gacgctgcgc ccggtgttgt ccttgccgcg gccggcggtg tggttctcgt
    18421 aataggtgtc cacgcgccag ggttcggcgc cgccgccgcc caggtcgggc agctcgtcgg
    18481 ccagcaccgg cacggcgaag gccgccgaga cggccagggc acacaggctg agggccggct
    18541 ggaaccggct tgatctgttc ttcatgagtg tctcctcctt ggttgtacga tttgcggcca
    18601 cctgcgcggc cgccctccct tcaaatcgtg tgatctctat gcgcgcgcgt ctcctctagt
    18661 cggcttccag gactccgtcc tcgtcgtagt ggatcgccgt gacgaacttg ggcttgaaca
    18721 ccacgcacca ggagggaacg atgagcaccg ccgcgatggc gttgacgatg agcatgaagg
    18781 acagcagcac ggcggcatcg gactggaagc gcaggtcgga catgaagatc cacatgacga
    18841 cgccggcgat gagggtggcg gcggtgaagc tgacggcgta gccggtggtg gccacggcgc
    18901 ggatcacggc ctgtttgatg tcgtgcaggg cggcgtactc ttcgcggatg cggtccatga
    18961 aatacaccgc gtagtcgatg cccacgccga cgcccacggc gatgacgggc accgtgttca
    19021 cgttgatgcc gatatgggcg gcgcccatgt aggcgtaggt catcagcgtg gcgaagagca
    19081 tgggcaggac catcagccag ccggcatgca gggagctgta atagaccatc accagcacga
    19141 aggcgatgag catcacggtg gggatgatga tcaggtggtc ggtgtgcagg gcctggttgc
    19201 cggcggcggt gacgccgacg atgccgcccc ccagttccac gtggatgccc ttgacctcgg
    19261 cttcgagctg ccgtttgccc gcttcggcca gggcgacgac gcgccggatg gtctcggcct
    19321 ggtggtcctt gtagtaaaag accatgtcgg cttcgttctc gtcggcgttc acgaactcct
    19381 tcagggcgcc gggaatgggg ctggcggcca tgtaggcgaa catcaggccg cccacttcgt
    19441 cggcggtgtc gggcacctgc atccagcgcg ggtcgtcgtt gtgggtgagc ttgttcacca
    19501 cacggatgac gccgggcagg gccttggcgc cgcccagggt ggggtcggaa agcatgtggg
    19561 cctggaagcg ctcgatggcg gccatcacct cggggcgctt gatgccgccc ttctcgtcgg
    19621 tgcgcatcag cacgtggagc tcttcggaac ccgggaagcg ggtgttgatg gcccgcgtgg
    19681 agacgttgta gtcgtggtgg gtgtggagca gcggggagcc gggttcggct tcgccaagct
    19741 ggacctggcc gacgaagtac agcccgccga cggcaccgat gagagtgagc agcaggatgc
    19801 tgcgggcgcc gccgtcggtg ccgccggtgc gcgccatggc ggcaccgagg aagttgcgca
    19861 cggcctcgtt ggcgttgacc gtgttcttcg gagcgggcag cacggtgagg gccaggggaa
    19921 ccatgaagtg cacggtgaag atcaccgaga agccccagaa gccggcggcg aggccgagct
    19981 tgtggttgaa gggggctgcg ccgaggacga gcacggcaat gcccacggcg tccacgatga
    20041 tggccagcga gccgggccgg aacagggcat caagggcgtt gcgggcggcc ttccggccat
    20101 ccttcacgat ggccagctcc tcgtagtaac gcaccacgag ctgcacgccg tgggaggtgg
    20161 cgcgggcggc gatgaggaag gggatgggca gggacagggg ttcgaggttg atgcccatga
    20221 gggccatgaa acccaggccc cagatcgtgg acagggcgat gcccagcagc ggcagggcga
    20281 tgccgtagag gcgccggaaa tagatcacca gcagaaaggt gagcagcgcg agggtccagg
    20341 cgaggatctc gacgatctgg ttgaggtagg tgtacaccca gccggtgagc acggggttgc
    20401 cggtgacgta gagggcgtgg ccgggggtgg cgagggattg gcggacctcc tgcagggcgg
    20461 cgaaggtctt ggggtagtcg atgtcgcctt cgacgagctg ggccttgatg agggcggcct
    20521 tgagatccgg ggagacgagc aggccgtaga tggtggggtt ggcaatgacg tccttcttga
    20581 gctgctcgag ctcggccacg gtgcgcccgc tcttcatggg gtcgtagtag gactcgctgg
    20641 cgaagccgcc gctctcgtcc aaaaagatct tgcgggcagt gcggtgggtg aggctgccca
    20701 cgaggttgtg gttcacgccg ggcaggctgt cgatgccctg ggtggccttg tggatgaggg
    20761 cgagggtttc atcgttgaag atcgtgccct tctcgacctc gatggccatc acgatcatgt
    20821 tggcgccgcc gaagttctcc ttgaggcggt tgtagacctt gatgtagggg tggttctggg
    20881 gcagcaggtc ggcgaagtcg gagtagatgc gcagcttggg cagggcgacg ccgaacaggg
    20941 cggtgacgat gaggatggcg gcgaggacga tcttcgggtt ggcgaagagc cattcctcgg
    21001 cgcggtgcag ggtgtgggtg atgcggtgac ggaactggct gaccatgatg gggcctcagc
    21061 gagccgcgag ggcctgggtg ccgatgacgc gcagcagccc gcccgggccg ccgatgacaa
    21121 gggcgtgttg ttcgggaagg gacgcggcgg cgccgaggaa gacgggcagc ggatccggat
    21181 aggcgacggg ttgccactgg ccaccatcga ggcgggcgac ggtgccgccg gcgccgacgc
    21241 cgtagggaat gccctcgtgc aggaagaggc ggtagagggg catctggctg gcgttgggct
    21301 ggcgggtcca gctgaggccg ccgtcggtgg tgtgcaggat ctggccggcg aggccgctga
    21361 cccagccttc ggcggggctc acaaagaggc tggcgtaggg gtagaactcg tcgggcatct
    21421 tgccgcgccg gctccaggtg gcgccgccat cggtggtgac ggcaatgagg ccgaattcgc
    21481 cggtggcgat gccgaagcgg gcgtcgacca tctgcacacc cgtgagctgg gcgtcttcgg
    21541 ccagttcgct gacctgccag gtggcgcccc ggtcggcgct cacggcaagc gtggcggcgg
    21601 tgccggcgac ccaccagcgg ccctgggcat cacagctgac ggcgagcggg gtgcggggtt
    21661 tgtcgagggg gacggcgctc cattgggtgc cggcggcgtc ggcacgccag actttgtgga
    21721 aaaagtcgac gcccacgaag ctgccgtccg ggcaggcggc cagccccacc agggaggcgc
    21781 cggcgagcat ctggcgctgc cagctgcggc cctggtcggc ggagacgagc acagccccgc
    21841 tctgggtgcc ggctaccacg gtccggccgt tggccgccag ggcctgggtg atgtcgtaac
    21901 gctgcaccgg gcgcgcggct tcggcgttca cggcggaaag atcggcggcg ctctggcagc
    21961 cggtgagccc tgcaagggcc agacaggcca tcagggccac gttcagcgcg gtccgcgcac
    22021 agcgcttgcc tgcgtgtttc acctgggtct cctccagttt ttatgttttt ttgggacaaa
    22081 ccgccccctc cggcgggctc tggcccaccg gcttgatccg atccggcccg cccgctcgac
    22141 gggcgggcgc gcgggcgggg ccgctagcgg ccgaggtcct tggcgctgac gccggcgatg
    22201 ccccagttgg tcttgggcac gtcgtggatc cacacgcgga cgttctcctt gggggccccc
    22261 accgcctcca ccagggcctg ggtcaccttt tcgatcacgg cgcgcttctg ttcctcggta
    22321 cggccttcga tcatgtagat ctgcgcaaat ggcatgtcgc tctcccgttt attgggtgac
    22381 ttacttgaag cggagggaca ccgaacccat gccctgcacg cgcagggtca cgtggtcgcc
    22441 ggcctggacc gcgaccgcct cggtgatgcc gccggacagg atcagggcgc cggcggggat
    22501 ctcctcgccg cgggcgccca ggtggttggc cagcatggcg atggccgccg ccggatggcc
    22561 cagcacggcg gcgccggcgc cgaaggccac cggctcgccg ttcttttcca tgacgatgcc
    22621 gacggtgcgc agatccacct cgctcaccgg caggggctgg ccgcccacca catagcgggc
    22681 cgccgaggtg ttgtcggcga tcacgctctt caggtcgaac ttgaagtccc ggtagcggct
    22741 gtcgatgact tccatgcccg gaatcacgaa gtccgtggca gcgagcacgg cgccgatgtg
    22801 gcagccgggc cccttgaggg cctgcttcat gacgaaggcg atctccggct ccaccttggg
    22861 atggatcagc tccgacacct tcacttcgcc gccgtcaggg atcgcgccgt agtcggtgat
    22921 gaagccgaag cacggcgtct ccacgcccat ctgcttcatc ttggcgtagg aggtgagccc
    22981 cgccttgagg ccggtgatgc gggtgccgcg ggcgcgcttg cgggcgcgga tggcgtcctg
    23041 gatggcgtag gcgtcttccc agtccatgtc cgggaattcg tcggtgatct tcagcgtgtc
    23101 gcgggcctgg agctcgcagt cctccaggtg ttcggcgaga cgggcgatgg tggcctggtc
    23161 gagtttcata ccgtcagccc ccgcgccttg gccatggtga tggccgtgtc ctcgatcatg
    23221 tcttcctgac cgcccaccat gccgcggcgg cccagttcca ccaggatctc ccgcgccggc
    23281 acgccgtact tgaccgcggc acgcttggca aagagcagga aggagccata cacgccggcg
    23341 tagcccaggg tcagggcgtc ccggtcgata cggatcggga aatccatgat ggggaagacc
    23401 aggtcttcgg ccacatcggt gatcttcgcc acatccacgc cggtggcgat gcccatcagg
    23461 tcggccacgg cgcagaacac ctccatcggc gtgttgccgg ccccggcgcc caggcctgcg
    23521 gcggcggcgt cgatgcgcgt ggcgcccact tcgatggcgg caatggaatt ggccacgccc
    23581 atggccaggt tgtggtggcc gtggaagccc agctcggtct ccggcttgag ggcctcgcgc
    23641 acggcaccca ggcgggcctt gacgccgtcg ggcagcaggt ggccggcgga gtcggtgatg
    23701 tagatgcagt tggcgccgta gccttccatc agcttggcct gcttgaccag accttccggg
    23761 ctgttcatgt ggctcatcat caggaagccg acggtgtcca tctcgagctt gcgcgccaag
    23821 gtgatgtgct gctcggagac gtctgcctcg gtgcagtggg tggccacgcg gatggtgtgc
    23881 acgcccaggt cgcgggccat cttcaggtgc tcgacggtgc cgatgcccgg cagcagcagg
    23941 gccgacacct tggcctgctt catcttcgga atcacggcac ccaggtattc ctcgtccgtg
    24001 tgggcgggga aaccgtagtt caccgaggag ccgcccaggc cgtcgccgtg ggtgacttcg
    24061 atcaggggca cgccggccgc gtccaggccg gtggcgatgg cgaccatctg atcgagggtc
    24121 atctggtggc gcttggggtg catcccgtcg cgcagggtca tgtcgtggac ggtgactttc
    24181 ttgccttgga ggttcatggt gcgggtcctc gcgggttagt gggcgctggc tgcggccttg
    24241 ggttcgagca cgaggcggcc ggcgaggatc tcttcggcga acatctccgc ggtgcgggca
    24301 ccggcggcgg tcatgatgtc caggttgccg gcgtacttgg gcaggtagtc gcccagccct
    24361 tccacttcca gatagacgga gacgcggttg ccgtcgaaga ccgggccgtt caccaggcga
    24421 tagccgggca cgtacttctg cacttcgcgg atcatggcgt ggatggaggc ggtgatctcg
    24481 tcgcgcttcg gctcgccctc gacgaggcaa tgcacggtgt cgcgcatcat cagcggcggc
    24541 tcggccgggt tgatgatgat gatggccttg cccttctcgg cgccgcccac cttctcgacg
    24601 gcacccgcgg tggtgcgggt gaactcgtcg atgttcttgc gcgtgccggg gccggccgat
    24661 ttggacgaga cggtggcgac gatctcgcca tagctcactt tctgcacgcg ggaaacggcg
    24721 gcaaccatcg ggatggtggc ctggccacca caggtgacca tgttcacgtt catctcaccc
    24781 ttgccgacgt gcgccagcag gttcaccggc ggcacgcagt acgggccgat ggcggccggc
    24841 gtgaggtcga tcatgagcac gcccagcgcg ttgagcttgc ggctgttttc cgcatgcacg
    24901 taggccgagg tggcgtcgaa ggcgatctgc acgccatcgg ccagcacgtg gggcagcagg
    24961 ccgtccacgc cgtcggcggt ggtcttcagg cccatctcgc gggcgcgctt gaggccgtcg
    25021 gagtcggggt cgatacccac catccagacc ggttcgagga cggggctgcg ttgcagcttg
    25081 gccagcaggt cggtcccgat gttgccgggg ccgatcaggg cacacttgat tttcttgctc
    25141 atcgccggga ttcctttgag gtcgcgaagg gttaaaccat gctgcgcacc acgccgcctt
    25201 cgacccgcag ggccgcgccg tgggtggcag atgcaagggg actgcagacg taggcgacca
    25261 gggccgccac ttccgcgggt tcgatgaaac gcttgagcag cgaggtgggc cgcgcgtggg
    25321 cgaagaagtc ccgctcggcc tgctcgacgg acacgccctg ctcgtccgcc aggcgcccga
    25381 agaaggtcgc cacgccttcg gtgcgggtcg ggccggggag caccgaattc accgtcacgc
    25441 cggagccggt gcaggtttcc gccagcccgc gggccaccga gatctgggcc gccttggaca
    25501 tgccgtaatg gaccatctcg gccggcggac acacgccgga ttcactggag atgaagacga
    25561 tgcggcccca gtcgcgggcc ttcatggccg gcaggtagtg gcggcacaga cgcacgccgc
    25621 tcatcacgtt ggtctcgaag aaatggaacc actccaggtc gggaatggac tcgaagggct
    25681 tcgggtcgaa gatgcccagg ttattcacca gcacatccac gtccgggcag gtcgccgtga
    25741 gggcctcgca gccggtggcg gaggacaggt ccgccgccac gccgctcacc ttggcgtcgg
    25801 gcacgaccgc ctgcaggcgg gccaccgcgt cggccacacc ggcccggctg cggccgttca
    25861 cgatgacctc ggcgccctcc tgtgccaggg cgcgggcggt ggcgaagccg atgccggcgg
    25921 tggagccggt gacgagggcg cgcttgccgt tcaggtgcag atccatgttc gcgatctcag
    25981 ctgaagcgga cggagcagcc gccaatgccc ccgatggtga cgcgcaggct gtcaccggcc
    26041 ttgaccggga tcagcgcagc cagcgagccg gacagcacga tgtcaccggc cttcaggccg
    26101 atgcccaggc gcccgagggt gttggcgagc caggccatgg cgttgagggg atggccgagg
    26161 gccgccgcac cggcaccggt gccgacgatg tcaccgttct tctccagcac catgccgcag
    26221 gtggcgagat ccaccttgcg cggatcgacg atgcggtcgc ccagcaccag cacgccgcag
    26281 gaggcgttgt ccgcgacggt gtcctggatc ttgatcttcc agtcgtggat gcgggaatcg
    26341 acgatctcga agcaggccat caccccttcg gtggcagcca ggatgtcggc gccggacagg
    26401 cccggaccca tcacgtcctt cttcatcagg aaggcgattt ccccttcggc gcggggctgg
    26461 atcagggtcg cggcaggaat cgcctggccc tcgttgtaga ccatgccgtc cagcagccag
    26521 ccgaagtcgg gctggtgcac gcccagcatg ttcatcaccg ccttggaggt gacgccgatc
    26581 ttcttgccca cggggcgctc gccggcggcg aggcggcggg cgatcatctg ctgctggatc
    26641 tggtaggcgt cctcgatggt gatgtcgggg tggcgctcgg tgaggggcgc gagggtgcga
    26701 cgttgggtga gggcgtcgta gagttcgtcg ccgagttgcg tgatcagggc cttgtccatg
    26761 gcggggtctc aggaaagggg ttggggttcg tcgtccgccg cttcggccag aaagtcgccg
    26821 acgaggcggg cgaagcgggc ggcgtgttcg atctgcgtcc agtggccgca ctggccgaag
    26881 acgtggagct gggcgcgggg aatccaggtg gccagggtca gggagttggc cagggggatc
    26941 acctggtcct cgcgaccgtg gatcaccagg gtctggttgg gcagcgcgcg gatgtccgcc
    27001 tcggcgctgg tcatggcgtc cacccagcgc tggcgcgggg ccgggaacat ggcaccgaag
    27061 gattcctgga agccggggcg tacgctggcc tcgtagcgca ggcgggcgag ttcgtcgttc
    27121 accagcccgc ggtcgtaggc gaacaggtcc aggaggcggc gcatggcctc gatggacggg
    27181 gcatagcccc acaccgcgtc gagggccggg gtgatctcga agggcacgcc cacgctgccc
    27241 atcagcacca gccggcgcac ccgctcggga tgctggatgg ccagggccag ggcgatggca
    27301 ccgccgaagg agttgcccac caggtcagtc tgttcgatct ccagtgcgtc cagcacgccg
    27361 agggcgtggc cgacccagcc ctgcatgtcg taggactcgc cgtcgaagcg ttcggtgaaa
    27421 ccgaagccgg ccatgtccgg cgccaccacg cgccgtcgct cggccagggc tggcatcacc
    27481 agccgccagt tggcccacgc ggagacgccc ggcccggagc cgtggatcag cgtgacgggc
    27541 gcgccggagc cctggtcgtg gacgttggtc tggcggccgg cggcgcggag cgtgcggccg
    27601 atttccgggg aggcggtgga catggcggcg gtccgggtca cagcttgatg cagatgttct
    27661 tgagctcggt gtagaactcc agggagtgca ccccgccctc gcgaccgatg ccggactgct
    27721 tggcaccgcc gaagggggtg cgcaggtcgc gcaggaacca ggagttcacc cacaccaggc
    27781 ccgtctccag cctgccggcg acgcgatggg cgcgggtcac gtcacgggtc cataccgacg
    27841 cggccagccc gtaggtggtg gcattggcgc gacgcacgac ctcgtcctcg ctgtcgaagg
    27901 gcatcaccag ggtgcagggc ccgaagatct cctcgcgggc gatggccgcg tcgtccccca
    27961 ggccggtcca gatggtgggc tgcacccagg cgccgtcctt cagctcggcc ggcacctcgg
    28021 gcaccccgcc gccggtgacg acggtggcgc cctcctccac cgccttgcgg tagtagccga
    28081 gcaccttctg cttgtgctcc tgggagatca gcgggcccat gccggtttcg gcgctgtccg
    28141 ggtggccgat caccagggac tcggcgccct gcttgaggcg cgccacgaag gtgtcgaaga
    28201 gcgggcgctc cacataaacc cgctcggtgc ccaggcacac ctggccgcag ttggcgaagc
    28261 aggaacgcag ggtgccttcg atggcggcgt ccaggtccgc gtcggcgaag acgatggccg
    28321 gattcttgcc gcccatctcc agggacaccg gccgcgcgcc gtccgccgcc gcgcgcatga
    28381 tggcggcgcc ggtgcgggtc tcaccggtga aggtgatggc attgacgccg ggatgggtgg
    28441 tcaggaattc gcccgcggat ccgggaccaa agccgtggac cacgttgtac acgcccctgg
    28501 ggatgcccac ggcgttcatc acttcgccca gcagcgtggc ggtctggggc gtctcctcgg
    28561 aaggcttgac caccaccgcg ttgccacagg ccagggccgg accgaccttc caggtcatca
    28621 gcagcagggg caggttccac ggacacacca ccgccaccac gcccaccggc cggcggatcg
    28681 cgtagttcag cgcgccgcgg ccgtccgggg tggccatctc gaagaactcc gtggggacgt
    28741 tcttcaccac gtcggcaaag atcttgaagt tggcggcgcc gcggggaatg tccaggtgac
    28801 gggcgaggct gcgcggcttg ccggtgtcag ccacctcggc ggacaggaag tcttcgaagc
    28861 ggcggttgat ctcgttggcc acgccgtaga gcaggtcgac ccgctccgcc acggtgatct
    28921 ggccccacgg accttccagg gcggcgcggg cggcaccgac ggcggcgtcc acctcgggcc
    28981 ggccggcttc gctgacctgg gcgatgacgc ggttgtccag gggcgaatgc ttgtcgaaca
    29041 ggcgcccgct ggcggcctcg acgaattcgc cgttgatgaa gtgcttgatc cggttcatct
    29101 gttgttcttc cttcgtgggt gaagtgctgt tcattcggcg ccgagggccg cgagggcggc
    29161 ccgggcgcag tcttcgtcct gggcttccga ggcaccggac accccgatgc cgccgatcag
    29221 ctcgccgccg acgcggatcg gcagcccgcc gccgaacacc accaggcgcg gacgggccga
    29281 gaagccgagc ttcatgcctt cgtcgtggcc gatcagcttc atccagtcct tggtggaaaa
    29341 gccgaagccg gcggccgtgt aggccttgtc gatggcgatg tcgatggatt gcaggaaggc
    29401 gccgggcatg cgcaggaagc ccgccaggtt gccgccgctg tccgccaccg ccacgttgat
    29461 ccgtacgccc agttccatcg cccgggacac ggccgcctgc agggcgacgg cgctcgcttc
    29521 ggctgagatt acggactggg tcacggcgac gggttcgggg cgggtggcgt tcatggtcgg
    29581 gtttcggcgt cttgttggtt aaacgatatt tctattattt ctcgacgtca actgtttttc
    29641 tcgagatttt gaattattct tgtgggaaac ggtttgggat aaaattcacc ggccccaggt
    29701 tcgccccttc cggccggaca cacaccaagg cgagagacca tgagcgaaca tcccagcatc
    29761 caggacacgg gcagcagccc cgacgacgaa cccaagaccc tggccgaggc ggcctaccgg
    29821 caactgcgcc gggacatcat cgaaggcgt
//
LOCUS       DAOQPA010000031        29697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_697_length_29697_cov_18.259126, whole genome shotgun sequence.
ACCESSION   DAOQPA010000031 DAOQPA010000000
VERSION     DAOQPA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29697
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_29697_cov_18.259126"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1751)
                     /gene="rmuC"
                     /locus_tag="PKX01_06400"
     CDS             complement(378..1751)
                     /gene="rmuC"
                     /locus_tag="PKX01_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HMZ75791.1"
                     /translation="MMPDTASTQILPWLLAALGLSLVLLVWLAFQVAALARRQDAVAQ
                     DLADSLADRVEHLLGHQQLQFVKELQAATSAATDRVTDLLAEQGERLQARLATQREET
                     ALRLAELGAGVAQGQERLRSELLAETLRTLSEHARADRELLQSGLQAASAQLTHGIEG
                     LGRTVGERLDAIGGHVNQRLDEGFRKTNETFVNVMARLATIDEAQKKIGDLTTNVVSL
                     QELLGDKRARGAFGEVQLEALVRNALPPDAFDFQYTLPNSTRADCVLRLPAPTGLVVV
                     DSKFPLENYHRMFAADQGDADRRTAQAAFRADVKKHVDAIASKYILPGVTSDGAVMFV
                     PAEAVFAEIHAYHPEVVTHALAKRVWIVSPTTLMAVLNTARAVLKDVETRRQIHVIQD
                     ALGKLAKDFARFDERMQKLATHIRQAGEDVADVQTSSRKITAHFQKIEAARLDDLPPA
                     LPELDGD"
     gene            complement(1827..4322)
                     /locus_tag="PKX01_06405"
     CDS             complement(1827..4322)
                     /locus_tag="PKX01_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ75792.1"
                     /translation="MTVRQRLALSFRMLRRDLRAGELGLLLVALVIAVASLTSVGFFT
                     DRVGRGLNRQASQLLGGDLLIAADHPLAPDFRAEAARRGLASVESTTFTSMASLGDAT
                     QLVGVKSVEAGHPLRGEIRIAPGLNQPDAPARGIPAPGEVWLDERSATALGAHPGDLV
                     GLGRSRLRMAAVLSFEADRGANFFALLPRIVMNAADLPATGLIVPGSRVTYRLHLAGS
                     IAAVAGYEAWAKARLGRGESLESVDNARPEIRATLDRTERFLRFAAMLAVVLAAVAVG
                     LATRRFVERHLDGCAVMRCLGARQTTLLGLFIGEFTLLGLAGGLAGSALGFGVQYALE
                     RFLGSLVSFELPPPSVWPLAHGLAVSLVLLLGFALPPLIRLGRVPTLRVLRRELGGVA
                     VRDGLAWSLGAAVLTGLLMLVARDVTLGAWVTGGFVAAFGVFAGVGWIVLRGVGRIRA
                     GAGSGWRYGLAALRRRPGASLVQVLALGMGLTALLLLTLVRGDLLTSWQQATPPDAPN
                     RFIIGIQPDQLDPLRDAFRAAGRPAPVLLPMIRGRLVAVNGRPVGPDDYTDDRARRLV
                     DRDFNLSHDAALPPGNRVIAGTWHGHDTTPRFSVEQGLAQTLGLRMGDVLRFNVAGET
                     VEAPIASLRKLDWDSMRVNFFVIASPGLLADFPASYITSFYLPPAQTDFVPRLVARFP
                     NFTVIDVAAMLAQVQGMVDQLVAAVQFVFGFAVVAGVVVLLGALQSTHDERERELAIL
                     RTLGARNAQLRAALLAEFAVLGVLSGSLAGVAASGIGWSLGRFVFTLPYTPSLLPVLV
                     GALAGGLAVTLAGWFGTRRLLLTPPLASLRALG"
     gene            complement(4556..5419)
                     /locus_tag="PKX01_06410"
     CDS             complement(4556..5419)
                     /locus_tag="PKX01_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ75793.1"
                     /translation="MKRIPSQTALTFALLIGSVAGAHAATAWTLTSGGTETGAGNFGN
                     TRTYGSTGSTVTISAWSDTKNSTTAGNAYIESAYLGTYSGGLGVTNRDGATGAGDTNE
                     GTIANTTSPGHTMDNNARYDSMLFDFGAGNAVKLQSVTVGWWYSDSDLTVLAYTGTGT
                     PTFTSANQGYASLLSSGWTTVKSSAGTTGTANYADAAGGAPSQSSNSAGYIPLAVNDL
                     NVSARYWLIGALNTLVQALPNGATLGSGNDYIKIAAVTGTYTKVPEPSSLALLGGTSL
                     IALLGMRRRKN"
     gene            complement(5582..8401)
                     /gene="prsT"
                     /locus_tag="PKX01_06415"
     CDS             complement(5582..8401)
                     /gene="prsT"
                     /locus_tag="PKX01_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HMZ75794.1"
                     /translation="MPTSQQPHVTLRRLPAALLCLCLTLPGWAAGASDSATRYYEDAA
                     ARLARNDAAGAIIQAKNALQRDPEQLPAHLLLARALLKDGQAAAAEAEFEAALRLGVN
                     PSEIALEYGQALMMFGNSEKLLERIKADGLPLRLRAEVLAMRATAQADRGDMRAAVAE
                     VKLAREADPRALSPLLAEIDLALRSRDSARARQVLDTALGIAPGDARLLHLQAGLLQG
                     AGDARGAMEAFNRAIAADPRMLDALVGRASLLIDLKRANEALPDLERAAALVPREPRV
                     AYLKSLVYAQRGDTQASRAQLEDVVRLVDELPQEFVGRQPTLLMIGGLASQALGRGER
                     ARALLELYVRRMPGDAAGRKMLANIYLAAGETARVGDLLQPLISSGDADPQALTTLAA
                     LRMQQRRYRDAADALEAAARLTGNPGITAQMGFARIANMQPDIGLSALRSAFDKEPGQ
                     LNVATALSTLYLRRGDLRNALAVAEATTRRLPSDPAAHNLLGAVKGAARKPAEARAEY
                     TQAITLAPGFLPAQLNLARLDLVEGRADAARQRLAALLKAHPADVQVLTENARLELGA
                     GRSADALALLEKAYALKPADPTVAQELLALYQKLGKPAPAVAVAKQLALSRPDDPLVL
                     ETLGRAHLAANNLADARSTFNTLSRLPGLEPGQLVAVGRLQLDAGAFREASASAEKAL
                     ARNAGLVPAQILQIESEMALGDLNRADALLKSFATRSGNSAEALRLAGDLARARQQPR
                     DAARLYRSAFDKAPSLALVLRGVEASFQAGEGDRAVSLLDTWLRTHPNDLTARSALAE
                     SHFRLGHLADARRLYEGVLVAEPANANVANNLAQILMALNDPGALNAAEAAYRLAPGD
                     ANVLDTVGWARARSGALDIGLKALREARLRAPESREIRYHLAWVLLRKGLRDEARAEL
                     QGALNSTNGSGYDIEIQNLRRELGV"
     gene            complement(8665..9834)
                     /locus_tag="PKX01_06420"
     CDS             complement(8665..9834)
                     /locus_tag="PKX01_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HMZ75795.1"
                     /translation="MHLLDSLRADHADITALRRDIHAHPELAFEEHRTAGLVAERLRA
                     LGIETHTGIGKTGVVGVLRAGSGRRAIGLRADMDALPIQEKNDFAHHSRHPGRMHACG
                     HDGHTAMLLGAAAHLSRHPEFDGTVYFLFQPAEEGEGGAPEMIADGLFERFPMDAVFG
                     LHNWPGLPLGEIAVHRGAVMACADRFDIEIRGHGAHAAMPHQGIDPVLAGAALVQALQ
                     SVVSRGTDPQDAVVLSVTQFQAGDAYNVIPDVARIGGTVRAFRPELQARVEDAMQRIC
                     AGVGAAFGANVHFEYRRGYPPTINSVAEAEFCARVAAEVCGDARVSVDPRPSMGAEDF
                     SYFLQEKPGCYIWLGNGPGEGGCTLHNPHYDFNDAAIPYGVAYWVRLVQRWLADA"
     gene            9905..10825
                     /locus_tag="PKX01_06425"
     CDS             9905..10825
                     /locus_tag="PKX01_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMZ75796.1"
                     /translation="MKLFYADHFVLPLPPGHRFPMEKYARLRERLQASGLFRPDDFHE
                     PPPATDTEILRAHDAGYLQRVVRGQLTPDELRLIGFPWSPAMVERSRRSSGATLAACR
                     AALEDGCAVNLAGGTHHAFRDRGEGFCVFNDAAIAALAMRAEHRVERLAIVDCDVHQG
                     NGTAAILAHRADCFTCSLHGAKNYPFDKERSDLDVELPDGTADGPYLDALAMALETVF
                     ARADPQLVIYLAGADPYADDRLGRLALSLDGLAARDRLVLGECRRRGVSVAIAMAGGY
                     ARQIDDTVAIHARTVTLARQRFAPGPTEAP"
     gene            10822..11664
                     /locus_tag="PKX01_06430"
     CDS             10822..11664
                     /locus_tag="PKX01_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMZ75797.1"
                     /translation="MSQESHIALLIDADNCPAAKIEEILDELAKYGVINIRRAYGNWK
                     SPGLKSWEAVLHEYAIQPMQQFAYTKGKNATDSAMIIDAMDLLYSQNLDGFCLASSDS
                     DFTPLVMRIRANGLKVFGFGEKKTPSPFVNACSKFLYLENLGATDADTPASEAPPAAV
                     PSARAESDGGAGRGTAAGDAGKAAESSTRPVARPTPRELRGDARLVNLLRKSVEAAAD
                     DDGWAALGEVGQHISKQASFDSRNVGYARLRDLMQDIGLFDVSQRDKAIYVRDKRLAR
                     KPAP"
     gene            11732..12574
                     /locus_tag="PKX01_06435"
     CDS             11732..12574
                     /locus_tag="PKX01_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ75798.1"
                     /translation="MRFEGTETYVATPDLMLAVNAAVTLQRPLLIKGEPGTGKTLLAE
                     EVAAALGMPLVQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIGQYIVRGPLWQ
                     AFESDVPCVVLIDEIDKADIEFPNDLLRELDRMEFYVYETRQLVKARHRPIVFITSNN
                     EKELPDAFLRRCFFHYIKFPDRDTMQKIVDVHFPGLKPALIQEALQVFFGLREVPGLK
                     KKPSTSELIDWLKLLVAEDIPPEALHSTDRKVIVPPLHGALLKNEQDMHLFERLVFMA
                     QRNR"
     gene            12585..13763
                     /locus_tag="PKX01_06440"
     CDS             12585..13763
                     /locus_tag="PKX01_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ75799.1"
                     /translation="MLIDFFLHLRERKLPVSTKEFLTLLEALQAGLARHSLDDFYFLA
                     RSCLVKDEAHFDRFDQAFGEYFKGVQAIPGLDVDLPDEWLKATMKRHLSPADKAKLEK
                     LGWDTLMAEFKKRLQEQRERHAGGSKWVGTGGTSPFGNSGYHPEGIRVGGESAGNRTA
                     VKVWDRREYRNLDDSVELGTRNIKVALRRLRRFARLGAPDELDLDGTIAATARNAGYL
                     DLLMRPERHNAVKVLIFFDVGGSMDPHVKVCEELFSACRAEFKHLEYFYFHNCVYDHV
                     WRDSTRRYTERTPLADVIHKYGPDYKLIFVGDATMSPYEILQPNGSIEYASSEAGAVC
                     LRRLADAYPALAWLNPEPERTWEFRQSIGIIRELLGERMFPLTLDGLTRAFAHLSRKH
                     "
     gene            complement(13830..14600)
                     /locus_tag="PKX01_06445"
     CDS             complement(13830..14600)
                     /locus_tag="PKX01_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ75800.1"
                     /translation="MAVELFNDGKHACLAFYDLVDEEADHAVQCNQFLIVDGEHGALV
                     DPGGNMTYNGLLMAMQRFFPSRGLDYIFATHADPDIIASLNKWMVTTHCRVMISRLWA
                     RFVPHFTTGKDYTSRITGIPDEGMNIALGDSRIKALPAHFMHSEGNFQFYDPVAKILF
                     SGDMGTSLVDHAQAAEPVRNFDDHVITMAGFHRRYMISNKICRYWVHMVRQLDVDMIV
                     PQHGCRFEGKAMVNRFLDWIENLPCGIDLMTQDNYRAP"
     gene            14886..15425
                     /locus_tag="PKX01_06450"
     CDS             14886..15425
                     /locus_tag="PKX01_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75801.1"
                     /translation="MGMSSGFGSSGMGSANTLSGGIGTRSLLDSKQNGPQIKDLRRPS
                     ASECAMRELAGKKSPGMTANGQAGSGKVTTYTDASSLRGRSGDIYGDCDGAQGMRRRG
                     AGGSTGTSSFVGIGSATRPQTAYDRRTGTLKAAKDGYVNRGATSSMMPKHPESAYDPV
                     TGTLKPPRVQSAYQIYQQQ"
     gene            complement(15526..17445)
                     /gene="htpG"
                     /locus_tag="PKX01_06455"
     CDS             complement(15526..17445)
                     /gene="htpG"
                     /locus_tag="PKX01_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMZ75802.1"
                     /translation="MAVEQMSAQTMNFQAEVKQLLHLMIHSLYSNREIFLRELVSNAS
                     DACDKLRFEALENGALLAEDAELKIRVAYDKAARTLTIADNGIGMSRDEVVTHLGTIA
                     KSGTKEFFGKLTGDQQKDAHLIGQFGVGFYSAFIVADTVTVRTRRAGLSAGDAVKWEC
                     TMTGDTAGQYTVEPIEKASRGTEITLHLREDQDDLLSAWKLQSIIRKYSDHILQPIVM
                     KKESWDEDKKEQVVTDEDETVNKANALWARPKSDVSDEEYTEFYKHVGHDYEPPLAWT
                     HSRVEGRHEYTQLLYVPGHAPFDMWDRQARHGVKLYVRRVFIMDDAEKLMPLYLRFVR
                     GVVDSNDLPLNVSREILQESKDIDTLRAGCTKKVLGLLEDLAENQKDKYTTFWKEFGQ
                     VLKEGVGEDFSNKEKIAGLLRFASTQADTADETVSLADYIGRMKEGQDKIYYVTADSF
                     NAAKNSPHLEIFRKKGIEVLLLSNRVDEWVVGHLTEFDGKALASVAKGGLDLGTLEDE
                     AEKAAVEKETGEFKELLDKMKGSLGDKVKDVRITHRLTDSPACLVADEHDMGMNLARL
                     MKAAGQSMPMSRPILEINPAHPVVLRLKYETGQFDDWAAVLFDQALLAEGGTLDDPAS
                     FVKRINQLMLAMNAG"
     gene            17525..19327
                     /locus_tag="PKX01_06460"
     CDS             17525..19327
                     /locus_tag="PKX01_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75803.1"
                     /translation="MEPSHASPSGFAESDRPADVLPAGTSKGRRFSRQRIAIWGGFAL
                     LAALVAAVAVSLLRYDRAQALHRAEAELRGLTRVLEEHVARTFGETDSALSDLGQQLS
                     GRLGSDAPSERDLNALLRQRAAHLPQVEYLYVEREDGSLLADTFDLPRGAEAEAPKRG
                     LPTLHDGLEIAAPVRSAHSGHWVTPVKRQIYDAAGKFVGVVGAAMSQDYFNGVYRDLQ
                     IFPDDAILIVDATRGTLQLRHPPTDGQVGAPLGPSSALGLDANRRSAVSIEGDETPAA
                     RLTAFRRLADYPLVVGASRPLERVLAGFSDNYRRISAGAALLIALLGVLALLLHRDSL
                     RRDGDRQSLAEVNANLEERVRTRTAELEQSNRELVSFSYSISHDLRAPLRAINGYAHL
                     LDEEYGDRLDEGGHAYLARLRKASLRMGELIDELLKLANVSRQPLHIELTDVSTMARE
                     ILDEFAAQSSGRVVHIHVEDGLATEADPNLLRTALQNLLGNAWKFTRDSQPALIQVGG
                     RPHGQERLFYVTDNGVGFDLAHAGKLFEPFQQLHRNAGFEGSGIGLASVRRIVERHGG
                     SVWAESAPGAGTTIFFTLPRSPAIVRRPRERVQV"
     gene            complement(19340..19891)
                     /gene="greB"
                     /locus_tag="PKX01_06465"
     CDS             complement(19340..19891)
                     /gene="greB"
                     /locus_tag="PKX01_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HMZ75804.1"
                     /translation="MNKAFVKESDDDEEDAPAGAPSLPPGTRNYMTRNGFERLRAELD
                     QLVRVERPALVETVRWAASNGDRSENGDYIYGKKRLREIDRRVRFLVKRLETATVVTP
                     SEQENTEQVFFGATVTVFDASADAEQTYQIVGVDEANASEGRISWIAPLSRALLKARE
                     GDVVRFASPAGVREIEVVEIRYE"
     gene            20021..20701
                     /locus_tag="PKX01_06470"
     CDS             20021..20701
                     /locus_tag="PKX01_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ75805.1"
                     /translation="MHPILSGKRVLLTQADCFMGPALQRVFEAAGAAVIADDRPLLDP
                     ALPEALVRQAGDIDILLAHLAVPSPRTPATDVADDEWREVFAHLVDPLPRLARAVLPG
                     MVARGSGRILVLGSASALRGMRNASTYSAARGAQLAWVQALGVEVARSGVRVNAIAQN
                     FVENPTYFPPEVQANPAFQERLAREVPLGRLVSADEDARFAAYLCSDDADCFVGQVFP
                     VCGGWAVR"
     gene            20726..21784
                     /locus_tag="PKX01_06475"
     CDS             20726..21784
                     /locus_tag="PKX01_06475"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HMZ75806.1"
                     /translation="MRLQELLSIEWPLIQAPMAGVQGSALAMAVSQAGALGSLPCAML
                     TPDALQAELAVLSASTARAYNVNFFAHTPPQPDGAREAAWRTQLSPYYRTFGIDPAGI
                     ATGPGRMPFSAAIADLVEPFRPPVVSFHFGLPDAALLARVKAWGARVLSSATTVEEAR
                     WLAARGVDGIIAQGLEAGGHRGHFLDHDLTVQSGTFALLPQIVRAVQVPVIAAGGIAD
                     ADGVAAAMALGAAGVQVGTAYLLCPEATTSPIHRAVLRTESARHTALTNLFSGRPARG
                     IVNRLMRELGPLNPEAPEFPLATAAVAPLRAAAEALGSGDFSPLWCGENASGCREIPA
                     AELTRALIQGFQRRETAC"
     gene            21805..22062
                     /locus_tag="PKX01_06480"
     CDS             21805..22062
                     /locus_tag="PKX01_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ75807.1"
                     /translation="MPCREACGACCIAPSISSPIPGMPEGKPAGVRCVQLDAADRCRI
                     FGQAERPAVCGSLRPTQSLCRGSREEALQALAWLEAHTAPG"
     gene            22216..22944
                     /locus_tag="PKX01_06485"
     CDS             22216..22944
                     /locus_tag="PKX01_06485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ75808.1"
                     /translation="MPPPQTPAATLIVPGYLGSGPAHWQSWIESRIPDTRRVRHVDWD
                     APVLARWAQAVRDEIDAAAGPLWLVAHSFGCLASVVAAADRPDKVAGLMLVAPADPER
                     FTPRGVRTSTGGVTEPGLSGCLPRRLLPMPGLVVASTNDPWVRLSSAAYWADQWGCRL
                     AEIGAAGHINADSGHGPWPLGLRLFESLRRAAGDWPLGRIDGNAQPRGGRHGALARVR
                     QLTRLASGRETGGGAPSEPSGRGG"
     gene            complement(22959..23903)
                     /locus_tag="PKX01_06490"
     CDS             complement(22959..23903)
                     /locus_tag="PKX01_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HMZ75809.1"
                     /translation="MNFQQLRIIHETVRRNFNLTEVANALFTSQSGVSKHIKDLEDEL
                     GLELFVRKGKRLLGLTEPGRELSAVVDRMLMDARNIKSIAEQFSNKDHGQLTVATTHT
                     QARYALPRVVTRFKQAYPRVHLMLHQASPGEIAAMLLDGRADIGIATEALAGVSDFVS
                     FPYYGWHHAVIVPAGHALEAVTPLTLEAVAEHPLITYHEGFTGRAKIDETFAQAGFTP
                     DIVMSALDADVIKTYVELGLGIGIVASMAYDAERDRALRMLPSDHLFGENVTRIAVRR
                     GHFLRGFAYRFIELCAPELSEAVVRGALRPETGSDGPF"
     gene            complement(23903..25006)
                     /locus_tag="PKX01_06495"
     CDS             complement(23903..25006)
                     /locus_tag="PKX01_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HMZ75810.1"
                     /translation="MSIQVRNIHKQFGSFTALHDVSLDFPTGQLVALLGPSGCGKTTL
                     LRIIAGLEAADSGQVLLDGQDASHTHVRERQVGFVFQHYALFRHMTVFDNVAFGLRMK
                     PRGQRPSDATIRAKVKELLGLVQLDWVADRFPSQLSGGQRQRIALARALAVEPRVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHITSLFVTHDQEEALEVADRVVLMDHGKVEQV
                     GTPDEVYRHPASPFVYGFLGAVNLFHGRVDGQAVHVGDDLLSRQRVELGQGAEVLAFA
                     RPHELTIVVDPAAADGVPARVSRILAFGAVARVELDGLNGAGGQLYEVDLPRDDVHRL
                     GLEEGQVVRLQPSRLKVFERQEAAAAAAPNWVI"
     gene            complement(25008..25895)
                     /gene="cysW"
                     /locus_tag="PKX01_06500"
     CDS             complement(25008..25895)
                     /gene="cysW"
                     /locus_tag="PKX01_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HMZ75811.1"
                     /translation="MSGAAAALHGVFDHAARFESRSATREPAWVKWSLIGLALAFFAV
                     FLLLPLVAVFYEALRKGWAVYYAALIEPDAVSAITLTLTAALCAVPLNLVFGVAAAWA
                     ITKFEFRGKHLLITLIDLPFSVSPVVSGLIYVLVFGAQGWFGPWLSDHDVKVIFAIPG
                     IVLATVFVTFPFVARELIPLMEAQGREEEEAAVVLGARGWQTFWHVTLPNVKWGLLYG
                     VILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQFAAAFAVASLLALLALV
                     TLVIKTWVERQAGRANKEE"
     gene            complement(25895..26731)
                     /gene="cysT"
                     /locus_tag="PKX01_06505"
     CDS             complement(25895..26731)
                     /gene="cysT"
                     /locus_tag="PKX01_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HMZ75812.1"
                     /translation="MSAARSLRVLPGFGLSLGYTVAYLSLIVLIPLAAVFLKAGSLGF
                     EHFWQAATSPRVLASYRLSFGASLIAALINAVFGLLLAWVLVRYAFPGKRLVDALVDL
                     PFALPTAVAGIALTALYAKNGWVGQILEPWGIQVAFTPLGVLVALIFIGLPFVVRTVQ
                     PILEDLDTEYEEAAVSLGANRWQAFLHVVLPTVFPALLTGFAMAFARAVGEYGSVIFI
                     AGNIPLVSEITPLMIITKLEQYDYAGATAIAVVMLIFSFILLLIINGLQAWTAQRTGR
                     AR"
     gene            complement(26728..27702)
                     /locus_tag="PKX01_06510"
     CDS             complement(26728..27702)
                     /locus_tag="PKX01_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ75813.1"
                     /translation="MASSALLGVTAAHADTTLLNVSYDVSRELYKDINPAFAAQWQRQ
                     TGEKLTINQSHGGSSKQVQSVVAGLEADVVTMNQAPDIDVLARAGLVAGDWRKAYPHG
                     ATPYTTTTIFLVRKGNPKGIRDWSDLTRPGLQVIVPNPKTSGNGRYTYLAAWGYALQK
                     TGNEAGARDFVSRLFANVPVLDGGGRGATTTFTQRDVGDVLVTFENEATLIDQEVGGD
                     KFDVVYPSVSIEAAAPVAVVRKVVDKRGSRKAAEAYLNFLFSPEGQDIIARHHFRPRD
                     PQVLKHYARRFPSIRTFTVEDALGGWDAVQKVHFADGGVYDQVGVKHR"
     gene            complement(28129..>29697)
                     /locus_tag="PKX01_06515"
     CDS             complement(28129..>29697)
                     /locus_tag="PKX01_06515"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HMZ75814.1"
                     /translation="GGRIPLIIGRGLTSKAREALGLPVSTLFRLPQQPNDPGTGYSLA
                     QKMVGRACGLPEGKGILPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQ
                     SFCHTAAYPKLVDVKMHRELPTFIANRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDS
                     HTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGQMQPGITLRDLVNAIPLW
                     AIKAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELSDAAAERSAAACAIALNKE
                     PIVEYMRSNITLMKWMIAEGYEDPRTLKRRIAAMEDWIANGVLLKGDANAQYAAVIEI
                     DLAEVTEPILACPNDPDDVKVLSEVAGDKIDEVFIGSCMTNIGHFRAAGKVLDGKSDI
                     PARLWIAPPTKMDALILTEEGYYSVLGKAGARMEMPGCSLCMGNQAQIRKGSTAISTS
                     TRNFPNRLGIDTRVYLGSAELAAMCALAGRIVTKEEYMEQIKLVNAKAADVYRYMNFD
                     QIPAFVEVAETVEM"
BASE COUNT         4670 a         9571 c        10591 g         4865 t
ORIGIN      
        1 cacgcggcct caacattcga atcgcagcga tcaatgagct tccccgcgcc cgcatagtcc
       61 ttccccgcgg cgtcggagtc cttccacgat agccaaaacc cttaacacgc gggcccaaca
      121 ttcgaatcgc ggctatcaat gagcttcccc gcatccgcgg attccttccc caggcccgcg
      181 gggtccttct ctgcactcgc ggattccttc tctgcactcg cggattcctt ctctgcgacg
      241 tccaccaagc accccgcggc ctcaaagtcc ttccccgcac gcagggggaa gcactccgtt
      301 gcccgaaagt gtttccccgc cccgtcggtg aagcactcca ccgcgtgaac gaagccctca
      361 gccccgcgcg cttgcgctca gtccccgtcc agctccggca gcgccggggg caggtcgtcc
      421 aggcgggcgg cttcgatctt ctggaagtgg gcggtgatct tgcgggagga ggtctgcacg
      481 tcggccacgt cttcgccggc ctggcggatg tgggtggcga gtttctgcat gcgttcgtcg
      541 aagcgggcga agtctttggc gagcttgccg agggcgtcct ggatgacgtg gatctggcgg
      601 cgggtctcca cgtccttgag cacggcgcgg gcggtgttga ggacggccat gagggtggtg
      661 ggcgagacga tccacacccg tttggcgagg gcgtgggtga cgacttccgg gtggtaggcg
      721 tggatttcgg cgaagacggc ttcggcgggg acgaacatga cggcgccgtc ggaggtgacg
      781 ccgggcagga tgtacttgct ggcgatggcg tccacgtgct tcttcacgtc ggcgcggaag
      841 gcggcctggg cggtgcggcg gtcggcgtcg ccctggtcgg cggcgaacat gcggtggtag
      901 ttctccaggg ggaacttgga atccaccacc accaggccgg tgggcgcggg caggcgcagc
      961 acgcagtcgg cgcgggtgct gttgggcagg gtgtattgga agtcgaaggc gtccggcggc
     1021 agggcgttgc gcaccagggc ttcgagctgg acttcgccaa aggcgccgcg ggcgcgcttg
     1081 tcgccgagga gttcctgcag gctgacgacg ttggtggtga ggtcgccgat ctttttctgg
     1141 gcttcgtcga tggtggcgag gcgggccatg acgttgacga aggtctcgtt ggttttgcgg
     1201 aagccttcgt cgaggcgctg gttgacgtgg ccgccgatgg cgtcgaggcg ttcgcccacg
     1261 gtgcggccga ggccttcgat gccgtgggtg agctgggcgg aggcggcctg caggccggac
     1321 tggaggagct cccgatcggc gcgggcgtgt tcggagaggg tgcgcagggt ctcggcgagg
     1381 agttcgctgc gcaggcgttc ctgcccctga gcgacgccgg cgcccagctc ggcgaggcgc
     1441 agggcggtct cttcgcgctg ggtggcgagg cgggcctgga ggcgctcgcc ctgctccgcc
     1501 agcaggtcgg tgacgcggtc ggtagcggcg gaggtggcgg cctggagttc cttgacgaac
     1561 tggagctgct ggtgccccag caggtgttcg acacggtcgg cgaggctgtc ggcaaggtcc
     1621 tgggcgacgg cgtcctgacg acgggccagg gcggcgacct ggaaggccag ccagacgagg
     1681 aggacgaggc tcaggccgag ggcggcgagg agccagggga ggatttgggt ggaggcggtg
     1741 tcgggcatca tgggcgcgga gtatacccgc gccctgcccg gcttccgtcg gtggcgggga
     1801 cggtgtgtgg cggccggccg cggggatcag ccgagggcgc gcaggctggc caggggcggc
     1861 gtaaggagca ggcggcgggt gccgaaccag ccggcgaggg tcacggcgag gccgccggcc
     1921 agcgcgccga cgagcaccgg cagcaggctg ggggtgtagg gcagcgtgaa cacgaagcgc
     1981 ccgaggctcc agccgatgcc gctggcggcg accccggcga ggctgccgga gagcacgccc
     2041 agcacggcaa attcggccag cagggcggcg cgcagctggg cgttgcgggc gcccagggtg
     2101 cgcaggatgg cgagctcccg ctcgcgctcg tcgtgggtgg attgcagcgc accgaggagc
     2161 accaccacgc cggccaccac ggcaaagccg aagacgaact gcaccgcggc gacgagctga
     2221 tccaccatgc cctgcacctg ggccagcatg gcggccacgt cgatgaccgt gaagttgggg
     2281 aaccgggcga ccaggcgcgg cacgaagtcg gtctgcgccg gcggcaggta gaagctggtg
     2341 atgtagctcg ccggaaaatc cgccagcagg ccggggctgg cgatgacgaa gaagttcacc
     2401 cgcatggagt cccagtccag cttgcgcagg ctggcgatgg gggcttcgac ggtctcgccg
     2461 gccacgttga agcgcaggac gtcgcccatc ctgaggccca gagtctgggc caggccctgc
     2521 tccaccgaga agcgtggcgt ggtgtcgtgg ccgtgccagg tgccggcgat gacccgattg
     2581 cccggcggca gcgcggcgtc gtgggagagg ttgaagtccc ggtccaccag gcggcgggcg
     2641 cggtcgtcgg tgtagtcgtc ggggcccacc gggcggccat tcacggcgac caggcggccg
     2701 cggatcatgg gcagcagcac gggcgcgggc cggccggcgg cgcggaaggc gtcgcgcagg
     2761 gggtcgagct ggtccggctg gataccgatg atgaagcggt tgggggcgtc cggcggggtg
     2821 gcctgctgcc agctggtgag caggtcgccg cgcaccaggg tgaggagcag cagcgcggtg
     2881 aggcccatgc ccagggccag cacctgcacc aggctggcgc cgggtcgccg gcgcagggcg
     2941 gcgaggccgt agcgccagcc gctgcccgca ccggcgcgga tgcgtccgac gccgcgcagc
     3001 acgatccagc cgacgccggc gaagacaccg aaggcggcga cgaagccgcc ggtgacccag
     3061 gcgccgagcg tgacgtcccg cgccacgagc atcagcagcc cggtgagcac ggcggcgccg
     3121 aggctccagg cgaggccgtc gcgcaccgcc acgccgccca gctcgcggcg cagcacgcgc
     3181 agggtgggca cgcggccgag gcggatcagg ggcggcaggg cgaagcccag cagcagcacg
     3241 agggagacgg cgagcccgtg ggccaggggc cagacgctag gcggcggcag ctcgaagctc
     3301 accaggctgc cgaggaagcg ttccagggcg tattgcacgc caaagccgag ggcgctgccg
     3361 gcgaggccgc cggccaggcc cagcagggtg aattcgccga tgaagaggcc cagcagggtt
     3421 gtctggcggg cgcccaggca gcgcatcacg gcgcagccgt ccaggtggcg ttcgacgaag
     3481 cggcgggtgg cgaggcctac cgccaccgcg gcgaggacca ccgcgagcat ggcggcgaag
     3541 cgcaggaagc gttcggtgcg gtcgagggtg gcgcggatct cggggcgggc gttgtccacg
     3601 ctttcgaggg attcgccgcg ccccaggcgg gccttggccc aggcttcgta gcccgccacg
     3661 gcggcgatgc tgccggccag atggaggcgg taggtgacgc ggctgccggg cacgatgagg
     3721 ccggtggcag gcaggtcggc ggcgttcatg acgatgcgcg gcagcagggc aaagaagttg
     3781 gcgccccggt cggcctcgaa gctgagcacg gcggccatgc gcaggcgcga gcggccgagg
     3841 ccgacgagat cgccagggtg ggcgccgagg gccgtcgcgc tgcgttcgtc gagccacacc
     3901 tcgccgggtg cggggatgcc gcgggcgggg gcgtccggct ggttgaggcc gggggcgatg
     3961 cggatctcgc cgcgcagggg gtggcccgcc tccaccgact tgacgccgac gagctgggtc
     4021 gcgtcgccga ggctggccat gctggtgaag gtggtggatt cgacgctggc gaggccgcgg
     4081 cgggcggctt cggcgcggaa gtccggcgcc agcgggtggt cggcggcgat gagcaggtcg
     4141 ccgcccagga gctggctggc ctggcggttg aggccgcggc cgacccggtc ggtgaagaag
     4201 ccgacgctgg tgaggctggc gacggcgatg acaagggcga cgaggagcag accgagctcg
     4261 ccggcacgca ggtcgcggcg gagcatgcgg aaggacaggg cgaggcgctg gcggacggtc
     4321 atggagcgtg aggccgcggc ggcgcgggaa gattcccggc tgcggcggaa attttgcggt
     4381 gcgtcacgct agcacaggat gattgccctt tcagccgccc tgccctgcct gcctgaaacg
     4441 cccggccggg gcggaccgtt gcgcccggtg tgtgttccgg ctgccgaggg gcaccggccg
     4501 ggggacacag acaaacggca cccgaaggtg ccgttgcccg cgtctgcaga aggcctcagt
     4561 tcttccgccg acgcattccc aggagcgcga tgagtgaagt gccgcccagg agcgccaggc
     4621 tcgagggttc cggcaccttg gtgtaggtgc cggtgacggc tgcgatcttg atgtaatcgt
     4681 tgccgctgcc cagggtggcg ccgttgggca gggcctggac cagggtgttg agggcgccga
     4741 tcagccagta gcgggcgctg acgttgagat cattgaccgc cagggggatg taacccgccg
     4801 agttgctgga ctgggacggc gcgccgccgg ccgcatccgc gtagttggcg gtgccggtgg
     4861 tgccggcgct ggatttgacg gtggtccacc cgctgctcag cagggaggcg tagccctggt
     4921 tggcggaggt gaatgtgggg gtgccggtgc cagtgtaggc cagcacggtc aggtcggagt
     4981 cggagtacca ccagccgacg gtgacgctct ggagcttcac cgcgttgccc gcgccgaagt
     5041 cgaagagcat cgagtcgtag cgggcgttgt tgtccatcgt gtggccaggc gacgtggtgt
     5101 tggcgatggt gccttcgttg gtgtcgccgg caccggtggc gccgtcccgg ttggtcacgc
     5161 ccagaccgcc ggagtaggtg cccaggtaag cgctctcgat gtaggcgttg cccgcagtgg
     5221 tgctgttctt ggtgtcggac caggccgaga tggtcaccgt ggagccggtg ctgccgtagg
     5281 tccgggtgtt gccgaagtta ccggcgccgg tttcggtccc cccgctggtg agggtccagg
     5341 cggtcgcggc gtgggcgccg gcgacgctgc cgatcagcag ggcgaaggtc aatgccgttt
     5401 ggctgggaat gcgtttcatg tcgggaggtc ctattttcgt ttcgacacgg ctgtcgttgt
     5461 tgtcagatat gtagcaatta ttctgccaat tcgctattta cttgtttttg tttaaaaatc
     5521 gcagtgcacc acgtttatgg caaggcaaac tgtaaaatct atcgacaacg ttaaaaattg
     5581 tttaaacacc cagctcgcgg cgcagatttt gtatttcaat gtcgtagccg cttccattcg
     5641 tactgttcag cgcgccttgc agttcagcgc gtgcttcgtc ccgcaggccc ttgcgaagga
     5701 ggacccaggc caggtggtag cggatctcgc gggactcggg ggcgcgcagg cgggcttcgc
     5761 gcagggcttt cagaccgatg tccagcgccc cgctacgggc ccgcgcccag ccgacggtgt
     5821 cgagcacgtt ggcgtcgccg ggcgcaaggc ggtaggcggc ttcggcggcg ttgagggcgc
     5881 ccgggtcgtt gagggccatc aggatctgcg ccaggttgtt ggcgacgttg gcgttcgccg
     5941 gctcggctac cagcacgcct tcgtagaggc ggcgtgcgtc ggccagatgg cccagacgga
     6001 aatgggattc ggccagggcg ctgcgggcgg tcaggtcgtt cggatgggtg cgcagccagg
     6061 tgtccagcag ggacacggcg cgatcgccct ccccggcctg gaacgaggcc tcgacacccc
     6121 gcaggacgag cgccagggac ggggccttgt cgaaggcgct gcggtagaga cgggccgcat
     6181 cgcggggctg ttggcgggcg cgggcaaggt cgccggccag gcgcagggcc tcggcgctgt
     6241 tgccgctgcg ggtcgcgaag gatttgagga gggcgtcggc gcggttgaga tcgccgaggg
     6301 ccatctcgct ttcgatctgc aggatctggg ctggcacgag gccggcgttg cgcgccaggg
     6361 ccttctcggc gctggccgac gcttcgcgga aggcgccggc atccagttgc aggcgtccga
     6421 cggccaccag ctgtccgggt tcaagaccgg gcaggcggga cagggtattg aaggtgctgc
     6481 gcgcatcggc cagattattg gcggccaggt gcgcgcggcc gagggtttcg aggacgagcg
     6541 gatcgtccgg acggctgagc gccagctgct tggccaccgc gacggccggc gcaggcttgc
     6601 cgagcttctg gtagagggcc agaagctcct gggcgacggt cgggtcggcg ggcttgaggg
     6661 cataggcctt ttcgagcagg gccaacgcgt cggcgctacg gccggcgcca agctccaggc
     6721 gggcgttttc ggtgagcacc tgcacatcgg ccgggtgtgc cttgagcagc gcggcaaggc
     6781 gctggcgggc cgcgtcggcg cgtccttcca cgagatccag gcgggccagg ttcaactggg
     6841 ccggaaggaa gccgggcgcc agcgtgatgg cctgggtgta ttcggcgcgg gcctcggccg
     6901 gcttgcgggc cgcgcccttc acggcgccca gcaggttgtg ggcggcggga tcggacggca
     6961 ggcgccgggt ggtggcttcc gccaccgcca gggcgttgcg gaggtctccg cggcgcaggt
     7021 acagggtgga cagggcggtc gccacgttga gttggcccgg ctccttgtcg aacgcgctgc
     7081 gcagggcgct gaggccgatg tcgggctgca tgttggcgat gcgggcgaag cccatctggg
     7141 cggtgatgcc cggattgccg gtgaggcgcg cggcggcttc gagggcgtcg gcggcgtccc
     7201 ggtagcggcg ctgctgcatg cgcagggcgg ccagggtggt aagggcctgg ggatcggcgt
     7261 cgccggaact gatgaggggc tggagcaggt cgccgacgcg ggcggtctcg ccggcggcga
     7321 ggtagatgtt ggcgagcatc ttgcggccgg cggcgtcgcc gggcatgcgc cggacgtaga
     7381 gttcgaggag cgcacgggcg cgctcaccgc ggcccagggc ctggctggca aggccgccga
     7441 tcatcagcag ggtgggctgg cggccgacga attcctgggg cagctcgtcc accaggcgga
     7501 ccacgtcctc cagctgggcg cgggaggcct gggtgtcgcc gcgctgggca tagaccaggc
     7561 tcttgaggta ggcgacgcgg ggctcgcggg gtaccagggc ggcggcgcgc tccagatccg
     7621 gcagggcctc gttggcgcgc ttcaggtcga tgagcaggga cgcgcgtccg acgagagcgt
     7681 cgagcatgcg ggggtcggcg gcgatggcgc gattgaaggc ctccatggct ccgcgggcgt
     7741 cgccggcgcc ctgcagcagg ccggcctgca ggtgcagcag gcgggcgtcg ccgggggcga
     7801 tgcccagggc ggtgtcgagg acctggcggg cgcgggcgct gtcgcggctg cgcagggcca
     7861 ggtcgatttc ggcgagcaag ggcgacaggg cgcggggatc ggcctcacgc gccagcttga
     7921 cctcggcgac agcggcacgc atgtcgcccc ggtcggcctg ggcggtggcg cgcatggcga
     7981 gcacttcggc gcgcaggcgc aggggcaggc cgtcggcctt gatgcgctcc agcagctttt
     8041 cgctgttgcc gaacatcatc agggcctggc cgtattcgag ggcgatctcg gacggattca
     8101 cgcccaggcg cagggccgct tcgaactcgg cctcggcggc ggcggcctgg ccgtccttga
     8161 gcagggcgcg ggccagcagc aggtgggcag gcagctgttc ggggtcgcgc tggagggcgt
     8221 tcttggcctg gatgatggca ccggcggcgt cattgcgggc tagacgggcg gcggcatcct
     8281 cgtagtagcg ggtggcgctg tcggaggcgc ccgcggccca gccgggcagc gtcaggcaca
     8341 ggcacaggag cgcggcgggc aggcggcgga gggtgacatg aggctgttgg gaagtcggca
     8401 ttttcagacg gcatcgtgcg atgacaggcg ataccgtctt tgtaatcgcc ggccgggaca
     8461 cgggccgtga caaatgccgc cacagagcct gtggcgcccc gcaattccgt cacgctgaag
     8521 agtcgatcac cagaaagtga agacaaatgg cacggtttct ccgcggggag acgtgtgcgg
     8581 aggggatgtg ccggaggtcc gccgggcccg aagaatgcgc cggaaaccgt accggtcggc
     8641 gccccgtcgg gcggggcgtg gtgttcaggc gtcggcgagc cagcgttgca cgagacgcac
     8701 ccaataggcg acgccatagg ggatggcggc gtcgttgaag tcatagtggg gattgtgcag
     8761 ggtgcagccg ccctcccccg gcccattgcc gagccagatg tagcagccgg gcttttcctg
     8821 aaggaaatag gagaagtcct cggcgcccat gctcggccgg ggatcgacgc tgacccgggc
     8881 atcgccgcac acctcggcgg ccacgcgggc acagaattcg gcctcggcca cgctgttgat
     8941 ggtcggcgga tagccgcgtc ggtactcgaa atggacgttc gccccgaagg ccgcaccgac
     9001 gccggcgcag atgcgttgca tggcgtcctc gacgcgggcc tgcagttcgg gtctgaatgc
     9061 gcggacggtg ccgccgatgc gggccacatc ggggatcacg ttgtaggcgt cgccggcctg
     9121 gaactgggtg acggacagca ccaccgcgtc ctggggatcc gtgccgcggc tgaccaccga
     9181 ctggagggcc tggaccagag cggctccggc gagcaccggg tcgatgccct gatggggcat
     9241 ggcggcgtgg gcgccgtggc cgcggatctc gatgtcgaag cggtcggcac aggccatcac
     9301 tgcaccgcga tgcacggcga tttcgcccag aggcaggccc ggccagttgt gcaggccgaa
     9361 cacggcgtcc atcgggaagc gctcgaagag gccgtcggcg atcatttcgg gcgccccgcc
     9421 ctccccttcc tcggccggct ggaacaggaa atacacggta ccgtcgaatt ccgggtgacg
     9481 gctcaggtgg gccgcggcgc ccaggagcat ggcggtgtgg ccgtcgtgtc cgcaggcgtg
     9541 catgcggccc gggtggcggg agtggtgggc gaagtcgttc ttttcctgaa tgggcagggc
     9601 gtccatgtcg gcccgcaggc caatggcgcg gcgcccggag ccggccctga gcacgcccac
     9661 gacgccggtc ttgccgatgc cggtgtgggt ttcgatgccg agggcgcgca ggcgttccgc
     9721 caccaggccg gccgtgcggt gctcctcgaa ggcaagctcg gggtgcgcgt ggatgtctcg
     9781 gcggagcgcg gtgatgtcgg cgtgatccgc ccgcagggaa tcaaggaggt gcatggtgca
     9841 ttcgcgtgtc cggcccgcgg gccgtatggt tcaatgatag cagccgcctg ccgccctgct
     9901 cccgatgaag ctcttctacg ccgaccattt cgtcctgccc ctgccgccgg ggcaccgctt
     9961 ccccatggag aagtacgccc gcctgcgcga gcgcctgcag gcttcgggcc tgttccgtcc
    10021 ggacgatttc cacgaaccgc cacctgccac cgacacggag atcctgcgtg cccatgacgc
    10081 gggctacctg cagcgggtgg tccgtgggca gctgacgccg gacgagctgc gcctgatcgg
    10141 ctttccgtgg tcgccggcca tggtggagcg ttcgcgccga tcgtccgggg ccacgctggc
    10201 ggcctgccgg gcggcgctgg aggacggctg cgcggtcaat ctggcgggcg gcacccacca
    10261 cgccttccgc gaccgggggg aaggcttctg cgtcttcaac gacgcggcca tcgcggcgct
    10321 ggccatgcgc gccgagcacc gtgtggagcg gctcgccatc gtggattgcg atgtgcacca
    10381 gggcaacggg accgccgcca tcctggcgca tcgggccgac tgcttcacct gcagcctcca
    10441 tggcgcgaaa aactatcctt tcgacaagga acgcagcgac ctggacgtgg aactgcccga
    10501 cggtaccgcc gacggtcctt atctggacgc cctggcgatg gcgctggaga ccgtgttcgc
    10561 gcgcgccgat ccccagctcg tgatctacct ggccggcgcc gacccctacg ccgatgaccg
    10621 ccttggccgc cttgccctga gcctggacgg gctggccgcg cgggaccgtc tggtgctggg
    10681 cgaatgccgg cgccgcggcg tgtcggtggc gatcgccatg gccggcggct atgctcgaca
    10741 gatcgacgac accgtggcga tccacgcccg caccgtcacc ctcgcccggc agcgcttcgc
    10801 gccgggcccc acggaggccc catgagccaa gaatcccaca tcgccctgct catcgacgcg
    10861 gacaactgcc ctgccgccaa gatcgaggag atcctcgacg aactggccaa atacggcgtg
    10921 atcaacatcc gccgagccta cggcaactgg aagagcccgg ggctgaagag ctgggaggcg
    10981 gtgctccacg aatacgccat ccagccgatg cagcagttcg cctacaccaa gggcaagaac
    11041 gccacggatt cggcgatgat catcgacgcg atggatctgc tctacagcca gaacctggac
    11101 ggcttctgcc tggcctcgtc ggactccgac ttcacgccgc tggtgatgcg catccgtgcc
    11161 aacggcctga aggtcttcgg tttcggcgag aagaagacgc cctcgccttt cgtgaatgcg
    11221 tgctccaagt tcctgtacct ggaaaacctc ggggcgaccg acgccgatac gccggcatcc
    11281 gaggcgccgc cggcggcggt tccgtccgcc cgggccgaat ccgatggcgg agcgggacgc
    11341 ggtactgccg ccggtgacgc gggcaaggcc gccgagtcca gcacgcgccc ggtcgcccgc
    11401 cccactccgc gggagctgcg cggcgacgca cgtctggtca atctgctgcg caagtcggta
    11461 gaggccgccg ccgacgacga cggctgggcg gcgctcgggg aagtcggcca gcacatctcc
    11521 aagcaggcct ctttcgattc gcgcaacgtg ggctacgccc gcctgcggga tctgatgcag
    11581 gacatcggcc tgttcgacgt cagccagcgg gacaaggcca tctacgtgcg cgacaagcgc
    11641 ctggcgcgca agcccgcgcc ctgaaccgcc cgcgccggcc accccgcgcg ccgctggcta
    11701 taatcgccct tcccccaaga ggactgcgcc catgcgattc gaaggtaccg agacttacgt
    11761 tgccaccccc gacctgatgc tggccgtcaa cgcggctgtc accctgcagc ggcccttgct
    11821 catcaagggc gaaccgggca ccggcaagac cctgctggcc gaagaggtgg ccgccgcgct
    11881 gggcatgccc ctggtgcagt ggcacatcaa atccaccacc aaggcccagc agggcttgta
    11941 cgaatatgac gcagtgtcac gcctgcggga ttcccagctc ggtgacgacc gggtcaagga
    12001 catcggccag tacatcgtgc gcgggcccct gtggcaggcc tttgaatccg acgtcccctg
    12061 cgtggtgctg atcgacgaga tcgacaaggc cgacatcgaa ttccccaacg acctgctgcg
    12121 ggaactggac cgcatggagt tctacgtcta tgaaacccgc cagttggtca aggcgcgtca
    12181 tcggcccatc gtgttcatca cgtccaacaa cgagaaggag ctgccggacg ccttcctgcg
    12241 ccgctgcttc ttccattaca tcaagttccc ggaccgggac acgatgcaga agatcgtgga
    12301 tgtgcacttc ccgggcctca agccggccct catccaggag gccctgcagg tcttcttcgg
    12361 cctgcgcgag gtgccgggcc tcaagaaaaa gccgtccacg tccgagctga tcgactggct
    12421 gaagctgctg gtggcggagg acattccgcc cgaagccctg cattcgacgg accgcaaggt
    12481 catcgtcccg cccctgcacg gggcgctgct caagaacgag caggacatgc acctcttcga
    12541 gcgcctggtg ttcatggccc agcgcaaccg ctgagcgcgg cgcgatgctg atcgatttct
    12601 tcctgcatct gcgcgaacgc aagctgccgg tctccaccaa ggaattcctg accctcctgg
    12661 aagccctgca ggcgggtctg gcgcggcaca gcctggacga cttctacttc ctggcgcgca
    12721 gttgtctggt gaaggacgaa gcccatttcg accgcttcga ccaggcgttc ggcgaatact
    12781 tcaagggcgt gcaggcgatt cccggcctgg acgtggatct gcccgacgag tggctgaagg
    12841 ccacgatgaa gcgccacctc agcccggcgg acaaggccaa gctggaaaag ctgggctggg
    12901 acacgctgat ggcggaattc aagaagcgtc tgcaggaaca gcgggagcgc cacgcgggcg
    12961 gctcgaaatg ggtcggtacg gggggcactt cgcccttcgg caacagcggc tatcacccgg
    13021 aaggcatccg cgtggggggc gaatcggccg gcaatcgcac cgcggtgaag gtgtgggacc
    13081 ggcgggagta ccgcaacctg gacgattccg tcgaactggg cacgcgcaac atcaaggtcg
    13141 cgctgcgccg gctgcgccgc ttcgcccgcc tcggcgcgcc cgacgagctg gacctggacg
    13201 gcaccatcgc cgccaccgcg cgcaatgcgg gctacctgga cctgctcatg cggccggagc
    13261 gccacaatgc ggtgaaggtg ctgatcttct tcgacgtggg cggctccatg gacccccacg
    13321 tgaaggtctg tgaggaactg ttttcagcct gccgggcaga attcaagcac ctggagtact
    13381 tctacttcca caactgcgtg tacgaccacg tgtggcgcga cagcacccgg cgctataccg
    13441 agcgcacgcc actggcggac gtgatccaca aatatggccc ggattacaaa ctgatcttcg
    13501 tcggtgacgc gaccatgagt ccctacgaga tcctgcagcc caacggttcc atcgaatacg
    13561 ccagcagcga ggccggcgcc gtctgcctgc gccgtctggc ggacgcctat ccggccctgg
    13621 cttggctcaa tccggagccg gagcgcacct gggagtttcg ccagtccatt ggaatcattc
    13681 gcgaactgct cggcgagcgg atgttcccct tgaccctcga cggcctgacc cgcgccttcg
    13741 cgcacctgtc gcgcaagcac tgagcccgga aaaggcatcg gccgcggacg atggccgcgg
    13801 ccgacatgca gaccacccgc agctgcgact tacggcgccc ggtagttgtc ctgggtcatc
    13861 aggtcgatgc cgcaggggag gttctcgatc cagtcgagga agcggttcac catcgccttg
    13921 ccctcgaagc ggcagccgtg ctggggcacg atcatgtcca cgtccagctg gcgcaccatg
    13981 tgcacccagt agcggcagat cttgtttgaa atcatgtaac ggcgatggaa gccggccatg
    14041 gtgatgacgt gatcgtcgaa gttgcgcact ggctcggcag cctgggcgtg gtctacaagg
    14101 gaggtgccca tgtcgccgga aaagaggatc ttggcgaccg gatcgtagaa ctggaaattc
    14161 ccctcggaat gcatgaagtg ggcgggtagc gccttgatgc ggctgtcccc cagcgcgatg
    14221 ttcatgccct cgtcggggat gccggtgata cggctggtgt agtccttgcc agtggtgaaa
    14281 tggggcacga agcgcgccca caggcgggaa atcatcaccc ggcaatgggt tgtcaccatc
    14341 cacttgttga gcgacgcgat gatgtccgga tcggcgtggg tggcaaagat gtaatccagc
    14401 ccgcgggatg ggaagaagcg ctgcatggcc atcagcaggc cgttgtaggt catgttgccg
    14461 ccaggatcga ccagtgcgcc gtgttcgccg tcgacgatca ggaactggtt gcactggacc
    14521 gcgtgatcgg cctcttcgtc caccaggtcg taaaaggcca ggcaggcgtg cttgccatca
    14581 ttgaagagtt ctaccgccat gggtgcgctc gtgtgtcaaa aagtcattat ttgcaattgc
    14641 gtgacactgt aacgggttcc ctgaaagcct gaaagccctt ctctagattg cgatgaggcc
    14701 gatgccctgt attgcagtgc aagaatggag tatggttcgc actcccgatt tcaaccgctg
    14761 gaagattgtt cccgatgcgc cgcttcgtcc gctctttcgc cgtcacctgc ctcctcccct
    14821 tgagcgtgtt tgggatgccc gctgccgctc agagtctgtc ctcctcgtct gcaggaaagg
    14881 ccagaatggg catgtcgtcc gggttcggct catccgggat gggctcggcc aacacactct
    14941 ccggcggaat cggaacccgc agcctactgg attccaaaca gaacggcccg cagatcaagg
    15001 atttgcggcg cccgtccgcg tccgaatgtg cgatgcgtga actggcgggc aagaagtcgc
    15061 cgggcatgac ggccaatggg caagcgggca gcggcaaagt gacgacgtac acggatgcat
    15121 cgtccctgcg cggacgtagt ggcgacatct acggtgattg cgacggagcc cagggaatgc
    15181 gtcgcagggg cgcgggagga agcaccggaa cgtcgtcgtt cgtcgggatc ggtagcgcta
    15241 cacgtcccca gaccgcctac gaccggcgta ccggtacctt gaaggctgcc aaggacggct
    15301 atgtgaaccg tggggcgacc agttccatga tgcccaagca tccggagtcc gcttatgacc
    15361 cggtgaccgg cacgctcaaa ccgccccggg tgcaaagcgc gtaccagatc tatcagcagc
    15421 aataaaacac agggcgatgc agaacggccc tggaggccct gcatcgccct gggttgcgga
    15481 gcagcgcggc cggagggcgg ttgaaccctt ccggcacgtt cgacctcagc ccgcgttcat
    15541 cgccagcatc agctggttga tccgcttcac gaagctggcc ggatcatcca gggtgccgcc
    15601 ttcggcgagc agcgcctgat cgaagagcac cgccgcccag tcgtcaaact ggccggtctc
    15661 gtacttgaga cgcagcacca ccgggtgggc cggattgatc tcgaggatgg gcctggacat
    15721 gggcatgctc tgaccagccg ccttcatcag gcgggccaga ttcatcccca tgtcgtgctc
    15781 gtcggcgacc aggcaggccg gcgaatcggt caggcggtgg gtgatgcgta cgtccttgac
    15841 cttgtcaccg aggctgccct tcatcttgtc gaggagttcc ttgaattctc cggtctcctt
    15901 ctcgacggcg gccttctcgg cttcgtcctc cagcgtgccc agatccaggc cacccttggc
    15961 gaccgaggcc agcgccttgc cgtcgaactc ggtgaggtgg ccgacgaccc attcgtccac
    16021 gcggttggac aggagcagca cctcgatgcc cttcttgcgg aagatttcca ggtgcgggct
    16081 gttctttgca gcgttgaagc tgtcggccgt gacgtaatag atcttgtcct gcccttcctt
    16141 catgcggccg atgtaatccg ccagggagac ggtctcgtca gccgtgtcgg cctgggtgga
    16201 ggcgaagcgc agcagaccgg cgatcttctc cttgttggag aaatcctccc cgacgccttc
    16261 cttcagcacc tggccgaatt ccttccagaa ggtggtgtac ttgtccttct ggttctcggc
    16321 caggtcttcc agcagaccca gcaccttctt cgtgcagccg gcgcgcaggg tgtcgatgtc
    16381 cttggattcc tgcaggatct cccgcgacac gttgaggggc agatcgttgg aatccaccac
    16441 cccgcgcacg aagcgcaggt agaggggcat cagcttctcg gcgtcgtcca tgatgaagac
    16501 gcggcgcaca tagagcttga cgccgtgacg ggcctggcgg tcccacatgt cgaagggggc
    16561 atggccgggg acgtagagca gctgggtgta ctcgtggcga ccttccacgc gagagtgggt
    16621 ccatgccagc gggggctcgt agtcgtggcc cacgtgcttg tagaactcgg tgtattcctc
    16681 gtcgctgacg tcggacttgg gccgcgccca cagggcgttg gccttgttga cggtctcgtc
    16741 ttcgtcggtg acgacctgtt ccttcttgtc ctcgtcccag ctttccttct tcatcacaat
    16801 gggctgaaga atgtggtcgg agtacttgcg gatgatggac tggagcttcc aggcggacag
    16861 cagatcgtcc tggtcctcgc gcaggtggag ggtgatctcg gtgccgcggg atgccttttc
    16921 gatgggctcg acggtgtatt ggccggccgt gtcaccggtc atggtgcact cccatttcac
    16981 cgcgtcaccg gccgacagcc cggcccggcg ggtgcgcacc gtcacggtgt cggcaacgat
    17041 gaaggccgag tagaagccga ccccgaactg gccgatcagg tgcgcgtcct tctgctggtc
    17101 gccggtcagc ttgccgaaga actccttggt accggatttg gcgatggtgc ccaggtgggt
    17161 gaccacttcg tcacggctca tgccgatgcc gttgtcggcg atggtcaggg tccgggcggc
    17221 cttgtcgtag gcgacgcgga tcttcagttc ggcatcctcg gccagcaggg cgccgttctc
    17281 aagggcctcg aagcgcagct tgtcgcatgc gtccgacgcg ttggagacca gctcgcgcag
    17341 gaaaatttcc cggttggaat acagggaatg aatcatgagg tgcagcagct gcttcacctc
    17401 ggcctggaag ttcatcgttt gggcggacat ctgttcgaca gccataaagc ttgctccata
    17461 gggaccgtaa acgtcccaca tatgggtgcg tccacgcatc tttcaagtcc cgtccgcatc
    17521 tttcatggaa ccttcccacg cttcaccgtc cggcttcgcc gaatccgacc gcccggcgga
    17581 tgtcctgcct gccggaacgt cgaagggccg acgtttcagc cgacagcgga tcgcgatctg
    17641 gggcggtttc gcgctgcttg ccgcgctcgt ggcggccgtc gcggtgtccc tgctgcgcta
    17701 cgaccgggcc caggccctgc atcgcgccga ggcggaactg cggggcctga cccgcgtgct
    17761 cgaagaacac gtcgcccgca ccttcggcga gaccgacagt gcactcagcg accttggcca
    17821 gcagctgagt ggccgtctgg ggagcgacgc gccgtcggag cgggacctca acgctctcct
    17881 ccgccagcgc gcagcccatc tcccgcaggt cgaatacctg tatgtggagc gggaggacgg
    17941 cagcctcctc gcggacacgt tcgacctgcc gcgcggcgcg gaagccgaag cgccgaagcg
    18001 cggtctgccc accctgcacg acggcctcga gatcgcggcg cccgtccgca gtgcccattc
    18061 gggccactgg gtcacccccg tcaaacgcca gatctacgac gcggccggca agttcgtcgg
    18121 ggtcgttggc gccgccatga gccaggacta cttcaacggc gtctaccgtg acctgcagat
    18181 ctttccggac gatgccatcc tgatcgtcga tgccacccgc ggcacgctcc aactgcgcca
    18241 ccccccgacc gatgggcagg tcggtgcgcc cctggggccg tcctctgccc tgggactgga
    18301 tgcaaaccgg cgcagcgcgg tgagcatcga gggcgacgag acgcccgccg cccgcctcac
    18361 cgccttccgg cgtctggcag actacccgct ggtggtgggc gccagtcgcc cgctggagcg
    18421 cgtactggcc ggcttcagcg acaactatcg gcgcatcagc gccggtgcgg ccctgctgat
    18481 cgcgctcctc ggagtgctcg cgctgctcct gcaccgggac agcctgcggc gcgacgggga
    18541 ccgccaatcc ctggccgagg tcaatgcgaa tctggaagag cgcgtccgta cccgcacggc
    18601 cgagctggag cagtccaatc gggaactggt cagtttcagc tattccattt cccacgacct
    18661 gcgcgctccg ctacgggcca tcaacggcta cgcccatctg ctcgacgagg aatacggcga
    18721 ccgcctcgat gaaggcggcc acgcttacct ggcccgtctg cgcaaggcca gcctgcgcat
    18781 gggcgaactg atcgacgagc tgctgaaact cgccaatgtc tcgcgccagc ccctgcacat
    18841 cgaattgacc gacgtcagca ccatggcacg ggagatcctc gacgaatttg ccgcccagtc
    18901 atccgggcgc gtcgtccaca tccacgtgga agacggtctc gccaccgagg cagaccccaa
    18961 tctgctgcgc acggccctgc agaatctcct gggcaatgcc tggaaattca cccgcgacag
    19021 ccagcccgcc ctgatccagg tgggcggccg accccatggc caggagcggc tgttctatgt
    19081 caccgacaac ggcgtcggat tcgacctggc ccacgctggc aagctgttcg agcccttcca
    19141 gcaactccac aggaacgctg gttttgaagg ctccggcatc gggctggcca gcgtgcggcg
    19201 catcgtcgaa cggcatggcg gctcggtgtg ggccgagtcg gcgcctggcg ccggcaccac
    19261 gatcttcttc accctgccac gcagcccggc catcgttcgc cgcccgcggg agcgggtgca
    19321 ggtctgaacg gcgcgccgct cattcgtagc ggatctcgac gacctcgatc tcgcgcacgc
    19381 cggccgggct ggcaaagcgc accacatccc cctcccgcgc cttcagcaag gcccgcgaca
    19441 gaggcgcgat ccagctgatg cggccttctg aagcattggc ctcgtccacg ccgacaatct
    19501 ggtaggtctg ctcggcgtcg gcgctggcgt cgaacaccgt cacggtggcg ccgaagaaga
    19561 cctgctcggt gttctcctgc tcggacggcg tcaccacggt tgcggtttcc aggcgcttga
    19621 ccaggaagcg tacgcgccgg tcgatctccc gcaggcgctt cttgccgtag atgtagtcgc
    19681 cgttctccga gcgatcgccg ttggaggcag cccagcgcac cgtctccacc agggccggcc
    19741 gctccacccg gacgagctgg tcgagttccg cccgcagacg ctcgaaaccg ttgcgggtca
    19801 tgtagttgcg ggtgcccggc ggcaggctcg gtgcgccggc cggcgcatcc tcttcgtcgt
    19861 cgtccgattc cttgacgaat gccttgttca cctggagtcc cttcagtgcg cgcattgcga
    19921 ggggggcaat ggtaggagcg ccgcgaccgc gacgcaagcg gcccgatcac gctcacgggg
    19981 cgctatgatg ggggcccgcc cgtcaccgga gtctgccgcc atgcatccga tcctgtccgg
    20041 caaacgcgtc ctgctcaccc aggccgactg cttcatgggc cccgccctgc agcgtgtctt
    20101 cgaggcggct ggtgccgccg tgatcgctga cgaccgcccc ctgctcgacc cggcgctgcc
    20161 ggaagcgctg gtccgtcagg ccggcgacat cgacatcctg ctggcccatc tcgcggtgcc
    20221 gagcccgcgt acgccggcca ccgacgtcgc cgatgacgaa tggcgcgagg tcttcgccca
    20281 tctggtggat cccctccccc gcctggcccg cgccgtgctg cccggcatgg tggcccgcgg
    20341 cagcggtcgc attctcgtcc tgggcagcgc gtcggccctg cggggcatgc gcaatgcctc
    20401 gacctacagc gctgcccgtg gcgcccaact ggcctgggtg caggccctcg gcgtcgaggt
    20461 ggcgcggagc ggcgtgcggg tcaacgccat cgcccagaac ttcgtcgaga accccaccta
    20521 ttttccccca gaagtccagg ccaacccggc gttccaggag cgtctggcgc gggaggtgcc
    20581 cctcggccga ctggtcagcg cggacgagga cgcccgcttt gcggcctatc tgtgctcgga
    20641 tgacgccgac tgtttcgtcg gtcaggtctt tcccgtgtgt ggcggctggg ccgtccgctg
    20701 aacccgctcg aacaggtcaa tgcccatgcg cctgcaggaa ctgttgtcca ttgaatggcc
    20761 cctcatccag gcgcccatgg cgggcgtcca gggcagcgcc ctcgccatgg ccgtcagcca
    20821 ggccggcgcg ctcggctccc tgccctgcgc gatgctgacg cccgatgcgc tgcaggccga
    20881 actggccgtc ctcagcgcat cgaccgcgcg agcctacaac gtcaatttct tcgcccacac
    20941 gccgccgcag ccggatgggg cgcgggaggc ggcctggcgg acgcagcttt caccttatta
    21001 ccggacattc ggcatcgatc ccgcgggcat tgctacgggg cccggccgga tgcccttcag
    21061 cgcggccatc gccgatctcg tcgaaccctt ccgcccgccc gtggtgagct tccatttcgg
    21121 tctgcccgat gccgccctgc tggcccgcgt caaggcctgg ggcgcccggg tgctgtcgtc
    21181 cgcgacgacc gtcgaggagg cgcgctggct ggccgcgcgg ggcgtcgacg ggatcatcgc
    21241 ccaggggctc gaggccggag ggcatcgggg ccatttcctc gaccacgatc tcacggtcca
    21301 gtctggcacc ttcgcgctgc tgccccagat cgtccgcgcc gtgcaggtgc cggtcatcgc
    21361 ggcaggcggc atcgccgatg cggacggggt ggctgccgcc atggccctcg gcgcggcggg
    21421 tgtccaggtc ggcaccgcct acctcctgtg tccggaggcc accacttcgc cgattcaccg
    21481 ggcggtcctg cgcacggagt ccgcccggca cacggcgctg accaatctct tctccgggcg
    21541 cccggcccgc ggcatcgtca atcgcttgat gcgcgaactg ggcccgctca atccggaagc
    21601 gcccgaattc ccgctggcga cagcggcggt tgcgccgctt cgcgcggcgg ccgaggcgct
    21661 gggttccggc gacttctcgc cgctctggtg cggggagaat gcgtctggct gtcgggaaat
    21721 ccctgccgct gaactgaccc gcgccctgat tcagggcttc caacggcggg agaccgcgtg
    21781 ctgacgggcc acgcggaggc gcccgtgccg tgtcgtgaag cgtgcggcgc ctgctgcatc
    21841 gccccgtcca tttccagccc gattcccggc atgcctgagg gcaagcccgc gggcgtgcgc
    21901 tgcgtccagc tcgacgcggc ggatcgctgc cgcatcttcg gacaagcgga gcgtcctgcc
    21961 gtgtgcggct ccctgcggcc gacgcagtcc ctgtgccgcg gcagccgcga ggaggcgctg
    22021 caggccctcg cctggctcga ggcgcacacg gcgccgggct gagtccattc cgcgccggtg
    22081 cgtcccgggc gtccttcgtc cattcgccgt catccgtatt gcggcaggaa gaataagcat
    22141 tccggatcat attggttcgt cggccggact gaacgtccta gactccggcc catctccttc
    22201 gccggacgcg ctgtcgtgcc ccctccccag acccctgctg cgaccctcat tgtccccggc
    22261 tacctgggca gcggcccggc ccattggcaa agctggatcg aatcccggat ccccgacacc
    22321 cgccgtgtcc gccacgtcga ttgggacgcc ccggtgctgg cgcgctgggc ccaggcggtg
    22381 cgcgacgaga tcgacgccgc cgccggtccc ctgtggctgg tggcccacag cttcggctgc
    22441 ctcgccagcg tggtggcggc ggctgatcgc ccggacaagg tggcgggcct gatgctcgtc
    22501 gcgcccgccg acccggagcg cttcacgccc cgcggggtcc gtacgtccac cggcggcgtg
    22561 acggagcccg gtctctccgg ctgtctgccg cgccgcctgc tgcccatgcc cggtctggtc
    22621 gtggccagca ccaacgatcc atgggtgcgc ctttcctccg ccgcctactg ggccgatcag
    22681 tggggctgcc gtctggcgga aatcggcgcg gccggacata tcaatgcgga ctccggtcac
    22741 ggcccatggc cgctcggttt gcgcctgttc gaatccctgc ggcgggcggc cggtgactgg
    22801 ccgctgggcc ggatcgatgg gaacgcgcag ccgcggggcg ggcggcatgg cgccctcgcc
    22861 cgcgtccgtc agctcacccg cttagcctcc ggacgggaaa cgggcggcgg cgcgccgtca
    22921 gaaccgtcgg ggcgcggcgg ctgacggccc ctgcccgttc agaacggccc atcgctgccc
    22981 gtttcggggc gcagggcgcc gcgcaccacg gcctcggaga gctccggtgc gcacagctcg
    23041 atgaagcggt aggcaaaacc gcggagaaag tggcctcggc gcacggcgat gcgcgtcacg
    23101 ttttcaccga agaggtggtc gctgggcagc atgcgcaggg cgcggtcgcg ctcggcgtcg
    23161 taggccatgg aggccacgat gccgatgccc aggcccagct ccacataggt cttgatcacg
    23221 tcggcgtcga gggccgacat gacgatgtcc ggcgtgaaac cggcctgggc gaaggtttcg
    23281 tcgatcttcg cacggccggt gaagccttcg tggtaggtga tgaggggatg ctcggccacg
    23341 gcttcgaggg tgagcggtgt gaccgcctcc agcgcatgcc cggccgggac gatcaccgcg
    23401 tggtgccagc cgtagtacgg gaaggacacg aagtcggaca cgccggccag ggcttcggtg
    23461 gcgatgccga tgtcggcgcg gccgtccagc agcatggcgg cgatctcgcc ggggctggcc
    23521 tgatgcagca tcaaatgcac cctggggtac gcctgcttga agcgcgtcac cacccgtggc
    23581 agggcgtagc gcgcctgggt gtgggtggtg gccacggtga gctggccgtg atccttgttg
    23641 ctgaactgct cggcgatgct cttgatgttg cgcgcgtcca tcagcatgcg gtccaccacc
    23701 gcagagagct cccgccccgg ctcggtgagc ccgagcaggc gcttgccctt gcggacaaac
    23761 agttccaggc ccagctcgtc ctccaggtcc ttgatgtgct tggagacacc cgactgggac
    23821 gtgaacaggg cgttggccac ctcggtcagg ttaaagttgc gccggaccgt ttcatgaatg
    23881 atgcgcagtt gctggaaatt catcagatca cccagttcgg tgccgcggct gcggcagcct
    23941 cctgacgttc gaacaccttg aggcgggacg gttgcaggcg cacaacctgg ccttcttcca
    24001 ggcccaggcg gtggacatcg tcgcggggca ggtccacttc gtaaagctgc ccgccggcgc
    24061 cattgagccc atccagttcg acgcgggcca ccgcgccgaa ggcgaggatg cgcgacaccc
    24121 gtgccggcac gccgtcggcg gcggccggat cgacgacgat ggtgagttcg tggggccggg
    24181 cgaaggcaag cacttcggcg ccctgcccca gctccacccg ctggcgcgac agcaggtcgt
    24241 cgcccacgtg cacggcctga ccgtccacgc ggccgtggaa gagattgacc gcgcccagga
    24301 aaccatacac gaagggcgag gcgggatggc gatagacctc gtccggggtg cccacctgct
    24361 ccaccttgcc gtggtccatc agcaccaccc ggtcggccac ttcgagggct tcttcctggt
    24421 cgtgggtgac gaacagggag gtgatgtgca gctcgtcgtg gagccgccgc agccagcggc
    24481 gcagttcctt gcggaccttg gcgtcgaggg cgccgaaggg ttcgtccagg agcagcacgc
    24541 gcggctccac ggccagggcg cgggccaggg cgatgcgctg gcgctggccg ccggaaagct
    24601 ggctggggaa gcggtcggcg acccagtcga gctgcacgag gcccagcagt tccttgacct
    24661 tggcgcggat cgtcgcgtcc gaagggcgct ggccgcgggg cttcatgcgc aggccgaagg
    24721 ccacgttgtc gaagacggtc atgtggcgga acagggcgta atgctggaac acaaagccga
    24781 cctgacgctc gcgcacgtgg gtgtgggagg cgtcctgccc gtccagcagc acctggccgc
    24841 tgtcggccgc ttcgagcccg gcgatgatgc gcagcagcgt ggtcttgccg cagccggagg
    24901 ggcccagcag ggccaccagc tggccagtgg ggaagtcgag gctgacgtcg tgcagggcgg
    24961 tgaagctgcc gaactgcttg tggatgttgc ggacctggat gctcatttca ttcttccttg
    25021 ttagcccggc cggcctgacg ctcgacccag gtcttgatga ccagggtgac gagggccagc
    25081 agcgccagca gggaggccac cgcaaaggcg gcggcgaact ggtattcgtt gtagaggatc
    25141 tccacgtgca ggggcagggt gttggtctgt ccgcggatgt gcccggagac gacgctgacg
    25201 gcaccgaatt cgcccatggc gcgggcgttg cagaggatca cgccgtacag gagcccccac
    25261 ttcacgttgg gcagggtcac gtgccagaag gtctgccagc cccgcgcgcc gagcacgacg
    25321 gcggcttcct cttcctcacg gccctgggct tccatcagcg ggatcagttc ccgcgcgacg
    25381 aaggggaagg tgacaaaaac cgtggccagc acgatgcccg ggatggcgaa gatgaccttc
    25441 acgtcgtgat ccgacagcca cggcccaaac cagccctggg caccgaagac gagcacgtag
    25501 atcaggcccg agaccaccgg cgacacggag aacggcaggt cgatgagggt gatgagcagg
    25561 tgcttgccgc ggaactcgaa cttggtgatg gcccacgcgg cggccacgcc gaagacgaga
    25621 ttgaggggca ccgcacacag cgcggcagtc agggtcaggg tgatcgcgga caccgcatcc
    25681 ggctcgatca aggccgcgta atagaccgcc cagcccttgc gtagcgcttc gtagaacacc
    25741 gccaccaggg gcagcagcag gaagaccgca aagaaggcca gggcaaggcc gatgagggac
    25801 catttgaccc aggccggttc gcgcgtggcg gacctggatt cgaagcgtgc cgcatggtcg
    25861 aagacgccat gcagcgcagc cgcagcgcct gacatcagcg ggccctcccg gtgcgttggg
    25921 cggtccaggc ctgcaggccg ttgatgatca gcagcaggat gaaggagaag atcagcatga
    25981 ccaccgcgat cgccgttgcg ccggcgtagt cgtactgctc aagcttggtg atgatcataa
    26041 gcggagtgat ttctgacacg agggggatgt tgccggcgat gaagatcacc gagccgtatt
    26101 cgccgacggc gcgggcgaag gccatggcga agccggtcag gagggccggg aagacggtgg
    26161 gcaggaccac atgcaggaag gcctgccagc ggttggcgcc gaggctgacg gcggcctctt
    26221 cgtattcggt gtccaggtct tcgaggatgg gctgcacggt gcgcacgacg aagggcaggc
    26281 cgatgaagat gagcgccacc agcaccccca ggggcgtgaa ggccacctga atgccccagg
    26341 gttcgaggat ctgcccgacc cagccgttct tggcgtagag ggccgtaagg gcgatgcccg
    26401 ccaccgcggt gggcagggcg aagggcagat ccaccagggc gtccaccagg cgcttgccgg
    26461 ggaaggcata gcgcaccagc acccacgcca ggagcaggcc gaagacggcg ttgatcaacg
    26521 cagcgatgag ggaggcgccg aaggacaggc ggtaggaggc gagcacccgc ggcgaggtgg
    26581 cggcctgcca gaagtgttca aagccaaggg aaccggcctt gaggaacacc gccgccaggg
    26641 ggatcagcac gatcagggac aagtaggcga cggtgtaacc cagactcagg ccaaatccgg
    26701 gcagtacacg aagggatcgg gcggcgctca tcggtgtttc actccgacct ggtcgtacac
    26761 gccgccatcg gcgaagtgga ccttctgcac cgcatcccag ccgcccaggg cgtcctcgac
    26821 ggtgaaggtc cggatgctcg gaaaacggcg ggcgtagtgc ttgagcacct ggggatcgcg
    26881 gggccggaaa tggtgacggg cgatgatgtc ctgtccttcc ggggagaaca ggaagttcag
    26941 gtaagcctcg gccgccttgc ggctgccccg cttgtcgacc accttgcgaa ccacggccac
    27001 cggggcggcg gcctcgatgg agacggacgg atagaccacg tcgaacttgt cgccccccac
    27061 ttcctggtcg atgagggtgg cctcgttctc gaaggtgacc agcacgtcgc ccacgtcccg
    27121 ttgggtgaag gtggtggtgg cgccgcgccc gccgccgtcg agcaccggga cgttggcgaa
    27181 cagacggctg acgaaatccc gcgcgccggc ttcgttcccg gttttctgga gcgcataacc
    27241 ccaggcggcc agataagtgt agcggccgtt gccagaggtc ttggggttgg gcacgatcac
    27301 ctgcaggccc ggccgggtga ggtcggacca atccctgatg cccttggggt tgcccttgcg
    27361 cacgaggaag atggtggtgg tggtgtaagg cgtggcgcca tggggatagg ccttgcgcca
    27421 gtcgccggcg acgaggcccg cgcgggccag cacgtcgatg tccggtgcct ggttcatggt
    27481 gaccacgtcg gcctccagcc cggccacaac agactggacc tgcttgctgg agccgccgtg
    27541 ggactggttg atcgtgagct tctcgcccgt ttggcgctgc cattgggcgg caaaggcggg
    27601 gttgatgtcc ttgtacagct cgcgggagac gtcgtaggag acgttcagca gggtcgtgtc
    27661 ggcgtgggcc gcggtcacgc cgaggagtgc cgagctggcc atcagcgcac gccacgtacg
    27721 gcggctcaat cggatcgaag gaaattggaa cacggcgact cctggggttc tgatgatttc
    27781 ccggaagtct aagcagcacc cgtcggagcg ctaaccaata aatcctgcgt tctttattct
    27841 ctggggcgat aaagaatgga aattttaatt ctccgctgtt cttttccggc accattcagc
    27901 gtgccatcgc ctcaagcgtc tgcacctccg gacgcccaga gccccggtcg gtttcccgac
    27961 cggggctctg ggtgaaccag gttcatgaga caggaggcga cgagatcttc cagaatcatc
    28021 cggtgggtct tgccgtaagt gccaagtgaa aaaggccccg ccggtttccc ggcggggcct
    28081 ttttcgtatt gctacggtcg accggcaaaa gccggccatg atcgcaactt acatttcaac
    28141 cgtttcagcc acttccacga aggccgggat ctggtcgaag ttcatgtagc gatacacgtc
    28201 ggcagccttg gcgttcacca gcttgatctg ctccatgtac tcttccttgg tcacgatacg
    28261 gccggccagg gcgcacatgg cggccagttc ggcggagccg aggtagacgc gggtgtcgat
    28321 gcccagacgg ttcgggaagt tacgggtaga ggtggagatc gcggtggaac ccttgcggat
    28381 ctgcgcctgg ttgcccatgc acagggagca gcccggcatt tccatgcggg cgccggcctt
    28441 gccgaggacg ctgtagtagc cttcttcggt caggatcagg gcgtccatct tggtcggcgg
    28501 ggcgatccac aggcgggccg ggatgtcgct cttgccgtcc agcaccttgc cggccgcacg
    28561 gaagtggccg atgttggtca tgcaggagcc gatgaagact tcgtcgatct tgtcgccggc
    28621 gacttcggac agcaccttga cgtcgtccgg gtcgttcggg caggccagga tcggctcggt
    28681 gacttcggcc aggtcgattt cgatgactgc ggcgtactgg gcgttggcgt cacccttcag
    28741 caggacgccg ttggcgatcc agtcttccat ggcggcgatg cggcgcttga gggtgcgggg
    28801 atcctcgtag ccttccgcga tcatccactt catcagggtg atgttggaac gcatgtactc
    28861 gacgatcggc tccttgttga gggcgatcgc acaggcagcg gcggaacgct cggcagcggc
    28921 gtcggacagt tcgaaggcct gttcgacctt cagatccggc agaccttcga tttcgaggat
    28981 gcggccggag aagatgttct tcttgccctt cttctcgacg gtcagcaggc cggccttgat
    29041 ggcccacagg gggatggcgt tgaccagatc acgcagggtg atgccgggct gcatctggcc
    29101 cttgaagcgc accagcacgg attcgggcat gtccagcggc atcacgccgg tggcggccgc
    29161 gaaggcgacc agaccggagc cggccgggaa ggagatgccg atcgggaagc gggtgtggga
    29221 gtcgccaccg gtgccgacgg tatccggcag cagcagacgg ttgagccagg agtggatcac
    29281 gccgtcgccc ggacgcaggg ccacaccgcc gcggttggcg atgaaggtcg gcagttcgcg
    29341 gtgcatcttg acgtctacca gcttgggata ggccgcggtg tggcagaagg actgcatgac
    29401 catgtcggcg gagaagccga ggcaggccag atccttcagt tcgtcgcggg tcatcgggcc
    29461 ggtggtgtcc tgggagccga cggtggtcat cttgggctcg cagtaggtgc ccggcaggat
    29521 gcccttgcct tccggcaggc cacacgcgcg gccgaccatc ttctgggcca gggagtaacc
    29581 ggtgcccgga tcgttgggct gctggggcag gcggaacagg gtggagaccg gcaggcccag
    29641 ggcttcacgg gccttggagg tgagaccgcg accgatgatc agggggatgc ggccacc
//
LOCUS       DAOQPA010000032        29693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_698_length_29693_cov_18.500742, whole genome shotgun sequence.
ACCESSION   DAOQPA010000032 DAOQPA010000000
VERSION     DAOQPA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29693
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_29693_cov_18.500742"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKX01_06520"
     CDS             complement(<1..650)
                     /locus_tag="PKX01_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMZ75815.1"
                     /translation="MRALVIAEHDHGSLKAATLNTVTAAAKLGAPVDVLVAGAACAAA
                     ADAATKLAGVSTVKVADAPAYADQGAENLAALLVDVIKTGGYSHVLAPATTLGKNVLP
                     RVAALLDVAQISDIVAIESADTFVRPIYAGNALATVKSADAIKVVTVRTTAFDAVEPG
                     GSATLEAVAAGPDLGLSSLVGRELTVSARPELGAAKIIVSGGRGLGSGDAYRSVLEP"
     gene            complement(653..1402)
                     /locus_tag="PKX01_06525"
     CDS             complement(653..1402)
                     /locus_tag="PKX01_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMZ75816.1"
                     /translation="MKILVPVKRVVDYNVKIRVKADQSGVELANVKMAMNPFDEIAVE
                     EAVRLKEAQKATEVVAFSAGVAQCQETLRTAMAIGADRGILAETDADLQPLAVAKLLK
                     AVVAKEQPQLVILGKQAIDDDANQTGQMLAALLGWPQATFASKVELGADRVTVTREID
                     GGLETLSLPLPAVITTDLRLNEPRYATLPNIMKAKKKPLETVKPADLGVDVAPRLATL
                     KVVEPAARSAGVMVGDVAQLVNKLKNEAKVI"
     gene            1832..3874
                     /locus_tag="PKX01_06530"
     CDS             1832..3874
                     /locus_tag="PKX01_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HMZ75817.1"
                     /translation="MNALDDSAAAGDAAQSGAEGDRRYREMLGAGLDLLDQGVTVFDA
                     DLRMVAWNAAFLRLLEFPAELARPGVSFDDFIRYNARRGEYGPGDPEAQVAERVTRAR
                     SFQAHTTERVRPNGQVLLIRGAPLPHRGFITLYTDITEERRAQAELEEHIRVRTEALT
                     RANAELTAAVESNKAIAAALQRSEQRLREITDAIPAAVAYFDQNWTYRYANKGYAEWF
                     GWSAERIEGRSIREVIGQEVFDIVSPYVTRALAGERMAYEYTLTGRDGGPAHARSTLV
                     PDFGADGQVIGCYVHSVDITEPRRTQMALSQAQKMEAIGQLTGGLAHDFNNMLTVVMG
                     NLAALRENHPQDPLTENFVEPAMQAAHRGAELIRRLLSFARKQPLEARPVEVNELILG
                     MSKLIRRSLPGTIAVSTESREPCLVALVDEHQLENALLNLALNARDAMPNGGELRIES
                     SLETLSPSAAADLEVPPGPYVQIAVTDNGMGMDGSTLARVFEPFFTTKQFGMGSGLGM
                     SMVYGFVKQSGGGVRIRSRQARGTTVALLLPWVEDTADSEVVPVGAPVSEADLAGKLV
                     LLAEDDADVRTVVRMQLAELGCAVVEAENGAEAADMVENIPAIALVISDVVMPGDMDG
                     RALARFVRRFRPELPVVLMSGFADSAAAFDADLALPLLAKPFTRDKLLAALGPLAA"
     gene            3888..4601
                     /locus_tag="PKX01_06535"
     CDS             3888..4601
                     /locus_tag="PKX01_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ75818.1"
                     /translation="MTRNPELIYVVEDELDVARLIRAALEKFGFRTEVFRTAEAFFHG
                     VRHRVPDLVILDLGLPDMDGMAVLQQLRSKHTCGLLVLTGRGDVYDRVMGLELGADDY
                     VVKPYEPREMIARVRSILRRYPRQPVVPADPTQRVAEFAGWRFETGSNTLVGPDGQQE
                     TLSTAEAQLLSVLLAHPNHILTREQLLGGRDVSALDRSIDLRISRLRRRFEENPQHAK
                     LIKTVYGAGYLLAATVTWS"
     gene            4694..5164
                     /locus_tag="PKX01_06540"
     CDS             4694..5164
                     /locus_tag="PKX01_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HMZ75819.1"
                     /translation="MRHARMLALLVGTMMMAGAAMAADVEVRDAWVRGVVPAQKSTGA
                     FMQLTSPAGATLVGAASPAAAKVELHEMAMEGNVMRMRPVPRLALAPGQTVALKPGGY
                     HVMLIGLKKPLVDGDKVPLTLLLEGRDKQVDKVEVSAEVRPLTAAAPMDEHHHH"
     gene            complement(5195..5572)
                     /locus_tag="PKX01_06545"
     CDS             complement(5195..5572)
                     /locus_tag="PKX01_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75820.1"
                     /translation="MSTEALAARLAAWAAVTPLTPSDVDCTTTVMLKILDGKCKMSPT
                     DKVQMAALYDLLKDRPGARFGADFHALIARAREGLDEALRMEVYEQRLLAETRLSRPV
                     MKAYKAMLRATGALEAATADAED"
     gene            complement(5562..7259)
                     /locus_tag="PKX01_06550"
     CDS             complement(5562..7259)
                     /locus_tag="PKX01_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ75821.1"
                     /translation="MKLHLERPHVEALVGAFPRLAWLQEQLRFGHRAEVGLLQLEADE
                     LRFVETLLAGAGPEQHRRAAQIATVRKALGAGGVRFEAEDLEMMVPVLARYLAEDALH
                     GWLFSLTGPDRPTAWVVTRLDFTPAGNEEVGKLLIELKANARGKVITTNLRILAGDIA
                     GRTVGEILIAKGYLKETPELVAAYEEMAERYFDWRARYGEQFSGRGTAIFAENPTATH
                     RDTDWTRKEQVILSSSGGPARLVNDEGILADRELTLAAPGDILGPYLRRAAKSLRYKA
                     DDETEATRDTLPAGLFTRLPVHFFILMFHLDLHHYLWVHVADVTPYAYTPALKQKLIL
                     PEEQTDLIDILTAEMDLLTEDIVAGKSGGTTVLCAGPPGVGKTLTAEVYAEIIRRPLY
                     RVHSGQLGLNVAAMETALKDVLTRAQRWGAVMLIDEADVYIKRRDDNITMNAVVGVFL
                     RVLEYFNGLLFLTTNRIDDIDEAIVSRCIALIKYAPPDAEARSRIWQVMGEQFGLDLD
                     AAHIARLVALFPDATGRDIKGLAKLVAKYCARKACPADLDAFIRCATFRGLEVAHVH"
     gene            complement(7376..8275)
                     /locus_tag="PKX01_06555"
     CDS             complement(7376..8275)
                     /locus_tag="PKX01_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ75822.1"
                     /translation="MHYDLTDLRLFAALAEEASLTRGAARAHLAPSSASHRLARLEES
                     LGVALFERHARGLRPTRAGESLLGHARQVFAQLEQMHADLSPFARGVRGHVSLYANTN
                     AINCFLPDDLGEFLRGSPQIRITLKEMTSPEILRCVAAGEAEIGVVAGEIPPDSLIAH
                     PYRQDRIVLVTPPGHPLSARARVRISEALDQPFVMLHAGSAFHTGLMNYVAETGGRMD
                     VRIEVRSFEAVLRLAGAGVGLGLVPRSSAERIGRQRPFAIVDVDEPWAQRDLTIVVRR
                     DVALSPHADALVEALRHGGVSAS"
     gene            8375..9271
                     /locus_tag="PKX01_06560"
     CDS             8375..9271
                     /locus_tag="PKX01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ75823.1"
                     /translation="MSAAGHRLGVLFATLGAVGFSFKAILIKLAYPYGVDAVTLLALR
                     MGFSLPFFVAIGWADARRRQPAALGGRDVLLLLGLGTSGYYLASYLDFLGLQYISAAL
                     ERLILFSYPTLVVVFSAIFLGKRITGRALASIALCYAGIGLAVDHDLKVAGSGRDVWI
                     GGGLVFASAVSYALYLMGNGQAVARLGAARVTAWASTAACLLCLVHFGLTRPLGLLVQ
                     PAQVLGMAAAMAIFSTVLPVWLVSEAMRRLGAGPVSLIGTLGPVVTLFLGWLILAEPI
                     GVFQIAGGTLVVAGVLLVGKRG"
     gene            9376..10470
                     /gene="trmA"
                     /locus_tag="PKX01_06565"
     CDS             9376..10470
                     /gene="trmA"
                     /locus_tag="PKX01_06565"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HMZ75824.1"
                     /translation="MPLPRFDPADYDAQLAAKVARLRADFAPFGLPEPAVFRSEPLHY
                     RLRAEFRLWHREGRVDYAMFDPENPRQPVILDDFPAAAEPIHAAMPRLRALLCASEVL
                     RKRIFQVEFLATLSGELMISLVYHRPLDEAWTTAARALAAELGAQIIGRSRGQKIVLE
                     RDWLLEDFEVDGLRRRYQQIEGSFTQPNGGVNRQMLAWARARVAGVGGDLLELYCGNG
                     NFTLALAPCFERVLATELSKSSVHAARYNIALNDVANVTLVRMSSDEISDALAGGRAY
                     TRLRDVDLAGYRFSTLFVDPPRSGLDPATVALARGFGRILYISCNPQTLRDNVAALSA
                     THAIEAAAAFDQFPYTRHLECGVMLRRRAD"
     gene            10497..10838
                     /locus_tag="PKX01_06570"
     CDS             10497..10838
                     /locus_tag="PKX01_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HMZ75825.1"
                     /translation="MSALPPCPQCGSEFTYEDGAMLVCPECAHEWSASAAPEGGETAR
                     VVKDAHGTVLQDGDSVTVIKDLKVKGSSSVVKVGTKVKNIRLVDGDHDIDCRIDGFGA
                     MQLKSEFVKKA"
     gene            complement(10886..11266)
                     /locus_tag="PKX01_06575"
     CDS             complement(10886..11266)
                     /locus_tag="PKX01_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75826.1"
                     /translation="MPRDLPLPDTITDRGLRVALIFALAGERLNQFLEHGRWLTDAQG
                     ATLAADWLRRGRRTLERAEREHLSALSDRLARQVADSVSREAALYIAHEMAEALDPNY
                     VSDIARSLMAECERLLDADAAAGA"
     gene            11420..11674
                     /locus_tag="PKX01_06580"
     CDS             11420..11674
                     /locus_tag="PKX01_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75827.1"
                     /translation="MNTTCDCRGLPVAPGDTVRVLAVTLDPDLDDDDADMFRDMIGAT
                     CEVERIDGDGAAWVAVWWNGFDGPLLTTVGLDPAQMEKMA"
     gene            complement(11783..12517)
                     /locus_tag="PKX01_06585"
     CDS             complement(11783..12517)
                     /locus_tag="PKX01_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HMZ75828.1"
                     /translation="METATAPISREVALRIALAARAMPNATLPRLIELLQRQLGEEIQ
                     LDDLRKVTVTQLKTGFASADGEEDGEDIGIGLEAMKLAVRILWGETQGDDSLPRIQPY
                     TEGDMPGSVRVAIASDQGDTLSGHFGSCLRFLVYQVSPDEIRLVDIRDTMDAEFAPDK
                     NLWRAQLIGDCQVAYVVSIGGPAAAKVIRADIYPIKLPDGGKAEEVLVRFQQAMRESP
                     PPWLLKILGVSAEKRLKNYDASAADE"
     gene            complement(12663..14174)
                     /locus_tag="PKX01_06590"
     CDS             complement(12663..14174)
                     /locus_tag="PKX01_06590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ75829.1"
                     /translation="MTAAQRLRELLLPLVAGFGALALTLVLWQHEQQANQAAMAAHFD
                     ASVRQTAARIDQRIAVYTQMLRGVQGFFRASDLVNREDFATYVDALAQGPEFAGLQSL
                     VYSRAVPGPDGLHAPMIYAAPDLTGNQRAVGHDQMIDAARRAALLHARDSGTLAISQR
                     LRLHSDTQDGPESAFILYLALYRHDRPLNTQAERRDALTGWVQAAFRMKDLMSSLAGE
                     TPPGLDVQLYDGIEPRPDALLYDSRGGQDAAAPVRFEGREFVAAAGHDWCLVVRSQPE
                     FERRFGSKAAEIVLIAGISLSALIAALIWQLITCQTRAHAQARSMTQELRENAEVMRL
                     MAQHDPLTLLPNRALFSDRLQSVLARARRDTTRAALMFVDLDRFKPVNDAYGHAVGDD
                     LLREVSRRMKLCLRESDTLARLGGDEFVVLLAPVDAADDARIVAQRILQTVEEPFYIA
                     DHRLSVSASIGIALYPDQGVDDLALMKAADDAMYVAKDRGRGRMVFAGDPAPA"
     gene            complement(14176..14955)
                     /locus_tag="PKX01_06595"
     CDS             complement(14176..14955)
                     /locus_tag="PKX01_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ75830.1"
                     /translation="MLLYCAFVCLMLCLTRPAAADGPDCTRPRTLALHEHGLLYSGAS
                     GEGIDKDFADELIRRSGCPVNVTVLPRARIWQLIETGALDFSLSGITNDARERFAAFA
                     WYFSNRYTLLVRKDAGVTRIDELDARPALRLGAIRSFRYGPTANRLVDRLAQTGRVIH
                     ANTLDTLYRALATDHIQAMIIEPFDIPILERARLAEQTRTLEFPDPPIPHGLIMSRAA
                     VSPEEQARWRALVDGMRADGSVERIFGKYFKPDLARAMTRF"
     assembly_gap    15129..15228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15229..17038)
                     /locus_tag="PKX01_06600"
     CDS             complement(<15229..17038)
                     /locus_tag="PKX01_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HMZ75831.1"
                     /translation="MRRSPSGPPPAPATGDRHDWQTIRQLLPYLWAYKGRVLFALVCL
                     ISAKFANVSVPLIFKRLVDDLDLSRGQALIVVPAALLLAYGVLRFSTSLFTELREIIF
                     ARVTQEAVREISLQVFHHLHALSLRFHLERQTGGLTRDIERGTRSIGSLISYTLYSIL
                     PTLVEIGLVMGILLTKYEPSFAAITAATLTCYIVFTVRVTHWRTALRRRVNELDSAAN
                     ARAIDSLINFETVKYFNNEAFETRRYDAELARWTEAQIRNQQSLSLLNMGQAAIIAVG
                     VTAMMWRAALGVAGGQMTLGDLVLVNAFLIQLYVPLNFLGVLYREIRQALTDIERMFG
                     LLDARREVADRTDARALARGPAEVRFDHVAFAYEPARAILHQVDFAIPAGHTVAVVGH
                     SGSGKSTLARLLFRFYDVQGGAVRINGEDIRALTQDSLRAAIGIVPQDTVLFNDTLYY
                     NVQYGRPDAPRAEIEAAARAAQLEDFIARLPEGWETRVGERGLKLSGGEKQRVAIART
                     LLKNPDILIFDEATSALDSKTEKAIQAQLDAAARGRTTLVIAHRLSTVMNADEILVLD
                     QGHIVERGDHASLLARNGAYAQMWALQQEERAAEEAA"
     gene            complement(17084..17716)
                     /locus_tag="PKX01_06605"
     CDS             complement(17084..17716)
                     /locus_tag="PKX01_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HMZ75832.1"
                     /translation="MPSFDTLLAFLGLSIALTLAPGPDNLMVLGQALAQGWRAGLAIA
                     LGCALGCLTHLTWTVLGVAALLAASPRAFFALRVAGAAWLLGLAVQALRGSRPVRAGD
                     AAAVPWRRSLRRGFVANALNPKVALFFLAFLPQFVDPARGDTAHQMIVLGLVFSAQTV
                     VVFGGIALASGALGHALARHPALGPWLDRLCGLIFAGIALRLLAEPSAGA"
     gene            complement(17792..18922)
                     /locus_tag="PKX01_06610"
     CDS             complement(17792..18922)
                     /locus_tag="PKX01_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75833.1"
                     /translation="MWIARHRFEDMQRRLDGAQRQESALNAEIDALRAQLAARAAPSP
                     QDNEAAMLRAVITRMAEFGTSLTDAQGSLGEMARLLRDEKEKAVEAAHVSLASGQATT
                     EIAANLQRLADESSQSAREVDTLAQRADEIGTIVKLIHGVADQTNLLALNAAIEAARA
                     GEAGRGFAVVADEVRKLAERTSTATNEISALVNRIREDSARARSAMESLSRAAGEFSA
                     RGQLATADMQRLMTLSGNMEGVIAGSAMKSFVEVAKIDHIVFKFRIYLAVFGLQPLDA
                     GALPDHTACRLGKWYYTGEGQACFSRLPGYREVEPPHIEVHRAGADVLRALTAGNTVD
                     LVRQLDRMEQASLRVLDHLQRMGEAAVGDPSVLCHAGDGHAH"
     gene            19103..19720
                     /locus_tag="PKX01_06615"
     CDS             19103..19720
                     /locus_tag="PKX01_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HMZ75834.1"
                     /translation="MNCRDERLPAALLGLVALVLAVSAIAPYDRATWWMEVAPVFLVA
                     PLLIASHRRFPLTPLLQTLIALHALVLIVGGVWTYARVPFGFWLQDVFHLARNPYDRI
                     GHFMQGFVPALAAREVLLRRGIVRGRGWLFFLVTCVCLAVSALYELIEWGAAVALGQG
                     ADAFLGTQGDPWDTQNDMAMALVGALVAQIGLGGWHARQIARLRL"
     gene            19849..21456
                     /locus_tag="PKX01_06620"
     CDS             19849..21456
                     /locus_tag="PKX01_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HMZ75835.1"
                     /translation="MPDLPVMPPLEAAPAEPPAAPITPADLVLAYHRRTRHRFDAYAR
                     GPSTLDWDAQPAPFRHFGGTRRIRLPLACAEACAAPPATPAAADLTALGTLLHLSFGI
                     TAWKRLGPDRWAVRANPSSGNLHPVEAYVLVAGLPDLEDGLYHYLPDVHALELRAAHP
                     RPFAAGPALAVGLTSVMWREMWKYGERAFRYCQLDTGHALACLHYAAALLGWRVSDRP
                     GLASAALARVLGTDRDEDFPGRRPDVSREEVELLACVGPADGLPVVTADALAEAADAA
                     RWQGVASEVDRHPMFRWPVVDEVAAASRRVSGVPVTPLPVAIPRPASARHILQRRSAQ
                     RFDPAHVMPREDFVRLLDALRPVAGGRLGLALFIHRVDGLAPGVYLLARADWLRPQPT
                     RAGASALQPVTGCPDLYCLSTSESVPLRRLARSLHCHQDIAANACIAFGLLAQLEREV
                     ADDPARYRDLLREAGFLGQVLYLEAEAMGLRGTGVGCYFDDPVHELVIAGQRDAHSLY
                     HFTIGLPVDDGRIETGPAYPATLEMPS"
     gene            21453..22277
                     /locus_tag="PKX01_06625"
     CDS             21453..22277
                     /locus_tag="PKX01_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ75836.1"
                     /translation="MNASATAPLSAKFQRISAAEAAALLRRPEGAPRPVLFDVRDRAS
                     YERAHVSGAEHLTDAHLGAALRRVPKSAAVMIYCYHGNASQVYAETFADFRFAEVYSV
                     DGGYEPLAAALALLDGVRLAPRLPDGASADLRAFVLRHAFDPADLDAPRAQGLTPLMR
                     AALQGQAELVGELLAAGVEVDRRNGDGNNALWLACVSRDAATVAALVAAGIDLDNRND
                     MGATCLMYAASSGRPTMVEALLKAGADPYVRNFDDARAVDLCATLECLRLLRHTGN"
     gene            22314..22634
                     /locus_tag="PKX01_06630"
     CDS             22314..22634
                     /locus_tag="PKX01_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75837.1"
                     /translation="MSKYRSIALLMEAVEAPLAAAGVVAGEAWRADEQAVGFFSHADV
                     GLAAYVFTYGQRSGRYGINLDFPLPAEGGPLLTVPTSAEDLPLAAVIALLLEHFDVPV
                     LEAA"
     gene            complement(22842..23228)
                     /gene="apaG"
                     /locus_tag="PKX01_06635"
     CDS             complement(22842..23228)
                     /gene="apaG"
                     /locus_tag="PKX01_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMZ75838.1"
                     /translation="MTTLPDPDSIQVKAVARYLADQSDEAADRYVFAYHITIRNTGDA
                     PAQLLSRHWIITDAHGTIQEVEGSGVIGQQPTLKPGEAFEYTSGCVLTTPVGTMKGSY
                     RMQTEDGVDFNATIPEFTLAMPRTLH"
     gene            23365..24057
                     /gene="rpe"
                     /locus_tag="PKX01_06640"
     CDS             23365..24057
                     /gene="rpe"
                     /locus_tag="PKX01_06640"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMZ75839.1"
                     /translation="MSFRIAPSLLSANFAQLGAEVANVIAAGADWIHFDVMDNHYVPN
                     LTIGPLVCEAIRPCTTAPIDVHLMVKPVDRIVPDFAKAGADIITFHPEASEHIDRTLG
                     LIRDSGCQAGLVFNPATPLHWMDHVMDKLDVVLLMSVNPGFGGQSFIPGTLDKLREAR
                     AKIDAYTARSGRPILLEIDGGVKVDNIAEIARAGADTFVAGSAVFGAGRDTDPHRYDS
                     VIGALRSALAGV"
     gene            24059..24712
                     /locus_tag="PKX01_06645"
     CDS             24059..24712
                     /locus_tag="PKX01_06645"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HMZ75840.1"
                     /translation="MSPRHAVLFDLDGTLLDSIPDLAEACQRMMRELGHPPHDLETIR
                     SFVGKGMVNLVTRCLGDASAEAVDAAVAVFRRHYAAVNGDATTLYPGVVPALEALAAR
                     GVPLACVTNKPAAFTEPLLARCGIADYFAVTVSGDTLAEKKPHPAPLQHACRLLGVAP
                     AAALMVGDSANDAEAARAAGMPVLLVTYGYSEGRPVDSIDCDGLLSSLEDVLRHLPA"
     gene            24906..26378
                     /gene="trpE"
                     /locus_tag="PKX01_06650"
     CDS             24906..26378
                     /gene="trpE"
                     /locus_tag="PKX01_06650"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HMZ75841.1"
                     /translation="MTEAEFNALAAQGYNRIPVTVDTFADLDTPLSVYLKLANAPNTY
                     LLESVQGGERFGRYSIIGLACSTRIEARGRSVMLLTNDRVVERRDDTNPLDYVAEFKA
                     RIKVPPRDRLPRFAGGLVGCFGYDTVRYIEPRLASTHKPDALGTPDVLLLLSEEIAIV
                     DNLSGKLTLVVYAEPQVPDAYARATRRLKELIGRLREPVSIPAEAPGAPAEAVSSFGE
                     AAFKDAVRKAKHYIVEGDIMQVVLAQRMSKPFAASPLALYRAIRSLNPSPYMFYFNFE
                     DFQVVGASPEILVRLEDNVVTVRPIAGTRKRGATHDEDLALEAELLADEKERAEHLQL
                     LDLGRNDCGRVAGTGSVRVTEQFTVERYSHVMHIVSNVEGRLRDGLDALAVLRATFPA
                     GTVSGAPKLRAMEIIDELEPTKRGIYAGSVGYVGFHGDMDLAIAIRTAVLKDGHIHVQ
                     AGAGIVADSNPDAEWEETQNKARAMLRAAEIAEAGLDTPL"
     gene            complement(26483..27037)
                     /locus_tag="PKX01_06655"
     CDS             complement(26483..27037)
                     /locus_tag="PKX01_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75842.1"
                     /translation="MKNARILLTAVTLAAATAPAFAGDWKFLPILDKDYRPNFVASVT
                     GGMLDPKHGSSDTALGLELAMNCGLFQTPTGVVRTKLSWNRYDKDGVTLNTVELNPRW
                     TIPVAQNLTFGVGPGIGYVKADAGGRTTDLFAWQAGADLDYRIGQVNLGLGARWQDTV
                     NKDIAPGMQGAENWLVQAKVGIAF"
     gene            27223..27792
                     /locus_tag="PKX01_06660"
     CDS             27223..27792
                     /locus_tag="PKX01_06660"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMZ75843.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGADVRVFRNDEITVEQIAELAP
                     AQIVVSPGPCSPAEAGISVAAIQTFAGKIPLLGVCLGHQSIGAAFGGRIVHAKQLMHG
                     KVSPVHHLDQGVFKGLPNPLTCTRYHSLAIERETLPDCLEVTAWTDDGEIMGVRHKTL
                     AVEGVQFHPESILTERGHDMLRNFLDASR"
     gene            27805..28836
                     /gene="trpD"
                     /locus_tag="PKX01_06665"
     CDS             27805..28836
                     /gene="trpD"
                     /locus_tag="PKX01_06665"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HMZ75844.1"
                     /translation="MNFTPQEALQRTIEHREIFYDEMLSLMRQIMAGEVSPVMTAAIL
                     TGLRVKKETIGEIAAAATVMRELSSKVIVPPPHEHFLDVVGTGGDGAHTFNISTATIF
                     VAAAAGARVAKHGGRSVSSKSGSADVLEALGVNLNLNAEQVAECIARTGIGFMFAPNH
                     HSAMKNVAPVRREMGVRTLFNILGPLTNPAGAPNTLLGVFHADLVGICVRVMQRLGAH
                     HVLVVHGRDGMDEVSLGAATLVGELKDGEIREYEIHPEDFGLAMMSSRRLQVSTADES
                     RAMLLSVLDNVPSAAREIVILNAGVALYTANLADSIGAGIALAREAIASGAARARLDA
                     FVAATRSFA"
     gene            28844..29632
                     /gene="trpC"
                     /locus_tag="PKX01_06670"
     CDS             28844..29632
                     /gene="trpC"
                     /locus_tag="PKX01_06670"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HMZ75845.1"
                     /translation="MSDILNKILAVKRDEVAAARARVSDAAVRQLAADQPAPRDFVGS
                     MRARLGAGRSAVIAEIKKASPSKGVIRADFRPAEIAPSYERAGAACLSVLTDRQFFQG
                     APDYLQAARAACTLPVLRKDFLVDPYQVFEARAMGADAILLIVSALSLAEMQAMEAIA
                     ESLGMAVLVEVHDGAELDLALQLRTPLVGINNRNLRTFEVSLQTTLDLLPRIPAERIV
                     VTESGILQPADVALMREHRVNAFLVGEAFMRADDPGAGLAALFA"
BASE COUNT         4207 a        10250 c        10518 g         4618 t
ORIGIN      
        1 ggttcgagca cgctgcggta ggcgtcgccg ctgcccagac cgcggccgcc ggagacgatg
       61 atcttggccg cgcccagttc gggacgggcg gagacggtca gttcgcggcc caccaggctg
      121 ctgaggccca ggtcggggcc ggcggcgacg gcttccaggg tcgcgctgcc gcccggttcg
      181 acggcgtcga aggcggtggt gcgcacggtg acgaccttga tggcgtcggc gctcttgacg
      241 gtggccaggg cgttgccggc gtagatcggg cgcacgaagg tgtcggcgga ttcgatggcg
      301 acgatgtcgg agatctgggc gacgtcgagc agcgcggcga cgcggggcag gacgttcttg
      361 cccagggtgg tggcgggggc cagcacatgg ctgtagccgc cggtcttgat gacatccacc
      421 agcagcgcgg cgaggttctc ggcgccctgg tcggcgtagg cgggcgcgtc ggcgaccttg
      481 acggtgctga cgccggccag cttggtggcg gcgtcggcgg ccgcggcgca ggcggcgccg
      541 gccaccagca cgtccaccgg tgcgcccagc ttggcggcgg cggtgacggt gttgagggtg
      601 gcggccttga ggctgccgtg gtcgtgttcg gcgatgacga gtgcgcgcat gttcagatca
      661 ccttcgcttc gttcttgagt ttgttcacca gctgggccac gtcgcccacc atgacgccgg
      721 cgctgcgggc ggccggctcc accaccttga gggtggcgag gcgcggggcc acgtccacgc
      781 ccagatcggc cggcttgacg gtttccagcg gcttcttctt ggccttcatg atgttgggca
      841 gggtcgcgta gcgcggctcg ttgaggcgca ggtcggtggt gatcacggcc ggcagcggga
      901 gggagagggt ctccaggccg ccgtcgatct cacgggtgac ggtgacccgg tcggcgccca
      961 gctccacctt ggaggcaaag gtggcctgcg gccagccgag cagggcggcg agcatctggc
     1021 cggtctggtt ggcgtcgtcg tcgatggcct gcttgccgag gatcaccagc tggggctgct
     1081 ccttggcgac cacggccttg agcagcttgg cgacggcgag cggttgcagg tcggcgtcgg
     1141 tctcggccag gatgccccgg tcggcgccga tggccatggc ggtgcgcagg gtttcctggc
     1201 actgggcgac gccggcggag aaggcgacga cctcggtggc cttctgggct tccttgaggc
     1261 gcacggcctc ctcgacggcg atctcgtcga aggggttcat ggccatcttc acgttggcca
     1321 gctccacgcc gctctggtcg gccttgacgc ggatcttgac gttgtaatcg acgacccgtt
     1381 tgacgggcac gagaatcttc atggctgctt tttctccggt ggtgagcttt cagtccaatg
     1441 cggcgggcga tacgacgacg gcgcgcgggt gcgccggggg ctgcccatgg gccatgaaga
     1501 cggtggcgga gccgggtcgg acggtgcggg aggcgggggc ggacggcggc tctggcgcga
     1561 atggccggaa cggtccgggg tgatgcggga cgggggacat ccgattctcc tcctcgtggt
     1621 gcacggtcgc cgacgatcgg cgctgcggcg gctctgtggc ccttcttttt tccccggctc
     1681 gggacggacg cccggggatg gggacggagc ttggcaggag aacgtgtcgc aactttcgcc
     1741 aaaatgtact gaatcttgtc ggccgttccg gcgtgcggac ggcgggctag aatccgcccg
     1801 aaggcaggag gcgaaggggt tgatgggggc tatgaacgca ctggacgaca gcgcggccgc
     1861 gggcgacgcg gcgcagagtg gcgcagaagg ggaccggcgt taccgggaga tgctcggggc
     1921 cggtctggac ctgctcgacc agggcgtgac ggtcttcgac gccgatctgc gcatggtggc
     1981 gtggaacgcc gccttcctgc gcctgctgga gttccccgcc gagctggcgc ggcccggcgt
     2041 gtccttcgac gacttcatcc gctacaacgc ccgccgcggc gaatacgggc ccggcgatcc
     2101 ggaggcccag gtggcggagc gggtcacccg tgcccgcagc ttccaggccc acaccaccga
     2161 gcgggtgcgc ccgaacggcc aggtcctgct gatccgcggt gcgccgctgc cccaccgggg
     2221 cttcatcacg ctgtataccg acatcaccga ggagcgccgc gcccaggccg agctggagga
     2281 acacatccgc gtgcgcaccg aggccctgac ccgcgccaac gcggagctca ccgcggcggt
     2341 ggagtccaac aaggccatcg ccgccgcctt gcagcgcagc gagcagcgcc tgcgtgagat
     2401 caccgacgcg atccccgccg cggtggccta tttcgaccag aactggacct accgctatgc
     2461 caacaagggc tatgccgagt ggttcggctg gtcggcggag cgcatcgagg ggcgcagcat
     2521 ccgcgaggtg atcggccagg aagtgttcga catcgtctcg ccctatgtga cccgcgccct
     2581 ggccggcgag cgcatggcct acgaatacac cctgaccggc cgcgacggcg gaccggccca
     2641 tgcccgcagc accctggtgc cggatttcgg cgccgacggg caggttatcg gctgctatgt
     2701 gcattcggtg gacatcaccg agccgcggcg cacccagatg gcgctgtccc aggcccagaa
     2761 gatggaagcc atcggccagc tcaccggcgg gctggcccac gatttcaaca acatgctcac
     2821 ggtagtgatg ggcaacctgg ccgccctgcg ggagaaccat ccccaggacc ccctcaccga
     2881 aaacttcgtc gagcccgcca tgcaggccgc ccaccggggc gccgagctga tccggaggct
     2941 cctcagcttc gcccgcaagc agcccctgga ggcgcggccg gtggaggtca atgagctgat
     3001 cctgggcatg tccaagctga tccgccgctc cctgccgggg accatcgcgg tgagcacgga
     3061 gagccgtgag ccctgtctgg tggcgctggt ggacgagcac cagctcgaga acgccctcct
     3121 caacctggcc ctcaacgccc gtgacgccat gcccaacggc ggcgagctgc gcatcgagtc
     3181 gtccctggaa accctcagcc cctcggccgc cgccgacctg gaagtgccgc ccggccccta
     3241 cgtgcagatc gccgtcaccg ataacggcat gggcatggac ggcagcaccc tggcgcgggt
     3301 ctttgagccc ttcttcacca ccaagcagtt cggcatgggc agcggcctcg gcatgtccat
     3361 ggtgtacggc ttcgtcaagc agtccggtgg cggcgtgcgc atccgcagcc gtcaggcccg
     3421 cggcaccacg gtggcgctgc tgctgccctg ggtcgaggac acggccgaca gcgaggtggt
     3481 gccggtcggc gcgcccgtgt cggaggccga tctggcgggc aagctggtgc tgctggccga
     3541 ggacgacgcc gacgtgcgca ctgtggtgcg catgcagctc gccgagctgg gctgcgccgt
     3601 ggtggaggcg gagaacggcg ccgaggcggc ggacatggtg gagaacatcc ccgccatcgc
     3661 cctggtgatc tcggacgtgg tgatgcccgg cgacatggac ggccgcgccc tggcgcgctt
     3721 cgtgcgccgc ttccggcccg aactgccggt ggtgctgatg agcggcttcg ccgattcggc
     3781 ggccgccttc gatgcggacc tggccctgcc cctgctggcc aagcccttca cccgcgacaa
     3841 gctgctggcc gcccttggcc cgctggcggc ctgaggggag ggccgtcatg acccgcaacc
     3901 cggagctgat ctatgtggtc gaggacgaac tggatgtggc gcgcctgatc cgcgccgccc
     3961 tggagaagtt cggcttccgc accgaggtct tccgcaccgc cgaggccttc ttccacgggg
     4021 tacgccaccg ggtgccggac ctggtgatcc tcgacctggg cctgcccgac atggatggca
     4081 tggcggtgct ccagcagttg cgcagcaagc acacctgcgg cctgctggtg ctcaccgggc
     4141 ggggcgacgt ctacgaccgg gtgatgggcc tcgagctggg cgccgacgat tatgtggtga
     4201 aaccctacga gccccgcgag atgatcgccc gggtgcgcag catcctgcgc cgctacccgc
     4261 gtcagccggt cgtgccggcc gatcccaccc agcgggtcgc ggagtttgcc ggctggcgct
     4321 tcgagaccgg cagcaacacc ctggtcgggc ccgacggcca gcaggagacc ctcagcaccg
     4381 cggaggccca gctcctgagc gtgctcctgg cccatcccaa ccacatcctc acccgcgagc
     4441 agctcctggg cgggcgcgac gtgtccgcgc tggaccgcag catcgacctg cgcatttcgc
     4501 gcctgcgccg gcgtttcgag gaaaaccccc agcacgccaa gctcatcaag acggtgtacg
     4561 gcgccggtta cctgctggcc gccaccgtca cctggtcctg aggccgcctg cggcaacgcg
     4621 tcgcagggca tcgatcgccc gttgcggcgg gcgagccggt aagcttgcgc ctttgccaaa
     4681 ctggagcgac aggatgagac atgcccggat gctggccctg ctggtcggga cgatgatgat
     4741 ggccggcgcg gcgatggccg ccgatgtgga ggtgcgtgac gcctgggtgc ggggcgtggt
     4801 cccggcccag aagagcacgg gcgccttcat gcagctcacc agcccggccg gcgcgaccct
     4861 ggtgggtgcc gccagcccgg ccgcggccaa ggtggaactc cacgagatgg ccatggaagg
     4921 caatgtgatg cgcatgcgcc cggtgccccg cctggcgctg gcgcccggcc agaccgtggc
     4981 gctgaagccc ggcggctacc acgtgatgct gatcggcctg aagaagcccc tcgtcgacgg
     5041 tgacaaggtg ccgctcacgc tgctgctgga agggcgcgac aagcaggtcg acaaggtcga
     5101 ggtgagcgcc gaggtgcggc ccctcacggc ggcggcgccg atggacgagc accaccacca
     5161 ctgaacggca ggcccgccgg gtggcttggc gggttcagtc ctcggcgtcc gccgtggccg
     5221 cctccagcgc cccggtggct cgcagcatgg ccttgtaggc cttcatgacg gggcgcgaca
     5281 ggcgcgtctc ggcgagcagg cgctgctcgt agacctccat ccgcagggcc tcgtccaggc
     5341 cctcgcgggc gcgggcgatg agggcatgga agtcggcgcc gaagcgcgcc cccggccggt
     5401 ccttgagcag gtcgtagagg gcggccatct gcaccttgtc cgtcgggctc atcttgcact
     5461 tgccgtcgag gatcttgagc atcaccgtgg tggtgcagtc cacgtcggac ggggtgaggg
     5521 gcgtgactgc cgcccaggcg gccaggcgcg cggcgagggc ctcagtggac atgggccacc
     5581 tccaggccgc ggaaggtggc gcagcggatg aaggcgtcca ggtccgcggg gcaggccttg
     5641 cgggcgcagt acttggccac cagcttggcg agccccttga tgtcgcggcc ggtggcgtcc
     5701 gggaacaggg cgaccaggcg cgcgatatgg gccgcgtcca gatccagccc gaactgttcg
     5761 cccatcacct gccagatgcg gctgcgcgcc tcggcgtccg gcggggcgta cttgatcagg
     5821 gcgatgcagc ggctgacgat ggcctcgtcg atgtcgtcga tgcggttggt ggtgaggaag
     5881 agcaggccgt tgaagtattc cagcacccgc aggaacacgc ccaccacggc gttcatggtg
     5941 atgttgtcgt cgcgccgctt gatgtagacg tcggcctcgt cgatgagcat caccgcgccc
     6001 cagcgctggg cgcgggtgag cacgtccttg agggccgtct ccatggccgc cacgttgagg
     6061 ccgagctggc cggagtgcac ccggtagagg gggcggcgga tgatctcggc gtacacctcg
     6121 gcggtgaggg tcttgcccac gccggggggg ccggcgcaca gcaccgtggt gccgccggat
     6181 ttgccggcga cgatgtcctc ggtgaggagg tccatctccg cggtgaggat gtcgatgagg
     6241 tcggtctgct cctccggcag gatcagcttc tgcttgaggg ccggcgtgta ggcgtagggc
     6301 gtcacgtcgg ccacgtgcac ccacaggtag tggtgcaggt ccaggtggaa catgaggatg
     6361 aagaagtgca ccggcaggcg tgtgaacagg ccggcgggca gggtgtcgcg ggtggcctcg
     6421 gtctcgtcgt cggccttgta gcgcaggctc ttggcggcgc ggcgcaggta ggggccgagg
     6481 atgtcgcccg gcgcggcgag ggtcagctca cggtcggcga ggatgccttc gtcgttcacc
     6541 aggcgggccg ggccgccgct ggaggacagg atcacctgct ccttgcgcgt ccagtccgtg
     6601 tcgcgatggg tggcggtggg gttctcggcg aagatcgccg tgccccgccc ggagaactgc
     6661 tcgccgtagc gggcgcgcca gtcgaagtag cgctcggcca tctcttcgta ggcggccacc
     6721 agctccgggg tctccttgag gtagcccttg gcgatgagga tctcgcccac ggtgcgcccg
     6781 gcaatgtcac cggcgaggat gcgcaggttg gtggtgatca ccttgccgcg ggcgttggcc
     6841 ttgagctcga tgaggagctt gcccacttcc tcgttgccgg cgggggtgaa gtccaggcgc
     6901 gtgaccaccc acgcggtggg gcggtccggc ccggtgaggg agaacagcca gccgtgcagg
     6961 gcatcctccg ccaggtaacg ggcgaggacc ggcaccatca tctccaggtc ttccgcctcg
     7021 aagcgcacgc cgccggcgcc cagagccttg cgcacggtgg cgatctgggc ggcgcggcgg
     7081 tgctgttccg gcccggcgcc ggccagcagg gtctccacga aacgcagttc gtcggcttcc
     7141 agctgcagca ggccgacctc ggcgcggtgg ccgaaacgca gctgctcctg caaccaggcg
     7201 aggcggggga aggcccccac cagggcttcc acgtgggggc gttcgaggtg tagcttcatc
     7261 ggcaaggtct ccgcggcggt cggagccctt cacgcaaatt ccgcgccgcc tgccggacgc
     7321 acgcaagtga cgggggcaaa agggattttg cgccggagag gggtgcgacg gcgtgtcagg
     7381 aagccgacac gccaccgtgg cgcagggcct cgacgagggc gtcggcgtgg ggcgacaggg
     7441 ccacgtcgcg gcgcaccacg atggtcaggt cgcgctgggc ccagggctcg tccacatcca
     7501 cgatggcgaa ggggcgctgg cggccgatgc gctcggccga ggagcgcggc accaggccca
     7561 ggccgacacc cgcgccggcc agacgcagca ccgcctcgaa gctgcgcacc tcgatgcgca
     7621 cgtccatgcg cccgccggtc tcggccacgt agttcatcag cccggtgtgg aaggcgctgc
     7681 ccgcgtggag catcacgaag ggctggtcga gggcttccga gatgcgcacc cgcgcccgcg
     7741 ccgacagggg gtggccgggc ggcgtgacga gcacgatgcg gtcctgccgg taggggtggg
     7801 cgatcagcga atcgggcggg atctcgcccg ccaccacgcc gatctcggcc tcgccagcgg
     7861 ccacgcagcg caggatctcc gggctggtca tctccttgag ggtgatgcgg atctgaggcg
     7921 agccgcgcag gaactccccc aggtcgtcgg gcaggaagca gttgatcgca ttggtgttgg
     7981 cgtagaggct cacgtggccg cgcacgccgc gggcaaaggg actcaggtcg gcgtgcatct
     8041 gttcgagctg ggcgaacact tgcctggcgt ggccgagcag ggactcgccg gcgcgggtgg
     8101 ggcgcaggcc gcgggcgtgg cgttcgaaca gggccacgcc gaggctctcc tccaggcggg
     8161 ccaggcggtg actggctgag gacggtgcca ggtgggcgcg ggctgcgccg cgggtcaggc
     8221 tggcttcctc ggcgagggcc gcgaagaggc gcaggtcagt caggtcgtaa tgcacaaggt
     8281 ttcgtctcca tcgaatgctt ggctcggatc ttagcaattc ccaccgtagc cgggcgatgg
     8341 cctaatggtg gcctgttccc tgctccgtct tcccatgtcc gcagccggtc atcgcctcgg
     8401 cgtcctcttt gccaccctcg gtgccgtggg cttttcgttc aaggccatcc tcatcaagct
     8461 cgcctacccc tacggcgtgg atgcggtcac cctgcttgcc ctgcgcatgg gcttctcgct
     8521 gcccttcttc gtggcgatcg gctgggccga cgcgcgccgc cgtcagcccg cggccctggg
     8581 cgggcgtgat gtgctgctcc tgctggggct gggcacctcc ggctattacc tggcgagcta
     8641 cctggacttc ctcggcctgc aatacatctc tgcggcgctg gagcggctga tcctgttctc
     8701 ctatccgacc ctggtggtgg tgttctcggc gatcttcctg ggcaagcgca tcacgggccg
     8761 ggcgctggcg tccatcgccc tgtgttacgc gggcatcggc ctcgcggtgg accacgatct
     8821 gaaggtggcg ggctcggggc gcgatgtatg gatcggcggc gggctggtgt tcgccagcgc
     8881 cgtgtcctac gccctgtacc tgatgggcaa tgggcaggcg gtggcgcgcc tgggggcggc
     8941 gcgggtcact gcgtgggcca gcaccgcggc ctgcctgctg tgcctggtgc atttcgggct
     9001 gacccggccc ctcggcctgc tcgtccagcc ggcgcaagtg ctgggcatgg cggcggcgat
     9061 ggcgatcttc tccacggtgc tgccggtgtg gctggtgtcg gaggccatgc gtcgcctggg
     9121 ggccgggccg gtgtccctga tcggcaccct tgggccggtg gtgaccctgt tcctcggctg
     9181 gctgatcctg gcggagccga tcggggtctt ccagatcgcg gggggcacgc tggtggtggc
     9241 cggcgtgctc ctggtgggca agcgcggctg acgcgcgcgg ggtgcgcggg gggcgtgtcc
     9301 ggcgtatagt ccgcgcttcg ccacgcgtgc gggcctcttt ccgggcccgt accggcccgt
     9361 tttcgctgca tcgccatgcc tctgccccgt ttcgaccccg ccgactacga cgcccagctt
     9421 gccgccaagg tggcgcgctt gcgggccgat ttcgcgccct tcggcctgcc cgagccggcg
     9481 gtgttccgct ctgagcccct gcattaccgc ctgcgcgccg agttccgcct gtggcaccgg
     9541 gaggggcgcg tggattacgc catgttcgac ccggagaacc cgcgccagcc ggtgatcctg
     9601 gacgattttc cggccgccgc ggaaccgatc cacgccgcca tgccgcgtct gcgggcgctg
     9661 ctgtgcgcca gcgaggtgct gcgcaagcgg atcttccagg tggagttcct ggccaccctg
     9721 agcggcgagc tgatgatcag cctggtctat caccgccccc tggacgaggc ctggaccact
     9781 gccgcgcggg ccctggcggc cgagctgggc gcccagatca tcggccgcag ccgcggccag
     9841 aagatcgtgc tggaacggga ttggctgctg gaggacttcg aggtggacgg cctgcgtcgc
     9901 cgctaccagc agatcgaagg cagcttcacc cagcccaacg gcggcgtgaa ccgccagatg
     9961 ctggcctggg cgcgggcccg cgtggccggc gtggggggcg acctgctgga gttgtactgc
    10021 ggcaacggca acttcacgct ggcgctggcg ccctgcttcg agcgggtgct ggccaccgag
    10081 ctgagcaagt cctcggtcca tgcggcgcgc tacaacatcg ccctcaacga tgtcgccaat
    10141 gtgaccctgg tgcgtatgtc cagcgacgag atcagcgacg ccctggccgg cggacgggcc
    10201 tacacgcggc tgcgtgacgt ggacctggcg ggctaccgct tctcgaccct gttcgtcgat
    10261 ccgccccgca gcgggctgga tccggccacc gtcgcgctgg cgcggggctt cgggcgcatc
    10321 ctgtacatct cgtgcaaccc gcagaccctg cgcgacaacg tggcggccct gtccgccacc
    10381 catgccatcg aggccgccgc ggccttcgac cagtttccct acacccgtca cctggagtgc
    10441 ggggtgatgc tgcgtcggcg cgccgactga ccgccccttt tccggagaat cgccccatga
    10501 gcgccttgcc cccgtgtccc cagtgcgggt ccgaattcac ctatgaggac ggggcgatgc
    10561 tcgtctgtcc cgaatgcgcc cacgaatggt ccgccagtgc ggcccccgag ggcggcgaga
    10621 ctgcgcgggt ggtgaaggat gcccacggca ccgtgctgca ggacggggac tccgtcaccg
    10681 tcatcaagga cctgaaggtg aagggctcgt cttcggtggt gaaggtgggc accaaggtga
    10741 agaacatccg cctggtggac ggcgatcacg acatcgactg ccgcatcgac ggcttcggcg
    10801 cgatgcagct caagtcggag ttcgtgaaga aggcctgagt tggcaactga gtcggcaact
    10861 gaggcggccg cggagtcggc tggtctcagg cgcccgcggc ggcgtccgcg tccagcaggc
    10921 gctcgcattc cgccatcagg ctgcgggcga tgtccgagac gtagttcggg tcgagggctt
    10981 ccgccatctc gtgggcgatg tacagcgccg cctcccgcga cacactgtcg gccacctggc
    11041 gggcgaggcg gtcgctgaga gctgacaggt gttcccgttc ggcccgctcc agcgtgcggc
    11101 gcccgcggcg cagccagtcg gcggccaggg tggcgccctg ggcgtcggtg agccagcggc
    11161 cgtgttccag gaactggttc aggcgctccc cggcgagggc gaagatcagc gcgacgcgca
    11221 ggccgcggtc ggtgatggtg tccggcaaag gcaggtcgcg gggcatgggc ggcgtccaca
    11281 ggcatcggga ggcggacgct agcacgattg gggcaggctg gcacggggtt tgcaccggaa
    11341 gattccaggc acgatccggt gtcgcattcg atacgccggg cgatgccgcc gatttcgacc
    11401 gacccctgga gatcccgtca tgaacaccac ctgcgactgc cgcggcctgc ccgtggcccc
    11461 cggcgacacc gtccgcgtgc tggccgtcac cctcgacccg gacctggacg acgacgacgc
    11521 ggacatgttc cgcgacatga tcggcgccac ctgcgaggtg gagcgcatcg acggcgacgg
    11581 cgcggcctgg gtggcggtgt ggtggaacgg cttcgacggc ccgctgctga cgacggtggg
    11641 gctggatccg gcgcagatgg aaaagatggc ctgaggatgg cctgaggtcc gcgcgcgggc
    11701 ctgccgccgc gggcaggttc acgcccggcg cgacggggcg cgggagcggt ttgcagcgtc
    11761 cccgcgccgg ggccgccggc cctcactcgt cggcggcgga ggcgtcgtag ttcttcaggc
    11821 gcttctcggc cgacacgccg aggatcttca gcagccaggg cggcggcgat tcacgcatgg
    11881 cctgctggaa gcggaccagg acctcctcgg ccttgccgcc atccggtagc ttgatggggt
    11941 agatgtcggc gcggatcacc ttggcggcgg cgggaccgcc gatggacacc acgtaggcga
    12001 cctggcagtc gccgatcagc tgggcgcgcc acaggttctt gtccggcgcg aactctgcgt
    12061 ccatggtgtc gcggatgtcc accaggcgga tctcgtccgg cgacacctga tagaccagga
    12121 agcgcaggca ggagccgaag tggccggaca gggtgtcgcc ctggtcggag gcgatggcga
    12181 cccgcaccga gccaggcatg tcgccctcgg tgtagggctg gattcgcggc agggagtcgt
    12241 caccctgggt ctcgccccac aggatgcgca cggccagctt catggcctcc aggccgatcc
    12301 cgatgtcctc gccgtcctct tcgccgtcgg cgctggcgaa gccggtcttg agctgggtga
    12361 cggtgacctt gcgcaggtcg tcgagctgga tctcttcgcc gagctggcgt tgcaggagct
    12421 cgatcaggcg cggcagggtg gcgttgggca tggcccgggc cgcaagggcg atgcgcaggg
    12481 caacttcccg gctgatgggc gccgtggcag tttccatatt tttctcctct gtctgggctg
    12541 gggggtgccg ggacgacgca ggttcagtgc cagcggagaa agtgccgttt tttcaggggt
    12601 ttggaatgga ggcgccaacg tcctgggcgc ccgcgtgtgt cggatacccc acacggggtc
    12661 ggtcacgccg gtgccgggtc gccggcaaat accatgcggc cacgcccgcg gtccttggcg
    12721 acgtacatgg cgtcgtcggc ggccttcatg agggcgagat cgtcgacgcc ctggtccggg
    12781 tagagggcga tgccgatgga ggccgacacg gatagacggt gatcggcgat gtagaagggc
    12841 tcttccacgg tctgcaggat gcgctgggca acgatgcggg cgtcgtcggc ggcgtccacg
    12901 ggggccagca ggaccacgaa ctcgtcgcct ccgaggcgtg cgagggtgtc ggattcccgc
    12961 aggcacagct tcatgcggcg cgacacctcg cgcagcagat cgtcgccgac ggcgtggcca
    13021 taggcatcgt ttacgggttt gaagcggtcc agatccacga acatcagcgc cgccctggtg
    13081 gtgtcccgcc gggcccgcgc gaggacggac tggaggcggt cggagaaaag ggcgcggttg
    13141 ggcagcaggg tgagggggtc gtgctgggcc atcaggcgca tcacctcggc gttctcgcgc
    13201 agctcctggg tcatgctgcg ggcctgggca tgggcgcggg tctggcaggt gatgagctgc
    13261 cagatcaggg ccgcgatcag ggcgctcagg ctgatgccgg cgatgaggac gatctcggcg
    13321 gccttgctgc cgaagcggcg ttcgaattcg ggctggctgc gcaccaccag gcaccagtcg
    13381 tgaccggctg cggcgacgaa ctcgcgccct tcgaagcgga ccggcgccgc tgcgtcctgc
    13441 ccgccgcggc tgtcgtagag gagggcgtcg gggcggggct cgattccatc gtagagctgc
    13501 acgtcgagcc ccggcggcgt ctcgccggcg aggctggaca tcaggtcctt catgcggaag
    13561 gcggcctgga cccagccggt gagggcgtcg cggcgttcgg cctgggtgtt gagcggtcgg
    13621 tcgtggcgat acagggccag gtacaggata aaggcgctct ccggcccgtc ctgggtgtcg
    13681 gaatgcaggc gcaggcgctg gctgatggcc agggtgccgg aatcccgggc gtgaagcagg
    13741 gctgcccggc gggccgcgtc gatcatctgg tcatgcccga cggcgcgctg gttgccggtg
    13801 aggtccggcg ccgcatagat catcggggcg tgcaggccgt cggggccggg caccgcgcgg
    13861 ctgtaaacca ggctttgcag gccggcgaac tcgggcccct gagccaaggc atccacatag
    13921 gtggcgaaat cttcgcggtt gaccaggtcg gaggcccgga agaatccctg cacgccgcgc
    13981 agcatctggg tatagacggc gatgcgctgg tcgatgcggg cggcggtctg gcgcacgctg
    14041 gcgtcgaagt gggccgccat cgccgcctgg ttggcctgct gctcgtgctg ccacagcacc
    14101 agcgtgaggg ccagggcgcc gaagccggcg accaggggca ggaggagttc gcgcaggcgc
    14161 tgggcggcgg tcatctcaga aacgggtcat ggcacgggcc agatccggct tgaagtattt
    14221 gccgaagatg cgttcgacgc tgccgtcggc gcgcatgccg tccaccaggg cccgccagcg
    14281 ggcctgttcc tccggcgaca cggccgcgcg ggacatgatc aggccgtggg ggatgggcgg
    14341 atccgggaat tcgagggtgc gggtctgctc ggccaggcgc gcccgttcca ggatcgggat
    14401 gtcgaagggt tcgatgatca tggcctggat gtggtccgtc gccagggccc ggtacagggt
    14461 gtcgagggtg ttggcgtgga tgacccggcc ggtctgggcg agacgatcca ccagacggtt
    14521 ggcggtcggg ccgtagcgga aactgcggat cgcgcccagg cgcagcgcgg ggcgggcgtc
    14581 cagttcgtcg atgcgcgtca cgccggcgtc cttgcggacc agcagggtgt agcggttgga
    14641 gaaataccag gcgaaggcgg cgaagcgctc gcgggcgtca ttggtgatgc ccgacaggct
    14701 gaagtcgagg gcgccggtct cgatgagctg ccagatgcgg gcgcgcggga gcaccgtcac
    14761 attgaccggg cagccgctgc ggcggatcag ttcgtcggcg aagtccttgt cgatgccttc
    14821 tccgctggcg ccggaataga gcagaccatg ctcgtgcagc gccagggtgc gcggtcgcgt
    14881 gcagtccggc ccgtcggcgg cggccggacg ggtgaggcac agcatcaggc agacaaatgc
    14941 gcagtacaga agcatgaacg gacgggccgg ttcgggtgga ggggccggcg cgtactggaa
    15001 gcaacgggtg acccaagccg gcattgtccg gcagcgcggc gacggcgacg aaggactatt
    15061 catccgaaat tttgcgatat ttcacatttc gattcgatca ggccgacgcc gccgatccgc
    15121 ggcgccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gcggcttcct
    15241 cggcggcgcg ctcttcctgc tgcagggccc acatctgggc ataggcgccg ttgcgggcga
    15301 gcaggctggc gtggtcgccc cgttcgacga tgtggccctg gtcgagcacc aggatctcgt
    15361 cggcgttcat caccgtggac aggcgatggg cgatgaccag cgtggtgcgc ccccgcgcgg
    15421 ccgcgtcgag ctgggcctgg atggcctttt cggtcttgga gtcgagggcc gaggtggcct
    15481 cgtcgaagat caggatgtcc gggttcttga gcagggtgcg ggcaatggcg acgcgctgct
    15541 tctcgccgcc cgagagcttg agtccgcgct cgccgacgcg ggtctcccag ccctcgggca
    15601 ggcgcgcgat gaagtcctcc agctgggcgg cacgggcggc ggcttcgatc tccgcgcggg
    15661 gggcgtcggg gcggccgtac tggacgttgt agtagagggt gtcgttgaac agcacggtgt
    15721 cctggggaac gatgccgatg gcggcgcgca ggctgtcctg ggtgagggcg cggatgtcct
    15781 cgccgttgat gcgcacggcg ccgccctgca cgtcgtagaa gcggaacagc aggcgggcga
    15841 gggtggactt gccggagccg gaatggccca ccaccgccac cgtgtggccg gccgggatgg
    15901 cgaaatccac ctggtggagg atggcgcggg ccggctcgta ggcgaacgcc acatggtcga
    15961 agcgcacctc ggcggggccg cgggccagcg cgcgggcgtc ggtccggtcg gccacctcgc
    16021 ggcgggcatc gagcaggccg aacatgcgct cgatgtcggt gagggcctgg cggatctccc
    16081 ggtagagcac gccgaggaag ttgaggggga cgtagagctg gatcaggaag gcattcacca
    16141 gtaccaggtc gccgagggtc atctgtccgc ccgccacgcc cagggcggcg cgccacatca
    16201 tggccgtaac gcccaccgcg atgatcgcgg cctggcccat gttgaggagg gacagggact
    16261 gctggttgcg gatctgggcc tcggtccagc gcgccagctc ggcgtcgtag cggcgcgtct
    16321 cgaaggcttc gttgttgaag tacttgaccg tctcgaagtt gatcaggctg tcgatggcgc
    16381 gggcattggc ggcggaatcc agctcattga cccggcggcg cagggcggtg cgccagtggg
    16441 tgacgcgcac cgtgaagacg atgtagcagg tgagggtggc ggcggtgatg gcggcgaagc
    16501 tgggttcgta cttggtcagc aggatgccca tcacgaggcc gatctcaacc agcgtgggca
    16561 ggatggagta gagcgtgtag ctgatcagcg agccgatgga gcgggtgccg cgctcgatgt
    16621 cgcgggtcag gccgccggtc tggcgctcca ggtggaagcg cagggacagg gcgtgcaggt
    16681 gatggaagac ctgaagggag atctcccgca ccgcctcctg ggtgacccgc gcaaagatga
    16741 tctcgcgcag ttcggtgaac agggaggtgg agaagcgcag cacgccatag gccagcagca
    16801 gggcggcggg gaccacgatc agggcctggc cgcgggacag gtccaggtcg tccaccaggc
    16861 gcttgaagat cagcggcacc gacacattgg cgaacttggc ggagatcagg cagaccaggg
    16921 cgaacagcac gcggcccttg taggcccaca ggtagggcag cagctggcgg atcgtctgcc
    16981 agtcatggcg gtcgccggtg gcgggggcag gaggcgggcc ggagggggag cgtcgcacgg
    17041 aaggtcctcg ggacgaaggg tgtgaagtcg gggtgatggg cgctcaggcg cccgctgagg
    17101 gttcggccag cagacgcagg gcgatgccgg caaagatcag gccgcacagg cgatccagcc
    17161 acgggccgag ggccgggtgg cgggccaggg cgtgccccaa ggcaccggaa gcaagggcga
    17221 tgccaccgaa gaccacgacc gtctgcgcgc tgaacaccag gcccaggacg atcatctggt
    17281 gtgcggtgtc gccacgggcc gggtcgacga actggggcag gaaggccagg aagaacaggg
    17341 ccaccttggg attgagggcg ttggcgacga agccgcgccg cagactgcgt cgccatggca
    17401 ccgccgcagc gtcgcctgcc cgaacggggc ggctgccgcg cagggcctgg acggccagcc
    17461 ccagcagcca cgcggctccg gccacgcgaa gggcgaaaaa ggcccggggc gacgcggcca
    17521 gcagggcggc gacaccgagc accgtccacg tgaggtgggt cagacacccc agggcgcagc
    17581 cgagggcgat ggccagcccc gcacgccagc cctgggcaag ggcctggccg aggaccatca
    17641 ggttgtccgg tccgggcgcg agggtgaggg cgatcgagag gccgaggaag gcgagcaggg
    17701 tgtcgaagct gggcatgacc cgccgattct cggcggctgg cgcggaggcg tcaatggaac
    17761 gcccgtcgcg gcgacgggcg gttccccggc gctagtgggc gtgaccgtcg ccggcgtggc
    17821 acagcaccga cgggtcgccg acagccgcct cgcccatgcg ctgcaggtga tcgagcacgc
    17881 gcagggacgc ctgctccatg cggtcgagct ggcgcaccag gtcgaccgtg ttgccggcgg
    17941 tcagggcgcg caggacgtcg gcgccggccc ggtgcacctc gatgtggggc ggctcgactt
    18001 cccgatagcc cggaagccgg ctgaagcagg cctggccttc gcccgtgtag taccacttgc
    18061 ccaggcggca cgcggtgtgg tcgggcaggg cgccggcgtc caggggctgc aggccgaaca
    18121 ccgccaggta gatgcggaac ttgaagacga tgtggtcgat cttggccacc tccacgaagc
    18181 tcttcatggc gctgccggcg atgacgccct ccatgttgcc ggacagcgtc atcaggcgct
    18241 gcatgtcggc cgtggcgagc tgaccgcggg cgctgaactc accggccgcg cgggacaggc
    18301 tctccatggc gctgcgggcg cgggccgagt cctcgcggat gcggttcacc agggccgaga
    18361 tctcgttggt ggcggtgctg gtccgctccg ccagcttgcg cacctcgtcg gccaccaccg
    18421 caaagccgcg cccggcctcc ccggcgcggg ccgcctcgat ggcggcgttg agggccagca
    18481 ggttggtctg gtcggcgacg ccgtggatga gcttgacgat ggtgccgatt tcgtcggcgc
    18541 gctgggcgag ggtgtccacc tcccgcgccg actgggacga ttcgtccgcc aggcgttgca
    18601 gattggcggc gatctcggtg gtggcctgac cgctcgccag ggacacatgg gccgcctcca
    18661 cggccttctc cttctcgtcg cgcagcagcc gcgccatctc gcccaggctg ccctgggcgt
    18721 ccgtcaggga ggtgccgaac tccgccatgc gcgtgatgac ggcgcgcagc atggcggctt
    18781 cattgtcctg tggggaaggg gccgcacggg cggcaagctg ggcgcgcagg gcgtcgatct
    18841 cggcgttcag cgcgctttcc tgacgctgtg ccccgtcgag ccggcgctgc atgtcttcga
    18901 atcggtgtcg ggcgatccac atagctgttt ttcctggtgt atcggcattc cttcgggatg
    18961 ccggcgagcg atgtcctctc gggctggatg tcggttccgc cgcgcatttc ttgaggtcag
    19021 ggacataaat tgtttccgtt ggccacggca ggcgcgacaa tcgaccccgg accgatccgg
    19081 tccgccgcgg aggaatttca tcatgaattg tcgcgacgaa cgcctgcccg ccgcgctgct
    19141 gggcctggtc gcgctggtcc tggcggtgtc cgccatcgcc ccctacgacc gggccacctg
    19201 gtggatggag gtggcgccgg tgttcctggt ggcgccgctg ctgatcgcca gccaccggcg
    19261 tttcccgctg acgccgctgc tccagacgct gatcgcctta cacgccctgg tgctcatcgt
    19321 gggcggcgta tggacctacg cgcgcgtccc cttcggcttc tggctgcagg atgtctttca
    19381 cctggcgcgc aatccctatg accgcatcgg ccacttcatg cagggcttcg tgccggccct
    19441 ggcggcgcgg gaggtcctgc tgcggcgcgg gatcgtgcgc ggacgcggct ggctgttctt
    19501 cctggtgacc tgcgtgtgtc tggccgtctc ggccctctac gaactcatcg aatggggcgc
    19561 cgccgtggcc ctcggccagg gggcggacgc cttcctcggc acccagggcg atccctggga
    19621 cacccagaac gacatggcca tggccctcgt cggcgccctg gtggcgcaga tcggtctggg
    19681 tggctggcac gcgcgccaga tcgcgcgcct gcgcctgtag caaagccgac aggcgcctgc
    19741 acacgccgcc ccgatcgccg gactggcgcc cgcggcggcg cgggatcgac gcttggtacc
    19801 gatcttgctg agtcgggttc gacctcaaaa tattgtcgac ctcaagccat gcccgacctg
    19861 cccgtcatgc cgccccttga agccgctccc gccgagcccc ccgcggcgcc gatcacgccc
    19921 gccgacctgg tgctggccta tcaccggcgc acccgtcacc gcttcgacgc ttacgcccgc
    19981 ggcccctcca cactggattg ggacgcccag ccggcgccct tccggcattt cggcggcacg
    20041 cgccgcatcc gcctgcccct ggcctgtgcc gaggcctgtg ccgcgccgcc cgccacgccg
    20101 gccgcggcgg atctgacggc cctcggcacg ctgctgcacc tgtccttcgg catcaccgcg
    20161 tggaagcgcc tggggccgga ccgctgggcg gtgcgcgcca acccgtccag cggcaacctg
    20221 catccggtcg aagcctacgt gctggtggcc ggcctgcccg acctggagga cggcctctac
    20281 cactacctgc cggatgtcca cgccctcgag ctgcgggccg cccatccgcg ccccttcgcg
    20341 gccgggccgg cattggccgt ggggctgacc agcgtcatgt ggcgggagat gtggaagtac
    20401 ggcgagcggg ctttccgcta ctgccagctc gacaccggtc acgccctggc ctgcctgcac
    20461 tacgcggcgg ccctgctggg ctggcgggtg agcgaccggc ccggcctggc gtcggccgcc
    20521 cttgcccgtg tgctgggcac cgaccgggac gaggacttcc ccggccgtcg ccctgacgtg
    20581 agccgcgagg aggtcgagct gctggcctgc gtcggtccgg cggacggcct gccggtcgtc
    20641 accgccgacg ccctcgccga ggccgcggac gccgcccgct ggcagggcgt cgccagcgag
    20701 gtggaccgtc accccatgtt ccgctggccg gtggtggacg aagtggccgc cgccagccgg
    20761 cgcgtgtccg gtgtcccggt gacgccgcta ccggtggcga tcccgcggcc ggcatcggcg
    20821 cggcacatcc tgcagcggcg cagcgcccag cgtttcgacc cggcccacgt gatgccccgc
    20881 gaggacttcg tgcgcctcct cgacgccctg cgtccggtgg cgggcgggcg tctcggcctg
    20941 gccctgttca tccaccgggt ggacggcctc gcgcccggcg tctatctgct ggcccgcgcc
    21001 gactggctgc ggccccagcc gacccgcgcc ggggcgtccg cgctgcagcc cgtgaccggc
    21061 tgtccggacc tgtactgcct gtccacctcg gaatccgtcc ccctgcgccg tctggcgcgc
    21121 agcctgcact gccaccagga catcgccgcc aacgcctgca tcgccttcgg tctgctggcg
    21181 cagctggagc gggaggtcgc cgacgatccg gcccgctatc gcgacctgct gcgggaagcc
    21241 ggcttcctcg gccaggtgct ctatctggag gccgaggcca tgggcctgcg cggcaccggc
    21301 gtcggctgct atttcgacga cccggtgcac gaactggtca tcgccggcca gcgggatgcc
    21361 cactccctct accacttcac catcggcctg ccggtggacg acggccgcat cgagaccggc
    21421 cccgcctacc ccgccacgtt ggagatgccg tcatgaacgc ttccgccacc gcgcccctgt
    21481 ccgccaagtt ccagcgcatc tccgccgccg aggccgccgc gctgctgcgc cgtccggaag
    21541 gggcgccgcg tcccgtgctc ttcgacgtgc gcgaccgggc cagctacgag cgtgcccacg
    21601 tgagcggcgc cgagcatctc accgacgccc atctgggcgc cgccctgcgc cgcgtgccga
    21661 aaagcgcggc ggtgatgatc tactgctacc acggcaatgc cagccaggtg tatgccgaga
    21721 ccttcgccga tttccgcttc gccgaggtgt acagcgtcga tggtggctac gagcccctcg
    21781 ccgcggcact ggccctcctc gacggcgtgc gcttggcgcc gcgtctgccg gacggggcga
    21841 gcgccgacct gcgcgccttc gtcctccgcc acgccttcga tccggccgat ctggacgcgc
    21901 cgcgggcgca gggcctcacc ccgctgatgc gcgccgccct gcaggggcag gccgaactgg
    21961 tgggggaact gctcgccgcg ggcgtggagg tggatcggcg caacggcgac ggcaacaacg
    22021 ccctgtggct ggcctgcgtg tcgcgggatg ccgccaccgt ggcggccctg gtggccgccg
    22081 gcatcgacct ggacaaccgc aacgacatgg gcgccacctg cctcatgtac gccgcctcca
    22141 gcggtcgccc caccatggtg gaggccttgc tgaaggctgg cgccgatccc tacgtgcgca
    22201 acttcgacga cgcccgcgcg gtggacctgt gcgccaccct cgagtgtctg cgtctgctgc
    22261 gtcacacggg caactgagcg ccacgcggcg ccccccgacc ccggagagag atcatgagca
    22321 aataccgttc gattgccctg ctgatggaag ccgtcgaggc gcccctggcc gcggccggcg
    22381 tcgtggccgg cgaggcctgg cgtgccgacg aacaggcggt gggcttcttc agtcacgccg
    22441 acgtgggcct ggcggcgtat gtattcacct acggccagcg ttccgggcgc tatgggatca
    22501 acctggactt cccgctgccg gcagaaggcg gtccgctcct caccgtgccc accagcgccg
    22561 aggatctgcc cctcgcggcg gtcatcgccc tgctgctgga gcacttcgac gtgccggtgc
    22621 tggaagcggc ctgagcccca gccggccgga cccggccggc tcccgaacgc gatcaatggc
    22681 gtccggctgt gcgtgccgca cgctgagcgg gcggacactc cggccgctcc ggcacgacgc
    22741 ccgctgtcat gatgaggacg gcgatccgca tgtgaggcgc gtcccccggt caccgccgct
    22801 ggcgcgccgt accgaccgcg gaccgggccg cccgcggcgc ctcagtgcag cgtgcgcggc
    22861 atcgccagcg tgaattccgg gatcgtcgcg ttgaaatcga ccccgtcctc cgtctgcatc
    22921 cggtagctgc ccttcatcgt gcccaccggc gtggtgagca cgcagccgct ggtgtactcg
    22981 aacgcctccc ccggcttgag ggtcggctgc tggccgatca ccccgctgcc ttccacctcc
    23041 tggatcgtgc cgtgggcatc ggtgatgatc cagtgccggc tgaggagctg ggcgggcgcg
    23101 tcgcccgtat tgcggatcgt gatgtgatag gcgaacacgt agcggtccgc cgcctcgtcg
    23161 gactggtcgg ccagatagcg ggcgaccgcc ttgacttgga tggaatccgg atcggggagc
    23221 gtggtcatcg tcggcgcatc ggttgcgagg atgacgcatt gaagcccatc gccctcatcg
    23281 ctgcaagtcc ggctttcccc aacgtggcga acggtccgac gggcgggtac actgcgccgt
    23341 tttccgcgtc tgccaggatc gaccatgagc ttccgcatcg cccccagcct gttgtccgcc
    23401 aacttcgccc agcttggcgc ggaagtcgcc aacgtgatcg ccgccggtgc cgactggatc
    23461 cacttcgacg tgatggacaa ccactacgtc cccaatctca ccatcggccc cctggtctgc
    23521 gaagccatcc gcccgtgcac caccgcgccc atcgacgtgc atctgatggt caagccggtg
    23581 gatcgcatcg tgccggactt cgccaaggcc ggcgccgaca tcatcacctt ccacccggag
    23641 gcctccgagc acatcgaccg tacgctgggc ctgatccgcg acagcggctg ccaggccggc
    23701 ctggtcttca acccggccac gccgctgcac tggatggatc acgtcatgga caagctcgac
    23761 gtggtgctgc tgatgagcgt caacccgggc ttcggcggcc agtccttcat ccccggcacc
    23821 ctcgacaagc tgcgcgaggc gcgggcgaag atcgacgcct acacggcccg cagcggccgc
    23881 cccatcctgc tggagatcga cggcggcgtg aaggtggaca acatcgccga gatcgcccgc
    23941 gccggggcgg acaccttcgt ggccggctcc gcggtcttcg gcgccggccg cgacaccgac
    24001 ccgcaccgct acgacagcgt gatcggcgcc ctgcgcagcg ccctggcggg ggtctgaggt
    24061 gtccccgcgc cacgcggtgc tgttcgatct ggacggcacc ctgctcgatt ccatccccga
    24121 cctggccgaa gcctgccagc gcatgatgcg ggagctgggc catccgcccc acgacctgga
    24181 gacgatccgc agcttcgtcg gcaagggcat ggtcaacctc gtcacccgct gtctcggcga
    24241 cgcctccgcg gaagcggtgg acgccgccgt ggcggtcttc cgccgccact acgccgcggt
    24301 caatggcgac gccaccaccc tctatccggg ggtggtgccg gccctggagg ccctcgccgc
    24361 gcggggcgtg cccctcgcct gcgtcaccaa caagccggcc gccttcaccg agcccctgct
    24421 ggcgcgctgc ggcattgccg attactttgc cgtcaccgtc agtggcgaca ccctcgccga
    24481 gaagaagccc catcccgccc ccttgcagca cgcctgtcgc ctgctcggcg tggcccccgc
    24541 ggcggccctg atggtgggcg actccgccaa cgacgccgaa gctgcccgcg ctgccggcat
    24601 gccggtgctc ctcgtcacct acggctacag tgagggacgc ccggtggaca gcatcgactg
    24661 cgacgggcta ctatccagcc tcgaggacgt gctgaggcac ctccccgcct gaaatccccc
    24721 ggaatttcat ccccttcatg acccgtaagc gcatgttctt cgtcgaggag ttccctgccg
    24781 tccgtatccc ccgctccggc ggatgcggcg gccggtcatg gctgcgcgcc tgacgccgcc
    24841 ctcccgtcgc gggccaagcc ccgtgatacc ctcgcgaaca cgcagcatgc ccttccggag
    24901 tctccatgac cgaagccgaa ttcaacgccc tggccgccca gggctataac cgcatcccgg
    24961 taaccgtcga caccttcgcg gatctcgaca cccccctctc cgtctacctc aagctcgcca
    25021 acgcgcccaa cacctacctg ctcgaatccg tgcagggggg cgagcgtttc gggcgctatt
    25081 ccatcatcgg cctggcctgc tccacgcgca tcgaggcgcg cggccgcagt gtgatgcttc
    25141 tcaccaacga ccgcgtcgtg gagcgtcgcg acgacaccaa tcccctcgat tacgtggccg
    25201 aattcaaggc ccgcatcaag gtgcccccgc gcgaccgcct gccgcgcttc gccggtggcc
    25261 tggtcggctg cttcggttat gacacggtgc gctacatcga gccgcgcctg gccagcaccc
    25321 acaagcccga cgccctcggc acgcccgacg tgctgctgct cctgtccgaa gagatcgcca
    25381 tcgtggacaa cctgtccggc aagctcaccc tcgtggtgta tgccgagccc caggtgcccg
    25441 atgcctacgc ccgcgccacc cggcggctca aggaactcat cgggcgcctg cgcgagcccg
    25501 tgtcgattcc cgccgaggcg cccggcgccc ccgccgaggc cgtctccagc ttcggcgagg
    25561 cggccttcaa ggacgccgtg cgcaaggcca agcactacat cgtcgagggc gacatcatgc
    25621 aggtggtgct cgcccagcgc atgagcaagc ccttcgccgc cagtccgctg gccctgtacc
    25681 gggcgatccg cagcctcaac ccgtcgccct acatgttcta cttcaacttc gaggacttcc
    25741 aggtcgtcgg cgcctcgccc gagatcctcg tgcggctgga ggacaacgtg gtcaccgtgc
    25801 ggcccatcgc cggcacccgc aagcgcggcg ccacccacga cgaagacctg gccctggagg
    25861 ccgaactcct cgccgacgag aaggagcggg ccgagcacct gcagctcctc gacctgggcc
    25921 gcaacgactg cggccgcgtg gccggcaccg gctccgtgcg cgtcaccgag cagttcaccg
    25981 tcgagcgcta ctcccacgtg atgcacatcg tctccaacgt ggaaggccgc ctgcgcgacg
    26041 ggctcgacgc cctcgccgtg ctgcgcgcca ccttcccggc cggcaccgtc tcgggtgcgc
    26101 ccaagctgcg ggcgatggag atcatcgacg agctggaacc caccaagcgc ggcatctacg
    26161 ccggttcggt gggctatgtg ggcttccacg gcgacatgga cctggccatc gccatccgca
    26221 ccgcggtgct caaggacggc cacatccatg tccaggcggg tgccggcatc gtcgccgact
    26281 ccaaccccga cgccgaatgg gaagagaccc agaacaaggc ccgcgccatg ctgcgcgccg
    26341 ccgagatcgc cgaagccggc ctcgacacgc cgctgtgaac gcccggcaaa aggccgcccg
    26401 caggcggcct gaagcgctgc accggcacgt ccggaccgcg cccgcccatg tcggtggggc
    26461 ggcggtccgg aggcgccgcg cgtcagaagg cgatgcccac cttggcctgc accagccagt
    26521 tctcggcgcc ctgcatgccg ggggcgatgt ccttgttcac cgtgtcctgc cagcgcgcgc
    26581 cgaggcccag attcacctgg ccgatgcggt aatccaggtc tgcgccggcc tgccaggcga
    26641 acaggtcggt ggtgcgaccg cccgcgtccg ccttcacgta gccgatgccg gggccgacgc
    26701 cgaaggtcag gttctgggcc accgggatcg tccagcgcgg gttcagttcc acggtgttca
    26761 gggtcacgcc gtccttgtca tagcggttcc acgacagctt ggtgcggacc acgccggtgg
    26821 gcgtctggaa caggccgcag ttcatggcca gctccaggcc cagggcggtg tcgctgctgc
    26881 cgtgcttggg gtcgagcatg ccgccggtca ccgacgcgac gaagttgggc cggtagtcct
    26941 tgtccaggat gggcaggaac ttccagtcac cggcaaaggc cggggcggtg gcggccgcca
    27001 gggtgacggc ggtcagcagg atgcgggcgt tcttcatggt tcgtcctctt ggtgttgtgg
    27061 aaaatggatc catcatgacc gatggcgccg ctcgaagtcg gtaaccccgg tcacatagcc
    27121 atttttccgt cccgcgcccc ctgcgacggg tcggaactgc gagaaaatgt ttcagcgtcg
    27181 cgcccccgcg ggtcgcggcg attgccgaaa ggaagtccag ccatgctgct gatgatcgac
    27241 aactacgaca gcttcactta caacctcgtg cagtacttcg gcgagctcgg tgccgacgtg
    27301 cgcgtgttcc gcaacgacga gatcaccgtc gagcagatcg ccgaactggc gccggcccag
    27361 atcgtcgtct cccccggccc ctgttcgccg gccgaggcgg gcatctccgt cgccgccatc
    27421 cagaccttcg ccggcaagat cccgctcctc ggcgtgtgcc tcggccacca gagcatcggc
    27481 gccgccttcg gcggacgcat cgtgcacgcc aagcagctca tgcacggcaa ggtgtcgccg
    27541 gtccatcacc tggaccaggg cgtcttcaag ggcctgccca atcccctcac ctgcacccgt
    27601 taccactccc tcgccatcga acgcgagacc ctgcccgact gcctcgaagt caccgcgtgg
    27661 accgacgacg gcgagatcat gggcgtgcgc cacaagaccc tcgcggtgga aggcgtgcag
    27721 ttccaccccg aatccatcct caccgagcgc ggccacgaca tgctgcgcaa cttcctcgac
    27781 gcatcccgct gaacggagcc cgccatgaac ttcacgcccc aggaagccct gcaacggacc
    27841 atcgaacacc gggagatctt ctacgacgag atgctctccc tgatgcgcca gatcatggcg
    27901 ggcgaggtct cgccggtgat gaccgccgcc atcctcaccg gcctgcgggt caagaaggag
    27961 accatcggcg agatcgccgc cgccgccacg gtgatgcgcg agctctcctc caaggtcatc
    28021 gtgccgccgc cccacgagca cttcctggac gtggtcggca ccggcggtga cggcgcccac
    28081 accttcaaca tctccaccgc caccatcttt gtcgccgcgg cggccggcgc gcgggtggcc
    28141 aagcacggcg ggcgcagcgt ctccagcaag tccggcagcg ccgacgtgct cgaagccctc
    28201 ggcgtcaacc tcaacctcaa cgccgaacag gtcgccgaat gcatcgcccg caccggcatc
    28261 ggcttcatgt tcgcccccaa ccaccacagc gccatgaaga acgtggcgcc ggtgcgccgc
    28321 gagatgggcg tgcgcaccct cttcaacatc ctcggccccc tcaccaaccc ggccggcgcg
    28381 ccgaacaccc tgctgggcgt cttccacgcc gacctggtgg gcatctgcgt gcgcgtcatg
    28441 cagcgcctcg gcgcccatca cgtgctggtg gtgcatgggc gggacggcat ggacgaagtc
    28501 tccctcggcg ccgccaccct ggtgggcgag ctcaaggacg gcgagatccg cgaatacgaa
    28561 atccacccgg aggacttcgg cctcgccatg atgtccagcc gccgcctgca ggtgagcacc
    28621 gccgacgaat ccagggccat gctcctgtcc gtcctcgaca acgtcccgtc cgccgcccgt
    28681 gagatcgtca tcctcaacgc cggcgtcgcc ctctacaccg ccaacctggc cgactccatc
    28741 ggcgccggca tcgccctcgc ccgcgaggcc atcgcctccg gcgcggcccg ggccaggctc
    28801 gacgcctttg tcgccgccac ccgttccttc gcctgagcgt cccatgtccg acatcctcaa
    28861 caagatcctc gccgtcaaac gcgacgaagt cgccgccgcc cgcgcccgcg tctccgatgc
    28921 cgccgtgcgc cagctcgccg ccgaccagcc cgccccgcgg gatttcgtcg ggtccatgcg
    28981 tgcccgcctg ggcgccggcc gttccgccgt gatcgccgag atcaagaagg ccagccccag
    29041 caagggcgtc atccgcgccg acttccgccc cgccgagatc gccccgtcct acgagcgcgc
    29101 cggcgccgcc tgcctctccg tgctcaccga ccgccagttt ttccagggcg cgccggacta
    29161 cctccaggcc gcccgcgccg cctgcaccct gccggtgctg cgcaaggact tcctcgtcga
    29221 tccctaccag gtcttcgagg cccgcgcgat gggcgccgac gccatcctcc tcatcgtctc
    29281 cgccctcagc ctcgccgaga tgcaggccat ggaggccatc gccgaatccc tcggcatggc
    29341 cgtgctggtg gaagtccacg acggcgccga gctcgacctg gccctgcagc tgcgcacccc
    29401 cctggtgggc atcaacaacc gcaacctgcg caccttcgaa gtcagcctgc agaccaccct
    29461 cgacctgctc ccgcgcatcc ccgccgagcg catcgtcgtc accgaatccg gcatcctgca
    29521 gcccgccgac gtggccctga tgcgcgagca ccgggtcaat gccttcctcg tcggcgaagc
    29581 cttcatgcgc gccgacgatc cgggcgccgg tctcgccgcc ctgttcgcct gatccgccat
    29641 gggcgacgcc gcgccgccgc ctgaccccgc caccccgccg cccgcgcagg ccc
//
LOCUS       DAOQPA010000033        29158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_722_length_29158_cov_18.269972, whole genome shotgun sequence.
ACCESSION   DAOQPA010000033 DAOQPA010000000
VERSION     DAOQPA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29158
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_722_length_29158_cov_18.269972"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="PKX01_06675"
     CDS             complement(<1..1473)
                     /locus_tag="PKX01_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75846.1"
                     /translation="MSFTRLRLDTPYPGIRPFREDEEHLFFGREAQVDALVDTLARGR
                     FLAVIGSSGCGKSSLVNCGLRPALHRGLLAQAGSAWRMATCRPGSQPLRALAESLAAP
                     GALHRDLPDSPFRLAELIESTLAMSRRGLVEAFLQAGLPAGTQLLVIVDQFEELFRYR
                     GLGPRGDAQPDADGDPATAFVNLLLEARAQDVPIHVVLTMRSDFLGDCARFAGLPEAI
                     NQGQYLVPRMSRDERRAAIAGPARVCGADIAPTLLTRLVNDVGDDPDQLSLLQHALAR
                     TWAHWRASRTPSPIEARHYDAVGTLAHALNQHAEELYAALPPGRQRLCAQAFRALTDT
                     GSDHRGVRRPCRFADLVALGGAPAEELATTLAPFRAPGAAFLMPPEGVALTADTVVDL
                     AHESLMRIWDRLRAWGEREAEAARMLRRLADAADLHARGEASLWRDPELQFALDWRQH
                     NTPNPAWAALYGRALAPALAFLDASRDARDAAAAAEIARAE"
     gene            complement(1470..2957)
                     /locus_tag="PKX01_06680"
     CDS             complement(1470..2957)
                     /locus_tag="PKX01_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HMZ75847.1"
                     /translation="MKYARQIFISYAHLDNQPLTADQAGWVSLFHATLQTLLSQRLGA
                     PADIWRDDKLRGNDVFSDEILGQLAGTALFVSILTPRYLKSEWCAREIRSFCERAEAE
                     HSLFVDNKSRLIKVLKLPVDGDGLLPPAAAQLLGYEFYEFDEDRTPRELDPAYGEQPR
                     QAFLRKANKLAWDLAAQINALADTPAANDGNATPPAAERPAVFLAECSRDRREARERI
                     EAELACCGYTVLPDQRLPDDEDEHVAAVDALLARCVLSIHLIGSGSGRIPDGARQQSV
                     VVLQNERAAARSRSAGLRRVIGLPPGLASPLPAQQAFLDALRQDAALQAGADLVEGDL
                     EVLKAAIHAALKPPEAPPAAPSVSARRVHLVCDARDRKDILPFIRLLRAHGVEVTLPV
                     FSGDAGAVREANRQLALDCDVLVLWYGAGDEAWKYHQGNDLQKLCASQRERPLRGQFV
                     LLAPPLGDDKAFLRALAEADTLDALDGLDAAVLAPLLAALGTEAP"
     gene            complement(2954..3793)
                     /locus_tag="PKX01_06685"
     CDS             complement(2954..3793)
                     /locus_tag="PKX01_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HMZ75848.1"
                     /translation="MRLLVCTKRDLHGARLLNRLLPSLTGHELVGVWLSDKQRPAEAE
                     LPELAELACLERHLPVDLLFPLVDALPPALADTAPAASFHGLARRHGVNLSVFDELPD
                     AATRLAALAPDLVIVARFSHLFDAAALAVPRFGLLNLHPGHLPRYAGLHAPLRSVLEG
                     ETQLACSVHWITAGIDDGPLVAVHRTPFDPAGGLYTQIGELYALALPTVQAVIAACAA
                     GERPPGTPQDRRLRRYRSLPGPDDFRAFAARGLRLWQADAYRAQLAAFLPPGLALPGG
                     LPR"
     gene            complement(3796..4830)
                     /locus_tag="PKX01_06690"
     CDS             complement(3796..4830)
                     /locus_tag="PKX01_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ75849.1"
                     /translation="MKRLAYLAPALAALAVLLTAQGAAHAAPAEHPPYRIAMLLYRGC
                     EEACRGFQAYFRQHGIAAEFTLRDAAQDRRRIAGFVQELRREKPDLVVTWGTTVTEEA
                     AGRWNAVDPRRHLTDVPVVFMIVSDPVAAGIVQRLDKPRRTLTGTLYLLPVETQIKAA
                     HSYLAFRTLGYILNETESNSITTRDALRALAPSQGFTLVERALPVSAAGTPDATALPA
                     LLDALKGAGVDLLYQGPDSFLNSRRDVLTDGALARGMPVFAAAEAPVQNGRALFGVVN
                     KYEDVGRFTAAQALRILRDKVSPAAIPVELPRNFSYLINLPVALQLGRYPPLKLLDVA
                     EIVGLDGADR"
     gene            complement(4827..7139)
                     /locus_tag="PKX01_06695"
     CDS             complement(4827..7139)
                     /locus_tag="PKX01_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ75850.1"
                     /translation="MPPRAEPPSLPAAPPPDLRQWLRIGLLLLAALGLALSLQAVLLF
                     HKERDLLTGITVSRIGLLAEQIHSSFQRASANGLQLGEFRALAARLPALAASAQDVTA
                     IQVLDEDGRRLFATGPTDLPVPSPPRAARGLWHATAPLGYMWIGRTLQDSTGESVGSV
                     LIAVRSAALDARIEAARAVAGPRLALTLGLVAVALPVLLVLAMRLPGPFSLRRRVLGA
                     ALCLATAGSLHLSLAALPSFSERLAPALDDKAHALACFLSTRIDAALALGIPFERLNG
                     VEAYFTDALAQHPEVLALRLQGPTHRYAASRPGEAGHALEVPISAGVRATGATLVAVT
                     DAGVVARELRALAVDLAIVFLVALVLFNEALCAILGSARFAAGPRERLGPARLAVFLL
                     ILAEELTRAFLPVHIRALVAQTAAEDALTGPLVGLPISAYMASFALLTPFAGHWAVRF
                     GVARTFALGAGLSAAGFLGAMAGGGYGAFVAARCLCAAGYAIGTLAIQQHFLSTATAS
                     ERTRALALFVGAVQTAAICGSPVGGLLAERFGATAVFAAAAAMSGLAFLVQRLAVPVA
                     PPAAGAPAPRLLPLLTRGRVVIPLLTAALPAKVVLAGFLFYLVPLALAGADYSPASTG
                     RAMMLYFVLVAAANPLASWLSDRFGWQRSLVIAGGLVIGAGGVLGLLDRIETLFAGIV
                     ALGLGTGLSAAALQALASRDGPAAIVLLRTVERLGAVIGPLFVGSLLAVTAWGGAMAT
                     VGATMLVATLAFALWARAAASLDTPDKEPS"
     gene            complement(7295..8335)
                     /gene="ftsY"
                     /locus_tag="PKX01_06700"
     CDS             complement(7295..8335)
                     /gene="ftsY"
                     /locus_tag="PKX01_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMZ75851.1"
                     /translation="MFGFLKKTPKADDAPASPDAPVATPASPAAAPEKKSWMDRLRAG
                     LSRTRQQIGGGLSSLFSRRKIDEELLEELESTLLMADCGVEATQYLIDELRRRWKRDK
                     LETADQLQGALAEGLLSILAPLEKPLEISGHSPYIIMIAGVNGSGKTTSIGKLAKHFQ
                     NQGKSVLLAAGDTFRAAAREQLMTWGERNGVAVIAQESGDPAAVVFDAINAARARGID
                     IVLADTAGRLPTQLHLMEEIAKVRRVIQKAEPSGPHEVLLVLDANIGQNALQQVKAFD
                     AAIGVTGLVVTKLDGTAKGGVVAAIGRQCPKPLRFIGVGEQIDDLQTFRAREFVDALF
                     APAGGAPADPPA"
     gene            8496..9884
                     /locus_tag="PKX01_06705"
     CDS             8496..9884
                     /locus_tag="PKX01_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ75852.1"
                     /translation="MNPRGRLASTLAALCAVAGLAVVAPARANPFETVLPNGMKLVVK
                     EDRRAPSVVHMVWYRVGAMDEVDGTSGVAHVLEHMMFKGTKAVGPGEFNKRVAAAGGR
                     DNAFTSYDYTAYFQQVPKDALPGMMALEADRMAHLRITDAEFRKELEVVKEERRLRTD
                     DKPRALVIEQLMASAFQAHPYRRPIIGWMNDLDAMTAQDARDWYVRWYVPNNATLVVV
                     GDVDHAAVFREAAATYGRVSARPLPERKPLREPEQSGPRSTVVKAPAELPYVALAWHV
                     PTLRDVKADGDYYALQVLAAVLDGYEGARLAKNVVRGSRVAVSAGAGYDGTARGESLF
                     VLDGAPAPGKTVADVEAALRGELARIQNEGVSEDELRRVKVQAVAAQVYKRDSLMGQA
                     MEIGMDEMVGLSWKDDDYQLARLRAVTAAEVQAVARKYFSDDGVTVARLDPLPVDPEA
                     RARAAAAAPHRH"
     gene            9907..11253
                     /locus_tag="PKX01_06710"
     CDS             9907..11253
                     /locus_tag="PKX01_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ75853.1"
                     /translation="MMISVSRFGAALALTLFGLCGGAHAGPAIQTWSTPNGARVFYVE
                     SHALPLVDVQVDFRAGGAESPAGKTGVAGLTHALLDNGAGDLDEEAIANRIADLGAQM
                     GGSADMDRASITLRTLSSAAERDGAVDLLATVIQHPTFPAASFEREKARTLAGLKESD
                     TQPDAILSRRFAAAVFPGHPYGRLPTVDSVAALNRDDLAAFHARTYTAARAVVSIVGD
                     VSRADAEAMAAKLTADLPRGEAPATSVNTVLPEAQTIRVAHPSAQAHIAIGQPGIRRG
                     DPDFFPLLVGNYVLGGGGFVSRLTKEVREKRGYAYSVFSYFMPQRDVGPFEIGLQTKG
                     SQTDDALQVVNATLAEFLDKGPTGAELQAAKDNLVNGFALRLDSNRKVLEQVAVIGGF
                     GLPLDYLDRFPDKVRAVTAAQVRDAFRRHVSPAHLVTVVVGGDGDKAAAPRAEVAR"
     gene            11250..11786
                     /gene="rsmD"
                     /locus_tag="PKX01_06715"
     CDS             11250..11786
                     /gene="rsmD"
                     /locus_tag="PKX01_06715"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HMZ75854.1"
                     /translation="MSRIRIIGGEWRSRLIDVSTVKGLRPTPDRVRETLFNWLGQDLD
                     GRTCLDLFAGSGALGFEAASRGAAAVTMVEQDPVAFAALQKNTKTLLASRVELVRGDA
                     LKFAGSASRRFDVVFFDPPYRAGWIDRMAPFLPDLLTEHGVIYVEAEYKIENFDRLTR
                     IKHGVAGQVYYHLFAAQA"
     gene            11820..12311
                     /gene="coaD"
                     /locus_tag="PKX01_06720"
     CDS             11820..12311
                     /gene="coaD"
                     /locus_tag="PKX01_06720"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMZ75855.1"
                     /translation="MRDSVAVYPGTFDPFTRGHEDLVRRAARLFDRVIVGVAASGSKG
                     PIFSMEERVEIAREVLTAYPNVEVQGFSCLLMEFLRRNEARVILRGLRAVSDFEYEFQ
                     MAGMNRKLFADVETVFLTPADEYMFISATMVREIARLGGDVSKFVQPGVNARLVQKIN
                     YSK"
     gene            12328..12579
                     /locus_tag="PKX01_06725"
     CDS             12328..12579
                     /locus_tag="PKX01_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HMZ75856.1"
                     /translation="MALMITDECINCDVCEPECPNGAISQGEEIYQIDPNKCTECVGH
                     FDEPQCQQVCPVDCIPFNPEFPETKDDLMEKFLKLTADK"
     gene            complement(12660..14612)
                     /locus_tag="PKX01_06730"
     CDS             complement(12660..14612)
                     /locus_tag="PKX01_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HMZ75857.1"
                     /translation="MLAEHFSAYTQWRGEVASGIADLRKWLQQNEVGDAQSDLRLQYL
                     LDRLREDKLIVAFVAEFSRGKSELINAVFFAGYGNRILPSSAGRTTMCPTELQWEDGD
                     TPCIRLLPIDSRRSNASISELKRFPDEWRVRPLDTSSAESLQAALAQVGETRIASREE
                     AEALGFPIDESGEKGIRPDTGGTVEIPAWRHAVIQFPHPLLEQGLVILDTPGLNAIGA
                     EPELTLNLLPNAHAVLFILAADTGVTQSDLAVWREHVNAGRRQRGRLIALNKIDGLWD
                     GLRSEADIEREIAGQVASVAATLDVAPEQIYPVSAQKGLVAKVNNDDDLLTRSRLPEL
                     EYALSTELLPTKRDIVRDNTYGEVQDIIARCRELLGARLSGLREQLTELTDLRGKNQN
                     VIEYMMRKVRSEKDEFEQGLQKYYAVRSVFTNLSNNLFGHLGLDALRDETRRTREAML
                     DAAFSRGLRGAMKGFFQHLRNNLKKSTDEIAEISKMLDAMYKRFTVEHGLKLAAPSSF
                     STLRYEKEIERLEDAFNSQFDTVLNIVTTEKHTLTQKFFETVAVQARRTFEIANRDAE
                     QWLRAVMAPLETQVREYQLQLKRRLESVKRIHQATDTLENRVNELQQAETQLVRQLAE
                     LQALSERVEHALDLPPIGEPAQPLAA"
     gene            complement(14718..15530)
                     /gene="mutM"
                     /locus_tag="PKX01_06735"
     CDS             complement(14718..15530)
                     /gene="mutM"
                     /locus_tag="PKX01_06735"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HMZ75858.1"
                     /translation="MPELPEVETTRRGIAPELTGRTATGALIRNGRLRLPVPEDLDAC
                     ITAQPLLAVERRAKYLLLRFTSGTVLIHLGMSGSLRVLSLEQPPGKHDHVDLCFGERI
                     LRLRDPRRFGLVLWYTGPDDAHPLLAGLGLEPLSEAFDGTWMHRASRGRATPVKLFLM
                     DGRQVVGVGNIYASESLFRAGIDPTTPAGSLGPRRCARLVNAIRETLTDALAAGGSSL
                     RDFVGAGGQPGYFQQQYFVYGRNGEPCRQCATPIRKRIMGQRATFYCPRCQR"
     gene            15711..17435
                     /locus_tag="PKX01_06740"
     CDS             15711..17435
                     /locus_tag="PKX01_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ75859.1"
                     /translation="MSPRPSAFPVRVLVAAACFATAVPAAAQLGGDDAETSSAAALPG
                     QVLTPQIFYQVMLAEVAAARGQGAAGAQAYLDLARRTRDPRLARRAAELAALSRKADL
                     AGEAAQLWLELDPASPQAQQVAAGFMASSAARIDALRSQLAKALATQGDQTGPVLLSL
                     NRGLARIPDKALVRTLVFELTEPYLLLPEAHFARSNAAYVAGDARGALTAIDSALAIR
                     PDWEQAVLLKAQLLQEVVAPAQGQTYLKDYLAAHPDGREARLTLARMLVADRQFGAAL
                     EQFRALLQRLPDDRDVVHATALLSMQQGDTASAEKLFRHLLDLGFGDADQIRAYLGQI
                     AEDGKRPDEALGWYRSVKPGTQYLPAQTHIAQILAAQGRLGEARQHLHQGARENPADR
                     VAYLLTEAQILRDAGRNAESFELLDDALRTRPDDPDLLYESALMAERLGHIDVMEGRL
                     RKLIALKPEHAHAYNALGYSLADRGLRLDEAETLIARGLALSPEDPFILDSLGWVRYR
                     KGDLAGALAALQKAYGVRADPEIAAHLGEVLWTLGRRDEAAQTWRDALKAHPDNTVLS
                     DVLKKFKP"
     gene            17432..18010
                     /locus_tag="PKX01_06745"
     CDS             17432..18010
                     /locus_tag="PKX01_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HMZ75860.1"
                     /translation="MRRACHVLIATAVLALAGCAGVPSGPPVGVPGVRPAEPLQRFAL
                     EGRLQVKDGERLAAVGVSWRHERGHDEWLFTGPLGQGLARLESGPEGARLSFADGRHQ
                     DAAAATELAAAVLGVEAPFEALPAWVTARLREGAEARAVDAVARPTRVVDAGWTVEYV
                     DYVGPEPDARPRRIDIHRGDTRLRLVVDDWNP"
     gene            18012..18872
                     /gene="ispE"
                     /locus_tag="PKX01_06750"
     CDS             18012..18872
                     /gene="ispE"
                     /locus_tag="PKX01_06750"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HMZ75861.1"
                     /translation="MNPTDFDPSMSVCVPAPAKINLFLHIVGRRADGYHLLQTAFRML
                     DWGDEITLKRRDDGVIRRTADVPGVPAERDLVVRAAQALQAASGTRYGADIGVSKRIP
                     MGGGLGGGSSDAASVLLALNRLWGCGLSRTRLQEIGLTLGADVPFFIFGETAFAEGVG
                     EALVPLAVPPAWYVIVAPPVEVPTAKIFSAPGLTRDSELIKMRDFATDTTRNDMQATV
                     CAMFPEVAEALVWLSSRGAARMSGSGACLFAAFSSKAQAEQVAAAAPQGLKVWVAPGL
                     DRHPLWAWVG"
     gene            18891..18967
                     /locus_tag="PKX01_06755"
     tRNA            18891..18967
                     /locus_tag="PKX01_06755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18925..18927,aa:Gln,seq:ttg)
     gene            19044..19994
                     /locus_tag="PKX01_06760"
     CDS             19044..19994
                     /locus_tag="PKX01_06760"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMZ75862.1"
                     /translation="MASGSLMVFTGNANPKLAADVVRRLGRSLGSATVGRFSDGEVNV
                     ELLENVRGKDVFVLQPTGAPTNDNIMELMIMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKVVANMLQAVGVQRVLTVDLHADQIQGFFDIPVDNIYAAPVLLDDLEK
                     QKYDDLLVVSPDVGGVVRARAFAKRLECDLAIIDKRRPKANVSEVMNIIGEVDGRTCV
                     IMDDIVDTAGTLCKAAQALKENGAKRVMAYCTHAVLSGPAVSRIMDSDLDELVVTDTI
                     PLTDEARACSRIRTVSIASLLADTIVRISNEESVSSLFTE"
     gene            20081..20686
                     /locus_tag="PKX01_06765"
     CDS             20081..20686
                     /locus_tag="PKX01_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HMZ75863.1"
                     /translation="MTIQFNASKREAQGSSASRRLRRAGRVPAIIYGGEVAPLQVELD
                     HNEIFHLLRNEAFHSSVLTALVDGAAQAVILRDTQWHPYKQQVLHLDFQRVDSTHKIH
                     VKVPLHFVNADIAPGVKLEGGIVSHVMTELDLECLPGDLPEFVEVDLKDLKGGASIHV
                     SHLPLPAGVKALTHGEDPVVATILAVRGGGEDEAAAETPAA"
     gene            20718..21314
                     /gene="pth"
                     /locus_tag="PKX01_06770"
     CDS             20718..21314
                     /gene="pth"
                     /locus_tag="PKX01_06770"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ75864.1"
                     /translation="MSQTAPRLVVGLGNPGAEYSETRHNAGFWFCERLARELGASFSK
                     EARFHGLAAHARDAGVWLLMPQTFMNRSGQAVAALARFYRIPPAEILVVHDELDIPPA
                     HLRLKFGGGLGGHNGLKDITAHLGTQDYWRLRIGIGHPGDRNEVVNFVLKPPRREEAA
                     LIDDALDRALAVWPRIAAGDWNAATQRINARPPGDAKK"
     gene            21463..23085
                     /locus_tag="PKX01_06775"
     CDS             21463..23085
                     /locus_tag="PKX01_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ75865.1"
                     /translation="MRRLADTPIWLRLTAAIWLMLVIAWGGMIVWETRTNRETAVAQA
                     EDFARSIHEMTMAGLTGMMITGTVGQREVFLDQIKQLSIIHDLQVLRGDAVSRQFGPG
                     TRPQPPLDADEQAVLATGQAVSRIQTDPRLGEYLRVIRPAIASDNYLGKNCIGCHLVP
                     AGTPLGLVSMKVSLDKVNEAVESFRWKSIISALVLSLPLILFVTYFIRSFVARPLADM
                     NRSLHEIAKGEGDLTRRLPVRGQDEIGQAADAFNAMLGTVASLVRRVSDSASRVTASS
                     HQLTMSASRVAEGARAQCEQSASAASEVETMASDIAHVAVSTEDVHQRSRESLRRSQD
                     GSRSLDMLVGEVGQAEASVRQMADSVGQFVESTSAITTMTQEVREIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAEKSARSAGEIDAVTSRLSQQSVAVRQAIHEGLEH
                     LESSRVAVGEVSAVIVSANESVEAVGRGLDEIAGATERQRAASRRVAESIDAMAEMAR
                     ASNGAVESTADAARDLERLASELQETVARFRV"
     gene            23143..23985
                     /locus_tag="PKX01_06780"
     CDS             23143..23985
                     /locus_tag="PKX01_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ75866.1"
                     /translation="MQHFIADDGECLHVQVSGEGPPVILLHGWTSSHRDWNPFVDVLS
                     ADHHVLRWDARGHATHALQAATAPTVQRMARDLANLLDHWHLERAVLVGHSMGALTLW
                     QYLQDFGSDRVAGVVIVDQSPRLVTDATWRNGIYGDFDTARNAAFIAELEADFGEAVL
                     RLTAFGRNGEVRRRYTDQPESFDNARRWLGRLHHAALIACWRTLAAADYRAVLPAIDV
                     PALLVYGAESNFYSRETAEWVAGAIPDARLTVYPGADHSPHLHDRARFGADLDDFLAR
                     VFPR"
     gene            complement(24031..24987)
                     /locus_tag="PKX01_06785"
     CDS             complement(24031..24987)
                     /locus_tag="PKX01_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMZ75867.1"
                     /translation="MNASRQPTALIKPEVLYTALFIVGLIAPFAVYPTFLMKLFCFAL
                     FASAFNLLLGFAGLLSFGHAAFLGSAGYVCGMMVRDVGVTPEVGILAGTAAAGLLGWL
                     FGVLAIRRTGIYFAMITLALAQMVYFIVLQVKATGGEDGLQGVPRGHLFGFIDLTDNL
                     AMYYLVYGVFALGFFIIHRTIHSPFGQILKAIRENEPRAISLGYDVSRFKLIAFVISA
                     SLAGLAGATKTLVFQLVSLSDVHWHMSGEVVLMTLLGGLGTILGPSVGSGVIVTLQNE
                     LADKVGSWVTVIMGAIFVLCVLAFRRGLVGEIQALIHKAGIR"
     gene            complement(25012..25893)
                     /locus_tag="PKX01_06790"
     CDS             complement(25012..25893)
                     /locus_tag="PKX01_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMZ75868.1"
                     /translation="MEIFGVPTALLGSQLLVGLINGSFYAILSLGLAIIFGLLNIINF
                     SHGAQYMLGAFVAWIALTRFEINYWVMLIAAPLLVGAFGVVLERTMLRKLYKLDHLYG
                     LLLTFGLALIIEGVFRDQFGISGESYDVPEALAGGINLGFMFLPIYRAWVIVASLTVC
                     FGTWFMIEKTKLGAYLRAGTENPQIVQALGINVPLLITFTYGYGVALAAFAGVLAAPI
                     FQVNPLMGSNLIIVVFAVVVIGGMGSIMGSILTGIGLGLIEGLTRVFYPEGSAVVVFV
                     IMVIVLLVRPAGLFGKA"
     gene            complement(25950..27167)
                     /locus_tag="PKX01_06795"
     CDS             complement(25950..27167)
                     /locus_tag="PKX01_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ75869.1"
                     /translation="MTTSKKLLTAACTAALATLSAGAHAQISGGVVKIGVLTDLSGTY
                     SDLAGSGAVLATKMAVEDFIAKEKPTFKIEVVSADHQNKADIASNKAREWFEREGVDV
                     ASELVTTSVALAVMKIAKEKDRIALMSGPASTPITNEQCNDVTVHYTYDTYALANGTA
                     KAVTKQGGKTWFFLTADYAFGQALEKDASAVVTANGGQVLGSVRHPFPGSDFSSFLLK
                     AQASGAQVIGLANAGADTTNSIKQAAEFGITPKQSLAGLLMFITDIHALGLKATQGMY
                     LTEGFYWDLNDQTREWSRRFMGAQKKMPTMVQAGQYSSVYHYLKAVKAAGTDDTKKVM
                     EMMKKTPINDFFAKNGRIREDGRMVHDMYLMQVKKPAESKYPWDYYNVRQVIPADEAF
                     QPLSLSRCPLVKK"
     gene            complement(27203..27925)
                     /locus_tag="PKX01_06800"
     CDS             complement(27203..27925)
                     /locus_tag="PKX01_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75870.1"
                     /translation="MTSAFDPKPEMLRVRDLHAYYGESHILHGIDFSVGRGECVTLLG
                     RNGAGRTTTLKALLGLVGRRTGSIMLNGKEIVAEPTHRIAGYGVGYVPEERAIFASLS
                     AEENLLLPPQVASGGMTVDEIYAMFPNLRERRSSPGTKLSGGEQQMLAMARVLRTGAK
                     LLLLDEITEGLAPVIVKKLGEVIVELKSRGFTIILVEQNFRFAAPLADRHYVLEHGEI
                     LATIPQEELPGRMDWLHQTLGV"
     gene            complement(27939..28706)
                     /locus_tag="PKX01_06805"
     CDS             complement(27939..28706)
                     /locus_tag="PKX01_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75871.1"
                     /translation="MSDYILETAGLTKEFKGFTAVSNVDLKVRRGHIHALIGPNGAGK
                     TTVFNLLTKFLPPTRGTIHFDGRDITREAPAVTARRGLVRSFQISATFPHLTVMENVR
                     IGLQRKLGTEFHFWRSEKSLDVLNDRAMELLEAVDLTRFADTVTAEMPYGRKRALEIA
                     TTLALEPAMMLLDEPTQGMGHEDIGRIVELIRKVSVDRTILMVEHNLSVVSHLCDRIT
                     VLQRGSILAEGPYEDVSKNPQVLEAYVGSSDMNDAHH"
BASE COUNT         4629 a         9127 c        10779 g         4623 t
ORIGIN      
        1 ctcggcgcgg gcgatctcgg cggcggcggc ggcgtcgcgg gcatcgcggc tggcgtcgag
       61 gaaggccagc gccggggcca gtgcgcgacc atagagggcg gcccaggcgg ggttgggggt
      121 gttgtgctgg cgccagtcga gggcgaactg gagttccggg tcgcgccaca ggctggcttc
      181 accgcgggcg tgcaggtcgg cggcgtcggc gaggcggcgc agcatgcggg cggcctcggc
      241 ctcccgctcg ccccaggcgc gcaggcggtc ccagatgcgc atgaggctct cgtgggccag
      301 gtccacgacg gtgtcggcgg tgagggcgac gccttcgggc ggcatcagga aggcggcgcc
      361 cggcgcgcga aagggggcaa gggtggtggc gagctcctcc gctggcgcgc cgccgagggc
      421 caccagatcg gcaaaacggc aggggcgacg gacgccccgg tgatcgctgc cggtatcggt
      481 gagggcgcgg aaggcctggg cgcacaggcg ctggcggccc ggcggcaggg cggcgtagag
      541 ctcctcggcg tgctggttga gggcgtgggc gagggtgccg acggcgtcgt agtggcgcgc
      601 ctcgatgggg ctgggcgtgc ggctggcgcg ccagtgcgcc caggtgcggg cgagggcgtg
      661 ctggagcagg gagagctggt ccgggtcgtc gccgacgtca ttcacgaggc gggtgagcag
      721 ggtgggggcg atgtcggcgc cgcacacccg cgccggcccg gcgatggcgg cgcggcgttc
      781 gtcgcggctc atgcgcggca ccagatattg cccctggttg atggcttccg gcaggccggc
      841 gaagcgggcg cagtcgccga ggaagtcgga gcgcatggtg agcacgacgt ggatcgggac
      901 gtcctgggcg cgggcttcga ggaggagatt gacgaaggcg gtggcggggt cgccgtcggc
      961 gtcgggctgc gcatcgccac gggggccgag gccgcggtag cggaacagtt cttcgaactg
     1021 gtcgacgatg acgaggagct gggtgcccgc gggcaggccg gcctggagga aggcttcgac
     1081 gaggccccgg cggctcatgg cgagggtgga ttcgatgagc tcggcgaggc ggaaggggct
     1141 gtcgggcaga tcccggtgaa gggcgccggg ggcggccagg gactcggcga gggcgcgcag
     1201 gggctggctg ccggggcggc aggtggccat ccgccaggcg ctgccggcct gggcgaggag
     1261 gccccggtgc agggccgggc gtagaccgca attgacgagg gaggacttgc cgcagccgga
     1321 gctgccgatc acggcgagga agcggccgcg ggcgagggtg tccacgaggg cgtccacctg
     1381 ggcctcgcgg ccgaagaaga ggtgttcctc gtcttcgcgg aaggggcgga tgcccggata
     1441 aggcgtatcg aggcgcaggc gggtgaagct catggggcct ccgtgccgag ggccgccagc
     1501 agaggggcga ggacggcggc gtcgagcccg tcgagggcat ccagcgtatc ggcctcggcg
     1561 agggcgcgga ggaacgcctt gtcatcgccg agcggcgggg cgaggagcac gaactggccc
     1621 cgcagggggc gctcccgctg gctcgcgcag agcttctgga ggtcgttgcc ctgatggtac
     1681 ttccacgcct cgtcgccggc gccgtaccac aggacgagga cgtcgcagtc gagggcgagc
     1741 tggcggttgg cttcgcgcac ggcgccggca tcgccgctga agacgggcag ggtgacctcc
     1801 acgccatggg cgcgcagcag gcggatgaag ggcaggatgt ccttgcggtc gcgggcgtcg
     1861 cataccaggt gcacgcggcg tgcgctgacg gagggcgcgg cgggcggtgc ctcgggcggc
     1921 ttgagggcgg cgtggatggc ggccttgagg acttccaggt cgccttccac gagatcggcg
     1981 ccggcctgca gggcggcgtc ctggcgcagg gcgtcgagga aggcctgctg ggccggcagg
     2041 ggcgaggcga gtcccggcgg cagaccgatc acccggcgca ggccggcgct gcggctgcgg
     2101 gccgcagcgc gttcgttctg caggacaacg accgactgct ggcgcgcacc gtccgggatg
     2161 cgcccgctgc cgctgccgat gaggtggatg gacagcacgc agcgggccag cagggcgtct
     2221 acggcagcca cgtgctcgtc ctcgtcgtcg ggcaggcgct ggtcggggag gacggtgtag
     2281 ccgcagcagg cgagctcggc ctcgatgcgt tcgcgggcct cgcggcggtc gcggctgcat
     2341 tcggcgagga acacggcggg gcgctctgcg gcggggggcg ttgcgttgcc gtcattggcg
     2401 gcgggcgtat cggccagggc gttgatctgg gcggccaggt cccaggcgag cttgttggcc
     2461 ttgcgcagga aggcctggcg gggctgctcg ccgtaggcgg ggtcgagttc ccgcggggtg
     2521 cggtcctcat cgaattcgta gaactcgtag ccgaggagct gggccgccgc cgggggcagg
     2581 aggccatcgc cgtccacggg cagcttgagg accttgatga ggcgggactt gttatccacg
     2641 aagaggctgt gttcggcttc ggcccgttcg cagaagctgc ggatctcgcg cgcgcaccac
     2701 tcggacttga ggtagcgcgg ggtgaggatg gagacgaaca gggcggtgcc ggcaagctgg
     2761 ccgaggatct cgtcggagaa gacgtcgttg ccacgcagct tgtcgtcgcg ccagatgtcg
     2821 gcgggggcgc cgaggcgctg gctgaggagg gtctgcaggg tggcgtggaa gaggctgacc
     2881 cagccggcct ggtccgccgt gaggggttgg ttgtccaggt gggcatagct gatgaagatc
     2941 tggcgggcgt acttcatcgg ggcagacctc cgggcagggc gaggccgggg ggcaggaagg
     3001 cggcgagctg cgcccggtag gcgtcggcct gccacaggcg caggccgcgg gcggcgaagg
     3061 cgcggaagtc gtcggggccg ggcagggagc ggtagcggcg caggcggcgg tcctggggtg
     3121 tgccgggggg acgctcgccg gcggcgcagg cggcgatcac ggcctgtaca gtgggcaggg
     3181 ccagggcata gagctcgccg atctgggtgt agaggccgcc ggcagggtcg aagggggtcc
     3241 ggtggacggc caccaggggg ccgtcgtcga tgcccgcggt aatccagtgg acgctgcagg
     3301 cgagctgcgt ctcgccttcg aggacgctgc gcaggggggc gtgcaggccg gcgtagcggg
     3361 gcaggtggcc ggggtggagg ttgagcaggc cgaagcgcgg cacggcgagg gcggcggcgt
     3421 cgaagaggtg gctgaagcgg gcgacaatga ccaggtccgg agcgagggcg gcgagccgcg
     3481 tggcggcgtc ggggagttcg tcgaacacac tgaggttcac cccgtgacgg cgggccaggc
     3541 cgtgaaagct ggcggccggg gcggtgtcgg cgagcgccgg cggcagggca tccacgaggg
     3601 ggaagagcag gtccaccggc aggtggcgtt cgaggcaggc cagttcggcg agctcgggca
     3661 actcggcctc ggccgggcgc tgcttgtccg acagccacac gccgacgagt tcgtggccgg
     3721 tcaggctggg cagcaggcgg ttgaggaggc gcgcgccatg gaggtcgcgc ttggtgcaga
     3781 cgagcaggcg catggtcagc ggtccgctcc gtccaggccg acgatctcgg ccacgtcgag
     3841 gagcttgagg ggcgggtagc gtccgagctg gagtgccacc ggcaggttga tgaggtagga
     3901 gaagttgcgt ggcagttcga ccgggatggc cgccggcgag actttgtccc gcaggatgcg
     3961 cagggcctgg gcggcggtga agcggcccac gtcctcgtac ttgttgacca cgccgaagag
     4021 ggcgcggccg ttctgcacgg gggcctcggc agcggcgaaa acgggcatgc cgcgggccag
     4081 ggcgccgtcg gtgaggacgt cgcggcggga attgaggaag ctgtcggggc cctggtacag
     4141 gagatccacg ccggcgccct tgagggcatc cagcagggcc ggcagggcgg tggcgtccgg
     4201 cgtgccggcg gcggagacgg gcagggcgcg ctccaccagg gtgaaaccct gactgggcgc
     4261 cagggcgcgc agggcgtcgc gggtggtgat ggagttggac tcggtctcgt tgaggatgta
     4321 gccgagcgta cggaacgcca gatagctgtg tgccgccttg atctgcgtct ctacgggcaa
     4381 caggtagagg gtgccggtga gcgtgcggcg gggtttgtcg agacgctgca cgatgcccgc
     4441 ggcgaccggg tcggagacga tcatgaagac caccgggacg tccgtgaggt ggcgcctggg
     4501 atccacggcg ttccagcgtc cggcggcctc ttcggtgacg gtggtgcccc aggtgacgac
     4561 cagatcgggc ttctcacggc gcagttcctg cacgaagccg gcgatgcggc ggcggtcctg
     4621 ggcggcgtcc cgcagggtga attcggcggc gatgccgtgc tggcggaagt aggcctggaa
     4681 gccgcggcag gcctcctcgc agccgcggta gaggagcatg gcgatgcggt agggcggatg
     4741 ttcggccggg gctgcgtgcg cggcgccctg ggccgtcagg agcacggcaa gggcggcgag
     4801 ggcgggggcg aggtacgcga ggcgcttcac gaaggctcct tgtccggcgt gtcgagggac
     4861 gctgcagccc gcgcccacag ggcgaaggcc agggtggcga ccagcatcgt cgcgcccacg
     4921 gtggccatcg cgccgcccca ggcggtgacg gccagcaggc tgccgacaaa cagcgggccg
     4981 atcaccgcgc cgaggcgttc gacggtgcgc agcagcacga tggcggccgg gccgtcgcgg
     5041 ctggccaggg cctgcaaggc cgctgccgac aggccggtgc caaggcccag ggcgacgatg
     5101 ccggcgaaca gggtctcgat ccggtccagg aggcccagta cgccaccggc gccgatcacc
     5161 agcccccccg cgatgaccag gctgcgctgc cagccgaaac ggtccgacag ccaggatgcc
     5221 agcggattgg cggccgccac cagcacaaaa tacagcatca tggcccgtcc ggtcgacgcg
     5281 gggctgtagt ctgcaccggc cagggcgagg ggcacgaggt agaacaggaa gccggcaagc
     5341 accaccttgg ccggcaaggc ggcggtgagg agcgggatga cgacgcgtcc gcgggtcagc
     5401 aggggcagca ggcgcggtgc cggggcgccg gctgcgggcg gcgcgacggg tacggcgagg
     5461 cgctggacga ggaaggccag gccgctcatg gcggcggcgg cggcgaacac cgcggtggcg
     5521 ccgaagcgtt cggcgagcag cccgcccacg gggctgccgc agatcgcggc ggtctgcacg
     5581 gcgccgacga agagggcgag ggcacgggtg cgctcggagg ccgtggccgt gctcagaaag
     5641 tgctgctgga tggccagggt gccgatggcg tagccggcgg cgcacaggca gcgggccgcc
     5701 acgaaggcgc cgtagccgcc gccggccatc gccccgagga agcccgccgc cgacaggccg
     5761 gcgccgaggg cgaaggtgcg tgccacgccg aaacgcaccg cccagtgtcc ggcaaagggg
     5821 gtgagcaggg cgaagctggc catgtaggcg gagatcggca gcccgaccag ggggcccgtg
     5881 agggcgtctt cggcggccgt ctgcgcgacc agggcacgga tgtgcacggg caggaaggcg
     5941 cgggtgagtt cctcggcgag gatcagcagg aacaccgcga ggcgcgccgg gccgaggcgc
     6001 tcccgcgggc cggcggcaaa gcgggcgctg ccgagaatcg cgcagagggc ctcattgaag
     6061 agcaccagcg ccaccaggaa gacgatggcg aggtccacgg ccagggcgcg cagttcccgc
     6121 gcgaccaccc cggcgtcggt gacggcgacg agcgtggcac cggtcgcgcg gacacccgca
     6181 gagatcggga cctccagcgc gtgaccggct tcgcccggac ggctcgccgc gtagcggtgc
     6241 gtgggcccct gcaggcgcag ggcgaggact tccgggtgct gggcgagggc gtcggtgaag
     6301 taggcttcga cgccgttcag gcgctcgaag ggaatgccca gggccagcgc ggcgtcgatg
     6361 cgcgtggaga gaaaacaggc gagggcatgg gccttgtcgt ccagcgcggg cgccagacgt
     6421 tcgctgaagc tgggcagcgc cgcgaggctc aggtgcaggc tgccggcggt ggccaggcac
     6481 agcgccgcgc cgaggacgcg gcgacgcagg ctgaagggcc cgggcaggcg catggcgaga
     6541 acgagcagca ccggcagggc gacggcgacg agcccgaggg tgagggccaa acgcgggccg
     6601 gccaccgcgc gggccgcttc gatgcgagcg tcgagcgctg cgctgcgcac cgcgatcagc
     6661 acgctgccca cagattcgcc ggtgctgtcc tggagtgtcc ggccgatcca catgtacccc
     6721 agaggggcgg tggcgtgcca caggccgcgg gccgcgcgcg gcggcgatgg cacgggcagg
     6781 tcggtcggcc cggtggcgaa gagacggcgc ccgtcctcgt cgagcacctg gatggccgtc
     6841 acgtcctggg cgctggcggc aagggccggc aggcgggcgg caagtgcgcg gaattcgccc
     6901 agctgcaggc cgttggcgga ggcacgctgg aagctgctgt ggatctgttc ggcgagcagg
     6961 ccgatgcggg agacggtgat gccggtgagg agatcccgct ccttgtggaa cagcagcacc
     7021 gcctgcaggc tcagggccag accgagggcc gccagcagga ggaggccgat gcgcagccat
     7081 tgccggagat ccggcggcgg ggcggccggc agcgacggtg gttcggcgcg ggggggcatc
     7141 ctcggctctc cttcggtgcg acggcgggcc gccggcgcgg ggcatgcggc aaggataggc
     7201 gaagacgccg ggcgccgcac gcctttgcgg cgcagggcgc gctggggcca cgcgccaggg
     7261 cacccgcccg gcgccgggac tcagcctgag gcgatcaggc cggggggtcg gccggtgcgc
     7321 cgcctgccgg cgcgaacagg gcgtccacga actccctggc gcggaaggtc tgcaggtcgt
     7381 cgatctgttc gcccacgccg atgaagcgca gcggcttggg gcactggcgg ccgatggcgg
     7441 ccaccacgcc gcccttggcg gtgccgtcga gcttggtgac gacgaggccg gtgacgccga
     7501 tggcggcatc gaaggccttg acctgctgca gggcgttctg gccgatgttg gcgtcgagca
     7561 ccagcagtac ctcgtggggg ccggacggct cggccttctg gatgacccga cgcaccttgg
     7621 cgatttcttc catcaggtgc agctgggtgg gcagacggcc ggccgtgtcg gccaggacga
     7681 tgtcgatgcc acgggcgcgg gcggcgttga tggcgtcgaa caccactgcg gccgggtcgc
     7741 cggattcctg ggcgatgaca gccacgccgt tgcgctcacc ccaggtcatg agctgctccc
     7801 gcgcggcggc acggaaggtg tcgccggcgg ccagcaggac gctcttgccc tggttctgga
     7861 agtgcttggc gagcttgccg atggaggtgg tcttgccgga gccgttcacc cccgcgatca
     7921 tgatgatgta gggcgaatgg ccgctgatct cgaggggctt ctcgaggggc gcgaggatgg
     7981 acagcaggcc ttcggcgagg gcgccctgga gctggtcggc ggtttccagc ttgtcccgct
     8041 tccagcggcg gcgcagttcg tcgatgaggt actgggtcgc ctcgacgccg cagtcggcca
     8101 tcagcagcgt ggattccagt tcttcaagga gttcctcgtc gatcttgcgg cgggagaaca
     8161 ggctggacag gccgccgccg atctgctggc gggtgcggga cagaccggcg cgcaggcggt
     8221 ccatccagga tttcttttcg ggcgcagcag ccgggctcgc cggcgtcgcg acgggcgcgt
     8281 ccggcgaggc gggcgcgtcg tccgccttgg gggtcttctt caggaaacca aacatggaga
     8341 gccgggtcag agtgagggtc gatcgggtcg gccacgcggg tgaaagccct tgcgtgtcct
     8401 gttggccggg tcgccggatg ccgctatgat gccgcgccac acgccggcca gcggccaccg
     8461 tccctcaatt ttaccagatg cttcaagagc ctatgatgaa tccccgcgga cgcctcgcgt
     8521 ccaccctggc ggccctgtgc gccgtggccg gtctggccgt tgtcgccccg gcccgggcca
     8581 atcccttcga gacggtactc cccaacggca tgaagctggt cgtcaaggaa gaccggcggg
     8641 cgccctcggt ggtccacatg gtgtggtacc gggtgggggc gatggacgag gtggatggca
     8701 cctcgggcgt ggcccacgtg ctggagcaca tgatgttcaa gggcacgaag gccgtcggcc
     8761 ccggagagtt caacaagcgc gtcgccgcgg ccggcggccg tgacaacgcg ttcaccagct
     8821 acgactacac cgcgtatttc cagcaggtgc ctaaggacgc gctgcccggg atgatggcgc
     8881 tggaggccga tcgcatggcc catctgcgca ttaccgacgc cgaattccgc aaagaactgg
     8941 aagtcgtcaa ggaggagcgc cgcctgcgca ccgacgacaa gccccgcgcg ctggtgatcg
     9001 agcagctcat ggcgagcgcc ttccaggcgc atccgtatcg ccgtcccatc atcggctgga
     9061 tgaacgacct ggacgccatg accgcccagg acgcccgcga ctggtacgtg cgctggtacg
     9121 tccccaacaa cgcgaccctg gtcgtggtgg gcgatgtgga tcacgcggcg gtgttcaggg
     9181 aggccgcagc cacttacggc cgcgtgtcgg cccgcccgct gcccgagcgc aagcccctgc
     9241 gcgaacccga acagagcggt ccgcgcagca ccgtggtcaa ggcgcctgcc gagctgcctt
     9301 acgtggcgct ggcctggcac gtgccgaccc tgcgcgacgt gaaggctgat ggcgactact
     9361 acgccctgca ggtgctggcg gccgtgctgg acggctacga gggcgcccgc ctggccaaga
     9421 acgtggtgcg tggcagccgc gtcgcggtga gcgccggtgc cggctacgac gggaccgccc
     9481 gcggcgagtc cctgttcgtg ctcgatggcg cgccagcgcc gggcaagacg gtagccgatg
     9541 tggaggcggc cctgcgcggc gaactcgccc gcatccagaa cgagggtgtg tccgaggacg
     9601 agttgcgccg ggtcaaggtg caggccgtgg cggcccaggt gtacaagcgc gactccctga
     9661 tgggccaggc catggagatc ggcatggacg agatggtcgg gctgtcgtgg aaggatgacg
     9721 actaccagct ggcgcggctg cgcgccgtga cggccgccga ggtccaggcc gtcgcccgca
     9781 agtacttctc cgacgacggc gtgaccgtcg cccgtctcga cccgctgccg gtggatccgg
     9841 aagcccgcgc ccgtgcggcc gctgcggcgc cccatcgcca ctgatcgctt tctgcaagga
     9901 ctgcccatga tgatttccgt atcccgtttc ggggccgcgc tcgccttgac gctcttcggt
     9961 ctgtgtggcg gcgcccacgc cggcccggcc atccagacct ggtcaacacc caacggcgcc
    10021 cgcgtcttct atgtggaaag ccacgccctg ccgctggtgg atgtgcaggt ggatttccgc
    10081 gccggcggcg ccgaatcgcc ggccggcaag accggcgtgg cgggcctcac ccacgctctg
    10141 ctcgacaacg gcgccggcga tctggacgag gaggccatcg ccaaccgcat cgccgacctg
    10201 ggcgcccaga tggggggcag cgccgacatg gaccgggcgt ccatcaccct gcgcaccctg
    10261 tccagcgcag ccgagcggga cggcgcggtg gatctgctcg ccacggtgat ccagcatccc
    10321 acctttccgg cggccagctt cgaacgggag aaggcgcgca ccctcgccgg cctgaaggaa
    10381 tccgacaccc agccggacgc gatcctgtcg cgccgcttcg cggctgcggt gtttcccggc
    10441 catccctacg gccgcctgcc caccgtggac agtgtggcgg cgctgaatcg cgacgacctg
    10501 gctgccttcc atgcccgcac ctacaccgcg gcgcgggcgg tggtgagcat cgttggtgat
    10561 gtgagccgtg ccgacgccga agcgatggca gcgaagctca cggccgatct gccgcggggc
    10621 gaggcgccgg cgacctccgt caacaccgtg ctgcccgagg cgcagacgat ccgcgtggcg
    10681 cacccgtcgg cccaggccca catcgccatc ggccagcccg gcatccgccg cggcgatccg
    10741 gacttcttcc cgctgctggt gggcaactac gtccttggtg gcggcggctt cgtgtcgcgc
    10801 ctgaccaagg aggtgcgcga gaagcgcggt tacgcctaca gcgtgttcag ctacttcatg
    10861 ccccagcgcg acgtgggccc cttcgagatc ggtctgcaga ccaagggcag ccagaccgac
    10921 gacgccctgc aggtggtgaa tgcgaccctc gccgaattcc tggacaaggg gcctaccggg
    10981 gccgaactcc aggccgccaa ggacaacctg gtgaatggct tcgccctgcg cctcgattcc
    11041 aatcgcaagg tgctggagca ggtcgcggtg atcggcggct tcggcctgcc gctggactac
    11101 ctggaccgct ttccggacaa ggtgcgcgcg gtgacggccg ctcaggtgcg cgacgccttc
    11161 cggcgccacg tctcgccggc gcacctcgtg accgtggtgg tggggggtga cggcgacaag
    11221 gcggccgcgc cgcgggccga ggtggcccgt tgagccgcat ccgcatcatc ggcggcgaat
    11281 ggcgctcgcg cctgatcgac gtgagcaccg tcaagggcct gcgccccacg ccggatcggg
    11341 tacgcgaaac cctgttcaat tggctgggac aggatctgga cggacggacc tgcctggatt
    11401 tgtttgccgg cagcggggcg ctgggcttcg aggcggcctc gcgcggtgct gcggccgtga
    11461 cgatggtcga acaggacccc gtggcttttg ccgcgctgca gaagaacaca aaaaccctac
    11521 ttgcgtcgcg tgtagagctt gtccggggcg atgcgctaaa attcgccggc tccgcctccc
    11581 gccggttcga cgtggtcttt ttcgacccac cctaccgcgc cggctggatc gaccggatgg
    11641 ccccgttcct gcccgatctg ctgaccgaac atggcgtgat ctacgtggaa gcggaataca
    11701 aaatcgaaaa tttcgaccga ttgacacgca tcaagcacgg cgtggccggg caggtgtatt
    11761 accacctctt cgccgcccag gcgtgaattt caccggtcgc gcagacaagg ggatacatca
    11821 tgcgggactc ggtcgcggtc tacccaggta ccttcgatcc gttcacccgt gggcacgagg
    11881 atctcgtccg gcgggcggcc cggctgttcg accgggtcat cgtcggcgtg gcggcgagcg
    11941 gcagcaaggg accgatcttt tccatggaag agcgggtcga gatcgcccgg gaagtcctga
    12001 cggcctatcc caacgtggaa gtgcagggat tttcctgcct gctgatggaa ttcctgcgcc
    12061 gcaacgaggc ccgcgtgatc ctgcggggac tgcgcgccgt atcggatttc gagtacgaat
    12121 tccagatggc gggcatgaac cgcaagctct tcgcggatgt ggaaaccgtg tttctgacgc
    12181 cggcggatga atacatgttc atctccgcca ccatggtgcg ggaaatcgcc cgcctcggcg
    12241 gcgacgtcag caagtttgtt cagccgggcg tgaatgcgcg gctggtgcag aagatcaact
    12301 acagcaaatg acgaggaagt tagcgatatg gccttgatga ttaccgacga atgcatcaac
    12361 tgcgacgtct gtgagccgga atgccccaat ggtgccattt cccagggcga ggagatctac
    12421 cagatcgacc ccaacaagtg caccgagtgc gtcggccatt tcgacgagcc ccagtgccag
    12481 caggtctgcc cggtggattg catccccttc aacccggagt ttccggaaac gaaagacgat
    12541 ctgatggaga agttcctcaa gctcacggcc gacaagtaag acgccgcctg accgccgaag
    12601 gacagacgcc ggccgtgcag tgatgcacgg ccggcgtcgt ttgatggtcc gggccggact
    12661 caggccgcga gcggctgcgc cggttcgccg atgggcggca gatcgagggc gtgctcgacg
    12721 cgctcggaga gggcctgcag ttcggctagc tgccggacga gctgggtttc ggcctgctgc
    12781 agttcgttga cacgattttc aagggtgtcg gtggcctggt gaatgcgctt gacgctttcc
    12841 aggcggcgct tgagctggag ctggtattcc cgcacctggg tttcgagcgg cgccatcacg
    12901 gcccgcagcc attgctccgc gtcccggttg gcgatctcga aggtgcggcg ggcctgcacc
    12961 gcgacggtct cgaagaactt ctgggtcagg gtgtgcttct cggtggtgac gatgttcagc
    13021 accgtgtcga actggctgtt gaaggcgtct tcgaggcgct cgatctcctt ctcatagcgc
    13081 agggtggaga aggacgaggg tgcggccagc ttgagcccgt gttccaccgt gaaacgcttg
    13141 tacatggcgt cgagcatctt cgagatctcg gcgatctcgt cggtggattt cttgaggttg
    13201 ttgcgcaggt gctggaagaa gcccttcatg gcgccgcgca ggccccgcga gaaggccgcg
    13261 tcaagcatgg cttcgcgggt gcggcgggtt tcgtcgcgca gggcatccag tcccaggtgg
    13321 ccgaacaggt tgttggacag gttggtgaat acgctgcgca ccgcgtagta cttctgcaag
    13381 ccctgctcga actcgtcctt ttcggagcgc accttgcgca tcatgtattc gatgacgttc
    13441 tggttcttgc cgcgcaggtc ggtgagttcg gtgagctgct cacgcaggcc ggacaggcgg
    13501 gcgccgagca gttcgcggca gcgcgcgatg atgtcctgga cttcaccgta ggtgttgtcg
    13561 cgcacgatgt cccgcttggt gggcaggagc tcggtggaca gggcatattc cagttcggga
    13621 aggcggctgc gggtgaggag gtcgtcgtcg ttgttgacct tggccaccag ccctttctgg
    13681 gccgagaccg ggtagatctg ttccggcgcc acatcgaggg ttgcggcgac gctggctacc
    13741 tgtccggcga tctcgcgctc gatgtccgcc tcgctgcgca ggccgtccca caggccgtcg
    13801 atcttgttga gggcgatgag ccgtccgcgc tggcggcgtc cggcgttgac gtgttcgcgc
    13861 cagacggcca gatcgctctg ggtgacgccc gtgtcggcgg caaggatgaa cagcaccgcg
    13921 tgggcattgg gcagcaggtt gagggtcagc tcgggctcgg cgccgatggc attgagcccc
    13981 ggggtgtcga ggatcaccaa gccctgctcg agcagcgggt ggggaaactg gatgacggca
    14041 tggcgccagg ccgggatctc caccgtgccc cccgtgtccg ggcggatgcc cttctcgccg
    14101 ctctcgtcga tgggaaatcc gagcgcctcg gcctcctcgc gggaggcgat gcgggtttca
    14161 cccacctggg caagggccgc ctggaggctt tccgcggaac tggtgtctag cggacgtacc
    14221 cgccattcgt ccgggaagcg cttgagctcg gagatgctgg cattgctgcg ccggctatcg
    14281 atgggcagca gacggatgca gggcgtatcg ccgtcttccc attgcagttc ggtggggcac
    14341 atggtggtgc gcccggcgct ggaaggcagg atccggttac cgtaacccgc aaagaacacc
    14401 gcgttgatga gttcggactt gcctctggag aattcggcca cgaaggccac gatcagcttg
    14461 tcttcgcgca gccggtcgag caggtactgg aggcgcaggt cggactgggc gtcaccgact
    14521 tcgttctgct gcagccactt gcgcagatcg gcgatcccgg aagccacctc gccgcgccac
    14581 tgggtgtacg cggaaaagtg ctcggcaagc atttgggcgt ctttcacttg cagggtccct
    14641 ggctggtggc cgtgaaatac ggcacatttt gctcttcatt ctaagcgcaa atcgggaaaa
    14701 ttcgcaccgg ccgccgctca tcgctggcaa cggggacaat aaaacgtggc gcgttgcccc
    14761 atgatccgct tgcggatcgg tgtggcgcac tggcggcagg gttctccatt ccggccatag
    14821 acgaaatact gctgctggaa gtagccgggc tggccgcctg cgccaacgaa atcccgcagg
    14881 ctgctgccgc ccgcggcgag ggcgtccgtc agggtctcgc ggatggcgtt gacgaggcgg
    14941 gcgcagcggc gtggcccgag gctgccggcg ggcgtcgtcg ggtcgatacc ggcgcggaac
    15001 aggctttccg atgcatagat gttgcccaca ccgacgacct gtcgcccgtc catcaggaac
    15061 agcttgacgg gcgtggcccg cccgcggctg gcgcggtgca tccaggtgcc gtcaaaggcc
    15121 tcggagaggg gctccagccc caggccggca agcagggggt gggcgtcgtc aggcccggtg
    15181 taccacagga ccagcccgaa gcggcggggg tcgcgcagac gcaggatgcg ctcgccgaag
    15241 cacagatcca cgtgatcgtg tttcccgggc ggctgctcga gggaaagtac gcgcagactg
    15301 ccggacatgc ccagatggat cagcacggtg ccggaggtga agcgcaagag caggtatttg
    15361 gcccggcgct ccaccgcgag gaggggctgg gcggtgatgc aagcgtccag gtcctccggt
    15421 accggcaggc gcagacggcc gttgcggatg agcgcgccgg tggcggtccg gccggtgagc
    15481 tcaggagcga tgccgcggcg cgtggtctcg acttcgggca gttcgggcat ggcggatgca
    15541 atggagaggc gggcaagggg gccggcttgc tggggggctg gaaagcgcct aacatccggc
    15601 gaacccaatt gtagcggcgt gatccaaccc gttgtccacc cacgccgggg ccttgcgggt
    15661 cggggccacg cccaccgcag gcgctccgcc agtcagtccg agtcatgccg atgagccccc
    15721 gtccttctgc gttccctgtc cgcgtcctgg tggccgcggc gtgctttgcc acagccgtgc
    15781 cggccgccgc tcaactgggg ggcgacgacg cggagaccag ttcggccgcg gccctgccgg
    15841 gtcaggtgct gacgccccag atcttctacc aggtcatgct cgcagaggtg gcggctgccc
    15901 gcggtcaggg cgctgccggg gcccaggctt acctggacct cgcccggcgt acccgcgacc
    15961 cacgcctggc gcggcgggcc gccgagctgg ctgccctcag ccgcaaggcc gacctggcgg
    16021 gcgaagccgc ccagctctgg ctggagctcg accccgcgtc gccccaggcc caacaggtgg
    16081 cggcaggctt catggcatcc agtgccgccc ggatcgacgc gctgcgcagt caactggcca
    16141 aggctctcgc cactcagggc gatcagaccg ggccggtgct actgtccctc aatcgcggtc
    16201 tggcccggat tcccgacaag gccttggtgc gcacgctggt cttcgagctc accgaacctt
    16261 atctgctgct gccggaggct cattttgccc gttccaatgc cgcgtacgtg gccggcgacg
    16321 cgcggggcgc gttgacggcc atcgacagtg ccctggcgat ccgcccggac tgggagcagg
    16381 ccgtgcttct caaggcccag ctgctccagg aggtggtcgc gccggcgcag gggcagacct
    16441 atctgaagga ttatctggcg gcccatccgg atgggcggga ggcgcgcctg acgctggccc
    16501 gcatgcttgt tgccgatcgg cagttcggag ccgcgctgga gcagttcaga gcgttgctgc
    16561 aacgcctgcc ggacgaccgg gacgtggttc acgcgactgc gctgctgtcc atgcagcagg
    16621 gcgacacagc cagcgcagaa aagctcttcc ggcatctcct cgatctgggt ttcggcgacg
    16681 cggaccagat ccgcgcctat ctcgggcaga tcgccgagga cggcaagcgg cccgacgaag
    16741 cgctcggctg gtacaggtcc gtgaagccgg gaacccagta cctgccggcc cagacgcaca
    16801 tcgcgcagat cctcgcagcc cagggcaggc tcggcgaggc gcgtcagcat ctgcaccagg
    16861 gtgcccgcga gaaccctgcc gaccgcgttg cctacctcct caccgaggcc cagatcctgc
    16921 gcgatgcggg gcgcaacgcc gaatccttcg aattgctcga cgatgccctg cggacccgtc
    16981 cggacgaccc ggacctgctc tatgaatcgg ccctgatggc cgaacgcctc ggtcacatcg
    17041 atgtgatgga aggccgcctg cgcaagctta ttgccctcaa gcccgagcac gcccacgcct
    17101 acaacgccct gggctattcc ctggcggacc ggggcctgcg tctggatgag gccgagacgc
    17161 tgattgcccg tgggctcgca ttgtcgccgg aggacccgtt catcctcgac agcctggggt
    17221 gggtgcgcta ccgcaagggc gatctggcgg gggcgctggc ggcgctgcag aaggcctacg
    17281 gcgtgcgtgc cgatccggag atcgcggcgc atctcggcga agtgctgtgg acgctcgggc
    17341 gtcgcgacga ggcggcccag acgtggcgtg atgcgctgaa ggcccatccg gacaacaccg
    17401 tgctgtcgga cgtgctgaag aagttcaaac cttgagacgg gcctgtcatg tcctgatcgc
    17461 gaccgcggtg ctcgcgctgg ccggctgcgc cggcgtgccg tctgggccgc cggtgggcgt
    17521 tcccggcgtg cgtccggcag agcccttgca gcgattcgcc ctcgaagggc gcctgcaggt
    17581 gaaggatggt gagcgtctgg cggcggtggg tgtgtcgtgg cgccatgagc gcgggcacga
    17641 cgaatggttg tttaccggcc cgctcggtca ggggcttgcc cggctggagt ccggccccga
    17701 gggggcacgt ctgagtttcg ccgacggccg tcatcaggac gccgcagcgg caacggaact
    17761 cgctgccgcg gtgctcggtg tcgaggcccc gttcgaagcc ttgcccgcgt gggtcacggc
    17821 ccgtttgcgc gagggggcgg aggcgagggc ggtggacgcc gtcgcgcgtc cgacacgtgt
    17881 ggtggatgcc gggtggaccg tcgagtatgt cgattatgtc ggcccggagc ccgatgcacg
    17941 gccccgccgc atcgacatcc atcggggcga cacgcggctg cgcctcgtgg tggatgactg
    18001 gaacccctga gatgaatccg accgatttcg atccatccat gtccgtctgc gtaccggcgc
    18061 cggccaagat caacctgttc ctgcacatcg tgggccgtcg tgccgacggt tatcatctcc
    18121 tgcagaccgc cttccgcatg ctcgactggg gcgacgagat cacgctgaag cgccgcgacg
    18181 acggtgtcat tcgacggacc gccgatgtgc ccggcgtgcc ggctgagcgc gatctggtgg
    18241 tgcgggccgc ccaggccctg caggcggcaa gcggcactcg ctacggtgcg gacatcggcg
    18301 tgagcaagcg cattcccatg gggggcgggc tgggcggtgg cagctcggat gccgccagcg
    18361 tgctgctggc cctcaaccgg ctgtggggct gcggcctgtc acgaacgcgc ctgcaggaga
    18421 tcggcctgac cctcggcgcg gacgtgccct tcttcatttt tggcgagacg gcgttcgcgg
    18481 aaggcgtggg cgaagcgctg gtgccgctgg cggtgccgcc ggcgtggtac gtgatcgtgg
    18541 cgccgccggt cgaagtgccg accgcgaaga ttttttcggc gccgggcttg acgcgcgata
    18601 gcgaactcat caaaatgcgc gacttcgcaa cggacaccac gcgaaacgac atgcaggcaa
    18661 cggtctgcgc gatgtttccg gaggtcgccg aggcgctggt ctggttgtcg tcccgagggg
    18721 cggcgcggat gagtggttcg ggagcctgtt tgtttgccgc cttcagcagc aaggcacaag
    18781 cagaacaggt ggccgccgcg gcaccgcagg gtctgaaggt ctgggtcgct cccgggctag
    18841 acaggcatcc cctgtgggct tgggtggggt gaagtgtgaa agcggtttct tggggagtcg
    18901 ccaagtcggt taaggcaccg gattttgatt ccggcattcg agggttcgaa tccttcctcc
    18961 ccagccaaac gacagcgggc aggccgaaag cctgcccgtt tgtgttttag cggttcaacg
    19021 atgcagcaac acgagaggtg atcatggctt cgggcagcct gatggtattc accggcaacg
    19081 ccaaccccaa gctggccgcg gatgtggtca ggcgcctggg acggtctctg gggtcggcca
    19141 cggtgggccg tttctccgac ggtgaagtca atgtggaact gctggagaat gtgcgtggca
    19201 aggacgtctt cgtcctgcaa cccaccggcg ctcccacgaa tgacaatatc atggagctga
    19261 tgatcatggt ggacgcgctg aagcgcgcct ccgccggccg catcaccgcc gcaatcccct
    19321 attttggtta tgcccggcag gaccgccggc cgcgctcggc gcgggtgccc atcaccgcca
    19381 aggtggtggc caacatgctg caggccgtgg gcgtgcagcg tgtcctcacc gtggatctcc
    19441 atgccgacca gatccagggc ttcttcgaca ttcccgtcga caacatctac gccgcaccgg
    19501 tcctcctcga cgatctcgag aagcagaagt acgacgactt gttggtcgtg tcgccggacg
    19561 tgggtggcgt ggtgcgggcg cgggcctttg ccaagcgcct ggaatgcgat ctggcgatca
    19621 tcgacaagcg tcgtcccaag gccaatgtgt ccgaggtgat gaacatcatc ggtgaggtcg
    19681 acgggcgtac ctgcgtgatc atggacgaca tcgtcgacac cgccggcacg ctgtgcaagg
    19741 cggctcaggc cctcaaggag aacggtgcca agcgggtgat ggcctactgc acccacgccg
    19801 tgctgtccgg ccccgccgtg tcgcggatca tggattccga cctggacgaa ctggtggtca
    19861 ccgataccat tccgctgacc gacgaagcgc gcgcctgttc gcgcatccgc acggtctcca
    19921 tcgcctccct gctggccgac accatcgtgc gcatcagcaa cgaggaatcg gtgagttccc
    19981 tgttcaccga gtgaattttt tcggctggcg gaccccgttt gccggccatc accccagccc
    20041 gtctggtcgc ggacgggccc atcaacctgg agcaacaagc atgaccattc aattcaatgc
    20101 ctccaagcgt gaagcgcagg gttcgagtgc gagccgccgc ctgcgtcgcg ccggccgcgt
    20161 gcccgccatc atctacggtg gcgaagtcgc cccgctgcaa gtggaactgg atcacaacga
    20221 gatcttccac ctgctgcgca acgaagcctt ccactcctcc gtgctcaccg ccctggtgga
    20281 cggggctgcc caggccgtga tcctgcggga cacccagtgg cacccgtaca agcagcaagt
    20341 gctgcacctg gacttccagc gcgtcgattc cactcacaag atccacgtga aggtgcccct
    20401 gcacttcgtg aacgccgaca tcgccccggg cgtcaagctc gaaggcggca tcgtgtctca
    20461 cgtgatgacc gaactggatc tggaatgcct gcccggcgat ctgcccgagt tcgtcgaggt
    20521 ggacctgaag gatctcaagg gtggcgcgtc catccacgtt tcgcacctgc ccctgccggc
    20581 cggtgtgaag gccctgaccc atggtgaaga cccggtcgtg gccaccatcc tggcggtccg
    20641 tggcggtggc gaggacgaag ccgctgccga aaccccggcg gcctgatccg caaccggatg
    20701 accggggccg tcctgcgatg agccagactg cgccccggct tgtcgtcggc ctggggaatc
    20761 ccggcgccga atattccgaa acccggcata acgccgggtt ttggttttgt gagcggctgg
    20821 cgcgggaact gggcgcctcc ttttcgaagg aagcccgctt ccatggcctg gccgcccacg
    20881 cccgcgacgc cggcgtatgg ctgctcatgc cccagacctt catgaaccgc tccggtcagg
    20941 ccgtggcggc gctggcacgc ttctaccgga taccgccggc cgagatcctg gtggtccacg
    21001 acgagctgga catcccgccg gcgcacctgc gcctcaagtt cggcggcggc ctcggcggcc
    21061 ataacggcct caaggacatc accgcccacc tgggcaccca ggactactgg cgcctgcgca
    21121 tcggcatcgg acatccaggc gatcgcaacg aagtggtgaa ctttgtcctc aagccgccgc
    21181 ggcgcgagga ggcggccctc atcgacgatg ccctcgaccg ggcgctggcc gtctggcccc
    21241 gtattgccgc gggcgactgg aacgccgcga cgcagcgcat caatgcccgt ccgccgggtg
    21301 atgcgaagaa gtaagcgaaa cctgtttttt catcaattca gtcaataatc tcgaaaattc
    21361 cgccaaaaag ctaaagcatc ccccgcgtgc gccgtagtcc agtcccgata cagcgactca
    21421 ccaaacgggg cagcgagttc ccgtccttga ggggatatgg agatgcgaag gcttgcggat
    21481 acgcccatct ggctgagatt gacggcggcc atctggctga tgctggtgat cgcctggggc
    21541 ggcatgatcg tgtgggagac acgcaccaac cgggagaccg ccgtcgccca ggccgaggat
    21601 ttcgcgcgca gcatccacga gatgaccatg gcaggtctga ccggcatgat gatcaccggc
    21661 accgtcgggc agcgcgaagt ctttcttgat cagatcaagc agctgtcgat catccatgac
    21721 ctgcaggtcc tgcggggcga cgccgtgagc cgccagttcg ggcccggcac gcgcccccag
    21781 ccgcccctgg atgccgatga gcaggcggtg ctggcgacgg ggcaggcggt cagccgaatt
    21841 cagaccgatc cgcgtcttgg tgaatatcta cgggtgatcc gccctgccat tgcgtccgac
    21901 aactatcttg gcaagaactg cattggctgc caccttgtcc cggcgggtac gcccctggga
    21961 ctggtcagca tgaaggtatc gctggacaag gtgaacgagg ccgtggagtc cttccgctgg
    22021 aaaagcatca tcagcgcgct ggtgctgtcc cttccgctga tcctgttcgt cacctacttc
    22081 atccgcagct tcgtggcccg ccccctggcg gacatgaacc gcagtctgca tgagatcgcc
    22141 aagggggagg gcgatctgac gcgccgcctg ccggtacgcg gtcaggacga gatcgggcag
    22201 gccgccgacg ccttcaatgc catgctcggc acggtcgcca gtctggtgcg gcgggtgtcc
    22261 gattccgcgt cgcgggtgac cgcgtcgtcc catcagctga cgatgagcgc gagtcgcgtg
    22321 gcggagggtg cgcgcgccca gtgcgagcag tccgccagcg cggccagcga ggtggagacc
    22381 atggcctcgg atatcgccca tgtggcggtc agcaccgaag acgtgcacca gcgatcccgc
    22441 gagagtctgc gccgttccca ggacggcagt cgaagtctgg acatgctggt gggtgaagtc
    22501 ggacaggcgg aagcctcggt acgccagatg gccgactcgg tcggtcagtt cgtcgaatcc
    22561 accagcgcca tcactaccat gactcaggaa gtgcgggaga tcgcggagca gaccaatctg
    22621 ctcgccctga acgccgcgat cgaggccgcg cgggccggcg aacaggggcg ggggttcgct
    22681 gtggtggccg acgaggtacg caagctggcc gagaagtcgg ccaggtcggc gggtgagatc
    22741 gacgcggtca cctcgcgcct gtctcagcag tcggtggccg tgcgtcaagc catccacgaa
    22801 gggctcgagc atctggaaag cagccgggtg gccgtcggcg aggtatcagc cgtgatcgtt
    22861 tcggccaacg agtcggtcga agccgtaggc cgcggtctcg acgagatcgc cggcgccacc
    22921 gagcgtcagc gcgcggcgag ccgtcgtgtg gcggaaagca tcgatgccat ggcagagatg
    22981 gcccgggcca gcaatggggc agtggaatcc acagccgacg ccgcccgcga tctcgagcgg
    23041 ctggccagtg agctgcagga gacggtggcg cgcttccggg tctgaggcag cggccggggg
    23101 cggctatact cgatcccctg tgtccttctc cgggtgagcg tcatgcagca cttcatcgcc
    23161 gacgatggcg aatgtctcca cgtccaggtg tcgggcgagg ggccgccggt catcctcctg
    23221 cacggttgga cgtcgtccca ccgggactgg aatcccttcg tggatgtctt gtcggccgat
    23281 caccacgtgc tgcgctggga tgcccgcggc cacgccaccc atgcgctcca ggccgccacc
    23341 gcgcccaccg tgcaacgcat ggcgcgcgat ctggcgaatc tgctggatca ctggcacctg
    23401 gaacgggcgg tgctggtcgg tcattccatg ggcgcgctga ccctgtggca atacctccag
    23461 gactttggca gcgaccgcgt cgccggggtg gtgatcgtgg accagtcgcc ccggctggtg
    23521 acggatgcca cctggcggaa cggcatctac ggcgatttcg ataccgcccg caacgcggcc
    23581 tttatcgcgg aactggaggc ggattttggc gaagcggtgc tgcgcctgac cgccttcggt
    23641 cgcaatggcg aggtgcgccg gcgttacacc gaccagcccg agagcttcga caacgcccgg
    23701 cgctggctcg ggcgtctgca tcacgctgcg ctcattgcgt gctggcggac ccttgccgcg
    23761 gcggactacc gggcggtgct gccggccatc gatgtaccgg ccttgctggt gtatggcgcc
    23821 gagagcaatt tctacagccg ggagacggcc gaatgggtgg ccggcgcgat ccccgatgcg
    23881 cgcctgacgg tgtatcccgg cgccgaccat tcgccccacc tgcacgaccg ggcgcgtttc
    23941 ggggcggatc tggacgactt tctggcgcgg gtctttccgc gctgaggggg cgggggacgc
    24001 cgcgcgttgc ggcgtccata ggggcgcggc tcagcggatg ccggccttgt ggatcagagc
    24061 ctggatctcg ccgaccagac cgcggcggaa ggcgagcacg cagagcacga agatcgcccc
    24121 catgatcacc gtcacccacg atcccacctt gtcggccagc tcgttctgca gggtgacgat
    24181 cacgcccgag ccgacggacg ggccaaggat ggtgccgaga ccgcccagca gggtcatcag
    24241 gaccacctcg ccggacatgt gccaatgcac atccgacagg gacacgagct ggaacaccag
    24301 ggtcttggtg gcacccgcca gtccggccag ggaggccgag atcacgaaag cgatgagctt
    24361 gaagcggctt acgtcgtagc ccagggagat ggcgcgcggc tcgttctcgc ggatcgcctt
    24421 gaggatctgg ccgaagggcg agtggatggt gcggtggatg atgaagaagc cgagggcgaa
    24481 caccccgtac accaggtaat acatggccag gttgtcggtg agatcgatga agccgaacag
    24541 gtggccgcgg ggcacgccct gcaggccgtc ctcgccgccg gtggccttca cctgcaggac
    24601 gatgaaatac accatctggg cgagggccag ggtgatcatc gcgaagtaga tgccggtgcg
    24661 gcggatcgcc agcaccccga acagccagcc cagcaggccg gcggccgcag tgccggccag
    24721 gatgccgacc tccggcgtga cgcccacgtc gcgcaccatc atcccgcaca cgtagcccgc
    24781 gctgccaagg aaggccgcat ggccgaagga cagcaggccg gcaaatccga gcagcaggtt
    24841 gaaggcgctg gcaaagagtg cgaagcagaa cagtttcatc aggaaggtgg gatacaccgc
    24901 gaacggggcg atcaggccca cgatgaagag cgcggtgtac aacacctcgg gtttgatcag
    24961 ggcggtgggt tggcgggacg cgttcattga attcctcgcg atcgggcggg ctcaggcctt
    25021 gccgaacagg cccgccggac ggaccagcag gacgatgacc atgatgacga agacgaccac
    25081 cgccgagcct tccgggtaga acacccgggt gaggccttcg atgaggccca ggccgatgcc
    25141 ggtgaggatc gagcccatga tggagcccat gccgccgatc accaccacgg caaagacgac
    25201 gatgatcagg ttggagccca tcagcggatt gacctggaag atcggcgccg cgagcacgcc
    25261 ggcgaaggct gccagagcca ccccgtagcc gtaggtgaag gtgatcagca gcggcacgtt
    25321 gataccgagc gcctggacga tctgcggatt ctcggtgccg gcacgcaggt aggcgccgag
    25381 cttggtcttc tcgatcatga accaggtgcc gaagcacacg gtcagggacg cgacgatcac
    25441 ccaggcgcgg tagatcggca ggaacatgaa gcccaggttg atgccacccg cgagggcttc
    25501 cggcacgtcg taggattcgc cggagatgcc gaactggtcg cggaacacgc cttcgatgat
    25561 gagggccagg ccaaaggtga ggagcaggcc gtagaggtgg tccagcttgt agagtttgcg
    25621 cagcatggtg cgctcgagca ccacgccgaa agcgcccacc agcagcggcg cggcgatcag
    25681 catcacccag tagttgatct cgaagcgggt aagggcgatc cacgcgacga aggcgccgag
    25741 catgtactgg gcgccgtggg agaaattgat gatgttgagc aggccgaaga tgatcgccag
    25801 cccgaggctg aggatcgcgt agaaggagcc gttgatcagc cccaccagca actggctgcc
    25861 cagcagggcg gtcggcacgc cgaagatttc catgggtcac tccgggcccg ggccggcgca
    25921 ggccgatccg ggcgctgtgg cggttgcgat tacttcttga ccagcgggca gcgggacagg
    25981 gacaggggct ggaaggcttc gtcggcagga atcacctgac ggacgttgta gtagtcccac
    26041 ggatacttgg actcagcagg cttcttcacc tgcatcaggt acatgtcgtg gaccatccgt
    26101 ccgtcttcgc ggatgcgacc gttcttggcg aagaaatcgt tgatgggcgt cttcttcatc
    26161 atttccatga ccttcttggt gtcgtcggta ccggcggcct tgacggcctt caggtagtga
    26221 tacaccgaag agtactggcc ggcctggacc atggtgggca tcttcttctg cgcacccatg
    26281 aagcggcgcg accattcgcg ggtctgatcg ttgaggtccc agtagaaacc ttcggtcagg
    26341 tacatgccct gggtggcctt gaggccgagg gcatggatgt cggtgatgaa catcagcagt
    26401 ccggccaggg actgcttggg cgtgatgccg aactctgcgg cctgcttgat ggagttggtc
    26461 gtgtcggcac ccgcattggc caggccgatc acctgggcgc cggaggcctg ggccttgagc
    26521 aggaaggagg agaaatccga acccgggaag gggtgacgca ccgaaccgag cacctggccg
    26581 ccgttggcgg tgaccaccgc ggacgcatcc ttctcgaggg cctgaccgaa ggcatagtcg
    26641 gcggtgagga agaaccaggt cttgcccccc tgcttggtga cggccttggc ggtgccgttg
    26701 gccagggcgt aggtgtcgta ggtgtaatgg acggtgacgt cgttgcactg ttcgttggtg
    26761 atgggggtgg aggcggggcc gctcatcagg gcgatgcgat ccttttcctt ggcgatcttc
    26821 atgaccgcca gggcgacgga ggtggtgacg agttcggagg ccacgtccac gccttcgcgc
    26881 tcgaaccatt cccgggcctt gttggaggcg atgtcggcct tgttctgatg gtcggcgctg
    26941 acgacctcga tcttgaacgt gggcttttcc ttggcgatga aatcttcgac cgccatcttg
    27001 gtggcgagta cggcgcccga tcccgccagg tcggagtagg tgccggacag gtcggtgagg
    27061 acgccgatct tgaccacgcc gccggaaatc tgcgcatggg cgccggcaga cagggtggca
    27121 agggcggcag tgcaggcggc ggtcagcagc ttcttcgagg tcgtcatgtc tctctccttg
    27181 ttgtgtaatg ggggggtggg cgtcacacgc ccagggtttg atgcagccag tccatccgcc
    27241 cgggcagttc ctcctgggga atggtggcga ggatctcgcc gtgttcgagc acgtagtggc
    27301 ggtccgccag cggggctgcg aagcggaagt tctgttccac caggatgatg gtgaagccgc
    27361 gtgatttgag ttcgacgatg acttctccga gcttcttgac gatgaccggc gccaggccct
    27421 cggtgatttc gtccagcagc aggagtttgg cgccggtgcg cagcacgcgg gccatggcga
    27481 gcatctgctg ttcgccacca gagagcttgg tgccggggct gctgcggcgc tcccgcaggt
    27541 tggggaacat tgcgtagatc tcgtcaaccg tcatgccacc gctcgccacc tgggggggca
    27601 gcaacaggtt ctcctcggcg ctgagggagg cgaagatggc ccgctcctcg gggacgtagc
    27661 ccaccccgta gccggcgatg cggtgggtcg gctcggcgac gatttccttg ccgttaagca
    27721 tgatggagcc ggtgcgccga cccaccaggc cgaggagggc cttgagggtg gtggtgcgtc
    27781 cggcgccgtt gcgacccagc agggtgacgc attcgccgcg tccgacggag aagtcgatgc
    27841 cgtgcaggat gtgggattcg ccgtagtagg cgtgcaggtc gcgcacgcgc agcatctcgg
    27901 gtttgggatc gaaggcgctg gtcatgacgc gtcgggtctc agtggtgggc gtcgttcatg
    27961 tcgctggagc ccacataggc ttccagcacc tggggattct tcgagacgtc ctcgtaaggc
    28021 ccctcggcga ggatgctgcc ccgttgcagg acggtgatgc gatcgcacag gtgggacacc
    28081 acggagaggt tgtgctccac catcaggatc gtgcggtcga ccgagacctt gcggatcagc
    28141 tcgacgatgc ggccgatgtc ctcgtggccc atgccctggg tcggttcgtc gaggagcatc
    28201 atcgcgggct ccagtgccag ggtggtggcg atctccaggg cgcgcttgcg gccgtagggc
    28261 atctcggcgg tgacggtgtc ggcgaagcgg gtcagatcga cggcctcgag gagctccatg
    28321 gcccggtcgt tgaggacatc caggctcttt tccgagcgcc agaaatgaaa ctcggtgccc
    28381 agcttgcgct gcaggccgat gcgcacgttc tccatcacgg tgaggtgcgg gaaggtggcg
    28441 gagatctgga aggagcgtac aaggcctcgg cgggcggtga cggcgggggc ctcgcgggtg
    28501 atgtcgcgcc cgtcgaagtg aatggtgccg cgggtgggag gcaggaactt ggtgaggagg
    28561 ttaaagaccg tggtcttgcc cgcaccgttg gggccgatga gggcgtgaat gtggccgcga
    28621 cggactttga ggtccacgtt cgagacggcc gtgaatccct tgaattcctt ggtcaggcca
    28681 gctgtttcca ggatgtagtc gctcatgacg ggctcagcgg cgagtgcagc ggggctgcag
    28741 gacggacccg atacgtcgtg ggcgcagggg gcgggggtgc cggggcagga cgaggctggc
    28801 ggcaagcagc atccagcaac aggcgaaagc ggtcatgaag gcgggtctcc gtgttgcgcc
    28861 gcgggcgctg tgggcgcgct gcgattttgt ttatgtgtct attaagacgc ttaattgctg
    28921 tggtggccat gagggaatac ccgaatgtcc gtggccgtca acgcaccgca aaggtgcgtc
    28981 gctgcgtgat gtggcggatc gatcccggat cggggctaga atggaggatt gacagtcttc
    29041 ccgtcagtga tcacggagtt tcccgaaatg ccccagtatc gttcccgcac ctccaccgcc
    29101 ggtcgcaaca tggccggcgc ccgcgccctg tggcgcgcca ccggcatgaa ggacggcg
//
LOCUS       DAOQPA010000034        29102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_725_length_29102_cov_17.155231, whole genome shotgun sequence.
ACCESSION   DAOQPA010000034 DAOQPA010000000
VERSION     DAOQPA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 29102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29102
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_725_length_29102_cov_17.155231"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /gene="ribH"
                     /locus_tag="PKX01_06810"
     CDS             complement(<1..437)
                     /gene="ribH"
                     /locus_tag="PKX01_06810"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMZ75872.1"
                     /translation="MPRYDNIPEIAPSLDGQGLRIGIVMCRFNLDVCEGLLSACTAEL
                     LRLGVAPDAILIATVPGALEAPLTLQTMAKSGRYDALVALGAVIRGETYHFELVSNEQ
                     GAGITRVTLDTGVPIANGILTTEDDDQALARMQVKGADCAQAAV"
     gene            complement(474..1568)
                     /gene="ribBA"
                     /locus_tag="PKX01_06815"
     CDS             complement(474..1568)
                     /gene="ribBA"
                     /locus_tag="PKX01_06815"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HMZ75873.1"
                     /translation="MSALSPIQDIIADIKAGRMVVLVDEEDRENEGDIVLAAEHVTPD
                     AINFMAKHARGLICLTLTEARCRQLGINQMARDNRSQFSTAFTVSIEAAEGVTTGISA
                     ADRARTVQAAVARHAKPCDIVQPGHIFPIMARPGGVLIRAGHTEAGCDLAAIAGLEPA
                     SVICEIMNDDGTMARLPQLIDFAQTHGLRIGAIRDLIEYRARTEKLVTRVSEKTVDTP
                     HGAFRLCAFEDTTSGEVHLAMVHGEIRPDVETLARVHEPVSVLDFLDPAAGRHSFTVD
                     DALRRIAAHGNGVLVLLYRPQSGQDLLDQLGTTVPRPAQKWDPRLFGIGAQILRELHV
                     GKMKLMASPRKIPSMAGFGLTLSGHVTPDA"
     gene            complement(1565..2176)
                     /locus_tag="PKX01_06820"
     CDS             complement(1565..2176)
                     /locus_tag="PKX01_06820"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMZ75874.1"
                     /translation="MFSGIVAATGRILDVAPLEDGVRLTVDVGALGLDDVQLGDSIAH
                     NGVCLTVIAREGNRVQFDVSRETLNCTVGLDAPGEVNLEKALRLADRLGGHLVTGHVD
                     GIGEVVKFAPIGESHELVIRAPAALAGYIARKGSITVDGVSLTVNWVKGRDFSINLIP
                     HTVQVTTLKRLAPGARVNLEIDLIARYVERMQAWRESGEEATS"
     gene            complement(2329..2760)
                     /locus_tag="PKX01_06825"
     CDS             complement(2329..2760)
                     /locus_tag="PKX01_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ75875.1"
                     /translation="MHRKLREVVAGQTVVSASASTTVRAAAVTMAQKNIGAIVVVDAA
                     GKLEGLFTERDVLNRVVARSLDPDTTPLSAVMTAKLQTASPDQPLAHALHMMFEGGFR
                     HVPVVEGGLPIGMVSARNALGPEMVEFEKELQDRDHITEIL"
     gene            complement(2851..2985)
                     /locus_tag="PKX01_06830"
     CDS             complement(2851..2985)
                     /locus_tag="PKX01_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75876.1"
                     /translation="MTRTIALLLALALGFATTAQACGDHATKNSKPDMSKPAGPKTGT
                     "
     gene            complement(3214..4167)
                     /locus_tag="PKX01_06835"
     CDS             complement(3214..4167)
                     /locus_tag="PKX01_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ75877.1"
                     /translation="MSTGNAPLQPIAFPAADGYILRGFAGCHSQAAATRPVVIINPAT
                     SVRCRYYARFATWLHGHGFDVITYDYRGIGESRPPQLRGFEAGWIDWGRLDFEGALRF
                     AAARFPGQPVHVAAHSVGGFLIGLAPSSPCVARIFTMGAQYAYWRDYARPRRAGLLLK
                     WHVAMPVLTAVFGYFPGRRLGWLEDTPRGVVRDWTARQPRFEDSWRSGARVLPEAERR
                     ALVERFAAVRAATLAVSLSDDEFGTVEAIRRLLGYYRRSDRTHLHLTPEAVGQRTVGH
                     FAFFHSRFEHSLWPIALQWLRHGHLAARPIGEVWHLAADGG"
     gene            complement(4287..7502)
                     /locus_tag="PKX01_06840"
     CDS             complement(4287..7502)
                     /locus_tag="PKX01_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ75878.1"
                     /translation="MRPPSRLPSFSAQISRSFALLFTLAICAILAGWYFGVPGLGLQG
                     ERQRYIGTSIQLLEQGADFQRARLVSRLRAHRGNLLTLAENRVITGHLMARRDDDAAR
                     DIERVLERLMRAYPDDYRAVQVISAESGTLLGAVGKGQTGQAFAHPELLDTLQEPGIL
                     ELIRLLPVRGGGRDELVIARQMLAPGPDGTPGTQLVGYLVAFLNQDLLLFEDSGDDTA
                     PDEARRQTLLADTGGAVLVNTLGPGTDTTIRPLLGVSTGFEGTLRLTVAGLDEVVASY
                     RHVPINGTQGLRLVHFQRQEDMLGGVKEVTDTLVALGGAFLVLGLILVWDAARRLTRP
                     LGELLRVVDAVGAGQPAPPFEPDRADCRELAHLGTAFNEMAERVAETRLDLENRVAER
                     TAELARERSFLETVLKTVPALIWLKAPDGRYLACNAEFERFFGATEGDILGRTDYDFV
                     PRELADFFREHDRRATERGGPSVNEEWVTYASDGRRVLLETTKTPLRGPDGSLLGVLG
                     IGFDITARKQAEGALLDNQRTLMAKVDERTRDLAHAKDAAEAANRAKSTFLANMSHEI
                     RTPLNAIIGLTHLLRRDQPAPRQAERLDKIDASGRHLLAIINDILDLSKIEAGKLTLD
                     DQDFALGQILDHVASLVGESAASKGLRIVIDEDHVPRWLRGDVTRVRQALLNYASNAV
                     KFSRHGTIVLRAELLQDAGDTMHVRFAVEDQGDGIPLDKQANLFHEFEQADTSTTREH
                     GGTGLGLAITRRLAGLMGGEAGFESTPGQGSRFWFTAWLRRGRGVMPPPEAPARDTAA
                     SAEHLLRARHAGARLLLAEDNRINVEVALELLHGVSLHADVAENGRVAVEMAHGNTYD
                     LILMDMQMPEMDGLEACRQIRALPGWAARPILAMTANAFDDDRAACQAAGMNDFIAKP
                     VDPAALYATLLRWLPAAAGPIDASTATPPSVEPAPTPAAAPEDRLATAQGVDLARGLR
                     ITRNNRERYLHLLEGALDGLQQHLLTLRERLAAGDFSGAKPIAHTVKGTAGNVGLTTV
                     AAVAIELDEVLRGPDPEANRCSQLMDALDAALAEVAHALHD"
     gene            complement(7510..8571)
                     /locus_tag="PKX01_06845"
     CDS             complement(7510..8571)
                     /locus_tag="PKX01_06845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ75879.1"
                     /translation="MRPRLLLGLLVLSIAMPALAERDTPPVGLSPASPEVDVGIQPLG
                     VPSGVISAVMSRDRILRQALESLGTPPRFHAFWRGADIVPLLADRRLEAGLLGDMPTL
                     MACARGQAVIVGLVKQTSTAIVGRRVAQVSDLAGRRIGYVEGSSAHHTLLQGLRSAGL
                     SERQVRLVPLAVSEMPEALERGEIDAFAAWEPAPSAALAANRANRIVFRGLTSDFLVL
                     DRRFAEQQPAAARHLIAGFVRALEWMRRAQANLDRAVSWARLDAQTLSGKAPPISDRQ
                     IARITHEEILDVPSAPAIPGRSTSPPLRKEFDFLAGQGKLPPEARWDAIADALRYDGL
                     YQVLAEPRRFRLAEFDYAD"
     gene            8844..9623
                     /locus_tag="PKX01_06850"
     CDS             8844..9623
                     /locus_tag="PKX01_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75880.1"
                     /translation="MDSLSAALQRLFGRPGQPLPDPWPESGALLEPVDPDGRAWALVI
                     GLPRPAGGAMVRALCEGATALDLPVPAVSLAGDAGFHVWFALTDPVPAERGRVFLEAL
                     CRRCLPEGASRALRLPGTVGLTGLSLPPARDGGRWTAFIDPSLAGMFDGETWLDMAPS
                     PERQAELLAGFRAIRPAQFDRALAELDPPGAVVPSPRGALAGQVVEPPALSGTVPPLG
                     LGVGSGFVDPRTFLLAVMNDPGASADHRIDAAKALLPYFER"
     gene            complement(9634..10143)
                     /locus_tag="PKX01_06855"
     CDS             complement(9634..10143)
                     /locus_tag="PKX01_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HMZ75881.1"
                     /translation="MPAFLHRLRGHAAPLPPAPPLRAILLAGLGGFLAIAVVAGLAER
                     TAAALVLGSFGASCVLVFGFPEVPFSQPRNVVLGHVLSSAVGLACLSLAGPHWWAAAL
                     AVALAIMGMMALRCVHPPAGSNPVIVFLLQPGWSFLLLPTLAGALLLVGVALVYNNLA
                     RDADWPRYW"
     gene            10369..10974
                     /locus_tag="PKX01_06860"
     CDS             10369..10974
                     /locus_tag="PKX01_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ75882.1"
                     /translation="MTAAPPSPAESAAPMHSRSERRAHLVDTALRLFARGGYHATGID
                     AVLAASGVAKKTLYHHFPSKDDLIVEVLRERDRRFRASLAAFVAARATPRARLAAVFE
                     WHALWFAEPDFAGCMFLNAAAEFHDGSAGILAVAREHKQALEAWLAALLTDLVEAERA
                     APLAADLNLLLDGAIGTALVSRDPAAAARAWALAERLLPAA"
     gene            10985..11740
                     /locus_tag="PKX01_06865"
     CDS             10985..11740
                     /locus_tag="PKX01_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75883.1"
                     /translation="MRHAVFVLLLLASAWPARASDEARLPGDAAAVRAADLEKPFKLS
                     LGEYRYGGNFSGQDVNLRYRRGDSSAWLGSYRDRDFGTQTRAGADTSWQPFAGSTLAL
                     QPSVQLASGGFVGGSFSVELGEPWFVQAGIGRTNTRPYFNLNFDPNDAQSYAVGYRDD
                     SGQTYYLMSVRDDRLGTRQRHTHAVARWPAGPTRLTVDVLHKTGDGDNGHVDAWGATL
                     GVDYLDWFVRLVRDPHQNFGDANATRLTVGRRF"
     gene            complement(11788..12555)
                     /locus_tag="PKX01_06870"
     CDS             complement(11788..12555)
                     /locus_tag="PKX01_06870"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HMZ75884.1"
                     /translation="MNRRINLNADLGESFGAWTMGSDAELLATVNSANIACGFHAGDP
                     LVMRTTLRHARAAGVGVGAHPSFPDLQGFGRRPMQLPPAELEAVLIYQMAALAGMAQA
                     EGVKMGHVKPHGALSNMTCTDAALARTVVAAIRAFDADLILLAPACSALLTAGAEAGL
                     RVVAEIFADRAYQDDGSLVPRSRPDAMIHGAGASLAHVSAMLAEQALISVNGVRRPTP
                     IGSVCVHGDGPDAVATARFLRDALTRDGWALVGLDAL"
     gene            complement(12641..13666)
                     /locus_tag="PKX01_06875"
     CDS             complement(12641..13666)
                     /locus_tag="PKX01_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HMZ75885.1"
                     /translation="MTHLHVLDGGLGVSVQDRGRVGYRALGVPVSGALDPLLLAAANA
                     LAGAPADAAALEILLHGPRLQAADGPVRVALAGAVASRVLRGDGSAEPLPPWRSATLT
                     PGDILAVGRVEAGVAVLAVSGGMTVPVVLGSRATYARAGLGGIAGRPIQAGDRLPCGA
                     APDGPERVGPPWRLPAGPLRVIAGPQRAHADEACFAAFLAGPWTVTPAADRMGARLAG
                     PRLTHSARGAEIVTDGVTPGAIQVPADGQPILLRADCQTTGGYPKIATVIAADLPRLG
                     HLRPGDTLSFSAVDAAAAARARQEQARLFAAWRAALSALPPAGGFDATALYAANLVGG
                     VLRGDED"
     gene            complement(13663..14439)
                     /gene="pxpB"
                     /locus_tag="PKX01_06880"
     CDS             complement(13663..14439)
                     /gene="pxpB"
                     /locus_tag="PKX01_06880"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HMZ75886.1"
                     /translation="MPRTAPAPMSATPNAPRLLPLGDTAFTVEFGTTIAPDLHARVRG
                     FAAGLAAAADAGALPGVIEWLPAFASVTVFFDPDNADPDALAAHLLSLARQTHAVPLN
                     GATWTLPVCFDEDFGPDLDDLAARCGLDRAGVIARLTGAEFSVWMLGFLPGFPYLGGL
                     PAELAQPRLATPRTAVPAGSLAVADALCAVYPWTSPGGWRLLGRCPVPCFDVRRSARP
                     ALLAPGDRVRWQAVTRSDFDTLAADFTAGRADPDRLRAAP"
     gene            complement(14675..15367)
                     /locus_tag="PKX01_06885"
     CDS             complement(14675..15367)
                     /locus_tag="PKX01_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ75887.1"
                     /translation="MRTLRGILKALLLLVICTQAWAFGLADLSDKDASGGLREALSQG
                     ASKAVGLLGTDGGFLNNPKVKIGLPESLAPVEGVLRTMGRGKDLDSLVATMNHAAEQA
                     VPKAKVLLVDAVKKMSVDDAKKILGGGDDAATQYFKEKTSAQLFESFLPTVKATTDKL
                     ALSQQYNALAGKAGSFGLGKGDDLKVENYVTRKALDGLFLMIAEEEKAIRKDPLGTAT
                     GLAKKAFGALGQ"
     gene            15398..15892
                     /locus_tag="PKX01_06890"
     CDS             15398..15892
                     /locus_tag="PKX01_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ75888.1"
                     /translation="MLPKAITRFIAALLFLALLDGCSAPAAPDVSFTTLDGQTTRLSA
                     LRGKVVLVNFWATTCTTCVGEMPKLVDTYRRFAPRGFETVAIAMSYDPPEYVRNYTAK
                     AGLPFKVALDARGEAARGFEEVRLTPTTFLLDKQGRVVQKYLGEPDFPKLHALLEKLL
                     ADPA"
     gene            15889..>16408
                     /locus_tag="PKX01_06895"
     CDS             15889..>16408
                     /locus_tag="PKX01_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HMZ75889.1"
                     /translation="MKRPVLSVALLAAAGLAALVLWPRGREVDGVAVRRAPLTQSVVA
                     TGRIATPARITVGAPLAATVLAISVREGDSVKPGQVLARLQADDADALVRQAAAALAE
                     AEARLSQIATVGEPVAAQALAQAEANLHVARREAERSRQLVAQGFYSQSRVDDALRIE
                     TNARNALDTTRAQ"
     assembly_gap    16409..16508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16509..17180
                     /locus_tag="PKX01_06900"
     CDS             <16509..17180
                     /locus_tag="PKX01_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ75890.1"
                     /translation="ARVDQARAQLANARARAALLTLTAPSAGTVLSRKAEPGDVTTAG
                     KVLLELAEAGETRVYATVDEKNLRLLALGQKARGVADAFPGTPFEAELYYLAPAVDPQ
                     RGTVEIRFRVPAPPAFLRPDMTISVETVTGHRDATLVLPSEALREADGGAPWVLVARD
                     GVAERVPVQTGLTGIGQVEILHGLVEGDVAILPAAGALDGDRVRLREMKKPRNTLQVP
                     GGMAR"
     gene            17704..18924
                     /locus_tag="PKX01_06905"
     CDS             17704..18924
                     /locus_tag="PKX01_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ75891.1"
                     /translation="MTLRLPFAPLVALRFLREGRMQTLLILAGTTGGVAVIVFLTQLI
                     TQLQAQIVDRVLGSQAHVVIRPLEEANRRAFAPDGMATVVEPRAQRLRSIDQWESVAR
                     LAAGLPGVTAVSPVATGPAFATRGQANKSIALMGVDADRYRRIVRMEEYMKAGRFAVD
                     GTRAVIGADLATDLGLRLEDKLRVMLPNGRDELLQVGGIFDIGNKDLNRRWVFVTLPL
                     AQSLLDLPGGVSNLDLTVADIFEARPIAERLRGATGLTVDSWMETNAQLLAALRNQTV
                     SNNLIRSFVVLIVALGIASVLVVSVVQKQKEIGILRAMGARRGMILQVFLIQGAVVGA
                     VGSVLGSALAFTLLRVFSSIYRNADGSPLFSPQLELRFIVMAALVATAVGLLSALIPA
                     RRAARMDPVQAIRA"
     gene            18921..19625
                     /locus_tag="PKX01_06910"
     CDS             18921..19625
                     /locus_tag="PKX01_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ75892.1"
                     /translation="MSALRPAAAGPVLELAGITKTYNPGTPVATEVLHGIDLSLAEGE
                     FTALVGPSGSGKSTLLNLIGLLERPSAGRLLIAGRDTGQLDEAALTALRGRSIGFVFQ
                     FHHLLPAFTAHENVMMPALIAHGVADPQIEADALALLDAVGLKAHAHKKPAQLSGGQQ
                     QRVAIARALALRPRLILADEPTGNLDTRTADDIFALLREFNHQTRCACLIVTHDPRLA
                     ARCDRTVTLVDGRLVI"
     gene            complement(19717..20313)
                     /locus_tag="PKX01_06915"
     CDS             complement(19717..20313)
                     /locus_tag="PKX01_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75893.1"
                     /translation="MRRPFVFILATLCSAPLAVLPAAAHARERLVHPNPAGGTTARHV
                     DAGIGPHGTTFAHGRRSVSDGTGQISSTRAGVFRGAEGASAAHASRLTREADGALTRD
                     SRFAARSARGSLDSQGSVSRAADGTLYGDRSTTAQGSAGGSYAGSTHYDQDSVSHSSA
                     ATRDDGTRYTGTTAYSRGEGVSHSGGCSDPSGKPVACR"
     gene            20521..21894
                     /locus_tag="PKX01_06920"
     CDS             20521..21894
                     /locus_tag="PKX01_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HMZ75894.1"
                     /translation="MSDTRELAKEALGTFESAIMGIAGTAPAFSVAVTTAAIVASVGV
                     LSVGSILYCGLIMFGIMLAFVHLSKISAHAGAAYAWVGEVFGPVWGFFTGWGVLVAST
                     FFMVSATIPAATSTLVILMPDKVEDTLWVTSTAALWLTAVTAVVTKGIKHASYAQVIL
                     TVIETVVVLALIVAAFLEYGSRPAHPPSMIWFSPFAFTPQIFATGALTAIFFYWGWDV
                     TMNLSEESKQAAGGHTAAGQGAFWAMVNLILFFVIMMIVVLIVLNDAEIAEANTNVLY
                     AIANKLFAPPWNYLAVLSTILSTVGTIETQILQFSRSMFAMARDEMLHPRYARIHPEW
                     QTPWVATVVIWGLGVVLLFASSALPSVKVILQSSILAIGFQICFYMSLAGFACAWHYR
                     AKLGEGLKAATSYVLWPLGGALFMVFIAVYSVPTFDLITNVSGIGGLLIGAIPLILNR
                     KRVMAGA"
     gene            complement(21964..22293)
                     /locus_tag="PKX01_06925"
     CDS             complement(21964..22293)
                     /locus_tag="PKX01_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMZ75895.1"
                     /translation="METLDPLLHQPLRTQLAAFLAGAGEASFSELKRGLQVSDGNLES
                     HLKKLVAAGYVSVARDTGPGRPQTRYALTAEGSEALRTYVQALQRLLPLAEPNLPDTA
                     GGTLADA"
     gene            complement(22305..22703)
                     /locus_tag="PKX01_06930"
     CDS             complement(22305..22703)
                     /locus_tag="PKX01_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75896.1"
                     /translation="MHRLAALALVSLVLPGCASLIRPSDAELAQLPVVRFGDAAPAGQ
                     PYILHYPAGAPLPVEARIHGSLMEQDTRSTLQVSIRKDIYTYKQWASLDGKTWCRATD
                     LIGGHFQFVVPGEGDSRAPGVLRAEFNLKP"
     gene            complement(22729..23778)
                     /locus_tag="PKX01_06935"
     CDS             complement(22729..23778)
                     /locus_tag="PKX01_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75897.1"
                     /translation="MADPIARQLDAIQSMLLRGQRNLRMERHSLILWGLAGALLFACS
                     DTVLTAEQIPDASRRATAWLLFISTVLGGVGVADWHLTRRVKQARDETWSFIHRQVVK
                     LLWLLMGVGTLFTFATFFYGGAYMLCTVWLVLIGLALYVHGLFSEEVLEWAGGTIILI
                     GVAGLVARLPFETMKWIAIAVFGLGLPLLSALLDHGRTRSFGVRLAQSAGWTVLVLAA
                     PLAAHRYASTSLPPELPVMSLAEFMAGGTMTGERIVQLPAGTRVPVRVQVSGDLFRPD
                     PDAVLPLVLDRPVELLLRDGALTGDVRAPSGPWQLARETRWISIPWIRAGLEAGRGPS
                     VDTALVVDFHGHPFH"
     gene            24158..24838
                     /gene="bluB"
                     /locus_tag="PKX01_06940"
     CDS             24158..24838
                     /gene="bluB"
                     /locus_tag="PKX01_06940"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HMZ75898.1"
                     /translation="MSDTPPQRFSDADIAAVYRAIAERRDMRHFRPDPVAPALLERLL
                     WAAHHAPSVGYMQPWRFIRITDRALRERLHALVEVERCATARALGEREDEFMRLKVEG
                     VREAGEVIVTALADGRERHIFGRRTLPEMDLASVACAIQNMWLAARAEGLGLGWVSIF
                     DPAEVATLLGLPEGARAVAILCIGHVEAFYPRPMLEMEQWAERAPLATVVYENAWGRT
                     GTGPADPA"
     gene            complement(24884..25867)
                     /locus_tag="PKX01_06945"
     CDS             complement(24884..25867)
                     /locus_tag="PKX01_06945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HMZ75899.1"
                     /translation="MFKAILIEKDEAGYRAGVRELDDSALPEGDVTVRVAYSCLNYKD
                     GLAITGKGPVVRKFPLVAGIDLAGTVEASSHPGIAVGDAVVLNGWGVGEGHWGGLAQR
                     ARLRGDWLIPLPGAFTPRQAMAIGTAGYTAMLCVMALERHGVTPAQGEVLVTGANGGV
                     GSVAIALLAKLGYTVVASTGRLDEAGFLKSLGAAEVIDRAPFSAPGRPLAKERWAGAV
                     DTVGSHTLANVCASMRYRGTVAACGLAQGMDLPATVAPFILRGVTLAGVDSVMCPRAE
                     RIEAWNRLARDLDMSKLDLITHEIGLAEAIPTAAALLAGQVRGRVVVDVNR"
     gene            complement(25969..26487)
                     /locus_tag="PKX01_06950"
     CDS             complement(25969..26487)
                     /locus_tag="PKX01_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ75900.1"
                     /translation="MSSPPLPYLLGYPEAVRTQAAQALATGRLGPWLQQRYPDTHGLR
                     SDTALYDYVAALRNRFLRTAPPLAKVRYDGTLQTVHQALGTHTRVTRVHGGRLRTKRE
                     IRIATLFRVAPEPFLHMIAAHELAHLREPEHDKAFYQLCCHMAPDYAQLELDVRLYLT
                     HLDAAGAPLWTP"
     gene            complement(26557..27291)
                     /locus_tag="PKX01_06955"
     CDS             complement(26557..27291)
                     /locus_tag="PKX01_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HMZ75901.1"
                     /translation="MASEFQLSVCPHDTAKNLLGWFTLNTYLQRKLGIGIHFEPQDNF
                     LVERQRVLDARFHVVYANPYSALCFARDKGFVPVARPAGVVDEAVIVARTEGAVPDAP
                     TVASATDKLIIHDLGLQVLRTLGIDPAAARFEFAGNHLNAAKAVIDGRADLGIVFNET
                     WAGLSPATRASLRVLGESTDGVAFHCFMVSPEWADKRADIQAILCGMHEDPAGRKVLD
                     DLRFSAFAPVGEECLPPLQALLAAKG"
     gene            27455..27871
                     /locus_tag="PKX01_06960"
     CDS             27455..27871
                     /locus_tag="PKX01_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75902.1"
                     /translation="MSGRGAGGRAPFAYLTRLPPGKAVLWCYLIWYLSTVAHHFDPSP
                     KLWLNALGISGVIGIALMLSVAGTTRPDRWQTARLFMMPFGVSSFSALIKGQGFVLIF
                     PPTWAECAVSAGACALFLAVVAAAKALARQPGTTPQ"
     gene            complement(27865..28143)
                     /locus_tag="PKX01_06965"
     CDS             complement(27865..28143)
                     /locus_tag="PKX01_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75903.1"
                     /translation="MLKTVRQFVFARAWLTFVVLALSFVAFGIASLNLVTLLKANLAL
                     FLEHGSMAAMDGALGQLLDLLLSTVLSMVFYLVFKSCEYALVRRIGGY"
     gene            complement(28201..>29102)
                     /locus_tag="PKX01_06970"
     CDS             complement(28201..>29102)
                     /locus_tag="PKX01_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HMZ75904.1"
                     /translation="PPVPAAAVSAPLADADQPPPQDAFGLPRREWGDDPLSSRWELEA
                     DDKHGTFLFRPYKPSYILPVRWSDARNPTPTSPRLGTAATSGTLDSVEAKYQLSFKTK
                     LWEGLFGRHGDLWLGYTQQSSWQVYNKGNSSPFRETDYEPELMAVFRTNRDLGAGFRL
                     RLLSIGLNHQSNGRAGDLSRSWNRVMAQAGVERGDFSLIVRPWVRLHENAGNDDNADI
                     THYLGHGDVQAVWRFSQQTLGLNLRGNPTAGRGSAGVDWSFPIHRNLKGYLQVFSGYG
                     ETLIDYNHRQNVIGFGVSLVDWM"
BASE COUNT         4136 a         9759 c        10717 g         4390 t
ORIGIN      
        1 acggcggcct gggcgcagtc ggcgcccttg acctgcatgc gggcgagggc ctggtcgtcg
       61 tcctcggtgg tgaggatgcc gttggcgatg ggcacgccgg tgtccagggt cacgcgggtg
      121 atgccggcgc cctgctcgtt ggagaccagc tcgaagtggt aggtctcgcc gcggatcacc
      181 gcgccgaggg caaccagcgc gtcgtagcga ccactcttgg ccatggtctg cagcgtgagg
      241 ggcgcttcga gggcgcccgg cacggtggcg atgaggatgg cgtccggcgc cacgcccagg
      301 cgcagcagtt cggcggtgca ggccgacagc agaccttcgc acacgtccag gttgaagcgg
      361 cacatgacga tgccgatgcg caggccctgg ccgtcgaggc tgggggcgat ttcggggatg
      421 ttgtcgtaac ggggcatggg atgtcctgcg gaaaagagcg agggcggccg cgctcaggcg
      481 tcgggcgtga cgtggccgct gagggtgagg ccgaagccgg ccatgctggg gatcttgcgt
      541 gggctggcca tcagcttcat cttgccgacg tgcagctcac gcaggatctg ggcgccgatg
      601 ccgaacaggc gcgggtccca cttctgggcc ggccgcggca ccgtggtgcc gagctggtcg
      661 agcagatcct ggccgctctg ggggcggtag aggagcacca gcacgccgtt gccgtgagcg
      721 gcgatgcggc gcagggcgtc gtccaccgtg aagctgtggc ggccggcggc ggggtcgagg
      781 aagtccagca ccgagacggg ttcatggaca cgggcgaggg tctccacgtc cgggcggatc
      841 tcgccatgga ccatggccag gtgcacctcg ccgctggtgg tgtcctcgaa ggcacacagg
      901 cggaaggcgc cgtggggcgt gtccacggtc ttctcgctga cccgcgtgac gagcttttcg
      961 gtgcgcgcgc ggtattcgat caggtcgcgg atggcgccga tcctgagccc gtgggtctgg
     1021 gcgaagtcga tgagctgggg caggcgggcc atggtgccgt cgtcgttcat gatctcgcag
     1081 atcacggacg ccggctccag cccggcgata gccgccaggt cgcagcctgc ctcggtgtgg
     1141 ccggcgcgga tcagcacgcc gccagggcgc gccatgatcg ggaagatgtg gcccggctgg
     1201 acgatgtcgc acggcttggc gtggcgcgcc acggcggcct gcacggtgcg cgcccggtcg
     1261 gccgccgaga tgccggtggt gacgccctcg gcggcttcga tggagaccgt gaaggcggtg
     1321 gagaactggc tgcggttgtc ccgcgccatc tggttgatgc ccagctggcg gcagcgcgcc
     1381 tcggtgaggg tcaggcagat caggccgcgg gcgtgcttgg ccatgaagtt gatggcgtcc
     1441 ggcgtgacgt gctcggcggc gaggacgatg tcgccctcgt tttcccggtc ttcctcgtcc
     1501 acgaggacga ccatgcggcc ggccttgatg tcggcgatga tgtcctggat cggcgacagg
     1561 gcgctcacga tgtcgcctcc tcgccggatt cgcgccaggc ctgcatgcgt tccacgtagc
     1621 gggcgatcag gtcgatctcc agattgaccc gcgcgccggg cgcaaggcgc ttgagggtgg
     1681 tgacctggac cgtgtggggg atcaggttga tggagaagtc gcggcccttg acccaattga
     1741 cggtgaggct gacgccatcc acggtgatcg agcccttgcg cgcgatgtag ccggccagtg
     1801 ccgcaggggc gcggatcacc agttcgtggc tttcgccgat gggcgcgaac ttcaccacct
     1861 cgccgatgcc gtccacgtgg ccggtgacca ggtggccgcc caggcggtcg gccaggcgca
     1921 gggccttttc gaggttcacc tcaccggggg cgtcgaggcc cacggtgcag ttgagggttt
     1981 cgcgggagac atcgaactgg acgcggttgc cctcgcgggc gatgacggtg aggcacacgc
     2041 cgttgtgggc gatgctgtca ccgagctgga cgtcgtccag cccgagggcg ccgacgtcga
     2101 cagtgaggcg gacgccgtct tcaaggggcg cgacgtccag gatgcggccg gtggcggcaa
     2161 cgattccgga gaacatggct tggggggagg tgatggggga aagtcgcgat tttaggcgat
     2221 ttccgtccac ggcaggcagt ttgtgcggcg cgggtcggtc catcgccgcc gcggctcata
     2281 aacgggaaaa gccccggacg gggcttttcc ggtggctggg cggaaggctc agaggatctc
     2341 ggtaatgtga tcccggtcct ggagttcctt ctcgaattcg accatttcgg gacccagggc
     2401 gttgcgcgcc gagaccatcc cgatgggcag cccgccttcg acgaccggca catggcggaa
     2461 gccaccctcg aacatcatgt gcagggcgtg ggccagaggc tggtccgggg aggcggtctg
     2521 cagcttggcg gtcatcaccg cggagagcgg ggtggtgtcc ggatccagac tacgggcgac
     2581 gacgcgattg agcacgtccc gctcggtgaa caggccttca agcttgccgg ccgcgtccac
     2641 gacgacgatc gcgccgatgt tcttctgggc catcgtcacc gcggcagcgc ggacggtggt
     2701 ggatgcgctg gcggatacga cggtctgacc cgctacgact tcgcgcagtt ttcgatgcat
     2761 ggttgtctcc tgatgggggt ttgatgggga aaacggtgcg ggggaacgac gcacaaaagg
     2821 ggcgctcctg gcgccccgtg cttgcgacgt tcaggtccct gtcttgggac cggccggctt
     2881 gctcatgtct ggcttggaat tcttggtggc gtggtcgccg caggcctggg cggtcgtggc
     2941 gaaacccagc gcgagcgcca gcagcagtgc gatcgtacga gtcatgacgg tgtctcctcc
     3001 gtgatgcaac gatgcttcat catggaacgg atcgcaggga tgcgcaagag gggggcgcgc
     3061 gcggccgcgc cgtctcccag tcgcgatgcc ggtcagttcc ggaagggctg tcacggggac
     3121 cggtcgtcgt gctcaggcaa cccgtcggag gggtgccgga cccgcgccgt ccgtacggtg
     3181 ggcgccagac ggaagtctgc cgcgccggtt ccgtcaaccg ccgtcggcgg cgagatgcca
     3241 cacctcgccg atgggccggg cggcgagatg cccgtggcgc agccactgca acgcgatggg
     3301 ccacaggctg tgttcgaagc ggctgtgaaa gaacgcgaag tggccgacgg tccgctgtcc
     3361 gaccgcctcc ggggtgaggt gcagatgcgt gcggtcgctg cgccggtagt agccgagcag
     3421 ccggcggatg gcctcgacgg tcccgaattc gtcatcgctg aggctcacgg cgagggtcgc
     3481 ggcgcgtacc gcggcaaagc gttcgacgag ggcgcggcgt tcggcttccg gcaggacccg
     3541 cgcgccgctg cgccagctgt cttcaaagcg gggctggcgg gcggtccagt cgcgcaccac
     3601 gccgcgcggc gtgtcttcca gccaaccgag gcgccgaccc gggaaatagc cgaagacggc
     3661 ggtgagcacg ggcattgcca cgtgccactt gaggagcaga ccggcacgcc gcggtcgggc
     3721 gtagtcgcgc caataggcat attgggcacc cattgtgaag atccgcgcca cgcacgggct
     3781 ggacggtgcc agtccgatga ggaagccgcc gacgctgtgg gccgccacgt gcaccggctg
     3841 gccgggaaaa cgggccgccg cgaagcgcag ggcgccctcg aaatcgagcc ggccccagtc
     3901 gatccagccg gcctcgaagc cgcgcaattg cggcgggcgc gattcgccaa tgccgcggta
     3961 atcgtaggtg atgacgtcaa agccgtggcc gtgcaaccag gtggcgaagc gagcgtagta
     4021 gcggcagcgc acggaggtgg cgggattgat gatcaccacc gggcgggtcg ctgcggcttg
     4081 ggaatggcat ccggcaaaac cgcgcaggat gtagccatcc gcggcgggga aggcgatggg
     4141 ctgcagtggg gcgttgccgg tggacatggg cggatgcggc gaggctggga cgggggaatg
     4201 ctaggtcggc cgatccggtc gcgcaaacgg aactccggca tgggcgtcat gcggtctgcg
     4261 catgggcgct ccgcgcgcgg gtggcgtcag tcgtgcaggg cgtgggccac ttcggcgagc
     4321 gccgcgtcaa gggcgtccat cagctggctg cagcggttgg cctcgggatc cggcccccgc
     4381 agcacctcgt ccagttcgat tgcgaccgcc gccacggtgg tgagccccac gttgccggcg
     4441 gtgcccttga ctgtatgggc aatgggcttg gcgcccgaga agtcgcccgc agcaaggcgt
     4501 tcgcgcaggg tcagcaggtg ctgctgcaga ccgtccaggg cgccctcgag cagatggagg
     4561 tagcgctcgc ggttgttacg ggtgatgcgc aggccgcggg ccaggtccac gccctgtgcg
     4621 gtggcgaggc ggtcctcggg ggctgctgcg ggcgtggggg ccggctccac ggatggcggt
     4681 gtcgcggtgg aagcgtcgat cggacctgcc gccgctggca gccagcgcag cagcgtggcg
     4741 taaagggcgg ccggatccac cggtttggcg atgaagtcgt tcatgcccgc cgcctgacag
     4801 gccgcccggt cgtcatcgaa ggcgttggcg gtcatggcca ggatcggccg cgcagcccag
     4861 ccgggcaatg cacggatctg ccggcaggct tcgaggccgt ccatctcggg catctgcatg
     4921 tccatcagga tgaggtcgta cgtgttgccg tgggccatct ccacggcgac gcggccgttt
     4981 tcggccacgt ccgcgtggag gctgacgcca tggagcagct ccagcgcgac ttcgacgttg
     5041 atccggttgt cctccgccag gagcaggcgg gcgccagcat ggcgggcgcg cagcaggtgc
     5101 tcggccgagg cagcggtgtc ccgagccggg gcctcgggcg gcggcatgac gccccggccg
     5161 cggcgcagcc aggcggtgaa ccagaagcgg ctgccctgcc cgggtgtgct ttcgaagccc
     5221 gcttcgccgc ccatcaggcc ggccaggcgt cgcgtgatag ccagcccgag gccggtgccg
     5281 ccatgctcgc gggtggtgct ggtgtcggcc tgctcgaatt cgtggaacag gttggcctgc
     5341 ttgtcgaggg ggatgccgtc gccctgatcc tccacggcga agcgtacgtg catggtgtct
     5401 ccggcgtcct gcagcagctc ggcgcgcagg acgatggtgc cgtgacggga gaacttgacc
     5461 gcattgctgg cgtagttgag gagggcctga cggacgcggg tgacatcgcc ccgcagccag
     5521 cgcggcacgt ggtcttcgtc gatgacgatg cgcaggcctt tggacgctgc ggattcgccg
     5581 acgagggagg caacgtggtc gaggatctgg ccgagggcga agtcctgatc gtcgagggtg
     5641 agcttgccgg cctcgatctt ggagaggtcg aggatgtcgt tgatgatggc gagcaggtgg
     5701 cggcccgagg cgtcgatctt gtcgagccgc tcggcctggc ggggggcggg ctggtcgcgg
     5761 cgcagcaggt gggtgagacc gatgatggcg ttgaggggcg tgcggatctc gtggctcatg
     5821 ttggccagga aggtgctctt ggcccgattg gcggcttcgg cggcatcctt ggcatgggcg
     5881 aggtcgcggg tacgctcgtc caccttggcc atcagggtgc gctggttgtc gagcagggcg
     5941 ccttcggcct gtttgcgggc ggtgatgtcg aagccgatcc cgagcacgcc gaggaggctg
     6001 ccgtcgggac cccgcagggg cgtcttggtg gtctccagca acacgcgccg gccgtcgctg
     6061 gcgtaggtga cccattcttc attgacggac ggaccgcccc gctccgtggc gcggcggtcg
     6121 tgctcgcgga agaagtcggc cagttcgcgg ggtacgaagt cgtagtcggt gcggcccagg
     6181 atgtcgcctt cggtcgcacc gaaaaagcgc tcgaattcgg cgttgcaggc cagatagcgg
     6241 ccgtcggggg ccttcagcca gatcagtgca gggacggtct tcagcacggt ctcgaggaag
     6301 ctgcgctccc gcgccagctc ggccgtgcgt tcggcgacgc ggttttccag gtcgaggcgg
     6361 gtctccgcga cccgttcggc catctcgttg aaggccgtgc ccagatgggc cagttcgcgg
     6421 cagtccgccc gatcgggctc gaacggaggc gccggttggc cggcgccgac cgcgtccacc
     6481 acccgcagca gctcgccaag cggtcgcgtc aggcggcggg cggcgtccca caccaggatc
     6541 agccccagca cgaggaaggc gccgcccagg gcgacgagcg tgtcggtgac ctccttgacg
     6601 cctccgagca tgtcttcctg acgctggaag tggacgaggc gcaggccctg ggtgccgttg
     6661 atcggaacgt ggcgatagct ggcgaccacc tcgtcgagcc ccgcaacggt cagccgcagg
     6721 gtgccttcga atccggtgct gacgccgagc aggggccgga tcgtggtgtc ggtgccgggg
     6781 cccagggtgt tgacgaggac tgcgccgccg gtgtcggcga gcaaggtctg gcgacgcgcc
     6841 tcgtccggcg cggtgtcgtc tccgctgtcc tcgaagagga gcagatcctg gttgaggaag
     6901 gccaccagat agcccaccag ctgcgtgccg ggcgtgccgt ccggtccggg agcgagcatc
     6961 tggcgggcga tgaccagctc gtcgcggccg ccgccgcgga ccggcagcag gcggatcagt
     7021 tcaaggatgc cgggctcctg cagcgtgtcg aggagctctg gatgggcgaa tgcctgtccg
     7081 gtctgtccct tcccgaccgc accgagcagc gtgccggatt ccgccgagat cacctggacc
     7141 gcccggtagt cgtccgggta ggcgcgcatc aggcgttcga gtacccgctc gatgtcgcgg
     7201 gccgcgtcgt cgtcgcggcg ggccatcagg tggccggtga tgacgcggtt ttccgcgagg
     7261 gtgagcaggt tgccgcggtg ggcccgcagg cgcgacacca gacgcgcgcg ctggaagtcg
     7321 gccccctgct cgagcagttg gatcgaggtg ccgatgtagc gctgccgttc cccctgcagg
     7381 ccaaggcccg gcacgccgaa gtaccagccg gccagaatgg cgcagatcgc cagcgtgaac
     7441 agcagcgcaa agctgcggct gatctgtgcc gaaaagctgg gcaggcggga cggcgggcgc
     7501 acgatccggt cagtcggcgt agtcgaactc ggcaagcctg aaacggcgcg gctcggcaag
     7561 gacctggtag agcccgtcgt agcgcagggc gtcggcgatg gcgtcccagc gtgcttcggg
     7621 gggcagcttg ccctgacccg cgaggaagtc gaactccttg cgcaagggcg gcgacgtgct
     7681 gcggcctgga atggcgggcg ccgaggggac gtcgaggatt tcctcgtggg tgatgcgggc
     7741 gatctgacgg tcggagatgg ggggcgcctt gcccgacagc gtctgggcgt cgaggcgtgc
     7801 ccaggacacc gcccgatcca ggttcgcctg ggcgcggcgc atccactcca gcgcccgcac
     7861 aaagccggcg atgaggtgcc gggcggcggc aggctgctgc tcggcgaagc gccggtccag
     7921 caccaggaaa tcgctggtca ggccgcgaaa gacgatccgg ttggcgcggt tggccgcgag
     7981 cgctgcgctc ggtgccggct cccaggcggc aaaggcatcg atctcgccgc gctccagggc
     8041 ctcgggcatc tcgctcaccg ccagcggtac gaggcgcacc tgacgctcgg acaggccggc
     8101 cgagcgcagg ccctgcagca gggtgtgatg ggcgctggag ccttccacgt agccgatgcg
     8161 tcggcccgcc agatcgctga cctgcgccac ccggcgaccg acgatggcgg tggacgtctg
     8221 cttcaccagc ccgacgatga ccgcctggcc tcgcgcgcac gccatcagag tcggcatgtc
     8281 ccccagcagg ccggcctcca ggcgccggtc ggcgagcagg ggcacgatat cggcgccacg
     8341 ccagaaggcg tggaagcggg gaggcgtgcc gagggattcg agggcctgcc gcaggatacg
     8401 atcgcggctc atcacggcgc tgatgacgcc ggagggcacg ccgaggggct ggatgcccac
     8461 gtccacctcc ggcgatgcgg gggacaagcc gacgggcggc gtgtcccgct cggccagggc
     8521 aggcatcgcg atcgacagga ccagcaggcc caggagcagt ctcggtcgca cgtcggagtc
     8581 tccgatccgc tgtgaggatt gacgagcttg tcagttcaga ccgttttaac ggccgagcgc
     8641 gacgataacg ttaattattc cgaaaaacca gtggcattct tgtggcctcg gggcgcgtct
     8701 tccgtgcgga gggcgttggc ctggagtaca gtcgccggcg ctggcggaag cgccctgccg
     8761 cgccgggccg tcggctgtgt gtgtgggacg gcggccgcgc gcctgcgccc ccgcgtccgt
     8821 gaatgcccgt caaggagacc cgaatggaca gtctgagcgc agccctgcaa cgcctcttcg
     8881 gccggcccgg ccagcctctg cccgatccgt ggccggagtc cggcgcgctg ctcgaaccgg
     8941 tggacccgga cgggcgcgcg tgggccctcg tgatcggtct gccccgcccg gccggcgggg
     9001 cgatggtgcg ggccctgtgc gagggggcga cggctctcga tctgcccgtg ccggcagttt
     9061 ccctggcagg ggacgcaggc tttcacgtgt ggtttgccct gaccgacccg gtgccggcgg
     9121 agcgcggccg ggtcttcctg gaggccttgt gccggcgctg cctgcccgag ggcgcgtccc
     9181 gagcgctgcg gctgcccggc accgtgggcc tgacgggcct gtccctgccg cccgcccgcg
     9241 acggcggacg ctggacggcc ttcatcgacc cgtccctggc cggcatgttc gatggtgaaa
     9301 cctggctgga tatggcgcca tctccagagc ggcaggcgga gctgctcgcg ggtttccgcg
     9361 ccatccgccc ggcccagttc gaccgtgcgc tggcagaact cgatccaccc ggcgccgtgg
     9421 tgccttcgcc gcggggcgcc ctggccgggc aggtggtgga gccgcctgcg ctgagcggga
     9481 cggtgccgcc cctcgggctg ggtgtgggga gcggcttcgt ggacccgcgc accttcctgc
     9541 tcgccgtgat gaacgatccg ggcgccagtg ccgatcaccg catcgacgcg gccaaggccc
     9601 tgctgcctta tttcgaacgc tgatggcggt ccgtcaccag taccgcggcc agtccgcgtc
     9661 ccgcgccaga ttgttataga ccagcgccac gcccaccagc agcagggcgc cggccagggt
     9721 cggcagcagc aggaaggacc agcccggctg gagcagaaag acgatcaccg gattcgagcc
     9781 cgcaggcgga tggacgcagc gcagcgccat catgcccatg atggccagcg ccaccgccag
     9841 cgccgcggcc caccagtgcg gcccggccag actcaggcag gccagaccga cggccgagct
     9901 caggacgtgg cccagcacca cgttgcgtgg ctgggagaat ggcacctccg gaaagccgaa
     9961 gaccagcacg cagctggccc cgaacgagcc cagtacgagg gcggcggcag tgcgttccgc
    10021 caggcccgcc accacggcga tggcgaggaa gccgccgaga ccggcgagca ggatggcgcg
    10081 cagcgggggc gccggcggca gcggcgcggc gtggccgcgg agacggtgca ggaaagcagg
    10141 catggcggag ctccttgaga gggtcaggtc aggcgcggcg cgggggcggc cggtccgctc
    10201 gccggtcggg cgatgctacg ggtgggccat gtccctgatc tgccggacct gaaggagaca
    10261 gatctgtcta catcatccca tggcgcatcc gccggtgcaa gccggacgaa tgaccggcgc
    10321 ccgcaggtcg ggctttgcaa gcccgggtcg gcgcggtaag gtctgagcat gaccgctgcc
    10381 cccccctccc ctgccgaatc cgctgccccg atgcattccc gctccgagcg ccgggcccat
    10441 ctcgtggaca cggccctgcg cctgttcgcc cgcggcgggt accacgccac cggcatcgat
    10501 gcggtgctgg ccgcgtccgg cgtggccaag aagaccctgt accaccattt tccgtccaag
    10561 gacgatctga tcgtcgaggt gctgcgcgaa cgggaccggc gcttccgggc cagcctcgcc
    10621 gccttcgttg cggcccgcgc cacgccccgc gcgcgcctgg ccgcggtgtt cgaatggcac
    10681 gccctgtggt ttgccgagcc ggatttcgcc ggctgcatgt tcctcaatgc cgcggcggag
    10741 ttccatgacg ggtcggccgg gatcctcgcc gtcgcccgcg aacacaagca ggcgctggaa
    10801 gcctggctgg ccgcgctgct caccgatctg gtggaagcgg agcgcgccgc ccccctggcg
    10861 gccgacctca acctcttgct ggacggcgcc atcgggaccg ccctggtgag ccgcgacccg
    10921 gcggccgcag cccgcgcctg ggcgctggcc gagcgcctgc tgcccgcggc gtgaggtgac
    10981 tgccatgcgt cacgccgtgt tcgttctcct cctccttgcc agcgcgtggc cggcccgcgc
    11041 ctccgatgaa gcccgtctgc ccggcgacgc cgccgccgtg cgtgccgccg atctggaaaa
    11101 gcccttcaaa ctgagcctcg gcgagtaccg ctacggcggc aacttcagcg gccaggacgt
    11161 gaacctgcgc tatcgccgcg gcgacagctc ggcttggctg ggcagctacc gtgaccggga
    11221 cttcggcacc cagacccgcg ccggcgcgga caccagctgg cagcccttcg ccggttccac
    11281 gctggccctg cagccctccg tgcagctggc ctcgggcggt ttcgtcggcg gcagcttcag
    11341 cgtggaactg ggcgaaccct ggttcgtgca ggcgggtatc gggcgcacca acacgcggcc
    11401 gtacttcaac ctgaacttcg accccaacga cgcccagtcc tacgccgtcg gctatcggga
    11461 cgacagcggc cagacttact acctgatgag cgtccgcgac gaccggctcg gcacccgcca
    11521 gcgtcacacc cacgccgttg cgcgctggcc ggcggggccg acgcggctca cggtggacgt
    11581 cctgcacaag actggcgacg gcgacaacgg ccacgtggac gcctggggcg ccaccctggg
    11641 cgtcgattac ctggactggt tcgtgcgcct ggtgcgggat ccgcaccaga acttcggcga
    11701 cgccaacgcg acgcgcctga ccgtggggcg acgtttctga gacccgccgc gctgaccagg
    11761 tcacgcagat gaagcctggg gcgctgccta cagcgcgtcg agccccacca gcgcccagcc
    11821 gtcccgcgtc agcgcgtccc gcaggaagcg ggcggtggcg accgcgtccg gaccgtcgcc
    11881 gtggacgcac acgctgccga tgggcgtcgg ccggcgcacg ccgttcaccg agatcagggc
    11941 ctgctcggcg agcatcgcgc tgacgtgggc gagggacgcc ccggcgccgt ggatcatcgc
    12001 gtccgggcgg ctgcgcggca ccaggctgcc gtcgtcctgg taggcccggt cggcgaagat
    12061 ctccgccacc acccgcagcc cggcctcggc gccagccgtg agcagcgccg aacaggccgg
    12121 ggcgaggagg atcaggtccg cgtcgaaggc gcggatcgcc gcgaccaccg tgcgcgccag
    12181 cgccgcatcc gtgcaggtca tgttggacag ggcgccgtgg ggtttcacgt ggcccatctt
    12241 cacgccttcg gcctgcgcca tgccggccag cgccgccatc tggtagatca gcaccgcctc
    12301 cagttcggcc ggcggcagct gcatggggcg gcggccgaag ccctgcagat ccggaaagga
    12361 cggatgggcg ccaacgccca ccccggcggc ccgggcgtgg cgcagggtcg tgcgcatcac
    12421 cagcggatcg ccggcgtgaa agccgcaggc gatgttggcg ctgttcaccg tggcgagcag
    12481 ctcggcatcg ctgcccatcg tccaggcgcc gaagctttcg cccagatcgg cgttgagatt
    12541 gatgcggcgg ttcatggtcg gtgtctttcc tccgggttcg tcttcaccgc gcgtgcggtg
    12601 gcgaggggcg atgcgcttcc gggccgcaac ggcccgtgtg tcagtcctcg tcgccgcgca
    12661 gcacaccgcc gaccaggttg gcggcgtaga gcgccgttgc atcgaagccg ccggcgggtg
    12721 gtagcgcgga gagggcggct cgccaggcgg cgaaaagccg ggcctgttcc tgccgggcgc
    12781 gggcggctgc ggcggcgtcc acggcgctga agctgagcgt gtcgccgggc cgcaggtgac
    12841 ccaggcgggg caggtcggcg gcgatgacgg tggcaatctt ggggtatccg ccggtggtct
    12901 ggcaatcggc gcgcagcagg atgggctggc cgtccgccgg cacctggatc gcgccgggcg
    12961 tcacgccgtc ggtgacgatc tcggcgcccc gcgcgctgtg ggtcaggcgc gggccggcca
    13021 ggcgcgcgcc catgcggtcg gcggcgggcg tgacggtcca cggcccggcc aggaaggcag
    13081 cgaagcaggc ctcgtcggca tgcgcgcgct ggggcccggc gatcacccgc agcggaccgg
    13141 cagggaggcg ccacggcgga ccgacccgtt ccggcccgtc cggggccgca ccgcaaggca
    13201 ggcggtcgcc agcctggatc gggcgtccgg caatgccccc cagcccggcg cgggcgtagg
    13261 tggcgcggct gcccagcacg accggcaccg tcatgccgcc ggacaccgcc agcacggcga
    13321 cgccggcttc cacgcgcccc accgcgagga tgtcgccggg ggtgagggtg gcgctgcgcc
    13381 agggcggcag gggctcggcg ctgccgtcgc cgcgcagcac gcggctcgcc acggcgccgg
    13441 cgagggcgac ccgtaccgga ccgtccgcgg cctgcaggcg cggtccgtgg agcaggatct
    13501 ccagcgctgc ggcgtcagcc ggcgcgccgg ccagggcgtt ggcggcggcg agcagcagtg
    13561 ggtccagggc gccggagacg ggcacgccca gggcgcggta gccgacgcgg ccgcgatcct
    13621 gcacgctgac gccgaggccg ccgtcgagga cgtggaggtg ggtcatgggg cggcccgcag
    13681 gcgatccggg tcagcgcgtc cggcggtgaa gtcggcggcc agcgtgtcga agtcggagcg
    13741 cgtcacggcc tgccagcgca cccggtcacc cggcgccagc aaggccggtc gcgcgctgcg
    13801 gcggacgtcg aagcagggta ccgggcagcg tcccagcagg cgccagccgc cggggctggt
    13861 ccatggatag acggcgcaca gggcgtccgc cacggccagg gagccggctg ggacggcggt
    13921 acgcggtgtg gccaggcggg gctgtgccag ctccgccggc aggccgccca ggtaggggaa
    13981 accgggcagg aagccgagca tccacacgct gaattccgcg ccggtgaggc gggcgatcac
    14041 gccggcgcgg tccagcccgc agcgtgcggc gaggtcgtcc agatcggggc cgaaatcctc
    14101 gtcgaagcac accggcagcg tccaggtcgc gccgttcagc ggcacggcgt gggtctggcg
    14161 ggcgaggctc agcaggtgcg cggcgagggc atctggatcg gcgttgtccg ggtcgaagaa
    14221 cactgtcacc gacgcgaagg ccggcaacca ttcgatcacg ccgggcagcg cgcccgcgtc
    14281 cgccgccgcg gccagtccgg cggcgaagcc ccgcacgcgg gcgtgcaggt ccggcgcgat
    14341 ggtcgtgccg aattccaccg tgaaggcggt gtcgccgagg ggcagcaggc ggggggcgtt
    14401 gggcgtggcg gacatgggcg cgggggctgt gcgcggcatc ttacgcgccg tctgcggggc
    14461 gttccaggcc ggtgcgacgc cctcgcgtgg atgaagatgc tggcgccgcg ggtccgggaa
    14521 gatcgcctcc gtcgggcagg acggcgcctg tcgcgaaagc ggatggacga gacccgggtt
    14581 tcgtctgtgc caatgagcac ccggcacagc gggtgtgagc ctgccggtcg cacccgccgg
    14641 gcgggttgcc gcgtaccggc gcgcggcgcg gcgcttactg cccgagggcg ccgaaggcct
    14701 tcttcgccag cccggtggcg gtgcccagcg gatccttgcg gatcgccttc tcttcctcgg
    14761 cgatcatcag gaacagcccg tccagggcct tgcgggtcac gtagttctcc accttcaggt
    14821 cgtccccctt gcccaggccg aagctgccgg ccttgcccgc cagggcgttg tactgctggg
    14881 acagggccag cttgtcggtg gtggccttga cggtgggcag gaaggactcg aagagctggg
    14941 ccgaggtctt ctccttgaag tactgggtcg ccgcgtcgtc gccaccgccg aggatcttct
    15001 tcgcgtcgtc cacgctcatc ttcttcaccg catccaccag cagcaccttg gccttgggca
    15061 cggcctgctc ggcggcgtgg ttcatggtgg ccacgaggct gtccagatcc ttgccgcggc
    15121 ccatggtgcg cagcacgcct tccaccggcg cgaggctctc cggcaggccg atcttcactt
    15181 tcgggttgtt gaggaaacct ccgtcggtgc ccagcagtcc aacggccttg gatgccccct
    15241 ggctcagggc ctctcgcagg ccgccggaag cgtccttgtc gctcaggtcc gccaggccga
    15301 aggcccacgc ctgggtgcag atgacaagca gcagcaaagc tttcagaatc ccacgcagcg
    15361 tacgcatcgc ccaatctcct gaccggtctt aaaacgaatg ttgcccaagg ctatcacgcg
    15421 cttcattgcc gccctgctgt ttcttgcact tcttgacgga tgcagcgccc ccgccgcgcc
    15481 ggacgtgagc ttcaccaccc tcgacggcca gaccacccgc ctgtcggcgc tgcgcggcaa
    15541 ggtggtgctg gtgaatttct gggccaccac ctgcaccacc tgcgtgggcg agatgcccaa
    15601 gctggtggac acctaccggc gtttcgcccc caggggcttc gagaccgtgg ccatcgccat
    15661 gagctacgac ccgcccgagt acgtgcgcaa ctacaccgcc aaggccggcc tgcctttcaa
    15721 ggtggcgctg gatgcccgcg gcgaggccgc ccgcggcttc gaggaggtgc gcctgacgcc
    15781 caccaccttc ctcctcgaca agcagggccg ggtggtccag aaatacctgg gcgagccgga
    15841 tttccccaag ctccacgccc tcctcgaaaa actcctcgcc gatccggcgt gaagcgcccc
    15901 gtcctgagtg tcgccctgct ggcggcggcc ggtctggcgg cccttgtgct gtggccccgc
    15961 ggccgcgagg tggacggcgt cgccgtgcgc cgcgcgcctc tcacccagtc cgtcgttgcc
    16021 accggacgca ttgccacccc ggcgcgcatc accgtcggtg cgccgctggc cgccaccgtg
    16081 ctggccatca gcgtgcgtga aggcgactcc gtcaaacccg gccaggtgct ggcccgcctg
    16141 caggccgacg acgccgatgc cctggtgcgt caggccgccg ccgctctggc cgaagccgaa
    16201 gcccgcctca gccagatcgc caccgtgggc gagccggtgg ccgcccaggc cctggcccag
    16261 gcggaggcca acctgcacgt ggcccggcgc gaggccgagc gctcgcgcca gctggtcgcc
    16321 cagggcttct actcccagtc ccgggtggat gacgccctgc gcatcgagac caacgcccgc
    16381 aacgccctgg acaccacccg cgcccagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnngc ccgcgtggac caggcccgcg cccagcttgc caacgcccgt gcccgcgccg
    16561 ccttgctcac cctcaccgcc ccatcggcgg gcacggtgct cagccgcaag gccgagcccg
    16621 gcgacgtgac caccgccggc aaggtgctgc tggagctcgc cgaggccggc gagacccgcg
    16681 tctatgccac cgtggacgag aagaacctgc gcctcctggc cctcggccag aaggcccgcg
    16741 gcgtcgccga cgcctttccg ggcacgccct tcgaggccga actgtactac ctcgccccgg
    16801 ccgtcgatcc ccagcgcggc accgtcgaga tccgcttccg cgtacccgcg ccgccggcct
    16861 tcctgcgccc ggacatgacc atctccgtcg agaccgtcac cggccaccgg gacgccaccc
    16921 tggtcctgcc cagcgaggcg ctgcgtgaag ccgacggtgg cgcaccctgg gtgctcgtcg
    16981 cccgcgacgg ggtggccgag cgcgtgccgg tgcagaccgg cctcaccggc atcggccagg
    17041 tcgagatcct gcacgggctg gtcgagggcg atgttgccat cctgcccgcc gctggcgccc
    17101 ttgacggcga ccgggtgcgc ctgcgcgaga tgaagaagcc ccgcaacacc ctgcaggtgc
    17161 ccggcgggat ggcgcgatga gggcggctcg gagaggggct gagcgaggcg gggccgcgcg
    17221 ggctggaacg gcgcgagctg tcggagagcc ggtttccgcg gcgacctcca cccatggaca
    17281 aagccaagcc tgccccgata cgggcgcggt tcagcgggcg ggcgtccgcg gtaccgactc
    17341 cgtccccggt cgggaaaatc cccgctgtgc atttttaaat ggcccggccg gatttcgtcg
    17401 cctctttttg aggcagataa atgcccgcag cccatttaaa aatccggtga ccggattttt
    17461 ctgcctcaaa aagaggcaag aaaatccctt tggcgcatta tttaagggtc gtcacccctt
    17521 taaaaatccg accggtacat ttttaaatgt ctccgccgga ttaaataatg cccggcgccc
    17581 atttaaaaat ggttgccggg tattttcgtc ggcagcggcg ggggcgggct ggcggtcgtc
    17641 cgccgcccgc gtccccccgc gttccgccgg gtccgccacc ccggctcccg ccggcgccgt
    17701 ccgatgaccc tccgcctccc ctttgcgccc ctcgtggccc tgcgtttcct gcgggaaggg
    17761 cgcatgcaga ccctgctgat cctcgccggc accaccggcg gcgtggcggt gatcgtcttc
    17821 ctgacccagc tcatcaccca gctccaggcc cagatcgtgg accgggtgct gggcagccag
    17881 gcccacgtgg tgatccgtcc gctggaggag gccaaccgtc gcgccttcgc gcccgacggc
    17941 atggccaccg tcgttgagcc gcgcgcccag cgcctgcgct ccatcgacca gtgggagagc
    18001 gttgcccgcc tggccgccgg gctgcccggc gtcacggccg tgtcaccggt ggccaccggc
    18061 ccggcctttg cgacgcgcgg ccaggccaac aagtccatcg ccctgatggg cgtggacgcg
    18121 gaccgctacc ggcgcatcgt gcgcatggag gaatacatga aggccggtcg ctttgcggtg
    18181 gacggcaccc gcgcggtgat cggcgccgac ctggccaccg acctgggcct ccgcctggag
    18241 gacaagctgc gggtgatgct tcccaacggt cgcgacgagc tgctccaggt gggcggcatc
    18301 ttcgacatcg gcaacaagga cctcaaccgg cgctgggtct tcgtcaccct gcccctcgcc
    18361 cagagcctgc tggatctgcc cggcggcgtc tccaacctgg acctgacggt ggccgacatc
    18421 ttcgaggccc gccccatcgc cgagcgcctg cgcggcgcca ccggcctgac cgtggacagc
    18481 tggatggaga ccaacgccca gctcctcgcc gccctgcgca accagaccgt ctccaacaac
    18541 ctgatccgca gcttcgtggt cctcatcgtt gccctgggta tcgccagcgt gctggtggtg
    18601 agcgtggtgc agaagcagaa ggagatcggc atcctgcgcg ccatgggagc gcgccgcggg
    18661 atgatcctgc aggtcttcct gatccagggc gcggtggtgg gcgccgttgg ctcggtgctc
    18721 ggcagcgccc tcgccttcac gctgctgcgg gtgttttcca gcatctaccg caacgctgac
    18781 ggctcgcccc tgttctcgcc ccagctggag ctgcgcttca tcgtcatggc ggccctggtg
    18841 gcgaccgccg tcgggctgct ctccgccctc atcccggccc gccgcgcggc gcgtatggac
    18901 ccggtgcagg cgatccgcgc atgagcgccc tgcgccccgc cgccgccggc ccggtgctgg
    18961 aactggcggg catcaccaag acctacaacc ccggcacgcc ggtggccacc gaggtgctcc
    19021 acggcatcga cctcagcctt gccgagggcg aattcacggc gctcgtcggc ccctccggct
    19081 ccggcaagag caccctcctc aacctcatcg gcctgctgga acggcccagc gccggccgcc
    19141 tcctcatcgc cgggcgtgac accggccagc tcgacgaggc cgcgctcacc gccctgcgcg
    19201 ggcgcagcat cggcttcgtg ttccagttcc accacctgct gccggccttc accgcccacg
    19261 agaacgtgat gatgccggcc ctcattgccc atggcgtcgc cgacccgcag atcgaggccg
    19321 acgccctggc cctgctggac gccgtcggcc tcaaggccca cgcccacaag aagcccgccc
    19381 agctctccgg cggccagcag cagcgcgtcg ccatcgcccg cgccctggcc ctgcgcccgc
    19441 gcctgatcct cgccgacgaa cccaccggca acctggacac gcgcaccgcc gacgacatct
    19501 tcgccctgct ccgggaattc aatcaccaga cgcgctgcgc ctgcctgatc gtgacccatg
    19561 acccacgcct cgccgcccgc tgcgaccgca cggtgacgct ggtggatggg cggctggtga
    19621 tctgagctca ttccgccggc gtgccgcctc caggtggccg ggcgaatccg cgcgggaggc
    19681 gcccgacagt gggacgcggc gagggcatcc cgtggctcag cggcacgcca ccggcttgcc
    19741 cgacgggtcg ctgcatccgc cgctgtgact gacgccttcg ccgcgggagt aggccgtcgt
    19801 gcccgtgtaa cgggtgccgt catcgcgggt ggcggcgctc gagtggctca cgctgtcctg
    19861 gtcgtagtgg gtgctgcccg cgtagcttcc gccggcgctg ccctgggcgg tggtgctgcg
    19921 gtcgccatac agcgtgccgt cggcggcacg gctgacgctg ccctggctgt cgaggctgcc
    19981 ccgcgcgctc cgggcagcga agcggctgtc acgggtgagc gcgccgtcgg cttcgcgggt
    20041 gaggcggctg gcgtgagccg cgctcgcacc ctccgcgccg cggaataccc ccgcgcgggt
    20101 ggaactgatc tggccggtgc catcgctcac ggaacgcctg ccgtgggcga acgtggtgcc
    20161 gtgagggccg atcccggcat cgacgtggcg ggcggtggtg ccgccggcgg ggttgggatg
    20221 cacgaggcgc tcccgcgcat gggcggcggc agggaggacg gcgaggggcg cggaacacag
    20281 ggtggccagg atgaagacaa acgggcggcg catggcgagg tcctttccgg tggcggtggc
    20341 ggggatgggg cgcaggctgc agtctagtcc tggacgcgcg gtgggcgtgt gtcgcgggga
    20401 cggcgaagtg tgaggggatg tacgacctcc gccggccacg ggcgtggggg cgggagcgga
    20461 acgatgggaa caaaaaccgg tatcctccgc ccatccccaa cttccccccg gagcatcatc
    20521 gtgtcggaca cccgcgagct ggccaaggaa gcacttggca ccttcgaatc ggccatcatg
    20581 ggcatcgccg gaacggcgcc ggccttcagc gtggcggtga cgacggcggc gatcgtggcc
    20641 tcggtgggcg tgctgtcggt ggggagcatc ctgtactgcg gcctgatcat gttcgggatc
    20701 atgctggctt tcgtgcatct gtccaagatc tcggcccatg ccggggcggc ctatgcgtgg
    20761 gtgggggagg tcttcgggcc ggtgtggggc ttcttcaccg gctggggggt gctggtcgcg
    20821 tcgaccttct tcatggtgtc ggcgacgatc cccgcggcca cttccacgct ggtgatcctg
    20881 atgccggaca aggtggagga caccctgtgg gtcacgtcca ccgcggccct gtggctcacg
    20941 gcagtgaccg cggtggtgac caagggcatc aagcacgcga gctacgccca ggtgatcctg
    21001 accgtcatcg agacggtggt ggtgctggcc ctgatcgtgg cggcctttct ggaatacggc
    21061 agccggccgg cccatccgcc gtcgatgatc tggttctcgc ccttcgcctt cacgccgcag
    21121 atcttcgcca ccggcgccct gacggcaatc ttcttctact ggggctggga cgtgaccatg
    21181 aacctcagcg aggagagcaa gcaggcggcc ggcgggcaca cggcggccgg gcagggggcg
    21241 ttctgggcga tggtcaacct gatcctgttc ttcgtgatca tgatgatcgt ggtgctcatc
    21301 gtgctcaacg acgcggagat cgccgaggcc aacaccaacg tgctgtacgc catcgccaac
    21361 aagctcttcg cgccgccctg gaactatctg gcggtgctgt ccaccatcct cagcacggtg
    21421 ggcaccatcg agacccagat cctccagttc agccgcagca tgtttgccat ggcccgcgac
    21481 gagatgctgc atccgcgcta cgcccgcatc cacccggagt ggcagacgcc gtgggtggcc
    21541 accgtggtga tctggggcct gggggtggtg ctgctgttcg cgtcgtcggc gctgccctcg
    21601 gtcaaggtca tcctgcagag ctcgatcctc gccatcggtt tccagatctg cttctacatg
    21661 agcctggcgg gctttgcctg cgcctggcat taccgggcca agctgggcga ggggctgaag
    21721 gcggcgacga gctatgtgct gtggcccctg ggcggggcgc tgttcatggt gttcatcgcg
    21781 gtgtacagcg tgccgacctt cgatctcatc accaacgtgt cgggcatcgg cgggttgctg
    21841 atcggggcga ttccgctgat cctcaaccgc aagcgggtga tggcgggcgc ctgagcgcgg
    21901 gcggcgctgc ggcgcggcat cgccgaaact tggggactcg cgcgaccggc gtccgggcgg
    21961 ccttcacgcg tctgccagcg taccgcccgc tgtgtcgggg aggttcggct cagccagcgg
    22021 cagcaggcgc tgcaaggcct gcacgtaggt gcgcagggcc tcgctgccct cggcggtgag
    22081 ggcgtagcgg gtctggggcc ggccggggcc ggtgtcgcgg gcgaccgaca cgtagccggc
    22141 cgccaccagc ttcttgagat gggattccag attgccgtcg gagacctgga gcccccgttt
    22201 cagttcgctg aagctggcct cgccagcgcc ggccagaaag gcggcaagct gggtgcgcag
    22261 gggctggtgc agcagggggt ccagggtttc catcgtggcg gctgtcaggg cttgaggttg
    22321 aattctgccc gcaatacgcc cggcgcgcgg ctgtcgcctt cgcccggcac gacgaactgg
    22381 aagtggccgc cgatcaggtc ggtggcgcgg caccaggtct tgccgtccag gctggcccac
    22441 tgcttgtagg tatagatgtc cttgcggatg ctcacctgca gggtgctgcg ggtgtcctgt
    22501 tccatcaggc tgccatggat gcgggcttcc accggcaggg gggcgcccgc cgggtagtgg
    22561 aggatgtagg gctggccggc aggggcggcg tcgccgaaac ggacgacggg cagctgtgcc
    22621 agttcggcat cggaggggcg gatcaggctg gcgcagccgg gcagcacgag gctgaccagg
    22681 gcgagggcgg caaggcggtg catggacgac tcctagggag ggggaaaatc agtggaaggg
    22741 gtggccgtgg aagtccacca ccagggcggt atcgacgctg ggcccgcggc cggcttcgag
    22801 gcccgcgcgg atccacggaa tggagatcca gcgggtctcc cgggcgagct gccaggggcc
    22861 gctgggggca cgcacgtcgc cagtgagggc gccgtcccgc agcagcagtt ccacggggcg
    22921 gtcgagcacc aggggcagca cggcgtcggg gtcgggacgg aacagatcgc cggacacctg
    22981 tacccgcacc ggcacgcggg tgccggccgg gagctggacg atgcgttctc cggtcatcgt
    23041 gccgcccgcc atgaactcgg ccagggacat caccggcagc tccgggggca ggctcgtcga
    23101 cgcgtagcgg tgagccgcca ggggggcggc caggacgagc accgtccagc cggcggactg
    23161 ggccaggcgc acgccgaagc tgcgggtccg cccgtggtcc agcagcgctg acaggagcgg
    23221 caggccgagc ccgaacacgg cgatggcgat ccacttcatg gtctcaaagg gcagccgcgc
    23281 caccaggccg gccacgccga tcaggatgat ggtgccaccc gcccactcca gcacttcttc
    23341 ggagaacagg ccatgcacgt acagggcgag gccgatgagg accagccaca cggtgcacag
    23401 catgtaggcg ccgccgtaga agaaggtggc gaaggtgaac agggtgccaa cgcccatcag
    23461 cagccacagc agcttgacca cctgacgatg gatgaaggac caggtctcgt cgcgggcctg
    23521 cttgacccgc cgcgtcagat gccagtccgc cactccgacg ccgcccagca cggtcgagat
    23581 gaacagcagc caggcggtgg cccgccggga cgcgtcgggg atctgctcgg cggtgaggac
    23641 ggtgtcgctg caggcgaaca gcagcgcacc ggcgaggccc cacaggatca ggctgtggcg
    23701 ctccatgcgc aggttgcgct ggccgcgcag gagcatggac tggatggcgt cgagctgacg
    23761 ggcgatgggg tcggccatgg gcgggcgtcc tattaactct gtgacgcaga gcttataact
    23821 ctgaatcgca gagtgtcaac gcgtgcccca tggaatattc gcagggcgag accgactccg
    23881 gtccggcgcc caacgcccgc ggcgccgaac cttgccgcgg ccgggcgtgg cgcggcgggg
    23941 gcgcatcgat gggccgaggc acggcaggcc tcccgcggcc ggcggcggtc gtgacgaaag
    24001 atgccccgcg caccgccggt gacaggcatc ccgtggtccg cgaatgttcg gggccgtccc
    24061 ggaatcgggg gacggcgtgg cgccatgcgg cggctgcgac cccgtccgcg gggtccgggc
    24121 ggtatcatcc gccctcccct tccgcgtgcc cgccgccatg tccgataccc cgccgcagcg
    24181 cttctccgac gccgacatcg ccgccgtcta ccgggccatc gccgagcgcc gggacatgcg
    24241 ccatttccgc ccggacccgg tggcgccggc gctgctggag cgcctgctgt gggctgccca
    24301 ccatgcgccc agcgtgggct acatgcagcc ctggcgcttc atccgcatca ccgaccgggc
    24361 cctgcgcgaa cgcctgcacg ccctggtgga ggtggagcgt tgcgccaccg cgcgggccct
    24421 gggcgagcgg gaagatgaat tcatgcgcct caaggtggaa ggcgtgcggg aggcgggcga
    24481 ggtgatcgtc acggcgctgg cggacgggcg cgagcgccac atcttcggcc gtcgcacctt
    24541 gcccgagatg gacctggcct cggtggcctg cgccatccag aacatgtggc tggcggcccg
    24601 cgccgaaggc ctgggcctgg gctgggtgtc gatcttcgat ccggccgagg tggcgaccct
    24661 gctgggtttg ccggaaggcg cccgggccgt ggccatcctg tgcatcggcc acgtggaggc
    24721 cttctacccg cggccgatgc tggagatgga gcagtgggcc gagcgtgcgc cgctggccac
    24781 cgtggtctac gaaaacgcct ggggccggac agggacgggg ccggcggacc cggcctgagg
    24841 ccgggcgcga aggcccccgc ccgcggaccg ggcgcgttcc gcgtcagcgg ttcacatcca
    24901 ccaccacccg cccgcgcacc tgtccggcga gcagcgccgc ggcggtggga atcgcttcgg
    24961 cgagtccgat ttcgtgggta atgaggtcga gcttgctcat gtccaggtcg cgggcgaggc
    25021 ggttccacgc ctcgatgcgc tcggcgcggg ggcacatcac gctgtccacg ccggccaggg
    25081 tcacgccgcg caggatgaag ggcgcgacgg tggcgggcag gtccatgccc tgggccagcc
    25141 cgcaggcggc cacggtgccc cggtagcgca tgctggcgca gacgttggcg agggtgtggc
    25201 tgccaacggt gtcgaccgcg ccggcccagc gttccttggc cagggggcgg ccgggggcgg
    25261 agaacggggc ccggtcgatg acttcggcgg cgccgaggga cttgaggaag ccggcttcgt
    25321 ccagtcggcc ggtggaggcc accacggtgt agccgagctt ggccagcagg gcgatggcca
    25381 cgctgcccac gccgccgttg gcaccggtga ccagtacttc gccctgggcg ggcgtgacgc
    25441 cgtgacgttc cagcgccatc acgcacagca tggcggtgta gccggcggtg ccgatggcca
    25501 tggcctggcg cggcgtgaag gcccccggca gcgggatcag ccagtcgccc cgcaggcgcg
    25561 cccgctgggc caggccgccc cagtggcctt cccccacgcc ccagccgttg agcaccacgg
    25621 cgtcgcccac ggcgatgccg ggatggctgc tggcctccac cgtgcctgcc aggtcgatgc
    25681 cggccaccag cggaaacttg cgcaccaccg ggcctttgcc ggtgatggcc aggccgtcct
    25741 tgtagttgag gcaggaatac gccacgcgga cggtgacgtc tccttcgggc aatgcggaat
    25801 cgtccagctc gcgcacgccc gcgcggtagc cggcttcgtc cttttcgatg aggatggcct
    25861 tgaacatgtc tgtctcccgg tgaatggtgt tgtcagggtg gcacgcgcca cgcagcaacg
    25921 atagcccgtt gcggcaggca gggccagtgg cccgggcggc gtcgcgcctc agggcgtcca
    25981 caggggcgcg ccggccgcgt ccaggtgggt gaggtagagg cgcacgtcga gctccagctg
    26041 cgcataatcc ggcgccatgt ggcagcacag ctggtagaag gccttgtcgt gttcgggttc
    26101 gcgcaggtgg gcgagctcat gcgccgcgat catgtgcagg aagggctccg gggccacgcg
    26161 gaacagggtg gcgatgcgga tctcccgttt tgtccggagc cgaccgccgt gcacgcgggt
    26221 cacgcgggtg tgggtgccga gggcctggtg gacggtctgc agcgtgccgt cgtagcgcac
    26281 cttggcgagg ggcggcgcgg tgcgcaggaa gcggttgcgg agcgcggcca catagtcgta
    26341 gagcgcggta tcgctgcgca ggccgtgggt gtccgggtag cgctgctgca gccaggggcc
    26401 gaggcggccg gtggcgaggg cctgggcggc ctgggtgcgg acggcttcgg ggtagcccag
    26461 caggtagggc aaggggggag atgacatggg gcggcagacg gcggcggggc ggctcatgat
    26521 gccgccccga ccgggtttcc gccagcccgg tggccgtcag cccttggcgg cgagcagggc
    26581 ctgaagcggc gggaggcatt cctcgccgac cggcgcgaag gcgctgaaac gcaggtcgtc
    26641 gagcaccttc ctgccggccg gatcctcgtg catgccgcac aggatggcct ggatgtcggc
    26701 tcgcttgtcg gcccattcgg gcgataccat gaaacagtgg aaggccacgc cgtcggtgct
    26761 ctcgcccagc acccgcaggc ttgcgcgggt cgccgggctc aggccggccc aggtctcgtt
    26821 gaagacgatg cccaggtcgg cgcggccgtc gatgacggcc ttggcggcgt tgaggtggtt
    26881 gccggcgaac tcgaagcgcg ccgcggccgg gtcgatgccc agcgtgcgca gcacctgcag
    26941 gcccagatcg tggatgatca gcttgtcggt ggcactggcg acggtggggg cgtccggcac
    27001 cgcgccttcg gtccgggcga cgatgacggc ctcgtccacc acacccgccg gccgcgcgac
    27061 cggcacgaag cccttgtcgc gggcgaagca cagggcgctg taggggttgg catagaccac
    27121 gtggaaacgg gcgtcgagca cgcgctggcg ttcgacaagg aagttgtcct ggggctcgaa
    27181 gtggatgccg atgcccagct tgcgctggag gtaggtgttg agggtgaacc aaccgagcag
    27241 gtttttggcg gtatcgtggg ggcagacgct caactggaat tcgctggcca tgggtctctc
    27301 gtgggattgg gggtgaaacc tcgacgggcc ggaccgggcc ggcggcatcg aatcattttc
    27361 tgggacgata aaagtggaat ataacaattc caatcgcatt catgccacgc attctgtcgg
    27421 tgcccgtgac atccgtacgg cgggcggccc cggtttgtcc gggcgcggcg cgggcgggcg
    27481 cgcgcctttc gcctatctga cgcggctgcc gcccggcaag gcggtgctgt ggtgttacct
    27541 gatctggtac ctgtccacgg tggcccacca cttcgacccc tcgcccaagc tgtggctcaa
    27601 cgccctgggc atcagcggcg tgatcggcat tgccctgatg ctcagcgtgg ccggcacaac
    27661 ccgtccggac cgttggcaga cggcgcggct gttcatgatg cccttcggcg tgtcgagctt
    27721 ttcggcgttg atcaaggggc agggcttcgt gctgatcttc ccgccgacct gggccgaatg
    27781 cgcggtcagc gccggggcct gcgccttgtt cctcgcggtc gtggccgccg caaaggcgct
    27841 ggcgcggcag ccgggcacga cgcctcagta gccgccgatg cggcgcacga gggcgtattc
    27901 gcaggacttg aacaccaggt agaagaccat gctcagcacg gtgctgagca gcaggtcgag
    27961 cagttgaccg agggcgccat ccatggccgc catgctgccg tgttccagga acagggccag
    28021 gttggccttg agcagggtca ccagattcag cgacgcgatg ccgaaggcga cgaaggacag
    28081 cgcgagcacc acgaaggtga gccacgcgcg ggcgaagacg aattgacgaa cggtcttgag
    28141 catgtccggc tcctgggtgt ggggtctgat gtgcaccaga ccgggccgga ccgccgcgcc
    28201 ttacatccag tccaccagcg acacgccgaa accgatcacg ttctggcggt ggttgtagtc
    28261 gatcagggtc tcgccgtagc cgctgaacac ctgcaggtag cctttcagat tgcgatggat
    28321 cgggaagctc cagtccacgc cggccgagcc gcgcccggcg gtgggattgc cgcgcaggtt
    28381 gaggccgagg gtctgctgac tgaagcgcca cacggcctgc acgtcgccgt ggccgaggta
    28441 gtgggtgatg tcggcgttgt cgtcgttgcc cgcattttca tggaggcgca cccaggggcg
    28501 cacgatcagg gaaaaatcgc ccctttccac gccggcctgg gccatgaccc ggttccagct
    28561 gcgggacaga tcgcccgcac ggccgttgga ctggtggttg aggccgatgg agagcaggcg
    28621 cagacggaaa ccggcgccca ggtcccggtt ggtacggaac accgccatca gctcgggctc
    28681 gtagtcggtc tcgcggaagg gcgaggagtt gcccttgttg tagacctgcc agctggattg
    28741 ctgggtatag cccagccaca ggtcgccatg ccggccgaac aggccttccc acagcttggt
    28801 cttgaagctg agctgatact tcgcctccac cgaatccagc gtgccgctgg tggcagccgt
    28861 gcccaggcgc ggcgaggtgg gggtggggtt gcgggcgtcg ctccagcgca ccggcaggat
    28921 gtagctgggc ttgtaggggc ggaacaggaa ggtgccgtgc ttgtcgtcgg cctccagctc
    28981 ccagcgggat gacagggggt cgtcgcccca ttcccgccgg ggcaggccga aggcgtcctg
    29041 gggcgggggc tggtccgcgt cggccagggg cgcgctgacc gccgccgcgg ggacgggcgg
    29101 ag
//
LOCUS       DAOQPA010000035        28317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_758_length_28317_cov_15.021053, whole genome shotgun sequence.
ACCESSION   DAOQPA010000035 DAOQPA010000000
VERSION     DAOQPA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 28317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28317
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_28317_cov_15.021053"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..662)
                     /locus_tag="PKX01_06975"
     CDS             complement(129..662)
                     /locus_tag="PKX01_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ75905.1"
                     /translation="MMRRKHQGGATYLLTLFAVAALGVALARFGAVWTTTLQREREAE
                     LIFIGGEFARAMASYRSAGSGTEPEAPPELESLLLDPRFPYVRRHLRKLYRDPMTGKA
                     DWLIERQGGRIVGVRSRSTQHALRCAALPAWVRNVARVPEDARYSDWLFHPEERSGAR
                     NHGETASESGSLWPPSR"
     gene            complement(659..1045)
                     /locus_tag="PKX01_06980"
     CDS             complement(659..1045)
                     /locus_tag="PKX01_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ75906.1"
                     /translation="MTPRRPIPCKGFTLVELLVVMAIVAMLLSIAAPRYFGHLARARE
                     AALAETLAVTRDAIDKFNGDVGRYPRDLDELVTGRYLRALPLDPLTERRDDWILVAPP
                     DGGEGVSDLHSSAQGTARDGRAFNAL"
     gene            complement(1045..1515)
                     /locus_tag="PKX01_06985"
     CDS             complement(1045..1515)
                     /locus_tag="PKX01_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ75907.1"
                     /translation="MSGRGFTLIEMLVTVAVVAVLAAMAVPVGLVTVQRAKEGELRTA
                     LRQIREGIDTYKRYVDEGRIARAADANGYPPHLEDLVRGAPDAKSPGAARIYVMRRIP
                     RDPFNTDAAIAASETWGRRSYASPPDAPEEGRDVFDVYSRSPSIGLNGIPYRDW"
     gene            complement(1519..3798)
                     /locus_tag="PKX01_06990"
     CDS             complement(1519..3798)
                     /locus_tag="PKX01_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspD"
                     /protein_id="HMZ75908.1"
                     /translation="MNCSIIRRVLALVAALLLVVGCAANPALEESRKAFATGHPEAAL
                     RSLQDKVSADPKNLELRAYFLRQRDTLILRQVLAADQARIAGRFDEAERALAVARPFD
                     PRNPRVIAAAEAIGAQKQRKLQMEDAERRFSKGDLAGAEAAVRAVLAADPSHPVASAL
                     MRRLDERAAALDSAPLALAGPFSKLVSLEFRDAPLRGVFEALSRASGLNFVMDRDVRP
                     DIKVTLFVRDNSIDDVIKLLMVTQQLERKLLNGNSVLIYPNTPVKQKDYQELVTRSFF
                     LANADPKQAMAMIKQMVKTRDVFVEEKLNLLVMKDTPAAVRLAERLVADLDLAEPEVM
                     LDVEVLEVSRNTLLELGTRFPDQIGYGQLQPTTTSSVTTPTGTTLTQSLGGQLLQGNI
                     NLRQTGELVPFVANPGLLLNLKDQDGNSNILANPRIRVKNRDKAKIHIGEKLPVFTTT
                     STANVGVSSSVNYLDVGLKLEVEPSVHLDDEVAIKVNLEVSSIAKEVTGPAGSLAYQV
                     GTRSAITSLRLRDGETQVLAGLISDEERSSANRLPGLGELPGVGRLFSSQRDVGNKTE
                     IVLLITPRILRNIAKSGSASPTQAAGTESTVGALPLLIGATRAGALGLKSPAGAALPP
                     AVAQALVASAAAPTGVSASVEGVRPALRLEVPDEVHPGEEFAVTVTLESVAGEGCSLQ
                     MGFDSSLVDIVDGQSERGDVVVLQLPAGPASSLAVRFRARPGAVGRAGFSLLSAQGLT
                     PGQGQAVLLPAPGGVQIVP"
     gene            complement(3795..4238)
                     /locus_tag="PKX01_06995"
     CDS             complement(3795..4238)
                     /locus_tag="PKX01_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75909.1"
                     /translation="MLLVAWVAGLEDDDLPAPPSVRRVPPRPTARSVGSTALDVPPRR
                     SVAPDMRNSSGDLFPVVSFEPPPPPPPPSLKPVAPPLPFRYAGGLDERGDMTAFLADG
                     EQIRVVRAGELIDGRYRVVLISRVRIDFLYLPLNEPQSLATGNLP"
     gene            complement(4277..4744)
                     /locus_tag="PKX01_07000"
     CDS             complement(4277..4744)
                     /locus_tag="PKX01_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75910.1"
                     /translation="MLLLALAADGLVNRTLAAEAEALRQEARVVVSLRVRPLSPEESD
                     RQRLDRFYASFPDSAGLPDRLIRINGYALARQVAVDRAEYRSSKEPGTPLERVTLNVP
                     AHGDYPTLRAWLGDVLTDMPELAVESLSLKRSTIADPVLEAQVHLAIFLKSVP"
     gene            complement(4822..5265)
                     /locus_tag="PKX01_07005"
     CDS             complement(4822..5265)
                     /locus_tag="PKX01_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75911.1"
                     /translation="MLVLREHGQLDERVAGERARLAQFRHGVAKTASVPSPPTAPLSE
                     GEIRPAVAVARVLQRDWLALFGALETAMADPGVALLSVEQDAARGPLKLAGEARNLPE
                     VFALIHRLESGGVLRDVRLANYDFRSSGSVQVVGFVLGAHWEGGP"
     gene            complement(5379..>5908)
                     /locus_tag="PKX01_07010"
     CDS             complement(5379..>5908)
                     /locus_tag="PKX01_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75912.1"
                     /translation="LRIDWPAGLRAAERAAFTAHRFHSVFGDMGTWAILADQGAVGFP
                     SLACAIPAALVAAIDEAAQSRTLRVASLQPAYVSDFNRCRGRFRDDGAFARLETARIT
                     VGLWGDGRWRAFRSHPVELADAAAAGRCLTALLKTLPLEDLVPAGTLYTRGGDAVGPD
                     ALPAGWRGEAVEDAP"
     assembly_gap    5909..6008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6009..6250)
                     /locus_tag="PKX01_07015"
     CDS             complement(<6009..6250)
                     /locus_tag="PKX01_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75913.1"
                     /translation="MSPLWPESIRLHIGPDCLRLTRAGGAASESPLAADWAVGLATLP
                     VLPRRARLHVTVADRHARYLRIDWPAGLRAAERAAFT"
     gene            complement(6231..7898)
                     /locus_tag="PKX01_07020"
     CDS             complement(6231..7898)
                     /locus_tag="PKX01_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HMZ75914.1"
                     /translation="MPFSAAELAAAARRAGQDGVRLVEVLARPDGAAASDDFPARLAA
                     TVGLTCLTAAELFALAPDFDGLSFDEALRREALLGRDAGGHTVLAVADPFDTDLVDGL
                     GARLLHPFVVRLADRDDLTACLARHEESRRVVAGMLAGREEAATGDAGEDLSLRRIAG
                     DASPVVKLVNSTLYDAIKQGASDVHLESVPGGLVIKYRVDGVLARVGGVQGTELAEQA
                     VSRLKVMAELDIAERRVPQDGRFKVQVSGRPIDFRVSIMPSIHGEDAVLRILDKEHLT
                     RELAGLTLETLGFDPAVRQQLRHMAGEPYGMLLVTGPTGSGKTTSLYATLSETNTGHD
                     KIVTIEDPVEYQLPGVLQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDAETAEIA
                     VQAALTGHLVFTTVHANNVFDVIGRFMHMGIDPYNLVAALNGVVAQRLIRVNCPACST
                     ASEPDAALLRESGLATPADVQGFAFRAGSGCGQCRGTGYRGRKAIAEVLLLDDELRDL
                     IIARAPLRQLKDTARRRGFVSLREAGLQRVREGLTTLQELNRVTFVA"
     gene            complement(7910..9067)
                     /locus_tag="PKX01_07025"
     CDS             complement(7910..9067)
                     /locus_tag="PKX01_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMZ75915.1"
                     /translation="MGNGAAVQELEVEAVDEASARTLLAGRGLAVLSATALRGGGGRR
                     TGFPLMLFSQELLALLKAGIPLADGIAALAEKESRPAVQAVLTALGSGLREGRTFSAC
                     LESQPQAFPDLFVATLRAAERTSDLEPALARYIDYQRQLELVRSKLVSAAVYPALLLV
                     VGGAVMLFLLAFVVPRFAQVFETVGGELPWMSRVLLDWGRLVQENGALLGAAFLFAVC
                     AAVALVRRPETRAAAGRLLWRLPRIGERLRIYELARFYRTLGMLLRGGIPAVPAMGMV
                     SGLLSPGLRPGLMRAIAGVREGQAFAATLQAQGLATPVALRMLAVGERAGNLGEMLER
                     AAEFHEEESARWLDVLTRLIGPLLMLVIAVAVGLILIMLYLPIFQVAESIQ"
     gene            complement(9098..9556)
                     /gene="gspG"
                     /locus_tag="PKX01_07030"
     CDS             complement(9098..9556)
                     /gene="gspG"
                     /locus_tag="PKX01_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HMZ75916.1"
                     /translation="MNSTLPDASVRLGRRFFGGQAGFTLLELLVVMTIIGLLAGYVGP
                     KFFAHIGKSEVKAARAQIDGFEKALDQFRLDMGRYPSTEEGLAALMNKPAGDAGKWAG
                     PYLKKAVPNDPWGKPYQYVQPGEHGEFDLYSFGKDGQPGGTGEAADVVNW"
     gene            complement(9723..10181)
                     /locus_tag="PKX01_07035"
     CDS             complement(9723..10181)
                     /locus_tag="PKX01_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ75917.1"
                     /translation="MTKPAEFARPTQRLAPRAEQGPGAEPAQSVGLENPVPAFAPTAG
                     PARSPSPGGRSGEVGASFDAEYLDNPRPAYPAEARRRHLEGLVELWVEVGADGRPGEI
                     AVHRSSAVPDLDVAALDAVRRWRFVPARRGETPVAGEVIVPLRFSLSDAR"
     gene            complement(10440..12560)
                     /locus_tag="PKX01_07040"
     CDS             complement(10440..12560)
                     /locus_tag="PKX01_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ75918.1"
                     /translation="MTRVLPAVPVAGLMVVMFGAIPPVRAGEPPSLRVVEVTDSGASL
                     IGEAGSASQGAVSAEQLRNRPMLRPAEALETVPGLIVTQHSGDGKANQYFLRGFNLDH
                     GTDFATFVLGMPVNLPTHAHGQGYSDLNFLIPELVTGIHYRKGSYAAEQGDFSAAGSA
                     SFDYRRRVPAPELALTAGERGYRRLLATGSQDLGGGSLLAAFEAMGNGGPWAVPESLT
                     KTNLVLRYSQGTVDNGWAASLLAYDARWNATDQVASRAVNGGLIDRFGSLDATDGGKT
                     RRTALAAEWARTDAAGRTLAHVYAYEYALNLFSNFTYRLDYPATGDQFEQVDRRHAFG
                     GDWRHTFFHGGLGESSDSTLGVQLRDEHIGSVGLYRTRDRQRIDKLLQAYDENGNPTV
                     LTPVPATLREDRVHQTSVSLFLENETQWTGWLRAIVGLRSDHYRLDVGSLVPENAGLV
                     RSHLTSPKLTLVFGPWRGTEFYANYGMGFHSNDARGATATVDPTDRTTPVSRVNPLVR
                     TRGSELGIRSDVTPGLTSTLALWQLSIDSELLFVGDAGVTEASRPSRRSGVEWSNFWQ
                     LRPGVIADASLAWSRARFRDGDPAGSYIPDAISRTASLGVAAEQGRWSGGARLRAVGA
                     RPLMPDDSVRAPSSTLVNLRAGYRLAARWRMAVDVLNVFDRKVSDIDYYYASRLAGES
                     RAVNDLHTHPAEPRTVRLTLMASF"
     gene            complement(12571..13242)
                     /locus_tag="PKX01_07045"
     CDS             complement(12571..13242)
                     /locus_tag="PKX01_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75919.1"
                     /translation="MKHIAILMILGSSAVVQAAPIPVVFNSESYTTSAFASVPNGQDG
                     PYTDVASPPGMTQITSATASDAGGGAFASGIVDSRFLGASADVTHSSGDAFGSGIATY
                     EGRFDTAGGLLRLTLNFDAQVSGSAANLLNVVFVANGVTLFDDNFSLTGEYALDFLLP
                     QAASGVLDLTLSSTADSTAGSGFGLSSTFFALNSVPTPGGLALCLLGLGGLALNRGRR
                     VSSST"
     gene            complement(13255..14574)
                     /locus_tag="PKX01_07050"
     CDS             complement(13255..14574)
                     /locus_tag="PKX01_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HMZ75920.1"
                     /translation="MNPVRFVAHLVAASVLLLFAILAAAHPLGNNTVNRQARLDFTAD
                     RASVEYRLDLAELRTLAASEEADRDGDGTTSDAEWRAWRQRLGVELCRTLELRVDGQR
                     LPLRVEGARLRLQPGEAALPVLQADILLSAALPPAQDAGGASRRLSVSFDDHYREHDA
                     GWRELYVDARDRQRVAGAIATRDRSAGLTRFPDSERLVEGSARFVLTLPGVRQVPSRD
                     AALTRVSDAPVVDPAGPRAAAVGPDVPVPTGSISRAATADFLRLGMHHIATGWDHLVF
                     LLGLLLVSRRLGEILRVVTAFSLAHSATLMASAAGWVSVPGPWVEALIALTIAYVGAL
                     ALARRASGHGIWLAFLFGLVHGFGFAGALAETLGALETGGDVSWLWRLAAFNLGIEIV
                     QVALVLLALTVFRVVRQMPWRRKAHALASASVLASGLVWFVSRLSLL"
     gene            complement(14571..15668)
                     /locus_tag="PKX01_07055"
     CDS             complement(14571..15668)
                     /locus_tag="PKX01_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75921.1"
                     /translation="MNMQSIRTRRSGPLRSARGLAIALALVLPVATDAAPLDDKIALA
                     TRALDATRGSADPARYAAADKALAELSASAPDAPHVKVLRAWQQMARHRFQEALVLTD
                     AALNAIPADPLALALQADALTELGRYDEAVEAVQTLLDRSPAVVGFPRAAQLRFIRGD
                     LEGAAELTARALSRAEPGSRNARWLTRELARFEVLTGNVQGALSRLDAASVLEPEDHA
                     ARAHVLLIAGQTEAARMAWQRAFDAVPQAEYALPLWKLARARDDRREAGRLGRYLDAL
                     ARLDPEGLSNRTLIEYFGLGGRPAEALRLARAELRRRPDIYGHAQLAWTLNLAGLHAD
                     AREHGALALSTGCRDPELLRWLDDPATGSAR"
     gene            complement(15724..17292)
                     /locus_tag="PKX01_07060"
     CDS             complement(15724..17292)
                     /locus_tag="PKX01_07060"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HMZ75922.1"
                     /translation="MFRTRLNLALLSGLLTLAGIPAAQAASHREAPLVAQDPAADLTD
                     VYAFVSYDAENLARAPKDRKVTLIMNVNPGQNPADGPNYFNFADDVVYRLNVDNNRDG
                     VANDVVYEFRFQTEDRPVGGAGGLTSPVPMLGNPAIPAAFPLQGVTALNGPGSEGLTR
                     RQTYTVTEVRGGKSTVIFSGIAAVPSNSGSATFPDYESLAAQGIYSDAGSGIRVFVGQ
                     RAETFYIDLGGTFDTLNFRRSPPVQNGPDEDADTVNPFGVNRFANTNISTIALEVPIS
                     RLTADGKAASEANKLIGVYASTLRPKIRVLRETGGTANSGPLVQVARLANPLVNELII
                     NTPSKDLWNAQLPHTEAQFTQFYKSPVIAAALELSFGGFGIHVPASPRTDLMGILLKY
                     PGQPLAGQECGSPCSDLLRLDLSVPPTPADKQKRLGAALAGDPAGFPNGRRPNDDVTD
                     IVVRVTGGADYIGAKVGDGVNFLEGAPGAGTDDGPGYGRLSGNRLDVTTTGIAKEFPF
                     LPTPYPGRKGVGNY"
     gene            17738..18454
                     /locus_tag="PKX01_07065"
     CDS             17738..18454
                     /locus_tag="PKX01_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ75923.1"
                     /translation="MTETLQPSPALHGGQRQQGIGAEPEDEEPRAAECHDPLAPRGAA
                     HAAAGAGALEPLLKRIAHEDESALSALYDATCSRVYGLVLRILRDGAAAEEVTGDVFF
                     QVWRQALRYDSARGQALSWILTIARTRALDHLRRVDPAVRHPEPTLLLDAEPSTDTDP
                     QDILLACKDNALLHSALAALEPVQRQMIALVFFRGLTHEEVASHTATPLGTVKSHVRR
                     ALINLRSALAADLDRSIVQS"
     gene            18451..19275
                     /locus_tag="PKX01_07070"
     CDS             18451..19275
                     /locus_tag="PKX01_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ75924.1"
                     /translation="MNDKHTLDPDDPEGARDVQEKILESLTPLPLPPGQASAIKLRLF
                     ERIRASRDAGKDFINVRIEDGEWESLVPGVRVKRLCQRQRAVLLDLQPGAIIPFHRHH
                     EDEECVVLRGEAELGGITVREGDYHLASAGSRHGAVRSPSGALLYLRGTSIGHGLEVA
                     RDVLTALLPGLGQVPCTIPSNGGEWADYAPGVRVKILRDDGHSRSMLLRLEPGAAAER
                     HHHPLDEECLVMEGEVFIGDQLMRPGDYQLAPQGTDHPLVSSDIGSLLFVRGASAG"
     gene            19727..20815
                     /gene="moaA"
                     /locus_tag="PKX01_07075"
     CDS             19727..20815
                     /gene="moaA"
                     /locus_tag="PKX01_07075"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMZ75925.1"
                     /translation="MNQRVIPLADERQLARRPAIPSDRPTPDGRLADRRGRQVRDLRI
                     SVTDRCNFRCVYCMPKSVFDQDYAFLPRNALLSFEEITRIARLFTAHGVRKIRLTGGE
                     PLLRRNLEDLIAQLAALPDMEVTLTTNGVLLPRKARALKAAGLARVTVSLDALDDAIF
                     RAMNDVDVPVADVLAGIAAADAAGLGPIKINMVVKRGLNDSEILPMARHFRGSGHILR
                     FIEFMDVGSSNGWRMDDVVPSAEVIRRIGAEFPLEATDPNYTGEVAERWRYVDGGGEI
                     GVISSVTQAFCSTCTRARLSTEGRLYTCLFAQSGHDLRPLLREGCSDEELSAAIGAIW
                     QDRNDRYSELRTAATADLRKIEMSYIGG"
     gene            20821..21411
                     /gene="mobA"
                     /locus_tag="PKX01_07080"
     CDS             20821..21411
                     /gene="mobA"
                     /locus_tag="PKX01_07080"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HMZ75926.1"
                     /translation="MDVALITGLILAGGQGRRMGGCDKGLVHLDGKPLIAHILGRFAP
                     QVGPLLINANRNADAYAAFGHPVIADRLAGHAGPLAGLDAGLAACETPFLACTPCDSP
                     CLPEDLVARLADALESRGADAAVARAGGRLHPVFALIRCTTAEALTAYIDGGGRRVET
                     WFNGLHTVTCAFDECPAAFDNLNTLEDLRTHTHTPV"
     gene            21565..22488
                     /locus_tag="PKX01_07085"
     CDS             21565..22488
                     /locus_tag="PKX01_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75927.1"
                     /translation="MTTDLSDSDIPEGTKRIIDGRERVFYSGYWIKTYHVPADTLQAK
                     RQLIGALTRRLFNHVEHGLNVPGSRLNEARQSFEAETDPARRRVKGAMLAGALFNRAA
                     DIFTKLVELQSVGIEIQSDNALMRECGACLQEALGLGRLVLHISGEEGIDELWGEPFK
                     AFSIPLEDFYESRYIKIALTQRDIDRVAATVVECFDPLPCFHGIAAHMLEFANAAKIK
                     TETLRTDPDIFNIWTTFVTAAERLCGHFNDVLPNNDPDTERLVGDGRRLIQQGRDLIS
                     YITRARTPMPKSTTDFIERCKAYRARWDAHF"
     gene            22639..23790
                     /gene="ppk2"
                     /locus_tag="PKX01_07090"
     CDS             22639..23790
                     /gene="ppk2"
                     /locus_tag="PKX01_07090"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HMZ75928.1"
                     /translation="MNDKAAPATPRSNGKAPRPRRAAQGDTAPALNAQGIEAYAVDEA
                     VDAARDSKAAAVSDIVATLAKHDTASIAETLQAILAGASPDDAALLRKALQSEDDVIY
                     SKLPAHPDEELAEGWREGGYPYKNLMSRKAYEKQKYRLQVELLKLQAWVKETGQRVVI
                     LFEGRDAAGKGGTIKRFMEHLNPRGARVIALEKPSETERGQWYFQRYVQHLPTAGEIT
                     MFDRSWYNRAGVERVMGFCSHDEYLEFMRQTPEFERHLARSGVHLVKFWFSVSREEQR
                     RRFKERENHPLKQWKLSPIDLASLDKWDDYTKAKEAMFFHTDTADSPWTVIKSDCKKR
                     ARLNAMRYILHKLPYANKDLEHIGPLDPLLVGRAHVVYERGEHSGGPLI"
     gene            24058..25833
                     /locus_tag="PKX01_07095"
     CDS             24058..25833
                     /locus_tag="PKX01_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMZ75929.1"
                     /translation="MPPVLPSPPFALFDNNLDADGTSGAWLLTDLREEVVVRADGDWR
                     AALDRVDAAARAGAWVTLAATYELGEAIEPRLRRPAAAPGAPVLQAWIFARAEWLDDD
                     ACDAVLAALADEEPAACTPLRPTLDAPTYTRAIERIRRYIEEGDCYQVNFTFPLHGQL
                     IGSPAALYRQLRRAQPVRHGAYIVHRDGVILSRSPELFLERNGLCLASEPMKGTAPRS
                     SDPQRLASSAKDRAENVMIVDLLRNDLGRLVPPGGVRVEALCRLDAYPTVWQMSSRIV
                     AEPVHAGLGDIFSALFPCGSITGAPKIRAMEIIRTLEDGPRGLYCGALGWIRPGGDFR
                     FSVPIRTARVAPDGALRLDVGSGIVHDSRPDAEWQECHLKARFLTDLPTDLRLIETLR
                     LEPDGAHRVPFLTEHLERLSSSATALGFAFDATAFRRLLDGVQADSALRIRITLGRAG
                     DLTLETAPLTSGAPGPAPTICLAPDPVRSNDPRLRHKTTARQRYDRALARALADGHFD
                     TLFRNERGELTEGARSNLFILRNGELLTPPLACGLLNGVLRRRLIHTGEAREAVLTLD
                     DLRRADAVFMGNGLRGRVQVRLADT"
     gene            complement(26159..>26374)
                     /locus_tag="PKX01_07100"
     CDS             complement(26159..>26374)
                     /locus_tag="PKX01_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75930.1"
                     /translation="RVKVVRDSFSVPKSEHAQLKALREALAKEGRIATKSELLRVGIQ
                     LLAKEPLASLKTLVEGLSVVPKGKSAK"
     assembly_gap    26375..26474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26475..26922)
                     /locus_tag="PKX01_07105"
     CDS             complement(<26475..26922)
                     /locus_tag="PKX01_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75931.1"
                     /translation="MNGKTRSALRSTLKKEDAAVAARLPEVTAVAAETPAPVESVGLE
                     SAPAPVVEPTPSAGPAVEDVVPESAAVEADAPAAQAPARPKPVKASAKPKAAPKAAAS
                     SEAKADVKPAGVSSVEAKAVKVKAPRPTKEGKPEKAPKVAKAVKPVK"
     gene            complement(26922..27581)
                     /locus_tag="PKX01_07110"
     CDS             complement(26922..27581)
                     /locus_tag="PKX01_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ75932.1"
                     /translation="MARSRVVAVLSQKGGAGKSTLVMQLAGGLAKVGKKVAVGDLDPQ
                     ETALRWAESAPAEQPFPARVQSLAAAGDLPAAIRGCGKGCEVLLLDCPPSIESPATLA
                     ALQVADVALIPVVPSPTDLWSTRAVEKLVLHVQAGRKGLKARLVPNRVVRTNLAWDVL
                     EVMRDFALPVLGAALSQRNAYAQSAVIGGSVYQLGRAGAEAAREVNMLVAEVLKLTGE
                     R"
     gene            27760..28284
                     /locus_tag="PKX01_07115"
     CDS             27760..28284
                     /locus_tag="PKX01_07115"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HMZ75933.1"
                     /translation="MPCETRQLGVWQGDITTLAVDAIVNAANPTLLGGGGVDGAIHRA
                     AGPDLTAYCAGLGGCPTGEARLTPGFLLPARHIIHTVGPIWRGGRAGEAELLATCYRA
                     CFALAIAHDLHSLAFPAISCGVYGYPPEAAARIAVREARTALACAGGPTAVRLVAFDP
                     SMARLLHQALDAPA"
BASE COUNT         4865 a         9839 c         9108 g         4305 t
ORIGIN      
        1 aggagccaag acaagcaagc agaaaagcca atccgcgagg gttggctttt ttgttatcgg
       61 gtcgattgcg ggttcggagc cggcagaagc gggcagtgat cacccatctg ggcgcgaccg
      121 ctcagcggtc atcttgatgg gggccacaag gaaccgcttt ccgacgcagt ctccccatga
      181 tttcgggcgc cactcctctc ttccgggtga aagagccagt cggaatagcg cgcatcctcc
      241 ggaactctcg caacattgcg gacccaagcg ggcaaagcgg cgcaccgcag cgcatgctgc
      301 gtggagcggc tgcgcacgcc aacgatgcgc ccgccctgtc gctcgatcaa ccagtccgcc
      361 ttccctgtca tcggatccct gtacagcttg cgcaggtggc gccggacata gggaaagcgg
      421 ggatccagca gcaggctctc cagttccggc ggcgcctcgg gctcagtacc ggatcccgca
      481 ctgcgatagc tggccatcgc ccgagcaaac tcgccgccaa taaagatcag ttcggcctcc
      541 cgttcgcgct ggagcgtcgt cgtccacacg gccccaaaac gggcaagcgc cacacccagc
      601 gcagccaccg caaacagcgt gagcagatag gtggcgcccc cctgatgttt tcgccgcatc
      661 ataaggcgtt gaaggcccgt ccgtcacgag cggttccctg cgcgctgctg tgaagatccg
      721 atacgccttc cccgccatcc ggaggagcaa ccaatatcca gtcgtcccgg cgttcggtaa
      781 gcgggtcaag tggcagggcc cgcagatagc gtcccgtgac cagttcgtcg agatcacggg
      841 gataacgccc cacatccccg ttgaacttgt cgatggcgtc acgtgtaacg gccagggttt
      901 cggccagcgc agcctcccgg gcgcgtgcca gatgaccgaa atagcgcggc gccgcgatgg
      961 acagcagcat cgcaacgatg gccatcacca ccagcaattc caccagcgtg aagcccttgc
     1021 aaggaatggg tcgccgaggt gtcatcacca gtcccggtag ggaattccgt tgagtccgat
     1081 gctgggactc cgggaataca cgtcgaagac gtccctacct tcctcgggcg catcgggagg
     1141 actggcatag ctgcgccgac cccaggtttc tgatgccgcg atcgcggcat cggtattgaa
     1201 ggggtcacgc ggaatacgcc gcatcacgta gatccgggcg gcaccgggcg atttggcgtc
     1261 aggcgcgccc cggacaagat cttcgaggtg cggcggatag ccgttcgcat ccgccgcccg
     1321 ggcaatgcgc ccttcgtcga catagcgctt gtaggtgtcg atgccttccc ggatctggcg
     1381 cagggccgtg cgcagttcgc cttccttcgc gcgctggacg gtgacgagcc cgacgggcac
     1441 ggccatggca gcgagtaccg ccaccacggc tacggtgaca agcatctcga tcagggtaaa
     1501 gccacggccg ctcatggctc agggcacgat ctggacgccg ccgggtgccg gcagtaagac
     1561 agcctgcccc tgccccggcg tcagtccctg cgcggacaac agactgaagc cggctcgccc
     1621 cacggcgccg ggccgggccc ggaaacgaac cgcgagactg gacgccgggc ccgccgggag
     1681 ttgcaggacg acgacgtcac cacgctcgct ctgcccgtcg acgatgtcca cgagggagga
     1741 atcgaacccc atctggagtg agcagccctc cccggcaacg ctttcaagcg tcacggtcac
     1801 cgcaaactcc tcacccggat gcacctcgtc cggcacctcc agcctgagcg cgggccttac
     1861 gccttcaacg gacgcggaaa cccccgtcgg tgccgcagcc gaggccacca aggcctgggc
     1921 cactgcaggg ggcaacgctg cgcccgccgg agacttgagc cccagcgcgc ccgcccgggt
     1981 agccccgatc aacagcggca aggcccccac cgtcgattcc gtcccagccg cctgcgtcgg
     2041 gctggcagaa ccggatttgg caatattgcg caggatcctg ggcgtgatca gcagcacaat
     2101 ctcggtcttg ttgcccacat cccgctggct gctgaacagg cgcccgacac cgggcaattc
     2161 gccaaggccc ggcaggcgat tggcggaact gcgctcctcg tcgctgatga gcccggcaag
     2221 cacctgggtt tcaccgtcgc gcagacgtag ggacgtgatc gcactgcgcg tgcccacctg
     2281 ataggccagc gagccggccg gcccggtgac ttccttggca atactgctca cttccaggtt
     2341 caccttgatg gcgacctcgt catcaaggtg aaccgagggc tcgacttcca gcttcaaccc
     2401 gacgtccagg taattgacgg acgacgacac gccaacgttg gcggttgagg tcgtggtgaa
     2461 caccggcaac ttctcaccga tgtggatctt ggccttgtcc cggttcttca cccggatacg
     2521 gggattggcg agaatgttgg aattgccgtc ctggtccttg agattgagga gcagccccgg
     2581 gttggccacg aaaggcacaa gttcgcccgt ctggcgcaga ttgatattgc cctggagaag
     2641 ctgtccgccg agactctggg tgagcgtggt cccggtcgga gtggtgaccg agctggtggt
     2701 ggttggttgt aactggccgt aaccgatctg gtcaggaaag cgcgttccca gttccagcag
     2761 cgtgttgcga ctgacctcca gcacttccac gtccagcatc acttcgggct cggccaggtc
     2821 caggtctgca acgaggcgtt cggccaggcg cacggcggcc ggggtgtcct tcattacgag
     2881 caggttcagc ttctcctcta cgaagacatc ccttgtcttg accatttgct tgatcatcgc
     2941 catggcctgt ttggggtcgg cattggccaa aaagaagctg cgcgtcacca actcctgata
     3001 gtccttctgc ttgaccgggg tattcggata gatcagcacc gaattcccgt tgaggagctt
     3061 gcgttcaagc tgctgggtca ccatcagcag cttgatcaca tcgtcgatgc tgttgtcccg
     3121 cacgaagagg gtgaccttga tgtccggccg aacatccctg tccatgacga agttgaggcc
     3181 ggaggcgcgg gacagggcct cgaacacccc gcgcaagggg gcatcccgga actccaggct
     3241 gaccagcttg gagaagggcc cggcgagggc aagcggagca gagtccaggg ccgccgcacg
     3301 ctcgtcaaga cgccgcatca gggcactggc caccgggtgg gatggatcgg cggccagcac
     3361 cgcccgcacc gccgcctccg cgccggctaa atcccctttc gaaaagcgcc gctcggcgtc
     3421 ctccatctgc agcttgcgct gcttctgtgc gccaatggct tccgcagcgg caatcacccg
     3481 tggatttcgg ggatcaaacg ggcgggctac ggcgagggcg cgctcggcct cgtcgaagcg
     3541 cccggcgatg cgggcctgat cggcagcaag aacttgccgc agaatcagcg tgtcgcgctg
     3601 tcgcaggaag taggcacgca gttccagatt cttcggatcg gcgctcacct tgtcctgcag
     3661 gctcctcagg gccgcctccg gatggcctgt ggcaaaggcc ttgcggctct cctcaagtgc
     3721 ggggttggcc gcgcagccga ccaccaggag cagggcggcc acaagggcga ggacgcgccg
     3781 gatgattgag caattcatgg cagattcccg gtcgcaaggc tctggggttc attaagggga
     3841 agatagagaa agtcgatgcg gacacggctg atcagcacca cccgataacg tccgtcgatc
     3901 agctcgcccg ccctcacgac gcgtatctgc tcgccgtctg caagaaaggc cgtcatgtcc
     3961 ccccgctcgt ccagccctcc cgcatagcga aagggcaagg gcggagcgac cggcttgagg
     4021 gatggcggag gcggaggggg cggcggctcg aagctcacca ccggaaacag atcgcccgaa
     4081 ctgtttcgca tgtcaggcgc cacagaacgc cgtgggggca cgtcgagcgc ggtcgaaccg
     4141 acactgcgcg cggtcgggcg aggcggcaca cgacggaccg atggaggggc cggcaggtcg
     4201 tcgtcctcca gtccggccac ccaggcaacc agaagcatcg tcgcgaggag cccacccccg
     4261 atcagcccgc tccgcctcat ggcacactct tcagaaatat cgccagatga acctgagcct
     4321 ccagaaccgg atcggcaatg gtgctgcgct tgagggacag cgactcgacc gccagctctg
     4381 gcatgtcggt cagcacgtcg ccaagccagg cccgcaacgt cgggtaatcg ccatgcgcgg
     4441 ggacgttgag ggtgacacgc tccaagggcg taccgggctc cttgctggaa cggtactcgg
     4501 cccggtccac cgccacctgg cgggccaggg catagccgtt gatgcggatc agccgatcgg
     4561 gcaacccggc tgaatccgga aagctcgcgt agaaacggtc aaggcgctgc cgatcagact
     4621 cctccggtga cagcggacgg acccgcaggc tgaccacgac gcgtgcctcc tggcgcaacg
     4681 cctccgcttc ggcggccagc gtccgattga ccagcccatc cgcggcaagc gcgagcagca
     4741 gcaaccctgc cccggcccca cccgcccaac cggcgttgcg cagcgctcgc catgcggact
     4801 gccgaacgcg atcacgccat gtcacggccc cccctcccag tgcgcgccga gcacaaagcc
     4861 gacgacctgc accgatccac tggaccgaaa gtcatagttg gccaagcgta cgtcccgcag
     4921 cacgcccccc gactccagcc ggtgaatcag ggcaaagacc tcgggcagat tgcgagcctc
     4981 acccgccagc ttgagcggac cccgggccgc atcctgctcg acggacagca aggccacgcc
     5041 ggggtcggcc atcgcagttt ccagcgcacc gaaaagtgcc agccagtctc gctgcagcac
     5101 gcgagcgacg gccacagccg gccggatttc gccctcactc aggggcgccg tggggggaga
     5161 cgggacggat gcagtcttcg ccacgccgtg ccggaactgg gcaagccgag cacgctcccc
     5221 ggccacccgt tcatccagtt gcccgtgttc gcgcagcacg agcatcgtgg ccgccacacc
     5281 acaggccagc agtagcccgc caaacgcgct cggccgacgt ggccggcggg caaagtcgat
     5341 ccccaacggg ggtggcatgc gccagcgcgt tctggggctc acggcgcgtc ctccacggct
     5401 tcgccgcgcc agccagccgg cagtgcgtcc ggaccgacag cgtcaccgcc acgggtatag
     5461 agcgtgccgg ccggcacgag atcctcaagg gggagcgttt tcagtagcgc agtcagacag
     5521 cggcccgcag ccgccgcatc cgccagttcg accggatggc tgcggaacgc ccgccaccgg
     5581 ccgtcgcccc acagcccgac agtgatccgc gcggtctcca gacgggcgaa ggcaccgtcg
     5641 tcccgaaaac gaccgcggca gcggttgaag tcgctcacgt aggccggttg aagactggcc
     5701 acgcgcagcg tgcgggactg cgccgcttcg tcaatggccg ccaccagcgc cgcagggatg
     5761 gcgcaggcca ggctcggaaa gcccaccgct ccctggtcgg ccagaatggc ccaggtcccc
     5821 atatcaccga acaccgaatg aaagcgatgg gcggtgaagg ccgcccgctc cgccgcgcgc
     5881 aggccggccg gccagtcgat gcgcaggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnngt gaaggccgcc cgctccgccg cgcgcaggcc ggccggccag tcgatgcgca
     6061 ggtagcgggc atggcgatcg gccaccgtga cgtgcaggcg tgcgcgccgc ggcaacacgg
     6121 gcagcgtcgc caggcccacc gcccagtcgg cggcgagggg cgactcggat gcggcgcccc
     6181 cggcccgcgt caggcgcagg caatcgggcc caatgtggag ccgtatggac tcaggccaca
     6241 aaggtgacac ggttcagctc ctggagcgtg gtgaggccct cgcggacccg ttgcaggccg
     6301 gcctcgcgca ggctgacaaa gccgcgccgg cgggcggtgt ccttgagctg gcgcaggggc
     6361 gcccgggcga tgatgaggtc gcgcagttcg tcgtccagca ggagcacctc ggcgatggcc
     6421 ttgcgcccgc gatagccggt accgcggcac tggccgcagc cggatccggc acggaaggcg
     6481 aagccctgca cgtcggccgg ggttgccagg ccggattccc gcagcagggc cgcatcgggt
     6541 tcgctcgcgg tgctgcaggc cgggcagttc acgcggatca ggcgctgggc caccacgccg
     6601 ttgagggccg ccaccaggtt gtaggggtcg atgcccatgt gcatgaagcg cccgatcacg
     6661 tcgaacacgt tgttggcgtg caccgtggtg aacacgaggt ggccggtgag ggccgcctgc
     6721 acggcgatct cggcggtctc ggcgtcgcgg atctcgccca ccatgatctt gtccgggtcg
     6781 tggcgcagga tggagcgcag gccccgggcg aagctgaggc ctttcttttc gttcaccggg
     6841 atctgcagca cgccggggag ttggtattcc accgggtcct cgatggtgac gatcttgtcg
     6901 tggccggtgt tggtctcgga gagggtcgcg tacaggctgg tggtcttgcc ggaaccggtc
     6961 gggccggtga ccagcagcat gccgtagggt tcgccggcca tgtggcgcag ttgctggcgc
     7021 acggccggat cgaagccgag ggtctccagg gtgaggcctg ccagctcgcg ggtcaggtgt
     7081 tccttgtcga ggatgcgcag cacggcatct tcgccgtgga tgctgggcat gatggagacg
     7141 cggaagtcga tgggccgccc gctgacctgc accttgaacc ggccgtcctg gggcacgcgg
     7201 cgctcggcga tgtccagttc ggccatcacc ttgaggcgcg acacggcctg ctcggccagc
     7261 tccgtgccct gcacaccccc cacgcgggcg agcacgccgt ccacccggta tttgatgacc
     7321 agcccgccag gcacgctttc caggtggacg tcgctggcgc cctgcttgat ggcgtcgtag
     7381 agcgtggagt tgaccagctt gaccaccggg ctggcgtccc cggcgatgcg ccgcagggag
     7441 aggtcctcgc cggcgtcgcc agtggcggcc tcctcgcggc cggccagcat gccggccacc
     7501 acccgccggc tctcttcgtg gcgcgcgagg caggcggtca ggtcgtcccg gtctgccagg
     7561 cggacgacga agggatggag caggcgcgcg cccaggccgt ccaccagatc ggtatcgaag
     7621 gggtcggcca ccgccagcac cgtgtgaccg cccgcgtccc gcccgagcag ggcctcgcgg
     7681 cgcagggcct cgtcgaagct caatccgtcg aaatccggtg ccagcgcgaa cagctcggct
     7741 gccgtcaggc acgtcagccc gaccgtggcc gccaggcgtg ccgggaagtc atcgctcgcg
     7801 gctgcaccgt ccggtcgcgc cagcacctcc accaggcgca cgccgtcctg tccggcccgg
     7861 cgcgccgcgg cggccagttc ggcggcggag aagggcatcg ctgacgcgct cactggatgc
     7921 tctccgccac ttggaagatg ggcaggtaca gcatgatcag gatcaggccc accgccaccg
     7981 cgatgaccag catcagcagc gggccgatca ggcgggtcag cacgtccagc cagcgggcgc
     8041 tctcctcctc gtggaattcg gcggcccgtt cgagcatctc gcccaggttg ccggcgcgct
     8101 cgcccacggc cagcatgcgc agggccaccg gcgtggccag gccctgggcc tgcagcgtcg
     8161 cggcaaaggc ctgcccttcg cgcacgcctg cgatggcccg catcagaccg ggccgcagtc
     8221 ccggcgacag caggccgctc accatgccca tcgccggcac cgccgggatg ccgccacgca
     8281 ggagcatgcc cagggtccgg taaaagcgcg ccaactcata gatgcgcagg cgctcgccga
     8341 tgcgcggcag gcgccacagc aggcgtccgg ccgccgcgcg ggtctcgggc cggcgcacca
     8401 gcgccaccgc ggcacacacg gcaaacagga acgcggcgcc cagcagcgcg ccgttctcct
     8461 gcaccagccg gccccaatcc agcagcacgc gggacatcca cggcagttcg ccgccgacgg
     8521 tctcgaagac ctgggcgaaa cgcggcacca cgaaggccag caggaagagc atcaccgccc
     8581 caccgaccac cagcagcagc gccggataca ccgccgccga cacgagcttg ctgcgcacca
     8641 gctcgagctg gcgctgataa tcgatgtagc gggccagcgc cggctccagg tcgctggtgc
     8701 gctcggcggc ccgcagggtg gccacgaaga ggtcgggaaa ggcctggggc tggctttcga
     8761 ggcaggcgga aaaggtgcgc ccttcccgca atccggagcc cagcgcagtc agcacggcct
     8821 gcaccgcggg gcgcgattcc ttctcggcca gggcggcgat cccgtcggcg aggggaatgc
     8881 ccgccttcaa cagggccagc agctcctggc tgaagagcat cagtggaaaa cccgtccgcc
     8941 gcccgccgcc gccccgcagc gcggtggcgg acagcacggc gagaccgcgc ccggccagca
     9001 gcgtccgcgc ggacgcctcg tcgacggcct ccacctcgag ctcctgcacg gccgcgccgt
     9061 ttcccacggc cttgatccgg aaacgcatcg acgcggccta ccagttgacc acgtccgccg
     9121 cctcgccggt accgcccggc tggccgtcct tgccgaagga gtacaggtcg aactccccgt
     9181 gttcgcccgg ctgcacatat tggtagggct tgccccaggg gtcattgggc acggctttct
     9241 tgagataggg tccggcccac ttgccggcgt cgccggcggg cttgttcatc agcgcggcca
     9301 gtccctcctc ggtgctcggg tagcgtccca tgtcgaggcg gaactggtcg agggccttct
     9361 cgaagccgtc gatctgggcg cgggcggcct tgacctccga cttgccgatg tgggcgaaga
     9421 acttggggcc cacatagccc gccaggaggc cgatgatggt catcaccacc agcagttcca
     9481 gcagcgtgaa ccccgcctgc ccgccgaaga atcggcggcc aagccggaca cttgcatcgg
     9541 gaagcgtgct gttcatgctg gaaatgtccg cgtggtatcg gtgaagtctg gggtgcgccg
     9601 gaaaggcgga tgcggcaggc gacgcgcccc ttgccgagct atacgcgtgc accggcaatc
     9661 cggatgcacg cgacgtcgtt gagtcgccgc gtcacctatc ccgcccagac ggcaggcccg
     9721 cctcaacgcg cgtcggacag cgagaaacgc aggggcacaa tcacttcgcc cgccaccggg
     9781 gtttcacccc gccgcgccgg aacaaagcgc cagcgacgaa ccgcatcaag ggccgcgaca
     9841 tccagatcgg gcacggccga gctccgatga acggcgatct cgccaggacg accatcggcc
     9901 cccacctcca cccagagctc caccaggccc tcgaggtgac gtcggcgtgc ctcggcgggg
     9961 taggccggac gggggttgtc gaggtattcg gcgtcgaagg acgcaccgac ctccccgctt
    10021 cgcccacccg gagacggaga ccgggcgggg ccggcggtgg gcgcaaacgc cggcaccggg
    10081 ttctcgagcc ccacggactg ggcgggttcg gcgcccggcc cctgctccgc acggggggcc
    10141 agacgctgcg tcggcctggc gaactcggca ggtttcgtca tgaccgccag ccgtcccttc
    10201 gtgggtgagc tcggtaccat ctgtgtcggc acggcctgcg ccggccgtgg cgcaagcaca
    10261 gggcgcggag caggcccctg cagggaaacc cggatcggcg cggcaacagg cggcgcaggc
    10321 acggcgacaa aaccggaagt gccgacccag gcccccgcgg cgaggtggag catgagggaa
    10381 gctgcacaag ccgtcccaaa ctcacttccg atccgaggca tggtgctcat gatgccgcgc
    10441 tagaacgagg ccatcagggt gaggcgcacg gtccggggct cggccgggtg agtgtgaagg
    10501 tcattcaccg cacggctctc ccccgccagc cgtgacgcgt agtagtagtc gatgtccgac
    10561 accttgcggt cgaatacgtt gagcacgtcc accgccatcc gccaacgggc agccaacctg
    10621 tagccggcgc gcagattgac cagcgtggag gacggcgcgc gcacggaatc gtccggcatg
    10681 agtggacggg cgccaacggc tcgcaggcgc gccccgccgc tccaccggcc ctgctccgcc
    10741 gccacgccga gcgacgcggt gcgggaaatg gcgtctggta tgtaggagcc cgcggggtct
    10801 ccatcccgga agcgggcccg cgaccatgcc aggctagcgt cggcgatgac ccccgggcga
    10861 agctgccaga aattgctcca ttcgacgccg ctgcgtcggg acgggcggct ggcttcggtg
    10921 acaccggcat cgccgacaaa caggagttcg gagtcgatgg aaagctgcca cagcgccagc
    10981 gtgctggtaa gaccgggggt cacgtcgctg cgtatgccca gctcactgcc gcgggttcgc
    11041 accaagggat tcacgcggct caccggggtg gtgcgatcgg tgggatccac cgtcgccgtc
    11101 gccccccggg catcgttact gtggaagccc atgccgtagt tggcatagaa ctcggtcccg
    11161 cgccacgggc caaacaccag ggtcagcttg gggctggtca gatgcgaacg gaccagcccg
    11221 gcgttctcgg gcaccaagct gcccacgtcc aagcggtaat gatcgctgcg caggcccacg
    11281 atggcccgca gccagcctgt ccattgggtt tcgttctcga ggaataggga cacgctcgtc
    11341 tgatgcacgc gatcttcccg gagggtggcc ggaaccggcg tcaggacggt cgggtttccg
    11401 ttctcgtcat aggcctgcaa gagcttgtcg atccgttggc ggtcgcgggt ccgatacagc
    11461 ccgacgctgc cgatgtgctc gtcgcgcaat tgcacaccga gtgttgaatc cgaggattcc
    11521 cccagaccgc cgtgaaagaa ggtgtgccgc caatcccccc cgaaggcgtg acggcggtcg
    11581 acctgctcga actggtcacc cgtcgccgga tagtccagcc ggtaagtgaa gttggaaaag
    11641 agattgaggg cgtattcgta ggcatacaca tgggccaggg tccgcccggc ggcatccgta
    11701 cgggcccact ccgcggcaag tgcagtgcgg cgggtcttgc cgccatccgt ggcgtcgagg
    11761 ctgccgaagc ggtcgatcag ccccccattg accgcccggc tcgccacctg atccgtggcg
    11821 ttccagcggg cgtcataggc caggaggctg gcggcccagc cgttgtccac cgtgccctgg
    11881 ctgtaacgca ggaccagatt ggtcttcgtc aggctttcgg gcacggccca cggccccccg
    11941 ttgcccatgg cctcgaaggc ggccagcaga ctgccgcccc ccagatcctg ggagcccgtc
    12001 gcaagcaggc ggcgatagcc ccgctcgcca gcggtcaggg caagttccgg cgcgggcacg
    12061 cggcgccggt agtcgaagct ggcagagccg gcagcggaga agtcgccctg ctccgccgca
    12121 tagctgccct tgcggtaatg gataccggtc accagctcgg gaatcaggaa gttcaggtcc
    12181 gaatagccct ggccatgggc atgagtcggc agattcaccg gcatgccgag cacgaaggtg
    12241 gcaaagtcgg tgccatgatc cagattgaaa ccccgcagga agtactggtt ggccttgccg
    12301 tcgccgctat gctgagtcac gatcagccct ggcactgttt ccagggcttc agccggacgc
    12361 agcatcgggc ggttgcgcaa ttgctccgcg gagacagcgc cctgagatgc gctgccggct
    12421 tcaccgatca gacttgcccc ggaatccgtg acctccacca cgcgcagact gggaggctcc
    12481 cccgcccgca caggcgggat ggcaccgaac atcacgacca tcaggcctgc caccgggacg
    12541 gccggtaaaa cccgcgtcat ggaggcggcc tcaggtggag gaggacacgc ggcgcccccg
    12601 gttcagggcc aagccgccca agcccagcag gcagagggcc aacccgccgg gcgtgggcac
    12661 gctgttgagc gcaaagaacg tcgagctcaa gccgaagccg gagcctgccg tcgagtcggc
    12721 ggtgctgctg agggtcaggt cgaggacacc gctggccgcc tggggtagca ggaaatcaag
    12781 cgcgtactcg ccggtgaggc tgaagttatc gtcgaagagc gtgaccccat tggcgacaaa
    12841 gaccacgttc agcagattgg ctgcactgcc ggacacctgg gcgtcgaagt tcagcgtgag
    12901 ccgcaagaga ccgccagccg tatcgaagcg tccttcgtat gtggcgatgc cgctaccgaa
    12961 agcgtcgccg gaactgtggg tgacgtccgc actggcaccc aggaagcggc tgtccacgat
    13021 gccggaggcg aaggcaccgc cgccggcatc ggaggccgtc gcagaggtga tctgggtcat
    13081 ccccggcggc gaagccacgt cggtgtaagg accgtcctgc ccgttgggga cgctggcaaa
    13141 ggcgctggtg gtgtaggact cgctgttgaa gacaacggga atgggcgccg cctgaacgac
    13201 ggcggatgaa ccaaggatca tcaggatggc gatgtgtttc atgggcgtaa ctccttaaag
    13261 aagagacaaa cgggaaacga accagaccag accgctggca agtacggacg ccgacgccag
    13321 cgcatgggcc tttctgcgcc agggcatctg gcgcaccacg cggaacaccg tcagggccag
    13381 aagcaccagg gcaacctgaa cgatctcgat gcccaggttg aaggcggcca ggcgccataa
    13441 ccaggacaca tcgcccccgg tttcgagcgc cccgagggtt tctgccaggg cgccggcgaa
    13501 accgaagccg tgcacaagac caaacaggaa ggccagccag atgccatgcc cactcgccct
    13561 tcgggccagc gccagtgcgc ccacataggc gatagtcagc gcgatcaacg cctccaccca
    13621 cggacccggc accgacaccc agccggcggc ggaggccatc aaggttgcgc tgtgggccag
    13681 cgagaaggcc gtgaccacgc ggaggatctc gccaagtcgg cgcgacacaa gcagtagtcc
    13741 gagcaggaat acgaggtgat cccagccggt ggcgatgtgg tgcatgccga ggcgcaagaa
    13801 atccgccgta gcggccctgg agatcgaccc cgtcgggacc ggcacatcgg ggccgacagc
    13861 ggccgcacgc ggcccggccg gatcgacgac cggcgcgtcg gacacccgcg tcagcgccgc
    13921 gtccctggac gggacctggc ggacgcccgg cagggtgagc acgaagcggg cgctcccctc
    13981 gaccagtcgt tcagagtccg ggaagcgggt cagccccgca ctgcggtcgc gggtggcgat
    14041 cgcaccggcg acacgctggc gatcgcgggc atccacgtag agttcacgcc agccggcatc
    14101 atgctcccgg tagtggtcgt caaaactcac cgacagtcga cgggacgcgc ccccggcatc
    14161 ctgcgcaggg ggcaaggctg cagacaggag gatgtctgct tgcagaaccg gcagcgccgc
    14221 ctcgcccggt tgcagacgca agcgagcccc ctccacccgc aagggaagcc gctggccgtc
    14281 cacccgcaac tcgagggtac ggcacaattc cacgcccagg cgttggcgcc atgctcgcca
    14341 ctcggcgtcg gaggtcgttc cgtcgccgtc ccggtcggcc tcttcggagg ccgcaagggt
    14401 gcgtagctcc gcgaggtcga ggcggtactc gacgctggcc cggtccgccg taaaatccag
    14461 gcgtgcctgt cgattgacgg tgttgttgcc gaggggatgg gccgcagcca ggatggcgaa
    14521 caggaggagg accgaggcgg cgacgaggtg ggcaacgaag cggacggggt tcatcgcgca
    14581 gacccggtgg caggatcgtc cagccagcgc agcagttccg gatcgcggca gccggtactg
    14641 agcgcgaggg ctccgtgctc gcgcgcatcg gcgtgcaacc cagccagatt cagggtccag
    14701 gcaagctggg cgtgaccata gatgtcaggg cggcggcgca actcggcacg cgcaaggcgc
    14761 agggcctcgg caggccggcc gccgagaccg aaatactcga tgagtgtccg gttggaaagc
    14821 ccctccgggt cgaggcgcgc cagcgcatcg aggtagcgcc ccaggcgccc cgcttcccga
    14881 cggtcatccc gggccctcgc cagtttccac agcggcagcg catattcggc ctgaggtacg
    14941 gcatcaaagg cgcgctgcca ggccatccga gccgcttcgg tttgaccggc gatgaggaga
    15001 acgtgcgctc tggcggcatg atcttccggc tccagcacgg atgccgcgtc gagccgggac
    15061 agcgcgcctt ggacattgcc cgtcagcact tcaaaccggg ccagttcgcg ggtcagccag
    15121 cgggcattgc gacttcccgg ctcggcgcgg ctgagtgccc gggcggtgag ttcggcggcg
    15181 ccctccaggt cgcctcggat gaagcgcaac tgggccgcac gggggaatcc gaccaccgcc
    15241 gggctgcggt cgagcagcgt ctgtacggcc tccacggcct cgtcgtaccg ccccagttcc
    15301 gtgagggcat ctgcctgcaa ggccagtgcc agaggatcag ccggtatggc attcagcgca
    15361 gcatctgtga ggacaagggc ttcctgaaag cgatggcgtg ccatctgctg ccaggcccgc
    15421 agaaccttta catgcggcgc gtccggcgct gacgcggaca gttcagcgag tgccttgtcc
    15481 gcagcggcat aacgggccgg atcggccgag ccgcgcgtgg cgtcaagcgc ccgcgttgca
    15541 agggcgatct tgtcgtcgag tggggcggca tccgttgcga ccgggaggac gagcgcgagc
    15601 gcgatggcaa ggcctcgcgc cgagcgcagg ggcccgctgc gtcgggtccg gatggattgc
    15661 atgttcatgt cgggcggccg ggctgttttc cactgcgaaa caggcccggc cgatctccgc
    15721 ctatcagtag ttgcctacgc ccttgcgccc agggtaaggc gtcggcagga aggggaactc
    15781 cttggcaatg ccggtagtgg tcacgtccag cctgtttcca gacaggcgac cgtagccagg
    15841 accgtcatcg gtccctgctc cgggcgcgcc ctccaggaag ttcactccgt cgcccacctt
    15901 ggcaccgatg tagtctgccc cgccggtgac ccgcacgacg atgtcggtca cgtcgtcatt
    15961 cggacggcgg ccgttgggga agccggccgg gtcgccagcg agggcggcgc ccaggcgctt
    16021 ctgcttgtcg gcgggggtcg gcggcaccga gaggtcgagg cgcagcagat cggagcacgg
    16081 ggatccgcac tcctgcccgg ccaacggctg acccggatac ttcagcagga tgcccatcag
    16141 gtcggtacgg ggagatgcgg ggacgtggat gccgaagccg ccgaagctca gctccagcgc
    16201 ggcagcgatc accggcgact tgtagaactg ggtgaactgg gcttcggtgt ggggaagctg
    16261 ggcgttccac aggtccttgc taggggtgtt gatgatgagc tcgttcacca gcggattggc
    16321 cagccgcgcc acctgcacca gcggaccgct gttggccgtg ccaccggtct cgcgcagcac
    16381 ccgaattttc ggacgcaagg tcgaggcata caccccgatc agcttgttgg cctcgctggc
    16441 ggccttgccg tcagcggtca gacgagagat cgggacttcc agcgcaatgg tggagatatt
    16501 ggtgttggcg aagcggttga cgccaaaggg attgaccgtg tcggcatcct cgtccgggcc
    16561 gttctgcacc ggcggcgaac ggcggaagtt gagggtgtcg aaagtaccgc cgagatcgat
    16621 gtagaaggtt tccgcgcgct gccccacaaa tacgcggata ccgctgccgg cgtcgctgta
    16681 tatcccctgg gcggccaggc tctcgtaatc cgggaaagtg gcgcttccac tgttggaggg
    16741 caccgcagcg atgcccgaga agatcaccgt gctctttccg ccgcgcactt cggtcaccgt
    16801 gtaggtctgc cgacgggtca ggccctcgga gccaggacca ttgagcgcgg tgaccccctg
    16861 cagcggaaaa gccgccggaa tcgccggatt tccgagcatc ggaaccggcg aggtcaagcc
    16921 accggcgcca ccgacgggcc ggtcttcggt ctggaaacga aactcgtaca ccacgtcgtt
    16981 cgcgacgcca tcccgattgt tgtcgacatt gaggcgatac actacgtcgt cggcaaagtt
    17041 gaagtagttg ggaccgtcgg cggggttctg ccccggattg acgttcatga tcaacgtgac
    17101 cttgcggtcc ttcggcgcac gcgccaggtt ttcggcgtcg tagctgacaa aggcatagac
    17161 atcggtcagg tcggcagcgg gatcctgggc aaccagtggc gcctcgcggt ggctcgcggc
    17221 ctgcgcggcc ggaatgcccg cgagggtcag gagcccggac agtagggcaa gattgagtcg
    17281 ggtacggaac atcgtgattc tccttcgttc gagagcggcc gattgggggc aatgggcggc
    17341 gcactgagcg ccaccgtcag ccgctctacg caggagaaag cgatccggat gcagcgaccc
    17401 ggaagaaatc cgctcgccca tcctaacgat ccaagggaaa gcgggtgtcg ggcatatacc
    17461 tcaagactgg ttctgtccgg gtgcacggta tgcctcaaac acaagaattc ggacaggctc
    17521 tccgccctcg gccgccggtg cctccgctcg ccaactcacc cgtcacaaaa aattgatgca
    17581 tgttctgcat ccggaatcag ctggcctgcg tacagtccga agcgttcaag tgcattttcg
    17641 acactcgaac caatcacctt tgagtacctt gtgatggcca tacctgatcc ggacctacca
    17701 tgcgcccctg ctccaagcag aaataggagt gcttgccatg actgagaccc ttcaaccgtc
    17761 cccagccttg cacgggggcc aacgtcagca agggattgga gcggaacccg aagatgagga
    17821 gccgagggcc gcggaatgtc acgacccgct tgccccccgt ggcgcggccc atgccgcggc
    17881 gggcgccggc gccctcgagc ctcttctgaa acgcatcgcc cacgaagatg aatccgccct
    17941 gtcagccctt tacgatgcaa cgtgcagccg ggtctatggc ctggtgctgc gcatcctgcg
    18001 cgacggggcc gcggccgaag aggtaacggg agacgttttc ttccaggtct ggcgccaggc
    18061 attgcgctac gattccgccc gcgggcaagc cttgagctgg attttgacca ttgcccgcac
    18121 ccgggccctc gaccacctgc gccgggtaga tcctgccgtg cgtcatcccg agcccaccct
    18181 cttgctggat gccgaaccga gcaccgacac cgaccctcag gacatccttc tggcctgcaa
    18241 ggacaatgcc ctgctccatt cggccctggc tgcacttgag cccgtacaac gtcagatgat
    18301 tgccctcgtc ttttttcgcg gtctgaccca tgaggaggtg gccagccaca ccgcaacccc
    18361 cctgggtacg gtcaagtccc atgtccgccg cgccctgata aacctgcgca gcgcccttgc
    18421 cgcagatctg gatcggagca ttgtgcaatc atgaacgaca aacacaccct tgatcccgac
    18481 gaccctgaag gcgcccgcga tgtgcaggag aaaatcctgg aatccctgac cccgctgccg
    18541 ctgccgcccg gacaggccag cgccatcaaa ttgcgcctgt ttgaacgcat cagggccagc
    18601 cgcgacgccg gcaaggactt catcaacgtg cgcatcgagg acggcgagtg ggaaagcctg
    18661 gtcccgggcg tacgcgtcaa gcgcctctgc cagcgccagc gcgccgtact gctcgacctg
    18721 cagcccggcg caatcatccc cttccatcgc caccatgaag acgaagaatg cgtcgtcctg
    18781 cgcggcgagg ccgagctggg cggtatcacg gtcagggagg gcgattacca cctggccagc
    18841 gccggcagca ggcacggtgc agtgcgctcg ccttctggcg ccctgctcta cctgcgtggc
    18901 acatccatcg gccacggcct ggaagtggca cgagacgttc tgactgcgct cctgccaggc
    18961 ctcggacagg tcccctgcac catccccagc aatggcggag agtgggccga ttatgcgccg
    19021 ggcgttcgcg tcaagatcct ccgggacgac ggccacagca gatcaatgct gttgcgcctc
    19081 gaaccggggg ccgccgcgga acgccatcac caccccctcg atgaggagtg cctggtgatg
    19141 gaaggagagg tcttcatcgg cgatcagctc atgcgccctg gcgactatca gctcgcccct
    19201 cagggaaccg accatccgct ggtgagcagc gacatcggca gcctactgtt tgtgcggggg
    19261 gccagcgcag gatgaagtca tccatgatat tcacgaggcc gggcttgtgc cgagggtctg
    19321 atcgcttgaa agcccgcggt cggcccgggc tgaccgcagt agggcgagac tgacgggggc
    19381 agccgtcgta gacgcagacg cgttgacttt catgtcggga tctttccagt cgggaagcag
    19441 acacccggct gcatcaaccg cgccggccgt cacgcacggc gctctcaagg gctgatctgc
    19501 aggaccctgt ttcgagcccc ggtcgagacg ccaggacatg ggatcgggcc cgttccgtgg
    19561 aacgggcctt gtgcattccg gccgtctgat ctgaccgacc cgccttgcgt ttccccttcc
    19621 ctccgcccat gatcttcctg caagatcccg gttttgctcc aggataggcc gtgctaggcc
    19681 agaacgcgta tatcgaacac ctctgccagc ccaccgcgaa gaccccatga accagagagt
    19741 gattcccctc gccgacgagc gccagcttgc aaggcgcccc gccattccca gcgaccggcc
    19801 gactccggac ggacgtctgg ccgatcggcg cggccgacag gtgcgtgatc tgcgcatctc
    19861 ggtcaccgac cgctgcaatt tccggtgcgt gtactgcatg cccaagtcgg tgttcgatca
    19921 ggattacgcc ttcctgcccc gcaacgcgct gctgagtttc gaggaaatca cccgtatcgc
    19981 gcgcctcttc accgcccacg gtgtacgcaa gatccgtctc accggcggcg aaccgctgct
    20041 gcgacggaat ctggaggatc tgatcgccca gctggcggcg ctgcccgaca tggaggtcac
    20101 cctcaccacc aacggcgtgc tgctgccgcg caaggcgcgg gccctcaagg ccgcaggact
    20161 ggcgcgggtg acagtgagcc tggacgccct ggacgacgcc atcttccgcg cgatgaacga
    20221 cgtggatgtt ccggtcgccg atgtgctggc gggcatcgcc gcggccgacg cggccgggct
    20281 tggccccatc aagatcaaca tggtggtcaa gcgcggactc aacgacagcg agatcctgcc
    20341 catggcccgg cattttcgcg gcagcgggca catcctgcgt ttcatcgaat tcatggacgt
    20401 gggcagctcc aatggctggc gcatggacga cgtggtgccg tcagccgagg tcatccggcg
    20461 catcggggcc gagttcccgc tggaggcgac cgaccccaac tataccggtg aggtcgccga
    20521 gcgctggcgt tacgtggatg gcggcggcga gatcggcgtg atttcatccg tgacccaggc
    20581 cttctgttcc acctgtaccc gcgcccgcct gtccaccgag gggcgtctgt acacctgcct
    20641 gttcgcccag agcggccacg acctgcggcc cctgctgcgg gaaggctgca gcgacgagga
    20701 actgtctgcc gccatcggcg caatctggca ggaccggaac gatcgctact ccgaactgcg
    20761 caccgccgcc accgccgacc tgcgcaagat cgaaatgtcc tatatcggcg gctgagggcc
    20821 atggacgtcg ccctcatcac cggactcatc ctggcgggcg ggcagggacg ccgcatgggg
    20881 ggctgcgaca agggcctcgt gcacctggac ggcaaaccgc tgatcgccca catcctcggt
    20941 cgctttgcac cccaggtcgg cccgctgctc atcaacgcca atcgcaatgc cgatgcctac
    21001 gcggcgttcg gtcatccggt catcgccgat cgcctggcag gccatgccgg cccgctcgcc
    21061 gggctcgacg ccggcctggc ggcctgcgaa accccgttcc tggcgtgcac gccctgcgat
    21121 tcaccctgcc tccccgagga tctcgtggcc cgcctggccg acgcgctcga atcccgaggc
    21181 gctgacgccg ccgtggcgcg ggcgggcggg cgcctgcatc cggtctttgc gctgatccgc
    21241 tgcaccaccg ctgaggccct gacggcctac atcgacggtg gcggacggcg cgtcgaaacc
    21301 tggttcaacg gactgcacac cgtgacctgt gccttcgacg agtgtcccgc ggccttcgat
    21361 aatctcaaca cgctggaaga cctgcggacc cacacccaca cgcccgtctg acgcgcaggg
    21421 ctgccagaaa gtctgtcgta cgcccgccac accccggcgg gaatttgtat gtcgtggcac
    21481 gtcgctcatc aaaacttagg gcagaatcaa acgttcgagc ctcgatgaac gaccgcccca
    21541 cgacctctcc aacaagagcg gaacatgact accgatctct ccgacagcga catccccgaa
    21601 ggcaccaagc gcatcatcga cggtcgcgag cgggtgtttt actccgggta ctggatcaag
    21661 acctaccatg tcccagccga cacgctgcag gccaagcgcc agttgatcgg cgcgctgaca
    21721 cggcgtctgt tcaatcacgt ggagcacggt ctcaatgtac ccggcagccg gctcaacgag
    21781 gcccgccaga gcttcgaggc cgagaccgac ccggcacgcc gccgcgtcaa gggcgcgatg
    21841 ctcgccggcg cgttgttcaa ccgggcagcg gacatcttca ccaagcttgt cgaactgcaa
    21901 agcgtcggca tcgagatcca gtcggacaat gccctgatgc gtgaatgtgg cgcgtgtctg
    21961 caggaagccc tgggtctggg gcgactggtc ctgcacatca gcggcgagga aggcatcgac
    22021 gaactgtggg gcgaaccgtt caaggccttt tccatcccgc tcgaggactt ctacgagagc
    22081 cgctacatca agatcgccct gacccagcgt gacatcgatc gtgtcgccgc caccgtcgtc
    22141 gaatgtttcg acccgctgcc ctgtttccat ggcatcgccg cccacatgct tgaattcgcc
    22201 aatgcggcca agatcaagac ggaaaccctg cgaaccgatc cggacatctt caacatctgg
    22261 acaaccttcg tcacggccgc cgaacggctc tgcggtcatt tcaacgacgt cctgcccaac
    22321 aacgatccgg acaccgaacg gctggtcggt gacggccgtc gcctgatcca gcagggacgc
    22381 gacctgatca gctacatcac ccgtgcgcgg accccgatgc ccaagagcac gacggacttc
    22441 atcgaacgct gcaaggccta ccgggcccgc tgggacgccc acttctgaca ttctgtcatg
    22501 acataaagtc atatattgcc gtcatgaccg gccgcgcaga caattccgga tgcagcagcc
    22561 ccgcccgccg agacgcgttc agaaatgcgc ccgggcgctc catcccgaat cacgtcgcca
    22621 caagcagaaa ggttggccat gaacgacaaa gcggctcccg ccaccccccg gtccaatggc
    22681 aaggcgcccc gcccgcgccg cgcagcgcag ggcgacaccg caccggcgct gaatgcgcaa
    22741 ggcattgaag cctacgccgt cgatgaagcc gtcgacgcgg cccgtgactc caaggccgca
    22801 gccgtcagcg acatcgtggc caccctcgcc aagcacgaca ccgcgagcat cgccgagacg
    22861 ctccaggcga tcctggccgg cgcctcgccc gacgatgccg cgctcctgcg caaggcgctg
    22921 cagagcgaag acgatgtcat ctacagcaaa ctccccgccc acccggacga ggaactcgcc
    22981 gagggatggc gcgagggggg ctatccctac aagaatctca tgtcccgcaa ggcctacgag
    23041 aagcagaaat accgcctcca ggtagaactg ctcaagcttc aggcctgggt caaggagacc
    23101 ggccagcgtg tggtgatcct ctttgaaggt cgtgacgcag cgggcaaggg cggcaccatc
    23161 aagcgcttca tggaacacct caatccgcgg ggcgcccgcg tgatcgcact ggaaaagccc
    23221 tccgagaccg aacgtggtca gtggtacttc cagcgctatg ttcaacacct ccccactgcc
    23281 ggcgagatca ccatgttcga ccgctcctgg tacaaccggg ccggcgtgga gcgggtgatg
    23341 ggtttctgct cccacgacga gtatctggag ttcatgcgcc agacgccgga attcgaacgc
    23401 caccttgccc gcagcggcgt gcatctggtc aagttctggt tctcggtgag tcgcgaagag
    23461 cagcgccggc gcttcaagga gcgggaaaac caccccctca agcaatggaa actgagcccc
    23521 atcgacctgg cctccctcga caagtgggac gactatacga aggccaagga agccatgttc
    23581 ttccacaccg acacggccga ttcaccgtgg acggtcatca agtccgattg caagaagcgc
    23641 gcgcgcctca acgccatgcg ctacatcctt cacaagctgc cctatgccaa caaggatctg
    23701 gagcacatcg gccccctcga tccgctcctc gtgggccgcg cccacgtcgt atacgaacgc
    23761 ggcgagcact cgggtggacc gttgatctga gcggccccgc ggcaccacac ccgagggtgc
    23821 cgcggacgtt cccggacttt gcggacgcgt gcccggcgcg ccgtgggccg tggcctgaca
    23881 tcatccgatg cactcaggca ggcacgcgcg gcaggatccc gtcgtctgcg cgctgcgccc
    23941 gccggtacac acccacgcgt cgggtctgga cgcgagtgtc cctgcggcac cccgcatcag
    24001 gcgcccgtcc gtgaaaatgc gtccatccct ccggcgctgg cggtaaactc cgccgccgtg
    24061 ccccccgtcc tcccctctcc tcccttcgcc cttttcgaca acaacctgga tgccgacggt
    24121 acgtccggcg cctggctgct caccgacctg cgcgaggagg tcgtcgtccg cgccgacggt
    24181 gactggcggg ccgcactcga ccgtgtcgac gccgccgccc gcgccggcgc ctgggtcaca
    24241 cttgcagcca cgtatgaact gggcgaggcc atcgaacccc gtctgcgtcg gccggccgcg
    24301 gcccctggcg cccccgtgct ccaggcctgg atcttcgcca gggccgagtg gctggacgac
    24361 gacgcctgcg atgccgtcct cgccgcgctg gccgatgaag aaccggccgc ctgcacgccc
    24421 ctgcgcccga ccctcgacgc accgacctac acccgcgcga tcgagcgtat ccgccgctac
    24481 atcgaagaag gtgactgcta ccaggtcaat ttcacgtttc cgcttcatgg acagctgatc
    24541 ggctcgcccg ccgcgctcta ccggcagctg aggcgcgccc agccggtccg ccatggtgcc
    24601 tacatcgtcc atcgcgacgg agtcatcctg tcccgctcac cggagctgtt cctggaacgc
    24661 aatggcctgt gcctcgccag cgaacccatg aagggcaccg cgccgcgcag cagcgatccg
    24721 cagcggctgg cctcctcagc caaggaccgg gccgaaaacg tgatgatcgt ggacctcctg
    24781 cgcaatgatc tgggacgcct tgtgccaccg ggtggcgtac gggtcgaagc cctgtgccgc
    24841 ctcgacgcgt atcccaccgt ctggcagatg agctcgcgca tcgtcgccga acccgtgcac
    24901 gccggtctgg gggacatctt cagcgccctc ttcccctgcg gctcgatcac cggtgcgccc
    24961 aagatccgcg cgatggagat catccgtacg ctggaggatg gcccccgcgg cctgtactgc
    25021 ggcgcgctgg gctggatccg gccgggcggc gacttccgct tcagcgttcc gatccgcacc
    25081 gcccgcgtgg ccccggacgg cgccctgcgg ctggacgtgg gcagcggcat cgtccatgac
    25141 tcccgccccg acgcggagtg gcaggaatgc catctcaagg cgcgcttcct caccgacctg
    25201 cccacggatc ttcgcctgat cgaaaccctg cgcctcgaac cggacggggc tcaccgcgtc
    25261 ccctttctga ccgaacatct ggagcgcctg agcagctccg ccacggcgct gggcttcgcc
    25321 ttcgacgcga ccgccttccg ccgtctactg gacggcgtgc aggccgacag cgccctgcgc
    25381 atccggatca ccctcggccg cgccggcgat ctcacccttg agaccgcccc gctcacatcc
    25441 ggcgccccgg gccccgcccc caccatctgc ctcgcacccg accccgtgcg cagcaacgac
    25501 ccccgcctgc gccacaagac caccgcccgg cagcggtatg accgggcact cgcacgagcg
    25561 ctcgccgacg gtcatttcga caccctgttc cgcaacgaac ggggcgaact cacggaaggc
    25621 gcccgcagca acctgttcat cctgagaaac ggcgaactcc tcaccccgcc tctcgcctgc
    25681 ggcctgctca acggtgtcct gcgccgtcgt ctgatccaca ccggcgaggc ccgcgaggcg
    25741 gtgctcaccc tggacgacct gcgccgcgcc gacgctgtct tcatgggcaa tggtctgcgc
    25801 ggacgggtgc aggtacgtct ggcggacacc tgacaccacc gcgcatccag accgcgggcg
    25861 cggaccgccc ctgccgtcca cgcccccaga gccgtaccga cgcatcccaa cgctctggac
    25921 gatcgcccgg ccggcgggcc ctgcgcgcgt cactgcgtcg gagatcccca accaggcacc
    25981 tgcgcgcccg ctcaagcgca tatcgccaga cgattcagcc cgccacacct gcagcggatt
    26041 ccagaccggg cgcccggatc cggcgcgcgg tatcaccgag gatccggaag gcagcgcaac
    26101 gaaaaaaggg cgccgcagcg cccttttttc cacaccggat ggcggtatcc cgccgcgctt
    26161 acttggcgct cttgcccttg ggcacgacgc tgagaccttc caccagcgtc ttcagcgacg
    26221 ccagcggctc cttggccagg agctggatgc ccacccgcag cagttcggac ttggtcgcaa
    26281 tccgcccttc cttggccagc gcttcacgca aggccttgag ctgggcgtgt tcggacttgg
    26341 gcaccgagaa cgagtcccgc accaccttga cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnccttca cgggcttcac cgccttggcg accttgggtg ccttctcggg
    26521 cttgccctcc ttggtgggcc ggggtgcctt caccttgacc gccttggcct cgaccgacga
    26581 cacgccggcc ggcttgacgt ccgcctttgc ctcggacgag gcggccgcct tcggcgcagc
    26641 cttgggcttc gcagacgctt tcaccggctt cggccgggcc ggcgcctgcg cggccggcgc
    26701 atccgcctcg accgccgcag attcgggcac cacgtcctcg acggccggcc ccgccgacgg
    26761 ggtcggttcg accacgggcg ccggcgcgga ttcaagcccc accgattcga ccggcgccgg
    26821 cgtttcggca gcgactgccg tcacctcggg caggcgggcc gccaccgcgg cgtcctcctt
    26881 cttgagcgtc gagcgcagcg cagagcgggt cttgccattc atcatcgttc tcctgtcagt
    26941 ttcagtactt cagccaccag catgttcacc tcgcgtgccg cctcggcccc ggcccgcccg
    27001 agctgataca ccgagccgcc gatcaccgcg ctctgcgcgt aggcattccg ttgggacagg
    27061 gccgcgccga gcaccggcaa cgcgaagtca cgcatcacct ccagcacgtc ccaagccaga
    27121 ttggtccgca ccacccggtt gggcaccagg cgggccttga gccccttgcg ccccgcctgc
    27181 acatgcagga cgagcttttc caccgcccgc gtcgaccaca ggtcggtcgg actgggcacc
    27241 accggaatca gggccacatc ggccacctgc agggctgcca gggtggccgg gctctcgatg
    27301 gagggcggac agtcgagcag caacacctcg cagcccttgc cacaccctcg aattgccgcc
    27361 ggcagatcgc cggccgccgc cagggactgg acccgcgccg ggaagggctg ctccgcgggg
    27421 gccgactcgg cccagcgcag ggcggtttcc tgaggatcca gatccccgac cgcgaccttc
    27481 ttgccgactt ttgccagccc tccggccagt tgcatcacca gcgtcgattt gccggcaccg
    27541 cctttctggg acagcaccgc gaccacccgg ctgcgcgcca tgcgacctcc gtttgcgtaa
    27601 taccgtatga ttttatacca ctgaccgcaa ttcgcaagcg cccgccctgt ggttcgctca
    27661 ccgcacgggg cgccgcaccg cccgcattct gccgtccatc gcctgccgcg ggcgcagccg
    27721 gcacaggcat aatcgatgct ctgtcccgtg gaggccacca tgccctgcga aacccgtcag
    27781 ctcggcgtct ggcaaggtga catcaccacc ctcgcggtgg atgccatcgt caatgcagcc
    27841 aatccgaccc tgctcggcgg cggcggagtg gacggtgcca tccatcgggc ggccggcccg
    27901 gatctcacgg cctactgcgc cgggctcggc ggctgtccga ccggcgaagc gcgcctgacg
    27961 cccggcttcc tcctgcccgc gcgccacatc atccacaccg tcggtccgat ctggcgcggc
    28021 ggccgggccg gcgaggcgga gctactcgcc acctgctatc gcgcctgctt tgcgctggcg
    28081 atcgcccatg acctgcacag cctcgccttt ccggcgatca gctgcggcgt gtatggctac
    28141 ccgcccgagg ctgccgcccg catcgcggtg cgcgaggccc ggacggcact tgcgtgcgca
    28201 ggtggtccga ccgccgtgcg cctggtcgcc ttcgaccctt ccatggccag actcctgcac
    28261 caggccctgg acgcccctgc ctgacccgcc tgcgcgggct gggtatactc cccacac
//
LOCUS       DAOQPA010000036        26247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_858_length_26247_cov_18.975527, whole genome shotgun sequence.
ACCESSION   DAOQPA010000036 DAOQPA010000000
VERSION     DAOQPA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 26247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26247
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_26247_cov_18.975527"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKX01_07120"
     CDS             <1..402
                     /locus_tag="PKX01_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75934.1"
                     /translation="IAANADPAYYDLGLCGPSRSDLAARTDLCGAFRVPSLRNVAATA
                     PYFHNGSLKTLTDVVRFYVRRDTNPEEWYPTDSAGVVQKFNDLPEAYRRNVNTTEVPY
                     NRKPGDSPALDASEIADLVAFLNTLTDGYTP"
     gene            441..1778
                     /locus_tag="PKX01_07125"
     CDS             441..1778
                     /locus_tag="PKX01_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ75935.1"
                     /translation="MKTTPLSLALAACFLSSGAQAATDAELLARIDRLAAELEAVKAE
                     LRSVKAQATPPAPITTTAATTPAATVPVPAAPGAYAQTASASGANPTSLFGYGEVNYN
                     RPFRNSSDTRMDVRRAVIGVTHQASERTRIVGEFEFEHAVASATDSGEVEVEQLYVEH
                     RLNDNVGLKGGLFLIPLGTLNLSHEPLSYYGVERNFVETAIIPTTWREVGLSTFGVTD
                     EGLRWDVGLTSGFDLNKWDKTSDEGRTEGPLAAIHQEGSQARGHDLSVYAALDWRGTP
                     GLAVGGGVFSGKASHKVNDPSTANAGVYAPGARVTLWDTHARWTPGKWDLAALYARGS
                     ISDTDQLNVQLAGGTPVPKSFFGWYTQAAYRLWENGEYKLSPFVRHERFNTGATYAAM
                     PAGLEIDPLRTEKVLTAGANFQVGGNVVLKADVQRFVVDRSRDRLNLGLGYQF"
     gene            1863..2435
                     /locus_tag="PKX01_07130"
     CDS             1863..2435
                     /locus_tag="PKX01_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HMZ75936.1"
                     /translation="MKHSRHEWPVVPASLLIAAGALATFQAPHAIAAQYMSAEQAQKT
                     AFPDADRFDVRPLRLTPEQLRTLDGVAPVRQRGELRQILAYAGPRLLGTLYVDDVIGK
                     VEWITYALAINPDGSVRSLDILEYRETHGFEVRTASWRKQFVGHRSDTPLHFGDDIKN
                     ISGATLSCAHLTAGVQRLLKLHAQLASAER"
     gene            2489..3355
                     /locus_tag="PKX01_07135"
     CDS             2489..3355
                     /locus_tag="PKX01_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HMZ75937.1"
                     /translation="MEATAAEQRVADAAVAAAFDAVADVHRLMSFHAQDSDLANLHAD
                     AWRQPVTVSPHTWQVILHAQTVAAASDGVFDPTVAPALVASGALPAPTGPRPDPTATW
                     ADVELLDGQQIAFRRPLWLDLGGIAKGYAVDRAVHILCEYGADSGLVNAGGDLRVFGR
                     HRTRVPVAVRHPAQPSQTLPLGSLAQGAVAGSGDYFAAMPAEQASPIVAPGRGLRPAG
                     RTAVVVVADECWCADALTKVVSLMESASSPVLRMFNATAALLDAHGTLRAMPGFMERL
                     GHATPEEQSSHA"
     gene            3348..3863
                     /locus_tag="PKX01_07140"
     CDS             3348..3863
                     /locus_tag="PKX01_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75938.1"
                     /translation="MHEFPHRPRHKAVRPGRRQRHSLYAVAAVLMVSGLVWLVAHYAL
                     PLPDDAARHPAEPWAMRIHGAATLLGMAVLGALWSHHVLPAWRAGEKRATGLPLLGIW
                     LALAVSGYGLYYLAEESWRDAADTLHVSAGLAMPLALASHVWMQGRRQKKGERPSSGP
                     HPLSPQSKETG"
     gene            complement(3933..6080)
                     /locus_tag="PKX01_07145"
     CDS             complement(3933..6080)
                     /locus_tag="PKX01_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ75939.1"
                     /translation="MIVLFVSALLGLAVAGCDGVWNDPYPVAERGQNILYSAFTQRPK
                     HLDPVQSYSEDEATFLYQIYEPPLQYHYLKRPYQLEPATAATLPVLRQYDRAGHELPP
                     DADPARVARSTYEIRIRPGVRYQPHPAFAVDAAGQARYVALSRSDIAQRRRPSDFPES
                     GTRELTADDFVYQIKRLAHPRLHSPVLELMGDYIVGLKDLNARLVAEERGRADKGAWI
                     DLRRYDLPGVEVVDRYTYRVHLQGTYPQFPYWLAMPFFAPVPFEADRFFAQPGMADNN
                     LTLDSWPVGTGPYMLVENNPNARMVLARNPNFRGERYPCEGEPDDRAAGFLEDCGKVI
                     PFVDTVVFTREKESIPYWNKFLQGYYDASGVSSDNFDQAVQMGAQGDVALSDEMARKG
                     IRLLTSVAPTVMYMGFNMLDPLVGGDSERARKLRLAISIAIDQEEFISVFQNGRGVPA
                     MHPIPPGITGYRVDQAGINPYVYDWVDGKARRKSVDDARRLLAEAGYPNGRDAKTGEP
                     LVVNLDTTGSGMGDKSTVDWLTKQLNKLDVQLVVRATDYNRFQDKIRKGNAQLFYWGW
                     NADYPDPENFLFLLYGPQSKVKNQGENAANYANPEYDRLFERMKAMPDSPERLALIAR
                     MLAILQHDAPWVWGFYEKAYTLQHGWVSNRKPGKIVRSTLKYQRIDVTTRSAMRAEWN
                     RPVVWPLAVLLILLVVLALPAVAHYRRREQGNE"
     gene            6189..6983
                     /gene="fabI"
                     /locus_tag="PKX01_07150"
     CDS             6189..6983
                     /gene="fabI"
                     /locus_tag="PKX01_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HMZ75940.1"
                     /translation="MGFLAGKRILITGLLSNRSISYGIAKAMRREGAELAFTYQNERF
                     KERVAKLAAEFDSTLIFPCDVQNDAEIASLFEQLGTQWDGLDGLVHSIAFAPGEALEG
                     DFLDGLTREAYRVAHEVSAYSFPALAKAARPMMKGRKGSLLTLSYLGAVRTMPNYNIM
                     GLAKASLEASVRYMAVSMGPEGTRVNGISAGPIKTLAASGIGSFGKLLAFNEHNAPLR
                     RNVTIEEVGNAAAFMCSDLASGITGEIMYVDGGFNTTALGNAQPMA"
     gene            complement(7095..8996)
                     /locus_tag="PKX01_07155"
     CDS             complement(7095..8996)
                     /locus_tag="PKX01_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HMZ75941.1"
                     /translation="MFDAVRNNKKVVQIFLLLITLPFAFWGVDSYVRNMGKGDELASV
                     GGHAITQAEFQQAMRSQQDRLRASLGDQFNPAMLDSPEARRAVLENMINQRLLGLDAG
                     KHRLLVSDAQMRDTILAVPGFQENGQFSQVRYEALLKAQGMNQAIFESRLRQDLALQQ
                     IIIPLTQGILSGASAERVLGTQIEERTVSEAVLAPPQFMASVKLGEDAAKNFYEANRQ
                     RFEVPAQVRAEYVALDQDAVASQVSVSDAEVRKAYETAADRYRQGEERRASHILIQAD
                     KAAGEAVIKAAREKAEGILKQVKAAPGEFAKLAKANSQDPGSADKGGDLGYFGHGMMV
                     KPFEDAVFALAKEGDTTDLVQSDFGFHIIRLTGIKAAKVRPLEEVKGEIAAELRKKAA
                     SGKLAEMAEGFSNTVYEQSDSLKPAAEKYKLNVQTSDWIVRAAKLPPPFDNERLVSAL
                     FSDDAVKNKRNTDAIDVGGGVLVSARVVEYKPASLRPFEDVKPLIEKRLAADEASRLA
                     VKEGEARLGKLQKGEAVELSWGAPRVLQRGAQDLPPAAAAAVFRVASDKLPGYTGVEV
                     PGAGYVLYRVEKVVRPEAAASDPRLQVVRQQYGRVLAEMDFAAYIAALRQRYDVKINM
                     AALEGNKEK"
     gene            complement(9349..9425)
                     /locus_tag="PKX01_07160"
     tRNA            complement(9349..9425)
                     /locus_tag="PKX01_07160"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9389..9391),aa:Asp,seq:gtc)
     gene            complement(9454..9529)
                     /locus_tag="PKX01_07165"
     tRNA            complement(9454..9529)
                     /locus_tag="PKX01_07165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9494..9496),aa:Val,seq:tac)
     gene            complement(9564..9836)
                     /locus_tag="PKX01_07170"
     CDS             complement(9564..9836)
                     /locus_tag="PKX01_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ75942.1"
                     /translation="MNKSELIDQVSAEADISKAAVGRVLDATFAAVKLALKKGGSVTL
                     VGFGTFHVGKRAARTGRNPRTGAAIKIKSAKIPKFRPGKALKDALN"
     gene            complement(10295..10564)
                     /gene="rpsT"
                     /locus_tag="PKX01_07175"
     CDS             complement(10295..10564)
                     /gene="rpsT"
                     /locus_tag="PKX01_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMZ75943.1"
                     /translation="MANSAQARKRARQAIKARAHNMSLRSRLRTAIKAVRKAIDGGDK
                     SAAQQVFVASQRTIDSVADKKIIHKNKAARHKSRLSAAVKAMAAA"
     gene            10705..12243
                     /gene="murJ"
                     /locus_tag="PKX01_07180"
     CDS             10705..12243
                     /gene="murJ"
                     /locus_tag="PKX01_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HMZ75944.1"
                     /translation="MNLLRALATVSGMTLLSRILGFIRDFVVARAFGAGLATDAFFVA
                     FRLPNLLRRMFAEGAFSQAFVPILAEYKNRRGPDETRTLVDHVASALGVVVLIVSILG
                     ALGAPAIIYATAPGFSSDAEKFDLTVQLTRITFPYIFFMALVALSGGILNTWSRFAIP
                     AFTPVLLNLSFIGMALFAAPYFDPPVVALGWAVFIGGLAQVLLQIRPLARIGMLPRFS
                     LSFADPGVRRILKLMAPAILGVSVSQISLLINTVFASFLPSGSVSWLYYADRLMEFPA
                     GLLGVALGTILLPSLSKMHADDNRSEFSALLDWGLRLTLMLTLPSALALALLAVPLVT
                     TLFFHGAFKVADVYATRSALMAYSAGLAGLILVKVLAPAFYARQDIRTPVRIALITLA
                     ATQLMNLAFVGVFRHAGLALSIGLASCINAGLLFRGLRLRGIYLPQPGWRAFFLKLLA
                     ALTVLGGGLWLTMGADELWLTASGLDRVTRLTALVIGGIIIYFATLFALGFRVSDFHR
                     RGAT"
     gene            12303..12932
                     /locus_tag="PKX01_07185"
     CDS             12303..12932
                     /locus_tag="PKX01_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMZ75945.1"
                     /translation="MHLRSSSFADGAVISGVHTFCVPAAEGHVCLGANRNPHLAWREA
                     PAGTRSFVVLCHDPDVPSRGDDVNQEGRLVPADLPRVDFFHWVLVDLPETQMEIAEGS
                     HSSEVTPNGKPGPDRPDGSRHGLNDYTGWFAGDAQMKGQYFGYDGPCPPWNDSIRHRY
                     VFTVYALDVHRLTVTGAFTGQDVRNAMHGHILAQASLTVTYSLNPAIPA"
     gene            complement(12966..14216)
                     /locus_tag="PKX01_07190"
     CDS             complement(12966..14216)
                     /locus_tag="PKX01_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMZ75946.1"
                     /translation="MNGNLLYAQSGGVTAVINASAAGVITAARRVPQQIARVLAARHG
                     IMGVLQERLVDTSALTDADLACLGRTPGGAFGSCRLDLDPVERNPAQYDRLFAVLAAH
                     DVRALLYNGGNGSMETCRQIAAAAEERRFPLTVVGVPKTVDNDILLTDCCPGFGSAAK
                     YLATSVREVGLDLAAMTTGGGRAFVLEVMGRNAGWLAAACALASDGEGAAPHVILLPE
                     VPFDEEAFLAKVAEVVARHGACAVVVAEGLHDAAGEPIAMQERSAKGYVQLGGAGEAI
                     AERIHRRLGCKVHFAVADYLQRAARHLASATDAMQAGAVGEAAVAAALRGEGGMVAIE
                     RLGDVPYRWRTRIVPFDAVANLERRLPPEFIRPDGFGVTSAFRSWCRPLIEGEDWPAF
                     RDGLPEYFHLRLPLLTQRLPIWAA"
     gene            complement(14493..15146)
                     /gene="adk"
                     /locus_tag="PKX01_07195"
     CDS             complement(14493..15146)
                     /gene="adk"
                     /locus_tag="PKX01_07195"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMZ75947.1"
                     /translation="MRLILLGPPGAGKGTQANYIKEKFGIPQISTGDMLRAAVKAGTP
                     LGIEAKKVMDAGGLVSDDIIIGLVKDRLKEADCQAGYMFDGFPRTIPQAEAMKAAGVP
                     IDFVLEIDVPDEEIIGRMSGRRVHVASGRTYHVMFNPPKVDGKDDETGEALIQRDDDK
                     EETVRKRLDVYHSQTKPLVAYYSEWAASGDAAAPQYRKIGGLGKVEEIRDRAFGALK"
     gene            complement(15194..15967)
                     /gene="kdsB"
                     /locus_tag="PKX01_07200"
     CDS             complement(15194..15967)
                     /gene="kdsB"
                     /locus_tag="PKX01_07200"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMZ75948.1"
                     /translation="MGFRIVIPARYASSRLPGKPLLDIAGRPMILRVLDQARLAGADE
                     VWVATDHSDIAERVEAAGGQVILTGRELPSGTDRLAEVATRLGWDDDTVVVNVQGDEP
                     LIPPSLIADAAATLSADAEAAIATACHPLASAEEFFNPNVVKLVLDARGRALYFSRAP
                     IPWARDAFAQQRDALPAGFPAYRHVGLYAYRAGFLKRYAALASSPLEQWEALEQLRAM
                     AHGFPIRVMVLDHAPPGGVDTAEDLERVRRVFDVASVSR"
     gene            complement(15971..16159)
                     /locus_tag="PKX01_07205"
     CDS             complement(15971..16159)
                     /locus_tag="PKX01_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HMZ75949.1"
                     /translation="MDPRLLEILVCPITKGPLDYRKDKQELWSVSARLAYPIRDGIPV
                     MLEEEARPLTDDEVAQVR"
     gene            complement(16140..17150)
                     /gene="lpxK"
                     /locus_tag="PKX01_07210"
     CDS             complement(16140..17150)
                     /gene="lpxK"
                     /locus_tag="PKX01_07210"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMZ75950.1"
                     /translation="MPRRAPAFWSRRGAVAWALMPLSLLFGAVAGLRRGLFRNGWLRT
                     ERLPAPVIVVGNIAVGGSGKTPVTLWLVEHLKAAGRRPGIVSRGYGGHVEGVAEVPPD
                     GDPAHFGDEPVLMAHLAGVPVWVGRDRPAAGRALLAAHPEVDVLVCDDGLQHYRLERD
                     VEVVVVDQATLGNGLRLPAGPLREGVTRLARADLVLAHGALAPSVRRACGTTPVFEFH
                     LAGAAFERLSDRGERRPPSALMGRRLRALAGIGRPERFFAQLEAMGLAVERCPLPDHH
                     VFTPADVALEGVDALVMTEKDAVKCAPFAPHESWVWPVRAHVPSRAAELILEKIDGSP
                     SA"
     gene            complement(17185..17607)
                     /locus_tag="PKX01_07215"
     CDS             complement(17185..17607)
                     /locus_tag="PKX01_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ75951.1"
                     /translation="MNFRRGQSREEPEINLIPMIDVLLVIIIFLMLTTTYSRFAGLEI
                     NLPTADAQASAEKTVELNVAVTATGDVVVNKRAVSGTSVQAIAAALQALVPQGAKDPV
                     VIINADAKATHQRVIDVMQAAQQAGLVHISFATQAAQR"
     gene            complement(17617..18222)
                     /locus_tag="PKX01_07220"
     CDS             complement(17617..18222)
                     /locus_tag="PKX01_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ75952.1"
                     /translation="MFAILQAAGWPIWPLLFASIVAVALIIERLITLRRSKIIPDGLL
                     DKVVAELRQTGATPEMISRVALSSPLGRVFAAGLRNVRSPREVMKESIEEAGRAVTHD
                     LERFLTTLGTIASISPLMGLFGTVVGMIEIFGSQAPSGSNPQQLAHGISIALYNTGFG
                     LVIAIPSMIFWRHFRATVDSLVIEMEQQAIRLVEVVHGERR"
     gene            18522..19883
                     /gene="xseA"
                     /locus_tag="PKX01_07225"
     CDS             18522..19883
                     /gene="xseA"
                     /locus_tag="PKX01_07225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMZ75953.1"
                     /translation="MNPPENHPATPDSRVISVADLNRRAREVLEGTFPLQWIAGEVSS
                     LTRAASGHVYFTLKDDEAQVRCTLWRNRAQLLPFRLEHGMQVEIRAVVTLYEARGDFQ
                     LNVDTVRRAGQGNLYEAFLRLKARLEAEGLFASERKRPLPTYPRGIAVVTSPQAAAWQ
                     DVLAALARRAPHVPICLYPTAVQGSDAGARIAAALRLAGTRAVQDSTDVLLLVRGGGS
                     LEDLAAFNDEAVARAIRDCPLPVVCGVGHETDVTIADFAADLRAATPTAAAELASAGF
                     HALHERLSLLAGRLNRAMERRLTHAAQRLDRTALRLRHPRERLREAALRLDHLARRLQ
                     TAGHEPPAHGRNRLDALRLRLSARRPDASRARDRLESLAGRLQRALRIQLERRRARVD
                     ALTEHLTHLDPRSVLARGYTITRDARGRVLRNAADAVPGQDIRVEFHDGEADARVMAR
                     RLR"
     gene            19986..20573
                     /locus_tag="PKX01_07230"
     CDS             19986..20573
                     /locus_tag="PKX01_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HMZ75954.1"
                     /translation="MAHTLPELPYAKNALAPAISEETLEFHYGKHHQAYVTNLNNLIP
                     GTEYESLSLEEIVKKAPAGGIYNNSAQVWNHTFFWNSMKPNGGGAPSGALADAINAKW
                     GSFDDFKKAFQASAVGNFGSGWTWLVKKADGSVDIVNMGAAGTPLTTGDTALLCIDVW
                     EHAYYIDYRNLRPKFVETFLASLANWDFAAANFAA"
     gene            complement(20661..20894)
                     /locus_tag="PKX01_07235"
     CDS             complement(20661..20894)
                     /locus_tag="PKX01_07235"
                     /inference="COORDINATES: protein motif:HMM:NF023513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HMZ75955.1"
                     /translation="MNVVLNNPVLYVVDYPAHDAVEVIDKRRGVGVMFRDEAARRFRQ
                     ELLDLEATPHEMDDYEDWIDGYETLMTQPAVYH"
     gene            21063..21284
                     /locus_tag="PKX01_07240"
     CDS             21063..21284
                     /locus_tag="PKX01_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HMZ75956.1"
                     /translation="MNDCDEPDDEVTSLSARLAALRTEHRDLDAVIGQLISAPINDEL
                     LLRRLKKRKLLLKDRIAIFERILDPDVPA"
     gene            21342..22643
                     /locus_tag="PKX01_07245"
     CDS             21342..22643
                     /locus_tag="PKX01_07245"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HMZ75957.1"
                     /translation="MTLHRPAGADTLALHAGQTPDAPHGARAVPIHFSTSFVFSDVDQ
                     AAALFNLEQPGHVYSRLSNPTNAVLEERIAALDGGVGALACASGQAALHLAITTLMGA
                     GGHIVASRALYGGSHNLLAYTLPRFGIATTFVDPRDPDAWRAAVRPETRLLFGESLGN
                     PGLDVLDIPTVAQIAHDARVPLLVDATLVTPALQCPIDQGADLVLHSATKFLGGHGVA
                     IGGLLVDGGCFDWESAGRFPTLTEPYEGFHGMDFAEESPITAFLLRARREGLRDFGAC
                     LSPMNAFQILQGIETLGLRMERHVANAREIVQRLLGHPQVENIAWPELDGHPDQALAR
                     RLLPKGCGGVVSFRLKGGRPAGRAFIGALRLFSHLANVGDAKSLALHPASTTHFRMTP
                     EALAAAGIGEGTIRLSVGLESVSDLLDDVDRGLHAAQKATG"
     gene            22793..23626
                     /locus_tag="PKX01_07250"
     CDS             22793..23626
                     /locus_tag="PKX01_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ75958.1"
                     /translation="MDLRVTLSPNAPSECPVFAYTGGRSPAPDRPWIVFLHGAGNDHS
                     VWMLQTRAFAARGWNVLAPDLPGHGRSGGQALDTIEALADWTLRLLDAAAIERCALVG
                     HSMGSLIALHLASIAPERVFRLALVGSVFPMPVAPALLDKARIDPPAAHALINQWSFA
                     PATLLGRSAVPGIHPAAVNERLLQRQPVGTLPVALQACNAYAGGFEAAARIHCPTLLL
                     SAAQDRMTPPKALAPLADAFTSSAMPQRVVIPGAGHAMMNEAPTAVLEALERFMTPPA
                     G"
     gene            23683..24486
                     /locus_tag="PKX01_07255"
     CDS             23683..24486
                     /locus_tag="PKX01_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HMZ75959.1"
                     /translation="MAHSNLPTDDEARAAELPFPTLREVGFAAPFAWVGRGLADLKAN
                     PTASLFYGFCFAGMGLLVTFIFEHAYQYTSAATTGFLLMAPFFAMGLYELSRRRELGQ
                     PVQLGPTLTVWRRNAGNIAVFAAVLTVIFLIWARASLVVFAIFYTRELPNLSGFLTQV
                     LSFENIEFLAVYFAVGMIFALLVFAVSVVSIPLMLDRNQDAVTSMIASIRALALNPGP
                     LLVWAVLIVALTILGFLSFHLGLVVTMPIVGHATWHAYRDLVAPLPRHG"
     gene            24581..25288
                     /locus_tag="PKX01_07260"
     CDS             24581..25288
                     /locus_tag="PKX01_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990055.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMZ75960.1"
                     /translation="MGLKHLTACLAILTMNLTAARAADAPPTVTAPLADLSAEASRSA
                     PNDQLRALVYAEASDANAADVARRVNGLIAQALATARGYPAVKVKSAGNSTTPVYGKS
                     GRAIDAWRMRSSLSLESGDTAALSELLGKLQQSLAVASLNAAPSPETWRKVEDEVIGD
                     ALSAFQNRARLVAGGLGRGWRIRQISVNTSGGRPQPVMPYARAAMAIAEAAPAPVEAG
                     DTPITVTVTGQIELLDR"
     gene            25339..25776
                     /gene="dtd"
                     /locus_tag="PKX01_07265"
     CDS             25339..25776
                     /gene="dtd"
                     /locus_tag="PKX01_07265"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMZ75961.1"
                     /translation="MIAVIQRVLEARVTVDGRIVGAIGPGLLALVAVEKGDDSPRTAR
                     MAERLMSYRVFADEAGRMNRSLAETAGGLLLVSQFTLAADTARGTRPSFTPAAAPEDA
                     RRLFDELVRHARVRHPDVATGEFAADMKVALINDGPVTFRLTT"
     gene            25745..>26247
                     /locus_tag="PKX01_07270"
     CDS             25745..>26247
                     /locus_tag="PKX01_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ75962.1"
                     /translation="MAPSLFDSRPELRTALAAACVAAVLAGCGSAPPRTTSQPPPSVI
                     APPERAWFSLPHPDHAQEVVLYALGLLDTGYRFGGRNPEAGLDCSGMVSFIVEQVSGQ
                     RLPHNAAQIAERTRPVASGALQPGDLVFFNTLGRAYSHMGIYLGDGRFIHAPNSRGKV
                     RVDRLDNR"
BASE COUNT         4549 a         9449 c         7807 g         4442 t
ORIGIN      
        1 atcgccgcca acgcggatcc ggcctactac gacctgggcc tgtgcggccc gagccgcagc
       61 gacctggctg cccgcaccga cctgtgcggc gccttccgcg tccccagcct gcgcaacgtg
      121 gccgccaccg ccccctactt tcacaacggc agcctcaaga ccctcaccga cgtggtgcgc
      181 ttctatgtcc gccgcgacac gaatcccgag gagtggtacc ccaccgacag cgccggcgtc
      241 gtccagaagt tcaacgacct gcccgaggct taccgccgca acgtgaacac caccgaagtg
      301 ccctacaacc gcaagcccgg cgacagtccc gccctcgacg ccagcgagat cgccgacctg
      361 gtggccttcc tcaacaccct caccgacggc tacacgccct gagccgccgc atcgcttccc
      421 tgcatcccgg agatcctccc atgaagacca caccgctcag cctcgcgctc gccgcctgct
      481 tcctcagcag cggcgctcag gccgccaccg acgccgaact cctcgcccgc atcgaccgcc
      541 tcgccgccga actggaggcc gtcaaggccg aactgcgcag cgtcaaggcc caggccaccc
      601 cgcctgcgcc catcaccacg acagcggcca ccaccccagc cgccactgtg ccggtccccg
      661 cggctcccgg tgcgtacgcc cagaccgcca gcgccagcgg cgccaacccg accagcctct
      721 tcggctatgg cgaggtcaat tacaatcgcc ccttccgcaa cagcagcgac acccgcatgg
      781 acgtgcgccg cgccgtcatc ggtgtcaccc accaggcctc cgaacgcacc cgcatcgtcg
      841 gcgaattcga gtttgagcac gccgtcgcct cggcaaccga ttccggcgag gtggaagtcg
      901 agcagctcta cgtggaacac cgcctcaacg acaacgtggg cctcaagggc ggcctcttcc
      961 tgatccccct gggcaccctc aacctgagcc acgagcccct gtcctactac ggtgtggaac
     1021 gcaactttgt cgagaccgcc atcatcccca ccacctggcg cgaagtcggc ctctccacct
     1081 tcggcgtcac cgacgaaggc ctgcgctggg acgtgggcct caccagcggc tttgacctga
     1141 acaagtggga caagaccagt gacgaaggcc gcaccgaagg ccccctggcg gccatccacc
     1201 aggaaggctc ccaggcccga ggccatgacc tctccgtgta cgctgccctc gactggcgcg
     1261 gcacgcccgg cctggccgtc ggcggcggcg tcttcagcgg caaagcctcc cacaaggtca
     1321 acgacccttc caccgccaac gccggtgtat acgcccctgg tgcccgtgta accctgtggg
     1381 acacccacgc ccgctggacg cccggcaagt gggatctggc cgccctctat gcccgcggca
     1441 gcatcagcga caccgatcag ctcaacgtcc agctcgccgg cggcaccccc gtgcccaagt
     1501 ccttcttcgg ctggtacacc caggccgcct atcgcctgtg ggaaaacggc gaatacaagc
     1561 tgagtccctt cgtccgtcac gaacgcttca acaccggcgc cacctacgca gcgatgcccg
     1621 ccggcctgga gatcgacccg ctgcgcaccg agaaggtcct cacagccggc gccaacttcc
     1681 aggtgggtgg caatgtggtc ctcaaggccg acgtccagcg cttcgtcgtc gatcgcagcc
     1741 gcgaccgcct caacctgggc cttggctacc agttctgacc tggcctgccg gcacccccgg
     1801 acgcccggac gccgcttcgg cggcgcccgg gcgccgtcac gttcacccaa gggcttgaac
     1861 cgatgaaaca ttcccgacat gagtggccgg tcgtcccggc gtccctactg atcgccgccg
     1921 gtgcgctcgc caccttccag gccccccacg ccatcgccgc ccagtacatg agcgcggaac
     1981 aggcccagaa gacggccttc cccgacgccg accgcttcga cgtacgcccg ctgcgcctca
     2041 ccccggagca gctgcgcacc ctcgacggcg tggccccggt ccgccaacgc ggtgaattgc
     2101 gccagatcct cgcctacgcc ggtccccgcc tgctgggcac gctgtacgtg gacgatgtca
     2161 tcggcaaggt ggagtggatc acctacgctc tggccatcaa tccggacggc agcgtgcgca
     2221 gtctggacat cctcgaatat cgcgagaccc acggtttcga agtgcgcacc gcgtcctggc
     2281 gcaagcagtt cgtcgggcac cgcagcgaca cgccgctgca tttcggcgac gacatcaaga
     2341 acatcagcgg tgccaccctg tcctgcgccc acctcaccgc gggtgtgcag cgcctgctca
     2401 agctgcacgc ccagttggcc agcgccgaac gatgagcgac ggcccgaccg cacgccgggc
     2461 ccaggcctgg ctcggaacct atgtcgtcat ggaagcgaca gccgccgagc agcgtgtcgc
     2521 cgacgcggcg gttgcagccg ccttcgacgc tgtcgccgac gttcaccgcc tcatgagctt
     2581 ccacgcccag gacagtgatc tggccaacct gcacgccgac gcctggcgcc agcccgtgac
     2641 cgtctcgccc cacacctggc aggtcatcct ccacgcccag accgtcgccg ccgccagcga
     2701 cggcgtcttc gaccccaccg tcgcgcctgc cctggtggcc agcggtgcct tgccggcccc
     2761 caccggtccg cgtccggacc cgaccgccac ctgggccgac gtcgaactgc tcgatggcca
     2821 gcagatcgcc ttccgtcgcc ccctgtggct ggatctgggc ggcatcgcaa aaggctacgc
     2881 ggtggaccgg gccgtacaca tcctgtgcga gtacggtgcc gacagcggtc tcgtcaatgc
     2941 gggcggcgac ctgcgcgtgt tcggacggca tcgcacgcgc gtcccggtgg ccgtccgcca
     3001 ccccgcccag ccgtcccaga ccctgcccct cggcagcctc gcccagggtg cggtggccgg
     3061 ctccggcgac tactttgccg cgatgccggc ggaacaggcc tcccccatcg tcgcgccggg
     3121 ccgaggcctc cgtcccgctg gtcgcaccgc cgtggtggtg gtcgcggatg aatgctggtg
     3181 cgccgatgcc ctgaccaagg tggtaagcct gatggagagc gcctccagcc cggtgctgcg
     3241 catgttcaac gccactgcag ccctcctcga tgcgcatggt acgctgcgcg ccatgccggg
     3301 cttcatggaa cgcctcgggc atgccacccc tgaggaacag tccagccatg catgagtttc
     3361 cccaccgccc ccgccacaag gcggtccgcc cgggccggcg gcaacgccac agcctgtatg
     3421 ccgtcgcggc cgtcctgatg gtcagtggtc tggtgtggct cgtcgcccat tacgcgctgc
     3481 cgctcccgga tgatgctgcg cgccatccgg ccgaaccctg ggccatgcgg atccacggcg
     3541 ccgcaacgct gctgggcatg gccgtactgg gggccttatg gagccatcac gtgctgccgg
     3601 cctggcgggc gggcgaaaag cgcgcgaccg gactgcctct gctcggaatc tggctggccc
     3661 tggccgtcag cggctatggc ctgtattacc tggcggagga atcctggcgg gatgctgccg
     3721 acacgctgca cgtgagcgca ggactggcga tgcccctggc cctggccagc catgtctgga
     3781 tgcagggccg acgacaaaaa aagggggagc ggccttcttc aggaccgcac cccctgtcac
     3841 cgcaaagcaa ggagacaggc tgatcgcacc cgcaacgatg cgacgcctgc gtcatatatt
     3901 agccccttgc ccgtcgaatc gccacccgat tgtcattcat tgccctgttc ccggcggcga
     3961 taatgcgcca ccgccggcaa cgcaaggact accagcagga tgagcagcac ggccagcggc
     4021 cagaccacgg gtcggttcca ttccgcccgc atcgctgatc gcgtggtcac gtcaatgcgc
     4081 tggtacttga gcgtgctgcg cacaatcttg cccggcttgc ggttgctcac ccagccgtgc
     4141 tggagcgtgt aggccttttc gtagaagccc cacacccacg gcgcgtcgtg ctgcaggatc
     4201 gccagcatgc gcgcgatgag ggccagccgt tccgggctgt cgggcatggc cttcatgcgc
     4261 tcgaacaggc ggtcgtattc cggattggcg tagttggccg cgttctcgcc ctggttcttc
     4321 accttgctct gggggccata gagcaggaac aggaagttct ccggatcggg gtaatccgcg
     4381 ttccagcccc agtagaagag ctgcgcgttg cccttgcgga tcttgtcctg gaaacggttg
     4441 tagtcggtgg cccgcaccac gagctgcaca tcgagcttgt tcagctgctt ggtcagccag
     4501 tccaccgtgg atttgtcgcc catgcccgac ccggtggtat ccagattcac caccagtggc
     4561 tcgccggtct ttgcgtcgcg cccattgggg tagcccgcct cagcaagcag acgccgggcg
     4621 tcgtctaccg atttgcgccg cgccttgccg tcgacccagt cgtacacgta gggattgatg
     4681 cccgcctgat ccacccggta gcccgtgatg cccggcggga tcgggtgcat cgcgggtaca
     4741 ccgcggccat tctggaagac cgagatgaac tcctcctggt caatggcaat ggaaatcgcc
     4801 agccgcagct tgcgtgcccg ctccgaatcg ccgcccacca gcgggtcgag catgttgaag
     4861 cccatgtaca tcaccgtcgg cgccaccgaa gtcagcagac ggatgccctt cctggccatc
     4921 tcgtcgctga gggccacatc gccctgggca cccatctgca cggcctgatc gaagttgtcg
     4981 gaggacacgc ctgaagcgtc gtaatagccc tgcaggaact tgttccagta cggaatgctt
     5041 tccttctccc gggtgaagac caccgtgtcg acgaagggga tcaccttgcc gcaatcctcg
     5101 agaaagcctg ccgcacgatc atccggttca ccctcgcacg gatagcgttc accacggaaa
     5161 ttcgggttgc gggccagcac catgcgggca ttgggattgt tctcgaccag catgtacggt
     5221 ccggtgccca ccggccagct gtcgagggtc aggttgttgt ctgccatgcc cggctgggca
     5281 aagaagcggt ccgcctcgaa aggcaccggc gcgaagaagg gcatggccag ccagtaagga
     5341 aactggggat aggtgccctg cagatgcacc cggtaggtgt agcggtccac cacctcgacg
     5401 ccgggcaggt cgtagcggcg cagatcgatc caggcgccct tgtcggcgcg cccccgctcc
     5461 tcggccacca gacgcgcatt caggtccttc agcccgacga tgtagtctcc catcagctcg
     5521 agcaccgggg aatgcagacg cggatgggcc agacgcttga tctgatagac gaaatcgtcg
     5581 gcggtcagct cccgcgtgcc ggattctgga aaatcggacg ggcggcggcg ctgcgcgatg
     5641 tcgctgcgcg acagggccac atagcgggcc tgccccgcag catcgacggc aaacgccgga
     5701 tgaggctgat aacggacgcc gggacgaatc ctgatctcgt aggtgctgcg ggcgacccgt
     5761 gccggatcgg catcgggcgg cagttcgtgc cccgcccgat catactgacg caggacagga
     5821 agcgtcgcgg ccgtggccgg ttcaagctgg taggggcgct tgaggtaatg gtactggagg
     5881 ggcggttcgt agatctgata caggaaggtc gcctcgtcct cactgtagga ctggaccggg
     5941 tccagatgtt tcgggcgctg ggtgaaggcc gaatacagga tgttctgccc tcgctcggcc
     6001 actggatacg gatcgttcca gaccccgtcg cagccagcaa cggcaagacc cagcagggcc
     6061 gaaacaaaaa ggacgatcat gcgatgcatg gccgcgagtg taccggaagg tccgcactcc
     6121 gcggcagcca cgcgagggcg caagcatccg ttataatcgg cgccggattt tcatcggaga
     6181 cacctttcat gggctttctt gccggcaagc gcattctcat caccgggctg ctgtccaatc
     6241 gctcgatctc ctacgggatc gccaaggcca tgcgccgtga aggagccgag cttgccttca
     6301 cgtaccagaa cgagcgcttc aaggagcgtg tcgccaagtt ggccgccgaa ttcgacagca
     6361 cgctgatttt tccgtgcgat gtgcagaacg atgccgaaat cgccagcctc ttcgagcaac
     6421 tcggcaccca atgggacgga ctggacggac tcgtccactc catcgctttt gcgcccggtg
     6481 aagccctgga aggtgatttc ctcgacggtc tgacccgcga agcctaccgc gtcgcccatg
     6541 aagtgagcgc ctacagcttc cccgccctcg ccaaggctgc gcggccgatg atgaagggcc
     6601 gcaaggggtc gctgctgacc ctgtcttacc tgggcgccgt gcgcaccatg cccaactaca
     6661 acatcatggg cctcgccaag gccagcctgg aagcctcggt gcgctacatg gcggtatcca
     6721 tgggccctga aggcacgcgg gtcaatggca tctccgccgg cccgatcaag accctggcgg
     6781 cctccggcat cggcagcttt ggcaagctgc tggccttcaa cgagcacaac gccccgctgc
     6841 gccgcaatgt gaccatcgag gaagtgggca atgccgcggc cttcatgtgt tccgacctgg
     6901 cgagcggcat caccggtgag atcatgtacg tggatggcgg cttcaatacc accgccctcg
     6961 gcaacgccca gcccatggcc tgaacccacc gcaccgcatc aggacgaaag gcccgcactg
     7021 ctgcgggcct ttctcatttc aggcatgcct ccgtggcggc gcccggaacg aacgcgcgcc
     7081 acaatgaagc cgactcactt ctccttgttg ccttccagcg ccgccatatt gatcttcacg
     7141 tcgtaacgct gacgcagggc cgcgatgtag gccgcaaaat ccatctcggc cagcacccgc
     7201 ccgtattgct ggcgtaccac ctgcaggcgc ggatcactgg cagcggcctc cgggcgcacc
     7261 accttctcga cgcgatacag gacatagcct gcacctggca cttcgacacc cgtgtagccc
     7321 ggcagcttgt cggacgccac ccggaacact gccgcggccg cggcaggcgg aagatcctgc
     7381 gcaccgcgct gcagcacgcg cggcgcgccc cagctgagct ccacggcctc acccttctgc
     7441 agcttgccga gacgggcctc gccctccttg acggccagcc gggaggcctc atcggccgcc
     7501 agacgcttct cgatgagcgg cttcacgtcc tcgaacggac gcagactcgc cggtttgtac
     7561 tccacgacac gcgccgaaac cagcacccca ccgcccacgt cgatcgcatc cgtattgcgc
     7621 ttgttcttga cggcatcgtc cgagaacaag gcggacacca gacgttcgtt gtcgaacggc
     7681 ggtggcagct tggctgcccg gacgatccag tcgctggtct ggacattcag cttgtacttc
     7741 tcggctgccg gcttgaggct gtcggactgt tcatacaccg tgttgctgaa accttcagcc
     7801 atttcagcca gcttgccgga ggcggccttc ttgcgcagct cagccgcaat ttcgcccttc
     7861 acctcttcaa gcggacgcac cttggcggcc ttgatgcccg tcagccggat gatgtggaag
     7921 ccgaaatccg actggaccag atccgtcgta tcgccctcct tggccagggc gaacaccgcg
     7981 tcctcgaagg gtttgaccat catgccgtgg ccgaagtagc ccagatcgcc ccccttgtcc
     8041 gcagaaccgg gatcctggga attggccttg gcgagcttgg caaactcccc gggcgcagcc
     8101 ttgacctgct tgaggatccc ctcggccttc tcgcgtgccg ccttgatgac ggcctcgccg
     8161 gccgccttgt cggcctggat caggatgtgg ctggcccgcc gctcttcgcc ctgacggtaa
     8221 cggtccgcgg ccgtctcata ggccttgcgg acctcggcgt cgctgacgct cacctggctg
     8281 gcgacggcat cctggtcgag ggccacgtat tcggcgcgga cctgggccgg cacctcgaaa
     8341 cgctgcctgt tcgcctcgta gaaattcttc gccgcgtctt cgcccagttt gacggaggcc
     8401 atgaactgcg gcggcgccag aaccgcctcg ctcaccgtac gctcttcaat ctgggttccc
     8461 agaacccgtt cggcggacgc tccggacagg atgccctggg tcaacggaat aatgatctgc
     8521 tggagcgcca gatcctgacg caggcgcgat tcgaagatcg cctgattcat gccctgggct
     8581 ttcaggagcg cttcgtagcg gacctgggag aactggccgt tttcctggaa acccggcacg
     8641 gccagaatgg tatcccgcat ctgcgcgtcg ctcaccagaa gacggtgctt gcccgcatcc
     8701 agaccgagca ggcgttggtt gatcatgttt tccagcaccg cgcgacgggc ttccggactg
     8761 tccagcatcg ccggattgaa ctgatcgccc aacgaggccc gcagacggtc ctgctggctg
     8821 cgcatggcct gctggaattc agcctgagta attgcgtgcc cgcccacgga ggcgagttca
     8881 tcccccttgc ccatgttccg gacataggaa tccacgcccc agaacgcaaa gggaagcgtg
     8941 atgaggagca ggaagatctg gacgaccttc ttgttgttgc gtaccgcgtc gaacatgctg
     9001 aagaatcctc ttggctgcgg tgagcaggcg catcgagtgg ctgaaaatgc aaaaaaggcg
     9061 aacttgcgtt cgcctcaatc tgaacgaagg atccggttca tcaccctgag caagctccat
     9121 cacgtgcgca acacatgaac gggtctgagc cttggtcaag accccggacc gtcggacgaa
     9181 aagcccgtcg aatatagcaa gcgcccgtcg ggaagagcaa gcccggacgc acgtaccaac
     9241 accccgccat cggcgatccg aagcccccga aacgacaaag gctgcgggga gcagcctttt
     9301 gcgcaacggt gttcaggaaa cggccgaaaa caactcactt aggcatcatg gcggagtgga
     9361 cgggactcga acccgcgacc cccggcgtga caggccggta ttctaaccga ctgaactacc
     9421 actccatgcc gatatcgacc acctggatca gggtggtggg tgctgacggg gtcgaaccgc
     9481 cgacattcgc cttgtaaggg cgacgctcta ccaactgagc taagcacccg ctgcacacgg
     9541 actatcgccc gcactacagc ccatcagttt aatgcatcct tgagcgcttt gccaggcctg
     9601 aattttggga tcttggcgga cttgatcttg atggccgccc cggtacgcgg atttcggccg
     9661 gtccgagctg cgcgcttgcc tacatgaaac gtaccgaagc ccaccagcgt caccgagcca
     9721 ccttttttga gcgccagttt gacggctgca aaggtcgcat ccagcacacg ccccaccgca
     9781 gccttggaaa tatcggcttc cgcggaaacc tgatcgatca gttcggattt attcacttca
     9841 gtcccctttt gatgtggatc gtgcgaacga aaaaaccgaa atctggcgaa gcagggcgag
     9901 cgacgtgacg aattgcccta attggatctt gcgagtttta tagcgcgcga aaaaacgtcg
     9961 tgtcaaggca cgacggcgca cgcgcgcttg aattgcacga aaaacggaca agacccgcag
    10021 taggtattgt taatttctgc accgaacccg gccccaatac ggatttgctt cgtcaccccg
    10081 agtgaccgat cgcccccccg acacgccggg ccccgccgcg ccacgcgaca gaccgggcac
    10141 gggccggccg aagggagtga cccggcagca cgtgacttcc gccagcgccg atccaccgga
    10201 tctgacctca cggatgcatc cgcaccggga ccacaaaaca aaatggcaca agcttccgct
    10261 tgtgccattc cgaaaaccga agcttgcgcg aggatcaggc agcagccatc gccttgacgg
    10321 cagcagacag gcggctcttg tgacgagcgg ccttgttctt gtggatgatc ttcttgtctg
    10381 caacgctgtc gatggtccgc tgggacgcaa cgaaaacctg ttgcgcagca gacttgtcac
    10441 cgccgtcgat cgccttacgg actgccttga tggcggtgcg cagacgggaa cgcaggctca
    10501 tgttgtgggc gcgggccttg atcgcttgac gggcgcgctt gcgtgcttgt gccgaattgg
    10561 ccatgtttat ggtcccaaca gaatccaata aagcgttcga ttttagagga agcattctag
    10621 caatgcaagc ttttattccc gattgcattg ccgatcccga ccggcagtcg ctaccatggg
    10681 cacttttccc atcgctggcc cgccatgaat ctccttcgcg cccttgccac tgtcagcggt
    10741 atgaccctgc tttcacggat tctcggcttc atccgggatt ttgtggtcgc gcgggccttt
    10801 ggtgcgggac tcgcaaccga tgccttcttt gtcgcgttcc gcctgccgaa tctgctgcgc
    10861 cggatgttcg ccgaaggcgc gttttcgcag gccttcgtgc caatcctcgc cgaatacaaa
    10921 aaccggcgcg gacctgacga gacccgcacc ctcgtcgatc acgtcgccag cgccctcggc
    10981 gtcgtggtgc tgatcgtctc cattctgggc gctctcggcg cgcctgccat catctacgcc
    11041 accgcgccgg gtttttcatc ggacgctgaa aaatttgacc tgacggtcca gctcacccgc
    11101 atcacctttc cctacatctt cttcatggcc ctggtggccc tatccggcgg catcctcaac
    11161 acctggagcc gcttcgccat cccggccttc acgccggtgc tcctcaacct gtcgttcata
    11221 ggcatggcct tgtttgccgc gccttacttt gacccgcccg tcgtggcgct gggctgggcg
    11281 gtgttcatcg gagggctcgc ccaggtgctg ctgcagatcc gcccgctggc tcgcatcggc
    11341 atgcttccgc gcttttccct gagctttgcc gatccaggcg tacggcgcat cctgaagctg
    11401 atggcaccgg ccatcctggg ggtgtcggtc agccagatct cactgctcat caacaccgtc
    11461 ttcgcatcct ttctacccag cggcagcgtg tcatggctgt actacgctga tcgcctgatg
    11521 gagtttccgg caggcctgct gggcgtggcc ctcgggacca tcctcctgcc cagtctctcc
    11581 aagatgcacg ccgacgacaa tcgatccgaa ttttcagccc tgctcgactg gggattgcgc
    11641 ctgacactca tgctgaccct gccctcggcg ctggccctcg cgctgctggc ggttcccctc
    11701 gtcaccaccc tgttcttcca cggcgccttc aaggtcgccg acgtgtatgc cacccgcagc
    11761 gccctgatgg cctacagtgc cgggctggcc ggactgatcc tggtaaaggt ccttgcaccg
    11821 gccttctacg cccggcagga catccgcaca ccggtgcgca tcgccctcat caccctggcc
    11881 gcgacgcagc tgatgaacct cgcgttcgtg ggcgtcttcc gccacgccgg cctcgccctg
    11941 tcaatcggac tggcgtcgtg catcaatgcc ggtctgctct ttcgaggcct gaggctccgc
    12001 ggtatctatc tgccgcaacc gggctggcgt gccttcttcc tcaagctgct cgccgccctg
    12061 acagtactcg gcggcggcct gtggctgacc atgggcgccg acgaactgtg gctgacagcc
    12121 agcggcctcg accgggtgac ccggctgacg gcactggtga tcggcgggat catcatctat
    12181 ttcgccaccc tctttgcgtt gggctttcgc gtcagcgatt tccatcgccg cggcgcgacc
    12241 tgagccccgc actcaccacc cgctgtgtag aatctcgcga atcattcggg ggagaccaca
    12301 tcatgcatct gcgcagctcc agttttgccg acggcgccgt gatttccggc gtccatacgt
    12361 tctgcgttcc cgctgccgaa ggccatgtct gcctgggcgc caatcgcaac ccgcatcttg
    12421 cgtggcgcga agcccctgcc ggtacccgct ccttcgtcgt gctctgtcac gatccggacg
    12481 taccgagccg cggtgacgac gtgaatcagg aaggccgcct ggttcctgcg gatcttcccc
    12541 gcgtggattt cttccactgg gtgctcgtgg atctgcccga aacccagatg gagatcgccg
    12601 aaggcagtca ttcgagcgaa gtgactccga acggcaagcc cgggccggat cgccccgacg
    12661 gcagccgtca cggactgaac gactacaccg ggtggtttgc cggggacgcg cagatgaagg
    12721 gccagtattt cggctacgac ggtccttgcc cgccgtggaa cgattccatc cgccaccgct
    12781 atgtattcac ggtctacgcg ctggacgtgc accgcctgac cgtgactggc gccttcacgg
    12841 ggcaggacgt acgcaatgcg atgcacggcc acatcctcgc ccaggccagc ctgaccgtga
    12901 catacagcct gaaccccgcc atccccgcgt gatcatgtcg gcctgacgtt ccgcccgcac
    12961 ccttgtcacg ctgcccagat cggcaggcgc tgcgtgagca ggggaaggcg cagatgaaaa
    13021 tactccggca agccgtctcg gaatgcaggc cagtcctccc cttcgatcag cggccggcac
    13081 cagctccgga acgcagacgt cacgccgaat ccatcgggtc ggatgaactc cggcggcagg
    13141 cggcgctcca ggttcgccac cgcatcaaag ggcacgatcc gggtacgcca gcggtaaggc
    13201 acatcgccca gacgctcgat ggccaccatg ccgccttcgc cacgcagcgc cgccgccacg
    13261 gccgcctcgc caaccgcgcc ggcctgcatc gcatcggttg ccgaggccag atgccgcgcc
    13321 gcccgctgca gatagtccgc cacggcgaaa tgcaccttgc agccgagacg acggtgaata
    13381 cgctccgcaa tggcctcccc tgcacctccg agctgaacat agcctttggc agagcgttcc
    13441 tgcatggcaa tcggttcgcc ggcagcatcg tgcaagccct cagcgacgac gacagcacat
    13501 gcgccatggc gggcaacgac ctctgcgacc ttcgcgagaa aggcctcttc gtcaaaagga
    13561 acttcaggca gcaggatgac atgcggcgcg gcgccctctc catcggacgc cagcgcacag
    13621 gccgccgcaa gccagcctgc gttgcgcccc atcacctcga gcacgaaggc acgcccaccg
    13681 ccggtggtca tggccgccag atcgagcccg acctcccgta ccgacgtggc caggtacttg
    13741 gcggccgagc caaaaccagg gcagcagtcg gtcagcagga tgtcattgtc gacggtcttg
    13801 ggcacgccca ccaccgtgag gggaaagcgc cgctcctcgg ccgcagcggc gatctgccgg
    13861 caggtttcca ttgagccgtt gccgccgttg tagagcaagg ctctcacgtc gtgcgcggcc
    13921 agcaccgcaa acaaccgatc atattgggcc ggattgcgtt cgaccggatc cagatccagc
    13981 cggcaggatc cgaatgcgcc gcctggcgtc cgtccaagac aggccagatc ggcgtccgtc
    14041 aaggcgctcg tatccaccag acgctcctgc agcacaccca tgatcccatg ccgggcggcc
    14101 agcacccggg cgatttgctg cggcacgcgc cgcgccgccg tgatgacccc ggccgcggag
    14161 gcgttgatca ccgcagtcac gccgccggac tgggcataca ggagatttcc gttcacaaca
    14221 cactggctcc caggttacgg gacaggacca cgggatgagg gtcttgcctc agcaaaagac
    14281 cacgatcgcg agaaacaatt ccaacgcgga tgctcgcctc gtccatcatg caggtcacgt
    14341 ccccgcccgc gacacccgca gaccgtgcgg attcaccgtg atcgcagccg atcacctgct
    14401 ttgcattcag cacgcagaca cgccaccacg cgggccagca cccaagaaaa agccggccct
    14461 caggccggcc ttcttgacag gacaagcgac gcttacttca gcgcgccaaa cgcccggtca
    14521 cggatctctt caaccttgcc cagaccaccg atcttgcgat actggggggc cgcggcgtca
    14581 ccagaagccg cccactcgga gtagtaggcc accagcggct tggtctggga gtgatacaca
    14641 tccagacgct tgcggacggt ttcttccttg tcgtcgtccc gctggatcag cgcttcgccg
    14701 gtttcgtcgt ccttgccgtc caccttgggc ggattgaaca tgacgtgata ggtccggccg
    14761 gatgccacgt gcacacggcg accggacatg cggccgatga tttcctcgtc cggcacatcg
    14821 atctcgagca cgaagtcgat gggcacgccg gcggccttca tagcttcggc ctggggaatg
    14881 gtgcggggga aaccgtcgaa catgtagccg gcctggcagt cggcttcctt caggcggtcc
    14941 ttgaccaggc cgatgatgat gtcgtcggac acgagcccgc ccgcatccat gaccttcttg
    15001 gcctcgatgc ccagcggggt gcccgccttg accgccgcac gcagcatgtc gccggtggag
    15061 atctggggaa tcccgaattt ttccttgatg tagttggcct gggtgccttt gccggcgccc
    15121 ggcggtccca acagaatcag acgcatgcct taccctcctc tggttattgt cgctgcgaca
    15181 tggggtggtg aaactaacgc gaaactgacg ctacgtcaaa cacgcggcgc actcgctcga
    15241 gatcttcggc cgtatccaca ccgcccggcg gtgcgtgatc gagcaccatc acgcgaatgg
    15301 ggaagccgtg ggccatcgca cgcagctgct ccagtgcttc ccactgctcc agcggcgatg
    15361 aggcgagggc cgcgtagcgc ttcaggaaac cagcccggta ggcgtacagg cccacatgac
    15421 gataggccgg aaaccccgcc ggcaacgcgt cccgctgctg ggcgaaggca tcccgtgccc
    15481 agggaatcgg tgcccgcgaa aaatacaggg cgcgaccgcg agcatccagg accagcttga
    15541 cgacgttcgg gttgaagaat tcctccgcgg acgcgagcgg atggcaggcc gtggcgatcg
    15601 ccgcttcggc atcggcggac agcgtggcgg ccgcatcggc gatgagtgag ggtggaatca
    15661 gcggctcatc accctgcaca ttgacgacca ccgtatcgtc gtcccagccg agccgagtgg
    15721 cgacctcggc gagacgatcg gtaccggacg gaagttcgcg gccggtcagg atgacctgcc
    15781 cacccgccgc ctcgacgcgc tcggcaatgt ccgaatgatc cgtcgccacc cacacctcgt
    15841 cggcgccagc cagacgggcc tgatcgagca cgcgcaggat catgggccgg ccggcgatat
    15901 ccagcaacgg cttgcccggc aaacgacttg aggcgtagcg ggccgggatg acgatgcgaa
    15961 agcccatgcc tcagcgcacc tgcgcgactt cgtcgtcggt cagggggcgg gcctcctctt
    16021 ccagcatcac cggaatgcca tcgcgaatcg ggtaggccag gcgagcgctc acgctccaca
    16081 gctcctgctt gtccttgcgg tagtcgagcg gccccttggt gatcgggcac accaggattt
    16141 caagcagacg gggatccatc gatcttctcc agaatcagtt cggccgcacg ggacggcaca
    16201 tgcgcccgga cgggccacac ccagctttca tgcggggcga aaggcgcgca ttttaccgca
    16261 tccttctcgg tcatcaccaa ggcatcgacg ccctccaggg ccacatcggc gggcgtgaac
    16321 acgtgatgat cgggcagcgg acaacgctcc accgcaagcc ccatcgcctc cagctgggcg
    16381 aagaagcgct ccggacggcc gatcccggcc agcgcccgca gacgccgccc catcagcgcg
    16441 gacggcggac ggcgctctcc gcggtcggac agacgctcga aggctgcgcc cgccagatgg
    16501 aattcgaaca ccggcgtggt cccacaggcg cgacgcacgg acggcgccag cgcaccatgg
    16561 gccagcacca gatcagcccg ggccaggcgc gtcacacctt cacgcagcgg accggctggc
    16621 aggcgcaggc cgttgcccag ggtggcctga tcgacaacca cgacctccac atcccgctcg
    16681 aggcggtaat gctgcagacc gtcgtcacag acaagcacat ccacctccgg gtgcgcggcg
    16741 agcaaggccc tgcccgccgc cgggcgatcc cgccccaccc ataccggaac acccgcaagg
    16801 tgcgccatca gcacgggctc atcgccaaag tgcgcgggat cgccgtccgg cggcacttcg
    16861 gccacaccct cgacgtgccc gccatacccg cggctgacga tgcccggccg acgacccgca
    16921 gccttcaggt gctcgaccag ccagagcgtc accggggtct tgccactccc cccgaccgca
    16981 atgttgccca ccacgatcac cggtgcggga aggcgctcgg tacgcagcca gccgttacgg
    17041 aacagcccgc ggcgaagacc ggcgacggca ccgaacagca gcgacagcgg catcagggcc
    17101 caggcgacgg cgccccgccg ggaccagaag gcgggggcac gacggggcat ggaggcgtcg
    17161 gatcaggtca ggcccgcgcg ccgctcagcg ctgggcggcc tgggtcgcga aggagatgtg
    17221 aacgaggccg gcctgctggg ccgcctgcat gacgtcgatg acgcgctggt gggtcgcctt
    17281 ggcatcggca ttgatgatca cgaccggatc cttggcgccc tggggcacca gcgcctgcag
    17341 cgccgcggcg atggcctgga cggatgtccc ggacaccgcc cgcttgttga ccaccacgtc
    17401 gccggtggcg gtgaccgcca cgttgagttc caccgttttt tcggcactgg cctgagcgtc
    17461 ggcggtgggc agattgatct ccaggccggc aaaacgcgaa tacgtggtgg tcagcatcag
    17521 gaagatgatg atcaccagca gcacgtcgat catcgggatc aggttgatct ccggctcctc
    17581 gcgggactgg cctcggcgga agttcatgac aggacctcag cgacgctcgc cgtgcaccac
    17641 ttcgaccagg cgaatcgcct gctgctccat ctcgatcacc aggctgtcca ccgtggcgcg
    17701 gaagtgacgc cagaagatca tgctcgggat ggcaatgacc aggccgaagc cggtgttgta
    17761 gagggcgatg gagatcccgt gggccagctg ctgcgggttg gacccgctgg gcgcctgaga
    17821 gccgaagatc tcgatcatcc ccactacggt gccgaagagg cccatcagcg gactgatcga
    17881 ggcaatcgta cccagggtgg tcaggaagcg ttccaggtcg tgggtgaccg cccggccggc
    17941 ttcctcaatg gactctttca tcacctcgcg ggggctccgc acgttacgca gcccggccgc
    18001 gaagacgcgc cccagaggcg aactcagggc gacccggctg atcatctcgg gcgtggcgcc
    18061 cgtctggcgc agttcggcca caaccttgtc gagcagtccg tcggggatga tcttgctgcg
    18121 gcgcagcgtg atgagacgct cgatgatcag ggcgacggcg acgatggaag caaacagcaa
    18181 gggccagatg ggccagccgg cagcctggag aatggcgaac acgcggggga ctcctgcgat
    18241 gcggaacaag cttcggaatt taacctgccc ttctgacaag agcaagaatc ccgcgcccgc
    18301 cgacaatcca caaaatctgt ggataaatgt gtggatagaa accgtgtgaa ggcgctaacc
    18361 ctccagtcca taaggacttt tcctgctcgg atcaaaaatg gggcgacgaa cacaatccat
    18421 acttttcaat aacttacgga aaacatgcga agtcaagccc ggcggccaga atattttttt
    18481 atggcgtgct ccaggtgtgg attccgctag aatccgcgcc catgaatccc cccgaaaatc
    18541 accctgccac acccgacagc cgcgtgattt ccgtcgccga cctgaaccgg cgcgcccgcg
    18601 aggtgcttga ggggacgttc cccttgcaat ggatcgccgg tgaggtgtcg tcactgaccc
    18661 gcgctgcctc cggtcacgtc tacttcaccc tcaaggacga cgaagcccag gtgcggtgca
    18721 cgctgtggcg caatcgtgcc cagctcctgc cattccggct cgaacacggc atgcaggtgg
    18781 aaatccgcgc ggtggtcacg ctgtatgaag cccgcgggga cttccagctc aacgtggaca
    18841 ccgtacgccg cgccggccag ggcaatctct acgaagcctt cctgcgtctc aaggcgcgtc
    18901 tggaagccga aggactgttc gcctccgaac gcaagcgtcc gctgccgacc tacccgcggg
    18961 gcatcgccgt cgtcacctcg ccccaggcgg cggcctggca ggacgtgctg gcggccctcg
    19021 cccgccgcgc gcctcacgta ccgatctgcc tctacccgac ggccgtccag ggcagcgacg
    19081 ccggtgcgcg catcgctgcg gccctgcggc tggccggcac gcgggctgtg caggacagca
    19141 ccgacgtcct cctcctcgtg cgcggcggcg gcagtctgga agacctggca gccttcaacg
    19201 acgaggccgt cgcccgcgcc atccgtgact gtccgctgcc ggtggtgtgc ggcgtaggcc
    19261 acgagaccga cgtcaccatc gcggactttg ccgccgacct gcgcgctgcc acgccaaccg
    19321 ccgcggccga actggccagc gccggatttc acgccctgca cgaacgcctg agcctgctgg
    19381 ccggtcgcct gaaccgtgcc atggaacgcc gcctgactca cgctgcccaa cggctcgacc
    19441 ggacggccct gcgtctgcgc cacccccgtg aacgcctgcg cgaagcggca ctgcgcctcg
    19501 accacctggc ccgtcgcctg cagaccgccg gacacgagcc cccggcccat gggcgcaatc
    19561 gactcgatgc cctgcgcctg cgtctgtccg cacgtcgccc cgatgcaagc cgggcacgcg
    19621 accgcctcga gagtctcgcc ggaagacttc agcgggcgct ccgtatccag cttgaacgcc
    19681 gccgcgcccg cgtcgacgcc ctcaccgaac accttaccca cctcgacccg cgcagcgtgc
    19741 tcgcccgtgg ctacaccatc acccgcgacg cccgcggcag ggtcctgcgc aacgcggccg
    19801 atgcggtgcc cggtcaggac attcgggtcg agttccacga cggcgaggcc gacgcccgtg
    19861 tcatggcccg gcgcctgcgc tgacaccgat ttgtcgcaca gcagcaattg tcagtccgcc
    19921 tcaaacccga ctagaatggt cgggcaattc tgcccacccc tacaagaatc accggagaat
    19981 gacccatggc tcacaccctg cccgaactgc cctacgccaa gaacgctctt gccccggcca
    20041 tttctgaaga aaccctggag ttccactacg gcaagcatca tcaggcctac gtcaccaacc
    20101 tgaacaacct gattcccggc accgagtacg agtccctgtc cctcgaagag atcgtcaaga
    20161 aggcccccgc cggcggcatc tacaacaact ccgcgcaggt ctggaaccac accttcttct
    20221 ggaattccat gaagccgaat ggcggcggcg ctcccagcgg cgccctggcc gacgccatca
    20281 acgccaagtg gggctccttc gacgacttca agaaggcctt ccaggcgtct gccgtcggca
    20341 acttcggttc cggctggacc tggctggtca agaaggctga cggctccgtc gacatcgtca
    20401 acatgggcgc cgccggcacc ccgctgacca ccggcgacac cgccctgctg tgcatcgacg
    20461 tgtgggaaca cgcctactac atcgactacc gcaacctgcg tcccaagttc gtcgagacct
    20521 tcctcgccag cctcgccaac tgggatttcg cagcggccaa cttcgccgcc tgaccggcct
    20581 ccggtcaacg caaacggccg ggcatctgcc cggccgttgt gtttgggagt ccggagtcac
    20641 gcacctggca cgcgcatacc tcagtgatag accgccggct gggtcatcaa ggtctcgtag
    20701 ccgtcgatcc agtcttcgta gtcgtccatc tcgtgggggg tcgcctcgag atcgaggagt
    20761 tcctggcgaa agcgacgtgc cgcctcgtcc cggaacatca ccccgacacc tcgtcgcttg
    20821 tcgataacct ccaccgcatc gtgggcggga taatcgacca catataacac cggattgttg
    20881 agtaccacgt tcatgaccgg actccttcgc attgtttcga tcttgtggat ctgacctgct
    20941 ccttacatgg gaacgtttcc agtcgattca agttcccggg cgcgttgtat ttccatgcgg
    21001 tgcagcctaa gattcagcca agccagaatc ctgcccgccc ctccaccttc attgaccccg
    21061 gcatgaacga ctgcgacgaa cccgacgacg aagtcaccag cctctctgcc cggctggccg
    21121 ccctccggac cgaacaccgg gacctggacg ccgtcatcgg ccagctgatc agcgccccca
    21181 tcaatgacga actgctcctg cgccgcctca agaaacgcaa actcctgctc aaggatcgca
    21241 tcgccatctt cgagcgcatc ctcgatccgg acgttccggc ctgaaccgcg cgcttagcct
    21301 cgtcccttgc cccgacccgt acgccacgca ccgtcccacg catgaccctc catcgacctg
    21361 ccggggccga cacccttgcc ctgcatgccg gccagacccc ggacgcgccc catggcgccc
    21421 gcgcggtgcc gatccatttc tcgaccagct ttgtcttcag cgacgtcgac caggccgcag
    21481 ccctgttcaa cctggagcag cctggtcatg tttactcgcg tctctccaat cccaccaatg
    21541 cggtgctgga ggagcgcatc gccgcactgg acggcggcgt gggcgccctt gcgtgcgcca
    21601 gcggtcaggc cgcactgcac ctcgccatca cgaccctcat gggcgcgggc ggacacatcg
    21661 tcgcctcgcg agcgctgtat ggcggttccc acaacctgct ggcctacacg ctgccgcgct
    21721 tcggcatcgc caccaccttc gtcgatcccc gcgacccgga tgcctggcgc gccgccgtac
    21781 gcccggaaac ccgcctcctc ttcggcgaga gcctcggcaa ccccggcctg gacgtcctcg
    21841 acatccccac cgtcgcgcag atcgcccacg acgcccgcgt gcccctgttg gtggatgcca
    21901 ccctcgtcac gccggccctg caatgcccga tcgaccaggg agccgatctg gtcctccatt
    21961 ccgccaccaa gtttctcggc ggacacggtg tcgccatcgg cggtctgctg gtggacggcg
    22021 gctgcttcga ctgggagtcc gccggccgtt tccccaccct gaccgagccc tacgaaggct
    22081 ttcacggcat ggacttcgcc gaggagtctc ccatcacggc cttcctgctg cgcgcccgcc
    22141 gcgagggcct gcgggacttc ggcgcctgcc tgtcgccaat gaatgccttc cagatcctgc
    22201 agggcatcga gaccctcggt ctgcgcatgg agcgtcacgt cgccaacgcc cgggagatcg
    22261 tccagcgcct gctgggccat ccccaggtcg aaaacatcgc ctggccggaa ctggacggcc
    22321 accccgacca ggctctggcc cgccggctgc tgccgaaagg ctgtggcggc gtcgtgagct
    22381 tccgtctaaa gggcgggcgc ccggccggcc gcgccttcat cggcgccctg cgcctgttct
    22441 ctcacctggc caacgtgggt gacgccaaat ccctcgccct ccaccccgcc agcaccaccc
    22501 acttccgcat gacaccggag gcccttgccg ctgcgggcat cggcgaaggg acgatccgcc
    22561 tctcggtcgg actggaatcc gtttccgacc ttctcgacga cgtggatcgg ggcctgcacg
    22621 cggcccagaa agcgaccgga tgatgtccac cttgaaattt tgtcaatcac ggacagtggc
    22681 cactgtccag ccgcgccgaa cggcgggccc tgtcactgcc gccgaaaacc cacgcgctca
    22741 tcgccagggg gcatccgccg cccctgaaac tccccgacgg gcggcctgat ccatggatct
    22801 ccgcgtcacc ctgtcgccaa acgcccccag tgagtgcccg gtcttcgcct acacgggtgg
    22861 gcgcagcccg gcaccggacc ggccgtggat cgtcttcctt cacggtgccg gcaacgacca
    22921 cagcgtctgg atgctccaga cccgcgcctt cgccgcacgc ggctggaacg tgctcgcgcc
    22981 cgacctgccc ggccatggcc gcagcggtgg gcaagccctc gacaccatcg aggccctggc
    23041 ggactggacg ctgcgcctcc tcgacgccgc cgccatcgag cgttgcgccc tggtcggaca
    23101 cagcatgggc tcattaatcg ccctgcacct cgcctccatc gcgcccgaac gcgtcttccg
    23161 cctcgccctg gtgggcagcg tcttcccgat gcccgtggca ccggccctcc tcgacaaggc
    23221 tcgaatcgac ccgcccgccg cccacgccct gatcaatcag tggtcctttg cgcccgcaac
    23281 cctgctgggg cgcagcgccg taccgggcat ccaccctgcc gccgtgaatg aacgcctgct
    23341 ccaacgccag ccggtcggca ccctgcctgt cgcgctgcag gcctgcaatg cctacgccgg
    23401 cggattcgaa gccgccgcac ggatccactg cccgaccctg ctcctcagcg cggcccagga
    23461 tcgcatgacg ccgcccaagg ccctcgcccc cctcgccgac gccttcacat ccagcgccat
    23521 gccgcagcgc gtcgtgattc caggtgccgg tcatgccatg atgaacgaag cgcccactgc
    23581 ggtgctcgaa gccctggagc gcttcatgac gccccctgcg ggctgaatct cacgttccac
    23641 actgcagcag ccagttccac aaaaccataa cggagacaac ccttggcgca ctcaaacctc
    23701 cccacggacg acgaagcccg cgcagcggag ctgccctttc ccaccctgcg cgaggtcggc
    23761 ttcgcggccc cgttcgcctg ggtcggtcgg ggcctcgcgg atctcaaggc caatcccaca
    23821 gccagcctgt tctatggctt ctgctttgcc ggcatgggcc tgctggtcac cttcatcttc
    23881 gagcacgcct accaatacac ctcggccgcc accacgggct ttctgctgat ggctccattt
    23941 tttgcgatgg gcctgtatga actgtcccgg cggcgcgaac tcggccagcc cgtgcaactc
    24001 ggcccgaccc tcaccgtctg gcggcgtaac gcaggcaata tcgcagtctt tgccgccgtc
    24061 ctgaccgtca tcttcctgat ctgggcacgg gcctcgctgg tcgtcttcgc catcttctac
    24121 acccgcgaac tgcccaatct gagcggcttc ctcacccagg tgctgtcctt cgagaacatc
    24181 gagttcctgg ccgtgtattt tgccgtcggc atgatcttcg ccctgctggt gttcgccgtc
    24241 agcgtcgtgt ccattcccct catgctcgac cgcaaccagg atgcggtcac ctcgatgatt
    24301 gccagcatcc gcgccctcgc cctcaatccc ggcccactgc tggtgtgggc cgtactgatc
    24361 gtggccctca cgatcctcgg cttcctgagc ttccatctcg ggctcgtcgt caccatgccc
    24421 atcgttggtc acgccacctg gcatgcctat cgggatctcg tcgcccccct tccgcgtcac
    24481 ggatgagtac cggccggcac cggcggaaca tccgcttact gcaccgtcac gcggcctgtg
    24541 gcacaaaagc gtttccgccc acctttccgg agtgtgcccc atgggcctga agcacctgac
    24601 cgcctgtctt gccatcctga cgatgaatct cacggccgcg cgcgccgccg acgcgccccc
    24661 gaccgtcacg gccccgctgg ccgacctgag cgccgaagcc agccgcagcg cccccaatga
    24721 ccagctccgc gcccttgtct atgccgaagc cagcgatgcc aacgccgccg acgtcgcgcg
    24781 gcgggtgaac ggcctgatcg cccaggcact ggccaccgcc cgcggatacc ccgcagtcaa
    24841 ggtcaaaagc gccggcaaca gcaccacgcc ggtttacggc aagagcgggc gggccatcga
    24901 cgcctggcgc atgcgctcca gcctgagtct ggaaagcggg gacactgccg ccttgtccga
    24961 actcctcggc aaactccagc aaagtcttgc ggttgcgagt ctcaacgctg cgccctctcc
    25021 ggaaacctgg cgcaaggtgg aagacgaggt catcggcgac gcgctctccg ccttccagaa
    25081 ccgcgcccgg ctggtggccg gtggacttgg acgcggctgg cgcatccggc agatctcggt
    25141 caataccagc ggcggacgcc cccagccggt catgccctac gcccgcgcgg cgatggcgat
    25201 tgctgaggcc gctccggcgc cggtggaggc aggcgacacg ccgatcaccg tcacggtcac
    25261 cggccagatc gagctgctcg accgctgaac cccgcaacag ggcttgaatg ctgcgcggcg
    25321 caggctaaac tgcgcgtcat gattgcagtg attcaacggg tgctggaggc gcgcgtgacg
    25381 gtggacggac gcatcgtcgg cgcaatcggt ccgggcctcc tcgccctggt ggccgtcgag
    25441 aagggtgacg acagcccacg cacggcgcgc atggcggagc ggctcatgtc ctaccgggta
    25501 tttgccgatg aagcaggtcg catgaaccgg tccctcgccg aaaccgccgg cggtctgctg
    25561 ctggtctcgc aattcacgct ggccgccgac accgcccgcg gtacgcgccc cagcttcacg
    25621 ccggccgctg caccggaaga cgcgcggcgc ctgttcgatg aactggtccg tcatgcccgc
    25681 gtccgtcatc ccgatgttgc caccggcgaa ttcgccgccg acatgaaagt cgccctgatc
    25741 aacgatggcc ccgtcacttt tcgactcacg acctgaactg cgcacggcgc ttgctgccgc
    25801 ctgtgtggcg gccgtgctgg caggctgcgg cagtgcgccg ccccgcacga cctcccagcc
    25861 gcccccatcg gtcatcgcac cgccggaacg ggcgtggttt tcactgcccc atcccgacca
    25921 cgcccaggag gtcgtgctgt acgccctcgg gctgctcgac acaggctacc gtttcggcgg
    25981 tcgcaatccg gaggccgggc tcgattgcag cgggatggtc agcttcattg tggaacaggt
    26041 gagcggacag cgcctgcccc acaacgccgc gcagatcgcc gagcggaccc gcccggtcgc
    26101 ttccggcgcc ctgcagcccg gtgatctggt gttcttcaac accctcggac gcgcctattc
    26161 ccacatgggc atctatctgg gtgacgggcg cttcatccac gcccccaaca gtcgcggcaa
    26221 ggtgcgggtc gaccgcctcg acaaccg
//
LOCUS       DAOQPA010000037        25930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_878_length_25930_cov_19.800889, whole genome shotgun sequence.
ACCESSION   DAOQPA010000037 DAOQPA010000000
VERSION     DAOQPA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 25930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25930
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_25930_cov_19.800889"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PKX01_07275"
     CDS             <1..165
                     /locus_tag="PKX01_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HMZ75963.1"
                     /translation="QYFAYIEITPGEPQNLWREYEKLTGAKSNFAMQHIEGLPDIYPV
                     FRELFKKKLA"
     gene            162..1691
                     /locus_tag="PKX01_07280"
     CDS             162..1691
                     /locus_tag="PKX01_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HMZ75964.1"
                     /translation="MSARRPKRKKALPAPGEWTFDLIETYHAEIDRVARDFGLDTYPI
                     QLEIITSEQMMDAYSSVGMPVNYHHWSFGKSFLQTEKSYRRGQMGLAYEIVINSNPCI
                     AYLMEENTMTMQALVIAHAAYGHNSFFKGNYLFRQWTNADAIIDYLLFARNFLGECEQ
                     RHGEEAVELLLDSCHALMNVGVDRYKRPAKLSLEKERTRQRERAEYLQSQVNDLWRTL
                     PIQQIKAQAVEERRFPPEPQENLLYFIEKNAPLLEPWQREVVRIVRKVGQYFFPQRQT
                     QVMNEGWATYWHYTLLNTLYDEGLLSDSFMLEFLHSHTNVVYQPRYSEPWYRGLNPYA
                     LGFAMWQDIRRICEHPTDEDRAWFPDMAGSDWRETFDFAMRNFKDESFIAQFLSPKVT
                     RDFRLFAILDNDKDPKLKVSAIHDESGYRRVREILSDQYNLGSREPNIQVWNVDLRGD
                     RSLTLRHQAYMRRPMGGDAEEVVKHIGRLWGFPVRLEGVQDDGTVQKLHEWSPKHAQD
                     A"
     gene            1774..2766
                     /locus_tag="PKX01_07285"
     CDS             1774..2766
                     /locus_tag="PKX01_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HMZ75965.1"
                     /translation="MSADDFHRILGVKPGASAAEIKRAYRRQAMRWHPDRNADPDAHD
                     QFKRIREAFERLSVGASQVDNDGDADTPPPAAPRGEDRRVEVTLDLTEAALGTTVDVT
                     VEGRTSCTACGGSGQRSYGRTTMCGACHGSGRVRSKNGLDNCTQCSGKGYFTDRRCPD
                     CDGRGWHPSERRIEVTVPPALLPGDELRVPGQGGPAPEAGTPGDLYITVRARPHPLFR
                     LDGRNVHLTVPVSVFRLLAGGEILVPTLSGLETVQLPEGAGQPELRVTGHGFPARGKR
                     PAGDLHLHLKTELPAYLSQEQKAMLEMAEQVLLHHLDTQSPALALWKQVLEQHR"
     gene            2820..4088
                     /locus_tag="PKX01_07290"
     CDS             2820..4088
                     /locus_tag="PKX01_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HMZ75966.1"
                     /translation="MPPKTPELLAPAGSLAMLDTALAFGATALYAGQPRYSLRVRNND
                     FGDLPILAEGIRRTHAIGARFYLVANIYPHGAKVKTFLKDIAPVIALAPDALIMSDPG
                     LILLVREHFPHMPIHLSVQANTVNAAAVRFWRQAGISRVILSRELSLDEIASIRQDCP
                     DTELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNACRWEFKTHGARTCSAGDVH
                     LLEEPQKRAGEYMPIEEDEHGTYILNSRDLRAIEHVERLVEMGIDSFKIEGRTKSPYY
                     VARTVQAYRQAIDDAVAGRRLDPALLGQLEGLANRGYTDGFYRRHTPHATQNYLRGHS
                     ESGRSLYVGDVVAFDAARGLAEIASRNAFSVGDRIELIQPAGTREIRIDRLEDADGAP
                     LERVPGSDHRAWIPLPPDCVGAFVARFIHP"
     gene            4181..4399
                     /gene="rpmE"
                     /locus_tag="PKX01_07295"
     CDS             4181..4399
                     /gene="rpmE"
                     /locus_tag="PKX01_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HMZ75967.1"
                     /translation="MKADTHPKYAEVEVTCSCGNVFTTRSTLGKPQLHVEVCAACHPF
                     YTGKQKIVDTAGRVERFRQKYGNVQRLG"
     gene            4493..6091
                     /locus_tag="PKX01_07300"
     CDS             4493..6091
                     /locus_tag="PKX01_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75968.1"
                     /translation="MPTPLTGWPLAVLIGVYLLVGMGHVPWRGDDLTYLGPIHAILTD
                     GSWLIPSIAGEPFYEYPPLYYWLGAVLARAFGWLMPVHDAARLASSLCAAATLYWTGL
                     AARRLYGDNTGAPAILLGLGSLGLVVHAHETQPLLAQLAGMALCYAGLADMVRRPWAG
                     ARQAGIGAGLTFLAGGLPGMFVTLPLFFAMPLLCDNCRNGAVPRALAGGLVVALLLGG
                     SWPALLGWLHPEALASWWNDELAASLPHGNHLQALGKMIPLLGWFAWPLWPIAGWALW
                     KNRRALNTPPVALPIIASLLAMVAVVTTGPLRPASTLPILPPLCLLAALGVGTLRRGA
                     ANALDWFAIMAFAFFAILVWIGWSALYFGWPPGLGRQLARLAPDLVRSPSTIAAVTGM
                     FLSLALFALPVLTTRSTLRGATNWAIGMTLLWCLAVALWQPWFEHTKNYAPVAQALAR
                     ELELHPAPCITRKGLNDTQRAALDYFNGIKTIHHSTDQDCPAFLVYSADGLPYRPPGD
                     WRTSWEKRLGGGRKTELFRLYRHD"
     gene            6084..7718
                     /gene="pgi"
                     /locus_tag="PKX01_07305"
     CDS             6084..7718
                     /gene="pgi"
                     /locus_tag="PKX01_07305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ75969.1"
                     /translation="MTDLTPFDLDASDAGTSLSRLASAPELTHLRALFSKDPARFQAF
                     SCRYGEWLLDYSRQRMDAAIRSQLLALAEESRLRQGIDAMLSGAIVNVTEQRAVLHCA
                     ARLSPHTPLNARNQIEETLARVMALVDRIHAGSLTGSRGMPIRHVVHLGIGGSDLGPR
                     VLSEAFSTAYPRRLKVSFAANVDASEIRSILIDATPEETLFVVASKSFTTAETLSNAR
                     IARQWLTDQLGPDADVRAHFAAISNNVHAASSFGVPEERIFPLPEWVGGRFSVWSAIG
                     IPIMLEFGTPVFLEFLAGGRDMDAHFATAPFDSNLPVLAALIGLWNASYLRINCHAVL
                     PYCHALRSLPAYLQQLEMESNGKHVRRDGSALSYPTAPILFGGAGTVGQHAYHQLFYQ
                     GTETVALDFIVPIPDTPTPATRSLVGNALAQAAALMHGRTEDEARAELTQRGLAQDEV
                     ERLAPHLVCPGNQPSSTLLMPALTPFTVGQLIALYEHKVFAQGWMWGLNSFDQYGVEL
                     GKQLARRLESGDMSDADSSTQGLMADIARRWEPAKE"
     gene            complement(7784..8065)
                     /gene="ftsB"
                     /locus_tag="PKX01_07310"
     CDS             complement(7784..8065)
                     /gene="ftsB"
                     /locus_tag="PKX01_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HMZ75970.1"
                     /translation="MRWPLLILVLLVAALQYPLWIGKGGWLRVWEVEQQLAEQKEGNR
                     KLEQRNTGLEAEVRDLKSGYEAVEERARFELGLIKPDEVYVQVPQKTPQ"
     gene            complement(8113..9402)
                     /gene="eno"
                     /locus_tag="PKX01_07315"
     CDS             complement(8113..9402)
                     /gene="eno"
                     /locus_tag="PKX01_07315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMZ75971.1"
                     /translation="MSAIVDVIAREVLDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDAARYLGKGVLQAVENVNTEISEALVGLDAEEQAFLDRTLIELDGTEN
                     KSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGGMSMPVPMMNVINGGAHANNSL
                     DIQECMIMPVGAKSFREALRCGAEIFHHLKKLTDKKGYPTTVGDEGGFAPNVSGTDEA
                     LNMILEATANAGYEPGRDVVLALDCAASEFFKDGKYELHGEGLSLSSEGFTDYLATLA
                     DKFPIISIEDGMAEGDWAGWKILTERLGKKVQIVGDDLFVTNTRILKEGIEKGIANSI
                     LIKINQIGTLTETFAAVEMAKRASYTAVISHRSGETEDSTIADIAVGLNAMQIKTGSL
                     SRSDRISKYNQLLRIEEDLGDTAFYPGLSAFYNLRTR"
     gene            complement(9497..10336)
                     /gene="kdsA"
                     /locus_tag="PKX01_07320"
     CDS             complement(9497..10336)
                     /gene="kdsA"
                     /locus_tag="PKX01_07320"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMZ75972.1"
                     /translation="MKLCGFDVGLDKPFFLIAGPCVIESREMALDTASALKEICAELA
                     IPFIYKSSYDKANRSSGKSFRGMGMEKGLEILADVRATVGVPVLTDVHTEDEVPAVAA
                     VVDVLQTPAFLCRQTDFIHAVAASGKPVNIKKGQFLAPGDMKNVVDKAREANGGADTI
                     MVCERGASFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTSSGGQREFVPV
                     LARAAVAVGVAGLFMETHPDPAKALSDGPNAWPLPRLKALLKTLKDIDALVKGQGFIE
                     TQA"
     gene            complement(10348..11991)
                     /locus_tag="PKX01_07325"
     CDS             complement(10348..11991)
                     /locus_tag="PKX01_07325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMZ75973.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMGKRNNFTTGQIYEAVIKKE
                     RRGEYLGKTVQVIPHITDEIKLYIKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     IEEGRQGTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPAD
                     ERRKIALFCNVMPEAVIECLDADSIYSIPGMLHDQMLDEIVCHKLGILARAADLSVWE
                     RLIDALNNPQHHLDIAFVGKYVDLTESYKSLIEALNHAGMHTRSKVSIHYIDSEDIEK
                     SGCDVLKRMDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDV
                     AGMNGAHSTEFDKHTEFPVIGLITEWLDASGKLEKRGEDSDLGGTMRLGGQVCRLTEG
                     SLAREVYGRPEITERHRHRYEVNNSLLSRLEAQGLRVAGRAPGTDLCEMIELPTEGPN
                     AHPWFVGCQFHPEFTSNPRQGHPLFTAFVKAALAHQHAG"
     gene            complement(12097..12639)
                     /locus_tag="PKX01_07330"
     CDS             complement(12097..12639)
                     /locus_tag="PKX01_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HMZ75974.1"
                     /translation="MLMDRRCASLLVVDVQERLLPAIDDGAAVLDHCVWLVRVARQLG
                     VPVVLSEQYPQGLGPTATALQEAAGDAAVVYKTAFSCVADGSLSGTPVDARRQVVVTG
                     TEAHVCVLQTALGLRWQGKEVFVVAEAVGSRKPADKELALARMRAHGIEVVSREMVAF
                     EWMERCTAPEFRTVSRNFIR"
     gene            complement(12650..14626)
                     /locus_tag="PKX01_07335"
     CDS             complement(12650..14626)
                     /locus_tag="PKX01_07335"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HMZ75975.1"
                     /translation="MSYPIAAAPLWTPSPERVAAALITGFAQRASARWQRPLDGYAAL
                     HAWSVAHPEEFWTSVWEDGDVCGVRGDTVLADGERMPGARWFPDARLNYAENLLRSRD
                     DADALVFWGEDKVKNRLSHGDLYRQVAHFAAALQEQGVGAGDRVAAWMPNLPETIVIM
                     LAAASIGAVFSSASPDFGVQGVLDRFGQIEPKVLVAVDGYYYNGKVVDCLDRLAAIAD
                     GLPSLRRVVVVPYVHASHDLSHIHHARLLPEFVRPFLAETEIPFVRLPFAHPLFILYS
                     SGTTGVPKCIVHSAGGTLLQHIKELRLHGDVRPGDRLFYFTTCGWMMWNWLVSGLASG
                     ATLLLYDGSPMVGQGSILFDFAEAERMTHFGTSAKFIDGIAKLGVAPRETHDLTSLRA
                     MFSTGSPLVPEGFEYVYRAVKADLCLSSISGGTDIISCFVLGNPTLPVYPGEIQCRGL
                     GMAVDVFDETGTPIQGEKGELVCTAPFPSMPVGFWNDPDGSRYRAAYFERFEHVWCHG
                     DYCEITAHAGLIIYGRSDATLNPGGVRIGTAEIYRQVEKCDAVLESLVIGQQWPAGSG
                     DVRVVLFVKLREGRVLDDALCEQIRQTIRDNATPRHVPARIVQVDDIPRTRSGKIVEL
                     AVRAVVHGEPVKNLEALANPAALDNFRSRPELVN"
     gene            complement(14675..15097)
                     /locus_tag="PKX01_07340"
     CDS             complement(14675..15097)
                     /locus_tag="PKX01_07340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ75976.1"
                     /translation="MNTPASAPDRKLLLTTRIPVRWGDMDFYGHVNNTVYFRYYEQAR
                     VEWLEAFGFAVGPTAETAPVIINASCTFLIPVNYPATVIVRLYAGAPGRSSVMTWYEM
                     GVEGDERVFATGDCKVVWMDTRTGKSAPLPDALRAAVA"
     gene            15317..16957
                     /locus_tag="PKX01_07345"
     CDS             15317..16957
                     /locus_tag="PKX01_07345"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HMZ75977.1"
                     /translation="MERDSMDFDVLIVGGGPAGLSAAIRIKQIAAEKGQDVSVCLIEK
                     GAEVGAHILSGAVVDPRALNELFPDWQALGAPLTTPVSEDRVIFLSETGGLKVPNAFL
                     PDSFHNAGNYIVRLGNLAKWLGEQAEGLGVEIYPGFAGAQLLTGDNGAVLGVITGDMG
                     LLKDGTEGPNFQPGMELRAKYTLFAEGCRGHLGKELEARFNLREGVDPQTYGIGLKEL
                     WEVPAPQHVQGLVVHTAGWPMEASTYGGGFVYHLEDNLISVGYVVGLNYSNPFLSPFE
                     EFQRFKTHPEIRKFLEGGKRLAYGARAIAAGGLQSQPRLVFPGGALIGDDAGFLNAAR
                     IKGTHAAMKSGMLAAEAVFEAVTAGRAQDSLDAYPAAFRESWLYSELHKTRNFKPYMK
                     KGLWMGSLLFGIDQKVFGGKLPWTLHNHADHTALRPAAECTPIAYPKPDGKLTFDRLS
                     SVFLSNTNHEEDEPCHLRLADASIPISVNLARYDAPEQRYCPAGVYEIVRSDDGAARL
                     QINAQNCVHCKTCDIKDPTQNINWVTPQGGEGPIYQGM"
     gene            17068..18207
                     /locus_tag="PKX01_07350"
     CDS             17068..18207
                     /locus_tag="PKX01_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HMZ75978.1"
                     /translation="MKTAQIVLNGSSVSSSLATLTAIQPHLVFVFADPSWCVGEDRLS
                     PLLQALTPAQLVGCSTAGEVAGHGVQENSCVLTAVHFDDPAFTVTRTHVDDMRDSRAA
                     GERLAVALAPHSPHSVLVFGPGVRLNGSSLVAGMAAGLPGATTLAGGLAGDAGHFRTT
                     WTIGPDGIRERDVVAVGFHSPRLKVCHGSFGGWTPFGPVRRVTRAHENILYELDGERA
                     LDVYRRYLGDYAKDLPASGLLFPFAMLNGDRDETGIIRTILGIDAEAGSLLLAGEIDP
                     HGYLRLMHASTDRLVDGAAEAAARIGKAPQRSDGLALLVSCVGRKLVMGDRVDEEVEA
                     VARVLGPTSCLAGFYSYGEIAPHGADADCTLHNQTMTITRLHEEA"
     gene            18207..21332
                     /locus_tag="PKX01_07355"
     CDS             18207..21332
                     /locus_tag="PKX01_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ75979.1"
                     /translation="MPDVFHPLLLRQLRRTCSLGDAAELETALAELRKASLDGRLDGS
                     AAALASGLERLFERVGGGYRQLERDLDLRTRSLEHSSAELNALNARLQDDLESRSRAL
                     AALRTTLIELQDAPAPPSADASSEGLEATSHLLARLVESRQQKARELDYQKFALDQHA
                     IVVVMNAHGIIEYANDRFCRVSGYQSDELVGRHRHMLTSRDHPRSFFIDLWRAVNGGD
                     VWHGQICNRQRDGRPYWLDTTIVPFRDGGGAISRHIAICTDISAMKQLEQALREQLAL
                     IETLFETIPIPIYIKDAGGHYQRFNKAFEEFFGIRREDWLGRTALEVLPESLARRHVA
                     LDEEVLGCGGVQEFEFEWPFPGGTRVGDYRKVALRDPHGAVSGLIGVIHDITPRKQAE
                     EAMRQARDAAENANRAKSDFLANMSHEIRTPMNGILGMTELTLDTDLNAEQREYLQLV
                     QTSAESLMGIINDILDFSKIEAGKVLVEHIDFDLHRTVTEALKTLAVRAYEKNLELLC
                     DIDPAVPQRISGDPGRLRQVLLNLTGNAIKFTPGGEVAVTVRYAADAGEAGPRIHLAV
                     RDTGIGIAPDKHTAIFEPFSQEDGSTTRRFGGTGLGLTICRRLVEAMGGRLWLDSTPG
                     TGSTFHFTLPAANPAAAPREAPPAVLAGRRVLIVDDHPTNRLVLERSLRRIGMQACST
                     GASDHVVAILNASQCEGTPKEVVLIDSCMPGMDGFTLAQAIVAEGFDPCPALVLLSSA
                     GAKGDAARCREIGIEAYLTRPVSQDEISQTLQRVMAARPRPAPRSLVTRHVLGEDGPR
                     LNLLVLEDQAVSRRLMIALLTRLGHQVELADTLAGIAPAARRFGGPVDVVLASLQVPE
                     LPPSRVIAGLRAAGIDAPVIGVTADPDEDVRNACVAAGIADVLAKPLQTGALRAALDV
                     LAATRCDVRDAARHATAAPRFDYARALLDADREIVDMIGEQLLAQLPGEIAQLRSSLS
                     GAPPLTIQARIRTLKGLLDTVRAEPAIRLLDELALRLSQSDTPYADDLLATLDAEIAE
                     FTRALRARRPG"
     gene            complement(21412..25341)
                     /gene="purL"
                     /locus_tag="PKX01_07360"
                     /gene_synonym="purI"
     CDS             complement(21412..25341)
                     /gene="purL"
                     /locus_tag="PKX01_07360"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HMZ75980.1"
                     /translation="MSEILKLRGAPALSASRLARLTDSVKTVLPRLRGLAAEHWYFVE
                     VSAPLSAGQVERLVDLLGAHPEGDAPSGSLLLVTPRLGTISPWSSKATDIAKQCGFDT
                     IVRIERGTALSFDLRGGLDARARESVLPAIHDRMTESVLDNPDAAHALFHHYTPQPLS
                     TVAVVEQGRDALVVANAALGLALSEDEIDYLVDNFTRVGRNPTDVELMMFAQANSEHC
                     RHKIFNADWIIDGRPEEKSLFGMIRDTHKAHPQGTVVAYSDNASVIEGATVPRLYPDA
                     SGAWIYRDELTHILAKVETHNHPTAISPFPGASTGSGGEIRDEGATGRGSKPKAGLAG
                     FSVSNLEIPGFEQPWEQAYGKPERIASPLDIMIEGPLGAAAFNNEFGRPNLTGYFRTF
                     QQSVQDEVRGYHKPIMIAGGLGSIQAEQSHKVQFGPGTLLIQLGGSGMLIGLGGGAAS
                     SMATGTNTADLDFASVQRGNPEIQRRCQEVIDACWQKGADNPILSIHDVGAGGLSNAF
                     PELADSAGLGARFELREVHIEEPGMSPREIWSNESQERYVLAIAPESLAVFQALCERE
                     RCPFAVVGTASDDGRLVVSDRHFGNAPVDMEMQVLLGKPPKMTRNVSTRQVFVPPFDV
                     TDISLDDACRRVLRMPAVASKNFLITIGDRSVGGMTARDQMVGPWQVPVADVAVTTMS
                     FLGYRGEAFAMGERTPVATLDAPASGRMAIGEAITNLAAADIASLGDVKLSANWMAAA
                     GHRGEDVKLFRTVEAVSRFCQSAGLSIPVGKDSLSMRTAWHDDGEDKQVVSPLSLIVT
                     AFAPVQDVRRTLTPQLQLPEGVETELLLIDLGNHRNRLGGSVLAQAYNSVAEHAPDVD
                     PAQLKAFFDLIQQWRRDDLLLAYHDRSDGGLFATLCEMAFASHCGLSVMLDTVAYDPF
                     MNDVDGLDKRPDALKGRFNDMLFKALFAEELGAVIQIRREDRSRITEPLRQAGLNYHF
                     VGEPNDRDELRFWRNAKLQFAAPRAELHRIWSETSYQIARLRDDATCAQEEFDALAEA
                     GNPGLSVVLGFDPAEDIVAPFIATGARPKVAILREQGVNSQAEMAAAFARAGFAPYDV
                     HMSDLQAGRVHLEDFKGLAACGGFSYGDVLGAGQGWAKSILFNSRLRDQFEAFFQRTD
                     TFALGVCNGCQMMSNLAEIIPGGDCWPRFQRNRSEQFEARFVMVEVVDSPSILLAGMA
                     GSRMPIVVSHGEGRAVFAGIDAQDNARVALRYVDNTGAVAERYPFNPNGSPAGITGLT
                     TPDGRFTIMMPHPERTARTVQMSWHPAGLGEDSPWLRMFRNARRWVG"
     gene            25500..>25930
                     /locus_tag="PKX01_07365"
     CDS             25500..>25930
                     /locus_tag="PKX01_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75981.1"
                     /translation="MSSADPAPSDLPSWPWIALPALILALLAGLMAADGLNRSAFLNL
                     NAAAAGLAPAGFWAGLSVTGSVLGAFAVLAPTLKTRPRWLASALLAAPPALLFSQGGK
                     RLFDEMRPAAEFAAGSYHQIGVRLYVDAFPSGHATTAFVVAA"
BASE COUNT         4515 a         8955 c         8080 g         4380 t
ORIGIN      
        1 cagtacttcg cctacatcga gatcacgccg ggcgaacccc agaacctgtg gcgcgaatac
       61 gaaaagctga ccggcgcgaa atccaacttc gcgatgcagc acatcgaagg cctgccggac
      121 atctaccccg tgttccgcga actcttcaag aagaaactcg catgagcgcc agacgaccca
      181 aacgcaagaa ggccctcccc gctcccggcg aatggacttt cgacctcatt gagacctacc
      241 acgccgagat cgaccgggtg gcgcgggatt tcgggctcga cacctacccc atccagctcg
      301 agatcatcac ctccgagcag atgatggacg cctattcgtc cgtcggcatg cccgtcaatt
      361 accaccactg gtccttcggc aagagcttcc tgcagaccga gaagagctac cgccgcggcc
      421 agatgggcct cgcctacgag atcgtcatca actccaaccc ctgcatcgcc tacctcatgg
      481 aagagaacac catgacgatg caggccctgg tgatcgccca cgcggcctac ggccacaaca
      541 gcttcttcaa gggcaactat cttttccggc agtggaccaa cgccgacgcg atcattgact
      601 atctgctctt cgcgcggaat ttcctgggcg aatgtgagca gcgccacggc gaagaagccg
      661 ttgagctgct gctcgactcc tgtcatgccc tgatgaacgt gggcgtggat cgctacaagc
      721 gccccgccaa gctctccctc gagaaggaac gcacccgcca gcgggagcgc gccgaatacc
      781 tgcagagcca ggtcaacgat ctgtggcgca ccctgcccat tcagcagatc aaggcccagg
      841 ccgtggagga gcgccgcttc ccgcccgagc cccaggagaa cctgctctac ttcatcgaaa
      901 agaacgcccc cctgctcgaa ccctggcaac gggaagtcgt gcgcatcgtg cgcaaggtgg
      961 gtcaatactt tttcccccag cgccagaccc aggtcatgaa cgaaggctgg gccacctact
     1021 ggcactacac cctcctcaac acgctgtacg acgaaggcct gctcagcgac agcttcatgc
     1081 tggaatttct ccactcccac accaacgttg tctatcagcc gcgctacagc gaaccctggt
     1141 accggggtct caacccttac gccctgggct tcgccatgtg gcaggacatc cggcgcatct
     1201 gcgagcaccc caccgacgaa gatcgcgcct ggtttcccga tatggctggc agcgactggc
     1261 gcgagacctt cgatttcgcc atgcgcaact tcaaggacga gagcttcatc gcccagttcc
     1321 tgtctcccaa ggtcacgcgc gacttccgcc tcttcgccat cctcgacaac gacaaggacc
     1381 ccaagctcaa ggtctccgcc atccacgacg aaagtggcta ccggcgcgtg cgtgagatcc
     1441 tctcggacca gtacaacctc gggagccgcg agcccaatat ccaggtgtgg aacgtggacc
     1501 tgcgcggtga ccgctccctc accctgcgcc accaggccta catgcgtcga ccgatggggg
     1561 gcgacgccga ggaggtcgtc aagcatatcg gccgtctgtg gggtttcccg gtacgcctgg
     1621 aaggcgtgca ggacgacggt acggtccaga agctccatga atggtcgccg aaacacgctc
     1681 aggacgcttg atcgccggaa cgcggaaccc ggccactgcg ccaaccgcca tcccgatgat
     1741 ccttagcgtg ccgcaggcgg gtaatatatc cggatgtccg ccgacgactt tcatcgcatc
     1801 ctcggagtca aacccggcgc ttcggccgcc gagatcaaac gcgcctatcg ccgtcaggcc
     1861 atgcgctggc acccggaccg caatgccgat cccgacgccc acgaccagtt caagcgcatc
     1921 cgcgaggcct tcgagcgcct gagcgtcggc gcttcgcagg tcgacaacga cggcgacgcg
     1981 gacaccccac cgcccgccgc gccccgtggc gaggaccgcc gcgtcgaagt caccctcgat
     2041 cttacggaag ccgcactcgg gacgaccgtg gacgtcacgg tcgaagggcg cacatcctgt
     2101 accgcctgtg gcggcagcgg ccaacgcagc tatgggcgta ccaccatgtg cggcgcctgc
     2161 cacggcagcg gccgtgtccg cagcaagaat gggctggaca actgtacgca gtgttccggc
     2221 aaaggctact tcaccgaccg ccgctgcccc gattgcgacg gccgcggctg gcatccatcc
     2281 gagcggcgca tcgaagtcac ggtaccgcca gcgctattgc ccggagatga actccgcgta
     2341 cccggccaag gcggccctgc gcccgaagcc ggcacaccgg gtgatctcta catcaccgta
     2401 cgggcccgac ctcaccccct gttccgtctg gacgggcgga acgtgcatct gacggtcccg
     2461 gtcagcgtct tccgtctgct ggcgggcggt gagatccttg tgcccaccct cagcgggctc
     2521 gaaaccgtgc agcttcccga aggcgccggt cagcccgaac tgcgggttac gggccacggc
     2581 tttccggccc gcggcaagcg ccccgccggc gacctgcacc tacacctcaa gaccgaactg
     2641 ccggcctatc tgagccagga acagaaggcc atgctcgaaa tggccgagca ggttctgctt
     2701 caccacctgg acacccaaag cccggctctt gccctgtgga aacaggtgct tgaacagcac
     2761 cgctgaaccc ggcgcgccac tccgccgaac atcgccggcc cccttcccgg ctgccaccca
     2821 tgcccccgaa aacgcccgaa cttctcgccc cggccggatc cctcgcgatg ctggacaccg
     2881 ccctcgcctt tggtgccacc gccctctacg cgggccagcc gcgctactcc ctgcgggtgc
     2941 gcaacaacga cttcggcgac ctgcccatcc tcgcggaagg catccgccgc acccatgcga
     3001 tcggtgcgcg cttctatctg gtggccaaca tctatccgca cggcgccaag gtgaagacct
     3061 tcctgaagga catcgcgccc gtcatagccc tcgcccccga cgcgctgatc atgtccgatc
     3121 ccggattgat cctcctcgtg cgggagcact tcccccacat gcccatccac ctgtcggttc
     3181 aggccaacac cgtgaacgcg gccgccgtgc gcttctggcg ccaggcaggc atcagccgcg
     3241 tgatcctgtc ccgcgaactc tccctcgatg agatcgcgtc gatccgccaa gactgccccg
     3301 ataccgagct ggaggtcttc atccacggcg ccctgtgcat tgcctattcc gggcgctgtc
     3361 tcctgtcggg ctacttcaac catcgggatc ccaatcaggg cacctgcacc aatgcctgcc
     3421 gctgggagtt caagacccac ggcgcacgta cctgcagcgc cggtgatgtg catctgctcg
     3481 aagagcccca gaagcgcgcc ggcgaataca tgcccattga ggaagacgag cacggcacct
     3541 acatcctcaa ctccagggat ctgcgcgcga tcgagcatgt ggaacggctc gtcgagatgg
     3601 gcatcgactc cttcaagatc gaaggtcgca ccaagagccc ctattacgtg gcccggaccg
     3661 ttcaggccta ccgtcaggcc atcgacgatg ccgttgcggg acggcgtctt gatcccgccc
     3721 tcctcggcca gctcgaaggt ctggccaacc gcgggtatac cgatggcttt taccgtcgtc
     3781 acacccccca tgcaacgcag aattacctgc gcggccactc cgaatcaggg cgtagtctgt
     3841 atgtgggtga tgtggtggcc ttcgatgctg cccgcggcct cgcggaaatc gcttcccgca
     3901 acgccttttc ggtcggagac cggatcgaac tgattcagcc ggccggcaca cgcgaaatcc
     3961 gcatcgaccg tctcgaagat gcagacggtg cgccacttga acgcgtgccc ggcagcgacc
     4021 accgggcctg gattccactg ccgcccgatt gcgtcggtgc ctttgtggcg cgctttatac
     4081 acccctgaac caagaacctt gcataaggcg ctgaaagcgt tcaaaatata gcgttttacg
     4141 ggtggccaaa ttcaccaccc acgtacttta aggaaccgtg atgaaagccg atacccatcc
     4201 caaatacgcc gaagtggaag tgacctgctc ctgtggcaac gtctttacca cccgttccac
     4261 gctgggcaaa cctcagctgc atgtggaagt gtgtgcagcc tgccacccct tctacaccgg
     4321 taagcagaag atcgttgaca ccgcgggtcg cgtcgagcgc ttccgtcaga agtacggtaa
     4381 cgttcaacgt ctgggctgat tcccggcgac gaccgcaaaa ggcagccgag gctgcctttt
     4441 ttattgcccc caagactccg caccgatgcc tgttcctccc aagtctgccc ttttgcccac
     4501 gcctctgacc ggctggccgc ttgcagtcct gatcggcgtc tacctgcttg tcggtatggg
     4561 ccatgttccc tggcggggcg acgacctgac ctatcttggt ccgatccacg ccattctcac
     4621 cgatggctcg tggctgatcc cgagcattgc cggcgagccc ttctacgaat atccacccct
     4681 gtattactgg cttggcgccg tcctcgcacg cgcattcgga tggctgatgc cggttcacga
     4741 tgccgcacgt ctggccagca gcctgtgcgc tgccgccaca ctctactgga ccggtcttgc
     4801 ggcacggcga ctttatggcg acaacacggg tgctccggcg atcctgctcg gacttggcag
     4861 cctcggactg gtggttcacg cccacgaaac gcaacctctc ctggcccaac tggcgggcat
     4921 ggccctgtgt tacgccggcc ttgcagacat ggtcaggcgg ccttgggccg gcgcacgcca
     4981 ggcaggaatc ggcgcaggcc tgacctttct ggccggcgga ctgcccggca tgttcgtcac
     5041 cctgccgctg ttctttgcca tgccgctcct ctgcgacaac tgccggaatg gcgccgtgcc
     5101 gcgggccctg gcaggagggc ttgtcgttgc cctgctgctg ggcggtagct ggcccgcact
     5161 cctgggatgg ctccaccccg aagcccttgc cagctggtgg aacgacgaac tggccgccag
     5221 cctgccccac ggtaatcacc tgcaggccct cggaaagatg atcccgctgc tcggctggtt
     5281 cgcgtggccc ctatggccca ttgcgggctg ggccctgtgg aagaaccgtc gtgccttgaa
     5341 caccccccct gtcgccctgc ccatcatcgc aagtcttctc gcgatggtgg ccgtcgtgac
     5401 gacgggcccg ctccgtcccg cgagcaccct gccgatcctg ccccctcttt gcctgctggc
     5461 ggccctgggc gtcggcaccc tgcgtcgcgg cgccgccaat gccctcgact ggtttgcgat
     5521 catggctttt gcgttcttcg cgatcctggt gtggatcggc tggtccgcgc tgtattttgg
     5581 ctggccgccc gggctggggc gccagttggc acgcctggca cccgatctgg tacgttcccc
     5641 gtcgaccatc gctgccgtga ccgggatgtt cctgagcctc gcgctcttcg cgctaccggt
     5701 gctcacaacc cgctctacgc tccggggggc taccaactgg gccatcggca tgacgctgct
     5761 ctggtgcctg gcggtcgcat tgtggcaacc gtggttcgag cacaccaaaa actacgcgcc
     5821 ggtcgcccag gcgctggccc gcgaactgga gctccaccct gccccttgca tcacgcgcaa
     5881 gggactgaac gacacccagc gcgcggccct cgactatttt aacggcataa aaaccattca
     5941 tcattctacc gaccaagact gtccggcatt tctcgtatat agtgccgacg gcttgccgta
     6001 tcgcccaccc ggcgactggc ggacaagctg ggaaaagcgc ctcggtggag ggcgcaagac
     6061 ggagcttttc agactgtatc ggcatgactg acctgacgcc ttttgatctc gacgcctcgg
     6121 acgcgggaac atccctcagc cgcctggctt ccgcaccaga actgacccat ctgcgcgccc
     6181 ttttctcaaa ggaccctgcg cgcttccagg ccttcagctg tcgctacggc gaatggctgc
     6241 tcgattactc acgtcagcgg atggacgccg ccatccggtc ccagcttctg gcgctggccg
     6301 aggaatcacg gctccgtcaa ggcatcgatg cgatgctgtc cggcgccatt gtcaatgtca
     6361 ccgaacagcg cgccgtactc cattgcgcag cccgcctctc gccccacacc cccctgaacg
     6421 cgcggaatca gatcgaagag acgctggcac gcgtgatggc cctggtcgac cggattcatg
     6481 ccggatcatt gaccggaagc cgcggaatgc cgatccggca tgtggtgcat ctgggaatcg
     6541 gtggctccga cctgggcccg cgcgtgcttt cggaagcctt ctccactgcc tatccgcggc
     6601 gtctcaaagt gtctttcgcg gccaacgtgg atgcgtccga aatccggtcg atcctcatcg
     6661 acgcgactcc ggaggaaacc ctcttcgttg tcgcgtcgaa gagcttcacg acggctgaaa
     6721 ccctcagcaa cgcgcgtatc gcccgtcaat ggctgacgga tcagcttgga cccgatgccg
     6781 atgtccgcgc ccactttgca gccatcagca acaacgtcca tgccgcgtcc tccttcggcg
     6841 tccctgagga acgcatcttc cccttgcccg aatgggtcgg cggacgcttt tcggtgtggt
     6901 cggctatcgg tatcccgatc atgctcgaat tcggaactcc cgtgtttctc gaattcctcg
     6961 cgggtggacg ggacatggac gcgcatttcg caacggcccc atttgactcg aatctcccgg
     7021 tgctcgcggc cctcatcggc ctctggaacg ccagctacct gcgcatcaac tgccacgccg
     7081 tgctgcccta ctgtcacgcc ctgcgcagcc tgccggccta cctgcaacag cttgagatgg
     7141 agagcaacgg caagcacgtc cggcgggacg gtagtgccct gtcgtacccg acggcaccga
     7201 tcctgttcgg cggcgctggc acggtcggcc agcacgccta ccaccagctc ttttatcagg
     7261 gcaccgaaac cgttgccctg gatttcatcg ttccgattcc ggacaccccg acgcctgcaa
     7321 cccgcagcct cgtgggcaac gcgctggccc aggccgcagc gctgatgcac ggacgcacag
     7381 aggatgaagc gcgtgcagaa ctcacgcaac gtgggctcgc acaggatgaa gtcgagcgtc
     7441 tcgcacctca cctggtatgc ccgggcaacc aacccagcag cacgctgctg atgccagccc
     7501 tgaccccctt cacggtcggt cagctcattg cactgtacga gcacaaggtg tttgcccagg
     7561 gctggatgtg gggcctcaac agcttcgacc agtatggcgt cgaactgggc aagcaactcg
     7621 cccgccgtct tgaatccggc gacatgtccg atgcggacag ctccacccag ggcctcatgg
     7681 ccgacatcgc cagacgctgg gaaccggcca aggaatgacg cggccgtacg acaccaaata
     7741 aaaaggcggg tccgacccgc ctttttttcg cacgcgtgtc acgtcattgc ggagtcttct
     7801 gcggcacctg cacatacacc tcgtccggct tgatcaggcc caattcgaag cgcgcacgct
     7861 cctcgactgc ctcgtagccg gacttcagat cccgcacttc cgcttcgagc cccgtgttgc
     7921 gctgctcgag cttgcgattg ccttccttct gctcggcgag ctgctgctcc acttcccaga
     7981 cgcgcaacca gccgcccttg ccgatccaca ggggatactg caaggcggcg acgaggagga
     8041 cgaggatcag cagcggccag cgcatgggag atactccgga tcggcctcac ggccgcgccg
     8101 gagctgaacc gatcaacggg tccgcaggtt gtagaacgcc gacagaccgg ggtagaaggc
     8161 ggtatcgccc agatcttctt cgatgcgcag cagctggttg tacttggaga tgcgatcgga
     8221 gcgggacagg gagccggtct tgatctgcat cgcgttgaga cccacggcga tgtcggcgat
     8281 ggtggagtcc tcggtctcgc cggaacggtg ggagatcacc gcagtgtagc tggcgcgctt
     8341 ggccatctcg acggcggcga aggtttcggt cagggtgccg atctggttga tcttgatgag
     8401 gatggagttg gcgatgccct tctcgatgcc ttccttgagg atgcgggtgt tggtgacgaa
     8461 gaggtcgtcg ccgacgatct gcaccttctt gcccagacgc tcggtcagga tcttccagcc
     8521 ggcccagtca ccttcggcca tgccgtcttc gatggagatg atggggaact tgtcggccag
     8581 ggtggcgagg taatcggtga agccctcgga actgagggac aggccttcgc catgcagttc
     8641 gtacttgcca tccttgaaga actcggacgc ggcacagtcg agggccagca ccacgtcgcg
     8701 gcccggctcg tagccggcgt tggcggtggc ttcgaggatc atgttgaggg cttcgtcggt
     8761 gcccgacaca ttgggcgcga aaccgccttc gtcacccacc gtggtggggt agcccttctt
     8821 gtcggtgagc ttcttcaggt gatggaagat ctcggcgccg cagcgcaggg cttcgcggaa
     8881 gctcttggcg ccgaccggca tgatcatgca ttcctggatg tccagggagt tgttggcgtg
     8941 ggcaccgcca ttgatgacgt tcatcatcgg cacgggcata gacatgccgc cggagccacc
     9001 gaagtagcgg tacaggggca gaccggcctc ttcggccgcc gccttggcca cggccatgga
     9061 gacggccagc atggcgttgg cgcccaggcg cgacttgttc tcggtgccgt ccagttcgat
     9121 cagggtccgg tcgaggaagg cctgctcttc ggcatccagc ccgaccagcg cttcggagat
     9181 ttcggtgttg acgttctcga ccgcttgcag cacgcccttg cccaggtagc gggcggcatc
     9241 cccatcgcgc agctcgatgg cttcgcggga tccggtggat gcaccggacg gcaccgccgc
     9301 gcggcccatc acgccggatt cgagcaggac atcggcttca accgtgggat tgccgcggga
     9361 atccagtacc tcgcgggcaa tgacatcaac aatggcgctc atgaacttcc tttcgagtgt
     9421 gcaaatggcg atataacggg tgcggccgtc ggaccgcgtt caaatttgaa ggatggcggg
     9481 gcggggctgc gctccatcag gcctgcgttt cgatgaagcc ctgccccttg accagcgcat
     9541 cgatatcctt gagggtcttc agcaacgcct tgaggcgcgg caatggccac gcgttgggac
     9601 cgtctgacag agctttggcc gggtccggat gggtttccat gaacaggccg gcgacgccca
     9661 cggccaccgc ggcccgcgcc aggaccggca cgaactcccg ctgaccaccg gacgaggtgc
     9721 cctgcccgcc gggcagttgc accgaatggg tggcgtcgaa caccaccggg cagccggtgt
     9781 cgcgcatgat cgccagggaa cgcatgtcgg acaccaggtt gttgtagccg aaggatgcgc
     9841 cccgctcgca gaccatgatc gtgtctgccc cgccattggc ttcgcgggcc ttgtccacca
     9901 cattcttcat gtcgccgggc gcaaggaact ggcccttctt gatattcaca ggcttgccgg
     9961 aagcggccac cgcgtggatg aagtcggtct ggcggcacag gaaggccgga gtctggagca
    10021 catccaccac ggccgccacg gccgggactt cgtcttcggt gtggacatcg gtgagcaccg
    10081 gcacgccgac cgtggcgcgc acgtcggcga ggatctcgag ccccttctcc atgcccatgc
    10141 cgcggaagga cttgccggaa ctgcggttgg ccttgtcgta gctggacttg tagatgaagg
    10201 gaatggccag ctcggcgcag atctccttga gcgccgacgc ggtgtccagc gccatctcgc
    10261 gggactcgat gacgcacggt ccggcgatga ggaaaaacgg cttatccaga ccgacatcga
    10321 atccgcagag tttcatgggg tttcctttca gccggcgtgc tggtgggcca gggccgcctt
    10381 gacgaatgca gtgaacagcg gatggccctg acgcggattg gaggtgaatt ccggatggaa
    10441 ctgacagccc acaaaccacg ggtgggcgtt cgggccttcc gtcggcagct cgatcatctc
    10501 gcacagatca gtgcccggcg cgcgtccggc gacccgcaga ccctgcgctt ccagacggct
    10561 caacaggcta ttgttcactt cgtagcgatg acggtgacgc tcggtgatct cgggccgacc
    10621 atagacttcc cgcgccaatg aaccttcggt caagcggcag acctgaccgc ccagacgcat
    10681 ggtgccgccc aggtcggaat cctcgccacg cttttcaagc ttgccggaag cgtccagcca
    10741 ctcggtgatg aggccgatca ccgggaattc cgtgtgcttg tcgaattcag tgctgtgggc
    10801 accgttcatg ccggccacat cacgggcgaa ctcgacgaca gcgagctgca tcccgaggca
    10861 gatgcccagg taaggcacct tgttctcgcg ggcgtagcgg atcgcggcga tcttgccttc
    10921 ggtgccgcgc cgaccgaacc cgccgggcac gagaatcgca tccatgcgct tgagcacgtc
    10981 gcacccgctc ttctcgatgt cctcggagtc gatgtagtgg atgctcacct tgctgcgggt
    11041 gtgcatgccc gcgtgattca gcgcctcgat gagggacttg taggactcgg tgagatccac
    11101 gtacttgccg acaaaggcga tgtccaggtg atgctggggg ttgttgagcg catcgatgag
    11161 gcgctcccac acagacagat ctgccgcgcg ggccaggatg cccagcttgt ggcagacgat
    11221 ctcgtcgagc atctggtcat ggagcatgcc cgggatgctg tagatggaat ccgcatccag
    11281 acactcgatg accgcttcgg gcatcacgtt gcagaacagc gcaatcttgc gccgctcgtc
    11341 ggcggggatg gaacggtcgg cgcggcacag caggatgtcg ggctggatgc cgatctcgcg
    11401 gagttccttg acggagtgct gggtcggctt ggtcttcagc tcgcccgcgg tggggatgta
    11461 gggcagcagg gtcaggtgga tgaagcaggt gccctgacga ccttcctcga tacccatctg
    11521 acggatggct tccaggaaag gcagcgattc gatgtcgccc accgtgccac ccacctcgac
    11581 gatggccacg tccgcccctt cggcgccgcg cttgatgtag agcttgatct cgtcggtgat
    11641 gtgggggatg acctgaaccg tcttgccgag gtattcgccg cggcgttcct tcttgatgac
    11701 ggcttcgtag atctggccgg tggtgaagtt gttgcgcttg cccatcttgg cgctggtgaa
    11761 gcgctcgtaa tggcccagat ccagatcggt ctcggcgcca tcttcggtca cgaacacttc
    11821 cccgtgctgg aagggactca tcgtgccggg gtcgacgttg atgtaggggt cgagcttgag
    11881 gtgggtaacc ttgatgccgc gggattcgag aatggccccg agggaggcgg cggcgatgcc
    11941 cttgcccaga gaggacacga caccaccggt aacgaagacg tatttggtca tgacacgggg
    12001 gacttctgga aagagaaatt atagccgatc cacccacccc gctcaacggt ggatgcctcc
    12061 ccagccgtcc cccgaaacgc tgcccgtacg cctccgtcag cggatgaaat tccggctgac
    12121 cgtccggaat tccggtgccg tgcagcgctc catccattcg aacgccacca tctcgcgact
    12181 caccacttcg ataccgtggg cccgcatgcg ggccagggca agttccttgt cagcaggctt
    12241 tcgcgacccg accgcctcgg ccacgacgaa cacctccttg ccctgccaac gcagcccgag
    12301 ggcagtctga agcacacaga catgtgcctc ggtgcccgtc acaaccacct gacgacgggc
    12361 atcgaccggc gtgccgctca gactcccgtc cgccacacag gagaaggccg tcttgtacac
    12421 cacggccgcg tcgcctgcag cctcctgcaa ggcggtcgcc gttggtccga gcccctgcgg
    12481 atattgctct gacagcacga ccggcacgcc cagttgacgg gcgacacgga cgagccagac
    12541 gcaatggtcg agtacggcgg cgccatcgtc gatcgcgggc aacagacgct cctgaacatc
    12601 cacgaccagc aggctggcgc aacgacgatc catcagcatg gcggactcct cagttcacca
    12661 gttccgggcg cgaccggaaa ttgtccaggg cggcggggtt ggcgagcgcc tcgagattct
    12721 tcaccggttc cccatgcacg acggcacgca cggccagttc gacgatcttg ccgctgcggg
    12781 tacgcggaat gtcgtccacc tggacgatgc gcgccggcac atggcgcggc gtcgcgttgt
    12841 cccggatggt ctgacggatc tgttcgcaca gggcgtcgtc caggacccgc ccctcccgca
    12901 gcttgacaaa caacaccacg cgcacgtcgc cgctgcccgc tggccactgc tggccgatga
    12961 cgagggattc gagcactgcg tcgcactttt ccacctggcg gtagatttcg gcggtgccga
    13021 tgcgcacgcc gccgggattg agggtagcgt cggagcgacc atagatgatc agccccgcgt
    13081 gggcggtaat ctcacaatag tcgccgtggc accacacgtg ctcgaaacgc tcgaaatacg
    13141 cggcacggta gcgactcccg tccgggtcgt tccagaatcc gacgggcatg gacgggaagg
    13201 gcgcggtgca gaccagttcg cctttctccc cctgtattgg cgtgcccgtc tcgtcgaaaa
    13261 cgtctaccgc catgcccagc ccgcggcact ggatctcgcc cggatacacg ggcagtgtcg
    13321 gattgcccag aacgaagcac gagatgatgt ccgtcccgcc tgagatggat gacaggcaca
    13381 ggtccgcctt gaccgcccga tacacgtact cgaagccttc cggcaccagc gggctgcccg
    13441 tcgagaacat cgcccgcagg gacgtgaggt cgtgcgtctc ccgcggcgcc accccgagct
    13501 tggcgatgcc gtcgatgaac ttggccgacg tgccgaagtg ggtcatgcgc tccgcctcgg
    13561 cgaaatcgaa caggatgctg ccctgcccca ccatcggcga accgtcgtac agcagcagcg
    13621 tcgcgcctga agccaggcca ctcaccagcc agttccacat catccagccg caggtggtga
    13681 agtagaacaa gcggtcgccg ggccggacat cgccatgcag gcggagctcc ttgatgtgct
    13741 gaagcagggt gccaccggca ctatggacga tgcacttggg cacccccgtc gtgcccgacg
    13801 aatacaggat gaacaaggga tgggcgaagg gcagccggac gaagggaatc tccgtctccg
    13861 ccaggaatgg ccgcacgaat tcgggcagca gacgggcatg atggatgtgg gagagatcgt
    13921 ggctcgcatg gacgtagggc accaccacca cccgccgcag ggacggcaga ccatcggcga
    13981 tggcggccag gcggtcgaga caatccacca ccttgccgtt gtagtaatag ccatccaccg
    14041 ccaccagcac cttgggctcg atctgcccga agcggtcgag caccccctgc acgccgaaat
    14101 ccggcgacgc ggacgagaag actgcgccga tgctcgccgc cgccagcatg atgacgatcg
    14161 tctccggaag attcggcatc cacgccgcca cccggtcacc cgcgcccacg ccctgctcct
    14221 gcaaggccgc ggcaaagtgc gccacctgtc ggtacagatc gccgtgggac agccggttct
    14281 tgaccttgtc ttctccccag aacaccagcg catcggcgtc gtcgcggctg cgcaacaggt
    14341 tctcggcgta gttcaggcgg gcatcgggaa accagcgggc tcccggcatc cgctcgccgt
    14401 cggcgagcac ggtgtcaccg cgcactccgc acacgtcgcc gtcctcccag accgaggtcc
    14461 agaactcctc tggatgggcg acagaccagg catgcaacgc cgcgtagcca tcaagcggcc
    14521 gctgccaacg ggcagacgcg cgctgcgcga atccggtgat caacgccgcg gccacgcgct
    14581 cgggcgacgg cgtccacagg ggcgcggcag caatcggata actcatggcg aaactcctct
    14641 tcctcgaacg ggaatggatc gacggaacag gccctcaggc caccgcggca cgcagcgcat
    14701 ccggcaaggg cgcggacttg ccggtacgcg tatccatcca caccaccttg cagtcgcctg
    14761 tggcaaacac gcgttcatca ccctccacgc ccatctcgta ccaggtcatc acgctgctgc
    14821 gtcccggcgc gccggcatac agacgcacga tcacggtggc aggatagttc accgggatca
    14881 aaaaggtaca actcgcgtta atgatcaccg gcgcggtctc ggcggtgggc cccacggcaa
    14941 atccgaacgc ttccagccac tccacccggg cctgctcgta atagcggaaa tacaccgtgt
    15001 tattgacgtg cccgtagaaa tccatgtctc cccagcgtac ggggatgcgg gtggtgagca
    15061 gaagtttgcg atcaggcgca gaggcgggtg tgttcatggg ctgtctcctg tgggtttgct
    15121 ggattcgttg tcgtggccac ccgcccatca cgcccaggcg ggggcacgag gaatttagca
    15181 tacggtggcg tatgcccaac acaccggagg gcgtcgggca ggcttttttg tgacgccggg
    15241 attgccccgt cctgcgcccg gatgtacgct cgacgtctaa cgaaaacaag acataccaaa
    15301 acaaaaggag ggcagtatgg aacgcgattc catggacttt gacgtcctga tcgtgggggg
    15361 agggcctgcc gggctgtccg ccgcgatccg gatcaagcag atcgcagcag aaaaggggca
    15421 ggacgtctcc gtctgcctca tcgagaaggg cgccgaggtc ggcgcccaca ttctctccgg
    15481 cgcggtggtc gacccccgcg ccctcaatga actcttcccg gactggcagg ccctcggcgc
    15541 accgctcacc acgcccgtga gcgaggaccg ggtaatcttc ctgtccgaaa ccggcggctt
    15601 gaaggtgccc aacgccttcc tgccggacag ttttcacaac gccggcaatt acatcgtccg
    15661 cctcggcaat ctggccaagt ggctggggga acaggccgaa ggcctcggcg tcgagatcta
    15721 tccgggcttt gccggcgccc agttgctcac cggtgacaac ggcgcggtgc tgggcgtcat
    15781 caccggcgac atgggcctgc tcaaggacgg gaccgaaggc cccaacttcc agccgggcat
    15841 ggagctgcgc gcaaaataca cgctgttcgc cgaaggctgc cgtggccacc tgggcaagga
    15901 actcgaagcg cgcttcaatc tgcgcgaagg cgtcgacccc cagacctacg gcatcggcct
    15961 gaaggaactg tgggaagttc ccgcgcccca gcatgtgcag ggtcttgtgg tgcacaccgc
    16021 aggctggccg atggaggcat ccacctatgg cggcgggttc gtctaccacc tggaagacaa
    16081 tctcatctcc gtcggctacg tggtcggcct caactacagc aatcccttcc tgtcaccctt
    16141 cgaggaattc cagcgtttca agacccatcc tgagatccgc aaattccttg aaggcggcaa
    16201 gcgcctcgcc tacggcgccc gagccatcgc ggccggcggc ctgcagagcc agcccaggct
    16261 ggtcttcccg ggcggtgccc tgatcggtga cgatgccggc ttcctcaacg cggcccgcat
    16321 caagggcacc cacgccgcca tgaagtccgg gatgctcgcc gccgaggcgg ttttcgaggc
    16381 cgtcaccgcc ggtcgcgccc aggacagcct cgacgcctat cccgccgcct tccgcgagag
    16441 ctggctgtat tccgagttgc acaagactcg caacttcaag ccctacatga aaaaagggct
    16501 gtggatgggc tccctgctct tcggtatcga ccagaaggtc ttcggcggca agctgccatg
    16561 gaccctccac aaccacgccg atcacaccgc cctgcgaccg gccgccgaat gcacgccgat
    16621 cgcctatccc aaaccggacg gcaagctgac cttcgaccgc ctctcctcgg tgttcctgtc
    16681 caacaccaac cacgaggaag acgaaccctg ccacctgcgt ctggcggatg cctcgattcc
    16741 catctcggtc aacctggccc gctatgacgc ccccgagcag cgctactgtc cggctggcgt
    16801 ttacgagatc gtacgcagcg acgacggtgc cgcgcgcctg cagatcaacg cccagaactg
    16861 cgtccactgc aaaacctgcg acatcaagga tccgacccag aacatcaact gggtcacgcc
    16921 tcaaggcggc gaaggcccga tctaccaggg catgtagcgc gcccgtcgtc cagcgggcgt
    16981 agcaccctgc gcccgcccca cctcaagtca tcatggggca ggccgttagg gcggatatcc
    17041 ttttggtccg tcccacgacc tgccaccatg aagactgccc agatcgtcct gaacggatcc
    17101 tccgtcagtt ccagcctcgc gaccctcacc gcgatccagc cccacctcgt atttgtgttc
    17161 gccgacccgt cctggtgcgt cggcgaagac cggctctcgc cgctcctcca agccctgacg
    17221 cccgcgcagc tggtcggctg ctccacggcg ggcgaagtgg cgggccacgg cgttcaggaa
    17281 aactcctgcg tgctgaccgc ggtccatttc gacgacccgg cgttcaccgt cacccgcacg
    17341 cacgtggacg acatgcgcga ctcccgcgca gccggtgaac ggctggcggt cgccctggcg
    17401 ccccactcgc ctcattccgt actggtcttc ggtcctgggg tgcgcctcaa cggctcgtca
    17461 ctggtggccg gcatggcggc aggattgcct ggcgccacca cactggccgg cggtctggcg
    17521 ggcgatgcgg ggcattttcg taccacctgg accatcggac cggatggcat ccgcgagcgg
    17581 gacgtggtcg ccgtggggtt tcacagcccc cgcctgaagg tctgccatgg aagcttcggt
    17641 ggctggacgc cctttggacc cgttcgacgg gtcacgcggg cccatgaaaa catcctgtac
    17701 gaactggacg gagagcgcgc cctcgatgtc tatcggcgct acctgggcga ctacgccaag
    17761 gacctcccgg cgtccggcct gctgtttcct tttgcaatgc tcaacggtga ccgggatgaa
    17821 accggtatca tcagaaccat tttgggtatc gacgccgagg cgggcagcct gttgctggcc
    17881 ggagaaatcg atccgcacgg ctacctccgt ctcatgcacg ccagcacgga tcgcctggtg
    17941 gacggcgccg ccgaagcggc cgcccggatc ggtaaagcgc cccagcgcag cgatggactg
    18001 gccctgctgg tgagttgcgt gggccgcaaa ctggtcatgg gcgaccgggt ggacgaggag
    18061 gtcgaagccg tcgcccgtgt cctcggtccg acaagttgcc tcgcgggctt ctattcctat
    18121 ggtgaaatcg caccccacgg cgcggacgcg gactgcaccc tgcacaatca gaccatgacg
    18181 atcacccgcc tccacgagga ggcctgatgc ctgacgtatt ccatccgctg ctcctgcgtc
    18241 agctccggcg cacgtgcagc ctgggcgatg ccgcggaact tgaaaccgcc cttgccgagc
    18301 tgcgcaaggc cagcctggat gggcgcctgg acggcagcgc tgcggccctg gctagtgggc
    18361 tcgaacgcct gttcgagcgg gtgggcggcg gctaccgtca gctcgagcgc gacctcgacc
    18421 tgcgcacccg cagcctcgaa cacagctctg ccgaactcaa tgccctcaac gcccggcttc
    18481 aggatgatct ggaaagccgc tcgcgcgccc tcgccgcgct gcggaccacc ctgatcgaac
    18541 tccaggacgc accggcgccg ccgtcagcgg acgcctcctc cgaaggtctc gaagccacct
    18601 cccacctcct cgcccgcctc gtcgagagcc gtcagcagaa ggcacgggag ctggactacc
    18661 agaaatttgc cctcgaccag cacgccatcg tcgtggtcat gaacgcgcac ggcatcatcg
    18721 aatacgcgaa tgaccgcttc tgccgggttt ccggctacca gtccgacgaa ctggtgggcc
    18781 gccaccggca catgctcacc agccgcgatc acccccgcag cttctttatc gacttgtggc
    18841 gcgccgtcaa tggcggcgac gtctggcatg gccagatctg caaccggcag cgggacggcc
    18901 gcccctactg gctcgacacc accatcgtcc ccttccgcga cggcgggggc gccatctccc
    18961 gtcacatcgc catctgtacc gacatcagcg ccatgaagca gctcgaacag gcgctgcggg
    19021 agcagctcgc gctgatcgag acgctcttcg agaccatccc gataccgatc tacatcaagg
    19081 acgcgggcgg gcactaccaa cgtttcaaca aagccttcga ggaattcttc ggcatccgcc
    19141 gcgaggactg gctgggccgg accgcactcg aagtgctgcc cgagtcgctg gcccggcgcc
    19201 atgtcgctct ggacgaggag gtgctgggat gtggcggcgt gcaggagttc gagttcgaat
    19261 ggcccttccc cggcggcacg cgggtgggcg actaccgcaa ggtggccctg cgcgaccccc
    19321 acggagccgt cagcggcctg atcggcgtga tccatgacat caccccgcgc aagcaggccg
    19381 aggaggccat gcgccaggcc cgcgatgccg ccgaaaacgc caatcgggcc aagagcgatt
    19441 tcctggccaa catgagccac gagatccgta cgcccatgaa cggcatcctc ggcatgaccg
    19501 aactcaccct cgacaccgat ctgaacgcgg agcagcggga gtacctgcag ctcgtgcaga
    19561 cctccgccga atccctgatg ggcatcatca acgacatcct cgacttctcc aagatcgagg
    19621 ctggcaaggt gctcgtcgag cacatcgatt tcgacctgca tcgcaccgtc accgaagccc
    19681 tcaagacgct cgcggtgcgc gcctacgaaa aaaacctcga actgctctgc gacatcgatc
    19741 cggcagtgcc ccagcgcatc tccggcgatc ccggccggct gcgtcaggta ctgctcaacc
    19801 tgaccggcaa tgccatcaaa ttcacccctg gcggtgaagt cgccgtcacg gtgcgctacg
    19861 ccgcggacgc cggcgaagcc ggtccgcgca tccatctggc cgtacgggat acgggcatcg
    19921 gcatcgcacc ggacaaacac actgccatct ttgagccctt cagccaggaa gacggctcca
    19981 ccacccgccg cttcggcggc accggcctgg gcctcaccat ctgccgccgc ctcgtggaag
    20041 ccatgggcgg gcggctgtgg ctggacagca cacccggcac gggcagcacc tttcacttca
    20101 ccctcccggc ggccaatccg gcggcagcgc ctcgggaggc cccgccggcg gtactggccg
    20161 gcagacgggt cctgatcgtc gacgaccacc cgaccaatcg gctcgtgctt gaacgcagcc
    20221 tgcggcgcat cggcatgcag gcctgcagca ccggcgccag cgatcacgtt gtggccatcc
    20281 tgaacgccag ccagtgcgag ggcaccccca aggaggttgt cctcatcgac agctgcatgc
    20341 cgggcatgga cggcttcacg ctggctcagg ccatcgtcgc cgaaggcttc gatccgtgcc
    20401 ccgccctggt gctgctgtcc tcggccggcg ccaagggcga tgccgcccgc tgtcgcgaga
    20461 tcggcatcga agcctatctc acccggccgg tcagccagga cgaaatcagc cagaccctgc
    20521 agcgggtcat ggccgcccgc ccgcgccccg cgccccggtc gctggtcacc cgccatgtgc
    20581 tcggcgagga cggcccccgc ctcaacctgc tggtgctgga agaccaggcc gtgagtcgcc
    20641 ggctgatgat cgccctcctc acgcgcctcg ggcatcaggt cgagctggca gacacgctgg
    20701 cgggcatcgc ccccgctgcg cgacgtttcg gcggaccggt ggatgtcgtg ctggccagcc
    20761 tgcaagtgcc ggaactgccc cccagccgcg tgatcgccgg cctgcgggcg gccggcatcg
    20821 acgcgcccgt gattggtgta accgcggatc cggacgaaga cgtgcgcaac gcctgcgtcg
    20881 ccgccggcat cgccgacgtc ctcgccaagc ccttgcagac cggcgccctg cgcgcagccc
    20941 tggacgtcct cgcggcaacc cgctgcgatg tccgggacgc ggcccgccat gcgaccgcag
    21001 cgccccgttt cgactacgcc cgcgccctgc tggacgccga ccgggagatc gtggacatga
    21061 tcggcgagca gttgctggcg cagctgcctg gcgagatcgc ccagctgcgc agcagcctgt
    21121 ccggcgcgcc gcccctcaca atccaggcca ggatccggac cctgaagggt ctcctcgaca
    21181 ccgtgcgggc cgaaccggcc atccgtctcc tcgacgagct ggccctacgc ctgagccagt
    21241 ccgacacccc ctacgcagac gacctgctgg ccaccctgga cgccgagatc gcggaattca
    21301 cgcgagccct gcgcgcccgc cgcccgggct gagggtccgg cgcgccccgc gaacgcctcc
    21361 acagcgcgcc tgcatcagcc cggaggccgg cgcgcggacc accgaccggt ctcagcccac
    21421 ccagcggcgc gcgttgcgga acatccgcag ccagggcgaa tcctcaccca gaccggccgg
    21481 atgccagctc atctgcaccg tgcgggcggt gcgttccggg tgcggcatca tgatcgtgaa
    21541 gcggccgtcc ggcgtggtca ggccagtgat gccggccggc gaaccgttgg gattaaaggg
    21601 ataacgctcg gccaccgcgc cggtgttgtc cacgtagcgc agggccacgc gggcgttgtc
    21661 ctgggcgtcg atgcccgcga agacggctcg tccttcgccg tgggacacca cgatgggcat
    21721 gcggctgccg gccatgccgg cgagcaggat ggacgggctg tccaccacct ccaccatcac
    21781 gaagcgggcc tcgaactgct cgctgcggtt gcgctggaag cgcggccaac agtcgccgcc
    21841 gggaatgatc tcggccaggt tggacatcat ctggcagccg ttgcagacgc ccagggcgaa
    21901 ggtgtcggtg cgctggaaga aggcctcgaa ctggtcgcgc aggcggctgt tgaagaggat
    21961 ggacttggcc cagccctgcc cggcgccgag cacgtcaccg taggaaaagc cgccgcacgc
    22021 ggcgaggccc ttgaagtctt ccagatggac gcgcccggcc tgcaggtcgg acatgtgcac
    22081 gtcgtagggc gcaaagccgg cccgcgcgaa ggcggccgcc atctcggcct gggaattgac
    22141 gccctgttcg cgcaggatcg ccaccttggg acgcgcgccg gtggcgatga agggcgcgac
    22201 gatgtcctcg gccggatcga agccgaggac cacgctcagg ccggggttgc cggcctcggc
    22261 cagggcatcg aactcctcct gggcgcaggt agcgtcgtcc cgcaggcggg cgatctggta
    22321 gctggtctcg gaccagatcc ggtgcaactc ggcccgcggc gcggcaaact ggagcttggc
    22381 gttgcgccag aagcgcagct cgtcccggtc attgggctca ccgacgaagt ggtaattgag
    22441 gccggcctgg cgcaggggct cggtgatgcg gctgcggtct tcgcggcgga tctggatcac
    22501 ggcgcccagt tcctcggcga agagggcctt gaagagcatg tcgttgaagc gccccttgag
    22561 ggcgtccggg cgcttgtcga ggccgtccac gtcattcatg aacgggtcgt aggccaccgt
    22621 gtcgagcatc accgacaaac cgcagtggga cgcgaaggcc atttcacaca gggtggcgaa
    22681 gaggccgccg tcagagcggt cgtggtaggc cagcagcagg tcgtcccggc gccattgctg
    22741 gatcaggtcg aagaaggcct tgagctgggc cggatccacg tccggcgcgt gttccgccac
    22801 cgagttgtag gcctgggcca gcaccgagcc gccgaggcgg ttgcggtggt tgcccagatc
    22861 gatgagcagc agctcggtct cgacgccttc cggaagctgg agctggggcg tcagggtgcg
    22921 gcgcacgtcc tgcaccggcg cgaaggcggt gacgatcagg gacaagggcg agacgacctg
    22981 cttgtcctca ccgtcgtcgt gccaggcggt gcgcatggac agggaatcct tgcccaccgg
    23041 aatcgacaga ccggcgctct ggcagaagcg ggagaccgcc tcgacggtgc ggaacagttt
    23101 gacgtcctca ccgcgatggc cggcggcggc catccagttg gcggagagct tcacgtctcc
    23161 gaggctggcg atgtctgccg cggcgaggtt ggtgatcgcc tcgccgatag ccatgcgacc
    23221 ggaggccggc gcgtccagcg tggcgaccgg ggtgcgctcg cccatggcga aggcctcgcc
    23281 gcgatagccc aggaagctca tggtggtgac ggccacgtcg gccaccggca cctgccacgg
    23341 gccgaccatc tggtcgcggg cggtcatgcc gcccacggaa cggtcgccga tggtgatgag
    23401 gaagttcttg ctggccaccg ccggcatgcg cagcacgcgc cggcaggcgt cgtccaggct
    23461 gatgtcggtc acgtcgaagg gcggcacgaa gacctggcgg gtggacacgt tgcgggtcat
    23521 cttgggcggc ttgccgagga gcacctgcat ctccatgtcc accggcgcgt tgccgaaatg
    23581 ccggtccgac accaccaggc gaccgtcgtc cgacgccgtg cccaccaccg cgaaggggca
    23641 acgctcccgc tcgcagaggg cctggaagac cgccaggctc tccggcgcga tggccagcac
    23701 gtagcgctcc tgggattcgt tggaccagat ctcccgcggg ctcatgcccg gctcctcgat
    23761 atgcacctcg cgcagttcga agcgcgcgcc aaggccggcg gaatcggcca gctcggggaa
    23821 ggcattggaa aggccgccgg cgcccacgtc gtggatggac aggatcgggt tgtcggcccc
    23881 cttctgccag caggcgtcga tgacctcctg gcagcgccgc tggatttccg ggttgccgcg
    23941 ctgcaccgag gcaaagtcca ggtcggcggt gttggtgccg gtcgccatgg atgaagcggc
    24001 gccgccgccc aggccgatga gcatgcccga gccgcccaac tggatcaaca gcgtgccggg
    24061 gccgaactgc accttgtggg actgctcggc ctggatgctg cccaggccgc cggcgatcat
    24121 gatgggcttg tggtagccac gcacctcgtc ctgcacgctc tgctggaagg tgcggaaata
    24181 gcccgtcagg ttgggacggc cgaactcgtt gttgaaggcc gccgcgccca gcgggccttc
    24241 gatcataatg tcgaggggcg acgcgatgcg ctccggcttg ccgtaggcct gctcccaggg
    24301 ctgctcgaag ccggggatct ccaggttgga caccgagaaa ccggccagac cggccttggg
    24361 cttggagcca cggccggtgg cgccctcgtc gcggatctcg ccgccggagc cggtggacgc
    24421 gccggggaag ggcgagatgg cggtcgggtg gttgtgggtc tccaccttgg cgaggatgtg
    24481 ggtcagctcg tcccggtaga tccacgcgcc ggaggcgtcg ggatacaggc gcggcacggt
    24541 ggcgccctcg atcaccgagg cgttgtcgga ataagctacc acggtgccct gcgggtgggc
    24601 cttgtgggtg tcgcggatca tgccgaagag ggatttctcc tcgggccggc cgtcgatgat
    24661 ccagtcggcg ttgaagatct tgtgccggca gtgctcggaa ttggcctggg cgaacatcat
    24721 cagctccacg tccgtcgggt tgcggcccac acgggtgaag ttgtccacca gatagtcgat
    24781 ctcgtcctcg gacagggcca gacccagtgc agcgttggcc accaccagcg cgtcgcggcc
    24841 ctgctcgacc accgccacgg tgctcagggg ctggggcgtg taatggtgga acagggcgtg
    24901 ggcggcgtcc gggttgtcca gcaccgactc ggtcatgcgg tcgtggatgg ccggcagcac
    24961 ggactcccgc gcacgggcgt cgaggccgcc gcgcaggtcg aagctcaggg ccgtgccccg
    25021 ctcgatgcgc acaatggtgt cgaagccgca ctgcttagcg atgtccgtcg ccttcgacga
    25081 ccagggcgag atcgtgccca ggcgcggcgt caccagcagc agactgcccg acggcgcatc
    25141 cccctcggga tgggcgccga gcagatccac cagccgctcc acctgcccgg cggacagggg
    25201 cgccgacacc tccacgaaat accagtgctc ggccgccagc ccgcgcaggc gcggcaggac
    25261 ggtcttcaca gagtcggtga ggcgggcaag gcgggaggcg gacagggcgg gcgcaccgcg
    25321 cagcttgaga atttcggaca tggcaaggat ggcgcgtagt tggaaccgac ccggcgcgac
    25381 ggccgggaca cacaggaccg ggcattatac ccgagccccg ccccgcgcct tgcgacgcat
    25441 gtccgccttg cccccggctt cgggtacact cccgcctttt gctgccccac ccgcgcctgt
    25501 tgagctcagc cgaccccgct ccctccgacc tgccgtcctg gccctggatc gccctgccgg
    25561 cgctgatcct cgccctgctg gccggactga tggcggcaga cggcctcaat cgcagcgcct
    25621 tcctgaatct caacgccgcg gcagccgggc tggcgcccgc cggattctgg gccggcctgt
    25681 ccgtcaccgg ctcggtgctc ggcgcctttg ccgtcctcgc ccccaccctc aagacccgcc
    25741 cgcgctggct cgcctcggcc ctgctcgccg cgccgccggc gctcctcttc agccagggcg
    25801 gcaagcgcct ctttgacgag atgcgccccg ccgccgaatt cgcagccggc agctaccacc
    25861 agatcggcgt gcggctttac gtggacgcct tcccgtccgg ccacgccacc accgccttcg
    25921 tcgtcgccgc
//
LOCUS       DAOQPA010000038        25707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_892_length_25707_cov_15.871160, whole genome shotgun sequence.
ACCESSION   DAOQPA010000038 DAOQPA010000000
VERSION     DAOQPA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 25707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25707
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_25707_cov_15.871160"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1102)
                     /gene="pstS"
                     /locus_tag="PKX01_07370"
     CDS             complement(62..1102)
                     /gene="pstS"
                     /locus_tag="PKX01_07370"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HMZ75982.1"
                     /translation="MLNAVTRSLLASAGAVLASTAFAADITGAGASFPAPVYAKWADA
                     YQKATGNKVNYQSIGSSGGIKQITAKTVDFGASDMPLSPEQLEKDGMQQFPTVIGGVV
                     PVVNIPGVQPGQLKLTGEILANIYLGKITKWNDPAIVALNTNLNLPASDIGVVRRADG
                     SGTTFIFTNYLSKVSADWKNQVKEGATVQWPLGLGGKGNEGVSAFVQRLPGAIGYVEY
                     AYAKQNKLTFASLKNKDGAFVLPDDENFKAAAAGADWSKNAFYEILTDKPGKDSWPIT
                     GATFILMHKVQDKPVQGGEALKFFEWAYHNGAKLATDLDYVPLPESTVKLIRASWAGI
                     KDASGKAVFSGK"
     gene            1433..2851
                     /locus_tag="PKX01_07375"
     CDS             1433..2851
                     /locus_tag="PKX01_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HMZ75983.1"
                     /translation="MRHISLLAAAVSAALLAGCGGNSSTQETPATNVIFFLGDGMGIN
                     TLTAARIYKVGEDGDLTIDTLPETAFVRTYSNNAQVTDSAPSMAAYMTGVKMNNEVIS
                     MSADTMAFDASGNNYLSGTDSTCPASGNGSAVTTLLELAKKAGWSTGAVTTTRVTHAT
                     PAVTYSHICHRDAENTIAAQMVPGGSGYNSALGDGLNVLMGGGRQFYLPTASGGKRTD
                     GRDLTAELVKQGYTYVKNKTEFDAAAAASTDKLVGLFTSSHMSYDIDRDPTVEPSLAE
                     MTTKAIDILSKNKKGFFLMVEGGRIDHALHETTAAKALQDTVAFDDAIQTALTKMKAV
                     DPTLANTLIVVTADHDHTLVLNGYAKRTGATTASNPGILGLLKNFVSGANATDSGGLP
                     FTILGFGNGENRPATRSALTDAQVSVKTYHQEAAVPMAAGSETHGGTDVFLGAIGRGA
                     DKFTGVITNTEVFGLIRSAVGL"
     gene            2872..4281
                     /locus_tag="PKX01_07380"
     CDS             2872..4281
                     /locus_tag="PKX01_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HMZ75984.1"
                     /translation="MKFQIKTCVAALALVGTTSAFAAGEATNVIFFLGDGMGPTTVTA
                     SRIYKYGEAGKLNMESLTRTARIKTYSNDAQTTDSAPSMAAYMTGVKMNNEVLSMSAD
                     TKAYASTATNSGYVSGADSTCPATGNGTAVTTLLELSKAAGKSVGAVTTTRITHATPA
                     ATYSHICHRDGENDIAAQAVPGSGKFNSALGSGVDVLLGGGWRFFKPNTVTGGSRTDG
                     QDLTAKLQTAGYTYVETGTAFKAVDATATSKLVGLFNRDHLNYELDRVNKSIDEPSLA
                     DMTEKAIKILQKNGKGFFLMVEGGRIDHALHGTNAKRALKDTIAFDEAIKRALSLVDL
                     KNTLVVVTADHDHTMTFNGYSHKGNPILGKVTDITQTTVKKTPVLAAAADGKPYTTLV
                     FGNGGGPRKATRDDLTSVDTTTDDYLQEVAVNLGSAGTETHGGGDVMLFSGGAGSSGF
                     KGTMVNTKVFGLVKSALGL"
     gene            4389..5054
                     /locus_tag="PKX01_07385"
     CDS             4389..5054
                     /locus_tag="PKX01_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75985.1"
                     /translation="MKKLLLAALLGALTAGARADDLALRIEHDSTVVGADGVTRNVRF
                     AERLIRRDAQSWLARILPPGAHEDAEHAAGAKGHKHMDIAAATRWVVRAPDGKLRVRL
                     VNDHDRMIVDIAPVDYANIGFDGRWSTAAQLIDPEQIRQMKPLARSAPAGTRWYESGT
                     PDARVQVLWDEKDKYPRRIESSNAAGTRRSVLNATREALPAVLPWTRLDGYALKEYSD
                     LLD"
     gene            5130..5534
                     /locus_tag="PKX01_07390"
     CDS             5130..5534
                     /locus_tag="PKX01_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ75986.1"
                     /translation="MESIRLQGVPMVFFELFSNAPDIVRVPAGKALFHEGEEGCCMFV
                     LTIGSAEVYVKNRRVELLVPGNMVGELALVTRGPRTATVLALEDSEFVSIDEDRFNYL
                     VQHTPFFALNVMRLLAERLTAASSALPAVEDV"
     gene            complement(5545..5751)
                     /locus_tag="PKX01_07395"
     CDS             complement(5545..5751)
                     /locus_tag="PKX01_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ75987.1"
                     /translation="MSDPRLNHPTCAECAYFQSNGATSGTCHRYPPSFAGEHPAEHHT
                     WRFPAVTSFSWCGEFAPDGNAGPR"
     gene            5959..>6305
                     /locus_tag="PKX01_07400"
     CDS             5959..>6305
                     /locus_tag="PKX01_07400"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HMZ75988.1"
                     /translation="MPRPRLRALRTAAFLALLSLGLSACSPRQLVVRSVADQLAGQDA
                     GSENDPELAREASAFYLKLSESVLRQDPDHLPLATAVAGGFTQYAYAFVAFDADRLEA
                     RDSRGAQALRERAA"
     assembly_gap    6306..6405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6406..7013
                     /locus_tag="PKX01_07405"
     CDS             <6406..7013
                     /locus_tag="PKX01_07405"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HMZ75989.1"
                     /translation="RLEARDSRGAQALRERAARLYRRARDHALAALERHRPGLAAELA
                     TAGPERPLAKDLVPLAYWGAAAWGGLVSLSKDDPDTVADLPRAIRLARLAWLADPAWG
                     DGTLASLMGSFEAARPGGSARQAEAYFDDALRLAAGRSAGAFVAKAEGVAQPAGDKAA
                     FLDLLRQAEAVRDTPGSPLTLANEVMRRRARWLAENADDLF"
     gene            7050..8072
                     /gene="dctP"
                     /locus_tag="PKX01_07410"
     CDS             7050..8072
                     /gene="dctP"
                     /locus_tag="PKX01_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HMZ75990.1"
                     /translation="MPLTARRHALRLLATLPALALPGLAVAAGPQMRIGTLAPKNSLY
                     HRQLLEIGEAWRAAQGEGAKFTVYPDGSQGGETDMARRMRIGQLQGALLSVVGLREIE
                     PSISALQNMPLMFRSWDEVDYVREKIRPAMEKKFLDKGFVVLTWGDAGWVRFFSKEAA
                     VRPDDYKKMKFFAWAGESEQQDIMKSLGYTPVPLETSDILPSIQTGMINAVPSTPYFA
                     LASQVYATANHMLDINWAPIVGALVVTKKAWDDMTPAAQTALRDAAAKAGAQMRQQAR
                     KEVDEAVEAMKKRGLVVHTPTPEQMKEWNDLADKLYPRIRGKMVPADTFDEVVSLLKT
                     YRAGKR"
     gene            8144..9961
                     /locus_tag="PKX01_07415"
     CDS             8144..9961
                     /locus_tag="PKX01_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HMZ75991.1"
                     /translation="MRRFLPDEALASAALFLMTLIPLVEMAVRPLFGKGIDNAPLLVQ
                     HLGLILAMFGALLAERGGHLTSLGNSLGSARDPRVRAAAGLFAHAAAALLCGLLGAAS
                     WAFVASELDAGRELAYGLPVWTVQAAMPLGFLLLGMRLGARCAPAGLARPLVGASLAG
                     LGFLLARHFDGSTLSFAPFAVALLAALLAGAPIFAVLGGLALGLFWSEGQPLASVPLS
                     HYQITVNPSLPALPLFTLAGLVFARSGAAGRLGTLFTACFGGGATGSAVAAAVLCSCF
                     TAFTGGSGVTILALGGLLLPLLRDAGLPERRGISLVTSASALGVLLAPSVPLIMVAII
                     ARVPINTMFLAGLLPALVMVLCLLLVGGYLRSTPRAGAPVAAPSAGVPPGVGLRAAIW
                     AARWEILAPVVAIGALASGIATPTESAALTAAYALFTQAVLHREVDATLLRRCLADCA
                     QVIGGVMLILGMALGLTNYLVDAGIPDAAVDWVQATLPNKFAFLVALNLFLFVAGALM
                     EIYAAIVVLVPLLLPVAVSYDIDPVHFGVIFLAVMEMGFLCPPAGMNIFFASAMFARP
                     IREVAVSVLPALLAIFLGALIIAWVPWLATGLPALFALH"
     gene            complement(9983..10678)
                     /locus_tag="PKX01_07420"
     CDS             complement(9983..10678)
                     /locus_tag="PKX01_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ75992.1"
                     /translation="MGTDPRPVVVVIEDEPKIRRFVCMALESADLQAFEADSVKRGLI
                     EAGTRQADLVVLDLGLPDGDGKAFIRELRTWSAIPVIVLSARTQESEKVAALDAGADD
                     YLVKPFGAAELLARVRAQLRRAAPRDGTPGVMSFGRLRADFGRRLIERDGVPVHLTPI
                     EFRLLACLASHPDTVLTHRHLALTVWGPGHAEDVHYVRVHMAQLRKKIEDDPSRPRHL
                     LTESGVGYRFCPR"
     gene            complement(10668..13391)
                     /gene="kdpD"
                     /locus_tag="PKX01_07425"
     CDS             complement(10668..13391)
                     /gene="kdpD"
                     /locus_tag="PKX01_07425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HMZ75993.1"
                     /translation="MDDPRRDPQRILDRLREAEGRAARGRLKIFFGASAGVGKTYAML
                     CAAHQQRAQGVDVVVGVVESHGRRETAALLDGLDVLPPRSVAYKGRTLAEFDIDGALA
                     RQPALILVDELAHSNVEGSRHPKRWQDVEELLDAGIDVYTTVNVQHLETLNDVVGGIT
                     GVRVWETVPDHVFDAADEVVLVDLPPDELLQRLKEGKVYLPQQAERAIQNFFRKGNLI
                     ALRELALRRTADRVDSEMVRYRRDSAVSTVWATQESLLACVGPGEGGDRVVRACARLA
                     GRLEVPWHAIYVETPDLRHLSERNRQRILKNLHLAQESGAETASLAGADAAERIAAYA
                     RDHNLARIVVGRDHPRGWRPWHRSMAEQIGRQAPDLDVILVARDEARRDAAVEAPTSE
                     SWLDRLRAPWTAFAMTAALGALATVVATLLLGHFDLANIAMVFLLAVVLTAVRYGLGP
                     AVVASFLNVAAFDYFFVPPRFSFAVSDVQYVLTFAVMLASGLITANLTAGLKLQARVA
                     SRREYRIRALYEMSRDLSGALMPEQIAEIALRFSRTTFAARCTLVLADDANRLLAPLA
                     VPGGLSGVDAGICQWAFDHEAEAGCGTDTLPASPLLYVPLKAPMRIRGVLVLEPRHPG
                     RMGAPEQHRLLETFARLIAISLERVHYIQVAQATTLQMESERLRNSLLSAISHDLRTP
                     LSALVGLVDMMLLTRPAPAPALVQLAESMRDEVQRMNALVSNLLDMARLQAGTVTLNK
                     QWQPLEEVVGSALKGLESALFRHPVEVRLPDDLPLLEFDTVLIERVLSNLVENAVKYT
                     PPGTPVRIGAEGPAGGFVTIWVEDDGPGLPAGREEAIFKKFERGQSESATPGVGLGLA
                     ICRAIVAAHGGTIRAENRADGGARFLFSLPAGQPPAVPGMGGLAEDAPHGH"
     gene            complement(13443..13826)
                     /locus_tag="PKX01_07430"
     CDS             complement(13443..13826)
                     /locus_tag="PKX01_07430"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HMZ75994.1"
                     /translation="MAGRAATAARGGDLGRWVGALVCLGVLGWGGAVQAAGYFRSDAD
                     LQELESVLRSRVPVGTALFVKGYVLGVADAGSGTQWCLPADLTEEHLFQAVARHLPKD
                     PPPRTSRSAAQQVSEALSSEFPCAR"
     gene            complement(13858..14445)
                     /gene="kdpC"
                     /locus_tag="PKX01_07435"
     CDS             complement(13858..14445)
                     /gene="kdpC"
                     /locus_tag="PKX01_07435"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HMZ75995.1"
                     /translation="MNTIVRPALMLCGLLSLLTGLAYPAVVTAVAGWVFPAQAAGSLI
                     LADGAVAGSRLIGQVFEDPRHFWGRPSATAPQPYNGAASSGSNLGPLNPALAEAVQAR
                     VQRLRAADPGNPAPVPADLVQASGSGLDPHISPAAAAYQIGRIARLRGIAPAALESLV
                     AAHTEGRQWGLFGEPRVHVLALNRALDERFPVEGR"
     gene            complement(14458..16524)
                     /gene="kdpB"
                     /locus_tag="PKX01_07440"
     CDS             complement(14458..16524)
                     /gene="kdpB"
                     /locus_tag="PKX01_07440"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HMZ75996.1"
                     /translation="MTRKTLSMFDPDLLRPALVDAFRKLNPAVQWRNPVMFVVYGGSL
                     LTTVLGIQALAGDGEAPAGFIFTVALWLWFTVLFANFAEALAEGRSKAQAAALRGLKQ
                     DTPAKRLTAPRYGAPWQQVSATALRRGDVCLVEAGETVPADGEVIEGVASVDESAITG
                     ESAPVIREAGGDFSALTGGTRLLSDWLVLRVTVNPGETFVDRMIAMVEGARRQKTPNE
                     IALTILLVALTLVFLVVTVTLLPFSVFSVQMAGAGAPVTLTVLVALLVCLIPTTIAGL
                     LSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFIPAEGV
                     TEQALARAAQLASLADETPEGRSIVVLAKQRFNLREHDLQALGAQFVHFSAQTRMSGV
                     DLGGTRIRKGAGEALRRHVEALGGHFPASVTAIVEETARRGSTPLVVAEDARVLGVVE
                     LKDIVKGGIRERFGELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLAL
                     IRQHQAEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSNPTKLIE
                     VVETGKQMLMTRGALTTFSIANDVAKYFAVVPAAFVSTYPQLEALDVMALASPASAIL
                     SAVIFNALIIIVLIPLALRGVRYRPQGAGLLLRRNLTIYGLGGLLAPFVGIKLIDMAL
                     AASGLA"
     gene            complement(16538..18328)
                     /gene="kdpA"
                     /locus_tag="PKX01_07445"
     CDS             complement(16538..18328)
                     /gene="kdpA"
                     /locus_tag="PKX01_07445"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HMZ75997.1"
                     /translation="MSSHAWVLLGLYLLVLLAAAVPLGRYIAAVAEGQGGRALRPGRP
                     IEALIYRLCGVRAEQEMGWRTYALAILAFNLVGALAVYALQRLQDGLPLNPQGLAAVS
                     PDSAFNTAISFATNTNWQGYAGESTMSPLTQMLGLSVQNFLSAATGMAVALALIRGFA
                     RHSAATVGNAWVDLTRMVLYVLLPLSGVVAVFLVGQGVVQDLGGDRLATTLELTHYDA
                     PRLDATGKPLIDASGRVLTEPARTQTQVLSLGPVASQEAIKMLGTNGGGYYNANSAHP
                     FENPTPLSNFVEMLLIFLIPAALCVSFGRMVGDARQGRAILATMTLLFVAMAVAGAHA
                     EAQGHPLLASLGIEGAAGNLEGKETRFGSDDSALFAVITTLASCGAVNGMHDSFTPLG
                     GLVTLWNMMLGEVVFGGVGSGLYGMLVFAVLAVFIAGLMIGRTPEYLGKKIEVHDMKM
                     VSIAILVTPVLVLAGTAVAVLVPAGGAGVGNPGAHGFSEILYAFTSAANNNGSAFAGL
                     SANTPFYNSVLGVVMGLGRFGVIVPVLALAGHLAAKKRLAPGAGTMPTHGPLFVVLLI
                     GTVVLVGALNYVPALALGPVVEHLALYAAH"
     gene            complement(18325..18417)
                     /gene="kdpF"
                     /locus_tag="PKX01_07450"
     CDS             complement(18325..18417)
                     /gene="kdpF"
                     /locus_tag="PKX01_07450"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HMZ75998.1"
                     /translation="MSAMLTVSAVLAAGLLVYLVVALLRPEDFS"
     gene            complement(18641..19555)
                     /gene="lepB"
                     /locus_tag="PKX01_07455"
     CDS             complement(18641..19555)
                     /gene="lepB"
                     /locus_tag="PKX01_07455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ75999.1"
                     /translation="MFDVSPRQPLRAALMSLVLPGYGQFYNGELDKAVWLFLAFAFLC
                     VPGMALVGLYLPPAWTMPALGVSVVAALGTWGYAVVDAWRGARARPDYLPRPWQTGAA
                     YAVAFLVCNGLALPLLVDTVRSRQMESFSIPSASMEPSVLRGDILFADKRYNCPGCKQ
                     QIRRGDVAVFTYPNDRTRLYIKRIVGLPGDRVETRGAAVWVNGVALSTDPVMSGAVTE
                     TLDGRRWQVLRGGTGSTRAPEEAPPDVVLTVPPGQVFVLGDNRDATTDSRRFGPVPMA
                     DVAGRARQVWWSSGPEGVRWARLGAVVE"
     gene            complement(19575..19874)
                     /locus_tag="PKX01_07460"
     CDS             complement(19575..19874)
                     /locus_tag="PKX01_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76000.1"
                     /translation="MRTDPFDRPADAVLDPAVEALLREDFPAPPADFAARVMAALPAV
                     PQPIPPSRAPTGAPAGRGRPFARAAAWLTAVGAGLFGVAQLLSLAFAVWSVTTAG"
     gene            complement(19864..20412)
                     /locus_tag="PKX01_07465"
     CDS             complement(19864..20412)
                     /locus_tag="PKX01_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ76001.1"
                     /translation="MDTPDLLARLRSGDRQAFAALVVRYQRPLFGFLGRMGCAPALAE
                     DLAQDAFVRAWQQLDRYRPEMGALSTWLFTIARHLAINELGRSHHVRERPAAEDAPEP
                     ACPRPQPPEVLALAQQRQRLHAALRQLSLADRSLLALAYVEELAMADIARIEGCSVAA
                     AKTRLTRARQRLGRLLEPSDAY"
     gene            20773..20961
                     /locus_tag="PKX01_07470"
     CDS             20773..20961
                     /locus_tag="PKX01_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76002.1"
                     /translation="MLTQPIRSKQAVAAEREWAGQARRSSTPDQHAAPPTPSAGRGKL
                     WLLLLAAALIAPKLLHYF"
     gene            complement(21017..23227)
                     /gene="katG"
                     /locus_tag="PKX01_07475"
     CDS             complement(21017..23227)
                     /gene="katG"
                     /locus_tag="PKX01_07475"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HMZ76003.1"
                     /translation="MSTASQCPFHAAHGTRATTGARSNRDWWPNQLNLSILHQHGPAS
                     NPLGADFDYAAAFRQLDYAALKADLHTLMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RTADGRGGAGSGNQRFAPLNSWPDNGNLDKARRLLWPIKQKYGNRISWADLMILAGNV
                     ALESMGFKTFGFGGGRADIWAPEEDIYWGAEKTWLATSDQPESRYSGPRDLENPLAAV
                     QMGLIYVNPEGPDGQPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDP
                     KWVGPEPEGAPLEAQGLGWINAFGRGTGGDTTTSGIEGAWKPNPTTWDGGYFDMLFGY
                     EWALTRSPAGAQQWVAQDVKPEHLIPDAHDPAKKHPPMMTTADLSLRFDPIYAPIARR
                     FQQDPAAFADAFARAWFKLTHRDMGPRARYLGPEVPAEDLIWQDPLPARDHPLIDAAD
                     IAGLKARVLQAGLGVAELVSTAWASASTFRGSDLRGGANGARIRLAPQKDWAVNQPPQ
                     LARVLATLEGIQRDFNAAQTDGRQVSLADLIVLAGNAAVEAAAQAGGVTVSVPFAPGR
                     VDARADQTDVDSFAVLEPQADGFRNYQAQSFRVPAEALLVDKAQLLTLSAPEMTVLVG
                     GLRVLGANVGGAPHGVFTSRPGVLSNDFFVNLLDMGTVWAPTAETGETFTGRDRHSGA
                     LRWTATRVDLVFGSNSQLRAIAEVYAQADAAARFAGDFAAAWGKVMDLDRFDLK"
     gene            23553..>25707
                     /locus_tag="PKX01_07480"
     CDS             23553..>25707
                     /locus_tag="PKX01_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76004.1"
                     /translation="MPPTRPPAATSPGKDARPRTPTPAVGSALPARRLALCACLGLGA
                     AIAHAGGVQTLDVVEVTASATDLVGVAGAASEGTVTAKHLANRPLLRPAEVLEAVPGL
                     VISQHSGDGKANQYYLRGFNLDHGTDFATTLLGMPINLPTHAHGQGYSDLQFLIPELV
                     DTVRYRKGPYAADVGDFGSAGSAAIDYVRRLPQAFVELTAGSYGYRRGLVAGSPAVAD
                     GHLLAAFEWTGHDGPWAVPEDLRKLNGLLRYSDGTRSNGWSATALAYSARWNATDQVP
                     LRAVQSGLIGRYGSLDPTDGGTTQRTSLSVEASRQDADGWLRGNAYLIDYRLQLWSNF
                     TSCTLGCAPGPGDQFEQEDRRRIGGFNLAHTWYLNSIARGADFTLGVQSRVDDIGRVG
                     LYTTTARQRWGSVARDSVRQGSLGLYGEATVQWLEKLRSVAGLRADRYRFDVTADTPA
                     NSGQVDAALASPKLALILGPWARTEVYLNFGHGFHSNDARGITLTTQPDFRDPAFGTP
                     AHASTPLVRTKGSEIGVRSALLPGLHSSLALWQLDIASELVFTGDAGTTEARFPSRRR
                     GIEWANDWTPLTGLIVDADFSVSRARYTAVDAGVPGDRIPGALEQVASVGVAWDAGGP
                     WSGGARLRYVGPRPLIEDNTVRAPGSTLVNLRASHRIDPRTRISVDLLNAFNRKVSDI
                     DYYYASQLRGEAAPVDDLHTHPAEPRSLRVSLRVGF"
BASE COUNT         4057 a         9872 c         8083 g         3595 t
ORIGIN      
        1 cgaatggggc cggagagtcg cctcgtccgg ccccctgact ggctgtgcga accttcccgt
       61 cttacttgcc ggagaagacc gccttgccgg aggcatcctt gatgccggcc caggaggcac
      121 ggatcagctt gacggtggat tcgggcaggg gcacgtagtc cagatcggtg gccagcttgg
      181 caccgttgtg gtaggcccac tcgaagaact tgagcgcttc accgccctga accggcttgt
      241 cctggacctt gtgcatcagg atgaaggtgg cgccggtgat gggccagctg tccttgcccg
      301 gcttgtcggt caggatctcg tagaaggcgt tcttgctcca gtcggcgccg gcggcggcgg
      361 ccttgaagtt ctcgtcgtcc ggcagcacga aggcgccgtc cttgttcttc agggacgcaa
      421 aggtcagctt gttctgcttg gcgtaggcgt actccacgta gccgatggcg cccggcagac
      481 gctgcacgaa ggcggacacg ccttcattgc ccttgccgcc caggcccagc ggccactgca
      541 cggtggcgcc ttccttgacc tggttcttcc agtcggcgct gaccttggac aggtagttgg
      601 tgaagatgaa ggtggtgcca gagccgtcgg cgcgacgcac cacaccgatg tcgctggcgg
      661 gcaggttcag gttggtgttg agggcgacga tggccgggtc gttccacttg gtgatcttgc
      721 ccaggtagat gttggccagg atctcgccgg tcagcttgag ctggccgggc tgcacgcccg
      781 gaatgttcac cacgggcacc acgccaccga tcacggtggg aaactgctgc atgccgtcct
      841 tctcgagctg ctcgggggac aggggcatgt cggaggcgcc gaaatcgacg gtcttggcgg
      901 tgatctgctt gatgccgccg gaagagccga tggactggta gttcaccttg ttgccggttg
      961 ccttctggta ggcgtccgcc cacttggcgt agaccggggc ggggaaggaa gcacccgcgc
     1021 cggtgatgtc ggccgcgaag gcggtcgagg cgaggacggc acccgcggaa gcgagcaggg
     1081 aacgggtcac ggcgttcagc atgaagaatc tcctgagtct ggggggtggg atccggggca
     1141 gcggggcggg acgttctctg tggcaccgca caaaacactt gccctgatct cgcgaccgaa
     1201 gcttagattt tgaatgtgac gggattgtga caattcacaa tccggcaacg gcgtgaccgg
     1261 aacacgtccc tcgatcccgc ccgatcggtt gcggcggcgc aacaccctat cactttctgc
     1321 atagcttcct gccagagaat tcaacgcccc ctccgtcaca gcggcgcaac agagcgatgg
     1381 gattatttac atccggttcc atatttcacc ccccgctttc cggagactct tcatgcgaca
     1441 catcagcctt ctggccgcgg ccgtttctgc ggccctactg gccggctgcg gcggcaacag
     1501 cagcacccag gagacgcccg ccaccaacgt catcttcttc ctcggtgacg gcatgggcat
     1561 caacacgctc accgcggcgc gcatctacaa ggtgggtgaa gacggcgacc tgaccatcga
     1621 caccctgccc gagacggcct tcgtccgcac atactccaac aacgcccagg tcactgactc
     1681 ggccccctcc atggccgcct acatgaccgg cgtgaagatg aacaatgaag tcatctccat
     1741 gtccgccgac accatggctt ttgacgccag cggcaacaac tacctgagcg gcaccgacag
     1801 cacctgtccg gccagcggca acggcagcgc cgtcaccacc ctgctggaac tggccaagaa
     1861 ggccggctgg agcaccggcg cggtgaccac cacccgcgtc acccacgcca cccccgcggt
     1921 cacctactcc cacatctgcc accgcgacgc cgagaacacc atcgccgccc agatggttcc
     1981 gggcggcagc ggttacaaca gcgccctggg cgacggcctc aacgtcctga tgggcggcgg
     2041 ccgccagttc tatctaccca ccgcctctgg cggcaagcgt accgacggcc gtgacctgac
     2101 cgccgagctg gtcaagcagg gttacaccta cgtcaagaac aagaccgaat tcgatgccgc
     2161 cgccgcggcc tccaccgaca agctggtggg cctgttcacc tccagccaca tgagctacga
     2221 catcgaccgc gaccccaccg tcgagcccag cctggccgag atgaccacca aggccatcga
     2281 catcctgtcc aagaacaaga agggcttctt cctgatggtg gaaggcggcc gcatcgacca
     2341 cgccctgcac gagaccaccg ccgccaaggc cctgcaggac accgtggcct ttgacgacgc
     2401 catccagacc gccctcacca agatgaaggc cgtcgatccc accctggcca acaccctgat
     2461 cgtcgtcacc gccgaccacg accacaccct ggtgctcaac ggctacgcca agcgcaccgg
     2521 cgccaccacc gccagcaacc ccggcatcct cggtctgctg aagaacttcg tcagcggcgc
     2581 caacgccacc gacagcggcg gcctgccctt caccatcctg ggcttcggca acggcgaaaa
     2641 ccgcccggcc acccgcagcg ccctcaccga cgcccaggtc tccgtcaaga cctaccacca
     2701 ggaagccgcc gtccccatgg ccgccggcag cgaaacccac ggcggcaccg acgtcttcct
     2761 cggcgccatc ggccgcggcg ccgacaagtt caccggcgtc atcaccaaca ccgaagtctt
     2821 cggcctgatc cgcagcgcgg tcggtctgtg agccgcgaca ggagcccttc catgaaattc
     2881 cagatcaaga cctgcgtcgc cgccctcgcc ctggtcggca ccacctccgc ctttgccgcc
     2941 ggcgaagcca ccaacgtcat cttcttcctc ggcgacggca tgggccccac caccgtcacc
     3001 gccagccgca tctacaagta tggcgaggcc ggcaagctga acatggagtc cctgacccgc
     3061 accgcgcgga tcaagaccta ctccaacgac gcccagacca ccgacagcgc cccgtccatg
     3121 gccgcctaca tgaccggcgt caagatgaac aacgaagtgc tgtccatgag cgccgacacc
     3181 aaggcctacg ccagcaccgc caccaacagc ggctacgtca gcggcgccga cagcacctgc
     3241 ccggccaccg gcaacggcac ggccgtcacc accctgctgg agctgtccaa ggccgccggc
     3301 aagtccgtcg gcgcggtgac caccacccgc atcacccacg ccaccccggc ggccacctat
     3361 tcccacatct gccaccggga cggcgagaac gacatcgctg cccaggccgt gcccggctcc
     3421 ggcaagttca acagcgccct cggcagcggc gtggatgtgc tcctcggcgg cggctggcgc
     3481 ttcttcaagc ccaataccgt caccggtggc agccgtaccg atggccagga cctgaccgcc
     3541 aagctgcaga ccgcgggcta cacctacgtg gagaccggca ccgccttcaa ggccgtcgat
     3601 gccaccgcca ccagcaagct ggtcggcctc ttcaaccgcg accacctcaa ctacgagctg
     3661 gatcgggtca acaagagcat cgacgaaccc agcctcgccg acatgaccga aaaggccatc
     3721 aagatcctgc agaagaacgg caagggattc ttcctgatgg tggaaggcgg ccgcatcgac
     3781 cacgccctgc acggcaccaa cgccaagcgc gcactgaaag acaccatcgc cttcgacgaa
     3841 gccatcaagc gcgccctgtc cctggtggat ctgaagaaca ccctggtcgt cgtcaccgcc
     3901 gaccacgatc acaccatgac cttcaacggc tactcccaca agggcaaccc catcctgggc
     3961 aaggtcaccg acatcaccca aaccaccgtc aagaagaccc cggtcctcgc cgccgcggcc
     4021 gacggcaagc cctacaccac cctggtgttc ggcaacggcg gcggcccccg caaggccacc
     4081 cgggacgacc tgaccagcgt ggacaccacc accgacgact acctccagga agtcgcggta
     4141 aacctgggct cggccggcac cgaaacccac ggcggcggcg acgtcatgct gttctccggc
     4201 ggcgccggca gcagcggctt caagggcacg atggtgaaca ccaaggtgtt cggcctggtg
     4261 aagtccgccc tcggcctgta aagcatcgat tgccatccgg gatcggcctg ctgcgtagaa
     4321 gaagttggtc ccatttcccg cccgacccca ccggtcgggc ggcttttttt gagacattcc
     4381 ccgtcacgat gaagaagctc ctcctcgccg ccctcctcgg cgccctcacc gccggcgccc
     4441 gtgccgacga cctggccctg cgcatcgaac acgacagcac cgtcgtcggt gccgacggcg
     4501 tcacccgcaa cgtgcgcttc gccgaacgcc tgatccgccg cgacgcgcag agctggctgg
     4561 cgcgcatcct gccccccggc gcccacgagg acgccgaaca cgccgccggc gccaagggcc
     4621 acaagcacat ggacatcgcc gccgccaccc gctgggtggt ccgcgccccc gacggcaagc
     4681 tgcgggtgcg cctggtgaac gaccacgacc ggatgatcgt ggacatcgca cccgtcgatt
     4741 acgccaacat cggcttcgac ggccgctgga gcaccgcggc ccagctcatc gacccggagc
     4801 agatccgcca gatgaagcct cttgcccgca gcgccccggc cggcacccgc tggtacgaga
     4861 gcggcacgcc cgacgcccgc gtgcaggtac tgtgggacga aaaggacaag tacccgcgac
     4921 gcatcgaatc cagcaacgct gccggcaccc gccgcagcgt cctcaacgcc acccgcgagg
     4981 ccctgcccgc cgtgctgccg tggacgcgcc tggacggcta cgccctgaag gaatactccg
     5041 acctgctcga ctaggaccgg cgccgccgcc cgaggcgccc cctgtcacga atctgtcaca
     5101 cccctggaac cccggcgccg caccatggcg tggaatccat ccgtctgcaa ggtgtcccca
     5161 tggtcttctt cgaactcttc tccaacgccc ccgacatcgt ccgcgtccct gccgggaagg
     5221 ccctcttcca cgaaggtgaa gaaggctgct gcatgttcgt gctgaccatc ggcagcgccg
     5281 aggtctatgt gaagaaccgc cgggtcgagc tcctcgtgcc gggcaacatg gtcggcgagc
     5341 tggccctggt cacccgcggc ccccgcacgg ccaccgtcct cgccctggaa gacagcgagt
     5401 tcgtgtccat cgacgaggac cgcttcaact atctggtcca gcacacgccc tttttcgccc
     5461 tcaacgtcat gcgcctgctg gccgagcgcc tgactgcggc gtccagtgcc ctgccggcgg
     5521 tggaggacgt ctgacccgcg ggcctcagcg cggaccggcg ttcccgtccg gcgcaaactc
     5581 cccgcaccag gaaaagcttg tcaccgccgg aaagcgccag gtgtggtgct ccgccgggtg
     5641 ctcgccggca aaggatggcg ggtagcggtg gcaggtgccg ctggtggccc cattgctctg
     5701 gaagtacgcg cactcggcgc aggtgggatg gttcagtcgg ggatcggaca tggtcgggtc
     5761 gggaaaatgt tgatcgcggc cgtcgccgcg gcgataacgc tgccgctcca ttatgagggc
     5821 ccgatgtgac agaaattcgg cactgcagca tcgccacatc accgtaacgc gcccgtcacc
     5881 cggccgcaac atggcgccgc tatcgtcgcg gcatttccgc acggccggcc ccgaccggcg
     5941 ccgccccgaa ccgtcgccat gccccgtccg cgtctccgcg ccctgcgcac cgctgcattt
     6001 ctcgccctcc tgtccctcgg cctcagcgct tgctccccgc gccagctggt ggtgcgcagc
     6061 gtcgccgacc agttggccgg ccaggacgcc ggcagtgaga acgatccgga gctcgcccgg
     6121 gaagccagcg ccttttacct caagctgtcc gaatccgtgc tgcgtcagga tccggaccac
     6181 ctgcccctcg ccaccgcggt ggccggcggc ttcacccagt acgcctacgc cttcgtcgcc
     6241 ttcgacgccg accgcctcga agcccgcgac agccgcggcg cccaggccct gcgcgagcgg
     6301 gccgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccgc ctcgaagccc
     6421 gcgacagccg cggcgcccag gccctgcgcg agcgggccgc gcgcctctac cggcgcgccc
     6481 gcgaccacgc cctcgccgcc ctcgaacgcc atcgccccgg cctcgccgcc gaactggcca
     6541 cagcgggccc cgagcgtcct ctggccaagg atctcgttcc cctcgcctac tggggcgcgg
     6601 cagcctgggg cggactcgtc agcctctcca aggacgaccc ggacaccgtc gccgacctgc
     6661 cccgtgcgat ccgcctcgcc cgcctggcct ggctcgccga tcccgcctgg ggcgacggca
     6721 cgctggccag cctgatgggc agtttcgagg ccgcccgccc cggcggcagc gcccgccagg
     6781 ccgaagccta tttcgacgac gccctgcgcc ttgccgccgg tcgcagcgcc ggtgccttcg
     6841 tcgccaaggc cgaaggcgtc gcccagcccg ccggcgacaa ggccgccttc ctcgacctgc
     6901 tgcgccaggc cgaggccgtg cgcgacaccc ccggcagccc cctcaccctc gccaacgaag
     6961 tcatgcgccg ccgcgcccgc tggctcgccg agaacgccga cgacctgttc tgaacgtcgg
     7021 cccctcgccc ctcacccgga aaacgcccca tgccgctcac cgcccgccgc cacgccctgc
     7081 gcctgctcgc caccctgccc gccctggccc tgcccggcct cgccgtagcc gccggcccac
     7141 agatgcgcat cggcaccctc gcccccaaga actccctcta ccaccgtcag ttgctggaga
     7201 tcggcgaagc ctggcgcgcg gcccagggcg aaggcgccaa gttcaccgtc tatcccgacg
     7261 gcagccaggg tggcgagacc gacatggccc ggcgcatgcg catcggccag ctccagggcg
     7321 ccctgctgtc ggtggtcggc ctgcgggaga tcgagccctc catctctgcc ctgcagaaca
     7381 tgcccctgat gttccgcagc tgggacgagg tggattacgt ccgcgaaaag atccgccccg
     7441 ccatggaaaa gaagttcctc gacaagggct tcgtcgtcct cacctggggc gatgccggct
     7501 gggtgcgctt tttctccaag gaagccgccg tgcgcccgga cgactacaag aagatgaagt
     7561 tcttcgcctg ggccggcgaa tccgagcagc aggacatcat gaagagcctc ggctacaccc
     7621 cggtgcccct cgaaaccagc gacatcctgc cgtccatcca gaccggcatg atcaacgccg
     7681 tcccgtccac gccctacttc gccctggcca gccaggtcta tgccaccgcg aaccacatgc
     7741 tggacatcaa ctgggctccc atcgtcggcg ccctggtggt gactaaaaag gcctgggacg
     7801 acatgacccc cgccgcccag accgccctgc gcgacgccgc tgccaaggcc ggcgcccaga
     7861 tgcgccagca ggcccgcaag gaggtggacg aagccgtgga ggccatgaag aagcgcggcc
     7921 tggtggtcca cacccccacc cccgagcaga tgaaggaatg gaacgacctg gcggacaagc
     7981 tctatccccg catccgcggc aagatggtgc ccgccgacac cttcgacgag gtcgtgtccc
     8041 tgctcaagac ctaccgcgcc ggcaaacgct gaaggacgtc atggcgcgcc ggccgtgccc
     8101 accttcgtgc tttccgcctc ccacttccgc gccctccccc ccaatgcgcc gcttcctgcc
     8161 cgacgaggcc ctcgcctccg ccgccctctt cctgatgacc ctcatcccgc tggtggagat
     8221 ggccgtgcgg ccgctcttcg gcaagggcat cgacaacgcc ccgctgctgg tccagcacct
     8281 ggggctgatc ctcgccatgt ttggcgccct gctggcggag cggggcggcc acctcacctc
     8341 cctcggcaac agtctgggca gcgcccgcga tccgcgcgtc cgcgctgccg ccggcctttt
     8401 tgcccatgct gccgcggccc tgctgtgcgg cctgctcggc gcggcaagct gggccttcgt
     8461 cgccagcgag ctggacgccg gccgcgagct ggcctacggc ctgccggtgt ggacggtcca
     8521 ggctgcaatg cccctcggct tcctgctcct gggcatgcgc ctgggcgccc gctgcgcgcc
     8581 ggccggcctc gcgcgccccc tggtgggcgc ctcgctggca ggccttggct tcctgctggc
     8641 ccgccacttc gacggcagca ccctgagctt cgcccccttc gcggtcgccc tgctggccgc
     8701 cctgctggcc ggcgccccca tcttcgcggt gctgggcggc ctcgccctgg gcctgttctg
     8761 gtccgaaggc cagcccctcg cctcggtgcc cctcagccac taccagatca ccgtcaaccc
     8821 gtccctgccg gccctgcccc tgttcaccct ggccggcctg gtgttcgccc gcagcggtgc
     8881 ggccggacgc ctcggcaccc tgttcaccgc ctgcttcggc ggcggcgcca ccggcagcgc
     8941 ggtggccgcg gcggtgctgt gctcctgctt caccgccttc accggcggca gcggcgtcac
     9001 catcctcgcc ctcggcggcc tgctcctgcc cctgctgcgc gacgcgggcc tgcccgaacg
     9061 gcgcggcatc agcctggtca ccagcgccag cgccctgggc gtgctgctcg ccccgtcggt
     9121 gccgctcatc atggtcgcca tcatcgcccg ggtgcccatc aacaccatgt tcctggccgg
     9181 cctgctgccg gccctggtga tggtcctgtg cctcttgctg gtgggcggct acctgcgctc
     9241 cacgccccgc gccggggcgc ccgtcgccgc cccgtccgca ggggtcccgc ctggcgtggg
     9301 cctgcgcgcg gccatctggg cggcccgctg ggagatcctt gccccggtgg tggccatcgg
     9361 cgccctcgcc agcggcatcg ccacgcccac cgagagcgct gccctcaccg ccgcctacgc
     9421 cctgttcacc caggccgtgc tgcaccggga ggtggacgcc accctgctgc gccgctgcct
     9481 ggccgactgc gcccaggtca tcggcggcgt gatgctgatc ctcggcatgg ccctgggcct
     9541 gaccaactac ctggtggatg cgggcatccc ggatgccgcc gtggactggg tgcaggccac
     9601 cctgcccaac aagttcgcct tcctggtggc cctcaacctg ttcctcttcg tggccggcgc
     9661 cctgatggag atctacgccg cgatcgtcgt gctggtgccc ctgctgctgc cggtggcggt
     9721 cagttatgac atcgacccgg tgcacttcgg cgtgatcttc ctggccgtca tggagatggg
     9781 cttcctgtgc ccgccggcag ggatgaacat cttcttcgcc tccgccatgt tcgcccgccc
     9841 catccgcgag gtagccgtgt cggtgctgcc ggccctgctg gcaatcttcc tcggcgccct
     9901 gatcatcgcc tgggtgccct ggctggccac cgggctgccg gcgctgttcg ccctgcactg
     9961 acccacacgc ggcggcaccc gctcaacgcg gacagaatcg atatcccacg ccggattcag
    10021 tgaggaggtg gcgcggccgc gacgggtcgt cctcgatctt cttgcgcagc tgggccatat
    10081 ggacgcgcac gtagtggacg tcctccgcgt gccccggccc ccacaccgtc agcgccagat
    10141 ggcggtgcgt cagcaccgtg tccgggtggg aggcgaggca ggcgagcagg cggaactcga
    10201 tgggcgtcag gtgcaccggc accccgtccc gctcgatcag ccggcgcccg aagtccgccc
    10261 ggaggcgccc gaagctcatc acgccgggcg tgccgtcgcg gggcgccgca cggcgcaact
    10321 gggcccgcac ccgagccagc agctcggccg caccgaacgg cttgacgagg tagtcgtcgg
    10381 cccccgcatc cagcgccgcc accttctccg actcctgggt gcgcgccgac aggacgatga
    10441 cggggatcgc cgaccaggtc cgcagttcgc ggatgaaggc cttgccgtcg ccgtcgggca
    10501 ggcccagatc gagcaccacc agatccgcct ggcgcgtgcc cgcctcgatc agcccgcgct
    10561 tcacggagtc cgcctcgaag gcctgcaggt cggcgctctc gagtgccatg cagacgaagc
    10621 gccggatctt cggttcgtcc tcgatcacca ccacgaccgg ccggggatca gtgcccatgg
    10681 ggcgcgtcct ccgcaagccc gcccatgccc ggcaccgccg gcggctgccc ggcgggcagg
    10741 ctgaacagga agcgggcgcc cccgtccgcc cggttctcgg cccggatggt gccgccgtgg
    10801 gccgcgacga tggcgcgaca gatcgccagc cccagcccca cgccgggggt cgcgctctcc
    10861 gactggccgc gttcgaactt cttgaagatg gcctcctccc gccccgccgg cagccccgga
    10921 ccgtcgtcct cgacccagat cgtcacaaag ccgcccgcgg gcccctccgc accgatgcgg
    10981 accggggtgc ccggcggcgt gtacttgacc gcattctcca ccagattcga cagcacccgc
    11041 tcgatcaaca ccgtatcgaa ctccagcagg ggcagatcgt cgggcagacg gacctccacc
    11101 ggatgacgga agagggccga ctccagcccc ttcagcgcgc tgccgaccac ctcctcgagc
    11161 ggctgccact gcttgttgag ggtcaccgtg cccgcctgca gccgcgccat gtcgagcaga
    11221 ttgctgacca gggcgttcat gcgctgcacc tcgtcgcgca tcgactcggc cagctgcacc
    11281 aaggccggcg caggtgccgg ccgggtgagc agcatcatgt ccaccagccc caccagcgcc
    11341 gacaggggcg tgcgcagatc gtgggagatg gccgacagca gggaattgcg caggcgctcg
    11401 gactccatct gcagtgtcgt ggcctgggct acctggatgt aatggacgcg ctccagggaa
    11461 attgcgatga ggcgcgcgaa ggtctccagc aggcgatgct gctccggcgc gcccatgcgc
    11521 cccggatggc ggggctccag caccagcacg ccgcggatgc gcatgggcgc cttgagggga
    11581 acgtagagca gcggactcgc cggcagcgta tccgtgccgc agccggcctc cgcctcgtgg
    11641 tcgaaggccc actggcagat gcccgcgtcc acgccgctga gcccgcctgg tacggcgagc
    11701 ggcgccagca gacggttggc gtcgtccgcc agcaccagcg tgcagcgcgc cgcaaaggtc
    11761 gtccgtgaga agcgcagcgc gatctctgcg atctgctcgg gcatcagggc cccggagaga
    11821 tcgcgagaca tctcgtagag cgcccggatg cggtattcgc ggcggctcgc cacccgcgcc
    11881 tgcagcttca ggccggcggt aaggttggcc gtgatgaggc cggacgccag catcaccgca
    11941 aaggtgagca cgtactgcac gtcgctcacc gcaaacgaga agcgcggcgg cacgaagaag
    12001 taatcgaagg ccgccacgtt caggaaggac gccaccaccg ccggccccag gccgtagcgc
    12061 acggccgtca ggaccaccgc gagcaggaag accatggcga tgttggccag gtcgaaatgc
    12121 cccagcagca gcgtggcgac cacggtcgcc agggcgccca gggcggcggt catcgcgaag
    12181 gccgtccacg gcgcccgcag ccggtccagc cacgactcgc tggtgggcgc ctccaccgcg
    12241 gcgtcccggc gggcctcgtc gcgggccacc aggatcacgt ccaggtccgg cgcctgccgg
    12301 ccgatctgct cggccatgga gcggtgccat gggcgccagc cgcggggatg atcgcgcccc
    12361 accacgatgc gcgcgagatt gtggtcacgg gcgtaggccg cgatgcgctc cgccgcatcg
    12421 gcccccgcca ggctcgccgt ctcggcgccg gattcctggg ccaggtgcag gttcttgagg
    12481 atgcgctggc ggttccgctc gctcaggtgg cgcaggtccg gcgtctccac gtagatcgca
    12541 tgccagggca cctcgaggcg ccccgccagg cgggcgcagg cccgcaccac gcggtcgccg
    12601 ccttcgccgg ggccgacgca ggccagcagg gattcctgcg tcgcccacac cgtgctcacc
    12661 gccgaatcgc ggcggtagcg caccatctcg ctatcgaccc ggtccgcggt gcggcgcagg
    12721 gccagctccc gcagagcgat caggttgccc ttgcggaaga agttctggat cgcccgctcg
    12781 gcctgctggg gcagatagac cttgccctcc ttgaggcgct ggagcagttc gtccggcggc
    12841 agatccacca gcaccacttc gtcggcggca tcgaaaacat ggtcgggcac cgtctcccag
    12901 acgcgcacgc cggtgatgcc gcccaccacg tcgttgagcg tctccagatg ctggacgttc
    12961 accgtggtgt agacatcgat ccccgcatcg agcagttcct ccacgtcctg ccagcgcttc
    13021 gggtggcgcg agccctccac attggaatgg gccagctcgt ccaccaggat cagggccggc
    13081 tgccgcgcca gcgcgccgtc gatgtcgaac tcggcgaggg tccgcccctt gtaggccacg
    13141 ctccgcggcg gcagcacgtc caggccgtcg agcagtgccg cggtctcgcg tcggccgtgg
    13201 ctctccacca cgcccaccac cacgtcgacg ccctgggccc gctgctggtg agcggcgcac
    13261 agcatcgcgt aggtcttgcc gacgcccgcc gacgcgccga agaagatctt caggcggccg
    13321 cgggcggcgc gcccctccgc ctcacgcaga cggtctagga tccgctgcgg atcccggcgc
    13381 gggtcatcca ttggtgcgcg tcgcgatgat gcccggccgc agcccggcca tgccgaccgc
    13441 cctcaacggg cgcacggaaa ctcgctggac agggcctcgg agacctgctg ggccgcgctg
    13501 cgggacgtac ggggcggcgg atccttgggc agatgacggg ccacggcctg gaacagatgc
    13561 tcctccgtca ggtccgcggg caggcaccac tgcgtccccg aaccggcgtc ggccacaccc
    13621 agcacatagc ccttcacgaa caaggccgtg ccgaccggca cccggctgcg cagcacgctc
    13681 tcgagttcct gcaggtcagc gtcgctgcgg aagtaaccgg cggcctggac tgcgcccccc
    13741 cagcccagca cccccaaaca gaccagcgct ccgacccacc ggcccaggtc cccgcctcgc
    13801 gctgctgtgg cggcgcgtcc ggccatgccg aacgcctctc tgatcccatt catcgtgtca
    13861 cctcccctcg acgggaaaac gttcatccag tgcccggttc agggccagca catggacccg
    13921 cggttcaccg aacagccccc actggcgccc ctcggtgtgg gccgcgacca gcgactccag
    13981 cgccgccggg gcgatgccgc ggagccgcgc gatgcgcccg atctggtagg ccgcggcggc
    14041 cgggctgatg tgcggatcga ggccgctgcc cgacgcctgc accagatctg ccggcaccgg
    14101 ggccggattg cccgggtcgg ccgcgcgcag acgctgcacg cgcgcctgga cggcctcggc
    14161 cagcgccgga ttgagcggcc ccagattcga accggacgac gcggccccat tgtacggctg
    14221 gggcgccgtg gccgagggtc gcccccagaa atgccgtgga tcctcgaaga cctgaccgat
    14281 caggcgggag ccggccacgg cgccgtctgc caggatcaga ctgccggccg cctgcgcggg
    14341 gaagacccac cccgccaccg cggtgacgac agccggatac gccagccccg tgagaaggct
    14401 caacagcccg cacagcatca gggcaggacg cacgatggtg ttcatgtccg gatctcctca
    14461 ggccaggccg ctggcggcca gagccatgtc gatgagcttg atgccgacaa agggcgccag
    14521 caggcccccg agcccgtaga tcgtcagatt gcggcgcagc agcagccccg cgccctgggg
    14581 ccggtagcgc acgccgcgca gggccagcgg aatcagcacg atgatgatca gcgcgttgaa
    14641 gatcaccgcc gacaggatgg ccgaagccgg gctggcgagg gccatgacgt cgagcgcctc
    14701 cagctgcggg taggtgctca cgaaggccgc cggcacgacg gcgaagtact tggccacgtc
    14761 gttggcgatg ctgaaggtgg tcagcgcgcc gcgggtcatg agcatctgct tgcccgtctc
    14821 caccacctcg atcagcttgg tcggattgga atccaggtcc accatgttgc ccgcctcctt
    14881 ggcggcctgg gtacccgtgt tcatggccac cgcgacatcc gcctgggcca gagccggcgc
    14941 atcgttggtg ccgtcgcccg tcatcgccac caggcgccct tccgcctgat gctggcggat
    15001 cagggccagc ttggcctccg gcgtcgcctc ggcgagaaag tcatccaccc cggcctccgc
    15061 cgcgatggcg gccgccgtga gccggttgtc gccggtgatc atcaccgtct tgatgcccat
    15121 ccgccgcagt tcgccgaaac gctcgcggat gccgcccttg acgatgtcct tgagctccac
    15181 cacgcccagc acgcgggcat cctccgccac caccagcggc gtgctgccgc gccgggcggt
    15241 ctcttcgacg atggccgtca cggacgccgg aaagtggccg ccgagtgcct ccacgtggcg
    15301 ccgcagcgcc tcgccggcgc ccttgcggat gcgtgtgccg cccaggtcca cgccgctcat
    15361 gcgggtctgg gcggagaaat gcacgaactg cgcccccagg gcctgcaggt catgctcccg
    15421 cagattgaag cgctgcttgg cgagcaccac gatgctgcgc ccctcgggcg tctcgtcggc
    15481 cagggaggcc agctgggccg cccgggccag cgcctgctcc gtgacgcctt ccgccgggat
    15541 gaacgccgcc gcctgacggt tgcccagggt gatcgtgccg gtcttgtcga ggagcagcac
    15601 gtccacatcg cccgccgcct ccaccgcccg gccggaggta gcgatcacgt tggcctgcat
    15661 catgcggctc atgcccgcca ccccgatggc cgagagcagc ccggcgatgg tggtggggat
    15721 gaggcacacc agcagcgcca ccagcaccgt cagggtcacg ggggcgccgg cgcccgccat
    15781 ctgcacgctg aacaccgaaa agggcagcag ggtcaccgtc accaccagaa agaccagggt
    15841 cagggccacc agcagaatgg tcagggcgat ctcgttgggg gtcttctggc gccgggcgcc
    15901 ttccaccatc gcgatcatgc ggtccacgaa ggtctccccc ggattcaccg tcacccgcag
    15961 caccagccag tccgacagca ggcgcgtgcc ccccgtcagc gccgagaaat ccccgccggc
    16021 ctcgcggatc accggcgcgg actccccggt gatggccgac tcgtccaccg aggcgacccc
    16081 ctcgatcacc tccccatccg ccggcaccgt ctcgccggcc tccaccagac acacgtcgcc
    16141 acgccgcagc gcggtggccg acacctgctg ccacggcgcc ccgtagcggg gcgccgtcag
    16201 ccgcttggcc ggcgtgtcct gcttcagccc ccgcaaggca gccgcctgag ccttgctgcg
    16261 cccctccgcc agggcctccg cgaaattggc gaagagcacc gtgaaccaca gccacagggc
    16321 caccgtgaag atgaatccgg ccggggcctc gccgtccccc gccagggcct ggatgcccag
    16381 caccgtggtc agcaggctgc cgccatagac gacgaacatc accggattgc gccactgcac
    16441 ggccggattg agcttgcgga aggcatccac cagcgccggc cggagcagat ccgggtcgaa
    16501 catcgagagc gtcttgcgcg tcatggtcgg cctcctttca gtgcgccgcg tacagggcaa
    16561 ggtgttcgac gaccggcccg agggccagcg ccggcacata gttgagggcc ccgacgagca
    16621 ccaccgtacc gatcagcagc accacgaaga gcggcccatg ggtcggcatg gtcccggcgc
    16681 cgggggccag ccgcttcttc gcggccagat gccccgccag ggccagcacg ggcacgatca
    16741 caccgaaacg tcccagcccc atcaccacac ccagcacgct gttgtagaac ggcgtgttcg
    16801 ccgacaggcc ggcgaaggcg ctgccattgt tgttggccgc cgaagtgaag gcgtacagga
    16861 tctccgagaa gccatgggcg cccggattgc ccacgcccgc gccccccgcc ggcaccagca
    16921 ccgccaccgc ggtgccggcg agcaccagca ccggggtgac gaggatggcg atggacacca
    16981 tcttcatgtc gtgcacctcg atcttcttgc ccaggtactc gggtgtgcgc ccgatcatca
    17041 ggccggcgat gaacaccgcc agcaccgcaa acaccagcat gccgtagagc ccgctgccca
    17101 cgccgccgaa caccacctcg cccagcatca tgttccacag ggtcaccagg ccacccagcg
    17161 gcgtgaacga gtcgtgcatg ccgttcaccg cgccgcacga ggccagggtc gtgatcaccg
    17221 cgaacagggc cgagtcgtcg ctgccgaagc gcgtctcctt gccctccagg ttgcccgccg
    17281 cgccctcgat cccgagggac gccagcagcg gatgaccctg ggcctcggcg tgcgcgccgg
    17341 ccaccgccat ggcgacgaag agcagggtca tggtggccag gatcgcgcgt ccctggcgcg
    17401 cgtccccgac catccgtccg aagctcacgc acagggccgc cgggatcagg aagatcagca
    17461 gcatctccac gaaattgctc aggggcgtcg ggttttcaaa ggggtgggcc gagttggcgt
    17521 tgtagtagcc gccgccgttg gtccccagca tcttgatcgc ctcctgggac gccaccggcc
    17581 ccagggacag cacctgggtc tgcgtccggg ccggctccgt gaggacacgg ccggacgcat
    17641 cgatcagggg cttgcccgtc gcatcgaggc gtggcgcgtc atagtgcgtg agctccaggg
    17701 tggtcgccag ccgatcgccg cccaggtcct ggaccacgcc ctgccccacc aggaacaccg
    17761 ccaccacgcc cgacagggga agcagcacgt acaacaccat ccgggtcagg tccacccagg
    17821 cattgccgac ggtcgcggcc gaatgccgcg caaagccccg gatcagggcc agcgccacgg
    17881 ccattccggt ggcggccgac agaaagttct gcacgctcag gcccagcatc tgggtcagcg
    17941 gactcatcgt cgactcgcct gcatacccct gccagttggt attggtggca aagctgatcg
    18001 ccgtgttgaa ggccgaatcc ggactgaccg ccgccaggcc ctggggattc agcggcagcc
    18061 cgtcctgcag gcgctgcagc gcatacaccg ccagcgcccc gaccaggttg aacgccagga
    18121 tggcgagcgc gtaggtccgc cagcccatct cctgctcggc gcggacgccg cacaggcggt
    18181 agatcagcgc ctcgatcggg cgccccggac gcagcgcccg ccccccctgc ccttccgcca
    18241 cggcggcgat gtagcgcccc agcggcacgg ccgcggccag gagcaccagc aggtacagcc
    18301 ccagcaggac ccacgcgtgg ctgctcatga gaaatcctcc ggacgcagca gggccaccac
    18361 cagatagacc agcagcccgg cggccagcac cgccgacacc gtcagcatcg cgctcatgac
    18421 cgccgctcca ggcgatcgca gaccaccacc aggccgaaca tcagggccca cagcaccgcg
    18481 agcccaccgc cgtacatgac atccatctca ccgctccctg cccactgaac acgcccccat
    18541 ggtagggcgc cgtgtttcaa gaagctgcaa agatgcgggg ccgcggtatc aagaaatcat
    18601 caagatcgcc ctcccggaag gctgcgacac gcccccgaac ctattccacc accgccccca
    18661 gccgcgccca gcgcaccccc tccggcccgg acgaccacca cacctggcgc gcccggcccg
    18721 ccacgtcggc catgggcacg gggccgaagc ggcgcgaatc ggtggtggcg tcccggttat
    18781 cacccagcac gaagacctgc cccggcggca ccgtcagcac cacatccggc ggcgcctcct
    18841 ccggggcgcg ggtggagccg gtccctccgc gcagcacctg ccagcggcgc ccgtcgaggg
    18901 tctccgtcac cgcaccggac atcaccgggt cggtgctcag ggccacgcca ttcacccaca
    18961 ccgccgcgcc ccgcgtctcc acccggtcgc ccggcaggcc gacgatgcgc ttgatgtaca
    19021 gcctggtgcg gtcattgggg taggtgaaca cggccacgtc gccgcggcgg atctgctgct
    19081 tgcagccggg gcagttgtag cgcttgtcgg cgaacaggat gtcgccccgc agcacgctgg
    19141 gctccatgct cgcggagggg atcgagaagg attccatctg gcggctgcgc accgtgtcca
    19201 ccagcagggg cagggccaga ccgttgcaca ccaggaaggc caccgcgtag gccgcgccgg
    19261 tctgccaggg gcgcggcagg tagtccggcc gcgcccgcgc gccgcgccag gcatccacca
    19321 cggcgtagcc ccaggtgccc agcgccgcga ccacgctcac ccccagggcc ggcatcgtcc
    19381 aggccggcgg caggtagagc cccaccaggg ccatgcccgg cacgcagaga aaggcaaacg
    19441 cgagaaaaag ccacacggcc ttgtccagct cgccgttgta gaactgcccg tagcccggca
    19501 ggaccaggga catcagcgcg gcgcgcaggg gctgacgggg cgacacatcg aacatgatcg
    19561 gttctcctga tgattcagcc ggcagtcgtc accgaccaca cggcaaaggc gagactcaac
    19621 agctgggcga cgccgaacag cccggcgccc acggccgtca gccaggccgc cgcccgcgca
    19681 aacggccgcc cacggccagc cggcgcaccc gtcggtgcgc gggacggcgg gatcggctgc
    19741 gggacggccg gcagcgccgc catcacccgc gccgcaaaat ccgcgggcgg cgccgggaag
    19801 tcctcccgca gcagcgcctc cacggcggga tcgagcacgg catcggccgg ccggtcgaag
    19861 ggatcagtac gcatcggaag gctccagcaa acggcccaga cgctggcgcg cccgggtcag
    19921 acgggtcttg gcggcagcga cggaacagcc ctcgatgcgc gcgatgtcgg ccatggccag
    19981 ctcctccaca taggccaggg cgaggaggct gcggtccgcc agggagagtt gccgcagggc
    20041 ggcgtgcaga cgctggcgct gctgcgccag tgccagcacc tcgggcggct gggggcgcgg
    20101 acaggcgggc tccggcgcat cctccgccgc ggggcgctcg cggacatggt gactgcgccc
    20161 cagttcgttg atcgccaggt ggcgggcaat cgtgaacagc caggtggaca aggcccccat
    20221 ctccggccgg taacggtcca gctgctgcca ggcgcgcacg aaggcgtcct gggccagatc
    20281 ctcggcgagg gccggcgcgc accccatgcg gccgagaaag ccgaagaggg gccgctggta
    20341 gcgcaccacc agcgcggcaa aggcctggcg gtcgccggaa cggaggcggg caagcagatc
    20401 aggcgtgtcc atgggtgagt ccggggtgca tgccgcttat accggaccca cggaaaaagg
    20461 tgacaggccg aatgtgcggc cggcacgccg atacaccccg gccccccggg gcgcctgtcc
    20521 gtctccggac gatccgcgca cgcattccgg cgcaggcatc cgcgcccggc ggaattgcgt
    20581 aaacccgatg aaggcccccg ccggcccgga ccttcggccg acgcgggcgc gccgtccccc
    20641 gcccgccgac cgtgaatatg tcacaccgtg tgtcgtaaac cgcgccggag gccgatgcgc
    20701 aacgcccacg ggacagcatc ctgcaaggct ggcctgctaa cgtatataaa aagttataga
    20761 ggttcctgca acatgctcac tcaaccgatc cgctccaagc aggccgtggc cgccgagcgc
    20821 gaatgggccg gccaggcgcg gcggtcctcc acgcccgacc agcacgccgc cccgccgacg
    20881 cccagcgccg gccggggaaa gctgtggctc ctgctcctcg ccgccgccct catcgcgccc
    20941 aagctcctgc actacttcta agcgcggcaa cggccgcccg tccgccgggc ggccagacgc
    21001 ggccgcggaa cgacacctat ttcagatcga agcggtccag gtccatcacc ttgccccagg
    21061 ccgccgcaaa gtcgcccgca aacctcgcgg ccgcatcggc ctgggcgtac acctcggcaa
    21121 tcgcccgcag ctgtgaattg gagccgaaga ccagatccac ccgcgtcgcc gtccagcgga
    21181 gcgcgccgct gtggcggtcg cggccggtaa aagtctcccc cgtctccgcg gtgggtgccc
    21241 agacggtgcc catgtccagc agattcacaa agaagtcgtt gctgagcacc cccggccgcg
    21301 acgtgaacac cccatggggt gcgcccccca cgttggcccc cagcacccgc agaccgccca
    21361 ccagcaccgt catctccggc gcactgaggg tcagcagctg ggccttgtcc accagcaggg
    21421 cctcggccgg cacgcggaag ctctgcgcct gatagttacg gaagccatcc gcctggggct
    21481 ccagcacggc aaaggaatcc acgtcggtct gatccgcgcg cgcatccacg cgccccggcg
    21541 cgaagggcac gctcaccgtg acgccgccgg cctgcgccgc cgcttccacc gctgcattgc
    21601 ccgccagcac gatcagatcc gccagggaca cctggcgccc gtcggtctgc gcggcgttga
    21661 agtcccgctg gatgccttcc agcgtcgcca gcacgcgggc gagctgcggg ggctgattga
    21721 ccgcccagtc cttctggggc gccaggcgga tacgcgcgcc gttggccccg ccgcgcaggt
    21781 ccgaaccgcg gaaggtgctg gccgatgccc acgcggtgct caccagctcg gcgacgccca
    21841 gcccggcctg cagcacccgc gccttcagac cggcaatgtc ggccgcgtcg atcagcgggt
    21901 ggtcccgcgc cggcagcggg tcctgccaga tcaggtcctc cgccggcacc tccgggccca
    21961 ggtaacgggc cctgggcccc atgtcccggt gggtcagctt gaaccaggcc cgcgcaaagg
    22021 cgtcggcaaa ggccgccgga tcctgctgga agcgccgcgc aatgggcgcg tagatcgggt
    22081 cgaagcgcag ggacaggtcc gccgtggtca tcatcggcgg atgcttcttc gccggatcgt
    22141 gggcgtccgg gatcaggtgc tccggcttca cgtcctgcgc cacccactgc tgcgcgccgg
    22201 ccgggctccg ggtcagcgcc cactcgtagc cgaagagcat gtcgaagtag ccgccgtccc
    22261 aggtggtcgg gttcggcttc cacgccccct cgatgccgct ggtggtggtg tcgccgcccg
    22321 tgccgcggcc gaaggcattg atccagccca ggccctgggc ttccaggggc gcgccttccg
    22381 gttccggccc cacccatttc ggatcaccgg ccccgtgggc cttgccgaag gtgtgaccgc
    22441 ccgccaccag ggccacggtc tcctcgtcat tcatggccat gcgcgcaaag gtctcacgca
    22501 catcgcggcc cgaggccacc gggtccggct ggccgtccgg gccttccggg ttcacgtaga
    22561 tcaggcccat ctgcaccgcc gccagcgggt tttccagatc gcgggggccg ctgtagcggc
    22621 tctccggctg gtcgctggtg gccagccagg tcttctcggc gccccagtag atgtcctcct
    22681 ccggcgccca gatgtcggcc cgcccgccgc cgaagccgaa ggtcttgaag cccatggact
    22741 ccagcgccac gttgccggcc aggatcatca ggtctgccca ggagatgcgg ttgccgtact
    22801 tctgcttgat cggccacagc agacgccgcg ccttgtccag gttgccgttg tccggccagc
    22861 tgttgagggg cgcgaagcgc tggttgccgc tgcccgcgcc gccgcggccg tccgccgtgc
    22921 gataggtgcc ggcgctgtgc caggccatgc ggatgaagag cccgccgtag tggccccagt
    22981 ccgccggcca ccagtcctgg gaatccgtca tcagggtgtg caggtcggcc ttcagggccg
    23041 cgtagtccaa ttgccggaag gccgcggcgt aatcgaagtc cgcccccagg ggattcgacg
    23101 ccggcccgtg ctggtgcagg atcgacaggt tcaactggtt cggccaccag tcccggttgc
    23161 tgcgcgcgcc ggtggtggcg cgggtgccgt gggcggcatg gaaggggcat tgggatgcgg
    23221 tgctcatggg ggtctcctcg tgtggtctca cgcctgatgc ggctgccttg aggctagtca
    23281 gccggcgccg ccgaatccac acaaaccttc caatcgtccg catcgcccca ggctatcggc
    23341 ccgcagcatc ccgaccagac gtgtggaaag cccgcttccg cggcggaaag ccgcctgcca
    23401 tcccgcgccg ccgctcatcc ttgcgcgggc ccgcaagtcc ttgagcaacc gcgcccgcca
    23461 ggcctgctgc ccatggcacg gaagttgtat gaacaatcta cggttctgct tacactgccc
    23521 tggtcccgat gccgcccacc gtcgctccac ccgtgccccc gacccgaccg cccgcggcca
    23581 cctctcctgg caaggacgca cgcccccgca cacccacccc ggccgtcggc agcgccctgc
    23641 ccgcccgccg cctcgccctg tgtgcctgcc tggggctggg cgccgccatc gcccacgccg
    23701 gcggcgtgca gaccctcgac gtggtggaag tcaccgccag cgccaccgac ctggtcggcg
    23761 tggcgggcgc cgccagcgaa ggcacggtca cggcaaaaca cctggccaac cgccccctgc
    23821 tgcgccccgc cgaggtgctg gaggccgtcc ccggcctggt catctcccag cacagcggcg
    23881 acggcaaggc caaccagtac tacctgcgcg gcttcaacct ggaccacggc accgacttcg
    23941 ccaccaccct gctgggcatg cccatcaacc tgcccaccca cgcccacggc cagggctatt
    24001 ccgacctcca gttcctgatc cccgaactcg tggacacggt gcgctaccgc aaaggcccct
    24061 acgccgccga cgtgggcgac ttcggctccg ccggttccgc cgccatcgac tacgtccgcc
    24121 gcctgccaca ggccttcgtc gaactcaccg ccgggtccta cggctaccgc cgcggcctcg
    24181 tcgccggctc gcccgcggtg gccgacggcc acctcctggc cgccttcgaa tggaccggcc
    24241 acgacggccc ctgggccgtc ccggaagatc tgcgcaagct caacggcctg ctccgctaca
    24301 gcgacggcac tcgcagcaac ggctggtccg ccaccgccct ggcctactcc gcccgctgga
    24361 acgccaccga ccaggtcccc ttgcgcgccg tgcagtccgg cctcatcggc cgctacggca
    24421 gcctcgaccc cacagatggc ggcaccaccc agcgcaccag cctgtccgtc gaagcctcgc
    24481 gccaggacgc cgatggctgg ctgcgcggca acgcctacct catcgactac cgcctccagc
    24541 tgtggtccaa cttcacctcc tgcaccctcg gctgcgcccc cggccccggc gaccagttcg
    24601 aacaggaaga ccggcgcagg atcggcggct tcaacctcgc ccacacctgg tacctcaaca
    24661 gcattgcccg cggcgccgac ttcaccctgg gcgtgcagag tcgcgtcgac gacatcggcc
    24721 gcgtcggcct ctataccacc accgcccgcc agcgctgggg cagcgtcgcc cgcgacagcg
    24781 tccggcaagg cagcctcggc ctctacggcg aagccaccgt ccagtggctc gaaaaactcc
    24841 gcagcgtcgc cggcctgcgg gccgaccgct accgcttcga cgtcaccgcc gacaccccgg
    24901 ccaattccgg ccaggtggac gccgccctcg ccagccccaa gctggccctc atcctcggtc
    24961 cctgggcccg taccgaggtc tatctcaact tcggccacgg cttccacagc aacgacgccc
    25021 gcggcatcac cctcaccacc cagccggact tccgcgaccc cgccttcggc acgcccgccc
    25081 acgcctccac ccccctggtg cgcacaaagg gcagcgaaat cggcgtgcgc agcgccctcc
    25141 tccccggcct gcacagcagc ctcgccctgt ggcagctcga catcgcctcc gagctcgtct
    25201 tcaccggcga cgccggcacc accgaagccc gcttccccag ccgccgccgc gggatcgaat
    25261 gggccaacga ttggaccccg ctcaccggcc tcatcgtgga cgccgacttc tccgtctccc
    25321 gcgcccgcta caccgccgtg gacgccggcg tgcccggcga ccgcatcccc ggcgccctcg
    25381 aacaggtcgc ctctgtcggc gtcgcctggg atgcgggcgg cccctggtcc ggcggcgccc
    25441 gcctgcgcta cgtcggcccc cgccccctca tcgaggacaa caccgtccgc gcccccggct
    25501 ccaccctcgt caacctgcgc gccagccacc gcatcgaccc gcgcacgcgg atcagcgtag
    25561 acctcctcaa cgccttcaac cgcaaggtca gcgacatcga ctactactac gcctcgcaac
    25621 tgcgcggcga agccgccccg gtggacgacc tccacaccca ccccgccgaa ccgcgcagcc
    25681 tgcgggtgag tctgcgggtc gggttct
//
LOCUS       DAOQPA010000039        25527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_904_length_25527_cov_16.707601, whole genome shotgun sequence.
ACCESSION   DAOQPA010000039 DAOQPA010000000
VERSION     DAOQPA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 25527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25527
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_25527_cov_16.707601"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKX01_07485"
     CDS             <1..468
                     /locus_tag="PKX01_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ76005.1"
                     /translation="QGRTVDFKNTVIVMTSNLGSQMIQQMSGDDYGVIKLAVMAEVKT
                     YFRPEFINRIDEVVVFHALDEKHIASIARIQLGYLEKRLARLEMHLEVSDEALAELAK
                     AGFDPVFGARPLKRAIQEHIENPLARRILEGQFAAKDTIRVSCDNGRIAFARA"
     gene            667..1143
                     /locus_tag="PKX01_07490"
     CDS             667..1143
                     /locus_tag="PKX01_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ76006.1"
                     /translation="MTQDDDNQNLVLGILFGVIALVIGLVIGLTTYVTGQAQTAKPAV
                     VAEEPEIAEVGEPLVKLYFDSGKAELPANAAEELAKVVAKLHEEPAKLVLISGYHDET
                     GGAAVNAEVSKARALAVKDALATAGVAADKLKLRKPAITLGGADEAEARRVEVRVQ"
     gene            complement(1376..2446)
                     /locus_tag="PKX01_07495"
     CDS             complement(1376..2446)
                     /locus_tag="PKX01_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ76007.1"
                     /translation="MIPVARPRHPASRLFLSARLLAALAALSVVAPPSAAGPLRERLR
                     ERLQADDASGGPLSDLGGGEALSCSDWSRRVARVQALAGAGLRGPAADLSDVAYGAGP
                     REQLDVFVPHDRSHAGGAPLIVMVHGGGWCVGDKAMTGVTTHKVDRWTPHGFVFVSLN
                     YPMVGDGADARAQAAAVARAVAFVQRHAADWGADPARVILMGHSAGAHLVSLVNADAE
                     LRRQAGVGPLLGTVSLDAGAIDVVRQMPVVYPFLKTRYREAFGTSEAGWLAASPYHRL
                     GRDAAPWLGVCSTQRQDDPCGQARAYADRSVRLGLRAEVLPEAMNHGALNADLGLPGP
                     YTQAVERFLAGLDPAVAARLGR"
     gene            complement(2556..2927)
                     /locus_tag="PKX01_07500"
     CDS             complement(2556..2927)
                     /locus_tag="PKX01_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ76008.1"
                     /translation="MPHPNYILLYVTDPSASAAFYTALLACPPVEASPTFALFALDSG
                     LMLGLWARGSVEPAASLGGGGGELALTVASEAAVDACFADWRARGLTILQPPTHMDFG
                     YTCVVADPDHHRLRVFAPARP"
     gene            complement(3045..3731)
                     /locus_tag="PKX01_07505"
     CDS             complement(3045..3731)
                     /locus_tag="PKX01_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HMZ76009.1"
                     /translation="MTRTERLFTLMQILRRHRLPVSGTDLARETGVSLRTLYRDIATL
                     QAQGAHIDGEAGVGYVLRPGFLLPPLMFSAEEIDAIVLGARWVTERADPALAAAARDA
                     LSKVAAVLPAPLRHQLDDVPLLIGPVTDSGRDAHTVAQLRTALRNERKLSIDYRDEHG
                     AASQRVIWPCALGFFERVHVLVAWCERRADFRHFRVDRIERLDLLDAGLPRRRQALLR
                     EWRAREGITG"
     gene            4049..4807
                     /locus_tag="PKX01_07510"
     CDS             4049..4807
                     /locus_tag="PKX01_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76010.1"
                     /translation="MADASALWRQLLQVQEPWTVREARADEFNRCMDVWIGVEAPRSW
                     FGFGRGKPVESGPEVVWRHTGFGGWKIRVHVRPPLGGEMPRQAWAGEAGMPFTRALDR
                     QVLALLHEGVSMAAVCAFLDLPMAELWRYRYALENGQLGSGGQEAPAPAMAGVEPLRN
                     VPRTEAPVAGGVAGSRVPSADDPVWLDLLEGRRQLDVRVLSLQLLLSRTRAQVAVIAD
                     ADVRRMKLRDVHRYFVKNERMLTYELGQLVREAA"
     gene            4804..5787
                     /locus_tag="PKX01_07515"
     CDS             4804..5787
                     /locus_tag="PKX01_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76011.1"
                     /translation="MNAPTNRLMSVDDAATLIRRGAWLMIAGDETLLRRLPAGHWIGG
                     TIPYFMADEGGLASRALLFVTEIPVMAGVPEIRSYDLTSLEHLCEDAPANGFSLIIIP
                     AFSAVHSLYARKAPTFPDMFIKPVAGWVAGTHLDDAGRLSPTVVAGPRLAFDTERAVV
                     LHVPLPDSAYARIDIFNALQQGDGDTLRFPETGFSAERCQVNGVETQFAAYLKARAAD
                     TRLPLVADYGGASVNVSIKGVDETAGRVDFYAPVFDDVDYRLAAPVSFGDPTGAEAEV
                     PPLWSCNCILNYLYHGLEGRRCGSLAGPMTFGEIAYLLLNQTQVVLSIEHV"
     gene            5945..6865
                     /locus_tag="PKX01_07520"
     CDS             5945..6865
                     /locus_tag="PKX01_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ76012.1"
                     /translation="MLRPVLRLLSVLLLLAVTGGARGGTLEAVPADAPEWSEEAPAVA
                     RLLERGYQAERQHLPAQAASHYCAAAREGSTEGQYRLGRLYLIGQGLARDNQLAVNLL
                     AVAAERGHERARSLLGGLPAGDRLPDCLTRGGAPEAAVAVVDEVVPQDIVDRYVRALP
                     ADKRRHAQLIQKLAPRFSVDARLALAIVRTESNFDAAARSPKNAVGLMQLIPDTAERF
                     GVRDALDPEQNIRGGLAYLRTLLAQFKGDVALTAAAYNAGEGAVTRHGGVPPYAETQE
                     YVRRILNFYRAAHHAPQGTRVAVAGGVARS"
     gene            6963..7397
                     /locus_tag="PKX01_07525"
     CDS             6963..7397
                     /locus_tag="PKX01_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HMZ76013.1"
                     /translation="MVVLACLALLIVTTVIHYEVLRGLSLLLPTLTIRPRARLLVVIF
                     STFFAHIVEIALYALVVYALVQWGGYGTLDESSRFSLDVSLYFSAETYTSLGYGDVVP
                     GGAVRLLAGVEALNGLLLIGWSASYTYIAMERFWRDSNGQGG"
     gene            7535..8773
                     /locus_tag="PKX01_07530"
     CDS             7535..8773
                     /locus_tag="PKX01_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ76014.1"
                     /translation="MTQTDLRHVIQALSEALDLVGVDDVAHGKRVGLMAAECARELGL
                     TDAEVRFCFDLGMLHDIGVSSTRTHGKLVGHFDWDGAQDHCETGFALLGRFPPLQPLA
                     LPVRYHHTRWEVLTTIGLPDDVARQANLIYLVDRVDALAASWQARGELLLHTGRIRDE
                     IAGHAGHYFDPRLVDAFLRASARESFWLMQEPRAIQAFLRTRRFDGPAVPASVQELHQ
                     LAGIFACIVDAKSPFTAAHSIGVAGVARLLAAALDMDAAACERIEIAGLLHDLGKLRV
                     PDEILDKPGRLDEAERAVMRTHSFETFQILRDIAGFEDIARWAAWHHEEPNGRGYPFH
                     LQGEQLPVEARILRVADIFQAMIQDRPYRAGLPPPEVAAFMNDLAGQGRVDASIVAVL
                     LPRLDEAVDIGRGLRAACAA"
     gene            complement(8770..9270)
                     /locus_tag="PKX01_07535"
     CDS             complement(8770..9270)
                     /locus_tag="PKX01_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ76015.1"
                     /translation="MSDKTLLIVYHSMTDATRQLAVAVAHGAACEPASVAVRLLRAPD
                     ASPDDVLAADGYVFATPENLASMAGMMKDFFDRCYYPALDRVNGRPYASLVCAGSDGS
                     GAVRQIARIATGWRLKEIAPPLVVCTHAQTLEAILAPKVVGVDALARGEELGAAFATG
                     LAIGAF"
     gene            9430..10149
                     /locus_tag="PKX01_07540"
     CDS             9430..10149
                     /locus_tag="PKX01_07540"
                     /inference="COORDINATES: protein motif:HMM:NF037310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76016.1"
                     /translation="MKLTRLPRPRGEPALAVVSHDLSLCQPVEGIARSLDAALADWAT
                     VAPQLAEVGHALEFGAARHAWPFSEQMAQAWPVAGIERMCAPPDGDAAAADLTLVVWT
                     GALPRRATPDAIRSALRLLTFGVGVPGQGGTLAPVAVSPDELEGVWTADGRLQVSIRC
                     RPAEGPDRVTGREHPQGCFEVINALGEASRAGPQPPGVWCFGAQAPVVPVRPAGYRLE
                     IVDARGGNLCGRIPGVRTSPS"
     gene            10368..10775
                     /locus_tag="PKX01_07545"
     CDS             10368..10775
                     /locus_tag="PKX01_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ76017.1"
                     /translation="MKITVETTIRAPLAAVWRAYTTPEDIVLWNAASDDWHTTRASVD
                     LRVGGTFSSRMEAKDGSFGFDFAGTYTQVVPERLLEYAFGDRRARVEFSEDATGVRVK
                     ISFDAENEFPAEQQRSGWQAILDNFKRLLEGRQ"
     gene            11068..12324
                     /locus_tag="PKX01_07550"
     CDS             11068..12324
                     /locus_tag="PKX01_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ76018.1"
                     /translation="MSSVEPSSRLPGPLRALAHRNFRLYFFGQAVSILGSWIQQVAMA
                     WLVYRLTGSSALLGVTAFAALIPQLLVGPLAGAWIDRHDKRRLLLGVQVLLVAQALIL
                     AGLTWAEAVGPTMLVILAAGVGVLNSFDTPLRQSLIGAFVARREDLPNALALNAMLFN
                     SGRFVGPPIAGLLLGWTSEAACFALNACSFVALFVAVWRVKDSATPRAAGSMGHIFRE
                     GVRYAWETWLVRSLIVILVALNLTASSYAVLLPVFARDVFGGDARTLGWLWGAAGCGA
                     FGSTVFLATRRSVPGLVKAVCAGTAISAAALLVFGLTTRFGVALAAMVGLGFGISVCN
                     VGINMLLQSIAPDRLRGRVVSFFTSARFGFDALGGLLAGVIAAALGAGPTLVGEGVVL
                     VLFVLHLAGRARRLADQVAEHAASRP"
     gene            complement(12337..13656)
                     /locus_tag="PKX01_07555"
     CDS             complement(12337..13656)
                     /locus_tag="PKX01_07555"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ76019.1"
                     /translation="MAFLSRLMGLFGGRKADPAPAPAAPPPARNPRAAPTPPVPAEPR
                     IFVGWTEMIDAKARIGAYLLAPNGLQAGATVSGPQLLEALERERVARFAERRPVLIPI
                     TPTQWAAADFRRIAAPLTVFLLRELPTHAAARQALLAELRSHNLRVALVAADWTSDDP
                     ACAPDMLLVDLRSAPLARIEAQVQQIRRTHPRMVLAAENLDSWSEHRLCQSLGFAYSL
                     GAFTTTADEPDETRQISGSRLVVLELLNLLRTEAPPAQIVATAKRDPAIVLRLLALAN
                     SPAYGLAKQVSNLEEMMMVLGRDALYRWLAVALFRLGHDDGRDQTLLVVALGRAGFLE
                     SLVLESERATRDELFLVGLLSVIDSLLGQPMDKVLDRMTLPPAVTAALLRRDGPYAPY
                     LFLVTALERCRLDQAAMVAEALKIDTAHLIDCYSQAMEWATDELMRD"
     gene            complement(13807..14244)
                     /locus_tag="PKX01_07560"
     CDS             complement(13807..14244)
                     /locus_tag="PKX01_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76020.1"
                     /translation="MLRILLMKKRYWALLALLVVIALDAWLRMPDARSRQLTAALEAQ
                     ASPEMKAYPYHFHVMKFQGDTAWLSTPRNVDVPAARAIAAMFPEVDTHNPDNPAFIAA
                     QQRLGEVQGQARAIVLSQSGVAQVRWELDRDWLTAHAIDVPTH"
     gene            complement(14613..15446)
                     /locus_tag="PKX01_07565"
     CDS             complement(14613..15446)
                     /locus_tag="PKX01_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76021.1"
                     /translation="MAETPNTIKHVASPLARQTLLDSSLTRPVAGVRPIRLLPWLQVV
                     KIGGRAIMDRGADAILPLVAELRALLPEHRMLILTGAGIRARHLYGVGLDLGLPVGSL
                     APLAASEAGQNGHILAAMLAPDGVSYVEHPTIANQLSIHLAAARAVVGSAFPPYHHHE
                     FPTSRIPPHRADTGAFLIADALGAAGLTIVEDVDGVHDADPNGPDGATARLIRETTSA
                     ELADRAGPLPFDRALVDVMATARHIEKVQVVNGLKPGSLTAALRGEHVGTLIHTGARR
                     A"
     gene            complement(15457..16269)
                     /locus_tag="PKX01_07570"
     CDS             complement(15457..16269)
                     /locus_tag="PKX01_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76022.1"
                     /translation="MTPTTTELEQLLMQRSLTDEQLQAAAEAAADFRILPAATVIKIG
                     GQSVIDRGRAAVYPLVDEIVVARKTHKLLIGTGAGTRARHLYSIAAGLNLPAGLLSQL
                     GASVADQNAAMLGQLMAQHGISAVSGAGLAEVPLYISEVNAVIFSGMPPYTLWMRPAA
                     EGVIPPYRTDAGCFLMAEQFGCKAMIYVKDENGLYTANPKTTKNPTFIPRISVDEMKA
                     KGLHDSILEFPVLDLLKAARHVREVQVINGLVPGNLTRALAGEHVGTIITAR"
     gene            16612..17052
                     /locus_tag="PKX01_07575"
     CDS             16612..17052
                     /locus_tag="PKX01_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HMZ76023.1"
                     /translation="MGTNLGGGLLGPVFALAGWTGCVLLLIAFRRLRAGFRGDVHVRA
                     FALGEDASVPLPARLANRNYMNLLELPVLFYVACLMAMMIGRGTPAMVGLAWLYVALR
                     MAHSLVHVTYNHVMHRFAVFAVSNGVLVLMWVLLGLQVFGRAGA"
     gene            complement(17343..17864)
                     /gene="pal"
                     /locus_tag="PKX01_07580"
     CDS             complement(17343..17864)
                     /gene="pal"
                     /locus_tag="PKX01_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HMZ76024.1"
                     /translation="MKRTLLPVLLASLWLGACSTTPKPTVTAADAASDGVRSAPATAG
                     AVSATDVEARKLAEQREALAKKSVYFAFDDFTVAEKYRDVIRQQADFTLAHGADTIAV
                     EGNADERGSREYNLALGQKRAEAVRKSLVLLGVPDARLEAVSFGAEKPRESCHEEHCW
                     AENRRVDFNHRDK"
     gene            complement(17927..18451)
                     /locus_tag="PKX01_07585"
     CDS             complement(17927..18451)
                     /locus_tag="PKX01_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ76025.1"
                     /translation="MKTAIPSPARLATEPAPDALSRPLTLAVLKQFRLIYGSVRQHFR
                     EVEQLCGISGAKVWLLHEISGTPGIGVSELARRLSIHQSTCSQLVDKLEARGLVTKQR
                     LPSDQRRVGLHVTDTAADLLRRIPGPAEGLLPDALQGLSEDSLHVLHDHLAQLIDQLA
                     RLDEPSADKPLADL"
     gene            18627..19856
                     /locus_tag="PKX01_07590"
     CDS             18627..19856
                     /locus_tag="PKX01_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ76026.1"
                     /translation="MDLHSAPFLLLGILLLGGLVGGEAVRRLFNLPRTTGYVLTGIAA
                     GPSGLGWVAPDVLQSAQFFVYLAIGVILFELGHRLPMPGRGLDRRLLGLSLADINGTF
                     LAVGAVMLALGFGIGGALFAAAVAVSTSPAITIATTSDVGADGPATERLFALVAFNNC
                     AAFVALAVALPILPLEGLTASLAGHAPPVAYVAGSLALGTALAAMACQGARWLGPQPE
                     HQHLLLLGLICIGAGLPLGVQMSPLLTLLSLGVATRWMDVRGDVVGLRISSDARVFLV
                     ITFVLAGAALEIGLIVAHWREVAAFALARIAGRMLALRAAAGPLGVDAREAHWLGIGL
                     LPKSSIALVLLGNAGPLSGDVGERLANVLLGAIALMQLVGPICTQMCSRGLGEARRLK
                     GADPVTWMLARLPRRNG"
     gene            19871..20407
                     /locus_tag="PKX01_07595"
     CDS             19871..20407
                     /locus_tag="PKX01_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76027.1"
                     /translation="MRRRGIAGLCALVLALVGWGARAAGVTEALIRTSDSSWWPLLME
                     LSRQGEALGVREQVSPAEQSVSFELETEAAFETSQATLAEPFARFMDAVAAAVGDHPG
                     AHVTIIGFGDRPATRYNPVLLAERVRAVQRTLVQGGVGMADTQVGVRTGSEFDGDGGL
                     RNKAAAGAVIVVRIGSRR"
     gene            20470..20751
                     /locus_tag="PKX01_07600"
     CDS             20470..20751
                     /locus_tag="PKX01_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ76028.1"
                     /translation="MVKSQLVARLHAKFPDLDEETVKEAVTVILNGMARHLSDGDRVE
                     IRDFGVFALHAREARISRNPRTGEPVQVEAKSVVRFKPGKELKSRVEQG"
     gene            complement(20944..23046)
                     /locus_tag="PKX01_07605"
     CDS             complement(20944..23046)
                     /locus_tag="PKX01_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ76029.1"
                     /translation="MTALGLPPLRQELALLPGPTLPDGQPSWTLHDPARNLFFQLDWP
                     TVAMLQRWEMGPDALLDDIAQATTLHLDASHLQTVLAFLKQNQLLATLPGQAAAMAEQ
                     LARRKGGIGQWLLHNYLFFRIPLIRPDAWLTRLAPRIAGLYTRRFVWLTLLAGLFGLL
                     GVMREWDTYRNTLVEMVSWSGALAYGITLIGVKVLHELGHGFTAKRFGCRIPTMGVAF
                     LVMWPVAYTDTNEVWRLTSRRQRLQVAAAGIVTELAIALWATLLWVLLPDGPVRMGAF
                     LLSSSTWISTVLINASPFMRFDGYFLLADALQIPNLHTRAFALARWDLRERLFHLGAP
                     RPEPFPAARHASLILFAWATWIYRLVLFLGIAFLVYHFFIKAVGILLFLVEITWFVAR
                     PLASELAVWRELWPILRTRPATWRSLLLAASGLALILLPWPARVVQSAVLYPISEQVV
                     YAPRNAQLVARHAAPGQVVGRDAPLFELASPELDHRERQNAARREQLAWQSGASAFDA
                     EQRKDWQILNEQLGISQAESRTVQADAGQYHPRATMDGIVRDTDPDLHPGDWLTPREV
                     LARVTGRGILRAVVYVEDEALGRLSVGDRAVFIADGHDGPDLRMHVVEIDSDATHVLG
                     EAELASLFGGTVSVREKHGVLYPERAVYRVVLQSDAELPPPQHRWRGTLAIACAWEAP
                     GLRYVRQAAAVLWRELGF"
     gene            complement(23055..24416)
                     /locus_tag="PKX01_07610"
     CDS             complement(23055..24416)
                     /locus_tag="PKX01_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ76030.1"
                     /translation="MNPLLVLLELGQRARAAGTLPELGFLLVNDTRQLMPYRQALLWF
                     EGEGVRAVSGLLAPEANTPFIQWTHRLMGHLSSAPVTPGPVHTVHAAQLPDDLAAEWS
                     EWLSPHVYWLRLPADPGRSDARAGGVLFVCEAPWDDTLHALVGEWIATWHHAWNARLR
                     PTPWSALVWRQALKKAWHTDGGHPWWKRTPVRIALLIALLLVWPVRLTILAPGELVPV
                     DPAVIRAPLDGVVAQVHVRPNQAVKAGDLLFSFDEAPIATRLEVARQALTTAEAEYRQ
                     LAQLALSDPRSKAQLATLLGKVAEKQAEADFLAGQSARAKVTAPQDGVALLDDPAELI
                     GKPVQTGERIMRVANPRSREIEAWIPIGDAIPLDDAAPVDLFLSASPLSSVSGHLRYL
                     AHDALPRPDGSYAYRLRATLDDATDHRIGMKGTAKVQGHRVPVLYWVMRRPIASIRQF
                     LAW"
     gene            complement(24437..>25527)
                     /locus_tag="PKX01_07615"
     CDS             complement(24437..>25527)
                     /locus_tag="PKX01_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HMZ76031.1"
                     /translation="DNDTLSLSARQADGSALPGWLSFDAATGSFTGVPPFGRTPVDIV
                     VVARDGHSGEVTAGFHFESNVPPPPLAPEPQAPTVLFVPPPPPRVIPDAPPTAPPSPF
                     LPPGSPVALVNPGFDSPPPVSAPLPAVPVPPERPGSDRAPVLAAPPDALPPGSSPLVS
                     VGPLPDGLPAASTRGGLVVTRPDQGAFDVVPARANTITLSAGTFVETVPGVSVSVSAM
                     LDNGQALPPWISFNPGTGTLLVNPPPGLKRDVTIRIVARDQNGQEAVTFVRVRVGSQR
                     TTSSLTPAADALREVRPQVMPLDDLTRPDGSAALPARPVADVARPAGHTGRDSLASQL
                     QRAGRGPGVSGRLQAIAQAAEAARRDGA"
BASE COUNT         3643 a         8185 c         9672 g         4027 t
ORIGIN      
        1 cagggccgca cggtggactt caagaacacc gtgatcgtga tgaccagcaa cctgggcagc
       61 cagatgatcc agcagatgtc cggtgacgat tacggcgtga tcaagctggc ggtgatggcc
      121 gaggtgaaga cctacttccg gccggagttc atcaaccgca tcgacgaggt ggtggtcttc
      181 cacgccctcg acgagaagca catcgcgtcc atcgcgcgca tccagctcgg ctatctggaa
      241 aagcgtctgg cgcggctcga gatgcatctg gaggtcagcg acgaggccct ggccgagctc
      301 gccaaggccg gcttcgaccc ggtgttcggc gcgcggcccc tcaagcgggc gatccaggag
      361 cacatcgaga acccgctcgc ccggcgcatc ctggaaggcc agttcgctgc caaggacacc
      421 atccgggtgt cctgcgacaa cggccgcatc gccttcgcca gggcctgatg tcccaccgcc
      481 cggcgatggc cggggggtgg cggacgcccg ccgcgtgcgg gcgtccatgc atccggtggc
      541 cggcgggccg cagcgggtga cgtcgccttt tttgtgaatt gcagggggaa atcgcgagat
      601 cgtcagggta aaccgctata ctcgcgggct gaattcattc ccactgactt cccgaaggat
      661 cgtgcaatga cgcaagacga cgacaaccag aatctcgttc tgggcattct tttcggtgtg
      721 atcgccctgg tcatcggtct ggtgatcggt ctgaccacct atgtgaccgg tcaggcccag
      781 actgccaagc ccgccgtggt ggccgaagag cccgaaatcg ccgaagtggg cgagccgctg
      841 gtcaagctct acttcgactc cggcaaggcc gagctgccgg ccaacgccgc cgaagagctg
      901 gccaaggtcg tcgccaagct gcacgaagag cccgccaagc tggtgctgat ctccggctac
      961 cacgacgaaa ccggcggtgc cgcggtgaac gccgaagtct ccaaggcccg tgccctggcc
     1021 gtcaaggacg ccctggcgac cgccggcgtg gccgccgaca agctgaagct gcgcaagccc
     1081 gccatcaccc tcggcggtgc cgacgaggcc gaagcccgtc gcgtggaggt ccgcgtgcag
     1141 taagtgctgc cgcgtcgctc acgcgaacaa ggaggccggg acgtgtcccg gccttttttc
     1201 atgtacacgg ccgttccggg cggccgacct tgcccggcgg ggcgatgcgt ctttcgacgg
     1261 ctaccgctgc cggcgcgccg gcggagggag gctaaccgca ggcacgcggc caaccgctga
     1321 tccacggccc gacccgcagg tgtctgcccg cgccgggctg cctgcgagtg aggactcagc
     1381 gcccgaggcg ggccgccacc gccgggtcga ggccggccag gaaacgttcc accgcctggg
     1441 tgtacggacc gggcaggccc aggtccgcat tgagggcgcc atggttcatg gcctcgggga
     1501 gcacttcggc acggaggccc agacggacgc tcctgtcggc ataggcgcgg gcctgtccgc
     1561 aggggtcgtc ctgacgctgc gtggagcaga cccccagcca tggggcggcg tcgcgcccga
     1621 ggcggtgata gggcgaggcg gccagccacc cggcttcgga cgtgccgaag gcctcccggt
     1681 agcgggtctt caggaaggga taaacgaccg gcatctgccg caccacgtcg atggcccccg
     1741 catccagcga gacggtgccg aggaggggcc cgacgcccgc ctgacgccgc agctcggcat
     1801 ccgcgttgac cagcgacacc agatgggccc cggccgagtg gcccatcaga atcacccgcg
     1861 cgggatcggc gccccagtcg gccgcatgac gctgcacgaa ggccactgca cgggcgacgg
     1921 ctgcggcctg ggcgcgggca tcggcaccgt cgcccaccat ggggtaattc agcgatacga
     1981 agacgaagcc gtgtggtgtc cagcggtcga ccttgtgggt cgtcacgccg gtcatggcct
     2041 tgtcaccgac gcaccagcct ccgccatgga ccatgacgat gaggggcgcg ccgcccgcat
     2101 gtgagcggtc atgcgggaca aagacgtcga gctgctcgcg ggggccggca ccgtaggcca
     2161 cgtcggacag atccgcggcc ggtccgcgca ggccggcgcc ggccagagcc tgcacccgcg
     2221 ccacccggcg ggaccagtcg ctgcagctca gggcttcgcc accgcccaga tcgctcagcg
     2281 gtccgccaga cgcgtcgtct gcctgcaggc gctcccgcag ccgttcacgc agggggccgg
     2341 ctgcggacgg cggggccacg acggacaggg cggccagggc ggccaggagc cgggcgctta
     2401 gaaaaaggcg agacgcggga tgacgggggc gtgcgacggg gatcatgggg cggctccggg
     2461 gattgatggc agcccgctgc gggccggcca cgtcatggag gcggcacgca cgggctttgc
     2521 cagggatgac cggttctccg gtccgcaggt tcccgttacg gacgggccgg tgcgaacacc
     2581 cgcagacgat ggtggtccgg atcggccacg acgcaggtgt agccgaaatc catgtgcgtc
     2641 gggggctgga ggatcgtgag cccgcgcgcc cgccagtcgg caaagcaggc gtccaccgcg
     2701 gcctcgctgg cgacggtgag ggcgagctcg ccgcctccgc cgcccaggga ggccgcgggc
     2761 tcgacgctgc cgcgcgccca caggcccagc atcaggccgg aatcgagggc gaacagggcg
     2821 aaggtgggcg aggcctccac cggcgggcac gccagcagcg ccgtgtagaa ggcggcgctg
     2881 gcgctcgggt cggtcacgta gagcaggatg tagttcgggt gcggcatggc agggttcctc
     2941 ggttcggggt cgaagggccg tccggcggca ggccggcggg tggacccatt ctgcggcggc
     3001 gggctgtcag aaaccggcag cagcgcgcac ggcgcggcgc ggcgtcagcc ggtgatgcct
     3061 tcccgcgcgc gccattcccg cagcagcgcc tgacggcgac gcggcagacc ggcgtcgagc
     3121 aggtccagcc gttcgatgcg gtcaacgcgg aaatggcgga aatcggcgcg ccgctcacac
     3181 caggcgacca ggacgtggac acgttcgaag aagcccaggg cgcagggcca gatgacgcgc
     3241 tggctcgcgg cgccgtgctc gtcgcggtag tcgatcgaca gcttgcgctc gttgcgcagg
     3301 gccgtgcgca gctgcgccac cgtgtgcgca tcacgaccac tgtcggtgac cgggccgatc
     3361 agcaggggca cgtcgtcgag ctggtggcgc agcggggccg gcaggacggc cgcaaccttg
     3421 ctcagggcgt cccgcgccgc ggcggcaagg gccgggtccg cacgttcggt cacccagcgt
     3481 gcgccgagca caatggcgtc gatctcctcc gcgctgaaca tcaggggggg cagcaggaaa
     3541 cccggacgca ggacgtagcc cacgccggcc tcgccatcga tgtgggcgcc ctgggcctgc
     3601 agtgtggcga tgtcccggta cagggtgcgc aggctgaccc ccgtctcgcg ggcgaggtcg
     3661 gtaccgctca cgggcagacg gtggcgacgc aggatctgca tcagggtgaa gagacgttcg
     3721 gtgcgggtca tggcggaggg cttcgggact cgcggcatcc tgccacgctt cggccgtccc
     3781 gtcgcgcagg gtggggccgc cgcgggcatg ttgatcggat ttgcagcagg ttggggacgc
     3841 agggtgttca ccggcgggac atggtgcgac gcggcatcgc gcagcccgac ggtgcaatct
     3901 gcctggcatg ggcgaggtgt cgcgcggagc gcgggcggtg acggcctgcg ggggcgctct
     3961 gccggcaaac ggacggattc tggcacgtcc cttgcggccg gttctccgtt ctggcgcctc
     4021 cgggcgtctt ccttcgagcg aggtggctat ggccgatgcg tccgcactgt ggcggcagtt
     4081 gctgcaggtc caggaaccgt ggaccgtccg ggaggcccgg gcggatgaat tcaatcgctg
     4141 catggatgtg tggatcggcg tcgaggcgcc gcgcagctgg ttcggcttcg gtcggggcaa
     4201 gcccgttgaa tccggtccgg aggtggtctg gcggcatacc ggcttcggtg gctggaagat
     4261 ccgtgtgcac gtgcgcccgc ccctgggcgg cgaaatgccc cgccaggcct gggccggcga
     4321 ggccgggatg cccttcaccc gggcgctgga ccggcaggtg ctcgcgctgc tccatgaagg
     4381 cgtgagcatg gcggcggtgt gcgcctttct cgatctgccc atggcggagc tgtggcgcta
     4441 ccgttatgcg ctggagaacg gtcagctcgg gtcgggcggc caggaggcgc ccgctccggc
     4501 gatggccggc gtcgagccct tgcggaacgt gccgcggacg gaggcgccgg tggccggggg
     4561 tgtcgccggc agccgcgtgc cgtcggcgga cgacccggtc tggctggacc tgctggaagg
     4621 gcgacggcag ctggatgtgc gggtgctgag cctgcagctg ctgctgtccc ggacccgtgc
     4681 ccaggtggcc gtgatcgccg acgccgacgt gcgccgcatg aagctgcggg acgtgcatcg
     4741 ctacttcgtg aagaacgagc gcatgctcac ctatgagctg ggacaactgg tccgggaggc
     4801 cgcatgaacg caccgaccaa tcgcctgatg agtgtggacg acgcggccac gctcatccgc
     4861 cgcggcgcat ggctgatgat tgccggcgac gagaccctgc tgcgccgcct gccggccggc
     4921 cactggatcg gcgggaccat cccctatttc atggccgacg agggcgggct ggccagccgg
     4981 gcgctgctct tcgtcaccga gatcccggtg atggcgggcg tccccgagat ccgcagctac
     5041 gatctcacca gcctggagca tctctgcgag gacgccccgg cgaacggctt cagcctgatc
     5101 atcatcccgg ccttctcggc cgtgcattcc ctctatgcgc gcaaggcgcc caccttcccc
     5161 gacatgttca tcaagccggt ggccggctgg gtcgccggca cccacctgga cgacgccggt
     5221 cgcctcagtc ccaccgtggt ggcggggcca cgtctggcgt tcgatacgga gcgggcggtg
     5281 gtcctgcacg tgccgctgcc cgacagcgcg tacgcgcgca tcgacatctt caatgccctg
     5341 cagcaaggtg acggcgacac cctgcgcttt cccgaaaccg gcttttcggc ggagcgctgc
     5401 caggtcaatg gcgtcgagac ccaattcgcg gcctatctca aggcccgcgc cgccgacacc
     5461 cgcctgcccc tggtggccga ctacggcggg gcctcggtga acgtgtccat caagggcgtg
     5521 gacgagaccg cggggcgcgt ggatttctac gcgccggtct tcgacgacgt ggactatcgc
     5581 ctggccgccc cggtgagctt cggcgacccg accggtgccg aggccgaggt accgcccctg
     5641 tggtcctgca actgcatcct caactacctg taccacggcc tcgaaggacg ccgttgcggt
     5701 tccctcgccg ggcccatgac ctttggcgag atcgcctacc tgctgctcaa ccagacccag
     5761 gtggtgctga gcatcgagca cgtctgaggg cgccgctggc ccggggccgc gccgcccgcc
     5821 gcgtgacgga ttgaccgttc caccgaccgc tcggacggga tgtacggggg aatgtcgaat
     5881 gcttcacgcg ctgacgccgg catgggcggc atgatgcgga cgccccgagt ctccatgcgc
     5941 cccgatgtta cgtcccgtcc ttcgcctgtt gtccgtcctg ctcctgctcg ccgtcaccgg
     6001 tggggctcgc ggcggcacgc tcgaggccgt cccggcggac gcgccggaat ggagcgagga
     6061 ggcgcccgcc gtggcacgcc tgctggagcg tggctaccag gccgaacgtc aacacctgcc
     6121 cgcgcaggcc gccagccact actgcgccgc cgcccgtgag ggcagcacgg aaggccagta
     6181 tcgccttggc cgcctgtacc tgatcggtca ggggcttgcc cgggacaacc agctggccgt
     6241 caacctcctc gccgtggccg ccgaacgcgg gcacgagcgc gcccgcagcc tgctgggcgg
     6301 gctccccgcc ggtgatcgcc tgcccgattg tctgacccgc ggcggggcgc cggaggcggc
     6361 cgtggcggtc gttgacgagg tcgtgcccca ggacattgtg gatcgctacg tgcgcgcgct
     6421 gccggccgac aagcgccgcc acgcccagct gatccagaag ctcgctccgc gcttcagcgt
     6481 ggatgcccgc ctggccctgg ccatcgtccg caccgagtcc aacttcgacg ccgcggcccg
     6541 ttcgccgaag aacgccgtcg gcctgatgca gctcatcccg gacaccgccg aacgctttgg
     6601 cgtgcgcgac gccctcgacc cggaacagaa catccgcggc gggctggcct acctgcgtac
     6661 gctgctggcc cagttcaagg gcgacgtggc cctgaccgcc gcagcctaca acgccggcga
     6721 gggcgccgtg acccgtcacg gcggcgtgcc gccttacgcc gagacccagg aatacgtgcg
     6781 ccgcatcctg aacttctacc gggccgcgca tcatgcgccg cagggcacgc gggtggcggt
     6841 cgctggtggc gtcgcccgga gctgatcgcc cttacgtttg ctttcccctt ggtgcagcgc
     6901 cctgaccgtt cccgatggga taatccgggc ctcagcgaaa gaagggacaa ggggaagcgc
     6961 acatggtcgt actggcctgc ctggcactgc tgatcgtcac cacggtcatc cattatgagg
     7021 tcctgcgcgg gctgagcctg ctgctgccga ccctcacgat ccgcccgcgg gcgcgcctgc
     7081 tggtggtgat cttctcgacc ttctttgccc acatcgtcga gatcgcgctc tatgcgctgg
     7141 tcgtgtatgc cctggtgcag tggggcggct acggcacgct ggacgaatcc agccgattct
     7201 ccctcgacgt ctccctgtac ttttcggcgg aaacctacac gtcccttggc tatggggatg
     7261 tggtgcccgg cggcgcggtg aggctgctgg cgggcgtcga ggcgctcaac gggctgctct
     7321 tgatcggctg gtcggcgtcc tacacctaca tcgcgatgga acgcttctgg cgcgacagca
     7381 acgggcaggg gggctgagcg cagcgttgtc cacgcaggcc atgccgcagc gatcaagatc
     7441 ccgggccaac cgtccgataa ggcatgctgg aggccggccc ggcgctgcgt ccgcggactt
     7501 ccctccctcg aactcccttt agcgaggcgc gcccgtgacc cagaccgatc tccgccacgt
     7561 catccaggcc ctctccgaag ccctcgacct ggtgggcgtg gacgacgtgg cccacggcaa
     7621 gcgcgtgggc ctgatggcgg ccgaatgtgc ccgcgaactg ggcctgaccg acgcggaggt
     7681 ccgcttctgc ttcgatctgg gcatgctcca cgacatcggt gtctcatcca cgcgcaccca
     7741 cggcaagctg gtcggccact tcgactggga cggtgcccag gatcactgcg agaccggctt
     7801 cgccctgctc gggcgattcc cgcccctgca gcctctcgcc ctgccggtgc gctatcacca
     7861 cacccgctgg gaggtgctga cgaccatcgg gctgcccgac gacgtggcgc ggcaggcgaa
     7921 cctgatctac ctggtggatc gggtcgatgc cctcgcggcg tcctggcagg cgcggggcga
     7981 actgctgctg cacacgggca ggattcgcga cgagatcgcc ggccacgccg ggcactactt
     8041 cgatccgcgc ctggtggacg cctttctgcg ggcctccgcg cgggaatcct tctggctgat
     8101 gcaggagccg cgggccatcc aggccttcct tcgcacccgc cgcttcgacg ggccggccgt
     8161 cccggcgtcg gtgcaggagc tgcatcagct ggccgggatc tttgcctgca tcgtcgacgc
     8221 caagagtccc ttcacggccg cccactcgat tggcgtggcc ggcgtggcgc gtttgctggc
     8281 cgcggcgctg gacatggacg ccgcggcctg cgaacggatc gagatcgccg gcctgctcca
     8341 cgacctgggc aagctgcggg tgcccgacga gatcctcgac aagcccggcc ggctggacga
     8401 ggccgagcgg gccgtgatgc gcacccacag tttcgagact ttccagatcc tgcgggacat
     8461 cgccgggttc gaggacatcg cccgctgggc agcctggcac cacgaggagc ccaacggccg
     8521 cggttatccc ttccacctgc agggtgagca gctgcccgtc gaggcgcgca tcctgcgcgt
     8581 ggccgacatc ttccaggcca tgattcagga tcgaccctac cgcgccgggc tgccgccgcc
     8641 cgaggtggcc gccttcatga acgatctggc cggccagggg cgggtggacg cgtccatcgt
     8701 ggccgtgctg ctgccgcgcc tggacgaagc ggtcgacatc ggccgcgggc tgcgggcggc
     8761 gtgcgccgcc tagaaggcgc cgatcgccag cccggtggcg aaggccgcgc ccagctcctc
     8821 gccgcgggca agggcgtcca cccccacgac cttgggcgca aggatggctt ccagcgtctg
     8881 ggcatgggtg cacaccacca ggggcggcgc gatctccttc agccgccagc cggtggcgat
     8941 gcgcgcaatc tggcgcacgg cgccgctgcc gtcgctgccg gcgcacacca ggctggcgta
     9001 aggccggccg ttgacgcggt cgagcgccgg gtaatagcag cggtcgaaga agtccttcat
     9061 catcccggcc atgctggcca ggttttccgg ggtggcgaac acgtagccgt cggccgccag
     9121 cacgtcgtcc gggctggcgt cgggcgcccg cagcaggcgc acggcgacgc tggccggctc
     9181 gcacgcggca ccatgggcga ccgccacggc cagctgccgg gtggcatcgg tcatggagtg
     9241 atagacgatg agcagggtct tgtcggacat gggggcgcgt gagcctcgct cgagggtgga
     9301 tccgcaacgg ccggcacgca tcgcccgccg ccggccgctg cgcgtccggt gcatcatcct
     9361 ccgcggcagg acggcgcccg tcaatcgggc cgtccatggg ccggtacggc ccgccagcga
     9421 cgcgcctcca tgaaactgac ccgactgccc cgcccgcgtg gggaaccggc gctggccgtg
     9481 gtgagccacg atctgagcct gtgccagccc gtggagggta tcgcccgcag tctggacgcg
     9541 gcccttgccg actgggcgac ggtcgcgccg cagctggcgg aggtcggcca cgccctggaa
     9601 ttcggcgcgg cgcgccacgc ctggccgttt tccgagcaga tggcacaggc ctggccggtg
     9661 gccggtatcg aaaggatgtg cgcgccgcca gacggtgacg ccgctgcggc ggacctgacg
     9721 ctggtcgtgt ggaccggggc gctgccccgc cgggcgacgc ccgacgcgat ccgttcggcc
     9781 ctgcggctgc ttaccttcgg cgtgggtgtc cctggccagg gcggcacgct ggcgcccgtg
     9841 gcagtctccc cggatgaact ggagggcgtg tggacggccg acgggcgctt gcaggtgtcg
     9901 atccggtgcc ggccggcaga ggggccggac cgtgtgacag ggcgcgagca cccgcagggg
     9961 tgtttcgagg tgatcaatgc cctgggcgaa gcaagccgtg cggggccgca gccgcccggc
    10021 gtgtggtgtt tcggtgcgca ggccccggtg gtgccggtgc ggcccgccgg gtaccggctg
    10081 gagatcgtgg atgcccgtgg gggaaatctg tgcgggagga tcccgggcgt gcggacatcg
    10141 ccgtcctgac ggcccaccgg atgctccggg gcccgcgggc cggccgtacg gatgaggtct
    10201 ccgcagccgg cacggaacgg ggcgtatcgt cgaggcgttc ctgtccatgc ggcgcgggac
    10261 ttccgctttt ccgcccggcg cgcgtaagct cacatctccc caggatgccg gtgggggcgc
    10321 ctccgcgcat tcgatcccat ggcacccatg acccatcagg agagactatg aaaataacgg
    10381 tcgagacgac gatccgggcg cccctcgcag cggtgtggcg ggcctatacg acgcccgagg
    10441 acatcgtcct gtggaacgcc gcctccgacg attggcacac cacgcgcgcc agcgtggatc
    10501 ttcgggtggg cggcaccttc tcgtcgcgca tggaggcgaa ggacggcagc ttcggattcg
    10561 attttgccgg cacctatacc caggtcgtgc ccgagcgtct gctcgaatac gcctttgggg
    10621 atcgccgggc gcgggtcgag ttctccgagg acgccaccgg ggtccgcgtg aagatctcct
    10681 tcgacgcgga gaacgagttt cctgccgagc agcagcggag cggctggcag gccatcctcg
    10741 acaacttcaa gcgtctgctg gaaggtcggc agtaaagggc agactgcgtc ccggtcgtgc
    10801 cgggatgcag tcctctccat tggcgatgtg cggatcttgt ccggccggag caaaggaccg
    10861 caggggcgat tcgggcggtg gggcgacggc ccccgcgtga agccgtcgct gcccgcaggc
    10921 gacacggccg cgaggatgtg gcggatcctc gcggaccgtg acgtcaaggt cctccgtggt
    10981 cgtgccggcc gactgcgact caggcacaat cgcgcacttt cgtgcgtcgt ccgttccggc
    11041 gacgtgcggt gcctcgcgtt gtcctccatg tcatccgtcg aaccctcatc ccgtctgccc
    11101 ggtcccctgc gggcgctggc ccatcgcaat ttccgcctgt atttcttcgg ccaggcggtg
    11161 tccatcctgg ggtcgtggat ccagcaggtg gcgatggcct ggctggtgta tcggctcact
    11221 ggctcgtcgg cgctgctggg ggtcacggcc tttgctgcgc tgattcccca gttgctggtc
    11281 ggcccgctgg cgggggcgtg gatcgaccgc cacgacaagc gccgcctgct gctgggcgtg
    11341 caggtgctgc tggtggccca ggcgctgatc ctggccgggc tgacctgggc ggaggcggtg
    11401 gggccaacga tgctggtgat cctggcggcc ggggtggggg tgctcaacag cttcgacacg
    11461 cccctgcgcc agtccctgat cggggccttt gtggcgcggc gggaggatct gcccaatgcg
    11521 ctggccctca acgcgatgct gttcaactcc ggccgcttcg tcgggccgcc catcgccggt
    11581 ctgctgctgg ggtggacctc ggaggcggcg tgcttcgcgc tcaacgcctg ctccttcgtg
    11641 gccctcttcg tggcggtgtg gcgggtgaag gacagcgcca cgccgcgggc ggccggctcc
    11701 atgggtcaca ttttccggga aggggtgcgc tatgcctggg agacctggct ggtgcgcagc
    11761 ctgatcgtga tcctcgtcgc cctcaacctg acggcttcgt cctacgcggt gctgctgccg
    11821 gtgtttgccc gcgacgtgtt cggcggcgac gcccgcaccc tgggctggct gtggggggcg
    11881 gcggggtgtg gggccttcgg atcgacggtc tttctggcca cgcggcgctc ggtgccgggg
    11941 ctggtgaagg cggtgtgcgc agggaccgcg atcagcgcgg cggcgctgct ggtcttcggg
    12001 ctgacaacgc gcttcggggt ggcgctggcg gcgatggtgg gcctgggatt cgggatctcg
    12061 gtgtgcaacg tgggcatcaa catgctgctg cagagcatcg cgccagaccg gctgcggggc
    12121 cgggtggtgt ccttcttcac gtcggcacgc ttcggtttcg acgccctggg cggcttgctg
    12181 gcgggggtga tcgccgcggc gctcggggcc gggccgacgc tggtgggaga gggcgtggtg
    12241 ctggtgctgt tcgtactgca tctggccggc cgtgcccggc gcctggcgga tcaggtggcg
    12301 gaacatgctg cgtcccgccc ctgaggcggg ggatcatcag tcgcgcatca gctcgtcggt
    12361 ggcccattcc atggcctgac tgtagcagtc gatgaggtgg gcggtgtcga tcttgagggc
    12421 ctcggccacc atcgcggcct gatcgaggcg gcaacgttcc agggcggtga cgaggaacag
    12481 atagggcgcg tagggcccgt cccggcgcag cagggcggcc gtcacggcgg ggggcagggt
    12541 catgcggtcc agcaccttgt ccatgggctg accgagcagg ctgtcgatga ccgacagcag
    12601 gcccaccagg aagagctcgt cgcgggtggc gcgctccgat tccagcacca gggattccag
    12661 gaagccggcc cggcccaggg ccaccacgag cagggtctgg tcccgtccgt cgtcgtggcc
    12721 gaggcggaac agggcgacgg ccagccagcg gtagagggcg tcgcgaccca gcaccatcat
    12781 catctcttcc aggttgctga cctgcttggc cagcccgtag gcgggcgaat tggccagggc
    12841 caggaggcgc agcacgatgg ccggatcgcg cttggcggtg gcgacgatct gagccggcgg
    12901 ggcttcggtg cgcagcaggt tgaggagctc cagcaccacc aggcggctgc cggagatctg
    12961 ccgcgtctca tccggctcgt cggcagtggt ggtgaaggca cccaggctgt aggcgaaacc
    13021 cagggactgg cacaggcggt gctcggacca ggagtccagg ttctccgccg ccagcaccat
    13081 gcgggggtgg gtgcggcgga tctgctgcac ctgggcctcg atgcgcgcca ggggggccga
    13141 gcgtaaatcc accaggagca tgtccggtgc gcaggcagga tcgtcggacg tccagtccgc
    13201 cgccaccagg gccacgcgca ggttatggct gcgcagctcc gccagcaggg cctgccgcgc
    13261 cgccgcgtgg gtgggcaact cgcgcagcag gaagacggtg aggggggcgg caatgcgccg
    13321 gaagtccgca gccgcccact gggtcggcgt gatcgggatg agcaccggcc ggcgctcggc
    13381 aaagcgcgcg acgcgctccc gctccagggc ctccagcaac tgcgggccgg agaccgtcgc
    13441 accggcctgc agcccgttgg gcgcgagcag gtaggcgccg atgcgcgcct tggcgtcgat
    13501 catctcggtc cagccgacga agatgcgcgg ctcggcgggc accggcggtg tcggtgcggc
    13561 acgcgggttg cgggcgggtg gcggggccgc gggcgcaggc gccgggtcgg ccttgcgccc
    13621 cccaaaaagg cccatcagtc gggaaagaaa tgccacggaa ccatccttcg gtcgatacgg
    13681 gagcgtccgg acggcggcgg gaggtcgcag gtggggcgac agcccttcag gccggcggag
    13741 gaggactcct ttatcgaccg gattgaccgg ttctgaatgg tggcggggcc gccccgggga
    13801 tggggctcag tgcgtcggta cgtcgatggc gtgggcggtc agccagtccc ggtccagttc
    13861 ccagcggacc tgggcaacgc cggactggga caggacgatc gcccgcgcct gaccctgcac
    13921 ctcgcccagg cgctgctggg cggcgatgaa ggccgggttg tccgggttgt gggtgtcgac
    13981 ctcgggaaac atcgccgcga tggccctggc ggccgggacg tccacgttgc gcggcgtact
    14041 cagccaggcc gtgtcgccct ggaacttcat cacgtggaag tgataggggt aggccttcat
    14101 ctccgggctg gcctgcgctt cgagtgcagc agtgagctgg cgggagcgcg cgtcgggcat
    14161 gcgcagccag gcatcgaggg cgatgacgac gagcagggca aggagggccc agtagcgttt
    14221 tttcatgagg aggatgcgga gcatccgatg gtgcgtcgga taggaattgg tatattagca
    14281 aatgttaatt gattgcgggc atcggtccgg cgaggggatg cccgttgtgt gggcgcgatc
    14341 cgggttaaag gcccatcgcg gcgtccgtcg ggcggccgcg ggcgcctggg cgtgcgctcg
    14401 gcattcaatg agagcgatgg cgcgacggcg ggggtgcgct ccggcgtgtt gcgtgggtgc
    14461 tcctctgggc gcagcagttc gatctggacg ggtgtgtggg agacggggcc gtggggcgac
    14521 gcactcacga gaacattgcg agcgggccgg gtggttcggc ccatggcgtc agtgaggaat
    14581 tttccgggtc tagacgaccc ggggtgtggg cgtcaggccc ggcgggcgcc agtatggatg
    14641 agcgtgccca cgtgttcgcc ccgcagggcc gcggtgaggc tgcccggctt gagaccgttc
    14701 accacctgca ccttttcgat gtggcgggcg gtggccatca cgtccaccag ggcccggtcg
    14761 aagggcagcg ggccggcccg gtcggccagt tcggcggacg ttgtttcgcg gatcagccgg
    14821 gctgtggcgc cgtcggggcc gttggggtcg gcgtcatgga caccgtccac gtcctcgacg
    14881 atggtcaggc cggcggcgcc gagggcgtcg gcgatcagga aggcgccggt atccgcccgg
    14941 tgcgggggga tgcgcgaggt ggggaattca tggtggtgat acggggggaa ggcgctgccc
    15001 accacggcgc gggcggcggc caggtggatg gagagctggt tggcgatggt gggatgctcc
    15061 acgtaggaga ccccgtccgg ggccagcatg gcggccagga tgtggccgtt ctggcccgcc
    15121 tcgctggcgg cgaggggcgc cagggagccc accggcaggc ccaggtccag gccgacgccg
    15181 tacagatgcc gggcgcggat gccggcgccg gtcaggatca gcatgcgatg ttcgggcagc
    15241 agcgcgcgga gctcggcgac gaggggcagg atggcgtcgg cgccccggtc catgatggcg
    15301 cggccgccga tcttgacgac ctgcagccag ggcagcaggc ggatggggcg cacgccggcc
    15361 acggggcggg tcaggctgct gtcgaggagg gtctggcgcg cgaggggcga ggccacgtgc
    15421 ttgatggtgt tgggggtttc ggccatggcg gattcctcag cgtgcagtga tgatcgtgcc
    15481 cacgtgttcg ccagccagcg cgcgcgtgag attgccgggg acgaggccgt tgatgacctg
    15541 cacctcgcgg acgtgacggg cggccttgag caggtccagc accgggaatt ccaggatcga
    15601 atcgtgaagg cccttggcct tcatctcgtc gacggagatc ctggggatga aggtggggtt
    15661 cttcgtggtc ttggggttgg cggtgtagag gccgttctcg tccttcacgt agatcatcgc
    15721 cttgcagccg aactgttcag ccatcaggaa acagccggcg tcggtgcggt agggcgggat
    15781 gacgccctcg gcggcgggac gcatccacag ggtgtagggc ggcatgccgc tgaagatcac
    15841 ggcgttgacc tcggagatgt acaggggcac ctcggcgagt ccggcgccac tgacggcgga
    15901 gatgccgtgc tgggccatga gctggcccag catcgcggcg ttctggtcgg ccaccgatgc
    15961 gcccagctgc gagagcaggc cggcgggcag gttgaggccg gctgcgatgg aatacaggtg
    16021 gcgggcacgt gtgccggcgc cagtgccgat gagcagcttg tgggtcttgc gggcgacgac
    16081 gatctcgtcc accagcggat agacggccgc gcggccgcgg tcgatgacgc tctggccgcc
    16141 gatcttgatc accgttgcgg ccggcaggat ccggaaatcg gcggctgctt ctgccgccgc
    16201 ctgaagctgc tcgtcggtca gcgagcgttg catgaggagc tgttcgagct ccgtcgtcgt
    16261 gggggtcatg gggatccttt cgttgttgtg aagcgccggc gtggcggggt gcgatcggcg
    16321 gtggggggcg gaccgccagg ccccgttcca ggacggatgc gcccgcccaa gatgggcagg
    16381 tcaccgggtg gacggtagga tggagcgttg tatagaggca accgtatgcg tgaatagcgt
    16441 gatcgggcgg cagcgtgcag cgcgtcctgg tgacacgcgg gtctgaccgc gcctgcatgg
    16501 acgcgggccg tcgtcggaca cgcggggcat cgtgtgcacc gccgtgtgcc gtggcggtcg
    16561 ttcagcccgg cgccgggatg cgctcgggtc gatgtatgag acgggggagc gatggggacg
    16621 aatctgggtg gggggctgct cgggccggtc tttgcgctgg cggggtggac gggctgcgtg
    16681 ctgctgttga tcgctttccg gcgtctgcgt gcgggcttcc gtggcgacgt gcatgtgcgc
    16741 gcctttgcgc tcggcgagga cgccagcgtg ccccttcctg cgcgtctggc caatcgcaac
    16801 tacatgaatc tgctggagtt gccggtgctg ttctacgtgg cctgcctgat ggccatgatg
    16861 atcggccgcg ggacgccggc gatggtcggg ctggcctggc tctatgtggc cctgcggatg
    16921 gcgcacagtt tggttcacgt gacctacaac cacgtgatgc accgcttcgc ggtctttgcg
    16981 gtcagcaatg gggtgctggt gctgatgtgg gtactgctgg ggcttcaggt gttcggcagg
    17041 gctggcgcct gagcccgggg gcattcaggc ggggcggcgg tgtggccgcg gccttcccgg
    17101 ggggaggcct gccctggcag cgtcgcaccg gtgcccggaa tcgcgctgga cgatgacggg
    17161 tcgcctgact gacggaaaat cccagggcgg ggcggccatg cggagccggg ccaggtccgt
    17221 ctcgtcgggc agactggcgc cccagggggg gttggcgtcc cggcctttcc gctgtcgata
    17281 cgcttcccgc ggccgatgtg ccgcgcgcgg gaagcctgtc aagcgggcgc gatacggctg
    17341 gctcacttgt cccgatggtt gaaatccacc cggcggtttt cggcccagca gtgttcttcg
    17401 tgacagcttt cgcggggctt ttcggcgccg aagctgacgg cttcgaggcg ggcgtcgggc
    17461 acgcccagca ggacgaggga cttgcgcacg gcttcggcgc gcttctggcc gagggcgagg
    17521 ttgtattcac ggctgccgcg ttcgtcggca ttgccttcca cggcgatggt gtcagcgcca
    17581 tgggcgaggg tgaagtcggc ctgctggcgg atcacgtcgc gatacttctc ggccacggtg
    17641 aagtcgtcga aggcgaagta gacgctcttt ttggcgaggg cttcacgctg ttcggcgagc
    17701 ttgcgggctt cgacgtcggt ggcggagacg gcgccggcgg tggccggtgc gctgcgcacg
    17761 ccgtcactgg ccgcgtcggc cgcggtgacg gtgggcttcg gcgtggtgga gcaggcgccc
    17821 agccaaaggg aggcaaggag aacggggagg agggtgcgct tcatggaggt gctttctcga
    17881 gggacggaca aaaaagggat gtcgggacgg gacctgcgat tgcggatcac aggtcggcca
    17941 gtggcttgtc ggcggaaggt tcgtcgagcc gggcgagctg gtcgatgagc tgtgccaggt
    18001 gatcgtgcag gacgtgcagg ctgtcttcgc tgagtccctg tagggcgtct ggcagcaggc
    18061 cttccgcggg gcccgggatc cgtcggagca ggtcggccgc ggtgtcggtg acgtggagcc
    18121 cgacgcggcg ctgatcgctg ggcaggcgtt gcttggtcac caggccgcgg gcttcgagct
    18181 tgtccacgag ctggctgcag gtggactggt gaatggagag gcggcgggcg agttcggata
    18241 caccgatgcc cggcgtaccg gagatctcgt ggagcagcca caccttcgcg ccggagatgc
    18301 cgcagagttg ctcgacctcc cggaaatgct ggcgcacgga accgtagatc agccggaact
    18361 gtttcaggac agccagggtg aggggccggg acagtgcgtc cggggccggt tctgtcgcca
    18421 gccgtgcggg ggacggaatt gcagttttca ttggtgtcaa ttatatttgt tgcaatgtat
    18481 ttgtgaagcc ctgattgagc ccatccgggc acattgaacg gatgtcgccc aacgcctgcg
    18541 ccgcagcggg gtggagtgcc agtggcccgc atgtcccggg acggggtgtg cgtggccgtg
    18601 ttcagacttt tttgacctcg tacgcggtgg atctacattc tgcgcccttc ctgttgctgg
    18661 gcattctgct gctgggtgga ctcgtgggag gcgaggcggt gcgtcgcctc ttcaatcttc
    18721 cgcggaccac cggttatgta ttgacgggga ttgcggcggg cccgtcgggc ctgggctggg
    18781 tggcgccgga cgtgttgcag tccgcccagt tttttgtgta cctggccatc ggcgtgatcc
    18841 tcttcgagtt ggggcatcgc ctgcccatgc ccgggcgggg gctggacagg cgtctgctgg
    18901 gcctgagtct ggccgacatc aacggaacct tcctggcggt gggggcggtg atgctggctc
    18961 tgggcttcgg cattggcggg gcgctgtttg cggcagcggt ggcggtatcc acgtcgccgg
    19021 cgatcaccat cgccaccacc agcgacgtgg gcgcggacgg gccggcgacg gagcgcctgt
    19081 tcgcgctggt ggccttcaac aactgcgcgg ccttcgtggc attggccgtg gccttgccga
    19141 tcctgccgct ggaagggctg accgcgtcgc tcgcggggca tgcgccgccg gtggcctatg
    19201 tggccggttc gctggccctg gggacggcgc tggcggcgat ggcctgtcag ggggcgcgct
    19261 ggctcggacc gcagccggag caccagcatc tgctgctgct tgggttgatc tgcatcggtg
    19321 ccgggctgcc cctcggggtg cagatgtcgc ccttgctgac cctgctgagt ctgggtgtgg
    19381 cgacgcgctg gatggacgtg cggggcgacg tggtggggct gcgcatctcg tcggacgcgc
    19441 gggtgtttct ggtgatcacc ttcgtgctgg cgggtgcggc gctggagatc ggcctgatcg
    19501 ttgcccactg gcgtgaggtg gcggcattcg ccctggcgcg gatcgccggt cgcatgctgg
    19561 ccctgcgggc ggccgcaggg cctctcgggg tggacgcgcg ggaggcccac tggctgggca
    19621 tcgggctcct gcccaagtcg agcattgcgc tggtgctgct gggcaatgca ggccccttga
    19681 gcggggatgt cggcgagcgc ctggccaatg tgctgctggg cgcgatcgcc ctgatgcaac
    19741 ttgtcgggcc gatctgtact cagatgtgca gtcgcggtct gggcgaggcc cgccgcctga
    19801 aaggcgcgga tccggtgacg tggatgctgg cgcgcctccc gaggcggaac ggatgagggc
    19861 gttggcaggc atgcgacggc gcggaatcgc ggggctgtgc gcgctggtgc tggcgctggt
    19921 cggctggggt gcccgggcgg cgggcgtgac ggaagccttg atccggacat cggattcgtc
    19981 ctggtggccc ttgctgatgg agctgagccg gcagggcgag gcgctgggcg tccgggagca
    20041 ggtgtcgccc gcggagcagt cggtgagctt cgagctcgaa acggaagcgg ccttcgaaac
    20101 gtcccaggcg accctggcgg agcccttcgc ccgcttcatg gacgcggtgg cggcggcggt
    20161 cggcgatcac ccgggtgccc acgtgacgat catcggtttc ggcgatcgcc cggccacgcg
    20221 ctacaacccg gtgctccttg cggagcgggt cagggcggtg cagcggacgc tggttcaagg
    20281 tggcgtgggc atggccgata cacaggtggg ggtccgtaca ggatccgaat ttgatggcga
    20341 tggtggattg cggaacaagg cggccgccgg ggcggtgatc gtcgttcgca tcgggtcccg
    20401 tcggtgagtg tgcaggtgac gggccgaagc cgtcacgaga gagaggtcat gcaggacgat
    20461 aaacggtgga tggtgaagtc gcagctggtg gcgcgcttgc acgccaagtt tccagacctg
    20521 gacgaagaaa cggtgaagga ggcggtcacc gtgatcctca atggcatggc gcgccatctg
    20581 tcggacggtg atcgggtcga gatccgggat ttcggtgtgt tcgcgctcca cgcgcgcgag
    20641 gccaggatca gccgcaaccc ccgtacgggc gagccggtgc aggtggaggc caagtcggtg
    20701 gtgcgcttca agccgggcaa ggagttgaag tcccgcgtcg agcagggatg acgcgcggcg
    20761 gcccgcggcg ccccgcgatc ggccggcgcg accctggctt gcgtggggtg tcgcggtact
    20821 ccacgcccgg gttccgacgt gccaagcctg atcaggccga ccgcgcatct cggctgacgc
    20881 ggggttgaat ccttctgctc cgggaccacg gcctgtgggc gcccggcccc cggaaggcat
    20941 cgctcagaag cccagctccc gccacaggac cgccgccgcc tgacgtacgt agcgcaggcc
    21001 gggcgcctcc catgcgcagg cgatggccag ggttccacgc caccggtgct ggggcggggg
    21061 aagttccgca tcgctctgca gcaccacccg gtagaccgcc cgttcggggt agaggacgcc
    21121 gtgtttttcc cggacgctca cggtgccgcc gaagaggctg gccagttcgg cttcccccag
    21181 gacgtgggtg gcgtccgagt cgatctcgac gacatgcatg cgcaggtcgg ggccgtcgtg
    21241 accgtctgcg atgaacacgg cccggtctcc cacgctcagg cgacccagcg cttcatcttc
    21301 cacatagacc acggcgcgca ggatgccccg accggtcaca cgggccagga cctcccgcgg
    21361 ggtgagccag tcgcccgggt gcaggtcggg atcggtgtcg cgcacgatgc cgtccatcgt
    21421 cgcgcgtggg tggtactggc ccgcgtcggc ctggacggtc cgtgactcgg cctggctgat
    21481 gcccagctgt tcgttcagga tctgccagtc cttgcgctgc tcggcgtcaa aggccgaggc
    21541 ccccgattgc caggcgagct gctcgcgccg ggcggcgttc tggcgctcgc ggtggtccag
    21601 ttcgggggag gccagctcga agaggggtgc gtcacgcccg acgacctggc ccggtgcagc
    21661 gtggcgggca acgagctggg cattgcgagg cgcatagacc acctgttcgc tgatcgggta
    21721 gagcacggcg ctctggacga cgcgggccgg ccagggaagc aggatcaggg ccagtccgga
    21781 cgctgccaga agcaggctgc gccaggtcgc tgggcgcgtg cgcaggatgg gccagagctc
    21841 ccgccagacg gccagttcac tggccagggg tcgggcgaca aaccaggtga tttccaccag
    21901 gaacagcagg atccccaccg ccttgatgaa gaagtggtac acgaggaagg caatgccgag
    21961 aaacagcacg aggcggtaga tccatgtcgc ccaggcgaac aggatcaggc tggcgtggcg
    22021 cgcggcagga aacggctccg gacgcggcgc gcccaggtgg aacaggcgct cgcgcagatc
    22081 ccagcgggcg agcgcaaagg cgcgggtgtg cagattgggg atctgcagcg cgtcagccag
    22141 caggaaatag ccgtcgaagc gcatgaaggg actggcgttg atgagcaccg tggagatcca
    22201 cgtcgaggac gacagcagga atgcgcccat ccgtaccgga ccgtccggca ggagcaccca
    22261 cagcagggtc gcccacaggg cgatggcgag ctccgtgacg atgcccgctg cggccacctg
    22321 caggcgctga cggcgcgagg tgaggcgcca cacctcgttt gtgtcggtgt aggcaacggg
    22381 ccacatcacg agaaaggcga cgcccatggt ggggatgcgg cagccgaacc gtttggcggt
    22441 gaagccgtgt cccagctcgt ggaggacctt gacgccgatc agcgtgatcc cgtaggccag
    22501 cgcgccggac cacgagacca tctccaccag ggtattgcgg taggtgtccc attcgcgcat
    22561 cacgcccagc agcccgaaga ggccggccag cagggtgagc cacacgaagc ggcgcgtgta
    22621 gaggccggcg atgcggggcg ccaggcgggt cagccaggcg tccggtcgaa tcagtggaat
    22681 gcggaaaaac aggtagttgt ggagcagcca ctgtccgatg ccgcccttgc gccgggcaag
    22741 ttgctccgcc atggccgcgg cctggcccgg cagggtcgcc agcaactggt tctgcttgag
    22801 gaaggccagc accgtctgca ggtgcgaggc gtccaggtgc agggtcgtcg cctgggcaat
    22861 gtcgtcgagg agggcgtcgg gccccatctc ccagcgctga agcatggcca ccgtgggcca
    22921 gtcgagctgg aagaacaggt tgcgggcggg gtcgtggagg gtccagctgg gctgaccatc
    22981 cggcagcgtg gggccgggca gtagcgccag ctcctgacgc agcgggggca gaccaagggc
    23041 ggtcatgggc gcgcctacca ggccaggaac tgacggatgc tggcgatggg ccggcgcatc
    23101 acccagtaca gcaccgggac ccgatgtccc tgaaccttgg cggtgccttt catgccaatg
    23161 cggtggtccg tcgcgtcgtc gagggtggcg cgcaggcgat aggcgtaaga gccgtccggc
    23221 cgtggcaggg cgtcgtgggc gaggtagcgg aggtggccgc tgactgacga gagcggtgag
    23281 gcgctcagga agaggtccac cggcgccgcg tcgtcgaggg gaatggcatc gccaatggga
    23341 atccaggctt cgatttcccg ggaccgggga ttggcgacac gcatgatgcg ttcgccggtc
    23401 tgcaccggct tgccgatcag ttcggccgga tcgtcgagga gcgcgacgcc gtcctggggg
    23461 gccgtgacct tcgcgcgggc cgactgtccg gccagaaagt cggcttcggc ctgtttttca
    23521 gcgaccttgc cgagcagggt ggcgagctgc gccttggaac gggggtcgct gagggcaagc
    23581 tgggccagct ggcggtactc cgcctccgcg gtcgtcaggg cctggcgcgc cacctccagg
    23641 cgggtggcga tgggcgcttc atcgaaactg aacaggaggt cgccggcctt caccgcctga
    23701 ttggggcgca cgtggacctg ggccacgacg ccgtcgaggg gcgcccggat caccgccggg
    23761 tccaccggaa ccagttcgcc cggtgcgagg atcgtgaggc ggaccggcca gaccagcagc
    23821 agcgcgatga gcagcgcgat gcggacaggg gtgcgtttcc accaggggtg gccgccgtcc
    23881 gtgtgccagg ccttcttcag cgcctgccgc cagacgagcg ccgaccaggg cgtcggacgc
    23941 aggcgggcgt tccaggcgtg gtgccaggtg gcgatccatt cgccgaccag ggcatggagg
    24001 gtgtcgtccc agggggcctc gcacacgaac aggacgccgc cggcacgggc gtcgctgcgg
    24061 ccgggatccg cggggagcct gagccaatac acgtggggag acagccattc agaccattcc
    24121 gccgccaggt cgtcgggaag ctgcgccgca tgcacggtgt ggaccggacc gggcgtgacc
    24181 ggagccgacg acagatgacc catcaggcgg tgcgtccact ggatgaatgg ggtgtttgcc
    24241 tcgggcgcga gcagcccgga gacggcccgc accccctcgc cctcgaacca caggagggcc
    24301 tggcggtagg gcatgagctg gcgggtgtcg ttgacgagca ggaagccgag ctcggggagc
    24361 gttcccgcgg cgcgggcacg ctgaccgagt tcgagcagga cgaggagtgg attcatgcgg
    24421 ctccgggggc tgtgcctcag gccccgtcac ggcgggcggc ctcggcggcc tgggcgatgg
    24481 cctggaggcg tccggacacg ccggggccgc gaccggcccg ttgcagctgg ctcgcgaggg
    24541 agtcgcggcc cgtgtgcccg gccggacgcg ccacgtcggc aaccggccgt gcgggcagcg
    24601 cggcgcttcc gtcgggacgg gtcaggtcat cgaggggcat gacctgcggg cggacctccc
    24661 gcagcgcgtc ggccgccggg gtcaggctgc tcgttgtgcg ttggctgcct acccgcaccc
    24721 ggacgaaggt gaccgcttcc tggccgttct ggtcgcgggc cacgatgcgg atggtgacat
    24781 cccgcttcag gccgggcggc ggattgacca gcagggtgcc ggtaccgggg ttgaagctga
    24841 tccatggggg cagggcctga ccgttgtcca gcatggcact gacggagacc gacacgcccg
    24901 gtaccgtctc gacgaaggtg cctgccgaaa gcgtgatggt gttggcacgg gccggcacca
    24961 cgtcaaaggc accctggtcg gggcgggtga ccaccagtcc gccgcgcgtt gacgcagccg
    25021 gcagaccgtc cggcaggggc ccgacgctga ccagtggcga ggatccgggc gggagcgcgt
    25081 ccggcggggc tgccagcacg ggcgcccggt cgcttccggg gcgctcgggt ggcacgggga
    25141 ccgcaggaag gggtgccgac accggcggag gcgagtcaaa gccgggattg accagcgcca
    25201 cgggcgagcc gggaggcagg aagggcgagg gcggcgcggt gggcggtgca tccgggatga
    25261 cgcgcggtgg cgggggcgga acaaagagga ccgtcggggc ttgaggctcg ggcgccagcg
    25321 gcgggggcgg gacgttggat tcaaaatgga agccggcggt gacttcgccc gaatggccgt
    25381 cgcgggcaac gaccacgata tcgaccgggg tccggccgaa ggggggtacg cccgtgaaac
    25441 tgccggtggc ggcgtcgaag ctgagccagc ccggcagcgc gctgccatcg gcctggcggg
    25501 cgctcaggct cagcgtgtcg ttgtcgg
//
LOCUS       DAOQPA010000040        24580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_962_length_24580_cov_17.188705, whole genome shotgun sequence.
ACCESSION   DAOQPA010000040 DAOQPA010000000
VERSION     DAOQPA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24580
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_24580_cov_17.188705"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKX01_07620"
     CDS             <1..528
                     /locus_tag="PKX01_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76032.1"
                     /translation="VVATPPAPRASVTLPPPARATSAAPVVVPAAAAAATATAAAAAM
                     APRTAEAPRPDLPRPEVAAPRPEPVRPAVPTAGDAAPLAAAAPATRPSRGVSRAYAMD
                     GATFYQNGRKFRVQGLDVNEPGMTSEHATQRLQRALDSGNLTVDAVESDGAGHTVAVV
                     RVNGRNVADTVRAAN"
     gene            complement(1001..1849)
                     /locus_tag="PKX01_07625"
     CDS             complement(1001..1849)
                     /locus_tag="PKX01_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HMZ76033.1"
                     /translation="MTEAIESAELHGQPVLKLQTPDGAVALVSLFGGQVLSWRPAGGS
                     ERLYLSPRAVFDGRTALRGGVPVCFPQFAACGPLPKHGFARNRPWTLVTQRSGPDFVL
                     VTLRLTDDDATRALWPHAFVAELSVGIGGGRLDLELEVDNPGDASFQFTAALHTYLQV
                     REVEATRIEGLRGLEYRDSADGDALKRETAISVTVEDEVDRIYHNAPATLLVREDQRS
                     LGVHAENFPDVVVWNPWEHKCAALPDMPADGFRRMLCVEAGAIRQPVELAPGASWWGR
                     QTLVAL"
     gene            complement(1869..2465)
                     /locus_tag="PKX01_07630"
     CDS             complement(1869..2465)
                     /locus_tag="PKX01_07630"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HMZ76034.1"
                     /translation="MDAAPPVRCAWCGEDPLYQAYHDREWGVPLHDDRALFELLILEG
                     AQAGLAWITILRKREGYRAAFRNFDPATVAAFDADDAARLLADPGIVRNRLKIRAAIG
                     NARAFLDLQAEFGSFDAWLWRFVDGRPIHNTWASLKDVPARTAVSDALSKALQKRGFR
                     FVGSTICYAFMQSAGLVNDHLTGCFRHAPVHALSSRPD"
     gene            complement(2465..5152)
                     /locus_tag="PKX01_07635"
     CDS             complement(2465..5152)
                     /locus_tag="PKX01_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HMZ76035.1"
                     /translation="MNEQHYLTPLFEPRSVAIIGASEREMSLGNVLVRNMLDAGFKGK
                     LFAVNPKHEEVLGVPCYKSVEDIPQRLDLAVITTKADRVPAIVDACGRAGTKSVVVLP
                     SGFSDAGPRGRMLEKNTVENARRHRIRLLGPNCLGILRPELGLNASFAHGSATKGSIG
                     LISQSGALCAAILDWARPNNVGFSSVVSLGTSVDIDFGEVLEYMVSDARTESIFLYVE
                     GIKDARRFVSALRAAARVKPVLLIKVGRHPAGSQAALMHSGATVGEDAVFDAALRRAG
                     VIRLYNMGQLFAAANALFSHFRPRGNRLAIITNGGGPGVMAADRAADLRIPQATLSNA
                     TITKLNEFLPQNWSHTNPVDIIGDADPERYGKTLETVLADEGVDGVLTILTPQAMARP
                     TEVAQKIIDIERAVDKPIFTCWMGEEQVREGRKLFEAAGIPTFRTTEPAVELFGHISA
                     YYRNQKLLVQTPSSLSSDLNPPSVESARLVIETALSERRKNLNEMESKALLAAFRIPI
                     AQTVVARSATEAMVLAEELGLPVAMKIDSPSITHKADTGGVRLNLNGLAAVRAAYQEI
                     LDDVKRKRPDAVINGVAIEPMVIKPYGRELMVGAFRDPVFGPVITFGEGGAHVEIQKD
                     RAVALPPLNSYLAQDMIKSTRVSTLLGEYRNMPPINMESLEFVLLRISEMVCELPWIR
                     EMDINPLIVDENGAVAVDARIVVDNIAPTADRYDHMAIHPYPSHLITKVSMPEGEITI
                     RPIKPEDAELEVDFVRKLSPETKFLRFMNTMRELPPAMVARLTQIDYDREMAFVATIE
                     ENDAEVEIGVARYAVNPDGESCEFAIVVAEDWQHRGLARRLMGVLIETARNRGLHSMA
                     GIFLSNNDRMLKFVQSLGFVLSNDPEDNTIKHGILLLQG"
     gene            complement(5399..8170)
                     /locus_tag="PKX01_07640"
     CDS             complement(5399..8170)
                     /locus_tag="PKX01_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76036.1"
                     /translation="MENVLADALPFVAGALAGLLAGVGGILAMRSRGGSTPAETVTAD
                     GAGPHLQPPWRDFFELSSDWLWQTDAALTFVDIPGAPPSPSIAPPGIIGRNWEDVVIP
                     QLSPDFATAHRTTLERRGPITVALTLTSPTGERRHLELRGRPLFDSTGCFRGYCGIGR
                     ETSERARLESALERAEERFHAVLTHSADGYWEQDTNLRYTAVTARSGRLADLAAEHLL
                     GSRRWDLPGVSQTDAPWADHIEQIRQIASFSNFVFSRRNAAGDDIWLSESGVPVFDER
                     GLFAGYCGVTRDITAERARLLALQSRETHYRAVFDDAPSALAVIDTDGTWKSANAALG
                     RTLGVAAETLVGRPLADFQPEDDARDGQALRARILAGEPPPAGRETRFCHADGTERRC
                     RETLAWQPATEDRPDAFVAVIEDITDRHATLVALQAAESRFRQLFDQAPEAMLVVQDN
                     RIRLANLACRRLVCPPGMDGLDGLDPLELIHPDDRTMEAERLRHFLDDPTLEALPPLP
                     VRYQRLDGRPLKIEASGIRLRLDGRDALLFVLRDVARWLSAEQVLRESRAMYRDVVES
                     VNEILFQTNAAHRLTFLNRAWRHITGFDTRLSIGQSLLDFVVEDDRQRVEHELMSIRG
                     GYADSARFEFRLRTQKSGPCWVEATVRPLRDSLDQSIGTSGTLDDITDRKNAEQSQRD
                     LNVELETRVRVRTSQLEASNRELEAFSYSVSHDLRAPLRAIDGFAQIVAEDYGPYLDE
                     TGREYLQRIRAASQRMACLIDDLIDLARLTRQTMKREHVNLSQIAEQIVTELRLEDPD
                     RIVDVHIEPNLYAAADRGLIRVALDNLLRNAWKFTGRRHDAQIRFHAEKRDRHLVYCI
                     SDNGAGFDMNFASKLFLPFHRMHGINEFAGTGIGLATVQRVILRHEGTIWAESTPEKG
                     ASFYFTLSH"
     gene            8458..8534
                     /locus_tag="PKX01_07645"
     tRNA            8458..8534
                     /locus_tag="PKX01_07645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8492..8494,aa:Pro,seq:tgg)
     gene            8563..8636
                     /locus_tag="PKX01_07650"
     tRNA            8563..8636
                     /locus_tag="PKX01_07650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8597..8599,aa:Arg,seq:tct)
     gene            8688..8763
                     /locus_tag="PKX01_07655"
     tRNA            8688..8763
                     /locus_tag="PKX01_07655"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8721..8723,aa:His,seq:gtg)
     gene            9138..10187
                     /locus_tag="PKX01_07660"
     CDS             9138..10187
                     /locus_tag="PKX01_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ76037.1"
                     /translation="MASSVWWKKAFRRPPRLNLALQGGGAHGAYTWGVLDALLEAGIG
                     LEGISGTSAGAMNAVALADGWTRHGADGARESLDAFWEAVADSLPPELEWLHSLNPAE
                     DGGLPGPMRMMMGLTQYFSPYSLNPLEVNPLRDVVRARFDFERIRRDCPLKLFIAATR
                     VRTGKVRLFTTGELTEEAVLASACLPTLHHAVEIDGEAYWDGGFTANPAVYPLVYDCV
                     APDILLVLLNPLQIPEAPRSAEDIAARTAELGFSTTFLREMRMIAHARRYVAEHQSWR
                     PLGVLEQRLARLRFHLIEAKELAEAGQGSKLNASRSFLHSLRDLGRARARHWLLGHRK
                     DVGIRESVDLAKLFS"
     gene            complement(10239..10643)
                     /locus_tag="PKX01_07665"
     CDS             complement(10239..10643)
                     /locus_tag="PKX01_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76038.1"
                     /translation="MSRASLHTHPCFDTHPGHVRRPEHAIRWQQGLPVEWQPHVVAPL
                     YFDHYMDYELAAARILGRDEDDKACYCAHSFVLDGRGGRHPTTAGAHYAESVRAWRLR
                     DGRWLTHRIVIGGHGALERGRGFFSLGEGMPR"
     gene            11111..13150
                     /locus_tag="PKX01_07670"
     CDS             11111..13150
                     /locus_tag="PKX01_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76039.1"
                     /translation="MKGKHWQGWMGICLATAGTAVAADGVFQLGTVEVVGSRLESAPP
                     GETAVSQARLTRFNRDTLGEAVTLVPGASLSRNSRNEDIISVRGFDVRQVPLFIDGIP
                     AYVPYDGYVDFGRFSTADVAELRVAKGAASLLYGPNTLGGAINLVTRKPVSALEGDVR
                     AGLGAGNLNTLSANVGSHQGTWYVQAGASYLDRDWFPLSADFAPGSIPTSAANPTRNV
                     LENGGPRENAYQTDSRLSLKLGLTPNASDEYAVGYVSQHGRKGNPPYAGSAPGQVLTL
                     AGGANSRFWQWPYWDLESTYFIANVALGSQYALKTRLYQDNYTNKILAFTNGSYTTQL
                     SNTTNFPSWYDDSTTGLSLELDSFAFTNHELSVAYHLKQDKHVDNGLALSKHYRDVTT
                     SYVVEDAIRLDDAWRLRLGASHDRRDAKEVYYWPTGATSGSNWLAELVRSLGPTAEAY
                     ASVARKTRFPTIKDRYSASLGSGLPNADLKPEHALHLESGVRGRPWSGARGSAAVFVS
                     HIEDLMQRVNILPVTTCSATATACTQLQNIADARHAGVELALDQDLGPAWQVGGQYTY
                     LDRQNRSNPAVPLTDTPRNKLFAYATWKPVARWEVQASADAENGRRVAYGSGNAATYT
                     RLSGYALANLKAVFRPVPAVALEAGASNLFDANYALADGYPMPGRLWFANARYSF"
     gene            13165..14469
                     /locus_tag="PKX01_07675"
     CDS             13165..14469
                     /locus_tag="PKX01_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMZ76040.1"
                     /translation="MELLRSPARRAFLRGAGALAAAAGAGPLQAAPRRDVVVLTAYPD
                     AVVSRFEATFEKAHPEYRLRVVWRMPHDALPYLRQAGQGGVDVYWSASPRTYAQLKAE
                     GAFRRAGIDRSGLPGQIGGTQIDDPDGFYLASEVAGYGFACNPAALRARGLGVPQDWT
                     DLADPAYAGQLVMPDPVEVGFAPVMVDIPLQAYGWDTGWALWSAIAANSRFAGRGGSF
                     VADEVGSGRRAVGLSIDFFVAEAIAGGAPLSFAYPRQGGVNPAHIAITAGSPNPAGAR
                     AFMAFVLSEGGQRLLTHPDIRKLPVRPSVYRGLPPTYHNPFAAGAEGGYRYDNARGRG
                     RAPVVAAAFGQALVRPHARLAVLWARLRRAEAPVWNRVWPLLTAPPLSEAQADDEELQ
                     MAFFTRRDDAAAEARAAATEQAWADQVDARLAQAEQAAGLPA"
     gene            14466..15809
                     /locus_tag="PKX01_07680"
     CDS             14466..15809
                     /locus_tag="PKX01_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HMZ76041.1"
                     /translation="MKVSNGGVAALVLLTLLGAAPRARADAAETADRLFTGREAFSQP
                     LPGLDEAERERFFRGRALFNQSWVVAPARDDRVDGLGPLYNRLACVSCHAHNGRGLPP
                     ERAGERMQAMLVRLSVAGVGPRGGPRPHPAYGDQFNEEGIPGVPGEGRVLLRWQTVRV
                     HGARLRRPVLRFEDLAYGPLGPVLTSPRVGQQVVGMGLIDAVPAATLEALAEAPKPDT
                     VRGRVNRVWNPETGATEVGRFGHKANMPSLRAQIAGAFLGDLGITSPLHPAENCTPVQ
                     TACRRAPSGGAPELSAQQLDEVTYYLAQLAVPPRRGADDPAVRRGEARFAALGCTACH
                     RPMLRTGDSASPALAHQDIAPYTDLLLHDMGPGLADWRPDFAAGGREWRTPPLWGLGL
                     TAAVSERARYLHDGRARSLHEAILWHGGEATVARQRYAALGAAGRAELMAFLGSL"
     gene            complement(15955..16383)
                     /locus_tag="PKX01_07685"
     CDS             complement(15955..16383)
                     /locus_tag="PKX01_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HMZ76042.1"
                     /translation="MKISARNVFGGTVTAVKPGAVTTEVDVTLPGGIVIVATVTRDSA
                     IELGLIPGRPVKAIIKASSVLVLTEGADVRLSARNCLAGTITALTEGPVSTEVAIGLA
                     DGHTVYATITHEGSVALGLKLGAPATAVFKAPSVILGVST"
     gene            16544..17371
                     /locus_tag="PKX01_07690"
     CDS             16544..17371
                     /locus_tag="PKX01_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HMZ76043.1"
                     /translation="MTTRRPIHHKLVGTLTVDTEFGTFLGDTRIRLLEAIERYGSISQ
                     AAKAVPLSYKAAWDAVDAMNNLAEEPLVERSVGGKHGGGTSLTEYGQRVIALYRAVEL
                     EYQQAIDRLAKTLGEPGAGSVRQFQTLLRRMSMRTSARNQFVGTVSGLREGEVSFEVR
                     VRLDDAHEIVAVITRESAENLELAIGLEVHAFVKAPSVIIVTDPETRTSARNHLWGEV
                     TRIHPGPVSSEVTITLPSGRNVTSVVTHDSIDMLALAPGVPACAVFQASAVILAVFD"
     gene            17394..18149
                     /gene="modA"
                     /locus_tag="PKX01_07695"
     CDS             17394..18149
                     /gene="modA"
                     /locus_tag="PKX01_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ76044.1"
                     /translation="MKHTFRHASLFLVLAGAALLARAEEISVAVAANFTAPMQKIAAD
                     FEKATGHKAQLVSGSTGKFYAQIKSGAPFQLLLAADDETPARLIKEGDGVSGSAFTYA
                     IGKLVLWSPKPGFVDDKGEVLKAGRFEHLSVANPKLAPYGLAAMEALKALNLADALQP
                     KVVLAESISQAQQFVTSGNAELGFIAYSQIHKDGRIIDGSYWLVPARLYGPIRQDAVI
                     LAQGKGNAAVEALTAYLKSPAAAAVIRSYGYDR"
     gene            18183..18872
                     /gene="modB"
                     /locus_tag="PKX01_07700"
     CDS             18183..18872
                     /gene="modB"
                     /locus_tag="PKX01_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HMZ76045.1"
                     /translation="MELGPDDLNALLLTAKLATVVTLALLVVGTPIAWWLARTRSRLR
                     GVIGAFVSLPIVLPPTVLGFYLLLLMGPQGLVGQTTRALGLGMLPFSFAGLVVASVFY
                     SLPFVVQPIQNAFLAMGERPLEVAATLRASPWESFFRVALPQARQGFVTAAVLGFAHT
                     VGEFGVVLMIGGNIPGKTQVLSVQIYNHVEALEYGKAHGLAAGLVAFSFLVLLLLQAL
                     GGGRNTTGVGR"
     gene            18869..19945
                     /gene="modC"
                     /locus_tag="PKX01_07705"
     CDS             18869..19945
                     /gene="modC"
                     /locus_tag="PKX01_07705"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HMZ76046.1"
                     /translation="MSALSLVLRLPLADFELHAELALPGRGVTALFGPSGSGKTTLLR
                     CVAGLARGAGRIVVNDEVWQDDARRIFLPTHRRALGYVFQEASLFEHLTVRGNLEFGL
                     RRILPAERKVSWDQALDLLGIGPLLERRSTTLSGGERQRVAIARALLTSPRLLLMDEP
                     LAALDAPRKQEILPYLERLHDELAIPVLYVSHSADEVARLADHLVLLERGRVVASGAT
                     DALLARLDLPLARDEEAAVRLDTMVLDHDEAYALTTLGFAGGTIQVTRKALPVGARAR
                     LKIQARDVSIALRGDNPSSIVNRVPARVVGFGEAAHPAQCLVALEAGRVRLMARITRL
                     SRDQLGLAAGMEVVAQIKSVALLA"
     gene            20066..20539
                     /locus_tag="PKX01_07710"
     CDS             20066..20539
                     /locus_tag="PKX01_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HMZ76047.1"
                     /translation="MDTPRIALALLAASLALPAAAINKCQTPDGRVTYTDGPCAAGST
                     QTGRVEPLPPVDPADRADALARSKQVVDDARALDAKRDALAAERQKSYDAERRAEEEA
                     RLRQQAADEAATANLYPVPVIVPRVRAPVVPVVKPRPRTEDTDRPAVMGNYPVRR"
     gene            complement(20664..21809)
                     /locus_tag="PKX01_07715"
     CDS             complement(20664..21809)
                     /locus_tag="PKX01_07715"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HMZ76048.1"
                     /translation="MCHSFARSRPLTLGVELELQLVSLRDFDLTRAATDLLDGLKNDG
                     RHGDVKLEITESMIEINTRPRADVTAIAQDLNALRGLLVLQCARNNVATCGGGTHPFH
                     RWPERRISPGERFAFLSDRYGYLSKQFTVFGQHIHIGVASGNDAIWLTHALNTFVPYF
                     IALSASSPYLDATDTGFQSSRLTAISAFPLSGQAPDIQDWDDFVRHLELLKACGVAQS
                     IKDIYWDVRPKPEYGTVEVRVCDTPLDVDRATEMAAFAQCLASYLLINRPTLTPALQA
                     VVARYNKFQASRYGFNARLADPVNRRALVLGETLDDLLEALKPHAHDLGCSLQIECLK
                     KVLREGASDADWLRKKLTEYSSLPELVRLSTERFTQASSYTSSASRQ"
     gene            complement(21815..23071)
                     /locus_tag="PKX01_07720"
     CDS             complement(21815..23071)
                     /locus_tag="PKX01_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ76049.1"
                     /translation="MNPLATTWVALQEKLYFLHPLADQPDQLVLSALLLVAGMISGEA
                     LHRLLGLSRIIGYVAAGALAGPGALDWLDARTLSMAEPLADTALGLLLLETGRSLDVK
                     WLRTNRELLPSAIAESSLSLILVFAFSYFAADLSAGWSAATAAITMASAPAVVLLTTQ
                     ENRAQGQVAQRTLLLTAINCALSFVVFAAVLGIVHAEQSGNWLNAVAHPLWVMAGGVG
                     IGMLGARIALLLASKIATGAVVRTFALIATAVFTIGLARTLSVPVFLSLFMMGVSLNL
                     IDRKRVLSYIELPNVHWILAIGLFVITGAMLPLKDLNWLTAQLAFGLIVVRGVAKFIG
                     VTLFGQGDLSMKKRRMVGLGIQPLSATAIFMASEIASIYPEVGSEALLLPLLAAAMME
                     FVGPQLCRYALITAGECPPDVKGDLL"
     gene            23505..24284
                     /locus_tag="PKX01_07725"
     CDS             23505..24284
                     /locus_tag="PKX01_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HMZ76050.1"
                     /translation="MSEDRALRIGLSARLFHPNGDARGLHSKTLQVLEQSIAQWVMSR
                     DVLVVMVPSIVRDGPIHRSNIRLGDYAAYLDGLVLQGGADVSPYAYGERPLCDVWEGD
                     PLRDAYEMELIEAFVSVGKPILGICRGMQLINVAFGGSLYQDIPTQLPDAGSHSDEAY
                     DQHSHAVSFCEGGYFAEWFRGAGGQVVSIHHQAIKSLGRNLVAEAVSPTDGVIEAIRL
                     ASDPFVLGVQWHPEFHRPGSDSLLDCAPLLDAFLTAARRHV"
BASE COUNT         3919 a         7915 c         8495 g         4251 t
ORIGIN      
        1 gtggtggcga cgccgcccgc gccccgcgcc agcgtgaccc tgccgccgcc cgcgcgggcg
       61 acgtctgcgg cgccggttgt cgtgcccgcc gctgcagccg cggcgaccgc tacggccgct
      121 gccgcagcga tggctccgcg gacggccgag gcgccgcgcc ccgacctgcc cagacccgaa
      181 gtcgccgcgc cgcggcccga gccggtgcgt cctgcggtcc cgacggccgg ggacgctgcg
      241 cctctggcgg cggcagcccc cgccacgcgt ccgagtcgcg gcgtgagccg ggcctacgcg
      301 atggatggcg cgacctttta ccagaacggt cgcaagttcc gggtgcaggg actcgatgtg
      361 aacgagcccg gcatgacctc cgaacacgcc acccagcgcc tgcaaagggc cctggactcc
      421 ggcaatctga ccgtggatgc ggtggaaagc gatggcgccg gtcacaccgt ggcggtggtc
      481 cgcgtgaatg gtcgcaacgt ggccgatacg gtgcgcgcgg cgaactgagc tgcacccgat
      541 ggccggtggt tggcgatctg ccagcccgat agggcgcgtc cgccggtcca ggcctgacgc
      601 gtgacagggc gccaggtccc ggcggacggg cgtgtgacag tgcgcgcctt gagtgccgcc
      661 cgcccccgaa cccctgcgcg ggtgaccgcc gccacgtgcc cttcatggcc gccaaggcgg
      721 gaagcgggac gcgctgtgcc gtcacgcgcg aacccggtgg gtggcccggt gcggggcgca
      781 cgtttgccag gctgacgtcc ggcacgcgac acggctgcgg ctggccggag gctgacgccc
      841 ggccctgctc ccgggcgatg tcgcggctgg gtttgtgggg cacacgaccg tcgctgcgga
      901 cccacgcagg cgccggtcgg gcgctccgca ggccgcggcg ggtgggcgtc cgcggacgcc
      961 gccgcgccgt acggaccgcg aacgtgcggg tggctcggcc ttacagggcg acgagggtct
     1021 gccggcccca ccagcttgcg ccgggggcca gttccacggg ctgccggatc gccccggcct
     1081 cgacgcacag catgcgccgg aagccgtcgg ccggcatgtc ggggagcgca gcgcacttgt
     1141 gctcccacgg attccacacc accacgtcgg ggaagttttc ggcgtggacg cccaggctcc
     1201 gctgatcctc gcggaccagc agggtggcgg gggcgttgtg gtagatgcgg tccacctcgt
     1261 cttcgacggt cacgctgatg gcggtctccc gcttgagggc gtcaccgtct gcactgtccc
     1321 ggtactccag tccgcgcaga ccttcgatgc gcgtggcttc cacttcccgc acctgcaggt
     1381 aggtgtgcag ggccgcggtg aactggaagg aggcgtcgcc ggggttgtcc acttccagtt
     1441 ccagatccag gcgaccaccg ccgatgccca cgctcagttc ggcgacgaag gcatgaggcc
     1501 acagggcgcg ggtcgcgtcg tcgtcggtca ggcgcagggt gaccagcacg aagtctggtc
     1561 cggaacgttg cgtcacgagc gtccacggcc ggttgcgggc gaagccgtgc ttgggcagcg
     1621 ggccacaggc ggcgaactgg gggaagcaca cgggtacgcc accgcgcagg gcggtgcggc
     1681 cgtcgaacac ggctcgaggg ctgaggtaca ggcgctccga tccgccggcc ggacgccacg
     1741 acaggacctg tccgccgaac aggctgacga gggcgacggc gccgtcgggg gtctgcagct
     1801 tgaggacggg ctggccgtgg agttcggcgc tttcgatggc ttcggtcatg gcggcggggg
     1861 ctgcggattc agtcgggccg cgatgatagc gcatgcaccg gggcatggcg gaagcatccc
     1921 gtgaggtgat cgttcaccag cccggcggac tgcatgaagg cgtagcagat ggtgctgccg
     1981 acaaagcgga agccgcgttt ctgcagggct ttgctgaggg cgtcggagac ggccgtgcgg
     2041 gccggcacgt ccttcaggct ggcccaggtg ttgtggatcg gtcggccatc gacgaagcgc
     2101 cacagccagg cgtcaaagct gccgaactcg gcctggaggt ccaggaaggc gcgggcattg
     2161 ccgatggcgg cgcggatctt gaggcggttg cgcacgatgc cgggatcggc gaggaggcgg
     2221 gccgcgtcgt cggcgtcaaa ggccgccacg gtggccgggt cgaagttccg gaaggcggcg
     2281 cgatagcctt cccgcttgcg cagaatggtg atccacgcca gcccggcttg ggcgccttcg
     2341 aggatgagga gttcgaagag cgcccggtcg tcatggagcg gcacgcccca ttcccggtcg
     2401 tggtaggcct ggtacagggg atcttccccg caccaggcgc agcggacggg cggggcggcg
     2461 tccatcagcc ctggagcagc aggatgccgt gcttgatggt gttgtcttcc gggtcgttgg
     2521 acagcacgaa gcccaggctc tggacgaact tgagcatgcg gtcgttgttg gacaggaaga
     2581 tgccggccat gctgtgcagg ccacggttgc gcgcggtctc gatgagcacg cccatcaggc
     2641 ggcgggccag accgcggtgc tgccagtcct cggccacgac gatggcgaac tcgcaggact
     2701 cgccgtccgg attcacggcg tagcgcgcca cgccgatctc cacctcggcg tcgttctcct
     2761 cgatggtggc gacgaaggcc atttcccggt cgtagtcgat ctgggtgagg cgggcgacca
     2821 tcgcgggggg cagctcgcgc atggtgttca tgaagcgcag gaacttggtc tcgggcgaca
     2881 gcttgcgcac gaaatccact tccagctcgg cgtcttcggg cttgatcggg cggatggtaa
     2941 tctcgccttc cggcatcgac accttggtga tgaggtgcga cgggtagggg tggatcgcca
     3001 tgtggtcgta acggtcggcg gtgggggcga tgttgtccac cacgatgcgc gcatccacgg
     3061 ccacggcgcc gttctcgtcc acgatcagcg gattgatgtc catctcgcgg atccacggca
     3121 gctcgcagac catttcggag atgcgcagca gcacgaactc cagggattcc atgttgatgg
     3181 gcggcatgtt gcggtattcg cccagcagcg tggacacccg ggtggacttg atcatgtcct
     3241 gggccaggta gctgttcagc ggcggcaggg cgacggcgcg gtccttctgg atctccacgt
     3301 gggcgccgcc ttcaccgaag gtgatgaccg gaccgaacac cgggtcgcgg aaggcgccga
     3361 ccatcagttc gcggccgtag ggtttgatga ccatcggttc gatggcgacg ccgttgatga
     3421 cggcgtccgg gcgtttgcgc ttcacgtcgt ccaggatttc ctggtaggca gcacgcacgg
     3481 cggccagacc gttgaggttg aggcgcacgc cgccggtatc ggccttgtgg gtgatggacg
     3541 gggaatcgat cttcatcgcc acgggcagac cgagctcctc ggcgagcacc atcgcttcgg
     3601 tggcggagcg ggcgacgacc gtctgggcga tgggaatgcg gaaggccgcc agcagggcct
     3661 tggactccat ctcgttgagg ttcttgcggc gctcggacag ggccgtttcg atgaccaggc
     3721 gggcgctttc caccgacggg ggattgaggt cggaggagag ggacgagggc gtctgcacca
     3781 gcagcttctg gttgcggtaa taggccgaga tgtgcccgaa cagttcgacc gccggctcgg
     3841 tggtgcggaa ggtggggatg ccggccgctt cgaagagctt gcgcccttcg cggacctgct
     3901 cttcgcccat ccagcacgtg aagatcggct tgtccaccgc ccgttcgatg tcgatgatct
     3961 tctgcgccac ctcggtgggg cgcgccatcg cctggggcgt gaggatggtc aggacgccat
     4021 ccacaccttc atcggcaagc accgtctcga gggtcttgcc gtaacgttcc gggtctgcgt
     4081 cgccgatgat gtccacaggg ttggtgtggg accagttctg cggcaggaac tcgttgagct
     4141 tggtgatcgt ggcgttggac agggtggcct gggggatgcg caggtcggca gcgcggtcgg
     4201 ccgccatgac acccggcccg ccgccattgg tgatgatggc gaggcggttg ccgcgaggcc
     4261 ggaaatggga gaacagggcg ttggccgcgg cgaagagctg gcccatgttg tacaggcgga
     4321 tgacgcccgc gcggcgcagg gcggcgtcga agaccgcgtc ctcgccgacg gtggcgcccg
     4381 agtgcatcag cgcggcctgg gagccggcgg ggtggcggcc caccttgatc agcagcaccg
     4441 gcttgacgcg ggccgcggcg cgcagggcgc tgacgaagcg gcgggcatcc ttgatgcctt
     4501 ccacgtacag gaagatgctc tcggtgcggg catccgacac catgtattca aggacttcgc
     4561 cgaagtcgat gtccaccgag gtgcccagcg agaccaccga cgagaagccc acgttgttgg
     4621 gccgcgccca gtcgaggatg gctgcgcaca gggcgccgga ctgggagatc aggccgatcg
     4681 agcctttggt ggcgctgccg tgggcaaagc tggcgttgag gcccagctcg gggcgcagga
     4741 tgccgaggca gttggggccg agcaggcgga tgcgatgccg gcgggcgttt tcgacggtgt
     4801 tcttctcgag catgcggccg cgcgggccgg cgtcggagaa gccggaaggc agcaccacca
     4861 cggacttggt cccggcccga ccgcaggcgt cgacgatggc cggcacgcgg tcggccttgg
     4921 tggtgatcac cgccaggtcg agacgctgcg ggatgtcttc gacactcttg taacagggca
     4981 cgcccagcac ttcctcgtgc ttggggttca ctgcgaagag cttgcccttg aagccggcgt
     5041 ccagcatgtt gcgcacgagg acgttgccga gggacatctc ccgttcgctg gcgccgatga
     5101 tggcgacgga gcggggttcg aagagcgggg tgaggtaatg ctgttcattc atgatggatg
     5161 ctcgcgtgag cgcggcgacc ctgccgcgcg gtatgccgtt gatacggttt tctcgtcgtc
     5221 tctgttccgg gttgtcaggg cggattctac gcccgacatg ttgcattgca tcggacgaac
     5281 gtttgaatat cgcagcgcac aaccgccgga catgactttt ttcatgaatt ccggggctga
     5341 ggtgttgcat ggcagcaacg tttccgccag caggttgaca ccgtaaatgt ctacggcgtc
     5401 aatggcttag ggtgaagtag aaactcgcac ccttttcggg ggtggattcg gcccagatgg
     5461 tgccttcgtg gcgcaggatg acgcgttgca cggtggccag accgatgccg gtgccggcga
     5521 actcgttgat gccgtgcatg cggtggaagg gcagaaagag cttcgaggcg aagttcatgt
     5581 cgaagcccgc gccgttgtcc gagatgcagt agaccaggtg gcggtcccgc ttttctgcgt
     5641 ggaagcggat ctgggcgtcg tggcggcgcc cggtgaattt ccacgcgttg cgcagcaggt
     5701 tgtccagcgc gacgcggatc aggccccggt ccgcggcggc atagagattg ggctcgatat
     5761 gcacgtccac gatccggtcc gggtcttcga ggcgcagctc ggtgacgatc tgttcggcga
     5821 tctggctcag attgacgtgc tcccgcttca tggtctggcg ggtcaggcgg gccaggtcga
     5881 tcaggtcgtc gatgaggcag gccatgcgct ggctggcggc gcggatgcgt tgcagatact
     5941 cgcggccggt ctcgtccagg taggggccgt agtcctccgc gacgatctgg gcgaagccgt
     6001 cgatggcccg cagtggcgcg cgcaggtcgt gggagaccga ataggaaaag gcctccagct
     6061 cccggttgga ggcctcgagc tgggaggtgc gcacgcgtac gcgggtttcc agttccacat
     6121 tgaggtcgcg ctgggactgt tcggcgttct tgcggtcggt gatgtcgtcc agggtgccgg
     6181 aggtgccgat gctctggtcc aggctgtcgc gcagggggcg gaccgtggct tcgacccagc
     6241 aggggccgga cttctgggtg cgcaggcgga attcgaagcg ggcgctgtcg gcgtagccgc
     6301 cgcggatgga catgagttcg tgttcgaccc gctggcgatc gtcctcgacg acgaagtcga
     6361 gcaggctctg accgatggac aggcgggtgt cgaagccggt gatgtggcgc caggcccggt
     6421 tgaggaaggt gagccggtgg gcggcgttgg tctggaagag gatctcattg accgactcga
     6481 ccacgtcccg gtacatggca cggctttcgc gcagcacctg ctcagccgac agccagcggg
     6541 ccacgtcccg cagcacgaag agcagggcgt cgcggccgtc caggcgcagg cggatgcccg
     6601 aggcctcgat tttgagcggg cggccgtcga gtcgctggta gcgcaccggg agcggcggca
     6661 gggcctccag cgtcgggtcg tccaggaagt ggcgcaggcg ctcggcttcc atcgtccggt
     6721 cgtccgggtg gatcagctcc agcggatcca ggccgtccag gccgtccatg cccggcggac
     6781 agaccagacg gcggcaggcc aggttggcga ggcggatgcg attgtcctgc acgaccagca
     6841 tggcttccgg cgcctggtca aagagctggc ggaagcgcga ttcggcggcc tgcagggcga
     6901 cgagggtggc gtgacggtcg gtgatgtcct cgatgacggc gacgaaggcg tcgggccgat
     6961 cctcggtggc cggctgccac gccagcgtct cgcgacagcg ccgctcggta ccgtctgcgt
     7021 ggcagaagcg ggtttcgcgt ccggcagggg gcggctcgcc cgccaggatg cgcgcgcgca
     7081 gggcctggcc gtcgcgggca tcgtcttccg gctgaaagtc ggcgagaggg cgtcccacga
     7141 gggtctcggc tgccacgccc aatgtccgtc caagggccgc attggcggac ttccaggtgc
     7201 catccgtgtc gatcaccgcc agggcgctcg gcgcgtcatc gaacacggca cggtagtgcg
     7261 tttcgcgcga ctgcagggcg agcaggcggg cgcgctcggc ggtgatgtcg cgggtcacgc
     7321 cgcaatagcc ggcgaagagg ccgcgctcgt cgaagaccgg cacgccggat tcggacagcc
     7381 agatgtcgtc accggcggca ttgcggcggg aaaagacgaa attcgagaag gaggcgatct
     7441 ggcggatctg ctcgatgtga tcggcccagg gcgcatcggt ctgggacacg ccgggcagat
     7501 cccagcgccg gctgccgagc aggtgttcgg cggccagatc ggcgaggcgg cccgagcggg
     7561 cggtgacggc ggtgtagcgc aggttggtgt cctgctccca gtagccgtcg gcgctgtggg
     7621 tcaggactgc gtggaagcgt tcctcggcgc gttccagggc gctctcgagg cgcgcacgtt
     7681 cgcttgtctc gcggccgatg ccgcaatagc cccggaaaca gccggtggaa tcgaagagcg
     7741 gtcggccgcg cagctccagg tggcggcgct cgccggtcgg gctggtgagg gtgagggcca
     7801 ccgtgatggg accacggcgt tccagggtgg tccggtgggc ggtggcaaag tccggactga
     7861 gctgggggat caccacgtcc tcccagttgc ggccgatgat gccgggcggc gcgatgctgg
     7921 ggctcggtgg tgcgccgggg atgtccacga aggtgagcgc cgcatcggtc tgccacagcc
     7981 agtcggagga cagctcgaag aagtcgcgcc agggcggctg aaggtgtggg ccggcaccgt
     8041 cggccgttac ggtctcggca ggggtggatc cgccgcggct gcgcatcgcg aggatgccgc
     8101 ccaccccggc gagcagtccg gcgagggccc cggcgacgaa gggcagggcg tcggccagga
     8161 cgttctccat gaccgggtcc tgcgccgccc gggcagccag cggcagggcg gtcgccaggc
     8221 aggcgaacgc gaccgggcgt gacgaagcca acgcggtaag gacggggcgg aaaaggttca
     8281 ccagactatt atcgtcggct gcggccggtt tgttccgcac cggaccgggg cataaaagca
     8341 ggagcttagt gcgaccctgc aaaattgtgt gtccatgcgc gtatcccctt cggtattaaa
     8401 tcacaccgcg attcgtgttt gacgagcggg ggacagatcg gtatgattcg cgcctctcgg
     8461 ggtgtagcgc agtccggtag cgcgcttgct ttgggagcaa gatgtcgggg gttcgaatcc
     8521 ctccaccccg accaaggctt tgcccccgtg acccgcggcg atctgcccgt agctcaattg
     8581 gatagagcac cggccttcta agccgggggt tacaggttcg atccctgtcg ggcaggcttc
     8641 tacaatttgg ttccggaatg tcggcgcgag tcggcagtcc ctcagtggcg gcattagctc
     8701 agttggtaga gcactggatt gtgattccag gtgtcaccgg ttcgatcccg gtatgtcgcc
     8761 ccatgcaaca tggcagaaag ccccgagcaa tcggggcttt ctgctttccg gggtgtccgc
     8821 catcggccct gcgtgcccgc ggctgccgga ggccgtcagg gtggaccggg cgtcgagggt
     8881 gggcagcgcc gtccacacgc ctgtggtcct gcagttcacc gtgatctcca ccgtcacgcg
     8941 gacgggtgtc gcgccggcac ccggcgcgcg tccccttggc atgcgacgca tccgttgtcc
     9001 gcccgggttc gcgggcaagg tcgggctgtg cccgggcgct gccggacatc cgcacgagag
     9061 aacttccccc gatcgtcccg ctctataaaa tggtgcggcg cacaatccgt gcggcgactc
     9121 attcggatgg agggcctatg gcgtccagtg tttggtggaa gaaggcattc cggcgtccgc
     9181 cccggctcaa tcttgccttg caggggggcg gggcgcacgg cgcctacacg tggggtgtgc
     9241 tcgatgccct gctggaggcg ggcatcggtc tggaaggcat cagcggcacc agcgccggcg
     9301 cgatgaatgc ggtggcgctg gccgacggct ggacgcgcca cggcgccgac ggcgcccgcg
     9361 aaagtctgga tgcgttctgg gaggcggtgg cggacagcct gccgccggaa ctggaatggc
     9421 tgcacagcct caatccggca gaagatggcg gtctgcccgg accgatgcgg atgatgatgg
     9481 gtctgaccca gtacttctcg ccgtattccc tgaatcccct ggaagtgaat cccctgcggg
     9541 acgtggtccg cgcgcgcttc gacttcgaac gcatccgccg ggactgtcca ttgaagctgt
     9601 tcatcgcggc gacgcgggta cgaacgggca aggtgcgcct gttcaccacg ggcgagctca
     9661 cggaagaggc cgtgctcgcg tcggcctgcc tgccgaccct gcaccatgcc gtggaaatcg
     9721 acggcgaggc gtactgggat gggggattca cggcgaatcc ggcggtctat ccgctggtgt
     9781 acgactgcgt ggcgccggac atcctgctgg tgttgctcaa tccgctgcag attccggaag
     9841 cgccccgttc ggcggaggac atcgccgccc ggacagccga gctgggcttt tccaccacct
     9901 ttctgcgcga gatgcgcatg atcgcccacg cgcgtcgcta cgtcgccgag caccagagct
     9961 ggcggccgct gggggtgctg gagcagcgcc ttgcccgcct gcgctttcac ctgatcgagg
    10021 cgaaggagct ggccgaggcg gggcagggga gcaagctcaa tgcgtcgcgc agcttcctgc
    10081 acagtctgcg tgatctcggg cgtgcccgcg cccggcactg gctgctgggt caccggaagg
    10141 acgtggggat ccgggagagt gtcgatctgg caaaactttt ctcttgaagc gtccccgcgg
    10201 cgagcgctat gccgtccgca gcggacggac catgccgctc agcgcggcat gccctccccg
    10261 aggctgaaga agccccgccc tctttccagc gccccgtggc cgccgatgac aatccggtgg
    10321 gtcagccagc gtccgtcccg caggcgccag gcgcgcaccg attctgcgta gtgggcccca
    10381 gccgtggtgg ggtggcgccc gccccgcccg tcgaggacga agctgtgggc gcagtaacag
    10441 gccttgtcgt cctcgtcgcg gccgaggatg cgggccgcgg ccagctcgta atccatgtag
    10501 tgatcgaaat agaggggcgc caccacgtgg ggctgccatt cgacgggcag gccctgctgc
    10561 cagcggatgg cgtgctcggg tcggcgcacg tggccggggt gggtgtcgaa gcaggggtgg
    10621 gtgtggagac ttgcgcggga catggtcagg tcgctttctg cgttgaactc gggtgggggt
    10681 gcgacggtgg ccggaaaccc gtgtgcgtca tcccgcgagc gtgctccggc tgtgccgggt
    10741 catgagcaat ttgaatgccc ggattcggcg tcgtggaacg tttccgttcc aatgatggat
    10801 atgtcgcgga atatatagcg ttaggcctga gcagcccgcc tgccggactg gcacggagca
    10861 gcgaccccgt tggcggttaa aagtggcgga ttcatccggg ccgtctgaat ccggcgcgcg
    10921 ccgtcgtaaa tccctcatca ctgtcgcttt gttgacgacg ttatgtagca atgtatacat
    10981 ccgttgctgt gaaaggcgct tttctctgca gatggcagat ggcacggtga ttgcacgacg
    11041 atagccaggc gcggttacgt aacgcgttat atccatgtgt atataggttg tcgtgcaact
    11101 cgggggcatc atgaaaggaa agcattggca gggctggatg ggcatctgtc tggcgacggc
    11161 gggaacggcc gtggcggcgg acggcgtctt ccagttgggg acggtcgagg tcgtcggtag
    11221 ccggctcgaa tcggcgccgc cgggagagac cgcggtgagt caggcgcgcc tgacccgctt
    11281 caaccgggac accctgggcg aggccgtgac cctggtgccc ggtgccagcc tgtcccgcaa
    11341 cagccgcaac gaggacatca tctccgtgcg cggctttgac gtgcgtcagg tgcccctctt
    11401 catcgacggc attcccgcct acgtgcccta cgacgggtac gtggatttcg gccgcttcag
    11461 caccgccgac gtggccgagc tgcgcgtggc caagggcgcg gcgtccctgc tgtacggccc
    11521 taacaccctg ggcggtgcca tcaacctggt gacgcgcaag cccgtgtcgg ccctggaagg
    11581 ggacgtgcgc gccgggctcg gcgccggcaa cctgaacacg ctgtcggcga acgtgggcag
    11641 ccaccagggg acctggtatg tgcaggccgg cgcgtcctac ctggatcggg attggtttcc
    11701 cctgtcggcg gattttgcgc cgggttcgat ccccaccagc gccgcgaatc ccacccgcaa
    11761 cgtcctcgag aacggcgggc cgcgggaaaa tgcctaccag accgacagcc gcctgtccct
    11821 caagctgggc ctgaccccca acgccagcga cgagtacgcc gtcggctacg tcagccagca
    11881 cggccgcaaa ggcaatccgc cctacgcggg cagcgcgccg ggccaggtcc tgaccctcgc
    11941 cggcggcgcc aacagccgct tctggcagtg gccctactgg gatttggaga gcacctattt
    12001 catcgccaac gtggccctgg gcagccaata cgcgttgaag acgcggctct accaggacaa
    12061 ctacaccaac aagatcctgg ccttcaccaa cggcagctac acgacgcagc tgtccaacac
    12121 caccaacttc ccgagctggt acgacgattc caccaccggg ctgtcgctgg aactggacag
    12181 cttcgccttc accaaccacg agctgtcggt ggcctaccac ctcaagcagg acaagcacgt
    12241 ggacaatggg ctggccctca gcaagcacta ccgggacgtg accacgtcct acgtggtgga
    12301 ggacgcgatc cgcctggacg acgcatggcg cctgcgcctg ggcgccagcc acgaccggcg
    12361 cgacgcgaag gaggtgtatt actggcccac cggcgccacg tctggcagca actggctggc
    12421 ggagctcgtc cgcagccttg gccccacggc cgaggcctac gccagcgtgg cccgcaagac
    12481 gcgctttccc accatcaagg accgctactc cgcctccctc ggctcgggcc tgcccaatgc
    12541 cgacctcaag ccggagcatg ccctgcatct ggaaagcggc gtgcgtggcc gtccgtggtc
    12601 cggcgcacgg ggcagcgccg ccgtcttcgt cagtcacatc gaagacctga tgcagcgggt
    12661 gaacatcctg ccggtgacca cctgcagcgc caccgccacc gcctgcaccc agctccagaa
    12721 catcgccgac gcccggcatg ccggcgtcga gctggccctc gaccaggatc tcggaccagc
    12781 ctggcaggtg ggcggccaat acacttacct ggaccgccag aaccgcagca atcccgccgt
    12841 gcccctcacg gacacgccgc gcaacaagct cttcgcctac gccacctgga agcctgtcgc
    12901 ccgctgggaa gtccaggcct cggccgacgc ggagaacggg cgcagggtcg cctacggcag
    12961 cggcaacgcg gccacttaca cacgtctgtc cggctacgcc ctggccaacc tcaaggccgt
    13021 cttcaggccg gtgccggcgg tcgccctgga ggcgggtgcc agcaacctgt tcgacgccaa
    13081 ctacgccctg gcggacggct atcccatgcc gggccgcctg tggtttgcca acgcgcgata
    13141 cagcttctga tgccggagat cgccatggaa ctgctccgtt cgcccgcccg ccgggccttt
    13201 ctgcgcggtg ccggcgccct cgccgcggcg gcgggcgccg ggcccctgca ggccgccccg
    13261 cggcgtgatg tggtggtgct caccgcctac ccggacgcgg tggtgtcgcg cttcgaggcc
    13321 accttcgaga aagcccatcc cgagtaccgc ctgcgtgtcg tctggcgcat gccccacgac
    13381 gcgctgccct atctccgcca ggcggggcag ggcggggtgg atgtgtactg gagcgcctcg
    13441 ccgcgcactt acgcccaact caaggcggaa ggcgccttcc ggcgcgccgg catcgaccgc
    13501 agcggcctgc cggggcagat cggtggtacg cagatcgacg atcccgacgg cttctacctg
    13561 gcaagcgaag tcgcgggcta cggcttcgcc tgcaatccgg ccgccttgcg ggcgcggggt
    13621 ctgggcgtgc cccaggactg gaccgacctg gccgatccgg cctacgccgg ccagctggtg
    13681 atgcccgacc ccgtggaggt gggctttgcg ccggtgatgg tggacatccc cctgcaggcc
    13741 tacggctggg atacgggctg ggccctgtgg agcgccattg cggccaacag ccgcttcgcc
    13801 gggcgcggcg gcagctttgt tgccgacgag gtgggcagtg gacggcgcgc cgtcggcctg
    13861 tccatcgact tcttcgtggc tgaagccatc gccggcggcg cgccgctgag ctttgcctat
    13921 ccccgccagg gcggcgtcaa tccggcccac atcgcgatca ccgccggcag cccgaatccg
    13981 gccggcgccc gggccttcat ggccttcgtg ctgtcggagg gcgggcagcg cctcctcacc
    14041 cacccggaca tccgcaagct gcccgtgcgg ccctcggtct atcgcggcct gccgccgacg
    14101 tatcacaacc cctttgccgc aggagccgaa ggtggctatc gctacgacaa cgcgcgaggt
    14161 cgcggacggg cgccggtggt ggctgcggcc ttcgggcagg ccctggtgcg cccacacgcg
    14221 cgtttggcgg tgctgtgggc ccgcctgcgg cgcgccgagg cgcccgtgtg gaaccgggtc
    14281 tggcccctgc tgacagcgcc gccgttgagc gaggcgcagg cggatgacga agagcttcag
    14341 atggcctttt tcacccgacg cgacgatgca gcggccgaag cccgcgccgc tgccaccgag
    14401 caggcctggg ccgaccaggt ggatgcccgt cttgcccagg ccgagcaggc ggcggggctg
    14461 ccggcatgaa ggtctcgaac ggcggcgtag ccgcactggt cctgttgacc ctgctgggcg
    14521 cggcgccccg tgcgcgggcc gacgcagcgg agacggccga ccggctgttt accggccggg
    14581 aggccttctc ccagcccctg ccggggctcg acgaggccga gcgggagcgc ttcttccgcg
    14641 gccgcgccct gttcaaccag agctgggtcg tggcgccggc cagggacgac cgggtggacg
    14701 gtctcgggcc gctctacaac cgcctcgcct gcgtgtcgtg ccacgcccac aacggccgcg
    14761 gcctgccgcc ggagcgggcg ggcgagcgga tgcaggcgat gctggtgcgc ctttcggtgg
    14821 cgggcgtcgg gccgcggggc ggtccgcggc cccatccggc ctacggcgac cagttcaacg
    14881 aggaaggcat ccccggcgtg cccggcgagg ggcgggtgct gctgcgctgg cagacggtcc
    14941 gcgtccatgg cgcccgcctg cgccgaccgg tgctgcgctt cgaggatctg gcgtatggcc
    15001 ccctcggccc ggtgctgaca tccccccgcg tcggccagca ggtggtgggc atgggtctga
    15061 tcgatgcagt gcccgcggcc acgctggagg cgctcgccga ggcgcccaaa ccagacaccg
    15121 tgcgcgggcg ggtgaaccgg gtgtggaatc ccgaaaccgg cgccacggag gtcggtcgct
    15181 tcggccacaa ggccaacatg cccagcctgc gcgcccagat cgccggggcc ttcctcggcg
    15241 acctgggcat cacctcaccg ctgcatcctg ccgaaaactg cacgccggtg cagaccgcct
    15301 gccgtcgggc gccgtccggc ggcgcgccgg agctctcggc ccagcagctc gacgaggtca
    15361 cgtattacct cgcccagctg gctgtgccgc cccgtcgcgg ggcggacgat ccggcggtgc
    15421 gtcgtggcga ggcgcgtttc gcggcgctgg gctgcactgc ctgccaccgg ccgatgctgc
    15481 gcaccggcga ctccgcctcg cccgcgctgg cccatcagga cattgcgccc tacaccgatc
    15541 tgctcctcca cgacatgggg ccggggctcg ccgactggcg tccggatttt gcagcgggcg
    15601 gacgggagtg gcgcacgccg cccctgtggg gtctcggtct gaccgctgcg gtcagcgagc
    15661 gggcgcgcta ccttcacgac ggccgtgccc gcagcctgca cgaggccatc ctgtggcatg
    15721 ggggcgaggc caccgtggcg cggcagcgtt acgctgcctt gggtgccgcg ggtcgcgccg
    15781 agttgatggc ctttctggga tcgctgtgag ccggcccgga tggtctccgg tacgcaccgg
    15841 tctggggcac tatcgtgata tagcgctgca tataggtgcc acgggggcgg tccgcggcgg
    15901 cggcgtgact gaagctgccc caggctggaa ctggatttga tgcgctgcag taaatcaggt
    15961 gctgacgccg aggatcacgg agggcgcctt gaacaccgcg gtggctggtg cgcccagctt
    16021 cagccccagg gcaacggagc cctcgtgggt gatggtggca tacaccgtat gaccgtctgc
    16081 cagaccgata gccacctcgg tgctcaccgg gccttcggtc agggcggtga tcgtgccggc
    16141 cagacagttg cgcgccgaca gccggacgtc cgcgccttcc gtgaggacca gcaccgagga
    16201 cgccttgatg atggccttca ccgggcggcc cgggatcagc ccgagctcaa tggcgctgtc
    16261 gcgggtgacg gtcgcgacga tcacgatgcc accgggcagg gtgacatcca cttcggtggt
    16321 gactgcgccg ggtttgacgg cggtgacggt ccctccgaac acgttgcggg cactgatctt
    16381 catgaggcat ctcctgattt tggcaatctt gcactgcagc aaccttggcc actctacgcc
    16441 cgtgcacgcg gacccggcaa ggggctgttc accgattcga gatggagcgg aaattgctaa
    16501 agatcgctaa gatccgttgt gtacgacttt atacagagta ctcatgacca cgcgacgtcc
    16561 gattcaccac aaactcgtcg gaacgctgac cgtcgatacc gagttcggca cctttctggg
    16621 cgacacgcgc atccgtctgc tggaagccat cgagcgttac ggctccatct cccaggcggc
    16681 gaaggccgtg cccctgtcct acaaggccgc gtgggatgcg gtggatgcca tgaacaacct
    16741 ggccgaggaa cccctggtgg agcgctcggt gggcggcaag cacggcggcg gtaccagcct
    16801 taccgagtac ggccagcggg tcatcgccct gtatcgggcg gtggaactgg aataccagca
    16861 ggccatcgac cgcctcgcca agaccctggg cgagccgggc gccggttcgg tgcgccagtt
    16921 ccagaccctg ctgcggcgca tgtccatgcg caccagcgcg cgcaaccagt ttgtcggcac
    16981 cgtgtccggc ctgcgcgaag gggaggtgag tttcgaggtg cgggtgcgcc tcgacgacgc
    17041 ccacgaaatt gtcgcggtca tcacccgcga gagcgccgaa aacctggagc tggccatcgg
    17101 tctggaggtg cacgccttcg tcaaggcgcc gtcggtgatc atcgtcaccg atcccgagac
    17161 ccgcacctcc gcccgcaatc acctgtgggg cgaagtgacc cgcatccatc cgggcccggt
    17221 cagttcggaa gtcaccatca ccctgcccag cgggcgcaac gtcacttcgg tggtgaccca
    17281 cgacagcatc gacatgctgg ccctcgcgcc gggggtgccg gcctgcgccg tgttccaggc
    17341 gtccgccgtg atcctcgccg ttttcgactg atcccattcc gaaagggtct cccatgaagc
    17401 acacgttccg acatgcatcc ctgttcctcg tcctcgccgg cgccgcgctg ctggcccgtg
    17461 cggaggagat ctccgtcgcc gtggcggcca acttcaccgc gccgatgcag aagatcgccg
    17521 ccgattttga gaaggccacg ggccacaagg cccagctggt gtccggctcc accggcaagt
    17581 tctacgcgca gatcaaatcc ggcgcgccct tccagctgct cctcgccgcc gacgacgaga
    17641 cgccggcccg cctgatcaag gaaggtgacg gtgtgagcgg cagcgccttc acctacgcca
    17701 tcggcaagct ggtgctgtgg tcgcccaagc ccggcttcgt ggacgacaag ggcgaggtgc
    17761 tcaaggccgg ccgcttcgag cacctgtcgg tggccaatcc caagctggcg ccttacggcc
    17821 tggcggcgat ggaagccctc aaggccctca acctggccga tgccctgcag ccgaaagtcg
    17881 tgctcgccga aagcatctcc caggcccagc agtttgtcac ctcgggcaac gccgagctgg
    17941 gcttcatcgc ctattcccag atccacaagg acggcaggat catcgacggc agctactggc
    18001 tggtgcccgc gcgcctgtac ggtccgatcc gccaggatgc ggtgatcctc gcccagggca
    18061 aaggcaatgc cgccgtcgag gcgctgacgg cctacctcaa gtcgcccgcc gccgccgcgg
    18121 tgatccgcag ctacggctac gaccggtaag gcttttgccc aaccgacctc aagggaaatg
    18181 ccgtggaact cggtcccgac gatctgaatg ccttgctcct gacggcgaag ctggctaccg
    18241 tcgtcaccct ggcgctcctg gtggtgggca cgcccatcgc ctggtggctg gcgcggaccc
    18301 ggtcgcgcct gcggggcgtg atcggtgcat tcgtctcgct gcccatcgtg ctgcccccga
    18361 cggtcctcgg tttttatctg ctgctgctca tggggccaca aggcctcgtc gggcagacta
    18421 cccgcgccct cgggctgggc atgttgccct tcagttttgc cgggctcgtg gtggcttcgg
    18481 tgttctattc cttgcccttc gtcgtccagc ccatccagaa cgcctttctg gcgatgggcg
    18541 aacggcccct cgaagtcgcg gccacgcttc gcgcgtcgcc ctgggaaagc ttcttccggg
    18601 tcgccctgcc ccaggcgcga cagggctttg tcacggccgc cgtcctgggg ttcgcccata
    18661 ccgtcgggga gttcggggtg gtcctgatga tcggcggcaa cattcccggc aagacccagg
    18721 tcttgtcggt gcagatctat aaccacgtcg aagcattgga gtacggcaag gcccacgggc
    18781 tggcggccgg actcgtggcc ttctccttcc tggtcctgct gctgctccag gccctgggcg
    18841 gaggccggaa cacgaccggg gtggggcgat gagcgcgctc tcccttgtcc tgcggctgcc
    18901 cctcgccgac ttcgaactgc atgctgagct ggcgctgccc ggacgtggcg tgaccgccct
    18961 gttcggtcct tcgggttccg gcaagaccac cctgctgcgc tgcgtcgccg gcctggcccg
    19021 cggcgccgga cgtatcgtcg tgaatgacga agtctggcag gacgacgccc gccggatctt
    19081 cctgcccacc caccgccgcg ctctcggcta cgtcttccag gaagccagcc tgttcgagca
    19141 cctgaccgtg cgcggcaacc tggaattcgg cctgcgccgc atcctgcccg cggagcgcaa
    19201 ggtgagctgg gaccaggcgc tcgatctgct gggcatcggc ccgctcctcg agcggcgcag
    19261 caccaccctt tccggcggtg agcgccagcg ggtcgccatc gcccgggcgc tcctcaccag
    19321 cccgcgcctg ctcctgatgg acgagcccct cgcggcgctg gacgcgccgc gcaagcagga
    19381 gatcctgccc tacctggaac gcctccacga cgaactggcg attcccgtgc tctacgtgag
    19441 ccattccgcc gacgaggtgg cgcgcctggc cgatcacctg gtgctgctgg agcgcggccg
    19501 cgtggtggcc agcggcgcca ccgacgccct gctggcgcgt ctcgacctgc ccctggcgcg
    19561 ggacgaggag gccgcggtgc gcctcgacac catggtcctc gaccacgatg aggcctacgc
    19621 gctcaccacc ctgggttttg ccggcggcac catccaggtc acccgcaagg cgctgccggt
    19681 gggcgcccgg gcgcgcctca agatccaggc ccgtgacgtg agcatcgccc tgcgcggcga
    19741 caacccgtcg agcatcgtca atcgcgtgcc ggcgcgtgtc gtcggtttcg gcgaggcggc
    19801 ccacccggcc cagtgtctgg tggccctgga ggcgggcagg gtgcgcctga tggcacgcat
    19861 cacgcgcctg tcgcgggacc agctcggcct cgcggcgggc atggaggtgg tggcccagat
    19921 caagtccgtc gcgctgctgg cctgatccgg cgcaggtgcc cctcggcggc cgccaccggc
    19981 cgccgccgga cggtgccccg gcgggaacga agttccgcca caatcgctcc atgccctcat
    20041 gagcccttgc ccgcgggagc ccgccatgga cacgccgaga atcgcccttg ccctcctggc
    20101 cgccagcctg gcgctgccgg ccgcggccat caacaaatgc cagacgccgg acgggcgcgt
    20161 cacctacacg gacggtccct gcgcggcggg ctccacccag accggccgcg tcgagcccct
    20221 gccgccggtc gatcctgccg accgggccga tgccctggcc cgctcgaagc aggtcgtgga
    20281 cgacgcccgc gccctcgacg ccaagcgcga cgccctcgca gccgagcggc agaaaagcta
    20341 cgacgccgag cgccgcgccg aagaagaagc caggctccgc cagcaagccg ccgacgaagc
    20401 cgccaccgcc aacctctacc cggtgccggt gatcgtcccg cgcgtgcgcg cgccggtcgt
    20461 accggtggtc aagcccagac ccaggacgga agacaccgac cggccagcgg tgatggggaa
    20521 ttatccggtg cggcgctaac aggccgatac gaagcccgtt attcgcaacg cgggcctttg
    20581 tccgcgtact tcacctttcg cgaccctcgc gaggccctcc actatccata gcgatacgca
    20641 gtcgggcgta tcctaaattt gatttactgt cttgaggcac tggacgtgta ggacgaagct
    20701 tgcgtaaaac gttcggtgga taagcgcact aattcgggca gtgaagaata ttccgtcagt
    20761 ttctttctaa gccagtcagc gtcacttgcc ccttctcgta acaccttttt caggcactcg
    20821 atctgaaggc tgcaaccgag atcgtgcgca tgtggtttca atgcttcgag cagatcgtcc
    20881 agcgtttcgc ctaaaacgag tgcgcggcgg ttgacggggt ctgcgagtcg tgcattgaag
    20941 ccgtatcttg acgcttggaa cttattatat ctcgccacta cggcctgcaa tgcaggcgtc
    21001 agtgtggggc ggttgatcaa aagatacgaa gcaaggcact gtgcaaatgc tgccatctct
    21061 gtcgcgcgat ctacatcaag gggcgtgtca cacacccgga cttcgacagt tccgtattct
    21121 ggttttgggc gaacgtccca ataaatatct tttatgcttt gtgcgacgcc gcacgcttta
    21181 aggagttcaa ggtgtctgac aaagtcatcc cagtcctgta tgtcgggggc ctgtccggaa
    21241 aggggaaatg cggatatcgc tgtaaggcgg gaggattgaa agcccgtgtc cgttgcatcg
    21301 agataggggg aggacgcgga caaggcaatg aaatatggca cgaaggtgtt gagcgcgtga
    21361 gtcagccata tggcatcatt gcccgatgca acgccaatgt gaatgtgctg cccgaacacg
    21421 gtgaactgtt tgctgagata gccgtagcga tcactcagga atgcgaagcg ctctcccgga
    21481 gatatgcgtc gttctggcca ccggtggaag gggtgcgttc caccgccgca ggtagcaaca
    21541 ttgttgcgcg cgcattgaag cacgagaagg ccgcgcaggg cattcaggtc ctgggcaatg
    21601 gctgtgacat ccgcacgcgg cctcgtgttg atctcgatca tgctctctgt gatttcgagt
    21661 ttgacgtcgc cgtgacgtcc atcgtttttg aggccgtcga ggaggtctgt ggccgcacgg
    21721 gtcagatcga aatctctgag agagactaat tgaagctcca gctcgacccc aagtgtgagt
    21781 gggcgacttc ttgcaaatga atgacacatc tgtgtcacaa aagatctcct ttgacatcag
    21841 gcggacattc acccgcggtg atcagcgcgt aacgacaaag ttgcggaccc acaaattcca
    21901 tcattgcggc tgccaggaga gggagcagca gggcctcgct gccgacttcc gggtagatgc
    21961 tggcaatttc cgaggccatg aaaatggcgg tcgcggaaag tggttgaatg cccagtccta
    22021 ccatgcggcg ctttttcatg gagaggtcac cttgcccgaa gagtgtcacc ccgatgaatt
    22081 ttgcgacgcc gcgtacaaca atcaggccga atgccaattg agccgttagc caatttaggt
    22141 ctttcagtgg aagcattgca ccagttatga caaataatcc aatcgcgaga atccagtgaa
    22201 cgttgggtaa ttcgatgtag gacaagactc tcttgcgatc aatgagatta agtgacacac
    22261 ccatcatgaa aaggctgaga aatactggga ccgagagtgt ccgagccagt ccgattgtaa
    22321 aaacggctgt tgcgatgagc gcgaatgtcc gaacgacggc gccggttgca attttcgatg
    22381 caagcagtag ggcaatgcgc gcaccgagca tgccaatccc tactccacct gccatgaccc
    22441 agagtgggtg agcgaccgca ttgagccaat tgccggattg ctccgcatgg acaatcccga
    22501 gtaccgcggc gaagacgaca aacgacaagg cacaatttat cgcagtgagc agtaaggttc
    22561 tctgtgcgac ctgcccttgc gcgcgatttt cctgggtggt cagtagaacg actgctggtg
    22621 ccgaagccat cgtgatcgcc gcagttgcgg cactccatcc ggcgctgagg tcggcggcaa
    22681 agtatgaaaa ggcaaacacc aggatcagtg aaaggcttga ttccgcaatg gcgctcggca
    22741 acagttctcg gttggttcgc aaccatttca cgtccaggct tcgccctgtt tcaaggagga
    22801 gcaaacccag cgccgtgtcg gcgagcggtt ccgccattga gagcgttcgt gcatcgagcc
    22861 agtcgagtgc gcctggacct gccagcgcac ccgccgctac gtagccgatg atccgcgaaa
    22921 gccccagcag acggtgcagc gcttcgccgc tgatcatgcc tgcaacaaga agcagggcag
    22981 acaagaccaa ctgatcgggt tgatctgcca gtgggtgcag gaaatacaat ttttcttgca
    23041 gtgccaccca cgtcgtggcg agcggattca tcgccgtggg atccgattgc acgtgcggtg
    23101 tggggcagat cgtttggctc tgagcccctc gacagattcg gtggcgtgtg gatacgagcc
    23161 acgggatgcc atcgaggcga ctgcccggcg gaacgtgtct tgcaacgtta ttcctgttga
    23221 ccgtgtctgt gggcggggtt gccatgatct gggcccactt cgtcgctcgt gtgcgcagga
    23281 gtcctctcgg atgaggtaac gcatgtcgat cttgacgacc atgtatattg gtataaatat
    23341 aatttacacc aatatacttg ccttgggttg gacagcgatc ggttcgtggt gcgatccgcg
    23401 ccaacagacg tgattgatcg ggcgtgacag gctcggtgcg tttcgtcaag ccgtctgagc
    23461 ccaggaggca actcgtaggc ggatccagtg aagagggggc acggatgtct gaggaccgcg
    23521 cgttaaggat tggcttgtct gctcggctgt tccatccgaa cggagatgca agaggacttc
    23581 attccaagac gctccaggta ctggagcaat ccatcgcaca gtgggtcatg tcccgagacg
    23641 tgctggtcgt catggtgcct tccatcgtca gggatggccc gattcatcgc agcaatatcc
    23701 gtttggggga ttacgcggcc tatctcgacg gcttggtgtt gcagggcgga gcagacgtga
    23761 gcccatatgc atacggcgaa cgtccgctgt gcgatgtctg ggagggagat cccctgcgcg
    23821 atgcctatga aatggaactg atcgaggctt ttgtcagtgt gggcaagccc atcctcggca
    23881 tctgccgtgg gatgcaactc atcaacgtgg cctttggcgg gtctctgtat caggatattc
    23941 caacgcaact gcctgatgcg gggagccact cggacgaagc gtacgaccag cacagccatg
    24001 ccgtgtcgtt ctgtgagggg gggtacttcg ctgaatggtt tcgaggggca ggcggtcagg
    24061 tcgtctcaat ccatcatcag gctatcaagt ccttggggcg gaatctcgtc gcggaagcgg
    24121 tgtcgccaac ggatggcgtc atcgaagcca tccggttggc atccgatcct ttcgtgttgg
    24181 gcgtgcaatg gcatccggaa ttccatcggc cagggagtga cagtcttctg gattgtgccc
    24241 ctttgctgga cgccttcctg acagcagccc gccgacatgt ctgaaaagtc gttcgctctg
    24301 aaacgccgaa ctggcggtct tcgctcgtca gtgccggtcg ttccgaagcc cacacaccgg
    24361 gcgtttttcc ttgtctgctg cgggctcgat gccgacgact gcgtctaagg atcctcttac
    24421 gcttggcctt ctcttttcgc agaggccacc atgcgtcgtt tcttctccgt attcgtcgtt
    24481 ttggcagttg tcgccgcacc ccccgtgtac gccgaccccg ccactgaggc catccagacg
    24541 cccccgtccg gcacctggcc gagcttcggg cgggattaca
//
LOCUS       DAOQPA010000041        24542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_965_length_24542_cov_17.077551, whole genome shotgun sequence.
ACCESSION   DAOQPA010000041 DAOQPA010000000
VERSION     DAOQPA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24542
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_24542_cov_17.077551"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..658
                     /locus_tag="PKX01_07730"
     CDS             188..658
                     /locus_tag="PKX01_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76051.1"
                     /translation="MPVLVENSALIELKPAKATGLGMTQLGVPVLDGTQVKKGTLHEF
                     LQSTGEGTVGRRYQNIRVTAVRTTEGGVESAKVFVAFEVFGDDNVPEGANGGFAGALL
                     KGDVVLLALPVTPLFLPYGRAWYENQAVFDVPLAVFDGAERLQFTARADSIRAI"
     gene            complement(669..1265)
                     /locus_tag="PKX01_07735"
     CDS             complement(669..1265)
                     /locus_tag="PKX01_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76052.1"
                     /translation="MPPPVPAMLTDADARARLATHPLPDNPWVVATDHAILIKADYTD
                     TIERLLRWAPRARWSARQRCWIVPLGSLELVRSILPELSRLAEAMQPQPMAAEANGSS
                     ALPADAIARDAAARRLFRDHARCLYGSDWQRETARALGRDEAALAGWLIGQGVMEAPP
                     EVLLAEMLALMRRRVTEIGAAADALERQLIASGIAPLS"
     gene            complement(1375..2565)
                     /locus_tag="PKX01_07740"
     CDS             complement(1375..2565)
                     /locus_tag="PKX01_07740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ76053.1"
                     /translation="MHGPTLLLLTCILLGMMTLALAAARSGNRHIPGLGAWTLSYLCG
                     FLGSLSFLFRPPEVSVTSVLLAQGLLFTLAYLNLAGARAYMGRRPFPAWSVGAMLVLL
                     LGVAAFFTTAVPNTPLRVGVLGAVCGALFLASAFTMARGTPQLYPARYFLALACAVHG
                     LILIVRPWLFEAGGALGVRVDGSFLLSEYALLESIVALVMMAFGVLMAAHAHTTEALR
                     QQAERDPLTGVYNSRAFAVLLDKAAHLAGRMGTPLTLLVVDLDNFKRINDTWGHRSGD
                     LALRHFVNIATRSLRTEDIMGRIGGEEFAICLPNTLPEHALSVAERLRADVVAHPALG
                     EHGTLAFTVSIGLARCSPDESPAAALHRADQAMYRAKQRGRNRVETLPASEPGPLEAM
                     PARS"
     gene            2762..3682
                     /locus_tag="PKX01_07745"
     CDS             2762..3682
                     /locus_tag="PKX01_07745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ76054.1"
                     /translation="MHALLAAIASAPRLTDAGRLFHGRGGLHPGCEHWTLDVYPPVWL
                     TTSFQPVDDAALAALGDALSARWAQLAPGEPLCWVHQTRDEVRADTRTMAGQVPAPHV
                     VSEAGARFYVHLPRGFNHGFFLDMAEGRRWVREHVAARPGARVLNLFAYTCAFSVVAL
                     QAGAGQVANVDMAGGALAIGQRNHLLNGLNGGASFLPHDIFASWGRLRRGGPYDLIVV
                     DPPSYQKGSFVAEKDYPRLMRRLPELLAPGGHALLCLNSPKLGMAFLQDGMAEQAPTL
                     GFVGRLPNPPAFADVSDERALKVLVYRAPD"
     gene            complement(3707..4360)
                     /gene="nfsB"
                     /locus_tag="PKX01_07750"
     CDS             complement(3707..4360)
                     /gene="nfsB"
                     /locus_tag="PKX01_07750"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HMZ76055.1"
                     /translation="MSLTRNVYTRYTTKAFDPARRIPPEVFAELETLLRFAPSSVNSQ
                     PWHFFIADDDAGKARIARATPAAYAYNEGKIRNASHVVVLAVRKDLDDAHLAALLEQE
                     DRDGRFPTREARANQEKARAFYVGLHRNELKDVQTWASKQVYLALGALLLGAATLELD
                     AAPLEGFDTQILDAELGLAERGLTSLVIVALGYHSGDDFNARLPKSRLPAERVITRL"
     gene            4483..5394
                     /locus_tag="PKX01_07755"
     CDS             4483..5394
                     /locus_tag="PKX01_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76056.1"
                     /translation="MTHLRDLEVLVQAAERGSLSAAARRLEITPAAASMALRRLEDAL
                     GVPLFLRSTRSLRLTEAGQIYLEHCRQALQLLADGRDALLTGQAALRGTLHLSLPSDL
                     GRNAVQAWLDDFLAAHPQLNLRLLLSDRLADIYRQPVDLALRYGSLPDSALIALPVAP
                     DNQRLLCAAPEWLARHGTPRHPQDLAGHECLCFLLGDAVHDHWRFFRDGEVQRVQVSG
                     PRVADDGDAVRRWAVAGRGIAYKSALDVADDLAAGRLVRLLPDWRGEPAPLHLLCADR
                     RQISPLVQALRAFLAERCRQRLASPGP"
     gene            5515..6849
                     /locus_tag="PKX01_07760"
     CDS             5515..6849
                     /locus_tag="PKX01_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HMZ76057.1"
                     /translation="METKRRFYHSLYFQVLTAIAIGVALGHFQPDTGAAMKPLGDGFI
                     KLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGGLALLYFEIVSTIALIVGLVIVNVIQ
                     PGAGMNVDPNTLDTKGIAAFTGPGKMQSTTEFLLGVIPSSVVDAFAKGDILQVLLFSV
                     FFGFALHKFGGRGTLVFDFIEKMSHVFFQIVNMIMKAAPIGAFGAMAFTIGKHGVGAL
                     AQLGQLMATFYLTCLVFIFCVLGLIAKFHGFSIWKFIKYIKEELFIVLGTSSSESVLP
                     RMMAKMENLGVKKTVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQATNTPMDLTQQITL
                     LAVLLLTSKGAAGVTGSGFIVLAATLSAVGGVPVAGLALILGIDRFMSEARALTNLIG
                     NGVATVVVGKWCKELDTNALNRHLNGETAQEADAPELVLDIQETRLPGSKAA"
     gene            7048..8013
                     /locus_tag="PKX01_07765"
     CDS             7048..8013
                     /locus_tag="PKX01_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMZ76058.1"
                     /translation="MRFPPRRPLLLWLVPLLLTSTDALAWGLYTHVYFAQLLLWAIPL
                     ADPALRRLARRHPRLVMAGACLPDVALTARSAGADRLGTSHHWPQLHALMDAARNDAE
                     RAVVLGYASHLMADIIAHNHFVPAHERLWFESDLTTHVAVEWAMDHHVGGHLFARPGP
                     LLHSEREALVPLMSRAFHTHPEQVGQALATLAQGETWLRASRLHGGLYHLARFVDRRV
                     RRRFNLYLAHTSRRLGQLDRLMEGHLPSWAAEVEPVAARARLTDAPRRLLRACLPLPE
                     DFFAGACRRHPVGEIVLPDLPQGDDAGPASALLRPPGAALLSQPL"
     assembly_gap    8036..8135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8425..9507
                     /locus_tag="PKX01_07770"
     CDS             8425..9507
                     /locus_tag="PKX01_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76059.1"
                     /translation="MKALPQLPLWTLVAPVLGWLLLAGTRLDLGDAYLAVLAAGLLIG
                     VLASVHHAEVVAHRVGEPFGTLVLAAAVTAIEVALIVTLMLAGGESAMALARDTVFAT
                     VMIILDGIVGLCLLVGGRRFHEQQFGLYGVNAALSTLAAFVFLTLILPNFATTVPGPY
                     YSNKQLGFVAIVSLVLYGTFLFVQTVRHRDYFLPANADHDESVHAAPPSRAVTLISAL
                     LLPVGLAAVVLIAKAISPAIEAGVARLGAPQALVGVIIAVIVLLPEGLAAVRAARLNR
                     LQTSLNLALGSALASIGLSIPTVAIVSIVNGWPVQLGLDEKSMVLLVLSMLIAGQSLG
                     TGRTTMMQGAVHLVVFAVYLFFTVFP"
     gene            9532..10002
                     /locus_tag="PKX01_07775"
     CDS             9532..10002
                     /locus_tag="PKX01_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HMZ76060.1"
                     /translation="MIDNPRLITADARLTAVVPLIVPRADIAQVMGPAIHEVIATLHA
                     QGHAPAGPCFSLHHCRPTEVFDFEVGFPVNAPVTPAGRVVASTLPGVRVAQTVYRGPY
                     EGLGAAWGAFMAWIAAQHLQTGPGLWECYAAGPEVSPNPADWITELNCPVSDRS"
     gene            9999..10310
                     /locus_tag="PKX01_07780"
     CDS             9999..10310
                     /locus_tag="PKX01_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76061.1"
                     /translation="MKSPRFAAALAAALLSGTAGAADLCHANCASERKDCRFTAAQTE
                     ASEKGELIHTGPHAYPPTDYASTADAANQRLEEARARRRERESGCEAAYQRCANDCTP
                     R"
     gene            complement(10347..14765)
                     /locus_tag="PKX01_07785"
     CDS             complement(10347..14765)
                     /locus_tag="PKX01_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76062.1"
                     /translation="MAAELPLGPLGADCEALLGVVDTGLLIADPDSGQVLAVNDAWVA
                     TCGIARAAALGRRQDALGLWGDERDLQRCAAALGAHGVVRDFPAVLQVRGAARRMQLG
                     GRHVTLGGRTLEVWSFRDMTAHDEARSGLERCDERLRLLQMGGRVGVWEWHPEQGEAW
                     WSPALEAMFGFHEGAARSPADFRGRVHPDDQAVLETAAAALRGTGSFAVEFRVIRAAN
                     DVAWISAHGRAVSGLDGRSMRVVGNAVVITERKRLELEAHDLLRMRNVILDNASVGIL
                     FVQDRRIAWANRHFAAMFGHDLGALIGCSMRGFYAHPDDYRSLGEEGYATLARGEPYR
                     REVEVQGRDGRRFWARLTGNALDAAVPHDGSIWIIEDVSERRRYEQMLHASEARLRDI
                     IEGTSDAIFIKNLDGSYRLANEAAARFVGLPLEEIVGRDDTALFGAEDARAIMARDRA
                     VLAAGKVSTVEETVTTADGCVRTFLATKGPMRDETGHVIGLFGISRDITDRRRAEDAL
                     RERESQLRSISDNLPGGMIYQLEVDAATGISRLVYVSSGIERVQGFTAQEVMENPDLL
                     IEQVIEEDRPRLLAAQQAAGSGPGPFQAEVRIRRPDGEIRWLSLRSAPRQTMDGRQVW
                     DGIALDVTETREAHRALMLAERNYQMLFSEMLNGFALHEIVCDAGGRPVDYRFLDMNP
                     AFERMTGLRAAEVIGRTVREVLPGTESYWIDTYGKVALDGTPVKYENFSAELGKHFEV
                     MAFRPSPGRFACMVLDISERKQAERERVQSGRALRLLSHCNFALVKAQSERQLLTDIC
                     RLFVETGGYAMAWVGVPEDDAGRRVRPVVSWGDRHGYLDTVSVSWDEAQATGRGPCGR
                     AVRSREMQISRNIPGAPEMAPWHGASQRSGFRSSLSIPLIHGETLFGVLSFYSTEEDA
                     FGVGEVALLKEVADNVAYGIHTLQVRLERDAAEAATVAKSAFLANMSHEIRTPLNAIT
                     GMAQLIRRGGLTPLQADQMGKLESAGTHLLEIINDILDLSKIEAGKFVLEDTAVDPAA
                     LVKRVAALLEPRIDPAQVSMQVNVPSLPAGLQGDPTRLQQAVLNYASNAVKFTEAGTV
                     TLGLRVQDEDADGVLLRFEVSDTGIGIEADVLPRLFSAFEQADNSTTRKYGGTGLGLA
                     ITRKLAHLMGGDAGVNSTPGEGSTFWFTARLRKGRPTAREGAPVTTVRAEDALRREFP
                     GAVVLVVDDEPINLEIAAALIEDAGLCVETAGNGREAVALAHAGRHAAVLMDMQMPEL
                     DGLEATRRIRALPGGGRVPVIAMTANAYAEDRERCLEAGMNDFLAKPVEPDRLFEMLL
                     HWLRAGATPVSGPEDEFVWSARYSVGVDLLDRQHRALLGLCAAARQCLARPPGAGEGP
                     DLHELLADMEHYADEHFRTEEALLAAAGFPYMDAQIADHQAYRESLADFLYSASFDRL
                     KAGTVEQFLLKWWVHHILEGDAAYAGWLARDATVGT"
     gene            complement(15162..18518)
                     /locus_tag="PKX01_07790"
     CDS             complement(15162..18518)
                     /locus_tag="PKX01_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76063.1"
                     /translation="MSAQQEDITAELLLLYELSLTVGRTLDAPATCRDFLRVLMARRN
                     LTSASVWWFEPPDGNDPAPDEAAALTLIHALPRHAVPAGTLPSGHALARWARTAQTPR
                     HIPAGTPEHGALCFGEGEAEGAIALFPLDGHGVLRLHSQVAATLGERTLGQLRAVVDK
                     LATAIEGARAYARVRREIDERRQAEARLSEALEELQGLSRAVPDILVRIDPAGRLRWA
                     NDQALAVTGLPLETLLALPATAHFDEADHARVEAAIAAVFAGGQVDLEVPLRTRLGLR
                     MYHFRGVRVQAAGGEAALLASGRDISQRVEDLRAVEESRNLLQTVLETIPSRVFWKDR
                     ASRYLGCNSLFAADAGLAGPAQVIGREDGDLAWRAAAETIRHEDCRILLSGVPIPTGD
                     RRQMTADNPRAWVRGSRLPLRDAGGRIIGVLGVYEDVTRQKQTETELQVSRERLEYAL
                     RGTNEGMWDWNTDTDEVYFSPRWSSMLGYRNDELPHRLDTWADLMEPGDRERTLQHAV
                     AYLNGRIPRFEVEYRMRHRDGHWVDILSRAVAVQDLSGGTPRARRLVGTHMDISERKA
                     LQLSLQRQAAFTRAVIDAEADGVAVFHAVTGGPGVRFTVWNPSIELLCGYSLEAMNVH
                     GPIGTVFRPRRPERARRRLAQILTGRHLTGVELALHARDGTRRIVQVVTSTVARDEDG
                     VHVLAVFHDVTARKAAEAELRRHRSHLEELVAARTADLSVAKEAAEAANRAKGTFLAN
                     MSHELRTPMNAIIGLTHMVERQSSDPAQCERLRKIATSAQHLLQLLNDVLDLSKIDAD
                     RLTLERAPFRLGTVLGAVESLVGERASAKRLGLIQRIAPQLQARELIGDALRLQQILL
                     NLAGNAIKFTTEGHVTLAASIERTAPEGLHVRFEVADTGIGIPPEARQRIFDPFEQVD
                     GSTTRQFGGTGLGLTICRRLVRLMGGDIEVTSQPGAGSTFAFTICLEQGAAGIPRPDG
                     GLRPYSGAEAEARLRNRHRAARVLLAEDDWINQEVAIELLQEVAGLEVDLAIDGAQAV
                     TLAARRAYAVILMDVQMPEMDGLEAARRIRALPAHGATPILAMTANVFEDDRRACYEA
                     GMNDFIPKPVNPDVLFATVLDWLEAPEGRI"
     gene            complement(18523..19620)
                     /locus_tag="PKX01_07795"
     CDS             complement(18523..19620)
                     /locus_tag="PKX01_07795"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HMZ76064.1"
                     /translation="MYLRFASPAHVAAELRQSAVAGESPLWLLCVADTELPRVPELLE
                     SCRGEGVRVCGGVFPGLIHGTRVVHNGLLALPLPGASQVAVADLSAAGPRWRTPPSGA
                     GAHSAYVLLDSLAAGIAAFLDDLFDRFGGSLRYVGAGAACRDLRRAPVVFTGDGLWAD
                     GALAVLTPEHMEVHVRHGWTRVLGPFVASRSRANSILELNWRPAAALYRQSVEALAPA
                     FAGRPVFPDLGAAFPLCIGREDSEDVIRDPMGETDDGGLAVLADVAEHSPLYIAHGTP
                     ESLVAAARQAVEACAAGPRPARYFISDCYSRALMLGAGFRHELAGVATALAALTDAVP
                     EGVLALGEIASGGTRLLEFYNKTFVIAASRP"
     gene            19825..20454
                     /locus_tag="PKX01_07800"
     CDS             19825..20454
                     /locus_tag="PKX01_07800"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HMZ76065.1"
                     /translation="MKPAPCIRTVAVALTGASGMAYGLRLTECLLASGCRVWLLVSQV
                     AQVVARQELDLTLPSRPAEAQALLTARFGAAEGQLQVFGRDEWFAPPASGSNPPDAMV
                     VCPCTMGSLAAIAAGLAGTLIERAADVVIKEGRPLILVPRETPFSALHLENMLRLARM
                     GVVILPANPGFYHRPQSVEALVDFVVARILDQLRVPHSLLPRWGEDATE"
     gene            20457..21053
                     /locus_tag="PKX01_07805"
     CDS             20457..21053
                     /locus_tag="PKX01_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HMZ76066.1"
                     /translation="MERVELPLFPLRTVLFPGGVLPLRIFEARYLDMAARCMKAGMPF
                     GVCLIQDGQEVGEAATPHSVGTTARIVSWDMPQQGVLLVTCRGERRFRIDARQTHADR
                     LQSAQVQFLDAADAAPTPIPAGRAGVLPLLKAIVSDAGDELFPPPHAFDDAAWVVRRF
                     AEVLPIPALARQRLLELDDPLQALEIVQTYLRQHQLAS"
     gene            complement(21088..22155)
                     /gene="mutY"
                     /locus_tag="PKX01_07810"
     CDS             complement(21088..22155)
                     /gene="mutY"
                     /locus_tag="PKX01_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMZ76067.1"
                     /translation="MKTHHSFAATLIAWQRIHGRHDLPWQGTRDAYRIWLSEIMLQQT
                     QVDTVLPYYGRFLARFPDLASLAAAPVEDVLALWSGLGYYARARNLHACAQAVMARHG
                     GVLPADPAQLAALPGIGRSTAAAVAAFAYGTRAAILDGNVKRVLTRVFGIDGYPGERD
                     VEQHLWALAERLLPAQDVDVYTQAQMDLGATVCTRARPACGRCPLAGECVARRDERVA
                     ELPAPRPRRAVVVREGRFAAILRDGQVLLERRPPRGIWGGLLSLPELPEDADTAAWVG
                     QRFGLDVVRVARLPDFRHVFTHFTLHLRPERVDVAASDGAPGAGQVWLRLDALADAAL
                     PAPVRRLLERLASEGTPTVAG"
     gene            22246..22737
                     /locus_tag="PKX01_07815"
     CDS             22246..22737
                     /locus_tag="PKX01_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ76068.1"
                     /translation="MTAFKNDPAVRAFRDTLGMFPTGVTVVTARGADGAPVGLTVSSF
                     NSVSLAPPLVVWSLSGHLACVPAFENAAVYAINVLAEDQQALSQRFASRADDKFAGVA
                     CTDGTDGVPLLAGCCAVMECRPFAVHPGGDHLVFIGEVIRFERDAERKPLVFQGGAYR
                     RLA"
     gene            22742..23527
                     /locus_tag="PKX01_07820"
     CDS             22742..23527
                     /locus_tag="PKX01_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ76069.1"
                     /translation="MPAPHWIESGGGTRGLLFLHGVSGGAEAARTLLPQLLPQGWRGL
                     AWDMPGYGASAPLDPLDFDTLADAIVTLLDAAGLPRAVLVGHSLGGMIALHTAARHPA
                     RVDALVLACCTPAFGAPAGPLQQAFLARRLGPLETGATMAELAADLIPAMAGPDADPA
                     LIGAHIALMTRIPPARYAAALRALVHFDARAALPGLTMPALAVAGRHDSVSTPRVVRQ
                     MAERLPAATYAELPAGHLAPFEAPEAFARLVRDFLTRLPDRPA"
     gene            23549..23920
                     /locus_tag="PKX01_07825"
     CDS             23549..23920
                     /locus_tag="PKX01_07825"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HMZ76070.1"
                     /translation="MDESPFKGKTGLSRVWNAFRYSMAGLAAAYRNEDAFRQEVWLSA
                     LLIPVALVLPASAVGKALMVASVLLVVIVELINSAVEAAVDRVSLERHHLAKRAKDIG
                     SAAVFVALVNVIAVWGLVLAG"
     gene            24047..>24542
                     /locus_tag="PKX01_07830"
     CDS             24047..>24542
                     /locus_tag="PKX01_07830"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ76071.1"
                     /translation="MLRPLRYLPHGIAAACALIAACAALAAEPLVLQPVQLKALGIET
                     AVAAEAAAGRGARLPARVSVPNAQLRVVSAPVEGLVESLAVAPGSTVRKGQEVARLAS
                     RAALELQRDALQADSQAALLKHSLSRDETLFREGLIPESRLQATRAAAAQAGAQAAER
                     RQALG"
BASE COUNT         3510 a         9237 c         8095 g         3600 t
ORIGIN      
        1 ggcgcaatgg cgtcttgaga ttacgatgcg ccccattccc ggccccacct ccccccaagt
       61 ccgccgccct ccccactggc accgaacctg cttccccacc tccgtcgccg cgcgggcgcg
      121 cctttcggcg tgtcgcgtgt cgcacacgcg ccctgtcctt gtcgcattcg ataccacgga
      181 gagcatcgtg ccggtccttg ttgaaaattc cgccctgatc gaactgaaac ccgccaaggc
      241 caccggcctg ggcatgaccc agctgggggt gcccgtgctc gacggcacgc aggtgaaaaa
      301 gggcacgctg catgaattcc tgcaatccac cggcgagggt acggtggggc ggcgttacca
      361 gaacatccgg gtgacggcgg tgcgaaccac cgaggggggc gtggagtcgg ccaaggtctt
      421 cgtggccttc gaggtcttcg gcgacgacaa cgtgccggaa ggcgccaacg gcggctttgc
      481 gggggcgctg ctgaagggtg acgtggtcct gctggccctg ccggtgacgc ctctgttcct
      541 gccctacggc cgcgcctggt atgagaatca ggcggtcttc gacgtgcccc tcgcggtctt
      601 cgatggggcc gaacggctgc aattcaccgc ccgggccgat tcgatccggg cgatctgaac
      661 gcccggcctc agctgagcgg cgcgatgccg ctggcgatga gctgccgttc cagcgcgtcg
      721 gcggcggcgc cgatctcggt gacgcggcga cgcatcagcg ccagcatctc ggcgagcagc
      781 acctcaggcg gcgcctccat caccccctgc ccgatcagcc agccggccag ggcggcttcg
      841 tcgcggccca gcgcccgcgc ggtctcccgc tgccagtcgc tgccgtagag acagcgtgcg
      901 tggtcgcgga agagcctgcg cgcggccgcg tcacgggcga tggcgtcggc cggcagcgcg
      961 ctgctgccgt tcgcctcggc ggccatcggt tgcggctgca tggcctcggc cagacggctc
     1021 agttccggca ggatggagcg caccagttcc aggctgccca ggggcacgat ccagcagcgc
     1081 tggcgggcgc tccagcgcgc cctgggcgcc cagcgcagca ggcgttcgat ggtgtcggtg
     1141 tagtcggcct tgatgaggat ggcgtgatcg gtggccacca cccagggatt gtccggcaga
     1201 ggatgggtgg cgaggcgggc acgggcgtcg gcgtcggtga gcatggcggg cacaggcgga
     1261 ggcattggca cgctgcaagc aagttgggcg ccaggaatcc ggcggccccg ggcggtgggg
     1321 ctgggtgccc ggcccctgtc gcggcgcacc gtacgtgtcg actcgcggag cggctcagct
     1381 gcgcgccggc atcgcctcca gcggaccggg ctcgctggcg ggcagggttt ccacccggtt
     1441 gcgcccccgc tgcttggcgc ggtacatggc ctggtcggcc cggtgcaggg cggccgccgg
     1501 cgactcgtcc gggctgcagc gggccaggcc gatgctgacc gtgaaggcca gggtgccatg
     1561 ttcgcccagc gcggggtggg ccaccacgtc ggcgcgcagg cgttccgcca cgctgagggc
     1621 gtgctcaggc agcgtgttgg gcaggcagat cgcgaactcc tcgccgccga ttcgccccat
     1681 gatgtcctcg gtgcgcaggc tgcgggtggc gatattcacg aaatggcgca gggcgaggtc
     1741 gccgctgcgg tgcccccagg tgtcgttgat gcgcttgaag ttgtccagat ccaccaccag
     1801 cagggtcagg ggcgtgccca tgcggccggc cagatgggcg gccttgtcca gcaggaccgc
     1861 gaacgcgcga ctgttgtaga cgcccgtcag cggatcgcgc tcggcctgct ggcggagggc
     1921 ctcggtggtg tgggcatggg ccgccatcag aacgccaaag gccatcatga ccagggccac
     1981 gatggactcc aggagggcgt attcggacag caggaagctg ccatccaccc ggacccccag
     2041 ggcgccgccc gcctcgaaca gccacgggcg gacgatcagg atgaggccgt gcacggcaca
     2101 ggccagggcc agaaagtagc gggccgggta cagctggggc gtgccccgcg ccatcgtgaa
     2161 ggcgctggcc aggaacaggg ctccgcacac ggcgcccagc acgcccaccc gcagcggggt
     2221 attgggaacc gccgtggtga aaaacgcggc cacgcccagc aacagcacca acatggcgcc
     2281 cacgctccac gcgggaaagg gacgtcgccc catgtacgcc cgggcgccag ccaggttcag
     2341 gtaggccagc gtgaagagca agccctgcgc cagcaggacg gaggtcaccg acacctcggg
     2401 tgggcggaac agaaagctca ggctgcccag aaagccgcac agatacgaga gcgtccacgc
     2461 gcccagcccc ggaatatggc ggttgccgct gcgggccgcc gccagggcca gcgtcatcat
     2521 gcccagcagg atgcaggtca ggaggaggag cgtggggccg tgcatggggg atcgcctgag
     2581 ggccggacgg gcgtcgatgg gacagggaag ggcacgatgc gggcttcacc tgccgacgtc
     2641 aagcaccgga tgtcgcggat cgggggaaat tctgcaggcg gcccgcatcc aggcgccgtg
     2701 gaccggtcga acagcccgtg cccggtgtag gatgcgcgcc gtccttcccg tccctgcgcc
     2761 catgcacgcc ctcctcgccg ccatcgcctc cgcgccccgc ctgaccgacg ccggacggct
     2821 gtttcatggc cgcggcggcc tgcatcccgg gtgcgagcac tggacgctgg acgtctatcc
     2881 ccccgtgtgg ctgacgacca gcttccagcc ggtggacgac gccgcactgg ccgccctcgg
     2941 ggacgccctg agcgcccgct gggcgcagct ggcgccgggc gagcccctgt gctgggtgca
     3001 tcagacccgc gacgaggtgc gcgccgacac ccgcacgatg gccggccagg tgccggcgcc
     3061 ccacgtggtg agcgaggccg gcgcgcgctt ttacgtgcat ctgccgcgag gtttcaacca
     3121 cggcttcttt ctggacatgg cggaaggccg acgctgggtg cgcgagcacg tggcggcgcg
     3181 gccgggcgcc agggtgctca acctcttcgc ctacacctgc gccttctcgg tggtggccct
     3241 gcaggccggc gcgggacagg tggcaaacgt ggacatggcc ggcggcgcgc tggccatcgg
     3301 gcagcgcaac cacctgctca acggcctcaa cggcggggcg agcttcctgc cccacgacat
     3361 cttcgcctcc tggggacgcc tgcgccgcgg cggaccttat gacctgatcg tggtcgatcc
     3421 acccagctac cagaagggca gcttcgtcgc cgagaaggac tatccgcgcc tgatgcgccg
     3481 tctgccggaa ctcctcgccc ccggcggcca cgccctgctg tgtctcaact cgcccaagct
     3541 gggcatggcc ttcctgcagg atggcatggc cgaacaggcg cccacgctcg gtttcgtcgg
     3601 acgcctgccc aatccgccgg ccttcgccga cgtatcggac gagcgggcgc tgaaggtgct
     3661 ggtctaccgg gcgccggact gagcaccgcg gcgggcggcg gtgggatcag agccgcgtga
     3721 tgacccgctc cgcaggcagg cgggacttgg gcaggcgggc attgaagtca tcgccgctgt
     3781 ggtagcccag ggcgacgatg acgaggctgg tcaggccgcg ttcggccagt cccagctccg
     3841 cgtcgaggat ctgcgtgtcg aagccctcca gcggcgcggc gtcgagttcc agcgtggcgg
     3901 cgcccagcag cagcgccccg agggccaggt agacctgctt cgaggcccag gtctgcacgt
     3961 ccttcaactc gttccggtgc agtccgacgt agaaggcgcg ggccttttcc tgattggcgc
     4021 gggcctcgcg tgtggggaag cggccgtccc ggtcctcctg ctccagcagc gcggccaggt
     4081 gggcgtcgtc cagatccttg cgcaccgcca gcacgaccac gtgggaggcg ttgcggatct
     4141 tgccctcgtt gtaggcgtag gcggccggcg tggcgcgggc gatgcgcgcc ttgccggcat
     4201 cgtcgtcggc gatgaagaaa tgccagggct gggaattcac cgacgacgga gcaaagcgca
     4261 gcagggtttc cagttcggca aacacctcgg gcggaatacg ccgggcgggg tcaaaggcct
     4321 tggtggtgta gcgggtgtag acgttgcggg tgaggctcat gacgaaatcc ttgttcgaat
     4381 ggacgcgccc atcatgcgcc gcctcgcgct ttcaaaaaac tacaatgctg ttgattcact
     4441 ttcaagatat tctgaagaat cttcccctcc ggaacgccat ccatgaccca cttgcgcgac
     4501 ctggaagtgc tggtccaggc ggccgagcgc ggcagcctgt cggccgcggc gcgccggctg
     4561 gagatcaccc cggcggcggc gagcatggcc ctcaggcgcc tggaggacgc cctcggcgtc
     4621 cccctgttcc tgcgctccac ccgcagcctg cgcctcaccg aggcgggcca gatctacctc
     4681 gagcactgcc gccaggccct ccagctgctg gccgacgggc gcgacgcgct gctcaccggg
     4741 caggcggccc tgcgcggcac cctgcacctg tccctgccct ccgacctggg ccgcaatgcg
     4801 gtgcaggcct ggctcgacga cttcctcgcc gcacatccgc agctgaacct gcgcctgctc
     4861 ctcagcgacc gcctcgccga catctatcgc cagcccgtgg acctggccct gcgctacggc
     4921 agcctgccgg attccgccct gatcgccctg ccggtggcgc ccgacaacca gcgtctgctg
     4981 tgcgcggcgc ccgaatggct ggcccgtcac ggcaccccgc gccacccgca ggatctggcc
     5041 ggccacgagt gcctgtgttt cctgctgggc gacgcggtgc atgaccactg gcgtttcttt
     5101 cgcgacggcg aggtgcagcg cgtgcaggtg tccggcccgc gggtggcgga cgatggcgac
     5161 gccgtgcggc gctgggcggt ggccggccgg ggcatcgcct acaagtcggc actggacgtg
     5221 gccgacgatc tggccgccgg gcgactggtg cgcctgctgc ccgactggcg cggcgaaccg
     5281 gccccgctgc atctgctgtg cgccgaccgg cgccagatca gcccgctggt gcaggccctg
     5341 cgtgccttcc tcgccgagcg ctgccggcag cggctggcgt cgcccggtcc ctgaaaccgc
     5401 gcgaccggct gcctgccgcg gcccgattgc ggctttccac aatggggatg gacacaattc
     5461 atttccccct ccgccgtgcc tacactggaa tcatcaaaaa ccgggggaaa gaccatggag
     5521 acaaaacgcc gcttctatca ttcgctgtac ttccaggtgc tcaccgccat cgccatcggc
     5581 gtggccctgg ggcacttcca gcccgacacc ggcgcggcca tgaagccgct cggcgacggc
     5641 ttcatcaagc tcatcaagat gatcatcgcg ccgatcatct tctgcaccgt cgtggtcggc
     5701 atcgccggca tggaagacat gaagaaggtg ggcaagaccg gcggcctcgc cctgctgtac
     5761 ttcgagatcg tctccaccat cgccctgatc gtgggtctgg tcatcgtcaa cgtgatccag
     5821 ccgggcgccg gcatgaacgt ggatcccaac acccttgaca ccaagggcat cgccgccttc
     5881 accgggccgg gcaagatgca gtccaccacc gagttcctcc tcggcgtgat cccctccagc
     5941 gtcgtggatg ccttcgccaa gggagacatc ctgcaggtgc tgctgttctc ggtgttcttc
     6001 ggcttcgccc tgcacaagtt cggcggtcgc ggcacgctgg tcttcgactt catcgagaaa
     6061 atgtcccacg tcttcttcca gatcgtgaac atgatcatga aggccgcccc catcggcgcc
     6121 ttcggggcca tggccttcac catcggcaag cacggcgtcg gcgccctcgc ccagctcggc
     6181 cagctcatgg ccaccttcta cctgacctgt ctggtcttca tcttctgcgt gctgggcctc
     6241 atcgccaaat tccacggctt ctcgatctgg aagttcatca aatacatcaa ggaagagctg
     6301 ttcatcgtgc tcggcacctc gtcgtccgaa tccgtgctgc cgcgcatgat ggccaagatg
     6361 gagaacctgg gcgtcaagaa gaccgtggtc gggctggtga tccccaccgg ctactccttc
     6421 aacctggacg gcacggcgat ctacctcacc atggccgccg tgttcatcgc ccaggccacc
     6481 aacaccccga tggacctgac ccagcagatc accctgctgg ccgtgctgct cctcacctcc
     6541 aagggcgcgg cgggcgtcac cggcagcggc ttcatcgtgc tggccgccac cctcagcgcc
     6601 gtgggcggcg tgcccgtggc cggtctggcc ctgatcctcg gcatcgaccg cttcatgtcc
     6661 gaagcccgcg ccctcaccaa cctgatcggc aacggcgtcg ccaccgtggt ggtgggcaag
     6721 tggtgcaagg aactggacac caatgccctg aaccgccacc tcaacggtga aaccgcccag
     6781 gaggccgatg cccccgagct ggtgctggac atccaggaaa cccgcctgcc tggctccaag
     6841 gccgcctgaa ccatcgctca acgatagacc gaccctgaca gcgctcggcc cggcggagac
     6901 cccgtcgggc cgagtcgctt ttggcccccc tgcgccagcg ccgggggcca ccccgccagc
     6961 cgatctaaac caaaccgcaa ccgcgacgtc acccgcctga cactccggct gcacacgcac
     7021 gtcatccgcg cccggcacag tggcgcgatg cgtttcccgc cccgtcgccc cctcctgctg
     7081 tggctcgtgc ccctgctcct caccagcacc gacgcgctgg cctggggcct ctacacccac
     7141 gtgtatttcg cccagctgct gctgtgggcg atcccgctgg ccgacccggc cctgcgtcgc
     7201 ctggcccggc gccatccgcg cctggtgatg gccggcgcct gtctgccgga cgtggccctc
     7261 accgcccgca gcgccggcgc cgaccgcctc ggcaccagcc accactggcc ccagctccac
     7321 gccctgatgg acgccgcccg gaacgatgcc gaacgcgccg tggtcctggg ctacgccagc
     7381 catctgatgg ccgacatcat cgcccacaac cacttcgtgc cagcccacga gcgcctgtgg
     7441 ttcgaatccg acctcaccac ccatgtggcc gtcgaatggg ccatggatca tcacgtgggc
     7501 ggccacctct tcgcccgccc gggcccgctg ctgcacagcg agcgggaagc gctcgtaccg
     7561 ctgatgtccc gcgccttcca cacccatccg gagcaggtcg gccaggccct ggccaccctc
     7621 gcccagggcg agacctggct gcgcgccagc cgcctccacg gcggcctcta ccacctcgcc
     7681 cgttttgtcg accggcgcgt gcggcggcgc ttcaacctct accttgccca cacctcgcgt
     7741 cgcctcggcc agctcgaccg cctgatggaa ggccacctgc cgagctgggc cgccgaggtg
     7801 gaacccgtcg ctgcccgcgc ccggctgacc gacgcaccgc gccgcctgct gcgcgcctgc
     7861 ctgcccctgc cggaagactt cttcgccggt gcgtgccgcc gccacccggt cggcgagatc
     7921 gtcctgcccg acctgccgca gggcgacgac gccgggccgg cctccgccct cctccgccca
     7981 cccggagcgg ccctcctcag ccagcccctg tagtccggcc ctgggagtcc ggcccnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtcc ggccctggga gtccggcccc
     8161 cgcccggcgc acgccacgcc gtcgaggcgt accgatcccc gcgcggttct gataccttcc
     8221 caccctgagg ccggacgccc ccgcgatgat gcgggaatgc ccgccctccg gactttgcgg
     8281 agcacccgat ggatgcccga cctgtggccg gcgaggtgtt gccgcccggt cggcccgcgc
     8341 catcgggtgc gaccgcccgt atcggtcggc cgatcctgcc ccgccgcagg ccggtcgtcc
     8401 atcgcccgcc catcaggaca cccgatgaaa gccctgccgc aactccccct ctggaccctc
     8461 gtcgcccccg tgctgggctg gctactcctg gccggcaccc gcctcgacct gggcgacgcc
     8521 tacctggccg tgctcgccgc cggcctgctg atcggtgtgc tcgcctccgt ccatcacgcc
     8581 gaagtggtcg cccaccgggt cggcgagcca ttcggcaccc tggtgctcgc cgcggcggtc
     8641 accgccatcg aggtggccct catcgtcacg ctcatgctcg ccggcgggga atccgccatg
     8701 gccctggccc gcgacacggt gttcgccacc gtgatgatca tcctcgatgg catcgtcggc
     8761 ctgtgcctgc tggtgggcgg gcggcgcttc cacgaacagc agttcggcct gtacggcgtc
     8821 aacgccgcgc tgtccaccct ggccgccttc gtcttcctga ccctgatcct gcccaatttc
     8881 gccacgaccg taccgggccc ctactacagc aacaagcagc tcggtttcgt ggccatcgtg
     8941 tcgctggtcc tctacggcac cttcctgttc gtccagaccg tgcgccaccg ggattacttc
     9001 ctgccggcca acgccgacca cgatgaatcc gtccatgccg cgccaccgag ccgggccgtc
     9061 accctcatca gcgccctgct gctgccggtc ggcctggcgg ccgtggtcct gatcgccaag
     9121 gccatttcac ccgccatcga agccggtgtg gcccgcctgg gcgccccgca ggccctggtg
     9181 ggggtgatca tcgcggtcat cgtgctgctg cccgaaggcc tcgccgccgt ccgcgccgcc
     9241 cgcctcaacc gcctgcagac cagcctcaac ctggccctcg gatcggcgct ggcctccatc
     9301 ggcctgtcca tccccaccgt cgccatcgtc tccatcgtca acggctggcc ggtccagctg
     9361 ggcctcgacg agaagtccat ggtgctgctg gtgctctcca tgctcatcgc cgggcagtcc
     9421 ctcggcaccg ggcggacgac gatgatgcag ggcgccgtcc acctggtggt gttcgccgtc
     9481 tacctgttct tcacggtctt tccctgaacg ctcaaacgcc cgacgtccat catgatcgac
     9541 aacccccgcc tcatcaccgc cgacgcccgc ctcacggccg tggtgcccct catcgttccg
     9601 cgcgccgaca tcgcccaggt gatgggcccg gccatccatg aagtcatcgc caccctgcac
     9661 gcccagggcc atgcgccggc cgggccgtgt ttctcgctgc atcactgccg ccccaccgag
     9721 gtcttcgatt tcgaggtggg ttttcccgtc aacgcgcccg tgacacccgc cggacgcgtc
     9781 gtcgccagca ccctgcccgg cgtgcgcgtg gcccagacgg tctaccgggg tccttatgag
     9841 ggcctcggag cggcatgggg tgccttcatg gcctggatcg ccgcccagca cctgcagacc
     9901 gggcccgggc tgtgggaatg ttatgccgcc gggccggaag tcagccccaa cccggcggac
     9961 tggatcaccg aactcaactg cccggtcagc gatcgatcat gaaatccccg cgcttcgcag
    10021 ccgcattggc ggccgcgctc ctgagcggca ccgccggcgc tgccgacctg tgccacgcga
    10081 actgtgcgtc cgaacgcaag gactgccgct tcaccgcagc ccagacggaa gccagcgaga
    10141 agggcgagct gatccacaca gggccccatg cctacccgcc gaccgattac gcgtccaccg
    10201 ccgatgcggc caaccagcgc ctcgaggaag cccgcgcccg ccgccgcgag cgggagtccg
    10261 gctgcgaagc ggcctaccag cgctgcgcca acgactgcac accccgctga atcggcaccg
    10321 cgggccgccc ccgcccggca cggggatcag gtgcccacgg tcgcgtcgcg ggcgagccag
    10381 cccgcgtagg ccgcgtcgcc ctccagaatg tggtgcaccc accacttcag gagaaactgc
    10441 tccacggtgc cagccttgag ccgatcgaag gaggccgaat acagaaaatc ggccagcgac
    10501 tcccgatagg cctggtgatc cgcgatctgg gcgtccatgt agggaaagcc ggccgcggcg
    10561 aggagcgcct cctcggtacg gaagtgttcg tcggcgtaat gctccatgtc ggccagcagc
    10621 tcatgcagat ccggcccctc gccggcaccc ggcggacggg cgaggcactg ccgtgccgcc
    10681 gcgcacaagc ccagcagggc gcggtgctgg cggtcgagca gatcgacgcc gacgctgtag
    10741 cgcgccgacc agacgaactc gtcctcgggt ccgctcaccg gcgtcgcacc cgcccgcagc
    10801 cagtggagca gcatctcgaa gaggcggtcc ggctccaccg gcttggccag aaaatcgttc
    10861 atgcctgctt cgaggcagcg ctcccgatct tccgcatacg cattggcggt catcgcaatg
    10921 accggcaccc gccctccgcc gggcagggcg cgaatgcgcc gggtggcctc cagcccgtcc
    10981 agttcgggca tctgcatgtc catcagcacc gcggcgtggc ggcccgcatg ggccagggcg
    11041 accgcttcgc ggccattgcc cgccgtctcc acgcacagcc cggcgtcctc gatcagtgcc
    11101 gcggcgatct ccaggttgat cggctcatcg tccaccacca gcacgacggc gcccggaaat
    11161 tcccgccgca gggcatcctc ggcccgcacg gtggtgacag gcgccccctc ccgggccgtc
    11221 ggccggccct tgcgcaggcg cgcggtgaac cagaaggtgc tgccctcgcc gggcgtgctg
    11281 ttcacgccgg catcgccccc catcaggtgc gccagcttgc gcgtgatggc caagcccagg
    11341 ccggtgccgc cgtacttgcg ggtggtgctg ttgtcggcct gctcgaaggc cgagaacaga
    11401 cggggcagca cgtcggcctc gatgccgatg cccgtgtcgc tcacctcgaa gcgcagcaac
    11461 accccgtcgg cgtcctcgtc ctgcacccgc aggcccagcg tgacggtgcc cgcctcggtg
    11521 aacttgaccg cgttgctggc gtagttgagc acggcctgct ggaggcgcgt cggatcgccc
    11581 tgcaggccgg ccggcaggga cggcacgttt acctgcatgc tcacctgcgc cgggtcgatg
    11641 cgcggttcca gcagggccgc aacccgcttc accagcgccg ccggatccac cgcggtgtcc
    11701 tccagcacga acttgccggc ctcgatcttc gacagatcca ggatgtcgtt gatgatctcc
    11761 agcaaatggg tgccggcgct ctccagcttg cccatctggt ccgcctgcag gggcgtgagg
    11821 ccgccgcggc ggatcagctg ggccatgccc gtgatggcat tgaggggcgt gcggatctca
    11881 tggctcatgt tggccaggaa ggcactcttg gccaccgtgg cggcctcggc ggcgtcccgc
    11941 tcgaggcgca cctgcagcgt gtggatgccg taggccacgt tgtcggccac ctccttgagc
    12001 agggccacct cgcccacccc gaaggcgtcc tcctcggtgg aatagaagct cagcacaccg
    12061 aacagggtct cgccgtggat cagcggaatg ctcaggctgg agcggaaccc ggaacgctgc
    12121 gacgccccgt gccacggcgc catctcgggc gcgcccggga tgttccggct gatctgcatc
    12181 tcccggctgc gcaccgcccg cccgcagggc ccccgtccgg tagcctgggc ctcatcccac
    12241 gataccgaaa ccgtgtcgag atagccgtgc cggtcgcccc aggacaccac cggccgcacg
    12301 cggcggccgg catcgtcctc gggtacgccc acccaggcca tggcgtagcc gcccgtctcc
    12361 acgaacaggc ggcagatgtc cgtgagcagc tgccgttctg actgggcctt gaccagggca
    12421 aagttgcaat gactgagcag gcgcagggcg cgcccggact ggacccgctc ccgctcggcc
    12481 tgcttgcgct cgctgatgtc gagcaccatg caggcaaaac ggcccggcga ggggcggaag
    12541 gccatcactt cgaagtgctt gcccagttcg gcggaaaagt tctcgtattt gaccggcgtc
    12601 ccgtccaggg cgaccttgcc gtaggtgtcg atccagtagg attcggtgcc cggcagcacc
    12661 tcgcgcacgg tgcgcccgat cacctctgcc gcgcgcaggc cggtcatccg ctcgaaggcc
    12721 gggttcatgt cgaggaagcg gtagtccacg gggcggccac cggcatcgca gacgatctcg
    12781 tgaagcgcga agccgttgag catctcgctg aagagcatct ggtaattgcg ctcggccagc
    12841 atcagggccc gatgggcctc acgggtctcg gtgacgtcca gcgcgatgcc gtcccatacc
    12901 tgacgtccgt ccatggtctg acgcggcgcg gagcgcaggg acagccagcg gatctcgccg
    12961 tcggggcggc ggatgcgcac ctcggcctgg aacggtcccg gcccgctgcc ggcggcctgc
    13021 tgcgccgcca gcaggcgcgg ccgatcctcc tcgatgacct gctcaatgag gaggtccggg
    13081 ttctccatca cttcctgggc ggtgaagccc tgaacccgtt cgatgcccga actcacgtat
    13141 accaagcgcg agatcccggt cgccgcatcc acctccagct ggtagatcat gccgccgggc
    13201 aggttgtcgc tgatgctgcg cagctgggac tcccgctccc gcagcgcgtc ctcggcacgg
    13261 cgccggtcgg tgatgtcccg cgagatgccg aacaggccga tcacgtgacc ggtctcgtcg
    13321 cgcatggggc ccttggtggc caggaaggtg cgcacgcaac cgtccgccgt ggtcacggtc
    13381 tcctcgaccg tgctgacctt ccctgccgcc agcaccgccc ggtcgcgggc catgatcgcg
    13441 cgggcatcct ccgcgccgaa cagcgcggtg tcgtcgcgac cgacgatctc ctccaggggc
    13501 agcccgacaa agcgcgccgc ggcctcgttg gcgaggcggt agctgccgtc cagattcttg
    13561 atgaagatgg cgtccgaggt gccttcgatg atgtcccgca ggcgtgcctc gctggcatgc
    13621 agcatctgct cgtagcgccg ccgctcgctg acatcctcga tgatccagat gctaccgtcg
    13681 tggggcaccg cggcatccag tgcattgccc gtgaggcgcg cccagaagcg ccggccgtcg
    13741 cggccctgca cctccacctc gcgccggtag ggctcgcccc gcgcgagcgt ggcgtacccc
    13801 tcttcgccga gggagcggta atcgtccggg tgcgcataga agccgcgcat gctgcagccg
    13861 atcagcgcgc ccaggtcgtg accgaacatc gccgcaaaat gccggttggc ccacgcgatg
    13921 cggcggtcct gcacgaagag gatgcccacg ctggcgttgt ccaggatcac gttgcgcatg
    13981 cgcagcagat cgtgggcctc cagttcgagg cgtttgcgct cggtaatgac gacggcgttg
    14041 cccaccacgc gcatgctgcg gccgtccagg ccgctcaccg cgcggccgtg ggcagagatc
    14101 caggcgacat cgttcgcggc gcggatcacc cggaactcca ccgcaaagct gccggtcccg
    14161 cgcagggccg ccgccgcggt ctcaagcacc gcctggtcat ccggatggac acgcccgcgg
    14221 aaatccgcgg ggctgcgcgc cgccccttcg tggaatccga acatcgcctc cagggccggc
    14281 gaccaccagg cctcaccctg ctccggatgc cactcccaca cgcccacgcg cccgcccatc
    14341 tgaagcagtc gcaggcgctc atcgcagcgc tcgagcccgc tccgcgcctc gtcgtgggcc
    14401 gtcatgtcgc ggaaggacca cacttccagt gtccgcccgc caagggtcac gtgccgcccg
    14461 cccagctgca tccgccgcgc cgcgccgcgc acctgcagga ccgccggaaa gtcccggacc
    14521 acgccgtggg cgcccagggc ggccgcgcag cgctgcagat cgcgttcgtc gccccacagg
    14581 cccagcgcgt cctgccgccg gccgagggcc gccgcgcgcg caatgccaca ggtggcgacc
    14641 caggcatcgt tcacggccag gacctgcccg gaatccgggt ccgcgatcag gagtcccgtg
    14701 tccacaacgc ccaacaaggc ctcgcaatcg gcccccagcg gcccaagggg aagctcagca
    14761 gccatgttcc ggcgctcccc agactgccat cgtttccatc gtccgtcctc cgccccctcg
    14821 tttcgcgtac ttcatcggcg ccgccccacc ggtctgaagc cggttgtggc gcgccgcgtc
    14881 gcccaggatc cgatcagacc ataaaaaacg ggccctgcgg tgccagcgaa ggcacgggag
    14941 ggcccgaaaa gacgaggagg agattcggca tgcggccatc cgagacaacc gcaccgaacg
    15001 ctttggacga tgcaagagcc attccacacc gtcccgcgcc cggaacggcc cgcgccacga
    15061 ccctccgcca ccctgccgca ggccccgaac tggccggaaa acgggccgga ctggtgcatc
    15121 cgccgtccat gcgcacccaa aatgggcacc ctgccggcga ctcatatccg gccctccggc
    15181 gcctccagcc agtccaggac tgtcgcaaac aacacatccg gattcaccgg cttggggatg
    15241 aaatcgttca tgccggcctc gtagcaggcg cgccggtcgt cctcgaacac gtttgcggtc
    15301 atggccagaa tcggggtcgc gccgtgtgcg ggcagggcgc ggatgcgccg cgccgcctcc
    15361 agcccgtcca tctcgggcat ctgcacgtcc atcaggatca ccgcgtaggc gcgccgcgcc
    15421 gccagggtga ccgcctgggc gccgtcgatg gccaggtcca cctccaggcc cgccacctcc
    15481 tgcagcagct cgatggccac ctcctggttg atccagtcgt cctccgccag cagcacgcgg
    15541 gccgcgcgat ggcggttccg caggcgcgcc tccgcctcgg cgccggaata gggacgcagc
    15601 ccgccgtccg ggcgcgggat gccggcggcc ccctgttcca ggcagatggt gaaggcaaag
    15661 gtgctgccgg caccgggctg gctggtcacc tcgatgtcgc cgcccatcag ccgcaccaga
    15721 cgccggcaga tggtgaggcc caggccggtc ccgccaaact gacgcgtggt ggagccatcc
    15781 acctgctcga aggggtcgaa gatgcgctgg cgcgcctccg gtggaatgcc gatgccggtg
    15841 tcggcgacct caaagcgcac atgcaggcct tcgggtgccg tccgttcgat cgaggccgcc
    15901 agggtgacat gcccttccgt cgtgaacttg atggcgttgc ccgccagatt gagcaggatc
    15961 tgctgcaggc gcagggcgtc gcctatcaac tcgcgcgcct gcaactgggg cgcgatgcgc
    16021 tggatgaggc ccaggcgctt ggcgctggca cgttcgccga cgaggctctc cacggcgccc
    16081 aggaccgtgc cgaggcggaa cggtgcccgc tcgagggtga ggcggtcggc gtcgatcttg
    16141 gacagatcga ggacgtcgtt gaggagctgc agcagatgct gggccgaggt ggcgatcttg
    16201 cgcaggcgct cgcactgggc cggatcgctg ctctgccgct ccaccatgtg ggtcaggccg
    16261 atgatcgcgt tcatcggcgt gcgcagctcg tggctcatgt tggccaggaa ggtgcccttc
    16321 gcccgattgg cggcctcggc ggcctccttg gccaccgaca ggtcggcggt gcgcgctgcc
    16381 accagctctt ccaggtggct gcggtgacgc cgcagctccg cctcggcggc cttgcgcgcg
    16441 gtgacatcat ggaacaccgc cagcacgtgg acgccatcct cgtcgcgggc aacggtggac
    16501 gtgaccacct gaacgatgcg ccgggtcccg tcgcgggcat gcagggccag ctccaccccc
    16561 gtcaggtgcc gtcctgtcag gatctgcgcc agacgccgcc gcgcccgctc cggacgccgc
    16621 gggcggaaga ccgtgccgat cggcccatgc acgttcatcg cttcgaggga atatccgcag
    16681 agcagctcga tgctcgggtt ccacaccgtg aagcgcacgc ccggcccccc cgtcaccgca
    16741 tggaacaccg ccaccccgtc cgcctccgcg tcgatgaccg cgcgggtgaa tgcggcctgc
    16801 cgctgcaggc tgagctgcag ggccttgcgc tcgctgatgt ccatgtgcgt ccccaccagc
    16861 cgtcgggcgc gcggggtgcc gccgctcagg tcctgcaccg ccacggcgcg ggacaggatg
    16921 tccacccagt ggccgtcccg gtgacgcatg cggtattcca cctcgaagcg gggaatgcgg
    16981 ccgttcaggt aggccaccgc gtgctgcagg gtacgttccc ggtcgcccgg ttccatcagg
    17041 tccgcccagg tgtccaggcg atggggcagt tcgtcgttgc ggtagccgag catggaggac
    17101 cagcgcggcg agaaatacac ctcgtcggtg tcggtgttcc agtcccacat gccctcgttg
    17161 gtcccgcgca gggcgtactc cagccgctcg cggctcacct ggagctcggt ctccgtctgc
    17221 ttctggcggg tgacgtcctc gtacaccccg agcacgccga tgatccgccc gccggcatcc
    17281 cgcaggggca ggcgcgatcc gcgcacccat gcccgcggat tgtcggcggt catctggcgg
    17341 cggtcgccgg tcgggatcgg cacaccgctc aacaggatgc ggcagtcctc gtggcggatc
    17401 gtctcggcgg ccgcgcgcca cgccagatcg ccatcctcgc ggccgatcac ctgcgccggc
    17461 ccggccagcc cggcatccgc cgcaaacagg ctgttgcagc ccagatagcg ggacgcacga
    17521 tccttccaga agacccggct ggggatggtc tcgaggaccg tctgcagcag gttgcgggac
    17581 tcctccaccg cgcgcaggtc ctccacccgc tggctgatgt cccgcccgga tgccagcagc
    17641 gccgcctcgc cccccgccgc ctgcacgcgc acgccgcgga agtggtacat gcgcagcccg
    17701 aggcgggtgc gcagagggac ttccagatcc acctgcccgc cggcgaagac ggccgcgatg
    17761 gccgcctcca cgcgggcgtg gtcagcctcg tcgaagtgcg ccgtggcggg cagggccagc
    17821 aaggtctcga ggggcaggcc ggtcacggcc agggcctgat cgttggccca gcgcaaccgg
    17881 cccgccggat cgatgcgcac gaggatgtca ggcaccgccc gcgacagccc ctgcagctcc
    17941 tccagcgcct cgctcaggcg cgcctcggcc tggcgccgct cgtcgatttc gcggcggacg
    18001 cgggcgtagg cgcgggcgcc ttcgatggcc gtggcgagct tgtccaccac cgcccgcagt
    18061 tggcccaggg tgcgttcgcc cagagtcgca gccacctggg aatgaaggcg cagcacgccg
    18121 tgaccgtcga ggggaaacag ggcgatcgcc ccctccgcct cgccttcgcc aaaacacagg
    18181 gcgccgtgtt ccggcgtccc cgccgggatg tggcgcggcg tctgggcggt ccgcgcccag
    18241 cgggccaggg catgcccgga cggcagtgtg cccgccggca ctgcgtgacg cggcagcgca
    18301 tggatcaggg tcagcgccgc ggcctcgtcc ggcgccgggt cgttcccgtc ggggggctcg
    18361 aaccaccaca cggacgcgga ggtcaggttg cgccgggcca tcagcacacg caggaagtcc
    18421 cggcacgtgg cgggcgcatc cagcgtgcga ccgacggtga gggacagttc atacaacagg
    18481 agcagctctg cggtgatgtc ttcttgttgt gcactcatgc ggtcagggcc gggatgcggc
    18541 gatgacaaag gtcttgttgt agaactccag caggcgggtt ccgcccgacg cgatctcgcc
    18601 cagggccagc accccctccg gcacggcgtc ggtgagcgcc gccagggccg tcgccacgcc
    18661 cgccagttcg tgccggaaac cggcgcccag catcagtgcg cgggaatagc agtccgagat
    18721 gaaataacgg gccggccgcg gcccggccgc acacgcctcc accgcctgac gggccgccgc
    18781 gacgaggctt tccggcgtac cgtgggcaat gtacagcggc gaatgctcgg ccacatccgc
    18841 cagcaccgcg aggccgccgt cgtccgtctc gcccatgggg tcacggatca cgtcctcgct
    18901 gtcctcgcgt ccgatgcaca gcggaaaggc cgcacccaga tcgggaaaca cgggccggcc
    18961 ggcaaaggcc ggcgccagcg cctccacgct ctgccggtag agggcggcgg ccggtcgcca
    19021 gttgagttcc agaatgctgt tggcgcggct gcgcgaggcc acgaacggcc ccaggacccg
    19081 ggtccagccg tggcggacgt gcacctccat gtgctcgggc gtgagcaccg ccagggcccc
    19141 gtcggcccac agaccgtccc cggtgaagac caccggcgcc cggcgcaggt cgcggcaggc
    19201 agcacccgcg cccacgtaac ggaggctgcc gccgaagcgg tcgaagagat cgtcgaggaa
    19261 ggcggcgatg ccggccgcca ggctgtccag cagcacgtag gcgctgtgcg ccccggcgcc
    19321 ggacggcggc gtgcgccagc gcgggccggc ggcgctcaga tccgccaccg ccacctggct
    19381 ggcacccggc aggggcagcg cgaggagacc gttgtgcacg acccgggtcc cgtggatcag
    19441 acccgggaac accccgccgc agaccctcac gccttcgccc cggcaggact ccagcagctc
    19501 cggcacgcgg ggcaactcgg tgtcggccac gcacagcagc cacaggggcg actcgccggc
    19561 cacggcggat tggcgcagct cggcggcgac gtgggcggga gacgcgaagc gcaggtacat
    19621 ggacgtatcc ggaacgggcg ggccggccgc cgcctccgga cgttccggac agggcggcga
    19681 ggcgcgatgc ggtgcattca cttctcaggc tagcagatgc accggcgatg caacatgcct
    19741 gatgccgtgt cgccccccgg tcgccgccca gcacgctttc ccgtatcctg cgaaccgatc
    19801 tcccccgcca cggtccgacc tgccatgaag cccgcgccct gcatccgcac cgtcgccgtc
    19861 gccctcaccg gcgcctccgg catggcctat ggcctgcgcc tcaccgaatg tctgctcgcc
    19921 tccggctgcc gggtctggct gctggtctcc caagtcgccc aggtggtggc ccggcaggag
    19981 ctggacctca ccctgcccag ccgcccggcc gaggcgcagg ccctgctcac cgcccgcttc
    20041 ggcgcggccg aagggcaact gcaggtgttc ggccgggacg aatggttcgc gccgcccgcc
    20101 tccggctcca acccgcctga cgcgatggtg gtgtgcccct gcaccatggg ctccctggcc
    20161 gccatcgccg ccggcctggc cggcacgctc atcgagcggg ctgccgacgt ggtcatcaag
    20221 gaaggccgtc cgctgatcct ggttccgcgg gagacgccct tctcggcgct gcacctggag
    20281 aacatgctgc gcctggcgcg catgggcgtg gtcatcctgc ccgccaatcc gggcttctac
    20341 caccgccccc agagcgtgga ggccctcgtg gatttcgtcg tcgcgcgcat cctcgaccag
    20401 ctccgcgtgc cccacagcct gctgccccgc tggggcgaag acgcaacgga gtgaacatgg
    20461 agcgcgtcga actgcccctc tttcccctgc gcaccgtcct gttccccggc ggcgtgctgc
    20521 ccctccggat cttcgaagcg cgctacctgg acatggcggc ccgctgcatg aaggccggaa
    20581 tgcccttcgg cgtatgcctg atccaggatg gccaggaggt gggcgaggcg gccacgcccc
    20641 acagcgtggg caccaccgcc cgcatcgtca gctgggacat gccccagcag ggcgtgctgc
    20701 tggtcacttg ccggggcgaa cggcgcttcc gcatcgacgc gcggcagacc cacgccgacc
    20761 gcctgcagag cgcccaggtg cagttcctcg acgcggccga cgcggcgccc accccgattc
    20821 ccgccgggcg ggccggcgtg ctgcccctgc tcaaggccat cgtcagcgat gcgggcgatg
    20881 agctgttccc gccgccccac gccttcgacg acgccgcctg ggtggtccgc cgcttcgccg
    20941 aggtgctgcc catccccgcc ctcgcccgcc agcgcctgct ggaactggac gatccgctgc
    21001 aggccctgga gatcgtccag acctacctgc gccagcatca gctggcaagc tgaagcgcgt
    21061 ccgcatccgt cgcaccacca cgcgggctca gccggcgacg gtcggtgtgc cttccgaggc
    21121 cagccgctcc agcagccgcc gcaccggcgc cggcagcgca gcgtccgcca gcgcgtccag
    21181 ccgcagccag acctgccccg cgccgggcgc cccgtcggat gccgccacgt ccacccgttc
    21241 cggacgcagg tgcagcgtga agtgggtgaa gacgtggcgg aaatcgggca ggcgcgcgac
    21301 acgcaccacg tccagcccga agcgctgccc cacccaggcc gcggtgtccg catcctcggg
    21361 cagctccggc aggctcagga gtccgcccca gatgccccgc ggcggacgcc gctccagcag
    21421 cacctgcccg tcccgcagga tcgccgcgaa gcgtccctcg cgcaccacga cggcacgccg
    21481 cggacgtggc gccggcagct cggcaacccg ctcgtcccgc cgcgccacgc actcgccagc
    21541 cagcggacag cgcccgcagg cgggccgggc gcgggtgcac acggtggcgc ccagatccat
    21601 ctgggcctgg gtgtagacgt ccacgtcctg cgccggcagc aggcgctcgg ccagcgccca
    21661 caggtgctgc tccacgtccc gctcgcccgg ataaccgtcg atgccgaaga cgcgggtcag
    21721 cacccgcttc acgttaccgt ccaggatcgc tgcccgcgtg ccgtaggcga aggctgccac
    21781 cgccgccgcg gtggagcggc cgatacccgg cagcgccgcc aattgcgccg gatccgccgg
    21841 caacacgccg ccgtggcggg ccatcaccgc ctgggcgcag gcatgcaggt tgcgggcacg
    21901 ggcgtagtag cccaggccgc tccacagggc cagcacgtcc tccaccggcg ccgccgccag
    21961 gctggccaga tccgggaagc gcgccaggaa gcgcccgtag tagggcagca ccgtatccac
    22021 ctgggtctgc tgcagcatga tttccgacag ccagatgcgg taggcgtccc gggtgccctg
    22081 ccagggcagg tcgtggcggc catggatgcg ctgccaggcg atcagcgtgg cggcaaaaga
    22141 gtggtgtgtt ttcaattcga taccccctcg acggcgacag gacgcagggc gataatgcgg
    22201 gcccccaaca ggtctgcggc gcctttgcag cgatccccac ccgccatgac agccttcaag
    22261 aacgatcccg ccgtgcgtgc cttccgcgac accctgggca tgtttcccac cggcgtgacc
    22321 gtcgtcaccg cccgcggcgc cgacggcgcg cccgtgggtc tcacggtcag ctccttcaat
    22381 tccgtgtccc tcgcgccgcc gctggtggtg tggagcctgt ccggccatct ggcctgcgtg
    22441 ccggccttcg aaaacgccgc agtgtacgcg atcaacgtcc tcgccgagga ccagcaggcg
    22501 ctctcccagc gcttcgccag ccgcgcggac gacaagttcg ccggcgtcgc ctgcaccgac
    22561 ggcaccgacg gcgtgcccct gctcgccggc tgctgcgccg tcatggaatg ccggcccttc
    22621 gccgtgcatc cgggcggcga ccacctggtc ttcatcggcg aggtgatccg cttcgagcgg
    22681 gacgccgagc gcaagcccct ggtcttccag ggcggcgcct accgccgcct cgcctgagtc
    22741 catgcccgcg ccgcactgga tcgagtccgg cggcggcacc cgcgggctgc tcttcctgca
    22801 cggcgtcagc ggcggcgccg aagcggcccg cacgctgctg ccgcagctcc tgccgcaggg
    22861 ctggcgcggc ctggcctggg acatgcccgg ctacggcgcc agcgcgccgc tggatccact
    22921 cgatttcgac accctcgccg acgccatcgt caccctgctg gacgccgccg gcctgccccg
    22981 cgccgtgctc gtcggccaca gcctgggcgg catgattgcc ctgcacaccg ccgcccgcca
    23041 cccggcccgc gtggacgccc tggtgctggc ctgctgcacg ccggccttcg gcgccccggc
    23101 cgggccgctt cagcaggcct tcctggcccg ccgcctgggc cccctggaga ccggcgccac
    23161 gatggccgaa ctcgccgccg acctgattcc cgccatggcc gggccggacg ccgacccggc
    23221 gctgatcggc gcccacatcg cgctgatgac gcgcatcccg cccgcccgct acgccgccgc
    23281 cctgcgcgcc ctggtgcact tcgacgcccg cgcggccctg cccggcctga cgatgccggc
    23341 cctggcggtg gccggccggc acgacagcgt gtccacgccg cgcgtggtgc gccagatggc
    23401 cgaacgcctg cccgccgcca cctacgccga actccccgcc ggccacctcg cccccttcga
    23461 ggcgccggag gccttcgccc gcctcgtgcg ggacttcctc acccgcctgc ccgaccggcc
    23521 ggcctgacct tccaggatcc gctcccccat ggatgaaagc cccttcaaag gcaagaccgg
    23581 cctgagccgg gtgtggaacg ccttccgcta ctccatggcc ggcctcgccg ccgcctaccg
    23641 caacgaggat gccttccgcc aggaggtgtg gctctccgcc ctgctgatcc ccgtcgccct
    23701 ggtcctgccc gccagcgccg tgggcaaggc cctgatggtg gccagcgtgc tgctggtggt
    23761 gatcgtggaa ctgatcaact ccgccgtgga ggcggcggtg gaccgggtct ccctggagcg
    23821 ccaccacctc gccaagcggg ccaaggacat cggcagcgcg gcggtcttcg tcgccctggt
    23881 gaatgtgatc gccgtgtggg gcctggtcct cgccggctga ggcgggtcgg cgcaccgcgc
    23941 ctccgccgtc tgcctcgcag ggcggagccg ccatcgcccc gttaagggtc gcttaatctg
    24001 gcggggctaa ggtggcgccc atcatcgcgt cgctccgagt ccgcccatgc tccgccccct
    24061 tcgttacctc ccccacggca tcgccgccgc ctgtgccctg atcgccgcct gcgcggccct
    24121 cgccgccgag cccttggtcc tccagccggt ccagctcaag gccctcggca tcgagaccgc
    24181 cgtcgccgcc gaagccgccg ccggccgtgg cgcgcgcctg cccgcccggg tgagcgtacc
    24241 caacgcccag ctgcgggtcg tctccgcgcc ggtggaaggc ctcgtcgaga gcctcgccgt
    24301 ggcgcccggc agcacggtgc gcaagggcca ggaggtggcc cgcctggcca gccgcgccgc
    24361 acttgaattg caacgggacg ccctccaggc cgacagccag gcggccctcc tcaagcacag
    24421 cctgagccgc gacgagaccc tgttccgcga aggcctgatc cccgaatccc gcctccaggc
    24481 cacccgcgcc gccgccgccc aggcgggcgc ccaggccgca gagcgccgtc aggccctggg
    24541 cc
//
LOCUS       DAOQPA010000042        24393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_975_length_24393_cov_17.541006, whole genome shotgun sequence.
ACCESSION   DAOQPA010000042 DAOQPA010000000
VERSION     DAOQPA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24393
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_24393_cov_17.541006"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKX01_07835"
     CDS             <1..776
                     /locus_tag="PKX01_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76072.1"
                     /translation="PPTLGEGVQADDADLCGALEDLSPAPGAPLPDKVFFDPSDYLIG
                     TLAQAVRTTAELGRPQAIAGLPRIIRIEPKPAPTCIAGYRDNQLRPLSMAQLPQSTAR
                     IVPAPSFREHISGEVACAAEELLWNVAAWGARGRIPAGHDPYRPVRLRAWPNFPAIFV
                     SPHALRIAALWVRSHATPVEIASRLAIPQRYVFSFYSAAHFSGLLDLQPPPPTPAPVE
                     SAPQTERVPDTPVASRHPAPSPQKPSLLSRILRKLLNAV"
     gene            766..1326
                     /locus_tag="PKX01_07840"
     CDS             766..1326
                     /locus_tag="PKX01_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HMZ76073.1"
                     /translation="MRSERARHNKIVFSGTVGAGKSTAIAALSDIPPVSTEADATDET
                     ADIKKTTTVAMDYGLLSLPDGEKVMLYGTPGQERFSFMWEILSEGGIGLILLINNDTP
                     APLDDLERYLKAFKNFIASTAVAIGVTHMDKAPVPSLDTYRAKLAEVGLHPMPAVFRV
                     DARSREDVATLVKALLFTLNPGLIDA"
     gene            1319..1951
                     /locus_tag="PKX01_07845"
     CDS             1319..1951
                     /locus_tag="PKX01_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76074.1"
                     /translation="MLEIIPGSYLAVTPYGAWRAVSRADDSIERRILQAILCEAQSPM
                     LSAEAACAWTGESNAEAALGHLLRLQEDASLEAWTTPRKVPFGSMEASLPPLLATLSD
                     TRRAVLADRMGFCLAMAGFPQEQGDALAALGADILSVAERQSAALAQIGDQLLDGWGN
                     VDAAGVSGLSFWPMHVGRIRLALTVSGRPRFNQATFSDLVWSLCVRYGRI"
     gene            3150..3545
                     /locus_tag="PKX01_07850"
     CDS             3150..3545
                     /locus_tag="PKX01_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HMZ76075.1"
                     /translation="MSQLEDLKSELNKIDGEIAALSQRRIDLQTQIDAEQKKYAEEAL
                     SKIVGELKSLNLDPADIAKALGVPVTPTAKKTRAPRGSAPVKSKGEPKYRNSVDPSKT
                     WSGKGRKPEWVITYLANGGKLEDLLIHKA"
     gene            3737..4216
                     /gene="moaC"
                     /locus_tag="PKX01_07855"
     CDS             3737..4216
                     /gene="moaC"
                     /locus_tag="PKX01_07855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HMZ76076.1"
                     /translation="MSDPFTHFDAQGQAHMVDVADKAETRRVARAEGCIVMRPDTLAR
                     ILAGDNRKGDVIGIARIAAIQGAKRTADLIPLCHPIPLTRVSADFTVEGADTIRCTVT
                     AETVGRTGVEMEALTAVQIGLLTIYDMCKAVDRGMLMQGIRLLEKEGGKSGHWVAEA"
     gene            4523..6028
                     /locus_tag="PKX01_07860"
     CDS             4523..6028
                     /locus_tag="PKX01_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HMZ76077.1"
                     /translation="MSHEIELKLTLPKKALAALRKHPLLAEAPREGRTCTLENTYYDT
                     PELELRERRMAVRTRKAGSRWLQTVKCAAESVGGLSARPEWEQPYVNASFDFSTVDRD
                     DVRERLETYHPRLVPVFTTQFRRETVVLTPRPGTRVLAMIDSGTIEAGERSAALCELE
                     LELVDGDPRDLYAIALQLAETLPLTPENVSKAERGYRLYSNLALRPAKARPSRLSAEL
                     SPVDAFRLIAFDCLALWQANESGARESDDPEFIHQLRVALRRLRSALRLFGPALPDDF
                     TRRWSDGLKEAADGLGAARDLDVMLAEVLSPVAESGLADEATQRLIAMAVDRRDREKT
                     AARAQLLAASHGRQMLALAADLYALESDSLVKSAQLSTFARLQLSQLRKRARKRYSAA
                     GDLQAEKLHELRVALKRLRYGIEFLLPLLPQKATVRYANALADAQETLGYLNDEAVAR
                     RRLRDWAGKDPELRSAAAFVAGWHAPRIARLRRNVLPGLEGILWGKAPWSR"
     gene            6101..6553
                     /locus_tag="PKX01_07865"
     CDS             6101..6553
                     /locus_tag="PKX01_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ76078.1"
                     /translation="MDLLLWRHAEAVDGVPDSTRELSERGLRQAHKMAEWLDKRRPKR
                     LRVLVSPTLRTRQTASAFTHDFEVVPALGPGNDIASILAATGWPDAGGAVLIVGHQPE
                     LGRLAALLLAGQEADWTIKKGALWWFTNRVRDEETQTVLRAVVPADLA"
     gene            complement(6613..7059)
                     /locus_tag="PKX01_07870"
     CDS             complement(6613..7059)
                     /locus_tag="PKX01_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HMZ76079.1"
                     /translation="MSDQSKQFEEFYGYTFEELSVGQSAVYARTVTEADILMFAGVSG
                     DYNPVHINQELAESTMFGGRIAHGMLSAAFISTVFGMKLPGPGAIYLSQSLKFKAPVK
                     IGDTVVTRVTIKELVPEKRKAKFDTVCSVRGKNVVEGEAEIMVPAR"
     gene            7253..8101
                     /locus_tag="PKX01_07875"
     CDS             7253..8101
                     /locus_tag="PKX01_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ76080.1"
                     /translation="MSQPDQAVLDRLLRGIDIPPCPAVLTALQREVAAPNASLTRIAQ
                     IINGDVSLAASVLKAANSPAFGLSRRVGNVAQAVPVLGMRTLSAITAGVALRTALSGG
                     ARRLERFWDSSSKTALLCARLTREFTGVPAETAYTFGLFHDCGIPVLIHRFPDYLDTL
                     QLANGSFDLSFTEVEERRHATHHAAIGTLLTRNWGLPEDISTAILLHHDLDIFRPDAD
                     HIAARARTLVALGALASYFVGSSLRMSEDPWWIRGRPLYLAHLGLGDKDFLDLRETAF
                     EAIESF"
     gene            8115..9617
                     /locus_tag="PKX01_07880"
     CDS             8115..9617
                     /locus_tag="PKX01_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMZ76081.1"
                     /translation="MPAWPLYPVSALRAVEALALPGARPPLMERAGAAAADLARRLLG
                     SSAGDVLVLCGPGNNGGDGFVLARHLLQAGHRVCVVCTADPARLPADAAAARQTWLDA
                     GGTTQTHFPAAPASGWALAVDALFGIGLQRPPTGQYAAWIEAFNALDCPRLALDIPSG
                     LDADSGRRLEVCVDATHTVTFIAAKPGVCTLDGPDTCGVLEIAPLGLDPEALVPAPGR
                     TISPALFAECLRPRRRNTHKGSFGDAALIGGAPGMVGAALLAGRAAAFLGAGRVLVGL
                     LAADALAVDPLRPELMFRNAHGLLAENAGAYAIGPGLGQSDAARSITADALRLQRPLV
                     LDADALNLVARHAELQHALTARDAPTLLTPHPAEAARLLACSTADIQTDRIAQALALA
                     RRFNALVVLKGCGSVVAAPDGAWWINTSGNPAMATAGMGDVLTGLIVALLAQGWPARS
                     ALLAGVHLHGCAADLCCAEQGLHAGLLAGEILEPARRCLNRWLGAPEARP"
     gene            9614..11596
                     /locus_tag="PKX01_07885"
     CDS             9614..11596
                     /locus_tag="PKX01_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ76082.1"
                     /translation="MNGTAFDPAGRSIRSIRLFPLPPVDATMPLTDALRVLLDQNVPA
                     ALVRLQGTVIGILSERSAVGACLGADASRLTARDACLLNPPVCGPDTELREAYRLLVR
                     HDSRDLLVCEADGRPTGLVAESDILSALGMEHYAHLDQVGQVMSLRPRSVAETASTED
                     ALRVMRRHDIGSVVVVTDQWPVGIVTFHDLSPRVARGERLHELPVTDVMVRDPITIAP
                     HTSVWEASQIMHRARIRHLPVVHHDRLVGMLAEHDIVTSLEGRYVAFLREIIVEQERE
                     LIAQQVHIARRESEESLRRIIDASPDMVIVKDPQGCWQIANRAALELFGLDHDACHGR
                     AEEAFADRVPQPRREALLRYAAASRHAWQSPDGLRHSDRVPAAEGADVHVEFIEKPMF
                     GADGAPSMLVTLGRDITDRVRAEDAVRALNASLEERVAQRTRELRATLAELETFNYSI
                     SHDLSTPLRAIDGFSGIVMQDYADQLDPQALEYLRRVRDASRRMARMIDDLLDLSRHS
                     RKPLERSPLDLSAMARSVADELVHGDPGRQAHIHVEPGIEAEGDPGLLRTVLQNLLGN
                     AWKFTRPQACADIRFSMRRRQGERIFTVSDNGVGFDAAHAAHLFVPFQRYHAEEDFEG
                     SGIGLSTVQRIISRHGGRIWAESAPGQGCSFHFTLG"
     gene            11600..12472
                     /locus_tag="PKX01_07890"
     CDS             11600..12472
                     /locus_tag="PKX01_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ76083.1"
                     /translation="MHAPPRPARGVLFAMVSLVLFVSLDVTSKYLSQRYPVPLLIWAR
                     YTVHCLLMVLVLGPSQRGRLLATGRPALHVLRAGLLLATSLCGLSAFQRLPLAEATAI
                     AFCSPLIVTLLARPLLGERVGPARWVAVLVGFAGVMLVARPGSGLVPDGVLFAFATAV
                     VYALYQLLTRKMAATERPLTLLFYSALVGSAVMSATLPWLDRGPTPSLPELPFFLAMG
                     TLAGLGHFCLTRAFREAPASLLSPLLYGQLFWATLFGWAVFGQFPDRLALTGMLAIGA
                     SGVLIAVDSRRGMR"
     gene            complement(12482..13318)
                     /locus_tag="PKX01_07895"
     CDS             complement(12482..13318)
                     /locus_tag="PKX01_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ76084.1"
                     /translation="MHAFRFRAGLCCIVGALCLGRAVSAEAALLDEDELAEVVRKPTI
                     GLYKGYAEFKMAHYAEARQIWTALADRGQGEAWFNLGILAEDGLGEPRDGTEARRRYE
                     RAAQAGSRNAALRLGTLLQTARLGAPDPAAARRWLQQAADLGDDEARAQLAALEGRAV
                     AVNGADRRLAEARRLEADGKLADAVDAYRAIAAAGDVRGTTRLAWLFEAGRGVPRDLA
                     EAARLFRQAAEAGEAEAQYALAVMLETGAGQPRDPEEALRWLRASAAGRYPPAVDALK
                     AR"
     gene            13544..14392
                     /gene="nadC"
                     /locus_tag="PKX01_07900"
     CDS             13544..14392
                     /gene="nadC"
                     /locus_tag="PKX01_07900"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMZ76085.1"
                     /translation="MEFADQLRVEVQRSVAASLAEDIGTGDLTARLIPAAREARGRVI
                     TREDAVLCGTAWFDAAFAALEPSASVIWHARDGERVKPGQLLCEVDASARALLTAERT
                     ALNFVQLLSGTATLTATFVEAVAGTRARIVDTRKTLPGLRLAQKYAVKVGGGTNHRIG
                     LYDGILIKENHIIAAGSIRAVIEQARAIAPSNVFIEVEVETLDQLREALAAGARMILL
                     DNMDLDQMREAVRLNEGRAELEASGGVNLQRVRAIAETGVDRISIGSLTKDVRAIDLS
                     LRHIEE"
     gene            14448..15344
                     /locus_tag="PKX01_07905"
     CDS             14448..15344
                     /locus_tag="PKX01_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76086.1"
                     /translation="MRIENDINVAPSESPTVADAVEKLVLRITARDTGIDLSAVTRIL
                     ITADLPDATQRFGGTPVTPDQLTRIVREGQGYMLLFSGVRMLEMFKGGVAEVSRVIHH
                     LHKDLWHLHDVARPRTPKPTTPLARALAPICAIMWQEYRVNRRTAWSVPRDSDLLMHH
                     LAALLQALPGGMQEELTLYFADQDLDALFAKSLGRISHLMQAVAHVQGYLDGIGQPLQ
                     TISPDMHEVVNQSFLAPLWTTTARHLAAGYEHYPAEVADLPLRRDIQNAFATFGLTIR
                     ENDAGDDVWLQVEPPVPPEKMH"
     gene            complement(15373..15726)
                     /locus_tag="PKX01_07910"
     CDS             complement(15373..15726)
                     /locus_tag="PKX01_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMZ76087.1"
                     /translation="MQKILIIVHAPPYGSERCLSALRLAIALAGSDAQPKVELFLMSD
                     ATVMALPHQQDANGNGLQGMVEELVRNGVAIRLCRTCALARGLGDLPLIPGTTIGTLP
                     ELAQATLDADKVITF"
     gene            complement(15803..16009)
                     /locus_tag="PKX01_07915"
     CDS             complement(15803..16009)
                     /locus_tag="PKX01_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HMZ76088.1"
                     /translation="MESRLTEIEIKLALTEDLVETLNLTVHRQQEQIDALQRQLLALA
                     KQVHSTSASTAEPHSLRDEIPPHY"
     gene            16378..17376
                     /locus_tag="PKX01_07920"
     CDS             16378..17376
                     /locus_tag="PKX01_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ76089.1"
                     /translation="MESIGTWWMWAGFFALVLVMLAIDLFAVGGGRRHRVSLREAGLW
                     SAIWVGVTLAFAGALWVWLDGSAGRETATTRTLEFLTGYLIEKSLAVDNVFVWLMLFS
                     FFGIPLELQKRVLTYGVLGALVLRTLMILGGAWLIVQFHWLLYVFGAFLLLTGVKMLW
                     LADARPDLAANPVLRWIRRHVPVTEALHGERFIVTLPGTNGPVRALTPLALALVMVEL
                     SDVIFAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLIAGMADRFALLKYGLALV
                     LVFIGAKMLLLDVVKIPIALSLTVVALLIGGAVMLSLHRERRRALPEPRRDDGGGR"
     gene            17436..19370
                     /locus_tag="PKX01_07925"
     CDS             17436..19370
                     /locus_tag="PKX01_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HMZ76090.1"
                     /translation="MIPTRQHLELLAPAKNADFGIEAINHGADAVYIGGPAFGARAQA
                     GNEVADIERLARHAHRYGARVLVALNTILHDAELDGAEALIRQVYEAGADALIVQDMG
                     LLELDLPPIQLHASTQTDIRHPEKARFLQDVGFSQIVLAREMSLTEIRKVAAVTDCAL
                     EFFVHGALCVAFSGQCYISHAHTGRSANRGECSQACRLPYDLEDQDGHLVTGNRHLLS
                     MKDNDQSANLRALADAGISSFKIEGRYKDIGYVKNVTAHYRNLLDQLLEDAPDYRRSS
                     SGRCTFLFTPQPEKTFNRGATDYFVNGRQGDIGAFDSPKHVGEPVGSILRIDPQRRSF
                     ELSSAVELHNGDGLSFYDRKGELIGLRINRAEKLGDDFRVHTADAVPADLRPGSGVFR
                     NHDHAFERLMDKKTAERRIAVDLHLTDDTDGLVLQVTDEDGFSAQARLPHPLEAAQNA
                     ERARSALHDGLAKLGATIFSARSIRLDLATPWFVPAAALNGLRRDAIEHLEAARLAGL
                     PRPPRRAAVEPPAPYPQTELSYLANVLNERARSFYRRHGVTLIEDAYEANQVLDDASL
                     MITKHCLRYSFNLCPKEVKGIRPDPMTLVNGKERLTLRFDCKRCEMHVIGRLRPAVLD
                     AVPQPVRFHAQAPAALRAGR"
     gene            19395..20105
                     /locus_tag="PKX01_07930"
     CDS             19395..20105
                     /locus_tag="PKX01_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76091.1"
                     /translation="MKLAITLIVLALLIIVGPAAWQFTHTNPAAVPTSGLPWQIDTPA
                     PGESRVFSLTLGRSTLADARTRFGPDAQLAIVATPSEAGNVELYYESITAGFIAGRLI
                     VTADLPAATVEGLRERAAKTEYMQSTTRKATLAEADLPVALAAPIRAIAFIPSANLDE
                     AIVVERFGAPAERLRSNDHIEHLLYPAKGLDVVLDSKGKELLQYVAPARFDALRTPLR
                     AAADQAATAPPAAAPDKP"
     gene            20156..20473
                     /locus_tag="PKX01_07935"
     CDS             20156..20473
                     /locus_tag="PKX01_07935"
                     /inference="COORDINATES: protein motif:HMM:NF039672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HMZ76092.1"
                     /translation="MSTPSTVRHFVTYSGVSLPLKLTTPLEGADLAHRITFYRAHYDA
                     AERLALVEKVVYGEIESTHHYTYYPSGVLQEARVCQIADEETTLMAYDETGALVSTTR
                     LED"
     gene            20485..20745
                     /locus_tag="PKX01_07940"
     CDS             20485..20745
                     /locus_tag="PKX01_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76093.1"
                     /translation="MAVQSVQRRRCLTCNRWGGQRRPGDEPDTVEFDEAHDRGPCQDG
                     PWHGTLRGPRNACGQWLRWTLIVPAPDPAEAPAQASPPHEQP"
     gene            20732..21124
                     /locus_tag="PKX01_07945"
     CDS             20732..21124
                     /locus_tag="PKX01_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ76094.1"
                     /translation="MNSPDDSDLLVACLCAAWCGTCRDYAAGFHALAERFPGARFLWL
                     DVEDEADLLDDYDVENFPTLLIQRGDAVLFFGTMLPHHELLERTVRAFASMTPDESRH
                     YAQADATRRTWQHDRNLRRALAERDGDA"
     gene            21149..21559
                     /locus_tag="PKX01_07950"
     CDS             21149..21559
                     /locus_tag="PKX01_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HMZ76095.1"
                     /translation="MDTCVFCRIVRGELPASVIHEDEHTLAFMNIMAGNPGHALVIVK
                     PHVENIYTLDDALAAAVFRTTTRVAKAVRAAAGCEGVSLFQANEVAGGQTVFHFHIHV
                     LPRLPGDALPGVWPAVAPSRDALDAMAARLRAAL"
     gene            21694..23214
                     /locus_tag="PKX01_07955"
     CDS             21694..23214
                     /locus_tag="PKX01_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ76096.1"
                     /translation="MHIPLLSAEAPITLILMVIAGLIMLRPLAHLRRSLMNTGVFYGA
                     CFAGDLLAGLLEGSSQPAAARWLRELCVFGEGLAVVRFVGLTLFQVILPRLHVNISGI
                     LADILVIVGYILWGFIRLSMSGVEFTALFTTSAVLTAVVAISMQETLGNLLGGLALQM
                     DNSLEIGDWIQMGDLAGRVTDIQWRYTAIVTRAGERMVVPNSHLMKNRYAIVCDKRQS
                     NPHQRRHIPFNVDLSVPPARVIGAVLQDILTARIPNVSATPAPACLLMDFGPGFARYQ
                     LRYWLDDPGPDEITDSDVRSHILAALQREDIRLAVADQTVHLVQEGKAHRAEVQAREL
                     NRRLQAITHIDLFCKLSEAEQLELARRLVNAPFARGDIVTRQGAVAHWLYIIVSGEAE
                     AWWQPPDGSPQRLLEKRGPGTVFGELGLMTGAPRRATVIATTDVEAYRLDKTGFEHII
                     RARPEIAEGISAILERRLSHFQALERGDGAPRPDGNKPANGDTGVIGRKIREFFGL"
     gene            complement(23313..24023)
                     /locus_tag="PKX01_07960"
     CDS             complement(23313..24023)
                     /locus_tag="PKX01_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76097.1"
                     /translation="MKTNRRSVSGPALAVLALTLGACASPRENAIFVTKTSLSVFDAE
                     TVPAGVSIAYDRVEGYAGPRFDNGKVFPVVGVIETEGQGILREIRQMYATGRAAELMT
                     AGTAAPPAATVAAGPSPGSPPVLFFATSTVLGLKLAFQASNYAPESFTFGYKRKEASV
                     IPVDQTRQPSVFASLNTSAGVSAPAATTGSSGATAKFQLSQFFATGTAAEQLAANRTV
                     SGELEEKAKKAFTPSPAQ"
     gene            complement(24028..>24393)
                     /locus_tag="PKX01_07965"
     CDS             complement(24028..>24393)
                     /locus_tag="PKX01_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76098.1"
                     /translation="SMAAWRAVGRWIPAALRGLGCALLVLPAACSYLYVAPDGTRHVV
                     GFVHLEIPPGNPAPWGGESLRVRSLGVSVSSSHESHALVLGYSDATHVAVRENACFAP
                     EMAVSAEPVSPVEAPTVKE"
BASE COUNT         3942 a         9720 c         7056 g         3675 t
ORIGIN      
        1 ccccccccac cctgggtgaa ggcgttcagg cggacgacgc ggacctgtgt ggcgcactcg
       61 aagatctttc acccgccccg ggcgccccgc tgccggacaa ggttttcttc gatccctccg
      121 actacctgat cgggaccctt gcccaggcgg tcaggaccac cgccgagctc ggtcggcccc
      181 aggccatcgc cggactgccg cggatcatcc gcatcgaacc gaagcccgcc cccacctgca
      241 tcgccggcta ccgggacaat cagctccggc ccctgtccat ggcccaactc ccccaaagca
      301 cggcccgtat cgtgccggcc ccgtcgttcc gggaacacat ctccggtgag gtcgcctgcg
      361 ccgcggaaga gctcctctgg aatgtggccg cctggggcgc ccgcggacgg attcccgcag
      421 gccacgaccc ctaccgcccg gtccgactcc gggcctggcc gaatttcccc gccatcttcg
      481 tgtcccccca cgccctgcgt atcgctgccc tgtgggttcg cagccatgcg accccggtcg
      541 agatcgccag ccggctcgcg attccgcagc gttatgtctt ctcgttctac agtgcggcac
      601 atttttcggg cctcctggac cttcagccac caccgcccac gccggctccc gtagaatccg
      661 cgccgcagac ggaacgcgtg ccggatacac cggtcgcgtc gcgacatcca gccccgtcgc
      721 cccagaaacc gtcactgctt tcgcgaatcc tgaggaaact cctgaatgcg gtctgaacgc
      781 gcccgccaca acaagatcgt cttctcgggc accgtcggtg caggcaagag cacggccatc
      841 gccgccctca gcgacatccc gccggtgagc accgaagccg acgcaaccga cgagacggcc
      901 gacatcaaga agaccaccac agtcgccatg gattacgggc tcctgagcct gcccgacggc
      961 gaaaaggtca tgctctacgg cacccccggc caggagcgct tcagcttcat gtgggaaatc
     1021 ctgtcggaag gcggcatcgg cctgatcctc ctgatcaaca acgacacccc cgcccccctg
     1081 gacgacctgg agcgctacct caaggccttc aagaacttca tcgcgtccac tgccgttgcc
     1141 attggcgtca cccacatgga caaggccccc gtacccagcc ttgacaccta ccgggccaag
     1201 ctcgccgaag tcggcctcca ccccatgccg gccgtcttcc gcgtcgatgc ccgcagccgg
     1261 gaagacgtgg cgacgctcgt caaggccctc ctcttcaccc tgaacccggg tctgatcgat
     1321 gcttgagata atccccggct cctacctcgc cgtgacgccc tacggcgcct ggcgggctgt
     1381 gtcccgtgcc gacgactcca tcgaacggcg cattctgcag gccatcctgt gcgaggccca
     1441 gtccccgatg ctgagcgccg aggccgcctg cgcgtggacc ggcgagagca acgccgaagc
     1501 cgcgctcggt catctcctgc gcctgcagga ggacgcgagc ctggaagcct ggaccacgcc
     1561 gcgtaaagtg ccgtttggct ccatggaggc cagcctgccg cccctcctcg ccaccctcag
     1621 cgatacccgt cgggccgtgc tggccgaccg catgggcttc tgcctcgcca tggccggttt
     1681 cccccaggag caaggcgacg cccttgcggc cctcggcgcc gacatcctgt cggtggccga
     1741 acgccagagc gctgccctgg cccagatcgg cgaccagctt ctggacggct ggggcaacgt
     1801 ggacgccgcc ggagtcagtg gattgtcctt ctggcccatg cacgtgggac gcatccgtct
     1861 cgccctcacc gtgagcggcc gtccccgctt caatcaggcc acgttctcgg acctcgtttg
     1921 gtccctgtgc gtccgctacg gccgcatctg acaccactcg gtcccacgcc caccggatgc
     1981 cgcgtacccg cgatcacaca atcgccgcag gactctgact ccgtcgcacc cgccgggacg
     2041 gccgacgcga caccgtctgt gcgcgcgcac gccgaattca tccccaccgc agtagctcag
     2101 acgggcccgc acgtagccgg cgtcgcctac caaccgacgc cgcaccgcct acgcacgttc
     2161 gcacgttgca ccgccggcgg ggccgcagca taccgaatgc cggcatcgca attgtccaac
     2221 gaaatcgcgg aatgatcgga tcgtccaggc aaagtcgtgc caatgaccct aaaagggatc
     2281 cgctcaaaac gacggtaaac gagaaatatg aaccgcaagg cggcattcga gcctggcctg
     2341 tcgaatcgga atgcacggca tgcaatctca tcgacgcgga ttgcccccgc caccacgagg
     2401 attgccgcgc cccgtatcca tcgattaccg aatgcccagc gccgggctga ctgccatccg
     2461 atgcaagacc atgaaaatgt gcgcaattca cctgatgcct cgcacaaccg ccacggcgat
     2521 caccgaccgg ctccgggcat ttaaaactcg cacgtgccga atggcggacc accgcgtcac
     2581 cttgcattgc ggcctcgccg tgtggcttta gcgagcccct tgcccgcaga ggcaccgtcg
     2641 tcgaaatccg atcgcgccgt agccgctgaa cagatcactc aggcaagcac tccccgatac
     2701 acgggctgcc gcgccggaca gtggccccca tgccacctgc gcacatcgcg agccgatgac
     2761 tgcgcgaatc cagcgcagga aatcagcggg tcatgaccga tcgcccgcga agcacccgac
     2821 ccgacggaaa cccatgaacg gcaagggttt tcaattggcg gcacgaactt tccggagagc
     2881 gcagcaatca aagtcagctt catgcaagac tttaacgctg ccacaaagca tatattcccc
     2941 acccgctcac ccggcgccgc accgattcca ataggcgctg ttgcctgcaa caatcaggca
     3001 tccatacggg tctcgaatcg ccttaatgtt gtgtcaaagg ccttgcccac gccaatattc
     3061 accattttac gctccaacac ctattcagct attgcaccac tattgcttta gtgatacgtt
     3121 tcgcccactc tcaacacgac aggtgcctta tgagccagct ggaagacctg aaaagcgaac
     3181 tgaacaagat cgatggcgaa attgccgctt tgtcgcaacg tcgcatcgac ctgcagacgc
     3241 aaatcgatgc cgaacagaag aaatatgccg aagaagccct cagcaagatt gtgggcgaac
     3301 tcaagtcgct gaatctcgac ccggccgaca ttgcgaaggc cctcggcgtt ccggtcacgc
     3361 cgaccgccaa gaagacgcgt gcgccgcgcg gttcggctcc cgtcaagagc aagggcgaac
     3421 cgaagtatcg caacagcgtc gatccttcca aaacgtggag cggcaagggc cgcaagcccg
     3481 aatgggtgat cacctatctg gccaatggcg gcaagcttga agacctgctg atccacaagg
     3541 cctgagcgca tcccggcctt tcgccgggac tgccccagca gtccttcaca gcccgcattc
     3601 cgctgcgcag gtagccggtg cgggctgttt gcatgacggc aacgccccga cggttttgcg
     3661 tcggcaccgc cgcattccac ccttgcgtgt catccgcgga ttccgcgatt ccatcggttc
     3721 aacgacccca tctgccatga gcgacccctt cacccacttc gacgcccagg gccaggccca
     3781 tatggtggac gtcgccgaca aagccgaaac ccgccgtgtc gcccgcgccg aaggctgcat
     3841 cgtcatgcgc cccgacaccc ttgcacgcat cctcgctggc gacaatcgca agggcgacgt
     3901 gatcggcatc gcccgcattg cagccatcca gggcgccaag cgcaccgccg acctcattcc
     3961 cctgtgccac cccatccccc tcacgcgggt gagcgctgac ttcacggtcg aaggcgcgga
     4021 caccatccgc tgtaccgtca ccgcagaaac cgtggggcga accggtgtcg aaatggaggc
     4081 gctcacggcc gtgcagatcg gcctcctgac catctacgac atgtgcaagg ccgtggaccg
     4141 gggcatgctc atgcaaggca tccgcctcct cgaaaaggaa ggtggcaaat ccgggcactg
     4201 ggtggccgaa gcctgagcgc cgaccggctc ccgaccctgc accgcgaggc cggatttcgg
     4261 ccggcacgcg gacccgcgtg ggctggcgtc ggcgggcccc cgcatcgccg ccgggccgat
     4321 gtccggatcg acctggtcaa tcgggcgccg tgccgccatc acccgccccg atcgggcggc
     4381 agttgcgtcc tgccctgccc gcccataggg ctccgtgccg tacgccccaa aacagggcat
     4441 gcgcaagaca ggggtcaagc ttcgggtcta gaatgggcgc cgccggaacg ccctgccggt
     4501 gcctcccctg atagatggca tcatgagcca cgaaatcgaa ctgaagctga ccctccccaa
     4561 aaaggcgctc gcggccctgc gcaagcaccc cctcctggcc gaagcccccc gcgaaggccg
     4621 cacctgcacc ctcgaaaaca cctactacga cacgccggag ctggagctgc gcgagcggcg
     4681 catggccgtg cgcacccgca aggccggcag tcgctggctg cagaccgtca aatgcgcggc
     4741 cgaatccgtc ggtggcctga gtgcccgccc ggaatgggaa cagccctacg ttaacgccag
     4801 cttcgacttt tctaccgtgg accgggacga cgtgcgcgag cgcctcgaaa cctaccaccc
     4861 gcgcctcgtc cccgtcttca ccacccagtt ccggcgcgag accgtagtgc tcacgccacg
     4921 ccccggcacc cgcgtgctgg cgatgatcga cagcggcacc atcgaagccg gcgagcgcag
     4981 cgcagcgctg tgcgaactgg aactggaact cgtcgacggc gacccccgcg acctctacgc
     5041 catcgccctg caactcgccg agaccctgcc cctcacgccc gagaacgtca gcaaggccga
     5101 gcgcggctac cgcctgtact ccaacctcgc cctgaggcct gccaaagccc ggccgtcgcg
     5161 cctcagcgcc gagctctcgc cggtggacgc cttccgcctg atcgccttcg actgcctggc
     5221 cctgtggcag gccaacgaat ccggcgcccg cgaaagcgac gacccggaat tcatccacca
     5281 gctccgcgtc gccctgcgcc gcctgcgctc tgccctgcgc ctgttcggac cggccctgcc
     5341 cgatgacttc acacgacgct ggtccgacgg cctcaaggag gccgccgacg gcctgggtgc
     5401 cgcccgcgac ctggacgtga tgctcgccga agtgctcagc ccggtggccg aatccggcct
     5461 cgccgacgaa gccacccaac gcctcatcgc catggccgtg gatcgtcgcg accgggaaaa
     5521 aaccgccgcc cgcgcccagc tcctcgccgc cagccacggt cgccagatgc tcgccctcgc
     5581 ggcagatctg tatgccctgg agagcgattc cctggtcaaa tccgcccagc tctccacttt
     5641 cgcccgcctg cagctgagcc agctgcgcaa gcgcgcccgc aagcgctaca gcgccgccgg
     5701 cgacctacag gccgaaaagc tccatgaact gcgcgtggcc ctcaagcgcc tgcgctacgg
     5761 catcgaattc ctgctcccgc tgctgcccca gaaggccacc gtgcgctacg ccaacgccct
     5821 cgccgacgcc caggagaccc tcggctacct caacgacgaa gccgtcgccc gccgccgcct
     5881 gcgggactgg gccggcaagg atccggaact gcgcagcgcc gcggcattcg tggccggctg
     5941 gcatgccccg cggatcgccc gcctgcgtcg caacgtgctg cccggcctgg aaggcatcct
     6001 gtggggcaag gccccgtgga gccgttgaag cgtgtcatcg tccggtaaca ccccaccccc
     6061 agactgcccg cctctttggg gagaagagga gacacctacc atggatctgc tgctgtggcg
     6121 tcacgccgaa gccgtggacg gcgttcccga cagcacgcgg gaactcagcg aacggggcct
     6181 gcgccaggcc cataagatgg ccgaatggct ggacaagcgc cggcccaagc gcctgcgcgt
     6241 gctggtcagc cccacgctgc gcacgcgcca gacggcgagc gccttcaccc acgatttcga
     6301 agtcgtcccg gcactcggcc cgggcaacga catcgcctcc atcctcgccg ccaccggctg
     6361 gccggatgcc ggcggtgccg tgctgatcgt cggccaccaa cccgaactgg gccgtctcgc
     6421 cgcgctgctg ctggccggcc aggaggccga ctggacgatc aagaaaggcg cgctgtggtg
     6481 gttcaccaat cgcgtccgcg acgaggagac ccagaccgtc ctgcgcgccg tggtgcctgc
     6541 cgacctggcc tgagccccgc agcacaaaaa aagccgcccg cgggcggctt ttttgttggc
     6601 gcagcgccgg ggtcagcggg ccggcaccat gatctcggct tcgccttcga ccacattctt
     6661 gccgcgcacg gagcacaccg tgtcgaactt cgccttgcgc ttttcgggca ccagttcctt
     6721 gatggtgacg cgggtcacca cggtgtcgcc gatcttcacc ggcgccttga acttgagcga
     6781 ctgggacagg tagatcgcgc ccgggccggg caacttcatg ccgaacaccg tggagatgaa
     6841 ggccgccgac agcatgccgt gggcgatgcg gccgccgaac atggtggatt cggccagttc
     6901 ctggttgatg tgcaccgggt tgtagtcgcc ggacacaccg gcaaacatca ggatatccgc
     6961 ttcggtcacg gtacgcgcgt agacggcgct ctgcccgaca gagagctcct cgaacgtgta
     7021 gccgtagaat tcttcaaact gcttgctctg atcgctcatg gctgttccct taacgtgggt
     7081 tcttggttgt gttcgattct cgatccgtcc cgggcacacc gcccggaacg gatgcttcgc
     7141 cagcataccg caatccgccg tacggcgaag ccccgtttcc atgacagttc cccggggcca
     7201 cgccaagcgc ttgggctaga atctcggtca aagcccgccc ccctaaagca ccatgtccca
     7261 gcccgaccag gccgttctcg accgactcct gcgcggaatc gacattcctc cctgccccgc
     7321 cgtcctcacc gccctgcagc gggaagtggc cgcccccaac gcctccctga cccgcatcgc
     7381 gcagatcatc aatggcgacg tatcccttgc cgcttccgtt ctgaaggcgg ccaactcgcc
     7441 cgccttcggc ctgtcgcgcc gggtgggcaa cgtggcccag gccgtgcccg tgctgggcat
     7501 gcgcaccctg tcggcaatca ccgcgggcgt cgcgctgcgc accgccctgt ccggcggcgc
     7561 ccgccggctc gagcggttct gggactcatc cagcaagacg gccctgctct gcgcccgcct
     7621 gacccgcgag ttcaccggcg taccggccga gaccgcctac accttcgggc tcttccacga
     7681 ttgcggcatc cccgtgctga tccaccgctt cccggattac ctcgacacgc tccagcttgc
     7741 gaacggcagc ttcgacctca gcttcaccga agtcgaggaa cgccgccatg ccacccacca
     7801 cgcggccatc ggcaccctgc tcacccgcaa ctgggggctg cccgaagaca tcagcacggc
     7861 gatcctgctt caccacgacc tggacatctt ccgccccgat gccgaccaca tcgccgcccg
     7921 cgcccgtacc ctggtggccc tgggcgccct cgccagctat ttcgtgggca gttccctgcg
     7981 catgagcgaa gacccctggt ggattcgcgg acgccccctg tatctggcgc acctgggcct
     8041 cggggacaag gacttcctcg acctgcgcga gacggccttc gaggccatcg aatcgttctg
     8101 accggatccc cgccatgccc gcctggccgc tctatcccgt gtccgccctg cgcgccgtcg
     8161 aagccctcgc cctgcccggc gcccggccgc cgctgatgga acgtgccggg gccgccgcag
     8221 ccgacctcgc ccgacggctc ctcggaagct ccgcgggcga cgtgctggtg ctgtgcggac
     8281 cgggcaacaa tgggggcgac ggattcgtgc tcgcccgcca ccttctccag gccgggcacc
     8341 gggtctgcgt cgtctgcacg gcggacccgg cacgcctgcc ggccgacgca gcggcggccc
     8401 ggcagacctg gctcgacgcc ggcggcacca cgcagacgca cttccccgcc gcgcccgcct
     8461 ccggctgggc cctcgccgtg gacgccctct tcggcatcgg cctgcaacgt ccgccgaccg
     8521 ggcaatacgc cgcgtggatc gaggcattca acgcactgga ctgtccccgc ctggccctgg
     8581 acatccccag cgggctcgat gccgacagcg ggcgacgcct ggaggtctgc gtcgacgcca
     8641 cccacaccgt caccttcatc gccgccaaac ccggcgtgtg caccctggat ggccccgaca
     8701 cctgcggcgt actggagatc gcccccctcg gtctcgaccc cgaagccctg gtccccgcac
     8761 cgggccgcac gatctcgccg gcgcttttcg ccgagtgcct gcggccccgc cgtcgcaata
     8821 cccacaaggg cagcttcggt gacgcggcac tgatcggtgg cgcaccgggc atggtcggtg
     8881 ccgcactcct cgccggccgt gccgcggcct ttctgggtgc cggacgggtg ctggtcgggc
     8941 tcctcgcggc cgacgccctg gcagtggacc cgctgcgccc cgaactcatg ttccgtaacg
     9001 cccacggcct gctcgccgaa aacgccggcg cctacgccat cggccccggc ctgggccaat
     9061 ccgacgccgc acggtcgatc acggccgatg ccctgcgcct gcagcgcccc ctggtgctgg
     9121 acgcggacgc cctcaatctc gtggcgcgac acgcggaact gcagcacgcc ctcaccgccc
     9181 gcgacgcgcc caccctcctc accccgcacc ccgccgaggc ggcgcgcctg ctggcctgct
     9241 ccacggccga catccagacc gaccgcatcg cccaggccct cgcccttgcc cgccgattca
     9301 acgcactggt ggtgctcaaa ggttgcggca gcgtcgtcgc cgcgccggac ggtgcctggt
     9361 ggatcaacac cagcggcaac ccggccatgg ccacggccgg catgggcgac gtcctcacgg
     9421 gcctgatcgt cgccctcctc gcccagggct ggcctgcccg ctcggccctg ctggcgggcg
     9481 tccatctcca cggatgtgcg gccgatctgt gctgcgccga gcagggcctg cacgcggggc
     9541 tgctggccgg cgagatcctg gaacccgcgc gccgctgcct caaccgctgg ctgggtgcgc
     9601 cggaagcacg gccatgaacg gtaccgcctt cgaccccgcc ggacgcagca tccgctcgat
     9661 ccgcctcttc ccccttcccc cggtcgacgc gacgatgccg ctcaccgatg ccctgcgcgt
     9721 cctgctcgat cagaacgtac ctgccgccct ggtgcgcctg cagggtacgg tgatcggcat
     9781 cctgtccgag cgcagtgcgg tgggagcgtg tctgggcgcc gacgcatccc gactcaccgc
     9841 ccgggacgcc tgcctgctca atccacctgt ctgcggtccg gacaccgagc tgcgcgaggc
     9901 ctaccgcctc ctcgtccgcc acgacagccg cgatctgctg gtgtgcgaag cggacggacg
     9961 acctaccggg ctggtggccg agagcgacat tctctcggcg ctgggcatgg agcattacgc
    10021 ccacctggac caggtcggcc aggtcatgag cctgcgcccc cgctcggtgg ccgagaccgc
    10081 cagcaccgaa gacgctttgc gtgtcatgcg gcgccacgac atcggcagcg tggtcgtcgt
    10141 gaccgaccag tggccggtcg gcatcgtcac cttccacgac ctcagcccgc gggtggcccg
    10201 cggcgagcgc ctgcacgaac tccccgtcac cgacgtgatg gtgcgcgacc ccatcaccat
    10261 cgccccccac acctccgtgt gggaggcctc ccagatcatg caccgggccc gcatccgcca
    10321 cctgccggtg gtacaccacg accgcctggt gggcatgctc gccgaacacg acatcgtcac
    10381 cagcctggaa ggccgctatg tggcctttct gcgggagatc atcgtcgaac aggaacggga
    10441 actcatcgcc cagcaggtcc acatcgcccg ccgcgagagc gaggagagcc tgcgccgcat
    10501 catcgacgcc tcgcccgaca tggtcatcgt caaggatccg cagggctgct ggcagatcgc
    10561 caaccgggcc gcgctcgaac tcttcggact cgaccacgac gcctgtcatg gccgcgccga
    10621 agaagcgttc gccgaccgcg ttccgcagcc ccgccgggag gccctcctgc gctacgccgc
    10681 cgccagccgc cacgcctggc agtccccgga cggcctgcgc cacagtgacc gcgtgccggc
    10741 cgccgagggc gccgacgtcc acgtggaatt catcgaaaag cccatgttcg gcgccgacgg
    10801 tgccccatcg atgctggtca ccctggggcg cgacatcacc gaccgggtcc gcgccgagga
    10861 tgccgtccgc gccctcaacg cgtccctcga agagcgggtc gcccagcgca cccgcgagct
    10921 tcgcgccacc ctcgccgaac tggagacttt caactactcc atctcccacg acctgagcac
    10981 gcccctgagg gccatcgacg gcttctcggg catcgtgatg caggattacg ccgaccagct
    11041 cgacccccag gccctcgaat acctgcgccg cgtgcgcgac gcctcccggc gcatggcgcg
    11101 catgatcgac gatctgctcg atctctcccg ccactcccgc aaacccctcg aacgcagccc
    11161 gctggacctc tctgccatgg cgcgcagcgt cgccgacgaa ctcgtccacg gcgacccggg
    11221 ccgccaggcg cacattcatg tcgagccggg catcgaggct gaaggcgacc ccggcctgct
    11281 gcgcaccgtg ctgcagaacc tgctgggcaa tgcgtggaag ttcacccgcc cgcaggcctg
    11341 cgccgacatc cgcttcagca tgcgccgccg gcaaggcgag cggatcttca cggtgagcga
    11401 caacggcgtc gggttcgacg ccgcccacgc cgcgcatctc ttcgtaccct tccagcgcta
    11461 ccacgccgag gaggacttcg aggggagcgg gatcggcctg tccaccgtgc agcgcatcat
    11521 cagccgccac ggcggccgga tctgggccga gagcgcgccc ggacagggct gcagcttcca
    11581 cttcaccctc ggctgacgca tgcacgcgcc gccacgcccg gcccggggcg tcctcttcgc
    11641 catggtgtcg ctggtgctct tcgtgtccct ggacgtcacc tccaagtacc tctcccagcg
    11701 ctatcccgtg ccgctgctga tctgggcgcg ctataccgtc cattgcctgc tgatggtgct
    11761 cgtcctcggc cccagccagc gcggccgtct gctcgccacc gggcggcccg ccctgcacgt
    11821 gctgcgtgcc ggcctgctgc tcgccaccag cctgtgcggg ctgtcggcct tccagcgcct
    11881 gcccctcgcc gaagccaccg cgattgcctt ctgctcgccc ctcatcgtca ccttgctggc
    11941 ccgaccgctg ctgggcgagc gggtcggtcc ggcccgctgg gtcgcagtac tggtcggctt
    12001 tgccggcgtg atgctggtgg cgcgcccggg cagtggtctg gtgcccgacg gtgtactctt
    12061 cgccttcgcc accgccgtgg tgtatgccct ctatcagctc ctcacgcgca agatggcggc
    12121 gaccgagcgg cctctcaccc tgctcttcta ctccgccctg gtcggcagcg cggtcatgtc
    12181 cgccaccctg ccctggctag accgtgggcc gacgccgagc ctcccggagc tgcccttctt
    12241 cctcgccatg ggcaccctcg ccgggctcgg ccacttctgc ctgacccgcg ccttccgtga
    12301 agcccccgcg tccctgctgt cgccgctact gtacggccag ctgttctggg cgacgctctt
    12361 cggatgggcc gtgttcggcc agtttccgga tcgcctcgcc ctgaccggca tgctcgccat
    12421 cggcgcgtcg ggcgtactca tcgccgtgga cagccgacgc ggcatgcgct gacggacgcc
    12481 ctcagcgcgc tttcaaggcg tccaccgcgg gtggatatcg ccccgccgcc gacgcccgca
    12541 accagcgcag cgcctcctcg ggatcgcggg gctgcccggc gcccgtctcc agcatcaccg
    12601 ccagcgcgta ctgagcttcc gcctcgcccg cttcggccgc ctgccggaac agccgggccg
    12661 cctccgccag atcccgcggg accccgcgac cggcctcgaa cagccaggcg aggcgcgtcg
    12721 tcccgcggac atcccccgcc gcggcgatgg cgcggtaggc atccacggcg tcggccagtt
    12781 tgccgtccgc ctccagccga cgggcctcgg cgaggcgccg gtcagcccca ttcacggcca
    12841 cggcgcggcc ctccagcgca gccagctggg cccgtgcctc gtcgtctccc agatcggcgg
    12901 cctgctgcaa ccagcgccgc gccgcggccg gatcgggcgc cccgaggcgg gccgtctgca
    12961 gcagggtgcc caggcgcagc gccgcgttgc gactgccggc ctgcgccgcc cgttcataac
    13021 gccggcgcgc ctcggtgccg tcccgcggct cccccagccc gtcctccgca aggatgccca
    13081 ggttgaacca cgcctcaccc tggccccggt ccgccagggc cgtccagatc tgccgggctt
    13141 ccgcgtaatg ggccatcttg aactccgcat agcccttgta gaggccgatg gtcggcttgc
    13201 gcaccacctc ggccagctcg tcctcgtcaa gcagcgcggc ctccgcggaa acggcgcggc
    13261 ccaggcacag cgcgccgacg atgcagcaca ggccggcacg aaatcggaag gcgtgcatgg
    13321 tgagactcct ctgtctgccg tggccgcaac cccatcgcga ccctcgccgg cttggagtac
    13381 ggcgtggggt cccggagttc ccccggtgcc cgccatccgt accggccgtg ccgtgcacgc
    13441 agcggtggcc cggtgcgcgc gccatgcccc cggtctgcgc gagcccgctc cccctccccg
    13501 cccggtgtta gaatcgcggc ctgttttctc tcaggcgact ccgatggaat ttgcagatca
    13561 gcttcgcgtt gaagtccagc gctccgtggc ggcctccctg gccgaagaca tcggcaccgg
    13621 cgacctgacc gcccgcctca tccccgccgc ccgcgaagcc cgtggccgcg tgatcacccg
    13681 cgaagacgcc gtgctgtgcg gcaccgcctg gttcgacgcc gccttcgccg cgctggagcc
    13741 cagcgcctcg gtcatctggc atgcccgcga cggcgagcgg gtcaagcccg gccagctgct
    13801 ctgcgaagtc gacgccagcg cccgcgccct gctcaccgcc gagcgcaccg cgctgaactt
    13861 cgtccagctc ctgtccggca ccgccaccct caccgccacc ttcgtcgaag ccgtggccgg
    13921 cacccgcgcc aggatcgtcg acacccgcaa gaccctgccc ggcctgcgcc tggcgcagaa
    13981 gtacgcggtc aaggtgggcg gcggcaccaa ccaccgcatc ggcctctacg acggcatcct
    14041 catcaaggaa aaccacatca tcgccgccgg cagcatccgc gcggtgatcg agcaggcccg
    14101 cgccatcgcc ccgtccaacg tcttcatcga ggtcgaggtg gaaaccctcg accagctccg
    14161 cgaagccctc gccgccggcg cccgcatgat cctgctggac aacatggacc tggaccagat
    14221 gcgcgaagcc gtgcgcctca acgaaggtcg cgccgagctg gaagcctccg gcggcgtcaa
    14281 tctgcaacgc gtccgcgcca tcgccgaaac cggggtcgac cgcatctcca tcggcagcct
    14341 caccaaggac gtgcgcgcca tcgacctgtc cctgcgtcac atcgaagagt aagcctcacc
    14401 cgccaggccc ggccgagcac cgggtccgcg ccagccctgc gcctgccatg agaatcgaaa
    14461 acgacatcaa tgtcgccccg tccgaaagcc ccaccgtcgc cgacgccgtc gaaaagctcg
    14521 tgctgcgcat cacggcccgc gacaccggca tcgacctgtc ggccgtcacc cgcatcctga
    14581 tcaccgccga cctgcccgac gccacccagc gcttcggcgg cacgccggtg acccccgatc
    14641 agctgacccg catcgtccgc gagggccagg ggtacatgct gcttttcagt ggcgtcagga
    14701 tgctggagat gttcaagggc ggcgtcgccg aagtctcccg cgtgatccat cacctgcaca
    14761 aggacctgtg gcacctccac gacgtggccc ggccgcggac gcccaagccc accaccccgc
    14821 tggcccgcgc cctcgcgccg atctgcgcca tcatgtggca ggaataccgg gtcaaccggc
    14881 gcaccgcctg gtccgtgccg cgggattccg atctgttgat gcaccacctg gccgccctcc
    14941 tccaggcgct gcccggcggc atgcaggaag agctcaccct gtacttcgcc gaccaggacc
    15001 tggacgccct cttcgccaag agcctggggc gcatttccca cctcatgcag gcggtcgccc
    15061 acgtgcaggg ctacctggac ggcatcggcc agccgctgca gaccatcagc cccgacatgc
    15121 acgaggtggt gaaccagagc ttcctcgcgc ccctgtggac gaccaccgcc cgacacctgg
    15181 ccgcgggcta cgagcactat ccggcagaag tggccgacct gcccctgcgc cgcgacatcc
    15241 agaacgcctt cgccaccttc ggcctcacca tccgcgaaaa cgacgcgggc gacgacgtgt
    15301 ggctgcaagt cgaaccgccc gtcccgcccg aaaagatgca ctgagacccc ggcgccgggc
    15361 tgctgccgcg cgtcagaagg tgatgacctt gtccgcgtcc agggtcgcct gggccagctc
    15421 cggcagggtg ccgatggtgg tgccggggat caggggcaga tcaccgagcc cccgcgccag
    15481 ggcgcaggtg cggcacaggc ggatcgccac cccgttgcgc accagctcct ccaccatgcc
    15541 ctgcagcccg tttccgttcg cgtcctgctg atggggcaag gccatcaccg tggcgtccga
    15601 catcaggaag agctccacct tcggctgcgc gtcgctgccc gccagggcga tggccagacg
    15661 cagcgcggac aggcagcgct cgctgccgta gggaggcgca tggacgatga tcagaatctt
    15721 ctgcatggtg atgtccttca aagaaaaggg aatgccgcac tgctcgccgg cccggtggcg
    15781 cacggccgac cggcgggcag catcagtaat ggggcgggat ctcgtcccgc agggaatggg
    15841 gctcggcggt actggccgac gtgctgtgca cctgcttggc gagcgccagc agctgccgct
    15901 gcaaggcgtc aatctgctcc tgctggcgat gcacggtcag attgagggtc tccaccagat
    15961 cttcggtgag agccagcttg atttcgatct cggtcagtcg cgattccatg ggcgtttccg
    16021 ttccggcaga gggccgaccc ggattgttcc agccagcggc gccggcgcca agcccttgcg
    16081 acaccgatcc gatgcacctt gacccgccaa gggtcgtcac ctaagctggg gactgtccca
    16141 agggggagta gctcttccgc cgtggcgtcg tcagtacggg caggtttctc ctgccccggc
    16201 gtcacgggtt gccggcccag tccggcagcg agcaagacct ttgccgcgat ggcgaaggtc
    16261 tcatggcact ccctccccgg atccaacggc ccttcgtcct cgctggacaa agggccgttc
    16321 tttcgtggca tgccgccacg gaacggcgga tgccatccgg acctggagac agcactcatg
    16381 gaatcaatcg gaacctggtg gatgtgggcc ggcttcttcg ccctcgtcct ggtcatgctc
    16441 gccatcgacc tctttgccgt gggcggcgga cgccgccatc gggtgtccct gcgggaagcc
    16501 ggcctctggt cggccatctg ggtcggcgtc accctcgcct ttgccggcgc cctgtgggtc
    16561 tggctggacg gcagcgccgg gcgcgagacg gccaccacgc gcacgctgga attcctcacc
    16621 ggctatctca tcgaaaaatc cctggccgtg gacaacgtct tcgtgtggct gatgctcttc
    16681 agcttcttcg gcattcccct agaactgcag aagcgggtgc tcacctacgg cgtgctcggc
    16741 gccctcgtgc tgcgcacgct catgatcctg ggcggcgcct ggctgatcgt ccagttccac
    16801 tggctgctct acgtcttcgg agccttcctg ctgctcaccg gcgtgaagat gctgtggctc
    16861 gccgacgccc gcccggatct ggccgccaac ccggtgctgc gctggatccg ccgtcacgtc
    16921 ccggtcaccg aggcgcttca cggcgagcgc ttcatcgtca ccctgcccgg caccaacggt
    16981 ccggtccgtg ccctcacgcc gctggccctg gccctggtga tggtcgaact gtcggacgtg
    17041 atcttcgccg tcgactccat ccccgccatc ttcgccgtca ccaccgatcc cttcatcgtg
    17101 ctcacctcca acctgttcgc gatcctgggc ctgcgggcga tgtacttcct catcgccggc
    17161 atggcggacc gcttcgccct gctcaaatac ggcctcgccc tggtgctcgt gttcatcggc
    17221 gccaagatgc tcctgctgga cgtggtgaag atccccatcg ccctgtcgct caccgtggtg
    17281 gcgctgctga tcggcggtgc ggtgatgctg tccctgcacc gcgaacggcg cagggcgctg
    17341 ccggagcctc gacgcgacga tgggggcggg cgataatgcg cggttatcag ccagccgccg
    17401 tccgagcggt ccgcgtccgc cccaggagag ccgccatgat ccccacccgc cagcacctcg
    17461 aactgctcgc accggccaaa aacgccgact tcggcatcga ggccatcaac catggcgccg
    17521 acgccgtgta catcggcggc cccgccttcg gcgcgcgggc ccaggccggc aacgaggtgg
    17581 ccgacatcga acgcctcgcc cgccacgccc accgctacgg cgcgcgggtg ctggtggccc
    17641 tcaacaccat cctccacgac gccgaactcg acggcgccga ggccctcatc cggcaggttt
    17701 acgaggccgg cgccgacgcg ctgatcgtgc aggacatggg cctgctggaa ctcgacctgc
    17761 cgcccatcca gctccacgcc agcacccaga ccgacatccg ccatccggaa aaagcccgct
    17821 tcctgcagga cgtgggcttc tcccagatcg tgctggcgcg ggagatgagc ctcaccgaga
    17881 tccgcaaggt cgccgccgtc accgactgcg ccctcgaatt cttcgtccac ggcgccctct
    17941 gcgtcgcctt ctcgggccag tgctacatca gccacgccca caccggccgc agcgccaacc
    18001 ggggcgaatg ctcccaggcc tgccgcctgc cctacgacct ggaagaccag gacggccacc
    18061 tcgtcaccgg caaccgccac ctcctgtcca tgaaggacaa cgaccagagc gccaacctgc
    18121 gcgccctggc cgacgccggc atcagcagct tcaagataga aggccgctac aaggacatcg
    18181 gctacgtcaa gaacgtcacc gcccactatc gcaacctcct cgaccagctc ctcgaagacg
    18241 cgcccgacta ccgccgcagt tcctccggcc gctgcacctt cctgttcacc ccccagcccg
    18301 aaaagacctt caaccgcggc gccaccgact acttcgtgaa cggccgccag ggcgacatcg
    18361 gcgccttcga ttcccccaaa cacgtgggcg agcccgtcgg cagcatcctg cgcatcgacc
    18421 cgcaacgccg cagcttcgag ctcagcagcg ccgtcgagct gcacaacggc gacggcctca
    18481 gcttctacga ccgcaagggc gaactcatcg gcctgcgcat caaccgcgcc gaaaagctcg
    18541 gtgacgactt ccgcgtgcac accgccgacg ccgtgcccgc cgacctccgc cctggcagcg
    18601 gcgtgttccg caaccatgac cacgccttcg agcggctcat ggacaagaaa accgccgaac
    18661 gccgcatcgc cgtggacctg cacctcaccg acgacaccga cggcctcgtc ctgcaggtca
    18721 ccgacgaaga cggcttcagc gcccaggcgc gcctgcccca tcccctcgaa gccgcccaga
    18781 acgccgagcg cgcccgcagc gccctccacg acggcctcgc caagctcggc gccacgatct
    18841 tcagcgcacg cagcatccgc ctcgacctcg ccacgccgtg gtttgtcccc gctgccgccc
    18901 tcaacggcct gcgccgcgac gccatcgaac acctcgaagc cgcccgcctc gccggcctgc
    18961 cacgcccgcc gcggcgcgcc gccgtcgaac cgccggcgcc ctatccccag accgaactca
    19021 gctacctggc caacgtcctc aacgaacgcg cccgcagctt ctaccggcgc cacggcgtca
    19081 ccctcatcga agacgcctac gaagccaacc aggtgctcga cgacgcctcc ctgatgatca
    19141 ccaagcactg cctgcgctac agcttcaacc tgtgccccaa ggaagtgaaa ggcatccgcc
    19201 ccgaccccat gaccctcgtc aatggcaagg aacgcctcac cctgcgcttc gactgcaagc
    19261 gctgcgagat gcacgtcatc ggccgcctgc gtcccgccgt gctggacgcc gtcccgcaac
    19321 ccgtgcgctt ccacgcccag gcgccggccg ccctgcgcgc cggccgctga gccgcccacc
    19381 accgcgatcc ctccatgaag ctcgccatca ccctcatcgt cctcgccctg ctgatcatcg
    19441 tcgggcccgc cgcctggcaa ttcacccaca ccaaccccgc cgccgtgccc acctccggcc
    19501 tgccctggca gatcgacacc ccggcgcccg gcgaatcccg cgtgttcagc ctcaccctcg
    19561 ggcgcagcac cctcgccgac gcccgcacgc gctttggccc cgacgcccag ctcgccatcg
    19621 tcgccacccc cagcgaagcc ggcaacgtgg agctctacta cgaatccatc accgccggct
    19681 tcatcgccgg ccgcctcatc gtcaccgccg acctgcccgc cgccaccgtc gaaggcctgc
    19741 gcgaacgggc cgccaagacc gaatacatgc agagcaccac gcgcaaggcc accctcgccg
    19801 aggccgacct ccctgtcgcc ctcgccgcgc cgatccgcgc catcgccttc atccccagcg
    19861 ccaacctgga cgaagccatc gtcgtcgaac gcttcggcgc ccccgccgaa cgcctgcgca
    19921 gcaacgacca catcgaacac ctgctctacc cggccaaggg cctcgacgtg gtgctcgaca
    19981 gtaagggcaa ggaactcctc cagtacgtcg ccccggcccg tttcgacgcc ctgcgcacgc
    20041 ccctgcgggc cgccgccgac caggccgcga ccgccccgcc cgccgcggcc cccgacaaac
    20101 cctgacggca caccgattgc ttgcccggca ggattccccg tccggagcat tgcccatgag
    20161 caccccgtcc accgtccgcc acttcgtcac ctacagcggc gtcagcctgc ccctcaagct
    20221 caccacgccc ctcgaaggtg ccgatctcgc ccaccgcatc accttctacc gtgcccacta
    20281 cgacgcggcc gagcgtctgg ccctcgtcga gaaagtcgtg tacggtgaga tcgaatccac
    20341 ccaccactac acctactacc ccagcggcgt gttgcaggaa gcccgggttt gtcagatagc
    20401 cgacgaagaa acgacactca tggcctacga cgaaaccggc gccctcgtct ccaccacccg
    20461 cctggaagac tgaatcccgc atacgtggcc gtccagtccg tccaacgccg ccgctgtctc
    20521 acctgcaacc gctggggcgg acagcgccgg ccgggcgacg aacccgacac cgtcgaattt
    20581 gacgaagccc acgaccgcgg tccctgccag gacggcccct ggcacggcac cctgcgcggc
    20641 ccgcgcaatg cctgcggcca gtggctgcgc tggaccctga tcgtgcctgc tcccgacccc
    20701 gccgaagcgc ctgcccaagc gtccccgccc catgaacagc cctgacgatt ccgatctcct
    20761 cgtcgcctgc ctgtgcgccg cctggtgcgg cacctgccgc gactacgccg ccggcttcca
    20821 cgccctcgcc gaacgcttcc ccggcgcccg cttcctgtgg ctcgacgtgg aagacgaggc
    20881 cgatctcctc gacgactacg acgtggagaa cttccccacc ctcctcatcc agcgcggcga
    20941 cgcggtgctt ttcttcggca ccatgctgcc ccatcatgag ctcctcgaac ggaccgtgcg
    21001 cgcctttgcc agcatgacgc ccgacgaaag ccgccactac gcccaggccg acgccacccg
    21061 gcgcacctgg cagcacgacc gcaacctgcg tcgcgccctc gccgaacggg acggcgacgc
    21121 ctaaaacaag cacaacggga gacaccccat ggacacctgc gttttctgcc gcatcgtgcg
    21181 cggcgaactg cccgcctccg tcattcacga agacgaacac accctcgcct tcatgaacat
    21241 catggccggc aaccccggtc acgccctggt catcgtcaaa ccccacgtcg aaaacatcta
    21301 caccctcgac gacgccctcg ccgccgccgt cttccgcacc accacccgcg tcgccaaggc
    21361 cgtgcgcgct gccgccggct gcgaaggcgt cagcctcttc caggccaacg aagtggccgg
    21421 cgggcagacc gtcttccact tccacatcca cgtcctgccg cgcctccccg gcgacgccct
    21481 gcccggcgtg tggcccgccg ttgccccaag ccgcgacgcg ctggacgcca tggccgcccg
    21541 cctgcgcgcc gccctgtaag gcacgcgccg ccgcccgccg caccggcatt caattccgcc
    21601 ggcccgccgg ccgataaact tcccgtccac cgcgccgccg cctgccgtgc atcccgcccg
    21661 gcccgcggcg gatgcctcca tccggtcgcc gccatgcaca tccccctgct cagcgccgaa
    21721 gcccccatca ccctgatcct catggtgatc gccggcctca tcatgctccg gcccctcgcc
    21781 catctgcgcc gctccctgat gaacaccggc gtgttctacg gcgcctgctt tgctggcgac
    21841 ctcctcgccg gcctcctcga aggcagcagc cagcccgccg ccgcccgctg gctgcgcgaa
    21901 ctctgcgtct ttggcgaagg gctcgccgtc gtccgcttcg tcggcctcac cctcttccag
    21961 gtgatcctgc cgcgcctgca cgtcaacatc tccggcatcc tcgccgacat cctcgtcatc
    22021 gtcggctaca tcctgtgggg cttcatccgc ctcagcatga gcggcgtcga attcaccgcc
    22081 ctgttcacca cctccgccgt cctcaccgcc gtcgtcgcca tctccatgca ggagaccctc
    22141 ggcaacctcc tcggcggcct cgccctccag atggacaact ccctcgagat cggcgactgg
    22201 atccagatgg gcgacctcgc cggccgcgtc accgacatcc agtggcgcta caccgccatc
    22261 gtcacccgcg ccggcgaacg catggtggtg cccaacagcc acctcatgaa gaaccgctac
    22321 gccatcgtct gcgacaagcg ccagtccaat ccccaccagc gccgccacat ccccttcaac
    22381 gtggatctct ccgtaccccc cgcgcgggtc atcggcgccg tgctgcagga catcctcacc
    22441 gcccgcatcc ccaacgtcag cgccaccccc gcccccgcct gcctgctcat ggacttcggc
    22501 cccggctttg cgcgctacca gctgcgctac tggctcgacg accccggccc cgacgaaatc
    22561 accgactccg acgtgcgcag ccacatcctc gccgccctcc agcgcgaaga catccgcctc
    22621 gccgtcgccg accagaccgt ccacctcgtc caggaaggca aagcccaccg cgccgaagtc
    22681 caggcccgcg aactcaaccg tcgcctccag gccatcaccc acatcgacct cttctgcaaa
    22741 ctctccgaag ccgaacagct cgaactcgcc cgccgcctcg tcaacgcccc cttcgcccgc
    22801 ggcgacatcg tcacccgcca gggcgccgtc gcccactggc tctacatcat cgtcagcggc
    22861 gaagccgaag cctggtggca accgcccgac ggcagccccc agcgcctcct cgaaaaacgc
    22921 ggccccggca ccgtcttcgg cgaactcggc ctgatgaccg gcgccccccg ccgcgccacc
    22981 gtcatcgcca ccaccgacgt ggaagcctac cgcctcgaca agaccggctt cgaacacatc
    23041 atccgcgccc gccccgagat cgccgaaggc atctccgcca tcctcgaacg ccgcctcagc
    23101 cacttccagg ccctcgaacg gggcgacggc gccccgcgcc ccgacggcaa caaacccgcc
    23161 aacggcgaca ccggcgtcat cggccgcaag atccgcgaat tcttcgggct gtagacgcac
    23221 caaggccccg ccgcccttgc ggagacgggg ccccagcacc tcaacacatt gcaggcatcg
    23281 gcttccgccg atcccgcgcc gcattcaggc attcactgcg cgggtgacgg cgtaaacgcc
    23341 ttcttcgctt tctcctccag ttccccagat accgtgcggt tcgccgccag ttgttccgcc
    23401 gccgtccccg tggcaaagaa ctgggacaac tggaacttcg ccgtcgcacc ggacgatccc
    23461 gtcgtggcgg ccggcgccga cacccccgcc gacgtattca ggctcgcaaa caccgacggc
    23521 tgccgcgtct gatccaccgg aatcaccgac gcctccttgc gcttgtagcc aaaggtgaag
    23581 gattccggcg catagttgct cgcctggaag gccagcttca gccccagcac cgtggacgta
    23641 gcaaagaaca gcaccggcgg actgcccggc gacggccccg ccgccacggt cgctgcaggg
    23701 ggagccgccg tccccgccgt catcaactcc gccgcccgcc ccgtcgcata catctggcga
    23761 atctcccgga ggatcccctg cccttccgtc tcgatcaccc cgaccaccgg aaacaccttg
    23821 ccgttgtcaa agcgcggccc cgcgtagccc tccacccggt catacgcaat gctcaccccc
    23881 gccggcaccg tctccgcatc aaacaccgac agcgaagtct tcgtcacgaa gatcgcattc
    23941 tcccgcggcg acgcacacgc ccccagcgtc aacgccagta ccgccagcgc cggtccgctc
    24001 acactccgtc gattcgtttt catcgtctca ctccttgact gttggggctt ctacaggcga
    24061 caccggctcg gccgacaccg ccatctccgg cgcaaaacac gcgttctctc gcaccgccac
    24121 gtgggtggca tcgctatagc ccagcaccag cgcgtgactc tcgtggctgc tgctcaccga
    24181 cacccccagc gaacgcaccc gcaaggattc gcccccccac ggcgccggat tgcccggcgg
    24241 aatctccaga tgaacaaatc ccaccacatg ccgcgtcccg tccggcgcca cgtagaggta
    24301 ggaacacgcc gccggcagca ccagcagcgc acagcccagc ccgcgcaatg cagccggaat
    24361 ccatcgccct accgcccgcc atgcggccat gct
//
LOCUS       DAOQPA010000043        24183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_989_length_24183_cov_16.194463, whole genome shotgun sequence.
ACCESSION   DAOQPA010000043 DAOQPA010000000
VERSION     DAOQPA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_24183_cov_16.194463"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PKX01_07970"
     CDS             complement(<1..1378)
                     /locus_tag="PKX01_07970"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76099.1"
                     /translation="MNTLWFSLRRRLLGLLLGGVSVAWGVTMVFSYVDAHHEVDELFD
                     AQLAQAGQTLLVLADQGGGRAVSRMEEAVHKYQRRLRFQIWDADGQLAMRSETAGTAP
                     LTPIDGFSETEDGAGHWRHFSQWNSARRLQVQVSEDHRIRDDLIAHIAWRIVLPALFG
                     LPLLGLGVWLATRRGLASLDVVAGQIASRAPEQLQAVSPASAPEEIRPMLEALNGLFR
                     RVDSALEAERRFTADAAHELRTPLAALQTQLQVALRARDEAERARSLAQIQSGLARAA
                     HLVEQMLQLARLDPESGLPDPRAVDLAALAEDVCAELGSAILARGLEFELAAEPGCVV
                     FGQAEWLRVLLRNLVDNALRYTPEGGRLRVGVARAAEGPGGGAIVLGVEDDGPGIPPG
                     QRESVLARFVRLEAGSAPGSGLGLAIVARIVDLHGARLALGSGAGGRGLRVEGRWPEV
                     VKQWEAVQQ"
     gene            complement(1375..2031)
                     /locus_tag="PKX01_07975"
     CDS             complement(1375..2031)
                     /locus_tag="PKX01_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76100.1"
                     /translation="MRILLAEDDPQLGDGLTVGLRQAGFTVDWVRDGQAADVALQAEA
                     FDLLVLDLGLPRLGGLDVLARVRGRGQAVPVLILTARDAVGDKVAGLDGGADDYLVKP
                     VDLDELAARIRALIRRSAGRSAPLLVWGELVLDPAARRLTRAGEPVELSGREFALLQM
                     LLEGAGRVLTRSQLEQSLYGWGDEPDSNALEVHIHHLRKKLGSELIRTLRGVGYTIPR
                     "
     gene            complement(2064..3359)
                     /locus_tag="PKX01_07980"
     CDS             complement(2064..3359)
                     /locus_tag="PKX01_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ76101.1"
                     /translation="MSEPLPRLAIACQGGGAHTAFTAGALSYLLLAFEHFRRQPGGRV
                     PFQLTGLSGTSGGAITAAMAWSDPGAGGWAEGAQRVLRFWNRNKASLQFQDKDPWWWG
                     EFFTNASARWLANFEDFGPTVSWPPNPLTSTLTQDRMRTDIRAALDLPPEADAIRGRA
                     GVDLYVGAVDVINRDAVSPGSAFRTFPATAGDALHVDELLASACIPELFFAQKLKIRS
                     GRHRESYFWDGLYSQNPPLTNFFCNREAEHKPDLVWIVQINPSSYSGRAPMTPSQIDD
                     RRNELAGNLSLGQELRSIATVNQIAAELDALRAQLPADDAALRAPLFSRYKTVTVAFL
                     PLANEDDSGRPLAAYTLDHASKMNRDPAFIDALIAEGIATAHTLAHAGQLVRPACMTG
                     ERHARHFPNPSWPAPAELVPLLNDPVLAAQWRALAAGIA"
     gene            3559..3909
                     /locus_tag="PKX01_07985"
     CDS             3559..3909
                     /locus_tag="PKX01_07985"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ76102.1"
                     /translation="MSADALSATFAALADPTRRAILARLATGEATVSELAEPFAMSLP
                     AVTKHLKVLQRAGLVSQGRRAQWRPCRLEARPLQDAADWVGQYRRFWDERLDRLEDYL
                     RTLNALEPPHDDTP"
     gene            3893..4393
                     /locus_tag="PKX01_07990"
     CDS             3893..4393
                     /locus_tag="PKX01_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ76103.1"
                     /translation="MTTPPDASAAPASERELRLTRELDAPPALVFRLWTEGEHLARWC
                     APKGFTIPEGGGDVRPGGAWWSVMVAPDGTVHRLGGNYLQVDPPRRLTFTHAWRDAAG
                     QPGHQTIVTVTLDDLGNGRTRLTLHQARFDSVPARDGHSGGWRECLDRLDALARQLVQ
                     QQAQQQ"
     gene            4428..4946
                     /locus_tag="PKX01_07995"
     CDS             4428..4946
                     /locus_tag="PKX01_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ76104.1"
                     /translation="MTAPLTGESAEFHIERLIDAPREQVWQAHTQAEHLARWWGPKGL
                     GLRVLSLDLRPGGRFHYAMVAPDSSETYGRFVYREILAPARLVWINSFADAAGEAVRH
                     PMAPDWPLHMRVQVDLSEADGRTRLVLRSRTLDASAAECATFEAGFDSMRGGFGGTYD
                     QLDAHLATFNRS"
     gene            4970..5389
                     /locus_tag="PKX01_08000"
     CDS             4970..5389
                     /locus_tag="PKX01_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ76105.1"
                     /translation="MYANAYLFFEGRCEEALQFYQDALGARVEMLLRNRDNPQPPPPG
                     TMPPGTEDKIMHAALRIGDTLVMASDGYCHGGASFQGFALSVSPDSPAEAERVFAALG
                     VGGTVRMPLGPTFWSPAFGMVHDRFGVLWMVNVMPAG"
     gene            complement(5395..7038)
                     /locus_tag="PKX01_08005"
     CDS             complement(5395..7038)
                     /locus_tag="PKX01_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ76106.1"
                     /translation="MTTPAPPDSDRFSDLLAGVSLAGLLLPEAVAYSSIANLPPQAGV
                     MALFAGLLAYGALGTSRFAVVSATSSSAAVLAAVTASLAGGDAVLRGALAGGMILLAG
                     VFFVLAGLARMGNASDFISKPVLRGFAFGLALTIALKQLPKMVDVHPAQGDLLHFVPA
                     LFGHVADWNLAGLGLGGASLALLFVLERLRPVPGALVVTALGIVLARTVDLAAWGVDT
                     VGPIRLHLAAPDLPDLSRAAWLRLAEVAVAMVLVLYAESYSAIRNFALRHGDAVDVNR
                     DLLALGVANLASGLVHGLPVGAGYSATSANEAAGARSRLSTWVCAAVALLIVLTLLPL
                     IADTPEPVLAAIVVFAVSHSLDPGVFRPCFRLHRDRIVVVAAVVAVLLLGVLDGLLAA
                     IGVSLMMTLKRLSDPALSELGRLAGGHDFVALKLHPEAEALPGILILRPETPLFFANA
                     GHVVTGIRRTLEGHAGLHALIVSLEESPDLDSTSVEALEELAAATRRAGVGLYFTRLK
                     PAALTVLRRAALPGLPPDALSDLSVDDAVTLALSAPLVR"
     gene            complement(7085..9811)
                     /locus_tag="PKX01_08010"
     CDS             complement(7085..9811)
                     /locus_tag="PKX01_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HMZ76107.1"
                     /translation="MPGFVHSRAVVWGLVLGLLAAFAWGAPVAAAGDEAQADVPARVA
                     VLAFRPKDETRARWMPLIDYLNAAGLGRRFELLVMTDTEIDAAVRARQVDVVLTQPAH
                     YVALSTREGLLSPLATLVENEAGHPLARFGGVIVVRADDARLRSVDDLRGKRLVTAAR
                     SSLGGYLMQAGMLQRRGIRLPQDATVTETGYPHDTALAAVLAERADAAFVRSGVIEAK
                     VRDGSLDGAKLRVLDPVADETYPFQTSTPLYPQWPVAAMPWTDEDLARRMAATMLGLP
                     HGGETANRIGLHGFTIPGDYRPVEDLMRRLRVAPYDQVPEFTLVDVWDHYSVLLVSLL
                     AAGMTVLAAAVLLLVRANGRLRAERLRTDEALQRLTEAEAEQRLILDALGEGVYGIDA
                     DGACSFANPAALKLLGLTEGDLLGRDTHALIHHHYPDGRPYPHAECAAHQTARDGMPR
                     RHDDWFWRSDGSGFPVRMTVTPRLENGEVCGTVVVFSDISESVRVARELDAHRDRLEE
                     QVRQRTAQLEQARAAAEASNRAKSEFLANMSHEIRTPLNAITGMAYLLRRSLLTPAQA
                     ERLDKIDAAGRHLLDIIDAILDLSKIEAGKFALQDGGVDLVRLTGNVLTMLTDRAQAK
                     GLSLVCEPVPALPPLRGDAARLQQALLNYLANAVKFTERGRIVLRVRVVADEGDHLRI
                     CFEVEDTGIGIAPDVLPALFLPFSQADSSITRQYGGTGLGLTITRKLAELMGGTAGVE
                     STPGRGSTFWFSARLAKAATAVGAACGPSVSGAEAQLRQRHAGRRVLLVEDEPVNREI
                     ASELLQDAGLAVSTAEDGIAALERLAAERWDLILMDMQMPRLDGLETTRRLRARPELV
                     GLPVIALTANAFADDRARCLNAGMDDFVAKPVDPEALYATVLKWLDAPRSGA"
     gene            9990..11204
                     /locus_tag="PKX01_08015"
     CDS             9990..11204
                     /locus_tag="PKX01_08015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ76108.1"
                     /translation="MDRAGLVCQELVSQQTSLRVAVVTETYPPEINGVAMTTGRMVDG
                     LVAAGHRVDLIRPRQAHEGGVVLDADGTDGVEEMLSRGIPIPRYANLQLGLPARKVLL
                     RRWSVQRPDVVQVVTEGPLGWSALAAARKLHLPVISEFHTNFHRYSGHYGIGWLKRPI
                     AAYLRKFHNRADLTLVPTRALRDELAGHGIPRVDVVSRGVDTLLFDPARRSAELRRSW
                     GVGPDDLVVLYVGRIAPEKNLALLEQAYVALKARVPAARLVMVGSGPAQAGMRGRLPD
                     VIFAGPQVGEQLAAHFASGDLFLFPSLTETYGNVVAEALASGLPVVAYRDAAALELIT
                     PGVHGHLAEPGDERGFVHGAVQLAESPELRARMRLAARERVASLAWRAIVDSLVSLQR
                     QVILSSPLSHRR"
     gene            11209..11733
                     /locus_tag="PKX01_08020"
     CDS             11209..11733
                     /locus_tag="PKX01_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ76109.1"
                     /translation="MFALPARLQLIDDRLAVRSNRLSRYRGLRDFLRVVSRLGDGVAW
                     YTLAAALYGLFGAEALPALTQMLAAGVSGVIVYKLLKNHTLRPRPFEVVSDVVCAGSP
                     LDRFSFPSGHTLHAVSFSLILAHHFPPLAGAVFGFALLVAISRPVLGLHYPSDVLAGG
                     AIGASLASATLTLL"
     gene            11959..13299
                     /gene="guaD"
                     /locus_tag="PKX01_08025"
     CDS             11959..13299
                     /gene="guaD"
                     /locus_tag="PKX01_08025"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HMZ76110.1"
                     /translation="MPSTDTTAHPASILVRGQILHFLADPGPEDDPAAWEYFDDGALW
                     IVDGRIHAAGPWEALHAALPPARRAAATYYDYRGRLVLPGLVDTHLHYAQAGALGSFG
                     RDLLDWLGGAIFPAEASFADPGVAERTAAFVADRLLAHGTTTASVFATVHAHSVDAFF
                     AAAEARQMRVLCGKVLMDRHCPDALRDTAASGAADSLALIERWHGRGRLRYSLSPRFA
                     PTSTPEQLQRTGELFAAWPDLHLQSHLAESHGERAWIARLFPGCRDDAEVYARYGLLG
                     PRAIYAHALHLDDSQRRALAATGTAVAFCPSANLFLGSGFFDYAASTAAGLRVGLASD
                     VGAGTSFSLIRSLGRAFDVSQATGAPLSALRGWFLATLGGAQALSLDDCIGSFQPGRE
                     ADFVVLDLAATPELAWRMGQARSLTDQLFALMILGDERCVVATHLLGRRRWARP"
     gene            complement(13331..15433)
                     /locus_tag="PKX01_08030"
     CDS             complement(13331..15433)
                     /locus_tag="PKX01_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMZ76111.1"
                     /translation="MRANLPVTPDEYHLPPGAAIISRTDIQGTIIDCNEEFVRASGFE
                     RMELIGQPHNMVRHPDMPEEAFRDMWATLKRGRPWSGVVKNRRKNGGFYWVRANVTPL
                     MDGAGYMSVRVAPSRVDVQAAEALYARMRKDRSVRLHEGQVARTGPLLRVTRRLMRPW
                     RRSVGARVMSGNAVLLLLLALTAWWASGVIHSSGVDGDRFGRIVQGKDLLADILPPPA
                     YIIESYLVAREMRDQQGAELDAARSRLLKLKQEYDERHAVWQAADLPPELRKALLEEA
                     DAPVAPFYALATGDYYAALRDGNAARAQEALNTLQGLYGAQRKAIDAVVNGTNRWNDA
                     LVGESRDFVTEARTRLGGATALALAVAVVFSALAIRSVVKPLAAVGRAADEIARGNLL
                     CELPQAGEDEIGILVARLALMRNNLHEIAAALRQEAANLKGSLVQMRESAQVSATRAE
                     EQSSYAGALAAAIEELSVSIDHINDNTIRAHGLSADARDGAVEGGQIMVSVSDEISRM
                     SASTQEVVGTMQALEAFAADITKVVDVVSGIADQTNLLALNAAIEAARAGEAGRGFAV
                     VADEVRKLAERTGNSTREITTMVEKVIDGTSKAVADMDANVGGVASVAERARSAGESV
                     TTISGSSVAVLAAMEEIKLGLGEQSVAAREIASRVEQIASVSEANAATASQMRETSRR
                     MAGLAEELQGLTGRFRIA"
     gene            complement(15654..16130)
                     /locus_tag="PKX01_08035"
     CDS             complement(15654..16130)
                     /locus_tag="PKX01_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ76112.1"
                     /translation="MDVVADQDSPRWDHSLDSRVVPLFLALQGARARSLETMRPVLAR
                     YDLSLAEFDVLATLRNAPPPHELTPSQLQAEVVITSGGLTKVMLHLEGRGLVLRRLAA
                     DDARVKPAALTPAGAAQIEAAMAEVVAATGAWLRAALSDAEMARLQTVLARLAGAG"
     gene            16239..17573
                     /locus_tag="PKX01_08040"
     CDS             16239..17573
                     /locus_tag="PKX01_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HMZ76113.1"
                     /translation="MKAIQRTLAGFLLLLTAGWALSEPGDLVAIASFFDFRSLFMQWS
                     GVIAIGVMSLAMILSLRPAVLEPYLGGLDKLYRLHKWLGITALGITLAHWLMAKSPKW
                     LIALGWLERRGRRPRPSFPEGSLQGLFAGLHGLAEDVGEWAFYGALVLMLIALIKAFP
                     YRRFVQTHRLLPLAYLALVFHAVILLKFDAWRGPIGPLLVALMALGSVAAVSSLLGWR
                     PGRRPVTGTVTAVAADAIDPVLTVDLRLDPGWPGHTAGQFAFVTFDSREGAHPFTLAS
                     AWRGDGYIQFRIKALGDYTRTLAARLHPGARVSLEGPYGRFTFGGNAARQIWIAGGIG
                     ITPFLARLQALAATPDGRRTDLFHCTARYSADAVRQLRHAADAAGVRLHMLWDERDGR
                     LDAARLAETVPDWRDADIWFCGPAPFGDALRTGLVRLGLPARRFHQELFEMR"
     gene            17900..19186
                     /gene="nhaD"
                     /locus_tag="PKX01_08045"
     CDS             17900..19186
                     /gene="nhaD"
                     /locus_tag="PKX01_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HMZ76114.1"
                     /translation="MLTVLILIFAVTYLAIALEHPLNLHKSASALAGAGLMWTIYALL
                     GPDAATVAHQLDDTVTATAQIVFFLMGAMAIVEVIDAHHGFDVLTRRIRTTQQTTLLW
                     LVGSATFVLSAVLDNLTTTIVMISLLRTLLARHEDRLVFAGLVVIAANAGGAWSPIGD
                     VTTTMLWIGGQLSAAAIIRSALLPSLVCTLVPLLVLSLALRGRPVVAPDAPDPATAGP
                     GATEFERRFMFALGIGVLVAVPAFKTLTHLPPFLGILFGLGIVWLAGDLIHRDKSAEY
                     RERLTLAHALGRIDLGSVVFFVGILLAVATLEHTHLLSALAAWLDLRVGREDLIVLLI
                     GMASAVVDNVPMVAGAMGMYPLADYPADHFLWRFLAYCAGTGGSVLIIGSAAGVAAMG
                     MAHIPFGWYLRRISGLALLGYLAGAGVFVLQQGVAA"
     gene            complement(19229..20557)
                     /locus_tag="PKX01_08050"
     CDS             complement(19229..20557)
                     /locus_tag="PKX01_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ76115.1"
                     /translation="MRRSMSVGVLGLVLGLGNALAQQPGSLRPQVEAQAQRLEAQVIA
                     WRRDIHQHPELGNREFRTGRLVADHLRALGLEVRAPVAHTGVVGVLRGGLPGPVVALR
                     ADMDALPVTEQSGLPFASTQKARWQGQEVGVAHVCGHDGHTAILMGVAQVLASLRERV
                     PGTVVFLFQPAEEGPPPDEEGGAALMIREGALKDPAPSAIFGLHLNSRTPLGKAGVRG
                     GPFMASGDTFRIVVQGSQTHAALPWLGVDPVVLAAQVVMGLQTIESRQVNVLLEPSVI
                     TVGAINGGNRPNIIPDTVELSGMIRALDETMRGDIRKRLIRTAEKIAESGGGSAAVTI
                     TPNYDVTVNDERLARLTAESLRATLGRDQVFVNPKVTVSEDFSAYQKLVPGVFFFLGV
                     VPPDARPETVFPNHSPRFWIDERVLAIGVRTLTNVALDYLTAAAAPAAGD"
     gene            complement(20724..21197)
                     /locus_tag="PKX01_08055"
     CDS             complement(20724..21197)
                     /locus_tag="PKX01_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ76116.1"
                     /translation="MTSADTTLGQDGAGGAAGLDFSWDIERVDWPALVALFERAKLGR
                     RREPERLETAFRNSALCLFVYAGPVLIGVGRALSDGVSRAALYDVAVLPEYQGRGIGQ
                     AIVRRLLAAANVELTMLYAAPGKEAFYAQFGFRRMRTAMAIMPDEADARARGLIE"
     gene            complement(21209..21682)
                     /locus_tag="PKX01_08060"
     CDS             complement(21209..21682)
                     /locus_tag="PKX01_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ76117.1"
                     /translation="MILSGHGLDEVDRTVLRLLQGDGRMSNARLAETLSLSETPCWRR
                     LRRLEAEGFIEGYQARLSRRKLGYGVLAFVQVTLGDHAGEAPLQFERQVAGLPQILSC
                     HNVTGDCDYLLQVVAEDLDAYGLFVRDRLRTLPGVTAIRSSLSLREVKASSALPV"
     gene            21705..22448
                     /locus_tag="PKX01_08065"
     CDS             21705..22448
                     /locus_tag="PKX01_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HMZ76118.1"
                     /translation="MKLSEQWLFTKDLAILFPFIGPIMKLPHRPTKPTAVIDTAALPL
                     FLAALAAAYLLPGPDMALIVSTTARHGPRSGLMAAAGLALSRSLHVLLSGFGLAALFA
                     AHPLLFHGVKLLGAAYLLGLAWTLLTAPDTTPGATAAAACASPPGAADALRRGFLTNL
                     LNPKALGFCAILLPQFVAPHQPLPGQFAVLGLILVGLGAAFDAAYICAAQGVARRFAA
                     APRLRGLSRLTCAGVFGLAALRLAFSGTD"
     gene            complement(22490..23257)
                     /locus_tag="PKX01_08070"
     CDS             complement(22490..23257)
                     /locus_tag="PKX01_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ76119.1"
                     /translation="MDFVVDAHMGLALVVGAGLGFFGGLFGIGGGILAIPLFVLGFGM
                     EQAQAQGTALVLMVPNLLVGWWRYQQRQPMPWRAQAAIAGSATLVTWGMAHFATRLDP
                     LVLRGVFCVFLLALAIRLWRAAPAAAGAEKPLALRWLPVVGAAGGCSMGLLGIGGGLL
                     ATPLLAGWLGQRQAVAQGLSLALVAPSSAVALAAYASAGHVDWSRGLPMAVGGLATVS
                     AGVAWAHRLPERRMRTAFAAMLGVTALWLGVGRWLMG"
     gene            23357..>24183
                     /locus_tag="PKX01_08075"
     CDS             23357..>24183
                     /locus_tag="PKX01_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76120.1"
                     /translation="MNDLPDLAFFALLARQPTLAAAAQALGVTPSSVSRRLAALEHRL
                     GVRLLHRTTRRISLSAEGSRYLEGGEQILHDLDNLERSLLGSREMPRGLLRINATFGF
                     GRRHLAPALSDFARRYPDVEVILDLTDRPLDLTEHAVDVGIRFGPPPDARIQARRIAP
                     NRRLVCAAPAYLDAHGHPESPRDLPRHRCIVIREATAAYNHWQLTDGHEQIMVKVGGP
                     LSTNFGEIAVEWALAGHGIVLRSEWDVAADLRDGRLQRLLSPWAGTSADIHAVYPPR"
BASE COUNT         3397 a         9512 c         7885 g         3389 t
ORIGIN      
        1 cctgctgcac cgcctcccac tgcttcacga cctccggcca gcgcccctcg acccgcagcc
       61 cgcgcccgcc ggcgccgctg cccagagcca ggcgggcgcc gtgcagatcg acaatgcgcg
      121 ccacgatggc gaggcccagg ccgctgccgg gggcgctgcc ggcctccagg cggacgaagc
      181 gggccagcac ggactcgcgc tgcccgggcg ggatgccggg gccgtcgtcc tccacgccga
      241 ggacgatggc gccgccgccc ggcccctcgg ctgcccgcgc cacgcccacc cgcagacggc
      301 cgccttccgg ggtgtagcgc agggcgttgt ccaccaggtt gcgcagcagc acgcgcagcc
      361 attccgcctg cccgaagacg acgcagccgg gctcggcggc cagttcgaat tccagcccgc
      421 gggccaggat ggcgctgccc agctcggcgc acacgtcctc ggccagggcg gccagatcca
      481 ccgcccgcgg atcgggcagg ccggattccg ggtcgaggcg ggccagctgc agcatctgct
      541 cgaccaggtg ggcggcgcgg gcgaggccgc tctggatctg cgccagggag cgcgcccgct
      601 cggcctcgtc gcgggcgcgc agggccacct ggagctgggt ctgcagggcg gcaagggggg
      661 tgcgcagctc gtgggcggcg tcggcggtga agcgccgctc ggcctccagg gcgctgtcca
      721 cccggcggaa gaggccgttg agggcctcca gcatggggcg gatctcctcc ggcgcggagg
      781 ccggcgagac ggcctggagc tgctccgggg cgcggctggc aatctgcccg gcgaccacgt
      841 cgagggaggc cagcccgcgc cgcgtggcca gccacacgcc gagacccagc aggggcaggc
      901 cgaagagggc gggcaggacg atgcgccagg cgatgtgggc gatcaggtcg tcgcggatgc
      961 ggtggtcctc gctgacctgc acctgcaggc ggcgggcgct gttccactgg ctgaagtggc
     1021 gccagtggcc ggcgccgtcc tcggtctcgg agaagccgtc gatgggcgtg aggggcgcgg
     1081 tgccggcggt ctcggagcgc atcgcgagtt gcccgtcggc atcccagatc tggaagcgca
     1141 gacgccgctg gtacttgtgc acggcctcct ccatgcgact cacggcgcgg ccgccgccct
     1201 gatcggcgag gaccagcagg gtctggccgg cctgggcgag ctgggcgtcg aagagttcgt
     1261 ctacttcgtg gtgggcgtcc acgtagctga acaccatggt caccccccag gccaccgaca
     1321 cgccgcccag cagcaggccg agcaggcgcc ggcgcaggga gaaccacaag gtgttcatcg
     1381 ggggatggtg tagccgacgc cgcgcagggt gcggatcagc tcgctgccga gcttcttgcg
     1441 caggtggtgg atgtgcactt cgagggcgtt gctgtccggc tcgtcgcccc agccgtagag
     1501 ggactgctcc agctggctgc gggtgagcac ccggccggcg ccctcgagga gcatctgcag
     1561 cagggcgaac tcgcggccgg acagttccac cggctcgccg gcgcgggtca ggcgccgcgc
     1621 cgccgggtcg aggaccagtt cgccccacac cagcagcggg gcgctgcgcc cggcgctgcg
     1681 gcggatcagg gcgcggatgc gggcggccag ctcgtccagg tccaccggct tgaccaggta
     1741 gtcgtcggcg ccgccgtcca gcccggccac cttgtcgccc accgcgtcgc gggcggtgag
     1801 gatcagcacc ggcacggcct ggccgcggcc gcgcacgcgg gccagcacgt ccagcccgcc
     1861 caggcgcggc aggcccaggt ccagcaccag caggtcgaag gcttcggcct gcagggcgac
     1921 atccgcagcc tggccgtcgc ggacccagtc cacggtgaag ccggcctgac gcaggccgac
     1981 ggtgaggccg tcgccgagct ggggatcgtc ttccgcgagg aggatgcgca tgggcggagt
     2041 gtagcgggcc gcggccccga cgttcaggcg atcccggcgg cgagggcacg ccactgggcg
     2101 gcgaggaccg gatcgttcag cagcgggacg agttcggccg gcgccggcca ggacgggttc
     2161 gggaagtggc gggcgtggcg ctcgccggtc atgcacgccg ggcgcaccag ctgtccggcg
     2221 tgggcgaggg tgtgggcggt ggcgatgcct tcggcgatga gggcgtcgat gaaggccggg
     2281 tcgcggttca tcttggaggc gtggtccagc gtataggccg ccagcgggcg gccgctgtcg
     2341 tcctcgttgg cgaggggcag gaaggccacg gtgacggtct tgtagcggct gaacagcggc
     2401 gccctgaggg ccgcgtcgtc ggccggcagc tgcgcgcgca gggcgtccag ctcggcggcg
     2461 atctggttca ccgtggcgat actgcgcagc tcctggccga gggacaggtt gccggccagc
     2521 tcgttgcggc ggtcgtcgat ctgggacggg gtcatgggcg cccggccaga gtaggaggac
     2581 gggttgatct ggacgatcca gaccaggtcc ggcttgtgct cggcctcgcg gttgcagaag
     2641 aagttggtga ggggcgggtt ctgggaatac aggccgtccc agaaatagct ttcccgatgg
     2701 cgtccgctgc ggatcttcag tttctgggcg aagaacagtt cggggatgca ggcgctggcg
     2761 agcagctcat ccacatgcag cgcatcgccc gccgtggcgg gaaaggtccg gaaggccgag
     2821 cccggcgaca cggcgtcccg gttgatgaca tcgacggcgc ccacgtacag atccaccccg
     2881 gcacgcccgc ggatggcgtc ggcctccggc ggcaggtcca gcgcggcgcg gatgtcggtg
     2941 cgcatgcgat cctgggtgag ggtcgaggtc agcggattgg gcggccagga gacggtgggg
     3001 ccgaaatcct cgaaattcgc cagccagcgg gcggacgcgt tggtgaagaa ttcgccccac
     3061 caccagggat ccttgtcctg gaactgcagg gacgccttgt tgcggttcca gaagcgcagc
     3121 acgcgctgcg ccccttctgc ccagccgccc gcgcccggat cgctccaggc catggccgcc
     3181 gtgatggcgc cgccggaggt gccggacaag ccggtcagct ggaaggggac gcgcccgccg
     3241 ggctggcgcc ggaaatgctc gaaggccagc aacaggtaag acagcgcccc cgcggtaaat
     3301 gccgtatgag ccccgccgcc ctggcaggcg atggcgaggc ggggaagggg ctcggacatg
     3361 atggactcct ggaaacggtc tgcgccacgg cggggacacc ccccatcgcg cgcaacagcg
     3421 gcagatggtg ccgcattgta atgcgtgcgt ccttcagcct cctgtcaggc gccgcggcag
     3481 acgccaccga ttcttcttgc ggattccgcc gccggcatct aatttaccaa gtggttaatt
     3541 aaagatccgg tcctccccat gtccgccgac gccctcagcg ccacctttgc cgccctcgcc
     3601 gacccgaccc gccgggccat cctggcgcgg ctggcgacgg gcgaggcgac cgtctcggag
     3661 ctggccgagc ccttcgccat gagcctgccg gccgtcacca agcacctcaa ggtgctgcag
     3721 cgcgccggcc tggtcagcca gggccgccgc gcccagtggc ggccctgccg gctggaggcg
     3781 cgtcccctgc aggacgccgc ggactgggtt ggccagtacc ggcgcttctg ggacgaacgc
     3841 ctggaccgcc tcgaagacta cctgcgcacc ctcaatgccc tggagccgcc ccatgacgac
     3901 accccctgat gcttccgctg cccccgcctc cgagcgggaa ctccgcctca cccgcgagct
     3961 cgacgcgccg ccggccctcg tcttccgcct gtggaccgag ggtgagcacc tcgcccgctg
     4021 gtgcgcgccg aagggcttca cgatcccgga aggcggcggc gacgtgcgcc cgggcggtgc
     4081 ctggtggtcg gtgatggtgg cgccggacgg caccgtgcat cgcctcggcg gcaactatct
     4141 gcaggtcgac ccgccccgcc gcctgacctt cacccacgcc tggcgcgatg cggccgggca
     4201 gccggggcac cagacgatcg tcacggtgac gctggacgac ctgggcaatg ggcgcacccg
     4261 cctgacactg catcaggccc ggttcgattc ggtgcccgcc cgcgatggcc acagcggcgg
     4321 ctggcgcgaa tgcctcgacc gcctcgacgc cctcgcccgc cagctcgtgc aacagcaggc
     4381 gcagcagcaa tgaggcccgc gtccgcccct gacccggagt cccgcccatg accgccccgc
     4441 tcaccggcga gagcgccgaa tttcacatcg aacgcctgat cgacgccccc cgcgagcagg
     4501 tctggcaggc ccacacccag gccgaacacc tggcccgctg gtgggggccg aaggggctgg
     4561 gcctgcgcgt cctgtccctc gacctgcgcc ccggcgggcg cttccactac gccatggtgg
     4621 cgcccgacag cagcgagacc tacggtcgct tcgtctatcg ggagatcctg gcgccggcgc
     4681 gcctggtgtg gatcaacagc tttgccgacg ccgccggcga ggccgtgcgt caccccatgg
     4741 cgcccgactg gccgctgcac atgcgcgtgc aggtggacct gtcggaggcc gacggccgca
     4801 cgcgcctggt gctgcggtcc cgcaccctcg acgccagcgc cgccgagtgc gccaccttcg
     4861 aggccggctt cgactccatg cgcggcggct tcggcggcac ctacgaccag ctcgacgccc
     4921 atctcgccac cttcaaccgg tcctgacgga ccttttccgg gagcccccca tgtacgccaa
     4981 tgcctacctg ttcttcgaag gccgctgcga ggaagccttg cagttctatc aggacgccct
     5041 cggcgcccgc gtcgagatgc tcctgcgcaa ccgggacaac ccccagccgc ctccgccggg
     5101 cacgatgccg ccgggcaccg aggacaagat catgcatgcc gcgctgcgca tcggcgacac
     5161 cctggtgatg gcctccgacg gctactgtca cggcggcgcg agcttccagg gcttcgccct
     5221 gtcggtgtcg ccggacagcc cggccgaggc cgagcgggtc ttcgcggccc tcggtgtggg
     5281 cggcaccgtg cgcatgcccc tgggcccgac cttctggtcg ccggccttcg gcatggtcca
     5341 cgaccgtttc ggcgtgctct ggatggtgaa cgtgatgccc gccggctgaa ggcgtcagcg
     5401 caccagcggg gcggacaggg ccagcgtcac cgcatcgtcc acgctcaggt cgctgagggc
     5461 gtccggcggc agtccgggca gggcggcgcg gcgcagcacc gtcagcgccg ccggcttgag
     5521 gcgggtgaag tacaggccca cgcccgcgcg ccgggtcgcc gcggcgagct cctccagcgc
     5581 ctccacgctg gtgctgtcca ggtccggcga ctcctccagg ctgacgatca gcgcgtggag
     5641 tccggcgtgg ccttccagcg tgcggcggat gccggtcacc acgtggccgg cgttggcgaa
     5701 gaagaggggg gtctccggac gcaggatgag gatgccgggc agcgcctcgg cctcgggatg
     5761 gagcttgagg gcgacgaagt cgtgcccgcc ggccaggcgc ccgagctccg acagggccgg
     5821 atcggacagc cgcttgaggg tcatcatcag gctcaccccg atggccgcca ggaggccgtc
     5881 cagcaccccc agcaggagca ccgccaccac cgccgccacc accacgatgc ggtcccggtg
     5941 cagccggaag cagggccgga agacgcccgg atcgagggag tgactcaccg cgaagacgac
     6001 gatggcggcc agcaccggct ccggcgtatc ggcgatgagg ggcagcaggg tcagcacgat
     6061 gagcagggcc accgccgcgc acacccaggt ggacaggcgg ctgcgggcac cggccgcctc
     6121 gttggccgag gttgcggaat agccggcccc caccggcagg ccgtggacca gcccggaggc
     6181 caggttggcg acgccgaggg cgaggagatc ccgattgacg tccaccgcgt cgccgtggcg
     6241 cagggcgaaa ttgcggatcg cgctgtagga ctcggcatag agcaccagca ccatggccac
     6301 cgccacctcg gcgaggcgca gccatgcggc gcggctcagg tcgggcagat ccggcgcagc
     6361 caggtgcagc cggatcgggc cgacggtgtc cacgccccag gcggcgagat ccacggtgcg
     6421 ggccaggacg atgccgagcg ccgtcaccac cagcgcgccg ggcaccgggc gcaggcgctc
     6481 cagcacgaag agcagggcca ggctcgcccc gcccaggccc agcccggcca ggttccagtc
     6541 cgccacatgc ccgaacaggg ccggcacgaa gtgcagcagg tcgccctggg cgggatgcac
     6601 atccaccatc ttgggcagct gcttgagggc gatggtgagg gccaggccga aggcgaagcc
     6661 gcgcagcacc ggcttggaga tgaaatccga ggcgttgccc atccgcgcca ggccggcgag
     6721 cacgaagaac accccggcga ggaggatcat gccgcccgcc agcgcccccc gcagcaccgc
     6781 gtcgcccccc gccaggctgg ccgtgaccgc cgccagcacc gccgccgacg aggaggtggc
     6841 ggacaccacc gcaaagcggc tggtgcccag ggcgccgtag gccagcaggc cggcaaagag
     6901 ggccatcacc ccggcctggg gcggcaggtt ggcgatgctg gaataggcca ccgcctcggg
     6961 cagcaggagg ccggccagcg agacgccggc gagcaggtcg ctgaagcggt cggagtcggg
     7021 cggcgcgggc gtggtcatga tcggcgggag tcgtaccgcc gggagtctac cccagcgcgc
     7081 cgcgtcaggc ccccgagcgc ggggcgtcca gccacttgag caccgtcgcg tagagggctt
     7141 ccggatcgac gggcttggcc acaaagtcgt ccatgcccgc attcaggcag cgcgcccggt
     7201 catcggcaaa ggcattggcg gtgagggcga tcaccggcag gccgaccagt tccggccggg
     7261 cgcgcaggcg ccgggtggtc tcgaggccgt ccagccgggg catctgcatg tccatcagga
     7321 tcagatccca gcgctctgcc gcgagccgct cgagggccgc gatgccgtct tccgcggtgc
     7381 tcacggcgag cccggcgtcc tgaagcagct ccgaggcgat ctcccggttc accggttcgt
     7441 cctccaccag cagcacacgt cgccccgcgt ggcgctggcg cagctgcgcc tcggcgccgc
     7501 tcaccgacgg accgcaggca gcgcccactg cggtcgccgc cttggccagg cgggccgaga
     7561 accaaaaggt gctgccccgc ccgggcgtgc tctccacgcc ggccgtgccg cccatcagct
     7621 cggccagctt gcgcgtgatg gtgaggccca gtccggtgcc accgtactgg cgggtgatcg
     7681 agctgtccgc ctggctgaag ggcaggaaca acgccggcag cacgtccggg gcgatgccga
     7741 tgcccgtgtc ctccacctcg aagcagatcc gcaggtggtc gccctcgtcg gccaccacgc
     7801 gcacgcgcag gacgatgcgt ccgcgctcgg tgaacttcac ggcgttggcg aggtagttga
     7861 gcaaggcctg ctgcaggcgc gccgcatccc cgcgcagggg cggaagcgcc ggcaccggct
     7921 cgcacacgag gctcaagccc ttggcctggg cccggtctgt cagcatcgtg agcacgttgc
     7981 cggtgaggcg gaccagatcc accccgccgt cctgcagagc gaacttgccg gcctcgatct
     8041 tggacaggtc gaggatggcg tcgatgatgt cgagcagatg gcgccccgcc gcatcgatct
     8101 tgtccaggcg ctccgcctgg gcgggcgtga gcagcgagcg gcgcagcaga taggccatgc
     8161 cggtgatggc attgagcggc gtgcggatct cgtgggacat gttggcgagg aactcgctct
     8221 tggcccggtt cgaggcctct gcggcggccc gcgcctgctc cagctgggcg gtgcgctggc
     8281 gcacctgctc ttccaggcgg tcgcggtggg catccagttc gcgggcgacg cgcacgcttt
     8341 cgctgatgtc cgaaaacacc accaccgtgc cgcacacctc gccgttctcg aggcgcggcg
     8401 tgaccgtcat gcgcaccgga aagccgctgc cgtcggatcg ccagaaccag tcgtcgtgtc
     8461 gtcgcggcat gccgtcgcgg gcggtctggt gggccgcgca ttcggcgtgg ggataggggc
     8521 ggccgtccgg gtaatggtgg tggatcaggg cgtgggtgtc ccgccccaga aggtccccct
     8581 ccgtgaggcc cagcagcttc agggcggccg ggttcgcgaa ggagcacgca ccgtcggcgt
     8641 cgatgccgta caccccctcc cccagggcat cgaggatcag gcgctgttcg gcctcggcct
     8701 cggtgaggcg ttgcagcgcc tcgtcggtgc gcaggcgctc ggcccgcagg cggccgttgg
     8761 cgcggaccag cagcagaacc gccgccgcca gcaccgtcat gcccgccgca agcagggata
     8821 caaggagcac gctgtagtgg tcccacacat ccaccagggt gaattcgggc acctgatcgt
     8881 agggcgccac ccgcagccgg cgcatcagat cctccaccgg ccggtaatcg ccggggatgg
     8941 tgaagccgtg caggccgatg cggttcgccg tctcgccacc atggggcagc ccgagcatcg
     9001 tcgcggccat gcgccgggcc agatcctcgt cggtccacgg catggccgcc accggccatt
     9061 ggggatagag gggggtagag gtctggaagg gataggtctc gtccgccaca gggtcgagga
     9121 cgcgcaactt cgcgccatcc agggagccgt cgcggacctt ggcctcgatc acgccgctcc
     9181 gcacgaaggc cgcatcggcg cgttcggcca gcactgccgc gagggccgta tcgtgggggt
     9241 agccggtctc ggtcaccgtc gcgtcctggg gcaggcggat gccgcgccgc tggagcatgc
     9301 cggcctgcat caggtagccg cccagggagc tgcgtgccgc cgtgaccagc cgcttgccgc
     9361 gcagatcgtc cacgctgcgc agccgcgcat cgtcggcgcg gaccacgatc accccgccga
     9421 atcgggcgag cggatggccg gcctcgttct ccaccagggt cgccaggggc gacagcagac
     9481 cttcccgcgt gctcagggcc acgtagtggg cgggctgggt cagcaccaca tccacctgcc
     9541 gcgcccgcac ggcggcgtca atctcggtgt cggtcatcac cagcagctcg aagcgccgcc
     9601 cgagcccggc ggcgttgagg taatcgatca ggggcatcca gcgcgcccgc gtctcgtcct
     9661 tggggcggaa ggccagcacg gccacccgcg ccggcacgtc ggcctgtgcc tcgtccccgg
     9721 cggcggccac cggcgcaccc cacgcgaacg cagccagcag ccccagcacc agcccccaca
     9781 cgacagcccg cgaatggacg aaacccggca ccttcccctc cccgccgcac gctggcagcc
     9841 tcaaaaaccc atgacccaca tagggatata tcggcacgcc gacagaaaac tcaacgcccc
     9901 cgccctgtca cacgaacaca ccgggaccgt caccaaggga tcacctgtcc gtcacaccgt
     9961 tgcagcatgc cgccgcaaca ctggcggcca tggatcgcgc cggactcgtt tgccaggaac
    10021 tcgtcagcca acagacctcc ctccgggtgg ctgtggtcac ggagacctat ccgcccgaaa
    10081 tcaacggggt ggccatgacc accggccgga tggtggatgg gctcgtcgcc gccggtcacc
    10141 gggtggatct gatccgcccg cgccaggccc acgaaggcgg cgtcgtgctc gacgcggatg
    10201 gcaccgacgg cgtcgaggag atgctctccc gcggcattcc catcccccgc tatgccaacc
    10261 tgcaactggg cctgccggcg cgcaaggtgc tgctgcggcg ctggtccgtg cagcgcccgg
    10321 acgtggtcca ggtggtcacc gagggccccc tcggctggtc ggccctggcc gccgcgcgca
    10381 agctgcacct gccggtgatc agcgaattcc acaccaactt ccaccgctac agcggccact
    10441 acggcatcgg ctggctcaag cgccccatcg ccgcctacct gcgcaagttc cacaaccgcg
    10501 ccgacctcac cctggtgccc acccgcgccc tgcgtgacga actggccggc cacggcatcc
    10561 cgcgggtgga cgtggtctcc cgcggcgtgg acaccctcct cttcgacccg gcccggcgca
    10621 gcgccgagct gcgccgcagc tggggcgtcg gtccggacga tctggtggtg ctctacgtgg
    10681 gccgcatcgc ccccgaaaag aatctcgccc tgctggagca ggcctacgtc gccctcaagg
    10741 cccgcgtacc cgccgcccgc ctcgtgatgg tgggcagcgg cccggcccag gcgggcatgc
    10801 gcgggcgcct gcccgacgtg atcttcgccg gcccccaggt gggcgagcag ctcgccgccc
    10861 acttcgccag cggtgacctg ttcctcttcc cgagcctcac cgagacctac ggcaacgtgg
    10921 tcgccgaagc cctcgccagc ggcctgccgg tggtggccta ccgggacgcc gcggccctcg
    10981 agctgatcac ccccggcgtg catggccatc tggccgaacc cggtgacgag cgcggcttcg
    11041 tgcacggcgc cgtccagctc gccgaatcgc ccgaactgcg cgcccgcatg cgcctggccg
    11101 cccgcgagcg ggtcgccagc ctggcctggc gggccatcgt ggacagcctg gtcagcctgc
    11161 agcggcaggt gatcctctcc tcgcccctct cccaccggag gtgatgccat gttcgccctg
    11221 cccgcccgcc tgcaactgat cgacgaccgc ctcgcggtcc gctccaaccg cctgagccgt
    11281 taccgcggcc tgcgggattt tctgcgcgtg gtgagccgcc tcggcgacgg cgtggcctgg
    11341 tacaccctgg ccgccgccct ctacggcctg ttcggcgccg aggccctgcc ggccctcacc
    11401 cagatgctgg ccgccggcgt cagcggcgtc atcgtctaca agctgctcaa gaaccacacc
    11461 ctgcgcccgc gccccttcga ggtggtgagc gacgtggtgt gcgccggctc gccgctggac
    11521 cgcttcagct tcccctccgg ccacaccctg cacgcggtga gcttctccct gatcctcgcc
    11581 caccacttcc cgcccctggc cggcgcagtc ttcggctttg ccctgctggt ggcgatctcc
    11641 cgcccggtgc tgggcctgca ctatcccagc gacgtgctgg ccggcggcgc gatcggcgcc
    11701 agcctcgcca gcgccaccct gaccctgctg taaatcccgg cccgggcggc ggacctgcgc
    11761 cacgcgcggg gcacggcccc tgcgccggcc gcattacatc ccgcgcggcc tgtcggtcct
    11821 cggcccgtgc ccatgcggat cacccgccgc gggcgtcgcg atgtccgtgg ccgtcatcgt
    11881 gcctctcccg cccccggccg cgccctgcaa cccggcgccc attgctatcc tgaaggggac
    11941 gaccgcccag cccccgccat gccctccacc gacaccaccg cccacccagc gtccatcctg
    12001 gtccgcggcc agatcctgca cttcctcgcc gatcccggcc cggaagatga ccccgccgcc
    12061 tgggaatact tcgacgacgg cgccctgtgg atcgtggatg gccgcatcca cgccgccggc
    12121 ccgtgggaag ccctccacgc cgccctgcca ccggcccgcc gcgccgccgc cacctactac
    12181 gactaccgcg gccgcctggt cctgcccggc ctggtcgaca cccacctgca ctacgcccag
    12241 gccggcgccc tcggcagctt cgggcgcgac ctgctggact ggctcggcgg cgccatcttc
    12301 ccggcggagg ccagctttgc cgaccccggc gtcgccgagc gcaccgcagc cttcgtcgcc
    12361 gaccgcctgc tcgcccacgg caccaccacc gcctcggtct tcgccaccgt ccacgcccac
    12421 tcggtggatg ccttcttcgc cgccgccgaa gcccgccaga tgcgcgtgct gtgcggcaag
    12481 gtgctgatgg accggcactg ccccgacgcc ctgcgcgaca cggccgcctc cggcgctgcc
    12541 gacagcctcg ccctcatcga acgctggcat ggccgcggac gcctgcgcta cagcctgagc
    12601 ccacgcttcg cccccacctc cacgccggaa cagctgcagc gcaccggcga actcttcgcc
    12661 gcctggcccg acctgcacct gcagagccac ctggcggaaa gccacggcga gcgtgcctgg
    12721 atcgcccgcc tcttccccgg ctgtcgcgac gatgccgagg tctacgcccg ctacggtctg
    12781 ctcggcccgc gcgccatcta cgcccacgcc ctccacctgg acgattccca gcgccgcgcc
    12841 ctggccgcca ccggcacggc cgtcgcgttc tgtcccagcg ccaacctctt cctgggcagc
    12901 ggcttcttcg actacgccgc cagcacggcc gccggcctgc gcgtcggcct cgccagcgac
    12961 gtgggcgccg gcaccagctt cagcctgatc cgcagcctcg gccgcgcctt cgatgtgagc
    13021 caggccaccg gcgcgcccct gtcggccctg cgcggctggt ttctcgccac cctgggcggc
    13081 gcgcaggcgc tctccctgga cgactgcatc ggcagcttcc agcccggccg cgaggccgat
    13141 ttcgtggtac tcgatctggc cgccacgccg gagctggcgt ggcgcatggg acaggcgcgc
    13201 agcctcacgg accagctgtt cgcgctgatg atcctgggtg acgagcgctg cgtcgtcgcc
    13261 acccatctgc tcggccgccg gcgctgggcg cggccctgaa cgacccgccg cggcgggtgc
    13321 cgggcagccg tcaggcgatg cggaagcgtc cggtcaggcc ctgcagctcc tcggccagac
    13381 ccgccatgcg gcgcgaggtc tcgcgcatct gcgaagccgt cgcggcattg gcctccgaca
    13441 cgctggcgat ctgctccacg cgcgaggcga tctcccgcgc ggccacggac tgctcgccca
    13501 ggccgagctt gatctcctcc atcgccgcca gcaccgccac cgagctgccc gagatcgtcg
    13561 tcaccgactc cccggcggag cgggcccgct cggccacgct ggcgaccccg cccacattcg
    13621 cgtccatgtc cgccacggcc ttcgacgtcc cgtcgatgac cttctccacc atcgtggtga
    13681 tctcccgcgt cgagtttccg gtgcgctccg ccagcttgcg cacctcgtcg gccaccaccg
    13741 caaagccgcg cccggcctcc ccggcgcggg ccgcctcgat ggcggcgttg agggccagca
    13801 gattggtctg gtcggcgatg ccgctcacca catccaccac cttggtgatg tccgcggcaa
    13861 acgcttccag cgcctgcatg gtgcccacca cctcctgcgt ggacgccgac atgcgcgaga
    13921 tctcgtccga cacgctcacc atgatctgcc cgccctccac ggccccgtcg cgggcgtcgg
    13981 cggacaggcc gtgggcgcgg atggtgttgt cgttgatgtg gtcgatggac accgacagct
    14041 cctcgatcgc cgccgccagg gcgccggcgt aggacgactg ctcctccgcc cgcgtggcgc
    14101 tcacctgggc cgactcgcgc atctgcacca gactcccctt cagattggcc gcttcctggc
    14161 gcaaggctgc ggcaatctcg tgcaggttgt tgcgcatcag cgccaggcgc gccacgagga
    14221 tgccaatctc gtcttctcca gcctggggca gctcgcacag cagattgccg cgggcgatct
    14281 catccgcggc gcgtcccact gccgccaggg gcttcaccac cgagcggatc gccagggcgg
    14341 aaaagaccac cgccaccgcc agcgccagcg ccgtcgcacc gcccaggcgg gtgcgcgcct
    14401 ccgtcacgaa atcgcgggac tcgccaacca gcgcgtcatt ccagcggttg gtcccgttca
    14461 ccaccgcgtc gatggccttg cgctgggcac cgtagagtcc ctgcaaggtg ttcagggcct
    14521 cctgcgcgcg ggccgcgttg ccgtcccgca gcgcggcgta gtaatccccg gtggcgaggg
    14581 catagaacgg cgccaccggc gcgtcggcct cctcgaggag cgccttgcgc agctcgggcg
    14641 gcaggtccgc cgcctgccac acggcgtgac gttcgtcgta ttcctgcttc agcttgagca
    14701 gacggctccg cgcggcgtcc agttcggcgc cctgctgatc gcgcatctcc cgcgccacca
    14761 gatagctttc aatgatgtag gccggcggcg gcaggatgtc ggcgagcagg tccttgccct
    14821 gcacgatgcg cccgaaacgg tcgccatcca cgccgctgga atggatgaca ccgctcgccc
    14881 accaggcggt cagggccagc agcaggagca gcaccgcgtt gccgctcatg acccgcgccc
    14941 ccaccgagcg gcgccagggc cgcatcagcc ggcgtgtcac gcgcagcagc ggaccggtcc
    15001 gggcgacctg cccttcatgc aaccggacgc tccgatcctt gcgcatgcgc gcgtagagcg
    15061 cctcggccgc ctgcacgtcg acccgcgacg gcgccacccg caccgacatg tagcccgccc
    15121 cgtccatcag cggcgtgacg ttggcgcgta cccagtagaa accaccgttc ttgcgccggt
    15181 tcttgacaac gcccgaccac gggcggccgc gcttcagcgt cgcccacatg tcacggaagg
    15241 cctcctccgg catgtccgga tggcggacca tgttgtgggg ctgaccgatc agctccatgc
    15301 gttcgaagcc gctcgcccgc acgaactcct cgttgcaatc gatgatggtg ccctgaatgt
    15361 cggtgcggct gatgatcgcc gcgcccggcg gcaggtgata ttcgtccggc gtgaccggca
    15421 ggttcgcgcg catgatcgtg tccgtcctct cgcgtttcgg tttcggtgaa ccgtcttccg
    15481 gaccgggtcc ggccgcctcc gtcgcgcctg cgctgcgcgc acgccgcacc ctttcctgtc
    15541 cgacatcaat gacaactgat gcgctatcga cgttggaaac gccgccttga gagcacgttt
    15601 tgcatttttt tcatttcgga aggcagcact gtcaggccca cccccgatgc gcgtcagccc
    15661 gcccccgcca gcctcgccag cacggtctgc aggcgggcca tctccgcgtc cgacagcgcc
    15721 gcgcgcagcc acgcgcccgt cgccgccacc acctcggcca tcgccgcctc gatctgcgcc
    15781 gcgcccgccg gcgtcagcgc tgccggcttg acccgcgcgt cgtctgccgc caggcggcgc
    15841 agcaccagcc cgcgcccctc caggtgcagc atcaccttgg tgagcccgcc ggaggtgatc
    15901 accacctctg cctgcagttg ggagggcgtc agctcgtggg gcggtggggc attgcgcaaa
    15961 gtcgccagca cgtcgaactc cgccagggac aagtcgtagc gggccaggac cgggcgcatc
    16021 gtctccagcg agcgggcgcg cgcgccctgc agggccagga agaggggcac gacccgggaa
    16081 tcgagactgt gatcccagcg cggagaatcc tgatcggcaa cgacgtccat gggtgtcctc
    16141 gggaaacatc cccgttatgt aaaggcaggc aaatcatctt gctggaaaga tatctgcccg
    16201 atagattgga ctcaagtccc attcagatcc aatccaccat gaaagccatc cagcgcaccc
    16261 tcgccggctt cctgctcctg ctcaccgccg gctgggccct ctccgagccc ggcgacctcg
    16321 tggccatcgc cagcttcttc gacttccgca gcctcttcat gcaatggagc ggcgtgatcg
    16381 ccatcggcgt catgagcctc gccatgatcc tgtccctgcg ccccgccgtc ctcgaaccct
    16441 acctcggcgg gctggacaag ctctaccgcc tgcacaaatg gctgggcatc accgccctgg
    16501 gcatcaccct cgcccactgg ctgatggcca agtcccccaa gtggctgatc gccctcggct
    16561 ggctcgaacg ccgcggccgc cgcccgcgcc cgagctttcc cgaaggctcc ctgcagggcc
    16621 tcttcgccgg cctccacggc ctcgccgaag acgtgggcga atgggccttc tacggcgccc
    16681 tggtgctgat gctcatcgcc ctcatcaagg cctttcccta ccgccgcttc gtgcagaccc
    16741 accgcctgct gcccctcgcc tatctggcgc tggtcttcca cgccgtcatc ctgctcaagt
    16801 tcgacgcctg gcgcggcccc atcggccccc tgctggtcgc gttgatggcc ctcggcagcg
    16861 tcgccgccgt gtccagcctg ctcggctggc gccccggcag gcgccctgtc accggcaccg
    16921 tcacggccgt ggccgccgac gccatcgatc cggtcctcac cgtcgacctc cggctcgacc
    16981 ccggctggcc cggccacacc gccggccagt tcgccttcgt caccttcgac agccgcgagg
    17041 gcgcccaccc cttcaccctc gcctccgcct ggcgcggcga cggctacatc cagttccgca
    17101 tcaaggccct cggcgactac acccgcacgc tggccgcccg tctccacccc ggcgcccgcg
    17161 tcagcctgga aggcccctac ggccgcttca ccttcggcgg caacgccgcg cgtcagatct
    17221 ggatcgccgg cggcatcggc atcaccccct tcctggcccg cctgcaggcc ctcgccgcca
    17281 cccccgacgg gcgccgcacg gatctcttcc actgcaccgc gcgctattcc gccgacgcgg
    17341 tccgtcagct ccgccacgcc gccgacgccg ccggcgtgcg cctgcacatg ctgtgggacg
    17401 aacgggacgg ccgcctcgac gccgcccgcc tcgccgagac cgtgcccgac tggcgcgacg
    17461 ccgacatctg gttctgcggt ccggcgccct tcggcgacgc cctgcgcacg ggcctcgtcc
    17521 gcctcggcct gcccgcccgg cgcttccacc aggaactctt cgagatgcgc tgagcacgcg
    17581 gcgcggcgcg cgcgtcccct gccgcgccag ggcccggacg cccgcccgct gcgctagaat
    17641 ccaccccggg tgtccctccg cgccgtgcgg acggacaggt ttcgtgacgg tcgggccgcc
    17701 tcacgggaac atcccatcat ggccctccgc ccactgcgca gacgcctgcg cgcccgcctg
    17761 tcccccgcca cgacggcacc gccgtgtccc gccgttgctg ccttcgcggc cccccacgca
    17821 cgcttccccg caccgccgcg acgccctgac gcgccgcgct gcgccattcg gcgcctcccc
    17881 gtctgaccgg aatgccccca tgctcaccgt cctcatcctg atcttcgcgg tcacctacct
    17941 ggccatcgcc ctcgagcacc ccctcaacct ccacaaatcc gcctcagccc tggccggcgc
    18001 cggtctgatg tggaccatct acgccctgct cgggccggac gccgccaccg tcgcccacca
    18061 gcttgacgac accgtcaccg ccacggcgca gatcgtcttc ttcctgatgg gcgccatggc
    18121 catcgtcgag gtcattgacg cccaccacgg cttcgacgtc ctcacccgcc gcatccgcac
    18181 cacccagcag accaccctgc tgtggctcgt cggcagcgcc accttcgtgc tgagcgccgt
    18241 gctcgacaac ctcaccacca ccatcgtgat gatctccctg ctgcgcacgc tgctggcccg
    18301 ccacgaggac cgcctcgtct ttgccggcct cgtggtcatc gccgccaatg ccggcggggc
    18361 gtggtccccc atcggcgacg tcaccaccac catgctgtgg atcggcggcc agctcagcgc
    18421 cgccgccatc atccgctcgg ccctgctgcc gtccctcgtg tgcacgctgg tgcccctcct
    18481 ggtcctcagc ctcgccctgc gcggccggcc cgtcgtcgcg ccggacgcgc cggaccccgc
    18541 caccgccggg cccggcgcaa ccgagtttga gcgccgcttc atgttcgccc tgggtatcgg
    18601 cgtgctcgtc gcggtccccg ccttcaagac cctgacccac ctcccgccct tcctgggcat
    18661 cctcttcggg ctgggcatcg tgtggctggc gggcgacctg atccaccggg acaagtccgc
    18721 cgagtaccgc gaacgcctga ccctcgccca cgccctcggc cgcatcgacc tgggctccgt
    18781 cgtgttcttc gtcggcatcc tgctggccgt cgccacgctg gaacacaccc acctgctgtc
    18841 cgccctcgcc gcctggctgg acctgcgggt gggccgcgaa gacctcatcg tgctgctcat
    18901 cggcatggcc agcgcggtgg tggacaatgt gcccatggtg gccggcgcga tgggcatgta
    18961 tcccctcgcc gactaccctg cggatcactt cctgtggcgc ttcctggcct attgcgcagg
    19021 caccggcggc tccgtgctga tcatcggctc ggcggcgggc gtcgccgcca tgggcatggc
    19081 ccacatcccc ttcggctggt acctgcggcg catcagcggg ctcgccctgc tcggctacct
    19141 ggcgggcgcc ggggtgttcg tgctgcagca gggcgtggcg gcctgaccca cgccggtcca
    19201 cggccgcccg gcgcgcgccg gcacggcgtc aatcgccggc cgccggtgcg gcggcagccg
    19261 tgaggtagtc cagggccacg ttggtcaggg tgcgcacgcc gatggcgagc acccgctcgt
    19321 cgatccagaa gcgcggcgaa tgattgggga acaccgtctc cggccgcgcg tccggcggca
    19381 ccaccccgag gaagaagaag acccccggca ccagcttctg gtacgcggag aaatcctccg
    19441 aaacggtgac cttgggattc acgaacacct gatcgcgccc cagcgtggcc cgcagggact
    19501 ccgccgtcag gcgggccaga cgctcatcgt tcaccgtcac gtcgtagttc ggcgtgatcg
    19561 tcaccgccgc gctgccgccg ccgctctccg cgatcttctc ggccgtgcgg atcaggcgct
    19621 tccggatgtc gccgcgcatg gtctcgtcaa gggcgcggat catgccgctc agctccaccg
    19681 tgtcggggat gatgttcggc cggttgcctc cgttgatcgc gccgacggtg atcaccgacg
    19741 gttcgagcag cacattcacc tggcggctct cgatggtctg caggcccatg accacctgcg
    19801 ccgccagcac cacgggatcg acgcccagcc acggcagggc cgcgtgggtc tggctgccct
    19861 gcaccacgat ccggaaagta tcgccgctcg ccatgaaagg cccgccgcgc acgcccgcct
    19921 tgcccagggg cgtccggctg ttgaggtgca ggccgaagat cgccgagggc gccggatcct
    19981 tcaacgcccc ttcgcggatc atcagcgcgg ccccgccctc ctcgtcgggc ggcgggcctt
    20041 cctcggcggg ctggaagagg aacaccaccg tgcccggcac ccgctcccgc agcgacgcga
    20101 gcacctgcgc cacgcccatc aggatcgccg tatggccgtc gtgaccgcac acatgggcga
    20161 cgcccacctc ctgcccctgc cagcgtgcct tctgcgtcga cgcaaaaggc aggccgctct
    20221 gctcggtgac cggcagggcg tccatgtcgg cccgcagcgc caccaccggc cccggcagac
    20281 cgccgcgcaa cacccccaca acgccggtgt gggccaccgg cgcacgcacc tccagcccca
    20341 gcgcgcgcag atgatcggcc accagcctgc ccgtccggaa ctcccgattg cccagctcgg
    20401 gatgctgatg gatgtcccgt cgccacgcga tcacctgcgc ctccagacgc tgcgcctgcg
    20461 cctccacctg cggccgcagg cttcccggct gctgcgcgag ggcattgccc agccccagga
    20521 caaggcccag cacccccacg ctcatgctcc gtcgcatcgt cgccgctccc gaatggatcc
    20581 gcccgccccg cgctgcgccg ccgcagcccc tctggatcat tctagacagg caccgccatt
    20641 tcgccggacg cctgcgcccg cggcccccgg cccgcgacgc agtctgcgcg ccccaccggt
    20701 cgcggccgca ctgtgcgcgg cgcctactcg atcagccccc gcgcccgcgc atccgcctcg
    20761 tccggcatga tcgccatcgc cgtgcgcatg cgccggaaac cgaactgcgc gtagaacgcc
    20821 tccttgcccg gcgcggcgta gagcatcgtc agctccacat tggcggcggc cagcagacgc
    20881 cgcacgatgg cctgcccgat gccgcgcccc tggtattcgg gcaacaccgc cacgtcgtac
    20941 agcgccgccc gcgacacccc gtccgacaag gcccgcccca cgccgatcag caccggcccg
    21001 gcatacacga agaggcacag cgcgctgttg cggaaggccg tctccaggcg ctcgggctca
    21061 cgccgccggc ccagcttcgc ccgctcgaag agcgccacca gcgccggcca gtccacccgc
    21121 tcgatgtccc acgaaaagtc cagccccgcg gccccgccag cgccgtcctg ccccagcgtc
    21181 gtgtctgcgg atgtcatgtg tcccgccctc acaccggcag cgccgacgac gccttcactt
    21241 cccgcaggga caggctcgaa cggatcgccg tcacccccgg cagcgtgcgc aggcggtcgc
    21301 gcacgaagag cccgtaggcg tccagatcct ccgccaccac ctgcaacagg taatcgcagt
    21361 cgcccgtcac gttgtgacag gacaggatct gcggcagccc ggccacctgc cgctcgaact
    21421 gcaggggcgc ctccccggcg tgatccccca gcgtcacctg cacgaaggcc agcaccccgt
    21481 agcccagctt gcgccggctg agccgcgcct gatagccctc gatgaagccc tccgcctcca
    21541 gccgacgcag gcgtcgccag cacggcgtct cgctcagcga cagggtctcg gccagacgcg
    21601 cgttggacat gcgcccgtcc ccctggagca ggcgcaacac cgtgcgatcc acctcatcca
    21661 gcccatgtcc ggaaagaatc attccctgcc tcctcgatat caggatgaaa ctgtccgaac
    21721 agtggctgtt cacgaaggat ttggcaatcc tcttcccctt cattggcccg atcatgaagc
    21781 ttccgcaccg cccgacgaag cccaccgccg tgatcgacac cgccgccctg cccctcttcc
    21841 tcgccgccct cgccgccgcc tacctgctgc cggggccgga catggccctc atcgtgtcca
    21901 ccaccgcccg ccacggcccg cgcagtggcc tgatggccgc cgccggcctg gccctgtccc
    21961 gcagcctgca tgtgctcctc tccggattcg ggctggccgc cctcttcgcc gcccatcccc
    22021 tgctcttcca cggcgtcaag ctgctcggcg ccgcctatct gctcggcctg gcgtggacac
    22081 tcctcaccgc ccccgacacc acgcccggcg cgaccgccgc agccgcctgc gcatcgccgc
    22141 ccggcgcggc agacgccctg cgccgcggat tcctcaccaa tctgctcaac cccaaggccc
    22201 tcggcttctg cgccatcctg ctgccccagt tcgtcgcgcc ccaccagccc ctgcccggcc
    22261 agttcgcggt gctcgggctc atcctcgtgg ggcttggcgc ggccttcgac gcggcctaca
    22321 tctgtgccgc ccagggggtg gcccgccgct ttgccgcggc accgcgcctg cgcgggctgt
    22381 cgcgcctcac ctgcgccggc gtcttcggcc tggccgcgct gcgcctcgcc ttcagcggca
    22441 ccgactgaag cctcccaccg cgccccgcgc cggccaccgc cgccccgcct cagcccatca
    22501 accagcgccc gacgcccagc cacagggccg tgacgcccag catcgccgca aaggccgtgc
    22561 gcatgcgccg ctcgggcagg cgatgggccc acgccacgcc cgccgacacc gtcgccagcc
    22621 cgcccacggc catgggcagg ccccgcgacc agtccacatg ccccgccgac gcatacgccg
    22681 ccagcgccac cgccgagctg ggcgccacca gggccagcga caagccctgc gccaccgcct
    22741 gccgttgccc cagccagccg gcgagcaaag gcgtcgccag caaaccgccg ccgatgccca
    22801 gcaaacccat gctgcacccg cccgccgccc ccacaacggg cagccagcgc agggccaggg
    22861 gcttctcggc ccccgccgcg gccggcgcgg cgcgccacag gcggatcgcc aaggccagca
    22921 ggaacacgca gaacaccccg cgcagcacca gcgggtccaa ccgcgtcgcg aaatgggcca
    22981 tgccccaggt gaccagcgtc gccgagccgg caatggccgc ctgggcccgc cacggcatgg
    23041 gctggcgctg ctggtaacgc caccagccca ccagcagatt cggcaccatc agcaccaggg
    23101 ccgtcccctg ggcctgcgcc tgctccatgc caaagcccag cacgaacagg gggatcgcca
    23161 ggatgccccc gccgatgccg aagagccccc cgaagaagcc cagccccgcc cccaccacca
    23221 gggccagccc catgtgggcg tccactacaa agtccatgcg cgtgccccct tcaaaaaccg
    23281 atccgcgcac tttcattgat tcaccgcatc gctacaatcg tccgcactgc aattcatccc
    23341 tgcgtacacg gcacgaatga acgacctccc ggacctcgcc ttcttcgccc tcctcgcccg
    23401 ccagcccacc ctggccgccg cggcccaggc cctgggcgtc acgccctcca gcgtgagccg
    23461 tcgcctcgcc gcgctggaac accgcctcgg cgtgcgcctc ctccaccgca ccacgcgacg
    23521 catcagcctg agcgcggaag gcagccgcta cctggaaggc ggcgaacaga tcctgcacga
    23581 cctggacaac ctggagcgct ccctgctcgg cagccgcgaa atgccccgcg gcctgctgcg
    23641 catcaacgcc accttcggct tcggccgtcg ccacctggcc ccggcccttt ccgacttcgc
    23701 gcgacgctat cccgacgtgg aagtgatcct cgatctcacc gaccgccccc tcgacctgac
    23761 cgaacatgcc gtggacgtgg gcatccgctt cggccccccg cccgacgccc gcatccaggc
    23821 ccgccgcatc gcccccaacc gccgcctcgt atgcgccgcg cccgcctacc tggacgccca
    23881 cggccacccc gaatcccccc gcgacctgcc gcgccaccgc tgcatcgtca tccgcgaagc
    23941 caccgccgcc tacaaccact ggcagctcac cgacggacac gaacagatca tggtcaaggt
    24001 cggcggcccc ctctccacca acttcggcga gatcgccgtc gaatgggccc tcgccggcca
    24061 cggcatcgtg ctgcgctccg aatgggacgt ggccgccgac ctccgcgacg gccgcctgca
    24121 acgcctcctc agcccctggg ccggcacgtc cgccgacatc cacgcggtct atccgccgcg
    24181 gca
//
LOCUS       DAOQPA010000044        23854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1004_length_23854_cov_18.790201, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000044 DAOQPA010000000
VERSION     DAOQPA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23854
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1004_length_23854_cov_18.790201"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKX01_08080"
     CDS             complement(<1..1045)
                     /locus_tag="PKX01_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMZ76121.1"
                     /translation="MSESHAPRFVHLRLHTEYSISDGIVQVDAAIARAAADGMPALGI
                     SDLANLFGMVKFYKGARGKGIKPVVGADCWITHDADRDKPARVLLICRNRAGYGQLCE
                     LLTRAFMENKYRGRAEIRREWLTADTASDLLCLSGARDGDIGQALAAGNLALAERLAA
                     DWAARFPDAFYIELQRAGHPGTEAYIRDAVGLASRLALPVVATHPVQFTRPEDFKAHE
                     ARVCIAQGTVLADKRRARDFTEAQYFKTQDEMCALFADLPEALANSVEIARRCSLTVQ
                     LGKNFLPLFPTPDGMSLDDFLILEAKKGLEERLVQLYPHPDERAAQRQRYEDRLKFET
                     DTIIQMGFPGYFLI"
     gene            complement(1120..2241)
                     /locus_tag="PKX01_08085"
     CDS             complement(1120..2241)
                     /locus_tag="PKX01_08085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ76122.1"
                     /translation="MNHDTFLNVLFSRVPRADGTRALVVLNVLVFLLTALAAGSLMQF
                     PSDLLIRLGGNFAPLVQKGEAWRLATALFLHGGLLHVSLNMLALHQVGQVVERLYGRS
                     GFLVIYVGAGLLGNLASLWWKQSGVSVGASGAIFGVYGALLAYLTAQRGSVPVQIFAE
                     MRSGTLGFIGYSLFAGFAVPGIDNAAHVGGLLGGLLLGATLARPLVEPDPVRWQSPLA
                     IGGLVVALVLGGVLWIRTPQVVQRFEMNTLFQQNAQQFSVEEQDLLRQQALLMDNLRH
                     RRVTAVQALDTLDNDLIPRWDRQIGVLSDHLAAPEQDWQRVELIHYATARRDALKALA
                     QAISTRQAAWVQQANDLEQQANNILLQMRLRNSLEASRR"
     gene            2408..3448
                     /locus_tag="PKX01_08090"
     CDS             2408..3448
                     /locus_tag="PKX01_08090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ76123.1"
                     /translation="MIHILKHVESITQQRDRPLLELGVASALFEVVRAREVNLFKVIY
                     GQGMPRVERVTHVGQDGVRYVDTDFVDTIETEVIDARAEMAACVMDRQIVRRHDLDRG
                     VYVHCFPVDVDRRVLGVLELVTPHVLDDEQMGTAEGFLGLYRNYLNILDYSERDTLTG
                     MLNRKTFDDNLGKLLAGLTPSGGNGSSLPARRAVECGRHWLAVIDVDHFKRINDHYGH
                     LYGDEVLLLLARIMQQSFRHQDKLFRFGGEEFVVVLRSSGPGETATALERFRTAVDTH
                     VFPQIGHVTVSIGYTPIRANDTIPDIVGRADEALYFAKDHGRNQTVSHDDLLAKGLIA
                     PRERPQGDVELF"
     gene            complement(3509..3859)
                     /locus_tag="PKX01_08095"
     CDS             complement(3509..3859)
                     /locus_tag="PKX01_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76124.1"
                     /translation="MAWALMWALSVPAGAQLPPSTALLNPVQSAWLQAEHRKVEESFV
                     QKVMGITGASREQVMRAIPARGRITDRLSRIYSALERDLGAPLSDEQKGLIYAAEGDR
                     KQARKDAESAATSR"
     gene            complement(3895..4869)
                     /locus_tag="PKX01_08100"
     CDS             complement(3895..4869)
                     /locus_tag="PKX01_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HMZ76125.1"
                     /translation="MSHQIRFSRTGGPEVLEWCESEPLPPGEGEARVRHHAVGLNYID
                     VYHRSGLYPVPLPSGLGLEAAGEVEAVGPGVTGLKPGDRVAYAGGPLGAYAEVRNLPA
                     DRLVILPDALSYEQGAAMMLQGLTAQYLLRRTYRVQSGDTILIHAAAGGVGLIVCQWA
                     KALGATVIGTVGSDDKAALARAHGCDHAIVYTREKVSERVRALTGGQGVQVVYDSIGR
                     DTFMDSLACLAPLGMMVSFGNASGPVDAFDPGVLARMGSLFLTRPSLFHYAARREQLV
                     AMADELFAVVTSGQVRLSIGQRYALQDVAAAHRALEARRTTGSTVLIP"
     gene            complement(4967..7258)
                     /locus_tag="PKX01_08105"
     CDS             complement(4967..7258)
                     /locus_tag="PKX01_08105"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMZ76126.1"
                     /translation="MDKDFIAAALEYHRSPTRGKIAVVPTKGLTNQRDLALAYSPGVA
                     AACDAIVADPADAQEYTSRGNLVAVVTNGTAVLGLGNIGPLAAKPVMEGKGCLFKKFA
                     NIDVFDIELAENDPDKLIDIIAALEPTLGGINLEDIKAPECFYIEKKLRERMNIPVFH
                     DDQHGTAIISAAAMLNGLKVVGKDIGAVKVVCSGAGAAAIACLDLWCSLGIRRENVFV
                     CDSKGVIYVGREENMEPTKARYAQNTAFRTLADAMVGADVFLGLSTAGVLKPEMVKTM
                     ADKPLIFALANPTPEIMPELAKEVRPDAIIATGRSDYPNQVNNVLCFPFIFRGALDVA
                     ATKITDEMKLACVKAIAELAEAEQSDVVAQAYGGADLSFGPNYLIPKPFDPRLIVKIA
                     PAVAQAAIESGVATRAIDMDAYVQSLTEFVYQSGIIMKPVFSKAKGIPADKKRVLYTE
                     GESERVLRAVRAVVDENLARPILIGRPAVIDMRIQKFGLNLQAGRDFDVVNVESDSRY
                     RELWHEYYRLMGRDGVTPDSAREALRSDTTLIGCMLLRRGDADALVCGTTGAYDHHFN
                     HVRNLVGLKKGASLFAAMNMLLLPKRVLAIADTYVNEMPGAEEIAEITVMAAEELKRF
                     GIEPKAALLSHSNFGSAPSASSRKMRAARDLLRVQAPDLQCDGEMHGDSALSAEIRDR
                     LHPDSTLTGEANLLVMPNLDAANIAFNLIKVANGDGVSVGPILLGAALPVHILTPSAS
                     VRRLVNITALAVVDAAEQRVEKV"
     gene            7599..8366
                     /locus_tag="PKX01_08110"
     CDS             7599..8366
                     /locus_tag="PKX01_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ76127.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAISAHQGLRTLVVDLDPQGNST
                     HYLLGHALENADDTLAGLFEQTLAFKFNPRQTAEFVQPTPFPGLSLLPSHPSLEELQS
                     KLESRYKIYKLRDALDELADRFDAVYIDTPPALNFYTRSALIATRRCLIPFDCDDFSR
                     RALYALLENVQEIKADHNRDLEVEGIVVNQYQPRASLPQKLVQELIAEGLPIFKTYLS
                     SSIKIRESHEQAKPMIHLEPRHKLAQEFIALHGELKG"
     gene            complement(8425..10647)
                     /locus_tag="PKX01_08115"
     CDS             complement(8425..10647)
                     /locus_tag="PKX01_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ76128.1"
                     /translation="MSQLLEHLNAEQLAAVTLPPRHALILAGAGSGKTRVLTTRIAWL
                     IQNGHCSPQGILAVTFTNKAAKEMLTRLSALLPINTRGMWIGTFHGLCNRFLRAHHRD
                     AGLPQLFQILDSGDQLAAIKRLLKTLNVDDEKFPPRDLQHFINGQKEAGIRPPAVEAW
                     DDFTRRKVELYAEYEAQCQRESVVDFPELLLRCYEVLERNEPLRRHYQARFRHILVDE
                     FQDTNRLQYRWLSLLADGGREGGARVFCVGDDDQSIYRFRGAEIGNMRDFERAFHVTD
                     VIRLEQNYRSHGNILDAANALIAHNRERLGKHLWTDRGAGEPIRVYEAFTDTDEARWI
                     VEEIQSLVRDGVSRSSVALLYRSNAQSRVLEHQLFSNGVAYRVYGGLRFFERQEIKHA
                     LAYLRLLANPDDDTAFLRVVNFPTRGIGARSLEALQDAARTWNTPLYQTVPHLSGKPG
                     TALAQFVTLIERLRHETRDLPLHERVDHVLERSTLRDHYKGEKEGQERLENLDELINA
                     AANFSAEFQVNGASEGAPDTATVLAEFLAHASLEAGDHQADAGSEAVQLMTVHAAKGL
                     EFDVVFLSGLEEGLFPHENSILESEGLEEERRLMYVAVTRARQRLYLSFTQSRMLHGQ
                     VRYNLRSRFLEEIPDGLTKWLTPPNTRHGGFAAESPARYFTPSSARPAAAVMPVSPPL
                     KSRDLGGFAIGQNVRHARFGDGVIVAAEGSGADARVQVKFGAAGSKWLMLSMAKLTAA
                     "
     gene            10956..11282
                     /locus_tag="PKX01_08120"
     CDS             10956..11282
                     /locus_tag="PKX01_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76129.1"
                     /translation="MKSPKILPWYARKAGVSVERAEALWRKAVREATADTGWVGTSEF
                     WGAALDRFLELLQDEQTTLCQPHMETFVRSQHRMWLLPLLAAEDMFNAMSANWQRYRN
                     QMRKAA"
     gene            11480..11812
                     /gene="yajC"
                     /locus_tag="PKX01_08125"
     CDS             11480..11812
                     /gene="yajC"
                     /locus_tag="PKX01_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMZ76130.1"
                     /translation="MLISNAFAQAAPAAAQDPTAGYGQIVIMVAMFAIFWFMLIRPQQ
                     KRAKEHKALIEGLQKGDEVVTQGGLVGKVAQVGDNYVHLEIAANTQVVVQKPAIGIVL
                     PKGTLKSL"
     gene            11881..13740
                     /gene="secD"
                     /locus_tag="PKX01_08130"
     CDS             11881..13740
                     /gene="secD"
                     /locus_tag="PKX01_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HMZ76131.1"
                     /translation="MNRYPLWKNLLIGLVLLWGLLYTLPNFYGEVPAVQVSSGKATLK
                     LDATATLKRVEDTLHGAGLQHDGLFADLTSVRARFHDTDTQLKAKDAIEKAFNPDPAD
                     PSYVVALNLLSASPQWLTAIHALPMYLGLDLRGGVHFLLQVDMKGAVTKRLDATAGDL
                     RTLLRDKSVRHAGISREGQSVAIRFRDAETLARGRAVITASTNDLLLSEGQDGADFKL
                     TATLNPLAQKNIQESAIRQNIVTLHNRINELGVAEPVIQQQGGDRIVVQLPGVQDVAK
                     AKDILGRTATLEIRMVDDAPGALEAALNGSIPPGSELYSERGGSPLLVKKQVVLTGER
                     LTDAQPGFDGQTHEPAVHLTLDAAGARIFKDVTRENVNKRMAILLIEKGKGEVITAPV
                     IRGEIGGGRVQISGRMSSEEATDTALLLRAGSLAAPMEIVEERTIGPSLGADNIRKGF
                     HSTLWGFVAIAAFMCLYYMLFGLVSVLALGSNLLLLVALLSLLQATLTLPGIAAIALT
                     LGMAIDANVLINERIREELRNGSSPQAAISAGYERAFDTILDSNVTTLIAGIALLVFG
                     SGPVRGFAVVHCLGILTSMFSAILVSRGLVNLIYGKKRKLEKLAIGQVWKPGL"
     gene            13774..14706
                     /gene="secF"
                     /locus_tag="PKX01_08135"
     CDS             13774..14706
                     /gene="secF"
                     /locus_tag="PKX01_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HMZ76132.1"
                     /translation="MEFFRIHKDIPFMKHALVFNVISLITFLLAVFFLVTKGLHFSVE
                     FTGGTLMEVAYTEPPTLERLRDALAHEGYADAQVQNFGSARDVLIRLPNREGMETAKV
                     SEKVMATLAAVEGPKAELRRVEFVGPQVGKELASDGAMALLLVIGGIVLYLALRFEWR
                     FAVSAIVANLHDVVIILGFFALFQWEFSLPVLAATLAVLGYSVNESVVVFDRVRETFK
                     KKRGLTTPEVLDHAITSTISRTIITHGCTQIMVLSMLLFGGETLYYFALALTIGICFG
                     IYSSVLVASPLVMWLGVSREQFIQVKKDKQEAVV"
     gene            14767..15519
                     /locus_tag="PKX01_08140"
     CDS             14767..15519
                     /locus_tag="PKX01_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76133.1"
                     /translation="MPDPAPRLHDCLADASGGLRFHLRAWRHRRRWAAFHAAVADWLR
                     AWHPDRPHLVLVGPSAGYDLPAGWLGGFTRITVLEPDPLARWLLRRRPDATRLHFDDL
                     DCLATPDGLAHLKARFPDAAILFCNVLGQVTAPGGRGWAELCACHLRDLPWASLHDVV
                     STPRAPRADAVRASFPGGTSLEAVLAAFWPGGELPLVDHATFALGGDVPHHYALWPLR
                     PGRWHLVEWVAHAMQNDTSAMPDDAAGRGRAV"
     gene            15553..16188
                     /locus_tag="PKX01_08145"
     CDS             15553..16188
                     /locus_tag="PKX01_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76134.1"
                     /translation="MHVLRLACRLLLVTLLLAAGGARAQALNDTYEGLLLPDTFEQPI
                     PVTVKLTEMGTLLLGQVKTLAPLPGEAALSRAERNNARCLIFAPIAAATHLRMEGTCV
                     PGLFEGNFLISRNGRDTRGTFTLPRKAPAAPSADPADAPRRAAPVDAASPITACLKAN
                     TACLIACPRGDYNVEFLCSNRCRQKFQHCKGKSTLQTPAQPATGSTTPSAN"
     gene            16252..17356
                     /gene="prfB"
                     /locus_tag="PKX01_08150"
     CDS             join(16252..16332,16334..17356)
                     /gene="prfB"
                     /locus_tag="PKX01_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMZ76135.1"
                     /translation="MEAERLNALSNALADLKARELELRRYLDYDTKAEKLIEVSKQLE
                     DPEVWNDAPRAQELGKEKKTLEGVVLTLDEIDQGIADGRELFELASMEEDEETVQAVE
                     ADVAQLQGKIGELEFRRMFSNPMDANPCFIEIQAGAGGTEAQDWAGMLERMYLRYCER
                     KGFQVELMEESEGEVAGIKSCTIKVGGEYAYGFLRTETGIHRLVRKSPFDSNARRHTS
                     FTSVFVFPEVDDSIEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQC
                     QNDRSQHRNKDEAWSMLRARLYEAELRKRQSEQQKLEDAKSDIGWGHQIRSYVLDQSR
                     IKDLRTSYEVGNTQAVLDGDLDGFIQASLKQGV"
     gene            17369..18622
                     /locus_tag="PKX01_08155"
     CDS             17369..18622
                     /locus_tag="PKX01_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76136.1"
                     /translation="MVRLLAWLSGGLALLLVALAGLLALAVEDHPLVVADDALSPAAI
                     SRARELLRRHDPRKLVRGETRKVDLGADDLASLLRFATRRGLHGQSALRLEQGHAEVR
                     FTAPLPATPLGRYLNLTADLGVSDGRIDIDGVRIGRLPVPAPLFSALFDAYVRHTDLA
                     PELALLRRTVTRVAIAPNALSFVYVWQPELLDSARDLAIGPTRRQRLADAHLRLVARM
                     DAVAARGPRAPLADVLGPLLAGAGEAPAGGERSAAYRDALLVAAFHLSGKDVGAVIPE
                     ARQWPRPRPLTLTLRGREDLAQHFTVSAAVAAAGGEPLANAVGLDKEIDDSDHGSGFS
                     FVDLAADRAGTRLGELAVRNPERLAAALAAGLTDSALLPPIDGLPEAMMAAEFRQRFG
                     GPDAPAYRRMRDEIERRIAALALFR"
     gene            18635..19975
                     /locus_tag="PKX01_08160"
     CDS             18635..19975
                     /locus_tag="PKX01_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76137.1"
                     /translation="MSEVRLEELLAPGVVADDVPELDTLLRARPLLAAFSTLFHGSEA
                     EVLARLLVLREIGRDAQTTHWTPERLRQRFTYLDPVKLETVLRRLRGNGLLDIGDDNR
                     YTLSDLGRNAVAAVDMLVGFSAGDDLELGFLTAQLAGLQAVGSVTPEALGHLLGKLND
                     LAWHFEDAIASGSEFRIADARRRLSANTRWIEKGTEVLRTLLADPELPFDLARLAQPI
                     GLAQSRLARIDAAFQRAVNKIEAQRVTLGGSGISSSDVAAWLRGRDARALAALARNAL
                     AAAPDLTLLAGGHELLDRAELALSEDARRMEADTALPPPEEAPRRDAPEQEDLRLLEH
                     FSRRLARLAEPGQAPETLARAVAGGGFPAASYRLSLLALLADGGDSDTASPADGPIGD
                     FMRLPVDIRFELATEPVNEDEIATMSAGLVGARGRLPAAEAVPVLDPDTLPRPA"
     gene            19975..20676
                     /locus_tag="PKX01_08165"
     CDS             19975..20676
                     /locus_tag="PKX01_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76138.1"
                     /translation="MEHQIRTLTARLLAHRSLPRSDPLVRLALVDDAFRSDLDARLGA
                     VGLSLIDNPYAAHVAVALAEDMHEPVFGGGREYAASNAGLTRDQLALLVVIWSLIVLP
                     KRERQVNRQRLTDDGQEDMFGKEGPLAHGGEVSGALSEASLLADFAPVLGHKTYVQGK
                     LLSRLAQLGFIERRNGMIHEGPLLDVLLDYRTLADRIIHGTLGEVLAQAGHPPPVRDA
                     FTPDDDDLDDTGEDR"
     gene            20676..23477
                     /locus_tag="PKX01_08170"
     CDS             20676..23477
                     /locus_tag="PKX01_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ76139.1"
                     /translation="MFHIKQLELVHWDYWRRFTLPLDAQIITIVGPNGSGKTTLLDAL
                     RTLFGLRCSGKRDFRRYVRRAHRSFAWIRAVVANRPGSTGKRPFFPCLKDEVTLACRI
                     RRAGGDWTRDYAIADGDLPIEVLESQQDRAIEWVGYRDYQNRLAWGGLTPAIAKVLAL
                     EQGDTDKLCEYSPKALLELVFDVFGDKEVLDNYAAAREEQLNAEKELAALGLDLDRLR
                     AQAEAKRLEADRFLEWKQLDDEARALAAEIVPRLEAAELVRDIARERADLARGRLERQ
                     HGADELAALQQRLATVEAEKADAEAERRAVRQHDLDAEAAHLTAHDAVRDHEKLLAER
                     SALRTRLAREAGDAAVDLEAAYAKADAHLLHLQRQEREAIAAFEDKTQQLRATREHRG
                     PPAEPEVARFRARLREAGIAHRALAEIVEVTDPGWQSAVEAVLRPYRHVLLLAREQDR
                     HAAWSLGEAERFRHFIVPELEAAPPASAGSLAEVIRFADAVPRWLTDLLNRIQRVEDV
                     DDARRLPRDAEWITRDGYHRERRGARHLGKPADFHFGELARTARLKQLDTDLHSLDAR
                     LRDLRPQVEAAEEEAAGLRARLHGIEAAQLLAARVDDYDRAEDALPDARARLTAAIAA
                     RSEAKGALESLGLRLEGIGIEVDRRKREIGAVTRKLKDLADNAAPRRHEQAQRLLRLR
                     RLRHGMPADWRDPQQTALLAERYGDARGARAEHERLRTRLAEGDWITDDSVLTLAARL
                     KADLGQRERDYAEREAYCAATRRLTADARAAYIAKLRATVRQYGKNLRALGELANVDV
                     DCPVPLLENDDRALAAAGLEVRFDFDRKGAVGLNDGEASGGQQVMKSLILLIALLMDE
                     ARPGGFVFIDEPFAHLDIANIDRVGSFLRATRAQYLITTPVTHNANVFAPAQLTLVTR
                     KKKPGDDWAPPIGLIQRAV"
     gene            23486..>23854
                     /locus_tag="PKX01_08175"
     CDS             23486..>23854
                     /locus_tag="PKX01_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76140.1"
                     /translation="MDWSPPDADGLSSLRLDDPLRVSLKGQRRLRRGAPAPLDVDAWP
                     REERELLAAWVRQAGERPIRHDTLLRAAGPSRAAMADRLVHTLLRAGAVEVEEHGERG
                     LWRLRQLRFLAPAELRRALGL"
BASE COUNT         3954 a         8910 c         7390 g         3600 t
ORIGIN      
        1 cgatcaggaa gtagccgggg aagcccatct ggatgatggt gtcggtctcg aacttgaggc
       61 ggtcctcata gcgctgacgc tgggccgccc gctcgtccgg gtgcgggtag agctggacga
      121 ggcgttcctc caggcccttc ttcgcctcca ggatgaggaa gtcatccagg ctcatgccgt
      181 ccggcgtcgg gaagaggggc aggaagttct tgccgagctg cacggtgagg gagcagcgcc
      241 gggcgatctc cacgctgttg gccagcgcct cgggcagatc ggcgaacagg gcgcacatct
      301 cgtcctgggt cttgaagtac tgcgcttcgg tgaagtcccg cgcccgccgc ttgtccgcca
      361 gcaccgtgcc ctgggcgatg cacacccggg cttcgtgggc cttgaagtct tcgggacggg
      421 tgaactgcac cggatgggtc gccaccaccg gcagcgccag acgcgacgcg aggcccaccg
      481 cgtcgcggat gtaagcctcg gtgccaggat ggccggcacg ctgcagctcg atgtagaagg
      541 cgtccgggaa acgcgccgcc cagtctgccg ccaagcgctc ggcgagcgca agattgccgg
      601 cggccagggc ctgaccgatg tcaccgtccc tggcaccgga caggcacagc aggtcgctcg
      661 ccgtatccgc ggtgagccat tcgcggcgga tctcggcgcg cccccggtac ttgttctcca
      721 tgaaggcacg ggtcagcagc tcgcagagct ggccgtagcc ggcgcgattg cggcagatca
      781 gcagcacgcg ggcgggcttg tcccggtcgg cgtcgtgggt gatccagcaa tcggcgccca
      841 ccacgggctt gatgcccttg ccgcgggcgc ccttgtagaa cttgaccatc ccgaacaggt
      901 tggccagatc ggagataccc agcgccggca tgccgtcggc agcggcgcgg gcgatggcgg
      961 catccacctg cacgatcccg tcggagatcg aatattccgt atggagacgc agatggacga
     1021 aacggggggc gtgggattca gacatcccgc gattttaccc gccgccgcgg ctcgccggaa
     1081 acgccgtgac gaccggtccc cgcaacgccg ccgccgtgtt cagcgccgcg acgcctccag
     1141 cgaattgcgc aggcgcatct gcaggaggat gttgttggcc tgctgctcca ggtcgttggc
     1201 ctgctgtacc cacgccgcct ggcgcgtgga gatggcctgg gccagggctt tgagggcatc
     1261 gcgccgggcc gtggcgtagt ggatcagctc gacgcgctgc cagtcctgct cgggcgctgc
     1321 aaggtgatcc gacaggacac cgatctgacg gtcccaacgg ggaatcaggt cattgtccag
     1381 ggtatcgagt gcctgtacgg cagtgacccg acgatggcga agattgtcca tcagcaaggc
     1441 ctgttggcgc aggaggtcct gctcctccac cgagaactgc tgggcattct gctggaacag
     1501 ggtgttcatc tcgaaacgct gcacgacctg gggcgtgcgg atccacagca cgccgcccag
     1561 gaccagcgcc acgaccaggc caccgatggc aaggggcgac tgccagcgga cgggatcggg
     1621 ctccaccaac ggccgtgcca gggtggcgcc aagcagcagt ccgcccagca gaccgcccac
     1681 gtgcgccgca ttgtcgatgc ccggcaccgc gaacccggca aacagcgaat agccgatgaa
     1741 gcccagcgtg ccgctgcgca tctcggcaaa gatctgcacc ggcaccgacc cccgctgcgc
     1801 cgtcaagtag gccagcagcg cgccgtaaac cccgaagatt gccccggacg ccccgacgct
     1861 gactcccgac tgcttccacc acaggctggc gagattgccc aacagaccgg ctccgacata
     1921 gatgaccagg aaacccgaac gtccgtaaag gcgttcaacg acctgcccca cctggtgcag
     1981 cgcaagcatg ttgaggctca cgtgcagcaa gccgccatgc aggaacaggg cggtcgccag
     2041 gcgccaagct tcgcccttct ggaccagcgg cgcgaaattg cctcccaggc ggatcagcag
     2101 gtcggacgga aactgcatca gactgcccgc cgcaagggcc gtcagcagga agacgaggac
     2161 gttgagcacc accagcgccc gcgttccatc ggcccgcggc acgcgggaga acagcacatt
     2221 caggaaggtg tcgtgattca aggaaagccg ccgcaggaaa acctgaaaat tatacgcatg
     2281 cagttcgctt gagctcgacc gccagcggac gggcggcgga tcgtcggcga ggaacgaaag
     2341 tgtttcacac cacgaatggc aattgacgag ccgcgctaag atacgatccc gaacgtccac
     2401 cgcgcccatg atccacatcc tcaaacacgt cgaatcgatc acccagcagc gggaccgccc
     2461 gctccttgaa ctgggcgtcg cctctgccct cttcgaggtg gtgcgggcgc gcgaggtcaa
     2521 cctgttcaag gtgatctacg ggcagggcat gccgcgggtg gagcgggtca cccacgtggg
     2581 tcaggacggc gtccgctacg tggacacgga tttcgtcgat accatcgaga ccgaggtcat
     2641 cgatgcacgg gccgagatgg cagcctgtgt gatggaccgc cagatcgtac ggcgccacga
     2701 tctggatcgg ggcgtctatg ttcattgttt ccccgtggat gtggaccggc gcgtgctggg
     2761 cgtgctcgag ctggtcacgc cccatgtgct cgacgacgag cagatgggca ccgccgaggg
     2821 ctttctcggg ctgtaccgca actatctcaa catcctcgat tacagcgagc gggacaccct
     2881 gacgggcatg ctcaaccgca agaccttcga cgacaatctg gggaagctgc tcgccggcct
     2941 gacaccctcc ggcggaaacg gcagttccct gcccgcccgc cgtgccgtcg aatgtggccg
     3001 ccactggctg gcggtcatcg acgtggatca cttcaagcgc atcaacgacc attacggtca
     3061 tctttacggc gacgaggtcc tgttgctgct ggcgcgcatc atgcaacaga gcttccggca
     3121 ccaggacaag ctgttccgct tcggcggcga ggagttcgtg gtggttctgc gctcgtcggg
     3181 gcccggcgag acggccactg ccctcgagcg tttccggacc gcggtggaca cccacgtctt
     3241 tccgcagatc ggtcacgtca cggtgagcat cggctacacg ccgatccgcg caaacgacac
     3301 cattccggac atcgtcggac gggccgacga agccctgtat tttgccaagg accacggccg
     3361 caaccagacc gtcagccacg acgacctgct ggcgaagggt ctgatcgccc cccgcgagcg
     3421 cccccagggc gacgtggaac tgttctgaac cgaccggcgc cccgcggggc gcatccgacc
     3481 ggctcaggcg gagtgtcaat ccagccgttc agcggctggt cgcggcactc tccgcatcct
     3541 tccgcgcctg cttgcgatcc ccttccgccg catagatgag ccctttctgc tcgtcgctga
     3601 ggggggcgcc gagatcgcgc tcgagggcac tgtagatgcg gctcaggcgg tccgtgatcc
     3661 gccctctggc aggaatcgcc cgcatcacct gttcgcggct cgcccccgtg atgcccatca
     3721 ccttctgcac gaaggactcc tccaccttgc ggtgctccgc ctgcagccag gccgactgga
     3781 ccgggttgag caacgccgtc gaaggcggca gctgggcgcc ggccggcacc gacagggccc
     3841 acatcagggc ccacgccacg acactccgga cgggccatcg ggaaatcatc ttcatcatgg
     3901 aatcagtacc gtggatccgg tggtccgtcg tgcctccaga gcccggtgcg ccgcggcgac
     3961 atcctgcagc gcgtagcgct ggccgatgga caggcggacc tgaccggagg tgacgacggc
     4021 gaagagctcg tccgccatgg cgacgagctg ctcacggcgc gccgcgtaat ggaacaggct
     4081 cgggcgggtc aggaacaggg agcccatgcg ggccagcaca cccggatcaa aggcatccac
     4141 cggaccggac gcattgccaa aggacaccat catccccagg ggcgcgaggc aggccaggga
     4201 atccatgaag gtgtcgcgcc cgatggagtc atacaccacc tgcacgccct gcccccccgt
     4261 cagcgcgcgc acccgctccg acaccttctc gcgggtatag acgatggcgt gatcgcagcc
     4321 atgggcccgc gccagggccg ccttgtcatc ggagcccacc gtgccgatga ccgtcgcgcc
     4381 cagggctttc gcccactggc agacgatcag cccgacgccg cccgctgcgg catggatgag
     4441 gattgtgtcg cccgactgca cccggtaggt gcgccgcagc agatactggg cggtcagtcc
     4501 ctgcagcatc atcgcggcgc cctgctcgta gctgagcgca tcggggagga tcacgagacg
     4561 gtcggccggc agattgcgca cctcggcgta ggccccgagg ggaccgccgg cataggccac
     4621 ccggtcgccc ggtttcagcc cggtgacgcc cggccccacc gcctccacct cgccggcggc
     4681 ctccaggccg aggccggacg gcagcggcac gggatacagg cccgaacggt ggtagacgtc
     4741 gatgtaattg agtccgacgg catggtggcg cacccgcgcc tcgccttcac cgggcggcag
     4801 cggctcggac tcgcaccact caaggacttc cgggccgccg gtgcggctga agcggatctg
     4861 gtgactcatg gagcactccc cggggtcgga cggaaagccc gattgtaccg gatcgcaccc
     4921 ggccggcgcc cgcaccgccc gtcggcgggc gggcgccgac ggtggctcag accttttcca
     4981 cgcgctgttc ggctgcatcc accaccgcca gggcggtgat gttcaccaga cgacgcaccg
     5041 acgccgacgg ggtgaggatg tgcaccggca gggccgcgcc gagcaggatg ggacccacgg
     5101 acacgccatc cccgtttgcc accttgatga ggttgaaggc gatgttcgcc gcgtccaggt
     5161 tgggcatcac cagcaggttg gcttcgccgg tgagggtcga gtccggatgc aggcggtcac
     5221 gaatttcggc cgacagggcc gagtcgccgt gcatctcacc gtcgcattgc aggtcgggcg
     5281 cctgcacgcg caggagatca cgggctgcac gcatcttgcg ggacgacgcg gacggcgcgc
     5341 tgccgaagtt ggagtgggac aggagggccg ccttgggttc gatgccgaag cgcttgagct
     5401 cttcggccgc catcaccgtg atctcggcaa tctcctcggc accgggcatt tcattcacgt
     5461 aggtgtcggc aatggccagc acgcgcttgg gcagcagcag catgttcatg gctgcaaaca
     5521 ggctggcccc cttcttcagg ccgaccagat tcctgacgtg gttgaaatgg tggtcgtagg
     5581 caccggtggt accgcacacc agcgcgtcgg catccccgcg gcgcagcagc atgcagccga
     5641 tcagcgtggt gtcgctgcgc agggcttcgc gggcggaatc cggggtgacg ccgtcgcgcc
     5701 ccatgaggcg gtagtactcg tgccacagct cccggtagcg gctgtcggac tccacgttca
     5761 ccacgtcgaa atcacgcccg gcctgaaggt tgaggccgaa cttctggata cgcatgtcga
     5821 tgactgccgg ccggccgatg aggatgggcc gcgccagatt ctcgtccacc acggcgcgca
     5881 cggcgcgcag gacgcgctcg ctttcgcctt cggtgtacag cacacgcttc ttgtcggccg
     5941 ggatcccctt ggccttggag aacaccggct tcatgatgat gccggactgg tagacgaact
     6001 cggtcaggct ctggacatag gcgtccatgt cgatggcacg ggtcgccacg ccggattcga
     6061 tggcggcctg ggccaccgcc ggggcgatct tgacgatcag acgggggtcg aaaggcttgg
     6121 gaatcaggta gttcggcccg aagctcaggt ccgcgccgcc gtaggcctgg gccaccacgt
     6181 cggactgctc cgcctcggcc agctcggcga tggccttgac gcaggcgagc ttcatctcgt
     6241 cggtgatctt ggtggctgca acatccaggg caccgcggaa gatgaagggg aagcacagga
     6301 cgttattcac ctggttcggg tagtccgaac ggccggtggc gatgatggcg tccgggcgca
     6361 cctccttggc cagctcgggc atgatctcgg gcgtcgggtt ggccagcgcg aagatcagcg
     6421 gcttgtcggc catggtcttg accatctcgg gcttgagcac gcccgcggtg gacaggccga
     6481 ggaacacgtc ggcgccgacc atcgcatcgg ccagggtgcg gaaggccgtg ttctgggcgt
     6541 agcgggcctt ggtgggctcc atgttctcct cgcggcccac gtagatgacg cccttggagt
     6601 cgcacacgaa gacgttctcg cgacggatgc ccaggctgca ccacaggtcc agacaggcga
     6661 tggccgccgc gccggcgccg gaacacacca ccttgaccgc gccaatgtcc ttgcccacca
     6721 ccttcaggcc gttgagcatg gccgccgcag aaatgatggc cgtgccgtgc tggtcgtcgt
     6781 ggaagaccgg gatgttcatg cgctcgcgca gcttcttctc gatgtagaag cactcgggcg
     6841 ccttgatgtc ttccaggttg atgccgccga gggtcggctc cagcgcggcg atgatgtcga
     6901 tgagcttgtc cgggtcgttc tcggccagtt cgatgtcgaa cacgtcgatg ttggcaaact
     6961 tcttgaacag gcagcccttg ccttccatca ccggcttggc ggccagcggg ccgatgttgc
     7021 ccaggcccag caccgcagtg ccgttggtga ccacggccac caggttgccg cgggaggtgt
     7081 attcctgcgc atcggccgga tcggccacga tggcgtcgca ggccgcggcc acgccgggcg
     7141 aataggccag cgccagatca cgctggttgg tcaggccctt ggtgggcacc acggcaatct
     7201 tgccccgggt cgggctgcgg tggtactcga gggcggcggc gatgaaatcc ttgtccatgt
     7261 gttctctctc gtgtgaaaac ggctctgccg gctgccggaa ggcacccgca cgggaaagcg
     7321 gatgcggcgg cgtgccggtc ttgtcagccg gaaatctgaa atgtgtgcgg cggcgggtac
     7381 cccgactcgg ggccggtgct ctcgttgaca gtgccacggg catgcccgcg acaccggagt
     7441 ttcgccaccg ggaggaggtc gcgaagacta catgttgttt ggccggagtg tacccgagcg
     7501 ggcgcatttg cgcaccctcc ggcacggccg gggtgattgc atggcacaat cgcgcgcaac
     7561 gacaggcatg tcaatcaaca atgatccggg gaggcacgat gcggcgcgtg gtattcaacc
     7621 agaagggcgg agtgggcaaa tccaccatca cctgcaacct ggcggcgatc agcgcccacc
     7681 agggcctgcg cacgctggtg gtggacctgg atccccaggg caattcgacc cattacctgc
     7741 tggggcacgc cctcgaaaac gccgacgaca ccctcgcggg gctcttcgag cagaccctcg
     7801 ctttcaagtt caatccccgc cagacagccg aattcgttca gcccacgccc tttcccggtc
     7861 tctcgctgct gccctcccac cccagcctcg aagagctgca gagcaagctg gaatcccgct
     7921 acaagatcta caagctgcgc gatgccctgg acgaactggc cgaccggttc gacgccgtct
     7981 atatcgacac cccgccggcg ctcaacttct acacccgctc ggccctcatc gccacgcgcc
     8041 gctgcctgat tcccttcgac tgcgacgact tctcccgccg ggcgctctac gcgctgctgg
     8101 agaacgtgca ggagatcaag gccgaccaca accgcgatct ggaagtggag ggcatcgtgg
     8161 tgaaccagta ccagccccgc gccagcctgc cccagaaact ggtgcaggaa ctgattgccg
     8221 aaggtctgcc catcttcaag acctacctgt ccagctccat caagatccgc gagagccatg
     8281 aacaggccaa gcccatgatc cacctggagc cgcgccacaa gctggcccag gaattcatcg
     8341 ccctgcacgg cgagctgaag ggctgagcgc cggcccgtcg ggcgtggcga ccggggccgg
     8401 cggcggcacc cggctcggcg tcgctcaggc ggccgtcagt tttgccatcg acagcatcag
     8461 ccatttgctg ccggcagcgc caaacttgac ctgcacccgc gcatcggccc cgctgccctc
     8521 ggccgccacg atcaccccgt cgccgaagcg cgcatggcgg acattctggc cgatcgcaaa
     8581 tccgcccaga tcccgcgact tgagcggtgg cgagaccggc atcacggccg ccgccggacg
     8641 cgccgacgac ggcgtgaagt aacgcgccgg agattccgcc gcaaagccgc cgtgacgggt
     8701 attgggcggc gtcagccatt tggtcagacc gtccggaatc tcttccagaa agcgcgaacg
     8761 caggttgtag cgcacctggc cgtggagcat gcggctctgg gtgaagctca agtacagacg
     8821 ctggcgggcg cgggtcacgg ccacgtacat caggcgccgc tcctcctcca gcccttcgct
     8881 ttccaggatg ctgttctcgt ggggaaacag cccctcctcc agcccggaca ggaagaccac
     8941 atcgaactcc agacccttgg ccgcgtggac ggtcatcagc tgcaccgcct cgctgcccgc
     9001 gtcggcctgg tggtcgccgg cctccagcga ggcgtgggca aggaattcgg cgagcacggt
     9061 ggccgtgtcg ggcgcacctt cggaggcgcc gttcacctgg aattcggcgc tgaagttggc
     9121 cgccgcgttg atcagttcgt ccaggttctc gaggcgctcc tggccttcct tctcaccctt
     9181 gtaatggtcc cgcagcgtgg agcgttccag cacgtgatcc accctctcgt gcagcggcag
     9241 atcccgcgtc tcgtggcgca ggcgttcgat cagggtgacg aactgggcca gcgccgtgcc
     9301 cggcttgccg gacaggtgcg ggacggtctg gtagaggggc gtgttccagg tccgcgccgc
     9361 gtcctgcagg gcctccagcg agcgggcgcc gatgccgcgg gtcgggaagt tgaccacccg
     9421 caggaaggcc gtgtcgtcat ccggattggc cagcaggcgc aggtaggcca gggcgtgctt
     9481 gatctcctgg cgctcgaaga agcgcaggcc gccgtacacc cggtaggcga cgccgttgga
     9541 gaaaagctga tgctccagca cccgcgactg ggcgttggaa cggtacagca gggcgacgct
     9601 ggagcggctc acgccatccc gcaccaggga ttggatctcc tcgacgatcc agcgcgcctc
     9661 gtcggtatcg gtgaaggcct cgtagacgcg gatcggctcg ccggcgcccc ggtcggtcca
     9721 tagatgcttg cccagacgct cccggttgtg ggcgatcagc gcattcgcgg catccaggat
     9781 gttgccgtgg gagcggtagt tctgttccag gcggatcacg tcggtcacgt gaaaggcccg
     9841 ctcgaaatcc cgcatgttgc cgatctcggc cccgcggaag cggtagatgg actggtcatc
     9901 gtcccccacg cagaagaccc gcgcgccgcc ctcccgtccg ccgtcggcga gcaggctcaa
     9961 ccagcggtac tgaaggcggt tggtgtcctg gaactcatcc accaggatgt gacggaagcg
    10021 cgcctggtaa tgcctgcgca ggggctcgtt gcgctccagc acctcgtagc agcgcagcaa
    10081 caactcaggg aaatccacaa ctgactcgcg ctggcactga gcctcatact ccgcgtaaag
    10141 ctccaccttg cggcgtgtga agtcgtccca ggcctccacg gctggcggtc ggatgcccgc
    10201 ctccttctgg ccgttgatga agtgctgcag gtcgcggggc ggaaacttct cgtcgtcaac
    10261 attgagcgtc ttgagcaggc gcttgatggc ggccagctgg tcgcccgagt cgaggatctg
    10321 gaacagctgg ggcaggcccg catcccggtg atgcgcgcgc aggaagcggt tgcacaggcc
    10381 gtggaaggtg ccgatccaca tgccgcgggt gttgatgggc agcagcgcag agaggcgcgt
    10441 cagcatctcc ttcgcggcct tgttggtgaa ggtcacggcg aggatgccct gagggctgca
    10501 gtggccgttc tggatcagcc aggcaatgcg ggtggtcagc acccgggtct tgcccgaccc
    10561 ggcgccggcc aggatcaggg catggcgcgg tggcaaggtg acggcagcga gctgctccgc
    10621 gttgaggtgt tcaaggagct gggacatggt gacgtgaata cgggggaggc gcgcattcta
    10681 cgccccgatt catcgatcca gcccgaacgg gcagtgttgt aaaaatgcga gttccgtcac
    10741 gacgggggtg aaacaaaatg caaggcagct tgcaccgtct ggcggacttg cctaaactga
    10801 aattgtgcaa tgcaacagaa agggtgttca gagatcggaa gccacaaaca acgcaatgat
    10861 ttttttgata aaaatgaata tttgcgttgc aacaaaacca gaccagaaat gtgtgcaacc
    10921 ggaacccttt tcgcatccca acaggagtta acagcatgaa atctcccaag atcctcccct
    10981 ggtacgcccg caaggccggc gtctccgtcg aacgggccga agccttgtgg cgcaaggccg
    11041 tccgcgaagc caccgccgac accggctggg tcggcacctc cgaattctgg ggcgctgccc
    11101 tggatcgctt cctcgaactg ctgcaggacg agcagaccac cctctgtcag ccgcacatgg
    11161 aaacctttgt gcgcagccag caccgcatgt ggctgctgcc cctgctggcg gccgaagaca
    11221 tgttcaacgc gatgtctgcc aactggcagc gctatcgcaa ccagatgcgc aaggcggcct
    11281 gaaacggccg caaccggcgc gcctgacgcg ctgcgccgga gcctcacggt gtttttctcc
    11341 ctcctctccc tcgtggagat tcaacggcgt cctccccgga cgccgtcttt ttgtccacgt
    11401 cccgcccttc aagccacccg aggcgctcac ccgctgctgc tataatcggc gactttgaga
    11461 cgttccaccg gagtttaatg tgctgatttc caacgctttt gcccaagccg cccccgctgc
    11521 cgcgcaggat cccaccgccg gctacggcca gatcgtgatc atggtggcga tgttcgcgat
    11581 cttctggttc atgctgatcc gcccgcaaca gaagcgcgcc aaggaacaca aagcgctgat
    11641 cgaaggcctg caaaagggcg atgaggtcgt cacccagggc ggtctggtgg gcaaggtcgc
    11701 ccaggtgggc gacaactacg tgcatctcga gatcgccgcc aacacccagg tcgtcgtcca
    11761 gaagcccgcc atcggcatcg tcctgcccaa gggcaccctc aagtccctct gatgcctgcc
    11821 gccggggcac gccccggccg gtaccgctct ccgccccgtt tcacgccttt ccgcccgacc
    11881 atgaatcgct atcccctctg gaagaacctc ctcatcggcc tcgtgctgct gtggggtctg
    11941 ctctacaccc tgccgaactt ctacggagag gtgccggccg tccaggtctc gagcggcaag
    12001 gccaccctca aactggatgc caccgccacc ctcaagcgcg tcgaggacac cctccatggc
    12061 gcagggctcc agcacgacgg gctgttcgcc gatctcacca gcgtccgcgc ccgcttccac
    12121 gacaccgaca cccagctcaa ggccaaggac gccatcgaga aggccttcaa cccggacccc
    12181 gccgacccgt cctacgtggt cgccctcaac cttctgtccg ccagtcccca gtggctcacc
    12241 gccatccatg ccctgcccat gtatctgggc ctcgatctgc gcggcggcgt gcacttcctg
    12301 cttcaggtgg acatgaaggg cgccgtcacc aagcgtctcg acgccaccgc cggcgacctg
    12361 cgcaccctgc tgcgggacaa gtccgtgcgc catgccggca tcagccgcga aggtcagagt
    12421 gtggcgatcc gcttccgcga cgccgagacg ctcgcccgcg gtcgcgcggt gatcaccgcc
    12481 agcaccaatg acctgctcct cagcgaaggc caggacggcg ccgatttcaa gctgacggcc
    12541 accctcaacc cgctggccca gaagaacatc caggaatcgg cgatccgcca gaacatcgtc
    12601 accctgcaca accgcatcaa cgaactgggg gtcgccgaac cggtgatcca gcagcagggc
    12661 ggcgaccgca tcgtggtgca gctgcccggt gtacaggacg tggccaaggc caaggacatc
    12721 ctcggccgca ccgcgaccct cgagatccgc atggtggacg acgccccggg cgccctcgaa
    12781 gcggccctca acggcagcat cccgccgggt agcgaactgt actccgagcg cggcggcagc
    12841 cccctgctgg tcaagaagca ggtggtgctc accggcgaac gtctcaccga tgcccagccc
    12901 ggcttcgacg gccagaccca cgagcccgcc gtccacctga ccctcgacgc ggccggcgcc
    12961 cgcatcttca aggacgtcac ccgcgagaac gtcaacaagc gcatggccat cctgctcatc
    13021 gaaaagggca agggcgaagt catcacggcg ccggtcatcc gcggcgagat cggcggcggc
    13081 cgggtgcaga tctccgggcg catgagctcc gaagaagcca ccgacaccgc tctgctgctg
    13141 cgcgccggct ccctcgccgc gccgatggag atcgtcgagg agcgcaccat cggccccagc
    13201 ctgggcgccg acaacatccg caagggcttc cactccaccc tgtggggctt cgtcgccatc
    13261 gccgccttca tgtgcctgta ttacatgctg ttcggcctgg tgtcggtgct ggccctgggg
    13321 tccaacctgc tcctgctggt ggccctgctg tccctgctgc aggccaccct gaccctgccg
    13381 ggcatcgccg ccatcgccct caccctgggc atggccatcg acgccaacgt gctcatcaac
    13441 gaacgtatcc gcgaagagct gcgcaacggc tccagccccc aggcggccat ctccgccggc
    13501 tacgagcgcg ccttcgacac cattctcgac tccaacgtca ccaccctgat cgccggcatc
    13561 gccctgctcg tcttcggcag cggcccggta cgcggctttg cggtggtgca ctgcctgggc
    13621 atcctgacct ccatgttcag cgccatcctg gtctcccgcg gcctggtcaa cctgatctac
    13681 ggcaagaagc gcaagctcga gaaactggcc atcggccagg tctggaaacc cggcctgtaa
    13741 gccccgcgcg ccccatcgaa tcggactccc atcatggaat tcttccgcat ccacaaagac
    13801 atcccgttca tgaagcatgc gctggtgttc aacgtgattt cgttgatcac cttcctgctg
    13861 gcggtgttct tcctggtcac caagggcctg cacttctccg tcgaattcac cggcggcacc
    13921 ctcatggagg tggcctacac cgagccaccg accctcgagc gcctgcgcga cgccctcgcc
    13981 cacgaaggct atgccgacgc ccaggtgcag aactttggca gcgcccgcga cgtgttgatc
    14041 cgcctgccca accgggaagg catggagacc gccaaggtca gcgaaaaggt catggccacc
    14101 ctcgccgccg tggaaggccc caaggccgag ctgcgccggg tggagttcgt cggcccgcaa
    14161 gtgggcaagg aactcgcctc cgacggcgcc atggccctgc tgctggtgat cggcggcatc
    14221 gtcctctacc tggccctgcg cttcgagtgg cgcttcgccg tgtcggccat cgtcgccaac
    14281 ctccacgacg tggtcatcat cctgggcttc ttcgccttgt tccagtggga attctccctg
    14341 ccggtgctgg ccgccaccct cgccgtgctg ggctattcgg tgaacgaatc ggtggtggtc
    14401 ttcgaccggg tccgcgaaac cttcaaaaag aagcgtggcc tcaccacacc cgaggtgctc
    14461 gaccacgcca tcaccagcac catcagccgg accatcatca cccacggctg cacccagatc
    14521 atggtgctgt ccatgctcct gttcggtggc gagaccctct actacttcgc cctcgccctg
    14581 accatcggca tctgcttcgg catctactcc tcggtgctgg tcgccagccc gctggtgatg
    14641 tggctgggcg tctcccgcga acagttcatc caggtcaaga aggacaagca ggaagcggtg
    14701 gtctgagcgc gccgcgcgcc agcctgccgg gccgcccctg tgcggcccgt ttcatttgag
    14761 ccgccgatgc ccgatcccgc gccccgcctg cacgactgcc tggccgacgc cagcggtgga
    14821 ctgcgcttcc acctgcgggc ctggcgccat cggcgccgct gggccgcatt ccacgccgcg
    14881 gtggccgact ggttgcgcgc ctggcatcct gaccggcccc acctcgtcct cgtcggcccc
    14941 agcgccggct acgatctgcc ggccggctgg ctgggcggat tcacacgcat cacggtgctg
    15001 gagcccgacc ctctcgcccg ctggctgctg cgccgtcgcc ccgacgcaac ccggctgcat
    15061 ttcgacgacc tggactgcct cgccaccccc gacggtctgg cccacctgaa agcccgcttc
    15121 ccggacgccg ccatcctctt ctgcaatgtc ctcggccagg tcaccgcacc cggcggccgg
    15181 ggctgggccg aactgtgcgc ctgccacctg cgcgacctgc cctgggccag cctccacgat
    15241 gtggtctcca cccctcgtgc gccccgtgcc gacgcggtcc gcgcatcgtt tccgggcgga
    15301 acctccctcg aggccgtgct cgccgccttc tggccggggg gcgagctgcc cctcgtggat
    15361 cacgccacct tcgccctggg cggcgatgtc cctcaccact acgccctgtg gccactgcgg
    15421 cccggtcgct ggcacctggt ggaatgggtc gcccacgcca tgcaaaatga cacgtcggcc
    15481 atgccggacg acgcggccgg ccgtggacgg gccgtgtaga atcgtccggc cgatttcgcc
    15541 cagcgatcca ccatgcacgt ccttcgactc gcctgccgcc tgctcctcgt caccctgctg
    15601 ctcgccgcgg gcggcgcacg ggcccaggcg ctcaacgaca cctatgaagg cctgctcctg
    15661 cccgacacct tcgagcagcc catcccggtc accgtcaagc tcaccgagat gggcaccctc
    15721 ctcctcggcc aggtcaagac cctcgccccg ctgcccggcg aggccgccct gtcccgcgcc
    15781 gaacgcaaca acgcccgctg cctgatcttc gcccccatcg ccgcagccac ccacctgcgc
    15841 atggaaggca cctgcgttcc cggcctcttc gaaggcaact tcctgatctc ccgcaacggg
    15901 cgtgacaccc gcggcacctt caccctgcct cgcaaggccc cggccgcccc gagcgccgac
    15961 cccgccgatg cgccgcgccg cgcggccccc gtggacgccg cgtccccgat caccgcctgc
    16021 ctcaaggcca acaccgcctg cctcatcgcc tgtccgcggg gcgattacaa cgtggaattc
    16081 ctctgctcca accgctgccg ccagaaattc cagcactgca agggcaagag caccctccag
    16141 acgcccgccc aacccgccac cggcagcacc accccgtcgg ccaactgacc ccgttcgggc
    16201 gcccccgaag gtataatgca gggttttccc gcattcccag ggtccccgaa catggaagcc
    16261 gaacgcctca acgccctctc caacgccctc gccgacctca aggcccgcga gctcgaactg
    16321 cggaggtatc tttgactacg ataccaaggc cgaaaagctg atcgaagtca gcaagcagct
    16381 cgaagacccc gaggtctgga acgacgcgcc ccgcgcccag gaactcggca aggaaaagaa
    16441 gaccctcgaa ggcgtcgtcc tcaccctgga tgaaatcgac cagggcatcg ccgacggtcg
    16501 cgaactcttc gagctcgcct ccatggaaga ggacgaagag acggtccagg ccgtcgaagc
    16561 cgacgtcgcc cagctccagg gcaagatcgg cgagctggaa ttccgccgga tgttcagcaa
    16621 tccgatggac gccaacccct gcttcatcga aatccaggcc ggcgccggcg gcaccgaagc
    16681 ccaggactgg gccggcatgc tcgaacgcat gtacctgcgc tactgcgaac gcaagggctt
    16741 ccaggtcgag ctgatggaag agtccgaagg cgaagtggcc ggcatcaaga gctgcaccat
    16801 caaggtgggc ggcgaatacg cctacggctt cctgcgcacc gaaacgggca tccaccgcct
    16861 cgtgcgcaag agccccttcg actccaacgc ccgccgccac accagcttca cctcggtgtt
    16921 cgtcttcccc gaggtggacg actccatcga gatcgacatc aacccggccg acgtgcgcac
    16981 cgacacctac cgcgcctccg gcgccggcgg ccagcacatc aacaagaccg actccgcggt
    17041 gcgtctcacc cacatcccta ccggcatcgt cgtgcagtgc cagaacgacc gttcccagca
    17101 ccgcaacaag gacgaagcct ggtcgatgct gcgcgcgcgc ctctacgaag ccgagctgcg
    17161 caagcgccag agcgagcagc agaagctcga ggatgccaag agcgacatcg gctggggcca
    17221 ccagatccgc tcctacgtgc tggaccagag ccgcatcaag gatctgcgca ccagctacga
    17281 agtgggcaac acccaggccg tgctcgacgg cgacctggac ggcttcatcc aggccagcct
    17341 caagcagggc gtctgagtcc ccccgcgcat ggtccgcctc ctcgcctggc tgtccggcgg
    17401 cctcgcgctg ctcctcgtcg cgctcgccgg cctgctcgcg ctggcggtgg aggaccatcc
    17461 cctcgtcgtc gccgatgacg ccctgtcccc tgccgcgatc agccgcgcgc gggaactcct
    17521 gcgccgccat gacccgcgca agctggtccg gggcgagacc cgcaaggtgg atctcggcgc
    17581 cgacgatctg gcgagcctcc tgcgcttcgc cacccgccgc ggcctgcatg ggcagagcgc
    17641 cctccgcctc gaacagggac acgcggaggt ccgcttcacc gcgcccctgc ccgccacgcc
    17701 tctggggcgc tacctcaacc tgaccgccga cctgggcgtg agcgacggcc gcatcgacat
    17761 cgacggcgtg cgcatcggcc gcctccccgt ccccgcaccg ctgttctctg cgctgttcga
    17821 cgcctacgtc cgccacaccg atctggcgcc ggagctcgcc ctcctgcgcc gcaccgtcac
    17881 ccgcgtggcc atcgccccca acgctctcag cttcgtctac gtgtggcaac cggagctcct
    17941 tgacagcgcc cgcgacctgg ccatcggccc gacccgccgt cagcgcctcg ccgacgccca
    18001 cctgcgcctc gtcgcccgca tggatgccgt cgccgcccgc ggtccccgcg cgcccctggc
    18061 cgacgtgctc ggcccgctcc tcgccggcgc cggcgaggcc cccgctggtg gcgagcgcag
    18121 tgctgcctac cgggacgccc tgctggtggc cgccttccac ctgtccggca aggacgtggg
    18181 cgccgtgatt cccgaagcgc gccagtggcc gcgaccgcgt ccgctgaccc tcaccctgcg
    18241 tggccgcgag gatctcgccc agcacttcac agtctccgcc gccgtcgccg cggcgggtgg
    18301 cgagcccctc gccaacgcgg tcggcctcga caaggaaatc gacgactccg accacggcag
    18361 cggcttctcc ttcgtggatc tcgccgccga ccgggccggc acccgcctgg gcgagctggc
    18421 cgtacgcaat cccgaacgtc tcgccgccgc tctggccgcc ggcctcaccg acagcgcctt
    18481 gctgcccccc atcgacggcc tgcccgaagc catgatggcc gccgaattcc gccagcgctt
    18541 cggcggcccc gacgcccctg cctaccgacg catgcgcgac gagatcgaac ggcgcatcgc
    18601 cgccctcgcc ctgttccgct gacgcgtcgt ccccatgagc gaagtccgcc tcgaagagct
    18661 cctcgcgccg ggtgtcgtcg ccgacgacgt gccggagctc gacaccctgc tgcgcgcacg
    18721 tcccctgctc gccgccttca gcaccctctt ccacggcagc gaggccgaag tgctggcccg
    18781 cctgctggtg ctgcgcgaga tcggtcgcga cgcccagacc acccactgga cgcccgagcg
    18841 cctgcgccag cgcttcacct acctcgaccc ggtcaagctc gaaaccgtcc tccgccgcct
    18901 gcgcggcaac ggcctcctcg acatcggcga cgacaaccgt tacaccctct ccgacctggg
    18961 ccgcaatgcc gtcgccgcgg tggacatgct ggtcggcttc agcgccggcg acgacctgga
    19021 actgggcttc ctgaccgccc agctcgccgg cctgcaggcc gtcggcagcg tcactccgga
    19081 agccctcggc cacctgctcg gcaagctcaa cgacctcgcc tggcacttcg aggacgccat
    19141 cgcctccggc tccgaatttc gcatcgccga cgcccgccgg cgcctgtccg ccaacacccg
    19201 ctggatcgaa aagggcaccg aagtcctgcg caccctcctc gccgaccccg agctgccctt
    19261 cgacctggcc cgcctggccc agcccatcgg cctcgcccag tcgcgcctgg cgcgcatcga
    19321 cgccgccttc cagcgcgccg tcaacaagat cgaggcccag cgcgtcaccc ttggcggctc
    19381 cggcatctcc tccagcgacg tggccgcctg gctgcgcggc cgcgacgccc gtgccctggc
    19441 cgccctcgcc cgcaacgccc tggcggccgc accggacctc accctgctcg ccggcggcca
    19501 cgagctcctc gaccgggccg agctggccct ctccgaagac gcccgccgca tggaagccga
    19561 caccgccctg ccgccgcccg aagaagcccc gcgccgggac gccccggaac aggaagacct
    19621 gcgcctgctg gagcacttca gccgccgcct ggcccgcctc gccgagcccg gccaggcgcc
    19681 cgagaccctc gcccgcgccg tcgccggcgg cggattcccg gccgcctcct accgcctgtc
    19741 cctcctcgcc ctcctcgccg atggcggcga cagcgacacc gccagccccg ccgacggccc
    19801 catcggcgac ttcatgcgcc tgccggtcga catccgcttc gagctcgcca ccgagcccgt
    19861 caacgaagac gagatcgcca ccatgagcgc cggcctggtc ggcgcccgcg gccgtctgcc
    19921 cgccgccgaa gccgtccccg tcctcgaccc cgacaccctg cccagacctg cctgatggaa
    19981 caccagatcc gcaccctcac cgcccgtctg ctggcccacc gcagcctgcc ccgcagcgac
    20041 ccgctggtgc gcctcgccct ggtggacgac gccttccgca gcgacctgga cgcccgcctg
    20101 ggcgccgtcg gcctcagcct catcgacaac ccctacgccg cccacgtggc cgtcgccctc
    20161 gccgaggaca tgcacgaacc cgtcttcggc ggcggccgcg aatacgccgc cagcaacgcc
    20221 ggcctcaccc gcgaccagct cgccctgctg gtggtcatct ggtccctcat cgtcctgccc
    20281 aagcgggagc gccaggttaa tcgccagcgc ctcaccgacg acggtcagga agacatgttc
    20341 ggcaaggaag gccccctggc ccacggcggc gaagtctccg gcgccctctc cgaagcctcc
    20401 ctgctggccg acttcgcccc ggtcctcggc cacaagacct atgtgcaggg caagctcctg
    20461 tcccgcctgg cgcagctcgg cttcatcgag cggcgcaacg gcatgatcca cgaaggcccg
    20521 ctcctcgacg tgctcctcga ctaccgcacc ctcgccgacc gcatcatcca cggcaccctc
    20581 ggcgaagtcc tcgcccaggc cggccacccg ccccccgtgc gggacgcctt cacgcccgac
    20641 gacgacgacc tggacgacac cggggaggac cgctgatgtt ccacatcaag cagctcgaac
    20701 tggtccactg ggactactgg cggcgcttca ccctgcccct cgacgcccag atcatcacca
    20761 tcgtcggccc caacggctcc ggcaagacca ccctgctcga cgccctgcgc accctcttcg
    20821 gcctgcgctg ctccggcaag cgcgacttcc gccgctacgt gcggcgcgcc caccgcagct
    20881 tcgcgtggat ccgcgcggtc gtcgccaacc gccccggcag caccggcaag cgccccttct
    20941 ttccctgcct caaggacgaa gtcaccctcg cctgccgcat ccgccgcgcc ggcggagact
    21001 ggacccgcga ctacgccatc gccgacggcg atctacccat cgaagtcctc gaatcccagc
    21061 aggacagggc catcgaatgg gtcggctacc gggactacca gaaccgcctc gcctggggcg
    21121 gcctcacccc ggccatcgcc aaggtcctcg ctctggagca gggcgacacc gacaagctct
    21181 gcgaatactc gcccaaggcc ctgctggagc tggtcttcga cgtcttcggc gacaaggagg
    21241 tgctggacaa ctacgccgcc gcccgcgaag agcagctcaa cgcggagaag gaactcgccg
    21301 ccctcggcct cgacctggac cgcctgcgcg cccaggccga agccaaacgc ctcgaagccg
    21361 accgcttcct cgaatggaag cagctcgacg acgaagcccg cgccctcgcc gccgagatcg
    21421 tgccccgcct ggaagccgcc gagctggtcc gtgacatcgc ccgcgagcgc gccgacctgg
    21481 cccgtggccg cctcgaacgc cagcacggcg ccgacgaact ggccgcgctg cagcagcgcc
    21541 tcgccaccgt cgaagccgaa aaggccgacg ccgaagccga acgccgcgcc gtgcgccagc
    21601 acgatctgga cgccgaagcc gcccacctga cggcccacga cgccgtgcgc gaccacgaaa
    21661 agctcctcgc cgaacgcagc gccctgcgca cccggctcgc ccgcgaggcc ggcgacgccg
    21721 ccgtggacct ggaagccgcc tacgccaagg ccgacgccca cctgctccat ctgcaacgcc
    21781 aggaacgcga ggccatcgcc gccttcgagg acaagaccca gcagctgcgc gccacccgcg
    21841 agcaccgcgg cccacccgcc gaacccgaag tcgcccgctt ccgcgcgcgc ctgcgggaag
    21901 ccggcatcgc ccaccgggcc ctcgccgaga tcgtcgaagt caccgatccc ggctggcagt
    21961 ccgccgtcga ggccgtcctg cgcccctatc ggcacgtcct gctgctggcg cgcgagcagg
    22021 accgtcacgc cgcctggtcc ctgggcgaag ccgagcgctt ccgccacttc atcgtgcccg
    22081 agctggaagc cgccccgccc gccagcgccg gcagcctcgc cgaagtcatc cgctttgccg
    22141 acgccgtgcc gcgctggctc accgatctgc tcaaccgcat ccagcgcgtc gaagacgtgg
    22201 acgacgcccg ccgcctgccc cgtgacgccg aatggatcac ccgcgacggc taccaccggg
    22261 aacgccgcgg cgcccgccac ctgggcaaac ccgccgattt ccacttcggc gaactggccc
    22321 gcaccgcccg cctcaagcag ctcgacaccg acctgcacag cctcgacgcc cgcctgcgcg
    22381 acctgcgtcc ccaggtcgag gccgccgagg aagaagccgc cggcctgcgc gcccgtctgc
    22441 acggcatcga ggccgcccag ctcctcgccg cccgcgtcga cgattacgac cgcgccgagg
    22501 acgccctgcc cgacgcccgc gcccgcctca ccgccgccat cgccgcccgc agcgaggcca
    22561 agggcgcgct ggaaagcctc ggcctgcgcc tcgaaggcat cggcatcgag gtggaccggc
    22621 gcaaacggga gatcggcgcc gtcacccgca agctcaagga cctcgccgac aacgccgccc
    22681 cgcgccgtca cgaacaggcc cagcgcctcc tgcgcctgcg ccgcctgcgc cacggcatgc
    22741 ctgccgactg gcgcgacccg cagcagaccg ccctgctcgc cgaacgctac ggcgacgccc
    22801 gcggcgcccg cgccgagcac gaacgcctgc gcacccgcct cgccgagggc gactggatca
    22861 ccgacgacag cgtgctcacc ctcgccgccc gcctcaaagc cgacctgggc cagcgcgagc
    22921 gggactacgc cgagcgcgaa gcctactgcg ccgccacccg tcgcctcacc gccgacgccc
    22981 gcgccgccta catcgccaaa ctgcgcgcca ccgtgcgcca gtacggcaag aatctgcgcg
    23041 ccctcggcga gctcgccaac gtggacgtgg actgcccggt gcccctgctg gagaacgacg
    23101 accgcgccct cgccgccgcc ggcctcgaag tgcgcttcga cttcgatcgc aagggcgccg
    23161 tcggcctcaa cgacggcgaa gcctccggcg gccagcaggt catgaagtcc ctgatcctgc
    23221 tcatcgccct gctcatggac gaggcccgtc ccggcggctt tgtcttcatc gacgagccct
    23281 tcgcccacct ggacatcgcc aacatcgacc gggtcggcag cttcctgcgc gccacccgcg
    23341 cccagtacct catcaccacc ccggtcaccc acaacgccaa cgtcttcgcc ccggcccagc
    23401 tcaccctggt cacccgcaag aagaaacccg gcgacgactg ggcgccaccc atcggcctga
    23461 tccagcgggc ggtctgagca cagccatgga ctggagcccg cccgacgccg acggactctc
    23521 cagcctgcgc ctggacgacc ccctgcgggt cagcctcaag ggccagcgcc gcctgcgtcg
    23581 cggcgccccg gcgcccctcg acgtggatgc ctggccccgc gaagaacgcg aactcctcgc
    23641 cgcctgggtg cgccaggcgg gcgaacgccc gatccgtcac gacaccctgc tgcgcgccgc
    23701 cggcccttcc cgcgcggcca tggccgaccg cctcgtacac accctgctgc gggccggcgc
    23761 cgtcgaagtg gaggagcacg gcgagcgcgg cctgtggcgc ctccgccagc tccgcttcct
    23821 cgcccccgcc gaactgcgcc gcgccctcgg cctg
//
LOCUS       DAOQPA010000045        23836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1005_length_23836_cov_18.818595, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000045 DAOQPA010000000
VERSION     DAOQPA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23836
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_23836_cov_18.818595"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKX01_08180"
     CDS             complement(<1..583)
                     /locus_tag="PKX01_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HMZ76141.1"
                     /translation="MAEQPIQRGRLRPAPEHFLNEADVQAVEAGRRGFLRKALVTASA
                     AMAATTVARAEDGDPAILTLPPWTTSLGQPVAARPYGLPSQYEKGLQRRESPGLTRMS
                     QVSVAFTPLQGLFGIVTPSGLHFERHHQGWSDIDPSKHRLMINASDDGFIRAPRVYTV
                     DDILRLPPVSRMHFIECGANTGMEWGNVAVPTVQ"
     gene            829..1143
                     /locus_tag="PKX01_08185"
     CDS             829..1143
                     /locus_tag="PKX01_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ76142.1"
                     /translation="MKLLQWSAVLCLAGTAGAALAADRPDPELARNLAATCANCHGTD
                     GRAVPGAGNEALAGVDKAKLLQKLQDFRSGAKPATIMHQIAKGYTDAQLDLIAAYFAA
                     RK"
     gene            1156..2433
                     /locus_tag="PKX01_08190"
     CDS             1156..2433
                     /locus_tag="PKX01_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ76143.1"
                     /translation="MTNRRDFLRSAALAGSAAAFPGLSGCALGSRPDGHVVVVGGGYG
                     GATLAKYLRMWSERSVSVTLIERNPQFISCPMSNLVIGGEKTLADITLSYDGLKHTWG
                     VQVVQDEVTAIDPTARTVRLASGGSLRYDRLVLSPGIEFMFDQVPGLESDAARSTVLH
                     AWKAGPQTVALRQQLADMRDGGVYALSIPKAPYRCPPGPYERACLVASYLQRNKPRSK
                     VLILDANEEIVSKKALFTKAFEKYKGILEYLPNSELREVDAASRTAVLEFDKVKADVL
                     NVVPPQRAGDVAAKSGVRLINNRWVEVDWLSFEAVGTPGVHVIGDALFPAPTMPKSGH
                     MANQHAKVAAAAILNLLAGQPPNPTPVVMNTCYSFVDPKNVIHVSSVHQYDAAKKVVL
                     PVPGAGGVSAAANELEGKYAHSWARNIWADMLT"
     gene            complement(2510..3112)
                     /locus_tag="PKX01_08195"
     CDS             complement(2510..3112)
                     /locus_tag="PKX01_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ76144.1"
                     /translation="MGLRVRGAVLAACWVVATSAGAEDVKRAEEIVGGRCFLCHGMEG
                     EAASALYPRLASQHAEYIAKQLADFKSGRRKSDTMAGMVADLTPDEMKSLGLYFQAKP
                     ARHGEAGDADLAGVGRYIFTKGNPYSGVAACTACHGGKGHGTPQLPRLAGQQALYLEN
                     QLKQFNTRERTNDNAVMHSIASKLTELELKAVSAYISTLD"
     gene            complement(3206..4834)
                     /locus_tag="PKX01_08200"
     CDS             complement(3206..4834)
                     /locus_tag="PKX01_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76145.1"
                     /translation="MSVRFHSLKARLALLGLISVLGVCLLSALSLIQTTRIKANLVVF
                     ADRHIALNHHATRTYAQGLQMGQALRNIVLDPANRKAYENFVQASESFKEVFGALEPL
                     VKAEDAAAAEELAQRVAAWRPLQAQVLALVQGGEADAARALLTTRETPAWRAVREILL
                     AQVKRSDAAAEGARVSLLADVDTIAGGAAVVGVATLAVVLGATLWVGRAVFRQVGGEP
                     SATAASLARIAAGDLTGEIRPRAGDRDSILAAASDMQAHLAGLVRSIGRGAVSMMQAS
                     EAIARNAESVADTAERQSQATSGIAAAVEELTVSIGVMSDNAVHAASLSGQTEGKVQD
                     GLTQVSATTDTIRQVAEGMTAFAETMGELSSKVSGINGIVQAIHEIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAERTATSTQEISAMIAGVQDSTRVALDNMARTRAV
                     ALEGARHMDQTQEAVADLSTVAAGVREEVDAIRAALDEQRAASTDIARRVEQIAQGIE
                     GTHVSAAASSRSAQELVALAHELEAMTGRFRVSA"
     gene            complement(5106..5750)
                     /locus_tag="PKX01_08205"
     CDS             complement(5106..5750)
                     /locus_tag="PKX01_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ76146.1"
                     /translation="MYFRQFFDPATSAMSYLLADPAARAAAILDPLGDPCQALVFRAL
                     LAEHALDLHLILLTHVHGNALEDIRSTYPDVRTVAGVGPVTGVSVAREGDRLHLGREE
                     IRIIATPGHTFHSVSYLWRDRLFCGDALELGGCAASVEGEGDAGQLFDSVTGRLFALA
                     DETLVFPGHDFNGRTVSTIGEERRRNPFFQSLNRDAFTAALRTRDAADRQFRWP"
     gene            complement(5761..6060)
                     /locus_tag="PKX01_08210"
     CDS             complement(5761..6060)
                     /locus_tag="PKX01_08210"
                     /inference="COORDINATES: protein motif:HMM:NF021140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon YbgE family protein"
                     /protein_id="HMZ76147.1"
                     /translation="MSRRDADTGPHVPRDPMARALALLTGAFAAFLLLCFPQVLVRSD
                     GVADPWAAVCLIGAMITGLASGVGTPFERRWTRALLSPESCLLALTLATLRVAAH"
     gene            complement(6075..6686)
                     /gene="petA"
                     /locus_tag="PKX01_08215"
     CDS             complement(6075..6686)
                     /gene="petA"
                     /locus_tag="PKX01_08215"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HMZ76148.1"
                     /translation="MTCETWAPGACDPARRSLLRATAGAGSVLAVATAVPFVASLTPS
                     QRARAAGAPVEVDIDKLAPGELMVVEWRGKPVWILRRTPEMLASLARAEQHLADPGSA
                     VDAQQPDYARNAHRSIRPEIFVAVGLCTHLGCSPSSVFEPGHGALGADWPGGFLCPCH
                     GSTFDLAGRVYRNKPAPTNLEVPPHTFLSDTRLRIGADAGTPA"
     gene            7036..8370
                     /locus_tag="PKX01_08220"
     CDS             7036..8370
                     /locus_tag="PKX01_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMZ76149.1"
                     /translation="MSHPAHPLPELVSFLESLPEPHILCDRSYRILAANAAYRAKCAR
                     PEDLVGRTCYEVSHRYPVPCDRAGESCPLALSLQSGQRERVVHLHHTSSGERYEKIEL
                     SPVRDARGEIAYFIEKLEPLPVARGVAGGEGLIGRAATFRGMLELVARVAPSEATVLL
                     QGESGTGKELVARAIHDASRRADRPFVAVDCSGLTETLFESELFGHERGAFTGATVRK
                     VGLVEAASGGTLFLDEMGDIPLSMQVKLLRLLETGTYRRVGSTELVRADIRLISATHR
                     PLKSLVGDGRFRQDLFYRINTFPIALPALRERREDIPLLAEALLRRVAPDRRLGVAPD
                     ALDDLLAYDYPGNVRELRNILERASLLCDGDTLERRHLPDDLLPATALSASTASYAVT
                     TGGLPDDATLATLLRSFRGTRRALATHLGVSERTLYRRIREMDVADPPPGGA"
     gene            complement(8422..8799)
                     /locus_tag="PKX01_08225"
     CDS             complement(8422..8799)
                     /locus_tag="PKX01_08225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ76150.1"
                     /translation="MYSVQVDRSPVITVNGAKHAVSYAVDGSQMNPLEAFYATLAGCA
                     AVFAKKACKELGISPEGIHIACRPFAGAAGPLSLSKFKTEVRFPEGIDAAQKAAVLEA
                     IKHCAVKQIVEAGAAVDFQVAEG"
     gene            complement(8820..9272)
                     /locus_tag="PKX01_08230"
     CDS             complement(8820..9272)
                     /locus_tag="PKX01_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76151.1"
                     /translation="MIVFTAFLAGLVFGIGLIVSGMANPSKVTGFLDLAGAWDPSLAF
                     VMAGAIALGVVGYRVALRRGRPVFGAALQIPTLRNIDRRLVVGSALFGIGWGIAGICP
                     GPGLVMLGAGLPEGLVFVSAMLVGMALFELQQGHLARLLAALGIARNS"
     gene            complement(9283..9717)
                     /locus_tag="PKX01_08235"
     CDS             complement(9283..9717)
                     /locus_tag="PKX01_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HMZ76152.1"
                     /translation="MNIDWTHFTPQAALTGGALIGLATGLLLVLNGRIAGISGILGGL
                     LQRCSVSELSWRAAFVAGLVVAPALFGLFLPLPEIRVDARWGGLVVAGLLVGYGARLG
                     SGCTSGHGVCGLSRMSPRSLVATLTFMGTGFMTVFVVRHLLA"
     gene            complement(9723..10049)
                     /locus_tag="PKX01_08240"
     CDS             complement(9723..10049)
                     /locus_tag="PKX01_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ76153.1"
                     /translation="MSDTETLPDLEAIRQAASAATAVLSALANENRLLLLCQLSRGER
                     SVGELEAELDLHQPTLSQQLSVLRAENLVATRRDGKRVFYSIADVRVLALLDTLYEQF
                     CPRKDV"
     gene            complement(10307..10383)
                     /locus_tag="PKX01_08245"
     tRNA            complement(10307..10383)
                     /locus_tag="PKX01_08245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10347..10349),aa:Met,seq:cat)
     gene            10611..11279
                     /locus_tag="PKX01_08250"
     CDS             10611..11279
                     /locus_tag="PKX01_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HMZ76154.1"
                     /translation="MPYILRSVLVLTALSGLALPVQALSPALAPNASVTVDRAGVFDY
                     YVLSLSWSPTFCLTHADNSQCSKGYGFVLHGLWPQYARGGFPQDCYSPERLTPEAVRF
                     GRTIFPAENLIDHEWRKHGTCSGLSALAYFRAADRARTQINVPAPLESPATTHSDTAA
                     AIARSFVAANPGIPSGSVTVVCSGPQLSEVRLCLNTSLAPVACGKGVTHACRGGPIRI
                     PSVR"
     gene            complement(11626..12078)
                     /locus_tag="PKX01_08255"
     CDS             complement(11626..12078)
                     /locus_tag="PKX01_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HMZ76155.1"
                     /translation="MCKSARSVLFRLFLWCALVVSVPAWANDHGGGGGGSDLVNMETI
                     TTNLAPEPGGNVRFIQLTIALRLHDPSGAAAISAYMPQIRNDILGLLASRTGAELLIT
                     KAREDLSDDIQDAVNAVVGTAAYTDKKGHAHRAEGPVKRALFTTFIVQ"
     gene            complement(12394..12466)
                     /locus_tag="PKX01_08260"
     tRNA            complement(12394..12466)
                     /locus_tag="PKX01_08260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12431..12433),aa:Thr,seq:tgt)
     gene            12610..14169
                     /locus_tag="PKX01_08265"
     CDS             12610..14169
                     /locus_tag="PKX01_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ76156.1"
                     /translation="MPDAPQTDALTEGGDAPGQPIRLLFIEDSENDMLLLLHHFRSAG
                     YAPHFRRVASEAGLLDALADTTWDVILCDHKLPGFSAQGALRLIQERALDLPFIIVSG
                     VIQEDEAIAAMRAGAHDYLSKSKLDRLIPAVERELREVCNRREKRLAEQTLRESEARQ
                     RALTDNIPGVVFRMGFTSDGMLGFQYLSEAASMLFGTSADELIGGSAGWMTWLLDDDL
                     PGFIEAADLSAAHLSTLNWEGRIQLSSGEQKWINLRSSPRLSEAGEVVWEGVMWNITH
                     SKRVESDLRESRSQLAALSNHLQRIKEDERERIARDVHDVLGGTLVAMKFEMSLLESK
                     LESHPAQARERARNVGRMVDDAIATVGRVTRELRPGILKDFGLPAAIESQGEDFAQRT
                     GIQVKILCNDHDLAPDYDTALALFRVFQEALTNVSKHARASQVGVRLLQEGDEVLLEI
                     SDDGVGIDAADLQKPRSFGLRGIRERMSSLGGRLDVDPVLPHGTRLTLRAPLYDPTAT
                     ERPPQLRSHAR"
     gene            14166..14807
                     /locus_tag="PKX01_08270"
     CDS             14166..14807
                     /locus_tag="PKX01_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76157.1"
                     /translation="MTKTIRVLIADDHAIVRQGLRQILSDTPDLTVAGEAENGVQAVQ
                     MVRNGEWDVVLMDVSMPDRNGIDALKLIKKEFPRLPVLILSMYPEEQYAIRALKSGAA
                     GYLTKQSAPELLVTAIRQVASGKKYVSPSLAEELVNAIGEDTERPAHEKLSDREYQTL
                     CMIASGKTPTEIAEALNLSVKTVSVYRARLLEKMHLRNNAELTHYGLKHGLAE"
     gene            14936..16738
                     /locus_tag="PKX01_08275"
     CDS             14936..16738
                     /locus_tag="PKX01_08275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ76158.1"
                     /translation="MMENRPPAPRRSKMIDTSQPSDLARETLRQLALRKIAPTPDNYR
                     ALYHEIAGTQAEDGFPDRMLRQIGAALPRHSRDAVRFAQAFEDATARQDWQAVKTSLT
                     EAFTALDVPELNWPALLRELITEWDRRQAGLTQARKREMLDRVLSASGAHPERLHERL
                     QALVRAWARNSDDDSTDSTAATESGEVRAQAERMTSDTPVPADSSGVELLQRLLTGLE
                     AVVVHLDPEAAHELQAIAAHLQPGAPSAPVGELRTRLDRLLSHLNWVAEDHAALRDAL
                     LRVLKLIVDNISKLVMDDQWLRGQMSLLDEVFSHPLDIRTLGELESRLRDVIARQAAL
                     KHELSDAHAKLKDMLARFVDRLGEFSDSTGDYHDKIEQCAGRISTARDISELTDVIAE
                     VMKETRSIQESTLRSRTELEELRGRVENANVEINRLQNELEQTSELVRHDPLTGTLNR
                     KGLDDAFARETALARRRNTRLCVALLDVDNFKHINDTHGHQTGDEALQHLAAVIRENL
                     RPQDSVARYGGEEFVILLPDTDVDLATATLVRLQRALTKRYFLARQQKLLITFSAGVA
                     ELQADEDTESALDRADKAMYIAKRSGKNRVISAT"
     gene            16800..17177
                     /locus_tag="PKX01_08280"
     CDS             16800..17177
                     /locus_tag="PKX01_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76159.1"
                     /translation="MRPFACCPSPAPSVLTGPAVLVAVLLLTATPADAEPSGRLFHTP
                     EERQRLDRLLDNAASGRAQPERLDGIITRGDGRTTLILDGHPRPAAADLRPLDARSAR
                     ILANDGQTHRLRVGERLPPLSVP"
     gene            17213..18376
                     /locus_tag="PKX01_08285"
     CDS             17213..18376
                     /locus_tag="PKX01_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76160.1"
                     /translation="MVALALVVLLALGATGWFLARRDSNAARHLAREQRTADALRTAR
                     DALIGFAATYRNSLHPDADFGYLPCPDLDGDGSSETCGVQGDTRVGRLPTLTLGLPDL
                     RDGDGECLWYAVAGSFKNNPKADILNPDSTGRFQLLDAAGAPRSLPGDQAGLAAAVIF
                     APGPPLPTQQRQSSTGRCGGDLSASAVGAYLESLQPLSGPGVLTVDPRGSTGNDRIVW
                     ITGDDIMRALKARASFATHLNTVLDATADCLRRAGVPAPTRIESTANGLQLGRVPAFS
                     GNAGACTSAGLRDAVENWGELMRYARCTDGTACLSSATGPCRGVLLFGGERLPAGQPR
                     VTDADKALAAHYLESTTLAALSARQLGGLPSRILIPFARRSEPASADVAVCLP"
     gene            18409..19122
                     /locus_tag="PKX01_08290"
     CDS             18409..19122
                     /locus_tag="PKX01_08290"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ76161.1"
                     /translation="MTDRPSRSSARGFTLVEMAVVLLILAILAGGLTAGLSAQVARRN
                     EAETDAALDEARDALLGYALRRGTLPCPARSGSDGAEDRESLTTCRGNRLNGLLPWAE
                     LGISGLDAWGRRLHYAVAPDYVKAITLDSAGKLDIRTRNSAGTELTLTTAIGAAPALV
                     LSHGPNGLGGIDADGNALAAPPAGSDEARNATAGSTLFYARSLSENSDAAGGAFDDRV
                     VWVSPYLIANRLASAGRLP"
     gene            19267..20574
                     /locus_tag="PKX01_08295"
     CDS             19267..20574
                     /locus_tag="PKX01_08295"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ76162.1"
                     /translation="MSVSAVHRLLARLAGVPRPSSDQEDAWLERTFFVLTGLLTAGAS
                     ALSELFRQVAGAGTSPGITVFLVLGIGLNLAASRRRADFRPCAHVQASLILGAPFLAH
                     LLQTDLIGDAGRLPWAMLAPLGTLLCLDARASRRWFVTLLGVTAGTVAFDQSRLPRHP
                     GMPDGVTLLAVAVDLTLLAGMLYFLLHFAVTQKVQTRQRLRAAHRRLRDEQQHAESLL
                     LNILPAPIAARLKLSRGTLADGHPEVIVMFADIVDYTRIASGMQPTEVFEMLNRVFCR
                     FDELAEHHGLERIKTIGDAYMVAGGLHAHPNEPHAAMAAMALDMQAAVRDHAVTGGIT
                     LELRIGVASGPVVAGVVGRRKFIYDLWGDTVNVAHRLCTEAQPSQIQCDSRLYERVRG
                     RFVFAAPVLLFLKGKGYVPVYRLRSPRVLAVHREPPHPSHRAA"
     gene            20713..21384
                     /locus_tag="PKX01_08300"
     CDS             20713..21384
                     /locus_tag="PKX01_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ76163.1"
                     /translation="MAHSQLTSRQRGFTLVEIAVVLVIIGLLLGGVLKGQELINSAKV
                     KSLINDFRNVATFAYAYQDRFRALPGDDAGATSHLSGGVKASTGGTAGNGRIEGNWNS
                     TTATDESVLFWQHVRLANLASGDGRNPGLEGLVILDWLPRNAAGGRTGITGVAPITGW
                     AGNFFVCQDNIPGSLARQVDIAMDDGQPAAGSVRLLANGSASGSAVALADLADGTPYT
                     VCAAL"
     gene            complement(21385..>22379)
                     /locus_tag="PKX01_08305"
     CDS             complement(21385..>22379)
                     /locus_tag="PKX01_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMZ76164.1"
                     /translation="LWLCAGGEFGFVLLSEIAGAELLPTSLMQVVVAALVLSMLLAPL
                     IVQASEKIVLRLVPSEWMSRSLQLTSIATQSMFVDKHAILCGFGRNGQYLGRLLEQEG
                     ITYIALDLDPERVREAAAAGETVVFGDAAKRETLVAAGIARASVVVVTYADTDAALRA
                     LSQVRSVRPDVPVVVRTADEVDLEKLQEAGAAEVVPESVESSIMLASHALALLGVPLP
                     RILKRIREIRAERYHLLRGIYRGAHLHEEAVEERQQARLHSLTLEAGAYAIGRTLAEL
                     HLRELGVEVSALRRKRIRVVDPTPETRLELDDVLVLLGTPAQLANTETRLLKGL"
     assembly_gap    22380..22479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22480..23513)
                     /locus_tag="PKX01_08310"
     CDS             complement(<22480..23513)
                     /locus_tag="PKX01_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ76165.1"
                     /translation="MVNTLELVLILLAGAVFVVGLFRSMNLPPVLGYLMVGAAVGPHA
                     MKLMPDTGGARYLAEFGVVFLMFSIGLEFSLPRLFAMKRIVFGLGAFQVLATMAFVTV
                     VGWLAGLGWGASFALGGTLAMSSTAILSKLLSDRLELDSKHGRETIGVLLFQDLAVVP
                     LLILIPALSGPAEALMLTLGLATAKAVGMLALVLFFGQKLMRGWFFHVARQRSPELFM
                     LNVLFITLGLAWMTESAGLSMALGAFLAGMLISETEFRYHVEEDIKPFRDVLLGLFFV
                     TVGMFLDVGEIVAHLAAVLAMLALLLVGKFVIVAAASRIMGSAQGTAVRTGLWLCAGG
                     EFGFVLLSEIA"
     gene            23593..>23836
                     /locus_tag="PKX01_08315"
     CDS             23593..>23836
                     /locus_tag="PKX01_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HMZ76166.1"
                     /translation="MTPTPHPAARPQAVELARRVLRIEADAVAALAERLDAVAFTRAL
                     DLILASRGRVIVSGVGKSGHIGRKLAATLASTGTPAY"
BASE COUNT         4063 a         8880 c         7299 g         3494 t
ORIGIN      
        1 actgcaccgt gggcaccgcc acgttgcccc actccatgcc ggtgttggca ccgcattcga
       61 tgaagtgcat gcgcgacacc ggcggcaggc ggaggatgtc gtccacggta tagacgcgcg
      121 gcgcccggat gaagccgtcg tcactggcgt tgatcatcag gcggtgcttc gacgggtcga
      181 tgtcgctcca gccctggtga tggcgctcga aatgcaggcc cgacggggta acgatgccga
      241 acaggccctg cagcggcgtg aaggccaccg acacctggga catccgcgtg agccccggcg
      301 attcccgccg ttgcagacct ttttcgtact gggacggcag gccgtagggc cgcgccgcca
      361 ccggctggcc caggctggtg gtccacggcg gcagcgtgag gatggccgga tcgccgtcct
      421 ccgcacgggc caccgtggtg gccgccatgg ccgccgacgc ggtgaccaag gccttgcgca
      481 ggaagccgcg gcgtcccgcc tcgaccgcct gcacgtcggc ctcgttcagg aaatgttcgg
      541 gcgcaggacg caggcgtccc cgctggatgg gctgctcagc catctgggtg tctccccctt
      601 cggttgtcat cgggctcggg gcgggcatgt cttggcgcat gcgtaaaatc tgcgagcgcc
      661 ccttgatacg aatcaatcta taaccataca ctaatatcgt caattactaa tattcgtgca
      721 gtgcacgaag attcagagcc ggaaccaccg gccagcgccc cgatccagag agaccaggag
      781 gacgccacac catctccgcc gaccggcgga acacaggagg agagacccat gaaactgctt
      841 caatggagcg ccgtgctgtg cctggcaggc accgccggag cggcgctggc ggcggatcgc
      901 cccgatccgg agctggcccg caatctggcc gccacctgcg ccaactgcca cggcaccgac
      961 ggccgggcgg tgcccggcgc cggcaacgag gcgctggccg gcgtggacaa ggccaagctg
     1021 ttgcagaagc tccaggattt ccgttccggc gccaagcccg cgacgatcat gcaccagatc
     1081 gccaagggat acaccgacgc gcagctcgac ctgatcgcgg cgtacttcgc cgcccgcaag
     1141 taagggggac ggaccatgac gaatcgacgc gattttctgc gcagcgcggc cctcgccggc
     1201 agcgcggcgg cctttcccgg gctctccggc tgcgctctgg gcagccgccc cgacggccat
     1261 gtagtggtgg tgggcggcgg ctacggcggc gccaccctgg ccaagtacct gcgcatgtgg
     1321 agcgagcgga gcgtgtccgt gaccctcatc gagcgcaacc cccagttcat ctcctgcccc
     1381 atgtccaacc tggtgatcgg cggcgagaaa accctggccg acatcacgct gagctacgac
     1441 ggcctgaagc acacctgggg cgttcaggtg gtgcaggacg aagtcaccgc catcgacccc
     1501 accgcccgca cggtgcgtct ggcctccggc ggcagcctgc gttacgaccg gctggtgctc
     1561 tcgccgggca tcgagttcat gttcgatcag gtgccgggcc tggagagcga cgccgcccgc
     1621 agcaccgtcc tgcacgcctg gaaggccggc ccccagactg tcgccctgcg ccagcagctc
     1681 gcggacatgc gtgacggcgg cgtgtacgcc ctgtcgatcc ccaaggcgcc ctaccgctgc
     1741 ccgcccggcc cctacgagcg cgcctgcctg gtggccagct atctgcagcg gaacaagccc
     1801 cgctccaagg tgctgatcct tgacgccaat gaggagatcg tctcgaagaa ggccctgttc
     1861 acaaaggcct tcgagaaata caagggcatc cttgagtacc tgcccaacag cgagctgcgg
     1921 gaggtggatg ccgccagccg cacggcggtg ctggagttcg acaaggtcaa ggccgacgtg
     1981 ctcaacgtgg tgccgcccca gcgcgccggc gacgtggcag cgaagtccgg cgtcaggctc
     2041 atcaacaacc gctgggtgga ggtggactgg ctgagcttcg aagcggtcgg cacccccggc
     2101 gtacacgtga tcggtgacgc cctcttcccg gcgccgacca tgcccaagtc cggccacatg
     2161 gccaaccagc acgccaaggt cgcggccgcg gcgatcctca acctcttggc cggccagccg
     2221 cccaacccga cgccggtggt gatgaacacc tgctacagct tcgtcgatcc gaagaacgtc
     2281 atccatgtga gctccgtgca ccagtacgac gccgcgaaga aggtcgtgct gcccgtgccc
     2341 ggtgccggcg gcgtctcggc ggccgccaac gaactggaag gcaagtacgc ccattcctgg
     2401 gcccgcaaca tctgggcgga catgctgacc tgacgcccgc cccggccggc ccgcccccag
     2461 cggacacggg ccggccgcag gcagacggcc gccaggcttt cggcgcccct cagtccaggg
     2521 tcgagatgta ggccgacacg gccttcagtt ccagctcggt cagcttcgag gcgatggaat
     2581 gcatcacggc gttgtcgttg gtgcgttcgc gcgtgttgaa ctgcttgagc tggttttcca
     2641 gatacagggc ctgctggccc gccagccggg gcagttgcgg cgtcccgtgc cccttgcccc
     2701 cgtggcaggc ggtgcaggcc gccacgccgg agtatgggtt gcccttggtg aagatgtagc
     2761 gccccacgcc agccagatcg gcatcgccgg cttctccatg acgggccggc ttggcctgaa
     2821 agtagaggcc gaggctcttc atctcgtccg gagtcaggtc cgccaccatg ccggccatgg
     2881 tgtcgctctt gcgccgcccg ctcttgaagt ccgccagctg cttggcgatg tattccgcat
     2941 gctggctggc caggcgcgga tacagggcag acgccgcctc gccttccatg ccatgacaca
     3001 ggaagcagcg cccgccgacg atctcttcag cccgctttac gtcttcagcc ccggcgctgg
     3061 tggccacgac ccaacaggcc gccagcacgg cgcccctcac acgaagtccc atgtccatct
     3121 cccccttttg gttttgtgca gaattataga atataagaat ttattgatta tctatcaatc
     3181 ctcaaggcct ctcacgacgc ccggctcagg cgctcacccg gaaccgcccg gtcattgcct
     3241 ccagttcgtg ggccagggcg acgagttcct gcgcacttcg actggatgct gcggccgaca
     3301 cgtgggtgcc ctcgatcccc tgggcgatct gctcgacacg gcgcgcgatg tcggtgctgg
     3361 ccgcgcgctg ctcgtccagg gcagcgcgga tcgcatcgac ctcctcacgc acacccgcgg
     3421 ccacggtcga caggtcggcc accgcctcct gggtctggtc catgtggcgc gcgccctcca
     3481 gggccaccgc tcgggtgcgc gccatgttgt cgagggcgac gcgggtactg tcctgcacgc
     3541 ccgcgatcat cgcggagatc tcctgggtcg aagtggcggt gcgctcggcg agtttgcgca
     3601 cctcgtccgc cacgaccgca aatccgcgcc cctgctcgcc tgcccgggcc gcctcgatgg
     3661 cggcgttgag ggccagcagg ttggtctgct cggcgatctc gtggatcgcc tggacgatgc
     3721 cgttgatgcc gctcaccttg gacgacagct cgcccatcgt ttcggcaaag gccgtcatac
     3781 cctcagccac ctgacggatg gtgtccgtcg tggccgacac ctgggtcagt ccatcctgca
     3841 ccttgccctc cgtctggccc gagagggagg ccgcgtggac cgcgttgtcg gacatgacgc
     3901 cgatgctgac ggtcagttcc tccaccgccg ccgcaatgcc cgacgtggcc tgactctgcc
     3961 gttcggcggt gtcggccacg ctctcggcat tgcgcgcgat cgcctcactg gcctgcatca
     4021 tggagaccgc gccgcggccg atgctgcgca ccagcccggc cagatgcgcc tgcatgtcgc
     4081 tcgcggccgc cagaatgctg tctcgatctc ccgcccgcgg acggatctcc ccggtcagat
     4141 ctccggccgc aatacgtgcc agggacgccg ccgtggcaga aggctcgccg ccgacctgac
     4201 ggaacaccgc gcgtccgacc cacagggttg cgcccagcac gacggccagg gtcgcgacac
     4261 ccaccaccgc cgccccgccg gcgatcgtgt ccacgtcggc gagcaaggac acccgcgcgc
     4321 cctccgcagc ggcgtcactg cgcttcacct gcgccagcag gatctcccgg acggcgcgcc
     4381 aggccggcgt ctcgcgagtc gtgagcagcg ccctggcggc gtccgcctcg ccgccctgca
     4441 cgagggccag cacctgcgcc tggagggggc gccaggctgc gacgcgctgc gcgagttctt
     4501 ccgcagcggc ggcatcctcc gccttgacga ggggctccag ggcgccaaag acctccttga
     4561 agctctcgga cgcctgcacg aaattttcgt aggccttgcg gtttgccgga tccaggacga
     4621 tgttgcgcag ggcctgcccc atctgcagcc cctgggcata ggtgcgggtc gcatggtgat
     4681 tgagggcgat gtggcgatcg gcgaacacca ccagattcgc cttgatccgc gtcgtctgga
     4741 tcaatgacag cgccgacagc aggcagaccc ccagcacgct gatgaggccg agcagcgcca
     4801 gacgcgcctt caaggaatga aagcgtacag acatgatgtt tctccccggt caaaccttgc
     4861 agccatccac gctgtgtcgc gcggtacctg ttgttacggt caggcggccg aggacttgac
     4921 catttcgaca tcatggtcat gtcgccacgc gcctttcaga cccggacgca tccggggcgt
     4981 ttcgccgaca gccgcgccca tgccgattca gggcgccgcg cggcgcccgc aaccgcgaca
     5041 tccgcacgcg ccatcgcgca tcacccttcg acgcctttca ttcgatgcgc cgcgccgcgc
     5101 cacgatcagg gccaccggaa ctgccgatcg gccgcatccc gcgtgcgcag ggccgcggtg
     5161 aaggcgtctc ggttgaggga ctggaaaaag gggttgcggc ggcgctcttc gccaattgtg
     5221 gacaccgtgc ggccgttgaa atcgtgtcct ggaaacacca gcgtctcgtc cgccagggcg
     5281 aacagtcgcc cggtcaccga atcgaaaagc tggccggcat cgccttcacc ctcgacggac
     5341 gcggcgcaac cgccgagctc gagggcgtct ccgcagaaca gacggtcacg ccacagatag
     5401 ctcacgctgt ggaacgtatg gccgggcgtg gcgatgatgc ggatctcctc acgcccgagg
     5461 tgcagcctgt cgccctcccg cgcaaccgac acgccggtca ccggcccgac cccagccact
     5521 gtccggacgt ccggataggt cgaccggatg tcctccaggg cgttgccgtg aacatgggtc
     5581 agcaggatca ggtgcaggtc cagcgcgtgc tccgccagca gtgcgcgaaa gaccagcgcc
     5641 tgacacggat caccgagggg atcgagaatc gccgccgcgc gggccgccgg atcggccagc
     5701 agatagctca tggccgacgt ggcgggatcg aagaattgcc ggaagtacat gattgcaccg
     5761 tcaatgcgcg gccaccctca gggtggccag ggtcagggcc agcaggcagg actcggggga
     5821 cagcagggcg cgcgtccagc gccgctcgaa gggcgttccg acaccactcg ccagcccggt
     5881 gatcattgcg ccgatgaggc acacggcagc ccatggatcc gccacaccgt cggaccggac
     5941 caggacctgc ggaaaacaca gcagcaggaa ggccgcaaag gcacccgtca ggagggccag
     6001 cgcccgcgcc attggatccc tgggcacgtg cggcccggtg tctgcatcac gtcgcgacat
     6061 gtcgggctcc gtgctcaggc gggcgtgccg gcatcggcgc cgatccgcag gcgggtatcc
     6121 gacaggaagg tgtggggcgg cacctccaga ttggtcggcg ccggcttgtt gcgatacacg
     6181 cgtccggcca ggtcgaaggt ggaaccatgg caggggcaca ggaagccgcc gggccagtcg
     6241 gcgcccagtg cgccatgccc gggctcgaac acgctggacg gcgaacagcc cagatgggta
     6301 cagagaccca cggcgacaaa gatctcgggc cggatggaac gatgggcgtt gcgcgcatag
     6361 tctggctgct gggcgtccac ggccgacccg gggtcggcca gatgctgctc cgcgcgggcc
     6421 agggaggcga gcatctcggg cgtccggcgc aggatccaca ctggtttgcc acgccattcg
     6481 acgaccatca gctcgccggg cgccagcttg tcgatatcca cctccaccgg cgcaccggcc
     6541 gcgcgggccc gctgggaagg cgtcaggctg gccacgaagg gaacggcggt ggccacagcc
     6601 agtacgctgc cagctccggc agtggcccgc agcaggctgc gccgggcggg atcgcaggcg
     6661 ccgggcgccc aggtctcaca ggtcatcggg tctctccgtc agggggctca cgggacacgg
     6721 gagccgttct tgattggtga tggagacgac ctatcaagct ccgtgccaag cattcggcct
     6781 gtaaaacatc attgaaatcg ggcacttgcc gtgcacgagg cgcttctgca cagccataac
     6841 tgccacgccc gcgctgccac attccgccaa tcggcagatc tggcagcgca ccccaagcag
     6901 cgcgcgccgt gccgtacagt gcacggacgc ccccgtcgac gcctccccgg tccgatcgcc
     6961 cccgctcgga cggattgacg cccgcccgcg gccctgacag catgtgtcag tccccttggc
     7021 agcccgcctc cgcccatgtc ccatcccgcc catcccctgc cggaactggt gtccttcctg
     7081 gaatccctgc cggagcccca catcctgtgt gaccgcagtt accgcatcct ggcggccaac
     7141 gcggcctacc gggccaagtg cgcgcggccc gaggacctgg tcggacgcac ctgctacgag
     7201 gtgtcccacc gctaccccgt gccctgcgac cgggccggcg aatcctgccc gctggccctc
     7261 agccttcagt ccggccagcg tgagcgggtc gtgcatctgc accatacgtc cagcggcgag
     7321 cgctacgaga agatcgagct ttcaccggtg cgtgacgccc gtggcgagat cgcctacttc
     7381 atcgaaaaac tggagccgct tccggtggcg cggggcgtgg ccggcggcga aggcctgatc
     7441 gggcgcgctg cgaccttccg gggcatgctt gaactggtgg cgcgggtcgc tccatcggaa
     7501 gccaccgtgc tcctgcaagg cgaatcgggc accggcaagg aactggtcgc ccgcgccatt
     7561 cacgacgcca gccggcgcgc cgaccggccc ttcgtggcgg tggattgttc ggggctcacc
     7621 gagaccctgt tcgaaagcga actgttcggc cacgagcggg gcgctttcac aggcgccacg
     7681 gtacgcaagg tcgggctggt ggaggccgcc agcggcggca ccctgttcct cgacgaaatg
     7741 ggcgacatcc cgctgagcat gcaggtcaag ctgttgcgtc tgctggaaac gggcacctac
     7801 cggcgcgtcg gcagcaccga actggttcgc gccgacatcc gcctgatctc ggcgacgcac
     7861 cggccgctga aaagcctggt cggcgacggc cgcttccgcc aagacctgtt ctaccggatc
     7921 aacaccttcc ccatcgccct gccggccctg cgggagcgcc gcgaggacat cccgctgctg
     7981 gccgaggccc tgctgcgccg ggttgcgccg gatcgccgac tgggcgtcgc acccgacgcc
     8041 ctcgatgatc tgctggccta cgactacccc ggcaacgtgc gcgagctgcg caacatcctg
     8101 gagcgggcca gtctgctgtg tgatggagac accctggagc gccgccacct gccggacgat
     8161 ctgctgccgg ccacggccct gagcgcatcc accgcctcat acgctgtcac cactggcggg
     8221 ctgccggatg acgcaacgct ggccaccctg ttgcgcagct tccgcgggac ccgccgggcc
     8281 cttgccaccc acctgggcgt cagcgagcgg accctctacc ggcggatccg cgagatggac
     8341 gtcgcagacc cgccgcccgg cggggcctga aggcgccccg cgtcgttcgc gttcccgggc
     8401 caggcggcgg cggaccgatc ctcagccttc ggcgacctgg aaatcgacgg ctgcgccggc
     8461 ctcaacgatc tgcttcacgg cgcaatgctt gatcgcctcg aggaccgccg ctttttgcgc
     8521 ggcgtctata ccctccggaa aacgcacctc ggtcttgaac ttcgacaggg acaaggggcc
     8581 agccgcaccg gcgaagggcc tgcacgcgat gtggatgcct tccggcgaaa ttcccagttc
     8641 cttgcaggcc ttcttcgcga acaccgcagc gcagcctgcc agcgtggcgt agaacgcttc
     8701 gaggggattc atctgcgagc cgtcgaccgc gtagctgacc gcgtgcttgg cgccgttgac
     8761 ggtgatgacg ggggaacgat ccacttgaac ggagtacatc tggatttcct ttgcgtaatt
     8821 caggagttgc gcgcgatgcc gagggctgcc agcaggcgcg caagatgacc ctgctggagc
     8881 tcgaagagcg ccatgccgac gagcatggcc gaaacaaaca caagaccttc cgggagaccg
     8941 gcacccagca tcaccaggcc gggccccgga cagatgcccg caatccccca gccgatgccg
     9001 aagagtgcac tcccgacgac cagacgccgg tcgatgttcc ggagcgtcgg aatctgcaat
     9061 gcggccccga acaccgggcg tccgcgtctc agcgcgaccc ggtaccccac gacgcccagg
     9121 gcgatggcac cggccatcac gaaggccagg gacggatccc acgcgccggc cagatcgaga
     9181 aagcccgtga ccttggacgg gttggccatg cccgacacga tcaggccgat gccgaacacc
     9241 aggcccgcca gaaaagcagt gaagacgatc attacggcct cctcaagcga gcagatggcg
     9301 gacgacaaag acggtcataa agcccgtacc catgaacgtc agggtggcca ccagcgatcg
     9361 cggcgacatc cgcgacagtc cgcacacccc gtgaccactg gtacatccgg accccagccg
     9421 cgccccgtaa cccaccagca gaccggccac gaccagcccg ccccagcgcg cgtccacacg
     9481 gatctctggc aagggaagga acagcccgaa gagggctggc gccaccacca gccccgccac
     9541 gaaggcagcc cgccagctca gttcagacac gctgcagcgt tgcagcagcc cccccaggat
     9601 gccgctgatg ccggcaatcc gaccgttgag caccagcagc aggcccgtcg ccaggccgat
     9661 cagcgcgccc cccgtgaggg ccgcctgggg cgtgaaatgg gtccaatcga tgttcatgat
     9721 gttcatacgt cctttcgggg acagaactgc tcatacaggg tgtccagcag cgccagcacc
     9781 cgtacgtcgg caatcgaata gaagactcgc ttgccatcgc gccgcgtcgc caccaggttt
     9841 tcggcgcgca ggacgctcaa ctgctgggac agggttggct gatgcagatc cagctcggcc
     9901 tccagctcgc cgaccgagcg ctcgccacgc gaaagctggc acagcagcag cagccggttt
     9961 tcattggcca gcgcgctcag caccgcggtc gccgcactgg cggcctgccg gatcgcttcc
    10021 agatcgggga gggtttcggt atcgctcatg atgaacagca cgggatcgat ggacggacga
    10081 tacgccacaa cacttaaagt gtcaatatat tattgtattc taatttattg gtggcacttc
    10141 gggacacctg cccggcgcgc accaacacac ggccccctgt ccggccaccg gatcatgcag
    10201 ggcagccttg tgcccgccac atcacggcgg acatccgagc cacggcgagc ctgagcgcac
    10261 acgcaaaaac gcccagccac gtgagtgact gggcgttctg cggtattggt tgcgggggca
    10321 ggatttgaac ctgcgacctt cgggttatga gcccgacgag ctgccagact gctccacccc
    10381 gcgtcggaga aggcgaataa tagacaatct cgaagagaga gtcaagtatt tttgcggcgc
    10441 atcatcacaa gcatgcattt gcgtccccgt gaaccgtcgc ccgacgggcg cccggttggc
    10501 atcgccgcgt cgacctgctt caatggaaag cggcctcacc catgcatacg cccggcgtcc
    10561 acacggcccc cgtgcccatg ttcgccccag ccctcagacc aggaacgtcc atgccctaca
    10621 tccttcgcag cgtgctcgtg ctgaccgccc tgagcgggct cgccctgccg gtccaggccc
    10681 tcagccccgc cctggcgccg aacgcaagcg tcaccgtcga tcgggcgggt gtcttcgact
    10741 attacgtcct ctccctgtcc tggtcgccca ccttctgcct gacccacgcg gacaacagtc
    10801 agtgcagcaa ggggtacggc ttcgtgcttc acggcctgtg gccccaatat gcgcgcggcg
    10861 gcttcccgca ggactgctac tcgccggagc gcctcacacc cgaagccgtt cgattcggcc
    10921 ggacgatctt cccggccgag aacctgatcg atcacgaatg gcgcaagcac ggcacctgca
    10981 gcggtctgag cgccctcgca tacttccggg cggcggatcg cgcccgcacg cagatcaacg
    11041 tccccgcccc gctcgaaagc cccgcgacga cccattccga cactgcggcc gccatcgccc
    11101 ggtcctttgt cgccgccaac ccgggcattc cctccggcag cgtcaccgtc gtctgcagcg
    11161 gaccgcagtt atcggaggtc cggctctgtc tgaacacatc cctggcgccc gtcgcctgcg
    11221 gcaagggcgt cacccatgcc tgtcgcggcg gcccgatccg catccccagc gtgcgttgac
    11281 gcctgtggcg gtgatccgcg agggcgtacg tgtggcgccg tccatcacag aatccaccgc
    11341 gccggagcac gtgaccgact acgcgatgcc cgcctgattg ccaccggggc gggcgcgctg
    11401 atcgcgcccc ccgtcgaagc ccgccacgcg gaacgtcccg cgtcgcctgc ccggcgttgc
    11461 cccagatcga cgaagatcgc cgcccccgct ccgcacgaac cgtgttcaag gacccaccgg
    11521 acattcacgc gtcggacgcg ccggtccggg gacgcctcca gggaagcggg taccgtccgg
    11581 gctcctcggc cttgcacgaa cacgttgcgg cgatggccgc tgccctcact ggacgatgaa
    11641 ggtggtgaac agcgcgcgct tgaccgggcc ttcggcccga tgggcatgcc ccttcttgtc
    11701 ggtgtaggcg gcggtgccca ccacggcatt cacggcgtcc tggatatcgt ctgacaggtc
    11761 ctcgcgcgcc ttggtgatca acagctcggc ccccgtacgc gacgccagca gccccagaat
    11821 gtcattgcgg atctgcggca tgtaggcgga gatcgcggcg gcgcctgacg gatcgtgcag
    11881 gcgcagggca atcgtgagct ggatgaagcg cacgttgccc cccggctccg gcgccaggtt
    11941 ggtggtgatc gtctccatgt tgacgagatc ggatccgccc ccgccgcctc cgtgatcgtt
    12001 ggcccatgcg ggcaccgaca cgacgagggc gcaccacagg aaaaggcgga agaggacact
    12061 tcgggcggac ttgcacatgg gggaaactcg ccagctgcag acgtcgattg ggcagggacc
    12121 ggcatgtccg gccaccgagg cggagggccg cacgtctcca gcaccttccg ctccgactat
    12181 taacggacaa tgaccgccgc gccttgaagg gctacccaga atgggcagca gacaggttcg
    12241 tctgcgctgc acatctgata actttcgtgc cggaatggac ggcgctcatc gtccccggta
    12301 cgcccggcag agcccccgga tgcacgccgg atcatgcagg atagatgaac gggagagacg
    12361 cccgaaaggc gttcagagcg cggaataagc gggtgccggt gaaaggaatc gaaccctcga
    12421 ccttcgcatt acaagtgcgc tgctctacca actgagctac accggcaggt actccagaac
    12481 ccatgtcccg ccttgtgcgg cggcatggac gggcgaccga ggcctgctct atgctttcca
    12541 cctcagcagg cccgaacgag ggccggatta taaacaaagc gtccccaata cgcagcacac
    12601 ggttccgtca tgcccgacgc cccccagaca gacgccctta cggaaggcgg tgacgcgccc
    12661 ggccagccta tccgcctgct gttcatcgaa gactccgaga acgacatgct cctgctgctg
    12721 catcacttcc gcagcgcagg ctacgccccg catttccgtc gggtcgcttc cgaagccggc
    12781 ctgctcgacg ccctcgccga cacgacctgg gacgtgatcc tgtgcgacca caaactgccg
    12841 ggcttcagtg cccagggcgc cctgcgcctg atccaggaac gcgccctcga tctgcccttc
    12901 atcatcgtct ccggcgtgat ccaggaggat gaagccatcg ccgccatgcg cgccggtgcc
    12961 cacgactacc tcagcaaaag caagctcgac cgcctgattc cggcggtgga gcgggagctg
    13021 cgcgaagtct gcaaccgtcg cgagaagcgc ctggccgagc agaccctgcg ggaaagtgag
    13081 gcgcgccagc gggcccttac cgacaacatc cccggcgtcg tcttccgcat gggcttcacg
    13141 tccgacggca tgcttggctt ccagtacctg agcgaagccg ccagcatgct cttcggcacc
    13201 tcggcggacg aactcatcgg tggttccgcg ggctggatga cctggctcct cgacgacgac
    13261 ctgccgggct tcatcgaggc ggcagacctg tccgccgcgc acctgtccac cctcaactgg
    13321 gaaggccgca tccagctctc ttccggcgag cagaaatgga tcaacctgcg cagctcgccg
    13381 cgcctgtccg aggccggcga agtggtgtgg gaaggcgtca tgtggaacat cacccacagc
    13441 aaacgggtcg aatccgatct gagggaatcc cgcagccagc tcgccgccct gtcgaaccac
    13501 ctgcagcgca tcaaggaaga cgagcgcgaa cgcattgcgc gggacgtcca tgacgtgctc
    13561 gggggaaccc tggtggccat gaagttcgag atgtccctgc tggaatccaa gctggaatcc
    13621 catccggctc aggcgcggga gcgggcacgg aacgtgggcc gcatggtgga cgacgccatc
    13681 gccaccgtcg gccgcgtcac ccgcgaactg cgccccggca tcctgaagga cttcggcctg
    13741 cctgccgcca tcgaatccca gggcgaggac ttcgcccagc gcaccggcat ccaggtcaag
    13801 atcctctgca acgaccacga cctggcgccc gactacgaca ccgcgctggc cctcttccgg
    13861 gtcttccagg aagccctcac caatgtcagc aagcacgccc gcgcgtcgca ggtcggcgtt
    13921 cgcctgctcc aggaaggcga tgaggtgctc cttgagatca gcgacgacgg cgtcggcatc
    13981 gacgccgccg acctgcagaa gccccgctcc ttcggactgc gcgggatccg cgaacggatg
    14041 agcagcctgg gcgggcgcct cgatgtggat ccggtcctgc cccacggcac ccggctgacc
    14101 ctgcgcgccc ccttgtacga ccccaccgcc accgagcgcc ccccccaact ccggagtcat
    14161 gcgcgatgac caaaaccatc cgagtcctga tcgccgacga tcacgccatc gtccgccagg
    14221 gtctgcgcca gatcctctcc gacacgcccg acctcaccgt cgccggcgag gccgagaacg
    14281 gcgtccaggc cgtacagatg gtgcgcaacg gcgaatggga cgtggtcctg atggacgtct
    14341 ccatgcccga tcgcaacggc atcgacgccc tcaagctgat caagaaggaa ttcccccgcc
    14401 tgccggtgct gatcctcagc atgtacccgg aggaacagta cgccatccgc gccctaaaat
    14461 ccggggcggc cggctacctc accaagcaaa gcgcgccgga actcctcgtt accgccatcc
    14521 gccaggtggc cagcggcaag aagtatgtca gcccgtccct cgccgaagag ctggtcaacg
    14581 cgatcggcga ggacaccgag cgccccgccc acgaaaagct ctccgaccgg gaataccaga
    14641 ccctgtgcat gatcgcgtcc ggcaagacgc ccaccgagat cgccgaggcg ctcaacctgt
    14701 cggtcaagac cgtcagcgtg tatcgcgccc ggctgctcga aaagatgcat ctgcgcaaca
    14761 atgccgaact gacccactac gggctcaagc acgggctcgc ggaataatcc gccggcgtac
    14821 tcaagaacag ctcaagggcc gcccgccggg gccgttatag catccggatg caataacctc
    14881 ccctcttaca ggtcactcat tcgtgggctc gtcaggcgag ccaccggcac gccggatgat
    14941 ggaaaaccgc ccccccgctc cgcgccgctc caagatgatc gacaccagcc agccgtccga
    15001 tctcgcccgt gagacgctcc ggcaactggc gttgcgcaag attgcgccca cgccggacaa
    15061 ctatcgggcc ctctaccacg aaatcgccgg cacccaggcc gaagacggct ttcccgaccg
    15121 catgctgcga cagatcggag cagccctgcc acgtcattcc cgcgacgccg tgcgcttcgc
    15181 ccaggccttc gaggatgcca ccgcgcgcca ggactggcag gccgtcaaga cctcgctgac
    15241 cgaggccttc accgccctcg acgtacccga actcaactgg ccggcgctgc tgcgcgagct
    15301 catcaccgaa tgggaccggc gtcaggccgg gctgacccag gcccgcaagc gggagatgct
    15361 cgaccgcgtc ctgtccgctt ccggtgccca tcccgagcga ctccacgaac gcctgcaagc
    15421 gctggtgcgc gcctgggccc gcaacagcga cgacgacagc accgactcca cggctgccac
    15481 cgaatccggc gaggtccgcg cgcaggccga acgcatgacc agcgacacgc cggtgccggc
    15541 agacagctcg ggggtcgaac tgctccagcg gctgctgacc ggcctggagg cggtcgtcgt
    15601 ccatctcgac ccggaagcgg cccacgaact ccaggccatt gccgcgcacc tgcagccggg
    15661 cgccccgtca gccccggtcg gtgaactgcg cacccgcctc gaccgcctcc tgtcgcacct
    15721 gaactgggtc gccgaggatc acgccgccct gcgcgacgcc ctcctgcggg tcctcaagct
    15781 gatcgtggac aacatcagca agctggtgat ggacgaccaa tggctgcgcg gtcagatgag
    15841 cctgctggac gaagtcttca gccacccgct ggacatccgc accctgggcg agctggaatc
    15901 ccgcctgcgc gacgtgatcg cccggcaggc cgccctcaag cacgaactgt ccgacgccca
    15961 cgccaagctc aaggacatgc tggcccgctt cgtggaccgc ctcggagaat tctccgactc
    16021 caccggcgac taccacgaca agatcgaaca gtgcgctggc cgcatctcca ccgcccgcga
    16081 catctccgaa ctgaccgacg tcatcgccga agtcatgaag gagacccgca gcatccagga
    16141 atcgaccctg cgctcgcgca ccgaactgga ggaactgcgc ggccgcgtcg agaacgccaa
    16201 tgtcgagatc aaccgcctgc agaacgagct cgagcagacc agcgaactcg tccgccacga
    16261 cccgctgacc ggcaccctca accgcaaggg cctggacgac gccttcgccc gcgagaccgc
    16321 cctcgcccgg cgccgcaaca cgcgcctgtg cgtggccctg ctggacgtgg acaacttcaa
    16381 gcacatcaac gacacccacg gccaccagac cggcgacgag gcgctccagc acctcgccgc
    16441 ggtgatccgc gagaacctcc gcccgcagga ttccgtggcc cgctacggcg gcgaggaatt
    16501 cgtcatcctg ctgcccgaca ccgacgtgga cctcgccact gccaccctcg tgcgcctgca
    16561 gcgcgccctc accaagcgct acttcctcgc ccgccagcag aagctcctca tcaccttcag
    16621 cgctggcgtc gccgaactgc aggccgacga agacaccgaa agcgccctcg accgcgccga
    16681 caaggccatg tatatcgcca aacggtccgg caagaaccgc gtcatctccg ccacctgagc
    16741 ccccgcccga catcgggcac accccgctac acgcgcatgg aatatgctag atttctggca
    16801 tgcgcccctt cgcctgctgc ccctcgcctg cgcccagcgt cctgaccggg cccgctgtgc
    16861 tcgtcgccgt cctgctgctc accgccacac cagccgatgc cgagccctcc ggacgccttt
    16921 tccacacccc cgaggaacgc cagcgcctcg accgcctcct cgacaacgcc gcctccggcc
    16981 gcgcccagcc tgaacggctg gacggcatca tcacccgggg cgacgggcgc acgaccctga
    17041 tcctcgacgg acatccacgc cccgctgccg ccgatctccg gcccttggac gcacggtccg
    17101 cccgcatcct tgccaacgac ggccagaccc accgcctgcg cgtcggcgag cgcctgccgc
    17161 ccctgtcggt gccttgaacc gtctggccgc acgtcgccac cgttcgcagg gcatggtcgc
    17221 cctcgcgctg gtcgtcctcc tcgccctcgg tgccaccggc tggttcctcg cccgccggga
    17281 cagcaacgca gctcgccacc tcgcccgcga acagcgcacc gccgacgccc tgcgcaccgc
    17341 ccgtgacgcc ctcatcggct ttgccgccac ctatcgcaac agtctccacc cggacgccga
    17401 cttcggctac ctgccctgcc ccgacctgga cggcgacggc tcgtccgaaa cctgtggcgt
    17461 gcaaggcgac acccgcgtcg gtcgcctgcc caccctcacc ctcggcctgc cggacctgcg
    17521 cgacggggac ggcgaatgcc tgtggtacgc cgtcgccggc tccttcaaga acaacccgaa
    17581 ggccgacatc ctcaatccgg acagcaccgg acgcttccag ctcctcgacg cggccggcgc
    17641 gccccgcagc ctgcccggcg accaggccgg actggccgcc gcggtcattt tcgcaccggg
    17701 cccgcccctg cccacccaac agcgccagag cagcaccggg cgctgcggcg gtgacctctc
    17761 agccagcgcc gtgggtgcct acctggaatc gctgcagccc ctctctggcc ccggcgttct
    17821 gaccgtcgat ccgcgcggga gcaccggtaa cgaccgcatc gtctggatca ccggcgacga
    17881 catcatgcgt gccctcaagg cccgcgccag cttcgccacc cacttgaaca ccgtcctcga
    17941 cgccaccgcc gactgcctgc ggcgggccgg cgtgcctgca ccgacgcgca ttgaatccac
    18001 agccaacggc ctccagcttg gacgcgttcc ggctttcagt ggcaatgccg gcgcctgcac
    18061 cagtgccggt ctgcgggacg ccgtcgagaa ctggggcgaa ctcatgcgct acgcccgctg
    18121 taccgacggc acggcctgcc tgagcagcgc cacgggcccc tgtcgcggcg ttctcctgtt
    18181 cggcggcgaa cgcctgcccg ccggccagcc ccgcgtgacc gacgccgaca aggcgctggc
    18241 cgcccactat ctggaatcca ccaccctcgc cgccctgtcg gcccggcagc tgggcggcct
    18301 gcccagccgc atcctcatcc ccttcgcgcg ccgcagcgag ccggccagcg ccgacgtggc
    18361 cgtgtgcctg ccctgaaccc gcccaatccc gttcgatccc gtccgaccat gaccgaccga
    18421 ccctcccgct ccagcgcccg cggcttcacc ctcgtggaga tggccgtcgt cctgctcatc
    18481 ctggccatcc tggcgggcgg cctcaccgcc ggcctgtcgg cccaggtggc ccgccgcaac
    18541 gaagccgaga ccgatgccgc gctggacgag gcccgcgacg ccctgctcgg ttacgcgctg
    18601 cgccgtggaa cgctgccctg ccccgccagg agtggcagcg acggcgccga ggatcgggaa
    18661 agcctcacca cctgccgtgg caatcgcctc aacggcctgc tgccctgggc cgaactgggg
    18721 atttccggcc tcgatgcctg gggcaggcgc ctgcactatg ccgtcgcgcc ggactacgtg
    18781 aaggccatca ccctcgacag cgccggcaaa ctggacatac gcacccgcaa cagcgccggc
    18841 accgagctca ccctcaccac cgccatcggc gccgcgcccg ccctggtcct gtcccacggc
    18901 cccaacggcc tgggcggcat cgacgccgac ggcaacgccc tcgccgcacc gccggcgggc
    18961 tccgacgaag cccgcaatgc gaccgccggc agcaccctct tctacgcccg cagcctgtcc
    19021 gaaaattccg atgctgccgg tggcgccttc gacgaccggg tggtgtgggt gtcgccctac
    19081 ctcatcgcca accgtctggc gagcgccggc cgtttgccct gaagccggca caccgcattc
    19141 catccgaata aaacacaaaa ttcgccagca cacgaaacac atttgcacga atatcgcgac
    19201 tttgagggtt gaccgcggta tcgccccctt ggacactgcc gccactcgac tcacgcagga
    19261 tccgccatgt ccgtctccgc ggtccatcgc ctgctggccc ggctggcggg cgttccccgg
    19321 ccgtcttccg atcaggagga cgcatggctc gaacgcacct tcttcgtcct cacgggcctg
    19381 ctcacggccg gcgcaagcgc actttccgaa ctgttccgcc aggtggccgg tgcgggcacg
    19441 tcaccaggca tcaccgtgtt ccttgttctc ggcatcggcc tcaacctcgc cgcgagccgg
    19501 cgccgcgccg acttccggcc ctgtgcccac gtccaggcca gcctgatcct cggcgctccg
    19561 ttcctcgcgc acctgctgca gaccgacctc atcggcgacg ccggccgcct gccgtgggcc
    19621 atgctggccc cgctcggtac cctgctgtgc ctggatgccc gcgcctcccg ccgctggttc
    19681 gtgaccctcc tgggggtgac cgccggcacg gtggccttcg accagagccg cctcccgcgc
    19741 cacccgggaa tgcccgatgg cgtgaccctc ctggccgtcg ccgtcgatct caccctgctg
    19801 gccgggatgc tctatttcct gctgcacttc gccgtcaccc agaaggtcca gacccgccag
    19861 cgcctgcgcg ccgcccaccg acggctgcgc gacgagcagc agcatgcgga aagcctgctc
    19921 ctcaacatcc tgccagcacc catcgcggcg cgcctcaagc tcagccgcgg caccctcgcc
    19981 gacggccacc ccgaagtcat cgtgatgttc gccgacatcg tggactacac ccgcatcgcc
    20041 agcggcatgc agcccaccga ggtcttcgag atgctcaacc gggttttctg ccgcttcgac
    20101 gaactggccg aacatcacgg cctcgaacgc atcaagacca tcggcgacgc ctacatggtg
    20161 gccggcggac tgcacgccca ccccaacgaa ccccacgccg ccatggccgc catggccctc
    20221 gacatgcagg ccgccgtgcg cgaccacgcg gtgaccggcg gcatcacgct ggagctgcgc
    20281 attggcgtcg cctccggccc cgtcgtggcc ggcgtggtgg gacggcgcaa gttcatctac
    20341 gacctgtggg gcgacaccgt caatgtggcc catcgcctgt gcaccgaagc ccagcccagc
    20401 cagatccagt gcgacagccg gctctacgag cgggtgcgcg gccgcttcgt gttcgcggcg
    20461 ccggtgctgc tcttcctcaa aggcaagggc tacgtgcccg tctaccggct gcgcagcccg
    20521 cgcgtcctcg ccgtgcatcg cgagccgccc catccgagcc accgcgccgc ctgagcctcc
    20581 gcgcagacgg cacccgcaca accacactcc cccttccgcg ccgaccgcgg gatgccctag
    20641 aattccggct ggcataattt atatgcatgg atgcatccac acgtccatac accaacggaa
    20701 acggggagca agatggcgca ttctcaactc accagcagac agcgaggctt caccctcgtc
    20761 gaaatcgccg tcgtgctggt catcatcggc ctgttgctgg gcggcgtgct gaaggggcag
    20821 gaacttatca acagcgccaa ggtgaagagc ctcatcaacg acttccgcaa cgtcgccacc
    20881 ttcgcgtacg cctaccagga ccgcttccgg gccctgcccg gcgacgatgc gggcgccacc
    20941 agccacctgt ccggcggcgt caaggcaagc accggcggca ccgccggcaa cggacgcatc
    21001 gaaggcaact ggaactccac caccgccacc gacgaatccg tgctgttctg gcagcatgtg
    21061 cgtctcgcca acctggccag cggcgacggc cgcaatccgg gcctcgaagg actggtgatc
    21121 ctcgactggc tgccccgcaa cgccgccggc ggacgcaccg gcatcaccgg cgtcgcgcca
    21181 atcaccggct gggcgggcaa cttcttcgtg tgtcaggaca acatcccggg cagcctcgcg
    21241 cggcaagtgg atatcgccat ggacgacggc cagcccgccg ccggatcggt ccgtctgctc
    21301 gccaatggca gcgcctcggg cagcgccgtg gccctcgccg atctggccga cggcacccct
    21361 tacaccgtct gcgcagccct ctgatcagag gcccttcagc aggcgcgtct cggtgttggc
    21421 caactgggcc ggcgtgccca gcagcaccag tacatcgtcc agttccaggc gggtttccgg
    21481 cgtcgggtcc accacgcgga tgcgtttgcg ccgcagggcg ctcacctcca cccccaactc
    21541 gcgcagatgc agctccgcga gggtccgccc gatggcatag gcgccggcct ccagggtcag
    21601 ggaatgcagg cgcgcctgct ggcgttcctc cacggcctct tcgtgcaggt gggcaccccg
    21661 gtagatgccg cgcagcaggt gatagcgctc tgcgcggatt tcgcggatgc gcttgaggat
    21721 gcgcggcagg ggcacaccga gcagcgccag cgcgtgggag gccagcatga tcgacgactc
    21781 caccgattcc ggcaccacct ccgccgcgcc cgcctcctgc agcttctcca gatccacctc
    21841 gtcggccgtg cgcaccacca ccggcacgtc cggccgcacc gaacgcacct ggctcagggc
    21901 gcgcagggcc gcgtccgtat ccgcgtaggt caccaccacc accgaggccc gcgcgatgcc
    21961 cgccgccacc agggtctcgc gcttcgccgc atcgccaaag accaccgtct cgccggccgc
    22021 cgccgcctcc cgcacccgct ccgggtccag gtccagcgcg atgtaggtga tcccctcctg
    22081 ctccagcaga cggcccaggt actggccatt acgcccgaag ccacacagga tcgcgtgctt
    22141 gtccacgaac atcgactggg tcgcgatgct cgtcagctgc agcgagcggc tcatccactc
    22201 cgacggcacc aggcgcagca cgatcttctc gctggcctgc acgatcagcg gcgccagcag
    22261 catggacagc accagcgccg ccaccaccac ctgcatcagc gatgtcggca gcagctcggc
    22321 gccggcgatc tccgacagca gcacgaagcc gaactcgccc cccgcgcaca gccacaggcn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgatctccga cagcagcacg
    22501 aagccgaact cgccccccgc gcacagccac aggcccgtgc gcaccgccgt cccctgggca
    22561 ctgcccatga tgcgcgaggc cgccgccacg atcacgaact tgcccaccag caagagcgcc
    22621 agcatcgcca gcaccgccgc caggtgggcc acgatctcgc ccacgtccag gaacatcccc
    22681 accgtcacga agaacaggcc cagcagcacg tcgcggaaag gcttgatgtc ctcctccacg
    22741 tggtagcgga actccgtctc cgagatcagc atgcccgcca ggaaggcccc cagcgccatc
    22801 gacagccccg ccgactccgt catccacgcc agcccgaggg tgatgaacag cacgttgagc
    22861 atgaacagct ccggcgaacg ctgccgcgcc acatggaaga accacccgcg catgagcttc
    22921 tgcccgaaga acagcaccag cgccagcatc cccaccgcct tggccgtcgc cagtcccagc
    22981 gtcagcatca gcgcctccgc gggccccgac agggcgggga tcaggatcag cagcggcacc
    23041 accgccagat cctggaacag cagcacgccg atggtctcgc gcccgtgctt ggaatccagc
    23101 tccagacgat ccgacagcag cttggagagg atcgccgtgg acgacatggc cagcgtgccg
    23161 cccaacgcaa agctcgcgcc ccaccccagg ccggcgagcc atcccaccac cgtgacgaaa
    23221 gccatcgtcg ccagcacctg aaaggccccc aggccgaaca cgatgcgctt catcgcgaac
    23281 aggcgcggca gcgagaactc cagcccgatg gagaacatca ggaagaccac gccgaactcc
    23341 gccagatatc gggcgccgcc cgtgtcgggc atgagcttca tggcatgggg ccccaccgcg
    23401 gcgccgacca tcagatagcc caggaccggc ggcaggttca tgctgcggaa caggccgacc
    23461 acgaagaccg cgccggcgag caggatcagg accagttcga gggtattgac catgggtgag
    23521 gcgtcggggg gcccggaagg ggtgccgggc cgttctgtta tactccgttg cccgattata
    23581 cccacgccgt ccatgacacc gaccccgcat cccgccgcgc ggccccaggc cgtggaactc
    23641 gcccgccggg tcctgcgcat cgaggccgat gccgtggccg ccctcgccga gcggctcgat
    23701 gcggtggcct tcacccgtgc cctcgacctc atcctcgcct cccgcggccg ggtcatcgtc
    23761 tccggcgtcg gcaaatccgg tcacatcggc cgcaagctcg ccgccacgct cgccagcacc
    23821 ggcacgccgg cctact
//
LOCUS       DAOQPA010000046        23777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1010_length_23777_cov_18.606863, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000046 DAOQPA010000000
VERSION     DAOQPA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23777
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_23777_cov_18.606863"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1182
                     /locus_tag="PKX01_08320"
     CDS             319..1182
                     /locus_tag="PKX01_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76167.1"
                     /translation="MTDLNDLIARAGQLLDRLEGLLPAAPTAPDWHAAVAFRWRRSRS
                     AATLQPVLRPHAIRLDDLQDIDDKKARIVANTRQFLAGKRANNVLLTGARGTGKSSLV
                     KAVLNEFADAGLRLIEVDKDDLIDLPDIVELVQGRPERFILFTDDLSFEDGEPAYKAL
                     KSVLDGSVAAVPDNVLIYATSNRRHLMPEYFAENAATKHKDGEVHPAEAVEEKISLSE
                     RFGLWISFYPFSQDEYLAVVNHWLREFGVPAEAVADARGEALQWALLRGSRSGRVAWQ
                     FARDYAGRFDA"
     gene            1179..2132
                     /locus_tag="PKX01_08325"
     CDS             1179..2132
                     /locus_tag="PKX01_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HMZ76168.1"
                     /translation="MSASPPRKIVHVAAAVITRPDGSYLLGQRAPGTFYPGYWEFPGG
                     KVEAGETPRDALVRELAEELGITVETAWPWIVREHVYEHAHVSLHFFEVPAWHGELTD
                     KVHSALAWQHPDAVDVTPVLPANGPILKGLRLPRRMGITQAGAVGVERQLKRVDAALA
                     AGLRLVQIREPELDAEMLACFAREVVARCHAVGALAVLNGDAELAHTLGADGVHLPAR
                     CLMSLAARPDLDWVGASCHTRAELERAAALGLDYAVLGAVQATATHPERKPLGWSGFT
                     TLAAGLPLPVFALGGVQRDDLETARAAGAHGIAAIRAAWDT"
     gene            complement(2172..2372)
                     /locus_tag="PKX01_08330"
     CDS             complement(2172..2372)
                     /locus_tag="PKX01_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HMZ76169.1"
                     /translation="MSTPVRSREVKCPTCGKAVLWVPENAFRPFCSERCRKIDLGAWA
                     SDTYRVAGETPLDDGADPLTRT"
     gene            complement(2369..3124)
                     /gene="zapD"
                     /locus_tag="PKX01_08335"
     CDS             complement(2369..3124)
                     /gene="zapD"
                     /locus_tag="PKX01_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HMZ76170.1"
                     /translation="MISYEYPLNERIRTLLRLEDLFDKVMHFVRADGSLEHHVALVTL
                     FEILEVASRADLKVDLVQELERQRQILLSFRNNPQISEEALSGALYEIEQASASLLAM
                     AGKTGQYLRENEWLMNIRSRAAIPGGACEFDLPSYHHWLNRDVEFRRRDLVAWVQPLL
                     PIRDGLAIVLRLLRASGRPEELVAARGAYQQTMGGRTVQMIRIRLSSGDPYVPETSAN
                     KYALNIRFMHAETVVRPRQSEADVRFELTFCNL"
     gene            complement(3178..3801)
                     /gene="coaE"
                     /locus_tag="PKX01_08340"
     CDS             complement(3178..3801)
                     /gene="coaE"
                     /locus_tag="PKX01_08340"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938351.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMZ76171.1"
                     /translation="MSSGPFVVGLTGGIGSGKSAVADRFATLGAAVVDTDRIAHALTA
                     PEGAAMPALRAAFGEGIVAADGALNRAAMRALAFEDASVRKRLEAILHPLIRDESARQ
                     CAVAVAPYVVLVVPLLIESGTYRERVQRLCVVDCPEALQVERVMCRSGLDERQVRAIM
                     AAQATRAARLAAADDVVDNAGSLEALQARVAALHGEYIQCAARGRVL"
     gene            complement(3798..4538)
                     /locus_tag="PKX01_08345"
     CDS             complement(3798..4538)
                     /locus_tag="PKX01_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ76172.1"
                     /translation="MVPLRVAVIVLIFANLLALALWKGWLGGGDSDGQPERLSNQLNA
                     ERLKLAVDARPPAPRPREAIAEAPRTSPEPPVPTPAAPAQRESAPATDTSAPPACVAF
                     AGLGVDQAQELAARIGKVGGGLKLNESRSEQPSSWWVYIPSPGSKDGADRKAAEVRKL
                     GVDDLYIVPDAGPNQFAISLGLFKSEVAANRQLEVLRAKGVRSAQIATRGSALVRLEV
                     RGPSDSLATLVSDLSDHVKGVSRQGCKP"
     gene            complement(4594..5313)
                     /locus_tag="PKX01_08350"
     CDS             complement(4594..5313)
                     /locus_tag="PKX01_08350"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMZ76173.1"
                     /translation="MNLLIDAGNTRVKWGLHDGAVWHARGAVPTATPEALEAALAVQP
                     VPARVMGANVAGASVALAVGRLCAAWKRTPGWLVPGIEAGGVRNAYLDPTQLGPDRWA
                     ALIGARTLHAGAALVVTAGTATTVDVLTAEGVFRGGIILPGLELMRRALARDTARLPF
                     ADGRFDATPRRTADAIVSGCLHAQAGAIERMFRLVADAPDAVCLLNGGAAADLGPQLG
                     CPVRQVDNLVLEGLARLTSGK"
     gene            complement(5310..6116)
                     /locus_tag="PKX01_08355"
     CDS             complement(5310..6116)
                     /locus_tag="PKX01_08355"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMZ76174.1"
                     /translation="MHSLSCEPLDRDAVLAVLGDHAAAFSVVVVPECASTNSVLLDDP
                     PADDGRIHVLVTDRQTAGRGRRGRNWLSWPGAGLTFSVLWRFEPGAPVPAGLSLVAGL
                     AVARALEELGVEGVQLKWPNDVLIHGRKLAGILVELIPGRGRTPAAVIGVGLNLRLPP
                     EADIPHAGGVTDLAACLTAPPTRARLLGCLLDQFRGLLELYAAAGFPALRGAWQQRNA
                     HAGLPVRILGETVSQEGTCVGVDEDGALLLNTGSGLQRVLSGEVSLRSVA"
     gene            complement(6341..7744)
                     /gene="ntrC"
                     /locus_tag="PKX01_08360"
     CDS             complement(6341..7744)
                     /gene="ntrC"
                     /locus_tag="PKX01_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HMZ76175.1"
                     /translation="MSAVWIVDDDRSIRWVLEKALAREEIPYKSFGNANDVLTALDSA
                     AQPPRVLVSDIRMPGESGLTLLSKVKARFPHLPVIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQAVELVRRAIDETAHQHAGEEVLSAVPEILGQAPSMQEVFRAIGRLSQSHA
                     TVLINGESGTGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGA
                     QTMRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGNFYRVGGHQPIKANVRVIA
                     ATHQNMEERVRQGLFREDLFHRLNVIRLRLPPIRERREDIPMLAKHFIALSAQELGVE
                     PKRLSDATLKYLQSLDFPGNVRQLENLCHWLTVMAPGQTVEVADLPPELKEQPTREQP
                     ANWVDALTAEADRALVQNPGEVFDRLSRDFERILIRRALNATGGRRIEAAQLLGIGRN
                     TITRKIQELGLEEGEHA"
     gene            complement(7821..8894)
                     /gene="glnL"
                     /locus_tag="PKX01_08365"
     CDS             complement(7821..8894)
                     /gene="glnL"
                     /locus_tag="PKX01_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HMZ76176.1"
                     /translation="MQNDPPDQTPSPFAGLELLSSAVVLVDDELIVRYLNPGAENLFA
                     VSSRKIVGYSLRRLLGEPAGLGTALNNVLRDNWSYTGHNLTVLRPGMEALHLDCTVTP
                     VESDAVRLLLEFRPMDAHLKVAREEQLAHQQQANRELIRNLAHEIKNPLGGIRGSAQL
                     LERELDDPQLREYTQVIIAEADRLQDLMNRLLTSHRMMQPSQLNVHDVLERVRRLVLA
                     EFPSVEVRRDYDTSLPYVTGDREQLIQAILNITRNAAQAMNGDGEIVLRTRAARQITM
                     AKRRFKLALQLQVIDNGPGIPAEIRDKIFYPLVSGRENGSGLGLSLAQSFIEQHQGVI
                     DVDSRPGRTCFTIFLPITRDSDV"
     gene            complement(8972..9472)
                     /locus_tag="PKX01_08370"
     CDS             complement(8972..9472)
                     /locus_tag="PKX01_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HMZ76177.1"
                     /translation="MKLPVVLGLAALLSAAPALADIFKCIDEGGHVTYTNAKVGGKGC
                     SLLSRDQPVSSMPAGPASRPAGAPSTAPAAPSASGAAGFPRVDAGTQRARDNDRRRIL
                     EDELATEQNSLAVAQKDLADQEAIRNGDERNYQRVLDRLQPFKDKVQLHERNIEALRR
                     EIANLR"
     gene            complement(9639..11048)
                     /gene="glnA"
                     /locus_tag="PKX01_08375"
     CDS             complement(9639..11048)
                     /gene="glnA"
                     /locus_tag="PKX01_08375"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HMZ76178.1"
                     /translation="MTPQDVMKMVQENEVKFVDFRFTDTKGKEQHVGVPLSAFDEDKF
                     ESGHAFDGSSIAGWKGIQASDMLLMPDPNTAYIDPFFDETTLVLTCDLIDPADGKGYD
                     RDPRSIANRAEAYLKASGIGDTAFFGPEPEFFIFDSVEWKVDMSGAYHKIFSEEGAWS
                     SDIKYEGGNTGYRPAVKGGYFPVPPVDSFNDIRAAMCLALEACGVPVEVHHHEVANAG
                     QNEIGTKFNTLVRRADQTQILKYIVQNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNLFAGNGYAGLSETALYYIGGIIKHAKALNAITNPLTNSYKRLVPHFEAPVKLA
                     YSAKNRSASIRIPFVNSEKARRIETRFPDPGANPYLCFAALMMAGLDGIQNKIHPGDP
                     ADKNLYDLPPEEDALIPTVCSSLEQALEALDKDREFLTRGGVFSNDFIDSFIALKNDE
                     VNRLRVEVHPVEFEMYYAI"
     gene            complement(11244..12422)
                     /locus_tag="PKX01_08380"
     CDS             complement(11244..12422)
                     /locus_tag="PKX01_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ76179.1"
                     /translation="MAHDSPLSAATEAPLDADRARQAQQATWVSIAVNVVLTVLQIAV
                     GLFAHAQSLVADGMHTLSDLLGDFMVMVASRRGSTPADERHPYGHGRYETAASMILGL
                     VLAGVGAGFLLSAASRLQSTEALPALHPAALWMALLTLAGKEGLFRYMLAVAERLRSP
                     MLVANAWHARADAASSLVVAVGIGGSLLGYRFLEPLAAALVGFMIIRMGLQLSFDALR
                     ELIDTGVAPEEVASIRRAVEETPGVLGVHDLRTRRMAHRILVDAHVRVDARISVSEGH
                     RIAELARHRVRSRVEDVLDVLVHVDTEEDEELAAGRHLPSRDVLLAEIAEILGPDLPQ
                     TERIILHYVGKQVEADVFLPHVWCCDARRIDALRQQVADGVARRPHWGRIALLCQIAP
                     "
     gene            12486..12938
                     /locus_tag="PKX01_08385"
     CDS             12486..12938
                     /locus_tag="PKX01_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ76180.1"
                     /translation="MGKLSETLALAQQRSRDAGLPYAGLLTPREAWDLLQAAPGARLV
                     DVRTRAEWDWVGRVPGAVELEWMLYPGNEPNPHFLAQLQRQVDPEALVMFLCRSGVRS
                     NNAATLAAGHGYTNAYNILEGFEGDKDAHGHRGKLGGWRFAGLPWIQG"
     gene            12965..14065
                     /gene="nadA"
                     /locus_tag="PKX01_08390"
     CDS             12965..14065
                     /gene="nadA"
                     /locus_tag="PKX01_08390"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMZ76181.1"
                     /translation="MQTASISFDRFNALADADAHARIRAARARLGDKAVLLCHHYQRA
                     DVYQYADITGDSLKLSRLASDTDAEYIVFCGVHFMAEVADIMSRPQQIAILPDLAAGC
                     SMADMANLAKVERCWRELTEVLDTPDALITPVTYINSAADLKAFCGEHGGIVCTSTNA
                     PVILDWAFAQREKVLFFPDQHLGRWTGFNKGIPLDEMVVWDPDLEYGGLTAEQIRKAR
                     ILLWKGHCSVHQMFQPAHILKFRNQHPDGKVISHPESALEVCRQSDFVGSTEYIIKVV
                     KESAPGTRWLVGTELNLVNRLAEEVKHEGKIVQFMAPTVCMCSTMQRIDPQHLAWTLE
                     NLAEGNVVNRITVPAHEAALAKVALERMLAVS"
     gene            14147..14896
                     /locus_tag="PKX01_08395"
     CDS             14147..14896
                     /locus_tag="PKX01_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ76182.1"
                     /translation="MSTLRICTYNIHKGFSQFNRRLAIHDVRERLHQLGADVVFLQEV
                     QGQHLHHARRHTDWPEQPQHEFLAGDLWRDTAYGGNALYDHGHHGNAILSRHPILSRA
                     NQDVSDHRFERRGLLHCEIRLSGVARPVHCVCVHLGLTAGTRRRQMAALVARLNVLAP
                     PTEPLIIAGDFNDWRNHADDHLIRQLGVVEVFTALRGRPARSFPCHLPMLRLDRIYVR
                     GFAIDQAEVHFGAPWNRLSDHAPLTAQLRAH"
     gene            14906..16066
                     /gene="clsB"
                     /locus_tag="PKX01_08400"
     CDS             14906..16066
                     /gene="clsB"
                     /locus_tag="PKX01_08400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HMZ76183.1"
                     /translation="MAIHWLPGNALTLLENGAEFFPALQAAIDTACHEVYLETYIFAD
                     DVTGRAIAAALARAAARGVTVRLLVDGFGGREFVERLRDDLIARGVHVLIYRRDISPF
                     AIRRRRLRRLHRKLAVIDGRVAFVGGINILSDLSEPNMAAPRHDYAVRVEGPLLAPIH
                     ATVQHLWELVAWASFRHRHRLPAGPAPCCGTCGDVEAAFLIRDNLRHRRDIEDAYLLG
                     IAAARREIVLANAYFLPGRRFRQALLDAARRGVRITLLIQGLADHPLQQRATRALYGQ
                     LLAGGLRIVEYHRSYLHAKVAVVDERWATVGSSNIDPFSLLLAREANVVIRDAAFATH
                     LRDHLQDAIAHGGREISADAWQRGPRLQRLLNWAAYGLVRLMIGLAGYGGRH"
     gene            16128..16616
                     /gene="rfaE2"
                     /locus_tag="PKX01_08405"
     CDS             16128..16616
                     /gene="rfaE2"
                     /locus_tag="PKX01_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMZ76184.1"
                     /translation="MNYPAPAFEAKICHPQDLAARVAALPRPLVFTNGCFDILHRGHV
                     TYLAQARALGAAMVVALNSDASVRRQGKGDDRPINVLEDRLAVMAALDCVDLVTWFDE
                     DTPIARILDCQPDVLVKGGDWPVERIVGAPETFARGGHVHSIPFEHERSTTALLTKIR
                     RL"
     gene            16727..17659
                     /gene="cysK"
                     /locus_tag="PKX01_08410"
     CDS             16727..17659
                     /gene="cysK"
                     /locus_tag="PKX01_08410"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HMZ76185.1"
                     /translation="MRIASNVAELIGKTPLVRLNALAPANGATLALKLEFFNPAHSVK
                     DRIAAAMLDAAEAAGKIKPDTIILEPTSGNTGIGLAMVCAARGYKCAFVMPETMSRER
                     KLILKAYGAELILTPGAEGMPGAVKRATELAAADPRYFIPQQFENPANPAIHRATTAE
                     EIWADTDGQVDIFVSGIGTGGTITGVGEVLKERRPGVKVIAVEPAASPVLSGGARGPH
                     PIQGIGAGFVPAILNTAVYDEIVQVPNDAALDTARRLASQEGLLVGISSGAAVWAALQ
                     VANRPENAGKLVVVVIPSFGERYLSTVLYQHLEV"
     gene            17746..18906
                     /locus_tag="PKX01_08415"
     CDS             17746..18906
                     /locus_tag="PKX01_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HMZ76186.1"
                     /translation="MTLPFDFAHGAERAGSDSIKWRRYAGRDVIPMWVADMDFAAPPP
                     VLDALRARLDHGVFGYADPWPGVLEAVIDGIARDHGWSIQPEWLVWLPGVVTGFNVAV
                     RTVGRPGDGVFTATPVYPPFLHAPGNFEQRLVTTPLLRTGARWEWDWDRSAAALADGV
                     RLFMLCHPHNPVGRVFDRDELGRIAELAERHDLVVCSDEIHCGLVLDPTKAHVPLAAL
                     DERIGRRTITLMAPSKTWNIPALYCAFAIIPDGTLRARYKHAMAGIVPHVNVLGLVAT
                     EAAYRDGGPWREALLATLRSNAARVTDAIDLLPGLATTPVEATYLAWIDCRDFLAARG
                     LTGSPAAFFEQAGVGLSDGRDFGLPGFVRLNFGCPPARLEAALERMRRAVTA"
     gene            18906..20156
                     /locus_tag="PKX01_08420"
     CDS             18906..20156
                     /locus_tag="PKX01_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ76187.1"
                     /translation="MGASSASFGLRTAPRDVRLYVVTTVCSALIHFGQGVAVGWSVYE
                     QTGAPLALGFVGLAQFLPIAVLFLPAGHLADRVERRRMVLVSLLLTFAATLLLAYAAW
                     RALPVGWTYAALVLSGCGQILYRPARDALLPALLPPQHLARGIALASALYQVASLAAP
                     AAVGFGLAQVHSAVPIHLCNALLAALALLATIAIRQRSHGQPAATGSRVAEVLAGFRH
                     VWRTRILLAVMSVDLVAVLFGGAVALLPVYARDILHVGPAGLGLLSTAPALGAACAGL
                     VMTRSGVALDGRVLLAAVGGFGVATVVFGLSTAFPLSLAALAVAGGCDCVSVVLRQTV
                     LQQATPDALRGRVGAVNRVFVSSSNELGALESGLAAAWFGPLLAVAGGGAITVLVALG
                     APALFPVLRALRHDRPLPRDPAVS"
     gene            20223..22034
                     /locus_tag="PKX01_08425"
     CDS             20223..22034
                     /locus_tag="PKX01_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HMZ76188.1"
                     /translation="MTPLPSAPADALDGILQRHRHDATRLLQILIDAQDLDGWLPPPM
                     LTRIAAALDLPRARVEGVAGFYSFLHTASVGRYRVLFSDNITDRMLGNAGLMDALCAK
                     LWVEPGKRSEDGLVSIATTSCTGLCDQGPALLVNGRAIGQLDHRRINAIAELIRQQTP
                     LERWPAEFFRVTHNIRRRDALLNLDMAPGAALSAVLALGREGWLTEMEASRLRGRGGA
                     GFTTSVKWLSARNAPGAEKVIVCNADEGEPGTFKDRVLLAGFADLVFEGMTLAAWAVG
                     ARQGLLYLRGEYRWLRPHLDAVLARRRSAGLLGPAILGERGFDFDIDIHLGAGAYVCG
                     EETALIESLEGKRGTPRIRPPFPVTHGYLGRPTVVNNVETLAKTCLIALQGGAAFAAT
                     GTAQSAGTKLLSVSGDCAAPGIYEYPFGVTLARVLEDCGASDTQAVQVGGAAGLCLAG
                     YEFQRRIAYEDVPTAGAFMVFDATRDLFEVARAFVHFFAHESCGFCTPCRVGTALLKG
                     FMDKLADGHGAQADLADIEWLDRLLKNASHCGLGSAAPNPVIDTLQKFRPAYERRLLS
                     LDFEPAFDLDGALAEARRLTARNDPGAHLATHEETRE"
     gene            22031..22729
                     /locus_tag="PKX01_08430"
     CDS             22031..22729
                     /locus_tag="PKX01_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ76189.1"
                     /translation="MSKTFLLDGRPVPFEDGQSILQAARAAGQFIPSLCWHKDFPAHG
                     SCKLCIVKAAGRHVSACATPAREGWEVESNTPEMNNERRALLQMLFVEGNHFCPSCEK
                     SGHCQLQALAYEYEMLTPRFRHFYPERPVDASHPDMLLDFNRCIFCELCVRASRDVDG
                     KSVFALTGRGIAKHLTVNAESGRLADTDFAATDLAARICPVGVILPKGRGFAVPIGAR
                     PYDTGTIRDTAQED"
     gene            22880..23425
                     /locus_tag="PKX01_08435"
     CDS             22880..23425
                     /locus_tag="PKX01_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HMZ76190.1"
                     /translation="MTAPRKIRVATASLAGCFGCHMAFLDIDEHLLELAGQVEFDRSP
                     LTDIKDVGPCDVGLIEGGVCNAENVHVLRAFRRQCKTLVAMGACAINGGLPAQRNHLS
                     VADCLREVYQFRPGLADGRIPDDPELPLPLDKVRPLHEVVRVDYFLPGCPPSGAAVWK
                     FLSDLLAGRTPRLTGALLHYD"
     gene            23435..>23777
                     /locus_tag="PKX01_08440"
     CDS             23435..>23777
                     /locus_tag="PKX01_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMZ76191.1"
                     /translation="MSLELETAADRAHLRRVAIDPVSRVEGHGKVTLLLDEGGHVQEA
                     RLHIVEFRGFEKFIQGRPFWEVPVMVQRLCGICPVSHHLAAAKALDTVVGATITPTAE
                     KIRRLMHYGQIL"
BASE COUNT         3735 a         8658 c         7713 g         3671 t
ORIGIN      
        1 gtggagccgg gacggcaggg gccgccggca cggacggcgg cgcgggccgg ggcgcttcga
       61 cggacttggg ggcgctcgcg cacgccgcca gcagcaggac tgccgaggcg ggtgccagga
      121 ggcgggacag ggaagctcgg tccatggatg gggtacactc gcgatccgga tattcgggct
      181 gcccgagtat ataagctcac tccccaggac gctccatgtg gttgatcggc ctcgaactcg
      241 gcgttgtcgc cgtcctgctc ctgatcgtcg ccctggccct gcgcgcgccc aaggacaaaa
      301 acgacgaccc gcctccccat gactgacctg aacgatctca tcgcccgcgc cgggcagttg
      361 ctcgaccgcc tcgaaggcct gctcccggcc gcgccgactg ctcccgactg gcacgccgcc
      421 gtggccttcc gctggcgccg cagccgcagc gccgccaccc tccagccggt gctgcgcccc
      481 cacgcgatcc gcctcgacga cctgcaggac atcgacgaca agaaggcccg catcgtcgcc
      541 aacacgcgcc agttcctggc cggcaagcgg gccaacaacg tgctgctcac cggcgcccgc
      601 ggcaccggca agtcgtccct ggtgaaggcc gtgctcaacg aattcgccga cgccggcctg
      661 cgcctcatcg aggtggacaa ggacgacctg atcgacctgc cggacatcgt cgaactggtg
      721 cagggccgcc ccgagcgctt catcctgttc accgacgacc tctcctttga ggacggcgag
      781 cccgcgtaca aggccctcaa gagcgtgctc gacggctccg tcgccgcggt gccggacaac
      841 gtgctgatct acgccacctc caaccgccgc cacctgatgc ccgagtactt tgccgaaaac
      901 gcggcgacga agcacaagga cggcgaggtg catcccgccg aagcggtcga ggagaagatc
      961 tccctgtcgg agcgcttcgg cctgtggatc tccttctacc ccttcagcca ggacgagtac
     1021 ctggcggtgg tgaatcactg gctgcgggag ttcggcgtgc ccgccgaggc cgtcgccgac
     1081 gcccgcggcg aagccctgca atgggcgctg ctgcgcggct cccgcagcgg ccgggtggcc
     1141 tggcagttcg cccgcgacta cgccggacgc ttcgacgcat gagcgcctcg cccccgcgca
     1201 agatcgtcca cgtggccgct gcggtcatca cccgcccgga cggctcctac ctcctcggcc
     1261 agcgtgcgcc cggcaccttc taccccggct actgggaatt cccgggcggc aaggtggagg
     1321 ccggcgaaac gccccgcgac gcattggtcc gcgaactcgc cgaggaactg ggcatcaccg
     1381 tggagaccgc gtggccgtgg atcgtgcgcg agcacgtcta cgaacacgcc cacgtgtccc
     1441 tgcacttctt cgaggtccct gcctggcacg gcgagctgac ggacaaggtg cacagcgccc
     1501 tcgcctggca acacccggac gccgttgacg tgacccctgt gctgcccgcc aacggcccca
     1561 tcctcaaggg cctgcgcctg ccccgccgga tgggcatcac ccaggccggc gcagtgggcg
     1621 tcgaacgtca gctgaagcgt gtggacgcgg ccctcgccgc gggcctgcgc ctggtacaga
     1681 tccgcgaacc ggagctggac gccgagatgc tcgcctgctt tgcccgcgag gtcgtggccc
     1741 gttgtcacgc cgtcggcgcc ctcgcggtgt tgaacggcga cgcggaactc gcccacacgc
     1801 tgggcgccga tggtgtccat ctgcccgccc ggtgtctgat gagcctggcg gcccggccgg
     1861 atctggattg ggtcggcgcg tcctgccaca cccgcgccga actggaacgg gcggccgccc
     1921 tcgggctcga ctacgccgtg ctcggcgcag tgcaggccac ggcgacccac ccggagcgga
     1981 agcccctggg ctggagcggc ttcacgacgc tcgccgccgg gctgccgctg ccggtcttcg
     2041 cactgggggg cgtgcagcgg gatgacctgg aaacggcccg cgccgccggt gcccatggca
     2101 tcgcagccat ccgtgctgcc tgggacacct gaaacccggg gacgcggccg cgctgccggg
     2161 cccgcgccgc gtcaggtgcg cgtgagggga tcggcgccgt cgtcgagcgg cgtctcaccc
     2221 gccacccggt aggtgtcgga cgcccacgcc ccgaggtcga tcttgcggca ccgctcggaa
     2281 cagaacggcc ggaaggcgtt ctccggcacc cacaggacgg ccttgccgca ggtcgggcat
     2341 ttgacttcac gggaacggac cggtgtgctc acagattgca gaacgtcagt tcgaagcgca
     2401 cgtccgcctc ggactgacgc ggacggacga cggtttcggc gtgcatgaag cgaatattga
     2461 gggcgtactt gttggcgctg gtttccggca cgtagggatc gccggaactc aggcggatgc
     2521 ggatcatctg cacggtgcgc ccgcccatgg tctgctgata cgctccgcgc gcggccacaa
     2581 gctcttcggg tcggccgctg gcgcgcagca gacgcaacac gatggccagg ccgtcgcgga
     2641 tgggcagcag gggctggacc caggccacca gatcgcgacg acggaactcc acgtcgcggt
     2701 tgagccaatg gtgatacgag ggcaggtcga actcacaggc gccgccggga atcgccgcac
     2761 gactgcggat gttcatcagc cattcgttct cgcgcaggta ctggccggtc ttgccggcca
     2821 tggcgagcag ggatgcagag gcctgctcga tctcgtagag ggcgccggag agggcctctt
     2881 cggagatctg cggattgttg cggaaggaca ggaggatctg gcgctggcgc tccagttcct
     2941 gcaccagatc aaccttcagg tcggcgcggc tggcgacttc gaggatttcg aacagggtga
     3001 cgagggcgac gtggtgctca agggagccgt cggcccgcac gaagtgcatc accttgtcga
     3061 agagatcctc cagacggagg agcgtgcgga tcctctcgtt gagcggatat tcgtaggaga
     3121 tcacgcgcgc gcctgaaaga ttggaggaat tgggcagtct gaccggcacg gatcatatca
     3181 cagcacccga ccccgcgcgg cgcactggat atattcaccg tggagcgccg cgacccgcgc
     3241 ctgcagggcc tccagcgagc cggcgttgtc caccacgtcg tccgcagcgg cgaggcgcgc
     3301 cgcacgcgtg gcctgggccg ccatgatggc gcggacctgg cgttcgtcca gcccgctgcg
     3361 gcacatcacc cgttccacct gcagtgcttc ggggcaatcc accacgcaca gacgctgcac
     3421 gcgctcccga taggtacccg actcgatcag cagcggcacc acgagcacca cgtagggtgc
     3481 gaccgccacg gcacactgac gcgcgctttc gtcgcggatc aggggatgca ggatggcctc
     3541 caggcgcttg cgcacggaag catcctcgaa cgccagggcg cgcatggcag cccggttgag
     3601 ggcgccgtcc gcggcgacga tgccctcacc gaaagcagca cgcagggcgg gcatcgccgc
     3661 accctcgggc gccgtgaggg catgggcaat gcggtcggta tccacaacgg ccgcccccag
     3721 cgtggcgaag cggtcagcca ccgcggactt cccgctgcca atccccccgg tcagcccgac
     3781 aacgaacggc ccactgctca tggcttgcac ccctgacgac tgacaccctt gacgtgatcg
     3841 gacagatcgc tgacgagggt cgccaggctg tcgctgggtc cccgcacctc gagacgcacc
     3901 agggcgctgc cacgggtggc gatctgggca ctgcgcacac ccttggcccg caggacttcg
     3961 agctggcggt tggcagcgac ctcgctcttg aacagcccga gggagatggc gaactgatta
     4021 ggcccggcgt cgggcacgat gtagaggtca tccacgccaa gcttgcgcac ctccgcggcc
     4081 ttgcgatcgg cgccatcctt gctgcccggg gatggaatgt agacccacca ggacgacggc
     4141 tgctcgctgc ggctttcgtt caatttgagc ccgccgccga ccttgccgat gcgtgccgcc
     4201 agttcctggg cctgatccac acccagcccg gcaaaggcca cacaggcggg cggcgcagag
     4261 gtgtccgtgg caggcgcgga ttcgcgctgc gccggcgcgg ccggggtcgg aacaggcggc
     4321 tcaggcgaag tccggggtgc ctcggcgatc gcttcccgcg gccggggcgc tggcggacgg
     4381 gcatccaccg cgagcttgag gcgctccgcg ttcagctggt tgctgagccg ttcaggctgg
     4441 ccatcggaat cgcctccgcc cagccagccc ttccacaagg cgagcgccag caggttggcg
     4501 aagatcagga caatgacggc gacacgcagg ggaaccatgg acacaagcct aacgtaacgg
     4561 tgcggcacgg agaagcatcg gactcaactt gcatcatttg cccgatgtca ggcgggccag
     4621 gccttccagc accagattgt cgacctgacg gacgggacag ccgagctgcg ggcccagatc
     4681 ggccgcggca ccgccgttga gcagacagac cgcgtcaggc gcatcggcca ccagccggaa
     4741 catgcgttcg atggcccctg cctgggcatg caggcagcca ctgacgatcg cgtcggcggt
     4801 acgccgtggc gtcgcgtcga agcgcccgtc ggcgaagggc aggcgggccg tgtcgcgggc
     4861 cagcgcccgc cgcatcaatt ccagcccggg caggatgatc ccgccgcgga acacgccttc
     4921 ggccgtcagc acatccaccg tcgtcgcggt accagccgtc accaccagcg cggcaccggc
     4981 atgcagggtg cgggcaccga tcagggcggc ccagcgatcc gggcccagtt gggtcggatc
     5041 cagataggcg ttgcgtaccc cgccggcttc aatgcccggc accagccatc cgggcgtccg
     5101 cttccacgcg gcacacaggc gccccaccgc gagggcgaca gacgccccgg ccacattggc
     5161 gcccatcacc cgtgcaggca ccggctgaac agccagggcc gcctccagcg cttccggcgt
     5221 ggcggtcgga acggcgcccc gcgcgtgcca gacagcgcca tcatgcagcc cccacttgac
     5281 acgcgtattg cccgcatcga tgagcaggtt catgcgacgc tccgcaggct cacttctccg
     5341 gagagcaccc tttgaagacc ggaacccgta ttcagcagca gggcgccatc ctcgtccacg
     5401 ccgacgcacg tcccttcctg cgagacggtt tcgccgagaa tgcgcaccgg cagaccggcg
     5461 tgggcgttgc gttgctgcca ggccccgcgc aacgccggaa aacccgccgc ggcatacagt
     5521 tcaagcagac cgcggaattg gtcaagcaga cacccgagga gccgcgcccg cgtcggcggc
     5581 gccgtcaggc aggcggccag atcggtgacg ccgcctgcgt gggggatgtc ggcctccggc
     5641 ggcaggcgca ggttgagccc cacgccgatg accgcggcgg gggtccgtcc gcgccccgga
     5701 atgagctcca ccaggatgcc cgcgagcttg cgtccgtgga tcaggacatc gttgggccac
     5761 ttgagctgca ccccttcgac ccccaactct tcgagcgccc gcgccactgc cagtccggcc
     5821 accagggaca gcccggccgg caccggcgcg cccggttcga accgccacag caccgagaag
     5881 gtcagacccg cgcccggcca cgacagccag ttgcgcccac ggcgtccgcg acccgccgtc
     5941 tggcggtccg tgacgaggac atggatgcgt ccgtcgtccg ccggcgggtc atcgaggagg
     6001 acggaattgg tggaggcgca ctcgggcacc acgaccacgg aaaaggccgc ggcgtggtcg
     6061 cccaggacgg cgagcaccgc gtcgcggtcg aggggttcgc aggacaagga atgcaagggt
     6121 cacccggcag gcatgatcga ttcgccatta tcacggattt ccttgccgca acttgatggc
     6181 cggacggacc ggctgtggtc tgccgccgac gggcggcagt ctggcggggc cggatcgact
     6241 caggcgtcgc gacagaattc ggtcgaccga ccatgcggac aagcctcggg accgctgtcc
     6301 gcgggtgacg ggcacccgcg gacagccgcg cccctgccgc tcaggcgtgc tcgccctctt
     6361 ccagtcccag ttcctggatc ttgcgggtga tggtgttgcg gccgataccg agcagctgtg
     6421 ccgcctcgat gcgccggccc ccggtggcgt tgagggcgcg ccggatgagg atgcgctcga
     6481 agtcccgcga caggcggtcg aacacttctc ccggattctg caccagggcc cgatccgctt
     6541 ccgcggtcag ggcgtccacc cagttggccg gctgctcgcg ggtgggctgc tccttcaact
     6601 ccggcggcag gtcggcgact tccaccgtct gccccggcgc catgacggtg agccagtggc
     6661 acaggttttc gagttggcgc acattgcccg ggaaatccag gctctgcagg tacttgaggg
     6721 tggcatccga gaggcgcttg ggctccacgc cgagctcctg cgccgacagg gcgatgaagt
     6781 gcttggccag catcgggatg tcctcgcgcc gctcgcggat cggcggcaga cgcaggcgga
     6841 tcacgttgag ccgatggaac aggtcttccc ggaacaggcc ctgacgcacc cgctcctcca
     6901 tgttttggtg ggtggcggcg atgacgcgca cgttggcctt gatgggctga tgaccgccaa
     6961 cccggtagaa gttgccatcg gacagcaccc gcagcaggcg cgtctggagc tcggccggca
     7021 tgtcgccgat ttcgtcgagg aacagggtgc caccctcggc ctgctcgaag cggccgcggc
     7081 gcatggtctg ggcaccggtg aaggcgccgc gctcgtggcc gaacagctcg gattcgagga
     7141 ggtcgcgggg aatcgccgcg gtgttgatgg cgatgaaggg cgcgtcgcgt cgcgggctgt
     7201 gacgatgcag ggcgcgcgcc acgagctcct tgccggtacc ggactcgccg ttgatgagca
     7261 cggtggcgtg ggactgggac agccgcccga tggcgcggaa gacttcctgc atggacggcg
     7321 cctgaccaag gatctcgggc accgcgctga gaacttcctc accagcgtgc tggtgggcgg
     7381 tctcgtcgat ggcacgacgc accagctcga ccgcctggtc cacatcgaag ggcttgggca
     7441 ggtattcgaa agcgcccccc tggaaggccg ccacagcact gtccagatcg gagtaggcag
     7501 tcatgatgat gaccggcagg tgcgggaagc gtgccttgac cttggacagc agggtcagcc
     7561 ccgactcgcc cggcatgcgg atgtcggaca ccagcacgcg cggcggctgg gcggcactgt
     7621 cgagcgccgt cagcacgtcg ttggcattac cgaaactctt gtaggggatt tcctcccgcg
     7681 ccagggcttt ctccagcacc caacggatgg agcggtcatc atcgactatc cagactgcac
     7741 tcattttctc tcacacgctc agggaggcca ggcctcgacg tcaactgttg gcaccacatt
     7801 ggtgcattct cttcagggga tcacacatcg ctgtcccgcg tgatgggcag gaagatcgtg
     7861 aaacaggtcc ggccaggccg ggagtccacg tcgatcaccc cctgatgctg ctcgatgaag
     7921 ctctgagcca gggacaatcc cagtccgctt ccgttctccc gccccgacac cagcgggtag
     7981 aagatcttgt cgcggatctc ggcgggaatg cccgggccgt tgtcgatcac ttgcaactgt
     8041 aatgccagtt tgaagcggcg cttggccatg gtgatctgac gggccgcgcg ggtacgcagg
     8101 acaatctcgc cgtcgccatt catggcctgc gcggcattgc gggtgatgtt gaggatggcc
     8161 tggatgagct gctcgcggtc accggtcacg tagggcaggc tggtatcgta gtcgcggcgc
     8221 acctcgacgg agggaaactc cgccagcacc agacggcgca cgcgttccag cacgtcatgc
     8281 acgttgagtt gcgacggctg catcatgcgg tgggacgtga gcaggcggtt catcaggtcc
     8341 tgcaggcggt ccgcttcggc aatgatgacc tgggtatatt cgcggagctg cgggtcgtcc
     8401 agttcccgct ccagcagctg ggccgacccc cggatgccgc ccagcggatt cttgatctcg
     8461 tgcgccagat tgcggatcag ctcgcggttt gcctgttgtt ggtgcgcgag ctgctcttcc
     8521 cgtgccactt ttaggtgcgc atccatgggg cggaactcca gcaggaggcg caccgcatcg
     8581 gactcgacgg gcgtgacggt gcaatccagg tgcaaggctt ccattccggg gcgcaggacc
     8641 gtgagattgt ggccggtgta gctccagttg tcccgcagca cgttgttcag ggccgtgccg
     8701 agcccggccg gctcaccgag caggcgacgc agagagtagc cgacgatctt gcggctgctc
     8761 acggcaaaca ggttctcggc ccccggattg agatagcgga cgatcagctc gtcgtccaca
     8821 agcaccacgg ccgacgacag cagctcgagg ccggcaaagg gcgaaggggt ctggtcaggc
     8881 gggtcgtttt gcatagcttg caagcatgca agaaacagac caacaggcac cgcctctggg
     8941 cccttgcggg caccgcccaa ccggctgaag atcagcgcag attggcgatc tcgcggcgca
     9001 gggcttcgat gttgcgctcg tggagctgga ccttgtcctt gaacggctgc aggcggtcga
     9061 gcacgcgctg atagttgcgt tcatccccgt tgcggatcgc ctcctggtcg gccagatcct
     9121 tctgggccac cgccaggctg ttctgttcgg tggcgagctc gtcttcgaga atgcgccgac
     9181 gatcattgtc ccgcgcccgc tgggtgcccg catcgacacg cgggaagccg gccgccccgg
     9241 atgcggacgg cgctgcaggc gccgtcgaag gcgcaccagc aggccgcgac gccggcccgg
     9301 ccggcatcga cgagaccggc tgatcccgcg acagcagact gcaccctttg ccgcccacct
     9361 tggcattggt gtacgtgaca tgcccgccct cgtcgatgca tttgaaaatg tcggcgaggg
     9421 ccggggcggc ggacagcagg gcggcgaggc ccaggacgac gggaagcttc atggtctcga
     9481 acgggcgcgc aggcgcgttg aacacggccc ccctagtgta tgtgccccct gttgcaaaca
     9541 caaacgcggt cctgtaacaa aaaaaggacg gaattgcttc cgtccttttc aggcaccacg
     9601 cgccggccgg aaacccggcc ggcactgatc gcgcaggatc agatggcgta gtacatctcg
     9661 aactcgaccg ggtgcacttc gacgcgcaga cggttcactt cgtcgttctt gagggcgatg
     9721 aaggagtcga tgaagtcatt gctgaacacg ccaccgcggg tcaggaactc gcggtccttg
     9781 tccagggctt ccagagcctg ttcgaggctg gagcagacgg tcgggatgag cgcgtcttct
     9841 tccggcggca ggtcgtacag gttcttgtcg gcggggtcgc ccgggtggat cttgttctgg
     9901 atgccgtcca ggccagccat catcagcgca gcgaagcaca ggtaggggtt cgcgcccgga
     9961 tccgggaaac gggtctcgat gcggcgggcc ttttcgctgt tgacgaacgg gatgcggatc
    10021 gaagcggaac ggttcttggc ggagtaggcc agcttcaccg gggcttcgaa gtggggcacc
    10081 agacgcttgt aggagttggt cagcgggttg gtgatcgcgt tcagggcctt ggcgtgcttg
    10141 atgatgccgc cgatgtagta cagggcggtc tcggacaggc cggcgtagcc gttgcccgcg
    10201 aacaggttct tgccgtcctt ccagatggac tggtgcacgt gcatgccgga accgttgtca
    10261 ccgacgatgg gcttgggcat gaaggtggcg gtcttgccgt actggtgcgc cacgttctgg
    10321 acgatgtact tcaggatctg ggtctggtcg gcacgacgca ccagggtgtt gaacttggtg
    10381 ccgatttcgt tctggccggc gttcgccact tcgtggtgat gcacttcgac ggggacgccg
    10441 caggcttcca gagccagaca catggcggcg cggatgtcgt tgaaggagtc aaccggaggc
    10501 acggggaagt agccgccctt gaccgcggga cggtagccgg tgttgccgcc ttcgtacttg
    10561 atgtcggacg accaggcacc ttcttcggag aagatcttgt ggtaggcgcc ggacatgtcc
    10621 accttccact cgacggagtc gaagatgaag aattcgggtt ccggaccgaa gaacgcggtg
    10681 tcaccaatgc cggaagcctt cagataggct tcagcgcggt tggcgatgga gcgcgggtcg
    10741 cggtcatagc ccttgccgtc ggcgggatcg atcaggtcgc aggtcaggac cagggtggtt
    10801 tcgtcgaaga acgggtcgat gtaggcggtg ttggggtccg gcatcagcag catgtcggag
    10861 gcctggatcc ccttccagcc ggcgatggag gaaccgtcaa aggcatggcc gctttcgaac
    10921 ttgtcttcgt cgaaggcgct gaggggcacg ccgacgtgct gttctttgcc cttggtgtcg
    10981 gtgaaacgga agtcgacgaa cttgacttcg ttttcctgga ccatcttcat gacgtcttgc
    11041 ggggtcatat aactcactcc taacgggttg aagctgctgc gtagtggata aaggaacaaa
    11101 accaggaatt cgggtatcgc tcccggtagc accaagcgtg ccaccgccgg gaagggatca
    11161 cgctattgtg ccataagggt taactgctgc cagccggaaa ttgacccgca ccactcaagg
    11221 gccccttcaa cacaaacgca ccactatggt gcaatctgac acagcagcgc gatgcgcccc
    11281 cagtgaggcc ggcgggcaac gccgtccgcc acctgctgcc gcagggcgtc gatgcggcgc
    11341 gcatcgcaac accagacatg gggcagaaag acgtccgcct ccacctgctt gccgacatag
    11401 tgcaggatga tccgctccgt ctgcgggagg tccggcccga ggatctcggc gatctccgcc
    11461 aggagcacgt cccgggacgg cagatggcgg ccggcggcga gttcctcgtc ctcttccgta
    11521 tccacatgga ccagcacgtc gagcacgtcc tccacccggc tgcgcacgcg gtgacgggcc
    11581 agctcggcga tgcgatggcc ttccgacaca ctgatacgcg catccacccg cacgtgggcg
    11641 tccaccagga tgcgatgggc catgcgccgc gtgcgcaggt cgtgcacccc cagcacgccg
    11701 ggcgtttctt ccaccgcacg gcggatggac gccacctctt ccggcgccac gccggtgtcg
    11761 atcagctcgc gcaaggcgtc gaaggacagt tgcagcccca tgcggatgat catgaaaccg
    11821 accagtgccg cggcgagggg ctccagaaag cggtagccca gcaggctgcc cccgatgccg
    11881 accgccacca ccagcgacga cgctgcgtcg gcgcgggcgt gccaggcatt ggcgaccagc
    11941 atcggcgagc gaaggcgctc ggccaccgcc agcatgtagc ggaacaggcc ctccttgcca
    12001 gccagagtca gcagcgccat ccacagggcc gcgggatgca gcgcgggcaa ggcttcggtg
    12061 ctctgcagac ggctggcggc ggagagcagg aagccggctc ccacgccggc cagcaccagc
    12121 ccgaggatca tcgacgccgc cgtctcgtag cggccgtggc cgtagggatg gcgctcgtcc
    12181 gccggcgtgg agccgcgccg gctcgccacc atcaccatga aatcgccgag caggtccgac
    12241 agggtgtgca taccgtccgc caccagactc tgggcatggg cgaacagccc cacggcgatc
    12301 tgcagcaccg tcaggacaac attgacggcg atgctcaccc aggtggcctg ctgggcctga
    12361 cgggcgcggt cggcatcgag gggcgcctcg gtggcggccg agagagggga atcgtgggcc
    12421 atgacttgca gcggaagggc gcgggggtat actcgcaggc ccgccattct agccggatgt
    12481 ccgccatggg aaaattaagc gagaccctcg cactggcgca gcaacgcagc cgcgacgccg
    12541 gcctgcccta cgccggcctg ctcacccccc gcgaagcctg ggacctgctc caggccgccc
    12601 ctggcgccag gctggtggat gtacgcaccc gcgccgaatg ggactgggtc gggcgcgtgc
    12661 ccggcgcggt cgagcttgaa tggatgctct accccggcaa cgagcccaac ccccacttcc
    12721 tcgcccagtt gcagcgccag gtcgatccgg aagccctggt gatgttcctg tgccgcagcg
    12781 gggtccgttc caacaacgcg gccaccctcg cggccggcca cggttacacc aacgcctaca
    12841 acatcctgga gggcttcgag ggcgacaagg acgcccacgg ccatcgcggc aagctgggag
    12901 gctggcgctt cgccggtctg ccgtggatcc agggctgaca ccgactcaca ggattgaccc
    12961 gacgatgcag accgcctcca tcagcttcga ccgcttcaac gccctggccg acgccgacgc
    13021 ccacgcgcgc atccgcgccg cccgtgcccg cctcggcgac aaggccgtgc tgctgtgcca
    13081 ccactaccag cgcgccgacg tctaccagta cgccgacatc accggcgact ccctcaagct
    13141 ttcccgcctc gcttcggaca ccgacgccga gtacatcgtc ttctgcggcg tgcacttcat
    13201 ggccgaagtg gcggacatca tgtccaggcc gcagcagatc gccatcctgc ccgatctggc
    13261 ggccggctgc tccatggccg acatggccaa tctggccaag gtagaacgct gctggcgcga
    13321 actgacggaa gtgctcgaca cgccggatgc cctgatcacc ccggtcacct acatcaactc
    13381 cgccgcggat ctcaaggctt tctgcggcga gcacggtggc atcgtgtgca cctccaccaa
    13441 cgccccggtg atcctcgact gggccttcgc ccagcgggag aaggtgctgt tcttcccgga
    13501 ccagcacctg ggccgctgga ccggcttcaa caaaggcatt ccgctggacg aaatggtcgt
    13561 gtgggatccc gacctggaat acggcggcct caccgccgaa cagatccgca aggccaggat
    13621 cctgctgtgg aagggccact gctcggtgca ccagatgttc cagcccgccc acatcctcaa
    13681 gttccgcaat cagcatccgg acggcaaggt catctcccac ccggagagcg ccctggaggt
    13741 gtgccgccag tccgacttcg tcggctccac cgagtacatc atcaaggtcg tcaaggaatc
    13801 tgcccccggc acccgctggc tggtcggcac cgagctcaac ctggtcaacc gcctcgccga
    13861 agaagtgaag cacgagggca agatcgtcca gttcatggcg cccaccgtgt gcatgtgctc
    13921 gaccatgcag cgcatcgacc cgcagcacct ggcgtggacc ctggagaacc tcgccgaggg
    13981 caacgtggtc aaccgcatca ccgtgcccgc ccacgaggcg gccctggcca aggtggcgct
    14041 ggaacggatg ctcgccgtgt cctgatcctg cgccagcacc tcaagacggc gcccgcgggc
    14101 gccgtttttc ctggcggcca tccctgcgga cgaagaagga acggacatga gcaccctgcg
    14161 catctgcacc tacaacattc acaagggctt ctcccagttc aaccgccgcc tcgccatcca
    14221 cgacgtgcgc gagcgcctgc accagctcgg cgcggacgtg gtcttcctgc aggaggtcca
    14281 gggccagcac ctccaccacg cccgccgcca caccgactgg cccgaacaac cccagcacga
    14341 attcctcgcc ggcgacctgt ggcgcgacac cgcgtacggc ggcaatgccc tctacgacca
    14401 cggccaccac ggtaacgcga tcctcagccg ccatcccatc ctgtcccgcg ccaaccagga
    14461 cgtctccgac caccgtttcg aacggcgcgg tctgctccat tgcgagatcc ggctctccgg
    14521 cgtggcgcgc ccggtgcatt gcgtctgtgt gcatctgggg ctcaccgccg gcacgcgcag
    14581 gcgccagatg gcggcgctgg tggcgcgcct caacgtcctc gccccgccca ccgagcccct
    14641 catcatcgct ggcgacttca acgactggcg caaccacgcc gacgaccacc tgatccgcca
    14701 gctcggcgtc gtcgaggtgt tcaccgccct gcgcggccga ccggcgcgca gcttcccgtg
    14761 ccacctgccg atgctgcgcc tggaccgcat ctacgtgcgg ggcttcgcca tcgaccaggc
    14821 ggaggtgcat ttcggagcac cgtggaaccg cctgtccgac cacgcccccc tcaccgccca
    14881 gctgcgcgcc cactgagctg ccgccatggc catccactgg ctgcctggca acgccctcac
    14941 cctgctggag aacggcgcgg aattcttccc cgccctgcag gccgccatcg acactgcctg
    15001 ccacgaggtc tatctcgaaa cctacatctt tgcggatgac gttaccggtc gcgccatcgc
    15061 cgccgccctg gcccgcgcgg cggcgcgggg cgtcaccgtg cgcctgctgg tggacggctt
    15121 cggcggacgc gaattcgtcg agcggctgcg ggacgacctg atcgcccgcg gcgtccatgt
    15181 cctcatctac cgccgcgaca tcagtccctt cgccatccgc cgtaggcgcc tgcgccgcct
    15241 ccaccgcaag ctggcggtca tcgatggccg cgtcgccttc gtcggtggca tcaacatcct
    15301 cagcgacctg tcggagccca acatggcggc cccgcgccac gactacgcgg tgcgcgtcga
    15361 aggccccctc ctcgccccca tccacgccac cgtgcagcac ctgtgggaac tggtcgcgtg
    15421 ggcctccttc cgccaccgtc accgccttcc ggccggcccg gcgccctgct gcggcacctg
    15481 tggcgacgtg gaggccgcct tcctgatccg cgacaacctg cgccaccgcc gcgacatcga
    15541 ggacgcctat ctgctgggca tcgccgcggc acgccgcgag atcgtcctcg ccaacgccta
    15601 cttcctgccg gggcggcgct tccgccaggc cctgctggac gccgcccgcc gcggcgtgcg
    15661 catcaccctg ctgatccagg gcctcgccga ccacccgctc cagcagcgcg ccacccgcgc
    15721 cctctacggc cagctcctgg ccggcgggct gcgcatcgtc gagtaccacc gcagctacct
    15781 gcacgccaag gttgccgtgg tggacgagcg ctgggccacg gtgggctcat cgaacatcga
    15841 ccccttcagc ctgctcctcg cccgtgaggc caacgtggtg atccgcgacg ccgccttcgc
    15901 cacccacctg cgcgaccatc tccaggacgc catcgcccac ggcggccgcg agatctctgc
    15961 cgacgcgtgg cagcggggcc cacgcctgca acgcctgctc aactgggccg cctacggcct
    16021 cgtgcgactg atgatcggcc tcgccggcta cggcggccgg cactgagacc gtcgcccggc
    16081 cggaacatgg gacaatggac gcctcctccg tcccgccgcc agtctccatg aactaccccg
    16141 cccccgcctt cgaagccaag atctgccacc cgcaggacct ggccgcccgc gtcgccgcgc
    16201 tgccccgtcc gctggtgttc accaacggct gcttcgacat cctgcaccgg ggccacgtca
    16261 cctatctggc ccaggcccgc gccctgggcg ccgcgatggt ggtggccctc aacagcgacg
    16321 cctcggtgcg ccgccagggc aaaggcgacg atcgccccat caacgtcctc gaagaccgcc
    16381 tcgcggtgat ggccgccctg gactgcgtgg acctggtcac ctggttcgac gaggacacgc
    16441 ccatcgcccg catcctcgac tgccagcccg acgtgctggt gaagggcggc gactggcccg
    16501 tcgaacgcat cgtcggcgcc cccgaaacct tcgcccgcgg cggccacgtg cactccatcc
    16561 cctttgaaca cgagcgctcc accaccgccc tgctgacgaa gatccgcagg ctgtgacatc
    16621 ggggcgcggg gcactttgtt tattccaatt tgaactattg attcacgtca accagcacta
    16681 gaattaatta gcccccccct caccccgccc gcaaggagcc gccaagatgc gcatcgccag
    16741 caatgtggcc gaactgatcg gcaagacccc cctcgtccgc ctcaatgcac tcgccccggc
    16801 caacggcgcc acgctggccc tcaagctgga gttcttcaac ccggcccaca gcgtcaagga
    16861 ccgcatcgca gccgccatgc tcgacgccgc cgaagccgcc ggcaagatca agcccgacac
    16921 catcatcctc gaacccacct cgggcaacac cggcatcggc ctcgccatgg tctgcgccgc
    16981 ccgcggctac aagtgcgcct tcgtgatgcc cgagacgatg agccgggagc gcaagctcat
    17041 cctcaaggcc tacggcgccg agctgatcct cacccccggc gccgaaggca tgcccggcgc
    17101 cgtcaagcgc gccaccgagc tggccgccgc cgacccgcgc tacttcatcc cgcagcagtt
    17161 cgaaaacccc gccaacccgg ccatccaccg agccaccacg gccgaggaga tctgggccga
    17221 cacggacggc caggtggaca tcttcgtgtc gggcatcggc accggcggca ccatcaccgg
    17281 cgtcggcgaa gtgctcaagg aacgccgccc cggcgtgaag gtcatcgccg tcgaaccggc
    17341 cgccagcccg gtgctgtccg gcggcgcccg cgggccgcac ccgatccagg gcatcggcgc
    17401 cggcttcgtg cccgccatcc tcaacaccgc ggtctacgac gagatcgtcc aggtgcccaa
    17461 tgatgccgcc ctcgacactg cgcgccgcct ggcgagccag gaaggactgc tggtagggat
    17521 ctcctccggc gcggcggtgt gggccgccct tcaggtggcc aaccggcccg agaacgccgg
    17581 caagctggtc gtcgtggtga ttccgtcctt cggcgaacgc tacctgtcca ccgtgctcta
    17641 ccagcacctg gaagtctgaa tcaccccctc cgcaggacgc gcccccttgt ggtcgggcgc
    17701 gtcccgttgc aagctcacgg ccagacacca ttccaggcct ccgccgtgac cctccccttc
    17761 gacttcgccc acggcgccga acgcgccggc tccgacagca tcaagtggcg ccgctacgcc
    17821 ggccgcgacg tcattcccat gtgggtggcc gacatggatt tcgccgcgcc gccgccggtc
    17881 ctcgacgccc tgcgcgcgcg cctcgaccac ggcgtgttcg gctacgccga tccctggccc
    17941 ggcgtactgg aagccgtcat cgacggcatc gcccgcgacc acggctggtc catccagccc
    18001 gaatggctgg tgtggctgcc cggcgtcgtc accggcttca acgtggccgt gcgcaccgtc
    18061 gggcggcccg gcgacggcgt cttcaccgcc acgccggtgt atccgccctt cctccacgcg
    18121 ccgggcaatt tcgagcagcg cctcgtcacc acgccactcc tgcgcacagg cgcgcgctgg
    18181 gaatgggact gggaccgcag cgcggccgcc ctcgccgacg gcgtgcgcct cttcatgctg
    18241 tgccatcccc acaacccggt cggccgggtc ttcgaccggg acgagctcgg ccgcatcgcc
    18301 gaactggccg aacgccacga cctggtggtg tgttccgacg agatccactg cggcctggtc
    18361 ctcgatccga ccaaggctca cgtgcccctt gccgccctcg acgagcgcat cggccgacgc
    18421 accatcacgc tgatggcgcc atccaagacc tggaacattc cggccctcta ctgcgccttc
    18481 gccatcattc ccgacggcac cctgcgggcc cgctacaagc acgccatggc cggcatcgtg
    18541 ccccacgtga atgtgctggg actggtcgcc accgaagccg cctaccggga cggcggcccc
    18601 tggcgcgaag ccctcctcgc caccctgcgg agcaacgccg cccgcgtcac ggacgccatc
    18661 gacctgctcc ccggcctcgc cacgacgccc gtcgaagcca cctacctggc gtggatcgac
    18721 tgccgcgact tcctcgccgc ccgcggcctc acgggcagcc ccgcggcctt cttcgagcag
    18781 gccggcgtgg gcctgtccga cggacgcgac ttcggcctgc cgggcttcgt gcgcctcaac
    18841 ttcggctgcc cgccggcgcg cctggaggcc gccctggagc gcatgcgccg cgccgtcacg
    18901 gcctgatggg cgcgtcatcg gccagcttcg gcctgcgcac ggcgccgcgg gacgtccgcc
    18961 tctacgtcgt caccacggtg tgcagcgccc tgatccactt cggccagggc gtcgcggtgg
    19021 gctggtcggt gtatgagcag acgggcgcgc ccctggccct cggcttcgtc ggcctcgccc
    19081 agttcctgcc catcgccgtg ctcttcctgc cggccgggca tctggcggac cgggtcgaac
    19141 ggcgccgcat ggtcctcgtc agcctgctcc tcacgttcgc ggccaccctg ctgctggcgt
    19201 acgccgcatg gcgcgccctg ccggtgggct ggacctacgc ggcgctggtc ctctccggct
    19261 gcggccagat cctctaccga ccggcacggg acgccctgct gccggccctg ctgccgccgc
    19321 agcatctggc ccgcggcatc gccctggcct cggccctgta ccaggtcgcc tccctcgccg
    19381 caccggcggc ggtgggtttc ggcctcgccc aggtccattc cgccgtgccc atccacctgt
    19441 gcaacgccct gctggcggcg ctggccctgc tcgctaccat cgccatccgc cagcgctccc
    19501 acggccagcc cgccgccacc ggcagccgcg tcgcggaggt gctggcgggc ttccgccacg
    19561 tgtggcgcac ccgcatcctg ctggccgtga tgtcggtgga tctggtcgcc gtgctcttcg
    19621 gtggcgccgt cgccctgctg ccggtctacg cccgcgacat cctccacgtg gggccggccg
    19681 ggctgggcct cctcagcacc gcgccagcgc tcggagccgc ctgcgccggg ctggtcatga
    19741 cccgcagcgg cgtggccctg gacgggcgcg tgctgctcgc cgcggtggga ggcttcggcg
    19801 tggccaccgt ggtgttcgga ctctctacgg cctttcccct gtcccttgca gccctggcgg
    19861 tggcgggtgg ttgcgactgc gtcagcgtgg tgctgcgcca gaccgtcctc cagcaggcca
    19921 cgccagatgc gctgcgcggg cgggttggcg cagtgaaccg ggtcttcgtg agctcgtcga
    19981 acgagctcgg cgccctcgaa tcgggcctcg cggcggcctg gttcggaccg ctcctggcgg
    20041 tcgccggcgg cggtgcgatc accgtgctgg tcgccctcgg cgcacccgcg ctcttccccg
    20101 tgctgcgcgc cctgcggcac gaccggcccc tgccgcgcga ccccgccgtt tcctgatcca
    20161 gcgcaagatt ccgtcatggc ggcggcgcat actgggtaac gtcgacgccc caggagacgc
    20221 gcgtgacccc tcttccgtcc gccccggccg atgccctcga cggcatcctt cagcgccatc
    20281 gtcacgacgc cacccgcctg ctccagatcc tcatcgacgc ccaggatctc gacggctggc
    20341 tgccacctcc catgcttacc cgcatcgcag ccgctctgga cctgccacgc gcccgggtgg
    20401 aaggggtcgc cggcttctac agcttcctcc acaccgcgtc cgtcgggcgc taccgggtgc
    20461 tgttctccga caacatcacc gaccgcatgc tgggcaacgc cgggctgatg gacgccctct
    20521 gcgccaagct gtgggtggag ccgggcaagc gctcggaaga cggcctcgtg agcatcgcca
    20581 ccacctcctg caccggcctg tgcgaccagg ggccggcctt gctggtgaat ggccgcgcca
    20641 tcggccagct cgaccaccgc cgcatcaacg ccatcgccga actgatccgc cagcagacgc
    20701 cccttgaacg ctggccggcg gagttcttcc gggttaccca caacatccgc cgccgcgatg
    20761 ccctgctcaa tctggacatg gcgcccggcg cggccctcag cgcggtcctc gccctcggcc
    20821 gcgaaggttg gctcaccgaa atggaggcct cccgcctgcg cggccgcggc ggcgccggct
    20881 tcaccacttc ggtcaagtgg ttgagcgccc gcaacgcgcc gggcgccgag aaggtcatcg
    20941 tctgcaacgc cgacgaaggc gagcccggta ccttcaagga ccgggtgctg ctggccggct
    21001 tcgccgacct ggtgttcgaa ggcatgaccc ttgcagcctg ggccgtgggt gcacgccagg
    21061 gcctgctcta cctgcgcggc gaataccgct ggctgcgccc ccatctggac gcggtgctgg
    21121 cccggcgtcg ctccgccggc ctcctgggcc cggccatcct cggtgaacgg ggcttcgatt
    21181 tcgacatcga catccacctg ggcgccggcg cctacgtgtg cggcgaggag acggccctca
    21241 tcgaatccct cgaaggcaag cgcggcacac cccgcatccg cccgcccttc ccggtcaccc
    21301 acggctacct gggccgtccc acggtggtga acaacgtgga gaccctggcc aaaacctgcc
    21361 tcatcgccct gcagggcggc gctgccttcg cggccaccgg caccgcccag tcggccggca
    21421 ccaagctgct gtcggtgtcg ggcgactgcg ccgcgccggg catctacgaa taccccttcg
    21481 gcgtcaccct ggcccgcgtg ctggaggact gcggcgccag cgacacccag gcggtgcagg
    21541 tgggcggcgc ggcaggcctg tgcctggcgg gctacgagtt ccagcgccgc atcgcctacg
    21601 aggacgtccc caccgcgggc gccttcatgg tcttcgacgc gacccgcgat ctcttcgaag
    21661 tggcccgcgc cttcgttcac ttcttcgccc acgagagctg cggcttctgc accccgtgcc
    21721 gggtcggtac ggcgctcctc aagggcttca tggacaagct cgccgacggc cacggcgccc
    21781 aggccgacct ggccgacatc gaatggctcg accgcctgct caagaacgcc agccactgcg
    21841 gcctcggcag cgccgcgccg aacccggtca tcgacaccct gcagaagttc cgcccggcct
    21901 atgagcggcg cctgctgtcc ctggacttcg aacccgcctt cgacctggac ggcgccctcg
    21961 ctgaagcccg ccgcctcacc gcgcgcaacg atcccggcgc ccacctggcc acccacgagg
    22021 agacacggga atgagcaaga ccttcctgct ggacggccgc ccggtgccct tcgaggacgg
    22081 ccagagcatc ctccaggccg cccgcgcggc gggacagttc atccccagcc tgtgctggca
    22141 caaggacttc ccggcccacg gcagctgcaa gctgtgcatc gtcaaggccg ccggacgtca
    22201 cgtctcggcc tgcgccacgc cagcccggga gggctgggaa gtcgagagca acacgccgga
    22261 gatgaacaac gagcgccgcg ccctgctgca gatgctgttc gtggagggca accacttctg
    22321 cccgtcctgc gagaagagcg gtcactgcca gcttcaggcc ctggcctacg aatacgagat
    22381 gctcacgccg cgcttccgtc acttctaccc ggagcggccg gtggatgcat cccacccgga
    22441 catgctgctg gacttcaacc gctgcatctt ctgcgaactg tgcgtgcgcg ccagccgcga
    22501 cgtggacggc aagtcggtgt ttgccctcac gggccgagga attgccaagc acctcaccgt
    22561 caatgccgaa tcgggccgcc tcgccgacac cgacttcgca gcgaccgacc tggctgcgcg
    22621 gatctgcccg gtgggcgtga tcctgcccaa gggccgcggc ttcgccgtgc ccatcggcgc
    22681 gcggccctac gacaccggca cgatccgcga cacggcgcag gaggattagc atgaaccggc
    22741 tccgtccctc actccgtccg ccacctgcgc tccggtcgcc gcgcctcggc gcagccgcac
    22801 gggcgccagg ccccgatgct tcaccccacc ccacgcgcag ccgcagcgcg ctcacgtctc
    22861 tcctgcccgg aggcccgaca tgaccgcccc ccgcaagatc cgcgtcgcca cggcgtccct
    22921 cgccggctgc ttcggctgcc acatggcctt tctggacatc gacgagcacc tgctggagct
    22981 ggccgggcag gtggagttcg accgctcgcc cctcaccgac atcaaggacg tgggcccctg
    23041 cgacgtgggc ctcatcgaag gcggcgtgtg caatgcggaa aacgtacacg tgctgcgcgc
    23101 cttccggcgc cagtgcaaga ccctggtcgc gatgggcgcc tgcgccatca acggcggcct
    23161 gccggcccag cgcaaccacc tgtcggtggc ggactgcctg cgcgaggtgt accagttccg
    23221 gcccggcctc gccgatgggc gcatcccgga cgacccggag ctgcccctgc ccctcgacaa
    23281 ggtacgcccc ctccacgagg tggtgcgggt ggattacttc ctgcccggct gcccgccctc
    23341 cggcgcggcg gtgtggaagt ttctctccga tctgctggcg ggccgcaccc cgcgtctcac
    23401 cggcgccctg ctccactatg actgaggtcc ggccatgagc ctcgaactgg aaaccgccgc
    23461 agaccgggca cacctgcgcc gcgtcgccat cgacccggta tcccgcgtgg aaggccacgg
    23521 caaggtcacc ctcctgctgg acgagggcgg ccacgtgcag gaggcccgcc tgcacatcgt
    23581 ggaattccgc ggcttcgaaa aattcatcca gggccggccc ttctgggaag tgccggtgat
    23641 ggtgcagcgc ctgtgcggca tctgcccggt gagccatcat ctggccgccg ccaaggccct
    23701 cgacaccgtg gtgggcgcga cgatcacgcc caccgccgag aagatccgcc gcctgatgca
    23761 ctacggccag atcctcc
//
LOCUS       DAOQPA010000047        23697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1015_length_23697_cov_18.970096, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000047 DAOQPA010000000
VERSION     DAOQPA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_23697_cov_18.970096"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKX01_08445"
     CDS             <1..383
                     /locus_tag="PKX01_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ76192.1"
                     /translation="RVLGMTQEQFAQATGIHLSAIRKYESRSSLPSGESLLAIAGTGI
                     NLHWLLTNEGGMRAPPEAGAQPTQVSPTAELAQRLTAIEGLLGGLDMDKRQAVLNEIF
                     SRVQEAKRVADLEEVVRGLERKVG"
     gene            complement(408..1265)
                     /locus_tag="PKX01_08450"
     CDS             complement(408..1265)
                     /locus_tag="PKX01_08450"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HMZ76193.1"
                     /translation="MIQQSHLTAWQSHAPWPRRSQIEQDLRLSRGVAAIFADPLLGEY
                     LAMRGGTVLHKAHLAPAARYSEDIDLVLVKTMDADTLDRHLRRVLAPVLGQPADSLIA
                     DAWLSIRNVLRPSRILRTAYRFVPLGLRREETIKVEVNLNESASLYPLVGVELDTLDD
                     DGDVVRATARSYDINEMLGTKTRALMQREQGRDLFDLFHAWQLSEAGATPYAVDGAKA
                     MEAFAWYLEKEGAHMDADEANTQLAERLRKRAFRRDMDTLLRPSLPRFDVDAAAAVVR
                     EAYFKHLKR"
     gene            complement(1262..2077)
                     /locus_tag="PKX01_08455"
     CDS             complement(1262..2077)
                     /locus_tag="PKX01_08455"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HMZ76194.1"
                     /translation="MSASSPLSAVLDAAERSGTLNLRTEDIRAALPSVGAQALRQALL
                     RQQRRGRLIRLSRGSDHWLIVPLQYAAAGAPPMEIWLDRYLGKTLGIPYYVALLSAAE
                     IHGASPYAVMVTQVMVAERRRPITVGRHRVVFHTCARIERMPTRWHETPDGRFKVATP
                     ELTALDLVRREALVGGMARVREVLHGLWNACTPQGLSQALEALQDVPTAQRLGALLNL
                     DGQDALASSVADWLRGRLLRVVSLEDGRAPGTSAVIDALFKVRFAPDQGSASA"
     gene            2295..2606
                     /locus_tag="PKX01_08460"
     CDS             2295..2606
                     /locus_tag="PKX01_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ76195.1"
                     /translation="MTAETLFQSLRRMPEMERERFFGLLCASAFRGEDLSHEELFWHL
                     AGDEFTAKEAAEYLGVSMSTFRRFVASGRFHPSSTVGRNQMFAVPELKAFKKALKMAR
                     G"
     gene            2646..3197
                     /locus_tag="PKX01_08465"
     CDS             2646..3197
                     /locus_tag="PKX01_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76196.1"
                     /translation="MTTEQLLFWLAVFGNLILLYHTIKIGFLTVTLLRKGDRAGALWN
                     ASAASALVGLTALFPWTLVAGEREIGKVALLICILTPFVVSMTKLWPSVKGRIAQAPM
                     LATDAAIVGMVVAFAVSGNWVWFGIAGMAMAVIWIVARKSAPRKLQMAVSQEVQFEPA
                     NDPFEEFYGPERDIANPYHIGHR"
     gene            complement(3201..6443)
                     /locus_tag="PKX01_08470"
     CDS             complement(3201..6443)
                     /locus_tag="PKX01_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HMZ76197.1"
                     /translation="MNFEVYAYWNTVELAAVFNAIAAITGSGDFNGLLRTLALVAILS
                     LAIGVLAGRGRHDDFWRWVFMLAIFHGMLLVPKSNVVIVDRTGTAPAQTVANVPIGLA
                     SLAHGTSKIGDWLTTAFETVFSLPDDVQLRKTGTLFGHRVMREKLAAGTVNPLLLHNL
                     MEFYRECIWPDMASGYISSRDISEAPDIWALFNNTNPGLLVTILDYNDPATSNTVSCP
                     GAYSTLTSQLALDVDQQLGAVGSRLYPGMSASAAKAAFTGALATTTNYLLGVSQSAQG
                     ILRQAIVGNTIIDAEYTIPSQLGDAAQAQVKLAQAQAIRSFNTSYGTMTALADETMPK
                     LRNAVEILTYALFPIVVLIIVISGQFAVTFLKAYLGSLLWVQLWPPLYAVTNFIMNTK
                     AATGLSGATEGNGLALKYYNYLGAAVASDQAVAGVLAVAIPVIAYGIVQATMAAANSV
                     VSSVTAGGAYGASASQGNLSMGNATLDTAKTGLHTAGQYNTAPTMRSGMPVATLVGAG
                     ASTTTFADGTTAIDASGMQHRMNVRLNAGSRMSAALQQQSEQTETAAIGNMVSAAATT
                     AAALQQSADFVRTHGKGERAGTHFGLGDQAGFTQAVNQAQKVVDSFAKKHGLDQSQSA
                     QLLGMAEVAMHNPKALDLVSPVSVRAAAKVAGTSETTAKQLLEEARNFAKETGFSEAV
                     DKVRRASREATFDTSDESGRRAMAGIRSSLDQSQQHLDQASTSHQQSLAYKEAASRAR
                     ENSGAWETGLLRQFTDWMGTQYNTFAGRNFDAVTVAQMAEKNPELMTPFVERFFNERV
                     EPALSSGVGEVKSAGDVQAFFEHGKAGVPTANDVARHGQGWLGHVKGAAAGAGVDPSK
                     VVTSGLPQQVAAEQGRAQHALNAGKGGVESQGRPLEQKIHDQTAPGTQPLLGIAATNA
                     VGQVMPDGVSKAMMEKLPGVDMSVGTPGSAVAEAAAERSREGHPRDPVRQSLVGVNDA
                     IERGVSATLPGSVNHALEQTRMHGEQKIGEVIGTRQAQESAPSPLSTGVGEVKTAEEG
                     GDVLGRTLALGESKIGELIGTRPATATQPAEEDGGVKRPGGSEPPPPSSR"
     gene            complement(6456..7895)
                     /locus_tag="PKX01_08475"
     CDS             complement(6456..7895)
                     /locus_tag="PKX01_08475"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HMZ76198.1"
                     /translation="MPGKRLLAAVLIVAFSASATASVQSEMQTWFNEMGAYGNVTGAQ
                     VVQGQTTTVYTGGSMYMRTPIRNYQLASIAPPSIRAGCGGIDLFAGSFSFINSEQLTA
                     LLRNIANNAVGYAFMMAVKSISPDLADLLQYLQDQSSKINGMNLNSCQMAEGITTATL
                     GPLLSDKKEQANAQGTGSLLSNLWPDSFQSFDQWRQDKNAKKQARQQAANADANLKEV
                     LQPGNVVWKALRKTDTPDELKELMMSMVGTIIIVPSGENYGSGMDNSNGEKPLWVYKG
                     PTGIKFSDFIGNPESATKNDITLLKCGGDPDCLNPTVVSNQSVASLSWHVRQTMEKAT
                     SNIMSRQSQSFDGIDYAVYMNSSIPVWRLANIAAMSRGGPALLTNNYSQTVAVELAYN
                     WFTEMVRTLQKALANNSAAQSQDIVQAAASLNEQIANARKLAAAEFTAQYQKAVSMAE
                     LQKTTQWLHETMMRSMPVDVQKSLLTFNR"
     gene            complement(7909..8700)
                     /locus_tag="PKX01_08480"
     CDS             complement(7909..8700)
                     /locus_tag="PKX01_08480"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HMZ76199.1"
                     /translation="MLAVAAAGSGSALATAASGGAGIDYPSIWACDRSKFNWYCDLEP
                     EPPGPATEPDEPKKAKSREEQALERIETLQKELKAKRALAIVDPTPDNLKAYIAAQEA
                     MMNMGSVFSDVWRRVVWQNPDLNYELKRPVNNAAIATYASARKAAEKRQLSDINKEWG
                     LFFFFRSDCPYCHRLAPTLRFLTDTYGITVLPVSVDGAGLPEYPNPQRDNGLVEKLGI
                     RQVPMLVLGNVKDKRLMPLASGVISAQDVIERIYILTGTQPGELY"
     gene            complement(8724..9089)
                     /locus_tag="PKX01_08485"
     CDS             complement(8724..9089)
                     /locus_tag="PKX01_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76200.1"
                     /translation="MKAHVITWAVAVLSACIVGAAWVHTHPWPLMVRVDMGSLFEEQK
                     KALADRIKPGMSEEEQKALFQTATDYASRVEAALTTLAKECGCAVVNSAALLRLPEAG
                     APGIPDRTARLKELAADHK"
     gene            complement(9086..11668)
                     /gene="traC"
                     /locus_tag="PKX01_08490"
     CDS             complement(9086..11668)
                     /gene="traC"
                     /locus_tag="PKX01_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HMZ76201.1"
                     /translation="MGVLEALKTVFYGERFAPADAVPRALVEQLNQIPRLTGILPYLG
                     WLPDDRLFVLDQGAFGGKPEQNLGFCIETMPQTGSNEEMERVLASLFMSCPPGTGIQI
                     SLYAGPHILPILQKQADMLPQDEGRAPDPDSNRSDRRNRNIFRALARRRIDHYLKGTG
                     TSLFSHQVYLLRNYRCVISIATPLDPKIPADIEEAIRIRESAHATLRSAHLPGWDWQP
                     GDLLNFVADFFDHERVFGKAAVKPVEYDDSRPLRSQLSNLEIASAVADSFVKFRKAGG
                     EETAVQCFSVWQYPKYYRLGNMGALIGDYYQMALSMPCPFLITMGAVILDYESARTKA
                     QMKAARATQAAGSYMARFQPDLQDRKRDWDMVLRAFDNGRNVVQMYHQIVLLSRLPDV
                     SRVEHAVRAVWRARGFDLAKDVYLSHQALTAALPCTLTPALQKDLRMFGRVGTKTADN
                     AVMTAPLIAEWKGTETPVLTLFGRRGQILTFDLFDNKGGNFNFAVAALSGSGKSVFVN
                     EVAFRYLGCGTKVWIIDVGRSYRKLCSLVDGEFIEFSDERSNTICLNPFSMVVDINND
                     MEMLLPLIAQMASPREPLDNFGYSALAGAIKRVWDQKGKRATISDVYDLLRTGRLSDE
                     AEHERDLLRLATALEPYTRHGVYASHFEGESNIEFHKDLVVLELEELKSKKDLQAVVM
                     QLMMYRITQEMYLDRSKRKLVIIDEAWDLMSGGASSSFIEAGYRRARKYGGAFGTITQ
                     SVEDYYKNEATKAAMQNADWLFLLRQKPESIERLGKEGKLHIDEWTKRQLSSVTTEGG
                     AYSEVFVHGPMGSGIGRLILDPFSMLLYSTTAEDFQAIKTLTDRGMSVADAIESLLKQ
                     RGVH"
     gene            complement(11668..12234)
                     /gene="traV"
                     /locus_tag="PKX01_08495"
     CDS             complement(11668..12234)
                     /gene="traV"
                     /locus_tag="PKX01_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HMZ76202.1"
                     /translation="MMRRLAVAVLVPAILGGCASTMSGFDGGSQFACKAPDGVTCSSL
                     SGVYANAVANNLPALRKDGMGDAKGVPRTGDVVGRTPSSGEPIRSQPKVLRVWIAPWE
                     DADGDFHDQSYVYLVADPGRWGIEHNQKRIVDRYRPTFVQTGPAVQTQKPATSSPQAP
                     TSGVVLPGAQAYRSTGPATSPPNAGGQE"
     gene            complement(12234..12983)
                     /locus_tag="PKX01_08500"
     CDS             complement(12234..12983)
                     /locus_tag="PKX01_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HMZ76203.1"
                     /translation="MRKLALTILAFMAGSSAFAASSLDLEKRLREQYPATRITSVRES
                     PAKGIYEVVMGRNVAYTDEAGRYMVFGHLYDMKEQKDLTAAVLDELNKVDVAALPLAD
                     AIKTVRGKGERKLFVFSDPDCPYCKRLEPELAKLDNVTIYTFLYPLDGLHPDARRKAQ
                     AIWCAKDRAKAWEAFMAAGKLPEGATCDNPVERNVHLGGSLGINGTPTLIFGDGAMAP
                     GLLPAAEIERRLAAGQTAGGAVQRVKAGRGE"
     gene            complement(12997..14253)
                     /locus_tag="PKX01_08505"
     CDS             complement(12997..14253)
                     /locus_tag="PKX01_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/VirB10 family protein"
                     /protein_id="HMZ76204.1"
                     /translation="MSEESKKPLAERFKNLSPKARQYLVLGSVGTVFLGLVLGSVALW
                     DNQASVVPQAGNEERKTKNIVTPGTQVDPRDVWMAQSSQQLREMDTVIQGLKQKLADV
                     EQKQSAPQPAPFQAGVLPPLPPVPPPTPPAPSQAQPAATGADGVKPVPIAPVPPAQPR
                     EPGIAAFEVSDGANTTSQPEPEKQARTYVPSGSFMRVALLGGLDAPTGGQAQNNPWPV
                     LLRVQDNAFLPNRYRARVKECFLLGSGYGDISSERAYLRLESLSCVLNNSEVVDTPAK
                     GYVVGEDGKAGMRGRLVSKQGQVLANALMTGIIAGIGQGFQQSATTYSTSPLGSVGTV
                     EPGKQFQAGIGAGVGKALDRLSQYYITLAEKMFPIIEVDAGRIVDVVLTRGVTLGLGT
                     AIDEGDYSDIWKRGRQIMRKSLNPWE"
     gene            complement(14250..14966)
                     /locus_tag="PKX01_08510"
     CDS             complement(14250..14966)
                     /locus_tag="PKX01_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HMZ76205.1"
                     /translation="MFPISKKPVTRILSALLLLASLDAGAAQVLQGRPDDTLAASLSR
                     SEPTLIRIEGHRIRRVFGAEGDFDVKADKDAGTAFVKPMTDKAAFSAYVADDAGRTWK
                     LLLSLSDGPADSIVIKGRGGESAGKRPGKDSARSQAIKRVVLALDADGETDMESRVAN
                     ELVPLWKEALFVLVKIVQGPLGWPLRGEKYHLSNTSDKPMVIDERELYRRGVVAVSVE
                     KSELKPAETTAVYVISEATE"
     gene            complement(14899..15492)
                     /gene="traE"
                     /locus_tag="PKX01_08515"
     CDS             complement(14899..15492)
                     /gene="traE"
                     /locus_tag="PKX01_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HMZ76206.1"
                     /translation="MLFKKYLSDRDNANEEIRFLRMLVVGLVLAIVIGAGLVFRLIGA
                     EKTVVTPPEIRRSFWVSGNAVSREYLEEMAYWYAGLALNITPTVADYQNSLFLKYAAP
                     SESGRLQAEMGARAEFLKKNNTSTLFSVRNVTPDERNQRVALSGTLYTWVGDKKAPER
                     NATYLVGFQFINGRLHVSDLKETSDQNPFGTPPAGQP"
     gene            complement(15496..15792)
                     /gene="traL"
                     /locus_tag="PKX01_08520"
     CDS             complement(15496..15792)
                     /gene="traL"
                     /locus_tag="PKX01_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HMZ76207.1"
                     /translation="MGGDETGYIPKSLDAQERFLWWEMDQAILAIMLLGMGVIAGSML
                     LGMALGAATAWQYGRLKAGKHPKFAVHALYWWLPSGWFVKPRVTPPSDQRYFLG"
     gene            complement(15802..16173)
                     /gene="traA"
                     /locus_tag="PKX01_08525"
     CDS             complement(15802..16173)
                     /gene="traA"
                     /locus_tag="PKX01_08525"
                     /inference="COORDINATES: protein motif:HMM:NF041281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HMZ76208.1"
                     /translation="MNRKTLKKHAGPPQVFRRPLGGEPSAARFSGGAQFVLAVLALSL
                     VAGSAFAGTTGTEFQNLYTWLNNVVTGYFGRAVAVAAVGIGAIISIARVNPIPILAGV
                     GFAVFLQYTPTIITGILTATI"
     gene            complement(16163..16360)
                     /locus_tag="PKX01_08530"
     CDS             complement(16163..16360)
                     /locus_tag="PKX01_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76209.1"
                     /translation="MIPRSKAAVQDTLIAGAFIAMGALLPGSLLDKGFEAHVGGIALG
                     LGIGWLVKSVINHSKGVQRES"
     gene            complement(16357..16944)
                     /locus_tag="PKX01_08535"
     CDS             complement(16357..16944)
                     /locus_tag="PKX01_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ76210.1"
                     /translation="MLLMVAAGVLLIGCAGSPPVEQQDPAARRMSIGWIRHDWRICEE
                     EGSCPHPTPKTVALPAPMPTAPKPQEPAKPQPAAAAERNPAVVHFEFARATPTEAGGS
                     ELEGALAQVRQDEAIRIVGHTDDIGTAAFNARLALRRAEFVATWLKRRGVTNPMEIEA
                     HGKCCYVAANDSDQGRAANRRAEIHFTTTRKEIVR"
     gene            complement(16953..17384)
                     /locus_tag="PKX01_08540"
     CDS             complement(16953..17384)
                     /locus_tag="PKX01_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ76211.1"
                     /translation="MRKSWPILLLLILLVPPAAGNAACFEEAAARYRLPSLLLKAISR
                     VESSGRAGAINRNADGSHDIGHMQINSRWLPVLQPYGISADSLFDPCINTYVGAWILA
                     QNIARLGYGWDAIGAYNAASPSKRIAYARKVAAALERERLL"
     gene            complement(17482..18699)
                     /locus_tag="PKX01_08545"
     CDS             complement(17482..18699)
                     /locus_tag="PKX01_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76212.1"
                     /translation="MNIVGPRQAGKTTLVRDLIDSAFYTTLDDDAVREALEADAYGQL
                     LLLSEQTRSSGHPIVLDEVQRVPEITLALKRIVDADRRPGQYVLTGSSDIFTTAAAYD
                     SLAGRVMTLTLRPFSAAEIAGAAPCTLLDAVATHPADCLDVLPKPRTFTRAQAIDLIV
                     RGGFPEIRGLSDPDRMDRYRSYLDSIVERDVAPVAKVRKPDMLRRLIDQLAARTAEEL
                     NVAALCQVLGARKETVNDYLDILSRLGLVHRLGAWTSSGAKREIKAPKLHFLDTGCAT
                     ALRGEDSGSFGLDADPTAFGHLLESFVFIELEKSLPFLSKRWRLYHWRLDTREIDIVA
                     EAPGKLLALFEMKASSTVGVQDFRHIDWFRNEGPGRDYRATGFVVYLGSQLLSFGPGR
                     IALPLSMLWSFKL"
     gene            complement(18902..19501)
                     /locus_tag="PKX01_08550"
     CDS             complement(18902..19501)
                     /locus_tag="PKX01_08550"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HMZ76213.1"
                     /translation="MPRQEHQRKGEWTAFFATLLAVGIVALWALIPTQVLEATWQAEQ
                     QQMSSWAGESADRWIKAQAVEAVSAAAREAERTAAGLGDSAIERWLTDRIYASLLWAN
                     LALYRAHALLMWSLLGIPLILAASVDGFYVREIRKTAFISQSPIRHKIGVHFFRLVSL
                     AVVAWLCLPLPMPIVVAPLVVSFMALSMWLWVGHLQKRL"
     gene            complement(19509..21311)
                     /gene="traD"
                     /locus_tag="PKX01_08555"
     CDS             complement(19509..21311)
                     /gene="traD"
                     /locus_tag="PKX01_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03743.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HMZ76214.1"
                     /translation="MIGSHDFDNPWRKVYEWPMTATWLGASALTLAVTRTLPVPTRFG
                     VATSLLCAGLGAYRATQAWKRSQDTTRVRLAEKQFIDIPAMIDLARHAHRRTSLWLGK
                     GFQWTDIEASRMHALINGGLAAQLGKDALHKEGAYWLHGLAKEEDVELDLSMLEGHTL
                     IVGATGVGKTRLFDLLIAQAIVRGEPVIIIDPKGDHGLAENAKRVCEALGQPDRFIYF
                     HPAHPDRSACIDPLRNWNRKTELASRIAALIPSETGADPFTAFGWKVLNDIVNGLIAT
                     GQRPNLVQLRRYIEGGPDDLLLKALRLHFKTKVPDWESRISSFVKQYKGNQLLAYISF
                     YKEIVIHDAQAVDLDGLISTYEHNREHFQKMVASLIPILSMLTSDPLTELLSPDFEPG
                     HERQVTDMARVIRSDKVLYTGLDNLADATVGSAIGSILLADLTAVAGDRYNYGVESRK
                     PINVFVDEAAEVINQPTIQLLNKGRGAGFRMFIATQTFADFAARLGDENKARQVLANT
                     NNKIALRVLDAETQKYIADGIPKIKARTMMIRYGHNVDSNIHDTYTASYQEQATEEEA
                     DLIPPAILSELAPLHYFARLSGGRTVKGRVPILR"
     gene            complement(21308..21625)
                     /locus_tag="PKX01_08560"
     CDS             complement(21308..21625)
                     /locus_tag="PKX01_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76215.1"
                     /translation="MNVASATPEEGGYIVAMELATGAIRIAATRQPAKYVNAWRHNVR
                     RYGLPDIVRVLVSRPYLRYEAVKRGLAGLLADYKDADSEAFQVEVDVFTAKAREMFSA
                     AAR"
     gene            complement(22028..22960)
                     /gene="trfA"
                     /locus_tag="PKX01_08565"
     CDS             complement(22028..22960)
                     /gene="trfA"
                     /locus_tag="PKX01_08565"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HMZ76216.1"
                     /translation="MDHSGPQLQIDFPEPAESAANIKIAQIQDRARERQERDAIAAGL
                     PDWPENKRGLPNGALRSALFGISPKERRTYAVERHIASIDGLEVFITRGPNLMQHHLD
                     VWEQCLRLGRDQGTGRRIEFTAYAFLKSIGRNTGKSDCEWLKNALYDLAACVVRITDG
                     NRTYFGPMIHGGTRDELTGRYVIEINPKIALLYGTGRWTQLDYAQRQQLRRHPLAQWL
                     HAFYSTHAVPYRYKVETIRHLCGADKEGELWKFRQVLRRALDQLARATGWLCEIDGRD
                     CLVVRKQSESQAAIGTAGRPSEAKLSTTSTADTG"
     gene            complement(23031..>23697)
                     /locus_tag="PKX01_08570"
     CDS             complement(23031..>23697)
                     /locus_tag="PKX01_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ76217.1"
                     /translation="KLGVHRFNPYRAIAAGGDTPSLASYLKRLGYRTVCIHPYPASFY
                     QRDRVYPRLGFDEFLDIRAFDDTMRFGPYIGDAAVADKVAALLREATGPVFVFVITME
                     NHGPLHLERVAPADVEALYSAPPPDGCDDLTIYLRHLRNADRMIAKLRQTLERSERPA
                     SLCWFGDHVPIMPSVYETFGAPDGKVEFVCWSNRKAICPEVRELHAHGLALGWLRAAI
                     LVQ"
BASE COUNT         4113 a         7576 c         7555 g         4453 t
ORIGIN      
        1 agcgcgtgct tgggatgacg caggagcagt tcgctcaagc gaccggtatc catctaagcg
       61 cgatcagaaa atacgagagc cgatcgagcc tcccgagcgg tgaatcgctg ttggcgatcg
      121 ccggtaccgg catcaacctg cattggctgc tgaccaatga aggcggcatg cgtgcgcctc
      181 cagaggctgg cgcgcagcca acccaggtgt cgcccacggc cgagcttgct cagagactga
      241 cggccatcga gggtcttctg ggcggcctgg acatggacaa gcgacaagcg gtcctcaatg
      301 aaattttttc tcgcgttcag gaggcgaaac gagtagccga ccttgaggaa gtcgtcaggg
      361 ggctggaaag gaaagtgggg tagcgcttac ggcagccgat cggatggcta ccgcttcagg
      421 tgcttgaagt aggcttcccg aacgacggct gcggcggcat cgacgtcgaa cctgggcagg
      481 ctggggcgca gcaacgtatc catgtctcgc cgaaacgccc gcttgcgcag ccgctccgcg
      541 agttgggtgt tcgcttcatc ggcatccatg tgcgcaccct ccttctccag ataccatgca
      601 aacgcttcca tggctttggc gccgtcgacg gcgtagggcg tggcgcccgc ctcgctgagc
      661 tgccacgcat ggaaaaggtc gaagaggtcg cgtccctgtt cgcgctgcat caacgcccgc
      721 gtcttggtgc ccagcatctc gttgatatcg taggaacgcg ccgtcgcgcg aacgacatcg
      781 ccgtcgtcgt ccagagtatc gagttcgacg cccaccagcg ggtagagcga cgcgctctcg
      841 ttgaggttga cctcgacctt gatggtttcc tcgcgccgca atcccagcgg cacgaaccgg
      901 taggccgtgc gcaatatcct ggacggccgc aatacgttgc ggatcgacaa ccaggcgtcg
      961 gcgatcaggg aatcggccgg ctgccccagc accggcgcca ggactcggcg cagatgccga
     1021 tccagcgtgt ccgcgtccat cgtcttcacg agtaccagat cgatgtcctc ggaataacgc
     1081 gcagccggcg ccaggtgagc cttgtgcaat accgtgccac cccgcatcgc caggtactcg
     1141 cccagcaagg gatcggcgaa gatggccgcc acgccgcggg aaagccgcag atcctgttcg
     1201 atctggctgc gcctgggcca gggcgcatgg ctttgccagg cggtcaggtg cgattgctgg
     1261 atcatgcgct ggcgctgccc tgatcgggtg cgaatcgcac cttgaagagg gcatcgatca
     1321 cagccgatgt tcccggcgcc cgcccgtctt cgagcgacac gacgcgcagc agcctgccgc
     1381 gcaaccagtc ggcgacgctc gacgcgaggg cgtcctggcc atccaggttc agcaaggcgc
     1441 ccaggcgttg cgcggtgggg acgtcctgca gcgcctccag cgcctgcgac agcccctgcg
     1501 gcgtgcaagc gttccacaaa ccatgcaaga cctcgcgcac ccgcgccatg ccgccgacca
     1561 gggcttcgcg ccgcaccagg tccagcgcgg tcagctccgg cgtggcaacc ttgaaccgcc
     1621 cgtccggcgt ctcatgccag cgcgtgggca tacgctcgat gcgcgcgcag gtgtggaaca
     1681 ccacgcgatg ccggccgacc gtgatggggc gccgacgctc ggccaccatc acctgggtca
     1741 ccatcaccgc atagggcgag gcgccatgga tttcggccgc gctcaataga gcgacgtagt
     1801 agggtatccc cagcgtcttg cccagatagc ggtccagcca gatttccatt ggtggggcgc
     1861 ccgcggcggc gtactgcagc ggcacgatga gccaatgatc cgaccctcgg gacagacgga
     1921 tgagccggcc gcgccgctgc tgccgaagca gcgcttggcg gagggcctgt gcgccgacgc
     1981 tgggcagcgc cgcgcgaatg tcttccgtgc gcaagttcaa cgtgccggag cgctcggccg
     2041 catcgagaac ggccgacaaa gggctggaag cggacatgga ttcgtctcgt tcgtttcgcg
     2101 taatgtatca ttaaacgtcg cataacgcga aatgactaca gttaccctcg ggagcgccaa
     2161 aaagccgcct gctatccacg taccatagtg cggcgcatac ctatgagttg atatttgtcc
     2221 acacttggca taatgtatat tcatgaaaaa cgacaagccg ctgaattcgg agaggtgacg
     2281 aagtggcacg aacgatgact gcggagaccc tgttccagag tctccggcgc atgccagaaa
     2341 tggagcggga gcggtttttc gggctgctct gcgcgagcgc tttccgcggc gaggatctga
     2401 gccacgagga attgttctgg catctcgccg gtgacgaatt cacggcgaaa gaggcagccg
     2461 aatacctcgg agtttcgatg tcgacgttcc ggcgcttcgt cgcgtccggg aggttccatc
     2521 caagctcgac ggtcggccgc aaccagatgt ttgcggtgcc cgagttgaag gcgttcaaga
     2581 aagccttgaa gatggccagg ggctgagcct agccgtgatg cgaagaaaga ggatggcgct
     2641 cgaccatgac gactgaacaa ctgctgttct ggctggccgt attcggcaac ctgatactgc
     2701 tctaccacac gatcaagatc ggcttcctga cggtcacgct cctccggaag ggagaccggg
     2761 cgggtgctct gtggaacgcc tcggccgcaa gcgctctcgt cgggctcacg gcactgttcc
     2821 cctggacgct ggtggccgga gaacgcgaga tcggcaaggt cgcgctgttg atctgcatcc
     2881 tcacgccgtt tgtggtctcc atgaccaagc tctggccatc cgtcaaggga cggattgctc
     2941 aggcgcccat gctggcgacg gatgccgcca tcgtcggcat ggtcgtggcg ttcgccgtca
     3001 gcggcaattg ggtatggttc gggatcgctg gtatggcgat ggcggttatc tggatcgtgg
     3061 cgcgcaagtc cgcgccgagg aaactgcaga tggctgtttc tcaggaggtg cagttcgagc
     3121 cggcaaacga tccgttcgag gagttctatg ggcctgagcg ggatattgcc aatccttatc
     3181 acatcggtca ccgctgaaaa tcatcggcta ctgggtgggg gcggctcact gccgcccggc
     3241 cgcttgactc ctccatcctc ctcggcgggc tgcgttgccg ttgccggacg tgttccgatc
     3301 agttcgccga tcttcgattc tcccagtgcc aaagttctgc caagcacgtc acccccctcc
     3361 tccgcagtct tgacctcgcc cacgccggtg gagagtggag atggcgccga ctcttgtgcc
     3421 tgccgggttc cgatgacctc cccgatcttc tgctctccat gcatcctggt ctgctccagc
     3481 gcatgattca cagatccggg aagcgttgcg ctcactcccc gctcgatggc gtcgttgact
     3541 ccgaccagac tttgcctcac cggatcgcgg gggtgccctt cgcgtgaccg ctctgcagcg
     3601 gcctctgcaa cagcagatcc cggtgtcccg acgctcatat ccacgcctgg caacttctcc
     3661 atcattgcct tcgacacgcc gtcaggcatt acctgcccga ccgcattggt tgccgcgatc
     3721 cccaagagcg gctgcgtacc cggcgccgtc tgatcatgga ttttttgttc caagggccta
     3781 ccttgcgact cgaccccgcc cttgccggcg ttgagcgcgt gttgcgccct cccctgctcc
     3841 gccgccacct gctgcggcag gccgctggta acgaccttgc tcggatcgac cccggccccg
     3901 gccgcggcgc ccttcacatg accaagccat ccctgtccgt gccgcgccac atcgttcgcg
     3961 gtcggcactc cggccttgcc atgctcgaag aatgcttgca catcacccgc cgatttgacc
     4021 tcgcctacgc cggaggaaag agcgggctcg acgcgctcgt tgaagaagcg ctcgacaaac
     4081 ggtgtcatca gctcggggtt cttctccgcc atctgggcga ccgtcacagc atcgaagttg
     4141 cggcctgcaa aggtgttgta ctgagttccc atccagtccg tgaactgccg caacagacca
     4201 gtctcccacg cgcctgaatt ctcccttgcc cggctcgcgg cttccttgta ggccagcgac
     4261 tgctgatggc tggtagatgc ttgatccagg tgctgctgcg actgatcgag gcttgatcga
     4321 atacccgcca tcgctcgccg gccggattcg tcgctggtat cgaaagtcgc ttcccgtgaa
     4381 gcgcgacgta ctttgtcgac ggcctcagag aatcccgtct ctttagcgaa gttgcgggct
     4441 tcctccagga gttgcttggc ggttgtttcg gacgttccgg ccacctttgc tgcagcccgt
     4501 acgctcaccg gagaaaccag atccagggcc ttcgggttgt gcattgcgac ttcagccatt
     4561 ccaagaagct gagccgactg cgactgatcc agaccgtgct tcttcgcaaa gctgtcgaca
     4621 accttctgtg cctgattcac ggcctgcgtg aatccagcct ggtcacccag cccaaaatgc
     4681 gtacccgccc tctcgccctt cccgtgagtc cggacgaagt cggccgactg ctgcaacgcc
     4741 gccgcggtcg tggcggctgc cgacaccata ttcccgatgg ccgccgtttc ggtctgttcc
     4801 gactgctgct ggagcgcggc cgacatgcgg ctgccggcgt tgagccggac gttcatcctg
     4861 tgctgcatcc cggaggcgtc tatggccgtg gtgccatccg cgaaggtggt cgtgctggct
     4921 cctgcgccga ccagcgtcgc gaccggcatg ccagagcgca tggtcggcgc cgtgttgtac
     4981 tggccggccg tgtggagtcc cgtcttggcc gtgtccagcg tggcgttgcc catgctgagg
     5041 ttgccctgcg aggcgctcgc gccgtaggcc ccgcctgcgg tcaccgacga gaccacggaa
     5101 ttggccgccg ccatcgttgc ctgcacgatg ccgtaggcga tcacgggtat ggccaccgca
     5161 aggacgcctg ccaccgcctg gtcgctggcc acggccgcac cgaggtagtt gtagtacttc
     5221 agcgccagac cgttgccttc cgtcgcgcca gagagacctg ttgccgcctt ggtgttcatg
     5281 atgaagttcg tgacggcgta caagggcggc catagctgta cccaaaggag cgatcctaga
     5341 taggccttga ggaacgtgac ggcgaactgc cccgatatga cgatgatcag cacgacgatc
     5401 gggaagagcg cataggtcag gatctccacc gcattgcgca gcttcggcat ggtttcgtcg
     5461 gccagtgccg tcatcgtccc atacgaagtg ttgaacgatc ggatcgcctg cgcctgcgcg
     5521 agcttcacct gagcctgtgc ggcatcgccg agttgcgagg ggatcgtgta ctcggcatcg
     5581 atgatggtat tgccgacgat cgcttgccgg aggatgccct gggccgattg cgacacgccg
     5641 agcaggtagt tcgtcgtcgt cgcgagcgcg cccgtgaagg cggccttggc ggcagaggcg
     5701 ctcattcctg gataaagccg cgagccgaca gcccccagct gctgatcgac atcgagagca
     5761 agctgactgg tcagtgtcga gtaagcgccg ggacagctca ccgtattgct cgtcgctgga
     5821 tcgttgtagt ccaaaattgt gaccaggagg cccgggttgg tgttgttgaa cagcgcccag
     5881 atgtccggag cctcgctgat gtccctgctg ctgatgtaac cggacgccat gtccggccag
     5941 atgcactccc gatagaactc catcaggttg tggagcagga ggggattgac ggttccggct
     6001 gccagcttct cgcgcatgac ccgatgaccg aacagcgtcc cggtcttgcg aagctgaacg
     6061 tcatccggga gggagaacac ggtttcgaag gcagtcgtca gccagtctcc gatcttgctc
     6121 gtgccatggg ccagggatgc caagccgatc ggcacattcg ccacggtctg cgccggcgcc
     6181 gtcccggttc gatcgacgat gacgacgttg ctcttgggaa cgagcaacat cccgtggaag
     6241 atggccagca tgaacaccca gcgccagaaa tcgtcatgac gcccacgccc cgcaagaaca
     6301 ccgatcgcca gggacaggat cgccaccagc gcgagtgtgc gcagcagtcc attgaagtcc
     6361 ccgctgcccg tgatcgccgc aatcgcgttg aacaccgctg ccagctccac ggtattccaa
     6421 taggcataga cctcgaaatt caccttgggc tggcctcagc ggttgaaggt cagcaaggat
     6481 ttctgcacgt cgaccggcat cgagcgcatc atggtttcgt gcagccactg ggtcgtcttt
     6541 tgcaactcgg ccatggacac ggccttctgg tactgtgccg tgaactccgc agcggcgagc
     6601 ttcctcgcgt tggcaatttg ctcgttcagg ctcgcggcag cctgcacgat gtcctgggat
     6661 tgcgccgccg agttgttggc aagggccttc tgcagggtgc gtaccatctc ggtgaaccag
     6721 ttgtaggcaa gctccaccgc cacggtctga gagtagttgt tggtgagcag cgccggtccg
     6781 ccccggctca tcgcggcgat gttggcaagc cgccagacgg ggatcgagga gttcatgtac
     6841 acggcgtaat cgatgccgtc gaaactctgg ctctggcgac tcataatgtt gctcgtcgcc
     6901 ttttccatcg tctggcgcac gtgccatgag agcgacgcca cggattggtt cgacaccacg
     6961 gtcggattca ggcaatcggg atcgccaccg cacttgagca gggtgatgtc gttcttcgtc
     7021 gccgactcgg gattgccgat gaagtcggag aacttgattc cggtcggccc cttgtacacc
     7081 cagagcggct tttcgccgtt gctgttgtcc atgccggaac cgtagttctc gccggacggg
     7141 acgatgatga tcgtccccac catgctcatc atcagctcct tcagctcgtc gggggtatcg
     7201 gtcttccgca gcgccttcca gacgacgttc ccgggctgga ggacctcttt gaggtttgca
     7261 tcggcgttcg cggcctgctg gcgggcctgc ttcttggcgt tcttgtcctg gcgccactga
     7321 tcgaaggact ggaagctgtc cggccacagg ttcgagagca aagagcccgt cccctgggcg
     7381 ttcgcctgct ccttcttgtc cgacagcagc ggccccaggg tggccgtcgt gatgccctcc
     7441 gccatctggc acgaattgag gttcatgccg ttgatcttgg acgactggtc ctggaggtac
     7501 tggagcagat cggccaggtc gggcgaaatg gacttgaccg ccatcatgaa tgcgtaaccg
     7561 accgcgttgt tggcgatgtt cctcagcagc gcggtgagct gctcggagtt gatgaacgag
     7621 aacgatccgg cgaaaaggtc gatgccgccg catcccgcac ggatgctggg cggtgcaatc
     7681 gaggcgagct ggtagttgcg gatgggcgta cgcatgtaca tgctgccgcc ggtgtagacg
     7741 gtcgtggtct gcccctgaac cacctgggcg ccggtcacat tgccgtaggc gcccatctcg
     7801 ttgaaccagg tttgcatctc ggactggacg ctggctgtgg ccgacgcgct gaacgcgacg
     7861 atgagcacag cggcaagcag gcgtttcccg ggcatcgcga ttcctctctc aataaagctc
     7921 acccggctgg gtgccggtga ggatgtagat ccgctcgatc acgtcctgcg cggaaatcac
     7981 gccggaggcc aggggcatga ggcgcttgtc cttgacgttc ccgagcacga gcatcggtac
     8041 ttgccggatc ccgagcttct cgaccagacc gttgtcgcgc tggggattcg ggtattcggg
     8101 caggccagcg ccgtcgacgc tgacgggcag caccgtgatg ccgtaggtat ccgtcaggaa
     8161 cctgagggtc ggcgccaggc gatgacagta agggcaatcc gagcggaaga agaagaacag
     8221 cccccattcc ttgttgatgt cggaaagctg gcgcttctcg gccgccttgc gcgcgctggc
     8281 ataggtggcg atcgcggcgt tattgaccgg gcgcttcagc tcgtagttga gatcggggtt
     8341 ttgccagacc acgcgccgcc acacgtccga gaacacggag cccatgttca tcatcgcctc
     8401 ttgcgcagcg atgtaggcct tgaggttgtc cggggtggga tcgacgatcg ccagcgccct
     8461 cttcgccttc agttccttct gcaaggtctc gatgcgttcg agggcctgtt cctcccggct
     8521 cttggccttc ttcggctcgt ccggttcggt cgccggccct gggggctcgg gctccaggtc
     8581 gcaataccag ttgaacttcg agcggtcgca cgcccaaatc gatgggtagt cgatccccgc
     8641 gccaccgctt gcggcggttg ccagcgccga gccggaaccg gcggcggcca cagcgagcac
     8701 gagccaaacg ggaggtatcc acatcatttg tggtcggcgg ccagttcctt tagcctggcc
     8761 gtcctgtcgg gaatcccggg cgccccggct tccggcaggc gcaagagcgc ggcggaattc
     8821 acaacggcgc agccgcattc cttggcgagc gtcgtcaagg cggcttccac ccggctcgcg
     8881 tagtcggtgg ccgtctgaaa cagcgctttc tgctcctcct cggacatgcc gggcttgatc
     8941 cggtcggcca aggccttctt ctgttcttcg aagaggctgc ccatgtccac gcggaccatc
     9001 agcggccagg gatgggtatg aacccatgcc gccccgacaa tgcatgcgga cagcaccgcg
     9061 accgcccagg tgatgacgtg agccttcaat gcactcccct ttgcttcaac aaggactcga
     9121 tcgcgtccgc caccgacatg ccccgatcgg taagcgtctt gatcgcctgg aagtcttccg
     9181 ccgtggtgga atagagaagc atggagaacg gatcgaggat gagccggcca atgccagagc
     9241 ccatcggacc atggacgaag acctcggaat acgcgccgcc ctcggtcgtg acgctggaga
     9301 gctggcgctt cgtccattcg tcgatgtgga gcttaccttc cttgccgagc cgctcgatgg
     9361 attcgggctt ctggcgcagc aggaacagcc agtccgcgtt ctgcatggcg gccttggtcg
     9421 cctcgttctt gtagtagtcc tccacggatt gcgtgatggt gccgaaggcg ccgccatact
     9481 tgcgtgcccg ccggtagccg gcctcgatga acgagctgct tgccccgccg ctcatcaggt
     9541 cccaggcctc gtcgatgatg accagcttgc gcttggagcg gtccaggtac atttcctggg
     9601 tgatgcggta catcatgagc tgcatcacca cggcctggag gtccttcttg ctcttgagct
     9661 cctcgagttc gagcaccacc aggtccttgt ggaactcgat gttcgactcg ccctcgaaat
     9721 ggctggcgta gacgccgtgg cgggtgtagg gctccagggc cgtcgccagt cgcagcagat
     9781 cccgctcgtg ctcggcctcg tcggacagcc ggcccgtcct gagcaggtca tagacatcgg
     9841 agatcgtggc gcgcttgccc ttctgatccc acacccgctt gatcgcgccc gccagggccg
     9901 agtagccgaa gttgtccagg ggctcccggg ggctcgccat ctgggcgatc agcggcagca
     9961 gcatctccat gtcgttgttg atgtccacca ccatcgagaa ggggttcagg cagatcgtgt
    10021 tgctgcgctc gtcggagaat tcgatgaact cgccatccac cagtgaacag agcttccggt
    10081 acgagcggcc cacatcgatg atccagacct tggtgccgca gccgaggtag cggaaggcca
    10141 cttcattgac gaagaccgat ttgccggaac ccgacagcgc cgccacggcg aagttgaagt
    10201 taccgccctt gttgtcgaag agatcgaagg tcaggatctg gccgcgccgc ccgaacagtg
    10261 tcagcaccgg cgtctccgtt cccttccact ccgcgatcag cggggccgtc atgacggcgt
    10321 tgtcggcggt cttggtgcct acccgcccga acatgcgcag atccttctgt aaggccggcg
    10381 tcagcgtgca cggcagggcg gccgtgaggg cctggtggct gagatagaca tccttcgcca
    10441 ggtcgaagcc ccgtgcccgc cagacggcgc gcacggcgtg ctccacccgg gacacgtccg
    10501 gcaggcgcga gagcaggacg atctggtggt acatctggac gacgttgcgg ccgttgtcga
    10561 aagcccgcag caccatgtcc cagtcgcgct tgcgatcctg caggtcgggc tggaaacggg
    10621 ccatgtagga gccggccgct tgcgtggccc gggccgcttt catctgcgcc ttcgtccggg
    10681 ccgactcgta gtcgagaatg acggccccca tggtgatgag gaaggggcac ggcatggaca
    10741 gggccatctg gtagtagtcg ccgatcaagg cccccatgtt gcccagccga tagtacttgg
    10801 ggtattgcca cacggagaaa cactgcacgg ccgtctcctc gccgccggcc ttgcggaact
    10861 tgacgaagct gtccgccaca gcggaagcga tctccaggtt cgagagctgg gagcgcagcg
    10921 gcctcgagtc gtcgtattcg accggcttga ccgccgcctt gccgaagacc cgctcgtggt
    10981 cgaagaaatc ggcgacgaag ttgagcaggt cacccggctg ccagtcccac cccggcagat
    11041 gggcggaacg cagcgtggca tgggcgcttt cccggatgcg gatcgcctct tcaatatcgg
    11101 ccggtatctt cgggtcgagc ggcgtggcga tcgagatcac gcaccggtag ttgcgcagca
    11161 gatacacctg gtgggagaag agcgaggtgc ccgtcccctt cagatagtga tcgatacggc
    11221 gacgcgccag ggcacggaag atgttccgat tgcgtcgatc actccggttc gagtccggat
    11281 ccggcgccct gccctcgtct tggggcagca tgtccgcctg cttttggagg atgggcagaa
    11341 tgtgcggacc ggcgtacagc gaaatctgga tccctgtccc gggcgggcag gacatgaaaa
    11401 ggcttgccag cacccgctcc atttcctcgt tggagccggt ctggggcatg gtctcgatgc
    11461 agaagcccag gttctgctcg ggcttgccgc cgaaggcgcc ctgatcgagc acgaacagcc
    11521 tatcgtccgg cagccagccg agatatggca ggatgccggt caggcgggga atctgattga
    11581 gctgctccac cagcgcccgc ggcaccgcat cggcgggcgc gaaacgctcg ccgtagaaaa
    11641 ccgtcttcag ggcttccagg acgcccacta ctcctgccct ccggcgttgg gcggggaggt
    11701 tgccggccct gtggacctat aagcctgtgc gccgggaaga acgacaccgc tggtcggcgc
    11761 ctgaggcgaa gacgtggccg gcttctgcgt ttgcaccgcc gggccggtct gaacgaaggt
    11821 gggccgatag cggtccacga tccgcttctg gttgtgctcg atcccccacc gtcccggatc
    11881 ggcgacgaga tagacgtacg actgatcgtg gaagtcgccg tcggcatcct cccacggcgc
    11941 gatccagacg cgcaggacct tgggctggct tctgatcggc tctccggaag acggcgtgcg
    12001 gccgacgacg tcgccggtgc gcggaacgcc tttggcatcg cccattccgt ccttccgcag
    12061 ggcgggcaga ttgttggcga cggcgttggc atacacaccg gacagcgacg agcaggtcac
    12121 gccgtccggg gccttgcagg cgaactggct gccgccgtcg aagcccgaca tcgtgctcgc
    12181 gcagccaccg aggatggccg gaaccaggac cgcaacggcg agcctgcgca tcatcactct
    12241 cccctccccg ccttcacgcg ctggaccgcc ccgcctgccg tttgccctgc ggccagtctc
    12301 cgttcgatct ccgccgccgg cagcaggccc ggggccatgg ccccgtcccc gaaaatgagc
    12361 gtgggcgtgc cgttgatccc gaggcttccg cccaggtgca cgttgcgttc caccgggttg
    12421 tcgcaagtgg cgccttcggg gagtttcccg gccgccatga atgcctccca agccttggcc
    12481 cgatccttcg cgcaccagat cgcctgagcc ttccgtctgg cgtccggatg gagcccgtcg
    12541 agcggataga ggaaggtgta gatggtcacg ttgtcgagct tggcgagctc cggctccagc
    12601 cgcttgcagt aggggcagtc cgggtcggag aagacgaaca gcttgcgctc gcccttgccg
    12661 cgcacggtct tgatggcgtc cgccagcggc agcgccgcga catcgacctt gttgagttcg
    12721 tcgaggacag cggcggtcag gtccttctgc tccttcatgt cgtaaagatg gccgaacacc
    12781 atgtaccggc ccgcctcgtc cgtataggcc acattgcggc ccatgacgac ttcgtatatg
    12841 cccttggctg ggctttcccg gacgctggtg atccgcgttg ccgggtattg ctcgcgaagg
    12901 cgcttttcca ggtcgaggga ggaggcggcg aaggccgagg agccggccat gaaagcgagg
    12961 atcgtgagcg cgagtttccg catgttggac tgttccttat tcccaggggt tgagcgattt
    13021 gcgcatgatc tggcgaccgc gcttccagat gtccgagtag tcgccttcgt cgatggcggt
    13081 gccgaggccg agggtcacgc cccgggtcag gacgacgtcg acgatgcgac cggcgtcgac
    13141 ctcgatgatc gggaacatct tctcggccag cgtgatgtag tactgggaga gccggtccag
    13201 cgccttgccg acgccggcgc cgatgccggc ctggaactgc ttgcccggct cgaccgtgcc
    13261 cactgagccg agcgggctgg tgctgtaggt cgtcgcgctc tgctggaagc cctgcccgat
    13321 gcccgcgatg attccggtca tcagggcatt ggcgagcacc tggccttgct tgctgaccag
    13381 ccgcccgcgc atccccgcct tgccgtcctc cccgacgacg taacccttgg caggcgtatc
    13441 gaccacctcg ctgttgttga ggacacagga gagagattcg agccgcaggt acgcacgctc
    13501 cgagctgatg tcgccgtagc cggagccgag caggaagcac tccttgaccc tggcccgata
    13561 gcggttgggc aggaaggcgt tgtcctgcac gcgcagcaag accggccaag ggttgttctg
    13621 cgcctggccg ccggtgggcg cgtcgagccc gccgagcagc gccacgcgca tgaaggaacc
    13681 ggaggggacg taggtcctgg cctgcttctc gggttccggc tggctcgtgg tattcgcgcc
    13741 atcgctgacc tcgaacgcgg cgataccggg ctccctcggt tgcgccgggg gaacaggtgc
    13801 gatgggcacg ggtttcacgc cgtcggcgcc ggtcgcagcc ggttgagcct gggacggggc
    13861 cggcggcgtg ggcgggggca cgggaggcag cggcggcagc acgccggcct ggaagggcgc
    13921 cggctgggga gcagactgct tctgctcgac gtccgccagc ttctgcttca ggccctggat
    13981 gaccgtgtcc atctccctga gctgctggga ggattgggcc atccagacat cgcgcgggtc
    14041 cacctgcgtg ccgggcgtaa cgatattctt cgtcttgcgc tcttcgttgc cggcctgagg
    14101 gaccacggag gcctggttgt cccagagggc gacgctgccg agaacgagcc ccaggaacac
    14161 ggtccccacc gagcccagca cgaggtactg cctggccttg ggcgacagat tcttgaagcg
    14221 ctcggcgagc ggcttcttgg actcctcgct cattccgtag cctccgaaat cacgtagacc
    14281 gccgtcgtct ccgcgggctt gagttcggat ttctcgaccg acaccgcgac cacgccgcgg
    14341 cggtagagct cccgctcgtc gatcaccatg ggcttgtccg acgtgttgct caggtggtac
    14401 ttctcgcccc tcagtggcca cccgagcggc ccctggacga tcttcaccag gacgaacagg
    14461 gcttccttcc agagcggcac cagttcattc gccacccgtg actccatgtc cgtttcgccg
    14521 tcagcgtcga gggccaggac aacccgcttg atcgcctggc tacgcgccga atccttgcca
    14581 ggccgtttgc cggcggactc cccgcccctt cccttgatga cgatcgagtc ggccggcccg
    14641 tcggacaggg acaacagcag cttccaggtc cgtccggcat cgtccgccac ataggcgctg
    14701 aaggccgcct tgtcggtcat gggcttcacg aacgccgtgc ctgcgtcctt gtcggccttc
    14761 acgtcgaagt cgccctcggc gccgaacacg cgccggatgc gatgcccctc gatgcgaatc
    14821 agcgtgggct cggagcgcga caggcttgcc gcgagcgtgt cgtcgggacg cccctgcaga
    14881 acctgggccg cacccgcgtc aaggctggcc agcaggagga gtgccgaaag gattctggtc
    14941 actggtttct ttgagatcgg aaacatggag tcgtccattg atgaactgga acccgacgag
    15001 gtaggtggca ttccgctcgg gggctttctt gtcgcccacc caggtgtaga gggttccgga
    15061 gagcgccacg cgctggttgc gctcatccgg cgtgacgttg cgcacggaga acaacgtgga
    15121 cgtgttgttc ttcttcagga actcggcccg cgcccccatt tccgcctgca ggcgcccgga
    15181 ttcggacggg gccgcgtact tgaggaacag gctgttctgg tagtccgcga cggtcggcgt
    15241 gatgttgagc gccaggcctg cgtaccagta cgccatttcc tccaggtact ccctggaaac
    15301 ggcattcccc gaaacccaga aggagcgtcg gatttccggc ggcgtgacga cggttttctc
    15361 ggcgccgatg agccgaaaga cgagcccggc accgatgacg atcgccagta ccaggccgac
    15421 gaccagcatc cgcaggaagc gaatctcctc gttggcgttg tcccggtccg agaggtattt
    15481 cttgaagagc atcgcctacc ccaggaagta gcgctggtcg gacggcggtg tgacgcgggg
    15541 cttcacgaac cagccgctcg gcagccacca gtagagcgca tggacggcga acttcggatg
    15601 cttgccggcc ttgagccggc cgtactgcca tgccgtagcc gcaccgagcg ccatgcccag
    15661 cagcatggag ccggcgatga cgcccatgcc caggagcatg atggcgagga tcgcctggtc
    15721 catctcccac cacaggaagc gctcctgcgc gtcgagggac ttggggatgt agccggtttc
    15781 gtcgccgccc atgccgcacc ctcagatcgt cgccgtcagg atgccggtga tgatcgtcgg
    15841 cgtgtattgg aggaagaccg caaaaccgac acccgcgaga atcgggatcg ggttgacgcg
    15901 cgcgatcgat atgatcgcgc cgatgccgac ggctgctacc gcgacggccc ggccgaagta
    15961 gcccgttacg acgttgttga gccaggtgta gaggttctgg aactccgtcc cggtcgtgcc
    16021 ggcaaaggcg ctgcccgcga caagcgagag cgccagaacg gcgaggacga attgagctcc
    16081 ccccgaaaac ctcgctgcgc tcggttctcc ccccaggggg cgacgaaaca cttgaggcgg
    16141 ccctgcgtgt ttcttgaggg tcttacgatt cacgttgaac tcccttcgag tggttgatga
    16201 cggatttgac gagccagccg atgccgaggc ccagcgcgat gccgccgacg tgggcttcga
    16261 atcccttgtc gagcagcgat ccgggaagca gtgctcccat cgcgatgaag gctccggcga
    16321 tcagcgtgtc ctggacggct gctttcgagc gaggaatcac ctgacgatct ccttccgagt
    16381 ggttgtgaaa tggatttccg cgcggcgatt ggcggcgcgg ccttggtccg agtcgttcgc
    16441 cgccacgtag caacacttgc cgtgggcctc gatttccatc ggattcgtaa cgccccgacg
    16501 cttgagccac gtcgccacga actccgcccg gcgaagggcg agccgagcat tgaaggcagc
    16561 agtgccgatg tcgtcggtat ggccgacgat gcggatcgcc tcgtcttgcc gaacctgggc
    16621 gagcgcccct tcgagttccg aaccgccggc ctcggtcggg gtcgcccgcg cgaactcgaa
    16681 gtgcacgacg gccggattgc gctccgccgc cgcggccggc tgcggcttcg caggttcttg
    16741 cggcttgggg gccgtcggca tcggcgccgg cagcgcgacg gtctttggcg tcggatgcgg
    16801 gcaggagccc tcctcctcgc agatccgcca gtcgtgcctg atccagccga tgctcattcg
    16861 ccgcgcggcc ggatcctgct gttcgaccgg tggactgccg gcgcagccga tcagcagcac
    16921 gccggccgct accatcaaca gcattgcgcg aatcaaagca gccgctcccg ttcgagagcc
    16981 gctgcgacct tccgcgcgta ggcgatccgc tttgacgggc tggctgcgtt gtaagccccg
    17041 atcgcatccc agccatatcc cagccgggca atgttctgcg cgagaatcca tgcgccgaca
    17101 taggtgttga tgcagggatc gaacagcgag tcggcgctga tgccataggg ctggagaacg
    17161 ggaagccacc ggctgttgat ctgcatgtgg ccgatgtcgt gactgccatc ggcgttgcgg
    17221 ttgatggcac ccgcacggcc ggacgactcc actcgggaaa tcgccttgag gagcaaggac
    17281 gggagccggt agcgggccgc ggcctcctcg aagcacgcgg cgttaccggc agccggaggg
    17341 accagcagga tcaggagaag gagaatcggc catgacttgc gcatggccag gatgctacga
    17401 attcagagcg tcaaaacgac tgcgaaaagc acacagaagc gaaccgtttc gctccgtcgg
    17461 cgcaggaggc gcctcagccg gtcatagctt gaatgaccag agcatcgaca atggcaaggc
    17521 aatccggccg gggccgaagg acagcagctg gctgccgagg tagacaacga agcccgtggc
    17581 gcgataatcg cgccccggcc cctcgttccg gaaccaatcg atgtgacgga agtcctgcac
    17641 cccgacagtg gaagaggcct tcatctcgaa cagcgccagc agctttcctg gcgcctcggc
    17701 cacgatgtcg atttcccgcg tatccagccg ccagtggtac agacgccagc gcttcgacag
    17761 gaagggcagg gacttctcca gctcgatgaa gacaaagctt tccagcagat gaccgaatgc
    17821 ggtgggatcg gcgtccagac cgaagctccc ggagtcctcg ccccgcagcg ccgtggcgca
    17881 acccgtatcc aggaaatgca gcttcggcgc cttgatctcc cgcttcgctc ccgatgatgt
    17941 ccatgccccc agccgatgca ccagccccag gcgggacagg atatcgagat aatcgttcac
    18001 cgtctccttg cgtgcgccaa gcacctggca aagcgccgca acattcagct cttcggccgt
    18061 gcgcgccgcg agctggtcga tcaggcgccg cagcatgtcc ggcttgcgga ccttggcgac
    18121 gggagcaaca tcccgttcca cgatgctgtc gagatagctc cggtaacggt ccatgcggtc
    18181 gggatcgctt aagccccgta tctccgggaa tcccccgcgc acgatcagat cgatcgcctg
    18241 ggcacgagtg aaagtacggg gcttgggcag gacatccaga caatcggccg gatgcgtggc
    18301 caccgcatcg aggagcgtgc aaggcgcggc ccccgcgatc tcggcagcgg aaaacggcct
    18361 gagggtcagg gtcatgacgc gcccggcaag tgagtcgtag gcggctgccg tggtgaagat
    18421 gtccgacgag cccgtcagga catattgtcc gggccgccgg tcggcatcga cgatccgctt
    18481 caaggcaaga gtgatttccg gtacccgctg cacctcatca agaacgatgg ggtgcccact
    18541 gctcctcgtt tgctcagaga ggagaagaag ctgaccatac gcatcagcct caagggcttc
    18601 ccggacggca tcgtcgtcga gcgtggtgta aaacgccgaa tcgatcagat ctcgaacgag
    18661 cgttgtctta cctgcttgac gcggcccgac gatgttcacg acccgactcg cgcccagtgc
    18721 acgttcaatc cgtgccgcga gatgtcgctg tatgatttgc atccgccata cccctgcaat
    18781 acgactatta tgattccagc aaaaaggcta tcacaacaag tgcagtaagg ctagtaacta
    18841 tctggataat aggctagtcg ccatgaagcg cctgacgcat atgcctccct tcgccggccc
    18901 gtcatagccg cttttgcaag tggccgaccc acagccacat cgacaaggcc atgaaactga
    18961 ccaccagggg agcgacaacg atcggcatcg gaagcggcaa acaaagccac gccacgaccg
    19021 cgaggcttac gaggcggaag aaatggacgc cgatcttgtg gcggatggga ctctgcgaga
    19081 tgaacgccgt cttgcgaatc tcgcgcacgt agaagccgtc cacggatgcc gcgaggatca
    19141 gcgggattcc aagaagcgac cacatcagga gcgcgtgggc gcgatacagg gcgagattcg
    19201 cccacagcag gctcgcgtag atcctatccg tgagccatcg ttcgatggcg ctgtcaccca
    19261 ggcctgcggc tgtccgctct gcctccctgg cggctgcgct caccgcctcc acggcttgag
    19321 ccttgatcca gcgatcggcg gattcccccg cccacgacga catctgctgc tgttcggcct
    19381 gccatgtggc ctccagcact tgcgtcggaa tcagagccca cagcgcgaca atgccgacgg
    19441 ccagcagcgt ggcgaagaaa gcggtccact cgccctttct ctgatgttcc tggcgcggca
    19501 tttttttctc acctcaagat cggcacccgg cccttgaccg tcctgccgcc ggacagccgg
    19561 gcgaagtagt ggagcggcgc cagctcgctc aagatggccg gcggtatcag gtcggcctct
    19621 tcttccgtcg cctgctcctg gtaggacgcg gtgtacgtgt cgtggatgtt ggagtcgacg
    19681 ttgtgaccgt agcggatcat catggtccgc gccttgatct tcggaatgcc gtcggcgatg
    19741 tacttctgcg tctccgcgtc gagcacgcgc agcgcgatct tgttgttggt gtttgccagc
    19801 acctgccggg ccttgttctc gtccccgagg cgcgcggcga agtcggcgaa ggtctgggtg
    19861 gcgatgaaca tgcggaagcc ggcgccgcgg cccttgttga gcaactggat cgtgggctgg
    19921 ttgatgacct cggccgcctc gtcgacgaag acattgatcg gcttccggga ttccacgccg
    19981 tagttgtagc ggtcgccggc gacagccgtc aggtcggcca gcaggatcga gccgatggcg
    20041 ctgcccaccg tggcgtcggc caggttgtcg agaccggtgt agagcacctt gtccgatcgg
    20101 atcacgcggg ccatgtcggt gacctgcctt tcgtggccag gctcgaaatc cggcgacagc
    20161 agctccgtca agggatcgct ggtcagcatg gacaggatcg ggatgaggga ggccaccatc
    20221 ttctggaagt gctcccggtt gtgctcgtag gtcgagatca aaccgtcgag gtccacggcc
    20281 tgggcgtcgt ggatgacgat ctccttgtag aagctgatgt aggccaggag ctggttgccc
    20341 ttgtactgct tcacgaagct gcttatccgc gactcccagt cgggcacctt ggtcttgaag
    20401 tggaggcgca acgccttgag aagcaggtca tcgggtccgc cctcgatgta gcgccgcaac
    20461 tgcacgaggt tgggacgctg accggtggcg atcaggccgt tgacgatgtc gttcaacacc
    20521 ttccagccga aggccgtgaa ggggtcggca ccggtttcgg acgggatcag cgccgcaatc
    20581 cggctggcga gctccgtctt gcggttccag ttgcgcagcg gatcgatgca ggcggatctg
    20641 tccgggtggg ccgggtggaa gtagatgaac cgatcgggct gtccgagtgc ctcgcagacc
    20701 cgcttcgcgt tctctgcgag accgtggtcg cccttcgggt cgatgatgat gacgggttcc
    20761 cctctcacga tcgcttgggc gatgaggaga tcgaagagcc gcgtcttgcc cacgcccgtg
    20821 gccccgacga tcagcgtgtg gccctccagc atggaaaggt cgagctcgac gtcttcttcc
    20881 ttcgccaggc cgtgcagcca gtaggccccc tccttgtgca gagcgtcctt gccgagctgc
    20941 gccgccagcc cgccgttgat gagggcgtgc attcggctcg cctcgatgtc ggtccactgg
    21001 aaccccttgc cgagccaaag cgaggttctc cggtgcgcat gccgggccag gtcgatcatc
    21061 gccggaatat cgatgaactg cttttcggcg agacgaacgc gcgtcgtgtc ctggctgcgc
    21121 ttccatgcct gggtggcccg gtatgcaccc agtccggcgc acagcaacga ggtggccacg
    21181 ccgaagcggg tcggcaccgg cagcgtcctg gtgacggcca gggtgagcgc cgacgcgccc
    21241 agccaggtgg ccgtcatcgg ccattcgtag accttgcgcc acgggttgtc gaagtcgtgg
    21301 gaaccgatca ccttgccgcc gccgaaaaca tctcacgggc ctttgccgtg aagacatcga
    21361 cttcgacctg gaaggcttcg gagtcggcat ccttgtagtc ggcgagtaga ccagccagcc
    21421 ctcgcttgac ggcctcataa cgcagatagg gacgggacac gagcacccgg acgatgtcgg
    21481 gcaggccgta acgccggacg ttgtggcgcc aggcgttcac atacttggca ggctggcggg
    21541 tggctgcgat acggatggcg cccgtcgcaa gctccatggc gacgatgtaa ccgccctcct
    21601 cgggggttgc ggatgccaca ttcacgtcga cgaactcagg cctgccgtcg taaagccatg
    21661 cctgccgcga ctttttgcgc ctggtattca tggccggcaa ggtaccaagc gcgcttcgca
    21721 tggcacggct gaaaagcgca ccaaaccggc atttttggcg ccctggccga aactctcccg
    21781 gcctctctat ctttaagaag aagattagag aaagattatg ccgcttgtgg acaagtcgcc
    21841 cttgccttgc ctggcaaggg ttttgcggcg actgcaaccg gcaaagccaa cccgccatcc
    21901 cgtgagatcg ctacccgctg tcccgacagc tttctacccg acatcccatg ccgtgctacc
    21961 cgccatcccg tgatccgtgg tttgtcacct acccgcgatc ctgcgatttc cctttccgac
    22021 ctacccgcta tcccgtgtcc gccgtcgacg ttgtggataa ctttgcctcc gatggcctac
    22081 ccgctgtccc gatagccgcc tggctttcgg attgcttccg gacgaccagg caatcccttc
    22141 cgtcgatctc gcagagccac cccgtcgccc gggcgagttg atcaagcgcc cgtcgcaaga
    22201 cctgcctgaa cttccacagc tcgccttcct tgtcggcccc gcacaggtgt ctgatggtct
    22261 ccaccttgta gcggtatggc acggcatgcg tcgaatagaa agcatggagc cactgcgcca
    22321 acggatgcct tcggagctgc tgccgctgcg catagtcgag ttgcgtccac ctgccggtgc
    22381 cgtagagcag cgcgatcttg gggttgatct cgataacata acggccagtg agttcatccc
    22441 gcgtgccgcc gtggatcatc ggaccgaaat aggtacggtt gccgtccgtg atgcgcacca
    22501 cacaggcagc cagatcatac agggcgttct tgagccactc gcagtcgctc ttgccggtgt
    22561 tccggccgat ggacttgagg aatgcgtagg cggtgaactc gattcgcctg cccgtcccct
    22621 ggtccctccc gagcctcagg cattgctccc acacgtcgag gtgatgctgc atgagattcg
    22681 ggccgcgggt gatgaaaacc tccagcccgt cgatggaagc gatatgccgc tctaccgcat
    22741 aggtgcgccg ctccttgggc gagatcccga acagggccga cctcagggcg ccgttcggca
    22801 ggccgcgctt gttttcgggc cagtccggga ggcctgccgc gatcgcatcc cgttcctggc
    22861 gctcgcgggc ccggtcctgg atctgggcga tcttgatgtt tgcagccgat tcggcgggtt
    22921 cggggaaatc gatctgcaac tgcgggccgg aatgatccat cccgcggttg tatcaaggcg
    22981 cggggtggaa acgggggcga aaagcacctt tggcagcagc gtttccaaaa ctactggaca
    23041 agaatcgccg cgcgcagcca gcccaaggcc aggccatggg catgaagctc gcgaacttcc
    23101 ggacagatcg ccttccggtt gctccagcaa acgaactcga ccttcccatc aggcgcgcca
    23161 aaagtctcat agaccgaagg catgatcggc acatggtcgc cgaaccagca caggctggcc
    23221 ggtcgttcgc tccgttcaag cgtctgacgc agtttcgcga tcatccggtc tgcattgcgc
    23281 aagtggcgca ggtagatggt cagatcgtcg cagccatccg gcggcggagc ggaatacaac
    23341 gcttcgacat cggcaggggc cacccgttcg aggtgtagcg gcccgtggtt ctccatcgtg
    23401 atcacgaaga cgaacacagg acctgtggct tcacgcaaga gcgcggcaac cttgtcggcc
    23461 acggcggcgt cgccgatgta agggccgaag cgcatcgtgt cgtcaaatgc gcgaatatcc
    23521 agaaattcgt cgaaacccaa acgcggataa acgcggtcac gctgatagaa gctcgcgggg
    23581 tagggatgga tgcagaccgt gcgatagccc agtcgcttca ggtaagacgc cagggatggg
    23641 gtatcgccac cggccgcgat cgcccgatac gggttgaagc gatgcacgcc gagcttg
//
LOCUS       DAOQPA010000048        23530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1029_length_23530_cov_17.637998, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000048 DAOQPA010000000
VERSION     DAOQPA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23530
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_23530_cov_17.637998"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PKX01_08575"
     CDS             complement(<1..781)
                     /locus_tag="PKX01_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HMZ76218.1"
                     /translation="MPVHHDAFISYSHTADGALAPAFQRGLEQLAKPLLRLRAQKIFR
                     DQTDLTASPALWPGIVSHLAASDWLLVFANPLYAGSRWCRKEVEWWLAERSPERLLIV
                     LTDGDIVWDEARRDFDWTRTTALPDCLAGHLADEPLYVDLRWARETPGLSLAHGGFRE
                     AVVRVAAPLRGVSPTDLDGADLRQLRRTRLFVRTGVGAICVAAGVAVWQARVATEEAR
                     IAETQRQQAEAQRRIAESERAQAEAQRHIAESERARAEAALA"
     gene            complement(853..1815)
                     /locus_tag="PKX01_08580"
     CDS             complement(853..1815)
                     /locus_tag="PKX01_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMZ76219.1"
                     /translation="MTAQTPPRSVFHAGEEALQALAGAQARLAETGPRVIREVLPEQH
                     RSFFAELPFAVLGALDADGQPWATAIAGPPGFITSPTPERLRIGALPAADDPAAAGLA
                     PGAAIGLLGLQPHTRRRNRANGRVAALDADGFTLAVEQSFGNCPKYIHARRVEHAAQT
                     AAPAPARVQARLDGATADLIRHADTFFIATAHPDAARGEHAAHGVDVSHRGGRPGFVG
                     VDGDHLWVPDFAGNQYFNTLGNLLLNPRAGLLFVNFSEGSLVWIAATAQILHDGRAVD
                     AYPGAQRVLQLTVHALWTARRRLPLHSQDGEASPFLAGTGIWPG"
     gene            complement(1822..2454)
                     /locus_tag="PKX01_08585"
     CDS             complement(1822..2454)
                     /locus_tag="PKX01_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HMZ76220.1"
                     /translation="MNTPAQPIRFHRFATSGHSHRAELMLSLLGLPVEPIDVDLAGGA
                     QKTPAYLAMNCFGQVPVIQDGDVTVADSNAILVYLASVYDDGRWLPRDPAAAAQVQRW
                     LSVAAGQLAYGPCAARLITVFGARFNADEVIARAHALFKILDAELATRAYLTGDAPTI
                     ADVALYSYTAHAPEGNVSLDPYPHIRAWLARIEALPGFVPMPATAAGLRA"
     gene            2562..3482
                     /locus_tag="PKX01_08590"
     CDS             2562..3482
                     /locus_tag="PKX01_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76221.1"
                     /translation="MDKLKAIGTFVRIADEGSLSGAARVLDMSLPAVVRALAALEADL
                     GVRLFNRSTRRIALTEEGRRYLDSCRNLLAALDEAESGLRDEGGEPSGALTVTAPVLF
                     GQLFVAPAITRFVQRFSRVHCRVLLFDRIVNLLEEGVDVGIRIGVLEDSTLVAHPVGH
                     VRRMVVASPDFLARQGVPAHPRDLLRANCIGFSPTPGVWWRFEEQGRSFGVPVRGNLE
                     FNHVAPALDACIAGLGFGMFISYQVAPHLAAGRLVPVLEAFEQAPRPVSVVVPHARLL
                     PLRVRAFVDWVRADLSAHLRDAPGTPGSTA"
     gene            3620..5668
                     /locus_tag="PKX01_08595"
     CDS             3620..5668
                     /locus_tag="PKX01_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76222.1"
                     /translation="MKKAYLASTFVLISAPSLADAPADPGEIRNLPLSGFVDQLSLEQ
                     LGNLVVTDTKLAQAADTVTQRIVVLRNEDIERQPAHSGNLAELMRPTSGQFVNVLSRN
                     DANWGSYAGLGPKYNSYLLDGLPIDSFVDAMSLDPSAIERVEVHKGPASVLYSNYLTM
                     DFVGNETPLAGTTNFVLKSRVDTPLTRVAAGAGSWGTVFGKAYAQGTQGNLGYIVSAN
                     LEHSDYTQYGTRNSWLQTTQAPAYQKNRFFGNFSYAFDRPDHTVTVFYQRTLHQGTMG
                     RPNRGFDHTYDTLNFAYSNTFSDDWNIQFKYGERRYDRRFANDNYPTDLSLSNHEFTR
                     QTIRPADLTLSHRHWGESLLTAGVDGQWVDYQTDVQDPVGAVTPSNRAQARSTGYFVQ
                     EKLVWDAWVLRGGVRHDTIHHDYDLLGGITPATRSAAWSKNLWSFGTRYNADPGLAFY
                     GNVGTSFMAPAAKQIGGTVAVPTASGELPNPGLKPETGVGRDVGVNWRASQALTLDLR
                     GFLNTINDAIVTNIVSAAPSQVRSENAGKARAMGYEVDLRYAASSETSVFGNLTRTHT
                     RVTDPTNADHDGTAIPFAPDTLANAGVLASLPGDMSVATYYHWVGRYYDSTSRSARLG
                     FGQYGVVNVRVQKPLYRDARAAVHLTVDLNNLTDRHHAMPFDFRDPGFNGFAGVDVRY
                     "
     gene            5688..6236
                     /locus_tag="PKX01_08600"
     CDS             5688..6236
                     /locus_tag="PKX01_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HMZ76223.1"
                     /translation="MRASVRRRFRLVPLLRRLLVCAAALPLMAFSAPGELEAKIKAAY
                     VFHVIKFVDWPVLPTDALRLCVSGSDPVGVLLGELASKQVKDRPLRVDIDNGFDPAQC
                     QVLFVGRGERRWADLQARLRGGSVLTISDQDGFARIGGVVGFYSDGGRIRLEINPDAA
                     RNANLRISAKLLEVARTVPQDR"
     gene            6328..9762
                     /locus_tag="PKX01_08605"
     CDS             6328..9762
                     /locus_tag="PKX01_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76224.1"
                     /translation="MRALFEGMQIKAKLVLIIVITSVAALLIEAVGFIAYERVRVTDE
                     LVRDLSSLARIVADRSSAALLFYDDKVAMETLGALRIKRAVSAACIYDVGGRVFARYE
                     RGEDRPFSFPADPKFESRSYTEGGYLHLIEPILVEGTPQGAVFIRASFRELDLLWRDY
                     LLFAALTAFVTSMITLLVATRWQRIVSRPIERLTRTVQMVAISKDYSVRATLDSDDEI
                     GTLVEVFNGMLAAIEDRDTVLLDANRRLSENEAQLKSVNEALEQRVRERTAELQALSD
                     SASVGIVLLKDGLIVRSNRRLDEMFGYAPGEQTGRPLRDRLADTVDEEAEALAAARLA
                     AGDTVRRELMARHKDGSAFWIRLSARVIDRADPARGAVCVIEDITAERDAMDEMIKAK
                     TMAEEASRMKSDFLATMSHEIRTPMNAIIGMLYLALKNEPPAALRNYLAKAQGAAHSL
                     LGIINDILDFSKIEAGKLEIESIEFGLDTVLEQLKDSVGLQAEQKGLEFLIRYDVNIP
                     PALLGDPLRLGQIMLNLCSNAIKFTEQGEVELGFRCVAMTETDLELQFSVRDTGIGIA
                     PDVAERLFQKFTQADQTTTRRFGGTGLGLAISRRLAELMGGRLWIEQSAPGKGTTVCC
                     SIHLQIARDAQTHRRRLVEQAGPLLDGVRVLVVDDNEVSREIMAEMLRFFHLDVSVAG
                     SGAEALERLQVPDAAPVDLVLMDWRMPPMNGDEVTRRIHASPLIRHKPKVVMVTAYGR
                     EDVIKSAEQAGVAGFLVKPVSPSTLLDTILSNLGRGRILGKESSRSERGGACDFRGAR
                     LLLVEDNDINREFATELLRSMNTEVECAVNGEEAVAMVQRAPYAAVLMDIHMPVMDGL
                     EACRRIRALGQQPGGEHLGELPIIAMTALAMAHDEQRSLAAGMNDHITKPVSPERLRA
                     ALSRWLNIPLGEGGAPGPVPSSAPGSDLRALRSLDAEQGIRRIGGDVEAYRKQLRRFR
                     QRYDTAAERLRDLVAHHTPRQAEEYCHALKGVCGNIGADALFACVSDIDNLLKQEIRP
                     DEASLARMAELLATVMADIDSLAAPEQAGAPAGGGLADTDVLARVGRLRGLLESDVGA
                     AQSVLAELLAGIAGSPLEAAVREVAERVDVFEIDEALGLLQALQTQLEGRTGATPP"
     gene            9759..12677
                     /locus_tag="PKX01_08610"
     CDS             9759..12677
                     /locus_tag="PKX01_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76225.1"
                     /translation="MTSQDEAKPRILIVDDINDNLHTLMNILRGEFAVVVATNGPKAL
                     ELAVRTPPPDLVLLDIRMPGMDGYEVLHRLKSDPGTADIPVIFVTGLSDAADEAVGLR
                     LGAADYVTKPINPELLLVRVRNQLELRTHRRKKAVEGLGAAVMARSDKPALLLVDDVP
                     ENLHGLVAALQDDYRIMVAPTGAKALELVAGVNPPDLILLDILMPQMDGYEVCRRIKA
                     TPVGNRIPVIFVTVVDSVVDKVQGFSIGAADYITKPFDIDEVRARVRTHLDLSRLQRS
                     LEKLVVDRMGALQEANDALAESKAKYQILAEYSPNWEYWVAPTGGYLYVSPACRDVSG
                     YGAVDFIADPDLMRKMIHPDDLYIWDAHNERSPESGGHRLPNFRIAARDGSERWIEHV
                     CQPVIDDRGRFLGTRGTNRDVTQQVAAEEQLHLAATVFANTSEAVMITDADNRVLTVN
                     PAFVEITGYSQDDTAGREPLVLRADVMEVDAHRAMWAAIHATGRWQGEINGHCRDGRD
                     FIGWVTVSTVFKDDGSVNRRVVLFSDITEKKRADNLIWTQANYDHLTRLPNRRLFNDR
                     MEREIKKAQRKASVIGLLFIDLDRFKEVNDTQGHEVGDLLLVEAASRIRHCVRDYDTV
                     ARLGGDEFTVILSDITEGTDIGRVARSIIDRLSEAFVLNAQEHFVSASIGVALYPRDG
                     ATISELIKHADQAMYLAKDAGRSRFRFFTRALQESAEHRMRLAGDLRRALEAGQLEVY
                     YQPIVDLDTGRIAKAEALLRWRHPAHGFVSPATFIPIAEDTGTIHEIGEWVFAQVIQQ
                     ASRWRERLAPEFQVSLNMSPVQFNGRNPTQWLELLQTTQLPGSNLVIEITEGLLMSSD
                     PGIKETRLGLRDLGVGVAIDDFGTGYSSLAYLKKFDIDVLKIDQSFTRNLDPNSADFA
                     LCEAIVVMAHKLGLKVIAEGVETDQQRNLLRQIGCDHAQGYLFSRAVPAGQFEALCAR
                     "
     gene            12709..13137
                     /locus_tag="PKX01_08615"
     CDS             12709..13137
                     /locus_tag="PKX01_08615"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ76226.1"
                     /translation="MTCFSTSRHFARTPAEVFAAVRDPERLARWWGPDGFTNRFERFE
                     FQPGGCWYFTMIGPDGTAYPNQAEFTRIEPECCVVIRHTCAPHFTLTLTFEPEAGGTR
                     LHWAQAFDDPAVAEALRAIVVPANEQNLDRLAAVLGLTPA"
     gene            13257..13613
                     /locus_tag="PKX01_08620"
     CDS             13257..13613
                     /locus_tag="PKX01_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ76227.1"
                     /translation="MELLDAAEVLAALGHESRLAIFRLLVEAGPAGLNVSAIAERMGG
                     VPGATLSFHLSHLSRVGLIKGERQSRFIHYSAEYATMDELIAFLTRNCCQGKACLPKT
                     AQCDTTAKRRAAPADT"
     gene            13674..14087
                     /locus_tag="PKX01_08625"
     CDS             13674..14087
                     /locus_tag="PKX01_08625"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HMZ76228.1"
                     /translation="MNLLKTVLVLCTGNSCRSQMGEAILNYELAGVVRALSAGTAPQP
                     KVADGALEALRLAGLPTEGLHPKTVEAVMDQDIDLVVSVCDNAKESCPIFPRPVMRIH
                     VGFHDPHGEPLDSFIAVREDIRARLVPAVRLALGL"
     gene            14097..15173
                     /gene="arsB"
                     /locus_tag="PKX01_08630"
     CDS             14097..15173
                     /gene="arsB"
                     /locus_tag="PKX01_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HMZ76229.1"
                     /translation="MSVIQCEVSTKAAAGAPMSVFERYLSVWVGLCIVAGIVAGQLAP
                     GLFRTIGRLEVAQVNLPVGLLIWVMIIPMLVKVDFGALHEVRQHVRGIGVTLFVNWFV
                     KPFSMAFLGWLFVRQLFAPLLPAEQLDSYIAGLILLAAAPCTAMVFVWSRLSDGDPLF
                     TLSQVALNDTIMVFAFAPLVAFLLGISSITVPWDTLLTSVVLYIVIPVILAQLWRRTL
                     LARGPAAFDAAMAKIGPWSIAALLATLVLLFAFQGEAILRQPLVIALLAVPILIQVFF
                     NSALAYGLNRLVGERHSVACPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLI
                     EVPVMLLVVKVVNASKGWYEARHT"
     gene            15184..15549
                     /gene="arsD"
                     /locus_tag="PKX01_08635"
     CDS             15184..15549
                     /gene="arsD"
                     /locus_tag="PKX01_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HMZ76230.1"
                     /translation="MKKLEVFDPAMCCSTGVCGVDVDPVLAQFAADLQWVEAHGIAVE
                     RHNLGQAPQAFAANPAVLKEMEAGMDRLPILVVDGHIVATGMYPSRVQLAQKLGIALT
                     TADKPHIKPGGCGCKPGSC"
     gene            15560..17311
                     /gene="arsA"
                     /locus_tag="PKX01_08640"
     CDS             15560..17311
                     /gene="arsA"
                     /locus_tag="PKX01_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HMZ76231.1"
                     /translation="MALPDSRTRYLFFTGKGGVGKTSLSCATGLALADAGKKVLIVST
                     DPASNLDEVLGAALGPLPTAIPGAPGLFALNIDPEAAARAYRERMVAPYRGILPAAAI
                     RSMEEQFSGACTVEIAAFDEFSKLLGDAAATTDFDHVIFDTAPTGHTLRLLTLPSAWS
                     GFIASSTGGASCLGPLAGLEQQKALYAATVARLSSAADTTVVLVSRAEAASLREAERT
                     RHELAGLGVSNLLLALNGVFRTERQDDAVATALSRRAAIALADMPAGLAALPATTIPF
                     LPRGTVGLAALRQMAHPESVAAPTAPDAAQTALPPGLAGLVETLAAAGHGVIMTMGKG
                     GVGKTTVAAAVAVALARRGHPVILSTTDPAAHVASTLDGQVPGLSVSRIDPAEEVARY
                     THEVLDKAGAQLDAGGRALLEEDLRSPCTEEIAVFRAFARTVEAGREGFVVLDTAPTG
                     HTLLLLDAAEAYHREVMRTQGDMPESVRELLPRLRDPDYTHVLIVTLAEATPVHEAER
                     LQHDLGRAGIAPFAWVINQSLLASGTRDPLLSQRGAWEIPFIRRVADELAPRCALIPW
                     LAEAPVGEAGLAQLLRT"
     assembly_gap    17544..17643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17703..18086)
                     /locus_tag="PKX01_08645"
     CDS             complement(17703..18086)
                     /locus_tag="PKX01_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ76232.1"
                     /translation="MGARHFALVAGLALIGPATAQDAARGAQLYEARCGGCHSPEANR
                     IGPRHAGVLGRRAGSLPDFAYSPALARSTVIWRNDTLERWLADPEALIPGQRMNYRVA
                     DAQDRRDLVAYLASLQVVAPDGGRR"
     gene            complement(18094..18672)
                     /locus_tag="PKX01_08650"
     CDS             complement(18094..18672)
                     /locus_tag="PKX01_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HMZ76233.1"
                     /translation="MSTLIQPARRGFLSTSGKTALSVAALGLLAGRSDLASAASGNAA
                     GDVAILNVALGLEHEAINAYQLGAGSGLLQKPVLDVAVLFQSHHKGHRDALIATIEKL
                     GGTPVKEKSLDEYARALKADTLKNQADVLSLAARLELGATNAYLGVIPSFKDAQLAQV
                     AGRLAADETMHWTALSSALGRSLPGGALSFGA"
     gene            complement(18753..21146)
                     /gene="ovoA"
                     /locus_tag="PKX01_08655"
     CDS             complement(18753..21146)
                     /gene="ovoA"
                     /locus_tag="PKX01_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HMZ76234.1"
                     /translation="MSRPLPDAADEPWRQNLRGEHDALLQGARPDWWWTGLPPTDCPG
                     RQPDGRLTALPAPNLATCTRDEVLAVFDNGWTLTEVLFAGLQGEEAFYRPPYHHLRHP
                     MIFYYCHPAALYINKLRVAGLIDAPLNPYFERVFETGVDEMRWDDMSKNAMLWPSFAE
                     AHSYRAEVYATVRRVIETHPDLATGHAPLGMDHPLWALFMGFDHERIHLETSSVLIRE
                     LPIALVQPPDVWPAVHSSAAAAATAEFPPRAGADYPVARWVDIPAASVRLGKPRDWPS
                     FGWDNEYGERRVAVQGFRVEDQLVSNGAFLEFIQAGGYLEPRWWSEAGWAWRSFRNSK
                     WPCFWVPDGPAGLHRYRLRTVFTIVPMPWAWPAEVNAHEAAAYCAWRSERDGHPCRLP
                     TEAEHNALRAPAWAAEDPAFRQDGAALGRDTGWNLQLAWGSPWPVDAGTPTAAGVRDV
                     FGNVWQWHGDDFNPLPGARVHPYYDDFSTPCYDGQHQMLLGGSWVACGDEASVWARFH
                     FRPHFFQHAGFRVVQAEHDGGVVRLGAADSGAQVYEDARMVDDYMVLHHARPEAQMPW
                     PAGPRGATGFPQRCADWLLKGAAQHASGTARALDIGCAVGGASFALARGFDAVLGVDL
                     SRAFIDAASTLQRDGRRDYVLRDEGELGDTLSAVIDPTLERSRVSFRQADACSLPAEL
                     ADFDAVLLANLLCRLPSPKSLLGRLGGPRGLVKVGGLVALFSPYSWLEQFTPQEAWLG
                     GFTADGRAVRSGDALKAFLTEEGFVLRREEDVPLVIREHARKYQYIVTHGMLWQRVR"
     gene            complement(21186..22343)
                     /gene="dinB"
                     /locus_tag="PKX01_08660"
     CDS             complement(21186..22343)
                     /gene="dinB"
                     /locus_tag="PKX01_08660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMZ76235.1"
                     /translation="MPPLPRSIAHLDMDAFYASVELLRAPELRGLPVVVGGRRALTAA
                     AGETHPRLRDYVGRGVITTATYEARALGVHSGMGLMKAAQLAPDAVLLPADFEAYRHY
                     SQRFKQAVRAIAPEVEDRGIDEIYLDLSALNPDGSVAGARALALRLQQAVRAATGLSC
                     SLGVAPNKLLAKLCSDLEKPAGITVVTLDDLPTRIGPLPARRINGIGPKAAARLEQLG
                     IDTIAQLAAADLDLLQHHFGERYGLWLQRAARGEDERPVVTRSEPKSLSRETTFERDL
                     HPRSDRQELSARFTRLCEQLAADLARKGYVGKTIGLKLRFDNFHTVTRDHTLAEPVGD
                     APTLRRVAGECARRVPLERRIRLLGVRVSGLVRRDALPPAESRPAQAELFA"
     gene            23130..23501
                     /locus_tag="PKX01_08665"
     CDS             23130..23501
                     /locus_tag="PKX01_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76236.1"
                     /translation="MTAAGKNRLPPQRVQDDIDAYLAIKVIPDWNPIKPEYAVDAITR
                     AHDALRAAQEAELHAQHTLDAARDALVSQQWDFHGIILGAKDQARAMFGPDSDHVAAL
                     GLKKKSDKKSPSRGPKPKPTE"
BASE COUNT         3627 a         8148 c         7993 g         3662 t
ORIGIN      
        1 cggcgagggc ggcttcggcg cgggctcgct cggactcggc gatgtggcgc tgggcctcgg
       61 cctgggcccg ttcggattcg gcgatgcggc gttgggcctc tgcctgctgg cgctgggttt
      121 cagcgatgcg ggcctcttcg gtggcgacgc gggcctgcca gacggccact ccggcggcga
      181 cacagatggc gcccacgccg gtccgcacga acaggcgggt gcggcgcaac tggcgcagat
      241 cggcgccgtc cagatcggtc gggctgacgc cgcgcagcgg cgcggcgacc cgcacgacgg
      301 cttcgcggaa gccgccgtgg gcgagggaca ggcccggcgt ctcccgcgcc cagcgcagat
      361 ccacgtagag cggttcgtcc gccagatggc cggcaaggca gtcgggcagc gcggtggtgc
      421 gcgtccagtc gaagtcgcgg cgtgcctcgt cccagacgat gtctccgtcg gtcaggacga
      481 tcagcaggcg ttccggactg cgttccgcca gccaccattc cacctccttg cggcaccagc
      541 gggagccggc gtagagcgga ttggcgaaga ccagcagcca gtcggacgcg gccaggtggc
      601 tgacgatgcc gggccacagg gccgggctgg cggtgaggtc ggtctggtcg cggaagatct
      661 tctgggcgcg caggcgcagc aggggtttgg cgagctgttc caggccgcgc tggaaggccg
      721 gcgccagggc gccgtccgcc gtgtggctgt aggaaatgaa ggcgtcgtga tggacaggca
      781 tggggccggc gaggcgaagg cgacggatct catcttcgca ccggcggccg gggcatggtc
      841 aaggggcagg cgtcagccgg gccagatgcc cgtgccggca aggaagggag atgcctcacc
      901 gtcctggctg tgcaggggca ggcgccgcct ggccgtccac agcgcgtgca cggtcagctg
      961 gagcacgcgc tgggcgccgg gatacgcatc cacggcgcga ccgtcgtgga ggatctgcgc
     1021 ggtggcggcg atccacacca ggctgccctc cgagaaattc acgaagagca gtccggcgcg
     1081 gggattcagg agcaggttgc cgagggtgtt gaagtactgg ttgccggcga agtcgggcac
     1141 ccacaggtgg tcgccgtcca cccccacgaa gccgggccgg ccgccccggt gggagacatc
     1201 cacgccgtgg gcggcgtgtt ccccccgtgc ggcatcggga tgggcggtgg cgatgaagaa
     1261 ggtgtcggcg tggcggatca ggtcggcggt cgcgccgtcg agtcgggcct ggacgcgggc
     1321 gggcgccggg gcagccgtct gcgccgcgtg ttcgacccgg cgggcgtgga tgtacttggg
     1381 gcagttgccg aagctctgtt cgacagccag ggtgaagccg tccgcgtcca gcgctgcgac
     1441 gcggccgttg gcccggttgc gacggcgggt gtggggctgc aggccaagca ggccgattgc
     1501 cgcaccgggg gcgagaccgg cggcggccgg gtcgtccgcc gccggaaggg ctccgatgcg
     1561 caggcgctcc ggtgtcgggc tggtgatgaa gccggggggg ccggcgatcg ccgtggccca
     1621 gggctggccg tccgcgtcca gggcgccgag cactgcgaaa ggcagttcgg cgaagaagct
     1681 gcggtgttgc tcgggcagca cctcacggat cacccgcggc cccgtttcgg ccaatcgcgc
     1741 ctgcgcgccg gcaagggcct gcagggcctc ctcgccggcg tggaagacgg atcgcggcgg
     1801 cgtctgcgcg gtcatgatgg ctcaggcccg gagtccggcc gcggtggccg gcatgggcac
     1861 gaaacccggc agggcttcga tgcgggcgag ccaggcgcgg atgtgggggt aggggtcgag
     1921 gctgacgttg ccttccggcg cgtgggcggt gtagctgtag agcgccacgt cggcgatggt
     1981 gggcgcgtcg cccgtcaggt aggcgcgcgt ggccagctcc gcatccagga tcttgaacag
     2041 ggcgtgggcg cgggcgatga cctcgtcggc gttgaagcgg gcgccgaaca cggtgatgag
     2101 gcgggccgcg cacggcccgt aggcgagctg gccggcggcc accgagagcc agcgctgtac
     2161 ctgcgcggcg gcggccggat cgcggggcag ccagcgaccg tcgtcataga cgctggccag
     2221 gtagaccagg atggcgttgg agtcggccac ggtcacgtcg ccatcctgga tcaccggcac
     2281 ctgcccgaag cagttcatgg ccaggtaggc cggggtcttc tgggcgccgc cggccaggtc
     2341 cacgtcgatg ggttcgaccg gcagaccgag cagggacagc atcagctcgg cccggtggct
     2401 gtggccggag gtggcgaagc ggtggaacct gatgggttgg gcgggggtgt tcatggcggt
     2461 cctttcggcg cgggggaggg atgtccggca gtgtggttta atccggcatg tgaataaatg
     2521 gcttaaaacg gatttaactg atccggaaat cggagtaatc catggataag ctgaaggcca
     2581 tcgggacctt cgtccgcatt gccgatgagg gcagcctgag cggcgccgca cgggtgctgg
     2641 acatgtccct gccggcggtg gtgcgggcgc tggcggcgct ggaggcggac ctgggggtgc
     2701 gcctgttcaa ccgcagtaca cggcgcatcg ccctcacgga ggaggggcgg cgttacctgg
     2761 acagttgccg caacctgctg gcggcactgg acgaggcgga atccgggctg cgggacgaag
     2821 ggggcgagcc gtcaggcgcg ttgacggtga cggcgccggt gcttttcggg caacttttcg
     2881 tggcaccggc catcacccgc ttcgtgcagc gcttcagccg cgtacattgc cgggtgctgc
     2941 tcttcgaccg gatcgtgaac ctgctggagg agggcgtgga tgtgggcatc cgcatcggcg
     3001 tgctggagga ctcgaccctc gtcgcccatc ccgtcggcca tgtgcggcgc atggtggtgg
     3061 cgagtccgga cttcctggct cgtcagggcg tgccggccca cccgcgggac ttgctgcggg
     3121 ccaattgcat cggcttttcg cccacgccgg gcgtgtggtg gcgtttcgag gagcaggggc
     3181 gcagtttcgg cgtgccggtg cgcggcaacc tggagttcaa ccacgtcgcc ccggccctgg
     3241 atgcctgcat cgccgggctt ggcttcggca tgttcatctc ctaccaggtg gcccctcatc
     3301 tggcggccgg gcggctggtt ccggtgctgg aagccttcga gcaggctccg cgcccggtca
     3361 gcgtggtggt gccccatgca cgcctcctac ccttgcgtgt gcgggccttt gtggactggg
     3421 tgcgggcgga tctctcagcg catctgcgcg acgcgccggg aacgcccggc agtacggcgt
     3481 gacgcggatt tcaggcccgc tgacatgcca atggtttcgt tgaaattcgc cggacggcgg
     3541 gataccatgc aacgaagggt attgtaaatc tttgtttccg cgaaaatacc ctttaaagtt
     3601 cggggttatt gctgaaatca tgaaaaaagc gtatctcgcc tccaccttcg tcctgatctc
     3661 cgcgcccagt cttgccgatg cgccggcgga tcccggcgag atccgcaatc tgcccctcag
     3721 cggcttcgtg gaccagttga gcctggaaca gctgggcaac ctggtggtga ccgacaccaa
     3781 gctcgcccag gcggcggata ccgtcacgca gcgcatcgtc gtgctgcgca acgaagacat
     3841 cgagcgccag cccgcgcact ccggcaatct ggcggagctc atgcgtccca cgtccggtca
     3901 gttcgtcaac gtgctgtccc gcaacgacgc caactggggc tcctacgcgg gtctcggtcc
     3961 caagtacaac agctatctgc tggacgggct gcccatcgat tccttcgtgg atgccatgag
     4021 cctcgacccc tccgccatcg agcgcgtcga ggtgcacaag gggccggcgt ccgtgctgta
     4081 ctccaattac ctgaccatgg atttcgtcgg caatgagacg cccctggccg ggaccaccaa
     4141 cttcgtgctg aagagccggg tcgatacgcc cctgacccgc gtcgctgccg gcgccgggtc
     4201 ctggggcacg gtgttcggca aggcctacgc gcaggggacg cagggcaatc tgggctacat
     4261 cgtcagtgcc aacctggagc attccgatta cacccagtac ggcacccgca attcctggct
     4321 gcagaccacc caggcgccgg cctaccagaa gaaccgattc ttcggcaatt tcagctacgc
     4381 cttcgaccgg cccgatcata cggtgacggt gttctatcaa cgcaccctgc accagggcac
     4441 gatgggccgt ccgaaccggg gcttcgacca cacctacgac accctcaact tcgcctacag
     4501 caacaccttc agcgacgact ggaacatcca gttcaagtat ggcgaacggc gctatgaccg
     4561 gcgcttcgcc aacgacaact acccgacgga cctgagcctc tccaaccacg aattcacccg
     4621 tcagaccatc cgccccgccg acctgaccct gagccatcgc cactggggcg agtcgctgct
     4681 gacggccggg gtggacggcc agtgggtgga ttatcagacc gacgtccagg atcccgtggg
     4741 ggccgtcacc ccgtcgaacc gcgcccaggc ccgctcgact ggttatttcg tgcaggagaa
     4801 gctggtgtgg gatgcctggg tgctgcgcgg aggagttcgg cacgacacta tccatcacga
     4861 ttacgacctg ctcggcggca tcacgcccgc cacgcgtagc gccgcgtgga gcaagaatct
     4921 gtggagcttc ggcacgcgct acaacgccga tcccggtctg gccttctacg gcaacgtggg
     4981 caccagtttc atggcgcccg cggccaagca gatcggtggc accgtggcgg tgcccaccgc
     5041 cagcggcgag ctgcccaatc ccggtctcaa gcccgaaacg ggcgtcggtc gtgacgtggg
     5101 cgtcaactgg cgcgccagcc aggcgctcac cctcgatctg cgggggttcc tgaacacgat
     5161 caacgacgcg atcgtcacca acatcgtcag cgcggcgccg tcccaggtac gttcagaaaa
     5221 cgcaggcaag gcccgcgcca tgggctacga ggtggatctc cgctacgcag cgtcgagcga
     5281 aaccagtgtg ttcggcaacc tcacccgtac ccacacccgg gtgaccgatc ccaccaacgc
     5341 ggatcatgac gggaccgcca tccccttcgc gccggacacc ctcgccaatg cgggggtgct
     5401 ggccagcctg ccgggcgaca tgagtgtcgc gacctattat cattgggtcg ggcgctacta
     5461 tgacagtacg tcccgcagcg cccgtctcgg tttcggccag tacggcgtgg tgaacgtccg
     5521 tgtgcagaag cccctgtacc gtgacgcccg cgcggcggtt catctgaccg tcgacctgaa
     5581 caacctgacc gaccggcacc atgccatgcc cttcgatttc cgcgatcccg gtttcaatgg
     5641 ctttgccggc gtcgacgtcc ggtattgagg cgtcgcggcc gcgggcgatg cgcgcgtccg
     5701 tccgccgccg gttccggctt gtcccgctgt tgcgccgcct gctggtgtgc gccgcagccc
     5761 tgcccctgat ggcgttttcg gcgccggggg agcttgaagc caagatcaag gcggcctacg
     5821 tcttccacgt catcaaattt gtggattggc ccgtgctgcc gacggacgcc ctgcgtctat
     5881 gcgtgagcgg cagcgatccg gtgggcgtcc tgctggggga actggccagc aagcaggtca
     5941 aggaccgtcc gctgcgcgtg gacatcgaca acggtttcga tccggcccag tgccaggtgc
     6001 tcttcgttgg ccggggcgag cggcgctggg ccgacctgca ggcgcgcctg cgcggcggga
     6061 gtgtgctcac catcagcgac caggacggtt tcgcccggat cggtggggtg gtgggctttt
     6121 attccgacgg tggccgcatc cgtcttgaga tcaatccgga cgccgcccgc aacgccaacc
     6181 tgcggatcag cgccaagctg ctcgaggtgg cgcgcaccgt gccgcaggac cgctgaaaga
     6241 tggtccgccc gcatcacgcc ccgcatcctg tcgcgccgtg cctggcggcg tgcgccggga
     6301 tctttccccc gaacccggca gatgaccatg cgcgcattgt ttgaaggcat gcagatcaag
     6361 gccaagctgg tgctgatcat cgtgatcacc agcgtggcgg cgctgctcat cgaagcggtc
     6421 ggcttcatcg cctacgagcg ggtgcgggtg accgacgaac tggtccgcga cctgtcgtcc
     6481 ctggcgcgca tcgtggccga ccgcagctcc gcggccctgc tgttctacga cgacaaggtc
     6541 gccatggaaa ccctgggcgc cctgcggatc aagcgggctg tcagcgccgc ctgcatctac
     6601 gacgtggggg gacgggtgtt tgcccgctac gagcggggtg aggaccgacc cttcagcttt
     6661 cccgccgatc ccaagttcga gagccgctcc tacaccgagg gcggttacct gcacctgatc
     6721 gagcccatcc ttgtcgaagg cacgccccag ggcgccgtgt tcatccgcgc cagcttccgc
     6781 gagctcgacc tgctgtggcg ggattacctg ctctttgcgg cgctcaccgc ctttgtcacg
     6841 tccatgatca ccctgctggt ggcgacgcgc tggcagcgca tcgtgtcgcg gcccatcgaa
     6901 cgtctcacgc gcacggtgca gatggtggcc atcagcaagg actacagcgt gcgggccacg
     6961 ctggacagcg acgacgagat cggcaccctg gtggaggtct tcaacggcat gctcgctgcc
     7021 atcgaggacc gggacaccgt gctgctggat gccaatcgcc ggctgtccga aaacgaggcc
     7081 cagctcaagt ccgtcaacga ggcgctggag cagcgggtgc gggagcgcac ggccgagctg
     7141 caggccctgt ccgactcggc cagcgtgggc atcgtgctgc tcaaggacgg cctcatcgtg
     7201 cgttccaacc ggcgtctcga cgagatgttc ggttacgcgc cgggcgagca gaccgggcgg
     7261 cccctgcggg atcgcctggc cgacaccgtg gacgaggagg ccgaggctct ggcggccgcc
     7321 cgtctggcgg ctggcgacac cgtgcgccgc gaactcatgg cgcggcacaa ggacggcagc
     7381 gcattctgga tccgcctgtc cgcccgcgtc atcgaccggg ccgatccggc gcggggcgcg
     7441 gtgtgcgtga tcgaggacat cacggccgag cgggacgcca tggacgagat gatcaaggcc
     7501 aagaccatgg ccgaggaagc gtcgcgcatg aagtccgact tcctcgccac catgagccac
     7561 gaaattcgca cgccgatgaa cgccatcatc ggcatgctct acctggccct caagaacgaa
     7621 ccgccggcgg ccctgcgcaa ctatctggcc aaggctcagg gcgccgccca ttccctcctc
     7681 gggatcatca acgacatcct ggatttttcc aagatcgagg ccggcaagct ggagatcgag
     7741 tccatcgagt tcggcctcga cacggtgctg gagcagctca aggattctgt cggcctgcag
     7801 gcggagcaga agggcctgga gttcctcatc cgctacgacg tcaatattcc ccccgcgctg
     7861 ctgggcgacc cgctccgcct cggccagatc atgctcaacc tgtgcagcaa tgctatcaag
     7921 ttcaccgagc agggcgaggt cgagctgggg ttccgctgcg tggcgatgac cgagacggat
     7981 ctggagctgc agttctcggt gcgcgacacc ggcatcggca tcgcaccgga cgtggcggag
     8041 cgcctgttcc agaagttcac ccaggccgat cagacgacca cccggcgttt cggcggcacc
     8101 gggctggggc tggccatcag ccgccgcctt gccgagctga tgggcgggcg cctgtggatc
     8161 gagcagtccg cgccaggcaa gggcaccacg gtgtgctgct cgatccacct gcagattgcc
     8221 cgggacgccc agacccatcg ccgccggctg gtggagcagg ccgggccgct ccttgacggc
     8281 gtgcgtgtcc tggtggtgga cgacaacgag gtgtcgcggg agatcatggc cgagatgctg
     8341 cgtttcttcc acctggacgt gagcgtggcg ggcagcggcg ccgaggcgct cgaacgcctg
     8401 caggtgccgg acgcggcgcc cgttgacctg gtgctgatgg actggcgcat gccgccgatg
     8461 aacggtgacg aggtcacccg gcgcatccac gcctcgccgc tcatccgcca caagcccaag
     8521 gtggtgatgg tcaccgccta cggtcgcgag gacgtcatca aatcagccga gcaggctgga
     8581 gtggccggct tcctggtcaa acccgtgtcc ccgtccaccc tcctcgacac catcctcagc
     8641 aatctcggcc gtgggcgtat cctcggcaag gagagcagca ggtccgaacg gggaggcgcc
     8701 tgcgacttcc gcggcgcgcg gctgcttctg gtggaagaca atgacatcaa ccgggaattc
     8761 gccaccgaac tgctgcgcag catgaacacc gaggtcgaat gtgcggtgaa tggcgaagag
     8821 gccgtggcca tggtccagcg tgccccctac gccgccgtgc tgatggacat ccacatgccg
     8881 gtgatggacg gcctggaagc ctgccggcgc atccgcgccc tgggccagca gcccggcggc
     8941 gaacacctgg gcgaactgcc catcatcgcc atgaccgccc ttgccatggc ccacgacgag
     9001 cagcgtagcc tggcggccgg catgaacgac cacatcacca agccggtgtc gccggagcgt
     9061 ctgcgcgcgg ccttgtcccg ctggctcaac attccgctgg gggagggcgg ggcgccaggg
     9121 cctgtcccgt ccagcgcgcc gggaagtgat ctgcgagccc tgcgcagcct cgatgccgag
     9181 cagggcatcc gccgcatcgg tggcgatgtc gaagcctatc gcaagcagct gcgacgcttc
     9241 cgacagcgct acgacacggc cgccgaacgc ctgcgcgacc tggtcgcaca ccatactccg
     9301 cgccaggccg aggagtattg ccacgcgctc aagggcgtct gtggcaatat cggtgccgac
     9361 gcgctgtttg cctgcgtgtc cgacatcgac aatctgctca agcaggagat ccggcccgat
     9421 gaggccagcc tcgcccgcat ggcggaactg ctcgccaccg tgatggccga catcgacagt
     9481 ctggctgcgc ccgagcaggc cggcgcgccc gcgggcggcg gtcttgctga taccgacgtg
     9541 ctggcccgcg tcgggcgtct gcgggggctg ctggagtccg acgtgggcgc agcccagagc
     9601 gtgctcgccg agttgctcgc cggcatcgcc ggttcgccgc tggaggccgc ggtccgcgag
     9661 gtggcggaac gggtcgatgt gttcgagatc gacgaagcgc ttggtctcct gcaggctctg
     9721 cagacgcaac tggaaggacg aaccggagcg acgccaccgt gacttcgcag gacgaagcca
     9781 agccgcgcat tctgattgtc gacgacatca acgacaatct gcacaccctg atgaacatcc
     9841 tgcggggtga gttcgccgtc gttgtcgcca ccaatggacc gaaggcgctc gaacttgccg
     9901 tccgcacgcc gccgccagac ctggtcctgc tcgacatccg catgcctggc atggacggct
     9961 acgaggtgct gcaccgcctc aagtccgatc cgggcaccgc cgacatcccg gtgatcttcg
    10021 tcaccggcct ctccgatgcc gccgacgagg ccgtcggcct gcgcctgggg gcggccgact
    10081 acgtcaccaa gcccatcaac cccgaactgc tcctggtgcg cgtgcgcaat cagctggagt
    10141 tgcgcaccca ccggcgcaag aaggctgtcg aaggcctcgg cgccgcggtc atggcgcgct
    10201 ccgacaagcc ggcgctgctg ctggtggacg atgtgcccga gaacctccac ggtctcgtcg
    10261 ccgcgctgca ggacgactac cgcatcatgg ttgcgcccac gggcgccaag gcgctggaac
    10321 tggtggccgg ggtgaatccg cccgacctga tcctccttga catcctgatg ccccagatgg
    10381 acggctacga ggtgtgccgc cgcatcaagg ccacgccggt gggcaaccgc atcccggtga
    10441 tcttcgtcac cgtggtggac agcgtggtgg acaaggtgca gggcttctcc atcggcgccg
    10501 ccgactacat caccaaaccc ttcgacatcg acgaggtgcg cgcccgcgtg cgcacccacc
    10561 tggacctcag ccgcctccag cgctccctcg agaagctggt cgtagaccgc atgggcgcgc
    10621 tgcaggaggc caacgatgcc ctcgccgaga gcaaggccaa gtaccagatc ctggccgagt
    10681 actcccccaa ctgggaatac tgggtcgcac ccactggcgg ctatctgtac gtctcgccgg
    10741 cctgccgcga cgtgtcgggc tacggggcgg tggatttcat cgccgacccg gatctgatgc
    10801 gcaagatgat ccatccggac gatctctaca tctgggatgc ccacaacgag cgctcgcccg
    10861 agtccggcgg ccatcgcctg cccaatttcc gcatcgctgc ccgcgacggc tccgaacgct
    10921 ggatcgagca cgtctgccag ccggtcatcg acgaccgcgg gcgtttcctc ggcacccgcg
    10981 gcaccaaccg agacgtgacc cagcaggtgg ccgccgagga acagctccac ctggccgcca
    11041 ccgtgttcgc caacacctcc gaggcggtga tgatcaccga cgcggacaac cgcgtcctca
    11101 ccgtcaatcc cgccttcgtc gagatcaccg gctattccca ggacgacacc gccgggcgcg
    11161 agcccctcgt gttgcgcgcc gacgtgatgg aggtggacgc ccatcgcgcc atgtgggccg
    11221 ccatccacgc caccggccgc tggcagggcg agatcaacgg gcactgccgc gacgggcgcg
    11281 acttcatcgg ctgggtcacc gtcagcaccg tctttaagga cgacggcagc gtcaaccggc
    11341 gcgtcgtgct gttctccgac atcaccgaaa agaaacgcgc cgacaacctc atctggaccc
    11401 aggccaacta cgaccacctc acccgcctgc ccaaccgccg cctcttcaac gaccgcatgg
    11461 agcgggagat caagaaggcc cagcgcaagg cctcggtgat cggcctgctg ttcatcgacc
    11521 tggaccgctt caaggaggtc aacgacaccc agggccacga ggtgggtgac ctgctgctgg
    11581 tggaagccgc cagccgcatc cgccactgtg tgcgcgacta cgacaccgtg gcccgcctgg
    11641 gcggcgacga attcaccgtc atcctgtcgg acatcaccga aggcaccgac atcggccgcg
    11701 tcgcccggag catcatcgac cgcctgtccg aagccttcgt cctcaacgcg caggaacatt
    11761 tcgtctccgc cagcatcggc gtcgccctct atccccgcga cggtgccacc atcagcgaac
    11821 tcatcaagca cgccgaccag gccatgtacc tggccaagga cgccgggcgc agccgcttcc
    11881 gcttcttcac ccgcgccctc caggagagcg ccgagcaccg catgcggctc gcgggcgacc
    11941 tgcgccgcgc cctcgaggcc ggtcagctcg aggtctatta ccagcccatc gtcgacctgg
    12001 acaccgggcg catcgccaag gccgaggccc tgttgcgctg gcgccacccg gcccacggct
    12061 tcgtcagccc cgccaccttc atccccatcg ccgaggacac cggcaccatc cacgagatcg
    12121 gtgaatgggt cttcgcccag gtcatccagc aggccagccg gtggcgcgag cgtctggcgc
    12181 cggaattcca ggtcagcctc aacatgtcgc cggtgcagtt caacgggcgc aatcccaccc
    12241 agtggctcga actgctgcag accacccagc tgcccggcag caacctggtc atcgaaatca
    12301 ccgagggtct gctgatgagc tccgatcccg gcatcaagga aacgcggctg ggcctgcgcg
    12361 acctgggggt cggcgtcgcc atcgacgact tcggcaccgg ctactcgtcg ctggcctacc
    12421 tcaagaagtt cgacatcgac gtcctcaaga tcgaccagtc cttcacccgc aacctggacc
    12481 ccaacagcgc cgacttcgcc ctgtgcgaag ccatcgtcgt catggcccac aagctcggcc
    12541 tgaaggtcat cgccgaaggt gtcgagaccg accagcaacg caacctgctg cgccagatcg
    12601 gttgcgacca cgcccagggc tacctgttct cccgtgcggt gccggcgggg cagttcgaag
    12661 ccctgtgcgc ccgctgagcc ggaccggcgc gggttgaaag cgcgattcat gacctgcttc
    12721 agcacgtcgc gacactttgc ccgcacccct gccgaggtgt tcgccgctgt ccgggatccg
    12781 gagcgcctcg cccgctggtg gggcccggac ggcttcacca atcggttcga gcgcttcgaa
    12841 ttccagcccg gcgggtgctg gtacttcacg atgatcgggc ccgacgggac ggcctatccc
    12901 aaccaggccg aattcacgcg catcgagccg gagtgctgcg tcgtgatccg ccacacctgc
    12961 gccccccatt tcaccctgac cctcaccttc gaacccgagg cgggcggcac gcgcctgcac
    13021 tgggcgcagg ctttcgacga tccggccgtc gccgaggcct tgcgcgccat cgtcgttccg
    13081 gcgaacgaac agaatctgga tcgcctggcg gcggtcctgg ggctcacgcc ggcctgacgc
    13141 gtcccgcggc gcagcacccg cctctcccgc agaaagtccg aacggcgcgc agtccgcgcg
    13201 tggatgcggt cgcaggtcgg attgacggcc tcgaatattt ccatataatt agaaatatgg
    13261 aactgcttga tgctgccgaa gtcctcgctg ccctggggca cgaatcccgt ctggccatct
    13321 ttcgcctgct cgtcgaggcg gggccggcgg gcctgaacgt gagcgccatt gccgagcgga
    13381 tggggggcgt gcccggcgcg accctgtcct tccatctgtc gcatctgagc cgcgtcgggc
    13441 tgatcaaggg cgagcgtcag agccgcttca tccattactc cgccgagtac gccacgatgg
    13501 acgagttgat cgccttcctg acgcgcaact gctgccaggg caaggcctgc ctgcccaaga
    13561 ccgcccagtg cgacacgacg gccaagcgtc gcgcggcccc tgccgacacc tgacatgccc
    13621 gcccgctgcg aggccctgca tcccgcgatc acccttttcg acggaacgcc ctgatgaacc
    13681 tgctcaaaac cgtcctcgtg ctgtgcaccg ggaattcctg ccgctcccag atgggcgagg
    13741 cgatcctcaa ctacgagttg gccggtgtcg tgcgcgccct ctctgccggc accgcgccgc
    13801 agcctaaggt ggcggatggc gcactcgaag ccctgcgcct cgccggcctg cccaccgagg
    13861 ggctccatcc caagaccgtc gaggcggtca tggaccagga catcgatctg gtggtcagcg
    13921 tctgcgacaa cgccaaggag agctgcccga tcttcccgcg cccggtgatg cgcatccacg
    13981 tgggcttcca tgatccccac ggggagccgc tggacagttt catcgccgtg cgcgaagaca
    14041 tccgcgcccg tctggtaccg gccgtccgtc tggctttggg tctgtgaggg gcggccatgt
    14101 cagtcattca atgtgaagtg agtaccaagg ccgccgccgg cgcaccgatg agcgtcttcg
    14161 agcgttatct gagcgtgtgg gtgggcctgt gcatcgtcgc cggcatcgtc gccggccagc
    14221 tcgcccccgg gctgttccgg accatcggcc gcctggaggt ggcgcaggtc aatctgcccg
    14281 tcggcctgct catctgggtg atgatcatcc ccatgctggt caaggtggat ttcggcgcct
    14341 tgcatgaggt ccgtcagcac gtccgcggca tcggcgtgac gctgttcgtc aactggttcg
    14401 tcaagccctt ctccatggcc ttcctgggct ggctcttcgt gcgtcagctg tttgcgccgc
    14461 tgctgccggc agagcagttg gacagctata tcgccggcct catcctgctg gcggcggcgc
    14521 cgtgcacggc catggtcttc gtgtggagcc ggctgtccga cggcgacccc ctgttcaccc
    14581 tgtcccaggt ggccctcaac gacaccatca tggtgttcgc cttcgcgccc ctggtggcct
    14641 tcctgctggg catctcgtcc atcacggtgc cctgggacac gctgctcacc tcggtggtcc
    14701 tctacatcgt gatccccgtc atcctcgccc agctgtggcg acggaccctg ctggcccgcg
    14761 ggccggcggc cttcgacgcg gcgatggcga agatcggccc ctggtccatc gccgcgctgc
    14821 tggccaccct ggtgctgctc ttcgccttcc agggcgaggc catcctgcgt cagccgctgg
    14881 tcatcgccct gctggcggtg cccatcctga tccaggtgtt cttcaactcg gcgctggcct
    14941 acgggctcaa ccggctggtg ggcgagcggc acagcgtcgc ctgcccgtcg gccctcatcg
    15001 gcgcgtcgaa cttcttcgaa ctggccgtgg ccgccgccat cagcctgttc ggcttcgaat
    15061 ccggtgcggc gcttgcgacg gtggtgggcg tgctcatcga agtgccggtc atgctgctgg
    15121 tggtcaaggt ggtcaatgct tccaagggct ggtacgaagc ccgtcacacg taaggagcgc
    15181 atcatgaaga aactcgaagt cttcgatccc gccatgtgct gttccacggg cgtctgcggt
    15241 gtggacgtgg acccggtgct ggcccagttt gctgccgacc tgcagtgggt cgaagcccac
    15301 ggcatcgctg tcgagcgtca caacctgggt caggcgcccc aggccttcgc ggccaatccc
    15361 gcggtgctca aggagatgga agccggcatg gaccgcctgc ccatcctcgt cgtggatggc
    15421 cacatcgtcg ccaccggcat gtacccgtcc cgggtgcaac tggcccagaa gctgggcatc
    15481 gccctgacga ccgccgacaa gccccacatc aagcccggcg gctgcggctg caagccgggc
    15541 tcctgctgag ggcgccgcca tggccctccc cgattcgcgc acccgctacc tgttcttcac
    15601 cggcaagggc ggcgttggca agacgtccct gtcctgcgcc accggcctgg cgctggcgga
    15661 cgccggcaag aaggtgctga tcgtcagcac cgacccggcg tccaacctgg acgaggtgct
    15721 gggcgcggcc ctgggcccgc tgcccacggc gattcccggc gcgccggggc tgttcgccct
    15781 caacatcgac ccggaggccg ccgcccgcgc ctaccgggag cgcatggtcg cgccctaccg
    15841 cggcatcctg ccggcggcgg ccatccgcag catggaggag cagttctccg gcgcctgcac
    15901 cgtggagatc gccgccttcg acgaattctc caagctgctg ggcgacgcgg ccgccaccac
    15961 ggatttcgac cacgtcatct tcgacaccgc cccgaccggc cacaccctgc gtctgctcac
    16021 cctgccgtcg gcgtggagcg ggttcatcgc gtcgtccacg ggcggcgcct cctgcctggg
    16081 gccgctggcc ggccttgaac agcagaaggc cctctacgcg gccaccgtgg cgcgcctctc
    16141 cagcgcggcc gacaccaccg tcgtcctggt cagccgcgcc gaggccgcct ccctgcgcga
    16201 agccgagcgc acgcgccacg agctggcggg gctcggcgtc tcgaatctgc tgctggccct
    16261 caacggcgtg ttccgcaccg aacgccagga cgacgccgtc gccacggccc tgtcccgccg
    16321 tgccgccatc gccctggccg acatgcccgc cggtctggcc gcgctgcccg ccaccacgat
    16381 ccccttcctg ccccgcggca cggtgggcct ggcggccctg cggcagatgg cccatcccga
    16441 gtccgtcgcc gcccccaccg cgcccgacgc ggcgcagacg gcgctgccgc ccggcctcgc
    16501 cggcctggtg gagaccctcg ccgccgccgg tcacggcgtc atcatgacca tgggcaaggg
    16561 cggcgtcggc aagaccacgg tggcggcggc cgtggcggtg gccctggccc gtcgcggcca
    16621 tccggtgatc ctgtccacca ccgatcccgc cgcccacgtg gccagcaccc tcgacggcca
    16681 ggtgcccggc ctgagcgtca gccgcatcga ccccgccgag gaagtggccc gctacaccca
    16741 tgaagtgctg gacaaggccg gcgcccagct ggatgccggc ggacgggccc tgctggaaga
    16801 agatctgcgt tcgccctgca ccgaggagat cgccgtgttc cgcgccttcg cgcgcacggt
    16861 ggaggcgggc cgcgagggct tcgtggtgct ggacaccgcc cccaccggcc acaccctgct
    16921 gttgctggat gcggccgagg cctaccatcg ggaagtgatg cgcacccagg gcgacatgcc
    16981 cgagtcggtg cgcgagctgc tgccgcggct gcgcgatccg gattacaccc acgtgctcat
    17041 cgtgaccctc gccgaagcca ccccggtgca cgaggcggag cgcctccagc acgacctggg
    17101 gcgggccggc atcgccccgt tcgcgtgggt catcaaccag tccctgctgg cgagcggcac
    17161 gcgcgacccg ctgctgtccc agcgcggcgc ctgggagatc cccttcatcc gccgcgtggc
    17221 ggacgaactc gcgccgcgct gtgcgctgat cccctggctg gcggaggcgc ccgtgggcga
    17281 ggccggtctg gcccagctcc tgcgcacctg agccccgcgc gtccgtcgcg cgggcagggc
    17341 ccggccatgg tgagcgacgg cgatgacctg tagccgatct gccaaatccc tgcgcgcggg
    17401 tctacccggt ctgcggcggc gccggcagag tcttcgtccc cttcgtcgga cgctcggcac
    17461 cgcgcccgcg ccgtgtccgc gccatcgaca cggcccggcg cgatgcccgc gcatgtcacg
    17521 gccgactccg ccgcgggatt gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnncacggcc cggcgcgatg cccgcgcatg tcacggccga ctccgccgcg ggattgccgg
    17701 cgtcagcggc gtccgccgtc cggcgcaacc acttgcaggc tcgccagata ggccaccaga
    17761 tcgcgccggt cctgggcgtc ggcgacccgg tagttcatgc gctggcccgg aatcagggct
    17821 tccgggtcgg cgagccagcg ttccagggtg tcgttgcgcc agatcacggt ggaccgggcc
    17881 agggccggcg agtaggcgaa gtccggcagg ctgccggcac ggcgtccgag gacaccggcg
    17941 tggcgcggcc cgatgcggtt cgcctccggc gagtggcagc cgccgcagcg cgcctcgtag
    18001 agctgcgcgc cgcgggcggc atcctgggcg gtggccgggc cgatcagggc gagcccggcg
    18061 accagggcga agtgccgggc gcccacgacc tgctcaggcg ccgaagctga gggcgccgcc
    18121 cggcagactg cggccgaggg cgctgctcag ggcggtccag tgcatggtct cgtcggcggc
    18181 gaggcggccg gccacctggg cgagctgggc atccttgaag gacgggatca cgcccagata
    18241 ggcgttggtg gcgcccagtt ccaggcgcgc ggcgagggac agcacatcgg cctggttctt
    18301 gagcgtgtcc gccttgaggg cgcgggcgta ctcgtccagg gacttctcct tgaccggcgt
    18361 gccgccgagc ttctcgatgg tggcgataag cgcatcccgg tggcccttgt ggtgggactg
    18421 gaacagcacc gccacgtcca gcaccggctt ctgcagcagg ccgctgcccg cgccgagctg
    18481 gtaggcgttg atggcttcgt gttccaggcc gagggccacg ttgaggatgg ccacgtcgcc
    18541 ggcggcattg ccgctggcgg ccgacgccag atcgctgcgg ccggccagca ggccgagggc
    18601 ggccacggac agggcggtct tgccggaggt ggacagaaag ccgcggcggg cgggctggat
    18661 gagggtgctc atgatcggat ctcctgaagg ttgcgggggg gtgtcttcca cccccttcta
    18721 cgcagccggc cggcatccgg atgcagcgcc cctcagcgca cccgctgcca gagcatcccg
    18781 tgggtcacga tgtactgata cttgcgggcg tgctcgcgga tcaccagggg cacgtcctcc
    18841 tcgcggcgca gcacaaagcc ttcttcggtg aggaaggcct tcagcgcgtc gccgctgcgc
    18901 accgcgcggc cgtcggccgt gaaacccccg agccacgcct cctgcggcgt gaattgctcc
    18961 agccacgaat agggcgagaa cagcgccacc agcccgccca ccttgaccag gccccgcggc
    19021 ccgcccaggc gaccgagcag cgacttggga ctgggcaggc gacacagcag attggccagc
    19081 agcaccgcgt cgaagtccgc cagctcggcg ggcagggaac acgcatcggc ctgacggaag
    19141 ctcacccggc tgcgttcgag ggtcgggtcg atgaccgcgc tcagggtgtc gcccaattcc
    19201 ccttcgtcgc gcaggacata gtcacgccgg ccatcccgct gcagggtgct ggccgcgtcg
    19261 atgaaggcgc gcgacagatc cacccccagc accgcgtcaa aaccccgcgc cagcgcgaag
    19321 ctcgccccgc ccaccgcgca gccgatgtcc agggcgcggg cggtgccgct ggcgtgctgg
    19381 gcggcgccct tcagcagcca gtccgcacag cgttgcggaa agcccgtggc cccccgcggg
    19441 ccggccggcc agggcatctg ggcctccggg cgggcgtggt ggaggaccat gtagtcgtcc
    19501 accatgcgcg cgtcctcgta tacctgggcg ccgctgtccg ccgcccccag gcgcaccacg
    19561 ccgccgtcgt gctccgcctg caccacgcgg aagccggcgt gctggaagaa gtggggacgg
    19621 aagtggaagc gcgcccacac gctggcctcg tcgccgcagg ccacccacga gccgcccagc
    19681 aacatctggt gctggccgtc gtagcagggc gtggagaagt cgtcgtaata gggatggacg
    19741 cgggcaccgg gcagggggtt gaaatcgtcc ccgtgccact gccacacgtt gccgaacacg
    19801 tcgcgcacgc cggcagcggt gggcgtgccc gcatccaccg gccacggcga gccccaggcg
    19861 agctgcaggt tccagccggt gtcgcggccg agggcggcgc cgtcctggcg gaaggccggg
    19921 tcctcggccg cccaggccgg cgcgcgcagg gcgttgtgct ccgcctcggt gggcaggcga
    19981 caggggtggc cgtcgcgctc gctgcgccag gcgcaatagg cggcggcctc gtgggcattc
    20041 acctcggccg gccacgccca gggcatgggc acgatcgtga agaccgtgcg tagacggtag
    20101 cgatgcaggc cggcggggcc gtccggcacc cagaagcacg gccacttgct gttgcggaag
    20161 ctgcgccagg cccagccggc ctcgctccac cagcgcggct ccagatagcc cccggcctgg
    20221 atgaattcca ggaaggcgcc gttgctgacg agctggtcct ccacgcgaaa gccctgcacc
    20281 gcgacgcggc gctcgccgta ttcgttgtcc cagccgaagc tgggccagtc gcggggcttg
    20341 cccaggcgca cgctggcggc ggggatgtcc acccagcggg cgacgggata gtccgcgccc
    20401 gcgcggggcg gaaactcggc cgtggccgcc gctgcggcgc tggaatgcac ggcgggccac
    20461 acgtccggcg gctgcaccag ggcgatgggc agctcgcgga tcagcaccga cgaggtctcc
    20521 aggtggatgc gctcgtggtc gaagcccatg aacagcgccc acaggggatg atccatgccc
    20581 agcggcgcgt ggccggtggc cagatccggg tgggtctcga tgacgcggcg cacggtggcg
    20641 tacacctccg cccggtagct gtgggcttcg gcaaaggacg gccacagcat ggcgttcttg
    20701 gacatgtcgt cccagcgcat ctcgtccaca ccggtctcga agacgcgctc gaagtagggg
    20761 ttgaggggcg cgtcgatgag gcccgccacc cgcagcttgt tgatgtacag cgccgccggg
    20821 tggcagtagt agaagatcat cgggtggcgc aggtgatgat aaggcgggcg gtagaacgcc
    20881 tcttcgccct gcaggcccgc aaacagtact tcggtgagcg tccagccgtt gtcgaagacc
    20941 gccagcacct cgtcccgcgt acaggtggcc aggttgggcg ccggcagcgc ggtgaggcgg
    21001 ccgtcgggct ggcggccggg gcaatcggtg ggcggcaggc ccgtccacca ccagtcgggc
    21061 cgggcgccct gcagcagggc gtcgtgttcg ccgcgcaggt tctggcgcca gggttcgtcg
    21121 gcagcgtcgg ggagggggcg ggacatggac ttctccggag aagggctcgg gtcggggtga
    21181 tgggctcagg caaacagctc ggcctgggcc ggtcgggatt cggcgggcgg cagcgcatcg
    21241 cggcgcacca ggccggagac gcgcacgccc agcagacgga tgcgtcgctc caggggcacg
    21301 cgcctggcgc attcgccggc cacccggcgc agggtcggcg cgtcgccgac gggctccgcc
    21361 agggtgtggt cgcgggtgac ggtgtggaag ttgtcgaagc gcagcttgag gccgatggtc
    21421 ttgcccacgt agcctttgcg cgccaggtct gccgccagct gttcgcacag acgggtgaag
    21481 cgggccgaga gttcctgccg gtcgctgcgg ggatgcagat cccgctcgaa ggtggtctcc
    21541 cgggacagac tcttgggttc gctgcgggtg accaccggac gctcgtcctc accccgcgct
    21601 gcccgctgca accacaggcc gtagcgctcg ccgaagtgat gctggagcag gtccagatcg
    21661 gcggcggcaa gctgggcgat ggtgtcgatg cccagctgct ccaggcgggc cgccgccttg
    21721 ggtccgatgc cattgatacg tcgcgccggc agggggccga tgcgggtggg caggtcgtcc
    21781 agggtcacca ccgtgatgcc ggcgggcttc tccaggtcgg aacacagctt ggccagcagc
    21841 ttgttgggcg ccacgcccag ggagcacgac aggccggtgg ctgcccgcac cgcctgctgc
    21901 aggcgcaggg ccagggcgcg ggcgcctgcc acgctgccgt ccgggttgag ggcagagagg
    21961 tccaggtaga tctcgtcgat gccccggtct tccacctccg gcgcaatcgc ccgcaccgcc
    22021 tgcttgaagc gttgcgaata atgccggtag gcctcgaaat ccgcgggcag cagcactgcg
    22081 tccggcgcca gttgcgccgc cttcatcagc cccattccgg aatgcacgcc cagcgcccgt
    22141 gcctcgtagg tggcggtggt gatcacgccg cgccccacgt aatcccgcag gcgtgggtgc
    22201 gtctcccccg cggcggccgt cagcgcgcgc cgcccgccca ccaccaccgg caggccccgc
    22261 aattccggcg cgcgcaacaa ttccacggac gcataaaagg cgtccatgtc caggtgggcg
    22321 atgctgcggg gcaggggagg catgacggcg atagacggca gagggcctta ttgtgcctga
    22381 aggccgccga cgcagtgatg acgcggccga ccgcagcgct ggggcgggga tgtcgtctgc
    22441 gccggccggt accgggcggg gtcgggccgg cgccacccgg gcgctggggc cggagcaggg
    22501 gccgacggaa gccaccaggc cgcgtcggcc cccgcagtgc aggcggacac ctccaccctt
    22561 gccgtggcgc cgaccgcagc gtctgcgcac ggcgcctcag atcatgggga cctgcgctca
    22621 gatgctgtcg cgggcagcga agatcatgat cacgcgtgct cggacagtgt gcacgggtgc
    22681 acaccgtctg ttgacccgtg cacagccttt gtcgaccggt cgacagtctt gtaagcgccg
    22741 tccacatcaa ggatcgatag ttctttccag tgttctgagg cctcaaacaa aggtaaaggg
    22801 ctatcaacag tgtgtgtgtc cacgtccaca tcgtctgtcg acgcctgcgc agacactgtc
    22861 gggctgtgca cagtgtgttg gacgcccatc cttcacccat cgccgccctc aacagacagc
    22921 gtgcgcaggc acgcagcgtc ttcgcacatc ggcacaggcc tgtccggacg gccagacagt
    22981 attgtgctgc gtgatgggcg ccattaaaaa ccatgagatc ggcggtgtct catggcatgg
    23041 cttgcccgat tgtcatcttc gcggtattgg atatgattgc gctgtcattt atgacagaga
    23101 agactttcac cccaccgaag gagacatcca tgactgctgc cggcaaaaac cgcctgcccc
    23161 cgcaacgggt ccaggacgac atcgacgcct acctcgccat caaggtcatc cccgactgga
    23221 accccatcaa acccgaatac gccgtcgacg ccatcacccg cgcccacgac gccctgcgcg
    23281 ccgcccagga agccgaactc cacgcccagc acaccctcga cgccgcccgc gacgccctgg
    23341 tcagccagca atgggacttc cacggcatca tcctcggcgc caaagaccag gcccgcgcaa
    23401 tgttcggacc ggattccgat cacgtggccg cgctgggctt gaagaagaaa tcggacaaga
    23461 agtcaccgag ccgagggccg aagccgaaac cgacggagtg aggaggggtt tgggctgaca
    23521 tgaacggaga
//
LOCUS       DAOQPA010000049        23353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1042_length_23353_cov_17.007039, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000049 DAOQPA010000000
VERSION     DAOQPA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23353
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1042_length_23353_cov_17.007039"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PKX01_08670"
     CDS             complement(<1..112)
                     /locus_tag="PKX01_08670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HMZ76237.1"
                     /translation="MPLVSMRQLLDHAAENGYGLPAFNVNNLEQVQAIMEA"
     gene            complement(227..1669)
                     /gene="pyk"
                     /locus_tag="PKX01_08675"
     CDS             complement(227..1669)
                     /gene="pyk"
                     /locus_tag="PKX01_08675"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ76238.1"
                     /translation="MSRHTKIVATLGPASSDPATLERLVQTGVDVVRLNFSHGTAEDH
                     IRRATQIRDAAQLVGRPVGILADMQGPKIRIGKFEQGKIALDKDAPFILDARCELGNA
                     QRVGLDYKDLPRDVKPGDVLLLDDGRLKLGVERVLGHEIHTRVKVGGELSNNKGINRQ
                     GGGLTAPALTAKDMEDIKTAAQIGVDFVAISFPKGAADMYMARQLLRAAGSSAVLIAK
                     IERTEAVANLEEILDASDGIMVARGDLAVEVGDAAVPALQKRMIRLARERNKLAITAT
                     QMMESMIHSPVPTRAEVSDVANAVLDGTDAVMLSAETASGKFPVEVVEAMARICLEAE
                     KSAEVTMDRDFLDRIFTRIDQSIAMAAMWTAYHLKVKAIASLTQTGSTALWMSRLNSG
                     VPIYALTPELTSRNQMTLYREVYPLLMSQMHADRDMLLWESEQILLEQGVVDYGDLIV
                     LTIGEPIGASGGTNTMKIVRVGDTPRPGPV"
     gene            complement(1732..3696)
                     /locus_tag="PKX01_08680"
     CDS             complement(1732..3696)
                     /locus_tag="PKX01_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HMZ76239.1"
                     /translation="MPFSAPRPFSWKQASVVVPTLIALLLALVGVSQGLHRLTQEKEQ
                     DQALDFAYRSAEMHFQIGERFKAYRQVLRGARALFAASERVSRSEWKAYARELRLDTE
                     FPGIQGVGFALSVAAAHQGAHEQAVREEGFADYRISPPGERAEYAPIVYLEPFDWRNR
                     RAFGYDMLSEPVRRTAMLRTRSLGLATLSGKVRLVQETETDPQAGVLLYLPVFRNGTS
                     PATPAQREEAFVGWVYSPIRMKDLITGTLGNTPDRIRLRIFDGESRTAEALLFDSHPE
                     LAQVGRFQATYVLELDGRTWTLAFESTPAFSMGKTVDTVEATSVVLIGALIVLLTASF
                     AGARQRALDLDRIGASLRASEARYSTLVNLSRDGIAAVDGGLRFTFVNPRLAELLGHP
                     ADALLGRRLDSLWPAPDEPRSRTLIRRLHRGEGATYEQELIGARGAAVTTQISDAPLL
                     GRNGALLSVIVTVTDISERKESEQRIRYLATHDALTGLANRTMFLELMDASLRLTSRR
                     GTRLAVFFLDLDGFKAVNDRLGHHAGDQLLVATARRIRACLRASDHLARQGGDEFLAL
                     IHDVAGADEAVAVARKIHQSLEEPFDLPEGRALISVSIGIALFPVDGTDIDTLTRQAD
                     SAMYQAKAGGRNGIRLAGEPVPPEAPQQGS"
     gene            3923..4981
                     /gene="rfbB"
                     /locus_tag="PKX01_08685"
     CDS             3923..4981
                     /gene="rfbB"
                     /locus_tag="PKX01_08685"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HMZ76240.1"
                     /translation="MILVTGGAGFIGANFVLDWLATCDEPVVNLDALTYAGNLENLAS
                     LKGDPRHVFVQGDICDRPLIDRLLAEHQPRAIVHFAAESHVDRSIHGPGEFMRTNVTG
                     TFTLLEAARAHWNGLEGAAKADFRFHHVSTDEVYGSLGPTDPAFTETKTYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVTTSNCSNNYGPYHFPEKLIPLMIANALAGKPLPVYGDG
                     MQIRDWLYVTDHCSAIRAILEKGQPGEVYNVGGWNEKPNIEIVRTLCALLDELKPDPA
                     GSYARLITYVTDRPGHDRRYAIDARKIERELGWRPAETFDTGIRKTVEWYLAHQDWVA
                     HVQSGAYRDWVEQNYAAR"
     gene            5041..5946
                     /gene="rfbD"
                     /locus_tag="PKX01_08690"
     CDS             5041..5946
                     /gene="rfbD"
                     /locus_tag="PKX01_08690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HMZ76241.1"
                     /translation="MKILLLGKNGQVGWELQRSLAPLGDLVALDRHGDGGWIGDLADP
                     DGLAATVRAVAPDLIVNAAAHTAVDKAESEPDLAFTINALAPGALARAAATCGALLVH
                     YSTDYVFDGRGTAPWAEGAPTGPLSVYGRTKLEGETLIRDSGCRHLIFRTSWVYAARG
                     GNFARTMLRLAVERDALSVVDDQHGAPTGAELLADVTAHAARMTFARPELAGTYHLAA
                     AGETTWHGYARHVVEFARARGVPIRVAPDAIRPVPTSAFPTPAQRPANSRLDTRKLRD
                     AFGLVLPDWQQGVERMLTEILPNSN"
     gene            5958..6839
                     /gene="rfbA"
                     /locus_tag="PKX01_08695"
     CDS             5958..6839
                     /gene="rfbA"
                     /locus_tag="PKX01_08695"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMZ76242.1"
                     /translation="MQRKGIILAGGSGTRLHPATLAVSKQLIPVYDKPMIYYPLSTLM
                     LAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYAVQPSPDGLAQAFIIGADFVGNA
                     PSALVLGDNLFYGHDFSSNLRTASARTDGATVFAYPVHDPERYGVVEFDAQGHAVSLE
                     EKPRQPKSRYAVTGLYFYDQRVVDVARAIQPSPRGELEITDVNRQYLEWGALDVQVMG
                     RGHAWLDTGTHESLLEASLFIQTLEKRQGLKIACPEEIAWRAGWIDAARLQQLAAPLA
                     KNGYGQYLLRILDEKVF"
     gene            6852..7406
                     /gene="rfbC"
                     /locus_tag="PKX01_08700"
     CDS             6852..7406
                     /gene="rfbC"
                     /locus_tag="PKX01_08700"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMZ76243.1"
                     /translation="MNLIQTAIPDLLILEPKVFGDARGFFMESYNARTFRELTGLDVD
                     FVQDNHSRSAKGVLRGLHYQIQQAQGKLVRVVRGSVFDVAVDLRKASPTFGLWAGVEL
                     SEENQRQVWIPPGFAHGFLVTSDSADFLYKTTDYYAPQHERCLLWNDPVVGVAWPLAQ
                     IDGAPQLSAKDVAGKGLADCEVFA"
     gene            7507..8613
                     /gene="wecB"
                     /locus_tag="PKX01_08705"
     CDS             7507..8613
                     /gene="wecB"
                     /locus_tag="PKX01_08705"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMZ76244.1"
                     /translation="MSQSLIYLVAGARPNFMKIAPIVRALQARADRFTFKIIHTGQHY
                     DREMSDVFFDELGIPKPDYHLEAGGGSHATQTAKIMVAFEEICQKVRPDCVLVVGDVN
                     STLACAIDAKKLQIPVAHVEAGLRSGDMRMPEEINRLVTDSISDWFFCTEPAGVDNLL
                     KEGKQPEALFHVGHVMVDNLLYQRDQLNASDKSGMESEALKRRYPKYGVVTLHRPSNV
                     DDETHLRGIAGALKQIAADLPLIFPVHPRTRGNLEKFGLDLGPNVVLTKPQSYMEFLN
                     LWKDAALIMTDSGGLQEETTALGIPCLTLRENTERPVTIDEGSNVLVGPDPARILEEA
                     AKVLAGQGKQGRRPALWDGKAAERIVADLDRLLP"
     gene            complement(8638..9378)
                     /locus_tag="PKX01_08710"
     CDS             complement(8638..9378)
                     /locus_tag="PKX01_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76245.1"
                     /translation="MLAAIEALFLPALRAALGPDASLRSGPAGAPAGPSGQVSLWAEK
                     LEWDGAEGEDPTRDPVCAGWQGEVSPDPASPLDFPLPEAARGEVAEVQSPPGRILPPG
                     DAWQIDGRTLRFFSAPGGPVRVSTRTVPVRGYRERRFGHVALSLRAWAHDGPAVDSLL
                     ATGLAALLAAAENFNVVELAGAAPSLSVRLTRPRLSVVALEQDADGPWTSGRVRCVLR
                     GEVELALRLGEAAPEGRIRDVTVTLRRP"
     gene            complement(9388..10683)
                     /locus_tag="PKX01_08715"
     CDS             complement(9388..10683)
                     /locus_tag="PKX01_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76246.1"
                     /translation="MADPVQLLGPDGAPAAGRSFSAAEGIRLRVTLPAGRRLAEARLS
                     LASADAAEQAVLYPPLQTDAGAADGQTEASWRWAAIDWRHERALTGVIIDARDASTPP
                     RTCAARVKLYSRGHWLPLTPLDALPTGAVQRFPPLAASRLMAEMLVEGDVGGSRSGVP
                     IPGALTGKRIGAILTGQACHVSLAMEDLPPFFAPEGPLPTQAQPVEGLTRILNRYLSD
                     HPGTTEIPLLLRAAGDEAVRVGPFAATLEAPAQADDAAAPPVDPHQARPGEQPWFPPG
                     RAPLQAARLIDPQHQAAQSFAPLPADRSLSSTALRLRPLADAFVGNVRLHALRGDQTV
                     EPPLAEWPLSFGPDSAQSADAVWLRFRLPETVAPAGPWRLTCQATSGALLWYVDDSPR
                     PAPLQSGLARTGQGPWLPLDGPGEAPWLQVEVGLLDTAP"
     gene            complement(10691..10888)
                     /locus_tag="PKX01_08720"
     CDS             complement(10691..10888)
                     /locus_tag="PKX01_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76247.1"
                     /translation="MKNELTARLAQLHEEYDAGTRVMADLQKKQDELRTTLLRISGAI
                     QVLEELLGESAEQPAKPPPAA"
     gene            complement(10911..12311)
                     /locus_tag="PKX01_08725"
     CDS             complement(10911..12311)
                     /locus_tag="PKX01_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76248.1"
                     /translation="MPTRPDPYVKRAAGDIIRADDWNEAQVRAAEALAAHDHRAGQGQ
                     PVPREGIAEKAIDGSRIDPAASVTLQALDVRGELKVNARALLAEIDGLIAGLKNALNR
                     NGDVLRGGLAITERLAVGSEKLASARLQLGELSALDIGARNNAWTNLGWNAYFDGAWK
                     RLDPNKPGANLHLSGDGAGQEFRFLRVEADGSALRNVAVIGSKVSAILESNLGLGTAA
                     PNARLDVRGAIHAGGSDLYFTETGHRHTGFGNAAGYAAIENDGGDYNALMILGRSISQ
                     NPLSRAVKMYDNVTISGNLGTHGFAPSPRTGGWGGGIHTWDVEAEGTMWSRNGYQSGN
                     RDLAENFDAVEALAPGDVVSLDPDRDGIRRSRQARDPLVLGVVSTRPGVLLGADADRQ
                     PPFAGEVPVALSGRVPCQVTDENGPIRRGDLLTAASRPGHAMRAGDAPPGTLIGKALA
                     DFDGGTGTIDVFVFVR"
     gene            complement(12325..13725)
                     /locus_tag="PKX01_08730"
     CDS             complement(12325..13725)
                     /locus_tag="PKX01_08730"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMZ76249.1"
                     /translation="MADTPTPYVRKDPGDIMLAADWNQMQVRARQEIQAHDHSGDPFQ
                     QIPREGIREKAIDGSRIDPDAKVALKGLTVSGPLKITGTSQLADIKAALASFTGARLT
                     TAGDLGVGTDAPENAEGWSRVVDILGGAHAKLAVRAGDVDARVMAHTAGIYGGEPGML
                     VGTRSGHPLSLVQAGTVRARLATATAQGGSETGLLDLFPAGSPLRISHGWTSFPDANA
                     LAAEICNDTQIYKTLMLVGNRSNGGVRRVSVWDRLEVNGPLTAQGVDVSAGVPDHIST
                     DGSFYRWGGQVYLNVDDNLYVRDSQRGIRMHFDTTNGVFKTDVLRLGDKWRMSAIGDA
                     HGNDGWLRLFNVQNTGYFGGLAAGELWSGKALQVSDRRLKDNVREIGDALDKLSRLHG
                     VHFDWREGAPSGVGRPAAGLIAQDVEGVLPEAVALGPDGWKGIDTTAVLALLVQAVKE
                     QQVLIQRLRDELATRA"
     gene            complement(13725..14201)
                     /locus_tag="PKX01_08735"
     CDS             complement(13725..14201)
                     /locus_tag="PKX01_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76250.1"
                     /translation="MSLKDCHGLAGRLHIELRDPRGTLVERRTVNNLITTSGRRLLAS
                     LFAGAAQGPGLQIAVGGSGGEVLPGDTQLGSQVDVAAATIPAIGEDTVDGAPRVVLRV
                     VATLPPTGKPDAQALQEAGILVTVSGAEPVLYNRVTFPLINRAGNLEMTLTWEVIF"
     gene            complement(14227..16080)
                     /locus_tag="PKX01_08740"
     CDS             complement(14227..16080)
                     /locus_tag="PKX01_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76251.1"
                     /translation="MADPRQRRERLLALLPRIYTAQPRDSAVGAVVDALAAALARVDD
                     SLTRVQYDRWVGLASPLREGDDISALEALGRLLQVNRLPPRLAHAGYDPDGPTRLSVR
                     FDAHAPLADALHDLIGDAWRQPDENGRPGDPAALLAEQFPGVRFALDDAARLQADADT
                     PAALARFRALLDPEPGEIFRQRLRVTAQVRTGGLTTPRALLALALADLGAEPCPRLER
                     RQDSTLARGMAPGTRKRCAACAGDGPCPNTPAAVVEAWLTEHPVEARRHTEPAPRLRR
                     VFTLDNPSLLPDRPVWRLTVSDQPAAYPAVRSLESGEITLFAGTLKPGDTLVVQPALS
                     PTERAGFDGYDSPGHHAWSASHPAGRAIVIDADGAERDVSDAVFYLWGTRFDAPEATL
                     DGGMRCGVLDQRVVTPQLRPGSNAWMLLTFAKPDAEFADAGSTVHTSRFAGPQDADGT
                     RFALLDGSLARSDAKFATLLFEAISKTDTASTTEGSTANAARLRLDLEWVARPPATAR
                     LRIPKNEWVAAAAARGVLALVRSDVTGASAAGVRIQVDFPEPVRREYVVSGEGLSLHT
                     GQRWREDAAPSERGPHIGLKLTLRDSAAPQEGHLSTGLVFDTTRLDWSHAG"
     gene            complement(16073..17590)
                     /locus_tag="PKX01_08745"
     CDS             complement(16073..17590)
                     /locus_tag="PKX01_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HMZ76252.1"
                     /translation="MALERVFRKKDFAEVVDAMLGDIASGLGGRVALTDAAEGSVVRT
                     LAEAFARELAVCYEQLDSVYRNAYLDSASGAALDNVVALLGLERQRGGHLEGSVVFGR
                     GQPAPEDIHIPLGTLVAGRGVPLFATTAGSVLDKGERSVLVGVRAVDPGGATVKAGLL
                     THLPRPIAGLESVTNPGDLLLRQREETDEELRARARRLVLSSHTGTVAALEEAVRSLG
                     IREVQVIENPGGRPGEVEVVLGDTDIPADLLEQVRNRLEDVRPAGIVLRCGPATPVFV
                     QVTATLTLNADLPEREQRDIEKRIGDALIAYFGQLKVGEAVRAAKLRALLLGHDAIVG
                     TEPTGQRPLMEPFVWNDGALQSVAFRSLLGNGDVLAGPGERIGLYPEALPLRLSLEPP
                     LLPVQVDADLTLAAGSAVAGLDARVTAVLKDTFERAARRATTGTPADIPYAELRDAVA
                     AALPAAKILRLRVSVIHERDGRVEELDDAAGHATLATREQAMMRRIAVRAEDGHG"
     gene            complement(17580..17993)
                     /locus_tag="PKX01_08750"
     CDS             complement(17580..17993)
                     /locus_tag="PKX01_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76253.1"
                     /translation="MATDPFGTDLRLVFTRGREGADLAAGFSDLESVSGLENLVQALT
                     LRLLVDRGELAGLGHPRYGSRIRDLIGEPLDRPNLELLRRYVRQELLRDPRVAEVVQV
                     AVTPHDERRDGVDVVARVRATDGREARVEVSLDGA"
     gene            complement(18011..18658)
                     /locus_tag="PKX01_08755"
     CDS             complement(18011..18658)
                     /locus_tag="PKX01_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76254.1"
                     /translation="MPAPSPSQLDSPTSGFLKGAGLNGEDVPGLATAIANTAGQALTL
                     FLAQALVMPGIPVAADPISGSGASAGPGRLMPPPAGGPGASQLEGLATAQCQAQGLRG
                     EDADGLAKAIAGTVAQGIALFCAQSMVLPGIAVAGFVSAAPGLLQPVPLASSLEGIAN
                     GLLAQNGLRGEAAPDLAKALAQGVDLALTQFAAQAMVSPGIPCPPGASAGPGRLM"
     gene            complement(18669..19307)
                     /locus_tag="PKX01_08760"
     CDS             complement(18669..19307)
                     /locus_tag="PKX01_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HMZ76255.1"
                     /translation="MSDPIDIIRAVVRDELRSLRLGDLAVVTSIFPHTGDDDGHNHEC
                     SVKLREGGLELRKVPIATPHVGMVGAPRVGDLVLVSYVGGDPNRAVVIGRLYSEKIHP
                     PLHKEDEWHAFAKADGVARYTIDGEESCIVTAGKTIVTVKKDGNVEIQGEADLAIQVK
                     GKVTIQCTDFSLDASGNVDLGQSGAGVITEQSHKCYFTGKALVPSGTVKAKG"
     gene            complement(19348..20400)
                     /locus_tag="PKX01_08765"
     CDS             complement(19348..20400)
                     /locus_tag="PKX01_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HMZ76256.1"
                     /translation="MADTLPAPTSDGRADNPFRAVAILLDAVGRSLEASKRQDQAARW
                     VRIARVREFRDDGIAKGVMKAMDVLTMAVAYLQRFTLDANDLLVQGDAAKALVEVSAE
                     FIKTATSKEFINSLEMAVGQDPSPDSPIPDVANIIDKIVQIADKVPEPDDLKVIGAAL
                     WRLLGIEQASLDEAKLGATTESHIVVERTGKLRLLQFAFDQNFRLQNFGKGAGVSSLD
                     VNRLGGRRLWEAGADKLPSKSVGKYGDAPDIETVWELDFSTDKAGADVQDANSILEAL
                     GYADPAVPDRKVFGAEFAGRLRTFQQINEIPLSGKLDNATLNRLLHLDFDAKNLKRAK
                     RFKADALPKGFDPLKK"
     gene            complement(20406..20951)
                     /locus_tag="PKX01_08770"
     CDS             complement(20406..20951)
                     /locus_tag="PKX01_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76257.1"
                     /translation="MSSTADVVRDLASLVRELGALLETKTAREIPAIARDLGAEAQLG
                     QGVDALASVLGQVRHGVVPLRRMAIGADALVAALGCVPPLVGGLARATDASGEWLATL
                     GLGLGDAAGAAQAVSGPIRQVSDVLDMGEDVAEGAVSLLAAGQWRGLITALDRLDTSL
                     KALKAPPPTPPGAPAGALSGG"
     gene            complement(20948..21829)
                     /locus_tag="PKX01_08775"
     CDS             complement(20948..21829)
                     /locus_tag="PKX01_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HMZ76258.1"
                     /translation="MTVRIGKIELTGVQDLHTEESRTLVEQRVPEQQGSVFQDLGREP
                     LTLVIDGLLLGEDALSALETLRQAHAKAEPQSFAADLVVGTDLTDVIIEDFRVRQVAG
                     YRDRYRFSLRLREHIEPPAAADAAVADVNQGVAADAAGWSDSSLAAAGVLQDPASLSA
                     ALAAQPGLLDTLDMDALGDAVAGNLDSLDAGQLDGMLGALANADPTKAGGLFDALGKA
                     GALGPMLAKYAQEGAAFLKKLDPSKLSSLMRAFSGGLEFLNQLKKVAEAAGKLAGDIK
                     DLRLPPELDRILKGGRP"
     gene            complement(21832..22863)
                     /locus_tag="PKX01_08780"
     CDS             complement(21832..22863)
                     /locus_tag="PKX01_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76259.1"
                     /translation="MNAPARRSAYTVRLGDAVASSTASESRRQLLRLQVVLDMDGPVA
                     ACTVELADAASPPPAIGDAVSVELDGGDGLQTVFTGTVAEAAAHATGQRIVAHGHFAP
                     LGAREVEAAYDSVAADFIVKDLLDQAGATAGTVAAGPDLAAWTLQRSPTALGHLRRLA
                     LLCGADLYADGRGAIHCAVPRSGAADHRFTFGENILRLQLARRVPAFDSIEVWGEGAD
                     QGADKAHWLSTDLAQVSAKASVAADGSVQTGKLGQRPLRLREGALRSGAAAEAVAKAW
                     ATRVAAGRVEGSVEVFAAPKVCPGDLVGIDKLPDDHPAAALLDGPLRVRSVRHELDRQ
                     RGAITRLGF"
     gene            complement(22860..23267)
                     /locus_tag="PKX01_08785"
     CDS             complement(22860..23267)
                     /locus_tag="PKX01_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76260.1"
                     /translation="MPRHDGVATPSPSAAAPSAAAAPPVDEVAPPAPAFLPRHPVLPR
                     IVAALDPVLEQAWRVTDAALPTAAALDAAPPPGTDTAASADPAGAPRVNNHFHVNVAL
                     GSDSAGSGRDPRQLEDALVTLLRDAARRQGLDV"
BASE COUNT         3292 a         7791 c         8742 g         3528 t
ORIGIN      
        1 ccgcttccat gatggcctgg acctgttcga ggttgttgac gttgaacgcg ggcaggccgt
       61 agccgttttc ggcggcgtgg tcgagcagct gacgcatgga aacgagaggc atggtgaaat
      121 ctccggagga ccggcagggg ccggccaggg tgcaagggaa aagcggaatt ctatcgcgcc
      181 ggcatgcctc gtggacgggc cggcgcgggg ccgcgagcgt gatggattac acgggacccg
      241 gtctgggggt gtcgccgacg cgcacgatct tcatggtgtt ggtgccgccg gaggcaccga
      301 tgggttcgcc gatggtgagc acgatgaggt cgccgtaatc gacgacgccc tgctccagca
      361 ggatctgttc ggattcccac aggagcatgt cccggtcggc gtgcatctgg gacatgagca
      421 gcgggtagac ctcccggtag agggtcatct ggttgcgtga ggtgagctcc ggggtgaggg
      481 cgtagatggg cacgccgctg ttgaggcggc tcatccacag ggccgtggag ccggtctggg
      541 tgagggaggc gatggccttg accttgaggt ggtaggcggt ccacatggcg gccatggcga
      601 tggattggtc gatgcgggta aagatccggt cgaggaagtc ccggtccatg gtgacctcgg
      661 cggatttttc ggcttccagg cagatgcgtg ccatggcctc gaccacctcg accgggaact
      721 tgccagaggc cgtctcggcg gagagcatca ccgcgtcggt gccgtcgagc accgcgttgg
      781 ccacgtccga gacctcggcg cgggtcggta ccgggctgtg gatcatggat tccatcatct
      841 gggtggcggt gatggccagc ttgttgcgtt cgcgggcgag ccggatcatg cgcttctgca
      901 gggccggcac ggcggcgtcg cccacttcca cggccaggtc gccgcgggcc accatgatgc
      961 cgtcggaggc gtcgaggatc tcttccaggt tggccacggc ctcggtgcgt tcgatcttgg
     1021 cgatgagcac ggcgctgctg ccggcggcgc gcagcaactg gcgcgccatg tacatgtcgg
     1081 cagcgccctt ggggaaggag atggcgacga agtcgacgcc gatctgggcg gcggtcttga
     1141 tgtcctccat gtccttggcg gtgagggccg gcgcggtgag gccgccgccc tggcggttga
     1201 tgcccttgtt gttggacagc tcgccgccca ccttcacgcg ggtgtggatc tcgtggccga
     1261 ggacgcgctc gacgcccagc ttgaggcggc cgtcgtccag cagcagcacg tcgcccggct
     1321 tcacgtcgcg gggcaggtcc ttgtagtcga ggccgacgcg ttgggcgttg cccagctcgc
     1381 atcttgcgtc gaggatgaag ggggcgtcct tgtcgagggc gatcttgccc tgttcgaact
     1441 tgccgatgcg gatcttgggg ccctgcatgt cggcgaggat gcccaccggg cgaccgacca
     1501 gctgggccgc gtcgcggatc tgggtcgcgc ggcggatgtg gtcttcggcg gtgccgtggg
     1561 agaaattcag gcgcaccacg tccaccccgg tctggacgag gcgttcgagg gtggcgggat
     1621 cggacgaggc gggccccagg gtggcgacga tcttggtgtg acgggacatg gcgtctccag
     1681 gcgatgacag attatggttg tggtcgatgc ggacgtgatt ctaggcgcga atcatgaacc
     1741 ctgctgcggc gcctcgggcg ggacgggctc gccagccagg cggatgccgt tgcgaccgcc
     1801 ggccttggcc tggtacatgg ccgagtcggc ctggcgggtg agggtgtcga tgtcggtgcc
     1861 atccaccgga aagagggcga tgccgatgct gaccgagatc agggcgcggc cttccggcag
     1921 gtcgaagggc tcttcgaggc tctggtggat cttgcgggcg accgcgacgg cctcgtcggc
     1981 gccggccacg tcgtggatca gcgccaggaa ttcgtccccg ccctggcggg ccaggtgatc
     2041 gctggcccgc aggcaggcgc ggatgcggcg ggcggtggcg accagcagct ggtcgccagc
     2101 gtggtggccg aggcggtcat tcacggcctt gaagccgtcc aggtccagga agaagaccgc
     2161 cagacgcgtg ccgcgccggc tcgtcaggcg caggctggcg tccatgagtt ccaggaacat
     2221 cgtgcgattg gccagtccgg tgagcgcgtc atgggtcgcc agatagcgga tgcgctgctc
     2281 cgattccttg cgttcggaga tgtccgtgac ggtgacgatg acgctgagga gcgcaccatt
     2341 gcggccgagc agcggtgcgt cgctgatctg ggtcgtgacg gccgcgccgc gggcgccgat
     2401 cagctcctgc tcgtaggttg cgccttcgcc ccggtgcagg cggcggatga gcgtccggct
     2461 gcggggctcg tcgggcgctg gccacaggct gtcgagacgg cgtccgagca gggcgtcggc
     2521 cgggtgaccg agcagctcgg cgagtcgggg gttcacgaag gtgaagcgca ggccgccgtc
     2581 gacggcggcg atgccgtccc gcgagagatt gaccagggtg ctgtagcggg cttcgctggc
     2641 acgcagggag gcgccgatgc ggtccaggtc cagggcgcgc tggcgagcgc ccgcgaagga
     2701 ggcggtgagc agcacgatga gcgcgccgat caggaccacg gacgtggctt ccaccgtatc
     2761 cacggtcttt cccatgctga aggcgggggt gctctcgaag gcgagggtcc aggtgcgacc
     2821 atccagttcc agcacgtagg tggcctgaaa gcgccccacc tgtgcgagtt ccgggtggct
     2881 gtcgaagagc agggcctccg ccgtccggga ttcgccatcg aagatgcgca gccggatccg
     2941 gtccggcgtg ttgccgaggg tgccggtgat cagatccttc atccggatcg ggctgtacac
     3001 ccagccgacg aaggcctctt cccgctgggc cggggtggcg ggcgaggtgc cgttgcggaa
     3061 gaccggcagg tacaggagca cgccggcctg cgggtcggtc tcggtctcct gcaccagccg
     3121 gaccttgccc gacagggtgg cgagaccgag gctgcgggtc cgtagcatgg cggtgcggcg
     3181 caccggctcg gacagcatgt cgtagccgaa ggcgcggcgg ttgcgccagt cgaagggttc
     3241 gagatagacg atgggcgcgt actccgcgcg ctcacccggc gggctgatgc ggtagtcggc
     3301 aaacccctct tcacggaccg cctgttcgtg ggcgccctga tgtgccgcag cgaccgacag
     3361 ggcgaagccg accccttgaa tgcccggaaa ttccgtatcc agacgcagtt cgcgggcata
     3421 ggccttccat tcgctgcggg acactcgttc agaggccgca aacagcgccc gcgcgccgcg
     3481 tagcacctgg cgataggcct tgaagcgctc gccgatctgg aaatgcatct ccgcagagcg
     3541 gtaggcgaaa tcgagggctt gatcctgctc cttctcctgg gtcaggcggt ggagtccctg
     3601 agacaccccc acaagcgcga gcagcagcgc gatcagcgtc gggacgacga cgctggcctg
     3661 cttccagctg aaggggcggg gggcggaaaa gggcatcctg atcgactcct ggacggtgcg
     3721 cgcacggggc cccggttccg gtgcggcggg cggtgttcct ccggtctacg gccacgatcg
     3781 ggccgacatt gaggagagaa gtgggcgagt ccggaaaagc ccgtcaggca gagcggcggc
     3841 ggtgtcataa tcacgggctg gcgcccgcct ggatggggtg gactgcccgc tttccgttat
     3901 ttctgactcg caaggatcct gcatgattct cgtcaccggc ggcgccggtt tcatcggtgc
     3961 caacttcgtt ctggactggc tcgccacgtg cgacgagccg gtggtcaatc tcgacgccct
     4021 cacctacgcg ggcaaccttg agaacctcgc ctccctgaag ggcgaccccc gtcacgtctt
     4081 cgtgcagggc gacatctgcg accgccccct catcgaccgc ctgctggccg aacaccagcc
     4141 ccgtgcgatc gtccatttcg ccgccgaaag ccacgtggac cgcagcatcc acggccccgg
     4201 cgaattcatg cgcaccaacg tcaccggcac cttcaccctg ctcgaagcgg cccgcgccca
     4261 ctggaacggc ctcgaaggcg ccgccaaggc cgacttccgc ttccaccacg tgagcaccga
     4321 cgaggtctac ggctccctcg gtcccacgga cccggccttc accgagacca agacctacga
     4381 acccaacagc ccctactccg ccagcaaggc cgcctccgac cacctcgtgc gcgcctggca
     4441 tcacacctac ggcctgcccg tcaccaccag caactgctcc aacaactacg ggccctacca
     4501 cttcccggaa aagctcatcc cgctgatgat cgccaacgcc ctggccggca agcccctgcc
     4561 cgtgtacggc gacggcatgc agatccgcga ctggctgtac gtcaccgacc actgctccgc
     4621 gatccgcgcc atcctcgaaa aagggcaacc tggcgaggtg tataacgtgg gcggctggaa
     4681 cgagaagccc aacatcgaga tcgtgcgcac cctgtgcgcc ctcctcgacg aactcaagcc
     4741 cgacccggcc ggttcctacg cacgcctgat cacctacgtc accgaccgtc ccggccacga
     4801 ccgccgctac gccatcgacg cccgcaagat cgagcgcgag ctgggctggc gccccgccga
     4861 gaccttcgat accggcatcc gcaagaccgt cgagtggtat ctggcgcatc aggactgggt
     4921 cgcccacgtg cagagcggcg cctaccggga ctgggtcgag cagaactacg cggcacgctg
     4981 agcgctgggc gagccccggc ccggacgtca gtttttccac cacggatagc ggtgatgccc
     5041 atgaagatcc tgctcctcgg caagaacggc caggtcggct gggagctcca gcgcagtctc
     5101 gcccccctcg gtgacctcgt cgccctggat cgtcacggcg atggcgggtg gatcggcgat
     5161 ctggccgatc cggacggcct cgccgccacc gtgcgggccg tggcgccgga cctcatcgtc
     5221 aatgccgccg cccacacggc ggtggacaag gccgaaagcg agccggatct ggccttcacg
     5281 atcaacgccc tcgcgcccgg cgcgctggca cgggcggcgg ccacctgcgg ggcgcttctc
     5341 gtccactact ccaccgatta cgtcttcgac ggccgcggca ccgcgccgtg ggccgaaggc
     5401 gcccccaccg gccccctctc cgtctacggc cggaccaagc tcgaagggga aaccctcatc
     5461 cgcgacagcg gctgccgcca cctcatcttc cgtaccagct gggtgtatgc cgcccgcggc
     5521 ggcaacttcg ccaggaccat gctgcgtctg gccgtcgagc gggacgccct gagcgtcgtt
     5581 gacgaccagc atggcgcccc caccggcgcc gagctccttg ccgacgtcac cgcccacgcc
     5641 gcgcgcatga ccttcgcccg ccccgaactg gccggcacct atcacctggc ggccgcgggc
     5701 gagaccacct ggcatggcta cgcccgccac gtcgtcgaat tcgcccgcgc ccgcggcgtg
     5761 ccgatccggg ttgcacccga cgcgatccgc ccggtgccca cgagcgcctt cccgaccccc
     5821 gcccagcgcc cggccaactc gcgcctcgac acccgcaagc tgcgcgatgc cttcggcctc
     5881 gtcctgcctg actggcaaca gggcgtcgaa cgcatgctca ccgaaatcct gccgaactcg
     5941 aactgagaaa gaccaccatg caacgcaaag gcatcatcct cgccggcggc tccggcaccc
     6001 ggctgcaccc ggccaccctg gccgtctcca agcagctcat cccggtgtac gacaagccga
     6061 tgatctacta ccccctcagc accctcatgc tggcgggcat ccgcgacatc ctgatcatct
     6121 ccacgcccca ggacactccg cgcttccagc aactgctggg cgacggcagc cagtggggcc
     6181 tcaacctcca gtacgccgtc cagcccagcc cggacggcct cgcccaggcc ttcatcatcg
     6241 gcgcagactt cgtcggcaat gcccccagcg ccctggtgct cggtgacaac ctgttctacg
     6301 gccacgactt cagcagcaat ctgcgcaccg ccagcgcccg gaccgacggc gccaccgtct
     6361 tcgcctatcc ggtgcatgac cccgagcgtt acggcgtcgt cgaatttgac gcccagggcc
     6421 acgccgtcag tctcgaagaa aagcccaggc agcccaagag ccgctacgcc gtcaccggcc
     6481 tctacttcta cgaccagcgc gtcgtggatg tggcccgcgc catccagccc agcccccgcg
     6541 gcgagctgga gatcaccgac gtgaaccgcc aatatctgga atggggggcc ctcgacgtgc
     6601 aggtcatggg ccgcggccac gcctggctcg acaccggcac ccacgagagc ctgctcgaag
     6661 ccagcctgtt catccagacc cttgagaagc gccagggcct caagatcgcc tgccccgaag
     6721 agatcgcctg gcgcgccggc tggatcgacg cggccagact ccagcaactc gccgcgcccc
     6781 tcgccaagaa tggctacggg cagtacctcc tgcgcatcct cgacgaaaaa gtcttctgac
     6841 cggagggtcc gatgaacctc atccaaacgg ccatccccga tctgctgatc ctcgaaccca
     6901 aggtgttcgg cgacgcccgt ggcttcttca tggagagcta caacgcccgg acctttcgcg
     6961 agctcaccgg tctcgacgtg gacttcgtgc aggacaacca ctcccgctcc gccaagggcg
     7021 tgctgcgcgg cctgcactac cagatccagc aggcccaggg caagctggtg cgcgtggtgc
     7081 gcggcagcgt cttcgacgtg gcggtagacc tgcgcaaggc ctcgcccacc ttcgggctgt
     7141 gggcgggggt cgagctgtcg gaagaaaacc agcgccaggt gtggatcccg ccgggttttg
     7201 cccacggttt cctggtcacc agcgacagcg ccgacttcct ctacaagacc accgattact
     7261 acgccccgca gcacgaacgc tgcctgctct ggaacgatcc ggtcgtgggg gtggcgtggc
     7321 ccctcgcgca gatcgacggc gcgccgcagc tctctgccaa ggatgtggcc ggcaagggcc
     7381 tggccgactg cgaggtcttc gcctgaccgg gcggagcggg aacagagtct gacaactatt
     7441 tctttgtgcc ggtcatgatc agcgcgcgat aatgcgcgcc tgtcattagt tcgcccggag
     7501 tgccgcatgt cccagtccct gatctacctc gtcgccggtg cgcgtcccaa cttcatgaag
     7561 atcgcgccca tcgtgcgggc gttgcaggcg cgggccgacc gtttcacctt caagatcatc
     7621 cacaccggcc agcactacga ccgggagatg agcgacgtct tcttcgacga gctgggtatc
     7681 cccaagccgg attaccacct ggaggcgggg ggcggcagcc acgccacgca gaccgccaag
     7741 atcatggtgg ccttcgagga gatctgccag aaggtgcgcc ccgactgcgt gctggtggtg
     7801 ggtgacgtga actccaccct ggcctgcgcc atcgacgcca agaagctgca gatcccggtg
     7861 gcccacgtcg aagccggcct gcgcagcggc gacatgcgca tgccggagga gatcaaccgc
     7921 ctcgtgaccg acagtatctc cgactggttc ttctgcaccg agccggccgg cgtggacaac
     7981 ctgctcaagg aaggcaagca gccggaggcc ctcttccacg tgggccacgt gatggtggac
     8041 aacctgctct accagcgcga ccagctcaat gcatccgaca agtccggcat ggagagtgag
     8101 gccctcaagc gccgttatcc caagtacggc gtggtgaccc tgcaccgtcc ctccaacgtg
     8161 gacgacgaga cccacctgcg cggcatcgcc ggcgcgctca agcagatcgc cgccgacctg
     8221 ccgctgatct ttccggtgca tccgcgcacc cgcggcaacc tggaaaaatt cggactcgat
     8281 ctggggccca acgtggtcct caccaaaccc cagtcctaca tggaattcct caatctgtgg
     8341 aaggacgccg cgctgatcat gaccgacagc ggcggcctgc aggaagaaac caccgcgctg
     8401 ggcattccct gcctcacgct gcgggagaac accgagcgcc cggtcaccat cgacgaaggc
     8461 agcaacgtgc tggtcggccc cgatccggcg cgcattctcg aagaagcggc caaggtgctg
     8521 gccggccagg gcaagcaggg gcgccgcccc gccctgtggg acggcaaggc cgccgagcgc
     8581 atcgtcgccg atctggatcg tctgctgccc tgatgacggc ggtcgccgtc gcggcgatca
     8641 aggccggcgc agggtgaccg tcacatcgcg gatgcgccct tccggcgctg cctcgcccag
     8701 acgcagcgcc agttccacct caccccgcag cacgcagcgg acgcgtcccg atgtccatgg
     8761 gccgtccgcg tcttgttcaa gggccaccac ggacaggcgc ggccgcgtca ggcggacgct
     8821 caggctcggc gccgcgccgg ccagttccac cacattgaag ttttccgccg ccgcgagcag
     8881 tgccgccagg ccggtggcca gcaggctgtc gacggccgga ccgtcgtgtg cccaggcacg
     8941 cagggacagc gccacatggc cgaagcgccg ctcccggtag ccgcggaccg ggaccgtccg
     9001 ggtgctgacc cgcaccggcc caccgggcgc gctgaagaag cgcagggtgc gcccgtcgat
     9061 ctgccaggcg tcacccggcg gcaggatgcg gcccggcggg ctctgcactt ctgcgacctc
     9121 gccgcgggcc gcctcgggca gcggaaaatc cagcggtgag gccggatccg gcgacacctc
     9181 gccctgccag ccggcgcaca ccggatcccg cgtcgggtct tcgccttccg ccccgtccca
     9241 ttccagtttt tccgcccaca gactcacctg ccccgacggt cccgccgggg cgccggccgg
     9301 gccgctccgc aggctggcgt cagggccgag ggcggcacgc agggcaggca ggaacagggc
     9361 ttcaatggcg gcgagcatgc aagttcctca gggcgcggtg tcgagcaggc cgacctccac
     9421 ctgcagccag ggcgcctcgc cgggtccgtc gaggggcaac cagggaccct gaccggtgcg
     9481 ggccagaccg ctctggagcg gtgccgggcg cgggctgtca tccacgtacc acagcagcgc
     9541 cccgctcgtc gcctggcagg tgaggcgcca ggggcccgcc ggggccacgg tttcgggaag
     9601 ccggaagcgc agccagacgg cgtcggctga ctgcgcactg tccggtccga agctcagcgg
     9661 ccactcggcc agcgggggtt cgactgtttg atcgccgcgc aaggcatgca gacgcacgtt
     9721 gcccacgaag gcgtcggcca gagggcgcag gcgcagggcc gtgctcgaca ggctgcggtc
     9781 tgccggcagc ggggcaaacg actgggccgc ctggtgctgc gggtcgatca ggcgagcggc
     9841 ctgcaggggg gcgcgacccg gcggaaacca gggctgttcg ccagggcgtg cctgatgcgg
     9901 gtccactggc ggcgcagcag cgtcgtctgc ttgcgcgggg gcttccaggg tggcggcgaa
     9961 cggtccgacc cgcaccgcct cgtcgccggc ggcccgcagc agcaacggaa tctccgtcgt
    10021 gccgggatga tcgctcaggt agcggttgag gatccgggtg aggccctcca ccggctgggc
    10081 ctgggttggc agcgggccct cgggggcgaa aaaaggcggc aggtcctcca tcgccaggct
    10141 gacatggcag gcctggccgg tgaggatcgc gccgatccgt ttgcccgtca gggcaccggg
    10201 gatggggacg ccgctccggc tgccgcccac atcgccttcg accagcatct cggccatcag
    10261 acgcgaggcg gccagcggcg gaaagcgctg cacggcgccg gtcggcagcg cgtcgagggg
    10321 cgtgaggggc agccagtggc cgcggctgta gagcttcacg cgggcggcgc aggtgcgcgg
    10381 gggcgtggag gcgtcgcggg cgtcgatgat cacgccggtc agggcgcgct cgtggcgcca
    10441 gtcgatggcc gcccagcgcc acgaggcctc ggtctggccg tcggccgcgc cggcatcggt
    10501 ctggaggggc ggatacagca cggcctgttc ggcggcgtcg gccgatgcca gactgaggcg
    10561 ggcctctgca aggcggcggc cggccggcag ggtgacgcgc aggcggatgc cctccgctgc
    10621 ggaaaagctg cgaccggcgg cgggggcgcc gtccggaccg aggagctgga ccgggtccgc
    10681 catgctgggg tcaggcggcg ggtgggggct tggcgggctg ttcggcggac tcgccgagga
    10741 gctcctccag cacctggatg gcgccgctga tgcgcagcag ggtggtgcgc agttcgtcct
    10801 gtttcttctg caggtcggcc atgacgcggg ttccggcgtc gtattcctcg tgcaactggg
    10861 cgaggcgggc ggtgagttcg tttttcatcg gcggggcggt gggtgatggt tcagcggacg
    10921 aagacgaaga cgtcgatggt acccgtgccg ccgtcgaaat cggccagggc cttgccgatc
    10981 agggtgcccg gcggagcgtc tccggcgcgc atggcgtggc cggggcggct ggctgcggtg
    11041 agcaggtcgc cgcgccggat ggggccgttt tcgtcagtga cctggcaggg gacgcggccg
    11101 ctcagggcca cggggacttc gccggcaaag ggcggctggc ggtcggcgtc ggcgcccagg
    11161 aggacgccgg ggcgggtgga caccacgccc agcaccagcg gatcgcgggc ctggcgactg
    11221 cgccggatgc cgtcccggtc cgggtccagg ctgaccacgt cgccgggcgc cagggcctcg
    11281 acggcgtcga agttctcggc cagatcccgg ttgccgctct ggtagccgtt gcgcgaccac
    11341 atggtgcctt cggcctccac gtcccaggta tggatgccgc cgccccagcc gcccgtccgc
    11401 ggactggggg cgaagccatg ggtgcccaga ttgcccgaaa tggtgacgtt gtcgtacatc
    11461 ttgacggcgc gcgacagggg gttctggctg atgctgcggc ccaggatcat cagggcgttg
    11521 taatcgccgc cgtcgttttc gatggcggcg tagccggcgg cgttgccgaa gcccgtgtga
    11581 cggtggcccg tctcggtgaa atacaggtcc gagccgccgg cgtggatcgc gccgcgcaca
    11641 tcgaggcggg cgttgggcgc ggcggtgccg aggcccaggt tggattcgag gatcgcgctg
    11701 accttgctgc cgatcaccgc cacgttgcgc agcgcactgc cgtccgcctc gacgcgcagg
    11761 aagcggaatt cctggcccgc gccgtcaccg ctgaggtgca ggttggcccc tggcttgttc
    11821 gggtcgaggc gcttccaggc gccatcgaaa taggcgttcc agcccaggtt ggtccaggca
    11881 ttgttgcggg cgccgatgtc gagggcgctc agttcgccaa gctgcaggcg cgcgctggca
    11941 agcttctcgc tgccgaccgc caggcgttcg gtgatggcca ggccgccgcg cagcacgtcg
    12001 ccattgcgat tcagcgcgtt tttcaggccg gcgatgaggc cgtcgatctc ggccagcagg
    12061 gcgcgggcat tgaccttgag ctcgccgcgc acgtcgaggg cctgcagtgt gacgctcgca
    12121 gcggggtcga tgcggctgcc gtcgatggcc ttttcggcga tgccttcgcg gggcaccggc
    12181 tgaccctggc cggcacggtg gtcgtgggcg gcgagggcct cggcggcgcg gacctgagcc
    12241 tcgttccagt cgtcggcgcg gatgatgtcg ccggcagcgc gcttgacgta ggggtcggga
    12301 cgggtgggca tggcggcttc cggatcaggc gcgggtcgcg agctcgtcgc gcaggcgctg
    12361 gatgaggacc tgctgttcct tgacggcttg caccagcagg gcgagcacgg cggtggtgtc
    12421 gatgcccttc cagccgtccg gccccagggc gacggcttcg ggcagcacgc cttccacgtc
    12481 ctgggcgatg agcccggccg ccggccgccc gacgccgctg ggggccccct cgcgccagtc
    12541 gaagtgcacg ccgtgcaggc ggctcagctt gtcgagggca tcgccgatct cgcgcacatt
    12601 gtccttgagg cgccggtcgg agacctgcag ggccttgccc gaccacagct cgcccgccgc
    12661 caggccgccg aagtagccgg tgttctgcac gttgaagagg cgcagccagc cgtcgttgcc
    12721 gtgggcgtcg ccgatggccg acatgcgcca cttgtcgccc aggcgcagca cgtcggtctt
    12781 gaacacgccg ttggttgtgt cgaagtgcat gcggatgccg cgctggctgt cgcgcacgta
    12841 caggttgtca tccacgttga gatacacctg cccgccccag cggtagaagc tgccgtcggt
    12901 cgagatgtgg tcgggcacgc cggccgagac atccacgccc tgggcggtga gggggccgtt
    12961 gacctccagc cggtcccaca cggagacgcg gcgtacgccg ccgttgctgc ggttgcccac
    13021 cagcatgagg gtcttgtaga tctgggtgtc gttgcagatc tcggcggcca gggcattggc
    13081 atccgggaag ctggtccagc cgtgggagat gcgcaggggg ctgccggcgg ggaagagatc
    13141 gaggaggccg gtctcgctgc cgccctgggc ggtggcggtg gccaggcggg cgcgcacggt
    13201 gccggcctgc acgaggctca ggggatgacc gctgcgggtg ccgacgagca tgccgggttc
    13261 gcccccgtag atgcccgcgg tgtgggccat cacccgggcg tccacgtcgc cggcgcgcac
    13321 tgccagcttg gcgtgggcgc cgcccaggat atccaccacg cggctccagc cctcggcgtt
    13381 ctccggcgcg tcggtgccca cgcccaggtc gccggccgtg gtgaggcggg cgccggtgaa
    13441 gctggccagc gccgccttga tgtcggcaag ctggctggtg ccggtgatct tgagggggcc
    13501 ggagacggtg aggcccttga gggcgacctt ggcgtccggg tcgatgcggc tgccgtcgat
    13561 ggccttttcg cggatgcctt cccgcgggat ctgctggaag gggtcgccgc tgtggtcgtg
    13621 ggcctggatc tcctggcggg cgcggacctg catctggttc cagtcggcgg cgagcatgat
    13681 gtcgccgggg tccttgcgga cgtagggggt gggggtatcg gccatcagaa gatcacctcc
    13741 caggtgagcg tcatctccag gttgcccgcc cggttgatga gcggaaaggt gacgcggttg
    13801 tagagcaccg gctcggcgcc ggagacggtg accaggatgc cggcttcctg cagcgcctgg
    13861 gcgtcgggct tgccggtcgg cggcagggtg gcgacgaccc gcagcacgac ccgcggcgcg
    13921 ccgtccacgg tgtcttcgcc gatcgcgggg atcgttgccg cggccacgtc gacctggctg
    13981 cccagctgcg tgtcgccggg gagcacctcg ccgccgctgc cgcccaccgc gatctgcagg
    14041 ccagggccct gggctgcgcc ggcaaacagg ctggccagca ggcgccggcc ggacgtggtg
    14101 atgaggttgt tcacggtgcg gcgttcgacc agcgtcccgc gtgggtcgcg cagttcgatg
    14161 tgcaggcgtc cggccaggcc gtggcagtcc ttgagtgaca tggggtgtgc tcctcgggcg
    14221 ggcgggtcag cccgcgtggc tccagtcgag acgggtggtg tcgaacacca ggcccgtcga
    14281 gagatgtcct tcctgcggtg cggcgctgtc ccgcagggtg agtttcagcc cgatatgggg
    14341 gccgcgctcg gatggggcgg cgtcctcgcg ccagcgctgg ccggtgtgca gggacagccc
    14401 ctcgccggag acgacgtact cgcggcgtac cggctcggga aagtccacct ggatgcgaac
    14461 gcccgccgcg ctggcgccgg tgacgtcact gcgcaccagg gccagcacgc cgcgggcggc
    14521 ggcggcggcg acccattcgt tcttggggat gcgcaggcgg gccgtggcgg gcggccgggc
    14581 gacccattcc agatcgagcc tcaggcgcgc ggcattggcg gtgctgcctt cggtggtgga
    14641 ggcggtgtcg gtcttgctga tggcttcgaa gagcagggtg gcgaacttgg catcgctgcg
    14701 cgcaagactg ccgtcgagga gggcgaaacg ggtgccgtcg gcgtcctgcg ggccggcgaa
    14761 acgcgaggtg tgcacggtgc tgccggcatc ggcaaattcc gcgtcgggct tggcaaaggt
    14821 gaggagcatc caggcgttgc tgcctggccg cagctggggc gtgacgacgc gctggtcgag
    14881 cacgccgcag cgcatcccgc cgtcgagggt cgcctcgggg gcgtcgaagc gggtgcccca
    14941 caggtagaag acggcgtcgc tgacgtcccg ctcggcgccg tcggcgtcga tgacgatcgc
    15001 ccggcccgca gggtgggatg cgctccaggc gtggtgcccc ggtgagtcat agccgtcgaa
    15061 gccggcccgt tcggtggggc tcagggcggg ctggacgacg agggtgtcgc cgggtttgag
    15121 ggtgccggcg aagagggtga tctcgccgga ttccaggctg cgcacggcgg ggtaggccgc
    15181 aggctggtcg ctgacggtca ggcgccagac cggccggtcg ggcagcaggc tggggttgtc
    15241 gagggtgaat acgcggcgca ggcgcggcgc cggttcggtg tggcggcggg cttcgacggg
    15301 atgttcggtg agccaggcct cgaccacggc ggccggggtg ttggggcacg ggccgtcgcc
    15361 ggcgcaggcg gcgcagcgtt tgcgggtgcc cggcgccatg ccgcgggcga gggtactgtc
    15421 ctggcgtcgt tcgaggcggg ggcagggctc cgcgcccagg tcggcgaggg ccagtgcgag
    15481 cagggcccgc ggggtcgtga ggccgccggt gcgcacctgg gcggtgacgc gcaggcgctg
    15541 gcggaagatc tcgccgggct ccgggtcgag cagggcgcgg aagcgggcga gggcggcggg
    15601 cgtgtcggcg tcggcctgga ggcgggcggc gtcgtccagg gcgaagcgca cgcccgggaa
    15661 ctgctcggcg agcagcgccg cggggtcgcc ggggcggccg ttctcgtcgg gctggcgcca
    15721 ggcgtcgccg atcaggtcgt ggagggcgtc ggcgaggggg gcgtgggcgt cgaagcgcac
    15781 gctcaggcgg gtcgggccat ccgggtcgta gccggcgtgg gcgaggcgag gcggcaggcg
    15841 attgacctgt agcagacggc cgagggcttc cagggcgctg atgtcgtcgc cctcgcgcag
    15901 ggggctggcg aggccgaccc agcggtcgta ctggacgcgg gtgaggctgt cgtccacccg
    15961 cgccagggcg gcggcgaggg cgtccaccac ggcgcccacg gcgctgtccc gcggctgggc
    16021 ggtgtagatg cgtggcagaa gggcgaggag gcgctcccgg cgctggcgcg gatcagccat
    16081 ggccgtcctc ggcacgcacg gcgatgcgcc gcatcatggc ctgctcgcgg gtggcgaggg
    16141 tggcgtggcc ggccgcgtcg tccagttcct cgacgcgtcc gtcgcgctcg tggatgacgc
    16201 tgacccgcag gcgcaggatc ttggccgccg gaagggcggc ggccacggcg tcgcgcagct
    16261 cggcgtaggg aatgtcggcc ggtgtcccgg tggtggcccg gcgcgcggcc cgctcgaagg
    16321 tgtccttgag cacggccgtg acgcgggcat cgaggccggc cacggcgctg ccggcagcca
    16381 gcgtgagatc cgcgtccacc tgcaccggca gcaggggcgg ttccaggctg aggcgcaggg
    16441 gcagggcttc cggatacagc ccgatgcgct cgcccggccc ggccagcacg tcgccgttgc
    16501 cgagcaggct gcggaaggcc acggactgga gcgcgccgtc gttccacacg aaaggctcca
    16561 tcagtgggcg ctggccggtg ggctcggtcc cgacgatggc gtcgtggccg aggagcaggg
    16621 cgcgcagctt ggctgcgcgc accgcctcgc ccaccttgag ctggccgaaa taggcgatca
    16681 gggcgtcgcc gatgcgcttt tcgatgtcgc gctgttcccg ctcgggcagg tcggcgttga
    16741 gggtgagggt cgcggtgacc tgcacgaaga ccggcgtggc ggggccgcag cgcagcacga
    16801 tgccggccgg gcggacgtcc tccaggcgat tgcgcacctg ctccagcagg tcggcgggga
    16861 tgtcggtgtc gcccagcacc acctccacct cgccgggacg gccgccgggg ttttcgatca
    16921 cctgcacttc gcggataccc aggctgcgga cggcttcctc cagcgcggcg acggtgcccg
    16981 tatgggagga caggacgaga cggcgggcgc gggcgcgcag ctcctcgtcg gtctcctcgc
    17041 gctggcgcag gagcaggtcg ccggggttgg tgacgctctc cagaccggcg atggggcgcg
    17101 ggagatgggt gagcaggccg gccttgacgg tggcgccgcc cggatcgacg gcacgtacgc
    17161 ccaccagcac gctgcgctcg cccttgtcga gcacgctgcc cgccgtggtg gcgaagaggg
    17221 gcacgccgcg gccggcgacc agggtgccga ggggaatgtg gatgtcctcc ggcgcaggtt
    17281 ggccgcggcc gaacacgaca ctgccttcca ggtgtccgcc gcgctggcgt tccaggccga
    17341 gcagggccac cacgttgtcg agggcggcgc cgctggcgct gtccaggtag gcattgcgat
    17401 agacgctgtc gagctgctcg tagcacaccg ccagctcccg cgcgaaggct tcggcgaggg
    17461 tgcgcaccac gctgccctcg gccgcgtcgg tgagcgccac acggccaccc aggccggaag
    17521 cgatgtcgcc cagcatggcg tccaccacct cggcgaaatc cttcttgcgg aagacccgtt
    17581 caagcgccat cgaggctcac ctccacgcgg gcttcgcggc cgtcggtggc gcgcacgcgg
    17641 gccaccacgt ccacgccgtc gcggcgttcg tcgtggggag tgaccgccac ctgcacgacc
    17701 tcggcgacgc gcgggtcgcg caacagctcc tggcgcacgt agcggcgcag gagttccagg
    17761 ttggggcggt cgaggggctc gccgatcagg tcgcggatgc ggctgccata gcgggggtgg
    17821 cccaggccgg cgagttcgcc ccgatccacc agcaggcgca gggtgagagc ctggacgaga
    17881 ttctccaggc cgctgacgct ctccagatcg ctgaagccgg ccgccaggtc ggcgccttcc
    17941 cggccgcggg tgaagaccag gcgcagatcg gtgccgaagg ggtcggtggc catgggcgcg
    18001 gtccgtgggc ttacatgagc cggcccgggc cggcgctggc gccgggcggg cagggaatgc
    18061 cgggcgacac catcgcctgg gcggcaaact gggtgagggc cagatccacg ccctgggcga
    18121 gagccttggc gagatccggc gccgcctcgc cacgcaggcc gttctgggcg aggaggccgt
    18181 tggcgatgcc ttccagcgac gacgccaggg gcaccggctg gagcaggccc ggcgcggcgc
    18241 tgacgaagcc ggccacggcg atgcccggca gcaccatgga ctgggcgcag aacagcgcga
    18301 tgccctgggc gacggtgccg gcgatggcct tggcgaggcc gtcggcgtcc tcgccccgca
    18361 ggccctgggc ctggcactgg gcggtggcga ggccttcgag ttgggacgcg ccggggccac
    18421 ctgccggcgg cggcatcaga cggccgggac cggcgctggc gccgctgccg ctgatcgggt
    18481 cggccgccac ggggatgccg ggcatcacca gggcctgggc gaggaagagg gtcagggcct
    18541 ggccggcggt gttggcgatg gccgtggcga ggcccgggac atcctcgccg ttgaggccgg
    18601 cgcccttgag gaagccgctg gtcggcgaat cgagctggga ggggctgggg gcgggcatgg
    18661 gcgggatcct agcctttggc cttgacggtg ccggagggca ccagggcctt gccggtaaag
    18721 taacacttgt gggactgctc ggtgatcacg ccggcgccgc tctggcccag atccacgttg
    18781 ccggaggcgt cgaggctgaa gtcggtgcat tggatcgtga ccttgccctt gacctggatg
    18841 gccaggtcgg cttcgccctg gatctccacg ttgccgtcct tcttcaccgt gacgatggtc
    18901 ttgccggccg tgacgatgca ggattcctcg ccgtcaatgg tgtagcgggc gaccccgtcg
    18961 gccttggcga aggcgtgcca ctcgtcctcc ttgtggaggg gcgggtggat cttttcggaa
    19021 tacagccggc cgatcaccac cgcccggttg gggtcgccgc ccacgtagct gacgaggacc
    19081 agatcgccca cccgcggcgc gccgaccatg cccacgtggg gcgtggcgat gggcaccttg
    19141 cgcagttcga gcccgccttc gcgcagcttg accgagcatt cgtggttgtg gccgtcatcg
    19201 tcgcccgtgt gggggaagat gctggtgacc accgccaggt cgcccaggcg caggctgcgc
    19261 agctcgtcgc ggacgacggc gcggatgatg tcgatcgggt cgctcatggc ggcaggcgaa
    19321 aggacgggcg atgaggtcgg cggtcgctca cttcttcagc ggatcgaagc ccttgggcag
    19381 ggcgtcggcc ttgaagcgct tggcgcgctt caggttcttg gcgtcgaagt ccaggtgcag
    19441 cagacggttg agggtggcgt tgtcgagctt gccgctcagg ggaatctcgt tgatctgctg
    19501 gaaggtgcgc aggcggccgg cgaattcggc gccgaagacc ttgcggtccg gtaccgccgg
    19561 gtcggcgtag ccgagggctt cgaggatgga gttggcgtcc tgcacgtcgg cgcccgcctt
    19621 gtcggtggag aaatccagct cccacacggt ctcgatgtcc ggcgcgtcac cgtacttgcc
    19681 cacggacttg gacggcagct tgtcggcgcc ggcttcccac aggcggcggc cgcccaggcg
    19741 gttcacgtcg agggaggaca cgccggcgcc cttgccgaag ttctgcaggc ggaagttctg
    19801 gtcgaaggcg aactggagca ggcgcagctt gccggtgcgc tccacgacga tgtggctctc
    19861 ggtggtggcg cccagcttgg cctcgtcgag gctggcctgt tcgatgccca gcaggcgcca
    19921 cagggccgcg ccgatcacct tgaggtcgtc cggctcgggc accttgtcgg cgatctgcac
    19981 gattttgtcg atgatgttgg ccacgtccgg gatgggcgaa tcggggctcg ggtcctgacc
    20041 gacggccatt tcgagggagt tgatgaactc cttgctggtg gcggtcttga tgaattcggc
    20101 cgagacctcc accagggcct tggcggcgtc gccctggacg agcaggtcgt tggcgtcgag
    20161 ggtgaaacgc tgcaggtagg cgacggccat ggtcagcacg tccatcgcct tcatgacgcc
    20221 cttggcgatg ccgtcgtcgc ggaattcgcg cacgcgggcg atgcgcaccc agcgggcggc
    20281 ctggtcctgg cgcttgctgg cctccaggga gcggcccacg gcgtcgagca ggatggcgac
    20341 ggcgcggaag gggttgtcgg cgcggccgtc ggaagtgggg gcgggtaagg tatcggccat
    20401 gggggtcatc ctccggaaag ggcgccggcg ggggcgccgg gcggggtggg gggcggcgcc
    20461 ttgagcgcct tgagggatgt atcgaggcgg tcgagggcgg tgatgagacc gcgccactgt
    20521 cccgcggcga gcaggctgac agcaccttcg gccacgtctt cgcccatgtc gaggacatcc
    20581 gacacctggc ggatcgggcc ggagacggcc tgtgcggcgc cggccgcgtc cccgaggccg
    20641 aggcccaggg tcgccagcca ttcgccggag gcgtcggtgg cgcgggcgag gccgcccacc
    20701 aggggcggga cgcagcccag ggcggcgacc agggcgtcgg cgccgatggc catcctgcgc
    20761 aggggcacga cgccgtgacg gacctgaccg agcacggagg ccagcgcatc cacgccctgg
    20821 ccgagctggg cctcggcgcc caggtcacgg gcgatggcgg gaatctcgcg ggcggtcttt
    20881 gtctccagca gggcaccgag ctcccgcacc agggacgcca ggtcgcggac cacgtcggcg
    20941 gtgctgctca tggccggccg cccttgagga tgcggtccag ttcgggcggc aggcgcaggt
    21001 ctttgatgtc gcctgcgagc ttgccggcgg cctcggccac cttcttgagc tggttgagga
    21061 actccagccc gccagagaag gcgcgcatca gcgaactgag cttggatggg tcgagcttct
    21121 tgaggaaggc cgcgccttcc tgggcgtatt tggcgagcat ggggccgagg gcaccagcct
    21181 tgccgagggc gtcgaagagg ccgccagcct tggtcggatc cgcgttggcg agggcgccca
    21241 gcatgccgtc gagctggccg gcgtcgaggc tgtccagatt gccggccacg gcgtcgccga
    21301 gggcgtccat gtccagggtg tcgagcaggc cgggctgggc ggcgagggcc gcggacaggg
    21361 aggccgggtc ctgcagcacg ccggccgcag cgaggctgct gtcgctccag ccggcggcgt
    21421 cggcggccac gccctggttc acgtcggcga cggccgcgtc ggcggcggcc ggcggttcga
    21481 tgtgttcgcg caggcgcagg ctgaagcggt agcggtcccg gtagccggcc acctggcgga
    21541 cgcgaaagtc ctcgatgatg acgtcggtga ggtcggtgcc caccaccaga tcggcggcga
    21601 aggactgggg ttcggccttg gcgtgggcct ggcgcagggt ctcgagtgcc gacagggcgt
    21661 cctcgcccag cagcagaccg tcgatgacca gggtgagggg ctcgcggcca aggtcctgga
    21721 aaacgctgcc ctgctgctcg ggcacgcgct gttcgacgag ggtgcgcgat tcctcggtgt
    21781 gcaggtcctg cacgcccgtg agttcgatct tgccgatgcg cacggtcatg gtcagaaccc
    21841 cagccgggtg atggcgccgc gctggcggtc gagctcgtga cgcacgctgc gcacccgcag
    21901 gggaccgtcg aggagggcgg cggcggggtg gtcgtccggc agtttgtcga tgccgacgag
    21961 gtcgccaggg cacaccttgg gcgcggcgaa gacttcgacg ctgccttcca cccggcccgc
    22021 ggcgacccgc gtggcccagg ccttggcgac ggcttcggcg gcagcgccgc tgcgcagggc
    22081 gccttcccgc aggcgcaggg ggcgttgccc gagctttccg gtctgcacgc tgccgtcggc
    22141 ggcaacggac gccttggcag acacctgcgc caggtcggtg ctcagccagt gggccttgtc
    22201 cgcgccctgg tcggcgcctt ccccccacac ctcgatgctg tcgaaggccg gcacgcggcg
    22261 cgccagctgc aggcgcagga tgttctcgcc gaacgtgaag cggtgatccg ccgcgccgct
    22321 acgggggacg gcgcagtgga tggcgccgcg gccgtcggcg tagaggtcgg cgccgcagag
    22381 cagggccagg cggcgcagat ggccaagcgc ggtggggctg cgctgaagcg tccacgccgc
    22441 cagatccggc ccggcagcga ccgtgccggc cgtggcgcca gcctggtcca gcaggtcctt
    22501 gacgatgaaa tctgcggcga cgctgtcgta ggcggcttcc acctcgcggg caccgagggg
    22561 cgcgaagtgg ccgtgggcga cgatgcgctg gccggtggcg tgggcagcgg cttcggcgac
    22621 ggtgcctgtg aagacggtct gcaggccgtc gccgccgtcg agctcgacgc ttaccgcgtc
    22681 gccgatggcg gggggcgggc tggcggcgtc ggccagttcg acagtgcagg cggcgaccgg
    22741 gccgtccatg tcgagcacca cctgcagacg caggagctgg cggcgggact cgctggccgt
    22801 gctgctggcc accgcgtcgc caaggcggac cgtgtaggcg ctgcgccggg ccggggcgtt
    22861 catacgtcca gtccctgacg gcgggccgcg tcgcgcagca gggtgacgag ggcgtcttcg
    22921 agctgccggg gatcgcggcc gctgcctgcg ctgtcgctcc cgagggcgac gttgacgtgg
    22981 aaatggttgt tgacccgcgg ggcgccggct gggtctgcag acgctgcggt gtccgtgccg
    23041 gggggcgggg cggcgtcgag cgcggcggcc gtgggcaggg ctgcgtcggt gacgcgccag
    23101 gcctgctcca gaacgggatc gagggcggcc acgatgcggg gcaggacggg atggcggggc
    23161 aggaaggccg gtgccggggg cgcgacctcg tcgaccgggg gtgccgccgc ggcggacggc
    23221 gcggcggcag agggcgaagg tgtggcgacc ccatcgtggc ggggcacggc ggggcgccgg
    23281 ctgggtctgc agacgctgcg gtgtccgtgc cgggggcgcg acctcgtcga ccgggggtgc
    23341 cgccgcggcg gac
//
LOCUS       DAOQPA010000050        23176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1051_length_23176_cov_19.332555, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000050 DAOQPA010000000
VERSION     DAOQPA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1051_length_23176_cov_19.332555"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..1454)
                     /locus_tag="PKX01_08790"
     CDS             complement(453..1454)
                     /locus_tag="PKX01_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HMZ76261.1"
                     /translation="MNSLLDPAILFFLFGVAAGLLKSNLEIPPAISRFLSLYLLMALG
                     LKGGFALAHSGLTAQVASSLLVAVSLAVIVPLVGFTVLRRKVGGFDAAAIAATYGSVS
                     AVTFITTTQYLESHGYPFGGHMAAALALMESPAIVLAVLLANHLRQSATAGVQVHADG
                     TATLGPAPGGVSFGKVLHESLTDGAQLLLLGAMVVGIVTGDAGKAAMQPFSGDLFKGM
                     LAFFLLDMGLLAARQLPQLRGASPWLPAYALLAPLVHASLALGLCLLAGIEAGNAALL
                     MVLAASASYIAVPAALRFAIPEARPSLYVGMSLGLTFPFNILLGIPLYVSIAHGVLG"
     gene            1547..2458
                     /locus_tag="PKX01_08795"
     CDS             1547..2458
                     /locus_tag="PKX01_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76262.1"
                     /translation="MYATFRQLQLFLAVAEHGSISAAAKACHVTQPTVSMQLRELADS
                     VGLPLYEVIGKRVFLTEAGQLVRQSALAIRDEWAALGTRVDALRGLQRGCLRVALVST
                     AKYFVPTLLGAFCRQYPDVEVSLSILNRDGVLERLQANADDLCIMSLPPDGDEVSREA
                     FLPNRLVVVAPAGHPLAAEQAIAPAALAGERFILREPGSGTRRACERHFATLGWQPTV
                     RMALGSNEAIKHSVAAGLGLAVLSRHTLHADPAVDGLAILDVAGFPIHANWYIVQPRG
                     KRLSPIAAEFLQHLREYVDSLPATTRA"
     gene            2513..2851
                     /locus_tag="PKX01_08800"
     CDS             2513..2851
                     /locus_tag="PKX01_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HMZ76263.1"
                     /translation="MLELARIFGLFVLTALAEITGCYLPWLVWTRGHTPWLLLPAALS
                     LGLFAWLLTLHPGAAGRIYAAYGGVYVAVALLWLWQVDGVRPSRWDWIGSAVCLAGMA
                     IIALQPGREG"
     gene            complement(2877..4052)
                     /locus_tag="PKX01_08805"
     CDS             complement(2877..4052)
                     /locus_tag="PKX01_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ76264.1"
                     /translation="MSSTRTEAPPAVEPAGLPRRLRGALLRILPLLGGLHAYLGWRLL
                     PALDLGALGLSLAIAGLVLSAGLIPLGLLGRFLIPHPGWADRLSGLGGLLMGAFSTLL
                     VLTVLRDGLLWIANDPALAAPTALGVLGLAAFVTAVGYVNARRVARVRRVDLPLADLP
                     DALAGFTLVQLSDLHVGPTIKGDYVQAIVDRVNGLDADLVVLTGDLVDGRVDHLRAHT
                     APLGRLRSRHGPYMVTGNHEYYSGAAAWMREFERIGLRGLHNRHVLIPHRGARFVLAG
                     VTDYSAGVFDPAQASDPVAALAGSPPGLLRILLAHQPRSAAAAEAAGFHLQLSGHTHG
                     GQFWPWNHFVPLQQPFTAGLHRFGRMLVYTSRGTGYWGPPKRFGAPSEITLLRLVKA"
     gene            complement(4132..4527)
                     /locus_tag="PKX01_08810"
     CDS             complement(4132..4527)
                     /locus_tag="PKX01_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76265.1"
                     /translation="MMNCSTSRHPRSAARCIKIGLLVVAGVAAVTGVVMLLWNALLPQ
                     LFAGVSPIGYWQALGVLVLSRLLFGGLRGGGHGRWHAHRAQWEHLTPEEREQFKGRFH
                     RRWGCCGSADAADASAARAEPGDKSADPA"
     gene            complement(4540..5088)
                     /locus_tag="PKX01_08815"
     CDS             complement(4540..5088)
                     /locus_tag="PKX01_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ76266.1"
                     /translation="MAERNRHLTETISRESGRLGRFIRQRVPDPGDAEDILQDVFFEL
                     VEAWRLPEPIEQVGAWLFRVARHRIVDRFRKRREEPLPLAPEGDDDDTEHWLAQALPT
                     TDGGPEAAHLRRQWLDAIAVALDELPPGPRDVFVAHEIDGRSFKDMAAESGVPLNTLL
                     GWKRQAVLHLRARLAPLYLDHS"
     gene            complement(5375..7213)
                     /gene="glmS"
                     /locus_tag="PKX01_08820"
     CDS             complement(5375..7213)
                     /gene="glmS"
                     /locus_tag="PKX01_08820"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMZ76267.1"
                     /translation="MCGIVTAIARRNIVPVLLEGLRKLEYRGYDSAGLAVLKTDGAPA
                     LQRLRAVGRVAELSAQADATALSASIGIAHTRWATHGVPSERNAHPHISGGLAVVHNG
                     IIENFEELRAALQAKGYVFTSETDTEAIAHLLHDTLKTVSDLYEAVRVTIAQLVGAYA
                     IGIVSEAYPDRVIVARKGSPLLLGIGEDGHYAASDTAALLQVTRKVVYLEDGDVAELT
                     LDGYRVTLADGTPVERPVNISSLSADAVELGKYRHYMQKEIFEQPQALANTLEPIAGA
                     GAISPQIFGAEAVDVLKATRRVLILACGTSYHSGFTARYWLESVARIPCTVEIASEYR
                     YRESVPDPDTLVVVISQSGETADTLAALKHAKGLGMTKTLAICNVPESAIVRESALRL
                     ITRAGPEIGVASTKAFTTQLAALALLALTLAKINGRLGAEQESGYLTQLRHLPVAVAK
                     VLELEPEIEKWAQRFAGKHHALFLGRGSHWPIAMEGALKLKEISYIHAESYAAGELKH
                     GPLALVDKEMPVIAVAPADELLEKLKSNLQEVRARGGELYVFADAGSEIPESEGVHIL
                     HMPEHYGMLSPILHVIPLQLLSYHAALVKGTDVDKPRNLAKSVTVE"
     gene            complement(7282..8649)
                     /gene="glmU"
                     /locus_tag="PKX01_08825"
     CDS             complement(7282..8649)
                     /gene="glmU"
                     /locus_tag="PKX01_08825"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HMZ76268.1"
                     /translation="MKVIVLAAGQGKRMRSILPKVLQPLAAKPLLAHVLDTARALQAE
                     RIVVVYGHGGEVVREALDAPDLAWARQDPPQGTGHAVQQAMPHVDDGDTALILYGDVP
                     LIGEATLRRLVGATGPEKLALLTVGMDDPTGYGRILRDAAGKVTSIVEEKDATPEQRQ
                     VREVNTGILAAPVTQLRAWLARLKNDNAQGEYYLTDIIAMAVADGFEVVTTQPDAFEE
                     TLGVNNKTQLAELERIHQRAIARRLTDAGVTVIDPARIDVRGELICGRDVEIDVNCVF
                     EGRVELADGVRIGANCVLRNATLGAGTRVAPYSHIEDTTMGPACIIGPYARTRPGTVL
                     GEDVHVGNFVEIKNSVIADHSKANHLAYVGDADIGQRVNVGAGTITCNYDGANKFRTV
                     IEDDVFIGSDTQLVAPVRVARGATLGAGTTLTKDAPAEQLTVSRARQVTVPGWTRPVK
                     AAKKS"
     gene            8857..10269
                     /locus_tag="PKX01_08830"
     CDS             8857..10269
                     /locus_tag="PKX01_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HMZ76269.1"
                     /translation="MNTPNHDFIENRTFDEIAVGDSAHLVRTLRPEDIHLFAVMSGDV
                     NPTHVDPEFARSSQFREVVGHSMWGSVIISSILGTEFPGPGTVYVGQTLKFWRPITIG
                     DTLTVTITCTHRFEHNHHLLLDCQCVNQDGLKVIDGTAEVMAPTEKIKRPRVALPEIT
                     ISDRQQRYLHALELTKGLAPIPIAVAHPCDTESLRGPLQARDAGLVIPVLVGPEAKIR
                     EVAEREHLDLNGVRIVNVPHSHASAETAVRLARTGEVEALMKGSLHTDELMSEVVAKE
                     TGLRTSRRISHVFMADVPTYPHPLLITDAAINIEPSLEDKVHIIQNAIELAIMMGVPE
                     PKVAILSAVETVNVKIRSTLDAAALCKMADRGQIKGGLLDGPLAFDNAVSLIAAKTKG
                     IRSAVAGQADILVVPDLESGNMVAKQLEYLADALMAGVVLGARVPIVLTSRADTAETR
                     TASCAIAQLMAHKRREGMRK"
     gene            10266..11465
                     /locus_tag="PKX01_08835"
     CDS             10266..11465
                     /locus_tag="PKX01_08835"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HMZ76270.1"
                     /translation="MKAILILNAGSSSLKFALFPLAPRLADAPRLSGQVEGIGAEPLM
                     HAKDTHSGERFTEALPIAPGTDQNTQHRNALEFVFDWIRRHSPGVDIVAAGHRIVHGG
                     DQYGAPVALTPDVVGALETLIPLAPLHQPHNLRAIKSLFTLMPDVRQVGCFDTAFHRT
                     RLPVAERFPLPRALFDEGVKRYGFHGLSYEFVSRQLPDLLGEEKAHGPIVVAHLGNGA
                     SMCALRDGLSRDTSMGFTAVDGLMMGTRTGSLDPGVILHLIEQKGMDAKALSNLVYKQ
                     SGLLGVSGISQDMRALLESDEIAAQEAIELFCYRAACMIGQLTMAAGGLDALVFTGGI
                     GEHAAPIRARIAEWLAWTGLHLYPAANQRHATRIHAKDSSVEVLVVPTNEEWMIAHHT
                     VELLGLA"
     gene            complement(11587..13050)
                     /locus_tag="PKX01_08840"
     CDS             complement(11587..13050)
                     /locus_tag="PKX01_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HMZ76271.1"
                     /translation="MGKPTGFLEFQRLSEAYAPVEERLKHYKEFVCTLKDDEAARQGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVFRGQWEQAIEVLHSTNNFPDFTGRICPAPCE
                     AACTLNINTDAVGIKSIEHAIIDKAWESGWIKPQPAAVKTGKKVAIVGSGPAGLAAAQ
                     QLARVGHDVTVFEKSSRIGGLLRYGIPDFKMEKSHIDRRVAQMEAEGVTFRTQVCVGA
                     KPGLTGINDDSVEVVSADQLTREFDAVILAAGSEVPRDLPVPGRELKGVHFALELLIP
                     QNKEVAGEDKNPISAAGKHVIVIGGGDTGSDCVGTSNRHGALSVTQFEVMPQPPAEEN
                     RPLTWPYWPIKLRTSSSHDEGCNREFAVSTKEFIGENGQVKALRTVQLEWKDGKPSEV
                     PGSVRELPADLVLFAMGFTNPVASLLEAFGVDKDGRGNAKASTDGVGCYATNVPKVFA
                     AGDVRRGQSLVVWAIREGRQAAREVDTFLMGSSVLPR"
     gene            complement(13052..17725)
                     /locus_tag="PKX01_08845"
     CDS             complement(13052..17725)
                     /locus_tag="PKX01_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HMZ76272.1"
                     /translation="MDLPKQQGLYDPANEHDACGVGFIAHIKGKKSHDVVTQGLEILK
                     NLDHRGAVGADPLQGDGAGILIQIPDLLFREEMAKQGVTLPPPGDYGVGMVFLPKEHA
                     SRLACEEEIERAARAEGQVVLGWRDVPIDPAMPMSPTVKAKEPVIRQVFIGRGPDVMV
                     TEALERKLYVIRRRAANAIGSLRLKHSKEFYTPSMSARTINYKGLLLADQVGQYYLDL
                     QDARCDSALALVHQRFSTNTFPTWHLAHPFRYIAHNGEINTVRGNYNWMRAREKGTHS
                     PLLGDDLYKLWPLIYPGQSDSASFDNALELLVMSGYSLAHAMMMMIPEAWESHTLMDA
                     KRRAFYEYHAAMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLVTDDDYVVMASES
                     GVLPIPDEKIVKKWRLQPGKMFLIDMEQGRIIDDKELKDTLAAAKPYQEWIRRINIRL
                     DACPDPAGSAAPEYAASLLDRQQAFGYSQEDIKFILEPMAKSGEEGTGSMGNDSPLAV
                     LSARPKVLYMYFRQLFAQVTNPPIDPIREQLVMSLVTFVGPRPNLLGINEINPPYRLE
                     VPQPVLTVEDMAKLREIARYTEDKFRSAELDICYPIAWGKEGVEARLASLCAEAEDAV
                     LNGSNILIVSDRKVDALNVAIPALLATSAVHQHLVAKGLRTRTGLVVETGSARETHHF
                     AVLAGYGAEAVHPYLALETLSQMAGDAETAEKYVKHFIKGVGKGLMKVMSKMGISTFM
                     SYTGAQIFEAVGLQKSLVEKYFTGTSSQVEGIGVFEVMDEAIRLHQQAFSADPVLAGM
                     LDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSGKYDTYKEYARIINDQSKRHMTLRGL
                     FEFKPAGAPVPLEEVESAKDIVKRFATGAMSLGSISTEAHTTLAIAMNRIGGRSNTGE
                     GGEDPKRFIPIAEPTKLSEVIGASRIERDIELKAGDSLRSSIKQVASGRFGVTTEYLV
                     NADQIQIKMAQGAKPGEGGQLPGHKVSEYIGFLRHSVPGVGLISPPPHHDIYSIEDLA
                     QLIHDLKNANPAAGISVKLVSEVGVGTVAAGVAKAKADHVVIAGHDGGTGASPWSSIK
                     YAGSPWELGLAETQQTLVLNRLRSRIRVQVDGQIKTGRDVVVGALLGADEFGFATAPL
                     VVEGCIMMRKCHLNTCPVGVATQDPTLRKRFSGQPEYVVNYFFFIAEEVREIMAQLGI
                     RKFDELIGRADLLDMRAGIDHWKARGLDYSRIFHLPNVPAAVPRLHTETQDHNLAKAL
                     DNQLIELAKPALEKAEAVNIELPVRNINRTVGAMLSGRVAAKYGHAGLPDDTIHIRLN
                     GTAGQSFGAFLARGVTLDLVGEGNDYVGKGLSGGRIVVRPAAGFRGETPSNIIIGNTV
                     MYGATEGEAYFAGVGGERFAVRNSGATAVVEGVGDHGCEYMTGGTVVVLGMTGRNFAA
                     GMSGGVAYVLDTDGTFESRCNMAQVALEPVEEELAARQGSESGDELEAHGRVDVRHLG
                     MADEALLKQLVENHARYTGSERAKAILADWSEYRAKFVKVMPHEYRRALAEMAAQKQQ
                     ELEAA"
     gene            complement(17960..19084)
                     /locus_tag="PKX01_08850"
     CDS             complement(17960..19084)
                     /locus_tag="PKX01_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HMZ76273.1"
                     /translation="MSVLAPYAVTEAVSRGRRIPEAPPAARGEFQRDRDRIVHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSIEVAQITRTIARALHLNEDLAEAVALAHDLGHTP
                     FGHAGQEALNACMKDYGGFEHNLQSLRTVDVLEESYAAFDGLNLMFETREGILKHCSP
                     KNAELLGDVGRRFLEGRQPSLEAQLANLADEIAYNNHDVDDGLRSGLITLEQLDDVPV
                     FARNRRSVEQEYPGLSGRRLVHETIRRMINLMAVDLIRQSTTNIAASGVASTGDVASA
                     PRLLAFSADLLPDLRVLKTFLRENLYWHYQVLRMTDKARRIIRDLFGAFMNDPRLLPP
                     QYQQMARQDTPRAIADYIAGMTDRYAMKEHRRLFAVGEIH"
     gene            complement(19092..20171)
                     /gene="aroB"
                     /locus_tag="PKX01_08855"
     CDS             complement(19092..20171)
                     /gene="aroB"
                     /locus_tag="PKX01_08855"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HMZ76274.1"
                     /translation="MRTLNVELAARRYPIHIGTGLLDRSDLILPFLKTPRAAVVTNDV
                     VGPLYLARFSAALRAAGVRVTEIVLPDGEAHKDWPTLNLIFDGLLGERCDRATTVIAL
                     GGGVVGDMAGFAASAYQRGVPFIQVPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     RLVLADTSVLSTLPDRELRAGVAEVIKYGLIRDLPFLEWLEANLDRLLHRDGDVLAEA
                     IERSCRNKAEVVVADETEQGERALLNLGHTFGHAIETGLGYGEWLHGEAVAAGTVMAA
                     ELSRRLGWLSTADVTRVREIHARAGLPVRGAALGADRYLELMAHDKKVSNGTLRLVLL
                     KAIGRAVVTAEAPIDAVRASIDACC"
     gene            complement(20159..20686)
                     /locus_tag="PKX01_08860"
     CDS             complement(20159..20686)
                     /locus_tag="PKX01_08860"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMZ76275.1"
                     /translation="MTNPSRVVLVGMPGSGKTTVGRELAKRLGMRFLDTDHEIEVRTG
                     VKIPTIFEIEGEAGFRRRETQTLDALTGETGVVLATGGGAVIAAENRQMLAARGVVVY
                     LNVPPHILWERTRHDRTRPLLQVADPKARLQQLFAERDPFYREVAHIIVEGGRGTPAA
                     MVRTIEREIQELCAH"
     gene            complement(20742..23078)
                     /locus_tag="PKX01_08865"
     CDS             complement(20742..23078)
                     /locus_tag="PKX01_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76276.1"
                     /translation="MSIVDGTGTAITALPTSGSVTVQASVKNASGSAVANAVVTFATD
                     SAYGTLNPTTGTALTNSSGVAMIQLSAASLTAGGAATVTATATLDSVSVSGSANYTVS
                     AANYAVAVGIYDSTGGTATTTLPSSGSVTVKATVTNASGVAIPNINIKFTTDSALALF
                     NPSVGTAMTNSAGVASVQMSSAGLTASGAGQVTATITDATKPAVAGYASYQVGAANVT
                     LSAMNISATSIPAYGTSTVAVTVNVNGTPTTTPVTVAFSSGCATSGKASIASSVLTVN
                     GIATATYTDKGCNGTDTITATVLDKTVQGSITSAGPQPANIQFVSATPSSIALKGTAG
                     LIDISNVTFKVVDASGNAVPATTVEFYLTNWTGGIKLDDNTQTQVDALTGGKVRKQTA
                     ADGTVVVAVQAGTNPTSVWVLANVLNSSLSTQSSKLVISTGLPSQDRFSLAVGTHNIE
                     GWRYDGVTTPLTVYAFDRVGNPIPDGSTINFVTEGAGASPGTCSVGSGSCSTQFVSSE
                     SRPRNEGVGVCLNSSGAMVPSTNSGAVANTCAYSGRVAVLAYANGEESFDDANGNNLY
                     DSGENFRDVGDIYVDNNEDGVWESPEQYFPFAGSSGNTSTGAACSTTYSGYATAKSKA
                     NTCNGKWGQAQVRRDQVVVLSDSYVSNIYAITSNTTVSGGGVVVTDGFNFSSGAACGA
                     SYTIRIADLNNNPLPAGTTVAISNINVTYSQVGTTDPVTVDTDISGSIILDTIAPGGT
                     YHSITMLGKKCLTIPSGTMIIKTTSPKGLTTSIPVQVN"
BASE COUNT         3925 a         7347 c         7969 g         3935 t
ORIGIN      
        1 cggccgcctg acgcgcgcag cgcaggggag tcggccaaag gccgaccgcc ggaagtcggg
       61 tcgccttctt tgcccacttt cttggcgaag caagaaagtg ggtcgcccgc cggggcgaac
      121 tcccggccaa cggcgggctg aagtcccgac ccacgccaag ccaccccctt gaacacaaaa
      181 attcggacac ccccagcgcc tatcgcaccg cccctgcgca aaggattcac acgcctgtga
      241 atacgattcg cagccttgcg aattcgattc gcacccctgt gaactcgatt cacagccttg
      301 cgagaaggat tcacagcctc gtgaactcga ttcacacccc tgcgagaagg attcgcagac
      361 ccgtgaacac gattcacagc cctgcgagaa cgattcacgg ggcgatgaat cgtcctcgca
      421 acgggtgatt cgggccacgg cccgcggtgg attcagccca gcacgccgtg ggcgatgctc
      481 acgtagaggg ggatccccag caggatgttg aaggggaagg tgaggcccag ggacatgccc
      541 acgtacaggg acgggcgggc ttccgggatg gcgaagcgca gggcggcggg cacggcgatg
      601 taggaggcgc tggcggcgag caccatcagc agggcggcgt tgcccgcctc gatgccggcg
      661 agcaggcaca ggcccagggc gaggctggcg tggaccagcg gggcgagcag cgcgtaagcc
      721 ggcagccacg gggaggcgcc gcgcagctgg ggcagctggc gtgcggcgag gaggcccatg
      781 tccaggagga agaaggccag catgcccttg aagagatcgc cggagaaggg ctgcatggcg
      841 gccttgccgg cgtcgccggt gacgatgccg acgaccatcg cccccagcag gagcagttgc
      901 gcgccgtcgg tgagggattc gtgcagcacc ttgccgaagg acacgccgcc cggcgcaggg
      961 cccagggtgg cggtgccgtc cgcatgcacc tgcacgccgg cggtggcgct ctggcgcagg
     1021 tggttggcga ggagcacggc aaggacgatg gcgggcgatt ccatcagggc cagggcggcg
     1081 gccatgtgtc cgccgaaggg gtagccgtgg ctctccaggt attgggtggt ggtgatgaag
     1141 gtgacggcgc tcaccgagcc gtaggtggcg gcgatggcgg cggcgtcgaa tccgcccacc
     1201 ttgcggcgca gcacggtgaa gccgacgagg ggcacgatga cggccaggct caccgccacc
     1261 agcaggctgc tcgccacctg ggcggtgagg ccggaatggg ccagcgcaaa accgcccttg
     1321 aggcccagcg ccatgaggag atacagggac aggaagcggg agatggcggg cgggatttcc
     1381 agattggatt tgaggaggcc cgcggccacc ccgaagagga agaagaggat tgcgggatcg
     1441 agcagactgt tcatgatgag gctccgtaag gatctggatt catgttacgg cgcagcaatg
     1501 atcgagtaaa atagataata aatattgttt ttatcgacaa aactctatgt atgcgacgtt
     1561 tcggcagctt cagctctttc ttgcggtggc cgagcacggc agcatcagcg cggcggccaa
     1621 ggcctgccat gtgacccagc cgacggtgtc catgcagctg cgcgagttgg ccgacagcgt
     1681 cggcctgccc ctctacgagg tgatcggcaa gcgcgtcttc ctcaccgagg cggggcagct
     1741 ggtgcgccag tcggccctgg cgatccgcga cgagtgggcg gccctgggca cccgcgtgga
     1801 tgccctgcgc ggtttgcagc ggggctgcct gcgggtggcc ctggtgagca ccgcgaagta
     1861 ctttgtgccg accctgctgg gcgccttctg ccgccagtac ccggacgtgg aggtgagcct
     1921 ctccatcctc aaccgggacg gggtgctgga gcgcctgcag gccaacgcgg acgacctgtg
     1981 catcatgtcg ctgccgccgg acggggacga ggtgagccgc gaagcctttt tgcccaaccg
     2041 gctggtggtg gtggcccccg ccggacatcc cctggccgcc gaacaggcga ttgcgccggc
     2101 cgccctggca ggcgaacgct tcatcctgcg ggaaccgggc tcgggcacgc gacgcgcgtg
     2161 cgagcggcat tttgcgacgc tgggctggca gcccacggtg cgcatggccc tgggcagcaa
     2221 cgaggcgatc aagcattcgg tggcggcagg actgggactg gcagtgctgt cgcgccacac
     2281 gctgcacgcc gatccggcgg tggacgggct ggcgatcctg gacgtggcgg gctttccgat
     2341 tcacgccaac tggtacatcg tgcagccccg cggcaagcgt ctgtcgccca tcgccgcgga
     2401 gttccttcag catctgcggg aatatgtgga cagtctgccg gcgacgacgc gggcgtgagg
     2461 atcgccggcg cagcgcggcg gacaggcttt tcagggattc agggagctca ccatgctgga
     2521 actggcacgc attttcgggc tgttcgtcct caccgcgctg gcggagatca ccggctgcta
     2581 cctgccctgg ctggtgtgga cgcgcgggca caccccctgg ctgctgctgc cggcggcgct
     2641 gtccctcggc ctcttcgcgt ggctgctgac cctccacccc ggcgccgcgg gacgcatcta
     2701 cgcagcctac ggcggcgtgt atgtggcggt ggcgctgctg tggctgtggc aggtggacgg
     2761 agtgcgcccg agccgctggg actggatcgg cagcgcggtg tgcctggccg gcatggcgat
     2821 catcgcgctg cagccggggc gcgagggctg aggggcgcga cggccgcgac gctccgtcag
     2881 gctttgacca gacgcagcag ggtgatctcc gacggcgcgc cgaagcgttt cggaggcccc
     2941 cagtagcccg tgccgcggct ggtatagacc agcatccggc caaagcggtg caggccggcg
     3001 gtgaagggtt gttgcaaggg cacgaagtgg ttccacggcc agaactggcc gccgtgggtg
     3061 tggccggaga gctggagatg gaagccggca gcctccgccg cggccgcgga acggggctgg
     3121 tgggcgagca ggatgcgcag caggccgggc ggactgccgg ccagggccgc caccgggtcg
     3181 ctggcctggg cgggatcgaa gacgccggcg gagtaatccg tgacgccagc caacacgaag
     3241 cgggcgcccc ggtgggggat gagcacgtgg cggttgtgca ggccgcgcag gccgatgcgc
     3301 tcgaattcgc gcatccacgc ggcggcgccg gagtagtact cgtggttgcc ggtgaccatg
     3361 taagggccat ggcggctgcg cagtcgcccg aggggcgcgg tgtgggcgcg aagatggtcg
     3421 acgcggccgt ccaccaggtc gccggtgagg acgacgaggt ccgcatcgag cccattgacc
     3481 cggtccacga tggcctgcac gtagtcgccc ttgatggtgg ggcccacgtg gaggtcgctg
     3541 agctggacga gggtgaagcc cgccagcgca tcgggcagat cggccagggg gaggtccacg
     3601 cgccgcacac gggcgacgcg ccgggcgttg acgtagccga ccgcggtgac gaatgcggcg
     3661 agccccagca cgccgagggc cgtgggcgcg gcgagggcgg gatcgttcgc gatccacagc
     3721 aggccgtcgc gcagcacggt gagcaccagc agggtcgaga aggctcccat gagcagcccg
     3781 cccagcccgc tgagacggtc ggcccagccg ggatggggga tgaggaagcg cccgagcagg
     3841 cccaggggga tcaggccggc ggacaggacc agtccggcga tggcgagact cagcccgagg
     3901 gcgccgaggt cgagggcggg cagcagacgc cagcccaggt aggcgtgcag gccgcccagc
     3961 agcggcagga tgcgcagcag ggcgccgcgc agacgacggg gcaggcccgc gggctcgacg
     4021 gccggcggcg cttcggttcg ggtggaggac atggaagacc tgggacggtg ggcgaggcgg
     4081 ctttgccgcg gcgatgccgc cgcgcaaggc ccgtttggaa ggcgtgtcgg gtcaggccgg
     4141 atcggcggac ttgtcgccgg gctcggcgcg ggctgcggac gcgtcggccg cgtctgccga
     4201 tccgcagcag ccccagcggc gatggaagcg tcccttgaac tgttcccgtt cttccggcgt
     4261 gaggtgttcc cactgcgccc ggtgggcatg ccagcgcccg tggccgccgc cacgcaggcc
     4321 gccgaagagc aggcggctga gcaccagcac gcccagggcc tgccagtagc cgatgggcga
     4381 gacgccggcg aagagctggg gcagcagggc attccacaac agcatgacga cgccggtaac
     4441 ggcggcgacg cccgccacga cgagcaggcc gatcttgatg cagcgggcgg cgctgcgggg
     4501 gtggcgggag gtggagcagt tcatcatgat gatttccagt caggagtgat cgaggtaaag
     4561 aggcgccaga cgtgcgcgca ggtgcaggac ggcctggcgt ttccagccga gcagggtgtt
     4621 gaggggcacg ccgctttcgg cagccatgtc cttgaagctg cgcccatcga tttcgtgggc
     4681 gacgaagaca tcgcgcgggc cgggcgggag ttcgtcgagg gcgacggcga tggcgtcgag
     4741 ccactgacgc cgcagatgcg ccgcctccgg gccgccgtcg gtggtcggca gggcctgcgc
     4801 gagccagtgc tcggtatcgt cgtcgtcccc ctcgggcgcg agcggcagcg gctcttcgcg
     4861 acgcttgcgg aaacgatcca cgatgcggtg ccgcgccacc cggaacaacc aggcccccac
     4921 ctgctcgatg ggctccggca agcgccaggc ttcgaccagc tcgaagaaga catcctgcag
     4981 gatgtcctcc gcatcgcccg gatcgggcac ccgctgacgg atgaaacgcc ccagccgccc
     5041 gctttcacgg gagatggttt cagtcagatg tcgattccgc tcggccatcg gggcagtccc
     5101 ggatcgggac cggggcagtg aaaacgaaga gtccatgcca cggtagacga acgggagccg
     5161 tggatattgt ggggcggggg aaaaatagtt gaagcgcgac gccgggtgag ggggtacgcg
     5221 tggcggaatt gcttcacacg cgggatggcc ggggcctcgc ctcgcgtaca acgccccttc
     5281 gagaaacgac aaaggggcca gatggcccct tcgtttcctg ctgcgtccga caaaccttat
     5341 ttgcaaaggg cgaacgtcaa ttcctgcccc cgcttcactc caccgtcacc gacttcgcca
     5401 gattccgcgg cttgtctacg tccgtgccct tcaccagggc ggcgtggtag ctgaggagtt
     5461 gcagcgggat gacgtggagg atgggggaga gcatgccgta gtgttcgggc atgtgcagga
     5521 tgtgcacccc ttcggattcg ggaatctcgg agccggcgtc ggcgaagacg tagagttcgc
     5581 cgccgcgggc gcggacttcc tggagattgc tcttgagctt ttcgagcaat tcgtcggccg
     5641 gggcgacggc gatgacgggc atttccttgt ccaccagggc cagggggccg tgtttgagtt
     5701 cgccggcggc gtagctttcg gcgtggatgt aggagatttc cttgagcttg agggcgcctt
     5761 ccatggcgat gggccagtgg ctgccgcggc cgagaaacag ggcgtggtgc ttgccggcga
     5821 aacgctgggc ccacttttcg atttcgggtt cgagctccag caccttggcc acggccacgg
     5881 gcaggtggcg cagctgggtg aggtaaccgg attcctgctc ggcgccgagg cggccgttga
     5941 tcttggcgag ggtgagggcc agcagggcca gggcggcgag ctgggtggtg aaggccttgg
     6001 tggaggcgac accgatctcg gggccggcgc gggtgatgag gcgcagggcg gattcgcgga
     6061 cgatggcgga ctcgggcacg ttgcagatgg ccagggtctt ggtcatgccc aggcccttgg
     6121 cgtgcttgag ggcggccagg gtgtcggcgg tttcaccgga ctgggagatg acgacgacca
     6181 gggtgtcggg atcgggcacg gattcgcggt agcggtattc ggaggcgatc tcgacggtgc
     6241 aggggatcct ggcgacggat tcgagccagt agcgggcggt gaagccggag tggtagctgg
     6301 tgccgcaggc gaggatcagc acgcggcggg tggccttgag gacgtccacg gcctcggcac
     6361 cgaagatctg cgggctgatg gcgccggcgc cggcgatggg ttcgagcgtg ttggcgaggg
     6421 cctggggctg ctcgaagatc tccttctgca tgtagtggcg gtacttgccc agttcgacgg
     6481 cgtcggcgga caggctggag atgttgaccg ggcgctccac cggcgtgccg tcggcgaggg
     6541 tgacgcggta gccgtcgagg gtcagctcgg ccacgtcgcc gtcttcgagg tagacgacct
     6601 tgcgcgtgac ctggagcagc gcggcggtgt cggaggcggc gtagtggccg tcttcgccga
     6661 tgcccaggag caggggcgag cccttgcggg cgacgatgac gcggtcggga taggcttcgc
     6721 tgacgatacc gatggcgtag gcgccgacga gctgggcgat ggtgacgcgg accgcctcgt
     6781 agagatcgga gaccgtcttg agggtgtcgt gcaggaggtg ggcgatggcc tcggtgtcgg
     6841 tctcggaggt gaagacgtaa cccttggcct gcagcgcggc gcgcagttcc tcgaagtttt
     6901 cgatgatgcc gttatggacg acggccagcc cgccggagat gtgggggtgg gcgttgcgct
     6961 cggagggcac gccgtgggtg gcccagcggg tgtgggcgat gccgatggag gcgctcaggg
     7021 cggtggcatc ggcctgggcg gacagttcgg cgacgcggcc cacggcgcgc aggcgctgca
     7081 gggccggcgc accgtcggtc ttgagcacgg cgaggccggc ggaatcgtag ccgcgatatt
     7141 cgagcttgcg caggccttcg agcaggacgg ggacgatgtt gcgacgggcg atggcagtga
     7201 cgatgccgca catggtgtga atgcctttcg ttcaatgtga agacggcccg ctgcaggggc
     7261 cgccggaaat gtctaaacgg ttcaggactt cttggcggcc ttgaccgggc gggtccagcc
     7321 gggaacggtg acctgacgcg cgcgcgaaac cgtgagctgc tccgcgggtg catctttggt
     7381 cagcgtggtg ccagcgccca gggtggcgcc gcgggccacg cggacggggg cgacgagctg
     7441 ggtatcggag ccgatgaaga cgtcgtcctc gatgacggtg cggaatttgt tggcgccatc
     7501 gtagttgcag gtgatggtgc cggcgcccac attgacccgc tggccgatgt ccgcatcgcc
     7561 cacgtaggcg aggtgattgg ccttggagtg gtcggcgatc acgctgttct tgatctcgac
     7621 gaagttgccg acatgcacgt cttcgcccag gacggtgccg ggacgggtac gcgcgtaggg
     7681 gccgatgatg caggcaggcc ccatggtcgt gtcttcgatg tgggaatagg gcgcgaccct
     7741 ggtgccggcg ccgagggtgg cgttgcggag cacacagttg gcgccgatgc gcacgccgtc
     7801 ggcgagctcg acccggcctt cgaagacgca gttgacgtcg atctccacgt cgcggccgca
     7861 gatcagctcg ccacgcacgt cgatgcgagc ggggtcgatg acggtgacac cggcgtcggt
     7921 gagacgacgg gcgatggcgc gctggtggat gcgctcgagt tcggcgagct gggtcttgtt
     7981 gttgacgccg agggtttcct cgaaggcgtc gggctgggtg gtgacgacct cgaagccgtc
     8041 ggccacggcc atggcgatga tgtcggtaag gtaatactcg ccctgggcgt tgtcgttctt
     8101 cagccgggcg agccaggcgc gcaactgggt gaccggcgcg gcgaggatgc cggtgttcac
     8161 ttcacgcacc tgacgctgct cgggcgtcgc gtccttctct tcgacgatgg acgtcacctt
     8221 gccagcagcg tctcgcagga tgcgcccgta gccggtggga tcgtccatgc ccacagtgag
     8281 cagggccagc ttctcgggac cggtggcgcc caccaggcga cgcagggtgg cctcgccgat
     8341 caggggcacg tcgccgtaga ggatgagggc ggtgtcaccg tcatccacat ggggcatggc
     8401 ttgctgcact gcatggccgg tgccctgcgg cggatcctgg cgcgcccagg cgagatccgg
     8461 tgcatccagc gcctcacgca cgacttcgcc gccatggcca tacaccacga cgatgcgctc
     8521 ggcctggagt gcccgggccg tatcgagcac atgggcgagc aagggcttgg cggccagggg
     8581 ttgcaggact ttgggcagga tggagcgcat gcgcttgccc tgaccggcgg cgaggacgat
     8641 aaccttcatg gagtcttccg gatgcattcg gtatggaatg cgcgcgagtg tagcacggag
     8701 catttgcatc gatttgtggc ctgcaccacg agtgcgcaac ttgtcacaac agccctttgt
     8761 cgctgcgatg caacacgcca ctggcctaat actaaagttt ggtagcacac taccgtaagc
     8821 ttgtacttgc tgcgttgcaa aaccgagaga accaccatga acacccccaa tcacgacttc
     8881 atcgaaaacc ggaccttcga cgagatcgcg gtgggcgaca gcgcccatct cgtccgcacc
     8941 ctgcgcccgg aagacatcca tcttttcgcg gtcatgtccg gcgacgtgaa cccgacccat
     9001 gtggaccccg aattcgcccg ttccagccag ttccgcgagg tggtcggcca ctccatgtgg
     9061 ggcagcgtga tcatctcttc catcctcggc accgagtttc cggggccggg tacggtgtat
     9121 gtcggccaga cgctgaaatt ctggaggccc atcaccatcg gcgacacgct gaccgtcacc
     9181 atcacctgca cccaccgctt cgagcacaac caccacctgc tgctggattg ccagtgcgtg
     9241 aatcaggatg gcctcaaggt catcgacggc acggccgagg tgatggcccc caccgaaaaa
     9301 atcaagcgtc cccgcgtcgc cctgcctgag atcaccatct ccgaccgcca gcagcgctac
     9361 ctgcatgcgc tggaactcac caagggcctg gccccgatcc ccatcgccgt agcccaccct
     9421 tgcgacaccg aatccctgcg cggcccgctg caggcgcggg atgccggtct ggtgatcccg
     9481 gtcctggtcg gccccgaagc caagatccgt gaagtggccg agcgcgagca tctggatctc
     9541 aatggcgtac gcatcgtcaa cgtgccccac agccacgcct ccgccgagac cgccgtgcgc
     9601 ctcgcccgca ccggcgaggt cgaggcgctg atgaagggct ccctgcacac cgacgagctg
     9661 atgagcgaag tcgtcgccaa ggagaccggc ctgcgcacct cgcggcgcat ctcccacgtc
     9721 ttcatggccg acgtgcccac ctacccgcac ccgctgctca tcaccgacgc ggccatcaac
     9781 atcgaaccgt ccctggaaga caaggtgcac atcatccaga acgccatcga gctggccatc
     9841 atgatgggcg tgcccgagcc caaggtcgcc atcctctcag cggtcgaaac ggtgaacgtg
     9901 aagatccgct ccacccttga cgccgccgcc ctgtgcaaga tggccgaccg gggtcagatc
     9961 aagggcggtc tgctggacgg cccgctggcc ttcgacaacg ccgtctccct gatcgccgcc
    10021 aagaccaagg gcatccgctc cgccgtcgcc ggccaggccg acatcctggt ggtgcccgat
    10081 ctcgagtccg gcaacatggt cgccaagcag cttgaatacc tggccgacgc gctgatggcc
    10141 ggtgtggtgc tcggcgcccg cgtacccatc gtgctcacca gccgggccga caccgccgag
    10201 acgcgcaccg cgtcctgcgc catcgcccag ctcatggccc acaagcgtcg cgaaggaatg
    10261 cgcaaatgaa agccatcctg atcctcaacg ccggctcgtc cagcctcaag ttcgccctct
    10321 tcccgctggc cccgcggctc gccgacgcgc cgcgcctgtc cggccaggtg gaaggcatcg
    10381 gcgccgagcc gctgatgcac gccaaggaca cccactccgg cgaacgcttc accgaagccc
    10441 tgcccattgc ccccggcacc gaccagaaca cccagcatcg caacgcgctg gaattcgtct
    10501 tcgactggat ccgtcgccat agccccggcg tggacatcgt cgccgccggg caccgcatcg
    10561 tccatggcgg cgaccagtac ggcgcgcccg tggcgctgac gccggacgtg gtcggtgcac
    10621 tggagaccct gattcccctc gcgccgctgc accagcccca caacctgcgg gccatcaagt
    10681 cgctctttac gctgatgccc gatgtgcgcc aggtgggctg cttcgacacg gccttccatc
    10741 gcacccgcct gccggtggcc gagcgcttcc cgctgccccg cgcgctgttc gacgaaggtg
    10801 tcaagcgcta cggcttccac ggcctgtcct acgagttcgt gtcgcgccag ctccccgacc
    10861 tgctcggcga ggaaaaggcc cacggcccca tcgtcgtcgc ccacctgggt aacggcgcga
    10921 gcatgtgcgc cctgcgcgac ggactctccc gtgacaccag catgggcttc accgccgtgg
    10981 atggcctgat gatgggcacc cgtaccgggt ccctcgatcc gggcgtgatc ctgcacctca
    11041 tcgaacagaa aggcatggac gccaaggccc tgagcaatct ggtctacaag cagtccggcc
    11101 tgctcggcgt ctccggcatc agccaggata tgcgcgccct gctcgaatcc gacgagatcg
    11161 ccgcccagga ggccatcgaa ctgttctgct accgtgcggc ctgcatgatc ggccaactca
    11221 ccatggccgc cggcggtctg gatgcgctgg tgttcaccgg cggcatcggc gaacacgcgg
    11281 cgccgattcg cgcgcgcatt gccgaatggc tggcgtggac cggcctgcac ctctacccgg
    11341 ccgccaacca gcgccacgcc acgcgcatcc acgccaagga cagcagcgtg gaagtgctgg
    11401 tggtccccac caacgaagaa tggatgatcg cccaccacac ggtggaactg ctcggactcg
    11461 cctaagcccg aacggacgtc cagacatgaa aaggcccggt tcaagaccgg gccttttcac
    11521 tggggtcccc gacaccgtct aagtgccgga aaccccggaa gcgcccccgc atccgggggc
    11581 gaatcctcag cggggcagga cggaagaacc catcaggaag gtatccacct cccgggcggc
    11641 ctgacgacct tcgcggatcg cccacaccac cagtgactgg ccccgccgga cgtcaccggc
    11701 agcgaagacc ttgggcacgt tggtggcgta gcagccgacg ccatcggtgc tcgccttggc
    11761 gttgccgcgg ccgtccttgt cgacaccgaa ggcctccagc agggacgcca ccgggttggt
    11821 gaagcccatc gcgaaaagca ccagatccgc cggcagttcg cgcacggaac ctggcacttc
    11881 ggagggtttg ccgtccttcc actccagctg gacggtacgc agggccttga cctggccgtt
    11941 ctcaccgatg aactccttgg tggacacggc gaactcgcgg ttgcaccctt cgtcgtggga
    12001 cgaggacgtg cgcagcttga tcggccagta gggccaggtg agcggacggt tttcctcggc
    12061 cggcggctgg ggcatcacct cgaactgggt gaccgacagg gcgccgtgac gattggaggt
    12121 gccgacacag tcggaaccgg tatcgccgcc gccgatcacg atgacgtgct tgccggccgc
    12181 cgagatcgga ttcttgtctt cgccagcgac ttccttgttc tgcgggatca gcagctccag
    12241 cgcaaagtgg acgcccttca gctcgcggcc gggaaccggc aggtcgcggg ggacttcgga
    12301 gcccgcagca aggatcaccg cgtcgaactc gcgggtaagc tggtcagcac tgaccacctc
    12361 gacggaatcg tcgttgatgc cggtcaggcc aggcttggcc cccacgcaga cctgggtgcg
    12421 gaaggtcacg ccctccgcct ccatctgcgc cacgcgacgg tcgatgtggg acttctccat
    12481 cttgaagtcg ggaatgccgt agcgcagcag accgccgatg cggctgctct tctcgaacac
    12541 cgtcacatcg tgcccgacgc gggcgagctg ctgggccgcc gccagaccgg ccggaccgga
    12601 accgacgatg gcgaccttct tgccggtctt gaccgcggcc ggctggggct tgatccagcc
    12661 gctttcccag gccttgtcga tgatggcgtg ctcgatggac ttgatgccca ccgcatcggt
    12721 gttgatgttg agggtgcacg ccgcctcaca gggcgccgga cagatgcggc cggtgaagtc
    12781 cggaaagttg ttggtggaat gcagcacctc gatggcctgc tcccactgac cgcggaacac
    12841 cagatcattc cagtcgggaa tgatgttgtt caccgggcag ccgttgttgc agaaggggat
    12901 gccgcagtcc atgcagcgcg cgccctgtcg ggccgcttca tcgtccttca gcgtacagac
    12961 gaactccttg tagtgcttga gtcgctcctc gaccggagca taggcctcgg agaggcgctg
    13021 gaactcgaga aatccagtgg gcttgcccat gttaagcggc ctccagttct tgctgtttct
    13081 gcgcggccat ttcggccagt gcgcgacggt actcgtgcgg catcaccttg acgaacttgg
    13141 cgcggtattc gctccagtcc gccaggatgg ccttggcgcg ttcgctgccg gtgtagcggg
    13201 cgtggttctc gaccagctgc ttgagcagtg cttcgtcggc catgcccagg tgacggacat
    13261 ccacgcgacc atgcgcttcc agctcgtcgc cggactcgga accctgacgg gcggccagtt
    13321 cctcttccac cggctccagg gccacctggg ccatgttgca gcgggattcg aaggtgccat
    13381 ccgtgtcgag cacgtaggcc acgccaccgg acatgcctgc ggcaaagttg cggccggtca
    13441 tgcccagcac caccacggtg ccaccggtca tgtattcgca gccgtgatcg cccacgcctt
    13501 ccaccaccgc ggtcgcgcct gagttgcgca ccgcgaagcg ctcgccgccc acgccggcga
    13561 agtacgcttc gccctcggtg gcgccgtaca tgacggtgtt gccgatgatg atgttgctcg
    13621 gggtctcacc gcggaagccc gccgccggac gcacgacgat gcggccgccg gagaggccct
    13681 tgcccacata gtcgttgccc tcgcccacca gatcgagcgt gacgccacgc gccaggaagg
    13741 caccgaagct ctgaccggcg gtaccgttca ggcggatgtg gatggtgtcg tcgggcaggc
    13801 cggcgtgacc gtacttggcc gcgacgcggc ccgacagcat ggcgccgacg gtgcggttga
    13861 tgttgcgcac gggcagttcg atgttgaccg cctcggcctt ttccagcgcc ggcttggcca
    13921 gctcgatgag ctgattgtcc agagccttgg ccaggttgtg atcttgggtt tcggtatgca
    13981 ggcggggcac tgcggccggc acattgggca gatggaagat gcggctgtaa tccaggccgc
    14041 gggccttcca gtggtcgatg ccggcacgca tgtcgagcag gtcggcgcgg ccgatcagct
    14101 cgtcgaactt gcggatgccc agctgggcca tgatctcgcg cacttcctcg gcgatgaaga
    14161 agaagtaatt caccacgtat tcgggctgac cggagaagcg tttgcgcagg gtcggatcct
    14221 gcgtcgcgac acccaccgga caggtgttga ggtggcactt gcgcatcatg atgcagcctt
    14281 ccaccaccag cggcgcggtg gcgaagccga actcgtcggc ccccagcagg gcacccacga
    14341 ccacgtcacg gccggtcttg atctgaccgt ccacctgcac gcggatgcgg ctgcgcaggc
    14401 ggttgagcac cagggtctgc tgggtctcgg cgagaccgag ttcccacggg gagccggcgt
    14461 acttgatgga cgaccagggc gaagcgcctg tgccgccatc gtgaccggcg atcaccacgt
    14521 ggtcggcctt ggccttggcc acgccggccg ccacggtccc gacgcccact tcggacacca
    14581 gcttgacgga gatgcccgcc gccggattgg cgttcttgag atcgtggatc aactgggcca
    14641 gatcctcgat ggagtagatg tcgtggtgcg gcggcggcga gatcaggccg acgcccggca
    14701 ccgagtgacg caggaagccg atgtactcgg acaccttgtg gccgggcagc tggccgcctt
    14761 caccgggctt ggcgccctgg gccatcttga tctggatctg gtcggcattg accaggtatt
    14821 cggtggtcac gccgaaacga cccgacgcca cctgcttgat cgaggagcgc aggctgtcgc
    14881 cggccttgag ttcgatgtcg cgctcgatgc ggctggcacc gatgacttca gacagcttgg
    14941 tcggctcggc gatggggatg aagcgcttcg gatcctcgcc accttcgccg gtattggagc
    15001 gaccgccgat acggttcatg gcgatggcca gggtggtgtg ggcttcggtg gagatggagc
    15061 ccagggacat cgcacccgtg gcgaaacgct tgacgatgtc cttggcggac tcgacttctt
    15121 ccagcggcac cggggcgccc gccggcttga actcgaacag gccgcgcagg gtcatgtggc
    15181 gcttgctctg atcgttgatg atgcgggcgt actccttgta cgtgtcgtac ttgccggagc
    15241 gggtggagtg ctgcagcttg gcgatggcgt ccggggtcca catgtgctct tcgccgcgga
    15301 tccggaaggc gtattcaccg ccggcgtcga gcatgccggc cagcaccggg tcggcgctga
    15361 aggcctgctg gtggaggcgg atggcctcat ccatgacctc gaacacgccg atgccttcca
    15421 cctgggacga ggtacccgtg aagtactttt ccaccagcga cttctgcaga ccaaccgctt
    15481 cgaagatctg cgcgccggtg taggacatga aggtggaaat gcccatcttg gacatgacct
    15541 tcatgaggcc cttgccgaca cccttgatga agtgcttgac gtacttttct gcggtctcgg
    15601 cgtccccggc catctgactg agggtttcca gagccaggta ggggtggacc gcttcggcgc
    15661 catagccggc gagcacggcg aagtgatggg tttcccgggc ggagccggtt tccaccacca
    15721 ggccggtgcg ggtacgcagc cccttggcca ccaggtgctg atgcacggcc gaggtggcga
    15781 gcagcgccgg aatggccaca ttgagcgcgt ccaccttccg gtcggaaacg atcaggatgt
    15841 tggagccgtt gagcaccgcg tcctcggctt ccgcacagag cgaggcgaga cgtgcctcga
    15901 cgccttcctt gccccaggcg atcggatagc agatgtccag ttcggcggag cggaacttgt
    15961 cttcggtgta gcgcgcaatc tcccgcagct tggccatgtc ctcgacggtc aacaccggct
    16021 ggggcacttc gaggcggtag ggcgggttga tctcgttgat gccgagcagg ttgggacgcg
    16081 gcccgacgaa ggtcaccagg gacatgacca actgctcgcg gatcgggtcg atcggcggat
    16141 tggtcacctg ggcgaagagc tgccggaagt acatatacag caccttcgga cgggccgaca
    16201 gcacggccag cgggctgtcg ttacccatcg aaccggtacc ttcctcgcca ctcttggcca
    16261 tcggctcgag gatgaacttg atgtcttcct gggagtagcc aaaggcttgc tgacgatcaa
    16321 gcagggaggc cgcgtattcc ggtgcggcgg aaccggccgg atccgggcag gcgtcgaggc
    16381 ggatgttgat gcggcggatc cactcctggt agggcttggc ggcggccagg gtgtccttga
    16441 gttccttgtc gtcgatgatg cggccctgct ccatgtcgat caggaacatc ttgccgggct
    16501 gcaggcgcca cttcttgacg atcttttcgt ccgggatggg gagcacaccg gactctgacg
    16561 ccatcaccac gtaatcgtcg tcggtcacca ggtagcgggc gggacgcagg ccgttacggt
    16621 ccagcgtggc accgatctgg cggccgtcgg tgaaggcgac ggcggcaggg ccatcccagg
    16681 gctccatcat ggcagcgtgg tactcgtaga aggcacgacg cttggcgtcc atgagggtat
    16741 gggattccca ggcttcggga atcatcatca tcatggcatg ggccagggaa tagccgctca
    16801 tcacgagcag ttcgagggcg ttgtcgaagg acgcggaatc ggactggccg ggatagatca
    16861 gcggccacag cttgtagaga tcgtccccca gcagcggcga atgggtgccc ttttcgcggg
    16921 cgcgcatcca gttgtagttg ccgcgcaccg tgttgatctc gccgttgtgg gcgatgtagc
    16981 ggaacgggtg ggccagatgc caggtcggga aggtgttcgt ggagaagcgc tggtgcacca
    17041 gggccaacgc ggaatcgcag cgcgcgtcct ggaggtcgag gtagtactgg ccgacctgat
    17101 cggcgaggag cagacccttg tagttgatgg tccgcgccga catggacggc gtgtagaact
    17161 ccttgctgtg cttgaggcgc agactgccga tggcgttggc cgcacggcgg cggatcacat
    17221 acagcttgcg ctcaagcgct tcggtgacca tcacgtcggg accgcgaccg atgaagacct
    17281 ggcggatcac cggctccttg gccttgacgg tgggcgacat gggcatcgcg ggatcgatgg
    17341 gcacgtcacg ccagcccagc acgacctgac cttcggcgcg ggcggcacgc tcgatctcct
    17401 cctcgcaggc gagacgggac gcgtgctcct tgggcaggaa caccatgccc acgccgtaat
    17461 cacccggcgg cggcagggtg acgccctgct tggccatctc ctcgcggaac aggagatcgg
    17521 gaatctggat caggatgccg gcaccgtcac cctgcagggg atcggcaccc acggcacccc
    17581 gatggtccag attcttgagg atttcgagac cctgggtcac gacatcgtga gacttcttgc
    17641 ccttgatatg tgcaatgaaa ccgacaccac aggcgtcatg ttcgttggcg gggtcgtaga
    17701 gtccctgctg cttgggaaga tccatcttgt ttttcctcga cacaacacgc accggccccc
    17761 tccatcggag gtttaccggc cttacaaact gcggaaaatg ccccgtccga accgtgaaaa
    17821 agccggcggg cgcaagcatg ccggcttcga gagatcccga cgaggagacc gttcaaacac
    17881 attgggcgag ggtcgaatat accgccgcag ggccttgtgg ttcaaggatt ttttgcagcg
    17941 cgaaaaaacc gtgcggcgct caatgaatct cgccaacggc gaagaggcgc cggtgttcct
    18001 tcatggcata gcggtcagtc attccggcga tgtagtcggc gatcgcccgc ggcgtatcct
    18061 ggcgcgccat ctgctgatac tggggcggca gcaggcgcgg atcgttcatg aaggcgccaa
    18121 agagatcgcg gatgatgcgc cgcgccttgt cggtcatgcg cagcacctga tagtgccagt
    18181 agaggttttc ccgcagaaag gtcttgagga cgcgcagatc cggcaacaga tcggcactga
    18241 aggccagcag cctgggcgcc gaggccacgt cgccggtgct cgcgacgccg gacgcagcaa
    18301 tgttggtggt cgactggcgg atcaggtcca cggccatcag gttgatcatg cggcggatcg
    18361 tctcatggac caggcggcgc cccgagagcc ccggatattc ttgttcgacg gaccgccggt
    18421 tgcgcgcaaa gacgggcaca tcgtcgagtt gctcgagggt gatcagaccc gagcgcaatc
    18481 catcgtccac gtcgtgattg ttgtaggcga tctcatccgc cagattggcg agctgggcct
    18541 ccagcgaggg ctgacgtcct tccaggaagc gccggccaac gtcaccgagc aactcggcgt
    18601 tcttgggtga acagtgcttg aggatgcctt cccgcgtttc aaacatcagg ttgagaccgt
    18661 cgaaggcggc gtagctctcc tccagcacgt ctaccgttcg cagggactgg aggttgtgct
    18721 cgaacccccc gtagtccttc atgcaggcgt tgagtgcctc ctggccagcg tggccgaagg
    18781 gcgtgtggcc gaggtcgtgg gccagcgcga ccgcctcggc gaggtcctcg ttgagatgca
    18841 gcgcacgggc gatggtccgg gtgatctgcg cgacctcgat gctgtgggtg agccgcgtgc
    18901 ggaagaggtc gccttcgtga ttgacgaaga cctgggtctt gtattcaagc cggcggaaag
    18961 ccgttgaatg gacgatgcgg tcccgatcgc gctgaaactc gccccgtgca gcgggcgggg
    19021 cttctggaat cctgcggccc cgggaaaccg cttcggtgac ggcgtagggg gcgagcacac
    19081 tcatcgcggc ttcagcagca ggcgtcgatg ctggcgcgga cggcatctat cggcgcttcg
    19141 gccgtcacca cggcacggcc gatcgctttg aggaggacga gccggagggt gccattactg
    19201 accttcttgt cgtgcgccat gagttcgaga tagcggtcag cccccagcgc cgcaccccgc
    19261 acgggcaggc cggcccgggc gtggatctcg cgcacacggg tcacatcggc cgtcgacagc
    19321 caacccagcc ggcgtgacag ttcggcagcc atgacggtcc cggcggcgac cgcctcacca
    19381 tgcagccatt ccccgtaacc gaggccggtc tcgatggcgt ggccgaacgt gtgaccgaga
    19441 ttgagaaggg cccgctcacc ctgctcggtt tcgtcggcga caacgacttc ggccttattg
    19501 cggcacgaac gctcgatggc ttcggccagc acatccccgt cgcggtgcag cagccggtcg
    19561 agattcgcct ccagccactc gagaaaaggc aggtcgcgga tcaagccata cttgatgact
    19621 tcggcgacgc cggcgcgcag ttcccgatcc ggcagcgtgg acagcaccga ggtatcggcc
    19681 aggaccagcc gcggctggta aaaggcaccg atcatgttct tgccaagcgg gtggttgatg
    19741 gcagtcttgc cacccaccga ggaatccacc tgggacagca gcgtagtcgg cacctggatg
    19801 aagggcacac cccgctggta ggccgatgca gcgaagccgg ccatatcgcc gacgactccg
    19861 ccaccaaggg cgatgacggt tgtggcgcga tcgcatcgct cacccagcag accgtcgaag
    19921 atcaggttga gtgtcggcca atccttgtgc gcttcaccat cgggcaggac gatttcggtc
    19981 acccggacgc cggccgcacg cagggcggca ctgaaacgtg cgagatagag cggcccgacc
    20041 acatcgttgg tgacgaccgc agcccgtggg gtcttgagaa agggaaggat cagatcgctg
    20101 cgatcaagca agccggtgcc gatgtgaatc ggatagcggc gcgctgccag ttcgacgttc
    20161 agtgtgcgca tagttcctgg atttcgcgct cgatggtgcg caccatggca gcgggggtcc
    20221 cccgcccgcc ctcgacgatg atgtgcgcca cctctcgata aaagggatcc cgctcggcga
    20281 acagctgctg caagcgcgcc tttgggtcgg ccacctgcag cagcggccgc gtacggtcgt
    20341 ggcgggtgcg ctcccaaagg atgtggggcg gcacattcag gtaaacgacg acaccgcggg
    20401 ccgcaagcat ctgacggttt tctgctgcga tcacggcgcc tccgccagtc gccagcacga
    20461 cgcccgtttc gcccgtgagg gcgtcgaggg tctgggtctc ccggcgccgg aacccggctt
    20521 caccctcgat ctcgaaaatg gttggaatct tgacgcccgt gcgcacctcg atctcgtggt
    20581 cggtatcgag gaagcgcatg ccgagacgct tggccagttc ccgaccaacc gtcgtcttgc
    20641 ctgaacccgg catgcccacc aggacgactc gggaaggatt cgtcatcctg aggctccggg
    20701 tccgtgcgcg ccagccagga cccgttctgc ggaaccagga atcaattcac ctgcaccgga
    20761 atggacgtag tcagcccctt cgggctggtg gtcttgatga tcatcgtccc ggacggaatc
    20821 gtcaggcact tcttgcccag catcgtgatg gaatgatagg tcccgcccgg tgcgatggtg
    20881 tccagaatga tcgagccgct gatgtcggta tccacggtca ccggatcggt cgtccccacc
    20941 tgcgagtagg tcacattgat gttggagatg gccacggtcg tccctgcagg cagcggattg
    21001 ttgttcagat cggcgatccg gatcgtgtag ctcgccccgc aggcggcccc cgaggagaaa
    21061 ttgaagccgt cggtcaccac aacgcccccg ccggaaacgg tcgtgttgct cgtaatcgca
    21121 tagatgttgg acacataact gtcggacagc acgacgactt ggtcgcgccg cacctgcgcc
    21181 tgcccccact tgccgttaca tgtattggcc ttggatttgg cggttgcata tcccgagtag
    21241 gtcgtcgaac aagcggcgcc agtgctcgtg ttgccggagg agccggcaaa cgggaagtac
    21301 tgctccggag attcccacac gccgtcttcg ttgttatcga cgtagatgtc gccgacgtcc
    21361 cggaagtttt caccactgtc gtagaggttg ttgccgttcg cgtcgtcgaa ggactcttcg
    21421 ccgttagcgt aggccagcac ggcaacccga ccggaatacg cacaggtatt ggcaaccgcg
    21481 ccactgttcg tgctcggcac catcgcgccc gacgagttga gacacacgcc gacgccttcg
    21541 ttacgcggac ggctctcgct actgacaaac tgggttgagc aggagccgct ccccaccgaa
    21601 cacgtgcccg gcgacgcgcc cgccccctcg gtgacaaagt tgatggttga accatcggga
    21661 atcggattgc ccacccggtc aaacgcatac acggtcaggg gcgtcgtcac gccgtcgtag
    21721 cgccagcctt cgatattgtg cgtgcccaca gcgagggaga agcggtcctg ggacggcagg
    21781 ccagtcgaga tcaccagctt gctggattga gtgctcaggc ttgaattgag gacattggca
    21841 agcacccaca ccgacgtcgg attggtaccc gcctgcaccg cgacgacaac cgtcccgtcc
    21901 gcagcggtct gtttgcggac cttaccgccg gtcagggcgt ccacctgggt ctgggtgttg
    21961 tcgtccagct tgatgccgcc ggtccagttg gtgagataga actcaactgt cgttgccggc
    22021 accgcattgc ccgacgcatc cacgaccttg aaggtcacgt tggagatgtc gatcagaccg
    22081 gcagtcccct tgagcgcaat ggacgacggt gtcgctgaca cgaactggat gttggcgggc
    22141 tgaggcccgg ccgaggtgat ggagccctgg acagtcttgt ccagcacggt ggccgtgatg
    22201 gtatccgtcc cattgcagcc cttgtcggta taggttgccg ttgcgatgcc attcacggtc
    22261 aggacactgg atgcgatgga cgccttacca ctggttgcgc agccggacga aaacgccacg
    22321 gtcaccggcg tggtggtcgg cgtgccgttg acgttgacgg ttaccgcaac cgtgctcgtc
    22381 ccgtaggccg ggatgctggt ggcgctgatg ttcatggcac tgagcgtcac attggccgcg
    22441 ccgacctgat acgaggcata gccggcaacg gcgggcttcg tggcatccgt gatcgtcgcg
    22501 gtcacctgcc cggcgccgga tgccgtcagc ccggccgaac tcatttgcac gctggccacc
    22561 ccggcgctgt tggtcatggc cgtacccacg ctcggattga acagcgcgag cgcactgtcc
    22621 gtagtgaact tgatgttgat attcggtatc gcgacccccg aagcattggt caccgtcgcc
    22681 ttgacagtca ccgatccgga cgatggcagc gtggtcgtcg ccgttccgcc cgtgctgtcg
    22741 tagataccca ctgcgaccgc atagttcgcg gcagacaccg tgtagttcgc cgaaccggac
    22801 acgctaaccg aatccaaggt ggctgtggcc gtgacggtcg cggcgcctcc cgcggtgaga
    22861 ctcgccgcgc tcagctggat catcgccaca ccactgctgt tggtcagcgc cgtacccgtc
    22921 gtcggattca gtgttccgta ggcactgtcg gtggcaaatg tgacgaccgc gttcgccact
    22981 gcgctcccgc tggcattctt gacgcttgcc tgaaccgtca ccgacccgct ggtcggcaag
    23041 gccgtaatcg ccgtgccggt cccatcaacg atgctcacgg tcaccgttcc acccgtagag
    23101 ccagaactgc ccgaactgga agaacacccg atgaatccgc atccgccgcc gccgccaccg
    23161 cctccacaac cggcga
//
LOCUS       DAOQPA010000051        23104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1058_length_23104_cov_17.706408, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000051 DAOQPA010000000
VERSION     DAOQPA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1058_length_23104_cov_17.706408"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PKX01_08870"
     CDS             complement(<1..1165)
                     /locus_tag="PKX01_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HMZ76277.1"
                     /translation="MNIPSLDFHLGETLTALRDTVQAFAAKEIAPRAAAVDRDNLFPA
                     DLWKKLGDLGLHGMTVEEDYGGSAMGYLAHIIALEEVSRASASVGLSYGAHSNLCINQ
                     IRRNGSEAQKRKYLPPLIAGDHVGALAMSEPNAGSDVVSMKLRAERKGERFVLNGSKM
                     WITNGGDADTLVVYAKTDPDAGPRGMTAFIVEKGMQGFSHGSHLDKLGMRGSNTFPLF
                     FDDVEVPEENVLGGVGNGARVLMSGLDYERAVLCGGPLGIMAACMDLVVPYLHERKQF
                     GQAIGEFQLMQGKVADLYTTWMATRAYVYAVGQACDRGDHARSLRKDAAGAILYSAEK
                     ATWMAGETIQTLGGVGYTNDYAAGRLWRDAKLYEIGAGTSEIRRMLIGRELFAE"
     gene            complement(1209..1679)
                     /locus_tag="PKX01_08875"
     CDS             complement(1209..1679)
                     /locus_tag="PKX01_08875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ76278.1"
                     /translation="MHRPLPESFIPRDPAYAGRVRASFARQRAMALIGATLERVEPGC
                     TEIHLPTRDDLTQQHGFIHGGVIGMVADSAAGYAAATLVAADAEVLTVEYKLNLVAPA
                     EGECLVARGEVVKSGRSLSITRADVFAVRAGAWRLCAVMQQTIMNLHARPGPSS"
     gene            complement(1776..2213)
                     /locus_tag="PKX01_08880"
     CDS             complement(1776..2213)
                     /locus_tag="PKX01_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMZ76279.1"
                     /translation="MSQENTYTIAELAREFGITPRAIRFYEDEGLLSPGRVGRNRVYS
                     KRDRTRLRLILRGKRLGLSLAEIGELIGMYDGVRNSKPQLARFLVVLAERRAAIEQQR
                     EDIVAVLAEIGMLETQCRELLAENPAAAAEARAELAQQMDKNN"
     gene            2328..3359
                     /locus_tag="PKX01_08885"
     CDS             2328..3359
                     /locus_tag="PKX01_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ76280.1"
                     /translation="MTQAPDAQLDYPCGEPPAPGDTREIAPGVHWIRMPLPFALDHIN
                     LWLIEDGDGLTLVDTGYGLEVTRDAWETILTRFARPVTRIVVTHCHPDHLGLAQWLAE
                     RSGAPVHLTLGEFLAAHALWHQLPGYNVSSMVAQFRRNGLDDARCAALEGRGNAYRKG
                     IPSLPESYCRLLHDDVLDIGGRAWRVISGYGHAPEHASLYCAEAGVLISGDMLLPRIS
                     TNVSVFAATPDDDPLARFLDSISAFKHLPGATLVLPSHGSPFRNIGLRVDQLVQHHAA
                     RCADLLGACDTPRSAGELLTTLFPRELDTHQVMFAMGEAIAHLNHLAHKREVCRVSGE
                     NGVVRFVKI"
     gene            3382..5202
                     /gene="phaC"
                     /locus_tag="PKX01_08890"
     CDS             3382..5202
                     /gene="phaC"
                     /locus_tag="PKX01_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HMZ76281.1"
                     /translation="MAAPEKEVTELPDPKEIAKTYAEVAQRASKLLADHMQKQVKKGI
                     ATPSDELGIAQAFMDMMGKLLANPYRLAQAQMNLVWDYFSLWQHSMMRFTGMGGGAPV
                     ATPAKDDKRFKDEEWQEHFLFDFIKQSYLITARHIHDTVSTVDGLDDQSQKKVNFYTR
                     QYIDALSPSNFALTNPEVFRETVKSHGQNLIKGLNNLLRDVEDGGGNLRVRMTDTSAF
                     ELGRNVATTPGKVVFQNEMMQLLQYTPSTPEVSKRPLLIVPPWINKFYILDLREKNSY
                     IKWCVDQGHTVFVISWVNPDERLAEKSFDSYVLEGTMAALDAIAEQTGAKEINAAGYC
                     LGGTLLATTLAYCAAKKDKRLASGTFFTTMTDFADPGELSVFIDEGQITSLEKKMFER
                     GYLEGSEMAGTFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNSDSTRMPAKMHSFY
                     LRNMYLQNLLKEPGGIEIGGLPIDLGKIKVPTYFISTIEDHIAPWKSTYSGARNFGGN
                     VRFVLGGSGHIAGIVNPPAANKYGYWINTAAKMPATADDFFAGAEQHPGSWWTDWQAW
                     LMDQDSSKVPARDPASGKLTVLEDAPGSYVKARIELKAKA"
     gene            5312..5818
                     /locus_tag="PKX01_08895"
     CDS             5312..5818
                     /locus_tag="PKX01_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ76282.1"
                     /translation="MSAHILPDPLPAGLPVVLDTNTVLALWMFEDPRLPLLRGAVESG
                     HLRPFTRQDCLDELQRVLAYAQFGQPPERQAALAATYATCCARIEPVAEPEVVLPACL
                     DRDDQKFLETARDAPARLLVTRDKLLLKLARRAVIAARFAILTPERIEALLARQGMAP
                     SPAPQSLT"
     gene            5882..6700
                     /locus_tag="PKX01_08900"
     CDS             5882..6700
                     /locus_tag="PKX01_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ76283.1"
                     /translation="MPIDSSLDVSKLVYTYLIPLGWKIAGAVALWIIGGWVINVIGNL
                     SSRGMDRQKVEPTLSRYVEATLRVIMRIVLVIIILSVFGIETTSFAALLAAAGVAIGA
                     AWSGLLANFAAGAFLIILRPFKVGDFISGGGVTGTVREIGLFATTVDQPDNVRTTIGN
                     NKLFADNIVNYTSNPFRRVELTAQIAHSVDPFEAIELIRRRIAEIPNVLASPAPDVEI
                     LEFNAAGTKLAVRPHCHNDHYWQVYFDTNKALAEVGGAHGWPIPAPHQVLRQPS"
     gene            complement(6793..7623)
                     /locus_tag="PKX01_08905"
     CDS             complement(6793..7623)
                     /locus_tag="PKX01_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMZ76284.1"
                     /translation="MRDAIVNPLQDALGICHRPAARDARIVCLVPSITELVWDLGLGA
                     QLVGRTGFCVRPREVLRAVAKVGGTKDVNLEKLRALAPSHVIVNVDENRRETVEALAG
                     FVPHVIVTHPCAPQDNFALYRLLGGIFGRVAEAEALCAGLADALAQAYAAAADLPRER
                     VLYLIWRDPWMTVSAGTYIAATLAAVGWDAVAVASPARYPAFDWAEPAVQAAARVLLS
                     SEPYRFQLKHEAEVTALSGRPARRLDGEWASWYGSRAIAGLRALVRLRRSLHEGVAGA
                     "
     gene            7768..8883
                     /gene="ald"
                     /locus_tag="PKX01_08910"
     CDS             7768..8883
                     /gene="ald"
                     /locus_tag="PKX01_08910"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ76285.1"
                     /translation="MKIGIPRESKNHEYRVGLTPGAVREALAHGHDVIVETGAGHGIG
                     ASDSDYERAGARIALNVDEVFGEVEMIVKVKEPLAAERARLRPHHLLFTYLHLAPDPG
                     QAEDLLASGATAIAYETVTAPGGGLPLLAPMSEVAGRMSIQAGAAALEKSHGGRGVLL
                     GGVPGVEPGRVVVIGGGTVGNAAARTALGMGADVTVLDRSLAVLRHLDDVYRGHLKTI
                     HATRDAIERHVLQADLVIGAVLIPGAAAPKLVSRAMLNAMKPGAVIVDVAIDQGGCFE
                     TSRPTTHADPTYVVDGVVHYCVANMPGAVARTATYALNAATLPYVLALADKGWKQALK
                     DDVHLRAGLNVCAGLVTHPQVAEALGKKYVPAITLLG"
     gene            8970..9440
                     /locus_tag="PKX01_08915"
     CDS             8970..9440
                     /locus_tag="PKX01_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ76286.1"
                     /translation="MNPSPLYTVEQYAQEDSIGFLFHQAKLRLTQAMDERIGDLGITA
                     AQWVVLKQIALRSGETASALCKCSGCDTGSMTRMLDRLEEKGLIRRERSTADRRVVQL
                     HVTDAGQALLPAIVPRVVDVLNTAVVDFSREELDMLKGLMRRVVANLDARAPQD"
     gene            9570..10982
                     /locus_tag="PKX01_08920"
     CDS             9570..10982
                     /locus_tag="PKX01_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ76287.1"
                     /translation="MTLLLGACVSPAGLAPRGTPTDPATLAATRSLADTPAGDWPATT
                     WWTAFGDPQLDRLVAAALQGSPTLDLARARIDRARAQAGVAAAVSDVHVGANVDGAQQ
                     RLSEHYIFPSGLGGTYTSINRAALDFSLDLDFWGRNRAALAAAVGEARATEADAAEAR
                     LVLASAVVRSYLQFDLLHRLRAIADATVTQREHLQKLVHTRQQAGLDARAEVQQAIGS
                     VSAAKAERSALDTQLEQVRHQLAALTGQGPDAALDFTPPQVHAARVALPEVLPADLLG
                     RRPDVVAQRWRVEAARQGSAAARAEFYPNVNLSAFVGVQSLGLHPLFESGSRIVGIGP
                     AIHLPVFDSSRLRANLAVRHGEEDIAIAQYNATLVDALRDVADQLSVWRGAHAQARDQ
                     DAALARYEEAWRLATVRYRAGLTSYTAVLAAETPLLAQRRLSAELHNRQLDAGAGLAR
                     ALGGGLALVTPPTPTTSTLR"
     gene            11006..12259
                     /locus_tag="PKX01_08925"
     CDS             11006..12259
                     /locus_tag="PKX01_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ76288.1"
                     /translation="MTAPTPDSTPNTARRRSLLAIATACGLAGLAWGGWWFTHGRFLE
                     QTDDAYVAGNVIQITPQVAGTVIAIDADDTQTVRAGQTLVRLDPADARVALEQAEAQL
                     GQSVREVQALYAGGGALEATVRQREADLARARDDLQRRQQVAGSGAVAEEEIEHARAA
                     LRAAETALQTAREQLVTNQVQTRGTQIATHPNVQRAAARVEESYLAYARSAVPAPVGG
                     LVARRSVQVGQRVAAGAPLMAVVPLDQVWVDANFKESQLHGMHAGQPVTLAADVYGSR
                     VTYHGHIAGLAAGTGAAFALLPAQNASGNWIKVVQRVPVRIALDPRELAAHPLRIGLS
                     MQVEVDLRDSTDSDAPLAQAVPASPVAATDVYAELGKAARARIQAIIGANSGGPAAPP
                     LAEPVTPGTTPAAAAQATAPRRTQA"
     gene            12264..13811
                     /locus_tag="PKX01_08930"
     CDS             12264..13811
                     /locus_tag="PKX01_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HMZ76289.1"
                     /translation="MAGPIALPPLEPGARRLATVALSLATFMNVLDTTIANVSLPNIA
                     GDLGVSPSQSTWVITSFGVANAIAVPLTGWLSQRFGQVRLFVTSVVLFVIASWLCGLA
                     HSLEMLIAARVLQGAVAGPMIPLSQALLLQSYPREKSGMALAMWSMTTLVAPVMGPIL
                     GGWLSDNLSWPWIFYINLPVGLGAAWMSWQLLRERETPTRKLPIDRVGLVLLVVWVGA
                     LQIMLDKGKELDWFESGEIVALGAVALVGFALFVAWELTEAHPVVDLTLFARPNFLFG
                     VIALAAGYGVFFGNVVVMPLWLQQFMGYTATWAGLVTAPIGLLALLLSPFIGRIMPRT
                     DPRRIATVAFLVFALVSFLRAGFSTDIAPGNVVLPQVIQGIAMACFFIPLVAITLSGL
                     PMERVPSASGVSNFVRITCGAFGASLMTTLWERRAALHHEQLAAQVNAFNPLATQATG
                     GLQALGGDPAMSLAVLERQVTVQAFSLAANDVFWLSGVLFLVLVGTIWLTRPIHGRPA
                     PSGDAAH"
     gene            complement(13830..14597)
                     /locus_tag="PKX01_08935"
     CDS             complement(13830..14597)
                     /locus_tag="PKX01_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ76290.1"
                     /translation="MTLRVFITGASSGIGAALARRYAAQGAVLGLLARRGEALQALAA
                     ELGGTVACYVADVAEAGAVARAAADFIARHGVPDVVIANAGVSVGTLTEEAGDLAAFE
                     RVLRTNVLGLVATFQPFVAPMRARGSGRLVGIASVAGIRGLPGAAAYSASKAAAIACL
                     ESLRVELRGSGVKVVTIAPGYIRTPMTAVNTYPMPFLMDVDAAARRFVQAIAAGTSYT
                     VIPWQMGVVAKLLRLLPNPVFDALFARAGRKPRGLDL"
     gene            complement(14661..15305)
                     /locus_tag="PKX01_08940"
     CDS             complement(14661..15305)
                     /locus_tag="PKX01_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HMZ76291.1"
                     /translation="MQRRDVLKQLSAVVALAGTGLPVFAQGAGKGFELVSPPQPTDDK
                     NKVEVIEFFHYGCPHCKAFDPLLDLWIKKLPADVVFRRVPVTWGNPMLTGLARLHLAL
                     DVSGELPRLHGQVFDAVQTDKLPLNTEAGAIEWVTRKGGDGKKFADAYRSFGVQTRLA
                     RVEQLGRDYKIQGVPTLAIDGKYFTAASIAGSHEAALKVADQLIARARSEQGRK"
     gene            complement(15333..16094)
                     /locus_tag="PKX01_08945"
     CDS             complement(15333..16094)
                     /locus_tag="PKX01_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ76292.1"
                     /translation="MSRESTSRPKHAPSRSGSRGSTLVGIFVGLILGALVAAGIALYF
                     TSASPFQKQAKPDERAPAPAQPRPAPSGGEPIALPGKPGDKPVEKPRFDFYNVLPQGS
                     DALPAPSKPEETTEIVKPKPSDKSADKPAGSDDGDRAARILRGESADKTKAADAAAGD
                     KPVTPEKYYLQAGSFEDPSEADNLKARLALMGVEASVQKVDVPDKGTVHRVRVGPYLG
                     QEEMTKAKTQLASAGINAAVLRVKPKEPKPAGAAQ"
     gene            complement(16114..17847)
                     /gene="argS"
                     /locus_tag="PKX01_08950"
     CDS             complement(16114..17847)
                     /gene="argS"
                     /locus_tag="PKX01_08950"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMZ76293.1"
                     /translation="MSADPKNLLADLLRAALVSVAPEQAETPILLDRPKQAGHGDFAS
                     NLALQLAKPLKRNPRDLAALLVAELPASPLVARVEVAGAGFINFTLASDAKTRVVRQV
                     LEQGDAFGRGAKNGVKVQVEFVSANPTGPLHVGHGRGAAYGASLADVLAFAGFDVTRE
                     YYINDAGRQMDILALSTWLRYLDLFGIAIPFPPNAYQGDYVKDMAAQIREGHGDRFAK
                     VSAADVLQGAPAIDVDKETHLDALIANAKRLLGSDYAWVHGFALTEQLGDGREDLEEF
                     GVTFDKWFSERSLFDTGLVEKAVAELDARGHLYEQDGARWFRSTAFGDEKDRVVQREN
                     GLFTYFASDIAYHANKYERGFDRIIDIWGADHHGYIPRVKGALQALGLPPEKLDVALV
                     QFAVLYRDGQKAAMSTRSGEFVTLRELRNEVGNDACRFFYVLRKADQHLDFDLDLAKS
                     QSNENPVYYIQYAHARVCSVLAQWNGVVADLADADLARLDGERELALCARLAAFPEGV
                     QGAAADYAPHQIAFYLKDLAGDFHSYYNAERLLVDDEGLKLARLALAEAVRRVLASGL
                     ALLGVSAPQSM"
     gene            18011..18682
                     /locus_tag="PKX01_08955"
     CDS             18011..18682
                     /locus_tag="PKX01_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76294.1"
                     /translation="MRRRTRPFAPLLAAIAALAGGPPAHAEPPTLHWAVPLPAGQGWR
                     IVDLREGQGIRHEEYIPRGQGVDDYRDRILVQRQAAGEGMTPDTYLGYIGSGLAGHCS
                     GYTTSGLVPGQRDGLVTATRTAYCGRFDARPYGYVIAQKVIRDGEHLFIVEREWRLPP
                     FSVEPDGRTTLSPANPDEDAALKKDIRLAQRWLLEQVNPGAAPPPADPPSAPPPPVRK
                     AARKS"
     gene            18764..20296
                     /gene="ubiB"
                     /locus_tag="PKX01_08960"
     CDS             18764..20296
                     /gene="ubiB"
                     /locus_tag="PKX01_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HMZ76295.1"
                     /translation="MRLIRLVKIISVSLKYGLDQMILSNEPTGRLAWLARLARVRHFS
                     EPSGARLRMALESLGPIFVKFGQMLSTRRDLLPPAIAEELARLQDRVPPFPSGQAVAE
                     LEAAYGRPVDRVFARFDREPVASASIAQVHFAALPDGTEVAVKILRPGIAPVIAHDLA
                     LLDAFATLLEKVWPEGRRLKPREVVAEFSKYLRDELDLMREAANCSQLRRNFKDSELL
                     VVPEVYWDFCTANVMVMERMQGVPISQLDALRAHGIDLSALSRAGVEIFFTQVFRDGF
                     FHADMHPGNIFVATEGPTRGRYIALDFGIIGTLNDVDKNYLATNFLAFFQRDYRRVAL
                     AHIEAGWVPPHTRVDEFEGAIRAVCEPIFDRPLKEISFGKTLLRLFQTARRFEMEVQP
                     QLVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMNDQIGWRGLLRHVKDEAPAWA
                     AMLPQLPRLVHRALAAPHDELARAEIARLSASNRRLSVLLGIAGVLLAGGGALALWQH
                     YH"
     gene            20426..22555
                     /gene="rocD"
                     /locus_tag="PKX01_08965"
     CDS             20426..22555
                     /gene="rocD"
                     /locus_tag="PKX01_08965"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HMZ76296.1"
                     /translation="MNAPLAPPRPVRIVGVGSCLGAPIFGPAAGPRVLRDGGFEQRLR
                     RVGIPATWQTLMEPSAPKPPFPPMRERMGGVGALLNDLADEVAAIHDTGAIPLVLGGD
                     HVIGAGTWRGVARSLAPRGTLGLLWIDAHLDAHTPATTHSGNIHGMPIAALLGVGAEE
                     MTDIAGPNLDPAKLVYIGVRSWEPEEKAFLESRGVKIFYMPEVRARGLAAVLDEAVAI
                     VRDGTAGFGVSVDLDAMDCARLPATTCLVPDGLDPQDLADALHRLRSCDDLAALEIVE
                     YVPERDRHGDGARWVAEFAAAALGPTTARLRAQEARHGAHNYAPLPVVFSRGEGAWLW
                     DVEGRRYLDMMSAYSAVSFGHSHPRLVRALTEQAGRLALTSRAYSNDRLPQMLERLTA
                     SLGYDRALPVNTGLEAVETALKAARKWAYTVKGVPADQAEIIVCDGNFHGRSTTIVGF
                     SSEDQYRDGFGPFTPGFRRIPFGDADALAAALTPHTAAFLFEPIQGEGGIRIPPAGWL
                     ARCAELCRQANVLLIADEVQTGLGRTGRLLACEHEGVRPDGVILGKALGGGLLPVAAF
                     VADDAVMQVFQPGDHGSTFGGNPLSSAVADEALALLADEHLAERAATLGARFLARLQR
                     IANPLIREVRGRGLLIGLELDTRRVNARLAAEWLLARGLMTKDTHDSVLRFAPPLIVA
                     EADLDWAGDVVEEALADLARRVTGSTP"
     gene            22552..22902
                     /locus_tag="PKX01_08970"
     CDS             22552..22902
                     /locus_tag="PKX01_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HMZ76297.1"
                     /translation="MSDTLDRLVAEARKAADARAEGYRARALKLYPWICGRCAREFTV
                     ANLRELTVHHRDHNHDNNPPDGSNWELLCVYCHDNEHQRQLEATQGNIAAKQPGKAAT
                     HSPFAALGALLKKD"
BASE COUNT         3620 a         8825 c         7236 g         3423 t
ORIGIN      
        1 tctcggcgaa cagctcgcgc ccgatcagca tgcggcggat ctcgctggtg ccggcaccga
       61 tctcgtagag cttggcgtcg cgccacaggc gcccggcggc gtagtcgttg gtgtagccga
      121 caccgcccag ggtctgaatc gtctcgccag ccatccaggt ggccttctcg gcggaataga
      181 ggatcgcgcc ggccgcgtcc ttgcgcaggc tgcgggcgtg gtcgccgcgg tcgcaggcct
      241 ggccgacggc gtacacgtag gcgcgggtcg ccatccaggt ggtgtataga tcggccacct
      301 tgccctgcat gagctggaat tcaccgatgg cctggccgaa ctgcttgcgc tcgtgaagat
      361 agggcacgac caggtccatg cacgccgcca tgatgcccag cggcccgccg cacagcacgg
      421 cgcgctcgta gtcgaggccg ctcatcagca ccctggcgcc gttgccgacg ccccccagga
      481 cgttttcctc gggcacctcc acgtcgtcga agaacagcgg gaaagtgttg gacccgcgca
      541 tgcccagctt gtccaggtgc gagccgtggc tgaaaccctg catgcccttc tcgacgatga
      601 aggcggtcat gccccgcgga ccggcgtccg gatcggtctt ggcgtacacc accagagtgt
      661 cggcgtcgcc gccgttggtg atccacattt tggaaccgtt gaggacgaag cgctcgccct
      721 tgcgctcggc gcgcagcttc atgctgacca cgtcggagcc ggcgttgggt tcggacatgg
      781 ccagcgcgcc cacgtggtcg ccggcgatca ggggcggcag gtacttgcgc ttctgggcct
      841 cgctgccgtt gcggcggatc tggttgatgc acagattgga atgagcgcca taggacagcc
      901 ccaccgacgc ggaggcgcgg gacacctcct ccagggcgat gatgtgggcc agatagccca
      961 tggccgagcc gccgtagtcc tcctcgacgg tcatgccgtg caggcccagg tcgccgagct
     1021 tcttccacag atcggccggg aacaggttgt cccggtccac cgcggcggcg cggggggcga
     1081 tttccttggc tgcaaaggcc tgcacggtgt cgcgcagggc cgtgagggtc tcgccgagat
     1141 ggaaatccag gctggggatg ttcatggatg tctcctctgt ttttgtagtg tcgtgtccgc
     1201 ccgcgccctc aggacgacgg gcccggacgc gcgtgcaggt tcatgatggt ctgctgcatg
     1261 acggcgcaca agcgccaggc gccggcgcgc acggcgaaca cgtcggcgcg ggtgatgctc
     1321 aggctgcgcc cggacttcac gacctcgccc cgcgcgacca ggcactcccc ctcggccggc
     1381 gccaccagat tgagcttgta ctcaacggtc agcacctccg cgtcggccgc caccagcgtc
     1441 gccgcggcat aaccggccgc cgaatcggcc accatgccga tcacgccgcc gtggatgaag
     1501 ccgtgctgct gggtcaggtc gtcgcgagtg ggcagatgga tctcggtgca cccgggctcc
     1561 acgcgctcga gggtcgcccc gatcagcgcc atggcgcgct gtcgcgcgaa gctggcgcgg
     1621 acccgcccgg cgtaggcggg gtcgcgcggg atgaaggatt ccgggagcgg acgatgcatc
     1681 ggatgtctcc agtcggccac ctcgtgcggg cagctcgctt gaaattacgt ttacgtaaac
     1741 gtaaactaac ccataaaaaa atcaaggggt gggcatcaat tgtttttgtc catctgctgg
     1801 gccagttcgg cgcgggcttc agcggccgcg gcgggatttt cggcgaggag ttcgcggcac
     1861 tgggtctcga gcatgccgat ctccgccagc acggcaacga tgtcctcgcg ctgctgctcg
     1921 atggcggcac ggcgttcggc cagcaccacc agaaagcgcg ccagctgcgg cttggaattg
     1981 cgcacgccgt catacatgcc gatcagctca ccgatttcgg ccagcgacag gcccaggcgc
     2041 ttgccccgca ggatcaggcg caggcgcgta cgatcacgct tggaatacac ccggttgcgc
     2101 ccgacgcggc ccggcgaaag cagcccttcg tcctcataga aacggatggc gcggggggtg
     2161 atcccgaatt cacgggccag ttcggcgatg gtataggtat tttcctggct catgaggacg
     2221 ggcacgacga cgaggccgtt gcacaacggt gacaaaatgg cgcgtcggtt cagtaaatca
     2281 gatttctcga ccgcgggcaa cgcggcgctt caacccaagg aacagccatg acccaagccc
     2341 ccgacgccca actcgactac ccctgcggcg aaccgcccgc acccggcgac acccgcgaaa
     2401 tcgcccccgg cgtgcactgg atccgcatgc ccctgccctt cgccctcgac cacatcaacc
     2461 tgtggctcat cgaggacggc gacggcctga ccctcgtgga caccggctac ggcctggagg
     2521 tgacccgcga cgcctgggaa acgatcctga cccgcttcgc ccggccggtg acgcgcatcg
     2581 tcgtcaccca ctgccacccg gatcacctgg ggctcgccca gtggctcgcc gagcgcagcg
     2641 gcgcaccggt ccacctgacc ctcggtgaat tcctcgcggc ccacgccctg tggcaccagt
     2701 tgcccggtta caacgtaagc agcatggtgg cccaattccg ccgcaacggc ctcgacgacg
     2761 cccgctgcgc ggcgctggag ggtcgtggca acgcctatcg caagggcatt ccgtccctgc
     2821 ccgagtccta ctgtcggctc ctccacgacg acgtactgga catcggcggg cgcgcctggc
     2881 gggtgatttc cggctacggc catgcccccg agcacgccag cctgtactgt gccgaagcgg
     2941 gcgtcctgat ctccggcgac atgctgctgc cacgcatctc caccaacgtg agcgtcttcg
     3001 ccgccacccc ggacgatgac cccctcgccc gcttcctcga ttcgatcagc gctttcaagc
     3061 acttgccggg cgccacgctc gtgctaccat cccacggtag cccgttccgg aatatcgggc
     3121 ttcgtgtgga ccagctcgtt cagcaccacg ccgcgcgctg cgccgacctg ctcggtgcct
     3181 gcgatacgcc gcgcagcgcg ggtgagctgc tgaccacgct cttcccccgt gagctcgaca
     3241 cccaccaggt gatgttcgcg atgggggaag ccattgccca cttgaatcac cttgcacaca
     3301 aaagggaggt ctgcagggtg tcaggggaga atggtgtcgt tcgattcgtc aagatctgaa
     3361 ttaaccagga gagcacaaga aatggctgcg cccgaaaaag aagtgaccga gttgccggac
     3421 ccgaaagaaa tcgccaagac gtacgccgaa gtggcacagc gggcctccaa gctcctggcc
     3481 gaccacatgc agaagcaggt caagaagggc atcgccaccc cgtccgacga actgggcatc
     3541 gcccaggcct tcatggacat gatgggcaag ctgctcgcca acccctatcg tctggcccag
     3601 gcccagatga acctggtgtg ggactacttc tccctgtggc agcattccat gatgcgcttc
     3661 accggcatgg gcggcggcgc cccggtggcc acgcccgcca aggacgacaa gcgcttcaag
     3721 gatgaagaat ggcaggagca cttcctgttc gacttcatca agcagtccta cctcatcacc
     3781 gcccgccaca tccacgacac cgtctccacc gtggatggcc tggacgacca gtcccagaag
     3841 aaggtcaact tctacacccg ccagtacatc gacgccctgt cgccgtccaa ctttgccctg
     3901 accaacccgg aagtcttccg cgagaccgtc aagagccacg gccagaacct catcaagggc
     3961 ctcaacaacc tgctgcgcga cgtggaagac ggcggcggca acctgcgcgt gcgcatgacc
     4021 gacacctccg ccttcgaact gggccgcaac gtggccacca cgccgggcaa ggtggtgttc
     4081 cagaacgaga tgatgcagct gctgcaatac accccgagca cgcccgaggt gtccaagcgc
     4141 ccgctgctga tcgtcccgcc ctggatcaac aagttctaca tcctcgacct gcgcgagaag
     4201 aactcctaca tcaagtggtg cgtcgaccag ggtcacaccg tgttcgtgat ctcctgggtc
     4261 aacccggatg agcgcctggc cgagaagagc ttcgacagct atgtcctcga aggcaccatg
     4321 gccgccctcg acgccatcgc cgagcagacc ggcgccaagg aaatcaacgc cgccggctac
     4381 tgtctgggcg gcaccctgct cgccaccacc ctggcctact gtgccgccaa gaaggacaag
     4441 cgcctcgcct ccggcacctt cttcaccacc atgaccgact tcgccgatcc gggcgaactg
     4501 tccgtgttca tcgacgaagg ccagatcacc agcctcgaga agaagatgtt cgagcgcggt
     4561 tacctggaag gctccgagat ggccggcacc ttcaacatgc tgcgggccaa cgacctgatc
     4621 tggtccttcg tggtcaacaa ctacctgatg ggcaaggacc ccttcccctt cgacctgctg
     4681 tactggaact ccgattccac ccgcatgccg gccaagatgc acagcttcta cctgcgcaac
     4741 atgtacctgc agaacctcct caaggagccg ggcggcatcg agatcggcgg cttgcccatc
     4801 gacctgggca agatcaaggt cccgacctac ttcatctcca ccatcgaaga ccacatcgcc
     4861 ccgtggaaga gcacctactc cggcgcccgc aactttggcg gcaacgtgcg cttcgtgctg
     4921 ggcggttccg gccacatcgc cggcatcgtc aatcccccgg cggccaacaa gtacggctac
     4981 tggatcaaca cggccgccaa gatgcccgcc accgccgacg acttcttcgc cggtgccgag
     5041 cagcatcccg gctcctggtg gaccgactgg caggcctggc tgatggacca ggactccagc
     5101 aaggtgcccg cccgcgatcc cgccagcggc aagctgacgg tgctcgagga cgcgcccggc
     5161 tcctacgtca aggcccgcat cgaactgaag gccaaggcct gatcggcatc cgccgtgcag
     5221 cttgaggaag ggggcttcgg cccccttctg catttggacg cccgcccgcc attcattttc
     5281 tttgcctcat caaacctcct cgtaccttgc cgtgagcgcc cacatcctgc ccgacccgct
     5341 tcccgccggc ctgcccgtcg tcctcgacac caacaccgtg ctggccctgt ggatgttcga
     5401 ggacccgcgc ctgcctctcc tgcgtggcgc cgtcgaaagc ggccacctgc gtccattcac
     5461 ccgccaggac tgccttgacg aactgcagcg cgtcctcgcc tatgcccagt tcggccagcc
     5521 ccccgaacgt caggccgccc tcgccgccac ctacgcaacc tgctgcgcgc ggatcgaacc
     5581 ggtcgcggag cccgaggtcg tcctgcctgc ctgcctcgac cgggacgacc agaaattcct
     5641 cgaaaccgcc cgcgacgccc ccgcgcgcct cctcgtcacc cgcgacaagc tgctcctcaa
     5701 gctcgcccgc cgggcggtga tcgccgcgcg tttcgccatc ctcaccccgg aacggatcga
     5761 ggccctgctg gcccgccagg ggatggcccc gtcgccagcg ccacaatctt taacatgaca
     5821 tacaattgct gacacgccgg acccgctaga ctccctccat ccccccacca ggagcaccgc
     5881 aatgcccatc gattccagtc tggatgtcag caagctcgtc tacacctacc tcattcccct
     5941 cggctggaag atcgcaggcg ccgtcgccct gtggatcatc ggcggctggg tgatcaatgt
     6001 catcggcaac ctctccagcc gcggcatgga ccggcagaag gtcgagccca ccctctcccg
     6061 ctacgtggaa gccaccctgc gcgtcatcat gcgcatcgtg ctggtgatca tcattctgtc
     6121 ggtcttcggc atcgagacca ccagcttcgc cgccctgctg gccgcggccg gcgtggccat
     6181 cggcgccgcc tggagcggcc tgctcgccaa cttcgccgcc ggcgccttcc tgatcatcct
     6241 gcggcccttc aaggtgggtg atttcatcag cggcggcggc gtcaccggca ccgtgcgcga
     6301 aatcggcctg ttcgccacca ccgtcgatca gcccgacaac gtgcgcacca ccatcggcaa
     6361 caacaagctg ttcgccgaca acatcgtcaa ctacacctcc aaccccttcc gtcgcgtcga
     6421 gctgaccgcc cagatcgccc acagcgtcga tcccttcgag gccatcgaac tgatccgccg
     6481 gcgcatcgcc gagatcccca acgtgttggc cagccccgcg ccggacgtgg agatcctcga
     6541 gttcaacgcc gccggcacca agctcgccgt acgcccccac tgccacaacg accactactg
     6601 gcaagtgtat ttcgacacca acaaggccct cgccgaagtc ggcggcgccc atggctggcc
     6661 gatcccggcc ccgcaccagg tgctgcgtca gccgtcctga tccggtcgcc cccggcgccc
     6721 ccctccggtc cccgtcccgc cccgtgcggg gcggggattt ttcatgggcg acgcagcgcc
     6781 atcagtccgt gctcacgcgc ccgccacgcc ctcatgcagc gaacgccgca gacgcaccag
     6841 cgcccgcagg ccggcaatgg cccggctgcc gtaccaggag gcccactccc cgtcgagccg
     6901 ccgggccggc cgaccggaga gggccgtcac ctcggcctca tgcttcagct gaaagcggta
     6961 aggctccgag gacagcagca cgcgggccgc ggcctgcacg gccggctcag cccagtcaaa
     7021 cgccggatag cgtgccggcg aggccaccgc caccgcatcc cagcccaccg ccgccagggt
     7081 ggcagcaatg taggtgcccg ccgatacggt catccacgga tcgcgccaga tcaggtacag
     7141 gacccgctcc cgtggcagat cggccgccgc ggcgtacgcc tgtgccagtg catcggcgag
     7201 gccggcacac agggcctcgg cctccgccac gcgcccgaag atgccgccca ggagccgata
     7261 cagggcaaag ttgtcctgcg gcgcacaggg atgggtcacg atcacgtggg gcacgaagcc
     7321 cgccagagcc tccacggtct cgcggcggtt ctcgtccaca ttgacgatca cgtgggacgg
     7381 cgccagcgcc cgcagctttt ccaggttcac gtccttcgtc ccgcccacct tggccaccgc
     7441 acgcaagacc tcccgcgggc gcacgcagaa gccggtacgc cccaccagtt gcgcgcccag
     7501 gcccaggtcc cacaccagtt cggtgatgga cggcacgagg cagacgatgc gcgcatcccg
     7561 ggcggcgggt cgatggcaga tgcccagggc atcctgtaac ggattcacga tggcatccct
     7621 catgcttgct cactcattaa agcggcgccc ctgtcgtaaa cggaactggc agggtaacag
     7681 accgctcaga ccctggagcg accgggcatg cctcgtgcag gccgcgggtc ctgcttctcg
     7741 cgcaatcgtc ccaacacgga gcctcccatg aagatcggca ttccccgcga atccaagaac
     7801 cacgaatacc gggtcggcct gacaccgggc gccgtccgcg aagccctcgc ccatggccat
     7861 gacgtcatcg tcgaaaccgg cgccggacat ggcatcggcg ccagcgacag cgactacgaa
     7921 cgggccggcg cgcgcatcgc cctcaacgtg gacgaagtct tcggtgaggt ggagatgatc
     7981 gtcaaggtca aggaacccct cgccgccgag cgcgcccgcc tgcgcccgca ccatctgttg
     8041 ttcacctacc tgcacctggc gccggacccc ggccaggccg aagacctcct cgccagcggc
     8101 gccaccgcca tcgcctacga gaccgtcacc gcccctggcg gcggcctgcc cctcctcgcc
     8161 cccatgtccg aggtcgccgg gcgcatgagc atccaggccg gcgctgccgc cctcgagaaa
     8221 tcccacggcg gacgcggcgt gttgctgggc ggcgtgccgg gcgtggagcc gggccgggtg
     8281 gtggtcatcg gcggcggcac cgttggcaac gcggccgccc gcaccgccct cggcatgggc
     8341 gccgacgtca ccgtgctgga ccgctccctg gccgtgctgc gtcacctgga cgacgtctac
     8401 cggggccacc tgaaaaccat ccacgccacc cgcgacgcca tcgagcggca cgtcctgcag
     8461 gccgacctgg tcatcggcgc ggtgctcatc cccggcgccg cggcccccaa gctggtgagc
     8521 cgggcgatgc tcaatgccat gaagcccggt gcggtcatcg tggatgtggc catcgaccag
     8581 ggcggctgct tcgagacctc ccgccccacc acccacgccg accccaccta tgtggtggac
     8641 ggcgtcgtgc actattgcgt cgccaacatg ccaggcgccg tggcgcgcac cgccacctac
     8701 gccctcaacg ccgccaccct gccctacgtg ctggccctgg ccgacaaggg ctggaagcag
     8761 gccctcaagg acgacgtcca cctgcgcgcc ggcctcaacg tgtgcgccgg actcgtcacc
     8821 catccccagg tggccgaggc cctgggcaag aaatacgtgc cggccatcac gctgttgggt
     8881 tgaactgcgg ccgtccagcg gaaaaggctc ttgacagggc ctttttcctg cacgattatc
     8941 tgcctatgca gtcattgccc tgacaaacga tgaatccctc gcccctctac accgtcgagc
     9001 agtacgccca ggaagacagc atcggtttcc tctttcatca ggccaagcta cgcctcaccc
     9061 aggccatgga cgaacgcatc ggcgatctgg gcatcacggc cgcccaatgg gtcgtcctca
     9121 agcagatcgc cctgcgcagc ggggagaccg cctcggccct gtgcaaatgc agcggctgcg
     9181 acaccggctc gatgacgcgc atgctcgacc gcctcgaaga aaaaggtctg atccgccgcg
     9241 aacgcagcac cgccgaccgg cgcgtcgtgc agctgcacgt caccgacgcc ggccaggccc
     9301 tgctgccagc catcgtgccg cgcgtcgtgg acgtgctcaa cacggcggtc gtcgatttct
     9361 cccgcgaaga actggacatg ctcaagggcc tcatgcgccg cgtcgtcgcc aacctggacg
     9421 cccgcgctcc gcaggactga gcgcccgcct tcgcaacggg cccatgggcc catttttgtg
     9481 aacatataac tgccatgaca gatacagcac aggcaacaac atgcatcccg gaacagcccc
     9541 gcgccgtggc ccggcccctg gtcctgatca tgacgctgct cctcggcgcc tgcgtgagtc
     9601 ccgccgggct ggcaccccgc ggcacaccga ccgatccggc cacccttgcc gcgacccgca
     9661 gcctcgccga caccccggcc ggggactggc ccgcgacgac ctggtggacc gccttcggcg
     9721 acccgcagct cgaccgtctg gtcgcggccg ccctgcaggg cagcccgacc ctcgacctcg
     9781 cccgcgcccg catcgatcgg gcgcgcgccc aggccggcgt cgcggcggcc gtcagcgatg
     9841 tgcatgtggg cgccaacgtg gacggcgccc agcaacgcct gtcggaacac tacatcttcc
     9901 cgtccggcct cggcggcacg tacacgagca tcaaccgggc cgccctcgac ttcagccttg
     9961 acctggattt ctggggccgc aaccgggcag cgctggccgc cgcggtcggc gaagcccgcg
    10021 ccaccgaggc cgacgccgcc gaggcgcgcc tcgtgctggc cagcgcggtg gtgcgcagct
    10081 acctccagtt cgacctgctg caccgcctgc gcgccattgc cgacgccacc gtcacccagc
    10141 gggaacacct ccagaagctc gtgcacacgc gccagcaagc cggcctcgac gcccgcgccg
    10201 aagtccagca ggcgatcggc agcgtgtccg ccgccaaggc cgaacgcagc gccctcgaca
    10261 cccagctgga gcaggtccgt caccagctcg ccgcgctcac cggccagggc ccggacgctg
    10321 ccctggactt caccccgccc caggtgcacg ccgcccgcgt cgccctgccg gaggtgctgc
    10381 ccgccgacct cctcggccgc cgcccggacg tggtcgccca gcgctggcgc gtcgaagccg
    10441 cccgccaggg cagcgccgcc gcccgcgccg agttctaccc caacgtcaac ctgagcgcct
    10501 tcgtcggcgt acagtccctc ggcctccatc ccctcttcga aagcggcagc cgcatcgtcg
    10561 gcatcggccc ggcgatccac ctgccggtgt tcgacagcag ccgcctgcgc gccaacctcg
    10621 ccgtacgtca cggcgaagag gacatcgcca tcgcccagta caacgccacc ctggtggacg
    10681 ccctgcgcga cgtggccgac cagctcagcg tctggcgggg cgcccacgcg caggcgcgcg
    10741 accaggatgc cgccctggcc cgctacgagg aggcctggcg ccttgccacc gtgcgctacc
    10801 gcgccggcct cacgtcctac accgccgtcc tcgctgccga gacgccgcta ctcgcccaaa
    10861 ggcgcctcag cgccgaactc cacaaccgcc agctcgacgc cggcgccggc ctcgcccgtg
    10921 ccctgggcgg cgggctggcc ctcgtcacgc cccccacccc gacgacctcc accctccgct
    10981 gatcccgcca agaaggaacc ggaccatgac cgcccccacc cccgacagca cgcccaacac
    11041 cgcccgccgc cgcagcctcc tcgccatcgc caccgcctgc gggctggccg gcctggcctg
    11101 gggcggctgg tggttcaccc acggccgctt cctggagcag accgacgacg cctacgtggc
    11161 cggcaatgtc atccagatca ctccgcaagt ggccggcacc gtcatcgcca tcgacgccga
    11221 cgacacccag accgtgcgcg ccggccagac cctggtccgc ctcgacccgg ccgatgcccg
    11281 cgtcgccctg gaacaggccg aggcccagct cggccagagc gtgcgcgagg tgcaggccct
    11341 ctacgccggc ggcggggccc tcgaagccac cgtccgtcag cgcgaggccg atctcgcccg
    11401 ggcccgcgac gatctgcagc gccgccagca ggtggccggc agcggcgccg tggccgagga
    11461 agagatcgag catgcccgcg ccgccctgcg cgccgccgaa acagccctgc agaccgcccg
    11521 cgagcagctc gtcaccaacc aggtgcagac ccgcggcacc cagatcgcca cccaccccaa
    11581 cgtgcagcgc gccgccgccc gcgtggagga gagctacctc gcctacgccc gcagcgccgt
    11641 gccggcgccg gtgggtggac tggtggcacg gcgctcggtg caggtcggtc agcgcgtggc
    11701 cgccggcgcg ccgctgatgg ccgtcgtacc cctcgatcag gtgtgggtgg acgccaactt
    11761 caaggaaagc cagctccacg gcatgcacgc cggccagccg gtgaccctcg ccgccgacgt
    11821 ctatggcagc cgcgtgacct accacggcca catcgccggc ctcgccgccg gcaccggcgc
    11881 cgctttcgcc ctgctgccgg cccagaacgc cagcggcaat tggatcaagg tggtgcagcg
    11941 ggtccccgtg cgcatcgccc tcgacccccg ggagctggcc gcccacccgc tgcgcatcgg
    12001 cctgtccatg caggtcgagg tggatctgcg cgacagcacg gacagcgacg cccccctcgc
    12061 ccaggccgtg cccgccagcc cggtggccgc taccgatgtc tatgccgagc tcggcaaggc
    12121 tgcgcgggcg cgcatccagg ccatcatcgg cgccaacagc ggcggtcccg cggcgccccc
    12181 gctcgccgag ccggtaacgc ccggcacgac gcctgcggcc gctgcccagg ccaccgcccc
    12241 gcggcgcacc caggcctgac atcatggccg gccccatcgc cctgccgccc ctcgaaccgg
    12301 gggcgcgccg cctcgccacc gtcgccctgt ccctggcgac cttcatgaac gtgctggaca
    12361 cgaccatcgc caacgtgtcc ctgcccaaca tcgcggggga cctgggggtg agcccgagcc
    12421 agagcacctg ggtcatcacc tccttcggcg tggccaatgc catcgccgtg cccctcaccg
    12481 gctggctcag ccagcgcttc gggcaggtgc gcctgttcgt cacctcggtg gtgctgttcg
    12541 tcatcgcctc gtggctgtgc ggtctggccc acagcctgga gatgctcatc gccgcccgtg
    12601 tcctgcaggg ggccgtggcg ggccccatga tccccctctc ccaggccctg ctgctgcaga
    12661 gttacccgcg ggaaaagtcc ggcatggccc tcgccatgtg gtccatgacg accctggtgg
    12721 ccccggtgat gggccccatc ctgggcggct ggctgtcgga caacctgtcc tggccgtgga
    12781 tcttctacat caacctgccg gttggcctcg gcgccgcgtg gatgagctgg cagctcctgc
    12841 gcgagcggga gacgcccacc cgcaagctgc ccatcgaccg ggtcgggctc gtcctgctcg
    12901 tggtgtgggt cggcgccctg cagatcatgc tcgacaaggg caaggagctg gactggttcg
    12961 aatccggcga gatcgtcgcc ctcggcgccg tcgccctggt gggcttcgcc ctgttcgtgg
    13021 cctgggagct gaccgaggcc cacccggtgg tggacctcac cctcttcgca cgacccaact
    13081 tcctgttcgg cgtgatcgcc ctggccgccg gctacggcgt gttcttcggc aacgtggtgg
    13141 tgatgcccct gtggctgcag cagttcatgg gctacaccgc cacctgggcg ggtctcgtta
    13201 ccgcgcccat tggtcttctc gcgctgctcc tgtcaccctt catcgggcgc atcatgccgc
    13261 gcaccgatcc gcgccgcatc gccaccgtgg ccttcctggt gttcgccctg gtgtccttcc
    13321 tgcgcgcggg attttcaacc gacatcgcgc cgggcaacgt ggtgctgccc caggtgatcc
    13381 agggcatcgc catggcctgc ttcttcattc cgctggtggc catcaccctg tcgggcctgc
    13441 cgatggagcg ggtgccctcc gcctcgggcg tgtcgaactt cgtgcgcatc acctgcgggg
    13501 ccttcggcgc ctcgctcatg accaccctgt gggagcgccg cgccgcgctg catcacgaac
    13561 agctggcggc ccaggtgaat gccttcaacc ccctcgccac ccaggccacc ggcggcctcc
    13621 aggcgctggg cggcgacccg gcaatgagcc tcgcggtgct ggagcggcag gtcaccgtgc
    13681 aggccttctc cctcgccgcg aacgacgtgt tctggctgtc tggcgtgctg ttcctggtcc
    13741 tggtcggaac gatctggctg acccgaccga tccacggccg tcccgccccg tccggagacg
    13801 ccgcccactg acccgcggcg ccgcgcaggc tacaggtcga gcccgcgcgg cttgcgcccg
    13861 gcacgggcga acagggcgtc aaagacggga ttgggcagca ggcgcagtag cttggccaca
    13921 acgcccatct gccacgggat cacggtgtag gaggtgcccg ccgcgatggc ctgcacgaag
    13981 cggcgggccg ccgcgtccac gtccatcagg aagggcatcg gataggtgtt caccgcggtc
    14041 atcggcgtgc ggatgtagcc cggcgcaatc gtcaccacct tcacgccgct gccgcgcagt
    14101 tccacccgca ggctctccag gcaggcgatg gccgccgcct tggacgcgct gtaggccgcg
    14161 gcgccgggca ggccgcggat gcccgccacg gacgcgatgc ccaccaggcg accgctgccc
    14221 cgcgcccgca tgggcgccac gaagggctgg aaggtcgcca ccaggcccag cacattggtc
    14281 cgcagcaccc gctcgaaggc cgccagatcg ccggcctcct cggtgagagt cccgaccgac
    14341 acgccggcat tggcgatcac cacgtccggc acgccgtgcc gggcgatgaa atccgccgcc
    14401 gcgcgggcca cggcgcccgc ctccgccaca tccgcgacat aacaggccac cgtgccgccc
    14461 aactcagcag ccagggcctg caaggcctcg ccgcgccgcg ccagcaggcc cagcacggcg
    14521 ccctgggcgg catagcgacg agccagggcc gcgccgatgc cgctcgacgc accggtgatg
    14581 aacacccgca gggtcatggg gaaacaggaa acgtggggaa tgtgcgccca cccgacccaa
    14641 ggccgggcag gcaggagaac ttacttgcgg ccctgctcgc tgcgggcgcg ggcgatgagc
    14701 tggtcggcca ccttgagggc cgcctcatgg ctgccggcga tggacgccgc ggtgaagtac
    14761 ttgccgtcga tggccagggt cggcacgccc tggatcttgt agtcacggcc gagctgttca
    14821 acccgcgcca ggcgggtctg cacgccgaag gagcgatagg cgtccgcgaa cttcttgccg
    14881 tcgccgccct tcctggtcac ccactcgatg gcgccggcct cggtgttgag gggcagcttg
    14941 tcggtctgca ccgcgtcgaa gacctggccg tgcaggcgcg gcagttcgcc ggatacgtcg
    15001 agggccagat gcaggcgggc cagcccggtc agcatcgggt tgccccaggt cacgggcacg
    15061 cggcggaaca ccacgtccgc cggcagcttc ttgatccaca ggtccagcag cggatcgaag
    15121 gccttgcagt gggggcagcc gtagtggaag aactcgatga cttccacctt gttcttgtcg
    15181 tccgtcggct ggggcggcga caccagctcg aaacccttgc ccgcgccctg ggcgaacacg
    15241 ggcaggccgg tgccggcgag ggcgacgacg gcggaaagct gcttgagcac atcacgacgc
    15301 tgcattgcgg aatctcctgg ttcgcaaaga ccttattgag cagccccggc gggctttggt
    15361 tccttgggct tgacccgcag cactgcggca ttgatgccgg cggaggccag ctgggtcttg
    15421 gccttggtca tttcttcctg gcccagatag gggcccacgc gcacccgatg cacggtgccc
    15481 ttatccggca catccacctt ctgcacgctg gcttccacgc ccatcagggc gagccgcgcc
    15541 ttgaggttgt cggcctcgga cgggtcttcg aaagagccgg cctggaggta gtacttctcc
    15601 ggcgtgaccg gcttgtcgcc ggcggcggca tcggccgcct tggtcttgtc cgccgactcg
    15661 ccccgcagga tgcgcgcggc gcggtcgccg tcgtcgctgc cggcgggctt gtccgccgac
    15721 ttgtcggatg gcttgggctt gacgatctcg gtggtctctt cgggcttgga cggcgccggc
    15781 agggcatcgg atccctgggg cagcacgttg tagaagtcga agcggggctt ctccaccggc
    15841 ttgtcgcccg gcttgccggg cagggcgatg ggctcgccgc cggaaggcgc cgggcggggc
    15901 tgcgctggcg cgggggcgcg ctcgtccggc ttggcctgct tctggaaggg cgaggccgag
    15961 gtgaagtaca gggcaatccc cgccgccacc agtgcgccca ggatcaggcc gacgaagatg
    16021 ccgaccaggg tgctgccccg gctgccggag cgggacggcg cgtgcttcgg gcgggacgtg
    16081 gattcgcggc tcatggacag gatttccgga cactcacatg gattggggcg cgctcacgcc
    16141 gagcagggcc agaccgctgg ccagcacgcg gcgcacggcc tccgccaggg cgaggcgggc
    16201 cagcttgagg ccctcgtcgt ccaccagcag gcgctcggcg ttgtaatagc tgtggaaatc
    16261 gccggccagg tccttgaggt agaaggcgat ctggtggggc gcgtagtccg ccgcggcacc
    16321 ctgcaccccc tccggaaagg ccgccagacg ggcacacagg gccagctccc gctcgccatc
    16381 gaggcgcgcc aggtcggcgt cggccagatc ggccaccacg ccgttccact gggccagcac
    16441 cgagcacacg cgggcgtggg cgtactggat gtaatacacc gggttctcgt tgctctggct
    16501 cttggccaga tccaggtcga aatccagatg ctggtcggcc ttgcgcagca cgtagaagaa
    16561 gcggcaggcg tcgttgccca cttcgttgcg cagctcacgc agggtgacga actcgccgga
    16621 gcgggtggac atcgccgcct tctggccgtc gcggtaaagc accgcgaact gcaccagcgc
    16681 cacatcgagc ttctcgggcg gcaggcccag cgcctgcagg gcgcccttca cgcgggggat
    16741 gtagccgtgg tggtcagcgc cccagatgtc gatgatgcgg tcgaagccgc gctcgtactt
    16801 gttggcgtgg taggcgatgt cggaggcgaa gtaggtgaac aggccgttct cccgctgcac
    16861 gacgcggtcc ttctcgtcgc cgaaggcggt ggagcggaac cacctggcgc cgtcctgttc
    16921 gtagaggtgg ccacgggcgt ccagttcggc cacagccttc tcgaccagac ccgtgtcaaa
    16981 cagggagcgc tcggagaacc acttgtcgaa ggtgacgccg aactcctcca gatcctcgcg
    17041 gccgtcaccg agctgctcgg tgagggcaaa accatgcacc cacgcgtagt cgctgcccag
    17101 caggcgcttg gcattggcga tcagggcgtc caggtgggtt tccttgtcca cgtcgatggc
    17161 cggcgcgccc tgcagcacgt cggcggcgga gaccttcgcg aagcggtcgc cgtggccttc
    17221 cctgatctgc gccgccatgt ccttgacgta gtcgccctgg taggcgttgg gcgggaaggg
    17281 aatggcgata ccgaacagat ccaggtaacg cagccaggtg gacagggcca ggatgtccat
    17341 ctgccggccg gcgtcgttga tgtagtactc gcgggtcacg tcgaagcccg caaaggccag
    17401 cacgtcggcg agggacgcgc cgtaggccgc gccgcggccg tggcccacgt ggagcggccc
    17461 ggtggggttg gcggagacga actccacctg taccttgacg ccgttcttgg cgccacgccc
    17521 gaaggcgtcg ccctgctcca gcacctgacg caccacgcgg gtcttggcgt cggaggcgag
    17581 ggtgaagttg atgaagccgg cgcccgccac ctccaccctg gccaccagcg gcgaggcggg
    17641 cagctcggcc accagcaggg cggccaggtc acgcggattg cgcttgaggg gcttggccag
    17701 ctgcagcgcc aggttgcttg cgaaatcccc gtgcccggcc tgcttgggac ggtcgaggag
    17761 gatgggggtt tcggcctgtt cgggggccac gctgacgagg gcggcgcgga gcaggtcggc
    17821 aagcaggttc ttcggatcgg cgctcatgga acacacagca actaaaagcg tgaaattata
    17881 gcgaagcgcc gcacttcccg cctgttgctg gtgtgaccga tgtccggcgc attcccgact
    17941 ggcacacgga gaattgttgt cttatgtttc agggattgca tgcaggtccc gctccgacga
    18001 cgctgatacc gtgagacgcc gcacccgccc cttcgccccg ctgctcgctg ccatcgccgc
    18061 cctcgccggt ggcccgcccg cgcacgccga gccgcccacc ctgcactggg ccgtgccgct
    18121 gccggccggc cagggctggc gcatcgtcga tctgcgcgaa ggccagggca tccgccacga
    18181 ggaatacatc ccccgcggcc agggcgtgga cgactaccgc gaccgcatcc tggtgcaacg
    18241 ccaggccgcc ggcgaaggca tgacgcccga cacctatctg ggctacatcg gctccgggct
    18301 ggccgggcac tgctcgggct acaccaccag cggcctggtg ccgggccagc gcgacggcct
    18361 cgtcaccgcc acccgcaccg cctactgcgg ccgcttcgac gcccgcccct acggctacgt
    18421 catcgcccag aaggtcatcc gcgacggcga gcacctcttc atcgtcgagc gggaatggcg
    18481 cctgccgccc ttcagcgtcg agccggacgg ccgcaccacc ctcagccccg ccaacccgga
    18541 cgaggacgct gccctcaaga aggacatccg cctcgcccag cgctggctgc tggagcaggt
    18601 caatcccggc gctgcgccgc cgccggccga tcccccgtcc gccccgccgc cccccgtccg
    18661 caaggccgcc cgcaaatcct gaggcccgcc gggcggtcgg accgccctat gcgcagcagg
    18721 gctttacggg cacaatcgcc gtttttccga ccgccctccc gccgtgcgcc tcatccgact
    18781 cgtcaagatc atctccgtca gcctgaaata cgggctcgac cagatgatcc tctccaacga
    18841 gccgaccggc cgcctggcct ggctggcgcg gctggcgcgg gtccgccact tcagcgaacc
    18901 cagcggcgcg cgcctgcgca tggcgctgga atccctcggg ccgatcttcg tcaagttcgg
    18961 gcagatgctg tccacccggc gcgacctgct gccgccggcc atcgccgaag aactcgcgcg
    19021 cctccaggac cgggtgccgc ccttccccag cgggcaggcc gtggccgagc tggaggccgc
    19081 ctacggccgc ccggtggacc gggtcttcgc ccgcttcgac cgggaacccg tcgcctccgc
    19141 ctccatcgcc caggtgcact ttgccgccct gcccgacggc accgaggtgg cggtcaagat
    19201 cctgcgcccc ggcatcgccc cggtcatcgc ccacgacctg gccctcctcg acgccttcgc
    19261 caccctgctg gagaaggtct ggcccgaagg ccgccgcctc aagccgcggg aggtggtggc
    19321 cgagttctcc aaatacctgc gcgacgagct cgacctgatg cgcgaggccg ccaactgttc
    19381 ccagctgcgg cgcaacttca aggattccga gctcctcgtc gtgcccgagg tgtactggga
    19441 tttctgcacc gccaacgtga tggtgatgga gcgcatgcag ggcgtgccca tctcccagct
    19501 cgacgccctg cgcgcccacg gcatcgacct cagcgccctg tcccgcgccg gcgtggagat
    19561 cttcttcacc caggtcttcc gcgacggctt cttccacgcc gacatgcacc ccggcaacat
    19621 cttcgtcgcc accgaagggc ccacccgcgg gcgttacatc gccctggatt tcggcatcat
    19681 cggcaccctc aacgacgtgg acaagaacta cctggccacc aacttcctgg ccttcttcca
    19741 gcgcgactac cggcgcgtcg ccctggccca catcgaggcc ggctgggtgc caccccacac
    19801 ccgcgtggac gaattcgaag gcgccatccg cgccgtctgc gaacccatct tcgaccggcc
    19861 gctcaaggaa atctccttcg gcaagaccct gctgcgcctg ttccagaccg cccgccgctt
    19921 cgagatggaa gtgcagcccc agctcgtcct gctgcagaag accctgctca acatcgaagg
    19981 cctgggccgt cagctcgacc cggacctgga cctgtggaag accgccaagc ccttcctcga
    20041 gcgctggatg aacgaccaga tcggctggcg cggcctgctc cgccacgtca aggacgaagc
    20101 ccccgcctgg gccgccatgc tgccccagct gccgcgcctg gtgcatcgcg ccctggcggc
    20161 gccccacgac gagctcgccc gcgccgagat cgcccgcctc agcgccagca accgccgcct
    20221 gagcgtgctc ctgggcatcg ccggcgtgct gctggccggc ggcggcgccc tcgcgctctg
    20281 gcagcattac cactgaggcc ggggcaatcc ggccccgtcg actgcccatt ccgtcacgcc
    20341 gccgcccgcc ttccccagaa caaagccgcc ctgccgacgc tccaagcctg aaagggttgt
    20401 cggcctgccc ccagagagac gtgccatgaa cgcccccctc gctcctccgc gcccggtccg
    20461 catcgtcggc gtcgggtcct gcctcggcgc ccccatcttc ggtcccgccg ccggcccccg
    20521 cgtgctgcgc gacggcggct tcgaacagcg cctgcgccgc gtcggcatcc ccgccacctg
    20581 gcagaccctg atggaaccct ccgcgcccaa gccgcccttt ccgcccatgc gggaacgcat
    20641 gggcggcgtc ggcgccctcc tcaacgacct cgccgacgaa gtggccgcca tccacgacac
    20701 cggcgcgatt ccgctggtcc tcggcggcga ccacgtcatc ggcgccggca cctggcgcgg
    20761 cgtggcgcgc agcctcgccc cccgcggcac cctcggcctg ctgtggatcg acgcccacct
    20821 cgacgcccac acccccgcca ccacccacag cggcaacatc cacggcatgc ccatcgccgc
    20881 cctgctgggc gtgggcgccg aagagatgac cgacatcgcc ggcccgaacc tggacccggc
    20941 caagctggtg tacatcggcg tgcgctcctg ggagcccgaa gagaaagcct tcctcgaaag
    21001 ccgcggcgtc aaaatcttct acatgcccga agtgcgcgcc cgcggcctcg ccgcggtgct
    21061 cgacgaagcc gtcgccatcg tccgtgacgg caccgccggc ttcggcgtgt cggtggatct
    21121 ggacgccatg gactgcgccc gcctgcccgc caccacctgc ctggtgcccg acggcctcga
    21181 cccgcaggat ctggccgacg ccctgcaccg cctgcgcagc tgcgacgatc tggccgccct
    21241 ggagatcgtg gaatacgtgc ccgagcgcga ccgccacggc gatggcgccc gctgggtggc
    21301 cgagttcgcc gccgccgccc tgggccccac caccgcccgc ctgcgggccc aggaagcccg
    21361 ccacggcgcg cataactacg cgcccctgcc ggtggtcttc agccgcggtg aaggcgcctg
    21421 gctgtgggac gtggaagggc gccgctacct ggacatgatg agcgcctact cggcggtgag
    21481 cttcggccac agccacccgc gcctggtgcg cgccctcacc gagcaggccg gccgcctggc
    21541 gctgacctcc cgcgcctact ccaacgaccg cctgccgcag atgctggagc gcctcaccgc
    21601 cagcctcggc tatgaccgcg ccctgcccgt caacaccggc ctggaagccg tcgagaccgc
    21661 cctcaaggcc gcccgcaagt gggcctacac cgtcaagggc gtgcccgccg accaggccga
    21721 gatcatcgtc tgcgacggca acttccacgg ccgctccacc accatcgtcg gcttctcgtc
    21781 cgaagaccag taccgggacg gcttcggccc cttcacgccg ggcttccggc gcatcccctt
    21841 cggcgacgcc gacgccctcg ccgccgccct cacgccccac accgccgcct tcctcttcga
    21901 acccatccag ggcgaaggcg gcatccgcat cccgccggcc ggctggctcg cccgctgcgc
    21961 cgagctgtgc cgtcaggcaa acgtcctgct catcgccgac gaggtgcaga ccggcctcgg
    22021 ccgcaccggc cgcctcctcg cctgcgagca cgaaggcgtg cgcccggacg gcgtgatcct
    22081 cggcaaggcc ctcggcggcg gtctcctgcc ggtggccgcc ttcgtggccg acgacgcggt
    22141 gatgcaggtc ttccagcccg gcgaccacgg ctccaccttc ggcggcaacc ccctctccag
    22201 cgccgtcgcc gacgaagccc tggccctgct cgcggacgaa cacctcgccg aacgggccgc
    22261 caccctcggc gcccgcttcc tggcccgcct gcagcgcatc gccaaccccc tcatccgcga
    22321 ggtgcgcggc cgcggcctgc tcatcgggct ggagctggac acccgccggg tcaacgcccg
    22381 cctcgccgcc gaatggctgc tcgcccgcgg cctgatgacc aaggacaccc acgacagcgt
    22441 gctgcgcttc gccccgccgc tgatcgtcgc cgaagccgat ctggactggg ccggcgacgt
    22501 ggtggaagaa gccctcgccg atctggcccg acgcgtcacc gggagcaccc catgagcgac
    22561 acactcgacc gcctcgtcgc cgaggcccgc aaggccgccg acgcccgcgc cgaaggctac
    22621 cgcgcccgcg ccctcaagct ctacccgtgg atctgcggcc gctgcgcgcg ggaattcacc
    22681 gtcgccaacc tgcgcgaact caccgtccac caccgcgacc acaaccacga caacaacccg
    22741 ccggacggca gcaactggga gctgctgtgc gtgtattgcc acgacaacga acaccagcgc
    22801 cagctcgaag ccacccaggg caacatcgcc gccaaacagc ccggcaaggc cgccacccat
    22861 tcccccttcg ccgccctcgg cgcgctgctg aagaaagact gaggccgccg cgcggcgcgc
    22921 gcccgccacc gcggccctga agccgccacc tcgggccgtg cacaccgccg acgggccgat
    22981 ctacccttcc gccgcagccc ggcccccgca gacgattccc gcgccgccca atccggcgta
    23041 ccatcggcgc ccccgcgcac ctccggcctg cccatgacat ccgctcccgc ccgcgcctgc
    23101 ggca
//
LOCUS       DAOQPA010000052        22462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1099_length_22462_cov_19.565314, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000052 DAOQPA010000000
VERSION     DAOQPA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 22462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22462
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_22462_cov_19.565314"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKX01_08975"
     CDS             complement(<1..878)
                     /locus_tag="PKX01_08975"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HMZ76298.1"
                     /translation="MLEAYRQHVAERAALGIPPLPLSKKQAQDLVALLQNPPAGEEAF
                     LVELLTYRVPAGVDDAAKVKAEFLAKVAKGELACGLVSRAKATELLGTMLGGYNVKPL
                     IDLLADAEVGAVAAEGLKKTLLVFDYFHDVAELGKAGNANAKAVMQSWADAEWFTSRP
                     EVPAVQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADEPGSRG
                     PIKQLEALAAKGNLIAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGS
                     KIAPIFFNTMEDAGALP"
     gene            complement(1194..1583)
                     /locus_tag="PKX01_08980"
     CDS             complement(1194..1583)
                     /locus_tag="PKX01_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76299.1"
                     /translation="MSSPLTFQDKHVLIVDDNPINRTLAAAFTKRLGITSDQAADGAT
                     ALALISANRYDAVLLDISMPGMSGEEVLARIRSDAVFGSLPVIAYTAHALPDEKQGLL
                     AAGFDDLLIKPVTMGAMEAVLASVLTG"
     gene            complement(1580..4150)
                     /locus_tag="PKX01_08985"
     CDS             complement(1580..4150)
                     /locus_tag="PKX01_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76300.1"
                     /translation="MQTNTGRFGIRGKLISIFIVIKVVPLVLLALLAWTMAEKLGDAV
                     GLQLESVSSSMLRTLRSVGEEATQDALHALDDRARESMERLTTDTARAVAAFLYDRDA
                     DIREAAELEPGDASYRRFLAHRQRPVFSHGPWKLADDGKRWEPAAPESWDESLAADAG
                     QALSDNAKAFSARPPEFLGHREMRPLFVEMSFIDLSGRERVKVTQGSLMTPGLRQVSD
                     ARETFIRAEHYWPELQKLKPGEIHVSDVVGAYVKSHVIGPYLPASAEKAGEPFKPEAS
                     AYAGTENPVGRHFRGIVRWAMPVVRQGQITGYVTLALDHDHIRQFTDRISPTDARYTP
                     INDAIAGNYAFMWDHRGRAISHPRDYFIPGYDPATGEPVTPWMDQSLYEAWQSSGKSS
                     QAFLEGVKPYDDQSLKKKPAGALVKAGTVALDCRYLNFSPQCSGWKLLTEKGGSGSFV
                     IFFSGLWKLTTAAAIPYYTGPYAASPQGFGIVTIGANVDDFHRAATESAKRIGLTIDA
                     KDKEFQTLRAGISQAINDNLTQTAGALAISTLVMVAVVIAVAIWMAQFITRRITRMIE
                     GLDGFQAGDLSRRLEVKSTDEMGHLAESFNSMADRVQESFKRSEDARQSAEAANRMKS
                     EFLANISHELRTPLNGILGFAELLKLDATDAAHQEYAATIESSGQHLLDLVNDLLDLA
                     KVEAGRMELNLSDVPLATFAQEVCAAHRGHAQAKGLQLECTIAAGTPERLHTDPLRLR
                     QILNNLLNNAIKFTDNGRIDLNIHADGERIALAVSDTGRGIPKDAQDVIFDKFRQVDA
                     FVTREQAGTGLGLALARQFVHLLGGELSLRSEPGVGSTFTLLLPRDAATTARPTASPR
                     "
     gene            4327..4707
                     /locus_tag="PKX01_08990"
     CDS             4327..4707
                     /locus_tag="PKX01_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HMZ76301.1"
                     /translation="MSDPCPCGSGRAYPACCGPYLDQSLPAPTAEALMRARYTAYTLK
                     REDYLLATWDPAHRPAQLDLASDTATRWLGLQVKRHETTGSGQAIVEFVARYKVAGRA
                     YRLHETSRFHVLGGAWVYVDGDVH"
     gene            complement(4720..4935)
                     /locus_tag="PKX01_08995"
     CDS             complement(4720..4935)
                     /locus_tag="PKX01_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMZ76302.1"
                     /translation="MATRFPVQGDFIQLDQLLKATGLCDSGGVAKQRVAEGQVKVDGV
                     IETRKRAKLRPGQRVEFAGEVIELVAT"
     gene            complement(5023..5511)
                     /gene="lysM"
                     /locus_tag="PKX01_09000"
     CDS             complement(5023..5511)
                     /gene="lysM"
                     /locus_tag="PKX01_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HMZ76303.1"
                     /translation="MGLLSFVREAGEKLFGRKDVEAAAADSGAAERLADLNAKAAAAI
                     LAYIGTQGLDASALSVSFDGATSTVTASGSVSDQATKEKILLCCGNVDGVEQVIDQLA
                     VTHPEPEAQFHTVVRGDTLSAIAKHYYGNANAYMKIFEANRPMLGHPDKIYPGQMLRI
                     PT"
     gene            complement(5606..5938)
                     /locus_tag="PKX01_09005"
     CDS             complement(5606..5938)
                     /locus_tag="PKX01_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ76304.1"
                     /translation="MSHPCLSCGACCASFRVDFHVSDLASHPGGGVPDALTVPVTATL
                     VRMRGTDDGPPRCVALSGEVGREVSCSIYEQRPGPCRDFAPYAALGIGDEGCARARRR
                     HGLPPLGA"
     gene            complement(5945..6643)
                     /locus_tag="PKX01_09010"
     CDS             complement(5945..6643)
                     /locus_tag="PKX01_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HMZ76305.1"
                     /translation="MNPIPVSMPLTEEELEQLEELLASDDVPADCMNMEMLDGYLAAV
                     VAAPGPIPVEVWLPAVWTADGDEAGFGSGAVLQKAIELVLRYHNDLLLAIGDDEADDA
                     WQPFCYAQGGDDDPAIGEEWTAGFEQGFELWPTEWTDTVEDTSIAEALLETLLGDDEE
                     DAVADSAEQLDILEAKGIAVHRLVQTWRALGLAAPEPVVVAAAAGAAPAGPGRNEPCP
                     CGSGKKYKKCCGAT"
     gene            complement(6640..7326)
                     /locus_tag="PKX01_09015"
     CDS             complement(6640..7326)
                     /locus_tag="PKX01_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ76306.1"
                     /translation="MYANSRIPESAQSGLHEALESRVNKHAATDFRKPYAPYNVAAFE
                     ASLAGWDQRAPLLLDAGCGVGHSTIALARAFPDHYVIGVDQSEDRLSRKKPYPDALMP
                     TNLVFCRADLVDYWRLLADAGIRLDRHYILYPNPWPKIGHLTRRWHAHPVFPCVPRLG
                     GRLECRSNWPIYIAEFARAVELLTGVPAPWQPLEAPSPLTPFERKYRDSGQTLYQCVV
                     ELDQLEEQSS"
     gene            complement(7336..8319)
                     /locus_tag="PKX01_09020"
     CDS             complement(7336..8319)
                     /locus_tag="PKX01_09020"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HMZ76307.1"
                     /translation="MPRLYLAPMEGLADDVLREVLTRIPGYDHAVTEFLRVSATVLPG
                     RSFTRIAPELKVGSRTSSGTPVHLQLLGSDPQRMAASAARLVRLHPAGIDLNFGCPAP
                     TVNRHRGGAALLDEPELLHAIACAVRASVPKGIPFTAKMRLGIADTGRTLDCARALAD
                     GGIDALVVHARTKVDGYRPPAHWEWVGRIADTVGVPVAANGEVWSEADWQRCRAVSGV
                     SDVMLGRGAVADPFLARRIRALQADGTKTPDRDAEWAELSPLLGEFWWRVRAKLEARH
                     APGRLKQWLNLLRRNFPQAESLYGEIRALKDCTAVEGHLRRHGVPVLSAAA"
     gene            8492..9829
                     /locus_tag="PKX01_09025"
     CDS             8492..9829
                     /locus_tag="PKX01_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HMZ76308.1"
                     /translation="MRRIDFTNRREWLTLALPALLLVVAAFAVAWKFVQPAPPRQIVM
                     STGPAGGAYQYFAERYRERLARDGIELALRPSTGAVENLNRLKTVDEVDMGFVQGGVA
                     DEPESDDLATLGSMYLEPVWVFFRGKADLDRIDQLKGRRIAVGAPGSGTLLFALQLLS
                     ANGFATDDPHFIHLGGLEAVEAIRSGQVDAVFAVGAPQAPAIQALLAEPGLSLLNFAQ
                     ADGYVRHFPHLSRITLPRGAIDIAQDRPPRDIHLLAATANLVVMADLHPAIVTRVLQH
                     AAQIHGQPGMFMTPFAFPANQDRSLPLHPSARRFYESGPPFLQRYLPFWLAVLIDRLI
                     VMLVPIVAVVIPLFKVLPALYNWRMRSRVYRWYGELKFLENEIGQQSTHTPEVLDDFI
                     RRLDGIEDRASRRKLPLAFSNELYTLREHINLVRQRLERLQARAQVPAAPTVS"
     gene            9885..10496
                     /locus_tag="PKX01_09030"
     CDS             9885..10496
                     /locus_tag="PKX01_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ76309.1"
                     /translation="MSPHLDLTEPYAPLVRRIEAEATVTTDGNLRIDHFLNHRVEPAY
                     IAELGHELARRLSYFQPNVILTAEASGIAPALIVAQVLSIPMVYAKKYSCDVEGPAIS
                     RIVASPGRGAEVRLALSSRYVRSGHRVVVVDDFLSNGRTAAALVEMAREAGAEVLGAG
                     FIVEKRFNRGREHIAAMNVPIATLAQIEFLHDGRACLTQPPAA"
     gene            10505..11800
                     /locus_tag="PKX01_09035"
     CDS             10505..11800
                     /locus_tag="PKX01_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HMZ76310.1"
                     /translation="MSPRQILRSLFDAALAAAQPERVVPLHLPDGGALPRGRVIVIGA
                     GKASAAMARAVEDHWPGDPARLSGLVITRYGHGVPCRHIEIVEAAHPVPDERGYAAAR
                     RMLEHTVAELGPDDLVLCLISGGGSALLAVPAAGITLADKQAIGRALLASGATIGEIN
                     CVRKHLSAIKGGRLALACQPARVLTLLISDVPGDDPSIIASGPTVGDPTTCAEALTVL
                     DKYAIPLPAHVRAHLESARGESPKPGHPAFARCETRLIATPQQALEAAARRARELGLS
                     AHILSDAMEGESRHVARVHAALARQVADRGQPFVAPCVLLSGGETTVTVSGKGQGGRN
                     VEFLVALAQALDGHPRVHALAADTDGVDGSLDIAGACIDPTTPARTLALGRRLADVLA
                     DNDGHGLFQALGDSVVTGPTQTNVNDFRAILIDAPDAAL"
     gene            complement(11829..12731)
                     /locus_tag="PKX01_09040"
     CDS             complement(11829..12731)
                     /locus_tag="PKX01_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76311.1"
                     /translation="MSSFSSRWRAEMCRGSSRWRAGVLMGALLVSGARAECPADVEAA
                     AVAAAIVNRTPVHAVPGLTRMEDGLCGRDKVVRFLAQAWGAPVGHKAGLTNPAVQQRF
                     GYGAPLRGRLFRAGLLPDGAQVPAAFGIRPVFEADLLVEVADPHALARAGSPDAALQA
                     LSAVIPFVELPDLMVGDPGALSGPMLALVNVGARLGVQGTSVPARSGGLRAEALASMT
                     VSVRDGTGAVLDSAQGSAILGHPLNAAIWLARDLAAAGTPLQRGDLLSLGSFSRLLAP
                     KSGLDIVVRYDGLPGNPEVRVHFR"
     gene            complement(12700..13515)
                     /locus_tag="PKX01_09045"
     CDS             complement(12700..13515)
                     /locus_tag="PKX01_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ76312.1"
                     /translation="MAHGLLKFFRSVLLGLAVSTAAVQAGESIVVASTTSTEQSGLFN
                     FILPIFEKDSGIAVRVVALGTGQALDVGRRGDADVVLVHDRPAEDKFVADGYGVDRRD
                     VMYNDFVVIGPAADPAGVKRARDASDAFARIGSQGATWISRGDRSGTHAAELRFWKAA
                     GVDPRGQGWYKEAGAGMGPTLNMAAGLGAYTLSDRGTWASFRNRQDLGILFAGDPQLY
                     NPYGVILVNPARHPHIKRAAALRFMDWLTSADGRRAIAAYKIGNEQLFFPLAR"
     gene            complement(13497..14210)
                     /locus_tag="PKX01_09050"
     CDS             complement(13497..14210)
                     /locus_tag="PKX01_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ76313.1"
                     /translation="MFALTVSDLVWRAGPRTILDGLNLRLDGEGITVLLGPNGAGKSV
                     LLRHVCGLLRPDAGLIDWNGAATPGAGVSMVFQQPRMFRDSILGNVALALTPRGIAAT
                     ERRRRAAAVLERVGLAHRLTDSARGLSGGERQRLALARAWVTRPQLLLLDEPTASLDP
                     SSVEAIERIVREIRTDGTRILMTTHNLGQATRLADDVVFMDAGRVCEHAPVQRFFARP
                     ASPAARLYIQGELPWRMAF"
     gene            complement(14223..14927)
                     /locus_tag="PKX01_09055"
     CDS             complement(14223..14927)
                     /locus_tag="PKX01_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ76314.1"
                     /translation="MSGMGQAFLEAFQLLTGFDRQVVDIVVLSLRVSGLAVLAGTLVG
                     LPLGACLGVGRFPGRQALVIFLNACMGLPSVVVGVVVYLALSRSGPFGHLGLMYSPVA
                     MVIAQTLLVIPLMAAIARQVVEDAWRDYAEELAAMRFRWIDSVTLLLHDCRHSLLVAV
                     LAGLGRAMSEVGAVMIVGGNIDRSTRVMTTAIALETSKGDLPLALALGVVLLAVILLI
                     NAAAYMLRTWAFRRYA"
     gene            complement(14944..15249)
                     /locus_tag="PKX01_09060"
     CDS             complement(14944..15249)
                     /locus_tag="PKX01_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76315.1"
                     /translation="MVENVLAPEGFSREMSISMTEFERALRLAETDRTITVTAGRFRV
                     EGDGAVLDVSLTPLPDRVIGMIRLPALAAHLRFLAGNGAERRTLLHRIDLAMRRGGG"
     gene            complement(15258..15509)
                     /locus_tag="PKX01_09065"
     CDS             complement(15258..15509)
                     /locus_tag="PKX01_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HMZ76316.1"
                     /translation="MKIAFKLFASLADYLPAERRSNRVELDLAPGTTVGDLIDRYRIP
                     ERSAHLVLVNGHYIEPNARRSHVLAEGDELAIWPPIAGG"
     gene            complement(15506..16792)
                     /locus_tag="PKX01_09070"
     CDS             complement(15506..16792)
                     /locus_tag="PKX01_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ76317.1"
                     /translation="MKHLILGNGPAGVVAVEALRKADPRGEIVMVGSENEPPYSRMAI
                     PYLLEGNIEEPGTWLRKTPGHFDALGVRQLSGRAVALDVADRRVLFADGHFEHYDRLL
                     VATGSHPVRPPIPGVDLPEVQTCWTLEDARAIARLAKPGSRVLQLGAGFIGCIIMEAL
                     AKRGVQLTVVEMGDRMVPRMMTPQAGGMIKRWVENQGIRVVTSAGVERIDHGDGHGAP
                     LLVSLSTGDKLPCDLVIVAAGVAPNVSFLEATPVHVAKGVLVDARMETSVPGIFAAGD
                     VAEAPDLFTGAHLVSAIQPNAADQARVAAINMAGGQARLTGVLAINVLDTLGLISTSF
                     GQWWGDPEGQGVEQVDEVAFRYLSLQFKEDVLIGATSIGMTQHVGALRGMIQGRLRLG
                     PWKDVLLHAPQRFFEAYLAATQSDAIARHQPVAAFA"
     gene            complement(16919..18766)
                     /locus_tag="PKX01_09075"
     CDS             complement(16919..18766)
                     /locus_tag="PKX01_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HMZ76318.1"
                     /translation="MGWNRKILRVNLTAGTCKEEPLNMAWADEYLGSRGLATKYLVSE
                     IDPKVDPLSPENKMIMSTGPLTGTVASTGGRYTVVTKGPLTNAIACSNSGGFFGAEMK
                     FAGWDMIIFEGRSPKPVYLYVENDKAELRDASHLWGKSCWETEEMIKHAHQDPLVRVS
                     SIGLAGENQVLFSCIVNDLHRAAGRSGVGAVMGSKNLKAVALRGTQGVSGIKDMKAFL
                     KITAEKKKILADNAVTGQGLPKYGTQVLMNVINEIGALPTRNHRNVQFEDAGKISAEA
                     MHEKRPTDGKPHLVTNAACFGCTIACGRISEIDKGHFTVVNNPKYWGASGGLEYEAAW
                     ALGAANGVGDLEALQYANLLCNEQGMDPISFGATIGAVMELFEMGLLTKEQLGVEAPF
                     GSAAALAHLAEITAKGEGFGKEIGLGSKRLCTKYGHPELSMTVKGQEFPAYDARGIQG
                     MGLTYATSNRGACHLRSYTVASEVLGIPVKTDPLTTEGKPELVKAFQDATAVFDASGI
                     CVFTSFAWTVADVQPQVQAACEGDWSMDKLNAMGERIWNMERMFNLAAGLTHKDDDLP
                     PRLKTEAADSGPAKGLVSGIDKMLPEYYALRGWSEEGVPTASTLERLGL"
     gene            complement(18782..19198)
                     /locus_tag="PKX01_09080"
     CDS             complement(18782..19198)
                     /locus_tag="PKX01_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ76319.1"
                     /translation="MACSVEHTGIINPSKSRIKVFSFETEGRKVPYTCTQCTEAWCLN
                     ACPVDAIRVNGTTGAKEVHEDVCVGCKVCTISCPFGTVNYNQETGKVQKCDLCGGDPA
                     CASACPTGAITYIDADWTGLDRMRAWAAKANTAVTE"
     gene            19541..20797
                     /locus_tag="PKX01_09085"
     CDS             19541..20797
                     /locus_tag="PKX01_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HMZ76320.1"
                     /translation="MSQDSGCAAPSSSPASLTVDAARRLIFDRLTPVGGYERIALRAA
                     LGRILAEPVIAPCNVPAHDNSAMDGYAIRADDLPESGEASFQVVGTAYAGRGFSGIVG
                     AGQAVRIMTGAVVPMGADTIVVQEIVQTDGDRVVVPPGQRRGQNLRRAGEDLALGAIA
                     LPAGKRLGPAELGLVASLGIAEVSVTRRLRVAFFSTGDELASIGRPLAPGEVYDSNRY
                     TLFGALTRLGCDLLDMGVIRDEPESMETAFREAARHADVIITTGGVSVGEADFIRDLM
                     ARLGEVSFWKLEIKPGRPMAFGRLGSAWMFGLPGNPVAVMVTFYQFVQDALLHLGGLT
                     PLPERPLVPAECADAIRKQPGRREFLRGRLSREDGIWKVRLSGAQGSGILRSMSEANC
                     FIVLEETRGNVVPGDAVSVQLFDGLI"
     gene            complement(20901..>22462)
                     /gene="prpE"
                     /locus_tag="PKX01_09090"
     CDS             complement(20901..>22462)
                     /gene="prpE"
                     /locus_tag="PKX01_09090"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HMZ76321.1"
                     /translation="VLIYMPMIAEACFAMLACTRIGAIHSVVFGGFASHSLATRIDDA
                     KPTVIVSADAGMRGGRPVPYKHLLDEAINLAEHKPAKVLMVNRNLDPNMAVVAGRDVD
                     YAALREKHLDAQVPVTWMESSEPSYILYTSGTTGKPKGVQRDTGGYAVALAASMKHIY
                     CGNPGETFFSTSDIGWVVGHSYIIYGPLINGMATIMYEGTPLRPDAGIWWKICQEQDV
                     TVMFSAPTAIRVLKKQDPAFLKKYDLSKLRALYCAGEPMDETSHQWMMDELGIQVIDH
                     YWQTETGWAMLSLMPGVERQEVQLGSPGFPVYGYDVRLFRDDGSECGPNEKGIVGVVP
                     PLPPGCLTTVWGDDKRFVSTYFSLFQEPLVYSSFDWGIKDDAGYYKILGRTDDVINVA
                     GHRLGTREIEEAIQSHSAIAEVAVVGVADQLKGQMPMAFAVVKDPASVDTPEKVVELE
                     KAVMKIVDGSLGAIARPARVHFISGLPKTRSGKMLRRSIQALAEGRDPGDLTTIEDPS
                     TLEQIKSALTK"
BASE COUNT         3727 a         7545 c         7383 g         3807 t
ORIGIN      
        1 ggcagcgcgc cggcatcttc catggtgttg aagaagatcg gggcgatctt ggagcccagg
       61 cacacaccgc cgaagcgctt gttcgggacg aaggggatgt cttcgccggt gaaccacagc
      121 accgagttgg tggcggactt gcgggaggag ccggtaccga ccacgtcacc cacgtaagcg
      181 atcaggttgc ccttggcagc gagggcttcg agctgcttga tggggccgcg ggaacccggc
      241 tcatcggctt cgatgcccgg acgcgggttc ttcagcatgg ccagggcgtg cagcgggatg
      301 tccgggcggg accaggcgtc cggcgcgggg gacaggtcgt cggtgttggt ctcgccggtg
      361 accttgaaga cggtcagctt ctggacggcg gggacttcgg gacgggaggt gaaccactcg
      421 gcgtcggccc aggactgcat cacggccttg gcgttggcgt tgccggcctt accgagttcg
      481 gcgacgtcgt ggaagtagtc gaagaccagc agggtcttct tgaggccttc ggcggccacg
      541 gcacccactt ctgcgtcggc gagcaggtcg atcaggggct tgacgttgta accgccgagc
      601 atggtgccca gcagttcggt ggccttggcg cgggagacaa gaccgcaggc cagctcgccc
      661 ttggcgacct tggcgaggaa ctcggccttg accttggcag cgtcgtccac gccggcggga
      721 acgcgatagg tcagcagttc caccaggaag gcttcttcgc cggccggcgg attttgcagc
      781 agcgcaacca gatcctgggc ttgctttttg gacaggggca gcggcgggat gccaagggca
      841 gcgcgctcgg caacatgctg gcggtaggct tcaagcattg gtatgtcctt cctagacggt
      901 gggttgtgta tgaagtgatt gctgacttga gcgaccggcc gctggggccc gatgcgcctc
      961 tcccgatgtg accggacctt ttggaacatc atgcggtcct gagtcttata tatattatat
     1021 tttacttctt ccccggatgt gcgcctgcac aattcaaaaa tctgtccggt cccggctgtc
     1081 ccaccaatcg gggccggccg gtacgggaat tgccggatgc aacagcttct tcattcgcgg
     1141 agccgatcat ggtacgcctt tgacgacggg acggggatac ggcagggagc ggctcagccg
     1201 gtcagaacac tggccaggac cgcttccatg gcgcccatcg tgaccggctt gatcaacagg
     1261 tcgtcgaatc ccgctgccag gaggccctgc ttctcgtcgg gcagcgcatg ggcagtatag
     1321 gcaatcacgg gcaggctgcc gaagaccgcg tcgctgcgga tgcgggccag cacctcttcc
     1381 ccggacatgc cgggcatgct gatgtcgagc agcaccgcgt cgtagcggtt cgccgagatc
     1441 agggccaggg ccgtagcacc gtcggcggcc tggtcggacg tgatgccgag acgcttggtg
     1501 aaggccgcag ccagtgtacg gttgatggga ttgtcgtcca cgatcaggac atgtttgtcc
     1561 tggaatgtca gggggctgct catctcgggg aggcggtcgg gcgtgccgtt gtggcggcat
     1621 cgcggggaag taggagggtg aaggtggagc cgacgcccgg ttcggagcgc agggacagtt
     1681 cgccgcccag cagatggacg aactggcggg cgagtgccag gcccagacct gttccggcct
     1741 gttcgcgcgt gacgaaggcg tccacctgcc tgaacttgtc gaagatcacg tcctgggcgt
     1801 ccttgggtat gccgcgaccg gtatcggaga ccgccagcgc gatccgctcg ccgtcggcat
     1861 ggatgttcag atcgatcctg ccattgtcgg tgaacttgat cgcgttgttc aagagattgt
     1921 tcaggatctg gcgaagacgc agcgggtcgg tgtggaggcg ctccggcgta ccggcggcga
     1981 tcgtgcattc gagttgcagc cccttggcct gggcgtgacc acggtgggcc gcgcaaacct
     2041 cctgggcgaa ggtcgcgagg ggcacgtcgc tgaggttgag ctccatgcgc cccgcctcca
     2101 ccttggccag atcgagcaga tcattgacca gatccagcag gtgctggccg gaggactcga
     2161 tcgtggcggc gtactcctga tgggcggcgt cggtggcatc gagcttgagc agttccgcaa
     2221 agccgaggat gccgttgagg ggggtccgca gttcgtggga gatgttggcc aggaactcgg
     2281 acttcatgcg gttcgcggcc tcggcgctct ggcgggcgtc ttcggatcgc ttgaaggact
     2341 cctgcacgcg gtcggccatg ctgttgaagg attcggccag gtgtcccatc tcgtcggtgg
     2401 atttcacctc cagccggcgg ctcagatcgc ccgcctgaaa accgtccagc ccttcgatca
     2461 tgcgggtgat gcgacgggtg atgaactggg ccatccagat cgcgacggcg atcaccaccg
     2521 ccaccatgac gagcgtggag atggccaggg ctccggcggt ctgggtcaga ttgtcgttga
     2581 tggcctggct gatgccggcg cgcagcgtct ggaattcctt gtccttggcg tcgatggtca
     2641 ggccgatgcg tttggcggat tcggtggcgg cacgatggaa gtcatccaca ttggcgccga
     2701 tggtgacgat tccaaaaccc tggggcgacg cggcatacgg tccggtgtaa tacggaatgg
     2761 cggccgctgt ggtgagcttc cacagcccgg aaaagaagat cacgaacgaa ccggagccac
     2821 ccttctcggt gagcagcttc cagccggaac attgaggcga gaagttcagg tagcggcagt
     2881 cgagcgccac cgtcccggcc ttgaccaggg cacccgcagg cttcttcttc agggactgat
     2941 cgtcgtaggg cttcacccct tcaaggaagg cctgtgacga ttttccggag gactgccagg
     3001 cttcgtacag ggactggtcc atccaaggcg taaccggctc cccggtggcc ggatcgtagc
     3061 cgggaatgaa gtagtcgcgc gggtgggaaa tggcacggcc acgatggtcc cacatgaagg
     3121 catagttgcc ggcgatggcg tcattgatcg gcgtatagcg cgcatccgtc gggctgatcc
     3181 ggtcggtgaa ctggcggatg tggtcgtggt cgagggccag ggtcacgtag ccggtgatct
     3241 gtccttgacg caccaccggc atggcccacc gcacgatgcc gcgaaaatgg cgccccacgg
     3301 gattctcggt cccggcgtag gccgacgctt cgggcttgaa cggctcgccc gccttctccg
     3361 cagacgccgg cagatagggg ccgatcacgt ggctcttcac ataggcgccg acgacatccg
     3421 acacatggat ctcgccgggc ttgagcttct gcagttcagg ccagtaatgc tccgcccgga
     3481 tgaaggtctc gcgggcgtcg ctgacctgac gcagccccgg cgtcatcagc gaaccctggg
     3541 tcaccttgac acgctcccga ccggacaggt cgatgaagct catctccacg aagagcgggc
     3601 gcatctcccg atggccgagg aactccggcg ggcgggcgct gaaggctttg gcgttgtcag
     3661 agagggcctg cccggcgtcg gccgcaaggg attcgtccca ggattccggt gctgcgggct
     3721 cccagcgttt gccgtcatcc gcgagtttcc aggggccgtg ggaaaatacc gggcgctgcc
     3781 gatgcgcgag gaagcgtcgg tagcttgcgt caccgggctc cagttcggcg gcttcacgga
     3841 tgtccgcgtc ccggtcgtaa aggaacgccg ccacggcgcg ggccgtatcg gtggtgaggc
     3901 gctccatgct ttcccgcgcc cggtcgtcga gcgcgtgcag cgcatcctga gtcgcctcct
     3961 cgcccacgct gcgcaaggta cgcagcatcg agctggacac gctctccagc tggaggccga
     4021 ccgcatcgcc cagcttctcg gccattgtcc aggccaggag ggccaacagc accagcggca
     4081 cgaccttgat gacgatgaag atcgagatca gtttgccccg gataccgaat ctgcccgtgt
     4141 tggtctgcat ggtcttcccg tgtccgtttc catccatcgc gccgtcacca ggcgcgcgcc
     4201 tgggtgcccg acgtgcacat cggctgtccg tccgaaatat caagatcgga tctcaagaaa
     4261 gtttgagcgc cttgcccgcc gtcccagggg cgccgacaat gtcgggtcgt cattccgctc
     4321 acccccatgt ccgacccctg cccttgcggg tccgggcgcg cctacccggc gtgctgtggc
     4381 ccctacctgg atcagtcact gcccgcgccc acggccgagg cgctgatgcg cgcccgctac
     4441 accgcctaca ccctcaagcg ggaggactac ctgctcgcca cctgggaccc ggcgcatcgc
     4501 cccgcgcaac tcgatctggc ctctgacacg gcaacccgct ggctggggct gcaggtgaag
     4561 cgtcatgaga ccaccggatc cgggcaggcc atcgtcgaat tcgtggcccg ctacaaggtc
     4621 gccggccgcg cctaccggct gcatgagacc agtcgcttcc atgtgctcgg cggtgcctgg
     4681 gtttacgtgg acggggacgt ccactgacgg gcggtacgtt caggtcgcca cgagctcaat
     4741 cacctcgccg gcaaattcga cccgctggcc cggacggagc ttggcgcgct tgcgggtctc
     4801 gatgacgcca tccaccttca cctgcccttc tgccacacgc tgcttggcga cgccgccgga
     4861 atcgcacagg ccggtggcct tgaggagctg atcaagctgg atgaaatcgc cctgaacggg
     4921 gaaacgggtg gccatggcac ggacgtccaa cgtcaaaggg gcggattgtc gcagagcctg
     4981 ccgcccggcg ccaatccgcc tgccgggtcg aatgcctgcc gatcaggtgg ggatccgcag
     5041 catctggccc ggatagatct tgtccggatg tcccagcatc ggccggttgg cctcgaagat
     5101 cttcatgtac gcgttggcgt tgccatagta gtgcttggcg atggccgaca gggtgtcgcc
     5161 gcgtaccacg gtgtggaact gggcttccgg ttccgggtgg gtgacggcga gctggtcgat
     5221 cacctgctcc accccgtcca cgttgccgca gcacagcagg atcttctcct tggtggcctg
     5281 atcggacacg ctgccactcg cggtgacggt gctggtcgcg ccgtcgaagc tcacgctcag
     5341 tgcgctggcg tcgagaccct gggtgccgat gtaggcaagg atcgccgcgg cggccttggc
     5401 gttcaggtcg gccaggcgtt ccgccgcgcc ggaatcggcc gccgctgctt ccacgtcctt
     5461 gcggccgaac agcttttcac cggcttcacg aacaaaactc aaaagaccca tgacggactc
     5521 cctgcgtggt gtaagaaagt ccccagccta cggcctcgga cgcacacggc ggatgtcgag
     5581 agtgtgacga atcgtgacgc cgcgctcagg cccccagcgg gggcaggcca tgtcggcggc
     5641 gggcgcgggc acagccttcg tcgccgatgc caagggccgc gtagggcgcg aaatcccggc
     5701 agggccccgg ccgctgttcg tagatcgagc acgacacctc gcgcccgacc tcgccgctca
     5761 gggcgacgca gcggggcgga ccgtcatccg tgccgcgcat gcgcaccagg gtggcggtga
     5821 ccggcacagt cagcgcgtcc ggcacaccgc cgccgggatg gctggccaga tccgacacat
     5881 gaaaatcgac gcgaaaactg gcgcaacagg caccgcagct caggcaggga tgggacatgg
     5941 gagctcaggt cgccccgcag catttcttgt attttttgcc gctgccacag gggcagggct
     6001 cgttgcggcc ggggccggcc ggcgcggcac ctgcggcggc ggccaccaca acgggttccg
     6061 gggccgccag tccgagggca cgccaggtct ggaccagacg atggacggcg atgcccttcg
     6121 cttcaaggat gtccagctgt tccgcgctgt cggccactgc atcctcctcg tcgtcaccca
     6181 gcagggtttc gagcagagcc tcggcgatgc tggtgtcttc cacggtgtcg gtccattcgg
     6241 tcggccacag ctcaaagccc tgttcgaatc cggcggtcca ttcctcgccg atggccgggt
     6301 cgtcgtctcc accctgggca tagcagaacg gctgccaggc atcgtccgcc tcatcgtcgc
     6361 cgatcgcgag cagaagatcg ttgtgataac gcaggaccag ttcgatggct ttctgaagta
     6421 cggccccgga accgaaacct gcctcgtcgc cgtcagcggt ccacactgcc ggcagccaga
     6481 cctctaccgg gatcggtccc ggcgctgcca cgacggccgc caggtagccg tccagcatct
     6541 ccatgttcat gcaatccgcc gggacgtcgt cggaagccag cagttcttca agctgctcca
     6601 gttcttcttc ggtcaggggc atgctgacgg gaatcgggtt catgacgact gttcctccag
     6661 ttggtcgagc tccaccacac actggtatag cgtctggccg gaatcccggt atttgcgttc
     6721 gaaaggggtg agcggcgacg gcgcctcgag gggctgccag ggcgccggca caccggtgag
     6781 cagctccacg gcgcgggcga attccgcgat gtaaatcggc cagttgctgc ggcactccag
     6841 gcgcccaccc agacgcggca cgcacggaaa caccgggtgg gcgtgccagc gccgggtgag
     6901 gtgtccgatc ttcggccacg ggttgggata gaggatgtaa tggcggtcca ggcgaatgcc
     6961 cgcatcggcg agcagacgcc agtagtccac cagatcggca cgacagaaca cgagattggt
     7021 cggcatcagc gcgtccggat agggcttctt gcggctcagg cggtcctcgg actgatccac
     7081 cccgatcaca taatggtcgg gaaaagcccg cgccagggcg atggtgctgt gccctactcc
     7141 gcatccggcg tccagcagca gcggcgcgcg ctgatcccag ccggcaagac tggcttcaaa
     7201 cgcggccacg ttgtagggcg catagggttt gcggaagtcc gtcgcggcgt gcttgttcac
     7261 gcggctctca agggcctcgt ggagtccgct ctgggcgctc tcggggatgc gggaattggc
     7321 gtacatccgc cgattctacg cggctgccga cagcaccggc accccgtggc gccgcagatg
     7381 gccctcgacc gccgtgcagt ccttcaatgc gcggatctcg ccatacaagg actccgcctg
     7441 cgggaagttg cggcgcagca ggttgagcca ctgcttgagg cgtcccggcg catggcgggc
     7501 ttccagcttg gcgcgtaccc gccaccagaa ttccccgagg agcggcgaca gctcggccca
     7561 ctcggcgtcg cggtcggggg ttttggtgcc gtcagcctgc agcgcgcgga tgcgccgtgc
     7621 cagaaagggg tcggccaccg cgccacgccc cagcatcacg tcgctcaccc cgctcacggc
     7681 acggcagcgt tgccagtcgg cctcgctcca cacttctcca ttggctgcca ccggcacgcc
     7741 gaccgtatcg gcgatgcgcc cgacccattc ccagtgggcc ggcggccgat agccgtcgac
     7801 cttggtgcgg gcatggacca ccagcgcgtc gatgccgccg tccgccaggg cgcgggcgca
     7861 gtccagcgtc cgccccgtat cggcgatgcc caggcgcatc ttggcggtga agggaattcc
     7921 cttgggcacg ctcgcccgga ccgcgcaggc aatggcgtgg agcagttccg gctcgtcgag
     7981 gagggcggcg ccgccccggt gacgattcac cgtgggcgcc ggacagccga agttcaggtc
     8041 gatgcccgcc ggatgcagac gcaccaggcg cgccgcgctc gccgccatac gctgcggatc
     8101 ggagcccagc agttgcaggt ggaccggcgt gccggacgag gtccggctgc cgactttcag
     8161 ctcgggcgca atacgggtga agctgcgccc tggcaacacg gtcgccgaga cccgcagaaa
     8221 ctccgtcacg gcatggtcgt agcccgggat gcgggtcaac acctcgcgga ggacatcgtc
     8281 cgcaaggcct tccatcgggg cgaggtaaag gcggggcatg ggcaatcggg tccggcgtca
     8341 cggccgggcg ctgcgagaac aggggcggca ctttaccgga gttggctcgc ggactgaagg
     8401 ggccgttccg tcgcgcgcac gggcctcggc aaaggggtat agtcacccgc ctctgtgtat
     8461 cccgaccgtc cgcctgcgca ccctgcccat catgcgccga attgacttca caaatcgccg
     8521 ggaatggctg accctggcgc tccccgccct gctcctcgtc gtcgcggcct ttgcggtggc
     8581 gtggaagttt gtccagcccg ctccgccccg acagatcgtc atgagtaccg ggcccgccgg
     8641 cggcgcctac cagtactttg ccgaacgcta tcgggaacgt ctggcgcggg atggcatcga
     8701 actggccctg cgtccgtcca ccggcgccgt ggagaacctc aaccgcctca agaccgtgga
     8761 tgaggtggac atgggcttcg tgcagggtgg cgtggccgac gagccggagt cggacgatct
     8821 ggccaccctg ggcagcatgt acctggaacc cgtgtgggtg ttcttccggg gcaaggccga
     8881 tctggaccgc atcgaccagc tgaagggacg ccgcatcgcc gtgggggccc cgggcagcgg
     8941 aacgctcctg ttcgcactcc agttgctgtc cgccaacggc tttgcgaccg atgatcctca
     9001 tttcatccac ctgggcgggc tggaggccgt cgaggcgatc cgctccggtc aggtggatgc
     9061 agtatttgcc gtgggggccc cccaggcccc ggccatccag gccctgctcg ccgagccagg
     9121 cctgtccctg ctcaatttcg cccaggccga cggctacgtc cgccactttc cgcacctgtc
     9181 ccgcatcacg ctgccccgcg gcgcgatcga cattgcccag gatcgcccgc cccgcgacat
     9241 ccacctcctc gccgccaccg cgaatctggt ggtgatggcg gacctgcatc cggcgatcgt
     9301 cacccgcgtg cttcagcatg ccgcgcagat ccatggccag cccggcatgt tcatgacgcc
     9361 cttcgccttt cccgccaacc aggaccgctc cctcccgctg catccttctg cgcggcgctt
     9421 ctacgaaagc gggccgccct tcctgcagcg ctacctgccg ttctggctgg cggtgctgat
     9481 cgatcgcctt atcgtgatgc tggtgcccat cgtggcggtg gtgattcccc tcttcaaggt
     9541 gctgcccgcg ctctacaact ggcgcatgcg ctcgcgggtg tatcgctggt acggcgaact
     9601 caagttcctc gaaaatgaga tcggccagca aagcacccac acaccggaag tgctggatga
     9661 cttcatccgc cgcctcgatg ggatcgagga ccgcgccagt cgccggaagc tgccgctggc
     9721 cttcagcaac gagctctaca ccctgcgcga acacatcaat ctggttcggc agcgccttga
     9781 acgcctgcag gcacgggctc aagtccctgc ggcgccgacc gtttcatgaa tccatccttg
     9841 ccgtccggaa cctcacccgg atccacattg accgcgatct gcccatgagc ccccacctgg
     9901 acctcaccga accctacgcc ccgctcgtcc gccgcatcga agcggaggcc accgtcacca
     9961 ccgacggcaa tctgcgcatc gaccactttc tcaaccaccg cgtcgaaccc gcctacatcg
    10021 cagagctggg gcacgagctg gcccggcgcc tgtcctactt ccagcccaac gtgatcctca
    10081 ccgccgaggc cagcggcatc gcgccagcct tgatcgtggc ccaggtgctg agcatcccca
    10141 tggtgtacgc caagaagtac tcctgcgacg tggagggccc cgccatcagc cggatcgtgg
    10201 cctcgccggg acggggcgcc gaagttcgtc tggccctgtc gtcccgctac gtgcgcagcg
    10261 gacatcgggt cgtggtcgtg gatgacttcc tgtccaacgg ccgcacggcg gcggccctcg
    10321 tggagatggc ccgcgaggcc ggcgcagagg tgctcggcgc cggcttcatc gtggagaagc
    10381 gcttcaatcg cgggcgcgaa catatcgcag caatgaacgt ccccatcgcc accctcgccc
    10441 agatcgaatt cctgcacgac ggccgcgcct gcctgaccca gccgcccgcc gcctgagcgc
    10501 cgccatgtcg ccccggcaga tcctgcgcag cctcttcgac gccgccctcg ccgcggccca
    10561 gcccgagcgc gtcgtgccgc ttcatctgcc agacggcggc gccctgcccc gcgggcgcgt
    10621 catcgtcatc ggcgctggca aggcctcggc tgccatggcc cgggccgtgg aagatcactg
    10681 gccgggtgat ccggctcgac tgtccgggct ggtcatcacc cgctacggac acggggtgcc
    10741 ctgcaggcat atcgaaatcg tcgaagcggc ccacccggtg cccgacgaac gcggctacgc
    10801 cgccgcccgg cgcatgctcg aacacaccgt cgcggagctt ggcccggacg atctcgtgct
    10861 gtgcctcatt tccggcggtg gctctgccct gctggcggtg ccagccgcgg gcatcaccct
    10921 ggccgacaag caggccatcg gccgcgccct tctcgccagt ggcgccacca tcggcgagat
    10981 caactgcgtg cgcaagcatc tctcggcgat caagggcggg cgcctggcgc tggcctgcca
    11041 gccggcacgg gtgctgaccc tgctgatatc cgacgtgccc ggcgatgacc cgtccatcat
    11101 cgcgtcgggc cccacggtgg gagacccgac cacctgcgcc gaggccctga ccgtcctcga
    11161 caagtacgcc atcccgctgc ccgcgcatgt ccgtgcgcac ctcgaatccg ctcgcggcga
    11221 aagccccaag cccggtcatc cggcctttgc ccggtgcgaa acccgcctca tcgccacgcc
    11281 gcagcaggcc cttgaagccg ccgcccgaag ggcccgcgag ctggggttga gcgcccacat
    11341 tctgtcggac gccatggaag gcgaatcccg tcacgtcgcc cgcgtgcatg ccgccctggc
    11401 gcgccaggtg gcggaccgcg gccagccctt cgtggcaccc tgtgtgctcc tctccggcgg
    11461 cgagaccacc gtcaccgtca gcggcaaggg ccaggggggc cgcaatgtgg agttcctcgt
    11521 ggccctggcg caggccctcg acggccaccc gcgcgttcat gccctggcgg ccgacaccga
    11581 tggtgtcgac ggcagcctcg acatcgccgg tgcctgcatc gatccgacga cgccggcccg
    11641 caccttagcg ctgggacggc gtctggccga cgtgctggcg gacaacgacg gtcacgggct
    11701 gttccaggcc ctgggcgact cggtcgtcac cgggcccacg cagaccaacg tcaacgattt
    11761 ccgtgccatc ctgatcgacg cgccggacgc ggcactctga gcgttcgcgc cccgacgccg
    11821 gccgcggatc agcggaaatg cacccgcact tccggattgc cgggcaggcc atcgtaacgc
    11881 accacaatgt cgagaccgga tttcggcgcc aggaggcggc tgaaggagcc cagggacagc
    11941 agatcgcccc gctgcagcgg tgtgccggcg gcggcgagat cccgggccag ccagatcgcc
    12001 gcattgagcg gatgacccag gatggcgctg ccctgggcgc tgtccagcac ggcccccgtg
    12061 ccgtcccgta ccgacacagt cattgacgcg agcgcctccg cccggagacc gcccgaccgg
    12121 gccggcacgg acgtcccctg cacgccaaga cgcgcgccca cattcaccag ggccagcatc
    12181 ggcccactca gtgcgcccgg atcgccgacc atcagatccg gcaactccac gaacgggatc
    12241 accgcggaga gggcctgcaa ggccgcgtcg ggcgatccgg cccgcgccag tgcatgagga
    12301 tcggccactt ccaccagcag gtccgcctcg aagaccgggc ggataccgaa cgcggccggc
    12361 acctgggcac cgtcgggtag cagaccggca cggaacaggc gaccccgcag cggggccccg
    12421 tagccgaagc gctgctgtac cgccgggttg gtgagccccg ccttgtgccc gaccggtgct
    12481 ccccaggcct gggccaggaa gcgcaccacc ttgtcccgtc cgcacagacc gtcctccatg
    12541 cgcgtgagac cgggcaccgc gtgtacgggg gttcggttca cgatggcggc tgccaccgca
    12601 gcggcctcca cgtcagcagg acattccgcg cgggcgccgg acaccagcag cgcgcccatc
    12661 agtacgccgg cccgccagcg cgaggacccc cggcacatct cagcgcgcca gcgggaagaa
    12721 aagctgctca ttgccgatct tgtaggcagc gatggctcgg cgtccgtcgg cggaggtcag
    12781 ccagtccatg aagcgcagcg ccgccgcccg cttgatgtgg gggtgcctgg cgggattgac
    12841 gaggatcacg ccgtaggggt tgtacaactg cggatcgccg gcgaacagga tgcccagatc
    12901 ctgacggttc cggaagctgg cccaggtgcc ccggtcgctg agggtatagg cgcccaggcc
    12961 tgccgccatg ttgagcgtcg gacccatgcc ggcgccggcc tccttgtacc agccctggcc
    13021 cctgggatcg accccggccg ccttccagaa gcgcagctcg gccgcatggg taccgctgcg
    13081 gtcgccgcgg gagatccagg ttgccccctg actgccaatc cgtgcaaagg cgtccgaagc
    13141 atccctggca cgcttcacgc cggccggatc ggcggccgga ccgatgacta cgaagtcgtt
    13201 gtacatcaca tcgcgtcggt cgacgccgta gccgtcggca acgaacttgt cctcggccgg
    13261 tcgatcatgg accaggacca cgtccgcatc gccccgccgc cccacgtcga gggcctgccc
    13321 cgtgcccagg gccacgaccc tgacggcgat gccggagtcc ttttcgaaga tgggcaggat
    13381 gaagttgaag agaccggatt gctccgtcga cgtcgtcgac gccaccacga tgctttcacc
    13441 ggcctggacc gctgccgtgg acaccgccag tcccagcagg accgaacgga aaaatttcag
    13501 aaggccatgc gccatggcag ctctccctgg atgtaaagac gtgccgccgg gctggcggga
    13561 cgcgcgaaga aacgttggac cggcgcatgt tcgcacacgc gaccggcgtc catgaagacc
    13621 acatcgtcgg ccagccgggt ggcctgaccg aggttgtggg tggtcatgag tatgcgcgtg
    13681 ccgtccgtac ggatctcgcg cacgatgcgc tcgatggcct ccacggacga ggggtcgagg
    13741 ctggcagtgg gttcgtcgag caacagcagt tgggggcggg tcacccacgc ccgcgccagc
    13801 gccagccgct gccgctcgcc gccggagagc ccccgtgcgc tgtccgtcag gcggtgggcc
    13861 agaccgaccc gctccagcac cgcagcggcc cggcggcgac gctcggtcgc agcgattcca
    13921 cgcggtgtga gggccagcgc cacattgccc aggatcgaat cgcggaacat gcgcggctgc
    13981 tggaagacca tggatacccc ggcacccggc gtcgctgcgc cgttccagtc gatcagcccc
    14041 gcgtcgggtc gaagcaatcc gcacacgtgc cgcagcagga cgctcttgcc ggcaccgttc
    14101 ggtcccagca ggacggtgat gccctcgccg tccaggcgca ggttcaggcc gtcgaggatc
    14161 gtgcgcggcc ccgcccgcca tacgaggtcg ctcaccgtca gcgcaaacat cggcggacgt
    14221 cctcaggcat agcgccggaa tgcccaggtg cgcagcatat aggcggcagc attgatgaga
    14281 aggatgaccg ccagcaggac gacgcccagg gccagcgcga ggggcaggtc acccttgctg
    14341 gtctccaggg cgatcgccgt agtcatgacg cgggtcgacc ggtcgatgtt gccgccaacg
    14401 atcatcactg cgcccacttc ggacatggcc cgtccgagcc cggcgagcac ggccaccagc
    14461 agggaatgcc ggcagtcgtg caacagcagg gtcacgctgt cgatccagcg aaaacgcatg
    14521 gccgcgagct cttcggcgta atcccgccag gcatcctcca ccacctggcg ggcgatggcg
    14581 gccatcagcg ggatgaccag gagggtctgg gcgatcacca tggccaccgg ggaatacatc
    14641 agcccaagat gaccgaaggg gccggagcgg gacagggcca gatagacgac cacgcccacc
    14701 accacagagg gtaagcccat gcaagcgttg aggaagatga ccagtgcctg acgccccgga
    14761 aaacgtccca cccccaaaca tgcgccaagc ggcaggccga cgagcgtacc cgccagcacc
    14821 gccaggccac tgacccgcag cgacaggacg acgatgtcca cgacctgcct gtcgaaaccg
    14881 gtcagcaatt gaaaggcttc gagaaacgcc tgccccatcc cgctcatcca ggtgcgtgcg
    14941 ccctcatcca ccgccccgac gcatggccag atcgatgcga tgcagcaagg ttcgccgttc
    15001 cgcaccattg cccgcaagaa agcgcagatg cgcggccagg gccggcaggc ggatcattcc
    15061 gatgacccga tccggcagcg gtgtcaggga cacatccagc accgccccgt cgccttccac
    15121 acgaaagcga ccggcggtga cagtgatcgt ccgatccgtc tcggcgagcc gcagcgcccg
    15181 ttcgaactcc gtcatgctga tgctcatctc gcgggaaaaa ccctccggcg caagaacgtt
    15241 ctcgaccatg cgatccttca gcccccggcg atgggcggcc agatggcgag ttcgtccccc
    15301 tcggcgagca cgtgactgcg ccgtgcgttc ggctcgatgt aatggccgtt gaccagcacc
    15361 agatgagcgc tgcgctcggg aatccggtag cggtcgatca gatcgcccac cgtcgtcccg
    15421 ggcgccaggt ccaactcgac ccggttgctg cgccgctcgg ccggcagata gtcggcgagc
    15481 gacgcgaaca gcttgaacgc aatcttcatg cgaaggctgc gacgggctga tgacgggcga
    15541 tggcgtcgga ctgggtcgcc gccagatacg cttcgaagaa gcgctgcggc gcgtgcagca
    15601 ggacgtcctt ccaggggccc agccgcaaac gaccctgaat catgccgcgc agggcaccca
    15661 catgctgggt catgccaatg gaggtggcgc cgatcaggac gtcttccttg aactgcaggc
    15721 tcaggtagcg gaacgccacc tcgtccacct gctccacccc ctgcccttcc ggatcgcccc
    15781 accactggcc gaaggacgtg gagataagcc ccagggtgtc gagtacgttg atggccagca
    15841 cgccggtcag ccgcgcctga ccgccggcca tgttgatggc ggccacccgc gcctggtctg
    15901 ccgcattggg ctggatggcg gagaccaggt gggcgccggt gaacagatcc ggagcctcgg
    15961 ccacatcgcc cgctgcaaag atgccgggca ccgaggtctc catgcgtgcg tcgacgagga
    16021 cgcccttggc tacgtgaacc ggagtcgctt cgaggaaaga gacgttcggc gcgacacctg
    16081 ccgccacgat gaccagatcg cagggcagct tgtcaccggt ggacaggctc accagcagcg
    16141 gcgcaccgtg accgtctcca tggtcgatcc gttcgacccc ggccgaagtc accacccgga
    16201 tgccctggtt ctcgacccag cgcttgatca tgccgcctgc ctggggcgtc atcatccgcg
    16261 gcaccatccg gtcgcccatt tccaccacgg tcagctgcac gccccgtttg gccagggctt
    16321 ccatgatgat gcacccgatg aagcccgccc ccagctgaag gacacgggag cccggcttgg
    16381 ccagtcgggc aatcgcgcga gcgtcctcca gggtccagca ggtctggact tccggcagat
    16441 cgacacccgg gatcggtggc cgcaccggat gagaaccggt cgccaccagc agacggtcgt
    16501 aatgttcgaa atggccatcg gcgaagagca cccgtcgatc cgccacatcg agggccaccg
    16561 cacggccact cagttgccgg acgccaagcg cgtcgaaatg ccccggcgtc ttgcgcagcc
    16621 aggtgccggg ctcttcgatg ttgccttcca gcagataggg aatggccatg cgggagtacg
    16681 gtggttcgtt ctcgctgccg accatcacga tctcaccacg ggggtcggcc ttgcgcagcg
    16741 cttcgacggc gacgacgccg gccgggccgt tacccagaat caggtgtttc atggggaatt
    16801 ccttccgctc gtgcacagac gcgcgctcgc cttttggcaa gcggcgctgc gatgtatacg
    16861 tgaaaaggcc tctcccgccc ggcggggcgg gagagccaga acctggagat gctgcggctt
    16921 acaggcccag gcgctccagg gtcgacgccg tcgggacgcc ttcctcgctc cagccgcgca
    16981 gggcgtagta ctcgggcagc atcttgtcga tgccgctgac gagccccttg gccggaccgg
    17041 aatccgcggc ttccgtcttc aggcgcgggg gcaggtcgtc gtccttgtgg gtcagaccgg
    17101 ccgccaggtt gaacatgcgc tccatgttcc agatgcgctc gcccatggca ttgagcttgt
    17161 ccatggacca atcaccctcg caggccgcct ggacctgggg ctgcacatcg gccaccgtcc
    17221 aggcaaagga cgtgaagaca cagatgcccg aggcgtcgaa caccgcggtg gcgtcctgga
    17281 aggccttgac gagttcgggt ttgccctccg tggtgagcgg atcggtcttg accggaatgc
    17341 ccagcacttc cgacgccacg gtgtaggaac gcagatggca ggcgccccgg ttggacgtgg
    17401 cgtaggtcag gcccatgccc tggatgccgc gcgcgtcata ggcggggaat tcctgcccct
    17461 tgacggtcat ggacaattcg ggatggccat acttggtgca caggcgcttg gagccgagac
    17521 cgatctcctt gccaaagcct tcgcccttcg cggtgatctc cgccaggtgc gccagggcgg
    17581 cggccgaccc gaacggtgcc tcgacgccca gttgctcttt ggtcaggagc cccatttcga
    17641 agagctccat cacggcgccg atggtcgccc caaaggaaat cggatccatg ccctgctcgt
    17701 tgcacagcag gttggcgtac tggagcgctt ccagatcacc cactccgttg gcggcaccca
    17761 gtgcccacgc ggcctcgtat tccagcccgc cggaagcacc ccagtacttg ggattgttca
    17821 ccaccgtgaa gtgacccttg tcgatttcgg aaatgcgccc gcaggcgatg gtgcatccga
    17881 agcaggccgc attggtcacc agatgcggtt tgccgtccgt gggacgtttt tcgtgcatcg
    17941 cttcggcgga gatcttgccc gcgtcctcga actggacgtt gcgatggttg cgcgtgggca
    18001 gcgcaccgat ctcgttgatg acgttcatca gcacctgggt gccgtacttg ggaagcccct
    18061 gacccgtcac ggcgttgtcc gcgaggatct tcttcttttc ggccgtgatc ttcaggaagg
    18121 ccttcatgtc cttgatgccc gacaccccct gggtaccgcg caaggcgacc gccttgaggt
    18181 tcttggagcc catcacggcg ccaacgccgg agcgtccggc cgcccggtgc aggtcattga
    18241 cgatgcacga gaacagcacc tgattctcgc cggccagacc gatggatgac acacgcacca
    18301 gcggatcctg atgggcatgc ttgatcatct cctcggtttc ccagcagctc ttgccccaca
    18361 gatggctggc gtcccgcaac tcggccttgt cgttctcgac atacaggtac acgggcttgg
    18421 gcgagcgtcc ttcgaagatg atcatgtccc aaccggcaaa cttcatctcc gcgccaaaga
    18481 agccgccgga gttggagcag gcgatggcat tggtcagggg gcccttcgtc accaccgtat
    18541 atcggccgcc cgtggaggcg accgtaccgg tcagcgggcc ggtggacatg atcatcttgt
    18601 tctcgggaga gagcggatcg actttgggat cgatttccga gaccaggtat ttggtggcga
    18661 ggccacggga gccgaggtac tcgtcggccc atgccatgtt cagcggttct tccttgcagg
    18721 tgccagcggt caggttgacg cgcaggatct ttctgttcca gcccatgatg cgtctccttc
    18781 cttattccgt cacggccgta ttggcctttg ccgcccaggc ccgcatgcga tccagaccgg
    18841 tccagtccgc atcgatgtag gtgatggccc ccgtcggaca ggccgacgca caggccggat
    18901 cgccgccaca caggtcgcac ttttgtacct tgccggtttc ctggttgtaa ttgacggtac
    18961 caaaggggca cgagatggtg cagaccttgc accctacaca gacatcctca tggacttcct
    19021 tcgcgccggt cgtgccgttg acgcggatcg cgtccaccgg acaggcgttc agacaccacg
    19081 cttcggtaca ttgggtgcag gtgtatggca ccttgcgacc ctccgtctcg aagctgaaga
    19141 ccttgatgcg ggacttggac ggattgatga tccccgtatg ctcgaccgaa cacgccattt
    19201 cacactgcag gcaaccggta catttcttgg gatcaatgtg cagtgatttc cacattgaaa
    19261 acttcctccg tgatgatggt gctacgtgcc gggcggtgcg atcacatgtg gatcggagct
    19321 cacttcgagg tggcatgtac tgccgccggt aaggggtttc tcccaccaga cgcacagccc
    19381 gcttctccgg gacgtacgcc gatacgttaa agcatatcgt tggacgtctt cttgtcaatg
    19441 cgcgccgcac catgggctgc gccgattcac gatccggacg gatatgggat actccggccc
    19501 gacatgcggc gacgtgccgc gctgacgaag gactaccaca atgtcccaag attctggctg
    19561 tgctgccccg tcttcctcgc ctgcatccct gacggtcgat gcggcccggc gcctcatctt
    19621 tgaccgcctg acgcccgtcg gcggttacga gcgcatcgcc ctccgcgccg ccctgggccg
    19681 gattctggcc gaaccggtga tcgccccgtg caacgtgcct gcccacgaca actcggccat
    19741 ggacggctac gccattcgag ctgacgatct cccggaaagt ggtgaggcat ccttccaggt
    19801 ggttggaacg gcctacgcgg ggcgtggatt ttcgggcatc gtcggcgcgg gacaggcggt
    19861 ccgcatcatg accggcgcgg tcgtgccgat gggcgcggac accatcgtcg tccaggagat
    19921 cgtgcagacg gacggcgacc gggtcgtcgt gccgcccggt cagcggcgcg gccagaatct
    19981 gcgtcgcgcg ggggaggatc tggcgctcgg ggccatcgcc ctgcccgctg gcaagcgcct
    20041 ggggccggcc gaactggggc tggtggcctc tctgggcatc gccgaggtca gcgtgacacg
    20101 gcgcctgcgg gtggccttct tctcgaccgg cgacgaactg gcgagcatcg gccggccgct
    20161 ggcacccggt gaagtctatg actcgaaccg ctataccctc ttcggcgccc tcacccgcct
    20221 cggatgcgac ctgctggaca tgggcgtgat ccgggacgag cctgagtcca tggagaccgc
    20281 cttccgggaa gcggcccggc acgcggacgt gatcatcacg acgggcggcg tttcggtggg
    20341 cgaagccgac ttcatccgcg acctgatggc gcgtctgggc gaggtgagct tctggaaact
    20401 ggagatcaag ccgggccgcc ccatggcctt cggccggctg ggttcggcct ggatgttcgg
    20461 actgccgggc aatccggtcg cggtgatggt caccttctat caattcgtgc aggacgccct
    20521 gcttcatctc ggcggactca cgccgctccc tgagcgtccg ctggtgccgg cggaatgcgc
    20581 cgatgccatc cgcaagcagc cggggcggcg ggaattcctg cgcggacgcc tcagccgtga
    20641 ggacggcatc tggaaggtgc gtctgagcgg tgcgcagggc tcaggcatcc tgcgctccat
    20701 gtctgaagcc aattgtttca tcgtgctgga agagacccgc ggtaatgtcg taccgggcga
    20761 tgcggtctcg gtccagctct tcgacggatt gatctgagca gacacggccg gtccgccgcc
    20821 cgctttgacc ggcatgcgtg acacggcaca aaaaaagccg accggcgaac cggtcggctt
    20881 ttttcagtcg gacgaccgga ttacttggtc agtgccgact tgatctgctc gagggtgctg
    20941 ggatcctcga tggtggtcag atcgcccgga tcgcggcctt ccgccagcgc ctggatggag
    21001 cggcgcagca tcttgccgga gcgggtcttg ggcaggccgg agatgaagtg cacgcgggcc
    21061 gggcgggcaa tggcgccgag gctgccgtcc acgatcttca tcacagcctt ttccagctcc
    21121 acgaccttct cgggcgtatc gacgctggcc ggatccttga ccacggcgaa ggccatgggc
    21181 atctgaccct tgagctgatc cgccacgccc accaccgcca cctcggcaat ggcggaatgg
    21241 gactggatcg cctcctcgat ctcgcgcgtg ccgagacggt gaccagccac gttgatcacg
    21301 tcgtcggtac ggccgaggat cttgtagtaa cccgcgtcgt ccttgatgcc ccagtcgaag
    21361 gacgagtaga ccagcggctc ctggaacagg gagaagtagg tcgacacgaa acgcttatcg
    21421 tcaccccaca ccgtggtgag gcagcccggc ggcagcggcg gcaccacgcc gacgatgccc
    21481 ttctcgttgg ggccgcattc tgagccgtcg tcccggaaca ggcgcacgtc gtagccgtac
    21541 accgggaagc cgggggagcc gagctggacc tcctggcgct cgacaccggg catcagggac
    21601 agcatggccc agccggtttc ggtctgccag tagtggtcga taacctggat ccccagctcg
    21661 tccatcatcc actggtggga ggtctcgtcc atcggctcgc cggcacagta aagggcgcgc
    21721 agcttggaga ggtcgtactt cttgaggaag gccggatcct gcttcttcag cacgcggatg
    21781 gcggtgggtg cgctgaacat cacggtgacg tcctgctcct ggcagatctt ccaccagatg
    21841 ccggcgtcgg gacgcagagg cgtgccttcg tacatgatgg tggccatgcc gttgatcagc
    21901 gggccgtaga tgatgtagct gtgacccacc acccagccga tgtcggaggt ggagaagaag
    21961 gtttcgccgg gattgccgca gtagatgtgc ttcatggacg cggcgagggc gacagcgtag
    22021 ccgccggtgt cacgctgcac gcccttgggt ttgccagtgg tgccggaggt gtacaggatg
    22081 tagctgggct cggaggattc catccaggtc acgggcacct gtgcgtccag gtgcttctcg
    22141 cgcagcgcag cgtagtccac atcgcgaccg gccaccacgg ccatgttggg gtcgaggttg
    22201 cggttgacca tcagcacctt ggcaggcttg tgttcggcca ggttgatggc ttcgtccaac
    22261 aggtgcttgt agggcaccgg acggccgcca cgcatgccgg catcggcgga gacgatcacc
    22321 gtgggcttgg catcgtcgat gcgggtggcc agcgaatggg acgcgaagcc gccgaaaacc
    22381 accgagtgga tggcaccgat gcgggtgcag gccagcatcg cgaagcaggc ttcggcgatc
    22441 atcggcatgt agatcaggac gc
//
LOCUS       DAOQPA010000053        21221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1206_length_21221_cov_18.512993, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000053 DAOQPA010000000
VERSION     DAOQPA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_21221_cov_18.512993"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /gene="flhF"
                     /locus_tag="PKX01_09095"
     CDS             <1..1087
                     /gene="flhF"
                     /locus_tag="PKX01_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HMZ76322.1"
                     /translation="PARKPAEPVRSPLRGDPAAPREAAPRPRATGIEEEARIRALEAA
                     NSQMATELAAIRGMIERQLAGFAWGELNRTAPSRTRLMSDLLEAGFSAALARELADAV
                     PEDASVEDAHRILRTVLSQRLRAMSSDADIIDRGGVYALVGPTGVGKTTTTAKLAARC
                     VVRHGADKLALITTDGYRIGAHEQLRIYGRILGVPVFVVRDATDLRETLAGLRDRHMV
                     LIDTMGMSQRDKMVAEQAAMLTGAGKVKRLLLLNATSRGDTLDDVINAYAGDDLQGCI
                     FTKTDEAMSVAPVLDVAIRHQVEVFYVANGQRVPEDLHLPNRDTLLAQALKELPEPSP
                     FRLAPMEAGLVMAGSPAAAVHAAGGAR"
     gene            1087..1872
                     /locus_tag="PKX01_09100"
     CDS             1087..1872
                     /locus_tag="PKX01_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FleN"
                     /protein_id="HMZ76323.1"
                     /translation="MHAHSDQAAGLRRLFRRTPPAVFALFATGRSPLKTAARAVRSLT
                     EGGRRVVVLEEHGEATGSLFGSFGTDGGADLLATLERGLDPADTLREAADGLWLVRAA
                     ATAQAVPWLDAGHLSRLESAIGELQRRADLLAVHAAGDGAGLTPFAHAAARRLLVVEA
                     SGVGARGACQWIKGLAAAGAGSLEVAVSGARDRADATALFASLSDYTARHVGLPLVWR
                     GELERDGLAEAMSTPLGARHPAEGVQAFVRRLRAWSAQASAAG"
     gene            1963..2727
                     /locus_tag="PKX01_09105"
     CDS             1963..2727
                     /locus_tag="PKX01_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HMZ76324.1"
                     /translation="MYTASGTLDKEQLVIQYAPLVKRIAYHLMAKLPASVQVEDIIQN
                     GMLGLLDAINRYEEGLGAQFETYAVQRIRGAMLDGLRENDWLPRSLRRDMRRIEAAVH
                     QLEQQNGRPPTETELAGSLGMPLADYQHMLQEARGYQLVYFEDFAGDGDEDYLERHIT
                     IEGNDPLTLLMDSNMRDVLIQGIDDLPEREKTVMGLYYEQDMNLREIGEVLGVSESRV
                     CQLHSQAIARLRSRIAGAVNGTAPVKVKGRGRRAAG"
     gene            2756..3502
                     /locus_tag="PKX01_09110"
     CDS             2756..3502
                     /locus_tag="PKX01_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HMZ76325.1"
                     /translation="MDSISLIGITLALAAILVGQVLEGGHIGSLVQPTAFLIVIGGTL
                     GAVMLQSSLRVFRDGLRMLRWVFMPPALDHATLIQKVVSWSVTARKEGLLALEAQIPA
                     LDDPFIAKGLQLLVDGVEPERLREVLEVEIDTWEARMKLGAKIWEAAGGYAPTIGILG
                     AVMGLIHVMENLSDPSKLGSGIAVAFVATIYGVGSANLIFLPMSKKLMGVIATLVTLR
                     EMVVDGLVGIANGDNPRIIESRLSGYAGNA"
     gene            3513..4403
                     /gene="motD"
                     /locus_tag="PKX01_09115"
     CDS             3513..4403
                     /gene="motD"
                     /locus_tag="PKX01_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HMZ76326.1"
                     /translation="MGRRRGRHEEEHENHERWLVSYADFITLLFAFFVVMYSLSSLNE
                     GKYRIMSDSVVNAFRNLAVNSNSDRMIASPIPNLRPPTPQAADAAKTEEARQARAERV
                     RNMAEEIRKVLAPLVADGQVRVTEGAFGITVEINASVLFAPGEAQLGPDAVRALRAVA
                     QVVAAASFPVTVEGHTDNTRIATAMFPSNWELSAVRASSVVRLFVESGVAPDRLTAAG
                     YGDQRPVADNLTPDGRARNRRVTLLIESRVAEPSPDGTVRASGRTDSILPADALPPMP
                     RTQDASAAPAVPASPVPPRP"
     gene            complement(4530..5006)
                     /locus_tag="PKX01_09120"
     CDS             complement(4530..5006)
                     /locus_tag="PKX01_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HMZ76327.1"
                     /translation="MTGHPVLTERLARLVRDEHTQLAGFVELLKQEEALLVENSIDPL
                     LTLAGEKTERYRGLQRLSDERIVLFARMGIPVSGEAIRATLAPDPAALAEWEAVVDLA
                     REAHARNALNGKLITERLQNNQQALSTLLLAAEQPQIYGPDGQARPTGGSRHLGSV"
     gene            complement(5019..5333)
                     /gene="flgM"
                     /locus_tag="PKX01_09125"
     CDS             complement(5019..5333)
                     /gene="flgM"
                     /locus_tag="PKX01_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HMZ76328.1"
                     /translation="MKIDNSVKSAAGLPSGDGRTRNAKESPKTQGSSGGGEKVDISSL
                     SARMQQMEEVISNTPVVDSAKVDEIKQAISDGRFKVDTDKVADGLIESVRQMLSAQTG
                     QA"
     gene            complement(5442..6164)
                     /gene="flgA"
                     /locus_tag="PKX01_09130"
     CDS             complement(5442..6164)
                     /gene="flgA"
                     /locus_tag="PKX01_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HMZ76329.1"
                     /translation="MPNRSPVRPMHRLSIRLLLLLIGLAAAAVASARQDAAPVRAEVA
                     AFLQQQTRSLAGEVRIEVGEFAPDNGLAPCTRLEAFLPPGARAWGRVTAGIRCLAPTP
                     WSAYVPAQVRVTGPYLITTTALAAGQALGPGDVRVEVGEVSAQPADVLTHPTQAAGQI
                     ARLAIAAQRPLVASLLRAPVAVAQGQAVKVITRGPGFQVANDGTALSGGSDGQPVQVR
                     LGNGQVVRGTARVGGYVEIALP"
     gene            6502..6906
                     /gene="flgB"
                     /locus_tag="PKX01_09135"
     CDS             6502..6906
                     /gene="flgB"
                     /locus_tag="PKX01_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HMZ76330.1"
                     /translation="MTDRLDNELRFHQTALNARAYRQQVIASNIANADTPNFKARDLD
                     FREALKGALGGGTASLDLRTTHAAHLPGAVATPMAAALKFRNEEQGAVDGNTVNMDVE
                     RSAFAENAVQYQASVTFINGLLRSMQLAVQGQ"
     gene            6917..7321
                     /gene="flgC"
                     /locus_tag="PKX01_09140"
     CDS             6917..7321
                     /gene="flgC"
                     /locus_tag="PKX01_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HMZ76331.1"
                     /translation="MSMLNVFQVAGSALHAQSTRLNTTASNLANADSVVSSNGQPYRA
                     KQVVFAATPMGNPAAQGVRVTQVVESAAPLRMVYDPKSPAANADGYVAMPNVNVVEEM
                     TNMISASRAYQTNADVMGTAKSLLEKTLTIGQ"
     gene            7341..8015
                     /locus_tag="PKX01_09145"
     CDS             7341..8015
                     /locus_tag="PKX01_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HMZ76332.1"
                     /translation="MSTTVNSTTTSDFVASLQKRASTSSTTTTDAQSKFLTLLTEQLK
                     NQDPLNPMDNAQMTSQLAQISTVDGIEKLNTTLASLLQDSQSNAALQAAALVGQNVMV
                     AGKDLTLSGSKGYGGIELPSAADKVTVTIKDANGLQVRKLELGARDAGVSDFAWDGTT
                     DSGSKAVDGAYTISVTASRGDTALKPVALELTKVGSVLRTASGGVSLDVGNGSLVSLS
                     DIKQIL"
     gene            8029..9597
                     /locus_tag="PKX01_09150"
     CDS             8029..9597
                     /locus_tag="PKX01_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HMZ76333.1"
                     /translation="MAFQQGLSGLSIASKALDAISNNVANSGTVGFKQSAAQFSDVFA
                     ASLASGSSVQVGIGASIDKVSQQFTQGNITTTSNALDLAINGQGFFRMSDNGTISYSR
                     NGQFQTDKNGFIVNSSGLHLTGKVADANGVLTGTTGDIKLNFSDASPSATTAANYNVN
                     LDSRAATPSALAAAVVTGTAVPSLTVGASPANVLTITVDGAPTATSVTIAPSTYADVG
                     SLVSAIQTAVDQTALKGRVKISSDSTGKISVTSARSGRLSDVTVGGAAATALNLPGTT
                     ATSSNSGFSTSDPTSYTSTTSQTVYDSLGNAHTQSLYFVKTAQSNVWDVYTSLDGGFP
                     PEINPATGVHTPTTISFDANGVLQTPTSFATSYTISTGAVVPLDFTVELKGTTQFGNS
                     YGVNQLSQDGYTTGKLSGLTVSADGTIQGNFSNGQSRVMGQVWLASFQNPNGLQSLGG
                     NQWAVTNASGPEQPNAPGTGSLGVVQSAAVEDSNVDLTSELVNMITQQRAYQANAQSI
                     KTQDQVLQTLVNLR"
     gene            9620..10360
                     /gene="flgF"
                     /locus_tag="PKX01_09155"
     CDS             9620..10360
                     /gene="flgF"
                     /locus_tag="PKX01_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HMZ76334.1"
                     /translation="MDRLIYTAMTGAAATLGQQAAVAHNMANATSTGFRAELHRLRAV
                     PVQNAQLPTRAFVVDASVSDDFSTGPLQHTGHTYDMAIQGKGWFAVQMPDGSEAYTRD
                     GSFEVSANGVLQTRSGLPVLGGGGPITIPPDTEVIVGADGTVSTVPSSGARNTTAVVD
                     QLKLVNPPEQDLQRGGDGLFRLAGGTAAPADPAVRVASGYLEGSNVNVVEQMVSMISL
                     SRQFEMQTKMLTTAQENDRSAAQVITKA"
     gene            10509..11291
                     /gene="flgG"
                     /locus_tag="PKX01_09160"
     CDS             10509..11291
                     /gene="flgG"
                     /locus_tag="PKX01_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HMZ76335.1"
                     /translation="MIRSLWIARTGLDAQQTQLDVISNNLANVSTNGYKRARGVFEDL
                     LYQTLRQPGAQSTQQTTIPSGLQIGTGVRPVATERIHTQGNLNQTGNSLDVAIQGSGF
                     FQILLPDGTTAYTRDGAFQKDANGQIVTSSGYPLQPAITIPSNALSLTIGKDGVVTVT
                     LPNQTAPAQIGNIQVATFVNPGGLQSVGENLYLETAASGTPNPNTPGSNGAGVLNQTY
                     VETSNVNVAEELVLMIQTQRAYELNSRAVQTSDTMLGKLTSL"
     gene            11312..11989
                     /locus_tag="PKX01_09165"
     CDS             11312..11989
                     /locus_tag="PKX01_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HMZ76336.1"
                     /translation="MKSPRALTSLVAVALLAGCSALQTTPPTAVHQPMSIRPEPRTAQ
                     ASQPGAIFQQSFSRPLFEDRRARFVGDIITINLVEQTAASKKSSANAERNASIGASIS
                     ALTKVPLAGLNGLDATASDASKFGGKGDAAANNAFTGTITTTVIEVLPNGNLLVSGEK
                     QIAINQGNEFIRFSGVVNPVNVTGSNTVQSTQVADARIEYRANGYIDETERMGWLQRF
                     FMTALPF"
     gene            12043..13113
                     /locus_tag="PKX01_09170"
     CDS             12043..13113
                     /locus_tag="PKX01_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HMZ76337.1"
                     /translation="MALCLVTPAVRAERLKDLASIGGVRNNQLVGYGLVVGLDGSGDQ
                     TTQTPFTVQSIINMLGNLGVTLPPGQSLQLKNVAAVMVTASLPPYARPGQQIDVTVSS
                     MGNARSLKGGTLVMTPLKGADGQIYAMAQGSLAVSGAGGASGGSKVTVNHLAAGRIPG
                     GATVERALPSAVGQGEYVFIELNDADFATAQNAVDAINRLAPGSAQAVDARQIQVRAP
                     DDPNQRVAFLGRVENLDVTPAIQAAKVIVNSRTGSVVMNQKVALQNCAVAHGSLTVTV
                     NNEPQVSQPGALSGGQTVVTNKGSVDIKQEGGTLFNLKAGTNLADVVKALNAVGANPL
                     ELIAILQAMKASGALRAELEVI"
     gene            13115..14065
                     /gene="flgJ"
                     /locus_tag="PKX01_09175"
     CDS             13115..14065
                     /gene="flgJ"
                     /locus_tag="PKX01_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HMZ76338.1"
                     /translation="MAADFSQINALDPRTLGNLQRLAKENSPEAAREAAKQFEGLFLQ
                     QVLKSMRDASPKNGLFDSEESRMYQGMLDQQLALQLASRGGGVGLAKVIERQLGVGRA
                     TADTSGSSPSLDTAIRRSYDAALPAGSAANAAAKSAASAASAPASGLDLPAGAGPRAF
                     VERIWPDAVEASRATGIPARFFVAQAALETGWGKYELKNADGSPSYNLFNIKAGRRWS
                     GDTVSTTTVEYANGTPTRESARFRRYGSYAESFRDYAAMLANNPRYAAVMGQSDASGF
                     ARALQSAGYATDPQYADKLTRIIQGNTLRTALAASASRNA"
     gene            14151..16100
                     /gene="flgK"
                     /locus_tag="PKX01_09180"
     CDS             14151..16100
                     /gene="flgK"
                     /locus_tag="PKX01_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HMZ76339.1"
                     /translation="MGTSLFSIGLTGLNAAQAGLSTTSHNIANVGTAGYSRQSITQST
                     NPAQFTGGGFLGEGTKVDTVKRAYDDFLTRQVLSADTKYNEYDTYNTQIAQLDNLLAD
                     SSAGLTPAYQSFFQGVQEVAANPSSIPARQSLLSAGESLVSRFQTLSDRINEVRSGVE
                     GQIQDTVKSISTYAQQIADLNKRIADAQQAGSSQPANDLLDQRDQAVRELNQLTRVST
                     TVLSDGSMGVFIGTGQPLVMGANATVLQARASQGDPTRFSVNIIAQNGNAITIPESML
                     SGGKLGGLLSLRSETLDSTQNQIGLMAVGLATAFNAQHALGQDLNGNLGTDFFVAPSV
                     SVQPAQGTTTAAPAVTYANIAQLTADDYRLTFTDTTGGYSLTRLSDGTSVSAASVGLS
                     ITPPASSKVGDSFLVQPTRYAAGNMAVALTDTRMIAASAPITTGARSGNLGTGSIGAA
                     VVSSTSSLSSFGTAASPTTLTYSGGNLSGFPAGLPVTYTPPGGSPVTVAAPVASIPYT
                     SGMSIALGGVSFTLSGAIQNNDTFTVAPTVAGVSDSRNAVLLGSLETTKMLLTANGAP
                     SATFQSVYSQMVSAVGNKAREVKVNAEAQNSLLSQATAAQQSITGVNLDEEAANLIRY
                     QQAYQASGKVMSVAAKLFEQVLQLG"
     gene            16118..17308
                     /gene="flgL"
                     /locus_tag="PKX01_09185"
     CDS             16118..17308
                     /gene="flgL"
                     /locus_tag="PKX01_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HMZ76340.1"
                     /translation="MRISTGMIYDAGVASIQQQNSKLLDAQKQVATGRRVLSPADDPV
                     ASARALEVSQSKSVNDLYASNQAYANDALKLVDSKLGSVTDLLTYVRTRAVESGNGTY
                     SSSEFAAIASDMTQQFSALQSLANSQDSTGEYVFAGYRSNAQPFAGSLAGGITYQGDQ
                     GQRSLQISASRILPVTSSGDQIFNQIRAPAGSAYALAGAANTGTALVSATSLSGYNGH
                     TYAISVGAGPAYQVFDSTADPTHASPITPTVTSSGTTTTLAFGGISMTLTGTPAAGDS
                     FSLGTVAGAFDVLGNFVRSLQSGNAAVTAFGANQAIAGMDAAQDSVSQVRSAVGSRMV
                     EVESQQNVTSAMTLQYSETLSNLQDADYASAVSNLTQRSTYLQAAQQSFMKVSNLSLF
                     NYLS"
     gene            17319..17813
                     /locus_tag="PKX01_09190"
     CDS             17319..17813
                     /locus_tag="PKX01_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76341.1"
                     /translation="MIRSLQRVAWGSALLVTLAGCSSVSTEPPHDWLIVPAAGMAAVT
                     NGYLKIPALTAAVAAYAVVDPLAPNWVIREAPLGEDRYRLALRMKGIHTGGDGEARQV
                     FARRAAQLAGQPGFGAYEVVAWQEGLDSTRPFSQRVAEGEIRLLRASRALAAMPPPAT
                     SARP"
     gene            17906..18214
                     /locus_tag="PKX01_09195"
     CDS             17906..18214
                     /locus_tag="PKX01_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ76342.1"
                     /translation="MDHSRAERRRDRRIPLGCKVAIRVEGTREPVVGVCVELSVGGMT
                     IQSSHVPRMDEVFELVVLPPEPGGVHSPLHVRARVRRCDGTAQPGLYEMGVEIIEVLR
                     "
     gene            complement(18226..19002)
                     /locus_tag="PKX01_09200"
     CDS             complement(18226..19002)
                     /locus_tag="PKX01_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76343.1"
                     /translation="MLRRLRIAVLLLILASVALTAWRSQARIEAWRSSLHVTVYPIAA
                     DDAPATRRYIESLTADTFDELEDWFEAEFMRHGKALRRPLAFNLAPPLTALPPPFPAG
                     GSALATARWSLQLRLWAWRHERAPGPAPDVRLFLLFHDPARTPTLAHSVGLEKGRIGL
                     VQLFARRSETPRNAVIIAHELLHTLGAADRYDPVTLHPRWPDGYAEPDRTPRWPQHLA
                     EIMGGRIPVDAGRADIPDGLHEALIGAATAAEIGLSAPRP"
     gene            complement(19084..19668)
                     /locus_tag="PKX01_09205"
     CDS             complement(19084..19668)
                     /locus_tag="PKX01_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76344.1"
                     /translation="MTLRRTPKVLLALLTATLPALSNAGCFPVAGSVRLLPDPTCAVQ
                     SSVSGVVFTGQCFSVNLALIGFPAGTGYAGTTVEPLAGSNGVPTLTPAVIPVDGQPTL
                     PRQMIQTARSVITLGHTERRHSAATTLYTTDVIVFQPKLSSTGDVMPGPTVEQILIAG
                     TDGKGAYAGATGNLTITGNSIGTPAPVVGKICLP"
     gene            complement(19869..>21221)
                     /locus_tag="PKX01_09210"
     CDS             complement(19869..>21221)
                     /locus_tag="PKX01_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HMZ76345.1"
                     /translation="LEAQAIAMAAHNSGGIVIAQVERIAERGTLSSRQVKIPGIFVNC
                     VVVAEKPEYHMQTFIEQYSPGFAGELRVPMSAVPSMEMSERKIIARRAALELRANAVV
                     NLGIGMPEGVASVAAEEKIIDLFTMTAEPGVIGGIPAGGLNFGAAVNTQAIIDQPSQF
                     DFYDGGGLDIAFLGLAQADRVGNLNVSRFGPRLAGAGGFINISQNAKKVVFVGTFTAG
                     NLEIAIQHGKLAILNDGRSKKFVEEVEHRTFSGPEAARRHQPVLYVTERCVFRLDPEG
                     LELIEVAPGIDIERDILARMAFRPIIRGEPALMDERIFGDGPMNLRPDMLEIPMKDRL
                     SYDAENNLFFVNFEGLAVRTEADIERIRATVEHQLAPIGHKVFAIVNYDRFHIEPELT
                     DDYVGMVKGLMDRHYIDVTRYTSSTFLRLKLGEALARSDVVPHMFASADEAQAGLNQR
                     "
BASE COUNT         3549 a         7548 c         6952 g         3172 t
ORIGIN      
        1 acccgcccgc aagcccgccg agccggtacg ctcgcccctg cgcggcgatc cggccgctcc
       61 ccgcgaggcg gcgccccgtc cgcgcgccac cggcatcgaa gaggaagccc gcatccgcgc
      121 cctggaggcc gccaacagcc agatggccac cgaactcgcc gccatccgcg gcatgatcga
      181 gcgccagctg gccggctttg cgtggggcga gctcaatcgc accgcgccgt cccgtacccg
      241 cctgatgagc gacctgctgg aggccggctt ttctgccgcg ctggcgcggg agctggccga
      301 cgccgtgccc gaggacgcgt ccgtcgagga cgcccatcgc atcctgcgca cagtgctgag
      361 ccagcgcctg cgcgccatgt ccagcgacgc cgacatcatc gaccggggcg gcgtgtacgc
      421 cctggtggga cccaccggcg tgggcaagac caccaccact gccaagcttg ccgcccgctg
      481 cgtggtccgc cacggggccg acaagctggc cctcatcacc accgacggct accggatcgg
      541 cgcccacgag caactgcgca tctacggccg gatcctgggc gtaccggtgt tcgtcgtgcg
      601 cgacgccacc gatctgcgcg agaccctggc cggcctgcgc gaccgccaca tggtgctcat
      661 cgacaccatg ggcatgagcc agcgcgacaa gatggtcgcc gagcaggccg ccatgctcac
      721 cggcgcgggc aaggtcaagc gtctgctgct gctcaacgcc accagccgcg gcgacaccct
      781 ggacgacgtc atcaacgctt acgccggcga cgacctgcag ggctgcatct tcaccaagac
      841 cgacgaggcc atgtccgtgg cgccggtgct cgacgtggcg atccgccacc aggtggaggt
      901 cttctatgtg gccaatggtc agcgcgtgcc cgaggatctg cacctgccca accgggacac
      961 cctgctggcc caggccctca aggagctgcc cgagccgtcg ccgtttcgtc tcgcgcccat
     1021 ggaagccggc ctggtgatgg cgggcagccc ggcggccgcc gttcacgctg cgggaggtgc
     1081 ccggtgatgc acgcccattc cgatcaggcc gccggcctgc gccgtctgtt ccgccgcacg
     1141 ccaccggcgg tctttgccct cttcgcgacc gggcggtccc ccctcaagac cgccgcccgc
     1201 gcggtgcgca gcctcaccga aggcggacgc cgggtggtgg tgctggaaga gcacggcgag
     1261 gccaccggct ccctcttcgg cagcttcggc accgacggcg gtgccgacct gctggccact
     1321 ctggagcgcg ggctcgaccc ggccgacacc ctgcgcgagg cggccgacgg cctgtggctg
     1381 gtgcgcgctg ccgccaccgc ccaggcggtg ccctggctgg atgccggtca cctcagccgc
     1441 ctcgaatccg ccattggcga actgcaacgc cgcgccgatc tgctggcggt ccatgccgcc
     1501 ggtgatggtg ccggcctcac accctttgcc catgccgccg cccgccggct gctggtggtg
     1561 gaggccagcg gcgtgggtgc gcgcggtgcc tgccagtgga tcaaggggct tgccgcggcc
     1621 ggagccgggt cgctggaagt cgccgtcagc ggcgcccgcg accgggccga cgccaccgca
     1681 ttgtttgcca gcctgtccga ttacaccgcc cgtcatgtgg ggcttccact cgtgtggcgc
     1741 ggcgagctgg agcgggacgg cctggccgag gccatgagca cacccctcgg cgcgcgtcac
     1801 ccggcagaag gcgtgcaggc cttcgtgcga cgactgcggg cctggtcggc ccaggccagc
     1861 gcggcgggat gaggtcccga atcctgcagg ccccccgatg acgtgtcccc gcccggcggg
     1921 gtccttttcc gccctgaggc gctttcacga agctgacgaa cgatgtacac cgcatccggc
     1981 accctcgaca aagaacagtt ggtgatccag tacgcaccgc tggtcaagcg catcgcctac
     2041 cacctgatgg ccaagctgcc ggccagcgtg caggtggagg acatcatcca gaacggcatg
     2101 ctgggcctgc tcgacgccat caaccgctac gaggaagggc tcggcgccca gtttgagacc
     2161 tacgcggtcc agcgtatccg cggcgccatg ctcgacggcc tgcgcgaaaa tgactggctc
     2221 ccgcgcagcc tgcgccgcga catgcgccgc atcgaggcgg cggtgcatca gctcgaacag
     2281 cagaacggcc gcccgccgac ggagaccgaa ctggccggct ccctcggcat gcccctggcc
     2341 gattaccagc acatgctcca ggaggcccgc ggctatcagc tggtgtattt cgaggacttc
     2401 gccggcgatg gcgacgagga ctacctggag cgtcacatca ccatcgaggg caacgacccg
     2461 ctcaccctgc tgatggacag caacatgcgc gacgtgctga tccagggcat tgacgatctg
     2521 ccggagcggg aaaagaccgt gatgggtctg tattacgagc aggacatgaa cctgcgggaa
     2581 atcggcgagg tgctgggcgt gtccgaatcg cgcgtgtgtc agctgcacag tcaggccatc
     2641 gcgcgcctgc gctcgcgcat cgcgggcgca gtgaatggca ccgcgccggt caaggtcaag
     2701 ggccgcggcc ggcgcgctgc cggctgagcg ctcacccggc agcggaggcg cgtccatgga
     2761 cagcatcagc ctgatcggca tcaccctcgc cctggctgcg atcctggtgg ggcaggtgct
     2821 ggagggcggc cacatcggct ccctggtcca gccgacggcc tttctcatcg ttatcggcgg
     2881 caccctcggt gcggtgatgc tgcagagctc cctgcgggtg ttccgcgacg ggttgcgcat
     2941 gttgcgctgg gtcttcatgc cgccggccct cgatcatgcc accctgatcc agaaggtcgt
     3001 gagctggagc gtcaccgccc gcaaggaagg tctgctggcg ctggaggccc agattccggc
     3061 cctcgacgat cccttcatcg ccaagggcct gcagctgctg gtggacggcg tcgagccgga
     3121 gcgtctgcgg gaagtgctgg aggtggaaat cgacacctgg gaggcgcgca tgaaactggg
     3181 cgccaagatc tgggaggccg ccggcggtta cgcgccgacc atcggcatcc tgggggccgt
     3241 gatgggcctg atccacgtca tggagaatct ctcggacccg tccaagctgg gttccggcat
     3301 cgccgtggcc ttcgtggcca ccatctacgg ggtaggctca gccaacctga tctttctgcc
     3361 catgtccaag aagctgatgg gcgtcatcgc cacccttgtc accctgcgcg agatggtggt
     3421 ggatggtctg gtgggtatcg ccaacggcga caacccgcgc atcatcgagt cacgcctgtc
     3481 cggctacgcc ggcaacgcct gaaggagccg ccatggggcg ccgtcgtggc cggcacgagg
     3541 aggagcacga gaaccacgag cgctggcttg tctcctacgc cgacttcatc accctgctgt
     3601 tcgccttctt cgtggtgatg tactccctgt cgtcgctcaa tgagggcaag taccgcatca
     3661 tgtccgattc ggtggtcaat gccttccgca atctggcggt aaacagtaac agcgaccgga
     3721 tgatcgcctc ccccatcccc aacctgcggc cgccgacacc ccaggcggcc gatgcggcca
     3781 agaccgaaga ggcccgccag gcgcgcgccg agcgggtgcg caacatggcc gaggagatcc
     3841 gcaaggtgct ggcccccctg gtggccgacg ggcaggtgcg ggtcaccgaa ggcgccttcg
     3901 ggatcaccgt ggagatcaac gccagcgtgc tcttcgcgcc cggcgaggcc cagctcgggc
     3961 cggatgcggt acgcgccctg cgcgcggtgg cccaggtggt ggccgcagca agctttccgg
     4021 tgaccgtgga agggcatacc gacaacaccc gcattgccac cgcgatgttt ccgtccaact
     4081 gggagctgtc tgcggtgcgg gcgtcgagcg tggtgcgcct gttcgtcgag agcggcgttg
     4141 cgccggatcg tctgacggcc gccggctatg gcgaccagcg tccggtggca gacaacctca
     4201 cgccggacgg tcgggcacgc aaccggcggg tgaccctcct catcgaatcc cgggtggccg
     4261 agccgtcgcc ggacggcacg gtgcgggcct cggggcggac ggattcgatc ctgccggcgg
     4321 atgcgttgcc gccaatgccg cggacgcagg acgcgtccgc ggcgcccgcc gtgcccgcgt
     4381 caccggtgcc accccgacct tgagacccgg ggccggcggg atggcgctcc gccacgcagg
     4441 cgcatggcga agtgggcatg cgcggatgcg cgtgccggac ggggcgctgt cagtcgggga
     4501 cggtggcggc gggtgtccgc aggggaaggt cagaccgagc cgaggtggcg gctgccgccg
     4561 gtcgggcggg cctgaccgtc cgggccgtag atctggggtt gttcggccgc cagcaggagg
     4621 gtgctcaggg cctgctggtt gttctgcagg cgttcagtga tgagcttgcc attgagcgcg
     4681 ttgcgcgcat gggcctcgcg ggcgaggtca accaccgcct cccactcggc gagggccgcc
     4741 gggtcaggtg ccagcgtcgc ccggatggcc tcaccgctga ccggaatgcc catccgcgca
     4801 aacagcacga ttcgctcgtc cgacaggcgt tgcaggccac gatagcgttc ggtcttttcg
     4861 cccgccaggg tgagcagcgg gtcgatgctg ttttccacga gcagggcttc ctcctgcttg
     4921 aggagttcca cgaagccggc cagctgggtg tgctcgtccc gcaccaggcg ggcgaggcgt
     4981 tcggtgagca caggatggcc ggtcatgggc gtgggggatc aggcctgccc ggtctgggcg
     5041 gagagcatct ggcggacgct ctcgatgagc ccgtcggcca ccttgtcggt gtccaccttg
     5101 aagcgaccgt cgctgatggc ctgcttgatc tcatccacct tggcactgtc cacgaccgga
     5161 gtgttggaaa taacctcctc catctgctgc atgcgtgcag acagggagga gatgtccacc
     5221 ttttcgccac cgccggaact gccctgggtc ttgggcgatt ccttggcgtt gcgcgtacgt
     5281 ccatcccccg acggcaggcc ggcggcggac ttcacggagt tgtcgatctt cacggcatag
     5341 ccctttcttc cacgagtcgg ggaagtcaac gaccccatgg gacaaaactt taacccatcc
     5401 gggatcgggc atgcgtgcgt cgtggcagtc cgctgcgggg ctcagggcag ggcaatctcc
     5461 acatagccgc cgacgcgggc ggtgccacgg acgacctgac cgttgccgag gcgcacctgc
     5521 acgggctggc catcgctgcc gcccgacagg gccgttccgt cattggccac ctgaaagccg
     5581 ggcccgcggg tgatgacctt gaccgcctgc ccctgggcga ccgcgacggg cgcgcgcagc
     5641 aggctggcga ccagggggcg ctgggcggcg atggccaggc gggcgatctg acctgcagcc
     5701 tgggtcggat gggtgagcac gtcggccggc tgggcgctga cttcgccaac ctcgacgcgc
     5761 acgtcccccg gccccagcgc ctgtccggcg gccagtgctg tggtggtgat cagatagggg
     5821 ccggtgaccc gtacctgggc cggcacgtag gctgaccagg gggtcggggc aaggcagcga
     5881 atgccggctg tgacgcgacc ccaggcgcgg gcgcccggcg ggaggaaggc ttcgagccgg
     5941 gtgcagggcg cgagtccgtt gtccggcgca aattcaccca cttcaattcg tacctctccg
     6001 gccaggctgc gggtctgttg ctggaggaag gcagcgacct cggcgcgcac gggcgccgcg
     6061 tcctggcggg cggaggccac agccgcagcc gccagaccga tcagcaggag gagcaggcgg
     6121 atcgacaggc ggtgcatggg gcggacgggc gagcggttcg gcatggtgag tccattggag
     6181 catgccgggg aggtggacgt cgcccggaag tgaccgggct ttcgggggca tttcggggca
     6241 tgtgccgcgc gggggttctg ccgggcggca aactttgccg ccctgccggt caggcctgcc
     6301 gcagggccgc cggtgttcgg gcggccggct gctgcgcccc gcggacgaat tgggccggga
     6361 atgcgctgct gttcagccaa atgtcccgcc aggcgcctct ttagcatggc tggcatggaa
     6421 ggtgcactgg cttcatcggg cgccgccccc gaatgcatgc atgacggcac gcccgcccag
     6481 caacgtgaga ggtgaccgga aatgaccgac cgactggaca acgaactgcg cttccaccag
     6541 accgcgctca atgcgcgcgc ctatcgccag caggtgatcg cgtccaacat cgccaatgcg
     6601 gatacgccca atttcaaagc ccgcgacctg gatttccgcg aggccctgaa gggcgcgctg
     6661 gggggcggca cggcgagcct cgatctgcgc accacccacg cggcgcatct gcccggtgcg
     6721 gtcgccacgc ccatggcggc ggccctcaag ttccgcaacg aggagcaggg cgccgtggat
     6781 ggcaacacgg tcaatatgga tgtggagcgc agcgcctttg ccgagaatgc ggtccagtac
     6841 caggccagcg tcaccttcat caatgggctg ctgcgctcca tgcagctcgc cgtgcagggt
     6901 cagtgaggac tgaatcgtga gcatgctcaa cgtcttccag gtggccggca gtgcgctgca
     6961 tgcccaatcc acccggctca acaccaccgc cagcaacctg gccaacgccg acagcgtcgt
     7021 ctccagcaat ggccagccct accgggccaa gcaggtggtc tttgccgcca cgcccatggg
     7081 caacccggcc gctcagggtg tgcgggtgac ccaggtggtg gagagcgccg cgccgctgcg
     7141 catggtctac gacccgaaga gcccggccgc caacgccgac ggctatgtgg cgatgcccaa
     7201 cgtcaatgtg gtggaggaga tgaccaacat gatctccgcc tcccgcgcct accagaccaa
     7261 cgccgacgtg atgggcaccg ccaagagcct gctggaaaaa accctgacca tcggtcagta
     7321 agccccggaa gatcgccacc atgagcacga ccgtcaattc cacgaccacc tcggacttcg
     7381 tcgccagcct gcagaagcgc gccagcacga gttccaccac caccacggat gcccagagca
     7441 agttcctgac gctgctgacg gagcagctga agaaccagga tccgctcaac cccatggaca
     7501 acgcccagat gacctcccag ctggctcaga tcagcaccgt ggatggcatc gagaagctca
     7561 ataccaccct cgccagcctg ctccaggact cccagtccaa cgcggccctg caggcggccg
     7621 cgctggtggg acagaacgtg atggtggcgg gcaaggatct gaccctgtcc ggcagcaagg
     7681 gctacggtgg catcgagctg ccgtcggcgg cggacaaggt caccgtgacc atcaaggacg
     7741 ccaacggcct gcaggtgcgc aagctggaac tgggtgcccg cgatgccggc gtgtccgatt
     7801 tcgcctggga cggcaccacc gacagcggca gcaaggcggt ggacggcgcc tacaccatca
     7861 gcgtgaccgc ctcccgcggc gacacggcgc tcaagcccgt cgccctggaa ctgaccaagg
     7921 ttggcagcgt gctgcgcacc gccagcggcg gcgtatccct cgacgtcggc aacggctccc
     7981 tggtgagcct gtcggacatc aagcaaattc tctgagcgga gacagaacat ggctttccag
     8041 caaggtttga gcggcctttc catcgcttcc aaggccctcg acgccatcag caacaacgtc
     8101 gccaactccg gcaccgtcgg tttcaagcag tccgcggccc agttctcgga cgtgttcgcc
     8161 gcctctctcg cgtcgggcag ctcggtgcag gtgggcatcg gcgcttccat cgacaaggtg
     8221 tcccagcagt tcacccaggg caacatcacc accaccagca atgcgctgga cctggccatc
     8281 aacggccagg gttttttccg gatgagcgac aacggcacca tcagctacag ccgcaacggg
     8341 cagttccaga cggacaagaa cggcttcatc gtcaattcgt cgggcctgca tctgaccggc
     8401 aaggtggccg acgccaacgg cgtgctcacc ggcaccaccg gcgacatcaa gctcaatttc
     8461 agcgacgcca gcccgtcggc caccaccgcc gccaactaca acgtcaacct ggattcccgc
     8521 gccgcgacgc cctccgcgct ggccgccgcg gtggtcaccg gcacggcggt cccgtccctc
     8581 accgtggggg cctcgccggc caatgtgctg accatcaccg ttgacggtgc cccgacggcc
     8641 acgtcggtca ccatcgcccc gagcacctac gcggacgtgg gctccctcgt ttccgccatc
     8701 cagaccgccg ttgatcagac cgcgctgaag ggccgggtca agatctccag cgacagcacc
     8761 ggcaagatca gcgtcacctc ggcgcgcagc gggcgcctgt ccgacgtgac ggtcggcggt
     8821 gcggcggcca ccgcactcaa tctgccgggc accacggcca cctccagcaa ttcgggattc
     8881 agcacctccg accccaccag ctacaccagc acgacctccc agacggtgta cgactccctg
     8941 ggcaacgccc acacccagtc cctgtatttc gtgaagaccg cccagtccaa cgtgtgggac
     9001 gtctacacca gcctggacgg cggtttcccg cccgagatca acccggccac cggcgtccat
     9061 acgcccacca ccatcagctt cgacgccaac ggcgtgttgc agacgcccac ctccttcgcg
     9121 accagctaca ccatctccac cggcgccgtc gtgcccctgg atttcacggt ggagctgaag
     9181 ggcaccaccc agttcggcaa ctcctacggc gtcaatcagc tcagccagga cggctacacc
     9241 accggcaagc tgagcggcct cacggtcagc gccgacggca ccatccaggg caacttcagc
     9301 aacggccagt cgcgggtgat gggccaggtg tggctggcgt ccttccagaa ccccaacgga
     9361 ctccagtccc tcggcggcaa ccagtgggcc gtgaccaatg cctccggccc ggaacagccc
     9421 aacgcgccgg gcaccggcag cctgggtgtc gtccagtccg ccgcggtgga ggactccaac
     9481 gtggatctca cctccgaact ggtgaacatg atcacccagc agcgcgccta ccaggccaac
     9541 gcccagtcca tcaagaccca ggaccaggtg ctgcagaccc tggtgaacct gcgctgagcc
     9601 ggcctgacgg gactcgacca tggaccgcct gatctacacc gccatgaccg gcgccgccgc
     9661 caccctcggc cagcaggccg cggtggccca caacatggcc aacgccacca gcaccggctt
     9721 cagggccgag ctgcatcgcc tgcgcgcagt gccggtccag aacgcccaac tgccgacccg
     9781 cgccttcgtg gtggacgcca gcgtttcgga cgacttcagc accgggccgc tccagcacac
     9841 cggccacacc tacgacatgg ccatccaggg caagggctgg ttcgccgtgc agatgcccga
     9901 tggcagcgag gcctataccc gcgacggcag cttcgaggtc agcgccaacg gcgtgctcca
     9961 gacccgcagc ggcctgcctg tgctgggcgg cggcgggccc atcaccattc cgccggacac
    10021 cgaggtcatc gtcggcgccg acggcaccgt ctccacggtg cccagcagcg gcgcccgcaa
    10081 caccaccgcg gttgtggacc agctcaagct cgtcaacccg cccgagcagg atctgcagcg
    10141 gggcggcgac ggcctcttcc ggcttgccgg cggcactgcc gccccggcag atcctgccgt
    10201 gcgggtcgct tccggctacc tggaaggcag caacgtcaac gtggtcgagc agatggtcag
    10261 catgatctcc ctgtcccgcc agttcgagat gcagaccaag atgctcacca ccgcccagga
    10321 aaacgaccgc tccgccgccc aggtcatcac caaggcctga tccggcaccc ggcggccgat
    10381 ccgggcgtcc gaattgacgg cctttcccgg tcctgctccg cgcctggaac ggagcttgca
    10441 cggactagca tggaacccgt cgcaaccttt gccgcccccg gggcggcccc gactctcagg
    10501 agcccgccat gatccgctcc ctctggatcg cccgcaccgg cctcgacgcc cagcagaccc
    10561 agctcgacgt catctccaac aatctggcca acgtctccac caacggctac aagcgcgccc
    10621 gtggcgtctt cgaggatctc ctctaccaga ccctgcgcca gccgggcgcc cagtccaccc
    10681 agcagaccac cattccctcc ggcctgcaga tcggcaccgg cgtgcgtccc gtcgccaccg
    10741 agcgcatcca cacccagggc aacctgaacc agaccggcaa cagtctcgat gtggccatcc
    10801 aggggtccgg cttcttccag atcctgctgc ccgacgggac caccgcctac acccgcgacg
    10861 gcgccttcca gaaggacgcc aacggccaga tcgtcacttc cagcggctac ccgctgcagc
    10921 cggccatcac catcccgtcc aacgcgctca gcctcaccat cggcaaggac ggcgtcgtca
    10981 cggtgaccct gcccaaccag accgcgccgg cccagatcgg caacatccag gtcgccacct
    11041 tcgtcaatcc cggcggcctg cagagcgtgg gcgagaacct ctacctggaa accgccgcca
    11101 gcggcacacc caaccccaac acgccgggca gcaacggcgc cggcgtgctc aaccagacct
    11161 acgtggaaac ctccaacgtc aatgtggccg aggaactggt gctgatgatc cagacccaac
    11221 gcgcctacga actcaactcc cgcgccgtgc agacctccga caccatgctc ggcaagctca
    11281 cgtccctgta agtcccggcc ggagacgcac catgaaatcc ccccgtgccc tgacgtccct
    11341 cgtcgccgtc gcgctgctgg ccggctgctc cgccctgcag accacgcccc ccaccgccgt
    11401 gcatcaaccc atgagcatcc gccccgagcc gcgcacggcg caggcttccc agcccggcgc
    11461 gatcttccag caatccttct cgcggcccct gttcgaagac cggcgtgccc gcttcgtggg
    11521 cgacatcatc accatcaacc tcgtggagca gaccgccgcg agcaagaagt cctcggccaa
    11581 cgccgaacgc aacgcctcca tcggagcctc catctcggcc ctgaccaagg tgcccctggc
    11641 cgggctcaac gggctcgacg ccaccgcctc tgacgccagc aagttcggtg gcaagggcga
    11701 cgcggcggcc aacaacgcct tcaccggcac catcaccacc accgtgatcg aagtcctgcc
    11761 caacggcaac ctgttggtgt ccggtgaaaa gcagatcgcc atcaaccagg gcaacgagtt
    11821 catccgcttc tccggcgtgg tcaatccggt caatgtcacc ggttccaaca ccgtccagtc
    11881 cacccaggtg gccgacgccc gcatcgaata ccgggccaac ggctacatcg acgagactga
    11941 gcgcatgggc tggctgcaac gcttcttcat gaccgcgctg ccgttctgag gatcgccatc
    12001 gtgaaacccc tgtccgtccg cacgctctgt ttcgcccttg ccgtggccct gtgcctcgtc
    12061 acaccggctg tccgcgccga gcgcctgaaa gacctcgcca gcatcggcgg ggtgcgcaac
    12121 aaccagctgg tgggctacgg cctggtggtg ggcctcgacg gctccggcga ccagaccacc
    12181 cagacgccct tcaccgtgca gagcatcatc aacatgctcg gcaacctggg cgtcaccctg
    12241 ccgccgggcc agtccctgca gctcaaaaac gtggccgccg tgatggtcac cgccagcctg
    12301 ccgccctatg cccggccggg ccagcagatt gacgtgaccg tctcgtccat gggcaacgcc
    12361 cgttccctca agggcggcac cctggtcatg acgcccctca agggcgccga cgggcagatc
    12421 tacgcgatgg cccagggcag tctggcggtg agcggcgccg gcggggcgtc cggcggcagc
    12481 aaggtcaccg tcaaccacct ggccgccggc cgcattcccg gcggcgccac cgtggagcgg
    12541 gccctgccca gcgcggtggg gcagggcgag tacgtgttca tcgagctcaa cgacgccgat
    12601 ttcgccaccg cccagaacgc cgtggacgcc atcaaccgcc tcgccccggg cagcgcccag
    12661 gcggtggatg cgcgccagat tcaggtgcgc gcgccggacg atcccaacca gcgcgtggcc
    12721 ttcctcggcc gcgtcgagaa tctggacgtg acgccggcca tccaggccgc caaggtcatc
    12781 gtcaattccc gcaccggctc ggtggtgatg aaccagaagg tggccctgca gaactgcgcg
    12841 gtggcccacg gcagcctcac cgtcacggtg aacaacgagc cccaggtgag ccagccgggc
    12901 gccctttcag gcggccagac cgtggtcacc aacaagggct cggtggacat caagcaggaa
    12961 ggcggcaccc tcttcaacct gaaggccggc accaacctgg ccgacgtggt caaggccctc
    13021 aacgcggtcg gcgccaatcc cctcgaactc atcgccatcc ttcaggcgat gaaggccagc
    13081 ggcgccctgc gggccgaact ggaagtgatc tgacatggcc gccgacttca gccagatcaa
    13141 cgccctcgac ccgcgcaccc tgggcaatct ccagcgtctc gccaaggaga attcccccga
    13201 ggcggccagg gaagcggcca agcagttcga aggcctgttc ctgcaacagg tgctcaagtc
    13261 catgcgcgat gcctcgccaa agaacgggct cttcgacagc gaagaatcgc gcatgtacca
    13321 gggcatgctc gaccagcagc tggccttgca actcgccagc cggggcggcg gcgtgggtct
    13381 ggccaaggtc atcgaacgcc agctcggcgt cgggcgcgca accgccgaca cgtcgggcag
    13441 ttcgccgtcg ctggacaccg ccatccggcg cagctatgac gccgccctcc ccgccggcag
    13501 tgcggcaaac gcggcggcaa aatctgccgc atcggcagcg tctgccccgg caagcgggct
    13561 cgatctgccg gccggtgccg ggccacgggc cttcgtcgaa cggatctggc cggacgccgt
    13621 cgaggccagc cgtgcgaccg gcatcccggc ccgcttcttc gttgcccagg cggcgctgga
    13681 aaccggctgg ggcaagtacg aactgaagaa cgccgacggc agcccgagct acaacctctt
    13741 caacatcaag gccggccggc ggtggtccgg cgacacggtg agcaccacca ccgtggagta
    13801 cgccaatggc acgccgaccc gcgaatccgc gcgcttccgg cgttatggct cctatgccga
    13861 atccttccgc gactacgcgg cgatgctcgc caacaacccg cgctacgcgg cggtgatggg
    13921 ccagagcgac gccagcggct tcgcccgcgc gctgcaatcg gccggctacg ccaccgatcc
    13981 ccagtacgcg gacaagctga cccgcatcat ccagggcaac accctgcgca ccgctctcgc
    14041 ggcgagcgcc tcccgcaacg cctgagccca cgttccgtgc cgcttggcgc gaggctggca
    14101 gagatcttgc tgttccctcc gcagacactt ccgacagaag gacgcggatc atgggcacca
    14161 gcctgttcag catcggactc accggcctca acgccgccca ggcgggcctg tccaccacca
    14221 gccacaacat cgccaacgtg ggcacggccg gctacagccg ccagtccatc acccagagca
    14281 ccaacccggc ccagttcacc ggcggcggct ttctgggaga aggcaccaag gtcgatacgg
    14341 tcaagcgcgc ctacgacgac ttcctgaccc gtcaggtgct gagcgccgac accaagtaca
    14401 acgagtacga cacctacaac acccagatcg cccagctcga caatctgctg gcggattcca
    14461 gcgccggtct cacgccggcc taccagtcct tcttccaggg cgtgcaggaa gtggccgcca
    14521 atccgtccag cattcccgcg cggcaatccc tgctgtcggc cggcgagagt ctggtgtcgc
    14581 gcttccagac cctgtccgac cgcatcaacg aagtccgctc cggcgtggag ggccagatcc
    14641 aggatacggt caagtccatc agcacctatg cccagcagat cgccgacctg aacaagcgca
    14701 tcgccgacgc ccagcaggct ggctccagtc agcccgccaa cgatctgctg gaccagcgcg
    14761 accaggcggt gcgcgagctc aaccagctca cccgcgtgtc caccaccgtc ctgagcgacg
    14821 gctccatggg ggtcttcatc ggtaccgggc aaccgctggt gatgggcgcc aacgccaccg
    14881 tgcttcaggc ccgggcctcc cagggggatc cgacgcgctt cagcgtcaac atcatcgccc
    14941 agaacggcaa cgccatcaca attcccgaga gcatgctgag cggcggcaag ctgggcggac
    15001 tcctgtcctt gcgcagcgag actctggaca gcacccagaa ccagatcggc ctgatggccg
    15061 tcgggctggc caccgccttc aacgcccagc acgcgctcgg gcaggatctg aacggtaatc
    15121 tcggaacgga cttcttcgtc gcgcccagcg tgtcggtcca gcctgcccag ggcacgacca
    15181 cggcggcgcc agccgtgact tacgccaaca tcgcccagct caccgccgac gactacaggc
    15241 tcaccttcac cgacaccacg ggcggttact ccctgacccg tctgtccgac ggcacctcgg
    15301 tcagcgccgc ctccgtcggc ctcagcatca ccccgccggc cagctccaag gtgggggaca
    15361 gcttcctcgt ccagcccacc cgctacgcgg cgggcaacat ggccgtggcc ctcaccgaca
    15421 cgcgcatgat cgccgcgtcc gcgcccatca ccaccggcgc ccgtagcggc aacctgggca
    15481 ccggcagcat cggtgcagcc gtggtgagca gtacgagcag cctgtccagc ttcggaacgg
    15541 cagcctcgcc gacgaccctg acctattccg gcggcaacct cagcggcttt cccgccggcc
    15601 tgccggtcac ctacacgccc cctggcggct cgcccgtcac cgtggcggcg ccggtggcgt
    15661 ccattcccta caccagcggc atgagcatcg cccttggtgg cgtgtccttc accctgtccg
    15721 gcgcgatcca gaacaacgac accttcacgg ttgcccccac cgtggcgggc gtgtccgaca
    15781 gccgcaacgc ggtgctgctc ggctcgctgg aaaccaccaa gatgctgctg acggccaacg
    15841 gggccccgtc ggccaccttc cagtccgtgt attcccagat ggtcagtgcc gtcggcaaca
    15901 aggcgcggga ggtgaaggtc aatgccgagg cccagaattc cctgctgtcc caggccactg
    15961 ccgcccagca gtccatcacc ggcgtgaatc tcgatgaaga agcggccaat ctgatccgtt
    16021 atcagcaggc ataccaggcc agcggcaagg tcatgagcgt ggcggccaag ctcttcgagc
    16081 aagtgctgca gctcggctag gaaccaggag aaccctcatg cgcatcagta ccggcatgat
    16141 ctacgacgcg ggcgtcgcgt cgatccagca acagaacagc aagcttctcg atgcccagaa
    16201 gcaggtggcg accggccggc gcgtgctctc gccggcggac gatccggtgg cctcggcgcg
    16261 ggcgctggaa gtctcccagt cgaagagcgt gaatgatctc tacgcgtcca accaggccta
    16321 cgccaacgac gccctcaagc tggtcgacag caagctgggt tcggtgaccg atctgctcac
    16381 ctacgtacgc acccgcgccg tcgaatccgg caacgggacc tacagcagct cggagtttgc
    16441 cgccatcgcg tccgacatga cccagcagtt ctcggcgctc cagtccctcg ccaacagcca
    16501 ggactccacc ggcgagtacg tcttcgccgg ctatcgcagc aatgcccaac cctttgccgg
    16561 cagcctggcc ggcggcatca cttaccaggg cgaccagggc cagcgcagcc tgcagatctc
    16621 ggcctcgcgc atcctgccgg tgaccagctc cggtgatcag atcttcaacc agatccgcgc
    16681 gccggccggc tccgcctatg ccctcgccgg tgccgccaac accgggaccg ccttggtgtc
    16741 ggccacctcc ctgagcggct acaacggcca cacctacgcc atcagcgtcg gtgccggacc
    16801 ggcctatcag gtcttcgact ccacggccga cccgacccac gcgtcaccga tcaccccgac
    16861 cgtcacctcc agcggcacca ccaccacctt ggcctttggc ggcatcagca tgacgctcac
    16921 gggcaccccg gcggccggcg acagcttttc cctgggtacc gtggcgggcg ccttcgacgt
    16981 gctgggcaac ttcgtcagat cgctccagtc cggtaacgcg gcggtgaccg cgttcggcgc
    17041 caaccaggcc atcgcaggca tggacgcggc ccaggacagc gtgtcccagg tgcgctccgc
    17101 cgtcggttcg cgcatggtgg aagtggagag ccagcagaac gtcaccagcg ccatgaccct
    17161 gcaatactcc gagaccttgt cgaacctgca ggacgcggac tatgcgtcgg cggtgagcaa
    17221 cctgacccag cgctccacct acctgcaggc cgcccagcag tccttcatga aggtgagcaa
    17281 tctgtccctc ttcaactacc tcagctgaga ggccggtcat gattcggtcc ctgcaacgcg
    17341 tggcctgggg tagcgcgctc ctcgtcacgc tggccggctg cagcagtgtc agcaccgagc
    17401 cgccccacga ctggctcatc gtgccggccg ccggcatggc ggccgtcacc aacggctacc
    17461 tgaagatccc ggcactgacc gccgccgtgg cggcctatgc ggtggtcgat ccgctggcgc
    17521 ccaactgggt gatccgcgag gcgcccctgg gcgaggaccg ctaccggctg gccctgcgca
    17581 tgaagggcat ccacaccggc ggcgacgggg aggcccgtca ggtcttcgcc cgccgcgcgg
    17641 cgcagctggc gggccagccc ggcttcgggg cctacgaagt ggtggcctgg caggagggcc
    17701 tggacagtac gcgccccttc tcccagcgcg tggccgaagg cgagatccgc ctgctccgcg
    17761 cctcgcgggc gcttgcagca atgcctccgc cggccacgtc ggcccggccc tgagcggtcg
    17821 tccgcctgca agcgtctctg aaccttgcgg ggccgcggcg cattccgtaa ggtgcgcgct
    17881 tcacgccaag ggaaactcta cgaacatgga tcacagcagg gccgaacgac ggcgcgaccg
    17941 gcgcattcct ctgggatgca aggtcgccat ccgggtcgag ggcacacggg agccggtggt
    18001 gggcgtctgc gtcgagctga gcgtgggcgg catgaccatc cagtccagcc acgtgccgcg
    18061 catggacgag gtgttcgagc tcgtcgtcct gccgcccgaa ccgggtggcg tccattcccc
    18121 gctccatgtg cgcgcccggg tgcggcgctg tgacggcacg gctcagcccg gtctctatga
    18181 gatgggtgtc gagatcatcg aagtgctgcg ttaggccccg cggcgtcacg ggcggggtgc
    18241 gctcaggccg atctccgcgg ccgtggcggc gccgatcagg gcctcgtgca ggccgtccgg
    18301 gatgtccgcg cgcccggcgt ccaccggaat ccgtccgccc atgatctctg ccaggtgctg
    18361 cggccagcgc ggcgtgcggt cgggttcggc gtagccgtca ggccagcggg ggtgcagcgt
    18421 gacggggtcg tagcgatcag cggcgcccag ggtgtggagc agctcgtggg caatgatcac
    18481 cgcattgcgc ggggtctcgc tgcgccgggc aaagagctgc acgaggccga tgcggccctt
    18541 ttccagtccg accgaatgcg ccagcgtcgg cgtgcgcgcc ggatcatgaa agagcaggaa
    18601 caggcgcacg tccggcgcgg ggcccggggc ccgctcatgg cgccaggccc acaggcgcag
    18661 ctgcaggctc cagcgcgcgg tggcaagggc gctgcccccg gccgggaagg ggggcggcag
    18721 cgcggtcagg ggcggggcga ggttgaaggc aaggggacgt cgcagggctt tgccatggcg
    18781 catgaactcc gcttcgaacc agtcttcgag ttcatcgaag gtgtcggccg tgaggctttc
    18841 gatgtaacgc cgggttgcgg gggcgtcgtc cgcggcgatc ggatagaccg tgacgtgcag
    18901 cgaactgcgc caagcctcga tgcgggcctg actgcgccag gcggtcaggg cgacgctggc
    18961 gaggatcagc agcaggacgg cgatacgcag gcggcggagc acggaggacg ggcgagcatg
    19021 acgacggggc gatggccatc gtcgtccgga gaagggtccg gcgaggccgg acccgtgagc
    19081 ggatcagggc agacagatct tgccgacgac cggtgcgggc gtgccgatgc tgttgccggt
    19141 gatggtgagg ttgccggtgg cgccggcgta ggcacccttg ccgtcggtgc cggcgatcag
    19201 gatctgctcc accgtgggcc ccggcatcac gtcgccggtg ctgctgagct tgggctggaa
    19261 gacgatcacg tcggtggtgt agagcgtggt ggccgcgctg tggcgacgct cggtgtggcc
    19321 gagggtgatc accgagcggg cggtctggat catctgacgg ggcagggtgg gctggccgtc
    19381 caccgggatc accgccgggg tcagggtcgg cacgccgttg gagcctgcca ggggttccac
    19441 ggtggtgccg gcatagccgg tgccggccgg aaatccgatc agggcgagat tgacgctgaa
    19501 gcactggccg gtgaacacga cgccgctgac ggacgactgg accgcgcagg tcgggtcggg
    19561 cagcagccga acgctgccgg caacagggaa gcagccggcg ttggacaggg ccggcagggt
    19621 cgccgtgagc agggcgagca ggactttcgg ggtgcggcgg agggtcatga cggttcctcg
    19681 ggtggcaggg tggacagggt tgtcggcaag ggtgtgaccg ggtgggcggg cgcccgtaga
    19741 cgccatggag cgcactatag cgactcgctg aaattgcgtg tgggttttgc aggatcttgc
    19801 agttctgccg atttgaaggc gaaaaaaacc cggctcggag ccgggttcct cgtgggggcg
    19861 ggggcttgtc agcgctggtt gaggcccgcc tgggcctcgt cggcgctggc gaacatgtgg
    19921 ggcaccacgt cgctgcgggc gagggcctcg ccgagcttga gacgcaggaa ggtgctggag
    19981 gtgtagcgcg tcacgtcgat gtaatggcgg tccatcagac ccttgaccat gcccacgtag
    20041 tcgtcggtga gttccggttc gatgtggaag cggtcgtaat tgacgatggc gaacaccttg
    20101 tggccgatgg gggcgagctg gtgctccacc gttgcgcgga tgcgctcgat gtctgcttcg
    20161 gtgcgtaccg ccaggccctc gaaattgacg aagaacaggt tgttctcggc gtcgtagctg
    20221 aggcgatcct tcatggggat ctccagcatg tcggggcgca ggttcatggg gccatcgcca
    20281 aagatgcgtt cgtccatcag ggctggttcg cccctgatga tggggcggaa ggccatgcgg
    20341 gcgaggatgt cccgttcgat gtcgatgccc ggcgccacct cgatcagctc caggccctcc
    20401 gggtcgagac ggaacacgca gcgctcggtg acgtagagca ccggctggtg gcgccgcgcg
    20461 gcttccgggc cgctgaaggt gcggtgctcc acctcctcga caaatttttt ggagcgaccg
    20521 tcgttgagga tggcgagctt gccgtgctgg atggcgatct ccaggttgcc ggcggtgaag
    20581 gtgccgacga agaccacctt cttggcgttc tggctgatgt tgatgaagcc gccggcgccg
    20641 gccaggcgcg ggccgaagcg ggagacgttg aggttgccga cgcggtcggc ctgggcgagg
    20701 ccgaggaagg cgatgtccag cccgccgccg tcgtagaagt cgaactggct gggctggtcg
    20761 atgatggcct gggtgttcac cgcggcgccg aagttgagcc cgccggcggg aatgccgccg
    20821 atcacgcccg gctcggcggt catcgtgaag agatcgatga tcttctcttc ggcagccacc
    20881 gaggcgacgc cctcgggcat gccgatgccc aggttcacca ccgcgttggc gcgcaactcc
    20941 agcgcggcgc ggcgggcaat gatcttgcgt tcgctcatct ccatggacgg aacggcggac
    21001 atgggcaccc gcagctcgcc ggcaaagccc gggctgtact gctcgatgaa ggtctgcatg
    21061 tggtattcgg gcttttcggc caccaccacg cagttcacga agatgccggg gatcttcacc
    21121 tggcgcgaac tcagggtgcc ccgctcggcg atacgctcca cctgggcgat gacgatgccg
    21181 ccggagttgt gggccgccat ggcgatggcc tgggcctcca g
//
LOCUS       DAOQPA010000054        21079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1219_length_21079_cov_19.175371, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000054 DAOQPA010000000
VERSION     DAOQPA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21079
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_21079_cov_19.175371"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKX01_09215"
     CDS             <1..263
                     /locus_tag="PKX01_09215"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HMZ76346.1"
                     /translation="YGVRKINIDTDIRLAMTGAIRKFFVENPSKFDPREFNKPAREAA
                     KLVCKARFEAFGAAGNASKIKPVSLDKIAARYKSGELNQIVR"
     gene            342..716
                     /locus_tag="PKX01_09220"
     CDS             342..716
                     /locus_tag="PKX01_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76347.1"
                     /translation="MAVPWSTLFGAIPWSDVIARAPDIAQGARKLWQKAGRRGDPPPD
                     ASPVAPSLDVRVDALAARLGDIAARQQDSAELLAALATQQAELIRATDALHRRARLLG
                     IACAILGLALAGLATAILVRAA"
     gene            842..2143
                     /locus_tag="PKX01_09225"
     CDS             842..2143
                     /locus_tag="PKX01_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HMZ76348.1"
                     /translation="MTPTTLFAEHDLRQTLNDEFHARPPVPLESPQLISYLAFHQQDV
                     AAGEAQRHLAELGERLGRPLDAGGPHCLLDFGAFQLKWELHNEFTSYTFIRPVARGEG
                     LVGSTSALAVVPQDWLRAIPGRLMVATHVELRSAAEVSPEEVMAQLSPTGRQMVGAMV
                     ADGAAWVFTDFLFEDGFSRFLVIDVSLTRRQAGRTVQRLVEIETYRLMALLAFPVAKD
                     VGRLLGRAESDLARLMDRTGSARSPEDERGVLADLSALAAEVEHSVARTTFRFGAARA
                     YHSLVMQRIEELRERRITGLPTFHEFMERRLLPAMNTCQTMARRQEDLSGRVARNSQL
                     LRTRVEVELERQNQELLAQMNRRAKLQLHLQETVEGLSVVAITYYASQLVQYMAKGVK
                     HFIEPLTPEGLTAAAIPVIAGIVALGLHRMRKALAAADGGH"
     gene            2227..3147
                     /locus_tag="PKX01_09230"
     CDS             2227..3147
                     /locus_tag="PKX01_09230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMZ76349.1"
                     /translation="MTTPLFESSLRSLPLLGRGKVRDIYAVDADRLLIVTSDRLSAFD
                     VILPDPIPRKGEVLTAMANFWFARLGHIIPNQLTGVDPESVVAADERDQVRGRALVVK
                     RLRPLPIEAVVRGYVIGSGWKDYQDTGAICGIALPAGLKQAQKLPAPIFTPASKADVG
                     DHDENISFEQAQARTAAQFADSGLDGARLVSEARDAALALYSEASAYAAGRGILIADT
                     KFEFGVDAAGTLHLIDEALTPDSSRFWPADSYAEGISPPSFDKQYVRDYLETLDWGKK
                     APGPTLPADVIARTSAKYVEAYERLTGLSL"
     gene            complement(3190..4059)
                     /locus_tag="PKX01_09235"
     CDS             complement(3190..4059)
                     /locus_tag="PKX01_09235"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ76350.1"
                     /translation="MDESELSLVEQAFLHTITMELERGELKFPTAMEVTLRVMEVLDD
                     PDADIARIARMVSAEPLLAARTVALANSAWYNPGGRLVADVPRALARIGIAKLRALAL
                     TVAAEQLATKDMLGPFQPQMQVLWAHCVDVAATACVLARHCSRINPDEAFFAGMVHDI
                     GQFFLLARVWEYPELLREGSPLSDLVRVWHPPIGRAVLASLHMPQDLVDAVDDPELYG
                     GRWPPENVADLIFIANLLAETGNPFSLEPEPFRNGLAKVATLGLDDVEIGAVLKESAE
                     EREALIACFSIHL"
     gene            4147..6216
                     /locus_tag="PKX01_09240"
     CDS             4147..6216
                     /locus_tag="PKX01_09240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HMZ76351.1"
                     /translation="MTTATAPSPLLQPTGLPRFDAITPDQVEPGLRSLLDAYGATLAR
                     LQDDPTTPTWANFVEPIELEGERLGRAWGVVVHMHSVMDTPEWREAYNAMLPEVTRFY
                     AELGQNLKLFEKYKAIAEGPEFAGLTPARQRILDNELRGFRLSGAELPDARKPRFKEI
                     QEELAGLSARFSENLLDATNAFSHLVTDPARLAGLPDDVVTAAREAAAKAGQEGWKFT
                     LKMPSYLPVMQYADDRALRETMYRANGTRAAEIDNGFSKPEWNNGPLIERILALRAEE
                     AAMLGYRTYADVSLVPKMADSPEQVLGFLRELAAKARPFAERDLAELATFARDELGLD
                     PLEPWDTGYASEKLRVARYDFSEQEVKQYLPEPKVLQGLFDVVEKLYGVRILPDSGPV
                     WHPDVRFFRIERQGALVGQFYLDLYARETKKGGAWMDSAISRRRLGDGIETPVAYLVC
                     NFPGPVGGKAATFSHDDVITLFHEAGHGLHHLLTRVDDLAVSGIHGVEWDAVELPSQF
                     MENFCWEWDVVREMTAHADTGEALPRALFDKMLAAKNFQSGMQTVRQLEFALFDLRLH
                     LDFNPANPRVMDLLDEVRQEVAVLVPPAWHRFPNSFSHIFAGGYAAGYYSYKWAEVLS
                     ADAFAAFEEAGRPQGSVLDAVTGARFWEEILAVGGSRPALESFKAFRGREPQVDALLR
                     HSGMVPA"
     gene            6213..6680
                     /locus_tag="PKX01_09245"
     CDS             6213..6680
                     /locus_tag="PKX01_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HMZ76352.1"
                     /translation="MKRRSALLLLIAAAPALAQTAYRWVDDKGRVQYSDQPPPAAVPQ
                     YDQRNISANQSGSLQQSFATRQAAGKYPVTLYVSKDCGKACEDGRAFLKKRTIPFSEQ
                     RIESAEDVATYKARIGAEPFVPTLLVGREKETGFAAPAWDNLLDKAGYPAARR"
     gene            6865..8952
                     /locus_tag="PKX01_09250"
     CDS             6865..8952
                     /locus_tag="PKX01_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76353.1"
                     /translation="MKTRNRLIAEALGGALFLSACGGGGGGSSTPASPQTPASVDVTV
                     TPSLGKFSTGCSVELRTGSGTLLGSAPITDAGNAFIRVTGYSGPVIAQVKGASGCTYF
                     DEATATQVPFGADRSLSAVSDTLRVDQGINVLTHLAASRVLDGDKLATGKTADTVKLE
                     NLTIQRMFQVADMFAAPTLLGNASDRLDNTAAGQLAVQLAALSELAHTRATDVATLAA
                     TLATDLADGTLDTFGASEYQVAAQTTVAKLASPSAQASLLILSQTPSLIVSTRDVQSD
                     VQKVIAAGPALAQAKQLFADLRTSVLALSNSAGTGTLDAENALLKADFQHGLGVDMLI
                     DGVKFMLESASQFFDSGTSRPGSTHSGWVGTCYQDSTTQATCHVSGVDDGSVYTSYQV
                     RLTKTGAGTASWAFTQVYRYQNGQSTQTAAPSGMSGSFTYSANAATAKGQFIPMTGDA
                     ERSAIDISLGFTTTGTTGAIRFSATGSANSVKTDGTSTLQVDLTKLDLDPVSQVNQMV
                     LSLTGPRHRFDGALTLSGQVASADGSIAQPKSLKFVGTFTDTASHYTVLDGTVTASQD
                     WTGYDPRLANSDGNFARYAFSFDGVANKGPGAAGITLSFSGDNQAGPHDQKIALKFTS
                     ASGIVVTGTGVQTPARPDDLWNWTFANENGIKATYAQSSRSGKVTAGDGSDLGTLSSQ
                     RVTFVDGSFESLM"
     gene            9028..10194
                     /locus_tag="PKX01_09255"
     CDS             9028..10194
                     /locus_tag="PKX01_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76354.1"
                     /translation="MRTSPVPVLIILAALPGLARADAADLLALVQPTRNDAATAVRVD
                     ALGFAADDFTPAHSIARTWNAPPTPRDGPNRALGFARLETSASHGTWSVAVFRRYDAL
                     GSGTPDTVRFYHALAHPDDLLARDQRLAIDYRFTGFSADGLRLTRHFSPAPDQTRYAV
                     ALNLLKAGALRKERATGIATTAGGVATVNGRRSLLYTGLDPTPTSQAHLNDFAPVTTG
                     TPSAGWGVSVDLDARWQPRPDLRLSVATNDLLGRLHWQDVPEMVQDFNGTAWPLQFNQ
                     PQATARITGANHYRDVTLHLKPKVAAAAEWSVSPRWQADASLATVQGVVLPEAGLSWQ
                     ATAGPRVRLSYETRFGSAGVGVRMGPFYAEVRSDRFNLNDAHSLGARAGLTVAF"
     gene            10634..11392
                     /gene="xth"
                     /locus_tag="PKX01_09260"
     CDS             10634..11392
                     /gene="xth"
                     /locus_tag="PKX01_09260"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ76355.1"
                     /translation="MKIATWNVNSLKVRLPHVLDWLAAAQPDALCLQELKCENKAFPL
                     SEIEAAGYHAVFNGQKTYNGVAILSRTPAAEVSLDIPGFADEQKRIIAATVEGVRVVC
                     GYFPNGQAVGSDKFAYKLAWLAALTDWLRGELKTSPRLVLAGDYNIAPEDRDAHPDWK
                     DEIHVSAPEREAFRTLLDLGLSDAFRLFEQADKSFSWWDYRMGAFRRNFGLRIDHLLV
                     SPALKDVCRACYVDKAPRKLERPSDHAPVVVEFA"
     gene            11389..12051
                     /locus_tag="PKX01_09265"
     CDS             11389..12051
                     /locus_tag="PKX01_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ76356.1"
                     /translation="MSTLTPGDELAALYPVLAGLPDAARRRLGNHARRITVPAGTALF
                     DERQACQGFPFVLAGSIRVIKAAPSGRELPLYRVAPGETCIISSACLLGHAAYNARGV
                     TETDTTLLLLPDTDFQSLLAEPAFRDFVFQLFSERIADLMQLIEEVAFRKLDQRLAAL
                     LLGKGQRLHTTHQQLADELGSVREIVSRLLKGFAEQGLVALSREQIEIRDAAGLRRVA
                     AG"
     gene            12272..12463
                     /locus_tag="PKX01_09270"
     CDS             12272..12463
                     /locus_tag="PKX01_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ76357.1"
                     /translation="MKSNVGGIDKILRIGGGVALVAWAALGGPAWAWIGVVPLATGLV
                     GFCPLYPLIGLSTCPMKKD"
     gene            12629..13186
                     /locus_tag="PKX01_09275"
     CDS             12629..13186
                     /locus_tag="PKX01_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HMZ76358.1"
                     /translation="MTKIGLFFGTETGTTRLVAKRLHKQLGDDIADKPVNVNRIEPAE
                     LLRYDALILGTPSYGEGQIPGAGAGCFEPNWEEFLAKMPADADLRGKRVALFGLGAQE
                     RYSERFCSSLFALYQVFKGYGAEIVGAWSTGGYTFQHSAAVVDGQFVGLVIDQRTQGM
                     FTDDRIAQWVAQVKPLLLEKLTAAA"
     gene            complement(13232..14029)
                     /gene="rsmA"
                     /locus_tag="PKX01_09280"
     CDS             complement(13232..14029)
                     /gene="rsmA"
                     /locus_tag="PKX01_09280"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMZ76359.1"
                     /translation="MNAPHLARKRFGQNFLSDPNIIRKIIEAIRPAPDDRMVEIGPGL
                     GAMTEPLMARLDHLHVVEIDRDLIARLKAAHAPDTLTIHEGDALKFDFAALAADGRPL
                     RVVGNLPYNISTPLLFHLADSAERVKDMTFMLQKEVVMRMVAAPGTEDYGRLSVMLQY
                     RFAMARLFDVPPGAFRPAPKVMSSIVRMVPRPLAECTAIDEALLGRIVTAAFGQRRKT
                     LRNTLRDILSDPDFVALGLDPGLRGEALDVAAFVRIANHVAARDAQI"
     gene            complement(14026..14946)
                     /locus_tag="PKX01_09285"
     CDS             complement(14026..14946)
                     /locus_tag="PKX01_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ76360.1"
                     /translation="MRRRALLQAGAALLAASGLSACGGLGTRPDATAKVPAPGLPATP
                     RPRARIGLALGGGAARGFAHVGVIKALETSGIQPDLVVGTSAGAVVGSLYAAGHGPFE
                     LQKLAIQLDESAVTDWSFFDRGFIKGEALERFINTQAGNKPIEGLRRRFAAVATDLQS
                     GEVIVFQRGNTGIAVRASAAIPGVFPPVAVNGREYVDGGLVAPIPVREARSLGADLVI
                     AVDISGRPSGKKNQGSVDVLLDTISIMGGALGKVELAAADVVIRPNLQGLPATNFQQR
                     HEAILEGERAGFAAVTRIREALAAWERKAG"
     gene            complement(14943..15929)
                     /gene="pdxA"
                     /locus_tag="PKX01_09290"
     CDS             complement(14943..15929)
                     /gene="pdxA"
                     /locus_tag="PKX01_09290"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMZ76361.1"
                     /translation="MSDSLPVLAITSGDPAGVGPDLCVALPSRPWAARPVVLGDHELL
                     AARAQMLAPDLAVVPYDPSAAPRDGTLEVLHVPLAVPAEAGRLDAANGRYVLDILDRA
                     IAGCRSGEFAGMVTAPVHKGIICEAGVPFSGHTEYLAEATGTPLVVMMLVGGGLRVAL
                     VTTHLPLKDVPAAITQPLLAEVLRILHADLRERFGLAAPRILVAGLNPHAGEGGHMGR
                     EEIEVIEPVLDALRAEGMDLVGPLPADTLFVPHTLARGDAVLAMYHDQGLPVLKHASF
                     GGGVNVTLGLPIVRTSVDHGTALDLAGTGRADPGSLFAAVELAISLSRGGRP"
     gene            complement(16036..17328)
                     /locus_tag="PKX01_09295"
     CDS             complement(16036..17328)
                     /locus_tag="PKX01_09295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ76362.1"
                     /translation="MRLLALLATLVFTHAAWAAGPVEVDRIVAVVNSDVVTRSDLRLK
                     VEQISRQLTRQGTPLPADDVLERQVLERLIMEKLQIQLANETSLRVDDLALDRALGRI
                     ADNNKLSMTEFRQALEKDGITWDRFREEIRTEMLLSRLREREVESRIVVSDAEVSNFL
                     AHPENTQSQEEFNLAHILLRAPEGATPEQLARLRARAEDVAARVARGEPFDKLAASYS
                     DAPDALSGGNLGWRSAERLPGLFADAVTRMKPGETSPILRSAAGFHIVRLIDRRGGGD
                     ALGATQVQQTHARHILIKTSEVVSDGDARRRLLDLRERIVQGGASFAELARAHSADLS
                     ASKGGDLGWIYPGDTVPEFEQAMDALKPGEISQPVQSPFGWHLIQVEERRVQDVSDER
                     KRNAARNALRERKLDEAYQDWLRQLRDRAYVDIRLEDK"
     gene            complement(17348..19945)
                     /locus_tag="PKX01_09300"
     CDS             complement(17348..19945)
                     /locus_tag="PKX01_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HMZ76363.1"
                     /translation="MKPRFQLRFLSVALFALGGRAGAESLPPFEVPAALLGKPEAIRS
                     RKAAPVPEPAVAAPPVVAAPSTAPAATMPVEPVAATARVATPSARSEAPGAASAAAAV
                     VTAPVPAAGPSAPPQPVAAAVATRSGPAPAPVKNGESVITADKVFGRQDFDVTAEGRA
                     VLLRDGTRVEGDRLQYFELTDEVEATGNVRMTRGDDVMTGPHARFKVQEQVGTFDTPS
                     YEIFRPPRPPRPGQQFDTTQQGRRQPISGHGSAESLELEGENQFRFLNGTWSSCKPDR
                     PDWYLRARELQLDFDREVGEANNGTLYFKDVPVVYLPWADFPLGDQRQSGLLAPSFGT
                     SNKVGFDLAVPYYWNIAPNYDSTITPRLMGRRGLQIRDEVRYMTPTAKGMSFVEWMPN
                     DQVENRSRLAGQIRHNETTPTGWVTAVDVSGVSDPRYFIDLSSRLSLTSQVNLLRQGS
                     VGYYGGGWWNATGMLQAWQTLQDPNGAAVSVPYKRLPQLTLTANRPELPGGTAFLFNS
                     EFVAFSHPTQDEGRRLTLYPQLSLPLVTSAFYVTPKVGVHATEYNLDRRTSTGPDSIQ
                     RSLPILSVDSGVNFERDTQIAGREFTQTLEPRIYYLYVPYRDQSNIPNFDSGVYDFNF
                     AQIFAENYYTGGDRISNANQITTAVQSRLINPNTGAEVIRAAFGQRYYLEEQRVTLPG
                     VAARTGGQADFLAAFGGQIAPRTQLDTGWQYNPRDHWTERFNAGVRYQPGFARALNAS
                     WRYRRNYSTVAATPDGFRDLDLTGQWPLAGNWYAVGRYNRNLRDHRLTEGIAGFEYNG
                     GCWVARTVVQHLTTSATDTTRAFFVQIEFSGLASLGSNPINMLRRSVPGYGKISDPGS
                     SPFFTNDDY"
     gene            20038..21036
                     /locus_tag="PKX01_09305"
     CDS             20038..21036
                     /locus_tag="PKX01_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HMZ76364.1"
                     /translation="MERRQQLQSWLAAEFPGRAFDLAPASADASFRRYYRVTFADDPQ
                     PVIVMDAPPSHEDCRPYLRVAELFGGAGAHVPAVHKQNLEEGFLLLSDLGSTTYLEAL
                     TADNAAHLYADALGALICIQHASRPGVLPDYDRALLLREMELFPVWYVGRHKGVALSD
                     AQSQALYDVFDRIADVNLAEPKVFVHRDFHSRNLMVSTPNPGVLDFQDAVYGPLSYDL
                     VSLFKDAYIHWEEDFVLDMLARYWETARGLGLPVRADFAEFHRDFEWMGVQRHIKVLG
                     IFARLCHRDGKDGYLKDMPLVMHYLRKACERYGELKPLLRLLEQIDPLVTETGYSF"
BASE COUNT         3398 a         7823 c         6692 g         3166 t
ORIGIN      
        1 agtacggcgt gcgcaagatc aacatcgaca ccgacatccg cctcgccatg accggcgcga
       61 tccgcaagtt cttcgtcgag aacccctcca agttcgaccc gcgggagttc aacaagcccg
      121 cccgtgaggc cgccaagctg gtctgcaagg cccgcttcga agccttcggc gccgccggca
      181 atgccagcaa gatcaagccc gtgtccctcg acaagatcgc cgcccgctac aagagcggcg
      241 agctcaacca gatcgtgcgc tgaacccggg cacttcgccc gccgtcggca gtctcaggca
      301 gggccggact ttccggccct gttttcgtct ggaggatccc catggccgtc ccctggtcca
      361 ccctgttcgg cgccatcccc tggtccgacg tcatcgcccg cgcgcccgac atcgcccagg
      421 gcgcccgcaa actctggcag aaggccggcc ggcgcggcga ccctccgccg gacgcaagcc
      481 ccgtcgcgcc gagcctcgac gtccgcgtcg acgcactcgc cgcccgtctc ggcgacatcg
      541 ccgcacgcca gcaggacagc gccgagctgc tcgccgccct ggccactcag caggccgaac
      601 tgatccgcgc caccgacgcc ctgcaccgcc gggcgcgcct gctgggcatc gcctgcgcca
      661 tcctcggcct tgcactggcc ggcctcgcca ccgcgatcct ggtgcgcgcc gcctgatttt
      721 ttgttgcacc gcaacaacag gcgtgacgcc tgccacgcct tgtggttttc ccgaccttgc
      781 cagctcaact tccgtaacgg caagatgggt gccccgatcg cgctccgcgg aggaccgccc
      841 gatgacgccc acgaccctgt ttgccgaaca cgacctgcgc cagaccctca acgacgagtt
      901 ccacgcccgt ccgccggtgc cgctggaaag cccccagctc atcagctacc tggccttcca
      961 ccagcaggac gtggccgccg gcgaagccca gcgccacctg gccgaactgg gtgagcgcct
     1021 gggtcgcccg ctcgatgccg gcggcccgca ctgcctgctg gacttcggcg ccttccagct
     1081 caagtgggag ctccacaacg aattcaccag ctacaccttc atccgccccg tggcgcgggg
     1141 cgaaggactg gtcggcagca cctcggccct ggccgtcgtg ccccaggact ggctgcgcgc
     1201 gatccccggc cgcctgatgg tggccaccca cgtggagctg cgctccgccg ccgaggtgag
     1261 cccggaggag gtgatggccc agctttcgcc caccggccgg cagatggtgg gcgcgatggt
     1321 ggctgacggt gccgcctggg tgttcaccga cttcctcttc gaggacggct tctcgcgctt
     1381 cctcgtcatc gacgtgtccc tcacccgccg ccaggccggc cgcaccgtac agcgcctggt
     1441 ggagatcgag acctaccgcc tgatggccct gctcgccttc ccggtcgcca aggacgtggg
     1501 acgcctcctg ggccgggccg agagcgatct ggcgcgcctc atggaccgca ccggttcggc
     1561 ccgctccccc gaggacgagc gcggcgtgct ggccgacctg tcggccctgg ccgccgaggt
     1621 ggaacactcc gtagcgcgca ccacctttcg ttttggcgcg gcgcgggcct atcacagcct
     1681 ggtcatgcag cgcatcgaag agctgcgcga gcgccgcatc accggcctgc ccaccttcca
     1741 cgagttcatg gaacgccgcc tgctgcccgc catgaacacc tgccagacca tggcgcgccg
     1801 ccaggaagac ctgtccggtc gcgtcgcccg caacagccag ctcctgcgca cccgcgtcga
     1861 ggtggaactg gaacgccaga atcaggaact cctcgcccag atgaaccgcc gcgccaagct
     1921 ccagctccac ctgcaggaga ctgtcgaagg gctgtcggtg gtggcgatca cctactacgc
     1981 gtcgcagctc gtgcagtaca tggccaaggg cgtcaagcac ttcatcgagc ccctcacgcc
     2041 ggaaggcctc accgccgccg ccatcccggt gatcgccggc atcgtcgccc tgggcctgca
     2101 ccgcatgcgc aaggcgcttg ccgccgccga cggcggtcac tgaacacctc cgccgcaccg
     2161 gggccaccgg gtggccgtgc cggcgggccg ggctataatc gcgttttgtc cgccgggatc
     2221 ctcaccgtga ccacgcctct cttcgaatcg tccctgcgca gcctgcccct gctcggccgc
     2281 ggcaaggtgc gcgacatcta cgccgtcgac gccgaccggc tgctcatcgt cacgtccgac
     2341 cgcctgtccg ccttcgacgt gatcctgccc gacccgattc cgcgcaaggg cgaagtgctc
     2401 accgccatgg ccaacttctg gttcgcccgg ctcggccaca tcatccccaa ccagctcacc
     2461 ggggtcgatc cggaaagcgt ggtggccgcc gacgagcgcg accaggtgcg cggccgcgcc
     2521 ctggtggtca agcgcctgcg tcccctgccc atcgaggcgg tggtgcgcgg ctacgtgatc
     2581 ggctcgggct ggaaggacta ccaggacacc ggcgccatct gcggcatcgc gctgcccgcc
     2641 ggcctcaagc aggcccagaa gctgcccgcc ccgatcttca ccccggccag caaggccgac
     2701 gtgggcgacc acgacgagaa catctccttc gaacaggccc aggcccgcac tgctgcccag
     2761 tttgccgaca gtggcctgga cggcgcccgc ctggtgagtg aagcccgtga cgccgccctc
     2821 gccctctact cggaagcgtc ggcctacgcc gccggccgcg gcatcctcat cgccgacacc
     2881 aagttcgaat tcggcgtgga cgccgccggc accctgcacc tcatcgacga ggccctgacg
     2941 ccggactcct cccgtttctg gcctgccgac agctacgccg agggcatcag tccccccagc
     3001 ttcgacaagc agtacgtgcg tgattacctg gaaaccctcg actggggcaa gaaggcacct
     3061 ggcccgacgc tgcctgcgga cgtcatcgcc cgcacctcgg ccaagtatgt ggaagcctac
     3121 gagcgcctca ccggtctcag cctgtaagcc ccacgcagcc gctcccggcc ggcgccgggg
     3181 cggcgcgcct cacaggtgga tgctgaagca ggcgatcagc gcctcccgct cttccgcaga
     3241 ctccttcaac accgcgccga tctccacatc gtccagcccc agggtcgcca ccttggccag
     3301 accgttgcgg aagggctccg gctccagaga gaacggattc cccgtctcgg ccagcaggtt
     3361 ggcgatgaag atcaggtccg ccacattctc cggcggccag cgtccgccgt agagttccgg
     3421 atcgtccacc gcatccacca gatcctgggg catgtgcagg gaggccagca cggcccgccc
     3481 gatgggcgga tgccacaccc gcaccagatc cgacagggga ctgccctccc gcagcagctc
     3541 cgggtattcc cacacccgcg cgagcaggaa gaactggccg atgtcgtgga ccatgcctgc
     3601 gaagaaggcc tcgtccgggt tgatccggct gcaatgccgc gccagcacgc aggcggtggc
     3661 cgccacatcc acgcaatggg cccacagcac ctgcatctgg ggctggaagg ggcccagcat
     3721 gtccttggtg gcgagctgtt cggccgccac ggtaagcgcc agggcccgca gtttggcgat
     3781 tccgatgcgc gccagcgccc gtggcacgtc cgccaccagc cggccccccg ggttgtacca
     3841 ggcggagttg gccagcgcca cggtgcgcgc cgccagcagg ggctcggccg acaccatgcg
     3901 ggcaatgcgc gcaatgtccg cgtccggatc gtcgagcacc tccatgaccc gcagggtcac
     3961 ttccatcgcg gtgggaaact tcagttcccc gcgttccagc tccatcgtga tggtgtgcag
     4021 aaaagcctgt tccaccaggc tcagttccga ttcatccatg ggaccgccac aaaccttccc
     4081 gccgacccgc gacaccccgc acggaattcg gcacaataaa aagatccacc ccaccgacac
     4141 gcaacaatga ccaccgccac tgctccgtct cccctgctcc agcccaccgg cctgccgcgc
     4201 ttcgacgcca tcacgcccga ccaggtcgaa cccggcctgc gcagcctcct cgacgcctac
     4261 ggcgccaccc tcgcccgcct gcaggacgat ccgacgacgc ccacctgggc gaatttcgtc
     4321 gaacccatcg agctcgaagg cgagcgcctc ggccgcgcct ggggcgtggt ggtgcacatg
     4381 cacagcgtga tggacacccc ggaatggcgc gaggcctaca acgcaatgtt gccggaggtc
     4441 acccgctttt acgccgaatt ggggcaaaac cttaagcttt tcgaaaaata caaggctatc
     4501 gccgaaggtc ccgaattcgc cggcctcacg cccgcccgcc agcgcatcct cgacaacgag
     4561 ctgcgcggct tccgcctttc cggcgccgaa ctcccggacg cccggaagcc ccgcttcaag
     4621 gaaatccagg aagaactggc cggcctctcc gccaggttct ccgaaaacct cctcgacgcc
     4681 accaacgcct tcagccacct cgtcaccgac ccggcccgcc tcgccggcct gccggacgac
     4741 gtagtgaccg cggcccgcga agcggccgcg aaggccggcc aggaaggctg gaaattcacc
     4801 ctcaagatgc cctcctacct gccggtcatg cagtacgccg acgaccgcgc cctgcgcgaa
     4861 acgatgtacc gcgccaacgg cacccgcgcc gccgagatcg acaacggctt cagcaagccc
     4921 gagtggaaca acggcccgct gatcgagcgc atcctcgccc tgcgtgccga agaagccgcg
     4981 atgctcggct accgcaccta cgccgacgtc tccctggtgc ccaagatggc cgactccccc
     5041 gagcaggtgc tcggcttcct gcgcgaactg gccgccaagg ccaggccctt cgccgagcgg
     5101 gacctggccg aactcgccac ctttgcccgc gacgaactgg ggctcgaccc cctcgaaccg
     5161 tgggacacgg gctacgcctc cgaaaaactg cgggtcgccc gctacgactt ttccgagcag
     5221 gaggtgaagc agtacctgcc cgaacccaag gtcctgcagg gactcttcga cgtcgtggag
     5281 aagctctacg gcgtgcgcat cctgcccgac agcggcccgg tgtggcaccc ggatgtgcgc
     5341 ttcttccgca tcgagcgcca gggcgcgctg gtcggccagt tctacctgga cctctacgcc
     5401 cgcgagacca agaagggcgg cgcctggatg gattccgcca tcagccggcg gcgcctcggc
     5461 gacggcatcg agacgccggt cgcctacctg gtgtgcaact tccccggccc ggtcggcggc
     5521 aaggctgcca ccttcagcca cgacgacgtg atcaccctct tccacgaggc cggccacggt
     5581 ctgcaccatc tcctcacgcg ggtggacgac ctggccgtct ccggcatcca cggcgtcgaa
     5641 tgggatgccg tcgagctgcc cagccagttc atggagaact tctgctggga atgggacgtg
     5701 gtccgcgaga tgaccgccca cgcggacacc ggcgaggccc tgccccgcgc cctcttcgac
     5761 aagatgctcg ccgccaagaa cttccagagc ggcatgcaga ccgtgcgcca gctggaattc
     5821 gccctcttcg acctgcgcct gcacctggac ttcaacccgg ccaacccgcg cgtcatggac
     5881 ctcctcgacg aagtccgcca ggaagtggcc gtgctcgtcc cgcccgcctg gcaccgcttc
     5941 cccaacagct tcagccacat cttcgccggc ggctacgcag cgggttacta cagctacaag
     6001 tgggccgagg tgctctccgc ggacgcgttc gccgccttcg aggaagccgg ccggccccag
     6061 ggcagcgtgc tggacgccgt caccggcgcg cgcttctggg aagagatcct cgcggtcggc
     6121 ggcagccgtc cggcgctgga gtccttcaag gccttccgcg ggcgcgaacc ccaggtggac
     6181 gccctcctgc gccacagcgg catggtgccc gcatgaaacg ccgtagcgcc ctcctcctgc
     6241 tgatcgccgc cgcgccggcc ctcgcccaga ccgcctatcg ctgggtggac gacaagggcc
     6301 gcgtccagta cagcgaccag ccgccccccg cggcggtgcc ccagtacgac cagcgcaaca
     6361 tcagcgccaa ccagagcggc agcctccagc aatccttcgc cacccgccag gcggccggca
     6421 aatacccggt cacgctgtac gtctccaagg attgcggcaa ggcctgcgag gacgggcggg
     6481 cattcctcaa gaagcgcacg atccccttca gcgagcagcg catcgaaagc gccgaggacg
     6541 tcgcgaccta caaggcccgc atcggtgcag agcccttcgt accgaccctc ctggtggggc
     6601 gcgagaagga gaccggcttc gctgcgcccg cctgggacaa tctgctcgac aaggccggct
     6661 accccgccgc acgccgctga cgcaccgttc tgcgacgccc gcggacccgc gccgcgggcg
     6721 gacgacagac attgcctgcc tcagccggca gatccggtgc ctttccctcc ggaataagcc
     6781 tttcgtgcta tgactttcgt tcaattgtca taacacgtat aactctatac agtctccacc
     6841 tcccgatcgt catggagact gccagtgaaa accagaaacc gcctgattgc cgaagccctg
     6901 gggggcgccc tgttcctgtc cgcctgcggc ggcggcggag ggggcagcag caccccggcc
     6961 agcccccaga cgccggccag cgtcgacgtc accgtcaccc cgtcccttgg caagttctcc
     7021 accggctgca gcgtcgagct gcgcaccggc agcgggacgc tcctcggttc ggcgcccatc
     7081 accgacgccg gcaacgcctt catccgcgtc accggctaca gcggcccggt catcgcccag
     7141 gtcaagggcg ccagcggctg cacctacttc gacgaagcca ccgccaccca ggtccccttc
     7201 ggcgccgacc gcagcctgag cgccgtctcc gacaccctgc gggtggacca gggcatcaac
     7261 gtcctcaccc acctggccgc cagccgcgtg ctcgacggcg acaagctcgc caccggcaag
     7321 accgccgaca ccgtcaagct ggaaaacctc accatccagc gcatgttcca ggtggccgac
     7381 atgttcgcgg cgcccacgct gctgggcaac gcctcagacc ggctcgacaa caccgcggcg
     7441 ggccagcttg ccgtgcagct cgccgccctc tccgaactgg cccacacccg cgccacggac
     7501 gtcgccaccc tcgccgccac gctggccacc gacctcgccg acggcaccct cgacaccttt
     7561 ggcgccagcg aataccaggt cgccgcgcag accaccgtcg ccaagctggc ctccccgtcg
     7621 gcccaggcct ccctcctcat cctgagccag acccccagcc tcatcgtgag cacccgcgat
     7681 gtgcagtcgg acgtgcagaa ggtcatcgcc gccggcccgg ccctcgccca ggcgaagcag
     7741 ctcttcgccg acctgcgcac cagcgtgctc gccctcagca acagcgccgg caccggcacc
     7801 ctcgacgccg aaaacgcact gctcaaggcc gacttccagc acggcctcgg cgtggacatg
     7861 ctgatcgacg gcgtcaaatt catgctcgaa tccgccagcc agttcttcga ttccggcacc
     7921 tccaggcccg gcagcaccca tagcggctgg gtcggcacct gctaccagga ctccacgacc
     7981 caagccacct gccatgtttc gggcgtagac gacggcagcg tctacaccag ctaccaagtc
     8041 cgcctgacca agaccggcgc cggtacggcc tcgtgggcct tcacacaggt gtaccgctac
     8101 cagaacgggc agagcaccca gaccgccgcg ccctccggca tgagcggcag cttcacgtac
     8161 tccgccaatg ccgccacggc caaggggcag ttcatcccca tgaccggcga cgccgagcgc
     8221 agcgccatcg acatcagcct cggcttcact accacaggga ccaccggcgc catccgattc
     8281 agcgccaccg gctcggccaa ttcggtcaag accgacggca ccagcaccct ccaggtggat
     8341 ctcaccaagc tcgacctgga cccggtcagt caggtgaacc agatggtgtt gagcctcacc
     8401 ggcccccgtc accgcttcga cggcgcactc accctgagcg gccaggtggc ctcggccgat
     8461 ggcagcatcg cccagcccaa gtcgctcaag ttcgtcggca ccttcaccga cactgcaagc
     8521 cactacacgg tcctcgacgg caccgtcacc gcatcccagg actggaccgg ctacgacccc
     8581 cgcctggcca actccgacgg caacttcgcc cgctatgcct tcagctttga cggcgtcgcc
     8641 aacaagggac cgggtgcggc ggggatcacc ctgtccttca gcggcgacaa ccaggcaggg
     8701 ccccacgacc agaagatcgc gctcaagttc accagcgcca gcggcatcgt cgtgaccggc
     8761 accggcgtcc agacgccggc acgccccgac gacttgtgga actggacctt cgccaacgag
     8821 aacggcatca aggcgactta cgcccagagc agccggtccg gcaaggtcac ggccggcgat
     8881 ggcagcgacc tgggcaccct gagcagccag cgtgtcacct tcgtggacgg cagtttcgaa
     8941 tcgctgatgt aagcccatcg tcctcccggc cggctggcgc cttcgcccgc cggccgcttt
     9001 ccgtccttct ctcatgacgc ccgctccgtg cgcacttccc cagtccccgt cctgatcatc
     9061 cttgccgcgc tgcccggcct cgcccgggcc gacgcggccg accttctggc cctggtccag
     9121 ccgacgcgca acgatgccgc caccgccgtg cgggtggacg cactgggctt cgccgccgac
     9181 gacttcacgc ccgcccacag catcgccagg acctggaacg cgccgcccac cccacgggac
     9241 ggccccaacc gggccctcgg attcgcccgc ctcgagacct ccgcctcgca cggcacctgg
     9301 agcgtcgccg tcttccgtcg ctacgatgcc ctgggcagcg gtacgcccga tacggtgcgc
     9361 ttctaccacg ccctcgccca cccggacgat ctgctcgccc gcgaccagcg cctggccatc
     9421 gactaccgct tcaccggctt ttcggccgac ggcttgcgcc tgacccggca cttctccccc
     9481 gcgcccgatc aaacccgtta cgccgtcgcc ctcaacctgc tcaaggccgg cgccctgcgc
     9541 aaggagcggg ccactggcat cgccaccacc gccggtggcg tggccaccgt caatggccgt
     9601 cggagtctgc tgtacaccgg cctcgatccg acaccgacat cccaggccca tctcaacgat
     9661 ttcgcccccg tcaccaccgg tacgccgagc gcaggctggg gggtcagcgt ggacctggat
     9721 gcccggtggc agccccgtcc ggacctgcgc ctgagcgtcg ccaccaacga tctcctcggc
     9781 cgcctgcact ggcaggacgt gcccgaaatg gtccaggact tcaacggcac cgcctggccg
     9841 cttcagttca accagcccca ggccaccgcc cgcatcacgg gcgccaacca ttaccgggac
     9901 gtcacactgc acctcaagcc caaggtggct gccgccgcag aatggtcggt cagcccgcgc
     9961 tggcaggcag acgccagcct cgccaccgtt cagggtgtgg tgctgcccga ggccggcctg
    10021 agctggcagg ccaccgcagg gccccgcgtg cggctgtcct acgagacccg cttcggcagc
    10081 gccggcgtgg gcgtccgcat gggccccttc tacgccgaag tccgcagcga ccggttcaat
    10141 ctcaatgacg cccacagcct tggcgcacgg gccggcctga cagtggcatt ctgaggcgcc
    10201 acgccccaga cacccgcgcc gacctgtcgt gacgcgatcc tgaaaccccc gcatcttcaa
    10261 ggcgttttac cctgaatccc ggcctgcccg cgtcatcgac accacccgga atggccgcac
    10321 ctgcgatcgc cccgcctggc gcccctgtcc gccctcgccc ccagtcagca ggctggcatc
    10381 gaacagacct caggcgcctc cacaccggac gcaccctcgc ccggcgagcc cttcacctgc
    10441 cgatgtctgt gcccctgggc gactcgagcg cgaccggtca cgccatgccc gacgacgccc
    10501 cctcgccatc caccgcgttg ccgtatcctc aagccgacgt accgctggca caatcacgtc
    10561 cattcccggt ccgccgaaac ccggcggcgc catttttccg cggctcgtcc gcacagcccg
    10621 acgccccgcc cccatgaaaa tcgccacctg gaacgtcaat tccctcaagg tccgcctgcc
    10681 ccacgtgctc gactggctgg ccgcggcgca gccggacgct ctgtgcctgc aggaactcaa
    10741 atgcgagaac aaggcctttc ccctctccga gatcgaggcc gcgggctacc acgccgtctt
    10801 caacggccag aagacctaca acggtgtcgc catcctcagc cgcacgccgg ccgcagaggt
    10861 cagcctggac atccccggct tcgcagacga gcagaagcgc atcatcgccg ccaccgtcga
    10921 gggcgtgcgc gtcgtgtgcg gctactttcc caacggtcag gccgtcggct ccgacaagtt
    10981 cgcctacaag ctggcctggc tcgcggccct caccgactgg ctgcgcggcg agctgaagac
    11041 gtcaccgcgc ctcgtgctgg ccggcgacta caacatcgcc ccggaagacc gggacgccca
    11101 cccggactgg aaggacgaga tccacgtctc cgccccggag cgggaggcct tccgcaccct
    11161 gctcgacctg ggcctgagcg acgccttccg cctgttcgag caggcggaca agagcttctc
    11221 ctggtgggat taccgcatgg gcgccttccg tcgcaacttc ggcctgcgca tcgaccacct
    11281 gctggtctcg cccgcactga aggacgtgtg ccgtgcgtgc tacgtggaca aggccccgcg
    11341 caagctggag cgcccctcgg accacgcccc ggtcgtcgtc gaattcgcat gagcaccctc
    11401 acgccgggcg acgaactggc cgccctctac cccgtcctcg ccggtctccc ggatgccgcc
    11461 cgccgccggt tgggcaacca cgcgcgccgc atcacggtgc ccgcaggtac cgccctcttc
    11521 gacgagcgcc aggcctgtca gggcttcccc ttcgtcctcg ccggcagcat ccgggtcatc
    11581 aaggccgcgc ccagcggccg cgagcttccc ctctaccggg tggcgccagg ggagacctgc
    11641 atcatctcct cggcctgcct gctcggccat gccgcctaca atgcccgcgg cgtcaccgag
    11701 accgacacca cattactgct cctgcccgac actgacttcc agtccctgct cgccgaacca
    11761 gccttccgcg acttcgtctt ccagctcttc tccgagcgca tcgccgacct gatgcagctc
    11821 atcgaggaag tggccttccg caagctcgac cagcgcctcg ccgccctgct cctgggcaag
    11881 ggccagcgcc tgcacaccac ccatcaacag ctcgccgatg aactgggcag cgtgcgcgag
    11941 atcgtcagcc gcctgctcaa gggcttcgcc gaacagggcc tggtggcgct gtcgcgggag
    12001 cagatcgaga ttcgcgacgc tgccggcctg cgccgcgtcg ccgccggctg acgcccggcc
    12061 cgaatgcatc ggacggcccg gcgtcctgca ggcgccgcag cggacgatgt cccgcccccg
    12121 gctgccctgc gcgccgtgtc catgcccgtc cgttcaaacc aatcaccggc actgccaccg
    12181 gcacccgccg cctctgtaac tgaggttaca gaccgcgctt ccgcctcctg ctgaaatacg
    12241 ctcacctgac aacccttccc cggaggacat catgaagagc aatgtgggcg gtatcgacaa
    12301 aatcctgcgt attggcggcg gcgtggccct ggtggcctgg gcggccctcg gcgggccggc
    12361 atgggcctgg atcggcgtgg tgcccctggc caccggcctg gtgggcttct gcccgctgta
    12421 cccgctgatc ggtctcagca cctgcccgat gaagaaggac tgacccctgc agggcgcccc
    12481 ttgggcgcct ttcggtccat cgcgcggggg cgctccggaa gtcaccccgc gcgtgggtac
    12541 agccggcgcc gagggcctca tcgagccccc aggccgacgg cgcgaaactt gcatggacag
    12601 tgcatcgtcc ttccgtttcg tcgcagtcat gacaaagatc ggcctcttct tcggcaccga
    12661 aaccggcacc acccgcctgg tcgccaaacg tctgcacaag cagcttggcg acgacatcgc
    12721 cgacaaaccc gtcaatgtga accgcatcga gccggccgag ttgctgcgct acgacgcgct
    12781 gattctgggc acgcccagct acggcgaagg ccagattccc ggcgccggcg ccggctgctt
    12841 cgagcccaac tgggaagaat tcctcgccaa aatgcctgcc gacgccgacc ttcgcggcaa
    12901 gcgggtggcc ctctttggcc tgggcgccca ggagcgctac tcggaacgct tctgcagctc
    12961 cctgttcgcc ctctaccagg tcttcaaggg ctacggcgcc gagatcgtcg gcgcctggag
    13021 caccgggggc tacaccttcc agcactccgc cgccgtggtg gacgggcagt tcgtgggcct
    13081 ggtgatcgac cagcgcaccc agggcatgtt caccgacgac cgcatcgccc agtgggtggc
    13141 gcaggtcaaa ccactgctgc tggaaaagct caccgcagcc gcctgaccct gctccgggtt
    13201 gccgccgggc cgctccggcg gcggcacgcg ttcagatctg cgcgtcacgc gccgccacgt
    13261 gattggcgat gcgcacgaag gctgccacgt ccagcgcctc gccgcgcagg cccggatcga
    13321 ggccgagggc cacgaagtcc ggatcggaca ggatgtcccg cagcgtgttg cgcagggtct
    13381 tgcggcgctg gccgaaagcc gcggtgacga tgcgtccgag cagggcctcg tcgatggcgg
    13441 tacattccgc cagcggccgc ggcaccatgc gcacgatgct ggacatcacc ttgggcgcgg
    13501 gccggaaggc gccgggtggc acgtcgaaca ggcgggccat cgcaaaacgg tactggagca
    13561 tcaccgacag gcggccatag tcctcggtgc ctggcgcagc caccatgcgc atcacgactt
    13621 ccttctgcag catgaaggtc atgtctttca cccgctcggc gctgtcggcc agatgaaaga
    13681 gcaggggcgt ggagatgttg tagggcagat tgcccaccac ccgcagggga cggccgtccg
    13741 ccgccagggc ggcgaagtcg aacttcagcg catcgccctc gtggatggtg agggtgtcgg
    13801 gcgcgtgggc cgccttgagg cgcgcgatca ggtcccggtc gatttccacc acatgcaggt
    13861 ggtcgaggcg cgccatcagg ggctcggtca tggcccccag acccggcccg atctccacca
    13921 tgcggtcgtc cggtgcgggc cggatcgcct cgatgatctt gcggatgatg ttggggtcgg
    13981 acagaaagtt ctggccgaag cgcttgcgcg ccagatgggg tgcgttcatc ccgccttcct
    14041 ttcccaggcc gccagggcct cgcggatgcg cgtcacggcg gcaaagccgg cccgctcccc
    14101 ttccaggatg gcctcgtgac gctgctgaaa gttggtggcc ggcaggcctt gcagattggg
    14161 acggatcacc acgtcggccg cggccagctc gaccttgccg agggcgccgc ccatgatgga
    14221 aatggtgtcc agcagcacgt ccacgctgcc ctggttcttc ttgccgctgg gccggccgga
    14281 gatgtccacg gcgatcacca ggtcggcacc caggctgcgc gcctcacgca ccgggatggg
    14341 tgccaccagc ccgccatcca cgtattcccg gccattgacc gcgacgggcg ggaagacgcc
    14401 gggaatcgcc gccgaggcgc gcacggcgat gcccgtgttg ccacgctgga agacgatcac
    14461 ctcgccgctc tgcagatctg tggccactgc cgcaaagcgg cggcgcagcc cttcgatggg
    14521 cttgttgccg gcctgggtgt tgatgaagcg ttccagcgcc tcgcccttga tgaagccgcg
    14581 gtcgaagaag ctccagtcgg tcaccgccga ttcgtcgagc tggatggcga gcttctgcaa
    14641 ctcgaagggc ccgtgacccg ccgcgtagag actgccgacc acggcaccgg cgctggtgcc
    14701 caccaccagg tccggctgga tgccgctcgt ttcgagggcc ttgatcaccc ccacgtgggc
    14761 aaagccgcgg gccgcaccgc cgcccagggc caggccgatg cgcgcacggg gacggggcgt
    14821 cgcgggcaaa ccgggcgccg gcaccttcgc ggtggcgtcc ggccgggtcc ccagcccccc
    14881 gcaggctgac agacccgagg ccgccagcag cgccgcaccc gcctgcagca gcgcgcgccg
    14941 cctcatggcc ggcctccgcg gctcaggctg atggccagtt cgaccgcggc gaacaggctg
    15001 cccggatcgg cgcgccccgt gccggcgaga tccagggccg tgccatggtc caccgaagtc
    15061 cgcacgatgg gcaggccgag ggtgacattc actccgccgc cgaagctggc atgcttgagc
    15121 accggcaggc cctggtcgtg gtacatggcg agcacggcgt cgccgcgggc gagggtgtgg
    15181 ggcacgaaga gggtgtcggc cggcagggga ccgacaaggt ccatgccctc ggcgcgcagg
    15241 gcgtcgagga ccggctcgat gacctcgatc tcctcacggc ccatgtggcc accctccccg
    15301 gcgtgggggt tgaggcccgc caccaggatg cgcggcgcgg ccagtccgaa gcgctctcgc
    15361 aggtcggcgt ggaggatgcg cagcacctcg gcaaggaggg gctgcgtgat cgcggcgggc
    15421 acgtccttga ggggcaggtg ggtggtgacc agcgccaccc gcaggccgcc acccaccagc
    15481 atcatgacga ccaggggcgt gccggtggct tcggccaggt attcggtgtg ccccgagaaa
    15541 ggcacgcctg cctcgcagat gatgcccttg tgcacggggg cggtgaccat tccggcgaac
    15601 tcgccgctgc ggcagcccgc gatggcccgg tcgaggatat ccagcacgta gcggccgttg
    15661 gcggcgtcca gacggcccgc ctctgcgggg actgccaggg gcacgtggag gacttccagg
    15721 gtgccgtcac ggggggctgc ggacgggtca taggggacga cggcaagatc cggcgccagc
    15781 atctgcgccc gcgcggcgag cagctcgtga tcgccgagga cgaccggacg ggccgcccag
    15841 ggccgggacg gcagggcgac acacaggtcc ggcccgaccc cggcgggatc gccgctggtg
    15901 atggcaagga cggggaggga atcagacatg gggcttcaag aaaattgccc gccggaaagg
    15961 cgggcggaag gatcacgacg gaaatgactc aagcgagctg tgagactgtg atccggcctc
    16021 cgcgttcccg ccggatcact tgtcctcgag gcggatatcc acgtaggccc ggtcgcgcag
    16081 ctggcgcagc cagtcctggt aggcttcgtc gagcttgcgc tcacgcaggg cattgcgggc
    16141 ggcgttgcgc ttgcgctcgt cggagacatc ctgcacacgc cgctcctcca cctggatcag
    16201 gtgccagcca aagggcgact ggaccggttg ggagatctcg ccgggcttga gggcgtccat
    16261 ggcctgttcg aattcgggca ccgtgtcgcc gggatagatc cagcccaggt cgcccccctt
    16321 ggaggcggac aggtcggcgg aatgggcgcg ggccagttcg gcgaaactgg cgcccccctg
    16381 aacgatgcgc tcgcgcagat ccagcaggcg gcggcgggca tcgccatccg acaccacttc
    16441 gctggtcttg atgaggatgt ggcgggcatg ggtctgctgc acctgggtgg cgccgagggc
    16501 atcgccgccg ccgcgacggt cgatcaggcg gacgatgtgg aagccggcag cactgcgcag
    16561 gatgggcgag gtttcgccgg gcttcatccg ggtaaccgca tcggcaaaca ggccgggcag
    16621 gcgctcggcg ctgcgccagc ccaggttgcc gccggacagg gcgtcgggcg catcggaata
    16681 gctggcggcc agcttgtcga agggctcgcc gcgggccacc cgggccgcca catcctcggc
    16741 acgggcacgc aggcgggcga gttgctcggg ggtggcgcct tccggcgcgc gcagcaggat
    16801 gtgggcgagg ttgaattctt cctggctctg ggtgttttcg ggatgggcga ggaaattgct
    16861 cacctcggca tcggagacga cgatgcgcga ctccacctcc cgctcgcgca ggcggctgag
    16921 gagcatctcg gtgcggatct cctcgcggaa gcggtcccag gtgatgccat ccttctccag
    16981 cgcctgacgg aattccgtca tggacagctt gttgttgtcg gcgatgcgcc cgagggcccg
    17041 gtcgagggcc agatcgtcca ccctcaggga ggtctcgttg gccagctgga tctgcagctt
    17101 ttccatgatc aggcgttcga gcacctgacg ctccagcacg tcgtcggccg gcaggggcgt
    17161 gccctggcgg gtgagctggc ggctgatctg ctcgaccttg aggcgcaggt cggagcgggt
    17221 gaccacgtca ctgttgacca ccgccacgat gcggtccacc tcaaccggtc cggccgccca
    17281 ggcggcatgg gtgaagacga gcgtggcaag cagggcaagg agacgcatca tgagacggcc
    17341 cgattgatca atagtcatcg ttggtgaaga aggggctgga gccggggtcg ctgatcttgc
    17401 cataacccgg cacgctgcgg cggagcatgt tgatggggtt ggagcccagg ctggcgagcc
    17461 cgctgaactc gatctgcacg aagaaagcgc gggtggtgtc ggtggcggag gtggtcagat
    17521 gctgcacgac ggtgcgcgcc acccagcagc caccgttgta ctcgaagccg gcgatgcctt
    17581 cggtgaggcg gtggtcccgc aggttgcggt tgtagcgccc cacggcgtac cagttgccgg
    17641 ccaggggcca ctgcccggtg aggtccaggt cgcggaaacc gtcgggggtg gcggccacgg
    17701 tggaatagtt gcggcgataa cgccagctgg cgttgagggc gcgggcaaag cccggctggt
    17761 agcgcacgcc ggcattgaag cgctcggtcc agtggtcccg cgggttgtac tgccagcccg
    17821 tgtccagctg ggtgcgcggc gcgatctgcc cgccaaaggc ggcgaggaag tcggcctgcc
    17881 cgccggtgcg ggcagccacg ccgggcaggg tgacgcgctg ctcttccaga taatagcgct
    17941 gcccgaaggc ggcccggatg acctcggcgc cggtgttggg gttgatcagc cggctctgca
    18001 ccgcggtggt gatctggttg gcgttggaga tgcgatcacc gccggtgtag tagttctccg
    18061 cgaagatctg cgcgaagttg aagtcgtaca ccccggagtc gaagttgggg atgttggact
    18121 ggtcgcggta gggcacgtag aggtaataga tgcgcggctc gagggtctgg gtaaattcgc
    18181 gccccgcaat ctgtgtgtcc cgctcgaaat tcacgccgct gtccaccgac aggatgggca
    18241 ggctgcgctg gatggaatcc ggcccggtac tggtgcgccg atccaggttg tattcggtgg
    18301 catgcacgcc gaccttgggt gtgacataga acgcggacgt gaccagcggc agggacagct
    18361 gcgggtacag cgtcagacgc cggccctcgt cctgtgtcgg atggctgaag gccacgaatt
    18421 cgctgttgaa caggaaggcg gtgccccccg gcagctcggg ccggttggcc gtgagggtca
    18481 gctggggcag gcgcttgtag ggcacggaga cggcggcccc gttgggatcc tgcagggtct
    18541 gccaggcctg cagcatgccg gtggcgttcc accagccgcc accgtaatac cccacgctgc
    18601 cctgacgcag caggttgacc tgggaggtga gggacagccg ggagctgaga tcgatgaagt
    18661 agcgcggatc ggacacgcca ctcacgtcca cggcagtcac ccagccggtg ggcgtcgtct
    18721 cgttgtgacg gatctgcccg gcgaggcggc tgcggttctc gacctgatcg ttgggcatcc
    18781 attcgacgaa ggacatgccc ttcgcggtgg gcgtcatgta gcgcacttcg tcgcggatct
    18841 gcagtccgcg gcgccccatc aggcggggcg tgatggtgga atcgtagttc ggggcgatgt
    18901 tccagtaata cggcaccgcc aggtcgaagc ccaccttgtt ggacgtgccg aaactgggcg
    18961 ccagcaggcc ggactggcgc tggtcaccca gcggaaaatc ggcccagggc aggtagacga
    19021 ccggcacatc cttgaagtac agcgtgccat tgttggcctc gcccacctcc cggtcgaagt
    19081 cgagctggag ttcccgggcg cgcaggtacc agtccggacg gtccggcttg caactgctcc
    19141 aggtgccatt gaggaagcgg aactgatttt cgccttccag ctcgagggac tccgcggatc
    19201 catgcccgga gatgggctgg cgccgcccct gctgggtcgt gtcgaactgc tgaccgggcc
    19261 tgggcgggcg gggcgggcgg aagatctcgt agctgggggt atcgaaggtt ccgacctgtt
    19321 cctgcacctt gaagcgcgcg tggggcccgg tcatcacatc gtcaccgcgg gtcatgcgga
    19381 cgttgcccgt cgcctccacc tcgtcggtga gctcgaagta ctggagccgg tcgccttcca
    19441 cacgggtacc gtcccgcagc agcacggcgc gcccttcggc ggtgacgtca aaatcctggc
    19501 gcccaaacac cttgtcggcg gtgatcacgg attcgccatt cttgaccggg gccggcgcag
    19561 ggccggagcg ggtcgccacc gcagcggcca ccggttgcgg cggcgcggag ggtcccgccg
    19621 ccgggacggg tgcggtcacg actgccgcgg ccgcagacgc ggcaccgggc gcctccgacc
    19681 gcgcggacgg cgtcgcgact cgcgccgtcg ccgcaaccgg ttcgaccggc atggtcgccg
    19741 ccggcgctgt ggacggcgcg gcgaccaccg gcggcgccgc gacggcgggt tcggggactg
    19801 gcgccgcctt gcgggaacgg atcgcctccg gcttgcccag cagggcggcc ggcacctcga
    19861 agggggggag gctttcggct cctgcgcgcc ctcccagggc gaacagggcg acggacagga
    19921 aacgcaactg gaaacgtggc ttcatccgga ggctggcacg gtgacggcga gcggcatcga
    19981 gccggtcgcg cggtttgata aacttgccaa ttttacacga acgataccct gactcccgtg
    20041 gaacgacgcc aacaactcca gtcctggctt gccgccgaat tccccggccg cgccttcgat
    20101 ctcgccccgg ccagtgccga cgccagcttc cggcgctatt accgcgtcac cttcgccgac
    20161 gatccgcagc ccgtgatcgt catggacgcg ccccccagcc acgaagactg ccgcccctac
    20221 ctgcgcgtcg ccgagctctt cggcggagcc ggcgcccacg tgcccgccgt gcacaagcag
    20281 aacctcgaag agggcttcct gctgctctcc gacctgggct ccaccaccta cctggaggcc
    20341 ctcaccgccg acaacgccgc ccatctctac gccgacgccc tcggcgcgct gatctgcatc
    20401 cagcacgcca gccgccccgg cgtactgccc gactacgacc gggcgctgct gctgcgcgag
    20461 atggagctct tccccgtgtg gtacgtgggc cgccacaagg gcgtcgccct cagcgacgcg
    20521 cagtcccagg ccctctacga cgtcttcgac cgcatcgccg acgtcaatct ggccgaaccc
    20581 aaggtcttcg tgcatcggga tttccacagc cgcaacctga tggtgtccac gcccaacccc
    20641 ggcgtgctgg atttccagga cgccgtctac gggcccctca gctacgacct ggtgtccctg
    20701 ttcaaagacg cctacatcca ctgggaagaa gacttcgtcc tcgacatgct cgcccgctac
    20761 tgggaaaccg cccgcggcct gggcctgccc gtgcgcgccg atttcgccga gttccaccgg
    20821 gacttcgaat ggatgggggt gcagcgccac atcaaggtcc tcggcatctt cgcccgcctc
    20881 tgccaccggg acggcaagga cggctacctg aaggacatgc ccctggtcat gcactacctg
    20941 cgtaaggcct gcgaacgcta cggcgagctc aagcccctgc tgcgcctgct ggagcagatc
    21001 gacccgctcg tcaccgagac cggttattcc ttctgaacca tgaaagcgat gatcctcgcc
    21061 gccggccgcg gcgaacgca
//
LOCUS       DAOQPA010000055        20942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1229_length_20942_cov_20.425671, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000055 DAOQPA010000000
VERSION     DAOQPA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_20942_cov_20.425671"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKX01_09310"
     CDS             <1..183
                     /locus_tag="PKX01_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76365.1"
                     /translation="TADLLHAMQALSQLSYTPNQRAAHYKKHCTACKAPPLKIFLKQR
                     NDLRIIDSNNIESKLL"
     gene            complement(343..726)
                     /locus_tag="PKX01_09315"
     CDS             complement(343..726)
                     /locus_tag="PKX01_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HMZ76366.1"
                     /translation="MADKAKEAFKQRIREMTRRSGGRSLPEIVERLRAYLSGWKACFQ
                     LAQTPGVYRELDEWIRHRLRAVQLKHWRRGTTMYRELKAMGASETDARQVAANSRRGW
                     RNSRFLLNRAMPVAYFDRLGVPRLS"
     gene            complement(927..1769)
                     /locus_tag="PKX01_09320"
     CDS             complement(927..1769)
                     /locus_tag="PKX01_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ76367.1"
                     /translation="MSYMAKTKQLAVALGAALTIGGASVAHADVYEYANKFSANYVFS
                     GFSDAGTVGDDHTFTLSFSNVSGNLDATIPASSGYYSISRKGEFDFDYNGTSVPSTLQ
                     APSVCSGLGKANWDFCSKTTNYVPVGAGNFNWSGITGTHFLYNWDTDTFTSNGIPQTT
                     GDFAGNSTAIMLLLGTFLGPVGTTIATYVGDGNVFVHQVMNGAANTWTLTFTEDPGVG
                     IESVLKALDAGTIPGLPKALTNAMIDGVVYADGSLRVVPEPGSLALLGLGAAALAVRR
                     RKSA"
     gene            complement(2022..3425)
                     /locus_tag="PKX01_09325"
     CDS             complement(2022..3425)
                     /locus_tag="PKX01_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ76368.1"
                     /translation="MDAFLDAVRAIVGPTHCLCGEAAAPYLTDWRGRYTGSARAVAFP
                     ADTAEVAALVRVCVDADVPIVPQGGNTGLCGGATPLDDGRALLIQLSRLNRIRHVDGG
                     NNALTAEAGVTLAAVHAAAEAADRQFPLSLASEGSCEVGGVISTNAGGVQVLRYGNTR
                     ELVLGLEVVLPDGQIWNGLRALRKDNTGYDLKHLFIGAEGTLGIVTAATLKLFPRPVA
                     TRTVWLAIPHPMAAVALLGQMRARAGDRLTAFELVSRVSLDLVLQHMPGARDPLPTVH
                     PWYVLAELTDTVRDASLDAMAETLTAEVLESGLVQDGTVASSEAQARALWALREHISE
                     AQKREGYSIKHDISLPVSAIPDFLDDVAGRLEQVWPGLRIVCFGHLGDGNLHYNLSLP
                     VSADNAGLIARSSDVSGLVHDRVHRFGGSISAEHGIGQLKRGLLPRYKSEVELDLMRR
                     IKAAMDPAGRMNPGKVL"
     gene            complement(3436..4353)
                     /locus_tag="PKX01_09330"
     CDS             complement(3436..4353)
                     /locus_tag="PKX01_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ76369.1"
                     /translation="MARRPPRIGIALGSGAARGWAHVGVLRALAREGIEPAVICGSSI
                     GAFVGGIAAAGDLDKLADWVDSLNWQQVVGLLDMGLRGGLIKGDKLIDFFQRHFVDHD
                     FASLRRPFGCVATDLQTGHEVWLRDGSVATAVRASIAMPGLMTPVVHEGRLLVDGGVV
                     NPVPVSLARAMGADLVIAVDLNADVVGRAWRAPNEPPPPPPEAGPFWARKLLTRFGLG
                     ERPAGGAGDDEGIDMPSMLSVIQGSISIMSVRIARSRLAGEPADVLIAPRMAQVGWME
                     FHRGREGIAEGEAAVELALPALRRALESF"
     gene            complement(4366..5697)
                     /gene="rimO"
                     /locus_tag="PKX01_09335"
     CDS             complement(4366..5697)
                     /gene="rimO"
                     /locus_tag="PKX01_09335"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMZ76370.1"
                     /translation="MTQSKQAGVPRVGFVSLGCPKALVDSEHILTRLRAEGYEISPSY
                     DGADLVVINTCGFIDAAVEESLDAIGEALNENGRVIVTGCLGAKEDVVMGAHPQVLAV
                     TGPHATEEVMQAVHKHLPKRHDPFVDLVPPQGIKLTPDHYAYLKISEGCNHRCTFCII
                     PSLRGDLLSRPIGDVMREAENLVNAGVKELLVVSQDTSAYGVDVKYRTGFWGGRPLRT
                     KLYDLCKALGDLGVWVRLHYVYPYPSVDDILPLMAEGKILPYLDVPFQHASPRILKAM
                     KRPASAENNLERVRRWREICPDLTIRSTFITGFPGETDADFEALLQFLEEAQLDRVGA
                     FAYSPVEGAAANELPGAVPEELREERRIQLMEFQEDISTARLERFIGREMTVLVDEID
                     EEGALARSPGDAPEIDGLVTIPAGEHLQVGEFARVRITDCDVHDLYAEPLE"
     gene            complement(5749..6354)
                     /gene="phaR"
                     /locus_tag="PKX01_09340"
     CDS             complement(5749..6354)
                     /gene="phaR"
                     /locus_tag="PKX01_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HMZ76371.1"
                     /translation="MADQVRLIKKYPNRRLYDTQTSSYITLADVKQLVLEHGSFQVVD
                     AKTGEELTRNILLQIILEEEAGGAPMFTSDLLSQMIRFYGNAMQGMMGKYLENNIRAF
                     TDMQAKLQDQVRSVYGDNATVSHDLWAQFLNLQGPAMQSMMAAYMEQSKRMYAQMQEQ
                     LDSQTRNLFSGFQFPSFSGGFAPGKADADEAVAPPPAASKK"
     gene            complement(6463..7203)
                     /gene="phbB"
                     /locus_tag="PKX01_09345"
     CDS             complement(6463..7203)
                     /gene="phbB"
                     /locus_tag="PKX01_09345"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HMZ76372.1"
                     /translation="MTQRIALVTGAVGGLGTAICKSLAAEGIRVVGNFFPGDPAKDAW
                     VEARTAEGLDIPLYGFDVSDYEQCKAAVEKIVAEVGPIDILVNNAGITRDKFFPKMEK
                     GQWDAVIATNLTSLFNVTHTISPKMAERGWGRIVNISSVNGVKGQAGQTNYSAAKAGV
                     IGFTKALAAELATKGVTCNAIAPGYIGTDMVMAIKEEIRQGIIDTVPVKRLGKPEEIG
                     AAVVYLSSELAGYVTGATLNVNGGLYYQ"
     gene            complement(7249..7989)
                     /gene="phbB"
                     /locus_tag="PKX01_09350"
     CDS             complement(7249..7989)
                     /gene="phbB"
                     /locus_tag="PKX01_09350"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HMZ76373.1"
                     /translation="MARVALVTGGMGGLGESICIKLAALGYKVATTHSPGNTKVGEWL
                     QTMNNMGYGFKAYPCDVADFDSCKACIEAVTAELGPVDVLVNNAGITRDMTFKRMTKG
                     DWDAVMSTNLDSVFNMTKQVMDGMVERKWGRVINVSSLNGIKGAFGQTNYAAAKAGMH
                     GFTKSLAQEVAKSGVTINTISPGYIGTKMVLAIPQEVLDSKILPHIPMGRLGKPEEIA
                     GLIAYLSSDEAAFVTGANISINGGQHMF"
     gene            complement(8113..9852)
                     /gene="phaC"
                     /locus_tag="PKX01_09355"
     CDS             complement(8113..9852)
                     /gene="phaC"
                     /locus_tag="PKX01_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HMZ76374.1"
                     /translation="MTPEQATEALQGMFKFGQSLAQNYFDFLGKQSSAMQQAAPVGAP
                     VPAVPLPEAQELARLQQELAESHARLWSTLAQTRPGEKAEAVAHPEPGDRRFSAPEWT
                     SSPIFDYIRQSYLLNSSFLNKVADALPVADGRAKARMQFLTRQYIDALAPSNFAATNP
                     EFIQTALDTHGESITRGIKNLIGDLEKGRISMTDDAAFEIGRNLATTPGSVIYENELM
                     QLIQYAPLTEQVYQRPLLIIPPCINKFYIMDLQPANSLVRFVVEQGFTVFLVSWRNAK
                     AEQAHHTWDDYLEKGPITALQVVREITQVDKPNVLGFCVGGTLLSSSLAVLRGRGEDP
                     AESLTLMTTLLDFGDAGELGCLVDETSVAAREAAIGKGGLLMGRELAQVFSSLRANDL
                     IWQYVVGNYLKGKTPEAFDLLYWNSDSTNLPGPFLAWYLRNMYLENNLRVPGKLEMLG
                     VKVDLGKVDLPAYLLATREDHIVPWTSAYLVRQILGGETTFVLGASGHIAGAINPASK
                     NRRSYWTSESAAAVPEEWLAAATERKGSWWLHWIEWLKTRSGEQIAARVKPGTKKYKV
                     IEAAPGRYVKEKV"
     gene            9983..10207
                     /locus_tag="PKX01_09360"
     CDS             9983..10207
                     /locus_tag="PKX01_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76375.1"
                     /translation="MYIVAIAWLYVALLATAAETTVVGGLLTFFFYGLAPLSLFLWLF
                     GTPARRRRSAQRALDEGMGDGDRGDTGSNQ"
     gene            complement(10139..10930)
                     /locus_tag="PKX01_09365"
     CDS             complement(10139..10930)
                     /locus_tag="PKX01_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMZ76376.1"
                     /translation="MTPLAWIVLMSVGGGALSVLAASVVAYAARESWVPVLISYAIGA
                     LLGAAFLDVLPHAIESAATPTRAAATVLAGILLFFVLEKLVLWRHCHDEGCHGHGHDH
                     HHGHEGHDHGRSGLLILVGDTFHNFVDGVLIAAAFVDSTALGIVTALAIIAHEIPQEV
                     GDFLILLHSGFSRTKALAFNLLSSLATLVGGFLAYFALSQIESLVPVFLALAAASMIY
                     VAVADLIPGLHKRTELRATAQQVSLIAAGIATIAIAHPLIEGALG"
     gene            complement(10970..11716)
                     /gene="pgeF"
                     /locus_tag="PKX01_09370"
     CDS             complement(10970..11716)
                     /gene="pgeF"
                     /locus_tag="PKX01_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HMZ76377.1"
                     /translation="MSNGNWIVPDWPAPACVRALSTTRLGGVSPAPWDSLNLGTHVQD
                     DPARVLANRAILRAQLPADPVWLNQVHGTTVVDAAVVSGIPDADAAIARASGAVCVVM
                     TADCLPVLLCDRAGTVVGAAHAGWRGLHGGVIEATLAAMGVAPHEVLAWLGPAIGPAA
                     FEVGDEVRAAFVADDEAAASAFLPGAAPDKWLADLYRLACQRLARAGVTRVSGGGLCT
                     VADRRRFFSYRRDGLTGRMASLVWLAAPDA"
     gene            complement(11719..12699)
                     /gene="rluD"
                     /locus_tag="PKX01_09375"
     CDS             complement(11719..12699)
                     /gene="rluD"
                     /locus_tag="PKX01_09375"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HMZ76378.1"
                     /translation="MKCAIDIPQEDDWLDYSPADPAPSPQRETVPADCAGLRADQALA
                     RLFPEHSRSRLQAWLKSGRIRIDGASPDAKCKVWGGETVEVSAEPTEESLAQQAEDIP
                     LAVVFEDDTLAVIDKPVGLVVHPGSGNWSGTLLNGLLHHDPAFRALPRAGIVHRLDKD
                     TSGLLVVAKTLEAQTDLVRQLQARSVKRHYYALVHGQPAEQGTVEAPIGRHPVQRTKM
                     AVVRGGREALTRYRVCERFRHATLVECELATGRTHQIRVHMAHIGHPLVGDATYGKAR
                     SADPRLDAFPRQALHAWQLGLIHPRSGEACVWQRALPADFETLLATLRSV"
     gene            12686..13489
                     /locus_tag="PKX01_09380"
     CDS             12686..13489
                     /locus_tag="PKX01_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMZ76379.1"
                     /translation="MAHFTLRSLAFIGVLLLAGCSGLSDQADETAGWSAQKLYTEAKE
                     SMSDGAWDRAIKMFEKLEARYPYGRYAQQSQLEVAYAYYKSGEPASAVAACDRFIKLH
                     PNHPNVDYAYYLKGLAYFNEDLGLLGALANQDMSERDTKASRDAFDTFKDLVTRYPNS
                     VYAADAQARMRYLVNALAAHEVHVARFYLRRGAYVAAANRAQAAVKDFPESPSVEEGL
                     FLMIKAYDAMGLTELRDDADRVLRKNYPNSAFYSGQPRPGEKSAWWKLW"
     gene            13520..14035
                     /locus_tag="PKX01_09385"
     CDS             13520..14035
                     /locus_tag="PKX01_09385"
                     /inference="COORDINATES: protein motif:HMM:NF022609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HMZ76380.1"
                     /translation="MDDIVRQALLKWPDVPDCRGWLHLTRRGAWRVPTGIIHHAGLSA
                     FIGRNYGADETGRWFFQNGPQKVFVSLEYTPYVLRLRSDGSLETHTGLPVDALQAGWL
                     DDDGSLLLDSEHGIGLLDDRDLAAMAEGLEGNPDSAEPLHLRWRDTRVSIGRLKRAEV
                     SARFGFLAEPT"
     gene            complement(14063..15502)
                     /locus_tag="PKX01_09390"
     CDS             complement(14063..15502)
                     /locus_tag="PKX01_09390"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HMZ76381.1"
                     /translation="MHLQPIILSGGSGTRLWPLSREQYPKQLLALVGHETMLQATAGR
                     LAGFAGALPVEQAPLVVCNEEYRFVTAEQLRACGRGGRDIVLEPAGRNTAPALTLAAL
                     AVEEADPVLLVMPADHVIRDLAGFQRAIATGIPAALAGAIVTFGIVPDRPETGYGYIR
                     FGAERNDGARSIAAFVEKPDAATAQRYLDSGEYLWNSGLFMMQASTWLKAMAHLQPAM
                     EAACRAAWAGGSRDADFVRLDKAAFLACPSDSIDYAIMEKLAGAEGLGIPGCVVPMSA
                     GWSDVGAWDALWQIADKDEAGNTARGDVLFENTRNTLVHADSRLVACVGLEDTIVVET
                     PDAVLVAHKERTQDVKQIVARLKADQRSQASAHRKIHRPWGWYDSIDSGERFQVKRIV
                     VNPGARLSLQMHYHRAEHWIVVKGTAEVTNGEKTFLLGENESTYIPLGHVHRLANPGK
                     VPLEIIEVQSGSYTGEDDIVRFEDKYGRG"
     gene            complement(15609..16217)
                     /gene="yihA"
                     /locus_tag="PKX01_09395"
     CDS             complement(15609..16217)
                     /gene="yihA"
                     /locus_tag="PKX01_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMZ76382.1"
                     /translation="MSLFRNARFEISIAKPSGLPPPNGAEVAFAGRSNAGKSSAINTL
                     ANHTRLAFVSKTPGRTQLINFFRLQNGAVLVDLPGYGYAQVPEAVRRQWQHLLENYLT
                     RRPNLIGLVLIMDSRHPLTERDRAMIGWFAPSGRPMHVLLTKSDKLSRNEAAATLAAV
                     RRELAPLGSQVTVQLFSSLKKTGIEEVEQVVGAWLVPAQDPA"
     gene            16350..16985
                     /locus_tag="PKX01_09400"
     CDS             16350..16985
                     /locus_tag="PKX01_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ76383.1"
                     /translation="MIKRSLLLSLLLAATAVQAEEKAPAQPDLAKAKMTAEQVCGACH
                     GADGNSQIPTNPKLAGQHPEYLAKQLKNFKSVDGKPAERANPVMGGMAAPLSDDDITG
                     LAAYFGSQTLKPEAAKNKASIELGQKLWRAGDLKRGIPACAACHGPAGAGLPAQFPRI
                     GGQFADYTEAQLKAFRAGERANDPAKMMRTIALKMTDPEIKAVSDFAAGLR"
     gene            17102..19156
                     /locus_tag="PKX01_09405"
     CDS             17102..19156
                     /locus_tag="PKX01_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HMZ76384.1"
                     /translation="MQRSTLRKTYELLSSMRFAISLLTVLAIASIIGTVIKQNEPYNN
                     YLNQFGPFWFPVFEKLGLYAVYNAAWFLLILGFLILSTGACIVRQTRPMLKDMRSFRE
                     HARETSLAAFQHHALLAVGLPFDEAARFTREHLIRNGFRFRENVRDDGVLFAGRQGSW
                     SRIGYFLAHAAILLICLGGLLDGNLPLKVQMALGGKHITSGNQLIADIGPESRLGRDN
                     WSFRGNVFVPEGKSSNVAVLNVKDGILLQDLPFTVSLRKFTLEHYDSGMPKRFASDVL
                     ITGADGKTYEKTIEVNKPVEVDGIMLYQASFEDGGSRLRLLARDLRVDGTPDQSVDVA
                     VGDSLPLKRNGLDLTLELIAFRPFNVENLADPDTGAASGTLGTLEKHLGSGARSTAPK
                     DLHNVGPSFQYKLRDNTGQAREYQTYMQPIAQDGAWYLLAGMRDTPDAPFRFMRIPVD
                     ADQKADAWFALRRVLLDPRRHDELARRFAATALDRGDDAALRERLQETARRTLDLFAR
                     GGFESVGKFIESTIPEGERDRAAGVFIKVLEGVTWEAWQLARQDAGLPPLKNDAQYAR
                     FVRDSLNAASDSQHYGAPLLFQLDRFDEVRATVLQATRSPGKPIVYLGSLLLVLGVFA
                     MLYIRERRLFVLVKHSGEALVALSSNRKSLDVDTAFAAHRDALATRFTPPADAPPAIE
                     PR"
     gene            19162..20301
                     /gene="ccsB"
                     /locus_tag="PKX01_09410"
     CDS             19162..20301
                     /gene="ccsB"
                     /locus_tag="PKX01_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HMZ76385.1"
                     /translation="MDIAHPDTPSLLRRLQPGDWLYALAVAGGGMFALRQYGGFMDIY
                     DKAILALVVPAVVAAGWHWKPFRLFLAAVAALALTSVGLYEGDLARAEKIFLLKYFLS
                     SQTAIMWMSALVFLATGSYGLGLAARSDFAEKTGSALTWTSATFGTTGLFVRWYESYL
                     IGRDVGHIPLSNLYEVFVLFTLLTSLLYLYYEGRYATRRMGAFVLLVISAAVSFLLWY
                     TFSREAQEIQPLIPALQSYWMKVHVPANFIGYGAFALAAMIGVADLLVRRGLLASRLP
                     APEVLDDVMYKAIAIGFAFFTIATILGALWAAEAWGTYWQWDPKETWAFIVWLNYAAW
                     LHIRLTKGLRGALLAWWAVVGLLVTAFAFVGVNMFLSGLHSYGSL"
     gene            complement(20286..>20942)
                     /locus_tag="PKX01_09415"
     CDS             complement(20286..>20942)
                     /locus_tag="PKX01_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HMZ76386.1"
                     /translation="LLPHTDLPRRKPTTPEGHAALLHAIAHIEFNAINLALDCVYRFR
                     SLPDEFYAGWLQVAAEEADHFARVCTRLAALGHAYGDFPAHRGLWDMAVKTAEDPLAR
                     MALVPRVLEARGLDATPPIVAALHRIGDADSIAVLDIILRDEVGHVALGDRWFRHLCA
                     ERGLEPEATYLALMADFDAPWPPGPLNVEARRRAGFSAAEIVRLEAGRPRGKVPQSEP
                     "
BASE COUNT         3713 a         6916 c         6663 g         3650 t
ORIGIN      
        1 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatac ccccaatcaa
       61 cgagctgcgc attataagaa acattgcaca gcctgtaaag caccgccgct caaaatattt
      121 ttgaagcaac gaaacgattt gagaattata gattcgaata atattgaaag caagcttcta
      181 tgaaaaattt tttgtggaag ccgtccgcct ctcatgcgta cctgcctgaa gcccttctct
      241 gcggcgcggc caatcgggat aggggcggtc agatcacccg accgttcccc tcccacacca
      301 cccggcatgc gggtccgcac cgggcggttc gagcagttga ggtcatgaga gtcgaggtac
      361 accgagccga tcaaagtagg cgacaggcat ggcacggttc agtaggaagc ggctgttacg
      421 ccaccccctc cgactgttcg cagccacctg ccgagcgtcc gtttccgagg cgcccatcgc
      481 cttcaattcc cgatacatcg tcgtgccccg acgccagtgc ttgagctgca ccgcacgcag
      541 tcgatggcgg atccactcgt cgagttcgcg atacaccccg ggcgtctgtg caagctggaa
      601 gcacgccttc cagcccgaca ggtaagcccg cagtctttcg acgatctctg gcaggctgcg
      661 cccgcccgag cgtctcgtca tctcccggat gcgttgcttg aaggcctctt tggctttgtc
      721 ggccaccttg caccgtacct gtccgcctga cccgtaccag aatccacgcg tcctccccac
      781 gctcggtccg ccctcacgca gttgcgcttc tcttcgttcg ctgtgatcaa cttacggcgg
      841 gacttgcacc cacaggagtg cccccatgta gggcgcacaa aacaaaaacg gcggccgaag
      901 ccgccgtttt gccgagacag aaccgctcag gcgctcttgc ggcgacgcac tgccagtgca
      961 gccgcaccca gacccagcag cgccaggctg cccggctccg gaacgacgcg cagactaccg
     1021 tcggcgtaca cgacgccatc gatcatggcg ttcgtcagcg ccttcgggag gcccggaatg
     1081 gtgccggcat ccagcgcctt tagcacggat tcgatgccaa cgccgggatc ttcggtgaaa
     1141 gtcagcgtcc aggtattcgc agcgccgttc atgacctgat gcacgaacac gttgccatcg
     1201 cccacatagg tggcgatggt cgtgccgacg gggccgagga aggtgcccag cagcagcatg
     1261 atggccgtgg agttgccggc gaagtcaccc gtggtctggg gaatgccgtt ggacgtgaat
     1321 gtgtcggtgt cccagttgta gaggaaatgg gttccagtga tcccggacca gttgaagttg
     1381 cccgcaccga ccggcacgta gttagtggtc ttggagcaga aatcccagtt ggccttgccc
     1441 aaaccgctac acacggaagg tgcctgaagg gtggacggaa cagaggtgcc gttatagtcg
     1501 aagtcgaact cgcccttgcg ggaaatggag tagtaaccgg acgaggcggg gatggtggca
     1561 tccaggttgc cgctgacgtt gctgaacgac agggtaaagg tgtgatcatc gcccacagtg
     1621 cccgcgtcgg agaaacccga gaacacgtag ttcgcgctga acttgttggc atactcatag
     1681 acatcagcgt gggccacgga ggcgccgccg atggtcaggg ccgcgcccag ggcgacggca
     1741 agctgttttg ttttggccat gtaactcatc cttatttcaa gttgaaccga atccaattac
     1801 cacccgggaa ggccgtgaag cgcttcacgc agtactctac acacagatta aaagcaagca
     1861 tcacgccagg tcagataagc ccatgttatc cattgctaaa atgaactgcg aagctggcag
     1921 atctgtcagg aatgtaaaat tctccgacac agacccctcc cgccgtcccg tgagatcgtt
     1981 cacaccgcga ttccccacag gaagcgctgg ccacggtgac ctcacagcac tttccccgga
     2041 ttcatccgcc cggctgggtc catggcagcc ttgatgcggc gcatcagatc cagttccacc
     2101 tctgatttgt agcggggcag caggcctcgc ttgagctgac cgatgccatg ctcagccgaa
     2161 atggatccac cgaatcgatg cacccgatca tggacgaggc cactgacgtc cgaactccgg
     2221 gcaatcagcc cggcattgtc cgccgacaca ggcagagaca ggttgtaatg cagatttccg
     2281 tctcccaggt gcccgaagca cacgatgcgc aggcccggcc acacctgttc gaggcgaccg
     2341 gccacgtcat cgagaaagtc ggggatggcg ctcaccggca atgaaatatc gtgcttgatg
     2401 ctgtagccct cacgcttctg ggcttccgaa atgtgttcgc gtagcgccca cagtgcgcgc
     2461 gcctgtgctt cgctgctggc gaccgtacca tcctgcacga ggcccgattc cagcacctcg
     2521 gccgtcagcg tttccgccat ggcatccaga gaggcgtccc ggacggtgtc ggtcagttcc
     2581 gccagcacgt accaggggtg aaccgtcggc aagggatccc gcgcgccggg catgtgctgc
     2641 agcaccagat cgagactcac gcggctcacc agctcgaagg ccgtcagccg gtcaccggca
     2701 cgggcgcgca tctgcccgag caaggccacg gcggccatcg gatggggaat cgcaagccac
     2761 accgtccggg ttgcgaccgg gcgaggaaac agcttcaggg tggcggccgt gacaatgccc
     2821 agcgtgcctt cggcgccgat gaacaagtgc ttcagatcgt agccggtatt gtccttgcgc
     2881 agtgcgcgca ggccgttcca gatctggccg tcgggcagga ccacttccag gcccaggacc
     2941 agttcccggg tgttcccgta acgcagcacc tgcaccccgc cggcattggt ggaaatcacg
     3001 ccgcccacct cgcaggatcc ctcgctggcc aaggacaggg gaaattgccg atccgccgct
     3061 tcggcggccg catgtacggc tgcgagcgtc acccctgcct cagcggtgag ggcattgttg
     3121 ccgccatcca cgtggcgaat gcgattcagg cgcgacagtt ggatgagcag cgcacgcccg
     3181 tcatccaggg gagtcgcgcc gccgcacagg ccggtgttgc cgccctgggg cacgatgggc
     3241 acgtcggcat ccacgcagac gcgcaccagc gcagcgactt cggcagtatc ggccggaaag
     3301 gccaccgcgc gtgcggagcc ggtataacgg ccacgccagt cggtcagata cggagcggcc
     3361 gcttcgccgc acaggcagtg ggtcggaccg acaatggccc ggaccgcatc gagaaaggca
     3421 tccatgaagt ggatgctaga aggactccag ggcgcgacgc aaggccggca gcgccagttc
     3481 gacggcggct tcgccctcgg cgatgccttc ccgaccgcga tggaactcca tccagcctac
     3541 ctgggccatg cgcggtgcga tgagcacgtc ggccggttcg cccgccagcc ggctgcgggc
     3601 aatgcgcacg gacatgatgg agatgctgcc ctggatgacc gacagcatgg acggcatgtc
     3661 gataccctcg tcgtcgccgg cgccgcccgc aggtcgctcg cccaggccaa agcgggtcag
     3721 cagcttgcgc gcccagaacg gcccggcttc tggcgggggc ggaggcggtt cgtttggcgc
     3781 acgccaggcg cgaccgacca catcggcgtt gagatcgacg gcaatgacga gatcggcgcc
     3841 catggcacgg gcgagggaca ccggcacggg attgacgacg ccgccatcca ccagcagccg
     3901 gccttcgtgt acgacgggcg tcatcaggcc aggcatggcg atggaggcgc gcacggcggt
     3961 cgccacgctg ccgtcccgca accacacttc gtgccccgtc tgcagatccg tggcgacaca
     4021 gccgaaaggg cgccgcagcg aagcgaagtc gtggtcaaca aagtggcgct ggaagaagtc
     4081 gatgagcttg tcgcccttga tgagcccgcc ccgcagaccc atgtcgagca gtccgaccac
     4141 ctgctgccag ttgagactgt ccacccagtc ggcaagcttg tccagatcgc ccgccgcggc
     4201 gattccgccg acaaaggcac cgatggaact gccacagatc accgcgggct cgatgccttc
     4261 ccgcgccagg gcgcgcagta cgcccacgtg ggcccagccc ctcgccgcgc cgctgccgag
     4321 ggcaatgccg atgcggggcg ggcggcgggc catggagccg ctcggctact cgaggggttc
     4381 ggcgtagagg tcgtgcacgt cgcagtcggt gatgcgcacg cgggcgaact cgccgacctg
     4441 caggtgctcg cccgccggga tcgtcaccag gccgtcgatc tccggtgcgt cgccgggcga
     4501 tcgggccagg gcgccctcct cgtcgatctc gtccaccagc acggtcatct cgcggccgat
     4561 gaagcgctcg aggcgcgccg tggagatgtc ctcctggaat tccatgagct ggatgcgccg
     4621 ctcttcgcgg agttcttccg gcacggcgcc gggcagctcg ttggcggccg cgccttccac
     4681 cggtgaatac gcaaaggcac cgacgcggtc gagctgcgct tcttcgagga actggagcag
     4741 cgcttcgaaa tcggcgtcgg tctcgccggg aaagccggtg atgaaggtgg atcggatcgt
     4801 gaggtccggg cagatctcac gccagcggcg gacccgctcc agattgttct ctgcactggc
     4861 gggccgcttc atggccttga ggatgcgcgg gctggcatgc tggaagggca catccaggta
     4921 gggcaggatc ttgccttcgg ccatcagcgg cagaatgtca tccacactcg ggtaaggata
     4981 gacataatgc agacgcaccc acacgcccag atcaccgagc gccttgcaca ggtcgtagag
     5041 cttggtacgc agcggccgcc caccccagaa accagtgcgg tacttcacgt ccacgccata
     5101 ggcgctggtg tcctgggaca ccaccagaag ctccttgacg ccggcgttga ccaggttctc
     5161 ggcctcgcgc atcacgtcgc cgatgggccg gctgagcaga tcgccgcgca gggacgggat
     5221 gatgcagaag gtgcagcgat gattgcagcc ttcggaaatc ttcaggtagg cgtaatgatc
     5281 gggcgtcagc ttgataccct gaggcggcac cagatcgacg aagggatcgt gtcgtttggg
     5341 caggtgcttg tgcaccgcct gcatgacctc ttcggtggcg tggggcccgg tgacggccag
     5401 cacctggggg tgtgcgccca tcaccacgtc ttccttggcg ccaagacagc cggtgacgat
     5461 gacccggccg ttctcgttga gggcttcgcc gatggcgtcg agggattcct ccaccgccgc
     5521 atcgatgaag ccgcaggtat tgatgaccac cagatcggcg ccatcgtagg acggtgaaat
     5581 ctcgtagcct tcggcgcgca ggcgggtgag gatgtgctca gagtccacca gggccttggg
     5641 gcacccgaga gacacgaagc cgacgcgggg aactcccgct tgcttggatt gggtcatgcg
     5701 tgcgaatggg aaaaggcacc ggccgcagcg caggctgcgg cccggagatc atttcttgga
     5761 cgcggcgggt ggcggcgcaa cagcctcgtc ggcgtcggcc ttgcccgggg caaagccgcc
     5821 ggagaaactg gggaactgga aaccagagaa cagattacgg gtctggctgt ccagttgctc
     5881 ctgcatctgg gcatacatcc gcttgctctg ctccatgtag gcagccatca tactctgcat
     5941 ggcggggccc tggaggttga gaaactgggc ccacaggtca tggctgacgg tggcgttgtc
     6001 gccatacacc gagcgaacct gatcctgcag cttggcctgc atgtcggtaa aggcccggat
     6061 gttgttttcc aggtacttgc ccatcatccc ctgcatggca ttcccgtaga agcggatcat
     6121 ctgcgaaagc aggtcgctgg tgaacatcgg cgcaccgccc gcttcctctt cgaggatgat
     6181 ctgcaaaagg atgttgcggg ttagttcctc gccggtcttt gcatcgacca cctggaagga
     6241 gccatgctcg agcacgagct gcttcacgtc ggcgagcgtg atgtaggaac tggtctgggt
     6301 atcgtaaagg cggcggttcg ggtatttctt gatcaggcgg acttgatcgg ccatgtattt
     6361 atctcctcac tggcgatcat gtcgcctgtg atgattatac tgcgctgcgc aaaaaaaagc
     6421 cccgccggac tggcggggct cagtgacgcg tttcgcggca gcttattgat aatacaggcc
     6481 gccgttgacg ttcagggtgg cgccggtgac gtagccagcc agttcggagg acagataaac
     6541 caccgcagcg ccgatctctt ccggcttgcc cagacgcttg acgggcacgg tgtcgatgat
     6601 gccctgacgg atctcttcct tgatcgccat gaccatgtcg gtaccgatgt agcccggagc
     6661 gatggcgtta cacgtcacgc ccttggtggc cagctcggcc gccagcgcct tggtgaagcc
     6721 gatcacgccc gccttggcgg cggagtagtt ggtctgaccg gcctgaccct tgacgccgtt
     6781 caccgaggag atgttcacga tgcggcccca gccacgctcc gccatcttgg gggagatggt
     6841 gtgggtcacg ttgaacaggc tggtgaggtt ggtcgcaatg accgcatccc actggccctt
     6901 ttccatcttc gggaagaact tgtcgcgggt gatgccggcg ttgttcacca ggatgtcgat
     6961 cgggccgact tcggcaacga tcttctcaac ggccgccttg cactgctcgt agtcagacac
     7021 gtcgaaaccg tacaggggga tgtccagacc ttcagccgtg cgggcttcga cccaggcgtc
     7081 tttggcggga tcaccgggga agaagttgcc gacgacacgg atgccttcag cagcgaggga
     7141 cttgcagatc gcggtgccga ggccaccgac cgcgccagta acaagagcga tgcgttgagt
     7201 caattcactt tccttttatt gcagttgcag tgccaaagaa agcggccatt agaacatatg
     7261 ctgaccgccg ttgatagaaa tgttggcacc ggtcacgaac gcagcctcgt cggaggagag
     7321 ataggcgatc agaccggcga tttcttccgg cttaccgagg cgacccatcg gaatgtgcgg
     7381 caggatcttg ctgtccagca cttcctgggg aatcgccagc accatcttgg tgccgatgta
     7441 acccggagaa atggtgttga tggtgacccc ggacttggcc acttcctgcg ccagcgactt
     7501 ggtgaagccg tgcatgccgg ccttcgctgc ggcgtagttg gtctgtccga aagcaccctt
     7561 gatgccattc agggacgaaa cgttgatgac gcggccccac ttgcgttcga ccatgccatc
     7621 catgacctgc ttggtcatgt tgaaaacgct gtccaggttg gtgctcatga ccgcatccca
     7681 gtcgcccttg gtcatgcgct tgaaggtcat gtcgcgggtg atgccggcgt tgttcaccag
     7741 cacatccaca ggccccagct ccgcggtgac ggcctcgatg caggccttgc aggagtcgaa
     7801 gtctgcaacg tcacagggat aggccttgaa accgtagccc atgttgttca tggtctgcag
     7861 ccattcgccc accttggtgt tgcccgggga atgggtggtg gccaccttgt acccgagcgc
     7921 tgccagcttg atgcagatgg actcgccgag tccgcccata ccacccgtta ccagtgctac
     7981 acgtgccatg tttctccctc cttatatggt tttgcagggg tcagttacca ccggcggcgc
     8041 ccccttcacg ctgcgccagt gtgcggtccg tccgtcccgt attcgatgcc aggcatcgaa
     8101 caacgaacag gctcagacct tttccttcac atagcggccg ggcgcggctt cgatgacctt
     8161 gtattttttg gtgcccggct tgacccgtgc agcgatctgc tcgccactgc gggtcttgag
     8221 ccattcgatc cagtgcagcc accagctgcc cttgcgctcg gtggcagccg ccagccattc
     8281 ttccggcacg gcggcagccg actcactggt ccagtagctg cgacgattct tggaggccgg
     8341 attgatggcc cccgcaatgt gcccgctcgc ccccagcacg aaggtggttt cgccgccgag
     8401 gatctgccgg accagatagg cgctggtcca cggcacgata tggtcctccc gcgtggcgag
     8461 caggtaggcg ggcaaatcca ccttacccag gtccaccttg acgccgagca tctccagctt
     8521 gcccggcacg cgcagattgt tttccaggta catgttgcgc aggtaccagg caaggaaggg
     8581 ccccggcagg ttggtactgt cggagttcca gtaaagcagg tcgaaggcct cgggcgtctt
     8641 gcccttgagg taattgccga ccacgtactg ccagatcagg tcgttggcgc gcaggctgga
     8701 gaacacctgg gccagttcgc gacccatgag cagtccgccc ttgccgatcg ccgcctcgcg
     8761 ggccgccacg gaggtctcgt ccaccaggca gcccagttcg cccgcgtcgc cgaaatccag
     8821 cagcgtggtc atgagggtca ggctctcggc cgggtcttct ccgcgtccgc gcaggaccgc
     8881 cagggacgac gacagcagcg taccgccgac gcagaatccc agcacgttgg gcttgtcgac
     8941 ctgggtgatc tcgcgcacca cctggagggc ggtaatcggc cctttctcga ggtaatcatc
     9001 ccaggtgtga tgcgcctgct cggccttggc gttcctccag gacacgagaa agacggtgaa
     9061 gccctgttca acgacgaacc gcaccagcga attcgccggt tgcaggtcca tgatgtagaa
     9121 cttgttgatg cagggcggga tgatcaacag ggggcgctgg taaacctgct ccgtcagcgg
     9181 cgcgtactgg atgagctgca tcagctcgtt ttcgtagatc accgagccgg gcgtcgtggc
     9241 caggttgcgg ccgatttcga aggcggcgtc atcggtcatc gagatgcggc ccttttccag
     9301 gtcgccaatg agattcttga tgccgcgggt gatgctctcg ccgtgggtat cgagggcggt
     9361 ctggatgaac tccggattgg tcgcagcgaa gttggacggc gccagggcgt cgatgtactg
     9421 acgcgtcagg aactgcatgc gtgccttggc ccgaccgtcc gccaccggca gcgcatcggc
     9481 caccttgttc aggaaggagg agttgagcag gtaggactga cggatatagt cgaagatcgg
     9541 actggaggtc cattccggtg cgctgaagcg acgatcgccc ggttcgggat gggccacggc
     9601 ttcggctttc tcacccggac gggtctgcgc cagcgtgctc cagaggcggg cgtgggattc
     9661 ggcgagttcc tgctggagtc gcgccagttc ctgcgcttca ggcagcggaa cggccggcac
     9721 gggtgctccc accggagccg cctgctgcat ggcggagctc tgcttgccaa ggaaatcgaa
     9781 ataattctgc gccaaggact ggccgaactt gaacatgccc tgcaatgcct ctgttgcctg
     9841 ctccggtgtc atgcacctct cctgatgttt ttatccttgc ggcccggctc ccgacccaac
     9901 gccgcatcgc ccgggagcgt cgtgtcgccc ccgcggacgc acgaaccccc gcgagacgta
     9961 cgcggccaag tagaatccgg gtatgtatat cgttgcgatt gcctggctgt atgtcgcgct
    10021 gcttgccact gcagcggaga ccaccgtcgt cggaggtctc ctgacgttct tcttctatgg
    10081 tcttgcgccg ctctccctgt tcttgtggct cttcggcaca ccagcccgcc gtcgccgttc
    10141 agcccagcgc gccctcgatg aggggatggg cgatggcgat cgtggcgata ccggcagcaa
    10201 tcaatgatac ctgctgggcg gtggcgcgca attccgtcct tttgtgcaag ccgggaatga
    10261 ggtcggccac cgcaacgtag atcatactcg ccgccgcgag cgcgagaaat accggaacca
    10321 gcgattcgat ctgcgacagg gcgaaatacg ccaggaagcc ccccaccagc gtcgccagac
    10381 tcgacagcag attgaaggcc agcgccttgg tccggctgaa gcccgagtgg agcaggatca
    10441 ggaaatcccc cacttcctgc gggatttcat gggcgatgat cgcgagcgcg gtcacgatgc
    10501 ccagggccgt gctgtcgaca aatgccgcgg caatcagcac gccgtctaca aagttgtgga
    10561 aggtgtcgcc gacgaggatc agcagcccac tgcgaccgtg atcatgacct tcgtgcccat
    10621 gatggtggtc atgcccatgg ccatgacacc cctcgtcatg gcagtggcgc cacaggacca
    10681 gtttttccag cacaaagaaa agcaggatgc ccgccaggac cgtcgccgcc gcccgtgtag
    10741 gcgtggcggc cgactcgatg gcatggggca gcacgtcgag aaaagccgcg ccgagcagcg
    10801 cgccgatggc gtagctgatc agcaccggaa cccaggactc ccgcgccgca taggccacga
    10861 cggacgcagc cagaacgctc agggcgccgc cgccgacact catcaggaca atccaggcca
    10921 ggggcgtcat ggaattcgga tacacaaaaa ggccggcgag tatataccct caggcgtccg
    10981 gcgcggccag ccacaccagc gaggccatac gtccggtgag gccgtcccgg cgataggaga
    11041 agaaccgccg gcgatccgcc acggtacaca gcccgccgcc ggacacacgc gtgacgcctg
    11101 cccgcgccag acgttgacac gccagccgat agagatccgc cagccacttg tcgggcgcag
    11161 ctcccggcag aaaagcggac gccgccgcct catcgtcggc gacgaaggcc gcccgcacct
    11221 cgtcgcccac ctcgaaggcc gcagggccga tggccgggcc gagccaggcg agcacctcgt
    11281 gtggcgccac gcccatggcg gccagcgttg cctcgatcac cccgccgtgc agcccgcgcc
    11341 agcccgcatg ggccgcgccg acgaccgtcc cggcccggtc gcagagcaaa acgggcagac
    11401 agtcggcagt catgacgacg cacacggcgc ccgacgcgcg ggcgatcgcg gcatcggcgt
    11461 ccggaatgcc agacacgacc gccgcatcca ccacggtcgt gccatgcacc tgattgagcc
    11521 agaccgggtc ggccggcagc tgcgcacgga ggatcgcccg attggcaagc acgcgggcgg
    11581 gatcatcctg cacgtgggtg ccgagattca gactgtccca gggcgcaggg ctcacgcccc
    11641 ccaggcgggt ggtggacagg gcccggacgc aggccggggc aggccagtcc ggaacgatcc
    11701 agttgccgtt ggacatcctt agacgctccg caaggtggcc agcagggttt cgaaatccgc
    11761 tggcagggcg cgctgccaga cacaggcctc accactgcgg ggatggatca ggcccagctg
    11821 ccaggcatgc agggcctggc gcggaaaggc atcgagtcgc ggatcggcgc tgcgggcctt
    11881 gccgtaggtg gcatccccca ccagtgggtg gccgatgtgg gccatatgaa cacggatctg
    11941 gtgggtgcgc ccggtggcaa gttcgcattc caccagcgtc gcgtgacgaa agcgttcgca
    12001 cacacgataa cgcgtgaggg cttcgcggcc accgcgcacg accgccatct tggttcgctg
    12061 cacgggatga cgaccgatgg gcgcctccac cgtaccctgc tccgcgggtt ggccgtgtac
    12121 gagggcatag taatggcgct tgacgctgcg ggcctggagc tggcggacca ggtcggtctg
    12181 ggcctccagc gttttggcca ccaccagcag gccgctggtg tccttgtcga gacgatggac
    12241 gatgcccgcc cggggcaggg cgcggaatgc cggatcgtga tgcagcagcc cgttgagcaa
    12301 ggtgccggac cagttgccgc tgcccggatg caccaccagt ccgacaggct tgtcgatgac
    12361 ggcgagggtg tcatcctcaa aaaccaccgc aagggggatg tcctcggcct gctgggccag
    12421 gctttcctcg gtgggttcgg cgctcacctc caccgtctcg ccgccccaca ccttgcactt
    12481 ggcgtcgggt gaggcgccgt cgatgcggat tcggccggac ttcagccagg cctgcagacg
    12541 gctgcgggaa tgctcgggaa acaggcgcgc caaagcttga tcggcgcgca ggccggcaca
    12601 gtcggcggga acggtttcgc gttgcggaga aggggcggga tcggctgggc tataatcgag
    12661 ccagtcatct tcctgcggaa tatcaatggc gcacttcacc ctgcgtagtt tagcgtttat
    12721 cggcgttctc ctgctggccg gctgcagcgg cctgtccgac caggcagacg aaaccgctgg
    12781 ctggagcgcc cagaaactct acaccgaagc caaggaatcc atgtccgacg gggcctggga
    12841 ccgggccatc aagatgttcg agaagctgga ggcgcgctac ccctatggcc gctacgccca
    12901 gcagtcccag cttgaggtgg cctacgccta ttacaagtcc ggcgagccgg cctccgcggt
    12961 ggccgcctgc gaccgcttca tcaagctgca cccgaaccat cccaatgtgg attacgccta
    13021 ctacctcaag ggcctggcgt acttcaacga ggatctgggc ctgctcggcg ccctcgccaa
    13081 ccaggacatg tcggaacggg acaccaaggc ctcccgcgac gccttcgaca ccttcaagga
    13141 tctggtgacc cgctacccca acagcgtcta tgccgccgac gcccaggcgc gcatgcgcta
    13201 cctcgtcaat gccctggccg cccacgaggt gcatgtcgcc cgtttctacc tgcgacgtgg
    13261 cgcctacgtg gcggcggcca accgggcgca ggctgcggtg aaggactttc cggaatcccc
    13321 ctcggtggag gaaggcctgt tcctgatgat caaggcctat gacgcgatgg gactgaccga
    13381 gctgcgcgac gacgccgacc gggtcctgcg caagaactat ccgaacagcg ccttctattc
    13441 cggacagcca cggccgggcg aaaagagcgc ctggtggaag ctctggtaag tccccgccct
    13501 cgccggcacc gcgcccacca tggatgacat cgtccgtcag gcccttctga agtggccaga
    13561 cgtgcctgac tgccgaggat ggctgcacct gacccggcgg ggcgcctggc gcgttccgac
    13621 cggcatcatc caccacgccg gactgagcgc cttcatcggc cgcaattacg gcgcggatga
    13681 aaccggccgc tggtttttcc agaacgggcc gcaaaaggtc tttgtcagcc tggaatacac
    13741 gccttacgtc cttcgcctgc gatccgacgg cagcctggaa acccataccg gactgcccgt
    13801 cgatgcgctt caggcgggct ggctggacga cgacggcagt ctcctgctcg acagcgagca
    13861 cggcatcggt ctgctggacg atcgggatct ggctgcaatg gcggagggcc tggaagggaa
    13921 tccggactcc gccgagcccc tgcacctccg ttggcgcgac acgcgggtga gcatcgggcg
    13981 actgaagcgg gccgaggtgt cggcccgctt cggcttcctg gcggaaccga cctgacgccg
    14041 gtccgcgtcg tcattgctga gttcagccgc gcccgtactt atcctcaaac cgcacgatgt
    14101 cgtcctcgcc ggtgtaactg ccggactgca cctcgatgat ctccagcggc accttgcccg
    14161 gattggccag acgatgcacg tgccccaggg ggatgtaggt cgattcgttc tcccccagca
    14221 ggaaggtctt ctcgccgttg gtcacctcgg cagtgccctt cacgacgatc cagtgctcgg
    14281 cccggtggta gtgcatctgc agcgacaggc gcgcgccggg attgaccacg atgcgcttga
    14341 cctggaagcg ctcgccgctg tcgatggagt cgtaccagcc ccacggacgg tggatcttgc
    14401 ggtgggcaga ggcctggctg cgctggtcgg ccttgagacg ggcgacgatc tgcttgacgt
    14461 cctgggtgcg ttccttgtgg gccaccagca cggcgtcggg ggtttcgacg acgatggtgt
    14521 cttccagccc gacgcaggcc accaggcggc tgtcggcgtg gacgagggtg ttgcgggtgt
    14581 tttcaaagag gacgtcgccc cgcgcggtgt tgccggcttc gtccttgtcg gcgatctgcc
    14641 acagggcgtc ccaggcgccc acgtccgacc agccggcact catggggacg acacagcccg
    14701 ggatgccgag cccctcggcg ccagcgagct tttccatgat ggcgtagtcg atggagtccg
    14761 acgggcaggc gaggaaggcg gctttatcca ggcgcacgaa gtcggcgtcc cgcgaaccgc
    14821 ccgcccaggc ggcgcggcag gcggcttcca tggccggctg caggtgggcc atggctttga
    14881 gccaggtgct cgcctgcatc atgaagaggc cgctgttcca caggtattcg ccgctgtcca
    14941 ggtagcgctg ggcggtggcg gcgtcgggct tttcgacgaa ggcggcaatg ctgcgggcgc
    15001 catcgttgcg ttcggcgccg aagcggatgt agccgtagcc ggtttcggga cggtccggaa
    15061 cgatgccgaa ggtgacgatg gcgccagcga gggcggccgg gatgccggtg gcgatcgccc
    15121 gctggaagcc ggccagatcg cggatgacgt ggtcggcggg catcaccagc aggaccgggt
    15181 cggcctcttc cacggcaagg gcggcgagag tcagggccgg ggcagtgttg cgtccggccg
    15241 gttcgaggac gatgtcgcgg ccgccccgcc cgcaggcgcg cagttgttca gcggtgacga
    15301 atcggtattc ctcgttacac acgacgaggg gtgcctgttc gaccggcagc gcgccggcaa
    15361 agccggccag ccggcccgcg gtggcctgga gcatggtctc gtgccccacc agggcgagga
    15421 gctgcttggg gtattgctcg cgcgacaggg gccacaggcg ggtgccggag ccgccggaga
    15481 ggatgatcgg ttgcaggtgc atggaggacg tcatgattgg acgaacggga gggctcttac
    15541 gagcgatgcg cgatgatctc acccggtcgt cgtgacgccg agtgaaaaat gttgctcgtc
    15601 atgaaagttc aggcgggatc ctgcgccggc accagccacg cccccaccac ctgctccacc
    15661 tcctcgatgc ccgtcttctt gaggctggag aagagctgca ccgtgacctg gctgcccagg
    15721 ggcgcaagtt cgcgccgcac cgccgccagc gtggcagcag cctcgttgcg ggaaagcttg
    15781 tcggatttgg tcaggagcac gtgcatcgga cggccgctcg gggcgaacca gccgatcatc
    15841 gcccggtccc gctcggtgag gggatgacgg gaatccatga tcagcaccag tccgatcagg
    15901 ttcgggcggc gcgtgaggta gttttccagc agatgctgcc actggcggcg gactgcctcc
    15961 ggtacctggg cgtagccgta gcccggcaga tccaccagaa ctgcgccatt ctgcaaccgg
    16021 aagaagttga tcagttgcgt gcggccgggc gtcttggaca cgaaagccag ccgggtatga
    16081 ttggccagcg tattgatggc acttgacttg cccgcgttgg aacgcccggc aaaggcaacc
    16141 tccgcaccgt tgggcggggg caggccgctg ggcttggcaa tggaaatctc gaagcgggcg
    16201 ttgcggaaca gggacatgag gtgaggcggg cccatcgcgg gcgctcgcgg cagacggcgc
    16261 ggcgcacaac gcaggcatcg cgcaaataac ggaagttgct atagaataac aggtttgaga
    16321 caccaccatt cttggcttcc gaggacatca tgatcaagcg ttccctgctg ctttcgctgc
    16381 ttctggccgc cacggcggtc caggctgaag agaaggctcc cgcccagccc gacctggcca
    16441 aggccaaaat gaccgccgaa caggtctgcg gcgcctgcca cggtgccgac ggcaacagcc
    16501 agattcccac caatccgaag ctggcagggc aacatccgga atacctcgcc aaacagctca
    16561 agaacttcaa gagtgtggac ggcaagcccg ccgaacgcgc caacccggtc atgggcggca
    16621 tggcggcgcc cctgtccgac gacgacatca ccggcctcgc cgcctacttc ggcagccaga
    16681 ccctcaagcc cgaggcggcc aagaacaagg ccagcatcga gctcggtcag aagctctggc
    16741 gtgccggcga cctgaagcgc ggcatccccg cctgtgcagc ctgccacggc cccgccggtg
    16801 ccggcctgcc cgcccagttc ccgcgtatcg gcggtcagtt tgccgattac accgaagccc
    16861 agctgaaggc cttccgtgca ggtgagcgtg ccaacgatcc ggccaagatg atgcgcacca
    16921 ttgccctcaa gatgaccgat ccggaaatca aggccgtttc cgacttcgcc gccggcttgc
    16981 gctgaatcaa cgcaatcccg acgcgccgac gctaaaaagg ggtggtttcg accacccctt
    17041 tttcattcgg cacgctcgtc ggccgtcaca ctgctctccg cctccatcgc ccaccccagc
    17101 catgcagcgc agcacccttc gcaagactta cgaactcctg agctcgatgc gctttgcgat
    17161 cagcctgctg acggtcctgg ccatcgcctc gatcatcggc acggtgatca agcagaacga
    17221 gccctataac aattacctca accagttcgg accgttctgg ttcccggtat ttgaaaagct
    17281 cggactctac gcggtctaca acgccgcctg gttcctgctc atcctcggtt tcctgatcct
    17341 gtccaccggc gcctgcatcg tccgccagac ccgcccgatg ctcaaggaca tgcgcagctt
    17401 ccgcgagcat gcgcgggaaa cctccctggc cgccttccag catcatgccc ttctggccgt
    17461 cggcctcccc ttcgacgagg cggcccgctt tacccgtgag cacctcatcc gcaatggctt
    17521 ccgcttccgc gagaacgtcc gtgacgacgg cgtcctgttc gccggccggc aggggagctg
    17581 gagccgcatc ggctacttcc tggcccatgc ggccatcctc ctcatctgcc tgggcggcct
    17641 gctggacggc aacctgcccc tcaaggtcca gatggccctg ggtggcaagc acatcaccag
    17701 cggtaaccag ctgatcgccg acatcgggcc cgagagtcgc ctgggccggg acaactggag
    17761 cttccgcggc aacgtcttcg tccccgaagg caagagcagc aacgtcgcgg tgctcaacgt
    17821 gaaggacggc atcctgctcc aggacctgcc cttcaccgtg tccctgcgca agttcacgct
    17881 ggaacactac gattccggga tgcccaagcg cttcgccagc gacgtcctca tcaccggcgc
    17941 ggatggcaag acctacgaga agaccatcga ggtcaacaag cctgtcgagg tggacggcat
    18001 catgctctat caggcgagct tcgaagatgg cggctcgcgc ctgcgcctgc tggcacgcga
    18061 cctgcgcgtc gacggcacgc cggatcagtc cgttgacgtc gccgtcggtg attcgctgcc
    18121 cctgaagcgc aatggcctgg acctgaccct ggagctcatt gccttccgcc ccttcaacgt
    18181 ggaaaacctc gccgatcccg acacgggcgc ggcgtccggc accctcggca cgctggagaa
    18241 gcatctgggc agcggtgctc gcagcacggc gccgaaggat cttcacaatg taggcccgag
    18301 ctttcagtac aagctgcgcg acaacaccgg ccaggcgcgg gaatatcaga cctacatgca
    18361 gcccatcgcc caggacggtg cctggtatct gctcgccggc atgcgcgaca cgccggacgc
    18421 gcccttccgc ttcatgcgca tcccggtgga cgccgatcag aaggctgatg cctggttcgc
    18481 cttgcgccgg gtgctcctcg acccccgccg ccatgacgag ttggcccgcc gctttgccgc
    18541 cactgccctg gaccggggcg acgacgcagc cctgcgtgaa cgcctgcagg aaaccgcccg
    18601 tcgcaccctc gacctgttcg cccgcggcgg cttcgagagc gtcggcaaat tcatcgaaag
    18661 caccatcccg gaaggcgagc gcgaccgcgc cgccggcgtc ttcatcaagg tcctggaagg
    18721 ggtgacctgg gaagcctggc agctcgcccg tcaggacgcc ggcctgccgc ccctcaagaa
    18781 cgacgcgcaa tacgcccgtt tcgtgcgtga cagcctcaac gccgccagcg acagccagca
    18841 ctacggcgcg cccctcctct tccagctcga ccgcttcgac gaagtgcgcg ccacggtcct
    18901 ccaggccacc cgctcaccgg gcaagcccat cgtctatctc ggctccctgc tgctggtgct
    18961 gggcgtgttc gccatgctct acatccggga acgccgcctc ttcgtgctcg tcaaacactc
    19021 tggcgaggcc ctcgtggccc tcagctccaa ccgcaagtcc ctggacgtgg acacggcctt
    19081 cgccgcccac cgggacgccc ttgccacccg cttcacccct ccggcagacg ctccacctgc
    19141 catcgaaccg aggtaatccg catggacatc gcccacccgg acactccttc cctgctgcgc
    19201 cgcctccagc ccggcgactg gctgtatgcc ctggccgtgg cgggcggcgg catgtttgcc
    19261 ctgcgtcagt acggtggctt catggacatc tacgacaagg ccatcctggc cctcgtggtg
    19321 ccggccgtcg tcgccgcagg ctggcactgg aagcccttcc gcctcttcct ggccgccgtc
    19381 gccgccctcg ccctgacaag cgtcgggctt tacgaaggcg atctggcgcg ggccgaaaag
    19441 atcttcctgc tcaagtattt cctgtccagc cagaccgcca tcatgtggat gagcgccctg
    19501 gtcttcctcg ccaccggaag ctacggcctg ggcctcgccg cccgctccga cttcgccgag
    19561 aagaccggct ccgcgctgac ctggacctcc gccaccttcg gcaccacggg tctcttcgtg
    19621 cgctggtacg agagctacct gatcggccgc gacgtgggcc acatccccct cagcaacctg
    19681 tacgaagtct tcgtgctgtt caccctgctc acgtccctgc tctacctgta ttacgagggc
    19741 cgctacgcca cgcggcggat gggcgccttc gtgctgctgg tgatctcggc cgcggtgagc
    19801 ttcctgctct ggtacacctt ttcccgcgaa gcgcaggaga tccagccgct gatcccggcg
    19861 ctgcagagtt actggatgaa ggtccatgtg ccggccaatt tcatcggcta cggcgccttt
    19921 gcgctggccg cgatgatcgg ggtggcggac ctgctggtgc ggcgcggcct gctcgccagc
    19981 cgtctaccgg cgcccgaggt gctggacgac gtgatgtaca aggccatcgc catcggtttc
    20041 gccttcttca ccattgccac catcctcggc gccctgtggg cggcagaagc ctggggcacc
    20101 tactggcagt gggatcccaa ggaaacctgg gccttcatcg tgtggctcaa ctacgccgcg
    20161 tggctgcaca tccgcctcac caagggcctg cgcggcgcgc tgctggcctg gtgggcggtg
    20221 gtgggactgc tggtgacggc cttcgccttt gtcggcgtga acatgttcct gtccggcctg
    20281 cactcctatg gctcgctctg aggcaccttg ccgcgcggac gtccggcctc cagccggacg
    20341 atctccgcgg cactgaagcc cgcccgccgg cgggcttcaa cgttcagggg cccgggcggc
    20401 cacggcgcgt cgaaatccgc catcagcgcc agataggttg cttcgggctc aagcccgcgc
    20461 tccgcgcaca ggtggcggaa ccagcgatcc cccagggcca cgtggcccac ctcgtcccgc
    20521 aggatgatgt ccagcacggc gatgctgtcc gcgtcgccga tgcggtgcag ggccgccacg
    20581 atgggcggcg tggcgtccag gccacgggct tccaacacgc gcggcaccag ggccatacgt
    20641 gcaagggggt cctcagcggt cttcaccgcc atgtcccaca ggccccgatg ggccgggaaa
    20701 tcgccgtagg catgccccag cgcggccagc cgggtgcaca cccgcgcgaa gtgatccgct
    20761 tcctccgccg caacctgcag ccagccggcg taaaactcgt ccggcaggct acggaagcga
    20821 tacacgcagt ccagggccaa gttgatggca ttgaactcga tgtgagcgat ggcgtggagg
    20881 agcgccgcgt ggccttccgg cgtggtgggc ttgcgacggg gcaggtcggt gtgaggcagg
    20941 ag
//
LOCUS       DAOQPA010000056        20636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1259_length_20636_cov_17.598222, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000056 DAOQPA010000000
VERSION     DAOQPA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20636
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1259_length_20636_cov_17.598222"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKX01_09420"
     CDS             complement(<1..589)
                     /locus_tag="PKX01_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMZ76387.1"
                     /translation="MTVLETQGFKLDNSGKRVVVDPVCRIEGHLRVEVNIDDKNVIRN
                     AVSTGTMWRGLEVILKGRDPRDAWAFTERICGVCTGTHALTSVRAVEDALAIQIPENA
                     NIIRNLMQLNLYVHDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPLSSPG
                     YFRDVQNRLKKFVESGQLGPFMNGYWGNPAYKLPPE"
     gene            complement(589..1677)
                     /locus_tag="PKX01_09425"
     CDS             complement(589..1677)
                     /locus_tag="PKX01_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HMZ76388.1"
                     /translation="MTETFYDVLRRQGITRRSFMKFCSLTATSLGLGSAFVPKIAHAL
                     ETKPRTPVIWLHGLECTCCSESFIRSAHPLTKDVVLSMLSLDYDDTLMAAAGHQAEAI
                     IEEVKKKYKGNYIVAVEGNPPLNEDGMFCIHGGRPFVEVLKETCADAKAIISWGACAS
                     YGCVQAAKPNPTRATPVHKVITDKPIVRVPGCPPIAEVMTGVVTYMLTFDRIPELDRQ
                     GRPKMFYGQRIHDKCYRRPHFDAGQFVESWDDEAARKGYCLYKMGCKGPTTYNACSSM
                     RWNGGVSWPVQSGHGCIGCSEEGFWDKGSFYDRVTDIKQFGIESNADKVGGAAAGVVG
                     AAVAAHAAVTALARARAKQGEDQNAGGQ"
     gene            2191..3666
                     /locus_tag="PKX01_09430"
     CDS             2191..3666
                     /locus_tag="PKX01_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WSC domain-containing protein"
                     /protein_id="HMZ76389.1"
                     /translation="MHTRQSLIHLLVLTALAGSPPAWAADIGFDDVPSGTLVDGTYAG
                     SGVLLSSVASQSGHAYASAWQNSNTVTLWNPATGGAFSSAFSADEGAVKAVFAAPQKK
                     VSIDARGVEAIEYLGNDASLQRRPFLEAFDASGKLLAKVYYANWYGDGKAGWGTWQTL
                     TVERPSADIAYVLFSSIANPPGQRGRVNGQGIYGVFDNLQTYPQTQGAYVGCYTDDGV
                     RALPVQLMAAGATVESCMAAAKAHTYAYAGLQYGGACFAGNAPALRKVADSECAMPCS
                     ADKSESCGGSWRNSVYATGVTPAAPAKPVYQGCYTDDASRALPVTLMANSIANVDTCV
                     AAARARNLAYAGLQYGGQCYGGNSLGKTKVADNECNMPCTAGAGQTCGGTWRNSVYAT
                     GVPSLPKPPANAYKGCYVDTASRTLPIALMSSGATVESCVAAAGREGYAYAGLQWQGQ
                     CYAGNTLPPTKAADAECNTPCIANSTETCGGGWRNSVYATH"
     gene            complement(3773..3862)
                     /locus_tag="PKX01_09435"
     tRNA            complement(3773..3862)
                     /locus_tag="PKX01_09435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3826..3828),aa:Ser,seq:cga)
     gene            complement(4074..4439)
                     /locus_tag="PKX01_09440"
     CDS             complement(4074..4439)
                     /locus_tag="PKX01_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ76390.1"
                     /translation="MTSKSFQTITADLSRAVDSLRAEIPGTMQGFSSMARDAMKPGVL
                     SALDKELLATAIGVAARCDGCVGFHVKALIRLGVTREQLMETLAVAVYMGGGPSLMYA
                     AEAVRAYDEFIDLPIRQAA"
     gene            complement(4569..5855)
                     /gene="serS"
                     /locus_tag="PKX01_09445"
     CDS             complement(4569..5855)
                     /gene="serS"
                     /locus_tag="PKX01_09445"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMZ76391.1"
                     /translation="MLDVQLLRTRLDFVAERLATRGLQLDVAAFQALEDERKTRQTRT
                     QELQARRNALSKQVGQLKGKGEDASAVLAEVASIGDELKANEIALAELLERFNAFVAG
                     LPNLPHESVPPGGSEADNVEVSRWGAIPTFDFEVRDHVDLGARLGGLDFETAAKISGA
                     RFTLMKGGLARLHRALAQFMVDTHTAEHGYTELYVPYLVNAASMFGTGQLPKFEEDLF
                     PVPRADGGKFYLIPTAEVPVTNIVRDELLSADALPLKFVSHTPCFRSEAGSYGKDTRG
                     MIRQHQFDKVELVRIEHPDQAWAALEELTGHAEAILQKLELSYRKIVLCSGDMGFSSA
                     KTYDLEVWLPAQNTYREISSCSCFEGFQARRMQARFKNAQGKNEAVATLNGSGLAVGR
                     TLVAVLENYQQADGSVRIPTVLQPYMGGVTMLAPIN"
     gene            6117..6386
                     /locus_tag="PKX01_09450"
     CDS             6117..6386
                     /locus_tag="PKX01_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76392.1"
                     /translation="MLSIRDCLDYCDLTDEDVAIIAEHHDIPEAAAAQIACGLVQTPE
                     GTLMLTQFLLDLVDRAEERGDRANANRFRAACARFIQAHPVSHTH"
     gene            complement(6383..7711)
                     /locus_tag="PKX01_09455"
     CDS             complement(6383..7711)
                     /locus_tag="PKX01_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMZ76393.1"
                     /translation="MADLFDALESPRVPLAEAMRPRTIADVVGQAHLLGPGKPLALAF
                     QSGKPHSMILWGPPGVGKTTLARLMAQAFDAEFVALSAVFSGVKDIREAIARAEVERA
                     RGRHTILFVDEVHRFNKAQQDAFLPFVEQGLVTLVGATTENPSFEVNSALLSRAAVYV
                     LQPLTPEELVVLFERARAHACPQLAFEPDARDRLVGFADGDARRLMNLLEQVHTAADT
                     AGQNPVTPAFVDEALSRNLRRFDKGGEAFYDQISALHKSVRGSDPDASLYWLCRMLDG
                     GADPHYLGRRLVRMAVEDIGLADPRALEISLNACATYERLGTPEGELALAEAAVFLAC
                     AAKSNAVYKAYNAARKFVAADASRPVPMHLRNAPTRLMKDLGYGKAYRYAHDEPDAYA
                     AGESYLPDGMARPDWYVPTGRGLEAQIADKLARLRERDRAAQDADRRDEV"
     gene            complement(7720..8370)
                     /gene="lolA"
                     /locus_tag="PKX01_09460"
     CDS             complement(7720..8370)
                     /gene="lolA"
                     /locus_tag="PKX01_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HMZ76394.1"
                     /translation="MMIRKMSCAALGLVALLSAGAARADGLAQLKQFMEGTRAAKGTF
                     TQQVFAKSGHKPQQAGGTFAFSRPGKFRWVYDKPYAQVLVGDGRTLWAFDPELNQVTT
                     KKLGQALGSSPAAILAGDNALERSFVLKDAGVADGLEWVEATPKGEDSSFERIRLGLS
                     GGQLRAMLLFDNFGQTTSVQFSQIERNPALDAALFHFVPPKGADVVGDGSPAGAAR"
     gene            complement(8367..10658)
                     /locus_tag="PKX01_09465"
     CDS             complement(8367..10658)
                     /locus_tag="PKX01_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HMZ76395.1"
                     /translation="MLNRPSTRPVPLPEKIASLLQEARWLLLGVLAAYLAIVLFGYAK
                     SDPGWSHAVYVERVSNPGGRFGAWLSDLLLYLFGLSAWWWVAFLGVGLAVGFRRLQRE
                     HGADRRSLFIVLAGFLVVLVASSGLESMRFHSLRATLPLEPGGLIGVELGSVVGIYLG
                     FTGGTLLLLTLIAAGLSLFSGVSWVEASEAVGGALEAAWNFVLHGAQTWEDRRVGREV
                     AQKREAVVETKRKKEEAAPPPPVRIEPAVVEVPRSERVVKERQAALFVELPGGSLPPL
                     ALLDEPADDVEPPSVESLEFTSRLIERKLADFGVEVKVLAAYPGPVITRFEIEPAVGV
                     KGSQVMNLVKDLARALSVVSIRVVETVPGKTCMALELPNPKRQTVRLSEILGSKAYHD
                     MHSPLTVALGKDIGGQPVVADIAKTPHLLVAGTTGSGKSVGINAMILSLLYKSEPEDV
                     RLILVDPKMLELSIYEGIPHLLAPVVTDMKHAGNALNWCVAEMEKRYKLMSSVGVRNL
                     AGYNKAVAEAVKAGAPLKNPFSITPESPEPLEKLPYVVVIIDELADLMMVVGKKIEEL
                     IARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRMAFQVSSKIDSRTILDQMGA
                     EALLGMGDMLYLAPGTGLPTRVHGAFVADHEVHKVVEFLKSTGAPDYVEGILAPQDEE
                     AGDLLGGGEGGEGSEADPLYDQAVEVVLKTRRPSISLVQRHLRIGYNRAARLIEQMER
                     SGVVSAMSANGNRDVIVPPREGE"
     gene            complement(10798..11469)
                     /locus_tag="PKX01_09470"
     CDS             complement(10798..11469)
                     /locus_tag="PKX01_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ76396.1"
                     /translation="MSVNSTVSTIALRTFPIFQGLGDDRLAGVARCAMMRRVPRGSAV
                     VHEGDRTDFVYFVLTGSLKVMVSDEDGREVILKILGQGEMFGEMGVLDDSPRSASVVA
                     VSPSDLVTISKTDFKRLMQENFELAWHVMCNLAHRLRDADRKIESLALMDVYGRVARL
                     LLEMAEEVDGVKVVRKKISKQDIAKMIGASREMVSRVMKDLGLRGLIEETDGGVILRE
                     KIYDL"
     gene            11687..12646
                     /gene="trxB"
                     /locus_tag="PKX01_09475"
     CDS             11687..12646
                     /gene="trxB"
                     /locus_tag="PKX01_09475"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMZ76397.1"
                     /translation="MATRHIKLLILGSGPAGYSAAVYAARANLKPVLITGIAQGGQLM
                     TTTEVDNWPADADGVQGPELMARFEKHALRFNTEMVFDHIHTTYLAEKPMRLVGDAGE
                     YTCDALIIATGATAKYLGLPSEEAFSGKGVSACATCDGFFYKNQEVAVIGGGNTAVEE
                     ALYLANIASKVTLVHRRDKFKAERILIDKVYEKVAAGKIVLELDSTLDEVLGDASGVT
                     GMRVRNVNSGAARDIALKGVFIAIGHKPNTDIFEGQLEMEGGYIVTHGGRNGNATATS
                     VPGVFAAGDVQDHVYRQAITSAGTGCMAALDAERYLDGLGLAG"
     gene            12720..13703
                     /locus_tag="PKX01_09480"
     CDS             12720..13703
                     /locus_tag="PKX01_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ76398.1"
                     /translation="MAKDKPRIGLDALKALKTAGGARSASVPGRPAAPAPPAQASDTD
                     AALFRREVGRVRPLKAEDYADLSRPRPAPLPRPKTPESTVPDTPRRPSRVDENDPAAV
                     FRAALGDIAPIRDFNRADLSDQRRDTRLGSPPHTDAAPAPVDLNTLHDPAALFRAVAG
                     DVAPLKGSNRADLDRPLPAPHPRQREEDEREVLREALETPLTFEDRLDMGDEAAYLRS
                     GLPRRVLTDLRRGRWVVQGELDLHGLTRDEARAALARFIADALLRGRRCLRVIHGKGH
                     GSPGRIPVLKHLSRGWLAQREDILAFCQARPHDGGDGALLVLLRAGTPVRG"
     gene            13988..17194
                     /locus_tag="PKX01_09485"
     CDS             13988..17194
                     /locus_tag="PKX01_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMZ76399.1"
                     /translation="MDTRRHRRHRSRLALALAGAFAGFAGLTPSLQAAQFAVGTFAPW
                     PGYAIPNGQWMSADVNGDGKADIIHAVAGADYVNTWLSNGNGSYTVKSFRPWSGYGIP
                     NGVWLTGDINGDGKADIVHAVANTDYVHTWMSNGDGTYTMGTFRPWAGYGIPNGEWRI
                     ADVNGDGKADLIHAVAGADYVNTWLSNGNGTFTVKSFRPWAGYGIPNGVWLTADLNGD
                     GKADIVHAVANTDYVHTWMSRGDGTYTMGTFRPWAGYGIPNGQWMTGDVNGDGKTDLI
                     HAVAGADYVNTWLSNGSGGFTVRSFRPWAGYGIPNGEWRVADLDGDGKADIFHAVANT
                     SYAHQWLSNGDGTYRVQTFSPWAGYAIPNGVWLTGDFNGDRKTDIIHAVANTNYVHTW
                     RSTLPRPGELSLDGLEITQSVQNMAQEVGLVADKRTIARAYVSINGGAAVTVRGVLTA
                     TRTGAAPVSITSLNTVTLDPALAGNLRAKRENLASSLNFEVPPALRSAGATQFRITVQ
                     RVSPGAALVCADCDSSPVGVSFATAAPLRVRVLGLRYSSGTPATVKSPRALDYTLVRS
                     WLGRAYPVSTLTMSTASVNASAAWPFSCNDANAQLATLRANDVAAGTDKRTHYFGLVA
                     DGGGFMRGCAASIPGSADPTAVASGPTGTPSGSFGWDTDGSYGDWYTGHELGHTYGRL
                     HPGSGCGDSADDSHEPFTNGAISGPDGAWTGLDVGDTANGIPMAALPGAQWFDVMTYC
                     RNQWISFYTYNGIRARLTGENALPAGAPAPKALKTSAALPPMAQVGERPEPITGAKAA
                     PVLAAPRRAAPMLAAAPVTGHARPPAIIDGTASPATSTAPASEPPAAAYATPPTPVIG
                     YREVAAAPVANARAVGDDIPMQRGDLLSVVATVNLTRQTGRLVSMSRIPTGLATPVGP
                     EDARIEFLDARGKVLAAYAVPFRRDSDVEAGADQTGLIDAVVPFSTTAAKVRVSAFGT
                     LLGERDIPAKPPRLGKISVLKSKALDEGATLSWTAVAGPLVSYSVQSSEDGGKSWQTL
                     AIGLREPRFQLPPAEGGVTPRYRVIAHDGIHNTIVPVR"
     gene            17478..19424
                     /gene="nosZ"
                     /locus_tag="PKX01_09490"
     CDS             17478..19424
                     /gene="nosZ"
                     /locus_tag="PKX01_09490"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HMZ76400.1"
                     /translation="MSTEHHPQDDQDLVPGRRKFLNTAALAGLAGAGLSVGLSACKQE
                     AAPTTAPAAPAPAAKAAEGGELPIHPAPGQLDSYYGVWSGGHSGEVRILGLPSGREIK
                     RVPVFNIDCMSGWGITNESKAVIGTKADGSLKYKTGDTHHVHGSYTDGTYDGKYFWVN
                     DKIHGRLARIKGDTFECDKITEIPNIQGFHGIFPDKRDPVDQAINHTTRVFCGNEFHV
                     PQPNDGRDEDDATKYHCLFTCIDAETMEVRWQCRVDGNMDLVASSFDGKLAASNQYNT
                     EGGAHYEDMMSAERDACVFFHIERIEAAIKAGKASTIGDSKVPVVDGTRAANPDPKTA
                     LTCYVPVPKNPHGVNASPDGKYFICSGKLSPTATVIELSQVLAYFDGTLDDLKKAIVA
                     EVELGLGPLHTAFDGRGNGFTTLFLDSQVVKWNIDQAIKFYAGDKNAKYVVDRVDVHY
                     QPGHINASMSETKEADGQFLGVGCKFSKDRFLPVGPLHPENEQLIDIRGEKMKLLADH
                     PVYPEPHDFIIVKRDKIKTRQVYNVDDFPNATKDPKDSGVTRNGNKVTVRLVSQAPAY
                     SLREFKVKKGDEVTLILTNLDKVEDLTHGFAIPKYDINFIVNPQETKSVTFKADKPGV
                     YWCYCTHFCHALHLEMRTRMFVEK"
     gene            20102..>20636
                     /locus_tag="PKX01_09495"
     CDS             20102..>20636
                     /locus_tag="PKX01_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ76401.1"
                     /translation="MFSLRTLAAALATAAACLLAGAAQADTYEVKLPPELFTSPRLCD
                     YAPCTDVLPGATRFSERKGQPSYVEGYKDVDGTPRLAGYVMLSTDITDIPAYSGKPVI
                     TLMGMDTTGHFTGVRILKHSEPILLLGIPEAALVRFNNQYLGKFVGDNIEIGKSRPED
                     NLIGLDAITGATVTVIAQ"
BASE COUNT         3670 a         7465 c         6312 g         3189 t
ORIGIN      
        1 cttcgggcgg cagcttgtag gccgggttgc cccagtagcc gttcatgaag gggcccagtt
       61 ggccggattc gacgaacttc ttcaggcggt tctgcacgtc gcggaagtag cccggcgacg
      121 acaggggcca gctcgagatg ctctgggcca gctcggaggt cttcttggga tcggccttca
      181 gcgccgacac cacgtccacc cagtccagcg cgtgcagatg gtagaaatgc acgaggtggt
      241 catggacgta gaggttcagc tgcatcaggt tgcggatgat gttggcgttc tccgggatct
      301 ggatcgccag cgcatcctcc accgcgcgca ccgaggtgag ggcgtgcgta ccggtacaca
      361 cgccgcagat ccgctcggtg aaggcccacg cgtcgcgcgg gtcgcggccc ttgaggatga
      421 cctcgagccc gcgccacatg gtgccggtgg acaccgcgtt gcggatcacg ttcttgtcgt
      481 cgatgttcac ttccacacgc aggtggccct cgatccggca caccggatcc accaccacgc
      541 gcttgcccga gttgtcgagc ttgaagccct gggtttcaag cacagtcatc attgacctcc
      601 cgcattctga tcttcaccct gcttcgcccg cgcccgggcc agcgccgtca ccgccgcatg
      661 ggcggccacg gccgcgccca cgacgccggc cgcagcgccg cccaccttgt cggcattgct
      721 ctcgatgccg aactgcttga tgtcggtgac gcggtcgtag aagctgccct tgtcccagaa
      781 gccttcctcg gaacagccga tgcagccgtg accggactgg accggccagg agacgccgcc
      841 attccagcgc atggacgagc aggcgttgta ggtggtcggg cccttgcagc ccatcttgta
      901 gaggcagtaa cccttgcgcg ccgcctcgtc gtcccagctc tcgacgaact ggccggcgtc
      961 gaagtggggc cggcggtagc acttgtcgtg gatgcgctgg ccgtagaaca tcttcgggcg
     1021 gccctgacgg tccagctccg ggatgcggtc aaaggtcagc atgtaggtca ccacgccggt
     1081 catcacttcg gcgatgggcg ggcagcccgg cacgcgcacg atgggcttgt cggtgatcac
     1141 cttgtgcacc ggcgtcgccc gggtcgggtt gggcttggcc gcctgcacgc agccgtagga
     1201 tgcgcaggcg ccccagctga tgatggcctt ggcgtcggcg caggtctcct tgagcacctc
     1261 cacgaagggc cggccgccgt ggatgcagaa catgccgtcc tcgttgaggg gcggattgcc
     1321 ctccacggcg acgatgtagt tgcccttgta cttcttcttg acctcttcga tgatcgcctc
     1381 ggcctggtgg ccggcggccg ccatcagcgt gtcgtcgtag tccagcgaca gcatggacag
     1441 caccacgtcc ttggtgaggg gatgggccga gcggatgaag gactcggagc agcaggtaca
     1501 ttccaggccg tgcagccaga tcaccggcgt gcgcggcttg gtttccagcg cgtgggcaat
     1561 cttcggcaca aaggcgctgc ccagcccgag ggacgtggcc gtcaggctgc agaatttcat
     1621 gaagctgcgc cgggtgatcc cctgccggcg cagcacgtcg taaaaggttt cagtcatgga
     1681 acccggctcc cctcgcggat gaatcgcgtc tgaacgtcat gtgggtgcgc ggccgaccgc
     1741 ttgtggcggg gtcgtttgcc gcatcgcgag gagacaccga cacaagatcc gtgcccgcac
     1801 cacgcgaaac ataaaaccgc ttttcgatca gggtcttgtc tgcatgcacc ggcgtccgcc
     1861 gggccgtcct tgcaagctgc actgccagca aagcggaagg ctactttccg gtgagcgtag
     1921 ccgcccgcag gcacgcacgg cgcccgggaa tgacgcgcgc atccgccctg aagccccggc
     1981 gcgaccgctc gacccggccc tgtaacctgg ctgcgcctga aacccggaat gggtacatga
     2041 acagcgctcg ccacaccgag ggttgaccgg ggtcaacgac acccctgcag gccgggccga
     2101 ccgtgaacct gtcagtcacc ccttgggcct tgccgcgtcg catggcccgg ccaggcccgc
     2161 tttccccaac gaatcgaagc ggaggatgtc atgcacactc gccaatccct gatccacctg
     2221 ctggtcctca cggcgcttgc aggcagccca cccgcgtggg cggcggacat cggcttcgac
     2281 gatgtcccgt cgggcaccct ggtggatgga acctacgccg gcagcggcgt cctcctttcc
     2341 agcgttgcca gccagagcgg tcacgcctac gcctctgcgt ggcagaactc gaacaccgtc
     2401 accctgtgga atcccgccac gggcggcgct ttctcgtcgg ccttttcggc cgacgagggg
     2461 gccgtcaagg ccgtattcgc agccccgcag aagaaggtca gcatcgatgc acggggcgtg
     2521 gaagccatcg aatacctggg caacgacgcc agcctgcagc ggcgcccctt cctggaagcc
     2581 ttcgacgcga gcggcaaact cctggccaag gtgtattacg ccaactggta cggcgacggc
     2641 aaagccggct ggggcacgtg gcagacactg accgtcgaac gcccgagcgc cgacatcgcc
     2701 tacgtcctct tctcgtccat cgccaacccg cccggtcagc gcggtcgcgt gaacggccag
     2761 ggtatctacg gcgtgttcga caatctgcag acatatccgc aaacccaggg tgcctacgtg
     2821 ggctgctaca cggatgacgg cgtgcgcgcg ctgccggtgc agctcatggc cgcgggcgcc
     2881 acggtggaaa gctgcatggc ggcagccaag gcccacacct acgcctacgc gggcctgcag
     2941 tacggcggcg cctgctttgc cgggaacgct ccggccctcc gcaaggtcgc cgacagcgaa
     3001 tgcgcgatgc cctgctcggc cgacaagtcc gagtcctgcg gcggaagctg gcgcaacagc
     3061 gtgtacgcca ccggcgtcac acccgccgcg cccgccaagc cggtctatca gggctgctac
     3121 accgacgatg ccagtcgggc attgcccgtc accttgatgg ccaacagcat cgccaacgtc
     3181 gacacctgcg ttgccgccgc ccgtgcccgc aatctggcct acgccggcct gcaatatggt
     3241 ggtcagtgct acggcggcaa cagcctcggc aagaccaagg tggcggacaa cgagtgcaac
     3301 atgccctgca ccgccggcgc cggtcagacc tgcggcggca cgtggcgcaa cagcgtctac
     3361 gcgacaggtg ttccgagctt gccgaaaccg cccgccaacg cctacaaggg ctgctacgtg
     3421 gataccgcca gccggaccct gccgatcgcc ctgatgtcct ccggcgctac cgtggaatcc
     3481 tgcgtcgccg ccgccggacg ggaaggctac gcctacgccg gcctgcaatg gcaaggtcag
     3541 tgctacgcgg gcaacacgtt gccgcccacc aaggctgcag acgccgaatg caatacgcca
     3601 tgcatcgcga acagcaccga aacctgcggc ggcggctggc gcaacagcgt gtacgccacc
     3661 cactgaaagc atgcagggca cccggtcgca ggcgcggacg cgccctgcga ccgacaccac
     3721 aggatcgcag aaacgaaaaa acgcgccgaa aggcgcgttt ttacgggatg cttggcggaa
     3781 agggtgggat tcgaacccac ggtacagcaa gctgtacacc ggatttcgag tccggcccat
     3841 tcgaccactc tggcaccttt ccgctcgaag ccgttgattg taaccgaatc gtccgggaat
     3901 gtcagtcccg ataccgcggg gaatgtcgaa agggggccgt ccggcggatg tgctgcggca
     3961 gcgcggccac gcccctcgac gccttacgcg cgcttcaaaa cacgggcggg cgccatcgag
     4021 ccggacatca cgccagcgtg atacgaggag cggagcatcc ccggagcgcc agctcaggct
     4081 gcctggcgaa tcggcagatc gatgaattcg tcgtaggcgc gcacggcctc ggcggcgtac
     4141 atcaggctcg ggccgccccc catatacacg gcgaccgcaa gggtctccat cagctgttcg
     4201 cgtgtcacgc cgaggcggat cagcgccttg acatggaagc cgacacagcc gtcacaacgg
     4261 gccgcaacgc caatggcggt ggccaggagt tccttgtcga gcgcggacag gacaccgggc
     4321 ttcatggcgt cgcgggccat gctgctgaag ccctgcatcg taccggggat ctcggcacgc
     4381 aggctgtcca ccgcccggga cagatcggcc gtaatggtct gaaaggattt gctcgtcatg
     4441 ggggtgcctc atcgttttgt tgtgaaagaa tgtcaaaacg atatcagccc accccctctt
     4501 cgggtcaacg caagactccc acctgtcatt taaatccgag gacaaaggaa ttcgatcacg
     4561 ctgccggatc agttgatcgg cgccagcatg gtcacgcccc ccatgtaggg ctgcagcacc
     4621 gtcgggatgc gcaccgagcc gtccgcctgc tggtagtttt ccagcaccgc cacgagggtg
     4681 cgccccaccg ccaggccgga gccgttgaga gtcgccacgg cttcgttctt gccttgggcg
     4741 ttcttgaagc gggcctgcat gcggcgtgcc tggaagccct cgaagcagga gcaggaggag
     4801 atttcccggt aggtgttctg ggccggcagc cagacctcca ggtcgtaggt cttggccgac
     4861 gaaaagccca tgtcgccgga acacagcacg atcttgcgat aggagagttc cagcttctgc
     4921 aggatggcct cggcgtggcc ggtgagttcc tccagcgccg cccaggcctg gtcgggatgc
     4981 tcgatgcgga ccagctccac cttgtcgaac tggtgctggc ggatcatgcc gcgcgtgtcc
     5041 ttgccgtagc tgccggcttc ggagcggaag cacggggtgt ggctgacgaa cttgaggggc
     5101 agcgcatcgg ccgacaggag ttcgtcgcgc acgatgttgg taaccggcac ctcggcggtg
     5161 gggatcaggt agaacttgcc cccgtcggca cggggcaccg ggaacaggtc ttcctcgaat
     5221 ttgggcagct ggccggtgcc gaacatgctg gccgcattca cgaggtaggg cacgtagagc
     5281 tcggtgtagc catgctcggc ggtgtgggtg tccaccatga actgggccag agcccgatgc
     5341 aggcgggcca gaccgccctt catcagggtg aagcgcgcac cggagatctt ggccgccgtc
     5401 tcgaaatcca gcccgccgag gcgggcgccc aggtccacgt ggtcgcgcac ctcaaagtcg
     5461 aaggtcggga tcgcacccca ccggctgacc tccacgttgt ccgcctccga accgcccggc
     5521 ggcacgctct cgtggggcag gttgggcagc ccggcgacga aggcgttgaa gcgctccagc
     5581 agctcggcca gggcaatctc gttggccttg agttcgtcac cgatgctcgc cacctcggcc
     5641 agcaccgcgg aggcgtcctc gcccttgccc ttcagctggc cgacctgctt ggacagggca
     5701 ttgcgccgtg cctggagttc ctgggtgcgg gtctggcgcg ttttgcgttc gtcttcgagc
     5761 gcctggaagg ccgccacatc gagttgcagg ccgcgggtcg caaggcgttc ggcgacaaaa
     5821 tccaggcggg tgcgcagcag ttgtacgtca agcatgggga atcctattca aatcgaaaat
     5881 ccggccgaca tcatattcgg cataaatatt gcttggcaga ccgcgaacgt ttccgctata
     5941 ttgcgacgca tcacaaagcg cgtgacgcat tccacgggct cgttcaatgg aaattggaaa
     6001 gttcatagcc tgcccgctgt gaaacttttc ttgaatttgt gtattcagag gtcacaattg
     6061 aagaaggctc ccggcagaag gaacccttcc tcgtccgacc atcgaaggag actccaatgc
     6121 tatccatacg cgactgtctg gattactgcg acttgaccga tgaagacgtc gcgatcatcg
     6181 ccgagcacca cgacattccc gaagccgccg ccgcccagat cgcctgcggg ctggtccaga
     6241 ccccggaagg cacgctgatg ctcacccagt tcttgctgga tctggtggat cgggccgagg
     6301 aacggggcga ccgggccaac gccaatcgct tccgagccgc ttgcgcacgc ttcatccagg
     6361 cgcatccggt gtcgcacacc cattagactt cgtcccgcct gtccgcatcc tgcgccgcgc
     6421 gatccctttc gcgcaggcgc gccagcttgt ccgcaatctg cgcttccagc ccgcgcccgg
     6481 tcgggacata ccagtctggt ctcgccatgc cgtcgggcag ataactttcc cctgccgcgt
     6541 aggcgtccgg ctcgtcgtgg gcatagcggt acgccttgcc gtagccgaga tccttcatga
     6601 ggcgtgtcgg cgcgttgcgc aggtgcatcg gcaccggacg cgatgcgtcg gccgccacga
     6661 acttgcgggc tgcattatac gccttgtaca ccgcgttcga tttggccgcg caggccagaa
     6721 agaccgccgc ttcggccagc gccagctccc cttccggggt gcccaggcgc tcgtaggtgg
     6781 cacacgcgtt caggctgatc tccagcgccc gcggatccgc gagaccgata tcttccaccg
     6841 ccatgcgcac cagccgccgt cccagatagt ggggatccgc cccaccatcg agcatgcggc
     6901 acagccagta gagggatgca tccggatcgg agccccgcac ggatttgtgg agcgccgaaa
     6961 tctggtcata gaaggcctcg ccgcccttgt cgaaccgccg caggttgcgc gacagggctt
     7021 cgtccacgaa ggctggcgtg accgggttct gtccggccgt atccgcagcg gtgtgtacct
     7081 gctccagcag attcatcaga cgccgcgcat cgccatcggc aaagccgacg agacggtcgc
     7141 gggcgtcggg ctcgaaggcg agctgcgggc aggcgtgggc ccgcgcccgc tcgaagagga
     7201 cgacgagttc ctcgggcgtg agcggctgca gcacatacac cgcggcccgt gacagcagcg
     7261 ccgaattcac ctcgaaggac ggattctccg tcgtcgcccc caccagcgtg accagcccct
     7321 gctccacgaa gggcagaaag gcgtcctgct gggccttgtt gaagcgatgg acctcgtcca
     7381 caaagagaat ggtgtgccgg ccgcgggcgc gctccacctc ggcccgggcg atggcctcac
     7441 ggatgtcctt gacccccgag aacaccgccg acagggcaac aaactccgcg tcgaaggcct
     7501 gggccatgag ccgcgcgagg gtggtcttgc caacacccgg cgggccccac aggatcatcg
     7561 aatgaggctt gccggactgg aaggccaggg ccagcggctt gccggggccg agcaggtgag
     7621 cctgaccgac cacgtcggca atcgtccgcg gacgcatcgc ctccgccagc ggcacccgcg
     7681 gcgactcgag ggcatcgaag agatcggcca tcctgctgcc tagcgggctg cgccagcggg
     7741 gctcccgtcg cccaccacgt cggccccctt ggggggaacg aagtggaaca gggcggcatc
     7801 gagcgccgga ttgcgctcga tctggctgaa ctgcaccgag gtggtctggc caaagttgtc
     7861 gaacaacagc atggcccgca actgcccacc agatagcccc aggcggatgc gctcgaaact
     7921 gctgtcctcg cccttgggcg tggcctcaac ccactccagc ccgtcggcaa cgccggcgtc
     7981 cttgagcacg aagctgcgct ccagcgcatt gtcgcccgcc aggatggccg ccgggctgct
     8041 gcccagggcc tgacccagct tcttggtggt gacctggttc agctccggat cgaaggccca
     8101 cagggtacgg ccgtcgccga ccagcacctg ggcatagggt ttgtcataga cccaacggaa
     8161 cttgcccggc cgggaaaagg caaaggtgcc cccggcctgc tggggcttgt ggccggattt
     8221 ggcgaacacc tgctgggtga aggtgccctt cgccgcgcgg gtgccttcca tgaactgctt
     8281 cagctgggcc agcccatcgg cacgggccgc cccggcagac aacagcgcga ccagcccgag
     8341 ggcggcgcag gacatcttgc ggatcatcat tctccttcgc ggggcggcac gatcacgtcc
     8401 cgattgccgt tggcgctcat ggccgacacc acgcccgaac gctccatctg ctcgatgagt
     8461 cgggcggcgc ggttgtagcc gatgcgcagg tgacgctgca ccagggagat ggacggccgg
     8521 cgcgtcttga gcaccacctc cacagcctgg tcgtagaggg gatcggcctc gctaccctcg
     8581 cccccttcgc caccgccgag cagatcgccc gcctcctcgt cctgaggcgc cagaatgccc
     8641 tccacgtagt ccggtgcacc ggtgctcttg aggaattcca ccaccttgtg cacctcgtgg
     8701 tcggccacga aggcgccgtg cacgcgcgtc ggcaggccgg tgccgggtgc caggtagagc
     8761 atgtcaccca tgcccagcag cgcctcggcg cccatctggt cgaggatggt gcgggagtcg
     8821 atcttgctgg acacctggaa ggccattcgc gtggggatgt tggccttgat gaggccggtg
     8881 atcacgtcca cgctggggcg ctgggtggca aggatgaggt ggatgccggc cgcccgcgcc
     8941 ttctgggcga gacgggcgat gagttcctcg atcttcttgc ccaccaccat catcaggtcg
     9001 gcgagttcgt cgatgatgac caccacgtag ggcagctttt ccagcggctc ggggctttcc
     9061 ggcgtgatgg agaaggggtt cttcagcggc gcacccgcct tgaccgcctc ggccaccgcc
     9121 ttgttgtagc ccgccagatt gcgcacgccg accgaggaca tgagcttgta gcgcttctcc
     9181 atctcggcca cgcaccagtt gagggcgttg ccggcgtgct tcatgtcggt caccaccggc
     9241 gccagcaggt gcggaatgcc ttcgtaaatc gacagctcca gcatcttcgg atcgaccagg
     9301 atcaggcgca cgtcctccgg ctcggacttg taaagcaacg acaggatcat cgcgttgatg
     9361 cccaccgact tgcccgagcc ggtcgtcccc gccaccagca ggtggggtgt cttggcgatg
     9421 tcggccacca cgggctggcc gccgatgtcc ttgccaagcg ccaccgtgag gggcgaatgc
     9481 atgtcgtggt aggccttgga accgaggatc tccgacaggc gcacggtctg gcgcttgggg
     9541 ttgggcagct ccagggccat gcaggtcttg ccgggcaccg tctcgacgac gcggatgctc
     9601 accacggaca gggcgcgggc tagatccttg accaggttca tcacctggct gcccttcacg
     9661 ccgaccgccg gctcgatctc gaaacgggtg atcaccggcc ccggataggc ggccagcacc
     9721 ttcacttcga caccgaaatc ggcgagcttc cgttcgatga ggcgggaggt gaattccagc
     9781 gactcgacgg agggcggctc cacatcgtcg gccggctcgt ccagcagcgc caggggtggc
     9841 aggctgccgc cgggcagctc gacgaacagg gcggcctggc gttccttgac cacccgctcc
     9901 gaacggggca cctcgaccac cgccggttcg atgcgcacgg gcgggggcgg tgccgcttcc
     9961 tccttcttgc gcttggtctc cacgacggcc tcgcgcttct gcgccacttc gcggccgacg
    10021 cggcgatcct cccaggtctg ggcgccgtgc agcacgaagt tccaggcagc ctccagcgcc
    10081 ccgcccaccg cctcggaggc ctcgacccag gacacgcccg agaacaggct caggccggca
    10141 gcgatgaggg tcaacaagag cagcgtgccg cccgtgaatc cgagatagat gccgaccaca
    10201 ctgcccaatt ccacgccgat cagcccaccc ggctccaggg gtaaggtggc ccgcagggaa
    10261 tggaaacgca tggactccag accgctactg gccactagca ccaccaggaa gcccgccagc
    10321 acgatgaaga gcgaacgccg gtccgcccca tgctcccgtt gcagacgacg gaatccgacc
    10381 gccagaccga ccccgaggaa ggccacccac caccaggccg acagcccgaa gagatagagc
    10441 agcagatccg acagccaggc gccgaagcgt ccgcccggat tgctcacacg ctccacgtac
    10501 accgcatggg accagccggg atcgctcttg gcgtatccga agagcacgat cgccagatac
    10561 gccgcgagca cgcccagcag cagccagcgt gcttcctgca ggaggctggc gatcttttcg
    10621 gggagcggga cgggacgggt cgaggggcgg ttcagcatgg gacggcaagg gtcgcgggaa
    10681 gcgtcgaaaa aacaaaaagc gcggtacccg accgcgcctt gattataaac cagggtctct
    10741 ggttgccccc tcctccgcaa gccgagcgat cagcgcggtg agggatttgc ggggcattca
    10801 caaatcgtag atcttctccc gcaggatgac accaccgtcc gtctcctcga tcaggccgcg
    10861 cagacccaga tccttcatca cccggctgac catttcgcgg gaggcaccga tcatcttggc
    10921 gatgtcctgc ttggagattt tcttgcgcac gaccttgacg ccatccacct cttccgccat
    10981 ctccaggagc aggcgcgcca cgcggccgta cacgtccatc agcgccaggc tctcgatctt
    11041 ccgatcggcg tcgcgcaggc gatgggccag gttgcacatc acatgccagg cgagctcgaa
    11101 gttttcctgc atgagacgct tgaagtcggt cttggaaatc gtcaccagat ccgacggcga
    11161 caccgccacc accgacgcag agcgcggact gtcatccagc acgcccatct cgccgaacat
    11221 ctcgccctgg ccgaggatct tgaggatcac ctcgcggccg tcctcgtcgc tcaccatgac
    11281 cttgaggctg ccggtcagga cgaagtagac gaagtcggtg cgatccccct cgtgcaccac
    11341 ggcggatccc cgcggcacgc ggcgcatcat ggcacagcgg gccacgccgg ccagacggtc
    11401 gtcgccaagc ccctgaaaga tcggaaacgt ccgcagggca atggtggaca cggtgctgtt
    11461 aacggacatg gagcctctcc caatgcatga aggaatggtg tgctgacccg gatggagtag
    11521 tcatacacct taacggcaga gtataaatca aacttgaatc cgtcgatggc catgcccgat
    11581 gcgtcaggat ctcgacgtgg tgcgggcctc cgctcgagcg ggcgcctccc gacccggcat
    11641 cccgacttat aatcccggct ttgctcagac cagagagaga cacgccatgg ccacccgaca
    11701 catcaagctc ctcatcctcg gttccggtcc ggccggttac agcgcggccg tgtatgccgc
    11761 ccgtgccaac ctcaagcccg tgctgatcac cggcatcgcc cagggcggcc agctcatgac
    11821 caccaccgaa gtggacaact ggccggccga cgcggacggc gtgcagggcc ccgagctcat
    11881 ggcccgcttc gagaagcacg ccctgcgctt caacaccgag atggtgttcg accacattca
    11941 caccacctac ctggcggaaa agcccatgcg tctggtgggc gacgctggcg aatacacctg
    12001 cgacgccctc atcatcgcca ccggcgccac cgccaagtac ctgggcctgc cctccgagga
    12061 ggccttctcc ggcaagggcg tgtcggcctg tgccacctgc gacggcttct tctacaagaa
    12121 ccaggaagtc gccgtgatcg gcggcggcaa caccgcagtc gaagaggccc tctatctggc
    12181 caacatcgcc agcaaggtca ccctcgtaca tcgccgcgac aagttcaagg ccgagcgcat
    12241 cctcatcgac aaggtttacg aaaaggtcgc cgccggcaag atcgtcctcg aactggattc
    12301 caccctggac gaagtcctcg gcgacgccag cggcgtcacc ggcatgcggg tgcgcaacgt
    12361 caacagcggc gccgccaggg acatcgccct gaagggcgtg ttcatcgcca tcggccacaa
    12421 gcccaatacc gacatcttcg aaggccagct cgaaatggaa ggcggctaca tcgtcaccca
    12481 cggcggacgc aacggtaatg ccaccgccac cagcgtgccc ggcgtgttcg cggcgggcga
    12541 cgtgcaggac cacgtctatc gccaggccat caccagcgcc ggcaccggct gcatggccgc
    12601 cctggacgcc gagcgttacc tggacggtct cggtctggcg ggctgaacgc gcaccggcgc
    12661 ccttcacaac ggaccggcct gcccggtccg ttgtgctttc gccctcgcga tcgacatcca
    12721 tggccaagga caaaccccgc atcggactgg acgccctgaa ggcgctcaag accgccggtg
    12781 gcgcccgcag cgcgtccgtc cctggccgtc cggctgcgcc agccccgccg gcgcaggcaa
    12841 gtgacaccga cgccgccctg ttccgccgcg aagtcggccg cgtccggccg ctcaaggccg
    12901 aggactacgc cgacctgtcg cgcccacggc ccgcgcccct gccccgcccg aagacgccgg
    12961 aatccaccgt gccggacact ccgcgccgcc cgtcgcgcgt ggatgagaac gacccggccg
    13021 ccgtcttccg ggccgccctg ggcgacatcg ccccgatccg cgacttcaac cgcgccgacc
    13081 tgagcgacca gcgccgcgac acgcgcctgg gcagcccgcc gcacaccgac gcagcgcccg
    13141 cgcccgtcga cctgaacacg cttcacgacc cggccgccct gttccgcgcc gtcgccggcg
    13201 acgtggcgcc tctcaagggc agcaaccggg cagacctcga ccggccgctg ccggcaccgc
    13261 atccccggca gcgggaagag gacgaacgcg aagtcctgcg cgaagccctc gaaaccccgc
    13321 tcaccttcga ggaccgcctc gacatgggcg acgaggctgc ctacctgcgc agcggcctgc
    13381 cccgccgcgt cctcaccgac ctgcgccgcg gacgctgggt ggtgcagggc gagctcgacc
    13441 tccacggcct cacccgcgac gaggcccgcg ccgccctcgc ccgcttcatc gccgacgccc
    13501 tgctgcgcgg ccggcgctgc ctgcgcgtca tccacggcaa gggccacggc tcgcccggcc
    13561 gcatcccggt gctcaagcac ctctcccgcg gctggctcgc ccagcgcgag gacatcctcg
    13621 ccttctgcca ggcccgcccc cacgacggcg gcgacggcgc cctgctggtg ctgctgcgcg
    13681 ccggcactcc ggtccgcggc tgatccggcg tcggcggaac gcccgcgtcg catcagcccg
    13741 atcggacacg tgtccccgtc gaagcgcgcc cgcgggccgg cgcccgaccg cctccgatca
    13801 tgcgcacgcc ccgtcctggt aagcccgtca gggcgcggcc catccgggat acgcccttgt
    13861 gacgcatgtc actcgattcc tggtgatatg acgctgaccc aaaatgcgac taagcctaaa
    13921 gtgatcccca gccgcccgga gcccaccgtg cggcccaccg ccgccttact ccagaagggg
    13981 aaccgccatg gacactcgcc gccacagacg ccaccgctcc cgcctcgccc tcgccctggc
    14041 cggagccttt gccggttttg ccggactcac cccctccctg caggccgccc agttcgcggt
    14101 cggcaccttc gccccctggc cgggctacgc cattcccaat ggtcagtgga tgtcggccga
    14161 cgtaaacggt gacggcaagg ccgacatcat ccacgcggtg gccggcgccg attacgtgaa
    14221 cacctggctg tccaacggca atggcagcta caccgtcaag agcttccgtc cctggtccgg
    14281 ctacggcatc cccaacggcg tgtggctcac cggtgacatc aacggcgacg gcaaggccga
    14341 catcgtccat gccgtcgcca acactgatta cgtgcacacc tggatgtcca acggcgacgg
    14401 cacttacacc atgggcacct tccgcccgtg ggccggctac ggcattccca acggagaatg
    14461 gcgtattgcc gacgtcaatg gcgacggcaa ggccgacctc atccacgctg tggcaggcgc
    14521 cgattatgtg aatacctggc tctccaacgg caacggcacc ttcaccgtca agagcttccg
    14581 cccctgggcc ggctacggca tccccaatgg cgtgtggctc actgccgacc tcaacggcga
    14641 cggcaaggcc gacatcgtcc acgcggtggc caacaccgat tacgtacata cctggatgtc
    14701 ccgcggcgac ggcacctaca ccatgggcac cttccgcccc tgggccggct atggcattcc
    14761 caacggccag tggatgaccg gcgacgtgaa tggcgacggc aagaccgacc tcatccatgc
    14821 tgtcgcgggc gccgactacg taaacacctg gctgtccaac ggcagcggcg gcttcaccgt
    14881 acgcagcttc cgcccctggg ccggctacgg catccccaat ggcgaatggc gtgtcgccga
    14941 tctcgacggc gacggcaagg ccgacatctt ccacgctgtc gccaacacca gctacgccca
    15001 ccagtggctg tccaacggtg acggcaccta ccgcgtgcag accttctccc cgtgggccgg
    15061 ctacgccatc cccaacggcg tgtggctcac cggcgacttc aacggcgacc gcaagaccga
    15121 catcatccac gccgtcgcca acaccaacta cgtgcacacc tggcgttcca ccctgccacg
    15181 ccctggcgaa ctgtccctcg atggcctgga gatcacccag agcgtgcaga acatggccca
    15241 ggaggtcggc ctcgtcgccg acaaacgcac catcgcccgc gcctatgtga gcatcaacgg
    15301 tggcgcagcg gtcaccgtcc gcggcgtgct caccgccacc cgtacgggcg ccgcaccggt
    15361 cagcatcacc tccctcaaca cggtcaccct cgacccggca ctggctggca acctgcgtgc
    15421 caagcgcgag aacctggcca gcagcctcaa cttcgaagtg cctcccgccc tgcgcagcgc
    15481 cggtgccacc cagttccgga tcacggttca gcgtgtctcc ccgggcgcgg ccctcgtctg
    15541 cgcggactgc gacagcagcc cggtgggcgt cagctttgcc accgccgcac cactacgggt
    15601 ccgcgtgctg ggcctccgct acagcagcgg cacgcctgcc acggtcaaat ccccccgcgc
    15661 cctggactac accctggtcc gctcctggct cggccgcgcc taccccgtct ccaccctcac
    15721 catgagcacc gcgagcgtca atgccagcgc cgcctggccc ttcagctgca acgacgccaa
    15781 cgcccagctc gccacgcttc gcgccaacga cgtggccgcc ggcaccgaca agcgcaccca
    15841 ctacttcggg ctggtcgccg atgggggcgg cttcatgcgt ggctgcgctg ccagcatccc
    15901 tggcagcgcc gaccccaccg ccgtcgcctc cggccccacc ggcacgccga gcggcagctt
    15961 cggctgggac accgacggct cctacggcga ctggtacacc ggtcatgaac tgggccacac
    16021 ctacggccgc ctgcatcccg gctcgggctg cggcgactcc gccgacgaca gccacgaacc
    16081 cttcaccaac ggcgcaattt ccggccccga cggcgcctgg accggcctgg acgtgggcga
    16141 cactgccaac ggtattccca tggcggccct gcccggcgcc cagtggttcg acgtcatgac
    16201 ctactgccgc aaccagtgga tcagcttcta cacctacaac ggcatccgcg cccgcctcac
    16261 cggcgagaac gccctgcccg ccggtgcgcc ggcccccaag gcgctcaaga cctcagccgc
    16321 cctgccgccc atggcccagg tcggcgaacg gcccgagccc atcactggtg ctaaggcagc
    16381 gcccgtcctg gcggctccgc gtcgtgccgc gcccatgctg gccgcggcgc cggtcaccgg
    16441 ccacgcgcgg ccgccggcca tcattgacgg cacagcctcg cccgcgacga gtaccgcccc
    16501 cgcgtccgaa ccgcctgcag ccgcctatgc caccccaccg acacccgtga tcggttaccg
    16561 ggaagttgcc gcagcccccg ttgccaacgc ccgcgccgtg ggcgacgaca tcccgatgca
    16621 gcgcggcgac ctgctctcgg tggtggctac ggtcaatctc acccgccaga ccggccgcct
    16681 cgtctccatg agccgcattc ccactggcct ggccacaccc gtcggaccgg aagacgcccg
    16741 catcgagttc ctcgacgccc gcggcaaggt gctggccgcc tacgccgtgc ccttccggcg
    16801 cgactccgat gtggaagccg gcgccgacca gaccggcctg atcgacgccg tcgtgccctt
    16861 ctcgaccacg gccgccaagg tccgcgtcag cgccttcggc acgctgctgg gtgagcggga
    16921 catccccgcc aagccgccca ggctcggcaa gatcagcgtg ctcaagtcca aggccctgga
    16981 cgaaggcgcc accctgagct ggacggccgt tgccgggccc ctcgtcagct acagcgtgca
    17041 gagcagcgag gacggcggca agagctggca gaccctcgcc atcggcctgc gcgaaccgcg
    17101 cttccagctc ccgcccgcgg aaggtggcgt cacgccccgc taccgcgtca tcgcccacga
    17161 cggcatccac aacaccatcg tgccagtgcg ctgaacgact ggccgatctc cctctggcac
    17221 ctccctgttg ccgccgtgcc cacccgggcc ggcggctttt ttgcgccggg tgcgccgctg
    17281 cgcgggtaaa aaagcgtcga aatatcagtc gcttctaata ctcactggaa atctgttgcg
    17341 gcggggcgat tcttgcccac ggtcaatttt tttcgccccc ggccccccta gagtgccgct
    17401 gcgacaactt gtcgcaggga tgcccggacc cgcggcccgg accttcccgt tcgctaaggg
    17461 acacacggag accaacgatg agcaccgaac accatcctca agacgaccag gacctcgtac
    17521 cgggccgccg caaattcctg aacaccgccg ccctcgccgg tctcgccggc gccggcctgt
    17581 ccgtcggcct gtccgcctgc aagcaggagg ccgcgccgac caccgccccc gcagccccgg
    17641 cgcccgcggc caaggccgca gaaggcggtg agctgcccat ccacccggcc cccggccagc
    17701 tcgacagcta ttacggcgtg tggtccggcg gtcactccgg cgaagtccgc atcctcggcc
    17761 tgccctccgg ccgtgagatc aagcgcgtgc cggtcttcaa catcgactgc atgagcggct
    17821 ggggcatcac caacgaatcc aaggccgtca ttggcaccaa ggccgacggc agcctcaagt
    17881 acaagaccgg cgacacccac cacgtgcacg gctcgtacac cgacggcacc tacgacggca
    17941 aatacttctg ggtgaacgac aagatccacg gccgcctggc gcgtatcaag ggcgacacct
    18001 tcgagtgcga caagatcacc gagatcccga acatccaggg cttccacggc atcttcccgg
    18061 acaagcgcga cccggtcgat caggccatca accacaccac ccgcgtcttc tgcggcaacg
    18121 aattccacgt gccgcagccc aacgacgggc gcgacgagga cgacgccacc aagtaccact
    18181 gcctgttcac ctgcatcgac gcagaaacca tggaagtgcg ctggcagtgc cgggtggacg
    18241 gcaacatgga cctggtggcc tcgtccttcg acggcaagct ggccgcctcc aaccagtaca
    18301 acaccgaagg cggcgcccat tacgaggaca tgatgtccgc cgagcgcgac gcctgcgtgt
    18361 tcttccacat cgagcgcatc gaggccgcca tcaaggccgg caaggccagc accatcggcg
    18421 acagcaaggt gcccgtggtg gacggcaccc gcgccgccaa cccggatccg aagaccgccc
    18481 tcacgtgcta cgtgccggtg cccaagaacc cccacggcgt caatgccagc ccggacggca
    18541 agtacttcat ctgctccggc aagctgtccc ccaccgccac cgtcattgag ctgagccagg
    18601 tgctggccta cttcgacggc acgctggacg acctcaagaa ggccatcgtc gccgaagtgg
    18661 aactgggcct cggccccctg cacaccgcct tcgacggccg cggcaacggc ttcaccacgc
    18721 tcttcctgga ttcccaggtg gtcaagtgga acatcgacca ggccatcaag ttctacgccg
    18781 gcgacaagaa cgccaagtac gtggtggatc gcgtggacgt gcattaccaa cccggccaca
    18841 tcaacgcgtc catgtccgaa accaaggagg ccgacggcca gttcctcggc gtgggctgca
    18901 agttctccaa ggaccgcttc ctgcccgtcg gcccgctgca ccccgaaaac gagcagctca
    18961 tcgacatccg cggcgagaag atgaagctcc tggccgacca cccggtctat cccgagcccc
    19021 acgacttcat catcgtcaag cgcgacaaga tcaagacccg gcaggtctac aacgtggacg
    19081 acttccccaa cgccaccaag gacccgaaag attccggtgt cacccgcaac ggcaacaagg
    19141 tcaccgtgcg cctggtctcc caggccccgg cctacagcct gcgcgagttc aaggtgaaga
    19201 agggcgacga ggtcaccctc atcctcacca acctggacaa ggtggaagac ctcacccacg
    19261 gcttcgcgat tcccaagtac gacatcaact tcatcgtgaa cccgcaggaa accaagtccg
    19321 tcaccttcaa ggccgacaaa ccgggcgtgt actggtgcta ctgcacccac ttctgccacg
    19381 ccctgcacct ggagatgcgg acccgcatgt tcgtggagaa gtgacaccac gaccgctcga
    19441 ttgacgaaga gagcttgggg agacgtccgc gtctcccctt ttttgtgaac acgatccgcc
    19501 cccgtgcgaa tcgtcttcac gccccggtga aaggccttca ccgccctgcg cagacgatgt
    19561 gcacgcctgc gcagcgtgtt cgcccgcctg ccaatacact gagcagcacc gccaagacgc
    19621 tgagcactgc tgccaagatg attcgcaccg atgcgaagac gctgagcacg tctgctcaga
    19681 ccctgagcac gaccgctcag acactacgca ccgcggccaa gaccctgagc acgcacgctc
    19741 agaccttggg caccgcggcg aagacactga gcagccgtgc caacaggatg cgccggcccg
    19801 cgcaagacat tcaccgggcg catcagaaag tgcgtgttct cgccctgctc gaaacgccag
    19861 cccctcacac gctggcctca gacgcccctc gacacatccg gcatcggtca agcccgcgcg
    19921 cccgacgggc gatactgcgg ggacgatgac ggactcggca gtggccaggc caggcggagg
    19981 aatcatcccc gcagcatgcc cgccggcccg ccgcctgtgg caaattgact cagcttaagg
    20041 cggccacgcc ggcggattat cagaatcggc tgataaattc cccagccgga gccccccgac
    20101 gatgttctcc ctgcgaaccc tggccgccgc tcttgccacg gcagccgcct gcctgctggc
    20161 cggcgcggcc caggccgaca cctacgaagt caaactgccg ccggaactgt tcacctcgcc
    20221 gcgcctgtgc gactacgccc cctgcaccga tgtgctgccc ggcgccaccc gcttctccga
    20281 gcgcaagggc cagcccagct atgtggaagg ctacaaggac gtggacggca cgccccggct
    20341 cgccggctac gtgatgctgt ccaccgacat caccgacatc cccgcctatt ccggcaagcc
    20401 cgtcatcacc ctgatgggca tggacaccac cggacacttc accggcgtgc gcatcctcaa
    20461 gcactccgaa cccatcctcc tgctgggcat ccccgaagcc gccctggtgc gcttcaacaa
    20521 ccagtacctg ggcaagttcg tcggtgacaa catcgagatc ggcaaatccc gccccgagga
    20581 caacctcatc ggcctggacg ccatcaccgg cgccaccgtc accgtcatcg cccaga
//
LOCUS       DAOQPA010000057        20478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1272_length_20478_cov_18.415953, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000057 DAOQPA010000000
VERSION     DAOQPA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_20478_cov_18.415953"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /gene="nifK"
                     /locus_tag="PKX01_09500"
     CDS             <1..1113
                     /gene="nifK"
                     /locus_tag="PKX01_09500"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HMZ76402.1"
                     /translation="MAEVIGDDLNAFINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGW
                     DNMEEGILRYFTLNNMEGKKAGTKQTINLVPGFETYLGNYRVVKRMLTEMGVGYTMLS
                     DPTDVFDTPADGEFRMYAGGTTMDEVKDAPNAITTVLLQPWQLEKTKKFVETTWAHDV
                     PKLNIPMGVEWTDEFLMKVSELTGQAIPASLELERGRCMDLISDSHAWLHGKKFALYG
                     DPDFVMGMVKILLEVGAEPTHILSHNANKRWGKAVQKLLDASPFGKLGKVYVGNDLWH
                     MRSLCFTDKPDFLIGNSYGKYIQRDTRHKGEEFEVPLIRIGFPIFDRHHMHRATTLGY
                     EGMMSVVTQLTNAVLEQLDKETIGMGTTDYNFDLVR"
     gene            1171..1395
                     /gene="nifT"
                     /locus_tag="PKX01_09505"
     CDS             1171..1395
                     /gene="nifT"
                     /locus_tag="PKX01_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HMZ76403.1"
                     /translation="MANIMIRQGEKGLTFYLPKRDLEASIESIEFDSPEKWGGELKLD
                     NGGSYYIDPIAKPARLPISLRAKRLGAAED"
     gene            1412..1603
                     /locus_tag="PKX01_09510"
     CDS             1412..1603
                     /locus_tag="PKX01_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ76404.1"
                     /translation="MAMKIDPDMCTACGDCKPVCPTKSISSGKIFYKIDASTCTECDG
                     HNDSPLCVDVCPSGCISPA"
     gene            1670..2395
                     /locus_tag="PKX01_09515"
     CDS             1670..2395
                     /locus_tag="PKX01_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HMZ76405.1"
                     /translation="MSDSPIASREAALRIALAARCLAGLDVRAFVGALIDKLEAPLTE
                     AKLGTLTVEDLRALLAGDFAEQGCHVGVDADTLKEAIRLLWGQGVGAGDFPEVEAYSD
                     GDMPGSIRVAIAANRGENLDGHFGSCERFLIYQVGLDAIRLVGVRSTVEADAAEDRNA
                     ARSELINDCQIVYVQSIGGPAAAKVVRAGVHPVKKPTGGPAREILTELQGRLSSPPPW
                     LAQIMGVPAASLARFEEELEAED"
     gene            2398..2661
                     /locus_tag="PKX01_09520"
     CDS             2398..2661
                     /locus_tag="PKX01_09520"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HMZ76406.1"
                     /translation="MITPEQLTLVADAVRADAAIASLRARFPDLHFTECSEDDVSPRY
                     RAALETDGHALYYISGATGHCLEVTNDAALATGILVAAKADEA"
     gene            2651..3412
                     /locus_tag="PKX01_09525"
     CDS             2651..3412
                     /locus_tag="PKX01_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HMZ76407.1"
                     /translation="MKREDKGAADAGIGRLGLCSGCSYFPDFLNAGRCVPGDACIRAH
                     SGRQIDRFLRANPELSGAYLADLFWERRAIAARYAPVEAIRGLRSDPDEVVRRVVATR
                     IPADELDSMLGDPDREVRLSVASRLPEPLLTRMVQDPDYLIRATVARRLPHGQLSRLA
                     KDAEREVRKVVASRLPPFALHMLADDPEPEVRAIVAERALPGLAAALLQDSEWWVRLA
                     AVGNAPLEFLPPLTSDPELEVREAALARLADSQAA"
     gene            3431..4405
                     /locus_tag="PKX01_09530"
     CDS             3431..4405
                     /locus_tag="PKX01_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76408.1"
                     /translation="MKDPQAWPEYCKSLAPAIVETCTHTAVNTGPAGLVAALGSVVPE
                     WKFRHAFSRGGWYRLGGVYDGAGERVSDSLEAWVDAELDARGGELESLLDDYAGQAHY
                     ASRLVGQTHYLVAPSGEGAGDFLQLEIECLQEVRAHALFATTPDSVEDLVDPPRAAGS
                     AALPPIGLPTYTFRRLQHVGDFLRRMLVQKPEPAPIHRLLTDWDRSSAGIASAFSNHW
                     VILTQEHQDRYQQPVLHARPLATLAGHPPEFSAGESTRGVALGDALIRFNRAVGYPMA
                     WYFHMLTTKAVPYWVAQAVVEDALAGFAYLPEKDVAVVRDWLHRPYSV"
     gene            4822..5961
                     /gene="nifV"
                     /locus_tag="PKX01_09535"
     CDS             4822..5961
                     /gene="nifV"
                     /locus_tag="PKX01_09535"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HMZ76409.1"
                     /translation="MPQTSFVTINDTTLRDGEQSAGVAFSLDEKLEIARQLAAVGVPE
                     LEVGIPAMGAEERDSIRAVAALDLAPRLMVWSRMHPDDIAACADLGAHLVDISVPASD
                     QHLAYKLKQDRAWLLRELPRYIGAALSLGLEVGVGCEDASRADLDFLCALAETAERAG
                     ARRLRFADTLGILDPFGTYERISTLRRATDLEIEIHAHDDLGLATANTLAACLAGATH
                     ANTTVNGLGERAGNAPLEEVVLGLRKLHNIETGIDLKGFPALSAQVASASGRAVPWQK
                     SVVGPGAFCHEAGIHVDGLLKHPDNYQGFDPREVGRSHRIVLGKHSGSRAVQVVFAEL
                     GIPLDEREAQALLTRVRAFVADHKHPPEAADLRRLLASERSHFHA"
     gene            5954..6724
                     /gene="cysE"
                     /locus_tag="PKX01_09540"
     CDS             5954..6724
                     /gene="cysE"
                     /locus_tag="PKX01_09540"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HMZ76410.1"
                     /translation="MPDAMTIIAPTPAQPGLLDLLKEDLACVFQRDPAARSTLEVLTT
                     YPGVHAILLHRVSHRLWRAGWRYPARFLSFLSRTLTNVDIHPGATIGHRFFIDHGACV
                     VIGETAEVGDDVTLYHGVTLGGTSWNKGKRHPTLASGVVVGAGAKILGPIVIGERVRV
                     GANSVVVKDVPADRTVVGVPGRIVDTRHARSEDGVDLNHNVLPDPVAKSIACLIERIE
                     TLEKELAELRHEAPPPDHSGQCQTCSAGDLCCEHESMH"
     gene            6732..7256
                     /locus_tag="PKX01_09545"
     CDS             6732..7256
                     /locus_tag="PKX01_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76411.1"
                     /translation="MDLLDFSECKLYFEEDLPPEAEEMLAQASREYGQPDAEIALLRA
                     NLMVPDNLTVLVGLYRFYFYQHRLEEALRVAERAMYLSGRQLSLPDWESLDESSLAIA
                     AAISFGLLRFYLLALKAASVVLLRLGRIEESRARLHVLAAVDSRDQLGASKLLEVIAT
                     FENPDESDLSLVAA"
     gene            7267..7608
                     /locus_tag="PKX01_09550"
     CDS             7267..7608
                     /locus_tag="PKX01_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HMZ76412.1"
                     /translation="MDDTLTLDEAMEELVSAEDFLNYFGVPYDQSVVHVNRLHILQRF
                     HDYLAKQAKEMPEDEAARTDIYRQWLTRAYTDFVNSDALTEKVFAVFQNTSTPEGGMT
                     SFVSIDKVFQS"
     gene            7605..8075
                     /locus_tag="PKX01_09555"
     CDS             7605..8075
                     /locus_tag="PKX01_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HMZ76413.1"
                     /translation="MMDARWEYGEAVRLTRNVRNDGTYPGLDPGAFLVRRGSIGYVVD
                     VGTFLQDQVIYSVNFLDEGKIVGCREEELIGADEPWTPSRFEFRDKVRAVKGLSVGGE
                     MLITAGATGEVIKVIRDAPGGVAYHIHFDSLPGRILQIPESLLESATAEAPAEE"
     gene            8081..8893
                     /gene="nifM"
                     /locus_tag="PKX01_09560"
     CDS             8081..8893
                     /gene="nifM"
                     /locus_tag="PKX01_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HMZ76414.1"
                     /translation="MLHGYLELKLAWEMFQKAPDALSEPERIRVGDIARKQDRIELRI
                     LASAEAAQVAIPAATLRQRVDEIRGRYPSEDDLIRDLERIGLSEGELDKAVERDLRVE
                     GVLEKVASAAEPVSAVDAEIYYRLHPEAFDRPEARRLRHILITFNGGNEKARAHKTLD
                     GLRGLGSAEAFAKAALRHSQCPTAMDGGLLGVVKRKQLYPELEPAAFALALGAVSEVL
                     ESPIGLHILRCDEIFPSGLMPFEEVCPRIIERLADKRRQEAQRAWIKSLPAA"
     gene            9047..11185
                     /locus_tag="PKX01_09565"
     CDS             9047..11185
                     /locus_tag="PKX01_09565"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HMZ76415.1"
                     /translation="MNKKLTWSLAAIAFATAAAALETATPPNRLAPTQDHAQTAYLSS
                     QLLARYHYKAVPLDDALSEKIFERYFKSLDPERLFFTQADIDAFADARTTLDDAILQG
                     NLGVPFAIFQRFEQRVLERLTQAREVLAGSFDFNVKESYGYSRDKAPWPKDDAEARDL
                     WRKRVKNDWLRLRLAGKDDKAIRETLEKRYDNSLARAGRNKADDVFQIFMNAYAMSIE
                     PHTNYLGRAASEDFDIGMRLSLMGIGAMLQERDEYITIRELVPGGPAARSGQLAPGDR
                     IVGVGQGESGALVDLLGTRVDEAVKLIRGEKDTKVRLDILPADAGPDGRHKQVVLVRD
                     KIKLEEQAAKKSILKVGEGAGARKVGVITLPAFYEDFEGRRRGDKDYRSASRDVARLL
                     DELRKDKVDGVLVDLRNNGGGSLREAVELTGLFIDKGPVVQQRRADGRIGVESDERAG
                     VAWDGPMGVLINRGSASASEIFAAALQDYGRAAILGEPSFGKGTVQTLVNLDEVVRND
                     KPRFGELKMTVAQFFRVNGGTTQLRGVTPDIRLPSVSDDESYGESSFDNALPWVKIDA
                     APFTPAGNVVPLVPDLQRRHQARVARDKDFQYLLEDVAEFRRLRDSKQISLNEAERRK
                     ERDTQEARLKAREKTGTKDKLANLRDDGLQAGERSLAADLAAEKARKERKDVLLQEAA
                     SIVGDEVDLQASATPRLALKERRPGQPVIR"
     gene            11366..12523
                     /locus_tag="PKX01_09570"
     CDS             11366..12523
                     /locus_tag="PKX01_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76416.1"
                     /translation="MITGKGAFCALMFSAGILSAPAATADEGPRLSGSPKVEFTDSTA
                     ERIGQTRRVAITNVILEFQTKVFAEHITGRLSKMYLKRGDSFSDNELAGFDPRQLEQA
                     ANQVYKKLVTDLTAAGFEVVPEAQVLAQPAYQKLRETMGWPQGYHFGNKEGHSLIVGP
                     TALKPYVPPLSEQGTFANTRQEKGELQAPALGWSDRARFAGNSSYAAGLEVQLAKALN
                     AHVVKVWYLIGFGNATASTDWDAISTAHFSTTATSRTGATAQMYLRDEETRLAVRLPD
                     GDAGYARESKRINAITGGYRPYDGDVVVRLDETIPGEADFLAGGGVQNATTERDAKAG
                     IGGLLGSMAKSLTGTIADNSYKTTVDPTRFAAAAADMAGQLQPFLIARIKR"
     gene            12744..13886
                     /locus_tag="PKX01_09575"
     CDS             12744..13886
                     /locus_tag="PKX01_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76417.1"
                     /translation="MLHLRQLARATALGVLSLPLPALALEAGGLDFTGTGFMTLAAGR
                     VLSGTHDASVDQGFRCPCFISDYAQRGVYESGQWRAGPDSRLGLQGTVSAAQGRFALT
                     GQAVARGAANGAADLEWLYATAELSGNLTAQIGRKRLPLFSYSEAQDVGFALPWVHLP
                     PQLYGWEIVNYDGASLVWREQWGAWGSAVQVFGGNETRHDSGYWQIYNGKFSRTDVRW
                     SDILGTEVTLSRDWLTLRAVRIQSYTQNKVVSLGETAFSDRKRQVIQGLSMGVDSGNW
                     FARGEFLYINRTQDYGKDYANLIAVGYRHGPWQPLLSRSDYRQQLDTAGVPERHGTWS
                     AVLRYDLTEESALKVQADVWTDKTEPGFGSLHGDARLLTISYDRVF"
     gene            13886..14338
                     /locus_tag="PKX01_09580"
     CDS             13886..14338
                     /locus_tag="PKX01_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76418.1"
                     /translation="MSLPFSSSRVRQSLCHAATLLVLLGAPFSVRSADEMVVIINPEN
                     PNVIDRAYVVKVYTGAVKGWPDGSPIFLLDYGQDSELRKTFYTAVIGKSPAAMRAIWS
                     QNIFTGKAMPPRVTQEDSEMKHIVASNRNAIGYIRASQVDKTVKVIER"
     gene            14345..16621
                     /locus_tag="PKX01_09585"
     CDS             14345..16621
                     /locus_tag="PKX01_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76419.1"
                     /translation="MNTAGPARTRVWQNEARLVPVITWIATVTALLVAIAAPTGYFLL
                     AREAELRDAAMAARLHAAFVTQLIAASPDAWHQEVGGLLDTDLAPSDVPEQRRIVDAA
                     QQPIATTGAPLGRLVVERAAALRGPDGAVGEVIITRSLRPTLVRTGLVALLGVALGFT
                     IYGSLRVLPLRALRRAVAALQREESKAREEAEEQLRVVFQNAVEGIALFTPDRRITSC
                     NPAVARMFGRPLEHLPGMFITDLLRPFSGEEQGTAFTTGQGETTAFRASGEAFPVEMS
                     VNEARLADRPHFIAILRDITERKQAERRLSYLANYDSLTGLPNRSLFRERLERAMGRT
                     RRNGRQLALMFLDLDRFKNINDTLGHEAGDQLLRHVANALIANLRSTDTVSRATLDES
                     TESITVSRLGGDEFTVLVEDITSPAAAAQVATRILAAIAQPFEIHGERLYISTSIGVT
                     LYPHEAEDLDGLVKQADMAMYRAKELGRNTYFFYNDELNTEMAERHALEASLRHALER
                     REFHLHYQPKACLRSGRVTGVEALLRWTPSSGQRIGPDRFIPILEDIGLINQVGTWVL
                     RTACAQIAAWKTAGLPPLRLAVNVSARQFRQPNLPEQIAGLLAEHGLAPADLEVELTE
                     SMLMEDSEQSRDILSRLGTMGIQLAIDDFGTGHSSLAYLKRFNVHALKIDRSFVKDTP
                     EDPEDSAIATAIIGLSANLKIRVVAEGVETQAQADFLQQQGCDEMQGYLLSAPLPPRE
                     FAIWLRQRTAAGAVDPVT"
     gene            16688..17287
                     /locus_tag="PKX01_09590"
     CDS             16688..17287
                     /locus_tag="PKX01_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HMZ76420.1"
                     /translation="MENPQSLVHFWFGTDPDDAVTAARQAGLWWGKDGAVDAEMARRY
                     GHLPDDALAGRLDGWAATPAGRLALILVTDQFPRNIFRGTAQAFAFDATARRLCREGL
                     AHGQDRALRPIERVFFYLPLEHSEDADDQALSVTLFEALAAEVAVPLQPIFDGYADYA
                     RRHRDIVARWGRFPHRNAVLGRASTAAEDAFLQEPGSSF"
     gene            complement(17284..18021)
                     /locus_tag="PKX01_09595"
     CDS             complement(17284..18021)
                     /locus_tag="PKX01_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HMZ76421.1"
                     /translation="MTYCVAMRLDAGLVFLSDSRTNAGVDQINTFRKMQVFEKPGERM
                     LVLLSAGNLSISQSVVNLLRERLEQSRGPNLHTVKNMFEAARHVGDCLREIHARDAQA
                     LRDFGIDFNASLILGGQIAGEEPRLFQIYAVGNFIEATRETPYFQIGESKYGKPILDR
                     VITSETPIDQAAKCALISMDSTIKSNLTVGLPLDMLIYERDALKVGKQVTITAQNTYF
                     NDLRNRWGECLRNAFEELPAPDWDASF"
     gene            complement(18018..18977)
                     /locus_tag="PKX01_09600"
     CDS             complement(18018..18977)
                     /locus_tag="PKX01_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HMZ76422.1"
                     /translation="MLSRTADHLFWMARYIERAENLARLLDVTWQNSLVPQAPAAASE
                     NWRAVIALNSLEAAYSAKHGNETTADKVLHFMVSDPANPASIFCCLRAARENAHAVRG
                     TITSEMWETLNATWIELRGQSHEQRMANGPGEFFDWVKMRASLNRGTTSGTMLQDEAY
                     NFIRLGTLLERADNTARILDVKFHVLLPVNHDSEDAITDFYQWGALLRSVSGFEVYRK
                     VYRDVITPERVAELLVLHPDMPRSLHFCLRGVVKNLDLLANSHSGETQRRAGMLYARL
                     RYGCVEDFLAGGLHEFLSDFMDRIYEIGAGINRDFLAPVEGLQ"
     gene            complement(18982..20403)
                     /locus_tag="PKX01_09605"
     CDS             complement(18982..20403)
                     /locus_tag="PKX01_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HMZ76423.1"
                     /translation="MNFYNEMHDGAGGVRAHYERYAAWLASSPAERIQRKRAEADLAF
                     RKVGITFAVYGDASDGDGGKERLIPFDIIPRVIPAPEWRDLSAGLRQRVQTLNAFLHD
                     IYHDQEILKAGVIPAEQVLNNAQFRPEMMGVDVVGNIYAHIAGVDVVRAGKGEFYVLE
                     DNLRVPSGVSYMLEDRKMMMRLFPELFSIHKVAPVQHYPDLLLEKLRAVAPKGISDPT
                     VVVLTPGAYNSAYFEHTFLAQQMGVELVEGRDLFVEDDTVYMRTTQGRRRVDVIYRRV
                     DDDFLDPTVFRPDSMLGVRGLLAAYKAGNVTLANAIGTGVADDKSIYPYVPDMVRFYL
                     GEEPKLNNVPTYQCRKPEDLAYTLAHLPELVVKEVHGAGGYGMLVGPAASTAEIERFR
                     GLLTAHPARYIAQPTLALSNCPTFVESGVAPRHLDLRPFVLSGKDVDMVPGGLTRVAL
                     TEGSLVVNSSQGGGTKDTWVLEE"
BASE COUNT         3553 a         7463 c         6281 g         3181 t
ORIGIN      
        1 atggccgagg tgatcggcga cgacctgaac gccttcatca acaacgccaa gaaggccggt
       61 cacgttcccc aggaataccc ggttcccttc gcccacaccc cctccttcgt cggctcccac
      121 acgaccggct gggacaacat ggaagaaggc atcctgcgct acttcaccct caacaacatg
      181 gagggcaaga aggccggcac caagcagacc atcaacctgg tgccgggttt cgagacctac
      241 ctgggcaact accgcgtcgt gaagcgcatg ctcaccgaaa tgggcgtcgg ctacaccatg
      301 ctgtccgatc cgaccgacgt gttcgacacc ccggccgacg gtgaattccg catgtacgcg
      361 ggcggcacca ccatggacga ggtcaaggac gcgccgaacg cgatcaccac cgtcctgctc
      421 cagccctggc agctcgaaaa gaccaagaag ttcgtcgaaa ccacctgggc ccacgacgtg
      481 cccaagctca acatccccat gggcgtcgag tggaccgacg agttcctgat gaaggtctcc
      541 gagctgaccg gccaggccat ccccgcctcg ctggaactgg aacgcggccg ctgcatggac
      601 ctgatctccg acagccacgc ctggctgcac ggcaagaagt tcgccctcta cggcgacccg
      661 gacttcgtca tgggcatggt caagatcctg ctggaagtgg gtgccgaacc gacccacatc
      721 ctgtcccaca acgccaacaa gcgctggggc aaggccgtgc agaagctgct ggacgccagc
      781 cccttcggca agctcggcaa ggtctacgtc ggcaacgacc tgtggcacat gcgcagcctg
      841 tgcttcaccg acaagcccga cttcctgatc ggcaactcct acggcaagta catccagcgc
      901 gacacccgtc acaagggtga agagtttgaa gtgccgctga tccggatcgg cttcccgatc
      961 ttcgaccgtc accacatgca ccgggccacc accctgggct acgaaggcat gatgtccgtg
     1021 gtcacccagc tcaccaatgc ggtccttgag cagctcgaca aggaaaccat cggcatgggc
     1081 accaccgact acaacttcga cctggtccgc taatcacggc cagaccggcg gggccgcaag
     1141 gccccgccgt ttccgcagga gcgttcgtaa atggccaaca tcatgatccg tcagggcgag
     1201 aaggggctca ccttctatct cccgaagcgc gacctggaag cctccatcga gtccatcgaa
     1261 ttcgacagcc cggaaaaatg gggtggcgaa ctgaagctgg acaatggcgg ctcgtactac
     1321 atcgacccca tcgccaaacc cgcgcgcctg cccatctccc tgcgtgccaa acgtctcggc
     1381 gccgccgagg actgatctgg aggacttcat catggcaatg aaaatcgatc ccgacatgtg
     1441 taccgcctgc ggcgactgca agcccgtctg cccgaccaag tcgatcagct cgggcaagat
     1501 cttttacaag atcgacgcca gcacctgtac cgagtgtgac ggccacaacg actcgcccct
     1561 gtgcgtagac gtctgcccgt ccggctgcat cagcccggcc tgaggcaagc ccagtgccga
     1621 gcgccggccc cggccggcgt tccactctgg actttcctgg agccctgtca tgtctgactc
     1681 ccccatcgcc tcccgcgagg ccgccctgcg catcgccctg gcggcccgct gtctggccgg
     1741 ccttgatgta cgcgccttcg tgggcgccct catcgacaag ctcgaagccc ccctcaccga
     1801 agccaagctc ggcaccctca ccgtcgagga cctgcgggcg ctcctggcag gcgatttcgc
     1861 cgaacagggc tgccacgtag gcgtggatgc cgacaccctc aaggaggcga tccgcctcct
     1921 gtggggccag ggcgtgggcg ccggcgactt tcccgaggtc gaagcctatt ccgacggtga
     1981 catgcccggc tccatccggg tagccatcgc cgccaatcgg ggcgagaatc tggacggaca
     2041 ctttggctcc tgcgagcgct tcctgatcta ccaggtcggc ctcgatgcga tccgcctcgt
     2101 cggcgtgcgc agcaccgtcg aggccgatgc ggccgaggac cgcaacgccg cccgctccga
     2161 actcatcaac gactgtcaga tcgtgtacgt ccagtccatc ggtggtcccg ccgccgccaa
     2221 ggtcgtgcgg gccggcgtcc atccggtcaa gaagcccact ggcggcccgg cgcgggagat
     2281 cctgaccgaa ctgcagggac gtctcagcag cccgccgcct tggctggccc agatcatggg
     2341 cgtgccggcc gcgtcgctgg cacgtttcga agaggaactc gaagcggagg actgatcgtg
     2401 atcaccccgg agcaactcac cctggtggcc gacgccgtgc gcgccgacgc cgccatcgcc
     2461 agcctgcgtg cccgttttcc cgacctgcat ttcaccgaat gcagcgagga cgacgtgtcg
     2521 ccccgctacc gcgctgccct cgaaaccgac ggccacgccc tctactacat ctccggcgct
     2581 accggacact gcctcgaagt gaccaatgat gccgcccttg ccaccggcat cctggttgcc
     2641 gccaaggccg atgaagcgtg aggacaaggg cgcggccgac gccggcatcg gtcgcctggg
     2701 cctgtgttcg ggctgcagct acttcccgga cttcctgaat gccggccgtt gcgtgccggg
     2761 ggacgcctgc atccgcgccc atagcggccg ccagatcgac cgcttcctgc gggccaaccc
     2821 ggagctctcc ggcgcctacc tggccgacct gttctgggaa cggcgcgcca tcgccgcgcg
     2881 ctacgcaccg gtcgaggcca tccgcggcct gcgcagcgac ccggacgaag tcgtccgccg
     2941 cgtcgtggcc acgcgcattc ccgccgacga actggactcg atgctgggcg acccggaccg
     3001 ggaagtccgc ctgtcggtcg cctcgcgcct ccccgagccg ctcctgaccc gcatggtcca
     3061 ggacccggac tacctcatcc gcgccaccgt cgcccgccgc ctgccccacg gccagctctc
     3121 caggctggcc aaggacgccg agcgggaggt ccgcaaggtg gttgcatcgc gcctgccgcc
     3181 cttcgccctg cacatgctcg ccgacgatcc cgagccggaa gtccgcgcca tcgtcgccga
     3241 acgtgcgctg cccggcctcg cggccgccct gctgcaggac agcgaatggt gggttcgtct
     3301 ggccgccgtg ggtaatgcgc ccctcgaatt tctgccgccg ctcacttcgg atcccgaact
     3361 ggaggtccgg gaagcggctc ttgcccgttt ggcggattcc caagccgcct gatccctcga
     3421 ggaaattgcc atgaaagacc cccaggcctg gcccgagtac tgcaagtccc tcgcccccgc
     3481 catcgtcgaa acctgcaccc acacggccgt caacaccggt ccggcgggcc tcgtcgccgc
     3541 ccttggcagc gtcgttcccg aatggaagtt ccgtcacgcc ttctcgcgcg gcggctggta
     3601 tcgcctgggg ggcgtctacg atggcgccgg cgagcgcgtg agcgattcgc tggaagcctg
     3661 ggtcgatgcg gaactggacg cccgcggtgg cgagcttgaa tccctcctcg acgactacgc
     3721 cggccaggcc cattacgcat cgcgcctggt cggtcagacc cactacctcg tggccccctc
     3781 cggcgaaggc gccggagact ttcttcagct cgagatcgaa tgcctgcagg aggtccgcgc
     3841 ccacgcgctc ttcgccacca cgccggacag cgtcgaggac ctggtcgacc cgccccgcgc
     3901 cgcgggcagc gccgccttgc cgcccatcgg gctgcccacc tataccttcc gccggctcca
     3961 gcacgtaggt gacttcctgc gccgcatgct tgtccagaag cccgaaccgg caccgatcca
     4021 tcgactgctg accgactggg accggagcag cgccggaatc gccagcgctt tcagcaacca
     4081 ctgggtgatc ctcacccagg aacaccagga ccgttaccag cagccggtcc tgcacgcccg
     4141 gcccctcgcc accctggccg gccacccgcc ggagttcagc gccggcgaga gcacccgtgg
     4201 cgtcgccctg ggggacgccc tgatccgctt caaccgcgcc gtcggctatc ccatggcctg
     4261 gtacttccac atgctcacca ccaaggccgt cccctactgg gttgcccagg cggtggtgga
     4321 ggacgccctc gccggcttcg cctatctgcc cgaaaaggac gtggccgtcg tccgggactg
     4381 gctgcatcgg ccctattcgg tctgaacccg cgccggcatc ggccggccgc cctgggtgcc
     4441 gtaacgcccg acggcaccca gggcggccct gcgggtgtct gaggggcgga gcacggcccc
     4501 ccgcaccggg atgagcgccc atcccgttcc acagtacgtc cgcctcaccg cgcaggcccc
     4561 tgccccggat tcctagcgcc caccgcaccg ggtacgccca tcggacagcc atccacaacc
     4621 gtgtcacgcc ggtggggccc cttgcgggca ccggaccgtc tgtctgccct gcaggcaaga
     4681 cgcccctacg gtgttcgagc gccattgtcg ggttctcgac attgaacccc acagcccgcc
     4741 acacatttcc gcattttttc agtgaatcaa gcacttgaaa gctggcaccg tccttgctga
     4801 agccatggca aggagattgc catgccccag acttccttcg tcacgatcaa cgacaccacc
     4861 ctgcgcgacg gcgagcagtc ggccggggtc gcgttctcgc tggacgagaa gctggaaatc
     4921 gcccgccagc tggccgccgt cggcgtgccc gaactggaag tcggcatccc cgccatgggc
     4981 gccgaggagc gcgactccat ccgcgccgtc gctgccctgg atctggcacc gcgcctgatg
     5041 gtgtggagcc gcatgcaccc ggacgacatc gccgcctgcg ccgacctggg cgcccatctg
     5101 gtggacatct ccgtacccgc ctccgaccag catctggcct acaagctcaa gcaggaccgg
     5161 gcctggctgc tgcgggagct gccccgctac atcggcgccg ccctgagcct cggcctggaa
     5221 gtgggcgtcg gctgcgagga cgcctcccgc gccgacctgg atttcctctg tgccctggcc
     5281 gagacggccg aaagagccgg cgcccgccgc ctgcgctttg ccgatacgct gggcatcctc
     5341 gaccccttcg gcacctacga gcgcatctcc accctgcgcc gggccaccga tctggagatc
     5401 gagatccacg cccacgacga cctgggtctc gccaccgcca acaccctcgc cgcctgcctg
     5461 gccggcgcca cccacgccaa caccacggtg aacggcctcg gcgagcgcgc cggcaacgcc
     5521 ccgctggagg aagtggtgct gggcctgcgc aagctccaca acatcgagac cggcatcgac
     5581 ctcaaaggct tccccgccct ctccgcccag gtggccagtg cctccggccg cgccgtaccc
     5641 tggcagaaga gcgtggtggg acccggcgcc ttctgccacg aggccggcat ccacgtggac
     5701 ggcctgctca agcatccgga caactaccag ggcttcgacc cccgcgaggt gggccgctcc
     5761 caccgcatcg tcctcggcaa gcactctggc tcgcgcgccg tgcaggtggt ctttgccgag
     5821 ctgggcatcc ccctggacga gcgcgaagcc caggccctgc tcacccgcgt gcgggccttc
     5881 gtggccgatc acaagcaccc ccccgaagcg gccgacctgc gccgcctgct ggcctccgaa
     5941 aggagtcact tccatgcctg acgccatgac catcatcgct ccgacgccgg cccagccggg
     6001 cctcctggat ctcctgaagg aagacctggc ctgcgtcttc cagcgcgatc cggccgcccg
     6061 ctccacgctg gaagtgctca ccacctaccc cggcgtgcac gccatcctgc tgcaccgcgt
     6121 gtcgcaccgc ctgtggcgtg ccggctggcg ctacccggcg cgcttcctgt cctttctcag
     6181 ccgtacgctg acgaacgtgg acatccaccc cggcgccacc attggccacc gcttcttcat
     6241 cgaccacggc gcctgcgtgg tgatcggcga gaccgccgag gtgggcgacg acgtcaccct
     6301 gtaccacggc gtgaccctgg gcggcacctc atggaacaag ggcaagcgtc accccaccct
     6361 ggccagcggc gtggtggtgg gggccggcgc caagatcctc ggccccatcg tcatcggcga
     6421 acgcgtgcgc gtcggcgcca actcggtggt ggtgaaggac gtcccggccg accgcaccgt
     6481 ggtgggcgtc cccggccgca tcgtcgatac ccgccatgcc cgcagcgagg acggcgtgga
     6541 tctgaatcac aacgtgctgc ccgatccggt ggccaagtcc atcgcctgcc tgatcgaacg
     6601 catcgaaacc ctggagaagg aactggccga actgcgccac gaggcgcctc cccccgatca
     6661 cagcggacag tgccagacct gttccgccgg cgacttgtgc tgcgagcacg agtccatgca
     6721 ctgaggtctc catggatctg ctcgactttt ccgagtgcaa gctctacttc gaggaggatc
     6781 tgcctcccga agccgaagag atgctcgccc aggcctcccg cgaatacggc cagcccgacg
     6841 cggagattgc cctgctgcgg gccaacctga tggtgccgga caacctgacc gtccttgtcg
     6901 gcctgtaccg cttctacttc taccagcacc gcctggagga agccctgcgg gtcgccgagc
     6961 gggccatgta cctctcgggc cgtcagctca gcctcccgga ctgggaatcc ctcgacgaat
     7021 cctcgctggc catcgccgcg gcgatctcct tcggcctgct gcgcttctac ctgctggccc
     7081 tcaaggccgc cagcgtggtg ctgctgcgcc tgggacgcat cgaggaatcc cgcgcccgcc
     7141 tgcacgtact ggccgcggtg gatagccgcg accagctcgg cgccagcaag ctgctggaag
     7201 tcattgcaac gttcgaaaac cccgacgaat ccgatctatc ccttgtcgca gcctgaggag
     7261 gcccccatgg acgataccct gaccctcgac gaagccatgg aagaactggt gtccgccgaa
     7321 gatttcctga actacttcgg cgtgccctac gaccagtccg tcgtgcatgt gaaccgcctg
     7381 cacatcctgc agcgcttcca cgactacctg gccaagcagg ccaaggagat gcccgaagac
     7441 gaggccgccc gtaccgacat ctatcgccag tggctgaccc gcgcctacac cgacttcgtg
     7501 aactccgacg ccctcaccga gaaggtcttc gcggtgttcc agaacaccag cacgccggaa
     7561 ggcggcatga cctccttcgt gtccatcgac aaggtcttcc agtcatgatg gacgcccgct
     7621 gggagtacgg cgaagccgtc cgcctgaccc gcaacgtgcg caacgacggc acctatccgg
     7681 gcctcgaccc cggcgccttc ctggtgcgcc ggggcagcat cggctacgtg gtggacgtgg
     7741 gcaccttcct ccaggaccag gtcatctact cggtgaactt cctggacgaa ggcaagatcg
     7801 tcggctgccg cgaggaagag ctcatcggcg ccgacgagcc ctggaccccg tcgcgcttcg
     7861 agttccgcga caaggtccgg gcggtcaagg gcctctccgt cggcggcgag atgctcatca
     7921 cggccggcgc caccggcgaa gtgatcaagg tgatccgcga cgcccccggc ggcgtggcct
     7981 accacatcca cttcgacagc ctgcccggcc gcatcctgca gattcccgaa tccctgctcg
     8041 aatccgcaac ggcggaggcc cccgcggagg aatgaacccc atgctgcacg gctatctcga
     8101 actgaaactc gcctgggaga tgttccagaa ggcgcccgac gccctgtccg agcccgagcg
     8161 gatccgcgtc ggcgacatcg cccgcaagca ggaccgcatc gagctgcgca tcctcgccag
     8221 tgccgaagcg gctcaggtcg ccatcccggc agccaccctg cgccagcgcg tggacgagat
     8281 cagaggccgc tatcccagcg aggacgacct catccgcgat ctggagcgca tcggcctctc
     8341 cgagggtgaa ctggacaagg ccgtcgagcg tgacctgcgg gtggaaggtg tcctcgagaa
     8401 ggtggcctcc gccgccgagc cggtcagcgc ggtggatgcc gaaatctact accgcctcca
     8461 ccccgaagcc ttcgatcgac ccgaggcacg ccgcctgcgc cacatcctca tcactttcaa
     8521 cggcggtaac gagaaggccc gcgcccacaa gaccctggac ggcctgcgcg gcctgggcag
     8581 cgccgaagcc tttgccaagg cggccctgcg ccactcccag tgccccaccg ccatggacgg
     8641 cggactgctc ggcgtggtca agcgcaagca gctctacccg gagctcgaac ccgccgcctt
     8701 cgccctcgcc ctcggcgccg tgtcggaggt gctcgaatcc cccatcggcc tgcacatcct
     8761 gcgctgcgac gagatcttcc cgagcggcct catgcccttc gaagaggtct gcccgcggat
     8821 catcgagcgc ctcgccgaca agcgtcgcca ggaagcccag cgcgcctgga tcaagtccct
     8881 gcccgcggcc tgaccccgcg gcggcgcaaa ccgcccgtcc gtcgccgcgg gtggcccatt
     8941 gttcacgaac atgaaagaat gctccacgac ggccgataaa gcggtctgat gacgcgcacc
     9001 cgttgacgcc gacatcgccc cacaggccga cgagcaggag atttgtgtga acaagaagtt
     9061 gacctggagc ctggcggcga tcgccttcgc cactgccgcg gccgcccttg aaacggcgac
     9121 gccgcccaac cgcctcgcgc cgacccagga ccacgcccag accgcctacc tgagctccca
     9181 gctcctcgcc cgctaccatt acaaggcggt tcccctcgac gacgccctgt cggaaaagat
     9241 cttcgagcgc tacttcaagt ccctcgaccc ggagcgcctg ttcttcaccc aggccgacat
     9301 cgacgccttc gccgacgccc gcaccacgct ggacgacgcc atcctgcagg gcaacctggg
     9361 cgtccccttc gccatcttcc agcgcttcga gcagcgcgtc ctcgagcgcc tcacccaggc
     9421 gcgggaagtg ctcgccggca gcttcgactt caatgtgaag gagagctacg gctacagccg
     9481 cgacaaggcg ccctggccca aggacgacgc cgaggcccgc gacctctggc gcaagcgcgt
     9541 caagaacgac tggctgcgcc tgcgtctggc cggcaaggat gacaaggcca tccgcgagac
     9601 cctcgagaag cgctacgaca actcccttgc ccgtgccggt cgcaacaagg ccgatgacgt
     9661 cttccagatc ttcatgaacg cctacgccat gtcgatcgag ccgcacacca actacctggg
     9721 tcgcgccgcc tcggaagact tcgacatcgg catgcgcctg tccctgatgg gcatcggcgc
     9781 catgctccag gagcgggacg agtacatcac catccgtgag ctggtccccg gcggccccgc
     9841 cgcccgctcc ggccagctcg cccccggcga ccgcattgtc ggcgtcggcc agggcgaatc
     9901 cggcgcactc gtggacctgc tgggcacccg cgtggacgag gccgtcaagc tgatccgcgg
     9961 cgagaaggac accaaggtcc gtctcgacat cctgcccgcc gacgccggcc cggatggtcg
    10021 ccacaagcag gtcgtgctgg tgcgcgacaa gatcaagctc gaagaacagg ccgccaagaa
    10081 atccatcctc aaagtcggcg aaggtgccgg tgcgcgcaag gtcggcgtca tcaccctgcc
    10141 cgccttctat gaggacttcg agggccgccg ccgcggcgac aaggactacc gcagcgccag
    10201 ccgcgacgtg gcccgcctcc tcgacgaact gcgcaaggac aaggtggatg gcgtgctcgt
    10261 cgacctgcgc aacaacggcg gcggctccct gcgggaagcc gttgaactga ccggcctgtt
    10321 catcgacaag ggccccgtgg tgcagcagcg tcgcgccgac ggacgcatcg gcgtggaaag
    10381 cgatgagcgc gccggcgtgg cctgggacgg ccccatgggt gtgctcatca accgcggctc
    10441 ggcctcggcc tccgagatct tcgccgccgc cctgcaggac tacggccgcg ccgccatcct
    10501 cggtgagccc agcttcggca agggcacggt gcagaccctg gtcaacctcg acgaggtggt
    10561 ccgcaacgac aagccccgct tcggcgagct gaagatgacc gtcgcccagt tcttccgcgt
    10621 gaacggcggt accacccagt tgcgcggcgt cacgccggac atccgcctgc cgtccgtcag
    10681 cgacgacgag agctatggcg aatccagctt cgacaatgcc ctgccgtggg tcaagatcga
    10741 cgcagccccg ttcacgccgg ccggcaatgt ggtgcccctg gtgcccgatc tgcagcgccg
    10801 ccatcaggcc cgcgtggccc gcgacaagga ttttcaatac ctcctggaag acgtggccga
    10861 gttccgccgg cttcgggaca gcaaacagat ctccctcaac gaggccgagc ggcgcaaaga
    10921 gcgggacacc caggaggccc gcctcaaggc ccgcgaaaag accggcacaa aggacaagct
    10981 agccaatttg cgggacgacg gactgcaggc cggcgagcgc agcctcgccg cggacctcgc
    11041 ggccgagaag gcgcgcaagg agcgcaagga cgtcctgctc caggaagcag ccagcatcgt
    11101 cggggacgag gtcgacctgc aggcatctgc cacgccccgc ctcgcgctga aggaacgccg
    11161 ccccggacag ccggtgattc gctgagccag gcgcaggtcc gcaccggacc ccgtcgaccg
    11221 cgcggcgcgc ccaccgaccc gcggtcggcc ccccatctac aggtcagcgt gatcggtgtc
    11281 ccgccctgta gcaaatggca cccttgtggt cagggactcc gtgcgcgaga gtgaaatcac
    11341 tgcacactcg acaccggaga ccgacatgat cactggcaag ggcgcctttt gcgcgctgat
    11401 gttctcggca ggcatcctgt ctgcccctgc agcgacggcc gacgagggcc ctcgtctgag
    11461 cggctcgccc aaggtggaat tcaccgacag caccgccgag cgcatcggcc agaccaggcg
    11521 ggtggcgatc accaacgtaa tcctggaatt ccagaccaag gtcttcgccg aacacatcac
    11581 cgggcgcctg tccaagatgt acctcaagcg cggagactcc ttttcggaca acgaacttgc
    11641 gggctttgac ccacgacaac tcgaacaagc ggccaatcag gtttacaaaa agctcgtcac
    11701 cgatctcacc gcggccggat tcgaggtcgt gccggaagcg caggtcctcg cccagccggc
    11761 ctatcagaaa ttgcgcgaga ccatgggctg gccgcagggc taccactttg gcaacaagga
    11821 aggccacagc ctgatcgtcg gcccgacggc gctcaaaccc tatgttccgc ccctgtccga
    11881 acagggcacc ttcgccaaca ctcgccagga aaagggcgaa ctgcaggcac ccgccctcgg
    11941 gtggagcgac cgcgccagat ttgcaggaaa cagcagctac gcggccgggc tggaagtaca
    12001 gcttgccaag gcgctcaacg cccatgtcgt gaaggtctgg tacctcatcg gcttcggcaa
    12061 cgccaccgcc tccaccgact gggacgccat cagcacggcc cacttctcga caacggccac
    12121 ctcccgtacg ggcgccaccg cccagatgta cctgcgcgac gaggagacgc gcctggccgt
    12181 gcggctgccc gacggtgatg ccggctatgc ccgcgaatcg aagcgcatca acgccatcac
    12241 cggcggctac cgcccctacg acggcgacgt ggtcgtacgg ctcgacgaga cgattcccgg
    12301 cgaagccgac ttcctggcgg gcggcggcgt tcagaatgcc accacagagc gggacgccaa
    12361 ggcaggcatc ggaggactcc tgggcagcat ggccaagagc ctcaccggca ccatcgccga
    12421 caacagctat aaaaccaccg tcgacccgac ccgctttgcc gcggccgccg ccgacatggc
    12481 cggtcagctt cagcccttcc tgatcgcccg gatcaagcgc tgagaccttg cacctcatcc
    12541 cgcgggacgc aaagtcctgc ggccacatct gggcgcgcgg ccccgtccag gatacgtcgc
    12601 tgcaacgcac aaattcacac ttcatcggtc aagaatctct gcgccgagcc gtaagaaatc
    12661 caggttaccg agccccaggg tcaggcagcg tcccccctgt ccagcccggg cattccacaa
    12721 caacaaaaat ctggagtttc cccgtgctgc acctccggca acttgcgcgc gccacggctt
    12781 taggcgtcct gagcctcccc ctcccggccc tggccctcga agccggcggc ctcgacttca
    12841 ccggcaccgg cttcatgacc ctcgcggccg gccgcgtcct cagtggcacc cacgacgcct
    12901 cggtcgatca gggcttccgc tgcccctgct tcatctccga ctacgcccag cgcggcgtct
    12961 acgaatccgg gcagtggcgg gcgggaccgg attcgcgact gggcctgcag ggcacggtca
    13021 gtgccgccca ggggcgcttt gccctgaccg gccaggccgt cgcccgcggt gcggccaatg
    13081 gcgctgccga cctggaatgg ctctatgcca cggcggagct gagtggcaac ctcaccgccc
    13141 agatcggccg aaagcgcctg cccctgttca gctattccga agcccaggat gtgggcttcg
    13201 cgctgccctg ggtgcatctc ccaccgcagc tgtacggctg ggagatcgtc aactatgacg
    13261 gcgccagcct ggtctggcgc gagcagtggg gcgcctgggg cagcgcggtt caggtgttcg
    13321 gcggcaacga gacgcgccac gacagcggct actggcagat ctacaacggc aagttctcgc
    13381 gcaccgacgt gcgctggtcc gacatcctcg gcaccgaagt caccctcagc cgcgactggc
    13441 tgacgctgcg ggcggtgcgc atccagtcct acacccagaa caaggtggtg tccctgggcg
    13501 agacggcctt ttcagaccgc aagcgacagg tcatccaggg cctgtccatg ggcgtcgaca
    13561 gcggcaactg gtttgcccgg ggcgaattcc tgtacatcaa ccgcacgcag gattacggca
    13621 aggattacgc caacctcatc gccgtcggct accgtcatgg cccgtggcaa ccgctgctgt
    13681 cccgatcgga ctaccgccag cagctcgaca cggcgggcgt gccagagcgg catggcacct
    13741 ggtcggcggt gctgcggtac gacctgaccg aagagagcgc cctcaaggtc caggcagacg
    13801 tctggaccga caagaccgaa ccgggtttcg gcagcctcca cggcgacgcc cgactcctca
    13861 ccatcagcta cgacagggtg ttctgatgtc cctcccgttc tcctcctcgc gcgtccggca
    13921 gtccctttgc cacgccgcca cccttctggt cctgctcggc gcccccttca gcgtccggag
    13981 cgccgacgag atggttgtca tcatcaaccc ggagaatccg aacgtcatcg accgcgccta
    14041 cgtcgtcaag gtgtataccg gcgcggtcaa gggctggccg gacggatcgc cgatcttcct
    14101 gctcgattac ggccaggaca gtgagctgcg caagaccttc tacactgcgg tgatcggcaa
    14161 gagcccggcc gcgatgcgcg cgatctggtc tcagaacatc ttcaccggca aagccatgcc
    14221 gccccgcgtc acccaggaag acagcgagat gaagcacatc gtcgccagca accgcaacgc
    14281 cattggctac atccgcgcct cccaggtgga caagaccgtc aaggtgatcg aacggtaaca
    14341 cccgatgaat accgcaggcc ccgccaggac ccgggtctgg cagaacgaag cccggctcgt
    14401 cccggtcatc acctggatcg ccaccgtcac cgcgctgttg gtcgccattg ccgcaccgac
    14461 cggatacttc ctgcttgccc gcgaggccga actgcgcgat gcggccatgg ccgctcggct
    14521 gcacgccgct ttcgtcaccc agctgatcgc ggccagcccc gacgcatggc accaggaagt
    14581 cggcggcctg ctggacaccg acctcgcccc cagcgacgtc ccggagcagc gccgcatcgt
    14641 ggatgcagcc cagcagccga tcgccaccac cggcgcgccc ctcggcagac tggtcgtcga
    14701 gcgggcggcg gcgctgcggg gccccgacgg agcggtcggc gaggtgatca tcacccgctc
    14761 cctccgcccc acgctggtcc gcaccggtct tgtcgccctg ctgggcgtgg ccctgggctt
    14821 caccatctac ggctccctgc gcgttttgcc cctgcgggcc ctgcgtcgcg ccgtcgccgc
    14881 cctgcagcgg gaagagagca aggcgcgcga agaggccgag gaacagctgc gcgtcgtctt
    14941 tcagaacgcg gtggaaggga tcgccctgtt cacgccggac aggcgcatca cctcctgcaa
    15001 cccggccgtg gcgcgcatgt tcggccggcc cctcgagcac ctgccgggca tgttcatcac
    15061 cgatctgctg cgcccgttca gcggcgagga gcagggtacc gccttcacca cggggcaagg
    15121 ggagaccacg gcgttccgcg cctcgggcga agccttcccg gtggagatga gcgtcaatga
    15181 ggcccgtctg gcggaccgcc cccacttcat cgccatcctg cgcgacatca ccgaacgcaa
    15241 gcaggccgag cggcgcctgt cctacctggc caactacgac agcctgaccg gcctgcccaa
    15301 tcgcagcctg ttccgcgagc gcctggaacg ggccatgggc cgtacccggc gcaatggccg
    15361 tcagctggcc ctgatgttcc tcgacctgga ccgcttcaag aacatcaacg acaccctcgg
    15421 ccacgaggca ggcgatcagc tcctcaggca cgtggccaac gccctcatcg ccaacctgcg
    15481 cagcaccgac accgtcagcc gcgccacgct ggacgaatcc accgaaagta tcaccgtgtc
    15541 acgcctgggg ggcgacgaat tcacggtgct ggtggaggac atcaccagcc ccgccgccgc
    15601 cgcccaggtc gccacccgca tcctggccgc catcgcccag cccttcgaga tccatggcga
    15661 gcgcctgtac atctccacca gcatcggcgt caccctctat ccccacgaag ccgaagatct
    15721 cgacgggctc gtcaagcagg ctgacatggc catgtaccgg gccaaggaac tggggcgcaa
    15781 cacctatttc ttctacaacg acgaactcaa taccgagatg gccgagcgcc atgccctgga
    15841 ggccagcctg cggcacgccc tggagcggcg cgagttccac ctccattacc agcccaaggc
    15901 ctgcctgcgc agcggccgcg tcaccggcgt ggaggccttg ctgcggtgga caccgtcctc
    15961 gggacagcgc atcgggccgg accgcttcat tccgattctc gaggacatcg ggctgatcaa
    16021 ccaggtcggc acctgggtgc tgcgcaccgc ctgtgcccag atcgcagcct ggaagactgc
    16081 cgggctgccg cctctgcgcc tcgccgtgaa tgtgtcggcc cgccagttcc gtcagcccaa
    16141 cctgcccgaa cagatcgccg gcctgcttgc cgaacacggc ctcgcgccgg ccgacctgga
    16201 ggtggagctg accgagagca tgctgatgga ggacagcgag cagagccgcg acatcctctc
    16261 gcgcctgggt accatgggca tccagctggc catcgacgac ttcggcaccg gccactcgtc
    16321 cctcgcctat ctcaagcgct tcaacgttca cgccctcaag atcgaccgct ccttcgtcaa
    16381 ggacacgccg gaggatcccg aggacagtgc catcgccacc gccatcatcg gcctgtcggc
    16441 caacctgaag atccgcgtcg tcgcagaagg cgtggagacc caggcccagg ccgacttcct
    16501 gcagcagcag gggtgcgacg agatgcaggg ctatctgctc agcgcgcccc tccctccgcg
    16561 ggaatttgcc atctggctgc gccagcgcac ggcggccggg gccgtcgatc cggtcacctg
    16621 aacgtcgccc ccatgacgcg ggcgcaaatc cgcggcatca tggccccccc gcccccagga
    16681 gcgcgccatg gaaaacccgc aatcccttgt ccacttctgg ttcggaaccg atccggacga
    16741 tgccgtcacc gccgcccgcc aggccggcct gtggtggggc aaggacggcg ccgtcgatgc
    16801 ggagatggcc cgtcgctacg gtcacctgcc ggacgatgcc ctggcggggc gcctggacgg
    16861 gtgggcggcc acaccggccg gccgtctggc gctgatcctc gtcaccgacc agttcccgcg
    16921 caacatcttc cgcggcacgg cgcaggcatt cgcgttcgat gcgacggccc gccgcctgtg
    16981 ccgcgaagga ctcgcccacg gccaagaccg ggccctgcgc cccatcgagc gggtgttctt
    17041 ctacctgcct ctggaacact ctgaagacgc ggacgaccag gccctgtcgg tcaccctgtt
    17101 cgaagcgctc gcggccgagg tggccgtgcc gctccagccg atcttcgacg ggtacgccga
    17161 ctatgcccgc cgccatcggg acattgtggc ccgctggggc cgctttccgc atcgcaatgc
    17221 ggtgctgggg cgggcctcga ccgcggccga agacgccttt ctgcaggaac ccggctcgtc
    17281 gttctagaag gacgcgtccc agtccggcgc ggggagctcc tcgaaggcgt tgcgcaggca
    17341 ctcgccccaa cggttccgca ggtcgttgaa atacgtgttc tgcgcagtga tcgtcacctg
    17401 cttgccaacc ttcagcgcat cccgttcata gatcagcatg tcgagcggga gccccaccgt
    17461 caggttggac ttgatggtgg aatccatcga gatcagcgcg cacttggccg cctggtcgat
    17521 gggcgtttcg ctggtgatga cccggtccag gatcggcttg ccgtatttcg actcgccgat
    17581 ctggaagtaa ggcgtctcgc gggtcgcctc gatgaaattc cccaccgcgt agatctggaa
    17641 caggcgcggc tcctcgccgg cgatctggcc gccgagaatc agcgaggcgt tgaagtcgat
    17701 gccgaagtcc cgcagcgcct gcgcgtcgcg ggcgtggatc tcgcgcaggc agtcgcccac
    17761 gtggcgggca gcctcgaaca tgttcttgac cgtgtggagg ttcggccccc gcgactgctc
    17821 cagccgctcc cgcagcaggt tgaccaccga ctggctgatg gacaggttgc ccgcggacag
    17881 caggaccagc atccgctccc cgggcttttc gaagacctgc atcttgcgga aggtgttgat
    17941 ctggtccacc cccgcattgg tccgcgaatc cgacaggaag accagccctg cgtccagacg
    18001 catggccacg cagtaggtca ttgcagaccc tcgacgggcg ccaggaagtc gcggttgatc
    18061 cctgcgccga tttcgtagat gcgatccatg aagtcggaca ggaactcgtg caggccgcct
    18121 gccaggaagt cctccacaca cccgtaccgc agccgcgcgt agagcatgcc cgcacgccgc
    18181 tgggtctcgc cggaatggct gttggcgagg agatccaggt tcttgaccac gccgcgcagg
    18241 cagaagtgca gcgaacgggg catgtccggg tgcaggacca gcagctcggc cacccgttcc
    18301 ggcgtgatca cgtcgcgata gaccttgcga tagacctcga agccggacac cgaacgcagc
    18361 agggcacccc actggtagaa gtcggtgatg gcgtcctcgc tgtcgtggtt gacgggcagc
    18421 agtacgtgga acttcacgtc aaggatgcgc gccgtgttgt cggcccgctc cagcagggtg
    18481 ccgaggcgga tgaagttgta ggcctcatcc tgcagcatgg tgccggacgt ggtgccgcgg
    18541 ttgagggagg cccgcatctt gacccagtcg aagaactcgc ccggaccgtt ggccatgcgc
    18601 tgctcgtggg actggccacg cagttcgatc caggtggcgt tgagggtctc ccacatctcg
    18661 gacgtgatgg tgccgcgcac cgcgtgggca ttctcacggg ccgcccgcag gcagcagaag
    18721 atggacgccg gattggccgg gtcgctcacc atgaagtgca gcaccttgtc ggcggtggtc
    18781 tcgttgccat gtttggcgga gtaggccgcc tccagactgt tcagggcgat caccgcgcgc
    18841 cagttctcgg acgcggcggc cggcgcctgg ggcaccaggg aattctgcca ggtcacgtcg
    18901 agcaggcggg ccaggttctc tgcccgctca atgtagcggg ccatccagaa gaggtgatcg
    18961 gcggttcttg agagcatgtt cttattcctc cagcacccag gtgtccttgg taccgccacc
    19021 ctgggatgaa ttcacgacga gggaaccttc ggtcagcgcc acgcgggtca ggccgcccgg
    19081 caccatgtcc acatccttgc cggagagcac gaaggggcgc aggtccagat ggcgcggggc
    19141 cacaccgctt tcgacgaagg tcgggcagtt ggacagggcc agtgtgggct gcgcaatgta
    19201 acgcgccgga tgcgccgtca acaggccgcg gaagcgttcg atctccgccg tgctggcggc
    19261 cgggccgacc agcatgccgt agcccccggc gccatggact tccttcacca ccagctcggg
    19321 caggtgcgcc agggtatagg ccagatcctc cggcttgcgg cactggtagg tcggcacgtt
    19381 attgagcttg ggctcttcgc ccaggtagaa gcggaccatg tccggcacat aggggtagat
    19441 cgacttgtcg tctgccacgc cggtgccgat ggcgttggcg agggtcacgt tgccggcctt
    19501 gtaggccgcc agcaggccgc gcacacccag catggaatcg ggcctgaaca ccgtgggatc
    19561 gaggaagtcg tcgtccacgc gccggtagat cacatccacg cgacggcggc cctgggtggt
    19621 acgcatgtag acggtgtcgt cttccacgaa caggtcacgc ccctccacca gctcgacgcc
    19681 catctgctgg gccaggaagg tgtgttcgaa gtaggcgctg ttgtaggccc ccggcgtgag
    19741 cacgaccacg gtgggatcgg agatgccctt gggcgccact gcgcgcagct tctccagcag
    19801 caggtcggga tagtgctgca ccggtgccac cttgtggatg gagaacagct cggggaagag
    19861 gcgcatcatc atcttgcggt cttcgagcat gtaggacacg cccgacggca cccgcaggtt
    19921 gtcctccagc acatagaact cgcccttgcc ggcccgtacc acgtccacgc cggcgatgtg
    19981 ggcgtagatg ttgcccacca cgtccacccc catcatctcc gggcggaact gggcattgtt
    20041 gagcacctgc tcggccggga tcacgccggc tttgaggatc tcctgatcgt gatagatgtc
    20101 gtgcaggaag gcattcaggg tctggacccg ctgccgcagg ccggctgaca ggtcgcgcca
    20161 ctccggggcg gggatcaccc gcgggatgat gtcgaaggga atcagacgct ccttgccgcc
    20221 atcgccgtcg gacgcatcgc cgtacacggc gaaggtgatg ccgaccttgc ggaacgccag
    20281 atcggcctct gcgcgcttgc gctggatacg ctccgccggc gaggacgcca gccatgccgc
    20341 atagcgctcg tagtgggcac gtaccccgcc ggcgccatcg tgcatttcgt tatagaaatt
    20401 catgctcggg cctccctgta cctaagaatg gaaagtctgt cgcagaatcc atgccaaggt
    20461 actctttcaa taaaattc
//
LOCUS       DAOQPA010000058        20460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1280_length_20460_cov_17.786131, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000058 DAOQPA010000000
VERSION     DAOQPA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20460
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_20460_cov_17.786131"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="PKX01_09610"
     CDS             <1..1854
                     /locus_tag="PKX01_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ76424.1"
                     /translation="RAASRRKSADVPDTPEKPVAPAAGDAPASPQGDAPPTLAEATPR
                     KRAAGKAKSPRAPSRRAPRGSARPPEPDNLSDTPPELPAARLAAPQAELVLESPAVRS
                     DGAVMADEALPVVPREPAAADPESRPEPAADAPDEAASSDDPTAPITAEGAVPEAGDE
                     SGGPRRKRKRRRGRGGSDTDGDAAVSDGEAADAALMDSAAEALAPSFADAIPEPAEPP
                     PLTIVSLGLDLPPALSAAAREALAGARVILGTAEALDRLSGLSLSAPQRLCGAETAHL
                     DVRNAGHAGAVLVCVGDGAGDGPAAELLADLGPAHVRVLPGVGRVQAACAALGLSAEI
                     VTLVDLRRAPLAALRGQLRAHRLLAVPVADAERPAAVARLLLESGFPGARVWVCEFNG
                     TAPRATAWLASELVELREPLDPHAVLIVATGPSAGVFTELPGLSAAALGDDAGAALPL
                     AVRVLALAWLQPAAWETGWAISDGEASVALEWARAVPTARVRAVGVDPTLLGLSLARV
                     GVGENLAAIPSAMPIHAAAWPAPEAVFIRGSAGLPEWLAMAWARLAPGGRLVVSAEDD
                     QARADLVTFAAQVPAEAWQELSQSRGECIAGRLRFVPDAPVRLALWRKPAT"
     gene            1891..2682
                     /gene="cobA"
                     /locus_tag="PKX01_09615"
     CDS             1891..2682
                     /gene="cobA"
                     /locus_tag="PKX01_09615"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMZ76425.1"
                     /translation="MSDSTLPPSRGAARPGWVYLVGAGPGDAELLTLRAARLLLEADA
                     LVYDNLVSKEVLACVPAGTARYYVGKKASNHSLPQEGINALLVSLAREGRRVVRLKGG
                     DPFIFGRGGEEVEELVEAGIPFEVVPGITAASGIGAYAGIPLTHRDHAQSCVFVTGHL
                     KDGTVDLDWDMLARPKQTLVFYMGITRLTDICSQLIAHGLAPTTPAAVVRHGTSPDQR
                     VVTADLATLPEAVKRAGITPPALTLVGSVVSLQGRMAWFNPEGGA"
     gene            2679..3980
                     /locus_tag="PKX01_09620"
     CDS             2679..3980
                     /locus_tag="PKX01_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HMZ76426.1"
                     /translation="MNPRFPDASCPALFVAAPASGQGKTTVTAALARLHARQGRRVRV
                     FKCGPDFLDPQIHAVASGHPVHNLDLGMCGSADAAWRLHAAAQDADLILVEGVMGLFD
                     GTPSGADIARYFGIPVMAVIDARAMAQTFGALVHGLATWEPGLPFSGVLANHVGSPGH
                     ADLLRDSLGGSFAWYGALPQDADAALPERHLGLLQAAEIADLGARLDRLADHLARTGA
                     ADLPPPVSFAGMPPADVPPLLAGRTVAIARDAAFGFIYPANLDLLRALGAELRFFSPL
                     AGESLPPCDALWLPGGYPELHAATLAAHDAWHADLAAHKAAGKPILAECGGMMSLFAS
                     ITDREGVTHPGAGLLPGHTRMQDRLAALGTQIVLLPEGRLTGHTFHYSRSETPLEPLA
                     RAHTPAGREGEAIYRVGRLTASYVHFYFPSNPTAAARLFGA"
     gene            complement(4004..4861)
                     /locus_tag="PKX01_09625"
     CDS             complement(4004..4861)
                     /locus_tag="PKX01_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ76427.1"
                     /translation="METPHRSDLAGTTALVTGASSGIGRVTARELACRGATVVLACRS
                     EARTQPVLAEIARLTGRADAAVWLPLDLADFASVRACAEGFLRLERPLHLLVNNAGLA
                     GARGLTRDGFEMAFGVNHLGHFLLTRCLEDRLITSAPARVVTVASRAHRGAGGLDWAA
                     LRRPTRSLTGIREYAASKLANILFSAALARRLADRGVGAYAVHPGVVDTEVWRTVPGA
                     LRPLLRLRGLLTPEDGARTSLHCCVDALPGESGLYFARSSPVTPSRRARDGALADELW
                     QRSEAWVAS"
     gene            5015..5815
                     /locus_tag="PKX01_09630"
     CDS             5015..5815
                     /locus_tag="PKX01_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMZ76428.1"
                     /translation="MVPHASQAETRVAVLADCDNTQPPIVEYALRVAAQFGRVVLRRG
                     YGNHSTLARTWQEVLVRQAFTPCLQYTYAAGKNTSDIALALDALEILFDDRADKFCLV
                     TSDSDFAGLCRKLRERGATVCIVGEAKTPEALRNASDQFFEWAPAADTEAGNGTGPEQ
                     PAGAATKGEAARGGMIKRRPRFVVDAVALLAGNSSEGQVHLSALGQYLKRTDPGFSPA
                     NFGHSGLLDMLRTYDLLALHRADGGHYTVSLVPEADRVVARPAGSDTP"
     gene            5911..6384
                     /locus_tag="PKX01_09635"
     CDS             5911..6384
                     /locus_tag="PKX01_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ76429.1"
                     /translation="MNRPFKVLGVQQIAIGGPSKDRLRTLWVDMFGLEVTGNFKSERE
                     NVDEDITAMGAGPFKVEVDLMQPLDPEKKPAVHTTPLNHVGLWIDDLPKAVEWLSANG
                     VRFAPGGIRKGAAGFDITFLHPKGNEEFPIGGEGVLIELVQAPPEVVDAFARLAG"
     gene            6506..8965
                     /locus_tag="PKX01_09640"
     CDS             6506..8965
                     /locus_tag="PKX01_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ76430.1"
                     /translation="MRLVSCRPPPWLCAGCGRLLLLLVMLVGLVVPAVAQDEAHPGDT
                     VTRFRAALSAEERAWLDAHPVLRAGNGPDFQPFYAWQAGDRYAGPNADYLELLGRRTG
                     LQFRMVRFPEFPAVLHALDSGAIDLIPTLTPTESRRKRYRFTGGYMHSPAVLVTRSSG
                     GPTGLPADLRGVRIAIERGHASREVLLRTRPGARLVDVDDAASALQAVATGEADAYVG
                     LLAVAHYYMERLSLANLQVRQRFDADLSAMAMAVPRDQTLLAGILRKAMAFVSDAEGH
                     ALLRRYLPTGTGIPGEAFHLTAAEQAWLQAHGPIRVGYDQAFYPLSYTNLHRQAEGYS
                     IELFRLLRDKVGLSVDEVAGTWSAVLDKALRNELDVLVGVASTPERREKLLFVGPYLT
                     TPTAIVTHSDFQQVWDLGEFAGRKLALLESHFLRERIRSAYPTIQLVEVPTQEDALRR
                     VAAGSADAAIGNLYAINRLIQSHFLGKLYIAGHVPDGDSELYLGVQPAAPELAAILGR
                     ALEALSPADIATARNRWLDTRYMPELPVRRFALYLTVPLAALVILLAISAFMTRRLRR
                     ALEACQAREASLAEQFETLAGAHARMEAVTGAVARDLRRPVFSLDDQVDLLRDALVHA
                     PAKVPAALDRLAEASRRIRAALVGLEGLAGSAAPPPRRAPVNLAALATALWPDLMARH
                     GQGHAVRLDCAGGPQVTGDPVLLRGVVRQLLSNAVKFSRHTLDARVTLRTLPGGAHGF
                     EIADNGPGIPPAEVAALFQPYQRLSNAGRVEGAGIGLAVARQLARRMGGDIQLVAQAG
                     GGACFRVSLEVQDVVPAGRPV"
     gene            complement(8981..9847)
                     /locus_tag="PKX01_09645"
     CDS             complement(8981..9847)
                     /locus_tag="PKX01_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HMZ76431.1"
                     /translation="MRFDNSRESDNIEDRRGDGGGGGLPIGGRGIGLGTVVLALVAMY
                     FGVDPRVVLNLAEQAPAPSAPAQHRAGPPANDPETVFVRHVLAETEDTWQTLFRQNGR
                     EYVDPKLVLFSGATRTACGTGQTAMGPFYCPADQKVYIDLAFYQELKSRFHAPGEFAQ
                     AYVIAHEVGHHVQNLLGISAKVQAARERAGEREANALSVRLELQADCLAGVWAKNADA
                     ARHIIEAGEVDQALRAASAIGDDTLQKQAQGYAVPDSFTHGSSEQRTRWFKRGMEAGD
                     LAACNTFQAKTL"
     gene            10190..11668
                     /locus_tag="PKX01_09650"
     CDS             10190..11668
                     /locus_tag="PKX01_09650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ76432.1"
                     /translation="MDFSSLGLSEPIAQAVLEAGYTEPTSVQLAAIPAALAGKDLVVS
                     SRTGSGKTAAFMLPSLQRLTAEPTIKARGPRVLVLTPTRELAVQVTDATKRYGKNLRY
                     AKAVSVVGGMPYPVQNKLLSAPYEILVATPGRLMDQMNRGRIDFGRLEILILDEADRM
                     LDMGFVDDIEAICAALPKERQTLFFSATLDGQVARMMQRLLKDPERIEIATAQTRHDN
                     IEQRLLVADDIGHKHRLLDGILRDVAVDQAIVFTATKRDADALTLSLEAQGHSVAALH
                     GDMNQRMRTRTLDQLRRGHLRVLIATDVAARGIDVRRISHVINYDLPKVAADYVHRIG
                     RTGRGGDTGVAISLAERRDVRLVRAIERYTRQTMAVHTLPGLEPRASLPTEERRPAGP
                     RRDGRSFGNGPRFGDRRPREGGFGAPRFEGDRPARDSREGGHAHRAAGDARHDGPRRS
                     FGQPAPRSDARGGFGGARFGERPAGRADSRPRREGRTFDRDR"
     gene            complement(11761..12648)
                     /locus_tag="PKX01_09655"
     CDS             complement(11761..12648)
                     /locus_tag="PKX01_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76433.1"
                     /translation="MHDPSAPPSDDPDGLDALEAALSADPACIDAARKRVTAIRTTPV
                     PTAMVARDVHFPDVPAVTPPATTAPTTPPPKPGRGGGLLAELKQAVAEQKRVEADSSR
                     EEERVREVIGTALRRVFEYLHDLVGQLNQLRPPIPRSYTLLVTGTVFDGLSWQEGFTG
                     HRSQPHTNGDLLERVTLSYRLGGAGDTLIERTGSGIERLRQALFDFGFKTECTERRNA
                     RREVEFATFAVRSEISVQVQWRADFPRRQVIVEMRNLERLGTVEFALPAFALNPEFLD
                     EFGRMMLGQPNRFRDLLPR"
     gene            complement(12922..16230)
                     /locus_tag="PKX01_09660"
     CDS             complement(12922..16230)
                     /locus_tag="PKX01_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76434.1"
                     /translation="MSGTRTLSLRWRLIGPLIGVWAVGLALLVTVLYINLLDRFRGLV
                     EQRADTLVAAVYSAAETAREPADLRRFVSTLGAGRDVSSVTLLGGRPARVIAATDHGL
                     HDLGVDELPDRPLAAQISRVILERKGERVLDAERRSLVVLEPVLLMGTDGEHGRLEDG
                     VLALRLDATSLWRAARDTVLHLSVGLGAIFSAVALGAYLMLSRYVLRPVDGIRDALDR
                     RAAGEADVPVPIARPDELGELARTLGELLDTLRIRNRQLVLARDHHLGMLESFPHLAW
                     RAGLNGQCDWFNRAWLRFTGRALAQEVGSGWLENVHPDDRDRCARAFRRHIDAREPMA
                     LEFRLRHADGTYHWIASHGAPMFDDTGEFVGHVGSCYDLQASRDNAQALARMTRLYHA
                     LSATNKAIVHTRDADALLPQVCRIAVEYGELERAWIGFRSAPGQGWRIVAAHGRNGPL
                     RLDDPALRCRLPDAGGASPHIDNDMAQGCGACGETDGGACATFPIAGDPELDAMPDGA
                     LFLRTSERNFFDVPKVALLEEMATDIGFALRNLQRDRALRLAARVFENNSEGIVVIAP
                     DRRVVMINRAFTTLTGFTLDAVRNLPLALFDPAHHPADFEADLRDHAAAGEVWQGEAW
                     LRRADGEAMPCALTLSAVRSDAGGLTHYVAVFSDISQRKADEARIRFLASHDFLTGLP
                     SRAVLEPTVADAIAAATRSGRRAALCFLDLDRFKNVNDTLGHHTGDRLLVEVARRLGA
                     VLQDGECLLRHGGDEFVVVMPEAGDRNALAAAAARLIGALDAPFELAGCEFSLSASIG
                     VAVFPEDGHEPDTLLDRADAAMYRAKEAGRNAVEFFADGMAAPTRERLALELALRRAL
                     ERQELAVHYQPLVKAHDSSLTGAEALLRWRHPELGDISPARFIPLAEESGLILPIGTW
                     VLDTVCRDLAAWRAAGRTVVPVAVNLSALQFRRADFVATVSDILHRHAIPAELLILEV
                     TESMVMRDVERAAHQLGELKALGLSIAVDDFGTGYSSLAYLKRFPLDKLKVDQSFVRE
                     INAGPGDRAIVAAIVGLGHTLGLSLVAEGVETEEMAETLRGLGCDLLQGWLFGRAEPA
                     AVFSERLAGALRYA"
     gene            complement(16227..17219)
                     /locus_tag="PKX01_09665"
     CDS             complement(16227..17219)
                     /locus_tag="PKX01_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ76435.1"
                     /translation="MTPNRRRFLTLGAGTLAMAMTGGGCSSGSSGPIKFATNPWPGYD
                     LLYVARDKGFFQDAGLDVNLVDLTSLGASRRAFERRQVDGCGATLVEILLAADLSGVR
                     PQIAAVVDYSDGADVVIGQAAMSSPTSLKGARVAIEPGTLHIQLLSLALARAGLTIRD
                     VTLVSMPQNVMPAAFAEGRVQAAVCFPPESGQLLARGGKVLFHSGQVPGEVVDILVFD
                     GDFVARRGADVRAFVRAYYQAQDYAARHPDEAHAIMARRENTSLADLRDTLKGLRYVD
                     QADQARLLEPGGPVERSLIHLQGAMIELAQLRERHTLKDFLMARELLAARGALA"
     gene            17409..18413
                     /locus_tag="PKX01_09670"
     CDS             17409..18413
                     /locus_tag="PKX01_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HMZ76436.1"
                     /translation="MPAIELVCPAGSLPALKTAVDHGADCVYLGFKDDTNARNFSGLN
                     FDPKAMADGVRYAHDRGRKVLLALNTYPQTDTWQRWTGAVDRAAELGIDAVILADPGL
                     MAYARRVHPELRLHLSVQGSATSYEAINFYHAHFGIARAVLPRVLSLAQVEQVIAHTP
                     VEIEVFGFGGLCVMVEGRCALSAFATGESPNCNGACSPGKHVRWEQTPRGMETRLNGI
                     LIDRFADGERAGYPTLCKGRFEVNGETYYAIEEPTSLNTLELLPELARVGVRAIKIEG
                     RQRSPAYVAQVTRVWRAAIDRVLAGAGPFRVEPAWMAELNKVSEGQSHTLGAYYRPWK
                     "
     gene            18431..19336
                     /locus_tag="PKX01_09675"
     CDS             18431..19336
                     /locus_tag="PKX01_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HMZ76437.1"
                     /translation="MKLALGPLLYYWPRAATLSFYAEIAESPVDIVYLGETVCSRRHE
                     LRLADWLEVAETLAATGKEVVLSSQVLVESESDLKTLRRIVDNGRFRVEANDMGAVRL
                     LTAGDGARPDWIAGPTLNIFNPGTLGLFAGLGASRWVAPPELSRDQIAAVRAQLGLHM
                     ETEVFAHGRLPLAYSARCFTARHFNLQKDTCEFRCLQFADGIPLKTREGEPFLCLNGI
                     QTQSAHVHTLLGDLPAVVADGIDILRISPQGEHTAEVVTLFRDALEGLTPPQDALQEA
                     LPLLPEAPCNGFWHGRAGVEQFVTA"
     gene            19359..19835
                     /locus_tag="PKX01_09680"
     CDS             19359..19835
                     /locus_tag="PKX01_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMZ76438.1"
                     /translation="MKLPAFTLPSLVTRVGARLPQLPPTLALVAGLNAALDRLIPRDT
                     LEPLTGKRLLLRITDAGLALRFTLTPRGFRPVLDRRAPDLTISATTRDYLALALREED
                     PDTLFFSRRLLMEGETDLGLLVKNTLDAVDWEAVWRVVKSPIVGMTGASQTGSTVT"
     gene            complement(19811..20170)
                     /locus_tag="PKX01_09685"
     CDS             complement(19811..20170)
                     /locus_tag="PKX01_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76439.1"
                     /translation="MVYFFWADTPIAGAQCSHCHTILWTNPRTHPILSESVPAQVATH
                     HGEYRRYYEGKIARFLSSMPPCPHCGKRQFDRFINNTQFPRFPSGRDIPEGITSADLI
                     KVDPDSTLVYYVTVDPV"
BASE COUNT         2965 a         7325 c         7034 g         3136 t
ORIGIN      
        1 cgtgcggctt cgcgccgcaa gagtgcggac gttccggaca cccccgaaaa gcccgtcgcg
       61 cccgcggcag gcgacgcacc tgccagccct cagggcgacg ccccgccgac gctggccgag
      121 gcgacacccc gcaagcgtgc tgccggcaag gcgaaatccc cgcgcgcacc gtcccggcgt
      181 gcgccccgcg gcagcgcccg cccgcccgag cccgacaatc tctccgacac gcccccggaa
      241 ctgccggcgg cacgcctcgc cgccccccag gccgaactgg ttctggagtc gccagccgtc
      301 cgctccgatg gggccgtgat ggcggacgaa gccctgcccg tcgtgccgcg cgagcctgcc
      361 gccgcggacc cagaaagtag gcctgaaccg gctgctgacg ctcccgacga agcggcgtcg
      421 tcggacgatc ccaccgcgcc gatcacggct gaaggtgccg tgcccgaagc cggcgacgag
      481 tccggcgggc cgcgccgcaa gcgcaaacgc cgccgcggtc gcggcggtag cgacaccgat
      541 ggcgatgccg cggtctccga tggcgaggcg gccgacgctg cgctgatgga ctctgcggcc
      601 gaggcgctcg ccccgtcctt tgcggacgcc atccccgaac cggccgagcc cccgcccctt
      661 accatcgtga gcctggggct ggacctgccg ccggccctgt ccgccgcggc gcgggaggcg
      721 ctcgccgggg cccgcgtgat cctcggcact gccgaggccc tcgaccgcct gagcgggctc
      781 tccctgtcag caccccagcg cctgtgcggc gccgagacgg cgcatctgga cgtgcgtaac
      841 gcggggcacg cgggggccgt gctggtgtgc gttggcgacg gcgccggcga tggaccggcc
      901 gccgaactgc tggccgacct gggaccggcc cacgtgcgcg tgctgcccgg cgtcgggcgc
      961 gtacaggcgg cgtgcgcggc gctgggcctg tcggccgaga tcgtcaccct ggtggacctg
     1021 cgccgtgcac cgctggcggc cctgcgcggc cagctgcgcg cccaccgcct gctggcggtg
     1081 ccggtcgccg acgccgagcg ccccgccgcg gtggcccgcc tgctgctgga gagcggcttt
     1141 cccggtgccc gcgtgtgggt gtgcgagttc aacggcacgg caccgcgggc caccgcctgg
     1201 ctggcgtcgg agctggtcga actgcgcgag cccctggatc cccacgcggt gctgatcgtc
     1261 gcgaccggcc cgtcggccgg cgtattcacc gagctgcccg gcctctccgc cgcggcactg
     1321 ggggatgacg ccggggccgc gctgcccctg gcagtgcgcg tcctcgcgct tgcctggctg
     1381 cagccggccg cctgggagac gggctgggcg atcagtgatg gcgaggccag cgtcgccctg
     1441 gaatgggccc gcgccgtgcc gaccgcccgt gtccgcgccg tcggcgtcga tcccaccctg
     1501 ctcggtctgt ccctggcccg cgtcggggtc ggtgaaaacc tggccgcgat cccgtcggcg
     1561 atgccgatcc acgcggcggc ctggcctgca ccggaggccg tcttcatccg cggcagcgcc
     1621 ggcctgcccg agtggctcgc catggcctgg gcgcgccttg cgcctggcgg ccgcctggtg
     1681 gtgagtgccg aggacgatca ggcccgcgcc gatctggtga cgtttgcggc ccaggtgccc
     1741 gccgaagcct ggcaggagct gtcccagtcc cggggcgaat gcatcgccgg tcgtctgcgt
     1801 ttcgttcccg atgcgccggt gcgtctggcc ctgtggcgca agcccgccac ctgagtccgt
     1861 ccgcccgcac gattgatccg gagagcctcc atgtccgatt ccacgcttcc cccgtcccgt
     1921 ggcgccgccc ggcccggctg ggtctatctg gtgggcgccg gtcccggcga tgcagagctc
     1981 ctgaccctgc gcgccgcgcg cctgctgctg gaggccgacg ccctggtcta cgacaacctg
     2041 gtgagcaagg aagtcctggc ctgcgtgccg gccggcacgg cccgctacta cgtgggcaag
     2101 aaggcgtcca atcacagtct gccccaggaa ggcatcaacg cgctgctggt gagcctcgcg
     2161 cgggaagggc gccgggtcgt gcgcctcaag ggtggcgatc ccttcatctt cgggcgcggc
     2221 ggcgaggagg tggaggagct ggtcgaggcc ggcattccct tcgaggtggt gccgggcatc
     2281 accgcggcat cgggcatcgg cgcctatgcc ggcattccgc tgacccatcg ggaccacgcc
     2341 cagtcctgcg tcttcgtgac cggtcacctc aaggacggca ccgtcgatct ggactgggac
     2401 atgctggcgc ggccgaagca gaccctggtg ttctacatgg gcatcacgcg gctcaccgac
     2461 atctgcagcc agctcatcgc ccacggcctg gcaccgacca cgcccgctgc cgtggtgcgc
     2521 catggcacca gcccggacca gcgcgtggtc actgccgatc tggcgaccct gccggaggcc
     2581 gtgaagcgtg ccggcatcac gccgccggcc ctgaccctgg taggcagcgt cgtcagcctg
     2641 cagggccgga tggcctggtt caatccggag ggcggcgcat gaacccgcgc tttcccgacg
     2701 ccagctgccc ggcgctgttc gtcgctgcgc cggcctcggg ccagggcaag accaccgtca
     2761 ccgcggcgct ggcccggctg catgcgcgcc aggggcgccg ggtgcgggtc ttcaagtgtg
     2821 ggcccgattt cctcgatccg cagatccacg cggtcgccag cggccacccg gtgcataacc
     2881 tggacctggg catgtgcggc tcggccgacg cggcgtggcg tctccatgcg gcggcgcagg
     2941 acgcggatct gatcctcgtc gaaggcgtca tggggctctt cgacggcacg cccagcggcg
     3001 ccgacatcgc ccgctatttc ggcatcccgg tgatggccgt catcgacgcc cgcgccatgg
     3061 ctcaaacctt cggcgccctg gtgcatggcc tcgccacctg ggagcccggt ctgccgttct
     3121 ccggtgtcct ggccaaccac gtgggcagcc ccggccacgc cgacctgctg cgggacagcc
     3181 tgggcggcag cttcgcctgg tacggcgccc tgccccagga tgcggatgcg gccctgccgg
     3241 agcgtcatct gggcctcctc caggcggccg agattgccga cctgggcgcg cgtctcgacc
     3301 ggctggcaga ccatctggcc cgtaccggcg cggccgatct gccgccgccc gtcagcttcg
     3361 ccggtatgcc cccggccgac gtgccgccgc tcctcgccgg gcgcacggtc gccatcgccc
     3421 gggatgccgc cttcggcttc atctacccgg ccaatctgga cctgctgcgc gccctgggcg
     3481 ccgagctgcg cttcttctcg ccccttgccg gcgagagcct gccgccctgc gacgccctgt
     3541 ggctgcccgg cggctatcct gagctccacg ccgccaccct ggcggcccat gacgcctggc
     3601 acgccgatct ggccgcccac aaggccgccg gcaagccgat cctggcggag tgcggcggca
     3661 tgatgagcct gtttgcgtcc attaccgacc gggagggcgt cacccatccc ggcgccggcc
     3721 tgctgcccgg tcatacccgc atgcaggacc gcctggccgc cctcggcacc cagatcgtcc
     3781 tcctgccgga aggtcgcctc accgggcaca ccttccacta ttcccgcagc gagacgccgc
     3841 tggagccgct cgcccgcgcc cacacgccgg ccgggcggga gggcgaggcg atctaccgcg
     3901 tcggccgcct gacggcgtcc tatgtacact tctactttcc gtccaacccc actgcggcgg
     3961 cgcgtctgtt cggcgcctga gcatcgcgcc ggagggacag ccttcaactg gcgacccacg
     4021 cctcactgcg ctgccacagt tcgtccgcca acgccccgtc gcgggcgcgt cgggacggcg
     4081 tcaccgggct ggaccgggcg aagtacagac cgctctcgcc cggtagggcg tcgacgcagc
     4141 agtggaggct ggtgcgtgca ccgtcctcgg gggtgagcag gccgcgcagc ctgagcaagg
     4201 gacgcaaggc gcccggcacg gtgcgccata cctcggtatc caccacgccc ggatgcacgg
     4261 cgtaggcgcc gacgccacga tcggccaggc ggcgcgccag tgcagcgctg aacaggatgt
     4321 tggcgagctt ggacgcagcg tattcccgga tgcccgtgag gctgcgggtc ggccggcgca
     4381 gggccgccca gtccagaccg ccggcgcccc ggtgggcgcg gctggcgacg gtcaccacgc
     4441 gagccggcgc cgaggtgatc aggcggtcct ccaggcaacg ggtcagcagg aaatggccca
     4501 ggtgattgac gccgaacgcc atctcgaagc cgtcacgggt cagaccccgc gcgccggcca
     4561 gcccggcgtt gttcaccagc agatgaaggg gacgttcgag gcgcaggaag ccttcggcgc
     4621 aggcgcgcac cgaagcgaag tccgccaggt cgaggggcag ccagacggct gcatcggccc
     4681 ggccggtcag gcgcgcgatc tccgccagca cgggctgggt gcgcgcctcg ctgcggcagg
     4741 ccagaaccac ggtggcgccc cggcaggcga gttcgcgggc ggtgacacgg ccgatgccgc
     4801 tgctggcgcc ggtgacgagg gccgtcgtgc cggcaaggtc ggacctgtgt ggagtctcca
     4861 tcgggccagt atcgcggcgc cccatgaccg gcatgtgatc gtctccgggc ggccctgcgt
     4921 ctgccctgac cgattgcggg gatgacgcag gcgtggccgg cgtgcgacag tggcagcccc
     4981 ggcggcccgc ccgcctctcc ggaagaacca taccgtggtc ccgcatgcct ctcaggccga
     5041 aacccgcgtc gccgtgctgg ccgactgcga caacacccag ccccccatcg tcgagtacgc
     5101 cctgcgggtc gctgcccagt ttggccgcgt cgtgctgcgg cggggctacg gcaaccacag
     5161 caccctcgcc cggacctggc aggaggttct ggtccgtcag gccttcacgc cctgcctgca
     5221 atacacctat gcggccggca agaacacgtc ggacatcgcc ctcgccctgg atgcgctgga
     5281 gatcctcttt gacgaccgcg ccgacaaatt ctgcctggtg accagcgact cggattttgc
     5341 ggggctgtgc cgcaagctgc gggagcgggg cgccaccgtg tgcatcgtcg gtgaggccaa
     5401 gacgccggag gccctgcgca atgccagcga ccagttcttc gaatgggctc cggcggccga
     5461 cacggaggcg gggaacggca cgggtcccga gcagcccgcg ggtgcagcga ccaaggggga
     5521 ggcggcccgc ggcgggatga tcaagcggcg tccgcgcttc gttgtggacg cggttgccct
     5581 gctggctgga aattcgtccg aaggtcaggt tcacctgagc gcgctcgggc agtacctcaa
     5641 gcgcaccgac cccggcttct cgccggcaaa tttcgggcat tccggcctgc tggacatgct
     5701 ccggacttac gatctgctgg cgctccaccg ggccgacggc ggccattaca cggtgagtct
     5761 ggtgccggaa gccgaccggg tcgtggcacg tccggcgggg agcgatacgc cctgagcgcg
     5821 tgccgcaggc gcgctcccct tccgtcccga acccgcccgc ccgtagaatc ccccctccaa
     5881 acaccgatac aacgatagag ggagaaaacc atgaaccgac ctttcaaggt cctcggcgtc
     5941 cagcagatcg ccatcggcgg cccgtccaag gaccggctgc gcaccctgtg ggtggacatg
     6001 ttcggcctgg aagtcaccgg caacttcaag tccgagcggg agaacgtgga cgaggacatc
     6061 accgccatgg gcgccggccc cttcaaggtg gaggtggatc tgatgcagcc cctcgatccc
     6121 gagaagaagc ccgccgtgca caccacgccc ctgaaccacg tggggctgtg gatcgacgac
     6181 ctgcccaagg ccgtggaatg gctcagcgcc aacggtgtgc gctttgcccc cggtggcatc
     6241 cgcaagggcg ctgcgggctt tgacatcacc ttcctgcatc ccaagggcaa tgaggagttc
     6301 cccatcggcg gcgaaggggt gctgatcgaa ctggtgcagg cgccgcccga agtcgtcgac
     6361 gccttcgcgc gcctcgccgg ctgatccacg cgccccgtcc ggggcacccg gagcccgaga
     6421 cagatcgttc cattccgcgt ggaacagggc tccggaaagc gcatatcctg cgaacgttcg
     6481 gaacctcctc cggaaagact tcgtcatgcg cctcgtgtcg tgccgtccgc ctccctggct
     6541 gtgcgccggt tgcgggcggc tgctcctgct gctggtcatg ctggtcggcc tcgttgtgcc
     6601 ggctgtggcg caagacgagg cccatcccgg cgacacggtc acgcgtttcc gcgcagccct
     6661 gagcgccgag gagcgggcct ggctcgacgc gcatcccgtc ctgcgggcag gcaacggacc
     6721 ggattttcaa cccttctatg cctggcaggc gggtgaccgc tacgccggcc ccaacgcgga
     6781 ttacctcgaa ctcctcggcc gtcgtaccgg cctgcagttc cggatggtgc gcttccccga
     6841 attcccggcg gtgctgcacg ccctcgattc gggcgccatc gacctcatcc cgaccctcac
     6901 cccgaccgaa tcgcgccgca agcgctaccg cttcaccggg ggctacatgc attcgccggc
     6961 ggtgctggtg acccgcagca gcggcggccc caccggcctg ccggccgatc tgcggggcgt
     7021 ccgcatcgcc atcgaacggg gccatgcgtc ccgcgaggtc cttcttcgca cccggcccgg
     7081 cgcccggctg gtggacgtgg acgacgcggc gtcggcgctg caggcggttg ccaccggcga
     7141 agcggatgcc tatgtcggcc tgctggctgt cgcccactac tacatggagc gcctgtccct
     7201 ggcgaacctg caggtgcgtc agcgtttcga cgcggatctg tccgccatgg cgatggcggt
     7261 gccgcgcgac cagaccctgc tggcgggcat cctgcgcaaa gccatggcct tcgtcagcga
     7321 cgccgagggc cacgcgctgc tccgccgcta cctgccgacc ggtaccggca tccctggaga
     7381 ggcctttcac ctgacggccg ccgagcaggc ctggctccag gcccatggtc cgatccgcgt
     7441 cggctacgac caggccttct atcccctcag ctatacgaac ctgcaccgcc aggcggaggg
     7501 ctattccatc gagctgttcc gactgctgcg cgacaaggtc ggcttgtcgg tggatgaagt
     7561 ggcgggcacc tggtccgcgg tgctggacaa ggccctgcgc aacgagctgg acgttctggt
     7621 gggtgtcgcc agcacgccgg agcgtcgcga aaagctgctc ttcgtcgggc cctacctgac
     7681 caccccgacg gccatcgtga cccacagcga cttccagcag gtgtgggatc tgggcgaatt
     7741 cgccggacgc aagctggccc tgctggaaag ccacttcctg cgcgaacgca tccgcagcgc
     7801 ctacccgacg atccagctgg tggaggtgcc cacccaggaa gatgccctgc gccgggtggc
     7861 cgcagggtcc gcggatgcgg ccatcggcaa tctctacgcg atcaaccgcc tgatccagtc
     7921 ccacttcctc ggcaagctct acatcgccgg ccacgtgccg gacggcgata gcgagctgta
     7981 cctgggcgtc cagccggccg cgccggaact ggccgcgatc ctcggccgtg ccctcgaggc
     8041 cctgtcgccc gccgacatcg ccaccgcccg caaccgctgg ctcgacacgc gctacatgcc
     8101 ggagctgccg gtccgcaggt ttgcgctcta cctcaccgtg ccgctggcgg ccctcgtcat
     8161 cctgctggcc atcagcgcgt tcatgacccg gcgcctgcgg cgcgccctgg aggcctgcca
     8221 ggcccgtgag gcgagcctcg ccgaacagtt cgagaccctc gcaggggccc acgcccgcat
     8281 ggaggccgtg accggggccg tggcgcggga cctgcgccgt cccgtgttca gcctggacga
     8341 tcaggtggac ctgctccgcg acgccctcgt ccacgccccg gccaaggtgc ctgctgcgct
     8401 ggaccgcctc gccgaagcca gccggcggat ccgcgctgcg ctcgtggggc tggaggggct
     8461 ggccggcagc gcggcccctc cgccgcgacg ggcgccggtg aacctcgctg ccctggccac
     8521 cgcactgtgg cccgacctga tggcgcgcca cggccagggc catgcggtcc gtctggactg
     8581 tgccggcgga ccgcaggtga cgggcgaccc ggtgctgctg cgcggggtgg tgcgccagct
     8641 cctgtccaac gccgtcaagt tctcccgcca cacgctggac gcccgcgtca ccctgcggac
     8701 cctccccggt ggcgcgcatg ggttcgagat cgcggacaac ggtccgggca ttccgcccgc
     8761 ggaagtggcc gcgctgttcc agccctacca gcgcctgagc aacgccgggc gcgtggaagg
     8821 cgccgggatc ggtctggcgg ttgctcggca gctcgcccgg cgtatgggcg gcgacatcca
     8881 gcttgtcgcc caggcgggcg gcggtgcgtg tttccgggtc agcctcgagg tgcaggacgt
     8941 ggtcccggcg ggccggccgg tgtgagcgcc ggcgccgcgc tcagagcgtc ttggcctgaa
     9001 aggtgttgca cgccgccagg tcgccggcct ccatgccgcg cttgaaccag cgggtgcgtt
     9061 gctccgagct gccgtgggtg aaactgtcgg gcacggcgta gccctgggcc tgcttctgca
     9121 gcgtgtcgtc gccgatggcg gaggcggcgc gcagggcctg atccacctcg ccggcctcga
     9181 tgatgtgacg ggccgcgtcg gcgttcttgg cccacacgcc ggccaggcag tcggcctgca
     9241 gctccaggcg caccgacagg gcgttggctt cccgctcgcc ggcgcgctcc cgcgccgcct
     9301 gcaccttggc tgagatgccc agcaggttct gcacgtggtg gcccacctcg tgggcgatca
     9361 cgtaggcctg ggcgaactcg cccggcgcgt ggaagcggga tttcagttcc tggtagaagg
     9421 ccaggtcgat gtagaccttc tggtctgccg gacagtagaa cgggcccatc gcggtctgac
     9481 ccgtgccgca ggcggtgcgg gtggcgccgc tgaacagcac cagcttcgga tccacgtatt
     9541 cccggccgtt ttggcggaag agggtctgcc aggtgtcctc ggtctcggcc agcacgtggc
     9601 gcacgaacac ggtttccggg tcgttggccg gggggccggc gcggtgctgc gcgggcgcgc
     9661 tgggggccgg cgcctgttcg gccaggttca ggaccacgcg gggatccacg ccgaaataca
     9721 tggccaccag ggccagcacc acggtgccca ggccgatgcc acgtccgccg atgggcaggc
     9781 cacctccgcc gccgtcgccg cggcggtctt cgatgttgtc gctctcgcgg ctgttgtcga
     9841 aacgcatgca ggaatcctcg tgtcgaaagg gggctgcctg gaacggctgc tgcgatgcaa
     9901 caagttcctt gtggttccgc cgcattatcg ctatagttcg cgtcccggtg caaagcggtt
     9961 gcaccgagtc acatcgtccc tgaccccccg caaggctccc gattcgccgt gagcttgtcc
    10021 tgaccggtta gtgccgatat gcccgtcagg cagccccgga gtcccttccc ccgcccgttc
    10081 gcgcggaaag tcccactccc cgcaagcccc tgcgctccga gcgccgaggc ccgttgccgt
    10141 tttttcccgc ggccgccgca tccgcgcctc ctgcaaggaa agctactcca tggatttctc
    10201 cagtctcggt ctgtccgagc ccatcgccca ggccgtgctc gaagccggct acaccgaacc
    10261 cacctccgtc cagctggcgg ccatcccggc cgccctggcc ggcaaggatc tggtcgtgtc
    10321 cagccgcacc ggctccggca agaccgccgc cttcatgctg ccctccctgc agcgcctgac
    10381 cgccgagccc acgatcaagg cccgcggccc ccgcgtgctg gtgctcaccc ccacccgcga
    10441 gctggctgtg caggtcaccg atgccaccaa gcgttacggc aagaacctgc gctacgccaa
    10501 ggccgtcagt gtcgtcggcg gcatgcccta cccggtgcag aacaagctcc tctccgcgcc
    10561 ttatgagatc ctggtggcca cccccggccg cctgatggac cagatgaacc gtggccgcat
    10621 cgattttggt cgtctcgaga tcctgatcct cgacgaggcc gaccgcatgc tcgacatggg
    10681 cttcgtggac gacatcgagg cgatctgcgc cgccctgccc aaggaacgcc agaccctgtt
    10741 cttctccgcc accctcgacg gccaggtggc gcgcatgatg cagcgcctcc tcaaggatcc
    10801 cgagcgcatc gagatcgcca ccgcccagac ccgccacgac aacatcgagc agcgcctgct
    10861 ggtcgccgac gacatcggcc acaagcatcg tctgctcgac ggcatcctgc gcgacgtggc
    10921 cgtggatcag gccatcgtgt tcaccgccac caagcgcgac gccgacgccc tgaccctgag
    10981 cctggaagcc cagggccatt ccgttgctgc cctgcacggc gacatgaacc agcgcatgcg
    11041 cacccgcacc ctcgaccagc tgcgtcgcgg tcatctgcgc gtgctcatcg ccaccgacgt
    11101 ggccgcccgc ggcatcgacg tgcgccgcat cagccacgtc atcaactacg acctgcccaa
    11161 ggtggcggcc gactacgtgc accgcatcgg ccgcaccggc cggggcggcg acaccggcgt
    11221 cgccattagc ctggcggagc ggcgtgacgt gcgcctggtg cgcgccatcg agcgctacac
    11281 gcgccagacc atggccgtgc acaccctgcc cggtctggag ccccgcgcca gcctgcccac
    11341 cgaagaacgt cgcccggccg gcccgcgccg tgacgggcgc agcttcggca acggcccgcg
    11401 cttcggcgac cggcgtccgc gcgaaggcgg tttcggcgcc ccccgtttcg aaggggatcg
    11461 ccccgcccgg gacagccgcg aaggcggcca cgcgcaccgc gcggcgggcg atgcccgtca
    11521 cgacggcccg cgtcgcagct tcggccagcc ggccccgcgt tccgatgccc gtggcggctt
    11581 cggtggcgcc cgctttggcg agcgtcctgc cggccgcgcg gacagccgtc cgcgtcgcga
    11641 gggtcgtacc ttcgatcggg accgctgaat cccggtggga cgcgcgcctg cgcgcgccgt
    11701 cccgaccgcc agttgaaagg gcgccctcgg gcgccctttt tttgatgctg aacgccgtgc
    11761 tcagcgcggc agcagatcgc ggaagcggtt gggctggccc agcatcatgc ggccgaactc
    11821 gtcgaggaac tcgggattga gggcgaaggc gggcagggcg aactccaccg tgcccaggcg
    11881 ttccaggttg cgcatctcga cgatgacctg acgccgcggg aaatcggccc gccattgcac
    11941 ctgcacggaa atctcgctgc gcaccgcgaa ggtggcgaac tccacttccc ggcgggcgtt
    12001 gcggcgttcg gtgcattcgg tcttgaagcc gaagtcgaac agggcctgac gcaaacgctc
    12061 gatgccactg cccgtgcgtt cgatcagggt gtcaccggct ccgccaaggc ggtaggagag
    12121 ggtcacgcgc tccagcaggt cgccattggt gtggggctgg ctgcgatggc cggtgaagcc
    12181 ttcctgccag gacaggccgt cgaacacggt gcccgtgacc agcagcgtat agctgcgggg
    12241 aatgggggga cggagctggt tgagctgacc gaccagatcg tgcagatatt caaagacgcg
    12301 gcgcagggcc gtgccgatga cctcccgaac ccgttcttcc tcccgcgagg agtctgcctc
    12361 gacgcgcttc tgttcggcga cggcctgctt cagctcggca agcagccctc cgccgcggcc
    12421 aggcttcgga ggcggtgtcg tcggcgcggt cgtggcgggc ggcgtcacgg ccggaacgtc
    12481 cggaaagtgc acgtcccgcg ccaccatggc ggtcgggacg ggtgttgtgc ggattgccgt
    12541 gacacgcttg cgcgcggcat cgatgcaggc gggatcggca gacagggccg cttccagggc
    12601 atcgagcccg tcggggtcgt cggaaggggg cgcggaaggg tcgtgcatcc ggccggattc
    12661 gggaagggga ttcatgtttc agggaagttc gcaggtccgg ttcgggccgg acagggaatg
    12721 tcactgctcg tttcagggcg atgatcgccc aagtcgtacg gttctgtcat ctcctgcgcg
    12781 ctattggggg ccatgagcgc acgacaggga cgcctgtcca gaagcaccgc gggcggaggc
    12841 acgcgggcct ggcgtgcggg cgcggcgcca gcggcgggtg tcggaggggt ggagccggcg
    12901 gactgcgctc cgaacgggtg ctcaggcata ccgcagggca ccggcgaggc gctccgaaaa
    12961 gacggctgcc ggctcggcgc gcccgaacag ccaaccctgg agcaggtcgc atcccaggcc
    13021 gcggagggtt tccgccatct cttcggtctc gacaccttcg gccaccagac tgagtcccag
    13081 ggtgtgaccg agcccgacga tggccgcgac gatggcccgg tcgcccggcc cggcgttgat
    13141 ctcccgcacg aaggactgat ccaccttgag cttgtcgagg ggaaaacgct tgagataggc
    13201 gaggctggaa tagccggtgc caaagtcatc cacggcgatg gacagcccca gggccttgag
    13261 ttcgcccagc tggtgtgccg cccgctccac gtcgcgcatc accatgctct cggtgacctc
    13321 caggatgagc agctcggccg gaatggcgtg gcgatgcagg atgtcggaga ccgtggcgac
    13381 gaaatccgcg cgccggaact gcagggcgga gaggttcacc gccaccggga cgacggtccg
    13441 tcccgcagcc cgccatgcgg ccaggtcgcg gcacacggtg tcgagcaccc aggtgccgat
    13501 ggggaggatc aggccgcttt cctcagccag gggaatgaac cgcgccggcg agatgtcgcc
    13561 cagttccgga tggcgccagc gcaagagcgc ttcggcgccc gtcagggagc tgtcatgggc
    13621 cttcaccagc ggctggtagt ggaccgccag ttcctggcgt tccagggcgc ggcgcagggc
    13681 gagctccagg gccaggcgtt cacgggtggg cgcggccatg ccgtcggcaa agaactccac
    13741 ggcgttgcgg ccggcttcct tggcccggta catggcggcg tcggcccggt cgaggagggt
    13801 gtcgggctcg tggccgtctt cggggaagac cgccacgccg atgctggccg acaggctgaa
    13861 ttcgcagccg gccagttcga agggcgcatc gagggcgccg atgaggcgtg cggccgccgc
    13921 ggcaagggca ttccggtcgc cggcctcggg catgaccacg acgaattcat caccgccgtg
    13981 gcgcaacagg cattcgccgt cctgcagcac ggcgcccagc ctgcgggcca cttccaccag
    14041 caggcggtcg ccggtgtggt gcccgagggt gtcgttgacg ttcttgaagc gatccagatc
    14101 gaggaagcac agggcggcgc gccgtccgct gcgggtggcg gccgcgatgg cgtcggcgac
    14161 ggtcggttcg agcactgccc gggagggcag gccggtgagg aaatcgtgac tggccaggaa
    14221 gcggatgcgt gcctcgtcgg ccttgcgctg gctgatgtcg gagaacaccg ccacgtaatg
    14281 ggtcagtccg cccgcgtcgg agcggaccgc gctgagggtc agggcgcagg gcatggcctc
    14341 gccgtcggca cggcggagcc acgcttcacc ctgccagact tcgccggcgg ccgcgtggtc
    14401 gcgcaggtct gcctcgaagt cggccgggtg atgggccgga tcgaagagag ccagaggcag
    14461 gttgcgcacg gcatccaggg tgaagccggt gagggtcgtg aaggcccggt tgatcatcac
    14521 cacgcggcgg tccggggcga tgaccacgat gccttcggag ttgttctcga atacccgcgc
    14581 agccaggcgc agggcgcggt cgcgttgcag gttgcgcagg gcgaagccga tgtcggtggc
    14641 catctcctcc agcagggcga ccttggggac gtcgaagaaa ttgcgttccg atgtacgcag
    14701 gaagagggcc ccgtccggca tcgcgtcgag ctcggggtcg ccggcgatgg ggaaggtcgc
    14761 acaggcaccg ccgtccgttt cgccgcaggc gccgcagccc tgcgccatgt cgttgtcgat
    14821 gtgggggctg gccccaccgg catcgggcag gcggcagcgc agcgcggggt cgtcgaggcg
    14881 aagcgggccg ttgcggccgt gggcggcgac aatccgccag ccctggcccg gtgcgctgcg
    14941 gaagccgatc caggcccgct cgagttcgcc gtactccacg gcgatgcggc acacctgggg
    15001 cagcagcgca tcggcgtcgc gggtatgcac gatggccttg ttggtggccg acagggcgtg
    15061 atacagacgg gtcatgcgcg ccagggcctg ggcattgtcg cgtgaggcct ggaggtcgta
    15121 gcaggagccc acgtggccga cgaactcgcc ggtgtcgtcg aacatgggtg cgccatggct
    15181 ggcgatccag tgataggtgc cgtcggcgtg gcgcaggcgg aactccaggg ccatgggttc
    15241 gcgggcgtcg atatggcgac ggaacgcgcg ggcgcagcgg tcccggtcat ccggatgaac
    15301 gttttccagc cagccgctgc ccacctcctg ggccagcgcg cggcccgtga agcgcagcca
    15361 ggcccggttg aaccagtcgc attgaccgtt gaggccggcg cgccaggcga ggtgagggaa
    15421 gctctccagc atgcccaggt ggtgatcgcg ggcgaggacc agctggcgat tgcggatgcg
    15481 cagggtgtcg agcagttcgc cgagggtccg ggccagttcg ccgagttcgt cggggcgggc
    15541 aatcggaacc gggacgtcgg cctcgccggc ggcacggcgg tcaagggcat cgcggatgcc
    15601 atccaccgga cgcagtacgt aacgggacag catcaggtag gcgcccaggg cgacggcact
    15661 gaagatggcc cccaggccga cggacaggtg cagcacggta tcgcgggcgg cgcgccacag
    15721 gctggtggca tcgaggcgca gggccagcac gccgtcttcg aggcggccgt gctcgccgtc
    15781 ggtgcccatc agcaggaccg gttcgaggac caccaggctg cgccgctcgg cgtcgagcac
    15841 ccgttcgccc ttgcgctcca ggatcacccg ggagatttgt gccgcgaggg gtcggtcggg
    15901 cagttcgtcc acgcccaggt cgtggagccc gtggtcggtg gcagcgatga cccgtgccgg
    15961 gcgtccgccc aggagggtga cgctgctcac gtcgcgcccg gcgcccaggg tggacacgaa
    16021 gcggcgcagg tcggccggtt cgcgggcggt ttcggcggcg gaatacacgg cggcgacgag
    16081 ggtgtcggcg cgctgctcca cgaggccgcg gaagcggtcg aggaggttga tgtagagcac
    16141 cgtcaccagc agcgcgagac ccaccgccca gacgccgatc agcggtccga tcaggcgcca
    16201 gcgcagggac agggtgcgcg tgccgctcat gccagggcac cccgtgcggc gagcagctca
    16261 cgggccatga ggaagtcctt gagcgtgtgg cgttcgcgca gttgggcgag ctcgatcatt
    16321 gcgccctgca gatggatcag cgagcgctcg accggtccgc cgggttcgag cagacgcgcc
    16381 tgatccgcct ggtccacgta gcgcaggccc ttgagggtgt cgcgcagatc ggcgagggag
    16441 gtgttctcgc gtcgcgccat gatggcgtgg gcctcgtccg gatgcctggc cgcgtagtcc
    16501 tgtgcctggt aataggcacg cacgaaggcg cgtacgtcgg cgccgcgacg ggccacgaaa
    16561 tcgccgtcga agaccaggat gtccaccacc tcaccgggca cctgcccact atggaagagc
    16621 accttgccgc ccctcgccag cagttgcccg gattccggcg gaaagcacac cgccgcctgc
    16681 acgcgccctt ctgcaaaggc ggcgggcatg acgttctggg gcatggatac cagggtcacg
    16741 tcacggatcg tgaggcctgc acgggcgagg gcgagggata gcagctggat gtgcagggtg
    16801 ccgggttcga tcgcaacccg cgcgcccttg aggctggtgg gggacgacat ggcggcctga
    16861 ccgatcacca cgtcggcgcc atcggaatag tccacgaccg ccgcaatctg cgggcggacg
    16921 cccgacaggt cggccgccag caggatctcg accagggtcg cgccgcagcc gtccacctgg
    16981 cgtcgctcga aggcccgccg tgaggcgccg agggaggtga gatccacgag gttcacatcc
    17041 aggccggcgt cctggaagaa gcccttgtcg cgggcgacat agagcaggtc gtaaccgggc
    17101 caggggttgg tggcgaactt gatggggccg ctgctgcccg aactgcatcc tccgccggtc
    17161 atggccatcg ccagcgttcc ggcacccagg gtcagaaagc ggcgacgatt gggagtcatg
    17221 gcagggacgt cattcaaacg tatcagtgaa tgctgaagta tgcactgcca cccctacgtg
    17281 gcgcatccga ctaaaggcgt aaattcccct atattcctgc ggcccgcgcg ctggccgttg
    17341 acccttgtca aggctgctgc agatggcccg gcctatagtc cgcccgcgcc atttctgccg
    17401 ccgatcccat gcccgccatc gaactggtct gccctgccgg cagcctgccc gccttgaaga
    17461 ctgccgtgga ccacggcgcc gactgcgtct acctcggctt caaggacgat accaacgccc
    17521 gcaacttcag cgggctcaac tttgacccca aggccatggc cgacggcgtg cgttacgccc
    17581 acgaccgcgg ccgcaaggtg ttgctggccc tcaacaccta tccccagacc gatacctggc
    17641 agcgctggac cggcgccgtg gaccgggccg ccgaactggg catcgacgcg gtgatcctcg
    17701 ccgatcccgg cctcatggcc tacgcccggc gcgtgcatcc cgagctgcgc ctgcatctgt
    17761 ccgtgcaggg ctcggccacc agctacgagg ccatcaactt ctaccacgcg cacttcggca
    17821 ttgcccgcgc ggtgctgccc cgggtgctgt ccctggccca ggtggagcag gtcatcgccc
    17881 acacgccggt ggagatcgag gtcttcggct ttggcggcct gtgcgtgatg gtggaggggc
    17941 gctgtgccct gtcggccttt gccaccggcg aatcgcccaa ctgcaacggc gcctgctcgc
    18001 cgggcaagca cgtgcgctgg gagcagaccc cccgcggcat ggagacccgc ctcaacggca
    18061 tcctcatcga ccgctttgcc gacggcgaac gcgccggcta tccgaccctg tgcaagggcc
    18121 gcttcgaggt gaacggcgag acctactacg ccatcgagga acccaccagc ctcaacaccc
    18181 tggagctcct gccggagctg gcccgcgtcg gcgtgcgtgc catcaagatc gaaggccgcc
    18241 agcgcagtcc cgcctacgtg gcccaggtga cccgcgtctg gcgcgccgcc atcgaccgcg
    18301 tgctggccgg cgcagggcct ttccgcgtcg aacctgcctg gatggccgag ctcaacaagg
    18361 tctccgaagg ccagagccac accctcggcg cctactaccg tccctggaaa taatcccgcc
    18421 tgtcgccccg atgaaactcg ccctcggccc cctgctgtac tactggcccc gcgccgccac
    18481 cctgtccttc tacgccgaga ttgccgaatc gccggtggac atcgtctacc tgggcgaaac
    18541 cgtctgttcc cgtcgccacg agctgcgcct cgcggactgg ctggaggtgg ccgagaccct
    18601 cgccgcgacc ggcaaggagg tcgtgctctc cagccaggtg ctggtggaat ccgaatcgga
    18661 cctgaagacc ctgcgtcgca tcgtggacaa cggccgcttc cgcgtcgaag ccaacgacat
    18721 gggcgccgta cgcctcctga ccgccggcga cggtgcgcgt cccgactgga tcgccgggcc
    18781 gaccctgaac atcttcaacc ccggcaccct gggcctcttc gccggcctgg gcgccagccg
    18841 ctgggtggcg ccgccggaac tgtcccgcga ccagatcgcc gccgtgcgcg cccagctggg
    18901 gctgcacatg gagaccgagg tcttcgccca tggccgcctg cccctggcgt attccgcgcg
    18961 ctgcttcact gcccgccact tcaacctgca gaaggacacc tgtgagttcc gctgcctgca
    19021 gttcgccgac ggcatccccc tcaagacccg cgaaggcgag cccttcctgt gcctcaacgg
    19081 catccagacc cagtccgccc acgtgcacac cctgctcggc gatctgcccg ccgtggttgc
    19141 cgacggcatc gacatcctgc gcatcagccc ccagggcgaa cacaccgccg aggtcgtgac
    19201 gctcttccgc gacgccctgg aggggctcac cccgccccag gacgccctgc aggaagccct
    19261 gcccctgttg ccggaagcgc cgtgcaacgg cttctggcac ggccgcgccg gcgtcgaaca
    19321 gttcgtcacc gcctgacctt taccggatcg gacctgccat gaagctgccc gccttcaccc
    19381 tgccgagcct cgtcacccgc gtcggcgccc gcctgccgca actgcccccg accctcgccc
    19441 tcgtcgccgg cctgaacgcc gccctcgacc gcctcatccc ccgcgacacc ctcgaacccc
    19501 tcaccggcaa gcgcctcctg ctgcgcatca ccgacgccgg cctcgccctg cgcttcaccc
    19561 tcaccccccg cggcttccgc cccgtcctgg accgccgcgc ccccgacctg acgatctccg
    19621 ctaccacccg cgactacctc gccctcgccc tccgcgaaga agaccccgac accctcttct
    19681 tcagccgccg cctgctgatg gaaggcgaga ccgacctggg gctgctggtg aagaacacgc
    19741 tggatgcggt ggattgggag gcggtgtggc gggtcgtgaa gtccccaatc gtgggcatga
    19801 cgggcgcgtc tcagaccgga tcaacagtca cgtaatacac gagcgtactg tccgggtcga
    19861 ccttgatcag gtcggcgctg gtgatgcctt ccgggatgtc tcgtccactc gggaatcgcg
    19921 ggaattgggt gttgttgatg aatctgtcga attgcctttt cccgcaatgg gggcagggcg
    19981 gcattgacga cagaaatcgg gcgattttgc cttcgtagta tctgcggtat tcgccgtggt
    20041 gagtggcaac ctgcgcgggc acagattcgc tgagaatcgg atgggttcgc gggttcgtcc
    20101 agagaatggt gtgacagtgg ctgcattggg caccggcaat gggtgtgtcg gcccagaaaa
    20161 aatacaccac cccgtcgttc tcgaagtttt tgatcagaaa tggctctagt ctgatcttgg
    20221 ctgcggcgac ttttttgatt ttaatttcag agcccggtct tgtatggggt tgttgtgggc
    20281 acggccgagg aaaatgtagg ggcgtgcgga gggtgtgatt tcagcctggc tcaatcggtg
    20341 agcgcgaatc tgaattctgc atagtctctt tccaagtccc tgtattttct cagggtgcct
    20401 tccgtgaagc actggctgcc agggatgtct ctccactcaa tcggagggtg gatttcaccg
//
LOCUS       DAOQPA010000059        20380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1291_length_20380_cov_15.513112, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000059 DAOQPA010000000
VERSION     DAOQPA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20380
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_20380_cov_15.513112"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="thrC"
                     /locus_tag="PKX01_09690"
     CDS             <1..1049
                     /gene="thrC"
                     /locus_tag="PKX01_09690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMZ76440.1"
                     /translation="FEYVLEARGEQINILGATSGDTGSAAEYAMRGKHGVRVFMLSPH
                     GRMSAFQRAQMYSLQDDNIVNIAVRGMFDDAQDIVKAVSNDHAFKAQYKIGAVNSINW
                     ARVAAQIVYYFKGYFAATASNDQQVAFVVPSGNFGNICAGHIARQMGLPVAQLVLATN
                     ENDVLDEFFRTGVYRPRASSETHATSSPSMDISKASNFERFVFDLVGRDGAKVRELWA
                     AVDAGGAFDLSATPAFARIGDYGFVSGRSSHADRLATIRLADARYATVIDTHTADGLK
                     VALERRDPAVPTLVLETAQPAKFEDTIVEALGRKPARPAGLENIEALPQRVQVMDVDA
                     AAIKALIAAAVDAA"
     gene            1054..1677
                     /locus_tag="PKX01_09695"
     CDS             1054..1677
                     /locus_tag="PKX01_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ76441.1"
                     /translation="MRPDDPRHGWNSRYADAGDDYVFGTAPNAFLAAQARWLTPGQRA
                     LSVADGEGRNGVWLAEQGLQVDAVEFAPAAIAKARRLAAARGVTPNFIEADLLTWDWP
                     VSAYDVVAAIFIQFVGPAERARLFACMTAALRPGGLLLIQGYTPRQLDYRTGGPSAVE
                     NLYTQALLRDAFAGLEILHLAEYDSHVDEGHGHRGTSALIDLIARKP"
     gene            complement(1765..3852)
                     /locus_tag="PKX01_09700"
     CDS             complement(1765..3852)
                     /locus_tag="PKX01_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76442.1"
                     /translation="MQHRFAPTTLAAALAALGLPAAHAAGDAPSVTLNPVVVTGSRAE
                     AASFDLPAAIDVLNREQLTAGQPRVNASEALISIPGVVANNRQNYAQDLQISSRGFGA
                     RSAFGVRGIKLVADGIPASMPDGQGQAATFNLDMAERMEVLRGPFSALYGNHSGGVIQ
                     YFTREPEAAAVSGGFAFGSYGTTKADLTVEGKEGATGAIANVSRLDTNGYRDHSAALR
                     EQAFAKIGTRLSDVSTLSFVVNGLWQHDTQDPLGLTWAAAKNAPRSVDASALTYNTRK
                     SIDHLQGGVTYDRRFGSDALQLTAYSGQRSVVQYQAIPISSNAKSSGGVVDFDRNFYG
                     FGARWIARGEALGGRVTTTTGVDYDASTDKRSGYDNNKGTASTLRRRESDSVAASAVY
                     VQSEWARDAWRLTGGLRYNTVKFDVYDEYLSNGNDSGSHDYSRATPVVGVVYALNPAV
                     NLYASAAKGFETPTLNELFYSAGGTGFNFGLAAARSQHLEVGAKALVGDASRVNVALF
                     QVSTDDELVVADSTGGRTSYKNAGKTLRQGIEVSAETAWQRDLRSRVSLSHLRAVYDE
                     AFNTVFQSKAITVPAGNAIPGVPRTTLFGDLAWTPVAGITAAVEGVYRSKVYVEDSNT
                     ATPASSYALANVRLTAEQRSGHWRFSEFARVDNLFDRQYIGSVIVGDSNSRYYEPSPG
                     RNGMVGVNLRYLW"
     gene            4152..5312
                     /locus_tag="PKX01_09705"
     CDS             4152..5312
                     /locus_tag="PKX01_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HMZ76443.1"
                     /translation="MKTAPHPLWLVGFRPFFVLACAAGAALPMLWALVFADTLTLPAG
                     RLSPLQWHAHEMLFGFGWAVLGGFLLTASKNWVQIRGYHGAALKALVAAWCVERIGIS
                     FGGTWPAALFWASAFAFLGAIVAMLVATLVRHRARDSFRDNLFFIVALPLFLVAKALV
                     LDADHFHAGVSMTLGLYRLAFLVMLERTLTTFMKNGCGVDLPRDPWLDGPIKGLALVL
                     VGLPWLPAGLAVALLLVEAVLLTARFFRWRPDRAFRRLELAVMYVGYLALIAQLLAEA
                     WSRIGAPGWVGSVPVHLLTVGAMGPIIPAMLVRISKGHTGRKVSFDAFDKAVLWVMLA
                     ALGLRIGLPQLAPGLYGLWVDLAAAAWGLGFGALGLRILPRVLAPRVDGKVH"
     gene            5451..6062
                     /locus_tag="PKX01_09710"
     CDS             5451..6062
                     /locus_tag="PKX01_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76444.1"
                     /translation="MQYPDFFDAVPRLRIRDPLAEFLGAADEGVLEYRYLDAVRLAGH
                     SCPTVAGAFWLTRLALGALYCTRMAVRGGVRVAFRERVDNGVTGVVANVVSMLTGAAA
                     DGGFKGLGGQFERRGLLGFGGEQPFELRYTRADTGISVDASVQLGQVPADPDMAPLMR
                     RCLAGEADADEARRFRELWQDRVRRLLFEHGDDPDVFVIRYVN"
     gene            6070..6576
                     /locus_tag="PKX01_09715"
     CDS             6070..6576
                     /locus_tag="PKX01_09715"
                     /inference="COORDINATES: protein motif:HMM:NF040052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HMZ76445.1"
                     /translation="MSSDDASRRGLASPPCYAAEFPGYFGDASLAPEVVARLNALLEA
                     ERAGAKVLRLLARELPDDASARALLESVCDDEGDNAVALYRTIRRLGGEASAVTGAFV
                     EKTLAIEGLEARLRFVNTGQQWVVRKIDEVLPLVHDGAARAMLDAMRQSHRDNIAACD
                     AFLATVHR"
     gene            complement(6597..7436)
                     /locus_tag="PKX01_09720"
     CDS             complement(6597..7436)
                     /locus_tag="PKX01_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HMZ76446.1"
                     /translation="MPRPHRLTATALILALACAGFVPVRADPPPVPTASAGSEDEAMQ
                     PKFVWGILIKIAYNVASSVFSDWAKSKLSIDLSPGGLAKLFFNSSTARIVPVSLPSLG
                     FLAKQADGVGNAVAGEPTAPLVVNAQGINYQGVHVALLGFDRAGKALGYRPVTDGFRS
                     GERFKLRILPTFDGLLALDNINPQGRRAQVYPPERGTMVEVKAGVEILVPLDRDQYLE
                     FAGSTGEEKLVVTLRSPRAVGDAASPATVYRHDEAIGSSFVQETRPGTYPVIVQALSL
                     RHD"
     gene            complement(7475..9076)
                     /locus_tag="PKX01_09725"
     CDS             complement(7475..9076)
                     /locus_tag="PKX01_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ76447.1"
                     /translation="MPRLPSAPAALRAVACLALATVLLAPVCRAADAPAADSGFARPP
                     SPHVHALILTIGAYRNGIPPLTGVARDADNAREIARRMGVPPAHITVLRDAELTLDGL
                     RRAFDQLDGRVRDGDEVFVYYSGHGGRQKVVEAGVERCAESLITVDGYGFTDAELETR
                     LKALSTRAGKMVVFLDACHSGGVTTRATRVDPPFLPKYWQGKAGGGDACARPVNVLTR
                     DIVLATATPGSGGANYAYIAAARDNEISLDQPGKGGLATQAWLACLAGQARDTDGSGG
                     LSAEEIRVCAQQHIDAKLKNAEGFLPHHVAITGNRDMVLAYDTKAGAAALPAVLPSPS
                     ATASPTPAAPTAPVAVPPAVPTALPLKASPLATLHDLYANRDDRRLVQLATATPRVRI
                     GRDEVRFTLSSREGGYVYLLMVGSNGEAFDLLFPNALDRDNRLRPGETLTLPRRSWQI
                     GAGGPAGTDTLLALVTDSPRDFRGLGLKPVGPFSAIEPTRARDIQLVTAHATPPADCP
                     RDLAAQPACSASYGAALLSIEEVAP"
     gene            complement(9110..10852)
                     /locus_tag="PKX01_09730"
     CDS             complement(9110..10852)
                     /locus_tag="PKX01_09730"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ76448.1"
                     /translation="MTSRRRFVQSLAALSVGALPRPLPAATDPSRQALVIGNAAYRFS
                     PLTNPTNDARAVSELFGRAGFTVSTHLDVGQRDMLAAIEQFGQVVRRSDTRLVVFYYA
                     GHGAQLDWRNYLLPVDAAVQTPEQMRQRCVDLNTLLGRIAGSSGKTFVIILDACRNNP
                     FGSAYTPEQKGLSQFDAPPGSLLAYATSPGNVASDGSGRNGLYTENLVRELSVPGARI
                     EDALKRVRLNVRLASRGAQVPWETTSLENDVILFDTGHARLSEAEQEKQLEEDIARWN
                     AIKNSRNADDWVAYLRAFPNGRFAEIAQMRLIRLLAEADGGRPANPAAAAVTPAVSAP
                     APMPAPASAPPTVPTDTEAAPAIVLGAGLPVPVLIAPSGNPFSSGRYPLARHFAPGDS
                     ATFRKSDLLTGLVDKTWTVRVNRVDRDADRVELNRGSWIVDLMGNTLHSDNVDYDAPV
                     AWTPSEFYVGKKWTLAYRRNENGTVSTGTYAVQIVARETITVPAGTFDTFRIEGEGWS
                     ATFGSRLQSKFWLVPSLNFLVRSEVMNRNRRGQFTVTERRELVALRQGGIDTSCAAPG
                     PSGTRTLVIRNNCG"
     gene            complement(10961..12334)
                     /gene="chrA"
                     /locus_tag="PKX01_09735"
     CDS             complement(10961..12334)
                     /gene="chrA"
                     /locus_tag="PKX01_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMZ76449.1"
                     /translation="MTPSSSPDTAQPPAQPADAAFWPAFRFWLKLGFISFGGPAGQIA
                     LMHRELVERRRWISEGRFLHALNFCMVLPGPEAQQLATYLGWLMHRTPGGLAAGVLFV
                     LPAFFILVGLSWLYVAWGQVPWVAGLFYGIKPAVTAIVAQAAWRIGGRTLKNRALGAI
                     AAASFIAIFALGLPFPAIVAAAALIGWLGGRLAPELFRTGGGHGPAAAQAAPALIDDD
                     TPTPAHARFTPGRLLRVVLFGGLLWALPMALLGVAAGWDGPLTQMGRFFTQAALLTFG
                     GAYAVLPYVFQGAVAHYGWLSAAQMMDGLALGETTPGPLIMVVTFVGFVGGAAHTVLG
                     PDNPFLSGLAAAAVVTWFTFLPSFVFIFAGGPFIETTHNDLHFTAPLTAITAAVVGVI
                     LNLALFFCAHVLWPQGLTGGALAGFDGIAALLAAGAAVALFRFKVGVIPVIGACAVLG
                     GLLSLLR"
     gene            13005..13385
                     /locus_tag="PKX01_09740"
     CDS             13005..13385
                     /locus_tag="PKX01_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76450.1"
                     /translation="MATSSHTRVAPTQLDTDVDTYSALRGLVGYTPRNARFSTESAGA
                     ALAAMRAADEAAIQANNALTVARESLIAAQKDFHEIILGVKAEAKVIFGEDSDEVAAL
                     GLKRKSDRAGPKRSSKPTPPTKES"
     gene            13532..14047
                     /locus_tag="PKX01_09745"
     CDS             13532..14047
                     /locus_tag="PKX01_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76451.1"
                     /translation="MIDLEMQLRRADRLDAITKSLGFALWQLQALEETTANYYVLVAL
                     ATRGMGIEAGRKLDDSVKGNTFGKTIHALRDSGKMPSKIEQRFLALLKERNWLVHKSR
                     ATNRGAVHDNQACDSLLVRLDQLAEEARLLLREIGKASEKFVVQSGVPIEEVDRLTHE
                     ILNSWHSTTNE"
     gene            14238..14837
                     /locus_tag="PKX01_09750"
     CDS             14238..14837
                     /locus_tag="PKX01_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76452.1"
                     /translation="MAETDRDLGVAAAAIEAMASTKKLSEFKSFWESFLFRLERAWER
                     NQRRVQTVPGRDAKSWLSTNAKLRRSDPLLNYLKQARNAEVHAISSSIESELIVSVAD
                     RFGRPFSMKDMSLSIEGATFVIDLTSTDIGLEWYGDVALSEPKLQCVINRGKRYDPPT
                     RHLGNAIQNHHPVAISILGLNFYKGTYAALGALLNHKKP"
     gene            complement(15102..17264)
                     /locus_tag="PKX01_09755"
     CDS             complement(15102..17264)
                     /locus_tag="PKX01_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ76453.1"
                     /translation="MSHALRHPLPAEGIPGRRPSAPTPASRWRGLAGVLSLLLAACAL
                     FAPRLADAGDLTRDDIARRFQPPLHVGDKLRDIPAWPITSELEPEAGPVAYAFESIDL
                     APIPGFEGTPYNLLVSIDRKGNFLGVELLRQHEPVFLSGLGEGPLKDFLRQYEGKSLK
                     QEITVSSLYGNTRSGAGARRVVLDGVSKATASVRIANQTVLTAALAVARARLGFAAPV
                     DKGPTAEARADLYEARSFAQLVDDGAIGRLHLRNADVEKLFAGTDAAGLDADALARPD
                     DTFTDLYVAYLNAPTIGRALLGDAGHADLMRRLEPGQHAWWVATAGRDAFIDDQFTRG
                     TVPVRLAFSQAGGPVELRDLDLSPKPPAGAPPLNAALVLRVPPMSGVDPASPVRFELT
                     LNRSKGSILPQTFQKTVALDYQPPERLFVRPPRPLPEWLVAWKDRAAELIVIGLALAG
                     LTVVLARPRWMSISAPRLRVFRLGFLAFTLVYLGWYAQGQLSIVQITGAVKSLAAGQG
                     LKSFLYDPVSLLLIAFTAVSFVVWGRGTFCGWLCPFGALQEFAALLARALRLPALRMP
                     ARLGLVLERSRYAVLAALVLAAAFAPRLAEQGVEIEPFKTAITVGFNRDGPYVAWAVA
                     LLIAGGLYYKFFCRYLCPLGAAMTLGGRLRLLDWLPRRAECGQPCQTCRHRCTYDAIE
                     KSGAIRYDRCFQCLDCVGIYHDPGRCAPVMLVRKKAGR"
     gene            complement(17277..17807)
                     /locus_tag="PKX01_09760"
     CDS             complement(17277..17807)
                     /locus_tag="PKX01_09760"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ76454.1"
                     /translation="MHPLSRIALASLAALFTPLIAAPALAARPVLHVKESIVIAAPPA
                     QVWAAAGDFGNLGWHPAVASTTIDQGENNRPGAVRTVTTKDGAKLVERLVARDDAARR
                     LSYTIIDSPLPVSDYRSTLRVLPDGAGSRVEWSSRFRRKAEKPLDGADDAGARRVIHS
                     IYSGGLGNLKATLEAH"
     gene            complement(17830..18471)
                     /locus_tag="PKX01_09765"
     CDS             complement(17830..18471)
                     /locus_tag="PKX01_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76455.1"
                     /translation="MTPELSARTVLLADDHAIVRMGFRLLLEGVGVHIVGEAESGEEA
                     QRLAAELRPDVLVMDLSMPGCGGLAALERILARSPAQAVLIASAHADAVTARRALKLG
                     ARGYLCKRSPPGELLTAVARIAAGQTYIDPGIAPELARGDKSADPAEALTDKEFHVFL
                     QLARGRSVNEVAATFHLSPNTVGTHLYHIKQKLGVQNMAELAMAAVRAGLIEV"
     gene            complement(18461..19903)
                     /locus_tag="PKX01_09770"
     CDS             complement(18461..19903)
                     /locus_tag="PKX01_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ76456.1"
                     /translation="MNTASPTAAPGARTAPTPDATTRALRWPRPRRLSLRLRISLILT
                     AFAALLILAGGALWIAEARNAIAEEVTSAHRVAVQWLTVSARAASDNDPAWSEPRLVA
                     YLQAAGRLRANQVELRDARGALLYRSPGSPYKAGRDAPPLFVAWMSPDLPARSLPAGH
                     LDIVIQPDASRAVLDLWDDLTAAAGRALLALIALFAGCWLAVDRALRPLGQVMAALGH
                     AGDGRFDTRLPEDGPRELARLAEAFNRMTLRLDQAVADNARLHHEQALGHVLHQRLEA
                     ERTVIARELHDELGQSITAVAALAGAILQRSDGQPTVQQSARTIRDVAAHMQDDVRAL
                     LTRLRPQRPAEETLDDALRSVLGAWQQRHPDIRLVTELFAGPAPLGDELTLVALRIVQ
                     ESCTNIIRHAQASQAYVRVLRQAGRLVVEVGDNGRGLQPASGQSGYGLTGMRERVAAV
                     RGELHISSPPGGGTRIRAVLPLPDSAPHDA"
     gene            complement(19900..>20380)
                     /locus_tag="PKX01_09775"
     CDS             complement(19900..>20380)
                     /locus_tag="PKX01_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ76457.1"
                     /translation="PTRAAAPDTPVIVQAPTPPVPSSAPISAPAEASPRPAPVVAVTT
                     PAEAAPAPHVDEAPDPALLERYGRSLSGLLARQQNYPRLAALRGWEGEVQLRVTIARK
                     GTIVATQVLRSSGFEVLDQNAIALVTAAAPLPRVPDALQGREFQIVVPVQYRLDKQS"
BASE COUNT         3088 a         6341 c         7772 g         3179 t
ORIGIN      
        1 tcttcgagta cgtgctggag gcccgcggcg agcagatcaa catcctcggc gccacgtctg
       61 gcgacaccgg ctcggcggcc gagtacgcca tgcgcggcaa gcacggcgtg cgtgtcttca
      121 tgctctcgcc ccacggccgc atgagcgcct tccagcgcgc ccagatgtat tcgctgcagg
      181 acgacaacat cgtcaatatc gccgtgcgcg gcatgttcga cgacgcccag gacatcgtga
      241 aggcggtctc caacgaccac gccttcaagg cgcagtacaa gatcggcgcg gtcaattcca
      301 tcaactgggc gcgggtcgcg gcgcagatcg tctattactt caagggctat ttcgcggcca
      361 ccgcgtccaa cgaccagcag gtcgcctttg tggtgccgtc gggcaatttc ggcaatatct
      421 gcgccggcca catcgcccgc cagatgggcc tgccggtggc ccagctggtc ctcgccacca
      481 acgagaacga cgtgctcgac gagttcttcc gcaccggcgt gtatcgtccc cgcgccagca
      541 gcgagaccca cgccacctcc agcccctcca tggacatctc caaggcctcc aacttcgagc
      601 gcttcgtctt cgacctggtc ggccgcgacg gcgccaaggt gcgcgagctg tgggccgcag
      661 tggatgcggg gggcgccttc gacctgtccg ctacgccggc cttcgcccgg atcggcgact
      721 acggcttcgt ctccggccgc agcagccatg ccgaccgtct ggccaccatc cgcctggccg
      781 acgcccgcta cgccacggtg atcgacaccc acaccgcgga cggcctcaag gtggcgctgg
      841 aacggcgcga tccggcagtg ccgaccctgg tgctggaaac cgcccagccg gccaagttcg
      901 aggacaccat cgtcgaggcc ctgggccgca agccggcgcg tcccgccggc ctggagaaca
      961 tcgaggccct gccccagcgg gtgcaggtga tggacgtgga tgcggcggcc atcaaggcgc
     1021 tcattgccgc cgcggtggac gcggcctgat ccggtgcgtc ccgacgatcc ccgccacggc
     1081 tggaacagcc gctacgccga cgctggcgac gactacgtct tcggcaccgc gcccaacgcc
     1141 ttcctggcgg cccaggcgcg ctggctgacg cccggccagc gcgccctgtc ggtggcggac
     1201 ggggagggac gcaacggcgt atggctggcc gagcagggcc tgcaggtgga tgcggtggag
     1261 tttgcccccg cggccatcgc caaggcccgc cgcctggccg cggcgcgggg cgtgacgccg
     1321 aacttcatcg aagccgacct gctcacctgg gactggccgg tgtcggctta cgacgtggtc
     1381 gcggcgatct tcatccagtt cgtcggtccg gccgagcggg cgcggctctt cgcctgcatg
     1441 acggcggcgc tcaggccggg cgggctgctg ctgatccagg gctacacccc gcgccagctc
     1501 gactaccgca ccggcggccc gtcggcggtg gaaaacctct acacccaggc cctgctgcgg
     1561 gacgccttcg ccgggctgga gatcctgcac ttggcggaat acgacagcca tgtggacgaa
     1621 ggccacggcc accgtggcac ctccgccctc atcgacctga tcgcccgcaa gccctgaggg
     1681 gcgggacgtc caacaaaaaa ccggccacgc aggccggttt tttgttgtcg ggtcgcgccg
     1741 tcacaggccc ggcgcccgcc acactcacca caggtagcgc agattcaccc ccaccatgcc
     1801 gttgcggccg ggggagggtt cgtaatagcg gctgttgctg tcaccgacga tgaccgagcc
     1861 gatgtactgg cggtcgaaga ggttgtccac ccgcgcaaat tcgctgaagc gccagtggcc
     1921 gctgcgctgc tcggcggtga ggcggacgtt ggccagggcg taggacgagg ccggcgtggc
     1981 ggtgttggaa tcctccacgt agaccttgct gcgataaacc ccttccaccg ccgcggtgat
     2041 gcccgccacc ggcgtccacg ccagatcgcc gaagagggtg gtgcgcggca cgccggggat
     2101 ggcgttaccg gccggcaccg tgatggcctt gctctggaag accgtgttga aggcctcgtc
     2161 gtacaccgcg cgcaggtggg acagggacac gcggctgcgc aggtcgcgct gccatgcggt
     2221 ctcggcggat acctcgatgc cctggcgcag ggtcttgccg gcgttcttgt agctggtgcg
     2281 accgccggtg gagtcggcca ccaccaactc gtcgtcggtg ctcacctgga acagggccac
     2341 attgacgcgg ctggcgtcgc ccaccagggc cttggcgccc acttccaggt gctggctgcg
     2401 ggcggcggcc aggccgaagt tgaagcccgt gccgccggcc gagtagaaca gttcgttgag
     2461 ggtgggcgtc tcgaagccct tggcggcgct ggcgtagagg ttgaccgccg gattcagcgc
     2521 atacaccacg cccaccaccg gggtggcgcg gctgtagtcg tggctgccgc tgtcgttgcc
     2581 gttagacagg tattcgtcgt agacatcgaa cttgacggtg ttgtagcgca ggccgccggt
     2641 caggcgccag gcgtcccgcg cccattcgct ctgcacgtac acggcggagg cggccactga
     2701 gtcgctctcg cggcgacgca gggtggaggc ggtgcccttg ttgttgtcgt agccgctgcg
     2761 cttgtcggtg ctggcgtcgt aatccacgcc ggtggtggtg gtcacgcggc cgcccagggc
     2821 ctcgccgcgg gcgatccagc gggcgccgaa gccgtagaag ttgcggtcga aatccaccac
     2881 gccgcccgag ctcttggcgt tgctggagat gggaatggcc tggtactgca ccacgctgcg
     2941 ctggccgctg taggcggtga gctgcagggc atcgctgcca aagcggcggt cgtaggtgac
     3001 gccaccctgc aggtggtcga tgctcttgcg ggtgttgtag gtgagagcgg aggcgtccac
     3061 gctgcgcggg gcattcttgg cggcggccca ggtgaggccc aggggatcct gggtgtcgtg
     3121 ctgccacagg ccattcacca cgaagctgag ggtgctcacg tcgctcaggc gggtgccgat
     3181 cttggcgaag gcctgctcgc gcagggccgc gctgtggtcg cgatagccgt tggtatcgag
     3241 acgcgagacg ttggcgatgg cgccggtggc gccttccttg ccttccacgg tgaggtcggc
     3301 cttggtggtg ccgtagctgc cgaaggcgaa gccgccggag accgccgcgg cttccggttc
     3361 gcgggtgaag tactggatca cgccgccgga gtggttgccg tagagggccg agaagggccc
     3421 gcgcagcact tccatgcgct cggccatgtc caggttgaag gtggcggcct gaccctggcc
     3481 gtcgggcatg ctggcgggga tgccgtcggc caccagcttg atgccgcgca ctccgaaggc
     3541 agagcgggcg ccgaagccgc gggacgagat ctgcaggtcc tgggcgtagt tctggcggtt
     3601 gttggcgacg acaccgggga tcgagatgag ggcttcggac gcgtttaccc ggggctggcc
     3661 ggcggtgagc tgctcccggt tgaggacgtc gatggcggcg ggcaggtcga agctggcggc
     3721 ttcggcgcgg ctgccggtga cgaccaccgg attgagggtg acggacgggg cgtcgccggc
     3781 cgcgtgggcg gcgggcaggc ccagagcggc gagggcggcg gcgagggtcg tcggggcaaa
     3841 gcggtgctgc atggggcgtc tccgtgtttc tggctgttct gatccccggg aggtggccgg
     3901 gagcggcgca acgatagcct gattccacgc cgcgcgcctc acgaaaatca tgagatgggc
     3961 gtcagccgga ggttttctgc atggggcggg gaaggcgtcg cgggtcgatg gggctgcgtc
     4021 cgtgttgggg acggccggaa cggtgggtgg cgcgcggtgg cctggacggg tgccgcctcg
     4081 gcctgccctc gatcaaggcg cgcgccggcc gctgccgcta tgctggccct tttccgcatt
     4141 gcgccgcacc catgaagact gccccgcatc ccctctggct ggtgggattc cgtcccttct
     4201 tcgtccttgc ctgcgccgcc ggtgcggcgc tgcccatgtt gtgggcgctg gtatttgccg
     4261 acacgctgac gctgccggcc ggccgcctgt caccgctgca gtggcatgcc cacgagatgc
     4321 tcttcggctt cggctgggcg gtgctcgggg gctttctgct gaccgcctcg aagaactggg
     4381 tgcagatccg cggctatcac ggcgcggccc tgaaggccct ggtggcggcg tggtgcgtgg
     4441 agcggatcgg catcagcttt ggcggaacgt ggccggcggc cctgttctgg gccagcgcct
     4501 tcgccttcct cggcgccatc gtggcaatgc tggtggccac cctggtacgc caccgggcgc
     4561 gggacagctt ccgtgacaac ctgttcttca tcgtcgcgtt gcccctgttc ctcgtcgcca
     4621 aggcgctggt gctggacgcg gaccacttcc acgcgggcgt gagcatgacc ctgggcctgt
     4681 accggctggc ctttctggtg atgctggagc gcaccctgac gaccttcatg aagaacggct
     4741 gcggcgtcga cctgccgcgg gatccgtggc tggacgggcc gatcaagggc ctggcgctgg
     4801 tcctcgtcgg cctgccctgg ctgcccgcgg ggctggcggt ggcgctgctg ctcgtcgagg
     4861 cggtcctgct cacggcccgc ttcttccgct ggcgcccgga ccgggccttc cgccgcctgg
     4921 agctggcggt gatgtacgtg ggctatctgg ccctgatcgc ccagctgctg gccgaggcgt
     4981 ggagccgcat cggtgcgccg ggctgggtgg gcagcgtgcc ggtgcatctg ctcacggtgg
     5041 gggcgatggg cccgatcatt ccggccatgc tggtgcggat ctccaagggt cacacggggc
     5101 gcaaggtgag cttcgatgcc ttcgacaagg ccgtgctgtg ggtgatgctg gcggcgctgg
     5161 ggctgcgcat cggcctgccg cagctcgcgc cggggctcta cgggctgtgg gtcgatctgg
     5221 cggcggcggc ctggggactc ggcttcggcg cgctggggct gcgcatcctg ccccgggtgc
     5281 tggcgccccg ggtggatggc aaggtgcatt gaggggctca gggtttaccc gtaggaaaaa
     5341 tgtgccagac gtgagattga tcaaggtttt ctatggaagg atcgaatacc cttctgggct
     5401 tggggcggtg agaccagaac attcagccgc ccggcagtga gggagcgtgt atgcagtacc
     5461 cggatttttt cgatgccgtg ccgcgcctgc ggatacggga cccgctggcg gaatttctcg
     5521 gcgcagcgga cgagggcgtc ctcgaatatc gatacctcga cgcggtgcgt ctggccggac
     5581 attcctgccc gaccgttgcc ggcgccttct ggctcacccg tctggccctc ggcgccctgt
     5641 attgcacccg catggcggtg cggggcgggg tgcgggtggc gtttcgcgag cgcgtggaca
     5701 acggtgtcac cggcgtggtg gccaatgtgg tgtccatgct caccggcgcg gcggcggacg
     5761 gaggcttcaa gggcctgggc ggccagttcg agcgtcgcgg cctgctcggc ttcggcggcg
     5821 agcagccctt cgagttgcgc tacacccgcg cggacacggg catctcggtg gatgcgtcgg
     5881 tgcagctcgg gcaggtgccg gccgatccgg acatggcgcc gctgatgcgc cgctgcctcg
     5941 cgggggaggc cgacgcggac gaggcgcggc gcttccgcga gctgtggcag gaccgggtgc
     6001 gccgcctgct gttcgagcac ggcgacgatc cggacgtgtt cgtgatccgc tacgtgaatt
     6061 gaggcggcca tgagcagcga cgacgcctcc cgccgcggcc tggcctcgcc gccctgttat
     6121 gcggcggagt ttcccggcta tttcggtgac gcgtccctgg cgcccgaagt cgtcgcccgc
     6181 ctcaacgccc tgctcgaagc ggagcgcgct ggcgccaagg tgctgcgcct gctggcgcgc
     6241 gagcttccgg acgacgcgtc ggcgcgcgcg ctcctcgaaa gcgtctgcga cgacgagggc
     6301 gacaacgcgg tggccctgta ccgcacgatc cgccgcctgg ggggcgaggc ttcggcggtg
     6361 accggcgcct ttgtcgagaa gaccctcgcc atcgaaggcc tggaggcgcg tctgcgcttt
     6421 gtgaataccg gccagcagtg ggtcgtgcgc aagatcgacg aagtgctgcc cctggtgcac
     6481 gacggcgctg cccgggcgat gctggacgcc atgcgccagt cccatcggga caacatcgcc
     6541 gcctgcgacg cctttctggc caccgtccac cgctgatccg gcgtccccgg cggcgctcag
     6601 tcgtggcgca ggctcagggc ctgcacgatc accgggtagg tgccgggccg cgtctcctgc
     6661 acgaagctgg aaccgatggc ctcgtcgtgg cgatacacgg tggccgggga ggccgcgtcg
     6721 cccaccgcgc ggggtgagcg cagggtgacc accagctttt cctcgccggt gctgccggca
     6781 aattccaggt actggtcgcg atccagcggg acgaggatct ccacgccggc cttcacctcc
     6841 accatggtgc ctcgttccgg cggatagacc tgggcgcggc ggccctgggg attgatgttg
     6901 tccagggcca gcaggccgtc gaaggtgggc aggatgcgca gcttgaagcg ctcgcccgag
     6961 cggaagccat ctgtcaccgg ccggtagccc agcgccttgc cggcccggtc gaagcccagc
     7021 agggccacat gcacgccctg gtagttgatg ccctgggcgt tcaccaccag cggcgctgtc
     7081 ggctcgccgg ccactgcgtt gccgaccccg tccgcctgtt tggcgaggaa gccgagggac
     7141 ggcagggaca ccggcacgat gcgcgcggtg ctgctgttga agaagagctt ggccagcccg
     7201 ccgggggaga ggtcgatgct cagcttgctc ttggcccagt cggaaaagac cgagctggcc
     7261 acgttgtagg cgatcttgat gagaatgccc catacgaact tgggctgcat cgcctcgtcc
     7321 tcgctgccgg cagacgcggt gggcacgggc ggtggatcgg cgcggaccgg cacgaagccg
     7381 gcgcaggcca gggcgaggat cagggcggtg gcggtgaggc ggtgagggcg cggcatgaag
     7441 gcgttccgat gggaggtggg cgagggtggg cgcttcaggg cgccacttcc tcgatgctga
     7501 gcagggccgc gccgtaggac gcggagcagg ccggctgggc ggccagatcc cgtgggcagt
     7561 cggcaggggg cgtggcgtgg gcggtgacga gctggatgtc ccgcgcgcgg gtcggctcga
     7621 tggccgagaa ggggccgacc ggcttgaggc cgaggccgcg gaaatcccgt gggctgtccg
     7681 tcaccagggc gagcagggtg tccgtgcccg ccggcccgcc ggcgccgatc tgccagctgc
     7741 gccgtggcag cgtcagggtc tcgccgggcc gcaggcggtt gtcccggtcc agcgcgttgg
     7801 ggaagagcag gtcgaaggcc tcgccgttgg agccgaccat cagcaggtag acgtaaccgc
     7861 cttcccgcga gctcagggtg aagcgcactt cgtcgcggcc gatgcgcacc cgcggcgtgg
     7921 cggtggcgag ctgcaccagg cgacgatcat cccggttggc gtacaggtcg tgaagggtgg
     7981 ccaggggcga agccttgagg gggagggcgg ttggcacagc gggcggcaca gcgaccggcg
     8041 ccgtgggcgc tgccggggtg gggctggcgg tggcggaggg gcttggcagc acggcgggga
     8101 gcgccgcggc gcccgccttc gtgtcgtagg ccagcaccat gtcccggttg ccggtgatgg
     8161 ccacgtggtg gggcaggaag ccctcggcgt tcttcagttt cgcgtcgatg tgttgctggg
     8221 cgcagacgcg gatctcttcg gcggagagcc cgccgctgcc gtccgtgtcc cgcgcctgac
     8281 cggccaggca ggcgagccag gcctgggtgg cgaggccgcc tttgccgggc tggtcgaggg
     8341 agatctcgtt gtcgcgggcg gcggcgatgt aggcgtagtt ggcgccgccg ctgcccggcg
     8401 tggcggtggc cagcacgatg tcccgggtga gcacgttgac cggtcgcgcg caggcgtcgc
     8461 cgccgccggc cttgccctgc cagtatttgg gcaggaaggg cggatcgacg cgcgtggcgc
     8521 gggtcgtcac gccgcccgaa tggcaggcgt cgaggaagac caccatcttt ccggcgcggg
     8581 tcgacagggc cttgaggcgg gtttccagct ccgcgtcggt gaagccgtag ccgtccacgg
     8641 tgatgaggga ttcggcgcag cgctcgacgc cggcctccac gaccttctgg cgaccgccgt
     8701 ggccggagta atagacgaag acctcgtcgc cgtccctgac ccgcccatcg agctggtcga
     8761 aggcgcggcg caggccgtcg agggtcagct cggcgtcccg cagcacggtg atgtgggccg
     8821 gcggcacgcc catgcggcgg gcgatctcgc gggcgttgtc ggcgtcccgc gccaccccgg
     8881 tgaggggcgg gatgccgttg cggtaggcgc cgatggtgag gatcagcgca tgcacgtggg
     8941 gcgaaggcgg gcgggcgaag ccgctgtcgg cggcgggagc gtcggcggcg cggcagacgg
     9001 gcgccaacaa cacggtggcc agagcgaggc aggccacggc gcgtagggcg gccggggcgg
     9061 aagggaggcg gggcatggcg gggatcacgg tgtcggtggg cgcgcggcgt cagccgcagt
     9121 tgttacggat caccagggtg cgggtgccgc tcgggccggg ggcggcgcag ctggtgtcga
     9181 tgccaccctg gcgcagggcc accagttcgc gccgctcggt cacggtgaac tggccgcggc
     9241 ggttgcggtt cattacctcg gagcggacca ggaagttgag gctgggcacc agccagaact
     9301 tggattgcag gcggctgccg aaggtggcgc tccagccttc gccctcgatg cggaaggtgt
     9361 cgaaggtgcc ggccggcacg gtgatggtct cccgcgccac gatctgcacg gcgtaggtgc
     9421 cggtggacac ggtgccgttc tcgttgcggc ggtaggcgag ggtccacttc ttgcccacgt
     9481 agaactccga gggcgtccac gccaccggcg cgtcgtaatc cacgttgtcg ctgtgcaggg
     9541 tgttgcccat caggtccacg atccaactgc cccggttcag ttcgacccgg tcggcgtccc
     9601 gatccaccct gtttacgcgc acggtccagg tcttgtccac caggccggtg aggaggtcgg
     9661 acttgcggaa ggtggcggaa tcgcccggcg caaagtggcg ggcgaggggg tagcggccgg
     9721 acgagaaggg gttgccggag ggggcgatca gcaccggcac cggcaggccg gcgccgagga
     9781 cgatggcggg ggcggcttcg gtgtcggtcg gcaccgtcgg aggtgcggac gctggggcag
     9841 gcatcggtgc aggcgcggac accgccggcg tgaccgccgc cgcggctgga ttcgccgggc
     9901 gcccgccgtc cgcttcggcc agcaggcgga tcaggcgcat ctgggcgatc tcggcgaagc
     9961 gcccgttggg gaaggcgcgc aggtaagcca cccagtcgtc cgcgttgcgg gaattcttga
    10021 tggcgttcca gcgggcgatg tcctcctcca gctgcttctc ctgctcggct tcgctgagcc
    10081 tggcgtggcc ggtgtcgaag aggatcacgt cgttttcgag ggaggtggtc tcccagggca
    10141 cctgggcgcc gcgggaggcc aggcgcacgt tgaggcgcac ccgcttgagg gcgtcctcga
    10201 tgcgggcgcc gggcacggag agttcgcgca ccaggttttc ggtgtacagg ccgttgcgcc
    10261 cgctgccgtc cgaggccacg ttgccgggcg aggtggcata ggcgagcagg ctgccgggcg
    10321 gcgcgtcgaa ctgggagaga cccttctgct cgggcgtgta ggcgctgccg aaggggttgt
    10381 tgcggcaggc gtcgaggatg atcacgaagg tcttgccgct gctgccggcg atgcgcccga
    10441 gcagggtgtt caggtccacg cagcgctggc gcatctgctc cggcgtctgc accgcggcat
    10501 ccaccggcag caggtagttg cgccagtcga gctgggcgcc gtggccggca tagtagaaga
    10561 ccaccagccg ggtgtcgctg cggcggacca cctggccgaa ctgctcgatg gccgccagca
    10621 tgtcgcgctg gcccacgtcc aggtgggtgc tcaccgtaaa accggcgcgg ccgaacagct
    10681 cgctcacggc gcgggcgtcg ttcgtggggt tggtgagggg cgagaagcgg taggcggcgt
    10741 tgccgatcac cagggcctga cggctcgggt cggttgccgc cggcagcgga cggggcaggg
    10801 cgcccacgga cagggcggca aggctttgaa cgaagcggcg acgggacgtc atggtgcggg
    10861 cacacggaac ggacagactc ggaccccgag cataacggct gcggccgacg gatcaagagg
    10921 gcgggcgcgg cggtgcgtgg tgggcgtcgc gtccgggcgt tcagcgcagc agcgacagca
    10981 gcccgcccag gaccgcgcag gcgccgatca ccgggatcac ccccaccttg aagcggaaca
    11041 gggcgacggc cgcgcccgcc gcgagcagcg ccgcgatgcc gtcgaagccg gccagcgcgc
    11101 cgcccgtgag accctgaggc cacagcacgt gggcgcagaa gaacagggcc aggttgagga
    11161 tcacccccac cacggcggcg gtgatggcgg tgaggggcgc ggtgaagtgc aggtcgttgt
    11221 gggtggtctc gatgaagggg ccgccggcaa agatgaagac gaaggacggc aggaaggtga
    11281 accaggtcac caccgcggcc gccgcgagcc cgctcaggaa ggggttgtcc ggccccagca
    11341 cggtgtgggc ggcgccgccc acgaagccga cgaaggtcac caccatgatc agcgggccgg
    11401 gcgtggtctc gcccagcgcc aggccgtcca tcatctgcgc ggcgctgagc cagccgtagt
    11461 gggccaccgc gccctggaac acatagggca gcaccgcgta ggcgccgccg aaggtgagca
    11521 gcgccgcctg ggtgaagaag cggcccatct gggtgagggg cccatcccag cccgcggcga
    11581 cgcccagcag ggccatcggc agcgcccaca ggaggccccc gaacagcacc acgcggagca
    11641 gccgccccgg cgtgaagcgg gcgtgcgccg gcgtgggcgt gtcgtcgtcg atgagggcag
    11701 gcgcggcctg ggcggcggcc ggaccatgcc cgcccccggt gcggaacagc tccggcgcga
    11761 gacgtccacc cagccagccg atcagcgccg ccgcggcgac gatggccgga aagggcagcc
    11821 ccagcgcaaa gatggcgatg aaggacgccg ccgcgatggc gcccagcgcc cggttcttca
    11881 gcgtccgccc gccgatgcgc caggccgcct gggccacgat ggcggtgacc gccggcttga
    11941 tgccgtagaa cagccccgcc acccagggca cctgtcccca ggccacgtag agccaggaca
    12001 gtcccaccag gatgaagaaa gccggcagca cgaagagcac ccccgcggcg aggccccccg
    12061 gcgtgcgatg catcagccag cccaggtagg tggccagctg ctgggcctcc gggccgggca
    12121 gcaccatgca gaagttgagg gcgtgcagaa agcgcccctc cgagatccag cgccgccgtt
    12181 ccaccagttc tcgatgcatc agcgcaatct gccccgccgg ccccccaaag ctgatgaagc
    12241 ccagcttgag ccagaaacgg aaggccggcc agaaggcggc gtcggcaggc tgcgcaggcg
    12301 gctgcgccgt gtccggcgaa gaggagggcg tcatgggggc gggctccgga agacgggggc
    12361 gagggggcag ggaacggcac tcggccggca cccgtgcctc accgccgcac tgtagagcat
    12421 gttggtgcgc cgcggcgggc gaaggtgggg ccgccgatgc agcctccgga cgaccccagg
    12481 cctccccgct gcgcgaagga ttcacacccc cgtgagaagg attcgcacgc ctgcgtgaag
    12541 gattcgcaag gctgtgggaa ggattcacag acgtgtgtga aggattcgca aggctgcgag
    12601 aaggattcac agccctgcgt gaaggattcg caaggctgcg tgtaggattc acacgcctgc
    12661 gtgaaggatt cgcggccctg tgaatccttc acgcaagggt gcgcggacaa tcccaccccc
    12721 aggccgcccg agggccccgg cgcgcgccac accgagcccc cgccgggatg cccgcggccc
    12781 ggcacgccct tcacccccct gcgacgacgc cccacccgcg ccgcgatccg aggccctcac
    12841 cggccgtgga gagccgcccc aacagccgca tggcctgccg gaccggcccg gatgtgacca
    12901 aattcacccc aaacgagtga atccgttcat atcccaaaag gatgtgccga cgtaccctca
    12961 gcttcgcagc acattcattc atcatcccac cgaaaggaga cagcatggcc acctcttccc
    13021 acacccgcgt cgcacccacc cagctcgaca ccgacgtgga cacctactcc gccctccggg
    13081 gactcgtcgg ctacacgccg cgcaacgccc gcttcagcac cgaatccgcc ggcgccgccc
    13141 tggccgccat gcgcgccgcc gacgaagccg ccatccaggc caacaacgcc ctcaccgtgg
    13201 cccgcgaaag cctcatcgcc gcccagaagg acttccacga gatcatcctg ggcgtgaagg
    13261 ccgaggccaa ggtgatcttt ggcgaagact cggacgaagt ggccgcgctg ggcctcaaac
    13321 gcaagtccga ccgggccggc cccaagcgca gcagcaagcc cacgcccccg accaaggaga
    13381 gttaatgggg ggcattagcc ctgcatcgag gcaacggcgg caaatctgcc gccgttgtgt
    13441 aagaataatg aatatgtgac ttctggatac gcgcgccgct ctcgtgcatc acacccgtcc
    13501 tgttcttcag cacaactacc ggcgtcagct aatgatcgat ctcgaaatgc agttgcgaag
    13561 ggctgatcgt ctagatgcca tcactaagag tctaggcttt gccttgtggc aacttcaagc
    13621 acttgaagaa acaactgcta actactacgt tctggtagca ctagctacac gtggtatggg
    13681 cattgaggca ggaaggaagc ttgatgactc agtaaagggc aatactttcg gaaaaacaat
    13741 tcacgcatta cgtgattctg gaaaaatgcc ctctaagata gaacagcgtt ttctagcctt
    13801 gcttaaggag cgcaattggt tagtgcacaa atctcgtgca accaatcgcg gtgccgtgca
    13861 cgacaatcaa gcttgcgatt cacttctcgt ccgattggat caacttgcag aagaagcgag
    13921 actgttgctt cgagagattg gcaaggcttc tgaaaaattt gttgtccaga gtggtgtgcc
    13981 aatagaagag gttgatcgtc taacgcacga aattctgaat tcatggcact ccactacgaa
    14041 tgagtaagcg cgaaatggag gttccagaaa tccctaggcc tcactgcgcg aaaacgatct
    14101 aattgtcttt gcggcgagat tgagttacag cggcttgcgg ggactcagtc cccacgagcg
    14161 gtcaataagc cccgtattca aaaactattg ccaaattctt gaggcggttt aaattttgag
    14221 ttttcaataa atctcaaatg gctgaaacag atcgagacct tggggttgct gccgcagcga
    14281 tcgaggcaat ggcaagtaca aaaaaactca gtgaattcaa atcattctgg gagtcgtttc
    14341 tctttcgcct tgagcgtgca tgggaacgca accaacggcg cgtgcagacc gtgccgggtc
    14401 gcgacgctaa gtcatggcta tccacaaatg ccaagctgcg ccgaagcgat cccttgttaa
    14461 attatctcaa acaggccaga aacgctgagg ttcatgcaat ttcatctagc attgagtccg
    14521 aattgatagt ttcggttgca gatcgatttg ggcggccatt tagcatgaaa gacatgagtc
    14581 tatcgattga gggtgcaact tttgtcatcg acctgaccag cactgacata ggcttagagt
    14641 ggtatggtga tgtcgcgctt tcagagccaa agcttcagtg cgtcataaat cgtggaaagc
    14701 ggtatgaccc accgactcgg catcttggta atgcaataca gaatcatcat ccggttgcga
    14761 tttcaattct tgggctcaat ttttacaaag gaacctatgc tgcgctcggg gcgcttctaa
    14821 atcataaaaa gccgtagcca aacagactag gcaataatct ccgttcctga attcgtcgtc
    14881 tttcccgccg tatcatgttt gccgcaacat tcttccgtcg cctgaactcc catattccac
    14941 ttaattttcc ttggttcttg gagaattccg aagccttgat cccggtgcgt tctcctgcct
    15001 cttctacgcc ggcttggcac caatcatcga tcttcgccac cttgacagcg ctctcgacag
    15061 gcatgaactg tctagaaaat gagattaatt ggccggggtg attaccgccc cgccttcttc
    15121 ctcaccagca tcaccggcgc gcagcgcccg gggtcgtggt agatccccac gcagtccagg
    15181 cactggaagc agcggtcgta gcggatggcg ccggattttt cgatggcgtc gtaggtgcag
    15241 cggtggcggc aggtctggca gggttggccg cattcggcgc ggcgggggag ccagtcgagc
    15301 aggcgcaggc ggccgccgag ggtcatggcg gcgccgaggg ggcagaggta gcggcagaag
    15361 aacttgtagt agaggccgcc ggcgatgagg agggcgacgg cccaggcgac gtagggcccg
    15421 tcccggttga agccgacggt gatggcggtc ttgaagggtt cgatctcgac gccctgttcg
    15481 gcgaggcggg gggcgaaggc ggcggcgagg acgagggcgg cgagcacggc gtagcggctg
    15541 cgttcgagga cgaggccgag gcgggcgggc atgcgcagcg cgggcaggcg cagggcgcgg
    15601 gcgaggaggg cggcgaattc ctggagcgcg ccgaaggggc acagccagcc gcagaaggtg
    15661 ccgcggcccc agacgacgaa gctgacggcg gtgaaggcga tgaggaggag gctgacgggg
    15721 tcgtagagga agctcttgag gccctggccg gcggcgaggg atttgacggc gccggtgatc
    15781 tgcacgatgg agagctggcc ctgggcgtac cagccaagat agaccagggt gaaggcgagg
    15841 aagccgaggc ggaagacgcg caggcgcggc gcggagatgg acatccagcg gggccgggcg
    15901 aggacgacgg tgaggccggc gagggcgagg ccgatgacga tgagctcggc ggcgcgatcc
    15961 ttccaggcga cgagccattc gggcaggggc cgcggcgggc ggacgaacag gcgctcgggg
    16021 ggctggtaat ccagcgcgac ggtcttctgg aaggtctggg gcaggatgct gcccttgctg
    16081 cgattgaggg tgagttcgaa gcgcacgggg ctggcggggt cgacgccgga catgggcggg
    16141 acgcgcagca ccagggcggc gttgaggggc ggggcgccag cggggggctt gggagagagg
    16201 tccaggtcgc gcagttccac cggtccaccg gcctggctga aggcgaggcg cacgggcacg
    16261 gtgccgcggg tgaactggtc gtcgatgaag gcgtcgcgac cggcggtggc gacccaccag
    16321 gcgtgctggc cgggttcgag gcggcgcatc agatcagcgt ggccggcgtc gccgaggagg
    16381 gcgcggccga tggtgggggc gttgaggtag gcgacgtaga ggtcggtgaa ggtgtcgtcg
    16441 gggcgggcga gggcgtcggc gtcgaggccg gcggcgtcgg tgccggcgaa gagcttttcc
    16501 acgtcggcgt tgcgcaggtg caggcggccg atggcgccgt cgtccacgag ctgggcgaag
    16561 ctgcgggcct cgtagagatc cgcgcgggcc tcggcggtgg ggcctttgtc cacgggggcg
    16621 gcgaagccga ggcgggcgcg ggccacggcg agggcggcgg tgaggacggt ctggttggcg
    16681 atgcgcacgg aggcggtggc cttgctgacg ccgtcgagca cgacgcgccg ggcgccagca
    16741 ccgctgcggg tgttgccgta gaggctggag acggtgatct cctgcttgag gctcttgcct
    16801 tcgtactggc gcaggaagtc tttgaggggg ccttcaccga ggcctgagag gaagaccggc
    16861 tcgtgctggc gcaggagctc gacgccaagg aagttgccct tgcggtcgat gctgacgagg
    16921 aggttgtagg gcgtgccttc gaaaccgggg atgggggcca ggtcgatgga ttcgaaggcg
    16981 taggcgacgg ggccggcctc cggttcgagt tcggaagtga tgggccaggc ggggatgtcg
    17041 cgcagcttgt cgcccacgtg gaggggcggc tggaagcggc gggcaatgtc gtcgcgggtg
    17101 aggtcgccgg cgtcggccag ccggggcgcg aacagggcgc aggcggcgag gagaaggctc
    17161 aggacgcccg ccaggccgcg ccaccgggac gcgggcgtgg gggcggacgg gcggcggccg
    17221 gggatgccct ccgcgggaag gggatggcgg agggcgtggg acatggcggg gacgggtcag
    17281 tgggcttcga gggtggcctt gaggttgccg aggccgccgc tgtagatgct gtggatgacg
    17341 cgccgggcgc ccgcgtcgtc agcgccgtcg aggggctttt cggccttgcg gcggaagcgc
    17401 gaggaccatt cgacgcggct gcccgccccg tccggcagga cgcgcagggt ggagcggtag
    17461 tcgctcaccg gcaggggcga gtcgatgatg gtgtagctga ggcggcgggc ggcgtcgtcg
    17521 cgggcgacga ggcgttcgac gagcttggcg ccgtcctttg tggtgacggt gcgcacggcg
    17581 ccaggccggt tgttctcgcc ctggtcgatg gtggtgctgg ccacggcggg gtgccagccc
    17641 aggttgccga agtcgccggc cgcggcccac acctgggcgg gcggtgcggc gatgacgatg
    17701 ctctctttga cgtggagcac ggggcgggcg gccagggcgg gcgccgcaat gaggggggtg
    17761 aacagcgcgg cgaggctggc gagggcgatt cgggacagag gatgcatggg gcgttccttg
    17821 gtttggaggt cagacttcga tgagaccggc gcggacggcg gccatggcca gctcggccat
    17881 gttctgcacg ccgagcttct gcttgatgtg atagaggtgg gtgcccacgg tgttggggct
    17941 gaggtggaag gtggcggcga cttcgttcac cgagcgcccg cgggcgagct gcaggaagac
    18001 gtggaattcc ttgtcggtga gggcttcggc ggggtcggcg gatttgtcgc cgcgggcgag
    18061 ttccggcgcg atgccggggt cgatgtaggt ctggccggcg gcgatgcggg cgacggcggt
    18121 gagcagttcg cccggcgggc tgcgtttgca caggtaaccg cgggcgccga gcttgagggc
    18181 gcgccgggcg gtgacggcgt cggcgtgggc ggaggcgatg agtacggcct gggcagggct
    18241 gcgggcgaga atgcgttcca gcgcggcgag gcctccgcag ccgggcatgg agagatccat
    18301 cacgagcacg tcggggcgca gctcggcagc caggcgctgg gcctcctcgc cgctctcggc
    18361 ctcgccgacg atgtgcacgc cgacgccttc gaggagcagg cggaagccca tgcggacgat
    18421 ggcgtgatcg tcggcgagga ggacggtgcg ggcgctgagt tcaggcgtca tggggggcgg
    18481 agtcggggag gggcaggacg gcgcggatgc gggtgccgcc gccgggtggg ctgctgatgt
    18541 gcagctcgcc ccgcaccgcg gcgacccgtt cgcgcatgcc ggtgaggccg tagccggact
    18601 ggccggacgc gggctgcagg ccgcggccat tgtcgcccac ctccaccacc agccggccgg
    18661 cctgacgcag cacgcggacg taggcctggc tggcctgggc gtggcggatg atgttggtgc
    18721 aggattcctg cacgatgcgc agggcgacga gggtaagttc gtcgcccagg ggcgccgggc
    18781 cggcgaagag ttcggtgacg aggcggatgt ccgggtggcg ttgctgccag gcgccgagca
    18841 cgctgcgcag ggcgtcgtcg agggtttctt cggcggggcg ctgggggcgc aggcgggtga
    18901 gcagggcgcg cacgtcgtcc tgcatgtggg cggccacgtc gcggatggtg cgggcgctct
    18961 gctgcacggt gggctggccg tcgctgcgtt gcaggatggc gccggccagg gcggcgacgg
    19021 cggtgatgct ctggcccagc tcgtcgtgga gctcgcgggc gatgacggtg cgctcggctt
    19081 cgaggcgctg gtgcaggacg tggccgaggg cctgctcgtg gtgcaggcgg gcgttgtcgg
    19141 caacggcctg gtcgaggcgc agggtcatgc ggttgaaggc ttcggcgagg cgggcgagct
    19201 cgcgcgggcc gtcttccggc aggcgcgtgt cgaagcgccc gtcgccggcg tggccgaggg
    19261 cggccatgac ctggccgagg gggcgcaggg cccggtccac cgccagccag cagccggcaa
    19321 acagggcgat gagggcgagc agggcgcggc cggcggcggc ggtgaggtcg tcccacagat
    19381 ccagcacggc gcgggaggcg tcgggctgga tgacgatgtc caggtggccg gcgggcaggc
    19441 tgcgggccgg gagatccggg ctcatccacg cgacgaacag gggcggggcg tcgcggccgg
    19501 ccttgtaggg cgaaccgggc gagcggtaga gcagggcgcc gcgggcgtcg cgcagttcga
    19561 cctggttggc gcgcaggcgc ccggcggcct gcaggtaggc gacgaggcgc ggctcgctcc
    19621 aggcggggtc gttgtcgctg gcggcgcggg ccgagacagt gagccactgc acggcgaccc
    19681 ggtgggcgga ggtgacttcc tcggcgatgg cgttgcgggc ttcggcgatc cacagggcgc
    19741 cgccggccag gatgaggagg gcggcgaagg cagtgaggat gaggctgatg cgcaggcgca
    19801 ggctgagccg gcgcgggcgg ggccagcgca gggcgcgggt ggtggcgtcg ggtgtcgggg
    19861 cggtccgggc gccgggggct gcggtcgggc tggcggtgtt catgattgct tgtcgagccg
    19921 gtactgcacc ggcacgacga tctggaactc gcgcccctgc agggcatccg gcacgcgggg
    19981 caggggggcg gcggcggtga cgagggcgat ggcgttctgg tccagcacct cgaagccact
    20041 ggagcgcagc acctgggtgg cgacgatggt gcctttgcgg gcgatggtga cgcgcagttg
    20101 cacttcgccc tcccagccgc gcagggccgc caggcgcggg tagttctgct ggcgggcaag
    20161 gaggccggaa aggctgcggc cgtagcgttc gaggagggcc ggatcggggg cttcgtccac
    20221 gtggggcgcc ggagcggctt cggccggcgt cgtgacggcg acgaccggcg caggacgtgg
    20281 gctggcttcg gccggcgcgc tgatgggggc gctggacggc actggcggcg tgggcgcctg
    20341 gacgatgacc ggagtgtcgg gcgcggcggc gcgggtcggg
//
LOCUS       DAOQPA010000060        20119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1320_length_20119_cov_19.157197, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000060 DAOQPA010000000
VERSION     DAOQPA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20119
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_20119_cov_19.157197"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..469)
                     /locus_tag="PKX01_09780"
     CDS             complement(251..469)
                     /locus_tag="PKX01_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HMZ76458.1"
                     /translation="MAIKDIKAFSDKARIEPELKDKLAACQKIRELLTLAKDSGFNLD
                     EQELYPPNEPQFTADQLSERLVKALLRA"
     gene            705..1955
                     /locus_tag="PKX01_09785"
     CDS             705..1955
                     /locus_tag="PKX01_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76459.1"
                     /translation="MPFLTDSPPGSADALRRLVGLRWLSVGAMAALLGAVPPLLDIAL
                     PVSALWPVVLGLAAVNGASVLRLRQGGEVTPVELFAQLCMDLAAWSALLYFTGGATNP
                     LISLLLPLVAIGAAILPAPYAWGLAVLAVAAYSLLWEFNEQLDIADSGLAVHWHLAGM
                     WLTFAVSAGVIVGYVVRMTALIRHRDQALAEAREERLRNERIVALGNLAAGAAHELGT
                     PLGTMAILAGELARNPRLDADTRADAALLGEQVAHCKAILGGLTLRAGSLRAEGGETQ
                     AADRWLDGIVERWRRLRPHVPLATHIDCGHPAPRLVADATLEQALHNLINNAADASPD
                     GVECEAALAGEALVVRILDRGPGLPEGCQPGQPTPATGRGLGIGLLLAFGAVERCGGR
                     IEFNPRPGGGTLATLTLPLSALCP"
     gene            1946..2488
                     /locus_tag="PKX01_09790"
     CDS             1946..2488
                     /locus_tag="PKX01_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76460.1"
                     /translation="MPMNDAPCLLVIDDDPAFNRVLSRALVQRGFEAYGAQDPESALA
                     LAREHQPEYVVLDLNLDGSSGLRLIEPLRAVNPDCRILVLTGYASIATTVDAIKLGAT
                     QYLAKPADVDAILKALNATDVLIDDDLAAGPMSVDRLEWEHIQRVLGEHEGNISATAR
                     ALKMHRRTLQRKLAKRPVKE"
     gene            2485..2883
                     /locus_tag="PKX01_09795"
     CDS             2485..2883
                     /locus_tag="PKX01_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMZ76461.1"
                     /translation="MKADEHPAERVRLDKWLWAARFFKHRSLATEAVDGGKIQLNGLR
                     VKPAKDVKPGDRVDIQIDATRFSVIVTGVAEKRGSAAVAQTLYTETAESIAARERQRE
                     ERRFAATPGDDLHGRPTKRDRRRIGRLQEG"
     gene            complement(2890..3762)
                     /locus_tag="PKX01_09800"
     CDS             complement(2890..3762)
                     /locus_tag="PKX01_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMZ76462.1"
                     /translation="MLLRIVSILFPLFALTALGYLVGRRQRPDLSHANKLNMDVFVPA
                     LVFAALANKSFHVADFVPLLGATLFLVGGCGLAGWALARGLGVAPRTLVPPLMFNNCG
                     NLGLPLAVLAFGDQALAPAVAMFMVSNLLHFSFGAWLLDHSISVAGVWKVPSVLATLA
                     GIAVGLLGIEVWAPAMTAIRMLGDISIPLMLFALGVRLTESRIGELRLGLIGAAARPL
                     IGMALALLAMQLIPLSKSEQALLLVFGALPPAVLNYIFAERYHQEPEKVASIVLLGNV
                     AALLFLPVALAIGL"
     gene            complement(3897..5234)
                     /gene="hslU"
                     /locus_tag="PKX01_09805"
     CDS             complement(3897..5234)
                     /gene="hslU"
                     /locus_tag="PKX01_09805"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HMZ76463.1"
                     /translation="MTQMTPPEIVSELDKHIVGQSRAKRAVAVALRNRWRRSQVAEPL
                     KSEITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDTIIRD
                     LVEIAIKSHREEAMKKVRDRAQDAAEDRILDVLLPPARPTMGFGNEAPSAEENATRQK
                     FRKKLREGDLDDKEVDIEVSAPGMTAEILAPPGMEELTGQLQSMFQNLGGGKKKSRKL
                     KIREALKLLTDEEAGRLVNDEDVKLEALRRVEQNGIVFLDEIDKIATRSDSHGADVSR
                     QGVQRDLLPLVEGTTISTKYGMVKTDHILFIASGAFHLSRPSDLIPELQGRFPIRVEL
                     ESLSVEDFERILTQTDACLTRQYEALLATDGVSLSFAPDGIRRLAEIAFQVNEKTENI
                     GARRLYTVMEKLLEELAFEAGRAGNQTVNIDAAFVDGRLETLAQREDLARYVL"
     gene            complement(5245..5784)
                     /gene="hslV"
                     /locus_tag="PKX01_09810"
     CDS             complement(5245..5784)
                     /gene="hslV"
                     /locus_tag="PKX01_09810"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HMZ76464.1"
                     /translation="MEQYHGTTILSVRRGRHVALGGDGQVTLGNIVIKASARKVRRLY
                     QDRVLIGFAGGTADAFTLFERFEAKLEKHQGHLLRSAVELAKDWRTDRALRRLEAMLA
                     VADLDNSLIITGNGDVLEPEQGIVAIGSGGAYAQAAARALVENTELAPEDVVKKALSI
                     AGDLCIYTNQSHTIEVLKP"
     gene            complement(5868..7064)
                     /locus_tag="PKX01_09815"
     CDS             complement(5868..7064)
                     /locus_tag="PKX01_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ76465.1"
                     /translation="MVLPFFGKKPPPAGSSTPRSSARSPSGSSAAAARPGLPQPDRNE
                     TSSLDFTIAGGDIKRVLAQCADRVHVEEGVDEMSPPAEEAAILYANGAWNEARSVLEH
                     ALQTTGSSDARLGVWVMLLDLLRLTGQREQFDALSLAFATEFERSPPAWQDLSSQSAR
                     PMASKTPLVNLSGALSGQAAAQFAQLIQVGMRSGALRIDLTRLRSIDQAGAELLLRAV
                     RGLRKARIRLSLVGAPHLVNLVSGQVVRGRADNKTQWLLLLEALQYTDQQERFEELAV
                     DYAMTFEESPPSWEPPMVTESQTEAVEAVGMNDIFVLEGEVVGPNAEALRKLAAFAND
                     RNKIEVDCGRLRRMDFVSAGNLFNIVSQLHSKGRLVVLKSVNSMVAALMQVMSLHEVA
                     RIELRG"
     gene            complement(7164..7955)
                     /locus_tag="PKX01_09820"
     CDS             complement(7164..7955)
                     /locus_tag="PKX01_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HMZ76466.1"
                     /translation="MSLEFLVDPLFRLPFLTGLALAGLLPVLGMYLRLRNEWLAALAF
                     AQMASGGALVAMVADAPMLLGGAVAALLAALVKGFAARAGASAYALMMLLGWGGGVLL
                     VANHPLAEQAGHALFDGQLYFTGGAHLLAALIAVIVAMPVLAWLSRPLLLGRFFPEFF
                     RARGLDERPRLVLFDLIAGLSVALATVSLGVMAAFALVFVPPMVVFPRAPGWRVGLLV
                     AAVVGMGGYVTAFALALVFDQPFGPVCAILLVLLSGLVGLIGRQS"
     gene            complement(7952..8533)
                     /locus_tag="PKX01_09825"
     CDS             complement(7952..8533)
                     /locus_tag="PKX01_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ76467.1"
                     /translation="MSGHLLRVSGLVAGYREPVTAPVSFTLKRGEILGIAGPNGAGKS
                     TLLKALWGGVRVFAGAVEKAPGLSISHQAQGFDDLRGVPLSGRELLRLTGAPPDGLPP
                     WVQERLDRRLDRLSGGQFQFLRLWACLAAPADLILLDEPSNNLDTAGVAYLADWLGRA
                     HGDQGIVIVSHDAALMAQVCTRILHVDAVEVAA"
     gene            complement(8544..9452)
                     /locus_tag="PKX01_09830"
     CDS             complement(8544..9452)
                     /locus_tag="PKX01_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HMZ76468.1"
                     /translation="MLRTLLLRLVALVLFTSAGAASAALNVFATVPEWAALAKEIGGD
                     QATVFAATTALQDPHRIEARPSLIARARSAGLVVATGAELEIGWLPMVLRESGNAAVQ
                     PGQPGYFEAATVVRMLEVPTRLDRADGDVHPAGNPHLQLDPRNILRVGEALAARLAQV
                     DPAHAEVYKANLARFGERWRAAQARWEQAAAPLRGVPVWVQHRAFPYLSAWLGLKEVG
                     ALEAKPGVEPGSAQLADVLARQKAMPARMVLRPAYARATASDWLAERAGIPAVVLPFT
                     VGGSAEAQDLFGLFDDTVRRLLAALK"
     gene            complement(9464..10882)
                     /locus_tag="PKX01_09835"
     CDS             complement(9464..10882)
                     /locus_tag="PKX01_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76469.1"
                     /translation="MSIVRPGALCVALAAALPAVASAADSAADLKQLRDELRALRDTY
                     QQRIDALEKRLADSEARAARAETTATAAARSAEQAEQKAAALPDVAIAPATPPTGEGA
                     FNPAVSLILDSKFTRFQQDPAKYRLDGFIPSGGEVGPPRKGFSLGESELAVSANVDHL
                     FRGNARFSLADDGAGGTVEVEEAHIETLALPEGLKLKAGRFLSGVGYLNVQHPHEWDF
                     TDAPLAYKAFLGPRLQNDGVQLRWVAPTDLFVELGGELASGQKYPGAERAANGAGLWT
                     AFAHVGGDVGASSAWQVGLSHVHANPRARTFEDGGVSNAFSGRSRLWIADFVYKWAPD
                     GNPTVNNLKLQGEFFRRSEDGDLTYDLAGAGQVSAASFRQSGGYLQAVYQFMPHWRAG
                     VRGDWLRAGSIDLGSLSTTELPGLVVYNPSRQSAMVDWSPSEFSRLRLQLARDTSRAG
                     ETDHQLWLQYIMSLGAHGAHKF"
     gene            complement(10963..11328)
                     /locus_tag="PKX01_09840"
     CDS             complement(10963..11328)
                     /locus_tag="PKX01_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76470.1"
                     /translation="MRRMPFRGRPSALFRGTLALLLVCAQLLGAMHAIGHFEAAERPL
                     RVDAQAAPASGGQPATERHERCLLCLAAADLGAALPVTPSLPGPEAPVSALPPDAGTL
                     PAFLRPPTPSSRDPPVSPV"
     gene            complement(11325..12392)
                     /locus_tag="PKX01_09845"
     CDS             complement(11325..12392)
                     /locus_tag="PKX01_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ76471.1"
                     /translation="MTDPMRAAHRIPVTLLTGFLGAGKTTLLNHLLRRPDMAGTVVVI
                     NEFGSIPLDHLLVTHLDEQVVLLESGCLCCTVRGDLSRCLRELFMRRLRRELPALDRV
                     IVETTGLADPAPVLFTLMNDFFVAERYRLDGVVTAVDGVFGADQLAARPEAVKQAAMA
                     DRLVITKVDQATPAQLDAVDARLARLNPGAPRLRSVGGAVDPADLLDCGLWNPVTHSA
                     DVARWLGDGAVAAQRDPDYRPLTAKPSAPAGQHDAAVRAFALRLPAPVAWGDFSAALD
                     ALQSLWGERLLRVKGLVHVAGEVSPRVIQCVHHLRYPDAALPAWPQGARETRLVFIVR
                     ELDPAVVERAFACFCVPELVA"
     gene            complement(12435..12863)
                     /gene="dksA"
                     /locus_tag="PKX01_09850"
     CDS             complement(12435..12863)
                     /gene="dksA"
                     /locus_tag="PKX01_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HMZ76472.1"
                     /translation="MVATTKDKILTEAEILAAPEEEYMNPAQLEFFRDRLSRMRQELL
                     DNAASTGASLRENEIVADPADRATVEEEHALELRVRDRERKLLKKIDEALNRIAEGEY
                     GWCEETGDPIGIGRLLARPTATLSIDAQERRERTQKLYGE"
     gene            13154..13609
                     /gene="ybgC"
                     /locus_tag="PKX01_09855"
     CDS             13154..13609
                     /gene="ybgC"
                     /locus_tag="PKX01_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HMZ76473.1"
                     /translation="MHAQTDPARPALSEQSVRPSFTLPVRVYYEDTDAGGVVYYANYL
                     KFCERARTDWLRSCGIGQHRLASEDGVLFVVRSVRADYRQPAVLDDDLAVVSTLAKLG
                     HASLTFHQKVLRDGVELFDAQVVIACIDRDARRAIPIPPAVRAQFATLV"
     gene            13614..14288
                     /gene="tolQ"
                     /locus_tag="PKX01_09860"
     CDS             13614..14288
                     /gene="tolQ"
                     /locus_tag="PKX01_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HMZ76474.1"
                     /translation="MNVTEDLSILSLIANASLLVKLVMALLGTVSFMSWYWIFRKWFQ
                     IRDAKKKTDLFERDFWSGGDLNALYQSAATSRHEAAGMERIFESGYREFAKLRGKNLD
                     HGAIMDGAKRAMRATYQREVDDLEAHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLSN
                     VGSATLAHVAPGIAEALVATAIGLFAAIPAVVAYNRFAHDIDRIAIRFESFTEEFLNI
                     LQRQLR"
     gene            14305..14718
                     /gene="tolR"
                     /locus_tag="PKX01_09865"
     CDS             14305..14718
                     /gene="tolR"
                     /locus_tag="PKX01_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HMZ76475.1"
                     /translation="MRQRRLMNQINVVPYIDVMLVLLVIFMVTAPMIQSGSVNLPSVD
                     KVPAPPSDAMIVTVKPDGELTLAQTSQSQPRSYNRRDLIGALQDATSANPGQPVVIAA
                     DKDVKYERVMDILNDLRNAGVNKVGLQTQIGKSAR"
     gene            14715..15680
                     /gene="tolA"
                     /locus_tag="PKX01_09870"
     CDS             14715..15680
                     /gene="tolA"
                     /locus_tag="PKX01_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HMZ76476.1"
                     /translation="MNPALPGNERGKWSSAALAAGVHVLLGLFLFYGVRWQNRLPEPV
                     EVELVSAPPVTAPVTPPPPPPAPAEERPAPPPPPRPEPEPEPAPPPRKQPDIAIKAPE
                     PRKLPPKPVKEVVKPEPKPEPKKPEPKPEPRKPEPKPEPKKPDPKPEPKPEPKPDPKA
                     EAKARAEQARLDKAKADKDAKAASDRAREQEDRRMRELLAHDSENAKQSAQIQEELAR
                     IAGAKASAARNKAIEAYIEKIRAKVKGNIVLPPGVAGDPFALFYVDQLPDGSVIEVRL
                     KRSTGTPQLDSAIERAIQKSSPLPRPDKPELFNRTLELKFYPLRD"
     gene            15739..17028
                     /gene="tolB"
                     /locus_tag="PKX01_09875"
     CDS             15739..17028
                     /gene="tolB"
                     /locus_tag="PKX01_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HMZ76477.1"
                     /translation="MKATFLTLLRGLLPGCALALAAATAQAQLAIEITGAGANRLPVA
                     IAAFEGEAALPRPVTDVVKADLERSGLFKLIELGPTPQGYDAQVNLPGVRSRGADALA
                     VGSVVTTGNGRYEVRVRLYDTPKQAALGVQAMTMSPAQYRTTGHRIADFIYEKLTGEP
                     GVFSTRIAYVLKSGPRYELQVADADGSNAQTALASKEPIISPAWSPDGTRLAYVSFES
                     KKPVVYVHSLATGQRNVAANFKGSNSAPAWSPDGRRLAVVLTKDGQSQLYAVNADGSG
                     VQRLASSAGIDTEPAWSPDGAWIYFTSDRGGSPQIYRIPSSGGDAQRVTFEGSYNVSP
                     RPAPDGSNLVYVSRNGGRFQVTMMDLNSRQTQILTDSSKDESPSFAPNGRMILYATEI
                     GGRGVLAAVSSDGRVKQRLSVQSGDVREPAWGPQPRN"
     gene            17103..17657
                     /gene="pal"
                     /locus_tag="PKX01_09880"
     CDS             17103..17657
                     /gene="pal"
                     /locus_tag="PKX01_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HMZ76478.1"
                     /translation="MNIPRFALTLAAAGILAACSSSPKTGDEQSGAAVEDRSGAASSG
                     IDSSRVVPVTPGDAAGAGIAALKDPKSILSKRSVYFDYDSYVVKDEFRALVEAHAKFL
                     VGHPQMKMLIQGNTDERGSREYNLALGQKRSEAVKKALSLLGVPESQLESVSLGKEKP
                     SCSDATETCWSANRRGDMLYSGEF"
     gene            17657..18421
                     /gene="ybgF"
                     /locus_tag="PKX01_09885"
     CDS             17657..18421
                     /gene="ybgF"
                     /locus_tag="PKX01_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HMZ76479.1"
                     /translation="MKQRLLPLLLAGLVVGLAPLRAHAGLFDDDEARARIEDMRKQLS
                     ARIDKLEASARAQLELANQIEQLKAEIARLRGENEVLANDLANANKRQKDFYVDLDNR
                     LRKVEPPVAAAADPAATPVQPAPQPPAVDPANENRDYEAALNQLRGGKYKEAATAFTA
                     FLRNYPNSSFQPSAHYWTASAQYQLKDYANAADHYGRVASQWPDDTRAPDALLGLSSA
                     QSGLGDLKAATRSLEKLVAKYPNSQAAQIARQRLKK"
     gene            18474..19136
                     /gene="queE"
                     /locus_tag="PKX01_09890"
     CDS             18474..19136
                     /gene="queE"
                     /locus_tag="PKX01_09890"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMZ76480.1"
                     /translation="MNTPSGHPVTLRITEIFHSLQGETSRAGLPTVFVRLTGCPLRCT
                     WCDTTYSFTGGTRRSLDDVLAEIAAYACPTVCVTGGEPLAQKGCLALLTALCDAGYSV
                     SLETSGALDIADVDPRVSRIVDLKAPGSGEEARNLLSNLDHLAPQDELKLVLASEADY
                     LWARHMLAEHRLAERCPVLLAPVQGQLPPADLAAWILRDRLPVRMQVQLHKVIWGNEP
                     GR"
     gene            19195..19878
                     /gene="queC"
                     /locus_tag="PKX01_09895"
     CDS             19195..19878
                     /gene="queC"
                     /locus_tag="PKX01_09895"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HMZ76481.1"
                     /translation="MNTPRPRAIVLLSGGLDSTTCLAIARAEGFDAYALSVEYGQRHA
                     AELAAARRIAAALGAIDHRVAHVDLGQFGGSALTDPALAVPVDGVAGGIPITYVPARN
                     TIMLSIALAWAEVLGADDIYVGVNAVDYSGYPDCRPAYIDAFEKMANLATKAGVEGHR
                     LRIHAPLIDLSKADIVRRGAALGVDYGLTVSCYQAADDGAACGLCDACRLRSAGFAAA
                     GLPDPTRYR"
     gene            19945..20020
                     /locus_tag="PKX01_09900"
     tRNA            19945..20020
                     /locus_tag="PKX01_09900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19978..19980,aa:Lys,seq:ttt)
BASE COUNT         3309 a         7308 c         6630 g         2872 t
ORIGIN      
        1 gccgcagccg gccgggtgtg caaacccggc cgtggatcga aaccccggcc gacgactggc
       61 gaaaccagct ctaccagctc caaaagcgac gcaccgcaaa gccgaggccc accaggcagg
      121 gcgggtagcg caggtttgac gtaagtttgg ccgggtctcg gcccggcagc cgacaggcga
      181 aacccgcact accgcctcca gaggcgacac gctgtcaatc accgtgccgc cgcttgcggc
      241 aaaaagccga tcaggcccgc agcagcgcct tcaccagccg ctccgacaac tgatccgccg
      301 tgaattgcgg ttcattgggc ggatagagct cctgctcgtc caggttgaag ccggagtcct
      361 tcgccagggt gagcagttcg cggatcttct ggcaggcggc cagcttgtcc ttgagttccg
      421 gttcgatgcg ggccttgtcg gagaaggcct tgatgtcctt gatagccatg gtggttcctg
      481 tacggtttga acggatcgtg actgccggaa acgcgacaag acccggcggc tttgtcgcaa
      541 agctgccgca gaagtgcaca tttccggctg tcggcccctt ccttagcaaa gaccgcgccg
      601 cctgcgacct tccgccacat tccgggggcc gggcgggagc gttaaagtgg ctggccgtgc
      661 ttcgacccgc catgccgccg ccccgcttcg gggataatcg cccgatgccc ttcctgaccg
      721 actctccgcc cggctccgcc gatgccctgc gccgcctcgt gggtctgcgc tggctgtccg
      781 tgggggccat ggcggcgctg ctcggggcgg tgccgccgct gctggacatc gccctgccgg
      841 tgtcggcctt gtggccggtc gtgctggggc tggcggcggt caatggggcc agcgtgctgc
      901 gcctgcgcca gggcggcgag gtgacgccgg tggagctgtt cgcccagttg tgcatggatc
      961 tggcggcctg gagcgccctg ctctacttca ccggcggcgc caccaatccg ctgatctccc
     1021 tgctgctgcc cctggtggcc atcggcgcgg cgatcctgcc ggcgccctac gcctggggcc
     1081 tggcggtgct ggcggtggcg gcctattccc tgctgtggga gttcaacgag cagctggaca
     1141 tcgccgattc gggtctggcg gtgcactggc atctggcggg catgtggctg accttcgcgg
     1201 tgtcggcggg ggtcatcgtg ggctacgtgg tgcgcatgac ggcgctgatc cgccaccgcg
     1261 accaggccct ggccgaggcc cgcgaggagc gcctgcgcaa cgagcgcatc gtggccctgg
     1321 gcaatctggc ggcgggcgcg gcccacgagc tgggcacgcc cctgggcacc atggccatcc
     1381 tggccggcga gctggcccgc aatccgcgcc tggacgccga cacgcgggcc gacgcagcgc
     1441 tgctggggga acaggtggcc cactgcaagg cgatcctggg cgggctgacc ctgcgcgccg
     1501 gcagcctgcg cgccgagggc ggcgagaccc aggccgccga ccgctggctc gacggcatcg
     1561 tggaacgctg gcgccgtctg cgcccccacg tgcccctggc cacgcacatc gactgcggcc
     1621 acccggcgcc ccgcctggtg gccgacgcga ccctggagca ggccctccat aacctcatta
     1681 acaacgccgc cgacgccagc ccggacggcg tcgaatgcga agcggccctc gccggcgagg
     1741 ccctcgtggt gcgaattctc gaccgcggcc cgggcctgcc ggaaggttgc caaccgggcc
     1801 aaccgacgcc ggccaccggc cgcggcctgg gcatcggcct gctcctcgcc ttcggcgccg
     1861 tggaacgctg cggcgggcgc atcgagttca acccgcgccc cggcggcggc accctcgcca
     1921 ccctgaccct gcccctgtcg gccctctgcc catgaacgac gcgccctgcc tcctcgtcat
     1981 cgacgacgac cccgccttca accgggtcct cagccgggcc ctcgtccagc gcggcttcga
     2041 ggcctacggc gcccaggacc cggagagcgc cctggccctg gcccgggaac atcagccgga
     2101 atacgtggtg ctggacctga acctggacgg cagctccggg ctgcgcctga tcgagccgct
     2161 gcgggcggtc aatccggact gtcgcatcct ggtcctcacc ggctacgcca gcatcgccac
     2221 cacggtggat gcgatcaagc tgggcgccac ccagtatctg gccaagccgg ccgacgtgga
     2281 cgccatcctc aaggccctca acgccaccga cgtgctcatc gacgacgacc tggccgccgg
     2341 gcccatgtcg gtggatcggc tggagtggga gcacatccag cgcgtgctcg gcgaacacga
     2401 gggcaacatc tcggccaccg cgcgggcgct taaaatgcac cgacgtaccc tgcaacggaa
     2461 actggccaag cggccggtca aggaatgaaa gccgacgaac accccgctga gcgcgtccgc
     2521 ctcgacaaat ggctgtgggc ggcccgcttc ttcaagcacc gcagcctggc caccgaggcc
     2581 gtggacgggg gcaagatcca gctcaacggc ctgcgcgtaa agcccgccaa ggacgtgaag
     2641 cccggcgacc gcgtcgacat ccagatcgac gcgacccgct tcagcgtgat cgtgacgggc
     2701 gtggcggaga agcgtggcag cgccgcggtg gcccagaccc tctacaccga gaccgccgag
     2761 agcatcgccg cgcgggaacg ccagcgcgag gagcggcgct ttgccgccac gccgggggac
     2821 gacctgcacg gccgccccac caagcgcgac cgccgccgca tcggccgcct gcaggaaggc
     2881 tgaggccggc tacagaccga tggccagggc caccggcagg aacagcaggg ccgccacgtt
     2941 gcccagcagc acgatggagg ccaccttctc cggctcctgg tggtagcgct cggcgaagat
     3001 gtagttgagc accgccggtg gcagggcgcc gaacaccagc aggagcgcct gttcggactt
     3061 tgacagggga atcagctgca tcgccagcag cgcgagggcc atgccgatga ggggccgcgc
     3121 cgcggcgccg atcagcccga ggcgcagctc gccgatgcgc gactcggtga ggcgcacacc
     3181 cagggcgaag agcatcagcg ggatggagat gtcccccagc atgcggatcg ccgtcatggc
     3241 cggcgcccac acctcgatac ccagcagccc gaccgcgatg cccgccaggg tggccagcac
     3301 ggagggcacc ttccacaccc cggcgacgct gatggagtgg tccagcagcc aggcgccgaa
     3361 ggagaagtgc agcaggttgg agaccatgaa catggccacc gccggcgcca gggcctgatc
     3421 gccgaaggcc agcaccgcca ggggcaggcc caggttgccg cagttgttga acatgagggg
     3481 cggcaccagg gtgcgcggcg ccacccccaa cccccgcgcc agggcccagc cggccagccc
     3541 gcaaccgccc accaggaaca gggtggcgcc cagcaggggc acgaaatccg ccacgtggaa
     3601 ggacttgttg gccagggcgg caaagaccag cgccggcacg aacacgtcca tgttcagctt
     3661 gttggcgtgg gagaggtcgg gccgctggcg ccggccgacc agatagccga gcgcggtgag
     3721 ggcgaagagg gggaagagga tggaaacgat gcgcagcagc atggccggaa ggggaatctc
     3781 cggtcggatg ccggcggacc gccattgtag ggccgacccg gccgccacgg ggcagccagg
     3841 atacgcacgc ggccccgcgc cccggcacgc cgcgaagggc gcgccggacg ccgcgcttac
     3901 agcacgtagc gggccaggtc ctcgcgctgg gcaagggtct cgaggcgtcc gtccacgaag
     3961 gcggcgtcga tgttgacggt ctggttgccg gcgcggccag cctcgaaggc gagctcttcc
     4021 agcagctttt ccatgacggt atagagccgg cgcgcaccga tgttctcggt cttctcgttc
     4081 acctggaagg cgatctcggc gaggcggcgg atgccgtccg gcgcgaagct caggctgacg
     4141 ccatcggtgg cgaggagggc ctcgtactgg cgcgtgaggc aggcgtcggt ctgggtgagg
     4201 atgcgctcga agtcctccac ggacagggac tccagctcca cgcggatggg gaagcggccc
     4261 tgcagctcgg ggatcaggtc gctcgggcgg gacaggtgga aggcgccgct ggcgatgaag
     4321 aggatgtggt cggtcttcac catgccgtac ttggtgctga tggtggtgcc ttccacgagg
     4381 ggcagcaggt cgcgctgcac gccctggcgg gagacgtccg cgccgtggct gtcggagcgg
     4441 gtggcgatct tgtcgatctc gtccaggaag acgatgccgt tctgttccac ccgacgcagg
     4501 gcttcgagct tcacgtcctc gtcgttcacg aggcggccgg cctcctcgtc ggtgaggagc
     4561 ttgagggctt cgcggatctt gagtttgcgg gacttcttct tcccgccgcc caggttctgg
     4621 aacatgctct ggagctggcc ggtgagctct tccatgcccg gcggggcgag gatctcggcg
     4681 gtcatgccgg gggcgctgac ctcgatgtcc acttccttgt cgtccaggtc gccctcccgc
     4741 agcttcttgc ggaatttctg gcgggtggcg ttctcctcgg cgctgggcgc ttcattgccg
     4801 aagcccatcg tgggccgggc cggcggcagc agcacgtcga ggatgcggtc ctcggcggcg
     4861 tcctgggcgc ggtcgcggac ctttttcatg gcctcttccc ggtgggactt gatggcgatc
     4921 tccaccaggt cgcggatgat ggtgtccaca tcgcgcccca cgtagcccac ctcggtgaac
     4981 ttggtggcct ccaccttgat gaagggggcg ttggcgaggc gcgccaggcg gcgggcgatc
     5041 tcggtcttgc cgacgccggt ggggccgatc atcaggatgt tcttgggggt gatctcgctc
     5101 ttcaggggct cggccacctg gctgcgccgc cagcggttgc gcagggccac ggcgacggcg
     5161 cgcttggcgc gggactggcc gacgatgtgc ttgtcgagtt cggagacgat ctccggcggg
     5221 gtcatctggg tcatggggtc gggatcaggg cttcagaacc tcgatggtgt ggctctggtt
     5281 ggtgtagatg cacaggtcgc cggcgatgct gagggccttc ttgaccacgt cttcgggcgc
     5341 cagctcggtg ttctcgacga gggcgcgggc cgccgcctgg gcgtaggcgc cgccgctgcc
     5401 gatggcaacg atgccctgct ccggctccag cacgtcgccg ttgccggtga tgatcaggga
     5461 gttgtccaga tcggccaccg ccagcatggc ctccaggcgg cgcagggcac ggtcggtacg
     5521 ccagtccttg gcgagctcga cggcgctgcg cagcaggtgg ccctggtgct tctcgagctt
     5581 ggcctcgaag cgctcgaaga gggtgaaggc gtcggcagtg ccgccggcga agccgatcag
     5641 gacccggtcc tggtagagcc gccgcacctt gcgcgcactg gccttgatga cgatgttgcc
     5701 gagggtgacc tgcccgtcgc cgccgagggc cacgtggcgg ccgcgccgga ccgagaggat
     5761 ggtggtgccg tgatattgtt ccatcgcgga aatatggggt cggtgagaag gaatgcaagc
     5821 ccccgcgaac gcgggcagcc aagggcgccc acccggtggg cgcgaattca gccgcgcagc
     5881 tcgatgcgcg cgacttcgtg caggctcatc acctgcatca gtgccgccac catgctattg
     5941 acgctcttga gcaccaccag gcggcccttg ctgtgcaact gggagacgat gttgaagagg
     6001 ttgcccgcgc tgacgaagtc catgcgccgc aggcgcccgc agtccacctc gatcttgttg
     6061 cggtcgttgg caaaggcggc cagcttgcgc agcgcttcgg cattggggcc gaccacttcg
     6121 ccttccagca cgaagatatc gttcatgccg accgcctcga ccgcctcggt ctggctctcc
     6181 gtgaccatcg gcggctccca ggacggtggc gattcctcga aggtcatggc gtaatccacc
     6241 gccagttcct cgaagcgctc ctgctggtcg gtgtattgca gcgcctcaag cagcaacagc
     6301 cactgggtct tgttgtcggc ccgtccgcgg accacctgcc cggagacgag attcaccaga
     6361 tggggggcgc ccaccagaga caggcggatg cgcgccttgc gcaggccacg caccgcacgc
     6421 aggagcagct cggccccggc ctggtcgatg ctgcgcaggc gcgtgaggtc gatgcgcagg
     6481 gcccccgagc gcatgccgac ctggatcagc tgggcgaact gcgctgccgc ctggccggac
     6541 agggcgccgg aaagattgac cagaggtgtc ttgctggcca tcggacgggc gctctggctg
     6601 gacagatcct gccaggccgg cggcgagcgt tcgaattcgg tggcgaaggc cagactcagg
     6661 gcatcgaact gctcgcgctg gccggtgagc cgcagcagat cgaggagcat cacccacacg
     6721 cccaggcgcg catcgctgga cccggtggtc tgcagggcat gctccagcac cgagcgggcc
     6781 tcgttccagg caccgttggc atacaggatg gcggcttctt ctgcaggcgg cgacatctcg
     6841 tcgacgcctt cctccacatg cacacggtcg gcacactggg ccagcacgcg cttgatgtcg
     6901 cccccggcga tggtgaagtc gagggaggac gtttcgtttc gatcgggctg gggcaggccc
     6961 ggacgcgccg ccgccgcgga tgatccggac ggactccgcg cactcgaacg gggcgtgctg
     7021 cttccggcag gcgggggctt cttgccgaaa aacggtagca ccacaacaac gtactccaga
     7081 tgaattcaac aggggatgaa cgattaggat aacaccgtca acaccatgac gaaccgtgaa
     7141 ttacttgcgc tcacaggtgt gaattatgac tgccgtccga tcaggccgac gaggcctgac
     7201 aatagcacca gcagaatcgc gcagaccggt ccgaagggct gatcgaacac cagcgccagg
     7261 gcgaaggccg tcacataacc gcccatcccc accacggccg ccaccagcag gccgacgcgc
     7321 cagccgggcg cccgcggaaa caccaccatc ggcggcacga acaccagcgc gaacgctgcc
     7381 atcactccca ggctcaccgt cgccagggcc acggacaatc ccgcgatcag gtcgaacagc
     7441 accagccgcg gccgctcgtc caggccacgg gcgcggaaga actcgggaaa gaagcgtccc
     7501 agcagcagcg gacgcgacag ccaggcgagc acgggcatcg ccacgatcac ggcaatcagg
     7561 gcggccaaca ggtgcgcccc cccggtgaag tacaactgcc cgtcgaagag ggcatggccg
     7621 gcctgctccg ccaggggatg gttggccacc aggagcacgc cgccgcccca cccgagaagc
     7681 atcatcaggg cataggcgct ggccccggcg cgggccgcga agcccttgac cagggccgcc
     7741 agcagcgccg ccacggcgcc gcccagcagc atgggcgcat cggccaccat ggccaccagc
     7801 gccccgcctg acgccatctg ggcgaaggcc agcgcggcca gccattcgtt gcgcaggcgc
     7861 aggtacatgc ccagcaccgg caacaggccc gccagggcga gtccggtgag gaagggcagg
     7921 cggaacaggg gatcgaccag aaattcgagg ctcatgccgc cacctccacc gcgtccacgt
     7981 gcaggatgcg cgtacacacc tgcgccatca gcgccgcatc gtgggagacg atcacgatgc
     8041 cctgatcgcc gtgagcccgc ccgagccagt ccgcaagata ggcgacgccg gccgtatcca
     8101 ggttgttgga cggttcgtcc agcaggatca ggtcggccgg cgcggccagg caggcccaca
     8161 ggcgcaggaa ctggaactgg ccgccggaca gccggtcgag gcgccggtcg aggcgctcct
     8221 gcacccacgg cggcaggccg tccggcggtg cgccggtcag gcgcagcagc tcgcgcccgg
     8281 acaggggcac cccacgcaga tcgtcaaagc cctgggcctg gtgggaaatg ctcaggccgg
     8341 gcgccttctc cacggcgccg gcaaacaccc gcacgccgcc ccacagggcc ttgagcaggg
     8401 tcgatttacc ggccccgttg ggaccggcga tgccgaggat ctcgccccgc ttcagcgtga
     8461 agctgaccgg agccgtcacc ggctcgcggt agccggccac cagaccgctc acccgcaaca
     8521 gatgcccgga catcgccgcc cccttacttc agcgcggcaa gcaggcgccg caccgtgtcg
     8581 tcgaagagcc cgaacagatc ctgcgcctcg gccgagccgc ccacggtgaa gggcagcacc
     8641 accgccggaa tgccggcccg ctccgccagc cagtcggacg cagtggcacg ggcataggcc
     8701 gggcgcagca ccatgcgcgc cggcatcgcc ttctggcggg cgagcacatc ggcaagctgg
     8761 gcgctgcccg gctccacgcc aggcttggcc tccagggcgc cgacttcctt caggccaagc
     8821 cacgcggaca ggtagggaaa ggcccggtgc tggacccaca ccggtacacc gcgcaggggc
     8881 gccgccgcct gctcccagcg cgcctgggcg gcccgccagc gctcgccgaa gcgggccaga
     8941 ttggccttgt agacctccgc gtgggccgga tccacctggg ccagccgcgc cgccagggcc
     9001 tcgcccaccc gcaggatgtt gcgcggatcg agctggaggt ggggattgcc ggcgggatgc
     9061 acgtccccgt cagcccggtc caggcgggtc ggcacttcca gcatgcgcac caccgtcgcc
     9121 gcttcgaagt agccgggctg gccgggctgc accgcggcgt tgccggactc ccgcaagacc
     9181 atgggcagcc agccgatctc cagctcggcg ccggtggcca ccaccagccc ggcgctgcgg
     9241 gcgcgggcga tcagcgaggg gcgtgcctcg atgcggtgcg ggtcctgcag ggccgtggtg
     9301 gcggcgaaga cggtcgcctg atcgccgccg atctccttgg ccagggcggc ccattcgggc
     9361 acggtggcga agacgttgag ggccgccgac gcagcgccgg cggacgtgaa cagcacgagc
     9421 gccacgaggc gcaacagcag ggtacggagc atggtcgtgt tcctcagaac ttgtgggcgc
     9481 cgtgggcgcc gaggctcatg atgtattgca gccagagctg gtgatcggtc tcaccggcgc
     9541 gggaggtgtc gcgggccagc tgcaggcgca ggcgcgagaa ctccgacggc gaccaatcca
     9601 ccatcgccga ctggcggctc gggttgtaga ccaccaggcc ggggagctcc gtcgtgctca
     9661 ggctccccag gtcgatgctg ccggcccgca gccagtcacc ccggaccccg gcgcgccagt
     9721 ggggcatgaa ctggtagacc gcctgaagat aaccgccgga ctggcggaaa cttgccgccg
     9781 atacctgccc ggcacccgcc aggtcatagg tgagatcccc gtcctcgctg cgccggaaga
     9841 attcaccctg cagcttgagg ttgttgaccg tcggattgcc atccggcgcc cacttgtaga
     9901 cgaagtcggc aatccacagc cggctgcggc ccgagaaggc gttgcttaca ccgccgtcct
     9961 cgaaggtccg cgcgcgggga ttggcatgga cgtgggacag cccgacctgc cacgccgacg
    10021 aggcacccac atcgccgccc acatgggcga aggccgtcca cagcccggcc ccgttggcgg
    10081 cccgctcggc gcccggatat ttttgcccgc tggccagctc accgcccagt tcgacaaaca
    10141 ggtccgtcgg cgccacccag cgcagctgca ccccgtcgtt ctgcagacgc ggcccgagga
    10201 aggccttgta ggcgaggggc gcgtcggtga agtcccactc gtggggatgc tgcacgttga
    10261 ggtagcccac gccggacagg aagcgcccgg ccttgagctt gaggccctcc ggcagggcca
    10321 gggtctcgat gtgtgcctct tccacctcga ccgtcccgcc ggcgccgtca tcggccaggg
    10381 agaagcgggc gttgccgcgg aacagatgat ccacgttggc ggagaccgcc agctcggatt
    10441 cgcccaggct gaagcccttt cgcggcggcc ccacttcgcc gcccgagggg atgaagccgt
    10501 cgaggcggta cttcgccgga tcctgctgga agcgcgtgaa cttgctgtcg agaatcagcg
    10561 acacggcggg gttgaaggcc ccttcgccgg tgggcggggt ggcgggcgcg atggccacgt
    10621 caggcagggc cgcggccttc tgctcggcct gctccgcact gcgggccgcc gcagtggcgg
    10681 tggtttcggc gcgcgctgcg cgggcctcgc tgtcggcaag gcgcttttcg agggcgtcga
    10741 tgcgttgctg gtaggtgtcg cgcagggcac gcagttcgtc gcgcagctgc ttcaggtcgg
    10801 cggcgctgtc ggccgcggac gcgacggcgg gcaaggccgc cgccagggcg acgcacagcg
    10861 cgcccggtcg tacgatggac atggtcggat ctcctcggat cggtccgtca agctcacagc
    10921 cggtcggcgc cagagcgccg gccggtcacg gggggaacgg ggtcagacgg gagaaacggg
    10981 cggatcgcgg ctcgacggcg tcggcgggcg gaggaaggcc ggcagggtgc cagcatcggg
    11041 cggaagggcc gaaacgggtg cttccggccc cggcagagac ggggtgaccg gcagggccgc
    11101 gcccagatcg gcggcagcca gacacagcag gcagcgctcg tggcgttcgg tcgccggctg
    11161 gccaccgctc gccggggcag cctgcgcatc gacgcgcaac ggacgctccg ctgcctcgaa
    11221 gtggccgatg gcatgcatcg ccccgagcag ttgggcgcac accagcaaca gcgccagcgt
    11281 gccgcggaac agggccgagg gccggccgcg gaagggcatg cgcctcacgc gacgagctcc
    11341 ggcacgcaga aacaggcgaa ggcgcgctcc accacggccg ggtcgagctc ccgcacgatg
    11401 aacaccaggc gggtctcccg cgcaccctgc ggccacgccg gcagggccgc atccggatag
    11461 cgcaggtgat ggacacactg gatcacccgc ggactcacct cgccggccac gtgcacgagg
    11521 cccttcaccc gcagcaggcg ctcgccccac aggctctgca gggcatccag ggccgccgag
    11581 aaatcgcccc acgcgacagg cgcgggcagg cggagcgcaa aggcacggac cgccgcatcg
    11641 tgctgtccgg cgggcgcaga gggcttcgcg gtcagcgggc ggtaatccgg atcgcgctgg
    11701 gcggcgaccg ccccgtcccc cagccagcgc gccacatcgg ccgagtgcgt gacgggattc
    11761 cacaggccac agtcgagcag atcggccgga tcgaccgcgc cacccacgct gcgcaggcgc
    11821 ggcgcaccgg ggttgaggcg cgccaggcgg gcgtcgaccg cgtcaagctg ggccggcgtg
    11881 gcctggtcca ccttggtgat gacgagccgg tcggccatcg ccgcctgctt gacggcctcc
    11941 ggccgcgcgg ccagctggtc ggcgccgaag acgccatcca cggccgtcac cacgccatcg
    12001 aggcggtagc gctcggcgac gaagaaatca ttcatcaggg tgaacagcac cggcgccgga
    12061 tcggccagac cggtggtttc gacgatcacc cggtcgaggg cagggagttc gcgacgcagg
    12121 cgccgcatga agagttcccg caggcagcgg gacaggtcgc cccgcacggt gcagcacagg
    12181 cagccggatt cgaggagcac gacctgctcg tccaggtgag tcaccagcag gtggtcgagg
    12241 gggatgctgc cgaactcgtt gatgaccacc accgtgccgg ccatgtcggg ccggcgcagc
    12301 agatgattga gaagggtggt cttgccggcg ccgaggaagc cggtgaggag ggtgacgggg
    12361 attcggtgtg cggcgcgcat cgggtcggtc atccgcgggg cgggtccgtc accatgcgac
    12421 ggcggggcgc cgacttattc cccgtacagc ttctgggtcc gttcccggcg ctcctgggcg
    12481 tcgatggaca gggtggccgt aggacgggcg agcaggcggc cgatgccgat ggggtcgccg
    12541 gtctcctcgc accagccgta ttcgccttcg gcgatgcggt tgagggcctc gtcgatcttc
    12601 ttcagcagct tgcgctcccg gtcgcggaca cgcagctcga gggcgtgctc ttcttcaacg
    12661 gtggcccggt cagccggatc ggcgacgatc tcgttctcgc gcagggaggc gcccgtgctg
    12721 gccgcattgt ccagcagctc ctggcgcatg cggctcaggc ggtcacggaa gaattccagc
    12781 tgcgccgggt tcatgtactc ttcttcgggc gccgccagaa tttcggcttc ggtcagaatt
    12841 ttgtccttgg tggtagcaac catgtctcgt tcctccttga ttcgacacgc ggatcccacg
    12901 ccagccgcgt cctccgggac gcagcctgtc acgcgtttcc agcaacgtac tgggcaacgg
    12961 cttcaccccc gacgcatccg ggtgccggtg catccggccg cgtctgtccg acgccggccc
    13021 gggcacaggg cgggattcta ggcacaggaa cgcggcaaat acaatcaccg cccccgttcc
    13081 gcccctacat tttgaaacag cgccggcacg cgccagaacc ggtacacgtc cccagctacg
    13141 gtaaaatgtc acaatgcatg ctcagaccga ccctgcccgc cccgcgcttt ccgagcagtc
    13201 ggtccgcccc tccttcaccc tccccgtgcg tgtctactat gaagacaccg atgccggcgg
    13261 ggtggtctat tacgccaact acctcaagtt ctgcgaacgg gcgcgcaccg actggctgcg
    13321 cagctgcggc atcggccagc accgcctggc cagcgaggac ggggtgctct tcgtggtacg
    13381 ctccgtccgc gccgactacc gccagcccgc cgtgctggat gacgacctgg ccgtcgtcag
    13441 caccctcgcc aagctcggcc atgccagcct gaccttccac cagaaagtcc tgcgcgacgg
    13501 cgtcgagctg ttcgacgccc aggtcgtcat cgcctgcatc gaccgcgatg cccgccgcgc
    13561 cattccgatt ccacccgctg tgcgcgcaca gttcgccacc ctggtttgac cccatgaacg
    13621 tcaccgaaga cctgtccatc ctgtccctga tcgccaacgc cagcctgctg gtcaagctcg
    13681 tcatggcgct gctcggcacc gtgtccttca tgtcctggta ctggatcttc cgcaagtggt
    13741 tccagatccg cgacgccaag aagaagaccg acctcttcga gcgcgacttc tggagcggcg
    13801 gcgacctcaa cgccctgtac cagtccgccg ccaccagccg ccacgaggcc gccggcatgg
    13861 agcgcatctt cgaatccggc taccgggagt tcgccaagct gcgcggcaag aatctggacc
    13921 acggcgccat catggacggc gccaagcggg ccatgcgcgc cacctaccag cgcgaggtgg
    13981 acgacctgga agcccacctc gccttcctcg cctcggtcgg ttcggtgtcg ccctacgtcg
    14041 gcctgttcgg cacggtgtgg ggcatcatga acgccttccg cgggctctcc aacgtgggct
    14101 ccgcgaccct cgcccacgtg gcgccgggca tcgccgaagc cctggtggcg accgccatcg
    14161 gccttttcgc ggcgatcccc gcggtggtcg cctacaaccg cttcgcccat gacatcgacc
    14221 gcatcgcgat ccgcttcgag agctttaccg aagagttcct gaacatcctc cagcgccagc
    14281 tgcgctgacc ggagcgaagt cgccatgcgt caacgccgcc tgatgaacca gatcaacgtc
    14341 gtcccctaca tcgacgtgat gctggtgctg ctcgtcatct tcatggtcac agcgccgatg
    14401 atccagtccg gctcggtcaa cctgcccagc gtggacaagg tgcccgcacc gcccagcgac
    14461 gccatgatcg tcaccgtcaa gcctgacggc gaactcaccc tcgcccagac cagccagtcc
    14521 cagccgcgca gctacaaccg ccgcgacctg atcggcgccc tgcaggatgc cacctcggcc
    14581 aacccgggcc agcccgtggt gatcgccgcc gacaaggacg tgaagtacga gcgcgtgatg
    14641 gacatcctca acgacctgcg caacgcgggc gtcaacaagg tcggcctgca gacgcagatc
    14701 ggcaagtcgg cccgatgaac cctgcgctgc ccggcaacga acggggcaag tggtcctccg
    14761 ccgccctcgc ggcgggggtc cacgtactgc tcggcctgtt cctgttctat ggcgtacgct
    14821 ggcagaaccg cctgcccgag ccggtcgagg tggagctggt gagcgccccg cccgtcacgg
    14881 caccggtcac cccgccgcca ccgccgccgg cccccgccga ggagcgccct gctccgcctc
    14941 cgccgccccg gccggaaccg gaacccgagc ccgcgccgcc gccacgcaag cagcccgaca
    15001 tcgccatcaa ggcgcccgag cccaggaaac tcccgcccaa gccggtgaag gaggtcgtca
    15061 agccggagcc gaaacccgag ccgaaaaagc ccgaacccaa gcctgagccc agaaagcccg
    15121 agcccaagcc cgaaccgaag aagccggacc ccaagcccga gccgaagccc gaacccaaac
    15181 cggatcccaa ggccgaagca aaggcccgcg ccgagcaggc cagactggac aaggccaagg
    15241 ccgacaagga cgccaaggcg gcctccgatc gcgcccgcga gcaagaggat cggcgcatgc
    15301 gcgaattgct cgcccatgac agcgagaatg ccaagcagtc cgcgcagatc caggaagagc
    15361 tggcacgcat cgccggtgcc aaggccagtg ccgcgcgcaa taaggccatc gaggcctaca
    15421 tcgagaagat ccgcgccaag gtcaagggaa acatcgtgct gccgccgggg gtggccggtg
    15481 atccctttgc cctgttttac gtggatcagc ttcccgacgg ttccgtgatc gaagtccgcc
    15541 tcaagcgctc caccggcacg ccgcagctcg actcggccat cgagcgggcc atccagaagt
    15601 ccagcccgct gcctcgaccg gacaagccgg agctcttcaa ccgcacgctg gaactcaagt
    15661 tctatcccct gagggactga gaaggccgcc cgaacggccc cgtcccgctc cattcacact
    15721 gatgacgtcc atccacccat gaaagcgacc ttcctgaccc tcctgcgcgg cctgctcccc
    15781 ggctgcgcac tggcgctggc cgccgcaacg gcccaggccc agctcgccat cgagatcacc
    15841 ggcgcaggcg ccaaccgcct gcccgtcgcc atcgccgcct tcgaaggcga ggcggccctg
    15901 ccgcgccccg tcaccgacgt ggtcaaggcc gacctggagc gcagcggcct gttcaaactc
    15961 atcgaactcg gccccacacc ccagggctac gacgcccagg tcaacctgcc cggcgtgcgc
    16021 agccgcggtg ccgatgccct cgcggtgggc agcgtggtga ccaccggcaa cggccgctac
    16081 gaagtccgcg tgcgcctcta cgacaccccc aagcaggccg ccctcggcgt gcaggcaatg
    16141 accatgtcgc ccgcccagta ccgcaccacc ggccaccgca ttgccgactt catctacgag
    16201 aagctcaccg gtgaaccggg cgtgttctcc acccgcatcg cctacgtcct caaatccggc
    16261 ccccgctacg aattgcaggt ggccgacgcc gacggcagca acgcccagac cgcgctggcc
    16321 tccaaggagc cgatcatctc gccggcctgg tcgcccgacg gcacgcgcct cgcctacgtg
    16381 tccttcgaat cgaagaagcc cgtggtgtat gtgcattccc tcgccaccgg tcagcgcaac
    16441 gtggccgcca acttcaaggg ctccaactcg gcgcccgcct ggtcgccgga cggccgccgc
    16501 ctggctgtgg tcctcaccaa ggacggccag tcccagctct acgccgtcaa tgcggacggc
    16561 tccggcgtgc agcgcctcgc cagctccgcc ggcatcgaca ccgaaccggc ctggtcgccc
    16621 gacggcgcgt ggatctactt cacctccgac cgcggcggca gcccgcagat ctaccgcatc
    16681 cccagcagcg gcggcgacgc ccagcgcgtc accttcgagg gcagctacaa cgtgagcccg
    16741 cgccccgccc ccgacggcag caatctcgtc tacgtgtccc gcaatggcgg ccgcttccag
    16801 gtcacgatga tggatctcaa ttcccgccag acccagatcc tcaccgactc gtccaaggac
    16861 gaatccccca gcttcgcccc caatggccgc atgatcctct acgccaccga gatcggcggt
    16921 cgcggcgtgc tcgccgccgt gtcgtccgac ggtcgtgtca agcagcgact gtcggtacaa
    16981 tccggcgacg tacgcgaacc cgcctggggc ccgcaacctc gcaactgacc gatctgaaac
    17041 cacgcgccca taagctggtc attccattaa cccgcccctt gcgaccgctc acggagtccc
    17101 ccatgaacat cccccgcttc gccctcaccc tcgccgccgc cggcatcctg gccgcctgct
    17161 cctcaagccc caagaccggt gacgagcagt ccggtgccgc cgtcgaagac cgctccggcg
    17221 ccgccagctc cggcatcgac agcagccgcg tggtccccgt caccccgggt gacgccgccg
    17281 gcgccggcat cgccgccctc aaggacccca aaagcatcct ctccaagcgc agcgtctact
    17341 tcgactacga cagctacgtg gtgaaggacg aattccgcgc cctcgtggaa gcccacgcca
    17401 agttcctggt cggccatccg cagatgaaga tgctcatcca gggcaacacc gacgaacgcg
    17461 gcagccgcga gtacaacctc gccctcggcc agaagcgctc cgaagcggtc aagaaggccc
    17521 tgtccctgct tggcgtgccc gaaagccagc tcgaatccgt cagcctcggc aaggaaaagc
    17581 ccagctgcag cgacgccacc gaaacctgct ggtctgccaa ccgccgcggc gatatgctct
    17641 attcgggtga gttctgatga agcagcgcct tctgcccctt ctgctggccg ggctggtggt
    17701 cgggctggcg ccgctgcggg cccacgcggg gctcttcgac gacgacgagg cccgcgcccg
    17761 catcgaggac atgcgcaagc agctcagcgc ccgcatcgac aagctcgaag ccagcgcccg
    17821 cgcccagctt gaactggcca accagatcga acagctcaag gccgagatcg cccgcctgcg
    17881 cggcgagaac gaagttctgg ccaacgatct ggccaacgcc aacaagcggc agaaggactt
    17941 ctacgtcgac ctggacaacc gcctgcgcaa ggtcgagccg cccgtcgccg ccgcggccga
    18001 cccggctgcg acgcctgtgc agccggcgcc gcagccccct gcagttgatc cggccaacga
    18061 gaaccgcgac tacgaagcgg ccctcaacca gctgcgcggc ggcaagtaca aggaagccgc
    18121 cacagcgttc accgccttcc tgcgcaatta cccgaacagc agcttccagc ccagcgccca
    18181 ttactggacc gccagcgccc agtaccagct caaggactac gccaacgccg ccgaccacta
    18241 cggtcgcgtc gccagccagt ggcccgacga cacccgcgcg ccggacgccc tgctcggtct
    18301 ctccagcgcc cagtccggcc ttggcgacct caaggccgcc acccgcagcc tggaaaagct
    18361 cgtcgccaag taccccaaca gccaggcggc ccagatcgcc cgccagcgcc tcaaaaagta
    18421 agccgacgcc ccgtgcccca tcgggccggg gcgcacccgc ccccgcatcg cccatgaaca
    18481 ccccctccgg ccaccccgtc accctgcgca tcaccgagat cttccactcc ctgcagggcg
    18541 agacctcccg cgccggcctg cccaccgtct tcgtacgcct caccggctgc cccctgcgct
    18601 gcacctggtg cgacacgacc tactcattca ccggcggcac gcgccgcagc ctcgacgacg
    18661 tcctggccga aatcgccgcc tacgcgtgcc ccaccgtgtg cgtcacgggc ggcgagcccc
    18721 tcgcccagaa gggttgtctc gcgctcctca ccgccctgtg cgacgcgggc tattccgtct
    18781 ccctcgagac gagcggcgcc ctcgacatcg ccgacgtgga tccgcgcgtc tcacgcatcg
    18841 tcgacctcaa ggccccgggc tccggtgaag aagcgcgcaa tctgctgagc aacctggacc
    18901 acctcgcgcc gcaggacgag ctcaagctgg tcctcgccag cgaggccgat tacctctggg
    18961 cccgccacat gctcgccgaa caccggctgg ccgaacgctg cccggtcctc ctcgccccgg
    19021 tccagggcca gcttcccccg gccgatctgg ctgcctggat cctgcgggac cgcctgcccg
    19081 tacgcatgca ggtccagctt cacaaggtca tctggggcaa cgaaccgggc cgctgacgcc
    19141 ccctgttccc gattggtcgt tcgcgttacc ctgaccgcct cgccaggagc tcccatgaac
    19201 accccacgcc cccgcgccat cgtcctgctg tccggcggac tcgactccac cacctgcctt
    19261 gcgatcgccc gcgccgaagg cttcgacgcc tatgccctgt ccgtcgaata cggtcagcgc
    19321 catgccgccg aactggccgc cgcgcgccgc atcgccgccg cgctcggcgc catcgaccac
    19381 cgggtcgccc acgtggatct gggccagttc ggcggctccg ccctcacgga cccggccctc
    19441 gcggtgcccg tggacggcgt cgccgggggc atccccatca cctacgtgcc ggcgcgtaac
    19501 accatcatgc tgtccatcgc cctggcctgg gccgaagtac tcggcgccga cgacatctac
    19561 gtaggcgtca acgcggtgga ctactcgggc tacccggact gccgcccggc ctacatcgac
    19621 gccttcgaga aaatggccaa tctcgccacc aaggccggcg tcgaaggcca tcgcctgcgc
    19681 atccacgcgc ccctgatcga cctttcgaag gccgacatcg tgcgccgcgg cgcggcactg
    19741 ggcgtggact acggactcac cgtgtcctgc tatcaggctg ctgacgacgg cgcggcctgc
    19801 ggcctgtgcg acgcctgccg cctgcgcagc gccggcttcg cggccgccgg cctgcccgac
    19861 ccgacccgct accgctgatc gccccgccga ccgttcgggc ttgacgatca tgtttccgct
    19921 gctataatcc gcgtctctct gcgcgggtcg ttagctcagt tggtagagca gcggactttt
    19981 aatccgttgg tcgcaggttc gaatcctgca cgacctacca ctgatccaaa aattgggtta
    20041 gaatgcgcga ctcgcagcac acaatgggtt gttagctcag ttggtagagc agcggactct
    20101 taatccgtag gtcgagtgt
//
LOCUS       DAOQPA010000061        19959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1335_length_19959_cov_19.445388, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000061 DAOQPA010000000
VERSION     DAOQPA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19959
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_19959_cov_19.445388"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKX01_09905"
     CDS             <1..320
                     /locus_tag="PKX01_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HMZ76482.1"
                     /translation="AAQTLHPVAGQGYNLALRDVWACAQVLLRSGGDPGAPETLATYA
                     RERRLDRAGTIRFTDTLVRLFSTDSAPLRHARGAGLFALDLLPPARHFVAKRMMFGAR
                     AWP"
     gene            441..1883
                     /gene="mgtE"
                     /locus_tag="PKX01_09910"
     CDS             441..1883
                     /gene="mgtE"
                     /locus_tag="PKX01_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HMZ76483.1"
                     /translation="MSETDDLQHHDDLDRHVREVQHLLARHKLVEDLVHRQDMPRHDL
                     VENLVHKQHVNELTHKLDSLHPADIAHILEALPLDERLFVWDLVKAERDGEILLEVSD
                     AVRESLIESMDTAALQAAAEQLDADELADLAPDLPHEVMEAVYQGLAQEEREQLRAAM
                     SYPEDSVGALMDFEMVTVREDVTLEVVLRYLRRFEALPDHTDQLFVVDRDERLRGVLP
                     LNRLLLNDPDVTVSDVMLTDTLTLEPADEAETAAQAFERYDLVSAPVVDYHRKLVGRV
                     TVAAVVDFIREETENELLSQAGLKEGEDIFAPVWDSVKNRWSWLAVNLVTAFIASRVI
                     GAFENSIEKLVALAALMPIVAGIGGNSGNQTITMIVRAIAMGQVQHDALPRLLKKELG
                     VALLNGLVWGGLLGVLAWILYGSGKLGAVMTAAMTLNLLVAAFVGVMAPMIRHRFGQD
                     PAVGSSVIITAVTDSGGFFIFLGLATIFLM"
     gene            1988..2707
                     /locus_tag="PKX01_09915"
     CDS             1988..2707
                     /locus_tag="PKX01_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HMZ76484.1"
                     /translation="MKLAATLATLALSFNAGAAEFNAPWNDAQIALVIDPYAANSLKW
                     DALAGEPRVVAIIHKATIGTQGLDKAYWTRKAEARQRGYLWGSYHWGVAGDPEKQADY
                     YLDTVKPGDDELIALDLEDATSKTLMSAAEAVRFIERIHARSGRYPVLYTNHASAVLI
                     SKTYKNSVFAHTPLWYARFKTQVTDFPKGLWPTYTLWQFSSEIKPQKAIPGTRSDMDV
                     NVFNGSVEQLRTAWPLTQRAP"
     gene            complement(2735..4021)
                     /gene="hemL"
                     /locus_tag="PKX01_09920"
     CDS             complement(2735..4021)
                     /gene="hemL"
                     /locus_tag="PKX01_09920"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HMZ76485.1"
                     /translation="MTSRNEALFARAQRTIPGGVNSPVRAFRSVGGTPRFLQRAQGAR
                     VWDADGKEYLDYVGSWGPAILGHADPVVVEAVRAAALDGLSFGAPTEREIEMAERLCE
                     LLPSLEMVRLVSSGTEATMSAIRLARGFTGRDAIVKFEGCYHGHADSLLVKAGSGLLT
                     FGNPSSAGVPEDFAKHTVVLDYNDPAQLEETFKARGNEIACVIIEPIAGNMNLVKPSA
                     EFMHLLRRLCTEHGAVLIFDEVMTGFRVGPRGVQGLFGITPDLTTLGKVIGGGMPVGA
                     FGGRRDIMEKIAPLGAVYQAGTLSGSPVAVAAGLASLNQIARPGFYDGLTARTLRLVQ
                     GLAAAAAQHGVRFTADAVGGMFGLYFAPAVPTSYADVMASDRETFNRFFHAMLDAGHY
                     FAPSAFEAGFVSAAHTDADIDSTIATAAAWFAANGG"
     gene            complement(4039..4671)
                     /gene="thiE"
                     /locus_tag="PKX01_09925"
     CDS             complement(4039..4671)
                     /gene="thiE"
                     /locus_tag="PKX01_09925"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HMZ76486.1"
                     /translation="MAKTDPVLRRGLYLVTPDWTDTGRLVAMLPALLATRPALLQYRN
                     KLADAALRRVQVAGLLPLCRAAGVPLVINDDLALALECGADGVHLGRDDGDLAAARAA
                     LGPHRLLGVSCYAEWPRAEAAAAAGADYLAFGAMFPSSTKPSAPPAPLALLGRAKAAF
                     GRPVAAIGGITVDNAPLLRAAGADLLAVISDVFSAPDPAARAAAYGRLFD"
     gene            complement(4658..5497)
                     /locus_tag="PKX01_09930"
     CDS             complement(4658..5497)
                     /locus_tag="PKX01_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HMZ76487.1"
                     /translation="MISTRSDPSPPLVMVFAASDPTGGAGIQADIMTLSGMGCHALTV
                     ITALTVQDTAGVEAVLPTDAEFVDEQARALLEDMPVQAFKLGVLGSLDNIEVIARILA
                     DYPHVPVVVDPVIASGRGDELASESMLEGLCELILPQATVVTPNTLEARRLAARHRDD
                     DDTPTLAECARRMVAMGCEYVLITGSHEHSVQVINTLYGRGGVVRADTWERLPGAYHG
                     SGCTLASAVAAALARGLEVPVAVRDAQEFTWQTLAYAFRPGMGQHIPDRFFWARPVDD
                     GED"
     gene            5613..5765
                     /locus_tag="PKX01_09935"
     CDS             5613..5765
                     /locus_tag="PKX01_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMZ76488.1"
                     /translation="MCLICGYIYDEAAGAPNEGIAPGTRWEDIPPNWTCPECGARKDD
                     FEMVAI"
     gene            5829..6224
                     /locus_tag="PKX01_09940"
     CDS             5829..6224
                     /locus_tag="PKX01_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76489.1"
                     /translation="MNLNGIKVMVIDDSNTIRRSAEIFLAQAGCHVVLAEDGFDALAK
                     IADHAPDLIFVDIMMPRLDGYQTCSLIKKNARLKSTPVIMLSSKDGLFDRARGRLAGS
                     DEYLTKPFTKESLLRSVASHVPPRAGAAN"
     gene            6246..6611
                     /locus_tag="PKX01_09945"
     CDS             6246..6611
                     /locus_tag="PKX01_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76490.1"
                     /translation="MPIKKILVVDDSPTERLALSEVLVRHGYQVVTADSGDEAVIKSK
                     TEQPDLILMDVVMPGTNGYQATRTIVRDEQTRNIPVIICTSKGLETDKIWGMRQGAFD
                     YLVKPLDHAMLLDKIKALG"
     gene            6619..7134
                     /locus_tag="PKX01_09950"
     CDS             6619..7134
                     /locus_tag="PKX01_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ76491.1"
                     /translation="MAKRISLQEFQAGLARRLAEARDTRASALLAVQAGSRNWLLDLA
                     EAGEILAVPPLAPVPLVHPWFRGLASVRGNLFGVVDFAAFHGEAPVVTGGQARLLLVG
                     ARLNLNCALLVSRALGLRSVDDFEPLSEPEDATTPWIGAQLQDAHGHTWTRLRVQGLL
                     NHSRFLDAALT"
     gene            7221..9305
                     /locus_tag="PKX01_09955"
     CDS             7221..9305
                     /locus_tag="PKX01_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76492.1"
                     /translation="MATNTGDNTILEASERGEVSAVLPFLAGKSTAEQLRLLRLPFIA
                     FAVLTAVLVIYQIRVSSFGTAYVTAAGQMRTLSQQMAKASQLALQGEAGAFAELKRSR
                     EQFNHLLEAVTQGGDVDGTGVPGGSSAVADELTAVTELWQKSDKDAGQLLGQEKNLAS
                     LGNAVATINTKNAHLLELTEQVAALKLQTGASAREIATANQVVMLTQRIAKNANALLV
                     ANAIDPEVAFLLGKDTNTFREQIEALMKMTAAGSDAQSKLVELEGVAKDNLAAVQAIL
                     GNIQLLVQAKQSGSNIFQNSGPLLEKTSALAEGYSRAYTGFFTWVTVGIMLFAVLSIV
                     LLLLMAKVYNDDIARRRFSAERLRESAESERNSTQQAILRLMNEMGDLADGDLTVRAT
                     VTEDITGAIADSVNYTIEELSVLVRRINDAASRVTSATESAQRTSDELLAASERQAQE
                     LRLAGDTAHSMAVSMSEASEGALQSAQVARRALEAAQKGATAVEDTIKGMNDIRDKIQ
                     DTSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEV
                     QRLAERSAEATKQIGAIVKTIQTDTADAVAAMENATRDVVDGASLSETAGRELAEIER
                     ISAETAELIERISVSTEIQAKSATQVAEKMKGILAVTDRTTAGTQQTAVSIGELADLA
                     VELKGSVSGFKV"
     gene            9349..15006
                     /locus_tag="PKX01_09960"
     CDS             9349..15006
                     /locus_tag="PKX01_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ76493.1"
                     /translation="MSSAHELDLGPLTWVKGEIDLALERAAGALAEAETASDRLGRIK
                     FAQTHLHQAHGALSIVGLDGLTQFSEALDRLLGELAAEQVTYTPAIARLATRGLAALG
                     NYLNELTRGQPDQPLRLFELYAQLVEARGHEAPGPSDLFYPDLSLRPAFAPLPEGGTD
                     DARTLRALRGRFERGLLKWLRNPGDASGPAEMRDAVNGIERTQRQPASRAFWLASAAF
                     FDALGQQAIDVDPPLKRLCGRIDAQMRRLLEGSAVVAERLMRDVLYQVAIARCNTPPV
                     NTLREAYRLVNLIPAPGRSVSDAPVAPLLVALHSGLGDAREAWTLFTGGGAIGLPRFQ
                     DALAPLPATARAIGQPHTAALIEGLGRLAQWLRQDPLRLTDTLSMDMATAMLLVEGLV
                     DSHEMPSPGPRLKKQIAAVTERIQAYLRGEQPAPLSIADPDTADQQLGRQLAHEILAN
                     LGDVEQHLDGFFREPARTDLLAATSAPLKQVEGALAIMNQGAAVRLVQDCAAAISALE
                     SGRQSPEAAIFEDIAHRLSALGVFIDALQHGPADLEQILNPSTGDSAGDSLEAELVQQ
                     KRETAALAAALQENPDDIGLRTELLLNLEGLRQDASLTADAELEKQAREALNLLDAGA
                     PFAAPPAIVPEAMAPALSPEAARLLDASDAEIDAELLDIFIEEASTVRDTIASHLDLL
                     IAAPGDQEHLTTIRRSFHTLKGSGRMVGLKDFGEAAWGMEQTLNHWLRLERPATPELL
                     ALIRRAHVAVSDWVDQLQAGGGLSLDVADILREAERLRGEEDHGAALEIVQNLESSIL
                     QPIIELDAPPPAPAVEFPALDFDSLEASGERPSTDRLDIVLDDAPAEPPVALEELVWA
                     EPPAVPAPPAPTAAEPAPSELATDAIEIEIEIEAAALEDDDVFPEEGAEVIDLGAPEP
                     LEPDASEAVDLPESDATAEAIELGAAAPESVADAPSHGREHDTEDIDSLEDDDAVRLG
                     HVVLSRPLFDLYLAEARQHLATLRHELGRMAVNPSLLPANAAIRAAHTLSGISGTAQF
                     TAPQSLAKALELAQQRLADHHQAPSADQAQLLNGAATALETMLAQLSGRRLPDAQPEL
                     VDALDNVLRGHLAEVITLRPEIPSDLPPPELATAPLARSPEPVTPPPEAPSAAAPVLR
                     DELDAELLPIFLDEGNELLHSLDTALRAWRAQPDDATQPAAVARLLHTLKGSARMVGA
                     MSFGASIHELETRLERARRLAPDDIAIVDDIESALDRSRQTLETLGISPPATTPAAVA
                     AAPIDVETGAVEAVPAGADGTLAAPRAQLRVEATRVDQFVNEAGEISIARTRIEGELR
                     TLRRSLLDLTENVIRLRNQLREVEIQADTQMQSRLSQVETRHTEFDPLEMDRYTRLQE
                     LTRMMAESVGDVTTVQQNLLRNLDGAESALHAQSRMTRDLQQSLMHVRMVPFDSLAER
                     LYRLVRQTAKDVGRRANLDIIGGQIEIDRGVLEAMTGPLDHLVRNAVAHGIELPDARR
                     AAGKAEIGQITLRITHEGNEVVIEFRDDGAGLDFDAIRAHAIATGLLGTDEESDDKRL
                     ANLIFLPGFSTATLSSVAGRGVGMDVVKSETAAVGGRLSVETDRGRGTTFRIHLPLTL
                     AVTQALLVRSGGRTWAIPSSMVAQVLELKAAALEKVRAEHGTDWLGERFAYRYLPRLL
                     GDSATQPQVERFNWVLLLRAGSETLALHVDALRGNQEVVVKNAGPQYARMVGFSGATV
                     LGDGEIVLILNPVALAARAPDATDDGVPGLPAAPAIPEVPLVMVVDDSLTVRKITGRL
                     LEREGYRVLTAKDGVDALEQLGHNDPDVIIADIEMPRMDGFDLTRNIRADARHKRVPI
                     IMITSRMAEKHQRYAEEIGVNHYLGKPYDEDLLLRLIRRYTRA"
     gene            15084..17042
                     /locus_tag="PKX01_09965"
     CDS             15084..17042
                     /locus_tag="PKX01_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HMZ76494.1"
                     /translation="MNLLFEEDGAFKTGTVLTDNDSTLQVETPHGKRLKLKSSHVLLR
                     FPQPAAGELLDRAEIEAEGLDTEFLWDVCGDDEFGFADFAREYYGHAPTPIEATAVLL
                     RLHAAPIYFHRKGRGRFRKAPADILQAALAGLEKKRQQAAAIERMRDALLAGTLPEEF
                     RPLIRPLLFKPDRNRLETKALEAACAECGKSAARLLLDCGALASSQELHFDRFLYEYF
                     PEGTAFPSVDAPTAPADLPLAPVRAFSIDDAATTEIDDAFSVTATPDGWRIGIHIAAP
                     GLGFGRDSALDAIARTRLSTVYMPGHKITMLPDVVVEGFTLGEGRDCPALSLYLELAR
                     DLRITGHHSALERVPVAANLRHHDLEPVFNETTLADGGPDFPWKAELTLLWELATVLE
                     AGRGKPAANQTLVDYAFNVDWSVATPDGPGRVDIGRRPRGSPLDKLVAELMICANSTW
                     GKALADAGVPALYRAQTGGKVRMTTSAAPHEGLGVDCYAWSSSPLRRYVDLVNQWQLI
                     AWLRGEAPAFPPKSADLIAAMRDFELTYAAYADFQRGMERYWCVRWLRQIERPELTAR
                     VLRESLVRLEDIPLTFKLPSMPTLPSGTRVRLAIDSTDLIDVEVRATYLETLADAPLE
                     MAEALAEDVTDAPLPEAAPPASDAPPAS"
     gene            17106..18005
                     /locus_tag="PKX01_09970"
     CDS             17106..18005
                     /locus_tag="PKX01_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ76495.1"
                     /translation="MAAGRLPLVAPSRKASRYALEWGVGLSLLLHALLLSVHFVMPDA
                     NHFTRTHQDLAVVLVNARHAQRPDKAELLAQTTLDAGGTSTPDARASTPLPPQTLSRA
                     GDGLVEAQRRAETPQTPQMQAMTAPSGKTALAVDSRQTAPTEPPRQVSGYDLLDSSAA
                     VARIEAQIDKDLVDYAARPRKKFIGARTQEYRFAQYVEDWRQKIERVGTLNYPEAARG
                     KVYGSLLLSVTIRSDGTVVSIDVHRSSGHKVLDDAARRIVQLAAPYAIFPPDIRRDTD
                     VIEITRTWTFTNSDMLQTSDKKE"
     gene            18011..18841
                     /gene="aroE"
                     /locus_tag="PKX01_09975"
     CDS             18011..18841
                     /gene="aroE"
                     /locus_tag="PKX01_09975"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMZ76496.1"
                     /translation="MDRYAVIGNPIAHSKSPRIHALFAAQTAQALSYEALLGPLGAFA
                     ATVDAFRAAGGRGLNVTVPFKLDAFAYATRRTPRAEAAGAVNTLAFGPDEVLGDNTDG
                     AGLLRDITVNLGCPLTERRVLLLGAGGAARGSLLPLLLTEPARLVVANRSSAKAVALA
                     AEFARLPSCAVAVKLEGCGFADLAGAQFDVVINATSASLGDAAPDLPAGLYAPGSLAY
                     DMMYGKGDTPFLAAARAQGAGRLADGLGMLVEQAAESFALWRGVRPDTAPVLAALRAG
                     "
     gene            18864..19556
                     /gene="mtgA"
                     /locus_tag="PKX01_09980"
     CDS             18864..19556
                     /gene="mtgA"
                     /locus_tag="PKX01_09980"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HMZ76497.1"
                     /translation="MKTFGRWLKRGLLGLVLVFLAWHLWLLGHVLWWKWQPPSETSFM
                     SLRLDELATRRPDAELRYRWVPYERISVYLKRAVVAAEDDKFTDHEGFDWDGIQKAIE
                     KNQKKGRAVAGGSTISQQLAKNLFLSPARSYLRKAEEALITVMIETFWDKRRILEVYL
                     NVVEWGDGIFGCEAAAQRYFHISAAQLGPAQAARLAVMLPNPRKYEKDFGPRLAAHAA
                     RIQARMQYSDVP"
     gene            19646..>19959
                     /locus_tag="PKX01_09985"
     CDS             19646..>19959
                     /locus_tag="PKX01_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HMZ76498.1"
                     /translation="MQFAGFTLRNNLFVAPMAGVTDRPFRQLCKKMGAGLAVSEMVTS
                     NSLLYGSAKTQRRANHDGEVDPISVQIAGADPAMMAEAARHNIDRGAQIIDINMGCPA
                     KKV"
BASE COUNT         3437 a         7570 c         6101 g         2851 t
ORIGIN      
        1 acgccgcgca gaccctccac ccggtcgccg gccagggcta caacctggcc ctgcgtgatg
       61 tatgggcctg cgcccaggtg ctgctgcgca gcggcggcga tcccggcgcc ccggagaccc
      121 tcgccaccta cgcccgcgaa cgccgcctcg accgggccgg cacgatccgc ttcaccgata
      181 ccctggtgcg cctgttcagc acggattcgg cgcccctgcg tcacgcccgc ggcgccggcc
      241 tgttcgccct ggatctgctg ccgccggcac gccatttcgt ggccaagcgg atgatgttcg
      301 gcgcccgcgc ctggccgtga gcccggcggg ctgaagtccc gcccggcgcc ctgcacgtcc
      361 gcctccccgc ggcgcagcaa acgccgtaga atccgcccct ccgcgtcccc atccgggggc
      421 ctcgtgccga gtggaccgcc atgtccgaaa ccgacgacct gcagcaccac gacgacctcg
      481 accgccacgt gcgcgaggtg cagcatctgc tcgcccggca caagctggtg gaggatctcg
      541 tccatcgcca ggacatgccc cgccacgatc tggtggagaa cctggtccac aagcagcacg
      601 tcaacgagct cactcacaag ctcgacagcc tccacccggc ggacatcgcc cacatcctag
      661 aagccctgcc cctcgatgaa cgtctcttcg tctgggatct ggtcaaggcc gagcgggacg
      721 gcgagatcct gctggaagtc tcggacgcgg tgcgggaatc cctcatcgag tccatggaca
      781 ccgccgcgct gcaggccgcc gccgagcagc tcgacgcgga cgaactggcc gacctagcgc
      841 cggacctccc ccacgaggtc atggaggcgg tgtatcaggg cctcgcccag gaagaacgcg
      901 agcaactgcg cgccgccatg tcctatccgg aggactcggt cggtgcgctg atggacttcg
      961 aaatggtcac ggtacgcgag gacgtgaccc tggaagtcgt gctgcgctac ctgcgccgct
     1021 tcgaggcgct gccggaccac accgaccagc tcttcgtggt ggatcgggac gaacgcctgc
     1081 gcggcgtgct gcccctcaac cgcctgctcc tcaacgaccc ggacgtgacg gtgagcgacg
     1141 tgatgctcac cgacaccctc accctggagc cagccgacga agccgaaacc gcagcccagg
     1201 ccttcgagcg ctacgacctg gtgtcggcgc ccgtcgtgga ttaccaccgc aagctggtgg
     1261 gccgcgtgac ggtggcggcg gtggtggact tcatccgcga ggaaaccgag aacgagctgc
     1321 tcagtcaagc cggtctgaag gaaggcgaag acattttcgc gccggtgtgg gactcggtga
     1381 agaaccgctg gtcctggctg gccgtcaacc tggtcaccgc cttcattgcc agccgcgtca
     1441 tcggcgcctt cgagaactcc atcgaaaagc tggtggccct cgccgcgctg atgcccatcg
     1501 tcgccggcat tggcggcaac tcgggcaacc agaccatcac catgatcgta cgcgccatcg
     1561 ccatgggtca ggtccagcac gacgccctgc cgcgcctgct caagaaggaa ctgggtgtgg
     1621 cgctgctcaa cggcctggtg tggggcgggc tgctcggcgt gctggcgtgg attctctacg
     1681 gcagcggcaa gctcggcgcg gtgatgaccg ccgccatgac cctcaacctg ctggtcgccg
     1741 ccttcgtggg cgtgatggcc ccgatgatcc gccaccgctt cggccaggac ccggcggtgg
     1801 gctccagcgt catcatcacc gccgtcacgg attccggcgg cttcttcatc ttcctcgggc
     1861 tggcgacgat tttcctgatg taagcaccgg cccggggcca gcggcaacgc gcgctggaaa
     1921 agcgggccca cggcgactac ggtgacagga acgcccgtcc ccgttgccgc ccggaaaggt
     1981 tcacgccatg aagctcgctg ccaccctcgc caccctggcg ctgtccttca acgccggggc
     2041 tgcggaattc aacgcgccct ggaacgatgc ccagatcgcc ctcgtcatcg atccgtacgc
     2101 cgccaacagc ctgaagtggg acgccctggc cggcgaacca cgggtcgtcg cgatcatcca
     2161 caaggcgacc atcggcaccc agggcctcga caaggcctac tggacccgca aggccgaagc
     2221 gcgccagcgg ggttacctgt ggggctccta ccactggggc gtggccggcg atccggaaaa
     2281 gcaggcagac tactatctgg atacggtcaa gccgggcgac gatgaactga tcgccctcga
     2341 cctggaagac gccacgtcca agaccctcat gtcggcggcc gaggcggtcc gcttcatcga
     2401 gcgcatccac gcccgcagcg ggcgctaccc ggtgctctac accaaccacg ccagcgcggt
     2461 gctgatctcg aagacctaca agaacagcgt ctttgcccac acgcccctgt ggtacgcgcg
     2521 cttcaagacc caggttacgg atttcccgaa gggtctgtgg cccacctaca cgctgtggca
     2581 gttctccagc gagatcaagc cccagaaggc gatccccggc acccgcagcg acatggacgt
     2641 gaatgtcttc aacggcagcg tcgaacagct ccgcaccgcc tggccgctga cacagcgcgc
     2701 gccctgaggc cgggccgcgc gccgggcacg gtgctcagcc gccgttcgcc gcaaaccagg
     2761 cggccgccgt ggcaatggtg gaatcgatgt ccgcatcggt gtgcgctgcc gacacgaagc
     2821 ccgcctcgaa ggccgacggc gcaaagtagt gaccggcgtc cagcatggcg tggaagaagc
     2881 ggttgaaggt ctcccggtcg gaggccatca cgtccgcgta ggacgtcggc accgccggcg
     2941 cgaagtacag cccgaacatg ccccccaccg cgtcggcagt gaagcgcacg ccgtgctgcg
     3001 ccgccgccgc ggcgagaccc tgaacgaggc gcagggtgcg cgcggtgagg ccgtcgtaga
     3061 agcccggccg ggcgatctgg ttgaggctgg cgagccctgc cgccaccgcc accggactgc
     3121 cggacagcgt ccccgcctgg tacacggcgc ccagcggggc gatcttctcc atgatgtcgc
     3181 gacggccgcc aaaggcgccc accggcatgc cgccgccgat caccttgccc agggtggtga
     3241 gatccggcgt gatgccgaag aggccctgca cgccccgcgg ccccacacgg aagccggtca
     3301 tcacctcgtc gaagatcagc accgcgccgt gctcggtgca caggcggcgg agcaggtgca
     3361 tgaactcggc cgagggtttg accagattca tgttgccggc gatgggctcg atgatcacgc
     3421 aggcgatctc gttgccccgc gccttgaaag tctcttcgag ctgggccggg tcgttgtagt
     3481 cgagcacgac ggtgtgcttg gcgaaatcct cgggcacgcc ggcggaagac gggttgccga
     3541 aggtcagcag gcctgagccg gccttcacca gcaggctgtc ggcgtggccg tggtagcagc
     3601 cctcgaactt gacgatggcg tcgcgcccgg taaaaccgcg ggccaggcgg atggcgctca
     3661 tggtggcttc ggtgccgctg gaaaccagcc gcaccatctc caggctgggc agcagctcgc
     3721 acaggcgctc ggccatctcg atctcacgct cggtgggcgc gccgaaggac agcccgtcca
     3781 gcgccgcggc acgcaccgcc tcgaccacta ccggatccgc gtggccgagg atggcgggcc
     3841 cccaggaccc gacgtagtcg agatattcct tgccgtcggc atcccacacc cgcgccccct
     3901 gggcgcgctg caggaagcgg ggcgtgccgc ccaccgagcg gaaggcccgg acgggcgaat
     3961 tgacgccgcc gggaatggtg cgctgggcgc gggcaaagag tgcttcgttg cggctggtca
     4021 tacggacgat cctggaattc agtcgaaaag ccggccatag gccgccgcgc gggcggccgg
     4081 gtcgggcgcg gaaaacacgt cgctgatgac ggcgagaaga tcggcccctg ccgcccgcag
     4141 caagggcgca ttatcgacgg taatgccgcc gatggcagcc accggccgtc caaaagccgc
     4201 cttggcgcgg ccaagcagcg ccagcggcgc cggcggcgca gagggcttgg tggacgacgg
     4261 gaacatggcg ccgaaggcca gatagtcggc gcccgcggcg gcggcggcct ccgcccgggg
     4321 ccattctgcg taacagctca cgccgaggag acgatggggc ccgagggcag cccgggcggc
     4381 ggcgaggtcg ccgtcgtcgc gccccagatg gacgccgtcc gcgccgcact ccaatgccag
     4441 cgccagatcg tcgttgatca cgaggggcac gccggcggcg cgacacaggg gcagcaggcc
     4501 agccacctgg acgcgccgca gggccgcatc ggcaagcttg ttgcggtatt gcagcaaggc
     4561 cggtcgggtg gcgagcaagg cagggagcat cgccacgagc cgcccggtgt cggtccagtc
     4621 gggagtgacc aggtagaggc cgcgccgcag cacgggatca gtcttcgcca tcgtccaccg
     4681 ggcgggccca gaagaagcgg tccgggatgt gctgccccat gccggggcgg aaggcgtagg
     4741 ccagggtctg ccaggtaaat tcctgagcgt cgcgcaccgc cacgggcacc tccagccccc
     4801 ttgccagggc tgcggccacc gccgacgcga gggtgcagcc cgagccgtga taggcgccgg
     4861 gaagccgctc ccacgtatcg gcgcgcacca cccctccgcg cccgtacagg gtgttgatca
     4921 cctgcacgct gtgctcatgg gaacccgtga tcaagacgta ttcacagccc atggccacca
     4981 ttcggcgggc acattcggcc aaggtggggg tgtcgtcgtc gtcgcgatga cgggccgcca
     5041 ggcggcgggc ctccagcgta ttgggagtga ccacggtggc ctggggcagg atcagctcgc
     5101 aaagcccttc gagcatcgac tcggaggcca gctcgtctcc ccgcccggag gcgatcaccg
     5161 ggtccaccac caccgggacg tggggatagt cggcgaggat gcgggcgatg acctcaatgt
     5221 tgtcgaggct gcccagcaca ccgagcttga aggcctggac gggcatgtcc tcgagcagcg
     5281 cccgggcctg ctcgtcgacg aattcggcat cggtggggag gaccgcctcg acaccggcag
     5341 tgtcctggac cgtcagggcg gtgatgaccg tcaacgcatg acaccccatc ccggagaggg
     5401 tcatgatgtc ggcctggata cccgcgccgc cggtgggatc ggaggcggca aacaccatga
     5461 cgagtggagg agagggatcg ctacgggtgg aaatcatcgg agcatgcgag gacgcacaaa
     5521 ttttacaacg actgcgctac cattcacgat ccaagcgatt ctagcccaat tcaccccgct
     5581 cccacatgct cacccccgaa tacaaaacct ggatgtgtct gatctgtggc tacatctacg
     5641 acgaggcggc cggtgcgccc aacgagggta ttgcccccgg aacgcgctgg gaggatattc
     5701 ctcccaactg gacctgcccc gaatgcgggg cgcgcaagga cgactttgaa atggtcgcca
     5761 tctgaacctt caaaacatga catcatgata tacccaaatg tcatgcaatc cgagcccgga
     5821 gaatcaaggt gaacctgaac ggcatcaagg tcatggtcat cgacgacagc aacaccatcc
     5881 gtcgcagcgc cgaaatcttc ctggcgcagg ccggctgtca cgtggtgctg gccgaagatg
     5941 gctttgacgc cctggcgaag atcgccgacc acgccccgga cctgatcttc gttgacatca
     6001 tgatgccccg cctggatggc taccagacct gctccctgat caagaagaac gcccgactca
     6061 agtccacgcc ggtgatcatg ctgtcctcca aggacggact gttcgatcgg gcgcgaggcc
     6121 gtctggccgg gtcggatgag tacctcacca agcccttcac caaggaaagc ctgctgcgtt
     6181 ccgtggcgag ccatgtcccg ccccgtgcgg gcgcagccaa ctgatcctca cgccatcgtc
     6241 cgcccatgcc gatcaagaaa atcctcgtcg tagacgactc gcccaccgaa agactggccc
     6301 tgtccgaggt gctcgtgcgc cacggctacc aggtcgtgac cgccgacagc ggcgacgagg
     6361 cagtgatcaa gagcaagacc gaacagcccg acctgatcct gatggacgtg gtaatgcccg
     6421 gcaccaatgg ctaccaggcc acgcgcacca tcgtccgcga tgagcagacc cgcaacatcc
     6481 cggtcatcat ctgcaccagc aagggccttg aaaccgacaa gatctggggc atgcgtcagg
     6541 gcgccttcga ctacctggtc aaacccctcg accacgccat gctgctcgac aagatcaagg
     6601 ccctcggttg acgggatcat ggccaagcgc atcagccttc aggaatttca ggccggcctc
     6661 gcccggcgtc tcgccgaagc ccgcgacacc cgcgcctcgg cccttctcgc cgttcaggcc
     6721 ggttcgcgca actggctgct cgatctggcc gaggctggcg agatcctcgc cgtgccacct
     6781 ctggcgccgg tcccgctggt tcatccctgg ttccgcgggc tcgccagcgt acggggcaac
     6841 cttttcggcg tcgtggactt cgccgccttc cacggcgaag ccccggtggt gaccggcgga
     6901 caggcgcgcc tgctgctcgt cggcgcgcgc ctcaacctca actgcgccct gctcgtctcg
     6961 cgcgccctcg gcctgcgctc ggtggacgat ttcgagcccc tgtccgagcc cgaggacgcc
     7021 accaccccgt ggatcggcgc ccagttgcag gatgcccacg gccacacctg gacacggctg
     7081 cgggtccagg gtctgctgaa tcattcacgc tttcttgacg ctgcactgac atgaattcga
     7141 atcggggagg agcagcgggc atcacccgct gaaaagcaac tatgggattc aagctacctg
     7201 ggctgtcctt tggcagcaaa atggcgacca acaccggcga caacaccatc ctcgaagcca
     7261 gcgagcgcgg cgaggtgagc gcggtgctgc ccttcctggc cggcaagagt acggccgagc
     7321 agttgcgcct gctgcgcctg cccttcattg ccttcgcggt cctgactgcg gtgctggtga
     7381 tctaccagat ccgcgtcagc agcttcggta ccgcctacgt cacggcagcc ggccagatgc
     7441 gcacgctgtc ccagcagatg gccaaggcca gccagctcgc cctgcagggt gaagccggcg
     7501 cctttgccga actcaagcgc agtcgagaac agttcaacca cctgctcgaa gcggtcaccc
     7561 agggcggcga cgtggacggc acgggcgtgc cgggcggcag ttcggcggtc gccgacgagc
     7621 tgaccgcggt caccgagctg tggcagaagt ccgacaagga cgccggccag ctcctcggcc
     7681 aggaaaagaa cctggcgagc ctgggcaatg ccgttgccac catcaacacc aaaaacgccc
     7741 acctcctcga actcaccgaa caggtcgccg ccctcaagct gcagaccggc gcctccgcgc
     7801 gggaaatcgc caccgccaac caggtcgtga tgctgaccca gcggatcgcc aagaacgcca
     7861 acgccctgct cgtcgccaac gcgatcgacc cggaagtggc cttcctcctt ggcaaggaca
     7921 ccaacacctt ccgcgagcag atcgaagccc tgatgaagat gactgccgcc ggctcagacg
     7981 cccagtccaa gctggtggag ctcgaaggcg tggccaagga caacctggcc gccgtgcagg
     8041 ccatcctcgg caacatccag ttgctcgtcc aggccaagca gtccggcagc aacatcttcc
     8101 agaacagcgg cccgttgctg gaaaagacct cggccctcgc cgaaggctac tcccgcgcat
     8161 atacgggctt cttcacctgg gtcaccgtcg gcatcatgct gtttgccgtc ctgtccatcg
     8221 tcctgctgct gctgatggcc aaggtctata acgatgacat tgcccgccgc cgcttctctg
     8281 ccgaacgtct gcgcgagagc gcagagtccg agcgcaacag cacccagcag gccatcctgc
     8341 gcctcatgaa cgagatgggc gacctcgccg acggcgacct caccgtgcgt gccaccgtca
     8401 ccgaagacat caccggcgcc attgccgact cggtgaacta caccattgaa gaactctccg
     8461 tgctggtgcg ccgcatcaac gacgccgcca gccgcgtgac cagcgccacc gaatccgccc
     8521 agcgcacctc cgacgaactg ctcgccgcct ccgaacggca ggcccaggaa ctccgcctcg
     8581 ccggcgatac cgcccacagc atggcggtct ccatgagtga agcctcggaa ggcgccctgc
     8641 agtccgccca ggtggcccgc cgcgccctgg aagccgccca gaaaggggcc accgcggtgg
     8701 aggacaccat caagggcatg aacgacatcc gcgacaagat ccaggacacc tccaagcgca
     8761 tcaagcgcct cggcgaatcg tcccaggaga tcggtgaaat cgtggaactg atctcggaca
     8821 ttaccgaaca gaccaacgtg ctggccctca acgccgccat ccaggccgcc tccgccggtg
     8881 aagcgggccg cggtttctcg gtggtggcgg aagaagtgca gcgcctcgcc gaacgttccg
     8941 ccgaagcgac caagcagatc ggcgccatcg tcaaaaccat ccagaccgac accgccgacg
     9001 ccgtggccgc catggaaaac gccacccgcg acgtggtgga cggcgcctcc ctgtccgaaa
     9061 ccgccggtcg cgaacttgcc gagattgagc ggatctccgc cgaaaccgcc gaactgatcg
     9121 aacgcatctc ggtcagcacc gaaatccagg ccaaatccgc tacccaggtg gccgaaaaga
     9181 tgaagggcat tcttgccgta accgaccgca ccacggcggg cacccagcag actgccgtct
     9241 ccatcggcga actggccgac ctcgccgtgg agctgaaggg ttcggtctcg ggcttcaagg
     9301 tctgaccccg caccgtcacc tggaccggac accgaccgga atgcatcgat gagctccgca
     9361 cacgaactcg accttggccc gctgacctgg gtcaaaggcg aaatcgacct ggccctggaa
     9421 cgcgccgccg gcgccctcgc cgaagccgag acggcctccg accgccttgg ccgcatcaag
     9481 ttcgcccaga cccacctgca ccaggcccac ggtgccctgt ccatcgtcgg tctggatggc
     9541 ctcacccagt tttcggaagc cctcgatcgt ctgctgggcg agctggccgc cgaacaggtc
     9601 acctacacgc cggccatcgc ccgcctcgcc acccggggac tcgccgccct gggcaattac
     9661 ctcaacgaac tgacccgcgg ccagcccgac cagccgctgc gtctctttga actctacgcg
     9721 cagctcgtcg aagcccgcgg gcatgaggca ccgggcccgt cggacctgtt ctaccccgac
     9781 ctctccctgc gtccggcctt cgcaccgctc cccgagggtg gcacggacga tgcgcgcacc
     9841 ctgcgcgccc tgcgcggccg atttgagcgt ggcttgctca aatggctgcg caatcctggc
     9901 gacgccagcg gtcctgccga gatgcgcgat gccgtcaacg ggatcgaacg tactcagcgc
     9961 caacccgcct cgcgcgcatt ctggcttgcc agcgccgcct tcttcgatgc cctcggccag
    10021 caggccatcg acgtcgatcc gcccctcaag cgcctctgcg ggcgcatcga tgcccagatg
    10081 cgacgcctcc tcgaaggctc tgccgtcgtc gcagaacggc tgatgcggga cgtgctctat
    10141 caggtcgcca tcgcccgctg caacacgccc cccgtcaaca ccctgcgcga agcctatcgg
    10201 ctcgtcaacc tgatcccggc ccccggccgg agcgtgagcg acgcaccggt ggcgcccctg
    10261 ctggtggcgc tgcacagcgg gctcggtgat gcgcgtgaag cctggaccct gttcaccggc
    10321 ggcggcgcca tcggcctgcc ccgtttccag gacgccctcg cgcccctccc cgccaccgcc
    10381 cgcgcgattg gccaacccca taccgccgcg ctcatcgaag ggctcggccg cctggcgcag
    10441 tggctgcgcc aggatcccct gcgcctcacc gacaccctgt ccatggacat ggcgacggcc
    10501 atgctgcttg ttgagggcct ggtggacagt cacgaaatgc cgtccccggg tccgcgcctc
    10561 aagaagcaga ttgccgcagt caccgagcgc atccaggcct atctgcgggg cgaacagcca
    10621 gccccgctgt ccatcgccga ccccgacacg gccgatcagc aactgggtcg ccagcttgcc
    10681 catgaaatcc tcgccaacct gggcgacgta gagcagcatc tcgacggatt cttccgggag
    10741 cccgcccgca ccgacctgct ggccgccacc tcggcgcccc tcaaacaggt cgaaggcgca
    10801 ctggcgatca tgaaccaggg cgccgctgta cgcctcgtgc aggactgtgc ggccgccatc
    10861 tccgcgctcg agagcggtcg ccagtcccca gaagcggcca tcttcgaaga catcgcccac
    10921 cgcctctcgg ccctcggcgt gttcatcgac gccctccagc acggaccggc ggatctcgaa
    10981 cagatcctca acccaagtac cggtgacagc gccggcgaca gcctcgaagc cgaactcgtc
    11041 cagcaaaagc gcgaaaccgc cgccctcgcc gccgccctcc aggaaaaccc ggacgacatc
    11101 ggcctgcgca ccgagctgct cctcaacctc gaaggcctgc gccaggatgc cagcctcacc
    11161 gccgacgccg aactcgaaaa gcaggcccgc gaggccctca acctgctcga tgccggcgcc
    11221 cccttcgctg cgccaccggc catcgtgccc gaagcaatgg cgcccgcact gtcgcccgag
    11281 gcggcgcgtc tgctggatgc cagcgacgca gagatcgacg ccgaactgct ggacatcttc
    11341 atcgaggaag ccagcaccgt tcgtgacacc atcgccagcc atctcgacct gctgatcgcc
    11401 gcccccggcg atcaggaaca cctcaccacc atccggcgca gcttccatac cctcaagggc
    11461 agcgggcgca tggtcggcct caaggatttc ggcgaggccg cgtggggcat ggaacagacc
    11521 ctcaaccact ggctgcgcct cgaacgcccg gccacaccgg aactcctggc cctcatccgc
    11581 cgggcccacg tcgcggtgag cgactgggtg gatcagttgc aggcgggcgg cggactgtcc
    11641 ctggacgtgg cggacatcct gcgtgaggca gagcggctgc ggggcgaaga agaccacggc
    11701 gcggcgctcg agatcgtcca gaacctggaa tcgtcgatcc tccagcccat catcgaactc
    11761 gacgcgccgc cccccgcgcc ggccgtcgaa tttcccgcgc tcgattttga ctcgctcgaa
    11821 gcatccggcg agcgcccctc gaccgaccgc ctggacatcg tccttgacga cgcccctgcc
    11881 gagcctcccg tcgcactgga agagctcgtc tgggcagaac cacccgcagt gcccgcgccc
    11941 ccggccccca ccgccgccga acctgcgcca tcggagctcg ccaccgacgc catcgaaatc
    12001 gagattgaaa tcgaagcggc ggcactggaa gacgacgacg tgttcccgga agagggtgcc
    12061 gaggtcatcg atctgggcgc acctgaaccc cttgagcccg acgcgagcga agcggtcgac
    12121 ctgcccgaat ccgacgccac cgcagaggcc atcgaactcg gcgcggccgc gccggagtcc
    12181 gtcgcagacg ccccgtcgca cgggcgggaa cacgacaccg aggatatcga cagcctcgag
    12241 gacgacgatg ccgtgcgcct cggccatgtc gtcctgtcgc gccccctgtt cgacctctac
    12301 ctcgccgaag cccgccagca cctggccacc ctgcgtcacg aactcggccg tatggccgtc
    12361 aatcccagcc tgctgccggc caacgcggcg atccgcgccg cccacaccct gtcgggcatc
    12421 tccggcacgg cccaattcac cgcaccgcaa agcctggcca aggccctcga actcgcccag
    12481 caacgcctcg ccgatcacca ccaggcgccc agcgccgatc aggcccagct gctcaacggg
    12541 gcggccaccg ccctcgagac catgctcgcc cagctttccg ggcgccgact ccccgacgcc
    12601 cagcccgagc tcgtcgacgc tctggacaac gtgctgcgcg gccacctggc cgaagtcatc
    12661 accctgcggc cggaaatacc ctccgacctg ccacccccgg agctcgccac cgcaccgctg
    12721 gcgcgcagcc cggaacccgt cacgccgccg cccgaggcgc cctccgcggc cgctccggtc
    12781 ctgcgtgacg agctcgacgc cgaactgctg cccatcttcc tcgacgaggg taacgaactc
    12841 ctccacagcc tcgacaccgc cctgcgcgcc tggcgcgccc agccggacga tgcgacccag
    12901 ccggccgccg tcgcccggct gctgcacacc ctcaagggca gcgcacggat ggtcggcgcc
    12961 atgagcttcg gcgccagcat ccacgagctg gaaacgcggc tcgagcgcgc ccgtcgcctc
    13021 gccccggacg acatcgcgat cgtcgatgac atcgagtccg cgctcgaccg cagccggcag
    13081 accctcgaaa ccctcgggat cagcccgccc gccacaacgc cggccgccgt ggccgcggcc
    13141 cccatcgatg tcgagacagg cgcagtcgag gcagtgccgg ccggcgccga cggcacgctc
    13201 gcggcccccc gggcccaact gcgggtcgaa gccaccaggg tggaccagtt cgtcaacgag
    13261 gccggcgaaa tcagcatcgc ccgcacccgc atcgaaggcg aactgcgcac cctgcgccgc
    13321 tccctgctgg acctcaccga aaacgtcatc cgcctgcgca accagctgcg cgaagtggaa
    13381 atccaggccg acacccagat gcagtcgcgc ctgtcccagg tcgaaacccg ccataccgaa
    13441 ttcgaccccc tggagatgga ccgctacacc cgcctccagg aactcacccg catgatggcg
    13501 gagagcgtcg gcgacgtgac caccgtgcag cagaatctgc tgcgcaacct ggacggcgcc
    13561 gaaagcgccc tgcatgccca gtcgcgcatg acgcgggacc tgcaacagtc cctgatgcac
    13621 gtgcgcatgg tgcccttcga cagcctcgcc gagcggctct accgcctcgt ccgacagacc
    13681 gccaaggacg tcggccggcg tgccaacctg gacatcatcg gtggccagat cgaaatcgac
    13741 cggggggtgc tggaggccat gaccggccct ctggaccacc tcgtgcgcaa cgccgtggcg
    13801 cacggcatcg aactacccga cgcccggcgc gccgccggca aggccgagat cggccagatc
    13861 accctgcgca tcacccacga aggcaacgaa gtggtgatcg aattccgcga cgacggcgcc
    13921 ggtctggatt tcgatgcgat ccgcgcccac gcgatcgcca ccggcctgct cggcaccgac
    13981 gaagagtccg acgacaagcg cctggccaat ctcatcttcc tgcccggctt cagcaccgcc
    14041 accctgtcca gcgtcgccgg ccgtggcgtg ggcatggatg tggtcaagag cgaaaccgcc
    14101 gccgtcggcg gccgcctcag tgtggaaacc gaccgcggtc gtggtaccac cttccgcatc
    14161 cacctgccgc tcacgctggc cgtcacccag gctctgctgg tgcgttccgg cggccggacc
    14221 tgggcgatcc cctcgtccat ggtcgcccag gtgctggaac tcaaggccgc cgccctcgaa
    14281 aaggtccgcg ccgagcacgg caccgactgg ctcggcgagc gctttgccta tcgatatctg
    14341 ccgcgcctgc tcggcgacag cgccacccag ccccaggttg aacgcttcaa ctgggtgctc
    14401 ctgctgcgcg ccggcagcga aaccctggcg ctgcatgtgg acgccctccg cggcaaccag
    14461 gaagtcgtcg tcaagaacgc cggcccgcag tacgcccgga tggtcggctt ctccggcgcc
    14521 accgtgctcg gcgacggcga gatcgtgctg atcctcaacc cggtggccct cgccgcccgt
    14581 gcgcccgacg ccaccgacga cggcgtaccg ggtctgcccg ccgctccggc gattcccgag
    14641 gtgccgctgg tcatggtggt cgacgactcc ctcaccgtgc gcaagatcac cggccgcctg
    14701 ctggaacggg aaggttaccg ggtgctcacc gccaaggacg gcgtcgacgc gctggagcaa
    14761 ctcggccaca acgacccgga cgtcatcatt gccgacatcg aaatgccgcg catggatggc
    14821 ttcgacctca cccgcaacat ccgcgccgat gcccgccaca agcgggtgcc catcatcatg
    14881 atcacctccc gcatggccga gaagcaccag cgttatgcag aggagatcgg cgtgaaccac
    14941 tacctgggca agccctacga cgaagatctg ctgctcaggc tgatccgccg ctacacccgc
    15001 gcctgacgga ccgtgccgtg ccctcgcccc cgaggggcgg ccgggcttgg ctataatcgg
    15061 tcgttttcct gtccggccgt cccatgaatc tgctcttcga agaggacggt gccttcaaga
    15121 ccggcaccgt cctgaccgac aacgattcca ccctccaggt ggaaactccc catggcaagc
    15181 gcctcaagct caagagcagc cacgtcctcc tgcgcttccc ccagcccgcc gccggcgaac
    15241 tgctggatcg cgccgagatc gaggccgaag gcctcgatac cgaattcctg tgggacgtct
    15301 gcggtgacga cgaattcggt ttcgccgact tcgccaggga atactacggc cacgccccca
    15361 cccccatcga agcgaccgcc gtgctgctgc gcctgcacgc cgcgcccatc tacttccacc
    15421 gcaaggggcg cggccgcttc cgcaaagccc cggccgacat cctccaggcc gccctcgccg
    15481 gcctcgagaa gaagcgccag caggccgccg ccatcgagcg catgcgcgac gcactgctcg
    15541 ccggcaccct gcccgaagag ttccgcccgc tgatccgccc gcttctcttc aagcccgacc
    15601 gcaaccgcct ggagaccaag gcccttgaag cggcctgcgc cgagtgcggc aaatccgccg
    15661 cccgcctgct gttggactgc ggcgccctgg cctcgtccca agaactgcac ttcgaccgtt
    15721 tcctgtacga atacttcccc gaaggcaccg ccttccccag cgtcgacgcg cccaccgcgc
    15781 cggccgacct gccccttgcc ccggtacgcg ccttctccat cgacgacgcc gccaccaccg
    15841 agatcgacga cgccttctcc gtcaccgcca cgcccgatgg ctggcgcatc ggcatccaca
    15901 tcgccgcccc cggcctgggc ttcggccgcg actccgccct cgacgccatc gcccgcacac
    15961 gcctgtccac ggtctacatg cccggtcaca agatcaccat gctgccggac gtcgtcgtgg
    16021 aaggcttcac cctgggtgaa ggccgcgact gcccggccct gtccctgtac ctggagctcg
    16081 cccgcgacct gcgcatcacc ggccaccact cggccctcga acgggtgccc gtcgccgcca
    16141 acctgcgcca ccacgacctg gagccggtgt tcaacgaaac caccctcgcc gacggcggcc
    16201 cggacttccc gtggaaggcc gagctcaccc tgctgtggga gctcgccacc gtcctcgagg
    16261 ccggccgtgg caagcccgcc gccaaccaga ccctggtgga ctacgccttc aacgtcgatt
    16321 ggtccgtcgc cacaccggat ggccccggcc gcgtggacat cggccgccga ccgcgcggct
    16381 cacccctcga caagctggtg gccgaactga tgatctgtgc caacagcacc tggggcaagg
    16441 ccctggctga cgccggcgtt cccgccctgt accgcgccca gaccggcggc aaggtgcgca
    16501 tgaccacctc cgctgcgccc cacgaaggcc tcggcgtcga ctgttacgcc tggtccagtt
    16561 cacccctgcg gcgctacgtg gacctggtca atcaatggca gctcatcgcc tggctgcgcg
    16621 gtgaggcccc cgccttcccg cccaagtcgg ccgacctgat cgccgccatg cgcgacttcg
    16681 aactcaccta cgccgcctac gccgacttcc agcgcggcat ggagcgctac tggtgcgtgc
    16741 gctggctgcg ccagatcgag cgccctgagc tcaccgcccg tgtgttacgg gaaagcctgg
    16801 tgcgcctgga ggacatcccc ctcaccttca agctgccgtc catgcccacc ctgccttccg
    16861 gcacccgtgt gcggctggcc atcgacagca ccgacctgat cgacgtggag gtgcgcgcca
    16921 cctacctgga aaccctcgcc gacgccccgc tcgagatggc cgaggcgctc gccgaagacg
    16981 tcacggacgc gccgctgccc gaagcggccc cgccggcttc ggacgcaccg cccgcgtcct
    17041 gactcccgat gcagcaccct gccccgctcc ctcccgtagc accgccgccc gccccgccct
    17101 cagggatggc ggccgggcgt ctgccgctgg tggccccctc gcgcaaggcg tcccgctatg
    17161 cgctggagtg gggcgtgggc ctgtccctgc tgttgcacgc cctcctgctg tcggttcatt
    17221 tcgtgatgcc cgacgccaac cacttcacgc gcacccatca ggatctcgcc gtggtgctgg
    17281 tcaacgcccg ccacgcccag cgccccgaca aggccgaact cctggcccag accaccctcg
    17341 acgccggcgg caccagcacc cctgacgccc gcgcctcgac gcccctgccg ccccagaccc
    17401 tgagccgcgc cggcgatggc ctcgtcgaag cccagcgccg cgccgaaacg cctcagacgc
    17461 cccagatgca ggccatgacg gcgccgtccg gcaagaccgc ccttgccgtg gacagccgcc
    17521 agaccgcgcc caccgagcct ccccgccagg tctccggcta cgacctcctc gacagctccg
    17581 ccgccgttgc ccgcatcgag gcccagatcg acaaggacct ggtggactac gccgcccgcc
    17641 cgcgcaagaa gttcatcggc gcccgcaccc aggaataccg cttcgcccag tacgtggaag
    17701 actggcgcca gaagatcgag cgcgtcggca ccctcaacta ccccgaagcc gcccgcggca
    17761 aggtgtatgg cagcctgctc ctgtccgtca ccatccgctc ggacggcacc gtggtcagca
    17821 tcgacgtcca tcgctcgtcg gggcacaagg tgctcgacga cgccgcccgg cgcatcgtcc
    17881 agctggccgc gccctacgca atctttccgc cggacatccg ccgcgacacc gacgtgatcg
    17941 agatcacccg cacctggacc ttcaccaact ccgacatgct ccagacctcc gacaagaagg
    18001 aatgaggccc atggatcgct acgccgtcat cggcaacccc atcgcccaca gcaagtcccc
    18061 gcgcatccac gccctgttcg ccgcccagac cgctcaggcc ctgagctatg aggccctgct
    18121 cggccccctg ggcgccttcg ccgccaccgt ggacgccttc cgcgccgccg gcgggcgcgg
    18181 cctcaacgtc accgtgccct tcaagctcga cgccttcgcc tacgccaccc ggcgcacccc
    18241 gcgggccgag gcggccggcg cggtgaacac cctggccttc gggccggacg aggtgctcgg
    18301 cgacaacacc gacggcgccg gtctgctgcg cgacatcacc gtcaacctgg gctgtcccct
    18361 gacggagcgg cgcgtgctcc tgctcggcgc gggcggcgcc gcccgcggca gcctgctgcc
    18421 cctgctgctg accgaaccgg cccgcctcgt cgtcgccaac cgcagcagcg ccaaggccgt
    18481 cgcactggcc gccgaattcg cccgcctgcc gtcctgtgcc gtggccgtca agctcgaagg
    18541 ctgcggcttt gccgacctgg ccggcgccca gttcgacgtc gtcatcaacg ccaccagcgc
    18601 cagcctcggc gacgccgcgc ctgatctgcc cgcgggcctc tacgcccccg gcagcctcgc
    18661 ctacgacatg atgtacggca agggcgacac ccccttcctg gccgccgccc gcgcccaggg
    18721 cgccgggcgc ctcgccgacg gcctgggcat gctggtggag caggccgccg agagcttcgc
    18781 cctgtggcgt ggcgtgcgcc ccgacaccgc accggtgctg gccgccctgc gcgccggctg
    18841 agcccgcgac ccggcaccgc cccatgaaga ccttcgggcg ctggctcaaa cgcggactgc
    18901 tgggcctggt gctggttttc ctggcctggc acctgtggct gctcggccac gtcctgtggt
    18961 ggaagtggca gcccccgtcc gagaccagct tcatgagcct gcgcctggac gaacttgcca
    19021 cccgccggcc cgacgccgaa ctgcgctacc gctgggtgcc ctacgagcgc atctccgtct
    19081 acctcaagcg tgccgtggtg gccgccgagg acgacaagtt caccgatcac gaaggcttcg
    19141 actgggacgg catccagaag gccatcgaga agaaccagaa gaagggccgt gccgtggccg
    19201 gcggctccac catcagccag cagctcgcca agaacctctt cctgtccccc gcccgcagct
    19261 acctgcgcaa ggccgaggag gccctcatca cggtgatgat cgaaaccttc tgggacaagc
    19321 gccgcatcct ggaggtctac ctcaacgtcg tcgaatgggg tgacggcatc ttcggctgcg
    19381 aggccgcggc ccagcgctat ttccacatct ccgccgccca gctcggcccc gcccaggccg
    19441 cccgcctggc ggtgatgctg cccaacccgc gcaagtacga gaaggacttc ggcccgcgcc
    19501 tggccgccca tgcagcgcgc atccaggcgc gcatgcagta ctccgacgtg ccgtgacagg
    19561 cgacgcacgc aggcgcatcg ccccctgccc ggccggcgcc cccatggggt agaatgcgat
    19621 gctttccccc tgcagtgcct ccccgatgca attcgccggt ttcaccttgc gaaacaatct
    19681 cttcgtcgcg cccatggcgg gcgtgacgga ccgtcctttc cgccagcttt gcaaaaagat
    19741 gggggcgggt ctggccgtct ccgagatggt cacctccaac tccctgctct acggcagcgc
    19801 caagacccag cgccgcgcca accacgacgg tgaggtcgac ccgatctcgg tgcagatcgc
    19861 cggcgccgat ccggcgatga tggccgaggc cgcccgccac aacatcgacc gcggcgccca
    19921 gatcatcgac atcaacatgg gctgcccggc caagaaggt
//
LOCUS       DAOQPA010000062        19622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1380_length_19622_cov_20.150560, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000062 DAOQPA010000000
VERSION     DAOQPA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19622
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_19622_cov_20.150560"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PKX01_09990"
     CDS             <1..1376
                     /locus_tag="PKX01_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HMZ76499.1"
                     /translation="TVDNAAFDAAMARQKEQARAAGKFKMAANLEYDGPATTFHGYDS
                     LEHGGNILALYKDGAAVNELHEGDLGVVVLDDTPFYAESGGQVGDRGELRSVHGIFAV
                     EDTQKIQASVFGHHGVVKTGTLSVGNGVAARVDLQARTRTMRNHSATHLMHKALRQVL
                     GEHVQQKGSQVDPDKTRFDFVHTQPMSNEQIRQVENIVNAEILANTPSETRVLPIEEA
                     QKLGAMMLFGEKYGDSVRVLDIGFSRELCGGTHVQRTGDIGLFSIVAEGGVAAGVRRV
                     EAVTGDNALRYVQGMETALGGVAGTLKVLRSEVPARVNALLDQTRRLERELAALKGKL
                     ASAQGDDLGSRAVEVKGVKVLAASLDGADSVTLRETLDKLKDKLKSAIIVLATVADGK
                     VSLIAGVTADLTGKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVTD
                     WVGAKLG"
     gene            1381..1932
                     /locus_tag="PKX01_09995"
     CDS             1381..1932
                     /locus_tag="PKX01_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ76500.1"
                     /translation="MTPQNWRFCPQCGAPLEWAAVAGAERQACYAPGCGFVRWDNPVP
                     VVAAVIECVDRDGAVLLARNHVWPERMFALVTGFLERGESPEEAVAREVREETALEAE
                     SVRLLGVYPFERKNEVIIGFHVRARGEIVLNEELAEFRLVPPARLRPWPRATGLFLRD
                     WLLVQGLPVPELVEDNLFAHADL"
     gene            complement(2023..3405)
                     /locus_tag="PKX01_10000"
     CDS             complement(2023..3405)
                     /locus_tag="PKX01_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HMZ76501.1"
                     /translation="MFVPAPEIFKAYDIRGIVDKTLTAEAVRAIGHALGSEAVARGQK
                     AIAVGRDGRLSGPELAGALADGIRAAGVDVVDVGCVPTPLTYFAAYELGTHSCVSVTG
                     SHNPPDYNGLKMVLGGQTLFGDLIQSLRQRIIDGNLVTAAEPGRLTHADVVPAYIAKI
                     VGDVKLSRPMKIVMDCGNGVAGAVAPELFKRLGCELVELFCEVDGHFPNHHPDPSKPE
                     NLEDVIRALRDTDAEIGLAFDGDGDRLGVVTKDGEIIFPDRQLMLFAEDVLTRVPGGE
                     IIYDVKCTRNLAGWIQARGGKPTMWNTGHALVKAKLKETGAPLAGEMSGHVFFKERWY
                     GFDDGLYTGARLLEIVSRHADANPVLKGLPNATSTPELNLKMAEGEPFALVDKLKAEA
                     RFEGAESILTIDGVRVEYADGFGLARPSNTTPVVVLRFEADNEAAIARIQADFRRAIE
                     GVWPGVQLPF"
     gene            complement(3441..4442)
                     /locus_tag="PKX01_10005"
     CDS             complement(3441..4442)
                     /locus_tag="PKX01_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HMZ76502.1"
                     /translation="MTIPPYRAPRWLPGGHAQTIWPLRCTGPLPNFQRERWTTPDGDF
                     IDLDWLPLQPAAPLVVLFHGLEGSSHSPYARALMGHLASLGWNGVVPHFRGCSGEPNR
                     APRAYHSGDADEIEWILTRMAERFPDRPRYAAGVSLGGNALLLWLGTRGTEARRTIDA
                     AAAICAPLDLAAAGHHLARGFNRVYTRHFLSTLKRSAEAKLDRFPGLFDRAAMLRAET
                     LHAFDNVVTAPLHGFRDTDDYWRRASSKPHLRHIAVPTLVLNARNDPFLPAHHLPDVS
                     QVSSAVHLEHPEEGGHAGFVRGPWPGRLDWLPARLVHFFTNLNGSFHPLKSVMLHCV"
     gene            complement(4439..4870)
                     /locus_tag="PKX01_10010"
     CDS             complement(4439..4870)
                     /locus_tag="PKX01_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ76503.1"
                     /translation="MSSILYTSPDDAEAAFYDAMGRADLNAMMTIWSEDEEVVCIHPG
                     SIRLVGLGAIRESWRQLFASGMRLHVRPTHLIRWHNLMTAVHTVHQRVHVEGDDRLHP
                     PIIATNVYTRGASGWRMVMHHTSSSPDVDNLQGHDGPHVVH"
     gene            complement(4962..5162)
                     /locus_tag="PKX01_10015"
     CDS             complement(4962..5162)
                     /locus_tag="PKX01_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HMZ76504.1"
                     /translation="MPENQDKAQAIEVTAKNLPLHCPMPDAPLWARHPRVFLDVAGEG
                     HATCPYCGAKYVFKGELPKGHH"
     gene            complement(5182..6102)
                     /locus_tag="PKX01_10020"
     CDS             complement(5182..6102)
                     /locus_tag="PKX01_10020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HMZ76505.1"
                     /translation="MSMADRDGFIWYDGKLVPWREATTHVLTHSLHYGLSVFEGVRAY
                     NTVKGTAIFRLAEHTQRLLNSGKIYMMNIPYSKEELMEAQLEVVRVNKLESCYLRPIA
                     FYGSEKMGISTKGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSFARHHVNVTMPRAK
                     LASTYANSILANLEATEAGYDEALLLDTNGFVAEGAGENLFVIKDGAIYEPEIASALT
                     GITRASVIQVAKEFGLDVISKRITRDDIYIADEAFFTGTAAEVTPIRELDGRVIGEGK
                     RGPVTQKLQARFFDIVNGRAPEYAHWLSFI"
     gene            6229..6681
                     /locus_tag="PKX01_10025"
     CDS             6229..6681
                     /locus_tag="PKX01_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ76506.1"
                     /translation="MDIWKPNVTVAAVVERDGRFLLVEEETDAGVRFNQPAGHLDQGE
                     SLVTAAAREALEETAHHFVPEFLVGIYQWPRPAGDITYLRFAFGGQVTGFEPERALDS
                     GILRAVWMTLDEVRATRERHRSPLILQCCEDYRAGRRYPLDLIRHYDA"
     gene            6681..7232
                     /locus_tag="PKX01_10030"
     CDS             6681..7232
                     /locus_tag="PKX01_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76507.1"
                     /translation="MRALGWLVLLALAGSTWADESVSICYDYGCAAEARVVYSEPRLV
                     EIRARLALADNAVAERAVLAQVVGQLYRWAGEQSPIHADRAGDFLDDGVDGKMDCIDH
                     AESTTRLLHMLEVRGMLRFHDVDQIRRRTRWLVTQHFSAVIRARDEGAARFVVDSWFG
                     EHGDPAVILPLEEWLDGEGPNVS"
     gene            7222..8334
                     /gene="mnmA"
                     /locus_tag="PKX01_10035"
     CDS             7222..8334
                     /gene="mnmA"
                     /locus_tag="PKX01_10035"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMZ76508.1"
                     /translation="MSRKEKVIVGMSGGVDSSVAALLLKQQGYDVVGLFMKNWEDDDD
                     DEYCSTRQDLVDVASVADVIGIDLEVVNFSKEYKDRVFADFLREYEAGRTPNPDILCN
                     SEIKFRAFLDHALALGADRIATGHYAQVREWTNDGRTEFQLLKAEDGTKDQSYFLYRL
                     NQAQLSKTLFPIGHLYKREVRRMAEAAGLHVFDKKDSTGICFIGERPFREFLSRYLPA
                     RPGEIRTLEGDKVIGQHQGLMYHTLGQRKGLQIGGIKGNQDEAGEHDAWYVARKDVAK
                     NVLYVVQGHDHPALLRDRLAAAELSWVSGRDPRTHWVYTAKPRYRTPDAPCEIDALAD
                     GRAEIAFAQPQWAVTPGQSVVIYESRVCLGGGIIVE"
     gene            complement(8425..9054)
                     /locus_tag="PKX01_10040"
     CDS             complement(8425..9054)
                     /locus_tag="PKX01_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ76509.1"
                     /translation="MRHLSPRLLRCAAALALAGGCFGAQAGNQIYEPLAASVRAALHA
                     AVSDQAPPDTRFTRSPERARWLDEMSRRLARRMPDEAGRLEFLRAVHYEATRAGLDPQ
                     LVLGLIQVESGFKKYALSTAGARGYMQVMPFWVDLIGTSDSNLFHMRTNLRFGCTILR
                     HYLDIERGDLFRALGRYNGSLGRAEYPNLVRGAWENNWSYALFRVAQNP"
     gene            complement(9057..10775)
                     /locus_tag="PKX01_10045"
     CDS             complement(9057..10775)
                     /locus_tag="PKX01_10045"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMZ76510.1"
                     /translation="MRASRFFLSTLKEAPSDAEIVSHKLMLRAGLIKRLAGGIYTWMP
                     LGLRALRKVEAIVREEMNRAGAIEVLMPAVQPAELWEESGRWDFYGPELLRFKDRHDR
                     AFVIGPTHEEVITDVARRELRSYRQLPKHFYQIQMKFRDEIRPRFGVMRGREFVMKDG
                     YSFHTSFEDLQREYRNMYDTYHRIFARLGLKFRAVAADTGSIGGTGSHEFHVIAETGE
                     DDIAYCPDSDYAANVELAEALAPATPRPVPAAPLTKVSTPGVKSIEDVAAFLKVPVDT
                     TVKAVVVQGDDSVGGRPVLLLLRGDHELNEVKAQKLAGVRNPLTFATPESIREAFGAA
                     PGSLGPVGFGGRVVMDRTVAAMADFVTGANDDDHHFTGVNIGRDFPEAEIADLRNVVA
                     GDPSPDGLGTLEICRGIEVGHIFQLRTKYSEALGCKFLNEQGKEQVMEMGCYGIGVSR
                     ILGAAIEQNNDARGIIWPDAIAPFQVAIVPMGYAKSDAVRAAADTLYADLSAAGIDVF
                     LDDRDERPGSLLADNELIGTPHRVVIGDRGLKDGVVEYQGRRDAEATRWPVAEASANL
                     LARLKA"
     gene            10930..11490
                     /locus_tag="PKX01_10050"
     CDS             10930..11490
                     /locus_tag="PKX01_10050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HMZ76511.1"
                     /translation="MLDREGYRPNVGIILVNARNEVFWGKRIREHSWQFPQGGIKHGE
                     SPEQAMYRELYEEVGLKPEHVRIIGRTRGWLRYDVPKHWIKREWRNTYRGQKQIWYLL
                     RLVGRDSDVSLRASNHPEFDAWRWSSYWVPLEAVIEFKRGVYQSALLELARLLFRDRF
                     FNIPDSYLPHPAEPLEGEGADAGPRR"
     gene            11664..12257
                     /locus_tag="PKX01_10055"
     CDS             11664..12257
                     /locus_tag="PKX01_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ76512.1"
                     /translation="MERMHYQPISQFKVSRGTCAVAEAWGLTRVTASSPAVEVMTDLR
                     RIPAATIGQDVSLAEANHAMILRGVRLLFVTDAARRVVGIVTATDLLGERPTQVARER
                     SLRYEELTLNEIMVPSEALDAVSMSDVLRAEVGHVVATLKSSGRQHALVVEETPEGQP
                     TIRGIFSASQIARQMGIPLQTTEVARTFAEIEAAIAH"
     gene            12281..12874
                     /locus_tag="PKX01_10060"
     CDS             12281..12874
                     /locus_tag="PKX01_10060"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HMZ76513.1"
                     /translation="MNHGVHVAGARLAGRSGRLASLFFGLCAGLLASVGAVAQVAQQS
                     LFTSPEPPAWAESPVEIPKELPDDAALAPFSVSRAATATFLIDTSSVTRGEDGIWRFA
                     LVIRTAGGAENITYEGIRCATAERRLYAIGRRGEGWTASRNDAWGRIAESSLNRYHAA
                     LFKDYFCPPGLAQPSPAEAVSRLRSGMPILPTGNLTP"
     gene            12871..13491
                     /gene="coq7"
                     /locus_tag="PKX01_10065"
     CDS             12871..13491
                     /gene="coq7"
                     /locus_tag="PKX01_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HMZ76514.1"
                     /translation="MIDQLILGFDRALQTVFASASTVRPTPGEDLPETELSDAERRHA
                     AALMRINHCGEICAQALYQGQALMSDNDGIRRALESAAQEETEHLAWTERRIAELGGR
                     KSLLNPLWYGGSLAIGMAAAKIGDGFNLGFLAETERQVEAHLKSHLDDLPQKDGRTRA
                     IIEQMKTDEVAHAETAVRLGAVPLPPPVRGAMKLMSRVMTRTAYWL"
     gene            complement(13546..13962)
                     /locus_tag="PKX01_10070"
     CDS             complement(13546..13962)
                     /locus_tag="PKX01_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ76515.1"
                     /translation="MECVIKWVDGMSFLAETGSGHTVMMDGAPEAGGRNIAPRPMELM
                     LAGAGGCTAFDVVLILKRSRQDVTDCKVSLKADRAETDPKVFTRIAFHYTVTGRNLKP
                     DVVERAIKLSYEKYCSATIMLGKTAEIEHTWDIVEA"
     gene            14468..14896
                     /gene="rplM"
                     /locus_tag="PKX01_10075"
     CDS             14468..14896
                     /gene="rplM"
                     /locus_tag="PKX01_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMZ76516.1"
                     /translation="MKTFSAKPHEVKRDWFVVDATDKVLGRLASEVARRLRGKHKPEY
                     TPHVDTGDYIVVVNVDKLRVTGNKALDKKYYRHSGYPGGIYETNFTKLQQRFPARVLE
                     KAVKGMLPKGPLGYAMLKKMKCYAGGEHPHAAQQPKVLEI"
     gene            14906..15301
                     /gene="rpsI"
                     /locus_tag="PKX01_10080"
     CDS             14906..15301
                     /gene="rpsI"
                     /locus_tag="PKX01_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMZ76517.1"
                     /translation="MMAVTYNYGTGRRKSAVARVFIKKGTGNIVVNGKPVDQFFSRET
                     GRMIVRQPLVLTNFADSFDILVNVTGGGESGQAGAVRHGITRALIDYDAELKSPLKKA
                     GLVTRDAREVERKKVGFRKARRRKQFSKR"
     gene            15433..16461
                     /gene="argC"
                     /locus_tag="PKX01_10085"
     CDS             15433..16461
                     /gene="argC"
                     /locus_tag="PKX01_10085"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HMZ76518.1"
                     /translation="MIKVGVVGGTGYTGVELLRLLAQHPEAEIRAITSRGEAGMPVAD
                     LFPSLRKRVDLCFVDPQAADLASCDVVFFATPNGIAMQQAAALVGAGVRVIDLAADFR
                     IKDVAEWEKWYGMKHACPELVAEAVYGLPEVNREAVRQARVVANPGCYPTAVQLGFLP
                     LVEAGVIELDHLVADAKSGVSGAGRKAETHILFSEAADNFQAYGVAGHRHLPEIRQGL
                     AGFAGQAVGLTFVPHLTPMIRGIHATLYGKLKKDVDLQALFEERFAGEPFVDVLPKGS
                     HPSTRSVRSANMCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNMNILFGLPETTG
                     LGQVPVMP"
     gene            16561..16911
                     /gene="erpA"
                     /locus_tag="PKX01_10090"
     CDS             16561..16911
                     /gene="erpA"
                     /locus_tag="PKX01_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HMZ76519.1"
                     /translation="MNAVTEMPAPLVFTDSAVGKVKDLIAEEGNPELKLRVFVTGGGC
                     SGFQYGFTFDEIRNDDDTVMEKDGVMLLIDPMSYQYLVGAEIDYTEGLEGSQFVIRNP
                     NATSTCGCGSSFSA"
     gene            complement(16979..18301)
                     /locus_tag="PKX01_10095"
     CDS             complement(16979..18301)
                     /locus_tag="PKX01_10095"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ76520.1"
                     /translation="MLREKTGILAHLKLLRDGDRKALLKSMALGAGVFTGMAAALAVA
                     PGQDAPPPAETIVTQLERPAFDISGRDEHPFIREDRVLAGETLGALLKRLGVSDTSLT
                     AHAGSPALMRDLGTAFRPGMVVTVATTTDGRLQSASFLRPGNDDQVVLTAENGQLVIR
                     KQALELDRRIHMQSGTIQSSLFAATDAAGLPDSIAEELARMFGSEIDFHSDLRKGDRF
                     SVIYEVFYHHGQAIRTGRILAAEFFNQGVKHSAYLFRHADGKTEYYGPDGRSLRAGFL
                     RSPLEFSRVTSGFSMRLHPILGTWREHKGVDYGAPTGTAVHATADGIVDFAGWQNGYG
                     NFVVLRHQDRYSTAYGHLSAVARGLRKGDRVSQNTVIGYVGSTGWATGPHLHYEFRIN
                     EIHQDPLKVSLPAAQPLDRQKLGSFLHDVAQLNAQLTAARQITTARFD"
     gene            18375..19574
                     /gene="tyrS"
                     /locus_tag="PKX01_10100"
     CDS             18375..19574
                     /gene="tyrS"
                     /locus_tag="PKX01_10100"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMZ76521.1"
                     /translation="MTEITQALELIKRGSDELLVESELVDKLKSGRPLRVKAGFDPTA
                     PDLHLGHTVLINKLRHFQDLGHQVLFLIGDFTGMIGDPSGKNATRPPLSREQVLANAQ
                     TYQEQVFKILDPARTEVVFNSTWMESLGASGMIRLAAQHTVARMLERDDFSKRFGNQQ
                     PIAIHEFLYPLCQGYDSVALKADVELGGTDQRFNLLMGRELQKNAGQSPQCVLTMPLL
                     EGLDGVNKMSKSLGNYIGIYEPPGEIFGKIMSVSDDLMWRYYDLLSFRSSAEVAGLKE
                     AVQTGRNPRDVKVALAQEIVARFHGEPAAEAALTDFEARFQRNAIPDDLPEVAISGGE
                     TGLSVVAVLKQAGLTGSTSEAMRMIDQGGVRLNGEKVSDKGLVLKAGAVVVLQVGKRK
                     FARVTLR"
BASE COUNT         3342 a         5792 c         6895 g         3593 t
ORIGIN      
        1 tgaccgtcga caacgccgcc ttcgacgccg ccatggcgcg ccagaaggag caggcgcgcg
       61 ccgccggcaa attcaagatg gccgccaatc tggagtacga cggcccggcg accaccttcc
      121 acggctacga cagcctggag cacggtggca acatcctggc cctctacaag gacggtgccg
      181 cggtcaatga actccacgaa ggcgacctgg gtgtcgtcgt gctggacgac accccgttct
      241 acgccgaatc cggcggccag gtgggcgacc ggggcgagct gcggtcggtg cacggcatct
      301 tcgccgtgga ggacacccag aagatccagg ccagcgtgtt cggccaccac ggcgtggtca
      361 agaccggcac gctcagcgtt ggcaacggcg ttgcggcccg tgtcgatctg caggcccgca
      421 cccgcaccat gcgcaaccac tccgccaccc acctgatgca caaggccctg cgccaggtgc
      481 tgggggagca tgtgcagcag aagggctccc aggtggatcc ggacaagacg cgcttcgact
      541 tcgtccacac ccagcccatg agcaacgagc agatccgcca ggtcgagaac atcgtcaatg
      601 ctgagatcct ggccaatacg ccctctgaga cccgcgtgct gcccatcgaa gaggcccaga
      661 agctgggcgc catgatgctg tttggcgaga agtacgggga ttccgtgcgc gtgctcgaca
      721 tcggcttctc ccgcgaactg tgcggcggca cccacgtaca gcgcaccggc gacatcggct
      781 tgttcagcat cgttgccgaa gggggcgtgg cggcaggtgt gcgccgcgtc gaggcggtga
      841 ccggtgacaa cgcgctgcgc tatgtgcagg gcatggaaac cgcgctgggc ggggtggccg
      901 ggacgctcaa ggtactgcgc agcgaggtgc cggcgcgggt caatgccctg ctcgaccaga
      961 cccgccgtct ggagcgcgaa ctggccgccc tcaagggcaa gctggcgtcg gcccagggcg
     1021 acgatctggg cagccgcgcc gtggaggtga agggcgtgaa ggtgctggcg gcgtccctgg
     1081 acggtgccga ttcggtcacg ctgcgggaga ccctcgacaa gctcaaggac aagctgaagt
     1141 ccgcgatcat cgtgctggcc acggtggccg acgggaaagt cagcctgatc gccggcgtca
     1201 cggccgatct gaccgggaag gtcaaagccg gcgagctggt caacttcgtc gcccagcagg
     1261 tcggcggcaa gggtggcggg cgtccggaca tggctcaggc gggcggaacg gacgcagcag
     1321 cgctgccggc ggcgctggcg tccgtgaccg attgggtcgg cgccaagctg ggctgaatcc
     1381 atgacgccac agaactggcg cttctgcccc cagtgcggcg cgcccctcga atgggcggcc
     1441 gtggcagggg ccgagcgcca ggcctgctac gcgccgggct gtggcttcgt ccgctgggac
     1501 aatccggtgc cggtggtggc cgcggtgatc gagtgcgtgg atcgggatgg tgccgtcctg
     1561 ctggcgcgca atcacgtgtg gcccgagcgc atgttcgcgc tggtgaccgg ctttctggag
     1621 cggggtgaaa gtcccgagga ggcggtggcg cgggaggtgc gggaggagac ggcgctggag
     1681 gccgagtccg tgcgtctgct gggcgtctat cccttcgagc gcaagaacga ggtgatcatc
     1741 ggttttcacg tgcgggcacg gggcgagatc gtcctcaacg aagagctggc ggaattccgc
     1801 ctggtgccgc cggcgcgcct gcgcccatgg ccgcgggcaa ccggcctgtt cctccgcgac
     1861 tggctgctgg tgcagggcct gcctgtgccg gaactggtgg aggacaacct gttcgcccac
     1921 gccgatctgt agcgtgcctc cggccggcac cgccaacaac aaaggccacc ccgcggggtg
     1981 gccttttgac tgctgccggt cgcggaccgg catgggcgat gcttagaacg gcaactgtac
     2041 tcccggccac acgccttcga tggcgcggcg gaaatcggcc tggatgcgcg cgatggcggc
     2101 ctcgttgtcg gcctcgaagc gcagcacgac caccggggtg gtgttggacg gacgggccag
     2161 gccgaaaccg tcggcgtatt ccacgcgcac gccgtcgatg gtgaggatgc tctcggcgcc
     2221 ttcaaaacgg gcttcggcct tgagcttgtc caccagcgcg aagggctcgc cttcggccat
     2281 cttgaggttg agttccgggg tgctggtggc gttgggcagc cccttcagca ccgggttggc
     2341 gtcggcgtgg cgggaaacga tttccagcag gcgggcgccg gtgtagaggc cgtcgtcgaa
     2401 gccgtaccag cgttccttga agaatacgtg gccgctcatc tcgccggcca gcggggcacc
     2461 ggtttccttg agcttggcct tgaccagggc gtggccggtg ttccacatgg tgggcttgcc
     2521 gccgcgggcc tggatccagc cggccagatt gcgggtgcat ttgacgtcgt aaatgatctc
     2581 gccaccgggc acgcgggtga gcacgtcttc ggcgaagagc atgagctggc gatcggggaa
     2641 gatgatctcg ccgtccttgg tgaccacgcc cagtcggtcg ccgtcgccgt cgaaggcgag
     2701 gccgatctcg gcgtcggtgt cgcgcagggc gcggatcacg tcctccaggt tctcgggctt
     2761 ggaggggtcc gggtgatggt tggggaagtg gccgtccacc tcgcagaaga gctccacgag
     2821 ttcgcagccc agacgcttga agagttccgg cgccaccgca ccggccacac cgttaccgca
     2881 gtccatgacg atcttcatcg ggcgcgacag cttgacgtcg ccgacgatct tggcgatgta
     2941 ggcgggcacc acgtcggcgt gggtcaggcg gccgggttcg gcggcggtga ccaggttgcc
     3001 gtcgatgatg cgctggcgca ggctctggat caggtccccg aacagggtct gaccgccgag
     3061 gaccatcttg aggccattgt agtccggcgg attgtggctg ccggtgacgg acacgcagga
     3121 gtgggtgccc agttcgtagg cggcaaagta ggtgaggggc gtgggcacgc agccgacgtc
     3181 caccacatcc acgccggcgg cgcggatgcc gtcggcgagg gcgccggcca gctcggggcc
     3241 ggacaggcgg ccgtcgcgcc ccacggcgat ggccttctgg ccgcgggcga cggcttcgga
     3301 cccgagggcg tggccgatgg cgcggacggc ttcggcggtc agggtcttgt ccacgatgcc
     3361 gcggatgtca taggccttga agatctcggg ggcggggacg aacatgctcg aaactccagt
     3421 gttaaaagat gaaaaccgca ttatacgcag tgcagcatga cgcttttcag cggatgaaag
     3481 gatccattca ggttcgtgaa aaaatgcacg aggcgggcgg gcagccagtc cagacgtccg
     3541 ggccaggggc cgcgcacgaa acccgcatga ccgccttcct cggggtgttc cagatggacg
     3601 gccgacgaga cctgtgaaac gtcgggcagg tggtgggccg gcagaaaggg gtcattgcgg
     3661 gcgttgagga ccagggttgg cactgcgatg tggcgcaggt ggggcttgct gctggcacgc
     3721 cgccagtaat cgtcggtgtc gcgaaagccg tgcagcggcg cagtgacgac gttgtcgaat
     3781 gcgtggagcg tttcggcccg gagcatggcg gcccggtcga agaggccggg gaagcggtcg
     3841 agcttggcct cggcgctgcg tttgagggtg ctcaggaagt ggcgcgtgta gacccggttg
     3901 aagccgcggg cgaggtggtg acccgcggcg gccagatcca gcggcgcgca gattgctgcg
     3961 gcagcgtcga tggtgcggcg ggcctcggtg ccgcgggtgc cgagccacag gagcagggca
     4021 ttgccaccga gggacacgcc ggccgcgtag cggggccggt cggggaaacg ctccgccatg
     4081 cgtgtcagga tccactcgat ctcgtccgcg tcacccgaat ggtaggcgcg cggcgcccga
     4141 ttgggttcgc cggagcagcc gcggaagtgg gggaccacgc cgttccagcc gaggctggcc
     4201 aggtggccca tcagggcgcg ggcgtagggg gagtggctgg agccttcgag gccgtgaaag
     4261 agcacgacca gcggggcggc gggctggagg ggcagccagt ccaggtcgat gaagtcgccg
     4321 tccggcgtgg tccagcgctc acgctggaaa ttcgggaggg ggcccgtgca gcgcagcggc
     4381 cagatcgtct gggcatgccc gccgggcagc cagcgcggcg cgcggtaggg cgggatggtc
     4441 aatggaccac gtgggggccg tcgtggcctt gcagattgtc cacgtcgggg gaggacgagg
     4501 tgtggtgcat gaccatgcgc cagccactgg cgccgcgggt atagacgttg gtggcaatga
     4561 tgggcgggtg gagacggtcg tccccttcca catgcacacg ttggtgcacg gtgtgcaccg
     4621 cggtcatcag gttgtgccag cggatcaggt gggtcggccg gacgtggagg cgcatgccgc
     4681 tggcgaagag ctggcgccag gattcgcgga tggcaccgag gcccaccaga cggatgctgc
     4741 cggggtgaat gcagacgact tcctcgtcct cggaccagat ggtcatcatg gcgttgaggt
     4801 cggcccgacc catggcgtca tagaaggcgg cctcggcatc gtccggggag gtgtacagga
     4861 tggagctcat ggggataggc aagacggcgc tgcggtgagg tccgcacgcg tgctgcggga
     4921 gcccggaggc ccccgcctgg tgaggcttgg cccggggcgg gtcagtgatg acccttgggc
     4981 aattcaccct tgaagacata cttggcacca cagtaggggc aggtggcatg cccttcgccc
     5041 gcgacgtcga ggaagacccg cggatgacgg gcccacaggg gggcatccgg catcgggcag
     5101 tgcagcggca ggttcttggc ggtgacttcg atggcttgcg ccttgtcctg gttttcaggc
     5161 atgagggttc tcccggggat ctcagatgaa tgacagccag tgtgcgtatt cgggggcgcg
     5221 accgttcacg atgtcgaaga agcgggcctg gagcttctgg gtgacggggc cccgcttgcc
     5281 ttcgccgatg acccggccgt ccagttcgcg gatcggcgtg acctcggcgg cggtgccggt
     5341 gaagaaggct tcgtcggcaa tgtagatgtc gtcgcgggtg atgcgcttgg agatgacgtc
     5401 caggccgaat tccttggcaa cctggatgac cgaggcgcgg gtgatgccgg tgagggcgga
     5461 ggcgatctcg ggttcgtaga tggcgccgtc cttgatcacg aagaggttct cgccagcgcc
     5521 ttcggccacg aagccgttgg tgtcgaggag cagggcttcg tcgtaaccgg cttcggtggc
     5581 ttccaggttg gccaggatgg agttggcgta ggtggaggcc agcttggcgc ggggcatggt
     5641 gacattgacg tggtgacgcg cgaaggacga ggttttgacg cggatgccct tctcgaggcc
     5701 gtcttcaccc aggtaggcac cccagggcca ggcggcgatg gccacatgca cggtggcgcc
     5761 cttggtggag atgcccatct tctcggagcc gtagaaggcg atggggcgca ggtagcagga
     5821 ttccagcttg ttgacgcgca cgacttccag ctgggcttcc atgagttctt ccttgctgta
     5881 aggaatgttc atcatgtaga tcttgccgga gttgagcagg cgctgggtgt gctcggcgag
     5941 gcggaagatg gcggtgccct tgacggtgtt gtacgcgcgg acgccctcga agacggaaag
     6001 accgtagtgg agggagtggg tgagcacgtg ggtggtcgct tcacgccacg gcacgagctt
     6061 gccgtcgtac cagatgaaac cgtcgcggtc tgccattgac attatgggga tctccagcga
     6121 aatgcattga attcgagcct tcgattctag cgcattccgc cggcctgcgg tccgctcatc
     6181 ggcgcggggt agaatgtgca tttttccgta cggatcgcga tgaagccgat ggatatctgg
     6241 aaacccaatg tcacggtggc cgccgtggtg gaacgggacg gccgcttcct gctggtggag
     6301 gaggaaaccg acgccggggt gcgcttcaac cagccggccg ggcatcttga ccagggggag
     6361 tccctggtga cggccgccgc acgcgaggcc ctcgaggaaa ccgcccacca tttcgtaccc
     6421 gagtttctgg tgggcatcta tcagtggccg cggccggcgg gtgacatcac ctacctgcgc
     6481 tttgcctttg gtggccaggt gaccggcttc gagccggagc gggccctcga ttcggggatc
     6541 ctgcgggccg tgtggatgac cctcgacgag gtacgcgcca cccgcgagcg ccatcgcagc
     6601 cccttgatcc tgcagtgttg tgaggactat cgcgcggggc ggcgatatcc gctcgacctg
     6661 atccgccact acgacgcctg atgcgtgccc tggggtggct ggtgttgctg gcgcttgccg
     6721 gctccacctg ggcggatgag tcggtgtcga tctgttacga ctatgggtgc gccgcggagg
     6781 cccgggtggt gtattcggag cctcgcctcg tcgagatccg cgcacgcctc gccctggccg
     6841 acaacgcggt ggccgagcgg gcggtgctgg cacaggtggt ggggcagctt taccgttggg
     6901 cgggcgagca gtcgccgatc catgcggatc gtgcgggaga cttcctcgat gatggtgtgg
     6961 acggcaagat ggactgcatc gatcatgcgg aatccaccac ccgcctgctg catatgcttg
     7021 aagtccgcgg catgctgcgt ttccacgacg tggaccagat ccgccggcgc acgcgctggt
     7081 tggtgaccca gcatttttct gccgtgatcc gtgcgcggga cgagggtgcc gcgcggtttg
     7141 tggtggattc gtggttcggg gagcacggcg atccggccgt gatcctgccc cttgaagaat
     7201 ggctcgatgg agaaggaccg aatgtctcgt aaggaaaagg tgatcgtcgg catgtccggc
     7261 ggtgtggatt cgtccgtggc ggccttgctg ctcaagcagc agggctacga cgtggtcggc
     7321 ctgttcatga agaactggga ggacgacgat gacgacgagt actgttcgac acgtcaggat
     7381 ctggtggatg tggcctcggt cgccgacgtg atcggcatcg acctggaggt cgtgaatttt
     7441 tcaaaggaat acaaggaccg ggtgtttgcc gatttcctgc gggaatacga agcgggccgc
     7501 acacccaatc cggacatcct gtgcaactcc gagatcaagt tccgggcctt cctggaccac
     7561 gccctggccc tcggcgccga ccgcattgcc accggtcact atgcccaggt gcgggaatgg
     7621 accaacgacg ggcgtaccga attccagttg ctcaaggccg aggacggcac caaggatcag
     7681 agttacttcc tgtaccgcct caatcaggcg cagctttcca agacgctgtt tcccatcggc
     7741 catctctaca agcgcgaggt gcgccgcatg gccgaggccg ccggcctgca cgtctttgac
     7801 aaaaaggatt cgaccggcat ctgcttcatc ggtgagcggc cgttccggga gttcctgtcc
     7861 cgctatctgc cggccagacc gggcgagatc cgcacgctgg agggtgacaa ggtgatcgga
     7921 cagcatcagg gcctgatgta ccacaccctc ggccagcgca aggggctgca gattggcggc
     7981 atcaagggca accaagacga ggcgggcgaa cacgacgcct ggtacgtggc acgcaaggat
     8041 gtggcgaaga atgtgctgta tgtggtgcag ggccacgacc atccggccct gctccgggat
     8101 cggctggcgg cggcagagct gtcctgggtg tccggtcgcg atccgcgcac ccattgggtg
     8161 tacaccgcca agccgcgtta ccgcacgccg gacgcgccct gcgagatcga cgcattggcg
     8221 gacggccgcg ccgagatcgc ctttgcccag ccccaatggg cggtgacacc cggtcagagc
     8281 gtagtgatct acgagagccg ggtgtgcctg gggggcggaa tcatcgtgga atgagggccc
     8341 tgaacctcgc ttgagacgct gaaaacggtt cgcgcgggcg gtccgttttc attgagtctt
     8401 gcagcggggc cgggcgctcc ggcgtcaggg gttctgggcg acccggaaga gggcatagga
     8461 ccagttgttc tcccacgcac cgcggaccag attgggatat tccgcgcggc ccaggctgcc
     8521 gttgtagcgg cccagggcgc ggaacaggtc gcctcgttcg atatccaggt agtgccgcag
     8581 gatcgtgcag ccgaagcgca ggttggtgcg catgtggaag aggttgctgt ccgaggtgcc
     8641 gatcaggtcg acccagaagg gcatgacctg catgtagccg cgggcgccgg ccgtggacag
     8701 cgcgtacttc ttgaaaccgc tttccacctg gatcagcccc agcacgagtt gcggatcgag
     8761 gccggcgcgg gtggcttcgt aatggaccgc ccgcaggaat tcgaggcggc cggcctcgtc
     8821 cggcatgcgg cgggcgaggc ggcgggacat ttcgtccagc cagcgggccc gctccggcga
     8881 gcgggtgaag cgggtgtcgg gcggcgcctg gtcgctcacc gcggcatgca gggctgcacg
     8941 cacgctggcg gcgaggggtt cgtagatctg gttaccggcc tgagcaccga agcacccacc
     9001 cgccagcgcc agggcggcgg cgcatcggag gaggcgcggg ctcaggtggc gcatgctcaa
     9061 gccttgaggc gggccagcag attggcggag gcttctgcca ccggccaacg ggtggcttcg
     9121 gcgtcgcggc ggccctggta ttccaccacg ccgtccttga ggccgcggtc gccgatgaca
     9181 acccggtgcg gggtgccgat cagctcgttg tcggccagca gggaacccgg tcgctcgtcc
     9241 cggtcgtcca ggaagacatc gatgccggcg gcgctcaggt cggcgtagag ggtgtcggcg
     9301 gcggcccgca cggcgtcgga cttggcgtag cccatgggca cgatggccac ctggaagggg
     9361 gcgatggcgt cgggccagat gatgccgcgg gcgtcgttgt tctgctcgat ggcggcgccg
     9421 aggatgcgcg atacgccgat gccgtagcag cccatctcca tgacctgttc cttgccctgc
     9481 tcgttgagga acttgcagcc gagggcttcg gagtacttgg tgcgcagctg gaagatgtgg
     9541 cccacttcga tgccgcggca gatctcgagg gtgcccaggc cgtccggcga cggatcgccg
     9601 gcgaccacgt tgcgcaggtc ggcgatctcg gcctcgggga agtcgcggcc gatgttcact
     9661 ccggtgaagt ggtgatcgtc gtcgttggcg ccggtgacga aatcggccat ggccgccacc
     9721 gtgcggtcca tcaccacgcg gccaccgaag cccaccgggc cgagggaacc gggcgcggcg
     9781 ccgaaggctt cgcggatgga ttcgggggtg gcgaaggtga ggggattgcg cacgccggcc
     9841 agcttctggg ccttgacctc gttgagttcg tgatcgccgc gcaggagcag cagtaccggc
     9901 cggccgccga cgctgtcgtc gccttgcacg actaccgcct tgacggtggt gtctaccggc
     9961 accttcagga aggccgccac gtcttcgatg ctcttgacgc ccggcgtgga caccttggtc
    10021 aagggcgccg ccggcaccgg gcgcggcgtg gccggagcaa gggcctcggc cagctccacg
    10081 ttggcggcgt agtcggagtc ggggcagtag gcgatgtcgt cctcgccggt ctcggcgatg
    10141 acgtggaact catgggaacc ggtgccgccg atggaaccgg tgtccgccgc caccgcgcgg
    10201 aacttcaggc ccagacgggc gaagatgcgg tggtaggtgt cgtacatgtt gcggtattcc
    10261 cgctgcagat cctcgaagga ggtgtggaag gagtagccgt ccttcatcac gaactcgcgg
    10321 ccgcgcatca cgccgaagcg ggggcggatc tcgtcgcgga acttcatctg gatctgatag
    10381 aagtgcttgg gcagctggcg gtagctgcgc agctcgcggc gggccacgtc ggtgatgact
    10441 tcttcgtggg tcgggccgat cacgaaagcg cggtcgtggc ggtccttgaa gcgcagcagc
    10501 tcggggccat agaaatccca gcggccggat tcctcccaca gttcggccgg ctgcacggcc
    10561 ggcatcagca cctcgatggc gccggcgcgg ttcatctctt ccctcacgat ggcctccacc
    10621 ttgcgcaggg cacgcaggcc caggggcatc caggtgtaga tgccgcccgc caggcgtttg
    10681 atcaggccgg cccgcagcat gagcttgtga ctgacgattt cggcgtcgga cggggcttcc
    10741 ttgagggtcg agaggaagaa acgggaggcg cgcatcgggg aacatccgtg aaagaagcaa
    10801 cctccaattc tatcgtggac ccaggtcccg gcacgctttt gttgcgatgc accgttatgg
    10861 ggcttcgtct ttcgcgggcc ggggaaatat ggaagaattc tcaccagttc atgaattgaa
    10921 ggttgcacca tgctcgaccg tgaaggctat cgccctaacg tcggcatcat cctggtgaat
    10981 gcgcgtaacg aggtgttctg gggcaaacgg atacgagagc attcctggca gtttccgcaa
    11041 ggcgggatca agcacggtga gtcgccggaa caggcgatgt accgggaact ctacgaggaa
    11101 gtgggcctga agcctgagca cgtccgtatc atcggccgca ctcgcggctg gctgcggtac
    11161 gacgtgccca agcactggat caagcgcgag tggcgcaata cctaccgcgg tcagaagcag
    11221 atctggtatc tgctgcggct ggtggggcgc gactcggacg tgagcctgcg tgcgagcaat
    11281 cacccggaat tcgatgcctg gcgctggagt tcgtattggg tgccgctgga ggcggtcatc
    11341 gagttcaagc gcggcgttta ccagtcggcc ctgcttgagc tggcccggct ccttttccgt
    11401 gaccggttct tcaacattcc cgacagttac ctgccgcacc cggcagagcc gctggaaggt
    11461 gaaggggccg atgcggggcc ccgtcgttga cggcgtgcgc gcggcgtcgg ccggttcgat
    11521 tttgcgccgc gggaaacttg tccgtgccgc gcaagtctga tcgaagcgtt tgtagggcgt
    11581 cgcgtcagct ttgagatgcg gcgcgggcgt tccggggcag gatgccctgg aatctttgca
    11641 gggcacgctt gggaggagac accatggagc gcatgcacta tcagccgatc agccagttca
    11701 aggtcagccg cgggacctgt gcggtcgccg aggcctgggg cctgacacgg gttaccgcgt
    11761 cgtcgccggc cgtggaggtg atgaccgacc tgcgacggat tcccgccgcc accatcggac
    11821 aggacgtttc cctcgcggag gccaatcacg ccatgatcct gcgcggcgtc cggctcctgt
    11881 tcgtgaccga cgcggcgcgt cgggtggtgg ggatcgtcac ggcaacggac ctgctcggcg
    11941 agcgtcccac ccaggtggcg cgggagcgct ccctgcgata tgaggagctc accctcaacg
    12001 agatcatggt gccctccgag gcgctcgatg cggtcagcat gagcgacgta ctgcgcgccg
    12061 aggttggaca cgtggtggcg acgttgaagt cgtccgggcg tcagcacgcg ctggtcgtgg
    12121 aagagacgcc cgagggacag ccgacgatcc gcggaatctt ctcggcatcg cagattgccc
    12181 ggcagatggg catcccgctg cagacgaccg aagtggcgcg cacctttgct gaaatcgaag
    12241 cggcgattgc ccattgaggg gcgtggccag aggatatgtt ttgaatcacg gtgtgcacgt
    12301 agcgggagcc aggctggcgg gacgttccgg acgtctggcc agcctttttt tcggactttg
    12361 tgcagggctg ctggcctcgg tcggggcggt cgcccaggtc gcccagcagt cgctcttcac
    12421 gtcgcccgag ccgcccgcgt gggcggaatc gccggtggag attcccaagg agctgccgga
    12481 tgacgcggcg cttgcgccgt tttcagtcag ccgggcggcg acggcgacgt ttctgatcga
    12541 tacctccagc gtgacgcgcg gcgaggacgg catctggcga tttgcgctgg tgatccgtac
    12601 tgctggcggc gccgagaaca tcacgtatga agggatccgt tgtgcgacgg cggagcgtcg
    12661 actgtatgcc atcggtcggc gtggcgaagg ctggaccgct tcgcgcaacg acgcatgggg
    12721 gcgcatcgcc gaaagctcgc tcaaccgtta tcacgcggcc ctgttcaagg actacttctg
    12781 tccgcccggg ctggcccaac ccagtcctgc cgaggcggtt tcccgcctgc gcagcggaat
    12841 gcccatcctt ccaaccggaa acctgacgcc atgatcgacc agctcattct cggatttgac
    12901 cgtgccttgc agaccgtgtt tgccagtgca tccaccgtcc gcccgacccc gggcgaggac
    12961 cttcccgaga cggaactgag cgatgcggag cggcgccacg ccgccgcgct gatgcggatc
    13021 aatcattgcg gtgagatctg cgcccaagcc ctctaccagg gccaggcgct gatgtctgac
    13081 aacgacggca tccggcgtgc gcttgagtct gccgcccagg aggaaaccga gcacctggcg
    13141 tggacggagc ggcgcatcgc cgaactgggt gggcgcaaga gtctgctcaa tccgctctgg
    13201 tacgggggct cgctggccat cggcatggct gcggcgaaga tcggcgacgg tttcaacctg
    13261 ggattcctgg ccgaaaccga acggcaggtg gaggcgcacc tgaaaagtca tctggacgat
    13321 ctgccccaga aagacggacg gacccgggcc atcatcgaac agatgaagac ggacgaggtg
    13381 gcgcacgcgg agacggccgt gcggttgggg gcagtgccgc tgcccccgcc cgtgcgcggg
    13441 gcgatgaagc tcatgtcgcg ggtcatgacc cgcacggcct actggctgta gggcggtgtc
    13501 cggcgagccg ctccggcggc gcggtgcggg cggggctgac cgcgtttatg cctcgacgat
    13561 gtcccaggta tgctcgatct cggcggtctt gccgagcatg atggtggccg agcaatactt
    13621 ttcatacgag agcttgatgg cgcgctccac gacgtccggc ttgaggttgc ggcccgtcac
    13681 ggtgtagtgg aaagcgatcc gtgtgaacac tttcgggtcg gtctcggccc ggtcggcctt
    13741 gagactgacc ttgcagtccg tcacgtcctg gcggctgcgc ttgaggatga gcacgacgtc
    13801 gaaggccgtg cagcctcccg cgccggccag catgagttcc atcggacgag gggcgatgtt
    13861 gcgccctccc gcctccggcg cgccgtccat catgaccgtg tggccggaac ctgtttcggc
    13921 caaaaaactc ataccgtcca cccacttaat gacgcattcc attgcttgct tcctcgcgtg
    13981 tcgtgtgaag gcgcggtgcg gcgagcccgt gccgtagagg cgccattgta cccggcctgc
    14041 ccagccttcg cgggcaggtg ttgtgcgctg cagcatactg gcttttgaaa cgggcttgct
    14101 tatgccgaaa tcgctgtttt tcattcaaaa aaaataatta aaaacatgga gttgcggtga
    14161 atttttgaaa taaatagaat ttatcgaacg attaaaatat ttatgacgca ttgcacaaaa
    14221 agtgcttgca tcacaggtcc ggtcgaccca taatgaactc agtccagtcg gtttgtttca
    14281 cctgacccga ctgaagcggt gtctcctcca ccctcctcct ttggtgtgga tttagcgcag
    14341 atctccccga tctgcgcttt tttttatctt gagcaagggt ttgacatcca ggcgttcgca
    14401 gggatagaat ctgcggcttt ccgaattgtg gtcggtcccg agcgggacaa gtctgaggaa
    14461 ttcttcaatg aaaacgtttt ctgccaagcc gcatgaggtc aagcgcgact ggttcgtggt
    14521 ggacgcgacg gacaaagtcc ttggtcggct ggcttccgag gttgcgcgtc gcttgcgcgg
    14581 caaacataag cccgaataca ccccccacgt ggataccggc gactacatcg tcgtcgtcaa
    14641 cgtggacaag ctgcgtgtga ccgggaataa ggcgctggac aagaagtact accgtcactc
    14701 cggctacccc ggcggtatct acgaaaccaa cttcaccaag ctgcagcagc gcttcccggc
    14761 gcgcgtgctt gaaaaggccg tcaagggcat gctgcccaag ggcccgctcg gctacgccat
    14821 gctgaagaag atgaagtgct acgccggtgg cgagcatccc cacgctgcac agcagcccaa
    14881 agttctcgag atctaacagg agccgatgat ggctgtgact tacaactacg gtaccggtcg
    14941 ccgcaagtcg gcggtggccc gtgtctttat caagaagggt accggcaaca tcgtcgtgaa
    15001 cggcaagccg gtggatcagt ttttctcccg cgaaactggc cgcatgatcg tacgccagcc
    15061 gctggtgctg acgaactttg ccgacagctt tgacatcctg gtgaatgtca ccggtggtgg
    15121 tgagtccggc caggctggcg cggtccgtca cggcatcacc cgcgccctga tcgactacga
    15181 tgcggaactc aagtccccgc tgaagaaggc cggtctggtg acccgcgatg cccgtgaagt
    15241 cgagcgtaag aaggttggct tccgcaaggc gcgccgtcgc aagcagttct ccaagcgtta
    15301 agcgctggat ggttttgtcg aaaggccgcc ttcgggcggc tttttgtcat cttccggccg
    15361 taatctggtg gtccgtgtcc gggttgtggt caaatgcggc actgatttcg tgttccagtt
    15421 cggagagtta gcatgatcaa ggtgggtgtg gtcgggggga ccgggtatac cggcgtggaa
    15481 ctgctgcggc tgctggccca gcatccggag gcagagatcc gtgcgattac gtcccgcggt
    15541 gaggcgggca tgccggtggc ggatctgttt cccagtctgc gcaagcgtgt cgacctgtgc
    15601 tttgtggatc cccaggcggc tgatctggca tcttgcgatg tggtgttctt cgccacgccc
    15661 aacggcattg ccatgcaaca ggccgccgcg ctggtcggtg cgggcgtgcg tgtgatcgat
    15721 ctggcggcgg atttccggat taaggatgtg gccgaatggg agaagtggta cgggatgaag
    15781 cacgcctgtc cggaactggt cgctgaagcg gtgtacggcc tgcctgaagt gaatcgcgaa
    15841 gcggtacgcc aggcacgggt ggtcgccaat cccggctgct accccactgc ggtacaactg
    15901 ggttttctac cgcttgtgga ggccggggtg atcgagctgg atcatctggt cgccgacgcc
    15961 aagtccggtg tgtcaggcgc gggtcggaag gccgaaacgc atatcctgtt ttccgaggcg
    16021 gcagacaatt ttcaggcgta cggcgtagcg ggacatcgcc atctgcctga gatccggcag
    16081 ggattggccg ggtttgcagg gcaggccgtg ggactgacct tcgtgccgca cctgaccccg
    16141 atgattcggg gtattcatgc cactctctac ggcaaactca aaaaagacgt ggatctgcag
    16201 gcgttgttcg aggagcgctt tgcgggagag cccttcgtcg atgtgctgcc caagggttcc
    16261 catccctcga cccgttcggt ccgttctgca aacatgtgtc gaatcgcggt gcatcgacct
    16321 caggggggcg ataccgtcgt ggtgctgtcg gtcatcgaca acctcgtcaa gggggctgcg
    16381 gggcaggccg ttcagaatat gaacattctc tttggcctgc cggaaacgac cgggttgggg
    16441 caggtgccgg tgatgccgta gaacttatcg cccgtttccc ggtcaactag ccactcgtgt
    16501 ggcttgaccg gcttctggcg gctacccata atttgaagac tgacctgaag gagttccccc
    16561 atgaatgctg taaccgagat gccggcaccg ctggtgttca ctgatagtgc cgtcggcaag
    16621 gtcaaggatc tgatcgccga ggaaggtaat cccgagctga agcttcgcgt tttcgtgacg
    16681 ggcggcggtt gctccgggtt ccagtacggg ttcacgttcg acgagatccg taacgacgat
    16741 gacaccgtga tggaaaagga cggcgtgatg ctgttgatcg acccgatgag ttaccaatac
    16801 ctcgtgggtg cggagatcga ctacacggaa ggtctggaag gctcccagtt cgtgatccgc
    16861 aatccgaatg cgaccagcac ctgtggctgt gggtcgtcct tctcggcctg acctctccgt
    16921 acgcattgca tgaaaagggc gcctgttggc gccctttttt gtgcctgtga ccgttggatc
    16981 agtcgaagcg ggccgtggtg atctggcggg ctgcagtcag ttgggcgttg agttgagcaa
    17041 cgtcatggag gaaactgccc agcttctggc gatcaagagg ctgggcggca ggcaggctga
    17101 ccttaagcgg gtcctgatgg atttcgttga ttcggaactc atagtgcagg tgtggtcccg
    17161 tggcccagcc ggtcgagccc acgtagccga tcacggtgtt ctggctgacg cggtccccct
    17221 tgcgcaatcc acgcgcgaca gcgctcaggt ggccgtaggc cgtggagtag cggtcctggt
    17281 ggcggagcac cacgaaattt ccgtaaccgt tttgccagcc ggcaaagtcc acgatgccat
    17341 cggcggttgc atgcacggcc gtgcccgttg gagcgccgta gtcgacgccc ttatgctcgc
    17401 gccaagtacc cagaatcgga tgcaggcgca tcgaaaatcc ggaagtgacc cgtgagaatt
    17461 ccagtggcga gcgcaaaaag cccgcgcgca ggctgcgtcc gtccggtccg tagtattcgg
    17521 tcttgccgtc cgcgtggcgg aacaggtagg ccgagtgttt cacgccctgg ttgaagaatt
    17581 cggcggcgag gatgcggcca gtgcggatcg cctgtccgtg gtggtaaaag acctcgtaga
    17641 tgacgctgaa acggtcaccc ttgcgcaggt cggagtggaa gtcgatctcg ctgccgaaca
    17701 tccgggcgag ttcttcggcg atgctgtcgg gcaggccggc cgcgtcggtg gcggcaaaga
    17761 gggagctctg gatggtgccc gactgcatgt ggatgcgtcg gtccagctcg agggcctgct
    17821 tgcggatgac gagctggccg ttttccgcgg tcagcacgac ctgatcatca ttgcccgggc
    17881 ggaggaaaga ggcggattgc aggcggccgt ccgtggttgt ggcgaccgtg accaccatgc
    17941 ctggccggaa agctgttccc agatcgcgca tcagggcagg actgccggcg tgggcggtca
    18001 gggacgtgtc cgagacgccg agtcgcttga gcagagcccc gagcgtctcc ccggcgagga
    18061 ctctgtcttc acggatgaaa gggtgctcgt cacggcccga aatgtcgaag gccgggcgtt
    18121 ccagctgtgt gacgatcgtc tctgccggcg gcggagcgtc ctgaccggga gccacggcga
    18181 gcgcggctgc catgcccgtg aatactccgg cgccgagcgc catcgacttc agcagcgcct
    18241 tccgatcgcc gtcacgcaac aatttgagat gcgctagaat ccccgtcttt tctctaagca
    18301 ccggactttc caagcaaaat cgagccgcgg agtctaccaa aaagcgcgcg ggcctgcctt
    18361 ggtctggaac agatatgacg gaaatcacgc aagcactcga actcatcaag cgcggctcgg
    18421 acgagcttct ggtcgaatcc gagctggtgg acaagctcaa gtcgggccgc ccgctgcggg
    18481 tcaaggccgg cttcgatccg acggcgcccg atctgcatct gggacataca gtactcatca
    18541 acaagctgcg ccacttccag gatctggggc atcaggtgct gttcctgatt ggcgacttca
    18601 cggggatgat cggtgatccg tcgggcaaga atgcgacacg cccgcccctg tcccgggagc
    18661 aggtgcttgc caatgcccag acctatcagg agcaggtgtt caagattctc gatccggcgc
    18721 gcaccgaggt ggtcttcaac tccacgtgga tggaatccct cggcgcgtcc ggcatgatcc
    18781 gtctggcggc gcagcacacg gtggcccgca tgctggagcg ggacgatttc tccaagcgtt
    18841 ttggcaacca gcaacccatc gccattcatg agttcctgta ccccttgtgc cagggctatg
    18901 actcggtcgc attgaaggcg gatgtggaac tgggcgggac cgatcagcgc tttaatctgc
    18961 tgatggggcg ggagttgcag aagaatgccg gtcaatcgcc ccagtgcgtg ctgaccatgc
    19021 cgctcttgga ggggctggac ggcgtcaaca agatgtcaaa gtctctgggc aactacatcg
    19081 gaatctatga gcccccgggg gagatcttcg gcaagatcat gtccgtctcg gacgatctca
    19141 tgtggcgcta ttacgacctg ctatcgttcc gctcatcggc cgaggtggcc gggttgaagg
    19201 aggcggtaca gaccgggcgc aatccgaggg atgtcaaagt ggccctggcc caggagatcg
    19261 tcgcgcgctt ccatggtgag ccggcggctg aagcggcgct cacggacttc gaggcgcgct
    19321 ttcagcgcaa tgcgatcccg gacgatctgc cggaagtggc gatctccggg ggcgaaaccg
    19381 gcctcagcgt ggtcgccgtg ctcaagcagg cgggactcac tggcagcacc tcggaggcga
    19441 tgcgcatgat cgatcagggg ggcgttcggc tgaatggtga aaaggtgtcg gacaaaggct
    19501 tggtgctgaa ggcgggggct gtggtcgttc ttcaagtggg caagcgaaaa tttgcccgcg
    19561 tcacgctgcg ctgatttatt tttccaatgt tgttgacgtc gttgaatagt tctttataat
    19621 gc
//
LOCUS       DAOQPA010000063        19616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1381_length_19616_cov_18.468943, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000063 DAOQPA010000000
VERSION     DAOQPA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_19616_cov_18.468943"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKX01_10105"
     CDS             complement(<1..1175)
                     /locus_tag="PKX01_10105"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HMZ76522.1"
                     /translation="MHRLWLIFTQAVTVTVAALFVVGTLKPEWLGGRPGGASVIIQEA
                     VAPAAPSVAVAPAHAAASYADAARAALPAVVHIFTSKEVRGQRSPFANDPLFRHFFGD
                     RLDNQPQQRASGLGSGVIVSPEGYILTNNHVIEAADEIEVALQDGRKLPARIVGRDPE
                     SDLAVLRIKTDVRLPAITFGRTEALHVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLG
                     INTFEDFIQTDAAINPGNSGGALVDASGHLVGINAAIYSRSGGSLGIGFAIPVSLARN
                     VMEQIIATGTVTRGWIGVEVQEITAELADSFGLPGTQGTLISGVMRGGPADRAGIRPG
                     DVLLDVAGHAVNDPQSMLEAVAALPPGNRAPLQIRRGREKVDVQVEIGRRPALARSE"
     gene            1241..1984
                     /locus_tag="PKX01_10110"
     CDS             1241..1984
                     /locus_tag="PKX01_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMZ76523.1"
                     /translation="MDRAELQQYLETVLEVGRFKDYCPNGLQVEGRGEIRHIVCGVTA
                     SRALLDAAVARGADAVLVHHGYFWRGEDGRITGLRKARLATLLCHDINLFAYHLPLDA
                     HPELGNNAQLGARLGLTAAGRFGDQELGWLGCTAPTTLAGLAARVAEGLGRTPLVLGD
                     PARPVRRVGWCSGGAQGYFEAAIAAGVDCFISGEASEPTTHLARESGVAYLAAGHHAT
                     ERYGVQALGAHLAERFGLKAEFVEIDNPV"
     gene            complement(2027..4036)
                     /locus_tag="PKX01_10115"
     CDS             complement(2027..4036)
                     /locus_tag="PKX01_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76524.1"
                     /translation="MPRTSLLVIAVLAALPVAPVRGAALGELSARSALGAPLNAEIRI
                     TPAPGEQIDGQCIRIVRGQTDDLPWLAGARVRLEGERLIITTRDPVNHPAAMLGVHIG
                     CGIELRRDYALLLQPPLESPAVTAAVVTPDAPAPARAPSTPPAAARPRPVGQVIVLSE
                     DTPLARLADERAGPSPARRQRFIERLVMANPDVFPSGRADEGLVIAAGTRLKTTPPAL
                     PAHHADTPPRRTDTVARAQAAPAAPTSPAQATRPSTRREPAPRQDRLVVAGTAGADDM
                     PLRLSLQLANPPRDDAPGDAVRGPIRQEQRTLTEIDDRITAQLELNEKIKRLEEYQEL
                     LKRKIADLDAGPPGQAAPTAAPPPAPAPAAAPRVSTPAAVAPPESAAEGLPAWALPAG
                     AGLVTLLLGGGALVWWRRRNLVVPEDSLEPISVGAESTTGLHAETPPVFVTTMGPPSI
                     LIPSPAAAPAPQSAMPVREMPALDIDLAPKGDWSEPTFAPTHSVPFDEAVDEQDSALE
                     LAEIMMSFGRTQGAAETLADYIRNNPRQAVKPWIKLLEVYHLAGMRAEFEALTRQLNK
                     TFNVKMVSWHDYRAPETAPDSVEQLPHIVRRLEETWNTADAQAYIHQILRDNRDGTRQ
                     GFPLGVVDDLLMLLAVLDDHLGPYRQAAETPDNAPAPADTHKAAA"
     gene            4252..4797
                     /locus_tag="PKX01_10120"
     CDS             4252..4797
                     /locus_tag="PKX01_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HMZ76525.1"
                     /translation="MSLPPSSAARRRVHARRIEIEGFLREDGLLDLDARLTDVKDQDY
                     VIASGLRKAGEPIHDLFLRVTIDTQFNIVGACAVSARVPYPGGCETITPAYRQLVGLN
                     LVKGFRKTVGEMFADVRGCSHLSELLLSLPTAAIQTLATFRRDTEDTDGKPFQLDRCH
                     ALETTTQTVLRYYPRWYRSGD"
     gene            5078..6238
                     /gene="sucC"
                     /locus_tag="PKX01_10125"
     CDS             5078..6238
                     /gene="sucC"
                     /locus_tag="PKX01_10125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMZ76526.1"
                     /translation="MKIHEYQGKQILKKFGVTVPRGIHCTTVDDAVKAAETLGGKIWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVRQYAGQILGMQLITHQTGPEGQKVRNLLIEEGA
                     DIKHEYYVAALTDRATQKVAMMASSEGGMDIEEVAHKTPEKIIKVFVDPAVGLTDEQA
                     LTLAKGIGIPEGSIAQAVSTLKNLYTCYMDTDASLAEINPLIHEGDGTVKALDAKFNF
                     DSNALYRHPEIVEMRDLDEEDADEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATTEKVTEAFKIMLKNPKVKGILVNIFGGIMRCDTIATGVVT
                     AAKEVHLSVPLVVRMKGTNEEIGKQILRDSGLPIISADTMAEAATKIVAAVQ"
     gene            6250..7143
                     /gene="sucD"
                     /locus_tag="PKX01_10130"
     CDS             6250..7143
                     /gene="sucD"
                     /locus_tag="PKX01_10130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMZ76527.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTEKCIEYANGKNCFVAGVNP
                     KKAGEKILDVPIFGTVKEAAAETGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNKMKAKEAAGGKKTLLLGPNCPGLITPEEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMRMFNDDPDTDAVIMIGE
                     IGGPDEAEAAQWCKANMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAIME
                     ECGFTVTRNPSEMGKLLKALL"
     gene            7272..7976
                     /locus_tag="PKX01_10135"
     CDS             7272..7976
                     /locus_tag="PKX01_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ76528.1"
                     /translation="MLSTPEFLTAAFWAGFVQIVAIDLVLSGDNAVVIALACRRLSPA
                     QRRIGVLWGVVGAVALRVVLTAFAASLLGYPWLKLLGGLLLLWIGVKLLLPDDDGGHE
                     IDAATSVSGAVRTIIVADFVMSVDNVIGVAGAAGDSLVLLGLGLAFSIPVIVWSSQLI
                     MKSMERFPVIVSLGAALLGWVAGSMMLSDPGLRPWVDGLPGWARMLAGVLGALGVIGV
                     ARVLAMRAGRSSEQAL"
     gene            8001..8771
                     /locus_tag="PKX01_10140"
     CDS             8001..8771
                     /locus_tag="PKX01_10140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ76529.1"
                     /translation="MADAYLPLRMAMLSDTGRVRVRNEDQVYCDASRGIALLADGMGG
                     HAGGALASRLAVAAWLAEVEDRPPGTIDETCMRAAVAAANQVVFDTACQDVLLRSMGT
                     TLVAVCFRTDGQMIACHVGDSRLYRLRGESLECLTRDHSVLREQCDAGMIEAESDGAP
                     ALRGLLTRAVGVDVQVQPDLIVTQVCAEDVFLLCSDGLTDMLPDREIFHVLDTLGANP
                     RLAAEHLVDLANDQGGTDNVSVVVVSLRSGGLPVSVIA"
     gene            8796..9494
                     /locus_tag="PKX01_10145"
     CDS             8796..9494
                     /locus_tag="PKX01_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ76530.1"
                     /translation="MPRLILSMDGLVLKEMVLEKERTTIGRKPHNDIQIDNLAISGEH
                     AAIVTILNDAFLEDLNSTNGTYVNGQPVKKHVLQNNDVVELGKYRLKFFTDSTVAAPM
                     DMTDSVAFATDLTRTDASRGAAPAPATEPPPSAAPQPAPAAMGMIQILSGSHAGRTLE
                     LSKSLTTLGKPGLQVAVITRRPHGYFITHVEGASFPVVNGRALDPQAQRLNDHDIIEI
                     AGVKMEFFSQPTER"
     gene            9649..10419
                     /locus_tag="PKX01_10150"
     CDS             9649..10419
                     /locus_tag="PKX01_10150"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HMZ76531.1"
                     /translation="MKVQVLGCSGGIGGRSQRTTSLLVDQDVLIDAGTGVGDLEFDEL
                     LKIDHVFVTHAHLDHIAMIPLLVDTVGEARSTPIVVHGSPETLRILRSHIFNWLIWPD
                     FSSIPDRGNPFLRFHEIHVGETVALDGGREITALPALHTVPAVAYRLACAHGSLVFSG
                     DTTYSEPLIAAINEIPDLRYLIVETAFPDALQGLALASRHLCPSLLALFLGRLTVNPE
                     VWITHLKPSRAQTTLDEIVSYCGRFEPRALYNGLEFAL"
     gene            10477..12825
                     /locus_tag="PKX01_10155"
     CDS             10477..12825
                     /locus_tag="PKX01_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HMZ76532.1"
                     /translation="MTSGMVRGSDVASRLAFFKGLQAVTNRIHATENIDEIIFELSSE
                     ICTLFAADRLTIYLVDDNRTTISSRVKTGLQAVRTIRLPIAENSVAGYVALTGRMLNI
                     HDAYDDRELKAVSERMEFRREVDDRSGYRCHQMLVAPIMRQEGADTLGVIQLINTRSG
                     EPFSSICEEGVQGLGQTLAIAFAQRRHTLAQPKSKFESLVSGGRLSEQELREALRKAR
                     EVGKSMERVLLDDLGIPLADVGAALERFFGVPYEPYRQDRIKPLDLLKNLKRDYVQQA
                     QWLPLEDTPEGMVILTVDPEQAVASRVVQNVFPKANPVYRVTTDHEFVQTLDQFFGAA
                     EDPSVTELLSDMADDDGEGTSTAEDVSAAADNELVKLVNRIIIDAHRQGASDIHIEPR
                     PGKEKTQIRFRKDGSLVPYIEVPSSYRSPIVTRIKIMCDLDISERRKPQDGKIKFRRY
                     GPLDIELRVATVPTAGGMEDVVMRLLANSEPLPLDQLGLLEDNYRRLKDTVSKPYGIF
                     FVCGPTGSGKTTTLHSILGSINTPDVKIWTAEDPVEITQKGLRQVQVNRKAGLDFPTV
                     MRSFLRADPDVIMVGEMRDKETVSIGLEASLTGHLVFSTLHTNSAPESIVRLLDMGMD
                     PFNFADALLGVLAQRLAKRLCGKCKRPYTPDEDEIRHLLDEYCEDLHQTPAFIEDPHG
                     AKEQVLATWRQRYAKDRGPFTLYAPVGCEHCADGYKGRLGLHELMVGTDRIKALIQER
                     ARVANLLSVALEEGMRTLRQDGIEKVLGGLTDLKQVRKVCIR"
     gene            complement(12856..14280)
                     /locus_tag="PKX01_10160"
     CDS             complement(12856..14280)
                     /locus_tag="PKX01_10160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ76533.1"
                     /translation="MRRSPLTFPARLTRPSIGGNAAGFTRRRFIGAAGASLALLVSRP
                     GTAAQTGILAVRVWPAQDYTRITLESREPLTFTHQLVHGPERLVVDLEGIELNGVLQS
                     LPTKISGQDPYIQLIRAGRNRPGVVRLVIDLKAEIVPQVFTLKPVGEYGHRLVLDLYP
                     VEPPDPLLALLEKLSPEEAAAGVPMPARPEPSSVRPTRPAEPGVARRQEAEPPPVRPQ
                     PAPEPPAARRKPAEDEGVSRLVTIALDPGHGGEDPGAVGAAGSFEKHVTLSIGRRLKA
                     RIDAEPNMRAMLTRDADYFVPLQQRVSKARRVQADLFVSIHADAFISPTARGSSVFVL
                     SERGATSTAARWLAQRENAADLIGGVNLGVKDEHLARTLLDLSQTASINDSLKVGKAV
                     LGELGTINRLHKGEVEQASFAVLKAPDIPSILVETAFISNPEEEARLNDEAYQDRMAD
                     AILRGLKRYFAKNPPTAKSKLASL"
     gene            complement(14244..14648)
                     /gene="tsaE"
                     /locus_tag="PKX01_10165"
     CDS             complement(14244..14648)
                     /gene="tsaE"
                     /locus_tag="PKX01_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMZ76534.1"
                     /translation="MPERLIILLHGDLGAGKTTLTRGLLRGLDYTGRVKSPTYTLVES
                     YKDSRIVVYHFDFYRFTHAEEFLEAGLDEYFQGPGLRLVEWPERAGPYLPPADLAIRL
                     RIADDGGRTLDLQALTPEGRTCVDRLSPSPRA"
     gene            14736..15839
                     /gene="queG"
                     /locus_tag="PKX01_10170"
     CDS             14736..15839
                     /gene="queG"
                     /locus_tag="PKX01_10170"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMZ76535.1"
                     /translation="MDTGHDVLSRKAEDLAALATRIRAWGEELGFGGIGISDVALEGV
                     EEGLAAWLAAGFHGSMDYMEKHGTKRTRPAELVAGTRRVISVRMDYLSAAAAHACEVL
                     ADRSLAYVSRYALGRDYHKVLRQRLQKLADRISAAAPHGYRVFTDSAPVMEVALAEKA
                     GLGWRGKHSLLLSREAGSFFFLGEIYTDLELPVDAPRSGHCGSCRACIDACPTGAIVA
                     PYRVDARRCISYLTIELHGAIPEVYRPMIGNRVYGCDDCQWICPWNRFAGQASVSDFT
                     PRNGLDRIKLLELFNWTPQIFNERMAGSPIFRIGYERWMRNLAVGLGNAPSSAEVVRA
                     LQSRRDDQSWLVREHVAWALERHGALRCGMSWQ"
     gene            15836..16609
                     /locus_tag="PKX01_10175"
     CDS             15836..16609
                     /locus_tag="PKX01_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76536.1"
                     /translation="MMSRKPAPEEERPPQTLAEVTAMESWRFDPLCIAEDDPKVSAPS
                     DSSNKFRIKMLMRDGDRRRETDLLIEKRYAWRGYGKLGSLEAPNQLTMNAELFGRVYA
                     TLTVNIDSPAGLALDKTYGAEAARLRSQGRKLCEFGRFAVDPSARSKRLIASLFNLLY
                     IYSYRVKGCTDILIEINPRHRDFYEKLLEFHQLGEVKHCARVNAPAMLMHLSCATIEA
                     RLKEIGGRWRELPDEKSIYKFAFTPEEESELIGRLDDDH"
     gene            16696..17505
                     /gene="murI"
                     /locus_tag="PKX01_10180"
     CDS             16696..17505
                     /gene="murI"
                     /locus_tag="PKX01_10180"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMZ76537.1"
                     /translation="MSETGTLPIGVFDSGVGGLTVVRALMERLPLESIVYFGDTARVP
                     YGVKSVATIEHFTGQITDHLLHQGVKMLIIACNTMAAVASHVVKDKAGDMPVLDVIDA
                     GARAAVAESRSRAIGVIGTPTTINSNAYARRMHALDPNVRVYSQACPLFVPLVEEGWL
                     EHEVTRLTAQEYLRPVLAENVDTLVLGCTHYPLIKPLLQDVVGPQVRLVDSAITVAEQ
                     AARALADLGIANPGDRLPTYRYTVTDIPLRFQTIGERFLGRSLGVVDKVDW"
     gene            complement(17598..18149)
                     /locus_tag="PKX01_10185"
     CDS             complement(17598..18149)
                     /locus_tag="PKX01_10185"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMZ76538.1"
                     /translation="MGHRLSKIYTRTGDAGTTGLGNGNRVSKNSLRIHTLGEVDEVNA
                     AVGVLLCEDLPEDVRVLLTNVQHDLFDLGGEVCIPGMEMITAKQVQYLEDELDRHNDD
                     LEPLKDFILPGGTRAAALCHQARTVCRRAERMIVALAQEEAVNDAPRQYLNRLSDLLF
                     VLGRVLNKAGGRGDVLWQKRKNA"
     gene            18313..>19616
                     /gene="alaS"
                     /locus_tag="PKX01_10190"
     CDS             18313..>19616
                     /gene="alaS"
                     /locus_tag="PKX01_10190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMZ76539.1"
                     /translation="MKSAEIRQKFLEFFASKGHQIVASSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIHYAWELLTEVFKLPKDKLTVTVYAEDDEAYDIWTKEVGVPADKVIRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGAHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRD
                     EAGVMHPLPKPSVDTGMGLERISAVLQGVHANYEIDLFQTLLKAAARETSAADLDSPS
                     LKVLADHIRACSFLIADGIIPGNEGRGYVLRRIIRRAIRHGYKLGVRAAFFHRLVPDL
                     VAEMGDAYPELKSHQQKVTDVLKQEEERFFATIEHGMAILEADLAEMSKAGSTVFNGD
                     TAFKLHDTYGFPLDLTADVCREHGVTVDNAAFDAAMARQKEQA"
BASE COUNT         3302 a         6186 c         6728 g         3400 t
ORIGIN      
        1 tattccgacc gggccagggc ggggcggcgg ccgatttcga cctggacgtc caccttctcg
       61 cgcccgcggc ggatctggag gggggcgcgg ttgccggggg ggagggcggc gacggcttcg
      121 agcatggatt gggggtcgtt gacggcgtgg ccggctacgt cgaggaggac gtcgccgggg
      181 cggatgccgg cgcggtcggc ggggccgccg cgcatgacgc cggagatgag ggtgccctgg
      241 gtgccgggca ggccgaagga gtcggccagt tccgcggtga tttcctgcac ttcgacgccg
      301 atccagccgc gggtgacggt gccggtggcg atgatctgct ccatcacgtt gcgggcgagg
      361 gagacgggga ttgcgaagcc gatgccgagg gagccgccgc tgcgggagta gatggcggcg
      421 ttgatgccca ccaggtggcc gctggcatcc accagggcgc cgccggagtt gccggggttg
      481 atggcggcgt cggtctggat gaagtcttcg aaggtgttga tgcccaggtg ggagcgcccg
      541 agggcggaga cgatgcccat ggtgacggtc tggccgacgc cgaaggggtt gccgatggcg
      601 aggaccacgt cgcccacgtg caaggcttcg gtgcggccga aggtgatggc gggcaggcgc
      661 acgtcggtct tgatgcgcag cacggccagg tcggattccg ggtcgcggcc gacgatgcgc
      721 gcgggcagct tgcggccgtc ctgcagggcc acctcgatct cgtcggcggc ttcgatgacg
      781 tggttgttgg tgaggatgta gccttccggc gagacgatga cgccggagcc gaggccggag
      841 gcgcgctgct ggggctggtt gtccaggcgg tcgccaaaga agtggcggaa caggggatcg
      901 ttggcgaagg ggctgcgctg gccgcgcact tccttgctgg tgaagatgtg caccaccgcg
      961 ggcagggcgg cccgggcggc gtcggcgtag gaggcggcgg cgtgggccgg ggcgacggcg
     1021 accgaggggg cggcgggggc gaccgcttcc tggatgatca ccgaggcacc gccgggccgt
     1081 ccgcccagcc attcgggctt gagggtgccg acgacgaaga gcgcggccac ggtgacggtg
     1141 acagcctggg taaagatcag ccagagacga tgcatcgtag aatcgggggc cgcgcgccgc
     1201 agcgctgcgg aatcatgcca agggattgaa aggacacgga atggatcggg ccgaattgca
     1261 gcagtatctg gagactgtgc tggaggtggg ccgcttcaag gattattgcc caaacggcct
     1321 gcaggtggaa ggtcgcgggg agattcgcca tatcgtctgc ggggtgacgg ccagtcgtgc
     1381 attgctggat gcggcggtgg cgcggggcgc cgatgcggtg ctggtgcatc acgggtactt
     1441 ctggcgcggt gaggacggtc gcatcaccgg cctgcgcaag gcgcgtctgg ccacgctgct
     1501 gtgccacgac atcaatttgt tcgcctatca cctgccgctg gacgcccatc cggaactggg
     1561 caacaacgcg cagttgggcg cgcgcctggg cctgacggca gcggggcgtt tcggcgatca
     1621 ggaactcggt tggctgggct gcaccgcacc cacgacgctg gcgggtctgg ccgcgcgggt
     1681 ggcggaaggg ctgggacgca cgccgctggt gttgggcgat ccggcgcggc cggtgcggcg
     1741 cgtggggtgg tgcagcggcg gcgcccaggg ttatttcgag gcggccatcg cggcgggggt
     1801 ggattgcttc atctccggcg aggcctcgga gccgaccacg catctggcgc gggaatccgg
     1861 cgtggcctat ctggcggccg gccatcacgc cacggagcgc tacggcgtgc aggcgctggg
     1921 cgcccatctg gcggaacgct tcgggctgaa ggcagagttc gtggagatcg acaacccggt
     1981 gtgaggcggg cggcccgatg ggcggcgcgg tggcgcccgc caggtttcag gcggccgcct
     2041 tgtgggtatc ggcgggggcg ggtgcgttgt cgggcgtttc cgccgcttgg cggtagggac
     2101 cgagatggtc gtccaggacc gccagcagca tgaggaggtc gtccaccacg cccagcggga
     2161 agccctgccg cgtgccgtcc cggttgtcac gcaggatctg gtggatgtag gcctgggcgt
     2221 cggcggtgtt ccaggtttct tccaggcggc gcacgatgtg gggcagctgc tcgacggaat
     2281 ccggcgctgt ttcgggcgcg cggtagtcgt gccagctcac catcttgacg ttgaaggtct
     2341 tgttgagctg ccgcgtgagg gcttcgaact cggcccgcat gccggccagg tgatagacct
     2401 ccagcagctt gatccacggc ttgacggcct gccgcggatt attgcggatg tagtcggcaa
     2461 gggtttcggc ggcgccctgg gtgcggccga aggacatcat gatctcggcc agctccagcg
     2521 cggaatcctg ttcgtccacg gcctcgtcga agggcacgga atgggtgggg gcgaaggtgg
     2581 gctcggacca gtcgcccttg ggggccaggt cgatgtcgag ggcaggcatt tcccgcaccg
     2641 gcatggcgga ctggggggcc ggcgcggcgg ccgggctcgg tatcaggatc gacggcggcc
     2701 ccatcgtggt gacgaagacc ggcggggttt cggcgtgcag cccggtggtg gattcggcgc
     2761 cgacggagat cggttcgagg gaatcctcgg gcacgaccag attgcggcgc cgccaccaca
     2821 cgagggcgcc gccgcccagc aggagggtga caaggccggc ccccgcgggc agcgcccagg
     2881 cgggaagtcc ttcggcggca gattccggtg gcgcgacggc cgccggtgtg ctgacccgcg
     2941 gggctgcagc cggggcaggc gcagggggcg gggcggcagt gggcgcggcc tgacccggcg
     3001 ggccggcgtc gaggtcggcg atcttgcgtt tgaggagctc ctggtattcc tcaaggcgct
     3061 tgatcttctc gttgagttcg agctgggccg tgatgcggtc gtcgatctcg gtgagggtgc
     3121 gctgctcctg gcggatcgga ccgcgaacgg cgtcgccggg ggcgtcgtcc cgtggcggat
     3181 tggccagctg caggctcagg cgcaggggca tgtcgtccgc gccggccgtg ccggcgacga
     3241 ccaggcggtc ctggcgcggt gcgggttcgc ggcgagtgct cgggcgtgtc gcctgagcag
     3301 ggcttgtcgg agctgccgga gcggcctggg cacgtgcgac cgtgtcggtg cgccgcggag
     3361 gcgtgtctgc gtggtgcgcg ggcagggcgg gaggcgtcgt cttgaggcgc gtgccggccg
     3421 cgatgacgag gccctcatcg gcgcggccgc tcgggaagac atccggattg gccatgacga
     3481 gtcgctcgat gaagcgctgg cgccgtgcgg gtgaggggcc ggcgcgttca tcggccaggc
     3541 gcgcgagcgg ggtgtcttcg gacagcacga tgacctgccc gacaggtctc ggacgggcgg
     3601 cggccggagg cgtcgacggc gcgcgcgccg gggccggtgc atcgggcgtg acgaccgcgg
     3661 cggtcacggc ggggctttcg agggggggct gcagcagcag cgcgtagtcg cgacgcaatt
     3721 cgataccgca gccgatgtgt acgcccagca tggcggccgg atgattgacg gggtcgcgcg
     3781 tggtgatgat caggcgctcg ccttcaaggc gcacgcgggc gccggccagc cacggcagat
     3841 cgtcggtctg cccgcggacg atgcggatgc actgaccgtc gatctgttcg cccggcgccg
     3901 gggtgatgcg gatctcggca ttcaggggcg cgcccagggc cgagcgggcc gacagctcgc
     3961 cgagcgcggc gccgcgcacg ggcgccacgg gcagggctgc gagaacggca atgacaagca
     4021 gggaagttcg gggcacgcgg ttcatcagga aatctggctg gagagcctcc ttgggcctct
     4081 tcatgattgt gcggcgcaaa attaaggtcg cgcaggggca ggtgctcaag tattctaaag
     4141 aactttccgg gccgtcgcgg gcgttgtgag caacgttcac ttttcgactg agtccttttg
     4201 cgtcactttt tgtgttttct gaaggccgac ctgcttcatt gttccgctgt catgtccctg
     4261 cccccgtcct ccgcggcccg gcgccgcgtc catgcccgcc gtatcgagat cgaaggcttc
     4321 ctccgcgagg acggtctgct cgatctcgat gcccgcctca ccgacgtgaa ggatcaggac
     4381 tacgtgatcg cgtcaggcct gcgcaaggcc ggtgagccga tccacgacct gttcctgcgg
     4441 gtcaccatcg acacccagtt caatatcgtg ggcgcctgcg cggtgtcggc gcgggtgccc
     4501 tatccgggcg gctgcgagac catcacgccg gcctaccggc agctggtggg gctgaatctg
     4561 gtcaaaggat tccgcaagac ggtgggggag atgttcgccg acgtgcgcgg ctgctcccat
     4621 ctgagcgagc tgctgctgtc cctgccgacg gcggcgatcc agaccctggc caccttccgc
     4681 cgcgacaccg aggacacgga cggcaagccc ttccagctcg atcgctgcca cgccctcgaa
     4741 accaccaccc aaaccgtgct ccgctactac ccgcgctggt atcgcagcgg cgactgagtt
     4801 tgcctgtccg gcgacggaac ctgctccggt ttcgttatat aattatggag tccacaatcc
     4861 ggcaaagggc cacatccagg cacggcgccg gcaggaaagg atggtttttc acgcaccgcg
     4921 gccctgtcgg tccggtgagc gaagagctcg agcggtgcgt acgtcggacg agggagaacg
     4981 gcgtacgggt cgcggggtgc agcgcaggcc ccgaatgagt cccatcaacc aagcgtgagc
     5041 ggcctgcaaa ggccaagact tcgatcgaag ggcaaccatg aaaattcacg aatatcaggg
     5101 caagcaaatc ctgaagaagt tcggcgttac ggttccgcgc ggtatccact gcacgaccgt
     5161 ggacgacgcg gtcaaggcag ctgaaaccct gggcggcaag atctgggtcg tcaaggcgca
     5221 gatccacgcg ggcggtcgcg gcaagggcgg tggcgtgaaa gtcgccaagt ccctggacga
     5281 agtgcgccag tatgccggcc agatcctcgg catgcagctc atcacccacc agaccggccc
     5341 ggaaggccag aaggtgcgta acctgctgat cgaggaaggc gccgacatca agcacgaata
     5401 ctacgttgct gcgctgaccg accgtgccac ccagaaggtc gccatgatgg cgtccagcga
     5461 aggtggcatg gacatcgagg aagtcgccca caagaccccc gagaagatca tcaaggtgtt
     5521 cgtcgatccg gccgtgggcc tcaccgacga gcaggcgctg accctcgcca agggcatcgg
     5581 gatccccgaa ggctccatcg cccaggccgt gtccacgctc aagaacctgt acacctgcta
     5641 catggacacc gacgcgtcgc tggcggaaat caacccgctg atccacgaag gcgacggcac
     5701 cgtcaaggcg ctggatgcca agttcaactt cgactccaac gccctctacc gtcaccccga
     5761 gatcgtcgag atgcgcgatc tggacgaaga agacgcggac gaaatcgaag cctccaagtt
     5821 cgatctggcc tacatctccc tggacggcaa catcggctgt ctggtgaacg gcgccggtct
     5881 ggccatggcc accatggaca ccatcaagct gttcggtgcc gagccggcca acttcctcga
     5941 cgtgggcggc ggtgccacca ccgagaaggt gaccgaagcc ttcaagatca tgctcaagaa
     6001 ccccaaggtc aagggcattc tcgtcaacat cttcggcggc atcatgcgct gtgacaccat
     6061 cgccaccggc gtggtcaccg cggccaagga agtgcatctg tcggttccgc tggtcgtccg
     6121 catgaagggc accaacgaag agatcggcaa gcagatcctg cgcgattcgg gcctcccgat
     6181 catctccgcc gacaccatgg ccgaagctgc gaccaagatc gtcgctgcgg ttcagtaagg
     6241 agtacgtgaa tgtccatcct gatcaacaaa gacaccaaag tcatcaccca gggcatcacc
     6301 ggcaagaccg gtcagttcca caccgagaag tgcatcgaat acgcgaacgg caagaactgc
     6361 ttcgtggccg gtgtgaaccc caagaaggcc ggcgagaaga tcctcgacgt gcccatcttc
     6421 gggaccgtga aggaagcggc cgccgaaacc ggcgccaccg tgtccgtgat ctacgtgccg
     6481 cccgcgggcg ccgcggctgc catctgggaa gccgtcgagg ctgacctgga tctggccatc
     6541 tgcatcaccg aaggcattcc cgtccgtgac atgctggaag tgcgcaacaa gatgaaggcc
     6601 aaggaagcgg ccggtggcaa gaagaccctg ctgctggggc cgaactgccc gggcctcatc
     6661 acgcccgaag agatcaagat cggcatcatg ccgggtcaca tccaccgcaa gggccgcatc
     6721 ggcgtggtgt cccgctccgg caccctgacc tacgaagccg tcgcccagct gaccgaaatc
     6781 ggtctgggcc agtcttccgc ggtcggcatc ggtggcgacc cgatcaacgg tctgaagcac
     6841 atcgacgtca tgcggatgtt caatgacgat ccggataccg acgccgtcat catgatcggc
     6901 gaaatcggtg gcccggacga agctgaagcc gcccagtggt gcaaggccaa catgaagaag
     6961 cccatcgtcg gcttcatcgc cggtgtcacc gcgccggccg gcaagcgcat gggccacgcc
     7021 ggtgcgctga tctccggcgg cgccgacacc gccgatgcca agctcgccat catggaagag
     7081 tgtggcttca cggtgacgcg caacccgtcc gaaatgggca agctgctcaa ggccctgctg
     7141 taatccggtt cgttcaggaa ccgatcgccg cgcccggggt tctgcccggg cgcggttttc
     7201 atttgtaggc gcgatccttg cgaggcgcga taaaatgcgt cgtgaatgac gcttccggat
     7261 tcctgcaatc catgctttca acccccgaat tcctgaccgc cgccttctgg gcggggtttg
     7321 tccagatcgt cgcgatcgat ctggtgctgt ccggcgacaa cgcggtcgtg atcgcgctcg
     7381 cctgccgacg tctgtcgcct gcccagcgcc gcatcggtgt cctgtggggc gtggtcggtg
     7441 cggtggccct gcgggtcgtc ctgaccgcgt ttgcggcgag tctgctgggc tatccgtggc
     7501 tcaagctgct cggcgggctc ctgctgctgt ggatcggcgt gaagctgctg ctcccggacg
     7561 atgacggcgg ccacgagatc gacgcggcca ccagcgtgag cggtgccgtg cggaccatca
     7621 tcgtggccga cttcgtcatg agtgtggaca atgtgatcgg tgtggcgggg gccgcaggtg
     7681 acagcctcgt cctgctgggg ctcgggctgg ccttcagcat cccggtgatc gtctggtcga
     7741 gccagctcat catgaagagc atggaacgtt ttcctgtcat cgtgagcctc ggcgctgcgc
     7801 tgctcggttg ggtggcgggc agcatgatgc tgtcggatcc gggcttgcga ccctgggtgg
     7861 acggcctgcc cggctgggcc cggatgctgg cgggcgtgct cggggcgctt ggcgtgatcg
     7921 gcgtggcgcg cgtgctggcg atgcgcgccg gccgttcttc ggagcaggcg ctctaggcgc
     7981 ccggcgcgga gcagatcgtc gtggccgacg cctatcttcc cctcaggatg gccatgctct
     8041 ccgacacggg gcgcgtgcgg gtgcgcaacg aggatcaggt ctattgcgac gcatcgcgcg
     8101 gcatcgcgct gctggccgac gggatgggcg ggcatgcggg cggtgcgctg gcgtcccggc
     8161 tggcggtggc ggcctggctg gcggaagtgg aagatcgtcc gcccggcacg atcgacgaaa
     8221 cctgcatgcg tgcggctgtt gcggcggcca atcaggtcgt cttcgacacc gcctgccagg
     8281 atgttctgct gcgcagcatg gggaccacgc tggtcgccgt gtgtttccgg acggacggtc
     8341 agatgatcgc ctgtcatgtc ggagattcgc gcctgtatcg cctgcgaggt gaaagcctcg
     8401 aatgcctgac acgggaccac agcgtcttgc gggagcagtg cgacgcgggc atgattgagg
     8461 cggaatccga cggggcgccc gcgttgcgag gcttattgac gcgcgcggtc ggcgttgatg
     8521 tgcaggtcca gcccgacctg atcgtcacgc aggtgtgcgc ggaggatgtc ttcctgctgt
     8581 gttcggatgg cctgacggac atgctgcccg acagagaaat cttccatgtg ctggatacgc
     8641 tgggtgccaa cccacgcctt gcggcggagc atctcgtcga tctggccaac gatcaggggg
     8701 ggaccgataa tgtatccgtg gtggtcgtat ccctgcggtc cggtggtctg cccgtctcgg
     8761 taatagcgta aggcttgttt ctgataggaa atgcgatgcc caggttgatt ttgagcatgg
     8821 acggtctggt tctcaaggag atggtgctcg aaaaggagcg caccaccatt ggccggaagc
     8881 cccataacga tatccagatc gacaatctgg ccatcagcgg cgagcatgca gccattgtca
     8941 ccatcctgaa tgacgccttt ctcgaggatc tcaacagcac caacggcacc tacgtgaacg
     9001 gccagccggt caagaagcac gtgttgcaga acaatgatgt cgtcgagctg ggcaagtacc
     9061 gcctcaagtt cttcaccgac agcacggtgg cggcgccgat ggacatgacg gattccgttg
     9121 ccttcgccac ggacctgacg cgcaccgatg cgtcacgcgg agcggccccg gccccggcga
     9181 cggaacctcc gccgagcgcg gcgccacaac cggcgccggc cgccatgggc atgatccaga
     9241 tcctgagcgg ttcccacgcc ggccggaccc tcgaactctc caagtccctg accaccctgg
     9301 gcaagccggg acttcaggtg gccgtgatca cccggcgccc ccacggttac ttcatcaccc
     9361 atgtggaagg ggcgagcttt ccggtggtca atggccgtgc cctcgatccg caggcccagc
     9421 gcctcaacga tcacgacatc atcgagatcg cgggcgtcaa gatggaattc ttctcccagc
     9481 ccaccgagcg ctgagtcccc tccctccggc gacccgtcga ttccttcgtg aaatttctaa
     9541 cgcgccagtc tggcggcaat ccgtatcctg acatattggc ggcgggggtt cctggcgagc
     9601 cggtgtgcat accctgtcgc catttctgcg agggatcggg ctgacgacat gaaagtgcag
     9661 gttctggggt gtagtggtgg catcggcggg cgcagtcagc gcacgacgtc cctgctcgtc
     9721 gatcaggatg tgctgatcga tgccggaacc ggtgtcggtg atctggagtt cgacgagctc
     9781 ctcaagatcg atcatgtctt cgtcacgcac gcccatctgg atcacatcgc gatgatcccg
     9841 ctcctcgtgg atacggtggg cgaggcccgg tcgactccca tcgtcgtgca tggctcaccc
     9901 gagaccctgc gcatcctgcg ctcccatatc ttcaactggt tgatctggcc ggatttttcg
     9961 tcgattcctg atcgggggaa cccctttctg cgctttcacg agatccacgt gggcgaaacc
    10021 gtcgcgctcg acggtgggcg cgagatcact gcgctgcccg cgctgcatac cgttccggcg
    10081 gtggcctacc ggcttgcctg tgcacatggc agtctggtgt tctcgggtga cacgacctat
    10141 tccgagccgc tcattgcggc gatcaacgag atccccgatc tgcgctacct gatcgtggag
    10201 accgccttcc ccgacgcgct gcaggggctg gcgctggcgt cgcggcacct gtgcccgagt
    10261 ctgctggcgc tgttcctcgg gcgcctcacg gtcaatcccg aggtgtggat cacccacctt
    10321 aagcccagcc gtgctcagac caccctggac gagatcgtca gctattgcgg tcgcttcgag
    10381 ccgcgggcgc tgtacaacgg actggaattc gcgctgtaag cgcccttaca ttcggcgccc
    10441 acccgggcgg gcgctgttct tgcagattac gacgggatga cgagcggcat ggtgcgtggc
    10501 agtgacgtag cgagcaggtt ggcttttttc aagggcttgc aggcggtgac caaccgaatt
    10561 catgccaccg agaacatcga cgagatcatc ttcgagctgt cctcggagat ctgcaccctg
    10621 ttcgccgccg accggctgac catctatctg gtggacgaca atcgcaccac catctcctct
    10681 cgggtcaaga ccgggctgca ggcggtgcgc acgatccgtc tgcccatcgc cgagaacagc
    10741 gtggcaggtt atgtggccct caccgggcgc atgctcaaca tccacgacgc ctacgacgac
    10801 cgggaactga aggcggtgtc ggagcgcatg gagtttcgcc gcgaggtgga cgaccgctcc
    10861 ggttatcgct gccaccagat gctcgtggcg cccatcatgc gccaggaggg cgccgacacg
    10921 ctgggggtga tccagctcat caacacgcgc agcggcgaac ccttttcgtc aatctgcgag
    10981 gaaggtgtgc aggggctcgg acagaccctt gccatcgcct tcgcccagcg caggcatacc
    11041 ctggcccagc ccaagtccaa gttcgagagt ctggtgtccg gcgggcggct gtccgagcag
    11101 gaactgcggg aagccctgcg caaggcgcgg gaggtgggca aatccatgga gcgggtgctg
    11161 ctggacgatc tcggtattcc gctggccgac gtgggcgccg ccctggagcg ctttttcggc
    11221 gtgccttacg agccctaccg ccaggatcgc atcaagccgc tggatctcct caagaacctg
    11281 aagcgggact acgtccagca ggcccagtgg ctgcccctcg aggacactcc ggagggtatg
    11341 gtgatcctca ccgtcgatcc ggagcaggcc gtcgcctcac gggtggtgca gaacgtcttc
    11401 cccaaggcga atccggtcta ccgggtcacg accgatcacg agttcgtgca gacgctggac
    11461 cagttcttcg gcgcggcgga ggatccgtcg gtgacggagc tgctctccga catggccgac
    11521 gacgacggtg aaggcacctc caccgccgag gacgtctccg cggccgccga caacgagctc
    11581 gtcaagctgg tgaaccgcat catcatcgac gcccaccgtc agggcgcctc cgacatccac
    11641 atcgaaccgc gacccggcaa ggaaaagacc cagatccgct tccgcaagga cggctcgctg
    11701 gtgccctata tcgaggtgcc gtccagctac cgcagcccca tcgtcacgcg catcaagatc
    11761 atgtgcgacc tggacatctc cgagcggcgc aagccccagg acggcaagat caagttccgc
    11821 cgctacgggc cgctggacat cgagctgcgg gtcgccaccg tgcccaccgc gggcggcatg
    11881 gaggacgtgg tcatgcgtct gctcgccaac agcgagccgc tgcccctgga tcagctcggg
    11941 ctgctggagg acaactaccg gcgcctcaag gacaccgtct ccaagcccta cggcatcttc
    12001 ttcgtgtgcg gccccaccgg ttccggcaag accaccacgc tgcattccat cctgggttcc
    12061 atcaacaccc cggacgtgaa gatctggacc gccgaggatc cggtggaaat cacccagaag
    12121 ggtttgcgcc aggtacaggt caatcgcaag gcggggctgg attttcccac cgtcatgcgt
    12181 tccttcctgc gcgctgaccc ggacgtgatc atggtgggcg aaatgcgtga caaagagacc
    12241 gtctccatcg gtctggaagc gtcgctcacc ggtcacctgg tgttcagcac cctgcacacc
    12301 aacagcgcac cggaatcgat tgtgcgtctg ctggacatgg gtatggaccc cttcaacttc
    12361 gccgacgccc tgctcggcgt gctggcccag cgcctggcca aacgtctgtg cggaaagtgc
    12421 aagcggccct atacgccgga tgaggacgag atccgccatc tgctcgacga gtattgcgag
    12481 gatctgcacc agacgccggc tttcatcgaa gacccccacg gcgcgaagga gcaggtcctg
    12541 gcgacctggc gtcagcgcta cgccaaggac cggggcccgt tcacgctgta tgcgccggtg
    12601 ggctgcgagc actgcgccga cgggtacaag ggccgtctcg gcctgcatga gctgatggtg
    12661 ggcaccgacc gcatcaaggc acttatccag gagcgggcgc gggtggccaa tctgctctca
    12721 gttgcgctgg aggagggcat gcgcaccctg cgccaggacg gcatcgagaa ggtgctgggc
    12781 ggcctcaccg acctgaagca ggtgcgcaag gtgtgcatcc gctgagatcc gcgatgccgc
    12841 ccgccgacgg gcgacttaca ggctcgccag cttggatttg gcggtgggcg gattcttcgc
    12901 gaaatagcgc ttgagaccgc gcaggatcgc gtcggccatg cggtcctgat aggcctcgtc
    12961 gttgaggcgg gcttcctcct cgggattcga gatgaaggcc gtttccacca ggatggacgg
    13021 aatgtccggt gctttcagca ccgcgaaact ggcctgttcc acctcgccct tgtgcaggcg
    13081 attgatggtg cccagttcgc cgagcacggc cttgcccacc ttgaggctgt cgttgatgga
    13141 ggcggtctgg gacaggtcca gcagggtgcg ggcgaggtgc tcgtccttga cacccagatt
    13201 cacgccgccg atcaggtctg cggcgttttc ccgctgggcc agccagcgcg ccgcagtgct
    13261 ggtggcgccc cgttccgaca gcacgaagac ggagctgccc cgtgccgtcg gactgatgaa
    13321 ggcgtcggcg tggatggaca cgaacaggtc cgcctgcacc cgccgcgcct tgctgacccg
    13381 ttgctgcagc ggcacgaaat agtccgcgtc tcgggtgagc atggcccgca tgttgggctc
    13441 cgcgtcaatg cgcgccttga ggcgccggcc gatggacagg gtgacgtgct tctcgaagct
    13501 gccggcggcg cccacggcac ccgggtcttc gccgccgtgg ccagggtcga gggcgatcgt
    13561 gacgaggcgg ctcacgccct cgtcttcggc aggcttgcgc cgtgccgcgg ggggttccgg
    13621 cgcaggctgc gggcggaccg gcgggggctc cgcctcctgc cggcgggcga cgccgggttc
    13681 ggcggggcgc gtcgggcgca cgctggacgg ttcgggccgc gcaggcatcg gcacgcctgc
    13741 cgccgcctct tcgggcgaga gcttctccag cagggcgagg aggggatcag ggggttcgac
    13801 gggatagaga tccagcacca ggcggtgacc gtattcgccc accggcttga gggtgaagac
    13861 ctgaggcacg atctccgcct tcagatcaat gacgaggcgc actacgccag gccggttgcg
    13921 gccggcgcgg atcagctgga tgtatggatc ctggccggag atcttggtcg gcagggattg
    13981 cagcacgcca ttgagttcga tgccctccag atccacgacc aggcgttccg gtccgtgcac
    14041 gagctgatgg gtaaatgtga gcggttcgcg gctttccagc gtgatgcggg tgtagtcctg
    14101 ggccggccag acccgcacgg ccaggatgcc ggtctgggcg gcagtgccgg gacgactcac
    14161 cagcagggcc agacttgcgc cggcggcgcc gatgaagcgg cggcgggtaa agcccgctgc
    14221 gtttccgcca atcgatgggc gggtcaggcg cgcggggaag gtgagaggcg atcgacgcat
    14281 gtccgtccct ccggggtcag cgcttggaga tccagcgtac ggccaccgtc gtccgcaatc
    14341 cgtagccgga tcgccagatc agcggggggc agataggggc ctgcccgctc gggccattcg
    14401 acaaggcgta agccgggccc ttgaaaatac tcgtccagtc cggcttcgag gaactcttct
    14461 gcatgggtga atcgataaaa atcaaagtga tatacgacaa ttctagaatc tttatacgat
    14521 tcaaccaagg tgtaagtggg acttttgacg cggccggtgt agtccagtcc gcggagcagg
    14581 ccgcgcgtaa gcgtcgtctt gcccgcgccc agatcgccgt gcaggaggat gatcagccgc
    14641 tccggcatga cggatgcgag ggtgcgaccg taggtcaggg tggcctcttc atccgccagg
    14701 tgcgcgcaaa gcacagggtg tttatcatgg gcgtcatgga cacagggcac gatgtactct
    14761 cacggaaggc tgaggatctc gccgcactcg cgacgcgcat ccgggcgtgg ggggaagaac
    14821 tgggctttgg cggcatcggc atttccgatg tcgcgcttga gggcgtggaa gagggactgg
    14881 ctgcatggtt ggctgccggt ttccacggat ccatggatta tatggagaaa cacgggacca
    14941 agcggacgcg accggcggaa ctcgtggcgg gcacgcggcg cgtcatctcg gtgaggatgg
    15001 attatctgtc ggcggctgcg gcccatgcct gcgaggtgct ggcggatcgt agtctggcct
    15061 acgtttcacg gtatgccctg gggcgggact atcacaaggt gctgcgccag cggctgcaga
    15121 agcttgcgga ccggatctcc gccgcagcgc ctcacggcta ccgggtgttc accgattccg
    15181 cgcccgtgat ggaggtggca ctggcggaaa aggcaggttt agggtggcgg ggaaagcata
    15241 gcctgttact atcgcgtgaa gcggggtcat tcttttttct gggagagatc tatactgatc
    15301 tggaattgcc ggtggacgcg cctcgcagtg gacattgcgg ttcctgccgg gcatgcatcg
    15361 acgcctgccc gacgggtgcg atcgtggcac cctatcgggt ggatgcgcgg cggtgcatct
    15421 cctatctcac catcgagctg cacggtgcga tcccggaggt ttatcgcccg atgatcggca
    15481 accgtgtgta tggctgcgac gactgccagt ggatatgccc ttggaaccgc tttgcaggac
    15541 aggcgagtgt gtcggatttc acgcctcgaa atgggcttga tcgcataaaa ctgttagagt
    15601 tgtttaattg gacgccacag attttcaacg aacggatggc gggtagtccc attttccgta
    15661 ttggatatga acgttggatg cgtaacctcg ccgtcggact gggcaacgcg ccctcatccg
    15721 cagaggtggt tcgcgcgctt caatcgcgac gcgatgatca atcctggctg gtccgggagc
    15781 atgttgcatg ggcgctcgag aggcacggtg cgctgagatg cgggatgtcc tggcaatgat
    15841 gtcgcgcaag ccggcgccgg aggaggaacg tccgccgcag acgctcgcgg aagtgactgc
    15901 catggagtcg tggcgcttcg accccctgtg catcgcggag gacgatccca aggtctcggc
    15961 gccgtcggac agcagcaaca agttccgcat caaaatgctc atgcgggacg gtgaccgccg
    16021 ccgtgagacc gatctgctca tcgaaaagcg ctacgcgtgg cgcgggtatg gcaagctcgg
    16081 gtcccttgaa gcgccgaacc agctcacgat gaacgcggag ctcttcgggc gcgtctatgc
    16141 gaccctgacg gtcaatatcg attccccggc gggtctggcc ctcgacaaga cctatggcgc
    16201 ggaagccgcc cggctgcgtt cccaggggcg caagctgtgc gagttcgggc gcttcgcagt
    16261 ggatccctcg gcgcgttcca agcggctcat cgcctcactg ttcaatctgc tctacatcta
    16321 ctcgtaccgg gtcaaggggt gcaccgacat cctcatcgag atcaatccgc ggcaccggga
    16381 tttctacgag aagctcctcg aattccacca gctcggcgaa gtcaagcact gcgcccgtgt
    16441 caatgccccg gccatgctga tgcatctgtc ctgcgccacc atcgaagccc gcctgaagga
    16501 gatcggcggg cgctggcgcg agttgcccga tgagaaatcc atctacaagt tcgcgttcac
    16561 gcccgaggaa gagtccgaac tgatcggccg cctcgacgac gaccactgat ccgcccgtcc
    16621 cgcagtccgc cgatctggcg gtgactcgcg taaaatcccg cttccctcgt ttgcgccgcg
    16681 cccgcggcac ccgccatgtc tgaaaccggc acgctgccca tcggagtctt cgattccggc
    16741 gtgggcgggc tcaccgtcgt acgcgccctg atggagcgcc tgcccctcga aagcatcgtc
    16801 tatttcggcg acaccgcgcg ggtgccttac ggcgtcaagt cagtggccac catcgaacac
    16861 ttcaccgggc agatcaccga ccacctgctg caccagggcg tcaagatgct gatcatcgcc
    16921 tgcaacacga tggcggcggt ggcgtcccac gtggtgaagg acaaggcggg cgatatgccg
    16981 gtgctggacg taatcgatgc gggcgcgcgg gccgccgtgg cggaatcccg ctcccgcgcc
    17041 atcggcgtga tcggcacccc caccaccatc aacagcaatg cctacgcccg gcgcatgcat
    17101 gcccttgacc caaacgtaag ggtctattcc caggcctgtc cgctgttcgt gccgctggtg
    17161 gaagagggct ggctggagca cgaagtgacg cgcctcaccg cccaggaata cctgcgcccg
    17221 gtgctggccg agaacgtgga tacgctggtg ctcggctgta cccactatcc cctcatcaag
    17281 cccctgctgc aggacgtggt ggggccgcag gtgcgcctcg tggattcggc catcacggtg
    17341 gccgagcagg ccgcgcgcgc cctcgcggac ctgggtatcg ccaatccggg cgaccggctg
    17401 cccacatatc gttacacggt caccgacatt cctttgcgtt tccagaccat cggtgagcgc
    17461 tttctcggcc gctccctggg tgtcgtggac aaggtggact ggtaggcgca tcgtccgcag
    17521 cagggcgccg atcgaacccg cgaccgtcgg ccgggattcg aaagcgggac gcccgcggga
    17581 tgacgcgtcc tgcaggttca ggcgttcttg cgcttctgcc agagcacgtc accgcgtccg
    17641 ccggccttgt tgagcacccg gcccagcacg aagagcaggt ccgacagtcg gttgaggtac
    17701 tgacgcggcg cgtcattgac ggcctcttcc tgggccagcg cgacgatcat ccgctccgcg
    17761 cgccggcaca cggtgcgggc ctggtggcag agggccgcgg cgcgggtgcc gccgggaagg
    17821 atgaagtcct tgagcggctc cagatcgtcg ttgtggcgat ccagttcgtc ttccaggtac
    17881 tgcacctgct ttgcagtgat catctccatg cccggaatgc agacctcgcc gcccaggtcg
    17941 aagaggtcgt gctgcacgtt ggtgagcagc acgcgcacgt cctccggcag gtcctcgcag
    18001 aggagcacgc cgacggcggc attgacttca tccacctcgc ccagggtgtg gatgcgcagg
    18061 ctgttcttgg atacccggtt gccgtttccc aggccggtgg tgccggcatc gccggtgcgg
    18121 gtgtagatct tggacaggcg gtggcccatg gctgtgtctc cctcggagtc gatgttatgg
    18181 ttgttcccgg cagggccggg caggggatta tagccagccg ggcctcgcgg ggaggaatgc
    18241 tacaatccgc cgtttagaaa cccgcgttcc cccgtgcgtc gcccggcgac gccctccgga
    18301 gactccccgc cgatgaaaag cgccgaaatc cgtcagaagt tcctcgaatt cttcgcctcc
    18361 aagggccacc agatcgtcgc ctccagctcg ctggtgcccc acgacgaccc gaccctgctg
    18421 ttcaccaacg cgggcatgaa ccagttcaag gacgtcttcc tcggcttcga caagcgcccc
    18481 tacacccgcg ccaccacgtc ccagaagtgc gtgcgcgccg gcggcaagca caacgacctg
    18541 gagaatgtgg gctataccgc ccggcatcac accttcttcg agatgttggg caacttcagc
    18601 ttcggcgact actttaagcg tgacgcgatc cattacgcgt gggagctgct caccgaggtc
    18661 ttcaagctgc ccaaggacaa gctcaccgtc accgtgtatg ccgaggacga cgaggcctac
    18721 gacatctgga cgaaggaagt cggcgtgccg gccgacaagg tcatccgcat tggcgacaac
    18781 aaaggcgcgc gctacgcctc cgacaacttc tggatgatgg gcgacaccgg tccctgcggc
    18841 ccgtgcaccg agatcttcta cgaccacggc gcgcacatct ggggcggccc gccgggatcg
    18901 cccgaggagg atggcgaccg tttcatcgag atctggaaca acgtgttcat gcagttcaac
    18961 cgggacgagg ccggagtgat gcacccgctg cccaagccca gcgtggacac cggcatgggc
    19021 ctggagcgca tctcggccgt gctgcagggc gtgcacgcca actacgagat cgacctgttc
    19081 cagaccctgc tgaaggctgc cgcccgcgaa acctccgcgg cggatctgga cagcccgtcc
    19141 ctcaaggtgc tggccgacca catccgtgcc tgttccttcc tgatcgccga cggcatcatt
    19201 cccggcaacg aagggcgcgg ttacgtgctg cgccggatca tccgccgcgc catccgccac
    19261 ggctacaagc tgggcgtccg cgcggccttc ttccaccgcc tggtgcccga tctggtggcc
    19321 gagatgggcg acgcctatcc cgaactgaag agccaccagc agaaggtgac ggacgtgctc
    19381 aagcaggagg aagagcgctt cttcgccacc atcgagcacg gcatggccat cctcgaagcc
    19441 gacctcgccg agatgtccaa ggcgggcagc accgtgttca acggcgacac cgccttcaag
    19501 ctgcacgaca cctacggctt cccgctggac ctcacggccg acgtgtgccg cgagcacggc
    19561 gtgaccgtcg acaacgccgc cttcgacgcc gccatggcgc gccagaagga gcaggc
//
LOCUS       DAOQPA010000064        19577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1385_length_19577_cov_19.546973, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000064 DAOQPA010000000
VERSION     DAOQPA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1385_length_19577_cov_19.546973"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="ureC"
                     /locus_tag="PKX01_10195"
     CDS             <1..1277
                     /gene="ureC"
                     /locus_tag="PKX01_10195"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HMZ76540.1"
                     /translation="IEEALNSGVTTMIGGGTGPATGTFATTCTPGPWHIHRMLEAAEA
                     FPMNLGFLGKGNASLPEALREQVEAGVIGLKLHEDWGTTPAAIDCCLSVADEMDVQVA
                     IHTDTLNESGFVEATIDAFKGRTIHTFHTEGAGGGHAPDIIKAAGLPNVLPSSTNPTM
                     PYTVNTIDEHLDMLMVCHHLDPAIAEDIAFAESRIRRETIAAEDILHDLGVFSMMSSD
                     SQAMGRVGEVIIRTWQAAHKMKVQRGTLPEDCTRHDNFRVKRYIAKYTINPALTHGIA
                     HTVGSVEVGKLADLVLWRPAFFGVKPSLILKGGMIAAAAMGDPNASIPTPQPVHYRPM
                     FGSFGKALKTSVTFVSQAALKNPAVAALGLSKPLVAVSGTRTLSKADMVHNGATPEIT
                     VDPETYVVKADGVHLVCEPATELPLAQRYFLF"
     gene            1291..1569
                     /locus_tag="PKX01_10200"
     CDS             1291..1569
                     /locus_tag="PKX01_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HMZ76541.1"
                     /translation="MTDPIARLANLGPKSSAMLAAAGIATVEDLRALGAVAAYARVKA
                     RDPHASLNLLWALEGALSGLPWQTVAREHRTSLLLALETCQTSPAPEC"
     gene            1563..2117
                     /gene="ureE"
                     /locus_tag="PKX01_10205"
     CDS             1563..2117
                     /gene="ureE"
                     /locus_tag="PKX01_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HMZ76542.1"
                     /translation="MLNLTQRTNAPATDAVALAYDDRKRSRLKVTLASGREAGIFLER
                     GEHLQGGDRLAGDDGATVVEILAAPEQLIEAEAGTPLLFARAAYHLGNRHVPVQLLPT
                     ASGGVLRFQTDHVLAEMVKGLGCTVAETDAPFQPEAGAYGAHGGHQHPYHPGEAGATG
                     QADLHDPGHGPHRSVPKIHQFRAR"
     gene            2120..2326
                     /locus_tag="PKX01_10210"
     CDS             2120..2326
                     /locus_tag="PKX01_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HMZ76543.1"
                     /translation="MPYVNVQITRGATREQKAEIVRDITATLVRVLGKRPEHTHVVLQ
                     EIAEEDWGYAGLLTDEWKRQQGGA"
     gene            2326..2766
                     /locus_tag="PKX01_10215"
     CDS             2326..2766
                     /locus_tag="PKX01_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76544.1"
                     /translation="MRTGTLLRQTPLLVALLASSAPGAAVTPAYRGPDLSGTYVCTGH
                     DHQDGPYTGTVTLALLRTRSTGAQASYDFRLDVPGYGSYPGHAAAQGRHMAISFANTD
                     PTTHDYGTGIARFHRQHGKWAFDKFYHEPEYKGGNFGTEHCVQR"
     gene            2854..3540
                     /locus_tag="PKX01_10220"
     CDS             2854..3540
                     /locus_tag="PKX01_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HMZ76545.1"
                     /translation="MNDTLALARLLQLASPALPVGAYTYSQGLEWAVESGVIRDEAGA
                     ARWIGDLLAHAIGRFEAPLLARQMQAWRDTDHTEVLRLDADFLASRESAELRAETAQM
                     GFSLRRLLRDLRDEGLAPVALVVDPLAEVAFPTVWSGIAATWGIAPHAAVVAYLWSWA
                     ENQVMAALKAVPLGQAAGQRLLATLGTRLPALADAALDLPEAAWSNFTPAFALACARH
                     ETQYSRLFRS"
     gene            3543..4151
                     /gene="ureG"
                     /locus_tag="PKX01_10225"
     CDS             3543..4151
                     /gene="ureG"
                     /locus_tag="PKX01_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HMZ76546.1"
                     /translation="MTQQALRVGIGGPVGSGKTALTLALCQALREQIDMAVVTNDIYT
                     AEDAQFLVRHAALPPERIIGVETGGCPHTAIREDASINLEAVDRLQRAFPDLRLILVE
                     SGGDNLAATFSPELSDLTLYVIDVAAGEKIPRKGGPGITKSDLLVINKIDLAPLVGAS
                     LDVMAHDARAQRGERPFVFTNLKSGEGLDIVIRFIRDRGMLD"
     gene            4256..5749
                     /locus_tag="PKX01_10230"
     CDS             4256..5749
                     /locus_tag="PKX01_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76547.1"
                     /translation="MSASSPSLSERSAPILDWLASETSEDTDLEISALVRHLEEMAGS
                     EINRNQFYRCLELIHTRSLVIAAQMRQHLATGALPLTIGLHRSATRMIGALGKIVDGY
                     EQLLEETRNRLVRTTRRNPETLSAKALRLLGEQLTLSHLAGSAAPFDFWQRAHALFRL
                     SGRDAQTGDGAAEAPESVATAYKYLLCLAATQPEGLTGREICWLSDYIAEHANECRLR
                     DTAPTRNRAAWYWIDPDQNASPVSFSRREPPPVDGLLYFSAEELARGATELIDTLDGD
                     QAPTHDLPAWLRRGEAATLLRRVREYWAAPPHRAHTRRRNQYPITVCCGLESIWKMLR
                     APAGPRQNRFSTEWMVVNESPTGYAIMQVTGAAANLGPGIALALRRSEDEQWTICVVR
                     WIRTETPEQVELGLQVVANSAKPVTVGFRNSAKAQPMKPALVLPPLPALGKAQSILAP
                     GGTYTARRFMLVSDTQRLYVAQGRLLSLDMQTAAVELFQYEIDPYPL"
     gene            complement(5737..7005)
                     /locus_tag="PKX01_10235"
     CDS             complement(5737..7005)
                     /locus_tag="PKX01_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HMZ76548.1"
                     /translation="MITDTHSLREGRLTAEAARHLAVAVWRRFHEGRCAQVAGSLTFT
                     SLLSLVPFVTLVVLTFSRFPQSTQFSEALKGFLLDNLLPDKAGKVIAAYAVQFSQKST
                     NLTIVGGVVLVVTALSLIKTIDQAINQIWNVRGTRRWATRFAVYWAALSVGPVFLAGC
                     VFASSAVLSLSLDLVGEPVWLGAVALRLMPVALLTGLFAAVYYIVPHCTVRPVDALAA
                     GFLAALLLVSMQRLFGLYLTHFTSYTLVYGAFAVVPIFLMWLYLSWIVILLGAVVAAV
                     LPERTLLGRMLPAFPGRSLYLALLVLGQLVRAQRRGLTPDVGHLARTARAGHDEVRAV
                     LESCQRARIVVRSDEGGWLLARAADAVSLAEVAQLFAWDGVPRKDAVATPLEAQIRPL
                     WETWVARLNVASELPLSVLAEAGDAKDQSG"
     gene            7042..7638
                     /gene="wrbA"
                     /locus_tag="PKX01_10240"
     CDS             7042..7638
                     /gene="wrbA"
                     /locus_tag="PKX01_10240"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HMZ76549.1"
                     /translation="MHEILVLYYSHRGAVRALAELIARGIDAVPGVAARLRTVPRVST
                     VCEATEGDIPERGAPYVEIRDLEECIGLALGSPSRFGNMAAPMKYFLDGTAGPWLSGA
                     LAGKPACVFTSSASPHGGQESTLLSMMIPLLHHGLLLVGLPFSEPDLTRTRTGGSPYG
                     ATHVAGPDDSNPISDEEKRLAVALGRRLAETALKLVAR"
     gene            7635..8003
                     /locus_tag="PKX01_10245"
     CDS             7635..8003
                     /locus_tag="PKX01_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HMZ76550.1"
                     /translation="MKPKTLSLIASLTLVTLIALCLAWETVLAPVRPGGSWMTLKVLP
                     LMAALFGILRGTRYTYQWSSMMILLYVTEGVVRASDPAPGGQLALAEAVLSIVFFVAV
                     VLYARNTAPSRLARAAQQDA"
     gene            complement(8037..9569)
                     /locus_tag="PKX01_10250"
     CDS             complement(8037..9569)
                     /locus_tag="PKX01_10250"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMZ76551.1"
                     /translation="MNDQLIIFDTTLRDGEQSPGASMTREEKLRIARQLERMKVNVIE
                     AGFAAASNGDFEAVKAVAEAIKDSTVCSLARANENDIRRAGEAIRPARSGRIHTFIAT
                     SPIHMEKKLRMSPDEVVAQAVKAIGWAREYTDDIEFSAEDAGRSELDFLCRIFEAVIR
                     AGATTINVPDTVGYNVPEQFAATIRSLIERVPGSDKVVWSVHCHNDLGLAVANSLAAV
                     RAGARQVECTVNGLGERAGNASLEEVVMAVRTRKDIFLCDSRIDTTQIVPASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWTANKLVLGKHSGR
                     NAFRSRLQELGIVIGSEEQLNHAFARFKELADKKHEIYDEDLQALLSDESYEPKEHFR
                     LVSSSFHSETGETPCAKLTLSVGGKEANSESCGSGPVDAAFKAIEVIADSGTQLLLYS
                     VNAITTGTDAQGEVTVRLSKDGKIVNGQGADTDIIVASAKAYLNALNKLHAKLERLNP
                     QV"
     gene            complement(9768..10550)
                     /gene="pssA"
                     /locus_tag="PKX01_10255"
     CDS             complement(9768..10550)
                     /gene="pssA"
                     /locus_tag="PKX01_10255"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HMZ76552.1"
                     /translation="MADPYARKPLLNPERRRRGIYILPNLFTTAALFAGFYAIVQAMN
                     GQFEQAAVAIFVAMVMDGLDGRVARLTRTQSAFGAEYDSLADMVSFGAAPSLIVYEWA
                     LKGMGKLGWIAAFIYCAGAALRLARFNTNIDVIDKRYFQGLPSPAAAALVAGLVWVVI
                     DNGLSGQDVRWVACTLTIFAGITMVSNVLFYSGKEINLRRSVPFFVVIAFVAGFALVS
                     SYPQGVLFGLFVCYAASGYVFAAWRWLGRRKAAVAGAEKSDL"
     gene            complement(10696..11712)
                     /gene="ilvC"
                     /locus_tag="PKX01_10260"
     CDS             complement(10696..11712)
                     /gene="ilvC"
                     /locus_tag="PKX01_10260"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMZ76553.1"
                     /translation="MKVYYDKDADLSLIKGKQVTIVGYGSQGHAHAQNLRDSGVNVTV
                     GLRKGGASWSKAEGAGLKVAEVAEAVRAADVVMILLPDENIPEVYRNDVEPNIKQGGV
                     LAFAHGFNVHYNQVVPRADLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVYQDKSGKA
                     RDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEFGEYVTGTEVINEQSRAAMRN
                     ALKRIQTGDYAKMFILEGRTNYPAMTARRRLNAVHPIETVGAQLRDMMPWIKANKLVD
                     QSKN"
     gene            complement(11761..12252)
                     /gene="ilvN"
                     /locus_tag="PKX01_10265"
     CDS             complement(11761..12252)
                     /gene="ilvN"
                     /locus_tag="PKX01_10265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMZ76554.1"
                     /translation="MRHVISLLIENESGALSRVSGLFSARGYNIESLTVAPTEDASVS
                     RMTIVTSGSDEIVEQITKQLNKLIEVVKVVDLSESAHIERELMLVKVRATGKDRDEMK
                     RLSDIFRGRIIDVTESTYVIELTGTQAKLDSFVGAIDPALILETVRSGICGVGRGDRV
                     LKV"
     gene            complement(12353..14089)
                     /locus_tag="PKX01_10270"
     CDS             complement(12353..14089)
                     /locus_tag="PKX01_10270"
                     /inference="COORDINATES: protein motif:HMM:NF005409.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HMZ76555.1"
                     /translation="MAETASGAAMVLTGAEIVIRCLQEEKVDYLFGYPGGAVLYIYDE
                     IFKQDKVRHVLVRHEQAAIHAADGYARSTDRVGVALVTSGPGATNAVTGIATAYCDSI
                     PMVVISGQVPSAAIGQDAFQEVDTVGITRPCVKHNFLVRDVREIAPTLKKAFYLARTG
                     RPGPVLVDIPKDITAQKCEFVYPASVSMRSYNPVVKGHHGQIRKALQLLLEAKRPMVY
                     AGGGVVLGDASEKLTNLVRRLGFPCTNTLMGLGGYPATDRQCLGMLGMHGTYEANMGM
                     HYCDVLLAVGARFDDRVIGNPDHFASEPRKIIHIDIDPSSISKRVKVDVPIVGDVGEV
                     LGELLALLEGTSERPDAEALDVWWKQIEEWRRRDCLKFTQPDDIIKPQFVVQKLWEAT
                     GGDAFVTSDVGQHQMWGAQYYKFDKPRRWINSGGLGTMGFGMPAAMGVQLAHPGSPVA
                     CITGEASIQMNIQELATCKQFRLPIKVVMLNNRYLGMVRQWQEIFHGNRYSESYMDSL
                     PDFVKLAEAYGHVGIRVDKPADVEGALREAFQRKNDLVFLDFQVDPTENVYPMVQGGK
                     GLTDMILSAEDL"
     gene            14178..14786
                     /locus_tag="PKX01_10275"
     CDS             14178..14786
                     /locus_tag="PKX01_10275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ76556.1"
                     /translation="MSGYSSASPARNSPLSSRIELSDFLASVEKRAFKQALFAVRNEE
                     TALDIVQDAMMKLAEKYGDRPPEELPMLFQRILQNVIRDHYRRQKVRSMWTTLLSAFS
                     GPDEEDEHDPLETLESADSEFRSPSPQAEVEQQQTLSLIEREIEKLPPRQREAFLMRY
                     WEEMDIAETAAAMGCSEGSVKTHCSRATHALAAALAAKGITL"
     gene            14783..15154
                     /locus_tag="PKX01_10280"
     CDS             14783..15154
                     /locus_tag="PKX01_10280"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HMZ76557.1"
                     /translation="MIEHEIHRRLRQHLNRSLQRIEPDTSERLHAARRKALSRQKVAV
                     HGLSLAGAGRLITDHLPDRRTAVAMVLVVVIALASGFLGDLQRTADIEEVDSSLLADD
                     LPIDAYLDRGFDTWVQSQALE"
     gene            15127..15747
                     /locus_tag="PKX01_10285"
     CDS             15127..15747
                     /locus_tag="PKX01_10285"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HMZ76558.1"
                     /translation="MGAKPSSRVIAALALCWAAHAAAVPPLKAPSWNELTPAQQRTLA
                     PLVKEWSNLPDDRKRKWVGIADRYPGLTPEEQARLQQRMHEWASLTPAQRESAREQYR
                     NLQKMPPEQRQALREKWEAYLELPEEEKKRFTDAAPKPGPKPPVPGQPATPIKPLTAP
                     PQPARPVVLPKPVPPPAPLTAPAPVPVPVPAQAPVVAGQDPTPPKP"
     gene            15751..16221
                     /locus_tag="PKX01_10290"
     CDS             15751..16221
                     /locus_tag="PKX01_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ76559.1"
                     /translation="MMPSTATSGATPRPTGELATLRRRLASMLYEVLLLLGVLALTFL
                     VPLLILGIGFGILPPGGVMWLWIYLALGGYFVWYWRQGGQTLAMQTWKLRIVAANGDT
                     VSVRQAWLRYSLAWASLCVGGLGLFWALIDPERQFLHDRLAGTRIVLLLAPPAD"
     gene            complement(16229..17305)
                     /gene="lptG"
                     /locus_tag="PKX01_10295"
     CDS             complement(16229..17305)
                     /gene="lptG"
                     /locus_tag="PKX01_10295"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HMZ76560.1"
                     /translation="MAPIFVRYLAREIYAAVAMVLLAFLGLFAFFDLVNELDDVGKNG
                     YMLYHAALYVAMILPGRVYELMPIAVLIGTLYALTTLARHSEITVLRASGLSTGRFLA
                     ALLTIGSVFVAITFVFGEYLAPPAERAAQQWRLAATRSMVSQELRTGLWVRDGHRFVN
                     VRNVMPDTTLEGVRLYEFDDRHALVSISEATRGVFDERNGWRLSEVAQTRFLGDRTET
                     VSLPELVWHSELTPDVLSVLMVVPERMSVTTLYSYIRHLEDNNQRTTRYEIALWKKLV
                     YPFAALVMMGLALPFAYMQNRMGGVSLKVFSGIMIGVGFHLLNGLFSNLGVINGWVPA
                     VAALTPSVTFLVAAATMMWWVERR"
     gene            complement(17305..18381)
                     /gene="lptF"
                     /locus_tag="PKX01_10300"
     CDS             complement(17305..18381)
                     /gene="lptF"
                     /locus_tag="PKX01_10300"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HMZ76561.1"
                     /translation="MIFHRAAQREFTQNAVAVFVALFAILISTVLIRLLGQAAGGRVP
                     ADAVLALIGFGAIAQMPVLLSLTVFIAILMSLSRAYRDSEMVVWFSCGLPLTAWVRPV
                     LRFALPVVIVVALLSLFGAPWAQKKSVEYKEQLNNRDDVSRVAPGMFRESSGGERVFF
                     VEAASGEEGRVRNVFASSMQNGRLGVMVAANGHSEVDGLGNRFVILEHGRRYEGVPGT
                     PSYRVMEFERYQIRMQSKEAVTGPRLPKTLTFPALLKEKGNLYRGELLARLSTPVLAV
                     MLALLAIPLSFVNPRAGRTNNLILAILVYLIYSNALSVSQAWVAQGRLGFEVALMAPH
                     VVMMLLLAVLFWKRLSVFARWRRR"
     gene            18508..>19577
                     /locus_tag="PKX01_10305"
     CDS             18508..>19577
                     /locus_tag="PKX01_10305"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HMZ76562.1"
                     /translation="MEFTIKAGSPEKLKSGCVVAGVFAGGELSAAAKTLDKASSGALA
                     TILARGDLDDKAGATLMLHGVPGIEAPRVLLVSLGAASELTDKRYRDAVAAAARQLAA
                     SMAGDAAFCLAEADVPGRDLAWRIAQAAQILQDSTYRCDELKSRKEDSKKGVVHVAFI
                     TRAKHGDELQAALRRGEAIAEGLALAKTLGNLPGNVCTPGYLATTAKKLAKEHKIKVE
                     ILDRDAMEKLGMGALLSVARGSHEAPRFIILQYKGGKAKDKPVVLVGKGITFDTGGIS
                     LKPGEGMDEMKYDMCGAASVLGTFKAIARMELPLNVIGLIPSTENMPGGSATKPGDIV
                     TSMSGQTIEVLNTDAEGRLILC"
BASE COUNT         3464 a         6901 c         6131 g         3081 t
ORIGIN      
        1 agatcgagga ggcgctcaat tccggcgtca ccaccatgat cggcggcggc accgggccgg
       61 ccaccggcac gtttgccacc acctgcacgc cggggccgtg gcacatccac cgcatgcttg
      121 aagcggctga ggccttcccg atgaatctcg gcttcctcgg caagggcaac gccagcctgc
      181 cggaagcgct gcgcgagcag gtcgaagccg gcgtcatcgg tctcaagctg cacgaggact
      241 ggggcaccac gccggcagcc atcgactgct gcctgtcggt cgccgacgag atggacgtgc
      301 aggtcgccat ccacaccgac acgctcaacg aatccggctt cgtcgaggcg acgattgacg
      361 ccttcaaggg ccgcaccatc cacaccttcc acaccgaagg cgccggcggc ggccacgcgc
      421 cggacatcat caaggcggcc gggctgccca acgtgctgcc cagctccacc aacccgacca
      481 tgccttacac ggtgaacacc atcgacgagc atctcgacat gctgatggtg tgccaccacc
      541 tggatccggc catcgccgag gacatcgctt ttgccgaatc gcgcatccgc cgcgagacca
      601 tcgcagccga agacatcctc cacgacctgg gcgtgttctc gatgatgtcg tcggattccc
      661 aggccatggg ccgcgtcggc gaggtgatca tccgcacctg gcaggccgcc cacaagatga
      721 aggtccagcg cggcacgctg cccgaggact gcacgcgcca cgacaacttc cgcgtcaagc
      781 gctacatcgc caaatacacg atcaacccgg ccctgaccca cggcatcgcc cacaccgtcg
      841 gctccgtcga agtgggcaag ctggccgacc tggtgctgtg gcgaccggcc ttcttcggtg
      901 tcaaaccgtc gctgatcctg aagggcggca tgatcgcagc cgccgccatg ggcgacccga
      961 acgcgtccat cccgacgccc cagcccgtgc attaccggcc catgttcggc agcttcggca
     1021 aggcgctgaa aacctcggtg accttcgtgt cccaggccgc gttgaagaac cccgccgttg
     1081 ccgccctggg cctgagcaag ccgctggtgg ccgtctccgg cacccgcacg ctgagcaagg
     1141 ccgacatggt gcataacggc gccacgcccg agatcaccgt cgatcccgaa acctatgtgg
     1201 tgaaggccga cggtgtgcat ctggtgtgcg aaccggccac cgagctgccc ctggcgcagc
     1261 gttacttcct gttctgaagc cgtccgcgcc atgaccgacc cgatcgcccg cctcgccaac
     1321 ctgggaccga agtcctcggc catgctggcg gcggcgggca tcgccactgt agaggatctg
     1381 cgcgccctcg gcgcggtggc agcctacgcc cgcgtcaagg cccgcgaccc gcacgccagc
     1441 ctcaacctgc tgtgggccct cgaaggcgcc ctcagcggcc tgccctggca gaccgtggcg
     1501 cgggaacacc gcaccagcct cctgctggcc ctggaaacct gtcaaacatc gcccgccccc
     1561 gaatgctgaa cctcacgcaa cgcacgaacg cccccgccac cgatgccgtg gccctcgcct
     1621 acgacgaccg caaacgcagc cgcctcaagg tgacgctcgc ctccggccgc gaggccggca
     1681 tctttctcga gcggggcgag cacctccagg gcggcgaccg cctggccggc gacgacggcg
     1741 ccaccgtcgt ggagatcctc gccgcgccgg agcagctcat cgaagccgag gccgggacgc
     1801 ccctgctctt cgcccgcgcc gcctatcacc tgggcaaccg ccacgtgccg gtgcagctcc
     1861 tgcccaccgc cagcggcggc gtactgcgct tccagaccga ccacgtgctg gccgagatgg
     1921 tgaagggcct gggctgtacc gtggccgaga ccgacgcccc cttccagccc gaggccggcg
     1981 cttacggcgc ccacggcggc catcagcatc cctaccatcc cggcgaggcg ggcgccaccg
     2041 ggcaggccga cctccacgac cccggccacg gcccgcaccg ctccgtccca aagatccacc
     2101 agttccgggc ccgctgagca tgccctacgt caacgtccag atcacccgcg gcgccacgcg
     2161 ggaacagaag gccgagatcg tgcgcgacat caccgccacc ctggtgcgcg tcctcggcaa
     2221 gcggccggag cacacccacg tcgtcctcca ggagatcgcc gaggaggact ggggctacgc
     2281 cggtctgctc accgacgagt ggaaacgtca gcagggaggc gcctgatgcg caccggtacc
     2341 ctgctgcgtc aaacgccgct cctcgtcgcc ctgctcgcca gttccgcgcc gggtgccgcc
     2401 gtgacacccg cctaccgcgg tccggacctc agcggcacct atgtctgcac cggccacgac
     2461 catcaggacg gaccctatac cggcacggtg accctcgccc tgctgcgcac gcgcagcacc
     2521 ggcgcccagg ccagctacga cttccgcctc gacgtgcccg gttacggcag ctatcccggc
     2581 cacgccgcgg cccaggggcg ccatatggcc atcagcttcg ccaacaccga cccgacgacc
     2641 cacgactacg gcaccggcat cgcccgcttc caccgccagc atggcaaatg ggccttcgac
     2701 aagttctacc atgagcccga atacaagggc ggcaacttcg gcaccgagca ctgcgtgcag
     2761 cgctgagatg gaacgcccgc ccccctcact ccgttcgctg tccccctgtg caggtgcaag
     2821 cctcccttcg aacggcccgt caggcggctt gagatgaacg acaccctggc cctcgcccgc
     2881 ctcctgcaac tggccagccc ggccctgccg gtgggcgcct acacctattc ccagggcctc
     2941 gagtgggcgg tggaatccgg cgtcatccgt gacgaagccg gcgccgcgcg ctggatcggc
     3001 gatctgctgg cccacgccat cggccgcttc gaggccccgc tgctggcccg ccagatgcag
     3061 gcctggcgcg acaccgatca caccgaagtc ctgcgcctcg acgcggattt cctggccagc
     3121 cgtgaaagcg ccgagctgcg cgccgagacg gcccagatgg gcttctccct gcgccgcctg
     3181 ctgcgcgacc tgcgggacga gggcctggcg ccggtggcgt tggtggtcga tcccctggcc
     3241 gaagtggcct ttcccacggt gtggagcggc atcgccgcca cctggggcat cgcgccccac
     3301 gcggccgtcg tcgcctacct gtggtcctgg gccgaaaacc aggtgatggc cgccctcaag
     3361 gcggtgcccc tggggcaggc cgccggccag cgcctgctcg ccactctggg cacgcgcctg
     3421 cccgctttgg ctgacgcggc cctcgatttg cccgaggccg cctggagcaa cttcacgccg
     3481 gccttcgccc tcgcctgcgc ccgtcacgaa acccagtatt cccgactctt cagatcctga
     3541 ccatgaccca gcaagccctc cgcgtcggca tcggcggccc cgtcggctcc ggcaagaccg
     3601 ccctcaccct cgccctgtgc caggccctgc gcgagcagat cgacatggct gtcgtcacca
     3661 acgacatcta caccgccgag gacgcccagt tcctcgtccg ccacgccgcc ctgccgcccg
     3721 agcgcatcat cggcgtggag accggcggct gtccccacac cgccatccgc gaggacgcct
     3781 ccatcaacct ggaggccgtg gaccgcctcc agcgcgcctt cccggatctg cgcctgatcc
     3841 tcgtggagtc cggcggcgac aatctggcgg ccaccttctc gccggaactc tccgacctca
     3901 ccctctacgt catcgacgtg gccgccggcg agaagatccc ccgcaaaggc gggccgggca
     3961 tcaccaagtc cgatctgctg gtcatcaaca agatcgatct ggcccctctg gtcggcgcct
     4021 ccctcgatgt gatggcccac gacgcccgcg cccagcgcgg cgaacggccc ttcgtcttca
     4081 ccaacctgaa gagcggcgaa gggcttgaca tcgtcatccg cttcatccgc gaccgcggca
     4141 tgctcgattg atatcgggcc gccgggcgtg accgacatcg tccacccgcc cggcggcccc
     4201 acgagcgcgg ggaagttcat tacaatggat gactggtccc gtcatatccc tgtcaatgag
     4261 cgccagcagc ccttccctct ccgaacgctc ggccccgatt ctggattggc tcgcctccga
     4321 gacgtcggag gacaccgatc tcgaaatctc cgccctggtc cgtcacctgg aggagatggc
     4381 cgggtcggaa atcaaccgga atcagttcta ccgctgcctt gagctgatcc acacccgcag
     4441 cctcgtgatc gccgcccaga tgcggcagca cctggcgacg ggcgccctgc ctctgaccat
     4501 cggcctgcac cggtccgcca cacgcatgat cggcgcgctc ggcaagatcg tggacggcta
     4561 cgagcaactg ctcgaagaaa cccgcaaccg cctcgtgcgc accacccggc gcaacccgga
     4621 aacgctcagc gccaaggcgc tgcgcctgct cggcgagcag ctcaccctct cccatctggc
     4681 gggcagcgca gcgcccttcg atttctggca gcgcgcccac gccctcttcc gcctgtcggg
     4741 ccgcgatgcc cagaccggcg acggcgccgc cgaagcgccg gaaagcgtcg ccaccgccta
     4801 caagtacctg ctgtgcctcg ccgccaccca acccgaaggg ctcaccggac gggaaatctg
     4861 ctggctgtcc gactacatcg ccgaacacgc caacgagtgt cgcctgcggg acacggcgcc
     4921 cactcgcaac cgggcggcat ggtactggat cgacccggac cagaacgcct cgccggtctc
     4981 gttttcgcga cgcgaaccgc ccccggtgga cggactgctg tacttctccg ccgaagagct
     5041 cgcccgcggg gccaccgaac tgatcgacac ccttgacggt gaccaggcgc ccacccacga
     5101 tctgccggcc tggctgcgcc ggggcgaggc cgccaccttg ctgcgccgcg tccgggaata
     5161 ctgggccgcg ccgccgcacc gggcccacac gcggcgccgc aaccagtacc ccatcaccgt
     5221 ctgctgcggc ctcgaatcca tctggaaaat gctgcgggcg ccagccggtc cccgccagaa
     5281 ccgcttctcc accgaatgga tggtggtgaa cgaaagcccc accggctacg ccatcatgca
     5341 ggtcaccggc gccgcggcca atctggggcc tggcatcgcg ctggccctgc gccgcagcga
     5401 agacgaacaa tggaccatct gcgtcgtgcg ctggatccgc accgagaccc ccgagcaggt
     5461 ggagctcggc ctgcaggtcg tggccaactc ggccaagccc gtgaccgtgg gtttccgcaa
     5521 cagcgccaag gcccagccga tgaaacctgc actggtgctg ccgccgctgc cggcccttgg
     5581 caaggcccag tccatcctcg cgccgggcgg cacctacact gcgcgccgct tcatgctggt
     5641 ctccgacacc cagcgccttt acgtggccca ggggcgcctg ctgagtctcg acatgcagac
     5701 cgccgccgtc gagctgttcc agtacgagat cgacccttat ccgctctgat ccttcgcatc
     5761 gccggcctcg gccaggacgc tgagcggcag ttcgctcgca acattaaggc gcgccaccca
     5821 ggtctcccac aggggacgga tctgcgcctc cagcggcgtc gccaccgcgt ctttccgcgg
     5881 cacgccatcc cacgcaaaca gctgcgccac ctccgccaga ctcaccgcat cggccgcccg
     5941 cgccagcagc cagccccctt catcgctgcg gaccacgatg cgcgcccgct ggcagctttc
     6001 aagcaccgcc cgcacctcgt cgtgaccggc ccgggccgtg cgcgccagat gccctacgtc
     6061 gggcgtgagg ccgcgccgct gggcccgcac cagctgcccc agtaccagca gcgcgagata
     6121 caaggaccgc cccgggaacg ccggcagcat gcgtccgagc agggtgcgct cgggcagcac
     6181 ggcagccacc acggcaccga gcaggatcac gatccaggac agatacagcc acatcaggaa
     6241 aatcggcacc acggcaaacg ccccgtacac cagcgtatag ctggtgaaat gggtcagata
     6301 cagcccaaac aggcgctgca tggagaccag cagcagcgcg gccaggaagc ccgccgcgag
     6361 cgcatcgacg gggcggacgg tgcagtgggg aacaatgtaa tacacggcgg cgaaaagccc
     6421 ggtgagcagc gccaccggca tcaggcgcag ggccactgcc cccagccaca ccggctcccc
     6481 caccagatcg agggacaggg acaggaccgc agaggacgca aacacacaac ctgccaggaa
     6541 gaccggtcca accgacaggg ccgcccagta caccgcgaag cgcgtggccc agcggcgtgt
     6601 accgcgcacg ttccagatct ggttgatggc ctgatcgatg gtcttgatga gcgacagggc
     6661 cgtcaccacc agcaccaccc cgccgacgat ggtcagattg gtggacttct ggctgaactg
     6721 caccgcatag gctgcaatta ccttccccgc cttgtccggc agcaggttgt ccagcaggaa
     6781 gcccttgagt gcctcgctga actgggtcga ctggggaaag cggctgaagg tcagcaccac
     6841 cagggtgacg aagggcacca gcgagagcag ggacgtgaag gtcaggctgc ccgccacctg
     6901 ggcacatcgc ccctcatgga aacgccgcca gacggccacg gcgagatggc gcgcagcctc
     6961 ggccgtcaat cggccctccc gcaaagaatg ggtgtcagtg atcatttccg tataatgccc
     7021 tccccacgtc cgggccacag catgcacgaa atcctcgttc tctattacag ccaccggggc
     7081 gccgtgcgcg ccctggcgga actcatcgcc cgcggtatcg acgccgtgcc cggcgtggcc
     7141 gcgcgtctgc gtaccgtgcc acgcgtctcc acggtctgcg aagccaccga aggggacatt
     7201 cccgaacgcg gcgcccccta cgtggagatc cgcgacctgg aggaatgcat cgggctcgcc
     7261 ctgggcagcc cgtcgcgctt cggcaacatg gcggcgccga tgaagtattt tctcgacggc
     7321 acggccggtc cgtggctgtc cggcgccctg gcgggcaagc cggcctgtgt attcacctct
     7381 tccgccagcc cccacggcgg gcaggaaagc accctgctgt cgatgatgat tcccttgctc
     7441 caccacggcc tgctgctggt cggcctgccc ttctccgagc ccgacctgac ccgcacccgc
     7501 acgggtggct ctccctatgg cgcgacccac gtcgccggcc cggacgactc caaccccatc
     7561 agcgacgagg aaaaacgcct ggccgtcgcc ctcggccgcc gcctcgccga aactgccctc
     7621 aaactggtcg cccgatgaaa ccgaaaaccc tgtccctgat cgccagcctg acccttgtca
     7681 ccctgatcgc cctgtgcctg gcctgggaaa ccgtgctcgc gcccgtgcgt ccaggcggtt
     7741 cctggatgac cctgaaggtg ctgcccctga tggcggcact gttcggcatc ctgcgcggca
     7801 cccgttacac ctatcagtgg tcgtcgatga tgatcctgct gtacgtcacc gaaggcgtgg
     7861 tgcgcgccag cgacccggcg ccgggcggtc agctggcgct ggccgaagcc gtgctgagca
     7921 tcgtgttctt cgtggccgtg gtgctgtacg cacgcaacac ggccccgtct cgcctcgccc
     7981 gcgccgctca gcaggacgcc tgacccgcag caccgggcgg cggacccggg cgctgctcag
     8041 acctgcggat tcagccgctc cagcttggcg tggagcttgt tgagggcgtt gaggtaggcc
     8101 ttcgcggagg cgacgatgat gtccgtgtcg gcgccctggc cgttcacgat cttgccgtcc
     8161 ttggacagac gcacggtcac ctcgccctgg gcatcggtgc cggtggtgat ggcgttcacc
     8221 gagtacagca ggagctgggt gccgctgtcg gcgatcacct cgatggcctt gaaggccgca
     8281 tccaccggcc cggagccaca ggattcggag ttggcctcct tgccgcccac cgacagggtc
     8341 agcttcgcgc agggcgtctc gccggtttcc gagtggaagg atgaggacac cagacggaag
     8401 tgctccttgg gctcatagct ttcatcgctg agcaaggcct ggaggtcttc gtcgtagatc
     8461 tcgtgcttct tgtccgccag ttccttgaaa cgggcgaagg cgtggttgag ctgctcttcc
     8521 gagccgatga cgatgcccag ttcctgcagg cgcgagcgga aggcgttacg gccggagtgc
     8581 ttgccgagca ccagcttgtt ggcggtccag cccacgtcct cggcgcgcat gatctcgtag
     8641 gtttcgcggt gcttgagcac gccgtcctgg tgaatgcccg actcgtgggc aaaagcgttg
     8701 gcgccaacga tggccttgtt gggctgtacc gggaagccgg tgatgccgga caccagcttg
     8761 gaagccggca cgatctgggt ggtgtcgatg cggctgtcgc acaggaagat gtccttgcgc
     8821 gtgcgcacgg ccatcaccac ttcttccagc gaggcattgc cggcgcgctc gccgagaccg
     8881 ttgacagtgc attccacctg gcgggccccc gcgcgcacgg cggcaaggga gttggccact
     8941 gccaggccca ggtcgttgtg gcaatggacc gaccacacca ccttgtcgga accgggaaca
     9001 cgctcgatca ggctgcggat cgtcgcggcg aactgctcgg gcacgttgta gccgacggtg
     9061 tcaggcacgt tgatggtggt cgcgccagcg cggatgacgg cttcgaagat gcggcacagg
     9121 aaatcaagct cggagcggcc ggcgtcctcg gcggagaact cgatgtcgtc ggtgtactcc
     9181 cgcgcccagc cgatggcctt cacggcctgc gccaccacct cgtcggggct catgcgcagc
     9241 ttcttttcca tgtggatcgg gctggtggca atgaaggtgt ggatgcgccc ggagcgggcc
     9301 ggacggatcg cttcgccggc gcggcggatg tcgttctcgt tggcgcgggc gagggagcag
     9361 acggtggaat ccttgatcgc ctcggccacc gccttcaccg cctcgaaatc gccattggac
     9421 gccgccgcga aacccgcctc gatgacgttg accttcatcc gttccagctg gcgggcgatg
     9481 cgcagcttct cctcacgggt catggacgcg ccgggactct gctcgccatc gcgcaaggtc
     9541 gtgtcgaaga tgatcaactg gtcgttcatg ctgggctcct gccgggattc ggactttcag
     9601 attagccgac gtctcggcca cggcggccga aaacgtttca atgaagggga tagtgtgttc
     9661 agcgcgcggg gcgcagtcgc agatcgcgaa ggacgggccc accgccacgg acaggcggtc
     9721 tcccggagca agcggaaatc ggaagacggg gaggatgcat cggcggacta taaatcagat
     9781 ttttccgccc cggcaaccgc tgccttgcgt cggccgagcc agcgccacgc ggcaaacaca
     9841 tagccggacg cggcgtagca cacgaacagg ccgaacagca caccctgggg atagctcgac
     9901 accagggcaa agcctgccac gaaggcgatc accacgaaga aaggcacgct gcggcgcagg
     9961 ttgatctcct tgccgctgta gaacagcacg ttgctgacca tggtgatgcc ggcgaagatc
    10021 gtcagtgtgc aggccaccca gcgcacgtcc tgaccgctca ggccgttatc gatgaccacc
    10081 cacaccagcc ccgccaccag cgccgctgcg gccgggctgg gcagaccctg gaagtagcgc
    10141 ttgtcgatga cgtcgatgtt ggtgttgaag cgggcaaggc gcagcgcagc gccggcacaa
    10201 tagatgaacg cagcgatcca gcccagcttg cccatgccct tgagggccca ctcatacacg
    10261 atgagggacg gcgcggcgcc aaaagacacc atgtctgcca gcgagtcgta ttcggcgccg
    10321 aaggcgctct gggtgcgtgt gaggcgcgcg acgcggccgt cgaggccgtc catcaccatg
    10381 gccacgaaga tcgccacggc cgcctgttcg aactggccgt tcatggcctg cacgatggca
    10441 tagaagccgg cgaacagggc cgcggtcgtg aacaggtttg gaaggatgta gatcccccga
    10501 cgacgtcttt cggggttcaa caggggcttg cgggcgtacg gatcggccat ggaggggacg
    10561 atgggtgagg tcgtggaatt gtacccgatg cggaccgaca ggcggaaagc ggaacaaccc
    10621 gcgcgcctca tggcggcccg tcaacgacaa aagggggact gtcgtccccc ttttgcaggc
    10681 tgcggatggc caggctcagt tcttggactg atccaccagc ttgttggcct tgatccacgg
    10741 catcatgtcg cgcagctggg cgcccaccgt ttcgatggga tgcacggcat tcagacggcg
    10801 acgggccgtc atggccgggt agttggtgcg gccttccaga atgaacatct tggcgtagtc
    10861 gccggtctgg atgcgcttca gggcgttgcg catggccgcg cgggactgct cgttgatgac
    10921 ttcggtgccg gtcacgtact caccgaactc cgcattgttg gagatggagt agttcatgtt
    10981 ggcgatgccg ccttcgtaca tcaggtcgac gatcagcttc agttcgtgca ggcactcgaa
    11041 gtaggccatt tccggcgcgt agccggcttc caccagcgtc tcgaagccca tcttgaccag
    11101 ctcgacggcg ccgccgcaca gcacggcctg ctcgccgaag aggtcggtct cggtctcttc
    11161 gcggaagttg gtctcgatga cgccaccctt ggtgccgccg ttggccgccg cgtaggacag
    11221 ggcgatgtca cgggccttgc cggacttgtc ctggtagacc gcgatcaggg acggcacgcc
    11281 gccacccttg aggtattcgg agcgcacggt gtggccggga cccttggggg cgaccatgat
    11341 cacgtccaga tcggcgcggg gcacgacctg gttgtaatgc acgttgaagc cgtgagcgaa
    11401 ggccagcacg ccgccctgct tgatgttggg ctcgacgtcg ttgcggtaca cctcggggat
    11461 gttctcatcc ggcagcagga tcatgaccac gtcggcagcg cggacggcct cggccacttc
    11521 tgccaccttc aggccggcgc cttcggcctt ggaccaggac gcgccgccct tgcgcaggcc
    11581 gaccgtcacg ttgacgccgg agtcgcgcag gttctgggcg tgggcgtgac cctgggagcc
    11641 gtaaccgacg atggtgacct gcttgccctt gatcagggag aggtctgcat ccttatcgta
    11701 ataaaccttc atttcatgcc tttctgacac aggataagcg caggcgcgga acgcgccgca
    11761 tcacactttc aagacccggt cgccgcgacc cacgccgcag atgccggaac ggacggtttc
    11821 gagaatcagg gccggatcga tggcgccgac gaaggaatcg agcttggcct gggtgccggt
    11881 gagttcgatg acgtaggtgc tctcggtaac gtcaatgatg cggccgcgga agatgtccga
    11941 cagacgcttc atctcgtccc ggtccttgcc ggtggcgcgc accttgacga gcatcagttc
    12001 gcgctcgatg tgggccgatt cggacagatc gaccaccttt accacctcga tcagcttgtt
    12061 gagctgcttg gtgatctgct cgacgatttc gtcggagccg ctggtgacga tggtcatgcg
    12121 cgataccgac gcatcctcgg tcggtgccac ggtgagggat tcgatgttgt agccgcgggc
    12181 cgaaaacagc cccgagacgc gcgacagggc gcccgattcg ttttcgatga gaagggaaat
    12241 gacgtgtctc atgtgcgtgg tcgaagaaat gggcagatag cggtgcaacc aggcgccgct
    12301 ggcggaccgg ggcgaaaccg gcatcgccgt gtcgcgcccc gccgtcaccg gcttacaggt
    12361 cctcggcgga caggatcatg tccgtgagcc ccttgccgcc ctggaccatg gggtagacgt
    12421 tctcggtcgg gtccacctgg aagtccagga acaccagatc gttcttgcgc tggaaggcct
    12481 cccggagggc gccttccaca tcggccggct tgtccacgcg gatgcccaca tggccgtacg
    12541 cttcggccag cttgacgaag tccggcaggg aatccatgta ggactccgaa taacggttgc
    12601 cgtggaagat ctcctgccac tgacgcacca tgcccaggta gcggttgttg agcatcacca
    12661 ccttgatggg cagacggaac tgcttgcacg tggcgagttc ctggatgttc atctggatcg
    12721 aggcttctcc ggtgatgcat gccaccggcg agccgggatg ggccagttgc acccccatgg
    12781 cggccggcat gccgaagccc atggtgccca gaccgccgga attgatccag cgccgcggct
    12841 tgtcgaactt gtagtactgg gcgccccaca tctggtgctg gcccacgtcg gaggtcacga
    12901 aagcgtcgcc accagtggct tcccacagct tctgcacgac gaactggggc ttgatgatgt
    12961 cgtcgggctg ggtgaacttg aggcaatcgc ggcggcgcca ttcctcgatc tgcttccacc
    13021 acacgtccag cgcttcagca tccgggcgct cgctcgtccc ctcgagcagg gcgagcagtt
    13081 cacccagcac ttcgcccacg tcgccgacga tgggcacgtc aaccttcacg cgcttggaga
    13141 tggacgacgg atcgatgtcg atgtggatga tcttgcgcgg ttcggaggcg aaatgatccg
    13201 gattgccgat cacccggtca tcgaagcggg cccccacggc cagcagcacg tcgcagtagt
    13261 gcatgcccat gttggcttcg taggtgccgt gcatgcccag catgcccaga cactggcggt
    13321 cggtggcggg atagccgccc agacccatca gcgtgttggt gcaggggaaa ccgagcctgc
    13381 gaacgaggtt cgtcagcttc tcggaagcat cgccgagcac cacgccaccg cccgcgtaga
    13441 ccatcgggcg cttggcttcc agcaggagtt gaagcgcctt gcggatctga ccgtgatggc
    13501 ccttgaccac cgggttgtag gaccgcatcg acacgctcgc cggatagacg aactcgcact
    13561 tctgggcggt gatgtccttg gggatatcca ccagcaccgg cccgggacgg ccggtacgag
    13621 ccagatagaa ggctttcttg agcgtgggcg cgatctcgcg cacgtcccga accaggaaat
    13681 tgtgcttcac acagggccgg gtgatgccga ccgtatcgac ttcctggaag gcatcctggc
    13741 cgatcgccgc ggaaggcacc tgcccggaga tgaccaccat cggaatggag tcgcaatagg
    13801 cggtcgcaat gccggtcacc gcattggtcg caccgggccc ggaggtcacc agcgccaccc
    13861 ccacccgatc agtggaacgg gcatagccgt cggcggcatg gatggcggcc tgttcatggc
    13921 ggaccagcac gtgacgaacc ttgtcctgct tgaagatttc gtcgtagatg tagagcacgg
    13981 cgccaccggg atagccgaac agatagtcga ccttttcttc ctggagacac ctgattacaa
    14041 tttccgcacc ggtcaatacc attgctgcac ccgaagctgt ctcagccatg tgaaacgttg
    14101 aaccataccg ccgacccggc gtttggtcaa ggtgacgttt ctgtggtggt ccgatacagt
    14161 ggcgggtgct ttgtcgtttg agcggctaca gctcggcttc gcccgcgagg aactcgcccc
    14221 tgtcttcccg aatcgaactc tccgactttc tcgccagcgt cgagaagcgc gccttcaagc
    14281 aggcgctctt tgcggtgcgc aacgaggaga ctgccctcga catcgtccag gacgcgatga
    14341 tgaaactcgc ggagaagtac ggcgaccgtc cacccgaaga gttgccgatg ctcttccagc
    14401 gcatcctgca gaacgtcatc cgcgaccact accggcggca gaaggtccgc agcatgtgga
    14461 ccaccctgct gtccgcgttc agcggcccgg acgaggagga tgagcacgac ccgctggaga
    14521 ccctggaatc cgccgacagc gagttccgca gcccgtcccc ccaggcggag gttgaacagc
    14581 aacagaccct gtccctgatc gaacgggaaa tcgaaaagct tccgccgcgt caacgcgaag
    14641 cgtttctgat gcgttactgg gaagaaatgg acatcgcgga aacggccgcc gccatgggct
    14701 gttcggaggg cagtgtcaag acccactgct cccgggctac tcatgcattg gccgcagccc
    14761 ttgcagcaaa aggcattacc ttatgatcga gcacgaaatc caccggcgtc tgcgtcaaca
    14821 tctcaaccgc tcgctgcagc gcatcgaacc ggacacgtca gagcgcctgc acgccgcccg
    14881 acgcaaggcc ctgtcacggc aaaaggtggc tgtccacggc ctcagtctgg ctggcgcggg
    14941 gcgtctgatc accgaccacc tgccagaccg gcggactgcc gtcgccatgg tgctggttgt
    15001 cgtcattgcc ctggccagcg gcttcctcgg cgatctgcaa cgcacggccg acatcgaaga
    15061 ggtggacagc tcgctgctcg ccgatgacct ccccatcgac gcctacctgg accgcggttt
    15121 cgacacatgg gtgcaaagcc aagctctcga gtaattgctg cgctggccct gtgctgggcc
    15181 gcccacgctg ccgccgtccc gccgctcaag gccccttcct ggaacgaact cacgccggcc
    15241 cagcagcgca cccttgcgcc gctcgtcaag gaatggagca acctcccgga cgaccgcaag
    15301 cgcaagtggg tcggcatcgc tgaccgctat cccggcctca cacccgaaga gcaggccagg
    15361 cttcagcagc gcatgcatga atgggcctcc ctcacccctg cccagcggga gtctgcgcgg
    15421 gagcagtacc gcaacctgca aaagatgccg cccgaacagc gtcaggcctt gcgggaaaag
    15481 tgggaagcct atcttgagct gccggaggag gagaagaagc gtttcaccga cgccgcgccc
    15541 aagcccggcc ccaaaccgcc tgtacccgga caaccggcca caccgatcaa gcccctgact
    15601 gcaccgccgc agccggcgcg cccggtcgtt ctacccaagc cggtaccgcc gcccgcgccc
    15661 ctcacggccc ccgctccagt gcctgttcct gtgcccgccc aggcgcccgt cgtcgccggc
    15721 caggatccca ccccacccaa accctgatcc atgatgcctt ccaccgccac gtccggagcg
    15781 acgccccggc cgacgggtga gctcgccacc ctgcgccgac gcctcgccag catgctctac
    15841 gaagtcctgc tcctgctcgg cgtacttgcc ctcaccttcc tcgtgccgct gctgatcctt
    15901 gggattggct tcggcatcct gccacccggc ggtgtcatgt ggttgtggat ctatctggcg
    15961 ctgggcggat atttcgtgtg gtactggcgt cagggcggcc agacgctggc catgcagaca
    16021 tggaagctgc gcatcgttgc ggcgaacggc gacaccgtat cggtccgcca ggcctggctg
    16081 cggtactcgc tggcctgggc ctcgctgtgc gtcggcggcc tcggcctgtt ctgggccctg
    16141 atcgacccgg agcgtcagtt cctgcacgac cggctggccg gcacgcgcat cgtcctgctg
    16201 cttgccccac cggctgactg aagacgcctc agcgccgctc gacccaccac atcatcgtcg
    16261 ccgccgcgac aaggaaggtg acgctagggg tcagcgccgc gaccgccggc acccagccgt
    16321 tgatcacacc gaggttggag aacagaccgt tgagcaggtg gaagccgacg ccgatcatga
    16381 tccccgagaa gaccttcagg ctcaccccac ccatgcggtt ctgcatgtag gcgaagggca
    16441 gcgcgaggcc catcatcacg agggccgcaa agggatagac gagcttcttc cacagggcga
    16501 tctcgtagcg ggtggtgcgc tgattgttgt cttccagatg gcggatgtag gaataaagcg
    16561 tcgtcacgga catccgctcc ggcaccacca tcagcaccga cagcacgtcc ggcgtgagtt
    16621 cggaatgcca caccagctcg ggcagggaga ccgtttctgt gcggtcgccg aggaagcggg
    16681 tttgcgccac ctcgctcagg cgccagccat tgcgctcgtc gaacacgccc cgcgtggcct
    16741 cgctgatgga caccagggca tggcggtcat cgaactcata gagccggaca ccttccagcg
    16801 tggtgtcggg catcacgttg cggacattga cgaaacgatg accgtcccgc acccacaggc
    16861 cggtgcgcag ctcctgggac accatggagc gggtcgcggc cagacgccat tgctgggcgg
    16921 cccgttcggc gggcggcgcc aggtattcgc caaatacgaa ggtgatcgcc acgaagacgc
    16981 tcccgatggt caggagcgcc gccaggaagc gcccggtcga caggccggat gcccgcaaca
    17041 ccgtgatctc ggaatgccgc gcgagggtgg tgagcgcata cagcgtaccg atcagcaccg
    17101 cgatcggcat cagctcgtag acccggcccg gcaggatcat cgccacgtac agggccgcgt
    17161 ggtagagcat gtaaccgttc ttgcccacgt cgtcgagttc attgaccaga tcgaagaagg
    17221 cgaacaaccc gaggaaggcc agcagcacca tggccacggc ggcgtagatt tccctggcca
    17281 gataacggac gaagatcggg gccatcagcg ccgcctccat cgcgcgaaca cgctcaggcg
    17341 cttccagaac agcaccgcca gcaacagcat catcaccaca tggggtgcca tcagcgccac
    17401 ctcgaagccc agccgaccct gcgccaccca ggcctgactc acgctgaggg cgttgctata
    17461 gatcagatac accaggatcg ccaggatcag gttgttggtc cgtcccgcgc ggggattgac
    17521 aaaggacagg ggaatcgcca gcagcgccag catcacggcc agcacgggcg tactcagccg
    17581 ggccagcagt tccccccggt acaggttgcc cttttccttg agcaaggccg ggaaggtcag
    17641 ggtcttgggc agacgcgggc ccgtcaccgc ctccttggac tgcatgcgga tctggtagcg
    17701 ctcgaactcc atcacccggt agctcggggt acccggcacg ccttcgtagc gccgaccgtg
    17761 ttcgaggatg acgaagcggt tgcccaggcc atccacctcg gaatgaccgt tggccgcgac
    17821 catcacgccg agtcggccgt tctgcatgga actggcgaag acgttgcgca cgcgcccttc
    17881 ctcacccgag gcggcctcca cgaagaacac ccgctcgccg ccggaggatt cgcggaacat
    17941 gcccggcgcc acccgcgaca cgtcgtcccg gttgttgagt tgctccttgt attcaacgct
    18001 tttcttctgg gcccagggcg ccccgaagag agacagcagg gccaccacaa tcaccaccgg
    18061 cagcgcgaag cgcaacaccg gccggaccca tgcggtgagc ggcaggccgc aggagaacca
    18121 caccaccatc tcggaatccc ggtaggcccg cgagagggac atcaggatcg cgatgaagac
    18181 cgtgagggac agcagcaccg gcatctgggc gatggcgccg aaaccgatca gggccagcac
    18241 cgcatcggcc ggcacgcggc cacccgcggc ctggcccagc aggcggatca gcacggtgga
    18301 gatgaggatc gcgaacagcg ccacgaagac cgccacggcg ttctgggtga attcgcgctg
    18361 ggcggctcgg tgaaagatca tgcaggcggc ggaaggggtg cgggtacgga acggcggggg
    18421 acggcgggcg aaccgctttg acgtcggccg gggtgctgcc cataatggcg cgatgtccga
    18481 ttgacgcgtg tttccaggga gaagcgggtg gaatttacca taaaggccgg gtcgccggaa
    18541 aaactcaaaa gcggctgcgt ggtggccggc gtgtttgccg gcggcgaact gagcgcagcc
    18601 gccaagaccc tcgacaaggc ctccagcggc gccctcgcga ccatcctcgc gcggggcgat
    18661 ctggacgaca aggccggcgc caccctgatg ctccacggcg tgccgggcat tgaagcgccc
    18721 cgcgtgctgc tggtgagcct gggcgctgcc agcgaactga ccgacaagcg ctaccgtgac
    18781 gctgtcgccg ccgccgcccg gcagctcgcc gcctccatgg ccggcgacgc ggccttctgc
    18841 ctcgccgagg cggacgtgcc cggacgcgac ctggcctggc gcatcgccca ggctgcgcag
    18901 atcctccagg acagcaccta ccgctgcgac gagctgaagt cgcgcaagga ggactcgaag
    18961 aagggcgttg ttcacgtggc cttcatcacc cgtgccaagc acggcgacga actccaggcc
    19021 gccctccgcc gcggcgaagc catcgccgaa ggcctcgccc tcgccaagac cctcggcaac
    19081 ctgccgggca acgtgtgcac gccgggctac ctggccacca ccgccaagaa gctcgccaag
    19141 gaacacaaga tcaaggtcga gatcctcgac cgggacgcca tggaaaagct cggcatgggc
    19201 gcgctcctct ccgtggcccg cggctcccat gaggcgcccc gcttcatcat cctccagtac
    19261 aagggcggca aggccaagga caagcccgtc gtgctggtgg gcaagggcat caccttcgac
    19321 accggcggca tctccctcaa gccgggcgaa ggcatggacg agatgaagta cgacatgtgc
    19381 ggcgccgcca gcgtgctggg caccttcaag gccatcgccc gcatggagct gcccctcaac
    19441 gtgatcggtc tgatcccatc caccgagaac atgcccggcg gctccgccac caagcccggt
    19501 gacatcgtca cctccatgtc cggccagacc atcgaggtgc tcaacaccga cgccgagggc
    19561 cgcctgatcc tctgcga
//
LOCUS       DAOQPA010000065        19379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1410_length_19379_cov_18.849100, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000065 DAOQPA010000000
VERSION     DAOQPA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19379
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1410_length_19379_cov_18.849100"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKX01_10310"
     CDS             <1..497
                     /locus_tag="PKX01_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMZ76563.1"
                     /translation="AQFNTEKLKWLNAHYIKQSDTARLAADVARRLARRGVDPEHGPA
                     LEAVVALYKDRVSTLVELADAVEPYYIDLHPAPELLAQHLTDAARAALATLRGKLADV
                     AWEKALLNAAIKETCAAHGIKMPQVAIPLRVVLLGQPQTPSIDAVLETMGQALVLGRL
                     DRVL"
     gene            complement(514..1872)
                     /locus_tag="PKX01_10315"
     CDS             complement(514..1872)
                     /locus_tag="PKX01_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76564.1"
                     /translation="MGFLPLIEAFLALGLTMIVLTTGVSSLAGTWQRLRRARARGLQS
                     LLRCTFLLAVKNIEKPASAGSEDPWQTFCAQRDAPDALIGFVAEMCLQPTALADCAAL
                     TGDARRAAYRRLLPDLAPAEAPSNGDSGRDASVPSRMAHLADRLLHAFRRWRARAFTL
                     DTLSDEEFRTRFTHSTLGAALCAADEAHFPARLDALNGQFIAYGHAASEAFAKDARLH
                     SLCCAAGLVMLANIDAFNLLENYLAQPVLRAAIISRYEHAASGSSLPAGGATATTAVP
                     AVASQFDAVLRNAADAARKAKDLPLANAIEQKALEMRTSIDTLQTARNEALQTVADAT
                     AGLPVGWDRYPFCAAGASDTRCRGLTPPSPATDADARLRTRIEAFVGRVGEVADVDAG
                     NFLRWLAGLILTMVMLGLGTPFWVQAVNGLLRARDLVRGGNKAGDGPDGPPPAAAPPA
                     NR"
     gene            complement(2058..2807)
                     /locus_tag="PKX01_10320"
     CDS             complement(2058..2807)
                     /locus_tag="PKX01_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HMZ76565.1"
                     /translation="MDTSSPRPGLHPMLWVAAASVTALSLAGVARLTGLLPASSPEPA
                     PVVAVQPAPADTPVAPPAAPKVADAPVAEPPARVDHAPVPRHETPKPKAAAKTTPAPA
                     ATAQGAEPVVPAGRPVADVPPPPPVCRDCGVIESIETHQVKGEGSGLGAVGGALLGGI
                     LGNQVGEGSGKKLARIGGAVLGGFAGNEVERRERSTVRYDVTVRLDDGTRRVLTQSQA
                     PAWREGDRVRLHNGEILPYAPPASGAPRSSF"
     gene            complement(2959..4872)
                     /gene="mnmC"
                     /locus_tag="PKX01_10325"
     CDS             complement(2959..4872)
                     /gene="mnmC"
                     /locus_tag="PKX01_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HMZ76566.1"
                     /translation="MSLIPARFTPGPANTPYSLQFDDVYHTASGGLGQARHVFIAGNE
                     LAARWKGRRTFTVLETGFGLGLNFLATWHAWAADADRCQHLHFASVEKHPLTRHDLAR
                     VLSDWPELAPQAAHLLNAWPNLVPGIHRFTFEQGRVVLSLALGDALQVLPELTMRADA
                     FYLDGFSPDRNPDMWSPAVFTQLARLAAPGATLSTWCVAGHVRRGVQEAGFIVERQPG
                     FGGKRHMLAGRFPGPGPTSTGIPDSHRALVIGAGVAGTSVAERLAARDWQITLIDSAD
                     APGQGASGNRAGVFRPQPSLDDNRLARLTRAGFLYGRQHLTQLAERGHPVRWAPTGVL
                     HLARDAKQENKMRAVVEAQQPPDAYLRYVTQEEAARLADWPVSAGGWWFPGGGWIQPP
                     SLCAASLAAGGTRIIPLFGRTLARLERRDGEWAALDADGHEIARAPIAILANGVGLRA
                     VPEAAMLPVRPARGQVSHLPAASGSAPGIVVCRLGYVSPEVDGVRCAGATFVADDLDP
                     QLRDSEHRDNLAKLDFILPGYAADYDPATLDGRVGFRPASPDRLPMVGAIPAVAPVDR
                     TTPLADIPRQPGLFAVSGFGARGLVWASLMGELLASQLNGDPLPLERDLVDAIDPARY
                     LLKPPRPMRGEDG"
     gene            5165..6073
                     /locus_tag="PKX01_10330"
     CDS             5165..6073
                     /locus_tag="PKX01_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ76567.1"
                     /translation="MAILMGCYFGQDYLAAQLDSIQAQSYRLWKVWAADDGSTDATPE
                     LLERYQASWGRDRLAVRRGPQQGFVANFLALVCDDGIQADYFAYSDQDDVWAPEKLAN
                     AVAWLASVPAGIPALYCSRTRLFKGARTVGCSPRHRRPPSFANALVQNIAGGNTMVFN
                     RAARRLLAHAGPDVKVVAHDWWTYIVVTACGGQVHFDEQMTVDYRQHEDNQIGAGNGW
                     AFLVRRIEGFFNARFRCWVDGNLVALERLGACLSPESKRIFALFSAARKGGLSARLSG
                     LLRSGVHRQTAGGNCGLLIAALFNRI"
     gene            6217..6969
                     /locus_tag="PKX01_10335"
     CDS             6217..6969
                     /locus_tag="PKX01_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ76568.1"
                     /translation="MALARREVVGRYRGSFMGILWSFIHPVFMLAVYTFVFSVIFRAR
                     WGGGSEGREEFALVLFAGLLVFNVFSECVTQAPGLILSNVNYVKKVVFPLEILPGVTL
                     VSALFHCGISFVVWLVADVLLFGVPPVTVLLFPLVLLPLCLFVMGLSWLLASLGVYVR
                     DLAQFIGLATSVLMFLTPIFYPATALPEAYRAFLFLNPLTPVIEQARDVLCWGKGLDG
                     AEYALTLCASALFAWAGFAWFQKTRKGFADVL"
     gene            6959..8344
                     /locus_tag="PKX01_10340"
     CDS             6959..8344
                     /locus_tag="PKX01_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76569.1"
                     /translation="MSSEVVIRVAGLSKCYHIYDKPHHRLMQMLSRGRRTYFREFWAL
                     RNVSFEVRKGEAVGIIGRNGSGKSTLLQLICGTLNPTSGSVETRGRIAALLELGSGFN
                     PEFTGRENVFMNGAVLGLTREEMEARIADIEAFADIGEFIDQPSKTYSSGMLVRLAFA
                     VQVCIEPEILIVDEALAVGDALFQKRCFERMEHLRSNGTTLLFVSHDQESVRALTNRA
                     VFLHHGEVQHTGNSADVLLEYRRLLHEEETRALGEAAARLRNRAAEQRAQQAARPVAP
                     ATAASPAAVDAPAPSRSAELSFGDMDAEVVSVSVFNAGGEPCSHFDPRDVAQVVIQCV
                     ARRDLDRLNVGLRIRNKEGVKIYSWGTLNQDMAIRAGLADGEVFWNRSFKAGEVFSVS
                     LDFVVGLGPNLYEIQASITQEGKPYYAEQRMLHWMDEAAFFTVATDPRTYHFGGVVDM
                     RMSSRLLGGAS"
     gene            8341..>11366
                     /locus_tag="PKX01_10345"
     CDS             8341..>11366
                     /locus_tag="PKX01_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ76570.1"
                     /translation="MSGTPAGGMTPALADAVIEEVLLACGGGTLAVVGQGAAHCVRAA
                     LRIGIDARGVEVSDAGAVEWARTAMPGRVVAGSVCALPYADGAVDTLVVLHGLETLDE
                     DDVPTAIAEIGRVASRAVYLRMATSPASGADGVFRSRGGWEQPLFSAGFRKHPSYYRV
                     NAYEALEGEAGEITIVLEKLPAPALKRYPLAALAEERDLHMDMLRETGARSDAHVARY
                     QWAAAFVRPGDTVLDAACGLGYGSYFLQTGTEAACTLGIDGSAYAIDYARENFSAIVP
                     GLEFREGFLPGALASIPDHSVDVVVSFETLEHVDDNLALLAEFHRVLTPAGRIVVSVP
                     NDWSDETGEDPNPFHVHVYTLERLRRELGAHFILEKLVAQSASQHKSGPDRTAWTAAG
                     RSLRDVSPDVADDQAPDAEWWLAVAMRSPLEGQDVPYRETQFPQFPSPDWNVTAFGRD
                     YRNPWLVRGMVDIAHRLQAPGALHVLAEEVAATAALDTADAGAALCVLAYQMLDAPET
                     TCAAVEALDRRVMVYLDEDPPSPHGQRWCVSLQFVLGRLWMGVGDFARAAESLEGCIA
                     RGSDRFSPLLDNRVVEACLILGQLNLHQGHRDSALRWWSTGIERARRAVGGDWSGAIG
                     DLAHPAEFGLPELASILEYASACAYALVHVDDVDVKPWWWLHPRRDRLSRHAAVLREL
                     VCQREQLAVRDRELAAYRAQSDAYREQLSRAVDAARDTAAYSERLAGELGSYQGQSDA
                     YRQQLDAAQTTTRELSGYAERLVRELAALKAESEEYARSVRKAQADVIDATASRAEVE
                     RALSESRNVAATLERRQAEAEARLSAADRLVAGLQGEVAAYARESASYAEQIQAAEAR
                     LQESAAYASTLLSQIEAYQRQSVEYCEQIRGAETQLQAASEHIASLQDQVVAYERQAS
                     QHAEQSRAAEARLQEGAAYASTLLSQIEAYQRQSVEYCEQIRGAETQLQAASEHTARL
                     LEHVAACAGESQAYRTQLDEAVSRLSEFDAYVRRLEA"
     assembly_gap    11367..11466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11467..11966
                     /locus_tag="PKX01_10350"
     CDS             <11467..11966
                     /locus_tag="PKX01_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76571.1"
                     /translation="EAVSRLSEFDAYVRRLEAELTAYQTQADVHEKRLAAVQQDADSA
                     AASVRNLERDAVRMRDQLDREARRLGVSEATRTALERSLADAEGRVATFIEQEMVHRS
                     RIGALESQVECLEQERNAHQVQYRSMLTLYSSRMASPRFLLRRLWHLILVRVGLIRRR
                     ESEEQ"
     gene            11963..13711
                     /locus_tag="PKX01_10355"
     CDS             11963..13711
                     /locus_tag="PKX01_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ76572.1"
                     /translation="MKIVLTVHQFPPEYISGTEVLTFSVAKDLMRRGHEVRVLTGAPA
                     KAPLRDAERFDEYEIEGIRVYRFTHEFVPMGDQNVIMEMEYTNRLVARYFQSLLDEFQ
                     PDLVHIFHMSRLGTSIIDVATGMKIPTYFTPTDFWSACPTSLLMLDDGTPCKGPEGAG
                     GNCVRHVAMRARWSRIAKAAKYAPDFLADQVVTLARKGWLPRQMPFRNEISALSRRHE
                     FVISRLNALNGIVSPTNLMTDALTRNGVRPELIRQHMYGINIDNYNDVIRAHVQGRTL
                     TFGFIGGLSSHKGCHVLIKAIRKLTRADVRLKIYGNPKDSPAYFAELQSLADGAPFIE
                     FCGTFPNGKIAEVLSGIDALVVPSVWFENAPLVIHSALAARCPVVASNFPGMSEVVRH
                     GENGLLFEPGNPDSLADTLRRVLEEPTLLSELSSRCRPPKSIAEYVDEILAVYASPPA
                     PEGGRGIVEFRRFEPMDPRNEASFVSGWCIAKAGEPMSVEIRVNDRVVAQTRQFAPRP
                     DVRAGFGGAGLVIRGEQFGFALSFPAGIQRINASVRIAAPDGQTRDLSMSELAAGSMV
                     KVGDDFFIGLDQECFS"
     gene            13792..15648
                     /locus_tag="PKX01_10360"
     CDS             13792..15648
                     /locus_tag="PKX01_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76573.1"
                     /translation="MDGKPWLQALFFVLCAGVYLFLMNAHKAAELADDGAFFLRYAVN
                     MLHGHFWVWNIGEAPVWGASAPLFPVFLTLPLMFGFDPVTTVMNAGIVAGAAGLSVIA
                     WLLAKRISLVAGLGFLAFSATSSQITYWVGSGLETPLTIILLAFALFSIFGTDRPWIA
                     GVAAGLLMIHKLDMVPVGLLVLAAAWLRDRRVPATAAVIALLLSGAWYLFAWWYFGAP
                     VPNSFITKALHQSHLVPIIDWRWYGQFVLLSGINKYASVCLLGLAWAIRPSRPVLCLF
                     GGLLLIHLTAYTIKHPVEPYAWYGMGAMTSLFVLASVGLDGVARQLARLRSGHQGALL
                     GDAVVLVFLVTAVLAVQRHERGLSEGFKVWADNYERDRADAGRWVAAHTPESYVVMAG
                     HGNPAYFSGRKVIDSSYLNRRYEAGNVLERLRPEIYISPGNPSDPDFSKPLFSDYQVV
                     KIFDRTFSSGVDSKYWFAVLARKDAVGRMSGLNAGPTAPVSVEKYVSDVSLGDQFGVL
                     RSEGSDTLFIHPGATTPTGFDFDASGFAAGYGGRALQVTVSVSPKVPPEAVARGAAVA
                     RVTFSRKGEVAAQSIVKVGSPFHFTMPANSGWRVVVDNYNGPDTDWILISFN"
     gene            15664..16155
                     /locus_tag="PKX01_10365"
     CDS             15664..16155
                     /locus_tag="PKX01_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76574.1"
                     /translation="MRLKNLKSLVVAGCFVAGLVACESGPNVPAGEESKFLFALKTSE
                     TIGDQVGNVALNENGIMIHPGATAPTSVSFNLDGKLRAIGIKPYIAPLNEEGKAHADA
                     GIVGVELLVDGKSVDKFTVDRNFSQEKTLDVKGAKSLEIRVDNGNGTPIWDWFHVKVT
                     SVK"
     gene            16202..17146
                     /locus_tag="PKX01_10370"
                     /pseudo
     CDS             16202..17146
                     /locus_tag="PKX01_10370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            complement(17270..17728)
                     /locus_tag="PKX01_10375"
     CDS             complement(17270..17728)
                     /locus_tag="PKX01_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HMZ76575.1"
                     /translation="MRNFDEIDRKILGILQRDGRITMTDLAPQVGLSVTPCTERVRRM
                     ERDGVIAGYHARLNPRALGLNALLFLELRLTSRSAEALTQMSETLAEIPDIMECHYVS
                     GDFDCLVKARLPNLGEYRPFIADLVERLPVVESRSYVVMEEIKESPLLNA"
     gene            complement(17843..18379)
                     /locus_tag="PKX01_10380"
     CDS             complement(17843..18379)
                     /locus_tag="PKX01_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HMZ76576.1"
                     /translation="MSSLFTNRFKTLLAVAGIAVATPFAAHAFGGHEHCGGPMMHREM
                     GFGGPEGGAMHLPPFVRELKLSDAQRDQIFKIMHEQAPAFRDKAIAARKAHEELRDMA
                     FSARYDEARVAALSDTEARAMAELTQMRLRTGNQIYQVLTPEQKKKADELKADFETRM
                     TRPVRDGGQPGKREPLRN"
     gene            complement(18477..>19379)
                     /locus_tag="PKX01_10385"
     CDS             complement(18477..>19379)
                     /locus_tag="PKX01_10385"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ76577.1"
                     /translation="ADRIGFQLLTGAGFDPRAMASFFDRLQKATRLVDGNVPSYLRTH
                     PLTHERIAEAEDRAFNLSYKQVRDSDEYHFVRALLRSYEGEPREAVALLDKALAEGRT
                     QNARATRYGLTAALLRAGDFKRADKELAVVERGGPHPMVEALAGQILLQSGRLPEARK
                     RYESALQRFPDHLQLVYDYPRVLIRLNDEAAAARFTESRLLARPQDPTLHRLAAEAFA
                     ALGQQTRSHVHQGEFEAARGNTQAAIEQYRIALRSADGSERDLTIADARLRELQAQQR
                     ELRKDGRDGGKDPSGGRLHSFTPR"
BASE COUNT         3022 a         5788 c         6906 g         3563 t
ORIGIN      
        1 ccgcccagtt caacaccgaa aagctcaagt ggctcaacgc ccactacatc aagcaaagcg
       61 acaccgcccg actcgctgcc gacgtggccc gccgcctggc ccggcgtggc gtggatccgg
      121 agcacggccc cgcgctggag gcggtggtgg cgctgtacaa ggaccgggtg tccacgctgg
      181 tggaactggc ggacgcggtc gagccctatt acatcgacct gcatccggcg ccggagctcc
      241 tcgcccagca cctcaccgac gccgcccgcg cggccctcgc gaccctgcgc ggcaagctcg
      301 ctgacgtggc gtgggagaag gccctgctca acgccgccat caaggaaacc tgcgccgccc
      361 acggcatcaa gatgccccag gtggcgatcc cgctgcgcgt cgtcctcctc ggccagcccc
      421 agaccccgtc catcgacgcg gtgctggaga ccatggggca ggcgctcgtg ctcgggcgcc
      481 tggaccgggt gctgtaaggg cgcgacgcgc cgatcagcgg ttggcgggcg gtgcggcggc
      541 gggcgggggg ccatcgggcc catcgccggc cttgttgccg ccgcggacca gatcccgtgc
      601 ccgcagcagg ccgttgacgg cctggaccca gaagggtgtg ccgagaccga gcatcaccat
      661 ggtcaggatc agtccggcga gccagcgcag gaagttgccg gcatccacgt ccgcgacctc
      721 accgacgcgg cccacaaagg cctcgatgcg cgtgcgcaag cgtgcgtcgg cgtccgtcgc
      781 tggcgacggc ggcgtcagcc ctctgcagcg ggtgtccgag gcgcctgccg cgcagaaggg
      841 atagcggtcc cagcccacgg gcaatccggc cgtcgcgtcg gcaacggtct gcagggcttc
      901 gttgcgggcg gtctgcagcg tgtcgatcga ggtccgcatt tcaagcgcct tttgctcgat
      961 ggcattggcg agggggaggt ccttggcctt ccgggcagcg tcggcggcgt tgcgcaggac
     1021 cgcgtcgaac tgggacgcga cggcaggcac cgcggtggtg gcggtcgccc cgccggcggg
     1081 gagggacgat ccgctggccg catgctcgta gcggctgatg atggcggccc gcaggacggg
     1141 ctgggcgagg tagttttcca gcaggttgaa cgcgtcgatg ttggccagca tcaccaggcc
     1201 ggcggcgcag cacaggctgt gcagccgggc atccttggcg aaggcttcgc ttgcggcgtg
     1261 tccgtaggcg ataaactggc cattcagcgc gtcgagccgg gcgggaaaat gcgcctcgtc
     1321 tgcggcgcag agggcggcgc cgagggtgga gtgggtaaag cgcgtgcgga actcctcgtc
     1381 gctgagggta tcgagggtga atgcccgcgc acgccatcgg cgaaaggcgt gaagcagccg
     1441 gtcggcaagg tgtgccatcc ggctcggcac cgacgcgtcg cgcccggagt ccccgttcga
     1501 cggggcttcg gcgggggcga ggtcgggcag cagtcgtctg taggccgcgc gcctcgcgtc
     1561 gcctgtcagg gccgcgcaat cggccagagc ggtgggttgc aggcacatct cggcgacgaa
     1621 gccgatcagg gcgtccggtg catcacgctg cgcgcagaat gtctgccacg ggtcctcgga
     1681 tccggcggat gcgggtttct cgatgttctt caccgcgagc aggaaggtgc agcgcagcag
     1741 cgactgcagg ccgcgcgcgc gggcgcggcg caggcgctgc caggtgccgg ccagggagga
     1801 cacgccggtg gtgaggacga tcatggtcag gccgagggcc agaaatgctt cgatcagggg
     1861 caggaagccc atcgcatgta ctccgtcaac aggtcacagc ttcgtcgcag cgcaacgtca
     1921 tgtgcatgct gtccacagta gctgaagcat ctggcggcgc aagcgccgtg gcgagcgccc
     1981 gcccttaaac acgacggccc caccgatcgg gagatcgacg gggccgcgga caggggaggc
     2041 ccggacgggc cggtctgtca gaagctgctg cggggcgcgc cgctggcggg cggggcgtag
     2101 gggaggatct cgccgttgtg gaggcgcacc cggtcgccct cacgccaggc gggggcctgg
     2161 ctctgggtca ggacgcggcg ggtgccgtcg tccaggcgga cggtgacgtc gtagcgcacg
     2221 gtgctgcgtt cgcggcgctc gacctcgttg ccggcgaatc cccccagcac ggcgccaccg
     2281 atgcgggcca gcttcttgcc ggagccttcg ccgacctggt tgcccaggat gccgccgagg
     2341 agggcgccgc cgacggcacc caggccggac ccttcaccct tgacctggtg ggtctcgatg
     2401 ctctcgatca cgccgcagtc gcgacagacc ggcggcggcg gcgggacatc ggccacgggg
     2461 cggccggcgg ggacgaccgg ctccgcaccc tgggccgtgg ctgccggcgc cggcgtggtc
     2521 ttggcggccg ccttgggctt cggcgtttcg tgacggggta ccggtgcgtg gtccacacgg
     2581 gccggcggtt cggccacggg tgcatcggcg accttgggcg ctgccggggg cgcgacgggt
     2641 gtatcggccg gggcgggctg gacggccacg accggcgcgg gttcgggcga cgaggcgggc
     2701 agcagtcctg tgagtctggc aacgccggcc agactgaggg cggtcaccga ggcggcggcg
     2761 acccacagca tcggatgcag tccggggcgg ggggaagagg tgtccatggt gatgtgctcc
     2821 tgcggaatgt gttgatcagg ataacgcgcg gcaggtcgct caggtgacgt ttgaaatcgg
     2881 cgcttacaat gctgcagcgt acggatattc gtgccgcgcg ggcacatggg tgcgcagacg
     2941 gagggccggc gcggacgttc agccgtcctc gccccgcatg gggcgcggcg gcttgagcag
     3001 gtagcgggcg ggatcgatgg cgtccacgag atcccgctcc agaggcaggg gatcgccgtt
     3061 caactggctg gcgaggagtt cgcccatcag gctcgcccac accaggccgc gggcgccaaa
     3121 gccgctcacc gcgaagaggc ccggctggcg gggaatgtcg gcgaggggcg tggtcctgtc
     3181 gacgggcgcc acggccggga tggcgcccac catgggcagg cggtccggcg aggcggggcg
     3241 gaagccgacg cgaccgtcga gggtggcggg atcgtagtcc gcagcgtagc cgggcaggat
     3301 gaaatccagt ttggcgaggt tgtcccggtg ttcgctgtcg cgcagttgcg ggtccaggtc
     3361 gtcggcgacg aaggtggcgc cggcgcagcg tacgccgtcc acctccgggc tgacgtaacc
     3421 cagcctgcac accacgatgc cgggcgcgct gccggacgcg gctggcaggt gggagacctg
     3481 tccgcgggca gggcgcacgg gtagcatggc ggcttccggc accgcgcgca ggccgacgcc
     3541 gttggccaga atggcgatcg gcgcgcgggc gatctcgtgg ccgtccgcgt cgagggcggc
     3601 ccattcgccg tcgcgacgtt ccaggcgggc gagggtgcgg ccgaacaagg ggatgatgcg
     3661 tgtgccgccc gccgccagac tggcggcgca caggctgggc ggctggatcc agccaccgcc
     3721 gggaaaccac cagccgccgg cgctcacggg ccagtcggcg aggcgggccg cctcttcctg
     3781 cgtgacgtaa cgcaggtagg cgtcgggggg ctgctgggcc tccacgacgg cacgcatctt
     3841 gttttcctgc ttggcgtcgc gggcgaggtg gagcaccccg gtcggagccc agcgcactgg
     3901 atggccgcgt tcggccagct gggtgagatg ctgccggccg tacagaaagc cggcgcgggt
     3961 gaggcgggcg aggcggttgt cgtccaggct gggctgcggc cggaagaccc ccgcccggtt
     4021 gcccgaggcg ccctggccgg gcgcgtcggc gctgtcgatg agggtgatct gccagtcacg
     4081 ggcagcgagg cgttcggcga ccgatgtacc ggcgacgccg gcgccaatga ccagggcgcg
     4141 gtgggaatcc gggatgccgg tgctcgtcgg ccccggaccg ggaaaacggc ccgcaagcat
     4201 gtggcgcttg ccgccgaagc ccggctgtcg ttcgacgatg aagcccgcct cctgcacgcc
     4261 gcgccgcacg tggccggcga cgcaccaggt ggacagggtg gcgccggggg cggcgaggcg
     4321 cgccagttgg gtaaagacgg cgggggacca catgtccggg ttgcggtcgg gggagaaacc
     4381 gtcgaggtag aaggcgtcgg cccgcatggt cagctcgggc aggacctgaa gggcatcgcc
     4441 cagggccaga ctgaggacca cccgaccttg ctcaaaggtg aatcgatgaa tgcctggcac
     4501 cagattcggc catgcgttca gcaggtgtgc cgcctgcggt gcgagttcgg gccagtcact
     4561 caggacgcgc gccagatcgt gccgcgtgag ggggtgcttc tccacggagg cgaaatgcag
     4621 gtgctgacag cggtctgcat cggctgccca ggcgtgccag gtggcgagaa aattcagacc
     4681 gagtccgaat cccgtttcga gcaccgtgaa ggtccggcgg cccttccagc gcgctgcgag
     4741 ttcgttcccc gcgatgaata cgtggcgggc ctggcccagt ccgcccgagg cagtgtggta
     4801 aacgtcatcg aattgcaggg agtagggtgt gtttgccggg ccgggggtga aacgggcggg
     4861 aatgagggac atggaatcta gctgcagtga agacgtcggg atgctgtgcc tgagtcgtcg
     4921 ggcgcgtgaa ctcaggcgcc ccgcactgca catgggcgcg gggccgggtc gaggaaggcc
     4981 ggggtcgagg atgactggcc aagatgctat actcttcccg ctattctgga taactgtctg
     5041 attttctgtc tgactgcgcc ttttcgggcc ttcccgtcat tttccccacg tgccttgctt
     5101 gccagattca gcgtcgcgcc cgaatacggg cagcctggcg ccggagtccc ccggatcgcc
     5161 ggaagtggcc attctcatgg gctgctattt tgggcaggac tatctggcgg cgcaactcga
     5221 ttcgatccag gcccagtcgt atcgtctctg gaaggtctgg gcggcggacg acggttccac
     5281 cgatgccacg ccggaattgc tcgagcgtta tcaggcgtcc tgggggcgcg accggctggc
     5341 ggtgcgacgg gggccgcaac agggattcgt cgcgaatttc ctggccctgg tctgcgatga
     5401 cgggattcag gccgactatt tcgcatattc ggaccaggat gacgtgtggg cgccggaaaa
     5461 gctcgccaat gccgtggcct ggctggcgtc tgtgccggct ggaataccgg cgctctattg
     5521 ttcccggacg cggctgttca aaggggcgcg gactgttggc tgttcgcccc gacatcggcg
     5581 gccgccgtcc tttgcaaatg cgctggttca gaatattgcg ggtggcaata cgatggtctt
     5641 caaccgggcg gcacgtcgcc tgctggcaca cgccggtccc gacgtgaagg tcgtcgccca
     5701 cgattggtgg acctatattg tcgtgactgc ctgcggcgga caggtgcatt tcgacgaaca
     5761 aatgaccgtt gactatcgcc agcacgagga caatcagatt ggtgccggca atggctgggc
     5821 attcctcgtg cgccgtatcg aaggattctt caacgcgcgc ttccggtgtt gggtggatgg
     5881 caatctcgtg gccctcgagc gcctgggtgc gtgcctttca ccggaaagta agcggatttt
     5941 tgccttgttt tcggccgctc ggaaaggcgg gttgtctgcg aggctttcgg ggttgctgag
     6001 atccggtgta catcggcaga cggctggcgg gaattgcgga ctgctgatcg ccgcactctt
     6061 caacaggatt tagcaatgac ggctcacggg aggcgcgggc ggaagctgag cgatcgcacg
     6121 accggcgctg gcagagcctg agaggtttga atgcagaaat tcacgtccag tcccgcgggg
     6181 atgctcatgg gattctggag tcaccggaat ctcctgatgg ccctggccag gcgcgaggtc
     6241 gtcgggcgct atcggggctc cttcatgggc atcctgtggt ccttcatcca cccggtcttc
     6301 atgcttgccg tctacacctt tgtcttcagc gtgattttcc gggcgcgctg ggggggcggc
     6361 agtgaggggc gggaggagtt tgcgctggtc ctgtttgccg ggctgctggt gttcaacgtg
     6421 ttctcggaat gcgtcacaca ggcgccgggg ctgatcctga gcaacgtcaa ctacgtcaag
     6481 aaggtggtct tccccctcga gatcctgccc ggcgtgaccc ttgtatcggc gctcttccat
     6541 tgcggcatca gctttgtggt atggctggtg gccgacgtcc tgctgttcgg cgtgccgccg
     6601 gtgacggtgc tgctgtttcc gctcgtcctg cttcccttgt gcctgttcgt gatgggcctg
     6661 tcctggctgt tggcgtccct gggcgtgtac gtgcgggatc tcgcccagtt catcgggctg
     6721 gcgacctcgg tgctgatgtt cctgacgccg atcttctacc cggccacggc cctgccggag
     6781 gcgtaccggg cattcctttt cctgaatccg ctcaccccgg tcattgaaca ggcgcgcgac
     6841 gtcctgtgct ggggcaaggg gctcgatggc gcggaatatg ccctgaccct gtgtgcgagc
     6901 gcgctgtttg cgtgggccgg atttgcgtgg ttccagaaga cccggaaggg gtttgccgat
     6961 gtcctctgag gtcgtcatcc gcgtggcggg gctgagcaag tgctatcaca tctacgacaa
     7021 gccccatcac cgcctgatgc agatgctgtc ccggggcagg cgcacgtatt tccgggaatt
     7081 ctgggctttg cgcaacgtct ccttcgaggt gcgcaagggc gaggcggtgg gcatcatcgg
     7141 gcggaacggc tcagggaagt ccaccctcct gcagctcatc tgcggcaccc tgaacccgac
     7201 ctcggggagc gttgaaaccc gtggccggat cgccgccctg ctggagctcg ggtcgggatt
     7261 caacccggag ttcaccgggc gggaaaacgt cttcatgaac ggcgccgtgc tcgggctcac
     7321 gcgggaggag atggaagccc ggatcgccga catcgaggct tttgcggata ttggcgagtt
     7381 catcgaccag cccagcaaga cctattcgag cgggatgctg gtgcggctgg cctttgcggt
     7441 gcaggtgtgc atcgagccgg agatcctgat cgtcgacgaa gcattggcgg tgggcgatgc
     7501 cctgttccag aagcgctgct tcgagcggat ggagcatttg cgcagcaacg ggacgacgct
     7561 gctgttcgtg tcccatgatc aggagtccgt ccgtgcgctg accaatcgcg ccgtcttcct
     7621 tcatcacggg gaggtccagc acaccgggaa ctcggcggac gtgctgctgg aatatcgccg
     7681 gctcctgcac gaggaggaga cgcgcgccct gggcgaggcc gccgcgcgcc tgcgcaaccg
     7741 ggcggcggag cagcgcgctc agcaggccgc ccgcccggtg gcaccggcca cggcggcgag
     7801 ccccgcagcg gtggacgccc cggcgccctc ccgctcggcg gagctgtcct tcggcgacat
     7861 ggacgctgag gtggtgtcgg tgtcggtgtt caatgccggt ggcgagccct gttcccattt
     7921 cgatccgcgg gatgtggcgc aggtcgtcat ccagtgcgtg gcgcggcgcg atctcgaccg
     7981 gctcaatgtg ggtctgcgca tccgcaacaa ggaaggcgtc aagatctact cctggggcac
     8041 gctcaaccag gacatggcga tccgcgccgg gctggcggac ggcgaggtgt tctggaaccg
     8101 ttcgttcaag gcgggagagg tgttttcggt gagcctggat ttcgtcgtcg ggctgggacc
     8161 gaacctgtat gagatccagg ccagcatcac tcaggaaggc aaaccgtatt acgccgagca
     8221 gcgcatgctc cactggatgg acgaggcggc cttcttcacg gtggcgacgg atccgcgcac
     8281 ctaccatttc ggcggcgtgg tggacatgcg catgtccagc cggctcctgg ggggggcttc
     8341 atgagcggaa cgcccgccgg gggaatgacc cccgcgcttg ccgatgcggt gatcgaggag
     8401 gtcctgctcg cctgcggggg cggaacgctg gcggttgtcg gtcagggcgc ggcgcactgc
     8461 gtccgcgccg ccctgcgcat cgggatcgac gcccgggggg tggaggtctc cgatgcgggc
     8521 gcagttgaat gggcgcgaac ggcgatgccg ggtcgcgtcg tggccggctc ggtatgtgcg
     8581 ctcccctacg ccgacggcgc tgtggatacg ctggttgtcc tgcacggcct cgagacgctg
     8641 gacgaagacg acgtcccgac ggccatcgcg gagatcggtc gggtggcgtc ccgcgcggtc
     8701 tatctgagga tggccacatc cccggcgtcc ggtgccgatg gcgtgttccg gtcgcggggc
     8761 gggtgggagc agccactgtt cagtgccggt ttccgcaagc acccgtctta ctaccgcgtc
     8821 aatgcctacg aggcgctgga gggggaggcg ggcgaaatca ccatcgtcct ggaaaagctg
     8881 cccgcgcccg cgctgaaacg ctatccgctg gccgcgctgg cggaagagcg cgatctgcat
     8941 atggacatgc tgcgcgagac gggcgcccgc tcggacgccc acgtggcccg ctatcagtgg
     9001 gctgcggcct tcgtgcgccc cggcgacacc gtgctcgacg cagcctgcgg cctgggctat
     9061 ggcagctatt tcctgcagac cgggaccgag gcggcgtgca ccctcggtat cgacggcagc
     9121 gcgtacgcga tcgattacgc ccgtgagaac ttttcagcga tcgtgccggg cctggagttt
     9181 cgcgaaggct tcctgccggg cgcgctcgca tcgatccccg atcattctgt cgacgtggtg
     9241 gtgtccttcg agacgctgga gcacgtggat gacaatctcg cactcctcgc cgagtttcac
     9301 cgcgtcctga cccctgccgg acgcatcgtc gtcagcgtgc ccaacgactg gagcgatgaa
     9361 accggcgagg acccgaatcc cttccacgtg cacgtctaca cactggagcg cctgcgccgg
     9421 gaattggggg cacatttcat cctcgaaaag ctcgtggccc agtcggccag ccagcacaag
     9481 agcggccccg accgcacggc ctggacggcg gccgggcgct cgctgcggga cgtatcgccg
     9541 gacgtggccg acgatcaggc ccccgacgcg gaatggtggc ttgccgtggc gatgcgttcg
     9601 cccctggagg ggcaggacgt gccgtaccgg gagacccagt tcccgcagtt tccgtcgccg
     9661 gactggaacg ttacggcatt cgggcgggat taccggaatc cctggctggt ccggggcatg
     9721 gtggatatcg cccatcggct tcaggcgccc ggggcattgc acgtgctcgc cgaagaagtg
     9781 gccgcgacgg ccgccctcga taccgccgac gcgggcgctg ccttatgcgt gctggcctac
     9841 cagatgctgg atgcaccgga aaccacgtgc gccgccgtgg aggctctgga ccgtcgcgtg
     9901 atggtctacc tggacgagga tccccccagt ccgcacggcc agcgttggtg cgtgtccttg
     9961 cagttcgtgc tggggcggct gtggatgggc gtgggtgatt tcgcccgtgc ggccgaatcc
    10021 ctggaaggct gcatcgcgcg ggggtcggat cgcttcagtc cgctgctgga caaccgggtg
    10081 gtggaagcct gcctcattct tgggcagttg aacctgcacc aagggcatcg cgactccgca
    10141 ctgcgatggt ggtccaccgg gatcgagcgg gcccgcaggg cggtgggggg ggattggtca
    10201 ggcgcgatcg gcgatctggc ccatccggcc gaattcggct tgccggaact ggcgtcgatt
    10261 ctcgaatatg ccagtgcgtg cgcctatgcg ctcgtccatg tcgatgacgt ggacgtcaag
    10321 ccctggtggt ggctgcatcc ccggcgcgac cgtctgtccc ggcacgccgc cgtgttgcgc
    10381 gagctggtct gtcagcgcga gcaactggcg gtccgtgatc gggagctggc tgcctaccgt
    10441 gcccagtcgg atgcataccg cgagcaactg tcgcgggccg tcgacgccgc gcgcgacacg
    10501 gcggcctaca gcgaacgtct ggcgggagaa ctgggaagct atcagggtca gtccgatgcc
    10561 taccgtcagc aactggacgc ggcccagacg acgacgcgcg agctttcagg gtatgcggaa
    10621 cggctggtcc gggagctggc tgcgctgaag gcagagtcgg aagaatacgc ccgcagcgtc
    10681 cgcaaggcgc aggccgatgt catcgacgcg acggcctccc gggcggaggt ggagcgcgcc
    10741 ttgagtgaga gccggaacgt cgctgccacg ctcgaacggc ggcaggccga ggccgaggcc
    10801 cggctgagcg cggctgatcg tctggttgcg ggcttgcagg gcgaggtggc ggcctatgcg
    10861 cgggagtccg cgtcgtacgc ggagcagatc caggctgccg aggcgcgcct tcaggaaagc
    10921 gctgcgtacg cttcaacgct cctgagccag atcgaggcct accagcgtca gtcggtcgag
    10981 tattgcgagc agatccgggg cgccgaaacg cagctccagg cggcgtccga gcacatcgcg
    11041 agcctgcagg atcaggtcgt ggcgtacgag cggcaggcat cgcagcacgc cgaacagagc
    11101 cgggccgcgg aggcgcgcct tcaggaaggc gctgcgtacg cttcaacgct cctgagccag
    11161 atcgaggcct accagcgtca gtcggtcgag tattgcgaac agatccgggg tgccgaaacg
    11221 cagctccagg cggcgtccga gcacaccgcg cgcctgcttg agcatgtggc cgcgtgcgcg
    11281 ggcgagtcac aggcttaccg gacccagctt gatgaggccg tctcgcgcct gagcgagttt
    11341 gacgcctatg tccggcggct ggaagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnatga ggccgtctcg cgcctgagcg agtttgacgc ctatgtccgg cggctggaag
    11521 ctgaattgac ggcctatcag acccaggccg atgtccatga gaaacggctt gccgcagttc
    11581 aacaggacgc ggacagtgcc gcggcatccg tccggaatct tgagcgtgat gccgtcagga
    11641 tgcgcgatca actggatcgt gaggcccgac ggctgggtgt ctccgaggcg acgcgcacgg
    11701 cgctcgaacg cagtctggcc gatgccgaag ggagggttgc cacattcatc gagcaagaaa
    11761 tggtgcaccg cagtagaatc ggggcgctgg aaagtcaggt cgagtgcctg gagcaggaac
    11821 gaaatgccca tcaggttcaa tatcgatcca tgctgacgct gtattccagc cggatggcaa
    11881 gtccccgttt cctgttgcgc cgtttgtggc acctgatcct ggtgcgggta ggcctgatcc
    11941 gccgtcgtga gagtgaagaa caatgaaaat agtattgact gtccatcagt ttccgccgga
    12001 atacatatcc gggaccgaag tcctgacctt cagcgtcgcc aaggatctga tgcgtcgcgg
    12061 ccatgaagtc cgcgtgctga cgggggcccc tgccaaggca cccctgcggg acgccgaacg
    12121 cttcgatgaa tacgagatcg agggaatccg ggtctatcgg ttcacacatg aattcgtgcc
    12181 gatgggtgac cagaacgtga ttatggaaat ggagtacacc aatcgcctgg tggcccgcta
    12241 tttccagtcc ttactggacg aatttcagcc cgatctggtg catatcttcc acatgtcccg
    12301 cctgggaacg tcgatcatcg acgtggccac cgggatgaag attcccacct acttcacgcc
    12361 caccgatttc tggagcgcct gtccgaccag cctgctcatg ctggacgacg gcaccccgtg
    12421 caaggggccc gagggggccg gcggcaattg cgtgcgccat gtggccatgc gggcgcgctg
    12481 gagccggatc gccaaagctg ccaagtatgc cccggatttc cttgcggatc aggtcgtcac
    12541 gctcgcgcgc aagggttggt tgccgcgaca aatgccgttc cgcaacgaga tctccgcgct
    12601 gtcgaggcgc catgagttcg tgatctcccg tctcaacgcg ctcaatggca ttgtgtcgcc
    12661 caccaatctg atgaccgatg cgctgacgcg taacggggtg cgtcccgagt tgatccggca
    12721 gcacatgtac gggatcaaca tcgacaatta caacgacgtg atccgcgccc atgtgcaggg
    12781 caggacgttg acattcggat tcatcggcgg gctgtcatcc cataagggct gccacgttct
    12841 gatcaaggcg atccggaagc tgacccgtgc ggatgtccgg ttgaagatct acggcaatcc
    12901 gaaggattct ccggcttact ttgccgagct gcagtcactc gccgacgggg ctccgttcat
    12961 tgagttctgc gggacgttcc cgaatggcaa gatcgcggag gtcctgagtg gaatcgacgc
    13021 cttggtggtt ccgtccgtct ggttcgagaa cgcgcccctc gtgattcatt cggcgctggc
    13081 ggcccggtgc ccggtggtgg cgtccaattt tcccggcatg tcggaagtcg tccggcacgg
    13141 tgaaaacgga ttgctgttcg agccgggcaa tccggacagt ctggcggaca cgctgcgccg
    13201 tgtcctggag gagccgacgc ttctgagcga gctcagcagc cggtgccggc cgccgaagtc
    13261 gattgccgaa tatgtggatg aaatcctggc cgtctatgct tcgcctcctg cgcctgaagg
    13321 cgggcggggc atcgtggaat tccgccgctt cgagcccatg gatccacgca atgaggccag
    13381 ttttgtctct ggctggtgca ttgcgaaggc cggtgagccg atgtctgtcg agattcgcgt
    13441 caatgaccgg gtggtggccc aaacccgtca gttcgcgccg aggcccgatg tacgggcagg
    13501 attcgggggc gcaggactcg tgattcgggg tgagcaattc ggctttgcgc tcagttttcc
    13561 ggcgggtatc cagcggatca atgccagtgt gcggattgcc gcacccgacg gacagacgcg
    13621 ggatctgtcc atgtccgagc tcgctgcggg cagcatggtc aaggtgggcg atgatttctt
    13681 catcggcctg gaccaggagt gtttttcctg agcaatcagg gtgattgcgg gctcggagga
    13741 ttttcgccgg tttatataag gggttgtggc ttgaaagtgc agggatactc gttggacggc
    13801 aagccgtggc tgcaggcctt gttttttgtg ctctgtgccg gcgtttacct gttcttgatg
    13861 aacgctcaca aggcggcaga gctcgcggac gatggcgcat ttttcctgcg ttatgcagtg
    13921 aatatgctgc acggacattt ctgggtctgg aacatcggtg aggcgccggt gtggggtgcg
    13981 agcgcgcccc ttttcccggt cttcctcacc cttcccctga tgtttggctt cgatcccgtg
    14041 acgacggtga tgaatgccgg aatcgtggct ggagcggcgg gcctgtcagt gatcgcctgg
    14101 ctgctggcga aacgcatcag tctggtggcc ggtctggggt ttctcgcttt cagtgccacc
    14161 agcagccaga tcacctactg ggtgggctca ggcctggaga cgccactcac gatcattctt
    14221 ctggcctttg cacttttttc gatttttgga accgatcggc catggatcgc aggtgtggct
    14281 gccgggctcc tcatgatcca caagctcgac atggtgcctg tcggactgct cgtcctggct
    14341 gcggcgtggc tgcgggatcg gcgtgttcca gccacggcgg cggtcatcgc gcttctcctc
    14401 agcggggcgt ggtatctgtt tgcgtggtgg tatttcgggg cgccggtacc gaattccttc
    14461 attacgaagg ccttgcacca gagccacctg gtcccgatca tcgactggcg atggtatggc
    14521 cagttcgtgc tgctgagcgg tatcaacaaa tacgcgagcg tctgcctgct cggacttgcg
    14581 tgggcgatca ggccctcacg gcccgtcctg tgcctgttcg gggggctcct gctcattcat
    14641 ctcaccgcct atacgatcaa acaccccgtc gagccttacg cctggtacgg gatgggcgcg
    14701 atgacttcgc tcttcgtgct ggcatcggtc ggactggacg gggttgcgcg acagcttgcc
    14761 aggctccggt cgggtcatca gggggccctc ttgggggacg cggtggtgct ggtgttcctt
    14821 gtgacggcgg tcctggcggt acagcgtcac gagcgaggcc tgtcagaggg tttcaaggtc
    14881 tgggccgaca actatgagcg ggaccgtgcg gatgccgggc gatgggtggc tgcacatacg
    14941 ccggaaagct atgtcgtgat ggcagggcat ggcaaccccg cctatttctc cgggcgtaag
    15001 gtgatcgaca gctcctacct caaccggcga tacgaggccg ggaacgtgct ggaacgtctt
    15061 cgccccgaaa tctacatctc gccggggaat ccatctgacc cggatttctc gaagccgctc
    15121 ttcagtgatt atcaggtcgt caagatattc gacaggacgt tctcgagcgg ggtggactcc
    15181 aagtactggt ttgcggtgct cgcccgcaag gacgcggtcg ggcggatgtc cggcctgaat
    15241 gccggtccga ctgccccggt gtcggtggag aagtatgtgt cggatgtttc cttgggtgac
    15301 cagtttggtg tgctgaggtc ggagggcagt gacacgctgt tcatccatcc cggcgcgacg
    15361 acacccacag gtttcgattt cgatgccagc ggctttgccg ccgggtacgg tggtcgagcc
    15421 ctccaggtga cggtttcagt gtccccgaag gtgcctccgg aggccgttgc gcggggcgcg
    15481 gctgtcgcca gggtgacgtt ctcgaggaag ggtgaggtgg cggcgcaatc catcgtcaaa
    15541 gtgggatccc cctttcattt cacgatgccc gcaaactcgg gatggcgggt ggtcgtcgac
    15601 aattacaacg gtccggatac ggactggatc ttgatttcgt tcaattaggt gaggagtctt
    15661 gaaatgcgtc tgaagaatct taagagtctt gtcgtggccg gctgtttcgt cgcagggctg
    15721 gtggcttgtg aatccggtcc gaacgttccg gcgggcgagg aaagcaagtt tctttttgca
    15781 ctgaagacgt ccgaaaccat cggcgatcag gtcgggaatg ttgcgctgaa tgaaaatggg
    15841 atcatgatcc atccgggcgc cactgcgccc acgtctgtca gcttcaattt ggacgggaaa
    15901 ttgcgtgcca tcgggatcaa gccttacatt gcgcccctta atgaagaagg caaggcgcat
    15961 gccgatgcag gtattgtcgg cgtagaattg ctggtcgatg gaaaatccgt cgataaattc
    16021 acggtcgatc gcaacttcag ccaggaaaag acccttgatg taaagggcgc gaagtcgctt
    16081 gaaattcgtg tcgacaatgg aaacggcacg ccgatctggg actggttcca tgtaaaggtg
    16141 acgtccgtca agtaatcagg tctttggttc cctgatttcg ggtccgtatt tgaggtggaa
    16201 gatgcgttgt ctggtaattg gcgggcgcgg gttcataggg tcgcatctgg tggatgcgtt
    16261 gctggacaag ggatatgcgg tcaggtgttt tgaccgccct catgtggcac cgctggggga
    16321 ttcgcatctg tcgaaccccc gcttcgagtt ggtggaggga gaccttgtca gcgaggcgga
    16381 cgtcaccgag gcgctggagg ggtgcgattt ctgtttccat ctcgtgtcga cgaccctgcc
    16441 caagtcgtcc aatgcggacc cggtgtttga cgtggagagc aacgtcctgg ggacagtccg
    16501 gctgctttcc cacgccgtca agagcggcgt gaagaaggtt gtgttcgtgt cgtcgggggg
    16561 gacggtctaa gggattcccc gtagcgtgcc gatccgcgag gaccatccga ccgatcccct
    16621 gtgttcgtat gggatcacga agctggccat cgagaagtac ctgggcctgt tccgtgaatt
    16681 gcacggtctg caatgcgcgg tcctgcgact ggcgaacccc tttggcgagc ggcagcgcac
    16741 gcatgccagt cagggggccg tcgcggtctt tctgggcaag gccctgcgtg gcgagaccat
    16801 cgagatctgg ggcgacgggt cggtcgtacg ggattacatc cacatcgatg acgtcgtctc
    16861 ggccctgttg gctgcgctcg aaaagacgac tcgtgagcat gtcttcaaca tcggctcggg
    16921 acgcggtctc agtctgaatg aggtgctcga caccatcgag cgggtgaccg gtcagcagac
    16981 gcagcgcaac tatctgccgg ggcgggcgtt tgacgtgcca gtcagccagt tgtgcatcga
    17041 acgggcccgg gcagagctcg agtgggagcc ggtcatccat ttcgaggacg gcatccggcg
    17101 tttcgcccgc tggatcgagg cggacgagct ggaaggcgcc cgataggggg cgtgagtgcc
    17161 ggtagtccgg cgcatggcgt cctggcggaa tcgctgccgg cgcgggggcg ccgcgcttga
    17221 gcgccgatcc ttgcagcgtt ctgcccgctg gccgacgaat ttttgcgggt caggcgttca
    17281 gcagcggtga ctccttgatt tcttccatca ccacgtagct gcgggattcg accacgggca
    17341 gtctttcgac caggtcggcg atgaacgggc ggtattcgcc cagattcggc aggcgcgcct
    17401 tgacgaggca gtcaaaatct ccggacacgt agtgacattc catgatgtcg gggatttctg
    17461 cgagtgtttc gctcatctgg gtcagggcct ctgccgatcg agacgtgagg cgcagttcca
    17521 ggaagagcag ggcgttcagg cccagcgcgc gggggttgag acgggcgtga tagccggcga
    17581 tgacgccgtc ccgttccatt cggcggacgc gctccgtgca gggcgtgacg gacaggccga
    17641 cctggggcgc caggtcggtc atggtgatgc ggccatcccg ctgcaggatt ccgaggattt
    17701 tccggtcgat ctcgtcgaag ttgcgcatgg gtctgtgtcc ttctcggtca gcttgaagtg
    17761 gtgaagggaa cgcggagacg cgccggcctg tgccgtcgcg tctccgccgc gtgcgctgga
    17821 gcatgggtct ccagaggcaa ggtcagttgc ggagcggttc gcgcttgccc ggctggccgc
    17881 catcccttac cgggcgggtc atgcgggtct cgaagtcggc cttcagctcg tctgccttct
    17941 tcttctgttc cggcgtcagc acctggtaga tctggttgcc ggtgcgcaga cgcatctggg
    18001 tgagctcggc catggcgcgg gcttcggtgt cgctgagggc cgcgacgcgg gcctcgtcat
    18061 acctggccga gaaagccatg tcgcgcagct cttcgtgcgc cttgcgggcg gcgatggcct
    18121 tgtcgcggaa ggccggtgcc tgctcgtgca tgatcttgaa gatctggtcg cgctgggcat
    18181 cggagagctt gagttcgcgg acgaagggcg gcaggtgcat ggcgccgcct tcggggccac
    18241 cgaaacccat ttcacggtgc atcatcgggc cgccgcagtg ttcgtggccg ccgaaggcgt
    18301 gggcggcaaa gggggtcgcg accgcgatac cggccacggc gagtagggtc ttgaagcgat
    18361 tcgtgaagag cgaggacatg atggactcct gtaagtcgtc ggaaggtgcg tccaggggcc
    18421 ggagcttcgg cgggcctgac ggatcacatc ttcctttttc agccggtaaa acgcggtcag
    18481 cgcggggtaa agctgtgtaa gcgtccgccg gaagggtctt tacctccgtc gcggccatcc
    18541 ttgcgcaact cgcgctgctg ggcctgcagt tcgcgcaggc gggcgtcggc gatggtcaga
    18601 tcccgctcac tgccgtcggc gctgcgtagc gcgatgcggt attgctcgat ggcagcctgg
    18661 gtgttgccgc gggcagcttc aaattccccc tgatgaacgt gggagcgggt ctgctggccg
    18721 agggcggcga aggcttcggc ggcgaggcgg tgcagggtgg ggtcctgcgg gcgggcaagc
    18781 aggcggcttt cggtgaagcg ggccgcggca gcttcgtcgt tcaggcggat cagcacgcgc
    18841 ggatagtcgt agaccagctg caggtgatcc gggaagcgct gcagggccga ttcgtagcgt
    18901 ttgcgggctt cgggcaggcg gccggactgg agcagtatct ggccggcgag ggcttccacc
    18961 atggggtggg ggccgccccg ttccacgacc gcgagctcct tgtcggcgcg cttgaaatcg
    19021 cccgcccgca gcagggcggc ggtgagcccg tagcgggtgg cgcgggcatt ctgggtgcgg
    19081 ccctcggcga gcgccttgtc gagcagggcg acggcctcgc ggggctcacc ctcgtagctg
    19141 cgcagcaggg cgcggacgaa gtggtattcg tcgctgtccc gcacctgctt gtagctcagg
    19201 ttgaaggccc ggtcctcggc ctcggcaatc cgttcgtggg tgagggggtg ggtgcgcagg
    19261 tagctgggga cattgccgtc cacgaggcgc gtggcctttt gcaggcggtc gaagaagctg
    19321 gccatggcgc gggggtcgaa gccggcgccg gtgaggagct ggaagccgat gcggtcggc
//
LOCUS       DAOQPA010000066        19345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1414_length_19345_cov_18.530430, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000066 DAOQPA010000000
VERSION     DAOQPA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_19345_cov_18.530430"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3345)
                     /locus_tag="PKX01_10390"
     CDS             complement(<1..3345)
                     /locus_tag="PKX01_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HMZ76578.1"
                     /translation="MTPFTLTFYSYKGGVGRSILAANVAALLARQGKTLLWDLDIEAP
                     GLHRVGDLASGKPNEAGFFEWLLDWQTRSRFDSPNAAACRALSACLLPVERQPRLHLL
                     PAHGADAPFPKLYQQIEWQRFLAEEPARGLELFRSLIDWFGSDEGGAFEHIVLDSRTG
                     ITDIGGLLAALLPHVTVLVGNYGAQNTGGLKEIWKALQLQVGNRDPERTLPPLRLELV
                     ASPITPDDAETAAAQRRAWTDYFSLAAANLIEIPESPRLRKRESVLALTQPDTADDPL
                     LAAYQRLTQRLHAIRSEQEAETRAAEQADFERLDDPRSARHPSNAAKGRRFEDTTAHL
                     LRMLGYQVEPEQSLDGKRIDLVATLRQGLEHNTYLVECKDHKAAVGVEIVEKMSGWVQ
                     HLPKARALGARGMIVARSFSPAALEFAGANNIRCFTHDDLERALIDFSPYLNRLVSEF
                     AASPLASAYVTQRVIPEHNPDTPEELLAYGLRWVRGEGSRLWVLLGDYGTGKTAFTRR
                     FAFELARIALQEDGDTPYPLLINLRDWSSKTSLADVLHDHWHARTGERRDPAIFLHLL
                     ARGRIVLLLDSFDELGVAQAHRNVVEQFRGLVSATAQEGDRLGANRILVTCREQFFRD
                     REDVVKAAAGHSDSLAPLEQAARSFAGRIDLLARFEDAQIRDYLAKRLGAAAGQAAWA
                     QIDAIYDLKSLADRPQLIEIIIGSLPRLAAGGRAVTAGALYLEYTNAWLDDPAIRPAE
                     RQSNSDELRCILETLSIELWRREGQQIHYRDLAQLVGDPSVRGRHDPVNLDVELRTAA
                     FLSRSADGHYRFSHRSFLEFFLARALLRAVKTQCFADALAVPPLSAEVCAFVADLMSA
                     WAMGEEVAAANRAVLLADYRPHASENALRLGYRMTKTDDQNEWLPVGAHLAGAQLAYE
                     AFDGLKLPNADLRGADLFGAQLDKANLEGAQLDKARLDQAFMGLAILNKASLKGASLQ
                     QSDLRHARLFAAHFGAAHLEGANLRGIRGHGSRWQEASLRDANLSGADLSGADFTDAD
                     LCRAVLLRADVTAWQAPRQFNDAGFVPGSLRHTNPGGLMPLDLPGHSNELTACAFSPD
                     GRFVLTASADNTARLWDAHSAQELRRF"
     gene            complement(3345..3968)
                     /locus_tag="PKX01_10395"
     CDS             complement(3345..3968)
                     /locus_tag="PKX01_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76579.1"
                     /translation="MLADLFLAPDPTRVADTATVQDVLDALRACTAEDGWPAEARDVL
                     DFVLTVWVADSTARPNDREGVAELQRLVRYALERARNRHAVPEAFRTRWQGFFDVLEA
                     RRRTLVGRDPALVLDRRHVRELLAAIGHAGSTQQAVTEAFKSQGKELTSGRLSQLLTL
                     LEAHGLITRQRTGRENRLMLTEAGRPHVQAAQPASPSKRGFANKLVA"
     gene            complement(4071..5210)
                     /locus_tag="PKX01_10400"
     CDS             complement(4071..5210)
                     /locus_tag="PKX01_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76580.1"
                     /translation="MEPEFVKKRLDNERWPDIRELESHPLDWLRGLDEYVHRHTRFHR
                     TVSLTDGDARFHLIRHVHPHSYPSYRDRQRGNLQYHLRHHRFVPCALHGHPVALQAVS
                     DVCEQATAAILQRQRLVVAVARFDDGVTEEINWNHGNGHFHMTGLSDAPTRRRSIEAA
                     LDFARQHHADLLVFPELTIPADLRDDIRDDLQARASSVGKESDASALPALIALGSFHE
                     QDATHQHLNRALLVDRFGDVLLTADKRAKVTIAHQGRDWGEALQVAPTPCSLLPLDIG
                     LLALAICKDVFDGPVSRQLQDVGLDWLLAPSMTDHLGPHRTNTGNLLRAHGTVSIVAN
                     QPMPGCTRSGDAFPCGYVDRPDGPTECANLLTLVEIPLSIRLDQA"
     gene            5512..7074
                     /gene="guaA"
                     /locus_tag="PKX01_10405"
     CDS             5512..7074
                     /gene="guaA"
                     /locus_tag="PKX01_10405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMZ76581.1"
                     /translation="MHKKILILDFGSQVTQLIARRVREQQVYCEIHPYDVSEDFIRGF
                     APVGIILSGGPNSVYAAEEVKAPQVVFELGVPVLGICYGMQTMAAQLGGKVESSGKRE
                     FGYAEIRARGHSALFRGLEDRSNADGHGLLDVWMSHGDKVTELPPGFKVIASNDSTPL
                     AAMADENRGFYAVQFHPEVTHTLKGREMIARFVHDICGCTHDWNMPDYVNEAIEKVRA
                     QVGTEEVILGLSGGVDSSVVAALLHRAIGDQLTCVFVDNGLLRLNEAEQVMDTFARNL
                     GVKVIHVDASAQFLGHLAGVTDPEAKRKIIGREFVEVFQAEAKKLPNARWLAQGTIYP
                     DVIESASAKTGKAHAIKSHHNVGGLPETLNLRLLEPLRELFKDEVRELGVALGLPHAM
                     VYRHPFPGPGLGVRILGEVKKDFADLLRRADAIFIDELRAADWYDKTSQAFAVFLPVK
                     SVGVMGDGRTYEYVVALRAVETSDFMTARWAELPHSLLGKVSNRIINEVRGINRVVYD
                     ISGKPPATIEWE"
     gene            7415..8611
                     /locus_tag="PKX01_10410"
     CDS             7415..8611
                     /locus_tag="PKX01_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ76582.1"
                     /translation="MLSDTKLRNLKPQGKMYKVADRDGLYVAVTQAGAISFRYNYTLN
                     GRQETVTFGQYGIGGITLAEARERLGEAKKLLAAGKSPAREKARAKDREKDAKTFDDW
                     AQGWLRGYEMADSTRDMRRACYERELKGPFGNKLLTEITHDDLRRLTDKIVERGAPAT
                     AVHAREVLMQIYRWANERGQRVDNPADMVRPRAIAKFQPRERALSPDEIGLMYEYLEK
                     VAAGPQFKVACKLLLLTMVRKGELTDAKWSEISFTDALWTIPKERMKRRTPHLVFLSR
                     QALDIFIALKTFAGGSEWVLPSRYDAGQPMSNATLNRVLELVYTQAQKDGKDLGKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQRGVRAVYNKAEYREQRTAMLQDWADMIDG
                     WTIKKR"
     gene            complement(8598..8852)
                     /locus_tag="PKX01_10415"
     CDS             complement(8598..8852)
                     /locus_tag="PKX01_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HMZ76583.1"
                     /translation="MRTIQDNELKTPGLVLISRRQLLSMVPLSDRTIYNMEKRGDFPK
                     RIALTSRNVAWDLGEVKAWIEGRKVSGGQAVRPGQTVIAS"
     gene            9334..10140
                     /locus_tag="PKX01_10420"
     CDS             9334..10140
                     /locus_tag="PKX01_10420"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMZ76584.1"
                     /translation="MLDFSDASFSDFFASELKVDINDSKYAVNGGSKGKRLRCFLQSC
                     NDATAVRTLTALWEHRSECLARTGGKDPVINAEARYQALINRLSGGAPPQPAAANPTA
                     SAVDRQNLAKIKADLLQVTSLSPQARGYAFEGFLKSLFDAFGLAAHEPFRLRGEQIDG
                     SFELGSDIYLLEAKWHGQPIGVGELHTFHGKIEQKAAWTRGIFVSNSGFTDDGLAAFG
                     RGKRVICMDGLDLYEILEREIPLTQVLERKVRRAAETGSPFVRVRDLFPY"
     gene            10155..10934
                     /locus_tag="PKX01_10425"
     CDS             10155..10934
                     /locus_tag="PKX01_10425"
                     /inference="COORDINATES: protein motif:HMM:NF020452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HMZ76585.1"
                     /translation="MAQFNDWCLSVDEPVGNHFRRVMTGQAGNLPTGIQATAAIVPGH
                     YASEEQVVRAFARLGKPAAAALIEGKLPTTKAIRSGDLGEIYATEWIDAHSGGYRAPI
                     KRLRWKDHRNMAMRGEDVIGILQDAQTQRLHFLKTEAKSRATLTAQVVTEARACLDKD
                     GGLPSAHALSFISARLLELNNLPLADAIDDALLKHDIPVQNVRHLLFTFSGNAPDALL
                     TASLQAYPGPINQWGIGLHVEGHAAFVGAVYDQVIANANNA"
     gene            10918..14430
                     /locus_tag="PKX01_10430"
     CDS             10918..14430
                     /locus_tag="PKX01_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ76586.1"
                     /translation="MPTTPEAIAADIAEAATAGFRGRLIARGQARAIIWRNGELPPDA
                     PAFAQQLSYDLHSYGYALLGLGLRLRELGGDTAQARTAFEQAATALEAVIAKGNRQEA
                     DRDFHFIMAAASYHLAHLSARAYSLLAIVEADENFSPIERVLAQLMRRNFRALRVSVL
                     DYRASGEGSDAQIAAAIQANLDQAEAAAEPADGSNDFLFDGLDIALTDAFMAAMSLFL
                     LALERGEQPLLDQALERLRTSLAICSELNMLPQWWAHRIAIHLLSDLWSSTFHENVPL
                     QPAGGEAVDWPRLRELFIALLQQRPKAEVDLWPSQIEAASRAVDQSDDLVVSLPTSAG
                     KTRIAELCILRCLAGGKRVVFVTPLRALSAQTETTLQRTFGPLGKTISALYGSIGVSG
                     FDEDAIRERDIVVATPEKLDFALRNDSSLLDDVGLLVFDEGHMIGLNEREVRYEVQIQ
                     RLLRRADAHERRIVCLSAILPDGDQLDDFAGWLRRDHPGAVIRHDWRPTRLWFGEVVW
                     SSPNARLNLRIGDERPWVQRFLTGAVPPNWVPPKRRRTKLFPCDQRELCLATAWRLVE
                     DGQTVLIFCPERRSVEPFADVIVDLHERGALRSLLESDPAVLNTAIALGEEWLGPDSA
                     IIKCLRLGVALHHGALPTAYRKEVERLLRDNILKVTISSPTLAQGLNLSATAVVMHSL
                     HRFGERIEISEFKNVIGRAGRAYVDVEGTVLFPMFDGIAKKRSNWEALINDLGARDME
                     SGLVRLVAALLSRMGARIGGDLDQLVEYVVNNAAAWTFPEIANEKPEDRERALADWER
                     HIATLDTAILSLIGENDIPDDGIEAALDDILQSSLWHRRLLRQNEQVQQVLKAGLVSR
                     SRHIWNQSTAARRRGYFLAGVGLTTGHALDAIAADANLLLVQANAGILENDSEATITA
                     IMSIAERVFAFYPFTPDPMPTNWRDILRAWLLGQPLATIAAGHESDTLQFIEGGLVYR
                     LPWAMEAIRVRAAANGDTIGDFLLDDHELGLAVPAVETGTLNRSASILIQAGFNSRLA
                     AIKAVTDTGATIRTGQELRQWLNSQAVAALSAQPDWPTAETKVMWTEFVQSFIPRENK
                     TWADRRYRANVAWLGVPPPPDTPVQIHEWAGQPRVLSVDGAPLGTVQAALNHARAGLV
                     RAQVAQDLSKIDITYLGPDDLSVA"
     gene            complement(14698..15315)
                     /locus_tag="PKX01_10435"
     CDS             complement(14698..15315)
                     /locus_tag="PKX01_10435"
                     /inference="COORDINATES: protein motif:HMM:NF020474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HMZ76587.1"
                     /translation="MPGAARDNAKSHPYFTQKVKLHSLHAQQAFDRGFELCANAIFSL
                     SVVLRIIGTDEQAHEVEGLVDERLNKMFEDMRNEASRLDKMAEANGIEFKGIDYSHPK
                     EVEAKITSPRAVRYVGLIREFDGLIAKLDTLWLSGVIADSAYSRSIYEWKRRLLRLAG
                     GIRSIAGRAMIAARRKDGQGDAKDVPDAAASAGDEVAAAVPAPAV"
     gene            15798..16148
                     /locus_tag="PKX01_10440"
     CDS             15798..16148
                     /locus_tag="PKX01_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76588.1"
                     /translation="MEKVLLVMALLGSAHLAFRGVGKVGKLVIAFAAPKSGAVSSVVR
                     MPEVRPMAVRMDGEEDWSKYDIPAFIRRGIPMPTLEPMPAKETKTRKRRSKVKAEGAA
                     TPASSDTATFEVVA"
     gene            17037..17873
                     /locus_tag="PKX01_10445"
     CDS             17037..17873
                     /locus_tag="PKX01_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HMZ76589.1"
                     /translation="MKMENISLLSMPADHYHLHPAVGHSGLVRIMRSPAHYREYATNP
                     PEPTPAMQLGTAFHTALLEPDRFGETFVVATKFDRRTKDGKAAAEAWEADNAGKTALT
                     AEQLEAIRSMAYLVRCHAGADRLLSHGLAEMSGFWIDPESGVECKCRPDFLAMDGDRI
                     TGIVDVKTCCDAGTDGFARAIATLGYDVQAAFYQDGFKTLTGRTIPFYFIAVEKDAPY
                     AVAAYKASDEVIEVGRAKYRGGLQLLKWCRERDIWPGYQPSGEIETINLPRWAAHFDL
                     EA"
     gene            18081..19202
                     /locus_tag="PKX01_10450"
     CDS             18081..19202
                     /locus_tag="PKX01_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HMZ76590.1"
                     /translation="MAALQALKNIKRDTPAEGQPQGFPAMLEKFKGEIARALPRHINP
                     DRMARIALTAFRMTPKLAECDPRSVFAAVIQSSQLGLEVGLMGEAHLVPFKGQCQLIP
                     GYTGLMKLARQSGLVQDIYAHEVRVNDKFLLKLGMERSLEHEPLTAPGGFPASDEERG
                     EVAGFYAVAVFKDGSRTFVAMSRKEVEKIRDGSRGYQSAKQYKKESVWDSDFVAMGLK
                     TVIRRLSKFLPKSPELATALAMDAANEQGRAQNLDLDDVIEGNYAPVADETGDVDADG
                     KEDAKPERAPQETKAEAAGAQATKVESHGATASVIEPGKDGKGNPKLDAAIKAMEKAK
                     TVDALDEAYIRAEAEFEGADLESLMRAYQRIKARIATLI"
BASE COUNT         3532 a         5948 c         6436 g         3429 t
ORIGIN      
        1 gaagcgtcgg agttcctgag cggagtgggc atcccagagt ctggcggtgt tgtcggcgga
       61 ggcggtgagg acgaagcgac cgtcggggga gaaggcgcag gcggtgagct cgttggaatg
      121 accgggcaga tcaagaggca tcaagccgcc cggattggtg tgccggaggc taccggggac
      181 gaaccctgca tcgttgaatt gacgcggtgc ctgccaagcc gtcacgtcgg cccgtaacag
      241 caccgcgcgg caaagatccg catccgtaaa atccgccccg ctcaaatcgg cgccgctcaa
      301 attcgcatcc cgcaggctgg cttcttgcca acggctgcca tgaccgcgaa tcccccgcag
      361 attcgcgcct tccaagtggg cagcgccgaa gtgtgcggcg aacaggcgcg catggcgtag
      421 atcggactgc tggagactgg cccccttcag gctggccttg ttaaggatcg ccagccccat
      481 gaaggcctga tccagtcgcg ccttgtctag ttgggccccc tccagattgg ccttgtccaa
      541 ttgagcacca aagaggtccg cgccccgcag atccgcgttc ggcagcttga gtccgtcgaa
      601 tgcttcgtag gcgagctgcg cgccagccag atgagcgccg accggcaacc attcgttctg
      661 gtcatcggtc ttggtcatcc gataccccag gcgcagcgca ttctccgacg catggggccg
      721 atagtcggcc aacagcacgg cccgattggc ggcggcgacc tcttccccca ttgcccaggc
      781 gctcatcaga tccgcgacaa aggcgcacac ctcggcggac aggggcggca cggcgagggc
      841 atcggcaaag cattgggtct tgaccgcccg gagcagggcg cgggcgagga agaattcgag
      901 aaagctgcga tgggaaaaac ggtagtgacc atccgcactg cgggacagga aggcggcggt
      961 gcgcagttcc acgtccagat tgacggggtc gtggcggcct cgcacgcttg ggtcgcccac
     1021 cagttgagcg agatcccggt aatggatctg ctggccttcc cgtcgccaca gttcgatgga
     1081 gagggtttcc aggatgcagc gtagttcgtc gctgttgctt tggcgttcgg cgggacggat
     1141 ggccggatcg tcgagccagg cgttggtgta ttcgagatac agggcgcccg cggtgaccgc
     1201 acgccccccg gcggcgaggc ggggcaggga gccgatgatg atttcgatga gctgggggcg
     1261 gtcggcgagg ctcttgagat cgtagatggc gtcgatctgc gcccacgcgg cctggccggc
     1321 cgcagcgccc aggcgtttgg caaggtagtc gcggatctgg gcgtcttcga agcgggcgag
     1381 gaggtcgatg cgtccggcaa agctgcgggc ggcctgctcc aggggggcga ggctgtcgct
     1441 gtggccggcg gcggctttga ccacgtcttc ccggtcgcgg aagaactgct cgcggcaggt
     1501 gacgaggatg cggttggcac cgagtcggtc cccttcttgg gcggtggcgg agacgagtcc
     1561 gcggaattgc tcgaccacgt tgcggtgggc ctgggcgacg cccagttcgt cgaagctgtc
     1621 gaggagcagg acgatgcgcc cgcgggccag caggtgcagg aagatggcgg ggtcgcggcg
     1681 ttcgccggtg cgggcgtgcc agtggtcgtg caggacgtcc gcgaggctgg tcttggagga
     1741 ccagtcgcgc agattgataa gcagggggta gggagtgtcg ccgtcttcct gaagggcgat
     1801 gcgcgcgagc tcgaaggcga agcggcgggt gaaggccgtt ttgccggtgc cgtagtcgcc
     1861 gagcagtacc cacaggcggc tgccttcgcc gcgtacccag cgcaggccgt aggcgaggag
     1921 ttcttccggc gtatcggggt tgtgctcggg gatgacgcgc tgcgtcacgt aggcgctggc
     1981 aagaggactg gcggcgaact cggagaccag gcgattgagg tagggcgaaa agtcgatgag
     2041 ggcccgttcg agatcgtcgt gggtgaagca gcggatgttg tttgcgccgg cgaattcgag
     2101 ggcggcgggg ctgaagctgc gggcgacgat catgccccgc gccccgagcg cgcgggcctt
     2161 gggcagatgc tgtacccagc cgctcatctt ttcgacgatt tcgacaccca ccgcggcctt
     2221 gtggtccttg cattcgacga ggtaggtgtt gtgttccagc ccctggcgca gggtggccac
     2281 caggtcgatg cgtttgccgt cgaggctctg ttcgggttcg acctgatagc cgagcatgcg
     2341 caggagatgg gcggtggtgt cttcgaagcg ccggcctttg gcggcattgg aaggatggcg
     2401 ggcactccgg gggtcgtcga gacgttcgaa atcggcctgc tcggcggcgc gggtttcggc
     2461 ctcttgctcg ctgcggatcg cgtgaaggcg ttgggtcagc cgttgatagg cggccagcag
     2521 cggatcgtcg gcggtgtcgg gctgggtcag cgcgagcacg ctttcgcgct tgcgcaggcg
     2581 gggggattcg gggatttcga tgagattcgc ggcggcgagg ctgaagtagt cggtccatgc
     2641 ccggcgctgg gcggcggcgg tttctgcatc gtccggggtg atgggcgatg cgaccagttc
     2701 gaggcgcagg ggcggcagcg tgcgctccgg gtcccggttg ccgacctgga gttgcagggc
     2761 tttccagatc tctttgaggc cgccggtgtt ctgggcgccg tagttgccga cgagtacggt
     2821 gacgtggggg agcagggcgg cgagcagtcc gccgatatcg gtgatgccgg tccgcgaatc
     2881 gagcacgatg tgttcgaagg caccgccttc gtcgctgccg aaccagtcga tgaggctccg
     2941 gaacagttca agccctcgcg cgggctcttc ggcaaggaag cgttgccatt cgatctgctg
     3001 gtacagcttg ggaaaaggcg catcggctcc gtgggcaggc agcagatgca ggcgcggctg
     3061 gcgttcgacg ggcagcagac aggcgctcaa ggcacggcag gcggcggcat tgggcgaatc
     3121 gaagcggctt cgggtctgcc aatccagcag ccattcgaag aatccggctt cattgggttt
     3181 gccgctggcc agatcgccca cccgatgcag tccgggcgct tcgatgtcca ggtcccacag
     3241 cagcgttttg ccctggcgag cgaggagggc ggcgacgttg gcggcgagaa tgctgcggcc
     3301 gacgccgccc ttgtagctgt agaaggtgag ggtaaagggc gtcatcaggc caccagcttg
     3361 ttggcaaatc cacgcttgga cggggaggcg ggctgtgcag cctgcacatg gggccggccc
     3421 gcctcggtga gcatcagccg gttctcgcgc ccggtgcgtt ggcgggtgat caggccgtgg
     3481 gcttcgagca gggtgaggag ttgggacaga cggccggagg tgagctcctt gccctggctc
     3541 ttgaaggctt ctgtgacagc ctgctgggta ctgccggcgt gcccgatggc ggccagaagc
     3601 tcgcgcacgt gacgacggtc gaggaccaat gccggatcgc gcccaaccag ggtgcgtcgg
     3661 cgggcttcga gcacatcgaa aaagccttgc cagcgggtgc ggaaggcttc cggcacggcg
     3721 tggcggttgc gggcccgctc cagggcgtaa cggacgaggc gctgcaattc ggcgacgcct
     3781 tcccgatcgt tcggccgcgc ggtgctatcc gcaacccaga cggtgaggac gaaatcaagc
     3841 acatcccgcg cctccgccgg ccagccgtct tcggcggtac aggcacgcaa ggcgtcgagc
     3901 acgtcctgca cggtggcggt atcggcaacg cgggtgggat cgggggcgag gaagaggtcg
     3961 gcgagcatga cgggatggaa tcctaaagcg aactcatggt tttacaacga tacagtttag
     4021 tactgtttag cgacgttgcg agcggttcgt gagtgtgcag cctctgggtg tcaggcctgg
     4081 tcaagtctga tcgacagggg gatctcgacg agggtgagga ggttggcgca ctcggtgggc
     4141 ccgtctggcc gatccacgta gccgcagggg aaggcgtcgc ccgaccgcgt gcagccgggc
     4201 atgggctgat tggcaacgat ggacacggtg ccgtgggcgc gcaggaggtt gccggtgttc
     4261 gtgcggtggg gaccgaggtg gtcggtcatg ctcggagcga gcagccagtc cagcccgaca
     4321 tcctgcaact ggcggctgac ggggccgtcg aagacgtcct tgcagatggc cagggccagc
     4381 aggccgatgt cgaggggcag caggctgcag ggggtgggtg ccacctgcag ggcttcgccc
     4441 cagtcacggc cctgatgagc gatggtgacc ttggcccgct tgtcggcggt gagcaggaca
     4501 tcgccaaaac gatccacgag cagggcgcgg ttgagatgct ggtgcgtggc gtcctgctcg
     4561 tgaaaactgc cgagggcgat gagtgcgggg agggccgatg cgtcggattc tttgcccacc
     4621 gacgatgcgc gagcctgcag gtcgtcccgg atgtcgtcgc gcagatcggc cgggatggtg
     4681 agttcgggaa agacgagcag atcggcgtgg tgctgacggg cgaagtcgag ggcagcctcg
     4741 atggaccgcc gccgggtcgg cgcgtcgctc aggcctgtca tgtgaaagtg gccgttgccg
     4801 tggttccagt tgatttcttc ggtcacgcca tcgtcaaagc gcgccacggc cacgacgagg
     4861 cgttgccgtt gaaggatggc tgcggtggcc tgttcgcaga cgtcggagac cgcttgcagg
     4921 gcgacggggt gtccgtggag ggcgcagggt acgaagcggt gatgacggag gtgatactgg
     4981 agattgccgc gttggcggtc tcgatagctg gggtatgagt gtggatgcac atggcggatc
     5041 aggtggaagc gggcgtcgcc gtcggtcagg gacacggtgc gatggaagcg ggtgtggcga
     5101 tggacgtatt cgtcgaggcc gcggagccag tcaagggggt ggctctcaag ctcgcggatg
     5161 tcgggccagc gttcgttgtc gaggcgtttt ttgacgaact cgggctccat ggccggcgcc
     5221 tgctcccacc gcccgagccg catcacaccc gattcctcct cgcaggccag ccgacagagc
     5281 cgcaccaatg cgagcgttgc agcgccgtct tccagatatt ggacaacccg ttccacatcc
     5341 atccccgcca agccatgacg ggcggatgtt acccgtttct cgatgcccgg aagggctttc
     5401 ggcacccggt ccgggcgctt gggccacgcg cagcgcggct tgcgtgcagc gccttcccgc
     5461 ccccgcagcg gtataatcgc gcccttcctt tttcgccttc cggatcctgc cgtgcacaag
     5521 aaaatcctga tcctcgactt cggctcccag gtcacccagc tcatcgcccg tcgcgtgcgg
     5581 gagcagcagg tttactgcga gatccatccc tacgacgtgt cggaggactt catccggggc
     5641 ttcgcgccgg tggggatcat cctgtccggc gggccgaatt cggtgtacgc ggcggaggag
     5701 gtgaaggcgc ctcaggtagt gttcgagctg ggggtgccgg tgttggggat ctgctacggc
     5761 atgcagacca tggcggcgca gctgggcggc aaggtggaaa gctccggcaa gcgggagttc
     5821 ggctacgcgg agattcgcgc ccgtggccat tcggccctgt tccgcggact ggaagaccgc
     5881 agtaacgcgg acggccacgg cctgctggat gtgtggatga gccacggcga caaggtgacc
     5941 gagctgccgc cgggcttcaa ggtgatcgcc tccaacgatt ccacgccgct ggcggccatg
     6001 gccgacgaga accggggctt ctacgcggtg cagtttcacc cggaggtgac ccacacgctc
     6061 aagggccgcg agatgatcgc gcgcttcgtg cacgacatct gcggctgcac ccacgactgg
     6121 aacatgccgg actacgtgaa cgaggccatc gagaaggtgc gcgcccaggt gggcaccgag
     6181 gaggtgatcc tggggctgtc gggcggtgtg gattcgtcgg tggtggcggc gctgctgcac
     6241 cgggccatcg gcgaccagct cacctgcgtg ttcgtcgata acggcctgct gcgcctcaat
     6301 gaggcggaac aggtgatgga caccttcgcc cgcaacctgg gcgtgaaggt gatccacgtg
     6361 gatgcgtccg cgcagttcct cggccatctg gccggcgtga ccgatccgga agccaagcgc
     6421 aagatcatcg gccgcgagtt cgtggaggtc ttccaggccg aggcgaagaa gctgcccaac
     6481 gcccgctggc tggcccaggg cacgatctac ccggacgtga tcgaatccgc cagcgccaag
     6541 acgggcaagg cccacgccat caagagccac cacaacgtgg gcggcctgcc ggaaaccctc
     6601 aacctgaggc tgctggaacc cctgcgcgaa ctcttcaagg acgaggtgcg cgaactgggc
     6661 gtggccctcg gcctgcccca cgcaatggtg tatcgccacc ccttccccgg ccccggcctg
     6721 ggcgtgcgca tcctgggcga agtgaagaag gacttcgccg acctgctgcg ccgtgcggac
     6781 gcgatcttca tcgacgaact gcgcgccgcc gactggtacg acaagaccag ccaggccttc
     6841 gcggtgttcc tgccggtgaa gagcgtcggc gtgatgggcg acggccgcac ctatgaatac
     6901 gtggtggccc tgcgcgccgt ggaaacctcc gacttcatga ccgcccgctg ggccgagctg
     6961 ccccacagcc tgctcggcaa ggtgagcaac cgcatcatca acgaagtgcg cggcatcaac
     7021 cgggtggtgt acgacatcag cggcaagccg ccggcgacga tcgagtggga atgatctgac
     7081 atctcgcacg gtcaggcact ggccggtaag aaaacaaacc taagcccgcg taactgcggg
     7141 cttttttgtc acttgtatct ggcaacacca cgcacgacga tgcagcggta agcaactatt
     7201 ttccctggta cgctcaatgg tatcatttcc ttgcgctcgg aaattctggc ggataccatt
     7261 cattgatgtc ttaggaaatc atggtatcaa aatcgaagaa agctaggcag ggcgcggtgt
     7321 tacggcgatt ttcgtgattt ctaaaagcat ggtatctcag acgcctccga taccatagac
     7381 gactggctga acgaacagaa cgaaggggag aggcatgctt tccgatacca agctgcgcaa
     7441 tctcaagccg cagggcaaga tgtacaaagt ggccgaccgt gacggcctgt atgtggctgt
     7501 gacccaggcc ggggccatct ccttccgcta caactacacg ctgaacggca ggcaggagac
     7561 cgtcaccttc gggcagtacg gcatcggcgg catcacgctg gcggaagcac gggagcgtct
     7621 gggcgaggcc aagaagctgc tcgccgccgg caagtcgccg gcgcgagaga aagcgcgggc
     7681 caaggaccgg gagaaggacg ccaagacttt cgacgactgg gcgcagggct ggctgcgtgg
     7741 ctatgagatg gccgactcca cccgcgatat gcgccgggcg tgctacgaac gggaactgaa
     7801 aggtccgttt ggcaacaagt tgctgaccga aatcacccac gatgacctgc gccggctgac
     7861 cgacaagatc gtggagcggg gcgcgccggc cacggcggtt catgcccgtg aggttctgat
     7921 gcagatttac cgctgggcca atgagcgcgg acagcgtgtg gacaaccccg ccgacatggt
     7981 aaggccgagg gccattgcca agttccagcc gcgtgagcga gccctgtcgc cggatgaaat
     8041 cggcctgatg tatgagtatc tggagaaggt ggcggccggc cctcagttca aggtggcgtg
     8101 caagctcctg ctactgacga tggtgcgcaa gggggaattg accgatgcca agtggtcgga
     8161 gatcagtttc accgatgccc tctggacgat ccccaaggaa cgaatgaagc ggcgcactcc
     8221 acacctggta ttcctgtcca gacaggcgct ggacatcttt attgcgctca agacattcgc
     8281 cggcggatcc gaatgggtgc tgccgtcacg ctacgacgcc ggccagccga tgagtaatgc
     8341 gacactgaac cgagtgctgg agctggttta cacgcaggcg cagaaggacg gcaaggatct
     8401 gggcaagttc ggcccgcacg acttgcggcg cacggccagc accttgctgc acgaggccgg
     8461 gtacaacacg gactggattg aaaagtgtct cgcccacgag cagcgcggag tgcgggcggt
     8521 gtacaacaag gccgagtacc gcgagcagcg cacggccatg ttgcaggatt gggcagacat
     8581 gatcgacggc tggaccatca agaagcgatg accgtctgac cggggcgcac cgcctggccg
     8641 ccggaaactt tgcggccctc gatccacgcc ttgacctcgc ccaagtccca ggcaacattc
     8701 cggctggtga gggctatccg tttcgggaag tcaccccgct tctccatgtt gtaaatcgtt
     8761 cgatcggaga gtggaaccat gctcagaagt tgtctccggc tgatgaggac cagtccaggg
     8821 gtcttgagct cgttgtcttg gatggtgcgc atattccttc cggtacgtgc tgggtgttgc
     8881 caataatcta gcccttcccc atagcattct caccggtcaa aagcgcgcgc ttcggaaccg
     8941 tttcgaatca acctggcttc gcgcaggttc cggtcactcg tggccaccac ccgggtccaa
     9001 ttcggcgtga ttaggaaaac cgcctcgtct tgatcagtcg gtccggtgac agtgaccgag
     9061 cgagtggcag gacacggccc tttgccggtt gctcgatgcg ctcgcggaag cggacatacc
     9121 gtgctgttcg gcgcttcaca tccggctagg cagttggcaa gggccctctc ttccttcgac
     9181 gacatacttg cgaggatgta gcgtcctcag gaacaatatg ctggtgggct cattacggcg
     9241 gctgcccttg gaggaaggtt cattagctcg atacgatcca tagacctgcg cttcatagat
     9301 gacctcgtcg acttcgtccg aggtcccggc ttcgtgctgg acttctccga cgccagtttc
     9361 tcggactttt tcgcctccga gttgaaggtc gacatcaacg attcaaaata cgctgtgaat
     9421 ggtggttcca agggaaagcg cctgcgttgc ttcctgcaaa gctgcaatga cgccaccgcg
     9481 gttcgcaccc tcacggcgct ctgggaacac cgttccgagt gcctcgctcg caccggcggc
     9541 aaggatccag tcatcaatgc cgaggcccgt tatcaagcgc tcatcaaccg gctctcaggc
     9601 ggcgcgcctc cgcaaccggc ggcagccaat ccgaccgcca gcgcggttga tcgacagaat
     9661 ctcgcgaaga tcaaggcgga tcttctgcag gtcacatcgc tcagcccaca ggcgcgcggt
     9721 tatgcgttcg agggcttcct caagagccta ttcgacgcgt tcggacttgc agcccacgaa
     9781 cctttccgac ttcggggcga acaaatcgat ggtagcttcg agcttggaag cgacatctac
     9841 ctgctcgaag ccaaatggca cggccagccc atcggcgtcg gggagctgca taccttccat
     9901 ggcaagattg agcagaaagc agcgtggacg cggggcatct ttgtcagcaa cagcggcttc
     9961 actgatgacg gcctcgcggc cttcgggcgg ggcaagcgcg tgatctgcat ggacggcctc
    10021 gacctctacg agatactgga acgagagatc ccgctcaccc aggtccttga gcgcaaggtg
    10081 cgccgggccg ccgagaccgg gtcgcccttc gttcgcgtcc gcgatctctt tccttactga
    10141 ccgggaggaa agccgtggcg cagttcaacg attggtgcct ttcggtcgac gagccggtcg
    10201 gaaaccactt ccgccgggtg atgaccgggc aggccggcaa ccttccgacg gggatccaag
    10261 cgacagcggc catcgtcccg gggcactatg cgtcggagga acaggtggtg cgcgcgttcg
    10321 cccgtctcgg caagccggct gcggccgcgc taatcgaggg caaactcccg acaaccaagg
    10381 cgatccgctc cggcgatctc ggcgaaatct acgcgacgga atggatcgat gcgcatagcg
    10441 gcggttatcg cgctcccatc aagcgtctgc gctggaaaga ccatcgcaac atggcgatgc
    10501 gcggcgaaga cgtcatcggc atccttcaag acgcccaaac ccagcggctc catttcctaa
    10561 agaccgaagc gaagagccgc gcgacgctca ccgctcaagt cgttaccgaa gcccgggcct
    10621 gcctggataa ggatggcgga ttgccgtccg cccacgcgct ctcctttatt tcggcacgcc
    10681 tgctcgagtt gaacaatttg ccgctcgccg acgccatcga tgatgcactg ctcaagcacg
    10741 acatcccggt acagaacgtt cggcatctgc tcttcacatt ttccggcaat gcgccggacg
    10801 ccctactaac cgcctctctc caggcctacc cgggaccgat aaatcagtgg gggatcggcc
    10861 tgcacgtaga gggccacgcc gctttcgtcg gcgcggtata tgatcaagtg atcgccaatg
    10921 ccaacaacgc ctgaggcaat cgcggccgat atcgcagaag cggcgacagc cggctttcgc
    10981 ggccgcctca ttgcgcgtgg ccaggcacga gcaatcatct ggcgcaacgg tgagcttccg
    11041 ccggacgcgc cggcctttgc gcagcagctc agctacgacc tgcactccta tggctacgcc
    11101 cttctcgggc tcggccttcg cctgcgtgag ctcggcggcg acaccgccca ggcacgtact
    11161 gccttcgagc aggccgccac cgcccttgag gccgtcatcg ccaagggcaa tcgccaggag
    11221 gccgatcgtg acttccactt catcatggca gcggcaagct atcatctcgc ccatctgtcg
    11281 gcgcgcgcat actcactcct cgcgatcgtc gaggccgacg aaaatttttc gcccatcgaa
    11341 cgggtacttg cccagttgat gcggcggaat ttccgtgctc ttcgagtcag cgtgctcgac
    11401 taccgcgcct ccggcgaagg cagcgatgcg caaatcgccg ccgccatcca ggccaatctc
    11461 gatcaagccg aagccgcggc tgagccagca gacggcagca atgacttcct cttcgacggc
    11521 ctcgatatcg cgctgaccga cgccttcatg gcggccatgt ccctgtttct cctcgcgctg
    11581 gaacgcggcg agcagccgct cctcgaccaa gcgttggagc ggcttcggac aagcctcgca
    11641 atctgcagcg agttgaacat gctgccgcaa tggtgggcgc atcgcatcgc gatccacctc
    11701 ttgtcggacc tttggtcgag caccttccac gagaacgtgc cgctccagcc cgccggcggc
    11761 gaagcggtcg actggccgcg gcttcgcgag ctgttcatcg cgctgctgca acagcgcccg
    11821 aaagccgaag tggacctgtg gccatcccag atcgaagcgg cgtcccgcgc ggtcgaccaa
    11881 tccgacgatc tggtggtctc gctacccacc agcgccggta agacacgcat cgctgaactg
    11941 tgcatcctgc gctgcctggc cggcggcaag cgcgtcgtct tcgtaacgcc gctgcgcgcg
    12001 ctgtcggcgc agacggaaac gaccctgcaa cggacctttg gcccgctcgg caagacgatc
    12061 tcggcgctgt acggtagcat aggggtgagc ggtttcgatg aggacgcgat ccgggaacgc
    12121 gacatcgtgg tcgcgacgcc ggagaagctc gacttcgcgc tccgcaacga ctcttccctc
    12181 ctcgatgacg tcggcctgct cgtcttcgac gaaggtcaca tgatcggcct caacgagcgc
    12241 gaggttcgct acgaggtgca aatccagcgg ctgcttcgtc gcgcggacgc gcacgagcgt
    12301 cggatcgtgt gcctctcggc gatcttaccc gatggcgatc agcttgatga cttcgccgga
    12361 tggctgcggc gagatcaccc cggcgccgtg atcaggcacg actggcggcc gacccggcta
    12421 tggttcgggg aggtggtttg gtcctcgcca aacgcgcgcc tgaatctgcg tatcggcgac
    12481 gagcgccctt gggtccagcg gtttctcacg ggagcagtgc cgccgaattg ggtgccgcca
    12541 aaacgtcgtc gaaccaagct cttcccatgc gatcaacgcg agctctgcct cgcgacggcc
    12601 tggcggctcg tcgaggatgg gcagaccgtc ctcatcttct gcccggaacg acgcagcgtc
    12661 gagccgttcg cagacgtaat cgtcgatctc catgagcggg gcgcgcttcg ctcgctgctt
    12721 gaatccgacc ccgcggtcct caacaccgcg atcgcgctcg gcgaggaatg gctcggaccg
    12781 gacagcgcca tcatcaaatg cctccgcctc ggcgtcgcgt tacaccacgg ggcgttgccg
    12841 accgcctatc gcaaggaggt tgagcgcctg ctgcgcgaca acatcctcaa ggtcaccatc
    12901 tcgtcgccga ccctggcgca gggcctcaac ctgtcggcca cggccgtcgt catgcactcc
    12961 ctccaccgct tcggcgaacg gatcgagatc tcagagttca agaacgtcat cgggcgagcc
    13021 ggacgggcct atgtcgacgt ggaaggcacc gtgctttttc ccatgttcga cggcatcgcg
    13081 aagaagcgga gtaactggga agccctcatc aacgatctcg gcgcgcgcga tatggagagt
    13141 gggctggtgc ggttggtggc ggcgctgctg tcacgcatgg gcgcccgcat cggcggagat
    13201 ctggaccaac tcgtggagta tgtcgtcaac aacgccgccg cgtggacctt ccccgaaatc
    13261 gcgaacgaga agcccgagga tcgcgagcgg gctcttgccg attgggaacg gcatatcgcg
    13321 acgctcgaca ctgcgatcct cagcctcatc ggcgaaaacg acatcccgga cgatgggatt
    13381 gaggccgcgc tggatgacat cctccagtcc tctctctggc accgccgcct actacgccag
    13441 aatgagcagg tgcagcaagt cctcaaggcg ggcctcgttt cgcgcagccg gcacatctgg
    13501 aatcagtcga cggcggcgcg gcgccggggc tacttcctag ctggtgtcgg tttaacgacc
    13561 gggcatgcgc tggacgccat cgcagcggat gccaatcttc tcttggttca ggccaatgcc
    13621 ggcatcctcg agaatgactc tgaggcgacg atcactgcca tcatgagcat cgccgagcgc
    13681 gttttcgcct tctatccctt cacgccagat cccatgccga ccaactggcg ggacattctc
    13741 cgagcttggc tcctcggcca gcctctcgcc accatcgcgg caggtcacga gtcggatacg
    13801 ctccagttta ttgagggggg actcgtctat cgcctaccct gggcgatgga ggccatccgt
    13861 gttcgtgcgg ccgccaacgg cgacaccatc ggcgacttcc tgctcgacga ccacgagctt
    13921 ggcttggcgg tccccgccgt cgaaaccggg acgctgaacc gctcggcctc gatccttatc
    13981 caggcagggt tcaactctag gcttgctgcc atcaaggccg tcaccgatac tggggcgacc
    14041 atccggacgg gccaggagct tcggcaatgg ctcaattcgc aagccgtcgc cgcgttgagc
    14101 gctcagcccg attggccgac cgccgaaacg aaggtgatgt ggacggaatt cgtgcaaagc
    14161 ttcatcccgc gcgagaacaa gacctgggcc gatcgccgat accgggcaaa tgtggcctgg
    14221 ctcggcgtgc cgccgccgcc ggacacgccg gtgcagattc acgaatgggc cggtcagcca
    14281 cgcgtgctct cggtcgacgg tgcgccactg ggcacggtgc aggctgcact aaatcacgcg
    14341 cgagcggggc tggtgcgcgc tcaagtggct caggacctca gcaaaatcga catcacttat
    14401 ctgggccctg acgacctgtc tgttgcctga tgcgctctaa gagcacggga gcctctggag
    14461 gaacttcacg agcttcgcca tgacagtggc gcagccctaa tccgacttcc ggcatcgcaa
    14521 cgccactggc cgcacttggc ctgctacagt cgatgattat gatcatcgat ctgggtgagc
    14581 ccgtcgtgtg actacctgat caaccggatc ggaattcacg gccagagccg ggacaagcga
    14641 gaatggtgca ggcgccaggg gtggtgacgg cgagctcgtg gcggggatca gatccaacta
    14701 tacggctggt gccgggacag ccgcagccac ttcgtcgcca gcgctggcag cggcatcagg
    14761 aacatctttg gcgtcgcctt gcccgtcctt cctgcgggct gcgatcatcg cgcgtccggc
    14821 gattgaccgt atgcctcccg ccagccggag cagtcggcgc ttccattcgt agatgctgcg
    14881 cgaataggcg ctgtccgcga tgacgccgga cagccacagc gtgtcgagct tggcgatcag
    14941 gccgtcgaat tcccggatca ggcccacgta gcgcacggcg cggggagagg tgattttcgc
    15001 ctcaacttcc ttggggtggg agtaatcgat gcccttgaac tcgatcccgt tcgcctcggc
    15061 catcttgtcc agacgggacg cctcgttgcg catgtcctcg aacatcttgt tcaggcgctc
    15121 gtcgacgagt ccctcgactt cgtgcgcttg ttcgtcggtg ccgatgatcc gcagcacgac
    15181 cgagagggag aagatggcgt tggcgcacag ctcaaagccc cggtcgaatg cctgctgcgc
    15241 atgcaatgaa tggagcttta ccttctgggt gaagtaggga tggctcttgg cgttgtcccg
    15301 cgcggcgcct ggcatggtaa acctcccgga gagatggaca gggaaccatg ctacgcaagg
    15361 gagccaggga aaccacggct gaaaagcgca ccataacgca ccgtttggca gttgcagttg
    15421 cggcagggcc ggatagaatt tccatcatca tacgaagtgc ataacgcctt cgatgcagcg
    15481 gtagctggcc gctcgaaaca accagcatcc aatccgccga ccgtgccatg cgcggcggcg
    15541 agtcaaaggg gccacggcgc ccaaccctgc cacgggaaat agtggcaacg gattgggcgt
    15601 gtcgtcacac ctccgccaca cggcgttgag gcagcgcgcc ccgtgaacct gcgacaaagg
    15661 atcacctaat tgagatgcca ccccggaagg gcgtcgtctc ccgcccgcgt tccgcgggcc
    15721 ggtttctcaa ccctgcgggg gccatcatgc gcccggcggg gtcgtggtgc ctccgcgact
    15781 ttcttcaagg aggcacgatg gaaaaggttc tgctcgttat ggccttgctg ggctcggcgc
    15841 atctcgcgtt tcggggagtg ggcaaagtcg ggaagctggt gatcgcgttc gctgcaccga
    15901 aatccggcgc cgtctccagt gtcgttcgga tgccggaggt gaggccgatg gctgtccgca
    15961 tggacggcga ggaagactgg tcgaagtacg acatcccggc gttcatccgg cgtggcattc
    16021 cgatgccgac gctggagccg atgccggcga aggagaccaa gacgcgcaag cgtcgcagca
    16081 aggtgaaagc ggaaggtgcc gcaacaccgg cgtcatccga caccgcgacc tttgaggtcg
    16141 tggcgtagtc aatcaggaac cagaccgggg aaatcgttcg tcgacggaat attccggtcg
    16201 gccggaatta gccgttcacg gcgaaccaag gtgcaagtcc ggttccacct tcttttcaac
    16261 cctgcggggg cgatcacgct cccgcaaggg gccgtggtgc ctccgctttt cttttgtgga
    16321 ggcatcatgg cagccgtcat tatcgcttgc tgcatttccg gttgggtcat cagccgtccg
    16381 atcgtccggg cgctgggtct gtaaccagaa gtgcaactcg cctgtgggaa tcccgcaggc
    16441 ccgaggcctt ttcacggaga ggcttcgggc ctgcggggtt gccacgggcg cgtatcaagc
    16501 ctggcaactc ctgcgggatc gatcaaccaa tcttgcaagg agcgtcgcca tgatattcgt
    16561 gatcctcttg atgtgcgtct tctccgggat cgtcatcagc cgcccgatcg ttcgcgcgat
    16621 tgggctgcgt tgatacccgg agaccgccgc catgtcgaat cacctgctga agcgcctata
    16681 ccgctcgatc ccctttgcct tggcgtgcga actgcatcgg cgccttcagg ccggctgcac
    16741 cgcgcctgat acgcgaaaga tgttgcgcca cgccttgagg aaatccggga tcggccggtg
    16801 tgcggcttga acacgcgccc cgcagaccgc ggggctttcc ccagcgcatt tttcagagtg
    16861 cgctggggaa agcggaattg ctccccactg gctctgccag ggggcatgaa ggtagctggc
    16921 cttccgaaac aaacagcatc gagctgctga actgggccac cggatcagca ttcatcaacc
    16981 ccggccgggg catttgcccc ggtttggggt aggtgcttcc ggcgaaagga ggcactatga
    17041 agatggaaaa catcagcctg ctgtccatgc cggctgacca ctaccacttg catcccgccg
    17101 tgggccactc cgggctggtg cgcatcatgc gctcgcctgc ccattaccgg gagtacgcca
    17161 ccaatccgcc tgagccgacg ccggcgatgc aattgggaac ggccttccac acggccctgc
    17221 tcgaaccgga tcggttcggg gagaccttcg tggtggcgac caagttcgat cgtcgtacca
    17281 aagacggcaa ggccgcggcc gaagcctggg aagcggacaa tgccggcaag accgcattga
    17341 ccgccgagca gttggaggcg attcggtcga tggcgtacct cgtgcgctgt catgccggcg
    17401 ccgacaggct gctctcccat ggcctggccg agatgtccgg cttctggatc gatccggaga
    17461 gcggcgtcga gtgcaagtgc cgcccggatt tcctggccat ggacggggac cgaatcaccg
    17521 gcatcgtgga cgtgaagacg tgttgcgacg ccggcaccga cggcttcgcc cgcgccatcg
    17581 caacgcttgg ctacgacgta caggcggcgt tctaccagga cgggttcaag accctgacgg
    17641 gcaggacgat tccgttctac ttcatcgccg tcgagaagga cgcgccctac gcggtggcgg
    17701 cctacaaggc gagtgacgag gtcatcgaag tcgggcgcgc caagtaccgc ggcggccttc
    17761 agctcctgaa gtggtgccga gagcgtgaca tctggccggg gtatcagccg agcggggaga
    17821 tcgagacgat caacctgccg cgctgggcgg cccacttcga tctggaggcc tgactacgtc
    17881 gactcggaaa tcgaggcagc cgaagtgatg tgcaaccggc acaaggccgc atcgaaggcg
    17941 tgacgggttc cgaacccgag acgccacgat cggcgcaaca gcagcagaag caatagcaac
    18001 ccctggcggg aacgtcatac ctcgccaagg ggcatggtgt gaccgccgtt tctttgtcat
    18061 ttttcgatgg aggtcacgac atggcagcac tgcaagctct caagaacatc aaacgcgaca
    18121 cgcccgccga agggcagccg caaggctttc cggccatgct ggagaagttc aagggcgaga
    18181 tcgcccgggc attgccccgg cacatcaacc ccgaccggat ggcccgcatc gccttgacgg
    18241 cgtttcgcat gacgccgaag ttggccgagt gcgatccgcg gtcggtcttc gcggcggtca
    18301 tccagtcctc gcagttgggg ctggaggtgg ggctgatggg agaggcgcac ctggtcccgt
    18361 tcaaggggca gtgccagctc attcccggct acacgggtct catgaagctg gcgcgccagt
    18421 ccgggctggt ccaggacatc tatgcgcacg aggtccgcgt gaacgacaag ttcctgctga
    18481 aactcggcat ggagcgttcc ctggagcacg aaccgctgac cgcccctggt ggcttccctg
    18541 cgtccgatga ggagcgcggc gaagtagccg gcttttacgc cgtcgcggtc ttcaaggatg
    18601 gctcccgtac cttcgtggcg atgagccgca aggaggtcga gaagatacgt gatggcagcc
    18661 gcggctatca gagcgccaag caatacaaga aggaatccgt ctgggattcc gacttcgttg
    18721 cgatgggcct gaaaacggtc atccgtcgcc tgagcaagtt cctgccgaag tcgccggaac
    18781 tggccacggc cctggcgatg gacgcggcga acgagcaggg aagggcgcag aacctggatc
    18841 tcgacgacgt catcgagggc aactatgccc ctgtcgccga cgagacgggc gatgtagacg
    18901 ccgacggcaa ggaggatgcc aagccggaac gggcgcccca ggaaaccaag gccgaggccg
    18961 cgggcgcgca agcaaccaag gtcgaaagtc atggtgccac ggcttccgtg atcgagcccg
    19021 gcaaggacgg caagggcaat ccgaagctcg acgcagccat caaagccatg gaaaaggcga
    19081 aaacggtcga tgccctggac gaagcctaca tccgagccga agccgagttc gagggagccg
    19141 atctcgaatc cctgatgcgt gcttaccagc gcatcaaggc acggatcgcc accctgatct
    19201 agaagcatct ccccgcgctc gcgcgcgggg ttttcccgag tgcattttcc gaagcgcact
    19261 cgggaaagcg gaattgctcc ccactggcgt tgccagggga catgaaggta gctggccttc
    19321 cgaaacaaac agcataaagg cgaca
//
LOCUS       DAOQPA010000067        19221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1431_length_19221_cov_18.225295, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000067 DAOQPA010000000
VERSION     DAOQPA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19221
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_19221_cov_18.225295"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /gene="fadH"
                     /locus_tag="PKX01_10455"
     CDS             complement(<1..1177)
                     /gene="fadH"
                     /locus_tag="PKX01_10455"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990030.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HMZ76591.1"
                     /translation="MNAIPASAYPRLLAPLDLGFLTLRNRVLMGSMHTGLEEGEDPHA
                     RLAAFYAARARGGVGLIVTGGYGPNPDGAIHAGGSVFDSEDEARRHRVITDAVHAEGG
                     RICLQLLHTGRYAYSRSPVAPSAVKAPINPATPRALDEDEILRTIADYARAATLARAA
                     GYDGVEVMGSEGYLINQFLAPGTNLRDDRWGGSFENRCRFGLEVLRAVRAAAGHDFIV
                     IFRLSLLDLVEGGSTWDEVLAFARAVEQTGVNFINTGIGWHEARIPTIMSSVPHGAFT
                     ALTARLRGVVNVPLITSNRLNDPATAEAVLARGDADMVSMARPFLADADFVAKAAAGR
                     ADTINTCIGCNQACLDHIFAGKTSSCLVNPAACREWEGPAAPARTPRRVAVVGAGPAG
                     "
     gene            complement(1436..1933)
                     /locus_tag="PKX01_10460"
     CDS             complement(1436..1933)
                     /locus_tag="PKX01_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HMZ76592.1"
                     /translation="MRIPSAPSLLFALLAGSLALPALADMPCGPMGGPMAPPGASRDN
                     RMERHTLHHAHLHEALKLTPEQEPAWQALMASEPTPMPAPTEDWRSLPTPERAERMLT
                     RMKAHEAQMSAHVDALKTFYATLTPEQKKTFDAAHAVGPRGMKGRMGPGMMGGGTGGR
                     MSDRP"
     gene            complement(2126..2989)
                     /locus_tag="PKX01_10465"
     CDS             complement(2126..2989)
                     /locus_tag="PKX01_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMZ76593.1"
                     /translation="MNTRTHRSPLRASLALCALGLLTGTARADVRPDPGDYTGLPAGT
                     NLLLGYAQFPRADKVYSHGNQTVSNLDLSINAGIFRYVHFMKLGDYIIDPQIIVPFGT
                     QKIGLSDSKTSGLGDVIFGGTLWTLADLTRGEHLGWSVFFTAPTGSDRNQGFALSNNR
                     WATDLQVGYIRKLSDTWTLDLIGETEFYQDQRDTKARKDPLYQGHAHLRYFLSGATYL
                     AGTYRHAWGARESLNGADIAGRKNNGTAMLTWASFVDKQWQVQLQYTQDVKVEDGPKV
                     NGVQARVLYVY"
     gene            3463..5409
                     /locus_tag="PKX01_10470"
     CDS             3463..5409
                     /locus_tag="PKX01_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMZ76594.1"
                     /translation="MGEMQRAFDARMLRARRAFFEDGTRPSGVIPDAVLRSWERSRQQ
                     GLGVRDRRIFMPVSREEVGRLQAESSELMACALPEMERLQAALGRGGWGIVCTDAQAR
                     VLKAVYGRSTAFREMDRIFEIGRNLSESAIGTNGPGCALIEGRPLTVRAAEHFLDETR
                     PFTCASVPLFGPDGRIVGVLDATRYCDGAAPTVVEPLAIAARAVENRLLGRLGAALKI
                     AFHCREDMLGTPMEGVLVFDGDGVLRGANQAARQLLSLTAGDDGGVRFDSVFDCPPET
                     FGRCLGGRVERTVLWGGEGMRFELRVEAAQPRPVLARPASVPSRAGSGWASAPSAACT
                     RDDGEDAAVARALHVAQRAFERDIPVLVLGETGTGKEVLARRLHGDSARADGPFVAIN
                     CSSLPAGLIESEFFGYEDGAFTGGRRGGAPGKFELAQGGTLFLDEIGDMPLELQGRLL
                     RVLQERNFSRLGGARLIHFDARIVAATHRKLDEAVAAGAFREDLYYRIKGVRVRLPAL
                     RERADRAALIDTLLRRECGGRRPPPLSAEVRAVLLGYRWPGNLRQLGHVLRLALTLAE
                     DDGEIGLQHLPEELLEAGSPTGGGLAPDLREQCLREVELDAVRAAMARCGGNVSAAAR
                     SLGVARATLYRKLRMLGMVGEGEA"
     gene            5909..6559
                     /locus_tag="PKX01_10475"
     CDS             5909..6559
                     /locus_tag="PKX01_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ76595.1"
                     /translation="MSESSPPRHQRRKEARPAELAAAALSLFVEKGFAATRLDEVAAR
                     AGVSKGTLYLYFDSKEALFRAVIEGSVVPLVEQSEARIEALKHDPERLLRETLVNWWF
                     NFGATELGGICKLMISEAGNFPEVAAYYNEAVIVRWMNLLGRVLELGMEQGVFRRLNV
                     DILRQQVFFPLLMQSVWKNSLAGCCAPVALPDSYFDTYFDLVFRGLLAAPPLEPSR"
     gene            6556..7647
                     /locus_tag="PKX01_10480"
     CDS             6556..7647
                     /locus_tag="PKX01_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ76596.1"
                     /translation="MMRPLVLAVAVATVLAACSKPEPVAEAPRPVVAQKLTPVAVASG
                     ALYTGEVRARYESDLAFRITGKIVERKVDVGAVVKPGQVLARLDPADAGLNAEAARAQ
                     LAAAQTDLAFAKAEVERYRDLVAKHFVSRSVLDAKEATFNAAAARVAQARAQASVAGN
                     QAGYTTLVADQAGVITAILAEAGQVVKDSAVVMRLARDGEREVLIAVPESRVAALRKA
                     GQVEIRLWAQPERALEGRVREIAPSADAATRTYAVKVTILSNDPTVQLGMTANVILPD
                     AAAGALLVPSTAVFEHAGGSAVWVLQADGELVKPVLRPVTVKQYREDGVILSGGVQAG
                     ETVAVAGVHKIVAGQPLRPVAPARPGAGA"
     gene            7644..10775
                     /locus_tag="PKX01_10485"
     CDS             7644..10775
                     /locus_tag="PKX01_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ76597.1"
                     /translation="MKRFNLSEWALRHQALVRYLIVALALVGVMAYQRLGQSEDPPFT
                     FKVMVVRTEWPGASAREIEEQVTDRIEKKLQEVPFVDYLRSYSRPGESVVLFVAKDSA
                     PASAIPDVFYQVRKKIGDIKGMLPTGIRGPYFNDEFGDVFGNIYALTGDGFDYAALKS
                     ASETIRAELLRVPNVAKVDLFGEQEQRIYVELSNTKLATLGLDVQTVTAALAQQNTVA
                     PGGYFETRDDRIYLRPSGAYDTVEAIRETRIRAGGREFRIGDIATVARGFVDPPAPRF
                     RYRGHDGLGLGVSMAKGGDIIALGQNLKEAVARIQAQLPVGMELAEVASQPEAVQRSI
                     SEFVHSLAEAVIIVLVVCFFSLGLRTGLVVALSIPLVLAVTFFGMRLFDVGLHKISLG
                     ALILALGLLVDDAIIAVEMMLVKMEQGWERARAAAYAYTSTAGPMLSGTLVTVAGFLP
                     IATAQSSTGEYTRSIFQVNAIALLASWLAAVIVVPYLGYVLLPDPRKAAGPGWLERRV
                     PAFARLKARLGIGGHTHHDGDEDAVFQTPFYNRFRALVDRCVARRWWVIGATLAIFVL
                     ALVGFGQVQQQFFPNSTRLEILVDLRLPEGSSLRATQAEAARLEAILDREPGIANYVT
                     YVGTGSPRFYLPLDQQLPAANFAQLVILTDSIPAREALRQRLIELFRTDFPGLRANVT
                     RLENGPPVGFPVQFRVSGPDIPTVRRFAAEVAEVMRANPNLSDVQLDWDEQSKVLRVE
                     VDQHKARLLGLSTQDVANFLNTSLRGLTATSYREGIESIDVVLRGDAFERDKLSLVSE
                     LSIPTRAGKSVPLGQVATVSYAWEQGLIWRRDRLRSITARGQIYDSRLQAPTVTAQVE
                     PLLAPIKARLPAGYRIDTGGAVEESAKGQSSVNAGFPLFFAVVFTVLMVQLNSFSRTT
                     MVVLTAPLGLIGVVLFLLLFNQPFGFVAMLGTIALFGMIMRNSVILVDQIEQDVEKGR
                     SVWNAIVESAVRRLRPIVLTAAAAVLAMIPLTRSAFFGPMAVAIMGGLIVATALTLLF
                     VPALYAAWYRVRR"
     gene            10879..11532
                     /locus_tag="PKX01_10490"
     CDS             10879..11532
                     /locus_tag="PKX01_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HMZ76598.1"
                     /translation="MNFEQARFNMVEQQIRPWEVLDFSVLDLLMSIRREEFVPEAYKS
                     VALSEAPIPLGHGASMLVPVIEGKVLQAIQVKGSDKVLEVGAGSGYFAALLAAKAEWV
                     RTVEIEPALVNMAHDNLKRYGVENVIVEEGDAVSGWAGNAPYDLIVVSGAVPHVPESL
                     LAQVKVGGRLFAFVGGPTLQVATLIQVVGEGQYRTEKVFETQVPMMRNAPRKNQFQF"
     gene            11545..11868
                     /locus_tag="PKX01_10495"
     CDS             11545..11868
                     /locus_tag="PKX01_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ76599.1"
                     /translation="MQQILPAQLDEWLRDASRPQPVLLDVREPHEFQYCALAGSLSMP
                     MASVPARQQELDPDAETVVICHHGMRSMQVALFLERQGFGKVINLTGGVALWAQQVDP
                     SMPQY"
     gene            11887..13206
                     /locus_tag="PKX01_10500"
     CDS             11887..13206
                     /locus_tag="PKX01_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HMZ76600.1"
                     /translation="MSLSKVRFAVLAWAGVMASGAQAVDLEQAYRDAVTYDATLSGAR
                     ASLAAGREKLPQGRAGLLPTISLSGNTNYNTAVFNNLDPHFTSKYNSHSWIAQLNQPL
                     FRWQNWVQFKQGELNVGLAEAQFKLAEQDLVLRVAQAYFDVLAAQDTLVAASALRDAN
                     AQQLALAKKSFEVGTVTITDVHEAQSRYDLSSAQTIAAESDLEVRRNALAVLTGKTPD
                     ALKTLRKGVALSSPEPADMPSWVSASEATALQVQAAQLGVEIAAREIERNRAGHAPTV
                     DLVASYGNSVNPVASVTPTTNYDSRAIGLQVAIPLYQGGGVNSRVREAIALRDKAAAD
                     LDNARRTAAQAARQAYLGVTSGLAQVRALEAAQVSSRSALDANKLGYEVGVRINIDVL
                     NAQSQVFDTEQKLSKARYDTLMAQLKLKAAAGTLGDDDVRALNALLD"
     gene            13342..14814
                     /gene="ubiD"
                     /locus_tag="PKX01_10505"
     CDS             13342..14814
                     /gene="ubiD"
                     /locus_tag="PKX01_10505"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HMZ76601.1"
                     /translation="MRYTDLRDFIAQLERLGELKRVRVPVDPHLEMTEICDRVLRAGG
                     PAILFEQPTGHSVPVLANLFGTPQRVALGMGESGADWQSALREVGKLLAFLKEPEPPR
                     GLKDAWDKLPVFRQVLNMSPKTVSSGPCQEVVREGRDVDLASLPIQHCWPGDVAPLIT
                     WGLVVTRGPHKPRQNLGIYRQQVLGPNKVIMRWLAHRGGALDFRDHQQAHPGEPFPVA
                     VVLGCDPATILGAVTPVPDSLSEYQFAGLLRGAKTELVKCLGSDLQVPASAEIVLEGV
                     IHPGETALEGPYGDHTGYYNEQAEFPVFTIERITHRRDPIYHSTYTGKPPDEPAMLGL
                     ALNEVFVPLLQKQFPEIVDFYLPPEGCSYRLAVVSIRKQYPGHAKRVMFGIWSFLRQF
                     MYTKFIIVVDEDVNIRDWKEVIWALTTRMDATRDTTLVDHTPIDYLDFASPVAGLGSK
                     MGLDATNKWPGETQREWGRAIVMDAAVKARVDALWAQLGL"
     gene            14871..16463
                     /locus_tag="PKX01_10510"
     CDS             14871..16463
                     /locus_tag="PKX01_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ76602.1"
                     /translation="MLCRMLARALLSAGVILMPAFASADLVGAGAVQFETDRLAEGQA
                     ALRSRQLERAERAFSEALRANAGSTDALIGLAQTAQLQGRPDKARDWMAKAVAAAPGS
                     PPILQAQSKLLVEQGMGALAEEHYRRAIAANPGNPQFSQDLASLYLEQLKRPAAAVTV
                     LRDLLRRQPELASAYLGLGLALAADGKLDEAGRTLDEAVQRDPVNPFAYHAQGLLALK
                     QGRADAALTAFDKALALRPEFVGALLGRGDALLALGKADGALATYRRAASLAPGSAVP
                     IAKRAQALERLKRPDEAEAAYREALKLEPTHTVVLNNLAFLLASRKQRLDEALGLAQR
                     ATTLDPARADHFDTLGVVLLARGDQAGARKAFERAVAVDPAHAGARQHLARVGAPAEA
                     PAAAGPSAPAASPREIPKEAARSSTDDAARALSTRLEAWRQAWESKDAGRYLSFYGKD
                     FAPPDGRDRGTWEAERRVRLDKKGEIHVQIVAPELRVEGDTAAVSFEQRYRAGALVDT
                     GRKRMAWHREGGEWMIRREGMQ"
     gene            complement(16476..17657)
                     /locus_tag="PKX01_10515"
     CDS             complement(16476..17657)
                     /locus_tag="PKX01_10515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ76603.1"
                     /translation="MFIRIDQLIASPRRLLSAIVLFVVLDLAVLLINVWIAAQVAEDA
                     VAINLAGRQRMLSQEITKALLLAVHVPSADARRDAEQELRTAYRLFERTLRAFHDGGD
                     TAGGTGPSVHLRPIDPGAGLDAVERVATLMAPLDARLPEFLAGKPLADSELGEATHYM
                     VQHNRDILAEMNRLTSALEQASVQRTRTLRSIQIGAFVLALGNFTVIVVGLVRRYRNL
                     TQESQRWRRLAEFDPMTGLASRTAYREARDSTHDAAVRWQRSATLLLIDLDGFKPIND
                     TFGHAVGDLLLAAVSERLRQVARNTDLVGRLGGDEFALICLDISAENDIGPLCERLLA
                     EIAALTTEDGRRYGVTGSIGAAVIPGPYPSADAWEAAADAAMYAAKRRGGNAWRLASA
                     V"
     gene            complement(17799..18983)
                     /locus_tag="PKX01_10520"
     CDS             complement(17799..18983)
                     /locus_tag="PKX01_10520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ76604.1"
                     /translation="MQVKKLTDLDVKGKKVFIRADLNVPQDDAGNITEDTRIRASIPS
                     IRYAVEGGAAVMVTSHLGRPTEGELKAEDTLAPVAVRLGQLLGKPVRLIQNWVDGGFD
                     VAPGEVVLLENCRCNKGEKKDNEELARKMAALCDVYVNDAFGTAHRAEATTHGIARFA
                     PTACAGILMGAEIDALGKALHSPKRPLVAIVGGSKVSSKLTILKSLADKVDQLIVGGG
                     IANTFLLASGKRIGESLAEPELVKEAQVIIDLMKARGAEVPLPEDVVVADEVSAMARA
                     NKVPVDEVGPHDRILDIGPKAAAQLSQIIANAGTIVWNGPVGVFELPQFAGGTKMMAS
                     AIAHSEAFSIAGGGDTLAAIAKFHIADDVGYISTGGGAFLEFLEGKTLPAIAALEERF
                     KD"
     gene            complement(19051..>19221)
                     /locus_tag="PKX01_10525"
     CDS             complement(19051..>19221)
                     /locus_tag="PKX01_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HMZ76605.1"
                     /translation="SSDFNHTTVSSTFDATQTRVIKGEDGSVLVKVLAWYDNEWGYSC
                     RMLDAARAWMNV"
BASE COUNT         2843 a         6217 c         7012 g         3149 t
ORIGIN      
        1 gaccggccgg gccggcgccc accacggcta cccggcgcgg tgtccgggcc ggcgccgccg
       61 ggccttccca ctcccggcag gcggccggat tgaccaggca ggacgaggtc ttgccggcaa
      121 agatgtgatc caggcaggcc tggttgcaac cgatgcaggt gttgatggtg tcggcgcggc
      181 cggcggcggc cttggcgacg aagtccgcat cggccaggaa ggggcgggcc atggacacca
      241 tgtccgcgtc gccgcgggcc agtacggcct cggcggtggc cgggtcgttg aggcggttgc
      301 tggtgatcag gggtacgttc accacacccc gcaggcgtgc ggtgagggcg gtgaaggcgc
      361 cgtggggcac gctgctcatg atggtgggga tgcgcgcctc gtgccagccg atgccggtgt
      421 tgatgaagtt gacgccggtc tgctcgacgg cgcgggcgaa ggccagcact tcgtcccagg
      481 tgctgccacc ttccaccagg tccagcaggg acaggcggaa gatgacgatg aagtcgtgtc
      541 cggcggcagc gcgcacggcg cgcaggacct ccagcccgaa gcggcagcgg ttctcgaaac
      601 tgccgcccca gcggtcgtcc cgcagattgg tgccgggggc gaggaactgg ttgatcaggt
      661 agccctcgga gcccatcacc tcgaccccgt cgtagccggc ggcgcgggcc agggtggcgg
      721 cgcgggcgta gtcggcgatg gtgcgcagga tctcgtcctc gtcgagggcg cggggcgtgg
      781 ccgggttgat gggggccttg acggccgacg gggccaccgg cgaacggctg taggcgtaac
      841 ggccggtgtg gagcagttgc aagcagatcc gcccgccttc ggcatggacg gcgtcggtga
      901 tgacgcggtg gcggcgggcc tcgtcctcgc tgtcgaacac cgagccgccg gcgtggatcg
      961 cgccgtccgg gttggggccg tagccgccgg tgacgatgag gccgacgcca ccccgcgcgc
     1021 gggcggcgta gaaggcggcg aggcgtgcgt ggggatcttc gccttcctcc aggccggtgt
     1081 gcatggagcc catcagcacc cggttgcgca gggtgaggaa gccgaggtcg aggggcgcga
     1141 gcaggcgcgg gtaggcagac gcagggattg cgttcatgaa gggctgtctc ctggaattgt
     1201 gcttggtgtt gtgccgcgca ttgcaggggc gggcgcggga aaagtcaagt tgccgtgacg
     1261 gcagtcgggg cagggccagg agcgcggtgc gtgcgggcgc gggcagtacg ttcgacatcg
     1321 cggcgggaag cgccacgcag ccgggatctg gcggacggag acgttggggg ccggtcggcg
     1381 tgtggggagc gacctgccgg gaccgggccg gcggcggccc ggtctgctgc gacgatcagg
     1441 ggcggtcgga catgcggccg ccggtgccgc cgcccatcat gccgggaccc atgcggcctt
     1501 tcatgccgcg gggaccgacg gcgtgggcgg cgtcgaaggt cttcttctgc tcgggggtga
     1561 gcgtggcgta gaaggtcttg agggcgtcca catgggcaga catctgggct tcgtgggcct
     1621 tcatgcgggt gagcatgcgc tcggcccgtt cgggcgtggg gaggctgcgc cagtcttcgg
     1681 tcggggcggg catcggggtg ggctcggacg ccatcagggc ctgccacgcg ggttcctgtt
     1741 ccggcgtgag cttgagggcc tcgtggaggt gggcgtggtg cagggtgtga cgttccatgc
     1801 ggttgtcgcg ggacgcgccg ggtggggcca tcggaccccc catggggcca cagggcatgt
     1861 ctgccagggc ggggagggcg aggctgccgg caagcagggc gaacagcagg gatggggcgg
     1921 atgggatacg catgaggact ctccgggtgc aagacctgac gactttacgc cgatccgtgg
     1981 cgcgtgggcg atgcgcaggg gggcgccggg tgcagccggg cccggctgcg cggagggcgg
     2041 tgcgtgctaa cggcggaatg ccgactggtc gcgccggggt ggatcggtgc ggacgcccgg
     2101 agcggggctc cgggccctgc gatcctcaat acacgtacag cacgcgcgcc tggaccccat
     2161 tgaccttggg cccgtcctcg accttcacgt cctgggtgta ttgcagttgc acctgccatt
     2221 gcttgtccac gaaggacgcc caggtcagca tggccgtccc gttgttcttg cggccggcga
     2281 tgtcggcgcc gttgagggat tcccgcgcgc cccaggcgtg gcggtaggtg ccggccaggt
     2341 aggtggcgcc gctcaggaag tagcgcaggt gggcgtggcc ctggtacagg ggatccttgc
     2401 gggccttggt gtcacgctgg tcctggtaga actcggtttc gccgatcagg tcgagggtcc
     2461 aggtgtcgga cagcttgcgg atgtagccca cctgcaggtc ggtggcccag cggttgttgc
     2521 tgagggcgaa gccctggttg cggtcgctgc cggtaggggc ggtgaagaac accgaccagc
     2581 ccaggtgttc gccgcgggtc aggtcggcga gggtccacag ggtgccgccg aagatcacgt
     2641 cgcccaggcc gctggtcttg ctgtcggaga ggccgatctt ctgggtgccg aagggtacga
     2701 tgatctgcgg atcgatgatg taatcgccga gcttcatgaa gtggacgtaa cggaagatgc
     2761 cggcgttgat ggacaggtcc agattgctca cggtctggtt gccgtggcta tagaccttgt
     2821 ctgcccgcgg gaactgggcg taacccagga gcagattggt gccagctggc agcccggtgt
     2881 agtcgcccgg atcggggcgg acgtcggcgc gggccgtgcc ggtcagcagg ccgagggcac
     2941 acagggccag gctggcgcgc aggggggagc gatgggtgcg agtgttcatg gacagtctcc
     3001 gtgtcgttgt ggtgtgggtg ctgcgatgcg cttaaaaaag ggccgcggcg tcccggaggg
     3061 aggggcgggg gccgcggcaa ggggaagcgg gccggctggg ggcagcgtga ccgccagtcg
     3121 attcaggggg caccaccagc cggtcgcggg gagggctgct cggacactgc gcgcggtggc
     3181 ttcatggggg ctcccgattg actttgtctg gacggattct ttaatgtgaa acggacttgg
     3241 cgcgatggca gaaccggcgc cccggatcgt ccgggaagga tctgcaccct ccgtgccaag
     3301 cgctgaacgg ccccgcaaga gcgccgtttt tcataaaaaa acatcaggtt ggggcgatcg
     3361 gccgggtgct tgcacgcgga gcgtggggcg cgacgtcgac ttgaaacgag tcaaccggaa
     3421 gttcgcggtt gactcgttcc gcgccgccgg gaggagaccg ccatgggtga gatgcagagg
     3481 gcgttcgacg cgcgcatgct gcgcgcgcgg cgcgcgttct tcgaggacgg cacgcgtccg
     3541 tccggggtga ttccggatgc cgtgctccgg tcctgggagc gctcccgcca acagggcctg
     3601 ggtgtgcgcg accggcgcat ctttatgccg gtgagccgcg aggaggtcgg ccggctgcag
     3661 gcggaaagca gcgagctgat ggcctgcgcc ctgcccgaga tggagcgtct gcaggcggcc
     3721 ctggggcgcg gcgggtgggg catcgtgtgc acggacgcgc aggcgcgggt gctcaaggcg
     3781 gtgtatggcc gcagcaccgc cttccgtgag atggaccgga tcttcgagat cggccgcaat
     3841 ctcagcgagt cggcgatcgg caccaacggg cccggttgcg cgctcatcga gggacggccg
     3901 ctcacggtgc gcgccgcgga acattttctg gacgaaaccc ggcccttcac ctgtgcatcc
     3961 gtgcccctgt tcgggccgga cgggcgcatc gtcggcgtgc tggacgcgac gcgctattgc
     4021 gatggtgcgg cgcccacggt cgtcgagccg ctggccatcg ccgcccgtgc ggtggaaaac
     4081 cgcctgctgg ggcgtctcgg cgccgcgctg aagatcgcct ttcactgtcg cgaagacatg
     4141 ctcggcacgc cgatggaggg tgtgctggtg ttcgacgggg acggcgtgct gcgcggggcc
     4201 aaccaggcgg cacggcagct gctgtcgctg acggcggggg atgacggagg cgtgcgcttc
     4261 gacagcgtgt tcgactgccc gccggagacc tttgggcggt gtctgggcgg gcgggtcgaa
     4321 cggacggtcc tgtggggggg cgagggcatg cgcttcgagc tgcgggtcga ggcagcgcag
     4381 ccgcggcccg tactggccag gccggccagt gtcccctcgc gcgccggctc aggatgggcc
     4441 tccgcgccgt ccgccgcctg tacacgggat gacggcgagg acgccgccgt tgcgcgggcc
     4501 ttgcatgtgg cgcagcgggc ctttgagcgg gacatcccgg tgctggtgct gggcgagacc
     4561 ggcaccggca aggaggtcct ggcccggcgc ctgcacggcg acagcgcgcg tgccgatggc
     4621 cccttcgtgg cgatcaactg ctcgtccctg cccgccgggc tgatcgaatc cgagttcttc
     4681 ggctacgagg acggggcgtt caccggtggc cgtcgcggcg gtgcgccggg caagttcgaa
     4741 ctggcccagg gcggcaccct gttcctcgac gagatcggcg acatgccgct ggagttgcag
     4801 gggcgcctgc tgcgcgtgct ccaggagcgc aacttctcgc gcctgggcgg ggcgcggctg
     4861 atccacttcg acgcccgcat cgtggccgcc acccaccgca agctggacga agcggtggcc
     4921 gccggggcgt tccgggaaga tctgtattac cgcatcaagg gcgtacgcgt gcgcctgccg
     4981 gccttgcgcg agcgggccga ccgtgcggcg ttgatcgata cgctgctacg ccgggaatgc
     5041 ggcggccggc gcccgccgcc cctgtcggcc gaggtccgcg ccgtactgct cggctaccgc
     5101 tggccgggca atctccgcca gctcggccac gtcctgcgtc tggccctgac cctggccgag
     5161 gacgacggcg agatcggcct ccagcatctg cccgaggagc tcctggaggc cgggtcgccg
     5221 acgggcggcg gtctggcacc ggacctgcgg gagcagtgcc tgcgggaggt ggagctcgac
     5281 gccgtcaggg cggcgatggc gcgttgcggc gggaacgtct cagcggcggc gcgcagcctg
     5341 ggcgtggcgc gggcgaccct gtaccgcaag ctgcggatgc tgggcatggt gggcgagggg
     5401 gaggcctgac gccgccaaaa ttccccggcg ccattttttg atccggcgga cgaattttta
     5461 aatggctggc ggggatttaa aaatccggcg cggggatttt taagtgggat ttccggattt
     5521 ctttttgcgg cgggcgcatt tatctgcctc attttttggc aagcaaatcc gtggcgcgca
     5581 ttttttaatc tggctggggg atcttgctgc ctaaaaatga ggcagcaaga tccgatgggc
     5641 ggattttgta gggtcgggat cctttttttt cacggcaggc ccggggcgtg gctgcggtgg
     5701 cgcgcaggcc gatcctgggg cggtgcgcct gggtggtgag tacgcacgga aagggcgtgc
     5761 ccggcgttcc ggcggcgcca tcggcgtgcg gtgagccgcg gccccggtgg caggcgggag
     5821 gagggatcct tcgacatcgc gcgccgcctt tacttccgct caaggctgtt ctaaattgct
     5881 gactgaacgg tcagtaatta atttttccat gagcgaatcc tctccgcccc gccaccaacg
     5941 ccgcaaggag gcgcgccccg ccgaactggc ggcagcggcc ttgtccctgt tcgtcgagaa
     6001 gggctttgcg gccacccgcc tggatgaagt ggcggcccgc gccggcgtgt ccaagggcac
     6061 cttgtacctg tacttcgaca gcaaggaagc cctgttccgg gcggtcatcg aaggcagcgt
     6121 ggtgcccctg gtggagcaga gcgaggcgcg catcgaggcc ctgaagcacg acccggagcg
     6181 tctgctgcgg gagaccctgg tgaattggtg gttcaacttc ggggccaccg agctgggcgg
     6241 catctgcaag ctgatgatct ccgaggccgg caatttcccc gaggtggcgg cctactacaa
     6301 cgaggcggtc atcgtgcgct ggatgaacct gctcggccgc gtgctggaac tgggcatgga
     6361 gcagggcgtg ttccgtcgcc tgaatgtgga catcctgcgc cagcaggtct tcttcccgct
     6421 gctgatgcag tccgtgtgga agaactccct ggcgggctgc tgcgcgccgg tggcgctgcc
     6481 ggacagctat ttcgacacct atttcgatct cgtcttccgt ggcctgctgg ccgcgccgcc
     6541 gctggagcct tcccgatgat gcgtcccctt gtccttgccg tcgcggtggc gacggtcctt
     6601 gccgcgtgca gcaaaccgga gcccgtggcc gaagcgcccc gtccggtggt ggcgcagaag
     6661 ctcacgcccg tggcggtggc ctcgggcgcc ctgtacaccg gcgaggtgcg ggcccgctac
     6721 gagtcggacc tggccttccg gatcaccggc aagatcgtcg agcgcaaggt ggatgtgggt
     6781 gccgtggtca agcccggtca ggtgctggcg cggctcgatc cggccgacgc ggggctcaat
     6841 gcggaggcgg cgcgggccca gctggccgcg gcgcagaccg atctcgcctt tgccaaggcc
     6901 gaggtggagc gctaccggga tctggtggcc aaacatttcg tcagccgcag tgtgctggac
     6961 gccaaggagg cgaccttcaa cgcggcggcc gcgcgcgtcg cccaggcccg cgcccaggcc
     7021 agcgtggccg gcaatcaggc gggctacacc acgttggtgg cggaccaggc cggggtgatc
     7081 acggcgatcc tcgccgaggc ggggcaggtg gtgaaggact ccgcggtggt gatgcgtctg
     7141 gcccgggacg gggagcggga ggtgctcatc gcggtgccgg aaagccgtgt cgccgccctg
     7201 cgcaaggcgg ggcaggtgga gatccgcctg tgggcccagc cggagcgggc gctggaaggc
     7261 cgcgtccgcg agatcgcgcc gagcgccgat gcggcgacgc ggacctacgc ggtgaaggtg
     7321 acgatcctgt ccaacgatcc gaccgtccag ctgggcatga ccgccaacgt gatcctcccc
     7381 gatgcggccg ccggcgccct gctggtgccg tccacggcgg tcttcgagca cgcgggcggc
     7441 agcgccgtgt gggtgctgca ggccgatggc gagctggtca agccggtgct gcggccggtg
     7501 acggtgaagc agtaccggga ggacggggtg atcctgtccg gcggcgtgca ggcgggcgag
     7561 accgtcgcgg tggccggcgt gcacaagatc gtcgccggcc agcccctgcg cccggtggcg
     7621 ccggcccgtc cgggggccgg ggcatgaagc gtttcaacct gtcggagtgg gcgctgcgcc
     7681 accaggccct ggtgcgttac ctgatcgtcg ccctggccct ggtgggggtg atggcctatc
     7741 agcgcctggg gcagtcggag gatccgccct tcaccttcaa ggtcatggtg gtgcgcaccg
     7801 aatggcccgg cgcctcggcg cgggagatcg aggagcaggt caccgaccgc atcgagaaga
     7861 agctccagga agtgcccttc gtggactacc tgcgcagcta ttcgcggccg ggcgaatccg
     7921 tggtgctctt cgtggccaag gattcggcgc cggcgtcggc gattcccgac gtgttctacc
     7981 aggtgcgcaa gaagatcggc gacatcaagg gcatgctgcc gacgggcatc cgcgggccgt
     8041 atttcaatga cgagttcggc gacgtcttcg gcaacatcta cgccctgacc ggcgacggct
     8101 tcgactacgc ggccctcaag agcgcgtccg agacgatccg cgccgagctg ctgcgggtgc
     8161 ccaacgtggc caaggtggat ctgttcggcg agcaggaaca gcgcatctac gtggagctct
     8221 ccaacaccaa gctcgccacc ctcgggctgg acgtgcagac ggtcaccgcg gccctcgccc
     8281 agcagaacac ggtggcgccg ggcggctatt tcgagacccg cgacgaccgc atctacctgc
     8341 gcccgagcgg cgcctacgac accgtcgagg cgatccgcga gacgcgcatc cgcgctggcg
     8401 ggcgggaatt ccgcatcggc gacatcgcca cggtggcgcg gggcttcgtc gatccgccgg
     8461 cgccgcgctt ccgctaccgt ggccacgacg ggctgggcct gggcgtgtcc atggccaagg
     8521 gcggcgacat catcgccctg gggcagaacc tcaaggaggc ggtcgcgcgc atccaggccc
     8581 agctgccggt gggcatggag ctggccgagg tggcgagcca gcccgaggcg gtgcagcgct
     8641 ccatcagcga gtttgtgcat tccctggcgg aggccgtgat catcgtgctg gtggtgtgct
     8701 tcttctcact gggcctgcgc accgggctgg tggtggccct gtcgatcccc ctggtgctgg
     8761 cggtcacctt cttcggcatg cgtctgttcg acgtgggcct gcacaagatc tccctgggcg
     8821 ccctgatcct ggccctgggc ctgctggtgg acgacgccat catcgccgtg gagatgatgc
     8881 tggtgaagat ggagcagggc tgggaacggg cccgcgctgc ggcctacgcc tacaccagca
     8941 ccgccgggcc gatgctgtcg ggcacgctgg tgacggtggc gggctttctg cccatcgcca
     9001 ccgcccagtc gtccaccggc gaatacaccc gttcgatctt ccaggtgaac gccattgcgc
     9061 tgctggcgtc gtggctcgcc gcggtgatcg tggtgcccta tctcggttac gtgttgctgc
     9121 cggacccccg caaggccgcc ggaccgggct ggctggaacg gcgcgtgccg gccttcgcac
     9181 ggctcaaggc gcggctcggc atcggcggcc acacgcatca cgatggtgac gaggacgcgg
     9241 tctttcagac gcccttctac aaccgcttcc gagcgctggt agacagatgc gtggcgcggc
     9301 gctggtgggt catcggcgcg acgctggcga tcttcgtgct ggccctggtg ggcttcggtc
     9361 aggtgcagca gcagttcttc cccaattcca cgcggctgga gatcctggtg gatctgcgcc
     9421 tgcccgaggg cagctccctg cgcgccaccc aggcggaggc agccaggctg gaggcgatcc
     9481 tcgaccggga gcccggcatc gccaactatg tgacctacgt gggcaccggc agcccgcgct
     9541 tctatctgcc cctggaccag caactgccgg ccgccaactt tgcccagctt gtgatcctca
     9601 ccgacagcat cccggcgcgg gaggcgctga ggcagaggct gatcgagctg ttccgtacgg
     9661 atttcccggg tctgcgggcc aacgtgacgc gcctggagaa cgggccgccg gtgggcttcc
     9721 cggtgcagtt ccgggtgagc gggccggaca tccccacggt gcgccggttt gccgccgaag
     9781 tggccgaggt gatgcgcgcc aatccgaacc tctcggacgt gcagctggac tgggacgagc
     9841 agtccaaggt gctgcgcgtc gaggtggatc agcacaaggc gcgcctgctg gggctgtcta
     9901 cccaggacgt ggccaacttc ctcaacacct ctttgcgcgg cctcacggcc accagctacc
     9961 gggaaggcat cgagtccatc gacgtggtgc tgcggggcga cgccttcgag cgggacaagc
    10021 tgtccctggt gtcggagctg tccatcccca cccgcgccgg caagtccgtg cccctgggcc
    10081 aggtggcgac ggtgagctac gcctgggagc agggcctgat ctggcgccgt gaccgtctgc
    10141 gcagcatcac tgcccgcggc cagatctacg acagccgcct gcaggcgccg accgtcaccg
    10201 cccaggtcga gccgctgctg gcgccgatca aggcgcgcct gccggccggc tatcgcatcg
    10261 acaccggcgg cgcggtagag gagagcgcca aagggcagag ctccgtgaat gcgggcttcc
    10321 cgctgttctt cgcggtggtg tttaccgtgc tgatggtgca gttgaacagc ttctcgcgga
    10381 ccaccatggt ggtgctcacc gcgcccctcg ggctgatcgg cgtggtgctg ttcctgctgc
    10441 tcttcaacca gcccttcggc tttgtcgcca tgctcggcac catcgccctg ttcggcatga
    10501 tcatgcgcaa ctcggtgatc ctggtggacc agatcgagca ggacgtggag aaggggcgca
    10561 gcgtgtggaa cgccatcgtg gaatcggccg tgcgccgcct gcggcccatc gtgctcaccg
    10621 ctgcggcggc tgtgctggcg atgatcccgc tgacccgcag cgctttcttc gggccgatgg
    10681 cggtggccat catgggcggc ctgatcgtcg ccaccgcgct gacgctgctc ttcgtgccgg
    10741 ccctgtacgc cgcctggtat cgggtgcgtc gatgacgcgt tcccgcgcaa gtacgctaga
    10801 attggaaggt ttttccattc acgctggcag gccgatcctc ccgtccgggc cgcggcggat
    10861 ctttacaggg gagttccaat gaatttcgaa caagcgcgct tcaacatggt cgagcaacag
    10921 atccgcccct gggaggtgct ggatttctcc gtgctggacc tgctgatgag catccgccgc
    10981 gaggagttcg tgcccgaggc ctacaagtcc gtggccctct ccgaggcgcc gatccccctc
    11041 ggccacggcg cgagcatgct ggtgccggtc atcgagggca aggtgctgca ggcgatccag
    11101 gtcaagggtt ccgacaaggt cctcgaagtg ggtgccggct ccggctattt cgccgccctg
    11161 ctggccgcca aggccgagtg ggtgcgcacc gtcgagatcg agcccgcact ggtgaacatg
    11221 gcccatgaca acctgaagcg ctatggcgtc gagaacgtca tcgtcgagga aggcgacgcc
    11281 gtgagcggtt gggccggcaa tgccccctac gacctgatcg tggtctccgg cgctgtgccc
    11341 cacgtgcccg aatccctgct ggcccaggtg aaggtgggtg ggcgcctgtt cgccttcgtc
    11401 ggcgggccga ccctgcaggt cgccaccctg atccaagtgg tgggcgaggg ccagtaccgc
    11461 accgagaaag tcttcgagac ccaggtgccg atgatgcgca acgccccgcg caagaaccag
    11521 ttccagttct gagcggacgc ggcgatgcag cagatcctgc ctgcgcaact ggacgagtgg
    11581 ctgcgggatg cgtcccgccc gcagcccgtg cttctcgatg tccgtgagcc ccacgaattc
    11641 cagtactgcg ccctcgccgg gtccctgtcc atgcccatgg ccagcgtgcc ggcgcgccag
    11701 caggagctcg atccggatgc ggagaccgtc gtgatctgcc accacggcat gcgcagcatg
    11761 caggtggccc tgttcctgga gcgccagggc ttcgggaagg tgatcaacct gaccggcggg
    11821 gtcgccctgt gggcccaaca ggtcgacccg tcgatgcccc agtactgaca tcaccaggtg
    11881 aagggaatga gcttgagcaa ggttcggttt gcagtgctgg cgtgggccgg cgtgatggcg
    11941 tccggtgcgc aggccgtcga tctggagcag gcctaccggg acgcggtgac ctacgacgca
    12001 accctctccg gtgcgcgggc ctcccttgcc gccgggcgtg agaagctgcc ccagggccgc
    12061 gccggcctgc tgccgacgat ctccctgagc ggcaacacca actacaacac ggcggtcttc
    12121 aataacctcg acccccattt caccagcaag tacaacagtc acagctggat cgcccagctc
    12181 aaccagcccc tgttccgctg gcagaactgg gtccagttca agcagggcga gctcaatgtg
    12241 ggcctggcgg aagcccagtt caagctggcc gagcaggatc tcgtcctgcg cgtcgctcag
    12301 gcttatttcg acgtgctggc cgcccaggac accctggtgg ccgcctcggc cctgcgcgac
    12361 gccaatgccc agcagctggc gctcgccaag aagagcttcg aggtcggcac cgtgaccatc
    12421 accgacgtgc acgaggccca gtcccgttac gatctgtcct cggcccagac catcgcggcc
    12481 gagagcgacc tggaagtccg ccgcaacgcg ctggctgtgc tcaccggcaa gacgccggac
    12541 gccctcaaga ccctgcgcaa gggcgtggcc ctgtccagcc ccgagccggc ggacatgccg
    12601 tcctgggtgt ccgcgtccga agccaccgcc ctgcaggtgc aggcggccca gctcggcgtc
    12661 gagatcgccg cgcgggaaat cgagcgcaac cgtgccggcc acgcgcccac cgtcgatctg
    12721 gtggcgagtt acggcaactc ggtcaatccg gtcgcctcgg tgacgcccac caccaactac
    12781 gattcccgcg ccatcggcct gcaggtggcc atccccctgt accagggcgg gggcgtcaat
    12841 tcccgcgtac gtgaggccat cgccctgcgc gacaaggccg ccgcggatct ggacaacgcc
    12901 cgtcgcactg ccgcgcaggc ggcccgtcag gcctatctgg gcgtgaccag cggtctcgcc
    12961 caggtgcgcg ccctggaggc cgcccaggtc tcgtcccgct ccgcactgga cgccaacaag
    13021 ctgggctacg aggtcggcgt gcgcatcaac attgacgtgc tcaacgccca gagccaggtc
    13081 ttcgacaccg agcagaagct ctccaaggcg cgctacgaca cgctgatggc ccagctcaag
    13141 ctcaaggcgg ccgccggcac cctgggggac gacgacgtcc gcgccctcaa cgccctgctg
    13201 gactgagcca cgcgccggga gcccgtgccg ccgacggccg ctttgcggcc gtttccattt
    13261 gcggtcaatc cgtgaccgcc gtccgggccg ttccgtttat ccttccttca ttccgacgtt
    13321 ccgacttccg aaccgattcc catgcgttac accgacctgc gtgacttcat cgcccagctc
    13381 gagcgcctcg gcgagctcaa acgcgtccgc gtccccgtcg atccccatct cgagatgacc
    13441 gagatctgcg accgggtgct gcgcgccggc ggcccggcga tcctcttcga acagcccacg
    13501 gggcactcgg tgccggtgct ggccaacctc ttcggcacgc cccagcgtgt ggccctgggc
    13561 atgggcgaga gcggcgcgga ctggcaatcc gctttgcgcg aggtgggcaa gctgctggcc
    13621 ttcctcaagg agccggagcc gccccgcggc ctcaaggacg cctgggacaa gctgccggtg
    13681 ttccgccagg tgctcaacat gtcgcccaag acggtctcgt cggggccctg ccaggaggtt
    13741 gtccgggaag gccgcgacgt ggatctggcc agcctgccca tccagcattg ctggccgggc
    13801 gacgtggcgc ccctgatcac ctggggcctg gtcgtgaccc gtgggcccca caagccgcgc
    13861 cagaacctgg ggatctaccg ccagcaggtg ctcggcccca acaaggtgat catgcgctgg
    13921 ctggcccatc ggggcggcgc actcgatttt cgcgaccacc agcaggccca tccgggcgag
    13981 cccttcccgg tggcggtggt gctgggctgc gatcccgcca ccatcctcgg cgcggtgacg
    14041 ccggtgccgg attccctgtc ggaataccag ttcgccggcc tgctgcgggg cgccaagacc
    14101 gagctggtga aatgcctggg gtcggacctg caggtgccgg cctccgccga gatcgtcctc
    14161 gaaggcgtca tccatccggg cgagacggcg ctggaaggcc cctacggcga tcacaccggc
    14221 tattacaacg agcaggccga gtttccggtc ttcaccatcg agcgcatcac ccaccggcgc
    14281 gacccgatct accacagcac ctacaccggc aagccgcccg acgagccggc catgctcggg
    14341 ctggccctca acgaggtctt cgtgccgctg ctgcagaagc agttcccgga gatcgtggac
    14401 ttctacctgc cgccggaagg ctgctcctac cgcctcgccg tggtgagcat ccgcaagcag
    14461 taccccggcc acgccaagcg ggtgatgttc ggcatctgga gcttcctgcg ccagttcatg
    14521 tacaccaagt tcatcatcgt ggtggacgag gatgtgaaca tccgcgactg gaaggaagtg
    14581 atctgggccc tgaccacccg catggatgcc acccgcgaca ccacgctggt ggaccatacg
    14641 cccatcgact acctggattt cgccagcccg gtggcgggtc ttgggtcgaa gatgggcctg
    14701 gatgccacca acaagtggcc cggtgaaacc cagcgggaat gggggcgcgc catcgtcatg
    14761 gatgcggccg tcaaggcccg tgtcgatgcc ctgtgggcgc agctcggcct gtaggtgtcc
    14821 ttcgccggtc ccttcggccc ggcccgctct tagtctgact cgaggtgtcc atgttgtgcc
    14881 gtatgctcgc ccgcgccctg ttgagcgcgg gggtgatcct gatgcccgca ttcgcgtccg
    14941 ccgacctcgt cggcgccggc gcagtccagt tcgaaaccga ccgcctggcc gaagggcagg
    15001 ctgcgctgcg gagccgccag ctggagcggg ccgagcgcgc cttcagtgaa gcgctgcggg
    15061 ccaacgccgg ttccaccgac gccctgatcg gcctcgccca gaccgcccag ttgcaggggc
    15121 ggcccgacaa ggcgcgggac tggatggcca aggccgtggc agcggcgccg ggttcaccgc
    15181 caatcctgca ggcccagtcc aagctgctgg tggagcaggg catgggcgcc ctggccgagg
    15241 agcactaccg tcgtgccatc gccgccaatc ccggcaatcc ccagttcagc caggatctgg
    15301 cgagcctcta tctggagcag ctcaagcgtc ccgcggcggc ggtcacggtg ctccgcgacc
    15361 tcctccgtcg ccagcccgag ctggcctcgg cctacctcgg gctgggcctg gccctggccg
    15421 cggacggcaa gctcgacgaa gccggtcgca cgcttgacga agccgtccag cgcgatccgg
    15481 tgaatccctt cgcctaccat gcccaggggc tgctggccct caagcagggg cgcgcggatg
    15541 cagcgctgac ggccttcgac aaggcgctcg ccctgcgccc cgagtttgtc ggcgcgctcc
    15601 tcggcagggg cgatgcgctc ctggccctgg gcaaggccga tggggcgctg gcgacctatc
    15661 gccgtgccgc gtcgctggcg ccgggcagtg cggtgccgat cgccaagcgg gcccaggcgc
    15721 tggagcgcct gaagcgtccg gatgaagcgg aagcggcgta tcgcgaggcg ctcaagctgg
    15781 agccgaccca cacggtcgtc ctgaacaatc tcgccttcct gctagcgtcc cgaaagcagc
    15841 ggctcgacga ggcgctcggg ctggcccagc gggccacgac gctggatccg gcgcgggccg
    15901 atcacttcga tacgctgggg gtggtgctgc tggcgcgggg cgatcaggcc ggcgcgcgca
    15961 aggccttcga gcgggccgtg gcggtcgatc ccgcccacgc cggcgcacgt cagcacctgg
    16021 cccgtgtcgg cgcgccggcg gaggcgcccg cggcggcggg gccttccgcg cctgccgctt
    16081 caccgcggga gatcccgaag gaggccgccc ggagcagcac ggacgatgct gcccgggccc
    16141 tctccacgcg ccttgaggcg tggcgacagg cgtgggaatc caaggatgcc ggacgttacc
    16201 tgagtttcta cgggaaagat ttcgcaccgc ccgacggacg cgaccgcggc acctgggaag
    16261 ccgagcggcg ggtccgcctc gacaagaagg gcgagattca cgtgcagatc gttgcccccg
    16321 aactgcgggt ggagggcgac acggcggcgg tcagcttcga gcagcgctac cgggccggtg
    16381 cgctggtgga cacgggccgc aaacgcatgg cgtggcaccg ggaaggcggg gaatggatga
    16441 tccgccggga aggcatgcag tagcggcgcg cgtgttcaga cggcgctggc gagccgccag
    16501 gcgttgccgc cgcgccgttt ggcagcatac atggcggcgt cggcggccgc ttcccaggca
    16561 tcggcactgg ggtaaggacc ggggatcacc gccgcgccga tgctgccggt gacgccgtaa
    16621 cggcgcccgt cttcggtggt gagcgcggcg atttccgcga gcagccgctc acacagtggg
    16681 ccgatgtcgt tctcggcgga aatgtccagg cagatcagcg cgaattcatc cccacccagg
    16741 cgtcccacca gatcggtatt gcgcgcaacc tgccgcaggc gttcggacac ggccgccagc
    16801 agcagatcgc ccacggcgtg cccgaaggtg tcgttgatcg gcttgaagcc gtccaggtcg
    16861 atcagcagca gggtcgcgct gcgctgccag cgcacggcgg cgtcgtgggt gctgtcgcgg
    16921 gcttcccggt aggcggtccg gctggccagg ccggtcatcg ggtcgaattc ggcaaggcgc
    16981 cgccagcgct ggctttcctg ggtcagattg cgatagcgcc gtaccaggcc gacgacgatc
    17041 accgtgaagt tcccgagggc caggacgaag gcgccaatct ggatgctgcg cagggtgcgg
    17101 gtgcgttgca cggacgcctg ttccagtgcc gaggtgaggc ggttcatttc agccaggatg
    17161 tcccggttgt gctgcaccat gtagtgcgtg gcttcaccca gttcggaatc tgccaggggc
    17221 ttgccggcga ggaattcggg caggcgcgcg tccagcggcg ccatcagggt cgccacccgt
    17281 tcgacggcat cgagtccggc gcccgggtcg atcggccgca ggtgcacgga cgggccggtg
    17341 ccgccggccg tgtcgccgcc atcgtggaag gcacggaggg tccgttcaaa gaggcgatag
    17401 gcggtacgca gttcctgttc tgcatcgcga cgggcatcgg ccgaggggac gtggaccgcc
    17461 aggagcagcg ccttggtgat ctcctgggac agcatgcgct gacggccggc caggttgatg
    17521 gccaccgcat cttccgccac ctgggcggcg atccagacat tgatcagcag cacggccaga
    17581 tcgagcacca cgaacaggac gatggcgctg aggaggcggc gcggcgaggc gatgagctgg
    17641 tcgatgcgaa taaacatggg agtccgcacg gcggagaggg acgtctcatg tcggtcaacg
    17701 accgcgaatt tggtggcttt agtaaatgac gctggaaaat tgtcatgaaa aagggcggct
    17761 ccagagagcc gccctattgg attcccgccg gggagcgatc aatccttgaa gcgttcttcc
    17821 agggccgcga tggcgggcag ggtcttgcct tcgaggaatt cgaggaaggc accgccaccc
    17881 gtggaaatgt agcccacgtc gtcggcgatg tggaacttgg cgatggccgc cagggtgtcg
    17941 ccgccgcccg cgatggaaaa ggcttcggag tgggcgatgg cggaggccat catcttggtg
    18001 ccgccggcga actggggcag ctcgaagacg cctaccgggc cgttccagac gatggtgccc
    18061 gcgttggcga tgatctggga gagctgcgca gcggccttgg gaccgatgtc gagaatgcgg
    18121 tcgtgggggc cgacctcatc cacgggcact ttgttggcgc gggccatggc agagacttcg
    18181 tcggccacga ccacgtcttc gggcaggggc acttcggcgc cacgggcctt catcaggtcg
    18241 atgatgacct gggcttcctt gaccagctcg ggctcggcga gggattcgcc gatgcgcttg
    18301 ccggaggcca gcaggaaggt gttggcgatg ccgccgccga cgatgagctg gtccaccttg
    18361 tccgccaggg acttgaggat ggtcagcttg gaggacacct tggagccgcc gacgatggcc
    18421 accagcggac gcttggggct gtgcagggcc ttgcccaggg cgtcgatttc ggcgcccatc
    18481 aggatgccgg cgcaggccgt gggggcaaag cgggcgatgc cgtgggtggt ggcttcggcc
    18541 cggtgggcgg tgccgaaggc gtcgttcaca tagacgtcgc agagggcggc catcttgcgg
    18601 gccagttcct cgttgtcctt cttttcgccc ttgttgcagc ggcagttttc cagcagcacg
    18661 acttcgcccg gggcgacgtc gaaaccaccg tccacccagt tctggatcag gcgcacgggc
    18721 ttgcccagga gctggcccag acgcacggcg acgggcgcca gggtgtcctc ggccttgagt
    18781 tcgccttcgg tgggacgtcc caggtgggac gtgaccatga ccgcggcgcc gccttcgacg
    18841 gcgtagcgga tggacgggat ggaggcgcgg atgcgggtgt cttcggtgat gttgccggcg
    18901 tcgtcctggg gtacgttgag gtcggcacgg atgaagacct tcttgccctt gacgtcgaga
    18961 tcggtgagtt tcttgacttg catgatgtgg ttctacggag cgtcggaatg aaaaacgccc
    19021 cgcggagcgg ggcgtcggtg aggcggatga tcagacgttc atccaggcgc gggcggcgtc
    19081 cagcatgcgg caggagtagc cccactcgtt gtcgtaccag gccagcacct tgaccagcac
    19141 ggagccgtct tcacccttga tgacgcgggt ctgggtggcg tcgaaggtgg aggagacggt
    19201 ggtgtggttg aagtcggagg a
//
LOCUS       DAOQPA010000068        19208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1433_length_19208_cov_19.497624, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000068 DAOQPA010000000
VERSION     DAOQPA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19208
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1433_length_19208_cov_19.497624"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKX01_10530"
     CDS             <1..234
                     /locus_tag="PKX01_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HMZ76606.1"
                     /translation="LSDPLLAGLKQSPRPVSGRCGACRYLAMCNGNTRVRAWQLTGDP
                     WQEDPGCYLDNAEIGVADGERLEVKPWVRLQPA"
     gene            626..2185
                     /locus_tag="PKX01_10535"
     CDS             626..2185
                     /locus_tag="PKX01_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HMZ76607.1"
                     /translation="MKRLLASLAFAATLLPTVAAAVVDAPALYTQHCASCHGPGRLGA
                     MGPALLPESLERLRRAEAIATLRDGRTATQMPAFGAILSAAEIEALADWVRTPVSPAP
                     AWSEDDIRASRVEPFPRGSLPDAPQGPVAQTDPMNLFIVVEAGDHHVSVLDGDRMTRI
                     HRFPSRYALHGGPKFTPDGRYVFFASRDGWVSKFDLWNLKVVAEVRAGLNTRNAAVSA
                     DGRFVAVANYLPGNVAIFDADLKLLRLIPARTLKGDASSRVAAVYDAPPRRSFIVGLK
                     DVAEIWEVSYDPDAKPIFDGYVHDYKMGEAMALPGFLNSRRTALDTPLDDFFFTQDYA
                     NVVGASREGRGEVVNLDVRRAIATLDLPGMPHLGSGISWMRDGRRVVASTHLKEGRVS
                     VIDLQRWQTVATIPTLGPGFFLRSHENSPYAWVDSMMSPTAKDTLQVIDKQRLAVVAQ
                     VRPEPGRTFAHVEFDRHGRYVIASLMENDGALIFLDATTFQEIKRLPASKPIGKYNLF
                     NKIGKSEGTSH"
     gene            complement(2315..2845)
                     /locus_tag="PKX01_10540"
     CDS             complement(2315..2845)
                     /locus_tag="PKX01_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76608.1"
                     /translation="MKEGTVLLVSCAATAALWLWAEQNRPAPALPEAAPTPSAEAPPV
                     ASPPVAPPSPDSRAYLCIANGQRSYSDRPCAAGERSERVNIQAPPPGSPTPSIQEQYA
                     DLVANRPRVTPGAAEPLVIPRDPDHPEKAGLCEELWLHIREIDEALRQPTPPQQAETL
                     RALRLRYQNDRFTQGC"
     gene            2998..3234
                     /locus_tag="PKX01_10545"
     CDS             2998..3234
                     /locus_tag="PKX01_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ76609.1"
                     /translation="MSETLAPVIVDARGLMPPQPMHLTLEALDTLPAGGEVILLLYRE
                     PTPLYDVLIRNGYVYRAESTPGGEFAIHIRHRTD"
     gene            complement(3245..3937)
                     /locus_tag="PKX01_10550"
     CDS             complement(3245..3937)
                     /locus_tag="PKX01_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ76610.1"
                     /translation="MSPDKIDIPGLLSRMPMFSALSPDDLAHIAAATRERRLDKGERL
                     FQRGDAPKGFFLVVWGQVKLAFTSSQGNEKVVEILGPLQSFGEAVMFLDQPYPVLAEA
                     LADALILHIGSAAVFGLIDADPKFARKLLAGLSIRLHSMVRDVESYALRSSTQRVIGY
                     LLQLAESTPCAAQGERSVELPTSKQVIASRLSLTPETLSRIFHELSGAGLISVHGKQI
                     VIHDAERLAAHD"
     gene            4213..6525
                     /locus_tag="PKX01_10555"
     CDS             4213..6525
                     /locus_tag="PKX01_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76611.1"
                     /translation="MKSAADSQTGPGASREDALSPDATLFEALSLFLAGGPSYLPVGD
                     AGPARRYLGAATLLGALHAGLSGDTPVSRLNLDTSADSATAAGSAKTLIDALPLLLWM
                     KDAEGRYLIVNRSFAEACGRSTSAEVIGRADDELWPAGRAALHAAADAEVLAGGEAMR
                     AVDQVSGGASPVWLESVRAPLRGAEGGIVGTVGFAVDVSERIRLLAAAERSEKDLNFV
                     LSAIGEGVWDRDLASGTVRHNRRWCELLGLGHAQLIHSVDDLLAMIHEADRPLYNAAV
                     AACMAQHGAYICEYRMRRPDGRVLWVRDRGDVVEFDADGQPSRMMGSIADITEVKESQ
                     RHLEHMAYHDTLTQLPNRSLFYDRMQMAVAQAERNGTLAAIAYLDLDGFKAVNDSLGH
                     AVGDRLLVVIARRLMASTRAGDTVARIGGDEFALIYNGLERSEECEQILQRLLASIGE
                     PVTIDNRELTVTASVGVTLCPLDGSDPEHLLLHADEAMYLAKESGRNAFQLYDPGRDR
                     RLRALRDAQHRVELALDGGELELHYQPKVDVLTNALTGVEALIRWKHPELGLRMPGEF
                     LPAVKDSRFEIELGDWVLRTAVKQLDAWHREGLSTPMSVNIAARHLVHPEFVANLRRL
                     LAAYPAVHPELLQLEVLETATLGDVKRVAGIMAACNELGVSFAIDDFGTGQGALSSLR
                     RLPVRSIKIDRSFIHNMLEDEEDLATVQSVIGLGAAFHREVIAEGVETPGHRQALLRL
                     GCRMIQGHGVARPMPAARMQRWMQEHRTQH"
     gene            6672..7433
                     /locus_tag="PKX01_10560"
     CDS             6672..7433
                     /locus_tag="PKX01_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ76612.1"
                     /translation="MEFLTDPQLLIAFLTLTALELVLGIDNVIFISILVDKLPPERRA
                     FTRRLGLFLAMFMRIGLLMILSWLAGLTEPLLTVLGHGFSGRDLILLGGGLFLVWKST
                     MEVHQLMEGEEGEASSTVQATFGAVLLQIVLIDIVFSLDSIITAIGMVNNLTVMIAAV
                     IASVGLMMVASGPIGDFVSRHPTVKMLALSFLLVIGVVLIADAFGQHVPKGYIYSAMA
                     FSVMVEMLNLRMRSRQAAEPVKLHEPYVREPGRDA"
     gene            7430..8434
                     /locus_tag="PKX01_10565"
     CDS             7430..8434
                     /locus_tag="PKX01_10565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HMZ76613.1"
                     /translation="MSAGDGAAAPAPFGPLTPDFVLAAVESAGLFCDGRLLTLNSYEN
                     RVYQVGIEDAAPLVAKFYRPGRWSDAAILEEHTFLAELAAAEIPAVPALELDGRTLHA
                     FEGFRFALFPRRGGRAPELDDPAVLTWLGRFLGRIHAVGATRPFVHRPDLTPRSFGEE
                     ARDALLETAFIPAELRATWQSVVDQALAGVHACYARAGELPTIRLHGDCHGGNVLWTD
                     AGPHFVDFDDARNGPAIQDLWMLLSGDAPAMARQLDHVLEGYEAFADFDPRSLHLVEA
                     LRTLRLIHYAAWIARRWDDPAFPAAFGWFDSPRYWQDMILALREQVALMDESPLTRQF
                     "
     gene            complement(8502..9008)
                     /locus_tag="PKX01_10570"
     CDS             complement(8502..9008)
                     /locus_tag="PKX01_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HMZ76614.1"
                     /translation="MHQTEVSPPAAAGPGPVVVFLDFDGVTHPWGESEDFRCLPVFEA
                     VLRDYPEVRVVIASDWRTLFSLPKLTARFAEDLRARVVDVSPTIFPKEANELYGLRER
                     EARQWLARHPDIAGWLAVDDAPGNWPTRSRLVLTDFKRGFTEEDAGRMRRMLDGFRRG
                     EYPEQAAA"
     gene            9147..9926
                     /gene="fabI"
                     /locus_tag="PKX01_10575"
     CDS             9147..9926
                     /gene="fabI"
                     /locus_tag="PKX01_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HMZ76615.1"
                     /translation="MQPIINLAGKVGLITGIANEKSISTGVAEACIEAGAKVVITYQN
                     DKTLAFIEPIVARLGIEDVYLLDVTKPETMDDVFAQIDAKHGKLDFAIHSMAFAKRDD
                     LHGRVVDTSPDGFALAMDVSTHSFVRLARKAEPLFEKAGGGSLITMTYLGSEKVIANY
                     GVMGLCKAALEAATRYMAFELGPKNIRVNAISPGPLMTRAASGIAGFDGLMAAAVERS
                     PLHRLVTPEDIGALTAFLVSDAGRNLSGGVHFVDAGYNIIA"
     gene            9935..10522
                     /locus_tag="PKX01_10580"
     CDS             9935..10522
                     /locus_tag="PKX01_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76616.1"
                     /translation="MAVRPDRDAASYDRRIADPVPDHPLLAGLAELGRGESSIVLDAG
                     DGERVFKVVSSPADYCFLAADDRPIGLHFPRLIKDHGVVGKASNGYSFRLLELERLLP
                     VAGEAEALGQLLSDAYWQGCEKWANLGVNRGRLALYHIAQEPPPGLPTSLVKAVADLS
                     DFIEGYPVQPDILNPHNLMMRPDGTLVFSDPVFLP"
     gene            10608..10787
                     /locus_tag="PKX01_10585"
     CDS             10608..10787
                     /locus_tag="PKX01_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76617.1"
                     /translation="MLPTPFERIKDLIGKIHALEDKLMTTGILPLSEQAKIHEQIRHY
                     KEEIALLERRTRKHS"
     gene            10947..11525
                     /locus_tag="PKX01_10590"
     CDS             10947..11525
                     /locus_tag="PKX01_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HMZ76618.1"
                     /translation="MSGDHFHVHGPHDHAVEHAAEHGGGDPFASRIAAMTAILATLGA
                     LFSYAGGATQNDALMHKNEAAIRKTEASNRWGYYQAKSAKQNLAELGVSLTTGEIQTR
                     YTAEIERYKQEKQEIKSAAEELEKAAIEAEEASEHAMHAHHRWAQAMTAMQVAISLAA
                     ITLLTRRRWMQWTSVGVATAGCAIAGLALAGI"
     gene            complement(11575..12480)
                     /locus_tag="PKX01_10595"
     CDS             complement(11575..12480)
                     /locus_tag="PKX01_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HMZ76619.1"
                     /translation="MWFRNLQLYRLPENWDLSADTLADKLAARPFQPCGSQDMASRGW
                     TSPTRDDRFVLAVGGHYLIALAVEQKLLPSSVVNQVAADKAELIEAEQGYKPGRKQMK
                     EIKEAVLQELLPRAFTRRRTMWAWIDPKGGWLAVDAASPARAEDVLDALRDTLDALPV
                     KLLKTQQSPVSAMTGWLAGGEAPGAFTIDQDCELRAVTDEKAAVRYVRHALDGQDVQD
                     HLAAGKLPTRLALTFDDRIALLLTEKLEIKRIAFLDVVKEEAERQAETADLQMEADFT
                     LMTGELSRLIPALLEALGGEQKDAF"
     gene            complement(12562..12918)
                     /locus_tag="PKX01_10600"
     CDS             complement(12562..12918)
                     /locus_tag="PKX01_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76620.1"
                     /translation="MKVLVVDDQRVNRTLPAVLLRQQGCEVLEAESGEDALRLIDSHG
                     FQAILLDVSMPGISGTELCRQLRSRPEFGGVRIIAYTAHAMPGEREDILSAGFDDILI
                     KPINRQVLLAALRLPA"
     gene            complement(12915..14618)
                     /locus_tag="PKX01_10605"
     CDS             complement(12915..14618)
                     /locus_tag="PKX01_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76621.1"
                     /translation="MSSPNPPGSPSSRRLHGPIVALVIALIVLIWGAILYKIHTEEEL
                     VIHSVNVENLNLARAFEEHTIRTLKSVDQAVLFLKFQYEKYGDKVDIGEYVREGMIIS
                     TIFNQLGVIDEHGIYILSNLPNHKKIDLSDREHFKVHMERDTNQLFISKPVLGRASKK
                     WSIQMTRRINKPDGSFGGVVVISLDPMYFSDFYKGVDLGTHGAVALVGLDGIVRARRS
                     GNNDSVGQDISASPFFTQTNTGTTGSFRSTSISDGIVRLYSYRALKDYPLAVIVGAAE
                     DEALQEFRGRRDGYLAYAAGMSLLVLFFGGVAIRLLNRQTQISDALDEARVRAESANR
                     MKSEFLASMSHELRTPLNGIIGYAEYLRDTLEGPDKEFAQTIHESGEHLLELVNSILD
                     LARVESGKMTLEFQEEMPRSLIDKVVRAQLPTAEAKGLQLETRFADDLPDHIVCDGTR
                     VVQVLNNLVNNAIKFTDQGQVTVTAGRDGKMMRVAVKDTGCGIPADMQDSIFERFRQV
                     EGFLTRRQQGSGLGLALVKELLTLMGGSVTLESTPGQGSEFIIRIPFTPARPDPLGHA
                     S"
     gene            complement(14850..15497)
                     /locus_tag="PKX01_10610"
     CDS             complement(14850..15497)
                     /locus_tag="PKX01_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HMZ76622.1"
                     /translation="MNNTTGLIGRLRTTGWGLAALIFIAGIVFWGGFNTVLELTNREA
                     FCISCHEMKDNVYQEYRNTIHASNRSGVRATCPDCHVPKDWIPKIIRKIQASNEVLHK
                     VLGSIDTPEKFNAKRLELAKHEWERMKKTDSRECRNCHNYDSFDYAEQGRRSSRMHQT
                     GLNEGKTCIDCHKGIAHQLPQVEQSIGAPKEGATPEEFHPAPAAPAPATAPAEKS"
     gene            complement(15522..16007)
                     /locus_tag="PKX01_10615"
     CDS             complement(15522..16007)
                     /locus_tag="PKX01_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HMZ76623.1"
                     /translation="MNTPKIQTLRLRAAALALCLVHVLGVGILGAPAQAADAPVKLQS
                     LRGAEVPASDPEGDGARYGRDQPPMPRDFVQQPPLIPHTIRGYTITKNFNKCLDCHAW
                     SRAKETGATKISVTHFKDREGQELSSVSPRRYFCSQCHVPQSDAKPLVGNTFRPGVGM
                     R"
     gene            complement(16007..16876)
                     /gene="napH"
                     /locus_tag="PKX01_10620"
     CDS             complement(16007..16876)
                     /gene="napH"
                     /locus_tag="PKX01_10620"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HMZ76624.1"
                     /translation="MATAPSCQLSASPSADRRSLWQRQRWLILRRLSQASILLLFLAG
                     PWFGLWLVKGNLSSSLTLDVLPLTDPLLLLQTLVTGHVPEATAFLGAALVTGFYLLVG
                     GRVFCGWVCPVNIVTDAAAWTRRRLGIKTGRVPDAATRLWLLAGVLGVAAVTGRLAWE
                     WVNPVSLLHRSLIFGLGGGLWMMAAIFLYDVLVASRGWCGHLCPMGAFYGLLGQSALV
                     RVSAHRRKACDDCMDCFAVCPEPQVIRPALKQAGQTHPLILSADCTTCGRCIDVCDKQ
                     VFKMTHRFDQRSE"
     gene            complement(16879..17796)
                     /gene="napG"
                     /locus_tag="PKX01_10625"
     CDS             complement(16879..17796)
                     /gene="napG"
                     /locus_tag="PKX01_10625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HMZ76625.1"
                     /translation="MDPVRNAARPPGRPAESPASAARRKFLNGIAAAAGGGCLLALGA
                     GLYSGSASALPAQALRPPGALAEADFLAACVRCGLCVRDCPYGTLRLADLGEAVPVGT
                     PFYTARRVACEMCEDIPCVKACPTGALDHGLTDINRARMGLAALVDHESCLNFLGLRC
                     DVCYRVCPVIDKAITLETQHNPRSDRHAMLLPTVHSDACTGCGKCEKSCVLPEAAIKV
                     FPRKLAQGALPAHYRKGWEEKARHGGSLIGDQVELPIRGFEGSTAGRAPTQTVVAPTA
                     PAPALPAPTADLPRPRTPAGSGINSHWKP"
     gene            complement(17874..>19208)
                     /locus_tag="PKX01_10630"
     CDS             complement(17874..>19208)
                     /locus_tag="PKX01_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ76626.1"
                     /translation="LLVGKISTPGSGPFSLTGQPSACGTAREVGTFAHRLPADMVVTN
                     PDHRHHTEEIWQLPEGTINEKIGYHAVAQSRALKDGKLKCYWTSTTNNMQAGPNVNQE
                     IYPGWRNPAAFVVVSDVYPTVSALAADLILPSAMWTEKEGAFGNAERRTQFWRQQVKA
                     PGEARSDLWQYMEFSKRFKVEDVWPAELIAKKPEYKGKTLYDVLYANKVVNKFQKAET
                     VKVNEHAIKNYTNDESEAFGFYVQKGLFEEYALFGRGHGHDLALFDVYHKARGLRWPV
                     VDGKETLWRFREGYDPYVKAGEDIKFYGHKDGKAVIFALPYQDPPEKPDADYDLWLCT
                     GRVLEHWHTGTMTRRVPELHKAVPEAQVFMHPDDAKKRGLQRGMQVKVQSRRGEMLAR
                     IETKGRNKPPVGLIFVPFFDESKLVNKLTLDATCPISKETDYKKCAVKVVRA"
BASE COUNT         2945 a         6211 c         6858 g         3194 t
ORIGIN      
        1 ctctccgacc ccctgctggc cggcctcaag cagtcgccgc ggcccgtcag cgggcgctgc
       61 ggggcctgcc gttacctggc catgtgcaat ggcaacaccc gggtccgcgc ctggcagctc
      121 accggcgatc cctggcagga agatcccggc tgctacctgg acaacgccga gatcggcgtg
      181 gccgatggcg agcgtctgga ggtgaagccg tgggtgcgtc tgcagccggc ctgatccgcc
      241 tggcgcaagg gcccatgact tttccgctcc ggtggatggg cttcagcctc tcgcgagcgc
      301 cgttcaccgg cgcgcgggtg accgttcctc gccgacgact gcgccccatt cgcccgcgag
      361 gggccgtcac tcgcagacga atgctcttca cccgcgcgcg agtgattttt actcgcggac
      421 gagtggccaa tacccaccgg cgagtgccgg ccggtcgtcc gcgacccgtg tccattcgcg
      481 cgcgaccggc gttcacgcgc ccgccagtga aattcaccca cgtgcgagga ctgctcactt
      541 gcgcgcgggt gcgcgccctt cgcccgcggg gcggacacga tcaagacccc gtaccggaac
      601 ggccctgggc accgggaggg atggaatgaa aagactgctc gccagcctcg cctttgcggc
      661 gacgctcttg cccaccgttg cggccgccgt ggtcgacgca ccggccctgt acacccagca
      721 ctgcgccagc tgtcacgggc cgggccgcct cggtgccatg ggtccggccc tgctgccgga
      781 gagcctggag cgcctgcgcc gtgctgaagc gatcgccacc cttcgcgacg gccgcaccgc
      841 gacccagatg ccggccttcg gggcgatcct ctcggcggcg gagatcgagg ccctggcgga
      901 ctgggtgcgc acgccggtga gtccggcgcc cgcctggagc gaggacgaca tccgcgcctc
      961 ccgcgtcgag cccttccccc gcggcagcct gccggatgcg ccccagggcc cggtggccca
     1021 gaccgatccg atgaacctgt tcatcgtcgt ggaggccggc gatcaccacg tgagcgtgct
     1081 cgacggcgac cgcatgacgc gcatccaccg ttttccgtcc cgctatgccc tgcatggcgg
     1141 gcccaagttc acgccggacg ggcgctacgt gttcttcgcc tcccgtgacg gctgggtgag
     1201 caagttcgac ctgtggaacc tgaaggtggt ggccgaggtg cgcgccgggc tcaacacccg
     1261 caatgccgcg gtgtcggcgg acgggcgctt cgtggcggtg gcgaactacc tgcccggcaa
     1321 cgtggcgatc ttcgacgcgg acctgaagct gctgcgcctg atcccggcgc gcacgctgaa
     1381 aggcgacgcg agctcccgcg tggcggcggt gtacgacgca ccaccgcggc gcagcttcat
     1441 cgtcggcctc aaggatgtgg cggagatctg ggaagtgtcc tacgacccgg acgccaagcc
     1501 catcttcgat ggctacgtgc acgactacaa gatgggcgag gcgatggccc tgccaggctt
     1561 cctcaattcc cgtcgcacgg cgctggacac gcccctggac gacttcttct tcacccagga
     1621 ctacgccaac gtggtgggcg ccagccgcga aggccgcggc gaggtggtca acctggacgt
     1681 gcgccgcgcc atcgccaccc tggacctgcc cggcatgccc cacctgggct cgggcatctc
     1741 gtggatgcgc gacggccgcc gggtggtggc cagcacccac ctgaaggagg ggcgcgtcag
     1801 tgtgatcgac ctgcagcgct ggcagaccgt ggcgacgatt cccaccctgg ggcccggctt
     1861 cttcctgcgc agccacgaga acagccccta cgcgtgggtc gattcgatga tgagccccac
     1921 cgccaaggac accctgcagg tcatcgacaa gcagcgcctg gcggtggtag cccaagtgcg
     1981 gcccgagccg ggtcggacct tcgcccacgt ggagttcgac cgccacgggc gctacgtcat
     2041 cgccagcctg atggagaacg acggcgcgct gatcttcctc gacgccacca ccttccagga
     2101 gatcaagcgc ctgccggcga gcaagcccat cggcaagtac aacctgttca acaagatcgg
     2161 aaaatcggaa ggcaccagcc actgaggtgc cacccggagc agcgggcgcg gctgcgaccg
     2221 gcggccacgg tagtgggtgc ggtcccgcat cgccgaggcc gcgacggctc gcggggggac
     2281 gatccggcgt ccgcagtccg ccaggcggcg gcggttaaca gccctgggtg aagcgatcgt
     2341 tctggtagcg caggcgcagg gcgcgcagcg tctcggcctg ctggggtggc gtgggctggc
     2401 gcagggcctc gtcgatctcc cggatgtgca gccagagctc ttcacacagg ccggctttct
     2461 ccgggtgatc gggatcgcgc gggatgacca gcggctcggc ggcgccgggc gtcaccctgg
     2521 ggcggttcgc gaccagatcg gcgtattgct cctggatgct gggggtcggt gagccgggcg
     2581 gcggcgcctg gatattcacg cgctcggagc gctcgccggc ggcgcagggg cgatcggaat
     2641 agctgcgctg gccgttggca atgcacaggt aggcgcggct gtccggcgat ggcggggcga
     2701 ccggcgggct ggcgaccggg ggcgcctccg cggacggtgt cggcgcggcc tccgggaggg
     2761 cgggtgccgg gcggttctgc tccgcccaca gccagagcgc ggcggtggcg gcacaggaca
     2821 caagcaggac ggtgccttcc ttcatggcgg tggcggatgg tcggatcggg gcggaactgt
     2881 agcatccctg cgccgggccc ggcggagcgt ccgccggatc ggagcgcctt gacgcggctc
     2941 aatgacccgc gacgcgcccg cgcccaagct gcacgggtcc ttcctgcgag cgcccccatg
     3001 agcgaaaccc ttgcccccgt gatcgtcgat gcccgcggcc tgatgccgcc ccagcccatg
     3061 catctgaccc tggaagccct cgacaccctg cccgccggcg gcgaggtgat cctcttgctc
     3121 taccgggaac cgacgcccct ctacgacgtg ctgatccgca acggctacgt ctatcgcgcc
     3181 gaatccacgc ccggcggcga gttcgccatc cacatccgcc accgcacgga ctgatctgtc
     3241 cgcgtcagtc gtgcgcggcg aggcgttcgg cgtcgtggat gacgatctgc ttgccgtgca
     3301 ccgagatgag accggcgccg gacagctcgt ggaagatgcg cgacagggtt tccggcgtca
     3361 ggctcaggcg tgaggcgatg acctgcttgc tggtgggcag ctcgacgctc cgttcgccct
     3421 gggcggcgca gggcgtgctt tcggccagct gcagcaggta gccgatgacc cgctgggtgg
     3481 aggagcgcag ggcataggat tccacgtccc gcaccatgga gtgcaggcgg atcgacaggc
     3541 cggcgagcag cttgcgggcg aatttcggat cggcgtcgat gaggccgaac acggcggccg
     3601 agccgatatg caggatcaag gcgtcggcca gggcctcggc gagcaccgga tagggctggt
     3661 ctaggaacat cacggcctcg ccgaagctct gcagcggccc gaggatctcc accaccttct
     3721 cgttgccctg ggaagaggta aaggccagct tgacctggcc ccacaccacc aggaagaagc
     3781 cctttggcgc gtcgccgcgc tggaacaggc gttcgccctt gtcgaggcga cgctcgcggg
     3841 tggccgcagc gatgtgggcg agatcgtccg gcgacagggc gctgaacatg ggcatgcggc
     3901 tcaggaggcc gggaatgtcg atcttgtcgg ggctcatcgg gggactccac gttccggcgg
     3961 ggtgccggca ctgcattcta ccccgccggc cctgcaccag gtcggtgcgg catccgcggg
     4021 agcggcgtcc tgcgccgatc tgtgaagtcc tgtgaatgac atgcggaaat cgcggcggtg
     4081 gcgtgaattt tgtcgctgag gcccggcgga tccgtgactt tctgcccgtc gcggggggga
     4141 catccttcct tagcgaagcg ttggccgcgg ggcatcatcg gggcaaccct taacgttcga
     4201 tggacacaac ccatgaagtc cgctgccgat tcccagaccg ggcctggcgc ctcccgagag
     4261 gatgccctgt cgcccgacgc caccctcttc gaggccctgt ccctctttct cgccggggga
     4321 ccgtcgtacc tgccggtggg tgacgccggg ccggcgcgcc gctacctggg ggccgccacc
     4381 ctgctcggcg ccctccatgc cggcctgtcc ggcgacacgc cggtgtcgcg tctgaacctg
     4441 gacacctcgg ccgacagcgc caccgcagcc ggttcggcga agaccctgat cgacgccttg
     4501 cccctgctcc tgtggatgaa ggacgccgaa ggccgctatc tgatcgtcaa tcgcagcttt
     4561 gccgaagcct gcggacggag tacatcggcc gaggtgatcg ggcgggccga cgacgagctg
     4621 tggcccgccg ggcgcgcggc gctgcatgcc gcagccgacg ccgaggtgct ggcgggcggc
     4681 gaggccatgc gggcggtgga ccaggtgagc ggcggagcct cgccggtgtg gctggagagc
     4741 gtgcgggcgc ccctgcgcgg cgctgaaggc ggcatcgtcg gcacggtggg ctttgctgtg
     4801 gatgtgagtg agcgcatccg tctgctggcc gccgccgagc gcagcgagaa ggacctcaac
     4861 ttcgtgctgt cagccatcgg tgaaggcgtg tgggaccgag atctggccag cggcacggtg
     4921 cgtcacaacc ggcgctggtg cgaactgctg ggactcggcc acgcgcagct gatccattcg
     4981 gtggacgacc tgctggcgat gatccacgag gccgaccggc ccctctataa cgccgccgtg
     5041 gccgcctgca tggcccagca cggggcctac atctgcgagt accgcatgcg tcgccccgac
     5101 ggacgggtgc tgtgggtgcg cgatcggggc gacgtggtgg aattcgacgc cgacggccag
     5161 ccctcgcgca tgatgggcag catcgctgac atcaccgaag tgaaggaaag ccagcgtcac
     5221 ctggagcaca tggcctacca cgacaccctc acccagctgc ccaaccgcag cctgttctac
     5281 gatcgcatgc agatggccgt cgcccaggcc gagcgcaatg gcaccctggc ggccatcgca
     5341 tacctggacc tggacggctt caaggcggtg aatgattccc tcggccacgc cgtgggcgac
     5401 cgcctgctgg tcgtcatcgc ccgccgtctg atggcctcca cgcgggccgg cgacaccgtg
     5461 gcgcgcatcg gcggcgacga gttcgcgctg atctacaacg ggctcgagcg cagcgaggag
     5521 tgcgagcaga tcctgcaacg cctgctcgcc agcatcggcg agccggtgac catcgacaac
     5581 cgggagctga ccgtcaccgc cagcgtgggc gtgaccctct gcccgctgga cggctccgat
     5641 ccggaacacc tgctgctgca cgctgacgag gccatgtacc tggccaagga atccggccgc
     5701 aacgccttcc agctctacga tccgggccgc gaccggcgcc tgcgcgccct gcgcgacgcc
     5761 cagcaccggg tcgagctggc cctcgacggg ggcgaactgg agctgcacta ccagcccaag
     5821 gtggatgtgc tcaccaacgc cctcaccggc gtggaggcgc tgatccgttg gaagcaccct
     5881 gagctgggtc tgcgcatgcc cggcgagttc ctgccggcag tgaaggattc ccgtttcgag
     5941 atcgagctgg gcgactgggt gctgcgtacc gcagtgaagc agcttgacgc gtggcaccgg
     6001 gaaggcctga gcacgccaat gagcgtcaat atcgccgccc ggcatctggt gcacccggag
     6061 ttcgtcgcca acctgcgccg tctgctggcg gcctatccgg ccgtgcaccc ggagctgctc
     6121 cagctggagg tgctggaaac ggcgaccctc ggcgacgtga agcgggtggc cggcatcatg
     6181 gcggcgtgca acgaactggg cgtgagcttc gccatcgacg atttcggcac cggccagggc
     6241 gccctgtcca gcctgcgcag gctgccggtg cgcagcatca agatcgaccg ctccttcatc
     6301 cacaacatgc tggaggacga ggaagacctg gccacggtgc agagcgtgat cggcctcggc
     6361 gcagccttcc accgggaggt gatcgccgag ggcgtggaga cgccgggtca ccgtcaggcc
     6421 ctgctgcgcc tgggctgccg catgatccag ggccatggcg tggcccgtcc gatgccggcg
     6481 gcgcgcatgc agcgctggat gcaggaacac cgcacgcagc actgatcccg tcgcccgtgc
     6541 cgcctgccag gcggctccgg cggtccggcc gcagcgtccg ccggacggcc gaccgcccgt
     6601 cacggattca tgcgaccatg cgcgccgcgg ctcccgtgcg ggccgcatcc ggacaactgc
     6661 cgcgagccgc catggaattc ctcaccgacc cgcaattgct gatcgccttt ctgaccctca
     6721 ccgcgctgga gctggtgctg ggcatcgaca acgtcatctt catctccatc ctggtggaca
     6781 agctgccgcc ggaacggcgc gccttcaccc ggcgcctggg gctgttcctg gccatgttca
     6841 tgcgcatcgg cctgctgatg atcctgtcct ggctggcggg gctcaccgaa cccctgctga
     6901 cggtgctggg gcatggcttt tcggggcgtg acctgatcct gctgggaggc ggtctgttcc
     6961 tggtgtggaa gagcacgatg gaggtccacc agctcatgga aggcgaggaa ggcgaggcct
     7021 ccagtaccgt tcaggccacc tttggcgcgg tgctgctgca gatcgtcctg atcgacatcg
     7081 tgttctcgct ggattccatc atcaccgcca tcggcatggt gaacaacctc accgtgatga
     7141 tcgccgcggt gatcgcctcg gtgggcctga tgatggtggc ctccggcccc atcggcgact
     7201 tcgtgtcgcg ccatcccacc gtgaagatgc tggccctgtc ctttctgctg gtgatcggcg
     7261 tggtgctgat tgccgacgcc ttcggccagc acgtgcccaa gggctacatc tactcggcga
     7321 tggccttctc ggtgatggtc gagatgctca acctgcgcat gcgcagccgc caggcggccg
     7381 agccggtgaa gctgcacgaa ccctacgtgc gcgaaccggg ccgcgacgcg tgagcgccgg
     7441 ggacggcgcc gccgcgccgg cgcccttcgg gccgctcacg ccggatttcg tgctggcggc
     7501 ggtggaatcc gccggcctct tctgtgacgg gcgtctgctc acgctcaaca gctacgagaa
     7561 ccgggtctac caggtgggca tcgaggacgc ggcacccctg gtggccaagt tctaccggcc
     7621 ggggcgctgg agcgacgcgg cgatcctgga ggaacacacc ttcctggccg agctggcggc
     7681 ggcggagatc cccgccgtgc cggcgctgga actggacggg cgcaccctgc acgccttcga
     7741 gggctttcgc ttcgcgctgt ttccgcgccg tggcgggcgc gcgccggagc tggacgatcc
     7801 ggccgtgctc acctggctcg ggcgcttcct ggggcgcatc cacgcagtcg gcgcgacccg
     7861 tcccttcgtc caccgaccgg acctgacgcc ccgcagcttc ggcgaggagg cgcgcgacgc
     7921 gctgcttgaa accgccttca ttccggccga gctgcgtgcc acctggcagt cggtggtgga
     7981 tcaggccctg gcgggcgtgc acgcctgtta cgcgcgtgcg ggggaactgc cgacgatccg
     8041 cctgcacggc gactgccacg gtggcaacgt gctgtggacc gacgccggac cgcacttcgt
     8101 ggatttcgac gatgcgcgca acggcccggc gatccaggac ctgtggatgc tcctgtccgg
     8161 cgacgcgccg gccatggcgc ggcagctgga tcacgtgctg gaaggctacg aggccttcgc
     8221 cgacttcgat ccgcgcagcc tgcatctggt ggaggccctg cgcaccctgc gcctcatcca
     8281 ctacgcggcg tggattgccc gccgctggga cgatccggcc tttccggccg ccttcgggtg
     8341 gtttgacagc ccgcgctatt ggcaggacat gatcctggcc ctgcgggagc aggtggcgct
     8401 gatggacgaa tcgccgctga cgcggcagtt ctgacgcggt tccgtgtcga agcggccgtt
     8461 ctggcgcgct tcacgtacgg gcggccgcgc ggcggcaggg ctcaggcggc ggcctgctcc
     8521 gggtattctc cccggcggaa gccgtcaagc atgcgccgca tgcggccggc gtcctcttcg
     8581 gtgaagccgc gcttgaaatc ggtgagcacc aggcggctgc gggtcggcca gttgcccggc
     8641 gcgtcgtcca ccgccagcca gccggcgatg tccgggtggc gcgccagcca ctggcgggct
     8701 tcccgttcgc gcaggccgta gagctcgttg gcttccttgg ggaagatggt gggcgacacg
     8761 tccaccaccc gcgcccgcag atcttcggca aagcgcgccg tcagcttggg cagggagaac
     8821 agcgtgcgcc agtcggacgc gatcaccacg cggacctccg ggtagtcccg caacactgcc
     8881 tcgaacaccg gcagacagcg gaaatcctcg gattcgcccc agggatgggt gaccccgtcg
     8941 aaatccagga acacgaccac cggaccgggg ccggcggctg cgggcggact gacctcggtt
     9001 tgatgcatcg caatatccgt tcaaaaaagg ggtgggactc tgacatctct tgccgataac
     9061 tcaaccatag caagcaatct ggactattct acggtcaggt tttgctgcaa tgcagtattt
     9121 tttcacgcaa gctaggagag tccgtcatgc aaccgattat caatctggca ggcaaagtgg
     9181 gcctgatcac cggcatcgcc aatgagaaat ccatctccac cggcgtcgcg gaagcgtgca
     9241 tcgaggccgg ggcaaaggtg gtcatcacct accagaacga caagaccctg gccttcattg
     9301 agcccatcgt cgcgcgtctg ggtattgagg acgtctacct gctcgacgtg accaagcccg
     9361 agaccatgga cgacgtgttc gcccagatcg acgccaagca cggcaagctg gacttcgcca
     9421 tccactccat ggccttcgcc aagcgtgacg acctgcatgg ccgcgtcgtg gacacctcgc
     9481 cggacggctt cgccctggcc atggacgtgt ccacccactc cttcgtgcgc ctggcccgca
     9541 aggccgagcc cctgttcgag aaggccggcg gcggttcgct gatcaccatg acctacctgg
     9601 gcagcgaaaa ggtcatcgcc aattacggcg tgatgggcct gtgcaaggct gcgctggaag
     9661 ccgcaacgcg ctacatggcc ttcgagctgg gccccaagaa catccgcgtc aacgccatct
     9721 ccccgggccc gctgatgacc cgtgcggcgt ccgggattgc cggctttgac gggctgatgg
     9781 ccgccgcagt cgagcgttcg ccgctgcacc gtctggtgac gccggaggac atcggcgctc
     9841 tcaccgcctt cctggtctcg gacgcgggtc gcaacctgag cggcggtgtg catttcgtgg
     9901 atgccggcta caacatcatc gcctgagccc gccgatggct gtccgacctg atcgcgatgc
     9961 ggccagctac gaccgccgca tcgccgatcc ggtgcccgac caccccctgc tcgccgggct
    10021 ggccgagctc gggcgggggg agtcgtccat cgtgctggac gccggcgacg gggagcgggt
    10081 cttcaaggtg gtcagttccc cggccgacta ttgtttcctc gcggccgatg atcgcccgat
    10141 cggcctgcac ttcccgcgcc tgatcaagga tcacggcgtg gtgggcaagg cgagcaacgg
    10201 ctattccttc cgcctgctcg aactggagcg tctgctgccg gtggcgggcg aggccgaggc
    10261 gctcgggcag ctgctctccg atgcctattg gcagggctgc gagaagtggg ccaacctggg
    10321 tgtcaatcgg ggccgtctgg cgctttacca tatcgcccag gaaccgccgc cgggtctgcc
    10381 gacgagcctg gtcaaggcgg tggcggacct gtcggatttc atcgaaggct atccggtgca
    10441 gccggacatc ctcaatcccc acaacctgat gatgcgtccc gacgggacgc tggtgttctc
    10501 ggatccggtg ttcctgccct gagccggcgt gcggccttca tcccggccgg ttccggggct
    10561 aagatgaggg cggagacatc cctcacgcac aacaacaggc ccccgccatg cttccgacgc
    10621 cattcgaacg gatcaaggat ctcatcggca agatccacgc cctggaggac aagctcatga
    10681 ccacgggcat cctgcccctg agcgaacagg caaagatcca cgagcagatc cgccactaca
    10741 aggaagagat cgccctcctc gagcggcgca cgcgcaagca ttcctgaagc gcgaaacgcc
    10801 ctgagggtgc ccgcccgggg cgtcgtgccg cacgctgcgg cctgcatggg cgtgtgcggt
    10861 ggtttgcgcg gtcacatccc tgtcctagaa tgccgcccgc cgggccggcg tccgccggcc
    10921 catcccattg atttcaggag ttgcacatgt cgggcgatca ttttcacgtc catggccccc
    10981 acgaccacgc ggtcgagcat gccgcggaac atggcggggg cgaccccttc gcgtcgcgca
    11041 tcgcggcgat gacggcgatc ctcgccaccc tgggtgcgct gttcagctat gccggtggcg
    11101 ccacccagaa cgacgcgctg atgcacaaga acgaggcggc catccgcaag acggaggcgt
    11161 ccaaccggtg gggctattac caggccaaga gcgccaagca gaatctggcg gagctgggcg
    11221 tgagccttac gacgggggaa atccagaccc gctacacggc cgagatcgag cgctacaagc
    11281 aggagaagca ggagatcaag agcgcggcgg aggaactgga aaaagccgcc atcgaggccg
    11341 aggaagccag cgaacatgcc atgcacgccc atcaccgctg ggcccaggcc atgacggcga
    11401 tgcaggtggc gatctccctg gcggcgatca cgctgctgac gcggcggcgc tggatgcaat
    11461 ggacctcggt cggggtggcg acggccggat gcgccatcgc cgggctggcg ctggccggca
    11521 tctgaggccg gcgcggcgcg atggcggacg gcttcgcggc gtccgccgtg gccgtcagaa
    11581 cgcgtccttc tgctcgccgc cgagggcctc cagcagggcc gggatgaggc gcgagagttc
    11641 gccggtcatc agggtgaaat ccgcttccat ctgcaggtcg gcggtttcgg cctgacgttc
    11701 ggcctcttcc ttgaccacgt cgaggaaggc gatgcgcttg atctccagct tttcggtgag
    11761 gagcagggcg atgcggtcgt cgaaggtgag ggcgaggcgg gtgggcagct tgccggcggc
    11821 caggtggtcc tgcacgtcct ggccgtcgag ggcgtggcgc acgtagcgca cggcggcctt
    11881 ctcgtcggtg acggcgcgca gttcgcaatc ctggtcgatg gtgaaggcgc cgggcgcctc
    11941 gccgccggcc agccagccgg tcatggcgct gacaggcgac tgctgggtct tgagcagctt
    12001 caccggcagg gcgtcgaggg tgtcacgcag ggcgtcgagg acgtcctcgg cgcgggccgg
    12061 gctggcggca tccacggcga gccagccgcc cttcgggtcg atccacgccc acatcgtgcg
    12121 ccgccgcgtg aaggcgcgag ggaggagttc ctgcagcacc gcctccttga tctccttcat
    12181 ctgcttgcga ccgggcttgt agccctgttc ggcttcgatc agctcggcct tgtcggccgc
    12241 cacctggttg accaccgagg acggcagcag cttctgctcc acggccaggg cgatcaggta
    12301 gtggccgccc acggcgagga cgaagcggtc gtcgcgggtc ggcgaggtcc acccgcggct
    12361 cgccatgtcc tggctgccac agggctggaa ggggcgggcg gcgagcttgt cggcgagggt
    12421 gtcggcgctg aggtcccagt tttcgggcag gcggtagagc tggaggttgc ggaaccacat
    12481 gggcaaatcg atccgtactg agtcgaaggg agcggcattg tagcggcagc cgcagccgcc
    12541 gcagggggga aatcggcctg ctcaggcggg caggcgcagg gcggccagaa ggacctggcg
    12601 gttgatgggc ttgatgagga tgtcgtcgaa acccgcggag aggatgtctt cccgttcgcc
    12661 aggcatggcg tgggcggtgt aggcgatgat gcgtacgccg ccgaattcag ggcggctgcg
    12721 cagctgtcgg cacagttcgg tgccgctgat gccgggcatg ctcacgtcca gcaggatggc
    12781 ctggaaaccg tggctgtcga tgagccggag ggcgtcttcg ccggattcgg cttcgaggac
    12841 ctcgcacccc tgctggcgca ggagcacggc gggcagggtg cgattgacgc gttggtcgtc
    12901 gacgacgagg actttcatga ggcatgtcct agaggatcgg ggcgggcggg cgtgaaggga
    12961 atgcggatga tgaattcgct gccctggcca ggggtggatt cgagggtgac gctgccgccc
    13021 atgagggtga ggagttcctt gaccagggcc agaccgaggc cgctgccctg ctgacggcgg
    13081 gtgaggaagc cttcgacctg acggaagcgc tcgaagatgg agtcctgcat gtcggcggga
    13141 atgccgcaac cggtgtcctt gacggcgacc cgcatcatct tgccgtcgcg cccggcggtg
    13201 acggtgacct gcccctggtc ggtgaatttg atggcgttgt tgaccagatt gttgaggacc
    13261 tgcaccacgc gggtcccgtc gcacacgatg tggtcgggca gatcgtcagc gaatcgggtc
    13321 tcgagctgga gtcccttggc ctcggcggtg ggcaactggg cccggacgac cttgtcgatg
    13381 aggctgcgcg gcatctcctc ctggaattcg agggtcatct tgccggattc gacgcgggcc
    13441 agatcgagga tggaattgac cagttcgagc aggtgttcgc cgctctcgtg gatggtctgg
    13501 gcgaattcct tgtccgggcc ttcgagggtg tcacgcaggt attcggcgta gccgatgatg
    13561 ccgttgaggg gcgtgcgcag ctcgtgggac atggaggcga ggaactcgga cttcatgcgg
    13621 ttggcggatt cggcgcggac acgggcctcg tcgagggcat cgctgatctg cgtctgacgg
    13681 ttgaggagcc ggatcgccac gccgccgaag aacagcacca gcaggctcat gccggcggcg
    13741 taggcgaggt agccgtcgcg gcggccgcgg aattcctgca gggcctcgtc ttcggccgcg
    13801 ccgacgatga cggccagggg atagtccttg agggcccggt agctgtacag gcgcacgatg
    13861 ccgtcggaga tgctggtgga acggaagctc cccgtcgtgc ccgtgttggt ctgggtgaag
    13921 aaggggctgg cgctgatgtc ctggccgacg gaatcgttgt tgccggagcg gcgggcgcgc
    13981 acgataccgt ccaggcccac gagggcgact gcgccgtggg tgcccaggtc cacgcccttg
    14041 tagaaatcgg agaagtacat cggatcgagg gagatcacca ccacgccgcc gaagctgccg
    14101 tcgggcttgt tgatccgccg cgtcatctgg atcgaccact tcttggaggc gcggcccagc
    14161 accggcttgc tgatgaagag ctggttggtg tcccgctcca tgtggacctt gaagtgttcc
    14221 cggtcggaga ggtcgatctt cttgtgattg gggaggttgc tgaggatgta gatcccgtgt
    14281 tcgtcgatga cccccagctg gttgaagatg gtgctgatga tcatgccttc ccgcacgtac
    14341 tcgccgatgt cgaccttgtc accgtacttc tcgtactgga acttgaggaa gagcaccgcc
    14401 tgatcgacgc tcttgagggt gcggatggtg tgttcctcaa aggcacgggc gaggttgagg
    14461 ttttccacgt tgaccgagtg gatgaccagt tcctcttcgg tatggatctt gtacaggatc
    14521 gccccccaga tcagcacgat cagggcgatg accagggcga cgatggggcc gtgcaggcgt
    14581 cggctggaag gagaaccggg tggattgggg gacgacatgg gctaccttgg ctgcgggcgc
    14641 gaaagacccg tcgccctgac gggtttctat aacgtcccgg tgacgccggg cttgagggct
    14701 gcgtgtccgg ccgtggcgca gatagtacga cgtcttgggg gcggccgcat accgatggcg
    14761 tggccataaa gacgacgggc tgccacagca gcccgtcgtc tcccctgtgc tccggccggg
    14821 ggcgaccgga gcctgctcca ccgaatgcgt cagctctttt ccgcgggggc cgtcgccggc
    14881 gcgggtgcgg ccggggcggg gtggaattcc tcgggcgtgg caccctcctt gggcgcgccg
    14941 atagactgtt ccacctgggg aagctggtgg gcgatgccct tgtggcagtc gatgcaggtc
    15001 ttcccttcgt tgaggccggt ctggtgcatc cgcgacgagc gacgaccctg ctcggcatag
    15061 tcgaagctgt cgtagttgtg gcagttgcgg cactcgcggg aatccgtctt cttcatccgc
    15121 tcccactcat gcttggccag ctccaggcgc ttggcgttga acttctcggg tgtgtcgatg
    15181 gagccgagca ccttgtgcag gacttcgttg gaggcctgga tcttgcggat gatcttgggt
    15241 atccagtcct tgggcacgtg gcaatccggg caggtggccc gtacgccgga gcggttggac
    15301 gcgtgaatgg tgttgcggta ttcctgatac acgttgtcct tcatctcgtg gcaggagatg
    15361 cagaaggctt cccggttggt cagttcgagg acggtgttga agccgcccca gaacacgatg
    15421 ccggcgatga agatcagcgc ggcaagaccc cagccggtgg tgcgcaggcg gccgatgagg
    15481 ccggtcgtat tgttcatgtc agggctcccg gtgatgatgg atcagcgcat gcccacgccc
    15541 gggcggaagg tgttgcccac caggggcttg gcatcgctct ggggcacatg acactggctg
    15601 cagaagtaac ggcgcggcga cacgctggac agctcctggc cctcccggtc cttgaagtgg
    15661 gtcaccgaga tcttggtggc gccggtctcc ttggcacggc tccaggcatg gcagtccagg
    15721 cacttgttga agttcttggt gatcgtgtag ccgcggatgg tgtgcgggat caggggcggc
    15781 tgctggacga agtcgcgggg catgggcggt tggtcgcggc cgtagcgggc accgtctcct
    15841 tccgggtcgg atgccggcac ttcggcgccg cgcaggctct gcagcttgac cggggcgtcg
    15901 gcggcctggg caggagcgcc gaggatgccc acgcccagga cgtggacgag gcacagggcc
    15961 agggcagcgg cccgcaggcg gagggtttgg atttttggcg tgttcatcac tcgctccttt
    16021 ggtcgaaccg gtgggtcatc ttgaatacct gtttgtcgca gacgtcgatg cagcgtccgc
    16081 aggtggtgca gtccgcggac aggatgaggg ggtgggtctg gccggcctgc ttgagggccg
    16141 ggcggatcac ctggggttcc gggcacacgg cgaagcagtc catgcagtcg tcgcaggcct
    16201 tgcgccgatg ggccgagacg cgcaccaggg cactctggcc gagcaggccg tagaaggcgc
    16261 ccatcggaca caggtggccg caccagccgc ggctggcgac cagcacgtca taaaggaaga
    16321 tggcggccat catccacagg ccgccgccga gtccgaagat caggctgcgg tgcagcaggg
    16381 acacgggatt gacccactcc caggcgaggc ggccggttac cgcggccacc cccagcacac
    16441 cggctagcag ccacaggcgg gtggcggcat cgggcacgcg gccggtcttg atgccaagcc
    16501 ggcggcgcgt ccacgcggcg gcgtcggtga cgatattgac cgggcacacc cagccgcaga
    16561 acacccgccc gcccaccagc aggtagaagc cggtgaccag ggcggcgccg aggaaggcgg
    16621 tggcttccgg cacgtggccg gtgacgaggg tctggagcaa cagcagtgga tcggtgaggg
    16681 gcagtacgtc cagggtgagg gaggaggaca ggttgccctt gacgagccac aggccaaacc
    16741 acggcccggc caggaagagg agcaggatcg acgcctgact gaggcgtcgc aggatgagcc
    16801 agcgctggcg ctgccacagg ctgcgccggt cggcggaggg gctcgcggaa agctggcagg
    16861 acggggcggt ggccatgctc agggtttcca gtgggagttg atgccgctgc ccgccggggt
    16921 gcgtgggcgg ggaaggtccg cggtcggcgc cggcagcgcg ggggccgggg cggtgggtgc
    16981 gacgaccgtc tgggtcggcg cgcggccggc agtggagcct tcgaagccgc ggatcggcag
    17041 ttccacctgg tcgccgatca gggagccgcc gtggcgggcc ttttcctccc agcccttgcg
    17101 gtagtgggca ggcagcgcgc cctgggcgag cttacgcggg aagaccttga tggccgcttc
    17161 gggcaggacg caggatttct cgcacttgcc gcagccggtg caggcgtccg aatggaccgt
    17221 cggcagcagc atggcgtgac ggtcggagcg cgggttgtgc tgggtttcga gggtgatggc
    17281 cttgtcgatg acggggcaca cccggtagca cacgtcgcag cgtagaccga ggaagttcag
    17341 gcagctctcg tgatccacga gggcggcgag gcccatgcgg gcccggttga tgtcggtgag
    17401 gccgtggtcg agggcgccgg tggggcaggc cttgacgcag gggatgtcct cgcacatctc
    17461 gcaggcgacg cggcgggcgg tgtagaaggg cgtgcccacc ggtaccgcct cgcccagatc
    17521 ggcgagacgc agggtgccgt aggggcagtc gcgcacgcac aggccgcagc gcacgcaggc
    17581 ggccaggaaa tcggcctccg ccagggcgcc gggcgggcgc agggcctggg ctggcagggc
    17641 cgacgcgctg ccggaataga gcccggcccc gagggcgagc aggcagccgc cgcccgcagc
    17701 ggcggcgatg ccgttgagga atttgcgtcg cgctgcggag gccggcgatt cagccggcct
    17761 gccaggcgga cgggcagcgt tgcggacggg gtccatgatg attccgatgt cggatcaggc
    17821 ggccggcgtg cgtgggcaag ggggcgtcac gcggccggtc gccgtagcca gcctcaggcg
    17881 cgcaccacct tgacggcgca cttcttgtag tcggtttcct tcgagatcgg gcaggtggcg
    17941 tcgagggtga gcttgttcac cagcttggat tcgtcgaaga agggcacgaa gatcaggccc
    18001 accggcggct tgttgcggcc cttggtctcg atgcgggcga gcatctcgcc gcggcgggac
    18061 tggaccttga cctgcatgcc ccgctgcaga ccgcgcttct tggcgtcgtc cgggtgcatg
    18121 aagacctggg cttcgggcac ggccttgtgg agttcgggca cgcggcgggt catggtgccg
    18181 gtgtgccagt gctccagcac gcggccggtg cacagccaca ggtcgtagtc ggcatcgggc
    18241 ttttcgggcg gatcctggta gggcagggcg aagatcacgg ccttgccgtc cttgtggccg
    18301 tagaacttga tgtcctcgcc ggccttgacg taggggtcgt agccttcacg gaagcgccac
    18361 agggtctcct tgccgtccac caccggccag cgcaggccgc gggccttgtg ataaacgtcg
    18421 aagagggcca ggtcgtggcc gtggccgcgg ccgaagaggg cgtattcctc gaacaggccc
    18481 ttctgcacgt agaagccgaa ggcttcggat tcgtcgttgg tgtagttctt gatggcgtgc
    18541 tcattgacct tgacggtctc ggccttctgg aacttgttca ccaccttgtt ggcgtagagc
    18601 acgtcataca gggtcttgcc cttgtattcg ggcttcttgg cgatgagctc ggcgggccac
    18661 acgtcctcca ccttgaagcg cttggagaat tccatgtact gccacagatc ggagcgggcc
    18721 tcgccaggcg ccttgacctg ctgccgccag aactgggtgc gccgctcggc gttgccgaag
    18781 gcgccttcct tctcggtcca catggcggag ggcaggatca ggtcggcggc cagggcggag
    18841 acggtgggat acacgtcgga caccaccaca aaggcggccg ggttgcgcca gccggggtag
    18901 atctcctggt tgacgttggg gcccgcctgc atgttgttgg tggtgctggt ccagtagcac
    18961 ttgagcttgc cgtccttgag ggcgcggctc tgggcgacgg cgtggtagcc gatcttctcg
    19021 ttgatggtgc cttccggcag ttgccagatt tcctcggtgt ggtgacggtg atccgggttg
    19081 gtgaccacca tgtcggcggg caggcggtgg gcgaaggtgc ctacttcccg cgcggtgccg
    19141 caggcggagg gttgaccggt gagggagaag gggccggagc ccggggtgga gatcttgccc
    19201 accagcag
//
LOCUS       DAOQPA010000069        18842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1467_length_18842_cov_18.832437, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000069 DAOQPA010000000
VERSION     DAOQPA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_18842_cov_18.832437"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            740..1348
                     /locus_tag="PKX01_10635"
     CDS             740..1348
                     /locus_tag="PKX01_10635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ76627.1"
                     /translation="MDEHLIVLDKPSGLLSVPGRGLERADCLASRVHVRFADARIVHR
                     LDMATSGLIVMARGAHMERALSIAFQQRQVEKCYEALVAGRPAQVEGDIHLPLITDWP
                     RRPRQKVDPDTGKPSHTHFRVLAYDPGADVSRVALIPHTGRSHQLRVHLQAIGHPILG
                     DELYAPDAAFQAAPRLMLHACRLCFAHPALGHRLELNSPPPF"
     gene            complement(1361..2305)
                     /locus_tag="PKX01_10640"
     CDS             complement(1361..2305)
                     /locus_tag="PKX01_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76628.1"
                     /translation="MTSSALTPRDVLTPEALAMIQRIAELGSFAAAARATGQVPSALS
                     YRVRQIEEALDVLLFDRSARQARLTEAGGELLREGARLLGEIDAIANRVKRVATGWEP
                     RLTIAVDSIIHRTTVLELCEAFFALGAPTRLRLRQETLSGTLETLGSGEADLALGVAV
                     ESISVAGVQGRPLGSIRFLFAVAPHHPLARAPEPLSDELIGRHRAVAVADTVRVGSGQ
                     TVGLLGGQDVFTVPNLEAKLEAQLRGIGAGFLPECLVRSYADSGRLVTKAVQRSERVV
                     HLGYAWRAAGSGVPRKALQWWLERLEHAATRAALLGVR"
     gene            2417..3115
                     /locus_tag="PKX01_10645"
     CDS             2417..3115
                     /locus_tag="PKX01_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ76629.1"
                     /translation="MLALRRARDRGFADHGWLQSHHTFSFAGYHDPAHMGWGNLRVIN
                     EDRIAPGTGFGAHSHRDMEIVSYVLNGALAHRDSLGNGTVIQPGDVQRMTAGTGVTHS
                     EFNDAAETTHFLQIWLLPARRNLPPGYAQRTFPEADKRGRLARIAAPDGADGAVLLHA
                     DACVYAGLLDGAEQFTLDLDPSRKAFVHLIRGRLRVNGQMLEPGDGAALADENRLHLD
                     AAEDAEVLVFDLAP"
     gene            3156..3710
                     /locus_tag="PKX01_10650"
     CDS             3156..3710
                     /locus_tag="PKX01_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMZ76630.1"
                     /translation="MSKIAVVFHSGYGHTLRLAQSVAVGAGAELVPIDAEGNLPEGGW
                     DTLNAADAIIFGSPTYMGTVSWQFKKFADASSKAWFTQAWKDKVFAGFTNSATMNGDK
                     LSTLHYLFTLAMQHSGVWVGTGLMPSNSKAAQRDDVNYVGSFCGAMAQTPSDASPDEI
                     PAGDLETARLFGQRVAAVTARFGR"
     gene            complement(4475..4551)
                     /locus_tag="PKX01_10655"
     tRNA            complement(4475..4551)
                     /locus_tag="PKX01_10655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4515..4517),aa:Pro,seq:cgg)
     gene            complement(4649..5617)
                     /locus_tag="PKX01_10660"
     CDS             complement(4649..5617)
                     /locus_tag="PKX01_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ76631.1"
                     /translation="MSLSQLYAPIADDMKALDAVIRERLYSDVVLIRQVAEYIIGSGG
                     KRLRPALVLFSAGAMGYRGRFHLELAAVVEFIHTATLLHDDVVDESDLRRGNKTANAL
                     FGNAASVLVGDFLYSRAFQMMVGVDDMRVMRVLADATNTIAEGEVLQLLNCHNADVDV
                     EDYLRVIRYKTAKLFEAATRLGGILGKADPQLEEQLAAFGMHLGTAFQLVDDVLDYSG
                     DEAATGKHLGDDLAEGKPTLPLIHAMRHGSPEQAARVRAAIENGGRDDFAGVLDAVRA
                     TGALEATLAVATQEAERALAMLEGLPPSIFKDSLVHLSKFAVAREY"
     gene            complement(5697..6425)
                     /locus_tag="PKX01_10665"
     CDS             complement(5697..6425)
                     /locus_tag="PKX01_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HMZ76632.1"
                     /translation="MVAHRCGGALAPENSLAGLEIAARLGCSAVEFDVMLSRDTVPVL
                     MHDETLERTAGLPGRVPDLEGGELAAVDIGRLHHPAFAGECIPRLDTALERCRWLGLA
                     ANVEIKPADDLDEVTARAVIAVVTQCAERDRPAILFSSFSRKALEQVALALPAIPRAL
                     LAEHYDADILACARALGCGAVHLEATSLRASDVAAVHAAGLWLRAYTVNQVDLACRLV
                     EWGVDGLFSDRPDRLLRLDALSAG"
     gene            complement(6455..7762)
                     /locus_tag="PKX01_10670"
     CDS             complement(6455..7762)
                     /locus_tag="PKX01_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMZ76633.1"
                     /translation="MKNVCRVAAGGAVLGLALLSPVYAAPAKKVAPAVPQATAPVGDL
                     ELVHTLGPDKGAQLQKLVDRFNAANPGTHIVVSERPWTEGALPHMLILGERDLPAFLA
                     GPQRYRPLYQVMKDAGERVETLKAPAFMTPTPLDASNRLLALPVGLSTPVMFYNRDAF
                     KAAGVNPDEPPKTWQDLQGALGKLVTNGSTCPYTSAQPAWILVENSSAWHNEPVVAEG
                     KRASLAINGMLPVKHVAMLSSWFKARYLHVFGRGDEATEKFAQGECAVLTAPSAALPT
                     LRREAKFDVGVATLPYHAEYYGAPQNTLADGPALWIGAGKKPAEYKAIARFVNLWLTP
                     ESQVEWQRNAGYLPLNRAGLLAATESRVLGPELEAIRIGVKQLTHKPVTKISSASSYP
                     QRTAVRQIVEEELEAVWADRKPSKVAMDDAVARSRSLGCSPSC"
     gene            7912..8367
                     /locus_tag="PKX01_10675"
     CDS             7912..8367
                     /locus_tag="PKX01_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76634.1"
                     /translation="MKITSVRLALMLALGPTLLGGCERLGIPDPAKAQAQAEAEGKAI
                     GSACRHAGRAIEDCYTLNQAAPKAAIFSGWKEMNDYMVENKIAEVAPQLPPPPPPAPP
                     QKTGKKAKDGKGDKDEDHGDKEDAADSDKSSGGHRADEETPRRRRRSAE"
     gene            complement(8398..10185)
                     /locus_tag="PKX01_10680"
     CDS             complement(8398..10185)
                     /locus_tag="PKX01_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HMZ76635.1"
                     /translation="MNSIVLRERNGIAVIGNEALARMLARYGFGQAQQAAIRAFGRIV
                     DGQGLTESLLERLDLLSPDGRGEGAIGPALRLFCEDLACLPQWDFTPRWPERLVASWS
                     ECYLAGAVAEFPFVAIQQLVDLCQQRLFDEREMVYRLEMDIFSAVIRLCWCLGGLLSE
                     VSIEREQAFRLYAEDGDPVLGIPNRRRFLALLDQQLAGPDPVGLVVIGVDWGRSEALL
                     PMDERDGIRLAFTEALQGTVRPADILCALGEKEWAVILPGIHHPAQVSLAGHKMVDAC
                     EILRNNSFVSLNGRFAAGGALAPEHATDPLGLEQAARAALVAARHANGYFDLYSASLA
                     RASRADADMEDEVVRAFEACQFDLYLQPQICLTDAQVIGAEALLRWPRGEGVVVGPQR
                     ILAVLGNVGMIQALSRWVIQRAVQAVAALRAAGCQIRVSVNLSAEDLRDQELAVFVRQ
                     TCETWRVPAERLCFEITEGGFVADEAMTARTLESLRETGGWLSLDDFGTGYASMDYLR
                     RMPVQELKLDKSFVERLNRDEADRAIVELMIRIAHTFGLEVVGEGVEDADTALTLAAL
                     GCDHAQGYHYARPMPVDDFIDWWQTREGG"
     gene            complement(10182..11711)
                     /locus_tag="PKX01_10685"
     CDS             complement(10182..11711)
                     /locus_tag="PKX01_10685"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HMZ76636.1"
                     /translation="MKEIVAVQDLAVGSFICELDRPWLESPFLIQGFLVADETILAQV
                     RSLCRFVYVDRARSVGVEWRPDTVTPAGDADVRAARLAEFHVPAADARRRPQDFFALL
                     RAMRERKADDANADERTREPFVYRYPDRPERARSGSVAEPSADASPAGRVSRPIVTPT
                     GDGKRTGARRAAGGEEGASPSGAAANAGLVYGGPAVEEEIVAAAPLVDEAQVILKEIA
                     RDVQSNLNPDLERLRAVVGEMVLSVARNPDALLWLVRLKQTDQYSYDHSLDVAAHVMI
                     YARHLALPEDVIATLGIAGMLQDVGKLRMPVRLLQKVGAITRTEYEIFKTHVDFSVQI
                     LAANPNASPLLLEIVGQHHERCDGSGYPRGLKDEQISLAAQIAGLCDVYCAMTRNRPY
                     GKAASSQAALDELRAMRGRGFAETTVDQFVQCIGIYPVGTLVELNSGEVAVVIAQNRV
                     RRLKPRVMLLLGPDKKPNAYPLVVDLLYDPPNLAGDPYAIVRSLPPGAYGVDPSEYYL
                     A"
     gene            complement(11757..12794)
                     /locus_tag="PKX01_10690"
     CDS             complement(11757..12794)
                     /locus_tag="PKX01_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ76637.1"
                     /translation="MEVRQLGGSDLRVSAVCLGTMTFGQQNSEAESHALLDLAMARGV
                     NFIDTAEMYPVPPRAETCGRSESIIGTWLRGRPREQVVIATKVAGPARSMDWIRGGPL
                     SLDRDNIRVAVEGSLRRLQTDYIDLYQIHWPARNQPMFGQWRFDAAAEREATPIREQL
                     EAMAELVREGKIRYVGLSNEHPWGVMEFLRHARELGLPRVVSVQNAYNLLNRNFEYTL
                     AETCFREQIGLMPYSVLAFGHLTGKYLVDPPAQGRLSLFPAFGQRYGKANVQPAVQAY
                     AALAQEAGLSPATLAQAFVAGRPFVASTIVGATSPAQLEENLAACARVLEPAVVAQVD
                     ALHLQFTNPAP"
     gene            12951..14039
                     /gene="rlmM"
                     /locus_tag="PKX01_10695"
     CDS             12951..14039
                     /gene="rlmM"
                     /locus_tag="PKX01_10695"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HMZ76638.1"
                     /translation="MSTPSPHCLTLGALIQCRPGFEPDAAAELASRAHAARLVGPVSG
                     APRSGFCTLTLAEPLPRDLLQRRLDASQLVFSRQSFQVVAVLDDLPERDRLTPLVSAI
                     AAGGERFCRLLLETPDTNEGKETSGFLRRFEPLLDAALRDVGLLRPDSPHLPRLHVFF
                     ANARHVWLGFSAPSDGSAWPMGIPRLRMPKDAPSRSTLKLAEAFLTLLTPDEQKATLR
                     PGLQAVDLGAAPGGWSFQLASHGLRVTALDNGPLAPAALATGLIEHLRADGFTWKPRK
                     PVEWMVCDMVEQPARIAPLMAEWLASGRCRRSLFNLKLPMKRRYEELERCRALIDQRL
                     RHAGIKYVLRIKHLYHDREEVTCYLTRR"
     gene            14115..15566
                     /locus_tag="PKX01_10700"
     CDS             14115..15566
                     /locus_tag="PKX01_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ76639.1"
                     /translation="MSFSELGLIPEILQAVADAGYTEPTPIQRQAIPVVLAGHDVMGG
                     AQTGTGKTAGFTLPLLNRLARHASASASPARHPVRALILAPTRELAMQVYESVRMYSK
                     HLPLRSVCIYGGVDMNPQIQELRRGIEIVVATPGRLLDHVQQKTIVLNQVEFLVLDEA
                     DRMLDMGFIPDIRRILALLPAQRQSLLFSATFSDEIKKLADQMLKNPQLVEVARRNMV
                     SETITHVVHPVSSGLKRNLLAHLIRHEEEAQVLVFVDTKFGCSRLAHFLERHGISADA
                     IHGDKSQQARTETLENFKSGKTRVLVATDVAARGLDIEDLPSVINFELPHTAEDYVHR
                     IGRTGRAGKTGKAVSLVSAEERHRLADIQKLIKVEIRQEVVAGYDPEPDFFEADGKRR
                     RHAASAPKAEAGGEPRERHERRERRPERSPRESSGRGGPRTPHIAPDGFDFSKPYEQR
                     ETPPHTLADAADASPKKARKPIAFLLGGLGRRD"
     gene            complement(15663..15893)
                     /locus_tag="PKX01_10705"
     CDS             complement(15663..15893)
                     /locus_tag="PKX01_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HMZ76640.1"
                     /translation="MVFDKEVDARGLNCPLPILRAKKALAELQAGQVLRVLATDPGSA
                     KDFAAFARQTGHELIEQNERPDQSFEFFLRRK"
     gene            16261..16614
                     /locus_tag="PKX01_10710"
     CDS             16261..16614
                     /locus_tag="PKX01_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HMZ76641.1"
                     /translation="MLQTVLSDLNGTSADIEASAIISSDGLMMAALLPNTVDEDRVGA
                     MSAAMLSLGDRTARELARGNLEQVLIKGEKGYVLMLQAGADAVLTVMAKPSAKLGLVF
                     LDVKRAADSIARILG"
     gene            16634..17107
                     /locus_tag="PKX01_10715"
     CDS             16634..17107
                     /locus_tag="PKX01_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ76642.1"
                     /translation="MTDMDPASIVGNARECTLQHFQSVARKVYVTDVETPYPQGKLIV
                     SRTNVEGVITHCNQSFVDMSGYEEHELVGQPHCILRHPDMPAAAFRDLWETVQAGRQW
                     HGYVKNLRKDGGYYWVYATVVPNVRDGQIVGYTSVRREPARNRIAEAEALYRSMS"
     gene            17110..17856
                     /locus_tag="PKX01_10720"
     CDS             17110..17856
                     /locus_tag="PKX01_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HMZ76643.1"
                     /translation="MTLNFTVSPSFPNERISDWYIFNTWLQRALSAQVHLELYDGFGA
                     QRQAIADGKVDLIYANPFDASLLVREKGFRAVAGPDDRADEMVVFTTDASSARQIADL
                     KPGTRLACTDDPAVNTVGRILLEPAGLGPDNTVTVQKETYVQVARDVLQGHVDVGFMP
                     VEAFEGLSRLVRRDLRPLVTSQLHALFHVFLVGPALAARSDEIAELLTGLNDSDKGRQ
                     MLAALGSTHWRRVSQEDAEFMIDIISTLAQ"
     gene            17853..18329
                     /locus_tag="PKX01_10725"
     CDS             17853..18329
                     /locus_tag="PKX01_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76644.1"
                     /translation="MSLFITDTAQPGRIRPPSVPAVFGLPVRTLNIASHWHSPATGAL
                     LAAEIGRTPANLHAHVQRISLWSSLGDSRELIAAIIDLWMVLGPHGMDLRKRMLDTHR
                     EILEREGLAAWMSAQLSTGADRHDPLLARPGVVLARPVRGIRRFLRTPATLSAART"
     gene            18425..>18842
                     /locus_tag="PKX01_10730"
     CDS             18425..>18842
                     /locus_tag="PKX01_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76645.1"
                     /translation="MKHPPRPSARSQPLALHLLEVGSMDQRLIGLSVERHMKHVLRLV
                     TEPDQARAVLIDCDRQGAQDALRQARLLRGVGIVGYAFNPRERNNAFPGIEILNKPLA
                     LDRLPEALDRAIAAAGAAPPTIPADSPPPAAPPPPPP"
BASE COUNT         3479 a         6695 c         5668 g         3000 t
ORIGIN      
        1 ccgagcgctt gagtttcaaa ccatgacctg tccacgggat gggggcaact tcagggcatc
       61 ctcaacggcg cggactggca gaagcccaag gggatgcact ggcgaacatt cacccgcctg
      121 cacgcggaac atgatcggct tgtgagtgcg tcgcttgccg ggatcatggg gcggcttacg
      181 ggtgccttgc ccgcggcact ggattttgag gattaacccc atgcaaggcc ccgcggacga
      241 cacagcaggc ccgccgcgca ggataattgg cgtagcttca cgcatcgcgc gcgcgaggtg
      301 cgagaatccc ttaaaatctc gggcagctca cgcaagatgg gggcaacttt gggggcaact
      361 gtcaaacagc acgcagtaaa tcccttgtaa aacgtggctt tcaagcaaca gttcgaaagc
      421 ccccagcgaa tcgaactggc ggcgatcctg gaggctggca tcgcggtagt gatcgagtca
      481 agctgctacg attcacccag gaagaggggc ggatccgcac cgttccgcag cggtttgcag
      541 cggccgtaag gcgctgagtt atcttggttt ggcgcgggtt acaggcccac caccacccga
      601 catgcgcgag accgtcactg cagtgacggt ctcgtttttt caacgcttca atcatctgat
      661 cggtatggcc gcggaatcca ccgcaacgcc gcccgcgccc ccttacctgc cgcctcgagg
      721 cgaacccatc gtcgtcttca tggacgagca tctcatcgtg ctcgacaaac cttcgggact
      781 gctgtcggtg ccgggacgcg ggctcgagcg tgcggattgc ctggcgagcc gggtacacgt
      841 ccggtttgcc gatgcacgca tcgtccatcg gctagacatg gccacctccg ggctgatcgt
      901 catggccagg ggtgcccaca tggaacgcgc cctcagcatc gcgttccagc aacggcaggt
      961 ggagaaatgt tacgaagcgc tcgtggcagg acgccctgcc caggtcgaag gggacatcca
     1021 cctgcccttg atcaccgact ggccccggcg accccgccag aaggtggatc ctgacacggg
     1081 gaagcccagt cacacccact tccgcgtgct cgcttacgat ccgggcgctg acgtgagccg
     1141 cgtggccctc atcccgcaca cggggcgctc ccaccagttg cgcgtccatc tacaggcaat
     1201 cggccacccg atcctcggcg acgaactcta cgctcccgat gccgccttcc aggcggcccc
     1261 ccggctgatg ctccacgcat gccgcctgtg tttcgcccat ccagccctcg ggcaccggct
     1321 ggagctgaac agcccgcccc ccttctgacg gactgcgtcc tcaacgcacg cccagcagcg
     1381 ccgcccgggt cgccgcgtgc tcgaggcgct ccagccacca ttgcagcgcc ttgcgcggca
     1441 ctccggaccc ggctgctcgc catgcgtagc caagatgtac cacgcgttcg ctgcgctgga
     1501 ctgccttggt caccagacgg ccgctgtcag cgtaggaccg gaccaggcat tccggcagga
     1561 atcccgctcc gataccgcgc aattgagctt ccagcttggc ctccaggttc ggcacggtga
     1621 acacgtcctg cccgcccagc agtcccacag tctgaccgct gccgacacgt acggtgtccg
     1681 caacggccac ggcacgatgg cggccaatga gctcatcgct gaggggctcg ggtgcgcggg
     1741 cgagtgggtg gtggggtgcc accgcgaaca ggaagcgtat ggatcccagc ggacgtccct
     1801 gtacgcccgc caccgagatc gactccaccg ctacaccgag ggccagatcg gcctcgcccg
     1861 agcccagcgt ctccagtgtg ccggagaggg tctcctgacg aagccgcagg cgcgtcggtg
     1921 cgcccagggc aaagaaggcc tcgcacagct ccagcacggt cgtccggtga atgatgctgt
     1981 ccaccgcgat ggtgaggcgg ggttcccaac cggtggccac ccgtttgacc cgattggcga
     2041 tggcatcgat ctcacccagc agccgggcgc cctcccgtaa cagttcgcct ccagcctcgg
     2101 tcagtcgggc ctggcgcgcg ctgcggtcga agagcagcac atccagcgcc tcttcgatct
     2161 ggcgcacgcg atagctcaag gcgctcggca cctgtccggt cgcccgcgcc gcggcggcga
     2221 aactgcccag ttcggcgatg cgctggatca tcgccaatgc ctcgggcgtc aggacgtccc
     2281 ggggagtcag tgcactggaa gtcatagtaa aaacacaaat cattcgaata atttgaatga
     2341 tgccatcaaa cgccgtggct catgcgcccc gatgaccccg gtaaagtgac ttcacccaga
     2401 caccaggagg cccatcatgc tcgcactccg ccgcgcccga gaccggggtt tcgcagacca
     2461 tggctggctg caaagccacc acaccttctc ctttgccggt taccacgacc ctgctcacat
     2521 gggctggggc aacctgcgtg tcatcaatga ggaccgcatc gcgccgggta ccggctttgg
     2581 cgcccacagc caccgggaca tggagatcgt gagctacgta ctcaacggcg ccctcgccca
     2641 tcgcgacagt ctgggcaatg gcacggtgat ccagcccggc gacgtacagc gcatgacggc
     2701 aggcaccggg gtcacccata gcgaattcaa cgatgctgcg gagaccaccc atttcttgca
     2761 gatctggctg cttcccgccc ggcgcaatct gccgcctggg tacgcgcaac gtacttttcc
     2821 agaggccgac aaacgcggcc ggctggcgcg gattgcggcg cccgacggtg cagacggtgc
     2881 cgtcctcctg catgccgacg cctgcgtcta tgcggggctg ctggacggcg ctgagcagtt
     2941 cacgctggac ctggatccgt cccgcaaggc ctttgtccat ctgatccgcg gccgcttgcg
     3001 agtcaatggc cagatgctgg aacccggcga cggcgccgcg ctcgccgatg aaaaccggct
     3061 gcatctggac gccgccgagg acgccgaagt gctggtcttc gatctggcac cctgatcgcc
     3121 ccacaacgac tttaccccca cccctggaga accacatgag caagatcgcc gtcgtctttc
     3181 attccggcta tggccacacc ctgcgcctcg cccagtccgt cgccgtcgga gccggcgccg
     3241 agctcgttcc catcgatgcc gaaggcaacc tgccggaagg cggctgggac acattgaacg
     3301 cagctgacgc catcatcttt ggcagcccga cctacatggg caccgtgagc tggcaattca
     3361 agaaatttgc cgacgccagc tccaaggcct ggttcacgca ggcatggaag gacaaggtct
     3421 ttgccggctt caccaacagt gccaccatga atggcgacaa gctgtctact ctgcactacc
     3481 tgtttaccct ggccatgcag catagcggcg tgtgggtcgg caccggcctg atgccgtcca
     3541 acagcaaggc cgcgcagcgg gacgacgtga actacgtggg ctccttctgc ggcgccatgg
     3601 cccagacccc ctccgacgcc agccccgacg aaattccggc tggggacctg gagacggcac
     3661 gcctgttcgg ccagcgcgtc gctgcagtga ctgcccgttt cggtcgctga tccacctctg
     3721 cggacattgc ggcgcatcgc ccgccgcaat gtccctccaa cggacggaaa tcggttagac
     3781 caagtcacgg cacccatcgg tacgaggcat ccgccccgcc ttgccgggaa tcctcacgcg
     3841 cacaccctcg gcggcaggac gaaggtcggc ccgcatcgca gatcgaccgt tggcccaacc
     3901 ccacgtgaca aatccgccca ggaaggtcgc aggcgccgag gcggagcaat ggtgcatctc
     3961 ccggagcatc acccaggtcc gccgtgccag acgctcgccg ccaggttgcg catcgttgtg
     4021 atcgtccgga atgcggtcgg taccaaaccc acgccgtacg tcagggtgat ggcaggcagc
     4081 ctgaacagcc ttgaataggc ggtacgggga cgtcccttca accacgtttg cactgacgtc
     4141 aggtttcgtc agctcgcgca cccgattctg agtaactcgc cgttgttccc ctgagttaga
     4201 cagccgtggc agcgcgcccc tgactcgaca cgataccgcg ccattcatcg caaacgaact
     4261 ggaatcgagc acccactcac cgcggtatct gtgcccgcca acccgcctcc agccgctcac
     4321 gattcgtgcg tcttccggtg caggacatcg cgcgagacag gacaacggtt gaggcccaga
     4381 gatccgacgt cggcatgatg catgcctcga aaacctaaat ccggaaggcg tagcacgcaa
     4441 aaaagccact ccgcagagtg gctttttaaa gtgatggtcg gggaaagagg attcgaacct
     4501 ccggcccctg cgtcccgaac gcagtgctct accaggctga gctattcccc gacacgatcg
     4561 aaagatggcg aaaacacaaa tgccaccttt ctgaaacaaa acagccgcac tgactatcga
     4621 cgattcggac tgtgctgcac gccgaacatc aatattcccg cgcgactgcg aactttgaca
     4681 agtgtaccag cgaatccttg aaaatggaag ggggcagccc ttccagcatc gccagcgcgc
     4741 gctcggcctc ctgggttgcg accgcaagcg tagcctccag tgcgccggtg gcacgcacag
     4801 cgtccagcac gccggcaaag tcgtcacggc ccccgttctc aatggcggcc cgcaccctcg
     4861 ccgcctgctc gggtgaacca tgccgcatgg catgaatcag gggcagggtc ggcttgccct
     4921 ctgcaagatc gtcacccaga tgcttgcccg tggccgcctc gtcgccggag taatcgagca
     4981 catcgtccac gagctgaaag gcggtgccca agtgcatgcc aaaggccgca agctgttctt
     5041 ccagttgcgg atcggccttg cccaggatgc cccccaggcg cgtcgctgcc tcgaacagct
     5101 tggcggtctt gtagcggatc acccgcaggt agtcctccac gtccacatcg gcgttgtggc
     5161 aattgaggag ctgcaagacc tcaccttcgg cgatcgtgtt ggtggcatcg gccagcacgc
     5221 gcatgacgcg catgtcgtcc acacccacca tcatctggaa cgcacgcgaa tacaggaaat
     5281 ctcccacaag caccgacgca gcgtttccga acaatgcatt ggccgtcttg ttgccccgcc
     5341 gcagatccga ttcgtcgacc acgtcgtcat ggagcagcgt ggcggtgtgg atgaattcca
     5401 ccacggcggc gagctccaga tggaaacgcc cgcgataccc catggcaccg gccgaaaaga
     5461 gcacgagcgc ggggcgcagg cgcttgccgc cgctgccgat gatgtattcg gccacctggc
     5521 gaatcagaac cacgtcggag tagagacgtt cgcggatgac ggcatcgagc gccttcatgt
     5581 cgtcggcgat cggtgcgtaa agctgcgaga gagacaaggc agtgcccaga cgggaaaaag
     5641 cgcgatggta gggggagctc agcccgtgcg tcaagcggcg tggcgtaata ccctgctcag
     5701 cccgctgaca atgcgtccag ccgcagcagg cggtccggtc ggtcactgaa cagaccatcc
     5761 acgccccact ctacgaggcg acacgccaga tcgacctgat tgaccgtgta cgcccgtagc
     5821 cagagccccg ccgcgtgaac ggctgcaaca tcgctggccc gcagtgacgt cgcctcgaga
     5881 tgcacggcac cacagcctaa ggcgcgggcg cacgccagaa tgtcggcgtc gtagtgttcc
     5941 gccaggaggg ctcggggtat cgcagggagt gcaagggcca cctgttccag cgccttgcgc
     6001 gagaaagacg agaaaaggat cgccggtcga tctcgttccg cacactgcgt caccacggca
     6061 atcaccgccc gtgcggtcac ctcgtccaaa tcgtcggcgg gtttgatctc cacattcgca
     6121 gccagaccca gccaccggca gcgttcaagc gccgtatcga ggcgcggaat gcactcccct
     6181 gcgaaggcag ggtgatgcag gcgaccgata tcgactgcag cgagctcccc cccttccaga
     6241 tcgggaacac gcccggggag cccggcggta cgctccaggg tttcgtcgtg catgagcacc
     6301 ggcacggtgt cccgtgacag catgacgtcg aactccaccg cggagcaacc gagccgcgcg
     6361 gcaatctcca gcccggcaag ggagttctcg ggcgccagcg caccgccgca gcggtgggcc
     6421 accacccggg gccacgctcc gccgggtccc tccatcaaca ggagggcgag cagccgaggg
     6481 aacgcgaacg ggccacggca tcgtccatgg ccaccttgga gggcttgcgg tcagcccaca
     6541 cggcttccag ttcctcttcg acgatctggc gcacggcggt acgctgggga tagctgctcg
     6601 ccgacgagat cttggtgacc ggcttgtggg tcagctgctt gacaccgatg cgaatggcct
     6661 ccagttcggg cccgagcaca cggctttctg tcgccgccag cagaccggca cggttcagcg
     6721 gcagatagcc cgcgttgcgc tgccattcga cctgactttc aggcgtcagc cacagattga
     6781 cgaaacgggc gatggccttg tattcggccg gcttcttgcc agcaccaatc cacagagccg
     6841 ggccgtccgc cagcgtgttc tggggtgcgc catagtattc ggcgtgatac ggcagcgtgg
     6901 ccacacccac gtcgaacttc gcctcgcgac gcagggtcgg gagggcggcg gacggcgccg
     6961 tgaggacggc gcattcacct tgggcaaact tctcggtggc ttcgtcgccg cgaccgaaga
     7021 cgtgcaggta gcgggccttg aaccaggagg acagcatggc cacatgcttg acgggcagca
     7081 tgccattgat ggccagggaa gcccgcttgc cctccgccac caccggctcg ttgtgccagg
     7141 cgctgctgtt ttccaccagg atccaggcgg gctgggccga ggtgtaggga caggtgctgc
     7201 cgttggtaac aagcttgccg agcgcgcctt gcaggtcctg ccaggtcttg ggcggctcat
     7261 ccggattgac gcccgctgcc ttgaaggcat cacggttgta gaacatcacc ggcgtggaca
     7321 ggccgaccgg cagggccagc agtcgattcg aggcatccag cggggtcggc gtcatgaagg
     7381 ccggcgcctt gagggtttcg acccgttcgc cagcatcctt catgacctgg tagaggggtc
     7441 gatagcgctg cggcccagcc aggaaggcag gcaggtcccg ttcgccaaga atcagcatgt
     7501 ggggcagcgc gccttccgtc cagggccgct cgctgacgac gatatgcgta cccggattgg
     7561 cagcgttgaa gcggtcgacg agcttttgta gctgggcacc cttgtcgggt ccgagggtat
     7621 gcacgagttc gagatcgccg acaggcgcgg tggcctgggg cacagccggg gccaccttct
     7681 tggctggcgc cgcgtaaacg ggggacagta gggccaggcc caggacagcg ccgccggcgg
     7741 cgacgcgaca gacattcttc atggagaggg aatccgcagg aaacaaggag agaggttacg
     7801 caccgctaac cggtcattat agggacaagc cgcggcggga agtttaagca aatgttcaag
     7861 tcacctgcag accggccgtt gactggcttg cctcacgatc cctcgacgca gatgaagatc
     7921 acctctgtcc gccttgcact catgctggcc ctcggcccca cgctgctggg ggggtgcgag
     7981 cgcctgggca tccccgatcc ggccaaggcc caggcccagg cggaagccga aggcaaggca
     8041 attggcagcg cctgccgcca tgccgggcgg gccatcgagg actgctacac cctcaaccag
     8101 gcggccccca aggcggcaat cttctccggc tggaaagaga tgaacgacta catggtcgag
     8161 aacaagatcg ctgaagtcgc gccccagctg cctccgccgc ccccgccggc gcccccgcaa
     8221 aaaaccggca aaaaagcaaa ggacggcaag ggtgacaagg acgaggacca cggcgacaag
     8281 gaggacgccg ccgattcgga caagagcagc ggcgggcatc gcgccgacga agaaacgccc
     8341 cgcaggcgcc gccgcagcgc cgaatagcac cgcagttccg tccgggcatc gcccggttca
     8401 acccccttcg cgcgtctgcc accagtcgat gaaatcgtcg accggcatcg gccgtgcata
     8461 gtggtacccc tgcgcgtgat cgcaccccag cgccgccagt gtcagcgcgg tgtcagcatc
     8521 ctcgaccccc tcgcccacta cttcgaggcc gaaggtatgg gcgatgcgga tcatcagttc
     8581 gacaatggcc cgatcggctt cgtcccggtt gagtcgctcc acaaaggact tgtccagctt
     8641 gagctcctgg accggcatac ggcgcaggta atccatcgag gcgtagccgg tgccgaaatc
     8701 atcgagcgac aaccagccgc cggtttcgcg caggctctcc agtgtccgtg ccgtcatcgc
     8761 ctcatcggcc acaaagccac cctcggtgat ctcgaagcac aggcgctccg ccggcacgcg
     8821 ccaggtctcg caggtctgcc gcacaaagac ggccaactcc tgatcgcgca ggtcctctgc
     8881 ggacagattc accgacacgc ggatctggca tcccgccgcg cgcagcgcag cgaccgcctg
     8941 caccgcacgc tggatcaccc agcgcgacag ggcctggatc atccccacat tgccgagcac
     9001 tgccagaata cgttgcggcc cgaccacgac cccctctccc cgcggccagc gcaacagggc
     9061 ttccgcgccg atcacctgtg cgtccgtcag gcagatctgg ggctgcagat acaggtcgaa
     9121 ctggcaggct tcaaacgcac ggacgacctc gtcttccatg tcggcatcgg cccgcgaagc
     9181 ccgcgcgagg gacgccgaat agaggtcgaa atagccattc gcatgacggg ccgccaccag
     9241 cgcggcgcgg gccgcctgct ccagcccgag cgggtcggtc gcatgttccg gcgccagcgc
     9301 cccgccggcg gcaaagcgtc cattcaggga gacgaagctg ttgttgcgga ggatttcaca
     9361 ggcatccacc atcttgtgtc cggccaggga gacctgcgcc ggatgatgga tgcccggcag
     9421 gatcacggcc cattccttct caccgagcgc gcacagaatg tcggcgggcc gcacggtccc
     9481 ctgcagggcc tcggtaaacg ccagacggat accgtcgcgt tcgtccatgg gcagcagggc
     9541 ctccgaacgt ccccaatcga cgccgatcac caccagacca acgggatcgg gcccggcgag
     9601 ctgctgatcg aggagcgcca ggaaacgacg ccgattggga ataccgagaa ccggatcgcc
     9661 atcctccgcg tacaggcgga aggcctgctc ccgctcgatg gacacctccg acagcaatcc
     9721 ccccaggcac cagcacaggc ggatcacggc gctgaagatg tccatctcca gccgatagac
     9781 catctcgcgc tcatcgaaca ggcgctgctg gcacagatcg acgagctgct ggatggcgac
     9841 aaacgggaac tcggcaaccg ctccggccag gtaacactcc gaccagctcg ccaccagccg
     9901 ctccggccag cgcggcgtga aatcccattg gggcagacac gccagatcct cgcagaacag
     9961 gcgcaacgcg ggaccgatgg cgccttcgcc ccggccgtcc ggactcagca ggtcgagacg
    10021 ctccagcaag gactcggtca atccctgccc atccacgatc cgcccaaagg cccggatcgc
    10081 tgcctgctgg gcctgtccaa agccatagcg cgccagcatg cgtgccaggg cctcgttgcc
    10141 gatgacggcg ataccgttgc gctcccgcag tacgatgctg ttcatgccag gtaatactcg
    10201 gaaggatcga ccccataggc gccgggcggc agggatcgca cgatggcgta aggatcaccc
    10261 gccagattgg gcgggtcgta gagcagatcg accaccagcg gataggcatt gggcttcttg
    10321 tccggcccga gcagtagcat cacccggggc ttgagccggc gcacgcgatt ctgggcgatc
    10381 accaccgcca cctcgccgga gttcagctcg accagcgtcc caaccgggta gatgccgatg
    10441 cactggacga actggtcgac cgtggtctcc gcaaagccgc gcccacgcat ggcgcgcaat
    10501 tcgtcgaggg ccgcctgcga gctggccgcc ttgccgtagg gccggttccg cgtcatggcg
    10561 cagtagacgt cgcacaggcc cgcgatctgg gccgcgagac tgatctgctc gtccttgagc
    10621 ccgcgcggat agcccgaccc gtcgcagcgc tcgtggtgct gcccgacaat ctccagaagc
    10681 agcggcgacg cgttcgggtt ggccgccagg atctggaccg aaaaatccac gtgggttttg
    10741 aagatctcgt actcggtgcg ggtgatcgca cccactttct gcagcaggcg caccggcatg
    10801 cgcagcttgc ccacgtcctg gagcatgccc gcaatgccga gcgtggcgat gacgtcctcc
    10861 ggcagggcca gatggcgcgc gtagatcatc acgtgcgctg ccacgtcgag ggagtggtcg
    10921 tagctgtact gatcggtctg cttgagccgc accaaccaca ggagcgcgtc cggattgcgg
    10981 gccacgctca gcaccatctc gccgacgacg gcccgcaggc gctccagatc ggggttgaga
    11041 ttgctctgca cgtcgcgggc gatctccttg agaatcacct gcgcctcatc caccagcggc
    11101 gcagccgcga cgatctcctc ctccacggcg ggcccgccat agacgaggcc agcgttggcc
    11161 gccgcacccg acggcgacgc gccctcttca cccccggccg cgcgccgggc gccagtacgt
    11221 ttgccatcgc cggtcggcgt cacaatcggt cggcttaccc gaccagcagg gcttgcatcc
    11281 gccgaaggct cggccacgga accgctgcgc gcgcgctcgg gacggtcggg ataacggtaa
    11341 acaaagggct cacgggttcg ctcgtccgcg tttgcgtcgt ccgccttgcg ttcacgcatg
    11401 gcgcgcagca gcgcaaagaa atcctgggga cgccgacggg catccgctgc cggcacatga
    11461 aactctgcca gccgcgcagc gcggacgtcg gcgtcacccg ccggcgtgac cgtgtcggga
    11521 cgccattcca cgcccaccga acgggcccgg tccacataga cgaagcgaca cagggagcgc
    11581 acctgcgcaa ggatcgtttc gtcagcgacg agaaatccct ggatcaggaa cggcgactcc
    11641 agccatggcc ggtcgagttc gcagatgaac gaaccgaccg ccagatcctg aaccgccacg
    11701 atttccttca aacccttctc ccgatgacca caacaagccg cgtcgcaaga gacgtactac
    11761 ggtgccggat tcgtaaactg aagatgcagc gcgtccacct gcgccacgac ggccggttcc
    11821 agcacccggg cgcacgcggc cagattctct tccagctggg caggcgaggt cgctccaaca
    11881 atcgtgctgg cgacaaacgg ccgcccggcc acaaaggcct gagccagcgt ggccggtgaa
    11941 agaccggctt cctgcgccag cgccgcgtag gcctgcaccg ccggctggac attggccttg
    12001 ccgtagcgct gaccaaaggc cgggaagagc gacaggcgcc cctgcgcggg cggatccacc
    12061 agatattttc cggtgagatg cccgaaagcc agcaccgaat acggcatcag gccgatctgc
    12121 tcgcgaaagc aggtctctgc cagcgtgtat tcaaaattgc ggttgagcaa gttgtacgca
    12181 ttctggaccg acaccaccct aggcaggccc agttcccgcg catggcgcag gaattccatc
    12241 acgccccagg gatgttcgtt ggaaagcccc acgtagcgga tcttgccctc gcggaccagc
    12301 tcggccatcg cctccagttg ctcgcggatc ggcgttgcct cccgctcggc ggcagcgtcg
    12361 aagcgccact gcccgaacat gggctggttc cgcgccggcc agtggatctg gtagaggtcg
    12421 atgtaatccg tctgcaggcg ccgcaggctg ccttccaccg ccacccggat gttgtcccgg
    12481 tcgagggaca gcggcccgcc gcggatccag tccatgctgc gtgccggacc ggccaccttg
    12541 gtggcgatca ccacctgctc gcgcggacgt ccgcgcaacc aggtgccgat gatggattcc
    12601 gaacggccac aggtttcggc ccgcggcggc accggataca tctcggccgt atcgatgaag
    12661 ttcacgcccc gcgccatcgc cagatccagc agggcgtggc tctcggcctc gctgttctgc
    12721 tggccaaagg tcatggtgcc gaggcagacc gccgacaccc gcagatcgct gccacccagt
    12781 tgtcgtactt ccatgcgctt gctccacaga ccggccgggc ggaagccgga cgcaatcggg
    12841 tatgatcggc accggcctgc gcctgcctcg accgtcgagc cccgcccgcc acagaccaac
    12901 cggcgcattc tacatcccgt cccccgccgc ccggcgaccg attatccccg atgagcaccc
    12961 cgtctccgca ctgcctgacc ctgggcgccc tgatccagtg ccgccccggt ttcgagccgg
    13021 acgccgcggc cgaacttgcg tcccgcgccc atgcagcgcg cctcgtcgga ccggtgtccg
    13081 gcgccccccg cagcggcttc tgcaccctga ccctcgccga gcccctgccc cgggatctcc
    13141 tgcaacgccg tctcgacgcc tcccagctgg tgttctcgcg ccagagcttc caggtcgtcg
    13201 cggtgctcga cgatctcccc gaacgggatc gcctgacgcc gctggtgtcc gccatcgcgg
    13261 ccggcggcga acgcttctgc cgcctcctgc tggaaacccc ggacaccaac gagggcaagg
    13321 aaacctcggg cttcctccgg cgcttcgaac cgctcctcga cgcggcgctc cgtgacgtgg
    13381 gcctgctgcg ccccgactcg ccccacctgc cgcgcctcca cgtcttcttt gcgaacgccc
    13441 gccacgtctg gctggggttc agcgcgccga gcgacggctc cgcctggccc atgggcattc
    13501 cgcgcctgcg catgccgaag gacgcgccga gccgctccac cctcaagctc gccgaagcct
    13561 tcctcaccct gctcacaccg gacgaacaga aagccaccct gcgaccgggc ctgcaggcag
    13621 tggacctggg cgccgcgccc ggcggctgga gcttccagct cgccagccat ggtctgcgcg
    13681 tcaccgccct ggacaacggc cccctcgcgc cggccgccct cgccaccggg ctcatcgagc
    13741 acctgcgcgc cgacggcttc acctggaagc cgcgcaaacc cgtcgaatgg atggtctgcg
    13801 acatggtcga gcaacccgcc cgcatcgcac cgctgatggc cgaatggctg gccagcggac
    13861 gctgccggcg cagcctcttc aacctcaagc tcccgatgaa gcggcgttac gaggagctgg
    13921 agcgctgccg cgccctcatc gaccagcgcc tgcgccacgc gggcatcaaa tacgtcttgc
    13981 gaatcaaaca cctttaccac gaccgggaag aggtcacctg ctatctgaca cggcgctgaa
    14041 gtgccgacca tgtataattc aggtttcctg atttggtgca ctgcaaaaac actgcacccc
    14101 aacgcgatcg atccatgtcg ttttccgaac tcgggctgat tcccgaaatt ctccaggcgg
    14161 tggccgatgc cggctacacc gaacctaccc cgatccagcg ccaggccatc ccggtcgtcc
    14221 ttgccggtca cgacgtcatg ggcggagccc agaccggcac cggcaagacc gcgggcttca
    14281 cgctgcccct cctgaaccgc ctcgcccgcc acgccagcgc ctcggcgagc ccggcacgcc
    14341 atccggtgcg cgcactgatc ctcgcaccca cgcgcgaact ggccatgcag gtgtacgaat
    14401 ccgtgcggat gtactccaag cacctgcccc tgcgcagcgt gtgcatctat ggcggcgtgg
    14461 acatgaaccc gcagatccag gagctgcgcc gcggcatcga gatcgtcgtc gccacgcccg
    14521 gtcgcctgct cgaccacgtg cagcagaaga ccatcgtcct caaccaggtg gaattcctgg
    14581 tgctcgacga ggccgaccgg atgctcgaca tgggcttcat cccggacatc cgccgcatcc
    14641 tcgcgctgct cccggcccag cgccagagcc tgctgttctc ggccaccttt tccgacgaga
    14701 tcaagaagct cgccgaccag atgctcaaga acccgcagct ggtcgaggtc gcccgccgca
    14761 acatggtcag cgagaccatc acccacgtgg tgcacccggt ttccagcggc ctcaagcgca
    14821 acctgctcgc ccatctgatc cgccacgaag aagaagccca ggtgctggtc ttcgtggaca
    14881 ccaagttcgg ctgcagccgc ctggcccact tcctggaacg tcacggcatc tccgccgacg
    14941 ccatccacgg cgacaagagc cagcaggccc gcaccgagac cctcgagaac ttcaagtccg
    15001 gcaagacccg cgtactggtc gccaccgacg tcgccgcccg cggcctcgac atcgaggacc
    15061 tgccctcggt catcaacttc gaactgcccc acaccgccga agactacgtc caccgcatcg
    15121 gccgtaccgg ccgtgccggc aagacgggca aggccgtgtc gctggtcagc gcggaggagc
    15181 gtcatcgtct cgccgacatc cagaagctca tcaaggtgga gatccgccag gaagtcgtcg
    15241 ccggttacga ccccgaaccc gatttcttcg aggccgacgg caagcgccgc cgccacgccg
    15301 cctccgcacc caaggccgag gccggcggcg aaccccgcga acgccacgaa cgtcgcgagc
    15361 gtcgcccgga acggagcccg cgggaatcct ctggccgcgg cggtccccgg acgccccaca
    15421 tcgcgccgga cggtttcgac ttcagcaagc cctatgagca gcgtgagacg ccgccccaca
    15481 cgcttgccga cgccgccgat gccagtccca agaaggctcg caagccgatc gccttcctgc
    15541 ttggcggcct cggccggcgc gactgacgca ggcatctccc acggcgtcca aaccagcgcc
    15601 attgcaaaga aaaggccacg cgatgcgtgg ccttttcaca tgccgcccaa tccgcagccg
    15661 cgtcacttgc ggcgcagaaa gaactcgaaa ctctggtccg gccgctcgtt ctgctcgatc
    15721 agttcgtgcc ccgtctgtcg tgcgaatgcc gcaaaatcct ttgccgagcc cggatccgtc
    15781 gcgagcacgc gcagcacctg ccccgcctgc aattcggcca gagccttctt ggcccgcagg
    15841 atcggcagcg ggcaattcaa accgcgggca tccacttcct tgtcgaacac catctcgaat
    15901 cctcttcacc gcagatgaca ggaggcgatt ctaacgtgca caccccacgc ccgaccgccg
    15961 gaatgccccc attcgagggg cgattttggc gcgctttcgc acacgtttgg caaactttgc
    16021 gtttatatca aaatggcaaa ttctgacttt tgccatctgt gaattacgtc accaattcaa
    16081 tcagaatagc gaagtcgggg atatctgact tcatggcata atcgtgctgc caaaatttca
    16141 tatgtcattt tcggatgacc ccgatgtctt gtccgaaaag ggcttttgcc catttcgggc
    16201 agcacccgaa gacattgccc cctcagtcac ttcaagaaag ccaaaaagat gcgctctaac
    16261 atgctccaga cggtcctcag cgacctgaac ggcacctccg ccgacatcga agcctccgcc
    16321 atcatctcct ccgacggcct catgatggcc gccctgctgc ccaacacggt cgatgaggac
    16381 cgcgtgggcg caatgagcgc cgccatgctc tccctgggcg accgcaccgc tcgcgagctg
    16441 gcccgcggca acctcgaaca ggtgctgatc aagggcgaaa aaggctacgt cctgatgctc
    16501 caggccggcg ccgacgcagt gctcaccgtg atggccaagc cctccgccaa gctcgggctg
    16561 gtgttcctcg acgtcaagcg cgcagccgac tcgatcgccc gcatcctggg ttgaaagcga
    16621 ggagtcgagc atcatgactg acatggaccc cgcgtccatc gtcgggaacg cccgggagtg
    16681 cacgctgcag cacttccaga gcgtcgcccg caaggtgtac gtgaccgatg tggaaacgcc
    16741 ctacccccag ggcaaactga tcgtctcccg aaccaacgtg gagggcgtca tcacccactg
    16801 caaccagtcc ttcgtggaca tgtcgggcta cgaggagcac gaactcgtcg gacagcccca
    16861 ttgcatcctg cgtcatcccg acatgcccgc tgcagccttc cgtgatctgt gggaaacggt
    16921 gcaggcgggc cgccagtggc atggttatgt caagaacctg cgcaaggatg gcggctacta
    16981 ctgggtctat gccaccgttg tgcccaatgt ccgtgacggc cagatcgtcg gctacacctc
    17041 cgtccgccgc gaacccgccc gtaacaggat cgccgaagcc gaagccctct accggagcat
    17101 gagctgacca tgaccctcaa tttcacggtc agtccgagct ttcccaacga gcgcatctcg
    17161 gactggtaca tcttcaacac ctggctgcag cgtgccctgt ccgcgcaagt ccacctggaa
    17221 ctctacgacg gcttcggcgc ccagcgtcag gccatcgccg acggcaaggt ggacctcatc
    17281 tacgccaacc cgtttgatgc gtcgctgctg gtgcgcgaga agggattccg cgccgtcgca
    17341 ggtcccgatg accgcgccga cgagatggtg gtcttcacca ccgacgcatc gtccgcacgc
    17401 cagatcgccg acctgaagcc gggcacccgc ctggcctgca ccgacgaccc ggcggtcaat
    17461 accgtcggac gcatcctgct cgagccggcc ggtctgggtc ccgacaacac ggtcaccgtg
    17521 cagaaggaga cctacgtcca ggtcgcccgc gatgtcctgc agggccatgt ggatgtgggc
    17581 ttcatgcccg tcgaagcctt cgaaggcctc tcccgtctag tacggcgcga cctgcgtccg
    17641 ctggtcacga gccagttgca cgcgctcttt cacgtgttcc tggtcggtcc ggccctggcg
    17701 gcacgcagcg acgagatcgc cgagctcctg accggactca acgactccga caagggtcgc
    17761 cagatgctcg ccgcgctcgg cagcacgcac tggcggcggg tttcccagga agatgccgaa
    17821 ttcatgatcg acatcatctc gaccctcgcc cagtgagcct gttcatcacc gacacggcgc
    17881 agcccggacg gatccgtccc ccctccgtcc cggcggtctt cggactgccg gtacggaccc
    17941 tgaacatcgc ttcccattgg cacagcccgg ccaccggcgc gctgctcgcc gccgagatcg
    18001 gccgcacacc ggccaatctg cacgcccacg tccagcgcat ctccctgtgg agcagcctgg
    18061 gcgactcgcg ggaactgatc gccgcgatca tcgatctgtg gatggtgctc ggcccccatg
    18121 gaatggacct gcgcaagcgg atgctcgaca cccatcggga gatcctcgag cgcgaagggc
    18181 tcgccgcgtg gatgagtgcc cagctcagca ccggcgccga ccgccacgat cccctgctgg
    18241 cccgcccggg cgttgtgctg gcgcgccccg tccgcggcat ccggcgattt ctgcgaactc
    18301 ccgccactct gtctgctgcc cggacatgag cagcaatgaa accccggcag gcatgcttca
    18361 gctcatccgg cacgccctct cgtttcccgg cccgtcctcg ccctcctcgc cgacgccaca
    18421 cgccatgaaa caccccccgc gcccttccgc ccgcagccag cccctggccc tgcatctcct
    18481 cgaagtaggc agcatggacc aacggctcat cggtctgtcg gtcgaacggc acatgaaaca
    18541 tgtccttcgc ctggtgaccg aacccgacca ggcccgggcc gtcctcatcg attgcgaccg
    18601 tcagggcgcg caggacgccc tgcgccaggc acggctcctg cgcggcgtgg ggatcgtggg
    18661 ttatgccttc aacccgcggg agcgcaacaa cgcctttccg ggcatcgaga tcctcaacaa
    18721 gccgctggcc ctggaccgcc tgcccgaagc actggatcgg gccatcgcgg cggccggtgc
    18781 ggctccgccg acaatcccgg cggattctcc cccgccggca gccccccccc cccccccccc
    18841 cc
//
LOCUS       DAOQPA010000070        18647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1492_length_18647_cov_17.348376, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000070 DAOQPA010000000
VERSION     DAOQPA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18647
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_18647_cov_17.348376"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3014)
                     /locus_tag="PKX01_10735"
     CDS             complement(<1..3014)
                     /locus_tag="PKX01_10735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HMZ76646.1"
                     /translation="MPILPPPPYAELCCLTNFTFLTGSSHPEEFVDRARSLGYHALAV
                     TDECSVAGIVRAWDAWRRNPSNTLRLIVGSLIRLEDGPAVVLLATDRAAYGRLAALIT
                     RGRRAAVKGRYRLTRADLEDGLPGCLALLLPPDGPLDPPALANDAHWLATRFPGELWL
                     AAPLLLGADDAARRDTLAAVAAAAGIPVVATATPLMHAPERRRLADVLTALRHRRPLD
                     DAGYRLAANGERHLQPREVLALRHPPAWLAETLAVAARCTFALDSLRYEYPEEIVPPG
                     LTPTSHLRRLVNAGLARRYPPPGTIPAVVRTQVNAELALIAELGYEAFFLTVEDIVRF
                     ARSQSILCQGRGSAANSVVCYALGITEVDPAQATLLFERFISRERNEPPDIDVDFEHD
                     RREEVIQYLYAKYGRDRAALAAAVIRYRPRSALRDVGRALGFDTGQLDQITRRLAWWD
                     GRRVAPERLAEAGLDPASPQVHQLLDLADQLIGFPRHLSQHVGGFVIARGPLAELVPV
                     ENAAMAGRTVIQWDKDDLEALGLLKVDVLALGMLSAIRRSLDLIAAWQGRPFGLPDIP
                     REDPQVYAMLRAADAIGVFQVESRAQLSMLPRLQPRCFYDLVVEVAIVRPGPIQGGMV
                     HPYLQARERLARGESLYYPRPEIRPVLERTHGVPIFQEQVMQLAMVAAGFSAGEADQL
                     RRAMGAWGRKGQLHLYQARLAQGMAERGYPPEFAAALCRQIEGFGSYGFPESHAASFA
                     LLVYVSAWIKRFHPAAFLCALLNSQPMGFYSPSQLIQDARRQGVAVLPADVCTSDGDS
                     TLEAPPGAPQPAVRLGLERIKGIGRDAARRIATARRQAPFASVDELTRRARLDPADLR
                     ALAAGGALSQLAGHRRQALWAATGSQPAPGLLRDAAIPDLPPPLPAPSEAQNLVADYA
                     HLGFTLGRHPLSLLRSHLRAERFLPAREIADCPDRKLARAAGLVTCRQRPGTAKGILF
                     VTLEDETGLINVIVRPELVEAERRILLGATLLG"
     gene            3126..4244
                     /locus_tag="PKX01_10740"
     CDS             3126..4244
                     /locus_tag="PKX01_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76647.1"
                     /translation="MKRRIGIGLAAALLVTCPTTWATEAQSIEIKVEDIASLKKYPKV
                     ALIGYAIEQQNFLVKMSTSWGSTSSSTSEVTLEGVSTPAMQAATDRLYADLVKRLEAA
                     GLEVVKLDEVRNDPMFADLKGDKPQPSPSETSFVFDKSKGFKNSKALVFSPSGLPWHI
                     PSAHEEAARFGAGDKMSANLSRAFSGKQPVADVENALAKARGITLLKAYYVVGFGRAG
                     GRVSEMTSFNYVSNRSEKTISAAANATAELYLDKTDTRLALRVPGETPMLRMRNNSSP
                     AADGSGFIRLDKKLSAGADFAVGEPKNANSTETQVGNALSTTLAVVGGLAGIRGVGSA
                     STQEFVVTANEQRYVDTVEALIQTVQAEFVDRLAAAAK"
     gene            complement(4364..5146)
                     /locus_tag="PKX01_10745"
     CDS             complement(4364..5146)
                     /locus_tag="PKX01_10745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ76648.1"
                     /translation="MKILPSRLALALLALTATSAVLAADNLATVNGTAIPQARADVII
                     AEQRGQGAPDSDQLRAAVKEDLIRREVLSQEARKKGLEKNASVMAQMDLARQAVLIRA
                     YLQDYVKAHPVSDADVKAEYDRIKAQLGDKEYHARHILVDKEEDAKAIIAKLDKGEKF
                     DELAKQSKDPGSREKGGDLGWANPASFVKPFADAMVKLEKGKYTAAPVKTDFGYHVIR
                     LEDTRALKTPSFEEAKTQLKQRLEQQAVERHITELRGKATVK"
     gene            complement(5180..5443)
                     /locus_tag="PKX01_10750"
     CDS             complement(5180..5443)
                     /locus_tag="PKX01_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HMZ76649.1"
                     /translation="MSVPDTIRTRLQSLAPLTLDIVDDSALHAGHAGARAGGGHYRLS
                     IVSTAFAGRNTVARHRMIYDALGDMMRHEIHALAIHARTPEEA"
     gene            complement(5440..5739)
                     /locus_tag="PKX01_10755"
     CDS             complement(5440..5739)
                     /locus_tag="PKX01_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMZ76650.1"
                     /translation="MLYALIGEDAPGTLDTRMAVRPGHVARLQALQADGRLILAGPCP
                     AVDSPDPGPAGFSGSLIVAEFPTLADAEAWMAEDPYVLEGVFARTVVKPFKKVLP"
     gene            complement(5739..6275)
                     /locus_tag="PKX01_10760"
     CDS             complement(5739..6275)
                     /locus_tag="PKX01_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HMZ76651.1"
                     /translation="MKLLFDLFPILLFFIAYKFAGIYTATGVAIAATVGQIVWVWFHH
                     RKVDTMLWVSLVLVSVFGGATLIFQNPTFIKWKPTVLYWLFAVVLVGAPLILKRNLIR
                     AMLEAQMHLPDPLWGRLNLAWAGFFAAMGGLNLFVAYSFSEDAWVNFKLFGGMGLMLV
                     FVLAQGMILSRYIEEEPK"
     gene            complement(6330..6740)
                     /locus_tag="PKX01_10765"
     CDS             complement(6330..6740)
                     /locus_tag="PKX01_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HMZ76652.1"
                     /translation="MPKMPDPALSTPYESFGGEETIRRLVHRFYALMDALPEAWDIRQ
                     MHPADLSGSEQKLFMYLTGWLGGPPLYEEAYGHPRLRARHLPFTIGDTERDQWLLCMR
                     MALDECIPESPLRARILQAIEGLADHMRNRPAGG"
     gene            complement(6724..7434)
                     /locus_tag="PKX01_10770"
     CDS             complement(6724..7434)
                     /locus_tag="PKX01_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ76653.1"
                     /translation="MILFRQFFDTASCTLTYLLADPVTGDAVIIDSVREHVGAYISFL
                     QEQELQLDWILETHVHADHVTGAAALREFTGARSAVGAGGLVGCADRALEDGDSIVFG
                     NQVIRAIATPGHTPGCMSYHWRDRLFTGDALLIGGCGRTDFQGGDAGQLYDSITERLF
                     TLPDETLVYPGHDYRHARVSCIGQEKLANPRFAGKSRADFIELMGHLNLPKPTLMDQA
                     VPANLHCGRLAPLDAQDA"
     gene            complement(7473..8837)
                     /locus_tag="PKX01_10775"
     CDS             complement(7473..8837)
                     /locus_tag="PKX01_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76654.1"
                     /translation="MAIRPSGYRRFFLHAPRTLLWRIFLLVAALILVSVAMWSAIFNH
                     FDEAGRARQIAQTVSSIVNLTRSALVNADPALRRELLMDLATLEGIRIYPAEKQDRME
                     PLADTSLFHEITAEVRRQLGPDTRFASAWEGLPGFWVSFRLEGETPEDSTDFWVLLPP
                     ERVIRPHTREWLGWGAGALCLAMLGAYVIVSRLNRPLRQMAAAAAMVGRGETPPRLDE
                     DGPLELSDLARTFNQMSRDLQELDADRALILAGVSHDLRTPLARLRLGIEMSGASSAD
                     VDAMVSDIEDMDRIIGQFLEFSRSDGGEPLDTVALHELARELSTHYARRGTQLSIAAE
                     QPAFARARPQALRRAVINLVENALRYAGDSPALEVGIEQGQPCIAVLDRGPGIPPADV
                     ERVKRPFTRLDSARGNTKGSGLGLAIVDRIARGQQGELLLLPRDGGGLRAVIRLPAAP
                     KTAG"
     gene            complement(8845..9570)
                     /gene="ompR"
                     /locus_tag="PKX01_10780"
     CDS             complement(8845..9570)
                     /gene="ompR"
                     /locus_tag="PKX01_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HMZ76655.1"
                     /translation="MEKESYRILVVDDDVRLRDLLKRYLGEQGFNVKTVSDAAQMDRA
                     LLREHFDLMVLDLMLPGEDGLAICRRLRGTENTIPIVMLTAKGDDVDRIVGLEMGADD
                     YLPKPFNPRELVARIHAVLRRQPQTPPGAPATEDEDVSFGSVVVQLGTRILVRNGEET
                     LLTTGEFALLKVLLQHPRQPLSRDRLMELARGREYDVFDRSIDVQVSRLRKLIEDDPG
                     KPRYIQTVWGFGYVFVPDGNKHG"
     gene            9811..10131
                     /locus_tag="PKX01_10785"
     CDS             9811..10131
                     /locus_tag="PKX01_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76656.1"
                     /translation="MNRFSPTRFAHLRLGRRLLAGGTLVLILVSAAHADERRREAFIR
                     PPGPGQERPTRQIPAFEMDTLRRQEFPEGREPSRRWTSEERRQLRRDVHEAGRDLYVD
                     APRP"
     gene            10210..10899
                     /gene="tsaB"
                     /locus_tag="PKX01_10790"
     CDS             10210..10899
                     /gene="tsaB"
                     /locus_tag="PKX01_10790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMZ76657.1"
                     /translation="MKILAIESSTEAASVALLCGDVLLERDVPGRTHSETLLPAIRAL
                     LGEAGCPLNALDGVAFGAGPGAFTGLRLACGAAQGLAMGIDRPVVPVGTLEALASLYE
                     GDRIFVAMDARMAEVYFAAYRRVGTRLDEVLAPACARPEDVRLPASGSWAGVGSAFAA
                     YPDRLLPGLGDRLLAVDAAVQPRAGAVARLAAVRLARGEGLDATLAAPLYVRDKVALT
                     TAERLAQGGRA"
     gene            10926..11348
                     /gene="rimI"
                     /locus_tag="PKX01_10795"
     CDS             10926..11348
                     /gene="rimI"
                     /locus_tag="PKX01_10795"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HMZ76658.1"
                     /translation="MAETDLSWVAEQDRVLYPFPWVEQNFSDSMAAGYSCWLMRAGDA
                     PVGYAVLMRVLDEAHILNISVAADCQGRGFGRRLLGHLCAVALAAGGRQMFLEVRPSN
                     ASALALYARAGFETIGRRKGYYPAPEGREDALVMRLPL"
     gene            11345..12244
                     /locus_tag="PKX01_10800"
     CDS             11345..12244
                     /locus_tag="PKX01_10800"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMZ76659.1"
                     /translation="MSGRRDSVLREMGLAPLWRLRDRPAVDDACGQLADAVVDRAPLQ
                     AERSADTVPAAAGAVDLRIVPPLHSDARRGHAGLPPLAAGVAPAVTPEHVADGRIEGL
                     DWDALEATIRTCEACVLCQKRKQAVPGVGDRQADWLFVGEGPGAEEDERGEPFVGQAG
                     RLLDAMLASIDLRRGHDVYIANAVKCRPPHNRTPETAEIAACFPYLERQIALLQPRLI
                     VALGRPAAQALLNQEIKIGASRGRLFRYGGIPVIVTYHPAYLLRNLHDKGKVWEDLCF
                     ARRTMAELHARADAGAPENLPTI"
     gene            12272..12883
                     /locus_tag="PKX01_10805"
     CDS             12272..12883
                     /locus_tag="PKX01_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ76660.1"
                     /translation="MRFKLLLSMLLSVLLLSGCGYNQIQVNDEQVNAAWSEVLNQYKR
                     RADLIPNLVQVVQGYAEHEKDVLIKVTEARASVAGVKATPELVNDPEAMARFQKAQGD
                     LSGALSRLLVVSENYPNLKADANFRDLQAQLEGTENRITVARNRYIKAVQDYNVSVRT
                     FPNNLTAMVVGAKPKANFAVDNEQAISEAPKVNFGAPAKPAAQ"
     gene            12921..13826
                     /locus_tag="PKX01_10810"
     CDS             12921..13826
                     /locus_tag="PKX01_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ76661.1"
                     /translation="MNLRARLLAVCVFICAFVAGAWAEDLKPIPPLAARITDTVGLLP
                     GANRAALEARLADLEARKGAQVAVLIVASTQPEDIAAYSIRVVEAWKLGRTGVDDGVL
                     FLIARDDRRMRIEVGRGLEGAIPDAVAKRIVAERVAPRFKAGDFPGGIEAGLEALVTQ
                     IEGEEALPPPAPAATQGNDLEDFQGPAFIGCVVFAFIGGAILKAIFGRLIGATLTGGV
                     IAAVIWAVTAALVFGLIAGAIGFIFTLGAGGARGRHVGGSSWSGGGFGGAGGFGGGSS
                     DSSDSGGGWSGGGGDFGGGGASGDW"
     gene            13826..14323
                     /locus_tag="PKX01_10815"
     CDS             13826..14323
                     /locus_tag="PKX01_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ76662.1"
                     /translation="MEWRRILRHLAVPHWTVRCAFTSTVLSAIETAIRRSEATHRGEI
                     RFAVEGGLAWSDLRRGLTARQRAAKVFSDLGVWDTAGNTGVLIYVQWVDRRVEILADR
                     GIAACVPQAEWNAVCHGLEQAFKAGRYAEGCTAAVDAVGQLLATHFPAGADNPDELPD
                     RPVLL"
     gene            complement(14333..17533)
                     /locus_tag="PKX01_10820"
     CDS             complement(14333..17533)
                     /locus_tag="PKX01_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76663.1"
                     /translation="MTLPPVPASSGTMTGAGVRPAQPERCALVAAGLYAFTATAWLLL
                     TEHTAEGAAGVLIQDGLLLVGVTALGLYLGIRHFLAQVTERAAACEATAQAYRAFFEF
                     VPDAVWFFDTRSRRIVAANPAAGRLYGRLASQMEGADVATVLADSTSAEWNPGPCLLG
                     HRLADGTIRALDTTLYDQVFAGHPCRVVYVQEAAISRALPNRLEPSIADILDKVPLAL
                     YRYGAAHEHYDYVSARMTEWLGVSADALGQPGGWKHFAERVPDDDLGALWARIDAALA
                     QPGTGPVDLTVTYRLHTAGGALLHIQDAMTLLRNPDGSLRAIAGAASDHTAAQQAGEY
                     LGVTLRSIGDGVIATNAAGNITLMNTAAYVLTGWTEQDAVGKPLRVVLHLEPPTDGPA
                     SDRGLPRQIRLVDRVGEVRTVACCRTPIRGSASDLPLGEVIVLHDLTDERTVRDSAAG
                     DRARYLALVENSPVGILRFSRDLRIVYCNTRLATLFRSTPDQLVGLDLHGLEDDTVLA
                     TLRDVLSGQPGAYDGPFRSTMLPAPLNLSIRVTPELDADGEVIGGIGTVEDRTAFLET
                     EAQLRDTRERYVLAQRGTNEGLWDWDPRTRHLYLSARLLSLLGLTSDTLRTSGQEWQK
                     LIHPDDRPHVRADFIAHLRGRTEHFESEYRIADKDGRYHWVHARGLAHRNRRGRPVRV
                     VGSIGDITARKQAEEQLKAERDFSRSLIDSLPAPFFLFDQAGRLVLWNSFVKDLTGLS
                     DDELHHAEVEKLVVPEQEPILEHRLQVALTSGQASAEVLLRRRDKDPVPFLVVGRRVI
                     LDGVPHIIGVATDLTERKFAERAIKRLNTELERRVNERTSQLSAALSELESFSYSVSH
                     DLRAPLRAIEGYSTILGSDYADRLDDEAKELLRRVRAAVHRMGQLIDDLLTLSRVSRK
                     ELERRPVDLSLLAQAICDELRQQEPGRHVIVEIAPDLKVDADPSLMRTVLENLLGNAW
                     KFTGRVEVPEIRFSAVRHEGMDAFVVQDNGAGFDMRYRENLFRPFQRLHSDREFPGTG
                     IGLATVARIVRRHGGQVWAEGEVGKGAALYFSIGQS"
     gene            17633..18118
                     /gene="tadA"
                     /locus_tag="PKX01_10825"
     CDS             17633..18118
                     /gene="tadA"
                     /locus_tag="PKX01_10825"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HMZ76664.1"
                     /translation="MTESDLDRDTAYMRQAIELAREAGRHGEVPVGALVVGDGGIVGR
                     GFNRPIGTHDPTAHAEIMALRDASARLANYRIPGHTLYVTLEPCAMCAGAILHARIAR
                     VVFGATDPKTGVSGSVLDLFSEKRLNHHTTVVGGILAEECGGLLRDFFAMRRNKSVSG
                     A"
     gene            18122..18601
                     /locus_tag="PKX01_10830"
     CDS             18122..18601
                     /locus_tag="PKX01_10830"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HMZ76665.1"
                     /translation="MQILIDIPSQWLSLLADDGTCLRRYRVSTARNGPGERSGSHCTP
                     RGRHVIRARIGAGQPLGAAFRGRRPTGEVWSPEMAAAQPERDWILTRILWLSGREPGR
                     NRWGDVDSMRRYIYIHGTPDTEPMGVPLSIGCVRMRNREVVELFDLVPAGTPVEIRS"
BASE COUNT         2920 a         5752 c         6963 g         3012 t
ORIGIN      
        1 cccagcaggg tggcgccgag caggatgcgc cgttcggctt cgacgagttc cgggcggacg
       61 atgacgttga tcagcccggt ctcgtcctcc agggtgacga acaggatgcc cttggcggtg
      121 ccgggccgct ggcggcaggt gacgaggccg gcggcgcggg cgagtttgcg gtcggggcag
      181 tcagcgatct cgcgggcggg caggaagcgc tccgcgcgga ggtggctgcg cagcaggctg
      241 aggggatggc ggccaagggt gaagccgagg tgggcgtaat cggcgacgag gttctgggct
      301 tcgctgggag ccggcagcgg cggcggcagg tcagggatgg cggcgtcccg cagcaggccc
      361 ggagcgggct ggctgccggt ggcggcccac agggcctggc gacggtgacc ggcgagctgg
      421 gacagggcgc cgccggcggc gagggcgcgc aggtcggcgg ggtcgaggcg ggcgcggcgg
      481 gtgagttcat ccaccgatgc aaacggggcc tggcggcggg ccgtggcgat gcgtcgggcg
      541 gcatcccggc cgatgccctt gatgcgctcc aggccgaggc gcacggcggg ctgcggggcg
      601 ccgggcgggg cttcgagggt gctgtcgccg tcgctggtgc agacatcggc gggcagcacc
      661 gccacgcctt ggcggcgggc gtcctggatg agctgggagg ggctgtagaa acccatgggc
      721 tggctgttga gcagcgcgca caggaaggct gcggggtgga agcgtttgat ccacgcggac
      781 acgtacacca gcagcgcgaa gctggcagcg tgggattcgg ggaagccgta gctgccgaag
      841 ccttcgatct ggcggcacag ggcggcggcg aactcggggg ggtagccccg ctcggccatg
      901 ccctgggcca gacgggcctg gtataggtgg agctgaccct tgcggcccca ggcgcccatg
      961 gcgcgccgca gctggtcggc ttcgccggcg ctgaagccgg cggcgaccat ggcgagctgc
     1021 atgacctgtt cctggaagat gggcacgccg tgggtgcgtt cgagtacggg gcggatctcg
     1081 gggcgggggt agtaaaggga ttcgccgcgg gcgaggcgct cccgcgcctg caggtaggga
     1141 tgaaccatgc cgccctggat cggaccgggt cgcacgatgg ccacttccac caccagatcg
     1201 tagaagcagc gtggctgcag gcggggcagc atgctcagct gggcgcggga ttccacctgg
     1261 aacacgccga tggcgtcggc ggcgcggagc atggcgtaga cctgcgggtc ctcgcggggg
     1321 atgtcgggca ggccgaaggg gcggccctgc caggcagcga tgaggtccag gctgcgccgg
     1381 atggcggaca gcatgcccag ggcgaggacg tccaccttga gcaggccgag ggcttccagg
     1441 tcgtccttgt cccactggat gacggtgcgc ccggccatgg cggcgttttc gaccggcacc
     1501 agctcggcca gggggccgcg ggcgatgacg aagccgccca cgtgttggga caggtgacgg
     1561 ggaaagccga tgagctgatc ggccagatcg aggagttggt gcacctgcgg gctggccgga
     1621 tcgagaccgg cttcggcgag gcgttcgggg gcgacgcggc ggccgtccca ccaggcgagg
     1681 cggcgggtga tctggtcgag ctgaccagtg tcgaagccga gggcgcggcc cacgtcccgc
     1741 agggcgctgc gggggcgata gcggatcacc gcggcggcca gggcggcgcg gtcgcgaccg
     1801 tacttggcgt acaggtactg gatgacctcc tcgcgccggt cgtgctcgaa atccacatcg
     1861 atgtccggcg gctcgttgcg ttcccgggag atgaagcgtt cgaagagcag ggtggcctgg
     1921 gccgggtcga cctcggtgat gccaagggcg tagcacacca cggagttggc cgccgagccg
     1981 cgcccctggc acaggatgct ctggctgcgc gcgaagcgga cgatgtcctc gacggtgagg
     2041 aagaaggctt catagccgag ttcggcgatc agggcgagtt cggcgttgac ctgggtgcgg
     2101 acgacggcgg ggatggtgcc gggtggcggg tagcggcgcg ccaagccggc attcaccagg
     2161 cggcgcaggt ggctcgtggg ggtgaggccg ggcgggacga tttcctcggg atattcgtag
     2221 cgcaggctat cgagggcgaa ggtgcagcgg gcagccacgg cgagggtctc cgccagccag
     2281 gcgggtggat gacgcagggc gaggacctca cggggctgga ggtggcgttc gccgttggcg
     2341 gccaggcggt agcccgcgtc gtcgaggggc ctgcggtggc gcagggcggt aaggacgtcg
     2401 gccaggcggc ggcgctcggg ggcatgcatc agcggggtgg cggtggcgac cacgggaatc
     2461 ccggcggcgg cggcgaccgc ggcgagggtg tcgcgtcggg cggcgtcatc ggcgccgagc
     2521 aggagcgggg cggcgagcca gagctcaccg ggaaaacgcg tggcgagcca gtgggcgtcg
     2581 ttggcgagcg ccgggggatc cagcgggcca tccggcggca ggagcagggc gagacagccg
     2641 ggcagaccgt cctccagatc ggcacgggtg aggcggtagc gtcccttgac ggcggcccgg
     2701 cggccacggg tgatgagggc ggccagacgc ccgtaggcgg cccggtcggt ggcgagcagg
     2761 acgacggcag gcccgtcttc caggcggatg aggctcccga cgatgagtcg aagggtgttc
     2821 gacgggttcc gtcgccaggc gtcccaggcg cgcacgatgc cggcgacgga acattcgtct
     2881 gtcactgcca gtgcgtggta acccagtgat cgtgcacggt cgacgaactc ttccggatgg
     2941 gaagatcctg tcaggaatgt aaaattcgtg aggcagcaca actcggcata gggaggcgga
     3001 gggaggattg gcataggaaa actgtacaaa agaacagttt ttcggtcaat ctcacatgcc
     3061 ccgcatcgac ctgtcgcgcc gggttgggct tgttcgacaa cacatcatcc cggagcacga
     3121 catccatgaa gcgtcgaatc ggtatcgggc tggccgcagc cttgctggtc acctgtccca
     3181 cgacctgggc gacggaagcc cagtccatcg agatcaaggt tgaagacatc gcctccctca
     3241 agaagtaccc gaaggtggcc ttgattggct atgccatcga gcaacagaac tttctggtca
     3301 agatgtccac gagttggggc agcacgtcat ccagcacctc ggaagtgacc ctcgaagggg
     3361 tttcgacacc cgccatgcag gcggccaccg accggttgta tgccgatctg gtcaagcgcc
     3421 tcgaggcggc ggggctggaa gtggtgaaac tggacgaggt gcgcaacgac ccgatgtttg
     3481 cggacctcaa gggcgacaag ccgcagccgt ccccctccga gacgagcttc gtgttcgaca
     3541 aatccaaggg attcaagaac agtaaggcgc tggtgttctc gcccagcggt ctgccctggc
     3601 acattccttc cgcccacgaa gaagcggccc gcttcggcgc gggcgacaag atgtcggcca
     3661 acctgtcccg tgccttctcc ggcaagcagc ccgtggccga cgtggagaat gccctggcca
     3721 aggcccgtgg catcaccctg ctgaaggcct attacgtcgt gggcttcggc cgcgccggcg
     3781 gacgggtgtc ggaaatgacg tccttcaact acgtgagcaa tcgcagcgaa aagacgattt
     3841 cggcagcagc caacgcgacg gcggagctct atctggacaa gacggatacc cgtctggccc
     3901 tgcgggtgcc gggcgagaca ccgatgctgc gcatgcgtaa caacagctcg ccggctgccg
     3961 atggcagcgg tttcatccgc ctggacaaga agctgagcgc cggggcggac ttcgcggtgg
     4021 gtgaaccgaa gaacgcgaat tcgacggaaa cccaggtggg caacgccctc tcgaccaccc
     4081 tggcggtggt gggcgggctc gcgggcatcc gcggcgtggg ttcggccagc acgcaggaat
     4141 ttgtggtgac ggccaacgaa cagcgctacg tggatacggt tgaggccttg atccagacag
     4201 tgcaggccga gtttgtggac cgcctggccg ccgcggccaa gtaaatctgc ccgggcgggc
     4261 gtcgattgcc acgacgccgc ccgctgcggg cgccatccgt aaaaagccga ccggaatgac
     4321 ccggtcggct tttctttgag ggccgcctgc tgcagggcga tccttacttg acagtggcct
     4381 tgccgcgcaa ttccgtgatg tggcgttcaa cggcctgctg ctcgagacgc tgcttgagct
     4441 gcgtcttggc ttcctcgaag gacggcgtct tgagcgcgcg ggtgtcctcg agcctgatga
     4501 cgtggtagcc gaagtccgtc ttcacggggg cggccgtgta tttgcccttt tcgagcttga
     4561 ccatggcatc ggcgaacggc ttcacgaagc tcgcggggtt ggcccagccc agatcgccgc
     4621 ccttttcgcg ggagcccgga tccttggact gcttggccag ttcgtcgaac ttctcgccct
     4681 tgtcgagctt ggcgatgatg gccttggcgt cctcttcctt gtccaccagg atgtggcggg
     4741 cgtgatattc cttgtcgccc agctgggcct tgatgcggtc gtattcggcc ttcacgtcgg
     4801 cgtcgctgac ggggtgcgcc ttgacgtagt cctgcaggta ggcgcggatc agcacggcct
     4861 ggcgggcaag atccatctgg gccatgacgc ttgcgttctt ttcgagacct ttcttgcggg
     4921 cttcctggga cagcacctcg cgccggatca ggtcttcctt gaccgccgca cgcaactgat
     4981 cggagtcggg cgcgccctgg ccgcgctgct cggcaatgat gacgtccgca cgggcctggg
     5041 gaatggctgt tccattgacg gtggccagat tgtcggcagc gaggacggcg gaggtggccg
     5101 tcagggccag cagggcgagc gccagacggc tcgggagaat cttcatcgga tgttttcctt
     5161 gggtgtggga cagttgaaat caggcttctt cgggcgtcct ggcgtggatg gccagggcgt
     5221 ggatctcgtg gcgcatcatg tcgccgaggg cgtcgtagat catgcggtgc cgcgccaccg
     5281 tgttgcggcc agcaaaggcg gtggagacga tgctcaggcg gtaatggccg cctcctgcac
     5341 gggcgccggc atggccggca tgcagggcgc tgtcgtcgac gatgtcgagg gtcagcgggg
     5401 caaggctctg caggcgggtg cggatggtgt ccggcacgct cacggcagga cctttttgaa
     5461 gggcttgacg acggtacggg cgaacacgcc ctccagcacg taggggtctt cggccatcca
     5521 cgcctcggcg tcggccaggg tcgggaattc ggcgacaatc aggctgccgc tgaagccggc
     5581 ggggcccgga tcgggcgaat ccacggccgg gcaggggccg gccaggatca ggcggccatc
     5641 ggcctgcagg gcctgcaggc gggccacatg gcccggccgt acggccatgc gggtgtccag
     5701 ggtgccgggg gcgtcttccc cgatgagggc gtaaagcatt acttgggttc ctcttcgatg
     5761 taacgggaca ggatcatgcc ttgggcgagg acgaacacga gcatcagccc catgccgccg
     5821 aacagcttga agttgaccca ggcgtcctcg gagaatgaat aggcgacaaa aaggttgaga
     5881 ccgcccatcg cagcgaaaaa tccggcccag gccaggttga gccggcccca cagggggtcg
     5941 ggcaggtgca tctgggcttc gagcatggcc cggatcaggt tgcgcttgag tatcagcggc
     6001 gcgccgacga ggacgaccgc gaagagccag tacaggaccg tgggcttcca cttgatgaag
     6061 gtgggattct ggaagatcag ggtcgcaccg ccgaagaccg agacgagcac caggctcacc
     6121 cacagcatgg tgtcaacctt gcggtggtga aaccagaccc agacgatctg gcccacggtt
     6181 gcggcgatgg cgaccccggt ggccgtgtag atgccggcaa acttgtaggc aatgaagaac
     6241 agcaggatgg gaaacaggtc gaaaagcagt ttcatcggca ggcagtggga ttgaggcggg
     6301 gcgggcaggg cccggtgagt cggaggaggt cagccgcccg cggggcggtt acgcatgtgg
     6361 tcggcgaggc cttcgatggc ctgcaggatg cgtgcccgca atggggattc gggaatgcac
     6421 tcgtccaggg ccatgcgcat gcacaacagc cattgatcgc gttcggtatc gccgatggtg
     6481 aagggcagat ggcgggcacg cagacgcgga tggccatagg cttcctcgta gagcggaggg
     6541 ccgccaagcc agccggtgag atacatgaaa agcttctgct cggagcccga gagatccgcc
     6601 gggtgcatct gacggatgtc ccaggcctcc ggcagcgcgt ccatcagcgc gtagaagcga
     6661 tgcaccagac gacggatcgt ctcttccccg ccaaaggact cgtagggcgt ggacagggcc
     6721 gggtcaggca tcttgggcat ccagcggcgc caggcggcca cagtgcaggt tggccggcac
     6781 ggcctggtcc atcagggtgg gcttgggcag attgaggtgg cccatcagtt cgatgaagtc
     6841 ggcgcgggac ttgccggcaa agcgggggtt ggcgagcttt tcctggccga tgcaggagac
     6901 acgggcatgg cggtagtcgt ggccgggata gacgagggtc tcatcgggca gcgtgaacag
     6961 gcgttcggtg atgctgtcgt agagctgtcc cgcgtcacca ccctgaaagt cggtgcggcc
     7021 gcagccgccg atgagcaggg cgtcgccggt gaacaggcgg tcgcgccagt ggtagctcat
     7081 gcagccgggc gtgtggccgg gggtggcgat ggcgcggatc acctggttgc cgaagacgat
     7141 ggagtcaccg tcttccaggg cacggtcggc acagcccacg aggccacccg cgcccacggc
     7201 gctgcgggca ccggtgaact cgcgcagggc tgccgcgccg gtgacgtgat cggcgtggac
     7261 atgggtttcc aggatccagt cgagctgtag ctcctgctcc tgcaggaagg aaatgtaggc
     7321 gcccacgtgt tcgcgtaccg agtcgatgat cacggcatcg cccgtgacgg ggtcggcgag
     7381 aagataggtc agcgtgcagc tggcggtatc gaaaaactgt cggaacagga tcatggtggc
     7441 gagcgatgga cgatgaatgc ctgattctag cttcaaccgg cggttttggg cgcagcaggc
     7501 aggcggatga cggcccgcag gccaccgccg tcccgtggca gcaggagcaa ttctccttgc
     7561 tggccgcgcg cgatgcggtc gacgatggcc agtcccaggc ccgagccctt ggtgttgccg
     7621 cgggcggagt cgaggcgggt gaagggacgt ttcacccgct ccacgtcggc tgggggaatg
     7681 ccgggacccc ggtcgaggac cgcgatgcac ggctgtccct gctcgatgcc cacctccagc
     7741 gcggggctgt cccctgcgta gcgcagggca ttctccacca gattgatgac agcgcggcga
     7801 agggcctggg ggcgggcgcg ggcgaaggcc ggctgttcgg cggcgatgct cagctgagtg
     7861 ccgcggcggg cgtagtgggt ggagagctcc cgcgcaagct cgtgcagggc gacggtgtcg
     7921 aggggttcgc cgccgtcgct gcgggaaaat tccaggaact ggccgatgat gcggtccatg
     7981 tcctcgatgt cggagaccat cgcgtccacg tccgcgctgc tggcgccgct catctcgatg
     8041 cccaggcgca ggcgggccag gggtgtccgc agatcgtggg acacgccggc gaggatcagc
     8101 gcccggtcgg cgtccagctc ctgcaggtcg cgggacatct ggttgaacgt gcgggccagg
     8161 tcggacagtt ccagcgggcc gtcctcgtcc aggcgcggcg gtgtctcgcc gcgtccgacc
     8221 atggcggcgg cggcggccat ctggcgcagg gggcggttga ggcgcgacac gatgacgtag
     8281 gcgcccagca tcgccagaca cagggccccg gcgccccagc ccagccattc ccgcgtatgc
     8341 gggcggatca cccgctcggg cggcagcagg acccagaaat cggtgctgtc ctcgggcgtc
     8401 tcgccttcca ggcggaagct cacccagaag ccgggcaggc cctcccaggc ggacgcgaag
     8461 cgcgtgtccg gtccgagctg acggcgtact tcggcggtga tctcgtggaa cagggaggtg
     8521 tccgccaggg gttccatgcg gtcctgcttc tcggccggat agatgcgaat gccttccagg
     8581 gtggccagat ccatcagcag ttcgcggcgc agggccgggt cggcattgac cagggcgctg
     8641 cgggtgagat tgacgatgct cgacaccgtc tgggcgatct ggcgggcccg gccggcttcg
     8701 tcgaaatgat tgaagatggc cgaccacatg gccaccgaga cgaggatcag ggccgcgacg
     8761 agcaggaaga tgcgccacag cagggtgcgg ggcgcatgca ggaagaaccg ccggtaaccg
     8821 ctcgggcgga ttgccacggg ccgcttatcc gtgcttgttg ccgtcgggga cgaagacata
     8881 gccgaaaccc cacacggtct gaatgtagcg gggcttgccg gggtcgtctt cgatgagctt
     8941 gcgcaggcgc gacacctgca cgtcgatgga gcggtcgaag acgtcgtact cgcggccgcg
     9001 ggcgagctcc atcaggcggt cacgggacag gggctggcgc ggatgctgga gcagcacctt
     9061 gagcagggcg aattctccgg tggtgagcag ggtctcctcg ccgttgcgca ccaggatgcg
     9121 ggtgccgagc tgcaccacca cgctgccgaa actgacgtcc tcgtcttcgg tggccggtgc
     9181 gccggggggc gtctggggct ggcggcgcag cacggcatgg atgcgggcga ccagttcgcg
     9241 cgggttgaag ggcttgggca ggtaatcgtc ggcgcccatc tcgaggccga cgatgcggtc
     9301 cacgtcgtcg cccttggcgg tgagcatcac gatggggatc gtgttctcgg tgccgcgcag
     9361 gcgtcggcag atggccaggc catcctcgcc gggcagcatc aggtcgagga ccatcaggtc
     9421 aaagtgttcg cgcagcagcg ccctgtccat ctgggcggcg tcgctcaccg tcttgacgtt
     9481 gaaaccctgc tctccgaggt agcgtttgag caggtcgcgc aggcgaacgt cgtcgtcgac
     9541 gaccagaatc cggtagcttt ctttttccat ggacgcatcc tagcggctcg gcggatgggc
     9601 aaccagtgtc ccaccgcgca acgggtaagc aaacgtaagg gtcggcgcgg gcctaacaat
     9661 cccttacaaa gggtgtgggg cggcccccgg caggctgcca gaatggacgt acgtgtcaat
     9721 ctcccacacg ttccgtccgt aatgcggggc tgcgagtgtt tttgcggtgc cgcgcgcggc
     9781 aactcgggtt agcattggga ccccatgaaa atgaacaggt tttcccccac gcgattcgcg
     9841 catctgcgcc ttggtcggcg tctgctggcc ggcgggacgc tggtgctgat cctcgtttcg
     9901 gccgcccatg cggacgagcg acggcgggaa gccttcattc gaccgccggg gccgggccag
     9961 gagcggccga cacgccagat ccccgccttc gagatggaca ccctgaggcg ccaggaattc
    10021 ccggaaggcc gcgagccgtc ccggcgctgg acttcggagg agcggcgcca gctgcgtcgt
    10081 gacgtgcacg aggccggccg cgacctttac gtggatgcac cccggcccta gctgcgtgag
    10141 gccggcgggc ccggttcgcc gctgtgcccg ttggtggcgc ccgccgcggg cgatagaatg
    10201 ccgcccgaca tgaagatact tgccatcgaa tcgtccaccg aagccgcgtc ggtggcgctc
    10261 ctctgcgggg atgtcctgct cgaacgggat gtgcccggcc ggacccactc cgaaaccctg
    10321 ctgcccgcga tccgggcgct gttaggcgag gccggatgcc ccttgaatgc gctggacggc
    10381 gtggccttcg gcgccgggcc cggcgcattc accgggctgc gtctcgcttg cggggcggcc
    10441 cagggactgg cgatggggat tgaccggccg gtggtgccgg tcggcacgct ggaggcgctg
    10501 gccagcctgt atgaaggcga ccggatcttt gtagcgatgg acgcgcgcat ggccgaagtc
    10561 tatttcgctg cctaccggag agtcgggaca cgcctcgacg aggttctggc gcccgcgtgt
    10621 gcccggcctg aagacgtccg gctgccggca tccggctcgt gggcgggtgt tggttcggcc
    10681 tttgcggcgt atcccgaccg cctgttgccc ggactgggcg accgcctcct cgcggtggac
    10741 gcggcggtgc agccgcgggc cggcgccgtg gcgcggctgg ccgccgtccg tctggcgcgg
    10801 ggtgagggcc tcgatgccac gctggcggcg ccgctttacg tgcgtgacaa ggtggccctg
    10861 accaccgccg agcgtctggc ccagggcggg cgggcgtgag cgccgaaacc gtcgccttcg
    10921 tgccgatggc cgaaaccgac ctctcctggg tcgcggaaca ggatcgcgtc ctgtatccct
    10981 tcccgtgggt cgaacagaat ttctcggact ccatggccgc cggctacagc tgctggctga
    11041 tgcgggccgg cgacgcgccg gtcggctacg cagtgctgat gagggtgctc gacgaggcgc
    11101 atatcctgaa catcagcgtg gctgcggact gtcaggggcg gggattcggg cggcgcctcc
    11161 tcgggcatct gtgcgcagtg gcgcttgcgg cgggggggcg gcagatgttt ctcgaggtgc
    11221 ggccgtccaa tgcgtcggcg ctggccctgt acgcccgcgc aggatttgag accatcgggc
    11281 gccgcaaggg ctattacccg gccccggagg ggcgcgagga tgcgctggtg atgaggttgc
    11341 ctctgtgagc ggccggcgcg acagcgtgtt gcgggaaatg ggcctggcgc ccctgtggcg
    11401 cttgcgggac cgtccggccg tggacgacgc ctgtggccag ctcgcggacg cggtcgtcga
    11461 cagagcgccg cttcaggccg agcgctcggc ggataccgtc cccgcggctg ccggcgcggt
    11521 cgacctgcgg atcgtgccac cgctgcattc cgatgcccgg cggggccacg ccgggctgcc
    11581 gccccttgcg gccggcgtgg cgcccgcggt gacgccggag cacgtggccg acggtcgaat
    11641 cgaagggctg gactgggacg cgctggaggc gaccatccgg acctgcgagg cctgcgtcct
    11701 gtgccagaag cgcaagcagg ccgtgcccgg cgtgggcgac cgtcaggcgg actggctgtt
    11761 cgtcggcgaa ggaccgggtg cagaggaaga cgagcggggc gagccctttg tcggtcaggc
    11821 gggcaggctg ctggatgcga tgctggcaag catcgacctg aggcgcggtc acgatgtgta
    11881 catcgccaat gcggtcaaat gtcgtccgcc ccacaaccgg acgccggaga cggccgaaat
    11941 cgcggcctgt tttccttacc tggagcgcca gatcgcgctg ctccagccac gcctgatcgt
    12001 ggccctcggc cggccggcgg cccaggcgct gctgaaccag gagatcaaga tcggtgcttc
    12061 gcgggggcgg ctcttccggt acggggggat cccggtcatc gtcacgtatc acccggccta
    12121 cctgctgcgc aatcttcacg acaagggcaa ggtatgggaa gacctgtgct ttgcccgccg
    12181 gacgatggcc gagctgcacg cccgcgccga cgcaggggcg ccggaaaacc tgccgaccat
    12241 ctgatttaac tgccttcctg agggatgaac catgcgtttc aagctcttgc tgtcgatgct
    12301 cctgtccgtg ctgctgctct cgggctgcgg ctacaaccag atccaggtga atgacgagca
    12361 ggtcaatgcg gcctggtccg aggtgctcaa ccagtacaag cgccgggccg acctgattcc
    12421 caacctggta caggtggtgc agggctacgc ggagcacgag aaggacgtgc tgatcaaggt
    12481 gaccgaggcc cgcgccagcg tggcaggcgt caaggccacg ccggagctgg tgaacgatcc
    12541 ggaggcgatg gcgcgcttcc agaaggccca gggtgacctg tccggcgccc tgtcacgcct
    12601 gctggtggtc tccgagaact atccgaacct caaggccgac gccaacttcc gagatctcca
    12661 ggcccagctg gaaggcacgg aaaaccgcat caccgtggcc cgcaaccgtt acatcaaggc
    12721 ggtgcaggat tacaacgtgt cggtgcgcac cttcccgaac aatctgaccg ccatggtggt
    12781 gggtgccaag cccaaggcca atttcgccgt ggacaacgaa caggcgatct ccgaggcgcc
    12841 gaaggtcaat ttcggcgcgc cggccaagcc ggcggcccag tgacagtgcg tcgtcccttc
    12901 gacttcccgg ggcccgcggt gtgaacctcc gggcgcgcct gctggccgtg tgcgtcttca
    12961 tctgtgcctt cgtcgccggg gcatgggcgg aggatctgaa gcccatcccg ccgcttgcgg
    13021 cgcgcatcac cgacaccgtc ggcctgctgc ctggggcgaa tcgggcagca ctggaggccc
    13081 ggctggccga tctggaggcc cgcaagggtg cccaggtggc cgtgctgatc gtggccagca
    13141 cccagccgga ggacatcgcc gcctattcga tccgcgtcgt cgaggcctgg aagctgggcc
    13201 ggaccggggt ggacgacggg gtgctgttcc tgatcgcccg ggacgaccgg cgcatgcgca
    13261 tcgaggtggg acgcggcctg gaaggcgcga ttccggatgc ggtggccaaa cgcatcgtcg
    13321 ccgagcgggt ggcgccccgt ttcaaggccg gggattttcc cggcggcatc gaggcgggtc
    13381 tcgaggccct ggtcacgcag atcgaggggg aagaggcact gccgccgccg gcaccggctg
    13441 ccacgcaggg caacgatctt gaggattttc aaggccctgc attcatcggc tgcgtcgtgt
    13501 ttgccttcat cggcggtgcg atcctgaagg cgatcttcgg ccggctgatt ggcgcgaccc
    13561 tgaccggagg tgtgatcgct gcggtgatct gggccgtgac cgcggcactg gtcttcggcc
    13621 tgatcgccgg tgcgattggg ttcatcttca cgctgggtgc ggggggcgca cgcggacgcc
    13681 atgtgggcgg ctcctcgtgg agcggtggtg gcttcggtgg cgccggaggc tttggcggcg
    13741 ggagcagcga cagcagcgac tccggtgggg gatggtccgg tggcggcggc gatttcggcg
    13801 gtggcggcgc ctcgggagac tggtgatgga gtggcgcagg attctccggc atctcgccgt
    13861 tccgcactgg acggtgcgtt gcgcgttcac gtcgacggtg ctttccgcca tcgagacggc
    13921 gatccggcgc agcgaggcga cccaccgggg cgagatccgc tttgcagtgg agggcggtct
    13981 ggcgtggtcc gatctgcgcc gcggactcac cgcacgacag cgggccgcca aggtcttctc
    14041 ggatctgggt gtatgggata ccgccggcaa cacgggcgtg ctgatctacg tgcagtgggt
    14101 ggaccggcgg gtggagatcc tcgcggatcg aggcattgcg gcctgtgtgc cgcaagccga
    14161 gtggaacgcg gtgtgtcacg ggctggagca ggcctttaag gcgggccgct acgcggaggg
    14221 ctgcaccgcg gcggtggacg ccgtcgggca gctgctggcc acccattttc cggcgggggc
    14281 ggacaatccg gacgagttgc cggatcgccc ggtcctcctc tgacagcggt cctcagcttt
    14341 ggccgatgct gaagtagagg gcggcaccct tgcccacctc gccttcggcc catacctgcc
    14401 cgccgtgacg gcgcacgatg cgggcgacgg tggccagacc gatgccggtg ccggggaatt
    14461 cccggtcgga atgcaggcgc tggaaggggc ggaagaggtt ttcccggtag cgcatgtcga
    14521 agccggcgcc gttgtcctgc accacgaagg cgtccatgcc ctcgtggcgg acggcggaga
    14581 agcggatttc cggcacctcg acgcgtccgg tgaacttcca ggcgttgccc agcaggtttt
    14641 ccagcacggt gcgcatgagg ctggggtcgg cgtccacctt gaggtccggg gcgatctcga
    14701 cgatgacgtg gcggcccggt tcctgctggc gcagctcgtc gcagatggcc tgggcgagca
    14761 gtgacaggtc caccgggcgg cgctccagct ctttgcggct gacccgtgac agggtgagca
    14821 ggtcgtcgat gagctggccc atgcgatgca cggccgcacg cacgcggcgc aggagctcct
    14881 tggcctcgtc gtcgaggcgg tcggcgtagt ccgaaccgag gatggtgctg tagccctcga
    14941 tggcccgcag cggcgcgcgc aggtcgtggg agaccgaata gctgaaggat tccagttcgg
    15001 acagggcggc gctgagctgg gacgtgcgct cattgacgcg ccgttccagc tcggtgttga
    15061 ggcgcttgat ggcgcgctcg gcaaacttgc gctcggtgag gtcggtggcc acgccgatga
    15121 tgtggggtac gccgtcgagg atgacccggc ggcccaccac caggaaagga accggatcct
    15181 tgtcgcgacg gcgcagcagt acttcggccg aggcctgccc ggaggtgagg gcgacctgca
    15241 gacggtgttc caggatgggt tcctgttccg ggaccaccag tttttccact tccgcatggt
    15301 gcagctcgtc gtccgacagg ccggtaagat ccttgacgaa gctgttccac agcaccagcc
    15361 ggccggcctg gtcgaacagg aagaagggcg ccggcaggct gtcgatgagg ctgcgggaga
    15421 agtcgcgctc ggccttgagc tgttcctcgg cctgcttgcg ggcggtgatg tcgccgatgg
    15481 agccgaccac ccgtaccggg cggccgcggc ggttgcggtg ggccaggccg cgggcgtgca
    15541 cccagtggta gcgcccgtcc ttgtcggcga tgcggtattc ggattcgaag tgttcggtgc
    15601 ggccgcgcag gtgggcgatg aagtcggccc gcacgtgggg acggtcgtcc gggtggatga
    15661 gcttctgcca ttcctgaccg ctggtgcgca gggtgtcgct ggtgaggccc agcagggaca
    15721 gcaggcgggc cgacagatag aggtggcgcg tgcgcggatc ccagtcccac aggccttcgt
    15781 tggtgccgcg ctgggccagg acgtagcgct cgcgggtgtc gcgcagctgg gcttcggtct
    15841 cgaggaaggc ggtgcggtcc tccacggtgc cgatgccgcc gatcacctcg ccgtcggcgt
    15901 cgagttcggg cgtgacgcgg atcgacaggt tgagcggggc gggtagcatg gtcgagcgga
    15961 aggggccgtc ataggcgccg ggctggcctg acagcacgtc ccgcagggtg gcgagcaccg
    16021 tgtcgtcttc aagcccgtgc agatccaggc cgaccagctg gtccggcgtg gaccggaaca
    16081 gggtcgccag ccgggtattg cagtacacga tgcgcaggtc gcgggagaat cgcaggatgc
    16141 cgacggggga attctccacc agggcgaggt agcgggcccg gtcgccggca gcgctgtccc
    16201 ggacggttcg ttcgtccgtc aggtcgtgca ggacgatcac ctcgcccagg ggcagatcgc
    16261 tggcgctgcc ccggatcggt gtccggcagc aggcaaccgt ccggacctca ccgacccgat
    16321 ccaccaggcg gatctgacgg ggcaggccgc ggtcggatgc ggggccgtcc gtggggggct
    16381 ccaggtgcag aacgacgcgc aggggcttgc ccacggcgtc ctgttcggtc cagccggtca
    16441 gcacgtaggc cgcggtgttc atcaatgtga tgttgccggc ggcgttcgtt gcgatgacgc
    16501 catcgccgat gctgcgcagg gtgacgccca gatattcgcc tgcctgctgc gccgcagtgt
    16561 ggtcggacgc cgcgccggca atggcccgca ggctgccgtc cgggttgcgc agcaaggtca
    16621 tggcgtcctg aatgtgcagg agcgcgccgc ctgcagtgtg cagacggtag gtgaccgtga
    16681 gatccaccgg gcccgtgccg ggctgggcca acgccgcgtc gatgcgggcc cacagcgcgc
    16741 ccaggtcgtc atccggcacg cgttctgcga aatgcttcca gccgcccggc tggccaaggg
    16801 cgtcggcaga gacccccagc cactcggtca tgcgggccga tacgtagtcg taatgttcgt
    16861 gggcggcgcc gtagcgatag agggcgagtg gcaccttgtc gaggatgtcg gcaatggacg
    16921 gctcgaggcg gttcggcagc gcccgcgaga tggcggcttc ctggacgtag acgacccggc
    16981 agggatgtcc cgcgaagacc tgatcgtaaa gggtcgtgtc gagggcccgg atcgtgccgt
    17041 cggccaggcg atgccccagc aggcaggggc ccggattcca ctcggccgac gtgctgtccg
    17101 ccagcaccgt ggccacgtcc gcgccctcca tctgggacgc gagacggcca tagagccgcc
    17161 cggcggccgg gttggccgca acgatccggc ggctgcgtgt atcgaaaaac cacactgcat
    17221 ccgggacgaa ctcgaagaaa gcacggtagg cttgtgccgt ggcttcacag gccgcagccc
    17281 gttcggtcac ctgggcgagg aagtgacgga tgcccaggta gaggccgagc gccgtgacgc
    17341 cgacgagcag cagtccgtcc tgaatcagca cgccggcggc gccctccgcc gtgtgttcgg
    17401 tcagcaacaa ccaggccgtg gcggtgaagg catagagccc ggccgccacc agggcgcagc
    17461 gttccggctg cgcaggccga acgcccgcgc cggtcatcgt gccggaagac gccgggacag
    17521 gaggcagcgt catctgagcc gtcctttaag gccatgcggg agggtggggg ccggtgcttc
    17581 cacgttgctg agataaaatc cgccgattgc tttacgacta ttcactgttc caatgaccga
    17641 gtccgacctt gatcgggata ccgcctacat gcgccaggcc atcgaactcg cccgcgaggc
    17701 cggccgccat ggggaagtcc ccgtaggcgc gctggtggtc ggggacggag ggatcgtcgg
    17761 gcgcggcttc aaccgcccga tcggcaccca cgacccgacg gcccacgccg agatcatggc
    17821 cctgcgggac gcttctgccc gcctggcgaa ctaccgcatt cccggccata ccctgtacgt
    17881 cacgctggag ccttgtgcca tgtgcgcggg cgccatcctg cacgcgcgca tcgcacgggt
    17941 ggtattcggt gcaacggacc cgaagacggg tgtttcaggt agcgtactcg atcttttttc
    18001 cgagaaacgc ctaaaccatc acaccaccgt cgtcggcggc attcttgcag aggaatgtgg
    18061 agggctttta cgggattttt tcgcgatgcg ccgaaataag tcggtatcag gggcataaat
    18121 tttgcaaatt ctcattgata tccccagcca atggctgtcc ctcctggccg acgatggcac
    18181 ctgcctgcgg cgctaccgcg tttcgacggc ccgcaacggg cccggcgagc gctcgggcag
    18241 ccactgtacg ccccgcggcc ggcatgtgat ccgcgcccgg atcggcgcgg gccagcccct
    18301 tggcgcagcc ttccgcgggc ggcgtcccac cggcgaggtg tggtcgccgg aaatggccgc
    18361 ggcgcagccg gagcgggact ggatcctcac gcgcatcctg tggctgtcgg gccgggagcc
    18421 ggggcgcaac cgttggggcg acgtggattc catgcgccgt tacatctaca tccacggcac
    18481 gccggacacc gagcccatgg gcgtgcccct gtccattggc tgtgtgcgca tgcgcaaccg
    18541 ggaggtggtg gaactcttcg atctggtgcc ggccggcacg ccggtggaga ttcgctcgtg
    18601 agcccggccc aggacgccgt gccggaggtc atcctcgggg cgtggga
//
LOCUS       DAOQPA010000071        18363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1522_length_18363_cov_18.824831, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000071 DAOQPA010000000
VERSION     DAOQPA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18363
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_18363_cov_18.824831"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKX01_10835"
     CDS             complement(<1..319)
                     /locus_tag="PKX01_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMZ76666.1"
                     /translation="MTPLTVNGVRHRVDVEPDTPLLWALRDALLLTGTKYGCGAGLCG
                     ACTVHVDGQPVRSCSTPVSAAAGKKITTIEAADQSRVGKAVQAAWRKLDVVQCGYCQS
                     GQIM"
     gene            541..1458
                     /locus_tag="PKX01_10840"
     CDS             541..1458
                     /locus_tag="PKX01_10840"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ76667.1"
                     /translation="MYGAGLSPALPLHAADRRTSPLAALIERYCPDDGHRETQVPGLD
                     LFRASDAGSPVCGVYRPVLAVIAQGCKRIGLGDETYVYDSQHCLVSAVDLPVTAQIVG
                     ASRERPYLCAVIELDPRRIADVALAMDLPRPAPAAPRGLGVSPLTPAVADAVLRLVQL
                     LETPEDIPFLAPLIERELLYRLLAGEQGLRLRALATADSQSQRTARAMEWIRHHYHLP
                     LSIDALAETANMSKSSLHHHFKALTAMSPLQYQKQIRLQEARRILLTEDADAATVARR
                     VGYESPSQFSREYRRQFGAPPLRDIARLR"
     gene            1593..2306
                     /locus_tag="PKX01_10845"
     CDS             1593..2306
                     /locus_tag="PKX01_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ76668.1"
                     /translation="MTEDLHARPAVVPPGFFDPPDEDDAPAHASDGDDAPLAVRPGPL
                     VDADPQRLRELLARVADEDEDALAALYEATGSRVYGLALRILRDPAAAEEVTEDAYFQ
                     VWRQARRYDPARGRPLAWLLTIVRSRALDHLRRDDPALPHPEPVSLLDAEPEHTDNPQ
                     NLLEACRDHDRLHAALAGLDPVPRQLIALVFFRGLTHEEAADHARMPLGTVKSHVRRA
                     LLALRAVLAADLDRSYSGS"
     gene            2303..3127
                     /locus_tag="PKX01_10850"
     CDS             2303..3127
                     /locus_tag="PKX01_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ76669.1"
                     /translation="MKPTYPLDPDQPDASADVEARILESLTPLPLPEARGRALASRLQ
                     ERIRASRDAGRGLITLRRDQGHWAPLVPGVRVKRLSGGQRAVLLDLAPGAVIPFHRHH
                     EDEECVVLRGSAQLGDVTVHPGDYHLATTGSRHGVVRSETGALLFLGGTPIGHGLEVA
                     RDVVTALLPGRGQAPLTVPRDEGPWTDPAPGLHLKLLRDDGHGRSLLVRLEAGCRADL
                     AELAGHGECLMVEGEAFAGDTLLQAGDYQLAPQGVTRPTLSSDVGGLLFVRTGLRV"
     gene            complement(3117..4307)
                     /locus_tag="PKX01_10855"
     CDS             complement(3117..4307)
                     /locus_tag="PKX01_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HMZ76670.1"
                     /translation="MLDAVEEIRAFNAGRDPERLVLKFRNMRSDPFVFLRATCHLFYR
                     RLPELPDSPAVWCCGDLHLQNFGSYKGDNRLVYFDLNDFDEGALAPAHWELVRFLTSV
                     HAGAPSLGVKRKEAAALCGDFLDAYRERLVAGKALWVERDTASGLVHQLLDGLRKRER
                     AAFLDGRTVRKGKKRLIRLDNGKALPVTEKQRERIAQFMAGVARTRDDPAFYEVLDVA
                     RRIAGNGSLGVDRFVILVRGKGPPDGHYLLDLKEALPSALLPVLKTPQPAWKSEAARV
                     VAVQRRMQAVSMAFLLPERLGKRAYLLRELQPTEDRVTLDAARSGLPALREAVATMGH
                     IVASAQLRSSGRDGSAIADQLIEFGAARWRKSLLAAAEASAKQLRADWKTFCAAYDDG
                     VFTP"
     gene            complement(4355..4810)
                     /locus_tag="PKX01_10860"
     CDS             complement(4355..4810)
                     /locus_tag="PKX01_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76671.1"
                     /translation="MKRLLCLLLAASGAAMAAPADPLTAVAEGAVYAQTRCYRLIHHD
                     TFDYEDCLTALLKDERRPTPRRLGMEYFGWVGAQNSARLGMRGAEQSAYEFLGRFRAT
                     QKRLRIGDEALCASVAGDCTVRIARMKQMEAAPRPRPAPGEGDDERPHH"
     gene            complement(4878..6692)
                     /locus_tag="PKX01_10865"
     CDS             complement(4878..6692)
                     /locus_tag="PKX01_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ76672.1"
                     /translation="MKRPVSTKMAAWLAALSTAWAVTGCGGGASSSESAAPVSVKLIA
                     MNDFHGNIEVPAANNGGSVVLKDPANAAGTTVRSGGAAYLATLIKQLKAQNPYSIVVG
                     AGDMVGASPVTSTLTHDEATVDILNQIGLEVTSVGNHEFDHGKTELLRLQNGGCYPGG
                     TIGQDTCIKGGSFSGATYKWLSANVVDSSSGQSLFPATYVKKFGPVSVGFIGLTLKGT
                     PQVVTSTGVAGLDFQDEATTINKYAASLKASGVNAVVVLIHQGGQTTASTLNDKTCPN
                     LTGDILPIVDALSSNVDVVVSGHTHQEYVCTRNGKLLTSTGFYGSAVTEINLTLLPGG
                     GVLTRDANTRPVINDLNTTVPAGFSILTKDATIDAAVQSYVSLSATLKNQVVGSITAD
                     IKRALLSSSSTPTRDETAEGAMGDVMADVYLAGGPKADIAFINPGGVRADLIYKNGGA
                     VTYGDLLTVAPFGNTLVTVDLTGADLVRLLEQQWEAPNCAAKTGANGCGRMLQPSSTF
                     SYTWDASKPAGAAVGSGNRVVAGSLKLNGVAMDLTKTYRVTLNSFMAPGPGDNFSVVT
                     ASGKNVTPSGVIDIDAFVGYMKAHPSLAPPAARITRLN"
     gene            complement(6766..8787)
                     /locus_tag="PKX01_10870"
     CDS             complement(6766..8787)
                     /locus_tag="PKX01_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HMZ76673.1"
                     /translation="MSAALPPEVRVAVPSRLLRGRCRPVVALVGLPRTGKSTLFDAAS
                     STAVQSGQLDGSALGYRACVVNVGLEQAGVVDLPPLRTLLDLDEADRRVLTALLEGEP
                     GEGGFRAPDVLVHVVDATALTRDLALSQELCLLGKPLVIALNRLDEAREKGIYINTAA
                     LAEALGVTVVPTVAHMGKGISALFEAALEAVRRSVCPVPQPASPHLRQALHGFDAVLD
                     LPEVEAAFPVPRALLLTQLAKADTLLAREFAERFPALMPRIAAARKAAGAQLPRSLSE
                     ELFADRHYRAALLHEQVTRLGHAAEKAGWKRQLDRLFLHPRWGLIGTLAVFALVLYVV
                     FEVSTGLDSVTSAPLAAWVGEWQPDTTAGVIGRAIADGLVGLVGIVVPYMLPLVLLLV
                     GLEESGIMHRVAFVVDRGFHRIGLHGGVAVPFLLGLGCNVPALSAVAASSAGRERIVA
                     SLLITFVPCSARSAIVLAIGGKYLGWEGVLGIFLLTMLVIAVGGRLLTRRYVQASPGM
                     IQAIPAYALPRWRKVLAVTWERTSDILTIVTPLLVLGSVVLALLTHFGADEVINTLLV
                     PLTSWSLGLPVVLGVPILFGVLRKELSLLMVYQALGTQEIAPLLDWVQIATFLVFITF
                     YVPCVSTFAVMLKTVGRREAVFSLGLSVAVALGIAVACRILLHGVNLLL"
     gene            complement(8976..9623)
                     /locus_tag="PKX01_10875"
     CDS             complement(8976..9623)
                     /locus_tag="PKX01_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76674.1"
                     /translation="MMPTRILIIEDHVLVREAMALTLAQVGEGVFCQQASSAAEAIAL
                     LEEDPDCDLLVVDLMLPEMNGFSLLGVIAKRFPDVPALVVSALDDRESVHRAMKAGAS
                     GFVSKASPSNTLIEAVRTVLDGGVFTPEATSGSDGSGRTRRASQAFAERFQLTAAQGR
                     VLELVAQGKSNRDIADLLGLSEGTVKVHVSAILRSLGVTSRAQALLLMTRAGLRV"
     gene            9743..11806
                     /locus_tag="PKX01_10880"
     CDS             9743..11806
                     /locus_tag="PKX01_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76675.1"
                     /translation="MPRIAFAPFNAAGLLTSLFSLLTPSAAGAAPNGEDSFLDDLPVV
                     LSATRLTQAPADAPGSVTVLDRDMIRASGARNISELFRLVPGFQVAMQTGNTPQVTYH
                     GLSDEAPRRMLVQVDGRSVYSAYFISGVEWNQIGVDLDDIERIEVFRGSNSAAYGSNA
                     FLGVANIITRPAAETQGVALRLRGGSDGVNDQGVRIGQRLGDVDLRVTAARSHDNGFA
                     DLNDWRRNELANLRADWHIQHDHQVEFQAGWTRNEFGTGKANNLSDPERSAELSTGFG
                     LLRWRHTPQPDEELSVTYYHQQETGKDAYPYTVIVPPHLTGLPVNLQIPFFFEYGYNV
                     TRDDLEAQRITSLSPTLRAVYGGGLRSDRSHAPLRFNTADTVRSDTRHLFGTLEWRAS
                     PAWLFNLGAMLENSSLTGTSVDPRISANYHFSDISTWRASINRSHRHPSAFEQKANMV
                     FRNSAAFPTPIGAVPAGTVISQTFRPSPDITAERITTYEIGYLAELRSLATTIDARAF
                     VERARNLIEMNIENSTIGLVPRDNVRYFANTGEADIRGVELSGTWRPAAQTWVNLTHT
                     ELRIAGPTVTAAQAARQSTPYVSNTAPKHTSTVFAAWEFVPRWQVSASKRWVGSMSWY
                     QDDPHAIPMYRQLDVRIAHRFQTAYGRGEVALVGQNIDGGEQTFTSTAGVWGSRTFAT
                     LSLEL"
     gene            11817..12737
                     /locus_tag="PKX01_10885"
     CDS             11817..12737
                     /locus_tag="PKX01_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HMZ76676.1"
                     /translation="MGCTIRRFLLILLTLAGLASPARAALVALVSADEGAAFSEAADA
                     ISAELRGAGHRVQSFTLPLRADDTGFASAALIVTLGSRAAQLVSTLPQHGPVLHTLLP
                     RSAFDRLPRPDDPRRVSAVYIDQPAGRQLELIRQALPDWQRLAVITGRDSAELGTRLQ
                     AAAREHRSLRVQTDIALGERDIYPVLQRMLAEPAILVAVPDATLFNSNTISNILLTAY
                     HHRSPVVGFSAAYIKAGALLALYSTPAQIGQQAGEVARQALASGTLPPPQYPRQFRVG
                     TNAYVARSLGITLEDPNALRDRLERTEGTP"
     gene            12734..14629
                     /locus_tag="PKX01_10890"
     CDS             12734..14629
                     /locus_tag="PKX01_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76677.1"
                     /translation="MSARDLDIGTRALVIAVLPAFVVAVVLIAYFTHSRLNDLEDAHS
                     QRGKALARQLAAASSYVVFTGNKDALGKLAAEILDEPDVVRVAVRGPGKDTLTDLRKP
                     APSDSGALRLGNADSDTLAFQEAIHSPGQLFDDPFVGAEGAGSELHAQQGEVIVELSR
                     DALHGEETRLLRNAVLMVFAVLLGSVVLALRLSRGITRPIQRIASTVARIGQGERAAR
                     VRVEGGHTLRALGQGVNDMADRIAAASENLERQIEAATAELLEKKNEAELANQAKTRF
                     LAAASHDLRQPMHALGLFVAELGQKPHAPDTCTLVEQIAMSAETMEDLLDSLLDISRL
                     DAGALEPQIKPFPLQPLLERVDVDYRREAERRGQRLRLRPTDLWVSSDPLLLERILHN
                     LISNALRYAPGGTVLVACRRRGAQVTVEVRDNGPGIAPDAQEAIFHEFVQLDNPERNR
                     AKGLGLGLAIVRRLTTLLGHPLGLRSRPGRGALFSVTLPVTAPRPQDLSPPSSAARGL
                     AGLHVALIDDDPLAAASTLSLLESWGCLVTAADTLEHMYAALQRSEMSPDIMLCDHHL
                     DGALDGVAVVARLRQLLGTDLTAIILSSDTTLADSPRVRDNGIRVLRKPVRPARLRAL
                     LQRKTGS"
     gene            14698..15117
                     /locus_tag="PKX01_10895"
     CDS             14698..15117
                     /locus_tag="PKX01_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76678.1"
                     /translation="MSADNTMQDPMEFMRNMWSKMGFSLPGMVTPTLDLEELDRRIND
                     MKTVENWLKMNLSSLQMATQGLEMQRATLAAMQAMSKVATEGAGKVDEGTPNPFASTM
                     WPWNFVPAAAEAAKAAAAPAESAEPAAKPAAKSKSAK"
     gene            complement(15162..16070)
                     /locus_tag="PKX01_10900"
     CDS             complement(15162..16070)
                     /locus_tag="PKX01_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMZ76679.1"
                     /translation="MQSFLLLLPDFALIALGVVLRRFGRLEDGFWAGVEKLVYFVFFP
                     ALLFHALARAPIDLSAAAPLFAVGLLTMASGFALALLGRPLMGLGEMAFASRVQCAYR
                     FNTYIGIAVAGTLHGAAGVAAMGALCGAMVPFANMAAVGFLAHHGQGRLLRELARNPL
                     VIATLAGLAFSTAGLHLPGPVLPFLQRLADAAVTLGLIAVGAALRIGQREGRPVGMVY
                     LCAVKLLALPAVAWGLAGAFGLEGLAFATAVLFAALPTASSAYILAMRMGGDGPGVAW
                     LISATTVAAAVTLTGWLTALRGVAAG"
     gene            complement(16262..17497)
                     /locus_tag="PKX01_10905"
     CDS             complement(16262..17497)
                     /locus_tag="PKX01_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ76680.1"
                     /translation="MNYYDARETRDPAERERDLLARLPVQVAHAQARTPAFARLLAEV
                     DAAAVTSREALASLPVIRKSELSALQKAARPFGGLAALGWGAGCARVFASPGPIYEPE
                     GARKDYWRLARAFYAAGFRAGDLVHNTFSYHFTPAGSMMETAAHALGCTVFPAGVGQT
                     EQQLAAMLDLRPDAYAGTPSFLRILLDKAAEQGLRLDSLKKAFVSGEAFPPSQRDALA
                     AAGIQAFQAYATADLGLVAYETPAREGMVVDEDVIVEIVRPGTGDPVAPGEVGEVVVT
                     TFNPDYPLIRFGTGDLSAILPGASPCGRTNARIRGWLGRADQTTKVKGMFVHPGQVAA
                     VLKRHPEILRGRLVVDNPDLNDRMTLHCEVAGDGAGLAEAVVASLRELTKLRGEVQCC
                     APGSLPNDGKVIDDVRRYA"
BASE COUNT         3007 a         6900 c         5884 g         2572 t
ORIGIN      
        1 tcatgatctg gccggactgg cagtagccgc actggaccac gtccagcttg cgccacgccg
       61 cctgcaccgc cttgcccacg cgggactggt cggccgcctc gatggtggtg atcttcttgc
      121 ccgccgcggc cgacaccggg gtgctgcacg aacgcaccgg ctggccgtcc acatgcacgg
      181 tacaggcgcc gcagaggccg gcgccgcagc catacttggt accggtgagc agcagggcat
      241 cgcgcagggc ccacagcagg ggcgtatcgg gttcgacatc cacgcggtgg cggacgccgt
      301 tgacggtgag cggggtcatg gtcgtttctc cgttgggatc ggggcgggcc gtcggccgac
      361 aggcgcccgt gacgagaatc aggggcgaca gggacgcggc ggatctgcgc tgaactgtag
      421 cccaaggccc tgccggacgc attgcagaaa gctgccgatt tattgcccga tcctccgatc
      481 atggcgcagc ccacgtccgg gcaggctaca cttcagcggc atcctcagag gacagaaacc
      541 atgtacggag caggcctttc ccccgccctc cccctccatg ccgccgaccg gaggacgagc
      601 ccgctggcgg cgctgattga acgatattgc ccggacgatg gccatcgcga aacgcaggtg
      661 ccgggcctcg acctgttccg ggccagcgac gcgggatcgc cggtgtgcgg ggtgtatcgc
      721 cccgtgctgg cggtgatcgc ccagggctgc aaacgcatcg gcctcggcga cgagacctat
      781 gtgtatgaca gccagcactg cctcgtatcg gccgtggatc tgcctgtcac cgcccagatt
      841 gtcggcgcca gccgggagcg accctacctg tgcgccgtga tcgaactgga cccgcggcgc
      901 atcgccgacg tcgccctcgc catggacctg ccccgcccgg cacccgccgc accccgcgga
      961 ctcggcgtca gtcccctcac cccggcggtg gccgacgccg tgctgcgtct cgtgcaattg
     1021 ctggagacgc ccgaggacat tcccttcctg gcccccctga tcgaacggga gctgctctac
     1081 cgtctgctcg ccggcgaaca aggcctgcgc ctgcgcgccc tggccaccgc cgacagccag
     1141 agccagcgca ccgcccgggc gatggaatgg atccgtcacc actaccacct gcccctgtcc
     1201 atcgacgccc tggcagagac ggccaacatg agcaagtcgt ccctgcacca ccacttcaag
     1261 gccctcaccg ccatgagccc gctgcagtat cagaaacaga tccgcctgca ggaggcgcgg
     1321 cgcatcctcc tcaccgagga cgccgatgcc gccacggtgg cgcggcgggt cggctacgag
     1381 agcccgtccc agttcagccg cgaataccgt cgccagttcg gtgccccgcc cctgcgcgac
     1441 atcgcccggc tgcgctgacg ggggctgcat ccggatcggc tgcgtctgcg tagaccgaaa
     1501 agacaccccg attccattcc cgacctccgc agcgtgtgca cgagggagcc gaccaccatg
     1561 acgcagccac cgatccgccc cggagtccgg ccatgaccga agacctccac gcccgccccg
     1621 cggtcgtgcc gcctggcttc ttcgacccgc ccgatgagga cgacgccccc gcccacgcgt
     1681 ccgacgggga cgacgcgccc ctcgccgtcc ggcccggccc cctcgtcgat gccgacccgc
     1741 aacgcctgcg cgaactcctc gcccgcgtcg ccgacgagga cgaggacgcc ctcgcggcac
     1801 tctacgaagc caccggcagc cgcgtgtacg ggctggccct gcgcatcctg cgcgacccgg
     1861 ccgccgccga ggaggtcacc gaggacgcct acttccaggt atggcgccag gcgcgccgct
     1921 acgacccggc ccgcggccgc cctctggcct ggctgctcac catcgtccgc agccgcgccc
     1981 tcgaccacct gcgccgggac gatcccgccc tgccccaccc ggagcccgtc agcctcctcg
     2041 acgccgagcc ggaacacacg gacaatcccc agaacctgct cgaagcctgc cgcgaccacg
     2101 accggctcca cgccgccctg gccgggctgg acccggtgcc gcgccagctc atcgccctgg
     2161 tgttcttccg cggcctgacc cacgaagagg ccgccgacca cgcccgcatg cctctgggca
     2221 ccgtcaaatc ccatgtgcga cgcgccctgc tcgccctgcg tgccgtcctg gccgcggacc
     2281 tcgaccggag ttacagcgga tcatgaagcc cacctacccc ctcgaccccg accagcccga
     2341 cgcgagcgcc gacgtggaag cccgaatcct ggaatccctc accccgctgc ccctgcccga
     2401 ggcacgcgga cgcgccctcg ccagccgtct gcaggaacgc atccgcgcca gccgcgacgc
     2461 cggccgcggc ctcatcaccc tgcggcgcga ccagggccac tgggcaccgc tggtgcccgg
     2521 cgtccgcgtc aagcggctgt ccggtggcca gcgcgccgtg ctgctcgatc tggcgcccgg
     2581 cgcggtgatc cccttccacc gtcaccacga agacgaggag tgtgtggtgc tgcggggctc
     2641 ggcgcagctg ggcgacgtca ccgtacatcc cggcgactac cacctggcca ccaccggcag
     2701 ccgccacggc gtcgtgcgct ccgaaaccgg cgctctcctg ttcctgggcg gcacccccat
     2761 cggccacggc ctggaagtgg cgcgggacgt ggtcacggcc ctgttacccg gtcgcggtca
     2821 agcccccctc accgtgccgc gggacgaggg cccctggacc gatccggcgc cgggtctgca
     2881 cctgaaactg ctgcgcgacg acggccacgg ccgctcgctg ctcgtgcgcc tggaagcagg
     2941 ctgccgggcc gatctggcgg agctggccgg ccacggggaa tgtctgatgg tggagggcga
     3001 agccttcgcc ggcgacaccc tgctgcaggc cggcgactac cagctcgccc cgcagggcgt
     3061 gacgcgcccg accctgagca gcgacgtggg cggactgctc ttcgtgcgca ccggtctcag
     3121 ggtgtgaaca ccccgtcgtc gtaggcggcg caaaaggtct tccagtcggc gcgcaactgc
     3181 ttcgcactgg cctcggccgc cgccagcagg gatttgcgcc agcgcgcggc accaaactcg
     3241 atgagctggt cggcaatggc cgaaccgtcg cggccgctgc tgcgcagctg ggcgctggcc
     3301 acgatgtggc ccatcgtggc aacggcctcc cgcagggccg gcagaccact gcgggccgcg
     3361 tccagggtga cccgatcctc ggtgggctgg agttcgcgca gcaggtaggc gcgcttgccg
     3421 aggcgttcgg gcaacaggaa ggccatggac accgcctgca tgcgccgctg caccgccacc
     3481 acccgggccg cctcgctctt ccaggccggc tggggcgtct tgagcacggg cagcaaggcc
     3541 gagggcagcg cctccttcag gtcgaggaga tagtggccgt ccggcgggcc tttgccacgc
     3601 accaggatca cgaagcgatc cacccccagg ctgccgttgc cggcaatccg ccgggccaca
     3661 tcgagcactt cgtagaacgc cgggtcgtcg cgggtcctgg ccacgccggc catgaattgc
     3721 gcgatccgct cgcgctgttt ttcagtcacc ggcagggctt tgccgttgtc gaggcggatc
     3781 agacgcttct tgcccttgcg cacggttcgg ccgtcgagaa aggctgcacg ctcgcgcttg
     3841 cgcaggccgt cgaggagctg atgcaccagc ccggatgccg tgtcccgctc cacccacagg
     3901 gctttgccgg cgaccagacg ttcccggtag gcgtcgagga agtcgccgca caaggccgcc
     3961 gcctccttgc gcttgacgcc cagggacggc gcgcccgcat ggacgctggt caggaagcgc
     4021 accagctccc agtgggccgg cgccagcgcg ccctcgtcga aatcgttgag gtcgaagtac
     4081 accagccggt tgtcgccctt gtagctgccg aagttctgca ggtgcagatc accgcagcac
     4141 cacacggcag gggagtccgg cagctccgga aggcgccggt agaagagatg gcaggtggcg
     4201 cgcaggaaca cgaagggatc gctgcgcatg ttgcggaact tgaggaccag gcgctcggga
     4261 tcgcgaccgg cattgaaggc acggatttcc tcgaccgcat caagcatggc gcatctcccc
     4321 ggcatgtgac caaggcattc tacgcgccac gcgctcagtg atgagggcgt tcgtcgtcgc
     4381 cctcgcccgg cgccgggcgg ggacgcggcg cggcttccat ctgcttcatg cgggcgatgc
     4441 gcaccgtgca atcgccggcc acgctggcgc aaagggcctc gtcgccgatg cgcaggcgct
     4501 tctgggtggc ccggaagcgg ccaaggaatt cgtaggcgct ctgttcggcg ccgcgcatgc
     4561 cgaggcgggc cgagttctgg gcaccgaccc agccgaagta ctccatgccc aggcggcggg
     4621 gcgtgggacg gcgttcgtcc ttgagcaaag ccgtgaggca gtcctcgtag tcgaaggtat
     4681 cgtggtggat caggcggtaa cagcgggtct gggcgtaaac agccccctcg gcgaccgcgg
     4741 tgagcggatc ggcgggcgct gccatggcgg cgccgctagc cgccagcagc aggcagagca
     4801 gacgtttcat gaggcctcct caaacggcaa accggatgtc ggcccgcgcc gacacccggt
     4861 cgatggggtc cgcccgctca gttcaggcgg gtgatgcgcg ccgccggggg cgccagactc
     4921 gggtgggcct tcatgtagcc aacgaaagcg tcgatgtcga tcacgccgct gggagtgacg
     4981 ttcttgccgc tggcggtgac caccgagaag ttgtcgcccg gcccgggggc catgaagctg
     5041 ttgagggtga cgcgataggt cttggtgagg tccatcgcca cgccgttgag cttgagggag
     5101 ccggccacca cgcggttgcc ggaccccacc gccgcaccgg ccggcttgga ggcatcccag
     5161 gtgtagctga aggtgctgct cggctggagc atgcggccgc agccgttggc gccggtcttg
     5221 gcggcgcagt tgggggcttc ccactgctgc tccagcaggc ggacgaggtc ggcgccggtc
     5281 agatcgacgg tcaccagagt gttgccgaag ggcgccacgg tgagcagatc gccgtaggtg
     5341 acggcaccgc cgttcttgta gatcaggtcg gcgcgcacac cgcccggatt gatgaaggcg
     5401 atgtcggcct tggggccgcc ggccagatac acgtcggcca tcacgtcacc catggctcct
     5461 tcggcggttt cgtcgcgggt cggcgttgag ctgctggaga gcagggcgcg cttgatatcg
     5521 gcagtgatgg agccgaccac ctggttcttc agcgtggccg acaggctcac gtaggactgc
     5581 accgcggcgt cgatggtggc gtccttggtg aggatggaga agccggccgg caccgtggtg
     5641 ttgaggtcgt tgatgaccgg ccgggtgttg gcgtcgcggg tcagcacgcc gccgccgggc
     5701 agcagggtca ggttgatctc ggtgaccgcg ctgccataga agccggtgga ggtgagcagt
     5761 ttgccattgc gggtgcacac gtattcctgg tgggtgtgac cgctcaccac cacatccaca
     5821 ttcgaactca aggcatcgac gatgggcagg atgtcgccag tgaggttcgg gcaggtcttg
     5881 tcgttgaggg tcgaggcggt ggtctggccg ccctggtgga tcagcaccac caccgcattc
     5941 acgccgctgg ccttgaggct ggccgcgtac ttgttgatgg tggtcgcttc atcctggaag
     6001 tccagcccgg ccacgccggt ggaggtcacc acctggggcg tgcccttgag agtcaggccg
     6061 atgaaaccca cggacacggg gccgaacttc ttcacataag tggccggaaa cagcgattgg
     6121 ccggaactgc tgtccaccac gttggccgac agccatttgt aggtggcacc gctgaagctg
     6181 ccgcccttga tgcaggtatc ctggccgatg gtgccgccgg ggtagcagcc gccattctgc
     6241 aggcgcagca gctcggtctt gccgtggtcg aactcgtggt tgcccacgga ggtcacttcc
     6301 aggccgatct ggttgaggat gtccaccgtg gcctcgtcgt gggtcagggt ggaggtaacc
     6361 ggcgaggccc ccaccatgtc gccggcgccg acgacgatgc tgtaggggtt ctgggctttg
     6421 agctgcttga tcagggtggc caggtaggcc gcaccgccgg aacgcaccgt ggtgccggcc
     6481 gcgttggccg ggtccttgag caccaccgag ccgccgttgt tggccgccgg cacctcgatg
     6541 ttgccgtgga agtcgttcat ggcgatcagc ttgacgctca ccggcgccgc cgattcactg
     6601 ctgctggcgc cgccgccgca gcccgtcacc gcccacgcgg tgctcagggc tgccagccag
     6661 gcggccatct tcgtgctgac aggacgcttc atttcgatgc ctcggtcggt gcgaaaaata
     6721 aacccgcacg ctaccggccc aatgtgaaac gcctgtgaag ccccctcaga ggagcagatt
     6781 caccccgtgc agcagaatcc ggcaggccac cgcaatgccc agcgccactg ccaccgacaa
     6841 gcccagggag aacaccgcct cacgccgccc gacggtcttc agcatgaccg cgaaggtgga
     6901 cacgcagggc acgtagaaag tgatgaagac caggaaggtg gcgatctgca cccagtccag
     6961 caggggcgcg atctcctggg tgccgagggc ctggtagacc atcagcaggg acagctcctt
     7021 gcgcagcacg ccgaacagga tcggcactcc cagcaccacc ggcaggccca ggctccaact
     7081 ggtcaggggc accagcaggg tgttgatgac ctcgtccgcg ccaaaatggg tcagcagcgc
     7141 cagcaccacg ctgcccagca ccagcagcgg cgtgacaatg gtgaggatgt cgctggtgcg
     7201 ttcccaggtc accgccagca ccttgcgcca gcggggcagg gcgtaagccg gaatcgcctg
     7261 gatcatcccg gggctggcct gcacgtaacg gcgggtaagc agacgcccgc cgaccgcgat
     7321 gaccagcatg gtcagcagga agatgcccag caccccctcc cagcccagat acttgccacc
     7381 gatggccagc acgatggccg agcgcgccga gcagggcacg aaggtgatca gcagcgaggc
     7441 cacgatgcgc tcgcgccccg ccgagctggc cgccaccgcc gatagggccg gcacgttgca
     7501 ccccagaccg agcaggaagg gcaccgccac gccgccatgc aggccgatgc ggtggaaacc
     7561 gcggtccacc acgaaggcca cccggtgcat gatgccggac tcttccagcc ccaccagcag
     7621 cagcaccagc ggcagcatgt agggcaccac gatgcccacc agccccacca gcccgtcggc
     7681 gatggcgcga ccgatcaccc cggcggtagt gtccggctgc cattccccga cccacgccgc
     7741 cagcggcgcc gaggtaacgg aatcgagccc ggtgctcacc tcgaagacca cgtagagcac
     7801 cagggcgaac accgccaggg tgccgatcag gccccagcgc ggatgcagga agagccgatc
     7861 aagctggcgc ttccagccgg ccttctcggc cgcgtggccg aggcgcgtga cctgctcgtg
     7921 gagcagcgcg gcgcggtagt ggcggtcggc aaacaattct tccgaaaggg aacggggcag
     7981 ctgcgcgccc gccgccttcc gcgccgccgc gatgcgcggc atcagggccg ggaagcgctc
     8041 ggcaaactcc cgcgccagca gggtgtccgc cttggccagc tgggtcagca gcagggcccg
     8101 cggcaccgga aaggcggcct ccacctcggg caggtcgagg accgcgtcaa acccgtgcaa
     8161 ggcctgacgc aggtgcgggc tggccggctg gggcacgggg cacaccgacc ggcgcacggc
     8221 ctccagggcc gcctcgaaga gggccgagat gcccttgccc atgtgggcca cggtgggcac
     8281 cacggtcacg ccgagggcct cggcgagggc cgctgtgttg atgtagatac ccttctcacg
     8341 ggcctcgtcg aggcggttga gggcgatcac caggggcttg cccagcaggc acagctcctg
     8401 actcagggcc aggtcgcggg tcagggccgt ggcgtccacc acatgtacga ggacgtcggg
     8461 tgcccggaag cccccttcac ccggctcgcc ctccagcagt gcggtgagca cccgccggtc
     8521 ggcttcgtcc agatcgagca gggtgcgcag gggcggcaga tccaccaccc cggcctgctc
     8581 caggcccaca ttgacgacgc acgcgcggta gcccagcgca ctcccgtcca gttgacccga
     8641 ctgcacggcc gtactcgatg ccgcatcgaa cagggtgctc ttgccggtcc gcggcaggcc
     8701 caccagggcc accaccggac ggcagcgtcc acgcagcaga cgcgagggaa cggcgacacg
     8761 gacctccggg ggcagggcgg cactcatgat ccgctccgcc cggccgtaaa tgccatcgaa
     8821 atatgcctgt caaccacccc gtaccaccgt accacgccgg tcatgatgcg acgccccacc
     8881 gttcaggagc gatcattata ttggtaataa gaattgttcg cacctataaa cacgcacgct
     8941 gcacagggtc tttgtgtagc acgtggcgcg cgcgctcaga cccgcaggcc ggcgcgagtc
     9001 atcagcagca gggcctgggc acggctggtg acgccgaggg agcgcaggat cgccgagacg
     9061 tgcaccttga cggtgccttc ggacaggccg agcagatcgg cgatatcccg gttggacttg
     9121 ccctgggcca ccaactccag cacccgcccc tgggcggcgg tgagctggaa acgctcggca
     9181 aaggcctggc tggcccgccg cgtacggccg ctgccgtccg aacccgacgt ggcctctggc
     9241 gtgaacacgc caccgtccag caccgtccgc acggcctcga tgagcgtgtt gctggggctg
     9301 gccttggaga cgaagcccga agctccggcc ttcatggcgc ggtgcacgga ctcccggtcg
     9361 tccagggccg acaccaccag cgccgggaca tcggggaagc gcttggcgat gacaccgagc
     9421 agggagaagc cattcatctc cggcagcatg aggtccacca ccagcagatc gcagtcggga
     9481 tcctcctcca gcagggcaat ggcctcggcg gcgctgctgg cctgctggca gaagacgcct
     9541 tcgcccacct gggcgagggt cagagccatg gcttcccgga cgaggacgtg atcttcaatg
     9601 atcagaatac gggtcggcat catggcaaac gggcgctgga atcgcggcag aacctgaggt
     9661 caggccagtt taacctaccc tctggagggg aaaaaataga aaatccgggc cttccctgcc
     9721 aaagaaatca ataggaccca tcatgccgcg tattgccttt gccccgttca acgcagccgg
     9781 cctcctgacc agcctgtttt ccctgctcac cccgtcggcg gccggcgccg cccccaacgg
     9841 cgaggacagc ttcctggacg acctgccggt ggtcctgtca gccacccggc tgacccaggc
     9901 gccagccgac gcgccgggtt cggtgacggt cctggaccgg gacatgatcc gcgcgtcggg
     9961 ggcccgcaac atcagcgagc tgttccgcct ggtgcccggc ttccaggtgg ccatgcagac
    10021 cggcaacacg ccccaggtca cctatcacgg gctctcggac gaagcgccgc ggcgcatgct
    10081 ggtgcaggtg gatgggcgct ccgtgtattc cgcctatttc atctccggcg tggaatggaa
    10141 ccagatcggc gtggatctgg acgacatcga gcgcatcgag gtctttcgcg gttccaactc
    10201 cgccgcctac ggcagcaacg ccttcctggg cgtggccaac atcatcacgc gccccgcggc
    10261 cgagacccag ggcgtcgcac tgcgcctgcg cgggggaagc gatggcgtga acgatcaggg
    10321 cgtgcgcatc ggtcagcgcc taggtgacgt ggatctgcgc gtgaccgctg cacgcagcca
    10381 cgacaacggc tttgccgacc tcaatgactg gcgccgcaac gaactggcca acctgcgcgc
    10441 cgactggcac atccagcacg atcaccaggt ggaattccag gccggctgga cccgcaacga
    10501 gttcggcacc ggcaaggcca acaacctctc cgatccggag cgcagcgccg aactgtccac
    10561 aggcttcggc ctgctgcgct ggcgccacac gccccagccg gacgaggaac tgtcggtcac
    10621 gtattaccac cagcaggaga ccggcaagga cgcctatccc tacaccgtca tcgtcccgcc
    10681 tcacctcacc ggcctgccgg tgaatctgca gatccccttc ttcttcgaat acggctacaa
    10741 cgtcacccgc gacgatctgg aagcgcagcg catcaccagc ctgtcaccga cgctgcgtgc
    10801 cgtctatggc ggcggcctgc gctccgaccg cagccatgcg cccctgcgct tcaatacggc
    10861 cgacaccgtg cgcagcgaca cccggcacct gttcggcacc ctcgaatggc gcgcctctcc
    10921 ggcctggctc ttcaacctgg gcgcgatgct ggagaacagc tccctcaccg gcacctcggt
    10981 ggatccgcgg atctccgcca actaccattt cagcgacatc tccacctggc gcgcgtccat
    11041 caaccgctcc caccgtcacc ccagcgcctt cgaacagaag gccaacatgg tcttccgcaa
    11101 ttcggccgcc ttccccaccc ccatcggggc cgtgccggcg ggcaccgtga tctcccagac
    11161 cttccgcccc tcgccggaca tcaccgcgga acgcatcacc acctacgaga tcggctacct
    11221 cgccgagttg cgttccctcg ccaccacgat cgacgctcgg gccttcgtgg agcgggcacg
    11281 caacctcatc gagatgaaca tcgagaactc caccatcggc ctcgtcccgc gggacaacgt
    11341 ccgctacttc gccaacacgg gcgaagccga catccgtggc gtcgaactgt ccggcacctg
    11401 gcgtcccgcc gcccagacct gggtcaacct gacccacacc gaactgcgca tcgccggccc
    11461 caccgtcacc gccgcccagg ccgcccgcca gtccacgccc tacgtcagca acaccgcgcc
    11521 caagcacacc tccacggtct tcgcagcctg ggagttcgtg ccccgctggc aggtcagcgc
    11581 cagcaagcgc tgggtcggct cgatgagctg gtaccaggac gacccccacg cgatccccat
    11641 gtatcgccag ctcgacgtgc gcatcgccca ccgcttccag accgcctacg gccgcggcga
    11701 ggtcgccctg gtcggccaga acatcgacgg cggcgaacag accttcacgt ccaccgccgg
    11761 cgtgtggggc agccggacct tcgccaccct cagcctggag ttgtagtcgc cccttcatgg
    11821 gatgcacgat ccgccgcttc ctgctgatcc tgctgaccct cgccggtctg gcctcccctg
    11881 cccgggcggc gctggtggca ctggtgagcg ccgatgaagg cgccgccttc agcgaagccg
    11941 ccgacgcgat cagcgccgaa ctgcggggcg ccggccatcg cgtccagagc ttcacgctgc
    12001 ccctccgcgc ggacgacacg ggcttcgcct ccgcggcgct gatcgtcacc cttggcagcc
    12061 gtgccgccca gctcgtcagc accctgcccc agcacggccc ggtgctccac acgctgctgc
    12121 cgcgcagcgc cttcgaccgc ctgccgcggc ccgacgaccc ccgccgcgtc tccgccgtct
    12181 atatcgacca gcccgccggc cgccagctcg aactgatccg ccaggctctg cccgactggc
    12241 agcgcctggc cgtcatcacc ggccgcgaca gcgccgaact cggcacccgt ctgcaggccg
    12301 ccgcccgcga acatcggtcc ctgcgcgtgc agaccgacat cgccctcggc gagcgcgaca
    12361 tctaccctgt cctgcagcgc atgctggccg agccggccat cctggtcgcc gtgccggacg
    12421 ccaccctgtt caacagcaac acgatctcca acatcctcct cacggcctac caccaccgct
    12481 cgccggtggt cggcttttct gccgcctaca tcaaggccgg tgccctcctg gccctctaca
    12541 gcacgccggc ccagatcggc cagcaggccg gcgaagtggc ccgccaggcg ctggccagcg
    12601 gcaccctccc gccgccccag tacccgcggc agttccgcgt cggcaccaat gcctacgtgg
    12661 cccgctccct cggcatcacg ctcgaagacc ccaacgcctt gcgtgatcgc ctcgaacgca
    12721 cggagggcac gccatgagcg cccgcgatct cgacatcggc acccgggccc tggtgatcgc
    12781 ggtgctgccc gctttcgtcg tcgcggtggt gctgatcgcc tatttcaccc acagccgcct
    12841 caacgacctg gaagacgccc acagccagcg cggcaaggcc ctcgcccggc agctggccgc
    12901 ggcgagctcc tacgtggtat tcaccggcaa caaggacgcc ctcggcaagc tcgccgccga
    12961 aatccttgac gaacccgatg tcgtccgggt cgccgtgcgc ggccccggca aggacaccct
    13021 gaccgatctg cgcaagcccg ccccctccga cagcggcgcc ctgcgcctcg gcaacgccga
    13081 ctccgacaca ctcgccttcc aggaagccat ccacagcccc ggccagctct tcgacgaccc
    13141 cttcgtcggc gccgagggcg ccggcagcga gctccacgcc cagcagggcg aagtcatcgt
    13201 cgaactgtcc cgggacgccc tccacggcga agagacccgc ctgctgcgca atgccgtgct
    13261 gatggtgttc gcggtgctgc tgggcagcgt ggtcctcgcc ctgcgcctga gccgcggcat
    13321 cacccgaccg atccagcgca tcgcctccac cgtggcccgc atcggccagg gcgaacgtgc
    13381 cgcccgggtg cgcgtcgaag gcggccacac cctgcgcgcc cttggtcagg gcgtgaacga
    13441 catggccgac cgcatcgccg ctgccagcga gaatctggag cgccagatcg aagccgccac
    13501 cgccgaactg cttgaaaaga aaaacgaggc cgaactggcc aaccaggcca agacccgctt
    13561 ccttgccgcc gccagccacg acctgcgcca gcccatgcac gccctcggcc tgttcgtcgc
    13621 cgaactgggc cagaagcccc acgcccccga cacctgcacg ctggtggagc agatcgccat
    13681 gtccgccgag acgatggaag acctcctcga cagcctgctc gacatctccc gcctcgatgc
    13741 cggcgcactc gaaccccaga tcaagccctt cccgctgcag ccgctcctgg agcgggtgga
    13801 cgtggactac cgccgcgaag cggaacggcg cggccagcgc ctgcgcctgc gccccaccga
    13861 tctgtgggtc agcagcgacc cgctgctgct ggaacgcatc ctccacaacc tcatcagcaa
    13921 cgccctgcgt tatgcgccgg gcggcaccgt gctggtggcc tgccggcgcc gcggcgccca
    13981 ggtgaccgtg gaagtgcgcg acaacggccc gggcatcgcc ccggacgccc aggaggccat
    14041 ctttcacgag ttcgtccagc tcgacaatcc cgagcgcaac cgtgccaagg gcctgggtct
    14101 cggcctcgcc atcgtgcgcc gtctcaccac cctgctcggt caccccctcg gcctgcgctc
    14161 caggcccggc cgtggcgcac tgttctcggt caccctgccg gtcaccgcgc cccggcctca
    14221 ggacctcagc ccgccatcgt ccgcggcgcg gggcctcgcc ggcctgcatg tggccctcat
    14281 cgacgacgac cccctcgctg ccgccagcac cctgagcctg ctcgaatcct ggggctgcct
    14341 ggtcaccgcg gccgataccc tggagcacat gtacgccgcc ctgcagcgca gcgagatgag
    14401 cccggacatc atgctctgcg accaccacct ggacggcgcc ctcgacggcg tcgcggtggt
    14461 cgcccgcctc cggcagctcc tcggcaccga tctgaccgcc atcatcctga gcagcgacac
    14521 cacccttgcc gactcgcccc gcgtccgcga caacggcatc cgcgtcctgc gcaagccggt
    14581 ccgcccggcc cgcctgcgcg ccttgctgca acgcaagacg ggcagctgat gcagcgtccg
    14641 cacgcatcgg ttaagatgcc cgcaccacga caaccacgta caagaggggg agccaccatg
    14701 agcgccgaca acaccatgca ggacccgatg gaattcatgc gcaacatgtg gagcaagatg
    14761 ggcttcagcc tgcccggcat ggtcacgcca accctcgacc tggaagagct ggaccgccgc
    14821 atcaacgaca tgaagaccgt cgagaactgg ctcaagatga acctcagctc cctgcagatg
    14881 gccacccagg gcctcgagat gcagcgcgcc accctggccg ccatgcaggc catgagcaag
    14941 gtcgccaccg aaggcgccgg caaggtcgac gagggcaccc ccaacccctt cgccagcacc
    15001 atgtggccgt ggaacttcgt tccggccgcg gccgaagccg ccaaggccgc tgcggccccg
    15061 gccgagagcg ccgagccggc cgccaagccc gccgccaaga gcaagagcgc caagtaagtc
    15121 cgccgtccgc gtgcgcccgg cgccgtgccg ggtgccgccc ctcatcccgc ggccacaccc
    15181 cgcagggccg tcagccagcc cgtcagcgtc accgccgccg ccaccgtcgt ggccgagatc
    15241 agccaggcca ctccgggccc gtcaccgccc atgcgcatcg ccagaatgta ggccgacgat
    15301 gccgtcggca gggccgcgaa cagcaccgcc gtcgcaaaag ccagcccttc cagcccgaag
    15361 gcccccgcca gcccccacgc caccgccggc agggcgagca gcttcaccgc gcacagatag
    15421 accatgccca caggccggcc ctcccgctga ccgatccgca gcgccgcacc caccgcgatc
    15481 agacccaggg tcaccgccgc atccgccagc cgttgcagga agggcagcac cggccccggc
    15541 agatgcagcc cggcggtgct gaaggccagc cccgccagcg tcgcgatcac cagcggattg
    15601 cgcgccagtt cccgtagcag ccggccctgg ccgtggtggg ccaggaagcc caccgccgcc
    15661 atgttcgcaa agggcaccat cgcgccgcac agcgccccca tcgccgccac acctgccgcg
    15721 ccgtggaggg tgcccgccac cgcgatgccg atgtaggtgt tgaagcggta ggcgcactgc
    15781 acccgcgacg caaaggccat ctcccccaat cccatcagcg gccgcccgag gagcgccagc
    15841 gcgaagccgg acgccatcgt cagcaacccg accgcgaaca gcggcgccgc cgccgacagg
    15901 tcgatgggcg cccgcgccag ggcatggaac agcagggccg ggaagaacac gaaatacacc
    15961 agcttctcca ccccggccca gaagccgtcc tccagccgcc cgaagcggcg cagcaccacg
    16021 cccagggcga tcagggcgaa atccgggagg agcagcagga agctttgcat gggcgcacgg
    16081 acggggcgga gacgggtccg gcgagtgtgc cagaagccgg actgccggcg aaatccggac
    16141 cgtccgcaca cgccgcgcgc cgcacggcgt tccggggcac cgcacaggat ctgcccgagc
    16201 gcctgcgccg ctcacgccag cgctgaccgc cggacgctgc ggacgcgcgt cctgccggcg
    16261 tttacgcgta gcgccgcacg tcgtcgatca ccttgccgtc gttgggcagg ctgcccggcg
    16321 cgcagcactg cacctcgccg cgcagcttgg tgagctcccg caggctcgcg accaccgcct
    16381 ctgccagccc ggccccgtcg ccggccactt cgcagtggag cgtcatgcgg tcgttcagat
    16441 ccgggttgtc caccaccaga cggccgcgca ggatctccgg atggcgcttg agcaccgccg
    16501 ccacctggcc gggatggacg aacatgccct tcaccttggt ggtctgatcc gcccgtccga
    16561 gccagccgcg gatgcgcgca ttggtccggc cgcagggcga cgcgccgggc aggatcgccg
    16621 acaggtcgcc ggtgccgaag cggatcagcg ggtaatccgg gttgaaggtg gtcaccacca
    16681 cctcgcccac ctcgcccggc gccaccggat cgccggtgcc ggggcggacg atctcgacga
    16741 tcacgtcctc gtccaccacc atgccctccc gcgccggggt ctcgtaggcg accagcccga
    16801 ggtccgccgt ggcgtaggcc tggaaggcct ggatgccggc cgcggcgagg gcgtcgcgct
    16861 ggctgggcgg aaaggcctcg ccggagacga aggccttctt cagactgtcc aggcgcaggc
    16921 cctgctcggc cgccttgtcc agcaggatgc gcaggaagga cggcgtgccg gcgtacgcgt
    16981 cggggcgcag gtccagcatc gccgcgagct gctgctcggt ctgcccgacc ccggcgggga
    17041 acaccgtgca gcccagggcg tgggccgccg tctccatcat cgaaccggcc ggcgtgaagt
    17101 ggtaggagaa ggtgttgtgc accaggtcgc ccgcccggaa gcccgccgcg tagaaggccc
    17161 gtgccaggcg ccagtaatcc ttgcgcgcgc cctccggctc gtagatcggc cccggcgagg
    17221 cgaacacccg cgcacagccg gcgccccaac ccagcgccgc caggcccccg aagggccgcg
    17281 ccgccttctg cagcgccgac agctcggact tgcggatcac cggcaggctt gccagcgcct
    17341 cacgggacgt gaccgccgcc gcgtccacct ccgccagcag gcgcgcaaag gccggcgtac
    17401 gcgcctgggc gtgggccacc tggaccggca ggcgggcaag gagatcgcgc tcccgctcgg
    17461 cgggatcgcg ggtttcacgg gcgtcgtagt agttcatggg gtctccgcgt acttctggtt
    17521 ttatctcgtc catgcactgg acggctcatc ctgttttcgt cctaccgttg gcggggtctc
    17581 gcccccgcgg gcgacctcct ttcttgcttc gccaagaaag gaggcaaaga aggcgacccg
    17641 acctcggcgg tcggccttcg gccgactccc ctgcgctgct cccgtcaggc ggccggcgca
    17701 gaactcgccc tgcgggctca gacagctgcg ccggacttcc ccgcctgccg ctgcgctgct
    17761 cggcgcctca gacgggctgt ggccacccac ctcgccccgc cggatcgcat cgccatctgc
    17821 aatcgagaac acccggaccc cgttgggaaa acacccactt ccatctcgca cacgcttcga
    17881 cgcattcaat cctgtctccc gcatccacgc tccccatcac gtccagccaa ggcgcggtgt
    17941 gtccccaacg cccgtctgaa ccgccgagca gcacagaagc gggcggggaa gtccggcgcg
    18001 gctgtctgag ccggcaaagc cggcgagttc cgcgccggcc gcccgattcg agcagcgcag
    18061 gggagtcgtc cgcaccgcgg acgaccggtg aggtcgggtc gccttctttg cctactttct
    18121 tggcgaagca agaaagtagg tcggccgccg ggccgaactc ccggccaacg gtaggccacc
    18181 tccttacgac acgccccaaa cgatcaatgg aaccaaacaa cgcacgaccg cgttcgcacg
    18241 cccctcacaa cgagcgccgt tccgactttc cagcgccacc gcgccgcggc gggtccccgc
    18301 caacgcgtcc ccctcacgac agccaccgct tccgccgccg gtaatgcttc acgtcccgga
    18361 agc
//
LOCUS       DAOQPA010000072        18286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1532_length_18286_cov_18.849542, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000072 DAOQPA010000000
VERSION     DAOQPA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18286
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_18286_cov_18.849542"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PKX01_10910"
     CDS             complement(<1..1249)
                     /locus_tag="PKX01_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HMZ76681.1"
                     /translation="MNRLCYRIVFNRLRGMLMAVAECACARSRSSGQGRRPVPQQPPP
                     PASVAARLRPTVLRCWLAFGLVLPALPAPAQIVAYKNPPAGQTPTVLAAPNGVPLVNI
                     RTPNAAGLSHNAYSRFDVPPQGTLLNNSPDAVQTQLGGWVQGNPWLATGSARIILNEV
                     FSNDPSLLRGYIEVAGQQAQVVVANPSGVTCTGCGFIHAHRVTLTTGTPRVAADGRLD
                     AYRVTGGTVLIDGNGLDTSSSDFTEIIARAVRVNAGLWARQLHVAAGTSLVDAATLDT
                     TALASPTPAPTLAIDVASLGGMYARKITLVGTEAGVGVRSAGTLGASIGEFVISAAGH
                     LENTGTLTSADALRVTTTGLRNSGTFYAQGEARVSTTGVLENDGLIASAAHTAIAAAR
                     FIGTSGSVIASGLASDGSLGATGD"
     gene            complement(1295..3058)
                     /locus_tag="PKX01_10915"
     CDS             complement(1295..3058)
                     /locus_tag="PKX01_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HMZ76682.1"
                     /translation="MTATAPSARPFRCPFPRLLRLACALLLMVSTSDLLAQEDAAALE
                     FHRQQERERQLRQQQEPRPDVRLQDVSPSSARLPRDEHPCFPIRRIVLTGVEAERFQW
                     LADHTGRAEDGHPDPAIGACLGTGGVNLVMRRLQNALIERGYITTRILAAPQDLSTGT
                     LHLTVVPGRIHAVRFLPDASGQMPSRATHWNALPAAADDLLNLRDIEQALENFKRLPS
                     VEADLQIAPSEDAAATPGDSDLLITWQQTFPFRLTLSADDSGTQASGRHQGAATVAFD
                     NWWTLNDLFYITHSQSLFTDGTVRGTRGYTVHYSLPLGYSLLALTTSHNTYHQTVAGL
                     NGAVRYDGVNDTGDLRLSHILHRTAAGKTHVFLRGWQRASSNFTDDEEVLVQRRRMGG
                     WEAGAGHRAFIGQATLEGTVAYRRGTGAFDALPAPEEARGAMAEGTARPRLWTADLQF
                     NQPFRVGREAFRYGTSLRAQWNGTPLVPQDRFAIGGRYTVRGFDGELVLAAERGWFVR
                     NDLGWQIGGQELYLGLDHGAVAGRSAAQLLGRTLTGAVIGLRGGGSALSYDLFTGTPV
                     ARPEGFQAARTVAGFSITWTL"
     gene            3323..4987
                     /locus_tag="PKX01_10920"
     CDS             3323..4987
                     /locus_tag="PKX01_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76683.1"
                     /translation="MSIRRHILVLTSIAIVLLVAMAAAGGGALQRNLALIHTLTEEAV
                     PGVLIASDLDADLRQVQLDLLEVVRAPSGEVATPQRERLAASRTHMDALLQALALRSR
                     STVETGLLGQMRDSLVQYGQAMDETLALVDRGQRALAEANLFANVSEYERELHETLQT
                     FRVEKQRDKDASLQAVEVGMEETMIGLSGLGGLTLVALAVMAVRLHRRIVRPLRAMET
                     TMASIAGSLDFTQRVPVVRDDEIGQSVRAFNALLDTLQRSLGEMIAVIRNNTVATVEM
                     HQSAATVARIATQGSVSSRAIHAAVQSIREHILHIDQETRQAGTITEASAETAVRRSE
                     FVGATASRIAQLAARIDTASAKVFCLADEVMHIEGVVSEIRQIADQTNLLALNAAIEA
                     ARAGEAGRGFAVVADEVRKLAERSATATETINARIGAVLVASREATDLMTVVVGDMRE
                     STALAATASEAIADVRTSTGQVIDAVGEINRLVHIGHASSDEILSRMDHINGLLDQAT
                     CAAGHAQSAADAVRGISHAMAQIVDRFKIGEGSAVVAADAGAVQLF"
     gene            complement(4996..5730)
                     /locus_tag="PKX01_10925"
     CDS             complement(4996..5730)
                     /locus_tag="PKX01_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76684.1"
                     /translation="MNSPDKDSPLIFVVEDEADIARLICATLGEYGFRSESFATGRSL
                     LARVRRQAPELAILDLGLPDMDGLQVLRELQELHPCAALILTGRDGLTDRVLGLELGA
                     DDYLVKPFEPRELVARVRSILRRYQRAASAEPARHTGARFAGWTFDIGRLSLLHDDGR
                     ELSLSAAEAALLQTLLRRPNKILTREQLIGERDLDPFDRSIDVRISRLRRKLEDEPQD
                     PKIIKTVYGAGYLLCATVEWLGPGGA"
     gene            complement(5727..7787)
                     /locus_tag="PKX01_10930"
     CDS             complement(5727..7787)
                     /locus_tag="PKX01_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HMZ76685.1"
                     /translation="MIGRIAPGARAAAGSPEELRRYDLLLAGFDLLDQAIAVFDAAPR
                     LVTWNTAMLRLLDFPEELVRVGTPFEDFVRFNVERGEYGPGETERLIAERMAAVRAFQ
                     PHQAERTRPNGQILAIRGVPIPNLGFVTLWTDVTEQRRYEKLIEGQNAELETRVRART
                     AELEAANRDLTEAKSRLDSVAAQLSRSEERLRLIMDAIPALIAYVDAGERYRFANRGY
                     AQWFGLDKNAIVGRSIAEVFGPDAYAQIKPHLETAADGERVSYEYTRTDASGRQVFAR
                     SAVVPDTSEAGGVQGYFVLSIDITEQKASQAALIQAQKMEAVGQLTGGLAHDFNNLLT
                     IITGNLHALRDKLPAHQGYDDYLEPALSAAGRGTELIRRLLSFSRQQPLEPRAIEVGV
                     LVTGMGQLLSRTLTEHIRLQLDLPTRKLHVLADPHQLENALLNLALNARDAMPDGGEL
                     RIAVAPRHISASLSLLVEVPAGEYVQFDVSDTGTGIDPALLPRLFEPFFTTKRFGAGS
                     GLGLAMVYGFVRQSGGNIRILSTPGKGTNVRFILPMTEAPAPRSDQPPARAAHHDAPR
                     GLVLLVEDEPEVRKVIREQLTELGYPVLEAANGIEAAAMLEAVDDIAVLVSDTVMPGL
                     GGRELAARARRLRPQLPILLITGYATQAPADGPDLPTLRKPFDRSDLAAALDALHRPE
                     PAAP"
     gene            complement(7784..9895)
                     /locus_tag="PKX01_10935"
     CDS             complement(7784..9895)
                     /locus_tag="PKX01_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76686.1"
                     /translation="MSDLLPEHLKAEAVQLLEGLLANALIGIYLIQDGLFRHVNSQFC
                     ETFGYAAAEICDRLGPEDLVFPDDVERVRQTLADRIEGRQAAARYTFHGRHRSGRQLV
                     VEVFGLRIDVAGRPAVVGVLSDITARHVAERATAEQLGFIAQLIEAIPSPLFFKDAEA
                     RYLGCNQAFERFIGMAREAIIGKSVFEISPPDLAVRYHAADKALFDNPGVQTYEASVE
                     SSDGGRRDVLFNKATFLRGDGSMGGLVGVITDITERKRTEALIWAQANYDPLTGLPNR
                     RLLNDRLIEHVKRADRDGSSVALLFIDLDRFKEVNDSLGHAAGDLLLEEAAHRILACV
                     RETDTVARQGGDEFAVLLPGLHEHGPIERIAEAILRILGEPFAVGADECFVSASIGIT
                     LYPRDATSPADLFKNADQAMYHAKDNGRSRFSYFSPALQEAALHHLQLGRDLRQAIAG
                     HQLRVEYQPIYHLASGALLKAEALLRWTHPTRGEIGPAEFIPTAEEIGLIDEIGDWVF
                     ARVTETLLRWRATLAPGDAERCPLQITVNTSPRQFTGGRSVEAQLDRMDAAGLPGACI
                     ALEITESLLLGAQPRVADRLARLSARGVQVSLDDFGTGYSAMAYLKRLDIDTLKIDRS
                     FVRDLTTDSSDLAIAEAIIAMAHKLGLDVVAEGIETDEQRRILAATGCDFGQGYLFAR
                     PLSEDALLTLLEGQRRALATP"
     gene            complement(9936..11024)
                     /locus_tag="PKX01_10940"
     CDS             complement(9936..11024)
                     /locus_tag="PKX01_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ76687.1"
                     /translation="MPMSSSSAAPAPSPAAPERRRASVAMGFVTGMVAGMQRRGHDPA
                     PLLAATDIDLADSASRIPLDRYAALYNRVIRDLDDEGFGLFERPLGTGSFEFLCRGML
                     GAPHLAEALERARRFLHIVLPDLAVSIHRLDSHAELRIAETRPLCPTRDDPARVFAFE
                     WLLRLLHGLACWLVGRGLALDAVRFPFARPPHADDYQLIYTAHFSFGGDDLAARFNPT
                     LLDLPIRRDEAALARFLEGAPGKITTLYRRDRDTVVRVRDLLRAALPANLSQEHVADR
                     LHLSPRTLHRRLDEEGSSFRAIKEALRRDIAVARLTKTRHSIARVAADLGYADTSAFY
                     RAFIAWTGLSPERYRKQLAAQAPAQSAP"
     gene            11159..14425
                     /locus_tag="PKX01_10945"
     CDS             11159..14425
                     /locus_tag="PKX01_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ76688.1"
                     /translation="MTDLSVAKKLVTYKPKNKVRFVTAAALFDGHDASINIMRRILQS
                     TGCEVIHLGHNRSVGEIVNAALQEDVQGIAITSYQGGHVEFFKYMIDLLREHGGENIR
                     VFGGGGGVIVPAEIKELHDYGVTRIYSPEDGAILGLQGMINDVVERSDMDLSHAGPQG
                     ADAVLAALRSGDRRALARIITGLENGAYADEVKTALIAAAKELKTPTLGITGTGGAGK
                     SSLTDELVRRFRLDQHDRLKLAIVSIDPSRKRTGGALLGDRIRMNAIEHPNIFMRSLA
                     TRDTGKEVSVALPEVIAATKLAGFDLVIVETSGIGQGDAAIVPFVDVSLYVMTPEFGA
                     ASQLEKIDMLDFADFVAINKFDRKGAEDALRDVRKQVQRNRELFTTQVEQMPVFGTMA
                     ARFNDDGVTALYQALAPALVAKGLKLGKPGLPLVSVRASSRGRAIVPAERARYLAEIA
                     DAVRGYHKHTLEQSRIARERQALRISKRLFEGCQKDAGSFDELIDWKDGELTPSSRKL
                     LDMWPKTVELYAADEYVVKIRDKEIRTALTSESLSGSKIRKVALPSFEDDGESLRFLM
                     KENVPGSFPYTAGVFAFKREGEDPTRMFAGEGDAFRTNRRFKKVSEGMPAHRLSTAFD
                     SVTLYGCDPDPRPDIYGKVGNSGVSIATLDDMKVLYDGFDLVNPTTSVSMTINGPAPI
                     ILAMFFNTALAQQLEKFKAQNGREPTDDEAQKIREWVLATVRGTVQADILKEDQGQNT
                     CIFSTEFALKMMGDIQEFFVHNKVQNFYSVSISGYHIAEAGANPISQLAFTLSNGFTY
                     VESYLARGMHIDDFAPNLSFFFSNGMDPEYSVIGRVARRIWAVAMKNKYGANERSQKL
                     KYHIQTSGRSLHAQEMDFNDIRTTLQALIAIYDNCNSLHTNAYDEAITTPTEESVRRA
                     MAIQLIINREWGLAKNENPNQGAFIIEELTDLVEEAVLKEFEAIASRGGVLGAMETGY
                     QRGKIQEESLYYEHKKHDGSYPIIGVNTFLNPKGMAQQEIELARSSEDEKQSQLQRLA
                     AFQDRNSDEAPQWLAKLKQTVIENGNVFAVLVDAVKYCSLGQITTALYDVGGQYRRSM
                     "
     gene            14602..14829
                     /locus_tag="PKX01_10950"
     CDS             14602..14829
                     /locus_tag="PKX01_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ76689.1"
                     /translation="MHTTIGAGDFKARCLKLLDDVARSGEALVITKHGKPVAKLVPMP
                     PAGSLFGALAGSVRQEGDIVAPVDDDWDACR"
     gene            14826..15245
                     /locus_tag="PKX01_10955"
     CDS             14826..15245
                     /locus_tag="PKX01_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ76690.1"
                     /translation="MSPLLLDTHVLVWLLAGSDRLGLRARAAIDAAAAVEGVFVSAIT
                     PWEIAMLVAKGRLVLERDVGDWVQAALALPGIRLEALSPAVAVASTRLPGALHADPTD
                     RIIVATARHLGAALVTADGLLLDYASSGNLKVLPAGQ"
     gene            complement(15360..16457)
                     /locus_tag="PKX01_10960"
     CDS             complement(15360..16457)
                     /locus_tag="PKX01_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HMZ76691.1"
                     /translation="MSFRALPARALAAAGVVLATLVSPALQAAPPLPALNIDVNQTSV
                     SGISSGGFMSVQFQVAHSSIVAGAGVVAGGPYACAQGDTTRAVSQCSCTGKPFVACQV
                     TDHSADVPTLTDKVKTLAAQGAIDAPSHLARQRIVTVSGAKDTLVPPAIAQQLDAFYA
                     ALGVPAAQISPVSLPNAAHTMPTSSYGIACGKEDAPYIGKCGYDSAGAILQWIYGALT
                     PAGAKAAGSFSEFDQRRYVPEERRDYTGFKSGMDATGWLYVPNSCRKGERCRVHIVLH
                     GCKQGQSYMPLRPPPDGGLYNGTLFVKHTGYDRWADNNHLVILYPQALTVPGNPNGCW
                     DWWGYTGQDYATREGVQIRTLRAMVDALAGQ"
     gene            complement(16740..17456)
                     /locus_tag="PKX01_10965"
     CDS             complement(16740..17456)
                     /locus_tag="PKX01_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMZ76692.1"
                     /translation="MKLLLLLLSGLKFGKLLTTGGTMLLSVAVYAFVWGWRYAVGFVV
                     LLFCHEMGHYIAARQRGLHVGVPTFIPFVGAWIDLKEQPMNAETEAYVAMAGPLAGTL
                     ASVACYFLARSEGSDLLLAISYSGFFLNLFNLIPVPPLDGGRMTGVVSRRFWLLGAPV
                     LVGLFLWRPSPMLILVAILAAPHMLRAFRGEGGEAPGYYDTPAGKRFEYALLYLGLLA
                     YLAVMSYEVHEMLGAARGGM"
     gene            complement(17453..>18286)
                     /locus_tag="PKX01_10970"
     CDS             complement(17453..>18286)
                     /locus_tag="PKX01_10970"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HMZ76693.1"
                     /translation="ASSPAAATDPQARLAAAAQAAQAALQKGPRDIALAGQGVLKLPE
                     GFGFIPAGPAGEFLKAQGSRVGPDFQGLVVGDAIDGFVVVRFEDAGYVKDEEAREWDA
                     AKLLDNLKSGTEAANEDRRKLGMPEFSVQGWVEAPRYDEANHRLVWSASTREKNGAAS
                     DGDGVNYNTYVLGREGYFSMNLVTSLATVEQQKPVAQQLLAALSFDEGKRYADFNAST
                     DKVAEYGLAALVGGIAAKKLGLLATLGVFLAKFWKVAALAVVGAGAGLRKLFGRKDGQ
                     A"
BASE COUNT         2868 a         5945 c         6678 g         2795 t
ORIGIN      
        1 ggtcgcccgt cgcgccgagg cttccgtcgg acgccaggcc cgaagcgatg acgctacctg
       61 acgtcccgat gaagcgtgcg gccgcaatgg cggtgtgtgc ggctgaggca atgaggccat
      121 cgttctcgag gacgccggtc gtcgagacgc gggcctcgcc ctgcgcatag aacgtgccgc
      181 tgttacggag tcctgttgtc gtgacccgga gtgcatcggc ggatgtgagc gtgccggtgt
      241 tctcgaggtg tccggcggcg ctgatgacga actcgccgat gcttgcgccc agcgtgccgg
      301 cgctgcgtac gccgacgccg gcttcggtgc ccacgagggt gatcttgcgg gcgtacatcc
      361 cgcccaggct ggcgacatcg atggccaggg tgggggctgg cgtggggctg gcgagggccg
      421 ttgtgtctag agtggccgca tcgacgaggc tggtcccggc ggccacatgg agctgccgcg
      481 cccacagccc ggcgttgacc cgtactgcgc gggcaatgat ctcggtgaaa tcggagctcg
      541 atgtgtccag tccgttgcca tcgatgagga ccgtgccacc cgtcacgcgg tacgcgtcca
      601 gacgtccgtc cgcggcgacg cgcggggtgc ctgtcgtgag ggtcacgcgg tgtgcatgga
      661 tgaatccgca tcccgtgcag gtgacgcccg agggattggc gaccacgacc tgggcctgct
      721 gaccggccac ttcgatgtag ccccgcagca ggctggggtc attgctgaag acttcgttga
      781 ggatgatccg ggcgctgccg gttgccagcc aggggttgcc ttgcacccag ccgccaagct
      841 gggtctggac ggcatcgggg ctgttgttga ggagcgtgcc ctggggcggg acgtcgaaac
      901 ggctgtaggc gttgtgggac agaccggcgg cattgggcgt gcggatattg accagcggca
      961 cgccgttggg ggcggccagc accgtgggtg tctgaccggc agggggattc ttgtaggcga
     1021 cgatctgagc gggcgcgggg agcgcaggca gcacgagccc gaaggccagc cagcagcgca
     1081 ggacggtggg tcgcaggcgc gccgccacgg atgccggcgg tgggggctgc tgcggcaccg
     1141 ggcgccggcc ttgcccggac gaacggctgc gcgcgcaggc gcattcggcg acggccatca
     1201 gcatgccgcg cagacggttg aagacgatgc ggtaacacag tcggttcatg gtgggctccg
     1261 ggcgggggcg cgtatggggc gaagtggggg ggcgtcagag cgtccaggta atggagaacc
     1321 cggcgacggt ccgcgcggcc tggaagccct cgggtcgggc taccggcgtg ccggtgaaga
     1381 ggtcgtagga cagggcgctc ccgcccccgc gcaggccgat cactgcgccc gtgagggtgc
     1441 gtccgagcag ctgggcggcg ctgcggccgg cgactgcccc gtggtcgaga cccagataca
     1501 gttcctggcc gccgatctgc cagcccaggt cgttgcggac gaaccagccg cgttcggcgg
     1561 caagcacgag ttcgccgtcg aatcctcgca ccgtgtagcg cccgccgatg gcgaagcggt
     1621 cctggggtac gaggggggtt ccgttccact gcgccctgag ggacgtgccg tagcggaagg
     1681 cttcgcgacc cacgcggaag ggctggttga actgcaggtc ggcggtccac aggcgggggc
     1741 gcgcggtgcc ctctgccatg gcgccgcgcg cttcttccgg ggcgggcagg gcgtcgaagg
     1801 ctcccgtgcc gcgccggtag gccaccgtgc cttcgagtgt ggcctgaccg atgaaggccc
     1861 ggtggccggc gccggcttcc cagcccccca tgcgacggcg ttgtacgagc acctcttcgt
     1921 catccgtgaa gttgctggag gcgcgctgcc agccgcgcag gaaaacatgc gtcttgcctg
     1981 cggcggtgcg gtgcaggatg tggctgagac ggaggtcgcc ggtgtcgttg acgccgtcgt
     2041 aacggacagc gccgttgagg cccgcaacgg tctggtgata ggtgttgtgg ctggtggtca
     2101 gggccagcag gctgtagccg aggggcagcg aatagtggac ggtatagccg cgggtgccac
     2161 ggaccgtgcc atccgtgaag aggctctggg aatgggtgat gtagaagagg tcgttcagcg
     2221 tccaccaatt gtcgaaggcg acggtggctg cgccttgatg gcgtccactg gcctgggtgc
     2281 ccgaatcgtc ggcggagagt gtcagccgga aggggaaggt ctgctgccag gtgatcagca
     2341 ggtcgctgtc gcccggtgtc gcggcggcgt cctcggacgg cgcgatctgc aggtcggcct
     2401 cgacgctggg caggcgcttg aagttctcga gggcctgttc gatgtcgcgc aggttgagca
     2461 gatcgtctgc tgcggcggga agcgcgttcc agtgggtggc gcgggagggc atctggcctg
     2521 aggcgtccgg caggaagcgg acggcgtgga ttctgccggg gaccacggtg aggtgcaggg
     2581 tgccggtgga cagatcctgg ggcgcggcga ggatgcgggt ggtgatgtag ccgcgctcga
     2641 tgagggcgtt ctgcaggcgc cgcatgacga gattgacacc gccggtcccg aggcaggcgc
     2701 cgatggcggg atcgggatgg ccgtcctctg cgcgcccggt gtgatccgcc agccattgaa
     2761 agcgttcggc ctcgacgccc gtcaggacga tccggcggat cggaaagcac gggtgttcgt
     2821 cgcgggggag gcgcgccgag cttggtgaga cgtcctgcag gcgcacatcc gggcggggtt
     2881 cctgctgctg gcggagctgc cgttcgcgct cctgctgccg gtggaattca agggcggcgg
     2941 catcttcctg ggcgaggagg tcggaggtgc tgaccatgag caggagggcg caggccaggc
     3001 gcagcaggcg cggaaacggg caccggaagg ggcgtgcgct gggcgctgtc gcggtcatgg
     3061 catcagaggc aataggtgaa cattgttaca gtatatgtcc atggaaaatt gattttcaat
     3121 atgccaatag atatatgtct tatggaaagc gaggcaaaaa tgggtgttca gggggcgctt
     3181 cgagcggccc ggagaggacg cgagaggcgc tggcgtgcgc cttcacaaat aattcatttc
     3241 agcttgccgt cgggccgtcg atatccccgg cgcagtcgtg ttgcgctgcg tcatccgtca
     3301 tgtccgcccg gcaggtatcc ccatgagcat ccgtcgccac atcctcgtcc tgacctccat
     3361 cgccatcgtc ttgctggtcg ccatggcggc ggcaggcggc ggggcgctcc agcgcaacct
     3421 ggcgctgatt cacaccctga cggaggaggc ggtgcccggc gtgctcatcg catcggatct
     3481 ggacgcggac ctgcggcagg tccagctgga tctgctggag gtggtccgcg caccgtcggg
     3541 cgaggtcgcg accccgcagc gggagcgcct ggcggccagc cggacgcaca tggacgcctt
     3601 gctgcaggcg ctggcgctcc gctcccgttc gacggtggag accggcctgc tggggcagat
     3661 gcgggacagc ctggtgcagt acggtcaggc gatggacgag accctcgccc tggtggatcg
     3721 gggacagcgc gcgctggcgg aggcgaatct ctttgccaac gtgtcggaat acgagcgcga
     3781 gctgcacgag acgctgcaga ccttccgcgt ggaaaagcaa cgtgacaagg acgcctcgct
     3841 ccaggccgtg gaggtcggca tggaggagac gatgatcggc ctgagtgggc tgggcggact
     3901 gaccctggtg gcactggcgg tcatggcggt gcgcctgcac cggcgcatcg tgcggcccct
     3961 gcgggcgatg gagacgacga tggcgtcgat tgccggctcg ctggatttca cgcagcgcgt
     4021 gccggtggtc cgcgatgacg agatcgggca gtcggtgcgg gccttcaatg cgttgctgga
     4081 caccctgcag cgcagcctgg gtgagatgat cgcggtgatc cgcaacaaca cggtggccac
     4141 cgtggagatg caccagtcgg cggcgacggt ggcccggatc gcgacccagg ggagcgtttc
     4201 gtcgcgggcc atccacgcgg ccgtgcagtc gatccgcgag cacatcctgc acatcgacca
     4261 ggagacccgc caggcgggca cgatcaccga ggcctcagcc gagacggcgg tgcggcgcag
     4321 cgagttcgtc ggcgctacgg cgagccgcat cgcccagctg gcggcgcgca tcgacacggc
     4381 gtcggcgaag gtgttctgcc tcgccgacga ggtgatgcac atcgagggcg tggtgtcgga
     4441 gatccgccag atcgccgatc agaccaacct cctggccctc aacgcggcga tcgaggcggc
     4501 ccgcgcgggc gaggccgggc gcggctttgc cgtggtggcc gacgaggtgc gcaagctggc
     4561 ggagcgctcc gccaccgcca ccgagacgat caacgcgcgc atcggagcgg tactggtcgc
     4621 gtcgcgggag gcaaccgacc tgatgacggt cgtggtgggc gacatgcgcg agagcacggc
     4681 gctggcggcg acggcgagtg aggccattgc cgatgtgcgc acatccaccg gccaggtcat
     4741 cgacgcggtg ggcgagatca accggctggt gcacatcggg cacgcgtcga gcgatgagat
     4801 cctgagccgg atggaccaca tcaatggcct gctggatcag gccacctgcg ccgccgggca
     4861 cgcccagtcg gcagcggatg cggtgcgggg catctcccac gccatggcgc agatcgtcga
     4921 tcgcttcaag attggcgaag gctctgcggt ggtggctgcg gatgcagggg cggtgcagct
     4981 gttctgaagg cgggttcagg cgccgccggg ccccagccac tccaccgtgg cgcacagcag
     5041 atagccggcc ccatagacgg tcttgatgat cttcgggtcc tggggctcgt cctccagctt
     5101 gcggcgcagg cgcgagatgc gcacgtcgat gctgcggtca aaggggtcca ggtcgcgttc
     5161 gccgatgagc tgttcgcggg tgaggatctt gttggggcgg cgcagcaggg tctgcagcag
     5221 ggcggcttcg gcggcggaca gggacagttc gcggccgtcg tcgtggagca ggctgaggcg
     5281 gccgatgtcg aaggtccagc cggcgaagcg ggcgcccgtg tggcgggccg gttcggcgct
     5341 ggcggcgcgc tggtagcggc gcaggatgga gcgcacgcgg gccaccagct cgcgcggttc
     5401 gaagggcttg acgaggtagt cgtcggcgcc cagttcgagg cccagtaccc ggtcggtgag
     5461 gccgtcgcgg ccggtcagga tgagggctgc gcaggggtgg agttcctgca actcgcgcag
     5521 cacctgcagg ccgtccatgt ccggcaggcc caggtcgagg atggccagct cgggcgcctg
     5581 gcggcgcaca cgggcgagga ggctgcggcc ggtggcgaag gattcgctgc gaaaaccgta
     5641 ctcgccgagg gtggcgcaga tgaggcgggc gatgtcggct tcgtcttcga cgacgaagat
     5701 gagggggctg tccttgtcgg ggctgttcat ggggcggcgg gctccgggcg atgcagggcg
     5761 tcgagggcgg cggccaggtc cgaccggtcg aagggcttgc gcagggtcgg caggtcgggg
     5821 ccgtcggcgg gggcctgggt ggcgtaaccg gtgatcagga ggatgggcag ctgcgggcgc
     5881 aggcgtcgcg cccgggcggc gagttcgcgc ccgccgaggc ccggcatcac ggtgtcggag
     5941 accagcacgg cgatgtcgtc cacggcttcc agcatggcgg cggcttcgat cccgttggcg
     6001 gcttccagca ccgggtagcc cagttcggtg agctgttcgc ggatgacctt gcgcacctcg
     6061 ggttcatcct cgacgaggag gaccagtccg cgcggcgcgt catggtgggc ggcgcgggcg
     6121 gggggctggt cgctgcgcgg ggcgggcgcc tcggtcatgg gcaggatgaa gcgcacgttg
     6181 gtgcccttgc ccggcgtgct gaggatgcgg atgttgccgc cggactggcg cacgaagcca
     6241 tacaccatgg ccagcccgag gccgctgccg gcaccgaagc gcttggtggt gaagaagggc
     6301 tcgaagaggc gcggcagcag ggcgggatcg atgccggtgc cggtatccga gacgtcgaac
     6361 tggacgtatt cgccggccgg cacctccacc agcagggaca ggctggcgct gatgtggcgc
     6421 ggggccacgg cgatgcgcag ctcgccgccg tcgggcatgg cgtcgcgggc gttgagggcc
     6481 aggttgagga gcgcgttctc gagctggtgg ggatcggcca ggacgtggag cttgcgggtg
     6541 ggcaggtcga gctgcaggcg gatgtgttcg gtgagggtgc gggacaggag ctgacccatg
     6601 cccgtcacca ggacgcccac ctcgatggcg cgaggttcca ggggctgctg gcgggagaaa
     6661 ctgagcaggc ggcggatcag ctcggtgccg cgcccggcgg cggacagggc gggctcgaga
     6721 tagtcgtcgt agccctggtg ggccggcagc ttgtcgcgca gggcgtggag gttgccggtg
     6781 atgatggtga gcaggttgtt gaagtcgtgg gcgaggccgc cggtgagctg gccgacggct
     6841 tccatcttct gggcctggat gagggcggcc tgactggctt tctgctcggt gatgtcgatg
     6901 gacaggacga agtagccctg tacgccaccg gcctccgagg tgtcgggcac caccgcgctg
     6961 cgggcaaaga cctggcggcc gctggcgtcg gtgcgggtgt attcgtagct gacgcgctcg
     7021 ccatcggcgg cggtctcgag atggggcttg atctgggcgt aggcgtcggg cccgaagacc
     7081 tcggcgatgc tgcgcccgac gatggcgttc ttgtcgagtc cgaaccactg ggcgtagccc
     7141 cggttggcga agcggtagcg ctcgccggcg tccacgtagg cgatcagggc cgggatggcg
     7201 tccatgatga ggcgcaggcg ctcctcgctg cggctcagct gggcggcgac gctgtcgagg
     7261 cggctcttgg cctcggtgag gtcccggttg gcggcttcca gctcggcggt gcgggcgcgg
     7321 acgcgggtct ccagctcggc gttctggcct tcgatgagct tttcgtagcg gcgctgctcg
     7381 gtgacatcgg tccacagggt gacgaagccc aggttgggga tgggcacgcc gcggatggcc
     7441 aggatctgcc cgttgggccg ggtgcgctcg gcctggtggg gctggaaggc gcgcacggca
     7501 gccatgcgtt cggcgatgag gcgttcggtc tcgccggggc cgtactcgcc gcgctccacg
     7561 ttgaagcgca cgaagtcctc gaagggggtg ccgacgcgca ccagttcttc cgggaagtcc
     7621 agcaggcgca gcatcgcggt gttccaggtg accagacgcg gagcggcgtc gaagacggcg
     7681 atggcctggt ccaggaggtc gaagccggcg agcagcaggt cgtagcggcg cagttcctcc
     7741 ggcgagccgg cggcggcccg cgcgcctggg gcgatgcggc cgatcatggg gtggcgaggg
     7801 cccggcgctg gccttccagc agggtgagga gggcatcctc ggagagcggc cgggcaaaca
     7861 ggtaaccctg gccgaagtca caaccggtgg cggcgaggat gcggcgctgc tcgtcggttt
     7921 cgatgccctc ggcgaccacg tccaggccga gcttgtgggc catggcgatg atcgcctcgg
     7981 cgatggccag gtcgctggag tcggtggtga ggtcgcgcac gaaggagcgg tcgatcttga
     8041 gggtgtcgat gtccaggcgc ttcaggtagg ccatggccga gtagccggtg ccgaaatcat
     8101 ccagggagac ctgcacgccc cgcgcgctga ggcgggcgag gcggtcggcg acccgcggct
     8161 gggcgcccag cagcaggctc tcggtgatct ccagggcgat gcaggcgccg ggcaggccgg
     8221 ccgcgtccat gcggtcgagc tgggcttcca ccgagcggcc cccggtgaat tgccgcgggg
     8281 aggtgttgac ggtgatctgc agcgggcagc gttcggcgtc gccgggggcc agggtggcgc
     8341 gccagcgcag cagggtctcg gtgacgcggg cgaacaccca gtcgccgatc tcgtcaatga
     8401 ggccgatttc ctcggcggtg gggatgaact cggccggccc gatctcgccg cgggtcgggt
     8461 gggtccagcg cagcagggcc tcggccttga gcagggcgcc gctggccaga tggtagatgg
     8521 gctggtactc gacgcgcagc tggtggccgg cgatggcctg acgcaggtcg cggcccagtt
     8581 gcaggtgatg cagggctgcc tcctgcaggg ccggcgagaa gtagctgaag cggctgcgcc
     8641 cgttgtcctt ggcgtggtac atggcctggt cggcgttctt gaacaggtcg gcgggggagg
     8701 tggcgtcgcg cgggtagagg gtgatgccga tgctggccga tacgaagcat tcgtcggcgc
     8761 ccaccgcgaa gggctcaccc aggatgcgga ggatcgcctc ggcgatgcgt tcgatggggc
     8821 cgtgttcgtg caggcccggc agcagcaccg cgaattcgtc gccaccctgg cgcgccaccg
     8881 tgtcggtctc gcgcacgcag gccagaatgc ggtgggcggc ttcttcgagc agcaggtcgc
     8941 cggcggcgtg gccgagggag tcgttgacct ccttgaagcg atccaggtcg atgaacagca
     9001 gggccacgct gctgccgtcc cggtcggcgc gcttgacgtg ttcgatgagg cggtcgttga
     9061 gcagccgccg gttgggcagg ccggtgaggg ggtcgtagtt ggcctgggcc cagatcaggg
     9121 cttcggtgcg cttgcgctcg gtgatgtcgg tgatcacgcc caccaggccg cccatgctgc
     9181 cgtcgccgcg caggaaggtg gccttgttga agagcacgtc gcggcgcccg ccatcggagg
     9241 attcgacgct ggcctcgtag gtctgcacgc cgggattgtc gaacagggcc ttgtcggcgg
     9301 cgtggtagcg gacggccagg tcggggggcg agatctcgaa gacggacttg ccgatgatgg
     9361 cttcccgggc catcccgatg aagcgctcga aggcctggtt gcagcccagg tagcgggcct
     9421 cggcatcctt gaagaagagc gggctgggaa tcgcttcgat gagctgggcg atgaagccca
     9481 gctgttcggc ggtcgcgcgt tcggccacgt ggcgcgccgt gatgtccgag agcacgccca
     9541 ccacggccgg gcggccggcg acgtcgatgc gcagaccgaa gacttcgacg accagctggc
     9601 ggccgctgcg atggcgtccg tggaaggtgt agcgtgccgc agcctggcgg ccttcgatgc
     9661 ggtcggcgag ggtctggcgg acccgctcca cgtcgtccgg aaacaccaga tcctccgggc
     9721 cgaggcggtc gcagatctcc gccgccgcgt agccgaaggt ctcgcagaac tggctgttca
     9781 cgtggcggaa caggccgtcc tggatcaggt agatgccgat cagggcattc gccagcagtc
     9841 cctccagcaa ctggacggcc tctgctttca gatgctcggg cagcaaatcg gacatggggt
     9901 cgggcgcgcg gatgatcggt gcattgtaag ccgaatcacg gcgcactctg tgccggggcc
     9961 tgggcggcga gctgcttgcg gtagcgctcc ggcgacaggc cggtccacgc gatgaaggcc
    10021 cggtagaacg cggaagtgtc ggcgtagccc aggtcggcgg cgacgcgggc gatggagtgg
    10081 cgggtcttgg tcaggcgcgc cacggcgatg tcgcggcgca gggcctcctt gatggcgcgg
    10141 aagctggagc cctcctcgtc gaggcggcgg tgcagggtgc gcggcgacag gtgcaggcgg
    10201 tcggccacgt gctcctggga caggttggcc ggcagcgccg cgcggagcag gtcgcgcacc
    10261 cgcacgacgg tgtcccggtc gcggcggtag agggtggtga tcttgcccgg cgcgccttcc
    10321 aggaaacggg ccagggcggc ctcgtcgcgg cggatcggca gatccagcag ggtggggttg
    10381 aagcgcgccg ccagatcgtc gccgccgaag ctgaagtggg cggtgtagat cagctggtag
    10441 tcgtcggcgt ggggcgggcg ggcgaagggg aagcgcaccg catccagcgc caggccgcgc
    10501 cccaccagcc agcaggcgag gccgtggagc aggcgcagca gccactcgaa ggcgaagacc
    10561 cgcgccgggt cgtcccgcgt cgggcacagg gggcgggtct cggcgatgcg cagctcggcg
    10621 tggctgtcga ggcggtggat gctcaccgcc agatccggca gcacgatgtg caggaagcgc
    10681 cgcgcccgct ccagggcctc ggccagatgg ggggcgccga gcatgccgcg gcacaggaat
    10741 tcgaagctgc cggtgcccag cgggcgctcg aaaaggccga agccttcgtc gtccagatcg
    10801 cggatcaccc ggttgtacag cgccgcgtag cgatccagcg ggatgcggct ggccgagtct
    10861 gcaaggtcga tgtcggtggc ggccagcagc ggcgccgggt cgtggccccg ccgctgcatc
    10921 ccggcgacca tcccggtgac gaagcccatg gccacgctgg cccgcctgcg ctccggggcg
    10981 gcgggcgatg gggcgggggc ggcggaagag gagctcatgg gcatctccgg gaccggctga
    11041 agggccaatc tgcagcttgg cggattttgc acgaatcccg acgggaacag caatggcgca
    11101 gccgcgtccg gccttctacc atgaacctat tcctaacaac agtggtggag gagagaagat
    11161 gactgacctg agcgtggcca agaagctggt gacctacaag cccaagaaca aggtgcgttt
    11221 cgtgacggcc gcagccctgt tcgacgggca cgatgcgtcc atcaacatca tgcggcggat
    11281 cctgcaatcc accggctgcg aggtgatcca cctggggcac aaccgctccg tgggcgagat
    11341 cgtcaacgcg gccctccagg aagacgtgca gggtatcgcc atcacgtcct accagggcgg
    11401 tcacgtggag ttcttcaagt acatgatcga cctgctgcgt gagcacggcg gcgagaacat
    11461 ccgcgtcttc ggcggcggcg gcggcgtgat cgtgccggcc gagatcaagg agctccacga
    11521 ctacggcgtc acccgcatct actcgccgga agacggcgcc atcctcggcc tgcagggcat
    11581 gatcaacgac gtggtcgagc gcagcgacat ggacctctcc cacgccggcc cccagggcgc
    11641 cgacgcggtg ctggcggccc tgcgcagcgg cgaccggcgt gccctcgccc ggatcatcac
    11701 cggcctcgag aacggcgcct acgcggatga agtgaagacc gcgctgatcg ccgccgcgaa
    11761 ggagctcaag acgcccaccc tgggcatcac cggcaccggc ggcgcgggca agtcctccct
    11821 caccgacgag ctggtgcggc gcttccgcct ggaccagcac gaccgcctca agctggccat
    11881 cgtgtccatc gacccgtcgc gcaagcgtac cggcggcgcg ctgctgggtg accgcatccg
    11941 catgaacgcc atcgagcacc cgaacatctt catgcgctcc ctggccaccc gcgataccgg
    12001 caaggaagtc tccgtggccc tgcccgaagt catcgccgcc accaagctgg cgggcttcga
    12061 tctggtcatc gtcgagacct ccggcatcgg ccagggcgac gcggccatcg tgcccttcgt
    12121 ggatgtgtcc ctgtacgtga tgacccccga gttcggcgcc gccagccagc tcgagaagat
    12181 cgacatgctc gacttcgccg acttcgtggc catcaacaaa ttcgaccgca agggcgccga
    12241 ggatgccctg cgcgacgtgc gcaagcaggt ccagcgcaac cgggagctgt tcaccaccca
    12301 ggtggagcag atgcccgtgt tcggcaccat ggccgcccgc ttcaacgacg acggtgtcac
    12361 cgccctgtac caggccctcg ccccggccct cgtcgccaag ggcctcaagc tcggcaagcc
    12421 cggcctgccc ctggtgagcg tgcgcgcctc gtcccgcggc cgcgccatcg tccccgccga
    12481 gcgtgcccgc tacctggccg agatcgccga cgccgtgcgc ggctaccaca agcacaccct
    12541 ggagcagtcc cgcatcgccc gcgagcgcca ggccctgcgc atctccaagc gcctcttcga
    12601 aggctgccag aaggacgccg gcagctttga cgagctgatc gactggaagg acggcgagct
    12661 gaccccgtcc tcccgcaagc tcctcgacat gtggcccaag accgtcgagc tgtacgcggc
    12721 cgacgagtac gtggtgaaga tccgcgacaa ggagatccgt accgccctca cctccgagtc
    12781 cctgtctggc agcaagatcc gcaaggtggc cctgcccagc ttcgaggacg acggcgagtc
    12841 cctgcgcttc ctgatgaagg agaacgtccc cggctccttc ccctacaccg ccggcgtgtt
    12901 cgccttcaag cgcgaaggtg aagacccgac ccgcatgttc gccggcgagg gcgatgcctt
    12961 ccgcaccaac cggcgcttca agaaggtctc ggaaggcatg cccgcgcacc gcctctccac
    13021 cgccttcgac tcggtgacgc tctacggctg cgacccggac ccgcgtccgg acatctacgg
    13081 caaggtcggt aactccggcg tgtctatcgc cacgctggac gacatgaagg tgctctacga
    13141 cggcttcgac ctggtcaacc cgaccacctc ggtgtccatg accatcaacg gcccggcgcc
    13201 catcatcctg gccatgttct tcaacaccgc actggcccag caactggaga agttcaaggc
    13261 ccagaacggc cgcgaaccca ccgacgacga agcccagaag atccgcgaat gggtcctcgc
    13321 caccgtgcgc ggcaccgtcc aggccgacat cctcaaggaa gaccagggcc agaacacctg
    13381 catcttcagc accgagttcg ccctcaagat gatgggcgac atccaggagt tcttcgtcca
    13441 taacaaggtg cagaacttct actcggtgtc catctccggc taccacatcg ccgaagccgg
    13501 cgcgaacccc atcagccagc tcgccttcac gctgtccaac ggcttcacct acgtggaaag
    13561 ctacctggcc cgcggcatgc acatcgacga cttcgcgccc aacctctcct tcttcttcag
    13621 caacggcatg gacccggaat actccgtcat cggccgtgtc gcccgccgca tctgggcggt
    13681 ggccatgaag aacaagtacg gcgccaacga acgcagccag aagctcaagt accacatcca
    13741 gacctcgggc cgctccctgc acgcccagga gatggacttc aacgacatcc gcaccaccct
    13801 gcaggcgctg atcgccatct acgacaactg caactccctg cacaccaacg cctacgacga
    13861 ggccatcacc acgcccaccg aggaatccgt gcgccgcgcc atggccatcc agctcatcat
    13921 caaccgggag tggggcctcg ccaagaacga gaaccccaac cagggcgcct tcatcatcga
    13981 ggagctcacc gacctcgtcg aggaggccgt gttgaaggag ttcgaggcca tcgcgtcgcg
    14041 tggcggcgtg ctcggtgcca tggaaaccgg ctaccagcgc ggcaagatcc aggaggagtc
    14101 gctctactac gagcacaaga agcacgacgg ctcctacccg atcatcggcg tcaatacctt
    14161 cctcaacccc aagggcatgg cccagcagga aatcgagctg gcccgttcct ccgaggacga
    14221 gaagcagagc cagctccagc gcctcgccgc cttccaggac cgcaattccg acgaagcgcc
    14281 ccagtggctg gccaagctca agcagaccgt gatcgagaac ggcaacgtct tcgccgtgct
    14341 ggtggatgcg gtgaagtact gctccctcgg ccagatcacc accgccctgt acgacgtggg
    14401 cgggcagtat cggcgcagca tgtaagactc gcaccgcatc acaagaaccc tcggtaagag
    14461 gggaacagca gaacggcgcc ttcgggcgcc gttcgtttaa gtgcaccagc accttgtggc
    14521 ggcggcgaga ggaccggtta ctcgtggaga tgcatggctc atgctaggat gaccatgatt
    14581 gatgaccata ggggacaggc catgcacacc accatcggtg cgggtgactt caaggcgcgg
    14641 tgcctgaaat tgctggacga cgtggcccgt tccggcgagg ctctggtgat tacgaaacac
    14701 ggcaagccgg ttgcgaagct ggtgccgatg ccgcccgccg ggagcctgtt cggtgcgctg
    14761 gccggcagtg tgcggcagga gggcgacatc gtggcgcccg tcgatgacga ctgggatgcc
    14821 tgtcgttgag ccccctgctg ctggacaccc acgtcctggt ctggctgctc gccggcagcg
    14881 atcgtctcgg cctccgtgcg cgggccgcca ttgatgcggc agccgcggtc gaaggggtgt
    14941 tcgtgtcggc catcaccccc tgggaaattg ccatgctcgt cgccaaggga cgtctggtcc
    15001 tggagcggga tgtgggcgac tgggtgcagg cggctctggc cttgccgggc atcaggctcg
    15061 aagcgctctc gccggccgtg gcggtggcga gcacgcggct gcccggcgcg ctgcacgccg
    15121 atccgacaga ccggatcatt gtcgccactg cccgccacct gggcgcagcg ctggtgacgg
    15181 ccgacggctt gctgctcgac tacgcctcga gcggaaatct gaaggtgttg ccggcggggc
    15241 agtagcacgt cactctatgc cgccggtcct ccgcgaagac ctgctctgtg cgccgtggga
    15301 cgcgctcagc gccgtccgag cgggatcgtc ccgaggcgcg ggccacggat ccgctcccct
    15361 cactgccccg ccagcgcatc caccatcgcc cgcagcgtgc ggatctgcac gccctcgcgg
    15421 gtcgcgtaat cctgtcccgt gtagccccac cagtcccagc agccgttggg gttgccgggc
    15481 accgtgaggg cctggggata gaggatcacc aggtgattgt tgtcggccca gcggtcgtag
    15541 cccgtgtgct tcacgaacag ggtgccgttg tacaggccgc cgtcgggcgg cgggcgcagg
    15601 ggcatgtagc tctggccctg cttgcagccg tgcagcacga tgtgcacgcg gcagcgctca
    15661 cccttgcggc agctgttggg cacgtagagc cagccggtcg cgtccatgcc cgacttgaag
    15721 cccgtgtaat cgcggcgttc ctccggcacg tagcggcgct ggtcgaactc cgagaagctg
    15781 cccgccgcct tggccccggc cggcgtcagc gcgccgtaga tccattgcag gatcgccccg
    15841 gcgctgtcgt agccgcactt gccgatgtag ggcgcgtcct ccttgccgca ggcgatgccg
    15901 tagctgctgg tcggcatggt gtgggccgcg ttgggcaggg acaccggcga gatctgcgcc
    15961 gccggcacgc cgagggcggc atagaaggcg tccagctgct gcgcgatggc cggcggcacg
    16021 agggtgtcct tggcaccgga caccgtcacg atgcgctgcc gcgccagatg ggacggcgcg
    16081 tcgatggcgc cctgggcggc cagggtcttc accttgtcgg tgagggtcgg cacgtcggcg
    16141 ctgtggtccg tcacctggca ggcgacaaac ggcttgccgg tgcacgagca ctgggacacc
    16201 gcccgcgtcg tgtcgccctg ggcgcaggcg tagggcccgc ccgccaccac cccggcgccc
    16261 gccacgatgg acgaatgggc cacctggaac tgcacgctca tgaagccccc ggacgagatg
    16321 ccggagaccg aggtctgatt cacgtcgatg ttgagggcgg gcagcggcgg cgctgcctgc
    16381 agggcgggcg acaccagcgt cgcgaggacg acaccggcgg cggccagggc gcgggccggc
    16441 agggcacgga aggacatggg caactccagg gtttgtcggg agggccggca agggcgccgg
    16501 gcgaagcacg cgcgtgatgc gccgcaacat caccttagcc caagaagcct catgcgaaaa
    16561 gaattttcgg tgacgtcgcc cggcaggtcg atttcagacc tgtgggggag ggaacggccc
    16621 gcccgtggga aggtcgcagc ccccgcgcgg tccttcccgc gcactccgtg ctgtccggca
    16681 acgggccgcg tctcagggcc gtgccccgcg tgccgcccca tgccggcgcg ccgtcgtcct
    16741 cacatgccgc cgcgcgccgc gcccagcatc tcgtgcacct cgtagctcat caccgccaga
    16801 taggccagca ggcccaggta gagcagcgcg tactcgaagc gcttgcctgc cggcgtgtcg
    16861 taatagcccg gcgcctcgcc cccttcgccg cggaaggccc ggagcatgtg gggcgcggcg
    16921 aggatcgcca cgaggatcag catcgggctc ggccgccaca ggaacaggcc caccagcacc
    16981 ggcgctccca gcagccagaa gcgccgcgac accacgccgg tcatgcgccc gccgtccagg
    17041 ggcggcaccg ggatcaggtt gaagagattc aggaagaagc ccgaatagct gatcgccagc
    17101 agcaggtcac tcccctccga gcgggccagg aagtagcacg ccaccgaagc cagcgtgccc
    17161 gccagcggcc cggccatcgc cacataggcc tcggtctccg cgttcatggg ctgctccttc
    17221 aggtcgatcc acgcccccac gaaggggatg aaggtcggca cgcccacgtg cagcccgcgc
    17281 tggcgcgccg cgatgtaatg gcccatctca tggcagaaca gcagcaccac gaagcccacc
    17341 gcgtagcgcc agccccacac gaaggcatac accgcgaccg acagcagcat ggtgccgccg
    17401 gtggtgagca gcttgccgaa cttcagcccc gagagcagca gcaggagcag cttcacgcct
    17461 gcccgtcctt gcgcccgaag agcttgcgca gcccggcgcc ggcgcccacc accgccagcg
    17521 ccgccacctt ccagaacttg gccaggaaca cccccagcgt cgccagcagg cccagcttct
    17581 tcgccgcgat gccccccacc agcgccgcca gaccgtattc cgccaccttg tcggtggacg
    17641 cgttgaagtc cgcgtagcgc ttgccttcgt caaaggacaa ggccgccaac aactgctggg
    17701 ccaccggctt ctgctgctcc acggtcgcca ggctggtcac caggttcatg gagaagtaac
    17761 cctcccggcc cagcacataa gtgttgtagt tcaccccgtc cccgtcgctc gcggccccgt
    17821 tcttctcgcg ggtagaggcc gaccacacca ggcggtggtt cgcctcgtcg tagcggggcg
    17881 cctccaccca gccctgcacg ctgaactccg gcatgcccag cttgcggcgg tcctcgttgg
    17941 ctgcctcggt gccggacttc aggttgtcca gcagcttcgc cgcgtcccac tcgcgggcct
    18001 cctcgtcctt cacgtagccc gcgtcctcga agcgcaccac cacaaagccg tcgatcgcat
    18061 cgcccaccac cagaccctga aagtccggcc ccacgcggga gccctgcgcc ttcaggaatt
    18121 cccccgccgg cccggcgggg atgaagccga agccctccgg cagcttgagc acgccctgcc
    18181 cggccagcgc aatgtcccgc ggccccttct gcagcgccgc ctgggccgcc tgcgcagccg
    18241 ccgccagccg cgcctgcgga tcggtggccg ccgcgggcga cgaggc
//
LOCUS       DAOQPA010000073        17951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1571_length_17951_cov_16.928755, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000073 DAOQPA010000000
VERSION     DAOQPA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_17951_cov_16.928755"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..2255)
                     /locus_tag="PKX01_10975"
     CDS             complement(126..2255)
                     /locus_tag="PKX01_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76694.1"
                     /translation="MEPNTENLLGIIADSPDFIGVIDLDGHLKYHNKAACRLLGRPDD
                     ADLTALSLPTVHPPWAAQLLRDTAIPTALATGHWEGESAILRPTGREVPVWQEILPQR
                     DVSGAPTLLSIIIRDISAQKLSQNLEKHRSTVLQFLVDAAPLATVLEAIVVGIERIDP
                     TALTSILLLDDTGRHLLKGAAPRLPDFYNKAIETLEIGFGRGSCGTAAATGSRVIVED
                     IATHPYWAPYKELAAHAGLASCWSEPIRDSHQKVLGTFAIYHRHPSAPTEEDFALIGQ
                     ASSLASIAIERRRTEMELERYRNHLEERVEERSRTINDLNRELEQRVRDAENATREKS
                     EFLANMSHEIRSPLNAIIGLTHLMRRSATDADQIDKLGKISTAGHHLLSVINDILDFS
                     KIESGNLELESNIVELRTLAGNITSMLEIAARDKGIALKVDTGDIPRQVRGDSTRLTQ
                     AFLNLANNAVKFTEHGTITLRIRLLEETADTVLLRFEVTDTGIGVAPEILPTVFSSFR
                     QADSSTTRRFGGTGLGLTITRRLAELMGGEAGAESTVDVGSTFWFSARLAKVLESDAA
                     RRAAVPPSEIEENLRQHFAGTRILLVEDDIINQMVGQELLESVGLTVDIAHDGVDAVE
                     RMRGPDAERYALILMDMQMPRLDGPGATRQIRALPALPKRPILAMTANAFGEDRERCL
                     SAGMDDFITKPVNPDILYAKLLQWLST"
     gene            complement(2796..3710)
                     /locus_tag="PKX01_10980"
     CDS             complement(2796..3710)
                     /locus_tag="PKX01_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HMZ76695.1"
                     /translation="MIVRPHQHWFRRLFDWHGSVLPNILFRLSLNVMMGIVAVIALPW
                     YEQLDIHLTTAPFSLLGVAIAIFLGFRNNTSYGRYCEARLFWGSLLVTTRSLLRQWKS
                     ALPDAASAREFAGLLLAFTWSLKHQLRGSSPQDDLRRLLPERYLADVSASPLTAHRIL
                     LHLDLWLGDRRRAGQISDVLYQSFDANLNELSRILGGLERLASTPIPFAYSLILHRTV
                     YLFCTLLPFALVSDLHAMTPVVSVFISYTFLSLESIAEELEDPFGTAPNDLPLNALCT
                     TIEIQLKELNDDHPLPPRPQPDAHFNLT"
     gene            complement(3969..4967)
                     /locus_tag="PKX01_10985"
     CDS             complement(3969..4967)
                     /locus_tag="PKX01_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HMZ76696.1"
                     /translation="MTPPALPRLLRTLHKEWFLVGMIAAVLLASLAPNLGRSGGVLHG
                     DRVADAGIALVFFLHGLGISLTAFRNGLGRWQVHLVVQLCTFVVFPLVGLAAYALLGR
                     WLPAGLALGFCYLCALPSTISSSVAMTGLARGNVPAAIFNATLSSLLGIVATPLLVAA
                     FTGVQGDGLPFMASVIGIARLLLLPLVAGQLMRPLLHAWHTRHKRLTTLLDRWVILLL
                     VYSAFCDSVASGLWQHNGLASLGIAFIGAGAFLAFILAFSTWLVRRCAFPVEDEIATV
                     FCGSKKTLASGVPMAKLLFGAHPSIGLILLPIMLYHQLQLFVCSLLANRYAARSAG"
     gene            5099..5863
                     /locus_tag="PKX01_10990"
     CDS             5099..5863
                     /locus_tag="PKX01_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ76697.1"
                     /translation="MKRILPALAALPRPVFGRNESLAPGMWTDVHTHPWCQLSYAVSG
                     VLAVATPQGDYVAPPHYAVWIPPEVPHQVFNRGRTEMRSLYLENGAPGLPTACAVLEV
                     APLLRELILKVGALPPDYVPDGPAGRLVAVLRDELAAARQAGFALPLAKDRRLLPIQA
                     ALQDNPADARTLAEWATQVGASERTLARCFVKETGLGFGDWRRRLRLLLSLSALEAGD
                     SVTAVALAAGYDSTSAFIAAFREVFGFTPGSLPGRS"
     gene            complement(5873..8338)
                     /locus_tag="PKX01_10995"
     CDS             complement(5873..8338)
                     /locus_tag="PKX01_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ76698.1"
                     /translation="MSTSVITMSPPHDAPATIEEALLSRDALYRNIIASAADGFFTVD
                     ARYRFLDVNQAFCTLFGCPRETFIGRSPLDFVTPASRPELTRQIGRVATTQRRVYELE
                     GLRPDGSTFPALISTVTHRNVRDEIVGTLGFITDLGPAYAARARVAQSEAELRAVLDN
                     LQDTYYRTDADGCLVRVSPSVRKLLGYDAEELIGTPIARLYTRPTERDVFLQTLEDAG
                     GAVEHYEASLRHKDGHEVWVATSAHLYRNPDDDTVGVEGTTRDISRERAQRVQLQLAA
                     TVLAATREAIMITDHDNRIISVNPAFTQLTGYQPEEVIGQGPQLLSAQRHSIAFYSQL
                     WDALAGDDHWSGEVWNRRKNGNYFPQWLSLSAVRDPAGKLLHYVAIFSDLSDRKAAEA
                     RIEFLAHHDALTCLPNRLLLKERAQQCFVQAERNRAKPALLFLDLDRFKDIIDSLGHP
                     IGDRLLQAVAERLRSATRESDLVGRLGGDEFLVLLNGIRDTEDIAPVVEKIMQALAEP
                     YHADGHELLCTCSVGLAVFPDDGTDFDTLAKHADAAMYEAKAAGRATFRFYSQRMNED
                     ALARLDLQKGLRRGLERNEFILHYQPLVDLADNRIIGAEALLRWQPPDRPLVPPGHFI
                     PVAEDSGLIVPIGAWVLREAFTQARAWQDAGHQLVMAINLSPLQITRGDLVDTVRALL
                     ADTGADPHRIELEITESVLLHDTEQVLAVVRQLRQMGLRLSIDDFGSGYSSLAYLRRF
                     AVEKLKIDQCFIRDLPTDPEDGAIVRAVIQMAKSLKMTVLAEGVESADIAHDLRLLGC
                     DLAQGYHFGRPMPAQAFLDLLGA"
     gene            complement(8390..9223)
                     /gene="metF"
                     /locus_tag="PKX01_11000"
     CDS             complement(8390..9223)
                     /gene="metF"
                     /locus_tag="PKX01_11000"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HMZ76699.1"
                     /translation="MTPNLSIEFFPPQTPEGADKLRVVRQKLAVLKPEFFSVTFGAGG
                     TTQDRTFDTVFEIRSEGHEAAPHLSCIGSTREHIREILHRYRDAGIRRIVALRGDLPS
                     GVGFAGELRYANELVEFIRAETGDHFHIEVAAYPEYHPQARSPRDDLLAFQRKVAAGA
                     DSAITQYFYNPDAYLHFRDECDKLGITVPIVPGIMPIVSFSKLARFSDACGAEIPRWM
                     RRKFESFGDDSDSIRAFGLDVVTGLCEKLLAAGAPGLHFYCMNQSGPTLALCERLGIG
                     A"
     gene            complement(9296..10708)
                     /gene="ahcY"
                     /locus_tag="PKX01_11005"
     CDS             complement(9296..10708)
                     /gene="ahcY"
                     /locus_tag="PKX01_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMZ76700.1"
                     /translation="MNAVAETFTDCVVADLSLADWGRKEITIAETEMPGLMAIRDEFA
                     KTRPLQGARITGSLHMTIQTAVLIETLIALGAEVRWASCNIFSTQDHAAAAIAAARIP
                     VFAVKGESLTDYWDYTHRIFEWADGAYSNMILDDGGDATLLLHLGARAEQDLSVLDSP
                     DSEEATVLFAAIKAKLKTDPTWYSTRLARIQGVTEETTTGVHRLYQMHERGELKFPAI
                     NVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQALRALS
                     AQVWITEIDPICALQAAMEGYRVVTMDYAADKADIFVTATGNYHVIDHHHLVAMKHNA
                     IVCNIGHFDSEINVASLEAYPWEEIKPQVDHITLPSGHRIILLAKGRLVNLGCATGHP
                     SYVMSSSFANQTIAQIELFTKTADYPVGVYTLPKHLDEKVARLQLKTLNVALTTLTDA
                     QAQYIGVPVDGPYKSAHYRY"
     regulatory      complement(10801..10910)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(10960..11592)
                     /locus_tag="PKX01_11010"
     CDS             complement(10960..11592)
                     /locus_tag="PKX01_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMZ76701.1"
                     /translation="MPTAPFAAPDTPPARSDLLRDLSMGLLLDELAFGIAVLARDRTL
                     IYASRTALAHFHPGGVLQILDDLVEAPNARDALLLQDALDDVQSGLRTYLSLGHGEHS
                     VDVAILPLTGMVTDSGTPAAIVFEQSTQTAGLSLYFYARAHGLTRAEERILGALYNGA
                     SVDDVATRIGCTPNTARTHVRRVLEKTGQPTLRALTARLGRLPPMAGQAD"
     gene            complement(11746..12588)
                     /locus_tag="PKX01_11015"
     CDS             complement(11746..12588)
                     /locus_tag="PKX01_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76702.1"
                     /translation="MAASPEPPQPEALAVRVWIARRRQNRDALDNITGPALSREAFLT
                     ALRQTFVPATPQLMAPLGLLAYFPTLPPVVDAYPDLPEETALVFYSSRDAYDIARRTP
                     SGRLYAASHGLYFDALAARWPERWQPGDASVPGQAYCGPAHRDGDWNEGQVWTFMLAW
                     QDAPVTDSWMRDLCAPCLAGIAPIRQWVAHYTDGCLVVWFHSPQPATAQALQIRQALR
                     DARLPGAPDTIYLHAARDVTVAALTEPGSGLDFHEDETLHVCYRPPIAPPPASPGPDA
                     SAPP"
     gene            complement(12597..13559)
                     /locus_tag="PKX01_11020"
     CDS             complement(12597..13559)
                     /locus_tag="PKX01_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76703.1"
                     /translation="MSWTASDFERLDPKLFRGFLAVVRSGSVSEAARIASLTQGAVSQ
                     QIARLEERLKCQLFVRANGRILPTDAAQLLAEFAESYLQSSSSFLERLNAEFESIQGL
                     VAYSMPESCIHSPHFPQLLARRLEFPGIHLRVTLKPTDAVSRDVLGGEVDFGFVLEKR
                     HEKQLIHHPFCVEEYGLVAAPSLHPRPLSHLDDIVRLPYVCFPESAFYLHRWLSGLAR
                     REMSPDADDLSVRGEFSDLRGAIAMVKGGMGATVLPLHVVADDLAAGRLVRIRHRDND
                     PPVTQQISICRLAERRLPARVRRVIRWFLEMHADLQPVPADFLN"
     gene            complement(13622..15196)
                     /locus_tag="PKX01_11025"
     CDS             complement(13622..15196)
                     /locus_tag="PKX01_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76704.1"
                     /translation="MKPPIEVALMDEVRSCRDCTSLWRAPDRFGPFPLCDGAALDALP
                     AARADAPAPTPQAVTVEGFGLPDPAALRGCRKAPVMIVGINPNLTGFWTLPDAPRYAL
                     KGPSTVYARHRSLAEYARAHRYVTPDHHEYRMRPEDVETLIDATRHDLRAAGAGSVIP
                     VDKKHPAASPTGRQSHARHVTLGLQFDDGGTDTTQFDWLPDEHYVTLRARFAAGERIG
                     GVMTAASVQGRTIAVRADVGSSYYARAQEICRRSDLVPGEDISLHDMVACATQGWSDK
                     TGVDRDELSAACIDSRRHALRQIRQSAPRLLIFSGLEALGLFLRAVGLPAFPALGPDV
                     TVTRLLAGLKTELDTGALTRRLALHLPADGDLPPWRAHVVAVPHLSYPIRPIAFDAGV
                     ASPLWSRFAEHCPASFALIAQAPENSGTGAHALLDTATPSFRLLSDTWQTIDQLDEGD
                     ALYRLVEIGGDTRIEALASLIAQLRHSGDLPERAPDRAANARLLRSAGPCHFCPAFAV
                     PEGCIFEFADFANTEL"
     gene            complement(15193..16536)
                     /locus_tag="PKX01_11030"
     CDS             complement(15193..16536)
                     /locus_tag="PKX01_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76705.1"
                     /translation="MPTPSLPCLAIGGDTRHGAGRPGTGTDPWWSALALGASGLATIL
                     HSVDGRLECAGPTGLDGVPSLSLDDFLWTFGQACPLAFLVPHETPLTHDQVQACARRL
                     LGAALRHPPRLIVPATMLADVIATGFDTACSLNPYAEPSADDHQAWSSDSAAHRFPDL
                     PFPGGRSDGAYLLVRRGRSGQFQPDVWLTTDADLDARAQQSAILLDDDFTDGAASRRW
                     IFGYSNPVPESFILFGPPEAPAFIVDMPAGTGYSGGAAVLARALVTDFDARITFVSED
                     PAQGTTLELAALTIDPPRRSLVDRSHTADDKYRSLCFDVHGAPPYVSSECDENDGFRI
                     GWNLAGALARIDDSVTASSNHYNRYGEDVGGPLKGPISGELRLVRCGEFWASYFRLGG
                     ESAWRCSGALHIPDLPRRIHLRAGAKHWVKDGRPAPRNRVSFTTATVLIPAWSQP"
     gene            complement(16767..>17951)
                     /gene="dsbD"
                     /locus_tag="PKX01_11035"
     CDS             complement(16767..>17951)
                     /gene="dsbD"
                     /locus_tag="PKX01_11035"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HMZ76706.1"
                     /translation="FPMIPILSGIIVGHGHAISRGRAFTLSLLYVLGMALTYTAAGVA
                     AGLSGTLLSAALQNPWVLGGFALVFVALSLSMFGFYELQLPTALQSRLSDRANHQPGG
                     SPAGVILMGALSALIVGPCVAAPLAGALLYIARSGDALLGGAALFTMALGMGAPLLLV
                     GVAARSALPRPGPWMEGVKRAFGVMLLGVAVWLVSPVLPAVVPMLAWAGLLIMSAIYL
                     HALDPLPPHAAGWQRFWKGIGVVALLTGAALFIGALAGSRDPLQPLAILRAQAAAPAE
                     GPHFRRIASGAELDEILKTADRPVMLDFYADWCVSCKEMERFTFADPAVARRLAGVLL
                     LQADVTANSEDDKALLKRFDLFGPPGILFFDAHGAERRAQRVIGFQAADAFLRTLDAA
                     LP"
BASE COUNT         2753 a         4996 c         7393 g         2809 t
ORIGIN      
        1 cccaggcggt gcagggaagg cggtgtgcgg tgcgaagtgc gccgccggga gggcaccagg
       61 gcgcagccgc ccgcgtcggg atggggcgcg tcaggcgggt gtcaccttcc cgccggccgt
      121 gtctttcagg tggacagcca ctgcagcagc ttggcgtaga ggatgtccgg attgacgggc
      181 ttggtgatga agtcgtccat gccggcgctg aggcagcgtt cccgatcttc gccgaaggcg
      241 ttggcggtca tggcgaggat ggggcgtttc ggcagggcgg gcagggcgcg aatctgccgg
      301 gtggcgcccg gtccgtcgag gcgcggcatc tgcatgtcca tcaggatcag tgcgtagcgt
      361 tcggcatcgg ggccgcgcat gcgctcgacg gcgtccacgc cgtcgtgggc gatgtccacg
      421 gtcaggccga cgctttcgag gagttcctgg ccgaccatct gattgatgat gtcgtcctca
      481 acgaggagga tgcgggtgcc ggcaaagtgc tgacgcaggt tctcttcgat ctcgctgggc
      541 ggcaccgcgg cgcggcgggc ggcgtcggat tccagcacct tggcgaggcg tgccgaaaac
      601 cagaaggtgc tgcccacatc gacggtgctc tcggcgccgg cctcgccgcc catcagctcg
      661 gccaggcggc gggtgatggt caggcccagg ccggtgccgc caaagcgccg cgttgtcgag
      721 gaatcggcct ggcggaagga gctgaacacc gtgggcagga tctccggcgc gacgccgatg
      781 ccggtatcgg tgacttcaaa gcgcaggagg acggtgtcgg ccgtctcttc gaggaggcgg
      841 atgcgcaggg tgatggtgcc gtgctcggtg aatttgacgg cgttgttggc gagattgaga
      901 aaggcctggg tcaggcgggt gctgtcgccc cgcacctggc gggggatgtc gccggtgtcc
      961 accttgagcg cgatgccctt gtcgcgggcg gcaatctcca gcatggaggt gatgttgccg
     1021 gccagggtgc gcagttcgac gatgttggat tccagctcca gattgcccga ttcgatcttg
     1081 gagaaatcca ggatgtcgtt gatcaccgaa agcaggtgat gaccggcggt ggagatcttg
     1141 cccagcttgt cgatctggtc ggcgtccgtg gcggagcggc gcatcaggtg ggtcagcccg
     1201 atgatggcgt tgaggggcga gcggatttcg tggctcatgt tggccaggaa ctcgctcttc
     1261 tcgcgggtgg cgttctctgc gtcgcggacc cgctgttcca gctcccggtt caggtcgttg
     1321 atggttcggg aacgctcttc gacgcgctct tccaggtgat tgcgatagcg ctccagttcc
     1381 atttcggtcc gtcggcgctc gatggcgatg gaggccaggg aggaggcctg cccgatgagg
     1441 gcgaaatctt cttcggtcgg cgccgaggga tggcggtgat agatggcgaa ggtgcccagc
     1501 actttctggt gggaatcgcg gatgggctcg gaccagcagg acgcgaggcc ggcgtgggcc
     1561 gcgagttcct tgtagggcgc ccagtagggg tgggtggcga tgtcctccac gatgacccgg
     1621 ctcccggtgg ccgcggcggt gccgcaggag ccacggccga agccgatctc gagggtttcg
     1681 atggccttgt tgtagaagtc cggcaggcgc ggggccgcgc ccttgagcag atgccggccg
     1741 gtatcgtcga ggagcaggat gctggtcagg gcggtggggt cgatgcgctc gatgcccacg
     1801 acaatcgctt cgagcaccgt ggcgaggggc gccgcatcca cgaggaactg caggacggtg
     1861 ctgcggtgct tttcgagatt ctgggaaagc ttctgggccg agatgtcgcg gatgatgatg
     1921 gagagcagag tcggcgcgcc ggacacgtcc cgctggggca ggatctcctg ccacacgggc
     1981 acttcgcgcc cggtgggccg caggatggcg ctttcgcctt cccagtgccc ggtcgccagc
     2041 gcggtgggaa tggcggtgtc ccgcagcagc tgcgcggccc agggcggatg caccgtgggg
     2101 aggctcagcg ccgtcagatc ggcatcgtcc ggccggccca gcaggcggca ggcggccttg
     2161 ttgtggtact tcaggtggcc gtcgagatcg atgacgccga tgaagtcggg cgaatcggcg
     2221 atgatcccca gcaggttttc ggtattcggc tccaccgcga cttctccgga aattggctga
     2281 tgcctggtga tgctaaagcc tgcctgcctg tttcggatct gttggaatca acagggcgca
     2341 acatcatccc gggcagtgca agcctattgc tgcgctatag cggtccgtgc gtcaaaagat
     2401 caagggcgtg tcgggcaggg gcgggactag gtcattgttt gatatgagtg tagcccaccg
     2461 cgcgcccgac ccgcgcatcc aatccgcgtg ccgcgacggg cgggcgaggc cggacgcagg
     2521 gcggcgttcc cggtgcgcgc ggatgaattc tgcgcggggc gacgcggggt cggttcatgc
     2581 tttcgggcgg gcttcatgaa cgatatcggc cgtggcatcg acagctgaat gcgcatcgtc
     2641 aacgcgggta tagggcggta tcgcgggccg gcgcggtgca gggcaatggc cgggtcttcg
     2701 ggtgcgcggc ggtgccatcc tatgggggca tgacgaccgg aggcgatggg ggacgccaga
     2761 ccgcgggcgg ggcggtccgg cgtgatggcg gggcctcagg tgaggttgaa gtgggcgtcc
     2821 ggctggggtc tgggcggcag cgggtgatcg tcgttgagtt ccttgagctg gatctcgatg
     2881 gtggtgcaca gggcgttgag gggcaggtcg ttgggggcgg tgccgaaggg gtcttccagt
     2941 tcttcggcaa tcgattcgag ggacaggaag gtgtaggaga tgaagaccga cacgacgggc
     3001 gtcatggcgt gcaggtcgga caccagggcg aagggcagca gggtgcagaa gaggtacacc
     3061 gtgcggtgga ggatgaggct gtaggcaaag ggaatgggtg tgctggcgag gcgttcgagg
     3121 ccgccgagga tgcgcgagag ttcgttgagg ttggcgtcga agctctggta gaggacgtcg
     3181 ctgatctgcc cggcgcggcg cctgtcgccc agccacaggt ccaggtgcag gaggatgcgg
     3241 tgggcggtga gggggctggc gctcacgtcg gccaggtagc gctcggggag caggcggcgc
     3301 aggtcgtcct gggggctgct gccgcgcagc tggtgcttga ggctccaggt gaaggcgagc
     3361 agcagaccgg cgaattcgcg ggcgctggcg gcgtcgggca gggcgctctt ccactggcgc
     3421 agcagagaac gggtggtgac cagcaggctg ccccagaaga ggcgggcttc acagtagcgg
     3481 ccgtagctgg tgttgttgcg gaagcccagg aagatggcga tggccacgcc gaggaggctg
     3541 aagggggcgg tggtgaggtg gatgtcgagc tgttcgtacc agggcagggc gatgaccgcg
     3601 acgatgccca tcatgacgtt gaggctgagg cggaacagga tgttggggag cacggagccg
     3661 tgccagtcga agagtcggcg gaaccagtgc tggtgggggc ggacgatcat ggggtgggcg
     3721 aacaggacag ggctccggcg ggatgcggcg ttctgcgacg cctgccgcga cggggcgggg
     3781 tggatcgggc gagtggggcg gcctgcccgg cgaggggcgc ggcgtcgggg cgtattgtgg
     3841 cctgaagtgg ccgtcttgag ggagaggcgg cccctgtggt cgggccgcag gatgagcggg
     3901 ggcccccagc gcaggtcgtg gccggtcccg cgcagaccgc ggggccggga gatgccgggg
     3961 cggtgcgttc agccggcgct gcgcgctgcg tagcggttgg cgagcaggga gcagacgaag
     4021 agctggagct ggtggtacag catgatgggc agcaggatca gcccgatgga aggatgggcg
     4081 ccgaagagca gcttggccat gggcacgccg gaggcgaggg tcttcttgga gccgcagaag
     4141 acggtggcga tctcgtcttc gaccgggaag gcgcagcgcc gcaccagcca ggtggagaag
     4201 gcgaggatga aggcgaggaa ggcgccggcg ccgatgaagg cgatgcccag ggatgcgagc
     4261 ccgttgtgct gccacaggcc ggaggcgacc gagtcgcaga aggcgctgta caccaacagg
     4321 aggatcaccc agcggtccag cagggtggtg aggcgcttgt ggcgggtgtg ccaggcgtgc
     4381 agcaggggac gcatgagctg gccggccacc aggggcagca ggagcaggcg ggcgatgccg
     4441 atcaccgacg ccatgaaggg caggccgtcg ccctgcacgc cggtgaaggc ggccaccagc
     4501 aggggcgtgg cgacgatgcc cagcaggctg gagagggtcg cattgaagat ggcggcgggg
     4561 acgttgccgc gggcgaggcc ggtcatggcc accgacgagg agatggtgga gggcagggcg
     4621 cacaggtagc agaagccgag ggcgaggccg gcgggcagcc agcggccaag cagggcgtag
     4681 gcggcgaggc ccacgagggg gaagacgacg aaggtgcaca gctgcacgac gaggtgcacc
     4741 tgccagcggc ccaggccgtt gcggaaggcg gtcagggaaa tgccgaggcc gtggaggaag
     4801 aagacgaggg cgatgccggc gtcggcgacg cggtcgccat ggagaacgcc gccgctgcgg
     4861 cccaggttgg gggcgaggct ggcgagcagc acggcggcga tcatgccgac gaggaaccac
     4921 tccttgtgga gggtgcgcag gagtctgggc agggcggggg gcgtcatggg cgtctcctct
     4981 ggcttgcgat gcggggggat gaggtggaac gatacgggcc ttcggtctgg ccggctaacg
     5041 gcatcaggcc attccatgcc gcgaaactga catctccagg atgtcgagag gtgaatcggt
     5101 gaaacgtatc ctgcccgcgc tggcggcttt gccccgtccg gtgttcggcc gcaatgaatc
     5161 cctggcgccg gggatgtgga cggacgtcca tacccatccc tggtgccagc tttcatacgc
     5221 ggtgagcggc gtgctggcgg tggcgacgcc tcagggggat tacgtggcgc cgccccacta
     5281 cgcggtgtgg attccgccgg aggtgccgca ccaggtgttc aaccggggcc gcacggagat
     5341 gcgcagtctg tacctggaga acggcgcgcc ggggctgccg acggcctgtg cggtgctgga
     5401 ggtggcgccg ctgctgcgtg agctgatcct gaaggtgggc gcgctgcccc cggactacgt
     5461 gccggacggc cccgccgggc ggctggtggc ggtgttgcgg gatgaactcg ccgccgcgcg
     5521 gcaggcgggc tttgcgctgc ccctggcgaa ggaccggcgt ctgctgccga tccaggcggc
     5581 gctgcaggac aacccggcgg atgcccgcac gctggccgaa tgggccacgc aggtgggggc
     5641 gtcggagcgc accctggcgc ggtgcttcgt caaggagacg gggctgggct ttggcgactg
     5701 gcggcggcgc ctgcgcctgc tgctgtccct gtcggcgctg gaggcggggg acagcgtgac
     5761 ggcggtggcg ctggccgcag gctatgactc cacgtcggcc ttcattgcgg ccttccgcga
     5821 ggtcttcggc tttacgccgg gcagtctgcc ggggcggagc tgaacgcggc gttcaggcgc
     5881 cgaggaggtc caggaaggcc tgggccggca tcgggcggcc gaagtggtag ccctgggcca
     5941 ggtcgcagcc gagcaggcgc aggtcgtggg cgatgtcggc gctctcgacc ccttcggcga
     6001 ggacggtcat cttgaggctc ttggccatct ggatgacggc gcggacgatg gcgccgtctt
     6061 ccgggtcggt gggcaggtcg cggatgaagc actggtcgat cttgagcttc tccacggcga
     6121 agcggcgcag gtaggcgagg ctggagtaac cggagccgaa gtcgtcgatg gacaggcgca
     6181 ggcccatctg acgcagctgg cggacgacgg cgaggacctg ctcggtgtcg tggagcagca
     6241 ccgattcggt gatctcgagt tcgatgcggt gggggtcggc gccggtgtcg gcgaggagcg
     6301 cgcgaacggt gtcgaccaga tcgccgcggg tgatctgcaa gggcgagagg ttgatggcca
     6361 tgaccagctg atggccggcg tcctgccagg cgcgggcctg ggtgaaggct tcgcgcagga
     6421 cccaggcgcc aatggggacg atgaggccgg aatcctcggc gacggggatg aagtggccgg
     6481 gcggcaccag ggggcggtcg ggcggctgcc agcgcagcag ggcttcggcg ccgatgatgc
     6541 ggttgtcggc aagatccacc agcggctggt agtggaggat gaactcgttg cgctccaggc
     6601 cgcggcgcag gcctttttgc aggtcgaggc gcgcgagggc gtcctcgttc atgcgctggg
     6661 agtagaagcg gaaggtggcg cgcccggcgg ccttggcttc gtacatggcg gcgtcggcgt
     6721 gcttggcgag ggtgtcgaag tcggtgccgt cgtcggggaa gacggcgagg ccgacggagc
     6781 aggtgcacag cagctcgtgg ccgtcggcgt ggtagggctc ggcgagggcc tgcatgatct
     6841 tctcgacgac gggggcgatg tcttcggtgt cgcggatgcc gttgaggagc acgagaaatt
     6901 cgtcgccgcc gaggcgcccg accaggtcgg attcgcgggt ggcgctgcgc aggcgttcgg
     6961 cgacggcctg cagcagacgg tccccgatcg ggtggccaag ggagtcgatg atgtccttga
     7021 agcggtccag gtcgaggaac agcagcgcgg gtttggcgcg gttgcgttcg gcctggacga
     7081 agcactgctg ggcgcgctcc ttcaggagga ggcggttggg caggcaggtg agggcgtcgt
     7141 ggtgggcgag gaattcgatg cgcgcttcgg cggccttgcg gtcggacagg tcggagaaga
     7201 tggcgacgta gtggaggagc ttgccggccg ggtcgcgcac cgcggagagg gacagccact
     7261 ggggaaagta gttgccgttc ttgcggcggt tccagacttc gccgctccag tggtcgtcgc
     7321 cggcgagggc gtcccacagc tggctgtaga aggcgatgga gtggcgctgg gcggagagga
     7381 gctgagggcc ctggccgatg acttcttcgg gctggtagcc ggtgagctgg gtgaaggccg
     7441 ggttgaccga gatgatccgg ttgtcgtggt ccgtgatcat gatggcctcg cgggtggcgg
     7501 cgaggacggt ggcggccagc tgaagctgga cgcgctgggc gcgttcgcgg ctgatgtcgc
     7561 gggtggtgcc ctcgacgccg acggtgtcgt cgtccgggtt gcggtagagg tgggcactgg
     7621 tggcgaccca gacctcgtgg ccgtccttgt ggcgcaggct ggcttcgtaa tgttcgacgg
     7681 cgccgccggc gtcttcgagg gtctgcagga agacgtcgcg ctcggtgggg cgggtgtaga
     7741 ggcgggcgat gggggtgccg atgagctctt ccgcgtcgta gccgaggagc ttgcggacgg
     7801 aaggggagac gcgtacgagg cagccgtcgg cgtcggtgcg gtagtaggtg tcctgcaggt
     7861 tgtcgaggac ggcgcgcagt tcggcttcgg actgggcgac gcgggcgcgg gcggcgtagg
     7921 cggggccgag gtcggtgatg aagccgaggg tgccgacgat ctcgtcgcgg acgttgcgat
     7981 gggtgacggt gctgatcagg gccgggaagg tgctgccgtc ggggcgcagg ccttccagtt
     8041 catacacgcg tcgctgggtg gtggcgacgc gaccgatctg gcgggtgagc tcggggcggc
     8101 tggcgggggt gacgaagtca agcgggctgc gtccgatgaa ggtctcccgc gggcagccga
     8161 agagggtgca gaaggcctga ttgacgtcga ggaagcgata gcgggcatcg accgtgaaga
     8221 agccgtcggc ggcgctggcg atgatgttcc ggtacagcgc gtcgcgggag agaagggctt
     8281 cctcgatggt ggccggtgcg tcgtggggcg gcgacatggt gatgacggac gtggacaacg
     8341 tccgtcatta cggccgcgcc cggccggact tgaggtccgg ccggacgtgt caggcgccga
     8401 tgccgaggcg ttcgcagagg gccagggtgg ggccggactg gttcatgcag tagaagtgca
     8461 ggccgggagc gccggcggcg aggagctttt cgcacaggcc ggtgacgaca tcgaggccga
     8521 aggcgcggat ggagtcgctg tcgtcaccga aggattcgaa cttgcggcgc atccagcgcg
     8581 ggatctcggc gccgcaggcg tcggagaagc gggccagctt ggagaaggag acgatgggca
     8641 tgatgcccgg cacgatgggg acggtgatgc ccagtttgtc gcattcgtcg cggaagtgca
     8701 ggtaggcgtc cgggttgtag aaatactggg tgatggcgga gtcggcgccg gcggcgacct
     8761 tgcgctggaa ggcgaggagg tcgtcgcggg ggctgcgggc ctgggggtgg tattccgggt
     8821 aggcggcgac ttcgatgtgg aagtggtcgc cggtttcggc gcggatgaat tcgaccagct
     8881 cgttggcgta gcgcagctcg ccggcaaagc cgacgccgga gggcaggtcg ccgcgcaggg
     8941 cgacgatgcg acggatgccg gcgtcgcggt agcggtgcag gatctcgcgg atgtgctcac
     9001 gcgtggagcc aatgcaggac agatgcggcg cggcttcgtg gccttcgctg cggatctcga
     9061 agacggtgtc gaaggtgcgg tcctgggtgg tgccgccggc gccgaaggtg acggagaaga
     9121 actcgggctt gagcaccgcc agcttctgcc gcacgacgcg cagcttgtcg gcgccttcgg
     9181 gggtctgggg agggaagaat tcgatggaga ggttgggggt catggcggat ttccggagca
     9241 agacgggcga ccgtccgggc gccggcagcg ggcgcgcgga cggtgacggg gccggtcagt
     9301 agcggtagtg ggcggacttg taggggccgt ccaccggcac accgatgtac tgggcctggg
     9361 cgtcggtgag cgtggtcagc gcgacgttga gggtcttgag ctgcagacgg gcgacctttt
     9421 cgtcgagatg cttgggcagg gtgtagacgc ccaccgggta gtcggcggtc ttggtgaaga
     9481 gctcgatctg ggcgatggtc tggttggcga acgaggaact catcacgtag ctggggtggc
     9541 cggtggcgca gcccaggttc accaggcggc ccttggccag caggatgatg cggtggccgg
     9601 agggcagggt gatgtggtcg acctggggct tgatctcttc ccaggggtag gcttcgaggg
     9661 aggcgacgtt gatctcggaa tcgaagtggc cgatgttgca gacaatggcg ttgtgcttca
     9721 tcgcgaccag gtggtggtgg tcgatgacgt ggtagttgcc ggtggcggtg acgaagatgt
     9781 cggccttgtc ggcggcgtag tccatggtga cgacgcggta gccttccatg gcggcctgca
     9841 gggcgcagat ggggtcgatc tcggtgatcc agacctgggc agagagggcg cgcagggcct
     9901 gggcggaacc cttgcccacg tcgccatagc cacagacgac ggcgatcttg ccggcaacca
     9961 tgacgtcggt ggcgcgcttg atgccgtcaa cgagggattc gcggcagccg tagaggttgt
    10021 cgaacttgga cttggtgacc gaatcattga cgttgatggc ggggaacttg agttcgccgc
    10081 gctcgtgcat ctggtacagg cgatgcacgc cggtggtggt ctcttcggtg acgccctgga
    10141 tccgggcgag gcgggtggag taccaggtgg ggtcggtctt cagcttggcc ttgatggcgg
    10201 cgaagaggac ggtggcttct tcgctgtcgg gcgagtcgag gaccgacaga tcctgctcgg
    10261 cgcgggcgcc cagatgcagc agcagcgtcg cgtcgccgcc gtcgtcgagg atcatgttgg
    10321 agtaagcgcc gtcggcccat tcgaagatgc ggtgggtgta gtcccagtaa tcggtgaggg
    10381 attcgccctt gacggcgaaa acggggatgc gtgcggcggc gatggcggcg gcggcgtggt
    10441 cctgggtgga gaagatgttg caggaggccc agcgcacttc ggcgccgagg gcgatgaggg
    10501 tttcgatgag cacggcggtc tggatggtca tgtgcagact gccggtgatg cgggcgccct
    10561 ggaggggccg ggtcttggcg aattcgtcgc ggatggccat caggccgggc atttcggttt
    10621 cggcgatcgt gatctccttg cgaccccagt cggcaaggga cagatcggcg acgacgcaat
    10681 cggtgaaggt ttcagccaca gcgttcatga gaactcctcg aacatgtggc cgggaagggg
    10741 atggtacggg tgggctcacc acgtctcccc gtgcccggca cgggtttgca cacaagcatg
    10801 gacgggtgag cgcggttgca ggtacgtcgc ggcgggcgcc gcgggcactg acgtcgagcc
    10861 tgggacggcg gcggcgtggg ccgggtcgtc ttgcagcgct cctcgaaaca ggtcgcggat
    10921 tatatcgggt tttgcggcgg tggcttgagc tgcgtgcgct cagtcggcct gtccggccat
    10981 gggcggcaga cgtccgaggc gcgcggtgag ggcccgcagg gtgggctggc cggtcttttc
    11041 gaggacgcgc cgcacgtggg tgcgggcggt gttgggggtg cagccgatgc gcgtggccac
    11101 gtcgtcgacg ctggcgccgt tgtacagggc gccgaggatg cgctcttcgg cccgggtgag
    11161 gccatgggcg cgggcgtaga agtacaggga caggccggcc gtctgggtgg attgctcgaa
    11221 gacgatggcg gccggtgtgc cgctgtcggt caccatgccg gtgaggggca ggatggccac
    11281 gtcgacgctg tgttcgccgt gcccgaggga gaggtaggtg cgcaggccgc tctgcacgtc
    11341 gtcgagggcg tcctgcagca ggagggcgtc acgggcgttg ggcgcttcga ccaggtcgtc
    11401 caggatctgg agcacgccgc ctgggtggaa gtgggcgagc gcggtacggc tggcgtagat
    11461 gagggtgcga tcccgcgcga ggacggcgat gccgaaggcc agctcgtcga gcagaagtcc
    11521 catggacaga tcccggagca gatcgctgcg ggccggcgga gtgtcggggg cggcaaacgg
    11581 tgcggtgggc atggcaatcc tcaagagcgg aggggccggc gggcgcgggt ggggccggcg
    11641 gtcattcctg catatgctag tggcgcatgc ctgcactgca aaacgatgtc acctaatggg
    11701 ggtcaatagg cttcatgaac ggtctgggcg tcggcgtggg gcgagtcacg gcggcgcaga
    11761 tgcgtcgggg ccaggcgatg ccggtggagg ggcaatgggc gggcggtagc acacgtgcag
    11821 ggtctcgtcc tcgtggaaat cgaggcctga accaggctct gtgagggcgg cgacggtgac
    11881 gtcgcgggcg gcgtggaggt agatcgtgtc cggcgcgccc ggcaaccggg cgtcgcgcag
    11941 cgcctggcgg atctgtaggg cctgcgcggt ggcgggctgg gggctgtgaa accacacgac
    12001 cagacagccg tccgtgtagt gggcaaccca ctggcggatc ggggcgatgc cggccagaca
    12061 gggggcgcac agatcgcgca tccagctgtc ggtgacgggg gcgtcctgcc acgcgagcat
    12121 gaaggtccag acctggcctt cgttccagtc tccatcccgg tgcgccggtc cgcagtaggc
    12181 ctgcccgggg acgctggcat cgcccggttg ccagcgctcc ggccagcgcg ccgcgagggc
    12241 gtcgaagtag aggccgtggg aggcggcgta gaggcggccg ctgggggtcc ggcgggcaat
    12301 gtcgtatgcg tcgcgggagg agtagaagac cagcgcggtc tcttcgggaa ggtccgggta
    12361 ggcatccacc accggtggca gcgtgggaaa gtaggcgagc agtccaaggg gcgccatgag
    12421 ctgcggtgtc gccggtacga aggtctggcg cagggcggtg agaaaggctt cgcggctcag
    12481 ggcggggccg gtgatgttgt caagggcgtc gcggttctga cgtcggcggg cgatccacac
    12541 ccgcaccgcc agcgcttccg gttggggcgg ctcgggtgat gcggccatgg ggatgctcag
    12601 ttcaggaagt cggcaggtac cggctggagg tcggcgtgca tctcgaggaa ccagcggatg
    12661 acacggcgca cgcgggcggg caggcgtcgt tcggccaggc ggcagatgga gatctgctgg
    12721 gtgaccggcg ggtcgttgtc ccgatggcgg atgcgcacga ggcggccagc ggcgagatcg
    12781 tcggccacca cgtgcagggg caggaccgtg gctcccatgc cgcccttgac catcgcgatg
    12841 gcgccgcgca ggtcgctgaa ttcgccgcgg acggacagat cgtcggcgtc cgggctcatt
    12901 tcccggcggg ccagaccaga cagccagcga tgcaggtaga aggcgctctc ggggaagcac
    12961 acgtagggca ggcggacgat gtcgtccagg tgggagaggg ggcgggggtg gagtgaaggg
    13021 gccgcgacca gaccgtattc ctcgacgcag aaggggtggt ggatgagctg cttttcgtgg
    13081 cgcttttcga ggacgaagcc gaagtccacc tcgccgccga gcacgtcgcg gctgaccgcg
    13141 tcggtgggtt tgagggtgac gcgcaggtgg ataccgggaa attcgaggcg gcgcgcgagc
    13201 agctggggaa aatggggcga atggatgcag ctctccggca tcgaataggc gacgaggccc
    13261 tggatcgatt cgaactcggc gttgaggcgc tccaggaagg aactggagga ctgcaggtag
    13321 ctttcggcaa attcggccag cagctgggcg gcatcggtgg ggaggatgcg gccgttggcg
    13381 cggacgaaga gctgacattt gaggcgctct tcgaggcggg cgatctgctg gctcaccgcg
    13441 ccctgggtga gggatgcaat gcgcgcggct tcactgacgc tgccgctgcg gacgacggcc
    13501 aggaatcccc ggaagagttt ggggtcgagt cgttcaaaat cgcttgccgt ccaggacatt
    13561 ttggctccgt ctgggcgatt gttcaggaaa tatcgttatt caggcatata cggagaaggt
    13621 atcacaattc ggtattggca aaatcggcaa attcgaaaat gcagccctca gggaccgcga
    13681 atgccgggca aaagtggcag ggaccggcgc tgcgcaggag gcgcgcgttc gcggcgcggt
    13741 cgggggcgcg ttcgggcaga tcgccgctgt ggcgcagctg ggcgatgagg cttgcaagcg
    13801 cttcgatgcg cgtgtcgcca ccgatctcca cgaggcggta gagggcgtcg ccttcatcga
    13861 gctggtcgat ggtctgccac gtgtcggaga gcaggcggaa gcttggcgtg gcggtgtcga
    13921 ggagggcgtg cgcgcccgta ccggagtttt ccggcgcctg ggcgatcagc gcgaagcttg
    13981 ccgggcagtg ctcggcaaag cgcgaccaca ggggcgaggc gacccctgcg tcgaaggcga
    14041 tcgggcggat cgggtaggac agatggggaa cggcgaccac atgggcgcgc caggggggca
    14101 ggtcgccgtc ggccgggaga tggagggcga ggcggcgggt gagcgcgccg gtgtcgagtt
    14161 cggtcttcag cccggccagc aggcgggtga cggtgacgtc cggccccagt gcggggaacg
    14221 ccggcaggcc gacggcgcgc aagaacaggc cgagggcttc caggcccgag aagatcagca
    14281 ggcggggggc cgactggcgg atctggcgca gggcgtggcg gcggctgtcg atgcacgcgg
    14341 cgctcagttc gtcccggtcg acgccggtct tgtcgctcca gccctgggtg gcgcaggcca
    14401 ccatgtcgtg cagggaaatg tcctcgcccg gcaccagatc gctgcgccgg cagatctcct
    14461 gggcccgcgc gtagtagctg ctgcccacgt cggcgcgcac cgcgatcgtc cggccttgca
    14521 ccgaggcggc ggtcatcacg ccgccgatgc gttcgccggc ggcgaagcgc gcgcgcaggg
    14581 tcacgtagtg ttcatcgggt agccagtcga actgcgtggt gtcggtgccg ccgtcgtcga
    14641 actgcaggcc gagggtgacg tggcgggcgt ggctctggcg accggtgggg ctggcagccg
    14701 ggtgtttctt gtccaccgga atcacgctgc cggccccggc ggcccgcaag tcgtgacggg
    14761 tggcgtcgat gagcgtctcc acgtcctccg ggcgcatgcg gtattcgtgg tgatccgggg
    14821 tcacatagcg gtgggcgcgg gcgtattcgg cgaggctgcg gtgccgcgca tagacggtgc
    14881 tggggccctt gagggcgtat cgtggggcgt cgggcagggt ccagaagccg gtgaggttgg
    14941 gattgatgcc gacgatcatc accggggcct tgcggcatcc gcgcagggcc gcgggatcgg
    15001 gcaggccgaa gccttccacg gtgacggcct gcggggtggg ggcgggcgca tcggcgcggg
    15061 ctgcggggag ggcgtccagg gccgcgccgt cacacagggg aaaggggccg aaacggtctg
    15121 gggcgcgcca cagggatgtg cagtcgcggc agctgcggac ctcatccatc agggcgactt
    15181 cgataggcgg cttcatggct ggctccaggc ggggatgagc acggtggcgg tggtgaagct
    15241 gacccggttg cggggggcgg gccgtccgtc cttgacccag tgtttggcgc ccgcccgcag
    15301 gtggatgcgg cgcggcaggt cggggatgtg gagggccccc gaacagcgcc acgccgactc
    15361 gccgcccagg cggaagtagc tggcccagaa ttcaccgcag cgcaccaggc ggagttcgcc
    15421 ggaaatgggg cccttgagcg ggccgccgac gtcctctcca taacggttgt agtgattgga
    15481 ggacgccgtg acgctgtcgt cgatgcgggc cagcgcgccg gccaggttcc agccgatgcg
    15541 gaagccgtcg ttctcgtcgc attcgctgct cacgtaaggc ggggcgccat gcacgtcgaa
    15601 gcacaggctg cggtatttgt cgtcggccgt gtggctgcgg tccacgaggg agcgccgcgg
    15661 cggatcgatg gtgagggcgg ccagttccag cgtggtgccc tgggccgggt cctcgctgac
    15721 gaaggtgatg cgggcgtcga agtcggtgac cagggcgcgt gcgaggacgg cggcgccacc
    15781 ggaatagccg gtgccggccg gcatgtccac gatgaaggcg ggcgcctcgg gcggtccgaa
    15841 caggatgaag gactccggca cggggttgga gtagccgaag atccagcggc ggctggcggc
    15901 gccgtccgtg aagtcgtcgt ccagcaggat ggcgctttgc tgggcgcggg cgtccagatc
    15961 ggcatccgtc gtcagccaga cgtcgggctg gaactggccg gagcggcccc gtcgcacgag
    16021 aaggtaggcg ccgtcggagc ggccgccggg gaaggggaga tccgggaagc ggtgtgcggc
    16081 gctgtcgctg ctccaggcct ggtggtcgtc tgcactgggt tcggcatagg ggttgaggct
    16141 gcaggcggtg tcgaagccgg tcgcgatcac gtcggcgagc atcgtggccg gtacgatcag
    16201 gcgcggcggg tggcgcaggg cggcgcccag caggcgacgg gcgcaggcct gcacctgatc
    16261 gtgcgtgagg ggggtctcgt ggggcaccag gaaggcgagg gggcaggcct gaccgaaggt
    16321 ccacaggaaa tcgtcgaggg acagggacgg cacgccgtca aggcccgtcg gccccgcgca
    16381 ttcgaggcga ccgtcgacgc tgtgcaggat ggtggcaagg ccgctcgcgc cgagggcaag
    16441 ggccgaccac cacggatcgg tgccggtgcc ggggcggccg gccccgtgcc gagtgtcgcc
    16501 accgatggca aggcagggaa gggagggtgt gggcatcgcg gggtgtctcc ggaggcgcga
    16561 aagggaatcc tgcctgtgca caggcgccct tcggcgacgg aggcccacac ccggtctgcg
    16621 cagaaggcgg cgtggcgcat cggcgcaggg cccggagatg ggcgtgctgc ataggcaggc
    16681 gcccattctg ggcgcctgcc tatgcgtttg tcgtcatcag aattggtgaa tgcgcccgat
    16741 ggcccacgac ggtccgggcc gggggctcag gggagtgcgg cgtcgagggt ccgcaggaag
    16801 gcgtcggcgg cctggaagcc gatgacacgc tgggcgcggc gttctgcgcc gtgggcgtcg
    16861 aaaaagagga tgcccggcgg gccgaagaga tcgaagcgct tgagcagggc cttgtcgtcc
    16921 tcgctgttgg cggtgacgtc ggcctgcagg aggaggacgc cggcgaggcg tcgggcgacg
    16981 gcggggtcgg cgaaggtgaa gcgttccatc tccttgcagg acacacacca gtccgcgtag
    17041 aagtcgagca tcaccgggcg gtcggcggtc ttgaggatct cgtccagctc ggcgccagag
    17101 gcgatgcggc ggaagtgcgg gccctcggcc ggcgcggcgg cctgggcgcg caggatggcc
    17161 aggggctgga ggggatcgcg ggagccggcc agggcgccga tgaacagcgc cgcgccggtg
    17221 agcagggcga ccacgccgat gcccttccaa aagcgctgcc agccggcggc atggggcggc
    17281 agcgggtcga gggcgtgcag gtagatggcc gacatgatga gcagtccggc ccaggccagc
    17341 atgggcacca ctgccggcag caccggcgag accagccaca ccgcgacgcc cagcagcatg
    17401 accccgaagg cccgcttgac gccttccatc cacgggccgg ggcgcggcag ggcgctgcgg
    17461 gcggctacac cgaccagcag caggggggcg cccatgccca gcgccatggt gaacagggcc
    17521 gcaccgccga gcagcgcgtc gccgctccgg gcgatgtaga ggagcgcgcc ggccaggggc
    17581 gcggccacgc aggggccgac gatcagggcc gacagggcgc ccatcaggat cacgccggcc
    17641 ggcgagccgc cgggctggtg attggcgcgg tcggacaggc ggctctgcag cgcggtgggc
    17701 agttgcagct cgtagaagcc gaacatggac agggacaggg ccacgaagac cagggcgaag
    17761 ccgcccagca cccagggatt ctgcagggcg gccgacagca gggtgccgga cagcccggcg
    17821 gcgacgccgg cggcggtgta ggtgagggcc atccccagca cgtagagcag cgacagggtg
    17881 aaggcccgcc cgcgggagat ggcgtggccg tggccgacga tgatgccgga gaggatcggg
    17941 atcatcggga a
//
LOCUS       DAOQPA010000074        17784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1594_length_17784_cov_16.421287, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000074 DAOQPA010000000
VERSION     DAOQPA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17784
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_17784_cov_16.421287"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKX01_11040"
     CDS             complement(<1..837)
                     /locus_tag="PKX01_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HMZ76707.1"
                     /translation="MRRRFPALLLTALLTVSGAARGAIDLADDQGTPVHLAQPARRIV
                     SLAPHVTELLFAAGAGGKVVGAVQYSDYPEAARSVARVGSYTAVDMEKVAALKPDLVV
                     AWKSGNRDAHLDKLRALGIPVFINEPRRIEDVARSLTQLGRLAGTEAAAEAAAQAFRA
                     RQGALAARYSARPTVRVFYEIWNHPLMTINGEHLISDVIRLCGGENVFARLPVLAPAI
                     DTEAVLAAAPEVIVASGMGEARPEWLDDWRRWTKLNAVARGNLFFIPPDQIQRHTPRI
                     LDG"
     gene            complement(846..1820)
                     /gene="cobD"
                     /locus_tag="PKX01_11045"
     CDS             complement(846..1820)
                     /gene="cobD"
                     /locus_tag="PKX01_11045"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HMZ76708.1"
                     /translation="MLEHGGRLREAARRTGIARADWLDLSTGINPHGYPVPSLAAEAW
                     QRLPEVDDGLEAAAAAYYGSAELLPVAGSQAALQALPACFPPGRVLTLAPTYAEHPHA
                     WRHHAVTALPAAALDDDAVDRTDTLLLVQPNNPDGQRFPRTRLLAWHARLQARGGRLI
                     VDEAFADADPVDSLTPLAGTPGLVVLRSLGKFFGLAGARVGFVFAEAALRDALAERLG
                     PWTVAGPARAVATAALQDRAWQAATRHRLAADSRRLAGLLAAHGLPPAAGTALFQWVP
                     HGDAAQIHEALARQGILLRLFTAPTGLRFGLPGTPAHWDRLAAALARL"
     gene            complement(1813..2766)
                     /gene="cbiB"
                     /locus_tag="PKX01_11050"
     CDS             complement(1813..2766)
                     /gene="cbiB"
                     /locus_tag="PKX01_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HMZ76709.1"
                     /translation="MPGFTLPVATSPVALPLALLMALGLLLDRLLGEPRHHHPLVAFG
                     RIAGRIESLLRLTGSGRAAGLLAWALAVLPWVALAGSLRPLHPWAGALLDATLLYLAV
                     GGRSLAEHAAAVATPLLDGDLGGARQRLSWMVSRDTTQLDESGVANATVESVLENGND
                     AVFGALFWFVLLGGPGALLYRLANTLDAMWGYRNARYLRFGWAAARIDDVLNYLPARL
                     TALSYALLGHTATALRCWRTQAPAWDSPNAGPVMASGAGALDLRLGGPAVYHGHLETR
                     PALGDGARARAADIDRAVALVQNTTWLWLGVIAAAGFALRA"
     gene            2806..4317
                     /locus_tag="PKX01_11055"
     CDS             2806..4317
                     /locus_tag="PKX01_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HMZ76710.1"
                     /translation="MRRASTLMVQGTTSDAGKSTLVAGLARLLRRRGVAVAPFKPQNM
                     ALNSAVTADGGEIGRAQALQALAAGVPPHTDMNPVLLKPASDIGAQVIIHGRAVAHLS
                     ARDYHDFKPRAMAAVLASFGRLCAGYDAVLIEGAGSPAEINLRDRDIANMGFAEAADC
                     PVILVADIDRGGVFAHLVGTLDLLSASEQARVKGFVINRFRGDIGLLQSGLDWLEART
                     GRPVLGVLPYLHGLFLDAEDALGEACVDKSATRLRVVAPVYPRISNHTDLDALRLHPQ
                     VDFRWVGPGQAMPPADLIVLPGSKAVQADLAWLRAQGWEPALRRHLRYGGKLVAVCGG
                     FQMLGTRLHDPQGLEGEAGSVAGLGLLDAETVLEAEKQLVNVHGRLALPGAAPVSGYE
                     IHMGVTRGPALAQAAVELEDGRRDGAISDDGQILGTYLHGLFDHPDALVALLAWAGAG
                     DVDRVDLAARREADLERLADALEAHLDLPRLAACLGDGTAAEGVRTSLHVPLA"
     gene            4350..4544
                     /locus_tag="PKX01_11060"
     CDS             4350..4544
                     /locus_tag="PKX01_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76711.1"
                     /translation="MDAEFASLEQKLEQLLALHAAAQAENRSLRERLAAVEGANRELN
                     DRLGIAGSRLEALLARIPEV"
     gene            4548..4844
                     /locus_tag="PKX01_11065"
     CDS             4548..4844
                     /locus_tag="PKX01_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMZ76712.1"
                     /translation="MEHLDIKLLGREYRVACKPEERDGLMAAVAYLDEKMRDLGAKTN
                     SAGERLAVMTALNITHEFLQFQRAGGFDIQAVKRRIEHMNSRLDGVLAPQEKLF"
     gene            complement(5201..5656)
                     /locus_tag="PKX01_11070"
     CDS             complement(5201..5656)
                     /locus_tag="PKX01_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ76713.1"
                     /translation="MSDLLRSAPARHPVRLVLTLGVAALATQMAGCGNKAAVDEELAA
                     KLTQPVASVVLTAAPAAAPGSRSGEDIYKAVCSACHASGAAGAPKAGDKAAWGPRLGA
                     GLAGLVKSATNGKNAMPPKGGAADLTDTELTRAVAFLANQAGAGFKEPQ"
     gene            5803..5879
                     /locus_tag="PKX01_11075"
     tRNA            5803..5879
                     /locus_tag="PKX01_11075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5837..5839,aa:Arg,seq:ccg)
     gene            complement(6025..6438)
                     /locus_tag="PKX01_11080"
     CDS             complement(6025..6438)
                     /locus_tag="PKX01_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76714.1"
                     /translation="MPAPAHTDFDIALIEDSTVLRDLLSAMLADIPNIRLVGAAASEA
                     EGLKLLQATRPRLVIVDLELREGTGLRVLAALQRQPDHFGSPQAVVFSNHAHSVVQAR
                     CRMLGARAFFDKSFQTDDLLAFIEDAAGCPATASH"
     gene            complement(6698..6964)
                     /locus_tag="PKX01_11085"
     CDS             complement(6698..6964)
                     /locus_tag="PKX01_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76715.1"
                     /translation="MRGIQNTPQEPKWALPGKWDWSVRLSLVSSVALCAGMLYGSSVG
                     HQGARPRLESTSCLPDRHDVDRLIGPAAWTSVTDCVLLPRDTSH"
     gene            complement(7293..7604)
                     /locus_tag="PKX01_11090"
     CDS             complement(7293..7604)
                     /locus_tag="PKX01_11090"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76716.1"
                     /translation="MSATHSTANSLRQIANDLGDSARDALPPVRKQLGITADTTREAA
                     TSLMGNLRTAARDTVGIVRRDAAHASEAAGAYIRRKPLQSAAVVGVVGLLIGALLGRR
                     F"
     gene            complement(7661..8005)
                     /locus_tag="PKX01_11095"
     CDS             complement(7661..8005)
                     /locus_tag="PKX01_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HMZ76717.1"
                     /translation="MKTPLPGILLASLLALALASVPATQAATAAQGDRQAPRSAAARF
                     IDDASITANIKARHAEDRSVRITAIGVETRNGVVQLSGFTPSPAEKTRAEALAWTIPG
                     VKAVQNDIILIN"
     gene            8332..10254
                     /locus_tag="PKX01_11100"
     CDS             8332..10254
                     /locus_tag="PKX01_11100"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HMZ76718.1"
                     /translation="MNMPISPRSRRRLHHVAAILAVAGLGVALFDGNLLRGPLARLAS
                     AQLGRSVQIAGPVDLHPWSLHPTLSVGGVTVANVTGGRASLLASAGRLAVTADLRALL
                     RGAVVLDALEVDRAEIHLEEDREGRGNWQLGGQIGAGGKDPAAAPTRLRIARVRIHDS
                     RLSAELPSRKTSLQLEVATGDDGRIRFTARGRYQGQDLTVDGEAGSVPGLIDGQLPYP
                     LKAAGTVGATRFRIDGSSADLAGLNGLDVGFELAGRSLAELYPLTGVPLPASPAYRFA
                     ARLVQSGTLWRFENIDGTVGRSDLAGTFAVDRGPVPQRLSGTLRARHLDLQDLSGFLG
                     ARDSSGERLAPRPGKVLPARPLGFDKIAAADVDLDFAIGDIRHSGLPLDAASGHLRIV
                     DRRVLLQPLRVGMAQGEVSGVLELETRGMPARARLDLQASRLKLRELMPTLESRSLTT
                     GVLGGRATVSMRGNSVAALLGSATGDLALAMDGGSTSRLLVRLANLDVANAFVSWLAG
                     GQREDIRCLVGDFSARDGVLEPRALLLDTERTLIRGEGQINLRDETLDLRLRASARDG
                     SLLSLRGPLHLDGSFADPKVLPEPVPLGSRVAGAVALGLISPPLALLPLLEAGDARES
                     ACKRLLQQAGKTMGRP"
     gene            complement(10371..10538)
                     /locus_tag="PKX01_11105"
     CDS             complement(10371..10538)
                     /locus_tag="PKX01_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HMZ76719.1"
                     /translation="MLHYAVVFFVIAIVAALFGFGGIAAGAVEIAKVLFFIFLILAIV
                     SFVFNLLLGKG"
     gene            complement(10594..10797)
                     /locus_tag="PKX01_11110"
     CDS             complement(10594..10797)
                     /locus_tag="PKX01_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HMZ76720.1"
                     /translation="MNHNIIEGNWKQLKGKLKEHWGKLTDDDLDVIEGRREVLAGRIQ
                     ERYGLTLDEAKRHVQAWEKMLDA"
     gene            11179..11361
                     /locus_tag="PKX01_11115"
     CDS             11179..11361
                     /locus_tag="PKX01_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76721.1"
                     /translation="MKRVSIDWNAKPVRAARRSPARSVWTDTLEDEIDLRRLRTERRL
                     DKVRRALINQSGLTSR"
     gene            11432..12826
                     /locus_tag="PKX01_11120"
     CDS             11432..12826
                     /locus_tag="PKX01_11120"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HMZ76722.1"
                     /translation="MNTVLPLRDPRARMRLVRHWPVFLVILGVGLLLGLLTIGRLHSE
                     FGVSALRTSQAYNSQIDRLNRLLIVLLDAETGVRGFIASGLNPVYLEPYQQAEQDIGP
                     LLKAISNDYPQGTQDHTEFELLGQLIAVKRQLLAETVRIRRVPTVVPPGETGPGKAYM
                     DQIRLSIAVLRGRLEERNARSIAESTKRFNDTRTLVTVLALGAVGLVLALFAVQQRQT
                     VLRARIAELLERENIALESTVAERTRELSDLASYLTTARELEQARLARELHDELGALL
                     TAAKMDASWLLRSLGAQSTPDIQARFRRLIDTIGSGITIKRRVVSDLRPPLLQGLGLV
                     EALRALSDDLHDEYRVTLQLPGENIDCDEEQALALFRIVQESVTNIRKYARARAIEVG
                     LAVDAGVVRLWVKDDGVGFDPDNPQLNRHGLAGMKHRVQMFEGRFTVTSAPGAGTRID
                     ATMPLRPAAPSAHG"
     gene            complement(12889..13524)
                     /locus_tag="PKX01_11125"
     CDS             complement(12889..13524)
                     /locus_tag="PKX01_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76723.1"
                     /translation="MLRLAIVDDHKIVRAGFREMLSDELGIQVEFEAASGEEALQHLR
                     TTPCDVLLLDLSLPGQSGVDVLRAVRQRYADLKVLVLSGFPEERYALPMIRNGANGYL
                     CKDCERDELIRAIRTVAQGRRYVSPRTAELLADDLAGAGTALPHESLSERELQVFLRL
                     ARGESVSGIADVLNLSVKTVSTYRSRLLEKLDVASNAELAAYALRHGLIVD"
     gene            13763..14173
                     /locus_tag="PKX01_11130"
     CDS             13763..14173
                     /locus_tag="PKX01_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HMZ76724.1"
                     /translation="MKIVRRRPLALEASVRIGLLAVTFVAAFAAGHSTASRAPQSLDD
                     PAAGPALLRDGVDRAGRSVAAAVGLDVYSEAGRPVGRLTALLHGEGHDGLARIAVAER
                     GDEGAREIVVPLGRLVWRDDRVVLADGAAPRWLI"
     gene            14184..15377
                     /locus_tag="PKX01_11135"
     CDS             14184..15377
                     /locus_tag="PKX01_11135"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HMZ76725.1"
                     /translation="MATAAARPQDRATRLKVRRARKMARSAHAYVRGSTARFYAWLAA
                     SDGDALPDGPPVWICGDCHLGNLGPVADSDGRTGVEIRDLDQAVIGNPAHDLVRLGLS
                     LACACRSADLPGVMSLRVVDMLLDGYARAFAQRPRPPSPPDSVQAVIRRAVKRTWKQL
                     MRDHLDHARPRIPLGARFWPLTRREQGAIAQCLDETRLPELARQLGIARDTEAVRVAD
                     AAYWVKGCSSLGRPRYVVLLQVDGAAEARLAMVDIKDAPPAAAPRTGSVSMPRDNAER
                     VVEAARALAPALGGRMVAMRLLDRPMVARELSPQDLKIEPDQLAPADMAALAHYLGRI
                     VGDAHARQMSAPQRRHWHATLRRSHSRVVDAPSWLWRAIVDALADHERGYLEHCRRHV
                     AALVA"
     gene            15555..15950
                     /locus_tag="PKX01_11140"
     CDS             15555..15950
                     /locus_tag="PKX01_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76726.1"
                     /translation="MPSEPTVLRTVIIEDNAVLLDLLTGMLAGIEGVSVVGHAASEAE
                     ALALVRATRPQLAIVDLELRGSSGLGVLAAFQQTPDEYGAPLAIVFSNHAHPIVQSRC
                     LALGARAFFDKSFQMDDLLDFVQATARAL"
     gene            complement(16046..16357)
                     /locus_tag="PKX01_11145"
     CDS             complement(16046..16357)
                     /locus_tag="PKX01_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HMZ76727.1"
                     /translation="MKTTAVTLALAGLLAFSAAGCAITREQSTVGQYVDDATITTRVK
                     AKFAEDSTVSAMAIGVETLKGTVQLSGFAKSTTERDRAESLARGTPGVSMVKNDIVVR
                     P"
     gene            16748..>17784
                     /locus_tag="PKX01_11150"
     CDS             16748..>17784
                     /locus_tag="PKX01_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene monooxygenase"
                     /protein_id="HMZ76728.1"
                     /translation="MALLNRMDWYDLARSTNWTPKYVTEDELFPPKLSGEFGLPLTAW
                     ESYDEPYKQTYPEYVKVQREKDAGAYSVKAALERSRIFENADPGWKSVMKAHYGAIAR
                     GEYAAASAEARMMRFSKAPGMRNMATLGCMDEIRHGQMQLYFPHEHVAKDRQMDWAFK
                     AYDTNDWAMIAARHFFDDIMMTRDAISVSIMLTFSFETGFTNMQFLGLAADAAEAGDH
                     TFANLISSIQTDESRHAQIGGPALKVLIENGQKAEAQKRVDIAVWGAWKLFSVLTGPI
                     MDYYTPLEHRKQSFKEFMEEWIVAQFERALTDMGLELPWYWEIFLKDLSETHHGMHLG
                     SYYWRPTVWWNP"
BASE COUNT         2589 a         5946 c         6562 g         2687 t
ORIGIN      
        1 gccgtcgagg atgcgcggcg tgtggcgctg gatctggtcc ggcgggatga agaacaggtt
       61 gccccgggcc actgcgttga gcttggtcca gcgccgccag tcgtcgagcc actccggccg
      121 tgcctcgccc atgccgctgg cgacgatgac ctccggcgcc gcggcgagca ccgcttcggt
      181 gtcgatggcg ggcgccagca cgggcagccg cgcgaacacg ttctcgccgc cgcacaggcg
      241 gatcacgtcg gagatgaggt gctcgccgtt gatggtcatc agcgggtggt tccagatctc
      301 gtagaacacg cgcaccgtcg gccgcgccga atagcgggcc gccagggcgc cctggcgggc
      361 gcggaaggcc tgcgcggcgg cctccgcggc ggcttcggtg ccggccaggc ggccgagctg
      421 ggtcaggctg cgggccacgt cctcgatgcg ccgcggctcg ttgatgaaga ccgggatgcc
      481 gagggcgcgc agcttgtcga gatgggcgtc ccggttgccg ctcttccagg ccaccaccag
      541 gtcgggcttg agggcggcga ccttttccat gtccaccgcg gtgtagctgc ccacccgcgc
      601 caccgaccgg gcggcctcgg ggtagtcgct gtactgcacc gcgcccacga ccttgccgcc
      661 ggcgccagcg gcaaaaagca gctcggtgac gtggggcgcc agactgacga tgcgccgtgc
      721 cggctgggcc aggtgcaccg gcgtgccctg gtcgtcggcg aggtcgatgg cgccgcgggc
      781 ggcgccggag acggtgagca gggcggtgag cagcagggcg gggaagcgtc ggcgcatggc
      841 gggggtcaga gtcgggcgag ggccgcggcg aggcggtccc agtgggcagg tgtacccggc
      901 aggccgaagc gcaggccggt cggcgcggtg aagaggcgaa gcaggatacc ttggcgggcc
      961 agggcctcgt ggatttgggc tgcatcgcca tggggcaccc actggaacag ggcggtgcca
     1021 gcggcgggag gcagcccgtg ggcggcgagc aacccggcca gacgccggct gtcggcggcc
     1081 aggcggtggc gggtggcggc ctgccaggcg cgatcctgca gggcggccgt ggcgacggcg
     1141 cgggcgggcc cggcgacggt ccacggtccg aggcgctcgg ccagggcgtc gcgcagggct
     1201 gcctcggcga acacgaagcc gacccgcgcg ccggccaggc cgaagaattt gcccagggag
     1261 cgcagcacca ccaggccggg cgtgccggca aggggcgtca ggctgtcgac cgggtcggca
     1321 tcggcgaagg cttcgtccac gatcaggcgg ccgccgcggg cctgcaggcg ggcgtgccag
     1381 gcgagcaggc gggtgcgggg aaagcgctgg ccatccgggt tgttgggctg gacgaggagg
     1441 agggtgtccg tacggtcgac ggcgtcgtcg tcgagggccg cggcgggcag cgccgtgacc
     1501 gcgtggtggc gccaggcgtg gggatgctcc gcgtaggtgg gcgcgagggt cagcacccgc
     1561 cccggcggaa agcacgccgg cagggcctgg agggcggcct gggagccggc caccgggagc
     1621 agctcggcgc tgccgtaata cgccgcggca gcggcttcga ggccgtcgtc gacttccggc
     1681 aggcgctgcc acgcctcggc ggcgagggac ggcaccgggt agccgtgggg attgatgccg
     1741 gtggacaggt cgagccagtc ggctcgcgcg atgccggtgc ggcgcgccgc ctcgcgcagc
     1801 cggccgccgt gttcaagcac ggagggcgaa tccggccgcg gcgatcacgc cgagccacag
     1861 ccaggtggtg ttctgcacga gggccacggc ccggtcgatg tcggcggcgc gggcacgggc
     1921 gccgtcaccg agggccgggc gggtctccag atggccgtga tagaccgccg ggccgcccag
     1981 gcgcaggtcc agggcgccgg cgcctgaggc catcaccggg ccggcgttgg ggctgtccca
     2041 ggcgggcgcc tgggtgcgcc agcagcgcag ggcggtggca gtgtgcccga gcagggcgta
     2101 ggacagggcg gtgaggcggg ccggcagata attgagcacg tcgtcgatgc gcgcggcggc
     2161 ccagccgaag cgcaggtagc gcgcgttgcg gtagccccac atggcgtcca gggtgttcgc
     2221 caggcggtac agcagggcgc ccggcccgcc cagcaggacg aaccagaaga gggcgccgaa
     2281 caccgcgtcg ttgccgttct cgaggaccga ttcgacggtg gcgttggcca cgccggattc
     2341 gtcgagctgg gtggtgtcgc gggagaccat ccaggacagg cgctggcgcg cgccgcccag
     2401 gtcgccatcg agcaggggcg tcgccacggc cgcagcgtgc tcggcgaggc tgcgtccgcc
     2461 gacggcgaga tacagcaggg tggcgtccag cagggcgccc gcccagggat ggagcggccg
     2521 cagcgagccc gccagcgcga cccagggcag caccgccagc gcccaggcca gcaggcccgc
     2581 ggcgcgaccg gagccggtga ggcgcaacag gctttcgatg cggccggcaa tgcggccaaa
     2641 ggcgacaagg gggtgatggt gccggggttc gccgaggagg cggtcgagga gcagaccgag
     2701 ggccatcaac agggccaggg gaagcgccac gggtgacgtg gcgaccggga gggtgaagcc
     2761 gggcatggga taatcgacaa cttttcgcag acgggattgt aagccatgcg acgcgcgtcc
     2821 accctgatgg ttcagggcac cacctccgat gcgggcaaga gcaccctggt ggcgggcctg
     2881 gcccgcctgc tgcgccgccg tggcgtggcg gtcgcgccgt tcaagcccca gaacatggcc
     2941 ctcaactcag cggtgacggc ggacggcggc gagattggtc gcgcccaggc gctgcaggcg
     3001 ctggctgccg gcgtcccgcc ccatacggac atgaacccgg tgctcctcaa acccgccagc
     3061 gacatcggcg cccaggtgat catccatggc cgtgccgtgg cccatctgtc ggcgcgggat
     3121 tatcacgact tcaagccgcg ggcgatggcc gcggtgctgg cgagctttgg ccgcctctgc
     3181 gcgggctacg acgcggtcct catcgaaggg gccggcagcc cggcggagat caacctgcgc
     3241 gaccgggaca tcgccaacat gggcttcgcc gaggccgccg actgcccggt gatccttgtc
     3301 gcagacatcg accggggcgg cgtcttcgcg cacctggtgg gcaccctgga cctcctctcg
     3361 gccagcgagc aggcgcgggt gaagggtttt gtcatcaacc gcttccgtgg cgacatcggc
     3421 ctgctccagt ccgggctgga ctggctcgag gcgcgcaccg ggcgcccggt gctgggcgtg
     3481 ctcccctatc tgcacggcct cttcctggat gccgaggatg ccctcggcga ggcctgtgtc
     3541 gacaagtcgg ccacgcgcct gcgggtcgtc gcgccggtgt acccgcgcat ctccaaccac
     3601 accgacctgg acgccctgcg cctgcatccc caggtggact tccgctgggt cgggccgggc
     3661 caggcaatgc cccccgccga cctgatcgtg ctgcccggct ccaaggccgt gcaggccgat
     3721 ctggcctggc tgcgcgccca ggggtgggag cccgccctgc gccgccacct gcgctatggc
     3781 ggcaagctcg tggcggtgtg tggcggcttt cagatgctcg gaacccggct gcacgacccg
     3841 cagggcctgg agggcgaggc gggcagcgtc gccggcctcg gactgctcga tgcagagacc
     3901 gtgctcgaag cggagaagca gctggtgaac gtccacggtc ggctcgccct gcccggcgcc
     3961 gcgccggtga gcggctacga gatccacatg ggcgtgaccc gcggtccggc cctggcgcag
     4021 gcggccgtgg agctggagga cgggcgccgg gacggtgcca tctcggacga tgggcagatc
     4081 ctcggcacct acctgcacgg gctcttcgat cacccggacg cactggtggc gctgctggcc
     4141 tgggcggggg cgggcgacgt ggatcgggtg gatctggcgg ctcgacgcga ggccgatctg
     4201 gagcggctgg ccgacgcgct cgaggcccat ctggatctgc cgcggctggc cgcatgcctg
     4261 ggtgacggga cggccgccga gggcgtgcgt acgtcattgc atgtgcccct tgcttgaccg
     4321 cgtttcgggc cctctctaac attcgccgca tggacgccga attcgcctct cttgaacaga
     4381 agctcgagca gcttctcgcc ctgcatgccg ccgcacaggc ggaaaaccgc agtctgcggg
     4441 agcggctggc tgccgtcgaa ggcgccaacc gcgagctgaa cgaccggctg ggtatcgccg
     4501 gatcccgcct cgaagccctt ctcgcacgca tccccgaggt ctgatgcatg gaacacctgg
     4561 atatcaaact cctcggccgc gaatatcgcg tggcctgcaa gcccgaagag cgggacggcc
     4621 tgatggcggc ggtggcctat ctggacgaaa agatgcgcga tctgggtgcc aagaccaact
     4681 cggccggcga gcgtctggcg gtgatgacgg ccctcaacat cacccatgaa tttctgcagt
     4741 tccagcgcgc cggcggattt gacatccagg cggtcaagcg tagaatcgag cacatgaaca
     4801 gtcgccttga tggcgttctg gcgcctcagg aaaagctctt ctgaggcaac cggaagcgtt
     4861 gttcaatgtt ttgatttgca agggttttca ggttcgatca tcccctgcgg tgttggtcaa
     4921 ggctgtatac tccttgaacc aatgtcttta tgactgggtc gtggcccgta gaaaccactg
     4981 gtgcgcgcgc cccactgtcg ggtgtgcctg aagtgggagg aaagcgaccc ccctgaaccc
     5041 cggttcagga tgccggcctg accgcacagg cgggggacct acaaaaaaag cgcagagttt
     5101 cggctctgcg ctttttcttt gtctgagcac gcgggcaact ggggctgccg cgcgcttcgg
     5161 tggcctgttc cacaggaaac aggccgccgg cagagactgc ttattgcggt tccttgaaac
     5221 cggcgcccgc ctggttggcg aggaaggcca cggcgcgggt cagttcggtg tcggtcagat
     5281 ccgccgcgcc acccttgggc ggcatggcgt tcttgccgtt ggtggcggac ttcaccagac
     5341 cggccagacc ggcgcccaga cgcggacccc aggcggcctt gtcgccggcc ttgggcgcgc
     5401 cagcggcgcc gctggcgtga caggcgctgc acacggcctt gtagatgtct tcgccgctac
     5461 ggctgcccgg cgcggcggcc ggcgcagcgg tcaggaccac gctggccacg ggctgggtca
     5521 gcttggcagc cagttcttca tcgacggcgg ccttgttgcc gcagccggcc atctgggtgg
     5581 ccagcgcagc gacgccaagg gtgagaacaa gccgcaccgg gtggcgggcg ggagcagagc
     5641 gaagcagatc cgacatgctg aaatcctcga gagcgggggg acgattgatt aaagcgttga
     5701 ttatagcggg ggtattcccc ttctgtcagg cgaagcatgg acgccggcgg cgattcgcgc
     5761 tatcctgcgc gccgcgtgac gtctccggcg tcattgctcc gcgcgcccgt agctcagctg
     5821 gatagagtac tgccctccga aggcaggggt cggacgttcg aatcgtctcg ggcgcgccat
     5881 tctctcaatg ggttggatcg aaagatccgg cccattgtcg tttccggcgt ccgatcccgc
     5941 ggcgcgggct gcccgcgcgg atgcccgtct ccgaacgccg cgtgaagccg tcggtcccgt
     6001 tctgctcgtg gcaggggtcc ttgatcaatg gcttgcggtg gcagggcagc ctgcggcgtc
     6061 ttcgatgaag gcgagcagat cgtccgtctg gaaggacttg tcgaagaagg cgcgtgcgcc
     6121 cagcatccgg cagcgggcct ggacgacgga gtgggcgtgg ttggagaaga ccaccgcctg
     6181 tggcgagccg aagtggtccg gctggcgctg cagcgccgcc agcacacgga gtccggtgcc
     6241 ctcccgcagt tccagatcga cgatgacgag ccgggggcgg gtggcctgaa gcagcttcag
     6301 gccttcggcc tcgctggcgg cggcaccgac gaggcggatg ttcgggatgt cggccagcat
     6361 ggccgacagc aggtcgcgca ggacggtgga gtcttcgatc agtgcgatgt cgaaatcggt
     6421 gtgggcaggg gcaggcatgg gggttctcgt gcggcggagg tgttcagttc gggcgtggcg
     6481 catgggtcgg cgcgaaggcg aaagtgtccg ggagggtctt tgacgtccag gttgagcgtt
     6541 cagccgggcg gtcggcttcg gccgacgggt cacgcacgaa gcgcagtagc gccccggtgg
     6601 gggcccctca ggccatgacg acccggcccg ccacacaaca tccccgcggg ccggtccgtg
     6661 tctggggcgc cggcatgggt gagatagacc gtcggcgtca atgggaggtg tctctgggca
     6721 gcaggacgca atcggtgacg gaggtccagg ccgcgggtcc gatcaggcga tcgacgtcgt
     6781 gccggtcggg caggcatgag gtgctctcca ggcgcggccg cgcgccctga tggccgacgg
     6841 acgacccgta cagcatgccg gcgcagaggg cgacggagct gacgagggac agtcgcaccg
     6901 accagtccca tttgccgggg agcgcccact tgggttcttg cggggtgttc tggatgccgc
     6961 gcatgttcat ctcctgaggg ggtggttcag gtgccgggat catcggtggc cacgccggca
     7021 cgcggggtcg gatctgtgtc cgatcggctg tcctgctggc gtcccgttcg gccgaaaccg
     7081 gatcggtgac gccgcacgcc cttcgaggcg aagctggcca ctgcgatctg accggaccgc
     7141 aacgcagggg ttgcagctgc actgcgtaaa gtcatgacaa gcggtgtaaa gaaggccgcc
     7201 ggcggagcgg acagggcacc gccgggcggg cccttgcggg gccggggcac gggtcgggcg
     7261 tgggcgacat ggaggccgtg tcccggcgtc gttcagaacc ggcggcccag gagggcgcca
     7321 atcagcagac cgaccacccc gacgacggcg gccgactgca gcggcttgcg gcggatgtag
     7381 gcgcctgcgg cctcgctggc gtgggcggcg tcgcggcgga cgatgcccac ggtgtcgcgg
     7441 gcggccgtgc gcagattgcc catcaggctg gtggccgcct cgcgggtggt gtcggcggtg
     7501 attccgagct gcttgcggac cgggggcagg gcgtcgcggg cgctgtctcc caggtcgttg
     7561 gcgatctggc gcaggctgtt ggcagtggaa tgggtggcgc tcatggtggt gctccttggg
     7621 gtggggatcg ggggcgggcg gaggtgtcgc gaccgcgcaa tcagttgatg aggatgatgt
     7681 cgttctggac cgccttcacg ccggggatcg tccaggcgag tgcctcggcg cgggttttct
     7741 cggcggggga gggggtgaag ccggacagct gcacgacgcc gttgcgggtt tcgacgccga
     7801 tggcggtgat gcgtaccgac cggtcttccg cgtgcctggc cttgatgttc gcggtgatgg
     7861 aggcgtcgtc gatgaagcgg gcggccgccg agcggggggc ctggcggtcg ccctgggccg
     7921 cggtggcggc ctgggtggcg ggtacgctgg cgagcgccag ggcgaggagg ctggcgagga
     7981 ggatgccggg gaggggtgtc ttcacggtgg ggtctccttg tggaccggaa tagccgggtt
     8041 cggggcgggc accggaccgg gggtccgaca ccgttctgcc atgactgcac tgtacggaag
     8101 gccgggcggg cggactgtcc gacaagcgcg gatgcgtgtg taggaatcgg ccgtcggaca
     8161 tgtcggcgct ggccgggccg gggacgccgc cggcgggccg cgtggtcgtg ccccgtggcg
     8221 gcaggggcac ggcgcggtgc aggaaaattc cgacgcgggg ctcatccgcc accgacaccg
     8281 gggcgggcgc gacggaccta gaatgggtcc ggacccttac acgcttccat catgaacatg
     8341 ccgatctctc cgcgctcccg ccgtcgcctg caccatgtgg cggccatcct cgccgtggcg
     8401 gggctgggtg tcgcgctgtt cgacgggaac ctgctgcgcg gccccctggc ccgcttggcc
     8461 agcgcgcagc tcggtcgcag cgtccagatc gcaggcccgg tcgatctgca tccgtggagc
     8521 cttcacccga ccctcagtgt gggcggcgtg accgtggcga acgtgaccgg cggccgcgcc
     8581 agcctcctgg cgagcgccgg ccggctggcc gtgacggccg acctgcgggc gctgctgcgg
     8641 ggggcggtgg tgctggatgc gctggaggtc gaccgggccg agatccacct ggaagaagac
     8701 cgggaggggc gcggcaactg gcagctcggc gggcagatcg gcgcgggcgg caaggacccc
     8761 gctgcggcgc cgacgcgcct gcgcatcgcc cgggtgcgca tccacgacag ccggttgagc
     8821 gccgagctgc cgtcgcgcaa gacctcgctg cagctcgagg tggcgacagg ggacgacggt
     8881 cgcatccgct tcaccgcccg tggtcgctac caggggcagg acctgacggt ggacggcgag
     8941 gcggggagcg tgcccgggct gatcgacggc cagctgccct acccgctgaa ggccgccggc
     9001 acggtgggcg ccacgcgctt ccgtatcgac gggtcgagcg ccgatctggc agggctgaac
     9061 ggcctcgacg tgggcttcga gctggcgggc cgcagtctgg cggagctgta tccgctgacc
     9121 ggcgtgccgc tgccggcgtc tccggcctac cggttcgcgg cgcggctggt gcagtccggc
     9181 accctgtggc gcttcgagaa catcgacggc accgtcggcc gcagcgatct ggcgggcacc
     9241 tttgccgtgg atcgggggcc cgtgccgcag cgcctgagcg gcacgctgcg cgcccggcat
     9301 ctggatctgc aggatctctc ggggttcctc ggcgcgcgcg acagcagcgg cgagcggctg
     9361 gcgccccggc cgggcaaggt gttgccggca cgcccgctgg gattcgacaa gatcgctgcg
     9421 gccgatgtgg atctggactt cgccattggc gacatccgcc acagcggcct gcccctcgat
     9481 gccgcgtcgg ggcatctgcg catcgtcgat aggcgtgtcc tgctccagcc cctgcgcgtc
     9541 ggcatggccc agggcgaggt gagcggcgtc ctcgagctcg agacccgcgg catgcccgcc
     9601 cgtgcgcgtc tggatctgca ggccagccgg ctgaagctgc gtgagctgat gcccacgctc
     9661 gaatccagat ccctgaccac cggcgtcctc gggggccggg ccacggtgtc catgcggggc
     9721 aattccgtcg ctgcgctgct gggcagcgcc accggcgacc tcgccctcgc catggatggc
     9781 ggcagcacca gccgcctgct ggtgcgcctg gccaatctgg atgtggccaa cgcctttgtg
     9841 tcctggctgg ccggcggtca gcgggaggac atccgctgtc tggtgggaga tttcagcgcc
     9901 cgcgacgggg tgctcgagcc gcgcgcgctg ttgctcgaca cggagcgcac cctcatccgc
     9961 ggcgaagggc agatcaatct gcgcgatgaa acgctggatc tccgcctgcg ggcgtcggca
    10021 cgggacggca gcctgctgtc cctgcggggg cctctgcatc tggacggaag ttttgccgac
    10081 cccaaggttc tgccggagcc ggtcccgctg ggcagccggg tggccggggc ggtggcgctc
    10141 gggctgatct cgccgccgct ggccctgctg cccctgctgg aagccggcga tgcacgcgag
    10201 tcggcctgca agcgtctcct ccagcaggcc gggaagacga tggggcgacc ctgaggggcc
    10261 gcagcagatc ggggcgtgaa cgggcgccgg gccccgcggg acgacgttgt gccgtcttcc
    10321 ggaggggccc ggcggaagtg tggcgggcgc tcatcgccgc ccgcgcgcat tcatcccttg
    10381 cccagcagca ggttgaagac aaagctgacg atggcaagga tcaggaagat gaagaacagg
    10441 accttcgcga tttccaccgc gccggcggca atcccgccaa aaccgaacag ggcggcgacg
    10501 atcgcgatga caaaaaagac cacggcatag tgcagcatgg cggttctcct gaaacggtga
    10561 tgacggacgg gggcgatggc gcggcgcggg ggctcaggcg tcgagcatct tttcccaggc
    10621 ctgcacgtgg cgcttggctt cgtccagggt gaggccatag cgctcctgga tccggccggc
    10681 gagtacctcg cggcggcctt cgatgacgtc caggtcgtcg tcggtgagct tgccccagtg
    10741 ttccttgagc ttgcccttga gctgcttcca gttgccttcg atgatgttgt ggttcatggc
    10801 gattctcctg aggttgaggg cttcgagcgt tccggagggg tgtcccgggc ggtcggcatg
    10861 cggtggagcc tgcatgactc cagcttaggc aggcgccgcc gggcgggcag tcggctggat
    10921 ctgaacggga tgtgggactg cgccgacggc cctgtcgggc atcgccgtcc ggggtgtccc
    10981 ggtggcgccc ggcggggccc gccggggcgg cggagcgcgg ccccgcagcg gcgggaaggc
    11041 gtgtcagaca actcccacat accattcagc gccgaccgac ggtatcgtga tgtttgtcac
    11101 ggtacagttt caaaaaaggc gttttataca gcgcccccac ccggcagcac gcccccgacc
    11161 catcgacagg agcacaggat gaaacgcgtc agcatcgact ggaatgccaa acccgtccgc
    11221 gcagcccgcc ggagccctgc gcgtagtgtg tggaccgaca ccctcgagga cgagatcgat
    11281 ctgcgcaggc tgcggaccga acgcagactc gacaaggtgc gccgcgccct catcaaccag
    11341 agcggcctga cgtcccgctg atcggcaggc cgccccccga cggggcgggg ccgcccgcct
    11401 gtccgcaatg ccccgcccgt tccccgccgc catgaatacc gtcctgcccc tgcgggatcc
    11461 ccgggcccgc atgcgcctcg tgcggcattg gcccgtcttc ctggtgatcc tgggggtcgg
    11521 gctgctcctc gggctgctca ccatcggccg gctccactcg gagttcggcg tgagcgccct
    11581 gcgcacctcg caggcctaca acagtcagat cgaccggctg aaccggctgc tgatcgtgct
    11641 gctggatgcc gagaccggcg tgcgcggatt catcgcgagc gggctcaatc cggtctatct
    11701 cgagccctac cagcaggccg aacaggacat cggccccttg ctgaaagcca tctccaatga
    11761 ctatccccag ggcacccagg accacaccga gtttgagctg ctcgggcagc tgatcgccgt
    11821 caagcgccag ctgctggcgg aaacggtccg catccggcgt gtgccgacgg tcgtgccgcc
    11881 gggagagacc gggccgggca aggcctacat ggaccagatc cgcctttcca tcgccgtgct
    11941 gcgggggcgt ctggaagagc gcaatgcccg ctcgattgcc gaatccacca agcgcttcaa
    12001 cgacacgcgc accctcgtca ccgtactggc cctgggtgcg gtggggctgg tgctggccct
    12061 gtttgccgtg cagcaacggc agaccgtcct gcgggcgcgc attgccgagc tgctggagcg
    12121 ggagaacatc gctctggaat ccacggtggc cgagcgcacg cgggagctgt cggatctggc
    12181 gagctacctg accaccgcgc gggaactcga acaggcgcgg ctggcccgcg agctgcacga
    12241 cgaactgggg gcgctcctca ccgcggccaa gatggacgcg agctggttgc tgcgcagcct
    12301 gggcgcgcag tccacgccgg acatccaggc ccgtttccgg cgcctgatcg acaccatcgg
    12361 ttcgggcatc accatcaagc ggcgtgtggt gagcgacctg cgtccgccgc tgctccaggg
    12421 gctgggcctg gtggaagccc tgcgagccct gtccgacgat ctccacgacg agtaccgggt
    12481 cacgctgcag ctgcccggcg agaacatcga ctgcgacgag gaacaggccc tggccctctt
    12541 ccgcatcgtc caggaaagcg tgaccaacat ccgcaagtac gcgcgggcca gggccatcga
    12601 ggtgggtctg gcggtcgacg cgggggtcgt ccggctatgg gtgaaggacg acggtgtcgg
    12661 cttcgacccc gacaacccgc agctcaatcg ccatggcctg gccgggatga agcaccgggt
    12721 gcagatgttc gaggggcgtt ttacggtgac gtctgcaccc ggcgccggaa cgcgcatcga
    12781 cgccacgatg cccctgcggc cggcggcccc gtcggcgcac ggctgaatcc gacgcaccca
    12841 cgccggcggc gggctccggc gtggcgcgga cccgcccgtg ccggcggctc agtcgacgat
    12901 cagaccgtgg cgcagggcgt aggcggcgag ttccgcattg ctggcgacgt cgagcttttc
    12961 caggaggcgg gagcgatagg tgctgaccgt cttcacgctc agattgagca cgtcggcgat
    13021 accggacacg gattcccccc tggcgaggcg caggaagacc tgcagctccc gctcggagag
    13081 gctctcgtgg ggcagtgcgg tgcccgcgcc ggccagatcg tccgccagca attcggcggt
    13141 gcgcggtgac acatagcgcc gaccctgcgc gaccgtgcgg atcgcgcgga tcagctcgtc
    13201 ccgttcgcag tccttgcaca ggtagccgtt ggcgccgttg cggatcatgg gcagcgcata
    13261 gcgctcctcg gggaagccgc tcagcaccag caccttgaga tccgcgtagc gctgccggac
    13321 ggcccgcagc acatccacgc cgctctgccc cggcaaggac agatccagca gcaggacatc
    13381 gcagggcgtc gtgcgcaggt gctgcagggc ctcctcgccg gaggcggcct cgaactccac
    13441 ctggatgccc agttcatcgg agagcatttc gcggaatccc gcacgcacga tcttgtggtc
    13501 gtcgacgatg gccagacgca gcatggcggt tctcccggac ggaatggacg gattgtatcg
    13561 gtggggcgcg cggcggcgcg tcccccgagt ccgacaatca tgcaggaatt gtcctacggg
    13621 cgggcgccga ttcccgtgcc gcaggggcgc ccgcatgtag gtgccggcct acaggccaat
    13681 cggcggtgga tgactgcgcg ccaggccggg acgggggagg atgcaagcat caccgtcgcc
    13741 cgtccatcgc tggaatgccg ccatgaagat cgtccgccgt cgtccccttg ccctggaagc
    13801 gtccgtccgg atcggcctgc tggccgtcac cttcgttgcc gcgtttgccg cgggacattc
    13861 gaccgcgtcc cgcgcgccgc agtcgctgga tgacccggcc gcggggcccg ccctgctgcg
    13921 cgacggggtc gatcgggccg gccggagcgt tgccgcggcc gtcggcctcg acgtgtattc
    13981 cgaggcgggc cggcccgtcg ggcgcctgac cgccctcctg cacggcgaag gccacgatgg
    14041 cctggcccgg atcgccgtgg ccgaacgggg cgacgaaggc gcacgggaga tcgtggtgcc
    14101 gctcggccgc ctggtctggc gggacgatcg cgtggtgctg gcggacggcg ccgcgccgcg
    14161 ctggctgatc tgaggcggcg caagtggcca ccgcggcggc gcgcccgcag gaccgcgcga
    14221 cccgcctgaa ggtccgccgg gcacgcaaga tggcccgctc ggcccacgcc tacgtgcggg
    14281 gcagcacagc ccgtttttac gcctggctgg cggcgagcga cggcgacgcc ctgccggacg
    14341 gtccgcccgt ctggatctgc ggtgactgcc acctgggcaa cctggggccg gtggccgaca
    14401 gcgatgggcg taccggtgtc gagatccgcg acctcgatca ggcggtgatc ggcaacccgg
    14461 cccatgatct cgtccgcctc gggctgtccc tggcctgcgc ctgtcgcagc gccgacctgc
    14521 ccggggtcat gagtctccgt gtcgtcgata tgctcctgga cgggtatgcc cgtgccttcg
    14581 cgcagcgtcc gcgtccaccg tcgccgccgg acagcgtgca ggcggtgatc cgtcgggccg
    14641 tgaagcgtac gtggaagcag ctgatgcgcg accacctcga ccacgcccgt ccgcggatcc
    14701 ccctcggcgc ccgcttctgg ccgctcaccc gccgcgagca gggggccatc gcacagtgtc
    14761 tggacgagac ccgcctgccc gaactggcgc gccagctggg catcgcgcgg gacaccgagg
    14821 cggtgcgcgt ggccgacgcg gcctattggg tcaagggctg cagcagcctc gggcggcccc
    14881 gctacgtcgt cctgcttcag gtggacggcg cggccgaggc gcgtctggcg atggtggaca
    14941 tcaaggatgc gccgcccgcc gcggcacccc gcaccgggtc ggtgagcatg ccccgggaca
    15001 acgccgagcg ggtggtggag gcggcccgtg ccctggcgcc ggcgctgggc gggcgcatgg
    15061 tggcgatgcg tctgctcgac cggccgatgg tggcgcgcga gctgtcgccc caggacctca
    15121 agatcgagcc ggaccagctg gccccggcag acatggcggc cctggcgcac tacctggggc
    15181 gcatcgtcgg cgatgctcac gcccgtcaga tgtcggcccc gcagcgtcgt cactggcatg
    15241 ccactctgcg ccgttcccac agccgcgtcg tcgacgcccc gtcgtggctg tggcgcgcca
    15301 tcgtggacgc cctggcggat catgagcggg gctatctgga acattgccgg cggcacgtcg
    15361 cagcgctcgt cgcgtgaggg tcgagcatcc cgggcgcagc cccgcgccct gtaggaactg
    15421 accgacagac ccgccccggc gggcgcccta tactggattc gtccggaagc gcgcaggccg
    15481 ccgccgcgcg cagccggccc gcaccctccg tcacgcgacg cttctgaacg ccatcttctg
    15541 cccacgcgcc caccatgccg tctgaaccca ccgtcctgcg aacggtcatc atcgaggaca
    15601 acgccgtgct cctcgacttg ctgacgggca tgctggcggg tatcgaaggg gtgagcgtgg
    15661 tggggcacgc ggcgagtgag gccgaggccc tggcgctggt gcgcgccacc cggccccagc
    15721 tggccatcgt ggatctggag ctgcgcggca gttctggtct gggcgtgctc gccgcctttc
    15781 agcagacgcc tgacgagtac ggcgcgccgc tggccatcgt gttctccaac cacgcccatc
    15841 ccatcgtgca gtcccgctgc ctggcgctgg gtgcgcgggc gttcttcgac aaatccttcc
    15901 agatggacga tctgctcgac ttcgtgcagg ccacggcgcg cgccctctga gggcgctccg
    15961 ttcgtccgcc tgcgccatcg ggcgccagcg gcgttcacga cgcagggggc ggaagcccgc
    16021 cccctgctgc cgctgtgcgt gcgccttacg ggcgcacgac gatgtcgttc ttcaccatcg
    16081 acacgccggg cgtgccgcgc gccagggatt cggcccgatc ccgctccgtg gtgctcttcg
    16141 cgaagccgga cagctgcacc gtccccttga gcgtttccac cccgatggcc atcgcgctca
    16201 ccgtgctgtc ctcggcgaac ttggccttga cgcgggtcgt gatggtggcg tcatccacgt
    16261 actgtccgac ggtggattgt tcgcgggtga tggcgcagcc cgccgcggaa aaggcgagca
    16321 gaccggcaag ggcaagggtg acggcggtgg ttttcatgac aggctcctca tctggggaaa
    16381 gtcggtccgg tagacatgtg ctccggtacg tactgctgac tccagcatag ggcggggcca
    16441 cgccggcgcc tgtccgacgg gacggattga cgtgtaggac tcgtccttcc cggcgccccg
    16501 gcgcctacgg ccacggtgcc gccgcgcgcg ccgccatccg gctgaaggcc gggcgcgcgc
    16561 ctgtggcgcc cagtgcggcg cggagcgtgc catgcacggg gcatttgctg cgcagcatct
    16621 aaaccattgt ctaatttctg gacaggccga ttccccctaa ggtgtctcca cacggtcgat
    16681 ccgctgggcg cggaggaccg agggggagct tgcagggctc ctgcatcccg acatgaagga
    16741 gacaagaatg gccttactca atcggatgga ctggtacgac ctggcccgat ccaccaactg
    16801 gaccccgaag tacgtcaccg aggacgaact gttcccgccc aagctgtcgg gcgagttcgg
    16861 cctgccgctc acggcctggg agtcctacga cgaaccctac aagcagacct acccggaata
    16921 cgtgaaggtg cagcgcgaga aggatgccgg cgcctactcg gtgaaggccg ccctggagcg
    16981 cagccgcatc ttcgagaacg ccgatccggg ctggaagtcg gtgatgaagg cccactacgg
    17041 cgccatcgcc cggggcgaat acgccgccgc cagcgccgag gcgcggatga tgcgtttctc
    17101 caaggcgccg ggcatgcgca acatggccac cctgggctgc atggacgaga tccgccacgg
    17161 ccagatgcag ctctacttcc cccacgagca cgtggccaag gaccggcaga tggactgggc
    17221 cttcaaggcc tacgacacca atgactgggc catgatcgcc gcccggcatt tcttcgacga
    17281 catcatgatg acccgggacg ccatcagcgt ctccatcatg ctgaccttca gcttcgagac
    17341 cggcttcacc aacatgcagt tcctggggct ggcggcggac gcggcggaag ccggcgacca
    17401 caccttcgcc aacctcatct ccagcatcca gaccgacgag tcccgccacg cccagatcgg
    17461 tggcccggcc ctcaaggtgc tcatcgagaa cggccagaag gccgaggccc agaagcgcgt
    17521 ggacatcgct gtgtggggcg cctggaagct cttctcggtg ctgaccggtc cgatcatgga
    17581 ctactacacg ccgctggagc accgcaagca gtccttcaag gagttcatgg aggagtggat
    17641 cgtcgcccag tttgagcgcg ccctcaccga catgggcctg gagctgccct ggtactggga
    17701 gatcttcctc aaggatctct ccgagaccca ccacggcatg cacctgggct cctactactg
    17761 gcgccccacc gtgtggtgga accc
//
LOCUS       DAOQPA010000075        17713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1607_length_17713_cov_17.927115, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000075 DAOQPA010000000
VERSION     DAOQPA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_17713_cov_17.927115"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKX01_11155"
     CDS             <1..660
                     /locus_tag="PKX01_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HMZ76729.1"
                     /translation="FADLLRQGAIKQDVQVLLTDSTEAEAIKLFANTYLAMRVAYFNE
                     LDSYAAAHGLDTRQIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYRDVPQN
                     LIHAIVESNTTRKDFIADEIIRRRPRTVGIFRLIMKAGSDNFRASSIQGIMKRIKAKG
                     IEVIVYEPVLKQDEFFNSRVVNDLAAFKAQSDVIIANRRTDALADVGDKVFTRDLFGS
                     D"
     gene            799..1542
                     /locus_tag="PKX01_11160"
     CDS             799..1542
                     /locus_tag="PKX01_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HMZ76730.1"
                     /translation="MKKTLLAYGAAARSLLRADILWHLMWPTILSAALWVGGLAFGWS
                     ALVASAQDGLVALPWLGSWLAGSPGALVVALVLFKIALVVLVLPVIYVTAAFLVAAIA
                     LPLMLETVARTDYADLARRGGGSTWGSVWNALVAVIVFLLLLVASLPLWLIPGAGLVI
                     SLLLTAWLNQRAFRYDALMTHADAYELKALPQQQSAGLFGVGLLGAVLAHVPLLNLFA
                     PAVTGLAFVHYLLAALRDARRTENVIEVV"
     gene            1543..2334
                     /locus_tag="PKX01_11165"
     CDS             1543..2334
                     /locus_tag="PKX01_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HMZ76731.1"
                     /translation="MSDLREAAPAFGAIIIGDELLSGRREDKHLAKVVELLKARGLRL
                     ASARYVGDERPRLVQTLRESFASGDIVFSFGGIGATPDDHTRQAAAEALGLPLALHPE
                     AEAEIRGRFGDETTPQRLQMGVYPVGSAIIPNPYNRIPGFSIRQHFFVPGFPVMAWPM
                     VEWVLDTHFAHLHHRTDYVERSILVWEALEGKLIDLMETLTADYPDLTLFSLPCFGGD
                     EVRRHVELGMKGSAARVDEAMARIVTEVARRGYRWDAPGADASAA"
     gene            2345..2722
                     /locus_tag="PKX01_11170"
     CDS             2345..2722
                     /locus_tag="PKX01_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76732.1"
                     /translation="MSGGRSGYRDFPGRRWLVVALRAGHMAGVVGVGAALLAEPMVAP
                     EPAFLVLLVAAGAAMALLDAYSNPDWLREPAGWSLFAKFALLGWYMADAAVRLPLFWA
                     ILVFSVLFAHAPASFRHRRPNGR"
     gene            2805..3356
                     /locus_tag="PKX01_11175"
     CDS             2805..3356
                     /locus_tag="PKX01_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76733.1"
                     /translation="MELSDIPVKTAAGHEEIARRDRRLPTRLRTLLILADGKRSVQAL
                     ADATVGFGPVDEPLAHLVTLGLIEPMRKAAPPDPSSAPALPASRAAQPQGVPEKPAHE
                     RPAPRPGQRRSLALARLYLLNAMEQSLRQDDQPIRDCLRAAKTRAELLQAFEVCREVA
                     QEVGVKHIRTIEAQFFEMLPEEG"
     gene            complement(3396..5447)
                     /gene="fusA"
                     /locus_tag="PKX01_11180"
     CDS             complement(3396..5447)
                     /gene="fusA"
                     /locus_tag="PKX01_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMZ76734.1"
                     /translation="MTPTSVRDIRNLSLLGQSGSGKTTLLEKLLATAGAIGAPGSVEK
                     GDTVSDFDPQEKTFGHSLNTALAHLEWAEHWVNLLDTPGLPDFLGRALASLPAVETAV
                     VVINAAAGVETVTRRIMDAAADKCRMLVVNRIDAPGVDFGALMEHIVASFGKECLPIN
                     LPLAGGTGVVDCFFKPDYDAVPAFSSVREAHDRIVDQVVEVDEDLMALYLEQGESLQP
                     AQLHDPFEQALREGHLIPVCFTSARSGAGVSELLDILGRLLPDPTEGNPPHFLKGEGP
                     AAQPVEATTAADQHVIAHVFQVSNDPFRGKLGLFRIHQGTVTPATQLFVGDARKPFKV
                     GHLLRIQGKTQTEITVGIPGDLCAVARVDEIHRDAVLHDSHDEDHFHLVPPRYPQPVF
                     GLALQPEKHGDEQKLSDALHRLLDEDPCLSVEHDTQAKETVIRGLGEVHLKVVLEQLK
                     TRWNLSLVTRPPAIPYRETIAGTAEARYRHKKQSGGAGQFGEVALRVETLERGAGVVF
                     ADEVKGGVIPGNFMPAVEKGVHQAVIEGVMAGFPLQDLRVVVFDGKHHAVDSNEISFV
                     TAARHAVQEAVQAARPVVLEPLVTLTVRIADEHFGGISAELSGRRGRVTGTDAPQAGV
                     TEITSLVPMAELEGFESRLKSISGGDGSCAIAFSHYEPAPAEVQQRLAREHAGKRGGE
                     Q"
     gene            complement(5541..7256)
                     /locus_tag="PKX01_11185"
     CDS             complement(5541..7256)
                     /locus_tag="PKX01_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ76735.1"
                     /translation="MDRFLPFLAWRRRLDAAGLRADLLAGVTVALVAIPQALAYAQLA
                     GLPPYIGLYASLLPSIVGALFGSSAQLNTGPVALTSLLTATTLAPLAPPGSQTYLLLA
                     VQLALLSGLLQVIFGALRLAHFADLLSHPVLHGFINACAVLICVAQLPALLGLPIHGQ
                     RPFVEAVVEILTRLPELHLPSAALGGAAVLMLILLRRFAPRWPGILIVVASLTLVSEA
                     IDFAAQGGRVVGTLPEHIVAFDWPGMDWSQMTELLPSAFVLALVSFLEAMSSCKVASA
                     RTGERWDGNQELIGQGLAKLTASVCQAYPVSGSFGRSAFLLAAGARTGLASVVGAVMV
                     LAALLAAPELIARIPRPVLAAVIFVTLSALLSPKPLQEAWQASRDDGLAGTVSFAATL
                     LLAPHIEIGILSGLILSLAFLIYRSMTPRVALLGRHPDGTWRDADRFRLAPPHPRLII
                     LRFDGPLHFVNAATFEDAVLDATRRHPQLQVLLLACAGINDMDASGVDTLRRIHRQFH
                     EAGHTLACCGLKKQVIDVLERTGLWAGLAPHAAYRTEDHALSALLPLVGVAAPDPHAR
                     RGLAW"
     gene            7532..8686
                     /locus_tag="PKX01_11190"
     CDS             7532..8686
                     /locus_tag="PKX01_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HMZ76736.1"
                     /translation="MNDFSDTALTPETATLLIVDDVPENLEVLAELLQSRYRVRAAQS
                     GARALQVACTPPRPDLILLDVMMPDMDGFEVAARLRADPATRDIPVIFVTALDGVQEE
                     EHGLELGAVDYISKPLRPSIVLARVNTQLELKRARDHLRDHNEFLEAEVARRMGENQL
                     IQDVSIRALARLAEIRDPEMGYHLQRTQAYVELLARSLRDHPRFADTLTPRTILLLGK
                     SAPLHDIGKVGIPDHILLKPGKLTPDEWEIMKTHAKLGSDAIELAEKDEAQPVAFLTF
                     AKEIARHHHERWDGSGYPDGLRGDEIPLSARLMALADVFDALTTRRVYKAPMSMETAR
                     RIIVEGRGNHFDPDVVDAFLAAFDGFAEIALRYRDEFPEIDALQSTTARD"
     gene            8774..13144
                     /locus_tag="PKX01_11195"
     CDS             8774..13144
                     /locus_tag="PKX01_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76737.1"
                     /translation="MVLVILAAGYGLGVMVSADRNGNESRRLEAVARLKQQQLQTWLT
                     ERRADARSLGQDRSMAELVTRVQGGDRVARQWLQDRLVSVGQAYDYATVTLVNSRGEP
                     LVGLPALPDAFAARLRALWQDGHPGEQSVWADLASGQEGLAFIAPVAGAGDVAAVLLA
                     DPSRSLFPTLQTWPSPSVSAETLLARRDGADVEFLNSVRHRQVETGTFRMPLDWTPVP
                     LVMEGMLHPERMGTVLLGVDYRGVPSAGVVLPVAGTDWVLVTKMDRDEWQADARRAVS
                     WIALGCLAAVMVVGAGALLRYQRQELTHSRRVFRAQAEQLRGLRLLDAIAGTSPDAML
                     AWDRDGVIRFCNPAASRLIGVEAESLVGSRGERARASEALQRIQALASAGGAVVEAVH
                     GSEVRVGEGAAARIHYVTVGPLPDADGEGGVFAMARDITSLRREEEALREREELYSAI
                     VNQAADGIVLLDAQSLNFVEFNDAACHGLGYSRDEFAALNLRDVQQVPVRDVSELRVT
                     RLAVGEAVSFSNLHRRKDGSVRQVELNYRSLNIRGHAFLIGVWRDTTDKLEAEERLRK
                     LSMAVEQSPTSVLIADMAGTIEYVNEAFLHSTGYAREEVIGQMAGFNRSGVTPKETIK
                     ALWDALQAGQAWEGEFINRRRDGELRIEHARIAPILRADGSVSHYLGLQEDVTRRKQV
                     EAELAQYRQHLEERVAERTAQLEEAYRDLTGRSAQVAELNAALERRAAELEAARDASD
                     AASRAKSAFLANMSHEIRTPMNAIIGMTHLLRRGVLDAEQQGRLEKVGDAAEHLLSII
                     NDVLDISKIEAGKLSLDVSDFRVEEVIRKACGLVADKAREKGLELVMDVDEPPYVLKG
                     DATRVGQMVLNYLGNAVKFSSSGVVLVTARVEDEGNSSARLHVAVRDQGVGISPEALP
                     RLFRPFEQADGSTTRKYGGTGLGLAITGHLARLMDGTTGVESTPGQGSLFRFSVRVDR
                     GDPRPAVPSGLPALSALVVEPVAEARASLARILNVLDVHVRVVDSDAAAQAALAEMAA
                     RGEHPDVVFTGPGRGGPAEAVADPRIRFVRVTAGDDARGQSIQTGGAAATLTKPLGVS
                     AVLETLQALFVHAEGAVAVSAGRSPAELELGRRHAGARILLVEDSPINQEVAVALLEG
                     CGLVVDVAEDGARAVEHMQAHAYALVLMDMQMPVMDGLEATRRIRALPGGASVPVVAM
                     TANAFGEDRARCLAAGMNDHLAKPVDPAQLYAMLLRWLGGAPATGPAATSDAERLREA
                     LEAIPGLDVDYGLKNLRGRVSSFVRLLRKYADGHADDPQRIRAARQAGEHAEAARIAH
                     SLKGVAGMIGVRTVQEAATRLDAALKAGEGEARVAELIDALAVQQAAAVAGIAALGEG
                     VAEAAAEVDPTLIRQVLGQLEALLREDNVAVGRVLREQLAVVRAGLGADAGPFERAVA
                     AFDFARALALLESAQTVQPH"
     gene            13199..14419
                     /locus_tag="PKX01_11200"
     CDS             13199..14419
                     /locus_tag="PKX01_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ76738.1"
                     /translation="MKTSDPAGPGASHWPLVLLASAVLMITMGVRQSLGLYLSPLNTA
                     TGLGVASISFAMAVGQFMWGAAQPVFGAVADRYGPARALFTGAVMLVAGLALTPFVTS
                     ETGLILCLGVLVAAGAGAGSFSILIGALAQRVPPQKRAFASGFINAGGSFGQFLFAPL
                     SQFLITGYGWVVALFGMAAAAALTIPLSWPLRHKVEHPPAELGAETGLKAQLRVALRD
                     RSYWCLHAGFFTCGFHIAFLVTHLPGAVNLCGLPASVSAVSLAVIGLANIVGSLGAGA
                     LGQHYRMKWILAVMYASRAVLVALYLMAPKTEWTFYLFAVGLGVTWLATVPPTAGLVG
                     KLFGLRYLATLFGLTLLSHQIGGFFGAWLGGLALVNFGNYDWMWYADIVLALLAALAN
                     VPIREVSPRRAVAV"
     gene            complement(14465..>17713)
                     /locus_tag="PKX01_11205"
     CDS             complement(14465..>17713)
                     /locus_tag="PKX01_11205"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HMZ76739.1"
                     /translation="YREDYDARSAVGDIELMESLAADGHLAMSLYVPLEAPPGTLRFK
                     VFRDTDPVPLHVSLPLLEHMGVRVLEERPYEIERADGQKVWMHDFGLSVRGADEIDVD
                     RVRGAFQDAFRHILAGDAESDDFNRLVLAVGLTWREVAVLRAYAKYLRQTGFTFSQAY
                     MEQTLAAHPALARQLLELFIARFDPDLGEDRAEHVASLAAALQADLDEVANLDEDRIL
                     RQFLAVIQASLRTNWFRRDGGAPRPCLSFKLDPHAIPGLPEPRPLYEIFVYAPRVEGI
                     HLRGGKVARGGLRWSDRMEDFRTEVLGLMKAQMVKNAVIVPVGSKGGFVVKNPPPASD
                     RDAFLKEGVACYHLFLRGLLDLTDNRVGGAVVPPPDVVRHDGDDPYLVVAADKGTATF
                     SDYANGVSAEYGFWLRDAFASGGSAGYDHKKMGITARGAWESGKHHFRALGVDIQTQA
                     FTVVGVGDMSGDVFGNGMLLSRQTRLVAAFDHRHIFIDPMPDPATSFAERERLFALPR
                     SSWADYDARLLSPGGAIVPRSAKSVSLSPEARTALGIEADTLTPQELVRAILVAPVDM
                     LYNGGIGTYVKASSQRHAEVGDRANDAVRVDGRDLRCKVVVEGGNLGFTQLGRIEYAR
                     AGGRINTDAIDNSAGVDCSDHEVNIKILLNLVIEDGELTEKQRNALLAGMTDEVALLV
                     LRDNYFQNSILSGIRARGTRMLDEHARYIRHLVARGRLNRDVEFLPPDDEIAARKAAC
                     QALTAPELAVLLAYGKMELFDALLASDVPEDPYIRTALHRYFPAPLRERYPQHIERHP
                     LRREIIATHVVNSMVNRVGPSFAYRLSEETGAPLPDVVRAYIATREVFGFVPLWQRIE
                     ALDNRVDHGAQIKLVYEGWRLIQRGTLWFLRHREHLRDLAETLARFAGSVDQLAAGLY
                     DLITPAYRDQLDQGMAVYLEQGVPADLVRHVACLDALYAALDIVETGAETGRPVELVA
                     RVYFALAGCLDLHRLARRIGTLPTDSHWQGLARNALRDDLSTQARGLATDVLRETPDE
                     ADPFRLLEHWKARRAFELERCRQIVAEVLAAPAADLAMLSVALRELRGLAMK"
BASE COUNT         2564 a         5585 c         6753 g         2811 t
ORIGIN      
        1 tttgccgatc tgctccgcca gggcgcgatc aagcaggatg tgcaggtgct gctgaccgac
       61 agcaccgagg cggaggccat caagctcttc gccaacacct acctggcgat gcgggtggcc
      121 tacttcaatg agctcgacag ctatgcggcg gcccacgggc tggatacccg ccagatcatc
      181 gacggggtat gcctggatcc gcgcatcggc agccactaca acaacccgag cttcggctac
      241 ggcggctact gtctgcccaa ggacaccaag cagctgctgg ccaattaccg ggatgtgccc
      301 cagaacctga tccacgccat tgtcgagtcg aacaccacgc gcaaggactt catcgccgat
      361 gagatcatcc gtcgccggcc gcgcacggtg gggatcttcc ggctgatcat gaaggccggt
      421 tcggacaatt tccgggcgtc gagcatccag ggcatcatga agcggatcaa ggcgaagggc
      481 atcgaggtca tcgtctatga gccggtgctc aagcaggacg agttcttcaa ctcccgcgtg
      541 gtgaacgatc tggcggcctt caaggcccag tcggacgtga tcatcgccaa ccggcgcacg
      601 gacgcgctgg cggatgtggg ggacaaggtc ttcacgcggg atctgttcgg cagcgactga
      661 tctcccgtgg aggcgccggg cgggaggcgg tgcctgcccg atctccggcc gcgcgacctc
      721 gatggctggt atcccatgtc cgcttcgggg acaatgccgc ccgtggcggg ccggcacggc
      781 cgcgaagcgg agcgagcaat gaagaagacc cttctggctt atggggcggc ggcgcgttcg
      841 ttgctgcggg ccgacatcct gtggcatctc atgtggccga cgatcctgtc ggcggccctg
      901 tgggtgggcg ggctggcctt tgggtggagt gcgctggtgg cgtcggccca ggacgggctg
      961 gtggcgctgc cctggctcgg cagctggctg gcaggttcgc cgggggcgct ggtggtggcg
     1021 ctggtgctgt tcaaaatcgc gctggtggtg ctggtcctgc cggtgatcta tgtgacggcg
     1081 gcctttctgg tggcggcgat cgcgttgccc ctgatgctgg agacggtggc ccgcacggat
     1141 tatgcggacc tggcgcgccg tggcggcggc agtacctggg gcagcgtgtg gaatgccctg
     1201 gtcgcggtga tcgtcttcct gctcctgctg gtggcgagcc tgcccttgtg gctgatcccc
     1261 ggcgccgggc tggtgatctc cctgctcctc accgcgtggc tcaaccagcg ggccttccgt
     1321 tacgatgcgc tgatgaccca cgcggatgcc tacgagctca aggccctgcc gcagcagcag
     1381 tccgccgggc tgttcggcgt gggcctgctg ggggcggtgc tggcccacgt gccgctgctc
     1441 aacctgtttg cgccggccgt gacggggctg gccttcgtcc attacctgct ggcggcgctg
     1501 cgggatgcgc gtcgcaccga aaacgtgatc gaggtggtgt gaatgtcgga tcttcgagaa
     1561 gccgcgccag cctttggcgc gatcatcatt ggcgacgagc tgctctccgg gcgtcgcgag
     1621 gacaagcatc tggccaaggt ggtggaactg ctcaaggccc gcgggctgcg gctggcctcg
     1681 gcgcgctacg tgggggacga gcggccgcgg ctggtgcaga ccctgcgcga gagcttcgcc
     1741 agcggcgaca tcgtgttcag tttcgggggc atcggcgcca cgccggacga tcacacccgc
     1801 caggcggcgg ccgaggcgct ggggctgccc ctggccctgc atccggaggc cgaggcggag
     1861 atccgcgggc gcttcggcga cgagaccacg ccccagcgtc tgcagatggg cgtgtatccg
     1921 gtgggcagcg cgatcattcc caatccctac aaccggattc cgggcttctc gatccgccag
     1981 cacttcttcg tgcctggctt tccggtgatg gcctggccga tggtggagtg ggtgctggat
     2041 acccacttcg cgcacctgca tcaccgcacg gactacgtgg agcgttccat cctggtgtgg
     2101 gaggcgctgg aggggaagct catcgacctg atggagacgc tgaccgccga ttacccggac
     2161 ctgaccctgt tcagcctgcc gtgctttggc ggtgacgagg tgcgccggca tgtggagctg
     2221 ggcatgaagg gttcggccgc ccgcgtggac gaggcgatgg cacgcatcgt gaccgaagtg
     2281 gcgcggcggg gttaccggtg ggacgctccg ggcgcagacg cgtcggcggc gtaagggtgc
     2341 gtcggtgagc ggcggacggt ccggctatcg ggattttccg gggcggcgct ggttggtggt
     2401 ggccctgcgg gccgggcaca tggcaggtgt ggtgggcgtt ggcgcggccc tgctggccga
     2461 acccatggtg gcgccggagc cggcctttct ggtgttgctg gtggcggccg gggcggccat
     2521 ggcgctgctg gacgcctatt ccaatccgga ctggctgcgg gagccggccg gctggtcgct
     2581 gttcgccaag tttgcgctgt tgggctggta catggccgat gcggcagtgc gtttgcccct
     2641 gttctgggcc atcctggtgt tctccgtgct gttcgcccac gcgccggcga gcttccggca
     2701 ccgtcgcccc aacgggcgct gagcggggcg cggcgtgacg gatgtcacgc tgctgcagtc
     2761 tgcgctgcgt atcctcccgg acgctttaaa gtggaggatg cgcgatggag ttgtcggata
     2821 ttcccgtcaa gacggcggcg ggacacgagg agatcgcgcg tcgcgaccga cgtctgccga
     2881 cccgcctgcg gaccctgctg atcctcgccg atggcaagcg gtcggtgcag gcgctggcgg
     2941 acgccaccgt cggcttcggg ccggtggatg agcccctggc ccaccttgtg acgctcggcc
     3001 tgatcgagcc gatgcgcaag gcggcacctc cggatccatc gtcggccccc gcgctgccgg
     3061 ccagtcgcgc tgcgcaaccc cagggcgtgc ccgagaagcc ggcgcacgag cgcccggcgc
     3121 cgcgtcccgg ccagcgccgt tccctggcgc tggcgcggct ctacctgctc aatgccatgg
     3181 agcagagcct gcgtcaggac gaccagccga tccgcgactg cctgcgcgcc gccaagaccc
     3241 gcgcggagct gttgcaggcc tttgaggtgt gccgggaggt ggcccaggag gtgggcgtca
     3301 agcacatccg gaccatcgag gcccagttct tcgagatgct gcctgaggag ggctgagacg
     3361 ggggccgggc cgaggccccc gtccgcacgg gcggcttact gctcgccgcc gcgcttgccg
     3421 gcgtgttccc gggcgaggcg ctgctgcacc tcggcagggg ccggctcgta gtggctgaag
     3481 gcgatggcgc agctgccgtc gccgccgctg atggacttga ggcggctctc gaagccttcc
     3541 agctctgcca tgggcaccag gctggtgatc tcggtgacgc cggcctgggg cgcatcggtg
     3601 ccggtgacgc gcccgcgccg gccggagagt tcggcgctga tgccgccgaa gtgctcgtcg
     3661 gcgatgcgca cggtgagggt cacgaggggt tccagcacca ccgggcgggc ggcctggacg
     3721 gcttcctgca cggcgtgacg ggcggcggtg acgaaggaga tctcgttgga atcgacggcg
     3781 tggtgcttgc cgtcgaagac caccacgcgc agatcctgca gcgggaagcc ggccatcacg
     3841 ccttcgatca cggcctggtg cacgcccttc tcgacggcgg gcatgaagtt gccggggatc
     3901 acgccgccct tcacttcatc ggcgaagacg acgccggcgc cgcgctccag ggtttcgaca
     3961 cgcagggcca cttcaccgaa ctgaccggcg ccgccgctct gcttcttgtg acggtagcgg
     4021 gcttcggccg tgccggcgat ggtctcgcgg tagggaatgg cgggcgggcg ggtgaccagg
     4081 ctgaggttcc agcgggtctt gagctgttcc agcaccacct tgaggtgtac ctcaccgagg
     4141 ccgcggatga cggtctcctt ggcctgagtg tcgtgttcga cggagaggca ggggtcttcg
     4201 tcgagcaggc ggtggagggc gtcggagagc ttctgctcgt cgccgtgctt ttccggttgc
     4261 agggcgaggc cgaagacggg ctgcgggtag cgcggcggga ccaggtggaa gtggtcttcg
     4321 tcgtgggagt cgtggagcac cgcgtcccgg tggatctcgt ccacccgcgc cacggcgcac
     4381 aggtcgccgg ggatgccgac cgtgatctcg gtctgggtct tgccctggat gcgcagcagg
     4441 tggccgacct tgaagggctt gcgggcgtcg ccgacgaaga gctgggtggc cggcgtgacg
     4501 gtgccctggt ggatgcggaa caggcccagc ttgccgcgga aggggtcgtt gctgacctgg
     4561 aagacgtggg cgatgacgtg ctggtcggcg gcggtggtgg cttcgacggg ctgggcggcg
     4621 gggccttcgc ccttgaggaa gtggggcgga ttgccttcgg tggggtcggg cagcaggcgg
     4681 ccgaggatgt cgaggagttc gctgacgccg gcgccgctgc gggcagaggt gaagcacacg
     4741 gggatcaggt ggccttcccg cagggcctgc tcgaagggat cgtggagctg ggcgggttgg
     4801 agggattcgc cttgttccag gtagagggcc atgaggtctt cgtccacctc gaccacctgg
     4861 tccacgatgc ggtcgtgggc ttcgcgcacg gaggagaagg ccggcacggc gtcgtagtcg
     4921 ggcttgaaga agcaatccac cacgcccgtg ccgcccgcca ggggcaggtt gatgggcagg
     4981 cattccttgc cgaagctggc gacgatgtgc tccatcaggg caccgaaatc cacgcccggc
     5041 gcgtcgatgc ggttgaccac cagcatgcgg cacttgtcgg cggcggcgtc catgatgcgg
     5101 cgggtgacgg tctcgacacc ggccgcggcg ttgatgacga cgaccgcggt ctcaaccgcc
     5161 ggcagggagg ccagggcgcg cccgaggaag tccggcaggc ccggcgtgtc gaggaggttg
     5221 acccagtgct cggcccattc caggtgggcc agggcggtgt tgagggagtg cccgaatgtc
     5281 ttttcctgcg ggtcgaaatc gctgaccgtg tcgccctttt cgaccgagcc gggggcgcca
     5341 atggcaccgg cggtggcgag gagcttttcc agcagggtgg tcttgccgct gccggactgg
     5401 ccgaggaggc tgaggttgcg gatgtcgcgg accgaagtgg gtgtcatggg gcgctccctt
     5461 gtggtgtgtt ctgaagtgtc ggctgcatga cagtatcggc ccgtcaccgg cctgcaacaa
     5521 cgatctggat caaatgccct tcaccatgca aggccgcgcc gggcgtgggg atcgggcgcg
     5581 gcgacgccga ccaggggcag cagcgcggag agggcgtggt cttcggtgcg ataggcggcg
     5641 tggggcgcca gcccggccca cagaccggtg cgttcgagca cgtcgatgac ctgcttcttg
     5701 agcccgcagc aggccagggt gtgacccgcc tcgtggaact ggcggtggat gcggcgcagg
     5761 gtgtctacgc cgctggcgtc catgtcgttg atgccggcgc aggcgaggag cagcacctgc
     5821 agctgggggt ggcggcgggt ggcgtcgagg acggcatcct cgaaggtggc ggcgttgacg
     5881 aagtgcaggg ggccgtcgaa acgcaggatg atcaggcgcg ggtggggcgg tgccaggcgg
     5941 aagcggtcgg cgtcgcgcca ggtgccgtcc gggtggcggc cgaggagggc gacccgcggg
     6001 gtcatgctgc ggtagatcag gaaggcgagg gacaggatca ggccggacag gatgccgatc
     6061 tcgatgtggg gcgccagcag cagggtggcg gcgaagctga cggtaccggc gaggccgtcg
     6121 tcacggctgg cctgccaggc ttcctgcagg ggtttgggcg agagcagggc ggaaagcgtg
     6181 acgaagatga ccgccgccag caccggacgc ggaatgcggg cgatgagctc gggtgcggcg
     6241 aggagggccg ccagcaccat caccgcgccg accacggagg ccagcccggt gcgggccccc
     6301 gccgccagca ggaaggcgga gcggccaaag gagccgctca ccgggtaggc ctggcagacg
     6361 gaggcggtga gtttggcgag gccctggccg atgagctcct ggttgccgtc ccagcgttca
     6421 ccggtgcggg ccgaggcgac cttgcagctg gacatggcct ccaggaagct caccagggcc
     6481 agcacgaagg cggagggcag cagctcggtc atctggctcc agtccatgcc gggccagtcg
     6541 aaggcgacga tgtgctcggg cagcgtgccg accacgcgcc caccctgggc ggcgaagtcg
     6601 atggcttcgg agacgagggt gaggctcgcc acgacgatca ggatgcccgg ccagcggggc
     6661 gcgaagcgcc gcagcaggat cagcatcagc accgccgcgc cgcccagggc ggcggagggc
     6721 aggtgcagct cgggcaggcg ggtgaggatc tcgacgacgg cctcgacgaa ggggcgctgg
     6781 ccgtggatgg gcaggcccag cagcgccggc agctgggcca cgcagatgag caccgcgcag
     6841 gcgttgatga agccgtgcag caccggatgg gacagcaggt cggcaaagtg ggccaggcgc
     6901 agggcgccga agatcacctg gagcaggccc gacaggaggg ccagctgcac cgccagcagc
     6961 aggtaggtct gggagccggg cggggccagc ggcgccaggg tggtggcggt gagcaggctg
     7021 gtgagggcca ccggtccggt gttgagctgg gcggacgagc cgaaaagcgc accgacgatg
     7081 ctcggcagca gggaggcgta gagcccgatg tagggcggca ggccggcgag ctgggcgtag
     7141 gcgagggcct ggggaatcgc caccagggcg accgtcacgc cggccagcag gtcggcccgc
     7201 aggccggcgg cgtcgaggcg ccggcgccag gcgaggaagg gcaggaagcg gtccatgcgg
     7261 acgtctcggg cgcggggacg gattctgggc agaacgaagc gcgcattgtg tcacggcggc
     7321 gcgacgaccg ggctaaagtc gctcgcagga tgaaccgtaa tgcagcagac ggaaagccgg
     7381 tcggggatgc tccggatcga ctatcgtgaa attgattcct gccgccgggg gccggtccgg
     7441 cggcgtgacc tgggtcggtt gggggcgggc atcgccactc ctccggcggc ttccggcctc
     7501 ctctcagtct tatggcgggg accccggctt catgaacgat ttttccgata ccgccctgac
     7561 gcccgaaacc gcgacgctgc tgatcgtcga cgatgttccg gagaacctgg aagtcctcgc
     7621 ggagctgctg caatcccgct atcgggtgcg tgccgcccag tcgggcgcgc gcgcgctgca
     7681 ggtggcgtgc acgccaccgc ggccggacct gatcctgctc gacgtgatga tgccggacat
     7741 ggatggcttc gaggtcgcag cgcggctgcg cgccgatccg gccacccgcg atattccggt
     7801 gatcttcgtg accgccctcg atggcgtgca ggaggaggag cacgggctcg agctgggggc
     7861 cgtggactac atcagcaagc ccctgcgccc gtccatcgtg ctggcgcggg tcaacaccca
     7921 gctcgaactc aaacgcgccc gcgaccatct acgcgaccac aacgaattcc tggaggccga
     7981 ggtggcccgg cggatgggcg agaaccagct catccaggat gtgagcatcc gcgccctggc
     8041 ccgcctggcg gagatccgcg atccggagat gggctaccac ctgcagcgta cccaggcgta
     8101 cgtggagttg ctggcccgca gcctgcgcga ccatccgcgc tttgccgaca cgctgacgcc
     8161 ccgcaccatc ctgctgctgg gcaagtcggc gccgctgcac gacatcggca aggtggggat
     8221 tccggaccac atcctgctca agccgggcaa gctcaccccg gacgagtggg aaatcatgaa
     8281 gacccacgcc aagcttggca gcgatgccat cgaactggcc gagaaggacg aagcccagcc
     8341 ggtggccttc ctcaccttcg ccaaggagat cgcccgccat caccacgagc gctgggacgg
     8401 cagcggttat ccggacggcc tccgcggcga cgagattccc ctgtcggcac gtctgatggc
     8461 cctggcggac gtgttcgacg cgctcaccac gcgacgggtg tacaaggcgc ccatgagcat
     8521 ggagacggcc cggcggatca tcgtcgaagg gcgcggcaat catttcgatc cggacgtggt
     8581 ggacgccttc ctggcggcct tcgacggatt tgcggagatt gcgctgcgct accgggacga
     8641 gtttccagag atcgacgccc tgcaatcaac caccgcccgg gactgagtgc tcatgtcttt
     8701 gccggacacg agggcggcgg ggcggagcga gcggcaggcc cgagtccggg ccctcacccg
     8761 gaccttcgtg gtcgtggtcc tggtcatctt ggcggccgga tacggtctcg gcgtgatggt
     8821 cagcgcggac cgcaacggga acgagagccg ccgtctcgaa gccgtggcgc gcctgaagca
     8881 acagcagctg cagacctggc tgacggagcg ccgtgcggat gcccgttcgc tgggccagga
     8941 tcgcagcatg gccgagctgg tgacgcgcgt gcagggcggg gacagggtgg cgcgtcagtg
     9001 gttgcaggac cgcctcgtca gtgtggggca ggcctacgac tacgcaacgg tgacgctggt
     9061 caacagtcgc ggtgaacccc tggtcggctt gcctgccctg cccgatgcgt ttgcggcccg
     9121 tctgcgggcc ctgtggcagg acgggcatcc gggcgagcag tcggtgtggg ctgacctggc
     9181 gtccgggcag gagggcctgg ccttcatcgc gccggtcgcc ggtgcgggcg atgtggcggc
     9241 ggtactgctg gcggatccgt cccgcagtct gttcccgaca cttcagacct ggccttcgcc
     9301 cagcgtgagc gcggagaccc tgctggcgcg acgggatggc gcggatgtgg agttcctgaa
     9361 ctccgtgcgc catcgccagg tggagaccgg tacgttccgg atgccactgg actggacgcc
     9421 ggttccgctg gtgatggagg gcatgctcca cccggagcgg atgggcacgg tgctgctcgg
     9481 cgtggattac cgcggcgtgc cctcggccgg cgtggtgctg ccggtggccg gaacagactg
     9541 ggtcctggtc accaagatgg accgggacga atggcaggcg gacgcgcgcc gggcggtgtc
     9601 gtggatcgcc ctgggctgtc tggcggccgt gatggtcgtc ggggcggggg cgctgctgcg
     9661 ctaccagcgc caggaactga cccattcccg gcgcgtcttt cgggcccagg ccgaacagct
     9721 gcgcggtctg cgcctgctgg acgccatcgc cgggacctct cccgacgcca tgctggcctg
     9781 ggatcgggac ggcgtgatcc gcttctgcaa tccggcggcg agccgtctga tcggggtgga
     9841 ggcggaatcc ctggtgggca gtcgcggcga acgggcccgc gcgtcggagg ccctgcagcg
     9901 catccaggcg ctggcgagcg cgggcggggc ggtcgtcgag gcggttcacg gttcggaggt
     9961 ccgggtcggg gaaggggctg cggcccgcat ccattacgtc acggtgggcc cgctgcccga
    10021 cgcggacggc gaaggcggtg tcttcgccat ggcgcgggac atcaccagcc tgcgccgcga
    10081 ggaagaagcg ctgcgggagc gggaagagct ctacagcgcc atcgtgaatc aggctgcgga
    10141 cggcatcgtg ctgctggacg cgcagagcct gaatttcgtg gaattcaacg acgccgcctg
    10201 ccacggactc ggttacagcc gcgacgaatt cgccgccctg aacctgcgcg acgtgcagca
    10261 ggtgccggtg cgggatgtgt cggagctgcg cgtgacccgg ctggcggtgg gcgaggcggt
    10321 gagcttttcc aacctgcacc ggcgcaagga cggcagcgtg cgtcaggtgg agctcaatta
    10381 ccgctcgctg aacatccgcg gtcatgcctt cctgatcggc gtgtggcgcg acaccaccga
    10441 caagctggag gccgaagagc gtctgcgcaa gctgtccatg gcggtggagc agagccctac
    10501 cagcgttttg atcgccgaca tggcgggcac catcgagtac gtgaacgagg ccttcctgca
    10561 cagcacgggg tatgcccggg aagaggtcat cgggcagatg gcgggtttca accgatcagg
    10621 cgtgacaccc aaggagacca tcaaggcctt gtgggacgct cttcaggcgg ggcaggcctg
    10681 ggagggggaa ttcatcaacc gccggcgcga cggcgaactg cgcatcgagc atgcccgcat
    10741 cgcacccatc ctgcgggcgg acggcagcgt cagccactat ctgggcctgc aggaggatgt
    10801 cacccggcgc aagcaggtgg aggccgagct ggcccagtat cgccagcatc tggaggagcg
    10861 cgtcgccgaa cgcaccgccc agctcgagga ggcgtaccgg gacctgaccg gccgctcggc
    10921 ccaggtggcg gagctgaatg ccgccctgga gcgccgggct gccgaactgg aggccgcgcg
    10981 ggatgcgtcg gacgcggcca gccgcgcgaa gagcgccttc ctggccaata tgagccacga
    11041 gatccgtacg cccatgaacg ccatcatcgg gatgacccac ctgctgcggc gcggggtgct
    11101 cgacgcggag cagcagggac gcctggaaaa ggtgggcgat gcggcggagc acctcctctc
    11161 catcatcaac gacgtcctcg acatctcgaa gatcgaggcc ggcaagctgt ccctggacgt
    11221 gtccgatttt cgtgtcgagg aggtgatccg caaggcctgc ggactggtgg ccgacaaggc
    11281 ccgggagaag ggcctggagc tggtgatgga cgtggatgaa ccgccctatg tgctgaaggg
    11341 cgacgccacc cgggtcgggc agatggtgct caattacctg ggcaacgcgg tgaagttctc
    11401 gtcgtccggt gtggtcctgg tgacggcgcg ggtggaggac gaaggcaatt cctcggcgcg
    11461 gctccacgtg gcggtgcgcg atcagggcgt cggcatttca ccggaggccc tgccgcgcct
    11521 cttccggccc ttcgagcagg cggatggctc caccacccgg aaatacggcg gcaccggcct
    11581 cggtctggcc atcaccggtc atctggcgcg tctcatggac ggcaccacag gggtcgagag
    11641 cacgccgggc cagggcagcc tcttccggtt ctcggtgcgc gtcgatcggg gcgatccccg
    11701 gcctgccgtg ccatcgggtc tgccggccct ctccgccctg gtggtggagc cggtggccga
    11761 ggcgcgggca tctctggcgc gcatcctgaa cgtgctggat gtgcacgtcc gcgtggtgga
    11821 ttcggatgcc gccgcgcagg cggcgctggc agaaatggcg gcccgcggcg agcatcccga
    11881 cgtggtcttt accggtccgg ggcgcggtgg gcccgccgag gccgtggccg acccccgcat
    11941 ccggtttgtg cgtgtcacgg cgggcgacga tgcccggggg caatcgatcc agaccggcgg
    12001 tgcggcggcg actttgacca agccgctggg ggtgtcggcg gtgctggaga ccttgcaggc
    12061 ccttttcgtt cacgccgaag gggccgtggc ggtcagcgcc gggcgcagtc cggccgagct
    12121 ggagctgggc cggcggcacg ccggcgcccg catcctgctg gtggaggaca gcccgatcaa
    12181 ccaggaagtg gcggtggcct tgctggaggg ctgcggtctg gttgtcgacg tcgccgaaga
    12241 cggggcccgt gcggtggagc acatgcaggc gcacgcctac gccctggtcc tgatggacat
    12301 gcagatgccg gtgatggacg gtctggaggc gacccgccgc atccgtgccc tgcccggcgg
    12361 ggcttcggtg ccggtcgtgg ccatgaccgc caacgccttc ggcgaggacc gggcgcgctg
    12421 cctggcggcg ggcatgaacg atcatctggc caagccggtg gacccggccc aactctacgc
    12481 catgctgctg cgctggctgg gcggcgcgcc ggcgacgggg cccgcggcga cttccgacgc
    12541 ggagcggctg cgggaagcgc tggaagcgat tcccgggctg gacgtggatt acggactcaa
    12601 gaacctgcgc ggccgggtga gcagcttcgt gcgtctgcta cgcaaatacg ccgatggtca
    12661 tgcggacgac ccgcagcgca ttcgcgcggc ccggcaggcc ggcgagcacg cggaggcggc
    12721 gcgcattgcc cactcactga aaggcgtggc cggcatgatc ggggtgcgaa ccgtgcagga
    12781 ggctgcgacc cgtctggatg cggccctgaa ggccggggag ggcgaggcac gggtggccga
    12841 actgatcgac gcgctggcgg tgcagcaggc ggccgcggtg gcggggatcg cggccctcgg
    12901 cgagggggtg gcggaagccg ccgccgaggt cgatccgacg ctgatccggc aggtgctggg
    12961 acaactggag gccctgttgc gggaggacaa cgtggcagtc ggccgcgtgc tgcgcgaaca
    13021 gctggcggtc gtgcgggccg ggctgggggc cgatgccggg ccgttcgagc gggccgtggc
    13081 ggccttcgac ttcgcccgtg ccctggcgct gctcgagtcg gcccagaccg ttcagccgca
    13141 ttgaccgggg cgacgttccg ggcgatgctc gggctcccca accgaaccgg tcttcataat
    13201 gaaaacatcc gaccctgccg gcccgggcgc cagtcactgg cccctggtgc tgctggcctc
    13261 cgcggtgctg atgatcacca tgggcgtacg ccagtccctc ggcctgtatc tttcacccct
    13321 caacacggcc accgggctgg gcgtcgccag catcagcttc gccatggccg tcgggcaatt
    13381 catgtggggc gcggcccagc cggtgttcgg cgcggtggcg gatcgttatg gtccggcgcg
    13441 ggccctgttc acgggcgccg tcatgctggt ggccgggctg gcgctgacgc ctttcgtgac
    13501 gagcgaaacc ggcctgatcc tgtgtctcgg cgtgctcgtg gcggccggcg cgggggcggg
    13561 cagcttctcc atcctgatcg gtgcactggc ccagcgggtg ccgccccaga agcgcgcctt
    13621 cgcgtcgggc ttcatcaacg ccggcggttc cttcgggcag ttcctgttcg cgccgctttc
    13681 ccagtttctg attaccggct acggctgggt ggtggccctg ttcggcatgg cggcagcggc
    13741 ggccctgacg attcccctgt cgtggccgct gcgccacaag gtggagcatc cgcccgccga
    13801 gttgggcgcc gagacgggcc tcaaggcgca gctgcgggtg gccctgcggg accgcagcta
    13861 ctggtgcctg cacgcgggct tcttcacctg cggcttccac atcgccttcc tggtgacgca
    13921 cctgcccggc gcggtgaatc tgtgcggtct gccggcgagc gtgtcggcgg tgtcgctggc
    13981 ggtgatcggg ctggccaaca tcgtgggcag cctgggggcc ggcgcgctgg gccagcacta
    14041 ccggatgaaa tggatcctgg cggtgatgta cgcgtcccgc gcggtgctgg tggccctcta
    14101 tctgatggcg cccaagacgg agtggacctt ctaccttttt gcggtggggc tgggcgtcac
    14161 ctggctggcg acggtgccgc ccacggccgg gctggtgggc aagctgttcg gtctgcgtta
    14221 tctggcgacg ctgttcggcc tgaccctgct gtcccaccag atcggtggct tcttcggcgc
    14281 ctggctgggc gggctggcgc tggtgaattt cggcaactac gactggatgt ggtacgcgga
    14341 catcgtgctg gccttgctgg ccgcgctggc caacgtgccg atccgtgagg tgtcgccgcg
    14401 ccgcgcggtg gcggtctgag gcgccttcgc cggggatgcc ccggcgaagg ctgaacccgg
    14461 cggcctactt catcgccagg ccgcgcagct cccgcagggc cacggacagc atcgccaggt
    14521 cggccgcggg ggcggccagg acttcggcga cgatctgtcg gcagcgctcc agttcgaagg
    14581 cgcggcgcgc cttccagtgt tccagcaggc ggaacggatc ggcttcgtcc ggcgtctcgc
    14641 gcaggacatc ggtggcgagg ccgcgggcct gggtggacag gtcgtcccgc agggcgttgc
    14701 gggcgagacc ctgccagtgg ctgtcggtgg gcagggtgcc gatgcgccgg gcgaggcggt
    14761 gcaggtccag acagccggcg agggcgaagt acacccgggc cacaagttcc accggccgcc
    14821 cggtctcggc gccggtctcg acgatgtcca gggccgcgta gagggcgtcc aggcaggcca
    14881 cgtggcggac cagatcggcc ggtacgccct gctccaggta caccgccatg ccctggtcga
    14941 gctgatcccg gtaggcgggt gtgatcaggt cgtagaggcc ggcggccagc tgatccacgc
    15001 tgccggcgaa gcgggcgagg gtctcggcca ggtcgcgcag gtgttcccgg tggcgcagga
    15061 accacagggt gccgcgctgg atcaggcgcc agccttcata caccagcttg atctgggcac
    15121 cgtgatcgac ccggttgtcg agggcctcga tgcgttgcca caggggcacg aagccgaaga
    15181 cctcgcgggt ggcgatgtag gcgcgcacca cgtcgggcag cggcgcgccg gtctcttcgc
    15241 tgaggcggta ggcgaagctc ggcccgaccc ggttgaccat gctgttcacc acatgggtgg
    15301 cgatgatctc ccggcgcagg gggtggcgct cgatgtgctg cgggtagcgt tcgcgcaggg
    15361 gcgccgggaa gtagcggtgc agggccgtgc ggatgtaggg gtcttccggc acatcggagg
    15421 cgagcagggc gtcgaacagc tccatcttgc cgtaggccag cagcacggcc agctccggcg
    15481 cggtgagggc ctggcaggcg gccttgcggg cggcgatctc gtcgtccggc ggaaggaatt
    15541 ccacgtcccg attgaggcgg ccgcgagcca ccaggtggcg gatgtagcgg gcgtgctcgt
    15601 cgagcatgcg cgtgccgcgg gcgcggatgc cggacaggat gctgttctgg aagtagttgt
    15661 cgcgcaggac cagcagggcg acctcgtcgg tcatcccggc gagcagggcg ttgcgctgct
    15721 tttcggtgag ttcgccgtcc tcgatgacca ggttgaggag gatcttgatg ttcacctcgt
    15781 ggtcggagca gtccaccccg gcggaattgt cgatggcgtc ggtgttgatg cgcccgccgg
    15841 cacgggcgta ctcgatgcgg ccgagctggg tgaagcccag gttgccgcct tccaccacca
    15901 ccttgcagcg caggtcgcgc ccgtccacgc ggaccgcgtc gttggcgcgg tcgcccacct
    15961 cggcgtggcg ctggctgctg gccttgacat aggtgccgat gccgccgttg tagagcatgt
    16021 ccacgggggc gacgaggatg gcgcggacca gttcctgggg tgtcagggtg tcggcttcga
    16081 tgcccagcgc ggtgcgggct tccggcgaca ggctgacgga tttggcgctg cgcggaacga
    16141 tggcgccgcc cggcgagagc aggcgggcgt cgtagtcggc ccacgaggag cggggcaggg
    16201 cgaacaggcg ttcccgctcg gcaaagctgg tggccggatc gggcatgggg tcgatgaaga
    16261 tgtggcggtg gtcgaaggcc gccaccaggc gcgtctggcg cgacaggagc atgccattgc
    16321 cgaagacgtc gccggacatg tcgccaacgc cgaccacggt gaaggcctgg gtctggatgt
    16381 ccacgcccag ggcgcggaag tggtgcttgc cggattccca tgcgccgcgg gcagtgatgc
    16441 ccatcttctt gtggtcgtag ccggcggaac cgcccgaggc gaaggcgtcc cgcagccaga
    16501 agccgtattc ggcggagaca ccgttggcgt agtcggagaa ggtggcggtg cccttgtcgg
    16561 cggcaaccac caggtagggg tcgtcgccgt cgtggcggac cacgtccggc gggggcacca
    16621 cggcgccgcc gacccggttg tcggtgagat ccagcaggcc gcgcaggaag aggtggtaac
    16681 aggccacgcc ctccttcagg aaggcgtccc ggtcgctggc cggcggcgga ttcttgacca
    16741 cgaagccgcc cttggagccg accggcacga tcaccgcgtt cttgaccatc tgggccttca
    16801 tcagccccag cacctcggtg cggaagtcct ccatgcggtc ggaccagcgc agcccgccac
    16861 gggcgacctt gccgccgcgc aggtggatgc cttcgacgcg cggcgcatac acgaagatct
    16921 cgtacagggg gcgcggttcg ggcaggccgg ggatggcgtg ggggtcgagc ttgaaggaca
    16981 ggcaggggcg cggtgcgccg ccgtcgcggc ggaaccagtt ggtgcgcagg ctggcctgga
    17041 tgacggcaag gaactggcgc aggatgcggt cctcgtccag gttggccact tcatccagat
    17101 cggcctgcag ggcggcggcc agacttgcga catgttcggc gcggtcctcg cccaggtccg
    17161 ggtcgaagcg ggcgatgaac agctccagta actgccgggc gagggccggg tgggcggcga
    17221 gggtctgttc catgtaggcc tggctgaagg tgaagccggt ctggcgcagg tatttggcgt
    17281 aggcgcgcag caccgccacc tcgcgccagg tgaggcccac ggccagcacc aggcggttga
    17341 agtcgtcgct ttctgcgtcg cctgccagaa tgtggcggaa ggcgtcctgg aaggcgccgc
    17401 gcacccggtc cacgtcgatc tcgtcggcgc cgcggacgga caggccgaag tcgtgcatcc
    17461 acactttctg gccgtcggcc cgctcgatct catagggacg ctcctccagc acgcgcacgc
    17521 ccatgtgctc cagcaggggc agggagacat gcaggggcac cgggtcggtg tcgcggaaga
    17581 ccttgaagcg cagggtgccg gggggcgcct ccaggggcac gtagaggctc atggccaggt
    17641 ggccgtcggc ggcgagggat tccatcagct cgatgtcgcc caccgcgctg cgggcgtcgt
    17701 agtcttcccg gta
//
LOCUS       DAOQPA010000076        17600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1622_length_17600_cov_19.436136, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000076 DAOQPA010000000
VERSION     DAOQPA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17600
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_17600_cov_19.436136"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKX01_11210"
     CDS             complement(<1..830)
                     /locus_tag="PKX01_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HMZ76740.1"
                     /translation="MSVSKKMLSLLLSAALGIVILAALGLTQMSRVYEITNYANVNVV
                     PSIQTLDKAFSPMAASRTQLWQHIAASDPAVMSTLEERISANRKKVDEALKAYEPLIS
                     DDKDKSLLAADRAALADYDQLRERVYVLSRVNKMEEARDLVMANQAIMARLWDAFEEH
                     REYNAELGRKAADEAAQTLGIARWQAITISLVTLVAVCLIGFFITRSLMRQLGGEPAY
                     AAEVVAKIAEGDLSTEVLAKAGDTSSLLYSVKCMSERLAAIIAEVRTTADSLASSSEE
                     V"
     gene            complement(861..3146)
                     /locus_tag="PKX01_11215"
     CDS             complement(861..3146)
                     /locus_tag="PKX01_11215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMZ76741.1"
                     /translation="MSIQLDDALQTFIVESRELLQQMEEALLEMEHGTDDPDTLNAIF
                     RAAHTIKGSAGLFGLDDIVAFTHNVESLLDRIRDHRLALTRSIGGLLLESSDHMGALV
                     SCAAAGEAPDLGLRARSEVLGQRLKEVMNDGAATPASADATAPAGSAVAEVDADLAAL
                     DWPDGQDLPAVATSVRSDGVSSGLWHISLRAGPDLLRNGMDPLSILHYLSTLGQIVSL
                     EMVLGAVPPLTMIDPESCYLGFELSFASAAGRDSIDGAFDFVREDSIVRVIEPYGSLD
                     DYRALLGALPEDRQLLGEMLVQCGSLTRDELAQALATRDRQAADSAVAGDPAPRLGEV
                     LVQQQLVDAPLVEAAVERQQQLRGQGTAKGGEASLIRVDAAKLDQLINLIGELIIAGA
                     GTQLIARRLKVPELAEASELLSRLVESVRDSALDLRMVQIGATFNRFRRVVRDVAAEL
                     GKDIELDIRGGDTELDKTVVERIGDPLTHIVRNALDHGIEPAAQRVAAGKSAQGRLSL
                     NAYHDSGSIVIEVSDDGGGLRKERILRKAIERGLVAEGQTLSDKEIFHLVFEPGFSTA
                     EQVSNLSGRGVGMDVVKRNIQALRGTVDLESSEGQGTTVRIRLPLTLAIIDGFLIGAG
                     PSSYVVPLDQVVECIELPAGESPEHGASYLNLRGEVLPFIRLRDLFGTGGEAPRRENV
                     VVVQYAGERAGLVVDQLYGEFQTVIKPLGKVFSHIRCIGGSTILGSGEVALIIDVPGL
                     VRQVSTRHSAAKNAPLSMLPA"
     gene            complement(3163..3477)
                     /locus_tag="PKX01_11220"
     CDS             complement(3163..3477)
                     /locus_tag="PKX01_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ76742.1"
                     /translation="MNASEQTILGTAHVVIAGEMTIYTARDWRDRLLIAMEAPGDVVL
                     DLSGVSDIDAAGIQLLASLALEARAAERALRFSAVSLRILEAFEFCRLMDFFKDALAG
                     AR"
     gene            complement(3505..3873)
                     /locus_tag="PKX01_11225"
     CDS             complement(3505..3873)
                     /locus_tag="PKX01_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76743.1"
                     /translation="MAKTILIVDDSASLRQVVSIALRGAGYDVVEGCDGRDGLTKLDG
                     RKIHLIISDVNMPNMDGITFLKALKQVPAYRFTPVIMLTTEAGDDKKREGQAAGAKAW
                     VVKPFQPQQMLTAVAKLILP"
     gene            complement(3883..5052)
                     /locus_tag="PKX01_11230"
     CDS             complement(3883..5052)
                     /locus_tag="PKX01_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76744.1"
                     /translation="MKQAGAQGPVGAMPILWYGVTVLVAGVPTLAAGGRLEVLGASLA
                     VALGVLAMAWRTARPLLPTPEPAYGPAADAPPAAVEGLDRLCEDVLPLWRDQVSMVRS
                     QTEEAITALSVRFGDLSRRVESAAGSASDDGSGNLATILNASEAELSGIVAELRRALE
                     NKDVLLGQVEELARLTSSLQSMARDVGDVAKQTNLLALNAAIEAARAGEAGRGFAVVA
                     DEVRKLSTASGETGKKIGDIIATVTSAMGDALSVSRAYAQSDAQLVSDAGRRIGEVVE
                     RLRGAAGGVVARAREVSAESHAVATEIGDVLVALQFQDRVSQVLGSVSGDMDRLQQRV
                     RSCRAELAAGLHPDPLDPIAWRRDMARQFTTPEQHALQRGANPVTAREDAGITFF"
     gene            complement(5070..5687)
                     /locus_tag="PKX01_11235"
     CDS             complement(5070..5687)
                     /locus_tag="PKX01_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HMZ76745.1"
                     /translation="MKTDDPTSLTCSTRDAARRLGISVRTAQLWVEEGRLRAWKTPGG
                     HRRILRESVERLVEEQRLAAFGPGGRFRILVLREDEAGREALGQQLEGMLPDCVVVTA
                     GNGFDALLRIGDEAPEMLISDLGLTGIDFFRMVNTLVEGARTRGMLVIVLVASEADCM
                     GVRARLPDDVVLMRTPVDEAELLALVKAFRGQWRRHRSGATEQGV"
     gene            6304..9180
                     /locus_tag="PKX01_11240"
     CDS             6304..9180
                     /locus_tag="PKX01_11240"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMZ76746.1"
                     /translation="MSAIAKDADARTAATLAPQEISGEVLLEKYAKGNEQTVADVRTR
                     VARALAAMENEDKRAHWEARFLEAQERGFVPAGRINSAAGTKLAATLINCFVQPVGDS
                     ITESVDGRCGIYTALAQSAETMRRGGGVGYDFSSIRPRGAMVRGTQSNASGPVSYMRV
                     FDRSCETVESAGSRRGAQMGVLRCDHPDIEDFIHAKDHGDLTNFNVSIAVTDLFMQAV
                     EAGTDVELAHKAEPASDIKAAGAYQREDGLWVYRKVPARDLWDQVMRSTYDHAEPGIL
                     FLDRMNRDNNLYYCETIEATNPCAEQPLPSYGCCCLGSINLTLYVKDPFTAKAAFDFA
                     AFGKTVETSIRMLDNVLEATHWPLEQQHAEAQSKRRVGLGFTGLGNALAMLCLRYDTT
                     EARAMATRISEFMRDQAYLASVELARERGAFPLFNADLYLSGGNFASRLPQDIKDKIR
                     KHGLRNSHLLSIAPTGTISLAFADNASNGIEPPFSYTYTRKKRMADGTFKEYAVEDYA
                     WRLYKHQGGDVNKLPEYFVTALEISAKAHKDMVAAVAPFVDTSISKTVNVPEDYPYAD
                     FEDLYLEAWKAGLKGLATYRPNSVLGSVLSVSTPSTEQKQPQDVEISDTNKRLSIKSL
                     PAPVLSSLRWPGRPDLPDGNAAWTYMLNTPQGNFALFVGHMDPIEDSPAKGFFPFEVW
                     VNGADQPRGLGAVAKTLSMDMRANDRGWLKLKLETLAKTVGEKSFEMRFPPHGEQKLM
                     PGVVSAFAQVVRYRCEKLGALEGDLPNPVLDTLFSLQEPKTGTDGTLSWTVDIQNPAT
                     GEDFVLGLKEITLPDGVTRPYSMWLSGNYPRALDGLSRILSLDMRVMDPAWIGMKLRK
                     LIDYPEPLGDFMAFVPGTRRQQNWPSTVAYMAALIIHRYAMLGVLDEAGYPTREMGIL
                     EAPRDDNEPKLMQGALCNECGNYSVIRKDGCDFCTACGAVGTCG"
     gene            complement(9373..10317)
                     /locus_tag="PKX01_11245"
     CDS             complement(9373..10317)
                     /locus_tag="PKX01_11245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HMZ76747.1"
                     /translation="MACLIAAPVAAESSAVVAFVGDVMLARTEATGRLIARGGDPFRR
                     VRSILAPADLRVANFESAGGRSGKADPAKPFSFRTTAAGVVAFSSVFEVAGLANNHAV
                     DFGRQALVETLASLRQSGSLGVGAGRDLVEAHRALIVERQGVRIGLLGYLDFFPRWFA
                     AAPGLPGVAWLDPRQVAVDIADARARGAEVVIVIPHWGEEHQPQANEHQRSRARAIID
                     AGADAVIGGHPHVVQDYEIYRGKPILYSLGNFVFDGFSDEDNITGWAVFATVDSAGVS
                     AIRTRVVRLDGHGAPAPDASKSGPCWTRGEAQMTRCAE"
     gene            complement(10338..10724)
                     /locus_tag="PKX01_11250"
     CDS             complement(10338..10724)
                     /locus_tag="PKX01_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76748.1"
                     /translation="MSVLRKPDVCFRTALLMALGLLSSAAALAVECTLEQGSMIRRMD
                     VPAGETSLAGQWATLGVFRVRTALTTPRQGRAWLLIEVYAEAGRQDFRILSSHTVESP
                     FDTGRVDVVAPRLGQSLTYTCKDDHP"
     gene            10984..11328
                     /locus_tag="PKX01_11255"
     CDS             10984..11328
                     /locus_tag="PKX01_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76749.1"
                     /translation="MDRIDARPEAFAHLVYRYLWPYAYFRDVTRGSFLERQQNYRHNR
                     RMGMHLPGFMLKWACLTVFFYLLGSLCEELLELVLPAACCYVTGAWTLTILVQLCVAW
                     LWLKRFPDIPRG"
     gene            complement(11368..12372)
                     /locus_tag="PKX01_11260"
     CDS             complement(11368..12372)
                     /locus_tag="PKX01_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HMZ76750.1"
                     /translation="MMLRTALLALILQLLALPAGAEGPIVIKFSHVVAADTPKGKASE
                     FFKRRAEELTRGRVKVEVYPNSSLYKDKEELEALQLGAVQMLAPSLAKFGPLGGKEFE
                     VFDLPYIFDNYADLHRVTQGPVGASILRQLDGKGIIGLAFWDNGFKSFSANTPIRTPA
                     DLKGKKLRIQPSKVLEEQVRALGALPQVMAFSEVYQALRTGVVDGTENPHSNMYTQKM
                     HEVQKYLCLTDHGYLGYAVIANKKFWDGLPGDVRSQLEQAMKESTVYANRIAQEENER
                     SLELIRKSGKTQIHVPSKEEREAFRRVLIPVHARMEARIGASLIRAIYRETGFDPNRP
                     "
     gene            12568..14544
                     /locus_tag="PKX01_11265"
     CDS             12568..14544
                     /locus_tag="PKX01_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76751.1"
                     /translation="MSQNPLNFRPPSPPPPSWYWGLPYVAVVLFIATVALLLWLTHRQ
                     DAEEQRGILINDVLWMEQNLSFQFERNAALLTQLGPELLGKIADDSTEARLKQALDPD
                     TGLVRILWLREDGRVKGAEPPFTDTHLVGEASGTFPSDATSRLARSMGRATYSDTYAV
                     IDNQAQFEVHVPVFERGRYVGAVVGVYSLNELLQHQIPWWFSERYRVTVTNNLGDEIA
                     AKSKVAPLSTLAYEIPFEPPGHGLSVSVTAYKSEVRWVPLLLMGSLVILGCAIVWSLW
                     ALRRHMRRRQAAEQALRAEHAFRKSMEDSLNTGMRARDLEGRITYVNPAFCRMVGFSA
                     DELIGMRPPMPYWPPEDLERILDVHAMILSGHAPSQGVEFRLMRRDGTRFDVLLIEAP
                     LIDPNGRHIGWMGSMVDVTEQKRAQELARQQQERLQATARLVTMGEMASTLAHELNQP
                     LAAISSYNTGCLNLIRQGDLPREELTGILEKIGRQAQRAAQIIRRVHDFVRRREPNFV
                     AADFNAILREAVELVEPTAERRGVSIDCDFTMPLPAVSADPVMIEQVAVNLIRNGMDA
                     MADTPIDERRLQLRTALRDGMVCLTVEDRGAGIPADVAEKLFAPFFTTKSEGMGMGLN
                     ICRSIAELHRGRLAYEAQPAGGTVFHFSVPVLQP"
     gene            14541..15155
                     /locus_tag="PKX01_11270"
     CDS             14541..15155
                     /locus_tag="PKX01_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76752.1"
                     /translation="MNTPLAHIVDDDEAIRDALAWLFRTRAVECRTWDSGEAFLDAWQ
                     PDWRGCIVLDIRMHGMNGLECFDALNARGNALPVIFLTGHGDVPMAVGALKAGAFDFL
                     EKPFKDNDLVDVVLRAIDSDTRRHASQATQADIAGRMGLLTPREHEVMQRVLAGKFNK
                     VIADELNISMRTVEVHRSRVFEKMGVRSAVELAQLLAPGREGRD"
     gene            complement(15152..16483)
                     /gene="tilS"
                     /locus_tag="PKX01_11275"
     CDS             complement(15152..16483)
                     /gene="tilS"
                     /locus_tag="PKX01_11275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMZ76753.1"
                     /translation="MPSTELVDQVARSWRRLPAPARKRLRVGLSGGLDSSVLLHVLAS
                     VRRAEGFGLSAVHVHHGLVPGADRWGRHCGVFCANLDVPLRVVDVVVDRESPAGIEAA
                     ARMARRAALLAEGGVVALAHHRDDQAETVLFRAVRGAGVRGLAGMREIQSQSDGLVWR
                     PLLNTYRAELEAYARAVGLVWIEDPSNMDHAHTRNYLRHELMPRLSVRFPGSVGTLAR
                     LGRLAGEASDLLDEMANEDRARLSCSDGSTRLAYAASLHLSSARWRNLLRSLLVDADP
                     ILPDEDRLREAERQFRSVGAPQGLRCRLGTQALCVYRDRWWLEGDSRDEGTPEGLALT
                     SLAALRWAGAALDFRAVTGAGIMRAALIGAPVRIDRRRGGERLSLHPAGPSRTLKNLF
                     QEAGIPPWLRPRLPVLWVGDRVAWVAGVGVAGEFRCPADAPGVVPEWNGLV"
     gene            complement(16470..17438)
                     /locus_tag="PKX01_11280"
     CDS             complement(16470..17438)
                     /locus_tag="PKX01_11280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HMZ76754.1"
                     /translation="MKTTFLEFEQPIAELEAKIEELRFVQDDSAVDISEEIARLEQKN
                     HGLTKDIYAKLTPWQCALVARHPQRPYTLDYVENIFTDFEELHGDRAFADDKAIVGGL
                     ARFNGQSCVVIGHQKGRDTKEKIFRNFGMPRPEGYRKALRLMRLAEKFGLPVFTFVDT
                     PGAYPGIDAEERGQSEAIGHNLFVMAELKVPIICTVIGEGGSGGALAIAVGDQVLMLQ
                     YSTYSVISPEGCASILWKSAERAADAAETMGITASRLKSLGLVDKVVSEPVGGAHRDH
                     RVMSQSLKRALQDALRQVSELSPTELLERRFERLMSYGQFKEIAVD"
BASE COUNT         3080 a         6020 c         5492 g         3008 t
ORIGIN      
        1 acttcctcgg acgagctggc gagggagtcg gcggtggtgc gcacttcggc gatgatggcg
       61 gcaagccgct cggacatgca cttgacgctg tagagcaggc tgctggtgtc gcccgccttg
      121 gcgagcactt ctgtggacag atcgccttcg gcaatcttgg ccacgacttc cgcggcgtag
      181 gccggctcac cgcccagttg ccgcatcagg ctgcgggtga tgaagaagcc gatcagacac
      241 accgccacca gggtgacgag gctgatggtg atggcctgcc agcgggcgat gccgagggtc
      301 tgggcggcct cgtcggcagc cttcctgccc agttcggcgt tgtactcgcg gtgctcttcg
      361 aaggcatccc agaggcgggc catgatggcc tggttggcca tgaccagatc ccgggcttct
      421 tccatcttgt tgacacggga cagcacatag acgcgctccc gcaactggtc gtagtccgcc
      481 agggcagccc ggtccgcggc gagcaggctc ttgtccttgt cgtcgctgat gagcggctcg
      541 taggccttga gggcttcatc caccttcttg cggttggcgc tgatgcgctc ttcgagggtg
      601 ctcatcaccg ccgggtccga cgcggcgatg tgctgccaca gctgggtacg agacgcggcc
      661 atgggactga aagccttatc cagggtctgg atgctgggca ccacattgac gttggcgtag
      721 ttggtgatct cgtagacccg gctcatctgg gtcaatccga gggcggccag gatgacgata
      781 ccgagcgctg cggacagcag cagggataac atttttttgc tgacggacat ggttggttct
      841 cctgagagag gctgccgggc tcacgccggg agcattgaga ggggggcgtt cttggcggcg
      901 gagtgacggg tgctgacctg gcgcacgagg cccggtacat cgatgatcag ggccacttcg
      961 ccactgccca gaatcgtgga accgccaatg cagcggatgt gactgaagac cttgccgagg
     1021 ggcttgatga cggtctggaa ttcgccgtag agctgatcca ccacgagtcc ggcgcgctcg
     1081 ccggcgtact ggaccaccac cacgttttcg cgccggggcg cttcaccgcc ggtgccgaac
     1141 agatcccgca ggcggatgaa gggcagcacc tcgccgcgca ggttgaggta gctggcgccg
     1201 tgctcgggac tttcgccggc cggcagttcg atgcattcca ccacctggtc gaggggcacc
     1261 acgtaggacg aggggccggc gccgatgagg aagccgtcga tgatggccag ggtgaggggc
     1321 aggcggatgc gcacggtggt gccctgccct tcgctgcttt ccaggtcgac ggtgccgcgc
     1381 agggcctgga tgttgcgctt gaccacatcc atgcccacgc cgcggccgga caggttgctg
     1441 acctgctcgg cggtggagaa gccgggctcg aagaccaggt ggaagatctc cttgtccgac
     1501 agggtctggc cttcggccac caggccgcgc tcgatggcct tgcgcaggat gcgttccttg
     1561 cgcaggccgc cgccgtcgtc gctgacttcg atgacgatgc tgccggagtc gtggtaggcg
     1621 ttgagggaca ggcgaccctg ggcggatttg ccggcggcga cgcgctgggc ggcgggctcg
     1681 atgccgtggt cgagggcgtt gcgcacgatg tgggtgagcg ggtcgccgat ccgttcgacg
     1741 acggtcttgt ccagctccgt gtcgccgccg cggatgtcca gctcgatgtc cttgcccagc
     1801 tcggcggcca cgtcgcggac gacgcggcgg aagcggttga aggtggcgcc gatctgcacc
     1861 atgcgcaggt cgagggcgga gtcgcgcacg ctttcgacga ggcgcgacag cagctcgctg
     1921 gcctcggcca gttccggcac cttgaggcgg cgggcgatga gctgggtgcc ggcgccggcg
     1981 atgatgagtt cgccgatgag gttgatgagc tggtcgagct tggcggcatc cacgcggatc
     2041 aggctggctt cgccgccctt ggcggtgccc tggccgcgga gctgctgctg gcgttcgacg
     2101 gcggcctcca ccagcggcgc atccacgagc tgctgctgga cgaggacttc gcccaggcgc
     2161 ggggcgggat cgccggcgac ggcgctgtcg gcggcctggc gatcgcgggt ggcgagggcc
     2221 tgggccagtt cgtcgcgggt gagggagccg cactgcacga gcatctcgcc gaggagctgg
     2281 cggtcttccg gcagggcgcc gagcagggcg cggtagtcgt ccagggaacc gtagggttcg
     2341 atgacgcgga cgatgctgtc ttcgcgcacg aaatcgaagg cgccgtcgat ggaatcccgc
     2401 ccggcggcgc tggcgaagct cagttcgaag cccaggtagc agctctccgg gtcgatcatg
     2461 gtgaggggcg gcacggcacc gaggaccatc tccaggctga cgatctgccc gagggtggac
     2521 aggtagtgga ggatggacag ggggtccatg ccgttgcgca gcaggtcggg cccggcgcgc
     2581 agggagatgt gccacaggcc gctgctgacg ccgtcgctgc gtacggaggt tgccacggcg
     2641 ggcaggtcct ggccgtcggg ccagtcgagg gcggccaggt cggcatcgac ctcggccact
     2701 gctgaccctg ccggcgctgt cgcatcggcg gacgcgggcg tggcggcgcc gtcgttcatg
     2761 acctccttga ggcgctggcc gaggacttcg ctgcgggcgc gcaggcccag gtccggcgcc
     2821 tcaccggcgg ccgcgcagct caccagggcg cccatgtgat cgctagattc cagcaacagg
     2881 ccgccgatag accgggtgag ggcgaggcga tggtcgcgga tgcggtcgag gaggctctcc
     2941 acgttgtggg tgaaggcgac gatgtcgtcc agcccgaaca gaccggccga gcccttgatg
     3001 gtgtgggcgg cgcggaagat ggcgttgagg gtgtccggat cgtccgtgcc gtgctccatc
     3061 tccagcagcg cttcctccat ctgctggagg agttcgcggc tttccacgat gaaggtctgc
     3121 agggcgtcgt cgagttgaat gctcatgatg tggatccgtc cctcagcgtg cgccggccag
     3181 ggcgtccttg aaaaagtcca tcaggcggca gaactcgaag gcctcgagga tgcgcaggct
     3241 gaccgccgaa aagcgcagcg cccgctccgc ggcccgggct tccagggcca gggaggccag
     3301 cagctggatg ccggcggcgt cgatgtcgct gacgccggac aggtcgagca ccacgtcgcc
     3361 cggcgcttcc atggcgatga gcaagcggtc gcgccagtcg cgggcggtgt agatggtcat
     3421 ttcaccggcg atgaccacgt gtgccgtgcc cagaatcgtc tgttccgatg cgttcatgtc
     3481 ggtctcctcg ggagtcgggg gcgttcaggg caggatcagc ttggccacgg ccgtgagcat
     3541 ctgctggggc tggaagggct tgaccaccca ggccttggcg ccggcggcct ggccttcgcg
     3601 cttcttgtcg tcgcctgcct cggtggtgag catgatcacg ggcgtgaagc ggtaggccgg
     3661 gacctgcttg agggccttga gaaaggtgat gccgtccatg ttcggcatgt tcacgtcgct
     3721 gatgatcagg tggatcttgc ggccgtcgag cttggtgagc ccgtcgcgac cgtcgcagcc
     3781 ttccacgacg tcgtatccgg cgccccgcag ggcgatggag acaacttggc gcaaggaggc
     3841 cgagtcatcc acgatcagga tggttttagc catgtcggtt cctcagaaga aagtgatgcc
     3901 ggcgtcttcc cgcgcggtca ccgggttggc gccacgttgc agcgcgtgtt gttcgggggt
     3961 ggtgaattgg cgcgccatgt cgcggcgcca ggcgatgggg tcaagggggt cgggatgcag
     4021 gccggcggcc agttcggcgc ggcagctgcg gacccgttgc tgcaggcggt ccatgtcacc
     4081 agagacgctg cccagcacct ggctgacccg gtcctggaac tgcagggcga ccagcacatc
     4141 gccgatctcg gtggcgacgg cgtggctctc ggcggacacc tcgcgggcgc gggccacgac
     4201 gcccccggcg gcgccgcgca ggcgctccac cacttcgccg atgcgccggc cggcgtcgct
     4261 gaccagctgc gcgtcgctct gggcataggc gcgggacacg gacagggcat cgcccatggc
     4321 gctggtgacg gtggcgatga tgtcgccgat cttcttgccg gtttcgccgg aggcggtgga
     4381 gagcttgcgg acctcgtccg ccaccaccgc gaagccgcgc cccgcttcgc cggcgcgggc
     4441 cgcttcgatg gcggcgttga gggccagcag gttggtctgc ttggccacgt cgcccacgtc
     4501 gcgggccatg ctctgcaggg acgaggtcag gcgggcgagt tcttccacct gaccgaggag
     4561 cacgtccttg ttctcgaggg cgcggcgcag ctcggcgacg atgccggaga gttcggcttc
     4621 gctggcattg aggatggtcg ccagattgcc gctgccatcg tcgctggcgc tgcccgcggc
     4681 cgattccacg cggcgggaca gatcaccgaa gcgaaccgac agggcggtga tggcctcctc
     4741 ggtctggctg cggaccatgc tcacctggtc gcgccacagg ggcaggacgt cctcgcacag
     4801 gcggtcgagg ccttcgaccg cggcgggcgg agcgtctgca gccggcccgt acgccggttc
     4861 gggcgtcggc agcagcggcc gggccgtacg ccaggccatc gccagcaccc ccagggctac
     4921 ggccagcgag gcgccgagca cctccaggcg gccgcccgcg gccagtgtcg gcacgcccgc
     4981 gaccagcacc gtcacgccgt accacaggat cggcatcgcg ccgaccgggc cttgcgcgcc
     5041 cgcttgcttc atcgtgtgag tctccatgtt caaaccccct gctccgttgc accactgcgg
     5101 tgacgccgcc actggccgcg aaaggccttc accagcgcca gcagctccgc ctcatccacc
     5161 ggcgtgcgca tcaacaccac gtcgtccggc agccgtgcgc gcacacccat gcagtccgcc
     5221 tccgaggcca ccagcacgat caccagcatc ccgcgggtcc gcgcgccctc caccagcgtg
     5281 ttgaccatcc ggaagaaatc gatcccggtc aggcccaggt cggagatgag catctccggc
     5341 gcttcgtcgc cgatgcgcag cagcgcatcg aagccgttcc ccgccgtcac caccacgcag
     5401 tcgggcaaca tgccttccag ctgctgcccc agggcttccc gcccggcttc gtcttcgcgc
     5461 agcaccagga tgcggaagcg cccacccggc ccgaacgcag caagccgctg ctcttccacc
     5521 agtcgctcca ccgactcccg caggatccgg cgatgcccac ccggcgtctt ccaggcccgc
     5581 agacgcccct cctccaccca caactgggca gtgcgtacgc tgattcccag gcgccgcgcg
     5641 gcgtcacggg tagagcaggt cagggaggtc gggtcgtctg ttttcatgat tgccgcgtcg
     5701 ttttgttaat aatactattt ttatcgtttt atacaagtat tttttttact ttttgtgttt
     5761 taggcgatct tgaagcgcat ccagcggaca tcgcctgccc cagacaactg ccattactat
     5821 ccgttttttc agtgttttag agcgcaaaac tgcatctttg cgggctcacg aacggaatcc
     5881 gaaggccgga gaacgcattt tgcgttttgc tgtttttttg tgttttcgat gtttttgacg
     5941 ggcaacacac cggcgtgagc ggcacggtgg agggccatcg acacaaggag gcggatgcgc
     6001 aggaacgcgg tcaggtcggc actggtcttc cagcaggcac gcccgcaagg cacgtcggcc
     6061 ccacgccggt gcattccctt gccaaatcaa ggacgtggcg cacacaggcg tcattcgctc
     6121 ttgattttcg accggaggag aactactaca tgtagtagta atttttgaaa tcagacacta
     6181 gctttatttt taagctattg tttttaaatg tttttatttt ttgccgaaat tccagcgtcg
     6241 gagtaagcta tgcgccgttc gacacatctc ccgatcacct ataaaaaggc cacgagacac
     6301 cctatgagcg ctatcgccaa agacgctgac gcccgcactg ccgccaccct cgcaccgcag
     6361 gagatctccg gcgaggtcct tctcgaaaag tacgccaagg gcaacgaaca gaccgtggcc
     6421 gacgtgcgca cccgcgtggc gcgggcgctg gctgccatgg aaaacgaaga caagcgcgcc
     6481 cattgggaag cgcgcttcct cgaagcccag gaacggggct tcgtgcccgc cgggcgcatc
     6541 aactcagccg ccggcaccaa gctggccgcc accctgatca actgcttcgt gcagccggtg
     6601 ggcgactcca tcaccgaatc cgtggacggc cgctgcggca tctacaccgc tctggcccag
     6661 tccgccgaga ccatgcggcg cggcggtggc gtgggctacg acttctcctc catccgcccg
     6721 cgcggcgcga tggtgcgcgg cacccagtcc aacgcctcgg gcccggtgtc ctatatgcgt
     6781 gtcttcgatc gctcctgcga gaccgtggaa tccgccggct cccgccgcgg cgcccagatg
     6841 ggcgtgctgc gctgcgacca tccggacatc gaggacttca tccacgccaa ggaccacggt
     6901 gacctgacca acttcaacgt ctccatcgcc gtcaccgatc tcttcatgca ggccgtggaa
     6961 gccggcaccg acgtggaact ggcccacaag gccgagcccg cttccgacat caaggccgct
     7021 ggcgcctacc agcgggaaga cggcctgtgg gtgtaccgca aggtgcccgc ccgcgacctg
     7081 tgggaccagg tcatgcgctc cacctacgac cacgcggagc ccggcatcct gttcctggat
     7141 cgcatgaacc gggacaacaa cctctattac tgcgagacca tcgaggccac caacccctgc
     7201 gccgagcagc ccctgccctc ctacggctgc tgctgtctgg gctccatcaa cctcacgctg
     7261 tacgtgaagg accccttcac cgccaaggcc gccttcgact tcgccgcgtt tggcaagacc
     7321 gtcgagacct ccatccgcat gctcgacaac gtgctcgaag ccacccactg gccgctcgag
     7381 cagcagcacg ccgaagccca gtccaagcgc cgcgtgggcc tcggcttcac cgggctcggc
     7441 aatgcgctgg ccatgctatg cctgcgttac gacaccactg aagcccgcgc catggccacc
     7501 cgcatctccg aattcatgcg cgaccaggcc tatctcgcct ccgtcgaact ggccagggag
     7561 cgcggcgcct tccccctctt caatgccgac ctctacctgt ccggaggcaa cttcgcgtcg
     7621 cgcctgcccc aggacatcaa ggacaagatc cgcaagcacg gcctgcgcaa ctcccacctg
     7681 ctgtccatcg cgcccaccgg caccatcagc ctggccttcg ccgacaatgc atccaacggc
     7741 atcgagccgc cgttctcgta cacctacacc cgcaagaagc gcatggccga cggcaccttc
     7801 aaggaatatg ccgtcgagga ctacgcctgg cgcctgtaca agcaccaggg cggtgacgtc
     7861 aacaagctgc ccgagtactt cgtcaccgcg ctagagatct ccgccaaggc ccacaaggac
     7921 atggtggcag ccgtcgctcc gttcgttgat acctccatct ccaagacggt gaacgtcccc
     7981 gaggactacc cctacgcgga cttcgaagat ctctacctgg aagcatggaa ggccggcctg
     8041 aagggtctcg ccacctatcg gcccaacagc gtgcttggtt cggtgctgtc ggtgagcacc
     8101 ccgtccaccg agcagaagca gccccaggac gtggagatct ctgacaccaa caagcgcctc
     8161 tccatcaaga gcctgcctgc gccggtgctg tcctccctgc gctggcccgg tcgcccggat
     8221 ctgccggacg gcaacgccgc atggacctac atgctgaaca cgccccaggg caacttcgcc
     8281 ctcttcgtgg gccacatgga ccccatcgag gacagccccg ccaagggctt cttcccgttc
     8341 gaagtgtggg tgaacggtgc cgatcagccc cgcggcctcg gcgcggtggc caagaccctg
     8401 tccatggaca tgcgcgccaa tgaccggggc tggctcaagc tcaagctcga gaccctcgcc
     8461 aagaccgtcg gcgaaaagtc cttcgagatg cgcttcccgc cccatggcga acagaagctg
     8521 atgcccggcg tcgtctccgc cttcgcccag gtggtccgct accgctgcga aaaactcggc
     8581 gccctcgaag gcgatctgcc gaacccggtg ctcgacaccc tgttcagcct gcaggagccc
     8641 aagaccggca ccgacggcac cctgtcgtgg acagtggaca tccagaaccc ggccaccggc
     8701 gaagacttcg tcctcggcct caaggaaatc accctgccgg acggcgtcac ccgcccctac
     8761 tccatgtggc tgtcgggcaa ctacccgcgc gccctggacg gcctgtcgcg catcctgtcc
     8821 ctcgacatgc gcgtcatgga cccggcctgg atcggcatga agctgcgcaa gctcatcgat
     8881 tacccggaac ctctgggcga cttcatggcc ttcgtgcccg gcacccgccg ccagcagaac
     8941 tggccgtcca ccgtcgccta catggccgcg ctgatcatcc accgctacgc catgctcggc
     9001 gtcctcgacg aagccggcta cccgacccgc gagatgggca tcctcgaagc tccccgcgac
     9061 gacaacgagc ccaagctgat gcagggtgcc ctgtgcaacg agtgcggcaa ctattcggtc
     9121 atccgcaagg acggctgcga cttctgcacc gcctgcggcg ccgtggggac ctgcggctaa
     9181 ggtcccgccg ccccgctggg gggccttcgc ctcagcgaac ggctccggtg tgggcggggc
     9241 aggcggtcat gctgaacagg acaacgcacc acgccaccgg caagccgtca aaacgcaacg
     9301 cccaccttga tcgaggtggg cgtttttcat gcggatcgcc gaacggtccg tccggacgtg
     9361 tggccgtaca ggctattccg cacagcgcgt catctgtgcc tcgccacgcg tccagcaggg
     9421 gcccgatttt gaagcatccg gcgcgggcgc accatgccca tccagcctga cgacccgggt
     9481 tcggatcgcc gaaacacccg cgctgtccac cgtggcgaac actgcccaac ccgtaatgtt
     9541 gtcctcatcc gaaaagccgt cgaacacgaa atttcccagc gagtacagaa tcggcttgcc
     9601 acggtagatt tcgtagtcct gaacgacatg gggatgacca ccgatgaccg cgtcggcacc
     9661 tgcgtcgatg atcgcccggg cccgcgatct ctgatgttca ttcgcctgcg gctgatgttc
     9721 ctctccccaa tggggaatga cgatcaccac ctcggcgcca cgcgctcggg catccgcaat
     9781 atccacggcg acctgacggg gatcgagcca cgcgacaccc ggcagtcctg gagccgcggc
     9841 gaaccagcgc ggaaagaagt ccagatagcc gagcagcccg atccggacgc cctggcgttc
     9901 cacgatgagc gcccgatggg cttcgaccag atcgcgtccg gcgccgacgc ccagcgatcc
     9961 tgactgacgc agggacgcga gggtctccac cagggcctgt cgtccgaaat ccaccgcgtg
    10021 gttgttggcc agtccggcca cctcaaacac agatgagaag gccactacgc cggccgccgt
    10081 ggtccggaaa ctgaagggct tggcgggatc ggccttaccg ctgcggccac ccgccgactc
    10141 gaaattggcg acccgaagat cggcgggcgc caggatgctg cgcacccgcc tgaaggggtc
    10201 gcccccccgc gcaatgagcc ggccggtcgc ctccgtcctg gccagcatga cgtccccgac
    10261 aaaggccacc acggcgctgc tctcggccgc caccggcgcc gcgatcaggc aggccacgaa
    10321 gccgagcccc agcctgttca tgggtgatcg tccttgcagg tataggtgag gctctgccca
    10381 agccggggcg ccacaacgtc cacgcgcccg gtatcgaatg gggattcgac cgtgtgggag
    10441 gacagaatcc tgaaatcctg gcggccggcc tcggcgtaga cctcgatcaa caaccaggcg
    10501 cgaccttggc gtggggtcgt caaggcggta cgcacccgga acacacccaa cgtcgcccat
    10561 tggcccgcga gcgacgtttc accggccggc acgtccatcc gccggatcat gctgccctgc
    10621 tccagcgtgc attcaacggc cagggcagcg gccgagctga gcagcccgag cgccatcaga
    10681 agggcggtcc tgaaacacac gtcgggcttt ctgagaacgg acatggaaag gcctgctttt
    10741 aacaggccgc attggtgggt tcggcttgtc acggtgcgcg aaaccggcag ccaagtcaat
    10801 tcagggccat gccgggagca ggatgctcgg gcggctcccg gcatcgtatc ggcagtctgg
    10861 gaggccctgc cagcgcgcgc cgtcgccacg caccgcgacg ccccttcact gaaccctcgg
    10921 cctgccgttg actcgaaggc attgcggccc ttcgcggctg cccaccagga agggaggatc
    10981 acgatggacc ggatcgacgc caggccggag gccttcgctc acctcgtgta tcgctacctg
    11041 tggccctacg cgtatttccg tgacgtgacc cgcggcagct ttctggagcg gcagcagaac
    11101 taccgccaca accggcgcat gggcatgcac ctgcccggct tcatgctcaa gtgggcgtgc
    11161 ctcacggtgt tcttctatct gctcggcagc ctctgcgaag aactgctgga attggtgttg
    11221 ccggcagcct gctgctatgt gacgggcgcg tggacactga ccatcctggt ccagctctgc
    11281 gtggcatggt tgtggctgaa gcgttttccc gacattccga ggggatgagg cagcgacggc
    11341 gcaggtgccg gatcgccgcc atccgtgtca ggggcggttc ggatcgaaac cggtttcccg
    11401 atagatcgcg cggatcagac tggcaccaat gcgggcctcc atccgggcat gcaccggtat
    11461 cagaacccgc cggaaggcct cccgttcctc cttgctgggc acgtggatct gcgtcttgcc
    11521 actcttgcgg atgagctcaa gagagcgctc gttctcttcc tgggcgatcc ggttggcgta
    11581 gaccgtcgac tccttcatcg cctgttccag ttggctgcgc acatcgcccg gcaggccgtc
    11641 ccagaacttc ttgttggcga tgacggcgta acccaggtag ccgtgatcgg tcaggcacag
    11701 atatttctgc acctcatgca tcttctgcgt atacatattg gagtggggat tttcggtccc
    11761 atccacgacc cccgtccgca gggcctgata cacctccgag aaggccatca cctggggcag
    11821 cgcgcccagc gcccgcacct gctcctcgag gaccttggag ggctgaatgc gcagcttctt
    11881 gcctttcaga tcggcgggcg tacggatggg cgtgttggct gaaaatgact tgaagccgtt
    11941 atcccagaag gccaggccaa tgatgccctt cccgtccagc tgacgcagga tggacgcgcc
    12001 caccggtccc tgggtgaccc ggtgcagatc cgcatagttg tcaaaaatgt agggcaggtc
    12061 gaagacttcg aattccttcc cccccagtgg gccgaacttc gccagggacg gcgccagcat
    12121 ctgtacggcg cccagttgca gcgcttccag ctcctccttg tccttataga gggagctgtt
    12181 gggatatacc tccaccttga cccgcccgcg ggtcagctcc tccgcgcggc gcttgaagaa
    12241 ttccgaagcc ttgcccttgg gcgtgtcggc cgccaccacg tgactgaact tgatgacgat
    12301 gggcccctct gcccccgcag gcagggccag gagctgcaga atcagggcca gcaatgcagt
    12361 acgcagcatc aacatgcggt cattccgtcc ataatcgccc ctgaaaagca tccacttgga
    12421 aacgtctgcc agattacaac ggaagacccg gatttcgaac agatccgcca gtactttctg
    12481 caagcttact ttccacccac ccgcccgttg tggaaactgc gaatggcatg agctgcccga
    12541 acggtcgata aaggagactc ccgactcttg tcccagaatc ccttgaactt ccgtccaccg
    12601 tcccctccgc cgccgagctg gtattggggc ctgccctatg tggccgtggt gctcttcatc
    12661 gccaccgtgg ccctgctgct gtggctgacc catcgtcagg acgcggagga gcagcgcggc
    12721 atcctcatca acgacgtgct gtggatggag cagaacctgt ccttccagtt cgagcgcaac
    12781 gccgccctgc tcacccagct gggtccggag ctcctcggca aaattgccga cgactccacc
    12841 gaagcccgcc tcaagcaagc cctcgatccc gacaccggcc tggtacgcat cctgtggtta
    12901 cgcgaggatg gtcgcgtaaa aggtgccgag cccccattca ccgataccca tctggtgggc
    12961 gaagccagcg gtactttccc ttccgacgcg acctcgcgcc tggcccgctc catgggccgt
    13021 gccacctaca gcgacaccta tgcggtcatc gacaaccagg cgcagttcga ggtgcacgtc
    13081 ccggtgttcg agcgcgggcg ctacgtgggc gcggtggtgg gcgtgtattc cctcaacgaa
    13141 ctgctccagc accagatccc gtggtggttc tcggagcgtt accgggtgac ggtgaccaac
    13201 aacctgggcg acgaaattgc cgcaaagtcc aaggtggcgc cgctgtccac cctggcctac
    13261 gagattccct tcgagccacc cggccacggc ctgtcggtga gcgtcaccgc ctacaagagc
    13321 gaagtacgct gggtgcccct gctgctgatg ggctccctcg tcatcctggg ctgtgccatc
    13381 gtgtggagcc tatgggcgct gcggcgtcac atgcgccgac gacaggccgc ggaacaagcc
    13441 ttgcgcgccg agcacgcctt tcgcaagtcc atggaggatt ccctcaacac cggcatgcgc
    13501 gcccgcgacc tggaaggccg catcacatac gtcaatccgg cgttctgccg catggtgggc
    13561 ttcagcgcgg acgagctcat cggcatgcgt ccgcccatgc cctactggcc accggaagac
    13621 ctggaacgca tcctggatgt acacgccatg atcctgtccg ggcacgcacc gtcccagggg
    13681 gtggaattcc gcctcatgcg ccgggacggt acccgcttcg atgttctgct gatcgaggcc
    13741 cccctcatcg accccaacgg ccgccacatc ggctggatgg gttcgatggt ggacgtcacc
    13801 gaacagaagc gcgcccagga actggcccgc cagcagcagg aacgcctgca ggccacggcc
    13861 cgtctcgtca ccatgggcga gatggcctcc accctggccc acgaactcaa ccagcccctc
    13921 gccgccatct ccagctacaa cacgggctgc ctgaacctca tccgccaggg cgacctgccc
    13981 cgcgaggaac tcaccggcat cctcgaaaaa atcggccgcc aggcccagcg cgcggcccag
    14041 atcatccgcc gcgtgcacga tttcgtacgc cgccgcgaac ccaacttcgt tgcggccgac
    14101 ttcaacgcca tcctccggga agcggtcgaa ctggtcgaac ccacggccga acgccgcggc
    14161 gtgagcatcg actgcgactt cacgatgccc ctgccggcgg tgagtgccga tccagtgatg
    14221 atcgaacagg tggcggtcaa cctcatccgc aacggtatgg acgccatggc cgacacgccc
    14281 atcgacgagc gccggctgca attgcgcacc gccctgcggg acggcatggt ctgccttacg
    14341 gtcgaagacc ggggcgccgg cattcccgcg gacgtggccg aaaaactctt cgcccccttc
    14401 ttcaccacca aaagcgaagg catgggcatg ggcctcaaca tctgccgctc catcgccgag
    14461 ttgcatcggg gtcgcctggc atacgaagcc cagccagccg gaggcaccgt tttccacttt
    14521 tccgttccgg tcctgcagcc atgaacacac cccttgccca catcgtcgac gacgatgaag
    14581 ccatccgcga cgccctcgcc tggctgttcc gtacccgcgc cgtcgaatgc cgcacctggg
    14641 actccggcga agcctttctc gacgcctggc agccggactg gcgcggctgc atcgtgctgg
    14701 acatccgcat gcacggcatg aacgggctgg aatgcttcga cgccctcaat gcccgcggca
    14761 acgccctgcc ggtgatcttc ctcaccggtc acggcgacgt acccatggcg gtcggcgccc
    14821 tcaaggccgg cgccttcgac tttcttgaaa aacccttcaa ggacaacgac ttggtggacg
    14881 tggtcctccg cgccatcgac agcgacacac gccgccacgc cagtcaggcc acccaggccg
    14941 acatcgcggg acgcatgggg ctgctcaccc cgcgtgaaca cgaagtcatg cagcgcgtgc
    15001 tggccggcaa gttcaacaag gtgatcgccg acgaactgaa catctccatg cgcacagtgg
    15061 aggttcatcg ctcacgggtt tttgagaaga tgggcgtgcg ctcagcggtg gaactggccc
    15121 agttgctcgc gcccggccga gaaggacggg actagaccag tccgttccac tcgggcacga
    15181 cgccgggcgc atcggccggg cagcggaatt cgcccgccac accaacgccc gccacccagg
    15241 cgacccggtc gccgacccac aacacgggca gacgcggccg cagccacggc ggaatgccgg
    15301 cctcctgaaa gagattcttg agggtgcgcg acggacctgc cgggtgcagg gacagacgtt
    15361 cgcccccacg ccgtcggtca atccgcaccg gcgcaccgat caacgcggca cgcatgatcc
    15421 ccgcccccgt cacagcccga aaatccaagg ccgccccggc ccacctcagc gcggccaaag
    15481 acgtcagtgc aagaccttcg ggcgtgccct cgtcacgaga atcgccctcc agccaccagc
    15541 ggtcacggta tacacacagc gcctgtgtcc cgagccggca acgcagacct tgaggggcgc
    15601 cgacgcttcg gaactgacgc tccgcctccc gtagccggtc ttcgtcgggc agaatcgggt
    15661 cagcgtccac aagcagagat cgcagcagat tgcgccagcg cgccgacgac aggtgcaaag
    15721 acgcggcata ggcaagacgc gtcgatccat cgctacagct cagccgcgca cgatcctcgt
    15781 tcgccatctc gtccagcaga tccgatgctt cacccgccag acgcccaagc cgagccaacg
    15841 tcccaacact ccccgggaaa cgtactgaca gccgcggcat cagttcatgg cgcaggtagt
    15901 tgcgggtgtg ggcatggtcc atattgctcg gatcctctat ccataccaga cccacagccc
    15961 gggcataggc ttcaagttcg gcacggtacg tgttcagcag agggcgccac accagcccgt
    16021 cggactggga ctggatctcc cgcatccccg cgagccctct gacgccggcg ccgcgcacgg
    16081 cgcggaacag caccgtttcg gcctgatcgt cccgatgatg ggcaagggcg acgaccccgc
    16141 cctccgccag cagcgccgcc cggcgcgcca tccgcgccgc cgcctcgatc ccggcgggac
    16201 tctcgcggtc gaccacgacg tcgaccaccc tgagcggaac atccagattc gcgcagaaca
    16261 cgccacagtg cctaccccac cgatcggcac caggtaccag tccgtggtgg acgtgcacgg
    16321 ccgacaggcc gaatccttcg gccctacgca cgctcgcaag cacatgcagc aatacggaag
    16381 aatcgagccc cccgctcaga ccaacacgca gcctcttgcg cgccggagcg gggagccgcc
    16441 gccacgaacg ggcgacctga tcgaccagtt cagtcgacgg caatttcctt gaactggccg
    16501 tagctcatca gacgctcgaa gcgtcgctcc agcaactcgg tcggcgacaa ttcggacacc
    16561 tggcgcaaag catcctgcag agcccgcttc agactctgac tcatcacccg atgatcacga
    16621 tgggcgccgc ctaccggttc ggacaccacc ttatccacca gacccaggga tttcagccgc
    16681 gacgccgtga ttcccatggt ttccgctgcg tccgccgcac gttcggcgct cttccagagg
    16741 atggacgcac acccttccgg agagatcacg gaataggtgg agtattgcag catcagcacc
    16801 tgatcaccca cagcaatggc cagcgcgccg cccgagcccc cctcgccgat cacggtgcag
    16861 atgatgggca ccttcagctc ggccatcacg aacagattat gtccaatggc ctctgactgc
    16921 ccgcgctctt ccgcgtcaat gccgggatag gcgcccggtg tatccacaaa ggtgaagacc
    16981 ggcagtccga acttctccgc aagacgcatc agacgcagcg ccttgcgata gccttccggg
    17041 cgcggcatcc cgaagttgcg gaagatcttc tccttggtgt cgcgcccctt ctgatggcca
    17101 atcacgacac aggactgccc gttgaagcgg gccaaacccc caacgatcgc cttgtcatct
    17161 gcaaaggcgc gatcaccgtg gagttcctcg aagtctgtga agatgttctc aacataatcg
    17221 agggtgtagg ggcgctgcgg atgccgcgcc accaaagcac actgccaggg ggtcagcttt
    17281 gcgtagatat ccttggtaag gccatgattc ttttgctcga gtcgggcgat ttcttcggag
    17341 atatccacag cggaatcatc ctgcacgaaa cgcagctctt cgatcttggc ttccagttcg
    17401 gcaatgggct gctcgaactc gagaaaagtg gtcttcatct gcagagctcc cgacgataat
    17461 ttgagattct accctaagga aggtcgagct cttccttgcg atgcagctca gcgcatggcc
    17521 gctgtacatt ttccttctta ttttccatag gcaaaccccc gaccaatgat ttggccgggg
    17581 gtctgatggg gttagtctga
//
LOCUS       DAOQPA010000077        17581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1623_length_17581_cov_19.006562, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000077 DAOQPA010000000
VERSION     DAOQPA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17581
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1623_length_17581_cov_19.006562"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..611)
                     /locus_tag="PKX01_11285"
     CDS             complement(396..611)
                     /locus_tag="PKX01_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76755.1"
                     /translation="MLNKFIALAIASAFATASFAEAPAAAPAAAPAAAATAPAAEPAA
                     KPKAKKHHHHHKAAAPAAPAAEAAPAK"
     gene            complement(746..1141)
                     /locus_tag="PKX01_11290"
     CDS             complement(746..1141)
                     /locus_tag="PKX01_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HMZ76756.1"
                     /translation="MPIIQLKISAPKSPEMTHRIAGMLLGLTSRILHKKPEVTAITID
                     YVVPEDWIVGGRTLAEQRRHSFFFDIRITDETNTKDEKARYIREAFDAFAGLLGDVHE
                     ESYIHVHDVRAASYGYGGRTQEFRYHRPG"
     gene            complement(1193..1609)
                     /locus_tag="PKX01_11295"
     CDS             complement(1193..1609)
                     /locus_tag="PKX01_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ76757.1"
                     /translation="MFDQCLYFNTTALARRLEREWADAFRPFGLTPPQAFLLRAVVAE
                     PGRCPGELAAFLAISRPTATRALDGLAAKGLVERRSSPRDGREATVFPTAEAERIGPA
                     VNEAAGAVTTRLKTVLGEAVFVDALAQVRSVRGALG"
     gene            1782..3785
                     /locus_tag="PKX01_11300"
     CDS             1782..3785
                     /locus_tag="PKX01_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HMZ76758.1"
                     /translation="MKLHAFVAMPFGTKPDAAGNPVDFNAVYDDLIRPALEKAGLDVF
                     RADEEPRAGDIRADMFQELLMADLVLADLSIDNPNVWYELGVRHALRARGVILLQAPR
                     DRQPFDIYTDRKLHYRLKDGRPDPATLDDDRKAITDMATATLKAWHGRKVSPVYALLP
                     NLQEPDWKSLRIGDAREFWERHDAWCALIERARDDNRPEDILVLADEAPITALRVEAR
                     LAAGNALLQGRHFNFALEQFDLAHRFDPDNLTVSRKRGVCLQRTGRAAEAQDLYRDIL
                     AAHPDDVETWSLLGRVDKDAWVTAWRTPGSTPEAMRDAATYEDALLRAAIDSYAAAFR
                     AVPGHFYSGINAVTLMALYQDLTGDTRYAADQAVMAGGIAWSAHSTLTARPCGQAPQD
                     LYWAAATLGDLAVLRGDAPAVRAAYKEAIVHADNDWFALDSTLGQLRLLAELGFRPDN
                     VAAGIATYERALARLRPPEKSWQPGKVFLFSGHMVDAPGRKEPRFPADKEPVAAAAID
                     QALSDEGASDQDLALTQGAAGGDLLFAEAALKRGLRLQLLLPLPEPDFIEASILRSAD
                     GETWRKRYFQVRDHALCLPPRVMPDVLGPLPVDHLGNSMNEFERCNQWLLYSALACGI
                     ARTRFVCLWNGTGGDGPGGTQHMVQEVRRRTGKVTWLDTRKLW"
     gene            3808..4299
                     /locus_tag="PKX01_11305"
     CDS             3808..4299
                     /locus_tag="PKX01_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76759.1"
                     /translation="MLRPLPLLSLAALLLFVGVLWSDQPLVEAGHSMVELQFAGSAER
                     AARILDTWRTAGVEDAIRLNQQIDFAFLLTYPAALSLACRSLAGHLGPGQRIGHFLTR
                     AVFACTVLDGAENLAIQVMLDHGARDSMARLATACAIPKFALAAWAIGWCLWALLRRV
                     RLP"
     gene            4296..7106
                     /locus_tag="PKX01_11310"
     CDS             4296..7106
                     /locus_tag="PKX01_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HMZ76760.1"
                     /translation="MSTQAHGHPHGPRQRRSFAARLFGYDLFISFALGHPPRGTQSYA
                     SDLARRLRERDFAVFYSEDEAAPGGELDSTLRNALARARILVVIANRGTLADPRWVRR
                     EVEEFRALHPGRPIIPISLDGALQDPQLAAACKDWLDMSDTIWLDDTAEAAEHGLVSP
                     AVIDRLVLAPTQMRSSQGWRITVAAVSLTLIGLTASSIWSEHRARTNENSARQEAEHA
                     RRAERQAREELRVSTGLRLRAESSAMLGGLRPEPDERALLQLLAALHLGRGDDTLDPD
                     AEGELLGGLRHGRNLVRMSPAAHPILSLALSRDGTHLVTGGEDATLQLWNALTGDPVG
                     PPLRAHEDAVRAVDFSPDGQWLVSASTDNTLRLWQVGRDGLQPAPLPPLKGHTDSVRT
                     VAFSPDGRLIASGSDDNTVILWDARSGTPLPRRLTGHGDSVTGLAFLPDGRRLVTASN
                     DMSLRLWDIPSGRPLGPPLSGHTDSVKCVAVSPDGRRIVSGAWDNTLRLWDPVTRRPI
                     GRPLTGHRALVRSVAFSADGRRIVSSSDDGTLRMWDARRGTLLAPPLRGHQGEVTSAL
                     FARKGQRIISGGMDRSLRQWDPRTTSPLGEAQSAGSAALLSVAVSPDGKRLAGSGEDL
                     RGRLWRVASARTAANARGIALSGPSAAFYALEFNADGARLAGGDAAGQLWQWDGRSGE
                     TLAAPHPAHARPISALAYSPDSRRIATGSWDNTIRIWDATSGAPLGPPLTGHRGWIAA
                     LAFSPDGRTLVSASADTTLRRWDVATGHLLGAPLSGHSNWVLAVAFSPDGRNLASAGA
                     DRQIRRWNAVTGAGIGTPSGGHESWITSLAFSPDSRYLVSAGWDRSLRLWRTDSGLPV
                     GIPLQGHRDWIQKVAFRVDGLRIHSADRAGALHLWAAPRHWPEMLCAKLSRNLSRAQW
                     KAWVSPDIDYVVQCPGLPVPPD"
     gene            7128..7505
                     /locus_tag="PKX01_11315"
     CDS             7128..7505
                     /locus_tag="PKX01_11315"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HMZ76761.1"
                     /translation="MHEPADIHRLFAERFAAADLDGLLELYAPDALLLPQAGSEVRGH
                     AAIREALGGFIALGGRFTMAPPRCLRAGDIAVLHADWQLSASGPDGQPVHLGGHTADV
                     VQRQVDGTWRLIIDNPWGDAVTA"
     gene            7627..8784
                     /locus_tag="PKX01_11320"
     CDS             7627..8784
                     /locus_tag="PKX01_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ76762.1"
                     /translation="MTQTLADRIRAPGPKKILSCDGGGILGLISVEILAKLEADLRAR
                     TGKPDLLLGQWFDFVCGTSTGAIIAACIAAGMSMERIRTFYIESGQQMFDKASLFRRL
                     QYSYNDEPLARKLRDEFTRALGDDTTLGSPGLRPLLMMMMRNATTDSPWPVSNNPFGK
                     YNQPARKDCNLRLPLWQLVRASTAAPTYFPPEVVTLGQGDDAYSFIFVDGGVTTYNNA
                     AFMAFQMATATPFGIRWATGTREMLIVSVGTGSVAAARPDLEADDLTLIENAKTLPGA
                     LMNAAAAGWDRACRLIGECRFGALIDSEFGDMVVPPGGANTYSGSKQFAYVRYDPDVS
                     QAGLNALGLSGIKAADVQTLDSVAHIPDIRTVGKTYADRNVKLEEHLRGFV"
     gene            8866..10314
                     /locus_tag="PKX01_11325"
     CDS             8866..10314
                     /locus_tag="PKX01_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4071 domain-containing protein"
                     /protein_id="HMZ76763.1"
                     /translation="MTPKTCFVIQGFGKKTDYTDGRVLDMDASYGVIKDAVEAAGLVC
                     LRADEIPHSGTIDEPMYRQLLQADLVIADLSTYNVNAAFELGVRYGLRPRATLIVAEE
                     GFKNPFDVSHLVIRRYKHLGEDIGVKEARRFADELKLAIAAILSADQADSPVYTFLPG
                     LQPPALPPPAPRAAPVQAQAEAPTRAGGFDLSKARDWLDGLFHTSAPEPGTDAAADTA
                     GPPTLSRWEPPEVPAPAAPRSAKSLLDEALAALQPGEDTPPDFIRARRLLEAVREQRP
                     NDPFVIQQLVRAISGAARPTPQAALREALALMNELGPATTNDPETLSLWGSVHQRRWE
                     LDAQPADLEEAIAGFERGYFVKQDWQTGSALAFLLDRRALEHLKSGASDEAVADGVQA
                     RRLRREVLRLAGPHIDHPADDAAKRFSVMCAVWEAHLGLGARSEADMLDARLDALPVP
                     APLNARRKAHGRTLVAQLDAFTRLRGGAAGST"
     gene            complement(10557..11372)
                     /locus_tag="PKX01_11330"
     CDS             complement(10557..11372)
                     /locus_tag="PKX01_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ76764.1"
                     /translation="MPAEVVSVAFDCLPRLAPSADEPVTVNQRRVPPDVAVPPHRHPW
                     GQLAYPVSGALRIAAAGIAWIVPPFRAVWIPPEMEHDVVMIGQVDLRTLYIAREVAPL
                     ALDRCAVVDVSPLLRNLIEALAERRTDRRHRLMMELVLEEIRVAPPLSLGLPLPKDRR
                     LKQLCDRLMADPADDRGLAEWAREVAASERTLARLFRAELDTSFGAWRQQLRLARAVD
                     DITRGVPLARVAAEVGYASQAAFSAMFRRALGVPPSRFSGAPKERRGGDPEQG"
     gene            11508..12710
                     /locus_tag="PKX01_11335"
     CDS             11508..12710
                     /locus_tag="PKX01_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ76765.1"
                     /translation="MSSDAALSAPPRHDAFVIALIGSVHGVSHFFHLLLPPLFPWLMP
                     EFGLDYSRIGTTMTVFFVVSGVGQALAGLAVDRFGPLRVLASGIGCFVLAGLTLSRAN
                     GFADLMIVAALAGLGNSVFHPCDFTVLNRNVSVPRLGHAFSVHGLSGNVGWALAPLFM
                     TGIATAAGWRTAALAAGLLAIPALALLLAGRRHLVDATPPEHQDGRRASTAEILRVGP
                     VWLCFAFFFFLTTAFGAIQAFGTPLMQHLYELPLGIAAGTVSSYLFAGAVGIFVGGFL
                     ARSVQQDRVIALALSAAAAMAVLLASAQVPAGAVLPVMACIGFLSGLAGPSRDLLVRR
                     AATARFGQAAFGRVYGFVYSGLDTGLALAPLVFGGFMDSGHYGTVLVGIALLQVAAVF
                     TALKVGRG"
     gene            12710..13282
                     /locus_tag="PKX01_11340"
     CDS             12710..13282
                     /locus_tag="PKX01_11340"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HMZ76766.1"
                     /translation="MARIFSFPPVAAPDARVLILGSMPGEASLKARQYYAHPRNLFWP
                     ILGELVGARPDLPYPDRLAALRQAHIALWDVLQSCERDGSLDSAIDPQTRVVNDFGDF
                     FTRHPHIGWIFFNGREAEKTFRKHAIHVCDAERFRYTLLPSTSPANASIPPAVRLDAW
                     RVIVDAPRRVSAPHPGSMAGAGATLQGSST"
     gene            complement(13264..14997)
                     /gene="phaC"
                     /locus_tag="PKX01_11345"
     CDS             complement(13264..14997)
                     /gene="phaC"
                     /locus_tag="PKX01_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HMZ76767.1"
                     /translation="MSSREPPAPFSTARKSLKKIHDSVEASIDPIGMTAPIVHAQLAW
                     MAHPQELADALAKVSTSLWALQWHSMRRVFGLPSEDPEKPHGDDTRFADPVWGESPTW
                     DLIKEWYLVFTHHMQDMLYDTPGLSQKERRRAAFWWRKWLNAMAPTNFLMTNPVALRL
                     AMETRGESLVKGFQNFLADMEAGNVRMTDPNDFQVGVNLATTPGKVVFRNCLLEVIHY
                     TPSQPKIHARPVVIVTPWINKFYILDLTSRKSMIRYLLDQGLDVFITSWKNPGEDMRD
                     VRFDDYLTEGVHMLVETARAMSGADKVHAVGYCIGGTVLTTYMAWANRHFKAEDVPVA
                     HFTLFTTLVDFHKPGDIEVFVDEGSIRYLTQTMAQKGYLDGAEMASAFRLLRSNSLIW
                     HYVVHGWLYGEKPAPFDVLYWNMDSTRMPYAMHAWYLRELYLHNRLIEKDALTVAGEP
                     IDLGAIAQPVYAVAAEDDHIAPWQQTFRTMNYVAGPKRYVLSSSGHILGIVNPPVNPP
                     KRRYHVGPARRSDAADGWLAKAAEHPGSWWTDWMAWLKPQCGDLVDARPVADEAHPAL
                     ADAPGTYVLEP"
     gene            15167..15610
                     /locus_tag="PKX01_11350"
     CDS             15167..15610
                     /locus_tag="PKX01_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ76768.1"
                     /translation="MFKHILVPTDGSELSSATVTRAVTFAREAGAKITFFYAQPDFPM
                     PIYGEGALIDPTTPEQFSKAAAAEADAILAKSRELATVAGVECAGVTVVSEVPYEAII
                     EAAEKSACDLIFMASHGRRGLSGFLLGSETQKVLTHSKIPVLVYR"
     gene            complement(16099..16341)
                     /locus_tag="PKX01_11355"
     CDS             complement(16099..16341)
                     /locus_tag="PKX01_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76769.1"
                     /translation="MRKLIEVLWPSFLVAGLAEIVFFTVVNPQELYLFGEPVHFSATA
                     TYSIGFFGFWLVCAASSLMTLFFQRTSAEINKLDGV"
     gene            complement(16401..16544)
                     /locus_tag="PKX01_11360"
     CDS             complement(16401..16544)
                     /locus_tag="PKX01_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HMZ76770.1"
                     /translation="MAWKELFSTDIGMLSLFTIGFVIVMGGYLYRYAKSHMAEDERRA
                     RGG"
     gene            complement(16559..17092)
                     /locus_tag="PKX01_11365"
     CDS             complement(16559..17092)
                     /locus_tag="PKX01_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMZ76771.1"
                     /translation="MNTSTLNTIRDDKPWYRERWPWLLMAMPATAIVAGFITLWLAIT
                     SFDGLVADDYYKQGLAINQTIGRAVVARDLGLVAQVRFSADGVAIALTAKPGIALPER
                     VLVTIAHPTRGGLDQHLNLQGQAGAYRGALAQELVSAHWKVLLEDESRSWRLTGTAFL
                     PTETEIRLDSADLKPVD"
     gene            complement(17089..>17581)
                     /locus_tag="PKX01_11370"
     CDS             complement(17089..>17581)
                     /locus_tag="PKX01_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HMZ76772.1"
                     /translation="PKGLIRYTTENAMRRHWGRKEILGHVMRPRILIYSSVLVALVIA
                     LVWGLATRSTLRVDVIKDRSTLSREVEGGLIANVYNLQVMNMTEKPRTFTLSVSGLDQ
                     IRISPNKPFEVDAAGLKAVTVEVAAPPESGKPGSNTIYIEVKAADDQTVAVREKTAFL
                     LPQ"
BASE COUNT         2908 a         6434 c         5381 g         2858 t
ORIGIN      
        1 cgggcagccg ctccgatccg tcaaactcgg cctagcaacc gtgcggcgaa accgctccag
       61 gccggaacgc acggccccgc tgcacatctg ccggattcag acggcgtcgc cgtcatcacg
      121 cgcacctcgc ggcctcgtcc ccgcgggcgc agggtcgcca cgctgtcgat ccggaacggg
      181 tcatcggcac ctcgtgcgcc gcgtcctgcc ggcgcactgc ccccgcatgt cctggcgcgt
      241 tgcctgattc acgggcatca gcctgcagcg ggacgggcgc ccatcgccgg aggtacgtgc
      301 atcggaatgc gcaaaaagac agggcccgga aacaaggttt ccgggccctg catccttccg
      361 cgccggccag tccggcgcgg gaaggcttcg tcgacttact tggcgggagc cgcttcggca
      421 gccggcgccg ccggggccgc tgccttgtga tgatggtggt gcttcttcgc cttcggcttg
      481 gcggcgggtt cagccgccgg ggcggtcgcc gcagccgcag gcgctgctgc cggcgcagcg
      541 gccggtgcct cggcgaaaga agcggtcgca aacgcggaag caatggccag ggcgatgaat
      601 ttgttcagca tgttctgttt ctcctagatc ggatcacgat gttgtggggt tggcagaatc
      661 accagcctca agcagaacaa cgcgccaccc tgccagagcg tgacagacga agtgtaagca
      721 aacattccac cccgccgcat ccacgtcacc ccggcctgtg atagcggaac tcctgggtgc
      781 gcccgccgta cccgtaggac gcagcgcgca catcgtgcac atggatgtag ctctcctcat
      841 gcacgtcacc cagcagtccc gcaaaggcat cgaaggcctc gcgaatgtag cgggcctttt
      901 cgtccttggt attggtctcg tcggtgatgc ggatgtcgaa gaagaagctg tgccgccgct
      961 gctccgccag cgtccgcccc cccacgatcc agtcttccgg caccacgtag tcgatggtga
     1021 tcgccgtcac ctctggcttc ttatgaagga tgcggctcgt caggccgagc agcatcccgg
     1081 caatgcgatg ggtcatttcc ggagacttcg gagcactgat cttcaactgg atgatgggca
     1141 tacacgcctc cattctgatt agccacacaa ctatataagc gaacaaaaac cgctacccca
     1201 gcgcaccccg cacgctccgc acctgggcga gcgcatcgac aaacaccgcc tcgccaagca
     1261 ccgtcttcag acgggtggtg accgcgccag ccgcctcgtt caccgccgga ccgatccgtt
     1321 cggcctccgc cgtggggaac accgtcgcct cccgtccgtc ccgcggcgac gaccgacgct
     1381 ccaccagccc cttggccgcc agcccatcca gggcacgggt ggccgtcgga cgggagattg
     1441 ccaggaacgc cgccagttca ccgggacacc ggccgggctc ggccacgacc gcccgcagca
     1501 ggaaggcctg gggcggcgtc aggccgaagg gacggaaggc gtcggcccat tccctttcca
     1561 ggcggcgcgc cagggctgtc gtgttgaaat acaggcattg atcgaacatg gctgcatggt
     1621 agtacgattt aattcgctat gcaatcatat tccggtggag tccatcctct ggggggccgg
     1681 tacatccccc gccaccttgg cagggcgaag cctgccgaac taagatggga catgccatcc
     1741 gatccgcatg gatccccttc ctgaccccga aagcccccaa cttgaaactg cacgccttcg
     1801 tcgccatgcc ctttggcacc aaaccggatg ctgccggcaa tcccgttgac ttcaacgccg
     1861 tctacgacga cctgatccgc cctgccctcg agaaggccgg gctggacgtg ttccgcgccg
     1921 atgaagagcc gcgcgccggc gacatccgcg ccgacatgtt ccaggaactc ctgatggccg
     1981 acctggtgct ggccgacctg agcatcgaca atcccaacgt ctggtatgaa ctgggcgtgc
     2041 gccacgccct gcgcgcccgc ggcgtgatcc tcctgcaggc cccccgcgac cgtcagccct
     2101 tcgacatcta caccgaccgc aagctccact accgcctcaa ggacggcagg ccagacccgg
     2161 cgaccctgga cgacgaccgc aaggccatca ccgacatggc caccgccacc ctcaaggcct
     2221 ggcacggacg caaggtcagc ccggtgtacg cccttctccc caatctgcag gagcccgact
     2281 ggaagagcct caggatcggc gacgcccgcg agttctggga acgccacgac gcctggtgcg
     2341 cgctcatcga gcgggcccgc gacgacaacc gtccggagga catcctcgtc ctcgccgatg
     2401 aagcgcccat caccgccttg cgtgtggagg cccgcctcgc ggccggcaac gccctgctcc
     2461 agggccggca cttcaatttc gccctggaac aattcgacct cgcccaccgt ttcgacccgg
     2521 acaacctcac cgtcagccgc aagcggggcg tctgcctgca gcgcaccgga cgcgccgccg
     2581 aggcccagga tctgtaccgg gacatcctgg ccgcccaccc ggacgacgtg gagacctggt
     2641 ccctcctggg ccgcgtggac aaggacgcct gggtcaccgc ctggcggacg cccggcagca
     2701 cacccgaagc catgcgcgac gcggccacct acgaggacgc cctgctgcgc gccgccatcg
     2761 acagctacgc cgccgccttc cgcgccgtgc ccggtcactt ctactcaggc atcaacgccg
     2821 tcaccctgat ggccctctac caggatctga ccggcgacac ccgctacgcc gccgaccagg
     2881 ccgtgatggc cggcgggatc gcctggtcgg cccattccac cctcacggcc cggccgtgcg
     2941 gccaggcgcc acaggatctc tactgggcgg ccgccaccct gggcgacctg gccgtcctgc
     3001 gcggcgacgc cccggcggtc cgcgccgctt acaaggaagc catcgtccac gccgacaacg
     3061 actggttcgc cctcgactcc accctcggtc agctccgcct gctggcggaa ctgggcttcc
     3121 ggcccgacaa cgtggccgcc ggcatcgcca cctacgagcg tgcccttgcc cgcctgcgcc
     3181 ccccggagaa gagctggcag cccggcaagg tcttcctttt ctccggtcac atggtcgatg
     3241 cgccgggccg caaggaaccc cgcttcccgg ccgacaagga gcccgtcgcc gccgccgcca
     3301 tcgaccaggc gctgagcgac gagggcgcca gcgatcagga cctcgccctc acccagggcg
     3361 ccgcaggcgg cgacctgctc ttcgcggaag ccgccctgaa acgcggcctg cgcctccagc
     3421 tcctgctccc cctgcccgag ccggacttca tcgaagcctc catcctccgc tccgccgatg
     3481 gcgagacctg gcgcaagcgt tacttccagg tccgcgacca tgccctgtgc ctgccgcccc
     3541 gcgtgatgcc cgacgtcctc ggccccctac ccgtcgacca tctgggcaat tcgatgaacg
     3601 aattcgagcg ctgcaaccag tggctgctct acagcgccct cgcctgcggc atcgcccgca
     3661 cccgcttcgt ctgcctgtgg aacggcaccg gcggcgacgg acccggcggc acccagcaca
     3721 tggtccagga agtccgtcgg cggaccggca aggtcacctg gctcgataca cgcaagctgt
     3781 ggtgatgccg ccactggctc gatgaccatg ctgcgtccgc tgcccctcct gtccctcgct
     3841 gcgctgttgc tcttcgtggg cgtactgtgg tcggaccagc ccctggtcga agccgggcac
     3901 agcatggtcg aactgcagtt cgcgggatcg gccgagcggg ccgcccggat tctcgacacc
     3961 tggcggaccg cgggcgttga ggacgcgatc cgcctcaacc agcagatcga cttcgccttc
     4021 ctgctgacct acccggcggc cctgtccctc gcctgccgca gcctcgccgg acatctcggt
     4081 cccggccagc gcatcggcca cttcctgacc cgcgcagtct ttgcctgcac ggtgctggac
     4141 ggtgctgaga atctcgccat ccaggtcatg ctcgaccacg gcgcccgcga cagcatggcc
     4201 cgccttgcca cagcctgcgc gataccaaag tttgccctcg ccgcctgggc cattggctgg
     4261 tgcctgtggg cgctcttgcg ccgcgtccga ctgccatgag cactcaggcg cacggtcacc
     4321 cgcacggacc acgccagagg cgctctttcg ccgcccgcct gttcgggtac gacctgttca
     4381 tctccttcgc cctcggccat ccgccccgcg gcacccagtc ctatgcctcg gatctggcac
     4441 gccgcctgcg ggagcgggac tttgccgtgt tctacagcga agacgaggcg gcccccggcg
     4501 gcgaactgga ctcgaccctg cgcaacgccc tcgcccgcgc gcgcatcctg gtggtcatcg
     4561 ccaaccgcgg caccctcgcc gacccccgct gggtgcgccg tgaagtggag gagttccgcg
     4621 cactccatcc cggccgcccc atcatcccga tcagcctcga tggcgccctg caggatccgc
     4681 aactggccgc tgcctgcaag gactggctgg acatgagcga caccatctgg ctcgacgaca
     4741 ccgcagaggc cgccgagcac ggactggtga gcccggccgt catcgaccgg cttgtcctcg
     4801 cgcccaccca gatgcgctcg tcccagggct ggcgcatcac ggtcgccgcc gtcagcctga
     4861 cgctgatcgg actcaccgca tcgtccatct ggtccgaaca tcgcgcccgc accaatgaaa
     4921 actccgcccg ccaggaagcc gagcacgcac gccgcgccga acgccaggcg cgcgaagagc
     4981 tgcgcgtctc gacgggcctg cgcctgcgcg ccgaatcatc cgccatgctc ggcggcctac
     5041 gcccggagcc agacgaacgg gccctcctgc aactgctcgc cgcccttcat ctgggacgcg
     5101 gcgacgacac cctcgacccg gatgcggaag gcgagctcct cgggggcctg cgccacggtc
     5161 gcaacctcgt ccgcatgtcg cccgccgccc accccatcct gagccttgct ctcagtcgcg
     5221 acgggaccca cctcgtcacc ggcggcgaag atgccacctt gcagctgtgg aacgcgctga
     5281 ccggcgaccc cgtcggccca ccgctgcgcg cccacgaaga cgccgtgcgg gccgtggatt
     5341 tcagcccgga cgggcagtgg ctggtcagtg ccagcaccga caacaccctg cgtctgtggc
     5401 aggtgggtcg cgacggcctg cagccggctc cgcttccgcc cctgaaaggc cacaccgact
     5461 ccgtgcggac cgtggccttc agcccggacg gtcgcctcat tgcctcgggc agcgacgaca
     5521 acaccgtcat cctgtgggac gcccgcagcg gcacgcccct gccccgacgc ctgaccggcc
     5581 acggcgattc cgtcaccggc ctcgcgttcc tgccggacgg gcggcggctc gtcaccgcca
     5641 gcaacgacat gagcctgcgc ctgtgggaca ttcccagcgg ccgccccctg ggcccgcccc
     5701 tgtccggcca cacggactcc gtcaaatgcg tggccgtctc cccggacggg cgacgcatcg
     5761 tgtccggcgc ctgggacaac acgctccggc tgtgggatcc ggtgacccgc cgccccatcg
     5821 gccgccccct caccggccac cgtgccctgg ttcgcagcgt ggccttcagc gccgacggtc
     5881 ggcgcatcgt gtcgagcagc gacgacggca ccctgcgcat gtgggacgcc cgccgcggaa
     5941 cgctcctcgc gccgcccttg cggggccacc agggcgaagt gaccagcgcc ctgttcgccc
     6001 gcaaggggca gcggatcatc tccggcggca tggaccggag tctgcgccag tgggatccgc
     6061 gcaccacctc gcccctcggc gaagcacagt ctgccggatc cgccgcgctc ctcagcgtgg
     6121 ccgtcagccc ggacggcaaa cgcctggctg gcagcggcga agacctccgc ggccgcctgt
     6181 ggcgggtcgc ctctgcacgc accgcggcaa acgcccgcgg gatcgccctg tcaggcccct
     6241 ccgccgcgtt ctatgccctc gagttcaacg ccgacggcgc ccgcctggcg gggggcgacg
     6301 ccgccggcca gctgtggcaa tgggacggac gcagcggcga aaccctcgcc gcaccccacc
     6361 ccgcccacgc gcgccccatc tccgcgctcg cctacagccc ggacagccgc cgcatcgcca
     6421 ccggtagctg ggacaacacg atccgcatct gggacgccac cagcggcgcc ccgctcgggc
     6481 cgcccctcac cggccatcgc ggctggatcg ccgcccttgc cttcagtccg gacggccgga
     6541 ccctggtctc cgcctcggcc gacacgaccc tgcgccgctg ggacgtggcc accggccatc
     6601 tcctcggggc gcccctgtcc ggccactcca actgggtgct ggcggtggct ttcagcccgg
     6661 acggccggaa cctcgcctcg gcgggcgcgg accgtcagat ccgccgctgg aacgccgtca
     6721 ccggcgcagg catcggcacc ccgtccggcg gccacgaatc ctggatcacc agcctcgcct
     6781 tcagtccgga cagccgctat ctcgtctccg caggctggga tcgcagtctg cgtctgtggc
     6841 gcactgacag cggactcccc gtgggcatcc cgctgcaagg gcaccgggac tggatccaga
     6901 aggtcgcatt ccgcgtagac ggattgcgca tccactctgc cgaccgggcc ggcgccctgc
     6961 acctctgggc cgcaccacgc cactggccgg agatgctgtg cgccaaactc agccgcaacc
     7021 tgagccgcgc ccagtggaag gcctgggtct cgccggacat cgactacgtg gtccagtgtc
     7081 ccggcctgcc cgtgccgccc gactgacgcg tccccctgga gaccgccatg cacgaacccg
     7141 ccgacatcca ccgcctgttc gccgaacgct tcgccgcggc cgatctggac ggcctcctcg
     7201 aactctacgc gccagacgcg ctgctgctgc cccaggcggg cagcgaggtg cgcggccacg
     7261 ccgccatccg cgaagccctc ggcggcttca tcgccctcgg cggacgcttc acgatggcgc
     7321 cgccccgctg cctgcgggcg ggcgatatcg ccgtcctgca cgccgactgg cagctctccg
     7381 ccagcggccc ggacggacag cccgtccacc tggggggaca caccgccgac gtcgtgcagc
     7441 gtcaggtcga cggcacctgg cgcctcatca tcgacaatcc atggggcgac gcggtgaccg
     7501 cctgaagccg gcgccccggc cggccggtgt aaaggagacc ggatatcgca tgcggatttg
     7561 cgaaacggcc ggcgcctgca tatagtcctt gaagaacccc gcacccgcgc ccttcaggag
     7621 accgtcatga cccagaccct tgccgaccgg atccgcgccc ccggtccgaa gaaaatcctg
     7681 tcctgtgacg gcgggggcat cctcggcctg atcagcgtcg agatcctcgc caaactcgaa
     7741 gccgatctgc gcgcacgcac cggcaaaccc gatcttctgc tgggccaatg gttcgatttc
     7801 gtttgcggca ccagcaccgg cgccatcatc gccgcctgca tcgccgccgg catgagcatg
     7861 gagcgcatcc ggacgttcta catcgaaagc ggccagcaga tgttcgacaa ggccagcctc
     7921 ttccgccgcc tgcaatacag ctacaacgac gagcccctcg cccgcaagct gcgcgacgaa
     7981 ttcacccgcg ccctgggcga cgacaccacc ctgggcagcc cgggtctgcg tccgctgctc
     8041 atgatgatga tgcgcaacgc caccaccgat tcgccgtggc cggtctccaa caaccccttc
     8101 ggcaagtaca accagcccgc ccgcaaggac tgcaacctgc gcttgcccct gtggcagctc
     8161 gtgcgcgcca gcaccgcggc ccccacctat ttcccgccgg aggtcgtcac gctcggccag
     8221 ggggatgacg cctacagctt catctttgtc gatggcggtg tcaccaccta caacaacgcc
     8281 gccttcatgg ccttccagat ggccaccgcc acccccttcg gcatccgctg ggccacgggc
     8341 acccgcgaga tgctgatcgt ctcggtgggc accggcagcg tcgccgccgc ccggccggac
     8401 ctcgaggccg acgacctgac cctcatcgaa aacgccaaga ccctgcccgg tgccctcatg
     8461 aacgcggccg ccgcgggatg ggaccgggcc tgccggctga tcggcgagtg ccgcttcggc
     8521 gccctgatcg acagtgaatt cggcgatatg gtcgtcccgc ccggcggcgc caacacctac
     8581 agcggcagca agcagttcgc ctacgtgcgc tacgatccgg acgtcagcca ggccgggctc
     8641 aatgcgctcg gcctctccgg catcaaggcc gcggacgtgc agaccctcga ctccgtggcc
     8701 cacattcccg acatccggac ggtcggcaag acctacgccg atcgcaacgt gaagctggag
     8761 gaacacctgc gcggctttgt ctgagggcct gatccggcaa cttccgccga cagaccggca
     8821 acatttgccg cattcacgcc accttgcgcc gaccgggacg ccgccatgac gcccaaaacc
     8881 tgcttcgtca tccagggctt cggcaagaag accgactaca ccgacggccg cgtgctggac
     8941 atggacgcct cctatggcgt catcaaggac gcggtcgagg ccgccggcct cgtctgtctg
     9001 cgggccgacg agattcccca ttccggtacc atcgacgagc ccatgtaccg gcagctcctg
     9061 caggccgatc tggtgatcgc cgacctgtcc acctacaacg tcaatgcagc cttcgagctg
     9121 ggcgtgcgct acggcctgcg gccccgcgcc accctcatcg ttgccgaaga gggcttcaag
     9181 aaccccttcg acgtgagcca tctggtgatc cgccgctaca agcacctggg cgaggacatc
     9241 ggcgtcaagg aggcccgccg cttcgccgac gagctcaagc tggccatcgc tgccatcctg
     9301 tctgcagacc aggcggacag cccggtctac accttcctgc ccggcctgca gccccctgcg
     9361 ctgccacccc cggccccacg cgccgcccct gtccaggccc aggccgaagc ccccacccgc
     9421 gccggcggct tcgatctcag caaggcgcgc gactggctcg acgggctctt ccacacctcg
     9481 gcccccgagc cgggcacgga tgccgccgcg gacaccgcgg gaccgcccac cctcagccgc
     9541 tgggaacccc cggaagtacc cgctccggcc gccccccgca gtgccaaatc cctgctcgac
     9601 gaagccctcg ccgccctgca acccggggag gatacgccgc ccgatttcat ccgcgcccgc
     9661 aggctgctgg aagccgtccg cgaacagcgc cccaacgacc ccttcgtaat ccagcaactg
     9721 gtccgggcga tttccggcgc cgcccgtcca acgccccagg cagccctgcg ggaggccctc
     9781 gcgctgatga acgagctcgg ccccgccacc accaacgacc cggagaccct cagcctgtgg
     9841 ggcagcgttc accagcgccg atgggaactt gatgcgcagc cggccgatct ggaggaagcc
     9901 atcgccggct tcgagcgagg gtattttgtg aaacaggact ggcaaacagg cagtgcgctg
     9961 gcctttctgc tcgaccgccg cgcacttgag catctgaagt ccggcgcaag cgacgaagcg
    10021 gttgcggacg gcgtgcaggc acgccgcctt cggcgcgagg tgctgcgcct tgccgggcca
    10081 cacatcgacc accccgccga cgatgccgca aagcggttca gcgtgatgtg cgccgtctgg
    10141 gaagcccacc ttgggctggg cgcaaggtcc gaggccgaca tgctcgacgc gcgccttgac
    10201 gctctgccgg taccggcacc cctcaacgcc cgccgaaagg cgcatgggcg caccctggtg
    10261 gcacaactcg acgccttcac gcgcttgcgc ggtggcgccg ccggatcgac gtgacgggat
    10321 ccgtgtcccg caagaggccc tgagcgtgcg gcggccgacc ggcaggcgca ctcgccgttt
    10381 cccccgaacg acgcgccccc gccgccgcgc tgccttcaac ccccgtaacc acccatagat
    10441 ccgcacggcg gacctgccga tactgattcg cgcccgatgc cggcatcgca cctacaagcg
    10501 cgtccctcgc gcgagccgtc ggccatgatg ccctgagggc gcgggcgaac agggcgtcag
    10561 ccctgctccg gatcgccacc ccggcgctcc ttgggcgcgc cgctgaagcg gctcggcggc
    10621 acgccgagcg cgcgccggaa catggcggag aacgctgcct gactcgcgta ccctacctcc
    10681 gccgccacac gggccagcgg caccccgcgc gtgatgtcat ccacggcgcg ggccaagcgc
    10741 agctgctgac gccaggcacc gaagctggta tccagctcgg cgcggaacag acgcgccagg
    10801 gtccgttcgc tggccgccac ctcccgcgcc cactcagcca gtccccggtc atcggccggg
    10861 tcggccatca ggcggtcgca caactgcttg aggcgccggt ccttgggcag gggcaggccc
    10921 agggacaagg gtggcgcgac gcggatctcc tccaggacca gttccatcat caaacgatga
    10981 cgccggtcgg tgcgccgctc ggccagggcc tcgatcaggt tgcgcaacag cggcgacaca
    11041 tccaccacag cacagcggtc cagggccagc ggcgccactt cccgggcgat gtagagcgtg
    11101 cgcagatcca cctgaccgat catcaccacg tcgtgctcca tctccggcgg aatccacact
    11161 gcccgaaacg gcggcacgat ccacgcaatg ccggcggccg caatgcgcag cgcgccggac
    11221 accggatagg ccagttgccc ccacgggtgg cgatggggcg gcactgccac gtcgggcggc
    11281 acacgacgct ggttgacggt cactggctcg tcagcggacg gggccaggcg cgggagacaa
    11341 tcgaaggcga cactgacgac ctcagccggc atgatttgtc tcctttgaac aagctaatga
    11401 cggtatatcg aataacggcc actgctcgaa ccgataccat gggcgtcgat gtgcggaaac
    11461 acgccccaga gcctgccgcc cccccgaccc cagtccggag aacagccatg tcaagcgatg
    11521 ccgcgctgtc cgcgccgccc cgccacgacg cctttgtgat cgccctcatc gggtcggtac
    11581 acggtgtctc gcatttcttc catctgctgc tgccacccct tttcccctgg ctgatgcccg
    11641 aattcggcct ggactattcg cgtatcggaa cgaccatgac ggtcttcttc gtggtttccg
    11701 gcgtgggtca ggccctggcc ggtctggcgg tggatcgctt cggtcccttg cgggtgcttg
    11761 cctcgggcat cggatgtttt gtgcttgcgg gtctgaccct ttcccgggca aacggtttcg
    11821 ctgatctgat gatcgtggcg gccctggctg gtctcggcaa cagcgtcttc catccctgcg
    11881 acttcacggt cctcaaccgt aacgtgtcgg tgccccgcct cggccacgcc ttttcggtgc
    11941 acggtctttc cggcaatgtg ggctgggccc tggcgccgct cttcatgacg ggcatcgcca
    12001 cggctgccgg ctggcgcacc gcggccctgg ccgccggcct gctggcgatt ccggcgctgg
    12061 ccctgctcct cgccggccgg cgtcatctcg tggatgccac gccgccggag caccaggacg
    12121 gtcgccgggc gagcaccgct gaaatcctgc gcgtcggccc cgtgtggctg tgctttgcgt
    12181 tcttcttctt tctcacgaca gccttcggcg ccatccaggc cttcggcacg ccgctgatgc
    12241 agcacctcta tgagctgccc ctgggcatcg cagccggtac cgtgtcgtcc tacctgtttg
    12301 ccggcgcggt ggggatcttc gtcggcggct tcctggcccg cagcgtgcag caggaccggg
    12361 tgatcgccct ggccctgagc gccgccgcag ccatggcggt gctgctcgcc agtgcgcagg
    12421 tgccggccgg ggccgtgctg ccggtgatgg cctgcatcgg cttcctgagc ggtctcgccg
    12481 gcccgtcacg ggatctgctg gtgcgccgcg ccgccaccgc ccgtttcggt caggcagcct
    12541 tcgggcgggt gtatggcttt gtctactccg gactcgacac aggcctcgcc ctggcaccgc
    12601 tggtatttgg cggcttcatg gacagcggcc actacggcac cgtgctggtg ggcatcgccc
    12661 tgctccaggt ggcggcggta ttcaccgcac tcaaggtggg gcgtggctga tggcgcggat
    12721 cttcagcttt ccgccggtgg ctgcgccgga tgcgcgggtg ctgatcctgg gcagcatgcc
    12781 gggcgaagcg tccctgaagg cccgccagta ttacgcccat ccacgcaacc tgttctggcc
    12841 catcctcgga gaactggtgg gcgcccgacc ggatctgccc tacccggaca ggctcgccgc
    12901 gctgcgtcag gcccacatcg ccctgtggga cgtactccag tcctgcgagc gggatggcag
    12961 tctggattcg gccatcgacc cgcagacgcg cgtggtgaac gacttcggtg atttcttcac
    13021 ccgtcacccg cacatcggct ggatcttctt taacggccgg gaagcggaga agaccttccg
    13081 caagcacgcc atccacgtct gcgacgcgga gcgcttccgt tacaccctcc tgccctccac
    13141 aagcccggcc aacgcgtcga ttccgccggc cgtgcgcctg gacgcctggc gcgtcatcgt
    13201 ggatgcgccc aggcgggtgt cggcgccgca tcccggctcg atggccggcg cgggtgcgac
    13261 acttcagggt tcaagcacgt aggtccccgg tgcatcggcg agggccggat gggcctcgtc
    13321 ggccaccggg cgggcgtcca ccagatcgcc gcactggggc ttgagccacg ccatccagtc
    13381 tgtccaccag ctgccgggat gctcggccgc cttggccagc cagccatctg ctgcgtcgct
    13441 gcgccgcgcc gggcccacgt ggtaacgacg cttgggcgga ttgaccggcg gattgacgat
    13501 gcccaggatg tgcccggaac tggacagcac ataacgcttg ggtcccgcca cgtagttcat
    13561 ggtgcggaag gtctgctgcc acggtgcgat gtggtcgtct tcggcagcga ctgcgtagac
    13621 cggctgtgcg atcgcaccca ggtcgatggg ttcgcccgcc accgtgagcg cgtccttctc
    13681 gatcagccgg ttgtgcaggt agagttcgcg cagataccag gcgtgcattg catagggcat
    13741 gcgcgtggaa tccatgttcc agtacagcac atcgaagggc gccggcttct ctccgtacag
    13801 ccagccatgc accacgtagt gccagatgag gctgttcgac cgcagcagac gaaaggccga
    13861 cgccatctcg gcaccgtcca gatagccctt ctgggccatg gtctgggtga ggtagcggat
    13921 gctgccctca tccacgaaga cctcgatgtc gccgggcttg tggaaatcca ccagggtcgt
    13981 gaagagggtg aagtgcgcca ccgggacgtc ttcggccttg aagtgacgat tcgcccaggc
    14041 catgtaggtg gtcagcacgg tgccgccgat gcagtagccg accgcatgaa ccttgtccgc
    14101 accgctcatg gcacgggcgg tctccaccag catgtggaca ccttcggtca ggtaatcgtc
    14161 gaagcgcacg tcgcgcatgt cctcacccgg gttcttccag ctcgtgatga acacgtccag
    14221 tccctggtcg aggagatagc ggatcatgct ttttcgggac gtgagatcaa ggatgtagaa
    14281 cttgttgatc cacggcgtga cgatcaccac cggacgtgcg tggatcttcg gctggctcgg
    14341 ggtgtagtgg atcacctcca gcaggcagtt gcggaatacc accttgccgg gtgtcgtggc
    14401 caggttgacg cccacctgga agtcattggg atcggtcatg cggacgttgc ccgcctccat
    14461 atcggccaga aagttctgga agcccttcac cagactctca ccacgggtct ccatcgccag
    14521 acgcaaggca accgggttgg tcatcaggaa gttggtgggc gccatggcgt tgagccactt
    14581 ccgccaccag aacgcagcgc gccgccgctc cttctgggac agccccggcg tgtcgtagag
    14641 catgtcctgc atgtggtgcg tgaacaccag ataccattcc ttgatcaggt cccaggtcgg
    14701 cgactccccc cacaccggat ctgcaaatcg cgtgtcatca ccatggggct tctccgggtc
    14761 ctcgctgggc aggccaaaga cacgacgcat cgagtgccac tgcagtgccc acaggctggt
    14821 ggacaccttg gcgagtgcat ctgccagctc ctggggatga gccatccacg ccaactgcgc
    14881 atggacgatg ggcgcggtca tgccgatggg gtcgatcgac gcttcaacgc tgtcatgaat
    14941 cttcttcagg gacttgcggg ccgtactgaa cggagcgggg ggttcacgcg atgacatgtt
    15001 caaagactcc tggcgacgta tggtgttgac gcacccacag ggcaaatctt gatcctgccg
    15061 gcgaccgggg cgttgatgta cgtcaagccg gtggcgcgca cgccctgcgc acgccgaccc
    15121 tgcccgtata atgaaaacgt caggttacac cgatgggagt gacgccatgt tcaaacacat
    15181 tcttgttcca accgacggct ctgaactgtc ctcggccacg gtgacgcggg ccgtgacctt
    15241 cgcccgggaa gctggtgcga agatcacctt tttctatgcc cagccggatt tcccgatgcc
    15301 catctacggc gaaggtgcgc tgatcgaccc taccaccccg gaacagttca gcaaggcggc
    15361 agccgctgaa gccgacgcca tactcgcaaa gtcccgtgag ctcgctacgg tggcaggtgt
    15421 cgagtgcgcc ggagtcacgg tcgtcagcga agtaccgtac gaagcaatca tcgaggcggc
    15481 cgaaaaaagc gcttgcgacc tcatcttcat ggcctcccac ggtcggcgag gcttgtcggg
    15541 ttttctgctg ggcagcgaga cacagaaagt gctgacccac agcaagatcc cggtcttggt
    15601 ctatcgctga accggcgccc ccggtgccgc ggtcggcgcg cgtccgtgca ccgggcccga
    15661 cctcgttttg aaagccttca ccacagcggc aataccgctc ggtcaagggc tttatccaga
    15721 aaccggtgac gtaaagggtg gagcgtgctc tgccgagagg ggctttggcg cccctgcatt
    15781 ttttcgacgc ctacaaactg catccgggta cgccgtcgta acaggtggac atgtcgcttg
    15841 cgcacggtca tagcgacaga ccagaccttg tcgtgctgca gaaggtacag cgcgcgcatg
    15901 aagctcgggg ctaccgcgtt ggccatcgcc aagcacatca ggttgtaagt gcgacgctga
    15961 cagccttcat cgctttaatc agctgagtaa aaatgaaaat gccgttcagt gagcactgaa
    16021 cggcaatacc gcttaggcgg gagtttattt ttagcgcata gcggaacagt ccggattccg
    16081 atacgccagg cggtttgtct aaacgccgtc gagtttgttg atttctgccg acgtgcgctg
    16141 gaaaaacagg gtcatcaggc tggacgcagc acagacaagc cagaagccga aaaaaccgat
    16201 gctataggtc gccgtcgccg aaaagtgcac cggctcaccg aacaggtaca gctcctgtgg
    16261 attgacgacc gtaaagaata cgatctcggc gagaccggcc accagaaagg acggccacag
    16321 gacttcgatc agctttctca tctttcatgc tcctccatgc cgagcgctct gctgaatgga
    16381 ctgctcagcg gacagtcggc ttaacccccg cgtgcgcggc gctcatcttc agccatatgt
    16441 gatttggcat aacgatacag gtagccgccc atcacgatga cgaacccgat ggtgaacaag
    16501 ctcaacatgc caatatcggt gctgaacaat tctttccaag ccatgatttt cctccatgtc
    16561 aatcgacggg tttgaggtcc gccgaatcca ggcggatctc cgtctctgtc gggaggaacg
    16621 cggtccctgt cagcctccag ctgcgggact catcttctaa aagtactttc cagtgggcgc
    16681 tcacgagttc ctgggcgagc gcgccccggt atgcccccgc ctgcccctgc aggttcaggt
    16741 gttggtccag gcctccacgc gtcgggtggg caatcgtcac cagaacccgc tcgggaaggg
    16801 caatgccggg ctttgccgtc agcgcaatcg ccacaccgtc ggccgagaac ctcacctgcg
    16861 cgacaagccc cagatcgcga gccaccaccg cacgcccgat ggtctggttg atcgccagac
    16921 cctgcttgta gtaatcgtca gccaccaatc catcaaaaga cgtgattgcc agccacaggg
    16981 tgatgaaccc agcgacgatc gccgtcgctg gcatcgccat caacaaccat ggccaacgct
    17041 cccgatacca gggcttgtca tcgcgaatcg tattcagggt ggaagtattc attgcggtag
    17101 caggaatgca gtcttttcac gaacggcgac ggtttggtcg tccgccgctt tcacttcgat
    17161 gtagatggta ttggagccag gcttaccgga ctccggcggc gcagcaacct cgaccgtcac
    17221 ggccttcaga ccggcggcgt cgacttcgaa gggcttgttt ggcgaaatcc ggatctgatc
    17281 cagaccgctc acggacaagg taaaggtacg gggcttttcg gtcatattca tgacctgcag
    17341 gttgtagacg ttagcgatca gaccaccctc cacctctcgg ctgagggtcg agcggtcctt
    17401 gatcacgtcc acccgcagcg tggagcgggt cgccagaccc cagaccagtg caatcaccag
    17461 cgccaccagg accgacgaat agatgaggat gcgcggtcgc atcacgtggc cgaggatttc
    17521 cttgcgaccc caatgacgcc gcatggcgtt ctcggtggtg taacggatga gccccttcgg
    17581 g
//
LOCUS       DAOQPA010000078        17374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1645_length_17374_cov_21.629251, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000078 DAOQPA010000000
VERSION     DAOQPA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1645_length_17374_cov_21.629251"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1498)
                     /gene="dnaB"
                     /locus_tag="PKX01_11375"
     CDS             complement(107..1498)
                     /gene="dnaB"
                     /locus_tag="PKX01_11375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMZ76773.1"
                     /translation="MRNASLSEDPALASLRLPPHSLEAEQSLLGGLLIDNTVWDRVGD
                     IVSEVDFYRDDHRRIFRQIAKLIELGKPADVVTVYEALEKAGEAEHVGGLAYLGEIAN
                     STPSAANVRRYGEIVRERAILRKLVAVGDQIAASALAPSGKDAKTLLDEAEAKVFEIA
                     EAGARTTTGFVPIQPLLGQVVDRIQELYDRDSPSGITGVATGLTDLDEKTSGLQPSDM
                     IVVAGRPGMGKTSFALNIAENVAVECGLPVAVFSMEMPGTQLATRFLSSVGRLDQHRV
                     RTGKLTDEEWQRLTFALGKLHEAPIYIDETPGLNPTDLRARCRRLHRQCGRLGLIVID
                     YLQLMTSLKESDNRAAELSEISRSVKSLAKELHVPIIALSQLNRSLEQRPNKRPVMSD
                     LRESGAIEQDADIIMFIYRDEIYNPDSQDKGTAELIVAKHRNGSTGTIRMTFIGEYTR
                     FENYVGGAAVAEY"
     gene            complement(1607..2056)
                     /gene="rplI"
                     /locus_tag="PKX01_11380"
     CDS             complement(1607..2056)
                     /gene="rplI"
                     /locus_tag="PKX01_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMZ76774.1"
                     /translation="MQIILLDKVVNLGGLGDVVKVKDGYARNYLIPQGKAKRATATNL
                     AEFEARRAELERQAAEQFAAAQAEGAKLEGQSVTIARKAGVDGRLFGSVTNADIADAL
                     KAQGFAVEKSAVRMPEGPLKTVCEAQIVVSLHTDVTATITVTVVGEN"
     gene            complement(2072..2341)
                     /gene="rpsR"
                     /locus_tag="PKX01_11385"
     CDS             complement(2072..2341)
                     /gene="rpsR"
                     /locus_tag="PKX01_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMZ76775.1"
                     /translation="MAFKPAMKRKDDRGGRGLFKRRKFCRFSAEKIEEIDYKDVDILK
                     DFITENAKIMPARITGTKSGYQRQLSTAIKRARFLALLPYTDLHQ"
     gene            complement(2307..2657)
                     /gene="priB"
                     /locus_tag="PKX01_11390"
     CDS             complement(2307..2657)
                     /gene="priB"
                     /locus_tag="PKX01_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HMZ76776.1"
                     /translation="MQEAGRNSLELEGVLTEIGALRRTPAGIPVLACRIEHRSRQMEA
                     GRLRDVGVELQAVVVGDKTSLLAAAPLGSLLHVTGFLAAKSLRSRAPILHIDTIEFLE
                     GNRHGIQARNEAQG"
     gene            complement(2660..3040)
                     /gene="rpsF"
                     /locus_tag="PKX01_11395"
     CDS             complement(2660..3040)
                     /gene="rpsF"
                     /locus_tag="PKX01_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMZ76777.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYTTLVTSQGGQVHRLEDWGR
                     RQLAYPIQKIHKAHYVLMNIECDQPTIAELEHGFKFNDAVLRHLSIRMKRAVTAPSPM
                     MKEEKARSLTPAADEAKPAEQPAA"
     gene            complement(3177..3267)
                     /locus_tag="PKX01_11400"
     tRNA            complement(3177..3267)
                     /locus_tag="PKX01_11400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3231..3233),aa:Ser,seq:gga)
     gene            complement(3289..5361)
                     /locus_tag="PKX01_11405"
     CDS             complement(3289..5361)
                     /locus_tag="PKX01_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HMZ76778.1"
                     /translation="MKSSHSYFVRLGRYYGWYSAGFFVLIGLLAIAEQLGMTPRWIGQ
                     IFLLATIALYAAIGVMSRTSDVSEYYVAGRRVPAVFNGMATAADWMSAASFIGLAGTL
                     YYTGFEGLAFVTGWTGGYVLVALLLAPYLRKFGQFTVPDFLGARYGGHAARIVGVVAA
                     VLASFVYVVAQIYGVGLITSRFIGIEFEIGVFVGLAGILVCSFLGGMRAVTWTQVAQY
                     LILIVAYLVPVTILSYKVTGVPLPQLTYGGVLKDLGNKELELFSEPRENEVRTQYRYR
                     ADDYARKIIDLPDSLAAERTGQLQRVEELRTQGASARELAAAERALRDMPRTPDEARV
                     KWERARQESLVRAAPPVPHAQAHPGPDRAARDSARNNFIALAFVLMVGTAALPHILMR
                     YYTTPSVRQARNSVFWSLFFIFLLYVTAPAYAVFAKWEVYSGLVGTPIASLPAWVAAW
                     GRVGLVKIDDINGDGILQLAELSLNSDAILLLVPEIAGLPYVVSGLVAAGGLAAALST
                     ADGLLLTISHALSHDLYYKMINPEASTQRRLIITKSQLLIVAVCAAWVASLRPDGILF
                     MVGLAFSIAASAFFPALVLGIFWKRANRWGAVSGMAAGLGITLYYIVRTHPFFGGSLS
                     EAWFDIHPISCGIFGVPVGFVTIVVVSLLTPPPDAATQRLVDFIRMPDGIEDFPDRDE
                     KVFDAGGS"
     gene            complement(5372..5638)
                     /locus_tag="PKX01_11410"
     CDS             complement(5372..5638)
                     /locus_tag="PKX01_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HMZ76779.1"
                     /translation="MAVDSAGRRAYWRANLALTAVLLVIWAAVSFVPGWYAEDLEAYS
                     VFGWPLDFYMAAQGSLIVFLVIVWLYDRCMRRLERRLGVPDERD"
     gene            complement(5694..7127)
                     /locus_tag="PKX01_11415"
     CDS             complement(5694..7127)
                     /locus_tag="PKX01_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HMZ76780.1"
                     /translation="MSSSRESRPLSGDGQALASLLRRAPVSVPPESPVRDVLKRMLAA
                     RVGSVVVADVDTGFPLGVLTHRDVLERVVLAAGDLDVPVASVMTGGVMSLPLGANVHL
                     ARLLLAQRRLRRVVVVDADGRLAGVVSRGDLYAQRQPCADDLVSRIRAADHVEALRDA
                     ARGVRDAAARWLDDGAGAGPVAEWIALLNDLVAQAALDLALRDVELPLVRWCWLAFGS
                     EGRLEQTFETDQDNGLIFEVPDDVDADAMRERFLPWAQRVNDVLDTCGFARCAGGIMA
                     GNPTWCLSVGEWRERFLSWMLSADPEALLNATIFFDFRPLAGDWELAGRLQEWLLART
                     AESELFLRHMAGNALRGGPPLGRIRDFAVDRKTGRLDLKRDGSRPFVDVARIYALALG
                     LRETATADRLRGAGRLLNWSRREIDAVVEAFHCIQRLRLDRQQRGQGPANLIAPEELN
                     ELERATLKEAFRQARKLQARLQIRYQL"
     gene            complement(7197..9164)
                     /locus_tag="PKX01_11420"
     CDS             complement(7197..9164)
                     /locus_tag="PKX01_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMZ76781.1"
                     /translation="MKSFVQAHVAAPALLADEIGLIVGTDPARRLKAQADPDLNSIAD
                     AVNALADQRRSLQDDVEARIREARLSVEEERNRLAALMSELTQSVVVCNLDGRVLLYN
                     NRARLQFKALSDAPAAAGGGEIIGLGRSIYAVFERNLIAHALESIQERLRRQSAQPVA
                     NFVTTTRAGQLLRVQMAPVLSVGEGSAEPAVTGFVLMLDNITRNFESETRRDQVLHTL
                     TEGSRSALANVRAAVEMLEFPDLDADLRERFLTVIRDEVRGMSERLDKTASEFADSLK
                     TRWPLDEMLGADLVAAAQRRIEKKIGLPTKLEDVDDSVWVKVDSFSLLQAVGYLASRL
                     SDEFEVREVRLRLTSAGRLVHLDLIWSGQAMSTETVMAWELEPMSFAGESSPLTVRDV
                     IERHDGEIWLQREKTRHRAFFRVLIPSAAPQEQVESSAYLKGDSRPEYYDFDLFKHSE
                     ASDALDDRLLSELTFTVFDTETTGLNPSEGDEIIQIGATRIVNGKLLKSESFEQLVDP
                     LRDLPEASTKIHGITPEMLEGQPALDKVLPAFHAFSQDTVLVAHNAAFDMRFLQIKEE
                     SLGVRFTQPVLDTLLLSAVIHPAQESHRLEAICERMGVNVMGRHTAMGDAIVTGEVFL
                     KMIPLLAEAGIRTLGDARRASEKTYYARVKY"
     gene            complement(9359..9733)
                     /locus_tag="PKX01_11425"
     CDS             complement(9359..9733)
                     /locus_tag="PKX01_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76782.1"
                     /translation="MTKKILIADDEQNIVISLEFLMKREGFAVCIANDGQEALDKVRS
                     ESPDLVLLDVMMPRKNGFEVCQEIRADGALQGVRILMLTAKGRETEVSKGLAMGADAY
                     MTKPFSTKELVARVKSLLELPA"
     gene            complement(9814..12570)
                     /locus_tag="PKX01_11430"
     CDS             complement(9814..12570)
                     /locus_tag="PKX01_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ76783.1"
                     /translation="MLSGTTVVAVSFLYLAALFGVAYYGDKRADAGRSVIANPTIYAL
                     SLAVYCTTWTYYGSVGRAASSGIGFVPVFLGPTLVVGLWWFVMLKMIRIGKSNRITSI
                     ADFIASRYGKSHLLGGLVTVIAVLGVIPYIALQLKAVSGSFSILIHPQGLLTPIRTGA
                     LPWMTDTTFYVALMMAAFTILFGTRHLDATERHEGLVAAVAFESLVKLVIFLVVGGFV
                     TFGLFDGFGDLFSQAAEDPALRALMVVETGGNSYGNWFALTVLSMLAVMFLPRQFQVA
                     VVENVNEKHLRRAIWLFPLYMMLINVFVLPIALGGLIYFRGQAVDADTFVLTLPLASG
                     HPLLALLVFIGGLSAATGMVIVETIALSTMVCNDLVMPALLRFSRGALRGHNDLSGLL
                     LAIRRGAIVLILLLAYLYFRLAGEAHALVSIGLISFAAVAQFAPAMLGGMYWRGGSRA
                     GALAGLSAGFLVWGYTLLLPSFAKSGWLPGDFLQGGLFGLEFLRPQELFGLRGLDPMA
                     HSLFWSLFANVGAYTWVSLLRPPNAVEAGQATLFVDVFRQGRPSGGAFWRGSARVGDL
                     LPLIARFVGLERAQALFSSYARERGVERVADLEADAGLVHFAETTLAGAIGGASARVM
                     VASAVKEEPLGLDEVMHILDEASQVRAYSQRLEQKSRELEAATAELRHANDRLKELDR
                     LKDDFMSSVTHELRTPLTSIRAFSEMLLEDPRIDLADRKRFLGIIVAETERLTRLVNQ
                     VLDMAKIESGHAEWHNGEVNLVEVVEQAVASTSQLFKDRGVSLELDLPASLPVLLADR
                     DRLIQVMLNLLSNAVKFVERDGGQVRVKLAETSDGVQVDVSDNGPGLTLDEQTVVFEK
                     FRQGGNTMTAKPQGTGLGLPISRQIVEHYGGRLWVESAPGEGARFSFFLPCGRSTEAQ
                     ERAG"
     gene            complement(12612..12800)
                     /locus_tag="PKX01_11435"
     CDS             complement(12612..12800)
                     /locus_tag="PKX01_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76784.1"
                     /translation="MKATLMGQRLVAVFVLGCVLANFPVLSIFNDDGAWQGIPLLYLY
                     VFGLWAAVIVLMAWIVER"
     gene            complement(13107..15065)
                     /gene="acs"
                     /locus_tag="PKX01_11440"
     CDS             complement(13107..15065)
                     /gene="acs"
                     /locus_tag="PKX01_11440"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMZ76785.1"
                     /translation="MTSETTQKLYYPSEEVVKNAAVSGMEAYKALCAEAEADYEGYWG
                     KRAKELIEWQTPFTQVLDESNTPFFKWFADGKLNVSYNCLDRQVKNGLGDKVAMIFEG
                     DKGDVTKVTYGDLLTRVSKFANALKSLGVKKGDRVVIYLPMSIEGVVAMQACARIGAT
                     HSIVFGGFSAQSLADRVNDAGAVVLITSDGQNRGGKALPLKAIADEALASGCPSIQKV
                     LVVKRTGGETAMVEGRDVWADDIVAQQSDVCEPEWVEAEHPLFLLYTSGSTGKPKGVQ
                     HSTGGYLLHAILTMKYTFDIKPNDIFWCTADIGWVTGHTYITYGPLACGATEIVFEGV
                     PTYPDAGRFWKMIQDHKVSVFYTAPTAIRSLIKASDTTPAVHPKNYDLTSLRILGSVG
                     EPINPAAWEWYYENVGGSRCPIVDTFWQTETGGHMITPMPGATPLVPGSCTLPFPGIQ
                     AAVVDETGTDVPWGQGGILVVKRPWPSMIRTIWGDPERFVKSYYPADFQGKLYLAGDG
                     AIRDKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVAHEKVAEAAVVGRPDDLTGEA
                     IVAFVVLKGARPSGDEAAKVVKELQNWVGQEIGPIAKPKDIRFGENLPKTRSGKIMRR
                     LLRQLAKGEEITQDTSTLENPHILDQLK"
     gene            15646..17274
                     /gene="mshL"
                     /locus_tag="PKX01_11445"
     CDS             15646..17274
                     /gene="mshL"
                     /locus_tag="PKX01_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HMZ76786.1"
                     /translation="MVHNVRLQDLLFALARDARLNVDIHPGLNGTVTLNAIDQTLPQI
                     LTRLARQADLRFELDGTHLAVMPDSPYLRTYRVDYVNLSRSVTGTVSTNTQIATSGSG
                     SAVSGSTTLQTAALPNGGNVSATRIENASRNQFWESIERNIRDLLRETDKLLPEGSSD
                     TVIENLETTSTSGTGVQPSATAARSRTRTTSASPQAGIAASPNPALLQNSATTVVHKA
                     TFREAASVIVNRETGVLTVRATGRQHEKVQEFLDQVVAAARRQVLIEATILEVGLSDT
                     YRQGIDWNRVLSSGTRFGITGPTLGSSAGNAVNPFSVAYKSADGIDSTIRLLEGFGTV
                     KVLSSPKLSVLNNQTAVIKVVEEYVYFNVKADTTQTTNAGALTTVTTTPQAVSVGLVM
                     TVTPQVSEADEIILNVRPTISSIADFRRDPNPNIPATIPNLVPQIRTREIESVLRIEN
                     GETAVLGGLMEDRVDFKTGRVPGVGAVPLLGEVFTTRDNLVQKTELVIFLRPVVVRDG
                     NLLAASPLRNSLPRDDFFRRGPLPGTRNFPDTTP"
BASE COUNT         3367 a         5923 c         5167 g         2917 t
ORIGIN      
        1 ccgcgccgcc tttgctgcac cgcgtgaaga agaggtgcgc attataaaga actatcgggc
       61 cccgtcaaca cgttagtgaa aaataaattt cacccgcgtg tagcaatcaa tactccgcca
      121 cggcggcgcc gcccacgtag ttctcgaaac gcgtgtactc gccgatgaag gtcatgcgga
      181 tggtgcccgt ggagccgttc cggtgcttgg ccacgatcag ttcagcagtg cccttgtcct
      241 gggagtccgg gttatagatt tcatcccggt agatgaacat gatgatgtcc gcgtcctgct
      301 cgatggcacc ggactcccgc aggtccgaca tgaccggacg cttgttgggg cgctgctcca
      361 ggctccggtt gagctgggac agggcaatga tggggacgtg caactccttt gccagggatt
      421 ttacggagcg ggaaatctcc gacagctctg cggcccggtt gtcggactcc ttgagcgacg
      481 tcatcagctg cagatagtcg atgacgatca gccccaagcg tccgcactgc cggtgcaggc
      541 gtcggcagcg ggcgcgcagg tccgtcgggt tcagccccgg cgtctcgtca atgtagatgg
      601 gcgcctcatg gagcttaccc aaggcgaagg tgagacgctg ccattcctca tcggtgagct
      661 tgcccgtccg cacccgatgc tggtccagac gcccgaccga agacagaaaa cgcgtcgcca
      721 gctgggtacc tggcatttcc atggaaaaca cggccaccgg cagcccacat tcaaccgcca
      781 cgttctctgc gatattgagg gcgaacgagg tcttgcccat accagggcgc cccgcgacga
      841 cgatcatgtc tgagggctgc aggccggagg tcttttcatc cagatcggtc aacccagtgg
      901 cgacgccagt gattccgctc ggggaatccc ggtcgtagag ttcctggatg cgatccacga
      961 cctgcccgag cagcggctgg atgggaacga agccggtcgt ggtccgcgcg ccggcttcgg
     1021 cgatctcgaa gaccttggcc tcagcctcgt cgagcagcgt cttcgcatcc ttgccagatg
     1081 gcgccagcgc actggccgcg atctgatcac cgaccgcaac cagcttgcgc aggatcgcgc
     1141 gctcgcggac gatctcgcca taacggcgca cgttggcagc tgaaggcgtg ctgttggcaa
     1201 tctctcccag atacgcaagc ccgccgacgt gctcggcctc gccggccttt tcgagggcct
     1261 cgtagaccgt caccacgtcc gccggcttgc cgagttcgat gagtttggcg atctgccgga
     1321 agatgcgacg atgatcgtcc cggtagaagt cgacctcact gacgatgtcg cccacccgat
     1381 cccacaccgt attgtcgatg agcagcccgc ccagcaacga ctgctcagcc tcaagcgaat
     1441 ggggtggcag tcgaagcgaa gcgagggcgg gatcttcgga aagggaagcg ttgcgcatgg
     1501 aagtcaccac aataaaccgg ggccgcgacc ctcggccgcg cacccaagaa aaaagggctg
     1561 caccgggcag cccttttcca cagcagcgag ccgaagcccg aaccgatcag ttctcgccga
     1621 ccaccgtcac ggtgatcgtg gccgtcacgt cagtatgcag ggacacgacg atctgggctt
     1681 cacagacggt cttcagcggg ccttccggca tccgcacagc agacttctcg acggcaaagc
     1741 cctgcgcctt cagcgcgtcg gcgatgtcgg cattggtcac ggaaccgaac agacggccat
     1801 ccacacccgc cttgcgggcg atggtgacgg attgaccttc cagcttggcg ccttcggcct
     1861 gggcggcagc gaattgctcg gccgcctggc gctccagttc ggcgcgacgg gcctcgaatt
     1921 cagccaggtt ggtggccgtg gcacgcttgg ccttgccctg ggggatcagg tagttacgcg
     1981 cgtaaccatc cttgaccttg accacatcac caagaccacc gaggttcaca accttgtcca
     2041 gcagaatgat ttgcatgctt gttctcccgg attactggtg cagatcggtg tagggcagca
     2101 gcgccaggaa gcgcgcacgc ttgatcgcgg tggacagctg acgctggtaa ccggacttgg
     2161 tcccggtaat gcgggccggc atgatcttgg cgttttcggt gatgaaatcc ttcaggatgt
     2221 ccacatcctt gtagtcgatt tcctcgatct tctctgcgct gaagcggcag aacttacgac
     2281 gcttgaagag gccgcggcca ccgcggtcat ccttgcgctt cattgcgggc ttgaatgcca
     2341 tgtctgtttc cttccaaaaa ttcgatcgta tcgatatgca gtatcggtgc cctgctacgc
     2401 agacttttcg ccgcgaggaa gccggtgacg tgcagcaggc ttcccagggg cgccgcagcc
     2461 aggagggacg tcttgtcccc taccacgacc gcctgcaatt cgaccccgac atcgcggagc
     2521 cgccctgctt ccatctgcct cgaccggtgt tcgatgcgac atgccagcac ggggatcccc
     2581 gcaggcgtac gacgcagcgc accgatttcg gtcaggacac cttccagctc aagactgttg
     2641 cgccctgctt cctgcaaaat caggcggcgg gctgctcggc cggcttggct tcgtcggccg
     2701 cgggcgtcag ggagcgggcc ttctcttcct tcatcatggg cgacggggca gtcacggcgc
     2761 gcttcatgcg gatggagaga tgacgcagca cggcgtcgtt gaacttgaag ccatgctcca
     2821 gttcggcgat ggtgggctgg tcgcactcga tgttcatcag aacatagtgg gccttgtgga
     2881 tcttctggat cgggtaggcc agctgacgac ggccccagtc ttccagacga tgcacctgac
     2941 caccctgact ggtcacgagc gtcgtgtaac gttcgatcat cgcgggcacc tgctcgctct
     3001 gatcaggatg cacgatgaag acgatttcgt aatgccgcat gggatctcct tatggataat
     3061 agcccgccga ccatgcgggg cggtcgggca aggtgaaaca cccccggatg cggccgccta
     3121 taaacggaaa gcgtgcgcac ttgcatgcgc acgccgcgaa catccgggta aattcttggc
     3181 ggagagggcg ggattcgaac ccgcgtcagg gtattaccct gaacacgctt tccaggcgtg
     3241 cgacttaaac cactcatcca cctctccaag tggaggcggg atcatacatt aacttccgcc
     3301 cgcgtcaaac accttttcgt cgcgatccgg aaaatcctcg atgccgtcgg gcatgcggat
     3361 gaaatccacg aggcgctgcg ttgcggcgtc gggcggcggg gtcagcagac tgaccaccac
     3421 gatggtcacg aatcctaccg gcacgccaaa gatcccgcag gagatcggat gaatatcgaa
     3481 ccaggcctcg gacaggctgc cgccaaagaa cggatgggtg cgcacgatgt aatacaaggt
     3541 gatgccgagc cccgctgcca tgccggagac ggccccccag cgattggcac gcttccagaa
     3601 gatgcccagc accagcgccg gaaagaaggc cgacgcggca atcgaaaagg ccaggcccac
     3661 catgaacagg atgccatccg gtcgcaggga ggcgacccag gccgcgcaca ccgcgacgat
     3721 cagcaattgt gacttggtga tgatgaggcg acgctgcgtc gaggcctcgg gattgatcat
     3781 cttgtaatac aggtcgtggg acagggcgtg ggagatggtc aggagcaggc cgtccgccgt
     3841 ggataacgcc gcagccagcc cgcctgccgc caccagaccg gagaccacgt agggcaggcc
     3901 ggcaatttcc ggcaccagca gcaggatcgc gtcgctgttg agggagagtt cggccagttg
     3961 caggatgccg tcaccgttga tgtcgtcgat cttcaccaga ccgacccggc cccacgccgc
     4021 cacccacgcc ggcaaggacg cgatgggcgt accgacgaga ccgctataca cctcccattt
     4081 ggcgaatacc gcgtaggctg gagcggtcac gtacagcaaa aagatgaaga acagggacca
     4141 gaacacggag ttgcgcgcct gccgcaccga gggcgttgtg tagtagcgca tcaggatgtg
     4201 aggcaatgcg gccgtcccga ccatgagcac aaaagccagc gcgatgaagt tgttgcgggc
     4261 gctgtcgcgc gccgcccggt cgggacctgg gtgggcctgg gcgtgcggca ccggcggagc
     4321 cgcgcggacc agactttcct gacgtgcccg ttcccacttc acccgagcct catccggcgt
     4381 acgcggcatg tcgcgcaagg cccgctccgc tgcggccagt tcgcgggcgg aggcgccctg
     4441 agtacgcagt tcctcgaccc gttgcagctg ccccgtccgc tcggcggcga gggaatcggg
     4501 cagatcgatg atcttccggg cgtagtcatc cgcccggtag cggtactggg tgcggacttc
     4561 attctcccgc ggctcggaga acagctccag ctccttgttg ccgaggtcct tcaggacgcc
     4621 gccgtaggtc agctgtggca ggggaacccc cgtcaccttg taggacagga tggtcaccgg
     4681 caccagatag gcgacgatca gaatgaggta ctgggccacc tgggtccacg tcaccgcccg
     4741 catgcccccc aggaaggagc acaccagaat gccggccagg cccacaaaga caccgatctc
     4801 gaactcgatc ccgatgaagc ggctggtgat gagacctacg ccgtaaatct gcgccaccac
     4861 gtacacaaag ctcgccagca ccgcagccac cacacccaca atgcgtgccg catggccgcc
     4921 atagcgggcg ccgagaaaat ccggcacggt gaattgcccg aacttgcgca ggtacggcgc
     4981 gagcagcagc gccaccagca cgtatccccc cgtccagccg gtgacgaagg ccagtccttc
     5041 gaagccggtg taatacaggg tgccggccag cccgatgaat gacgcggcac tcatccagtc
     5101 cgccgccgta gccatgccat tgaacaccgc aggcacgcgg cgcccggcca cgtaatattc
     5161 ggaaacgtcg gacgtgcggc tcatcacgcc gatcgccgca tagagggcaa tggtggccag
     5221 caggaagatc tgcccgatcc agcgcggggt cattccgagc tgctcggcga tcgcgagcag
     5281 tccgatcagc acgaagaacc ccgccgaata ccagccgtaa taacgaccca ggcgaacgaa
     5341 atagctgtgc gatgacttca tggcagacgc ttcagtcgcg ctcgtcgggc accccgaggc
     5401 gacgctcgag acgacgcatg caccggtcgt acagccacac gatgacgaga aatacgatca
     5461 gtgacccctg ggccgccatg tagaaatcga gcggccagcc gaacacggaa taggcctcca
     5521 gatcttccgc ataccagccc ggcacaaaac tgaccgcggc ccagatcaca agcaatacgg
     5581 cagtcaaagc cagattggcc cgccagtagg cgcgacgccc tgcactgtct accgccacgc
     5641 acattcctcc ttggcccgat cggcactggg cagagtatag ccgggctctc gacttacaac
     5701 tgatagcgga tctgaagccg cgcctgcagc ttgcgggcct gacggaaggc ctccttgagc
     5761 gtggcccgct ccagctcgtt caactcttcg ggtgcaatca ggtttgccgg tccctggccg
     5821 cgctgctgcc gatccagacg cagtcgctgg atgcagtgga aggcttccac gacggcatcg
     5881 atctcgcgac gggaccagtt cagcaagcgc cccgcgcccc gcagtcggtc ggccgtggcc
     5941 gtctcccgca accccagggc cagggcatag atgcgcgcca cgtccacaaa gggccggctg
     6001 ccgtcgcgct tgagatccag ccggccggtc ttccgatcca ccgcgaagtc gcggatccgc
     6061 ccgaggggcg gaccgccccg cagcgcattc cccgccatgt gccgcaggaa cagctcgctc
     6121 tccgccgtcc gcgccagaag ccattcctgc aagcgtccgg ccaactccca atcccctgcc
     6181 agcgggcgaa agtcgaaaaa gatggttgca ttcagcagcg cctcggggtc cgccgacaac
     6241 atccagctca ggaagcgctc ccgccattcc cccaccgaca gacaccaggt cgggttgccg
     6301 gccatgatcc cccccgcaca gcgggcgaat ccgcaagtgt cgagcacatc attgacccgt
     6361 tgcgcccagg gcaggaaacg ctcccgcatg gcatcagcat ccacatcgtc cggcacttcg
     6421 aagatcaggc cgttgtcctg atccgtctcg aaggtctgtt ccagacggcc ttctgagccg
     6481 aacgccagcc agcaccatcg caccagcggc agttcgacat cccgtagagc caggtccagg
     6541 gccgcctgcg ccacgagatc attgagaagg gcaatccatt cggcaaccgg accggccccg
     6601 gcgccgtcat ccagccatct ggccgctgca tcgcggacac cacgggcagc gtcgcgcagc
     6661 gcctcgacat ggtcggcagc ccggatccgg ctcaccagat cgtcggcgca cggctgccgc
     6721 tgggcataca gatccccgcg cgacaccact ccggccagac ggccgtccgc atcgaccacc
     6781 acgacccggc gcagacgacg ctgcgccagc agcagacgag ccaggtggac attcgcaccg
     6841 agcggcagac tcattacgcc gccggtcatc acgctggcaa ccggcacgtc cagatcgcct
     6901 gctgcgagca ccacccgctc cagcacatcg cgatgcgtca gaacgcccag cgggaagccc
     6961 gtgtccacgt cggccaccac caccgaaccg acgcgggcgg ccagcatccg cttgagcacg
     7021 tcacgcacag gcgattcggg cggcaccgac acaggagcgc gccgcagcag ggacgcaagc
     7081 gcctgaccat cgccagataa aggacgggat tcccgcgacg aactcatgcg cccctcctgg
     7141 ccggcggcaa catcaccgga ttgcggtcgg agagcacctg cgccctcatc aaccgctcag
     7201 tacttcaccc gtgcgtaata ggtcttctcc gacgcacggc gagcgtcgcc cagcgtccgg
     7261 atgcccgcct cggccagcag cgggatcatc ttcaggaata cctctcccgt gacgatggca
     7321 tcccccatgg ccgtgtgacg ccccatcaca ttgaccccca tgcgctcgca gatggcctcc
     7381 agacggtggg actcctgggc cgggtggatc accgccgaca acagcagcgt atccagcacc
     7441 ggctgggtga agcgcacgcc caggctctct tccttgatct gcaggaaacg catgtcgaac
     7501 gccgcgttgt gggccaccag caccgtatcc tgggaaaacg cgtggaatgc cggcaatacc
     7561 ttgtcgaggg cgggctgccc ttcgagcatc tcgggcgtga tgccgtggat cttggtggag
     7621 gcctccggca ggtcgcgcaa gggatccacc agttgctcga aggattcgga cttgagcagc
     7681 ttgccattga cgatgcgggt ggcaccgatc tggatgatct catcgccctc ggaggggttg
     7741 agaccggtcg tttccgtatc gaagactgta aaggtcagct cggagagcag gcggtcgtcg
     7801 agggcatcgc tcgcttcgga atgtttgaag aggtcgaaat cgtaatattc cggccggctg
     7861 tcacctttca gataggcgga cgactccacc tgctcctggg gcgcggccga cgggatcagc
     7921 acgcggaaga aggcgcgatg acgcgtcttt tcccgttgca gccagatctc gccatcatga
     7981 cgttcgatga catcgcgcac cgtgagcggc gaactctcgc cggcaaagct catgggctcg
     8041 agttcccagg ccatcaccgt ctccgtgctc atggcctgac cggaccagat cagatccaga
     8101 tgcaccagcc gcccggccga ggtcaggcgc agacgcacct cgcgcacctc gaactcgtcg
     8161 gacagacggc ttgccaggta gccaacggcc tgcagcaggg agaagctgtc caccttgacc
     8221 cacacgctgt cgtccacatc ttccagcttg gtcggcaggc cgatcttttt ctcgatgcgg
     8281 cgttgagctg ccgccaccag gtctgcgccc agcatttcat ccagcggcca gcgggtcttg
     8341 agggagtccg caaactcgct ggcggtcttg tccagacgtt cactcatccc ccgcacttcg
     8401 tcgcggatga ccgtcaggaa acgttcgcgc agatcggcat cgagatcggg aaattccagc
     8461 atctccacgg cagcgcgcac gttggcgagc gccgagcgac tgccttcggt caaggtgtgc
     8521 aggacctgat cccgtcgcgt ctcggactcg aaattccggg tgatgttgtc gagcatcagc
     8581 acaaagcccg tcaccgccgg ctccgcgctg ccctcaccca ccgacagcac cggcgccatc
     8641 tgcacgcgca agagctgccc ggcgcgggtc gtcgtgacga agttggccac cggctgggcg
     8701 ctctgacgcc gcagacgttc ctggatgctc tccagcgcat gggcgatgag attgcgctcg
     8761 aagaccgcat agatggaacg ccccaggccg atgatctcgc cgccaccggc cgccgcgggc
     8821 gcatctgaca aggccttgaa ctgcagccgg gcgcggttgt tgtagagcag gacacggcca
     8881 tcgaggttgc acaccacgac gctctgggtc agttccgaca tcagtgcggc gaggcggttg
     8941 cgctcctcct ccacggacag gcgggcttcg cgaatgcgcg cttccacgtc gtcctgaaga
     9001 ctgcggcgct ggtccgcgag cgcattcacg gcatccgcaa tgctgttcag gtcgggatcg
     9061 gcctgcgcct tgaggcgccg cgccggatct gtcccgacga tcaggccgat ctcgtcggcg
     9121 agcaatgcag gcgcagccac atgggcctgg acgaaggact tcatcgccac ggcgacggca
     9181 ccgcagcccg ccagaccgat catgatcagc agaccgagcc ggggattgag gatgggctcc
     9241 agaacggcct gttcatcgac gcccagatcg cgccacaggg ccacggccgc agaaatgagg
     9301 atggcagcca ccatcccgca ggcggccaga atcggccacg cctgccgggc cttggggctc
     9361 acgcgggcaa ttccagcagg gatttgaccc gcgccaccag ttccttggta gagaacggct
     9421 tggtcatata ggcgtcggcc cccatggcca gccccttgct cacttcggtc tcacggccct
     9481 tggcagtgag catcagaatg cgcacgcctt gcagcgcgcc gtcggcacgg atctcctgac
     9541 agacttcgaa accgttcttg cgcggcatca tcacgtccag cagcaccaga tcgggcgatt
     9601 cgctgcgtac cttgtcgagg gcctcctgac cgtcgttggc gatacagacg gcaaaaccct
     9661 cgcgtttcat caggaattcc agagagatga cgatgttttg ctcgtcatcg gcgatcagta
     9721 tttttttggt cattcagtcc cccctcctta tgtctccgca ggtccgatcc gcgcacacgc
     9781 gaacctctgc cccggttggc tcgattgtat agtttatccc gctctctctt gcgcttctgt
     9841 tgatctgccg caaggcaaga aaaaggagaa ccgcgcgccc tcacccggag cactctccac
     9901 ccacagacgc ccgccataat gctccacgat ctgccggctg atcggcaggc cgagaccggt
     9961 gccctgcggc ttggcggtca tcgtgtttcc cccctgacgg aatttttcaa agacgaccgt
    10021 ctgttcgtcc aacgtcagcc cggggccatt gtcgctgacg tccacctgca cgccgtccga
    10081 cgtttccgcc agcttcacgc ggacctgacc gccgtcccgc tccacgaact tcacggcgtt
    10141 ggacaagagg ttgagcatca cctgaatgag ccgatcccgg tccgccagca ggaccggcag
    10201 cgacgcgggc agatcgagct cgaggctcac accccggtcc ttgaacaact ggcttgtgga
    10261 cgccacggcc tgttccacca cctccaccag attcacctcg ccgttgtgcc attccgcgtg
    10321 accggactcg atcttggcca tgtcgagcac ctgattgacg aggcgggtca gccgttcggt
    10381 ctcggccacg atgatgccca gaaagcgttt gcgatcggcc agatcgatgc gcgggtcttc
    10441 cagcagcatc tcggaaaagg cacggatcga ggtgaggggc gtgcgcagct catgggtcac
    10501 ggacgacatg aaatcgtcct tgaggcggtc gagttccttg aggcggtcgt tggcatggcg
    10561 caactctgcc gtggcggctt ccagctcccg ggatttctgc tcaaggcgct gggaataggc
    10621 acggacctgg gaggcctcgt ccagaatgtg catcacctcg tccagcccga gcggctcttc
    10681 cttgacggcc gacgccacca tcacgcgagc cgacgcaccg ccgatggcac ccgccagggt
    10741 cgtttccgca aagtgaacca ggccggcgtc cgcctccagg tctgcaacgc gctcgacgcc
    10801 gcgctcgcgg gcgtaggagg agaacagggc ctgggcgcgc tccagtccca cgaaacgggc
    10861 aatcagggga agcaggtcgc cgacccgcgc gctgccgcgc cagaaagccc ccccgctggg
    10921 ccgtccctgg cggaagacat ccacgaacaa ggtcgcctgc ccggcttcca ccgcgttggg
    10981 cggcctcaac agggacaccc acgtatacgc gcccacattc gcaaacaggc tccagaacaa
    11041 gctgtgggcc atgggatcca gtccgcgcag accaaacagt tcctgcggtc gcaggaactc
    11101 gagtccgaac agccccccct gcaggaaatc gccgggcagc cagcctgatt tggcgaatga
    11161 cggcagcagg agcgtgtagc cccatacgag gaagccggca ctgagtcccg ccagcgcccc
    11221 cgcccgggac cctccgcgcc agtacatccc acccagcatg gccggcgcaa actgggcgac
    11281 cgcggcaaag ctgatgagac cgatcgacac cagcgcatgc gcctcgcccg ccaacctgaa
    11341 atacaggtag gccagcagca ggatcaacac gatggcgccc cgccggatcg ccagcaggag
    11401 gccagaaaga tcgttgtgcc cgcgcagcgc cccccggctg aatcggagca gcgccggcat
    11461 caccagatca ttgcagacca tcgtggacag ggcgatcgtt tccacgatca ccatcccggt
    11521 cgccgccgac aatcccccga tgaagaccag cagcgccagc aggggatgac ccgatgccag
    11581 cggcagggtc aggacgaagg tgtccgcatc caccgcctgc ccacggaagt aaatcagtcc
    11641 gccgagggcg atgggcaaca cgaacacgtt gatcagcatc atgtacaggg ggaacagcca
    11701 gatcgcccga cgcaggtgct tctcgttgac gttctcgacc accgcgacct ggaactgacg
    11761 cggcaggaac atcaccgcca gcatcgacag cacggtcagc gcaaaccagt ttccgtaact
    11821 gttgccgccc gtttcgacga ccatcaatgc ccggagggcg ggatcctcgg cagcctggga
    11881 aaacagatcg ccgaaaccgt cgaacagtcc gaacgtgacg aagccaccca ctaccaggaa
    11941 gatcaccagc ttcaccagcg actcgaacgc gaccgctgcc accagcccct cgtggcgctc
    12001 cgtggcatcg agatgacggg tgccgaacag gatcgtgaat gcggccatca tcagcgcgac
    12061 atagaacgtg gtgtccgtca tccatggcag ggcgccggtc cggatcggcg tcagaaggcc
    12121 ctgaggatgg atgaggatcg aaaaactgcc ggagaccgcc ttcagctgca gcgcgatata
    12181 cggaatcacg cccagcaccg cgatcaccgt gaccagcccg ccgagcagat ggctcttgcc
    12241 gtaacgcgat gcgatgaagt ccgcgatgga ggtgatccgg ttcgacttgc cgatgcggat
    12301 catcttcagc atcacgaacc accacaatcc caccaccagc gtcggcccga gaaaaaccgg
    12361 cacgaaacct atccccgacg atgccgcccg gccaaccgaa ccgtaatacg tccaggttgt
    12421 gcaatacacc gccagggaca gcgcgtagat cgtggggttg gcgatcaccg agcgcccggc
    12481 atctgcccgc ttgtcaccgt aataggcaac tccgaacagg gccgccagat aaaggaagga
    12541 caccgcgaca acggttgtgc cggacagcat cgcctatcct ctttcaggta cgggaatgcg
    12601 gcaggtgtgc atcatcgctc gacgatccac gccatcagca cgattaccgc cgcccacagc
    12661 ccgaacacat aaagatagag cagcgggatt ccctgccagg cgccatcgtc attgaagatc
    12721 gacagcaccg gaaaattggc cagcacacat cccagcacga ataccgcgac cagccgctgc
    12781 cccatcaggg tcgccttgat catccctgct cctccgtttt ctttttatgt ccggcatcga
    12841 gcgcgtccgc cggtgccaga tttataaacc gaatcggcgg gcttggcgag aaccttcggc
    12901 atcgcccggg cggaggaccc acttcccggc gtagacgaga aaaaggcgcg aaattcgcgc
    12961 ccttttctcc ccctcttgcc cggctcatta ggccgggttc ctccccatcg ttcctctcac
    13021 gtcccgcact ggatttttat gccggcgact cggggtgaat cgccggtagc aggacgtgcc
    13081 cggcctgacc ggccggcaca caatgcttac ttgagctgat ccagaatatg cgggttttcc
    13141 agcgtcgagg tgtcctgggt gatctcctcg cccttggcca gctgacggag caggcggcgc
    13201 atgatcttgc cggaacgggt cttcggcagg ttttcaccga agcggatgtc cttgggcttg
    13261 gcgatcgggc cgatttcctg acccacccag ttctgcagct ccttgaccac cttggcggct
    13321 tcgtcgccgg acgggcgggc acccttgagc accacgaagg ccacgatggc ttcgccggtc
    13381 aggtcgtccg gacgaccaac cacggcggct tcggccacct tctcgtgggc caccagggcg
    13441 gattcgattt ccatcgtgcc catacggtga ccggagacgt tcagcacgtc atcgatacgg
    13501 ccggtgatgg tgaagtagcc ggtgtccttg tcgcggatcg caccgtcgcc cgccaggtac
    13561 agcttgccct ggaagtcggc cgggtagtag gacttcacga accgctccgg atcgccccag
    13621 atggtgcgga tcatggacgg ccacggacgc ttcacgacca ggatgccacc ctggccccag
    13681 ggcacgtcgg tgccggtctc atcgaccacc gccgcctgga tgcccgggaa gggcagcgtg
    13741 caggagccgg gaaccagcgg cgtggcgccg ggcatcggag tgatcatgtg gccgccggtc
    13801 tcggtctgcc agaaggtatc gacgatcggg cagcggctgc cgccaacgtt ctcgtaatac
    13861 cactcccacg cggcggggtt gatgggctcg cccacggaac ccaggatgcg cagcgaggtg
    13921 agatcgtagt tcttcgggtg caccgccggg gtggtgtcgg aggccttgat cagggaacgg
    13981 atcgcggtcg gggcggtgta gaacacggag accttgtggt cctggatcat cttccagaaa
    14041 cggccggcat ccgggtaagt cggcacacct tcaaagacga tctcggtggc accgcaggcc
    14101 agggggccgt aagtgatgta ggtatgaccg gtgacccagc cgatgtcggc ggtacaccag
    14161 aagatgtcgt tcggcttgat gtcgaacgtg tacttcatgg tcaggatggc gtgcagcagg
    14221 tagccgccgg tggagtgctg gacacccttc ggcttgccgg tggagccgga ggtgtagagc
    14281 aggaacaggg gatgctcggc ctcgacccat tccggttcgc agacatcgga ctgctgggcg
    14341 acgatgtcgt cggcccacac gtcacgacct tcgaccatgg cagtttcgcc gcccgtgcgc
    14401 ttgaccacca gcaccttctg gatggacggg cagccgctgg cgagggcttc gtcggcgatg
    14461 gccttgaggg gcagcgcctt gccaccgcgg ttctggccgt cggaggtgat cagcaccacg
    14521 gcgccggcgt cattgacgcg gtcggccagg gactgggcgg agaagccgcc gaacacgatg
    14581 gagtgcgtcg caccgatacg ggcacaggcc tgcatggcga ccacgccttc gatggacatg
    14641 ggcagataga tgacgacccg gtcacccttc ttgacgccca ggctcttcag cgcattggcg
    14701 aacttgctga cgcgggtcag cagatcgcca tacgtcacct tggtgacatc gcccttgtcg
    14761 ccttcgaaga tcatggcgac cttgtcgccc aggccattct tgacctggcg atccaggcag
    14821 ttgtaggaga cgttcagctt gccatcggca aaccacttga agaacggcgt attgctctca
    14881 tccagcacct gggtgaaagg ggtctgccat tcgatcagtt ccttggcgcg cttgccccag
    14941 tagccttcgt aatcggcttc cgcttcggcg cacagcgcct tgtacgcttc catgcccgac
    15001 acggcagcgt ttttgaccac ctcttcagac ggatagtaaa gtttctgggt ggtttcgctc
    15061 gtcatttttc acctctcctc gtgttgtgag tgtgcaacgt agtggatttg aaaccttgtt
    15121 gggcggatgc aggaccagac gcgccgtcca ctgaccggat tgagttctgt ttttcttgta
    15181 ctgcgtattt ccgatcgagt ccgcccgaga ttaaaggcgc ttaatcttac acaggactta
    15241 caccctctcc acgagatttt ttcatttccg cagaccgcca cccgacacgc cgtcaccgcg
    15301 ccgcccggag gccacgtcgc gccgcaatta ttgcggcaac gcagcaattt tcagtcatcg
    15361 accgggaatt cagaaagcat cgacaaagcc cggaaactgt catattttat acgcatacac
    15421 cccggattcc tgttgatgcg tatacgtctc tctgcctgcg caatcctgct cctgagcacc
    15481 agcgcctgtg cgccaatgct gtccacccga gcccccgcgc catccgaaag ccatatccag
    15541 gacaggaatg cggcatttcc cgcaggaacg attccgcccc ttgccgggcc gagcctgatc
    15601 ctgccccgcc cgcaagccac gccgcgcccc gagacattct ccgtcgtggt gcacaatgtg
    15661 cggctccagg atctcctgtt cgcgctcgcc cgcgacgccc gcctgaacgt ggacatccac
    15721 ccgggcctga acggcacggt caccctcaac gccatcgacc aaaccctgcc ccagatcctg
    15781 acgcgcctcg cccgacaggc cgacctgcgc ttcgaactcg acggcaccca cctggcggtg
    15841 atgcccgaca gcccctacct gcgcacgtac cgcgtcgatt acgtcaatct cagccgcagc
    15901 gtcaccggca cggtctccac caacacccag atcgccacca gcggcagcgg ctcggccgtc
    15961 agcggcagca ccacactcca gacggccgcc ctccccaacg gcggcaacgt ttcggccacc
    16021 cgcatcgaaa acgcctcccg caaccagttc tgggaatcca tcgagcgcaa catccgcgac
    16081 ctgctgcggg aaaccgacaa gctcctcccc gaaggctcca gcgacaccgt catcgaaaac
    16141 ctcgagacca ccagcaccag cggcaccggc gtacagccct ccgccacggc cgcccggagc
    16201 cggacgcgca ccacctccgc gtcaccgcaa gccggcatcg cggcgagtcc caatcccgcc
    16261 cttttacaga acagcgccac cacggtggtc cacaaggcca ccttccgcga agccgcttcc
    16321 gtcatcgtca accgggaaac cggcgtcctc actgtgcgcg ccaccggccg ccagcacgaa
    16381 aaggtgcagg aattcctcga tcaggtggtg gcggccgccc gccgtcaggt gctcatcgaa
    16441 gccaccatcc tcgaagtcgg cctgtccgac acctatcgtc agggcatcga ctggaaccgc
    16501 gtgctctcga gcggcacccg tttcggcatc accggaccga ccctcggcag cagcgccggc
    16561 aatgccgtca atcccttcag cgtcgcctac aagagcgccg acggcatcga ctccacgatc
    16621 cgcctgctcg aaggcttcgg caccgtgaag gtcctctcca gccccaaact gtcggtcctc
    16681 aacaaccaga cggcggtgat caaggtggtc gaggaatacg tctatttcaa cgtcaaggcc
    16741 gacaccaccc agaccaccaa cgcgggcgcc ctcaccaccg tcaccaccac gccccaggcc
    16801 gtctcagtgg gcctcgtcat gaccgtcacg ccccaggtca gcgaggctga cgagatcatc
    16861 ctcaacgtgc gccccaccat ttccagcatc gccgacttcc gccgcgaccc caaccccaac
    16921 attcccgcca cgattcccaa cctcgtcccc cagatccgca cccgcgagat cgaatccgtg
    16981 ctgcgtatcg aaaacggcga aaccgccgtc ctcggcgggc tcatggaaga ccgggtcgac
    17041 ttcaagaccg gccgcgtccc cggcgtcggc gccgtgccgc tgctgggtga ggtcttcacc
    17101 acccgcgaca acctggtcca gaagaccgag ctggtcatct tcctgcgccc ggtggtggtc
    17161 cgcgacggca atctcctggc cgccagcccc ctgcgcaaca gcctcccccg cgacgacttc
    17221 ttccgccgcg gtccgctacc cggcacccgc aactttcctg acaccacccc atgagcctgc
    17281 tcctggaagt cctgcgcaag cacgaggccg aacggcgcgg cgaaacgaca ccgcccggcg
    17341 cctccgcgcc gccacccccc cccccccccc cccc
//
LOCUS       DAOQPA010000079        17011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1695_length_17011_cov_18.760793, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000079 DAOQPA010000000
VERSION     DAOQPA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1695_length_17011_cov_18.760793"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="PKX01_11450"
     CDS             <1..1887
                     /locus_tag="PKX01_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMZ76787.1"
                     /translation="LGGRVRLFAAHSLEQEARAVAATVRGWLASGKRRIALVAADRVA
                     ARRTRALLERDGVLVEDETGWKLSTTRAAALIDAWLSVLSSDGYHRDVLDLLKSPFVA
                     GSTGEADRAAGIEAVARLYAHHGLASGLDAVALLAAGDALAAALVRLLLEARSVFPRT
                     AATPAQWLDRLGAALAALGAVTALASDAAGADILAWIAARRAELADEDARIGFHEWRQ
                     WLDRELESVMFRDRSVDSPVVMTHLAAARLRRFEAAALIGADADNLAPQDRRPVFAHE
                     GVRAELGLPGSAQARARLHDDLAGLLTLADAVCISWQHLRDGEAVLPCAAVDVLSLVH
                     QQAFGESLRAPVPGSFEADVPVVGQPVPAPVLMPEQVPTRISASGLASLIACPYRYFA
                     RHVLGLGEAEEVSEALEKADYGQMIHAILQAFHARFPQIATEDDAVLVAALEDESARV
                     FGPALARNFLEHAWAARWRAQLPSYVAWQKAREAEGWTFAAAEESAWRDFALADGARV
                     TLHGRLDRIDRRRDGTEAVLDYKSRPLGALRKQAADVDDVQLAVYTLLRGTAVREAAY
                     VALDDGAVGAAPLDDPVAMAEVQAERLVTAFDALHAGAPLPAHGVACTHCEMRGLCRK
                     DYQA"
     gene            1884..5189
                     /locus_tag="PKX01_11455"
     CDS             1884..5189
                     /locus_tag="PKX01_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ76788.1"
                     /translation="MSVPHDSPVERARRALDPARSVVVEACAGSGKTWLLVSRLLRLL
                     LAGARPGEILAITYTRKAAREIEERLRQWLRLLAGAPDAAVLDFLSERGLEAAAAQAA
                     LPRARGLLEAVLGAEPPMTITTFHGWFARIVGGAPFGSGLAGFRLSEAEAPLLDEAWA
                     RFARSCAAEPAGVEAAALLRLFDTVGAARTRALLQAFVNRRAEWHAYLDDGRDGLDVA
                     LARLQADLGVVMPGAALPAFLATAGLAEAVEALARLLDANTDSDRARAAALRAGFAAD
                     DAATRFAALKAAVLTQKGEPRAVKASAAQAKRLGEAGQTRFLDLHGRLTDQILAAADA
                     VLEEHIFVLNRDVLVAAGGLLHALEAHKAERRLMDFADLEWHTDRLLSDPEQAPFLQA
                     RLDARYRHILLDEFQDTNPLQWRILVAWLDAYGLDGTPPRIFMVGDPKQSIYRFRRAD
                     FRIFTHAAEWLARRFDADRLANDATFRNAPAVVEVVNSLFAHEPAFVGFRPQTAVRAG
                     LPGQVRLLPLIEDDEAAGGTASNVATDALRDPLRVAPVLAENLRRRREALAVAEHISA
                     LVGRWQVAGEHGPRPARYGDVLILTRRRSVLPEFERALRDAGIPYLSVSRGGLLKTLE
                     AADLVALLRWLASPADDLALVHALRSPVFGVSDDALLRLAARPEAGWWARLGAMLAEG
                     AADAALERAGHRLADWKTQAATLPVHDLLDRIFHEGQVLARYHAVVPPPMAAGVRANL
                     EAFIALALELDGGRYPSLPRFIDELGRLSGAGDDEAPDEGLIPGNGDDADGRVRIMTI
                     HGAKGLEAPVVWLIDARNGHQKPDTDQVLLDWPPGDIRPRHFSLATRKDERGRCRAAL
                     FEAEEQARAREDLNLLYVAITRASQYFLASGSLPTRDAAAPNPYQRIAAALGRLGAEA
                     GTWGSPPVEVAAAPAPAPAVAAEVAPTIAPVPVGQYRSVADAVAESAGTLFGTAFHTA
                     LECLTEARSTAGLDPAAVELAGRLVARPQLARFFDPAACRWARNEVEFVDAAGRLGRI
                     DRLVDDGETLWVVDYKTGAPDERLLDVYRAQVRTYGRAVAALWPGRPLRAVLVFAGGE
                     ALEVAVDEAGNAA"
     gene            5285..5734
                     /locus_tag="PKX01_11460"
     CDS             5285..5734
                     /locus_tag="PKX01_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ76789.1"
                     /translation="MLVSEILAIKGKVLFTIAANKTLADAVAVMTEQDVGSLVVFDKG
                     HMAGLLTFREVLVATQKAGAGWESLPVTEAMLKTPVVANPSMEMDELRRQMVEHHQRY
                     LPVMDDTMLMGVVSFHDVAKAVLEEQSFENRMLKNYIRNWPSEEGQV"
     gene            5969..7642
                     /locus_tag="PKX01_11465"
     CDS             5969..7642
                     /locus_tag="PKX01_11465"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HMZ76790.1"
                     /translation="MDKIWLQSYPPGVPPEVDLDEFSSLVDLFEQSVRKFAARTAYVN
                     MGKSLTYGELDELSARFAGWLQGELGLGPGARVALMMPNCLQYPVAMFGVLRAGLTVV
                     NVNPLYTARELEHQLKDAGCEAIVIVENFAHTLQDVLPSTPVRHVVTTGLGDLLGFPK
                     SLLVNFVVRHVKKMVPAWSLPGSIAFREALAKGATHPLRPVMLGHEDLAFLQYTGGTT
                     GVAKGAMLTHGNIVANLQQAHAWIGPYVREGEELIVTALPLYHIFSLTANCLTFFKIG
                     AENLLITNPRDIPGFIAELAKVRFTAITGVNTLFNALLNHPDFEKLDFSSLRLALGGG
                     MAVQQAVAERWKAVTGRPLAEAYGLTETSPAVCINPLDLPEFNHSIGLPISSTDVSLR
                     DDEGYEVLLGEPGELCVKGPQVMKGYYNRPEDTARVFTRDGYLRTGDVAVIAPDGYVR
                     IVDRKKDMILVSGFNVYPNEVEDVVASHPGVLEVAALGVPDERSGEAVKIFVVRKDPT
                     LTAEALIAHCRQGLTGYKVPHYVEFREELPKSNVGKILRRALRDASTPK"
     gene            7959..9623
                     /gene="ilvB"
                     /locus_tag="PKX01_11470"
     CDS             7959..9623
                     /gene="ilvB"
                     /locus_tag="PKX01_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HMZ76791.1"
                     /translation="MMQMTGADLIMKLLERQGIRTVAGVPGGAALPMYDALTRSESIH
                     HVLCRHEQGAGFIAQGMARVSGRPEVCLASSGPGATNLITAIADAKLDSIPIIALTGQ
                     VPLSMIGTDAFQEVDTYGLTIPITKHNFLVRSAKELLTVIPAAFRIAMSDRPGPVLVD
                     VPKDVQNQMISFEDFPDVVVPDAPPPVDIAAVEEAARMIEAAERPVLYLGGGVIHSGA
                     SQLAVTLAEQASLPTTMTLMALGAMPMDHPLAIGMLGMHGARYTNYVLEESDLIICVG
                     ARFDDRAIGKAAEFCPNAKIIHIDIDPSELHKIKTAHVAIAGDVGQVLGELLPRVKPQ
                     LRKRWLSHVESLKGRFPLAMPGVDDPRTPYGLIHAVASCLDDNAIITSDVGQHQMWVA
                     QAYPFRRARQWLNSGGLGTMGFGFPAAIGAALAEPERTVVCFSGDGSLKMNIQEMATA
                     AQENVNVKIVLFNNQSLGLVHQQQNLFYGKRIMASRYEHPTDFLKIAEGFGMRAVNLD
                     PSPNPRATLAEALNTPGPCLIHATIDPNEFVYPMVPPGAANSEMIG"
     gene            9642..9977
                     /gene="ilvN"
                     /locus_tag="PKX01_11475"
     CDS             9642..9977
                     /gene="ilvN"
                     /locus_tag="PKX01_11475"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMZ76792.1"
                     /translation="MSDQTSQLSEYIPDVPEHTGFHKVVLELAVANHPGVMSHICNLF
                     ARRAFNVEGILCMPVGEGGTSRIWLLIFEDERLEKMIRQVEKLEDVLYVRQHAGDHPV
                     FGRLDEFFQ"
     gene            10082..11224
                     /gene="aroC"
                     /locus_tag="PKX01_11480"
     CDS             10082..11224
                     /gene="aroC"
                     /locus_tag="PKX01_11480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ76793.1"
                     /translation="MSGNTLGTLFTVTSFGESHGPAIGCVVDGCPPGLALCEADIQAE
                     LDRRKPGTSRHVTQRREPDTVEILSGVFEGQTTGTPIALLIRNQDQRSKDYGNIAETF
                     RPGHADYAYWEKYGIRDHRGGGRSSARETAVRVAAGAVARKWLAERYGIVIRGYMAQL
                     GPVEIPFAAWDDVDDNAFFAPNAALVPALEDYMDELRKSGDSVGARINVVATGVPVGW
                     GEPVYDRLDADIAHAMMGINAVKGVEIGAGFASVAQLGTQHSDEMTPEGFLSNQAGGV
                     LGGISTGQDILVSIAIKPTSSTRLDRRSINKAGEPVVVNTHGRHDPCVGIRATPIAES
                     MLAIVLMDHALRHRAQNADVACPTPPVAGLAPDGVQGVPSPKGSKG"
     gene            11314..11556
                     /locus_tag="PKX01_11485"
     CDS             11314..11556
                     /locus_tag="PKX01_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HMZ76794.1"
                     /translation="MEQHDLNHEFPEYRDQIHSLKVSNNHFSRLFDEYHDVNRHVVRV
                     ELNAELATDFELEELKKRRLKLKDELYDMLKGAVVN"
     gene            11663..12808
                     /locus_tag="PKX01_11490"
     CDS             11663..12808
                     /locus_tag="PKX01_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ76795.1"
                     /translation="MIPYWRLSGYYFFYFAFVGAFSPYFSLYLQSIGRSAAQIALLMS
                     LLSVMRLVAPNLWGWLADRLGARVPIVRLSAVLSLAGFAGFLMTESPVGLFASMAVLA
                     FFWSAALPLVEALTFSHLGAHASRYGSIRVWGSVGFILAVLGLGYVLDGAPIRTLLWI
                     TLGLLGGILVYAAVLPEGAAPAHSMNPASLGEVLRRREVRALLTACFFMSAAHGALYV
                     FYSVHLVQLGHHKTLVGWMWTLGVVAEIAVFMAMPQILRRYSLRGILLFSFAVAVVRF
                     LLIGWGANSLAVLILAQIGHGATFGAYHAAGVAAINRWFGPQLQARGQALYGSVSFGA
                     GGMVGGVVSGYTWDSIGAGWTYTLGSAFALMGLAVLLAGWRPAVREA"
     gene            12833..13681
                     /locus_tag="PKX01_11495"
     CDS             12833..13681
                     /locus_tag="PKX01_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ76796.1"
                     /translation="MMSVWKGMLPLGAAVLMVTACQTVQTTQSGAVGVERRQSMLVSS
                     REMEQASAQSYHKILQQAAEKNALNRDRVQVERVRVVARRLIPATGAFRPDAPGWRWE
                     VNVLTSEELNAWCMAGGKIAFYSGIIERLQLSDDEMAAIMGHEIAHALREHARERASQ
                     AMVTNIGVSIVGAVLGAGQAGTNLIGQVANVSFGLPNSREQETEADRIGVELAARAGY
                     DPRAAIVLWQKMAKVSNGAPPQWLSTHPSNETRQADLQQYAARVMPFYEEARNARPTQ
                     GSKPVK"
     gene            13711..15120
                     /gene="leuC"
                     /locus_tag="PKX01_11500"
     CDS             13711..15120
                     /gene="leuC"
                     /locus_tag="PKX01_11500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMZ76797.1"
                     /translation="MHAQTLYEKLWSSHVVHVEDDGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRVNSIVATADHNTPTDHWDQGIQDPVSRQQVETLDANIREVGALAYFPF
                     MHERQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVMATQCL
                     LQKKSKTLLVRVDGALGPGVTAKDVVLAIIGRIGTAGGTGYAMEFGGSAIRSLSMEGR
                     MTVCNMAIEAGARAGLVAVDDKTIAYLKDRPFSPRGDQWDRAVTYWRTLHSDEGAQFD
                     AVVVLNGAEIKPQVTWGTSPEMVVPVDGSVPDPALEKDPVKREGMERALQYMGLTPRT
                     PINKVAVDKVFIGSCTNSRIEDLRAAAAVARGRHKADSVRQVLVVPGSGLVKHQAEAE
                     GLDKIFIAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAAIAGHFVDVRNLA"
     gene            15140..15265
                     /locus_tag="PKX01_11505"
     CDS             15140..15265
                     /locus_tag="PKX01_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HMZ76798.1"
                     /translation="MRKFVALMGVFAVLLLTGCNTVHGIGKDIEKGGEAIQKVAK"
     gene            15265..15903
                     /gene="leuD"
                     /locus_tag="PKX01_11510"
     CDS             15265..15903
                     /gene="leuD"
                     /locus_tag="PKX01_11510"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HMZ76799.1"
                     /translation="MKPFTRLDGLVAPMDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDIGQPGMDNTGRPKNPEFVLNQPRFQGAQILLTRDNFGCGSSREHAPWALEDF
                     GFRVLIGTSFADIFFNNCFKNGLLPIRLPAAEVDALFGQCLATPGYRLTVDLAQQTVT
                     RPDGKAIPFEVDPFRRECLLNGWDDIGLTLRHADKIREFEARRRIEQPWLFA"
     gene            15950..17011
                     /gene="leuB"
                     /locus_tag="PKX01_11515"
     CDS             15950..17011
                     /gene="leuB"
                     /locus_tag="PKX01_11515"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HMZ76800.1"
                     /translation="MKILVLPGDGIGPEIMEQALRVLDALRGDGLSFEVEHGLLGGSA
                     VDATGTPFPEATRALARAADAVLLGAVGGPQWDALPRDQRPERGLLGIRKELNLFANL
                     RPAILYPELANASTLKPEVVAGLDILIVRELTGDIYFGQPRGVREENGERVGFNTMVY
                     SESEIRRIGKVAFEAARKRDKRLCSVDKMNVLECTQLWRDVMIELSADYPDVALSHML
                     VDNAAMQLVRAPKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPS
                     HGSAPDIAGKGVANPLATILSAAMMLRYTFNLEAPAARIESAVKKVLAAGYRTGDIYE
                     AGTRRVGTQEMGDAVIAAL"
BASE COUNT         2626 a         5810 c         5755 g         2820 t
ORIGIN      
        1 ctgggcggcc gcgtgcgtct cttcgcggcc cacagcctgg agcaggaagc ccgtgccgtg
       61 gccgcgacgg tgcgcggctg gctggcgtcg ggcaagcggc gcatcgccct ggtggcggct
      121 gaccgggtcg ccgcgcggcg cacccgcgcc ctgctggagc gggacggcgt cctggtggag
      181 gacgagactg gctggaagct gtccaccacc cgcgccgcgg ccctgatcga cgcgtggctg
      241 tcggtgctgt cgtccgatgg ctatcaccgg gacgtgctgg atctgctcaa gtcgcccttc
      301 gtggcgggca gcaccggcga ggccgacagg gcggcgggca tcgaagcggt ggcgcgcctg
      361 tacgcccatc atggccttgc ctccggcctg gatgccgtgg ccctgctggc ggcgggcgat
      421 gccctggccg cggccctggt gcgcctgctg cttgaggcgc gttcggtgtt tccccgcacc
      481 gcggcgacgc cggcccagtg gctcgatcgg ctgggtgcgg cgctggcggc cctgggtgcg
      541 gtgaccgccc tcgcatccga tgcggcaggg gccgacatcc tggcgtggat cgccgcccgc
      601 cgtgccgaac tggcggacga ggacgcgcgc atcggttttc acgagtggcg ccagtggctt
      661 gaccgggagc tggagagcgt gatgttccgc gaccgcagcg tggacagccc ggtggtgatg
      721 acccatctgg cggcggcccg cctgcgccgc ttcgaggcgg ccgccctcat cggcgccgac
      781 gccgacaacc tggctccgca ggatcgccgc ccggtgttcg cccacgaagg cgtgcgcgcg
      841 gagctgggcc tgcccggatc ggcccaggcg cgggcgcgac tccatgacga cctggccgga
      901 ctcctcacgc tggccgacgc ggtgtgcatc agctggcagc acctgcgcga cggcgaagcc
      961 gtgctgccct gtgcggcggt cgatgtgctg tccctggtgc accagcaggc tttcggcgag
     1021 tccctgcgtg cgcccgtgcc agggtcgttc gaagccgacg tgcccgtcgt cggacagcct
     1081 gttccggcgc ccgtgctcat gccggagcag gttcccacgc gcatctccgc cagcggtctg
     1141 gccagcctga tcgcctgccc ctaccgctac ttcgcccgcc acgtgctggg cctgggcgag
     1201 gcggaggagg tgagcgaggc cctcgagaag gccgattacg gccagatgat ccacgccatc
     1261 ctgcaggctt tccacgcccg cttcccgcag atcgcgacgg aggatgacgc ggtcctcgtc
     1321 gccgcgctgg aggacgagag cgcgcgcgtg ttcggcccgg cgctcgcccg caacttcctc
     1381 gaacacgcct gggcggcccg ctggcgggcg caactgccgt cctacgtggc ctggcagaag
     1441 gcgcgggagg cggagggctg gaccttcgct gccgccgagg aaagcgcctg gcgcgacttt
     1501 gccctggccg acggcgcccg cgtgaccctc cacggccgcc tggaccgcat tgaccggcgc
     1561 cgggacggca ccgaggcggt gctggattac aagtcgcggc ccctcggtgc gctgcgcaag
     1621 caggccgccg acgtggacga cgtgcaactg gcggtctata ccctgctgcg tggcacggcc
     1681 gtccgcgagg ccgcctacgt ggccctcgac gacggggccg tcggcgccgc gccgctggac
     1741 gatcccgtgg cgatggccga agtccaggcg gaacgcctcg tcaccgcctt cgatgccctg
     1801 cacgccggtg caccgctgcc ggcccacggc gtcgcctgca cacattgtga gatgcgcggc
     1861 ctgtgccgca aggattacca ggcatgagcg tgccccacga ctcgcccgtc gagcgtgccc
     1921 gccgcgccct cgatcctgcc cgcagcgtgg tggtcgaggc ctgcgccggc agcggcaaga
     1981 cctggctgct ggtgtcgcgc ctgctgcgcc tgctgctggc cggcgcgcgg cccggcgaga
     2041 tcctggccat cacctacacc cgcaaagccg cccgcgagat cgaggagcgc ctgcgtcagt
     2101 ggctgcgcct gctcgccgga gcgcccgatg cggcggtgct ggacttcctg tccgagcgcg
     2161 gactggaggc cgcggcggcc caggcggcgt tgcctcgcgc ccgcggcttg ctggaagccg
     2221 tgctcggggc cgagccgccc atgaccatca ccaccttcca tggctggttc gcccgcatcg
     2281 tcggcggtgc gcccttcggc agcgggctgg cgggcttccg cctcagcgag gctgaggcgc
     2341 cgctgctgga cgaggcctgg gcacgcttcg cccgcagctg cgcggccgag ccggcgggcg
     2401 tcgaggccgc cgccctgctg cgcctgttcg atacggtggg cgccgcacgc acgcgggcgc
     2461 tgctccaggc cttcgtgaat cgtcgcgccg agtggcacgc ctacctggac gacggtcgcg
     2521 acgggctcga tgtggccctg gcgcgcctgc aggccgatct gggcgtggtc atgccggggg
     2581 ccgcgctgcc agccttcctt gcgacggcgg ggctggcgga ggcggtggag gcgctggcac
     2641 gtctgctcga cgccaacacc gacagcgacc gggcccgcgc cgctgccctg cgggccggct
     2701 ttgccgcgga cgatgctgcc acgcgcttcg ccgccctcaa ggcagcggtc ctcacccaga
     2761 agggcgagcc ccgcgccgtc aaggcctccg cggcccaggc aaaacgcctg ggcgaggccg
     2821 ggcagacgcg cttcctcgac ctccacggac gcctcacgga ccagatcctg gccgccgccg
     2881 atgcggtgct ggaggagcac atcttcgtcc tcaaccggga cgtgctggtc gctgcgggcg
     2941 gcctgctgca cgccctggag gcccacaagg cggagcgccg cctgatggat tttgccgacc
     3001 tggaatggca caccgaccgg cttctctccg atcccgagca ggcgcccttc ctgcaggcgc
     3061 ggctggatgc ccgctaccgc cacatcctgc tggacgagtt ccaggacacc aacccgctgc
     3121 agtggcgcat cctggtggcc tggctggacg cctacgggct cgacggcacg ccgccgcgga
     3181 tcttcatggt gggtgacccc aagcagtcca tctaccgctt ccggcgtgcc gatttccgca
     3241 tcttcaccca cgccgccgaa tggctcgccc ggcgcttcga cgcggaccgc ctcgccaacg
     3301 acgccacctt ccgcaatgcg ccggccgtgg tggaggtggt caattccctg ttcgcccatg
     3361 agccggcttt cgtcggcttc cgtccccaga cggcggtgcg tgccggtctg cccgggcagg
     3421 tgcgtctgct gccgctgatc gaggacgatg aggcggcggg tgggaccgcg tcaaacgttg
     3481 cgacggacgc gctgcgcgat cccctgcggg tggcgccggt cctcgccgaa aacctgcgcc
     3541 gtcgccgtga ggcgctggcg gtggccgaac acatctccgc actggtcgga cgctggcagg
     3601 tggcggggga gcacggtccg cgtccggccc gctacggcga cgtgctgatc ctgacccgcc
     3661 ggcgcagcgt gctgcccgaa ttcgagcggg ccctgcggga cgccggcatt ccgtatctca
     3721 gcgtgagccg tggcggcctg ctcaagaccc tggaagccgc cgatctggtg gccctgttgc
     3781 gctggctggc gagccctgcc gacgatctgg ccctggtgca tgccctgcgc tcgccggtgt
     3841 tcggcgtgtc tgatgacgcg ctgctgcgtc tggcggcccg tcccgaagcc ggctggtggg
     3901 cgcgccttgg cgccatgctg gcggaagggg cggccgacgc ggcactggag cgcgccgggc
     3961 atcggctggc cgactggaag acgcaggccg cgacgctgcc ggtgcatgat ctgctcgacc
     4021 ggatctttca cgaaggccag gtgctggccc gctatcacgc cgtggtgccg ccgcccatgg
     4081 ccgccggggt gcgtgccaat ctggaggctt tcatcgccct ggcgctggaa ctggacggtg
     4141 ggcgctaccc cagcctgccg cgcttcatcg acgagctggg ccgcttgtcc ggtgcgggcg
     4201 acgacgaggc gccggacgag gggctgatcc ccggcaacgg ggacgatgcg gacgggcgcg
     4261 tgcgcatcat gaccatccat ggcgccaagg gcctggaggc gcccgtcgtg tggctcatcg
     4321 acgcccgcaa cggccaccag aagcccgaca cggatcaggt gctgctcgac tggccgcccg
     4381 gcgacatccg cccgcggcat ttctccctgg ccacccggaa ggacgagcgc ggccggtgcc
     4441 gggcggccct gttcgaggcc gaggagcagg cccgcgcacg ggaggatctg aacctcctct
     4501 acgtggcgat cacccgggcg agccagtatt tcctggccag tggatccctg ccgacccggg
     4561 acgccgccgc gcccaatccg tatcagcgca ttgccgccgc gttggggcgg ctcggggcgg
     4621 aggcgggcac ctggggctct ccgccggtgg aggtcgcggc ggcccctgcg ccggcgcctg
     4681 cagtggcggc tgaggtggcg ccgacaattg cgcccgtgcc tgtcggccag taccgcagcg
     4741 tggccgacgc cgtcgccgaa tcggccggca cactgtttgg aacagccttc cacactgctc
     4801 tggaatgtct cactgaagcg cgctccaccg ccgggctcga ccctgcggcc gtggaactgg
     4861 ccggacgtct cgtcgcccgg ccgcagctgg cgcgtttctt cgacccggcc gcctgtcgct
     4921 gggcgcgcaa cgaagtcgag tttgtggacg ccgctggccg ccttggacgt atcgaccgcc
     4981 tggtggatga cggcgaaacg ctgtgggtgg tggattacaa gaccggtgcg ccggacgagc
     5041 gcctgctgga cgtctaccgg gcccaggtcc ggacctatgg tcgggcggtg gcggcgttgt
     5101 ggccaggacg cccgctgcgc gccgtcctgg tgtttgccgg tggcgaggcg ctcgaggtgg
     5161 ccgtcgacga ggcgggaaat gctgcctgaa tccgtgtcgc agcaggcttc tcgccggctc
     5221 cacaatcgaa taccatcgcg tgcaatacaa aacaaggcgt tcgcgcttct aggggaacaa
     5281 gaacatgctg gtcagtgaaa ttctcgcgat caagggcaag gtgctcttca cgatcgcggc
     5341 caacaagacc ctcgccgatg cggtggcggt gatgacggag caggacgtgg gctcgctggt
     5401 ggtcttcgac aagggccaca tggccggtct gctgaccttc cgcgaagtgc tcgtcgccac
     5461 ccagaaggcc ggcgccggct gggagtccct gccggtcacg gaagccatgc tcaagactcc
     5521 ggtcgtggcc aatcccagca tggaaatgga tgaactgcgc cgccagatgg tcgaacacca
     5581 ccagcgctac ctgccggtga tggacgacac catgctgatg ggcgtggtgt ccttccacga
     5641 cgtggccaag gccgtgctgg aagagcagag cttcgagaac cgcatgctca agaactacat
     5701 ccgcaactgg ccgtcggaag aaggccaggt ctgagcggca ctccggcccg caccgcgcag
     5761 ggggcggacg ggccggcggg atctgcgcat gatcgcggat ccgtcatgac aaggcgcgcc
     5821 cacgcgccga tctggacgta cgttggaagc cggtgccggc aaggcaccgg ggccgcctcg
     5881 ggagggcgga ccgcaggatg accggtcgca ctgcgacggc cgggcccggt ggtcaggctt
     5941 aaaacaacca acgacctaag gagacaacat ggacaagatc tggctgcaaa gctacccccc
     6001 tggcgtgccc cccgaggtgg acctggacga gttctcgtcc ctcgtcgacc tcttcgagca
     6061 gagcgtccgc aagtttgcag cgcgcaccgc ctatgtgaac atgggcaagt ccctcaccta
     6121 tggtgaactg gacgagctgt ccgcgcgctt tgccggctgg ctgcagggcg aactgggcct
     6181 tgggccgggc gcccgcgtcg ccctgatgat gcccaactgc ctgcaatatc cggtggccat
     6241 gttcggcgtg ctgcgggccg gactcacggt ggtgaacgtc aatcccctct ataccgcgcg
     6301 ggagttggaa catcagctca aggacgccgg ctgcgaggcg atcgtcatcg tcgagaactt
     6361 cgcccacacc ctgcaggatg tgctgcccag cacgccggtg cgccacgtgg tgaccaccgg
     6421 gctcggcgat ctgctggggt ttcccaagtc gctgctggtg aatttcgtgg tccgccacgt
     6481 gaagaagatg gtgccggcgt ggagtctgcc cggcagcatc gccttccgcg aggccctggc
     6541 gaagggcgcc acccatcccc tccggccggt gatgctgggg cacgaggatc tggccttcct
     6601 gcaatacacc ggcggcacga cgggcgtggc caagggggcg atgctgaccc acggcaacat
     6661 cgtcgccaac ctgcagcagg cccacgcctg gatcgggcct tatgtgcggg agggcgagga
     6721 gctgatcgtc acggcgctgc ccctctatca catcttctcc ctcacggcga actgcctgac
     6781 cttcttcaag atcggcgccg agaacctgct catcacgaac ccgcgcgaca tcccgggctt
     6841 catcgccgaa ctggcgaagg tccgcttcac cgccatcacc ggcgtcaata cgctgttcaa
     6901 tgcgctgctc aaccatccgg atttcgagaa gctggatttt tcctccctgc ggctggccct
     6961 ggggggcggc atggcggtgc agcaggcggt ggcagagcgc tggaaggcgg tgaccgggcg
     7021 gcccctggcg gaagcctacg ggctcaccga aacgtcgccg gcagtgtgta tcaatcccct
     7081 cgacctgcct gagttcaatc attccatcgg cctgcccatt tcgtcgaccg acgtcagcct
     7141 gcgggacgac gagggctacg aagtcctgtt gggcgagccc ggcgagctgt gcgtcaaggg
     7201 cccccaggtg atgaagggct actacaaccg gccggaggac acggcgcggg tgttcacgcg
     7261 ggacggctac ctgcgcaccg gcgatgtggc ggtaatcgcg cctgacggct acgtgcgcat
     7321 cgtggatcgc aagaaggaca tgatcctggt gtccggcttc aacgtgtatc ccaacgaggt
     7381 ggaagacgtg gtggcgagcc atccgggcgt gctggaggtg gccgccctgg gcgtgccgga
     7441 cgaacgctcg ggcgaggcgg tgaagatctt cgtggtgcgc aaggatccga cgctcaccgc
     7501 ggaggcgctg atcgcccact gccggcaggg cctcaccggc tataaggtgc cccactatgt
     7561 ggagttccgc gaagagctgc ccaagagcaa cgtgggcaag atcctgcgcc gggcgctgcg
     7621 ggacgcgagc acgccgaagt gaacctgcac cgcaccggag cgtggcgcac gccctggtgc
     7681 ggtgcagaaa gcgcttgagt tttgtccgct ttgtgccaca atccggagcc tgaagtttcg
     7741 acctgaaccc ttccgtgacc cataacgccc gtttcctgcc tgctgtcgtc gtagtcgtac
     7801 gcgtagtcag cgtaggcatg gtcgcgtcgt aaggggaaca agcagtcgaa cacgaatttc
     7861 aaacccccgc cggcgcaaaa ccggcggggg tttttgcttt ttggcgcacc ccgaccggga
     7921 agggcagtcc ggcttcccag cctttaggag atcacccgat gatgcagatg accggagcag
     7981 acttgatcat gaagctgctc gaacggcagg gcatccgtac cgtggccggc gtgccgggcg
     8041 gtgcggcgct gccgatgtat gacgccctga cccggagcga atcgatccac cacgtgttgt
     8101 gccgtcacga gcagggcgcc ggtttcatcg cccagggcat ggcgcgggtg tcgggccggc
     8161 ccgaagtctg tctggcctcc agcggtcccg gggcgaccaa cctgattacc gccatcgccg
     8221 atgccaagct ggattccatc cccatcatcg ccctgaccgg ccaggtgccc ctgtccatga
     8281 tcggcaccga tgccttccag gaagtggata cctacggcct gaccatcccc atcaccaagc
     8341 acaacttcct ggtgcgctcg gcgaaggaac tgctcacggt gatccccgcc gccttccgca
     8401 tcgccatgtc cgaccggccc ggcccggtgc tggtggatgt gcccaaggat gtgcagaacc
     8461 agatgatcag cttcgaggac ttccccgacg tggtcgtgcc cgatgcgccg ccgccggtgg
     8521 atatcgccgc cgtggaagaa gccgctcgca tgatcgaggc ggcggagcgt ccggtgctgt
     8581 acctgggcgg cggcgtgatc cactccggcg cgtcgcagct ggccgtgacc ctcgccgagc
     8641 aggcctccct gcccaccacc atgaccctga tggccctcgg cgcgatgccc atggaccatc
     8701 ccctcgccat cggcatgctg ggcatgcacg gggcgcgcta caccaactac gtgctggaag
     8761 agtccgacct gatcatctgc gtcggcgccc gttttgacga ccgggccatc ggcaaggctg
     8821 ccgagttctg ccccaacgcc aagatcatcc acatcgacat cgacccgtct gaactgcaca
     8881 agatcaagac cgcccacgtg gccatcgccg gtgacgtggg gcaggtgctg ggcgagctgc
     8941 tgccgcgggt caagccgcag ctgcgcaagc gctggctgtc ccacgtggag agcctcaagg
     9001 gccgcttccc gctggccatg ccgggcgtgg acgatccgcg cacgccctac ggcctgatcc
     9061 acgcggtggc gagctgtctc gacgacaacg ccatcatcac cagcgacgtg ggccagcacc
     9121 agatgtgggt ggcccaggcc tatcccttcc gccgcgcccg ccagtggctc aattccggtg
     9181 gcctgggcac catgggtttc ggcttcccgg ccgccatcgg cgcagccttg gccgagcccg
     9241 agcgcaccgt ggtgtgcttc tccggcgacg gcagcctgaa gatgaacatc caggaaatgg
     9301 ccaccgccgc ccaggaaaac gtcaatgtga agatcgtgct gttcaacaac cagtccctcg
     9361 gactggtgca tcagcagcag aacctcttct acggcaagcg catcatggcc tcccgctacg
     9421 agcatccgac cgacttcctg aagatcgccg aaggcttcgg catgcgcgcc gtcaatctgg
     9481 atccgtcgcc caacccccgc gcgaccctgg cggaagccct gaacaccccg gggccctgcc
     9541 tgattcacgc gacgatcgac ccgaacgagt tcgtctaccc gatggtgccg ccgggcgccg
     9601 ccaactccga gatgatcggc tgagccccag gagcaaacac gatgtccgac caaacctccc
     9661 aactgtccga gtacatcccc gacgtgcccg agcacaccgg cttccacaag gtcgtgctgg
     9721 agctggccgt ggccaaccac ccgggggtga tgagccacat ctgcaacctc ttcgcgcggc
     9781 gagccttcaa cgtggaaggc atcctgtgca tgccggtggg cgagggcggc accagccgca
     9841 tctggctgct gattttcgag gatgagcgcc tcgagaagat gatccgccag gtcgaaaagc
     9901 tggaagacgt gctctacgtc cgccagcacg ccggtgacca cccggtgttc ggccgcctgg
     9961 acgagttctt ccagtaagta cccgcgtggc cggcgccctg ccggtcgcgt gctagactca
    10021 aggtcggcct gcgggccggc ctttccgttt gttgagacct ctttccgttc gcgagcgccc
    10081 catgtccggc aatacccttg gcaccctgtt caccgtcacg tccttcggcg aatcccacgg
    10141 cccggccatc ggctgtgtgg tggatggctg cccgccgggg ctggccctgt gcgaggcgga
    10201 catccaggcg gagctggacc ggcgcaagcc cgggacgtcc cgtcacgtca cccagcgtcg
    10261 cgagccggac accgtggaga tcctctccgg cgtgttcgaa gggcagacca ccggcacgcc
    10321 gatcgcgttg ctgatccgca accaggatca gcgctccaag gactacggca acatcgccga
    10381 gaccttccgt ccgggccatg ccgactacgc ctactgggaa aagtacggca tccgcgatca
    10441 ccgcggcggc ggccgctcgt cggcgcggga gaccgcggtg cgtgtggcgg ccggcgctgt
    10501 cgcccgcaag tggctggccg agcgctacgg catcgtgatc cgcggctaca tggcccagct
    10561 cgggcccgtg gagattccct tcgcggcctg ggacgatgtg gacgacaacg ccttcttcgc
    10621 gcccaatgcc gccctcgtgc cggcactcga agactacatg gacgagctgc gcaagtccgg
    10681 cgattcggtg ggtgcgcgca tcaatgtggt ggcgaccggc gtgccggtgg gctggggcga
    10741 gccggtgtat gaccgcctcg acgcggacat cgcccacgcc atgatgggca tcaacgccgt
    10801 caagggcgtg gagatcggcg ccggcttcgc cagcgtggcc cagctcggca cccagcattc
    10861 ggacgagatg acgccggagg gcttcctctc caaccaggcc ggcggcgtgc tgggcggcat
    10921 ctcaacgggg caggacatcc tcgtcagcat cgccatcaag cccacctcca gcacccgcct
    10981 ggaccggcgc tccatcaaca aggcgggcga gccggtggtg gtgaataccc acgggcgtca
    11041 cgatccctgc gtgggcatcc gcgccacgcc catcgccgag agcatgctgg ccatcgtgct
    11101 gatggatcat gccctgcgcc atcgtgccca gaacgcggat gtggcctgtc ccacgccgcc
    11161 ggtggcgggt ctggcgcccg acggcgtgca gggcgtgccc tcgcccaagg gttccaaggg
    11221 ttgacgtcca tcaacttttc gggggactca ggggcgtaca gtcggcctca gagcctgtca
    11281 tctacctgaa aacagtccac cgggaggtct tgcatggagc aacacgatct gaatcacgaa
    11341 ttccccgagt accgcgacca gattcattcg ctcaaggtgt cgaacaacca tttctcacgt
    11401 ctgttcgacg aatatcacga cgtgaatcgc cacgtggtcc gggtagagct gaatgccgaa
    11461 ctggcgacgg attttgaact ggaagagctc aagaagcgtc gtctgaagct gaaggatgaa
    11521 ctctacgaca tgctgaaagg cgccgtagta aactgaccgc tgcagctccg ctgaaaaggg
    11581 cgcccgaggg cgctcttttt ttgtcctgcc gccctttgaa tgccccgagc gcccgattct
    11641 tccgacacca caagccctgc ccatgatccc gtactggcgc ctgtcgggct attacttctt
    11701 ctatttcgcc ttcgttggcg ccttctcgcc gtatttcagc ctttacctgc agtccatcgg
    11761 ccgctcggcg gcgcagatcg cgctgctcat gtccctgctg tcggtgatgc gtctggtggc
    11821 gcccaacttg tggggctggc tggcggatcg gctgggcgcg cgggtgccca tcgtgcgcct
    11881 gtcggcggtg ctgagtcttg cgggttttgc gggctttctg atgaccgaat cgccggtcgg
    11941 gctgtttgcg agcatggcgg tgctggcctt tttctggagt gcggctttgc cgctggtgga
    12001 agccttgacc ttttcccacc tgggtgccca cgccagccgc tacggcagca ttcgcgtctg
    12061 gggctcggtg ggcttcattc tcgcggtgct gggcctgggg tatgtgctcg acggtgcgcc
    12121 gatccgcacg ctgctgtgga tcaccctcgg gctgctgggc gggattctgg tctatgcggc
    12181 cgtgctgccg gaaggcgcgg cacccgccca ttcaatgaat ccggcatctc tgggcgaggt
    12241 gctccgccgc agggaagtgc gtgccctgct gaccgcctgt ttcttcatgt cggcggctca
    12301 cggtgcgctg tacgtcttct attcggtcca tctggtgcag ctcggccatc acaagactct
    12361 ggtgggctgg atgtggaccc tcggtgtggt ggcagagatc gccgtgttca tggcgatgcc
    12421 ccagatcctg cggcggtatt ccctgcgggg catcctgctg ttcagctttg ccgtggcggt
    12481 cgtgcgtttc ctgctgatcg gctggggagc caattcattg gccgtgctga tcctggccca
    12541 gatcggtcat ggagccacgt tcggggctta ccatgcggcg ggcgtggcgg cgatcaaccg
    12601 ctggttcggg ccgcaactcc aggcgcgcgg tcaggcgctc tacggcagtg tgtccttcgg
    12661 cgcggggggc atggtgggcg gtgtggtgag tggttacaca tgggacagca tcggcgcagg
    12721 gtggacgtac accctcgggt ccgcgtttgc gttgatgggc ctggcggtcc tgctcgcggg
    12781 ctggcgaccg gcggtccgcg aggcttgaac tcgacaagga gtgggtatgg ctttgatgag
    12841 cgtgtggaag gggatgttgc ccctgggggc agcagtcttg atggtgacgg cctgccagac
    12901 ggtgcagacg acccagtccg gggcggtggg cgtcgaacgg cgccagtcga tgctggtgtc
    12961 gtccagggaa atggagcagg cgtcggcgca gagctatcac aagatcctcc agcaggcggc
    13021 cgagaagaac gccctgaacc gggaccgcgt ccaggtggag cgagtgcgcg tcgtcgcgcg
    13081 tcggctgatt ccggccaccg gcgccttccg ccccgatgcg ccgggctggc gctgggaggt
    13141 caacgtgctc acgtcagagg agctcaacgc ctggtgcatg gccggcggca agatcgcttt
    13201 ctactccggg atcatcgagc gcctgcagct gtccgacgac gaaatggcgg cgatcatggg
    13261 tcacgagatc gcccacgccc tgcgcgagca cgcccgtgag cgggcatccc aggcgatggt
    13321 gaccaacatc ggcgtgagta tcgtcggcgc cgtgctgggt gccgggcagg ccggcaccaa
    13381 cctgatcggg caggtggcca acgtgtcctt cgggctgccc aattcccgcg agcaggagac
    13441 cgaggctgac cgcatcggcg tggagctcgc cgcccgcgcc ggctacgacc cgcgggctgc
    13501 tatcgtgcta tggcagaaga tggccaaggt gtccaacggg gcgccgcccc agtggctgtc
    13561 cacccacccg tccaacgaaa cccgccaggc cgatctgcag cagtacgcgg cgcgggtcat
    13621 gccgttctac gaggaggccc gcaacgcccg acctactcag ggatcgaagc ctgtgaaata
    13681 atcgaaactt ttccgtcgct ttatccgccc atgcacgcgc aaacgctcta cgagaagctc
    13741 tggtcgagcc acgtggtcca cgtcgaggac gacggcaccg ccctgatcta catcgaccgc
    13801 catctggtcc atgaagtcac cagcccgcag gccttcgagg gccttaagct ggccggtcgc
    13861 aagccctggc gcgtgaattc catcgtcgcg acggccgacc acaacacacc taccgaccac
    13921 tgggaccagg gcatccagga tccggtctcg cgccagcagg tggaaaccct cgacgccaac
    13981 atccgcgagg tgggcgcgct ggcctacttc cccttcatgc atgagcgtca gggcatcgtc
    14041 cacgtgatcg gccccgaaaa cggcgccacg ctgccgggca tgaccgtggt gtgcggcgat
    14101 tcccacacct ccacccatgg cgccttcgcc tgtctggccc acggtattgg cacgtccgaa
    14161 gtggagcacg tgatggcgac ccagtgcctg ctgcagaaga agtccaagac cctgctggtg
    14221 cgcgtggatg gcgccctcgg tcccggcgtg accgccaagg acgtggtgct ggccatcatc
    14281 ggtcgcatcg gcaccgccgg tggcaccggc tacgccatgg agtttggcgg cagcgcaatc
    14341 cgcagcctgt ccatggaagg gcgcatgacg gtctgcaata tggccatcga agccggtgcc
    14401 cgcgccgggc tggtggccgt cgacgacaag accatcgcct atctcaagga tcgccccttc
    14461 tcgccccgcg gcgatcagtg ggatcgcgcc gtcacgtatt ggcgcacgct gcactccgac
    14521 gaaggcgcgc agttcgatgc ggtggtggtg ctcaatggcg ccgagatcaa gccgcaggtc
    14581 acctggggca cctcgcccga gatggtcgtg ccggtggatg gcagcgtgcc cgatccggcc
    14641 ctcgaaaagg atccggtcaa gcgcgagggc atggagcggg cgctccagta catgggtctg
    14701 accccgcgca cgccgatcaa caaggtggcg gtggacaagg tgttcatcgg ttcctgcacc
    14761 aattcgcgca tcgaggacct gcgcgccgcg gccgccgtgg ctcgcgggcg tcacaaggcc
    14821 gattccgtcc ggcaggtgct ggtggtgccg ggctccgggc tggtcaagca tcaggccgag
    14881 gcggaaggac tggacaagat cttcatcgcc gcaggcttcg aatggcgtga gccggggtgt
    14941 tccatgtgcc tggccatgaa cgccgaccgc ctcgaaccgg gcgagcgctg tgcgtccacg
    15001 tcaaaccgca atttcgaagg tcgccagggt gccggcggtc ggacgcacct ggtgagtccg
    15061 gccatggcgg cggcggctgc cattgccgga catttcgtcg acgtgcgcaa cctggcctga
    15121 gtcatccaag gagacgacca tgcgtaaatt tgtggcactg atgggcgtgt tcgccgtgct
    15181 gctgctgacc ggctgcaata ccgtgcacgg catcggaaaa gacatcgaaa agggtggcga
    15241 ggccatccag aaggtggcca aataatgaag cccttcaccc gccttgacgg tctcgtggcg
    15301 ccgatggatc gcgccaacgt ggataccgac gcgatcatcc ccaagcagtt cctcaagtcg
    15361 atcaagcgca gcggcttcgg ccccaacctg tttgacgaat ggcgctacct ggacatcggc
    15421 cagcccggca tggacaacac cggccgcccg aagaacccgg agttcgtgct caatcagccc
    15481 cgtttccagg gtgcccagat cctgctcacc cgcgacaact tcggttgcgg cagttcccgc
    15541 gaacacgcgc cctgggcgct ggaggatttc ggcttccgcg tgctgatcgg caccagcttc
    15601 gccgacatct tcttcaacaa ctgcttcaag aacggcctgc tgccgatccg cctgccggcg
    15661 gctgaggtgg atgccctgtt cggccagtgc ctggccacgc ccggctaccg cctcacggtg
    15721 gatctggccc agcagaccgt gacccggccg gatggcaagg ccatcccctt cgaggtggat
    15781 ccgttccgcc gcgaatgcct gctcaacggc tgggacgaca tcggcctgac cctgcgccac
    15841 gccgacaaga tccgcgagtt cgaagctcgc cgccgcatcg agcagccctg gctgttcgcc
    15901 tgagccggcg ccgcgccgga ctttttcacc gccttattct gacgacgcca tgaagattct
    15961 tgttctgccg ggtgacggca tcggtcccga aatcatggag caggccctgc gcgtgctcga
    16021 cgccctgcgc ggggacggtc tgtccttcga ggtcgagcac ggcctgctcg gcggcagcgc
    16081 cgtggatgcc accggcacgc ctttcccgga agccacccgc gccctcgccc gggcggccga
    16141 cgcggtgctc ctcggcgccg tcggcggtcc ccagtgggat gcgctgcccc gcgaccagcg
    16201 ccccgaacgc ggcctgctgg gcatccgcaa ggagctcaac ctcttcgcca acctgcgtcc
    16261 ggccatcctg tacccggaac tcgccaacgc ctccaccctc aagccggaag tcgtggccgg
    16321 gctggacatc ctcatcgtgc gcgagctgac cggcgacatc tacttcggcc agccccgcgg
    16381 cgtgcgggaa gagaacggcg agcgggtcgg cttcaacacc atggtgtatt ccgagtccga
    16441 gatccggcgc atcggcaagg tggccttcga ggccgcccgc aagcgcgaca agcgcctgtg
    16501 ctcggtggac aagatgaacg tgctggaatg cacccagctg tggcgcgacg tgatgatcga
    16561 gctgtcggcc gactacccgg acgtggcgct ctcccacatg ctggtggaca acgccgccat
    16621 gcagctggtc cgcgcgccca agcagttcga cgtgatggtc accggcaaca tgttcggcga
    16681 catcctgtcc gatgaggcgt ccatgctcac cggctccatc ggcatgctcc cctccgcgtc
    16741 cctggatcag aacaacaagg gcctttacga gcccagccac ggctcggcgc cggacattgc
    16801 cggcaagggt gtcgctaatc cgctggccac catcctttcg gcagcgatga tgctgcgcta
    16861 taccttcaat ctggaggctc ccgcggcgcg catcgagtcc gcggtcaaga aagtgctggc
    16921 ggccggctac cgcaccggcg acatctacga ggcgggtacc cgccgggtgg gcacccagga
    16981 aatgggcgac gcggtcatcg cggcgctgtg a
//
LOCUS       DAOQPA010000080        16809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1714_length_16809_cov_16.340635, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000080 DAOQPA010000000
VERSION     DAOQPA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_16809_cov_16.340635"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="fliW"
                     /locus_tag="PKX01_11520"
     CDS             complement(<1..413)
                     /gene="fliW"
                     /locus_tag="PKX01_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HMZ76801.1"
                     /translation="MKITSPMLGEVDVSPERILDFPTGLPGFEGNTRFTLLHAEGDAP
                     KVFLLQSLDDTAVAFSVTTPDVLGLNYEFTLSDDEVAALQLASADDACVLLIVSRDED
                     TTAPLRASLMAPVVLNTTTRRGLQKIIGKVDTSVTL"
     gene            complement(617..931)
                     /locus_tag="PKX01_11525"
     CDS             complement(617..931)
                     /locus_tag="PKX01_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76802.1"
                     /translation="MYHVTVAFRADREYEFHVHGDEVAGLDTEAARDWLQQEFDALEC
                     TPSNPVGKILVLDVILNVAKYGGESRFAQGGAWARRFAACTGVVLDRPAIRVDVPGFT
                     VA"
     gene            1082..2353
                     /locus_tag="PKX01_11530"
     CDS             1082..2353
                     /locus_tag="PKX01_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HMZ76803.1"
                     /translation="MTVSPWRALANRRMLICVLTGFSSGLPLYLLLNLVPAWLKTEGL
                     SLKAIGAFALIQFPYTWKFLWSPLLDRFALPLLGRRRGWMAATQLALLAAVAWLGTLT
                     PGGQLGLVVGLTALIAFLSATLDIVLDAYRRELLPEIELGIGNAIHVNAYKLAGLVPG
                     SLSLVLADHLPWAQVFLVTSLFMLPGLLMTLLVGEPVAAAAAPRTLRDAVVAPFREFF
                     GRHGVGAALWVLAFIFLYKLGDSLCTALATPFYLDMGFSKTEIGLVAKNAGLWPSVIG
                     GLLGGLWMVRLGINRALWVFGFVQWAAIFGFAWLAWLGPAEADAARLGVLALVIGLEA
                     LAVGLGTTAFVAYMARTTHPAYTATQLALFTSLMAVPRTFINASAGWLVEDLGWFRFF
                     LLCGLLAVPGMLLLFRVAPWRLADDSSEGMK"
     gene            2350..2940
                     /locus_tag="PKX01_11535"
     CDS             2350..2940
                     /locus_tag="PKX01_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMZ76804.1"
                     /translation="MMGKGRLEAFSDGVLAIIITIMVLELKVPHGDDLAALAPLLPVF
                     LSYLLSFVYVGIYWNNHHHLLHAAHGVTGAVLWANLHLLFWLSLIPFVTGWMGENHFS
                     ALPTALYGGVLLMAAIAYFILQWAILRGDGTASVLAQALGRDWKGKLSPVIYIVAIPT
                     AFFAEWVSDALYVLVALIWLVPDRRIERALVVDEHP"
     gene            2970..3683
                     /locus_tag="PKX01_11540"
     CDS             2970..3683
                     /locus_tag="PKX01_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ76805.1"
                     /translation="MTALEAAARELSAALAPMRFAAPVSHVYNPLAYAWAPYADYLRR
                     FGQGRKRVVLVGMNPGPFGMVQTGVPFGEVAAVRDWMGIRAEVTAPAAQNPKRPVEGF
                     ACTRSEVSGRRLWGLFAERFGPAEAFFAEHFVANYCPLAFFDGGRNLTPDKLPAAEQG
                     PLLAACDAHLRRVAESLQPEWVIGVGAWAELRAREALAGLPLRFGRVLHPSPASPAAN
                     RGWAEAATRQLVDLGVWPA"
     gene            complement(3691..5175)
                     /locus_tag="PKX01_11545"
     CDS             complement(3691..5175)
                     /locus_tag="PKX01_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HMZ76806.1"
                     /translation="MRTLENLPFDNAFARLPEQFYTRLAPDPLPEPVLVAASPAAAAL
                     IDLDPAEFARPEFARVFAGNALLPGSEPLAAVYSGHQFGMWAGQLGDGRAHLLGEVPG
                     AGAGWEIQLKGAGQTPYSRFADGRAVLRSSLREFLCSEAMAALGIPTTRALCVVGSPL
                     PVRRETVETAAVCTRLAPSFLRFGSFEHWAVNKQPDALRVLADYVIDRFRPELRDAAN
                     PYDALLRDAGRRTGDLIARWQTVGFMHGVMNTDNMSILGLTLDYGPFGFMDGFDANHI
                     CNHSDDHGRYAYKAQPQIGHWNLFALGRALRSLVGSQDDVKAAIADSYVPAFENGYLN
                     GLRAKLGLQDALPGDEDFIGETLSLLQRFHPDYTTFFRVLSRLPGAVTDASRATVDAP
                     LRDHFIDRDACDAWLTLWRARLAAEGSRDDVRQAAMLTVNPKYVLRNWVAEEAIRAAQ
                     QGDDRVTAEVLACLSRPFDEQPEFERYAAPPPDWAQHLAVSCSS"
     gene            5324..6748
                     /locus_tag="PKX01_11550"
     CDS             5324..6748
                     /locus_tag="PKX01_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ76807.1"
                     /translation="MLQTHIDAPADAGRPAAPLPDSGVDLKRYLLWRVSLFALAIGLA
                     AVAAVFVQTGERLRGHIARTGSTVENLLAAEVDRPRDSFRRTVEGLDLTSLDGIGPLL
                     GICVKVEDIYKRAVIRRCFDEGEGAPAPVRAVLAALLGPDVAYRGVIGQYPGVKVGEF
                     TVTPDLDAAARDAWSRIGMVLGMTFGILLLNGLVVLPVRRALAPAEQILAVLARMEAG
                     DLAARMPRPRLIELRRIAAGFDHLVERLEATLAARRQLAQRLLAVREEERRHLARELH
                     DEFGQCLASVSAEAAFIGARAERSDPSLLPAARAVSAVTAHMMESLQGILHQLRPVGL
                     EEFGLAAALEQLVGGWRRRQPDCQFHLEMRGAVDDLPDELTVSLYRIVQESLTNALRH
                     GAPRRVAVSLVRDADGCRVTVDNDGQGRTEPNPGSGLGVLGMRERVEALGGQFDLLPL
                     TPQGMRVAAVLPVAALIPVETSHA"
     gene            6741..7376
                     /locus_tag="PKX01_11555"
     CDS             6741..7376
                     /locus_tag="PKX01_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76808.1"
                     /translation="MLEPTRLLLVDDHAVVREGYRRLLETRAELRIVGEAATARDALD
                     AFRRLAPDVLVLDLGLPDMGGVELIRRLLQREPGARILVFTMHREPLFATQALRAGAQ
                     GYVTKSSPPEVLVQAVLQVAAGRPFISPDLAPELALALVDRPRAPLADLAPREFEILR
                     LLLEGLDPEEIGRRLSLSAKTVRNCHYQIKSKLGVRSDIELTRLAMAHGLV"
     gene            complement(7433..7876)
                     /locus_tag="PKX01_11560"
     CDS             complement(7433..7876)
                     /locus_tag="PKX01_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HMZ76809.1"
                     /translation="MHTFDERSIPWQPFGDLPHFVFAIQHVDRAHRTADVLFKFAAGQ
                     QIVLHRHKALNHTFVVQGEHRLYEPDGRLREVRPAGTYTVTPASETPHREGGGATDAV
                     VLFSMRPAPGEILYELLDDDGRVIAELTLETLADLFDAARAPAVA"
     gene            8137..9099
                     /locus_tag="PKX01_11565"
     CDS             8137..9099
                     /locus_tag="PKX01_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76810.1"
                     /translation="MNLHSGATLLALTLLAGTAAADNRFCGGAVVQSGEPTDLAHVVG
                     DQTLEFVADQPASLTVCSKGYLLEKCGDHETALKIFDRCIAAGYVGAMIWKALMLQDG
                     AGTPPDLPAAAELMRRAAHSPDSPYATLGKLHYASALHEGKGVPRDEVAARRWFEAAA
                     ADGNPDAQEFLRTGYHVGDRDLAGAGTGVAPPVPAPAGTPSQEGEPPRLLASTGARGD
                     GLRSAASTSLAARRLGPPAASPPPISELPVVPALLREDALATEVPPDVRGQRLARVEA
                     AAPEAPGSAVSRWLPVALLGTVLAGALGQGRGRGALPAVAGSVA"
     gene            9096..9587
                     /locus_tag="PKX01_11570"
     CDS             9096..9587
                     /locus_tag="PKX01_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ76811.1"
                     /translation="MNAALESLLYEASQLFLVPVLLAIAGLFLYAFYAVGAFLWQARQ
                     RHAGDPAGFELMGLLAAQPDLGVADLEAHAFRRLEVARIATRVAPMLGLVATMIPLGP
                     ALRALGDGHMADVSRGLGVAFSAVILALVAASLTFWVVNVRRRWYASDLLAIEARLSG
                     SGR"
     gene            9584..9901
                     /locus_tag="PKX01_11575"
     CDS             9584..9901
                     /locus_tag="PKX01_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HMZ76812.1"
                     /translation="MRLRMHLGEAEDDDPVLSLVNLIDVFLVIVAALLLAVAQNPLGP
                     FAEGKVTVIRGAGTPQMEVVIKDGAKIERFRGDGGAASASGGVRAGTAYRLADGSLVY
                     VPE"
     gene            complement(9905..11986)
                     /locus_tag="PKX01_11580"
     CDS             complement(9905..11986)
                     /locus_tag="PKX01_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76813.1"
                     /translation="MNTPRHLPILNLRQVEGHPDRLCGTVRMPDADRDTHLLVLRRGQ
                     HWSAVPAHCPHEGHRLDQSALDADGRLICPAHGLEVDTQGSRNILPVRRAGDEFIIEL
                     PAADALQLTPVADVQRLQAEVEALRQANAALEQQIGAVTATMDAMVDDVSFQSRELAA
                     RSAEQTRLSAFVTNVMETMDSLLLVLDRFGAIRQANAALRHRLGIDPAGLSGQSPDPL
                     LAPETLTTLAADLPSAGAGTLLFRTILKAGSLEMETGMPSRLAGDPVRHFILRATTLY
                     DRSGKLDGVVIVGSDITALRAREQALRESEQLFRDYSALSTDWFWQTDADMRFTTYVG
                     PPRGGHDLGVVVAGKRREDFADPEDLRNAVKWQAYHAEIDARRQFRDFEYRVVHPLIG
                     TTWFSVSGMPLFAPDGAFLGYRGTAKDITGPKAIEKELRAHRDHLSELVAAQTADLVS
                     AKETAERANQLKSEFLANMSHEFRTPLHGILSYARLGLTRLGQVPPDKIAAYFNRIHQ
                     SGSRLAGLVNDLLDLSKLEARRVDFRLQAADVAVVVERVRGDLAALIASRGLSVAVER
                     ATVSTVAMVDTQQLYHAIQNLLSNAIKFSPDGGVLTLRFADALLGEQDALAFQVRDRG
                     VGIPPGEEERIFDKFIQSSATKTGAGGTGLGLAITREIVRGHQGLIEARNHPDGGAVF
                     EIRLPRRAAED"
     gene            complement(11983..12444)
                     /locus_tag="PKX01_11585"
     CDS             complement(11983..12444)
                     /locus_tag="PKX01_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HMZ76814.1"
                     /translation="MASEQTHIICFGNALHGDDGFGPTVYTHLGETPLPPGVRLFCAD
                     TAGLGALACFEDCTTAIVVDALRGFGPPGSLHSLAAGELEGSDDAASANIHGAGVSSL
                     LRLLPVALPTPPRVRVIGVEIATLSPFQPGLSAAVAARLPEALAHIRRLLA"
     gene            complement(12434..13969)
                     /locus_tag="PKX01_11590"
     CDS             complement(12434..13969)
                     /locus_tag="PKX01_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMZ76815.1"
                     /translation="MPLTTLNIDLNRVEGDLEFQVDLDGDTVVDARCVGTLFRGFEQL
                     LIGRAPRDALVITPRVCGICGTAHLYTAALALERLAQIPVSPHATLVRNLCLMAENVQ
                     SDLRQSFLFFTPDLVHRRYADEPLFADVERAFAPLKGRIVRGALGATRKILELVAIFG
                     GQWPHSSYMLPGGITRGATARDLVDCHDIVAQAREWYEQDVIGDTLEHWLALDSADAF
                     FAWLDTAAPAGSALGLLTQFARSIGLQHIGAGAGHFLSAGSWHDPQRWQPPYGEAAGL
                     IEAGLYDGQHGTVTPFDPALINEHVRHAWYRPYPGGRHPYDGETVPDYQPDSDRYTWA
                     KAPRYGDAVVETGPLAELLVGGDRLIRDLLRQEGPNTWLRQFARLRRAGLTLHLMKRH
                     LDDLAGQLGTPHFLPPPDNALADGEGFGLVQAARGTLGHWIRVEQGRISRYQIITPTA
                     WNASPKDSNGRHGHWEQSVIGVRLADPGDPVEIGHIIRAHDPCLVCTVHVVGRGTRHR
                     YGV"
     gene            complement(13985..14926)
                     /locus_tag="PKX01_11595"
     CDS             complement(13985..14926)
                     /locus_tag="PKX01_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HMZ76816.1"
                     /translation="MATLIWLQTGACSGDTLSLLNADSPSIERLTADGTLDILWHPSL
                     TAGPPGHLGTLIAAVEAGAQPLDLLCVEGSLITGPYGSGMFDSYRGRPKIRIVEALAA
                     RARVVVAIGTCAAFGGIPAAPPNPTDCTGLQFDRESPGGLLSPEWRSSAGLPVINLAG
                     CPTHPTTITRALAMLARDQPLELDFLNRPRLFFGAMVHQGCTRNEYHEYDVEETQPGG
                     RGCMYFNLGCQGPSTLATCNSELWNRRSSKTRAGVPCFGCTAPGFPREGDLFRTEKIG
                     AVPVTLPLGVSRAHYVAYKNLAQAAAPLRVKNREMEP"
     gene            complement(15068..15481)
                     /locus_tag="PKX01_11600"
     CDS             complement(15068..15481)
                     /locus_tag="PKX01_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76817.1"
                     /translation="MSPDDGLLDALRLRISPEAGLFLGSLRESFGPLWLLYDGQAPDE
                     RERLRWAPALREPPPEGYSPWACCACGTLLVTDADRSPLAQACLDVDLVEQKPGSTTL
                     SCTGLYLSAHLWPLGGTDLALRRALEAEQRSTAGR"
     gene            complement(15503..15874)
                     /locus_tag="PKX01_11605"
     CDS             complement(15503..15874)
                     /locus_tag="PKX01_11605"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HMZ76818.1"
                     /translation="MTERIVATEAAVQFIDELRRDFGPVIFLLSGGCCDGSVPNCFKV
                     GEFMPSQTTLLLGTVGGAEFWLGHQEYIYYENSQLTLDVRPGQGGDFSLDCGRGRAFE
                     TTSRPFTDAELATVLPPVRGA"
     gene            complement(15969..>16809)
                     /locus_tag="PKX01_11610"
     CDS             complement(15969..>16809)
                     /locus_tag="PKX01_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ76819.1"
                     /translation="ATSKRIAKIAFTGSTATGKIIGQAAASNLIPATLELGGKSPNIF
                     FADVMDKDDGFLDKAIEGMVLFAFNQGEVCTCPSRALIQESIYDKFIERVLPRVAAIK
                     QGSPLDTDTMIGAQASQMQIDKITEYLAVGKQEGAQCLIGGERAHLSGDLSEGYYIQP
                     TLFKGHNKMRIFQEEIFGPVLAVTTFKDEAEAVAIANDTVYGLGSGVWSRNGNVAYRM
                     GRAIKAGRVWTNCYHAYPAHAAFGGYKESGIGRETHHMMLDHYQQTKNLLVSYAEQKL
                     GFF"
BASE COUNT         2312 a         5379 c         6419 g         2699 t
ORIGIN      
        1 agggtgacgc tggtgtccac cttgccgatg atcttctgca ggccgcggcg ggtggtggtg
       61 ttgagcacca ccggcgccat caggctggcc cgcagggggg ccgtcgtgtc ttcgtcgcgg
      121 ctgacgatga ggagcacgca ggcatcgtcg gcgctggcca gttgcagggc ggcgacctcg
      181 tcgtcgctga gggtgaattc gtagttgagg cccagcacat cgggcgtggt gaccgagaag
      241 gccacggcgg tgtcgtccag gctctgcagc aggaagacct tgggggcgtc gccctcggcg
      301 tggagcaggg tgaagcgggt attgccctcg aagccgggca ggccggtcgg gaaatcgagg
      361 atgcgttcgg gcgacacgtc cacttcgccg agcatggggc tggtgatctt catggtttcc
      421 ctccggttcg gtcggttggc ccggcgcggc cgggcgatcc gggcgtcggg ccgcggatct
      481 tcagcagaca cgatagcgcc ggcggcggac ggcccaccgc ggaaagagag gcgggaatcg
      541 cccccaattc tccccgtttt gtagaggggc tttggccagg gcgacgcgct tgcccgatgc
      601 cccggcgggc ccggattcag gcgaccgtga agccgggcac gtccacgcgg atcgccggcc
      661 ggtcgaggac cacgccggtg cacgcggcaa agcgccgcgc ccacgcgccg ccctgggcga
      721 agcgggattc gccaccgtac ttggccacgt tgaggatcac gtcgaggacc aggatcttgc
      781 ccaccgggtt gctgggcgtg cattcgaggg cgtcgaattc ctgctgcagc cagtcccgcg
      841 cggcctcggt gtcgaggccg gcgacttcgt ccccgtggac gtggaattcg tattcccggt
      901 cggcccggaa cgcgacggtg acgtggtaca tgcgggtctc ctcgttgtat tctggcggcg
      961 tccggacgcg cctggcggcg tggcggacgc tgcatttttc tgtggccggg tgccggaatc
     1021 aagtaaccga ttgttatcat ccggcgctcc cccgcctgac cccgccctgc gttccgaatc
     1081 catgaccgtt tccccatggc gcgccctggc caatcggcgc atgctgatct gcgtgctcac
     1141 cggcttctcg tcgggcctgc ccctgtacct gctgctcaat ctggtgccgg cgtggctgaa
     1201 gaccgagggg ctgtccctga aggccatcgg cgccttcgcg ctgatccagt tcccctacac
     1261 ctggaagttc ctgtggtcgc cgctgctgga ccgcttcgcc ctgcccctgc tgggccggcg
     1321 gcggggctgg atggcggcga cgcagctggc cctgctggcg gcggtggcgt ggctcgggac
     1381 gctcacgccg ggcggacagc tcggcctggt ggtgggtctg acggcgctga tcgccttcct
     1441 gtcggcgacc ctcgacatcg tcctggatgc ctaccggcgc gagctgctgc cggagatcga
     1501 gctgggcatc ggcaacgcca tccacgtgaa cgcctacaag ctggccggcc tggtgccggg
     1561 cagcctgtcc ctggtgctgg cggatcatct gccctgggcg caggtcttcc tggtgaccag
     1621 cctgttcatg ctgccgggcc tgctgatgac cctgctggtg ggtgagccgg tggccgcggc
     1681 ggccgcgccg cgcaccctgc gggacgcggt ggtggcgccc ttccgcgagt tcttcggccg
     1741 tcacggcgtg ggcgccgccc tgtgggtgct ggccttcatc ttcctctaca agctcggcga
     1801 ttccctgtgt accgcgctgg ccacgccttt ctacctggac atgggcttct ccaagaccga
     1861 gatcggcctc gttgccaaga acgccggcct gtggcccagc gtcatcggcg gcctgctggg
     1921 cgggctgtgg atggtccggc tgggcatcaa ccgcgccctg tgggtgttcg gtttcgtgca
     1981 gtgggcggcg atcttcgggt ttgcgtggct cgcctggctg ggaccggccg aggccgacgc
     2041 ggcgcgcctg ggcgtactgg ccctggtgat cgggctggaa gcgctggcgg tgggactcgg
     2101 caccaccgcc tttgtcgcct acatggcgcg caccacccat cccgcctaca cggccacgca
     2161 gctggccctg ttcaccagcc tgatggcggt gccgcgcacc ttcatcaacg cctccgccgg
     2221 ctggctggtg gaggatctgg gctggttcag gttcttcctc ctgtgcggct tgctcgcagt
     2281 gcccggcatg ctgctgctgt ttagggtcgc cccgtggcgc ctggcggatg attccagtga
     2341 agggatgaaa tgatgggaaa aggacgcctg gaggccttca gtgacggcgt gttggcgatc
     2401 atcatcacga tcatggtgct tgagctcaag gtcccccatg gcgatgacct ggccgcgctg
     2461 gccccgctgc tgccagtgtt tctcagctac ctgctgagct tcgtctatgt cggcatctac
     2521 tggaacaacc accatcacct cctgcatgca gcccacgggg tgacgggggc ggtgctgtgg
     2581 gccaacctgc atctgttgtt ctggctgtcg ttgattccct ttgtcacggg gtggatgggc
     2641 gagaatcact tttccgcctt gccgacggcg ctctatggcg gcgtgctgtt gatggcggcg
     2701 atcgcatact tcatcctgca gtgggcgatc ctgcggggcg atggcactgc ctcggtgctg
     2761 gcccaggcgc tgggccgcga ctggaagggc aaactgtcgc cggtgatcta catcgtcgcg
     2821 attcctacag ccttctttgc cgaatgggtc tccgacgcgc tgtacgtgct ggtggcactg
     2881 atctggctgg tgccggaccg gcgcatcgag cgggcccttg tcgtcgacga gcacccctga
     2941 atgcctgctt tgatggatct ccccacgcca tgactgccct cgaagccgcc gcccgcgaac
     3001 tctccgccgc cctcgcgccg atgcgcttcg cggcgcccgt cagtcacgtt tacaaccctt
     3061 tggcgtacgc ctgggcgccc tacgcggact acctgcggcg cttcggccag gggcgcaagc
     3121 gggtggtgct ggtgggcatg aaccccggcc ccttcggcat ggtgcagacc ggcgtgccct
     3181 tcggcgaggt ggccgcggtg cgggactgga tgggcatccg tgccgaggtg acggcgccgg
     3241 ccgcgcagaa ccccaagcgg cccgtggagg gcttcgcctg cacccgctcg gaggtgagcg
     3301 ggcggcgcct gtgggggctg ttcgccgagc gcttcggccc ggcggaggcc ttcttcgccg
     3361 agcacttcgt ggccaactac tgcccgctgg ccttcttcga tggcgggcgc aacctcacgc
     3421 cggacaagct gcccgccgcc gagcagggac cgctgctggc agcctgcgac gcgcatctgc
     3481 ggcgggtggc ggagagcctc cagccggaat gggtgatcgg cgtgggcgcg tgggccgagc
     3541 tgcgcgcccg cgaggcgctg gcgggtctgc ccctgcgctt cgggcgggtg ctccatccca
     3601 gcccggccag cccggcggcg aatcggggct gggccgaggc ggcgacgcgc cagctggtgg
     3661 acctgggcgt gtggccggcc tgaggggcgc tcagctggag cagctcaccg ccagatgctg
     3721 ggcccagtcc ggcggcgggg cggcgtagcg ttcgaattcg ggctgctcgt cgaagggccg
     3781 cgacaggcag gcgagcacct cggcggtgac gcggtcatcg ccctgctggg cggcgcggat
     3841 ggcctcctcg gcgacccagt tgcgcagcac gtacttggga ttgacggtga gcatcgccgc
     3901 ctggcggacg tcgtcgcggc tgccttcggc ggcgaggcgc gcgcgccaca gggtgagcca
     3961 ggcgtcgcag gcgtcccggt cgatgaagtg atcgcgcagg ggcgcgtcca cggtggcgcg
     4021 gctggcgtcg gtgaccgcgc cgggcaggcg cgacaggacg cggaagaagg tggtgtagtc
     4081 cgggtggaag cgttgcagca gggacagggt ctcgccgatg aagtcctcgt caccgggcag
     4141 ggcgtcctgc aggccgagct tggcgcgcag gccgtttagg tagccgttct cgaacgccgg
     4201 cacgtagctg tcggcgatgg cggccttcac gtcgtcctgg ctgcccacca ggctgcgcag
     4261 ggcgcggccg agggcgaaga ggttccagtg gccgatctgg ggctgggcct tgtaggcgta
     4321 gcggccgtgg tcgtccgagt ggttgcagat gtggttggcg tcgaagccgt ccatgaagcc
     4381 gaaggggccg taatccaggg tgaggcccag aatggacatg ttgtcggtgt tcatcacccc
     4441 gtgcatgaag cccaccgtct gccaacgggc gatcaggtcg ccggtgcggc ggccggcgtc
     4501 ccgcagcagg gcgtcgtagg ggttggcggc gtcgcgcagt tcgggacgga agcgatcgat
     4561 gacgtagtcg gcgaggacgc ggagggcgtc cggctgcttg ttcaccgccc agtgctcgaa
     4621 ggagccgaag cgcaggaagc tgggcgccag gcgggtgcac accgcggcgg tctcgacggt
     4681 ctcgcggcgc acgggcaggg gcgagcccac cacgcacagg gcgcgggtgg tggggatgcc
     4741 gagggccgcc atggcttcgg agcagaggaa ttcccgcagg ctggagcgca gcaccgcgcg
     4801 gccgtcggca aagcgcgagt agggcgtctg gccggcgccc ttgagctgga tctcccagcc
     4861 ggcgccggcg cccggcacct cgcccagcag atgggcgcgg ccgtcgccga gctggccggc
     4921 ccacatgccg aactggtggc cggaatacac cgcggccaag ggctcgctgc cgggcagcag
     4981 ggcgttgccg gcaaataccc gggcgaactc cgggcgggcg aattcggccg ggtccaggtc
     5041 gatcagcgcg gcggcggcgg ggctggccgc caccagcacg ggctcgggca gcgggtccgg
     5101 cgcaaggcgg gtgtagaact gctcgggcag gcgcgcgaac gcgttgtcga agggcaggtt
     5161 ttcaagggtg cgcatggagg gctccggtgg cggcgcatcg ggcaaattgc ccacgcgctc
     5221 cgcggtaaag tgccggcagg cgggacggcg gtccggccta aaatccgact atttcactat
     5281 ggtatggttc cgcggccccc acgggaagcg ggccgacgca aaaatgctgc aaacgcacat
     5341 cgacgcgccc gcggatgccg ggcggcccgc cgccccgttg cccgattccg gcgtggatct
     5401 gaagcggtat ctcctgtggc gggtgagcct gttcgccctg gccatcggcc tcgcggcggt
     5461 ggcggcggtc ttcgtgcaga ccggcgagcg cctgcgcggc cacatcgccc gcaccggcag
     5521 cacggtcgag aatctcctcg ccgccgaggt ggatcgtccg cgggacagct tccggcgcac
     5581 cgtggagggc ctcgacctca cctcgctgga cggcatcggc ccgctgctgg gcatctgcgt
     5641 gaaggtggag gacatctaca agcgggcggt gatccggcgc tgtttcgacg agggcgaggg
     5701 cgcgcctgcc ccagtgcggg ccgtgctggc cgccctgctg gggcccgacg tcgcctaccg
     5761 gggggtgatc ggccagtatc ccggcgtgaa ggtgggcgaa ttcaccgtga cgccggacct
     5821 ggacgccgcc gcgcgggacg cctggagccg catcggcatg gtgctcggga tgactttcgg
     5881 catcctcctc ctcaacgggc tggtggtgtt gcccgtgcgc cgcgccctgg cgccggccga
     5941 gcagatcctg gcggtgctgg cgcgcatgga ggcgggcgac ctggccgcgc gcatgccgcg
     6001 cccgcggctg atcgagctgc gccgcatcgc ggcgggcttc gaccacctgg tggaacgcct
     6061 ggaagccacc ctcgccgccc gccgccagct tgcccagcgt ctgctggcgg tgcgggagga
     6121 ggagcgccgg cacctggcgc gggagctgca cgacgaattt ggccagtgtc tcgcctcggt
     6181 gagcgccgag gcggccttca tcggcgcccg cgccgagcgc agcgatccgt ccctgctgcc
     6241 cgccgcccgc gccgtgtcgg cggtgaccgc ccacatgatg gagagcctgc agggcatcct
     6301 gcaccagttg cgaccagtgg gtctggagga gttcggcctc gccgccgctc tggagcagct
     6361 ggtgggcggc tggcggcggc gtcagccgga ctgtcagttt catctggaga tgcgcggtgc
     6421 ggtggacgat ctgcctgacg agctgaccgt gagcctctac cgcatcgtgc aggaaagcct
     6481 caccaacgcc ctgcgccacg gcgcgccgcg gcgcgtggcg gtcagtctgg tgcgggatgc
     6541 ggacggctgc cgggtgaccg tggacaacga tggccagggc cgcaccgaac cgaacccggg
     6601 cagcggcctg ggcgtgctgg gcatgcgcga acgggtcgag gccttgggcg ggcagttcga
     6661 cctgctgccg cttacgcccc agggcatgcg cgtggcggca gtgctgccgg tcgcggctct
     6721 gatccctgtg gagacatccc atgcttgaac cgacccgcct gctgctggtg gacgaccacg
     6781 cggtggtccg cgaaggctat cgccggctgc tggaaacccg cgccgagctt cgtatcgtcg
     6841 gcgaggcggc caccgcccgc gacgccctcg acgccttccg ccgcctggcg ccggacgtgc
     6901 tggtgctgga cctgggcctg cccgacatgg gcggcgtgga gctgatccgg cgcctgctcc
     6961 agcgggaacc cggcgcgcgc atcctggtgt tcacgatgca tcgcgaacca ctcttcgcca
     7021 cccaggccct gcgcgccggc gcgcagggct atgtgaccaa gagcagtccg ccggaggtgc
     7081 tggtgcaggc cgtgcttcag gtggcggccg ggcggccctt catcagccct gacctggcgc
     7141 cggagctggc cctggccctg gtggaccggc cgcgggcgcc cctcgccgac ctggcgccgc
     7201 gggagttcga gatcctgcgc ctgctgctgg aggggctgga cccggaggag atcggccggc
     7261 gcctgtccct gtcggccaag acggtgcgca actgccacta tcagatcaag agcaagttgg
     7321 gcgtgcgcag cgacatcgag ctgacccgcc tggcgatggc ccacgggctg gtgtgagggc
     7381 gcccggcgca cggccgcggg cgtgggcacg gtccggcggg gcgcaggagg cttcaggcga
     7441 cggcgggtgc gcgggcggcg tcaaagagat cggcaagggt ctccagggtg agctcggcaa
     7501 tcacgcggcc gtcgtcgtcg aggagttcgt agaggatttc gccgggtgcc gggcgcatgc
     7561 tgaagagtac gacggcgtcc gtcgcgccgc cgccttcccg gtggggcgtc tcgctggcgg
     7621 gcgtgacggt gtaggtgccg gcggggcgga cttcccgcag gcggccgtcg ggctcgtaga
     7681 ggcggtgctc gccctgcacg acgaaggtgt ggttgagggc cttgtggcgg tgcaggacga
     7741 tctgctggcc ggcggcgaac ttgaacagca cgtcggccgt gcggtgcgcc cgatccacgt
     7801 gctggatggc gaacacgaag tggggcagat caccgaaggg ctgccagggg atggaacgtt
     7861 cgtcaaaagt gtgcatggag gtctccggaa ggacggggcg gtgaatgagc ccgggcggac
     7921 gccgcccggt gccggcattg gacgcccgga tcggcctcac cgccgagggc cgcgtgcccg
     7981 cgggacaacg ggcatccggc ccgcccgggg gcagatgcga tggcgggcaa aatgcccgcc
     8041 cgtgcggggc gaaatgccgc tgacgccggt ccggtcgatg cctacagtgg ggggcgaagc
     8101 gggccgctgg cctgcattgc ctggacgggg acagccatga acctccactc cggcgccacg
     8161 ttgctggcgc tgaccctgct ggccggcacg gccgcggcgg acaatcgctt ctgcggcggc
     8221 gccgtggtcc agagcggcga gccgaccgac ctggcccacg tggtgggcga ccagacgctg
     8281 gaattcgtgg ccgaccagcc ggccagcctg acggtgtgca gcaagggcta cctgctggaa
     8341 aagtgcggcg atcacgagac ggcgctgaag atcttcgacc gctgcatcgc cgccggctac
     8401 gtgggggcga tgatctggaa ggcgctgatg ctccaggacg gggccggcac gccgccggat
     8461 ctgcccgccg ccgccgagct gatgcgccgg gcggcccaca gcccggattc gccctacgcg
     8521 accctgggca agctgcacta cgccagcgcc ctgcatgagg gcaagggcgt gccgcgggac
     8581 gaggtggcgg cacggcgctg gttcgaggcc gcggcggcag atggcaaccc ggatgctcag
     8641 gagttcctgc gcaccggcta ccacgtgggc gaccgggatc tggccggcgc cggcaccggc
     8701 gtggcgccgc ccgttccggc acccgcgggg accccgtcgc aggagggcga accgccgcgc
     8761 ctgctcgcgt cgaccggggc gcgtggcgac ggcctccgat cggcggcgtc cacgtccttg
     8821 gcggcgcgcc gtctcggacc acccgccgcg tcgccgccgc ccataagcga actgccggtg
     8881 gtgccggcgc tgctgcgcga agacgccctg gcgaccgagg tgccgccgga tgtccgcggc
     8941 cagcgtctgg cgcgggtgga ggctgccgca ccggaggcgc ccgggagcgc cgtgtcgcgc
     9001 tggctgccgg tggctctgct ggggacggtg ctcgcggggg cgctggggca ggggcgcgga
     9061 cgcggagcgc tgcccgccgt ggccgggagc gtcgcatgaa cgcggcgctg gaatccctgc
     9121 tctacgaggc gagccagctc ttcctggtgc cggtgctgct ggccatcgcc ggactgttcc
     9181 tgtatgcctt ctacgcggtc ggcgcctttc tgtggcaggc ccgccagcgt cacgccggcg
     9241 atcccgccgg gttcgagctc atgggcctgc tggcggcgca gccggatctg ggcgtggcgg
     9301 acctggaagc ccatgccttc cgccgcctgg aggtggcgcg catcgccacc cgggtggcgc
     9361 cgatgctggg tctggtggcg accatgatcc ccctggggcc ggccttgcgg gccctcggcg
     9421 acgggcatat ggcggacgtg tcgcgggggc tcggggtggc cttctcggcg gtgatcctgg
     9481 cgctggtggc ggcgtccctg accttctggg tggtgaacgt gcggcggcgc tggtacgcga
     9541 gcgacctgct ggccattgag gcccgcctct cagggagcgg acgatgaggc tgcgcatgca
     9601 tctgggcgag gcggaggacg acgatccggt gctgtcgctg gtgaacctga tcgacgtgtt
     9661 cctggtgatc gtggcggcct tgctgctggc ggtggcgcag aaccccctgg ggccctttgc
     9721 ggagggcaag gtgaccgtga tccgcggcgc cggcacgccg cagatggagg tggtgatcaa
     9781 ggacggggcg aagatcgagc gcttccgcgg cgatggcggt gccgcctcgg caagcggcgg
     9841 ggtgcgggcg gggacggcct accggctggc ggacgggtcc ctggtctatg tgccagagtg
     9901 agggtcagtc ctccgctgcc cgacggggga ggcggatttc gaacacggcg ccgccgtcgg
     9961 ggtgattgcg ggcttcgatg aggccctggt gtccgcgcac gatctcgcgg gtgatggcca
    10021 ggcccagtcc cgtgccgcct gcgccggtct tggtggcgct gctctggatg aacttgtcga
    10081 agatgcgctc ctcctcgccc ggcggaatcc cgacgccccg gtcccgcacc tggaaggcca
    10141 gggcgtcctg ttcgccgagg agggcgtcgg caaaacgcag ggtcaggacg ccgccatcgg
    10201 gcgagaactt gatggcattg gacagcaggt tctggatggc gtggtagagc tgctgggtat
    10261 ccaccatggc gacggtgctg acggtggcgc gctcgacggc gacgcttagc ccccggctgg
    10321 cgatgagggc ggcgaggtcg ccccggacgc gctcgacgac caccgccacg tcggcggcct
    10381 gcaggcggaa gtccacgcgg cgggcttcca gcttggagag gtcgagcagg tcattcacca
    10441 gcccggcgag gcggctgccg ctctggtgga tgcgattgaa gtacgcggcg atcttgtcgg
    10501 gcggcacctg ccccaatcgc gtcagtccga gccgggcgta ggacaggatg ccgtgcaggg
    10561 gcgtgcggaa ctcatgggac atgttggcga gaaactcgct cttgagctga ttggcgcgct
    10621 cggcggtctc tttggcgctg accaggtcgg cggtctgggc ggcaaccagc tcggatagat
    10681 gatcccggtg ggcccgcagt tccttttcga tggccttcgg gccggtgatg tccttggcgg
    10741 ttccccggta accgaggaag gcgccgtccg gggcgaacag cggcatgccc gagacgctga
    10801 accaggttgt accgatcagc ggatggacga cacggtattc gaagtcgcgg aactgccggc
    10861 gggcgtcgat ctcggcgtgg taggcctgcc acttgacggc gttgcgcaga tcctccggat
    10921 ccgcaaagtc ctcgcgtcgc ttgcccgcga ccacgacgcc cagatcgtgc ccgccccgcg
    10981 gagggccgac gtaggtggtg aagcgcatgt ctgcgtcggt ttgccagaac cagtcggtgg
    11041 acagggccga ataatcgcgg aacagctgtt cgctctcccg cagtgcctgc tcgcgggcgc
    11101 gcagggcggt gatgtcggag ccgacgatga ccacgccatc cagtttgccg ctgcggtcgt
    11161 agagcgtggt ggcgcgcagg atgaagtggc gcacggggtc gcccgccaga cggctgggca
    11221 tgcccgtttc catttcgagg ctgccggcct tcaggatggt ccggaacagc agcgtgccgg
    11281 cacctgcact ggggagatcg gccgccaggg tcgtgagcgt ctccggggcg agcagcgggt
    11341 cgggactctg gccgctcagc ccggccgggt cgatgccgag gcggtggcgc agggcggcgt
    11401 tggcctgacg gatggcgcca aagcggtcga gcaccagcag caggctgtcc atggtctcca
    11461 tcacattggt gacgaaggcg ctcaggcgcg tctgctcggc gctgcgtgcg gccagctcgc
    11521 gggactggaa gctcacgtca tccaccatcg cgtccatcgt cgccgtcacc gcaccgatct
    11581 gctgttccag ggcggcgttg gcctggcgca gggcctccac ttcggcctgg aggcgctgga
    11641 cgtcggccac cggggtgagc tgcagggcgt cggcagcggg caactcgatg atgaactcat
    11701 ccccggcgcg ccggacgggc aggatgttcc ggctgccctg ggtgtccacc tcgagcccgt
    11761 gggccgggca gatcaggcga ccgtcggcat ccagcgcgct ctggtcgagg cggtggcctt
    11821 cgtgcgggca gtgcgccggg acggcgctcc agtgctggcc acggcgcagc acgaggaggt
    11881 gcgtgtcgcg gtcggcgtca ggcatccgca cggtaccgca caggcggtcc gggtggccct
    11941 cgacctggcg caggttgagg atcggcaggt ggcgcggggt gttcacgcga gcaggcgcct
    12001 gatgtgggcc agcgcctcgg gcaagcgggc tgcaacggca gcggagaggc cgggctggaa
    12061 ggggctgagc gtggcgattt cgacgccaat gacgcggacc ctggggggcg tggggagcgc
    12121 caccgggagc aggcgcagca ggctactcac tccggcccca tggatgttgg cggaggccgc
    12181 gtcgtcactg ccctccagtt ctcccgccgc caggctgtgc agggagccgg gcgggccgaa
    12241 gccgcgcagg gcgtccacga cgatggccgt cgtgcagtcc tcgaagcagg ccagcgcgcc
    12301 caggccggcg gtgtctgcgc agaacagccg gaccccgggc ggcaaggggg tctcacccag
    12361 atgcgtgtag acggtcgggc cgaagccgtc gtcgccgtgc agcgcgttgc cgaagcagat
    12421 gatgtgggtc tgttcagacg ccatagcgat gacgggtgcc ccggccgacg acatgcacgg
    12481 tgcacaccag acaggggtca tgggcgcgga tgatgtgtcc gatctcgacc ggatcgccag
    12541 gatcggccag ccgcacgccg atcacgcttt gctcccaatg cccgtggcgc ccgttgctgt
    12601 ccttggggga cgcgttccag gcggtgggtg tgatgatctg gtagcggctg atgcggccct
    12661 gctccacgcg gatccagtga ccgagggtgc cgcgcgcggc ctggacgagg ccgaagcctt
    12721 cgccgtccgc cagggcgttg tccggcggag gcaggaaatg gggcgtgccg agctgaccgg
    12781 ccaggtcgtc cagatggcgc ttcatcaggt gcagggtgag gccggcccgg cgcaggcggg
    12841 cgaactggcg cagccaggtg ttggggcctt cctgccggag caggtcacgg atcaggcggt
    12901 cgccgcccac cagcagttcg gcgagcgggc cggtctcgac gaccgcgtcg ccgtagcggg
    12961 gcgccttggc ccaggtgtag cggtcggagt cgggctggta gtcgggcacg gtctcgccgt
    13021 cgtacggatg gcgtccaccg ggataggggc ggtaccaggc gtggcggacg tgttcgttga
    13081 tcagggccgg atcgaagggc gtcacggtgc catgctgacc gtcatagaga ccggcttcga
    13141 tgagacctgc ggcttcgccg tagggcggct gccagcgttg cggatcgtgc cacgagccgg
    13201 cgctcaggaa atgcccagcg cccgccccga tgtgttgcag gccgatgctg cgggcaaatt
    13261 gggtgagtag gccgagcgcg ctgcctgcgg gggccgcggt gtccagccag gcgaagaagg
    13321 cgtcggccga gtcgagggcg agccagtgct ccagggtgtc gccgatgacg tcctgttcgt
    13381 accattcgcg cgcctgggcc acgatgtcgt ggcaatccac caggtcacgg gcggtggcgc
    13441 cgcgggtgat gccgcccggg agcatatagg aggagtgggg ccactgaccg ccgaagatcg
    13501 ccaccagttc gaggatcttg cgggtggcgc cgagggcgcc ccgcacaatc ctgcccttca
    13561 ggggggcgaa ggcccgttcg acgtcggcga acaggggttc gtcggcgtag cggcggtgga
    13621 ccagatcggg ggtgaagaac aggaagctct ggcgcaggtc gctctgcacg ttttccgcca
    13681 tcaggcagag gttgcggacc agggtggcgt gagggctgac cgggatctgg gcgaggcgct
    13741 ccagggccag ggccgcggtg tacaggtggg cggtgccgca gatgccgcac acgcgcgggg
    13801 tgatcaccag ggcgtcccgc ggcgcacggc cgatcaggag ctgctcgaat ccgcggaaca
    13861 gggtgccgac gcagcgggcg tccacgaccg tgtcgccgtc caggtctacc tggaactcca
    13921 ggtcgccttc gacgcggttc agatcgatgt tgagggttgt caggggcatg gccggggtgg
    13981 cgcctcaggg ttccatttcg cggttcttga cgcggagcgg ggcggctgcc tgggcgaggt
    14041 tcttgtacgc cacgtagtgg gcgcgggaga cgccgagggg cagggtgacc gggacggcgc
    14101 cgatcttctc ggtgcggaac aggtcgccct cccgcgggaa ccccggcgcg gtgcagccaa
    14161 aacacgggac ccccgcgcgt gtcttgctgc tgcgccggtt ccacagttcg ctgttgcagg
    14221 tggcgagggt gctggggccc tggcagccga gattgaagta catgcagccg cgtccgcccg
    14281 gctgggtctc ttcaacgtcg tattcgtggt actcgttgcg tgtgcagccc tgatgcacca
    14341 ttgcgccgaa gaacagacgc ggccggttga ggaaatccag ttcgaggggc tggtcccggg
    14401 ccagcatcgc cagggcgcgg gtgatcgtcg tcggatgggt cgggcagcct gccaggttga
    14461 tgacgggcag gccggcagag gagcgccatt ccggggacag cagaccgccc ggcgattccc
    14521 ggtcgaactg caggccggtg cagtcggtgg ggttgggcgg tgcggcagga atgccgccga
    14581 aggcggcgca ggtgccgatg gcgacgacca cccgggcgcg ggcggcaagc gcttcgacga
    14641 tcctgatctt ggggcgtccg cgatagctgt cgaacatgcc ggagccgtag gggccggtga
    14701 tcaggctccc ttccacgcag agcaggtcca ggggctgggc acctgcctcg accgccgcga
    14761 tgagcgtgcc cagatggccc ggcggccctg ccgtgagcga cgggtgccag aggatgtcga
    14821 gcgtgccgtc ggcggtgagg cgttcgatgg aggggctgtc ggcgttcagc agggacaggg
    14881 tgtcaccgct gcaggcaccg gtctgcagcc agatcagggt ggccatgggg acgatctcca
    14941 gagtccgtca tgcctcctct cggaaggcga acggggcgcg caccggtacg cgacaggatg
    15001 taccggaagc ttgccgcctc tgggtctacg tcgtgtgccg tgagaacttg agtccgctgc
    15061 tcaggattca gcggccggca gtggaccgtt gttcggcctc aagggcccgg cgcagggcca
    15121 gatcggtgcc gccaaggggc cacaggtgcg ccgacagata cagcccggtg cagctgagcg
    15181 tggtgctgcc gggcttctgc tcgaccagat ccacgtcgag gcacgcctgg gccaggggcg
    15241 accggtccgc gtcggtcacc agcagggtgc cgcaggcgca acaggcccag gggctgtagc
    15301 cctcaggcgg gggctcacgc agggcgggcg cccagcgcag gcgttcccgt tcgtccggtg
    15361 cctggccgtc gtagagcagc cacaagggac cgaagctttc acgcagggag cccaggaaca
    15421 gcccggcttc cgggctgatg cggagcctca gggcgtcgag caggccgtcg tcgggggaca
    15481 ttgcggtggc cgggccaagg cttcacgcgc cgcgcacggg cggcagaacg gtggccagtt
    15541 cggcgtcggt aaaggggcgc gaggtggtct cgaaggcgcg tccgcggccg cagtcgaggg
    15601 agaagtcgcc gccctgaccg ggtcgcacgt cgagggtgag ctggctgttc tcgtaataga
    15661 tgtattcctg gtggccgagc cagaactcgg cgccgccgac cgtgcccagc agcagggtgg
    15721 tctggctggg catgaattcg cccaccttga agcagttggg gacgctgcca tcgcagcagc
    15781 cgccggacag caggaagatc accgggccga agtcgcggcg cagttcgtcg atgaactgga
    15841 cggcggcctc ggtggcgacg atgcgttcgg tcatggacgg atctccggaa tcgaacaaaa
    15901 gcactggacg aaaaaaacgg ggcgagagac tcgccccgta acaccttgcc ttacatgaaa
    15961 cgctcagatc agaagaagcc cagcttctgc tcggcgtagc tgaccagcag gttcttggtc
    16021 tgctggtagt ggtcgagcat catgtggtgg gtttcgcgac cgatgccgga ctccttgtag
    16081 ccaccgaagg cggcgtgggc ggggtaggcg tggtagcagt tggtccacac gcggccggcc
    16141 ttgatggcgc gacccatgcg gtaggcgacg ttgccgttgc ggctccacac gccggagccg
    16201 aggccataga cggtgtcgtt ggcgatcgcg acggcttcgg cttcgtcctt gaaggtggtc
    16261 acggccagca ccggcccgaa gatctcttcc tggaagatgc gcatcttgtt gtggcccttg
    16321 aacagggtcg gctggatgta gtagccctcg gagaggtcgc cgctcaggtg ggcgcgctca
    16381 ccaccgatca ggcactgggc gccttcctgc ttgcccacgg ccaggtattc ggtgatcttg
    16441 tcgatctgca tctgggacgc ctgggcaccg atcatggtgt cggtgtcgag ggggctgccc
    16501 tgcttgatgg cggccacacg gggcagcacg cgctcgatga acttgtcgta gatggattcc
    16561 tggatcaggg cgcgggacgg gcaggtgcac acttcgccct ggttgaaggc gaacagcacc
    16621 atgccttcga tggccttgtc gaggaagccg tcgtccttgt ccatcacgtc ggcgaagaag
    16681 atgttggggg acttgccgcc cagttccagc gtggcgggga tcaggttgga ggcggcggcc
    16741 tggccgatga tcttgccggt ggcggtggag ccggtgaagg cgatcttggc gatgcgcttg
    16801 ctggtggcc
//
LOCUS       DAOQPA010000081        16691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1732_length_16691_cov_21.079286, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000081 DAOQPA010000000
VERSION     DAOQPA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16691
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_16691_cov_21.079286"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2285)
                     /gene="pilQ"
                     /locus_tag="PKX01_11615"
     CDS             complement(96..2285)
                     /gene="pilQ"
                     /locus_tag="PKX01_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HMZ76820.1"
                     /translation="MRTGNVRVGLGVMFASFWLGCSVPVGLAFAQEPAASTAEASNRI
                     ERILATEQGGNIIVKLSMAQPLSRVPASFSVASPARIAFDFPDTANGLGRNSQTFNTG
                     DLRSANIVQVGDRTRLVLNLARVSPYDARVEGNDVLITLAAAQLKEAAQALPATQFAP
                     VPLNSGDRHAIREINFRRGKDGEGRLVVDLSDPNTGIDIRQQGNALIVDFLKTDVAEQ
                     LRRRLDVVDFGTPVNSVVTQAQGGNVRMTVTPKGLWEHNAYQSDSQFVLEVKPVREDP
                     NKLVKGAGKGQFGGEKLSLNFQNIDVRSVLQVIADFTNFNIITSDSVQGNVTLRLKDV
                     PWDQALDIILQARGLDMRKNGNVIWIAPRDELAAREKMDLESKAQIGDLEPLRTESFQ
                     INYHKAKAVSEFLKSKDQTVLSKRGSVIVDERSNKLFVNDVASRLEDLRRLIAEIDVQ
                     VRQVMIEAQIVEATDNFARNLGMRLGIGGKTGAYLGQTQSSSTSTNSVSVYRNTIGGG
                     LGSTGYQAGQIETKPDFFKDALNLNLPASSINGRQAGAISFALWNSSATRYLDLEVSA
                     LEADGRGKVVSRPRVMTADQAEAVIEQGVEIPYQSATSSGATSVTFRKANLALKVKPQ
                     VTPDGKIMMSLDINKDSPRFDVSLTSGVPIDTKHVKTEVIVENGGTIVIGGIYQQDVS
                     NTTTKVPFLGDLPGLGYFFRNNERKDNKSELLVFITPRILNEQLGLR"
     gene            complement(2282..2815)
                     /locus_tag="PKX01_11620"
     CDS             complement(2282..2815)
                     /locus_tag="PKX01_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HMZ76821.1"
                     /translation="MRYERLTLLPLLAAALILAGCGDHEDDLRSWMADVSKDLKPNLK
                     PLPPIQQAETVTYQGAGQVDPFRPTKIEPDKKSGMFVPDANRRREPLEAYPLETLKMV
                     GVLIKGGQSHAIIAADKTLHQVHVGNYMGQNYGRVTAISESEVTLKELVEDANGEWVE
                     RVSTLQLQEQAPQEAKK"
     gene            complement(2812..3486)
                     /locus_tag="PKX01_11625"
     CDS             complement(2812..3486)
                     /locus_tag="PKX01_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HMZ76822.1"
                     /translation="MALKDRIERLRAIDFQALVEDFKGLDPNNPGQWPLAPRGAALVA
                     VFAGIVALGWWFDWSEQMAELDQKTQEESRLKDEWLGKKRQAVNLAEYKRQLGEIDRQ
                     FGALLKQLPNRSEIESLLSDINQAGLGRGLQFELFKPGAEAAKDFYAELPIAIRVTGS
                     YHDLGDFVSDVAQMSRIVTLNDMAIEAGKDGALKFDATAVTYRYLDEEEVAKARKDKS
                     KDKAGK"
     gene            complement(3486..4088)
                     /locus_tag="PKX01_11630"
     CDS             complement(3486..4088)
                     /locus_tag="PKX01_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HMZ76823.1"
                     /translation="MIRINLLPHREEKRKARRQQFFLLSALMVVAGLFVWFVGHTILG
                     GYLSGQDAKNAFLKEEIAALDKDIVEIKRLREQSEALLSRKQVIESLQTHRTETVAVF
                     NELARLMPEGVYLKALKETGATINLQGFAQSNARVSMLMRNLDGSQYLERPELVEVKA
                     AMINNRRVSEFNMNVSVKRNKADGPGAKGKSDVPQAGGKS"
     gene            complement(4085..5161)
                     /locus_tag="PKX01_11635"
     CDS             complement(4085..5161)
                     /locus_tag="PKX01_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HMZ76824.1"
                     /translation="MIDLSFLNPKARPLIGVDISSSSVKMVELSGVAGGGYRVERYAI
                     ESLPRDAVVDGNIANLEAVSEAIRRGWRRLGTATKFVAMALPASAVITKKIILPAGLR
                     DQELEVQVESEANQYIPFALDEVNLDFQVLGPAGSGADEDEVLIAASRKEKVEDRVAA
                     AEAAGLKALVMDVESYAALAAFELVQVQLPDEGEDKIIAVVDAGANVMNVSVLRNGNQ
                     VYAREQAFGGYLLTQDIMRHFGMSMEEAENAKRTGALPENYESDLLRPFMDSLALEVT
                     RALQFFFTSTQFNQVDHIVLAGGCAVIPGLAETVAGRSQVDTIVANPFANMTLSSKVR
                     PKNLAADAPALLVACGLALRRFDS"
     gene            5335..7620
                     /locus_tag="PKX01_11640"
     CDS             5335..7620
                     /locus_tag="PKX01_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HMZ76825.1"
                     /translation="MRWVLYPLTFLLGLVVLGIAVLALAVAFAWPTLPSLEALTDYHP
                     KIPLRVYTADGALIGEYGDERRAFIHIQDVPPVLKNAILAAEDDRFYQHSGVDLLGIL
                     RAAGTNIVTGSKRQGSSTITMQVARNFFLTREKTYSRKLYEILLAFKIEQNLTKDQIL
                     EVYINQIFLGRRAYGFAVAAHTYFGKSIRDLNIPEAAMLAGLPKAPSAYNPIVNPKRA
                     ALRQQYVLRRMTELGFISDAEQQQALATPLKYASSSAEFGGSADYVAEMARQIAVEQF
                     KDEAYTRGIKVITTIDRGEQEAAYRALRRGVMDYDRRHGYRGAEAYVDPTRLGGEQQG
                     ENLDEVLADTPDYDDLKAAVVTQAAPGAVTVYRYGETIRIAGEGLRFAAAMLGDKAPP
                     NKRIRPGAIVRIAQTDNKAWEITQLPEVEGALTAADPRTGAIRALVGGFDFNRNKFNH
                     VTQAWRQPGSSFKPFIYSASFEKGYGPNSIVDDSPLSFPAGETGSQAWEPKNYDGKYD
                     GPISLRTALARSKNMVSIRLLKSIGPRYAQDYVTKFGFDAERHPPYLTMALGAGSVTP
                     WQMLRAYAVFANGGYRVEPYLVKEIRDESGAILARVDPPVAGETAERAIDARNAYLMD
                     SMMKDVVRRGTATRALTLKRGDLAGKTGTTNDYIDAWFCGYQPTVVAVSWIGFDQPRK
                     LGSGETGGAAALPMWIGYMRRALEGVPESYPPAPDGLIRVQPGGGGTEELIYKENLPA
                     VPTEAPVEAPHEGPADEAPPR"
     gene            complement(7641..7961)
                     /gene="cyaY"
                     /locus_tag="PKX01_11645"
     CDS             complement(7641..7961)
                     /gene="cyaY"
                     /locus_tag="PKX01_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HMZ76826.1"
                     /translation="MDEARFNALADAELARLEALLDACDVEFDYEVKPGGVLELEFDN
                     GTKIIVNRHTAAREIWLAAKSGGFHFRPQEGRWVGTRDGEDFYAVLSRCMGEQAGVQV
                     RIAG"
     gene            8017..8181
                     /locus_tag="PKX01_11650"
     CDS             8017..8181
                     /locus_tag="PKX01_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HMZ76827.1"
                     /translation="MRTLSIAALVCLLLSACGIKGPLYLPQVPPPKSKPATQAPAADH
                     NKPAPQDSAP"
     gene            8178..9440
                     /gene="lysA"
                     /locus_tag="PKX01_11655"
     CDS             8178..9440
                     /gene="lysA"
                     /locus_tag="PKX01_11655"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMZ76828.1"
                     /translation="MTTALPMPTLSAGTAGLNLEGVALASIAERFGTPTYVYSRAALT
                     SAYAAYQQALGERKALVCYAVKANSSLGVLSVFARLGAGFDIVSGGELARVLAAGGDA
                     GKVIFSGVGKTAAEMRFALDAGIGCFNVESAAELDRLNDVAAAAGKRAPIAFRVNPDV
                     NPNTHPYISTGLRSNKFGVAFDEALDLYRKAARLPHIEISGIDCHIGSQLLDPAPMTE
                     AAEKILGLVDQLGREGIKLDHIDLGGGLGIRYRDEEPPTPSIYLKPLLDCFAGRDEAL
                     CFEPGRSLVGNAGLLLTRIEYLKPGVEKNFAIIDAAMNDLARPALYDAYHEIVAVAPR
                     AVPASRYEVVGPICESGDFLGHDRDLSVAAGDLLAILSAGAYGMTMSSNYNTRPRAAE
                     VLVDGDAVHLIRERETLSHLLKGETPLA"
     gene            complement(9518..10921)
                     /locus_tag="PKX01_11660"
     CDS             complement(9518..10921)
                     /locus_tag="PKX01_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMZ76829.1"
                     /translation="MLAPRLAFIDVETTGANPVVDRVTEIAILRVEDGRLVERWSSLV
                     NPGIPIPPTIQRFTGITDAMVAQAPGFDALKDGVRALFADCVFVAHNARFDYGFIKNE
                     FRRIGEAFDAPVLCTVKLSRALFPQYHRHGLDALIERHGLECTARHRAMGDTDALWQF
                     AQLVCGQFEAPVLETAVLKAMKAPSRPAGLPEGALEGLPEGPGVYLFYGENDLPLYIG
                     KSVSLRSRVMSHFSADHRNAKEARIAQQVRRVDWQTTAGELGALLLESRLVKARKPTE
                     NRQLRETEAVFGLRYDGARKRGPVLLREALNGSDPAAWEAVYGAFRSKREADNALRDL
                     AALYKLCPRRIGTEPWGEGACMAHQMKRCAGVCAGRESPDEHDARLLKVLESLRLRRW
                     PWDGPVAIREHHGETGRSAVHVVDRWCLLGSVEDEGALGELLAAPSPRSFDLDVYRVL
                     SRWLDSPAARGAVTPLG"
     gene            complement(10962..11150)
                     /locus_tag="PKX01_11665"
     CDS             complement(10962..11150)
                     /locus_tag="PKX01_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HMZ76830.1"
                     /translation="MAIYESEHTQFMREWLKNHPEEVSVQQAGRALWWDRPQNPELNE
                     GFAVGKIEQKPYPYSTEG"
     gene            complement(11202..13421)
                     /locus_tag="PKX01_11670"
     CDS             complement(11202..13421)
                     /locus_tag="PKX01_11670"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HMZ76831.1"
                     /translation="MESAASAPLGRSGVFQPPASSVLPLDFGRLRAQVATYLRPEDVG
                     RIEAAYHFAADAHSGQFRISGDPYISHPVAVAEILAGWHLDAQALCAALLHDVMEDTH
                     ITKSEIAERFGRTTAELVDGVSKLDKIEFQSQEEAQAENFRKMLLAMASDLRVILIKL
                     ADRLHNMRTLDCVRPDKRRRIARETQEIHAPIANRLGLNALYRELQELAFKHVHPMRF
                     RVLSKAILAARGNRREVVGKILAAIEERLPQWNIQAEVRGREKHLYSIYRKMNEKRLS
                     FSQVLDIYGFRVIVPDVQTCYRALGALHGLYKPVPGKFKDYIAIPKANGYQSLHTTLI
                     GPFGTPLEVQIRTAQMNHVADSGVASHWLYKEDEKTLTDLQAKTHTWLQSLLELQSTS
                     GDSSEFLEHVKVDLFPGEVYAFTPKGKIFALPRGATVVDFAYAVHTDVGNRCVAGRVN
                     GELLPLRTELRNGDQVEIITAAHASPNPAWLSYVRTGKARAQIRHFLKNAHQEEAFFT
                     LGERLLNQALRPHGLTLGSVSAIAWERYLREFTLANRKELMTDIGLGRRLSAIVARRL
                     AEIQDIESGRADVVKPKPAGAILIRGTEGVAVQLARCCRPIPGDPIVGIIRKGQGLEV
                     HMHDCPQARKLRGERERWIDVEWEPGVDRLFDVTLRLLTHNVRGVLAKVANAIAEQDC
                     NIQNVSTDGEQGAFSTIYLTVQVTHRLHLAKVIRGVRNIPEVVRIGRLKGDPKLPVA"
     gene            complement(13444..13659)
                     /gene="rpoZ"
                     /locus_tag="PKX01_11675"
     CDS             complement(13444..13659)
                     /gene="rpoZ"
                     /locus_tag="PKX01_11675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMZ76832.1"
                     /translation="MARITVDDCLKRISNRFQLTLAATYRARQLTAGATPQVELDRHD
                     KDKPTVIALREIAAGKVGTEVLNRGQA"
     gene            complement(13663..14277)
                     /gene="gmk"
                     /locus_tag="PKX01_11680"
     CDS             complement(13663..14277)
                     /gene="gmk"
                     /locus_tag="PKX01_11680"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMZ76833.1"
                     /translation="MPGTLFIVTAPSGAGKTTLVSGLLKRDAQVSLSISYTTRTPRAG
                     ETHGVHYHFVDVPTFRALRDKGEFLEWAEVHNNYYGTSRRWLEEQMASGRDILLEIDW
                     QGAQQVRKAFPKAVGVFILPPSVDELERRLRGRGTDSEEVIARRVLGARGEMRHVGEF
                     DYVIINQDLSAALEDLVAVVRAARLRYANQAARQPEFFHYLEQD"
     gene            complement(14283..15149)
                     /locus_tag="PKX01_11685"
     CDS             complement(14283..15149)
                     /locus_tag="PKX01_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMZ76834.1"
                     /translation="MIHSMTGYAVQTRELGRVALHLELRSVNSRFLDLAFRIADELRQ
                     SEPQLREALTGRLSRGKVECRFHLQGKESATAELVLNGGLLGQLKAVEAAVRAELPAA
                     APLSVGEILRWPGMLGDDGVGFEQIQPQIAPLLAAALEDLVASRRREGEKLADMIRGR
                     IAHMRDLVARVQPRVPALVAEYQEKLAQRLRDASATLDDDRIRLEVGLFAQRVDVAEE
                     LSRLSTHLDEVERILKSGGTAGKRLDFLMQELNREANTLGSKAMAADMTAIAVELKLA
                     IEQMREQVQNIE"
     gene            15236..16186
                     /locus_tag="PKX01_11690"
     CDS             15236..16186
                     /locus_tag="PKX01_11690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ76835.1"
                     /translation="MATQQNCPLPPGFQLDHYRVEKQLSLGGFSIVYLAYDQDGTPVA
                     IKEYLPNSLALRAQGEITPHVTEAHQSAFRYGMKCFFEEGRALAKLVHPNVVRVLNFF
                     RANGTVYMVMQFERGRTLHDYVQKHKGNIRERFIRGVFTRMLNGLREVHSHKLLHLDI
                     KPSNIYLRADGNPVLLDFGAARQTLASDTPILKPMYTPGFASPEQFTNREALGPWSDI
                     YSVGASMYACLAGTSPQRSDERLNNDTVVPASKLWVGQYSPHFLSTIDWCLQLDPLKR
                     PQSCFALQKVLAQRHREAPPEPKTWLTGLGDRIKTIMGRT"
     gene            16183..>16691
                     /locus_tag="PKX01_11695"
     CDS             16183..>16691
                     /locus_tag="PKX01_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ76836.1"
                     /translation="MRFTIYQESRVGKRRTNQDRVAYSYTRDALLLVLADGMGGHLYG
                     EVAAQISVQFIAQSFQQEARPKVSDPILFLSRVLSNAHHAILDYAFDKQLSDVPRTTI
                     VACLIQDGVAHWAHAGDSRLYLIRRNKVFAQTRDHSRVQLMLDQGLLDAEAAASHPGR
                     NRIFSCLGG"
BASE COUNT         3000 a         5694 c         5036 g         2961 t
ORIGIN      
        1 aggctcatca ccacccccag aacccccagc agggcccagg acaaaaacgt gcgcagaatc
       61 gattgatgca tgaggtgttc catccagagt ccggattaac gcaggcccag ctgctcgttg
      121 aggatacggg gcgtgatgaa gaccagaagt tcggacttgt tgtccttgcg ttcgttgttt
      181 ctgaagaaat aaccgagacc gggcagatcg ccgaggaacg ggaccttcgt cgtcgtgttg
      241 gagacgtcct gctgataaat gccgccaatg acgatggtac cgccgttctc gacgatgacc
      301 tcggtcttca cgtgcttggt gtcgatcggc accccggacg tgagggagac gtcaaaccgg
      361 ggagaatcct tgttgatgtc gagggacatc atgatcttgc cgtcgggggt gacctgcggc
      421 ttgaccttga gggccaggtt tgccttgcgg aaggtcacgg acgtggcacc cgagctggtc
      481 gccgactggt aggggatctc gacgccctgc tcgatcaccg cctcagcctg atcggccgtc
      541 atgacccgcg gacgggaaac gaccttgccg cgaccatcgg cttcgagggc cgacacttcc
      601 aggtccaggt aacgggtcgc cgagctgttc cacaacgcga aggagatggc gcccgcctga
      661 cgaccgttga tgctggaagc cggaaggttg aggttgagcg cgtccttgaa gaaatccggc
      721 ttggtctcga tctgcccggc ctggtaaccg gtcgacccca gcccaccgcc gatggtgttg
      781 cgatagacgg agaccgaatt ggtggaggtg ctcgaggact gggtctgacc gaggtaggca
      841 ccggtcttgc cgccgatgcc cagacgcatg cccaggttgc gtgcgaagtt gtccgtcgct
      901 tcgacgatct gcgcctcgat catgacctgg cgcacctgca cgtcaatctc ggcgatcagg
      961 cgacggagat cctcgagccg ggacgccacg tcattgacga agagcttgtt gctgcgctcg
     1021 tcaacaatca cgctgccacg cttggacagc accgtctggt ccttgctctt gaggaactcc
     1081 gaaaccgcct tggccttgtg gtagttgatc tggaagcttt ccgtgcgcag gggctccaga
     1141 tcgccgatct gggccttgga ctcaagatcc atcttttccc gggcggccag ttcgtccctc
     1201 ggcgcgatcc agatcacgtt gccattcttg cgcatgtcga ggccgcgagc ctgcaggatg
     1261 atgtcaagtg cctggtccca gggcacgtcc ttgaggcgga gggtgacgtt accctgaacg
     1321 gaatcgctgg tgatgatatt gaagttggtg aagtcggcga tcacctgcag caccgaacga
     1381 acatcgatat tctggaagtt gagggagagc ttttctccac cgaactgccc cttgccggcc
     1441 cccttgacga gcttgttggg atcctcgcgg acgggcttga cctccaagac gaactggctg
     1501 tcactctggt aggcgttatg ctcccagagg cccttgggcg taacggtcat gcgcacattg
     1561 ccgccttgcg cctgggtcac caccgagttg acgggtgtcc cgaaatccac cacatcgagc
     1621 ctgcggcgca attgctcggc cacatcggtc ttaaggaaat cgacgatgag ggcgttgccc
     1681 tgctggcgga tatcgatgcc cgtgttggga tccgaaagat ccacgaccag acgcccttct
     1741 ccgtccttgc cacgccggaa attgatttca cggatggcgt ggcgatcgcc ggaattgagt
     1801 ggcaccggcg cgaactgggt cgccggcaat gcctgcgcag cctccttgag ctgggcggcc
     1861 gccagggtga tcagcacatc attcccctcg acccgcgcat catagggtga cacccgcgcg
     1921 aggttcagga ccaggcgcgt gcggtcaccg acctgtacga tattggcgct gcgcaggtcg
     1981 ccggtattga aggtctgact gttacgaccg agcccattgg ccgtatcggg gaaatcgaag
     2041 gcaatccgtg caggagacgc cacactgaag ctggccggca cacgtgacaa gggctgcgcc
     2101 atggacagct tgacgatgat gttgccccct tgctcggtgg caaggatccg ctcgatacgg
     2161 ttgctcgctt cagcagtcga ggcagccggc tcctgggcaa acgccagtcc gaccggcacg
     2221 ctgcatccca gccagaacga cgcaaacatc acgcccagac cgaccctgac atttcctgtt
     2281 ctcatttctt cgcctcctgg ggcgcctgct cctgcaactg cagcgtactg acgcgctcga
     2341 cccattcgcc gttcgcatcc tcgacgagtt ccttgagggt gacttcggac tcactgattg
     2401 ccgtcacccg accgtagttc tgtcccatgt aattgccgac gtgcacctga tgcagcgtct
     2461 tgtcggccgc gatgatcgca tgagactgcc cgcccttgat caggaccccc accatcttga
     2521 gggtttcgag cgggtaagct tcgagaggct cacgcctccg gttcgcatcc ggcacgaaca
     2581 tgccggattt cttgtcgggt tcaatcttgg tcgggcggaa cggatccacc tgccctgccc
     2641 cctgataggt caccgtctca gcctgctgga tgggtggcaa gggcttgaga ttgggcttga
     2701 ggtccttgga gacatccgcc atccagctcc tcaggtcgtc ctcatgatca ccgcagcccg
     2761 cgaggatcaa cgcggccgcc agcagcggca gaagggtcag tcgctcgtat ctcacttgcc
     2821 cgccttatcc ttggacttgt ccttgcgcgc cttggccact tcctcttcat ccaggtagcg
     2881 gtaggtcacg gccgtcgcgt cgaacttgag tgctccatcc ttgccggcct cgatggccat
     2941 atcattgagc gtgacgatgc gcgacatctg ggccacatca ctgacgaaat cgcccaggtc
     3001 gtgataggac ccggtcaccc ggatggcgat cggcagttcg gcgtagaaat ccttggcggc
     3061 ttcggccccg ggtttgaaga gttcaaactg cagaccacgc ccgagtccag cctgattgat
     3121 gtccgacagc aggctttcga tctcggaccg attggggagc tgcttcagca gcgcaccgaa
     3181 ctgccggtcg atctcgccaa gctggcgctt gtactctgcc aggttcaccg cctgccgctt
     3241 cttgcccagc cattcatcct tgagacggct ttcctcctgg gtcttctgat ccagttcggc
     3301 catctgctcg ctccagtcga accaccagcc cagtgccaca atcccggcaa agaccgccac
     3361 caaggcagca ccgcgcggcg cgaggggcca ttgtccgggg ttgttcggat caagcccctt
     3421 gaaatcctcg acgagggcct ggaaatcgat ggcacgcaag cgttcgatac gatccttgag
     3481 tgccatcacg acttccctcc cgcctgcgga acgtccgact tgcccttggc gccggggccg
     3541 tcggctttgt tgcgcttgac ggaaacattc atgttgaact cactgacccg acggttattg
     3601 atcatcgccg ccttcacttc aaccagttcg ggacgttcga gatattgcga cccgtccaga
     3661 ttgcgcatca gcatcgagac ccgcgcgttc gactgcgcaa atccctgcag attgatcgtc
     3721 gcgccggttt ccttgagtgc cttgagataa accccctctg gcattagtcg ggccaattcg
     3781 ttaaagaccg cgacggtctc ggtacggtgc gtctgcaagg attcgatcac ctgcttgcgc
     3841 gacagcagcg cctcggactg ctcccgcagt cgcttgatct cgacgatatc cttgtcgagg
     3901 gccgcgattt cctccttgag gaaggcattc ttggcatcct gtccgctgag gtagccgccc
     3961 aggatcgtat gtccgacgaa ccacacgaac agcccggcca ccaccatcag ggccgacagc
     4021 aggaagaact gctgccgtct cgccttgcgt ttttcctccc ggtgcgggag caggttgatg
     4081 cggatcacga atcaaacctc cgcagggcca gtccgcacgc caccagcaag gccggcgcat
     4141 cggctgcgag gttcttgggg cggaccttcg aggaaagggt catgttggcg aacgggttgg
     4201 caacgatggt gtccacctgg gatcgcccgg ccaccgtctc cgccagcccg ggaatgaccg
     4261 cacaccctcc ggcgagcacg atgtgatcga cctggttgaa ctgggtcgag gtgaagaaga
     4321 actgcaaggc gcgcgtcact tccagcgcca ggctgtccat gaaggggcgc agcagatcgg
     4381 attcatagtt ctcgggcagg gcgccggtac gcttggcgtt ttcagcctct tccatggaca
     4441 tgccgaagtg acgcatgatg tcctgggtga ggaggtagcc accgaaggcc tgctcccgcg
     4501 catacacctg attgccgttg cgcagcaccg agacgttcat gacattggcg cccgcgtcca
     4561 cgacggcgat gatcttgtcc tccccttcgt cgggcaactg cacctggacc agctcgaatg
     4621 ctgccagcgc cgcataggac tccacatcca tcaccagcgc cttgagaccc gccgcctccg
     4681 ccgccgccac acggtcctcc accttttcct tgcgagacgc tgcaatcagc acttcgtcct
     4741 catccgcacc ggagcccgcc gggccgagca cctgaaaatc cagattgacc tcgtccagcg
     4801 caaagggaat gtactgattg gcctcggact cgacctggac ttcgagctcc tggtcacgca
     4861 accctgccgg caggatgatc ttcttggtga tgaccgccga cgccggcaat gccatggcga
     4921 cgaatttcgt ggccgtcccc aggcgccgcc agccgcgccg gatggcttcg gacaccgctt
     4981 ccaggttggc gatgttgccg tcgacgaccg catcacgcgg cagggattcg atcgcgtagc
     5041 gttcgacccg gtaccctccg cccgccacgc cggacagttc gaccatcttg accgacgacg
     5101 acgaaatgtc gacgccgatc aaaggccgcg ccttcggatt gagaaaggat aggtcgatca
     5161 caaaaagtcc tgaaaattcg taacgttagg cgcaataaca acaatctact tgaaatccta
     5221 acaacaacca tgccgggtgt aaagcaagcc cgggtaaaac cacgccacac gcaacggccg
     5281 acgaggcggg cctccccgta taatggcgaa ccgtttccgt ctgccggaca ctccatgcgc
     5341 tgggtactgt accccctgac cttcctgctc ggcctcgtcg tgctgggcat cgctgtcctt
     5401 gccctggcgg ttgcctttgc ctggccgacc ctgccgtccc tggaagcgct gacggactat
     5461 caccccaaga tcccactacg cgtctacaca gccgatggcg cgctgatcgg cgaatacggg
     5521 gacgaacgtc gcgccttcat ccatatacag gacgtcccgc ctgtcctcaa gaatgccatc
     5581 ctcgccgccg aggacgaccg cttctatcaa cattccggcg tggacctgct cggcatcctg
     5641 cgggctgccg gcaccaacat cgtcaccggc agcaaacgcc agggctcctc caccatcacc
     5701 atgcaggtgg cccgcaactt cttcctcacc cgggagaaaa cctactcccg gaaactttac
     5761 gaaatcctgc tcgccttcaa gatcgagcag aaccttacca aggaccagat cctcgaggtc
     5821 tacatcaatc agatcttcct gggccgccgc gcctacggct ttgccgtcgc cgcccacacc
     5881 tatttcggca aatccatccg ggatctgaac attcccgaag ccgccatgct ggccggcctg
     5941 cccaaggcgc cttccgccta caaccccatc gtcaacccca agcgcgcggc cctgcgccag
     6001 caatatgtcc tgcgccgcat gactgaactc ggcttcatct ccgatgcgga gcagcagcag
     6061 gccctcgcca cgccgctcaa atatgcttcc agcagcgccg aattcggcgg cagcgccgat
     6121 tacgtggccg agatggcgcg ccagatcgcc gtggaacagt tcaaggacga ggcctacacc
     6181 cgcggcatca aggtcatcac caccatcgat cggggcgagc aggaagccgc ctatcgggcc
     6241 ctgcgccgcg gcgtgatgga ctacgaccgc cgccacggct accgcggcgc cgaggcctac
     6301 gtcgacccca cccgcctggg cggcgagcag cagggcgaaa acctcgacga agtcctcgcc
     6361 gacacccccg actacgacga cctgaaggcc gccgtcgtca cccaggccgc accgggcgcc
     6421 gtcacggtct accgctacgg tgaaacgatc cgcatcgcgg gcgaaggtct gcgcttcgct
     6481 gcggcgatgc tcggcgacaa ggccccgccc aacaagcgca tccgccccgg cgccatcgtg
     6541 cgcattgccc agacggacaa caaggcctgg gagatcaccc aactgcccga ggtcgaaggc
     6601 gcgctcaccg ccgccgaccc gcgcactggc gccatccgcg ccctggtggg cggcttcgac
     6661 ttcaaccgca acaagttcaa ccacgtcacc caggcctggc gacagcccgg ctctagcttc
     6721 aagcccttca tctactccgc ctccttcgaa aagggttacg gccccaacag catcgtggat
     6781 gattcgcccc tgtcctttcc ggcgggcgag accggcagcc aggcctggga acccaagaac
     6841 tacgacggca agtacgacgg ccccatttcc ctgcgtacgg ccctggctcg ctccaagaac
     6901 atggtgtcga tccgcctgct caaatccatc ggtccgcgct acgcccagga ctacgtgacc
     6961 aagttcggct tcgacgccga acgccacccg ccctacctca ccatggccct gggcgccggc
     7021 tccgttacgc cgtggcagat gctgcgcgcc tatgccgtct tcgccaacgg cggctatcgc
     7081 gtcgaaccct atctggtaaa agagatccgc gatgaatccg gcgcgatcct cgcccgcgtc
     7141 gatccgcctg tggcgggcga aacggccgag cgggccatcg acgcccgcaa cgcctatctg
     7201 atggactcca tgatgaagga cgtggtccgg cgcggcaccg ccacccgcgc gctgaccctc
     7261 aagcgcggcg acctggccgg caagaccggc acgaccaacg actacatcga cgcctggttc
     7321 tgcggctatc aacctaccgt ggtggccgtg tcatggatcg gtttcgacca gccgcgcaag
     7381 cttggctccg gcgaaaccgg cggcgccgcc gcgctgccca tgtggatcgg ctacatgcgc
     7441 cgcgcgctcg aaggcgtgcc cgaaagctac ccgcccgcac ccgacggcct gatccgcgta
     7501 cagcccggtg gcggcggaac cgaagaactg atctacaagg aaaacctgcc ggcggtcccg
     7561 actgaagccc ccgtcgaggc gccccatgag ggcccggccg acgaagcgcc gccccgctga
     7621 gcgggtgcgg cacaccggct tcagcctgcg atgcgcacct gcacgccagc ctgttcgccc
     7681 atgcagcggg acagcacggc atagaaatcc tcgccgtccc gcgtgcccac ccagcggccc
     7741 tcctggggcc ggaaatggaa gccacccgac ttggccgcca gccagatctc ccgcgccgcc
     7801 gtatgccggt tcacgatgat cttcgtgcca ttgtcgaact ccagttccaa cacaccgccc
     7861 ggcttcactt catagtcgaa ctccacatcg caggcatcga gcaaggcttc cagacgcgcc
     7921 agttccgcgt ctgccagtgc gttgaatcgt gcttcatcca tggccatctt cccgatctgt
     7981 taacattgtc gtttttgcgc gcgacccatg cgacccatgc gcacactttc catcgctgcc
     8041 ctcgtctgcc tgctcctgag cgcctgtggc atcaagggcc cgctctatct gccccaggtg
     8101 ccgccgccca agtcgaagcc ggccacacag gcacccgccg ccgatcataa caaacccgct
     8161 ccccaggatt ccgccccatg accaccgctt tgcccatgcc gacgctcagc gccggcaccg
     8221 ccggcctgaa cctcgaaggc gtggcccttg ccagcatcgc cgaacgtttc ggaacgccca
     8281 cctacgtcta ttcccgtgcc gccctcacct ccgcctacgc ggcctaccag caggccctcg
     8341 gcgaacgcaa ggcgctggtg tgttacgccg tcaaggccaa ctccagcctg ggcgtcttgt
     8401 cggtgttcgc caggctgggc gccggcttcg acatcgtctc cggcggggaa ctggcccgcg
     8461 tactggctgc gggtggcgat gccggcaagg tgatcttctc cggtgtcggc aagaccgctg
     8521 ccgagatgcg ctttgcgctg gacgccggca tcggctgctt caacgtggaa tccgccgccg
     8581 aactcgaccg cctgaatgac gtcgccgccg cggcgggcaa gcgcgcgccg attgccttcc
     8641 gcgtcaatcc ggacgtcaat cccaacaccc acccctacat ctccacgggg ctgcgcagca
     8701 acaagttcgg cgtggccttc gacgaggccc ttgatctgta tcgcaaggcg gcgcgcctgc
     8761 cccacatcga gatctccggc atcgactgcc acatcggctc ccagctcctc gacccggccc
     8821 ccatgaccga agccgccgag aagatcctcg gcctggtgga tcagctcggc cgcgaaggca
     8881 tcaagctcga ccacatcgat ctgggcggcg gcctcggcat ccgctaccgt gacgaggaac
     8941 cgcccacccc gagcatctac ctcaagcccc tgctggactg cttcgccggc cgcgacgagg
     9001 ccctgtgctt cgagcccggt cgctccctgg tgggcaacgc gggcctgctc ctgacccgca
     9061 tcgaatacct caagcccggc gtcgagaaga acttcgccat catcgacgcg gccatgaacg
     9121 atctggcccg cccggcgctc tacgacgcct atcacgagat cgtcgccgtg gcaccccgcg
     9181 ccgtgccggc atcccgctac gaagtggtgg gcccgatctg cgagagcggc gacttccttg
     9241 gccacgaccg cgacctgtcg gtggccgcgg gcgacctgct ggccatcctg tccgccgggg
     9301 cctacggcat gaccatgagc tccaactaca acacccgccc gcgggcggcg gaagtgctgg
     9361 tggacggcga cgcggtgcat ctgatccgcg aacgggaaac cctcagccac ctgctcaagg
     9421 gcgaaacgcc gctggcctaa cgcgaaggcc ggcaccacaa cacccacaaa aaaacccggc
     9481 catgccgggt ttttgtcatg aggtccgccg tacgcgctca ccccagcggc gtgaccgccc
     9541 cacgagccgc cggcgaatcg agccagcgcg acaggacgcg gtagacgtcc agatcgaagc
     9601 tgcgtggcga cggcgccgcc agcagctcgc ccagcgcgcc ctcgtcctcc acgctgccca
     9661 gcaggcacca gcgatccacc acgtgcaccg cgctgcgacc ggtctccccg tggtgttcgc
     9721 ggatcgccac cgggccgtcc catggccagc ggcgcagacg caggctctcc agcaccttca
     9781 ggagacgcgc atcgtgctca tcagggcttt cccgacccgc gcagacaccc gcgcagcgct
     9841 tcatctggtg agccatgcaa gccccttcgc cccacggctc cgtgccgatg cggcgcgggc
     9901 acagcttgta cagggccgcc agatcccgca atgcgttgtc ggcctcgcgc ttgctgcgga
     9961 aggcgccata cacggcctcc caggcggccg gatcgctgcc gttgagcgcc tcccgcagga
    10021 gcaccgggcc acgcttgcgg gcgccgtcgt agcgcagacc gaagaccgcc tcggtttcac
    10081 gcagctggcg gttctcggtg ggtttgcggg ccttgacgag gcgcgactcc aggagcagcg
    10141 caccgagctc gccggccgtg gtctgccagt ccacccgccg cacctgctgg gcgatacgcg
    10201 cctccttggc gttgcggtga tctgcagaaa aatggctcat cacccgcgag cgcaggctga
    10261 cactcttgcc gatgtagagg ggcaggtcgt tctcgccgta gaacagatac acgccaggcc
    10321 cttccggcaa gccttccagc gcgccttccg gcagcccggc tggccggctc ggcgccttca
    10381 tggccttgag gacggccgtt tccagcaccg gtgcttcgaa ctggccgcaa accagttggg
    10441 cgaactgcca caaagcgtcg gtatcgccca tggcccggtg acgggcggtg cactcgaggc
    10501 cgtggcgctc gatcagcgca tccaggccgt ggcggtgata ctggggaaac agggcgcggg
    10561 acagcttgac cgtgcacaac accggcgcgt cgaacgcctc gccgatgcgg cggaattcgt
    10621 tcttgatgaa gccgtagtca aagcgtgcgt tatgggcgac gaacacgcag tcggcgaaga
    10681 gtgcccgcac gccgtccttg agcgcgtcaa agcctggcgc ctgcgccacc atggcgtcgg
    10741 taatgccggt gaaacgctgg atggtgggcg ggatcgggat gcccggattg accagactgg
    10801 accagcgctc caccagccgg ccatcttcca cccgcaggat ggcgatctcg gtgacccggt
    10861 ccaccaccgg attggccccg gtggtttcca catcgatgaa agcgagccgg ggtgcgagca
    10921 cgacgtgtgg agcaggggga caaccgggca gggcggcgga ctcagccttc agtggagtac
    10981 gggtaaggct tctgttcaat cttcccaacg gcgaatccct cgttcagctc gggattctgc
    11041 ggacggtccc accacagggc gcgacccgcc tgctgcacag agacctcttc cgggtggttc
    11101 ttcaaccatt cgcgcataaa ttgggtgtgt tcggattcgt agatggccat atgaaatctc
    11161 ccttgaaatg acgtggattc tagcagcagt cagtggcggt ctcaggcgac cggcagcttg
    11221 gggtcaccct tcagacggcc gatgcgcacc acttccggaa tgttgcgcac gccgcggatg
    11281 accttggcca gatgcagacg gtgggtgacc tgcacggtga ggtagatcgt ggagaaggca
    11341 ccctgctccc catcggtgct gacattctgg atgttgcagt cctgctcggc gatggcattg
    11401 gccaccttgg ccagcacccc gcgcacattg tgcgtgagca gacgcagggt cacgtcgaag
    11461 aggcggtcca cgcccggttc ccactccacg tcgatccagc gctcccgctc accgcgcagc
    11521 ttgcgggcct ggggacagtc gtgcatgtgc acttccagcc cctggccctt gcggatgatg
    11581 ccgacgatgg gatcgccggg gatcggcctg cagcagcgcg ccagttgcac cgccacaccc
    11641 tcggtgccac gaatcaggat ggcaccggcc ggtttgggct tgaccacgtc ggcgcggccc
    11701 gattcgatgt cctggatctc tgccagacgc cgcgccacga tggcggacag gcgccgaccg
    11761 agcccgatgt cggtcatcag ctccttgcgg ttggccagcg tgaattcccg caggtaacgt
    11821 tcccaggcaa tcgcactgac ggaacccagg gtgagcccat gaggacgcag ggcctggttg
    11881 agcaggcgct cgccgagggt gaagaaggcc tcttcctggt gggcgttctt gaggaaatgg
    11941 cggatctgcg cccgcgcctt gccggtgcgc acataactga gccaggccgg gttggggctg
    12001 gcgtgggcgg cggtgatgat ctccacctgg tcgccattgc gcagctcggt gcgcaggggc
    12061 aggagttcgc cattgacgcg gccggccacg cagcggttac ccacgtcggt gtgcaccgcg
    12121 taggcgaagt ccaccaccgt cgcgccccgc ggcagggcaa agatcttgcc cttgggcgtg
    12181 aaggcgtaga cctcgccggg aaagagatcc accttcacgt gttcgaggaa ctcgctcgaa
    12241 tcgccggagg tggattggag ttccagcagg gactgcagcc aggtgtgggt cttggcctgc
    12301 aagtcagtga gggtcttctc gtcttccttg tacagccagt gggaggccac gccgctgtcg
    12361 gccacgtggt tcatctgggc ggtacggatc tgcacctcca acggcgtacc gaaaggcccg
    12421 atcagggtgg tgtgcaggga ctgataaccg ttcgccttgg gaatggcgat gtagtccttg
    12481 aacttgcccg gcaccggctt gtacagccca tgcagcgcac cgagggcgcg gtagcaggtc
    12541 tgcacgtcag gcacgatgac gcggaagccg tagatgtcca gcacctgcga aaaggacagg
    12601 cgcttctcgt tcatcttgcg gtagatgctg tacaggtgct tctcgcggcc ccgcacctcg
    12661 gcctggatgt tccactgggg cagccgctcc tcgatggccg cgaggatctt gccgaccacc
    12721 tcgcggcgat tgccccgcgc cgccaggatg gccttggaga gcacccggaa gcgcatcggg
    12781 tggacgtgct tgaaggccag ctcctgcaat tcccggtaga gggcgttgag acccagccgg
    12841 ttggcgatgg gggcgtggat ctcctgggtc tcgcgagcga tgcggcggcg cttgtccgga
    12901 cgcacgcagt cgagcgtacg catgttgtgg aggcggtcgg cgagcttgat caggatgacc
    12961 cgtaagtcgc tggccatggc cagcagcatc ttgcggaaat tctcggcctg cgcctcttcc
    13021 tgggactgga attcgatctt gtcgagcttg gacacgccat ccaccagttc ggcggtggtc
    13081 cttccgaaac gctctgcgat ctcggacttg gtgatgtggg tgtcctccat cacgtcgtgg
    13141 aggagcgccg cgcacaaggc ctgggcgtcc agatgccagc cggcgagaat ctcggcgacg
    13201 gcgacgggat gggagatgta aggatcgcca ctgatgcgga actgccccga atgggcgtcc
    13261 gcggcgaagt gataggccgc ctcgatgcgg cctacgtcct ccggacggag gtaggtcgcc
    13321 acctgggcac gcaggcgacc gaaatcgagc gggagcacac ttgaggccgg aggctggaaa
    13381 acgccgctgc gtccaagcgg agccgatgcg gcggattcca tgacattgcc cgctcggtga
    13441 ggatcaggcc tgaccgcggt tcaggacttc cgtccccacc ttgccggcgg cgatttcgcg
    13501 cagggcgatg acggtgggtt tgtccttgtc gtgacggtca agctccacct ggggcgtggc
    13561 accggccgtc agctgacggg cacggtaggt cgccgccagg gtgagctgga aacggttgga
    13621 aatcctcttc agacaatcat cgacggtgat acgggccatg gatcaatcct gctccagata
    13681 gtggaaaaac tcgggctggc gcgccgcctg attggcgtag cgcaagcgcg ctgcccgcac
    13741 aacggccacc agatcttcga gcgccgcgga caggtcttgg ttgattataa cgtagtcgaa
    13801 ctccccgaca tgccgcatct ctccgcgggc accgagcacg cgccgggcga tgacctcctc
    13861 gctgtcggtg ccgcggccgc gcaggcgacg ctcgagttca tccacggacg gcggcaggat
    13921 gaacaccccg acggccttgg ggaacgcctt gcgcacctgc tgggcaccct gccagtcgat
    13981 ctccagcaga atgtcccgcc ccgatgccat ctgctcctcg agccagcgcc gggatgtccc
    14041 gtaataattg ttgtggacct ccgcccattc gagaaattcc cctttgtcgc gcaaggcacg
    14101 gaacgtcggc acgtccacga agtggtaatg cacgccatgt gtctcgccgg ctcgcggcgt
    14161 ccgggtggta tacgagatgg acagactgac ctgggcatcc cgtttgagca ggccgctcac
    14221 cagagtggtc ttgccggcgc cagatggcgc cgtgacgatg aacagggttc cgggcatggg
    14281 cgtcactcga tgttctggac ctgctcgcgc atctgttcga tggccagctt gagttccacg
    14341 gcgatcgcag tcatgtcggc agccatggcc ttggagccga gcgtgttggc ctcgcgattg
    14401 agctcctgca ttaggaagtc caggcgcttg ccggcggtac cgccgctctt gaggatgcgc
    14461 tccacctcgt caaggtgggt gctcaggcgc gacagttcct ccgccacgtc aacgcgctgg
    14521 gcgaacagcc cgacttccag ccggatacgg tcgtcgtcga gggtcgcgga cgcgtcgcgc
    14581 aggcgctggg cgagcttttc ctggtattcg gcgaccagcg ccggcacacg gggctggacg
    14641 cgggccacca gatcgcgcat gtgggcgatg cgcccacgga tcatgtcggc gagcttctcg
    14701 ccctcgcgac ggcgcgaggc gacgagatcc tccagcgcag cggcgagcaa gggcgcgatc
    14761 tgcggctgga tctgttcgaa cccgacgccg tcatccccca gcatgccggg ccagcgcagg
    14821 atctcgccaa cgctcagggg cgcggcggcg ggcaactcgg cccgcaccgc ggcctcgacc
    14881 gccttcaact gaccgagcag cccaccattg agcaccagtt cggccgttgc gctctccttg
    14941 ccctgcaggt ggaagcggca ctcgaccttg ccccggctga gccgaccggt cagcgcttcc
    15001 cgcagttgag gctcggactg gcgcagctca tccgcgatgc ggaaagccag atcaagaaag
    15061 cgggaattga cgctgcgaag ctccagatgg agcgccacgc gccccagttc gcgggtctga
    15121 acggcgtaac ccgtcatgct gtgaatcatc ttgtattttc ccgaataaac gaagcaatcc
    15181 ggcgctaaac taagcacctt gccgcccaaa ggaacgaagt gtagcgcccc gcccgatggc
    15241 cactcaacaa aactgccccc tgccacccgg attccagctc gaccactacc gtgtcgagaa
    15301 gcagttgtcc ctgggcggat tttcgattgt ctacctggcc tacgaccagg acggcacgcc
    15361 ggtggccatc aaggaatatc tgccaaattc cctcgcgtta cgcgcccagg gagagatcac
    15421 cccccatgtc accgaagcgc accagagtgc cttccgctac ggcatgaagt gcttcttcga
    15481 ggaaggccgg gcgctggcca agctggtgca ccctaatgtc gtgcgggtgc tcaacttctt
    15541 tcgcgccaac ggtacggttt acatggtgat gcagttcgaa cgcggccgaa ccttgcatga
    15601 ctacgtccag aagcacaagg gcaacatccg cgagcgcttc atccgcgggg tcttcacccg
    15661 catgctcaac ggactgcgag aggtgcattc ccacaagctg ctgcacctgg acatcaagcc
    15721 gtccaacatc tatctgcggg cggacggcaa cccggtgctc cttgatttcg gcgcagcccg
    15781 tcagaccctc gcctccgaca cccccatcct caagcccatg tacacgccgg gcttcgcgtc
    15841 gcccgagcag ttcaccaacc gggaagcgct cggaccatgg agcgacatct acagcgtcgg
    15901 cgccagcatg tacgcctgtc tggcgggcac ctcgccccag cgctccgacg aacgcctgaa
    15961 caacgacacc gtcgtcccgg cctccaagct gtgggttgga cagtactcgc cgcacttcct
    16021 gtccaccatc gactggtgcc tgcaacttga tcccctgaag cgcccccaga gctgcttcgc
    16081 cctgcaaaag gtcctcgccc aacgtcaccg ggaagctcca ccggagccca agacctggct
    16141 gaccggactc ggtgaccgta taaaaacgat catgggccgt acatgagatt cacgatctac
    16201 caggaaagcc gggtcggcaa acgccgtacc aaccaggacc gggtcgccta ttcctatacc
    16261 cgcgacgcgc tcctgctcgt cctcgccgac ggcatgggcg gccacctgta cggcgaggtc
    16321 gccgcgcaga tctccgtcca gttcatcgcc cagtccttcc agcaggaagc ccgccccaag
    16381 gtcagcgacc ccatcctgtt cctgtcgcgg gtgctcagca acgcccacca cgcgatcctc
    16441 gactacgcct tcgacaagca gctgtcggac gttccccgca ccaccatcgt cgcctgcctg
    16501 atccaggacg gtgtcgccca ctgggcccac gcgggcgact cgcggctcta cctcatccgc
    16561 cgcaacaagg tgttcgccca gacccgcgac cactcccgcg tccagctcat gctcgaccag
    16621 ggcctgctcg acgctgaagc ggccgcctcg caccccggcc gcaaccgcat cttcagctgc
    16681 ctgggcggca a
//
LOCUS       DAOQPA010000082        16393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1771_length_16393_cov_15.600869, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000082 DAOQPA010000000
VERSION     DAOQPA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1771_length_16393_cov_15.600869"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PKX01_11700"
     CDS             <1..1139
                     /locus_tag="PKX01_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMZ76837.1"
                     /translation="HTIFGGKNPHPNWLVGGVPCAINLEGTGAVGAINMERLNMVSSI
                     IDRCIDFIDNVYIPDLKAIASFYKDWTYGGGISSQALLSYGDIPDKANDYTAKSLLLP
                     RGAIIGGDLGKIHEVDLKDPEQVQEYVAHSWYKYADESKGLHPFDGVTEPHFELGKAT
                     KGTKTRIENLDEAGKYSWIKAPRWRGNAMEVGPLARYVIGYAQKNPEFKEPVDKLLSD
                     LGLPLQAIFSTLGRTAARGLEASWAAHKSRYFFDKLMANLKAGDLTTAHIDKWDPSRW
                     PAEAKGAGFTEAPRGALGHWIRIKDGRIDNYQAVVPTTWNGGPRDDKGQIGAFEASLM
                     NTPVAKPDEPLEILRTLHSFDPCLACSTHVMAPDGQEMASVKVR"
     gene            1272..1976
                     /gene="cybH"
                     /locus_tag="PKX01_11705"
     CDS             1272..1976
                     /gene="cybH"
                     /locus_tag="PKX01_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HMZ76838.1"
                     /translation="MLSQQDMLEAERHGRQVRSIYVYEAPVRLWHWINALAIVVLAVT
                     GYFIGKPLPTMPGEASDNFLLGYIRFAHFAAAYVFAVGLAGRVYWAFVGNHHARQIFK
                     LPLTNAQWWSEVFFELRWYLFLEKHPKKYVGHNPMAQLMMFFFFTVLAFGMLFTGFAL
                     YSEGLGLGSWADRLFGWVIPALGGSMMTHSLHRLGMWIMMSFVVVHVYAAIREDIMSR
                     QSLISTMVSGWRMFKD"
     gene            2254..2865
                     /locus_tag="PKX01_11710"
     CDS             2254..2865
                     /locus_tag="PKX01_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HMZ76839.1"
                     /translation="MDTSPRVLVLGIGNLLWADEGFGVRCVEALAERYSVPPQVTLMD
                     GGTQGLYLLPYVEEADCLLVFDAVDYGDPPGTLRIVEGAEVPRFMGAKKMSLHQTGFQ
                     EVLASAELLGRLPRELVLIGCQPVELEDFGGSLRDAVKAQIGPALDVAAEFLRGWGAA
                     PLPRADDAPRLNAGSLGMAAYEAGRPSEQEACRFGDARFLAGD"
     gene            2868..3176
                     /gene="hypC"
                     /locus_tag="PKX01_11715"
     CDS             2868..3176
                     /gene="hypC"
                     /locus_tag="PKX01_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HMZ76840.1"
                     /translation="MCMGIPCRVVDSQGWTALCDGPAGPVSIDLALVGPQAPGTWLLT
                     FLGAAREVISAERAAQVGDALAALEAALAGETDRIDALFGDLVGREPTLPEHLRKELP
                     "
     gene            3173..3613
                     /locus_tag="PKX01_11720"
     CDS             3173..3613
                     /locus_tag="PKX01_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76841.1"
                     /translation="MTVHFAPAPSATERLEAALRRLTERHAFTRVDAAFEPPAGTAVL
                     LLSDDPQRSPEALDVIVVLPEALRALGRPLSACVADAEASAVLARRFGVARLPAVVAF
                     RDGAWLGAFSGILDWAPFVTELARLLDAPPGRPPIFVASAEARP"
     gene            3610..4458
                     /locus_tag="PKX01_11725"
     CDS             3610..4458
                     /locus_tag="PKX01_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HMZ76842.1"
                     /translation="MKPFPIPVVPVGPGSQTDEAPDYLAMPGGMATFQAPRLPEGAQA
                     ADVAHAASLLAGLVDAMAGAAFDGPPLVLPLDAETPGVREVLTQSLGFGEVSAFTVAP
                     TRLRVQETAFASLWRVLEEGEDGRLLHDRLTVCPVPPELYAAMRATARPDLVPPAVLP
                     GMMNGEALLAEVRQQAAAHRARRPAHVINLTLLPVSEADLDYLYGALGHREVSILSRG
                     YGNCRVTSTRLAHVWWVQYFNSMDTLILNTLEVVDMPDVVLAAEEDFRDSIERLREYV
                     ALLAEA"
     gene            4462..4671
                     /locus_tag="PKX01_11730"
     CDS             4462..4671
                     /locus_tag="PKX01_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMZ76843.1"
                     /translation="MFEGSFLGDAARLADTDRLECGICWTVYDPAEGDPHWGIPPGTP
                     FAQLPEHWRCPVCDAPQHKFMVLRD"
     gene            4664..5224
                     /gene="hybE"
                     /locus_tag="PKX01_11735"
     CDS             4664..5224
                     /gene="hybE"
                     /locus_tag="PKX01_11735"
                     /inference="COORDINATES: protein motif:HMM:NF023365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HMZ76844.1"
                     /translation="MTEAPRPATVPVAAAELLARIEAHYRRVAATAMADVPLCNPRLA
                     VRGVGIEDWDGQRIVALVTPWAINLLRLPGSAPLPSRGPLAKHVWRLPSGDYAFVEAG
                     DVGFGPFQSCSLFSPVLEFDSQAAACETALAALLALLRPPLAVPEAVAVADARPSGTP
                     APSRGVPPPAPAEVPSRRRWLLGRGA"
     gene            5221..6063
                     /locus_tag="PKX01_11740"
     CDS             5221..6063
                     /locus_tag="PKX01_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76845.1"
                     /translation="MSTLSLAGTLKVTARWAFGGLHDLQVDLKRPPVARALVGLTPGE
                     ALARLPVYFNLCSQAQTEAGRLALAAAGMPLGTPVESWRLWAECLHEHLWRLLLDWPR
                     VMGAVPETTAFAAWRACRAQGREALADATRELLEHRVLAGPLGGLLARLEVRLHVGQG
                     DDPVGAGLEARVQAIRDACAALAANRDYPRGSLGGEAGGRAEGWVHTARGALHHRLRL
                     AGGRVADWEIDAPTDRNFADAIALRRRLPEHLPDPREAKAALERAVLLLDPCVPYGVE
                     IDHA"
     gene            6056..6397
                     /gene="hypA"
                     /locus_tag="PKX01_11745"
     CDS             6056..6397
                     /gene="hypA"
                     /locus_tag="PKX01_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HMZ76846.1"
                     /translation="MHEMSLAEGIRQIVEDAGRSQGFGRVRAVVLEIGQLAAVEVDSL
                     RFCFDVVMKDSIAAGATLTIETLPGTGWCMQCARSVPVTALYEACPCCGSYQVQPTGG
                     TEMRVKELEID"
     gene            6403..7350
                     /gene="hypB"
                     /locus_tag="PKX01_11750"
     CDS             6403..7350
                     /gene="hypB"
                     /locus_tag="PKX01_11750"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HMZ76847.1"
                     /translation="MCTTCGCGAGETRIEGDALHEHVLADGTVLRHSHAADHPWRPRK
                     VRGVPPEASPSAADDPAVATDDGALHYGHGPAGAHAPGLSQGQMVRIEQDILGKNDGL
                     AAGNRAWLAARGILALNLVSSPGSGKTTLLVRTIEALQRRVPVCVVEGDQQTSFDAER
                     IRATGAPALQINTGKGCHLDAHMVGRALERLAPPADSVLLIENVGNLVCPAAFDLGEA
                     AKVVVLSVTEGEDKPLKYPDMFAAARLMLLNKTDLLPYLNFRVDDAIAYARRVNPALE
                     VIQLSATSGAGFDAWLAWIDARRAEASVRVRDVAAGGEG"
     gene            7355..9706
                     /gene="hypF"
                     /locus_tag="PKX01_11755"
     CDS             7355..9706
                     /gene="hypF"
                     /locus_tag="PKX01_11755"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HMZ76848.1"
                     /translation="MLRKIDPERVARRIVVRGVVQGVGFRPFVFRIARRHGLDGWVRN
                     DGTGVEIRVQGAAVRVERFIHALAEQAPPTARVSLIEPEVIAPDPHRSGFSILPSAGG
                     QIATTIGPDLAVCGDCLEELFDPQNRRYRYPFINCTHCGPRYTLTRRLPYDRATTSMA
                     GFALCPECGSEYADPANRRFHAEPNACPACGPRLSLREVHDVPALVRDPISATGQRLR
                     LGEIVAVKGLGGFHLACDAANPFAVATLRDRKRRPAQPFALMVANVASARRLARVSDE
                     EARLLESPERPVVLLAKAADFDRQFTGIAPGLDEVGLMLPATPIQFLLFHEAAGRPDG
                     TAWLQAAQDLTLVMTSANPHGEPLVRENEEAFERLEPVADEVLFHDREIVARCDDSVL
                     RVRADLPGHVQFLRRARGYTPLAWPLGRSGPAVLAFGAHLKATVCLTRGAEAFLSPHV
                     GGLENPAACAVLDETAERLQALLATVPDVVAHDLHPDYHSTRAALALAERLGVPAVAV
                     QHHHAHIAAVCAEHGIDGPVLGFAADGSGLGSDGLPWGGELLRVDGADFTRLGHLAPL
                     ALPGGDKAAREPWRVACGVLHALGLDELAARRFADQDVTRLLDLLARGLRCPPTTSLG
                     RWFDAAAGLLGVRSLSLYEGHAAMALETQARRYGAALPHPGGWHMEERPGDGSVLSLY
                     PLFPQLADETAPARGAAVFHATLVDGLVRWIGDAARAQGLRRVALGGGCLLNRLLAEG
                     LRKGLLARGLEVFEAGEFPTGDGGIALGQAWVARLAVRDGRIG"
     gene            9739..9975
                     /locus_tag="PKX01_11760"
     CDS             9739..9975
                     /locus_tag="PKX01_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HMZ76849.1"
                     /translation="MCLAIPTRVVELLPDAQALVDLGGVRKTVSLELVDDVAPGDYVI
                     VHVGYALTRLDADEAERTLALMAEAGLDVTGGGA"
     gene            9972..11123
                     /gene="hypD"
                     /locus_tag="PKX01_11765"
     CDS             9972..11123
                     /gene="hypD"
                     /locus_tag="PKX01_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HMZ76850.1"
                     /translation="MKYVDEFRDGAVARGLAAAIARAVQPDRRYAFMEFCGGHTHAIS
                     RYGVADLLPSAVRMVHGPGCPVCVLPIGRIDQAIRLALDADVTLCTYADTLRVPASDG
                     LSLMRARARGADIRMIYSAADALALAQREPQRQVVFFAIGFETTTPPTALVVRQAAAL
                     GLTNFSVLCCHVLTPAAITAILESPEVRTLHTVALDGFIGPAHVSTVIGSRPYAQFAA
                     DYRKPVVIAGFEPLDVMQAILMLVRQVNDGRAEVENEFTRAVTPDGNLKAQALMDAVF
                     EIRPDFEWRGLGVIPHSALRLRPAFADFDAERRFGLDYRPVPDHKACECGPILRGVKT
                     PTDCKLFGTVCTPESPVGSCMVSSEGACAAHYTYGRFRNIPVVAEPVAP"
     gene            11120..12166
                     /gene="hypE"
                     /locus_tag="PKX01_11770"
     CDS             11120..12166
                     /gene="hypE"
                     /locus_tag="PKX01_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HMZ76851.1"
                     /translation="MKTAYVRPLDLRHGTVDLSHGGGGRAMVQLIADVFAKALDNEWL
                     RQGDDGAVLPAPAPGERLVMATDAHVVSPLFFPGGDIGSLSVHGTINDVAMMGARPLY
                     LSASFILEEGFPLADLLRIVTNMGAAARAAGVPVVTGDTKVVEKGHGDGLYISTTGLG
                     ALPAGRRLSGAAARPGDAVLVSGTLGDHGMAILAHREHLAFESEIRSDSAALHTLAAD
                     VLAAAPDLRLLRDPTRGGLSATLNEIAQQSAVGIVIDEAAIPVDPQVAAACEFLGLDP
                     LHVANEGKLVAVCPPAQVDAALAALRAHPLGARAARIGEVIADDHRFVQMKTALGGRR
                     MVDWLAGEQLPRIC"
     gene            12179..12481
                     /locus_tag="PKX01_11775"
     CDS             12179..12481
                     /locus_tag="PKX01_11775"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HMZ76852.1"
                     /translation="MFIAMNRFRIAPGREAEFIEIWRTRESHLDSVPGFRQFHLLQGP
                     TTPEFTLFASHSVWDSAAAFEAWTRSEAFRKAHSGAGTRRDLYLGPPQFEGFEAVL"
     gene            12507..14039
                     /locus_tag="PKX01_11780"
     CDS             12507..14039
                     /locus_tag="PKX01_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ76853.1"
                     /translation="MFRDADPPARAGARPMSRTGKSARLPGVLVVDDEVRSQEALRRT
                     LDEEFEVFTASSADEAWRIMERESVQVLLCDQRMPGESGVGFLKRVRAQWPDVVRLII
                     SGYTDSEDIIAGINEAGIYQYLLKPWQPDTLLMAIRNAAELFRLQAENQRLSLELRTS
                     GAVLRKRVEMKYERVKREFGLDGLARASDSPLNPVCELIGQVAPYDLSVLISGESGTG
                     KEMLARAIHYQSRRADRAFVVENCGALPDQLLEAELFGYKRGAFTGAYEDRIGLFQQA
                     DGGTLFLDEIGETSPAFQVKLLRVLQEGEFRPLGSPRPMTVDVRVIAATNRDLETEVR
                     EGRFREDLYYRLAIVPIHVPPLRERPMDIPLLAHRLLEVSCRALGKDCDGFTPEALAC
                     LAAYRWPGNVRELQNTILRMLALTAEPQLGAHLLSPRILQAPPDPGHADLAEVGFAGT
                     LQDRVEALEARVLKETLIRLRWNKTRAANELGLSRVGLRAKLVRYGLDSDGGDHGAGS
                     GE"
     gene            14036..15031
                     /locus_tag="PKX01_11785"
     CDS             14036..15031
                     /locus_tag="PKX01_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupU protein"
                     /protein_id="HMZ76854.1"
                     /translation="MKLLWLQSGGCGGCTMSLLCSSARDLPGLLAGAGIELAWHPSLS
                     EATGDAVRALLAEFESGRTALDLLCVEGSLLTGPAGSGRFHMLSGTGRAMIDWVRSLA
                     ARARYTLAVGTCAAYGGITAAGLNPAGAVGLQYDGEQRGGLLGADYRSAAGLPVINVA
                     GCPTHPDWVTDTLIALALGRFAEADLDALGRPRFYADQLVHHGCTRNEFYEFKASAET
                     PGDLGCMMEHLGCKGTQAHADCNIRPWNGLGSCTNGGYPCISCTEPGFESPGHPFGLT
                     PKVAGIPIGLPSDMPKAWFVALAALSKSATPERVRRNSVVEAPVLAPATRRTRLK"
     gene            15078..>16393
                     /locus_tag="PKX01_11790"
     CDS             15078..>16393
                     /locus_tag="PKX01_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HMZ76855.1"
                     /translation="MSRVVVGPFNRVEGDLEVSLDFTDGRVSAAYVNSPLFRGFEQIL
                     VGRDPTDALAIVPRICGICSVSQSAAAAAALADLAGVQAPPNGRRVANLILAAENIAD
                     HFTHFYLFFMPDFARDDYRGRPWFDAAAARFKAVTGSAAADALPARAAFLQIMGCLAG
                     KWPHSMTLQAGGVARAPQTADLLRVFAALREFRTWLERHLFGDRLEHVAALDSVAALR
                     RWQATGPAAASDFGQFLRIADDLDLAALGRATDRFLSHGAYPRDAGWLFPRGVLDLPA
                     GVLQGFDPADVREDVTHAWLEPDAPRHPSEGDTQPVADKPGAYTWCKAPRWQGQVVEC
                     GALARQAVAGHPLIRELVAEAGGNVLSRVLARLLEIALVVPAMEAWLRELDTGPFRSP
                     VTLPTDGKACGLVEAARGALGHWLTVRNGHIVRYQIVAPTTWNFSPR"
BASE COUNT         2231 a         6297 c         5416 g         2449 t
ORIGIN      
        1 tccacaccat cttcggcggc aagaacccgc accccaactg gctggtgggc ggcgtgccct
       61 gcgccatcaa cctggaaggc acgggcgcgg tcggcgcgat caacatggaa cgcctgaaca
      121 tggtcagttc catcatcgac cgctgcatcg acttcatcga caacgtctac atcccggacc
      181 tcaaggccat cgccagcttc tacaaggact ggacgtacgg cggcggcatc tcgtcccagg
      241 ccctgctgtc ctacggcgac attccggaca aggccaacga ctacaccgcc aagagcctgc
      301 tcctgccccg cggcgccatc atcggcggcg acctcggcaa gatccacgag gtggatctca
      361 aggaccccga gcaggtgcag gaatacgtgg cccacagctg gtacaagtac gccgacgaat
      421 ccaaggggct gcaccccttc gacggcgtca ccgaacccca cttcgagctg ggcaaggcca
      481 ccaagggcac caagacccgg atcgagaacc tggacgaagc cggcaagtat tcctggatca
      541 aggccccgcg ctggcgcggc aacgccatgg aagtcggccc cctggcccgc tacgtgatcg
      601 gctatgccca gaagaacccg gaattcaagg agccggtgga caagctcctg tccgatctgg
      661 gcctgcccct gcaggccatc ttctcgaccc tgggccgcac cgcggcgcgg ggcctggagg
      721 catcctgggc ggcccacaag tcccgctact tcttcgacaa gctgatggcc aacctcaagg
      781 ccggcgacct gaccaccgct cacatcgaca agtgggatcc gtcccggtgg cctgccgagg
      841 ccaagggcgc cggcttcacc gaggcgcccc gcggtgccct ggggcactgg atcaggatca
      901 aggacggccg catcgacaac taccaggccg tggtgcccac cacctggaac ggcggtccgc
      961 gggacgacaa gggccagatc ggcgccttcg aggcgtccct gatgaatacg ccggtggcca
     1021 agccggacga gcccctggag atcctgcgca ccctgcattc cttcgacccc tgcctggcct
     1081 gctccaccca tgtgatggcg ccggacggtc aggaaatggc gtcggtcaag gtccgctgaa
     1141 gaggagggac gcatgcgctc cacgtccatc gcctgtgcac atcccggtcg acgcggcatc
     1201 gccggccgcc cgcgcgggca cgccgcagtg tttcgtgaat cccctgccac gccccgtcgg
     1261 gaggcctgac catgctttca caacaagaca tgctggaggc cgaacggcac ggccgtcagg
     1321 tccgctccat ctacgtgtac gaagcgccgg tgcgcctgtg gcactggatc aacgccctcg
     1381 ccatcgtggt gctggccgtc accggctatt tcatcggcaa gcccctgccg acgatgcccg
     1441 gtgaggcgtc ggacaacttc ctgctgggct acatccgctt tgcccacttc gccgcggcct
     1501 acgtgttcgc ggtggggctg gccgggcggg tgtactgggc cttcgtgggc aaccaccacg
     1561 cccgtcagat cttcaagctg cccctcacca acgcccagtg gtggagcgaa gtcttcttcg
     1621 aactgcgctg gtatctcttt ctggaaaagc acccgaaaaa gtacgtgggc cacaacccca
     1681 tggcccagct gatgatgttc ttcttcttca ccgtgctggc cttcgggatg ctcttcaccg
     1741 gcttcgccct ctacagcgaa gggctgggcc tcggctcctg ggccgaccgc ctgttcggct
     1801 gggtgatccc cgccctcggc ggctccatga tgacccacag cctgcaccgc ctgggcatgt
     1861 ggatcatgat gagcttcgtc gtggtgcatg tgtacgccgc catccgcgag gacatcatga
     1921 gccgccagag cctgatctcc acgatggtgt ccggctggcg catgttcaag gattgacgca
     1981 tcggggcgcc tccgccccgc gtatcccttt ccccgccgcg gccgggtcgc ctcccttctt
     2041 gggcggtccg gccgcaactt catgggtgcc tgcatgattc gatgcttccg ttttcagagc
     2101 aaactgtaag tctggtttcc gcttgcaggg gcgttgaccg cccatgacga cgggaagggg
     2161 ctgtatctgc tgccattttt ccgccgaaat ggcagggcgg tcgcctggca cgggtcttgt
     2221 taaggagtca tgcctttcgg actccggatt cccatggaca cctctccccg cgttctcgtg
     2281 ctcggcatcg gcaaccttct gtgggccgac gaaggctttg gcgtgcgctg cgtcgaagcc
     2341 ctcgccgagc gctacagcgt gccgccccag gtcaccctga tggacggggg cacccagggc
     2401 ctctacctgc tgccttacgt ggaggaggcc gactgcctgc tggtcttcga cgcggtcgat
     2461 tacggcgacc cgccgggcac cctgcgcatc gtcgaaggcg ccgaggtgcc gcgcttcatg
     2521 ggcgccaaga agatgagcct gcaccagacc ggcttccagg aagtgctggc ctccgccgag
     2581 ctgctcggcc ggctgccgcg ggaactggtc ctgatcggct gccagccggt cgagctggaa
     2641 gacttcggcg gcagcctgcg ggacgcggtc aaggcccaga tcggccccgc cctcgacgtg
     2701 gcggcggagt tcctgcgcgg gtggggcgcc gcgccgctgc cacgtgccga cgacgccccg
     2761 cgcctcaacg ccggcagcct gggcatggcc gcctacgaag ccggccgtcc cagcgaacag
     2821 gaggcgtgcc gcttcggcga cgcgcgcttc ctggcgggag actgaacatg tgcatgggca
     2881 tcccctgccg cgtcgtggac agccagggct ggaccgccct gtgcgacggc ccggccgggc
     2941 cggtcagcat cgacctggcc ctcgtcggcc cccaggcgcc cggcacctgg ctgctcacct
     3001 tcctgggcgc cgcgcgggaa gtcatcagcg ccgagcgggc tgcccaggtg ggcgacgccc
     3061 ttgccgcgct ggaggcggcc ctcgccggcg agaccgaccg catcgacgcc ctctttggcg
     3121 acctggtggg gcgcgaaccc actttgcccg aacacctgcg aaaggaattg ccgtgaccgt
     3181 ccattttgcc ccggcgccca gcgccacgga gcgcctggag gcggccctca ggcgcctcac
     3241 cgagcgccac gccttcaccc gcgtggatgc ggccttcgaa ccgcctgccg gcacggcggt
     3301 gctgctcctc agcgacgacc cccagcgcag tccggaagcc ctcgacgtca tcgtcgtgct
     3361 gccggaagcc ctgcgcgcgc tgggccggcc attgtccgcc tgcgtcgccg acgcagaggc
     3421 cagcgccgtg ctggcccgcc gtttcggcgt cgcccgtctg ccggccgtcg tggccttccg
     3481 cgacggggcc tggctgggcg ccttctccgg catcctggac tgggcaccct tcgtcacgga
     3541 actggcccgg ctgctcgacg cgccgccggg ccgtccgccc atttttgtcg cgtccgccga
     3601 ggcccgtcca tgaagccctt cccgatcccc gtggtgcccg ttggccccgg cagccagacc
     3661 gacgaggccc cggactacct ggccatgccc ggtggcatgg ccacctttca ggccccgcgc
     3721 ctgcccgaag gcgcccaggc ggcggacgtg gcccatgccg cgtccctgct ggccgggctg
     3781 gtggacgcca tggccggtgc cgccttcgac ggcccgcctc tggttctgcc gctggatgcc
     3841 gagacgccgg gcgtgcggga ggtattgacc cagtccctgg gcttcggcga agtctctgcc
     3901 ttcaccgtcg caccgacccg cctgcgcgtg caggagaccg ccttcgcgtc cctgtggcgg
     3961 gtgctggagg agggcgagga cggccgcctg ctccacgacc gcctcacggt ctgcccggtg
     4021 ccgccggagc tctacgccgc catgcgtgcc acggcgcggc cggatctcgt tccgcctgct
     4081 gtgctgcccg gcatgatgaa cggcgaggcc ctcctcgccg aagtgcgcca gcaggccgcg
     4141 gcgcaccgcg ccagacgtcc cgcccacgtc atcaacctga ccctgctgcc ggtcagcgag
     4201 gccgatctgg actaccttta cggcgccctc ggccaccggg aggtgtccat cctgtcccgc
     4261 ggctacggca actgccgagt caccagcacg cgcctcgccc acgtgtggtg ggtgcagtac
     4321 ttcaacagca tggataccct gatcctcaac accctggagg tggtggacat gcccgacgtg
     4381 gtgctcgccg cagaagagga cttccgcgac agcatcgaac gcctgcggga atacgtcgcc
     4441 ctgctggcgg aggcctgagc catgttcgaa ggcagcttcc tcggcgacgc cgccaggctg
     4501 gcggatacgg accgcctcga atgtggcatc tgctggacgg tctacgaccc agcggagggc
     4561 gatccccact ggggcatccc gcctggcaca cccttcgcgc agctccccga gcactggcgc
     4621 tgtcccgtct gcgacgcgcc ccagcacaag ttcatggtgc tgcgtgactg aggcgccgcg
     4681 gcccgcgacg gtcccggtgg ccgcggcgga gctgctcgcc cgcatcgaag cccattaccg
     4741 ccgcgtggct gccaccgcca tggcggacgt gcccctgtgc aacccgcgcc tcgccgtgcg
     4801 gggggtgggc atcgaggact gggacgggca gcggatcgtc gccctggtga cgccctgggc
     4861 gatcaacctg ctgcgcctgc ccggcagcgc gccgctgcct tcccgagggc ccctcgccaa
     4921 gcacgtctgg cgcctgccca gcggcgacta cgcttttgtc gaagccggtg acgtgggctt
     4981 cggccccttc cagagctgct ccctgttctc gccggtgctg gagttcgaca gtcaggccgc
     5041 cgcctgtgag accgccctgg ccgcgctgct ggcgttgctg cgtccgcccc tggcggtacc
     5101 ggaagccgtc gcggtcgccg acgcacgccc gagcggcacg cctgccccgt cgcggggcgt
     5161 tccgccgccc gcgcccgcgg aagtcccgtc ccgccgtcgc tggctgctgg ggcgcggcgc
     5221 atgagcaccc tgtccctcgc cggcacgctc aaggtaacgg cccgctgggc ctttggcggg
     5281 ctccacgacc tgcaggtgga cctcaagcgg ccgcctgtcg cccgcgcctt ggtggggctc
     5341 accccgggcg aggcgctggc ccgtctgccg gtgtatttca acctgtgcag ccaggcccag
     5401 accgaagccg ggcgcctggc cctggccgcg gcgggcatgc ccctcggcac gccggtcgag
     5461 agctggcggc tgtgggcgga atgcctgcac gaacacctgt ggcgtctgct cctggactgg
     5521 ccgcgggtca tgggcgcggt gcccgagacg accgcctttg ccgcctggcg cgcgtgccgc
     5581 gcccaggggc gcgaggcgct cgccgacgcc acccgcgagc tgctcgagca ccgggtgctg
     5641 gccggccccc tgggcggcct gctggcgcgt ctggaggtgc ggctccacgt ggggcagggc
     5701 gacgatccgg tcggcgccgg gctggaggcc cgcgtgcagg cgatccgtga cgcctgcgcc
     5761 gcactggccg ccaaccggga ctacccccgt ggtagccttg gcggtgaggc cggcgggcgg
     5821 gcggaaggct gggtgcacac cgcccgcggc gcgctgcacc accggctcag gctggcgggc
     5881 ggccgggtgg ccgactggga gatcgacgcg ccgactgacc gcaacttcgc cgacgccatc
     5941 gccctgcggc gacgcctgcc ggagcacctg ccggatcccc gggaggcgaa ggcggcgttg
     6001 gagcgggccg tgctgctgct cgacccctgc gtgccctacg gggtggagat cgaccatgca
     6061 tgaaatgtcc ctcgcggagg gcatccgcca gatcgtcgag gacgctggcc gcagccaggg
     6121 cttcggccgg gtccgtgcgg tggtgctgga gatcggccag ctcgcggcgg tcgaagtgga
     6181 cagcctgcgc ttctgcttcg acgtggtgat gaaggattcc atcgccgcgg gcgcgacgct
     6241 gacgatcgag accctgccgg gcaccggctg gtgcatgcag tgcgcccgga gtgtgccggt
     6301 gacggccctc tacgaggcct gtccgtgctg cggcagctac caggtgcagc ccaccggcgg
     6361 caccgagatg cgggtcaagg aactggaaat cgactgaaga tcatgtgtac gacatgcggc
     6421 tgcggggccg gagaaacccg tatcgaaggc gacgcgctcc acgaacacgt gctggcggac
     6481 ggcaccgtgc tacgccacag tcacgccgcc gaccatccct ggcggccgcg caaggtccgc
     6541 ggcgtgccgc ccgaggccag cccttcggcg gccgacgatc cggcggtggc caccgatgac
     6601 ggcgccctgc actacggcca cggaccggcc ggcgcccacg cgcccggcct cagccagggc
     6661 cagatggtgc gcatcgagca ggacatcctc ggcaagaacg acggcctcgc cgccggcaac
     6721 cgggcctggc tggcggcgcg gggcatcctg gccctgaacc tggtgtccag ccccggctcc
     6781 ggcaagacca ccctgctggt gcgcaccatc gaggcgctcc agcggcgcgt gccggtgtgc
     6841 gtcgtggaag gcgaccagca gaccagcttc gacgccgaac gcatccgcgc caccggcgcg
     6901 ccggccctgc agatcaacac cggcaagggc tgtcacctgg acgctcacat ggtcggccgc
     6961 gcgctcgagc gcctcgcgcc ccccgccgac agcgtgctgc tcatcgagaa cgtgggcaat
     7021 ctggtgtgtc cggcagcctt cgacctgggc gaggcggcca aggtggtggt gctgtccgtc
     7081 accgaagggg aggacaagcc cctcaagtac ccggacatgt ttgccgccgc ccgcctcatg
     7141 ctcctcaaca agaccgacct gctgccctac ctgaacttcc gcgtggacga cgccatcgcc
     7201 tacgcccgcc gggtcaatcc ggccctggag gtgatccagc tgtcggccac cagcggcgcg
     7261 ggcttcgacg cctggctggc gtggatcgac gcgcgtcgcg cagaggcgtc cgtgcgggtg
     7321 cgggacgtcg ccgcgggcgg ggagggctga gagcatgctg cgcaagatcg atccggagcg
     7381 ggttgcccgc cgcatcgtcg tccgcggtgt agtgcagggc gtcggcttcc ggcccttcgt
     7441 gttccgcatc gcgcggcgcc atgggctcga cggctgggtg cgcaacgacg gcaccggcgt
     7501 ggaaatccgc gtgcagggcg ccgccgtacg ggtcgagcgc ttcatccacg cccttgctga
     7561 gcaggcgccg cccaccgccc gcgtgagcct catcgaaccc gaggtgatcg cgccggaccc
     7621 gcatcgcagc ggtttctcca tcctgcccag cgccggcgga cagatcgcca ccaccatcgg
     7681 acccgacctg gccgtgtgcg gcgactgcct cgaagagctc ttcgacccgc agaaccgccg
     7741 ctaccgctac cccttcatca actgcaccca ctgcggaccg cgctacaccc tcacccggcg
     7801 tctgccctac gaccgggcca ccaccagcat ggccggcttc gccctgtgtc ccgaatgcgg
     7861 cagcgaatac gccgacccgg ccaaccgccg cttccacgcc gaacccaacg cctgcccggc
     7921 ctgcggcccg cgcctgtccc tgcgggaggt gcacgacgtg ccggccctgg tccgcgaccc
     7981 catcagcgcc accggccagc gcctgcgcct gggcgagatc gtcgccgtca agggcctggg
     8041 gggcttccac ctggcctgcg acgcggccaa tcccttcgcc gtcgccaccc tgcgcgaccg
     8101 caagcggcgg cccgcccagc cttttgccct gatggtcgcc aatgtggcca gcgcgcgccg
     8161 cctggcgcgg gtgtcggacg aggaggcgcg gctgctggag agtcccgagc ggcccgtcgt
     8221 gctgctcgcc aaggcggcgg atttcgatcg ccagttcacc ggcatcgcgc ccggcctgga
     8281 cgaagtgggg ctgatgctgc ccgccacgcc gatccagttc ctgctcttcc acgaagccgc
     8341 cggccggccc gatggcaccg cctggctgca ggcggcccag gatctgaccc tggtgatgac
     8401 cagtgccaat ccccacggcg aacccctggt gcgggagaac gaggaggcct tcgagcggct
     8461 cgagccggtg gccgacgagg tgctgttcca cgaccgggag atcgtcgccc gctgcgacga
     8521 ttccgtgctg cgggtccgtg ccgacctgcc cggccacgtc cagttcctgc gccgtgcccg
     8581 cgggtacacg ccgctcgcct ggcccctcgg ccgcagcggc ccggcggtgc tggccttcgg
     8641 cgcccacctc aaggccaccg tgtgcctcac ccgcggcgcc gaggccttcc tgtcgcccca
     8701 cgtcggcggt cttgaaaacc cggccgcctg tgcggtgctg gacgagacgg ccgaacgtct
     8761 gcaggccctg ctcgccaccg tgcccgacgt ggtcgcccac gacctccatc ccgactacca
     8821 ctccacccgc gctgccctgg ccctggccga gcgtctcggc gtgcctgccg tcgccgtgca
     8881 gcaccaccac gcccacatcg ccgcggtgtg tgccgagcac ggcatcgacg gtccggtgct
     8941 gggcttcgcc gccgacggta gcggcctggg cagcgacggc ctgccctggg ggggcgagct
     9001 tctgcgggtg gacggcgccg acttcacgcg cctgggtcac ctcgccccgc tggccctgcc
     9061 cggcggcgac aaggctgcgc gtgagccctg gcgcgtcgcc tgcggcgtgc tccacgccct
     9121 gggcctggac gaactcgccg cccggcggtt tgccgaccag gacgtgacgc gtctgctcga
     9181 tctcctcgcc cgcggcctgc gctgtccgcc caccacgtcc ctgggccgct ggttcgacgc
     9241 cgccgccggc ctgctggggg tgcgcagcct gtccctctac gaaggccatg ccgcgatggc
     9301 gctggaaacg caggcgcgcc gttacggcgc ggcgctgccc caccccggcg gctggcacat
     9361 ggaggagcgg cccggtgacg gcagcgtgct gagcctctac cccctgttcc cgcagctcgc
     9421 cgacgagacc gcgcccgccc gcggtgcggc ggtcttccac gccaccctgg tggatggcct
     9481 ggtgcgctgg atcggcgatg ccgcccgggc ccagggcctg cgccgggtcg ctctgggggg
     9541 cggctgcctg ctcaaccgcc tgctcgccga aggcctgcgc aagggcctcc tggcccgcgg
     9601 gctggaggtc ttcgaggcgg gagaattccc caccggcgac ggcggtatcg ccctcggcca
     9661 ggcctgggtg gcacgtctcg ccgtgcggga cggacggatc ggatgaggtc cgcgatgatt
     9721 cctgagggag tgtgaagcat gtgtctggcc atcccgaccc gcgtggtcga actcctgccc
     9781 gacgcccagg ccctggtgga cctgggcggc gtgcgcaaga ccgtctccct tgaactggtg
     9841 gacgatgtcg cgccgggcga ttacgtgatc gtccacgtgg gctacgccct gacccgcctc
     9901 gacgcggacg aagccgagcg caccctggcc ctgatggccg aggccggcct cgacgtgacg
     9961 gggggcggcg catgaagtac gtggacgagt tccgcgacgg cgccgtggcc cgcggcctgg
    10021 ccgccgccat cgcccgcgcc gtgcagcccg atcggcgcta tgccttcatg gagttctgcg
    10081 gcgggcacac ccacgccatc tcccgctacg gcgtggccga tctgctgccg tcagcggtgc
    10141 gcatggtgca tggtcccggc tgtccggtgt gcgtgctgcc catcggccgt atcgaccagg
    10201 ccatccgcct cgccctcgac gccgacgtga ccctgtgcac ctacgccgac accctgcgtg
    10261 tgccggcctc cgacggcctc tccctgatgc gcgcgcgggc ccgcggagcg gacattcgca
    10321 tgatctattc cgccgccgac gccctggccc tggcccagcg ggaaccccag cgccaggtgg
    10381 tgttcttcgc catcggtttc gagaccacca cgccgcccac cgccctggtg gtgcgccagg
    10441 cggcggccct cgggctcacc aacttcagcg tgctgtgctg ccatgtgctc acgcccgccg
    10501 ccatcaccgc catcctcgaa tcgcccgagg tgcgcaccct gcacacggtg gccctggacg
    10561 gcttcatcgg tcccgcccat gtgagcacgg tgatcggcag ccggccctat gcccagttcg
    10621 ccgccgacta ccgcaagccg gtggtcatcg ccggcttcga gcccctggac gtgatgcagg
    10681 ccatcctgat gctggtccgc caggtgaacg acggccgcgc cgaggtggag aacgagttca
    10741 cccgcgccgt cacgccggac ggcaacctca aggcccaggc gctgatggat gccgtcttcg
    10801 agatccgccc ggatttcgag tggcgcggcc tgggcgtcat cccccattcc gccctgcgcc
    10861 tgcgcccggc ctttgccgac ttcgatgcgg agcggcgctt cggcctggac taccggccgg
    10921 tgcccgacca caaggcctgc gagtgcggcc ccatcctgcg gggcgtgaag acgccgacgg
    10981 actgcaagct gttcggcacc gtgtgcacgc ccgagtcgcc cgtgggctcg tgcatggtgt
    11041 cttccgaagg cgcctgcgcc gcccactaca cctatggccg tttccggaat atcccggtgg
    11101 tcgccgaacc cgtcgcccca tgaagaccgc ctacgtccgc cccctcgatc tgcgccacgg
    11161 caccgtcgac ctgtcccacg gcggcggcgg ccgtgccatg gtccagctca tcgccgacgt
    11221 cttcgccaag gccctcgaca acgaatggct gcgccagggt gacgacggtg ccgtgctgcc
    11281 ggcgcccgcg cccggcgagc gcctggtgat ggccaccgac gcccacgtgg tgtcgcccct
    11341 gttctttccc ggcggcgaca ttggcagcct cagcgtgcat ggcaccatca acgacgtggc
    11401 catgatgggc gcgcggcccc tgtacctgtc cgccagcttc atcctcgaag aaggcttccc
    11461 gctggccgac ctgctgcgca tcgtcacgaa catgggcgca gccgcgcgtg ccgccggcgt
    11521 gcccgtcgtc accggcgaca ccaaggtggt cgagaaaggc cacggcgacg ggctctacat
    11581 cagcaccacc ggcctcggcg cgctgccggc ggggcggcgc ctgtccggcg ctgcagcgcg
    11641 gccgggcgac gcggtgctgg tctccggcac cctcggcgac cacggcatgg ccatcctcgc
    11701 ccaccgcgag cacctggcct tcgagtccga gatccgctcc gacagcgccg ccctgcacac
    11761 cctcgccgct gacgttctgg ctgccgcacc ggatctgcgc ctgctgcgcg acccgacccg
    11821 cggcgggctg tccgccaccc tcaacgagat cgcccagcag tccgctgtcg gcatcgtcat
    11881 cgacgaggcg gcgatccccg tggaccccca ggtggccgcc gcctgcgaat tcctcggcct
    11941 cgacccgctg cacgtggcca acgaaggcaa gctcgtcgcc gtctgtccgc cggcccaggt
    12001 cgacgccgcc ctggccgccc tgcgcgccca ccccctcggc gcccgcgccg cgcgcatcgg
    12061 cgaggtgatc gccgacgacc accgcttcgt gcagatgaag accgccctcg gcggccgccg
    12121 catggtggac tggctcgccg gcgaacaact gccccgcatc tgctgaccgg agatccccat
    12181 gttcatcgcc atgaaccgct tccgcatcgc ccccggccgg gaggccgaat tcatcgagat
    12241 ctggcgcacc cgcgaatccc acctggacag cgtgcccggc ttccgccagt tccacctgct
    12301 gcaaggcccc accaccccgg agttcaccct cttcgcgtcc cattccgtgt gggactcggc
    12361 cgccgccttc gaggcctgga cccgctccga ggccttccgc aaggcccatt ccggcgccgg
    12421 cacgcgccgt gacctgtacc tcggcccgcc ccagttcgag ggcttcgagg cggtgctgta
    12481 aggccggaca ccgcgaggat gagaccgtgt tccgcgatgc cgacccgccc gcccgcgccg
    12541 gggcccgtcc gatgagccgc accggcaagt ccgcccgcct gcccggcgtg ctggtggtgg
    12601 acgacgaggt ccgctcccag gaagccctgc gccgcaccct cgacgaggaa ttcgaggtct
    12661 tcaccgcctc ctcggccgac gaggcctggc gcatcatgga gcgggagagc gtgcaggtgc
    12721 tcctgtgcga ccagcgcatg cccggcgaat ccggcgtcgg cttcctcaag cgcgtgcgtg
    12781 cccagtggcc cgacgtggtg cgcctgatca tctccggcta cacggactcc gaggacatca
    12841 tcgccggcat caacgaagcc ggcatctacc agtacctcct caagccctgg cagcccgaca
    12901 cgctgctgat ggccatccgc aacgccgccg aactcttccg cctgcaggcc gagaaccagc
    12961 gcctgagcct ggagctgcgc accagcggcg cggtgctgcg caagcgcgtc gagatgaagt
    13021 acgagcgggt caagcgtgaa tttggtctgg acggcctcgc ccgcgcgtcg gacagccccc
    13081 tcaacccggt gtgcgagctg atcggccagg tggcgcccta cgacctctcc gtgctcatct
    13141 ccggcgagtc cggcaccggc aaggagatgc tcgcccgcgc catccactac cagagccgcc
    13201 gtgccgaccg ggcctttgtc gtggagaact gcggcgcgct gcccgaccag ctgctggagg
    13261 cggagctctt cggctacaag cgcggcgcct tcaccggcgc ctacgaggac cgcatcggcc
    13321 tgttccagca ggccgacggc gggacgctct ttctcgacga gatcggcgag acctcgccgg
    13381 ccttccaggt caagctgctg cgggtactcc aggagggcga gttccgcccc ctcggcagcc
    13441 cgcggcccat gaccgtggac gtgcgcgtca tcgccgccac caaccgggac ctggaaaccg
    13501 aggtgcgcga agggcgcttc cgcgaagacc tgtactaccg cctcgccatc gtgcccatcc
    13561 acgtgccacc cctgcgcgag cggcccatgg acatcccgct cctcgcccac cgcctgctgg
    13621 aggtgagctg ccgcgccctc ggcaaggact gcgacggctt caccccggaa gccctggcct
    13681 gcctcgccgc ctaccgctgg ccgggcaatg tgcgcgagct ccagaacacc atcctgcgca
    13741 tgctggccct gaccgccgaa ccgcaactgg gcgcccacct gctgtcgccg cgcatcctcc
    13801 aggcgccgcc cgatccaggc catgccgatc ttgccgaagt gggtttcgcc ggcaccctgc
    13861 aggaccgggt cgaggcgctg gaggcgcgcg tcctcaagga aaccctgatc cgcctgcgtt
    13921 ggaacaagac ccgcgccgcc aacgagctgg gcctgtcgcg ggtgggtttg cgcgccaagc
    13981 tggtccgcta cggactcgac agcgatggcg gcgaccacgg cgcggggagc ggcgagtgaa
    14041 gctgttgtgg ctccagtccg gcggctgcgg cggctgcacc atgtccctgc tgtgctcgtc
    14101 ggcgcgggac ctgccgggcc tgctggccgg cgccggcatc gagctggcct ggcatccgtc
    14161 cctctccgaa gccaccggcg acgcggtgcg tgccctgctg gcggagttcg aatccggccg
    14221 cacggcgctg gacctgctct gcgtggaagg ctccctgctc accggcccgg ccggcagcgg
    14281 gcgcttccac atgctgtccg gcaccggccg ggccatgatc gactgggtgc gcagcctcgc
    14341 cgcgcgggcc cgctacacgc tggcggtggg cacctgcgcg gcctacggcg gcatcaccgc
    14401 ggcgggcctc aacccggccg gcgccgtggg cctgcaatac gacggcgaac agcgcggcgg
    14461 cctgctcggc gcggactacc gctccgccgc cggcctgccc gtcatcaacg tggcgggctg
    14521 cccgacccat cccgactggg tcaccgacac cctcatcgcc ctggccctcg gccgcttcgc
    14581 cgaggccgac ctggacgccc tcggccggcc gcgcttctac gcggaccagc tggtccatca
    14641 cggctgcacc cgcaacgagt tctacgagtt caaggccagc gccgaaaccc ccggcgatct
    14701 gggctgcatg atggaacacc tgggctgcaa gggcacccag gcccacgccg actgcaacat
    14761 ccgcccatgg aacggcctgg gttcgtgcac caacggcggc tacccgtgca tcagctgcac
    14821 cgagccgggc ttcgaatcgc cgggccatcc cttcgggctg acgcccaagg tggccggcat
    14881 tcccatcggt ctgccctccg acatgcccaa ggcctggttc gtggccctgg ccgccctctc
    14941 caaatccgcc acgccggagc gggtgcggcg caactcggtg gtcgaagccc cggtgctggc
    15001 cccggcgacc cgacggacgc gcctcaaatg agcgtctgcc ctggccgcag caccgtgcag
    15061 actgtcggcg gacgcccatg agccgcgtcg tcgtcggccc cttcaaccgc gtcgaaggcg
    15121 acctggaggt cagcctcgac ttcaccgacg ggcgggtgag cgccgcctac gtcaattcgc
    15181 ccctgttccg cggcttcgag cagatcctcg tcggccgcga ccccaccgac gccctcgcca
    15241 tcgtgccgcg catctgcggc atctgctccg tgtcccagag cgccgccgcc gccgccgcgc
    15301 tggccgacct ggccggcgtg caggccccgc ccaacggccg ccgcgtggcc aacctgatcc
    15361 tcgccgccga gaacatcgcc gaccacttca cccacttcta cctcttcttc atgccggatt
    15421 tcgcccgcga cgactaccgc gggcggccct ggttcgacgc tgcggcggcc cgcttcaagg
    15481 ccgtcaccgg cagcgccgcg gccgacgccc tgccggcgcg ggcggccttc ctgcagatca
    15541 tgggctgcct cgccggcaag tggccccaca gcatgaccct gcaagccggc ggcgtcgccc
    15601 gcgcgcccca gaccgccgac ctgctgcggg tcttcgcggc cctgcgggaa ttccgcacct
    15661 ggctggagcg ccatctcttc ggcgaccgtc ttgagcacgt cgccgccctc gattccgtgg
    15721 ccgccctgcg ccgctggcag gccaccggcc ccgccgcggc cagcgatttc gggcagttcc
    15781 tgcgcatcgc cgacgacctg gatctggccg ccctcggccg cgccaccgac cgcttcctca
    15841 gccacggcgc ctacccccgg gacgccggct ggctcttccc ccgcggcgtg ctggacctgc
    15901 ccgccggcgt cctgcagggc ttcgatcccg ccgacgtgcg cgaggacgtc acccacgcct
    15961 ggctggagcc cgacgccccg cgtcaccctt ccgaaggcga cacccagccc gtcgccgaca
    16021 aacccggcgc ctacacctgg tgcaaggccc cccgctggca ggggcaggtg gtggaatgcg
    16081 gcgccctcgc acgccaggcc gtcgccggcc atccgctgat ccgcgaactc gtcgccgaag
    16141 ccggcggcaa cgtcctcagc cgcgtcctcg cccgcctgct ggagatcgcc ctcgtcgtgc
    16201 ccgccatgga agcctggctg cgcgaactcg acaccggccc cttccgcagc cccgtcaccc
    16261 ttcccaccga cggcaaggcc tgcggcctcg tcgaagccgc ccgcggcgcc ctcggccact
    16321 ggctcaccgt ccgcaacggc cacatcgtcc gctaccagat cgtcgccccc accacctgga
    16381 acttctcccc ccg
//
LOCUS       DAOQPA010000083        15941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1849_length_15941_cov_18.466134, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000083 DAOQPA010000000
VERSION     DAOQPA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15941
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_15941_cov_18.466134"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKX01_11795"
     CDS             <1..1268
                     /locus_tag="PKX01_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ76856.1"
                     /translation="KAVAVCMNNHQCQRDGKGVMCPSYRITHDPAHSTGARVKAFKAA
                     LNGELGGAPGDNPFAHPDLAAAMDLCVSCKGCKKECPSAVDMTLIKTEYLAQRNAVQG
                     VSRRARLFGALPRWLHRYRGVLAALVRLRNASPWVAKKAEGWLGIAAQRSIPSITGHG
                     VADRPGPVDAPRGRVALFVDTFTHYYTPEVAEAAREVLEAGGYAVQVLRPAAGDDEPE
                     RPLCCGRSYLSQGLVDEAREEGRRTMAALVPLLAEGVPVVGLEPSCLLALRDEFYSLS
                     LGPEVGQLGKLAFLFEEFLMREGNKGLKLPLKPLPGGRAVVHGHCHQKAFGAMKATRK
                     VLGWIPGFEFDIVDASCCGMSGSFGLEAEHYAASLKMGELSLLPAVRQAAPDAPVIAD
                     GFSCRHQILDGTGREAEHVAVVLRRALDV"
     gene            1347..1598
                     /locus_tag="PKX01_11800"
     CDS             1347..1598
                     /locus_tag="PKX01_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ76857.1"
                     /translation="MDAITYSTARANLAKTMDRVCEDHAPLIITRNGEQSVVMLSLED
                     YNALEETAYLLRSPANARRLLSAVEQLAAGQGVERDVSL"
     gene            1595..1849
                     /locus_tag="PKX01_11805"
     CDS             1595..1849
                     /locus_tag="PKX01_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HMZ76858.1"
                     /translation="MRLIFSDAAWEDYLYWQHQDRKMVERINTLIQEVKREPFSGRGK
                     PEPLKHALSGFWSRRITDEHRMVYRIEGDAVLLAQLRYHY"
     gene            complement(2023..2415)
                     /locus_tag="PKX01_11810"
     CDS             complement(2023..2415)
                     /locus_tag="PKX01_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76859.1"
                     /translation="MKVAIATQNFTIVSGHAGQSRQWLVYDLGEHHSSRLLPAPQRIE
                     LAEDEVMHVFKDDRPHPLDGVDVVVAASAGDGFVRHMRHRRADVLLTRESDPALAITH
                     ILAGEALPDPRFDVTTTLCKIRDLFSRY"
     gene            complement(2412..3281)
                     /gene="modD"
                     /locus_tag="PKX01_11815"
     CDS             complement(2412..3281)
                     /gene="modD"
                     /locus_tag="PKX01_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HMZ76860.1"
                     /translation="MNPYTLPDTDLEALLQDDTPCGDATTHTLGIGSRRGRMVLRARY
                     AMTLCGSEEAARMGVLRGLNVDGPVLPSGSHLDAGTAFLSLSGPADALHAVWKTAQTL
                     VEYLSGIATCAAELLAAARRAHPDAGVVCTRKNFPGTKRAALKAILCAGASPHRISLS
                     ETLLIFPEHRAFLPPDESPAATLAHLRRQWPERPVVVEVAQAADALAWAAAGVDILQL
                     EKMSPDTVAALHAQISAGPGHPRLAAAGGITPANAEAYVRAGAHILVTSAPYFAPPKD
                     VAVTLTPADEPRS"
     gene            complement(3307..3849)
                     /locus_tag="PKX01_11820"
     CDS             complement(3307..3849)
                     /locus_tag="PKX01_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HMZ76861.1"
                     /translation="MPIGIFFGTDTGRTRLIAKQIAKKLGDAAAAPVNIGRATLDDFL
                     AYDCWILGSPTLGDGELPGQSVGLSQPSWEEFLPQLDGVDLRGRTAAIFGLGDQKKYP
                     DEFVDAISLLHDALLARGVRIVGRWPVAGYEFNQSKAQDGEHFLGLALDQHHQPVLTE
                     ARLDAWLAQIRAEMAAPVSA"
     gene            complement(3854..4123)
                     /locus_tag="PKX01_11825"
     CDS             complement(3854..4123)
                     /locus_tag="PKX01_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HMZ76862.1"
                     /translation="MSTVQYEIGDMVFAAEDLLNDGGMPGVTEEEGLIVAEGTRGVVV
                     HFGYAELDESKEIYLVRFENGPDGTLGAPVGCLPDELTQDVPVRN"
     gene            complement(4128..4457)
                     /locus_tag="PKX01_11830"
     CDS             complement(4128..4457)
                     /locus_tag="PKX01_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HMZ76863.1"
                     /translation="MPRPKKHVLVCVQGRPPGHPRGSCQANGCAPVWQAFSDEFTTRN
                     LWASGFQLTNTGCLGPCNLGPTAIVYPEGTMYVGIKPEDVATIIDEHLMFDQPVERLK
                     APADVWE"
     gene            complement(4582..6216)
                     /gene="nifA"
                     /locus_tag="PKX01_11835"
     CDS             complement(4582..6216)
                     /gene="nifA"
                     /locus_tag="PKX01_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HMZ76864.1"
                     /translation="MQERPKLLDECPPVGGFCRTHELNIQLLAALYAVSRVLSRSLAF
                     GETLRDVLRVLDEEAGLGLGLIAVVDAQSGTLGTHVIHHPNVVGDVSYQPGEGVLGTI
                     LEKPRTIMLQRIADEPRFLNRLGIYQPGLPFIAVPIKAGSTLQGVLAVQPERPDDGLL
                     EERAQFVEMVANLIGQNLRLSLEVEQEKSSLVEERDQLRRTVRHQYGFDSMVGRSAVM
                     RRVFDQARMVAKWNTTVLIRGETGTGKELIANAIHYNSPRARNAFVRLNCAALPENLL
                     ESELFGHERGAFTGAVEARKGRFEQAHGGTLFLDEIGEISATFQAKMLRVLQEGEFER
                     VGGSRTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERVEDILEI
                     ARHLLNKIGGEQKRKLALTDMANRRLASYDWPGNVRELENCLERAAVLSEDGRIDAEL
                     IRFPSARERSSPRPLRTLAPELDSPRASMPVDYAAPAAPLGNPLENPNLSEKERVVAA
                     LEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF"
     gene            complement(6328..7848)
                     /gene="nifL"
                     /locus_tag="PKX01_11840"
     CDS             complement(6328..7848)
                     /gene="nifL"
                     /locus_tag="PKX01_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HMZ76865.1"
                     /translation="MPALTRDKPQAILDALPSEAYRQAVDQADLAISITDPKARILFA
                     NEAFVRVTGYTPDEIIGQNESVLSNHTTPSSVYKEMWTELSAQRPWSGRLLNRRKDGA
                     LYLAELTISPVVDERGATTHFLGMHRDVTELHRLEKVVRNQKKLIESVVDSAPTAFAL
                     LDQSGRLILDNQEYKKLVADLRVKEPAHTLLDSVLANWREELAARPERCIFFNREARV
                     DRPGGRPRWLSVSATTLATQSDLADAYFSVGGQTGLLLVISDVSSVREEQERARTASL
                     QAVLAEEERTASLREGLSAAIFRLEEPMNVMASAVSMLQRRDPSAAAMLKQALTASRE
                     HMENLRQVIPHSPPEMVIPVNVNEILRDVLDICTPRLLSAGVVVDWSPAATLPPVIGR
                     PQQLRLLFKALMENAIEAMNVKGWKRRELSLSTAVERDCIVVSVIDTGPGIPAELRHK
                     AFEPFFTNKSSGRHLGTGLSRAQQIVSDHGGIIDLSPSPSGGCAAVVEFRLDGDPI"
     gene            8151..8732
                     /gene="rsxA"
                     /locus_tag="PKX01_11845"
     CDS             8151..8732
                     /gene="rsxA"
                     /locus_tag="PKX01_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HMZ76866.1"
                     /translation="MSELLLLLLTTALINNVVLVKFLGLCPFMGVTKKLDTAFGLGLA
                     TTFVITMASGACWMLENWILIPFDISYLRILMYILVIAAVVQFVEMVVKKMAPELYQA
                     LGVYLPLITTNCAVLGLPLISAQEKHTLFHAIMFGFGSSVGFTLVLAMFAGMRERLAL
                     ARIPGAFSGAPIGFVTASLLALAFMGFSGLNFN"
     gene            8755..9285
                     /locus_tag="PKX01_11850"
     CDS             8755..9285
                     /locus_tag="PKX01_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HMZ76867.1"
                     /translation="MIIAIASLTLLGLFLGLGLGVADRIFKVESNPFIAEIEAMMPGS
                     NCGQCGFPGCSGAAKALVEGHAPVTCCPPGGKALATALAARLGVSVDLSQVKDDGPRI
                     ALVSEELCIGCCRCSKVCPTDAIIGAAKQIHNVLREACTGCSSCVEKCPTEALSMKPV
                     PVTLQHWVWPKPAAAA"
     gene            9299..10789
                     /gene="rsxC"
                     /locus_tag="PKX01_11855"
     CDS             9299..10789
                     /gene="rsxC"
                     /locus_tag="PKX01_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HMZ76868.1"
                     /translation="MGFFDKIFSRHWGVHPEDHKRPAADAPVLKLPVAPRLFIPLSQH
                     VGAPARPQVVVGQRVLKGQCLAEAQGNISAPIHAPTSGTIVSIGEITAPHPSGLPLVA
                     ISIEPDGEDRWIDCKGLTDPFSESPQALAKRVAEAGVVGLGGATFPASVKLALGLRSK
                     IDTLIINGSECEPYLSCDDRLMRDKAADILSGVRVMLHSTGAQQALVGIEDNKPEAIA
                     AMKEAAAKYGEIRICPVPARYPMGSDRQLIVELTGREVPSDARAADVGVLVHNVGTAN
                     AVHQAIYEGRPLVSRLITLNGGAMAGPGNYEVLIGTLISDVIAFAGGYKGTPARLIMG
                     GPMMGTVLPHAAVPVVKGTAGVLAFTAAEVEEREARNCIRCGSCIKACPMGLMPLEMA
                     NFIRAEKLDGAEKIGLSDCISCGCCSYVCPSNIPLVQYFTYAKGAISAQTRAQLRQDA
                     TKKLAQARQERLDREAREKAEAAAKRKAERAAAQAAATKHTEGANA"
     gene            10786..11871
                     /locus_tag="PKX01_11860"
     CDS             10786..11871
                     /locus_tag="PKX01_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HMZ76869.1"
                     /translation="MTQTTATVAAPVVAPHVAGSAGVARTMFLVVGALTPATLYGFWL
                     YGWPAIILFMLTLGSCVFFEYLCLHLAGAAPKRHVTDGSAILTGWLLAMTLPPWAPWW
                     TAVVGGFIAIVFGKQVFGGLGQNPFNPAMVARVALLVSFPVPLTLWVSPMPITAADAP
                     GFIDALRIVFGTLPIPDGVTSASLLGHVKTEMSRGVDLFHSLNGEHGHLLSYAGERAG
                     SLGETATWLILAGGAALLYLRVITWHIPAAMLLGVVVPAAIGHAVNPARFLDVTTHLM
                     SGGVMLGAFFIATDYVTSPSTTTGKLIFGFGCGFFTFVIRTWGGYPEGVAFSVLLMNT
                     LTPVIDRFTAPRILGRDRRGRPLQIKS"
     gene            11885..12523
                     /gene="rsxG"
                     /locus_tag="PKX01_11865"
     CDS             11885..12523
                     /gene="rsxG"
                     /locus_tag="PKX01_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HMZ76870.1"
                     /translation="MDIKALQEKLGYHGVFLGLVGLLASAALATADMATHEDIRLAER
                     RDLEASLKQVLPQGFADNDLLKDTVTLARGDGKEVKVFRARLGGEAKGAVFEVSSRGY
                     AGDVVALLGVDTAGVVQGVRVLKHQETPGLGDKIEVAKSKWILDFDGKSFGTPPVEKW
                     GVKKDGGVFDQFAGATITPRAVVKAVREGMEFYRDHKDEIYSKPADGGGKEH"
     gene            12525..13217
                     /locus_tag="PKX01_11870"
     CDS             12525..13217
                     /locus_tag="PKX01_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HMZ76871.1"
                     /translation="MSEYRDICTDGLWKQNIVFSQILGMCPTMAVTTSGTNGLGMGLA
                     TTAVLLVSNALVSSVRNVTTPQVRIPVYIVLIACLVTLVDLGINAWMHDLYKVLGMYI
                     ALIVANCAVLGRAEAFAARKPVLQSAMDGLWMGLGFTFALTTIGLCREFVGQGTLFSQ
                     ASLLLGPSFSFLEINALPGYGGTLLMILPPGGFLTLGLLLAAKQAWERHQAARASAAP
                     PLPMAPAAHCGG"
     gene            13231..13500
                     /locus_tag="PKX01_11875"
     CDS             13231..13500
                     /locus_tag="PKX01_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HMZ76872.1"
                     /translation="MQIGVAYSEPGQQIWLNIEVPDESTVEDAIQRSGILKQFPHIDL
                     AAQKVGVFGRLVKLNAALKPGDRVEIYRGIIADPETVPRRDQDEE"
     gene            13738..15138
                     /gene="nifE"
                     /locus_tag="PKX01_11880"
     CDS             13738..15138
                     /gene="nifE"
                     /locus_tag="PKX01_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HMZ76873.1"
                     /translation="MKASEIQALLDEPACTHNKKEKSGCARPKPGATAGGCSFDGAQI
                     ALLPIADVAHIVHGPIACAGSSWDNRGTRSSGATLYRIGMTTDLSETDVVMGRGEKRL
                     FHAIKQAVETYSPAAVFVYNTCVTALIGDDVEAVCKAATERWGTPVVPVDAAGFYGTK
                     NLGNRLAGEAMFKHVIGTREPEPAAPRVDGRPTYDVNLIGEYNIAGEFWHVSPLFDEL
                     GLRILCTLSGDARFHEVQTMHRAKVNMVVCAKALLNVARKMQDTYGIPFFEGSFYGVQ
                     DMSTALRDFARIIGDPDLIARTEAVIAREEAKSRADLDPWRERLTGKRVLLYTGGVKS
                     WSIVSALQDLGMKVVATGTKKSTEEDKARIRELMGDDTKMIDDGSPKALLSIYKEYKA
                     DILIAGGRNLYTALKARIPFLDINQEREFGYAGYDGMVELARQLALSMNSPVWEAVRR
                     PAPWAKQSGPGTVLAA"
     gene            15149..>15941
                     /gene="nifN"
                     /locus_tag="PKX01_11885"
     CDS             15149..>15941
                     /gene="nifN"
                     /locus_tag="PKX01_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HMZ76874.1"
                     /translation="MAEIVHSKKALAVNPLKVSQPVGASLAFLGIARSLPLMHGSQGC
                     TAFGKVFFVRHFREPIPLQTTAMDQVSTIMGADDNVVEALRTLSDKSKPDLIGLVTTG
                     LSETQGTDIRRAVRNFREAHPEFAHVAVVPVSTPDYVGCLESGYALAIESLIETLVPE
                     SSCAGRRPRQVNVLASGMLTPGDIEAIKEWVEAFGLRPVVVPDIGDSLDGHLVEAEYS
                     SLTLGGTPRQEIESMGESVATLVIGPSLYRAADKLKARTGVPDFRF"
BASE COUNT         2434 a         5285 c         5562 g         2660 t
ORIGIN      
        1 ccaaggcggt ggcggtgtgc atgaacaacc accaatgcca gcgcgacggc aagggcgtga
       61 tgtgccccag ctaccgcatc acccacgacc cggcccactc caccggcgcc cgcgtcaagg
      121 ccttcaaggc cgccctcaac ggcgagctgg gcggggcgcc gggcgacaat cccttcgccc
      181 acccggacct ggccgccgcg atggacctgt gcgtgtcctg caagggctgc aagaaggaat
      241 gccccagcgc ggtggacatg accctcatca agaccgaata cctggcccag cgcaacgccg
      301 tgcagggcgt gtcgcggcgg gcccgcctgt tcggtgcgct gccccgctgg ctgcaccgtt
      361 accgtggcgt gctggccgcc ctggtgcgcc tgcgcaacgc ctcaccgtgg gtggcgaaga
      421 aggccgaagg ctggctcggc atcgccgcgc agcgctccat cccgtccatc accggccacg
      481 gtgtggccga ccgccccggg ccggtcgacg ccccccgcgg ccgcgtggcg ctcttcgtcg
      541 ataccttcac ccactactac acgcccgaag tggccgaggc ggcccgcgaa gtgctggagg
      601 ccggcggcta cgccgtccag gtgctgcgcc ccgccgccgg cgacgacgaa ccggagcgcc
      661 ccctgtgctg cgggcgcagc tacctgtctc aggggctggt ggatgaggcc cgcgaggagg
      721 gccgccgcac gatggctgcc ctggtgcccc tgctcgccga gggcgtcccg gtggtcggcc
      781 tggagccctc ctgcctgctt gccctgcggg acgagttcta cagtctgtcc ctcggcccgg
      841 aggtcggcca gctgggcaag ctcgccttcc tgttcgagga attcctcatg cgcgaaggca
      901 acaagggcct caagctgccc ctcaagcccc ttccgggcgg ccgcgccgtg gtgcatggcc
      961 actgccacca gaaggccttc ggtgccatga aggccacccg caaggtgctg ggctggattc
     1021 ccggcttcga gttcgacatc gtcgacgcca gctgctgcgg catgtccggc agcttcggcc
     1081 tggaggccga gcactatgcg gcctcgctga agatgggtga actgagcctg ctccccgcgg
     1141 tccgccaggc agcccccgac gccccggtca tcgccgacgg cttctcgtgc cggcatcaaa
     1201 tcctcgacgg caccggacgc gaggcggagc acgtggcggt ggtgctgcgg cgggcgctgg
     1261 atgtgtgagg gcgggcggcg aggcattgct tgccggacca tggggtaggt tgtacagtaa
     1321 cgcgtacata tacagaggag ccccccatgg atgcgatcac ctacagcacg gcccgcgcga
     1381 atctggccaa gaccatggat cgcgtgtgcg aggatcacgc gccgctgatc atcacccgca
     1441 atggcgagca atccgtggtg atgctgtccc tcgaagacta caacgccctc gaagagacgg
     1501 cctacctcct acgcagcccc gccaacgcca ggcgcctgct ctccgccgtc gagcagttgg
     1561 ccgcgggaca aggcgttgag cgggatgtgt cactgtgagg ttgattttct cggatgcagc
     1621 gtgggaagac tatctgtact ggcagcatca ggaccgcaag atggtcgagc ggatcaacac
     1681 gctgattcaa gaagtcaaac gggagccgtt ttccggccgg ggaaaacccg aacccctgaa
     1741 gcacgctttg tccggattct ggtcccgcag gatcacggac gaacatcgga tggtgtaccg
     1801 catcgagggc gatgccgtgc tgctggcgca gttgcggtat cactattgaa gccgtggggg
     1861 caagacggct cacccttcgg gatgagcggc gcgctgcaac acctccaata cccattgatt
     1921 gagacgcttg cctgcagcct cggccacgac gagggcgctt gcagttttca gggctttccc
     1981 tgggcaacag gtacgatttc tggactttga gtactggcgt tgtcaatacc gcgaaaacag
     2041 atcccggatc ttgcacagcg tcgtggtgac gtcgaagcgc gggtccggca gggcttcgcc
     2101 ggcgaggatg tgggtgatgg cgagggcggg gtcgctctcg cgggtgagga ggacgtcggc
     2161 gcggcggtgg cgcatgtggc ggacgaagcc gtcgccggcg ctggcggcga ccaccacgtc
     2221 cacgccgtcc aggggatggg ggcggtcgtc cttgaagacg tgcatcactt cgtcctcggc
     2281 cagctcgatg cgttgggggg cgggcaggag ccggctggag tggtgttcac ccaggtcgta
     2341 caccagccac tggcgcgact ggccggcgtg gccactgaca atggtgaagt tctgggtggc
     2401 gatggcgact ttcatgatcg gggctcgtcg gcaggggtga gggtgaccgc cacgtccttg
     2461 ggcggggcga aatagggggc gctggtgacc aggatgtggg caccggcgcg cacgtaggct
     2521 tcggcgttgg cgggcgtgat gccgccggcc gcggcgaggc gcgggtggcc ggggccggcg
     2581 gagatctggg cgtgcagggc ggcgacggtg tcgggggaca ttttctcgag ctgcaggatg
     2641 tcgacgccgg cagcggccca ggcgagggcg tccgcggcct gggcgacctc gaccacgacc
     2701 gggcgctcgg gccactggcg gcgcaggtgg gcgagggtgg cggcgggcga ctcgtccggc
     2761 ggcagaaagg cgcggtgctc ggggaagatc agcagggtct cggagaggct gatgcggtgc
     2821 gggctggcgc cggcgcacag gatggccttg agggcggcgc gcttggtgcc ggggaagttc
     2881 ttgcgggtgc acaccacgcc cgcgtccggg tgggcgcggc gtgcagcggc gaggagttcg
     2941 gcggcgcagg tggcgatgcc cgagaggtat tccaccaggg tctgggcggt cttccacacg
     3001 gcgtgaagcg cgtcggcggg gccgctgagg ctgaggaagg ccgtgccggc gtcgaggtgg
     3061 ctgccgctgg gcaggaccgg cccgtcgacg ttgaggccgc ggagcacgcc catgcgggcg
     3121 gcttcctcgc tgccgcacag ggtcatggcg tagcgggcgc gcaggaccat gcgcccccgt
     3181 cggctgccga tgcccagcgt gtgggtggtg gcgtcgccgc agggggtgtc gtcctggagc
     3241 agggcttcca ggtcggtgtc gggcagggtg taggggttca tcggcctccg tggacgtgaa
     3301 acccggtcag gcgctcaccg gggcggccat ctcggcgcgg atctgggcga gccaggcgtc
     3361 gaggcgggcc tcggtcagga caggctggtg gtgctggtcg agggcgaggc cgaggaagtg
     3421 ctcgccatcc tgggccttgg actggttgaa ttcataaccg gccacgggcc agcgccccac
     3481 gatgcgcact ccgcgggcga gcagggcgtc gtggagcagg ctgatggcgt ccacgaactc
     3541 gtccggatac ttcttctggt cgcccaggcc gaagatcgcg gcggtgcggc cgcgcagatc
     3601 cacgccgtcg agctggggca ggaactcttc ccagctgggc tggctgaggc ccacggactg
     3661 gccgggcagt tcgccgtcgc ccagggtggg cgagccgagg atccagcagt cgtaggcgag
     3721 gaagtcgtca agcgtggcgc gcccgatgtt gaccggcgcg gctgccgcgt cgccgagctt
     3781 tttcgcgatc tgcttggcga tgaggcgggt ccggccggtg tcggtgccga agaagatgcc
     3841 gatgggcatg gtgtcagttc cggacgggga catcctgggt gagttcgtcg ggcaggcagc
     3901 ccaccggggc gccgagggtg ccgtcggggc cgttctcgaa gcgcacgagg tagatctcct
     3961 tgctctcgtc gagttcggcg tagccgaaat ggaccaccac gccgcgggtg ccctcggcga
     4021 cgatgaggcc ttcctcctcg gtgacgccgg gcatgccgcc gtcgttgagg aggtcttctg
     4081 cggcgaagac catatcgccg atctcgtact ggacggtgct cacggtctca ctcccacacg
     4141 tcggcggggg ccttgaggcg ctccaccggc tgatcgaaca tcaggtgctc gtcgatgatg
     4201 gtggccacgt cctccggttt gatgcccacg tacatcgtgc cttccggata gacgatggcc
     4261 gtggggccca ggttgcaggg gcccaggcag ccggtgttgg tgagctggaa gcccgaagcc
     4321 cacaggttgc gggtggtgaa ttcatccgag aaggcctgcc acacgggggc gcagccgttg
     4381 gcctgacagg acccgcgggg atggccgggc gggcggccct ggacgcagac gaggacgtgt
     4441 ttcttggggc ggggcatggt gtctctccgg gttgggtgag cacttccttg caagcccgat
     4501 gccagattaa atcacttgaa attcataggc ttggggcctg tgtgggatgt cgggtttgcg
     4561 acaatccggg gggcggcccg gtcagaactg cttgacctcg atattgagcg tctggatgcg
     4621 gtaggcgatc tggcgcgggg tcatgcccag aatgcgggcg gccttggcct gcacccagcc
     4681 ggcttgttcg agggccgcca cgacgcgctc cttctcggag agattgggat tctcgagggg
     4741 attgccgagc ggtgcggccg gcgcggcgta gtccaccggc atgctggcgc gcgggctgtc
     4801 cagttccggg gcgagggtgc gcagggggcg cggcgagctg cgctcgcggg cgctgggaaa
     4861 gcggatcagc tcggcgtcga tgcggccatc ctcggagagc acggcggccc gctccagaca
     4921 gttttccagc tcgcgcacgt tgcccggcca gtcgtagctg gcgaggcggc ggttggccat
     4981 gtcggtgagg gccagcttgc gcttctgctc gccgccgatc ttgttgagga ggtggcgggc
     5041 gatctccagg atgtcttcga cacgctcccg cagcgggggc aggaagaggg gcatcacgtt
     5101 gaggcggtag aacagatcct cgcggaagtc gcccatctcc acggcggttt ccaggtcccg
     5161 gtgggtggcc gcgatgatgc gcacatccac cttgatggtg cggctgccgc cgacccgctc
     5221 gaactcgcct tcctggagca cacgcagcat cttggcctgg aaggtggcgg agatctcgcc
     5281 gatttcgtcg aggaacaggg tgccgccgtg ggcctgctcg aagcggccct tgcgcgcctc
     5341 gacggcgccg gtgaaggcac cccgctcgtg gccgaagagt tcggattcga ggaggttttc
     5401 gggcagggcc gcacagttga gacgcacgaa ggcgttgcgg gcgcggggcg agttgtagtg
     5461 gatggcgttg gcgatgagct ccttgccggt gccggtctcg ccgcggatca gcacggtggt
     5521 gttccatttg gccaccatgc gggcctggtc gaagacccgg cgcatcaccg ccgagcgccc
     5581 caccatgctg tcaaagccgt actggtggcg cacggtgcgg cgcagctggt cgcgctcttc
     5641 gacgagggag gatttctcct gctcgacttc gagggacagg cgcaggttct ggccgatgag
     5701 gttggccacc atctccacga actgggcgcg ctcctccagc aggccgtcgt ccgggcgttc
     5761 cggttgcacc gccaggacgc cctgcagggt gctgccggcc ttgatgggca cggcgatgaa
     5821 gggcaggccg ggctggtaga tgccgaggcg gttgaggaag cgcggttcgt cggcgatgcg
     5881 ttgcagcatg atggtgcgcg gcttctcgag gatggtcccg agtacgccct cgccgggctg
     5941 gtaggagacg tcgccgacga cgttggggtg atggatcacg tgggtgccga gggtgccgga
     6001 ctgggcatcc actacggcga tgaggcccag gccgaggccc gcctcctcgt cgaggacgcg
     6061 caggacgtcg cgcagggtct cgccgaaggc cagggagcgg ctgaggaccc ggctgacggc
     6121 gtagagggcc gccaggagct ggatgttcag ctcgtgggtg cgacagaaac cgcccacagg
     6181 ggggcattcg tcgaggagtt tcgggcgttc ttgcatgccg tgggcgctca caggttatgt
     6241 ccttcagggc cgccgcgcct gggggcggtg gcggacgaaa gtcttctagc cggcgccgcg
     6301 ggcggcgccc ggtgcgggac ggcgggctta gatcgggtcg ccgtccaggc ggaattccac
     6361 gacggccgcg cagccgccgc tggggctggg gctcaggtcg atgatcccgc cgtggtccga
     6421 cacgatctgc tgggcgcggg acaggccggt gcccaggtgg cggccgctgc tcttgttggt
     6481 gaagaagggt tcgaaggcct tgtggcgcag ctcggccggg atgccggggc cggtgtcgat
     6541 gaccgagacg acgatgcagt cccgctccac ggcggtggac aggctcagct cgcggcgctt
     6601 ccagcccttc acgttcatcg cctcgatggc attctccatc agggccttga agagcaggcg
     6661 cagttgctgc ggccgcccga tgaccggggg cagggtggcc gccggtgacc agtccaccac
     6721 cacgccggcc gagagcaggc gcggcgtgca gatgtccagc acgtcccgca ggatctcatt
     6781 gacgttgacc gggatgacca tctcaggcgg gctgtggggg atcacctggc gcaggttctc
     6841 catgtgctcg cggctggcgg tgagggcctg tttgagcatg gcggcggcgg acggatcgcg
     6901 ccgttgcagc atggagacgg ccgaggccat cacgttcatg ggttcttcga ggcggaagat
     6961 ggcggcggag aggccttccc gcagggacgc ggtgcgctct tcctcggcca gcacggcctg
     7021 cagggaggcg gtgcgggcgc gttcctgttc ctcgcgcacg ctgctcacgt cggagatgac
     7081 cagcagcagg ccggtctgac cgccgacgct gaaataggcg tccgccagat cgctctgggt
     7141 ggcgagggtg gtcgcgctca ccgacagcca gcgcggccgg ccgccgggcc ggtcgacgcg
     7201 ggcttcccgg ttgaagaaga tgcagcgctc ggggcgggcg gcgagttcct cgcgccagtt
     7261 ggcgagcacg ctgtcgagca gggtgtgggc gggctccttg acgcgcaggt cggcgaccag
     7321 cttcttgtat tcctgattgt cgaggatcag gcggccgctc tggtccagca gggcgaaggc
     7381 ggtgggcgcg gaatccacca ccgattcgat gagcttcttc tggttgcgca cgaccttttc
     7441 gaggcgatgc agctcggtca cgtcccggtg catgccgagg aagtgggtcg tggcgccccg
     7501 ttcgtccacc actggcgaga tggtcagctc tgccagatac agggcgccgt ccttgcgccg
     7561 gttgaggagc cggccggacc aggggcgctg ggcggacagc tcggtccaca tctccttgta
     7621 aaccgacgac ggggtggtgt ggttggacag caccgattcg ttctggccga tgatctcgtc
     7681 cggcgtgtag ccggtgaccc gcacgaaggc ctcgttggcg aagaggatcc tggccttggg
     7741 gtccgtgatg gagatggcca gatccgcctg gtccaccgcc tggcgatagg cttcggaggg
     7801 cagcgcatcg aggatcgcct ggggtttgtc gcgggtgagg gccggcatgg ggggctcctg
     7861 agacggaatc gcggtgacgg acgggggcgt gtaacgccgg aaatttcaag catgttccat
     7921 gccgaaagcc ctaaatccct gtctggccgt ggttttgttg cggcgcggtg gtttctgggg
     7981 caagtgcgac gaaagtgctg tcggttttgc gacactgatt cgggcgcgtc gcgcaccggc
     8041 ttggtgcctg cgaacgaaaa cccccggaat tgaagggttt ttgcagcgcg gcatcggact
     8101 ggcacggctt gtgccttgag ggcatcaatc ccctcattcg agatgccgcc atgtccgaat
     8161 tgctcctgct cctgctgacg accgcgctga tcaacaacgt ggtgctcgtc aaattcctcg
     8221 gcctgtgtcc cttcatgggc gtgaccaaaa agctcgacac cgccttcggc ctcgggctcg
     8281 ccaccacctt cgtgatcacc atggccagcg gcgcctgctg gatgctcgag aactggatcc
     8341 tgatcccctt cgacatctcc tacctgcgca tcctgatgta catcctggtg atcgccgcgg
     8401 tggtgcagtt cgtcgagatg gtggtgaaga agatggcgcc cgagctctac caggccctcg
     8461 gcgtgtatct gcccctgatc accaccaact gcgcggtgct cggcctgccc ctcatcagcg
     8521 cccaggaaaa gcacacgctg ttccacgcca tcatgttcgg cttcggctcc tcggtgggtt
     8581 tcaccctggt gctggccatg ttcgccggca tgcgcgaacg cctcgccctg gcccgcatcc
     8641 ccggcgcctt ctcgggcgcg cccatcggtt ttgtcaccgc gagcctgctg gccctggcct
     8701 tcatgggctt ctcgggcctg aacttcaact aaaacccgcg acaggagaac cccaatgatc
     8761 attgcgattg cgagcctgac cctcctcggc ctgttcctgg gcctggggct gggggtcgcc
     8821 gaccggatct tcaaggtgga gagcaacccc ttcatcgccg agatcgaggc gatgatgccg
     8881 ggttccaact gcggccagtg cggctttccc ggctgctccg gtgccgccaa ggccctcgtc
     8941 gaaggtcacg cccccgtcac ctgctgcccg ccgggcggca aggccctcgc caccgccctc
     9001 gcggcgcgtc tgggcgtgtc cgtcgatctg tcccaggtga aggacgacgg tccgcgcatc
     9061 gccctggtgt ccgaggagct gtgcatcggc tgctgccgct gcagcaaggt gtgcccgacc
     9121 gacgccatca tcggcgccgc caaacagatc cacaacgtcc tgcgggaagc ctgtaccggc
     9181 tgcagcagct gcgtcgagaa gtgcccgacc gaagcgctga gcatgaagcc cgtccccgtc
     9241 accctgcagc actgggtctg gcccaagccg gccgccgctg cctgaggagc catcgatcat
     9301 gggttttttt gacaagatct tctcgcgcca ttggggcgtc catccggaag accacaagcg
     9361 tcccgccgcc gatgccccgg tgctgaagct gcccgtcgcg ccgcgcctgt tcattcccct
     9421 gtcccagcac gtgggcgccc cggcgcgtcc gcaggtggtg gtgggccagc gggtgctcaa
     9481 gggccagtgc ctggccgagg cgcaaggcaa catctcggcg ccgatccacg cacccacctc
     9541 gggcaccatc gtctccatcg gcgagatcac cgcgccgcat ccgtccggtc tgcccctggt
     9601 ggcgatctcc atcgagccgg acggcgagga ccgctggatc gactgcaagg gcctcaccga
     9661 ccccttctcg gagtcgcccc aggccctcgc caagcgcgtg gcggaagccg gcgtggtggg
     9721 cctcggcggc gccaccttcc cggcctcggt caagctggct ctgggcctgc gctccaagat
     9781 cgacaccctg atcatcaacg gcagcgagtg cgagccctat ctctcctgcg atgaccgcct
     9841 gatgcgcgac aaggccgccg acatcctcag cggcgtgcgc gtgatgctcc actccaccgg
     9901 cgcccagcag gccctggtgg gcatcgagga caacaagccc gaagccatcg cggccatgaa
     9961 ggaagcggcc gccaagtacg gcgagatccg catctgcccg gtgccggcgc gctacccgat
    10021 gggttccgac cgccaactga tcgtggaatt gaccggccgc gaggtgccct ccgacgcccg
    10081 cgccgccgac gtgggcgtgc tggtgcacaa cgtgggcacc gccaacgccg tgcatcaggc
    10141 gatctacgaa ggccgcccgc tggtgtcgcg cctgatcacc ctgaacggcg gcgccatggc
    10201 cggccccggc aactacgagg tgctgatcgg caccctgatc tccgacgtga tcgccttcgc
    10261 cggcggttac aagggcaccc cggcacgcct gatcatgggc ggcccgatga tgggcaccgt
    10321 gctgccccac gcggcggtgc cggtggtcaa gggcaccgcg ggcgtgctgg ccttcacggc
    10381 ggccgaggtg gaagagcgcg aagcgcgcaa ttgcatccgc tgcggctcct gcatcaaggc
    10441 ctgcccgatg ggcctgatgc ccctggagat ggccaacttc atccgcgccg aaaagctcga
    10501 cggggccgag aagatcggcc tgtccgactg catctcctgc ggctgctgct cttacgtgtg
    10561 tccgtccaac atcccgctgg tccagtactt cacctacgcc aagggcgcca tctccgccca
    10621 gacccgcgcc cagctgcgcc aggacgccac caagaagctg gcccaggccc gtcaggagcg
    10681 tctggatcgg gaagcccgtg aaaaggccga agccgccgcc aagcgcaagg ccgaacgggc
    10741 tgcggcccag gccgccgcca ccaagcacac cgagggagcc aacgcatgac ccagaccaca
    10801 gccactgtgg cggcgcccgt cgtcgccccc cacgtcgccg ggagcgcggg tgtcgcccgc
    10861 accatgttcc tggtggtcgg cgccctgacg ccggcgaccc tgtacggctt ctggctgtac
    10921 ggctggccgg cgatcatcct gttcatgctg accctcggct cctgcgtgtt cttcgagtac
    10981 ctgtgcctgc atctggccgg cgcggcgccg aagcgccacg tcaccgacgg ctcggccatc
    11041 ctcacgggct ggctgctggc catgaccctg ccgccctggg cgccctggtg gaccgcggtg
    11101 gtgggcggct tcatcgccat cgtcttcggc aagcaggtgt ttggcggcct cggccagaac
    11161 cccttcaatc cggcgatggt cgcccgggtg gccttgctgg tgtccttccc ggtgcccctc
    11221 accctgtggg tgtcgcccat gccgatcacg gcggctgacg caccgggctt catcgacgcc
    11281 ctgcgcatcg ttttcggcac cctgccgatc cccgacggtg tgaccagcgc ctccctgctg
    11341 ggccacgtga agaccgagat gtcccgcggc gtcgatctgt tccactccct taacggtgaa
    11401 catggccacc tgctgtccta cgccggcgag cgcgcgggca gcctgggcga aaccgccacc
    11461 tggctgatcc tggcgggcgg tgcggcgctg ctctacctgc gggtgatcac ctggcacatc
    11521 ccggcggcca tgctcctcgg cgtggtggtg ccggcggcca tcggccacgc ggtcaatccg
    11581 gcgcgtttcc tggacgtgac cacccacctg atgtccggcg gcgtgatgct cggcgccttc
    11641 ttcatcgcca ccgactacgt gacctcgcct tccacgacga cgggcaagct gatcttcggt
    11701 ttcggctgtg gcttcttcac cttcgtcatc cgcacctggg gcggctaccc ggaaggggtg
    11761 gccttctcgg tgctgctgat gaacaccctg acgccggtca tcgaccgttt caccgcgccc
    11821 cgcatcctgg gacgcgaccg ccgcggccgc ccgctgcaga tcaagtcctg acccggagaa
    11881 cagcatggat atcaaggccc tacaggagaa gctcggctat cacggggtgt tcctcggtct
    11941 cgtcggcctg ctcgccagcg ccgccctcgc caccgccgac atggccaccc acgaggacat
    12001 ccgtctggcg gagcggcgcg atctggaagc ctccctcaag caggtgctgc cgcaaggttt
    12061 tgccgacaac gacctgctca aggacaccgt gaccctcgcc cgcggcgatg gcaaggaggt
    12121 gaaggtcttc cgcgcccgtc tgggcggcga ggccaagggc gccgtcttcg aggtgagctc
    12181 ccgcggctac gccggtgacg tggtggcgct cctcggtgtg gatacggccg gcgtggtgca
    12241 gggcgtgcgc gtcctcaagc accaggagac gccgggcctg ggtgacaaga tcgaagtggc
    12301 caagagcaag tggatcctgg acttcgatgg caagtccttc ggtacgccgc cggtcgagaa
    12361 gtggggcgtg aagaaggacg gcggcgtctt cgaccagttt gccggcgcga ccatcacgcc
    12421 gcgggcggtg gtcaaggcag tgcgggaggg catggagttc taccgcgacc acaaggacga
    12481 aatctattcc aaaccggccg acggcggcgg aaaggagcat tgacatgagc gagtaccgcg
    12541 acatctgcac cgatgggctg tggaagcaga acatcgtctt ctcccagatc ctgggcatgt
    12601 gccccaccat ggcggtgacc accagcggca ccaacggcct cggcatgggg ctggcgacca
    12661 ccgccgtgct gctggtctcc aacgcgctgg tgtccagcgt gcgcaacgtg accacgccgc
    12721 aggtgcggat cccggtgtac atcgtgctga tcgcctgcct ggtgaccctg gtggacctgg
    12781 gcatcaacgc ctggatgcac gacctctaca aggtgctggg catgtacatc gcgctgatcg
    12841 tggccaactg cgccgtgctc ggccgcgccg aagcctttgc cgcgcgcaag ccggtgctcc
    12901 agtcggccat ggacggcctg tggatgggcc tgggcttcac cttcgccctc accaccatcg
    12961 gcctgtgccg ggaattcgtc ggccagggca ccctgttctc gcaagccagc ctgctcctcg
    13021 gcccgtcctt ctccttcctg gagatcaatg ccctgccggg ctacggcggc acgctgctga
    13081 tgatcctgcc ccccggcggc ttcctcaccc tggggctgct cctggccgcc aagcaggcgt
    13141 gggaacgtca ccaggccgcc cgtgcctcgg ccgcgccgcc cctgcccatg gccccggcgg
    13201 cccactgcgg cggttgagga ggagacgacc atgcagatcg gcgttgcgta ttccgaacct
    13261 ggccagcaga tctggctcaa catcgaggtg cccgacgaat ccacggtcga ggacgcgatc
    13321 cagcgctccg gcatcctcaa gcagttcccc cacatcgacc tggcggccca gaaggtgggc
    13381 gtgttcgggc ggctggtcaa actcaacgcc gccctcaagc ccggcgaccg ggtggagatc
    13441 taccgcggca tcatcgccga cccggaaacc gtaccgcggc gggatcagga cgaagaatga
    13501 ctgcaggttg gcacttgcac agaaccccgg ctccggccgg ggttttttta ttgccggctc
    13561 gggcgcgtcg ggcgtgagcg gcgacctggc ctgtcgcaga tccctccgcg gcgtgtcgta
    13621 caaggcgggt ttgtcgggtt tgcgacatcg ccgcgctgca aaaactgccg tgctttcagg
    13681 ggcttgtgtt tggcatccat cttgctgttc gtaggatgga catctggaga gaccagcatg
    13741 aaagccagcg aaatccaggc tctgctcgac gagccggcct gcacccacaa caagaaggaa
    13801 aagtccggct gtgcccggcc caagcccggc gccaccgcgg gcggctgctc cttcgacggg
    13861 gcgcagattg cgctcctgcc catcgccgac gttgcccaca tcgtgcatgg ccccatcgcc
    13921 tgcgcgggtt cgtcctggga caaccggggc acgcgctcgt cgggggcgac gctgtaccgc
    13981 atcggcatga ccaccgatct ctccgagacc gacgtggtga tggggcgcgg cgagaagcgg
    14041 ctgttccacg ccatcaagca ggccgtcgag acctactcgc cggctgcggt gttcgtctac
    14101 aacacctgcg tcaccgccct catcggcgac gacgtggagg ccgtgtgcaa ggcggcgacc
    14161 gagcggtggg ggaccccggt cgtgcccgtg gatgcggccg gtttctacgg caccaagaac
    14221 ctgggcaacc gccttgccgg cgaagccatg ttcaagcacg tgatcggcac ccgcgaaccg
    14281 gagcccgccg cgccccgggt ggacggccgt cccacctacg acgtgaacct gatcggcgaa
    14341 tacaacattg ccggcgagtt ctggcatgtc tcgcccctct ttgacgagct gggcctgcgc
    14401 atcctgtgca ccctgtcggg cgacgcccgc ttccacgagg tgcagaccat gcaccgcgcc
    14461 aaggtgaaca tggtggtgtg cgccaaggcg ctgctcaacg tggcccgcaa gatgcaggac
    14521 acctacggca ttcccttctt cgaaggcagc ttctacggcg tgcaggacat gtccaccgcg
    14581 ctgcgggact tcgcccgcat catcggcgac ccggacctga tcgcccgcac cgaggccgtc
    14641 atcgcccgcg aggaggccaa gtcccgcgcc gacctggatc cgtggcgcga acgcctcaca
    14701 ggcaagcgcg tgctgctcta caccggcggc gtcaagtcct ggtccatcgt ctcggccctg
    14761 caggatctgg gcatgaaggt ggtggccacc ggcaccaaga aatccaccga ggaagacaag
    14821 gcgcgcatcc gcgagctgat gggcgacgac accaagatga tcgacgacgg cagccccaag
    14881 gcgctgctgt ccatctacaa ggaatacaag gcggacatcc tcatcgccgg cggccgcaac
    14941 ctctacacgg ccctcaaggc gcggatcccc ttcctggaca tcaaccagga gcgggaattc
    15001 ggctacgccg gttacgacgg catggtggag ctggcccgcc agctcgccct gtccatgaac
    15061 agcccggtgt gggaggcggt gcgccgcccg gccccgtggg ccaagcagag cggtcccggc
    15121 accgtgctgg cggcctgagg acacgaccat ggcagagatc gtccattcca agaaggccct
    15181 cgcggtcaat cccctcaagg tcagccagcc tgtcggcgcg tccctggcct ttctggggat
    15241 cgcccgcagc ctgcccctga tgcatggttc ccagggctgc accgccttcg gcaaggtctt
    15301 cttcgtgcgg cacttccgcg agcccatccc gctgcagacc acggcgatgg accaggtctc
    15361 caccatcatg ggcgccgacg acaacgtggt cgaagccctg cgtaccctct ccgacaagag
    15421 caagccggac ctgatcggtc tggtcaccac cggcctctcg gagacccagg gcaccgacat
    15481 ccgccgcgcg gtgcgcaact ttcgcgaggc ccatccggag ttcgcccacg tggccgtggt
    15541 gcccgtgagc acgccggact acgtgggttg cctggaaagc ggctacgcgc tggccatcga
    15601 gtccctcatc gagaccctgg tgccggaaag cagctgtgcc ggacgccggc cgcgtcaggt
    15661 gaatgtgctc gcctcgggca tgctcacgcc gggcgacatc gaggccatca aggaatgggt
    15721 ggaagccttc ggcctgcgcc cggtggtggt gccggacatc ggcgactccc tcgacggtca
    15781 cctggtggag gcggagtact cgtccctcac cctcggtggc acgccgcggc aggagatcga
    15841 aagcatgggc gaatccgtcg ccacgctggt gatcggcccg tccctctacc gcgccgccga
    15901 caagctcaag gcccgcaccg gcgtgcccga cttccgcttt g
//
LOCUS       DAOQPA010000084        15856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1869_length_15856_cov_18.060946, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000084 DAOQPA010000000
VERSION     DAOQPA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_15856_cov_18.060946"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="rfaE1"
                     /locus_tag="PKX01_11890"
     CDS             complement(<1..1001)
                     /gene="rfaE1"
                     /locus_tag="PKX01_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HMZ76875.1"
                     /translation="MTPTLPDFSRGRVLIVGDVMLDRYWHGATSRISPEAPVPVVKVD
                     MDEFRAGGAGNVALNVATLGGGVSLVGLVGDDESAGLLRARLEAGRVACHFVPVAGSH
                     TITKLRVISRHQQLIRLDFEDGFTRAEAAAVTAAVRAGLDGVGAVVLSDYAKGTLADA
                     RALVHAARERGLPIVVDPKGTDFERYRGASVLTPNLSEFEAVVGRCADEASLRARGTA
                     LRDALDLDALLITRSERGMTLLQRGHPPLDLPTHAREVFDVTGAGDTVVALLAAGLAA
                     GLPLAEATALANVGAGVVVGKLGTATVSVGELAAALHAPAHAREVFDVTGAGDTVVAL
                     "
     gene            complement(1098..2858)
                     /gene="msbA"
                     /locus_tag="PKX01_11895"
     CDS             complement(1098..2858)
                     /gene="msbA"
                     /locus_tag="PKX01_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HMZ76876.1"
                     /translation="MKASLQLYRRLLGTIVPYWRVVAISIAAMVLAAGLEPVMPTLLK
                     SLVDKSLIQKDPIAAWQLPLFLMLTFLGKGIAEYVTNVASQWVANKAVADLRQQVFHH
                     QTALPLATHLAEPPGRMLSRLLYDIPQVGQALSTAWIIVIRDSLVIVGLTGYLIYTAW
                     ELTLLILAIAPVVAWIIRVASARLRTSNQDIQTITGRLTGMVDASLVGVKEIKIFGAR
                     DYEAARFAQVVDRLRKETMRAVRVSSVNVPLVQVLAAMAVSLVIYVASSLSANDRLSP
                     GEFVAFVTAMSMLFEPIRRLTNVNATIQSGLAGAQSIYELLDSPPEPEQPAGPTATLP
                     APPGGGSEVRFEHVSFTYPGQPAPAVADLSLIVPPGHTVALVGGSGSGKTTAVSLLAR
                     FFNPEAGHILLDGAPIDALPLSALRARIAMVGQQVVLFDDSVAANIAYGRPDIPRAAI
                     EHAARAAHAWEFIEKLPQGLDTPIGENGARLSGGQRQRIAIARAFLKDAPVLILDEAT
                     SALDTESERQVQAALGTLIKGRSTLVIAHRLSTIEGADTIVVMDKGRVVEQGRHAELL
                     ARDGAYARLYRMQFQDEAPA"
     gene            complement(2855..3517)
                     /locus_tag="PKX01_11900"
     CDS             complement(2855..3517)
                     /locus_tag="PKX01_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HMZ76877.1"
                     /translation="MAYDLTKPYGRFMAYMNMQLEDHGIVRSIYNNFYALPGGMYRCS
                     QPSPAQIRKYHRLYGIKTIINLRGENPHGSYDLEVDTCRELGIELINFNLYSRDMPSV
                     EEVLGARDLFERIAYPALMHCKSGADRAGLGATFYRHFRLGEPLDQIRELSPKYGHFT
                     IARTGILDFFLACYQADNARAPIDFLTWVQTRYDQKALTEKFDARSRLGTWFVDKVLR
                     RE"
     gene            3654..4295
                     /locus_tag="PKX01_11905"
     CDS             3654..4295
                     /locus_tag="PKX01_11905"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HMZ76878.1"
                     /translation="MPRAPGREEEMDEEGLVPFSGLIRYERHVPIHQVSYYLCGEIRE
                     PQYYTELFYTLRTASATDLVYLHINSPGGDFNTGLQIINNMLASEARVVTVLEARAYS
                     MAAFVFLSGDELIVHDNCQLMFHTYSGSFAGKGNEQLAEVQALGAWFEKVTTRICSPF
                     LSAADIDRILKGSDLWMDSDEIRHRLGRKGRPAGTRRGTRTAAKRLPDVPSAD"
     gene            complement(4333..4974)
                     /locus_tag="PKX01_11910"
     CDS             complement(4333..4974)
                     /locus_tag="PKX01_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HMZ76879.1"
                     /translation="MMFRASFQHALSAVLVTAALTGTATAATVTPSSYTYDQSTACGT
                     WCYHDPANTKLTDGVLGLAGWAVNAGNEWDGWVYQNRVNIDFDFGTSRHIDLVRVGTT
                     QDNLGDVVLPSIELYISNDKSTWTFVTGLTVPPDSAHNIDPFSTAPHDFLTLAGLGID
                     ARYVRVGAIANGPWIFVDEVQFQSRTTQPVPEPAALGLLAAGLASLAGTRRPR"
     gene            complement(5126..5554)
                     /locus_tag="PKX01_11915"
     CDS             complement(5126..5554)
                     /locus_tag="PKX01_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76880.1"
                     /translation="MNSIRSGPIRRPPKPITWWHALAGVLVGSLLATLLAWMLPRTDA
                     EELLLPVHATYAARPARGDGLPAGLSLRGIIPPSGDSPGIALLKEPGRPVVLVTEGHE
                     YAPDLRVVRVLQDRILIRQRGNGDDLILTLVDPSSGSPVP"
     gene            complement(5681..6313)
                     /locus_tag="PKX01_11920"
     CDS             complement(5681..6313)
                     /locus_tag="PKX01_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ76881.1"
                     /translation="MNRPFRPALLAAAIAAAVPAHAYVATAFDASVYTANTAALDSAL
                     GLTGIVAIEDFEDGVLIPGLTSNLTTYGASLPNHAWDGLTASTWGNALFTVSLTGIRQ
                     FGIGISDNDGGGQTVTVNGHFTLDLSTVAGYRGDTSGRATYVLIHAESGDADITRIAI
                     DNSLTPLFDHLVLSTDSGLAPSALPEPSSAALMLAGTALAGWLRGRNRMK"
     gene            complement(6474..7031)
                     /gene="cobC"
                     /locus_tag="PKX01_11925"
     CDS             complement(6474..7031)
                     /gene="cobC"
                     /locus_tag="PKX01_11925"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HMZ76882.1"
                     /translation="MELHLIRHPRPDVAPGVCYGQTDVGLAESAAAVAARLRPLLPAG
                     CALIASPLRRARLLAEALGTPQLDPRLMEIHFGDWEGQRYADLGSALDAWAAAPLDFA
                     PPGGETPRQMAARVLDFLHDLQATPPAAAVAIVAHGGPLRVIAGHLLGLPDTHWLGLD
                     FACGEATRIDCHDWGTVLRWFNRRA"
     gene            complement(7016..7786)
                     /locus_tag="PKX01_11930"
     CDS             complement(7016..7786)
                     /locus_tag="PKX01_11930"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HMZ76883.1"
                     /translation="MRTQLELFLTAVGFFTRLPVPGWVPFSVERLAHAARYLPAVGWL
                     VGGLGALAYGLASQVLPPDVAVVLSMVATLVATGAFHEDGLADTCDGLGGGLDRDRAL
                     AIMKDSRIGSYGAIGLVLALLLKALALVNLGVDADMPVILALLAAHPLSRLAPVTLMA
                     VLDYARTGHDAKAGAVARRLTPAELGIATLAGVLPLLLLDATEAGLALAATVIVTAWA
                     GRLFRRRLDGYTGDCLGATQQIAELAIYLALLAAWNFT"
     gene            complement(7792..8829)
                     /gene="cobT"
                     /locus_tag="PKX01_11935"
     CDS             complement(7792..8829)
                     /gene="cobT"
                     /locus_tag="PKX01_11935"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HMZ76884.1"
                     /translation="MHYTLIRPDRGLAPALQQRIDTKTKPLGALGRLEALALQIGLIQ
                     QTLEPELRQPHVMVFAGDHGAARAGVSAYPQDVTWQMVENFLAGGAAINVFARQAGMG
                     LSVVDAGVAHDFGPRPGLIAAKVAPGTANYLEAPAMDAAQRNAALAHGRELALGLAGT
                     GCNVVGFGEMGIGNTAAASLLTHCLTGADLATVIGRGTGLDDAGLARKRELLAHAVAR
                     GGRPADPLDALAQYGGFEIAMMAGAMLGAAEARMVLLIDGFIVTSALLVAHALAPAVL
                     DYCVFAHRSQEPGHAVQFDHLGAEPLVQLDLRLGEGTGAALAYPLVQAAVNFLNEMAS
                     FESAGVSGKAD"
     gene            complement(8904..9452)
                     /gene="cobU"
                     /locus_tag="PKX01_11940"
     CDS             complement(8904..9452)
                     /gene="cobU"
                     /locus_tag="PKX01_11940"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HMZ76885.1"
                     /translation="MSPELILGGARSGKSRHAEHLAAASGLPVTVIVTAEALDAEMAA
                     RIARHRTDRPAGWATVEAPRDLAGALVATAAHGRCTVVDCLTLWLANLLDGAEHLPPA
                     TPAEALPAFARERAAFLDALPTLPGRVILVANEVGLGLVPETPLGRLFRDEAGRLNQA
                     VAALCGRVTFVAAGLPLVLKGG"
     regulatory      9542..9783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9903..11759
                     /locus_tag="PKX01_11945"
     CDS             9903..11759
                     /locus_tag="PKX01_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76886.1"
                     /translation="MTIRPTLAALAVAAAFPTVFSSAAFAASNAPAAAAVSSAEQPVV
                     LVTATRQTQRADESLADVTVIERARIEEAGASTSLAELLARQPGIQMSSSGGPGKATD
                     VYIRGASPKHSLLIVDGVRLGSATLGASSWQDIPLTQIERIEIVRGPASALYGADAIG
                     GVIQIFTRRGEGAPKADAFVGVGSYGNKEVAAGVSGGTERLSYSLRGAYTDVAGYNAI
                     ASQAKQPYYYNADKDPYRNSSLSGNLAYTLMPGHELGATLLYATARNHYDSGAGYDDF
                     ADNVQSVWSAYSRNRFRAGWTSTLRIGQSVDDARTYASYAPTGATIKTTQDQVMWQND
                     ITLPLGTLLAALENLEQKAVNEGTFSVSRQIRSALLGWTASVGEHRMQFNARYDDNSQ
                     FGNKTTGYAGYGYQILPSLRVQGSVGSAFRAPTFNELYFPGFGNPNLKPENAYNKELG
                     LLWEQHGQSVSLTAFHNRITNLIAYDANFIPQNVNNARLQGVSLGYAGTLAGFDINAN
                     LDFLDPEDADTGKRLQRRADKTGNLYLSRRIAAFNVGAEWQGAAGRYDRTGEINRMGG
                     YGLVNAFVKYAVDRDWSLEARANNLLNKQYETAWGYNTAGANLFFGVRYAPR"
     gene            11823..12806
                     /locus_tag="PKX01_11950"
     CDS             11823..12806
                     /locus_tag="PKX01_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HMZ76887.1"
                     /translation="MGRVRQRAALVLLVLVAAALAAFAWALSAGELPVTAADLLAALG
                     GGAGSMEADIIRTLRLPRAVAAFACGGLLALSGALMQVLLRNPLADPYVLGVSGGAAV
                     GALGCLLAGVAGLWIDVGAFAGALVATLLVFGLARGDGSWTRTRLLLTGVIVAAGCGA
                     AVALMLSIAPEGRVKGMLFWLMGDASGVSNPWPALGVLAVGLLTALPFARELNLLARG
                     EVTARALGVRVGRLRALVYGLGALLTGVAVTTLGSVGFVGLIVPHLVRLVIGNDQRLL
                     LPAASLAGGVLLTLADTAARTVVAPVQLPVGVLTALIGVPVFLFLLARHPR"
     gene            12803..13585
                     /locus_tag="PKX01_11955"
     CDS             12803..13585
                     /locus_tag="PKX01_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76888.1"
                     /translation="MKMDVRPDVLAAEALAVQVGRRDVVRGLEVRVAPGDTLVILGRN
                     GAGKTTLLHTLAGLRPAAGGTVLLSGKPYADWPRGHEACLRGLLPQSQPDFFSATVLE
                     TALVGRHPHLGRWAWEGPDDEAIALAALATVGLAEYAARPVLTLSGGERQRLAIAALL
                     VQQPRLYLLDEPLAHLDLHHQIGVLDHFHRLAADGAGVVMVLHDINLAARYASHVLLL
                     DGAGGFACGPARDILTTARLSAAFGHPLRAVADGASTLFVPA"
     gene            13650..14282
                     /gene="cobO"
                     /locus_tag="PKX01_11960"
     CDS             13650..14282
                     /gene="cobO"
                     /locus_tag="PKX01_11960"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMZ76889.1"
                     /translation="MNEPTPPAPPADPDRDARHTARMARKKAVVDAAIAAATERRGVL
                     LVNTGNGKGKSSAAFGVLARALGHGLQCGVVQFVKGRSDTGEEAFFRRQPEVRWHVMG
                     DGFTWDTQDRAADMASARAGWEKARAFLRDPAIGLVVLDELNIVLKYAYLDVAEVVAD
                     LRARPPQQHVIVTGRGAPAALIDVADTVTEMGPVKHAFQAGVAAQAGVEF"
     gene            14484..15437
                     /locus_tag="PKX01_11965"
     CDS             14484..15437
                     /locus_tag="PKX01_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HMZ76890.1"
                     /translation="MHRIVVLEAGAVIATLRRPAFAHEWVEYAATRQEQVAERLAGAT
                     IAVVNKRQLGAELLAGLPTLRLIAIAATGYNNVDLEACRARGIVVCNVRGYARQTVPE
                     HVFALLLALARQIGPYHRSVAAGRWQRSEQFCYFDYPIRDLAGQTLGLLGAGSLGEGV
                     ARIAAAFGMHVLRGERKGAASVREGYVDFDTLIRTADVISLHCPLTEATRHLIGAAEL
                     AAMKPTALLLNTARGGLVDEAALAEALRARRIAGAGFDVLSEEPPRDNPLLAPDLLEA
                     GRFLLTPHVAWSSREAMQALADQLMDNIDAFAAGTPRNRLA"
     gene            complement(15451..>15856)
                     /locus_tag="PKX01_11970"
     CDS             complement(15451..>15856)
                     /locus_tag="PKX01_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HMZ76891.1"
                     /translation="GYPTIRGEVKYGEGSRIDLLLSGDGRPDCYVEVKNVTAAVSGGV
                     GYFPDAVTTRGAKHLREMAAMVAVGRRAVLVFCVQRADVAAVRPADHIDPAYGRALRE
                     ALAAGVEVLALGARVSPDEIVVDRALPVTVPA"
BASE COUNT         2351 a         5290 c         5937 g         2278 t
ORIGIN      
        1 agggccacca cggtgtcgcc agcgccggtg acgtcgaaga cttcgcgggc gtgggcgggc
       61 gcgtgcaggg cggcggccag ctcgcccacc gacacggtgg cagtgcccag cttgcccacc
      121 accacgccgg cacccacgtt ggccagggcg gtggcctcgg ccaggggcag gccggcggcg
      181 agcccggcgg cgagcagggc caccacggtg tcgccagcgc cggtgacgtc gaagacttcg
      241 cgggcgtggg tgggcaggtc gagggggggg tggccgcgct ggagcagggt catgccccgc
      301 tcgctgcggg tgatcagcag ggcgtccagg tcgagggcgt cgcgcagggc ggtgccgcgg
      361 gcgcgcaggc tggcctcatc ggcgcagcgg ccgaccacgg cctcgaattc ggacaggttg
      421 ggcgtgagca cgctggcgcc gcggtagcgc tcgaagtcgg tgcctttggg gtccaccacg
      481 atgggcaggc cgcgttcgcg ggcggcgtgg acgagggcac gggcgtcggc cagggtgccc
      541 ttggcgtaat cggacagcac caccgccccc acaccgtcga ggccggcgcg gacggcggcg
      601 gtgacggcgg cggcttcggc gcgggtgaag ccgtcctcga agtccaggcg gatgagctgc
      661 tggtggcggc tgatgacgcg cagcttggtg atggtgtggc tgccggccac cgggacgaag
      721 tggcaggcga cgcggccggc ctcgaggcgg gcgcgcagca gtccggcgga ttcgtcgtcg
      781 cccaccagcc ccaccaggct cacgcccccg cccagggtgg cgacgttgag ggccacgttg
      841 ccggcgccgc cggcgcggaa ttcgtccatg tccaccttga ccaccggcac cggggcctcc
      901 ggcgagatgc gcgaggtggc gccgtgccag tagcggtcga gcatcacgtc gccgacgatg
      961 agcacgcggc cgcgggagaa gtcgggcagg gtcggggtca tggcgagagg ggacgaatga
     1021 cggaaagagg cgccgggaag ggcggggtac cggttgaagc ctgcattatt tcatgaatcg
     1081 gctgggaccc cgccggctca ggcgggggct tcgtcctgga actgcatgcg gtagaggcgt
     1141 gcataggcgc cgtcgcgggc gagcagttcg gcatggcggc cctgttccac gacgcggccc
     1201 ttgtccatca cgacgatggt gtcggcgcct tcgatggtgg acaggcggtg ggcgatgacc
     1261 agggtgctgc ggcccttgat cagggtgccg agggcggcct ggacctggcg ttcggattcg
     1321 gtgtcgaggg ccgaggtggc ctcgtcgagg atcagcaccg gcgcgtcctt gaggaaggcg
     1381 cgggcgatgg cgatgcgctg gcgctggcca ccggagaggc gcgcgccgtt ctcgccgatg
     1441 ggggtgtcca ggccctgggg cagcttttcg atgaattccc aggcatgggc ggcgcgggcg
     1501 gcgtgctcga tggccgcgcg ggggatgtcc gggcgaccgt aggcgatgtt ggcggcgacg
     1561 ctgtcgtcga agagcacgac ctgctggccg accatggcga tgcgggcgcg cagggcgctg
     1621 aggggcaggg cgtcgatggg cgcgccgtcg agcaggatgt gcccggcttc cgggttgaag
     1681 aagcgcgcca gcaggctcac cgcggtggtc ttgccgctgc ccgagccgcc caccagggcg
     1741 acggtgtggc caggcggcac gatcaggctg aggtcggcca cggccggggc gggctggccg
     1801 ggataggtga agctcacgtg ctcgaagcgc acctcgctgc cgccgccggg gggcgcgggc
     1861 agggtcgccg tcgggccggc gggctgttcc ggttccggcg gggaatcgag gagctcgtag
     1921 atgctctggg cgccggcgag gccgctctgg atggtggcgt tgacgttggt caggcgccgg
     1981 atcggttcga agagcatgga catggcggtg acgaaggcga cgaactcgcc gggcgacagg
     2041 cggtcgttgg cggacaggct ggaggccacg tagatcacca gggacacggc catggcggcg
     2101 agcacctgta ccaggggcac gttgaccgag gagacgcgca cggcgcgcat ggtctccttg
     2161 cgcaggcggt ccactacctg ggcgaagcgg gccgcctcgt agtcccgcgc gccgaagatc
     2221 ttgatctcct tcacgcccac cagggaggcg tccaccatgc cggtgagccg gccggtgatg
     2281 gtctggatgt cctggttgga ggtgcgcagg cgggcgctgg cgacacggat gatccacgcc
     2341 accaccgggg cgatggcgag gatcaggagg gtcagctccc acgcggtgta gatgaggtag
     2401 ccggtcaggc cgacgatcac cagggagtcg cggatgacga tgatccacgc ggtggacagg
     2461 gcctggccca cctgggggat gtcgtagagc aggcgcgaca gcatgcgccc gggcggctcg
     2521 gccaggtggg tggcgagggg cagggcggtc tggtggtgga agacctgctg gcgcaggtcg
     2581 gccacggcct tgttggcgac ccactggctg gccacgttgg tgacgtactc ggcgatgccc
     2641 ttgccgagga aggtgagcat caggaacagg ggcagctgcc acgcggcaat ggggtccttc
     2701 tggatcaggc tcttgtccac cagggacttg agcagggtcg gcatcaccgg ctccaggccg
     2761 gcggcgagga ccatggcggc gatggagatc gccaccaccc gccagtaggg gacgatggtg
     2821 ccgagcaggc ggcggtagag ctgcagggag gccttcattc gcgccgcagc accttgtcca
     2881 cgaaccaggt gcccaggcgg ctgcgggcat cgaacttttc ggtcagggcc ttctggtcgt
     2941 agcgggtctg cacccaggtg aggaagtcga tgggcgcgcg ggcgttgtcc gcctggtagc
     3001 acgccaggaa gaaatccagg atgccggtgc gggcgatggt gaagtggccg tacttgggcg
     3061 acagctcgcg gatctggtcg aggggttcgc ccaggcggaa gtgacggtag aaggtggccc
     3121 ccagaccggc ccggtcggcg ccggacttgc agtgcatcag cgcgggatag gcgatgcgct
     3181 cgaagaggtc gcgcgcgccc agcacctcct ccaccgaggg catgtcgcgg gagtagaggt
     3241 tgaagttgat cagctcgatg cccagctcgc ggcaggtgtc cacctccaga tcgtaggacc
     3301 cgtgggggtt ctcgccgcgc aggttgatga tcgtcttgat gccgtagagg cggtggtact
     3361 tgcggatctg cgccggactc ggctggctgc accggtacat gccgccgggc agggcgtaga
     3421 agttgttgta gatcgagcgg acgatgccgt ggtcctcgag ctgcatgttc atgtaggcca
     3481 tgaagcggcc gtagggcttg gtgaggtcgt aggccacggg gcgcggggtc cggagggggc
     3541 aaaagcgcga ttatacagac ccttggattt ctctccggca gggcgacggc ttggcgacgg
     3601 gcggtgcggg gatcatactg gactggtcag accatccagg ggagagccac gccatgccac
     3661 gcgcgccggg tcgggaagag gagatggacg aagagggcct ggtgcccttc agcgggttga
     3721 tccgctacga gcgccacgtg ccgattcacc aggtgtccta ctacctgtgc ggtgagatcc
     3781 gtgagcccca gtactacacc gagctgttct acaccctgcg cacggcgtcg gcgacggatc
     3841 tggtgtatct gcacatcaac tcgccgggtg gggatttcaa caccggcctg cagatcatca
     3901 acaacatgct cgcctccgag gcccgcgtgg tgacggtgct ggaggcgcgt gcctattcaa
     3961 tggcggcctt cgtcttcctg tccggcgacg agctcatcgt ccacgacaac tgccagctca
     4021 tgttccacac ctattccggc agctttgcag gcaagggcaa cgagcagctg gccgaggtgc
     4081 aggccctggg cgcgtggttc gagaaggtca cgacgcggat ctgctcgccc ttcctgagcg
     4141 cggcggacat cgaccgcatc ctgaagggca gcgatctgtg gatggattcg gacgagatcc
     4201 gccaccggct cggccggaag gggcgccccg cggggacgcg gcgggggacg cggacggccg
     4261 cgaagcgcct gccggacgtg ccatcggccg actgaggcgg cggcacatcc ggcgacgggc
     4321 gcaggcgcgg gttcagcgtg gccggcgggt acccgccaga ctggcgagac cggcggcgag
     4381 gaggcccagc gcggcgggct ctgggaccgg ctgggtggtg cggctctgaa actggacctc
     4441 gtccacgaag atccacgggc cgttggcgat ggcgccgaca cgcacgtagc gggcgtcgat
     4501 gcccagaccg gccagggtca ggaagtcgtg aggggcggtg ctgaaggggt cgatgttgtg
     4561 agcgctgtcg ggcgggacgg tgaggccggt gacgaaggtc caggtggact tgtcattgct
     4621 gatgtacagc tcgatggacg gcagcaccac gtcgcccagg ttgtcctgcg tcgtgccgac
     4681 gcgcaccaga tcgatgtggc gcgaggtgcc aaagtcgaaa tcgatattga cccggttctg
     4741 atagacccag ccatcccatt cgttgccggc attgacggcc cagccggcga ggcccaggac
     4801 gccgtcggtg agcttggtgt tggcggggtc gtgataacac caggtgccgc aggcggtgga
     4861 ctggtcgtag gtataggacg acggggtgac cgtcgcggca gtcgcggtgc ccgtcagggc
     4921 ggcggtgacc agcacggcgg acagggcatg ctggaaagac gcgcggaaca tcatggctga
     4981 aacccccatg taaattgcaa aattcaggca atttacctaa agcaacagga gtgccaattt
     5041 taacaattcc tttgagttca aggccttgaa aaatgcgagc cggggcgcgt gcggcgcgtt
     5101 gtcagtttat ccgacgcttt ggcgctcagg gcacgggact cccggatgac gggtcgacca
     5161 gggtcaggat gaggtcgtcg ccattgccgc gctggcgaat caggatgcgg tcctggagca
     5221 cccgcacgac gcgcaggtcc ggggcgtatt catgcccctc ggtgaccagc acgaccggcc
     5281 ggccgggctc cttgagcaga gcaatgcccg ggctgtcgcc ggaggggggg atgatgccgc
     5341 gcaggctgag gccggcaggc aggccgtccc cacgggctgg gcgggcggcg taggtggcat
     5401 gcaccggcag cagcagctcc tccgcgtcgg tgcgcggcaa catccacgcg agaagggtgg
     5461 cgaggaggga cccgacgagc acgccggcca gggcgtgcca ccaggtgatg ggcttggggg
     5521 gcctgcggat gggccccgat cgaatgctgt tcatggacgg gcatggcgtt ggggaagcct
     5581 cgatacccta gcatcccact ccccggttct ggcgaccgcg ggcccgcagt tgaagaagtt
     5641 taaccaatgc ggtcgtcttt ttactgaata aaaaacaact ttacttcatg cggttacgtc
     5701 ccctcagcca gcctgcgagt gcggtcccgg cgagcatcag cgcggcactg gagggctcgg
     5761 gcagggcgga cggcgcgagg ccggagtcgg tgctgagcac caggtggtcg aacagcggtg
     5821 tcaggctgtt gtcgatagcg atacgcgtga tgtcggcgtc tcccgactcg gcgtggatca
     5881 gcacataggt ggcgcggccc gaggtgtcgc cccgatagcc ggccaccgtg gacagatcga
     5941 gggtgaagtg cccattgacg gtgacggtct ggccgccgcc gtcgttgtcg gagatgccga
     6001 tgccgaactg gcggatgccc gtcaggctca cggtgaacag cgcattcccc caggtgctgg
     6061 cggtgaggcc gtcccaggcg tgattgggca gggacgcgcc gtaggtggtg aggttggagg
     6121 tcaggccagg gatgaggacg ccgtcctcga agtcctcgat ggcgacgatg ccggtcaggc
     6181 cgagagcgct gtcgagcgcc gcggtgttgg cggtatagac gctggcgtca aaggcggtgg
     6241 cgacataggc gtgggcgggc acggcggcag cgatggcggc ggccagcagg gccgggcgga
     6301 aggggcggtt catggcgatc ctgagcagac gttggcagac ggccctggcg atcttcggtg
     6361 gacggctcga gggcggaacc tctgtgcggg tgcatgacat tcgggcatgc aaatcggatt
     6421 gtaccccggt ggggatcctg tcgggaggac aggatccccc ggatggcagg gcgtcaggcg
     6481 cgccggttga accagcgcag gacggtgccc cagtcgtggc agtcgatgcg ggttgcttcg
     6541 ccgcaggcga agtcgagccc gagccagtgc gtgtcgggca gtcccagcag gtggccggca
     6601 atgacccgca gcgggccgcc gtgggcgacg atggcgaccg cggcggcggg cggtgtcgcc
     6661 tgcaggtcgt ggaggaagtc gagcacccgc gcggccatct ggcgcggcgt ctcgccgccg
     6721 ggcggggcga aatccagggg ggcggcggcc caggcgtcga gggcgctgcc caggtcggcg
     6781 tagcgctgcc cttcccagtc gccgaaatgg atttccatca ggcgcgggtc gagctgcggc
     6841 gtgccgaggg cttcggcgag caggcgtgcg cgtcgcagtg ggctggcaat cagggcgcag
     6901 cccgccggca gcagcgggcg caggcgggcg gcgaccgccg cggcggattc ggcgaggccc
     6961 acgtcggtct gcccgtaaca cacaccgggc gccacgtccg ggcgtgggtg gcggatcagg
     7021 tgaagttcca cgcggcgagc agtgcgaggt agatggcgag ttcggcgatc tgctgggtgg
     7081 cgcccagaca atcgccggtg tagccgtcga ggcggcggcg gaagaggcgg cccgcccacg
     7141 ccgtgacgat gacggtggcg gccagggcca ggccggcttc ggtcgcgtcg aggagcagca
     7201 ggggcagcac gccggcgagg gtggcaatgc ccagctccgc cggcgtgagg cgacgggcca
     7261 cggcgccggc tttggcgtcg tgacctgtgc gggcgtagtc gagcacggcc atcagcgtca
     7321 ccggcgcgag gcgcgacaag gggtgggcag cgaggagggc taggatcacc ggcatgtccg
     7381 cgtcgacgcc cagattgacc agggccaggg ccttgagcag cagggccagg accagcccga
     7441 tggcgccgta gctgccgatg cgcgagtcct tcatgatggc cagggcgcgg tcccggtcga
     7501 ggcccccgcc gaggccgtcg caggtgtcgg ccagcccgtc ctcgtggaag gcgccggtgg
     7561 cgacgagggt cgcgaccatg gacagcacga cggccacgtc gggcggcagc acctggctgg
     7621 ccagcccgta agcgagggcg ccgaggccgc ccaccagcca gcccacggcg ggcaggtagc
     7681 gggccgcatg ggccaggcgc tcgaccgaaa agggcaccca gccgggcacc ggcaggcggg
     7741 tgaagaagcc gaccgcggtg aggaagagtt cgagctgggt gcgcacgggg ctcagtcggc
     7801 cttgccgctg acgccggcgg actcgaagct ggccatctcg ttgaggaaat tcacggccgc
     7861 ctggaccagg ggataggcca gcgccgcgcc ggtgccttcg cccaggcgca gatcgagctg
     7921 caccaggggc tcggcgccca gatggtcgaa ctgtacggcg tggccgggct cctgggagcg
     7981 gtgggcgaag acacagtagt ccagcaccgc cggggcgagg gcgtgggcca ccagcagggc
     8041 gctggtgacg atgaagccgt cgatgagcag caccatgcgg gcctccgccg cgccgagcat
     8101 ggcgcccgcc atcatggcga tctcgaagcc gccgtactgg gcgagggcgt ccagtggatc
     8161 ggcggggcgg ccgccgcggg ccacggcgtg ggccaggagt tcgcgcttgc gggcgaggcc
     8221 ggcgtcgtcg aggccggtgc cgcggccgat cacggtggcc agatcggcgc cagtgaggca
     8281 gtgggtgagc agggacgcgg cggcggtgtt gccaatgccc atctcgccga agcccaccac
     8341 attgcagccg gtgccggcga gacccagggc cagctcgcgg ccgtgggcca gggcggcgtt
     8401 gcgctgggcg gcgtccatgg ccggcgcctc cagatagttg gcggtgccgg gcgccacctt
     8461 ggcggcgatc aggccggggc gcggaccgaa gtcgtgggcc acgcccgcgt ccaccaccga
     8521 caggcccatg ccggcctggc gcgcgaagac gttgatggcc gcgccgccgg cgaggaagtt
     8581 ctccaccatc tgccaggtca cgtcctgggg ataggccgac acgccggcgc gggcggcgcc
     8641 gtggtcaccg gcgaagacca tcacgtgggg ctggcgcagc tccggttcga gggtctgctg
     8701 gatcaggccg atctgcaggg ccagggcctc caggcggcca agggcgccga ggggcttggt
     8761 cttggtgtcg atgcgctgct gcagcgcagg ggcgaggccc cggtcggggc ggatgagggt
     8821 gtagtgcatg atggcgaccg gagaggcgag gctgcggatg ctagcagcgc cggtgccgcc
     8881 gcgtctgcct gacgtagcgt caatcagccg cccttgagga ccagcggcag gccggccgca
     8941 acgaaggtga cgcgcccgca cagggccgcc acggcctgat tgaggcgccc ggcctcgtcg
     9001 cggaagaggc gcccgagggg cgtctccggc accaggccga ggcccacctc gttggccacc
     9061 aggattaccc ggccgggcag ggtgggcagc gcgtcgagga aggcggcgcg ttcgcgggcg
     9121 aaggccggca gcgcctcggc cggcgtggcg ggcggcaggt gctctgcgcc gtcgaggaga
     9181 ttggcgagcc acagggtgag gcagtccacc accgtgcagc ggccgtgggc ggcggtggca
     9241 acgagggccc cggcgaggtc acggggggct tcgacggtgg cccagccggc cgggcggtcg
     9301 gtgcggtggc gggcgatgcg ggcggccatc tcggcgtcca gggcctcggc ggtgacgatc
     9361 acggtgaccg gcaggccgga cgcagcggcg aggtgttcgg cgtggcggct cttgcccgaa
     9421 cgggcgccgc cgaggatcag ttcggggctc atggcaggaa ggggcgcagc ggtgcgctgc
     9481 gggaagtggc tgattccgtg gtattatcgc aggctcgtcg acccgcgggg ctgtctccgc
     9541 aggcgacgag cgtgttccag gtgctcctgc ggccacccgg ccagggagtt aaacgggaaa
     9601 caggtgcgtg gacgtctgcg gacgccacaa agcctgtgct gcccccgcaa cggtaagcag
     9661 atgaacggtg cgtcactccg ccactgggcc ttcgggtctg ggaaggcgac gcaccgggtc
     9721 cggtccggat ttccggccga ccgcctgcca gcccggagac cggcctcgga accctacaca
     9781 cgagcgtgcc gcggggtggc ggtgacggcg tggcccgcgg gccgcctgga tcttctcccc
     9841 gatcgcgcat cacaacgaac gcccggcggc ggggacgcca tcgggcctgg gagaatccga
     9901 ccatgaccat ccgcccgacc ctggctgccc ttgcggtggc tgctgccttt ccgaccgtct
     9961 tttcgtctgc ggccttcgcc gcctccaatg ccccggctgc cgccgcggtg agcagcgccg
    10021 agcagcccgt cgtgctggtg acggccacgc gccagaccca gcgcgccgac gaatccctgg
    10081 ccgacgtcac cgtcatcgaa cgcgcccgca tcgaagaggc cggcgccagc accagcctgg
    10141 ccgagctgct ggcccgccag ccgggcatcc agatgagcag cagcggcggc cccggcaagg
    10201 cgaccgacgt gtacatccgc ggcgccagtc ccaagcacag cctgctgatc gtggacggcg
    10261 tgcgcctggg ttccgcgacc ctcggcgcat cgtcctggca ggacatcccc ctgacccaga
    10321 tcgagcgcat cgagattgtc cgcggcccag cgtccgccct gtacggtgcg gacgccatcg
    10381 gcggcgtgat ccagatcttc acccgccgcg gcgagggtgc ccccaaggcc gacgccttcg
    10441 tcggcgtcgg cagctacggc aacaaggaag tcgccgcagg tgtctccggt gggaccgagc
    10501 gcctgtccta ttccctgcgc ggcgcctaca ccgacgtggc cggctacaac gccatcgcca
    10561 gccaggccaa gcagccttac tactacaacg ccgacaagga tccgtaccgc aacagcagcc
    10621 tctccggcaa tctggcctac acgctgatgc ccggccatga actcggcgcc accctgctct
    10681 acgccacggc ccgcaaccac tacgactccg gcgccggcta cgacgacttc gccgacaacg
    10741 tgcagagcgt ctggagcgcc tacagccgca atcgcttccg cgcgggctgg acgagcacgc
    10801 tgcgcatcgg ccagtcggtg gatgacgccc gcacctacgc ctcctacgcg cccaccgggg
    10861 ccaccatcaa gaccacccag gaccaggtga tgtggcagaa cgacatcacc ctgcccctgg
    10921 gcacgctgct ggcggcgctg gaaaacctgg agcagaaggc cgtcaacgaa ggcaccttct
    10981 cggtcagtcg ccagatccgt tcggccctgc tgggctggac cgccagcgtg ggcgagcacc
    11041 gcatgcagtt caacgcgcgg tatgacgaca actcccagtt cggtaacaag accaccggct
    11101 acgctggcta cggctaccag atcctgccca gcctgcgcgt gcagggcagc gtcggttccg
    11161 ccttccgtgc gccgaccttc aacgagctct acttcccggg cttcggcaat cccaacctga
    11221 agccggagaa cgcctataac aaggagctgg gtctgctgtg ggaacagcac gggcagagcg
    11281 tgagcctgac cgccttccac aaccgcatca ccaatctcat cgcctacgac gccaatttca
    11341 tcccccagaa cgtcaataac gcgcgcctgc agggggtgag cctgggctat gccgggacac
    11401 tcgccggctt cgacatcaac gccaacctcg acttcctcga tccggaggac gccgacaccg
    11461 gcaagcgcct gcagcggcgt gccgacaaga ctggcaacct gtacctcagc cgccgcatcg
    11521 ccgccttcaa cgtgggcgcc gaatggcagg gtgcggccgg ccgctacgac cggacgggcg
    11581 agatcaaccg catgggcggc tacgggctgg tcaacgcctt cgtgaagtac gcggtggatc
    11641 gggactggag cctggaggcc cgcgccaaca acctgctcaa caagcagtac gagaccgcct
    11701 ggggctacaa cacggcgggc gcgaacctgt tcttcggcgt acgctacgcc cccaggtaag
    11761 gcacgatgaa cggaggggcc atggcgcagg cggttccggc gggccgcacg acagctggcg
    11821 gcatggggcg cgtgcgacaa cgcgcggccc tggtgctgct ggtcctcgtc gctgcggcgc
    11881 tggcagcctt cgcctgggcc ctgtcggcgg gggagctgcc ggtgacggct gcggacctgc
    11941 tggcggccct cggcggtggc gcgggcagca tggaggccga catcatccgc accctgcgcc
    12001 tgccccgcgc cgtggcggcc ttcgcctgcg gcggcctgct cgccctgtcc ggtgcgctga
    12061 tgcaggtgct gctgcgcaat cccctggccg atccttacgt gctgggcgtc tccggcggcg
    12121 ccgcggtggg cgccctgggc tgcctgctgg cgggcgtcgc ggggctgtgg atcgacgtcg
    12181 gtgccttcgc cggcgccctc gtcgccaccc tgctggtatt cggtctggcc cgcggagacg
    12241 gttcgtggac gcgcacacgg ctgctcctca ccggcgtcat cgtcgccgcc ggctgcggcg
    12301 cggcggtggc cctgatgctc agcatcgcgc cggaaggtcg cgtcaagggc atgctgttct
    12361 ggctgatggg cgacgccagc ggcgtctcga acccgtggcc ggccctcggc gtgctggccg
    12421 tgggcttgct gaccgcgctg cccttcgccc gggagctcaa cctgctggcc cgtggcgaag
    12481 tcaccgcccg cgccctcggc gtacgggtcg ggcgtctgcg tgccctggtc tacgggctcg
    12541 gcgccctgct caccggcgtg gcggtgacca ccctgggctc ggtgggcttc gtcggcctga
    12601 tcgtgcccca tctggtgcgc ctggtcattg gcaatgacca gcgcctgctg ctgcccgccg
    12661 cgagcctggc aggcggcgtc ctgctcaccc tggcggacac cgccgcgcga accgtggtgg
    12721 cgccggtgca gctgcccgtg ggcgtgctca ccgcgctgat cggcgtgccc gtcttcctct
    12781 tcctgctggc gcgtcatccc cgatgaagat ggacgtgcgc cccgacgtgc tggccgccga
    12841 ggccctcgcc gtgcaggtgg gccggcgcga cgtggtgcgc ggtctggagg tgcgcgtcgc
    12901 gcccggcgat accctggtga tcctcggccg caacggtgcc ggtaagacca ccctgctgca
    12961 caccctggcc ggcctgcgtc ccgcggcggg gggcacggtg ctgctctccg gcaaacccta
    13021 cgccgactgg ccgcggggcc acgaggcctg cctgcgcggc ctgctgcccc agagccagcc
    13081 ggatttcttc agcgccacgg tgctggagac ggccctcgtc gggcgccatc cccacctcgg
    13141 ccgctgggcc tgggaagggc cggacgacga agccatcgcc ctcgccgcgc tggccaccgt
    13201 ggggctcgcg gagtacgccg cccgcccggt gctgaccctc tccggcggcg aacgccagcg
    13261 cctcgccatt gccgcgctgc tggtccagca gccgcgcctg tacctcctcg acgagcccct
    13321 ggcacacctg gacctgcatc atcagatcgg cgtgctcgat cactttcacc gcctcgccgc
    13381 cgacggtgcg ggcgtggtga tggtgttgca cgacatcaac ctggccgccc gctacgccag
    13441 tcacgtgctg ctgctggacg gtgccggtgg ctttgcctgc ggaccggccc gcgacatcct
    13501 caccacggcc cgcctctcgg ccgccttcgg tcatcccctg cgggccgtcg cggacggagc
    13561 gagcaccctg ttcgtgcccg cctgagcgct tttcgccgca gtgcggccaa cggcccccgc
    13621 taacttttag cgattcccga gtcctgccca tgaatgaacc gacgccgccc gctccacccg
    13681 ccgatcccga ccgcgacgcc cgccacaccg cccgcatggc ccgcaagaag gctgtggtgg
    13741 atgccgccat cgctgccgcc accgaacggc gcggcgtgct cctggtgaac accggcaacg
    13801 gcaagggcaa gtccagcgcc gccttcggcg tcctcgcccg ggccctcggc cacggcctgc
    13861 agtgcggcgt cgtgcagttc gtgaagggcc gctcggacac cggcgaggag gccttcttcc
    13921 gccgccagcc cgaggtgcgc tggcacgtga tgggcgacgg cttcacctgg gacacccagg
    13981 accgggccgc cgacatggcc agcgcccgcg ccggctggga aaaggcccgc gccttcctgc
    14041 gcgatccggc catcggcctg gtggtgctgg acgaactcaa catcgtcctc aagtacgcct
    14101 acctggacgt ggccgaggtc gtcgccgacc tgcgcgcccg gccgccgcag cagcacgtca
    14161 tcgtcaccgg ccgcggggcg cccgcggcgc tgatcgacgt ggccgacacg gtgaccgaga
    14221 tgggcccggt caagcacgcc ttccaggccg gcgtggcggc ccaggcgggc gtggagttct
    14281 gaagggcccg cctcggcatc cggctgcgtc ccggcctgcg tgcagggcgg gcgtagacga
    14341 ggccggtgga tggaccttca gccgcttgca cgggcggacg gcccgcggcc gccgcaggct
    14401 gaaggatccg cccgcatggg cagccgcccg gccgcgctcc ggctgggtta agctgcgcag
    14461 ctcccgaacc tgcctgtcga cccatgcatc gcatcgtcgt ccttgaagcc ggcgccgtca
    14521 tcgccaccct gcgccgcccg gcctttgccc acgaatgggt cgaatacgcg gcgacgcgtc
    14581 aggagcaggt ggccgagcgt ctggccggtg ccaccatcgc ggtggtgaac aagcgccagc
    14641 tcggtgcgga gctcctcgcc ggcctgccca cgctgcgtct gatcgccatc gctgccaccg
    14701 gctacaacaa cgtggatctg gaggcctgcc gcgcgcgggg catcgtcgtt tgcaacgtcc
    14761 gtggctacgc ccgccagacc gtgcccgagc acgtcttcgc cctgctgctg gccctggcgc
    14821 ggcagatcgg cccttatcac cgctcggtcg cggccgggcg ctggcagcgc agcgagcagt
    14881 tctgctactt cgactatccg atccgcgatc tggccggaca gaccctcggc ctgctcggcg
    14941 ccggcagtct gggggaaggc gtggcgcgca tcgccgcagc cttcggcatg cacgtgctgc
    15001 ggggcgagcg caagggcgcc gccagcgtgc gtgagggcta cgtggacttt gacacgctga
    15061 tccgcaccgc cgacgtgatc agcctgcact gccccctcac ggaggccacc cggcatctca
    15121 tcggcgcggc cgaactggcc gccatgaagc cgacggcgct gctcctcaac accgcccgcg
    15181 gcgggctagt ggacgaggcg gccctggccg aggccctgcg cgcccggcgc atcgccggtg
    15241 ccggcttcga cgtgctcagc gaggagccgc cgcgggacaa tcccctgctc gcgccggacc
    15301 tgctggaagc cggccgcttc ctcctcacgc cccatgtggc ctggtccagc cgggaggcca
    15361 tgcaggccct ggccgaccag ctgatggaca acatcgacgc ctttgccgcg ggcacgccgc
    15421 gcaaccggct ggcctgaggc ggacgctgcg tcaggccggc accgtcaccg gcagcgcccg
    15481 gtccaccacg atttcgtccg gcgacacccg cgcaccgagg gcgagcacct cgacgcccgc
    15541 agcgagggcc tcgcgcaggg cgcgcccgta ggccgggtcg atgtggtccg ctgggcgcac
    15601 cgccgccacg tccgcccgct gcacgcagaa gaccagcacc gcgcgccggc cgacggccac
    15661 catggcggcc atctcgcgca gatgcttggc gccgcgggtg gtgaccgcgt cgggaaagta
    15721 gccgacgccg ccgctcaccg ccgcagtgac gttcttcacc tccacgtagc agtccggccg
    15781 gccgtcgccg ctcaggagga ggtcgatacg gctgccttcg ccgtacttca cctcgccgcg
    15841 aatggtcggg tagccc
//
LOCUS       DAOQPA010000085        15851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1871_length_15851_cov_17.915105, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000085 DAOQPA010000000
VERSION     DAOQPA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_15851_cov_17.915105"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1382)
                     /locus_tag="PKX01_11975"
     CDS             complement(246..1382)
                     /locus_tag="PKX01_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ76892.1"
                     /translation="MILTQEQEMIRDSMRAFSQERLAPFAGEWDKHHTFPAQALKELG
                     ELGALGMVVPEEWGGAGMDYMSLVLTLEEIAAGDGATSTIVSVQNSLACGITMKYGTD
                     AQKEEWLKPLARGEKLGCFCLTEPHTGSDAAAITTRAEKDGDHFVLNGVKQFITTGKH
                     AHMAIVFAVTDKAAGKKGISCFLVPTATPGFIVGRTEEKMGQHASDTVQIIFENCRVP
                     ASALLGKEGEGYRIALSNLEAGRIGIAAQSIGMARAAFEAAVRYARERVTFGVPIIEH
                     QAVNFKLADMNTLLDAARLMVWRAAQLKDAGKPCLKEASMAKMFASEAAEKIASDAIQ
                     IHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRLVIGRSIAAE"
     gene            complement(1742..2932)
                     /locus_tag="PKX01_11980"
     CDS             complement(1742..2932)
                     /locus_tag="PKX01_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HMZ76893.1"
                     /translation="MSTANDPVVIVSAARTPMGGFQGDFSSLTAAQLGSVAIKAAVQR
                     AGISAEAVEEVIFGCVLPAGQGQAPARQAALGAGLSLSAGCTTINKMCGSGMKATMLA
                     HDLLLAGTNEVMVAGGMESMTNAPYVLPKARGGYRLGHGQIFDHMFLDGLEDAYEKGR
                     LMGTFAEDCAGLYQFTREQQDAYAVASLTRAQAAIRDGAFAWEIEPVTLAGRKGEILI
                     KDDEQPPKANLEKIPSLKPAFRKDGTVTPANSSSISDGAAALVLMRASSAERMGLKPL
                     ARIVAHTTHAHQPSLFATAPVGAMQKLLAKTGWSVADVDLWEINEAFAVVAMAAIHDL
                     QLDHAKVNVHGGACALGHPIGASGARILVTLLGALRKTGGKRGVASLCIGGGEATALA
                     LEML"
     gene            complement(2988..3752)
                     /locus_tag="PKX01_11985"
     CDS             complement(2988..3752)
                     /locus_tag="PKX01_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ76894.1"
                     /translation="MEIQNNVFVVTGGGSGLGAATARQIVARGGKVVLADVNAQAGEA
                     LAAELGAAARFVATDVTDEASAQAAFAAATAMGPLRGLVNCAGVAPAEKVVGKEGAHK
                     LASFTRTITINLIGSFNMIRLAAEIMSKSEPDEGGERGVIVSTASVAAFDGQLGQAAY
                     AASKGGVVAMTLPIARELSRAGIRVMTIAPGIMETPMLLGMPADVQDALGKMVPFPSR
                     MGKPAEFAALVEHILGNRYLNGEVIRLDGAIRMAAK"
     gene            3983..5005
                     /locus_tag="PKX01_11990"
     CDS             3983..5005
                     /locus_tag="PKX01_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ76895.1"
                     /translation="MNAPPPDKGTISIHFVQEALEGVVQRGLDAEDLLNRAGISPELL
                     NAPQARVSSAHFGALWHLIAQALDDEFFGMDSHPMRVGSFTLLCHALIHCETLEKALR
                     RALRFLRLVLDDLSGTLVRDGDRAYLVIADRCSAPRRAFAYGTYLIILHGLACWLIGR
                     RIPLQEARFRCAEPAFSGEWRVLFSPDLRFDQAETAIVFPADYLDMANVQNERTAKVF
                     LRSAPANFLVKYRNSEGLTARIRRLLRRLPPEAWPDFDTLARQLHSSASSLRRHLTDE
                     GQSYQSIKDDLRCDLAISQLSHSDKSVLDIAHELGFAEASAFHRAFKKWTGARPGEYR
                     RALAGH"
     gene            5017..7944
                     /locus_tag="PKX01_11995"
     CDS             5017..7944
                     /locus_tag="PKX01_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMZ76896.1"
                     /translation="MPDKGARGRGARWCLPLHIHISTLFFLLVLMAGGSIAWVSYTRS
                     ADMMERAAADLLGRISRQIAAETETLLKPVGASVRLLARSPVTRAGTLETRREQLASF
                     REALLASPSASAFYIGYEDRDFFLLLRLANRSDRDLMGAPPGAAYLLQSIDHGSGRFV
                     FLDEDLATLRSDPRPDYGTAYDPTRRSWYGQALAGPEPIQTEPYLFASTRKLGVTVAR
                     HTDTGRSVVGADIRLETLDESLRRQRITPGSQLVLFDRDLGVLAYTGKDWLPPPQAGE
                     ARRPDLTELGGGVLKALAQRLPQDGETEPLGSFRAAGRDWHGAVAQVQWLGGQPVYLG
                     VVIPDEELLVEARGIRNQSISVTLLVMLLALPFTYLAARLVSRPIRDLAGETAAIRRF
                     DFSSPVRTRSVVREVDNLATDLGSMKGAIRRFLDIASAMAEESSFERLLPRLVDETVA
                     VVGARAGVLYLAAHGGEALDAVVLRDADGASHPLEQAPAGATMAGLVPAGARSGVGRL
                     TLQTAAGAASQTLFGLLGAEDLAYLAVPLTDRKQARLGLLVLWFDAPPDRDLVHFVEA
                     LSGSAAMSVETRALIRAQKELFEAFIQLIAGAIDAKSPYTGGHCARVPELTKMLAQAA
                     CNSADGPFRDFRLGEEDWEAVHIAAWLHDCGKVTTPEYVVDKATKLETLYDRIHEIRM
                     RFEVLKRDATIAYWQARAEGADEAAAKGRLVETHAALDREFAFVAACNEGAESMAPED
                     VARLQQIARRTWQRTLDDRLGLSPEERRRRADAGPVRLPVTEPLLADKPEHRYERGAM
                     DLLPADNPWGFRLKVPALLYNRGELTNLSITRGTLTDEDRYKINEHIVYTIRMLSELP
                     FPRHLRAVPEIAGGHHEKMDGSGYPRGLKGGEMSLVARMMAIADIFEALTAVDRPYKT
                     GKRLSEAVSIMAGMRDRGHIDPDLFALFLRSGVYRRYAERFMQRKCVDEVDVDAMLAE
                     VTA"
     gene            complement(7941..8228)
                     /locus_tag="PKX01_12000"
     CDS             complement(7941..8228)
                     /locus_tag="PKX01_12000"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMZ76897.1"
                     /translation="MAPGCAAPRRLHGSLPMTEPQIAQYASYTVPVEAGREYTWCACG
                     RSATQPFCDGRHAGSGIAPKTFTPLRSGLAYLCGCKHSRRAPFCDGTHDTL"
     gene            complement(8241..8807)
                     /locus_tag="PKX01_12005"
     CDS             complement(8241..8807)
                     /locus_tag="PKX01_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ76898.1"
                     /translation="MKKLTALILAAGLSTAALAAPESYTIDGSHTFPRFEYSHFGYST
                     QLSRFDKTTGTIVLDRAAKSGTVDVTVDATSVDTGFPLLNQHIQGEDFFDTAKYPTIT
                     YKSTKVNFDGDKVASVEGNLTVKGVTKPVTLTVTSFHCMPHPMLKKDACGANAVAKIK
                     RSEFNAGKYAPYVGDDVTLTIAVEAYKP"
     gene            complement(8878..9456)
                     /locus_tag="PKX01_12010"
     CDS             complement(8878..9456)
                     /locus_tag="PKX01_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ76899.1"
                     /translation="MKTLRPTLTLALLAGLTTGAAHAADFTQVLTDKSSLAFTSKQMG
                     VPVDGLFRKFSAQLAFDPAKPATASTVLEIDLASIDAGSKDANEEVVGKQWFNVKAFP
                     TARFVSTAVKAVGGGRYEVSGKMTIKGQTHDVVAPFTFRQDGANGVFDGGFVLKRMDF
                     GIGEGPWSDVSAVANEIQIKFHVVATPAPTKK"
     gene            complement(9485..10021)
                     /locus_tag="PKX01_12015"
     CDS             complement(9485..10021)
                     /locus_tag="PKX01_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HMZ76900.1"
                     /translation="MNTERYSSPAIAAHWLIALLIFAAFPLGLYMHELPLSPTKLKLY
                     SYHKWIGMTVLGLFVLRVLWRLTHRPPAPLPAPAWQQTAAEVTHRLLYVLMALVPLTG
                     WLMSSAKGFQVVYLGVLPIPDLIAKNPDLGEILEEVHESLNWLLFGLVGLHVAAALKH
                     HLIDKDATLRRMLPFGRV"
     gene            complement(10024..10839)
                     /locus_tag="PKX01_12020"
     CDS             complement(10024..10839)
                     /locus_tag="PKX01_12020"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HMZ76901.1"
                     /translation="MSARHPLLFVSHGAPDVLLRPAATVALWADLGRRLPRPRAILVV
                     SAHWAARQPTVSTVSMPATIHDFGGFPEPLYAMEYPAPGAPDVAARVRDALVAAGLPC
                     AEVPDRGLDHGAWIPLKAMYPAADIPVVQLALQPHAGPDWHRRIGTALRGLRDEGVLI
                     LASGAITHNFGWLDWSGGGEPLPQAKAFADWVGAAVARNDRPTLLEYRRQSPLGAAAH
                     PSEEHLLPLFVTLGAADPDEIALRLEPEFTYGGLAMDAYLWQKDSIIPYTPEP"
     gene            complement(10836..11780)
                     /locus_tag="PKX01_12025"
     CDS             complement(10836..11780)
                     /locus_tag="PKX01_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HMZ76902.1"
                     /translation="MKAYSGTQFINPPQESSVILVTGANGRLGRRIVQHLIARRPLRD
                     APALAVSVRQPALAADLAARGVDVRAGDFDQPETLAATFAGVRTLVLVSTDGPSAQRI
                     AQHAAAISAARRAGVQRVLYTSFLDVGTDSPAEFARVHRQTEDALAASGLDATILRNP
                     LYADDLPALVVPGPDGDAFCLAAGNGAVSLVSRDDLARALAAAALAPRLDKRVYELTG
                     SEAVAYAELAARIARVSGRPLRYLPVTVTEQAATLRAAGLPDGLAEGVASLFAAVAEG
                     RLARVTHDFAALVGHPPRSLDCLIREYFAPVTAPRTAS"
     gene            11831..12745
                     /locus_tag="PKX01_12030"
     CDS             11831..12745
                     /locus_tag="PKX01_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ76903.1"
                     /translation="MMDRLQAMEVFVRVAEAGSFAAAADQLDLARSVVTRLVAALEAR
                     LGVKLIARSTRSLRLTSAGQAYLERCREILDLVGVAEGELGGNERAPRGPIRLSVPVS
                     FGIHHLAPLVADFVTTWPDVSLDIDFNDRQVNLVEAGVDMAIRITTQLDPSVVARRLT
                     VSRMLTLAAPEYLARHGRPVRPDDLLDHPCLGYTGTARASWPYRVAGTLQWVPVRGRL
                     QSNNGDALLDAARRGLGICLQPSFIAAAAVQAGLLEVLLADFPGPELGIYAVFPSHRY
                     VPSRVRLLADYLAARIGPVPYWEQGLPA"
     gene            12880..13383
                     /gene="tpx"
                     /locus_tag="PKX01_12035"
     CDS             12880..13383
                     /gene="tpx"
                     /locus_tag="PKX01_12035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HMZ76904.1"
                     /translation="MSSTVTLGGNPVTVAGDLPRVGATAPAFSLVAKDLSEVTLANFA
                     GQRKVLNIFPSIDTPTCATSVRKFNQSASSLPNTAVLCISADLPFAQNRFCGAEGLSN
                     VVTLSTLRGREFLEAYGVALASGPLAGLAARAVVVVDEQDVVRHVELVPEIKNEPDYA
                     AALAALG"
     gene            13580..14659
                     /gene="ada"
                     /locus_tag="PKX01_12040"
     CDS             13580..14659
                     /gene="ada"
                     /locus_tag="PKX01_12040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HMZ76905.1"
                     /translation="MTASTSSPPTAADPRWAAVQARDGTADGHFWYAVTTTGVFCRPS
                     CPSRRPRPEHVRFFDSAAAAREAGFRPCLRCHPDTLADPHVALIATVCRAIQAAETPP
                     SLRALSVLAGLSAGHLQRVFKAATGLSPRAYAAALRAGRVRTGLAAGAPVTHAVLDAG
                     FGSTGRFYARSAQVLGMAPAAWRAGGAGEDIRFAVGACELGALLVAASPRGICALSLG
                     DDPDALVRALQDRFPRACLVGDDPAFATWVSRVAGYLQAPRDGLELPLDLRGTAFQLR
                     VWHALQGIPPGATISYAELARRVGAPRAVRAVAGACAANPVALAVPCHRVVRSDGSLS
                     GYRWGVERKAALLTREAERGGRDDA"
     gene            14646..15299
                     /gene="alkB"
                     /locus_tag="PKX01_12045"
     CDS             14646..15299
                     /gene="alkB"
                     /locus_tag="PKX01_12045"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HMZ76906.1"
                     /translation="MTTPDLFEGWTPEDVGPEALSPGAVHLRGFVRAEAAAVLAELDR
                     VLAHAPLRTMQTPGGRSMSVATSNCGARGWVSDAAGYRYAPADPLGGAAWPTMPRLWR
                     TLAERAAAAAGFGGFTPDACLINRYAPGARMGLHQDRDEADFTWPIVSFSFGLPAIFL
                     FGGLRRGDPARRISLFHGDAVVWGGPSRLAFHGVAPVKPGEHPLTGACRINLTFRRA"
     gene            complement(15363..15439)
                     /locus_tag="PKX01_12050"
     tRNA            complement(15363..15439)
                     /locus_tag="PKX01_12050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15403..15405),aa:Ile,seq:cat)
     gene            complement(15558..>15851)
                     /locus_tag="PKX01_12055"
     CDS             complement(15558..>15851)
                     /locus_tag="PKX01_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ76907.1"
                     /translation="LGDFIEDNATMAPADAALYSSLRDATKEVLDSLTPREAKVLRMR
                     FGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDNEA"
BASE COUNT         2180 a         5211 c         5863 g         2597 t
ORIGIN      
        1 cggacagccg cgccggaagg ccccgcccac tgctccgctg cgtggcgctt caggcgggct
       61 ttggccggag gccgggcctc ttcgggacct ggggcgaatg atgttcgccc cgacaacgct
      121 cgcatcgtaa agggtgcgca gggcgttgcg agcggtccga ggttgcgacg aggaacgcag
      181 tcgtactcgc gtacggcgcg taccggaatc gcaagctcgg gccgcgcagt cgcccggcgc
      241 accgtttact cggcggcgat ggagcggccg atcacgagcc gctggatgtc gttggcgcct
      301 tcgtagatct ggcagatgcg cacgtcgcgg tagatgcgct ccaccgggaa gtcgctggtg
      361 tagccgacgc cgccgtggat ctggatggcg tcggaggcga tcttctcggc ggcctcggag
      421 gcgaacatct tggccatcga ggcttccttc aggcagggct tgccggcgtc cttgagctgg
      481 gcggcgcgcc acaccatcag gcgggcggcg tcgagcaggg tgttcatgtc cgccagtttg
      541 aagttcaccg cctggtgctc gatgatgggc acgccgaagg tcacccgctc gcgggcgtag
      601 cgcacggcgg cttcgaaggc ggcgcgggcc atgccgatgc tctgggcggc gatgccgatg
      661 cgcccggctt ccaggttgga cagggcgatc ctgtagcctt cgccttcctt gccgagcagg
      721 gcagaggccg gcacgcggca gttctcgaag atgatctgca cggtgtcgga ggcgtgctga
      781 cccatcttct cctcggtgcg gccgacgatg aagcccggcg tggcggtggg caccaggaag
      841 caggagatgc ccttcttgcc ggcggccttg tcggtgacgg cgaagacgat ggccatgtgg
      901 gcgtgcttgc cggtggtgat gaactgcttg acgccgttca gcacgaagtg gtcaccgtcc
      961 ttttcggcgc gggtggtgat ggcggcggcg tcggagccgg tgtggggctc ggtgaggcag
     1021 aagcagccga gcttctcgcc acgggccagg ggcttgagcc actcttcctt ctgggcgtcg
     1081 gtgccgtact tcatggtgat gccgcaggcc agggagttct gcaccgaaac gatggtggag
     1141 gtggcgccgt cgccggcggc gatctcctcc agggtgagca ccaggctcat gtagtccatg
     1201 ccggcgccgc cccattcctc gggcaccacc atgcccaggg cgcccagttc acccagctcc
     1261 ttgagggcct gggcggggaa ggtgtggtgc ttgtcccatt cgccggcgaa gggggcgagg
     1321 cgctcctggg agaaggcgcg catggagtcg cggatcattt cctgttcttg ggtgaggatc
     1381 atggtgtggt gtcctttggt gtctcttgcg ttgttcgtgg ggccgtttga gcgggctggc
     1441 ggttcgctgg cgcgcccttg caggacggct ttccgttgtc gggtgttgcg ttggccgggt
     1501 ctcgccccgg cttcacgggt cggcctacgg ctgactcccc tgcgcggctc acaacaggcg
     1561 gccggcgcag aactcgtcct ccgggctcag acagctgtgc cggaaatccc cgccgtttgc
     1621 tgagctgccc ggcggtccag acgggctttg gccgggcacc gagcctttgt tggctgggcg
     1681 ctgcgatcct gtaggtcggg ttcgcctgca gggcgtaacc cgacgtcgga ggctgcctgc
     1741 cttacagcat ttccagcgcc agggccgtgg cttcgccgcc gccgatgcac agactcgcca
     1801 cgccgcgctt gccgccggtc ttgcgcaggg cgccgagcag ggtgacgagg atccgtgcgc
     1861 ccgaggcgcc gatggggtgg ccgagggcgc aggcgccgcc gtggacgttc accttggcgt
     1921 ggtccagctg taggtcatgg atcgcggcca tggcgacgac ggcgaaggct tcgttgatct
     1981 cccacagatc cacatcggcc acgctccacc cggtctttgc cagcagcttc tgcatggcgc
     2041 cgaccggggc ggtggcgaac aggctgggct ggtgggcgtg ggtggtgtgg gccacgatcc
     2101 gtgcgagggg cttgagaccc atccgctcgg cgctggaggc gcgcatcagc accagggcgg
     2161 cggcgccgtc ggagatggag ctggagttgg ccggcgtcac ggtaccgtcc ttgcggaagg
     2221 cgggcttgag gctggggatc ttttccaggt tggccttggg cggctgttcg tcatccttga
     2281 tcaggatttc gcccttcctg ccggccaggg tgacgggctc gatttcccag gcgaaggcgc
     2341 cgtcgcggat ggcggcctgg gcgcgggtga gggaggccac ggcgtaggcg tcctgctgct
     2401 cgcgggtaaa ttggtagagg ccggcgcagt cctcggcgaa ggtgcccatc aggcggccct
     2461 tctcgtaggc gtcttcgagg ccgtcgagga acatgtgatc gaagatctgg ccgtggccca
     2521 ggcggtagcc gccgcgggcc ttgggcagca catagggcgc gttggtcatg gactccatgc
     2581 cgccggcgac catgacctcg ttggtaccgg cgaggagcag gtcatgggcg agcatggtgg
     2641 ccttcatgcc ggagccgcac atcttgttga tggtggtaca gccggccgac agggacaggc
     2701 ctgcgcccag ggcggcctgg cgcgctgggg cctggccctg accggcgggc agcacgcagc
     2761 caaagatgac ttcctcgacc gcttcggcgg agatgccggc gcgctggacg gcggccttga
     2821 tggcgacgct gcccagctgg gcggcggtga ggctggagaa atcgccctgg aagcccccca
     2881 tcggggtgcg ggcggcggag acgatgacga cggggtcgtt ggcggtgctc atggggaaaa
     2941 cctttctcaa ttcgggagga gatgggatcg cgatgcgccg gggcgcgtca cttggcggcc
     3001 atgcggatgg cgccgtcgag gcggatcacc tcgccgttga ggtagcggtt gccgaggatg
     3061 tgctccacca gtgcggcgaa ttcggccggc ttgcccatgc gcgaggggaa ggggaccatc
     3121 ttgccgaggg cgtcctggac gtcggcgggc atgcccagca gcatcggggt ttccatgatg
     3181 cccggggcga tggtcatgac gcggatgccg gcgcgggaca gctcgcgggc gatgggcagg
     3241 gtcatcgcca ccacgccgcc cttggacgcg gcgtaggcag cctggccgag ctggccgtcg
     3301 aaggccgcca ccgaggcggt ggacacgatc acgccgcgct cgccgccctc gtcgggctcg
     3361 ctcttgctca tgatctccgc ggcgaggcgg atcatgttga agctgccgat caggttgatg
     3421 gtgatggtgc gggtgaagga cgccagcttg tgggcgcctt ccttgccgac gaccttttcg
     3481 gccggggcca cgccggcgca gttcaccaga ccgcgcaggg ggcccatcgc ggtggcggcg
     3541 gcaaaggcgg cctgggcgct ggcctcgtcg gtcacatcgg tggccacgaa gcgggcggcg
     3601 gcgcccagct cggcggcgag ggcttcgccc gcctgggcgt tgacgtcggc cagcaccacc
     3661 ttgccgcccc gggcgacgat ctggcgggca gtggcggcgc ccaggccgga accgccgccg
     3721 gtgaccacga atacgttatt ttggatctcc atgcattttc tcctcttcgg tcttgtggat
     3781 tgtcgggtcg aggctgcaga cccggtgacg atgcgagcgc cagtctaggg gctcgcccgg
     3841 tcggcttcca tgccgaaatc ggtcaatgtg aatggtggga tttgccatac ccccggccgg
     3901 gcgggcggtg gttgcctgcg caccggtgtt ggtgggtggt atcctcgctg cgccgatcct
     3961 ccatgccagt tgcaaacgcc cgatgaacgc gccgccaccc gacaagggca ccatctccat
     4021 ccatttcgtc caggaggcgc tggaaggggt ggtccagcgc gggctcgatg cggaggatct
     4081 gctcaatcgg gccggtattt cgccggaact cctcaacgcg ccccaggcgc gggtgtcctc
     4141 ggcccacttc ggcgccctgt ggcacctcat cgcccaggcc ctcgacgacg aattcttcgg
     4201 gatggacagt cacccgatgc gggtgggcag ctttaccctg ctgtgtcatg cgctgatcca
     4261 ctgcgagacc cttgaaaaag ccctgcgccg cgccctgcgt ttcctgcggc tggtgctcga
     4321 cgacctgtcc gggacgctgg tgcgcgacgg cgaccgggcg tacctcgtga ttgccgatcg
     4381 ctgcagcgcc ccccgccggg cctttgccta cggcacctat ctgatcatcc tgcacggcct
     4441 cgcctgctgg ctgatcgggc ggcgcatccc gctgcaggaa gcccgcttcc gctgcgccga
     4501 gccggcgttc agcggcgaat ggcgggtgct cttttcgccc gacctgcgtt ttgaccaggc
     4561 ggagaccgcc atcgtctttc cggcggacta cctggacatg gccaatgtgc agaacgagcg
     4621 cacggccaag gtcttcctgc gcagcgctcc ggccaacttc ctggtcaagt accgcaacag
     4681 cgaaggcctc acggcgcgca tccggcgcct gctacggcgc ctgccgccgg aggcctggcc
     4741 ggatttcgac accctcgcgc gccagctgca cagctccgct tcgtccctgc ggcgccacct
     4801 gacggacgag gggcagtcct accagtccat caaggatgat ctgcgctgcg acctggcgat
     4861 cagccagctc agccactcgg acaaatcggt gctggatatc gcccatgaac tgggcttcgc
     4921 ggaggcaagc gcctttcacc gggcgttcaa aaaatggacc ggcgcccggc cgggcgaata
     4981 ccgccgtgcc ctggcgggac actgaaggag gagacgatgc cggacaaggg ggccaggggg
     5041 cgcggcgcac gatggtgcct gccgctgcat atccacattt ccacgctgtt cttcctgctg
     5101 gtgctgatgg ccggcggcag catcgcgtgg gtgtcctaca cccgcagtgc cgacatgatg
     5161 gagcgggccg ccgccgatct gctggggcgc atctcgcggc agatcgccgc cgagacggaa
     5221 accctgctca agccggtggg ggcctcggtg cgcctgctgg cccgctcgcc ggtgactcgg
     5281 gcgggcaccc tggagacgcg ccgcgagcag ctggcgagtt tccgcgaggc cctgctcgcc
     5341 tcgccgtccg cttcggcctt ctacatcggc tacgaggacc gggacttctt cctgctcctg
     5401 cgcctggcca accgctccga ccgggacttg atgggagcgc cgccgggggc cgcctacctg
     5461 ctgcagagca tcgaccacgg cagcggccgc ttcgtgttcc tcgatgagga tcttgccacc
     5521 ctgcgtagtg acccgcgccc cgattacggc accgcctatg accccacccg gcggagctgg
     5581 tacggccagg ccctggccgg ccccgagccg atccagaccg agccttacct gttcgcctcc
     5641 acccgcaagc tgggcgtcac cgtcgcccgt cacacggaca cgggccgctc ggtggtgggg
     5701 gccgacatcc gcctggaaac cctcgacgag tcgctgcgcc gccagcgcat cacaccggga
     5761 tcgcagctgg tgctcttcga ccgcgacctg ggcgtgctgg cctatacggg caaggactgg
     5821 cttccgcctc cacaggcggg cgaagcgcgc cggccggatc tgacggagct gggcggcggc
     5881 gtgctcaagg ccctggccca gcgactgccc caggacggcg agaccgagcc cctgggcagc
     5941 ttccgcgcgg ccgggcggga ctggcacggc gccgtcgccc aggtgcaatg gctgggcgga
     6001 cagccggtgt acctgggcgt ggtgattccg gatgaggagc tgctcgtgga ggcgcgcggc
     6061 atccgcaatc aatccatcag cgtcaccctg ctggtgatgc tgctggcgct acccttcacc
     6121 tacctggccg cgcgcctggt gtcgcgcccg atccgcgacc tggccggcga gacggcggcg
     6181 atccggcgtt tcgatttctc cagcccggtg cggacccgtt cggtggtgcg cgaggtggac
     6241 aatctggcca ccgatctggg ctccatgaag ggggcgatcc ggcgcttcct cgacatcgcc
     6301 tcggccatgg ccgaggaatc cagtttcgag cgcctgctgc cgcgcctggt ggatgagacc
     6361 gtggccgtgg tgggggcccg cgccggtgtg ctctacctgg ccgcccacgg cggcgaggcg
     6421 ctcgatgcgg tcgtcctgcg ggacgcggac ggtgccagtc atccgctgga gcaggctccg
     6481 gcgggggcga cgatggcggg tctggtgccg gcgggggccc gctccggcgt gggacgcctg
     6541 accctgcaga cggctgccgg cgccgctagc cagacattgt tcggactgct cggcgccgag
     6601 gatctggcct acctggccgt tcccctgacg gatcgcaagc aggcccgcct ggggctgctg
     6661 gtgctgtggt tcgatgcgcc gccggaccgg gacctggtgc atttcgtcga agctctgtcc
     6721 ggttcggcgg ccatgtcggt cgagacgcgg gccctgatcc gcgcccagaa ggagctgttc
     6781 gaggccttca tccagctcat cgccggcgcc attgatgcga agagtcccta caccggcggt
     6841 cactgcgccc gcgtgccgga gctcaccaag atgcttgccc aggccgcctg taacagcgcg
     6901 gacggacctt ttcgcgattt ccggctgggc gaggaggact gggaggcggt gcatatcgcc
     6961 gcgtggctgc acgattgcgg caaggtcacc acccccgagt acgtggtgga caaggccacc
     7021 aagctggaga ccctgtacga ccgcatccac gagatccgca tgcgcttcga ggtgctcaag
     7081 cgtgacgcga ccatcgccta ctggcaggcg cgcgcggaag gtgccgacga ggccgccgcc
     7141 aagggtcggc tggtggagac ccacgcggcg ctggaccggg agttcgcctt cgttgcggcc
     7201 tgcaacgagg gggcggagtc catggcaccg gaagacgtgg cgcgcctgca gcagatcgcc
     7261 cggcgcacct ggcagcgcac cctggacgat cgcctcggcc tgtcgccgga agagcggcgc
     7321 cgccgcgccg atgcggggcc cgtgcgcctg ccggtcaccg agccactgct ggccgacaag
     7381 cccgagcacc gctatgaacg gggtgcgatg gacctgctgc cggcggacaa cccgtggggc
     7441 ttccgcctca aggtaccggc gctgctctac aaccggggcg agctgaccaa tctctccatc
     7501 acccggggca ccctcacgga cgaggatcgc tacaaaatca acgagcacat cgtctacacc
     7561 atccgcatgc tctccgaatt gcccttcccg cggcatctgc gggccgtgcc ggagatcgct
     7621 ggcggccacc acgagaagat ggacggttcc ggctaccccc gtgggctgaa gggcggggag
     7681 atgagcctgg tggcgcggat gatggccatc gccgacatct tcgaggcgct gaccgcggtg
     7741 gatcgcccct acaagaccgg caagcgcctg tccgaggcgg tgagtatcat ggccgggatg
     7801 cgtgaccggg gacacatcga tccggatctc tttgccctgt tcctgcgctc gggcgtgtat
     7861 cgccgctacg ccgagcgctt catgcagcgc aagtgcgtgg acgaggtgga tgtggacgcg
     7921 atgctggccg aggtgaccgc ctagagcgtg tcgtgggtgc cgtcgcagaa cggcgcgcgg
     7981 cggctgtgct tgcagccgca gaggtaggcc agtccggagc gcaggggcgt gaaggtcttg
     8041 ggcgcgatgc cgctgcccgc gtggcggccg tcgcagaatg gctgggtcgc gctgcggccg
     8101 caggcgcacc aggtgtattc ccgcccggcc tcgacgggga cggtgtagga ggcgtactgg
     8161 gcgatctgcg gttcggtcat ggggaggctt ccgtgcaatc tccggggcgc ggcgcacccc
     8221 ggagccacga gcttacccga ttacggtttg taggcttcga cggcgatggt cagggtgaca
     8281 tcgtcgccca cgtagggggc gtacttgccg gcgttgaact cgctgcgctt gatcttggcg
     8341 acggcattgg cgccgcaggc atccttcttc agcatcgggt gaggcatgca gtggaaggag
     8401 gtgacggtca gggtgacggg cttggtgacg cccttgacgg tcaggttgcc ctccaccgac
     8461 gcgaccttgt caccgtcgaa gttcaccttg gtggacttgt aggtgatggt cgggtacttg
     8521 gcggtgtcga agaagtcttc gccctggatg tgctggttca gcagggggaa gccggtatcc
     8581 acggaggtgg catcgacggt gacgtccacg gtgccggact tggcggcgcg atccaggacg
     8641 atggtgccgg tggtcttgtc gaagcgggac agctgggtgg agtagccgaa gtggctgtat
     8701 tcgaagcgag ggaaggtgtg gctgccgtcg atggtgtagc tttccggcgc ggcgagggcg
     8761 gcggtggaca ggccggcggc caggatcagg gcggtgagct ttttcatgct gtgttctccg
     8821 ttgtgacagg ttgagggttt tgggttgggg tctgggctgt cgcgggggcg aacggcctta
     8881 cttctttgtg ggcgcagggg tggcgaccac gtggaacttg atctggatct cgttggcgac
     8941 ggcgctcacg tcggaccagg ggccttcacc gatgccgaag tccatgcgct tgagcacgaa
     9001 accgccgtcg aagacgccgt tggcgccgtc ctgcctgaac gtgaagggcg ccaccacgtc
     9061 gtgggtctgg cccttgatgg tcatcttgcc gctcacttcg tagcggccgc cgcccacggc
     9121 cttgacggcg gtggacacga agcgggcggt ggggaaggcc ttcacgttga accactgctt
     9181 gccgacgact tcctcgttgg catccttgga gccggcgtcg atgctggcca ggtcgatctc
     9241 cagcacggtg ctggcggtcg cgggcttggc cgggtcgaag gcgagctggg cgctgaactt
     9301 gcggaacagg ccatccaccg gcacgcccat ctgcttggac gtgaacgcga ggctgctctt
     9361 gtcggtcagc acctgggtga agtccgccgc gtgggcggcg ccggtggtga gacccgccag
     9421 cagggcgagg gtcagggtgg ggcgcagggt cttcatggct tctctcgtgt gtcgggttcg
     9481 ggggtcagac gcggccgaag ggcagcatgc ggcgcagggt cgcgtccttg tcgatcaggt
     9541 ggtgcttgag ggcggcggcc acgtgcaggc cgaccagacc gaagagcagc cagttgaggg
     9601 attcgtggac ctcttcgagg atttcgccga gatccgggtt ctttgcgatc aggtcgggaa
     9661 tcggcagcac gcccaggtac accacctgaa agcccttggc cgagctcatc agccagccgg
     9721 tgaggggcac cagggccatc agcacgtaga ggagccggtg ggtgacctct gccgccgtct
     9781 gttgccaggc cggtgccggc agcggggcgg gcgggcgatg ggtgaggcgc cacagcaccc
     9841 gcaggacgaa caggcccagc accgtcatgc cgatccactt gtggtacgag tagagcttga
     9901 gcttggtggg cgagaggggc agctcgtgca tgtacaggcc cagcggaaag gcggcgaaga
     9961 tcagcagcgc gatcagccag tgggcggcga tggcggggct ggaatagcgt tcggtgttca
    10021 tggtcagggc tccggggtgt aagggatgat gctgtccttt tgccacaggt aggcatccat
    10081 ggccagaccg ccataggtga attccggttc cagacggagg gcgatttcgt ccgggtcggc
    10141 tgcaccgagg gtcacgaaca ggggcagcag gtgctcctca ctgggatggg cggcggcgcc
    10201 cagggggctc tggcggcggt attccagcag cgtggggcga tcgttgcggg cgacggccgc
    10261 accgacccag tcggcgaagg ccttggcctg gggcaggggc tcgccaccgc cggaccagtc
    10321 cagccagccg aagttgtggg tgatggcgcc tgaggcgagg atcagcacgc cctcgtcccg
    10381 caggccgcgc agggcggtgc cgatgcggcg gtgccagtcc ggcccggcgt ggggttgcag
    10441 ggcgagctgc acgacgggga tgtccgcggc cgggtacatg gccttgaggg gaatccacgc
    10501 gccgtgatcc aggccccggt cgggcacctc ggcacagggc aggccggccg ccaccagcgc
    10561 atcgcgcacc cgggcggcca cgtccggcgc tccgggtgcc gggtattcca tggcgtagag
    10621 cggttccggg aagccgccga agtcgtggat cgtggcgggc atggagaccg tgcttacggt
    10681 aggctggcgc gccgcccagt gggccgagac gacgaggatc gcccgtgggc gcggcaggcg
    10741 ccggcccaga tcggcccaca gggccacggt ggcagcgggc cgcagcagca cgtcgggtgc
    10801 gccgtgggag acgaacagca gggggtgacg ggcgctcatg atgcggtcct cggggcagtg
    10861 acaggggcga agtactcgcg gatcaggcag tccagggagc ggggcgggtg gcccaccagg
    10921 gcagcaaagt catgggtgac gcgggccagg cggccctcgg cgacggccgc gaagaggctg
    10981 gcgacgccct cggccagccc gtccggcagc ccggcggcac gcagcgtggc ggcctgttcg
    11041 gtgaccgtca cgggcaggta gcgcaggggc cggccgctga cccgggcgat gcgcgcggcc
    11101 agctccgcgt aggcgacggc ctcgctgccg gtcaattcat acacgcgctt gtccaggcgc
    11161 ggggcgaggg cggcggcggc cagggcgcgg gcgagatcgt cccgggagac caggctcacc
    11221 gcgccgttgc cggcggccag gcagaaggcg tctccgtccg ggccgggcac caccagcgcg
    11281 ggcagatcgt cggcgtagag cgggttgcgc aggatcgtcg catccagccc gctggcggcg
    11341 agggcgtctt cggtttggcg gtgcacgcgg gcgaactccg ccggcgaatc ggtgcccacg
    11401 tcgaggaagc tggtgtacag gacgcgctgc acgcccgccc tgcgggcggc cgagatggcg
    11461 gccgcatgtt gggcgatgcg ctgcgcggag gggccgtcgg tggagaccag caccagggtg
    11521 cgcacgccgg caaaggtggc ggcgagggtc tcgggctggt cgaagtcgcc ggcgcggacg
    11581 tccaccccgc gggcggccag atcggctgcc agcgccggct ggcgcacgct caccgccagc
    11641 gccggtgcgt cgcgcagcgg gcggcgtgcg atcaggtgct ggacgatgcg ccggccaaga
    11701 cggccgttgg cgccggtgac gaggatcacg gaggactcct gtggcgggtt gatgaactgg
    11761 gtgccactgt aggctttcaa ttgagacaga aatagccttt aatcgggaaa tctttattgc
    11821 tggatatgca atgatggatc ggcttcaggc gatggaggtc ttcgtccggg tggcggaggc
    11881 ggggagtttt gcagcggcgg cggaccagct cgatctggcc cgctccgtgg tgacgcgcct
    11941 ggtcgcggcg ctggaggcgc gtctgggcgt caagctcatc gcccgcagca cccgcagcct
    12001 gcggctcacc tccgcgggac aggcctacct ggagcgctgc cgcgagatcc tcgatctggt
    12061 gggcgtcgcg gaaggggaac tgggcggcaa cgaacgcgcc ccccgcgggc cgatccgcct
    12121 gagcgtaccg gtgagcttcg gcatccatca tctggcgccg ctggtcgcgg atttcgtcac
    12181 cacctggccg gacgtgagcc tggacatcga cttcaacgat cgccaggtga atctggtgga
    12241 ggcgggcgtg gacatggcga tccgcatcac gacccagctc gaccccagcg tggtggcgcg
    12301 caggctcacg gtgtcgcgca tgctcaccct ggccgcgccc gagtacctgg cccgccacgg
    12361 ccgtccggtg cgtcccgacg atctgctgga ccatccctgc ctgggctaca ccggtaccgc
    12421 gcgggccagc tggccctacc gcgtggcggg cacgctgcaa tgggtgccgg tgcgcggccg
    12481 cctgcagagc aacaatggcg acgccctgct ggacgccgcc cggcgcgggc tgggcatctg
    12541 cctgcagccc agcttcatcg cggcggcggc cgtgcaggcc ggactgctgg aagtgctgct
    12601 ggcggacttt cccggccctg aactgggcat ctacgcggtg ttccccagcc atcgctacgt
    12661 gcccagccgg gtgcgcctgc tggcggacta cctggccgca cgtatcggcc cggtacctta
    12721 ctgggagcag ggcctgccgg cgtgagccgc gcggtgcggg gcatgggcga tgcaaagacc
    12781 ctgctgtgcc gcgggttggc ggcgccggac gcctcgccta ccatgggcgc cccggggccc
    12841 gtgcccctgt tccgaacatc gatgagagag gagtccccca tgtccagtac cgtcaccctc
    12901 ggcggcaatc ccgtcaccgt cgccggcgat ctgccccgcg tcggcgccac ggcgcccgct
    12961 ttttccctgg tcgccaagga cctgtccgag gtcacgctgg cgaactttgc cggtcagcgc
    13021 aaggtgctca acatcttccc gagcatcgac acgcccacct gcgccacctc ggtgcgcaag
    13081 ttcaaccagt ccgcctccag cctgcccaac accgcagtgc tgtgcatctc cgccgacctg
    13141 cccttcgccc agaaccgctt ctgcggcgcc gaaggcctct ccaacgtggt gaccctgtcc
    13201 accctgcgcg ggcgggaatt cctcgaagcc tacggcgtcg ccctcgccag cggcccgctg
    13261 gccgggctgg cagcccgcgc ggtggtggtg gtggacgagc aggacgtggt gcgccacgtg
    13321 gagctggtgc ccgagatcaa gaacgagccg gactacgctg cggcgctggc ggcactcggc
    13381 tgattccgcc gggtgcggag cccggcgtga tccgggcggt gggtccgctg cgagcggcgc
    13441 gcaggtcggg tcggtatgca ggggctgcac gcgcagcccg ccgccaccgg attcgaccgc
    13501 aagaaacggg atgcgcaggg ccgtccggcc ccgtacagtg caggcatcgc gcccacgctc
    13561 gggccttgcg gagcacccca tgactgcctc cacgtcctcg ccacccactg ccgccgaccc
    13621 gcgctgggcc gccgtccagg cgcgggatgg cacggccgac gggcatttct ggtacgccgt
    13681 caccaccacc ggcgtgttct gtcggccgtc ctgcccgtcg cggcgcccgc gaccggagca
    13741 tgtgcgcttc ttcgactccg cggcggccgc ccgtgaggcg ggcttccgac cctgtctgcg
    13801 ctgccatccg gacaccctgg ccgaccccca tgtcgccctc atcgccacgg tgtgccgcgc
    13861 catccaagcc gccgagacgc cgcccagctt gcgggcattg tccgtgctcg ccggcctcag
    13921 cgccgggcat ctgcagcgcg tgttcaaggc cgccaccggc ctcagcccgc gcgcctacgc
    13981 cgcggccctg cgcgccggtc gtgtgcgcac agggctcgcg gcgggggcac cggtcaccca
    14041 tgcggtgctg gacgctggct tcggctccac cggccgcttc tatgcccgct ccgcccaggt
    14101 cctcggcatg gcgcccgccg cctggcgcgc gggcggtgcc ggtgaggaca tccgtttcgc
    14161 tgtgggggcc tgcgagctgg gggcgttgct ggtggccgcc agcccgcgcg gcatctgcgc
    14221 cctgagcctc ggtgacgacc cggatgcgct ggtgcgtgcc ctgcaggacc gctttccccg
    14281 cgcctgcctg gtgggtgacg acccggcctt cgccacctgg gtgagccgag tcgccggcta
    14341 cctgcaggcg ccgcgggatg ggctggagct gccgctggat ctgcgcggca cggccttcca
    14401 gctgcgcgtc tggcacgccc tgcagggcat cccaccgggc gccacgatca gctacgccga
    14461 gctggcccgc cgcgtcggcg caccccgtgc ggtgcgagcc gtggcgggcg cctgcgcggc
    14521 caatccggtg gcgctggcgg tgccctgcca tcgggtggtg cgcagcgacg gcagcctgtc
    14581 cggctatcgc tggggcgtgg aacgtaaggc ggccctgctg acgcgggagg cggagcgcgg
    14641 cggccgtgac gacgcctgat ctcttcgaag gctggacgcc ggaggacgtc ggcccggagg
    14701 cgctgagccc cggcgcggtg cacctgcgcg gatttgtccg cgccgaggcc gccgcggtgc
    14761 tcgccgaact ggaccgcgtc cttgcccacg cgcccctgcg tacgatgcag acgcccggcg
    14821 gccggtcgat gtccgtggcc accagcaact gcggcgcgcg gggctgggtc agtgacgccg
    14881 ccggctaccg ctatgcgcct gccgatcccc tcggcggcgc agcgtggccc accatgccgc
    14941 ggctgtggcg caccctcgcc gagcgggccg cagccgcggc cggctttggc ggcttcacgc
    15001 ccgatgcctg cctcatcaac cgttacgcgc cgggtgcccg catggggctg caccaggacc
    15061 gggatgaggc ggacttcacc tggccgatcg tctccttctc cttcggtctg ccggcgatct
    15121 tcctcttcgg ggggctgcgc cgaggcgacc ccgcgcggag gatttccctg ttccacggcg
    15181 acgccgtcgt ctggggcggc ccgtcgcgcc tggcctttca tggcgtggcg ccggtgaagc
    15241 cgggcgagca tccgctgacc ggcgcgtgcc ggatcaacct gaccttccgg cgggcgtgag
    15301 ggcgggccca ccggaaagca cacggccagc ctcggtgagg ctggccgtgt gcgtgaggat
    15361 gctggtgggc ccagcaggac ttgaacccgc gaccaagcga ttatgagtcg cctgctctaa
    15421 ccgactgagc tatgggcccg gaaaagggga gggggaccgc aggcttcgcg gcggtccggt
    15481 gaagctcggc atgctaccag actatgccga cctgcaccgg cggccggtgg gtgcgtggcc
    15541 gcaccggccg gacgggatca ggcttcgttg tcgaggaagc tgcgcaggcg ctcggagcgg
    15601 ctggggtggc gcagcttgcg cagggccttg gcttcgatct ggcggatgcg ctcgcgggtg
    15661 acgtcgaact gcttgccgac ttcctccagg gtgtggtcgg tgttcatctc gatgccgaag
    15721 cgcatgcgca gtaccttggc ttcccgcggg gtgagggagt cgaggacttc cttggtggcg
    15781 tcgcgcaggc tggagtacag cgcggcgtcg gccggggcca tggtggcgtt gtcctcgatg
    15841 aagtcgccca g
//
LOCUS       DAOQPA010000086        15438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1933_length_15438_cov_18.604368, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000086 DAOQPA010000000
VERSION     DAOQPA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_15438_cov_18.604368"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKX01_12060"
     CDS             complement(<1..370)
                     /locus_tag="PKX01_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76908.1"
                     /translation="MNELALAIPFLGRRHPAAARAVVLLLALAAPLIAILFGKTWVRV
                     LDFAMLYVMLAIGLNLVVGFAGLLDLGYIAFYAVGAYTFAFLASPHFELHLPFWLVLP
                     LGAAFAALAGIILGLPTLRLR"
     gene            complement(375..1307)
                     /locus_tag="PKX01_12065"
     CDS             complement(375..1307)
                     /locus_tag="PKX01_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMZ76909.1"
                     /translation="METLLQQVVNGLVVGSVYALIALGYTMVYGILGLINFAHGDVLM
                     VGALVALEAIELIQARWPDLGAIPTLLAGLGVATATCMALGYTIERTAYRRLRNAPRL
                     APLITAIGMSFLLQTVAMIVWGRNYHTFPQLVSTTPLEPVSGVFITPVQIVILATSAL
                     LMVALVLLVNKTRLGRAMRATAENHRVASLMGVDTNKIIASTFIIGSGLAAVAGVMFS
                     SNYGIAHYAMGFMPGLKAFTAAVLGGIGNLGGAMLGGIVLGLVESIGAGYIEDMSFGF
                     LNSSYQDIFAFAILGIVLIFRPTGLLGERVADRA"
     gene            complement(1399..2511)
                     /locus_tag="PKX01_12070"
     CDS             complement(1399..2511)
                     /locus_tag="PKX01_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ76910.1"
                     /translation="MKHTLVALAVLGLGASAVHAQEVVKLGTAAPLTGTQAHLGKDIE
                     NGVRLAVEEYNAKGVTLGGKKVKFEVQAEDDQADPRAATTVAQRLVDSGVKGVVGHLN
                     SGASIPASRIYNDAGLPQISPASTNPKLTQQNFKAVFRTIANDVQQGLAIGKYAATKL
                     GKKIALIDDRTAYGQGLADEVEKAVKASGGQIVGREFTSDKANDFQAILTRIKGQNPD
                     VIVYSGMDTQGGPMLKQIKQLGINAKFITGDGGCTGEIIKLAGDAIGANAYCTQPGLP
                     LEQMPGGKDFNARFKKRFNADVQIYAPYAYDATSAIIEAMKAANSADPARYAPAIAKV
                     NFPGVIGKVAFDAKGDILDGAVTVYQFKGGKWEPTR"
     gene            complement(2568..3683)
                     /locus_tag="PKX01_12075"
     CDS             complement(2568..3683)
                     /locus_tag="PKX01_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ76911.1"
                     /translation="MKRLLTCCVLAAALAGPARADTVVKIGFAGPLTGPIAHVGKDEQ
                     FGAQLALDDVNTKGLTLNGQKVKFELVPEDDQADPRVATTVAQRLADAGVKGVVGHVT
                     SGAAIPASRIYEQAGIAAITPSSTTPKLTQQGFRSIYRVIANDLQQGAAMARFATGVL
                     KAQRIAIIDDRTAYGQGLADALVESLKGLGVTPVGREFTHDKATDFTAILTRLKATRP
                     DAVFYGGMDAQGAPLLKQMQQLGLKVRFLGGDGVCTAEMIRIAGPALSGDVYCTQAGL
                     AMDRMPGGKDFGARFRKRFNADVQLYAPYSYDATMALVEAMKLANSAEPAKYLPLMQK
                     VSFKGVTGPIAFDARGDSREGGVSLYQFNAGRWESRN"
     gene            3813..4295
                     /gene="bamE"
                     /locus_tag="PKX01_12080"
     CDS             3813..4295
                     /gene="bamE"
                     /locus_tag="PKX01_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HMZ76912.1"
                     /translation="MRSLVTLLCSAAAALGLGACDAVTLKDLKPGVSTGYDVRDRLGT
                     PGIEWRNADGSVTWEYSRQPEGTECFMATIGPDNVLRSLENVLTPQNFARVQAGWDKD
                     QVRRLLGKPRTVQTFALKQEEVWDWRLPPEVSADVFFNVHFDRDGRVTGTSRSVQPRG
                     "
     gene            complement(4345..4872)
                     /locus_tag="PKX01_12085"
     CDS             complement(4345..4872)
                     /locus_tag="PKX01_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76913.1"
                     /translation="MNASTPVVPEPHVSDATPGEADNPTLRRYRRMLSLLLARYTLRH
                     LVRLYAAFEGDLLLPIVLGEVGLYNVSGMDVDEAARGQFRCGCDEDFRKLVLRPCNAF
                     SISSSTGIPRETVRRKVARLAELGWIVRDSRGGLTITAKAAAECLQPFDRENLADFLN
                     TAERLRTLLTAEIKA"
     gene            complement(4994..6184)
                     /locus_tag="PKX01_12090"
     CDS             complement(4994..6184)
                     /locus_tag="PKX01_12090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ76914.1"
                     /translation="MADLILLPGKERSVLRRHPWIFAGSVARLEGRARPGDTVIVKDA
                     QGTPLARAAWSPSSQIRARVWSFDPDLSVDHAWFKRAVAASVARRAAHPWLAGQDGVR
                     LIHGESDGLPGVIADRYGEVVVVQLTSAGSDKWRDAIVAGLAQATGCRAVYERSDSDV
                     RKLEGLEPVTGLVHGALPDAPLTIVEHGVRMEVDVAAGHKTGFYLDQRDNRRLTGQLA
                     ADRDVLNCFCYTGGFSLQALAGGARSVLSIDSSGPALATAARNVALNPQLDAARAEWR
                     EDDVFQALRALRAEGRRFDLIILDPPKFAPSAAHAERAARAYKDINLLGLRLLKEGGI
                     LMTYSCSGGIGLELFQKIVAGAAADVGVDARILHRLCAAPDHPVGLAVPEGEYLKGLA
                     CQVS"
     gene            complement(6432..7082)
                     /locus_tag="PKX01_12095"
     CDS             complement(6432..7082)
                     /locus_tag="PKX01_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HMZ76915.1"
                     /translation="MNASDVVQFLKDHPGFFDDNPSLLTELTFTHPHAGQAISLTERQ
                     LLALRERIAQLEDKFAELLEFGEENDGISEKVHRLTLSLLEAEDFESIRQAIYTHLLE
                     DFSVPHVAFRVWNSVLSRSSPEFSPVSEAVRFFTGDLGDPYCGPPNQDEVIAWFGPAA
                     RHIRSVALVPLARDDQPFGLLAMGSEDSERFYPEMGTLYVTRIGEMVACALRRQLG"
     gene            complement(7116..7955)
                     /gene="dapF"
                     /locus_tag="PKX01_12100"
     CDS             complement(7116..7955)
                     /gene="dapF"
                     /locus_tag="PKX01_12100"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMZ76916.1"
                     /translation="MKQTLAFTKMQGLGNDFVMLDAVRQPVDLSPERVRALADRHFGI
                     GCDQLLVVEPSDTPGVDFRYRIFNADGGEVEQCGNGARCFARFVFEQGLTDKREIRVE
                     TAKGLITPRLEDDGTVTVDMGVPVLTPAEVPFVSDSDAYVQPLDVAGRTVAITAVSMG
                     NPHAVQVVADVDTAPVAETGPLIEHHPRFPARVNAGFLQVVDAHRVRLRVFERGAGET
                     LACGTGACAAVVAGILRELVVSPVTVETRGGELTIAWDGVGTPVMMTGPAVTVFSGEI
                     TLP"
     gene            complement(7952..8365)
                     /locus_tag="PKX01_12105"
     CDS             complement(7952..8365)
                     /locus_tag="PKX01_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMZ76917.1"
                     /translation="MFTNPSPDQLRTLLREVKSIAVVGLSPRPDRPSHRVARALQGFG
                     YRIVPVRPAVAEVLGEKAYARLADIPHPVDLVDVFRASDEVAPLVDECIALGVKRLWL
                     QDGVIDEAAAEKAQAAGMTVVMDRCVLRDYVDLIP"
     gene            8467..10296
                     /gene="sppA"
                     /locus_tag="PKX01_12110"
     CDS             8467..10296
                     /gene="sppA"
                     /locus_tag="PKX01_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ76918.1"
                     /translation="MERKSRPGLIRRMLTGLWRGVDRLRQISLNLLFIAVAVFLIAGW
                     WASRPTPLEAGSALLVAPVGQIVEQRTSRSPMSVLQGGDGIHQVQLRDIVDGIRAAAA
                     DSRIKALVIEPDGINTVGLSKLQEIAAAIAEFRKAGKKVYVHAKHYSQSQYYLAAQAD
                     EAFMNPDGYVFLSGFARYPTYYKGLLDSLGVRMQIFRVGTYKSFVEPYTRSDMSPEDR
                     EASRGYLEAAWQIFRQDIAAARPKAADKLDAYINDANAQMAAAGGDAARMARDAGLLD
                     GLKTADEWRDFLKARVGTDGDGKQYRHVDLAAYLAHVREAAPHPDERVGVVVAQGAIV
                     DGDQPAGVVGGDTAALLIRQARENDAIKALVLRVDSPGGSAFASEVIRRELELTRKAG
                     KPVIVSMSSVAASGGYWISTAADEVWASPATLTGSIGIFAMVPDLSGPMNKLGLTVDG
                     FGTTPLAGGLDPRRPLDPKVADLLQQSIDHGYKRFLQVVAQGRRMTPEAVDPIAQGRV
                     WLGTQAKEKGLVDKLGGLDEAVKAAAARAGLTRYDVTYVEKSLSPRDQLLTRLLDSEE
                     ESRASAAASPVDVLLANLRGELANLAHWNDPGFAYLHCLCVAP"
     gene            complement(10386..10724)
                     /locus_tag="PKX01_12115"
     CDS             complement(10386..10724)
                     /locus_tag="PKX01_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HMZ76919.1"
                     /translation="MPRIPHFAALMLAVTAGAWAQQPPRLEPLPEPPPPPPGMELDPS
                     LEPQVTIVKKGEDTVEEYRVNGQLYMIKVTPVNGPAYYLYDDVGNGDFLRRDSQDSGV
                     RVPKWVIKRW"
     gene            complement(10770..11495)
                     /locus_tag="PKX01_12120"
     CDS             complement(10770..11495)
                     /locus_tag="PKX01_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ76920.1"
                     /translation="MTAKDKLPKMAPGSESQHFTARESWRIFGIMAEFVEATERLKPI
                     RPAVSIFGSARVKPDHPYYAQTERIARLLSDAGFSVISGGGPGIMEAANKGAYFGKSP
                     SVGLNIQLPMEQQANPYQDISQTFQHFFARKFMFVKFASAYVVMPGGFGTLDELLEAM
                     TLIQTGKSRKIPVILVHEPFWRGLVDWFKDRLVSEEMIHPEDLNLIQVIDEPEKVVDA
                     IFNHYQTRGFLPLPDEHELMLNL"
     gene            11552..14284
                     /gene="polA"
                     /locus_tag="PKX01_12125"
     CDS             11552..14284
                     /gene="polA"
                     /locus_tag="PKX01_12125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMZ76921.1"
                     /translation="MPTLLLVDGSSYLYRAFHALPDLRNSAGEPTGAVRGVLAMLRRL
                     ESDYKADYRACVFDAKGKTFRDDWFPAYKAQRPPMPEDLRAQIEPLHAAVRAEGWPLL
                     MEDGVEADDVIGTLTRQAAEAGWEVVISTGDKDLTQLVGPGVRWVNTMSEEVLDAAGV
                     EAKFGVPPGRIVDYLALIGDTVDNVPGVEKCGPKTAVKWLSQYGDLDTLVAHAAEVGG
                     KVGENLRKHLDFLPLGRKLVTVVTDLPLPVGPADLAVRPADRAALAELYRRMEFKSWL
                     KELESAPPAGASETPPAGDAPPPSAEPAAPGAHRQGYATLLDWDAFDAWLAQIQAAEL
                     TAFDTETTGLDPLVATLVGMSFATVTGQAAYLPLAHTYADAPAQLPLAEVLARLKPWL
                     ESDAHRKLGQNLKYDAHILANHGIRLAGIAEDTLLESYVLESDKSHDMDSLASRHLGL
                     KTLTYAEVCGKGAKQIGFGEVDVARATEYAAEDADITLRLHYALSAQLAAEPALAALY
                     RDVELPTARVLFEMERTGVLIDDFLLAQHSEELGRRLHALELDAHALAGQPFNLGSPK
                     QLGEILFTKLGLPVVKKTATGQPSTDEDVLTQLAEDYPLPKLLLEHRSLAKLKNTYTD
                     KLPLMVNPRTGRVHTSFSQAVAVTGRLASTEPNLQNIPIRTPEGRRIRAAFIAPAGHR
                     IVSADYSQIELRIMAHLSGDAGLLNAFAHGEDVHRATAAEIFGITPMEVGSEQRRTAK
                     VINFGLIYGMSAHGLARNLGIERAAAATYIDRYFARYPGVAAYMERTRTEARTQGYVE
                     TVFGRRLYLPEIRAQQVGRRQAAERAAINAPMQGTAADLIKKAMIAVSAWLKTAGVRA
                     RLILQVHDELVLEVPDDEVALIRERLPQLMAGVAQLSVPLVAEVGVGANWDEAH"
     gene            14371..14901
                     /locus_tag="PKX01_12130"
     CDS             14371..14901
                     /locus_tag="PKX01_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HMZ76922.1"
                     /translation="MQHHRSARLPVLFLLSCAALALGACSSAPKKDLTYRSERFNTNS
                     PYEVAVDVAPTAACDAGRRALLSQGYVIEDAKPDAVKARKFFQPERNVHVQINFVLSC
                     LADGDGTEVFANAREVRDELKASSSSAGVSVAGIGSLNLPLGGNGESLVKVGEETITD
                     SDFYGRFFALMQTYIR"
     gene            14901..>15438
                     /locus_tag="PKX01_12135"
     CDS             14901..>15438
                     /locus_tag="PKX01_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HMZ76923.1"
                     /translation="MTLRSTLAAGVAALVLGVPVARAADVAQFAPCVAGLKAGAVARG
                     VSPAGYERLTAGLEPDLKVLDFLDYQPEFRTPIWDYLSGLVDDERVADGQAMLKQWGD
                     TLAAVAARYHVDPATVVAVWGVESNFGRNFGTRPLLTSLSTLSCYGRRQAYFRGEFYD
                     ALKIVDGGDVKAEQLTGSW"
BASE COUNT         2462 a         5229 c         5398 g         2349 t
ORIGIN      
        1 cgcgcaggcg cagggtgggc aggccgagga tgatgccggc cagggcggcg aaggcggcgc
       61 ccagcggcag caccagccag aacggcaggt gcagctcaaa gtggggcgag gcgaggaagg
      121 cgaaggtgta ggcgcccacc gcgtagaagg cgatgtagcc caggtcgagc aggccggcaa
      181 agcccaccac caggttgagg ccgatggcga gcatcacgta gagcatcgcg aaatcgagga
      241 cgcgcaccca ggtcttgccg aacaggatgg cgatgagcgg ggcggccagg gccagcagga
      301 gcaccaccgc gcgggcggcg gcgggatggc ggcggccgag gaagggaatg gcgagggcga
      361 gttcgttcat ggcatcaggc ccggtcggcg acgcgttcgc ccaggaggcc ggtcggccgg
      421 aagatcagca cgatgccgag gatggcgaag gcgaagatgt cctggtagct ggagttgagg
      481 aagccgaagc tcatgtcctc gatgtagccg gcgccgatgg attccaccag gcccaggacg
      541 atgccgccga gcatggcgcc gcccaggttg ccgatgcccc ccagcactgc ggcagtgaag
      601 gccttgaggc cgggcatgaa gcccatggcg taatgggcga tgccgtagtt ggacgagaac
      661 atcacccccg ccacggcggc caggccggaa ccgatgatga aggtggaggc gatgatcttg
      721 ttggtatcca cgcccatcag gcttgcgacc cggtggttct cggcggtggc acgcatggcg
      781 cggccgaggc gggtcttgtt caccagcagc accagggcca ccatcagcag cgccgaggtg
      841 gccaggatga cgatctgcac gggcgtgatg aagacgccgc tgacgggttc cagcggtgtg
      901 gtggagacca gctgcgggaa ggtgtggtag ttgcggcccc agacgatcat ggcgacggtc
      961 tggagcagga aggacatgcc gatggcggtg atcagcggcg ccagccgcgg cgcattgcgc
     1021 aggcggcggt aggcagtgcg ctcgatggtg tagccgaggg ccatgcaggt ggcggtggcc
     1081 acgccgaggc cggcgagcag cgtggggatc gcgcccaggt ccggccagcg ggcctggatc
     1141 agttcgatgg cctccagcgc caccagggcg ccgaccatca gcacgtcgcc gtgggcgaag
     1201 ttgatcaggc cgaggatgcc gtagaccatg gtgtagccga gggcgatgag ggcgtacacg
     1261 ctgcccacca ccagtccatt gaccacctgt tgcagaaggg tttccatcga agcgacccgg
     1321 aacgggaagg gaaggccggc gcgggccggt ccgggcgggg cgcaccggcc ggcgcacccc
     1381 gcggtcggaa gactcagatc agcgggtggg ttcccacttg ccgcccttga actggtagac
     1441 cgtgacggca ccgtccagga tgtcgccctt ggcgtcgaag gcgaccttgc cgatcacgcc
     1501 ggggaagttc accttggcaa tggccggcgc atacctggcc ggatcggccg agttggcagc
     1561 cttcatggct tcgatgatgg ccgaggtggc gtcgtaggcg tagggagcgt agatctgcac
     1621 gtcggcgttg aagcgcttct tgaagcgggc gttgaagtcc ttgccgccgg gcatctgctc
     1681 caggggcagg ccgggctggg tgcagtaggc gttggcgccg atggcgtcgc ccgccagctt
     1741 gatgatctcg ccggtgcagc cgccgtcgcc ggtgatgaac ttggcgttga tgcccagctg
     1801 cttgatctgc ttgagcatgg ggccgccctg ggtgtccatg ccgctataaa cgatcacgtc
     1861 cgggttctgg cccttgatgc gggtcaggat ggcctggaag tcgttggcct tgtcgctggt
     1921 gaattcgcgg ccgacgatct ggccgccgct ggccttgacg gccttctcca cctcgtccgc
     1981 caggccctgg ccgtaggccg tgcggtcgtc gatgagggcg atcttcttgc ccagcttggt
     2041 ggcggcgtac ttgccgatgg cgaggccctg ctgcacgtcg ttggcgatgg tgcggaagac
     2101 cgccttgaag ttctgctggg tgagcttggg attggtggag gcgggggaga tctggggcag
     2161 tccggcgtcg ttgtagatgc gcgaggcggg aatcgaggcg ccggagttga ggtgcccgac
     2221 gacgcccttc acgccgctgt ccacaaggcg ctgggccacg gtggtggcgg cgcgggggtc
     2281 ggcctggtca tcctcggcct ggacttcgaa cttgaccttc ttgccgccca gggtcacgcc
     2341 cttggcgttg tattcctcga cggccagccg cacaccgttc tcgatgtcct tgcccaggtg
     2401 ggcctgcgtg ccggtgaggg gcgcggcggt accgagcttg acgacttcct gggcgtggac
     2461 ggccgatgca ccaagaccga gcacggcgag tgcgacgagg gtgtgtttca tgggcgattc
     2521 tcctgtggat ggggacggca aggacggtca agcaatatcg gggccggtca gttgcgggat
     2581 tcccacctgc cggcgttgaa ctggtagagg ctcacgcccc cttcgcggct gtcgccgcgg
     2641 gcatcgaagg cgatcgggcc ggtgacgccc ttgaaggaca ccttctgcat caggggcaga
     2701 tatttggccg gctcggccga gttggccagc ttcatcgcct cgacgagggc catcgtcgca
     2761 tcgtagctgt aaggcgcgta gagctgcacg tcggcgttga agcgcttcct gaagcgggcg
     2821 ccgaagtcct tgccgccggg catgcggtcc atggccaggc cggcctgggt gcaatacacg
     2881 tcgccgctca gggccgggcc ggcgatgcgg atcatctcgg cggtgcacac gccgtcgccg
     2941 ccgaggaagc gcaccttgag gccgagctgt tgcatctgct tgaggagcgg cgcgccctgg
     3001 gcgtccatgc cgccgtagaa caccgcgtcg gggcgggtgg ccttcagcct ggtgaggatg
     3061 gcggtgaagt cggtggcctt gtcgtgggtg aattcccgcc cgaccggcgt cacgccgagg
     3121 cctttgaggc tctcgaccag cgcgtcggcg agaccctggc cataggccgt gcggtcgtcg
     3181 atgatggcga tgcgctgggc cttgagcacg ccggtggcga accgtgccat ggcggcgccc
     3241 tgctgcaggt cgttggcgat gacccggtag atgctgcgga agccctgctg ggtgagcttg
     3301 ggcgtggtgg acgacggggt gatggcggca atcccggcct gctcgtagat gcgcgaggcg
     3361 ggaatcgccg cgccagaagt gacgtgcccg accacgccct tcacgccggc gtcggcgagg
     3421 cgctgggcca ccgtggtggc gacacggggg tcggcctggt cgtcctcggg caccagctcg
     3481 aacttcacct tctgcccgtt gagcgtgagg cctttggtgt tgacgtcatc gagggcgagc
     3541 tgggcgccga actgttcgtc cttgcccacg tgggcgatgg gcccggtgag ggggccggcg
     3601 aagccgatct tcaccaccgt gtccgcgcgg gcggggcccg cgagggcagc ggccaggacg
     3661 cagcaggtga gcaggcgttt catcggggtc ggttctccgc aaggggcatt cgtgccagaa
     3721 tcggcccgga ccttgcccgg accgccgcag ccgggaattg taaccaagcc tgccgggttt
     3781 gaacggccgg cgtcggaaag gaagtgctcg cgatgcgttc cctcgtcacc ctgctgtgca
     3841 gcgcagccgc cgccctcggc ctgggggcct gcgacgcggt caccctgaag gacctcaagc
     3901 ccggcgtgtc caccggctac gacgtgcgcg accgccttgg tacgccgggc atcgaatggc
     3961 gcaacgcgga cggcagcgtg acctgggaat acagccgtca gcccgagggc accgaatgct
     4021 tcatggccac catcgggccg gacaatgtgc tgcgcagcct cgagaacgtc ctcacgccgc
     4081 agaacttcgc ccgcgtgcag gcgggttggg acaaggatca ggtgcgccgt ctgctgggca
     4141 agccgcggac cgtgcagacc tttgcgctca agcaggaaga ggtgtgggac tggcgtctgc
     4201 cgccggaggt gagcgcggac gtgttcttca atgtgcactt cgaccgggac ggacgcgtca
     4261 ccggaaccag ccgcagcgtg cagccccgcg gttgagcaag ccgcgccccg ggtccgcacg
     4321 cggggccgtc atccggaaag gcgttcaggc cttgatttcg gccgtgagca gcgtgcgcag
     4381 gcgctcggcg gtgttcagga agtcggcgag attttcccgg tcgaagggtt gcaggcattc
     4441 cgccgcggcc ttggccgtga tggtcaggcc gccccggctg tcgcgcacga tccagcccag
     4501 ttccgccagg cgcgccacct tgcggcggac ggtctcgcgc gggatgccgg tggaggaact
     4561 gatggagaac gcattgcagg gccgcaacac gagcttgcgg aagtcctcgt cgcagccaca
     4621 gcggaactgg ccgcgcgccg cttcgtccac gtccatgccg ctgacgttgt agagacccac
     4681 ttcacccagc acgatgggca gcagcaggtc gccctcgaag gctgcgtaga ggcgcaccag
     4741 atggcgcagg gtgtagcgcg ccaggagcag cgacagcatc cgccggtagc gacgcagggt
     4801 ggggttgtcg gcctcgccgg gtgtcgcatc ggaaacatgc ggttcgggca ctacgggcgt
     4861 cgaggcattc atggagctct ccgaaaggga ccaccggtac ggtgtgccct gtgcggcgct
     4921 cccggcgaca agccgcgcag gatacacacc cgcgccgcgg ctcaaacccc cggactaccc
     4981 gctttgggca ctctcatgac acctggcagg ccagcccttt cagatattca ccttccggca
     5041 ctgcgaggcc gacggggtga tccggtgccg cgcagagccg gtgcaggatc cgcgcatcca
     5101 cccccacgtc ggccgcagct ccggccacga tcttctggaa cagctccagc ccgatgcctc
     5161 cggagcaaga gtaggtcatc aggattcccc cttctttgag caaacgcaaa ccgaggagat
     5221 tgatgtcctt gtaggcccgc gcggcgcgct ccgcgtgggc ggcggagggg gcgaacttgg
     5281 gcgggtcaag gatgatcagg tcgaaacggc gtccctcggc gcgcagtgcg cggagcgcct
     5341 ggaagacgtc gtcctcgcgc cattcggcgc gggcggcgtc gagctgcggg ttgagcgcca
     5401 cattgcgcgc cgcggtggcc agcgccggcc cggaggagtc gatggacagc acgctgcggg
     5461 cgccgccagc cagggcctgc agggaaaagc cgcccgtgta gcagaagcag ttgagcacgt
     5521 cccggtcggc ggcaagctgg ccggtgaggc ggcggttgtc gcgctgatcc aggtagaagc
     5581 cggtcttgtg gcccgcggcc acgtccacct ccatgcgcac gccgtgctcg acgatggtga
     5641 gcggcgcatc cggcagcgcg ccatgcacca ggccggtgac cggctccagg ccttcgagct
     5701 tgcgcacgtc cgagtcggag cgctcgtaga ccgcgcggca gccggtggcc tgggccagcc
     5761 cggcgacgat cgcatcgcgc catttgtccg aaccggcgct ggtgagctgg accaccacca
     5821 cctcgccgta gcggtcggcg atgacgccgg gcaggccgtc ggattcgccg tggatcaggc
     5881 gcacgccgtc ctgccccgcc agccacggat gcgccgcccg ccgcgccacc gaggccgcga
     5941 ccgcccgctt gaaccaggcg tgatccaccg acaggtccgg atcgaagctc cacacccgtg
     6001 cccggatctg ggacgacggc gaccaggccg cccgcgccag cggcgtgccc tgggcatcct
     6061 tgacgatcac cgtgtcgccc ggccgcgccc gcccctccag ccgcgccacc gagccggcga
     6121 agatccaggg gtgacgacgg agcacggaac gttccttgcc ggggaggaga atcaggtcag
     6181 ccatgacgaa gcaattcagt ggggttgagc ggcacgcggt tgggcgccgc gtcggaatcg
     6241 cgtgcgctga gcacgtgcaa tgagttcgga gtgaggaaag cgcgtccggg ccggatcgcc
     6301 cgaccccgcg tcagaggggt gcagaaagcg tcgcgagccg gtcgaggtcg tgacgagaag
     6361 cgcagccgta caagacgtac ggcgagcatc gcagtcgcga gatcggccgg cgcagtagct
     6421 ttatgcaccc ctcagccgag ctggcggcgg agggcgcagg ccaccatctc gccgatgcgg
     6481 gtgacgtaga gcgtgcccat ttcgggatag aagcgctcgg agtcctcgct gcccatggcc
     6541 agcaggccga agggctggtc gtcgcgggcg aggggcacca gggcgacgga gcggatgtgc
     6601 cgggccgcgg ggccgaacca ggcaatgacc tcgtcctgat tgggcgggcc gcaatacggg
     6661 tcgcccaggt cgccggtgaa gaagcgcacc gcttcgctga ccggcgagaa ttccgggctg
     6721 ctgcgggaca gcacgctgtt ccacacccgg aaggccacgt ggggcaccga gaaatcctcc
     6781 agcaggtggg tgtagatggc ctggcggatg gattcgaagt cctcggcttc cagcagggac
     6841 agggtcaggc ggtgcacctt ctcgctgatg ccgtcgttct cctcgccgaa ctccagcagc
     6901 tcggcgaact tgtcctccag ctgggcgatg cgctcccgca gggcgaggag ctgccgttcg
     6961 gtgagggaga tggcctggcc ggcgtggggg tgggtgaagg tgagctcggt cagcagcgac
     7021 gggttgtcgt cgaagaagcc gggatggtcc ttgaggaact ggacgacgtc ggatgcattc
     7081 atgggcgggc gccgggagag gagtgggtca cggggtcacg gaagggtgat ttcgccggag
     7141 aacacggtga cggccgggcc ggtcatcatg accggcgtgc cgacgccgtc ccaggcgatg
     7201 gtgagctcgc cgccgcgggt ctcgacggtg accggcgaga ccacgagttc gcgcaggatg
     7261 ccggccacca cggcggcaca ggcgcccgtg ccgcaggcga gggtctcgcc ggcaccgcgt
     7321 tcgaagacgc gcagccgcac gcggtgggcg tccaccacct gcaggaagcc cgcgttgacc
     7381 cgcgccggga agcgcggatg gtgctcgatg agggggccgg tctcggccac cggcgccgtg
     7441 tctacgtccg ccaccacctg cacggcgtgg ggattgccca tgctcacggc agtgatggcg
     7501 acggtccggc cggccacgtc gaggggctgg acgtaggcgt cggaatcgct gacgaagggc
     7561 acctcggccg gggtgagcac cggcacgccc atgtccacgg tgaccgtgcc gtcgtcctcc
     7621 aggcgcgggg tgatgaggcc cttggcggtc tcgacgcgga tctcgcgctt gtcggtgagg
     7681 ccctgttcga agacgaagcg ggcgaagcag cgggcgccgt tgccgcactg ttccacctcg
     7741 ccgccgtcgg cattgaagat gcggtagcgg aaatccacgc ccggcgtatc ggatggctcg
     7801 accaccagca actggtcgca gccgatgccg aaatgccggt cggccagcgc acgcacccgc
     7861 tcgggggaca ggtctacggg ctggcgcacg gcgtcgagca tgacgaagtc gttgcccagc
     7921 ccctgcattt tggtgaaggc gagagtctgc ttcatgggat aagatccacg tagtcccgca
     7981 gcacgcaacg gtccatcacc acggtcatgc ccgccgcctg tgccttttcg gccgccgctt
     8041 cgtcgatcac cccgtcctgc agccacagtc gcttgacacc cagggcgatg cattcgtcca
     8101 ccagcggtgc gacctcgtcc gacgcccgga agacgtccac gagatccacc gggtggggaa
     8161 tgtccgccag gcgggcgtag gctttttcac cgagcacttc ggcaacggca ggtcgaaccg
     8221 gtacgatgcg gtatccgaaa ccctgcaggg cgcgggcgac ccggtgactg gggcgatccg
     8281 ggcgcggcga caggccgacc acggcgatgg acttgacctc ccgcagcagt gtgcggagct
     8341 ggtcggggga tggattggta aacatggcgg gattctaccg gattgccgcg gcgtcgaaac
     8401 cttggacgcc gccaccggtc cccgcataac aggcccgcgc accgggccgc agcaacggag
     8461 agcacaatgg aacgcaagtc gaggcccggt ctgatccgca ggatgctgac cggcctgtgg
     8521 cggggggtgg atcgcctccg gcagatcagc ctgaacctgt tgttcatcgc agtcgcggtc
     8581 tttctgatcg ccggctggtg ggcgagccgc cccacgccgc tggaagcagg cagcgccctg
     8641 ctggtggcgc cggtgggcca gatcgtcgag cagcgcacct cgcgcagtcc catgagcgtg
     8701 ctccagggcg gcgacggcat ccaccaggtg cagctgcgcg acatcgtgga cggcatccgc
     8761 gccgcggcgg cagactcgcg catcaaggcg ctggtcatcg agccggacgg catcaatacc
     8821 gtcggcctgt ccaagctgca ggagatcgcc gcggccattg ccgaattccg caaggccggc
     8881 aagaaggtct atgttcatgc caagcattac agccagagcc agtattacct ggcggcccag
     8941 gcggacgagg ccttcatgaa cccggacggc tacgtcttcc tgtccggctt cgcgcgctat
     9001 cccacctact acaagggcct gctcgacagt ctgggggtgc gcatgcagat cttccgggtg
     9061 ggcacctaca agtccttcgt tgagccctac acccgcagcg acatgtcacc ggaagaccgg
     9121 gaagcgagcc gcggctacct ggaggcggcc tggcagatct tccgtcagga catcgcggcg
     9181 gcccgtccca aggccgccga caagctcgac gcctatataa acgacgccaa tgcgcagatg
     9241 gccgccgccg gcggcgacgc ggcccgcatg gcgcgggacg cgggcctgct ggacggcctc
     9301 aagacggccg acgagtggcg cgacttcctc aaggcgcgcg ttggcacgga cggcgacggc
     9361 aagcagtaca ggcacgtgga tctggcggcc tacctcgccc acgtgcgcga ggctgcgcct
     9421 caccccgacg agcgtgtcgg cgtggtggtg gcccagggcg ccatcgtgga cggcgatcag
     9481 cctgccggtg tggtgggcgg cgacaccgcg gccctgctga tccgtcaggc gcgggaaaac
     9541 gacgccatca aggccctggt gctgcgagtc gacagcccgg ggggcagcgc cttcgcctcc
     9601 gaggtgatcc gccgcgaact ggagctgacc cgcaaggccg gcaagccggt gattgtctcc
     9661 atgagctcgg tggcggcttc cggcggttac tggatcagca ccgccgccga cgaggtgtgg
     9721 gccagcccgg cgaccctgac cggctccatc ggcatcttcg cgatggtgcc ggacctctcc
     9781 gggccgatga acaagctggg cctcacggtg gacggtttcg gcaccacacc gctggcgggc
     9841 ggactggatc cgcgccggcc gctggacccg aaggtggcgg acctgctcca gcagtccatc
     9901 gaccacggtt acaagcgctt cctgcaggtg gtggcccagg gccgccggat gacgcccgag
     9961 gcggtggatc cgatcgccca ggggcgcgtg tggctgggga cgcaggccaa ggaaaagggt
    10021 ctggtggaca agctcggcgg gctcgacgaa gcggtcaagg ccgcggccgc ccgggccggc
    10081 ctcacccgat acgacgtgac ctacgtggag aaatccctgt cgccccgcga tcaactcctc
    10141 acccggctgc tcgacagcga ggaggaatcc cgcgcaagcg ctgctgcgtc accggtggat
    10201 gtcctgctgg ccaacctgcg gggcgaactg gcgaaccttg cccactggaa cgatccgggc
    10261 ttcgcctacc tgcactgcct gtgcgtggcg ccctgatccg gggcgccgcc gggcgttgag
    10321 gccgcccggc gcagcgcagt ccattgatcg gacccggctg cggccgggtc cggcgtttca
    10381 gggcctcacc agcgcttgat cacccacttg ggcacgcgca cgccggaatc ctggctgtcg
    10441 cggcgcagga agtcgccgtt gcccacgtcg tcgtagaggt agtaggccgg gccgttcacc
    10501 ggggtgacct tgatcatgta gagctggcca ttgacccggt actcctccac cgtgtcctcg
    10561 cccttcttga cgatggtgac ctggggctcc agggacgggt ccagttccat gccgggcggc
    10621 ggcgggggcg gttcgggcag gggttcgagc ctgggcggct gctgggccca ggcgccggcg
    10681 gtgaccgcca gcatcagagc ggcgaaatgc gggatgcggg gcatggtgtt ctcctcaaga
    10741 tgccggcatt gtaccccgcg ctccggcgct tacagattca gcatcagctc gtgttcgtcc
    10801 ggcaggggca ggaagccgcg ggtctggtag tggttgaaga tggcgtccac caccttctcc
    10861 ggctcgtcga tcacctggat cagattcaga tcctccgggt ggatcatctc ctcgctcacc
    10921 aggcggtcct tgaaccagtc caccagccca cgccagaagg gctcatgcac caggatcacc
    10981 gggatcttgc ggctcttgcc ggtctggatc agggtcatcg cctccagcag ctcgtccagg
    11041 gtgccgaaac cgcccggcat caccacgtag gccgacgcga acttcacgaa catgaacttg
    11101 cgcgcgaaga agtgctggaa ggtctgggaa atgtcctgat agggattcgc ctgctgctcc
    11161 atggggagct ggatgttgag ccccacgctg ggtgacttgc cgaaataggc gcccttgttg
    11221 gccgcctcca tgatgcccgg cccgccgccg gagatcaccg agaagcccgc gtccgacagc
    11281 agtcgcgcga tgcgttcggt ctgggcgtag tagggatggt cgggtttgac gcgggcgctg
    11341 ccgaagatcg acaccgccgg gcggatcggc ttgagccgtt cggtcgcctc gacgaactct
    11401 gccataatcc cgaaaatccg ccacgactcc cgggccgtga agtgttggga ttccgacccc
    11461 ggcgccatct tcgggagctt gtcttttgct gtcatttttc ttctgaggaa taggggccgc
    11521 cggcatcgtg ccgtcgggcc cgggcttgcc catgccgacc ctgctgctcg tcgacggttc
    11581 cagctacctg taccgcgcct tccacgccct gcccgacctg cgcaactcgg ccggcgaacc
    11641 cacgggcgcc gtgcgcgggg tgctcgccat gctacgtcgc ctcgaatccg actacaaggc
    11701 ggactaccgc gcctgcgtct tcgatgccaa gggcaagacc ttccgcgacg actggttccc
    11761 cgcctacaag gcccagcggc cgcccatgcc cgaggatctg cgcgcccaga tcgagcccct
    11821 ccacgccgcc gtgcgcgccg aaggctggcc gctgctgatg gaagacggcg tcgaggccga
    11881 cgacgtgatc ggcactctca cccgccaggc cgcggaggcc ggctgggagg tggtcatctc
    11941 caccggcgac aaggatctca cccagttggt gggaccgggc gtgcgctggg tgaacaccat
    12001 gagcgaggaa gttctcgacg cagccggcgt ggaggccaag ttcggcgtgc cgccgggccg
    12061 catcgtggat tacctggccc tcatcggcga caccgtggac aacgtgccgg gcgtcgagaa
    12121 atgcggcccc aagaccgccg tcaagtggct cagccagtac ggcgacctgg acaccctcgt
    12181 ggcccacgcc gccgaggtgg gcggcaaggt cggcgagaac ctgcgcaagc atctggactt
    12241 cctacccctc gggcgcaagc tcgtcaccgt cgtcaccgat ctgcccctgc ccgtcggccc
    12301 ggccgatctg gcggtgcgac cggccgaccg ggcggcgctg gccgagctct accggcgtat
    12361 ggagttcaag agctggctga aggagctcga atccgccccg cccgccggtg cctccgagac
    12421 gccccccgcc ggcgacgctc caccgccgtc cgccgagccg gccgcgcccg gtgcccaccg
    12481 tcagggctat gccaccctgc tcgactggga cgccttcgac gcctggctgg cacagatcca
    12541 ggcagccgaa ctgaccgcct tcgacaccga gaccaccggc ctcgacccgc tggtcgcgac
    12601 tctcgtgggc atgtccttcg ccaccgtcac gggccaggcc gcctacctgc cgctggcgca
    12661 cacctacgcc gacgcgccgg cccagctgcc cctggccgag gtgctggccc gcctcaagcc
    12721 ctggctggag tccgacgctc accgcaagct cggccagaac ctcaagtacg acgcccacat
    12781 tctcgccaac cacggcatcc ggctcgccgg catcgccgag gacaccctgc tggaatccta
    12841 cgtgctggaa agcgacaagt cccacgacat ggattccctc gccagtcgtc acctgggcct
    12901 caagaccctg acctacgccg aagtctgcgg caagggcgcc aagcagatcg gcttcggcga
    12961 ggtggacgtg gcccgcgcca ccgaatatgc cgccgaggac gcggacatca ccctgcgcct
    13021 gcactacgcc ctgtccgccc agctcgccgc cgagcccgcc ctcgccgccc tctaccggga
    13081 cgtggagctg cccaccgcgc gggtgctctt tgagatggag cgcaccggcg tgctcatcga
    13141 cgacttcctc ctcgcccagc attccgagga gctcgggcgt cgcctgcacg cgctggagct
    13201 cgacgcccac gccctcgccg gccagccctt caacctgggc tcgcccaagc agctcggcga
    13261 gatcctgttc accaagctgg gcctgccggt ggtcaagaag accgccaccg gccagccgtc
    13321 caccgacgag gacgtgctca cccagctcgc cgaggactac ccgctgccca agctgctgct
    13381 ggagcaccgc agcctggcca agctcaagaa cacctacacc gacaagctgc cgctgatggt
    13441 caacccgcgc accgggcgcg tgcacaccag cttcagccag gcggtggccg tcaccgggcg
    13501 cctggccagc accgagccca acctgcagaa catccccatc cgcacgccgg aaggccgccg
    13561 catccgcgcc gccttcatcg ccccggccgg ccaccgcatc gtctcggcgg actactccca
    13621 gatcgagctg cgcatcatgg cccacctgtc cggcgacgcc ggcctgctga acgccttcgc
    13681 ccacggcgag gacgtgcacc gggccaccgc cgccgagatc ttcggcatca cgcccatgga
    13741 agtcggcagc gagcagcgcc gcaccgccaa ggtgatcaac ttcgggctga tctacggcat
    13801 gagcgcccac ggcctggcca gaaacctggg catcgagcgg gccgccgcgg caacctacat
    13861 cgaccgctac tttgcccgtt accccggcgt cgccgcctac atggagcgca cccgcaccga
    13921 agcccggaca cagggctacg tggagaccgt cttcggccgc cgcctgtacc tgccggagat
    13981 ccgcgcccag caggtggggc ggcgccaggc tgccgaacgg gccgccatca acgcgcccat
    14041 gcagggcacc gccgcggatc tgatcaagaa ggccatgatc gccgtgtctg cctggctcaa
    14101 aacggccggc gtgcgggcgc gcctgatcct gcaggtccac gacgaactgg tgctggaagt
    14161 gcccgacgac gaggtcgctc tgatccgcga gcggctgccc cagctcatgg cgggcgtcgc
    14221 gcaactttcc gtgccgctgg ttgccgaagt gggggtcggc gcgaactggg acgaggccca
    14281 ctgatcgcaa tgcgtgcgcc gggcgcggac ggctcctccg cgcccggccc ggttcgcgcc
    14341 gaccctccgc ccaccccacg agatccgccc atgcagcacc accgctccgc ccgcctgccc
    14401 gttctgttcc tcctcagctg cgccgcgctg gcccttggcg cctgcagcag cgcccccaag
    14461 aaagacctga cctaccgcag cgagcgcttc aacaccaact cgccctacga ggtggcggtg
    14521 gacgtggcgc cgaccgcggc ctgcgacgcc ggccggcgcg ccctgctcag ccagggctac
    14581 gtgatcgagg acgccaagcc cgacgcggtc aaggcgcgca agttcttcca gcccgagcgc
    14641 aacgtgcacg tgcagatcaa cttcgtcctc tcctgcctgg cggacggcga cggcaccgaa
    14701 gtcttcgcca atgcccgcga agtccgcgac gagctcaagg ccagttccag cagcgccgga
    14761 gtcagcgtcg ccggcatcgg ctccctcaac ctgcccctgg gcggcaacgg cgaatccctc
    14821 gtcaaggtcg gtgaagagac catcaccgat tccgatttct acgggcgctt cttcgccctg
    14881 atgcagacct acatccgctg atgactcttc gctccaccct ggccgccggc gtggcggccc
    14941 tcgtcctggg cgtgcctgtc gcccgcgccg ccgatgtcgc ccagttcgcc ccctgcgtcg
    15001 cgggcctcaa ggccggcgcc gtcgcgcggg gcgtctcgcc tgccggctac gaacgcctca
    15061 ccgccggcct ggagccggat ctcaaggtgc tggattttct cgactaccag cccgaattcc
    15121 gcaccccgat ctgggattac ctctccggcc tggtggacga cgaacgcgtc gccgacgggc
    15181 aggcgatgct caagcagtgg ggcgacaccc tcgccgccgt ggctgcccgc taccacgtgg
    15241 accccgccac cgtcgtcgcc gtgtggggcg tggagagcaa cttcggccgc aacttcggca
    15301 cccgcccgct gctcacctcc ctgtctaccc tgtcctgtta cgggcgccgc caggcctact
    15361 tccgcggcga gttctacgac gcgctgaaga tcgtggacgg cggcgacgtg aaggccgagc
    15421 agctcaccgg ctcctggg
//
LOCUS       DAOQPA010000087        15193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_1984_length_15193_cov_16.165742, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000087 DAOQPA010000000
VERSION     DAOQPA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15193
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_15193_cov_16.165742"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..607)
                     /gene="ahpC"
                     /locus_tag="PKX01_12140"
     CDS             complement(44..607)
                     /gene="ahpC"
                     /locus_tag="PKX01_12140"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HMZ76924.1"
                     /translation="MSIINTEIKPFKATAYANGKFVPVSDADLKGKWSVVFFYPADFT
                     FVCPTELGDLADVYPEFQKLGVEVYSVSTDTHFVHKAWADASDTIKKIKYVMIGDPTG
                     AITRNFDVMIEEEGLALRGTFVINPEGVIKVAEIHDLGIGRDAKELLRKVQAAQYVAS
                     HPGEVCPAKWTPGDATLSPSLDLVGKI"
     gene            complement(1049..2224)
                     /locus_tag="PKX01_12145"
     CDS             complement(1049..2224)
                     /locus_tag="PKX01_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HMZ76925.1"
                     /translation="MRALLWVLGLFALAAGLAIAGRYNEGYALLVLPPYRVQISLNLV
                     LVLLLGGFVGIYALSRLVARTLALPGAVQRFRARKGRERAAQALYDAARLRIEGRFGQ
                     AMRQAAAAHEAKESPGLAALLAARAAHSLRDDERYRAWLAKAAEHDDEVRVARLMTEA
                     ELAVEGRRFEEAAERIADLQAGGQRHIAALRLALRTAQARAQWSEVVRLVRQLVKVKS
                     LKPEQAELLKRQAHQEGLRQRHGDAAALNAYWQDVPRDEQRDARVVATLARALIAAGD
                     GVAAQQAIEKQLDVEWDSVLAGLYGRCEGGEVRSRLVRAEGWLKDHPRDPQLLLTLGR
                     LCVQSQLWGKAESYLDAALAIEPGWEAHVELAQLAERVGRTDDANRHYRAAAELSRR"
     gene            complement(2227..3321)
                     /locus_tag="PKX01_12150"
     CDS             complement(2227..3321)
                     /locus_tag="PKX01_12150"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HMZ76926.1"
                     /translation="MSSELPSLPSVDAARPVDSTPPSPWRRPSAVIAAVAVALLGWQW
                     LETRSRLADLQDELARRLTDSDTAAKEGRALAKQAQEGVQDLQSKLGALEGRIAESQG
                     QQVALEAMYQEFSRTRDDRVLTEVEQAVTIAAQQLQLAGNVQAALFALQTADARLGSV
                     DRPHWLPLRKALAADMDSLKALPQIDAPGVALRLETLVGRIEGLPLAFEAHPRNEAAK
                     ADAAETSWIARLWSEFWNDFSQLVRIERLDRPDPALLAPSNVVFLRENLKLRLLNARL
                     ALMSRDGNTFREDVRVAAQWLERYFDGRNAAVATARTDLLELQKTPVGVDLPSLQASL
                     SAVQRLQAASERPVEAARPAAAAPARKSGR"
     gene            complement(3378..4151)
                     /locus_tag="PKX01_12155"
     CDS             complement(3378..4151)
                     /locus_tag="PKX01_12155"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMZ76927.1"
                     /translation="MSGLPLAGRTVAVTRPAAQADSLCAALVRLGAEPLRFPLLTITA
                     VADPAPLQAVAGHLGDFDLAFFVSPNAVNFALDALLPVAPWPARLAVATVGLGSEAAL
                     AARGFHDVIAPRAGFDSESVLALPEFQAEAVAGRRVLILRGDGGRDLLGNTLVERGAR
                     VDYLTCYHRGGPDADPAPLFEHARRGRLDALTVTSSEGVAHLLALPGWAVLADVPLFV
                     PHPRIAEAARRGGFATVIPTGPGDSGLLDGLRAHFAAAP"
     gene            complement(4173..5102)
                     /gene="hemC"
                     /locus_tag="PKX01_12160"
     CDS             complement(4173..5102)
                     /gene="hemC"
                     /locus_tag="PKX01_12160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HMZ76928.1"
                     /translation="MSAPTPDRIVIATRESRLALWQAEHVKARLQALYPTCTVELLGM
                     TTRGDQILDRPLAKVGGKGLFVKELETALLDRRADIAVHSMKDVPMELPPEFALVAIS
                     EREVPLDAFVSNKYESLDDMPPGAVVGTSSLRREAQLHAQYPMLAVTSLRGNLDTRLR
                     KLDEGQYDAIILAAAGLTRLGLASRIRAVLPSTVSLPAAGQGALGIEALADRPEVAAW
                     MAPLAEAGTAACVRAERATSRALAGSCEVPLGAYAEVQDGQLWLRGFVALPDGSRIAR
                     AERRGPLAEPEALGLALAADLRADGADEILAGL"
     gene            5318..8065
                     /gene="ppc"
                     /locus_tag="PKX01_12165"
     CDS             5318..8065
                     /gene="ppc"
                     /locus_tag="PKX01_12165"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HMZ76929.1"
                     /translation="MSEDKDLPLRDDIRLLGRVLGDTLRAQEGEAVFDLVETIRQSSI
                     RFRRHADPAARKELEATLDSLSRDQTIDVVRAFSYFSHLSNIAEDQHHIRRSRAHQLA
                     GSAPREGSLAHALERAFAAGQGTPELAAFFDTARVTPVLTAHPTEVQRKSILNCQMVI
                     ARLLDERDRMQLTPEEEQANDEALRRAVLTLWQTRMLRTEKLSVMDEVSNGLSYFDYT
                     FLRELPRLYANLEDRLATRDRSWGALELPNFLQVGSWIGGDRDGNPFVTAEILEKALA
                     VQAEKVLTFYMDEVHTLGSQLSVAQGLVSASDALLRLAEASPDGSPHRIDEPYRRALT
                     GLYARLAATYAHLLGHTPPRHAVGPAAPYSHVDAFADDLEIVHRSLLANGLGALARGR
                     LRHLRRAVGVFGFHLAPIDLRQNSDVHERVVAELLRAITPDTDYLALDEAGRCALLLD
                     ELATPRPLASPWIDYSEESRGELAIFRAARAAHLRYGRAAVPNCIISKTDDVSDLLEV
                     AVLAKEAGLLRPAEGVLDVNIIPLFETIGDLRNAAGVMDRLFQLPGYRALLDSRHGEQ
                     EVMLGYSDSNKDGGFLTSGWELYKAEIGLVEVFARHGVRLRLFHGRGGSVGRGGGPSY
                     QAILAQPGGAVQGQIRLTEQGEVIASKYANPEVGRRNLEVLAAATLEATLFAPRDPAP
                     RPDYLEAMDELSADAFAAYRTLVYETDGFERYFWESTVIAEIAALNIGSRPASRKKST
                     AIEDLRAIPWVFSWAQCRLMLPGWYGFGSAVRAYLERHGDAGRARLQAMHKEWSFFAT
                     LLSNMDMVLAKSDIAIASRYAELVRDTALRDAIFPRIRQEHADTLEVLLAITGQQELL
                     DANPLLKRSMRNRFPYLDPLNHVQVELLHRYREGHQDERVRRGILLSINGIAAGLRNS
                     G"
     gene            8225..9280
                     /locus_tag="PKX01_12170"
     CDS             8225..9280
                     /locus_tag="PKX01_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HMZ76930.1"
                     /translation="MALHFAVAIGVLIVYACLHPFTGWRDPGLPLLDFLTAPWPRYYS
                     QTDLIVNVLGFMPFSFALVPALGGRLRPGGAVCMAVVIAGLLSLGLETLQNFLPSRVP
                     SNVDLGCNALGGLIGALAGARWGAPLFDTRGALARWRTRRIVAGHVGEAGMVLMGLWL
                     LTLLTPESLLFATGDLRRLFDLPTPIPFNPQRFVKVEAAIAASQLVAVGLTARCMMRS
                     PSPFPILLLVLLGLGAKSLAAGMFFSPGDPFHWATPGGQIGIAAGAALLTACLILPTR
                     LHAVLAGLALLVGTALVNLAPENPFLPNDTLLALRGNLLNFHGLTSLAASLWPFLAFL
                     YLGLLGGRSGRDGAGPH"
     gene            9346..9654
                     /locus_tag="PKX01_12175"
     CDS             9346..9654
                     /locus_tag="PKX01_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HMZ76931.1"
                     /translation="MSYFKHHVFICCNQRPEGETCCNARGGSELQAYAKDRIAALGLK
                     GRGKVRINKAGCLDRCDEGPVLVVYPDEVWYTFVDKADIDEIIHEHLQHGRIVERLKI
                     "
     gene            9661..10308
                     /locus_tag="PKX01_12180"
     CDS             9661..10308
                     /locus_tag="PKX01_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ76932.1"
                     /translation="MNRPAATESALINGPAGAIELLIDTPADVRGIALVCHPHPLFHG
                     ANTNKVAHTLARVLRDLGYAVVRPNFRGVGKSAGTHDNGHAETDDMLAVLSWAESRWG
                     SLPIALGGFSFGAYVQTRVAARLAESVAPAQRIVLVGTAAGYVTGARSYTTLPVPADT
                     LVIHGEKDETVPLANVMEWAEPQEIPVVVIPGADHFFHGRLHILRTVIERAWTPL"
     gene            10337..10945
                     /locus_tag="PKX01_12185"
     CDS             10337..10945
                     /locus_tag="PKX01_12185"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HMZ76933.1"
                     /translation="MKLIVSLTSPFARKIRVILAEKGLPCDLQVDIPWLAETNVPDYN
                     PLGKVPVLVADDGEVWFDSPVIAEYLETLGGPALLPADRIASLPVRQSEALADGITDA
                     AVSAFLEMRRPAPQQDPASLERQITKIRRGLGALEERFAARKGFGGDTLTLADIAVGC
                     ALGYLDLRFAHLDWRAGRPALAAWAAGLLARPSFVASVPPAV"
     gene            10945..11883
                     /locus_tag="PKX01_12190"
     CDS             10945..11883
                     /locus_tag="PKX01_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ76934.1"
                     /translation="MPLEIAGLVKRFGHGADAREVVRGLDLSLRPGECFALLGPNGAG
                     KTTTLRCALGLTAASAGSIRLCGLPVPAQAREARARVGVVPQLDSLDPDFTCAENLLV
                     FAGYFGIPDSEIRPRIPELLAFAGLEGKENARIPSLSGGMKRRLTLARALVNRPELLI
                     LDEPTTGLDPQARHLIWERLRRLVAEGTTVLLTTHFMDEAERLADRLAILDGGRILAQ
                     GSPREVIAAHIEPQVVEVFGDWADAPGGAPAWASRHAAALARRCEVSGETAFCYTDDA
                     APLLAHLAHQSGLRTLHRPANLEDVFLKLTGRELRD"
     gene            11980..13320
                     /locus_tag="PKX01_12195"
     CDS             11980..13320
                     /locus_tag="PKX01_12195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ76935.1"
                     /translation="MSACPDPRTIIEHLPVALAVARATDGRLLSSNRGFAALFGAPAT
                     LGMPLTDWLRHADAGDRIGPPPDWPAHLAALHPDIRPHDSVECLLRGPAGPLHIRVQL
                     QVADGLLIACFVDLTDHETREDILRQALAELETRSATDALTGLLTRRRFEEAAASEMQ
                     RFRRFGHPITLIMADIDHFKLINDRLGHQAGDRVLVEFARRILAECRDLDVIGRYGGE
                     EFLVLLPSTPLVGGLTLAAKLRQAIAAAPFPDAGPLSASFGVAECQADEDWHAWLHRT
                     DKALYRAKRQGRNLVVAAPERHDQPHAHAELSRLDRLGWEDGHTLGHPLMDHQHKELF
                     TLAQALLEMIRRDGPTADLHVAIRELAHTTERHFRDEEALLGEIAYPRTDQHMKAHRL
                     LAAKLDALSSQSARGVLPAARLIEFLAYDLVDHHALGPDRDYLPWLDGRTPETP"
     gene            13317..14102
                     /locus_tag="PKX01_12200"
     CDS             13317..14102
                     /locus_tag="PKX01_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ76936.1"
                     /translation="MNPPQPAPPRLSGRIAAVWRRNFLVWRKLALPSIVGNLADPVIY
                     MLGLGYGLGMLVPEVGGQSYIAFLAGGTLCYSTMNAATFEVLYSSFSRMHVQKTWDAI
                     LNAPVSLDDIVAAELLWAASKATMAGLAILAVIALFGYTASPLALAALPVVFLTGLAF
                     AALGLIMTALAPGYDFFTYYFTLFITPMTLLSGVFFPRDQLPGGFQLLAELLPLSHAV
                     ALARPLLQGHWPVHAALHLVVLSACAGIAFAVARVLMRRRLLK"
     gene            14112..14444
                     /gene="cutA"
                     /locus_tag="PKX01_12205"
     CDS             14112..14444
                     /gene="cutA"
                     /locus_tag="PKX01_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HMZ76937.1"
                     /translation="MADPDILLVFTTLPDADSAARIARQLVDARLAACVSIQAPCTSV
                     YRWQGQTETATEVPLLIKTPRARYAALQETLRAAHPYELPEIVAVPVSEGLPAYLAWV
                     AGETAPAS"
     gene            14491..>15193
                     /locus_tag="PKX01_12210"
     CDS             14491..>15193
                     /locus_tag="PKX01_12210"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HMZ76938.1"
                     /translation="MNRAPLRLFALLLPALLMLGLLVARPAAAAEPLPAEQAFHLDAR
                     ALDDRTVEIRFQVAEGYYLYKHKFKFEADGVTFGEAQLPPGKRKKDDIYGEVETYRGE
                     LVFPLPLSAGQPPFKLTVTSQGCADLGICYPPTPQTVSIDPAGQRKVGGFLARALGQA
                     PAAEAPLTAPASTTAVPADAHDESGRIARLLAGGGTLATLASFFGFGLLLAFTPCVFP
                     MIPILSGIIVGHGHAI"
BASE COUNT         2152 a         5762 c         5063 g         2216 t
ORIGIN      
        1 gctgcccggg cgttgcggta cgacaggtgt ccgggcaggc ggcttagatc ttgccgacca
       61 ggtccaggga cggggacagg gtggcgtcgc ccggggtcca cttggcgggg cagacttcgc
      121 ccgggtggga ggccacgtac tgggcggcct gcaccttgcg cagcagttcc ttggcgtcac
      181 ggccgatgcc caggtcgtgg atttcggcga ccttgatgac gccttcgggg ttgatcacga
      241 aggtgccgcg cagggccagg ccttcttctt cgatcatcac gtcgaagttg cgggtgatgg
      301 cgccggtggg gtcgccgatc atcacgtact tgatcttctt gatggtgtcg gaggcgtcgg
      361 cccaggcctt gtgcacgaag tgggtgtcgg tggagacgga gtacacctcg acgcccagct
      421 tctggaattc ggggtagacg tcagccaggt cgcccagctc ggtggggcag acgaaggtga
      481 agtcggcggg gtagaagaac accacggacc acttgccctt caggtcggca tcggaaaccg
      541 ggacgaactt gccgttggcg taggcggtgg ccttgaaggg tttgatttcg gtgttgatga
      601 tggacatggg ctttccttca tgtggagtgg gatgcggaga cgccatgata agagggctgc
      661 gccgcgtact caaattgact ctgaaaatga tcccgattaa agaaagctat cggtttcgcc
      721 attatggctg aaatatgacc gtgctggcgg gacgcgctcg cccaggacaa ggggcctgtg
      781 cggcggccga cgtgctcgat gcgggccgga acgcggccca tggtgcgccc ccggcaggaa
      841 cgcagacggt gagccgggcg tcagccgcgg acagtgaggc cacacggcgc aggggccggg
      901 cggcggggcg gggtctatcc tcgacggggt cggacgaagg cacacctggc gcgccggctg
      961 tggcgccggg cgatgcctgc cagacagggg ccccgcaggc atcgggctga gatgcgggat
     1021 cggagtggat cgttccgccg cggggcgctc agcgccggga cagctcggcc gcggcgcggt
     1081 agtggcggtt ggcgtcgtcg gtgcggccga cgcgctcggc cagctgggcc agctccacgt
     1141 gggcttccca gcccggctcg atggccagcg cggcgtccag gtagctttcg gccttgcccc
     1201 acagctggga ctggacgcac aggcggccca gggtgaggag cagctgcggg tcgcggggat
     1261 gatccttgag ccagccctcg gcgcgcacca gccggctgcg cacctcgccg ccctcgcagc
     1321 ggccgtagag gcctgcgagc acggaatccc attccacgtc gagctgcttc tcgatggcct
     1381 gctgggcggc cacgccatcg ccggcagcga tcagggcgcg ggccagggtg gccaccacgc
     1441 gggcgtcgcg ctgctcgtcc cgcggcacgt cctgccagta ggcgttgagc gcggcggcgt
     1501 cgccgtggcg ctggcgcagc ccttcctggt gggcctggcg cttgaggagc tcggcctgct
     1561 cgggtttgag ggacttgacc ttgacgagct ggcgcaccag ccgcaccacc tcgctccact
     1621 gggcgcgggc ctgggcggtg cgcagggcga gacgcagggc ggcgatgtga cgctggccgc
     1681 cggcctggag gtcggcgatg cgctcggcgg cctcctcgaa gcggcggcct tcgacagcca
     1741 gctcggcttc ggtcatgagg cgggcgacgc gcacttcgtc gtcgtgctcg gcggccttgg
     1801 cgagccacgc ccggtagcgc tcgtcgtccc gcagggaatg ggcggcgcgg gcagcgagga
     1861 gtgcggcgag tccgggggat tccttcgctt catgggcggc cgccgcctgg cgcatggcct
     1921 ggccgaagcg cccttcgatg cgcagacggg cggcatcgta gagggcctgg gcggcgcgct
     1981 cacgcccctt gcgggcacgg aagcgctgca ccgcgccggg cagggcgagg gtccgggcca
     2041 ccagccgcga gagggcgtaa atccccacga aaccgcccag cagcagcacg agcacgaggt
     2101 tgagggagat ctgcacccgg tagggcggca gcacgagcag cgcgtagcct tcgttgtaac
     2161 gaccggcgat ggccagcccg gcggccagcg caaagaggcc cagcacccac agcaaggccc
     2221 gcatgatcag cgccccgact tgcgggccgg cgccgcggca gccggcctgg ccgcctcgac
     2281 ggggcgttcg gaggcggcct gcaggcgctg caccgcgctg aggctggcct gcaggctggg
     2341 caggtccacc cccaccggcg tcttctgcag ctcgagcaga tccgtgcggg cggtggcgac
     2401 ggcggcgttg cgcccgtcga aatagcgctc cagccactgg gcggccaccc gcacgtcttc
     2461 gcggaaggtg ttgccgtccc gcgacatcag ggcgaggcgg gcattgagca ggcgcagctt
     2521 gaggttctcc cgcaggaaga ccacgttgct cggcgcgagg agggccggat cggggcggtc
     2581 gagacgctcg atgcgcacga gctggctgaa gtcgttccag aactccgacc acaggcgcgc
     2641 gatccaactg gtctcggccg cgtcggcctt ggcggcttcg ttgcggggat gggcctcaaa
     2701 ggcgaggggc aggccttcga tgcggccgac cagggtctcc aggcgcaggg cgacgcccgg
     2761 tgcgtcgatc tggggcaggg ccttgaggct gtccatgtcg gccgccaggg ccttgcgcag
     2821 gggcagccag tggggacgat cgacgctgcc caggcgcgcg tcggcggtct gcagggcaaa
     2881 cagggcagcc tgcacgttgc cggccagctg aagctgctgg gcggcgatgg tgacggcctg
     2941 ctccacttcg gtcaacaccc ggtcgtcgcg ggtgcgggag aattcctggt acatggcctc
     3001 cagcgccacc tgctggccct gggattcggc gatgcgacct tcgagggcgc caagcttgga
     3061 ctgaagatcc tgcacgcctt cctgggcctg cttggccagc gcgcggcctt ccttggcggc
     3121 ggtgtcgctg tcggtgaggc gccgcgccag ttcgtcctgc aggtcggcga gacggctgcg
     3181 ggtctcgagc cattgccagc ccagcagggc gacggccacg gcggcgatca cggcggacgg
     3241 gcgccgccac ggactgggcg gcgtggaatc gacgggacgg gcggcgtcca cggacgggag
     3301 gctggggagt tcgctgctca tggcgtgagt ttaacccgga taggacgtcg acgaaggggg
     3361 agcagtcgcg acacgcttca gggcgcggcg gcaaagtggg cgcgcaggcc gtcgaggaga
     3421 ccgctgtcgc cggggccggt ggggatcacc gtggcaaatc caccgcgccg ggcggcctcg
     3481 gcgatgcgcg gatggggcac gaagaggggc acgtctgcca gcacggccca gcccggcagg
     3541 gcgagcagat gggcgacgcc ttcgctgctg gtcaccgtga gggcgtcgag gcggcccctg
     3601 cgggcgtgct cgaagagggg cgccgggtcg gcgtccgggc cgccccggtg gtagcaggtg
     3661 aggtagtcca cgcgggcgcc acgctcgacg agggtgttgc cgagcaggtc gcgcccgccg
     3721 tcgccgcgca ggatcagcac gcgccggccg gccacagcct cggcctggaa ttccggcagg
     3781 gccagcaccg actcgctgtc gaagccggcg cgcggcgcga tcacgtcatg gaagccgcgc
     3841 gccgccagag ccgcctcgct gcccagtccg accgtggcga cggcgagccg ggccggccag
     3901 ggggccaccg gcagcagcgc gtcgagggcg aaattgaccg cgttgggact gacgaagaag
     3961 gccagatcga agtcgcccag atgcccggcg acggcctgca gcggggccgg atcggccacc
     4021 gccgtgatgg tgagcagcgg aaaacgcagg ggctctgcgc ccagccggac cagcgccgca
     4081 cacaggctgt cggcctgggc ggccgggcgg gtgacggcga cggtgcgtcc ggcgaggggc
     4141 aggccgctca tgggacgctc cgcgggcggc gctcagaggc cggcgaggat ctcgtcggcg
     4201 ccgtcggcgc gcaggtcggc ggccagggcc aggccgaggg cttccggttc ggcgagggga
     4261 ccacgacgct cggcgcgggc gatgcgggag ccatcgggca gggcgacgaa gccgcgcagc
     4321 cagagctggc cgtcctgcac ctcggcgtag gcgcccaggg gcacctcgca gctgcccgcc
     4381 agggcacggg aggtggcccg ctcggcgcgc acgcaggcgg cggtgccggc ttcggcgagg
     4441 ggcgccatcc aggcggcgac ttcggggcgg tcggccaggg cctcgatgcc cagggcgccc
     4501 tggccggcgg ccggcaggga gaccgtggag ggcagcaccg cgcggatgcg cgaggccagg
     4561 ccgaggcgcg tgaggccggc ggcggcgagg atgatggcgt cgtactggcc ttcgtccagc
     4621 ttgcgcaggc gggtgtccag attgccgcgc aggctggtga cggcgagcat cggatactgg
     4681 gcgtggagct gggcctcgcg gcgcaggctg gaggtgccca ccacggcgcc cggcggcatg
     4741 tcgtcgaggc tttcgtactt gttggagacg aaggcgtcca gcggcacctc ccgttcggag
     4801 atggccacca gggcgaactc cggcggcagc tccatgggca cgtccttcat ggaatgcacg
     4861 gcgatgtcgg cgcggcggtc cagcagggcg gtttccagtt ctttgacgaa caggcccttg
     4921 ccgccgacct tggcgagggg ccggtcgagg atctggtcgc cgcgggtagt catgcccagc
     4981 agctcgaccg tgcaggtcgg gtagagggct tggaggcggg ccttgacgtg ctccgcctgc
     5041 cacagggcga ggcggctttc acgggtggcg atgacgatgc ggtcgggcgt aggggcgctc
     5101 acggtagtcg gctcggcggt cgtggggagc ccgggcgggg ctccggttgg cgttcggggg
     5161 atggacaaga ccgggcggag ggcgcagatt gcgcgctgat gcagcgcagc atcagggttg
     5221 ccaccaatgg cgtccatcgg gtcgcggatt ctagcatgga gcccttgacc ccgcggcccg
     5281 ctgccggcgg gccccctcga accttccagg actccccatg agcgaagaca aggacctccc
     5341 cctccgtgac gatatccgtc tgcttggccg cgtgctgggc gacaccctgc gcgcccagga
     5401 gggcgaagcg gtgttcgacc tggtggagac gatccgccag agctccatcc gcttccgccg
     5461 ccacgccgac ccggcggccc gcaaggagct ggaagccacg ctggattccc tgtcccgcga
     5521 ccagaccatc gacgtggtgc gtgcgttcag ctacttctcg cacctctcca acatcgccga
     5581 ggaccagcac cacatccgcc gctcccgcgc ccaccagctg gccggctcgg cgccgcggga
     5641 aggcagcctc gcccacgccc tggagcgcgc cttcgccgcc ggccagggca cgcccgagct
     5701 ggcggccttc ttcgacacgg cgcgggtcac cccggtgctc accgcccacc ccaccgaagt
     5761 gcagcgcaag agcatcctca actgccagat ggtgatcgcg cgcctgctcg atgaacggga
     5821 ccgcatgcag ctcaccccgg aagaggagca ggccaacgac gaggccctgc gccgcgccgt
     5881 gctgaccctg tggcagacgc gcatgctgcg taccgagaag ctgtcggtga tggacgaggt
     5941 gagcaacggc ctgtcctact tcgactacac cttcctgcgg gagctgccgc gcctctacgc
     6001 caacctggaa gaccgcctcg ccacccgcga tcgcagctgg ggcgcactgg agctgcccaa
     6061 cttcctgcag gtgggcagct ggatcggcgg cgaccgggac ggcaacccct tcgtcaccgc
     6121 cgagatcctc gaaaaggccc tcgccgtgca ggccgagaag gtcctcacct tctacatgga
     6181 cgaggtgcac accctgggct cccagctctc cgtcgcccaa gggctggtga gcgcctcgga
     6241 cgccctgctg cgcctcgccg aagcctcgcc ggacggctcg ccccaccgca tcgacgagcc
     6301 ctaccggcgc gccctcaccg gcctctacgc gcgcctcgcc gccacctacg cgcacctgct
     6361 cggccatacc ccgccgcgcc atgcggtggg ccccgcagcg ccctattccc atgtggacgc
     6421 ctttgccgac gacctggaga tcgtgcatcg ctccctcctg gccaacggcc tgggcgccct
     6481 ggcccgcggc cgcctgcgcc acctgcgccg ggcggtgggg gtgttcggct tccacctggc
     6541 gcccatcgac ctgcgccaga actccgacgt gcacgagcgt gtggtggctg agctgctgcg
     6601 cgccatcacc ccggacaccg actacctggc cctcgacgag gccggccgct gcgccctgct
     6661 gctggacgaa ctggccaccc cgcggcccct cgcctcgccg tggatcgact attccgagga
     6721 atcccgcggc gagctggcga tcttccgcgc cgcccgcgcc gcccacctgc gctatggccg
     6781 ggcggcggtg cctaactgca tcatctccaa gaccgacgac gtctccgacc tgctggaggt
     6841 ggccgtgctc gccaaggagg ccggcctgct gcgcccggcc gagggcgtgc tggacgtgaa
     6901 catcatcccg ctcttcgaga ccatcggcga cctgcgcaac gccgccggcg tcatggaccg
     6961 cctgttccag ctgcctggct accgggccct gctggattcc cgccacggcg agcaggaggt
     7021 gatgctgggc tactccgaca gcaacaagga cggcggcttc ctcacctccg gctgggagct
     7081 ctacaaggcc gagatcggcc tggtggaggt cttcgcccgc cacggcgtgc gcctgcgcct
     7141 gttccacggc cgcggcggct ccgtcgggcg cggcggcggc cccagctacc aggccatcct
     7201 ggcccagccg ggcggcgccg tgcagggcca gatccgcctg accgagcagg gcgaggtcat
     7261 cgcctccaag tacgccaacc cggaagtggg ccggcgcaac ctggaagtgc tcgccgccgc
     7321 caccctggag gccaccctct tcgccccccg cgacccggcc ccgcgccccg actatctgga
     7381 ggccatggac gagctgtccg ccgacgcctt cgccgcctac cgcacgctgg tctacgagac
     7441 cgacggcttc gagcgctact tctgggagtc cacggtgatc gccgagatcg ccgccctcaa
     7501 catcggcagc cgcccggcct cgcgcaagaa gtccaccgcc atcgaggacc tgcgcgccat
     7561 cccgtgggtc ttcagctggg cccagtgccg cctcatgctg cccggctggt acggcttcgg
     7621 ctcggccgtg cgggcctacc tggaacgcca cggcgacgcc ggccgggcgc gcctgcaggc
     7681 catgcacaaa gaatggtcct tcttcgccac cctgctctcc aacatggaca tggtgctggc
     7741 caagagcgac atcgccatcg ccagccgcta cgccgagctg gtccgcgaca ccgccctgcg
     7801 cgatgcgatc ttcccgcgca tccgccagga acatgccgac acgctggagg tgctcctggc
     7861 catcaccggc cagcaggaac tgctcgacgc caacccgctc ctcaagcgct ccatgcgcaa
     7921 ccgcttcccc tacctggatc ccctcaatca cgtccaggtg gagctgcttc accgctatcg
     7981 ggaaggccac caggacgagc gcgtgcggcg cggcatcctg ctctccatca acggcatcgc
     8041 cgccggcctg cgcaacagcg gctgagggtt gcggcccccc ggagcggcgg gcccggccgc
     8101 tccggggcga tttgctgcgc cgcacacgcc aaacggacag ggcggcccgt cccggcctaa
     8161 aatccggatg tcgcccgccc gccgcgccga gatgcccatc cccttgcgtt cacgtccgtc
     8221 ccccatggcc ctgcatttcg cggtggccat cggcgtgctc atcgtgtacg cctgcctgca
     8281 ccccttcacg ggctggcgcg atccgggcct gccgctcctc gacttcctga ccgcaccgtg
     8341 gccacgctat tacagccaga cagacctgat cgtgaatgtg ctgggcttca tgcccttcag
     8401 cttcgccctt gtcccggccc tgggcgggcg cctgcggccc ggcggcgcgg tgtgcatggc
     8461 ggtggtgatt gccggactcc tcagtctcgg cctggagacg ctgcagaact tcctgcccag
     8521 tcgcgtcccc tccaatgtgg atctgggctg caacgccctc ggggggctca tcggagccct
     8581 cgccggcgcc cgctgggggg cgcccctgtt tgacacccgc ggcgccctgg cgcgctggcg
     8641 cacgcgccgc atcgtggccg ggcacgtggg cgaggccggc atggtgctga tgggcctgtg
     8701 gctgctcacc ctgctcacgc cggagagcct gctcttcgcc accggcgacc tgcgccgcct
     8761 gttcgacctg cccacgccca tccccttcaa cccccagcgc ttcgtcaagg tggaggccgc
     8821 catcgccgcc agccagctgg tcgccgtggg cctcaccgcg cgctgcatga tgcgcagccc
     8881 gagccccttc ccgatcctac tgctggtgct cctcgggctg ggggcaaaaa gcctcgccgc
     8941 gggcatgttc ttttcgcccg gcgacccctt ccactgggcg acgcccggcg gacagatcgg
     9001 catcgccgca ggcgccgccc tgctcaccgc ctgcctgatc ctgcccaccc gcctgcacgc
     9061 ggtcctggcc gggctggccc tgttggtggg caccgccctg gtcaatctcg ccccggaaaa
     9121 ccccttcctg ccgaacgaca ccctgctcgc cctgcggggc aacctcctca acttccatgg
     9181 gctgacctcc ctcgccgcga gcctgtggcc cttcctcgcc ttcctctatc tgggcctgct
     9241 gggcggccgc agcggacggg acggggccgg cccccactga gccgcgcccg ccgggcggcg
     9301 tttggacgat aatcgcaggc gccccttacc ggagaatccc gccccatgag ctacttcaag
     9361 caccacgtgt tcatctgctg caaccagcgc cccgaaggcg agacctgctg caacgcccgc
     9421 ggcggctccg aactgcaggc ctacgccaag gaccgcatcg ccgccctcgg tctcaagggc
     9481 cgtggcaagg tgcgcatcaa caaggccggc tgcctcgacc gctgtgacga agggccggtg
     9541 ctggtggtct acccggacga ggtctggtac accttcgtgg acaaggccga catcgacgag
     9601 atcatccacg aacacctgca gcacggccgc atcgtcgaac gtctcaagat ctgagccgcc
     9661 atgaaccgcc ccgccgccac cgaatccgcc ctcatcaacg gtcccgccgg ggccatcgag
     9721 ctgctcatcg acacccccgc cgacgtgcgc gggattgcgc tggtctgtca cccccatccg
     9781 ctcttccacg gcgccaacac caacaaggtg gcccacaccc tggcgcgggt gctgcgcgac
     9841 ctgggctacg ccgtcgtccg ccccaacttc cgcggcgtgg gcaagagcgc cggcacccac
     9901 gacaacggcc atgccgagac cgacgacatg ctggccgtgc tgagctgggc cgagagccgc
     9961 tggggcagcc tgcccatcgc cctgggcggc ttctccttcg gcgcctacgt gcagacccgc
    10021 gtcgccgcgc ggctcgccga gagcgtcgcg ccggcccagc gcatcgtgct ggtgggcact
    10081 gcggccggct atgtcacggg cgcccgcagc tacaccacac tgccggtccc ggccgacacc
    10141 ctggtcatcc atggcgagaa ggacgagacc gtgcccctcg ccaacgtgat ggaatgggcg
    10201 gaaccccagg aaatcccggt ggtcgtcatt ccaggcgccg accatttctt ccatggccgc
    10261 ctgcacatcc tcagaaccgt catcgaacgg gcctggacgc ccctctgagc cccgaccccc
    10321 gacaggaacc ccgcccatga agctcatcgt ttcgctcacc agcccgttcg cacgcaagat
    10381 ccgcgtgatc cttgccgaaa aaggcctgcc ctgcgacctg caggtggaca tcccgtggct
    10441 ggccgagacc aacgtgcccg actacaaccc cctcggcaag gtgcccgtgc tggtggcgga
    10501 cgacggcgag gtgtggttcg actcgccggt catcgccgaa tacctggaaa ccctcggcgg
    10561 ccccgccctg ctgcccgccg accgcatcgc ctcgctgccg gtacgccaga gcgaggccct
    10621 cgccgacggc atcaccgacg cggcggtgag cgccttcctg gagatgcgtc gccccgcccc
    10681 ccagcaggac ccggccagcc tggagcgcca gatcaccaag atccgccgcg gcctgggcgc
    10741 actggaagaa cgcttcgccg cccgcaaggg cttcggcggc gacaccctga ccctcgccga
    10801 catcgccgtg ggctgcgccc tgggttatct ggacctgcgc ttcgcccacc tggactggcg
    10861 cgccggccgt cccgccctgg ccgcctgggc cgccggcctg ctggcccggc cgtccttcgt
    10921 cgccagcgtg ccgccggccg tctgatgccc ctcgagatcg ccggtctcgt caagcgcttc
    10981 ggccatggcg ccgacgccag ggaggtggtg cgcgggctcg acctgagcct gcgcccgggc
    11041 gaatgcttcg ccctgctcgg ccccaacggc gccggcaaga ccaccaccct gcgctgcgcc
    11101 ctcggcctca ccgcggccag cgccggcagc atccgcctgt gcggcctgcc ggtgccggcc
    11161 caggcgcgcg aagcccgcgc ccgcgtcggc gtggtccccc agctcgacag cctcgacccg
    11221 gatttcacct gcgccgagaa cctcctcgtc tttgccggct acttcggcat cccggacagc
    11281 gagatccgcc cgcgcatccc cgagctcctc gccttcgccg gcctggaagg caaggaaaac
    11341 gcccgcatcc cgtccctctc cggcggcatg aagcggcgcc tgaccctggc ccgcgccctg
    11401 gtcaaccgtc ccgaactgct gatcctcgac gagcccacca ccggcctcga cccgcaagcc
    11461 cgccacctca tctgggaacg cctgcgccgc ctcgtcgccg aaggcaccac ggtgctcctc
    11521 accacccact tcatggacga ggccgagcgc ctcgccgacc gcctcgccat cctcgacggc
    11581 ggccgcatcc tggcccaggg cagcccgcgg gaggtcatcg ccgcccacat cgaaccccag
    11641 gtggtggaag tcttcggcga ctgggccgat gcccccggcg gcgccccggc gtgggcgtcc
    11701 cgccatgccg cggccctcgc ccggcgctgc gaggtgagcg gcgagacggc cttctgttac
    11761 accgacgacg cggccccgct gctcgcccat ctggcccacc agagcggcct gcgtaccctg
    11821 caccggcccg ccaatctcga ggacgtcttc ctcaagctga ccggccgcga gctgcgcgac
    11881 tgaggtgcgc cgcatacccc ggctatgagc ggggttttcc ccacaggacg ggggataggc
    11941 ggtacagtcc cgcctatcca cgccccaaca agaacaacca tgtccgcgtg ccccgacccc
    12001 cgaacgatca tcgagcacct gccggtcgcc ctggcggtcg cccgtgccac tgacggtcgg
    12061 cttctgagct cgaaccgggg cttcgcggcc ctcttcggcg cccccgccac cctcgggatg
    12121 ccgctcaccg actggctccg ccacgccgat gccggcgacc gcatcggccc gccgccggac
    12181 tggccggcgc acctggccgc gctgcacccg gacatccggc cccacgacag cgtcgaatgc
    12241 ctgctccgcg gccccgccgg cccgctccac atccgtgtcc agctgcaggt ggcggacggc
    12301 ctgctcatcg cctgcttcgt ggacctcacc gatcacgaaa cccgcgaaga catcctgcgc
    12361 caggccctgg ccgaactcga aacccgctcc gcgaccgacg ccctgaccgg cctgctcacc
    12421 cggcgccgct tcgaggaagc cgccgccagc gagatgcagc gcttccgccg cttcggtcat
    12481 ccgatcaccc tgatcatggc ggacatcgac cacttcaagc tcatcaacga ccgcctcggt
    12541 caccaggccg gcgatcgggt gctggtggaa ttcgcccgcc gcatcctggc cgaatgccgc
    12601 gacctggacg tgatcggccg ctacggcggt gaagagttcc tcgtcctgtt gcccagcacc
    12661 cccctcgtcg gcggcctgac cctggcggcc aagctgcgcc aggccatcgc cgcggccccc
    12721 ttcccggacg ccggtccgct cagcgccagc tttggcgtgg ccgaatgtca ggcagacgag
    12781 gactggcacg cctggctgca ccgcaccgac aaggccctct accgggccaa gcgccagggc
    12841 cgcaacctgg tcgtcgcggc gcccgagcgc catgatcagc cccacgccca cgccgagctg
    12901 tctcgtctcg accggctcgg ctgggaagac gggcacacgc ttggccatcc cctcatggac
    12961 catcagcaca aggaactctt caccctcgcc caggccctgc tggagatgat ccggcgcgac
    13021 ggccccaccg ccgacctcca cgtggcgatc cgcgaactgg cccacaccac ggagcgccac
    13081 ttccgtgacg aggaggccct cctcggcgag atcgcctacc cccgcacgga ccagcacatg
    13141 aaggcgcacc gcctcctcgc cgccaagctc gacgccctct cgtcccagag cgcccgcggc
    13201 gtgctgcccg ccgcacgcct catcgagttc ctcgcctacg acctggtgga ccaccacgcc
    13261 ctcggacccg accgggacta cctcccgtgg ctcgacggcc gcacgccgga gaccccatga
    13321 acccccccca gcctgcccca ccgcgcctgt ccggccgcat cgccgccgtg tggcgccgca
    13381 acttcctggt ctggcgcaag ctggcgcttc cctccatcgt cggcaacctg gccgatccgg
    13441 tgatctacat gctgggtctc ggctacggcc tgggcatgct ggtgcccgag gtcggcggcc
    13501 agtcctacat cgccttcctg gccggcggca ccctgtgcta ttccacgatg aacgcggcca
    13561 ccttcgaggt gctgtactcc agcttctcgc gcatgcacgt acagaaaacc tgggacgcca
    13621 tccttaacgc ccccgtgtcc ctcgatgaca tcgtcgccgc cgaactcctg tgggccgcca
    13681 gcaaagccac catggccggc ctcgccatcc tcgcggtgat cgccctgttc ggctacaccg
    13741 cctcgcccct ggccctcgcc gccctgccgg tggtcttcct caccggcctc gccttcgccg
    13801 ccctcggcct gatcatgacc gccctggcgc caggctacga cttcttcacc tactacttca
    13861 ccctcttcat cacgcccatg accctcctgt ccggcgtgtt ctttccccgc gaccagctgc
    13921 ccggcggctt ccagctcctc gccgagctgc tccccctcag ccacgccgtg gcccttgccc
    13981 gcccgctgct gcaggggcac tggcccgtcc acgccgcgct gcatctggtc gtcctgagcg
    14041 cctgcgcggg catcgctttc gccgttgccc gggtcctgat gcggcggcgt ctgctgaagt
    14101 aaactccgcc catggccgac cccgacatcc tgctcgtttt caccaccctg cccgacgccg
    14161 acagcgctgc acgcatcgcc cgccagctgg tggacgcccg cctcgccgcc tgcgtgagca
    14221 tccaggcgcc gtgcacctcc gtctatcgct ggcaggggca gaccgagacc gccaccgagg
    14281 ttccgctcct catcaagacc ccccgcgcac gctacgccgc cctgcaggag accctgcgcg
    14341 cagcccatcc ctacgaactt ccggagatcg tcgcagtccc tgtcagcgag ggcctgcccg
    14401 cctacctcgc ctgggtggcc ggcgaaaccg ctccggcttc ttgatctccg tcggcgcgcc
    14461 cgtcgccggc acccgctaca cttgcccgcc atgaaccgcg ctccgctccg cctcttcgcc
    14521 ctgctgctgc ccgccctgct gatgctgggc ctccttgtcg cccgtccggc cgccgcggcc
    14581 gaaccgctgc ccgccgagca ggccttccac ctggacgccc gcgccctcga cgaccgcacc
    14641 gtcgagatcc gcttccaggt cgccgagggc tattacctct acaagcacaa gttcaagttc
    14701 gaggccgacg gcgtcacctt cggcgaggcc cagcttccgc ccggcaaacg caagaaggac
    14761 gacatctacg gcgaagtcga aacctaccgg ggcgaactgg tcttccccct gcccctcagc
    14821 gccggccagc cgcccttcaa gctcaccgtc accagccagg gctgcgccga cctgggcatc
    14881 tgctacccac ccacccccca gacggtgagc atcgaccccg cgggacagcg caaggtcggt
    14941 ggcttcctcg cccgcgccct cggccaggca ccggccgccg aggccccgct cactgcaccg
    15001 gcatccacca ccgccgtgcc ggccgacgcc cacgacgaat ccggccgcat cgcccgcctg
    15061 ctggccggtg gcggcaccct cgccaccctc gccagcttct tcggcttcgg cctgctcctc
    15121 gccttcaccc cctgcgtctt cccgatgatc ccgatcctct ccggcatcat cgtcggccac
    15181 ggccacgcca tct
//
LOCUS       DAOQPA010000088        15070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2006_length_15070_cov_18.778688, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000088 DAOQPA010000000
VERSION     DAOQPA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2006_length_15070_cov_18.778688"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKX01_12215"
     CDS             complement(<1..544)
                     /locus_tag="PKX01_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ76939.1"
                     /translation="MSKHNDDSILSRRGFLKAGASMGGLAAMGTMLPMGGVWAAGSDK
                     PELEEVKIGFIPLTDCSSVVMASVMGFDKKYGIKITPSKEASWAGVRDKLANGELHAA
                     HVLYGLIYGLHLGIGGPKKDMAVLMTLNNNGQAITLSNALRDKGAVDGESLAKVVAKK
                     EKDYTFAQTFPTGTHAMWLYY"
     gene            complement(938..1513)
                     /locus_tag="PKX01_12220"
     CDS             complement(938..1513)
                     /locus_tag="PKX01_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HMZ76940.1"
                     /translation="MLRVLVVDSSRERAAEVCAALAVAGHQVTAVLSSALELSEQVLS
                     SKPDVIFIQADSPSRDTLEHLAIVNRDCPRPVLMCSSDSDTELIRRAVKAGVSSYVVD
                     SVSPERVDALMDVALAQFEAFQNLRAELDDAQRKLSERIVVDRAKGVLMKARGLTEED
                     AYHALRKLAMERGRKLADVAQDVLDMARLLM"
     gene            complement(1628..3172)
                     /locus_tag="PKX01_12225"
     CDS             complement(1628..3172)
                     /locus_tag="PKX01_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ76941.1"
                     /translation="MADKKLRVLVADDIASNRSIVGAFLARLNCQPIFAEDGHDAVEL
                     FRQEAPDVVLMDLMMPRMDGFEAIRQIRSFGDDHWVPIIILSALSGEGDIVKGLEAGA
                     DDYLTKPLSFPVFAARFKALGRLLDMQRRLSGSLEQFRAVANGVIDGIITADEQGVIL
                     SVNRSASLIFGYRASEMIGRNLKMLMPSPFRDEHDAYLEHYVLTGERRMIGQIREVIG
                     LRKDGSTFPLELGVSDLTLPGKRMFIGVVRDISESQRIRRKLADDAARLQRYHDEQEQ
                     EQELARSIMERQIRSDWLHDASVQYSVTPASNFSGDVVTVARSPQGVLYAMLADATGH
                     GLAAAVSVLPVLGAFYRLVAQGAPLASIVQELNSLLCGFSFRGRFVAAVLVCVDERAR
                     SGAVWVGGVPDALLVDGQGAVVRRFSSAHLPLGITALGRDEVVPEVFSWAEDSQLILC
                     SDGLTEAENAAGEQFGVRRLEDALRASTPAGRVEGVRHALADHLRGHPAGDDMSVLIV
                     DCEGKA"
     gene            complement(3275..4180)
                     /locus_tag="PKX01_12230"
     CDS             complement(3275..4180)
                     /locus_tag="PKX01_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ76942.1"
                     /translation="MSEPRILVVDDEPFNLEIVGEYLDGMGFDLVYAESGTEAWEVLT
                     RAGEHFDLILLDRMMPGLDGISVLRQVKADPSLRQIPVIMQTAATSPEQVREGLAAGA
                     YYYLTKPFEGEALQTIIRSALDDQRIRRDLTTDLLNHAQALALVAEAEFEARTLDDAR
                     KLAAFVALLGPQPEVLAMGLSELLVNGVEHGNLGIDFAEKSRLREEDRWEEEIARRLA
                     LPENTDKRVRLRVRRDDGRWVFEIRDDGPGFDWRRFLDFEPERAFAPNGRGIALSRQL
                     SFASLTYLPPGNQVVATVPIQDGTP"
     gene            complement(4181..7567)
                     /locus_tag="PKX01_12235"
     CDS             complement(4181..7567)
                     /locus_tag="PKX01_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ76943.1"
                     /translation="MMRVAAGRRWLLPVSVFAVGALITTAFWHNLRVHEAAAEQARHD
                     AVVERLAQQVEERLGKVDFILRGAAGLFGAQKLVERGEFETYVDSLHLHDELPEVVGV
                     GLARRVETKRMPAFEAVLGGLYGRPVRVYPQPDPAARALSFPITFFWPTGIVAARVVG
                     FDGYADPIRRRAMDLALQGERLAMTAAVKLAAFDQGESANAVTLYRLARAPEDASVVS
                     EAPGLVVLGLNLNVLFNHLFGAMGKAYGLEVEDVTDAAPLRLFYAPLAEADPAAPQAS
                     RQVSYGDRIWRLSVTPLAAGEQAGASGALALSGLATSLLAAFLMASVMGRRERAEDLA
                     LRMTDELRSSERRFELAVSATEEGIWEWRSDQRTLYLSSRCDRLLGYREGTLPRSARG
                     VLRNFTRASRRDLLLALRRHTKAPEVPLYCVLQVRRIDGSVGWFHVAGKAEFDAAGRH
                     VRTAGAASDVTELRRAQQAVTESHAKLDALYRNAYFGMAMVDGESRYLQVNPEFCRIV
                     GYSEEELREMSFYDLTPKEFLARDTRALVDLSLGVRSPPYEKEYIRKDGSRVPVVIAT
                     TYVGGSDKARWVVVEDATARKASQQAIQEAHATNESLISAMPDMLVQLDGQMRIVRYH
                     ADDRHSLSMPAESLLGKRVDEALPPEVAERFGRAALRASRSGEVQRVEYSTREGGASR
                     HFEARFRAVRTGGTLAVVRDVTDLKEAELALRESEARWQFALDGAGDGVWDWNLSTGK
                     VFRSTRWVSMLGYAPEDLDGSIDAWSSRVHPDDLPATLEAQAAHLRGETPIFTREFRL
                     RCKDGAYKWILARGLVVERDAAGRPLRMIGTQTDIDQVKAREAEILYHNTNLASLVEA
                     RTKDLMAAKEAAEAANEAKSIFLANMSHEIRTPMHGVLSYASLGESRIGAVSDDKLRS
                     YFNRIRLSGERLLLLLNDLLDLSKFEAGRMILNFEPTDMDTLVREVVREFDALLAARH
                     LSVKVQRDGVEPALFDGDAARVSQVIRNLLSNAAKFTPEGRAIHVHIRHDSLPEGRRA
                     SDLRRLPAVAVAVSDEGVGIPPEELDAVFEKFVQSSRTRTGAGGTGLGLAICREIVEA
                     HRGAISVCNNAAGGAEFTVILPVSRAAPRSAPSDKEREES"
     gene            complement(7834..8655)
                     /locus_tag="PKX01_12240"
     CDS             complement(7834..8655)
                     /locus_tag="PKX01_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ76944.1"
                     /translation="MTDRVWLIGDDAGVAERLDAWISDAGSPCERLTSREAVSRLTDG
                     GGPIKAAVVDAGDGHAVLALLEALGRRCKTTVLLVDAECYREIAAALAAGIGTFLRKP
                     VEPAALAAALTVEGRAERPGSGRFLFRTLDDVLDLSQLVAGLCPDPAAVEIGLSELML
                     NAVEHGNLGIGFERKGHLMNAGTWRQELERLLRLPENLVRFGYLDVELRGDGVRFKVL
                     DQGSGFDPQPYMNFNPERSVELHGRGIAIARMLAFPDLHFREEGSCVEGLVRRVC"
     gene            complement(8745..9113)
                     /locus_tag="PKX01_12245"
     CDS             complement(8745..9113)
                     /locus_tag="PKX01_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HMZ76945.1"
                     /translation="MAVAIVLFAHGARDAEWAEPARRVADAVQARAPAARVSLAFLEF
                     MAPTLAEAVEAAAADGATEVRVVPLFLARGGHLKRDVPALVEAARARHPGCRIELLPP
                     VGEDRGVVDAMAAYAIEAGH"
     gene            9353..10138
                     /locus_tag="PKX01_12250"
     CDS             9353..10138
                     /locus_tag="PKX01_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ76946.1"
                     /translation="MSLPAHAGLHPEAVPGGVVELPVAARGNARPEVRYDGHPVLLQR
                     RGEQWIAVVGIPLDAPVGEQHLDVGPADAPQAQPFPVHAKAYPVQKLTLKNPKMVNPD
                     EADLARIAAERDRQIEIRSRFRDVPVLRTDLDLPAVGRLSSRFGLRRVFNGEARSPHT
                     GLDVAVPAGTPVKAPADGVVSHVGDFYFNGRTVFVDHGQGFVSMVCHLTSASVEAGQA
                     VRRGEVLGLSGSSGRATGPHLHWSVYLNGAAVDPALFIPPPAR"
     gene            complement(10116..10913)
                     /locus_tag="PKX01_12255"
     CDS             complement(10116..10913)
                     /locus_tag="PKX01_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HMZ76947.1"
                     /translation="MTDAFSGGAEDPRRFGGIARLYGEAGRARLAAARVAVVGIGGVG
                     SWAAEALARSGVGALTLIDLDHVAESNLNRQIHATEATLGQAKVEAMAARIAGYAPGC
                     AVTRVDDFVTVENAATLLGAGFDYVVDAIDAVRVKVAMIAVCRAAGVPVVTCGAAGGQ
                     IDPTQIRVDDLARTIQDPLLAKVRGQLRKAHGFPRDGKKKFGVEAVFSTEPLRYPAPA
                     TVCDAAGGGAPQGPAGLNCAGFGSVVTVTASVGLFAAARALNGLAAG"
     gene            complement(10915..11868)
                     /locus_tag="PKX01_12260"
     CDS             complement(10915..11868)
                     /locus_tag="PKX01_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ76948.1"
                     /translation="MQGLTSYGHGIYAVDSGYTGTGIAAIHLIEEGGRAALVDTGNNA
                     ALVHVEEALKATGLKPEAVDYVLLTHIHLDHAGGAGAFMKAFPNAKLVVHPRGARHMA
                     EPSKLFAGVSAVYGPERAKELYGELIPVPLARIVEATDGLELSLAGRVIRVLDTPGHA
                     RHHVCYFDTGSRSFFTGDTFGVSYRHLDVDGRASIFPTTSPVQFDPVAMHASVARMEA
                     EQPVAMYLTHYAQLTEVPRLAADLHRLIDTHVAIAERHAGLPGGEPRQQAIEADLWAM
                     VADEARNAGWRLPEADWRRLLAVDVELNAQGLDVWLDSRSA"
     gene            12008..12271
                     /locus_tag="PKX01_12265"
     CDS             12008..12271
                     /locus_tag="PKX01_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76949.1"
                     /translation="MLTIQDCIELSDLTEDEVLAIAEHEHLPEMLAVEMGCYLCHTPD
                     GDKRIRRMIADDIRHAHALGDVQRTALLKSVLKHYVQEHHGNA"
     gene            12342..13370
                     /locus_tag="PKX01_12270"
     CDS             12342..13370
                     /locus_tag="PKX01_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76950.1"
                     /translation="MAILRCNHCGHVAETLVDANGQNMPCPRCITPAPVYDTVVFIRK
                     VLDQYFQLRDELNRLKAGASAMMQAEPARLATATATTAAPCDPPPVARPPAGARPNQF
                     ALNSQFAAIEQWFLRRQIQIRPPTDAPELSPAFERTAADLGRYHSLLRDFFERIPATP
                     TPDDNRLHLDLNARNAQEIGDLDAVFARLHGSAFLSHYTLDKPDRSLHAALPADPAIL
                     RFLSGEWLEWYSLGRLAEHFRKKGLRAAGARGLTALLADGDERPLDVFFLVNGNTPIC
                     LACRTGDVRPDIDTLLRLRKRLGIPRGQFIVCSPTLAEEQAEHLSTAHELSCVTPAGL
                     ITHIERLF"
     gene            13458..14462
                     /locus_tag="PKX01_12275"
     CDS             13458..14462
                     /locus_tag="PKX01_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76951.1"
                     /translation="MALLRCNQCGHVAEPAADTVGQHLPCPHCGTSVPVYDTVLFVRK
                     VLQQYFALRDENTRLKDALAAAPATTEEAPAAPASAPPLSDIDLHNSSHFASEAQHAA
                     IQQWFARRQIEARPNRDAVDTTGFYDEIAVALGDHYELYRDVLDKIRWGHKKDIPQFS
                     LNLTERSQKDGQALNAFCRQLHDSAFLSKYFYQKQDKIIRASVQTAPAIRAFFAGEWL
                     EWYALMKLLAFFRDRGLGFSCTRNLHITFANEDLHELDVFFLLDDGTPVCVECKTGEF
                     RADIDKMLRLRKRLGIDRSQFIVCALGLSDEQAAGLSGTYELSFVTPAGLLPHLARLF
                     "
     gene            14497..>15070
                     /locus_tag="PKX01_12280"
     CDS             14497..>15070
                     /locus_tag="PKX01_12280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HMZ76952.1"
                     /translation="MTPRARELLSHVFGYSGFRGPQEAIVEHVAAGGDALVLMPTGGG
                     KSLCYQIPALLRPGVAIVVSPLIALMQDQVSALKEVGVAADFLNSSLAFEQAMEIERA
                     LLAGALDLLYVAPERLVTPRFLDLLDHLREAGQLALFAIDEAHCVSQWGHDFRPEYLQ
                     LSLLPERYPHIPRIALTATADAQTRREIAER"
BASE COUNT         2554 a         5748 c         4486 g         2282 t
ORIGIN      
        1 agtagtagag ccacatggcg tgggtgccgg tggggaaggt ctgggcgaag gtgtaatcct
       61 tctccttctt cgccacgacc ttggcgaggg attcgccatc caccgcgccc ttgtcgcgca
      121 gcgcgttgga cagggtgatc gcctgaccat tgttgttgag ggtcatcagc acggccatgt
      181 ccttcttggg accgccgatg cccaggtgca ggccatagat caggccgtac agcacgtggg
      241 ccgcgtgcag ctcgccattg gcgagcttgt cgcgcacacc ggcccaggat gcttccttgc
      301 tcggcgtgat cttgatgccg tatttcttgt caaagcccat caccgaggcc atgaccacgg
      361 aagaacagtc ggtgagcggg atgaacccga tcttcacttc ttccagttcc ggcttgtccg
      421 agccggctgc ccaaacgccg cccatcggaa gcatcgtacc cattgctgcc agtcctccca
      481 tgcttgcccc ggccttgaga aagccgcggc gggaaagaat cgaatcgtcg ttgtgcttgc
      541 tcatagctcg cccctgaata caaaaaaaac gggcgtcaca tcgccccgcg gatggcatgc
      601 gggggatgga cgcccttgtc caaagtctga tcgcctgatc aagccggacc gccgttggac
      661 cgacttcatt caagacttag cgagaggcgt gccagaaata tcaagtcatt gatttacatc
      721 aaaataacaa aacagcgaaa atttcaaacc agacctaccc ctaaagtggt agccggaaag
      781 tgggtcaaca gcgcactgct gcggtgcaaa acgactggct ttcgcactgt tccggaccat
      841 gcacgcccat tcaccggcgt ctcaaaacag acagggcagg gtcgcctgca cgcgccctgc
      901 cctgctccca gcctgtccga tgcccctgcc ggagtggtca catcagcagg cgggccatgt
      961 cgagtacgtc ctgggccacg tccgcgagct tgcgcccgcg ctccatcgcg agcttgcgca
     1021 gggcatgata ggcgtcctct tcggtgagcc cgcgcgcctt catcagcacc cccttggcac
     1081 ggtccaccac gatgcgttcc gacagtttgc gctgggcgtc atccagctcg gcgcgcaggt
     1141 tctggaaggc ctcgaactgg gccagcgcca cgtccatcaa cgcatccacc cgctccgggc
     1201 tcacagaatc aacgacatag gacgacacgc cggccttcac cgcgcgccgg atcagttcgg
     1261 tatccgaatc cgaagagcac atcagcaccg gccgcggaca gtcacgattg acgatcgcca
     1321 gatgttcaag ggtgtcgcgg ctcggggaat cggcctggat gaagatgacg tccggcttcg
     1381 agctcaacac ctgctccgaa agctccaggg cgctcgacag gaccgccgtc acctgatggc
     1441 cagccaccgc cagggccgca cagacttcgg cggcccgctc acgggaagaa tcgacgacga
     1501 ggacacgcag catgggatgg tccggacaac gcaatcagca gccagaattc agcaggaacc
     1561 gtgccaaatc aggcaccggc gcaaggatcg gacgatcagc cgccggtgtc caggtccgct
     1621 cccgtgctca ggcctttccc tcgcaatcga cgatcagcac cgacatgtcg tcgccggccg
     1681 gatggccacg cagatgatct gcgagggcat gacggacccc ctcgacccgc ccggcgggcg
     1741 tcgacgcccg caaggcgtcc tcgaggcgac ggacgccgaa ctgctcaccg gcggcattct
     1801 ccgcttcggt gagaccatcc gaacacagga tgagctggct gtcctccgcc cagctgaaga
     1861 cctccggcac cacctcgtcc cgccccaggg cagtgatccc cagggggaga tgagccgacg
     1921 agaatcgccg gaccacggcg ccctgaccat ccaccagcag ggcatcgggc acgccaccga
     1981 cccacaccgc gcccgagcgg gcgcgctcat ccacgcacac cagcaccgca gcgacaaagc
     2041 gcccacggaa ggagaagcca cacagcaggc tgttcagttc ctgcacgatg gacgccagcg
     2101 gcgcgccctg ggccaccagc cggtaaaagg cgcccagcac cggcagcacg ctcaccgcgg
     2161 ccgccaggcc atggccggtg gcatcggcca gcatggcata caggacgccc tgcggcgaac
     2221 gggccaccgt caccacatcg ccggagaagt tgctcgccgg cgtcaccgag tactggaccg
     2281 acgcatcgtg caaccagtcg ctgcggatct gccgctccat gatgctccgg gcgagctcct
     2341 gctcctgttc ctgctcgtcg tggtagcgct gcaggcgggc cgcgtcgtcg gccagcttcc
     2401 ggcggatgcg ctgagactcg gagatgtccc gcacgacccc gatgaacatg cgcttgcccg
     2461 gcagggtcag gtccgacacc cccagctcca ggggaaacgt cgagccgtcc ttgcgcagac
     2521 cgatcacctc gcggatctgt ccgatcatgc ggcgctctcc ggtgaggacg tagtgctcca
     2581 ggtaagcgtc gtgctcgtcg cggaagggcg agggcatcag catcttgagg ttgcggccga
     2641 tcatctcgct ggcgcggtag ccgaagatca ggctcgccga gcggttcacc gacaggatga
     2701 ccccctgctc gtcggccgtg atgatgccgt cgatcacgcc attggccacc gcccgaaact
     2761 gttccagcga accggacagg cgccgctgca tgtccagcag gcgacccagg gccttgaagc
     2821 gggccgcgaa gacggggaag gacagcggct tggtcaggta gtcatcggcg cccgcctcca
     2881 gccccttgac gatgtctccc tcgccgctca gggccgacag gatgatgatg ggcacccagt
     2941 ggtcgtcccc gaaggagcgg atctggcgga tcgcctcgaa gccgtccatg cgcggcatca
     3001 tcaggtccat cagcaccacg tccggggcct cctgacggaa gagctcgacc gcgtcgtgac
     3061 cgtcctctgc aaagatcggc tggcagttca gccgtgccag gaaggcgccc acgatggagc
     3121 ggttcgaggc aatgtcgtcg gcaaccagca cgcgaagctt cttatcggcc atggcgtaca
     3181 tttccctgtt gagtcggatt caaggcgaga gcgggatccc cgccggcgca cacgatgctg
     3241 cctggcccgc cggaggcatg ggacacgcgg ccgctcacgg cgttccatcc tgaatcggaa
     3301 cggtggccac cacctgattg cccggcggca gataggtgag gctcgcgaac gacagctgac
     3361 gcgacagcgc gatgccgcgc ccattcggcg caaaggcccg ctccggctcg aaatccagga
     3421 agcgccgcca gtcgaatccc ggcccgtcgt cacggatctc gaagacccag cgcccgtcgt
     3481 cccggcgcac ccgcaggcgc acgcgcttgt cggtattttc cggcagcgcc agacggcggg
     3541 cgatctcctc ttcccagcgg tcctcttccc gcaggcggct cttttcggcg aagtcgatgc
     3601 ccagattgcc atgctccacg ccattgacaa gcagctccga caggcccatc gccagcactt
     3661 ccggctgggg cccgagaagc gccacgaagg ccgccagctt gcgcgcatcg tccagggtgc
     3721 gcgcctcgaa ttcggcctcc gccaccagcg ccagggcctg ggcgtgattg agcaggtcgg
     3781 tggtcagatc ccggcggatg cgctgatcgt cgagggccga gcggatgatg gtctgcaggg
     3841 cctcgccttc aaaaggcttc gtgaggtaat agtaggcccc cgccgccagc ccctcgcgca
     3901 cctgttccgg tgacgtggcc gccgtctgca tgatcaccgg gatctgccgc aagctcgggt
     3961 ccgccttgac ctggcgcagc accgagatgc cgtccagtcc gggcatcatg cgatccagca
     4021 ggatcaggtc gaagtgctcg cccgcacggg tcagcacctc ccaggcttcg gtgccggact
     4081 cggcatagac cagatcgaag cccatgccgt ccaggtactc cccgacgatc tccagattga
     4141 agggctcgtc gtccacgacg agaatcctcg gctcgctcat tcaactctcc tctcgttcct
     4201 tgtccgacgg ggcgctgcgc ggggccgcgc ggctgaccgg caggatcacc gtgaattcgg
     4261 ccccgccggc cgcgttgttg cacacggaga tcgcgccgcg atgggcctcc acgatctccc
     4321 ggcagatggc cagcccgagc ccggtcccac cggcgccggt gcgggtacgg ctgctctgca
     4381 cgaatttttc aaagacggcg tccagttcct cgggcggaat gcccacgccc tcgtcggaga
     4441 ccgccaccgc caccgcgggc aggcgccgca agtccgacgc acgtcgccct tcgggcaggc
     4501 tgtcgtggcg gatgtgaacg tggatggcgc gcccttccgg tgtgaacttg gccgcgttgg
     4561 acagcaggtt gcggatcacc tgggagaccc gcgccgcgtc gccgtcgaaa agggccggct
     4621 cgaccccgtc ccgctgaacc ttcacgctca ggtgccgggc cgccagcagg gcgtcgaact
     4681 cgcgcacgac ttcccggacc agggtgtcca tgtccgtcgg ttcaaaattg aggatcatcc
     4741 ggccggcttc gaacttggac agatcgagca ggtcgttgag cagcagcagc agacgctcgc
     4801 cggacaggcg gatgcggttg aagtaggaac gcagcttgtc gtcgctcacc gcaccgatgc
     4861 gcgactctcc gagactggcg tagctcagca ccccgtgcat gggggtgcgg atctcgtggg
     4921 acatgttggc caggaagatg gacttggcct cgttggccgc ctccgcggct tccttcgcgg
     4981 ccatcaggtc cttggtccgc gcctccacca gactggccag attggtgttg tggtacagga
     5041 tctccgcctc ccgcgccttc acctggtcga tatccgtctg ggtgccgatc atgcgcagcg
     5101 gccggcccgc cgcgtcccgc tcgacgacca gtccccgggc gaggatccat ttgtacgccc
     5161 catccttgca gcgcaagcgg aattcccgtg tgaagatcgg tgtctcgccg cgcaggtgcg
     5221 ccgcctgggc ctccagcgtg gccggcaggt cgtccggatg gacgcggctc gaccaggcgt
     5281 cgatggaccc atccagatcc tcgggcgcgt agcccagcat gctcacccag cgggtcgaac
     5341 ggaacacctt gccagtggac aggttccagt cccacacccc gtcgccggca ccatccaggg
     5401 cgaactgcca gcgcgcctcg ctttcccgca gggccagctc ggcttccttc agatcagtca
     5461 catcccgcac caccgccagc gtaccgccgg tccgtaccgc ccggaaacgc gcctcgaagt
     5521 ggcggctggc gcccccttcg cgggtggaat actccacgcg ctgaacctcg ccgctgcgcg
     5581 acgcacgcag tgcggccctg ccgaaacgct ccgccacctc cggcggcaga gcctcatcca
     5641 cccgtttgcc gagcagggac tctgccggca tgctcaggct gtgacggtcg tccgcgtgat
     5701 agcgcacgat gcgcatctgg ccgtccagtt gcaccagcat gtcgggcatg gccgagatca
     5761 gcgactcgtt ggtggcgtgc gcctcctgga tcgcctgctg gctggccttg cgggcggtcg
     5821 catcctccac caccacccag cgcgccttgt cgctgccgcc cacgtaagtg gtggcgatga
     5881 ccaccggcac gcgggaaccg tccttgcgga tgtattcctt ctcgtagggc ggcgagcgga
     5941 cgcccaggga caggtccacc agcgcgcggg tgtcccgcgc caggaactcc ttcggcgtca
     6001 ggtcatagaa gctcatctcc cgcagctctt cctcggaata cccgacgatg cggcagaact
     6061 ccggattcac ctggagatac cgggactccc cgtccaccat ggccatgccg aaataggcat
     6121 tgcggtacag ggcatccagc ttggcgtggg attccgtgac ggcctgctgg gcgcggcgca
     6181 gttcggtgac atcggacgca gccccggccg tgcgcacatg ccgccccgcg gcgtcgaact
     6241 cggccttgcc ggccacgtgg aaccagccca cgctgccgtc gatgcgccgc acctgcagca
     6301 cacagtagag gggcacctcc ggcgccttgg tgtggcggcg cagggccagc agcaggtcgc
     6361 ggcgcgacgc ccgcgtgaag ttgcgcaaca ccccgcgcgc cgagcgcggc aaggtgccct
     6421 cgcgataccc cagcaggcgg tcgcaacgag acgacagata cagcgtgcgc tgatccgaac
     6481 gccattccca gatgccttcc tcggtggccg acaccgccag ctcgaaccgc cgttcgctac
     6541 tgcgcagctc atcggtcatg cgcagcgcaa gatcctcggc gcgctcacgc cgccccatca
     6601 cgctggccat caggaaagcc gccagcagac tggtcgccaa ccccgacaga gccagcgcac
     6661 cggacgcgcc ggcctgctca ccggccgcca gcggcgtgac gctcaggcgc cagatccggt
     6721 cgccgtagct cacctgacgg ctcgcctgcg gcgcagccgg atccgcctcc gcgagcggcg
     6781 cgtagaacaa gcgcaggggc gccgcatcgg tgacgtcctc cacctccagg ccgtacgcct
     6841 tccccatcgc cccgaagaga tgattgaaca ggacgttcag attgagccca agcaccacca
     6901 gccccggcgc ctcggacacc acgctggcat cttccggagc ccgcgccagg cggtacaggg
     6961 tgaccgcatt ggccgactcg ccctgatcga atgccgccag cttcacggcg gccgtcatgg
     7021 ccagtcgctc gccctgcagc gcgagatcca tcgcgcgacg gcggatcggg tccgcgtagc
     7081 cgtcaaagcc caccacgcgc gcggcgacga ttcccgtggg ccagaagaag gtgatcggaa
     7141 agctcagcgc gcgggcagcg gggtctggct gcggatagac ccgcacgggc cgcccgtaga
     7201 gtcccccgag gacggcctcg aaggccggca tgcgcttcgt ctccacccgc cgggcgagcc
     7261 cgacccccac cacctcgggc agctcgtcat gcaggtgcag gctatccacg taggtttcga
     7321 actcgccccg ctccaccagc ttctgggcgc cgaacagccc tgcggccccg cgcaggatga
     7381 aatccacctt gccgaggcgc tcctccacct gctgggccag ccgctccacc accgcgtcat
     7441 ggcgggcctg ctcggccgcc gcctcatgga cgcgcaggtt gtgccagaac gccgtggtga
     7501 tgagcgcccc caccgcaaac accgacaccg gcagcagcca tcgtctgccg gcggcgacac
     7561 gcatcatcgg gaagggagga ggaatccggg gcataacgcc ctgtgaacgg cgtgtcgcct
     7621 caaaccttga gccgcatcgc cccccttccg gccggcggtt caggcgccgg cgcgtggggt
     7681 cggccgtctc ggccaaggca gcgcattgtc atcggattgg cacacgcggg cgtcacgcaa
     7741 agccgctgcg gggcggcacc ccggtctggc cggtcaatct gccggattcc cgctcagggc
     7801 gcccggagcc cgcgtccgcc tgcagcgccg gactcagcag acgcgacgca ccagtccttc
     7861 gacgcaggac ccttcctcgc ggaaatgcag gtccgggaag gccagcatgc gcgcgatggc
     7921 gatgccacgt ccgtgcaatt ccaccgagcg ctccggattg aagttcatgt acggctgcgg
     7981 atcgaagccg ctgccctggt cgagcacctt gaagcgcacg ccgtcgcccc gcagttccac
     8041 gtccagatag ccgaagcgga ccagattctc cggcaggcgc aggagtcgct ccagctcctg
     8101 ccgccaggtg ccggcgttca tgaggtgccc cttgcgctcg aagccgatgc ccagattgcc
     8161 gtgttccacg gcgttgagca tcagctcgga gagcccgatc tccaccgccg ccggatcggg
     8221 gcacagaccg gccaccagct gcgacaggtc gagcacatcg tccagcgtgc ggaacaggaa
     8281 gcgtccgctg cccggccgct cggcgcgccc ctcgaccgtc aatgccgccg ccagcgccgc
     8341 cggctccacc ggcttgcgca ggaaggtccc gatcccggcg gccagcgccg cggcgatctc
     8401 ccggtaacat tccgcatcca ccagcagcac cgtcgtcttg cagcgacgcc cgagcgcctc
     8461 gagcagcgcc agaacggcat gcccgtctcc cgcgtccacc acggcggcct tgatcgggcc
     8521 gccgccatcg gtcagccgcg agacggcttc gcgggacgtc aggcgttcgc atggactgcc
     8581 cgcgtcgctg atccaggcgt ccagcctctc tgccacgccc gcatcgtcac caatcagcca
     8641 cactcggtcc gtcatcgaat caagctcctc caccggtcgc ctttgaccgg cctctttcca
     8701 tccggtccgt cccgccttcg ggcggtcgga ccgtgctcac atcctcagtg tccggcctcg
     8761 atggcgtacg ccgccatggc atccaccacc ccccggtcct cacccaccgg cggcaacagc
     8821 tcgatccggc agcccgggtg acgcgcccgt gccgcctcca ccagcgccgg cacatcccgc
     8881 ttcagatgcc caccgcgggc caggaacagg ggcaccacgc gcacttccgt ggccccgtct
     8941 gctgccgccg cctcgaccgc ctcggccagg gtcggcgcca tgaactccag aaaggccaga
     9001 ctcacccgtg cagcaggcgc ccgcgcctgc accgcatcgg ccacccggcg tgccggttcg
     9061 gcccattccg catcccgcgc cccgtgggca aacagcacga tcgccaccgc cattcaacac
     9121 tccgtattcc cttggcccgc cagccatgcc cgagccgtgc aagaatcggc tccgcaaggc
     9181 tccgcgcccc attcggcgcg tgcgcatgcc aagaataatg ttaatccctg aaaatcaagc
     9241 caggccccat tttgcccacg cggcataaga accttcagcc atgtgtgaaa tattgcccat
     9301 ttatcggcaa cttgtccttc gtctgcttgt cggcgtcacc gccggcgcgg gcgtgagtct
     9361 gcccgcccac gccggactgc atcccgaagc cgtccccggc ggcgttgtcg aactccccgt
     9421 cgccgcccgc ggcaacgccc gccccgaggt ccgctacgac ggtcacccgg tcctcctcca
     9481 gcgccgtggc gaacagtgga tcgccgtggt gggcatcccc ctcgacgcgc ccgtcggcga
     9541 acagcatctg gacgtaggcc ccgccgacgc gcctcaggcg cagcccttcc cggtgcatgc
     9601 caaagcctac ccggtgcaga agctcacgct caagaacccc aaaatggtca atccggacga
     9661 agccgacctt gcccgcatcg ccgccgaacg ggaccgccag atcgagatcc gcagccgctt
     9721 ccgcgacgtc cccgtgctgc gcaccgacct ggacctgccc gccgtcggac gcctctccag
     9781 ccgcttcggg ctgcgccgtg tcttcaatgg cgaagcccgc tcgccccaca ccgggctgga
     9841 cgtggcggtg cccgccggca cgccggtcaa ggcgccagcc gacggcgtgg tgagccatgt
     9901 gggggatttc tacttcaacg gccgcacggt cttcgtcgat cacggccagg gcttcgtcag
     9961 catggtctgt cacctcacca gcgccagcgt cgaggccggc caggccgtcc gccgcggcga
    10021 agtgctgggc ctctccggca gcagcggccg ggccaccggg ccgcatctgc actggagcgt
    10081 ctacctcaac ggcgcggcgg tcgatcccgc cctgttcatc ccgccgccag cccgttgagc
    10141 gcccgcgccg cagcaaacag cccgacgctg gccgtcactg tcaccaccga cccgaagccc
    10201 gcgcaattga gtcctgccgg cccctgcggc gcgccgccgc ccgcggcatc gcagaccgtc
    10261 gccggtgccg gatagcgcag gggctccgtc gagaacaccg cctccacgcc gaacttcttc
    10321 ttcccatcgc ggggaaagcc gtgggccttg cgcaactgcc cgcgcacctt ggccagcagc
    10381 gggtcctgga tcgtgcgcgc caggtcgtcc acgcggatct gcgtcgggtc gatctgcccg
    10441 cccgccgcgc cgcaggtcac caccggcacg cccgccgcgc ggcacacggc gatcatcgcc
    10501 accttcacgc gcaccgcgtc gatggcatcc accacgtaat cgaacccggc accgagcagg
    10561 gtcgccgcgt tctccaccgt cacgaaatca tccacccgcg tcaccgcgca acccggcgca
    10621 tagccggcga tgcgcgcggc catcgcctcc accttggcct ggccgagggt cgcctcggtc
    10681 gcgtggatct gccggttgag attggactcc gccacgtggt ccagatcgat cagcgtgagc
    10741 gcgcccacgc cgctgcgggc cagcgcctct gccgcccagg aacccacgcc gccgatgccc
    10801 accaccgcca cccgcgccgc cgccagacgg gcccgcccag cctcgccgta gaggcgcgcg
    10861 atgccaccga agcggcgcgg atcctccgcg ccccctgaaa acgcgtccgt catctcaggc
    10921 gctgcgggaa tcgagccaca catccaggcc ctgggcgttc aactccacgt ccaccgccag
    10981 cagacggcgc cagtcggctt ccggcagacg ccagccggcg ttgcgggcct cgtccgccac
    11041 catcgcccac aggtccgcct cgatggcctg ctggcgcggc tcgccaccgg gcaggccggc
    11101 atgacgctcg gcgatggcca catgggtgtc gatcaggcgg tgcagatccg ccgccaggcg
    11161 cggcacctcg gtgagctggg cgtaatgggt cagatacatg gccaccggct gctccgcctc
    11221 catgcgcgca acggacgcat gcatcgccac cggatcgaac tgcaccggcg aggtggtcgg
    11281 gaagatgctc gcccggccgt ccacgtccag gtggcggtag gacacaccaa aagtatcgcc
    11341 ggtgaagaag ctgcggctgc cggtgtcgaa gtaacacacg tgatggcgcg catggcccgg
    11401 cgtatccagc acgcggatca cccgcccggc gagggacagc tcgagcccgt cggtggcttc
    11461 cacgatgcgc gccagcggca ccgggatcaa ctcgccgtag agctccttgg cccgctccgg
    11521 cccatacacc gccgacaccc ccgcaaacag cttggacggc tccgccatgt gacgcgcacc
    11581 gcggggatgc accaccagct tcgcgttcgg gaacgccttc atgaaggccc ccgcgccgcc
    11641 cgcatgatcc aggtggatgt gggtcagcag cacgtaatcc accgcctccg gcttcaggcc
    11701 cgtggccttc agcgcctctt ccacatgcac cagcgccgca ttgttacccg tatccaccag
    11761 cgccgcccgg ccgccttcct cgatgaggtg gatcgccgcg atccccgtgc ccgtgtaacc
    11821 cgaatccaca gcgtagatgc cgtgcccgta ggaagtcagc ccctgcatgc tcatcccttt
    11881 gaaaaaagaa cggattctag cccgcccctg tatgaatgcg accgttgcgt tgcatcaatg
    11941 tttcagtcgc ccgaaaggcg tccaatggac ttaaaaagca ttatcacaac acaaggaggg
    12001 aagcatcatg ttgacgatcc aggactgcat cgagctatcc gatctcaccg aggacgaagt
    12061 cctcgccatc gccgagcacg aacacctgcc ggagatgctg gccgtggaaa tgggctgcta
    12121 tctctgccac accccggacg gcgacaagcg catccgccgc atgatcgccg acgacatccg
    12181 ccacgcccac gccctgggcg atgtgcagcg caccgcgctc ctcaaatccg tgctcaagca
    12241 ctacgtgcag gagcatcacg gcaacgcctg agcgttcatc cagtcgccac ggagatatcg
    12301 ggttggcata atccgccaac ccgaccgatc atgacatccc catggcaatc cttcgatgca
    12361 atcattgcgg ccacgtcgcc gagacccttg tcgacgccaa cggccagaac atgccctgcc
    12421 cccgctgcat cacgccggcg ccggtctacg acaccgtcgt cttcatccgc aaggtgctcg
    12481 accagtattt ccagctgcgg gacgagctca accggctcaa ggcaggcgct tccgccatga
    12541 tgcaggcgga gccggcccgc ctcgccaccg ccacggcgac gacggcagcg ccgtgcgatc
    12601 cgcctcccgt cgcccgcccg ccagccggcg cccgacccaa ccagttcgcc ctcaacagtc
    12661 agttcgccgc catcgagcaa tggttcctgc gtcgtcagat ccagatccgc ccccccaccg
    12721 acgcgcccga actcagcccc gccttcgaac gcacagccgc cgacctgggc cgataccact
    12781 ccctgctgcg cgacttcttt gaacgcatcc ccgccacgcc caccccggac gacaaccgcc
    12841 tccacctcga cctgaacgcc cgcaacgccc aggagatcgg tgacctggac gccgtcttcg
    12901 cgcgcctgca cggcagcgcc ttcctctccc attacaccct cgacaagccc gaccgcagtc
    12961 tccacgccgc cttgcccgcc gaccccgcca tcctgcgctt cctctcgggc gagtggctgg
    13021 agtggtacag cctcggccgc ctcgccgagc acttccgcaa aaaaggcctg cgcgccgccg
    13081 gcgcccgcgg gctgaccgca ctcctggccg acggcgacga acggcccctt gacgtcttct
    13141 tcctcgtcaa tggcaacacg cccatctgcc tcgcctgccg caccggcgac gtccgcccgg
    13201 acatcgacac cctcctgcgt ctgcgcaagc gcctcggcat cccccgcggc cagttcatcg
    13261 tctgcagccc caccctcgcc gaggagcagg ccgagcacct cagcaccgcc cacgaactca
    13321 gctgcgtcac cccggccggc ctcatcacgc acatcgaacg cctcttctga aaccggcgtg
    13381 cgggcgccgc cggtcgccag cccgccacca gattggcata atcgggcctt tccaccgagt
    13441 cccacgccga ttccgccatg gccctcctcc gttgcaacca gtgcggccac gtcgccgagc
    13501 ccgccgccga caccgtcggc cagcatctgc cctgccccca ctgcggcacc agcgtcccgg
    13561 tctacgacac cgtcctcttc gtgcgcaagg tgctccagca atacttcgcc ctccgcgacg
    13621 agaacacccg cctgaaggac gccctcgccg ctgcgccggc cacgacggag gaagcgcccg
    13681 ccgcccccgc cagcgccccg cccctctccg acatcgacct gcacaactcc agccacttcg
    13741 ccagcgaagc ccagcacgcc gccatccagc agtggttcgc ccggcgccag atcgaggccc
    13801 gccccaaccg ggacgccgtc gacaccaccg gcttctacga cgagatcgcc gttgccctcg
    13861 gcgatcacta cgagctctac agggacgtcc tcgacaagat ccgctggggc cacaaaaagg
    13921 acatccccca gttcagcctc aacctcaccg agcgcagcca gaaggacggc caggccctca
    13981 atgccttctg ccgccagctc cacgacagcg ccttcctgtc caagtacttc taccagaagc
    14041 aggacaagat catccgcgcc tccgtccaga ccgcccccgc catccgcgcc ttctttgccg
    14101 gcgaatggct ggagtggtac gccctgatga agctcctcgc ctttttccgc gaccgtggcc
    14161 tgggcttctc ctgcacccgc aaccttcaca tcaccttcgc caacgaagac ctgcacgaac
    14221 tcgacgtctt cttcctgctg gacgacggca cgccggtctg cgtcgaatgc aagactggcg
    14281 aattccgcgc cgacatcgac aagatgctgc gcctgcgcaa gcgcctcggc atcgaccgca
    14341 gccagttcat cgtctgcgcc ctcggcctca gcgacgaaca ggccgccggc ctcagcggca
    14401 cctacgagct cagcttcgtc acccccgccg gcctcctccc ccatctcgcc cgactcttct
    14461 gacgcccccc gcggaccggg gtaaaatccg gtccccatga ccccccgcgc ccgcgaactc
    14521 ctctcccacg tcttcggcta cagcggcttc cgcggccccc aggaagccat cgtcgagcac
    14581 gtggccgcgg gcggcgacgc cctcgtgttg atgcccaccg gcggcggcaa gtccctctgc
    14641 taccagatcc ccgccctgct gcggcccggc gtcgccatcg tcgtgtcgcc cctcatcgcc
    14701 ctcatgcagg accaggtcag cgccctcaag gaagtgggcg tcgccgccga tttcctcaac
    14761 tccagcctcg ccttcgagca ggccatggag atcgagcgcg ccctcctcgc cggcgccctc
    14821 gacctgctct acgtcgcccc ggaacgcctc gtcaccccgc gcttcctcga cctcctcgat
    14881 cacctgcgcg aagccggcca gctcgccctc ttcgccatcg acgaagccca ctgcgtgtcc
    14941 cagtggggcc acgacttccg gcccgaatac ctgcagctct ccctgctccc cgagcgctac
    15001 ccccacatcc cgcgcatcgc cctcaccgcc accgccgacg cccagacccg ccgcgagatc
    15061 gccgagcgcc
//
LOCUS       DAOQPA010000089        14881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2042_length_14881_cov_18.495616, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000089 DAOQPA010000000
VERSION     DAOQPA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14881
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_14881_cov_18.495616"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKX01_12285"
     CDS             complement(<1..446)
                     /locus_tag="PKX01_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HMZ76953.1"
                     /translation="MQLFALGLNHHTAPLAIREKVAFRPERLVEALGDFVQRKPVKEA
                     AILSTCNRTEMYFATGEPQSAADWFADYHRVALQELSPYLYTYPQRDAVRHVFRVASG
                     LDSMVLGEPQILGQMKDAVRQAEEAGTLGATMHKLFQRTFSVAKEVR"
     gene            complement(492..1469)
                     /gene="gcvA"
                     /locus_tag="PKX01_12290"
     CDS             complement(492..1469)
                     /gene="gcvA"
                     /locus_tag="PKX01_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HMZ76954.1"
                     /translation="MAYRLPPLSSLRAFEAAARHLSFALAARELHVTPAAVSHQIKAL
                     ESYLGVALFERLPRAVVLTAQGRALLPRVQEGFECFAAAIEAAREAGEGGLTVHAPPS
                     FAQRWLVPRLAGFAAAHPEIALRVASDGANIDGVGAPPEDRLADLAAEPDVLAIRFGN
                     GRYPGFRVERVLTPDYIVVGRPDLVAASALRQPADLLAHPLIHDESIPDASVRPSWAE
                     WFRRVGVADADVSRGPRFSSAVLAVEAALAGQGVVLTLAPLVAADIAAGRLRQPFPER
                     LASPYAYYMVTAEAQAERRPVRAFRDWLRAELARTSGAVEGAGSAEKRG"
     gene            1646..1792
                     /locus_tag="PKX01_12295"
     CDS             1646..1792
                     /locus_tag="PKX01_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76955.1"
                     /translation="MKLDSLYDHLTAELDHRVEVERHSLSIAAHEAGEYEAYFKEVFG
                     GSVF"
     assembly_gap    1818..1917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2147..3028
                     /locus_tag="PKX01_12300"
     CDS             2147..3028
                     /locus_tag="PKX01_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ76956.1"
                     /translation="MISVPFPRRVLLRAAAATLLAAAAGLPCAAFAEDVLRVSAIPDE
                     APTELQRKFAPLGAYLEQETGMKVSFVPVTDYAAVVEALATRKIDMAWLGGFTFVQAK
                     LRTHGTAVPLVQRAEDAKFTSKFITAQDGIKSLADLKGKTFAFGAPSSTSGHLMPRYF
                     LGQEGLNPDKDFKTVAFSGAHDATVAFVQAGKVDAGVLNASVWDKLLEQKKVDTAKVR
                     VFATTPPFFDYNWTVRGDLDPKLQARITQAFLKLDPANPAHKEIMALQRASKFIPTKA
                     ENYAGIETAAHAAGLLK"
     gene            3081..3878
                     /locus_tag="PKX01_12305"
     CDS             3081..3878
                     /locus_tag="PKX01_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ76957.1"
                     /translation="MGFSLKGVGLVHANGHRALQGIDLSIADGERVALVGASGAGKTT
                     LLRLLAAARRPTEGCLHLLGADPWSASPAALRRLRARIGLVHQSPPIPPRQRVVTAVL
                     AGRLGHWPLWKALASLVLPADAPGAARELARLDLTDKLYERCDRLSGGQLQRVALARV
                     LYQQPDLFLADEPVSALDPALSELTVALLNEEATRRGVTLVASLHAVDLALRHFPRVV
                     GLQDGRILFDLPSREVSAAQLRTLYAAEGGLPVASICPAGQPSRRCS"
     gene            3994..4815
                     /locus_tag="PKX01_12310"
     CDS             3994..4815
                     /locus_tag="PKX01_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphonate ABC transporter permease"
                     /protein_id="HMZ76958.1"
                     /translation="MSPTPRDPAALPRLGATVLALALLGPLLHLTDFRPAALFEAGNL
                     RTVGQFLGGFLPPARDPDFLALLARATVETLAMATAGMAVAFLLALPLGLAMARSLSV
                     SRLADARGSRTGALLRALVRGLLTLLRGIPELVWALLFVRVFGLGPAAGVLALGITYG
                     GMLGKVYGEILESTDARPARGLLEAGSGRLAAFSYGLLPLAAPELVSYTVYRWECAVR
                     ASVVMGFVGAGGLGQLMDQSMKMLNGGEAATILASFLLLVVAADALSEWLRSRLA"
     gene            4812..5606
                     /gene="phnE"
                     /locus_tag="PKX01_12315"
     CDS             4812..5606
                     /gene="phnE"
                     /locus_tag="PKX01_12315"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HMZ76959.1"
                     /translation="MNVRTPSSPRVAGVTPGGLLAGLAVLAAVAGSFVFLDLPLTAVF
                     TGAAATEALDFAGRFLPPETDTDFMAKVGGAALETLAISLVATLLAAAGGALLALPAS
                     GRHGRPARIVARFVLNLLRSVPELVWATLLVLAAGLGPFAGTLALAVHTTGVLGRLFA
                     EALENAPAAPAEALTGGGAPALASFLYGTLPQVGDQWLAYALYRWEMNIRMAAILGFV
                     GAGGLGGLLYYSLSLFREAEAGTVILAMLVMAIAVDSVSAWLRRAR"
     gene            complement(5664..6173)
                     /locus_tag="PKX01_12320"
     CDS             complement(5664..6173)
                     /locus_tag="PKX01_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76960.1"
                     /translation="MDALGGDVHEQERVVHDAGGRVSDLSRQAADAAGSARDLARASN
                     RIQSVVEVIQQIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEKSRTSAGEI
                     GADISALAGEIGRVALQIEQQAGDVTRLSSLLTDIAGISSRTAGNADHTRNVADRLRQ
                     LTEAQLNQH"
     gene            complement(6546..7694)
                     /locus_tag="PKX01_12325"
                     /pseudo
     CDS             complement(6546..7694)
                     /locus_tag="PKX01_12325"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
     gene            8097..10220
                     /locus_tag="PKX01_12330"
     CDS             8097..10220
                     /locus_tag="PKX01_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ76961.1"
                     /translation="MKPLTIRRRLALGFGFVIALGLAVAVNAIYNLYALQQQFGAYEA
                     QMVARERLALRGQAMLGNGIHYFKNTVLRGGDYPAKFAQSMDALERIADEYRALGPVG
                     AEAEAALGEIRAGSVRYRSAVARAMELRAGGASIGDVDKAIAGADKPIGAALATLIDT
                     QSADTRAAREAFDAAVGRTARMIGLLAAGMVVAGALAAWRISRSVVRPLSQAVAISQA
                     VAAGTLPEHIDIATRDETGQLLEALQQMVGTLRGFVDAQRENAHQHDLGMIDQQIDAA
                     RFPGVYGDMAHSINALVQAHIAVKMQVVEVVKRYAVGDLSVDMARLPGQKAQITAAMD
                     SVKGNLQRVNAQIQTLVDAAASGDFTARGDAEAFDHAFRAMILSLNRLMDVADTGLGD
                     VSRLLGALAQGDLTQRIDGQYQGTFARLRDDANATVTQLQKLVAGLQQSAQAIATAAG
                     EIAAGNADLSARTEQQAGSLEETASSMEQLSATVRQNADSARRAHDLTRQSCHVVDQG
                     GSTVKRVVATMADIRESSGKIADIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAV
                     VANEVRQLAQRSAVSAREIKELIAVSVGKVEDGSVLADEAGHVMEEVVEVFGQVTTLV
                     TDISNASREQSVGIEQVSTAVGRMDEVTQQNAALVEEAAAAAQSLDEQAANLVAAVGR
                     FRLPARPASAAPLQPTGASRRVRSA"
     gene            10348..11064
                     /locus_tag="PKX01_12335"
     CDS             10348..11064
                     /locus_tag="PKX01_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76962.1"
                     /translation="MSARRFSPPRAILVALPLSLMLLACGGGGGGTSVPVASTASFNL
                     QAVQAAEFTTAAARPFTLSGTASGYAVTGSGTEVAGSVTAGTFEGLPALQRTTTVTGS
                     VTVNGVTTPLTTSTVDWSDTHYVPVGAVGGEYVVVQGTPTLPSAAHVGDAGLVATANR
                     YADSSKTSLQGTVRTTYGLAADTATTAILSLTQTYYDTSSAQVEVVITQYRIDTSNGF
                     TPLRASDTDYTRNVSLLMTY"
     gene            complement(11103..12386)
                     /locus_tag="PKX01_12340"
     CDS             complement(11103..12386)
                     /locus_tag="PKX01_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HMZ76963.1"
                     /translation="MMDALLFGLFALLMFAGVPLAVAMGLAGVAVVAAADLGMLSLPT
                     NVYTGIAKYPLMAIPVFVIAGLIFERAGVAATIVRFASALVGQRRGSLAIVAILVAMI
                     LGGISGSGPADSAAVGAVMLPSMLKAGYPRAFTAGVIAAAGSTDILIPPSIAFVIYSL
                     LVPQASVPALFAAGMIPGILSGLTLVLVAWWLSVRHGFEANVADERPPFWQSLKDAGW
                     GLLAPVIILGGMRSGLFTPTEAAVVAVFYGVVVGVLIYRSLGWRDIVEVLVESAEISS
                     VILTVVALASVFAWASNTLGTFDHLAAALLGTGLSEIPMLLSIQLLLLIAGMFLDAIS
                     IYFIFLPLLVPIAQHFGWDLVWFGVIITMNMALGQFHPPLGVNLMVTCRMAGVSMEST
                     VPWVLWMIAAMAGTMFLVTFIPDLALWLPRHLGYL"
     gene            complement(12383..12868)
                     /locus_tag="PKX01_12345"
     CDS             complement(12383..12868)
                     /locus_tag="PKX01_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HMZ76964.1"
                     /translation="MSEPDSSTPAGHSPLARVERFLMAASMGVLCLLTMANVLVRYFT
                     DFSFAFTEEISVSLLVIMTLVGASHAFATRHHIAITFFVDRWSALRPAAERLAVLCGL
                     AMFGLLAWYGTRMAWDDYSFEVTSPSLGVPQWWYTVWLPVLSVLIVVRLALLCFRRTP
                     R"
     gene            complement(12935..13954)
                     /locus_tag="PKX01_12350"
     CDS             complement(12935..13954)
                     /locus_tag="PKX01_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HMZ76965.1"
                     /translation="MLRRSFVLATAALLTAGLAQAQAPGYRAEYRLSTTLGTAFPWGQ
                     AGERWIELVKEKTQGRINIKLYSGNSLVGGDQTREFTAIRQGVIDLSIGSTINWSPQV
                     KELNLFSLPFLMPDYKAMDALTHGEVGKALFEVLEKRDVIPLAWGENGFRELSNSKRP
                     VASPADLKGLKLRVVGSPLYNETFTALGANPTQMSWADAQPALASGAVDGQENPLSVF
                     VAAKLPTVGQKHLTLWHYVADPLIFVVNKNVWNSWTPADREAVRQAAIQAGRENVDKA
                     RKGIAGNDTAVLRQIEASGVTVTQLSPEQRSAFVQATRPVYDKWSRTIGADLVKQAEA
                     AIARR"
     gene            complement(14126..14743)
                     /locus_tag="PKX01_12355"
     CDS             complement(14126..14743)
                     /locus_tag="PKX01_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HMZ76966.1"
                     /translation="MCANFRPSSREALSAFPLAPPDFAYGEAYPGSIVPVVTTFAPRL
                     WVPATFGLVPAWAKDARIQRSTYNARSETVGEKPSFRSAWTRGQLCIIPAAAIFEPCY
                     ESGRAVRWRVERIDGKPMGIAGIWERRLHGDGRPSWSMAMLTINADDEPLFQRFHKPG
                     DEKRAVVILPDEAWGDWLRCKSAVAAQALLQPFDAAALVACPDPR"
BASE COUNT         2426 a         5383 c         4860 g         2112 t
ORIGIN      
        1 cgcacttcct tggccaccga gaaggtgcgc tggaagagct tgtgcatggt ggcgccgagg
       61 gtgccggcct cctcggcctg gcgcacggcg tccttcatct ggccgaggat ctggggctcg
      121 cccagcacca tggaatccag cccgctggcc acgcggaaga cgtggcgcac cgcgtcgcgc
      181 tgggggtagg tgtagaggta gggggagagt tcctgcagcg ccacccggtg gtagtcggcg
      241 aaccagtcgg cggcggactg gggttcgcca gtggcgaagt acatctcggt gcggttgcag
      301 gtggacagga tcgccgcttc cttcaccggc ttgcgctgga cgaaatcgcc gagggcttcc
      361 accaggcgct ccggacgaaa cgcgaccttc tcgcggatgg cgagaggcgc ggtatggtgg
      421 ttcagtccga gagcaaacag ttgcatgcgc aacgcccggc gacgggcagg attttccagc
      481 gggcgattat atcacccgcg cttttcagcg ctgccagccc cctccacggc accggacgtc
      541 cgcgccagct cggcccgcaa ccagtcccgg aaggcccgca cgggacggcg ctcggcctgc
      601 gcttcggcgg tcaccatgta ataggcgtag ggcgaggcga ggcgctcggg aaagggctgg
      661 cgcaggcgcc cggcagcgat gtcggccgcc accaggggcg ccagggtcag caccacgccc
      721 tgcccggcga gggccgcctc caccgccagc acggcactgg aaaagcgcgg cccgcggctc
      781 acgtcggcgt ctgccacgcc gacccggcgg aaccactcgg cccagctcgg gcgcacactg
      841 gcgtccggga tggactcgtc gtggatcagc ggatgggcca ggagatcggc gggctgacgc
      901 agggccgaag cggccaccag atcggggcgc cccaccacga tgtagtccgg cgtcaggacg
      961 cgctccaccc ggaagccggg gtagcggccg ttgccgaagc ggatcgccag cacgtccggc
     1021 tctgccgcca gatcggcgag gcggtcttcg ggcggcgcgc cgacaccgtc gatattggcc
     1081 ccatcgctcg ccacacgcag ggcgatttcc ggatgggccg cagcaaagcc tgccagccgc
     1141 ggcaccagcc agcgctgggc gaaagagggt ggcgcgtgca ccgtgagccc cccttcgccg
     1201 gcctcccgcg ccgcctcaat ggccgcggcg aaacattcaa agccctcctg cacccgcggc
     1261 agcagcgcgc gtccctgggc cgtgagcacc accgcgcggg gcaggcgctc gaacagggcc
     1321 acgccgaggt aggactccag ggccttgatc tgatggctca ccgctgccgg cgtgacgtgc
     1381 agctcccgtg ccgccagggc aaaactcaga tgtcgcgcgg cggcttcgaa cgccctcagg
     1441 gacgagaggg gaggcagacg gtaggccatg actgcacacc ttagaaaaac taagcacttc
     1501 catcataaag agtcgtttgt tgcagcgcaa catcgcccct atagtgaagt catggaaacc
     1561 ccgacggaga gtccggaacc cggtcgcaga accgggcgta ccggatcggg cgctcccctg
     1621 tttcaacttt tcaggagtga ccatcatgaa actcgattcc ctttacgacc acctgacggc
     1681 cgaactggat caccgcgtcg aagtggaacg ccacagcctg agcatcgccg cccacgaagc
     1741 cggcgagtac gaagcctact tcaaggaagt cttcggcggc tcggtattct gacgctccgg
     1801 ctacggcccc ctccgtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     1921 cgcggcggag gcctgccggc acgccccgcg gctccgctcc cccttgctcc gctcttcccg
     1981 tcgccccgcc gcatcgctat agtcgggccc cgcggtctgt gtaccgcatg caagcctgac
     2041 tcccggcgcg cgcccctggg gcgttcaccg gacccatcat gccggggccg acctccccgt
     2101 cccggcggcg ggtgctgcgg cgacgcacac ccttgaggag cgaaccatga tttccgtgcc
     2161 cttcccacgg cgcgtgctgc tgcgcgccgc tgcggcgacg ctgctggccg ccgcagccgg
     2221 cctgccctgc gccgccttcg ccgaggacgt cctgcgcgtc tcggccattc ccgacgaagc
     2281 ccccaccgaa ctgcaacgca agttcgcccc cctcggcgcc tacctggaac aggaaaccgg
     2341 catgaaggtg agcttcgtgc ccgtcaccga ctacgccgcc gtggtcgagg ccctggccac
     2401 ccgcaagatc gacatggcgt ggctgggcgg tttcaccttc gtacaggcca aactgcgcac
     2461 ccacggcacc gccgtgcccc tggtgcagcg ggccgaggac gccaaattca cgtccaagtt
     2521 catcaccgcc caggacggca tcaagagcct ggccgacctg aagggcaaga ccttcgcctt
     2581 cggcgcgccg tcctccacct ccggccacct gatgccgcgc tattttctcg gccaggaggg
     2641 cctcaacccg gacaaggatt tcaagaccgt cgccttctcc ggcgcccacg acgccaccgt
     2701 ggccttcgtc caggcaggca aggtggacgc gggcgtactc aacgcctccg tgtgggacaa
     2761 actgcttgaa cagaagaagg tggacaccgc caaggtgcgc gtcttcgcca ccacgccacc
     2821 cttcttcgac tacaactgga cggtgcgcgg cgacctggat cccaagctcc aggcccgcat
     2881 cacccaggcc ttcctcaagc tcgacccggc caaccccgcc cacaaggaga tcatggccct
     2941 gcagcgcgcc agcaagttca tccccaccaa ggccgaaaac tacgccggca tcgaaaccgc
     3001 ggcccacgcc gcgggcctgc tcaagtaagc cccggccgga cgccggcagc gcgccgccgc
     3061 ccgaccgccg aaagaccgcc atgggcttct ccctcaaagg cgtgggcctg gtccacgcca
     3121 acggccaccg tgcgctgcag ggcatcgacc tgtccatcgc cgacggcgag cgggtggccc
     3181 tcgtcggcgc atccggcgcc ggcaagacca ccctgctgcg cctgctggcc gccgcccggc
     3241 gcccgacgga agggtgcctg cacctgctgg gcgccgaccc ctggagcgcc tcgccggccg
     3301 ccctgcgccg cctgcgggca cgcatcgggc tggtgcacca gtcgcccccc atcccgccgc
     3361 gccagcgcgt ggtgaccgcc gtgctggccg gccgtctcgg ccactggccc ctgtggaagg
     3421 ccctcgcctc cctcgtcctg cccgccgatg cccccggcgc cgcccgcgag ctcgcccgcc
     3481 tcgatctgac cgacaaactc tacgaacgct gcgaccgcct ttccggcggc cagctccagc
     3541 gcgtcgccct ggcgcgggtg ctgtaccagc aacccgacct cttcctcgcc gacgagccgg
     3601 tctccgccct cgatccggcc ctgtcggaac tcaccgtcgc cctgctcaac gaagaagcga
     3661 cccgccgcgg cgtcaccctg gtggccagcc tgcacgcggt ggatctggcc ctgcgccact
     3721 tcccgcgggt ggtcggcctg caggacggcc gcatcctctt tgacctgccc agccgcgagg
     3781 tgagcgccgc ccagctgcgc accctgtacg cggccgaagg cgggctgccc gtcgccagca
     3841 tttgtccggc cgggcagcct tcgaggcgct gctcgtgaat cgccccggca aggacccacg
     3901 ctacgtgccg tgccccgatc agcccggacg ccaacccacg cgcgccggcc caccggcgct
     3961 ccacccgcgc accgcccgtc tcgcctcaac cccatgagcc ccaccccgcg cgacccggcc
     4021 gccctccccc gtctgggcgc caccgttctg gccctggcgc tgctggggcc actgctgcac
     4081 ctgacggact tccgccccgc cgccctgttc gaagccggca acctgcgcac ggtgggccag
     4141 tttctcggcg gcttcctgcc gccggcgcgc gatccggact tcctcgccct cctcgcccgc
     4201 gccaccgtgg aaacccttgc gatggccacc gccggcatgg ccgtggcctt tctgctcgcc
     4261 ttaccgctgg gcctcgccat ggcccgcagc ctgtccgtgt cgcgtctggc cgacgcgcgg
     4321 ggcagccgca ccggcgccct cctgcgcgcc ctcgtccgtg gcctgctgac ccttctgcga
     4381 ggcattccgg aactggtctg ggcgctgctg ttcgtacggg tcttcgggct cggtccggcg
     4441 gccggcgtgc tggccctggg catcacctac ggcggcatgc tcggcaaggt ctacggcgag
     4501 atcctcgaat ccacagacgc ccgcccggcc cgcggcctgc tggaggccgg cagcggacgc
     4561 ctggcggcct tcagctacgg cttgctgccc ctcgccgccc cggagctggt ctcctacacc
     4621 gtgtatcgct gggaatgcgc ggtacgtgcc tcggtggtga tgggcttcgt cggcgccggc
     4681 ggactcggcc agttgatgga ccagtccatg aagatgctca acggcggcga ggcggccacc
     4741 atcctcgcca gcttcctgct cctcgtggtg gctgccgacg ccctctcgga gtggctgcgg
     4801 agccggctgg catgaacgta cgcacccctt cttccccacg cgtggccggc gtcacacccg
     4861 gcggcctgct cgccggtcta gcggtgctgg ccgcggtggc ggggagcttc gtcttcctcg
     4921 acctgcccct gacggccgtg ttcaccggcg cggcggcaac agaggcgctg gacttcgcgg
     4981 gacgcttcct gccaccggag acggacaccg acttcatggc caaggtcggt ggcgcggcgc
     5041 tggaaaccct ggccatttcc ctcgtcgcca ccctgctggc agcggcgggc ggcgccctgc
     5101 tggccctccc cgcctcgggc cgccatggcc gtccggcgcg gatcgtggcg cgcttcgtgc
     5161 tcaatctgct gcgctcggtg cccgaactgg tgtgggcgac cctgctggtc ctggcagccg
     5221 ggctcggccc cttcgccggc accctggcgc tggccgtgca caccaccgga gtgctcggcc
     5281 gactctttgc cgaggccctc gaaaacgcgc cggccgcgcc cgccgaagcc ctcaccggcg
     5341 gcggcgcgcc cgccctcgcc agcttcctct acggcaccct gccgcaggtg ggcgaccaat
     5401 ggctcgccta cgccctgtac cgctgggaga tgaacatccg catggctgcc atcctcggtt
     5461 tcgtaggcgc cggcgggctg ggcggcctgc tgtactacag cctgtccctg ttccgcgagg
     5521 ccgaagccgg cacggtgatc ctggccatgc tggtcatggc gatcgccgtg gacagcgtca
     5581 gcgcctggct gcgacgggcg cgctgaaagt ctccgccccg cagccctgca taagccgcag
     5641 cgagccggcg caatcgcgcc ggatcaatgc tggttcagct gggcttccgt gagctgccgg
     5701 aggcggtcgg ccacattgcg ggtgtggtcc gcgttgcccg cggtgcgact gctgatcccc
     5761 gcgatatccg tgagcaggga cgacaggcgc gtgacatcgc cggcctgctg ctcgatctgc
     5821 agggcgacgc gaccgatctc gccggcaagg gcggagatgt cggcgccgat ctcgccggcg
     5881 ctggtgcggg acttctccgc cagcttgcgc acttcgtcgg ccacgacggc aaacccgcgg
     5941 ccggcctcgc ctgcccgcgc ggcctcgatg gctgcgttga gggccagcag attggtctgg
     6001 tcggcgatct gctggatcac ctccaccacg ctctggatgc ggttgctggc gcgggccagg
     6061 tcgcgggcgc tgccggccgc atcggcagcc tggcggctca ggtcggacac ccgcccgccg
     6121 gcatcgtgca ccacccgctc ctgttcgtgc acgtcacctc cgagggcatc catcgatgcg
     6181 tagaggtcgc ccgcgcaggc ctcgagctgg cgcgccgtgt cgtcccgcag gacatgggac
     6241 gccttgaggg tctcaccgag ggtgttgagc ccatgggcca ccagcgagat gttgtcgtcg
     6301 aagcccgacg ggtcgagctg gctgtcgaac tgcccggcct ggtaggacaa gatctgctgg
     6361 acgatgacgc gcgagaggcc ggtgagcttg ttgctctggc ggcgcaggaa gaaggccttg
     6421 aactccccgt aatgggccac gaagttgtcc acgaactcgt cacggaagcc ggcgccctcc
     6481 gccgcccgga aaaagaagat cgccgtcagg gtctggttga ggttgaggcc caggatttcg
     6541 ccgaaggtgg agaaacctgc cagcgacgga ctggccagca ccgatccgag cttgcccaac
     6601 tccggcccgt tgtagaggcg ccgcaggatg cagtcgttga ggatgccggc caccggctgg
     6661 cctggcttgc cctccatgaa ggtccggaag tcccgctcgg tgctgtgcac caggctggtc
     6721 cgacgcacca gcagcaggtc ttcacccgcc gcgatgtcac agtagaagcc cacccgcccg
     6781 tgggccaggt cgatgcccga gacggagcgc acgaatagtt cgtcgcccac ccggatggcg
     6841 aaggaatagt ccgcgaggcg cgcgtccagt tcgtgaggcg tacagcgcag ctcctcgcac
     6901 agggcgtcga tgaaggacac gatgcgcccc tccttgtcga tcacctggcg cacgaagcgc
     6961 tgttccagcg acgcgccaat gaccgggaag tgcgcccccg cctcctcgaa gttgtggctc
     7021 ttgaacaccc cgaagcgcat cccccggggc agcttgagga aggcgatgag ggcatggttc
     7081 tccagcttgc gatggccgtc gtgcagccag gtgtggcgga agtccagctt gccgcccgcc
     7141 gaccctccca cgaacagaca ggggaaacgc ccggaatcgt agagggcctc catgaagaag
     7201 gactcggacg cggacagtcc gtcgaacagg atataggcca gcgtgtcccg atggtcgata
     7261 tccatctcga ccttcaggct gcggatctcc tcgcgcaggg tggccacccg ctcacggacg
     7321 tcctgatcgc accgaccgcc ccgcaggtct tcacacctga gcggcacggc caccacttcg
     7381 gcacgggcga tcagggtcgc gtcgaagagc tgcaccacca cccggtccca ggcatcgccg
     7441 gtcgggcaat acaggtcacg ccccgtggcg cacagttcgc ccgccgtgga gcacagcatc
     7501 accggtacgc cgggaaaccg ggcggtcacc gcccgtgcca ccggttccag gtccacatgg
     7561 ggcgacacga agccacagac gaaggccggc ggcgccgtca ccgcggcgag gcgtgccgac
     7621 agttcggcac tgcggcactg gagcgtctga acctcggcca ccggcccgcc cagaaacctg
     7681 tcgaacagct tcatgtcttg tctcctcttg ttctcatcgt gggcccattc ggcgcgttac
     7741 gcctcctatc ggcggccatc cggcgacctt gaatgcccga agcaggcgcg cacaagacca
     7801 ccccacgcat gcggcatcgg agcatgcccg gcgacttgcc cggacctgcg ccaggaccac
     7861 gcaggcatca ggatctgcgg ccatcggacc gtcacacgcc tgcccgccgc gacgaccgga
     7921 ttcatgtttg cctccagacg gtcgttattc gctccacgcc ccgcgcccct tggacgcccg
     7981 tcacccgccc ccagaccgac catgcccctg caccctctgg ctgaccgcag caccggtcat
     8041 cgcatcgacg tcgcccccct tgccccagcc gtcagcccga cacggagaat cacgccatga
     8101 agccgctcac catccgccgt cgcctggccc tcggcttcgg cttcgtgatc gccctcggcc
     8161 tcgccgtggc ggtcaacgcc atctacaacc tgtacgcgct gcagcagcag ttcggtgcgt
     8221 acgaagcaca gatggtggcg cgggaacgcc tggccctgcg cgggcaggcc atgctcggca
     8281 atggcatcca ctatttcaag aacacggtct tgcggggcgg cgactacccg gccaaattcg
     8341 cccagagcat ggacgcgctg gaacgcatcg ccgacgagta ccgcgccctt ggcccggtcg
     8401 gcgccgaggc cgaggcggcg ctcggcgaga tccgggccgg cagcgtccgt taccgcagcg
     8461 ctgtggcgcg ggccatggag ctgcgcgccg gcggggcgtc catcggcgac gtggacaagg
     8521 ccatcgccgg ggccgacaaa ccgattggcg ccgcattggc gaccctgatc gacacccagt
     8581 cggcagatac ccgcgcagcc cgcgaggcct tcgacgcggc cgtcggccgc accgcccgga
     8641 tgattggact gctcgccgcc gggatggtcg tcgcgggggc gctggccgcc tggcggatca
     8701 gccgcagcgt cgtccgcccc ctgagccagg ctgtcgccat cagtcaggca gtcgccgccg
     8761 gcaccctgcc cgagcacatc gacattgcga cgcgggacga gacaggccag ctcctggaag
     8821 ccctgcagca gatggttggc acccttcgcg gctttgtgga cgcccagcgg gagaacgcgc
     8881 accagcacga tctgggcatg atcgaccagc agatcgacgc ggcccgcttc ccgggcgtct
     8941 acggcgacat ggcgcactcc atcaacgcac tggtgcaggc ccatatcgcc gtgaagatgc
     9001 aggtggtgga ggtggtcaaa cgctacgcgg tgggcgacct gtcggtggac atggcgcgcc
     9061 tgcccggtca gaaggcccag atcaccgcgg ccatggacag cgtcaagggc aacctgcagc
     9121 gggtaaacgc gcagatccag accctggtgg atgccgccgc gagcggcgat ttcaccgccc
     9181 gcggcgacgc cgaagccttt gaccacgcct tccgcgccat gatcctcagc ctcaaccgac
     9241 tgatggatgt ggccgacacc ggactgggcg acgtctcgcg cctgctgggc gcactcgccc
     9301 agggcgacct gacccagcgc atcgacggcc agtaccaggg caccttcgcg cggctgcggg
     9361 acgatgccaa cgccaccgtc acccagttgc agaagctggt ggcgggcctg cagcagagcg
     9421 cccaggccat cgccaccgcc gcgggcgaga tcgccgccgg caacgcggac ctgtctgccc
     9481 gcaccgagca gcaggcgggc agtctggaag agacggccag ttcaatggag caactcagcg
     9541 ccaccgtgcg gcagaacgcg gacagtgcga gacgtgccca tgacctcacg cgccagtcct
     9601 gtcacgtggt cgaccagggc ggcagcactg tcaagcgggt ggtcgccacc atggcggaca
     9661 tccgcgaaag ctcgggaaag atcgccgaca tcatcggcgt catcgacagc atcgccttcc
     9721 agaccaacat cctggccctg aacgcagccg tcgaggcggc ccgcgcaggg gaacagggtc
     9781 gcggctttgc cgtggtcgcc aacgaggtcc gccagcttgc ccagcgcagc gcggtctcgg
     9841 cccgcgagat caaggaactg attgccgtct cggtgggcaa ggtggaagac ggctcggtcc
     9901 tggccgacga ggccgggcat gtcatggaag aagtggtcga ggtcttcggc caggtcacca
     9961 cgctggtcac cgacatcagc aatgccagcc gcgagcagag tgtcggcatc gagcaggtca
    10021 gcaccgccgt cggccgcatg gacgaagtca cccagcagaa cgccgcgctc gtcgaggaag
    10081 ctgccgctgc cgcacagagt ctggatgagc aggcggcgaa tctggtggcc gccgtcggcc
    10141 gcttccgcct gcccgcgcgt ccggccagcg ccgcgccgct tcaacccacc ggcgcgtcgc
    10201 gccgcgtacg cagcgcctga aagccacatc cggagcgccc ggcagccgac acgggcccgc
    10261 aagaacatcg tcatccacgg cccctgtccg gccctgtcgg gcgtaatatc cgctctcgct
    10321 gatattcccg tctgtccgga gcctgccatg tctgcccgcc gtttctcccc cccgcgtgcc
    10381 atcctcgtcg cgctgcccct ctccctgatg ctgctcgcct gcggtggcgg cggcggaggc
    10441 acgtccgtac cggtcgcttc caccgccagc ttcaacctgc aggccgtgca ggccgccgag
    10501 ttcactaccg cagccgcccg cccattcacc ctcagcggca ccgccagcgg ctacgccgtc
    10561 acgggcagcg gcaccgaggt cgccggcagc gtcacggccg gcacctttga aggcctcccg
    10621 gccctgcagc gcaccaccac ggtgaccggg tccgtgacgg tcaatggtgt caccaccccc
    10681 ctcaccacct cgacggtgga ctggagcgac acccactacg tgcccgtcgg cgccgtcggc
    10741 ggcgagtacg tggtggtgca gggcacgccg accctgccca gcgccgccca cgtgggcgac
    10801 gcgggcctcg tcgccaccgc caatcgttac gccgatagct ccaagaccag cctgcagggc
    10861 acggtgcgga ccacctacgg actggccgcc gacaccgcca ccacggccat cctgtccctg
    10921 acccagacct attacgacac gtccagcgcg caggtcgagg tggtgatcac ccagtaccgc
    10981 atcgacacgt caaacggttt cacgccgctg cgggcgtcgg acaccgacta cacccgcaac
    11041 gtgtccctgc tgatgaccta ctgagaatca cccggtgggg gccccacgcc cccgcggtcg
    11101 gctcacagat agcccaggtg gcgcggcagc cacagggcca ggtcggggat gaaggtgacc
    11161 aggaacatgg tgcccgccat cgccgcgatc atccacagca cccagggcac cgtggactcc
    11221 atggacacgc cggccatgcg gcaggtgacc atcaggttca cacccagggg cgggtggaac
    11281 tgacccagag ccatgttcat cgtgatgatg acgccgaacc acaccaggtc ccagccgaag
    11341 tgctgggcga tgggcaccag cagcggcagg aagatgaagt agatggagat ggcgtccagg
    11401 aacatgccgg cgatcagcag caggagctga atcgacagca gcatggggat ctccgacagc
    11461 ccggtaccca gcagcgccgc cgccaggtgg tcgaaggtgc cgagggtgtt gctggcccac
    11521 gcaaagacgc tggccagggc caccacggtg aggatcaccg acgagatctc ggcggactcc
    11581 accagcacct cgacgatgtc ccgccagccg aggctgcggt agatcagcac accgaccacg
    11641 acgccgtaga acaccgccac caccgccgcc tcggtgggcg tgaacaggcc gctgcgcatg
    11701 ccgccgagga tgatcaccgg tgccagcagg ccccagccgg cgtccttgag gctctgccag
    11761 aagggcgggc gctcgtccgc cacgttcgcc tcgaacccat gccgcaccga cagccaccag
    11821 gcgaccagca ccagggtgag gcccgacagg atgccgggga tcatgccggc ggcaaagagc
    11881 gccggcaccg aggcctgggg caccagcagg ctgtagatga cgaaggcgat ggagggcggg
    11941 atcaggatgt ccgtcgagcc cgccgcggcg atcaccccgg cggtgaaggc gcgcggatag
    12001 ccggccttga gcatggacgg cagcatcacc gcgcccaccg cggcggaatc cgccggaccg
    12061 gagcccgaga tgccgcccag gatcatcgcc accaggatcg ccacgatggc caggctgccg
    12121 cgccgctgcc ccaccagggc cgatgcgaag cgcacgatgg tggccgccac gccggcccgc
    12181 tcgaagatga gcccggcgat gacgaagacc gggatcgcca tcagcgggta cttggcgata
    12241 cccgtgtaga cgttggtggg cagggacagc atgcccaggt ccgccgccgc caccactgcc
    12301 acgccggcca ggcccatcgc cacggccagg ggcacgcccg cgaacatcag cagggcgaag
    12361 agcccgaaga gcagcgcgtc catcatcgcg gcgtcctccg gaagcacagc agggccagac
    12421 gcacgacgat cagcaccgac agcaccggca gccagaccgt gtaccaccac tggggcacac
    12481 cgagggacgg cgacgtcacc tcgaagctgt agtcatccca agccatccgc gtgccatacc
    12541 aggccagcag gccgaacatg gccagcccgc acagcacggc cagccgctcc gccgcaggac
    12601 gcagcgccga ccagcgatcc acgaagaagg tgatggcgat gtggtgccgc gtggcaaagg
    12661 cgtgggacgc ccccaccagg gtcatgatca ccagcaggga gacggaaatc tcctcggtga
    12721 aggcgaagga gaagtcggtg aaatagcgca ccagcacgtt ggccatggtg agcaggcaca
    12781 gcacgcccat ggaggcggcc atcaggaagc gttcgacccg cgccagcggc gaatggccgg
    12841 cgggggtgga ggaatcgggt tcggacatgg ggaaaatgcc gtgaagcacc ggagcggtcg
    12901 gcccgctccg gcattgaagg gggacggacg gcgctcagcg cctggcgatg gcggcttcgg
    12961 cctgcttgac gaggtctgcg ccgatggtcc gggaccactt gtcatagacc gggcgggtgg
    13021 cctgcacgaa ggcgctgcgc tgctctggcg agagctgcgt gacggtgacg ccggacgcct
    13081 cgatctgcct gagcaccgcg gtgtcattgc cggcgatgcc cttgcgcgcc ttgtccacgt
    13141 tctcacggcc ggcctggatc gccgcctggc gcaccgcttc ccggtcggcc ggggtccagc
    13201 tgttccagac gttcttgttc accacgaaga tcagcggatc tgcgacgtag tgccacaggg
    13261 tcaggtgctt ctgaccgacc gtgggcaact tggcggcaac gaacaccgac aggggattct
    13321 cctggccgtc caccgcgccg gacgccagcg ccggctgggc gtcggcccag ctcatctggg
    13381 tggggttggc gcccagggcg gtgaaggtct cgttgtatag gggcgagccg acgacacgca
    13441 gcttgagccc cttcaggtct gccggcgagg ccaccggccg cttggagttg gatagctcac
    13501 ggaaaccgtt ttcgccccag gccaggggga tcacgtcacg tttttcgagc acctcaaaga
    13561 gggccttgcc cacctcgccg tgggtaaggg cgtccatcgc cttgtagtcc ggcatcagga
    13621 agggcagcga gaacaggttg agctccttga cctggggcga ccagttgatc gtggagccga
    13681 tggacaggtc gatcacgccc tggcggatcg ccgtgaactc ccgcgtctgg tccccgccca
    13741 ccagggaatt gccggaatac agcttgatgt tgatccggcc ctgggtcttc tccttgacca
    13801 gctcgatcca gcgctccccc gcctgccccc acgggaaggc cgtgccgagg gtggtggaca
    13861 ggcgatactc cgcccgatac cccggcgcct gggcctgggc cagaccggcg gtcagaaggg
    13921 cggcagtcgc caggacaaag gatcggcgca gcatggggaa ctcctcggaa gggaaagagg
    13981 acgattatcg gcgtcgcaca tgcgggcggg caagcgcggg ctgccgccgc cgtgcgggga
    14041 tctgcgcccg gagctcggcg gaggcggatg tcctgccacc gcgccccacc cgcaacgtgg
    14101 gggccagaca cagagaatgc cggcgtcaac gtgggtcagg gcacgcgacg agcgccgctg
    14161 catcaaacgg ctgcagcaag gcctgggctg cgacggcaga cttgcaacgc agccagtccc
    14221 cccacgcctc gtcgggcagg atcaccaccg cccgcttttc gtcgcccggc ttgtggaagc
    14281 gttggaacag cggctcgtca tccgcgttga tggtcagcat ggccatggac caggacggtc
    14341 gtccgtcgcc atgcaggcgt cgctcccaga tcccggcgat ccccatcggc ttgccgtcga
    14401 tccgctcgac gcgccagcgc accgccctgc cggactcgta acagggctca aagatcgcgg
    14461 cggccggaat gatgcagagc tggccgcgcg tccatgcact gcgaaacgag ggcttctcgc
    14521 ccacggtctc cgagcgcgcg ttatacgtgg accgctggat ccgcgcatcc ttcgcccacg
    14581 caggcaccag accgaaggtc gccggcaccc acaggcgcgg cgcaaaggtg gtgacgaccg
    14641 gtacgatgct gccagggtag gcttcgccat aggcgaaatc cggcggcgcc aaggggaagg
    14701 cactcagcgc ttcgcgggaa gacggtcgaa aattggcgca catggttcaa gcgtagcgcg
    14761 tctgtcggcc catcaccggc ttccacaagg cacctggcga cgcattgggg aacgaaccgg
    14821 cccagacaaa caaaaagccc aaccggtcac ggctgggctt tttgtctgaa atatctggtg
    14881 g
//
LOCUS       DAOQPA010000090        14811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2063_length_14811_cov_17.507048, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000090 DAOQPA010000000
VERSION     DAOQPA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_14811_cov_17.507048"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1266)
                     /gene="hemB"
                     /locus_tag="PKX01_12360"
     CDS             complement(259..1266)
                     /gene="hemB"
                     /locus_tag="PKX01_12360"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMZ76967.1"
                     /translation="MSPVSAVFPGTRMRRMRRDDFSRRLMREHRLSADDLIYPVFVLE
                     GQDVVQPVASMPGVSRVSLDRLLMIAEEAVALGVPALALFPVVDADKKSLGAEEAFNP
                     DGLVPRVVQALKQRFPELGVITDVALDPYTSHGQDGLIDETGYVLNDETLEVLAKQAL
                     CHARAGADVVAPSDMMDGRVARFFFNDTATTEIYTRILAYSAKYASSFYGPFRDAVGS
                     AANLGKGNKYTYQMDPANSDEAIREVALDISEGADMFMVKPGMPYLDIVRRVKTELQV
                     PTYVYQVSGEYAMLKAASANGWLNDRACALEALLAFKRAGADGILTYYALEAARWLKE
                     G"
     gene            complement(1599..1874)
                     /locus_tag="PKX01_12365"
     CDS             complement(1599..1874)
                     /locus_tag="PKX01_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ76968.1"
                     /translation="MNKTELIDALAGKTGMTKVEAGRAVDGVVEIVTEALAKGESVTL
                     VGFGTFAVGERAARVGRNPQTGAELNIPASKQPKFSAGSKLKDAVNK"
     gene            complement(2070..3446)
                     /gene="radA"
                     /locus_tag="PKX01_12370"
     CDS             complement(2070..3446)
                     /gene="radA"
                     /locus_tag="PKX01_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMZ76969.1"
                     /translation="MAKPRSIYVCRECGGRAPRWQGQCPHCQAWNTLDESVVEPEAPA
                     NRRFAALAADTGRLQSLAEVQPRESPRVPTGIEEFDRVLGGGLVPGGVVLIGGDPGIG
                     KSTLLLQALSLLAGRLKALYVSGEESAEQVALRAVRLHVEAHGLQLLPEINLERILAT
                     LRDVKPDVAVVDSIQTVYSEALTSAPGSVAQVRECAAQLTRFAKQGGTSLVMVGHVTK
                     DGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLKGVS
                     NPSAIFLSQHGQQVSGSCVLVTQEGTRPLLVEIQALVDSAHSPSPRRLSVGLEQNRLA
                     MLLAVLHRHAGVVCFDQDVFVNAVGGVKIAEPAADLAVLLAIVSSLTSRPLPRELVVF
                     GEVGLAGEIRPAPRGQERLRESAKLGFSVALVPRANAPKQAIPGLRVIAVERIEEALE
                     QARGLSTG"
     gene            complement(3448..4527)
                     /gene="alr"
                     /locus_tag="PKX01_12375"
     CDS             complement(3448..4527)
                     /gene="alr"
                     /locus_tag="PKX01_12375"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HMZ76970.1"
                     /translation="MPRPIRASIDLDALRHNYRLARRHAGAAAAYAVIKANAYGHGLL
                     PVARALAPEADGFALLDLADAAQLRAAGIDAPIVLLEGFFEPADLPQLTALGVSPAIH
                     SKAQLAMLERAALDRPLDVLLKINTGMNRLGFTPERVPAALQRLQALACVRRITLMTH
                     FARADDEVGVAAQLASFHRVAEGLGLPASLANSAALLRYPDARGQIVRPGIMLYGSSP
                     MPALHSAEALGLRPVMTLASEIIAVQELRPGEQVGYGGTFEARLPTRVGVVACGYADG
                     YPRHAPTGTPILVGGRRTRTLGRVSMDMLACDLTHLPEAGIGAPVTLWGAGMPADEVA
                     EAAGTISYELFCALAPRVPVGYLGA"
     gene            complement(4532..5443)
                     /locus_tag="PKX01_12380"
     CDS             complement(4532..5443)
                     /locus_tag="PKX01_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ76971.1"
                     /translation="MADRRLQVFHAVARQGSFTRAAEALFMTQPAVTFQIKQLEEQFN
                     TRLFDRGHGRVTLTPAGELVMAYAEKILGLGEEMEARVAEMTDDLSGVLHLGTSTTIA
                     GYWLPALVAGFKQRYPRVVPRVAVGNSQLIESRVADRTLDVGLIEIVSENPTIDRRAA
                     GQDELQVILAPNHPLARATALTATDLASHPFIHREPGNAIRDLSDQFFASAGIAPEEL
                     PIAAELGSLAAVKHLTALGFGYAIASHAAIRQEIAEGSLVSVPLAPRLYTPLEVIVLK
                     DKFRSRLVNTFADFVTAEIARLSFSPA"
     gene            5622..6884
                     /gene="lplT"
                     /locus_tag="PKX01_12385"
     CDS             5622..6884
                     /gene="lplT"
                     /locus_tag="PKX01_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HMZ76972.1"
                     /translation="MPFGFYMIMAAQFFSALADNALLIASIFMLRQMQAPQEYEPLLK
                     LFFTVSYVALAAFVGAFADSMPKWRVMIISNTIKIAGCSLMFLDVHPLIAYAVVGLGA
                     AAYSPAKYGILTEYLPHRKLVVANGWIEGLTVGAIILGTLVGGMLIKPGVSQALLGID
                     VPYIDTGIDTPGEVALVVVAVLYLIAALFNLKVPDTGVDHKPLKSSPTYLVHDFIHCL
                     KLLWRDKLGQISLATTTLFWGAGATLQFIVIKWAEQALHLDLSKASMLQGVVAIGVAL
                     GAVLAAKFVSMRASVRVIPLGIAMGIIVNVMIFVTNMHVAMVLMVLIGALSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILIMTGLYALLIRADVSINTVVVLFGLFVA
                     GTMFLVKRRHEANQREFDSVAQLDDVKH"
     gene            complement(6916..7560)
                     /locus_tag="PKX01_12390"
     CDS             complement(6916..7560)
                     /locus_tag="PKX01_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ76973.1"
                     /translation="MIAGSPLRVVLADDHAIVRTGYRQLLEMEEDIVVVADFAHSDAV
                     CHWFAEHEADVLVLDLSMPGRGGLETLARVKAHRPALRVLIFSMHDSAAMVRQALRAG
                     ADGYLSKSSEPDALIQAVRTVARGGRPLSPDVAAAVSAGDGQPASLALSPREFEIFRL
                     LAAGRSVDEVAERLFLSAKTVANYQTTIRHKTGLAGPVDMYRHARAHGLLDGSE"
     gene            complement(7557..8882)
                     /locus_tag="PKX01_12395"
     CDS             complement(7557..8882)
                     /locus_tag="PKX01_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HMZ76974.1"
                     /translation="MIRLLMGRVGWTTLVCLAVFLALAAWRAEVDIRREGQGAAGTVR
                     LLEALAALQASDGASRAARLDELRQLGQAGRLRHLRFQLEDADGRVLVAAEGAPVAGG
                     RVVLKTADGQAYRVALSPDARSEQEEAAANIGGMAALFMAYGLVLLAGLYLAARHALK
                     PLRRLLEAIASLRQRDFSVRVPPLPVQELDSIGAALNHLGEGLAAAETRQRALHARLL
                     TVQDDEQARLALRVQESFGQELTALRSRVACLRRQSGNDPVLADQLAALETSCRTLDA
                     SVRGMLRDLHPDVCAGGAGDGASLAPLLHELVKSWQDAAGPGTEIHEAVMPPDRTLPG
                     AVSRTLYRMTQEALANVVRHASARCVSVSVRDVPDDAAAIEWEVRDDGCGIADLDAAM
                     GRGSGLAALRERVWALGGWLQIGPANAAEGASAVSPGLCLRARLPVLAR"
     gene            complement(8910..11408)
                     /locus_tag="PKX01_12400"
     CDS             complement(8910..11408)
                     /locus_tag="PKX01_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ76975.1"
                     /translation="MQYPAARPTQPLKPLVITLAAAWGASALPALADDATRAPTVVVV
                     GQTPLPGLDQPIFQIPAPVQFATDKDVDRVRVPDLSAFMNRSLSGVHINEVQGNPFQA
                     DVNYRGYTASPLAGTPQGLSVYLDGVRINQPFGDVVSWDMIPRSAISSVTLMPGSNPL
                     FGLNTLGGALALTTKDGRTHPGASVQVTGGSYGRRSVEFEHGGFNDKGLEWFVTGNLF
                     HEDGWRAHSPSDVHQFFGKAGWQNATTDIDLSLAVADNRLNGNGLQERRFLDQDWNSV
                     YTSPDITKNNSMFLNLTGRHSLSDDAILSGNAYYRRIAGTTFNGDVNEGALDQSVYQP
                     NAAERSALAAAGYSGYPTSGANASNTPFPSWRCIANALLNDEPNEKCTGLLNRTSTTQ
                     RNFGLSGQLSVAGTLAGRPNQFTAGAAYDQSQVNFRQSAQFGYINPDHTITPVDAYAD
                     GTQTSENAFDARVDLNGRTRTWSVFATDTLALAEKWHATLSGRYNTTTVKNRDALNPG
                     GGSASLDGDHRFSRFNPAAGITFSPTPALNAYLGYTEGSRTPTAIELGCANPDNPCKL
                     PNAMAGDPPLNQVVTRTWDAGLRGRLGAETRWNAGVFRAVNSDDILFVADNQSGYGYF
                     RNFGKTRRQGVELGASTRVGALNLSANYTWLDATYQSEELLGGSGNSSSDAASAGLDG
                     NIRVRPGDRIPLIPRQILKLSADYRVTPALAVGADLVGVSGSTARGNENGQHQADGTY
                     YLDGGRSAGYSVINLNSTYQASRGVQLFGQIGNLFDRRYTTAAQLGSTAFSSSGGVLA
                     RPFASVGGEFPLVHSTFYAPGAPRTLWVGLRYSL"
     gene            11682..13922
                     /locus_tag="PKX01_12405"
     CDS             11682..13922
                     /locus_tag="PKX01_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HMZ76976.1"
                     /translation="MPAHCPIRTPNPPASALVPLGALLFGFSLPTAAAESAPTLAPVI
                     VTDRQDVPDASNSYQGQTTTVGKLKQLPKDIPQALTIVTEKLMEDRGQSTLKDALSNV
                     AGLTFNAGEGGRIGDNMNLRGFYSFGDLYQDGIRDTAQYNRDTFNLQQIDVLRGSGSM
                     LFGRGQAGGVINQVSKTAMLYDENEVSATVGSYNTQRLTADLNRRLGDTSAVRINLMK
                     TDAGSSRDGVTSDREGIAPTLRWGIGTDDEIALSHFYLKTHNVPDYGVPFYQNRPLDV
                     PASRFYGTSRDYEDNTTNLTTGSYQHRFSADSAVKTVLRAASYDRELWAAQPQLRGSW
                     SSPNTCTAFSGTLSDASTICRSIKARGAREKTLTSQTDFTTRFTTGALKHEALTGVEF
                     LREDLSRWSHSLTGIVVPATTVGAPDPGAPGLGSAYGNQVPTSPASYTGNSRGVYAQD
                     TVEFLPGWKLLAGLRHDSLRADYLTTAAGASSPTATALGFSEMSYRSGLMFQPDDETS
                     YYLAWNDSFNPTADLYQLDVGQGFPAERSRTTEIGAKWELFDGDLSLRAALYRAVKFW
                     ERNTDLESSGGLLSKKRHTDGVELEAAGRITPKWEVFGGVALMDAVIDEAGYTLNTAT
                     GVVTAHNPNLVGMRPRNAPPYTFNLWSTYKLAGGWRVGAGVEGKGERLAYGIGTGTAA
                     ITPNVAPAYQRWDAMVAYEQPAWSVRLNVLNLFDTRYYESIYDNGGHVVPGTERAVQV
                     TTRFKF"
     gene            13943..14626
                     /locus_tag="PKX01_12410"
     CDS             13943..14626
                     /locus_tag="PKX01_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HMZ76977.1"
                     /translation="MLLHIPHVLSRDAVADCRRQLEAAAWVDGRLTAGSQSEQRKHNL
                     QLAEDSDLARALRDTVLDALGRTPTFFSAALPRRIYPPLFNCYTGAHNAFGDHVDNAV
                     RTHRATQTHLRTDLSFTLFFTDPDEYDGGELVIEDTFGCQRIKLPAGDLVLYPSSSVH
                     RVEPVTAGARIACFSWLESMVRETERRRLLYDLDRQICRLRETHGDDDTAVGLTSCYH
                     NLVRMWACP"
     gene            14623..>14811
                     /locus_tag="PKX01_12415"
     CDS             14623..>14811
                     /locus_tag="PKX01_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76978.1"
                     /translation="MSSALAMPLPHPPRPGHPARLRQSAGIAAACGLHALAGALIVHL
                     ALDTAAPQVPPQSSVAVRL"
BASE COUNT         2498 a         5316 c         4700 g         2297 t
ORIGIN      
        1 tcatccacgc ccccaacagt cgcggcaagg tgcgggtcga ccgcctcgac aaccgttact
       61 tcgccgaacg cttcgaaggg gggcggaccc tgctgccacg gggctgaggc gcacacggat
      121 gccggatggg catgggctcc ggagcgtcct gaaccgatcc catgcatgcg cgggactgcc
      181 ggacatccga agcgcggatg tccggcagtc ccgcaaggcc ccggcacacc agagcccgac
      241 caccgtgacg gagaccgttc acccttcctt cagccagcgc gccgcttcca gcgcgtaata
      301 ggtcaggatg ccatccgccc ccgcccgctt gaaagccagc agcgcctcca gtgcgcaggc
      361 ccggtcattc agccagccgt tggccgaggc tgccttcagc atcgcgtatt caccgctcac
      421 ctggtacacg taggtcggca cctgcagttc ggtcttcacc cgacggacga tgtccagata
      481 gggcatgccc ggcttcacca tgaacatgtc ggcgccttcc gaaatgtcca gggccacctc
      541 gcggatcgcc tcatccgaat tggccgggtc catctggtag gtgtatttgt tgcctttgcc
      601 cagattggcg gccgaaccca ccgcgtcgcg gaaggggccg taaaagctcg acgcgtactt
      661 ggcggaatag gccaggatgc gggtgtagat ctcggtggtc gccgtatcat taaaaaaaaa
      721 acgcgccacg cggccatcca tcatgtccga tggtgccacc acatcggcgc cggcccgggc
      781 gtggcagagg gcctgctttg ccagcacttc cagcgtctcg tcattgagca catagcccgt
      841 ctcgtcgatg aggccgtcct ggccgtggct ggtatagggg tcgagggcca cgtcggtgat
      901 cacgcccagt tccgggaagc gctgcttgag ggcctgcacc acgcggggca cgaggccatc
      961 cgggttgaag gcctcttcgg cgccgaggga tttcttgtcg gcgtccacca cgggaaacag
     1021 ggccagggcc ggtacgccca gcgccaccgc ctcttcggca atcatcaaca gacggtccag
     1081 ggatacgcgc gaaacaccgg gcatggaggc aaccggctgc actacatcct gcccctccag
     1141 cacgaagacc ggatagatca gatcatctgc agacaggcga tgctcgcgca tcaggcgacg
     1201 ggaaaaatcg tcccggcgca tgcgacgcat gcgggtgccg ggaaagacgg cggaaactgg
     1261 gctcatggaa ttctcgctgg cgtacagact tacatctcga aacgaagggc cccgggaact
     1321 tttaccccgc tgaccctgac caaggtacag atggtgctga accgtctccc gctatacctc
     1381 cctgagcggg gccttgattg catgcaaggc gttttagccc ccggtcaccc ccctggccgg
     1441 gggttttctt tttcggggcg cccaatccgg ctcggccgga aacccgccag gcgccgagga
     1501 tacgcccccg ccgcccataa aaaaacaggt gggacccgcg aaaggcacca cctgacatga
     1561 ctgagtccgg tacgccatac gcgcaccgag tcgtcgcatt acttgttcac ggcatccttc
     1621 agcttggaac cggccgagaa cttgggctgc ttggacgccg ggatgttcag ctcggcaccg
     1681 gtctggggat tgcggcccac gcgggcggcg cgctcgccga cggcaaaggt accaaaaccc
     1741 accagggtca cgctctcgcc cttggccagc gcctcagtga cgatttccac aacgccgtcg
     1801 acggcacgac cggcttcgac cttggtcatc ccggttttgc ccgccaaagc atcaatcagt
     1861 tcagtcttgt tcatagcgcg taacttttag atgaagaagt ggatgtagaa atttgggtgg
     1921 atgcagcctt gcaagcagga tctgtgggcg tcctgccttg cgtgcccata aacccgcgga
     1981 gcgaagctta cccttctccc acgagcctgt ggtgcgaatc tcacgatttt ccgcgggttc
     2041 tgacccaatt caagcgcaaa acgcatacat caaccggtcg acaggccgcg cgcctgctcc
     2101 agggcttcct cgatccgttc cacggcgatg acccgcagtc cgggaatggc ctgcttcggg
     2161 gcattggcgc ggggtaccag cgccacgctg aagcccagct tggcggattc cctgagccgt
     2221 tcctggcccc gcggcgccgg acggatctcg cccgccagcc ccacctcgcc gaagaccacc
     2281 agctcccgcg gcagcggccg gctggtgagg gaggacacga tggccagcag caccgccagg
     2341 tcggccgccg gttcggcgat cttcacgccg cccaccgcat tcacgaacac atcctgatca
     2401 aagcacacca cgccagcgtg acgatgcagc accgccagca acatggccag ccggttctgc
     2461 tctaacccga ccgagagccg ccgcggactc gggctgtggg cactgtccac cagggcctgg
     2521 atctccacca gaagcgggcg cgtaccttcc tgggtcacca gcacgcagga gccggacacc
     2581 tgctgcccgt gctgggacag gaagatcgcc gacggattgc tgacgccctt caggccccgc
     2641 tcggtcatgg cgaacacgcc cagctcgttc accgccccga agcggttctt gaaggcccgc
     2701 accagacgga aactggaatg ggtatcgccc tcgaaataca gcaccgtgtc gacgatgtgc
     2761 tccagcaccc gcgggcccgc cagggcccca tccttggtga cgtgcccgac catcaccagg
     2821 ctcgtcccgc cctgcttggc gaaacgggtg agctgggcgg cacactcgcg tacctgggcc
     2881 accgagcccg gcgccgaggt cagcgcctcg gaatacacgg tctggatcga gtccaccacc
     2941 gccacgtcgg gcttcacgtc ccgcagggtg gcaagaatcc gctccaggtt gatctcgggc
     3001 agaagttgca ggccgtgggc ctccacgtgc aggcgcaccg cccgcagggc cacctgctcg
     3061 gcggactcct cgccgctcac atacaaggcc ttgagccgcc ccgccagcag ggacagggcc
     3121 tgcagcagca gggtggattt cccgatgccc ggatcgccgc cgatcagcac gacgccgccc
     3181 ggcaccagtc cgccgcccag cacccggtcg aactcctcga tgcccgtcgg cacacgcggg
     3241 gattcccgcg gctgcacctc ggccagactc tgcagacgcc cggtgtcggc ggccagcgcg
     3301 gcaaaacgcc ggttggccgg tgcctcgggc tcgacgacgg attcgtccag ggtgttccag
     3361 gcctgacaat ggggacactg cccctgccag cgcggggccc tgccaccgca ttcgcggcag
     3421 acgtagatcg aacggggctt cgccacctca ggccccgagg tagccgaccg gcacccgcgg
     3481 cgccagggcg cagaagagct cgtagctgat ggtgcccgcc gcctccgcca cctcgtccgc
     3541 cggcatgccg gccccccaca gggtcacggg agcgccgatg ccggcctccg gcagatgggt
     3601 gagatcgcag gcgagcatgt ccatggacac ccgccccagc gtgcgcgtgc gccgcccacc
     3661 caccaggatg ggcgtgccgg tgggcgcgtg acggggatag ccgtcggcgt agccgcaggc
     3721 cacaacaccc acgcgcgtcg gcaaccgtgc ctcgaaggtg ccgccatagc ccacctgctc
     3781 gccgggacgc agttcctgca cggcgatgat ctcgctggca agcgtcatca cgggacgcag
     3841 gccgagggcc tccgcgctgt gcagcgccgg catcggcgag ctgccgtaga gcatgatgcc
     3901 tggacgcacg atctgtccgc gggcgtccgg atagcgcagc agcgcagccg aattggccag
     3961 gctggccggc agaccgagcc cttcggccac ccgatggaag cttgccagct gggcggcgac
     4021 ccccacctcg tcgtcggccc gggcgaagtg cgtcatcagg gtgatccgcc tcacacacgc
     4081 cagggcctgc aggcgctgca gggccgccgg cacccgctcg ggcgtgaagc cgaggcgatt
     4141 catgcccgtg ttgatcttga gcagcacgtc caggggccgg tccagcgcag cccgctccag
     4201 catggcgagc tgggccttgg aatggatcgc cggcgagacg cccagggcgg tcagctgcgg
     4261 cagatcggcc ggttcgaaga agccttccag gagaacgatg ggcgcgtcga tgcccgcagc
     4321 gcgcagttgc gcggcatcgg ccaggtcgag cagggcaaag ccgtcggctt ccggcgcaag
     4381 ggcccgggcc acgggcagca ggccgtgacc ataggcgttg gccttgatca ccgcataggc
     4441 ggccgccgcg cccgcatggc gacgggcgag acgatagttg tgacgcaggg cgtccaggtc
     4501 gatggacgcg cggatgggac ggggcatgat gtcaggcggg cgaaaagctg aggcgggcga
     4561 tctcggcggt gacgaaatcg gcaaaggtgt taacgagacg cgagcggaac ttgtccttca
     4621 ggacgatcac ttcgaggggg gtgtacagcc ggggcgccag gggtacgctg accagcgagc
     4681 cctcggcgat ctcctggcgg atcgccgcat gggacgcaat tgcataaccg aaaccgaggg
     4741 cggtgaggtg cttgaccgcc gcgagactgc ccagttccgc ggcgatgggg agctcttccg
     4801 gcgcgatacc cgcggacgcg aagaactggt ccgacagatc gcggatggcg ttgccgggtt
     4861 cccggtggat gaaggggtgg gacgccaggt cagtggccgt cagcgcagtg gcgcgagcga
     4921 ggggatgatt cggcgccagg atcacctgca gctcgtcctg ccccgccgca cggcgatcga
     4981 tggtgggatt ctccgagacg atctcgatga ggcccacatc cagggtgcgg tccgccacgc
     5041 ggctctcgat gagctgcgaa ttgcccaccg ccacccgcgg caccacgcgc ggatagcgct
     5101 gcttgaagcc ggccaccagc gccggcagcc agtagcctgc gatggtggtg ctggtgccca
     5161 ggtgcagcac gccggacagg tcatcggtca tctcggcgac ccgtgcttcc atctcttcac
     5221 ccagaccgag gatcttctcg gcgtaggcca tcaccagttc gccggcgggc gtgagggtga
     5281 cccgcccgtg gccgcgatcg aagaggcgcg tgttgaactg ctcttccagc tgcttgatct
     5341 ggaaggtcac ggccggttgg gtcatgaaca gggcttcggc ggcgcgggtg aaggagccct
     5401 gtcgggccac cgcgtggaat acctggagtc gtcgatcggc catggctcat aaatccggtt
     5461 tatatgcgct tatttcagca catcgacacc cgccgcgcca cagggatgca gtcgaaattc
     5521 acgaatcccc caccgcgccc cgcgctggca cgaacgggca atacgtcctt cgtggtataa
     5581 agcgcccttt cctgcgttca ccgagcttcg accgcctgac gatgcccttc ggtttctaca
     5641 tgatcatggc cgcgcagttc ttttccgcgc tggccgacaa cgcactgctg atcgcatcca
     5701 tcttcatgct ccggcagatg caggcacccc aggaatacga gccgctcctc aagctcttct
     5761 tcaccgtctc ctacgttgcg ctcgccgcct tcgtcggcgc ctttgccgac tccatgccca
     5821 agtggcgggt gatgatcatc agcaacacca tcaagatcgc tggctgctcc ctcatgttcc
     5881 tcgacgtcca tcccctcatc gcctacgcgg tggtgggcct gggcgccgcc gcctattcac
     5941 cggccaagta cggcatcctg accgaatacc tcccccatcg aaagctggtg gtggccaacg
     6001 gctggatcga gggcctcacg gtgggcgcca tcatccttgg caccctggtc ggggggatgc
     6061 tcatcaagcc tggtgtctcc caggcgctgc tgggtatcga cgtgccgtac atcgacacgg
     6121 gcatcgacac gccgggcgaa gtggcgctcg tggtggtcgc cgtgctctac ctgatcgctg
     6181 ccctgttcaa cctgaaggtg ccggatacgg gcgtggacca caagccgctg aaatccagcc
     6241 ccacatatct ggtgcacgac ttcatccact gtctcaagct gctctggcgc gacaaactcg
     6301 gccagatctc cctggccacc accaccctgt tctggggtgc cggcgccacc ctgcagttca
     6361 tcgtgatcaa gtgggccgag caggccctgc acctggatct ctccaaggcc tccatgctcc
     6421 agggcgtggt ggccatcggg gtggcgctgg gtgcggtgct ggccgccaaa ttcgtgagca
     6481 tgcgcgcctc ggtccgggtg atccccctgg gtatcgccat gggcatcatc gtcaatgtga
     6541 tgatcttcgt caccaacatg catgtggcca tggtgctgat ggtgctgatc ggcgccctgt
     6601 ccggcttctt cgtggtgccc atgaatgccc tgctccagca tcgcggccac atcctgatgg
     6661 gggcaggcca ctccatcgcg gtgcagaact tcaacgagaa cctgtcgatc ctgatcatga
     6721 cggggctcta tgccctgctg atccgcgccg atgtgtccat caacaccgtg gtggtcctct
     6781 tcggcctctt cgtggcgggc accatgttcc tggtcaagcg ccggcacgaa gccaatcagc
     6841 gcgagttcga ctcggtcgcc cagctcgacg acgtcaagca ctgaaccgtg cgccgccact
     6901 ggcggcccgt gcgccttact cgctgccgtc caggagaccg tgggcgcggg cgtgacggta
     6961 catatccacc ggcccggcca gaccggtctt gtggcgaatc gtcgtctggt aattggccac
     7021 cgtcttggcg ctgaggaaga gccgctccgc cacctcgtcc acactccgcc cggctgccag
     7081 taggcgaaag atctcaaact cccgcggtga cagcgccaga gaggccggct gcccatcgcc
     7141 cgccgacacc gcggccgcca catctggcga caggggccga ccgccgcggg ccaccgtccg
     7201 caccgcttgg atcagggcgt caggctcgct gctcttgctc agatagccgt cagcgcccgc
     7261 acgcagggcc tgacggacca tggcggcgct gtcatgcatc gaaaagatca gcacacgcag
     7321 agccgggcga tgggccttga cccgcgccag ggtctccagc ccaccccgcc ccggcatcga
     7381 caggtccagc accagcacat ccgcctcatg ctcggcgaac cagtggcata ccgcgtcgct
     7441 gtgggcaaag tcggccacca ccacgatatc ttcttccatt tccagcaact ggcggtagcc
     7501 cgtgcggacg atggcgtgat cgtccgccag cacgacacgc agcggactgc ccgcgatcat
     7561 cgcgcaagca ccggcaggcg ggcccgcagg cacaggccgg gcgacaccgc cgaggcaccc
     7621 tctgctgcat tcgcgggtcc gatctgcagc cacccgccca gggcccagac ccgctcacgc
     7681 agcgccgcga gaccgctgcc acgtcccatc gccgcgtcca gatcggcaat gccgcagccg
     7741 tcgtcgcgaa cttcccactc gatggccgca gcgtcgtccg gaacgtcgcg cacggacacg
     7801 ctcacgcagc gtgcggaggc gtggcggacg acgttggcga gggcttcctg ggtcatgcgg
     7861 tagagggtcc tggacacggc gccgggaagg gtccgatccg gcggcatcac cgcctcatgg
     7921 atctcggttc ccggaccggc cgcatcctgc cagcttttga cgagttcgtg gagaaggggc
     7981 gccagggacg ccccgtcgcc cgcccccccg gcgcagacgt cggggtgcag atcccgcagc
     8041 atgccccgca ccgacgcatc cagcgtccga caggacgtct ccagcgccgc cagctggtcg
     8101 gcgaggaccg gatcgttccc gctctggcgc cgcaggcagg ccacccggct gcgcagggcc
     8161 gtgagttcct gtccgaagct ctcctgcaca cgcagggcca ggcgcgcctg ctcgtcgtcc
     8221 tgcacggtaa gcagccgcgc gtgcagcgca cgctggcgcg tctccgccgc ggccaatccc
     8281 tcgccgagat gattgagggc cgcaccgatg ctgtccagtt cctgcaccgg cagcggcggc
     8341 acccgcacgc tgaaatcccg ctggcgcagg ctggcaatgg cctccagcag ccgacgcagt
     8401 ggcttcaagg cgtggcgcgc ggccagatac agcccggcca gcagtaccag cccataggcc
     8461 atgaagagcg ccgccatgcc gccgatattg gcggcggcct cttcctgttc gctgcgggcg
     8521 tcggggctca gggccacccg ataggcctgc ccgtcggccg tcttgaggac gacccgaccg
     8581 cctgccacgg gcgcaccttc agccgccacc aggacgcgac cgtccgcgtc ctccagctgg
     8641 aagcgcaggt ggcgcaggcg gcccgcctgc cccagttgcc gcaactcgtc gagacgggcc
     8701 gcgcggctcg ccccgtcgct ggcctgcagg gcggcgaggg cttcgagcag gcgtaccgtg
     8761 ccggcggcgc cctgcccttc gcggcggatg tccacctcgg cccgccaggc ggcgagcgcc
     8821 aggaatacgg ccaggcacac cagggtcgtc catcccaccc ggcccatgag gaggcggatc
     8881 atgtccacgg gccgcctccc ctcacccggt cagaggctgt aacgcaggcc cacccacagg
     8941 gtgcgcggcg cgcccggcgc gtaaaaggtg gaatgcacca gcgggaattc cccgcccacc
     9001 gacgcaaagg gccgggcgag aacgccaccg ctgctggaga aggccgtgga tccaagttgg
     9061 gcggccgtgg tgtagcggcg gtcgaagagg ttgccgatct gaccgaagag ctgcacgccg
     9121 cggctggcct gataggtgga attgaggttg atcaccgagt agccggccga gcgccccccg
     9181 tccaggtaat acgtgccatc cgcctgatgc tggccgttct cgttgccgcg ggccgtcgat
     9241 ccggacacgc cgaccaggtc ggcgcccacc gccaacgccg gggtcacccg gtagtccgcc
     9301 gacagtttga ggatctggcg cggaatcagc ggaatgcgat cccccgggcg gacccggatg
     9361 ttgccgtcca gcccggcgct ggcggcatcg ctggaactgt tgccgctacc cccgaggagc
     9421 tcctcgctct ggtaggtggc gtccagccag gtgtagttgg cggacagatt gagggcgccg
     9481 acgcgggtgc tggcccccag ctccacgccc tggcggcggg tcttgccgaa attcctgaag
     9541 taaccgtagc cggactggtt gtcggccaca aaaaggatgt cgtccgagtt caccgcccgg
     9601 aagacgccgg cattccagcg cgtctcggcc cccaggcggc cgcgcaggcc cgcatcccag
     9661 gtgcgcgtca ccacctggtt gaggggcgga tcgccggcca tggcgttggg cagcttgcac
     9721 ggattgtccg ggttggcaca ccccagttcg atggcggtag gtgtgcggct gccttcggtg
     9781 tagcccaggt aggcgttgag ggccggcgtc gggctgaagg tgatgccggc cgccggattg
     9841 aagcggctga agcgatgatc gccgtccagc gacgcgctgc caccgcccgg attgagagcg
     9901 tcccggttct tcacggtggt cgtgttgtag cggccggata gggtggcgtg ccatttctcg
     9961 gccagggcca gcgtgtcggt ggcaaaaacg ctccaggtgc gggtacggcc attcaggtct
    10021 acgcgcgcat cgaaggcgtt ctcggacgtc tgcgtcccgt cggcgtaggc atcgaccggc
    10081 gtgatggtgt gatccgggtt gatgtagccg aactgggccg actggcggaa gttcacctgg
    10141 ctctggtcgt aggccgcccc ggcggtgaac tggttcgggc gtccggccag ggtgccggcc
    10201 accgacagct gcccgctcag gccgaaattg cgctgggtgg tgctggtgcg gttgagcagg
    10261 ccggtgcact tctcgttggg ctcgtcgttg agcagggcgt tggcgatgca gcgccaggac
    10321 ggaaacggcg tgttggaggc gttggcgccg ctggtggggt aaccggagta acccgctgcc
    10381 gccagcgccg aacgttcggc cgcattgggc tgatagaccg actggtcgag ggcgccctcg
    10441 ttcacgtcac cgttgaaggt ggtgccggca atccgccggt agtaggcgtt gcccgacagg
    10501 atcgcgtcgt ccgacaggct gtggcggcca gtcaggttga ggaacatcga gttgttcttg
    10561 gtgatgtccg ggctggtgta gacgctgttc cagtcctgat cgaggaaacg ccgctcctgc
    10621 aggccgttgc cattgagccg gttgtccgcc acggcgaggc tcaggtcgat gtcggtggtg
    10681 gcgttctgcc agccggcctt accgaagaac tggtgcacgt ccgagggcga atgggcccgc
    10741 cagccgtcct cgtggaacag gttgccggtg acgaaccatt ccagcccctt gtcgttgaag
    10801 cccccgtgct cgaattccac cgagcggcgc ccgtaggagc cccccgtgac ctgcaccgag
    10861 gcgcccggat gggtgcgccc gtccttggtg gtgagggcca gcgccccccc cagggtattg
    10921 aggccgaaca ggggattcga gccgggcatc agcgttacgc tggaaatcgc cgagcggggg
    10981 atcatgtccc agctcaccac gtcgccgaag ggctggttga tgcgcacccc gtccaggtac
    11041 accgacagcc cctggggtgt gccggccagg ggagatgcgg tgtagccgcg gtagttcacg
    11101 tccgcctgga agggattgcc ctgaacctcg ttgatgtgca cgccgctcag gctgcggttc
    11161 atgaaggccg acagatccgg cacacggacc cggtccacgt ccttgtcggt ggcgaactgc
    11221 accggcgcgg gaatctggaa gatcggctgg tccaggcccg gcaggggcgt ctggcccacc
    11281 acgaccaccg taggggcgcg cgtggcgtcg tcggccagcg ccgggagcgc ggacgcgccc
    11341 caggcagcgg ccagggtgat cacgaggggt ttgaggggct gcgtcgggcg cgcggcaggg
    11401 tactgcatag gaagtctccg tgtttttttg cgtgcgggca gagactactc agacgccgtc
    11461 ctttgcgcta cgggagaaac tcccgcgaca cacggattca gcgccctccg aagctgacgc
    11521 cgttgacagc ccaccggcac actctatata ttgataatca ttcgcattta agaagccttc
    11581 aacaccgacg gccacgcctt tcaacccttc cctgcagccc agccagccag cacagccccc
    11641 gcggcctgtc gcaagccagc cagccccgac tggagaccga catgcccgct cactgcccca
    11701 tccgtacccc gaatccgcct gcgtccgccc tcgttcccct gggcgccctg ctcttcgggt
    11761 tcagcctccc caccgccgca gccgaaagcg ccccgaccct cgcgccggtg atcgtcacgg
    11821 accgccagga cgttcccgac gcctccaaca gctaccaggg ccagaccacc accgtcggca
    11881 agctcaagca gctgcccaag gacattcccc aggccctcac catcgtcacc gagaagctga
    11941 tggaggaccg cggccagtcc accctcaagg acgccctctc caacgtggcc ggcctcacct
    12001 tcaatgccgg cgaaggcggc cgcatcggcg acaacatgaa cctgcgcggc ttctattcct
    12061 tcggcgacct ctaccaggac ggcatccgcg acaccgccca gtacaaccgg gacaccttca
    12121 acctgcagca gatcgacgtc ctgcgcggct ccggctccat gctcttcggc cgcggccagg
    12181 ccggcggcgt gatcaaccaa gtgagcaaga cggccatgct ctacgacgag aacgaggtca
    12241 gcgccaccgt cggcagctac aacacccagc gcctcaccgc cgacctgaac cgccgcctgg
    12301 gcgacaccag cgccgtgcgc atcaacctca tgaagaccga cgcgggcagt tcccgcgacg
    12361 gcgtcacctc cgaccgggaa ggcatcgccc ccaccctgcg ctggggcatc ggcacggacg
    12421 acgagatcgc cctcagccac ttctacctga agacccacaa cgtcccggac tacggcgtgc
    12481 ccttctacca gaatcgcccc ctggacgttc ccgccagccg cttctacggc accagccggg
    12541 actacgaaga caacaccacc aacctgacca ccggcagcta ccagcatcgc ttctccgccg
    12601 acagcgccgt caagaccgtg ctgcgggccg ccagctacga ccgcgagctg tgggccgccc
    12661 aaccgcagtt acggggcagc tggtcgtcgc ccaatacgtg caccgccttt tccggcaccc
    12721 tcagcgacgc cagcacgatc tgccgctcca tcaaggcccg cggcgcccgc gagaagaccc
    12781 tcaccagcca gaccgacttc accactcgct tcaccaccgg cgccctcaag cacgaagccc
    12841 tgaccggcgt cgaattcctg cgggaagacc tgagccgctg gagccacagc ctcaccggca
    12901 tcgtcgttcc cgccaccacc gtcggcgcgc ccgaccccgg cgcaccgggt ctgggtagcg
    12961 cctacggcaa ccaggtgccg accagcccgg ccagctacac cggcaactcc cgcggcgtgt
    13021 acgcccagga caccgtcgaa ttcctgcccg gctggaagct cctcgccggc ctgcgccacg
    13081 acagcctgcg cgccgactac ctgaccaccg cggccggcgc cagcagcccc acggccaccg
    13141 ccctcggctt ctccgagatg agctaccgct cgggcctgat gttccagccc gacgacgaga
    13201 ccagctacta cctggcctgg aacgactcct tcaaccccac cgccgacctc taccagctcg
    13261 acgtgggtca gggcttcccg gccgagcgca gccgcaccac cgagatcggc gccaagtggg
    13321 aactcttcga cggcgacctg tccctgcgcg ctgccctgta ccgggccgtc aagttctggg
    13381 agcgcaacac cgacctggaa tccagcggcg gcctgctcag caagaagcgt cacaccgacg
    13441 gcgtcgaact ggaggccgcc ggccgcatca ccccgaaatg ggaagtcttc ggcggcgtcg
    13501 ccctgatgga cgcagtcatc gacgaggccg gatacaccct caacaccgcg accggcgtcg
    13561 tcacggccca caaccccaac ctggtcggca tgcgcccgcg caacgccccg ccctatacct
    13621 tcaacctgtg gagcacctac aagctcgccg gtggctggcg cgtcggggcg ggggtggagg
    13681 gcaagggcga gcgcctcgcc tatggcatcg gcaccggcac cgccgccatc acccccaacg
    13741 tcgcccccgc ctaccagcgc tgggacgcca tggtggccta cgaacagccc gcctggagcg
    13801 tgcgcctgaa cgtgctcaac ctgttcgaca cgcgctacta cgagtccatc tacgacaacg
    13861 gcggccacgt cgtgccgggc accgaacgcg ccgtccaggt caccacccgc ttcaagttct
    13921 gaacatccga cgaggtctgc ccatgctcct gcacatcccc cacgtcctgt cccgcgacgc
    13981 cgtcgccgac tgccggcgcc agctcgaagc cgccgcctgg gtcgatggca ggctcaccgc
    14041 gggcagccag tccgaacagc gcaagcacaa cctgcaactg gccgaggact cggacctcgc
    14101 ccgtgccctg cgcgacaccg tgctcgacgc cctcggccgt acccccacct tcttctccgc
    14161 ggccctgccg cgacgcatct acccgcccct cttcaactgc tacacgggcg cccacaacgc
    14221 cttcggcgac cacgtggaca acgccgtacg cacccatcgc gccacccaga cccacctgcg
    14281 caccgacctg tccttcaccc tgttcttcac cgacccggac gaatacgacg gcggcgagct
    14341 ggtcatcgag gacaccttcg gctgccagcg catcaagctg cccgccggcg acctggtgct
    14401 ctacccctcg tccagcgtgc atcgcgtcga gcccgtcacg gccggcgcgc gcatcgcctg
    14461 cttcagctgg ctggaaagca tggtccgcga gaccgagcgg cgccgcctgc tctacgacct
    14521 ggaccggcag atctgccgtc tgcgcgagac ccacggcgac gacgacactg ccgtcggtct
    14581 gaccagctgc tatcacaacc tcgtccgcat gtgggcctgc ccatgagcag cgccctcgcc
    14641 atgcccctgc cgcacccacc gcgccccggc catcccgcgc gcctgcggca gagtgccggc
    14701 atcgcagcgg cctgcgggct ccacgccctc gccggcgcgc tgatcgtgca cctcgccctc
    14761 gacaccgcag cgccgcaggt cccgccccag agcagcgtcg ccgtccgcct c
//
LOCUS       DAOQPA010000091        14566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2115_length_14566_cov_17.500034, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000091 DAOQPA010000000
VERSION     DAOQPA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_14566_cov_17.500034"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PKX01_12420"
     CDS             <1..1230
                     /locus_tag="PKX01_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HMZ76979.1"
                     /translation="PVENASFMPWLVGTALIHSLAVTEKRGSFKNWTVLLAIGAFSLS
                     LLGTFLVRSGVLSSVHAFAADPKRGIFILAFLAVVIGASLTLFAWRAPKVGLGGAFAL
                     VSRETLLLVNNVLLLTAAGAVLLGTLYPLIIDALNLGKLSVGPPYFNTVFVPLMVPVL
                     FLMAAGPVARWKQADLGEIARRLRWAFGVAVAAGVSLPFVSGGWSAGVALGLALTLWI
                     FGSAIVQIRERLKTGRPPRAFWGMHLAHVGVAVTVLGIVMVKNYESERDVRMAPGDTV
                     KAGDYVLRFKGVESVPGPNYTAARGDLDLIRDGQVVLQLQPEKRKYLSSAMPMTEAAI
                     DSGLTRDVYVSLGDPLDGEDGAWSVRVYYKPFVAWIWAGALLMALGGLLAASDRRYRL
                     TAKAARATALAGQGAAS"
     gene            1227..1817
                     /locus_tag="PKX01_12425"
     CDS             1227..1817
                     /locus_tag="PKX01_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HMZ76980.1"
                     /translation="MKRFLIPLALFLVLVGFLAIGLNRDPHEVPSPLVGKPAPDFRLE
                     VLAEPGRTFTPADMRGKVWLLNVWASWCVSCRAEHPLLVAFSKQGGVPIVGLNYKEVR
                     GDGQTDVKGLAPADEQALTRQRAAEWLARHGNPYSLSVLDMDGRVGIDYGVYGVPETY
                     LIDKKGVIRYKQIGPITPEALEQKILPLARQLEQEA"
     gene            1819..2370
                     /locus_tag="PKX01_12430"
     CDS             1819..2370
                     /locus_tag="PKX01_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HMZ76981.1"
                     /translation="MTMPSRAQRVRPAITMRGRLLATAAALLVAGSPVLAADARPLAD
                     DPIVEARMLTISEELRCLVCQNETLAASRAELAEDLRREVRSLIRQGKSDTEIKDYLV
                     TRYGDFVLYRPPVKSTTWLLWFGPFLLLAGGVAGLVAYLRRRAGRVAPPTLSAADRAA
                     AEALLAGSADAPAGSTSSPKDQA"
     gene            2367..3590
                     /gene="ccmI"
                     /locus_tag="PKX01_12435"
     CDS             2367..3590
                     /gene="ccmI"
                     /locus_tag="PKX01_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HMZ76982.1"
                     /translation="MTAFWIAAGLLTALVLALLCRPLLHRRDATGASRKALNAAIYRD
                     QMAELERDLQSGVLSRDDYAVARDELERRVLEDVAADDTPTDTRRRLPRSAVGLAVLL
                     PAAAVGLYFVLGTPAALDPARVAGTEQAPSQADIEKMVAALAAKLDKEPNNPQGWVML
                     GRSYRVMGRLDEAEKAFGRAGPVMESEPALMLELAELSADRNEGRVEGHARELLLRVL
                     KDAPENPQALVIAGTDAFFRKDFAAAIRHWEKVLALVPPDSEDATNLTAGIERARAQL
                     GQGTAAARPATATPVKDAAASAVKGRVELAPGVHAAPDDTVFIFARAFDGPRMPLAVI
                     RTKASALPMDFTLDDSLAMSPDLRLSGAAQLRIEARVSRSGDALARPGDFTASVGPVK
                     PGTRGLRLVIADPVK"
     gene            complement(3615..>5764)
                     /locus_tag="PKX01_12440"
     CDS             complement(3615..>5764)
                     /locus_tag="PKX01_12440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ76983.1"
                     /translation="QPALLSLFNWGVLVTDREGTALVSLPESMGRQGVNYGEVESIRL
                     AVSEGRAQIGRAVFGKTTGKPVIPMVVPVRDRDGRISGTLIGLTNLAAANFLDDISSN
                     RFGRRGGYLITDPEQRIFIAATDKTRVMQAGPPVGVNPVFDRYLEGYDGSGVARSSRG
                     VVELSSSKRVPSTGWLMQAVLPSEEAFAPIDEVLQRIVVASVAVGLLAAGLVLWWLRH
                     ELRPLGEAASMLGQMRDGLIPRQPLPVRRDDETGALAGAFNGLLAAIVAEEARAAEHL
                     FNQRLRAIVSRVPGIVIQYQRYDDGRYALPFASDALREICGLEASAVEHSADDFHALL
                     HADDAEAFFASRDCSAEALEPWHCEFRIRHPEAGVRWLLLDAMPEPDAAGLSWHGFVT
                     DITGLKEIESELRIAAATFETQEGIFITDAEKRIVRVNQAFTAMTGYSVEEAVGQRPS
                     LLRSGLHSADFYRHLHESLAKDGFWQGEIWNRRKNGEIYAEWVTISAVQDTAGRVTNY
                     VAAFSDITEHKKSEEQIHRLAFYDPLTQLPNRRLFYDRLEQAAVASARAGYGCALLFL
                     DMDGFKSLNDRYGHALGDELLVTVAQRLSVCVRESDTVARLGGDEFVVILEHLGDEPA
                     AAAQAEMVAQKIREALVAPYELRGRNGDTHVTFNCSASIGVSLFRGHELSREDIIKRA
                     DIAMYEAKAGGRNTIRFYTPACEVAAGLKAAAG"
     assembly_gap    5765..5864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5865..6115)
                     /locus_tag="PKX01_12445"
     CDS             complement(<5865..6115)
                     /locus_tag="PKX01_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76984.1"
                     /translation="MFAQFRRSLRARIAAGVLGGVFVTLWLTYVTLDYFLRREMEATL
                     SAQQFSVVSLLADDIDRSVRERLQALEAVAEVLQQAGRGA"
     gene            6361..6738
                     /locus_tag="PKX01_12450"
     CDS             6361..6738
                     /locus_tag="PKX01_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ76985.1"
                     /translation="MTSSTPPPDPGRPPTVRNLFADRPSAGPDEHFDTLLNHPQARIE
                     RILSHRHASPPDFWYEQPGDEWVLLVHGSATLEFDNGQRTELRPGDWLTLPAGCRHRV
                     AATGPDTVWLAVHLSRGSVSLSG"
     gene            6761..7756
                     /locus_tag="PKX01_12455"
     CDS             6761..7756
                     /locus_tag="PKX01_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ76986.1"
                     /translation="MIRRRIALLTFSALACTAAVAPVGPLVGAAHAEAPAVRTQVPGF
                     YRMALGDAEVTALYDGYIDLDTKILSNASPAEIQRLLARMFIAGEKVQTAVNTYLVNV
                     GGKLILIDTGTAKAFGPTLGFVADHLRAAGYTPEQVDVVLLTHLHPDHVNGLLGADGK
                     PLFPNAQVRVAQVESDFWLSADNTAKAPKDFQPFFKMAQAAAAPYQASGQWKPFHDES
                     EVAPGIKPVPAVGHTPGHSAYVVESKGERLLILGDTVHSYAVQFARPEVAIEFDTDKK
                     QAVATRKKLFAWAAREKLAVAGMHLPFPGLGHIRSEGKAFAWVPVEYSPLRDRAK"
     gene            complement(7885..9021)
                     /locus_tag="PKX01_12460"
     CDS             complement(7885..9021)
                     /locus_tag="PKX01_12460"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HMZ76987.1"
                     /translation="MIDDLCLLDDALDGAAECLQRWRASRPAAGVLALVAEASRDRVP
                     ALQAIARAAAMPLVGAVFPALVTATGFAVRGTWLLCLDPMPPTFLLEDVATADGVERL
                     AAAMGAHLQSPDGRAPPLMFTIFDALVPNIGTLMMTAHSRLPAPPRHAGANAGSERFQ
                     PIPCLFDATREVAGGVLGLCLPPDMAAVVRHGYPVSGTQMRATSTTGNRIDRIDGRPA
                     FTVYREVIWASFGVALTHANFYEYAVHFPLGVITMFDVLVRIPVGFTDDGAVVCVGEV
                     PPNSVLRVLEAPAAADDALLDGIVRGLNVAPGTMQTPLLTFYCAGRRMHFGAAESGRE
                     VERLRGRAGAAQIVGALSLGEIDSIEDLGFPRFHNAALVCLART"
     gene            complement(9128..10294)
                     /gene="metK"
                     /locus_tag="PKX01_12465"
     CDS             complement(9128..10294)
                     /gene="metK"
                     /locus_tag="PKX01_12465"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMZ76988.1"
                     /translation="MAKEYLFTSESVSEGHPDKVADQISDAVLDAILAQDPRGRVACE
                     TLVSTGLVVISGEITTRADINYMDIARETVKRIGYDDSEIGFDYKSCAVLTAINRQSS
                     DIAQGVNEGEGLDLDQGAGDQGLMFGYACNETPSLMPFPIYYAHRIMQRQGEVRKDGR
                     LPWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHHPDVSHAALSEAVIEEVIKPVLPKE
                     MLTPATRFLINPTGRFVIGGPNGDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDR
                     SAAYAGRYVAKNIVAAGLAERCEVQVAYAIGVARPVSLMVDTFGTGRISDEKIVELVE
                     AHFDLRPKGIIASLDLLRPIYSRTAAYGHFGRDEVDFTWETTDKAAALREAAGL"
     gene            10522..11397
                     /locus_tag="PKX01_12470"
     CDS             10522..11397
                     /locus_tag="PKX01_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ76989.1"
                     /translation="MASLFFSLLARLPLGLLQQLGAGLGWLTYALSGSYRRRLRENLE
                     RALGPEAAARVRPAAIADAGRQALELPWILLRPQADLARKMVAVSGWEHVEAAEARGG
                     GVLFITPHLGCFEITAQYFSLHKPITVLYRPPKKALLQPLIDAGRSRGNMHLAPADTA
                     GVRRLVKALRAREAVGMLPDQVPGAGEGQWVPFFGRPAYTMTLAARLSEVAGVSTLFA
                     WAERLPGGRGFHLHLRPALIPLTGDTAARCAAINREMEAQIRACPAQYLWGYNRYKRP
                     AGAPAAPPTAEVPPA"
     gene            11394..12266
                     /locus_tag="PKX01_12475"
     CDS             11394..12266
                     /locus_tag="PKX01_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HMZ76990.1"
                     /translation="MSRLLVALLWLIHWLPLSVQAPIGRGLGHLLYWLIAPRRKVALT
                     NLRLCFPELSEAERVALCKEHFALAGRSLLERGIAWFASEERVRRIVRLDNEPMLRRL
                     IEGGQPVILLAPHFLGLDLGGSRMAIAFDSVSIYARQSDPVIDRWLYHGRSRFGNQLL
                     LARDESIRASIKAMKALRPFYYLPDMDHGAEESVFVPFFGVQAATLAALPRFAKLGGA
                     VVVPCVTRMLPGGLGYVVELGEPWQDFPGPSVEGDARRMNAFLEDRIRSMPAQYYWVH
                     RRFKTRPPGEPPIY"
     gene            complement(12294..12524)
                     /locus_tag="PKX01_12480"
     CDS             complement(12294..12524)
                     /locus_tag="PKX01_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76991.1"
                     /translation="MGLKRLPAGAWLMGVPGLACLIAGALLLAGFGAELHPLLQESGA
                     GLVLIVSGVALVGSAAFPLVLAELAAKDDPTE"
     gene            complement(12527..13267)
                     /gene="trmB"
                     /locus_tag="PKX01_12485"
     CDS             complement(12527..13267)
                     /gene="trmB"
                     /locus_tag="PKX01_12485"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMZ76992.1"
                     /translation="MNDTPHIPADAQPADAPEGASAPRLTSQSIRSFVLRQGRMSEAQ
                     HRFLDEMMPRVGLAYRPAPVDLDAVFGRTAPHIVEIGFGMGASTAEIARARPDEDFVG
                     IEVHAPGVGSLCKLIDEGGLHNLRIVQHDAVEVLRDMIPEASLAGVHIYFPDPWPKKR
                     HHKRRLVQGPFVRLIASRLAPGGYLHCATDWEDYAVQMQDVLSAEPLLANTADGYAPR
                     PDYRPLTKFEQRGLRLGHGVWDLIFRRV"
     gene            complement(13343..14128)
                     /locus_tag="PKX01_12490"
     CDS             complement(13343..14128)
                     /locus_tag="PKX01_12490"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HMZ76993.1"
                     /translation="MDDALTIAGKRYSSRLLVGTGKYRDFAQTREAVLASGAQIVTVA
                     IRRTNIGQDPNAPSLLDALPPSEFTYLPNTAGCYSAEDAVRTLRLARELLDDHRLVKL
                     EVLGDPHTLYPNMPETLQAARTLVKEGFEVMVYCSDDPIQAKMLEDIGCVAVMPLASL
                     IGSGMGVLNPWNVQLVRDAVKLPLLLDAGVGTASDAAIAMELGCDGVLMNTAIALAQD
                     PVRMAAAMKKGVEAGREAFLAGRMPRKFYTASPSSPTTGLIGS"
     gene            complement(14208..14444)
                     /gene="thiS"
                     /locus_tag="PKX01_12495"
     CDS             complement(14208..14444)
                     /gene="thiS"
                     /locus_tag="PKX01_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HMZ76994.1"
                     /translation="MFTDSRPPGGAVLSLMVNGEARVLPAPATVFELLTSMGLVGKRV
                     AVERNGDIVPKGLHAGTALADGDRLEIVVAVGGG"
BASE COUNT         2241 a         5424 c         4622 g         2179 t
ORIGIN      
        1 ccggtggaga acgcctcctt catgccctgg ctggtgggca cggccctgat ccattccctc
       61 gccgtcaccg aaaagcgcgg cagcttcaag aactggaccg tgctgctcgc catcggcgcc
      121 ttctccctgt ccctgctggg caccttcctg gtccgctccg gcgtgctgag ttccgtgcac
      181 gccttcgccg ccgaccccaa gcgcggcatc ttcatcctgg ccttcctggc ggtggtcatt
      241 ggcgcctccc tcaccctctt cgcctggcgc gcccccaagg tcggcctggg cggcgccttt
      301 gcgctggtgt cgcgggagac cctgctgctc gtgaacaacg tgctgcttct caccgccgcc
      361 ggcgccgtgc tgctgggcac cctgtacccg ctgatcatcg acgccctcaa cctgggcaag
      421 ctgtcggtcg gcccgcccta cttcaacacg gtcttcgtgc ccctgatggt gcccgtgctc
      481 ttcctgatgg cagccggccc ggtggcgcgc tggaagcagg ccgatctcgg cgagatcgcc
      541 cgccgcctgc gctgggcctt tggcgtggcg gtggcggccg gcgtgagcct gcccttcgtc
      601 agcggcggct ggtcggccgg cgtcgccctg ggcctcgccc tgaccctgtg gatcttcggc
      661 tcggccatcg tgcagatccg tgagcgcctc aagaccggcc ggccaccccg cgcgttctgg
      721 ggcatgcacc tggcccacgt gggcgtggcg gtgaccgtgt tgggcatcgt catggtcaag
      781 aactacgagt cggaacggga cgtgcgcatg gcccccggcg atacggtcaa ggccggcgac
      841 tacgtgctgc gcttcaaagg tgtggaatcc gtgccgggcc ccaactacac ggccgcccgc
      901 ggtgacctgg acctgatccg cgacggccag gtggtgctcc agttgcagcc ggagaagcgc
      961 aagtacctct cctccgccat gcccatgacc gaggccgcca tcgattccgg cctgacccgc
     1021 gacgtctatg tctccctcgg cgacccgctg gacggcgagg acggcgcctg gagcgtgcgc
     1081 gtgtattaca aacccttcgt cgcatggatc tgggccggcg cgctgctgat ggccctcggc
     1141 ggcctgcttg ccgccagcga ccggcgttac cgcctcaccg ccaaggctgc ccgggccacc
     1201 gctctggccg gccagggagc cgcctcgtga aacgattcct gattcccctt gccctgttcc
     1261 tcgtgctggt gggcttcctc gccatcggcc tcaaccgcga tccccacgag gtaccctcgc
     1321 ctctggtagg caagccggca ccggacttcc gcctcgaggt gctggccgag ccgggccgga
     1381 ccttcacccc cgccgacatg cgcggcaagg tctggctgct caacgtgtgg gcctcctggt
     1441 gcgtctcgtg ccgggccgag catccgctgc tggtggcctt cagcaagcag ggcggcgtgc
     1501 ccatcgtggg cctcaactac aaggaggtac gcggtgacgg ccagaccgac gtgaagggcc
     1561 tcgcccccgc cgacgaacag gccctcaccc gccagcgcgc cgccgaatgg ctcgcgcgcc
     1621 atggcaaccc ctacagcctg tcggtcctcg acatggacgg tcgggtgggc atcgactacg
     1681 gcgtgtatgg cgtgcccgag acctacctga tcgacaagaa gggagtgatc cgctacaagc
     1741 agatcggccc catcaccccc gaggcgctcg aacagaagat cctgcccctc gcccgccagc
     1801 tggaacagga ggcctgaggt gacgatgccc tcccgcgcgc agcgcgtgcg ccccgccatc
     1861 acgatgcgcg gtcgcctcct cgcgaccgcc gccgccctgc tcgtggccgg ctcgccggtc
     1921 ctcgccgccg acgcccgccc cctcgccgac gacccgatcg tcgaggcccg catgctgacc
     1981 atctccgaag aactgcgctg cctggtctgc cagaacgaga ccctcgccgc ctcccgcgcc
     2041 gagctggccg aggatctgcg ccgcgaggtg cgcagcctga tccgccaggg caagagcgac
     2101 accgagatca aggactacct cgtgacccgc tacggtgact tcgtcctcta ccgtccgccg
     2161 gtcaaatcca ccacctggct gctgtggttc ggccccttcc tgctgctggc cggcggcgtg
     2221 gccggcctcg tcgcctatct gcggcgccgc gccggacgcg tcgccccgcc caccctgtcc
     2281 gccgccgacc gggccgccgc cgaagccctg ctcgccgggt cggccgacgc gccggccggc
     2341 tccacctcct cgccgaaaga ccaggcatga ccgccttctg gatcgctgcc gggctgctca
     2401 ccgccctcgt cctcgccctc ctgtgccgcc cgctgctgca ccgtcgcgat gccaccgggg
     2461 cctcccgcaa ggccctcaac gccgccatct accgggacca gatggccgaa ctggagcggg
     2521 atctgcagag cggcgtcctg tcccgcgacg actacgccgt cgcccgtgat gagctggagc
     2581 gccgcgtgct cgaagacgtg gccgccgacg acacgccgac cgacacccgc cgacgcctgc
     2641 cccgcagcgc cgtcggcctg gccgtgctcc tccccgccgc ggccgtgggg ctgtacttcg
     2701 tcctcggcac gcccgccgcc ctcgatcccg cccgcgtggc cggcaccgag caggcaccca
     2761 gccaggccga catcgagaag atggtcgccg cccttgccgc caagctcgac aaggagccga
     2821 acaaccccca gggctgggtc atgctcgggc gcagctaccg cgtgatgggc cgcctggatg
     2881 aggccgagaa ggcctttgga cgcgccggtc ccgtcatgga atcggaaccg gcgttgatgc
     2941 tggaactcgc cgaactctcc gccgaccgca acgagggccg cgtcgaaggc cacgcccggg
     3001 agctgctcct gcgggtgctc aaggacgctc cggagaatcc tcaggctctg gtcatcgccg
     3061 gcaccgacgc cttcttccgc aaggacttcg ccgccgcgat ccgtcactgg gaaaaagtgc
     3121 tggcactcgt gcctcccgac tccgaagacg ccaccaacct gacagccggc atcgagcgcg
     3181 cccgtgccca gctcggccag ggcacagcgg ccgcgcggcc cgccacggcg acgcccgtca
     3241 aggacgccgc ggccagcgcg gtgaaaggcc gcgtggagct cgcgccgggc gtccacgccg
     3301 cgccggacga taccgtcttc atcttcgccc gcgccttcga tggcccgcgc atgcccctgg
     3361 cggtgatccg caccaaggcc tccgcgctgc ccatggactt caccctcgac gacagcctcg
     3421 ccatgagccc ggacctgcgc ctctccggcg ccgcccagct gcgcatcgag gcccgggtgt
     3481 ccaggtccgg cgacgccctt gcccggccgg gcgacttcac cgccagcgtc ggccccgtca
     3541 agcccggcac ccgcggcctc aggctcgtca tcgccgatcc ggtgaaatag cagcccctgc
     3601 cccgcagcac gcgctcagcc agccgccgcc ttcagccccg cggccacttc gcaggccggc
     3661 gtgtagaagc ggatcgtgtt gcgtccgcca gccttggcct cgtacatggc gatgtcggcg
     3721 cgcttgatga tgtcctcccg cgacagctcg tggccgcgaa acagactcac gccgatgctc
     3781 gccgaacaat tgaaggtgac gtgcgtatcg ccgttgcgcc cccgcagctc gtaaggcgcc
     3841 accagggcct cgcggatctt ctgggccacc atttcggcct gggccgccgc cgcgggttcg
     3901 tcccccaggt gctccaggat caccacgaat tcgtcgcccc ccaggcgcgc caccgtgtcc
     3961 gactcccgca cgcagacgct cagacgctgg gccaccgtca ccagcaactc gtcgcccagc
     4021 gcgtggccat agcgatcgtt gaggctcttg aagccgtcca tgtcgaggaa cagcaaggca
     4081 cagccataac cggcgcgggc actggcgacg gccgcctgct ccaggcggtc gtagaacagg
     4141 cgccggttgg gcagttgggt gagcggatcg tagaaggcca gacggtggat ctgctcctcg
     4201 ctctttttgt gttccgtgat gtccgagaac gccgccacgt agttcgtcac gcgacccgcc
     4261 gtatcctgaa ccgcggagat cgtcacccat tcggcgtaga tctcgccgtt cttgcgtcga
     4321 ttccagatct cgccctgcca gaagccgtcc ttcgccaggc tctcatgcag atgacgatag
     4381 aaatccgcac tgtgcaggcc cgaacgcaac agggacggac gctggccgac cgcctcttcc
     4441 acggaatagc cggtcatcgc cgtgaaggcc tggttgaccc gcacgatgcg cttctccgca
     4501 tcggtgatga agatgccttc ctgggtctcg aaggtggccg cggcaatgcg caactcactc
     4561 tcgatctcct tgagcccggt gatgtcggtg acgaagccgt gccagctcag gcccgcagcg
     4621 tccggttccg gcatggcgtc caggagcagc cagcgcacgc ctgcctccgg gtggcggatc
     4681 cggaactcgc aatgccacgg ctccagcgcc tcggcagagc agtcgcggga ggcaaagaat
     4741 gcctcggcat cgtccgcatg caggagggcg tggaagtcgt ccgcggaatg ctcgacagcg
     4801 ctcgcctcca gcccgcagat ctcgcgcagg gcatcgctcg cgaagggcag cgcgtagcgg
     4861 ccgtcgtcgt accgctgata ctggatcacg atgcccggca cccgcgacac gatggcccgc
     4921 aggcgctgat tgaacagatg ctccgccgcc cgtgcctcct ccgccacgat ggccgccagc
     4981 agcccgttga aggcgcccgc cagggcaccg gtttcatcgt cgcgccggac cggcagcggc
     5041 tggcgcggaa tcagaccgtc gcgcatctgc cccagcatgc tggccgcctc gccgagaggg
     5101 cgcagctcgt gacgcagcca ccacaacacc agcccggccg ccagcagacc caccgccacc
     5161 gatgccacaa cgatgcgctg gagcacctcg tcgatgggcg caaaggcctc ttcggaaggc
     5221 agcaccgcct gcatcagcca gcccgtggac ggcacccgct tgctggatga caactccacc
     5281 accccccgcg aactgcgcgc cacccccgag ccgtcgtagc cttccagata gcggtcgaac
     5341 accgggttga cgcccaccgg cggccccgcc tgcatcaccc gtgtcttgtc ggttgcggcg
     5401 atgaagatgc gctgctcagg atcggtgatc agatagccgc cacgccgccc gaaacggttc
     5461 gaactgatgt cgtccagaaa attggctgcc gccaggttgg tgagcccgat cagggtgcca
     5521 ctgatccgcc cgtcccggtc gcgcacaggg accaccatcg gaatcaccgg cttgccggtg
     5581 gtcttgccga acacggcccg gccaatctgg gcgcgccctt cggacaccgc cagccggatc
     5641 gactccacct cgccataatt caccccctgc cgccccatgg attcaggcag gctcaccagc
     5701 gccgtcccct cccggtcggt caccagcaca ccccagttga acagggacag cagggccggc
     5761 tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgccc cgccccgcct
     5881 gctgcagcac ctcggcaacc gcctccaggg cctgcaaacg ctcgcgcacc gaacggtcga
     5941 tgtcgtcggc gagcagcgat accaccgaga actgctgggc cgacagggtc gcctccatct
     6001 cccggcggag aaaataatcc agcgtgacgt aggtgagcca cagggtcacg aagaccccgc
     6061 ccagcacacc tgccgcaatg cgcgcccgca gggaccgtcg aaactgcgca aacatcccgg
     6121 tcaccttcac gttccagctc tccggaccac tgctcctcct gctcacggcg ggccggggcc
     6181 cgcctctcgt cgtcgcggcc gatgcttgcc gaccgcgttg ttgtacattg ttccaccaca
     6241 gacgactata tgcctttcga cgtagtcgcc ctgcgggcag attccccatt ccgggcacct
     6301 gtccgggccg gatgggcccg caggcgagca cgtccgcccc ttcaacccga cgggacatcc
     6361 atgacatcga gcacgccgcc cccggatcct ggccgccccc cgacggtccg caacctcttt
     6421 gccgaccgcc cgtccgccgg cccggacgaa cacttcgaca ccctgctgaa ccatccccag
     6481 gcacgcatcg aacggatcct gtcccatcgg catgccagcc caccggactt ctggtacgag
     6541 cagccgggcg acgaatgggt ccttctcgtc cacggcagcg caaccctcga attcgacaac
     6601 gggcagcgca cggaactgcg gcccggcgac tggctcaccc tgcccgccgg ctgtcgtcac
     6661 cgggtggcgg ccaccgggcc ggacaccgtc tggctggccg tgcatctgtc acgcggatcg
     6721 gttagcctaa gcggatagat tcactcctcg ggaaaccacc atgatccgtc gtcgcatcgc
     6781 ccttctcact ttcagcgccc ttgcctgcac cgccgccgtg gcccctgtcg gcccgctggt
     6841 cggcgcggcc cacgccgagg cgccggccgt gcgcacccag gtccccggct tttaccgcat
     6901 ggccctgggc gacgccgaag tcaccgcgct ctatgacggg tatatcgacc tggacacgaa
     6961 aatcctcagc aacgcgagcc ccgccgagat ccagcgcctg ctggcgcgca tgttcatcgc
     7021 cggcgagaag gtccagaccg ccgtcaacac ctacctggtc aatgtcggcg gcaagctgat
     7081 cctcatcgac accggcacgg ccaaggcctt cggccccacc ctcggcttcg tcgcggacca
     7141 cctgcgcgcc gccggctaca cgccggagca ggtggacgtg gtgctgctca cccacctcca
     7201 cccggaccac gtcaacggcc tgctgggggc cgacggcaag cccctcttcc ccaacgccca
     7261 ggtgcgcgtc gcccaggtcg agagcgactt ctggctgtcc gccgacaaca cggccaaggc
     7321 ccccaaagac ttccaaccct tcttcaagat ggcccaggcc gccgccgcgc cttaccaggc
     7381 ctccggccag tggaagccct tccacgacga atccgaagtc gcgcccggca tcaagcctgt
     7441 ccccgcggtc ggccacacac cgggccacag tgcctatgtg gtggaatcga agggcgagcg
     7501 cctgctgatc ctcggcgaca cggtgcacag ctacgccgtc cagttcgccc ggccggaagt
     7561 ggccatcgag ttcgacaccg acaagaagca ggccgtcgcc acccgcaaga agctcttcgc
     7621 ctgggccgcc agggaaaagc tcgctgtcgc cggcatgcac ctgcccttcc ccggcctcgg
     7681 ccacatccgc agcgagggca aggccttcgc ctgggtgccg gtggagtaca gccccctgcg
     7741 cgacagggca aagtaagccc ccgcggtcgc gggcggcgaa aaaacgtcag acacagcgga
     7801 ctggaggccg gcccccggtc cgcttcaccc cgacgcaccg cccgcgatgc agccgctccg
     7861 gtgcgcgccc cacacgggcg cgcctcaggt ccgggccaga cagaccagtg ccgcgttgtg
     7921 gaagcgcgga aaacccagat cctcgatgct gtcgatctcg cccagggaca aggccccgac
     7981 gatctgtgcg gcccccgcgc gccccctcag gcgctcgacc tcgcgaccgc tttccgctgc
     8041 cccgaaatgc atccgccggc cggcgcagta gaaagtcagc aacggcgtct gcatggtccc
     8101 gggagccacg ttcaggccac ggacgatgcc atcgagcagc gcgtcgtcgg ctgccgccgg
     8161 cgcctccagc acccgcaaca ccgaattagg gggcacctcg cccacgcaga cgacggcccc
     8221 gtcatcggtg aagcccacgg ggatccgcac cagcacgtcg aacatggtga tgaccccgag
     8281 gggaaaatgg acggcgtact cgtagaaatt cgcgtgggtc agcgccacgc cgaagctcgc
     8341 ccagatcacc tcccgataca ccgtgaaggc cgggcggcca tcgatgcggt cgatacggtt
     8401 gcccgtcgtg gaggtcgcac gcatctgggt gccgctcacg ggatagccgt ggcgcaccac
     8461 cgccgccatg tccggcggca gacacagtcc cagcaccccg ccagccacct cgcgggtggc
     8521 atcgaacagg cagggaatgg gctggaagcg ctcactcccc gcattcgccc cggcatgccg
     8581 gggcggcgca gggagacggc tgtgggcggt catcatcagc gtgccgatgt tcgggaccag
     8641 ggcgtcgaag atcgtgaaca tcaacggtgg cgcacggccg tccggactct ggaggtgcgc
     8701 gcccatcgcc gccgcaagac gctccacgcc gtccgccgtc gcgacatcct ccagcaggaa
     8761 ggtcggcggc atggggtcga ggcacagcag ccacgttccg cgcaccgcaa agccggtcgc
     8821 cgtgaccagc gccgggaaga ccgccccgac cagcggcatc gccgccgcac gggcgatcgc
     8881 ctgcaaggcg gggacccgat cgcgggaagc ctcggccacc agggccagta cgccggccgc
     8941 cggccgcgac gcgcgccagc gctgcaggca ctccgccgcc ccgtcgaggg catcgtccag
     9001 caggcagagg tcgtcgatca tcgcagggcg gtcatggaag acatggcatg cagtgggtgg
     9061 cactcatgcg ctaggcggga ggggtcgcca aagcgctcgc ggcggacgaa ggccgacgca
     9121 ccgcggctta cagcccggcc gcttcccgca gggccgccgc cttgtcggtc gtctcccagg
     9181 tgaaatccac ctcgtcccgc ccgaagtgcc cgtaggccgc ggtgcgggag tagatcggcc
     9241 gcagcaaatc caggctggcg atgatgccct tgggacgcag gtcgaagtgg gcctcgacca
     9301 gttcgacgat tttctcgtcg gagatcctgc cggtgccgaa ggtgtccacc atcaggctca
     9361 ccggccgcgc cacgccgatg gcgtaggcca cctgcacctc gcagcgctcc gccagcccgg
     9421 cagcgacgat gttcttggcc acgtaacgac cggcgtaggc ggcggaacgg tccaccttgg
     9481 acggatcctt gcccgagaat gccccgccgc cgtggtgggc cgcgccgccg taggtgtcca
     9541 cgatgatctt gcgcccggtc aggccgcagt cgccgttggg cccgccgatg acgaagcgcc
     9601 cggtgggatt gatcaggaag cgggtcgccg gcgtgagcat ctccttgggc agcaccggct
     9661 tgatgacctc ctcgatcacc gcctcggaca gcgccgcgtg ggaaacgtcc ggatgatgct
     9721 gggtggacac caccacggtg tcgatggcca ccggcttgcc gtccacgtat ttgacggtga
     9781 gctggctctt ggcgtccgga cgcagccacg gcaggcggcc gtctttccgc acctcgccct
     9841 gacgctgcat gatgcggtgg gcgtagtaga tcgggaaggg catcaggctg ggcgtttcat
     9901 tgcacgcgta gccgaacatg aggccctggt cgccggcgcc ctggtccaga tccaggccct
     9961 cgccctcgtt cacgccctgg gcgatgtccg aagactggcg gttgatggcg gtcagcaccg
    10021 cgcagctctt gtaatcgaag ccgatctcgg agtcgtcata gccgatgcgc ttgacggtct
    10081 cgcgggcgat gtccatgtaa ttgatgtcgg ccctcgtggt gatctcgccg gagatcacca
    10141 ccagcccggt ggacaccagg gtctcgcagg ccactcggcc gcgcgggtcc tgggcgagga
    10201 tcgcgtccag caccgcatcg gaaatctggt cggcgacctt gtcgggatgg ccttcggaga
    10261 cggattccga agtgaagaga tattccttgg ccatgcactg ctcctgaaaa tgaaaaatcc
    10321 cctgtcctgg cgtcgtagcc gtctcggggg atctcggagc ggcgacgctt tagcagtatt
    10381 tgtcggcctg cgcacggggt gtccgcacac tgcacccgcc ctgcaagttg tcggattaac
    10441 tcggcggaat cacaattata tcttcgcgcc ttccctcgtc aattttccgc cccgcccgcg
    10501 ccgcgcgccc gaccgtttcc gatggcttcc ctgttcttct ccctcctggc ccggctgccc
    10561 ctcgggctgc tccagcagct cggcgccggg ctgggctggc tgacttacgc cctgtctggg
    10621 agctaccgtc ggcgcctgcg ggaaaatctt gagcgcgccc tcggccccga ggccgccgcg
    10681 cgcgtccggc ccgcagccat tgccgacgcc ggacgccagg ccctggagct gccctggatc
    10741 ctgctgcgcc cccaggccga cctggcccgc aagatggtgg cggtgagcgg ctgggagcac
    10801 gtggaggccg ccgaggcccg cggcggcggc gtgctcttca tcacgccgca cctgggctgc
    10861 ttcgagatca ctgcccagta cttctccctg cacaagccca tcaccgtgct ctaccggcca
    10921 cccaaaaagg ccctgctgca acccctcatc gacgccggcc gcagccgcgg caacatgcac
    10981 ctggcccccg cagatacggc cggcgtgcgc cgtctggtca aggcgctgcg cgctcgcgaa
    11041 gcggtgggca tgctgcccga ccaggtgccc ggcgccggcg aaggtcagtg ggtgcccttc
    11101 ttcggacgtc ccgcctacac catgaccctc gccgcgcgcc tgtccgaagt cgctggcgtg
    11161 agcaccctct tcgcgtgggc cgagcgcctg cccggcggac ggggcttcca cctgcacctg
    11221 cgcccggccc tgattcccct cacgggcgac accgccgcgc gctgcgccgc gatcaaccgg
    11281 gagatggaag cccagatccg cgcctgtccg gcccagtacc tgtggggcta caaccgctac
    11341 aaacgacccg ccggcgcccc cgcagcgccg cccaccgccg aggtgccccc cgcatgagcc
    11401 gtcttctcgt cgccctactg tggctgatcc actggctgcc cctgtccgtg caggcgccca
    11461 tcgggcgcgg actgggccac ctgctgtact ggctcatcgc gccgcgccgc aaggtggccc
    11521 tcaccaacct gcgcctgtgc tttccggaac tgtccgaagc cgagcgggtg gccctgtgca
    11581 aggaacactt cgccctggcc ggacgcagcc tgctggagcg cggcatcgcc tggttcgcct
    11641 ccgaggagcg ggtgcggcgc atcgtgcgcc tggacaacga gcccatgctg cgccgcctca
    11701 tcgagggcgg ccagccggtg atcctcctcg ccccccactt cctcggcctg gatctgggcg
    11761 gttcgcgcat ggccatcgcc ttcgacagcg tcagcatcta cgccaggcag agcgacccgg
    11821 tcatcgaccg ctggctctac cacggccgca gccgcttcgg aaaccagctc ctgctggcgc
    11881 gggacgagag catccgcgcg tccatcaagg cgatgaaagc cctgcgcccc ttctattacc
    11941 tgcccgacat ggaccacggc gccgaggagt ccgtgttcgt gcccttcttc ggcgtgcagg
    12001 ccgccaccct cgccgcgctg ccgcgctttg cgaagctggg cggcgccgtg gtggtgccct
    12061 gcgtcacccg catgctgccg ggcggactgg ggtatgtggt ggaactgggc gagccctggc
    12121 aggatttccc cggccccagc gtggaaggcg acgcgcggcg catgaacgcc ttcctggaag
    12181 accgcatccg cagcatgccg gcccagtact actgggtgca ccggcgcttc aagacccgtc
    12241 cgccgggcga gccgccaatc tactgagcgc gccccgccgg ccggggcgcc ggctcattcc
    12301 gtcggatcgt ccttggccgc cagctcggcc agcaccagcg gaaaggccgc gctgcccacc
    12361 agggccacgc cggacacgat cagcaccagc cccgcgccgg attcctgcaa caggggatgg
    12421 agttccgcgc caaaccccgc cagcagcagg gccccggcga tcaggcaggc aaggccggga
    12481 acgcccatca gccaggcgcc ggccggcagt cgcttgagcc ccatgctcac acccgccgga
    12541 agatcaggtc ccacaccccg tggccgaggc gcaggccgcg ctgctcgaac ttggtgaggg
    12601 gccggtaatc gggccgcggc gcgtagccgt cggcggtatt cgccaacagc ggctcggcgg
    12661 agagcacgtc ctgcatctgc acggcgtagt cctcccagtc ggtggcgcag tgcaggtagc
    12721 cgccgggcgc cagacgcgag gcgatcagcc ggacaaaggg cccctgcacc agacgccgct
    12781 tgtggtggcg cttcttgggc cagggatcgg gaaaatagat atgcacgccg gccagagacg
    12841 cttcggggat catgtcccgc agcacctcca ccgcgtcgtg ctggacgatg cgcagattgt
    12901 gcaggccgcc ctcgtcgatg agcttgcaca ggctgcccac gccaggggcg tgcacctcga
    12961 tgccgacgaa atcctcgtcc ggccgcgccc gcgcgatctc ggcggtgctg gcgcccatgc
    13021 caaaaccgat ctccacaatg tgcggcgcgg tgcgcccgaa caccgcgtcc agatccaccg
    13081 gcgccggccg gtaggccagg cccacgcggg gcatcatctc gtcgaggaaa cggtgctggg
    13141 cctcggacat gcggccctga cgcagcacga agctgcggat ggactgggag gtcaggcgcg
    13201 gcgcggaggc gccctccggc gcgtcggccg gctgggcgtc ggcggggatg tgcggcgtgt
    13261 cgttcatgga tcgtgcgggc agacgcggcg tcacgcgccg cgggtcatct ggggaaagtc
    13321 ttcggtgcgc gtgggccggc gctcagctgc cgatgaggcc ggtggtgggc gagctcgggc
    13381 tggcggtgta gaacttgcgg ggcatgcgcc cggccaggaa ggcctcgcgc ccggcctcga
    13441 cacccttctt catggcggcg gccatgcgca ccgggtcctg ggccagggca atggcggtgt
    13501 tcatcagcac cccgtcgcag cccagctcca tggcgatggc ggcgtccgac gccgtgccga
    13561 cgcccgcgtc cagcagcagc ggcagcttca ccgcgtcgcg caccagctgc acgttccacg
    13621 ggttgagcac gcccatgccg gagccaatca gcgaggccag gggcatcacc gccacacagc
    13681 cgatgtcctc cagcatcttg gcctggatcg ggtcgtcgga gcagtacacc atcacctcga
    13741 agccctcctt caccagcgtg cgcgccgcct gcagggtctc gggcatgttg gggtagaggg
    13801 tgtggggatc gcccagcact tccagcttga ccaggcggtg gtcatccagc agctcccgcg
    13861 ccaggcgcag ggtgcgcacc gcgtcctcgg ccgaataaca gccggcggtg ttgggcagat
    13921 aggtgaattc cgacggcggc agcgcgtcca gcaggctggg cgcgttcgga tcctggccga
    13981 tgttggtgcg gcggatcgcc accgtcacga tctgcgcgcc gctcgccaac accgcctcgc
    14041 gggtctgggc aaaatcccgg tacttgccgg tgcccaccag caaccgggag gaatagcgct
    14101 tgcctgcaat cgtcagtgca tcgtccatgg gaagatccgt cgcgaaaaat tctggggaat
    14161 gaagccggag aggaagaacg ccgggccgca gaccgggcgc cgcgggccta gccgccgccc
    14221 accgccacca cgatctcgag ccggtcaccg tccgccagcg cggtgccggc gtgcaggccc
    14281 ttgggcacga tgtcgccatt gcgctccacg gccacccgct tgcccaccag ccccatgctc
    14341 gtcagcagct cgaacaccgt ggccggcgcc ggcagcacac gcgcctcgcc attgaccatg
    14401 agggacagga cagcgccgcc gggcggccgg gaatcggtaa acatcggcac agtttaccac
    14461 gcccatcccg gttgcccccc ggccgccgga tgcgtacaat cctcgtcctt cgagcgggcg
    14521 tcgtataatg gtaataccct agcttcccaa gctagagccg tgggtt
//
LOCUS       DAOQPA010000092        13992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2247_length_13992_cov_20.793284, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000092 DAOQPA010000000
VERSION     DAOQPA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_13992_cov_20.793284"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKX01_12500"
     CDS             <1..786
                     /locus_tag="PKX01_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76995.1"
                     /translation="AFAALAGIILGLPTLRLRGDYLAIVTLGFGEIVRIFLNNLNHPV
                     NITNGPQGINMIDPLVLFGWDISRPLKIGDEITVNSLFLYYYVFLACVALSVMLVQRL
                     QVSRVGRAWAAIRDDELAAKAIGIDTRNMKLLAFSLGATFGGVSGGLFAAFQGFVSPE
                     SFSLMESIAVLTMIVFGGMGNLAGVIVGALALTALPEVLRHVAVPLQQSLFDRVILDP
                     EVLRMLLLSLAMIFMMLLRPAGLIPAHSRYSRPELVLGKESAR"
     gene            783..1574
                     /locus_tag="PKX01_12505"
     CDS             783..1574
                     /locus_tag="PKX01_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76996.1"
                     /translation="MITLLEARGITKRFGGLTALSDVSLTIRKGEVYGLIGPNGAGKT
                     TFFNVLTGAYTAEEGELVFNGTELPAGKPHRVVEAGIARTFQNIRLFRDMTALENVMA
                     GHHIRSRANPFAILLQTRQAREEERLITERAYELLRYVGIERFADTVSKNLSYGDQRR
                     LEIARALATEPQLLALDEPAAGMNATETGRLRDLVQTIRHDGITVLLIEHDVKLVMGL
                     CDRLAVLDFGRKIAEDVPAEVQRNEAVIAAYLGGHSTAAAVKEAS"
     gene            1571..2293
                     /locus_tag="PKX01_12510"
     CDS             1571..2293
                     /locus_tag="PKX01_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ76997.1"
                     /translation="MSDASLLELTGLRVAYGAIEAVKGIDLHLAKGELVALIGANGAG
                     KTTTLNAIAGTLPAAGGEIRYDGARINALPAHRRLRKGLALVPEGRGIFTRLTVEENL
                     RMGAYCRRDAAGVEEDMDRIFSMLPRVKERLQQVAGTLSGGEQQMVAIGRALLSRPRL
                     LLLDEPSMGLAPLVVEKIFEVIRSVAAGGVTILLVEQNAHLALEFCQRAYVMESGAVT
                     LSGTGARLLADPKVRAAYLGEL"
     gene            2376..2858
                     /locus_tag="PKX01_12515"
     CDS             2376..2858
                     /locus_tag="PKX01_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HMZ76998.1"
                     /translation="MSVLSVSASSSSLSATRPGGAGGASALSDAERRTVARMQQRDRD
                     VRAHELAHITAGGGLITRGAAYTYETGPDGQRYAVGGDVGIDVSPGRTPQETLARAEQ
                     VRRAALAPADPSAQDRQVAARADQMAFQAQQAIAAQATSGTDPTSDTPPPGSLIDVYA
                     "
     gene            complement(2849..3517)
                     /locus_tag="PKX01_12520"
     CDS             complement(2849..3517)
                     /locus_tag="PKX01_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ76999.1"
                     /translation="MNWAESIDLYCERTDASFGSEPLNALTNLAFVAVALWLVRRYGR
                     TAPRDARGLIALIAMVGLGSLVFHTVATRWAALLDVGFIAVFVLAYFQRFQCHVLGGT
                     DAAAWRGLGVFLVLAAGFAVLLRWGPPWSLNGSEIYLPPFALLGYCAWQARRRDLAAG
                     RWLMAASGLFLFSLACRVIDQALCPLWPWGTHFGWHLGNAAVLACCMEGLLRHLPGGA
                     ADQA"
     gene            3793..5757
                     /locus_tag="PKX01_12525"
     CDS             3793..5757
                     /locus_tag="PKX01_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77000.1"
                     /translation="MHTPHATRRLPRLGLALAVAVPLVLGGLAFADGSLLKSPQGKGE
                     TERNASGTGTPAATTPTRNRASSSEDGGRVTPSSSSSGSGVTTVSIVGSPTVATGSQT
                     VVTSTAAATLPRYTLLAWNDLGMHCVDGKDYSIMSILPPYNNLHAQLIDRQSGKQVTS
                     GVTLSYESLRDPSGSINTFSAGKTNFWQYVKALYGGSPAPDRGLNLSNPAVSNPTPSS
                     RPAAMSFNTVWRWYEAEGLPILPYDDTLTTAGYTKNYYPMVKVVAKNSTGTVLASTQT
                     VLPVSDEMTCVGCHASNSNRDAARPPVRGWANFTANPEKDWKKNVLRLHDDKHANDPV
                     YQAALLKLQPAASGGLALRADQGTPTLCSSCHGSNALNSPGFTVTVNGRAVKISAFTS
                     AMHTLHGKVKDPVKQVALDTIGNRDSCYQCHPGAVTKCLRGAMSDTPNLDCQSCHGNM
                     GAVGKSTRVGWLQQPNCQSCHHDGKRETVAVTDVTTGALRAVTDTRFATEPNKPATGY
                     SLFRFSVGHGKNQCEACHGSTHAEYSSKNQAWASSHDNDLLQSKAIQGYAGTLTECSV
                     CHTSVPLTTNGGPHGLHTIGQAWVNAHGDRVESSGASACKSCHGADYRGTALSQVKVA
                     KTFSHDGRQYALAAGTQVGCYTCHNGPKGD"
     gene            5973..6860
                     /locus_tag="PKX01_12530"
     CDS             5973..6860
                     /locus_tag="PKX01_12530"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HMZ77001.1"
                     /translation="MTPPSEPHGPAGGEGPSSLDPALADALRAVLAPLARLALARGVS
                     CGAVEELLKEMFVDEAMRAQGASASGRMVSRISVATGLNRREVTRLTRAERAPRAVRQ
                     PPSSEVFARWITDPALRDADGRPVELPRLPDERGSLSFETLARSVTRDVHPRSLLDEL
                     CRIGVARHDEATDRVCLLRADFVPDADRGGMLGFLGDNVGDHLNAAVANVTAAVPIHF
                     EQAVFADELSETALVAVKALVRDHWLALVHGAVPALEALIEADRQAGRAQDRRVRIGM
                     YSYAAPQTAPVGPAEESTP"
     gene            6857..8047
                     /locus_tag="PKX01_12535"
     CDS             6857..8047
                     /locus_tag="PKX01_12535"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HMZ77002.1"
                     /translation="MTTFLTRAGRHFCALLVILFALASCGGGGSGVGDLAGVGTGGTG
                     PAAVGIGPISGFGSVIVGGVRYDDSRAVVTDDEGAPRSRLDLRLGMVAEISGSADFTA
                     RTGVATAIRYGSEVLGPVTSVNTAANRFSVLGVGVDVVADTVFDERLTGVAALGMGDP
                     VEVYGYYNVATGRFTATRVDSATGASRYTLRGTVSGLDTTRKRFALAGLTIDYAAVPA
                     AQTAALANGQALRVSATRAPAGGVWVIDALTGAARASLAEGEAEIVGRIDSLTSARHF
                     SVEGVSVDATAASVSGTPALGLRVQVEGTVKNGVLQAREVEVSDDGQSETEEFDLTDV
                     IQSFDPATQRFRLRGQLVDASGTVSFEDGSRSDLANGVRIECKGSFDAALGAVRARLI
                     HFER"
     gene            complement(8063..9277)
                     /locus_tag="PKX01_12540"
                     /pseudo
     CDS             complement(8063..9277)
                     /locus_tag="PKX01_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(9304..9702)
                     /locus_tag="PKX01_12545"
     CDS             complement(9304..9702)
                     /locus_tag="PKX01_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77003.1"
                     /translation="MSVSPAPAAPRSSKKWLVSLIGFFVIVAALVAAYTWLVLSWSYS
                     NGERAGYVQKFSKKGWLCKTWEGELAIVAIPGSLPEIFRFTVRDEKVATDINAVMGKR
                     VSLTYDQHVGIPTTCFGETGYFVSQVHAVE"
     gene            complement(9721..11220)
                     /locus_tag="PKX01_12550"
     CDS             complement(9721..11220)
                     /locus_tag="PKX01_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HMZ77004.1"
                     /translation="MSLALLKSRALAGMQAPEVIVEVHLANGLPAFTLVGLPDMEVRE
                     ARDRVRAALQTSQFEFPQRRITVNLAPADLPKEGGCFDLPIALGILAASGQIPAEALA
                     GYEFAGELSLTGELRPVRGALALARQAAGSGRRLILPTANAAEAALVDGLVLLPAASL
                     LDVAAHLCGHTPLAPYAGAPLLAEVSYPDLADVKGQAPARRALEVAAAGRHSLLLFGP
                     PGTGKSMLAQRLPGLLPPMDDVEALESAAIQSVDGSFRPERWRQRPWRSPHHGASVAA
                     IVGGGSTPRPGEISLAHQGVLFLDELPEFDRRVLEALREPLENGHITVSRAARRADFP
                     ARFQLVAAMNPCPCGYLGHPDGRCRCTPDQVARYRGKLSGPLLDRIDLLLEVPALPAA
                     DLQAAPAGEPTAAVRERVAAARAVQQARQGKANADLGGAEVDRWCRPDAAGVNLLQTA
                     IARLGLSARGYHRILRVARTIADLAGRPDVGAAHVAEAVQSRRGLPGTV"
     gene            complement(11291..12562)
                     /locus_tag="PKX01_12555"
     CDS             complement(11291..12562)
                     /locus_tag="PKX01_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77005.1"
                     /translation="MIPAFRSSRSVRHLLAACVSLPLLAACAGIGPPAIDQTRLQYNE
                     ALKRTAEEQLLLNIVRLRYTDTPSSLAVTGIAAQFEVAQSLGLLPFFTAAGGDLNRSF
                     TAVLPQAQATRADRPTFSFTPLDDQEFTRKLFTPLPLEGLVYLAKTTWPISTVFRLYL
                     ENLNWVPNAQTASGPTPRQAPPINDFLEGVHALQRLQDRGDVVFGVEEQEELLGGPLP
                     ATAVTPRDVIEAARSGYVYRPDEDGRSWSLVRRFTRPTLQIAPSAVNAEATQRFLRAF
                     RLKPGLLQYAVTQEALPPFREQQPRGGLTQIDLETRSLLQALYYVSLGVDVPPAHLER
                     GVVTLTRDADGTPFDWQRVMIGLFRVRASAADARPADAHVAVRYRDHWFYVDDRDTDS
                     KSTFSLLMELARLELAGKTGPGPQLTLPVGR"
     gene            complement(12649..13224)
                     /locus_tag="PKX01_12560"
     CDS             complement(12649..13224)
                     /locus_tag="PKX01_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ77006.1"
                     /translation="MSALRCALAALLTVGGVLPVRAEPVSYSIDPNHTHVIFETDHLG
                     LSTQRGRFSRSSGKIVLDRAARSGTVDVSLDAAALDMGFAKWNENMRGENFFNVAEFP
                     AVSFRSTRLVFDGDTPVAAEGTVTLLGVSRPLTLTLQRVHCAPNPLNKRETCGADITG
                     RLKRSEFGMTRNIPSVSDEVRLIIAVEAFRD"
     gene            complement(13228..13488)
                     /locus_tag="PKX01_12565"
     CDS             complement(13228..13488)
                     /locus_tag="PKX01_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HMZ77007.1"
                     /translation="MANPKILDEIGSKVSELLANSPARDLEKNARALLASGFSKLDLV
                     TREEFEVQRDVLARTREKLAELEHRVAHLEAELAKRPPAEGV"
     gene            13762..>13992
                     /locus_tag="PKX01_12570"
     CDS             13762..>13992
                     /locus_tag="PKX01_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HMZ77008.1"
                     /translation="MRKTVLASALLASFVAAPAVSFAEDAAPAAPAFTSNVTLASEYI
                     YRGIGQTNRKPAIQGGFDYAHSSGLYVGTWASN"
BASE COUNT         2326 a         4916 c         4750 g         2000 t
ORIGIN      
        1 gccttcgccg ccctggccgg catcatcctc ggcctgccca ccctgcgcct gcgcggcgac
       61 tatctggcca tcgtcactct gggtttcggc gagatcgtgc gcatcttcct caacaacctg
      121 aaccacccgg tgaacatcac caacggtcct cagggcatca acatgatcga cccgctggtg
      181 ctgttcggct gggacatctc acgcccgctg aagatcggcg acgagatcac ggtcaattcc
      241 ctgttcctgt actactacgt cttcctcgcc tgtgtggccc tgtcggtgat gctggtgcag
      301 cgtctgcagg tgtcccgggt cggccgcgcc tgggcggcga tccgcgacga cgagctggcg
      361 gccaaggcca tcggcatcga cacccgcaac atgaagctcc tcgccttctc cctcggcgcc
      421 actttcggcg gcgtctcggg cgggctgttt gccgccttcc agggcttcgt ctcgccggag
      481 agcttctccc tgatggaatc catcgcggtg ctgaccatga tcgtttttgg cggcatgggc
      541 aacctggccg gcgtcatcgt cggcgcgctg gccctcaccg ccctgccgga agtcctccgc
      601 cacgtggccg tgcccctgca gcagtccctc ttcgaccggg tgatcctcga tcccgaggtg
      661 ctgcgcatgc tgctcctgtc cctggccatg atcttcatga tgctgctgcg cccggccggc
      721 ctgatccccg cccacagccg ctattcgcgc cccgagctgg tgctgggcaa ggagtcggcg
      781 cggtgatcac cctcctcgaa gcccgcggca tcaccaagcg cttcggcggc cttaccgccc
      841 tgtcggacgt gtccctgacc atccgcaagg gcgaggtcta cggcctcatc ggccccaacg
      901 gcgctggcaa gaccaccttt ttcaacgtcc tcaccggcgc ctacaccgcc gaagaaggcg
      961 agctggtctt caacggcacc gagctgcccg ccggcaagcc ccaccgcgtg gtcgaggccg
     1021 gcatcgcccg caccttccag aacatccgcc tgttccgcga catgaccgcc ctcgagaacg
     1081 tcatggccgg ccaccacatc cgctcccgcg ccaacccctt cgccatcctg ctgcagaccc
     1141 gccaggcccg cgaggaggaa cgcctgatca ccgaacgggc ctacgagctg ctgcgctacg
     1201 tgggcatcga acgctttgcc gacaccgtgt cgaagaacct ctcctacggc gaccagcgcc
     1261 gcctggagat cgcccgcgcc ctcgccaccg agccccagct cctcgccctc gacgaaccgg
     1321 ccgccggcat gaacgccacc gagaccggcc gcctgcggga tctcgtccag accatccgcc
     1381 acgatggcat caccgtgctc ctcatcgagc acgacgtgaa gctcgtcatg ggcctgtgcg
     1441 accgccttgc cgtgctcgac ttcggccgca agatcgccga ggacgtgccc gccgaggtcc
     1501 agcgcaacga agccgtcatc gccgcctatc tgggcggcca cagcacggcc gcggccgtga
     1561 aggaggcgtc atgagcgacg cgtccctcct cgaactgacc ggcctgcggg tcgcctacgg
     1621 tgccatcgag gccgtcaagg gtatcgacct gcacctcgcc aagggcgagc tggtggccct
     1681 gatcggcgcc aacggcgccg gcaagaccac caccctcaac gccatcgccg gcaccctgcc
     1741 cgccgcgggc ggcgagatcc gctacgacgg cgcccgcatc aacgcccttc ccgcccacag
     1801 gcgcctgcgc aagggcctcg ccctggtgcc ggaagggcgc ggcatcttca cccgcctcac
     1861 ggtcgaggag aacctgcgca tgggcgccta ctgccgccgc gacgcggccg gcgtggaaga
     1921 ggacatggac cgtatcttca gcatgttgcc gcgggtcaag gagcgcctcc agcaggtggc
     1981 cggcaccctc tccggcggcg agcagcagat ggtcgccatc ggccgcgccc tgctgtcgcg
     2041 cccgcgcctg ctgttgttgg acgagccctc catgggcctc gcgcccctgg tggtggaaaa
     2101 gatcttcgag gtcatccgct ccgtcgccgc gggcggcgtc accatcctgc tggtggagca
     2161 gaacgcccac ctggcgctgg aattctgcca gcgcgcctac gtgatggaat ccggcgcggt
     2221 caccctgagc ggcaccggcg cccggctgct ggccgacccc aaggtccgcg ccgcctacct
     2281 gggcgagctc tgagcgcctg ccgcgcagcc cggcggaccc tgtcgcgccg ggccaaggcc
     2341 ggctaggctg aagcatccgc gacggagttg cctccatgtc cgtcctatcc gtcagcgcct
     2401 cgtcgagttc cctgtccgct acccgtccgg gcggcgcggg gggcgcgtct gcgctgagcg
     2461 acgccgaacg ccgcaccgtc gcccgaatgc agcagcgcga ccgggatgta cgcgcccacg
     2521 agctggccca catcaccgcc ggtggcggtt tgatcacccg cggcgccgcc tacacctacg
     2581 agaccggccc ggacggccag cgctacgcgg tgggcggcga tgtgggcatc gacgtgtcgc
     2641 cggggcgcac accccaggaa accctggccc gcgccgagca ggtccggcgc gccgccctcg
     2701 ccccggcgga cccctcggcc caggatcgtc aggtcgccgc ccgcgctgac cagatggcct
     2761 tccaggccca gcaggccatc gccgcccagg ccacatctgg caccgatccg accagcgata
     2821 ccccgccgcc gggcagcctg attgacgttt acgcctgatc cgccgccccg cccggcaggt
     2881 ggcgcaacag cccctccatg cagcaggcca gcaccgccgc gttgcccaga tgccagccga
     2941 agtgcgtgcc ccacggccag agtggacaca gggcctgatc gatgacccgg cacgccaggc
     3001 tgaacaggaa caatccgctc gccgccatca gccaccgacc ggctgccagg tcacggcggc
     3061 gcgcctgcca cgcgcagtag cccagcagcg caaagggcgg cagatagatc tccgaaccgt
     3121 tcaatgacca gggcggcccc caccgcagca gcactgcaaa acccgccgcc agcaccagga
     3181 acacccccag cccgcgccac gccgcagcgt ccgtgccgcc cagcacatgg cattgaaagc
     3241 gctggaaata cgccagcacg aagacggcaa tgaagcccac atccagcagc gccgcccaac
     3301 gtgtggccac cgtgtggaag accaggctgc ccagcccgac catggcgatc agggcgatca
     3361 ggccacgcgc atcccgcggc gcggtccgcc cgtagcgccg caccagccac agggccacgg
     3421 ccacgaatgc caggttggtg agggcattga gcggctccga cccgaaactg gcgtcggtgc
     3481 gctcgcaata gaggtcgata ctttcggccc agttcatgaa acgcctcggc ggggacggga
     3541 aatcggctcg ccattatgcc gtcggtgcgt gcgcgacgac ggtcggtgtc ggtcggctca
     3601 atgcacggtg ggacggcagt gcgccgctgg cgatgcagga tcaagtcgat tcggcttgaa
     3661 atgtgcaatt cacacattta atataagctc atattcggca gttcattcgt ctggccgggt
     3721 cattgcaggc ggagcctgca cggggcgttc cgacggaacg ccgttcgtta tcgatctgaa
     3781 tgggacatct ccatgcatac tccgcacgca acgcgccgcc tgccacgcct gggccttgcc
     3841 ctggccgtcg cggtgcccct ggtgctcggc ggcctcgcct ttgccgacgg cagtctcctc
     3901 aagtcgccgc agggcaaggg cgaaacggaa cgcaacgcca gcggcaccgg cacgccggct
     3961 gccaccacgc cgacgcgcaa ccgcgcgtca tccagtgaag acggcgggcg cgtcacgccc
     4021 tcgtcgagca gtagcggaag cggcgtgacg acggtgtcca tcgtcggcag tccgaccgtc
     4081 gccaccggca gccagaccgt cgtgaccagc acggcggcgg ccaccctgcc gcgttacacc
     4141 ctgctggcct ggaacgatct gggcatgcac tgcgtggacg gcaaggacta ctccatcatg
     4201 tccatcctgc cgccctacaa caacctgcac gcccaactca tcgaccggca gagcggcaaa
     4261 caggtcacct ccggcgtcac cctgagctac gaatccctgc gcgatccgtc cggctccatc
     4321 aacaccttct cggccggcaa gaccaatttc tggcagtacg tgaaggcctt gtacgggggc
     4381 tcgccggcgc cggaccgggg tctcaacctg agcaatcccg ccgtctccaa tcccacgccc
     4441 agcagccgcc cggcggccat gagcttcaac acggtgtggc gctggtacga agcggagggc
     4501 ctgcccatcc tgccctacga cgacaccctg acgaccgccg gctacaccaa gaattactac
     4561 ccgatggtca aggtggtggc gaagaacagc actggcacgg tgctcgccag cacccagacc
     4621 gtgctgccgg tctccgacga gatgacctgc gtcggctgcc acgccagcaa ttccaaccgg
     4681 gacgccgcca ggccacccgt gcggggctgg gccaacttca ccgccaaccc ggaaaaggac
     4741 tggaagaaga acgtcctgcg cctgcacgac gacaaacacg ccaacgaccc ggtctaccag
     4801 gcggccctgc tgaagctcca gcccgccgcc agcggcggcc tggccctgcg cgccgaccag
     4861 ggcacgccga ccctgtgctc gtcctgtcac ggctccaacg cgcttaattc gccgggcttc
     4921 accgtgaccg tgaatggccg cgccgtgaag atctctgcct tcacctcggc catgcacacc
     4981 ctgcacggca aggtgaagga cccggtcaag caggtggccc tcgacacgat tggcaaccgg
     5041 gattcctgtt accagtgcca cccgggcgcc gtcaccaagt gcctgcgcgg ggccatgagc
     5101 gacacaccga acctggactg ccagagctgc cacggcaaca tgggcgccgt cggcaaatcc
     5161 acccgagtgg gctggctgca gcaacccaac tgccagtcct gtcaccatga cggcaaacgc
     5221 gagacggtgg ctgtcaccga tgtcaccacc ggcgccctgc gcgcggtgac cgacacgcgc
     5281 ttcgccaccg agcccaacaa gccggccacc ggctattccc tgttccgctt cagcgtcggc
     5341 cacggcaaga accagtgcga agcctgccac ggctccaccc acgcggagta ttcgtccaag
     5401 aatcaggcct gggcctcgtc ccacgacaac gacctgctcc agtccaaggc gatccagggc
     5461 tacgccggca ccctcaccga atgcagcgtg tgccacacca gcgtgcccct caccaccaat
     5521 ggcggccccc atggcctgca tacgattggg caggcctggg tgaatgccca cggcgaccgg
     5581 gtcgaatcca gcggcgccag cgcctgcaag agctgccacg gcgcggacta ccggggcacc
     5641 gccctgtccc aggtgaaagt cgccaagacc ttcagccacg acggcaggca atacgcgctg
     5701 gcggccggca cccaggtcgg ctgttacacc tgccacaacg gaccgaaggg cgactgaccg
     5761 caaagtccac cgtccaggcc gggggcggca gcgtccccgg cccgatctgg gggatccggc
     5821 gggagccgga aagccgtgcc gtgcctgcgc tcggggcgca atgggtcata atgagaacga
     5881 ttctccggac cgcaggtcca ccgttctccc agcttgaaat gtgcagatcg cacattcaca
     5941 atgtcccctg caatgatccc caaggcggtg tcatgacgcc cccctccgag ccccacggtc
     6001 ctgccggcgg cgagggccca tcgtccctcg atccggccct ggccgatgcc ctgcgcgccg
     6061 tgctggcgcc cctggcacgc ttggcgctcg cccgcggcgt gtcctgcggg gccgtggaag
     6121 aactgctcaa ggagatgttc gtggacgaag ccatgcgcgc ccaaggcgcg tcggcatccg
     6181 ggcggatggt cagtcgcatc agtgtggcca ccggcctcaa ccggcgcgag gtgacacgtc
     6241 tgacccgcgc cgagcgggcc cctcgcgccg tccgccaacc gccgagctcg gaagtcttcg
     6301 cccgctggat caccgacccg gcgctgcgcg atgcagacgg ccggcccgtc gagcttcccc
     6361 gcctgcccga cgaacgcggc agcctcagtt tcgaaaccct ggcccgctcg gtcacccgcg
     6421 atgtccatcc gcgcagtctg ctggacgaac tgtgccgcat cggggtcgcc cgccacgacg
     6481 aggcgaccga ccgggtctgc ctgctgcgtg ccgacttcgt acccgatgcg gaccggggcg
     6541 gcatgctcgg cttcctgggc gacaacgtgg gcgatcacct gaatgcggcg gtggccaacg
     6601 tgaccgccgc cgtgccgatt cacttcgaac aggcggtgtt tgccgacgag ctgtcggaga
     6661 cggccctcgt cgcggtcaag gccttggtcc gggaccactg gctggcgctg gtccacggcg
     6721 ccgtgccggc cctggaagcc ctcatcgagg ctgaccgcca ggccggccgc gcccaggacc
     6781 ggcgggtccg catcggcatg tacagctacg ccgcaccaca gaccgcgccg gttggcccgg
     6841 cggaagaatc gacaccatga cgacttttct cacccgtgcc ggacggcact tctgcgcgct
     6901 gctggtcatt ctgttcgcgc tggcctcctg cgggggcggc ggcagcggcg tcggcgatct
     6961 ggccggcgtg ggcaccggcg gcacgggtcc ggccgccgtg gggatcggac cgatcagcgg
     7021 cttcggctcg gtgatcgtcg gcggtgtgcg ctacgacgac agtcgtgcgg tcgtcaccga
     7081 cgatgagggc gcgccccgca gtcgcctgga cctgcgcctc gggatggtcg ccgagatcag
     7141 cggcagcgcc gatttcaccg cgcgcaccgg cgtggccacg gccatccgct acggcagcga
     7201 agtactcggg ccggtgacct cagtgaatac cgcggcgaat cgcttcagcg tcctgggcgt
     7261 cggcgtggac gtcgtggccg acaccgtatt cgacgagcgc ctgaccggcg tggcagcgct
     7321 ggggatgggc gacccggtgg aggtgtatgg ctattacaac gtggccaccg gccgcttcac
     7381 cgccacccgc gtggacagtg ccaccggagc gtcccgctac acactgcggg gcaccgtgag
     7441 cgggctcgac acgacgcgca agcgctttgc gctggccggt ctgacgatcg actacgccgc
     7501 tgttccggcg gcgcagaccg cggccctggc caacggacag gccctgcggg tgagtgcgac
     7561 gcgcgccccc gcgggcggcg tgtgggtcat cgacgccctg acgggcgccg cccgggccag
     7621 tctggcagag ggcgaggccg agatcgtcgg gcgcatcgac agcctgacct cggcacgaca
     7681 cttcagcgtg gagggcgtga gcgtggatgc cacggcggcg agcgtcagtg gtacgccggc
     7741 cctcggcctg cgcgtgcagg tggaaggcac cgtgaagaac ggcgtccttc aggccaggga
     7801 agtggaggtg tcggacgacg gccagagcga gacggaggaa ttcgacctga ccgacgtgat
     7861 ccagtccttc gacccggcca cccagcgttt ccgcctgcgt ggccagctcg tggatgcgag
     7921 cggaaccgta agcttcgaag acggcagccg cagcgacctg gccaacggcg tgcgcatcga
     7981 gtgcaagggc agcttcgatg ccgcgctggg cgccgtgcgg gcgcgtctca tccacttcga
     8041 gcgctgaagt ggagcggcca cctcaggcgc cgggcttcgg ggcctgaagc cgcgtccaca
     8101 gcgccacccc caccagcccc agggccatga agccgagcat gccgaaggcc aggcccggca
     8161 cggtccgcca cagggcgggc gcgaccaggc tggccgatac cgcgctggtc atcatctgca
     8221 ggaagctctg gcagctcgcc gccaggcccc ggttggccgg aaagtactcc agcgccagca
     8281 gggtcaggct gggcatggcc acggccatgc cgaagttgta gaccatcacc ggcagcaccg
     8341 agaccgggat gcccggcgcc aggagcaggc tcacgccgac gttcaaggcg caggcgaggg
     8401 ccatcacggc gtaaccgagg gccaccgtgc gggtctggct gaggcgcccg gcgagccgtg
     8461 ccgagagggc cgaaccgctc atcatgccca ccacgccggg gatgaagagc cagccgaact
     8521 cccgctccga caggcccagg tgagtcatca ggaacaccgg cgccgacagc acgtagatga
     8581 agaatccatt gaagttgaac cccaccgcca gagtcaacag caggaaaccg gcatgacgga
     8641 aggcgtcccg gtagctgcgc gcgaggctgg ccggatgcag cggacgacgc tcggacgggg
     8701 cgagggtttc cggcaggccg acggcggtga gcagggccag caccaggccg agcacggaga
     8761 gaaagacgaa caccgcgcgc cagccgaaca ggctgaagat ccagccaccg aggatgggcg
     8821 ccagggccgg accgagggca aaggccatcg ccacgtggct catcaggcgc tgggcgcggg
     8881 ggccttcgtg cagatcgcgc accacggcgc ggccgatcac catgccggcg ccagccgtga
     8941 tcccctgcag cgcccgtccg gccatcagca tctccaggct gctggcgaag acgcacacca
     9001 gcgacgccag cccgaacagc agcatggaac cgacgatcac cggcttgcgc ccccaggcat
     9061 ccgacagggc tccatgccac agcaccatca cgcccatggg cagcatgtag gcggtgaggg
     9121 tgagctggac ctgctccggc gtggcgtgga gttccgcccc gatggcgtga aaggccggca
     9181 ggtaggtgtc gatggagaaa gggccgacgg tggcgagggc tgcgagcagc gccgccagct
     9241 ggacgtaggg catggaagac cttgcggggc gcgccggggc ccgggcgcgc gacgggagcc
     9301 ggcttactcg acggcgtgaa cctggctcac gaaatagccg gtctcaccga agcaggtggt
     9361 ggggatgccc acgtgctgat cgtaggtcag ggacacgcgc ttgcccatca ccgcgttgat
     9421 gtcggtggcg accttctcgt cgcggaccgt gaagcggaag atctcgggca ggctgccggg
     9481 aatggcgacg atggccagct cgccttccca ggttttgcac agccagccct tcttggagaa
     9541 cttctgcacg tagccggcgc gttcgccgtt ggagtaactc cagctgagca cgagccaggt
     9601 gtaagccgcc acaagggcgg caacgatcac aaaaaatccg atcagcgaga ccagccattt
     9661 cttgctgctg cgcggtgcag ccggtgcggg ggagacgctc actttcagct ccaaggggga
     9721 tcagacggtt ccgggcagtc cgcggcggct ctgcaccgct tcggccacgt gggcggcacc
     9781 cacgtcgggg cggcccgcca ggtcggcgat tgtacgcgcg acccgcaaga tgcgatggta
     9841 cccccgcgcg gacaagccga gccgggcgat ggcggtctgt aacagattca cgccggcggc
     9901 gtccggccgg caccagcggt ccacctcggc cccgcccagg tcggcattgg ccttgccctg
     9961 tcgggcctgc tgcacggccc gtgcggcggc gacccgctcg cgcacggcgg cagtgggctc
    10021 gccggccggt gcggcctgca ggtcggcggc cggcagggcg gggacttcca ggagcaggtc
    10081 gatgcggtcc agcaaggggc cggagagctt gccgcgatag cgcgcgacct ggtccggcgt
    10141 gcaccggcag cggccgtcgg ggtgccccag gtagccgcag gggcagggat tcatcgccgc
    10201 cacgagctgg aagcgggcgg gaaagtccgc ccgccgcgcg gcgcgggaga cggtgatgtg
    10261 gccgttctcg aggggctcgc ggagggcttc gaggacgcgc cggtcgaatt cgggcagctc
    10321 gtcgaggaac agcacgccct ggtgggcgag gctgatctcg ccggggcgcg gggtactgcc
    10381 gccgccgaca atggcggcca ccgaggcgcc gtgatgggga ctgcgccacg gccgctggcg
    10441 ccagcgctcg ggccggaagc tgccatccac cgactggatc gcggcgctct ccagcgcctc
    10501 gacgtcgtcc atgggcggca gcaggccggg caggcgctgg gccagcatgg atttgccggt
    10561 gcccggcgga ccgaacagga gcagggaatg gcgcccggcg gcggccactt cgagggcgcg
    10621 acgcgcaggt gcctggcctt tcacgtcggc caggtcgggg tagctgacct cggccagcag
    10681 cggcgcgccg gcgtagggcg ccagcggcgt gtggccgcac aggtgcgccg ccacgtccag
    10741 cagactggcc gccggcagca ggacaaggcc gtccaccagg gccgcctcgg cggcgttggc
    10801 agtgggcagg atcaggcgcc gaccgctgcc cgcggcctga cgggccaggg ccagagcgcc
    10861 acgcaccggg cgcagttcgc cggtaaggga cagctcgccg gcgaattcgt acccggccag
    10921 ggcctcggcg gggatctggc cggaggcggc gaggatgccc agggcaatgg gcaggtcgaa
    10981 acagccgcct tccttgggca gatcggcggg ggccagattg acggtgatgc gccgctgggg
    11041 aaattcgaac tggctggtct gcagcgcggc gcggacccgg tcgcgggcct cgcgcacctc
    11101 catgtcgggc aggccgacca gggtgaaggc gggcaggccg ttggccaggt gcacctcgac
    11161 gatcacctcg ggggcctgca tgccggccag ggcgcggctc ttcaggagtg ccagggacat
    11221 gatccggatc gcatgcaaag ccgtgagtgt agcctgtacg gacgtaccgt gggatgcccg
    11281 tcgcatggtt tcagcggccg accggcaggg tgagctgggg accggggccg gtcttgccgg
    11341 cgagctccag gcgggccagc tccatcagca gggagaaggt ggatttgctg tcggtgtccc
    11401 gatcatccac gtaaaaccag tggtcgcggt agcgcaccgc cacgtgggcg tccgccggac
    11461 gggcgtcggc ggcgctggcg cggacgcgga acaggccgat catcacccgc tgccagtcga
    11521 agggcgtgcc gtccgcgtcg cgggtgaggg tgacgacgcc ccgttccaga tgcgcgggcg
    11581 gcacgtctac gccgaggctc acgtaataca gggcctggag cagggatcgg gtttccaggt
    11641 caatctgggt gaggccaccg cggggctgct gttcccggaa gggtggcagc gcctcctggg
    11701 tgacggcgta ctgcaggagg cccggcttga gccggaaagc acgcaggaag cgctgcgtcg
    11761 cctccgcgtt cacggccgac ggggcgatct gcagggtcgg gcgggtgaat ctgcggacca
    11821 ggctccagct gcggccgtcc tcatcggggc ggtacacgta tcccgagcgg gccgcctcga
    11881 tcacgtcgcg aggcgtcacc gcggtggcgg gcagcgggcc gccgagcagt tcttcctgtt
    11941 cttccacgcc gaagacgacg tcgccccgat cctgcagacg ctgcaaggcg tgcacgccct
    12001 cgagaaagtc gttgatgggg ggcgcctggc gcggcgtggg gccgctggcc gtctgggcgt
    12061 tgggcaccca gttgaggttt tccagataga ggcggaacac cgtggagatg ggccaggtgg
    12121 tcttggccag atagaccaga ccttcaaggg gaaggggcgt aaacagcttg cgcgtgaact
    12181 cctggtcgtc cagaggcgtg aagctgaagg tgggccggtc ggcgcgggtg gcctgggcct
    12241 ggggcagcac ggcggtgaag ctgcggttga ggtcaccgcc ggcggccgtg aagaagggca
    12301 gcaggccgag gctctgggcc acctcgaact gggcggcgat gccggtcacg gcaaggctgc
    12361 tgggcgtgtc ggtgtagcgc agtcggacga tgttgaggag gagctgctcc tccgcggtgc
    12421 gcttgagggc ttcgttgtac tgcaggcggg tctggtcgat ggccggcgga ccgatgccgg
    12481 cacaggcggc cagcaacggc agggacacgc aggcggccag gaggtggcgg acagaccggc
    12541 tggagcggaa cgcggggatc atggcacgag tgtgccgccc caggatgacg gggccgtgac
    12601 cgggaccgcc catcctccgg gaaggccggg caccacgccc ggcgggaatc agtcgcggaa
    12661 ggcttcgacg gcgatgatca ggcgcacttc gtcgctgacg gacggaatgt tgcgggtcat
    12721 gccgaactcg gaacgcttca gccggccggt gatgtcggcg ccacaggtct cccgcttgtt
    12781 gagcgggttg ggcgcacagt ggacgcgctg cagcgtgagc gtgaggggac gcgacacacc
    12841 cagcagggtc accgtgccct cggctgccac cggcgtgtcg ccgtcaaaca ccaggcgcgt
    12901 ggaccggaag ctcacggcgg gaaactcggc gacgttgaag aagttctcgc cgcgcatgtt
    12961 ctcgttccac ttggcgaagc ccatgtccag ggccgccgca tcgagactga catccaccgt
    13021 gccgctgcgg gcggcccggt cgagcacgat cttgccgctg ctgcggctga agcggccacg
    13081 ctgggtggac aggcccaggt ggtcggtctc gaagatcacg tgggtgtggt tcggatcgat
    13141 gctgtaggac accggctccg cccgcacggg cagaaccccg ccgacggtca ggagcgcggc
    13201 cagcgcacaa cgcagggccg acatgtttca gacgccttcg gcaggcggac gcttggccag
    13261 ctcggcctcc aggtgggcga cccggtgttc gagttcggca agcttttcgc gggtacgcgc
    13321 cagcacgtcg cgctggacct cgaattcctc gcgggtcacc agatcgagct tggaaaagcc
    13381 gctggcgagc agggcacggg catttttctc cagatcgcgg gccggactgt tagcaagcag
    13441 ctcagacact ttggagccga tttcgtcgag gatcttcgga ttggccatag caggactcct
    13501 gtacacgata gagggcattc cgggcgaccc ggagcaaggt ccgagtgtag caccgggtct
    13561 gcgcgccgcg acggcatgcc catgcacccg aatggcgcac cgacttggtg caacattcca
    13621 acttggtgcg cacgataaaa attgcgcacc gcaataggtc acgaaaaaca agtttaactt
    13681 actgtttaca aaagaattaa tgtttttggc acatgctttg ctatacagtt ctgccaactt
    13741 tgcaaacagg agattcctac catgcgcaag accgtccttg cttccgccct gctcgcttcc
    13801 tttgtggccg cccccgccgt cagctttgcc gaagacgccg cgcctgccgc gcccgccttc
    13861 accagcaacg tgaccctggc gtccgagtac atctaccgcg gcatcggcca gaccaaccgc
    13921 aagcccgcca tccagggcgg cttcgactac gcccattcca gcggcctgta cgtcggcacc
    13981 tgggcgtcca ac
//
LOCUS       DAOQPA010000093        13713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2308_length_13713_cov_18.952043, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000093 DAOQPA010000000
VERSION     DAOQPA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_13713_cov_18.952043"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="hisH"
                     /locus_tag="PKX01_12575"
     CDS             complement(<1..574)
                     /gene="hisH"
                     /locus_tag="PKX01_12575"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMZ77009.1"
                     /translation="MSSVAIIDYGMGNLRSVAKAIEHVATGATVVVTADPAAVLAAER
                     VVFPGQGAMPDCMRELDARGLRPAVLEAARSKPFLGICIGQQMLFDHSDEGDVPGLGL
                     FRGHVRRFPSDARMTAPDGGRLKVPHMGWNEVWQKQAHPLWAGIPDGSRFYYVHSYYV
                     DPADPAVIAATTEYGIPFTGAVAQANIFAVQ"
     gene            complement(576..1163)
                     /gene="hisB"
                     /locus_tag="PKX01_12580"
     CDS             complement(576..1163)
                     /gene="hisB"
                     /locus_tag="PKX01_12580"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HMZ77010.1"
                     /translation="MRQAEISRDTLETQIRVRLDLDGSGQSRLDTGIGFFDHMLDQIA
                     RHGCFDLEIAAKGDLHIDGHHTVEDVGIALGQAFARAIGDKKGLTRYGHAYVPLDEAL
                     SRVVVDLSGRPGLEFDVPFTAAAIGALDTQLVYEFFQGFVNHAGVSLHVDNLKGRNAH
                     HQCETVFKAFGRALRMAVTPDPRQAGVVPSTKGAL"
     gene            1273..2364
                     /gene="hisC"
                     /locus_tag="PKX01_12585"
     CDS             1273..2364
                     /gene="hisC"
                     /locus_tag="PKX01_12585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMZ77011.1"
                     /translation="MSRFWSPVVHDLTPYVPGEQPKLANLVKLNTNENPYPPSPRVVD
                     AIRAELGEDGDHLKLYPDPTGSALKAAVSRQFGVPAEWVFVGNSSDEVLAHTFQGLLK
                     HDAPLLFPDITYSFYPVYCGLYGIPFETVPLAEDFSIRVDDYLVRGPAAVGGVIFPNP
                     NAPTGRLLPVAEIARLAAGLPDSPVVVDEAYVDFGGHDAIPSAVALVAEYPNILVTQT
                     LSKSRSLAGLRVGFAIGQPELVEALTRVKDSFNSYPLDRLALVGACAALDDTEWFHCT
                     RHQVIASREALSASLTNLGFEVLPSAANFVFARHPAHDAALLSAALRARSIIVRHFRA
                     PRIDQFLRITVGTDAQCAQLVTALGEILG"
     gene            complement(2446..3762)
                     /gene="hisD"
                     /locus_tag="PKX01_12590"
     CDS             complement(2446..3762)
                     /gene="hisD"
                     /locus_tag="PKX01_12590"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMZ77012.1"
                     /translation="MSEPIRRLASSDAGFAATLDALLAFEGSTDAAIEAAVADILAAV
                     RRDGDAAVVEFTRRFDHVDAADMAALELPRAELAAALAGLPAEQRTALEAAAERVRRY
                     HLRQVQESWSYVEDTPDMRGTRLGQQVTPLDRVGLYVPGGRAAYPSSVLMNAIPAKVA
                     GVGELIMVVPTPRGEKNPLVLAAAAITGVDRVFTIGGAQAVAALAYGTGSIPQVDKIV
                     GPGNAYVASAKRRVFGTVGIDMVAGPSEVLVISDGSGDPDWVAMDLFAQAEHDELAQS
                     ILLCTDADFIARVAASIVRLLPDMPRRDTIATSLKNRGALIHVRDLAEACDIANRIAP
                     EHLELSLAEPGAWVERIRHAGAIFIGHHSVEALGDYCAGPNHVLPTMRSARFSSPLGV
                     YDFQKRTSLIEVSEAGAQTLGRIASVLARGEGLQAHARSAEMRLKD"
     gene            complement(3759..4403)
                     /gene="hisG"
                     /locus_tag="PKX01_12595"
     CDS             complement(3759..4403)
                     /gene="hisG"
                     /locus_tag="PKX01_12595"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMZ77013.1"
                     /translation="MNGITLALSKGRIFDETLPLLAAAGIVPTDNPESSRKLIIGTNR
                     PDVRLVIVRATDTPTYVQYGAADLGIAGKDVLIEHGGVGLYQPLDLNIAKCRLCVATP
                     KDFDYAAAVKRGARIRVATKYVKTAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGGTLRANNLVEVEDIMPISSRLVVNQASLKLKRELIQPVLEAFAGAVKP"
     gene            complement(4480..5730)
                     /gene="murA"
                     /locus_tag="PKX01_12600"
     CDS             complement(4480..5730)
                     /gene="murA"
                     /locus_tag="PKX01_12600"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMZ77014.1"
                     /translation="MDKLLIEGGARLSGEIAISGAKNAALPILCAALLTKEPVTFTHV
                     PRLNDIGTLLRLLEQMGVKVSRDGDTVTLDAGGVNNPVASYEMVKTMRASILVLGPLL
                     ARFGEARVSLPGGCAIGARPVDQHIKGLQAMGADVKVEHGYVNARSPRLKGARIFTDM
                     VTVTGTENLLMAACLADGETVIENAAREPEVVDLANCLVAMGARISGAGGDVIRIQGV
                     PALHGATHRVMPDRIETGTYLCAAAVTGGEVRLTHTSTSYLDAVVDKLMDAGCEITAE
                     RDAIRLKAPARLTAVSLRTAPYPAFPTDMQAQFMALNTVADGTAVIRETIFENRFMHA
                     VELQRLGADIRIDGNTAVVKGVAGLQGATVMATDLRASASLVIAGLVAEGETTIERIY
                     HLDRGYERLEAKLAALGARVTRLK"
     gene            complement(5744..5998)
                     /locus_tag="PKX01_12605"
     CDS             complement(5744..5998)
                     /locus_tag="PKX01_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HMZ77015.1"
                     /translation="MFLASEIERLIGAGLDCEFLQVEGDDGTHFSGIVVSAAFEGKPK
                     VRQHQAVYATLGKLMGNEIHALQLTTYTPAQWADFQKELG"
     gene            complement(6008..6763)
                     /locus_tag="PKX01_12610"
     CDS             complement(6008..6763)
                     /locus_tag="PKX01_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77016.1"
                     /translation="MEGFKTLFYKECLRFWKVGFQTVLSPVLTAVLYLLIFGHVLEDH
                     VQPYPGVSYTAFLVPGLVMMTVLQNAFANSSSSLIQSKITGNIVFVLLPPISYREFYA
                     AYVLAAILRGALCGLGVLLVSLPFAMPGVAAPGWIVAFTLIGGAFLATLGVIAGIWAD
                     KFDQLAAFQNFLIMPLTMLSGVFYSIHSLPGFWQGVSHFNPFFFMIDGFRYGFFGVSD
                     VSPWTSVGVVSVCFIVLAVATLQMLARGYKLRA"
     gene            complement(6767..7669)
                     /locus_tag="PKX01_12615"
     CDS             complement(6767..7669)
                     /locus_tag="PKX01_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77017.1"
                     /translation="MIPAIRAVGVTKRFRALQALAGVDLEVAQGEFFGLLGPNGAGKT
                     TLISALAGLARADAGTLEVMGHDVVRDYRAARLALGVVPQELVFDPFFSVREMLRIQS
                     GYFGLKRNDDWIDEILHHLDLTPKAHANMRMLSGGMKRRALVAQALVHRPPVIVLDEP
                     TAGVDVELRQGLWQFIRKLNRDGHTVVLTTHYLEEAETLCGRIAMLKAGRIVALDTTA
                     NLLARFANHTLQVRLAAPVSLPPALAAAVVRQDGLDLVLGFERFDDVGALLDALRGAG
                     AQIADMVLGKPDLEQVFVGVMKES"
     gene            complement(7689..7973)
                     /locus_tag="PKX01_12620"
     CDS             complement(7689..7973)
                     /locus_tag="PKX01_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ77018.1"
                     /translation="MIVQDGDCLRLSGALTLTTVSGLLEAGRAAVAAGDCVADLSAVT
                     RVDSAALALLFAWLRAARSAGHRLSLVNAPTALVSLASLYDVDGLLPPAA"
     gene            complement(7970..8599)
                     /locus_tag="PKX01_12625"
     CDS             complement(7970..8599)
                     /locus_tag="PKX01_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77019.1"
                     /translation="MIKKTASFLAALTLSLGFQAAAFANAVAPDALVKGVTDDVIAIV
                     RQDKAIQSGDTRRAVELVETKVLPYFDFAHMTRLAVGKDWKSASADQKSALTDEFKTL
                     LVRTYSNALTQYRNQQIDFKPLKAKPEDTDVVVRTEVRQPGAKPVQIDYALDKQGDAW
                     KVYDVMVAGVSLVTNYRDEFGQQIRSGGVDGLIKSLKDKNKQLEAAPKK"
     gene            complement(8596..9408)
                     /locus_tag="PKX01_12630"
     CDS             complement(8596..9408)
                     /locus_tag="PKX01_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HMZ77020.1"
                     /translation="MKKNSLNFLRVPARIATTLLLAGGLGACASSGGNPQDPWESMNR
                     ATFRFNDVADRAVMRPVAEAYDTVAPLPIKTGVSNVFGNVADLWIGVNNLLQGKVLDG
                     LSDGGRFLINTTVGMLGVFDIATELGLDKHDEDFGQTLGAWGVGSGPYLVLPFLGPST
                     LRDGTSLLLVDLNMDPVWQIDDMGQRNRTTALRLVAKRAQLLGADTAAEQAALDKYGY
                     MRSFYLQYRQNQVYDGSPPRVKDPDDDAALDVPAAAVPPLAAAPSEQRENQE"
     gene            complement(9458..9934)
                     /gene="mlaD"
                     /locus_tag="PKX01_12635"
     CDS             complement(9458..9934)
                     /gene="mlaD"
                     /locus_tag="PKX01_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HMZ77021.1"
                     /translation="MSRTTLDLWVGVFVAMGIAALLFLALKVGSISGSDVQSPYTLSA
                     RFDNIGGLKVRAPVKSAGVLVGRVAEIRYDNERFQAEVVLSVDGRYRFPTDTFANILT
                     SGLLGEQYIGLDPGGEEKTLAPGEVIKKTQSAVVLEKLISQFMFNKAADGDQAPAK"
     gene            complement(9937..10716)
                     /gene="mlaE"
                     /locus_tag="PKX01_12640"
     CDS             complement(9937..10716)
                     /gene="mlaE"
                     /locus_tag="PKX01_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HMZ77022.1"
                     /translation="MLDILRRLGAAAIDALWQLGFIARFFWLILVYSGQSFLRLNLTL
                     RELYFSGVLSLLIILVSGLFVGLVLGLQGYETLQRYGSADALGTLVALSLVRELGPVV
                     AALLFASRAGSAVTAEIGLMKVTEQLKAMDMMAVSPIARVVAPRFWGGVISMPLLAAL
                     FSAMGIFGGWLIGVIFIGVDDGAFWSQMQGAVDFRYDVLNGVIKSAVFGVAVALIAVF
                     EGYDCVPTAEGVSRAITRTVVSSALAILALDFVMTSFMFRG"
     gene            complement(10716..11519)
                     /locus_tag="PKX01_12645"
     CDS             complement(10716..11519)
                     /locus_tag="PKX01_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77023.1"
                     /translation="MSEPLVRLEHVRFAYGERAILRDISLSIPRGQVVAIMGGSGCGK
                     TTLLRLIGGQERAQSGSVRVDGQEVGALGREALYALRRRIGLLFQFGALFTDMTVFDN
                     VAFPLREHTDLPPELIRDLVMMKLHAVGLRGAADLMPGELSGGMARRVALARTIALDP
                     TLLMYDEPFTGLDPISLGVIGQLIRRLNDALGASSVIVTHDVQESLQIVDYIYFISEG
                     RVVAEGTPEAIRNTTDPFVHQFIHAEADGPVPFHYPAGDYATALTGGPR"
     gene            11699..>13713
                     /gene="leuS"
                     /locus_tag="PKX01_12650"
     CDS             11699..>13713
                     /gene="leuS"
                     /locus_tag="PKX01_12650"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMZ77024.1"
                     /translation="MQDKYLPADIERAAQQHWDSTQAFRVTADASRPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHKMKGYNVLQPMGWDAFGLPAENAAIKNRVPPAKWTY
                     DNIDYMKGQLKSLGFAIDWSRELATCRPEYYKWNQWLFLRMLEKGIAERKTQVVNWDP
                     VDQTVLANEQVIDGKGWRTGAVVEKREIPGYYLKITDYADELLSDLDSLTGWPERVKL
                     MQANWIGKSIGVRFAFGHDIAGDDGKLISDGKLWVFTTRADTIMGVTFCAVAAEHPLA
                     VHAAQDNPALAAFIEKCKHGSVMEADMATMEKEGLPTGLFVTHPLTGEQVEVWVGNYV
                     LMSYGDGAVMGVPAHDERDFGFAKKYGIAIKQVIANGDKAFGLDGWQEWYGAKDGVCV
                     NSGKYDGLGYEAAVDAVATDLAALGVGEKKVQWRLRDWGISRQRYWGCPIPIIHCDAC
                     GSVPVPDDQLPVVLPEDCVPDGSGNPLHKRDDFIHTTCPCCGKPARRETDTMDTFVDS
                     SWYYARFATGNGANTMVDAETDYWMTVDQYIGGIEHAILHLLYSRFWTKVMRDLGLVS
                     YREPFAHLLTQGMVLNHAFFHKPDGGGKNYYWESEIDLLRDSKGQVMGAKLKTDGTVL
                     EHELTTMSKSKNNGVDPQALIEQYGADTARFFMMFAAPPEQTLEWSDA"
BASE COUNT         2362 a         4596 c         4615 g         2140 t
ORIGIN      
        1 actggacggc aaagatatta gcctgcgcta ccgccccggt aaaggggatg ccgtactcgg
       61 tggtggcggc gatcaccgcc gggtcggccg gatcgacgta atagctgtgc acgtaataaa
      121 aacggctgcc gtccggaatg cctgcccata ggggatgggc ctgcttctgc cagacctcgt
      181 tccatcccat gtggggcacc ttgaggcggc caccgtcggg cgcggtcatc cgcgcatcgg
      241 acggaaagcg ccgcacgtga ccacgaaaca ggcccagccc gggcacgtca ccctcgtcgc
      301 tgtgatcgaa gagcatctgc tggccgatgc agatgcccag aaagggcttg ctgcgcgccg
      361 cctccagcac cgccgggcgc aggccgcggg cgtccagttc acgcatgcag tcgggcatcg
      421 caccctggcc ggggaagacc acccgctccg ccgccagcac cgcggccgga tcagcggtca
      481 ccaccaccgt tgcaccggtg gccacatgct cgatggcctt ggccaccgag cgcaggttgc
      541 ccatgccgta atcgatgatc gccacgctgc tcatgtcaca gggctccctt ggtggagggc
      601 accacgccgg cctggcgtgg atcgggcgtc accgccatgc gcagggcgcg cccgaaggct
      661 ttgaagacgg tttcgcattg atggtgggca ttgcggccct tgaggttgtc gacatgcagg
      721 ctcacgcccg catggttgac gaagccctgg aaaaattcgt agacgagctg ggtatccagc
      781 gcgccaatgg ccgccgccgt gaagggcacg tcgaattcca gccccggccg gccggacagg
      841 tccaccacca cccgggacag ggcctcatcg aggggcacat aggcatgacc gtagcgggtc
      901 aggcccttct tgtcgccgat ggcccgggcg aaggcctgac cgagggcgat gcccacgtcc
      961 tccaccgtgt ggtggccgtc gatgtgcagg tcgcccttcg ccgcgatctc cagatcaaag
     1021 cagccgtggc gggcaatctg gtcgagcatg tggtcgaaga agccgatacc ggtatcgagc
     1081 cggctctggc cggagccgtc cagatccaga cggacgcgga tctgcgtttc gagggtgtcg
     1141 cgggagattt cagcctggcg catgcgctgt gttccagaaa atcgttgaaa gccggtgaat
     1201 acgaacggtc atgatagcat ttcccatttt caccgtccct ggcggcgccc ccggacgccg
     1261 cgccgatctg tcatgagccg cttctggagc cccgtcgtcc acgacctgac gccctacgtc
     1321 ccgggcgagc agcccaagct cgccaacctg gtcaagctca acaccaacga aaatccctat
     1381 ccgcccagcc cgcgggtggt ggacgccatc cgcgccgagc tgggcgagga cggcgaccac
     1441 ctcaagctct acccggatcc caccggaagc gccctgaagg ccgccgtgag ccgccaattc
     1501 ggcgtgccgg cggagtgggt gttcgtcggc aacagctcgg acgaagtgct cgcccatacc
     1561 ttccagggcc tgctcaagca cgacgcgccg ctgctcttcc cggacatcac gtatagcttc
     1621 tatccggtgt attgcggcct ctacggcatc cccttcgaga ccgtgccgct ggccgaggac
     1681 ttctcgatcc gcgtggacga ttacctcgtc cgcggacccg ccgcggtcgg cggcgtgatc
     1741 ttccccaatc ccaacgcgcc gacggggcgc ctgctgcccg tcgccgagat cgcccgcctc
     1801 gccgccggcc tgcccgacag cccggtggtg gtggacgagg cctacgtgga tttcggcggg
     1861 cacgacgcga ttcccagcgc ggtggcgctg gtggccgagt accccaatat cctggtcacc
     1921 cagactctct ccaagtcccg ttccctggcc gggctgcggg tcggcttcgc gatcggccag
     1981 ccggaactgg tggaagccct cacgcgggtc aaggacagct tcaactccta cccgctggac
     2041 cgcctcgccc tggtcggcgc ctgcgcagcg ctggacgaca cggagtggtt ccattgcacc
     2101 cgccatcagg tcatcgccag ccgcgaggcc ctcagcgcgt ccctgaccaa cctgggcttc
     2161 gaggtcctgc cctctgccgc caacttcgtc ttcgcccgcc acccggccca cgacgcggcc
     2221 ctgctgtccg ccgccctgcg cgcgcgcagc atcatcgtgc gccacttccg tgccccgcgc
     2281 atcgaccagt tcctgcgcat cacggtgggc accgatgccc agtgcgcgca gctggtgacg
     2341 gccctgggcg agatcctcgg ctgaccgccc gccggcgaac accgcaacga aaaaccgcgc
     2401 ccgaaggcgc ggttttttat gggcagacgc aaatggacgg ccggctcagt ccttcagccg
     2461 catctccgcc gaacgggcat gggcctggag gccttcgccg cgggcgagca ccgaggcgat
     2521 gcggcccagg gtctgggcgc cggcctcgga gacttcgatg aggctggtgc gcttctgaaa
     2581 gtcgtacacc ccgagcgggc tcgagaagcg cgcgctgcgc atggtgggca gcacgtggtt
     2641 ggggccggca cagtagtcgc ccagggcctc gacggagtgg tggccgatga agatcgcgcc
     2701 cgcgtggcgg atgcgctcga cccacgctcc cggctcggcg agggacagct ccaggtgctc
     2761 cggcgcaatg cggttggcga tgtcgcaggc ctcggccaga tcgcgcacat ggatcagcgc
     2821 gccgcggttc ttcagggagg tggcgatggt gtcgcgacgg ggcatgtcgg gcagcaggcg
     2881 gacgatgctc gccgccacgc gggcgatgaa gtccgcgtcg gtgcacagca ggatggactg
     2941 ggccagctcg tcatgctcgg cctgggcgaa gagatccatc gccacccagt ccggatcgcc
     3001 ggagccgtcg gagatgacca gcacttcgga cggtccggcc accatatcga tgcccacggt
     3061 gccaaagacg cggcgcttgg cgctggccac gtaggcgttg ccggggccga cgatcttgtc
     3121 cacctggggg atgctcccgg tgccataggc cagggcggcc accgcctggg cgccgccgat
     3181 ggtgaacacc cgatccacgc cggtgatggc agcagcagcg aggaccagcg ggttcttctc
     3241 gccccgcggt gtgggcacca ccatgatcag ctcgcccacc ccggccacct tggcggggat
     3301 ggcgttcatc agcacggagc tgggataggc ggcacgcccg cccggcacgt agagtccgac
     3361 ccggtccagc ggcgtgacct gctggcccag gcgggtgccg cgcatgtcgg gcgtgtcctc
     3421 gacgtagctc caggattcct gcacctgacg caggtggtag cggcgcaccc gctcggcagc
     3481 agcttcgagg gccgtgcgct gctcggcagg caatccggcg agggcggccg ccagctcggc
     3541 gcgggggagt tccagcgcgg ccatgtcggc ggcgtccacg tggtcgaagc gacgggtgaa
     3601 ttccaccacc gcggcatcgc catcccgtcg caccgcggcc agtatgtcgg ccacggccgc
     3661 ctcgatggcg gcgtcggtgc tgccctcgaa ggccagcagg gcgtccaggg tggcggcgaa
     3721 gccggcgtcg gaagacgcca ggcggcggat cggttcgctc atggcttgac ggctccggca
     3781 aaggcctcca gcaccggctg gatgagttcg cgtttgagct tgagggaggc ctgattgacg
     3841 acgaggcggg agctgatggg catgatgtcc tccacctcca ccagattgtt ggcgcgcagg
     3901 gtgccgccgg tggataccag atcaacgatg gcgtccgcca ggcccaccag cggcgccagt
     3961 tccatggagc cgtagagctt gatcagatcc acgtgcacgc ccttggcggc aaagtgctcg
     4021 cgggcggtct tgacgtactt ggtggccacg cggatgcgcg cgccgcgctt gacggcggcg
     4081 gcgtagtcga aatccttggg tgtcgccacg cacaggcggc acttggcgat gttcaggtcg
     4141 aggggctggt acaggccaac gccgccgtgt tcgatgagca cgtccttgcc ggcgatgccc
     4201 aggtccgccg cgccgtattg cacataggtg ggcgtgtcgg tggcgcggac gatgacgagg
     4261 cgcacgtccg gccggttggt gccgatgatg agcttgcgcg acgactcagg gttgtcggtg
     4321 ggcacgatgc cggccgcggc gagcagcggc agggtttcgt cgaagatgcg gcccttggag
     4381 agagcgagag tgatgccgtt cacggtcgtg ttctgcgtgg caaaggagca attctagccg
     4441 agccgccaca gcggcccggc gatttccgcg gaatgcggcc tacttgagtc gggtgacgcg
     4501 ggcgcccagg gcggcaagct tggcttcgag ccgctcgtag ccccggtcca ggtggtagat
     4561 gcgctcaatg gtggtctcgc cttccgccac cagaccggcg atcaccaggc tggccgatgc
     4621 gcgcaggtcg gtggccatca ccgtggcgcc ctgcagaccg gccacgccct tcaccactgc
     4681 cgtgttgccg tcgatgcgga tgtcggcgcc gaggcgctgg agctccaccg cgtgcatgaa
     4741 gcggttctcg aagatggtct cgcggatcac cgcagtgccg tcggccaccg tgttgagggc
     4801 catgaactgg gcctgcatgt cggtgggaaa agccggatag ggcgcggtac gcaggctcac
     4861 cgcggtgagg cgggccggcg ccttgaggcg gatcgcgtcg cgctcggcgg tgatctcgca
     4921 accggcgtcc atcagcttgt ccaccaccgc gtccaggtag ctggtggagg tatgggtcag
     4981 gcgcacctcg ccgccggtga cggccgcggc gcacaggtag gtgccggtct cgatgcggtc
     5041 cggcatcacc cgatgggtgg cgccgtggag ggccggcacg ccctggatgc ggatcacgtc
     5101 accgcctgcg ccggagatgc gcgcgcccat ggccaccagg cagttggcca ggtccaccac
     5161 ttcgggctcg cgggcggcgt tctcgatgac ggtctcgccg tcggccagac aggcggccat
     5221 cagcaggttt tcggtgccgg tgacggtgac catgtcggtg aagatccgcg cccctttcag
     5281 gcggggcgag cgggcattga cgtagccgtg ctccaccttg acgtccgccc ccatggcctg
     5341 caggcccttg atgtgctggt ccaccggccg cgcgccgatg gcgcagccgc cgggcaggct
     5401 cacccgcgcc tcgccaaagc gcgccagcag cggccccagc acgaggatgg aggcacgcat
     5461 ggtcttgacc atctcgtagg acgccaccgg gttgttcacc ccgccggcgt cgagggtcac
     5521 cgtgtcgcca tcgcggctga ccttcacgcc catctgttcg agcaggcgca gcagggtgcc
     5581 gatgtcgttg aggcgcggta cgtgggtgaa ggtcaccggc tccttggtca gcagggccgc
     5641 acacaggata ggcagggccg cgttcttggc gccggaaatg gcgatctcgc ccgacaggcg
     5701 ggcgccgcct tcgatcaaca gtttatccat gcatcagggg cgttcagccc agttccttct
     5761 gaaagtcggc ccattgggcg ggcgtgtagg tggtgagctg gagggcgtgg atctcgttgc
     5821 ccatcagctt gccgagggtg gcatagacgg cctggtgctg gcgcaccttg ggtttgccct
     5881 cgaaggcggc actcaccacg atgccagaga agtgggtgcc gtcgtcccct tccacctgca
     5941 ggaattcgca gtccagtccg gccccgatga gccgttcgat ttcacttgcc agaaacatgt
     6001 tggatcctca ggcccgcagc ttgtatcccc tggccagcat ctgcagggtg gccaccgcga
     6061 gcacgatgaa acagaccgaa accactccga cgctcgtcca cggcgagacg tcggagaccc
     6121 cgaaaaatcc gtaacggaac ccgtcgatca tgaagaaaaa cgggttgaaa tgcgagaccc
     6181 cctgccagaa acccggcagg gagtggatgg agtagaacac gccggacagc atggtgaggg
     6241 gcatgatcag gaagttctgg aaggcggcga gctggtcgaa cttgtccgcc cagattccgg
     6301 cgatcacccc cagcgtggcc aggaaggcgc cgccgatcag cgtgaaggcc acaatccacc
     6361 cgggtgccgc cacgccgggc atcgcaaagg gcagcgacac gagcagcacg cccagcccgc
     6421 acagggcgcc gcgcaggatg gcggccagca cgtaggcggc gtagaactcc cgataggaga
     6481 tgggcggcag cagcacgaag acgatgttgc ccgtgatctt gctctgaatg agggaggacg
     6541 agctgttggc gaaggcgttc tgcagcaccg tcatcatcac caggccgggc accaggaagg
     6601 ctgtgtagct gacgccggga tagggctgga cgtggtcttc cagcacgtgc ccgaagatca
     6661 gcaggtacag cacggcggtc agcaccggcg acagcacggt ctggaaaccc accttccaga
     6721 agcgcaggca ttccttatag aacagggtct tgaagccttc catggctcag ctttccttca
     6781 tcacgccgac gaagacctgc tccaggtcgg gcttgccgag gaccatgtcg gcgatctgtg
     6841 cacccgcgcc gcgcagggcg tcgaggagcg cacccacatc gtcgaagcgc tcgaagccga
     6901 gcaccagatc gagaccgtcc tggcgcacca ccgccgcggc gagggccggg ggcagactca
     6961 ccggcgcggc caggcgcacc tgcagggtgt ggttcgcaaa tcgcgccagg agattggcgg
     7021 tggtatccag ggcgacgatg cggccggcct tgagcatggc gatacgcccg cacagggtct
     7081 ccgcctcttc cagatagtgg gtggtgagca ccaccgtgtg gccgtcccgg ttgagcttgc
     7141 ggatgaactg ccacagtccc tggcgcagct ccacgtccac gccggcggtg ggttcgtcca
     7201 gcacgatcac cggcggacga tgcaccaggg cctgggccac cagcgcccgc cgcttcatgc
     7261 cgccggagag catgcgcatg ttggcgtggg ccttgggcgt caggtccagg tgatgcagga
     7321 tctcgtcgat ccagtcgtcg ttgcgcttga gtccgaagta gccggactgg atgcgcagca
     7381 tctcgcgcac cgagaagaac ggatcgaaga ccagttcctg gggcaccacc cccagggcca
     7441 gacgggcggc acggtagtcg cggaccacgt cgtgacccat cacctccagc gtgccggcgt
     7501 cggcgcgggc aagcccggcg agggcggaga tgagggtggt cttgccggcg ccgttggggc
     7561 cgagcaaacc gaagaactcg ccctgggcga cctccaggtc caccccggcc agggcctgca
     7621 gggcacggaa acgcttggtg acgccgacgg cgcggatcgc aggaatcatg ggttcggagc
     7681 gccccggctc aggccgccgg gggcaggagg ccatccacgt cgtagaggga cgcgaggctg
     7741 acgagggccg tcggggcgtt gacgaggctg aggcgatggc cggcgctgcg cgcagcccgc
     7801 agccaggcga agagcagcgc cagggctgcg gaatccacgc gcgtcaccgc ggagaggtca
     7861 gcgacgcaat ctcccgccgc gaccgccgcc cgacctgcct cgagcaggcc gctgacggtg
     7921 gtgagggtga gcgcgccgga gaggcgcaga cagtcgccgt cctggacgat catttcttgg
     7981 gcgccgcctc aagctgcttg ttcttgtcct tgagggactt gatgaggccg tccacgccgc
     8041 cgctgcggat ctgctggccg aactcgtccc ggtagttggt gaccagactc acgcccgcca
     8101 ccatcacgtc gtagaccttc caggcatcgc cctgcttgtc cagggcgtag tcgatctgca
     8161 ccggcttggc gcccggctga cggacttcgg tgcggaccac cacgtcggtg tcttccggct
     8221 tggccttgag gggcttgaag tcgatctgct ggttgcgata ctgggtcagg gcattggagt
     8281 aggtgcgcac cagcagggtc ttgaactcgt cggtgagggc gctcttctgg tcggcgctgg
     8341 cgctcttcca gtccttgccc acggccaggc gggtcatgtg ggcgaagtca aaatagggca
     8401 gcaccttggt ttcgaccagc tcgacggcgc ggcgggtgtc gccggactgg atcgccttgt
     8461 cctggcggac gatggcgatg acgtcgtcgg tgacgccctt gaccagggcg tcgggcgcca
     8521 ccgcgttggc aaaggcggcc gcctggaaac ccaggctgag ggtcagggcg gcgaggaagg
     8581 aggcggtctt cttgatcatt cctggttctc tctctgttcg gatggggcgg ctgccagggg
     8641 cgggacggca gcggccggca catcgagcgc cgcgtcgtcg tccgggtcct tcacccgcgg
     8701 cggggagccg tcgtacacct ggttctgccg gtattgcagg taaaagctgc gcatgtagcc
     8761 atacttgtcg agggcggcct gctcggcggc cgtgtcggcg cccagcagct gcgcccgctt
     8821 ggcgaccagg cgcagggccg tggtgcggtt gcgctggccc atgtcgtcga tctgccacac
     8881 cgggtccatg ttcagatcca ccagcagcag actcgttccg tcccgcaggg tgctcggccc
     8941 gaggaagggc agcaccagat acgggccgga gccgacgccc caggcgccca gagtctggcc
     9001 gaagtcctcg tcatgcttgt cgagccccag ctcggtggcg atgtcgaaaa caccgagcat
     9061 gccgacggtg gtgttgatca ggaaacgccc gccgtccgac agaccgtcga ggaccttgcc
     9121 ctggagcagg ttattcacgc cgatccacag gtccgccacg ttgccgaaga cgttgctgac
     9181 gccggtcttg atgggcagcg gcgccaccgt gtcgtaggct tcggcgaccg gacgcatcac
     9241 ggcccggtcg gccacgtcgt tgaagcggaa ggtggcccgg ttcatggact cccagggatc
     9301 ctggggattg ccgccggacg aggcgcatgc acccagacca ccggccagca gcagggtcgt
     9361 cgcgatgcgg gccgggaccc gcaggaaatt taggctgttc ttcttcatcg tcgttccgca
     9421 gccggtcggg gccgggtgcg ctctccgtgg ccggcggcta cttggccggc gcctgatcgc
     9481 cgtctgccgc cttgttgaac atgaactggc tgatgagctt ttcgagtaca acggcggact
     9541 gggtcttctt gatgacttcg cccggcgcca gggtcttctc ctcgccaccg ggatcgaggc
     9601 cgatgtactg ctcgccgagc aggcccgagg tgaggatgtt ggcgaaggtg tccgtcggaa
     9661 acctgtagcg tccgtccacc gaaaggacca cctccgcctg gaagcgttca ttgtcgtagc
     9721 ggatctcggc gacgcggccc accagcacgc cggcgctctt gaccggcgcg cgcaccttga
     9781 ggccgccgat gttgtcgaag cgggccgaca gggtgtaggg cgactgcacg tcgctgccgg
     9841 aaatgctgcc caccttcagc gccagaaaga gcagcgcggc gatgcccatc gccacgaaaa
     9901 cccccaccca cagatcgagc gtcgtgcggc tcatcgtcaa cctcggaaca tgaaagacgt
     9961 catcacaaaa tccagcgcca ggatcgccag cgcggaactc accacggtgc gggtgatggc
    10021 ccgcgacacg ccctcggcgg tgggcacgca atcgtagccc tcgaacacgg cgatcagcgc
    10081 caccgccacg ccgaacacgg cgctcttgat gacgccgttg agcacgtcgt agcggaaatc
    10141 caccgcgccc tgcatctgcg accagaacgc gccgtcatcg acaccgatga agatcacccc
    10201 gatgagccag ccgccgaaga tgcccatcgc cgagaacagg gccgcgagca ggggcatgga
    10261 gatcacgccc ccccagaagc gcggcgccac cacgcgggcg atggggctca ccgccatcat
    10321 gtccatggcc ttgagctgct cggtgacctt catcaggccg atctccgccg tcaccgccga
    10381 accggcgcgg ctggcaaaga gcagcgccgc caccaccggc cccagctccc gcaccaggga
    10441 cagggccacc agggtgccga gggcgtcggc cgaaccgtaa cgctggagcg tctcgtagcc
    10501 ctgcaggccg agcaccaggc cgacgaagag accggagacg aggatgatca gcagggacag
    10561 cacgccgctg aagtagagct cccgcagggt caggttcagg cgcaggaagg actggccgga
    10621 atacaccagg atcagccaga agaagcgggc gatgaagccc agctgccaga gggcgtcgat
    10681 ggccgcggcc ccgagccggc gcaggatgtc cagcatcagc gcggccctcc ggtgagggcc
    10741 gtggcgtaat cgccggccgg atagtggaag ggcaccggcc cgtcggcttc cgcgtggatg
    10801 aactgatgca cgaaggggtc ggtcgtgttg cggatcgcct cgggcgtgcc ttcggccacc
    10861 acccggccct ccgagatgaa atagatgtaa tccacgatct gcagggattc ctgcacgtcg
    10921 tgggtgacga tgacggagct cgcccccagc gcatcgttga ggcgccggat cagctggccg
    10981 atcacgccca gggaaatcgg atcgaggccg gtgaagggct cgtcgtacat gagcaacgta
    11041 gggtcgaggg cgatcgtgcg ggccagcgcc acgcgccgcg ccatgccgcc ggacagctcg
    11101 cccggcatca gatcggccgc cccgcgcagc cccaccgcgt ggagcttcat catcaccaga
    11161 tcgcggatca gctcgggtgg caggtcagtg tgctcgcgca gcggaaaggc cacgttgtcg
    11221 aacaccgtca tgtcggtgaa cagggccccg aactggaaca gcaggccgat gcgccggcgc
    11281 agcgcgtaaa gcgcctcgcg accgagggcg cccacctcct gaccgtccac ccggaccgag
    11341 ccggactgcg cccgctcctg gccgccgatc aggcgcagca gcgtggtctt gccacagccg
    11401 ctaccgccca tgatggccac cacctgaccc cgcggaatcg acagggaaat gtcgcgcagg
    11461 atggcgcgtt cgccgtaggc gaagcgcacg tgctcaagac ggacaagggg ctcggacatg
    11521 gaaaatgctg caatgcggga gcgcggattg tagcagaggc gtgctgccgt ggcatgcccg
    11581 ggacaaccct gacaggccga aataccccgt cacatcaatg tcttgatgca gcgggcgcgc
    11641 ctgtcgcgcg agcagtctat aatgtgcggt ttggttccac ttgcccgcct ttccgcccat
    11701 gcaggacaaa taccttcccg ccgacatcga gcgcgccgcc caacagcatt gggacagcac
    11761 ccaggccttc cgcgtcaccg ccgacgcctc gcgccccaag tattactgcc tgtccatgtt
    11821 cccgtatccg tcgggcaagc tgcacatggg ccacgtgcgc aactacacca tcggcgacgt
    11881 gctctcccgc tttcacaaga tgaagggcta caacgtcctg cagcccatgg gctgggacgc
    11941 cttcggcctg cccgccgaga acgcggccat caagaaccgc gtcccgccgg ccaagtggac
    12001 ctacgacaac atcgactaca tgaagggcca gctcaagtcc ctgggcttcg ccattgactg
    12061 gagccgcgaa ctggccacct gccgccccga gtattacaag tggaaccagt ggctgttcct
    12121 gcgcatgctc gaaaaaggca tcgccgagcg caagacccag gtggtgaact gggacccggt
    12181 ggaccagacc gtgctggcca acgagcaggt catcgacggc aagggctggc gcaccggcgc
    12241 ggtggtggaa aagcgcgaga tccccggcta ctacctgaag atcaccgact acgccgacga
    12301 gctgctgtcc gacctggaca gcctcaccgg ctggcccgag cgggtcaagc tgatgcaggc
    12361 caactggatc ggcaagagca tcggcgtgcg cttcgccttc ggccacgaca tcgccggcga
    12421 tgacggcaag ctcatcagcg acggcaagct gtgggtgttc accacccgcg ccgacaccat
    12481 catgggcgtc accttctgcg ccgtggccgc cgagcatccc ctggccgtcc acgccgccca
    12541 ggacaacccc gccctcgccg ccttcatcga gaagtgcaag cacggctccg tcatggaagc
    12601 cgacatggcc accatggaga aggaaggtct gcccaccgga ttgttcgtca cccacccgct
    12661 gaccggcgaa caggtggaag tctgggtcgg caactacgtg ctgatgagct acggcgacgg
    12721 cgccgtgatg ggtgttccag cccacgacga gcgggatttc gggtttgcca agaaatacgg
    12781 catcgcgatc aagcaggtca tcgccaacgg cgacaaggcc ttcggcctgg acggctggca
    12841 ggagtggtac ggcgccaagg acggcgtctg cgtaaactcc ggcaagtacg acggtctcgg
    12901 ctacgaagcc gccgtcgatg ccgtcgccac cgatctcgcc gccctgggcg tcggcgagaa
    12961 gaaagtccag tggcgtctgc gtgactgggg catctcccgt cagcgctact ggggctgccc
    13021 gattcccatc atccattgcg atgcgtgcgg atccgtcccc gtgcccgacg accagctccc
    13081 cgtggtgctc cccgaagact gcgtgcccga cgggtccggc aacccgcttc acaagcgcga
    13141 cgacttcatc cacacgacct gtccctgctg cggcaagccg gccaggcgcg aaaccgacac
    13201 catggacacc ttcgtggatt cgtcctggta ctacgcccgt ttcgccacgg gcaacggcgc
    13261 gaataccatg gtggatgccg agaccgacta ctggatgacc gtggaccagt acatcggcgg
    13321 catcgaacac gccatcctgc acctgctcta ttcccgcttc tggaccaagg tcatgcgcga
    13381 cctgggcctg gtgagctacc gcgaaccctt cgcccacctg ctcacccagg gcatggtgct
    13441 caaccacgcc ttcttccaca aacccgatgg cggcggcaag aactactact gggaaagcga
    13501 gatcgacctg ctgcgcgact ccaagggcca ggtcatgggc gccaagctca agaccgacgg
    13561 cacggtgctg gagcatgagc tcaccaccat gtccaagtcc aagaacaacg gcgtggatcc
    13621 gcaggccctc atcgagcagt acggcgccga caccgcccgc ttcttcatga tgttcgccgc
    13681 gccgcccgag cagaccctcg aatggtccga cgc
//
LOCUS       DAOQPA010000094        13631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2328_length_13631_cov_17.903064, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000094 DAOQPA010000000
VERSION     DAOQPA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_13631_cov_17.903064"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKX01_12655"
     CDS             <1..951
                     /locus_tag="PKX01_12655"
                     /inference="COORDINATES: protein motif:HMM:NF021201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HMZ77025.1"
                     /translation="DDDPLVRRFASDGLWEIWGRAGTPEQNLKLEQAGRLTDPDAERA
                     AWAHARGHVFTHPALAAAATVLDALPPARALARHALLRALDTWQADGRSGTWRDFSHF
                     ARGSTKLIGDSERAWLAAHLTLADFRLDDHTPLLLLRTAAELRLGDTHLTLAAIPDFV
                     ALSPDTLRQLTGAASGPAVWFVVENRSSFERVARACGPDEGALWLPGYPPGSWRASVA
                     GLLRHLPAPARIACDPDPDGILIACQAGTLWTAAGLDWTPWRMDAADFDALKTTRPLT
                     ERDRKLLDSLLHDPTLPAPLTHLAKALATRGAKGEQEAYL"
     gene            1052..2152
                     /locus_tag="PKX01_12660"
     CDS             1052..2152
                     /locus_tag="PKX01_12660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ77026.1"
                     /translation="MPTSSAALPAPSQVLQLLAGAEALSRERDLRTLFRLGAEYAMRF
                     LDGRFGLFFQVRFTPPELTVGLLGCCVSDGADTHWDDVAEAQSWLPVDPLLAVCLHAD
                     HGHAIEQSGDIWRVAFAVGALGDARWALEIQTPRKPSKTDGEALSLFLRCFENVHRQW
                     EYANLDTLTRLLNRKTFDDDFDRLIAAAELAEQARQQPQDRRDPGLKITQPCWLAVVD
                     IDHFKHINDTYGHLFGDEVLLRLADLMRQSFRNHDKLFRFGGEEFVIMLRNVSEDHVQ
                     PIFDRFRMAVDRNDFPRVGHVACSIGFTRIDPRFSPAELLGRADEALYFSKEHGRNQV
                     NGFDTLIMSGQLRLSTPAGQAQIQSDVDEIFL"
     gene            2324..3829
                     /gene="lysS"
                     /locus_tag="PKX01_12665"
     CDS             2324..3829
                     /gene="lysS"
                     /locus_tag="PKX01_12665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMZ77027.1"
                     /translation="MSDQTSAPAQDENHLIAERRQKLAEWRATGKAFPNDFARENTAG
                     KLDEVYGEKSAEELEALPVEVKVAGRIMLKRVMGKASFATIQDLSGRIQLYVTRDGVG
                     EDVYAQFKHWDIGDIVGTVGTLFRTKTGELSVKSTELRLLTKSLRPLPDKFHGLADVE
                     TKYRQRYVDLIMSEESRRTFVARSRLVQSIRNYMTGHGFLEVETPMMHPIPGGAAAKP
                     FVTHHNALDMQQFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGLSPRHNPEFTMMEF
                     YEAYADYRSLMDFTEGLLRHAAREALGTEVFQYQGRELDLSRPFHRLTIVEAIRKYHP
                     GFTEAQLADAEWIKTKIVAFGEKVKPGGLGSLQLQLFEACTEADLWEPTFIIDYPVEV
                     SPLARASDTDPEITERFELFIVGREIANGFSELNDPEDQAARFQAQALAKDAGDEEAM
                     YYDADYIRALEYGLPPTGGCGIGIDRLIMLLTDSPAIRDVILFPQMRPEAD"
     gene            complement(3929..6097)
                     /locus_tag="PKX01_12670"
     CDS             complement(3929..6097)
                     /locus_tag="PKX01_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ77028.1"
                     /translation="MKAKSIQGRISMAAGSCLIGSAIALVAFSVISSDATKSVVAERV
                     STLVKNDTAESLKHLAGTQAGRIQAKFDLALDAARTMAHTFVLAKTPGSGVSLGRGQI
                     NAILKNVLENNPEFNGTYSCWEPNALDGQDAAHATGLNGDNAKTGRFTPYWNRGEGGR
                     IAVQPLVEYDTLDKHPNGVLKGGWYIGPRDQHTESVLDPFPYIVQGKQVWLTTLSVPI
                     MVDGKFYGVAGTDYNLNFVQQLAEETDKGLFEGRGEVTIVSNMGLVVADSEHPELIGK
                     RLELNGPANANLMTDIQSGKARGWTDEGSGIMSAVAPIALGRTGKPWAVLVRVPTKVV
                     LAQAQQLDDDIQARGRSSTILLIVAGLAISVGATAILWFAAGSIARPIREAAQFARLI
                     RRGDFSKRFQHHSEDEVGHLAHALDEMSQGLLSKARLAEQISEGDLNVDVELASDDDQ
                     LGRALKRMVDTLNNLVSELQGGANLIASGASQVADLSAVLTDGAGQSAESVTEIGAAI
                     SEMEVQTRNNAQNSGMANTISIRSRDAASLGSQHMDELTAAIQSISDASSRINGIINA
                     INEITNQTNLLALNAAIEAARAGETGRGFAVVADEVRKLASRSAEAAAQASQLISESG
                     ARTSHGIEIARRTADALKLIVTSASEVSELVAQIATASQDQASGISEVSAGLGQIDGV
                     AQRTNGHAVECSEAAAALTKQADHVKHLMGRFRVRSGRPV"
     gene            6632..7171
                     /locus_tag="PKX01_12675"
     CDS             6632..7171
                     /locus_tag="PKX01_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ77029.1"
                     /translation="MSVRDRLLDAGVSLLRDQGASALTQPRIAAAAGVSQSHLTYYFP
                     TRDALLLAIAEHSVDQELQRATADSGGHPAERLAQAARFLPRVRMMLGLVASGDRDPA
                     IRPAFARLATHVREALGQLLAHHGYTVSAPQVVVVHAALVGLAIMNLGRQDATSDADV
                     ELGLRQLLALLPHPERTEP"
     gene            7168..8136
                     /locus_tag="PKX01_12680"
     CDS             7168..8136
                     /locus_tag="PKX01_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ77030.1"
                     /translation="MTSRACLPIALSLAVAACGTPERSDFQGYVEGEYLYLAAPQAGY
                     LKRLDAPRGRRVTDGEPLFEIAADPDDQALAEAQARAGAAREKLENLREPRRTPEIAA
                     LEAALTGAVAARRLAEVQLQRQQALSRQQFVSPAAVDDARARRDQAVAGEDAARQQLA
                     TARIALGRAPEVRGAEADLRAADAQAAQKRWALERKTVAAPTAGEISDTYYRPGEWIP
                     AGAPVTSLLPDTRRRLRFYVPETALAGLQPGQVVEARCDGCAAPIRATIDFIAPQAEY
                     TPPVIYSRGTREKLVFRVEAAPAPDDARRLRPGLPVDVRLAERRDG"
     gene            8129..9055
                     /locus_tag="PKX01_12685"
     CDS             8129..9055
                     /locus_tag="PKX01_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77031.1"
                     /translation="MADWVIDVCGLNKHFGARHVVRDLSLQVRAGEIFGFLGPNGSGK
                     TTSIRMLCGLLTPDSGAGTCLGHDVIRESAAIKRQVGYMTQKFSLWDDLSLAENLDFV
                     ARMFGMDDRRAAVDRTLVELGLEGRRHQLAGTLSGGWKQRLALAACLLHRPRLLLLDE
                     PTAGVDPKARRDFWEEIHRLSAAGITILVSTHYMDEAERCHRLAYIAYGNLLASGTAA
                     EVVAGRGLHTWAVSGAASHGLADTLRRLPGAEQVASFGTALHVSGTDGARLGTALDEL
                     GRRGDLRIEAVQPSLEDAFIHLMQHSQDPLAG"
     gene            9061..10185
                     /locus_tag="PKX01_12690"
     CDS             9061..10185
                     /locus_tag="PKX01_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77032.1"
                     /translation="MGIPDRFSFSRWLGILIKEFIQLKRDRLTFGMIVGIPILQLLLF
                     GFAINSDPRHLPTAVVMADPGPFARAYVAALRNSDYFDVRGSVDAREADRLLERGDVL
                     FVVSFPPGFHRDLVRGRSPTVLVEADATDPMATAGAISVLNKLGAEVFAADLPGLERP
                     PAAAIDLRVQRRYNPEGITAYNIVPGLMGVILTMTMVLMTGLAMTRERERGTFENLLA
                     TPATPLEVMTGKIVPYILIGLIQVSLILAAARWIFDVPMFGNLLVLYAVVLLFICANL
                     TLGITFSSLARNQLQAMQMTFFFFLPSMLLSGFMFPFRGMPEWAQAVGNLLPLTHFLI
                     LVRGILLKGNGVLWEQVWPILAFMGAVLAVGLKTFRRTLD"
     gene            complement(10217..11818)
                     /locus_tag="PKX01_12695"
     CDS             complement(10217..11818)
                     /locus_tag="PKX01_12695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77033.1"
                     /translation="MKKSVLPRVGPVARLSLGLVALTLTLLMAGDWLFGFASSETAMI
                     QEARRREGENLAAQLTVLVEANDELGLGRTLQRILTRNGELRSLAVRRSDGTLMAQRG
                     DHAVHWIAPESGRSTLEHLRVPLNAGRSHWGDVEISFAPTHAPGAGAWLAQPLTRIIL
                     LVSVGGFLVYYAYLRRAMQFLDPSSAVPERVRKAFDTLTDALLVLDADGRIVLANKAF
                     RQLHGESPTRLIGRRIADLDWLDAGMESFEKGRAPWERVLRGDDLVTDVRMSVVRPGG
                     GTLELAVSCSPVRDEYGNTRGCLVSFDDLSELERANQDLRKALSEIEQSRARIKAQNE
                     KLTHLASRDPLTGALNRRAFFEKVAGPFETALRGNGQLCCLMTDIDHFKRFNDIYGHA
                     VGDQVIKVVVRCIEKHIREADMLCRYGGEEFCVVMPNADLEAGMAIAERIRSMVASTA
                     GSMIRNTDVGTITSSFGIASITDGAATIEDLIEQADTALYASKKGGRNRTTAWSPGSP
                     SMEEMDAVAAAAAAEPPRESAPAAS"
     gene            complement(11815..>13631)
                     /locus_tag="PKX01_12700"
     CDS             complement(11815..>13631)
                     /locus_tag="PKX01_12700"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HMZ77034.1"
                     /translation="TSNGGGSSATINVAENTTAVTNVTATDADLPSQTLTFTLAGGAD
                     MARFSIDANTGALSFIAAPDYEAPTDANADNVYDLIVQVSDGQRTGTQTLAIHVINVN
                     EAPVATADAFAGNEDAVLTGNVLGNDADVDGDALSARWVSGPAHGTLVLGADGRFTYT
                     PDANFNGADSFSYVAHDGALDSAPVTVSLHLAPVNDPPVSGVVTLAPHPEDGGPRLIT
                     QAELLSAANDVDGDPLVARDLAIASGRGTLSDNGDGTWTFVPASNDDTRVGFSFVISD
                     GHAETPATAQLSLIPVNDAPAAADAAFATQDTQRHVGQLPAASDVEGDPVVYGLVTAP
                     IRGTVVVQPDGRFTYTPTPGNGGSDAFSFAVTDVHGASTTHTVTVVITVAGGGSGAGT
                     GTEGDTSSDGATSTGRKTRDTTPGVVKTTAPRTATQAESGAAAQVSRIVTAALRGPQE
                     APASDAVVAPASSGSPPAMHHLRRGANGADLADWENIRFSWFSSYSMDFRHSRGSAVQ
                     DWTPATGHPGGREEGEALLETLPAKVAGVSLSVGVVWWAARAGGLLASLAASLPAWRG
                     VDMLMVLKDREPDDDEAGDEDHPGGLPGADDRDERSDG"
BASE COUNT         2151 a         5082 c         4168 g         2230 t
ORIGIN      
        1 gacgacgacc cgctcgtccg ccggttcgcg tcggacgggc tgtgggagat ctgggggcgg
       61 gccggcaccc cggagcagaa cctgaagctc gaacaggccg ggcggctgac cgacccggac
      121 gccgagcggg ccgcctgggc gcacgcccgc gggcacgtct tcacccatcc cgcgctggcc
      181 gccgcagcca ccgtcctcga cgcgctgccg cccgcccgcg ccctcgcccg ccacgccctg
      241 ctgcgcgccc tcgacacctg gcaggccgat ggccgcagcg gcacctggcg cgacttctcc
      301 cacttcgccc ggggcagcac caaactgatc ggtgactccg aacgcgcctg gctcgctgcc
      361 cacctcaccc tggccgactt ccgcctggac gaccacacgc ccctcctcct gctgcgcacg
      421 gcggccgaac tccgcctcgg cgacacccac ctgaccctcg ctgcgatccc ggacttcgtc
      481 gccctgtcgc ctgacaccct gcgccaactc acgggcgccg cgtccggccc cgccgtgtgg
      541 ttcgtggtgg agaaccgcag cagcttcgag cgggtcgccc gcgcctgcgg gcccgacgag
      601 ggcgccctgt ggctgcccgg ctacccgcca ggcagctggc gcgcgtccgt cgcaggactg
      661 ctccgccatc tccccgctcc cgcccgcatc gcctgcgatc ccgacccgga tggcattctc
      721 attgcctgcc aggctggtac gctgtggacg gccgccgggc tcgactggac gccctggcgc
      781 atggacgcgg cagacttcga cgccctcaaa accacgcgcc ccctcacgga acgcgaccgc
      841 aagctgctgg acagcctgct tcacgacccg acgctgcctg cccccctcac ccacctcgcc
      901 aaggccctcg ccacccgcgg cgccaaaggc gaacaggagg cctacctgta agcccctgat
      961 gcgcctcaca aagggaattg cgcaagacgc ttcagaatct cgctgacacg acgttattcc
     1021 gcctcacgaa cccacgcacc ctgcgtcgtc catgcccacg tcctctgcgg cgttacccgc
     1081 cccctcccag gtgctccagc tgctcgccgg agccgaggcc ctgtcccgcg agcgggacct
     1141 gcggaccctg ttccgcctgg gggccgagta cgccatgcgc ttcctggacg gacgcttcgg
     1201 cctgttcttc caggtccgct tcacgccacc ggaactcacc gtcggtctgc tcggctgctg
     1261 tgtctcggat ggcgccgata cccactggga cgatgtggcc gaggcccagt cctggctgcc
     1321 agtcgatccg ctgctggcag tctgcctgca tgccgaccat ggccatgcca tcgagcagtc
     1381 cggcgacatc tggcgcgtcg cctttgccgt cggcgccctt ggcgacgccc gctgggccct
     1441 tgagatccag acgccgcgca agccgtcaaa aaccgatggc gaagccctct ccctgttcct
     1501 gcgctgcttc gagaacgtcc atcgccagtg ggaatacgcc aacctggaca ccctcacgcg
     1561 cctgctcaac cgcaagacct tcgacgacga cttcgaccgc ctcatcgccg ctgcggaact
     1621 cgccgagcag gcccgccagc agcctcagga ccggcgggat ccgggcctca agatcaccca
     1681 gccgtgctgg ctcgccgtgg tggacatcga tcatttcaag cacatcaacg acacctacgg
     1741 ccatctcttc ggtgacgagg tcctgctgcg cctcgccgac ctcatgcgcc agagcttccg
     1801 caaccacgac aagctgttcc gtttcggcgg cgaagagttc gtgatcatgc tgcgcaacgt
     1861 ctcggaggac cacgtccagc ccatcttcga ccgcttccgg atggccgtgg atcgcaacga
     1921 ttttccccgc gtcggccatg tggcctgctc catcggcttc acccgcatcg acccgcgctt
     1981 ctcgcccgcc gagctcctcg gccgcgccga cgaggccttg tacttctcca aggaacacgg
     2041 ccgcaatcag gtcaatggct tcgacaccct gatcatgagc ggccagctgc gcctgtccac
     2101 cccggccggc caggcccaga tccagtccga cgtggacgag atcttcctgt agcggccgca
     2161 ttcccgcctg cccacccggc cgcacgcagg tccttcgggc ccaccgcgcc gcggccggcc
     2221 gcgacgtgcg cccccggatg tgtcaaaatt cagggtttta cggcattccc ggcgcaccgt
     2281 ctgcgccccg ccgaccccga ttccgttgat tgaagagccc atcatgtccg accagacctc
     2341 cgcgcccgcc caggacgaaa accacctgat cgccgaacgc cgccagaagc tcgccgaatg
     2401 gcgcgccacc ggcaaggcct tcccgaacga tttcgcccgg gagaacaccg ccggcaaact
     2461 cgacgaagtc tatggcgaga agagcgccga ggagcttgag gccctgcctg tcgaggtcaa
     2521 ggtcgccggg cgcatcatgc tcaagcgggt gatgggcaag gccagcttcg ccacgatcca
     2581 ggatctctcc ggccgcatcc agctctatgt cacccgcgac ggcgtgggcg aggacgtcta
     2641 cgcccagttc aagcactggg acatcggcga catcgtcggc accgtgggca ccctgttccg
     2701 caccaagacc ggcgagctgt cggtcaagag caccgagctg cgcctgctga ccaagagcct
     2761 gcgccccctg ccggacaagt tccacggcct ggccgacgtg gagaccaagt accgccagcg
     2821 ctacgtggac ctcatcatga gcgaggagtc ccggcgcacc ttcgtggccc gcagccgcct
     2881 cgtccagtcc atccgcaact acatgaccgg ccacggcttc ctcgaagtcg aaacgcccat
     2941 gatgcacccc atccccggcg gcgccgcggc caagcccttc gtcacccacc acaacgccct
     3001 cgacatgcag cagttcctgc gcatcgcgcc ggagctctac ctcaagcgcc tggtggtggg
     3061 cggcttcgag aaggtcttcg agatcaaccg caacttccgc aacgaaggcc tctcgccccg
     3121 tcacaacccg gaattcacga tgatggagtt ctacgaggcc tacgcggact accgcagcct
     3181 catggatttc accgagggcc tgctgcgcca cgccgcacgg gaagccctcg gcaccgaggt
     3241 gttccagtat cagggccgcg aactggatct ctccaggccc ttccaccgcc tcaccatcgt
     3301 cgaagccatc cgcaagtacc accccggctt taccgaggcc cagctcgccg acgcggagtg
     3361 gatcaagaca aagatcgtgg cctttggcga gaaggtcaag ccgggcggcc tgggcagcct
     3421 gcagctccag ctcttcgaag cctgcaccga agccgatctg tgggaaccca ccttcatcat
     3481 cgactacccg gtcgaagtga gcccgctggc ccgcgcctcc gacaccgacc cggagatcac
     3541 cgagcgcttc gaactcttca tcgtcggccg cgagatcgcc aacggctttt ccgaactcaa
     3601 cgacccggaa gatcaggccg cccgcttcca ggcccaggcc ctggccaagg acgccggtga
     3661 cgaggaggcc atgtactacg acgccgacta catccgcgcc ctcgaatacg gcctgcctcc
     3721 caccggtggc tgcggcatcg gcatcgaccg cctgatcatg ctgctcaccg acagcccggc
     3781 gatccgcgac gtcatcctct tcccgcagat gcgccccgaa gcggactgac acgcagcccc
     3841 gcgcagcccc cttgcgaggc ccgcccggcg tctgcccggc gggcctcgcc gcatccgcgc
     3901 caggggcagg acagggccac gccagacatc agacaggccg cccgctgcga acccggaagc
     3961 gccccatcag gtgcttgaca tggtccgcct gtttggtcag ggccgccgcg gcctcactgc
     4021 attccacggc gtggccgttg gttcgctggg cgactccgtc gatctgcccc agccccgcgc
     4081 tcacttcact gatgccggat gcctgatcct gggacgccgt ggcaatctgg gcgaccagtt
     4141 cggaaacctc gctcgcgctg gtgacgatga gcttcagcgc atcggccgtg cgccgggcga
     4201 tctcgatgcc atgggacgtc cgggcgcccg attcgctgat gagctgggag gcctgggccg
     4261 cagcctccgc actgcgcgac gccagcttgc gcacctcgtc cgccacgacg gcgaagcccc
     4321 ggccggtctc gccggcccgc gccgcctcga tggccgcatt cagcgcgagc agattggtct
     4381 gattggtgat ttcattgatg gcattgatga tgccgttgat gcggctgctg gcatcgctga
     4441 tggactggat ggccgcagtc agctcgtcca tgtgctggct gcccaggctg gcggcgtccc
     4501 gtgagcggat ggagatcgtg ttggccatgc ccgagttctg ggcgttattg cgggtctgca
     4561 cttccatctc ggaaatcgcc gccccgatct cggtgacgga ctcggccgac tggccggcac
     4621 cgtcggtcag gaccgcactc aggtcggcca cctgactggc gcctgaggcg atcaggttgg
     4681 cgccgccctg cagttcggaa accagattgt tcaaggtatc caccatccgt ttcaaggcgc
     4741 gtccgagctg atcgtcgtcg gacgcgagct cgacgtccac attgaggtcg ccttcgctga
     4801 tctgctcggc gagcctggcc ttgctcagca gcccctggga catctcgtcg agggcgtggg
     4861 ccaggtgccc cacttcgtcc tcggaatggt gctgaaagcg cttcgagaag tcgccgcgac
     4921 ggatcagccg ggcgaactgg gccgcttcgc ggatggggcg ggcgatggaa ccggccgcaa
     4981 accacaggat ggccgtggcg ccgacgctga tggccagccc ggcaacgatc agcaggatcg
     5041 tggagctccg tccacgcgcc tggatgtcgt cgtcgagctg ctgcgcctgg gccagcacga
     5101 ccttcgtcgg cacccgcacc agtaccgccc acggcttgcc ggtgcggccc agggcaatcg
     5161 gtgccaccgc gctcatgatc cccgagccct catcggtcca gccgcgggcc ttgccgctct
     5221 ggatgtcggt catcaggttg gcgttggcag gtccgttgag ctcgagacgt ttgccgatga
     5281 gctcgggatg ctcactgtcg gccaccacca gccccatgtt gctgacaatg gtgacctcgc
     5341 cgcggccctc gaacagcccc ttgtcggttt cctcggcaag ctgctggacg aaattgagat
     5401 tgtagtcagt cccggcaacc ccataaaact tgccgtccac catgatcggc accgaaagcg
     5461 tggtgagcca cacctgcttt ccctgcacga tatacgggaa cgggtccagc acgctttccg
     5521 tatgttgatc acgggggccg atataccatc cgcctttcaa tacaccgttg ggatgtttgt
     5581 ccagggtgtc gtattcaacc agaggctgca ccgcgatgcg ccccccctcg ccacgattcc
     5641 aatagggcgt gaagcgcccc gtcttggcat tgtcgccgtt caatccggtg gcatgagcgg
     5701 cgtcctgtcc gtcgagggcg ttgggctccc agcatgaata cgtcccgttg aactccgggt
     5761 tgttctccag cacgttcttg aggattgcat tgatctgtcc ccgccccagg ctgaccccgc
     5821 tgcccggagt cttggcgaga acgaaggtat gggccatggt ccgcgccgcg tccagcgcca
     5881 ggtcgaactt ggcctgaatg cgccccgcct gcgtgcctgc gaggtgtttg aggctctcgg
     5941 cggtatcgtt tttgaccagc gtgctgaccc gctctgcaac gacactcttc gtggcatcgg
     6001 acgagatgac gctgaaggcg accagggcaa tcgcggaacc gatcagacaa ctccctgcag
     6061 ccatggaaat acgcccttga atggatttcg ctttcatttt tggtcttctt gctaattgaa
     6121 tagttgtcca ggacgaatca acaacacaga caaggccgcc atgcattcat gcgccggggc
     6181 caaattcata tcggcgacac attaaggcgc tttagactca taaataaaat ttttatcgcc
     6241 aacccccatc ccggcaaggg ctgtccattt attaaacaaa ctccttttac gttcgcgtca
     6301 attacatagc gcaattcgac aaacaattgt ccattaatta aacaactacc catttgattg
     6361 atccggatca attcatgacc atcaattctc tgaccacgga attcggattc gcaaggaatc
     6421 ggcaatcggt ctctgcgccc ggtgggaccg tgcgcttcgc gcgccctcct gcgccagcgc
     6481 ccgaagagaa tcgggggaga ctgcccgacg gaccggtctc cgcagacatg ccacctgcgt
     6541 ccatggaccg ccagccgcat cccacgctcg acatttggta gcatcaccag gatcttggta
     6601 gaataaccaa acccttgcag acctccgcgc catgtccgtc cgtgaccgcc tcctcgatgc
     6661 cggcgtgagc ctcctccgcg atcaaggcgc ctctgccctg acccagccgc gcattgccgc
     6721 ggccgccggg gtcagccaga gccacctcac ctactacttc cccacccgcg acgccctcct
     6781 gctggccatc gcagagcatt cggtcgatca ggaactccag cgcgccaccg ccgacagcgg
     6841 cggccatccg gccgaacgcc tggcccaggc ggcgcgtttc ctgccccggg tgcgcatgat
     6901 gctgggcctg gtcgcctcgg gagaccggga cccggccatc cgccccgcct ttgcacgcct
     6961 cgccacccac gtgcgcgagg ccctcggcca gctcctcgcc catcacggct acaccgtcag
     7021 cgcaccccag gtggtggtgg tccatgccgc cctcgtgggg ctggccatca tgaacctcgg
     7081 ccgccaggac gccacctccg acgccgacgt ggagcttggc ctgcgccagc tcctcgccct
     7141 cctgccccat ccggaaagga ccgaaccatg acctcgcgcg cgtgcctccc gatcgccttg
     7201 agcctggccg tggccgcctg cggcacgccg gagcggagcg acttccaggg ctacgtggaa
     7261 ggcgaatacc tctatctcgc ggcgccccag gccggctatc tcaagcgtct ggatgcgccc
     7321 cgcggccgtc gcgtcacaga cggcgagccg ctcttcgaga tcgccgccga cccggacgac
     7381 caggccctcg ccgaagccca ggcccgcgcc ggcgcggccc gcgaaaagct cgagaacctg
     7441 cgcgaacccc gccgcacgcc cgagatcgcc gccctcgaag ccgcgctcac cggcgccgtc
     7501 gccgcacgcc gtctggccga ggtccagctg cagcgccagc aggccttgtc gcgccaacag
     7561 ttcgtctcgc cggccgccgt agacgacgcc cgcgcccgcc gtgatcaggc cgtcgccggt
     7621 gaagacgccg cccgccagca actcgccacc gcccgcatcg ccctcggccg cgcgccggaa
     7681 gtgcgcggcg ccgaagccga tctgcgcgcc gccgacgccc aggctgccca gaaacgttgg
     7741 gccctcgagc gcaagaccgt cgccgcgccc accgcaggcg agatcagcga cacctactat
     7801 cgccccggcg aatggattcc cgccggcgcg ccagtgacca gcctgctacc ggacacgcga
     7861 cggcgcctgc gcttttacgt acccgaaacg gcccttgccg gcctgcaacc gggccaggtc
     7921 gtggaagccc gctgcgacgg ttgcgcggcg ccgatccgtg ccaccatcga cttcatcgcc
     7981 ccccaggccg aatacacccc gccggtcatc tacagccgcg gcacccgcga gaagctggtg
     8041 tttcgcgtgg aagccgcgcc agcgcccgac gacgcgcgcc gcctgcggcc cggtctgccg
     8101 gtggacgtgc gcctcgcgga gcgccgcgat ggctgactgg gtcatcgacg tgtgcggact
     8161 caacaagcac tttggcgccc gtcatgtggt gcgcgacctc agcctgcagg tccgcgccgg
     8221 cgagatcttc ggcttcctcg gccccaacgg cagcggcaag accacctcca tccgcatgct
     8281 gtgcggcctg ctcacgccgg acagcggcgc cggcacctgc ctgggccacg acgtgatccg
     8341 cgagagcgcc gccatcaagc gccaggtggg ctacatgacc cagaagttct ccctgtggga
     8401 cgacctcagt ctggccgaga acctggactt cgtcgcccgc atgttcggca tggacgaccg
     8461 ccgcgccgca gtggaccgca ccctcgtgga gttgggcctg gagggccgcc gccaccagct
     8521 ggcgggcacc ctctcgggcg gctggaagca gcgcctcgcc ctcgccgcct gcctgctcca
     8581 ccgacccagg ctgctcctcc tggacgaacc caccgcgggc gtagacccca aggcccggcg
     8641 cgacttctgg gaggagatcc accgcctctc cgccgccggc atcaccatcc tggtgtccac
     8701 ccactacatg gacgaggccg agcgctgcca ccgcctcgcc tacatcgcct acggcaacct
     8761 gctggcttcc ggcaccgccg ccgaggtggt ggccggccgc ggcctgcaca cctgggcggt
     8821 cagtggagcc gccagccacg gcctggccga caccctgcgg cgcctgcccg gcgccgagca
     8881 ggtggccagc ttcggcaccg ccctgcacgt gagcggcacc gacggtgcgc gcctcggcac
     8941 cgccctggat gagctgggcc gccgcggcga tctgcgcatc gaggcggtcc agccctccct
     9001 ggaggacgcc ttcatccacc tcatgcagca cagccaggac cccctcgcgg gctgacggcc
     9061 atgggcattc cggaccgctt ctccttctcc cgctggctgg gcatcctcat caaggagttc
     9121 atccagctca agcgcgaccg cctcaccttc ggcatgatcg tcggcatccc gatcctgcaa
     9181 ctgctgctct tcggcttcgc catcaactca gatcccaggc acctgcccac cgcagtggtg
     9241 atggccgacc cgggcccctt tgcccgcgcc tacgtggcgg ccctgcgcaa cagcgactat
     9301 ttcgacgtgc gcggcagcgt cgacgcacgc gaggccgacc gcctgctgga gcggggcgac
     9361 gtgctcttcg tggtgagctt tccgccgggc ttccatcgcg acctggtacg cggccgcagc
     9421 cccacggtcc tggtcgaggc cgacgccacc gacccgatgg ccaccgccgg cgccatctcg
     9481 gtgctcaaca agctcggcgc ggaggtcttc gcggcggatc tgccggggct ggagcgcccg
     9541 cccgccgcgg ccatcgacct gcgcgtgcag cgccgctaca acccggaagg catcaccgcc
     9601 tacaacatcg tgcccggcct gatgggcgtg atcctgacca tgaccatggt gctcatgacc
     9661 ggcctggcca tgacccgcga gcgggagcgc ggcaccttcg aaaacctcct cgccacgccg
     9721 gccacgccgc tggaggtgat gaccggcaag atcgtgccct acatcctcat cggcctgatc
     9781 caggtcagcc tgatcctcgc cgccgcccgc tggatcttcg acgtgcccat gttcggcaac
     9841 ctgctggtgc tctacgcggt ggtgctgctc ttcatctgcg ccaacctgac cctgggcatc
     9901 accttcagct cccttgcccg caaccagctg caggcgatgc agatgacttt cttctttttc
     9961 ctgccctcca tgctcctgtc cggcttcatg tttcccttcc ggggcatgcc cgagtgggcg
    10021 caggcagtgg gcaacctgct gcccctcacc catttcctga tcctggtgcg gggcatcctg
    10081 ctcaagggga atggcgtgct gtgggaacag gtgtggccca tcctcgcctt catgggtgcc
    10141 gtgctggcgg tgggcctcaa gaccttccgc cggacgctgg actgacgcgc acgcgccggg
    10201 cgtcaggcgg gcgacatcac gacgcggccg gcgcggattc ccgcgggggc tccgcagcgg
    10261 ccgccgctgc cacggcgtcc atctcctcca tgctgggcga acccggcgac caggccgtgg
    10321 tgcggttgcg gccacccttc ttggaggcat acagcgcggt gtcggcctgc tcgatcaggt
    10381 cctcgatggt cgccgcaccg tcggtgatgg aggcgatgcc gaagctggac gtgatcgtgc
    10441 ccacgtcggt gttgcggatc atcgatccgg cggtgctcgc caccatgctg cggatgcgct
    10501 cggcaatggc catgcccgcc tccaggtcgg cgttgggcat caccacgcag aactcctcgc
    10561 ccccgtagcg gcacagcatg tcggcctcgc ggatgtgctt ttcgatgcag cgcacgacca
    10621 ccttgatgac ctggtccccc accgcgtgac cgtagatgtc gttgaagcgc ttgaagtggt
    10681 cgatgtcggt catcaggcag cacaactggc cattgccgcg cagggccgtc tcgaagggcc
    10741 cggccacctt ctcgaagaag gcgcgccggt tgagggcgcc ggtcagcgga tcgcgggacg
    10801 ccaggtgggt cagcttctcg ttctgggcct tgatgcgggc gcgcgattgc tcgatctccg
    10861 acagggcctt gcgcaggtcc tggttggccc gctccagctc cgacagatcg tcgaagctca
    10921 ccaggcagcc gcgggtgttg ccgtattcgt cgcgcaccgg cgagcaactc accgccagtt
    10981 ccagcgtccc gccgccggga cgcaccacgc tcatgcgcac gtccgtgacc agatcgtcgc
    11041 cacgcagcac ccgctcccac ggggcgcgcc ccttctcgaa ggactccatg cccgcgtcca
    11101 gccagtccag atcggcgatg cgccggccga tcaggcgggt cggcgactcg ccgtggagct
    11161 gccggaaggc cttgttggcg agcacgatgc gtccgtccgc gtccaggacc agcagcgcgt
    11221 ccgtgagcgt gtcaaaggcc ttgcggaccc gctccggcac tgcgctggac ggatcgagga
    11281 actgcatcgc ccgccgcagg taggcgtaat acacgagaaa cccgcccacg ctcaccagca
    11341 ggatgatccg cgtcagcggc tgggccagcc acgccccggc gccgggcgcg tgggtcggcg
    11401 caaaactgat ctccacatcg ccccaatggc tgcgcccggc attgagcggt acccggagat
    11461 gctccagcgt ggaccggcca gactccggcg cgatccagtg cacggcatgg tccccccgct
    11521 gggccatcag cgtgccgtcg gaacgccgga cagccagcga gcgcagctca ccgttccgcg
    11581 tcagtatcct ttgcagcgtc cggccaagcc cgagttcgtc gttcgcctcc accagcaccg
    11641 tcagttgggc cgccagattc tcaccctcac gccgacgggc ctcctggatc attgcggtct
    11701 cgctggatgc gaacccgaac agccagtccc ccgccatcag cagcgtcagg gtaagcgcca
    11761 ccagccccag actgaggcgg gccaccggtc ccacgcgcgg caatacgctc ttcttcatcc
    11821 gtcgcttctt tcgtcccgat catccgctcc cggcagaccg ccggggtgat cctcgtcccc
    11881 cgcttcatcg tcgtccggct cccggtcctt gagcaccatc agcatgtcca cgccgcgcca
    11941 ggcaggcagc gaagccgcga gactggcgag cagaccgccc gcgcgggctg cccaccacac
    12001 caccccgacc gacagggaca cgccggccac cttggcaggt aaggtttcaa ggagtgcctc
    12061 gccctcctcc cggccgcccg gatggccggt ggcgggcgtc cagtcctgca ccgccgaacc
    12121 gcggctgtgc cggaagtcca tggaatagct ggagaaccag gagaagcgga tgttctccca
    12181 gtccgccaga tcagcaccgt tcgccccccg gcgaaggtgg tgcatcgccg gggggcttcc
    12241 cgacgatgcc ggtgccacga cagcatcgct ggcaggcgcc tcctgcggcc cccgcagggc
    12301 cgccgtgacg atccggctga cctgggcggc ggcgcccgat tccgcctgcg tcgccgtgcg
    12361 gggcgccgtg gtcttgacga caccgggcgt cgtatcgcgg gtcttccggc cggtgctggt
    12421 cgcgccgtcg gaactggtgt ccccttccgt tccggtgccg gcccccgacc cgccgccggc
    12481 gaccgtgatc accactgtca ccgtatgggt ggtcgaagcg ccgtggacgt ctgtcacggc
    12541 aaaactgaac gcatccgacc cgccgttgcc gggcgtcggc gtgtaggtga accgcccgtc
    12601 aggctgcacc acgaccgttc cccggatcgg cgccgtgacc aacccgtaga cgacgggatc
    12661 gccttccaca tcgctggcgg ccggcaactg gccgacatgg cgctgggtat cctgggtcgc
    12721 aaaggcggca tccgcggcag ccggcgcatc attgacgggg atcaggctca actgcgcggt
    12781 cgccggcgtc tctgcatgtc cgtccgaaat cacgaaactg aaaccgaccc gggtatcgtc
    12841 attggacgcc ggcacgaagg tccaggtgcc atccccgttg tcgctgagcg tgccccgccc
    12901 gctggcaatg gcgagatcgc gcgccaccag ggggtcccca tccacgtcgt ttgccgcgga
    12961 cagaagctcc gcctgcgtga tgagacgcgg cccgccatcc tcgggatggg gcgccagggt
    13021 caccaccccc gataccggcg gatcgttgac cggcgccagg tgcagactca cagtcacggg
    13081 ggcactgtcc agcgcgccgt catgggccac atagctgaag ctgtcggcgc cgttgaagtt
    13141 ggcgtcaggc gtgtaggtga aacgcccatc tgcccccagc accagcgtcc catgggccgg
    13201 cccggacacc caccgcgccg acagtgcgtc gccatccaca tcggcgtcat tgcccaggac
    13261 gttgccggtc agcaccgcgt cctcattccc ggcaaaggca tcggcggtcg caacgggtgc
    13321 ttcattgacg ttgatgacat gaatcgcgag cgtctgcgtt ccggtccttt ggccgtcgct
    13381 gacctggacg atcaggtcgt agacgttgtc ggcattggcg tcggtcggag cttcgtagtc
    13441 gggtgcagcg atgaagctca gcgcgccggt gttggcgtcg atgctgaagc gggccatgtc
    13501 tgcgccaccg gccagggtga acgtcagcgt ctgcgagggc aggtcggcgt cggtggcggt
    13561 cacgttcgtc accgcggtcg tgttctcggc cacgttgatg gtcgcggagc taccgccgcc
    13621 gttgctggtg a
//
LOCUS       DAOQPA010000095        13622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2333_length_13622_cov_22.128400, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000095 DAOQPA010000000
VERSION     DAOQPA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_13622_cov_22.128400"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..807
                     /locus_tag="PKX01_12705"
     CDS             151..807
                     /locus_tag="PKX01_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77035.1"
                     /translation="MNVVVNHQQELFVVPAGQGVSTLGFEYVFGQLKQLVARLNLPIT
                     VREDEKGTIGQYADYQRAIGEARKANLKETWFHLNTPVEVRRILERYRKSGNPIRIFY
                     GDTATGRDWLEENDVVGIVARSCGIFKVPILLASSESWGNGILDHCIVRLMDTGSRRV
                     LWTHPKYQAPVMQIAAERQGSYTHAVFVSDELHARFPSYAKAAQWVAFMAGECMEAPR
                     "
     gene            903..1307
                     /gene="ssb"
                     /locus_tag="PKX01_12710"
     CDS             903..1307
                     /gene="ssb"
                     /locus_tag="PKX01_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ77036.1"
                     /translation="MLNKVQLIGFLGADPDVRYTQEQEAVARVRVATSETWKKNGEKH
                     ERTEWHNVVFFGKLAEIVGEHLKKGSLVYVEGHLQTRSWEKEGETRYTTEVIAEGMKM
                     LPSGKAKADSDAPRSTPRNQTKAKQTHDDGPF"
     gene            1467..2570
                     /locus_tag="PKX01_12715"
     CDS             1467..2570
                     /locus_tag="PKX01_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HMZ77037.1"
                     /translation="MTKEELDDLKKEVLRMAHGRWGGILGALAPQLQAALDRVGRHVP
                     CPVHGGKDGFRVFKDVDETGGSVCNTCGINSDGIATLIWANGWRYSTTLQSVADYLRI
                     GARPARPARRVTKPAPAAGEDDEKLRQSLNRVWNESAPISNRDAEPARLYLARRGISI
                     SPPEALRFHPALAYYDGEKRIGEFPAIVGLVSGAQGDPVTIHRTYLTSDGKKAPVESP
                     KKLMAYPKERKIVGGAIRLTDAHSQVMVVAEGLETSLAVMEGTGLPVWCAINALLLAN
                     FVPPGSVTRVLVFADKDRPTDQHPRGHGQEAAKQLVQRLWGMGIQASAIVPAGEIRPG
                     QKSLDWLDILNRDGKAGFPSLKSIDGRVMRCAA"
     gene            2584..2826
                     /locus_tag="PKX01_12720"
     CDS             2584..2826
                     /locus_tag="PKX01_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77038.1"
                     /translation="MYGIIDFSVIDTEPGAASAFAPPAGFEGDYCEYMRGRWKHDVGV
                     RQHIAVVGRMLVREETVMFSGPFADAARSIAEAFWQ"
     gene            3057..4100
                     /locus_tag="PKX01_12725"
     CDS             3057..4100
                     /locus_tag="PKX01_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77039.1"
                     /translation="MATVSIRPQQAANLVGRYIDARIPVLLTGAPGVGKSSIVEQAAK
                     AAGYDVILSHPVVADPTDAKGLPWPGKDGESATFLPFGDLARAIKATTPTVWFLDDLG
                     QASPAVQASFMQLLLARKVNGHALPGCVTFVAATNRRSDRAGVSGILSPVISRFGAVV
                     GVETSVDDWCEWALGADMPPELISFIRFRPNLLHLQDTRKADDMENFPCPRTWEYAGK
                     AIQLGLAEDLEFAAFAGAVGEGAAIELRGFLTMVRSKLPNPDSIILDPDNAPIPGRPD
                     LLWAVTTALAKRASDTNCGRIFRYAERLFDDGKSEFAVMMVKDINRLTPSVRDTQDWT
                     RLVLTNLGKSFLA"
     gene            4178..5035
                     /locus_tag="PKX01_12730"
     CDS             4178..5035
                     /locus_tag="PKX01_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77040.1"
                     /translation="MIPNLSKKAMLVGLAIRGWQARKYDRKISIEVAEQHAATPDAGR
                     FNKHLLPGNAESYEAVHKKGRELRAFYYENTLPWSKEGQRILPAANFEDFAEGVRRFK
                     REYAMLVEEFLREYPILKEDARVLLNGMFNEMDYPAVEDMRARFSIELETLPLPAAED
                     FRVALADEEVARIQRDIEKRLQHEFANANQDLWNRLRHAVDNMVVRLSNPEGRFHDTL
                     VSNLQSIVELIPKLNFTGDRNLEQIGATCAEALAVHDPQALRDDAILRARVAAQAKEI
                     SSIMDAYMS"
     gene            5092..6306
                     /locus_tag="PKX01_12735"
     CDS             5092..6306
                     /locus_tag="PKX01_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HMZ77041.1"
                     /translation="MKAKEYVSAARRNLVLDHPFFGVLSLKLDLIEDRSVKTLRTDGR
                     KLWFNPEYASSLNRYEIVGVVAHEVLHCANGHAWRRGDRDPKVWNQACDLAINPIVTG
                     AGLVLPEGALDGSAYKGMSAEEIYGRLVQQAQDQQREEEDSSDGGGGQQGGQSAQGDD
                     SGDDTQDGQDGGGEAPRPDGSECGEVTDCEADQTPEIQADWSAAVLNAAKQAEAMGTL
                     PAGMQRLVEEIKNPPRDWRSILRRFVQTSASHDYSWRQPNSRYLYAGLYMPALRAESM
                     PPMVIAVDTSGSIDDLTVSQFAKEINAIADEVQPEQVHVVYCDAEIHGVDVFERGEPI
                     AINPKGYGGTDFRPVFEWVANEGLNPSCLVYLTDMAGYFPESAPDYPVLWGDTSGMLA
                     APWGETVRIRCN"
     gene            6547..6903
                     /locus_tag="PKX01_12740"
     CDS             6547..6903
                     /locus_tag="PKX01_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77042.1"
                     /translation="MDKETIKNFIHWLDQAGDEELERNRQYILAKMKLVSTSEGRAVF
                     KLALRLLDEEVLCGPSPDIVRLVRQRDALLAALNHIEVDKDGDGFICREAMDEVRQAI
                     ALATEVAAQAVRTPAV"
     gene            6991..7590
                     /gene="radC"
                     /locus_tag="PKX01_12745"
     CDS             6991..7590
                     /gene="radC"
                     /locus_tag="PKX01_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMZ77043.1"
                     /translation="MNLESMDNTDLLAMLVGRRLARSLSGRPLAEIFGYCRPRQPALG
                     EQPVPYVAHPAIGAAKELFTRCILERMAVEDLCFSSPETVQAFLCSRIGHLEHEVFWS
                     FWLDAQNRLIVAEQLFQGTLTQTSVHPREVVKKALARNAAAVIFAHNHPSGVPEPSRA
                     DEMLTTALKSALALVDVRVLDHFIAAGNQAMSFAARGLL"
     gene            7590..7865
                     /locus_tag="PKX01_12750"
     CDS             7590..7865
                     /locus_tag="PKX01_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77044.1"
                     /translation="MSAFKPIRQELALVIDLDERGWFKAHVGDQNGKTVFQFSNEDEE
                     TGWPGEAGLWLVEDGFMRHGRDTDGLLTYLKSIGIAKPTATMSLCNY"
     gene            7951..8442
                     /locus_tag="PKX01_12755"
     CDS             7951..8442
                     /locus_tag="PKX01_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77045.1"
                     /translation="MKKLIITLGQDLSAYATIEVPAETDLSDESLAAIARKAVEDEGT
                     VFDEDWSTVNALRIVLVQDEKGQTIRSDIPVESSCYEGGAALQLFLKGGIGVEALVNS
                     AMKAKLIDPARNGAEVAAIAAQADLLAAAEAALARLKEWSDFAPEIGLLEAAISRVKG
                     RSS"
     gene            complement(8918..9253)
                     /locus_tag="PKX01_12760"
     CDS             complement(8918..9253)
                     /locus_tag="PKX01_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ77046.1"
                     /translation="MQKRIFEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVAEIRKAM
                     RALGLTQQAAAKRMGIPQPKVSAMMRGDFTNLSERKLMDCLNRLGYDIEIKVRPAAGP
                     IGHLTLATA"
     gene            complement(9234..9611)
                     /locus_tag="PKX01_12765"
     CDS             complement(9234..9611)
                     /locus_tag="PKX01_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ77047.1"
                     /translation="MADKEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQMGDQDDA
                     AKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAVFVLHCFQKKSKSGIATPKTDMD
                     IIRARLRVAEAMAQELRNAKTNL"
     gene            complement(10161..10379)
                     /locus_tag="PKX01_12770"
     CDS             complement(10161..10379)
                     /locus_tag="PKX01_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HMZ77048.1"
                     /translation="MRIEFRSDTRGYILALERDLFGAFVLVRRWYGLGNRRGGVKREV
                     FLQEDAALRELKRIERLRVRHGYRRLEE"
     gene            complement(10369..12270)
                     /locus_tag="PKX01_12775"
     CDS             complement(10369..12270)
                     /locus_tag="PKX01_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ77049.1"
                     /translation="MPPVERKVGRHHLALYRGWLQGLDLKALADRYLETGLDLRLAKA
                     TLVWLRDTLSQAALRHGHRGEARLLRLHLAPGQQAKALPCPSLDDFRAEHDPGGFYRE
                     EELIQLYLDAFPEVRDKRGRQRQRLIDRQLAALVWIERLLVTDPVPADLVSAWFDQPI
                     ADRLILAGIPTVGALLERIRGRGYRWWVTVPKLGEKGANRIVAWLRGYESSLGALPGH
                     ALAPVRTQPVPALIRERNRETAIVPMEAFVVPEALAGATGSNRYPGQPRIQAVNDLQA
                     IQSWLATKSGSSNTERAYRKESERLLLWAVIERQKALSDLTVDDCAAYRDWLSALGRS
                     SPEHWVFRVPQSDWIGKRNTPRFSPAWRPFDGPLSASSVRQALTIVSGLFEWLVRVQY
                     CAFNPLDAVGKKLTTQEDAPADIELTRVFSQAQWRYLTHHLDQLPRDAHSHRLRFVLP
                     FAYATGLRLSELVDAKIGRLYTMPLRDSLGVRWMLKVRGKGGKWRAVPISEGVLIALG
                     EYLTYRGLAPDPLANPSETPLIARLDGNEPVSPSSLYKALRALFQGAADALRAEGHMQ
                     EAKDFDRATVHWLRHTRGNHLGLAGVPPNLIQKLLGHASLATTSIYTESDEERVWQEL
                     ANLEAADAD"
     gene            complement(12613..>13622)
                     /locus_tag="PKX01_12780"
     CDS             complement(12613..>13622)
                     /locus_tag="PKX01_12780"
                     /inference="COORDINATES: protein motif:HMM:NF018148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HMZ77050.1"
                     /translation="SLFSHDSQEVDLNILGIDIGYSNLKLAFGQKGGTPKTHLRPAGA
                     APADRFGSRFDGKAQDDFLHVLVNGQAFVAGVSPDRAELWSRSLHADYASSNSYAALF
                     HAGLLLSEMDRIDTLVTGLPVSQYLDETRRNALAAQMQGTHQVTPKRTVSVERVKVIP
                     QPIGGLLDYIAQEDADIDDARVLVVDPGFFSVDWVVIAHKDLHRQSSGTSLNASSVVL
                     EEASRLIAKDHGSALNVETLENALRAGKATVLVLGQRVEVAPYIEQAAKTVGPVVIES
                     IQKSLRTESKMPDLVVLVGGGAAFFREAVQDAFPRLAVLTPKESVFSNARGFWLMGAA
                     L"
BASE COUNT         2586 a         4188 c         4441 g         2407 t
ORIGIN      
        1 tgccagggga catgaaggta gctggccttc cgaaacaaac agcataaagg cgacagccgg
       61 gtgaccggat gtcatataca accccaccgg ggacgcgtgt ccccgggcga ggggctacgc
      121 gcctccggcg gattctcgtt tggaggtgcg atgaacgtcg tagtgaatca tcagcaggag
      181 ctgttcgtcg ttcccgctgg tcagggagtt tcgaccctgg gcttcgagta tgtcttcggt
      241 caactgaagc agctcgtcgc caggctgaac ctcccgatca ccgtccgcga ggacgagaag
      301 ggaacgatcg gacagtacgc cgactaccag cgagcgatcg gcgaagccag gaaggcgaac
      361 ctcaaggaga cctggttcca cctgaacacc ccggtcgagg tgcgtcgcat ccttgagcgc
      421 tatcgcaaga gcggcaaccc gatccgcatc ttctacgggg acacggcgac gggccgcgat
      481 tggctggagg agaacgacgt ggtgggcatc gtcgcccgct cgtgcggcat cttcaaggtg
      541 ccgattctcc ttgcgagcag cgaatcgtgg ggcaacggca tcctcgatca ctgcatcgtc
      601 agactgatgg atacggggtc ccgcagggtg ctctggacgc atccgaagta ccaggcgccg
      661 gtgatgcaga tcgcggccga acggcaaggc agctacacgc atgccgtctt cgtcagcgac
      721 gagctccatg cccgcttccc gagttacgcc aaggctgccc aatgggttgc tttcatggcc
      781 ggcgagtgca tggaggcgcc gcggtagccc acgattcaac aaccacggcg ggaacgtcat
      841 gcttcccgtc aggggcatgg tgcgaccgct attttcgtct catgacagaa aggagtttca
      901 ccatgttgaa caaagtgcag ttgatcggct ttctgggcgc ggatcccgat gttcgctaca
      961 cccaggagca ggaagccgtc gcccgcgtgc gcgtcgccac cagcgaaacc tggaagaaga
     1021 acggcgagaa gcacgagagg actgaatggc acaacgtcgt cttcttcggg aagctcgccg
     1081 agatcgtcgg cgagcatctg aagaagggtt ccctggtgta cgtcgaaggc caccttcaga
     1141 cccgctcctg ggagaaggag ggcgagaccc gatacaccac ggaagtcatc gccgagggca
     1201 tgaagatgct cccgagcggc aaagccaagg ctgactccga tgcacccaga tcgacgccga
     1261 ggaaccagac caaggccaag cagacccacg acgacgggcc gttctgaacc gagaacgatc
     1321 gttgaacccc ggccggggag gtaatccccc ggtcgggggt gcctcctcgc gctggtcttt
     1381 ctttcacccc ggaacggggc atccccaagt gcctccccgt cgggtgcttg tggatgcccc
     1441 gttccggggc tttccaggag aggaccatga cgaaagagga gttggacgac ctcaagaaag
     1501 aggtgttacg catggcgcat gggcgctggg gaggaatcct cggcgctctc gctccacaac
     1561 ttcaagccgc gctcgaccgc gttggccggc atgtgccgtg cccggtgcat ggcggcaagg
     1621 atgggtttcg cgtcttcaag gacgtggacg aaactggcgg cagcgtctgc aatacctgcg
     1681 ggatcaattc cgacggcatc gcgacgctca tctgggcgaa cggttggcgc tactcgacca
     1741 cgctccagtc ggtggctgac tacctgcgca taggggctcg cccggcgagg cctgcgcggc
     1801 gggtgacgaa gccggcgccg gcggcgggcg aggacgacga aaagctgcgg cagtcgctca
     1861 accgggtctg gaacgagtcg gccccaatct cgaaccggga tgccgagccg gccaggttgt
     1921 atctggcacg gcggggaatc tcgatctcgc cacccgaagc ccttcgcttt catccggccc
     1981 tggcgtacta cgacggcgag aagaggatcg gggagtttcc ggccatcgtc ggcttggtat
     2041 ccggagccca gggcgacccg gtgacgattc atcggaccta cctgacatcg gacgggaaga
     2101 aggcgccggt cgagtcgccg aaaaagctca tggcctatcc gaaggaacgc aagatcgtcg
     2161 gaggcgccat ccggctcacc gacgcgcaca gccaggtgat ggtagtggca gaggggctgg
     2221 aaacctcgct ggccgtcatg gagggcacgg gcctgccggt ctggtgcgcg atcaacgcgc
     2281 tcttgctggc gaactttgtc ccaccgggct cggtgacgcg agttctcgtc ttcgcggaca
     2341 aggatcggcc caccgaccag caccccaggg ggcatggaca ggaggccgca aagcagcttg
     2401 tccaacggct ctgggggatg ggcatccagg catcggccat cgttcccgcc ggagagattc
     2461 ggcctggcca gaagtcgctg gactggctgg acatcctgaa tcgggacggc aaggccgggt
     2521 ttcccagcct gaagtccatc gatgggcgcg tcatgcgctg cgccgcctga aggagatgag
     2581 gccatgtacg gaatcatcga cttcagcgtc atcgacaccg aaccgggagc ggcctcggcg
     2641 ttcgcgccgc cggccggctt cgagggcgat tactgtgagt acatgcgcgg ccgctggaag
     2701 cacgacgtcg gcgttcgtca gcatatcgcc gtggtcggcc gcatgctcgt ccgggaagag
     2761 accgtgatgt tcagcggccc cttcgcagac gcagcgcgaa gcatcgcgga agcgttctgg
     2821 cagtaacggc gccccgccgc aaggcggggc ttttcacagc gcatcatgcg gtgcgctgtg
     2881 aaaaggcaga ttcgagtcgc accgtaccgg tgtcggcaag atgcggtagc tggccgcccc
     2941 gaagggggaa gcagcaatca acgccgttct gggcgatcca gccggcaatt tctccaaccc
     3001 tttgcggggc gctcccctgc aatggggaag ctccgcatct ttcattggga gcttccatgg
     3061 caacagtatc tatccggccg caacaggcgg ccaacctcgt cggcaggtac atcgatgccc
     3121 gcatccctgt cctgctgacc ggtgcccctg gcgtcggcaa gtcatcgatc gtcgagcagg
     3181 cggcgaaagc agccggctac gacgtgatcc tgtcgcatcc ggtcgtggcc gatcccaccg
     3241 acgccaaggg cctcccttgg cccggcaagg atggcgagag cgcaacattc ttgccctttg
     3301 gcgatctcgc cagggcgatc aaggcgacga cgccgacggt ctggttcctg gatgacctcg
     3361 gtcaagccag cccggcggtt caggcatcgt tcatgcagct gctcttggcc cgcaaggtca
     3421 atgggcacgc gctgccgggt tgcgtgacct tcgtcgcggc gaccaatcgc cgttcggatc
     3481 gcgccggcgt ctcaggcatt ctctctccgg tcatctcgcg cttcggcgcc gtggtcggag
     3541 tggaaacgtc cgtcgacgac tggtgcgagt gggcgctggg ggccgacatg cctcccgagc
     3601 tcatctcgtt catccggttc cgtccgaatc tgctgcacct gcaagacacc aggaaggcgg
     3661 atgacatgga gaactttccg tgtccccgca cttgggaata tgcgggcaag gccatccaac
     3721 tggggctggc ggaggatctc gaattcgcag cgttcgccgg cgcagtcggc gaaggcgcag
     3781 cgatcgagct tcgcggcttc ctgaccatgg ttcggagcaa gctgccgaat ccggacagca
     3841 tcattctcga cccggacaat gcgccgattc ccggccggcc cgatttgctg tgggcggtca
     3901 cgaccgcctt ggcgaaacgc gccagcgaca ccaactgcgg ccggattttc cggtacgcag
     3961 agcggctgtt tgacgacggg aagtccgagt ttgccgtgat gatggtgaag gacatcaacc
     4021 gcctcacgcc atcggtacgc gacacgcagg actggacgcg tctggtgctc acgaacctcg
     4081 gcaagtcttt ccttgcctga ttgaccatta ttcaaccctg cgggacttct gaccccgcaa
     4141 gggtcagggg gcccgcgatt ctatcgagga gtcgcacatg atccccaatc tctctaagaa
     4201 agcgatgctg gtcggcctcg ccattcgcgg ctggcaggcc aggaaatacg accggaagat
     4261 cagcatcgag gttgccgaac agcatgccgc cacgcccgat gcggggcgct tcaacaagca
     4321 cctgcttccg ggcaatgccg aatcgtacga ggccgttcac aagaaggggc gcgaactgcg
     4381 cgccttctac tacgagaaca ccctgccatg gtcgaaagaa gggcagagga tccttccggc
     4441 ggccaacttt gaggacttcg ccgaaggggt ccggcggttc aagcgcgagt acgcgatgct
     4501 ggtcgaggag ttcctgcggg agtatccgat cctgaaggaa gatgcacgtg tcctgttgaa
     4561 cggcatgttc aacgaaatgg attatccggc ggtcgaggac atgcgcgccc gattctcgat
     4621 agagctcgaa acgctccctt taccggccgc cgaggacttc cgcgttgcgc tggccgatga
     4681 ggaggtggcg cggatccagc gcgacatcga gaaacggctg cagcatgagt tcgcgaacgc
     4741 caatcaggac ctttggaacc ggctgcgcca tgcggtcgac aacatggtgg ttcggctcag
     4801 caacccggag ggccgcttcc atgacacgct ggtgagcaat ctccagtcga tcgtcgagct
     4861 gatcccgaag ctcaatttca cgggcgaccg caatctcgag cagatcggcg cgacgtgcgc
     4921 cgaagcgctt gcggtgcatg acccgcaggc attgcgggac gacgcgattc tgcgtgccag
     4981 ggtcgccgcc caggccaagg agatctcttc catcatggac gcttacatga gttgagcggc
     5041 acccgaaacc gccgccccgt gcgcggggcg gcattctcag gaggaaattt catgaaggcg
     5101 aaagagtatg tgtctgcggc caggaggaat ctggtgctgg atcatccctt cttcggggtc
     5161 ctctccctga agctggatct gatcgaggat cgctcagtca agactctccg tacggatgga
     5221 cgcaagctct ggttcaaccc ggagtacgca tccagcctga accgctacga gattgtcggg
     5281 gtcgttgccc acgaggtgct tcactgcgcc aatggtcacg cctggcgccg cggtgaccgt
     5341 gatcccaagg tgtggaatca ggcgtgcgac ctcgcgatca atccgatcgt gacaggggcg
     5401 ggtttggtgc tgcctgaggg ggcgctggat ggttctgcgt acaaggggat gagcgcggaa
     5461 gagatctacg gaaggctggt gcagcaagcg caggatcagc aacgggaaga agaggactca
     5521 tcggacggcg gaggcggcca gcaaggcggt caatccgccc aaggtgacga ttcgggtgac
     5581 gatacccagg acggtcaaga tggtggtggc gaagcgccac gacctgacgg atcggagtgt
     5641 ggcgaagtta ccgactgcga agccgatcag accccggaga ttcaggcgga ctggtcagcg
     5701 gcggtcctga atgctgccaa acaggccgaa gcgatgggca ctttgccggc agggatgcag
     5761 cgcctcgtcg aggaaatcaa gaatcctcca cgagattggc gctcgatctt gcgccgcttc
     5821 gtccagacga gcgcaagcca tgactactca tggcggcagc cgaacagccg gtacttgtac
     5881 gccggtctgt acatgccggc tcttcgcgcg gagagcatgc cacccatggt gatcgcggtg
     5941 gacacgtccg gcagtatcga cgacctgacg gtcagccagt tcgccaagga gatcaacgcc
     6001 attgcggacg aagtgcagcc cgagcaggtg cacgtcgtct actgcgacgc ggagatccac
     6061 ggggtggacg ttttcgagcg tggcgaaccg atcgctatca accccaaggg gtacggtggc
     6121 acggatttcc gcccggtctt cgaatgggtc gccaatgaag ggctaaaccc gtcctgcctt
     6181 gtctatctaa ccgacatggc agggtatttc cccgagtcgg cgccggatta tccggtgctg
     6241 tggggagata cctctgggat gttggccgcc ccatggggcg agaccgttcg aatccgctgc
     6301 aactgaaaga aattcctccc gtcgcaaggc gggaggggtt tcccaaagcg cattgaacag
     6361 cgtgctttgg gaaaccaggg tccagcttgg tggttacgcc atcgagcaga agaaggtagc
     6421 tggcctttcg aaacaaacag catttgagcg gcgcggggtg acccgatgcc ttgacaaccc
     6481 cgccggggac gcatgttccc cggcggggga cgtgtgcctc cagcggattt ctctttggag
     6541 gagcacgtgg acaaagaaac gatcaagaac ttcattcact ggctcgacca ggccggcgac
     6601 gaggaactcg aacggaatcg ccagtacatc ttggcgaaga tgaagctggt atcgacgagc
     6661 gaaggccggg ccgtgttcaa gctcgcgctt cgtctgctcg atgaggaagt gctgtgcggg
     6721 ccgagccctg acatcgtcag gctggtaagg cagcgcgacg cgctcctggc cgccctcaac
     6781 cacatcgagg tagacaagga cggcgacggg ttcatctgtc gcgaggcgat ggacgaggtc
     6841 cgccaggcga ttgctctggc aacggaagtc gcagcacagg cagtacgcac accggccgtg
     6901 tgattcgcgc ggctgcgtca ttcatcccga accggggcat cgttccccgg ttggggagcg
     6961 gtgccccttt ttgcttttga ggaggcactc atgaatctgg aatcaatgga caacaccgat
     7021 ctgctggcga tgctggtggg ccggaggttg gcccgatcat tgtcgggcag gccgctcgcc
     7081 gagatcttcg gctactgccg tccgcggcaa cccgcgctgg gcgagcagcc cgtgccctat
     7141 gtcgcccacc cagccatcgg ggcggcgaaa gagctgttca cgcgctgcat cctggaacgg
     7201 atggcggtgg aggacctttg cttttcctcg ccggaaaccg tccaggcgtt cctgtgctcg
     7261 aggatcggtc atctggaaca tgaggtgttc tggagcttct ggctggacgc gcaaaaccgc
     7321 ctgatcgtgg ccgagcagct attccaaggc acgcttacgc agaccagcgt ccatcccagg
     7381 gaagtcgtga agaaggcgct tgcccgcaac gccgcggcgg tgatcttcgc ccacaaccat
     7441 ccatccggcg tgcccgaacc gtcgcgcgcc gacgagatgc tgaccaccgc cctcaagtcc
     7501 gcgcttgcgc tggtggacgt cagggtgctg gatcacttca tcgcggcggg caaccaggcc
     7561 atgtcgtttg ccgcgcgggg attgctgtga tgagcgcctt caaacccatc cggcaggaac
     7621 tggctctggt catcgacctc gacgagcgag gctggttcaa ggcacacgtc ggggaccaga
     7681 acggcaagac ggtctttcag ttctcgaacg aggacgagga gaccggatgg ccgggcgagg
     7741 ctggcctatg gctcgtcgaa gacggattca tgcggcacgg acgcgatacc gacggactgc
     7801 tgacgtatct gaagtcgatc ggaatcgcca agccgacggc gaccatgagc ctttgcaact
     7861 actaacccgc cgcacggcgg attccccacc gggatcaccg tcccgctggg gcggctcccg
     7921 atgggtattt ttcccggaag gagatcgttc atgaagaaac tcatcatcac gctcggccag
     7981 gacctgtcgg cgtacgccac catcgaggtg ccggcggaaa ccgatctgtc cgacgagagt
     8041 ctcgccgcga tcgcccgcaa ggcggtcgag gacgagggga ccgtgttcga cgaagactgg
     8101 tcgacagtga atgcccttcg catcgtgctt gtgcaagacg aaaagggcca gaccatccga
     8161 tcggacatac ccgtcgaatc gtcctgctat gagggtggcg cagcactcca gttgtttctg
     8221 aagggcggga tcggcgtcga ggcactcgtc aattcggcga tgaaggccaa gttgatcgac
     8281 ccggcccgaa acggcgcaga agtcgcagca atcgctgctc aggccgatct gttggcggca
     8341 gcggaagccg cgcttgctcg cctcaaagaa tggtccgact tcgcgccgga aatcggcctg
     8401 ctcgaagcgg ccatctccag agtgaagggg cgctcgtcat gacgcccctc agcttggaaa
     8461 accccgttta cgtcgagcgg acgatgtcgc agtggtgtca atgcctgccc agaaggatgg
     8521 gcgtcatcga gttctccgga accggtgacc ccgccttctg cggcagcgcc aacgaccggc
     8581 ctctcggtat cgatggcttc attctggaac ggggctccaa tgcgcctcgc gccgagttcg
     8641 agaccatcga gcaagtccac gaggcggaca acgccatcaa gaaccggcgg cccaactcca
     8701 tcctcggcgt cctgccgtcg tggcgttggg tcccggtagc agtctcacca ccccgcgttc
     8761 gcgcggggct ttcccgagcc gattccatgc gtcggctgcg gaaagccgga ttctttcgat
     8821 cgggtaaaca cactcatccc ttcaagcgag gctctggcca gcgcagtatg catcgcggat
     8881 gcgccagcgc tgatgccggc ggccatcggg tcttcggtca ggcggtggcg agtgtcagat
     8941 gcccgatcgg cccggccgcc ggacgcacct tgatttcgat gtcgtagccg aggcggttca
     9001 ggcaatccat cagcttgcgt tcggacaggt tggtgaagtc gccgcgcatc atggccgaca
     9061 ccttcggttg cgggatgccc atgcgcttgg ccgccgcctg ttgcgtcagg ccgagggcac
     9121 gcatcgcctt cctgatttcg gccaccaagc cggtcttgat cttcagtttc tcggcgtcgg
     9181 gcagtccaag atcggcgaag acgttgcccg agctgcgttg aacctcgaca ccttcaaaga
     9241 ttcgtttttg catttcgtag ctcctgtgcc attgcctcgg ccaccctcag ccgagcgcgg
     9301 atgatgtcca tgtctgtctt cggcgtggcg atcccgctct tgctcttctt ctggaagcaa
     9361 tgcaggacga acaccgcttc cgcaaacttc actgtgtaga cagcccggta cgtgccgcca
     9421 gcatcgtctt cgacgacctc cagcacgccg gccccaccga aacccttgag caccttcgcc
     9481 gcgtcgtcct ggtcgcccat ctgggccagc gacaacgcgt aaccgaaacg tcggcgcacg
     9541 tcgggcggca acgccatcag atccttgtag ctgctcgcaa tccattcgag cggcttttct
     9601 ttgtcggcca tgtcggcaga ttatacctgt tcaggtaaga ccgcaagtgc ggcagactca
     9661 atctgcgttt gacaaaaaac aaatggttgc gaataccacc tgcaacagtc ctgtgcctcg
     9721 atggttatgg gcatttcagc atgccgtatt catgataacg gccattatca agaacgccat
     9781 ttttcgccga caagttgggt gacggagaat cgctgtgctg gaatgtcaaa caaatcatga
     9841 cgttgtacga gctttcaggc acacccaggc tgttgtcgcc gttacgatca gacagggcac
     9901 ctggttgctc gggggaaggc actggtctgg tctccatcaa gtgcacagac gatgcacaga
     9961 cgatgcactg acggtacgct cggtggcctg gcaccggcgg catgccgctt tgaaggcaca
    10021 ggcagtgcac tgacaggcga caccggcccg aaggccgacc ccgcgcgtcc gcattgggtg
    10081 cgctgacggt gcactggcgg gacggcgtct cgccatttgc tattcgcaaa tagcgaatgg
    10141 tcagcagccc gcacgcggca ctattcctcc agcctgcgat agccgtgccg gacacgcagc
    10201 cgctcgatcc gcttgagttc gcgtagggcc gcgtcctcct ggaggaaaac ttcgcgcttc
    10261 acgccgccgc gcctattgcc gagaccatac cagcgccgga cgagtacgaa ggcgccgaac
    10321 aagtcgcgtt cgagggcgag gatgtagcca cgggtatcgc tcctgaactc aatccgcatc
    10381 ggctgcctcc aggttggcca actcctgcca cactcgctct tcatccgatt ccgtatagat
    10441 ggaggtggtc gccaggctcg cgtggcccag gagcttctgg atgaggttgg ggggaacccc
    10501 ggcaaggccg aggtggttgc cccgggtgtg gcggagccag tgcacggtgg cccgatcgaa
    10561 gtccttggcc tcctgcatgt gaccctctgc ccggagcgcg tcggccgcgc cctggaatag
    10621 agccctcaac gccttgtaga ggctgctggg ggatacgggc tcgttgccgt cgagtcgggc
    10681 gatcagcggg gtctcggacg gattcgccaa gggatcggga gcaaggccgc gataggtcag
    10741 gtattctccc aaggcgatca ggacgccttc cgagatcggc acggcccgcc atttccctcc
    10801 cttgccgcgc accttgagca tccatcggac acccaacgag tccctgagcg gcatggtgta
    10861 gagccgcccg atcttcgcat ccaccagttc ggacaaccgc agaccagtgg cataagcgaa
    10921 cgggagcacg aagcgcagtc tgtgggagtg tgcatcgcgc ggcagctggt ccaagtggtg
    10981 ggtcaagtat cgccactgtg cctgggagaa cacccgggtc agctcgatgt cggccggtgc
    11041 gtcttcttgg gtcgtcagct ttttgccaac tgcgtccaaa gggttgaagg cgcagtattg
    11101 gacgcgcacc agccactcga acaggccgga aacgatggtc agggcctggc gcacgcttga
    11161 ggccgagagg gggccgtcaa acgggcgcca ggcggggctg aagcgcggcg tattgcgctt
    11221 gccgatccag tcgctctgag ggactcggaa aacccagtgc tcaggagagc ttcgcccgag
    11281 cgccgacagc cagtcgcggt aggccgcgca atcgtcgacg gtgagatcgg agagcgcctt
    11341 ttggcgctcg atcacggccc acagcagcag gcgctccgat tccttgcggt atgcgcgctc
    11401 ggtattggag ctgcccgatt tcgtcgcaag ccatgactgg atggcttgaa gatcgttgac
    11461 cgcctgaatg cgcggctggc cgggatagcg attggaaccg gtcgcgccgg cgagcgcctc
    11521 ggggacgacg aatgcctcca tcggcacaat ggcggtttcc cgattcctct cccggatgag
    11581 cgccggcaca ggctgggtgc gaacgggcgc gagggcatgg ccgggcaggg cgcccaggga
    11641 cgactcgtag ccgcgcagcc aagcgacgat ccggttggcg cctttctcgc ccagcttcgg
    11701 taccgtgacc caccagcggt atcctcgtcc ccggatgcgc tccagaagag ccccgacggt
    11761 cgggatgccg gcaagaatga ggcggtccgc gatgggctgg tcgaaccagg cggacaccag
    11821 atcggccggc acggggtcgg tgaccagcag tcgttcgatc cagaccaggg cggcgagctg
    11881 gcggtcgatc agccgctggc gctgccgacc ccttttgtcc cgcacttccg gaaaagcatc
    11941 gaggtagagc tggatcaact cctcctctcg atagaaaccg cctggatcgt gttcagcccg
    12001 gaaatcgtcc agcgacgggc agggaagggc tttggcttgc tggccggggg ccagatggag
    12061 ccgcagcaac cgtgcctctc cccggtggcc gtggcgcagg gcggcctgac tcagcgtgtc
    12121 ccgcagccag acaagggtgg ccttcgcgag ccgaagatcc agtccggtct cgaggtagcg
    12181 atccgccagg gccttgagat cgagcccctg gagccagccg cggtagagcg ccaggtgatg
    12241 gcgaccgact ttccgttcaa ccggaggcat gcgtcatacg ccgcagaagc gattcagcct
    12301 ctcgcgccac ttgcgcagca cggcgcaacc aggatgccat ttggcggaag cctggccggc
    12361 ctggtcatac cccgtccccc aacgacttgg cgaacgtcag ggcgcgattc gaagccccat
    12421 ggctctgcct ggctgccggc gcgtgtgggg gtgtggctgc tgtggcaggg aggatcccac
    12481 cagacacggc ggccaggccc agcgtggcca gcgtgcggac gcgctcggcc cgaagcctgg
    12541 cgggcacgtc cttcagcccg gcgaaaagct ccgggctcgc cggcgtgacc gtgagcacga
    12601 cgcggatgct gctcacaagg cagcccccat caaccagaac ccgcgggcat tggagaacac
    12661 cgattccttg ggcgtgagca cggccagccg cggaaaggcg tcttgcacgg cttcacggaa
    12721 gaaggcggcg ccgccgccga cgagaaccac gaggtcaggc atcttgcttt ccgtccgcag
    12781 cgatttctgg atcgattcga tcacgacggg gccgacggtc ttcgccgcct gctcgatgta
    12841 gggtgcgacc tccacgcgct ggccgagaac gagcacggtc gccttgccgg ctcgaagggc
    12901 gttttccagg gtttcgacgt tgagcgccga tccgtgatcc ttggcgatca gccgggacgc
    12961 ctcctccagg accacggaag aagcgttgag gctcgtgccg gaggactgcc ggtgcagatc
    13021 cttgtgggcg atcacgaccc agtcgaccga gaagaaaccg ggatcgacca ccagcacccg
    13081 tgcatcgtcg atatcggcgt cctcctgggc gatgtagtcg aggagcccgc cgatcggctg
    13141 agggatgacc ttcacccttt ccacggagac ggtccgcttg ggcgtgactt ggtgggttcc
    13201 ctgcatttgc gcagcgaggg cgttgcgccg cgtctcgtcc aggtattgcg agaccggcag
    13261 cccggtgacg agcgtgtcga tccgatccat ctcggacagg agcaggccgg catggaacag
    13321 cgccgcgtag gaattgctgg atgcgtagtc cgcatggagg gagcggctcc aaagctcggc
    13381 gcggtccggc gagacgccgg cgacgaacgc ctgcccgttc acgagcacgt gcaggaagtc
    13441 gtcctgcgcc ttgccgtcga agcgggagcc gaaccggtcg gccggggctg caccggcggg
    13501 gcgcagatgg gtttttggtg ttccaccctt ctgcccgaag gcgagtttca agttggaata
    13561 cccgatgtca atgccaagga tgttcaaatc gacctcctgt gaatcgtgag agaacaagct
    13621 gt
//
LOCUS       DAOQPA010000096        13374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2385_length_13374_cov_16.833020, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000096 DAOQPA010000000
VERSION     DAOQPA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_13374_cov_16.833020"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..2176)
                     /locus_tag="PKX01_12785"
     CDS             complement(128..2176)
                     /locus_tag="PKX01_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HMZ77051.1"
                     /translation="MTFRRSELTAACLIACLLLAGCGGSDSPASAATATPIAPKGTTA
                     TLALLETTDLHTNVMSYDYFKLAEDQSLGFERTATLINAARKEFPNTLLLDNGDTIQG
                     TALSDYQALVSPIACDQTLAIYKVMNAAGFEGGGIGNHEFNYGLPYLSQVTGNRFNVT
                     GMADPSAQPVCKGPSFPQVLANVFSVKTEQPLFQPYTIINKVVTGTGPDGKSVSGTVK
                     VGIIGFTPPTILSWDKRWLDGKVYTLGVKEAAEKYIPEMRAKGADLVVVISHGGLDNS
                     TYSPTMENGSYHLSKVAGVDAMLIGHSHQIFPNATSTVAQFNLPGVDKTAGTVNGVPT
                     VMANYWGKHLGVIKFELAFDGSTWTLDKTKTVVQARSTQNADKTYVAVDATVGAAIAA
                     EHAATIAYVKTPIGSTDYEMNSYFADVGDPGAIELVNQAQADYVAKYVAANLPQYASL
                     PVLSVSAPFKSGFAGGTDYTDVAAGNMAINNAADLYLYPNTVYAVKVTGADIKNWLET
                     AAKRFNQINPALTTQQELVSSYPGYNFDMFTTADIQYEIDVTQAVGSRIKNLSYKGTA
                     IDPAQSFIVATNNYRASGGGSFPGLDGSKTIYASPDANRDVLIAYIKAAKTITRAANG
                     AHKSWRFTKVSTAGPVVLHSAQGKLALAQAAGLGGVSLLQADDGSGKGLALYGIDLSK
                     "
     gene            2411..3283
                     /locus_tag="PKX01_12790"
     CDS             2411..3283
                     /locus_tag="PKX01_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMZ77052.1"
                     /translation="MGLRLKFNLVLLAVFMAGFGVAGYISHGLLDRHAREQVLGEARL
                     VMEAALAVRGYTVAQVRPHLSAQMDKQFLPQTVPAFAATETLNELRKKYPNYAYKEAT
                     LNPTNPRDRAADWEADLVNTFRNTADSKEIAGERDTPTGRALYIAHPIRIESPACLAC
                     HSTPEAAPASMIKVYGTANGFGWKLNETVGAQVVSVPMSVPLENAAQAFRTFMTSLAA
                     VFAAVFVVLNLMLSWLIIRPVSQMSDAADKISTGDFDQPEFPEQGTDEVAVLASSFNR
                     MRRSLEKAMQMIDG"
     gene            3291..5345
                     /locus_tag="PKX01_12795"
     CDS             3291..5345
                     /locus_tag="PKX01_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ77053.1"
                     /translation="MSDTPDASAAPPPRDDALILPPGYEINEYRIERVLGGGGFGLTY
                     LARDTHLDCRVAIKEYLPRELAGRVAGRAVRPHSETHAPRFERGLQNFLQESRALASF
                     HHRGIVRVLRYFRANDTAYMVMEYETGQPLHKWLVGRLPLDKATLVRIIRPLLEGLEA
                     IHDAGFLHRDIKPGNIYIRADGSPVLLDFGSARRLQSDGADQTLTTVVTPGFAPAEQY
                     HRHGKQGPWTDLYALAGVMYWMVTGRQPIEAFARLKEDIMPSASSLASADIYGGALLR
                     AIDWALDPDENRRPRKVADFRRVCLAPPTGGEGSDAPVEAPLPFGLGSSEARTFIGAV
                     LFADVGEARPGEEDEHKARRSKLATLLGQTVAPQPATTRLAINTREGCAVCYVGDPED
                     ALRTAVQLGDAAAGQGLPLHMGINLGPVRVEPDPKGRSRILGDGITTAFRVMRFAEPG
                     QVLVARTYFDVIQHLKRNAGACFRHLGLRRDPQARDHDVFLYTGEPLPGTVHVAEASR
                     PTQAMGADITPEVVDAAERLLAQHIGPMARILVRKTVPRAASRDELHRLLAALIPDPA
                     PQAAFLSGVLLASGAPSLSAHPATGPVTRGTAAVSAGSAPASSATIAAPISSVIDEAD
                     LDRVGKLFARHIGPMAKVLLRREARQANSLETLCRALASHIDKDAERQRFLTDAGFGG
                     LG"
     gene            5391..8108
                     /locus_tag="PKX01_12800"
     CDS             5391..8108
                     /locus_tag="PKX01_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ77054.1"
                     /translation="MSEHESPPAAPSRRNWYWTVPYIAVLVFALAMLVLVWILQRQAA
                     DTQRNALARDVQWAEQTMRLHMQGTEEFLAQLAREKAAGTLDGDGFQVRANQHIANNA
                     ELVNVVWLDADERVRWTAPFDTTDWLEGDALSPVQAQTFHRIRDQGRPAYGDPYVNAR
                     GMAVLEFYVPVLQGRTVVGAIVAVYSIERMVSHLVPSWFAEKYRLAFTGPRGESMAMN
                     SAAHPLDENLSFSVPVDPPGNGLTLNATAYSTESALPRGLPMAVIGVLSFFVLWSLWL
                     LRAHMLRRVRVEKERDRIFNLSLDMLAIIGLDGTFRRCNPAFRAILGYAPEDLPGRPL
                     LDFLHAEDVAATVRELRNLASGVPGTFENRCRCADGRYKWLVWSVNSVPEEKLMYAVA
                     HDITGRKSAEEALRAEYAFRKAMEESLVTGLRAIDLSGRIIYVNPAFCRMTGWSREEL
                     VGAVAPFPYWPREEYATLRGNLELTLAGNAPPTGFEMRIQRKNGERLDARFYLSPLID
                     GDGRQTGWMASITDITEPKRVRAELEAAHERLVAVMDGLDAAVYVADAADDEILFANR
                     AFKSIYGFDAVGRPVGAFGLPTPPDTQWYDVDPRRLAPRDVPRELYDGELRHGLSGRW
                     YHIRERAIRWVDGRVVRMAIATDVTDRKRLDEENRVQQERLQRTSRLITMGEMASTLA
                     HELNQPLAAIANYAMGCVNRLQSGEYRQEDILAAMQKASAQAERAGRIIRRVREFVKK
                     SEPRRSPVALAGIVEDAIGFVEVDARRHAAQVVSEIPADLPPVYADAVMIEQVVLNLL
                     KNGIEAMRDTPPEARIVTLGAVALPDGLVEVSVADRGHGLGDDALDKLFSPFYTTKSE
                     GMGMGLNICRSIVEFHQGRLWVDPNPAGGCIFRFTLPLETARESVRDAA"
     gene            8098..8727
                     /locus_tag="PKX01_12805"
     CDS             8098..8727
                     /locus_tag="PKX01_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ77055.1"
                     /translation="MPPEPAAEHIVYLVDDDEALRDSLVWLLESQGFQVAAYASAEDF
                     LANWQPDLSGCLLLDVRMPGMNGLELHERLKARFSTLPVIFITGHGDVPMAVSALKGG
                     AADFIEKPFNDADLLRLVSQCLSREREEGARRRQGAEVSRRLAQLTVREREVLDLIIA
                     GKLNKQIADTLGISIKTVEVHRARVMEKMGAQSLAELVQAVLGVEAGVR"
     gene            8844..9713
                     /gene="folD"
                     /locus_tag="PKX01_12810"
     CDS             8844..9713
                     /gene="folD"
                     /locus_tag="PKX01_12810"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HMZ77056.1"
                     /translation="MTARILDGNALSAKLRGELAARAATLTAQGVQPCLAVILVGGDP
                     ASAVYVRNKVAGCEKAGIKSLKYEYPQDAAPADVLGRIAALNADPAVHGILVQLPLPP
                     QFDEKAVLEAISVEKDVDGFHAENVGRLSQGQDAFVPCTPNGVMEMLKSENVPLRGAE
                     AVVIGRSNIVGKPMAMLLTAAGATVTVCHSATRDLAFHTRRADILVAAVGRPRFVTGD
                     MIKPGAVVIDVGINRLTDGPDAGKLCGDVDFASAAPVAGLITPVPGGVGPMTITMLLA
                     NTVQSAGRVARKG"
     gene            9717..10214
                     /gene="purE"
                     /locus_tag="PKX01_12815"
     CDS             9717..10214
                     /gene="purE"
                     /locus_tag="PKX01_12815"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMZ77057.1"
                     /translation="MNAANPVVGIVMGSNSDWPTMQAAAKVLRDFGVPFEARVVSAHR
                     TPDLMFEYAEQARARGLQAIIAGAGGAAHLPGMLAAKTTVPVLGVPVQSKALSGKDSL
                     LSIVQMPKGIPVATFAIGEAGAANAGLFAVALLAGGDAALAARLEAFRAEQTASVLAM
                     TLDPI"
     gene            10231..11385
                     /locus_tag="PKX01_12820"
     CDS             10231..11385
                     /locus_tag="PKX01_12820"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMZ77058.1"
                     /translation="MILPPATLGMLGGGQLGRFFVAAAHEMGYKVWVLDPDPHSPAGL
                     IADRHLQAGYDDFAALDALAAGCAAVTTEFENVPADTLDYLAKFVPVRPGAAAVAVCQ
                     NRIVEKTFLQDNGLPHGPFVPVRSEADLAAAPAELFPAVLKVARFGYDGKGQARVANV
                     AEAVHAFHAFKGEACVLEALLTLDCEVSVVLARGEDGTVSAFPVVENAHSRGILDVSI
                     APARVAPALADEARRCASLIAERLGFIGTLAVEFFVSGGRLVVNEMAPRPHNSGHHTI
                     DACVVSQYEQQVRALCGLPLGEARQHSASVMVNLLGELWYEGLDPHGRYREPDWSVLH
                     AVPGLRLHLYGKHHARHGRKMGHFTVIGDDPAQVLDAALAARRAIGIRDI"
     gene            11398..12411
                     /locus_tag="PKX01_12825"
     CDS             11398..12411
                     /locus_tag="PKX01_12825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ77059.1"
                     /translation="MHDQAADLDRAVRLLAAGDLVGIPTETVYGLAADARNPAAVARI
                     FAAKGRPADHPLIVHLADAAQLDAWARDIPDAARRLAAACWPGPLTLILKRQPDVPDA
                     VTGGQDTVGLRIPAHPLTLALLRAFAAAGGSGAVAAPSANRFGRISPTIAEHVHQELG
                     DRVAQVLDGGPCAVGLESTILDLSRDVPVILRPGAIGADDIARVIGLRPWQRGEAPAA
                     PDPAVSPAPRVSGALAAHYAPRTPLELVPADRLTEQAEAGDAVLARRPSPAGLRPAVT
                     WIEAPADATAYGHDLYARLRALDASGCARILVEAPPDTPAWAAVADRLGRAAVGSGAP
                     EAT"
     gene            complement(12631..12939)
                     /locus_tag="PKX01_12830"
     CDS             complement(12631..12939)
                     /locus_tag="PKX01_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77060.1"
                     /translation="MKINLIPLGARFEYEGEIYTKTGPITAASEKSGQRMIPRYAVLR
                     PVGELPPAPPPAAERQIAVDTVCAAFEVYHAVALRHVDDFARPALEAARQHFLAALTR
                     "
     gene            13298..13373
                     /locus_tag="PKX01_12835"
     tRNA            13298..13373
                     /locus_tag="PKX01_12835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13331..13333,aa:Ala,seq:cgc)
BASE COUNT         1927 a         4683 c         4656 g         2108 t
ORIGIN      
        1 ggcgtcggcg aggagctgga gctggagcgg gtcgtcgggc agggccgggc ggtgttcgcg
       61 gatcgtccag gcgaggccgg cggccaccgc cgccccggcg atcagggcgg cggcgacgcg
      121 gccggactta cttggagaga tcgatgccat acagcgcgag gcccttgccg ctgccgtcgt
      181 cagcctggag caggctcacg ccgccgaggc cggcggcctg ggcgagggcg agcttgccct
      241 gggcggagtg gagcaccacg gggccggccg tgctcacctt ggtgaagcgc cagctcttgt
      301 gggcgccgtt ggcggcgcgg gtgatggtct tggcggcctt gatgtaggcg atcagcacgt
      361 cccggttggc gtcgggcgag gcgtagatgg ttttgctgcc gtccaggccg gggaagctgc
      421 cgccgccgct ggcgcggtag ttgttggtgg cgacgatgaa ggactgggcc gggtcgatgg
      481 ccgtgccctt gtagctgagg ttcttgatgc ggctgccgac ggcctgggtg acgtcgatct
      541 cgtactggat gtcggccgtg gtgaacatgt cgaagttgta gccggggtag gaactgacga
      601 gttcctgctg ggtggtgagg gcggggttga tctggttgaa gcgcttggcg gcggtttcga
      661 gccagttctt gatgtcggcg ccagtgacct tcaccgcata gacggtgttg gggtagaggt
      721 agaggtcggc ggcgttgttg atggccatgt tgccggccgc cacgtcggtg tagtcggtgc
      781 cgccggcgaa gccgctcttg aacggggcgc tgacggagag cacgggcagg ctggcgtact
      841 ggggcaggtt ggcggccacg tacttggcca cgtagtcggc ctgggcctgg ttgaccagct
      901 cgatggcgcc cggatcgccc acgtcggcga agtagctgtt catctcgtaa tccgtgctgc
      961 cgatgggggt cttgacgtag gcgatggtgg cggcgtgctc ggcggcgatg gcggcgccca
     1021 cggtggcatc gacggccacg taggtcttgt cggcgttctg ggtggagcgg gcctggacga
     1081 cggtcttggt cttgtcgagg gtccaggtgc tgccgtcaaa ggcgagttcg aacttgatca
     1141 cgcccagatg cttgccccag tagttggcca tcacggtggg cacgccgttc acggtgccgg
     1201 cggtcttgtc cacgccgggc aggttgaact gggcgacggt gctggtggcg ttggggaaga
     1261 tctggtggga gtggccgatc agcatggcgt ccacgccggc gaccttggag aggtggtagc
     1321 tgccgttctc catggtgggc gagtaggtac tgttgtcgag gccgccgtgg gagatcacca
     1381 ccaccaggtc ggcgcccttg gcgcgcattt cggggatgta cttttcggcg gcttccttga
     1441 cgcccagggt gtagaccttg ccgtcgagcc agcgcttgtc ccagctcagg atggtgggcg
     1501 gggtgaagcc gatgatgccg accttgacgg tgccgctgac gctcttgccg tccgggccgg
     1561 tgccggtgac gaccttgttg atgatcgtgt agggctggaa caggggctgc tcggtcttca
     1621 cgctgaagac gttggcgagc acctgcggga agctcgggcc cttgcacacg ggctgggcgg
     1681 acgggtcggc catgccggtc acgttgaagc ggttgccggt gacctggctg aggtagggca
     1741 ggccgtagtt gaattcgtgg ttgccgatgc cgccgccttc aaagccagcg gcgttcatga
     1801 ccttgtagat ggccagggtc tggtcgcagg cgatggggct gaccagcgcc tggtagtccg
     1861 acagggcggt gccctggatc gtgtcaccgt tgtccagcag cagggtgttg gggaattcct
     1921 tgcgggcggc gttgatgagc gtggcggtgc gctcgaagcc gagggactgg tcctcggcga
     1981 gcttgaagta gtcgtaactc atcacgttgg tgtggaggtc ggtggtctcc agcagcgcca
     2041 gcgtggcggt ggtgcccttg ggggcgatgg gcgtcgccgt ggcggcggac gcgggggaat
     2101 cgctgccgcc gcagccggcc agcagcaggc aggcgatcag gcaggcagcg gtgagttcgg
     2161 aacgacggaa ggtcatggca cgcacctggg aggggaaagg ttcctcccag cttcgcgcgg
     2221 cgacattgcc tgtcagtgaa cggcacctta taaattgaag acggcagggt gaagttgcgg
     2281 tgacggcgcc cgcccatggt ggatcgccct gcggctgccc gcgaatgcga atggggcgca
     2341 atacgcggcc cgcctgtgcc agaatgtcac ccggtttgcc ggacgaccgg cgattgagga
     2401 gggtatagcg atgggtctcc ggcttaaatt caacctggtc ttgctggccg tcttcatggc
     2461 gggctttggc gtggcgggtt acatctccca tggcctgctc gaccgccatg cccgcgagca
     2521 ggtgctcggc gaagcgcgcc tggtgatgga ggccgccctg gcggtgcggg gctacacggt
     2581 ggcccaggtg cgcccgcacc tgtcggcgca gatggacaag cagttcctgc cccagaccgt
     2641 gccggccttt gcggccaccg aaaccctcaa cgagctgcgc aagaaatatc ccaactacgc
     2701 ctacaaggaa gccaccctca accccaccaa cccccgcgac cgggcggcgg actgggaggc
     2761 ggacctggtg aataccttcc gtaatacggc cgacagcaag gagattgccg gcgagcgcga
     2821 cacccccacc ggccgcgccc tctacatcgc ccacccgatc cgcatcgagt cgccggcctg
     2881 tctggcctgt cacagcacgc cagaggcggc gcccgcctcc atgatcaagg tgtatggcac
     2941 cgccaacggc tttggctgga agctcaacga aacggtgggc gcccaggtgg tgtcggtgcc
     3001 catgtcggtg ccgctggaga acgcggccca ggccttccgc accttcatga cctccctggc
     3061 ggccgtgttc gcggcagtct tcgtggtgct caacctgatg ctgtcgtggc tcatcatccg
     3121 ccccgtgtcg cagatgtccg acgccgccga caagatcagc accggcgact tcgatcagcc
     3181 ggagttcccc gagcagggca ccgacgaagt ggccgtgctg gccagttcct tcaaccgcat
     3241 gcgccgcagt ctcgagaagg ccatgcagat gatcgacggt tgagcgcttc gtgagcgata
     3301 caccggacgc gtccgccgcc ccaccgcccc gggacgacgc gctgatcctg ccgcccggct
     3361 acgagatcaa cgaataccgg atcgaacggg tgctgggcgg cggcggcttc gggctcacct
     3421 acctggcccg cgacacccac ctggactgcc gggtggcgat caaggagtat ctgccgcggg
     3481 aactcgccgg gcgggtggcc gggcgcgcgg tgcggcccca ttccgagacc cacgccccgc
     3541 gtttcgagcg gggactgcag aacttcctcc aggaatcccg cgccctggcc agcttccatc
     3601 accggggcat cgtgcgggtg ctgcgctact tccgcgccaa cgacacggcc tacatggtca
     3661 tggagtacga gaccggccag cccctccaca agtggctggt gggccgcctg cccctcgaca
     3721 aggcgaccct ggtgcgcatc atccgcccgc tgctggaagg cctcgaggcg atccacgacg
     3781 caggcttcct gcaccgggac atcaaacccg gcaacatcta tatccgcgcc gacggctcgc
     3841 cggtgctcct ggatttcggc tcggcccggc ggctgcagag cgacggcgcg gaccagacgc
     3901 tcaccaccgt cgtcacgccg ggctttgcgc cggccgagca ataccatcgt cacggcaagc
     3961 aggggccgtg gaccgacctg tacgccctgg cgggggtgat gtactggatg gtcaccgggc
     4021 gccagccgat cgaagccttt gcgcgcctga aggaagacat catgccgtcg gcgagctcgc
     4081 tggccagcgc cgacatctac ggcggcgcgc tgctgcgggc catcgactgg gcgctggatc
     4141 ccgacgagaa ccggcggccc cgcaaggtgg ccgacttccg ccgcgtctgt ctggcaccgc
     4201 ccaccggtgg cgaaggctcg gacgccccgg tggaggcgcc gctgcccttc ggcctggggt
     4261 cgtccgaggc gcgtaccttc atcggcgcgg tgctgtttgc cgacgtgggc gaggcgcggc
     4321 cgggcgagga agacgaacac aaggcccgcc gctcgaagct ggccaccctg ctgggccaga
     4381 ccgtggcgcc ccagcccgcc accacgcggc tggccatcaa cacccgcgaa ggctgcgcgg
     4441 tgtgctacgt gggcgacccg gaagacgccc tgcgcaccgc cgtgcaactg ggcgacgccg
     4501 ccgccggcca gggcctgccc ctgcacatgg gcatcaacct gggcccggtg cgggtcgaac
     4561 ccgaccccaa ggggcgctcg cgcattctcg gcgacggcat caccacggcc ttccgcgtca
     4621 tgcgctttgc cgagcccggc caggtgctgg tggcacgcac ctatttcgac gtgatccagc
     4681 acctcaagcg caacgctggc gcatgtttcc gtcacctggg cctgcgccgc gacccccagg
     4741 cgcgggatca cgacgtcttc ctctacaccg gcgagccctt gcccgggacc gtgcatgtgg
     4801 ccgaggcgtc gcgcccgacg caggcgatgg gcgccgacat cacgccggag gtcgtcgatg
     4861 cggccgagcg cctgctcgcc cagcacatcg gcccgatggc gcgcatcctg gtgcgcaaga
     4921 ccgtgccccg cgctgccagc cgggacgaac tgcaccgcct gctcgccgcg ttgattcccg
     4981 atccggcgcc ccaggccgcc ttcctgtccg gcgtgctgct ggcttcgggc gcgccgtcgc
     5041 tctccgccca cccggccacc gggccggtca cgcgggggac ggcggcggtg tccgccgggt
     5101 cggcgccggc ctcctcggca accattgcgg cgccgatctc cagcgtcatc gacgaggcgg
     5161 acctggaccg ggtcggcaag ctcttcgccc gccacatcgg gcccatggcc aaggtgctgc
     5221 tgcgccgcga ggcgcgtcag gccaacagcc tcgaaaccct gtgccgtgcc ctcgccagtc
     5281 acatcgacaa ggacgcggag cggcagcgct tcctgaccga cgccggattc gggggattag
     5341 ggtaagaccc gatgtcgccc gggccgtatc gcaagcacga tgacggttcc atgtccgaac
     5401 acgaatctcc tcccgctgcc ccgtcccgcc gcaactggta ctggacggtg ccctacatcg
     5461 ccgtgctggt gttcgcgctg gccatgctgg tgctggtgtg gatcctccag cgccaggccg
     5521 ccgacaccca gcgcaacgcc ctcgcccgcg acgtgcagtg ggccgagcag accatgcgcc
     5581 tgcacatgca gggcaccgag gagttcctcg cccagctggc ccgcgagaag gccgccggta
     5641 cgctggacgg ggacggcttc caggtgcgcg ccaaccagca catcgccaac aacgccgagc
     5701 tggtgaacgt ggtgtggctg gacgccgacg agcgggtgcg ctggaccgcc cccttcgaca
     5761 ccaccgactg gctggaaggc gacgccctgt cgccggtgca ggcccagacc ttccaccgca
     5821 tccgcgacca gggccgcccc gcctacggcg acccctacgt caacgcccgc ggcatggcgg
     5881 tgctggagtt ctacgtgccg gtgctgcagg gccgtaccgt ggtgggcgcc atcgtcgccg
     5941 tgtattccat cgagcgcatg gtgagccacc tggtgccgag ctggttcgcc gagaaatacc
     6001 ggctcgcctt caccgggccg cggggcgagt ccatggccat gaactccgcc gcccatcccc
     6061 tcgacgagaa cctgtccttc tcggtgccgg tggacccgcc aggcaacggg ctgaccctca
     6121 acgccaccgc ctattccacc gaaagcgcgc tgccccgcgg cctgcccatg gcggtgatcg
     6181 gcgtgctgtc cttcttcgtg ctgtggagcc tgtggctctt gcgcgcccac atgctgcggc
     6241 gggtgcgggt ggagaaggag cgcgaccgga tcttcaacct gtccctcgac atgctcgcca
     6301 tcatcggtct ggacggcacc ttccggcgct gcaatccggc cttccgcgcc atcctcggct
     6361 acgcgccgga ggacctgccg ggccggccgt tgctggattt cctgcacgcc gaagacgtgg
     6421 ccgccacggt gcgcgagctg cgcaacctgg cttccggtgt gcccggcacc ttcgagaacc
     6481 gctgccgctg cgccgacggg cgctacaagt ggctggtgtg gagcgtgaac tcggtgcccg
     6541 aggaaaagct catgtacgcg gtggcccacg acatcaccgg gcgcaagtct gccgaagagg
     6601 ccctgcgcgc ggaatatgcc ttccgtaagg cgatggagga atccctcgtc accggcctgc
     6661 gcgccatcga cctgagcggc cgcatcatct acgtgaaccc ggccttctgc cgcatgaccg
     6721 gctggtcgcg ggaggaactg gtgggggccg tcgcgccgtt cccttactgg ccgcgggagg
     6781 aatacgccac cctgcggggc aacctggagc tcaccctggc cggcaacgcg ccgcccacgg
     6841 gcttcgagat gcgcatccag cgcaagaacg gcgagcgcct cgacgcgcgc ttctacctgt
     6901 cgcccctcat cgacggcgac ggccgccaga ccggctggat ggcctccatc accgacatca
     6961 ccgagcccaa gcgggtgcgt gccgagctgg aggccgccca cgagcgcttg gtggcggtga
     7021 tggacggcct cgatgcggcg gtgtacgtgg ccgacgctgc cgacgacgag atcctcttcg
     7081 ccaaccgcgc cttcaagagc atctacggct tcgacgcggt aggccggccg gtgggcgcct
     7141 tcggcctgcc caccccgccc gacacccagt ggtacgacgt ggacccgcgc cgcctggcgc
     7201 cccgagacgt gccccgcgag ctctacgacg gcgagctgcg ccacggcctg tccgggcgct
     7261 ggtaccacat ccgcgagcgc gccatccgct gggtggacgg ccgcgtggtg cgcatggcca
     7321 tcgccaccga cgttaccgac cgcaagcgcc tcgacgagga gaaccgcgtc cagcaggaac
     7381 gcctgcaacg cacctcgcgg ctcatcacca tgggcgagat ggcgtcgacc ctggcccatg
     7441 aactcaacca gcccctggcg gccattgcca actacgcgat gggctgcgtc aatcgcctgc
     7501 aatccggcga atatcgccag gaagacatcc tcgccgccat gcagaaggcc agcgcccagg
     7561 ccgagcgggc cggccgcatc atccgccggg tgcgcgaatt cgtgaagaag agcgagccgc
     7621 ggcgcagccc ggtggccctg gcgggcatcg tggaggacgc catcggcttc gtcgaggtgg
     7681 acgcccgccg ccacgccgcc caggtggtca gcgagatccc ggccgacctg ccgccggttt
     7741 acgctgacgc ggtgatgatc gagcaggtgg tcctcaatct cctcaaaaac ggcatcgagg
     7801 ccatgcgcga cacgccaccc gaggcgcgga tcgtgaccct cggcgccgtc gcgctgcccg
     7861 acgggctggt ggaggtctcc gttgcggacc ggggccacgg cctcggcgac gatgccctcg
     7921 acaagctgtt ctcgcccttc tacaccacca agagcgaggg catgggcatg ggcctcaaca
     7981 tctgccgctc catcgtcgaa ttccaccagg gccgcctgtg ggtggacccc aacccggccg
     8041 gtggatgtat cttccgtttc accctgcctc tggagactgc ccgtgaatcc gtccgagatg
     8101 ccgcctgagc cggccgccga acacatcgtc tacctcgtgg acgacgacga ggccctgcgc
     8161 gactccctcg tctggctgct ggagtctcag ggcttccagg tggcggccta cgcctcggcc
     8221 gaggacttcc ttgccaactg gcagcccgac ctctccggct gcctgctgct cgacgtgcgc
     8281 atgcccggca tgaacggcct ggagctccac gagcgcctca aggcgcgctt cagcaccctg
     8341 ccggtgatct tcatcaccgg ccacggcgac gtgccgatgg cggtgtcggc cctcaagggc
     8401 ggcgccgcgg atttcatcga gaagcccttc aacgacgccg acctgctgcg cctcgtgagc
     8461 cagtgtctca gccgcgagcg ggaggagggt gcccgccgcc gccagggggc cgaagtgtca
     8521 cggcggctgg cccagctcac ggtgcgtgag cgggaggtgc tggacctgat catcgccggc
     8581 aagctcaaca agcagatcgc cgatacgctg ggtatcagca tcaagacggt ggaagtccat
     8641 cgcgcccggg tcatggagaa aatgggcgcc cagtccctcg ccgagctggt gcaggcggtg
     8701 ctgggcgtcg aggccggcgt gcgctgagag cgtgcggcgg cccgcgaccg ccgtccggtg
     8761 cgggccggca ccctgttgaa aacgctgggg atttcccctt gcgccgtggt acactccgcg
     8821 gtttttcttt gaggggcgca ggcatgacgg ctcgcattct cgatggcaac gcgctttccg
     8881 ccaaactgcg gggcgaactc gccgcccggg cggctacgct caccgcccag ggcgtccagc
     8941 cctgcctggc ggtgatcctg gtgggcggcg atccggcctc cgccgtgtat gtccgcaaca
     9001 aggtggccgg ctgcgaaaag gccggcatca agtccctcaa gtacgaatac ccgcaggatg
     9061 ccgcgccggc cgacgtgctc ggccgcatcg cagcgctcaa cgccgacccg gcggtgcatg
     9121 gcatcctcgt gcagttgccc ctgccgcctc aattcgacga gaaggccgtc cttgaggcga
     9181 tctccgtcga gaaggacgtg gacggcttcc acgccgaaaa cgtgggccgc ctgtcccagg
     9241 gccaggacgc cttcgtgccg tgcacgccca acggcgtgat ggaaatgctc aagagcgaga
     9301 acgtgcccct gcgcggtgcc gaggcggtgg tcatcggccg ctccaacatc gtcggcaagc
     9361 ccatggcgat gctcctcacg gccgccggcg ccaccgtcac cgtgtgccat tcggccaccc
     9421 gcgacctggc cttccacacc cgccgcgccg acatcctggt ggcggcggtg ggcaggccgc
     9481 gcttcgtcac cggcgacatg atcaagcccg gtgcggtggt catcgacgtg ggcatcaacc
     9541 gcctcactga cggcccggat gccggcaagc tgtgcggcga cgtggatttc gcctcggccg
     9601 ccccggtggc cggcctcatc accccggtgc ccggcggcgt cgggcccatg accatcacca
     9661 tgctgctcgc caataccgtc cagtcggccg ggcgcgtcgc gcgaaagggc tgaatcatga
     9721 acgctgccaa tcccgtcgtc ggcatcgtca tgggctccaa ttccgactgg cccaccatgc
     9781 aggccgcagc caaggtgctg cgcgacttcg gcgtgccctt cgaggcccgc gtcgtctctg
     9841 cccaccgcac gcccgacctg atgttcgaat acgccgagca ggcccgcgcc cgcggtctcc
     9901 aggccatcat cgccggcgcc ggcggtgccg cccacctgcc cggcatgctc gccgccaaga
     9961 ccacggtgcc ggtgctgggc gtgccggtgc agtccaaggc cctgtcgggc aaggattccc
    10021 tgctctccat cgtgcagatg cccaagggca ttccggtggc cacctttgcc atcggcgagg
    10081 cgggcgccgc caacgccggc ctctttgcgg tggcgctgct ggccggcggc gatgcggccc
    10141 tggctgcgcg gcttgaagcc ttccgcgccg agcagaccgc cagcgtgctg gccatgaccc
    10201 tcgatccgat ctgattccgg ggccgacgcg atgatcctcc cgcccgccac cctcggcatg
    10261 ctcggcggcg gccagctcgg ccgcttcttc gtggctgcgg cccacgagat gggctacaag
    10321 gtctgggtgc tggacccgga cccccacagc ccggccggcc tcatcgccga ccgccacctg
    10381 caggccggct acgacgactt cgcggccctc gacgctctcg ccgcaggctg tgccgcggtc
    10441 accaccgaat tcgaaaacgt ccccgccgac accctggact acctcgccaa gttcgtgccc
    10501 gtgcggcccg gtgcggcagc ggtggccgtg tgccagaacc gcatcgtcga gaagactttc
    10561 cttcaggaca acggtctgcc ccacggcccc ttcgtgcccg tgcgcagcga ggccgatctg
    10621 gccgccgcgc ccgccgagct gttccccgcg gtcctcaagg tggcccgctt cggctacgac
    10681 ggcaagggtc aggcccgcgt cgccaacgtc gccgaggccg tgcacgcctt ccacgccttc
    10741 aagggcgagg cctgcgtgct ggaggccctg ctgaccctcg actgcgaggt ctcggtggtc
    10801 ctggcgcgtg gtgaggacgg cacggtgagc gcctttccgg tggtggagaa cgcccacagc
    10861 cgtggcatcc tggatgtctc catcgcgccc gcgcgggtcg ccccggccct ggccgacgag
    10921 gcgcgccgct gcgccagcct catcgccgaa cggctcggct tcatcggcac cctggcggtg
    10981 gaattcttcg tcagcggagg ccgcctggtg gtcaacgaga tggcgccgcg gccccacaac
    11041 agcggccatc acacgatcga cgcctgcgtc gtcagccagt acgagcagca ggtgcgcgcc
    11101 ctgtgcggcc tgcctctggg cgaggcgcgc cagcacagcg cgtcggtgat ggtcaatctg
    11161 ctgggcgagc tgtggtacga gggcctcgat ccccacggcc gttaccggga gccggactgg
    11221 tcggtgctgc acgccgtgcc gggcctcagg ctgcacctgt acggcaagca ccacgcccgc
    11281 cacggtcgca agatggggca cttcacggtg atcggcgacg accccgcgca ggtgctcgac
    11341 gctgccctgg cggcccgccg cgccatcggc atccgcgaca tctgagcggc accgtccatg
    11401 cacgaccagg ctgccgatct cgaccgggcc gtgcggctgc tggcggcggg cgatctggtg
    11461 ggcatcccca ccgagaccgt ttacggcctg gccgccgacg cccgcaatcc tgccgcagtg
    11521 gcgcgcatct ttgccgccaa agggcggccg gcagatcatc ccctcatcgt ccacctcgcc
    11581 gacgcggccc agctcgacgc gtgggcgcgg gacatccccg atgccgcccg ccgcctcgcc
    11641 gccgcctgct ggccggggcc cctgaccctc atcctcaagc gccagcccga cgtgccagac
    11701 gccgtcaccg gcgggcagga caccgtgggc ctgcgcatcc ccgcccatcc gctgaccctg
    11761 gcgctgctgc gtgcctttgc cgcggcgggt ggcagcggcg ccgtggcggc gccctccgcc
    11821 aaccgcttcg ggcgcatcag cccgaccatc gccgagcatg tgcaccagga actgggcgat
    11881 cgcgtggccc aggtcctgga cggcggcccc tgcgcggtgg ggctggagtc caccatcctc
    11941 gacctgtctc gcgatgtacc ggtgatcctg cggcccggcg ccattggcgc cgacgacatc
    12001 gcccgggtga tcggcctgcg accgtggcag cgtggcgaag cgcccgctgc gcccgacccg
    12061 gccgtgtcgc ccgcaccccg cgtttcgggt gctctggccg cccattacgc gccgcgcacg
    12121 ccgctggaac tggtgccggc agaccgcctc accgaacagg cagaggccgg tgacgcggtg
    12181 ctggcgcgtc gcccgtcgcc ggccggcctg cgccccgctg tcacctggat cgaggcgccg
    12241 gccgatgcca ccgcctacgg tcacgatctc tacgcccgcc tgcgcgccct ggatgcctcg
    12301 ggttgcgccc gcatcctcgt cgaggcgccc cccgacaccc ccgcctgggc ggccgtggcc
    12361 gaccgcctgg gccgcgcggc cgtgggttcc ggcgcgccgg aagcgaccta gacctgcccg
    12421 gccagattcc gggggcgccc ccacgcgctg cccgacgcgc gcgtggtcgc cgggatcccg
    12481 ttagggcccc ggccgatgga gcacggggcc tcggcaccgg gcccgccgga cggtctgggc
    12541 gatgcacttt ctgagtacgt tggttgcggg cggtgggttg atgcaaatgc ccggtctggg
    12601 acggccgggc gagggatgtc ggggacgccg tcagcgcgtc agcgctgcga ggaagtgttg
    12661 gcgcgccgcc tccagcgcgg ggcgggcgaa gtcatccacg tgacgcaggg cgaccgcgtg
    12721 atagacctcg aaggcggcgc agacggtatc caccgcgatc tgccgctccg ccgcaggcgg
    12781 tggcgcgggc gggagttcgc ccacggggcg caggaccgcg taacggggaa tcatgcgctg
    12841 tccgctcttt tcggaggcag cggtgatggg gccggtcttg gtgtagatct cgccttcgta
    12901 ttcgaagcgg gcgccaaggg ggatcaggtt gattttcatg ttcagggcct gcaagggggc
    12961 gggtggggcg cagcggggat tgtaatgaac ccggctgtga cttcccagcc ggcggtcagt
    13021 ggaggcgcgc tgtgcgcgat tggcgcggga cggtcgacgc ggaccggggc ggttgcggtc
    13081 cccatggcca tgaggagcgc gcgtccgtgt gccgacggtg ccggagctcg attgcgaatt
    13141 gctgcgcccg ggccgcgtgc cttgtcggtc ctggggcaca cgccgggcca tcgaagcggc
    13201 acgcaccgct tcagtgagga tccagctggc gccgtgccgc gggcacgaca accgctttgc
    13261 aagcgttgag cgctccgcta taatgcgcgg ctcaagtggg ccgatagctc agctgggaga
    13321 gcgccgcgtt cgcaatgcgg aggtcgtgag ttcgatcctc attcggtcca ccac
//
LOCUS       DAOQPA010000097        13355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2388_length_13355_cov_17.977293, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000097 DAOQPA010000000
VERSION     DAOQPA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_13355_cov_17.977293"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..692)
                     /locus_tag="PKX01_12840"
     CDS             complement(207..692)
                     /locus_tag="PKX01_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HMZ77061.1"
                     /translation="MPVVWRITTARFAASAFSGEGARRYGGRWNPRGTPLIYTAESRA
                     LALLEMLVQDDPLSARYVLIPAHIPDDLPLDRLDADALPADWRTLGARDALQTLGRRW
                     LEGPHRAALIVPSAVVPAEHNVLIDPTHPDFTRIAIGPVEALETDMRLLRNLAAQPRG
                     A"
     gene            complement(692..1144)
                     /locus_tag="PKX01_12845"
     CDS             complement(692..1144)
                     /locus_tag="PKX01_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HMZ77062.1"
                     /translation="MSATLLAFPGLSAAGLLGGEQLLHATPRLGIDWIPLVRRGLPAA
                     SLDALVRQTRIARTDLAEALAIPERTLARRKREGQLSPEESAKLVRLARTVERAEEVF
                     EDAAAALAWLRQDNAALGGVTPLSLLDTDLGADAVLDTLGRIEHGVFA"
     gene            complement(1338..4784)
                     /gene="mfd"
                     /locus_tag="PKX01_12850"
     CDS             complement(1338..4784)
                     /gene="mfd"
                     /locus_tag="PKX01_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMZ77063.1"
                     /translation="MTRPTHPFSALLPELDAVADARPGQRLDLPALAGSADALAIARL
                     AGGGRMLVVITAHPVDAQRLAEELAWLAPDLRTHLLPDWETLPYDSFSPHQDLISERL
                     STLYAITRGEADIALVPATTAVSRLAPPAFLAAYTFFLKQGEKLDVNGLREQMTVAGY
                     AHVTQVMSPGEYSVRGGLVDLYPMGAALPYRVDLFDEDIESIRTFDPDTQRTVFPVKE
                     VRLLPAREFPLDETGRTRFRGRFRETFEGDPSRCTIYKDISNGIAPAGIEYYLPLFFD
                     STATLFDYLPASAALFLHRDVAGAIDAFWKDVRSRHKLLDGDKTRPVMAPEQLFLGSE
                     SFFVALRDRPRLQLGTSAGDGPARPLPDVAVERKASDPLHRFKAFCAGFAGRVLVLAE
                     SPGRQQTIAEYFAEYGLSPSTTSDFDAFAGGDASLALATGPLAGGFVLPGAGLAVVTE
                     SELYAATARTRTRRENRKSASVEGWLRDLSELKAGDPVVHSSHGIGRYMGLVHMDLGE
                     GDTEFLHLEYANGDKLYVPVAQLHVITRYAGADPEAVHLHTLGSGQWEKAKKKAAQQV
                     RDTAAELLALYAQRAARKGHQFDFKQHDLEAFADGFGFEETPDQRAAIEAVVTDMKSG
                     RPMDRLVCGDVGFGKTEVALRAAFIAVADGKQVVVLCPTTLLAEQHYQTFSDRFADWP
                     IKVAELSRFKNAKEQADAIQQLGEGKVDILIGTHRLLQKDVVFKRLGLVIIDEEHRFG
                     VRQKEALKALRSEVDILTLTATPIPRTLGLALEGLREFSVIATAPQKRLAIKTFVQPY
                     NKGIVREAVLREFKRGGQVYFLHNEVETIVNVREALEELLPEARIVVGHGQLPERELE
                     RVMRDFTTQKANLLLCTTIIETGINIPTANTIIIHRADRFGLAQLHQLRGRVGRSHHQ
                     AYAYLLTDAHAKPTAQAQKRLEAISMMEELGSGFYLAMQDLEIRGAGEVLGENQSGEI
                     QQVGMSLYTEMLKRAVRDLQQGREPDLSQPLEVVSEINLHAPALLPSDYCSDVQERLT
                     LYKRLANCDSEDDLRALQEELIDRFGELPPQTRTLLETHRLRMLVRPYGVAKLDASDS
                     QITLHFGANAAIDPVKVILLIQKDKSVRMSGPDKLVRKVATPDLSQRVKAVRDLLVAV
                     KA"
     gene            complement(4861..5640)
                     /locus_tag="PKX01_12855"
     CDS             complement(4861..5640)
                     /locus_tag="PKX01_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HMZ77064.1"
                     /translation="MCLILVAWQARADYPLVVAANRDEFFARPAAAAAWWDDAPALFA
                     GRDLEAGGTWLGLSRQGRFAALTNFRDPARVRAGAPSRGALVADFLQGRESTDDALAR
                     LAAEGPRYNGFNLLLSDGVTLGVFESEADSGRGAGRRLAPGLYALSNHLLDTPWPKVS
                     AGKSRLARALDALPDDTPLRDLLRDDRPAPDAALPRTGVSLAWERMLSSAFIRAPGYG
                     TRCSTVIVRDRFGWTRVVESTWDAHGRESGRVAERFQIQPA"
     gene            complement(5631..5921)
                     /locus_tag="PKX01_12860"
     CDS             complement(5631..5921)
                     /locus_tag="PKX01_12860"
                     /inference="COORDINATES: protein motif:HMM:NF027614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HMZ77065.1"
                     /translation="MTPSHFYIWYPVRIGLEADLARTLYNLQSDVAERTGVAGRFLRK
                     ADDPWTWMEIYENVRDADAFARVLEEAVERHGLARFVDDGGHRHPERFVSCA"
     gene            complement(5929..6960)
                     /locus_tag="PKX01_12865"
     CDS             complement(5929..6960)
                     /locus_tag="PKX01_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HMZ77066.1"
                     /translation="MNAAWFTHLGAQGARFTDPYVRFHDDARQDVRAAEQGTVLVPLT
                     HLAAIRATGEDAGALLHNLFSNDVKKLGPNEAQLTSFNSAKGRMLASLLLWREGADYL
                     LALSADIHAAVLKKLGMYVLRSKAKLLDDSGDSVLIGLAGPQAGAALEAAGLGIPGTP
                     RATASGIATVVRLDNARFIVSAPLSEATQLWERLRASGAVPAGTAAWQWLDIREGLPT
                     VTLPVQEEFVPQMINYELIGGVSFNKGCYPGQEIVARTHYLGKLKKRMYRVHGDTADL
                     PAAGADLYAPEFGEQSAGKVVMAAPAPETGWDALVVLQTTTAEAGVVHLGAPDGPQLG
                     FLPLPYALT"
     gene            7146..8147
                     /gene="mltG"
                     /locus_tag="PKX01_12870"
     CDS             7146..8147
                     /gene="mltG"
                     /locus_tag="PKX01_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMZ77067.1"
                     /translation="MKRFIVRCLALGVALVALLAAALAWYAHTPVGLRTSPLDFTIEP
                     GAGMRQVARQVVDGGVEVQPALLTWLARITGQANSIKAGSYEVERGVTPLQLLAKLSR
                     GDVSQADLTLVEGWNIRQFRAALDRHPDLRHDSAGLSDAELLTRLGIDARHPEGQFFP
                     DTYLFARRSSDLEVLRRAHDNLQRRLAREWDKRADGLPYASPYEALIMASIVEKETGL
                     PADRPKIASVFVNRLRAGMLLQTDPTVIYGLGAAFDGNLRKRDLLADTPYNTYTRAGL
                     PPTPIAMPGLASLRAALNPPVTDYLYFVARGDGSSAFSRTLDEHNRAVAQYIRKRNS"
     gene            8144..8761
                     /gene="tmk"
                     /locus_tag="PKX01_12875"
     CDS             8144..8761
                     /gene="tmk"
                     /locus_tag="PKX01_12875"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMZ77068.1"
                     /translation="MKPRFITFEGIDGAGKSSQIAAAVALLRTRGLNVVQTREPGGTP
                     LGEKLRELLLHDAMHLETEALLMFAARREHLAALIEPALAAGSWVVSDRFTDATYAYQ
                     VGGRGLDEGRFAALERWVHPHLQPDLTFIFDLDPAIAAQRLAGTGVAPDRFEREQTDF
                     FRRVRAAYLRRAAADPGRVRVIDAGRTPDEIQKELEEIIVSHCLK"
     gene            8758..9822
                     /gene="holB"
                     /locus_tag="PKX01_12880"
     CDS             8758..9822
                     /gene="holB"
                     /locus_tag="PKX01_12880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HMZ77069.1"
                     /translation="MIFEWHKPLWQRVLDNSGRMHHALLLNGPAGGGKRAFAEALAAR
                     TLCVQPDADGQACGRCEDCQWRLAGSHPDFMRIIPEADQNDTESSSEKTGETKAASRQ
                     IVIDQIRDLQQSLTTGGHRGGQRVVIVDPAEAMNPYTANALLKLLEEPTTSTLFLLIT
                     STPKRLLPTIRSRCQSWAFPRPPADAAVRWLDAQQIAEAPALLAFAGGMPLAAAALAT
                     GGGAAALRRFTADIAGLPGKSALRLAGEWDAWLKSKEAQGCGLDMPTLTLWLQRWVGD
                     LASVCLGGPLRFFPDSRGVLQSLAERLAVDRVLGCYNDVTQSRRVAQHPLNARLFLED
                     MLLRYARAFSTGGARTGGGR"
     gene            9911..10279
                     /locus_tag="PKX01_12885"
     CDS             9911..10279
                     /locus_tag="PKX01_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ77070.1"
                     /translation="MNEPTKRATAARPSVLSLNINSKSALYAAYMPFVKNGAIFVPTV
                     RDYKLGDEIFMLLQIMDDSTKHPVAGTVVWVTPPNAQGNKTQGIGVSFAGDEPGQVLR
                     NRIEQILAGHLGSSRPTHTI"
     gene            10350..11123
                     /locus_tag="PKX01_12890"
     CDS             10350..11123
                     /locus_tag="PKX01_12890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ77071.1"
                     /translation="MFVDSHCHLDFPGLIEHEAEHLARMADNGVSHALCVSVKLETLP
                     AVIGLAERHAHLFASVGVHPDNADCDEPDEARLVALAAHPKVVAIGETGLDYYWHKDA
                     PEWQRQRFRTHIRAARACSKPLIVHTRSAAADTLRLMREEGADAVGGVMHCFTESLEV
                     AREALDLGFYISFSGIVSFKNAEDLRVVARMVPADRLLVETDAPYLAPVPFRGKTNQP
                     AYVRHVAEAVAAARGESLDAVADATTRNFFRLFKDAHAS"
     gene            11120..11791
                     /locus_tag="PKX01_12895"
     CDS             11120..11791
                     /locus_tag="PKX01_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ77072.1"
                     /translation="MTSHLLAVCRRAALAGAFATALCALPAGAATLDDVINAAKLGDA
                     AEIAQEVSRGLDPNVTDANGNTLLIIAAREDQPRVVEALLKYRVKINARNPVGDTALM
                     LAALRGNTRVVELLLQAGAAPDHSGWNPLLYASFEGHTAIVDMLLARGASPDATAPNG
                     ATALMFAAKGGHQDVVARLLKAGADTERANDRGDTAESWALQAHNTDIAEMIRAARSA
                     RATTR"
     gene            complement(11792..11944)
                     /locus_tag="PKX01_12900"
     CDS             complement(11792..11944)
                     /locus_tag="PKX01_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77073.1"
                     /translation="MICRVLMGGGLLAGVLLAGGQGEQALGPVVLGVLTAMVLRRMRP
                     LFPRRA"
     gene            12148..>13355
                     /locus_tag="PKX01_12905"
     CDS             12148..>13355
                     /locus_tag="PKX01_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMZ77074.1"
                     /translation="MKRAPPLPDSVPTDTRVPLRPLDELQVPPAFDGHDGINRADAAR
                     LQIAATTDPEAIACFLSEYDRSPGTYRIYQRECERLLLWAIVEVGKPLSSLNRQDFEG
                     YLNFLADPQPAVLWCGPKVARASEKWRPFVGPLSESAVLTAVAAINSLMSYLVDAGYL
                     AGNPLGLIRQRRRKMAAEAGGPLRAVASTDEVEKIERFLDAQMWDAVTAAIEALPRDS
                     ERERDEYERARFIAATLYLLAPRAGELESHRMNSFREERGLWWWHVVGKGAKKAKVPV
                     PEDMLQALIRYRKYLGLSAVPRRDDSTPLLISVKDRSPITARRLNQILKKIFHAAAEL
                     LPPEVEHKKEKLRAASAHWGRHTGITAKLDAGMDQRYVQKDARHTDARTTQRYIHEEE
                     ERWHDEAQKQ"
BASE COUNT         2064 a         4358 c         4862 g         2071 t
ORIGIN      
        1 caggggtgtt ctcggtgcga ggaagaggat caggccatgg acgagcaggg agatgcccag
       61 ggcgaagacc aggggcccgg tgcggcgcgg agggtcgggc gggaacatcg gcggcattct
      121 agcagcggtg ggttacggcg gcctgtcgtg gggcggatgc cgggaggccg gcggcacagg
      181 gtgcgggcgc gcccgtggcg gtgtgctcag gcgccgcgcg gctgcgcagc caggttgcgg
      241 agcaggcgca tgtccgtttc gagggcctcg acggggccga tggcgatgcg ggtgaaatcc
      301 gggtgtgtgg ggtcgatgag cacgttgtgt tccgcgggta ccacggcgct ggggacgatc
      361 agggcggccc ggtgggggcc ttcgagccag cgccggccga gggtctggag ggcgtcacgg
      421 gcgccgaggg tgcgccagtc ggcgggcagg gcgtcggcgt cgaggcggtc gaggggcaga
      481 tcgtcgggga tgtgcgccgg gatcagtacg tagcgggcac tcagggggtc gtcctggacg
      541 agcatttcga gcagggcgag ggcgcgggac tcggcggtgt agatgagggg tgtgccgcgg
      601 gggttccagc gtccgccgta gcgccgggcg ccttcgccgg agaaggccga tgcggcgaag
      661 cgggcggtgg tgatgcgcca gacgacgggc atcaggcgaa cacgccgtgc tcgatgcgtc
      721 cgagggtgtc gaggacggcg tctgcgccca ggtcggtgtc gagcagggac aggggcgtga
      781 cgccccccag ggccgcgttg tcctggcgca gccaggcgag ggcggcggcg gcgtcctcga
      841 agacttcctc ggcccgttcg acggtgcggg ccaggcgcac gagcttggcc gattcttcgg
      901 gcgacagctg gccttcgcgc ttgcgccggg ccagagtgcg ttccgggatg gcaagggctt
      961 cggccaggtc ggtgcgggcg atgcgggtct ggcgcacgag ggcgtccagg gacgcggcgg
     1021 gcaggccgcg ccggaccagg ggaatccagt cgatgcccag gcgcggcgtg gcgtggagga
     1081 gttgctcgcc gccgagcagg ccggcggcgg agaggccggg aaaggcgagg agggtggcgc
     1141 tcatggtcgg ggctccggtg agggcaggca ggccgcggca ggtgaggtgg tctgccattt
     1201 ggctttcaat ataggcaatt ttggcagaaa gcgctagaga gggcgatgcg cgatcgggcc
     1261 gcgcgctgcg ccagccgcga tgaactgcgg acgggcccgc cgccgccctg aacgcgggga
     1321 cccgaagcgg ggtgggctca ggccttgacc gcgacgagca ggtcccgcac cgccttgacc
     1381 cgttggctca gatctggcgt ggcgaccttg cgcacgagct tgtccgggcc ggacatgcgc
     1441 acgctcttgt ccttctggat cagcaggatg accttgaccg ggtcgatggc ggcgttggcg
     1501 ccgaagtgga gggtgatctg gctgtcggag gcgtccagct tggccacgcc gtaggggcgc
     1561 accagcatgc gcaggcggtg ggtctcgagc agggtgcggg tctggggcgg cagttcgccg
     1621 aagcggtcga tgagctcttc ctgcagggcg cgcaggtcgt cctcgctgtc gcagttggcg
     1681 aggcgcttgt agagggtgag gcgttcctgc acgtcgctgc agtagtcgct gggcagcagg
     1741 gcgggggcgt ggaggttgat ctcggagacc acctccaggg gctgggagag gtccggttcg
     1801 cggccctgtt gcaggtcgcg cacggcgcgc ttgagcatct cggtgtagag gctcatgccc
     1861 acctgctgga tctcgccgga ctggttttcg ccgagcacct cgccggcgcc acggatctcc
     1921 agatcctgca tggccaggta gaagccggag cccagctctt ccatcatgga gatggcctcc
     1981 aggcgcttct gggcctgggc ggtgggcttg gcatgggcgt cggtgaggag gtacgcgtag
     2041 gcctggtggt gggagcgccc gacgcggccg cgcagctggt ggagctgggc cagtccgaag
     2101 cggtcggccc ggtggatgat gatggtgttg gcggtgggga tgttgatgcc ggtctcgatg
     2161 atggtggtgc acagcagcag gttggccttc tgggtggtga agtcgcgcat gacgcgctcc
     2221 agctcccgct ccggcagctg gccgtgaccg accacgatgc gcgcctcggg gagcagttcc
     2281 tccagggctt cgcggacgtt gacgatggtc tccacctcgt tgtggaggaa atacacctgg
     2341 ccgccgcgct tgaattcccg cagcacggct tcgcgcacga tgcccttgtt gtagggctgg
     2401 acgaaggtct tgatggccag gcgtttctgg ggcgcagtgg cgatcaccga gaattcccgc
     2461 agaccttcca gggccaggcc cagggtgcgc gggatggggg tggcggtgag ggtgaggatg
     2521 tccacctcgc tgcgcagggc cttgagggct tccttctgac gcacgccgaa gcggtgctcc
     2581 tcgtcgatga tgaccaggcc caggcgcttg aagaccacgt ccttctggag caggcggtgg
     2641 gtgccgatga ggatgtccac cttgccttcg cccagctgct ggatggcgtc ggcctgttcc
     2701 ttggcgttct tgaagcggga cagctcggcc accttgatgg gccagtcggc gaagcggtcg
     2761 gagaaggtct ggtagtgctg ctcggcgagc agggtggtgg ggcacaggac gaccacctgc
     2821 ttgccgtcgg ccaccgcgat gaaggcggcg cgcagggcga cctcggtctt gccgaagccc
     2881 acgtcgccgc acaccaggcg gtccatgggg cggccggact tcatgtcggt caccacggcc
     2941 tcgatggccg cgcgctggtc gggggtttcc tcgaagccga agccgtcggc gaaggcttcc
     3001 aggtcgtgtt gcttgaagtc gaactgatgg cccttgcggg cggcgcgctg ggcgtagagg
     3061 gcgaggagct cggcggccgt gtcgcgtacc tgctgggcgg ccttcttctt ggccttctcc
     3121 cactgaccgg agccgagggt gtgcaggtgc accgcctccg ggtcggcgcc ggcgtagcgg
     3181 gtgatgacgt ggagctgggc gacggggacg taaagcttgt cgccgttggc gtattccagg
     3241 tgcaggaatt ccgtgtcgcc ttcgcccagg tccatgtgca ccagacccat gtagcggccg
     3301 atgccgtggc tggaatggac caccggatcg ccggccttga gctccgacag gtcgcgcagc
     3361 cagccttcca cgctggcgga cttgcggttc tcgcggcgcg tgcgggtgcg cgcggtggcg
     3421 gcgtagagct cggattcggt gaccaccgcg aggccggcgc cgggcagcac gaatccgccg
     3481 gccaggggcc cggtggcgag ggccagggat gcatcgccgc cggcaaaggc gtcgaagtcg
     3541 cttgtggtgc tggggctcag cccgtattcg gcgaagtatt cggcgatggt ctgctgacgc
     3601 cccggcgatt cggccagcac cagcacccgg cctgcaaagc cggcgcagaa ggctttgaag
     3661 cggtgcagcg ggtcgctggc cttgcgctcc accgccacgt cgggcagggg ccgtgccgga
     3721 ccgtcgccgg cggacgtgcc cagttgcagg cgggggcggt cgcgcagggc gacgaagaag
     3781 ctctcgctgc ccaggaagag ctgctccggc gccattaccg gacgggtctt gtccccgtcc
     3841 aggagcttgt ggcggctgcg tacgtccttc cagaaggcgt cgatggcgcc cgccacgtcc
     3901 cggtgcagga acagggcagc gctggcgggc aggtagtcga agagggtcgc cgtggagtcg
     3961 aagaagaggg gcaggtagta ctcgatgccg gctggcgcga tgccgttgga gatgtccttg
     4021 taaatggtgc agcgggacgg atcgccttcg aaggtttcgc ggaaacggcc gcggaaccgg
     4081 gtgcggccgg tttcgtcgag gggaaactcc cgcgccggca ggaggcgcac ctccttgacc
     4141 gggaagacgg tgcgctgggt gtccgggtcg aaggtgcgga tgctctcgat gtcttcgtcg
     4201 aagaggtcga cccggtaggg cagcgcggcg cccatgggat agagatccac cagcccgccg
     4261 cgcacgctgt attcgccggg cgacatgacc tgggtgacgt gggcgtagcc ggcgacggtc
     4321 atctgctcgc gcaggccgtt cacgtcgagc ttttcgccct gtttcaggaa gaaggtgtag
     4381 gccgccagga aggccggcgg ggccaggcgc gagacggcgg tggtggccgg cacgagggcg
     4441 atgtcggctt cgccgcgggt gatggcgtag agggtggaca ggcgctcgga gatcaggtcc
     4501 tggtgaggcg agaagctgtc gtagggcagg gtctcccagt ccggcagcag gtgggtgcgc
     4561 agatcaggcg cgagccaggc gagctcttcc gccagacgtt gggcgtccac ggggtgggca
     4621 gtgatcacca ccagcatgcg cccgccgccc gccagacggg cgatggccag ggcgtcggcg
     4681 gatccggcca gggcgggcag atccaggcgc tggccgggac gggcgtcggc cacggcgtcg
     4741 agttcgggca ggagggcgct gaaaggatgc gtggggcggg tcatgcgggg ttcgcggtgc
     4801 ggcgcggagg gggttccggg gaagggcgga ttataggcgt ccgcgccggg cgtgcgggat
     4861 tcaggcgggc tggatctgga agcgctcggc cacccggccg ctttcccggc cgtgggcgtc
     4921 ccaggtggac tcgacgaccc gggtccagcc gaaacggtcg cggacgatca ccgtggagca
     4981 acgcgtgccg tagcccggag cgcggatgaa ggcgctggac agcatgcgct cccaggcgag
     5041 gctgacgccg gtgcgcggca gggcggcgtc cggtgcgggg cggtcgtccc gcagcaggtc
     5101 tcgcaggggc gtgtcgtcgg gtagggcgtc gagggcgcgg gcgaggcgcg acttgccggc
     5161 gctgaccttg ggccagggcg tgtccagcag gtggttggag agcgcgtaga ggcccggtgc
     5221 gaggcgccgc ccggcgccgc ggccgctgtc ggcctcgctc tcgaagacgc ccagcgtgac
     5281 accgtccgac agcagcaggt tgaagccgtt gtagcgcggg ccttccgccg cgaggcgggc
     5341 gagggcatcg tcggtggatt cgcgaccctg caggaaatcc gccaccagcg cgccgcggga
     5401 gggggcgccg gcgcgcacgc gggcgggatc gcggaaattg gtgagggcgg cgaagcggcc
     5461 ctggcggctc aggccgagcc aggtgccgcc cgcctccagg tcgcgcccgg caaacagggc
     5521 cggcgcatcg tcccaccagg cggctgcggc ggccgggcgc gcgaagaact catcccggtt
     5581 ggccgccacc accagcgggt agtcggcgcg ggcctgccag gcgacgagga tcaggcacac
     5641 gacacgaaac gttccggatg gcggtgcccg ccgtcgtcca cgaagcgggc caggccatgg
     5701 cgctcgacgg cttcttcgag cactcgggcg aaggcgtcgg cgtcgcgcac gttctcgtag
     5761 atctccatcc aggtccacgg gtcgtcggcc ttgcgcagga agcgcccggc gacgccggtg
     5821 cgctcggcca cgtcgctctg caggttgtag agggtccggg ccagatcggc ctcgaggccg
     5881 atccgcaccg gataccagat gtagaaatgg gacggggtca cgggaccgtc aggtgagagc
     5941 gtagggcagg ggcaggaaac cgagctgcgg accgtcgggc gcgcccagat ggacgacgcc
     6001 ggcctcggcg gtggtggtct gcagcaccac cagggcgtcc cagccggtct ccggtgccgg
     6061 ggcggccatg acgaccttgc ccgcggattg ctcgccgaat tccggtgcgt agaggtcggc
     6121 cccggcggcg ggcagatcgg ccgtgtcgcc atgcacgcgg tacatgcgct tcttgagctt
     6181 gcccaggtag tgggtgcggg cgacgatttc ctgaccggga tagcagccct tgttgaagct
     6241 cacgccgccg atcagctcgt agttgatcat ctggggcacg aattcctcct gcacgggcag
     6301 cgtcacggtg ggcaggccct cgcggatgtc cagccactgc caggcggcgg tgccggcggg
     6361 cacggcaccg ctggcgcgca ggcgttccca cagctgggtg gcttcggaga ggggcgcgga
     6421 gacgatgaag cgggcgttgt ccagacgcac cacggtggcg atgcccgacg cggtggcccg
     6481 cggcgtgccg gggatgccca gaccggcggc ttccagcgcg gcgcccgcct gcggaccggc
     6541 gaggccgatc agcacgctgt cgccgctgtc gtccagcagc ttggccttgg agcgcagcac
     6601 atacatgccg agcttcttga gcacggcggc gtggatgtcg gcggaaaggg ccagcaggta
     6661 gtcggcaccc tcgcgccaca gcagcaggct ggcgagcatg cggcccttgg cgctgttgaa
     6721 gctggtgagc tgggcctcgt tggggccgag cttcttcaca tcattggaga acaggttgtg
     6781 gagcagggcg ccggcatcct cgccggtggc gcggatggcg gcgagatggg tcaggggcac
     6841 cagtacggtg ccctgttcgg cggcgcggac gtcctgccgc gcgtcgtcgt ggaagcggac
     6901 gtacgggtcg gtgaagcggg cgccctgggc gccgagatgg gtaaaccagg ctgcgttcat
     6961 ggtggtcctg tgggcgagcg aactcgaagg gtcgggagcg aggatgtgtg agctttgggc
     7021 tattatacgg gcgccttccg gccggtatgc acgatcggtg ccaccttcgt cacaccggcc
     7081 gtgcagtccg cagtcattgt ctcgtccctc ctctttcgtt ccttccggac gcgccaagtt
     7141 cccgtatgaa acgtttcatc gtccgctgtc ttgccttggg cgtcgcgctc gtcgcgctgc
     7201 tcgccgctgc cctcgcgtgg tatgcccaca cgccggtggg gctgaggacc agcccgctgg
     7261 atttcaccat cgagcctggc gccggcatgc gtcaggtggc ccgccaggtg gtggacggcg
     7321 gcgtcgaggt gcaaccggcg ctgctcacct ggttggcgcg gatcaccggt caggccaact
     7381 ccatcaaggc cggcagctac gaagtggaac gcggcgtgac gcccctgcaa ctgctcgcca
     7441 agctcagccg cggcgacgtg agccaggcgg atctgaccct cgtggaaggc tggaacatcc
     7501 gccagttccg cgccgcgctg gaccgtcacc cggatctgcg ccacgacagc gccgggctca
     7561 gcgacgcgga actgctgacc cgcctgggca tcgacgcgcg tcacccggaa gggcagttct
     7621 tcccggatac ctatctcttc gcgcgccgct ccagcgatct ggaggtgctg cgccgggccc
     7681 acgacaacct gcagcggcgc ctcgcccggg aatgggacaa gcgtgccgat ggcctgccct
     7741 acgcgtcgcc ctacgaagcc ctgatcatgg cctccatcgt agaaaaggag accggcctgc
     7801 ccgccgaccg gcccaagatc gcgtcggtct tcgtcaaccg cctgcgcgcc ggcatgctgc
     7861 tgcagaccga tccgacggtc atctacggcc tgggcgcggc cttcgacggc aacctgcgca
     7921 agcgcgacct gctcgccgac accccttaca acacctacac ccgcgccggc ctgccgccca
     7981 cgcccatcgc catgcccggg ctggcgtccc tgcgggcggc cctcaatccg ccggtgaccg
     8041 actatcttta cttcgtggcc cgcggtgacg gttccagcgc gttctcgcgc acgctggacg
     8101 aacacaatcg ggccgtcgcc cagtacatcc ggaaacgtaa ctcgtgaaac cccgcttcat
     8161 caccttcgaa ggcatcgacg gcgccggcaa gagcagccag atcgcggcgg cggtcgcctt
     8221 gctccgcacc cgcggcctga acgtcgtgca gactcgcgag ccgggcggca cgccccttgg
     8281 cgaaaaactg cgggagctcc tgctgcatga cgccatgcac ctggaaaccg aggccctgct
     8341 gatgttcgcg gcgcgccggg agcacctcgc ggccctcatc gagccggccc tcgcggcggg
     8401 cagctgggtg gtctccgacc gcttcacgga cgccacctac gcctaccagg tgggggggcg
     8461 cggcctggac gaagggcggt tcgctgcgct ggagcgctgg gtgcatcccc acctgcagcc
     8521 ggacctgacc ttcatcttcg atctggaccc cgccattgcc gcgcaacggc tcgcgggtac
     8581 cggcgtggcg ccggatcgtt tcgagcggga acagacggat ttcttccggc gggtacgcgc
     8641 tgcctacctg cgccgggccg cggccgatcc agggcgggtc cgggtgatcg acgccggacg
     8701 tacgccggac gaaatccaga aggaactcga agaaatcatc gtaagtcatt gcctgaaatg
     8761 atttttgagt ggcataaacc tctctggcag cgcgttttgg acaactccgg acggatgcac
     8821 catgccttgc tgctgaatgg tccggcgggc ggcggcaaac gtgccttcgc cgaagcgctg
     8881 gcggcgcgga cgctgtgcgt gcagccggat gcagacggtc aggcctgcgg gcggtgcgaa
     8941 gactgtcagt ggcgtctggc agggagtcat cctgatttta tgcgcatcat tcctgaagca
     9001 gatcaaaatg atacggaatc ttcttcagaa aaaactggag aaacaaaggc ggccagtcgg
     9061 cagatcgtca tcgaccagat ccgcgacctc cagcaaagtc tcaccaccgg cggccatcga
     9121 ggcggacagc gggtcgtcat cgtcgatccg gccgaggcga tgaatcccta caccgccaat
     9181 gcactgctca agttacttga agaacccact acaagtactc tgtttttatt gattacatcg
     9241 acgccgaaac gactcctgcc gacgatccgc agccgctgtc agagctgggc cttcccgcgt
     9301 ccgccggccg acgctgccgt gcgctggctg gacgcgcagc agatcgcgga ggcgcccgcg
     9361 ctgctggcct ttgcaggggg catgccgctg gctgcggcag ccctcgcgac aggcggcgga
     9421 gcggcggcat tgcggcgttt cacagcagat atcgccggcc tgccgggcaa gtcggccctg
     9481 cgtctggcgg gggagtggga tgcctggctc aaaagcaagg aggctcaggg ctgcgggctg
     9541 gacatgccga ccctgaccct gtggctgcag cgctgggtgg gggacctggc cagcgtgtgc
     9601 ctgggcgggc cactgcgttt ctttccggac agccggggtg tgctgcagag tctggcggaa
     9661 cgcctcgccg tggatcgggt gctgggctgc tacaatgatg tgacccagtc gcgccgggtg
     9721 gcccagcatc ccctgaacgc gcggcttttc ctcgaagaca tgctgttgcg gtacgcccgg
     9781 gccttcagca cgggcggtgc gcggaccggt ggcgggcggt gagcgcccgg gcctccggag
     9841 cggcagccag gcggctgcgc gccgtgcatc gtttggcccc ttgcaatgac cgccggccgg
     9901 tggagtacgc atgaacgaac ccacgaagcg cgcgaccgca gcccgtccga gcgtgctgtc
     9961 gctcaacatc aattccaagt ccgcgctcta cgcggcttac atgcccttcg tcaagaacgg
    10021 cgcgatcttc gtgcccacgg tgcgggacta caagctcggc gacgagatct tcatgctcct
    10081 gcagatcatg gacgattcca ccaagcaccc ggtggccggc acggtcgtgt gggtcacgcc
    10141 gcccaacgcc caggggaaca agacccaggg catcggggtc agtttcgctg gtgatgaacc
    10201 cgggcaggta ttgcgtaacc gcatcgagca gatcctcgcc ggccatctgg gctcctcccg
    10261 tcccacgcat accatctgag cggcgcgcct gaacacgatc cactggtgcc cgtgccgcac
    10321 gcccccttcg ttgtgagcgc acactcttca tgtttgtcga ttcccactgt cacctggatt
    10381 tccccggact catcgaacac gaggccgagc acctcgcgcg catggccgac aacggtgtga
    10441 gccacgcctt gtgtgtcagc gtcaagctcg aaaccctgcc ggcggtgatc gggctggcgg
    10501 agcgtcacgc ccacctgttt gcgtccgtgg gggtgcatcc ggacaacgca gactgcgacg
    10561 aaccggatga agcccgcctc gtggccctgg cggcccaccc caaggtggtg gccatcggcg
    10621 agaccgggct ggactactac tggcacaagg acgcgccgga atggcaacgg cagcgctttc
    10681 gcacccacat tcgtgccgcc cgcgcgtgct ccaagccgct catcgtgcat acccgcagtg
    10741 ccgcggccga caccctgcgc ctgatgcgcg aggagggcgc agatgccgtt ggtggtgtga
    10801 tgcactgctt caccgaaagc ctggaggtgg cccgggaggc cctggatctg ggcttctaca
    10861 tctccttttc cggcatcgtc agcttcaaga acgcagaaga tctgcgggtc gtcgcccgca
    10921 tggtaccggc cgaccgtctg ctggtggaaa ccgacgcgcc ctacctggcg ccggtgccct
    10981 tccgtggcaa gaccaaccag cccgcttacg tccgccatgt ggcggaagcc gtcgcggcgg
    11041 cccggggcga aagccttgac gccgtggccg acgcaacgac ccggaacttc ttccggctct
    11101 tcaaggacgc ccacgcctca tgacatcgca tctcctcgcc gtctgccgcc gtgccgcact
    11161 ggccggcgcc tttgccaccg ccctgtgcgc gttacctgcg ggcgcggcca ccctcgacga
    11221 cgtgatcaat gccgccaagc tgggcgacgc cgccgagatc gcccaggagg tgagccgtgg
    11281 cctggacccc aacgtcaccg acgcgaacgg caacaccctg ctcatcatcg cggcccggga
    11341 agatcagccc agggtggtgg aggcgctgct caagtaccgg gtgaagatca acgcccgcaa
    11401 cccggtcggt gacacggccc tgatgctggc cgcgctgcgc ggaaatacgc gcgtcgtgga
    11461 actcctcctc caggccggtg cggcaccgga tcattccggc tggaatccac ttctgtacgc
    11521 cagttttgaa gggcataccg ctattgtcga catgctcctc gcccgcggcg caagcccgga
    11581 tgccacggcg cccaacggcg ccacggcgct gatgtttgcc gccaagggcg gtcaccagga
    11641 cgtggtggcg cgcctgctca aggccggagc cgacaccgag cgcgccaacg accggggcga
    11701 caccgccgaa agctgggccc tgcaggccca caacaccgac attgccgaga tgatccgcgc
    11761 tgcccgcagc gcccgcgcca ccacccgttg atcaggcccg acgcgggaac aggggccgca
    11821 tgcggcgcag caccatggcg gtcagcacgc ccagcaccac cggccccagg gcctgttcgc
    11881 cctggccacc ggccagcaac acaccggcca gcagcccgcc gcccatcagc acacgacaga
    11941 tcatgttttc catcatcttc tcctgagtag gggaacggac agcggccctg cgcatccggg
    12001 gcgccttcgg cgatccagag ggaaggccgg attcatccgt tcgataagtg taattatata
    12061 cagctacgtg tgggatgcaa gggtgcgtgc gccgatcggt acatcggcgt atcctgcgac
    12121 gcgcccccgc ccgatcgccc tacgcccatg aagcgcgcgc ccccactgcc cgactccgtt
    12181 ccgaccgaca cccgcgtgcc cctgcgtccg ctggacgaac tgcaggtgcc gccggccttc
    12241 gacggccacg acgggatcaa ccgcgccgac gccgcgcgcc tgcagatcgc cgcgaccacg
    12301 gaccccgaag ccatcgcctg cttcctcagt gagtacgacc gctcgcccgg cacctaccgc
    12361 atctaccagc gggaatgcga gcgcctgctc ctgtgggcca tcgtcgaggt gggcaagccc
    12421 ctgtccagtc tcaaccggca ggatttcgag ggctacctga acttcctcgc cgatccccag
    12481 ccggcggtcc tgtggtgcgg gcccaaggtg gcgcgggcgt cggaaaagtg gcggcccttc
    12541 gtcggccccc tcagcgagag cgccgtgctc accgccgtgg cggcgatcaa ttccctgatg
    12601 agctacctgg tggatgcggg ctatctggcc ggcaatcccc tcgggctcat ccgccagcgc
    12661 cgtcgcaaga tggccgccga ggcgggcgga ccgctgcggg ccgtggccag caccgacgag
    12721 gtggagaaga tcgagcgctt cctcgacgcc cagatgtggg acgccgtcac cgccgccatc
    12781 gaggccctgc cgcgggattc cgagcgggag cgggacgaat acgagcgcgc ccgcttcatt
    12841 gccgccaccc tctacctcct cgcgccccgc gcaggcgagc tggaaagcca ccgcatgaac
    12901 agcttccgcg aggagcgcgg cctatggtgg tggcacgtgg tgggcaaggg cgccaagaag
    12961 gccaaggtgc cggtgcccga ggacatgctc caggccctca tccgctaccg caaatacctg
    13021 ggcctcagcg ccgtgccccg ccgcgacgac agcacgccgc tgctcatctc ggtgaaggac
    13081 cgcagcccca tcaccgcccg ccgcctcaac cagatcctca agaagatctt ccacgccgcc
    13141 gccgagctgc tgccgcccga ggtggagcac aagaaggaaa agctccgcgc cgcctccgcc
    13201 cactggggcc gccacacggg catcaccgcc aagctggacg ccggcatgga ccagcgctac
    13261 gtgcagaagg acgcccgcca cacggacgcc cgcaccacgc agcgctacat ccacgaagag
    13321 gaagagcgct ggcacgacga ggcacagaag cagaa
//
LOCUS       DAOQPA010000098        13282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2402_length_13282_cov_18.847433, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000098 DAOQPA010000000
VERSION     DAOQPA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_13282_cov_18.847433"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKX01_12910"
     CDS             <1..201
                     /locus_tag="PKX01_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77075.1"
                     /translation="SGRVQTYRDDQAPTLTGTVTPGPRNRDAAGSLCVTNTDYLADRG
                     KGENVYVKMCQQADGGWAPAVS"
     gene            206..1102
                     /locus_tag="PKX01_12915"
     CDS             206..1102
                     /locus_tag="PKX01_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77076.1"
                     /translation="MNARFLAGARRLCGLAVLLAVAPLPAVALDFTPVTLDNGLKLLI
                     VHDKRPCARNESCGFTAGDANRLKDFLERDRYVQVLLNSNGGSSTEGQRVGAVLRARG
                     AWVRVAKGHHCISACTAAFLGGVIREVDEGGEFHVHSSSSYLNGVSEEEWQRVSRDPD
                     LELGNTLRATVADRVEWTLSRFAYVQRMIAGESPKGLLEGSNSECLSRAQDQLLQPGR
                     LRQLAQRVRVEGRPALNEIEEATERRTTELCVGILTAQADRLGPRAPQVLRIMDIMTA
                     SRIGATINLSPQALREAGFVTQ"
     gene            1099..2313
                     /locus_tag="PKX01_12920"
     CDS             1099..2313
                     /locus_tag="PKX01_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77077.1"
                     /translation="MMRLNSTTLNVLIASLLCGGCASTSVSDSLWTQVSGDGNRIVLR
                     LDPEHPRLRQFASAQINLAADFVDDSGTPRTASLGGGSRVDAQTRVYTLPETLNNPPS
                     GEICLYFSPADTRLGAFPIRARTAGGTDNSGFRYPDWEARVGAETRRRLNDRQKSGLD
                     AQRNAAQRSVQQLQAELDKDQLASVEDCARRTFTAKADARGRDVIEPAAQAATARRIC
                     VRRTRELNLTSKFSVVDALGQFRNQLPSERSRQAGQFLTDWRNHFERTGADYVPELGS
                     PREPLAIATDAETLAARPSLQPKDSIALIGAWLDAYDVCVQDTTHQLGHRYQLWQVSQ
                     GRSSDRAAAYSDYMAQECRGKFRRLNEQRALLADVDRQLGALPSAAPTSSPQAQNRNV
                     SLNALACTLPPR"
     gene            complement(2374..3801)
                     /locus_tag="PKX01_12925"
     CDS             complement(2374..3801)
                     /locus_tag="PKX01_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ77078.1"
                     /translation="MKTCLRPLPVILVVLLSACRIGPDYVRPEMAVPTVYKEAGDWKP
                     AAPADPAAGDAWWTAFGDPVLDQLQARLLVSNQNLRAVEAQYRQARALADSARASGFP
                     TVTGSVGMTRGTSGSSVSSTASQGPAAVTNSYSLGASSSWEADVWGRIARNVEAADAR
                     VEASDADLAAARLSARATLAQTYFQLRSAERQKRLLEATVAAYERALELTRNRYAAGV
                     AGRADVVQAESLLRSTRAQTIDVRLSRAQYEHAIAVLIGEAPAAFTLAESDAPLPVAV
                     TPVSLPSTLLERRPDIAAAERRVAAANAAVGAAQAARFPVLGLTAGAGYRSSDLSELL
                     SLSSRYWSLGPTVAASLFDGGAKRAATGQAVAVWDQAVATYRQTVLTAFQEVEDNLVA
                     ARLLEHELTEQNAAVTAAAEAEAIALNQYRAGTVSYLNVVTAQTTLLTARRSANDLAA
                     RRLLAAVLLAKNAGGDVARSAKPAP"
     gene            complement(3798..6908)
                     /locus_tag="PKX01_12930"
     CDS             complement(3798..6908)
                     /locus_tag="PKX01_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HMZ77079.1"
                     /translation="MNLSRLFIFRPVATSLLTLAIALAGAIAFRLLPVSPLPQVEYPT
                     ISVSASLPGASPETMAATVAGPLERILGQIAGVNEMTSTSTLGSSRVTLQFDLSRDID
                     GAARDVQAAINAARGQLPTSLPNNPTYRKVNPSETPILILALTSTTLTQGQLYDVAST
                     VLAQRLAQVDGIGQVSIGGSSLPAVRVEANPDRLAAAGIALDDVRTAIVGAVANRPKG
                     VLDDGQRAWQIGANDQAYTAREYRPLIVTWKNGAAVRLGDVARVRDSVQDERNFGSAN
                     GRPAVLLILNRQPGANIIATVDRVTALLPHLRALVPAAVDLDVVMDRTPTIRGSLREV
                     ERTLVISVGLVVMVVFLFLRNVRATVIPSIVVPVSLIGTFAIMYLAGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENINRHVEDGHTPLRAALRGGREIGFTVISMSLSLIAVFIPIL
                     AMGGIVGRLFREFAVTLSAAILVSMVVSLTATPMMCARLLRPTPPAAGRGRFDRWIGG
                     AFERLLAGYRHSLGWALDHPRLILAVLLLTVGCNVYLYNAIPKGFFPQQDTGRIRGFV
                     EADQAISFQAMRGKMERLLALVGADPAVDNVVGFTGGFQLNYGSMFVTLKPLGVRAES
                     ADAVVARLRRQLAHFPGANLYLNPVQDIRIGGRSSNSTYQFTLQADRLDDLRLWEPRI
                     RNAMSRLDELTDVNTDQQDRGPQTSLVVDRDAMTRYGLTMRSLTTALNNAFGQRQVST
                     IYNPLNQYRVVLEAAPAYLQDPDVLSRLTLVASGGQPVPLSAVARFESTLAPLSVNHQ
                     DGAVASTISFNLPEGKTLGEATRAIDAALQQIGVPVSIRTSFQGSAGAFQQSMSTQPL
                     LILAALISIYIVLGMLYESLVHPLTILSTLPSAGVGALLALLAVNMEFSLIAMIGVIL
                     LIGIVKKNAIMMIDVALALERSEGVGARAAIHHACLLRFRPILMTTLAAICGAVPLAI
                     GTGDGAELRQPLGIAIVGGLVLSQILTLYTTPVVFVLLDRLRSRAGAARAPVPDLARS
                     PAGNLS"
     gene            complement(6905..9991)
                     /locus_tag="PKX01_12935"
     CDS             complement(6905..9991)
                     /locus_tag="PKX01_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HMZ77080.1"
                     /translation="MNPSRLFIVRPVATALLMVAILLTGLVAYRLLPIAALPQVDYPT
                     IQVKTFYPGASPDVMTSAITAPLERQFGQMPGLDEMSSTSSGGASVITLRFSLDLTLD
                     VAEQQVQAAINAASNLLPTDLPAPPTYSKVNPADTPILSLAISSPSLPLTRVHDLVES
                     RLAQKIAQVPGVGLVSIAGGRRPAVRVQVNPTQLAAYGLALEDVRTAIANANITQAKG
                     SFDGPQRASTIDANDQLRSADDYRKLIVTYRNGSPVRLDDVANLKDDAENLRLAAWAD
                     TTGAVIVNVQRQPGANVIEVADRVRALIPQMTASLPASLDVRVLSDRTTTIRASVHDV
                     QVELALAVALVVMVIFLFLRSVPATLIPSVAVPLSLVGTFGVMYLAGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYVEEGDSPMEAALKGSRQIGFTIISLTLSLIAVLIPLL
                     FMADVVGRLFREFAVTLAIAILISAFVSLTLTPMMCARLLRHVPEAQQGRFARAAGDF
                     IDRVIARYGRMLAWVLDRQGATLLVALATLGLTAALYILVPKGFFPVQDTGAIQGITE
                     APQTISFAAMAERQQAVAAVILRDPAVASLSSFIGVDGTNATLNSGRLLISLKPLAER
                     GVTASEVIRRLAPELARVAGITLYMQPVQDLSIEDRVSRTQYQFSLQSPDAAALAEWS
                     PRLVERLRALPQLADVASDLQDKGLQAYVDIDRDAAGRLGVTTAAIDNVLYNAFGQRL
                     VSTIFTQASQYRVVLEVLPEFRTRPEALAKLYVPTLAGVQVPLSAVARVSERPTTLAV
                     NHIGQFPAVTLSFNLAPGVALGDAVEAIRRAEAELGLPASIETGFEGAALAFQSSLSS
                     TLLLILAAVVTMYIVLGVLYESTIHPVTILSTLPSAAVGALLALLLARAELDITAVIG
                     IVLLIGIVKKNAIMMIDFALEAERHQGMRPREAIYQACLLRFRPILMTTLAALLGALP
                     LMLGTGVGSELRHPLGITLVGGLMVSQVLTLFTTPVIYLAFDRLSLRLQRRTATAP"
     gene            complement(10004..11284)
                     /locus_tag="PKX01_12940"
     CDS             complement(10004..11284)
                     /locus_tag="PKX01_12940"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HMZ77081.1"
                     /translation="MSPTPSDVSPAASANQPVSPPRRRLWPWFLVLALALGGVYAWRT
                     SMSESKVHAEDRGRNRGDRPQPVLLGEVQAGDIRVYVSALGSLVPRNQVTVRARVDGQ
                     LMKVVFREGQAVKAGDLLAEVDARPFEVAVAQAEGQLLRDKALLQNARIDLDRYKGLL
                     AQDSIARQQVDTQEALVRQYEGTVKADQGAVDSARLQLSYTRITAPIAGRAGLRQVDP
                     GNIVHASDTTGVVTLAQVSPITAVFAVPEERVAEVMARQKAGKPLAVEAWDREQRTLL
                     ATGRLIAADSQIDTTTGTLKLKAEFANEDGSLFPNQFVNVRLILDRLKAVPVLPTAAL
                     MQGDKGRYVYRVGADNTVAAVTVRLGAVDGERIAVEGGLDLGDKVVVDGLDKLRDGAA
                     VEAIAPDAANAPGRKRPPKGGPGGGGPGGGRPAH"
     gene            11580..>13282
                     /locus_tag="PKX01_12945"
     CDS             11580..>13282
                     /locus_tag="PKX01_12945"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ77082.1"
                     /translation="MKISFRRSACTIAAAILLPVTHAQGSPDLSGLLSILNSTPEGTW
                     VQVNLNSYSEAWTPAELRPLYLTSNPDPSKITYAWPSFAWDSNRGDLILYGGGHANYS
                     GNDVYRWHGTTRKWERAALPSELLLNPPGFYTPIDGPDAGPPSSHTYDNSLFLPIIDR
                     FIVFGGAAFNTGGPYNRMNPDGVTYRNTGPYLFDPSKADPNKVGGTTGSHVKRVAPHP
                     EIVGGNMWTNRDYRAYTFTGSTLIPNSHINGVTAYAQENGKDVVYFGARPGQGTDQHL
                     FKYTISSLTDVTQDKLEKVGRFWNGPSDQGAGTYDPTRKLFVRTGPATQPITYWDLNN
                     ASPSNNDVVTVPVDASGTFDFNQMRMTGIDYDPTTDQYLIWYGGPDVWRLTPPTPVTS
                     TGWKLDKVSKTSSAAPTIDADSRGILGKWKFIPNLNAFLGLQDFVNGNIWLFKPVGWK
                     LPTGTGNLPPTVSLTSPSAGASFAVGSIVSVTATASDSDGSVTQVAFYVDGGIATTSS
                     GPTYTANLSGLTAGTHSLTAVATDNAGATTTSAAVSITVTSATNTPPTVSLTSPAAGA
                     S"
BASE COUNT         2274 a         4478 c         4573 g         1957 t
ORIGIN      
        1 tcaggccgag tccagaccta tcgcgacgat caggcgccga cattgaccgg cacggttacg
       61 ccgggtccgc gcaaccggga tgccgccggg agcctgtgcg tcaccaacac cgactatctg
      121 gccgaccgtg gcaagggtga aaacgtctat gtgaagatgt gccagcaggc tgacggcggc
      181 tgggcacccg ccgtgagctg aagccttgaa cgcgcgtttt cttgccggcg cccgccgtct
      241 gtgcggactg gccgtcctgc tggcggtcgc cccgcttccc gcggtggcac tcgacttcac
      301 gcctgtcaca ctcgacaacg gcctgaaact gctgatcgtt cacgacaagc gcccctgcgc
      361 ccggaatgaa tcctgcggtt tcacggcggg tgacgcgaac cgcctcaagg actttctcga
      421 acgggaccgc tacgtgcagg tcctgctcaa ctccaatggc ggcagttcca cggaaggcca
      481 gcgtgtcggc gctgtcctgc gcgcgcgtgg cgcatgggtc cgcgtcgcca agggacatca
      541 ctgcatcagc gcatgcaccg ccgcttttct gggcggggtg atccgcgagg tggatgaagg
      601 aggagagttt cacgtgcact cctcgtcgag ctacctgaat ggggtgtccg aagaggaatg
      661 gcagcgcgtt tcccgagacc ccgaccttga actcggcaac accttgcgcg ccaccgttgc
      721 agaccgggtc gaatggaccc tctcacgttt tgcctacgta cagcgcatga tcgccggcga
      781 gagtccgaag ggcctgctgg aaggcagcaa ctcggaatgt ctgagccgtg cccaggatca
      841 gctgctccag ccgggccgcc tgcgccaact ggcgcagcgg gtgcgtgtgg aaggtcgccc
      901 cgccctcaac gaaatcgaag aggcgaccga gcgccgtacc accgaattgt gcgtgggcat
      961 cctgaccgca caagccgacc gcctcggccc gcgggcgccc caggtgctgc gcatcatgga
     1021 catcatgacg gccagccgga tcggcgccac catcaacctc tctccccagg ccttgcgcga
     1081 agcgggcttc gtgacccaat gatgcgcctg aacagcacaa ctctgaacgt cctcatcgca
     1141 agcctgctgt gcggcggttg cgcctccacg agcgtgtcgg actccctgtg gacccaggtc
     1201 agtggcgacg gcaaccggat cgtgctgcga ctcgacccgg agcatccgcg cctgagacag
     1261 tttgcgagcg cgcagatcaa tctggccgcc gatttcgtcg acgacagcgg aacccccagg
     1321 acggcatccc tgggcggggg cagccgggta gacgcccaga cccgtgtcta tacgctcccc
     1381 gaaaccctga acaacccgcc gagcggcgag atctgcctgt acttctcccc cgcggatacc
     1441 cgcctcgggg cctttccgat ccgtgcgcgc acggccggtg gcaccgacaa cagcggcttt
     1501 cgatacccgg actgggaagc ccgcgttgga gcagaaacgc ggcggcgcct gaacgaccgc
     1561 cagaaatccg ggctcgacgc ccagcgcaat gcggcgcagc gtagcgtgca acaactccag
     1621 gccgaactgg acaaggatca gctggcgagc gtcgaggact gcgccaggcg gaccttcacg
     1681 gccaaggcgg atgcacgcgg gcgggacgtc atcgagcccg cggcccaggc ggcgaccgct
     1741 cggcgcatct gcgtgcgtcg gacccgtgag ctcaacctga ccagcaaatt cagcgttgtc
     1801 gatgccctgg gccagttccg caaccagttg ccgagcgaac gcagccgcca ggccggacag
     1861 ttcctgaccg actggcggaa ccacttcgag cgaacgggtg ccgattacgt gccggaactg
     1921 ggctcgcccc gcgaaccgct ggcaatcgcc acggatgccg agacactcgc cgcacgtccg
     1981 tcactccagc ccaaggattc gatcgccctg atcggcgcct ggctcgatgc ctacgatgtg
     2041 tgcgtccagg acaccacgca ccagctcggc caccgctacc agctctggca ggtcagccag
     2101 ggccgttcct cggaccgcgc cgccgcctat tccgactaca tggcacagga atgccggggg
     2161 aaattccgtc gtctgaatga acagcgcgcg ctcctcgcgg acgtcgaccg ccagcttggc
     2221 gccctgccat ccgcggcacc cacgtcgtca ccccaggccc agaaccgtaa cgtctcgctg
     2281 aacgcgctgg cctgcacgct gcccccccgc tgaccccgac gtcgcgcccc tgccgctcca
     2341 gctgacgtca tcagccaggc ggccgacccg gcctcagggt gcgggcttag cgctacgcgc
     2401 cacatcgccc cccgcattct tcgccagcag cacggcggcc agcagacgcc gggcggcgag
     2461 gtcgttggcg ctgcggcggg ccgtcagcag cgtggtctgg gcggtgacca cgttgaggta
     2521 gctgacggtg ccggcccgat actgattgag cgcgatggcc tccgcttcgg cggcggcggt
     2581 gaccgcggcg ttctgctcgg tcagttcgtg ctccagcaga cgcgcagcga ccagattgtc
     2641 ctccacctcc tgaaaggccg tgaggacggt ttgccgatag gtggcgaccg cctggtccca
     2701 gaccgccacg gcctggccgg tggcggcccg cttggcgcca ccgtcgaaga ggctggccgc
     2761 aacggtcggc cccagggacc agtagcggct ggacaggctc aggagttcgg acagatccga
     2821 tgagcggtag ccggccccgg cggtcagccc caataccgga aagcgcgccg cctgagcggc
     2881 ccccacggcc gcattggcgg cggcgacccg ccgctctgct gccgcgatat cgggacgccg
     2941 ctccagcaat gtggacggca ggctcaccgg ggtgacggcc accggcagcg gggcatcgct
     3001 ctcggccagc gtgaaggcgg ccggcgcctc gccgatcagg acggcgatgg cgtgctcgta
     3061 ctgggcacgg gacaggcgca catcgatggt ctgggcgcgg gtgctgcgca gcagggattc
     3121 cgcctggacc acgtcggcac gaccggcgac gccggcggcg taccggttgc gggtgagctc
     3181 gagggcgcgc tcgtaggctg cgacagtggc ctccagcaga cgcttctgac gctcggcgga
     3241 gcgaagctgg aaataggtct gggccagggt ggcacgggcc gacaggcggg ccgcggcgag
     3301 gtccgcgtcg gacgcctcga cacgcgcatc ggccgcttcc acgttgcggg cgatgcggcc
     3361 ccacacgtcg gcctcccagc tcgatgaagc gccgagggaa tagctgttgg tcaccgctgc
     3421 agggccctgg ctggcggtac tcgacacgct cgacccgctc gtgccgcggg tcatgccaac
     3481 gctgccggtg acggtcggaa acccgcttgc ccgggccgaa tcggcgaggg cacgggcctg
     3541 gcgatactgg gcctcgacgg cgcgcaggtt ctggttggag acgagcaggc gcgcctgcag
     3601 ctgatccagc accggatcgc cgaaggccgt ccaccaggcg tcacctgcgg ccggatcggc
     3661 cggcgcggcg ggcttccagt caccggcttc cttgtagacg gtgggcaccg ccatctcagg
     3721 acggacgtag tccgggccga tccgacaggc ggacagcaga acgacaagga tgacaggcag
     3781 ggggcgcagg caggtcttca tgacaggttt ccggccggag agcgggcaag gtcgggcacg
     3841 ggggcgcggg ccgcgccggc gcgcgatcgc agccggtcga gcagcacgaa caccacgggc
     3901 gtcgtgtaga gggtgaggat ctggctcagc accaggccgc cgacgatggc gatgcccagg
     3961 ggttgtcgca gctcggcgcc gtcgccggtg ccgatggcca ggggcacggc gccacagatg
     4021 gccgccaggg tggtcatcag gatcgggcgg aagcgcagca ggcaggcgtg gtggatggcc
     4081 gcgcgggcgc cgacgccttc gctacgctcc agcgcgaggg ccacgtcgat catcatgatg
     4141 gcgttcttct tgacgatgcc gatgaggagg atcacgccga tcatggcgat gaggctgaat
     4201 tccatgttga ccgccagcag ggcgagcagc gcgccgaccc ccgccgaggg cagcgtggag
     4261 aggatggtga ggggatggac gaggctctcg tagagcatgc ccagcacgat gtagatggag
     4321 atcagggcgg ccaggatgag cagcggctgg gtgctcatgg actgctggaa ggcgccggcc
     4381 gagccctgga agctggtgcg gatcgacacc ggtacgccga tctgctgcag ggcggcgtcg
     4441 atggcgcggg tggcctcgcc aagggtcttg ccctcgggca ggttgaagct gatggtggag
     4501 gcgaccgcac cgtcctggtg attgaccgac aggggcgcca gggtgctctc gaagcgcgcc
     4561 accgccgaca ggggtacggg ctggccgccg gacgccacca gggtgaggcg cgacagcacg
     4621 tccggatcct gcaggtaggc cggcgccgcc tccagcacca cccggtactg gttgagggga
     4681 ttgtagatgg tggacacctg gcgctggccg aaggcgttgt tgagggccgt ggtgaggctg
     4741 cgcatggtga ggccgtagcg ggtcatggcg tcccggtcta ccaccaggct ggtctgcggg
     4801 ccccggtcct gctggtcggt gttcacgtcg gtgagctcgt ccaggcggct catggcgttg
     4861 cggatgcgcg gctcccacag gcgcagatcg tccaggcggt cggcctgcag ggtgaactga
     4921 taggtggagt tgctgctgcg cccgccgatg cggatgtcct gcacgggatt gagatacagg
     4981 ttagcgccgg gaaagtgggc gagctggcgg cgcagacgcg cgaccaccgc atcggccgac
     5041 tcggcgcgta cgccgagggg tttgagggtg acgaacatgg aaccgtagtt gagctggaag
     5101 ccgccggtga agcccaccac gttgtccacc gcaggatcgg cgccgaccag ggcgagcaga
     5161 cgttccatct tgccgcgcat ggcctggaag gagatggcct ggtcggcctc cacgaagccg
     5221 cggatgcggc ctgtgtcctg ctgcgggaag aaacccttgg ggatggcgtt gtacagatac
     5281 acgttgcagc cgacggtgag caggagcacg gcaaggatca gccgcggatg atccagggcc
     5341 cagcccaggc tgtggcgata gccggccagc aggcgctcga aggcgccgcc gatccagcgg
     5401 tcgaagcgcc cccgtccggc cgccggcggc gtgggccgca gcaggcgcgc gcacatcatc
     5461 ggcgtggcgg tgagggacac caccatcgag accaggatgg ccgccgacag ggtcaccgcg
     5521 aactcccgga acagccgccc gacgatgccg cccatggcca ggatggggat gaacacggcg
     5581 atcagggaga ggctcatgga gatcaccgtg aaaccgatct cgcgcccacc gcgcagcgcc
     5641 gcacgcaggg gcgtgtgccc gtcctccaca tgacggttga tgttctccag cacgacgatg
     5701 gcgtcgtcca ccacgaagcc ggtggcgatg gtgagggcca tcagggacag gttgtccagg
     5761 ctgtagccgg ccagatacat gatggcgaag gtgccgatca gggacacggg cacgacgatg
     5821 ctgggaatca ccgtggcgcg cacgttgcgc aggaacagga agaccaccat caccaccagc
     5881 ccgacggaga tcaccagggt gcgctccact tcccgcaggg agccgcggat ggtgggcgtg
     5941 cggtccatca ccacatccag atccaccgcc gccggcacca gcgcgcgcag gtggggcagc
     6001 agcgcggtga cccgatccac cgtggcgatg atgttggcgc cgggctggcg gttgaggatc
     6061 agcagcaccg ctggccggcc gttggcggaa ccgaagttgc gctcgtcctg cacgctgtcg
     6121 cgcacccgcg ccacgtcgcc caggcgcacc gcagcgccgt tcttccaggt cacgatgagg
     6181 gggcggtatt ccctcgcggt gtaggcctgg tcgttggcgc cgatctgcca ggcccgctgc
     6241 ccgtcgtcga gcaccccctt gggccggttg gccaccgcac cgacgatggc cgtgcgcacg
     6301 tcgtccaggg cgatgccggc ggcggcgagg cggtccggat tcgcctccac ccgcaccgcc
     6361 ggcagggagc tgccgccgat gctcacctgg ccaatgccat ccacctgcgc caggcgctgg
     6421 gccagcaccg tggacgccac gtcgtagagc tggccctggg tgagggtggt cgaggtgagc
     6481 gccaggatca ggatcggcgt ctcggacgga ttcaccttac ggtaggtcgg gttgttgggc
     6541 aggctggtgg gaagctggcc gcgggcagcg ttgatggcgg cctgcacgtc acgggcggcc
     6601 ccgtcgatgt cgcggctcag atcaaactgg agggtgacgc ggctcgaccc gagggtgctg
     6661 gtggaggtca tctcgttgac cccggcgatc tgtccgagaa tgcgttccag cggcccggcc
     6721 acggtggcgg ccatggtctc cgggctggcg ccgggcagac tggcggagac cgagatggtg
     6781 gggtattcca cctggggcag cggcgacacc ggcagcaggc ggaaggcgat ggcccccgcc
     6841 agggcgatgg cgagggtcag gagcgaggtg gcgaccggcc ggaagatgaa cagccgcgac
     6901 aggttcatgg ggcggtcgcg gtgcggcgct gcagacgcag cgagagacgg tcgaaggcca
     6961 ggtagatgac cggcgtggtg aacagggtca gcacctggct caccatcagc ccgcccacca
     7021 gggtgatgcc cagcgggtgg cgcagctcgg agccgacgcc cgtgcccagc atcaggggca
     7081 gcgcacccag cagggccgcc agggtggtca tcaggatcgg ccggaagcgc agcaggcagg
     7141 cctggtagat cgcctcccgc gggcgcatgc cctggtggcg ctcggcctcc agggcgaagt
     7201 cgatcatcat gatcgcgttc ttcttgacga tgccgatgag cagcacgata ccgatcaccg
     7261 cggtgatgtc cagctcggca cgggccagca gcagcgcgag cagcgcgccc accgcggcag
     7321 agggcagcgt ggacaggatg gtgaccgggt gaatggtgct ctcgtagagc acgccaagca
     7381 cgatgtacat ggtcaccacc gcggcaagga tcagcagcag ggtgctggac agggaggact
     7441 ggaaggccag ggcggcgcct tcaaaaccgg tctcgatgct ggctggcagg cccagttcgg
     7501 cttcggcccg gcgaatcgcc tcgacggcat cgcccagggc cacgccgggc gccaggttga
     7561 aagacagggt cacggcgggg aattggccga tgtgattcac cgccagggtc gtcggccgct
     7621 ccgagacgcg ggcgacggcg ctgaggggca cctggacgcc cgccagggtg ggcacataga
     7681 gcttggcgag ggcttccggc cgggtacgga actcgggcag gacttccagc accacccgat
     7741 actggctggc ctgggtgaag atggtggaca cgaggcgctg gccgaaggcg ttgtagagca
     7801 cgttgtcgat ggccgcggtg gtcacgccga gccgcccggc cgcgtcccgg tcgatgtcca
     7861 cgtaggcctg gaggcccttg tcctgcagat cggaggccac gtcagcaagc tgtggcaagg
     7921 cgcgcagacg ctccaccagg cgcgggctcc attccgccag cgcggctgca tccggcgact
     7981 ggagcgagaa ctggtactgg gtgcggctga cccggtcctc gatggacagg tcctgcactg
     8041 gctgcatgta gagcgtgatg ccggccaccc gggcaagctc cggcgcgagc cggcggatca
     8101 cctcggaggc cgtgacgccc cgctctgcca ggggcttgag gctgatcagc aggcgcccgc
     8161 tgttgagggt ggcattggta ccgtccacgc cgatgaagga cgacaggctg gccaccgccg
     8221 gatcccggag gatgaccgcc gccaccgcct gctgacgctc ggccatggcg gcgaaggaga
     8281 tggtctgggg ggcctcggtg atgccctgaa tggcgccggt gtcctgcacc gggaagaagc
     8341 ccttgggcac cagaatgtag agcgcggcgg tgagacccag cgtggccagc gccaccagca
     8401 gggtcgcccc ctgacggtcc agcacccagg ccagcatgcg gccgtagcgg gcgatgaccc
     8461 ggtcgatgaa atcgcccgcg gcgcgggcga agcgcccctg ctgcgcctcg ggcacgtgcc
     8521 gcagcaggcg ggcgcacatc atgggcgtga gggtcaggga cacgaaggcg gagatcagga
     8581 tggcgatggc cagggtcacc gcgaattccc ggaacagacg tccgaccacg tcggccatga
     8641 agagcagcgg aatcagcacg gcaatcagcg acagggtcag ggagatgatg gtgaaaccga
     8701 tctgtcgcga gcccttgagg gccgcctcca tgggcgaatc gccctcctcc acgtaacggg
     8761 cgatgttctc gatcatcacg atggcgtcgt ccaccacaaa gccggtggag atggtgaggg
     8821 ccatcagcgt gaggttgttc aggctgaagc ccgccagata catgaccccg aaggtgccca
     8881 ccagggacag gggcacggcg acgctgggga tcagcgtggc cggcacgctg cgcagaaaca
     8941 ggaagatcac catcaccacc agggccactg ccagggccag ttccacctgc acgtcgtgca
     9001 ccgaggcacg gatggtggtg gtgcgatccg agagcacgcg cacgtcgagg gacgcgggca
     9061 ggctggcggt catctggggg atcagcgccc gcacccggtc ggccacctcg atcacgttgg
     9121 cgccgggctg gcgctgcacg ttgacgatga ccgcaccggt ggtgtcggcc cacgcggcaa
     9181 ggcgcaggtt ctccgcgtcg tccttgaggt tggccacgtc gtcgaggcgc accgggctgc
     9241 cgttccggta ggtgacgatg agcttgcggt agtcgtcggc cgagcgtagc tggtcgttgg
     9301 cgtcgatggt ggaggcccgc tgcgggccgt cgaagctgcc cttggcctgg gtgatgttgg
     9361 cgttggcgat ggcggtacgc acgtcctcca aggccaggcc gtaggccgcc agctgggtgg
     9421 ggttgacctg cacccgcacg gccggacgcc gcccgccggc gatgctcacc agaccgacgc
     9481 cgggcacctg ggcaatcttc tgggcgaggc ggctctccac aaggtcgtga acccgcgtca
     9541 ggggcagact gggcgaggag atggcaaggc tcaggatcgg cgtgtcggcc gggttcacct
     9601 tgctgtaggt gggcggcgcc ggcaggtcgg tggggagcag gttggaggcg gcgttgatgg
     9661 ctgcctggac ctgctgctca gccacgtcga gggtcaggtc cagggaaaaa cgcagggtga
     9721 tcaccgaggc gccgccggaa ctggtggagg acatttcgtc gagccccggc atctgcccga
     9781 actggcgctc cagcggcgcg gtgatggcgg aggtcatcac gtccgggctg gcgccgggat
     9841 agaaggtctt gacctggatg gtcgggtaat ccacctgggg cagggccgcg atgggcagca
     9901 ggcggtaggc caccagaccg gtgaggagga tcgccaccat cagcagcgcg gtggccaccg
     9961 ggcggacgat gaacagacgg gacggattca tcgccatggc gggtcagtgc gccggccggc
    10021 cgcctccggg gccacccccg ccgggaccgc ctttcggagg gcgcttgcgc cctggcgcat
    10081 tggcggcgtc gggtgcaatg gcttcgacgg cggcgccgtc acgcagcttg tcgaggccat
    10141 ccaccaccac cttgtcgccc aggtccagac cgccctccac ggcaatccgt tcaccgtcga
    10201 cagcgcccag cctgacggtg accgccgcca ccgtgttgtc cgcacccacc cgatagacat
    10261 agcgcccttt gtcgccctgc atcagggcgg cggtgggcag caccggcacc gccttgaggc
    10321 ggtccaggat caggcgcacg ttgacgaact ggttgggaaa caggctgcca tcctcattgg
    10381 cgaactccgc cttgagcttg agggtgccgg tggtggtgtc gatctggctg tcggcggcaa
    10441 tgagccggcc ggtggcgagc agggtgcgct gctcccggtc ccacgcctcg accgccagcg
    10501 gcttgccggc cttctggcgg gccatcactt cggcgacgcg ctcctcgggc accgcgaaga
    10561 cggcggtgat ggggctcacc tgggccagcg taaccacgcc ggtcgtatcc gacgcatgca
    10621 cgatgttgcc cggatccacc tggcgcagcc cggcccgccc ggcgatgggc gccgtgatgc
    10681 gggtatagga cagctgcagg cgggcgctgt ccacagcccc ctgatcggcc ttgaccgtgc
    10741 cttcgtactg acgcaccagc gcctcctggg tgtctacctg ctggcgggcg atggaatcct
    10801 gggcgagcag ccccttgtag cggtccagat cgatccgcgc gttctgaagc agggccttgt
    10861 cccgcagcag ctgtccctca gcctgggcca cggccacctc aaaggggcgc gcgtccacct
    10921 ccgccagcag atcgcccgcc ttcacggcct gtccttcccg gaacaccacc ttcatcagct
    10981 gaccgtccac ccgggcgcgc accgtcacct gattgcgcgg caccagactg cccagggccg
    11041 agacataaac gcggatgtcc cccgcctgta cctcaccgag aagcaccggc tgcgggcgat
    11101 caccccggtt acgtcctcga tcttcggcat ggaccttgct ttccgacatc gaggtgcgcc
    11161 atgcatacac gccgccgagc gcgagggcga gcaccaggaa ccagggccac aggcggcggc
    11221 ggggcggaga gacgggttgg tttgcggaag cggctgggga aacgtcggaa ggcgtgggcg
    11281 acatgaagat tttccggatt tcggcgcgcc ggacgagcgc agtcccggcg aatgcacaat
    11341 cctacacgat ggcgatgagt gacttccatc agactacggg acgttgcgag ggttcgggtc
    11401 gaacctgtcg gagcgcgctc cccgatggac gctccgacag gttcgacccg tccgtgaaga
    11461 aagtcgcagc cccgtgcatt cgcacggtat agaatgttaa atatcattgc tctacgttcg
    11521 caaaatgcaa tgcatttgtc atttactgcc ctgcaacaca ccgtttcaca ggttggccca
    11581 tgaaaatctc cttccgtcgg tccgcatgca ccattgcagc ggcgatcctc ctgcccgtca
    11641 cccacgccca gggttcgcct gacctcagcg ggctgctgtc gatcctcaac tcgacgccgg
    11701 aaggaacctg ggtccaggtc aacctgaaca gctacagtga agcctggacg cccgctgaat
    11761 tgcgtccgct ttatctgaca tccaaccccg acccgtcgaa gatcacgtac gcgtggccgt
    11821 ccttcgcgtg ggattcgaac cggggcgacc tcattctgta tggcggcggg cacgccaact
    11881 acagcggcaa cgacgtctac cgctggcatg gcaccacccg caagtgggaa cgcgccgctc
    11941 tgcccagtga actcctgttg aaccctcccg gcttctacac gcccattgac gggcctgacg
    12001 ccggaccgcc ttcgtcgcac acctacgaca acagcctgtt cctgcccatc atcgaccgct
    12061 tcatcgtctt cggcggcgcc gccttcaaca ccggcggccc ctacaaccgg atgaatcccg
    12121 atggcgtcac ctaccgcaac accggcccct acctgttcga cccctccaag gccgatccga
    12181 acaaagtcgg cggcaccacc ggctcccacg tcaagcgcgt cgccccgcac ccggaaatcg
    12241 tcggcggcaa catgtggacc aaccgggatt accgggccta caccttcacc ggcagcaccc
    12301 tgattcccaa cagccacatc aacggcgtca cggcatatgc gcaggaaaac ggtaaggacg
    12361 tcgtctactt cggcgcccgc cccggacagg gcaccgacca gcatctgttc aaatacacca
    12421 tctccagcct gaccgacgtc acccaggaca agctcgaaaa ggtcggccgc ttctggaatg
    12481 gcccatcgga ccagggcgca ggcacctacg atccgacccg caagctcttc gtccgcacgg
    12541 ggccggccac ccagccgatc acctattggg acctcaacaa cgccagcccg tccaacaacg
    12601 acgtggtcac cgtcccggtc gacgccagtg gcacctttga cttcaaccag atgcgcatga
    12661 cgggcatcga ctacgacccg accaccgacc agtatctgat ctggtacggc ggccccgatg
    12721 tctggcgcct gaccccgccg acgcccgtga cctccaccgg ctggaagctc gacaaggtca
    12781 gcaagaccag cagcgccgcg ccgacgatcg atgcggacag ccgcggcatc ctcggcaaat
    12841 ggaagttcat tcccaacctg aatgccttcc tgggcctgca ggatttcgtc aacggcaaca
    12901 tctggctgtt caaaccggtc ggctggaagc tccccaccgg taccggcaac ctgccgccca
    12961 ccgtcagcct caccagcccg agcgccggcg ccagctttgc cgtcggcagc atcgtttccg
    13021 tcaccgccac ggcatccgac tccgacggct ccgtgaccca ggtcgccttc tacgtcgatg
    13081 gcggcatcgc caccacgtcc tccggcccca cctacaccgc caacctcagt ggcctcaccg
    13141 ccggcaccca cagcctcacc gccgtggcca ccgacaatgc cggcgccacc accacctccg
    13201 cggccgtgag catcaccgtc accagcgcca ccaacacgcc gcccaccgtg agcctcacca
    13261 gccccgccgc cggtgccagc tt
//
LOCUS       DAOQPA010000099        13044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2458_length_13044_cov_18.247902, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000099 DAOQPA010000000
VERSION     DAOQPA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13044
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_13044_cov_18.247902"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2774)
                     /gene="pepN"
                     /locus_tag="PKX01_12950"
     CDS             complement(81..2774)
                     /gene="pepN"
                     /locus_tag="PKX01_12950"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HMZ77083.1"
                     /translation="MRTQQGTPILRSAYQPLAYGVESVELDFVLDPTATQVTSRLRCV
                     RHPDAAAGPIVLYGEALERVSLTVDGMAPAPGQLRDTASGLEIDVVGDVVNLEIVTRI
                     DPAANTTLSGLYQSRGGFFTQCEAEGFRRITYFPDRPDVMARFTVRLEADKATCPVLL
                     SNGNLLETGELPGGRHFARWEDPFPKPSYLFALVAADLQALERRVTTMSGREVLLQVW
                     VEARDLDRVEHAMNSLVHSMRWDEEVFGLELDLDRFMIVAVSDFNMGAMENKGLNIFN
                     TKYVLANPETGTDLDFENVESVVAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQQF
                     TADMMARAAESPAAAASARAVTRIANVRVLRNGQFPEDAGPMAHPIRPDSYQEINNFY
                     TATVYQKGAEVIRMIHTLLGREGFRNGMDLYFSYHDGQAVTCDDFVQCMAEANGADLD
                     QFMRWYAQAGTPRVKASGEYDAATGSYTLTLSQHTPATPGQPDKLPLHIPVMLGLIGP
                     DGRDLPLRLEGETRVGETGRLLHLRSDSQRWRFIGLGAEPVPSVLRGFSAPVILELDE
                     SDERLAFRMAHDSDPFNRWDAAQRYMERVILDLAAASADGRTMDVPQAFGHAFRALLI
                     DESLDPAFVAEALSLPSEPYLLERMAPADPEPLRAALIHLMRELGQAMQADFLSRYLD
                     LEVDGDYRYHPSDAGRRALRNLCLRYLMAWGGATAVELAVRQFEFAGNMTDRYGALAA
                     LVQSDSPRRAEALAAFHDRYRDDALVLDKWFALQAGAWRWSDAAPQTLDQVRTLMDHP
                     AFSLGNPNKVYALLGGYFRANPGEFHRADGLGYAFWADQVVILDRANPQVASRMARAL
                     EGWRKFTPAIQDGIRVALERVAGSAGLSPDVSEVVGKALKG"
     gene            complement(2810..3769)
                     /locus_tag="PKX01_12955"
     CDS             complement(2810..3769)
                     /locus_tag="PKX01_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ77084.1"
                     /translation="MMVAPERRRLRFRVPVRSLALLVIVIVGGGAYALLRGDGPAFLG
                     ALTRIPPGTWLSVAGLALISYGLRAVRWMGMLGHLGYSLPRGAGMTVYIAGFALTLTP
                     AKAGETIRAMYLLPWGVPVGASVAAFVAERLMDLVAVTGLSVLAGWGGQGFTWAVPVV
                     AAGGAAILMLVRSRLLPDVVGRIMGRAAGDALIQAVGPLHALLRLQVWMRWVGPTLLA
                     WGAQGVAFHLVVTGLGHAGPLPGLVGLYCLAILLGALSFMPGGLGATEAALVILLERH
                     GLGVADAVAAGLVTRGLTLWLAVLLGSLATARLAVVRSQASEG"
     gene            complement(3766..4182)
                     /locus_tag="PKX01_12960"
     CDS             complement(3766..4182)
                     /locus_tag="PKX01_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HMZ77085.1"
                     /translation="MDISYAATPFLAWLVAGGLKFCINSLRAGKPAFALIGYGGMPSN
                     HSAIVSSMAALIAFRQGIGDPAFGVALSLAFVVMLDANSLRRQVGRHAEAINRLTMTD
                     ARHVPLRERMGHTRAEILGGVVTGCVVAWVVSRLSA"
     gene            complement(4185..5072)
                     /locus_tag="PKX01_12965"
     CDS             complement(4185..5072)
                     /locus_tag="PKX01_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HMZ77086.1"
                     /translation="MHDSVAAVSLLGRIRGLLALMRPRQWVKNGFVMAPLVFSGAFGD
                     PLAARHAILAALLFCVASSATYIVNDMHDIERDRRHPKKSRTRPLASGIVTVPMALGL
                     LVLLYGVLVVGWFAMPAVLVVILAYLGLNVAYTFVLKHQPVVDIFTIAIGFVLRVYAG
                     AMALAVPVSSWMFVTTLCLALYLASVKRRQELVGSGSEGRKVLEKYSVGLVDRYAEMS
                     ATGALVFYSMFVMAARPQLVITVPLVLFGLFRYWFVVEQLDGGESPTDALLEDWQLLA
                     TLALWVGACMWVLWPAAGA"
     gene            5129..5530
                     /locus_tag="PKX01_12970"
     CDS             5129..5530
                     /locus_tag="PKX01_12970"
                     /inference="COORDINATES: protein motif:HMM:NF037976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMZ77087.1"
                     /translation="MKLALTYSILALIATCVNIGTQDLANRAYEGEFSLLLSIVAGTG
                     TGLIVKYILDKRYIFSFKARDAAHDGKTFLLYTLMGLITTVIFWGFEFGFDRLFQTRE
                     MRYLGGIVGLAIGYYAKYRLDKRFVFRSDPA"
     gene            5527..6825
                     /locus_tag="PKX01_12975"
     CDS             5527..6825
                     /locus_tag="PKX01_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ77088.1"
                     /translation="MSTPVHSWGRLSAAPHDVHSLGDRHRMAETLVRHTPGVAFGNGR
                     SYGDVGLNPGGHLWKTGQLDHFIEFDDASGLLRCEAGTLLRDIQQLAVPRGWILPVTP
                     GTQLVTVGGAIANDVHGKNHHVLGSFGDHVRSLTLVRTTGESIVCGPDVQPEWLAATV
                     GGLGLTGVIAEATLQLRRIASPWLDTETTPYGNLDEFFALADAAEARWEHTVSWIDCL
                     SGRGKGIFMSANPAPPQNREMPGAHRRSMPFTPPISLVNRLTLRPFNLAYYQLQKFKA
                     GRGVAHYEPFFYPLDNLLEWNRMYGPQGFYQFQSVVPRDVGRDAVAAMLQAIERSGKG
                     SFLAVLKTFGDRQAVGMLSFPRPGVTLALDFPNQGHATLKLLAGLDAIVREAGGRIYP
                     AKDARMPRDLFESGYPRLGEFLPFRDPGISSGLSRRLIGS"
     gene            6828..7571
                     /locus_tag="PKX01_12980"
     CDS             6828..7571
                     /locus_tag="PKX01_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ77089.1"
                     /translation="MQKIVIVGATSAMAEHCARLWAAQGPVDFTLVVRDAHKATAIAD
                     DLRVRSPGGRVRVLVADFLTPAGIRAAVDQAAAGTPIDIALIAHGSLPDQKACETDLS
                     ACNEALQVNGVSPALFAEAFAGTMLAAGRGTLALIGSVAGDRGRKSNYAYGAAKGLVT
                     RYAQGLQHRLAGSPIKVVLIKPGPTETPMTAHLAATGMRMAPVEDVARDIVAAVTAGA
                     PVAYTPGKWRIIMMVIRHLPAFIFNKMNI"
     gene            7883..8875
                     /locus_tag="PKX01_12985"
     CDS             7883..8875
                     /locus_tag="PKX01_12985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ77090.1"
                     /translation="MDTSDTPLLSIVIPCFNEEDVITSTVNRLRAFCATVKDVRVELI
                     FVDDGSQDGTRARLKSFGTEDPRIRIIGFARNFGHQVAVTAGIDAARGDAVVLIDADL
                     QDPPEVIHGMLTLWREGYDVVYGTRTDRPGESLFKRVTARLFYRVLSRLSDVPIPLDT
                     GDFRLMDRRVADTLRAMPERDRFIRGMVSWVGFRQTALPYQRAERLAGESKYPLRRML
                     RFATDGILSFSTKPLQISIALGMFAAFLSVLGVLYALYMRLFTHIWVEGWTALMIAIL
                     FLGGTQLISVGILGEYIGRIYAEIKQRPLYIVQEYWGFDDAEPVLSRSPVPGRR"
     gene            8878..9864
                     /locus_tag="PKX01_12990"
     CDS             8878..9864
                     /locus_tag="PKX01_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ77091.1"
                     /translation="MRRTSRPLLGLGLAGLFLWLILRQVDSGEMLRILGGARPDWTAL
                     AIAAFFCGYACRIERWRLLLCHDNPTLRWRHCAGPLLASVAANNVLPFRLGDLLRLFG
                     FNARLGIGTTTAFASLFQERLLDLLVLLIILGVLPVALGLDISRLMGAGTAVFLAGAS
                     LILGLLLRPTAIHPPVKIFVSFVTRWHASLGQRLDRESARVFTLLTQSAQPRLALRLL
                     MWSMLAWLIEGLVFYCCALALPAITQPAAAWLALPLGTLATLLPSSPGYLGTFDYFTA
                     WALSSAGNTATASATYAFFVHAILWGPVTLAGGCYVIARPVGVSARRQGNAP"
     gene            9861..11210
                     /locus_tag="PKX01_12995"
     CDS             9861..11210
                     /locus_tag="PKX01_12995"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ77092.1"
                     /translation="MNTAPIAIIGAGFTGLSIAYRLVQAGVPVTVLEAEPDIGGLAAA
                     FPVGGQTLDRFYHHWFTNDREVMTLIEELGLSDKVTVNPTNTGIYYAHQFFRLSTPLD
                     LLCFKPLGLLARLRLGLLALRARRVRDWHSLESLTAHEWLRALGGEDVYRVVWQPLLQ
                     GKFGPYADEVSAVWFWNKLKLRGGSRGKAGEERLAYFEGGFVALAEALARHIVAAGGR
                     IVRNARVTGVRPDGNQWQLETTAGQFRAHEVAATVALPLIADMIDTWAPADYLAGLRR
                     IRYLGNVCMVLELDRSLSDTYWLNVNDPSFPFVGIIEHTNFARPETYGGRHIVYLSKY
                     LPVTDTLFGLDADSFLAYALPYIQTMFPAFRREWVVAHHLWRARWSQPVVERFYSRLI
                     PPTQGPRQGFHVCSMAQIYPEDRGTNYALREGRRLAEAIIACRRTTSHSASFASEVP"
     gene            11207..12724
                     /locus_tag="PKX01_13000"
     CDS             11207..12724
                     /locus_tag="PKX01_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77093.1"
                     /translation="MKRFESVGLLHIAALLATVLTAYLPQLYEFVGTDIGVYYEIGRR
                     LATDGRLELWGNYWDHKPPLIYLYFAIFSHLDALLGDTSGLKLGALCAYSGLTLLAYR
                     ALSIARPTDNRILAALGSAAATSLVLGWLDPVANGILMVSALICAFSGLVLCADGKRD
                     TTVFLGGALIGLAPFLRPTALTALPFMLTLLFLEYRKTGERRRLWLALSGAVMVAGGC
                     ALWLLTFGGSAVFDSLIHYNADYGSRARSTTAVADFFFQDPIALCLAGVLLTSGVASL
                     SCATSPHRSISLVLWAWALTEVASIVFQRKIQSFYLFGFILPLLLAFTYSALNATQTF
                     RTIAAVLLSTILAGTLGYSAHKLDLRILQNPLVSQHTQKTNAIAQIMSAQNCKGVPND
                     VLVYGNRAQIYTHAAAVGIRPYSWTVFLNPVVDGSLFADRQRQLVDSLTANPASFIVY
                     VGGDGWSPNIGGRIEKIEALIKQRYTAIPVAQTGGLETPYNYQYRLYRKAECAEQ"
BASE COUNT         2461 a         4394 c         3808 g         2381 t
ORIGIN      
        1 ccccttgacg cgccgcaccg cccatgaaaa ggccgcccct cagggcggcc ttttcacttt
       61 cagacatcga aggaattaca tcagcccttc aacgcctttc cgaccacttc cgacacatcc
      121 ggcgaaagcc ctgcgcttcc cgccacccgc tccagtgcca cgcggatccc gtcctggatg
      181 gcgggcgtga attttcgcca gccttccagt gcccgcgcca tgcgggaggc tacctgggga
      241 ttggcacggt cgagaatcac cacctgatcg gcccagaagg cataacccag gccatctgcc
      301 cgatggaact ctccgggatt ggcgcggaaa taccccccca acaaggcata aaccttgttt
      361 ggattgccca aactgaaggc cggatgatcc atcagcgtac ggacctgatc cagagtctgc
      421 ggcgcggcgt ccgaccagcg ccatgcgcca gcctgcagcg caaaccattt gtcgagtacc
      481 agggcgtcgt cccgatagcg gtcatggaaa gcagccaggg cttcagcacg gcgcggagaa
      541 tcgctctgca ccagcgctgc cagggccccg tagcggtccg tcatgttgcc cgcaaactcg
      601 aactgccgca cggccagttc caccgccgtg gccccgcccc acgccatgag gtaacgcagg
      661 cacaggttgc gcagggcacg ccggccggcg tcggacgggt gatagcgata gtcaccgtcc
      721 acctccagat ccagatagcg ggacaggaaa tcagcctgca tggcctgacc cagctcacgc
      781 atcagatgga tcagcgcggc ccgcaggggc tcgggatcgg caggcgccat gcgctccagc
      841 aggtagggct cggagggcag gctgagggcc tccgcgacga atgccggatc gagggattcg
      901 tcgatcagca aggcgcggaa ggcatgtccg aaagcctgtg gcacgtccat cgtccggccg
      961 tcggcggacg cggcggccag atcgaggatg acacgttcca tatagcgctg cgcggcgtcc
     1021 cagcgattga agggatcgct gtcatgggcc atgcggaagg ccaggcgctc gtcggactcg
     1081 tcgagttcaa ggatcaccgg cgcggagaat ccccgcagca cggacggcac cggctctgcg
     1141 cccaacccga tgaagcgcca gcgctggctg tccgagcgca gatggagcag acggcccgtc
     1201 tcgccgaccc gcgtctcgcc ttcgaggcgc aggggcagat cgcggccatc cggaccaatc
     1261 agccccagca tcacgggaat gtgcaggggc agcttgtccg gctgcccggg cgtcgccggg
     1321 gtgtgctggg acagggtgag ggtgtaggac ccggtcgccg catcgtattc gcccgacgcc
     1381 ttgacccgcg gcgtgccggc ctgggcatac caacgcatga actggtcaag atccgcacca
     1441 ttggcctcag ccatgcactg cacgaagtcg tcgcaggtca cggcctggcc gtcgtggtac
     1501 gagaaataca gatccatgcc gttgcggaag ccttcgcggc ccagcagggt gtggatcatg
     1561 cggatcacct cggcgccctt ctggtaaacc gtggcggtgt agaagttgtt gatttcctgg
     1621 taactgtcgg gccggatcgg atgggccatc ggccccgcat cctcgggaaa ctggccatta
     1681 cgcaggacgc gcacattggc gatgcgcgtc accgcccgcg ccgacgcagc ggccgccggc
     1741 gactcggcgg cacgcgccat catgtcagcc gtaaactgct gatcgcggaa gacggtgagc
     1801 ccttccttca gggtgagctg gaaccagtcg cgacaggtga cccggttgcc ggtccagttg
     1861 tggaaatatt cgtgggcgac cacgctttcc acgttctcga aatccaggtc ggtgccggtc
     1921 tcgggattgg ccagcacata cttggtgttg aagatgttga ggcccttgtt ttccatggcc
     1981 cccatgttga agtccgacac cgcgacgatc atgaagcggt ccagatccag ctcgagcccg
     2041 aagacctcct cgtcccagcg catcgagtgc accagggaat tcatcgcatg ctcgacgcgg
     2101 tccaggtcac gggcctccac ccacacctgc agcagcacct cgcgcccgct catcgtggtc
     2161 acacgtcgtt ccagtgcctg cagatccgcc gccaccaggg cgaacaggta ggacggcttg
     2221 gggaagggat cctcccaacg ggcgaagtga cgaccgccgg gcagttcgcc ggtctcgagc
     2281 agattgccgt tggacagcag gaccggacat gtcgccttgt ccgcctccag ccgcacggtg
     2341 aagcgcgcca tcacgtccgg acggtcgggg aaatacgtga tgcggcggaa accttccgcc
     2401 tcgcactggg tgaaaaaacc tccgcgcgac tggtaaaggc ccgacagcgt ggtattggcg
     2461 gccggatcga tgcgggtgac gatctcgagg ttcaccacat cgcccaccac gtcaatttca
     2521 agcccggacg cggtgtcgcg cagctgcccc ggtgcgggcg ccataccgtc gaccgtcaag
     2581 gacacccgct ccagcgcctc gccgtacaaa acgatcggcc cggccgccgc gtcaggatga
     2641 cgtacacaac gcagacgact ggtgacctgc gttgcggtgg gatcgagcac gaaatcgagt
     2701 tcgaccgatt ccacgccata tgcgaggggt tgataggcag agcggaggat gggggtgcct
     2761 tgttgggtgc gcacagagga gtcctgatca gttcgtgtca gccggcattc tagccctccg
     2821 aggcctgact gcggaccacc gccagccggg ccgtggcgag actgcccaac agaaccgcca
     2881 gccagagcgt caacccacgg gtcacgagcc ccgcggcgac tgcgtcggcc acccccagtc
     2941 cgtgccgctc aagcaagatc acgagtgccg cctcggtcgc ccccagcccg ccaggcatga
     3001 aggacagcgc cccgagcagg atcgcaagac aatacagtcc caccagacca ggcagcgggc
     3061 ccgcatgccc caacccggtc accaccaggt ggaatgcgac cccttgcgcg ccccaggcca
     3121 gcagcgtcgg cccgacccag cgcatccaga cctgcaggcg caggagcgca tgcaacggcc
     3181 caacagcctg tatcaatgcg tcacccgcgg ctcgccccat gatgcgaccc acgacatcgg
     3241 gcaacaggcg cgatcgaaca agcatcagga tcgccgcgcc gcccgctgcg accacgggca
     3301 cggcccaggt gaatccctgc ccgccccacc ctgcaagcac cgacaatcct gtcacggcca
     3361 ccagatccat caggcgctct gcaacaaagg cagccacact ggccccgacc ggcacccccc
     3421 agggcagcag gtacatcgcg cggatcgttt cgcctgcctt ggcgggcgtc agggtaaggg
     3481 caaagccggc gatgtagacc gtcattccgg cgccacgggg caaagagtag cccagatgcc
     3541 ccagcatccc catccagcgg accgcacgca gtccataact gatcagcgcc agaccggcga
     3601 cactcagcca cgttcccggt ggaatacgcg tcaaggcccc gagaaacgcc gggccatctc
     3661 cccgcaggag tgcgtaagcg ccgccgccga cgatcacgat caccagcagg gccagactgc
     3721 gaacaggaac ccggaagcgc aaacgacgac gctcgggtgc gaccatcatg ccgacaggcg
     3781 actcaccacc caggccacca cgcacccggt cacgacgccc ccgagaatct ctgcccgggt
     3841 atggcccata cgctcccgta gcggcacgtg ccgtgcatcg gtcatggtaa gccgattgat
     3901 cgcttcggcg tggcgtccga cctggcggcg cagactgttg gcgtccagca tcacgacgaa
     3961 ggccaggctc agggccacac cgaacgctgg gtcgccaatg ccctgccgaa aggcgatcag
     4021 ggccgccatg ctgctgacga ttgcactgtg gttgctgggc atcccgccat agccgatcag
     4081 ggcaaaagca ggcttgccgg cccgcaggct gttgatgcag aacttcagac cgcccgccac
     4141 cagccaggcg agaaagggcg ttgccgcata cgagatatcc atggtcacgc cccagccgct
     4201 ggccacaaga cccacatgca ggcccccacc cacaaggcca gggttgccaa caactgccag
     4261 tcctcgagca gcgcatccgt cggagactca ccgccatcga gctgttcgac cacgaaccag
     4321 tatctgaaca gtccgaacag gaccagaggt acggtgatca ccagctgcgg acgggccgcc
     4381 atcacgaaca tgctgtagaa caccagcgca ccggtggccg acatctccgc gtatcgatcg
     4441 acgagcccaa cggaatactt ctcgagcacc ttgcgcccct cgctgccact cccgacaagc
     4501 tcctgacgac gcttgaccga cgcgaggtac agagccagac acagggtcgt gacgaacatc
     4561 cagctggaca cgggcaccgc aagcgccatg gcacccgcgt agacccgaag cacgaagccg
     4621 atggcaatgg tgaagatatc cacgacgggc tggtgcttga gcacaaaggt ataggccaca
     4681 ttgaggccga ggtaggccag gatgaccacc agcaccgccg gcatcgcgaa ccacccaacg
     4741 acgagcacac cgtacaacaa tacgagcagc cccagcgcca tgggtacggt gacgattccc
     4801 gaagccagcg gccgcgtgcg cgatttcttc ggatgccgac gatcccgctc gatgtcatgc
     4861 atgtcattga cgatgtaggt cgccgaagac gccacacaga acaggagggc tgcgaggatc
     4921 gcatgacgcg ccgccagcgg atcgccaaag gcacccgaaa acaccagtgg agccatgacg
     4981 aagccatttt tcacccactg acgcggccgc atgagggcca ggaggccacg gatgcggcct
     5041 aggagagaga ccgccgctac gctatcatgc atggatacca cgattcagaa tcaaaagacg
     5101 cgtcgttata ccacgcgcca ctcgcccgat gaaactcgcg ctgacctatt caatccttgc
     5161 gttgatcgcg acctgtgtaa acatcggcac ccaagatctt gccaaccggg cctatgaggg
     5221 ggagttttcc ctgctccttt cgatcgtggc gggcaccgga accggtctga tcgtcaagta
     5281 catacttgat aagcgttaca ttttttcgtt caaggcgcga gacgccgccc atgatggcaa
     5341 gaccttcctg ctctacaccc tgatgggtct gatcaccacc gtcattttct ggggtttcga
     5401 attcggattt gaccgcttat tccagactcg agagatgcgc tatttgggtg gaatcgtcgg
     5461 attggccatc gggtattacg ccaaatatcg gctcgacaag cgcttcgtat tccgttcgga
     5521 tcctgcatga gtacgcccgt tcattcgtgg gggcgtctgt cggcagcccc ccacgacgtg
     5581 catagcctgg gtgatcgtca ccggatggcc gaaacgctgg tccgccacac cccgggcgtg
     5641 gccttcggca acgggcgcag ctacggcgac gtgggcctga atcccggcgg ccacctctgg
     5701 aagacaggcc agctggatca tttcatcgag ttcgacgacg caagtggcct cctcagatgc
     5761 gaagccggca ccttgctgcg ggacattcag caactcgccg tgccgcgggg ctggattctt
     5821 ccggtcacgc cgggcaccca actggtcacg gtcggtggcg ccatcgccaa cgacgtgcat
     5881 ggcaagaatc accacgtcct gggatccttc ggcgatcacg tacgcagcct gaccctcgtc
     5941 cggaccaccg gtgaatccat cgtctgcggc ccggacgtgc agccggagtg gctcgctgcc
     6001 acggtgggcg gactgggcct gaccggcgtc attgccgagg ccacgcttca gctgcgccgt
     6061 atcgccagcc cctggctgga cacggaaacc accccttacg gcaacctgga cgagttcttc
     6121 gcactggccg acgccgccga ggcgcgctgg gagcacaccg tctcctggat cgactgtctt
     6181 tctggtcgtg gcaagggcat cttcatgagc gccaacccgg cgccacccca gaaccgcgag
     6241 atgcctggcg cccatcgtcg gagcatgccg ttcacgccgc cgatttcgct cgtcaacagg
     6301 cttaccctgc gccccttcaa cctggcgtat taccagctgc aaaagttcaa ggcgggtcgg
     6361 ggcgtcgccc attacgagcc attcttctat cccctcgaca accttctcga atggaacagg
     6421 atgtacggac cgcagggctt ctaccagttt cagagcgtcg ttccccgtga cgtcggtcgg
     6481 gacgccgtgg ctgccatgtt gcaggccatc gagcgctcgg gaaaaggctc tttcctcgcc
     6541 gtactgaaga ccttcggcga ccgtcaggcg gttggaatgc tgagctttcc ccgccctggc
     6601 gtgaccctcg ccctcgattt ccccaaccag ggacatgcca cgctgaaatt gcttgccggt
     6661 ctggacgcca tcgtccgcga ggccggcggc cgcatctacc ccgccaagga cgcccgcatg
     6721 ccccgcgacc tgttcgagtc cggctatcca cgtctgggcg aattcctgcc atttcgcgac
     6781 ccgggcatca gttcaggatt gtcccgccgc ctcatcggat cctgatcatg caaaaaatcg
     6841 tcattgtcgg cgcgacctcg gcgatggccg aacactgtgc ccgcctgtgg gccgcccagg
     6901 gtccggtcga cttcaccctg gtcgtccgcg acgcgcacaa ggccacggca atcgccgacg
     6961 acctccgggt acgcagtccg ggcggtcggg tacgcgttct cgtcgccgac ttcctcactc
     7021 cggcaggtat acgcgctgcg gtggatcagg cggcggccgg aacgccgatc gacatcgcgc
     7081 tgattgcgca cggatccctg ccggaccaga aagcctgtga aacggatctg tctgcctgca
     7141 atgaggccct tcaggtcaat ggcgtatcgc cggcactgtt tgccgaagcg tttgccggga
     7201 cgatgctggc agccggcaga ggcaccctgg ccttgatcgg ctcggtcgcc ggagaccggg
     7261 ggcgcaaatc caactacgcc tatggcgccg ccaagggcct cgtgacacgt tacgcccaag
     7321 gcctccagca tcgcctggca ggctcgccga tcaaggtcgt cctgatcaag cccggcccta
     7381 ccgaaacgcc catgacggcc cacctcgcgg ctacgggcat gcgcatggcg ccggtcgaag
     7441 acgtggcgcg cgacatcgtc gctgccgtga cggcaggcgc tcccgttgcg tacacacccg
     7501 gcaaatggcg gatcatcatg atggtcatcc gccatttgcc ggcgtttatc tttaacaaga
     7561 tgaacatctg agcacactgc aagcgactgg atcggctcga acccgcgccg atcagacggc
     7621 gccaccgata gccctctaaa tgcagcgtct tctcggctga tatacttttt caatcgttga
     7681 acggacactc cccacaaggt cacacccgat tttcaggccg agtcccatcg cacgaccggc
     7741 gatccagcag cgggcatcac ccgatatgtc gcccagggcg tctgcttgct gacgggctgg
     7801 agatgtttct cggaaatctc ttccgaagct ggtccgcccc gcccccatca cacgcttccc
     7861 gctggccacc atgacgacgc aaatggacac atccgacacg ccgctcctgt ccattgtcat
     7921 tccgtgcttc aacgaagagg acgtcattac cagtaccgtg aatcgtctca gagccttctg
     7981 cgcgacggtc aaggacgtcc gggtggaatt gatctttgtc gatgacggca gtcaggatgg
     8041 cacccgggcc cgtctgaaat cattcgggac agaagatcca cgtatccgga tcatcggttt
     8101 tgcacgtaat ttcgggcacc aggtggctgt gacggcgggt attgacgcgg cacggggcga
     8161 cgccgtggta ctcatcgatg ccgacctcca ggacccgccg gaggtcattc acgggatgct
     8221 gaccctctgg cgggagggct acgatgtcgt ttacggcacg cgcaccgatc gccctggtga
     8281 atccttgttc aagcgcgtga cggcccgact gttctaccga gtgctgagcc gattgtctga
     8341 cgttccgatt cccctcgaca cgggggactt ccggctgatg gatcgacgcg tggccgacac
     8401 cttgcgggca atgccggagc gcgatcgctt catccgtggc atggtcagct gggtcggatt
     8461 tcgccagaca gcgctaccct accagcgcgc cgagcgactc gccggagaga gcaaataccc
     8521 gctgcgaagg atgctccgat tcgccactga cggcattctg tccttttcga ccaaaccctt
     8581 gcagatctcc atcgccctcg ggatgtttgc tgccttcctg tccgttctgg gtgtgctcta
     8641 cgccctgtac atgcgcctct tcacccacat ctgggtggag ggctggacgg ccctgatgat
     8701 tgcgatcctg tttcttgggg gaacacagct catcagcgtg ggcatccttg gcgagtacat
     8761 tggccggatc tacgcggaga tcaagcagcg ccccctctac atcgttcagg agtactgggg
     8821 ctttgacgat gcggaacccg tgctgtcgcg cagtcctgtc ccggggcgcc gctgagcatg
     8881 cggcgaacct cgcgtcccct gctcggactg ggtctcgccg ggctcttcct gtggctgatc
     8941 ctgcggcagg tcgattcggg cgaaatgctc cggattcttg gcggagcacg cccggactgg
     9001 acagccctcg cgatcgcagc gtttttctgc ggttacgcct gccggatcga gcgctggcgc
     9061 ctgttgctgt gtcacgacaa ccccaccctg cgctggcggc attgcgcggg ccccttgttg
     9121 gccagcgtgg cagccaacaa tgtgctgccc tttcgcctcg gtgatctgct gcgtctgttc
     9181 ggtttcaatg cccgtctggg catcgggacg accaccgcct ttgcaagcct gtttcaggag
     9241 cgcctgctgg atttgctcgt gcttttgatc attctcgggg tgttgcctgt cgcgctgggt
     9301 ctggatattt cccgtctgat gggtgcaggc actgcggtct ttcttgcagg cgcgagcctg
     9361 attcttggcc tgctgctgcg tccgacggcg atccatcctc ccgtcaagat cttcgtcagc
     9421 ttcgtcaccc gctggcatgc ttcactcggg cagcggcttg accgggaatc cgcacgggtc
     9481 ttcacgcttc tgacacagtc cgcgcaaccc cggctcgcgc ttcgtttgct gatgtggtcc
     9541 atgctcgcct ggctgatcga aggactggtc ttctactgct gtgcgctagc gctcccggcc
     9601 atcacccaac cggcggcagc ctggctcgcg ctcccgcttg gcacgcttgc gaccttgctg
     9661 cccagttcgc ccggctatct gggcacgttc gactatttca ccgcctgggc actttcaagt
     9721 gccggcaata ccgcgaccgc atccgcaacc tacgccttct tcgtgcatgc catcctgtgg
     9781 ggtccggtga ccctcgcggg gggatgctac gtgatcgcac ggcctgtcgg tgtcagcgcc
     9841 aggcgccagg gaaatgcacc atgaacaccg cgcccatcgc cattatcggg gcaggcttca
     9901 ccggcctgtc catcgcctac cgtctggtac aggcaggcgt gcccgtcacg gtcctcgaag
     9961 ccgagccgga catcggcggg ctcgcggccg ctttccctgt tggcgggcag acgctggacc
    10021 gtttctatca tcactggttc accaatgacc gggaggtcat gaccctgatc gaagaacttg
    10081 ggctcagcga caaagtcacc gtcaatccaa ccaataccgg gatctattac gcccaccagt
    10141 tcttccgcct gtctactcca ctggatctcc tgtgcttcaa gccgctgggt ctgctcgcgc
    10201 gcctgcgcct ggggctgctc gcgttacggg cacgcagagt cagggactgg cacagcctgg
    10261 agtcattgac tgcccatgag tggcttcgtg ccttgggtgg cgaggacgtc tatcgcgtgg
    10321 tctggcaacc cctgcttcaa gggaagttcg gcccctacgc cgacgaggtg tcggcggtat
    10381 ggttctggaa caagctcaag cttcgcggcg gcagtcgcgg caaggccggg gaagaacgac
    10441 tcgcgtattt cgaagggggt ttcgttgcac tggccgaggc gcttgccagg cacatcgtcg
    10501 cagcaggcgg acggattgtc cggaatgcca gggtgaccgg cgttcgtccg gacggaaacc
    10561 aatggcagct cgagaccacg gcaggccagt tccgggccca cgaagtcgcc gccaccgtgg
    10621 cgcttcccct gatcgccgac atgatcgaca catgggcccc cgcagactat ctggcaggcc
    10681 tgcggcgaat ccgctatctc ggcaacgtgt gcatggtgct cgaactcgat cgctccttgt
    10741 cagacaccta ctggctgaac gtcaacgacc ccagctttcc cttcgtcggc atcattgaac
    10801 ataccaactt cgcacgaccg gaaacctacg gcgggcggca catcgtctat ctttccaagt
    10861 atctgcccgt caccgacacc ctgtttggtc tggacgcaga cagtttcctg gcgtacgccc
    10921 taccctacat ccagacgatg tttccggcat ttcgccgcga atgggtcgtg gctcaccatc
    10981 tatggcgggc acgctggtcc caacccgttg ttgaacgctt ttacagcagg ctcattccgc
    11041 caacccaagg tccccggcag ggatttcacg tctgctccat ggcacagatc tatcccgaag
    11101 accgtggaac caattacgca ctgcgggaag gccgccgcct ggccgaagca atcattgcat
    11161 gccggcgcac cacatcgcat tcagcctcat tcgcaagcga agtcccatga aacgattcga
    11221 atccgtcggc ctcctgcaca tcgcggcttt gctggcgact gtcctgacag cctacctgcc
    11281 acaactgtat gagtttgtcg gaaccgacat cggcgtttat tacgagatcg gccgacgtct
    11341 cgcaacggac ggacgacttg aactctgggg aaattactgg gatcacaagc cccccctcat
    11401 ctatctctac ttcgctattt tcagccatct ggacgcattg ctcggcgata cgtccggatt
    11461 gaagttaggc gcgttgtgcg cgtactccgg attgacgctt ctcgcctatc gcgctctttc
    11521 catcgcacgg cccaccgaca accgcatcct cgccgccctg gggtctgctg cagccaccag
    11581 cctggtcctc ggctggcttg atcctgtggc aaacgggatc ctgatggtca gtgccctgat
    11641 atgtgcattt tcggggctcg tactctgcgc cgatggcaaa cgcgacacca ctgtatttct
    11701 gggtggcgca ctgattgggc tggcgccttt cctgcgaccc accgcgctga ccgcattgcc
    11761 tttcatgctc acgctgcttt ttctcgaata tcggaaaacc ggcgaacggc gtagactctg
    11821 gctggcgctc tcgggcgcag tcatggtggc cggaggatgt gctctttggc ttctgacatt
    11881 cggcggctcg gcggtattcg acagtctcat tcactacaac gcagactacg gaagccgcgc
    11941 acgcagcaca accgccgtag cggatttctt ttttcaggac ccgattgccc tgtgcctggc
    12001 tggagtcctc ctgaccagcg gcgtcgcatc cttatcctgc gcaacatcgc cccatcgttc
    12061 gatcagcctc gtcctgtggg catgggcact cacggaagtg gcttccatcg tatttcaacg
    12121 aaaaatccag tcgttttatc tctttggctt catcctgcct ttgctgctcg ccttcaccta
    12181 ctctgcatta aatgcgacac agaccttccg cacaatcgcc gcggtactgc tctccacgat
    12241 acttgctgga acgctcggat atagcgccca caaattggat ctgcgcattt tacagaaccc
    12301 tctcgtcagc cagcacactc agaaaacgaa cgccatcgcc caaatcatga gtgcacagaa
    12361 ttgcaagggc gtgccaaatg acgtactcgt ctatggtaat cgcgctcaga tctataccca
    12421 cgcagcggcc gtcggcatcc gcccctattc ctggacggtc tttctcaacc cagtcgtgga
    12481 cggaagcctt ttcgcggatc gtcaacgtca actcgtggac tcactgacag ccaacccggc
    12541 gtccttcatt gtttacgtgg gcggcgacgg atggtccccc aacatcggcg ggcgtattga
    12601 gaaaattgaa gcattgatta aacaacgcta cacagcaatc cccgttgcac aaacaggcgg
    12661 gctggaaaca ccctacaact atcaataccg cctgtacagg aaagccgagt gcgctgaaca
    12721 gtgaagttag cagccccccg ccaagcggcc gggcagcgtt gccactgacc acccgcccca
    12781 gcatcccaac ccacacgcag agcgcaaaaa gagccttcag ccccaagccg gccaccgccc
    12841 gcagcagcca gcgcacgttg taacccgtcg gtcctgaaca atcagcccgc ccctgtcccg
    12901 gcgcgggctg aacgctcctt caacttcaac ttcacaacgc cggtggatcg atgccccgct
    12961 cgcgcatcca gcgccgcagc tggttggcgt tgatcccgtt cgccagcgcg acacccgcga
    13021 ccgacgcgcc gggctccccg cagg
//
LOCUS       DAOQPA010000100        12902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2494_length_12902_cov_18.791391, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000100 DAOQPA010000000
VERSION     DAOQPA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_12902_cov_18.791391"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKX01_13005"
     CDS             complement(<1..905)
                     /locus_tag="PKX01_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77094.1"
                     /translation="MEEFIGGLWHRFITRAASRSHPEAAVRLSEIERTAGILFRALGG
                     DSGLRVAAAAEVEHGARRSWLARIAHAGEKVAHAARDDETLRLPAEIALFPERRLNRD
                     LYLWLIAQGAALPELPGNWIVANQTASAATLARFPGLAARYRRLVAAVLAERPDPARL
                     PADEAAAEAAIRQALTDPGSVMTLPPARRPPQPVPLWLYPAPVAVDRRAAPGPATPAE
                     RRPGETRPQAAHRARHHRQRHAHHHALVPRRRDAQPAQDGGVVPGQGRPLADLPMVST
                     RPAAHGRRLGAGRQRRTGRPARARGG"
     gene            complement(914..1405)
                     /locus_tag="PKX01_13010"
     CDS             complement(914..1405)
                     /locus_tag="PKX01_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77095.1"
                     /translation="MSGLNDFALARALHVLAVLIWIGGVAFVTWVLLPACRDLAEPAA
                     RIDLFERLEHRFAAIARAAVLLAGASGLYLVWRLDLWSRFAQPAEWWWMHAMVAVWTV
                     FALMLFVLEPFVLHKLFRQLAQADPDATFARIRRLHGVLLAASLVTVLGAVAGSHGGW
                     IFG"
     gene            complement(1402..2400)
                     /locus_tag="PKX01_13015"
     CDS             complement(1402..2400)
                     /locus_tag="PKX01_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ77096.1"
                     /translation="MNPARVIPIHAVPATQPDGGRQAQRRLAQGAFFVLFVLAPVFDL
                     LRYDLVAGHAWFLGMPWHAGLEDFSAGRIGALEAGLRVLVRVLLPILAAGAVLIGVAW
                     RWGRLYCGWLCPHFSVVETINGLMRRASGKPSVWEPHALPARAPDGSPLPVDPRWWGA
                     TVPLAVAFAFVWAVVLLTYLLPPFQVYGHLLAGTPTRNEALFIGAATLVLSAEFLFAR
                     HLFCRFACAVGMFQSVAWMGNRDAMVVGFERARAAQCVGCDAACEHVCPMRLKPRAIK
                     RAMFTCTQCAQCVSACATTQRDHPSGPLLHWVSGAAARHNEAAFRAPRAEEWRKHP"
     gene            complement(2397..2798)
                     /locus_tag="PKX01_13020"
     CDS             complement(2397..2798)
                     /locus_tag="PKX01_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77097.1"
                     /translation="MLPVDPADADVAGRELAVAAEALYLANLMLIPGLGFIVLAVLWS
                     LRHRDAPPLARSHLRQTMAASLWAVALLIVANGAILLLGGYRAASTWVVLILYFTLVH
                     SSLILCGAVGLSRALAGRSFRFPVVGPREVA"
     gene            complement(2830..3615)
                     /locus_tag="PKX01_13025"
     CDS             complement(2830..3615)
                     /locus_tag="PKX01_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HMZ77098.1"
                     /translation="MTANLPFYEAAGNEIQVFEQAWKNRLPLLIKGPTGVGKTRFVAH
                     MAARLGLPLFTVACHDDLTAADLVGRHLLGDGQTVWCDGPLTRAVRQGGICYLDEVVE
                     ARRDTTVVIHPLADDRRVLPIERTGETLHAPDAFMLVMSYNPGYQNFLKGLKPSTRQR
                     FVSLRFEFPAAAREEAIVIGETGCDPMLAKRLVSIAGRLRALKDVDLEEVVSTRLLVY
                     AAKLVKVGLDPVEACRSALVESLTDDADVADALLEVVQATFGR"
     gene            complement(3624..3884)
                     /locus_tag="PKX01_13030"
     CDS             complement(3624..3884)
                     /locus_tag="PKX01_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMZ77099.1"
                     /translation="MNVRQLNFAWVVLAVATALTWELGEHGAAGPAAMAALLAISFVK
                     GRLVVLDFMGLRGVKFFWRALLLGWLLVVAGLIALAYWIALR"
     gene            complement(3881..4462)
                     /locus_tag="PKX01_13035"
     CDS             complement(3881..4462)
                     /locus_tag="PKX01_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HMZ77100.1"
                     /translation="MSTSARPAPAAAPPGDLAIWFFILAELLAFGVFFAAYAFARARH
                     PDLFDAMQATLDRRAGAVNTLLLITGSFCVARAVEAAGRDRSATAARWLAGGLVCGAG
                     FLGVKGVEYAAKFGAGINLSTNTFYMFYLSLTFFHFMHVILGLVILAVLWVQTRRGAY
                     GAGRMNGLESGAAYWHMVDLVWIVLFPLVYVMR"
     gene            4631..5569
                     /locus_tag="PKX01_13040"
     CDS             4631..5569
                     /locus_tag="PKX01_13040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77101.1"
                     /translation="MKILLVDDSRAVAAVMGARLSGAGYEVILAENGKVAVEQFRASA
                     PDLVLMDIEMPVMNGFQAAQAIRELEATGEWAWTPIIFLTASDTPENLVTAIEAGGDD
                     FIAKILPEAVLEAKMKAMARIARLRDQLSAANRKLTEQASQDGLTGLCNRRYMDLRLD
                     SQWAGCCDAREPFGLLMLDVDHFKKYNDHYGHQAGDDCLKAVARAISETVEAANVRGD
                     VHGALAARYGGEEFAVLMPRCTRDTFRALADAICTAIRTLAIPHERNAEWGIVTASVG
                     GAYAAQAEGRVALRFREADANLYRAKDLGRNRAVLD"
     gene            complement(5586..6077)
                     /locus_tag="PKX01_13045"
     CDS             complement(5586..6077)
                     /locus_tag="PKX01_13045"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ77102.1"
                     /translation="MNGPTVRWEGLRGEAYVMIQVALLVMLVFGPRTVAGLPPWPAEA
                     ALPALIGGIALMAAGGLIAALGALRLGSNLTPLPHPKDGATLVVSGLYARVRHPIYLG
                     LILSAFGWALFHQGWLTLGWAALLFVLLDVKSRREERWLADRFPAYADYRRRTRRLIP
                     WLY"
     gene            complement(6074..7153)
                     /locus_tag="PKX01_13050"
     CDS             complement(6074..7153)
                     /locus_tag="PKX01_13050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ77103.1"
                     /translation="MTLAEAARGRDNNFHLIRFLAALAVLYGHCYPLAGGGAPPLADW
                     LGIDIGSVAVDVFFVTSGFLVTASLRDRPGVRAFVWARVLRIYPALWGVVLLTVAVLG
                     PLLTTRPAAAYWADPGVVDYLIKCASLWGGIAIHLPGVFEANPYPAAVNGSLWTLPYE
                     LRMYAILALLGIVVQRLGRRRQGVLTGAVALIAGVAGGTMLVRHFFHTDAGYFARFAW
                     MFFCGACLQMAAARVPLGWSGLLAGGGVLALASLQRDVFFVAYVLLLGYLVVALAHVP
                     GGVIRRFNRVGDCSYGVYLYAFPVQQTVVALLPGITPVGLLAVSAPLTVALAGLSWIC
                     VERRALAFKGRMDVERGMATEGSMR"
     gene            7337..7828
                     /locus_tag="PKX01_13055"
     CDS             7337..7828
                     /locus_tag="PKX01_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77104.1"
                     /translation="MGAIFAKTRKGQQEIETRSGGLHPRVRRTLIVVDGKRSVDELRE
                     ALRSDDLQHTLGLLEEEGYIELISGARLAEAAPASISAFRLHPPGEDPLRLQQSRNFM
                     LNTLRTFVGAVGTSSLQDRLEAARTHADLRALFDEWYHALVTSRDGRREAEHLRSKLL
                     EIL"
     gene            complement(7840..8175)
                     /locus_tag="PKX01_13060"
     CDS             complement(7840..8175)
                     /locus_tag="PKX01_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HMZ77105.1"
                     /translation="MAFDEGLAERLREAFAGMTDVGERRMFGGLALMWRGHMCVGIVG
                     ETLMVRVGPDAYAAALARPHARPMDFTGRPLRGYVYVAPDGFAEDADLAAWVEAARLY
                     VAGLPPKGS"
     gene            8279..8581
                     /locus_tag="PKX01_13065"
     CDS             8279..8581
                     /locus_tag="PKX01_13065"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HMZ77106.1"
                     /translation="MFKKLSALYTLIREDLRILGFALRHPARPVWLLPALVALGLYVL
                     SPIDLLPDLVPVLGWVDDLILVPLVIAWLVSRLPVDLRRAWQDARRTVDAPAVRRA"
     gene            complement(8586..8918)
                     /locus_tag="PKX01_13070"
     CDS             complement(8586..8918)
                     /locus_tag="PKX01_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HMZ77107.1"
                     /translation="MDWLLLFVAIVAEVIATSALKLADGFRRLWPWGIVVSCYSLALY
                     LLSLTLDVLPVGVVYAVWSGSGVALMTLVGRYFFGQKLDRPALIGIGLIVAGVVVLQL
                     FSAAVVRA"
     gene            complement(8937..9320)
                     /locus_tag="PKX01_13075"
     CDS             complement(8937..9320)
                     /locus_tag="PKX01_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HMZ77108.1"
                     /translation="MAAPDPTATPAPAPRAGTLQWVLLFVAIVAEVIATSTLKATEGF
                     TRLWPSVVVVVCYETAFILLSLSLKKIPVGIVYAVWSGVGVALITVVAWVFLDQTLDP
                     AGLLGIGLIVAGVVVINAFSETVTE"
     gene            complement(9320..9532)
                     /locus_tag="PKX01_13080"
     CDS             complement(9320..9532)
                     /locus_tag="PKX01_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77109.1"
                     /translation="MRAFVLAGLLATGLAACGFGDAGSSAATAAKLKAQEAQQAEAVK
                     AQVESQLEAARLQAEQRRRDIEAATR"
     gene            complement(9529..10644)
                     /locus_tag="PKX01_13085"
     CDS             complement(9529..10644)
                     /locus_tag="PKX01_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HMZ77110.1"
                     /translation="MSPPAAVSALFQPVRLGDLDLANRIVMAPLTRCRVTNGEAAPDA
                     TQAQYYAQRAGAGLIVSEGTIVSPQGRGYPFTPGLWSAAQVAGWRLVTDAVHAAGGRI
                     VCQLWHCGRLSLPEFHGGAAPVAPSAIDPQWKMFGSAGLTPTVTPRALTREEIAGIVA
                     DFARAAAHAREAGFDGVEIHSSNGYLFHQFFARCSNVRDDEYGGSHANRARLLFEVLD
                     AVSRELPLGRVGIRLNPMMNTLHGIVVDEDTVPFFDDLVRTADGLGLAYLHLTEPFQP
                     GQLDGVARPLDAVAAHFRAFTRTPILVNGGLSPQRGAELVAQGTADGAVFGRLFISNP
                     DLPARLQAGAPLAEWDMDTFYQGGEKGYLDYPRWEAA"
     gene            10834..11343
                     /gene="napF"
                     /locus_tag="PKX01_13090"
     CDS             10834..11343
                     /gene="napF"
                     /locus_tag="PKX01_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HMZ77111.1"
                     /translation="MDITRRRFLGGRIGRNPGPAPFRPPWSLPEAAFLNQCTRCDDCV
                     KACPTGLLRRADDGFPVADFSAAECTFCGDCVPVCPPGALVRVGDAAPWSFGVRIGEG
                     CLALQQVECRICGEVCDPRAIRFRPRPGGIAPPEVDNTSCTGCGACIAPCPVVAVTRI
                     PLTTAPEAP"
     gene            11340..11630
                     /locus_tag="PKX01_13095"
     CDS             11340..11630
                     /locus_tag="PKX01_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HMZ77112.1"
                     /translation="MNIVSLVLRIHPDTRAEAEAALTAFPGVECHGMSDDGKLVVTVE
                     DAEGASMSDTLIALHRVPQVLAATLAYEYTDRTAATPTHAPNSNSPCEEAQS"
     gene            11627..>12902
                     /locus_tag="PKX01_13100"
     CDS             11627..>12902
                     /locus_tag="PKX01_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ77113.1"
                     /translation="MTINRRDFIKAQAIATAAATAGISLPVVAAPKEAPAAAGGDTRV
                     RWDKAACRFCGTGCSVLVGVQDGRVVATQGDPDAPVNRGLNCIKGYFLSKIMYGEDRL
                     TKPLLRMKDGKYDKNGEFTPISWNQAFDIMAEKWKAALKAEGPGSIGMFGSGQWTIWE
                     GYAASKLMKAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEQADAFVLWG
                     SNMAEMHPILWTRITDRRLTKTDVQVHVLSTFEHRSFELADNGMIFVPQTDLAILNYI
                     CNYIIQNNKVNQEFVKKNVNFKMGETDIGFGLRPAHALEKDAKFNGYPGADGKPKNNP
                     NDAKPITFDEFKKFVSTYTLDYTTKLTGVPAERLKKLAELYADPKKKVVSFWTMGFNQ
                     HTRGTWVNNMIYNVHLLVGKISTPGSGPFSLTG"
BASE COUNT         2278 a         4610 c         4307 g         1707 t
ORIGIN      
        1 ccgcctcggg cacgcgctgg ccttccggtg cgccgctgcc ggccggcgcc gagccggcgt
       61 ccatgcgcag cagggcgggt ggagaccatt ggtagatctg ccagcggtcg accttgccct
      121 ggaacgacgc caccatcttg tgcaggttgg gcatctcgtc gtcgaggtac cagcgcgtga
      181 tggtgagcgt gccgttgccg gtggtgtcgc gctcgatgcg cggcttgagg tcgggtctcg
      241 ccgggacggc gctcggccgg cgtggccggg cccggcgcgg cgcggcgatc caccgccacc
      301 ggtgcgggat acagccacag gggcacgggc tggggcggtc ggcgggcagg gggcagggtc
      361 atcacgctgc cggggtcggt gagggcctgg cggatggcgg cttcggcggc ggcctcatcg
      421 gcgggcagcc gcgccgggtc ggggcgctcg gcgagcaccg ccgccaccag ccggcggtag
      481 cgcgcggcca ggccggggaa gcgcgccaga gtggcggcgc tggcggtctg gttggcgacg
      541 atccagttgc ccggcagttc gggcagcgcg gcgccctggg cgatcagcca caggtacagg
      601 tcgcggttga ggcggcgctc cgggaacagg gcgatctccg ccggcaggcg cagggtttcg
      661 tcgtcgcggg ccgcgtgggc gaccttttca cccgcgtggg cgatgcgcgc cagccagctg
      721 cgccgggcgc catgctccac ctcggcggcg gccgccacac gcaggccgga atcgccgccc
      781 agggcgcgga acaggatgcc cgcggtgcgt tcgatctcgc tgagcctgac cgctgcctcg
      841 ggatggctgc gggacgcggc gcgggtgatg aagcggtgcc atagcccgcc gatgaactct
      901 tccatgaccc gtcctatccg aagatccacc cgccatgact gcccgccacc gcgcccagca
      961 cggtgaccag gctggccgcg aggagcaccc catgcagccg ccggatgcgc gcgaaggtgg
     1021 cgtccgggtc ggcctgggcg agctggcgga acagcttgtg gagcacgaag ggctccagca
     1081 cgaagagcat cagcgcgaag accgtccaca ccgccaccat ggcgtgcatc caccaccact
     1141 cggcgggctg ggcgaagcgg ctccacagat cgagccgcca caccaggtag aggccggacg
     1201 cgcccgccag cagcaccgca gcgcgggcga tggcggcgaa acgatgctcc agacgctcga
     1261 acaggtcgat gcgcgcggcc ggctcggcca gatcgcggca ggccggcagc agcacccagg
     1321 tgacgaaggc gacgccgccg atccagatca gcacggccag cacgtgcagg gcgcgggcca
     1381 gggcaaagtc gtttaaaccg ctcacggatg cttcctccat tcttcggcac ggggcgcgcg
     1441 gaatgccgcc tcgttgtggc gcgccgccgc gccgctcacc cagtgcagca gggggccgga
     1501 cgggtgatcc cgctgggtgg tggcgcaggc gctcacgcac tgggcgcact gggtgcaggt
     1561 gaacatggcg cgcttgatcg cccgcggctt gaggcgcatc gggcagacgt gctcgcaggc
     1621 ggcgtcgcac ccgacgcact gggccgcgcg ggcgcgctcg aagcccacca ccatggcgtc
     1681 ccggttaccc atccaggcca cgctctggaa catccccacc gcgcaggcga aacggcagaa
     1741 taaatgccgc gcgaagagga actcggcgct cagcacaagc gtcgcggcgc cgatgaagag
     1801 cgcctcgtta cgggtcggcg tacccgccag caggtggcca tacacctgga agggcggcag
     1861 cagataggtg agcagcacca ccgcccagac gaaggcgaag gccaccgcca ggggcacggt
     1921 ggcgccccac cagcgcggat ccaccggcag gggcgagccg tccggcgccc gggccggcag
     1981 ggcgtggggc tcccacacgc tgggcttgcc gctggcgcgg cgcatcagcc cgttgatcgt
     2041 ctccaccacc gagaagtgcg gacacagcca gccgcagtac agccggcccc agcgccaggc
     2101 cacgccgatc agcaccgcgc cggccgccag aatcggcagc aggacgcgca ccagcacccg
     2161 caggccggcc tccagggcac cgatgcgccc ggccgagaag tcttccagcc cggcatgcca
     2221 gggcatcccc aggaaccagg cgtggccggc caccaggtca tagcgcagca ggtcgaacac
     2281 cggcgccagc acgaacagca cgaagaaggc gccctgggcg aggcggcgct gggcctgacg
     2341 cccgccgtcc ggttgcgtcg cgggcacggc gtggatcggg atcacgcggg ccgggttcat
     2401 gcgacctccc gcgggcccac caccggaaag cggaaggacc gccccgccag cgcccgggac
     2461 aggcccaccg cgccgcacag gatcagggac gaatggacca gcgtgaaata gaggatgagc
     2521 accacccagg tagacgcggc gcggtagccg cccaaaagca ggatcgcccc gttggcgacg
     2581 atcagcaggg ccacggccca caggctcgcc gccatcgtct gacgcaggtg gctgcgcgcc
     2641 aggggcggcg cgtcccgatg acgcaggctc cacagcacgg ccaggacgat gaagccgagg
     2701 ccggggatca gcatcagatt ggccaggtag agggcctcgg cggccaccgc cagctcgcgt
     2761 ccggccacgt ccgcatcggc gggatcgacg gggagcatgt gcggcgcccg cgggcagtgc
     2821 gtgccgcgtt cagcgcccga aggtggcctg caccacctcg agcagcgcgt cggccacgtc
     2881 ggcatcgtcg gtgaggcttt ccaccagtgc cgagcggcag gcctccaccg gatccaggcc
     2941 caccttcacc agcttggcgg cgtaaaccag caggcgggtg gacaccacct cctccagatc
     3001 cacgtccttc agcgcccgca ggcggccggc gatggacacc aggcgcttgg ccagcatcgg
     3061 gtcgcagccg gtctcgccga tgacgatggc ctcttcccgc gccgccgcgg ggaattcgaa
     3121 acgcaggctg acgaagcgct ggcgggtgga cggcttgagg cccttgagaa agttctggta
     3181 gcccgggttg taggacatca ccagcatgaa ggcatccggc gcgtggaggg tctcgccggt
     3241 gcgctcgatg ggcagcacgc ggcggtcatc ggccagcggg tggatcacca cggtggtgtc
     3301 acggcgggct tccaccactt catccaggta gcagatgccg ccctggcgca cggcacgggt
     3361 caggggaccg tcacaccaca cggtctgacc gtcgcccagc aggtggcggc ccaccaggtc
     3421 ggcggcggtg aggtcgtcgt gacaggccac ggtgaacagg ggcaggccca ggcgcgccgc
     3481 catgtgcgcg acgaagcggg tcttgcccac gccggtgggg cccttgatga ggaggggcag
     3541 acggttcttc caggcctgtt cgaagacctg gatctcgttg ccggccgctt cgtagaaagg
     3601 aagattggca gtcatgggtg cgttcatcgg agtgcgatcc agtaggcgag ggcgatcagc
     3661 cccgcgacga cgagcagcca gcctaacagg agtgcgcgcc agaagaactt gaccccgcgc
     3721 aaacccatga aatcgagcac caccaggcgc cccttgacga agctgatggc cagcagcgcc
     3781 gccatggccg cgggaccggc agcgccgtgc tcgcccagtt cccaggtgag cgcggtggcc
     3841 accgcgagga cgacccacgc gaagttgagc tggcggacgt tcatcgcatc acgtacacca
     3901 gcggaaacag cacgatccac accagatcca ccatgtgcca gtaggccgcg ccgctctcca
     3961 ggccgttcat gcgccccgcg ccgtaggccc cgcgacgggt ctgcacccac agcacggcga
     4021 ggatcaccag ccccaggatc acgtgcatga agtgaaagaa ggtgagggac aggtagaaca
     4081 tgtagaaggt gttggtggac aggttgatgc ccgccccgaa cttggccgcg tattccacgc
     4141 ccttcacgcc caggaagccg gcgccgcaga ccagaccgcc cgccagccag cgcgcggccg
     4201 tggcggaacg atcgcgaccg gccgcctcca cggcgcgcgc cacgcagaag ctgccggtga
     4261 tcaggagcag ggtgttcacc gcgccggcgc gccggtccag ggtggcctgc atggcatcga
     4321 agagatcggg atgacgggcg cgggcaaagg cgtaggcggc gaagaacacg ccgaaggcca
     4381 gcagctcggc caggatgaag aaccagatcg ccaggtcgcc gggcggcgcc gcggcaggcg
     4441 cggggcgggc agatgtgctc atgcccggca gtgtcgccgg agcgtgccac cggagccttg
     4501 atctggcata agacgcgcac tctcccggcg tcgtaaattc ctgtcacacg ccccccgctg
     4561 atctatagct ttagtcgtag atgtcgttat ggcagggaga ttcttggacc cctcgcgcgg
     4621 aggcgcgccg atgaaaattc ttcttgtgga cgattcccgg gcggtggccg ccgtgatggg
     4681 cgcgcgcctg agcggtgccg gttacgaggt gatcctcgcc gagaacggca aggtggcggt
     4741 ggagcagttc cgcgccagcg cacccgatct ggtgctgatg gacatcgaga tgccggtcat
     4801 gaacggcttc caggcggccc aggcgatccg cgaactggaa gccaccggcg aatgggcgtg
     4861 gaccccgatc attttcctga ccgcctccga cacccccgaa aacctggtca ctgccattga
     4921 ggccggaggc gacgatttca tcgccaagat ccttccggaa gccgtgctgg aagccaagat
     4981 gaaggccatg gcgcgcatcg cccgcctgcg cgatcagctc tccgccgcca atcgcaagct
     5041 caccgagcaa gccagccagg acggtctcac cggcctgtgc aaccgtcgct acatggacct
     5101 gcgcctggac agccagtggg ccggctgctg cgatgcgcgt gagcccttcg ggctgctcat
     5161 gctggacgtg gaccacttca agaaatacaa cgaccactac ggccaccagg ccggtgacga
     5221 ctgcctcaag gccgttgcgc gggccatctc cgagaccgtc gaagccgcca acgtgcgggg
     5281 cgacgtgcat ggcgccctgg ccgcccgcta cggcggagag gaattcgcgg tgctgatgcc
     5341 ccgctgcacg cgcgacacgt tccgggccct cgccgacgcg atctgtacgg ccatccgcac
     5401 actcgcgatt ccccatgaac gcaacgcgga atggggcatc gtcacggcat cggtcggcgg
     5461 cgcctacgcc gcgcaggcgg aaggccgcgt cgccctgcgc ttccgggaag cagacgccaa
     5521 cctctaccgg gccaaggatc tgggccgcaa ccgggctgta ctggactgac gtccctgccg
     5581 gcccgtcaat agagccacgg aatcagccgc cgggtgcgcc gacgatagtc ggcgtaggcc
     5641 ggaaagcgat ccgccagcca gcgctcctcc cggcgtgact tcacatccag cagcacgaac
     5701 agcagggccg cccagccgag ggtcagccag ccctggtgga agagggccca accgaaggcc
     5761 gagaggatca gccccagata gatggggtgc cggacgcgcg cgtacaggcc gctcaccacc
     5821 agcgtcgcgc cgtccttggg atggggcagg ggggtgaggt tcgaacccag gcgcagggcc
     5881 cccagggcgg cgatgagacc acctgccgcc atcagcgcaa ttccgccgat cagcgccggc
     5941 agcgccgcct ccgccggcca ggggggcaga cccgccacgg tgcgcggtcc gaacacgagc
     6001 atgaccagca gcgccacctg gatcatcacg taggcctcgc cgcgcaggcc ctcccagcgt
     6061 actgtcggac cgttcaccgc atgctccctt ctgtcgccat acccctctcc acgtccatgc
     6121 gccccttgaa agccagcgca cgccgctcca cacagatcca cgacagaccc gccagcgcca
     6181 ccgtcagcgg tgccgagact gccagcagcc ccaccggcgt gatccctggc aacagcgcca
     6241 cgacggtctg ctgcacggga aaggcgtaca ggtagacccc gtaggagcag tctccgacgc
     6301 ggttgaagcg tcggatcacg ccgcccggca catgcgccag cgccaccacc agatagccca
     6361 gcagcagcac gtaggccacg aagaacacgt cccgctgcag actcgccagc gccagcaccc
     6421 cgcctccggc caacaggccg gaccacccca ggggtacccg tgccgccgcc atctgcaggc
     6481 acgcaccgca gaagaacatc cacgcgaagc gggcaaagta gcccgcatcc gtatgaaaga
     6541 aatggcggac cagcatcgtc ccgcccgcca cccccgcaat cagcgccacg gcgcccgtca
     6601 gcacgccctg ccgacgccga ccgagacgct gcacgacgat gcccagtaag gccaggatcg
     6661 cgtacatgcg cagctcgtaa ggcagggtcc acagggatcc attcaccgca gccggatagg
     6721 gattggcctc gaacaccccg ggcagatgga tcgcgatgcc gccccacagg ctggcacatt
     6781 tgatcaggta gtcgaccacc ccggggtcgg cccagtaagc cgccgccggg cgcgtcgtga
     6841 gcagtggccc gagcacggcc acggtcagca gcaccacccc ccacagcgcc gggtagatgc
     6901 gcagcacccg tgcccacaca aaggccctca cccccggacg gtcacgcagg ctggccgtca
     6961 ccaggaaacc gctggtcacg aagaacacat ccacggcgac gctgccgatg tcgatgccca
     7021 gccagtccgc caacggcggc gccccgcctc ctgcgagggg gtagcaatgc ccatagagca
     7081 ccgccagggc cgccaggaag cggatcaggt ggaaattgtt gtcccgcccg cgggccgcct
     7141 cggccagcgt cacgcgagct ccggggggcc ggacgacgcg gtcgtcagtc cgctgcaatc
     7201 atttcgcatt gcaccattgt agccgctgca ggcaaggctg catgcaatgc gggaggctgc
     7261 gggaaagaaa ccttacaatg cagtgacaga cacgtcgcgc ccgggacaag ggcgcacgac
     7321 cacaggagga gacaccatgg gcgccatctt cgccaagacc cgcaaagggc agcaggaaat
     7381 cgagacccgt tccggcgggc tccacccgcg ggtgcgtcgc accctcatcg tcgtcgacgg
     7441 caagcgcagc gtcgacgagc tgcgcgaagc gctgcggagc gacgacctgc agcacaccct
     7501 cggcctgctc gaagaggagg gctacatcga gctgatctcc ggcgcacggc tcgcggaggc
     7561 tgcaccggcg tccatcagcg ccttccggct ccatccgccg ggggaagatc cgctgcgcct
     7621 gcagcagagc cgcaatttca tgctcaacac cctgcgcacc ttcgtcggcg cggtgggcac
     7681 cagctccctg caggaccgcc tcgaggcggc gcgcacccat gccgacctgc gcgccctctt
     7741 cgacgagtgg taccacgccc tggtcacctc ccgggatggc cgccgcgagg ccgaacacct
     7801 gcgcagcaag ctcctcgaga tcctgtaggc cagccgcgct cagctcccct tggggggcag
     7861 gccggccaca taaaggcggg ccgcctccac ccaggccgcc agatccgcgt cctcggcgaa
     7921 gccgtccggt gccacgtaga catagccacg caagggtcgt ccggtgaagt ccatcggccg
     7981 ggcatggggg cgggcgagcg cggcggcata ggcatccggt ccgacccgga ccatcagcgt
     8041 ctcgccgacg atgccgacac acatgtgacc ccgccacatc agcgcgagcc cgccgaacat
     8101 gcgccgctcg cccacgtccg tcattccggc gaacgcctcc cgcagacgct ccgccagccc
     8161 ttcgtcgaat gccatcccgc cctccgcccg tcctgacgtc tgcttacagt taagggggca
     8221 ctttcggcgc ccgtgaggct cgaaggcctc gccatcccct tctgcgaggc ctgcgtcgat
     8281 gttcaagaag ctgtccgccc tctacaccct gatccgcgaa gacctgcgca tcctgggctt
     8341 cgccctgcgt catccggcgc gtccggtctg gctgctgcca gccctcgtgg cgctggggct
     8401 gtacgtcctg tcgccgatcg acctgctgcc ggacctggtg ccggtactcg gctgggtgga
     8461 tgacctgatc ctggtccccc ttgtcatcgc ctggctggtg agccggctgc cggtcgacct
     8521 gcgccgcgcc tggcaggatg cccggcggac cgtggatgcg cccgccgtgc gccgcgcttg
     8581 aagcctcagg cgcgcaccac cgccgccgaa aagagctgga gcaccaccac cccggcgacg
     8641 atcaggccga tgccgatcag cgccgggcgg tccagcttct gaccgaagaa ataacgcccg
     8701 accagagtca tcagcgccac cccggagccg gaccacaccg cgtacaccac ccccaccggc
     8761 agcacgtcca gggtgaggga caggagatac agggccagcg aatagcagct caccacgatg
     8821 ccccagggcc acaggcgcct gaaaccgtcg gccagcttga gggccgaggt agcgatgacc
     8881 tccgcgacga tggcgacgaa gagcagcagc cagtccatgt cagcctcccg ccgcgttcat
     8941 tcggtcaccg tctcggagaa ggcgttgatc accaccaccc ccgccacgat cagcccgatg
     9001 cccagcagcc ccgccggatc cagcgtctga tccagaaaga cccacgccac cacggtgatc
     9061 agcgccacac ccacgccgga ccacaccgca tacacgatgc ccacggggat tttcttgagg
     9121 ctcagggaca gcaggatgaa ggccgtctcg tagcacacca cgacgaccac cgacggccac
     9181 aggcgggtga agccttcggt ggccttgaga gtcgacgtgg cgatcacctc cgcaacgatg
     9241 gccacgaaga gcagcaccca ctgcagggtg ccggcccggg gcgcaggggc cggcgtggcg
     9301 gtcggatcgg gcgcagccat cagcgcgtcg ccgcctcgat gtccctgcgg cgctgctccg
     9361 cctgcaggcg cgcagcttcg agctggcttt ccacctgggc cttgacggcc tccgcctgct
     9421 gcgcctcctg ggccttgagc ttggccgcgg tggccgcgct gctgccggca tcaccaaagc
     9481 cgcaggccgc caagccggtg gccagcaggc cggccagaac gaaggccctc atgcggcctc
     9541 ccagcgcggg taatccagat agcccttctc accgccctgg tagaaggtgt ccatatccca
     9601 ctccgccagc ggcgcgccgg cctgcaggcg tgccggcagg tccgggttgg agatgaacag
     9661 gcggccgaac accgcgccat cggccgtgcc ctgcgccacc agctcggccc cacgctgcgg
     9721 cgacagtccg ccattgacca ggatgggtgt gcgggtgaag gcgcggaagt gggccgccac
     9781 ggcatcgagg ggtcgggcca cgccgtcgag ctggcccggc tggaaaggct cggtgagatg
     9841 caggtaggcg aggcccagac catccgccgt gcgcaccaga tcgtcgaaga agggcacggt
     9901 gtcttcgtcc acgacgatgc cgtggagcgt gttcatcatc gggttgaggc ggatgcccac
     9961 ccggccgagg ggcagctcgc ggctcaccgc gtcgagcacc tcaaagagca ggcgagcgcg
    10021 gttcgcgtgg ctgccgccgt actcgtcgtc gcggacgttg ctgcagcggg caaagaactg
    10081 gtggaacagg tagccgttgg aggaatggat ctccacgcca tcgaagcccg cttcacgggc
    10141 atgggcggcg gcgcgggcga agtcggcgac gatgccagcg atctcctcgc gggtgagggc
    10201 gcggggggtg accgtgggcg tcaggcctgc actaccgaac atcttccact ggggatcgat
    10261 ggccgagggc gccaccggcg ccgcgccgcc gtggaattcc ggcagggaca ggcggccgca
    10321 gtgccacagc tggcagacga tgcgcccgcc ggcggcgtgc accgcgtcgg tgacgaggcg
    10381 ccagcccgcc acctgcgcgg ccgaccagag gcccggcgta aaggggtagc cgcgcccctg
    10441 gggcgacacg atggtgcctt ccgaaacgat caggccggcg ccggcccttt gggcgtagta
    10501 ctgcgcctga gtggcgtccg gcgccgcctc cccattggtc acacgacagc gcgtgagcgg
    10561 cgccatgacg atccggttgg ccagatcgag atcgcccagc cggaccggct ggaagagggc
    10621 ggaaacagca gcagggggac tcatgacggg gcctttcgac gatgacggca gcacgccgcg
    10681 atggtagcaa atccggatgg cgggtggtcc cggaccgagt gggagatgtc tgtccgtaag
    10741 ctttcaaggg gctggcaatt ctttgcacag atcaaggaaa ccacctggcc gggtgcctag
    10801 actgactctg tttcgatcca ccttttcctg ttcatggaca tcacccgccg gcgcttcctg
    10861 ggcggacgca tcggacggaa cccgggcccc gcgcccttcc gccccccctg gagcctcccc
    10921 gaggccgcct tcctcaatca atgtacacgc tgtgacgact gcgtgaaggc ctgcccgacc
    10981 ggcctgctgc gccgtgcgga cgatggcttc ccggtggcgg acttcagcgc tgccgaatgc
    11041 accttctgcg gcgactgcgt gccggtctgc ccgcccggcg ccttggtgcg cgtcggcgac
    11101 gccgccccct ggtccttcgg tgtgcggatc ggcgaaggct gcctggccct gcagcaggtg
    11161 gaatgccgca tctgcggcga ggtctgtgat ccgcgggcga tccgcttccg tccgcgtcct
    11221 ggcggcatcg ccccacccga ggtggacaac acctcatgca ccggttgcgg tgcctgtatt
    11281 gcgccctgtc cggtggtggc cgtaacgcgc atccccctga ccaccgcccc ggaggcccca
    11341 tgaacatcgt cagcctggta ctgcgcatcc atcccgacac ccgcgccgag gcggaagccg
    11401 cgctcaccgc ctttcccggc gtggaatgtc acggcatgtc cgacgacggc aagctcgtcg
    11461 tcacggtgga ggacgcggaa ggtgcttcca tgtccgacac cctgatcgcc ctgcaccggg
    11521 tgccccaggt gctcgccgcg acgctggcct acgaatacac cgaccgcacc gccgccactc
    11581 cgacccacgc cccgaattcg aattccccct gcgaggaggc tcaatcatga ccatcaatcg
    11641 acgcgacttc atcaaggccc aggccatcgc caccgccgcg gccaccgccg gcatttccct
    11701 gcccgtcgtg gcggccccca aggaggcgcc cgccgccgcg ggcggcgaca cccgggtgcg
    11761 ctgggacaag gccgcgtgcc gcttctgcgg taccggctgc tcggtgctgg tgggcgtgca
    11821 ggatggccgc gtcgtggcca cccaggggga tcccgacgcg ccggtgaacc ggggcctcaa
    11881 ctgcatcaag ggctatttcc tgtccaagat catgtacggc gaagaccgcc tgaccaagcc
    11941 cctgctgcgc atgaaggacg gcaagtacga caagaacggc gagttcacgc cgatcagctg
    12001 gaaccaggcc ttcgacatca tggccgagaa gtggaaggcc gccctgaagg ccgaaggccc
    12061 cggctcgatc ggcatgttcg gctcgggtca atggaccatc tgggaaggct acgccgcctc
    12121 caagctgatg aaggccggct tccgctccaa caacctggac ccgaacgccc gccactgcat
    12181 ggcctcggcg gtcgccggct tcatgcgcac cttcggcatc gacgagccga tgggctgcta
    12241 cgacgacatc gagcaggccg acgccttcgt gctgtggggc tccaacatgg ccgagatgca
    12301 ccccatcctg tggacccgca tcaccgaccg ccgcctcacc aagaccgacg tgcaggtgca
    12361 cgtgctgtcc accttcgagc accgcagctt cgagctggcc gacaacggca tgatcttcgt
    12421 gccccagacc gatctggcga tcctcaatta catctgcaat tacatcatcc agaacaacaa
    12481 ggttaaccag gagttcgtca agaagaacgt caacttcaag atgggcgaga ccgacatcgg
    12541 cttcggcctg cgccccgccc acgcgctgga gaaggacgcc aagttcaacg gctaccccgg
    12601 cgccgacggc aagcccaaga acaacccgaa cgacgccaag cccatcacct tcgacgagtt
    12661 caagaagttc gtctccacct acactctgga ctacaccacc aagctcaccg gcgtgccggc
    12721 cgagcgcctc aagaagctcg ccgagctcta cgccgacccg aagaagaagg tggtcagctt
    12781 ctggaccatg ggcttcaacc agcacacccg cggcacctgg gtgaacaaca tgatctacaa
    12841 cgtccacctg ctggtgggca agatctccac cccgggctcc ggccccttct ccctcaccgg
    12901 tc
//
LOCUS       DAOQPA010000101        12834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2514_length_12834_cov_20.322639, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000101 DAOQPA010000000
VERSION     DAOQPA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_12834_cov_20.322639"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1399
                     /locus_tag="PKX01_13105"
     CDS             116..1399
                     /locus_tag="PKX01_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ77114.1"
                     /translation="MKIETLAVHAGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVAGNIYTRIMNPTQAVLEARVAAMEGGIAGLALASGMAAITYAIQTIAEAGDNIVS
                     ASTLYGGTYNLFAHTLPQIGIQVRFADYRDPDSFAPLIDERTKAIYCESVGNPLGNIT
                     DFEALAAIAHAHGVPLIVDNTVPSPYLCRPIEHGADIVVHSLTKYLGGHGNSIGGLIV
                     DSGKFPWAEHKARFRRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAISPFN
                     VFLILQGIETLTLRMDRICKNTRKVAEFLKGHAKVAWVNYAGLPDHPDHALVQKYMGG
                     RASGILNFGVKSADGDGKAAGARFQDALQLVTRLVNIGDSKSLACHPASTTHRQLSPD
                     EMARAGVSEDMVRLSIGIEHIDDILADLDQALAAV"
     gene            1563..2126
                     /locus_tag="PKX01_13110"
     CDS             1563..2126
                     /locus_tag="PKX01_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HMZ77115.1"
                     /translation="MNAHLLTHSRRVALRGLAAGVGLALMLTPPAQADDLQLLIKHGT
                     VGGRFERNGIGLRFGTLWTSMWGPWTAALKPELEFSRLRYSGTADAPHSLDEAGILGV
                     LRVTHPGAGLQAYVEAGLGGALFSHDRLGDKTFSTHFQFSEHVGAGVTLGGRASLGWQ
                     FSHYSNADIRLPNDGLDVHQIVLAIRF"
     gene            complement(2191..3897)
                     /gene="leuA"
                     /locus_tag="PKX01_13115"
     CDS             complement(2191..3897)
                     /gene="leuA"
                     /locus_tag="PKX01_13115"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMZ77116.1"
                     /translation="MLKNPNTKYSPFPPVALADRQWPSRTITHPPVWMSTDLRDGNQA
                     LFEPMNAARKLQMFRQLVAIGFKEIEVAFPSASQTDFDFVRQLIEGGEIPDDVTIEVL
                     SQAREHLIRRTMESLRGARRAIVHIYNATSRNFRETVFGMSRDEVKAMAVDSVRLVKT
                     LAAEQPTTEWVLEYSPETFTATELDFAREVCDAVVAAWDPRPGEKVILNLPSTVEMAT
                     PNVYADQIEWMHRHLARREQIILSVHPHNDRGTAVAAAELAVLAGAERVEGCLFGNGE
                     RTGNVDLVTLALNLYTHGIDPGLDFSDINTVVRVAEACTQLPVHPRHPYAGDLVFTAF
                     SGSHQDAIKKGLQVQQPDTLWQVPYLPVDPADLGRTYDSVIRVNSQSGKGGVAHLLES
                     AYGLVMPRRMQVEFSGAVQRLTDETGQEVDAHQLWQLFCREYLDADAPVRYGDHHLYE
                     ADGRQGIRIEAHIDDQAQVLKGEGNGPVEAAVAALRVPVRVQSFEERSMGEGADAVAM
                     AIVEVAMPGVAGTCFGAAMHRNTVTASVRAVVAAVNRILARQGGGANPGAVEAVQAGC
                     AA"
     gene            complement(4244..5704)
                     /locus_tag="PKX01_13120"
     CDS             complement(4244..5704)
                     /locus_tag="PKX01_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77117.1"
                     /translation="MVLGEKDREPHPLLDEQVLRRVLTDISTSAAPAALGELIRLLAS
                     MPDVRDMDVRNTARQVFALDEAAQAQVRQAAGLYLSNAYSARSVRYKALGQDFHAFLG
                     QVYDLLMSGLIGDFTIPRDDPLRAEILLRTVRTAAGEFKWAAFDYQSLSPEAWRRAMS
                     AYRLALSAGVADLPVRLREGRESRTTVSREFARLVAMQCAGFEQLSPERIEAADKLMR
                     FVQHALRLTGEPLEGSQFSVDLDGFERPRRLLNLPEAAGTLRFFRPGDAVPVLGDLAG
                     SVLLPGGHAAFAGTNPVEIGAAIAHLNRQWGARAPMRQYRRHPMRGEVSLSTGLGLIR
                     SLISGEAQFRPAPAWHLVDASRDGFCLASRLFDGEVCRVGTLVGAYLGEWGTWTLAVV
                     RRVQHSERQGTSIGLQILTSGPRPAALDDGSRSWLGVLCDPVVRGRALRIVCEPGFTG
                     LGGRLFVKQGPRTVKADVEHLVARGPGFEVWGCKVR"
     gene            5874..6668
                     /locus_tag="PKX01_13125"
     CDS             5874..6668
                     /locus_tag="PKX01_13125"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMZ77118.1"
                     /translation="MRQYLDLMRHVLEHGHRKDDRTGTGTLSVFGWQMRFNLADGFPL
                     LTTKKLHTRSIIHELLWFLQGDTNIRYLKDHGVSIWDEWADDQGDLGPVYGKQWRRWE
                     TADGRSIDQIAQLIAGLKGNPDSRRHIVSAWNPGDVERMALPPCHALFQFYVAGGRLS
                     CQLYQRSADIFLGVPFNIASYALFTMMVAQVCGYAPGDFVWTGGDCHLYANHLDQAHL
                     QLSREPRPLPTLRINPDVKDLFAFRFEDFSLEGYDPHPHISAPVAV"
     gene            6665..7165
                     /locus_tag="PKX01_13130"
     CDS             6665..7165
                     /locus_tag="PKX01_13130"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMZ77119.1"
                     /translation="MTRLALIAAVARNGIIGVANRLPWHLPEDMKFFRETTRGQPVIM
                     GRKTWESLPDTFRPLPGRHNIVVSRNPDFRAAGATVVTSLPDALTTAGDADTAYLIGG
                     AELYRQALPLADRLVLTEIDRDFAGDAHFPEFDRAAWQEVARDARVSESGLPFAFVRY
                     ERSPAD"
     gene            complement(7261..9504)
                     /locus_tag="PKX01_13135"
     CDS             complement(7261..9504)
                     /locus_tag="PKX01_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HMZ77120.1"
                     /translation="MRFNFPVIVIDEDFRSENTSGLGIRALAEAIEKEGLQVVGVTSY
                     GDLASFAQQQSRASTFILSIDDEEFSSPEAAAKAIADLRAFVSEIRLRNADIPIFLHG
                     ETRTSRHIPNDVLRELHGFIHMYEDTPEFIARNVVREARAYMDALPPPFFRALTHYAS
                     DGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAA
                     SERNAARIFNCDHLYFVTNGTSTSNKIVWHSTVAPGDVVVVDRNCHKSVLHAIMMTGA
                     IPVFLMPTRNNYGIIGPIPKSEFAWENIQKRIAANPFITDKNAKPRVLTITQSTYDGV
                     LYNVEAIKEELDGLIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRCKESMVFSTQST
                     HKLLAGLSQASQILVQDAEHQRLDRDVFNEAYLMHTSTSPQYAIIASCDVAAAMMEEP
                     GGTALVEESIAEALDFRRAMRKVDEEWGADWWFKVWGPDDLSEEGIEEREAWMLKPGE
                     RWHGFGRLAEGFNMLDPIKATIITPGLDVDGEFAERGIPAAIVTKYLAEHGVIVEKCG
                     LYSFFIMFTIGITKGRWNSMVTELQQFKDDYDKNQPLWKVLPEFVQKNPRYERVGLRD
                     LCREIHSVYRANDVARLTTEMYLSDMIPAMKPADAFAKMAHREIERVPIDALEGRVTS
                     VLLTPYPPGIPLLIPGERFNRTVVRYLQFAREFNERFPGFETDVHGLVKEVKDGRIHY
                     FVDCVRD"
     gene            complement(9616..10185)
                     /gene="dcd"
                     /locus_tag="PKX01_13140"
     CDS             complement(9616..10185)
                     /gene="dcd"
                     /locus_tag="PKX01_13140"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMZ77121.1"
                     /translation="MSIKSDKWIRRMAEGDAKMIEPFAPELVRQNAGGKIVSYGTSSY
                     GYDVRVANEFKIFTNINSTIVDPKAFDERNFVDFTGDVCIIPPNSFALARTVEYFRIP
                     RSVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLF
                     FESDEICDTSYRDRGGKYLGQTGVTLPKI"
     gene            complement(10295..10480)
                     /locus_tag="PKX01_13145"
     CDS             complement(10295..10480)
                     /locus_tag="PKX01_13145"
                     /inference="COORDINATES: protein motif:HMM:NF038104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038104 family lipoprotein"
                     /protein_id="HMZ77122.1"
                     /translation="MRRLRAWGPAVVLAASLSGCVVGTVVGTAVDVGVEVVKVPFKVG
                     KGVYDAVKDDDVKDAGR"
     gene            complement(10480..11571)
                     /gene="apbC"
                     /locus_tag="PKX01_13150"
     CDS             complement(10480..11571)
                     /gene="apbC"
                     /locus_tag="PKX01_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HMZ77123.1"
                     /translation="MSLTEQSILDSLRQYADPNTGKDYVTSRAVKNLRLHGADVSFDI
                     ELGYPAASQIDTIRQAVIACAKSVPGVGNVSANVFTRIVAHAVQQGVKLLPGVKNIIA
                     VASGKGGVGKSTTSANLALALAAEGATVGILDADIYGPSQPQMLGIGDSQPESRDGKT
                     MEPLEAHGLQVMSIGFLVDVETPMVWRGPMATQALNQLLKETNWRDLDYLVIDMPPGT
                     GDIQLTLSQSVPVTGAVIVTTPQDIALLDARKGLKMFEKVGVPILGIVENMSIHICSQ
                     CGHEEHIFGSGGGATMGEDYGVPFLGALPLDIQIRREADGGTPTVAADPDGRVAQVYK
                     EIARKVAIAVAERQKDMSHKFPSIVVQNT"
     gene            11748..>12834
                     /gene="metG"
                     /locus_tag="PKX01_13155"
     CDS             11748..>12834
                     /gene="metG"
                     /locus_tag="PKX01_13155"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMZ77124.1"
                     /translation="MTTRKILVTNALPYANGDIHLGHLVGYIQADIWVRYQRMRGHTV
                     HYVCADDTHGTPVMLRAEKEGLTPEQLIARVHGEHLRDFTDFGVAFDHYHSTHSSENR
                     WYAEDIYTRLRDSARLIETRAIEQFYDPVKEMFLPDRFIKGECPKCGAQDQYGDNCEV
                     CGAAYAPTDLKHPRSAVSGAEPVLKTSEHYFFRLSDPRAVEFLREWTQGTTPEGERRL
                     QAEAANKMKEWLGDSGENALSDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYFASF
                     RHLAILRGDIDVADYTEAGPAAATGTELIHFIGKDILYFHALFWPAMLHFAGYRTPTQ
                     LCVNGFLTVDGAKMSKSRGTFITARSYT"
BASE COUNT         2227 a         4441 c         4066 g         2100 t
ORIGIN      
        1 ggccctggac gcccctgcct gacccgcctg cgcgggctgg gtatactccc cacacagctc
       61 cccacaccga attccgatca cacacaatca caccacacac aggagaccgc tacccatgaa
      121 gatcgaaacc ctcgccgtcc acgccggcta cagcccggac cccaccacca aggccgtcgc
      181 cgtgccgatc taccagacca cctcctacgc gttcgacgac acccagcacg gcgccgacct
      241 gttcgacctg aaggtggcgg gcaacatcta cacccgcatc atgaacccga cccaggccgt
      301 gctggaggcc cgtgtggcgg ccatggaagg cggcatcgcc gggctggccc tggcctcggg
      361 catggcggcc atcacctacg ccatccagac catcgccgag gccggcgaca acatcgtctc
      421 cgcctccacc ctgtacggcg gcacctacaa cctgttcgcc cacaccctgc cccagatcgg
      481 catccaggtg cgctttgccg actaccgcga cccggactcc ttcgcgcccc tcatcgacga
      541 acgcaccaag gccatctact gcgaatccgt tggcaacccc ctgggcaaca tcaccgactt
      601 cgaagccctc gccgccatcg cccacgccca tggcgtcccg ctgatcgtgg acaacacggt
      661 gccctcgcct tacctgtgcc gccccatcga gcacggcgcc gacatcgtcg tgcattccct
      721 caccaaatac ctgggcggtc acggcaactc catcggcggc ctcatcgtgg acagcggcaa
      781 gttcccgtgg gccgagcaca aggcccgctt ccgccgcctc aacgagccgg acgtctccta
      841 ccacggcgtg gtctacaccg aagccctcgg cccggccgcc tacatcggtc gcgcgcgggt
      901 cgtgcccctg cgcaacatgg gcgccgccat cagcccgttc aacgtcttcc tgatcctgca
      961 gggcatcgaa accctcacgc tgcgcatgga ccgcatctgc aagaacaccc gcaaggtagc
     1021 tgaattcctg aaggggcatg ccaaggttgc gtgggtgaat tacgccggcc tgcccgatca
     1081 cccggaccac gccctcgtcc agaaatacat gggcggccgc gcctccggca tcctcaactt
     1141 cggcgtcaag agcgccgacg gtgacggcaa ggccgcaggc gcccgcttcc aggacgccct
     1201 ccagttggtc acgcgcctgg tgaacatcgg cgacagcaag agcctcgcct gccacccggc
     1261 ctccaccacc caccgccagc tctcgccgga cgagatggcc cgcgccgggg tgtccgagga
     1321 catggtgcgc ctgtctatcg gcatcgagca catcgacgac atcctcgccg atctggacca
     1381 ggccctcgct gcggtgtgat ccctgaccgc cccgcgcgag cgacccgcat ggcggcgcct
     1441 gccagtgtcg ccatgtgtgc ccgccggatc ctgaacccca tgccggcggt gcggtcccac
     1501 ggggacagga aggccagtcc cttgcgtggc atcccaagcg ccacccgatc cgcgagacgg
     1561 tcatgaacgc acacctcctt acgcactccc ggcgcgtcgc cctacgtggg ctggcggccg
     1621 gcgtcggcct tgccctgatg ctcacacccc cggcccaggc cgacgatctg caactgctca
     1681 tcaagcacgg cacagtcggc ggacgcttcg agcgcaacgg catcggtctg cgcttcggca
     1741 ccttatggac ttccatgtgg ggcccatgga cggcagcgtt gaagccggaa ctggaattca
     1801 gccgtctccg ctacagcggg acagccgacg cgccccacag cctggacgag gccggcatcc
     1861 tcggtgttct ccgcgtcacc catcccggcg ccggtctgca agcttacgtg gaggcggggc
     1921 tcggcggcgc cctgttcagc cacgaccgcc tgggcgacaa gaccttctcg acccacttcc
     1981 agttttccga acacgtgggc gcgggcgtga ccctcggcgg gcgggcatcc ctgggctggc
     2041 agttttcgca ctattccaat gccgacatcc gactccccaa cgacgggctg gatgtgcacc
     2101 agatcgttct ggccatccgt ttctgaacgc cgccaccggg cacaaaaaac gggggccgaa
     2161 gcccccgcct gacgcggcga gcctgtcggc tcaggcagca cagcctgcct gaaccgcctc
     2221 aaccgcaccg ggattcgccc caccgccctg gcgggcaagg atgcgattga ccgcggcaac
     2281 aacggcccgc acggaggccg tcaccgtatt gcggtgcatc gccgccccaa agcaggtccc
     2341 ggcgaccccc ggcatggcca cctcgacgat ggccatcgcc accgcgtcag ccccttcgcc
     2401 catggagcgc tcctcgaagc tctgcacccg caccggcacg cgcaaggccg ccacggcggc
     2461 ttccaccggt ccgttgccct cgcccttcag cacctgtgcc tggtcgtcga tgtgcgcctc
     2521 gattcggatg ccctgtcgcc cgtccgcctc gtagaggtgg tgatcgccgt agcggacggg
     2581 cgcgtcggca tcgaggtatt cgcggcagaa gagctgccac aactgatggg catccacttc
     2641 ctgcccggtc tcgtcggtga ggcgctgtac ggcgccgctg aactccacct gcatgcgccg
     2701 gggcatgacc agaccgtagg cggattcgag aaggtgcgcg acgccgccct tgccggactg
     2761 gctgttcacc cggatcaccg agtcgtacgt acgcccgagg tctgccggat ccaccggcaa
     2821 gtagggcacc tgccagagcg tgtccggctg ctggacctgc aagcccttct tgatggcgtc
     2881 ctggtgcgac cccgagaagg ccgtgaacac gagatcgccc gcgtagggat ggcgcggatg
     2941 gacgggcaac tgggtgcagg cttcggccac ccgcacgaca gtgttgatgt ccgaaaagtc
     3001 gagccccgga tcgatgccgt gggtgtaaag gttgagggcg agggtcacaa gatccacgtt
     3061 gcccgtgcgc tccccgttgc caaagagaca gccctcgaca cgctcggcac cggcgagcac
     3121 ggcgagctcc gccgccgcca ccgccgtgcc ccgatcgttg tgaggatgca cgctgaggat
     3181 gatctgctcg cgccgcgcga gatggcgatg catccactcg atctggtccg cgtagacgtt
     3241 gggcgtggcc atctccacag tggacggaag gttgaggatg accttctcac cgggacgcgg
     3301 atcccaggcg gccaccacgg catcgcagac ctcacgggcg aagtcgagtt cggtggcggt
     3361 gaaggtctcc gggctgtact cgagcaccca ttcggttgtg ggctgctcgg cggcgagcgt
     3421 cttgacgagg cggacactgt ccaccgccat ggccttcacc tcgtcccggc tcatgccgaa
     3481 gacagtctcc cggaaattcc gcgaggtggc gttgtagatg tgcacgatgg cccgacgggc
     3541 gccgcggagg gattccatgg tgcggcggat gagatgctcg cgggcctggc tgagcacctc
     3601 gatggtgaca tcgtccggga tctcgccgcc ctcgatgagc tggcggacga agtcgaagtc
     3661 ggtctgggac gcggagggaa aggcgacctc gatttccttg aagccgatcg ccacgagttg
     3721 ccggaacatc tgcaacttgc gcgccgcgtt catcggttcg aagagggcct ggttgccatc
     3781 gcggagatcg gtgctcatcc acaccggcgg atgggtgatc gtgcggctcg gccattggcg
     3841 gtcggcaagg gcgaccgggg gaaaggggga atacttggtg ttcgggttct tcaacatgat
     3901 cgtcgtatcc tgggctgtcg tcggaaatgg aattcaggag cgggcgcaag gctgccaggc
     3961 tgtcgcgggc tcgtggcctg gcaaccgggt cgcagtcgca gggacagcag gaagatgttc
     4021 agggtggaca tgacaagact ccggggaaaa cgcgtgcaga gacgcaacgt gcccacaagg
     4081 ggcaggattc gggacaacag ggaaggggaa agaaagtgtg cccgttaggg cagcagcaga
     4141 cccagcccgg aacgggccag aagggagaag agagcggtct gttgcgaggc gtacatgcgt
     4201 cgaaccttag cagcccgcct gtaccgtgtc aacgcagcgc gcgctatcgg accttgcagc
     4261 cccagacttc gaagcccggc ccacgggcca cgaggtgctc gacgtccgcc ttgacggtgc
     4321 gcggcccctg cttgacaaag agacgcccgc ccagcccggt gaaacccggc tcacagacga
     4381 tgcgcagggc gcgcccacgg accaccgggt cgcagagtac cccgagccag cttcggctgc
     4441 cgtcatccag cgccgcgggg cgcgggccgc tggtgaggat ctgcagcccg atggatgtgc
     4501 cctgccgctc actgtgctga acgcggcgca ccacggccag ggtccacgtc ccccattcgc
     4561 cgagataggc ccccaccagc gtgccgacac gacacacctc accgtcgaac aggcgcgacg
     4621 caaggcagaa gccgtcgcga ctcgcatcca ccagatgcca tgcgggcgcc ggccgaaact
     4681 gagcctcacc cgagatgagc gaccggatca gacccagccc ggtggacagg gacacctcgc
     4741 cacgcatggg atgacgccga tactggcgca tgggcgcacg cgcgccccat tgccggttga
     4801 ggtgggcgat ggccgcaccg atctcgactg gattggtacc ggcgaaagcc gcatgccccc
     4861 cgggcagcag caccgatccc gccagatcac ccagtaccgg caccgcgtca ccaggccgga
     4921 agaagcgcag cgtgccagcc gcttcgggca gattgaggag acgccgcggc cgctcgaagc
     4981 catccagatc cacggaaaat tgactgcctt ccagcggttc gccggtcagc cgcagggcat
     5041 gctgcacaaa gcgcatcagc ttgtcggcgg cctcgatgcg ttctggcgac agctgctcga
     5101 agccggcgca ttgcatggcc accaggcgcg cgaattcgcg gctgaccgtc gtccggctct
     5161 cgcgcccctc ccgcagccgc accggcaggt cggccacccc ggcggacagc gccaggcgat
     5221 aggcgctcat ggcccgccgc caggcttccg gcgacaggga ctgataatcg aaggcggccc
     5281 acttgaactc gcccgcagcg gttcgcaccg tgcgcagcag gatctcggcg cgcagcgggt
     5341 cgtcccgcgg aatcgtgaag tcgccgatca gcccggacat caacagatca tagacctgcc
     5401 cgagaaaggc gtgaaaatcc tgcccgaggg ccttgtagcg cacgctgcgg gcactgtagg
     5461 cgttggacag gtaaagcccc gcggcctgcc gcacctgggc ctgggcggcc tcgtccaggg
     5521 caaagacctg gcgcgcggta ttgcgcacgt ccatgtcgcg cacgtcgggc atggacgcca
     5581 gcaggcggat gagctccccc aaggctgccg gggcagcact cgtggagatg tcggtgagca
     5641 cgcggcgcaa gacctgctcg tcgagcaggg gatgcggttc ccggtctttc tccccaagca
     5701 ccattttctc acctcgcggg ccgccgccgc ccacgtaaag ttttgtcaat ttcagaagat
     5761 gttagcacac ggatagcacg taccgggtgc cgacatgttc gcgctgcggg ccgcctggcg
     5821 gcggtgcgaa cgccgtggca tcaggtacac tggcggcttt gcctgccctc accatgcgac
     5881 agtatcttga cctcatgcgc cacgtgctgg agcacggcca ccgcaaggat gaccggaccg
     5941 gcaccggcac tttgtccgtc ttcggctggc agatgcgctt caatctggcc gacggcttcc
     6001 cgctgctcac caccaagaag ctccacaccc gctccatcat ccacgagctg ctgtggttcc
     6061 tgcagggcga caccaacatc cgttacctca aagaccacgg cgtgtcgatc tgggacgaat
     6121 gggcggacga tcagggcgac ctgggcccgg tctacggcaa gcagtggcgg cgctgggaaa
     6181 ccgcggacgg gcgcagcatc gaccagatcg cccagctcat cgccggcctc aagggcaacc
     6241 cggactcccg ccgccacatc gtctccgcat ggaaccccgg tgacgtggag cgcatggccc
     6301 tgccaccctg ccacgcgttg ttccagttct acgtggccgg cggacgcctc tcctgccagt
     6361 tgtatcagcg cagcgccgac atcttcctcg gcgtgccctt caacatcgcc agctacgccc
     6421 tgttcacgat gatggtggcc caggtgtgcg gctacgcgcc gggcgatttc gtatggaccg
     6481 gcggcgactg ccacctgtac gccaatcacc tggaccaggc ccacctgcag ctgtcccgcg
     6541 agccgcggcc gctgccgacc ctgcgcatca acccggacgt gaaggatctg ttcgccttcc
     6601 gcttcgagga cttctccctc gaaggctacg acccgcaccc ccacatctcc gcgccggtgg
     6661 cggtatgacc cgcctcgccc tgatcgccgc ggtggcgcgc aacggcatca tcggcgtcgc
     6721 caaccgcctg ccctggcacc tgccggagga catgaagttc ttccgcgaga ccacccgcgg
     6781 ccagccggtg atcatgggcc gcaagacctg ggagtccctg cccgacacct tccgccccct
     6841 gcccggccga cacaacatcg tggtgagccg caacccggac ttccgtgcgg ccggcgccac
     6901 cgtggtgacc agcctgcccg atgcgctgac caccgcgggc gacgcggaca ccgcctacct
     6961 gatcggcggc gccgagctct accgccaggc cctgcccctg gccgaccggc tggtgctcac
     7021 agaaatcgac cgggatttcg ccggggatgc gcatttcccg gaattcgacc gcgcagcgtg
     7081 gcaggaagtc gcccgcgacg cacgcgtgtc agaatccggg ctgcccttcg ccttcgtccg
     7141 ttatgaacga agtccggcgg actgaccacc ggcaccttcc aacagcccca cccgcaacaa
     7201 aaatgccgcg atcgctcgcg gcattttgca tgcagcgccg tgggtggcgc cggccggccc
     7261 tcaatcccgc acgcaatcca caaagtaatg gatgcgcccg tccttcacct ccttcaccag
     7321 accatgcacg tcggtctcga aaccagggaa gcgctcgttg aactcacggg cgaactgaag
     7381 gtagcgcacc acggtgcggt tgaagcgctc gccggggatc agcagcggga tgcccggcgg
     7441 gtagggcgtg aggagcacgc tggtgacgcg gccttcgagt gcgtcgatgg gcacccgctc
     7501 gatctcccgg tgggccatct tggcgaaggc gtcagccggc ttcatggcgg ggatcatgtc
     7561 cgacaggtac atctcggtgg tcaggcgggc cacgtcgttg gcgcggtaca ccgagtggat
     7621 ctcgcggcac aggtcacgca ggccgacgcg ctcgtagcgc gggttcttct gcacgaactc
     7681 gggcagcacc ttccacaggg gctggttctt gtcgtagtcg tccttgaact gctggagctc
     7741 ggtcaccatg ctgttccagc ggcccttggt gatgccgatg gtgaacatga tgaagaagga
     7801 gtacaggccg cacttctcga cgatcacgcc atgttcggcc aggtacttgg tgacgatggc
     7861 cgccgggatg ccgcgctcgg cgaattcgcc gtccacgtcc aggcccgggg tgatgatggt
     7921 ggccttgatc gggtcgagca tgttgaaccc ttcggccagg cggccgaaac catgccatcg
     7981 ctcgcccggc ttgagcatcc acgcctcgcg ctcctcgatg ccctcttcgg acaggtcgtc
     8041 cggcccccac accttgaacc accagtcggc gccccactcc tcgtccacct tgcgcatggc
     8101 gcggcggaaa tcaagggcct cggcgatgga ttcctccacc agcgcggtgc cgcccggctc
     8161 ttccatcatg gccgccgcca cgtcgcagga cgcgatgatg gcgtactggg gcgaggtgga
     8221 ggtgtgcatc aggtaggctt cgttgaagac gtcccggtcc aggcgctggt gttcggcatc
     8281 ctgcacgagg atctgggatg cctgggacag gcccgccagc agcttgtggg tggactgggt
     8341 ggagaacacc atcgattcct tgcagcgcgg acggtcggcg ccgatggcgt ggtagtcgcc
     8401 gtagaaatcg tggaaggccg cgtggggcaa ccaggcttcg tcgaaatgca gggtgtcgat
     8461 gaggccgtcc agctcttcct tgatcgcctc cacgttgtag agcacgccgt cgtaggtgga
     8521 ctgggtgatg gtcagcaccc gaggcttggc gttcttgtcg gtgatgaagg gattggcggc
     8581 gatgcgcttc tggatgttct cccacgcgaa ttcgctcttc gggatggggc cgatgatccc
     8641 gtagttgttg cgcgtgggca tcaggaacac cgggatcgcg ccggtcatca tgatggcgtg
     8701 gagcaccgac ttgtggcagt tgcggtccac caccaccacg tcgccggggg ccacggtgga
     8761 gtgccacacg atcttgttgg aggtggacgt gccgttggtg acgaagtaca ggtgatcgca
     8821 gttgaagatg cgcgccgcgt tgcgctccga ggccgccacc gggccggtgt ggtcgaggag
     8881 ctggccgagc tcctcgaccg cgttgcacac gtcggcacgc agcatgttct cgccgaagaa
     8941 ctggtggaac atctgcccga ccgggctctt caggaaggcc acgccgccgg aatggccggg
     9001 gcagtgccac gaataggagc cgtcggaggc gtaatgggtg agcgcccgga agaaaggcgg
     9061 cggcagcgca tccatgtagg cgcgggcctc gcgcaccacg ttgcgggcga tgaactccgg
     9121 cgtgtcctcg tacatgtgga tgaagccgtg gagctctcgc agcacatcgt tggggatgtg
     9181 ccgcgacgtg cgcgtctcgc cgtgcaggaa gatggggatg tccgcattgc gcaggcggat
     9241 ctcgctgacg aaggcgcgca ggtccgcgat ggccttggca gcggcctccg gcgacgagaa
     9301 ctcctcgtcg tcgatggaca ggatgaaggt ggaggcgcgg ctctgctgct gggcgaagga
     9361 cgccaggtcg ccgtagctgg tcacgcccac cacctgcagg ccttccttct cgatggcttc
     9421 cgccagggcg cggatgccca gcccggacgt gttttccgaa cgaaagtcct cgtcgatgac
     9481 gatgaccgga aaattgaagc gcatgctcga ctcctcgatg tccgccgcgc ggacggctca
     9541 acaaaaaagg cgtgggcgga tctgcgtggc tgcgcgcagg atccgcccct tatcggactg
     9601 tagccgaccc gtgtttcaga ttttgggcag ggtgacgccg gtctgcccca ggtatttgcc
     9661 accgcggtca cggtaagacg tgtcacagat ctcgtccgac tcgaagaaca gcatctgggc
     9721 gacgccctcg ttggcgtaga tcttggcggg cagcggcgtg gtgttggaaa actccagggt
     9781 cacgtggcct tcccattccg gctccagcgg cgtgacgttc acaatgatgc cgcagcgggc
     9841 gtaggtggac ttgcccaggc acacggtgag cacgctgcgg gggatgcgga agtactcgac
     9901 ggtgcgggcc agggcgaagg agttcggcgg aatgatgcac acgtcgccgg tgaaatccac
     9961 gaagttgcgc tcgtcgaagg ccttgggatc gacgatggtg gagttgatgt tcgtgaagat
    10021 cttgaattcg ttggccacgc gcacgtcgta gccatagctg gaggtgccgt aggagacgat
    10081 cttgccgcct gcgttctggc gcaccagttc gggggcgaag ggttcgatca tcttcgcatc
    10141 gccttccgcc atgcgacgaa tccacttgtc cgacttgatg ctcacgctgg ctgcccccgc
    10201 acaaaaacgc gcattctaca tcccttcgtc tggaaaaccg gccaaaaaat cggggcctta
    10261 cggcccctcg acccgcccgc cgcacgggcc cgcatcagcg tccggcgtcc ttgacgtcgt
    10321 cgtcctttac ggcatcgtag acgcccttgc ccaccttgaa gggcaccttg accacctcga
    10381 cgcccacatc taccgccgtg ccgaccacgg tgccgacaac acagcccgac agactggccg
    10441 ccaggacgac ggccggtccc cacgcacgca ggcgccgcat caggtgttct gcaccacgat
    10501 gctggggaac ttgtgactca tgtccttctg ccgctcggcc acggcaatcg ccaccttgcg
    10561 ggcaatctcc ttgtagacct gcgcgacgcg cccatccgga tcggccgcca cggtcggcgt
    10621 accgccgtcc gcctcgcgac ggatctggat gtccagcggc agggcgccga ggaagggcac
    10681 gccatagtcc tcacccatcg tcgcaccgcc gcccgaaccg aagatgtgct cttcgtggcc
    10741 gcactgggag cagatgtgga tgctcatgtt ctcgacgatg ccgaggatcg gcacgcccac
    10801 cttctcgaac atcttgaggc ccttgcgggc gtccagcagg gcgatgtcct ggggcgtggt
    10861 gacgatcacc gcgccggtca ccggcacgct ctgggagagg gtcagctgga tgtcgccggt
    10921 gcccggcggc atatcaatga ccaggtagtc cagatcgcgc cagttggtct ccttgaggag
    10981 ctggttgagg gcctgggtgg ccatcgggcc acgccacacc atgggcgtct ccacgtccac
    11041 caggaagccg atggacatca cctgcaggcc gtgggcctcc agcggctcca tggtcttgcc
    11101 gtcgcgggat tccggctggg aatcgccgat gcccagcatc tggggctggg acgggccgta
    11161 gatgtccgcg tcgaggatgc ccaccgtggc gccttcagcc gcgagggcca gggccaggtt
    11221 cgcggacgtg gtgctcttgc ccacgccgcc cttgccggag gccacggcaa tgatgttctt
    11281 cacgccgggc aacagcttca cgccctgctg cacggcgtgg gcgacgatcc gggtgaatac
    11341 attggcgctc acattgccca cgcccggcac ggacttcgca caggcgatca ccgcctggcg
    11401 gatcgtgtcg atctggctcg cggcgggata gcccagttcg atatcgaagg acacgtcggc
    11461 accgtgcagg cgcaggttct tgacggcgcg ggacgtcaca tagtccttgc cggtgttggg
    11521 gtcggcgtac tgccggaggc tgtcgagaat gctctgttct gtgaggctca tgggcgcgca
    11581 ggccgcgttg cggcgattcg gaaagggtgg atgatgcggg cacgcctccc catccggagg
    11641 cgcaaacccg agtgatttag tccagtttac ccaaaagcgg gccggtccgc gatgggctcg
    11701 tcgcttggta gaatgcagga ttcgatccac cgatccaggc ctgtttcatg accacgcgca
    11761 agatcctcgt caccaacgcc ctgccttacg ccaacggcga catccacctc ggccacctcg
    11821 tcggctacat tcaggccgac atctgggtgc gctaccagcg catgcgcggc cacacggtgc
    11881 actacgtctg tgccgacgac acccacggca cgccggtcat gctgcgggcc gaaaaggaag
    11941 gcctcacgcc ggagcagctc atcgcccgcg tccatggcga acacctgcgc gacttcaccg
    12001 acttcggcgt ggccttcgac cactatcatt ccacccacag cagcgagaac cgctggtacg
    12061 cggaagacat ctacacccgc ctgcgggaca gcgcgcgcct cattgagacc cgcgccatcg
    12121 aacagttcta cgacccggtc aaggagatgt tcctgcccga ccgcttcatc aagggcgaat
    12181 gtcccaagtg cggcgcccag gaccagtacg gcgacaactg cgaggtatgc ggcgctgcgt
    12241 atgcgcccac cgatctcaag cacccccgct ccgccgtgtc cggcgccgaa cctgtgctca
    12301 agacctccga gcactatttc ttccgcctct ccgatccccg cgcggtggaa ttcctgcggg
    12361 aatggaccca gggcaccaca cccgaaggcg aacgccgcct gcaggccgag gcggccaaca
    12421 agatgaagga gtggctcggc gactccggcg agaacgcgct ctccgactgg gacatctccc
    12481 gcgacgcacc ctacttcggc ttcgagatcc ccggcgcgcc gggcaagtac ttctacgtgt
    12541 ggctggacgc ccccatcggc tacttcgcca gcttccgcca cctggccatc ctgcgtggcg
    12601 acatcgacgt tgccgactac accgaagccg gccccgcagc cgccaccggc accgaactga
    12661 tccacttcat cggcaaggac atcctctact tccacgccct cttctggccc gccatgctcc
    12721 acttcgccgg ctaccgcacg cccacccagt tgtgcgtgaa cggtttcctc accgtcgatg
    12781 gcgccaagat gagcaagagc cgcggcacct tcatcaccgc ccgttcctac accg
//
LOCUS       DAOQPA010000102        12498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2584_length_12498_cov_16.586595, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000102 DAOQPA010000000
VERSION     DAOQPA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2584_length_12498_cov_16.586595"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PKX01_13160"
     CDS             <1..1707
                     /locus_tag="PKX01_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMZ77125.1"
                     /translation="EAIQTPPSGTWPSFGRDYSNQRFSPLTQIDAGNVHRLARAWTYR
                     SGIQATFQATPIVVDRTMYLSLPFNHVVALDARTGEERWRYVHTLMPGRTPCCGPSNR
                     GVAVADGKVFMGTVDGRLIALDQRSGARLWDVDLLERTPTQTESVSRIAPDDPLAAAR
                     FVGSTGAGIAMAPVVYQGKVLIGITGVGFGLHLDSSAENPLTVVGLPGNYGRSGFLAA
                     FDAATGARLWTFDTVRADAWEGDYRSHTPDGHALPRDIPGEKAAAARYPSAWKFGGGA
                     AWSSPAIDTLRGLLYFGTGNPSPNMEDSSRPGDNLYTTSLVALEIETGALRWHFQQVP
                     HDIWGYDVASPAVLFDFERDGRRIPAVAQASKLGWLYIHDRETGALLLKSDAFVPQHN
                     LFQRPTTAGLRVHPGVTGGVNWSPMALDATAGLAYVAAMHWPVIYTLHEKPAANGRPA
                     SRYTSMDPVKDERWGVLAAIDLKTGHVRWQHRTREPLIGGTLATAGNLVFVGEGTGEL
                     DAFDATRGTLLWQHTHDAGVNAPPITYAIDGVQYVAVVVGGNHLFGFTQGDQVAVYAL
                     GR"
     gene            complement(1729..2277)
                     /locus_tag="PKX01_13165"
     CDS             complement(1729..2277)
                     /locus_tag="PKX01_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ77126.1"
                     /translation="MPSTAPGLLTPRLHLRPWRAEDLSPFAALNADPEVMRHFPATLE
                     RAASDALARRIDQLIAAQGWGFWAAELRASGAFIGFIGLHRPTHLPFSPCIEVGWRLA
                     RPFWGLGLATEGARAALEFAFHTLAADEVVAFTALSNQRSEAVMRRLGMQRDAAPFAH
                     PDLPDGHPLQAHVLYRIRRPGA"
     gene            complement(2308..2958)
                     /locus_tag="PKX01_13170"
     CDS             complement(2308..2958)
                     /locus_tag="PKX01_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77127.1"
                     /translation="MLKFAAALAGGLALLLLPRLGRLRTRYDTAALHPLPGDAPADDP
                     ALVQRLTAWARDGMGCGATVLPTAQPRVPRPLAVAWADAAAADSVQHAAYRLAGYHQL
                     DTRSRLGGLLYRVGVQLRPLLWCVPLAADAPWDDAWFAAGSTLPLDALRAWRPRRPTV
                     IVVADATQVTALVDALTGTPSHGGHALRVVGLLPAGAVPPALPGDVPVCDLRATPR"
     gene            complement(2952..3809)
                     /locus_tag="PKX01_13175"
     CDS             complement(2952..3809)
                     /locus_tag="PKX01_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ77128.1"
                     /translation="MAKRKPAAGTPAPVAHPDLRPGQSVELLQALHILTRDGQLNQDS
                     RRKLKQVYHLHQFIEPLLAEVLAERGDLSLADHGAGKSYLGFILYDLFLKQQAAGTVY
                     GIETRRELVEHSRALAERLGFARMHFLDVSVADSTHAAELPARIDVVTALHACNTATD
                     DAIQFALAKDARFLVLVPCCQAEVAAVLRQNKNASFGLTPLSEIWRHPLHTREFGSQL
                     TNALRCLQLEAHGYQVTVTELVGWEHSLKNELIIARRTGQPRRNAMARIEQILDQLNL
                     TELRSRFAC"
     gene            complement(3796..4359)
                     /locus_tag="PKX01_13180"
     CDS             complement(3796..4359)
                     /locus_tag="PKX01_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HMZ77129.1"
                     /translation="MNHEEVIAATRAWIEKAVIGLNLCPFAKAVYVKDQVRIVVSDAR
                     HIDGFLEDLDRELDHLAAVDPEVTDTTLLVHPTLFPDFLDFNDLTQIADEAVSEHELD
                     GVLQIASFHPDFQFEGTEADDITNYTNRSPYPTLHLIREASIDRAVEAFPEAEMIYER
                     NMETLRTLGIAGWKALGLSTPKRDGEA"
     gene            4442..5362
                     /gene="ylqF"
                     /locus_tag="PKX01_13185"
     CDS             4442..5362
                     /gene="ylqF"
                     /locus_tag="PKX01_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HMZ77130.1"
                     /translation="MSIQWFPGHMTSARKKAAESMERIDVVIEVVDARLPEASTNPMI
                     RELRAHRQRPCLKILNKADLADPAVTARWIAWYNAQKGVSAVALSCKKPGDVAKVVGL
                     CRTLAPHRNDRLKPLRMMIMGIPNVGKSTLMNALLKKRVAKVGDEPAVTKSQQAIDLS
                     PTASLVDTPGMMWPKILHDSDGFMLAASHAIGRNAVIDEEVANFLGGLLLQRYPALLV
                     KRYGFPVDGLDGPALVEAIGRRRGCLQKGGGLDLEKASLILLQDYRDGALGRVSLETP
                     ETRAVMLQAYAEALAQKAQAAEDSAENALE"
     gene            5480..7681
                     /locus_tag="PKX01_13190"
     CDS             5480..7681
                     /locus_tag="PKX01_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77131.1"
                     /translation="MTRPNNAALTRKKIQLGFLCGACLVAALVSGWLWRSWQDARTYE
                     FSHLSTIAELTGNSLDQYFSRHEHQLRVLGRDILREAAEPDAFPALLARFKESEPDCL
                     FLTLSAADGTLIATDFTGPRETLPNTQDLPGRRAAYEELARQDRLNIGRPFEGRLMKA
                     WIMPLRLGVRDDTGALRYVISCTLPLSRQQTFWQNLSLPPGSSFGLLRDDAYLVSRYP
                     PAPDGNLQDTYGKPRDGALVMHLREAGFPHRGIVYGYNSVLRKDTLFAYQRLGHYPLT
                     VFVTTPGDGALNRWLDQLYLPSFLALISLVVSVLMYRTIARRIAVSEELERYQGRLEE
                     LVEQRTHELADAKSAAETASQAKSQFLANMSHEFRTPLNGLMGMLRLARGRMRDPAGL
                     EQLDKAAIAATHLATIINDVLDVSKIEAAQLQLEAIEFFLPPVVDTAFSMNTARAAAK
                     GVSLRSELTPAVAAQAYVGDPLRLGQILINLVSNAVKFTERGSVTVLARLLDHTDSRA
                     TLRFEVIDTGIGIPRDQQDRLFSAFEQADSSTTRKYGGTGLGLAICKRLVELMGGRIG
                     LDSEIGRGCTVWFEITLPVTAGRPAADAAPDLDALEAEIRARHAGKIVLVVEDEPINQ
                     EITRCNLEDIGLVVQVAGNGQEAVAMAGQTDYALILMDMQMPVMNGLDATQAIRRGAR
                     NARTPILAMTANAFVEDRDLCLAAGMNGHIAKPVELAVLYREVLAWLGSDA"
     gene            7917..9347
                     /locus_tag="PKX01_13195"
     CDS             7917..9347
                     /locus_tag="PKX01_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ77132.1"
                     /translation="MAPVPKLIVLLLGGILAGCAVPRVGPDYQTPQAELPAVWTATLP
                     DASANASDATALARWWTQFDDPALDRLVGEALSQGLDVRLAQARVQQARASRQLAEAG
                     LSPTVAASAGGTRSKSSQAVAAVPTRTLYDAGFDASWELDLFGGTRRGIEAAQADVAA
                     SQASLEQVRVTLVAEVARNYVDLRAAQQRIRIARDNLASQRETLQITEWRYQAGLARA
                     SEVEQARTAEAQTRAGIPDLEIDLVAAQNRLAVLLGRAPGSLHDQLAAVKPLPALPTQ
                     LATGIPADVLRQRPDIVAAERTLAAETARVGQKLAQRFPSLSLSASFGWQAYSTAALG
                     GAANAARALGATLATTLFDGGRLRSQVDVQSAVQEQALVAYQSTVLTALEEVENALKS
                     HAAARQRFAARQAAAEAARNAADLSRKLYQAGLADFQQVLETQRTQLSAEDSLATADA
                     SLRTSLISLYKALGGGWDAAARNGTS"
     gene            9344..10618
                     /locus_tag="PKX01_13200"
     CDS             9344..10618
                     /locus_tag="PKX01_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77133.1"
                     /translation="MNDTSSSDSLKQLLDQAPRGGRGVAWRRWLWAGGLVLVLGGVFV
                     ALRGDKGAGGHYLTEAAERGALVVTVSATGTLQPTTSVDVGSEQSGTLAEVLVQENDR
                     VKQGQLLARLDLAKLQDAVSKSRAALAAAEASVAQARATVAESRASLARMRQVAELSG
                     GKVPSKTELETAEATLERAVANEASARATVMQAQAALKTDETNLSKGTIRSPVNGVVL
                     SRKVEPGQSVVAAMTIPVLFTLAEDLTAMELQVKVDEADVSSVRLGQPASFTVAAWPA
                     RTFPATIERVGIGSTITDNVVTYKTVLAVKNDDLALRPGMTATASIVTAKRDDALLVP
                     NAALRFTPPARGADARQGSFVSSLLPRPPSETKHATAAPAGSPQVWVLGDDGPRAVPV
                     KTGVSNGRQTEILGGELKPGMAVITEYQEGRP"
     gene            10615..11331
                     /locus_tag="PKX01_13205"
     CDS             10615..11331
                     /locus_tag="PKX01_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77134.1"
                     /translation="MSAERAEPLIRLVGVTKTYGEGGVAYQALRGIDLEVAAGEFVAI
                     MGPSGSGKSTVMNILGCLDTPTSGAYLFRGIHVEQLDRNQRALLRRHSLGFVFQGFNL
                     LARTSAQENVELPLLYRGETAPARHAAARAALDRVGLAGWEPHTPAELSGGQQQRVAI
                     ARAIVTQPDVLLADEPTGNLDSQRSHEIMALLVELNRKQGITVLMVTHEPDMAAYARR
                     IVTFVDGRVARDEAVPERAL"
     gene            11328..>12498
                     /locus_tag="PKX01_13210"
     CDS             11328..>12498
                     /locus_tag="PKX01_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77135.1"
                     /translation="MSSVLVSAFLLALREIRRNLMRSFLTVLGIVIGVAAVVTMVTLG
                     NGATRAVSDQISSLGSNLLMVRPGQRLGPGRDSAGAPAFKRADAEALRTQLTGMDEVV
                     PVAGSSVTVVAGNNNWSTTVSGTTNGYLKAGNWKIASGRAFQDDEERAGKSVCVIGAT
                     VRRELFGTRNPLGDEIRVKTFACQVVGLLAAKGQGAMGMDQDDTVLMPLATVQRRLTG
                     TQDVSTLMVSLHDGVDAKPVMAQIRALLRERRKLADNEADNFNVMDTKQIAETLSGTI
                     RTMTGLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGALEHEVLLQFLIEA
                     VVLSACGGLIGILLALLACVALSALLSMPFLFNTGINLLAFLFSALIGVVFGYMPA"
BASE COUNT         1930 a         4527 c         4242 g         1799 t
ORIGIN      
        1 gaggccatcc agacgccccc gtccggcacc tggccgagct tcgggcggga ttacagcaac
       61 cagcgcttct cgcccttgac gcagatcgac gccggcaacg tgcatcggct cgcgcgcgca
      121 tggacctacc ggagcggcat ccaggccacc tttcaggcga cgcccatcgt ggtggaccgg
      181 accatgtacc tgtccctgcc gttcaaccac gtggtggccc tggatgcgcg caccggcgaa
      241 gagcgctggc gttacgtgca caccctcatg cctggcagga cgccgtgctg cgggccgtcg
      301 aaccggggcg tggcggtggc tgacggcaag gtcttcatgg gcaccgtgga cggtcgcctg
      361 attgccctcg atcagcgcag cggtgccagg ctgtgggacg tggacttgct ggaaaggacg
      421 ccgacccaga ccgaaagcgt gtcgaggatc gcgccggacg acccgctggc ggcggcgcgc
      481 ttcgtcggct ccaccggcgc gggcatcgcc atggccccgg tggtctacca gggcaaggtg
      541 ctgatcggca tcaccggtgt cggcttcggc ctgcacctgg attcgagcgc cgaaaacccc
      601 ctgacggtgg tcggtctgcc cggtaactac gggcgctcgg gctttctcgc cgctttcgac
      661 gccgccaccg gggcgcgcct gtggaccttc gacacggtgc gcgccgacgc ctgggaaggg
      721 gactatcgca gccatacgcc ggacggccac gcgctgccgc gggacatccc cggcgaaaag
      781 gccgccgcgg cccgataccc gtcggcctgg aaattcggcg gcggcgcggc ctggtcctca
      841 cccgccatcg acaccctgcg tggcctgctg tatttcggca ccggcaatcc ctcgcccaac
      901 atggaggaca gctcccgccc cggcgacaac ctctacacca cctcgctggt ggccctggag
      961 atcgagaccg gagccctgcg ctggcatttc cagcaggtgc cccacgacat ctggggctac
     1021 gacgtggcca gcccggcggt gctcttcgac ttcgagcggg acggccggcg catccccgcg
     1081 gtggcccagg ccagcaagct gggctggctc tacatccacg accgggagac cggcgcgctg
     1141 ctgctcaagt ccgatgcctt cgtgccccag cacaacctgt tccagcgccc cacgacggcg
     1201 ggcctgcgcg tccatccggg cgtgaccggc ggggtgaact ggtcgcccat ggccctcgac
     1261 gcgacggccg gcttggccta cgtggccgcc atgcactggc cggtgatcta caccctccac
     1321 gaaaagcctg ccgccaacgg acgtcccgcc tcacgctaca cctcgatgga tccggtcaag
     1381 gacgagcgct ggggcgtgct cgccgccatc gacctgaaga ccggccacgt gcgctggcag
     1441 catcgcaccc gggaacccct catcggcggc accctggcga cggccggcaa cctggtgttc
     1501 gtcggcgaag gcaccggcga gctggacgcc ttcgatgcca cccgcggcac cctgctgtgg
     1561 cagcacaccc acgacgccgg cgtcaacgcg ccgcccatca cctacgccat cgacggcgtg
     1621 cagtacgtgg ccgtggtggt cggcggcaat cacctcttcg gattcaccca gggcgatcag
     1681 gtggcggtgt acgccctggg tcggtagcgc cgcccgcgcc gccacccttc acgcacccgg
     1741 tcggcgaatc cggtagagga cgtgcgcctg cagcggatgc ccgtccggca ggtccgggtg
     1801 ggcgaagggc gcggcgtcgc gctgcatgcc caggcgtcgc atcaccgctt cggagcgctg
     1861 gttgctcagg gccgtgaagg ccaccacctc gtccgccgcc agcgtgtgga aggcaaattc
     1921 cagcgcggcg cgggcgcctt ccgtggccag accgagcccc cagaaaggcc gcgccagacg
     1981 ccagcccacc tcgatgcagg gggaaaaggg caggtgcgtc gggcggtgga gcccgatgaa
     2041 accgatgaac gctccgctcg cccgcagctc cgcggcccag aagccccaac cctgggcggc
     2101 gatcaactga tcgatgcgcc gcgccagggc gtcgctggcg gcccgctcca gcgtggctgg
     2161 gaagtgccgc atcacctccg ggtcggcgtt gagggcggcg aatggtgaca gatcctccgc
     2221 ccgccagggg cgcagatgca ggcggggcgt gagcagtccg ggcgcggtgg aaggcatgga
     2281 gcgcaggccg gtggattgcg ggtggcgtca gcgtggtgtc gcgcgcaggt cgcagaccgg
     2341 cacgtcgccg ggcagggcgg gcggcacggc gccggcgggc agcagtccga ccacgcgcag
     2401 ggcgtggccg ccgtgggacg gggtgccggt cagggcgtcc accagcgccg tcacctgcgt
     2461 ggcatcggcg acgacgatca cggtgggacg acgcggccgc caggcgcgca gggcgtcgag
     2521 gggcagcgtt gatccggcgg cgaaccaggc gtcgtcccag ggcgcgtcgg ccgccagggg
     2581 cacgcaccac agcagggggc ggagctgcac gcccacgcga tacagcaaac cgcccaggcg
     2641 gctgcgggtg tcgagctggt gatagccggc gagacggtag gccgcgtgct gcacgctgtc
     2701 cgccgcggcg gcatcggccc aggccaccgc gaggggacgc ggcacgcggg gctgcgcggt
     2761 gggcagcacg gttgcgccgc agcccatgcc gtcccgcgcc caggccgtca ggcgctgcac
     2821 gagggccggg tcgtcggccg gggcgtcgcc gggcagggga tgaagggccg ccgtgtcgta
     2881 acgggtgcgc aggcggccga ggcggggcag caggagcagg gcaaggccgc ccgccagtgc
     2941 cgcggcaaac ttcaacatgc gaagcggctc cgcagctcgg tgagattgag ctggtcgagg
     3001 atctgctcga tgcgcgccat cgcgttgcgg cggggctggc cggtgcggcg ggcgatgatc
     3061 agctcgttct tgagggagtg ttcccagccc accagctcgg tcaccgtcac ctggtagccg
     3121 tgggcttcca gttgcaggca gcgcagggcg ttggtgagct ggctgccgaa ttcgcgggtg
     3181 tgcaggggat ggcgccagat ctccgagagg ggcgtgaggc cgaagctggc gttcttgttc
     3241 tggcgcagca cggcggcgac ttccgcctgg cagcagggca ccagcaccag gaagcgggcg
     3301 tccttggcca gggcgaactg gatcgcgtcg tcggtggcgg tattgcaggc gtgcagggcg
     3361 gtgaccacgt cgatgcgcgc cgggagttcg gcggcgtggg tggaatcggc cacgctcacg
     3421 tccaggaagt gcatgcgcgc aaagccgagg cgctccgcca gggcgcggga gtgttccacc
     3481 agctcgcggc gggtctcgat gccgtacacc gtgccggccg cctgctgctt gaggaacagg
     3541 tcgtaaagga tgaagcccag gtaggacttg cctgcgccgt ggtcggccag actcaggtcg
     3601 ccgcgctcgg ccagcacctc ggccagcagc ggctcgatga actggtgcag gtgatagacc
     3661 tgtttgagct tgcgccggct gtcctggttg agctggccgt cgcgggtgag gatgtggagg
     3721 gcctggagca gctcgacgga ctgccccggg cgcaggtccg gatgggcgac gggggccggc
     3781 gtgccggcag cgggcttacg cttcgccatc gcgcttgggc gtggacaggc ccagggcctt
     3841 ccagccggcg atgcccagcg tgcgcagggt ctccatgttg cgctcgtaga tcatctccgc
     3901 ctccggaaag gcttccacgg cccggtcgat gctcgcttcg cggatcaggt gcagggtggg
     3961 gtagggcgag cggttggtgt aattggtgat gtcgtccgcc tcggtgccct cgaactggaa
     4021 atccgggtgg aagctggcga tctgcaggac gccgtccagt tcgtgctcgc tgaccgcctc
     4081 gtcggcgatc tgggtgaggt cgttgaagtc caggaaatcc gggaacaacg tgggatgcac
     4141 cagcagcgtg gtgtccgtca cctccggatc gaccgccgcc aggtggtcca gctcccggtc
     4201 caggtcttcc agaaagccgt cgatgtggcg agcgtcgctt accacgatgc ggacctggtc
     4261 cttcacatag accgctttgg cgaaggggca caggttgagg ccgatgacgg ccttctcgat
     4321 ccacgcccgc gtggcggcga tgacttcttc gtgattcatg gctggggttt ccggctgatt
     4381 cctgcgaggg ccgccatttt gccctgataa tgtcgccctt tcattgattc agcgcacgcc
     4441 catgtccatc cagtggttcc ccggtcacat gacctccgcc cgcaagaagg ccgcggagag
     4501 catggaacgc atcgacgtgg tcatcgaggt cgtggacgcc cgcctgccgg aggccagcac
     4561 caaccccatg atccgcgaac tgcgcgccca ccgccagcgc ccctgcctga agatcctcaa
     4621 caaggccgac ctggccgacc cggccgtcac cgcccgctgg atcgcctggt acaacgccca
     4681 gaagggcgtc tccgcggtgg ccctgtcgtg caagaagccc ggcgacgtgg ccaaggtggt
     4741 gggcctgtgc cggacattgg cgccgcatcg caacgaccgc ctcaagcccc tgcgcatgat
     4801 gatcatgggc atccccaacg tgggcaaatc cacgctgatg aacgccctgc tcaagaagcg
     4861 cgtcgccaag gtgggcgacg agcccgccgt caccaagagc cagcaggcca tcgacctgtc
     4921 gcccaccgcg tcgttggtgg atacgccggg catgatgtgg cccaagatcc tccacgattc
     4981 cgacggcttc atgctcgccg ccagccacgc catcggccgc aacgccgtca tcgacgaaga
     5041 ggtcgccaac ttcctcggcg gcctgctgct gcaacgctac ccggcgctgc tggtcaaacg
     5101 ttatgggttc cccgtggacg gcctggacgg cccggccctc gtcgaagcca tcggccgccg
     5161 ccgcggctgt ctgcagaagg gcggcgggct cgacctggag aaggcctccc tgatcctgct
     5221 gcaggattac cgcgacggcg ccctcgggcg cgtcagcctg gagaccccgg agacccgcgc
     5281 cgtcatgctc caggcctatg ccgaggcgct ggcgcagaaa gctcaagcgg cggaagatag
     5341 cgccgaaaac gccctggagt aggtgcggag gctgccgtca tcgggggccg acccgatgcc
     5401 tgcgggccct catccggcgg accgcatccg ccgccctcgc agccccacgg ggcttgaatc
     5461 tcggaaggca tgccgcgcca tgacccgtcc caacaacgcc gccctcaccc gcaagaagat
     5521 ccagctcggc ttcctgtgcg gcgcctgcct ggtggcagcc ctggtctcgg gctggctgtg
     5581 gcgctcgtgg caggacgcgc ggacctacga gttctcccat ctctccacca tcgccgagct
     5641 caccggcaat tccctcgacc agtacttctc gcgccacgag caccagttgc gtgtgctggg
     5701 gcgcgacatc ctgcgcgagg cggccgaacc cgacgccttc ccggccctgc tggcgcgctt
     5761 caaggagagc gagcccgact gcctcttcct caccctctcc gcggctgacg gcaccctgat
     5821 tgccaccgat ttcaccggac cccgcgagac cctccccaat acccaggatc tgcccggacg
     5881 ccgtgccgcg tacgaggaac tggcccgtca ggaccgcctg aacatcggcc ggccctttga
     5941 agggcgcctc atgaaggcgt ggatcatgcc gctgcggctg ggtgtgcgcg acgacaccgg
     6001 cgcactgcgc tacgtcatca gctgcaccct gccgctgagc cgtcagcaga ccttctggca
     6061 gaacctcagc ctgccaccgg gctcgtcttt tggcctgctg cgggacgacg cctatctcgt
     6121 cagccgctat ccgccggccc ccgacggcaa cctgcaggac acctacggca agccccgcga
     6181 cggtgcgctg gtcatgcacc tgcgggaggc cggcttcccc caccggggca tcgtctatgg
     6241 ctacaacagc gtcctccgca aggacacgct gttcgcctac cagcgcctgg gccactaccc
     6301 cctcaccgtg ttcgtcacca cgccgggcga cggcgccctc aaccgctggc tggaccagct
     6361 ctacctgccc agcttcctgg ccctcatctc cctcgtcgtc tcggtgctca tgtatcgcac
     6421 catcgcccgg cgcatcgccg tctcggaaga gctggagcgt taccagggcc gcctggaaga
     6481 gctggtcgag cagcgcaccc acgaactcgc cgacgccaaa tccgccgccg agaccgcgag
     6541 ccaggccaag agccagttcc tcgccaacat gagccacgaa ttccgcacgc ccctcaacgg
     6601 cctgatgggc atgctgcgcc tcgcccgcgg ccgcatgcgc gaccccgccg gcctcgaaca
     6661 gctcgacaag gccgccatcg ccgccaccca cctggccacc atcatcaacg acgtcctcga
     6721 cgtctccaag atcgaagccg cccagctcca gctcgaagcc atcgagttct tcctgcctcc
     6781 ggtggtggac accgccttca gcatgaacac cgcgcgggcc gccgccaagg gcgtgagcct
     6841 gcgcagcgag ctcacacctg ccgtcgccgc ccaggcctac gtgggcgatc ccctgcgcct
     6901 cgggcagatc ctcatcaacc tcgtctccaa cgccgtcaaa ttcacggagc gaggctcggt
     6961 gacggtcctg gcgcggctgc tggaccacac cgacagccgt gcgacgctgc gtttcgaggt
     7021 catcgacacc ggcatcggca ttccccgcga ccagcaggac cgcctcttca gcgcctttga
     7081 acaggccgac agttccacga cccgcaagta cggcggcacc ggtctcggtc tcgccatctg
     7141 caagcgcctc gttgagttga tgggtggccg catcggtctg gacagtgaaa tcggccgggg
     7201 ctgcaccgtg tggttcgaga tcaccctccc tgtcaccgcc ggcaggcccg cggcggacgc
     7261 cgcccccgac ctggacgccc tcgaagccga aatccgtgcc cgtcacgccg gcaagatcgt
     7321 gctggtcgtc gaggacgaac ccatcaacca ggaaatcacc cgctgcaatc tggaagacat
     7381 cggcctcgtc gtccaggtcg ccggcaacgg ccaggaagcc gtcgccatgg ccgggcagac
     7441 cgactacgcc ctgatcctga tggacatgca gatgcccgtc atgaacggcc tcgacgccac
     7501 ccaggcgatc cgccgcggcg cccgcaacgc ccgcaccccc atcctcgcca tgaccgccaa
     7561 cgccttcgtc gaagaccgcg acctatgcct cgccgcgggc atgaacgggc acatcgccaa
     7621 gccggtggag ctggcggtgc tgtatcgcga ggtgctggcg tggctgggtt cggacgcgtg
     7681 agggcggggc gtcgccggcg ctcgtccgtt cccgcggagt gttccggatc gaaaggctga
     7741 gtgtgccggc gtctcggcgc gtcgcgtgcg ccgcccgagc ctgcatgtcc gcgctttcca
     7801 gtccgtcgtc gcgcgccatg tccggttcga atccccgccg taaagttggg taaaagtccc
     7861 gcccccgcag accccgcccc ctagaatcgc cggcgtgtgc tgaatcgctg gtcttcatgg
     7921 ctcccgttcc aaaattgatc gtgctcctgc tggggggaat cctggcgggc tgtgccgtgc
     7981 ccagggtggg gccggactat cagacgccgc aggcagaact gcccgccgtg tggaccgcga
     8041 cgcttccgga cgcgtctgcg aacgcctccg acgccaccgc cctggcgcgc tggtggaccc
     8101 agttcgacga tcccgcgctc gaccgcctcg tcggcgaggc gttgagccag gggctcgacg
     8161 tccggctggc ccaggcgcgg gtgcagcagg cgcgggcgag tcgccaactg gcggaggcgg
     8221 ggctgtctcc caccgttgcc gcgtcggcgg gcggcacgcg cagcaagagc tcccaggcgg
     8281 tggcggcggt gccgacgcgc acgctctacg atgcgggctt cgacgcgagc tgggagctcg
     8341 atctcttcgg cggcacgcgt cgcggcatcg aggcggccca ggcggacgtg gcggcgagcc
     8401 aggcgagcct ggaacaggtg cgggtgacgc tggtcgccga agtggcgcgc aattacgtgg
     8461 acctccgcgc cgcccagcag cgcatccgca tcgcccgcga caatctggcg agccagcgcg
     8521 agaccctgca gatcaccgag tggcgctatc aggccgggct ggcgcgggcc agcgaggtgg
     8581 agcaggcgcg caccgccgag gcccagacgc gggcaggcat tccggatctg gagatcgacc
     8641 tcgtcgccgc gcagaaccgg ctcgccgtgc tcctcgggcg tgcgccgggc agcctgcacg
     8701 atcaactggc ggccgtgaag ccgctgcccg ccttgcccac gcagctcgcc accggcattc
     8761 cggcggacgt gctgcgccag cgcccggaca tcgtggccgc cgagcgcacc ctggcggcgg
     8821 aaacggcgcg ggtcgggcag aagctggccc agcgcttccc cagcctgagc ctgtccgcgt
     8881 ccttcggctg gcaggcctac agcaccgccg ccctgggcgg ggccgccaac gcggcccgcg
     8941 cgctgggcgc caccctggcc accaccctgt tcgacggcgg ccgcctgcgc agccaggtgg
     9001 acgtgcagag cgcggtgcag gaacaggcgc tggtggccta ccagagcacc gtattgacgg
     9061 ccctggagga ggtggagaac gccctcaaga gccacgccgc ggcgcggcaa cgcttcgccg
     9121 cgcggcaggc ggcggccgag gcggcacgta atgcggccga cctgtcgcgc aagctctatc
     9181 aagcggggct ggcggacttc cagcaggtgc tggagaccca gcgcacccag ttgtcggcgg
     9241 aagacagcct ggccaccgcc gacgccagcc tgcgcaccag cctcatcagc ctgtacaagg
     9301 ccctcggcgg cggatgggat gccgccgccc ggaacggaac atcatgaacg acacctcttc
     9361 ctccgacagt ctcaagcagt tgctcgacca ggcgccccgc ggtgggcgcg gcgtggcgtg
     9421 gcgacgctgg ctttgggccg gcggcctcgt cctcgtgctg ggcggcgtct ttgtggccct
     9481 gcgcggcgac aagggcgcgg gcgggcatta cctcaccgag gccgccgagc ggggcgcact
     9541 ggtggtgacg gtgtccgcca ccggcaccct gcagcccacc acctcggtgg acgtgggcag
     9601 cgagcagtcc ggcaccctcg ccgaagtgct cgttcaggaa aacgaccggg tgaagcaggg
     9661 ccagctgctg gcgcgcctcg atctggccaa gctccaggac gcggtgagca agtcgcgggc
     9721 ggcgctggcg gcggcggaag cctcggtggc ccaggcgcgg gcgacggtgg cggaatcccg
     9781 cgcttcgctg gcgcgcatgc gccaggtggc cgagctctcc ggcggcaagg tgccgtccaa
     9841 gaccgaactg gagaccgccg aagccaccct ggagcgggcc gtggccaacg aggccagcgc
     9901 gcgggccacg gtgatgcagg cccaggctgc cctcaagacc gacgagacca atctgtccaa
     9961 gggcaccatc cgttcgccgg tgaatggcgt ggtcctgtcc cgcaaggtgg agcccggcca
    10021 gagcgtggtg gcggccatga ccatccccgt gctgttcacc ctcgccgaag acctgaccgc
    10081 catggagctc caggtcaagg tggacgaggc cgacgtgagc agcgtgcgcc tgggccagcc
    10141 ggccagcttt acggtggccg cgtggccggc gcggaccttc ccggcgacca tcgaacgggt
    10201 cggcatcggc tccaccatca ccgacaacgt ggtcacctac aagactgtgc tggccgtcaa
    10261 gaacgacgac ctggccctgc gtccgggcat gacggccacc gcctccatcg tcaccgccaa
    10321 acgggacgac gccctgctgg tgcccaacgc ggccctgcgc ttcacgcccc cggcccgcgg
    10381 tgcggacgcc cgccagggca gcttcgtctc cagcctgctg ccgcgcccgc cctccgagac
    10441 caagcacgcc accgcagccc ccgccggcag tccccaggtg tgggtgctgg gcgacgacgg
    10501 cccgcgggcc gttccggtga agaccggcgt cagcaacggg cgccagaccg agatcctggg
    10561 cggcgagctg aaacccggca tggcggtcat caccgaatac caggaaggcc gcccatgagc
    10621 gccgagcgcg ccgagcccct catccggctc gtcggcgtca ccaagaccta cggcgagggc
    10681 ggcgtggcct accaggcttt gcgcggcatc gacctggagg tggccgccgg cgagttcgtg
    10741 gccatcatgg gccccagcgg atcgggcaaa tccacggtga tgaacatcct cggctgcctg
    10801 gatacgccca caagcggcgc ctatctcttc cgcggcatcc acgtggagca actggaccgc
    10861 aaccagcgcg ccctgctgcg ccgccacagc ctgggcttcg tgttccaggg cttcaatctg
    10921 ctggcgcgca cctccgccca ggagaacgtg gagctgcccc tgctgtaccg gggcgaaacg
    10981 gcgccggcgc ggcacgccgc ggcccgcgcg gccctcgacc gcgtcggcct ggcgggctgg
    11041 gaaccccaca ccccggccga actctccggc ggacaacagc agcgcgtcgc cattgcgcgg
    11101 gccatcgtca cccagccgga cgtgctgctg gccgacgaac ccaccggcaa cctggacagc
    11161 cagcgcagcc acgagatcat ggcgctcctc gtcgaactca accgcaagca gggcatcacc
    11221 gtgctgatgg tgacccacga gcccgacatg gcggcctacg cccggcgcat cgtcaccttt
    11281 gtcgatggcc gcgtggcgcg ggacgaagcc gttccggagc gggccctatg agcagcgtgc
    11341 tggtcagcgc cttcctcctc gccctgcggg agatccgccg caacctgatg cgctccttcc
    11401 tcaccgtgct gggcatcgtc atcggcgtgg cggcggtggt gaccatggtc accctcggca
    11461 acggcgccac gcgggccgtg tcggaccaga tctccagcct cggcagcaac ctcttgatgg
    11521 tccgtcccgg ccagcgcctc ggccccggcc gcgacagcgc gggcgcgccg gccttcaaac
    11581 gggccgacgc cgaagccctg cgcacccagc tcaccggcat ggacgaagtg gtgccggtgg
    11641 cgggcagctc cgtcaccgtg gtggccggca acaacaactg gtccaccacc gtgagcggca
    11701 ccaccaacgg ctacctcaag gccggcaact ggaagatcgc cagcggccgc gcctttcagg
    11761 acgacgagga gcgcgccggc aagtccgtgt gcgtcatcgg cgccaccgtg cggcgcgaac
    11821 tctttggcac ccgcaacccc ctgggcgacg agatccgcgt caagaccttc gcctgccagg
    11881 tggtgggcct gctggccgcc aaagggcagg gcgccatggg catggaccag gacgacaccg
    11941 tgctgatgcc cctcgccacc gtgcagcgcc gcctcaccgg cacccaggac gtgtccacat
    12001 tgatggtgtc cctgcacgac ggcgtggacg ccaaacccgt catggcccag atccgcgccc
    12061 tgctgcggga acgccgcaag ctggccgaca acgaggccga caacttcaac gtgatggaca
    12121 ccaaacagat cgccgagacc ctctccggca ccatccgcac catgaccggc ctgctcggcg
    12181 ccgtggcggc ggtgagcctg ctggtgggcg gcatcggcat catgaacatc atgctcgtgt
    12241 ccgtcaccga acggacgcgg gagatcggca tccgcctcgc catcggcgcc ctggaacacg
    12301 aggtgctgct gcagttcctc atcgaagccg tggtgctctc cgcctgcggc gggctcatcg
    12361 gcatcctcct cgccctgctc gcctgcgtcg ccctctccgc cctgctgagc atgcccttcc
    12421 tcttcaacac cggcatcaac ctgctggcct tcctcttctc cgccctcatc ggcgtcgtgt
    12481 tcggctacat gcccgccc
//
LOCUS       DAOQPA010000103        12423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2609_length_12423_cov_19.147073, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000103 DAOQPA010000000
VERSION     DAOQPA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_12423_cov_19.147073"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /gene="rpoD"
                     /locus_tag="PKX01_13215"
     CDS             complement(<1..1503)
                     /gene="rpoD"
                     /locus_tag="PKX01_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HMZ77136.1"
                     /translation="MTTEKPKTPRKTPAKPRAAKVKDKLAPMIAEAPSAEEAEVRRSR
                     LKSLIALGKERGYLTYAEINDHLPDDLVDAEQIESIISTFNDMGIQVFDVAPAAEDLL
                     MSEVAPTVVDDEQAEAEAEQTLSAVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIA
                     KRIEEGLKHMIQAISACPTTIADILESADRISKDEMRIDELIDGLIDPNAVEDIEAMS
                     DSDELDVDEDAEDEDEDGDDGAGGAAVSASLIKLKADALERFDVLRGIYTKMIAALQK
                     KGPQDKTYVKLQEQISTELMNIRFTARTTERLCDAVRHMVEQARSHERKILHLCVDKA
                     HMPRTHFIKSFPGNETNLDWLKVEIGTNPSNAAVLMRMLPAVVEEQQKLVELQTRIGI
                     PLKDLKDINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
                     LMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK"
     gene            complement(1554..3413)
                     /gene="dnaG"
                     /locus_tag="PKX01_13220"
     CDS             complement(1554..3413)
                     /gene="dnaG"
                     /locus_tag="PKX01_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ77137.1"
                     /translation="MIPHAFVQDLLARVDIVEVIERYLPLKKGGVNYFACCPFHGEKS
                     PSFSVSPSKQFYHCFGCGAHGSAIGFLMEYSGLGFVEAVKDLAEQVGMQVPEEAPGAA
                     QERVREQSLTELMTQAARFYKEQLKRSPVAIEYLKGRGLTGEIAARYGLGYAPEGWQS
                     LQAAFADYADPKLLECGLVIENDQGRRYDRFRERITFPILDQRGHVIGFGGRVLGKGE
                     PKYLNSPETPLFEKGRELYGLVQARGTIRDSGTVLVVEGYMDVVALAQFGVANAVATL
                     GTAATPHHMQKLLRVADRVVFSFDGDAAGRRAAGRALEVCLEHLADNKTVAFLFLPDD
                     HDPDSYVRAFGADAFRQVVDHATPLADFLLAQLRQDIDLATAEGRARLVHEAKPLVTR
                     IGAPLLRLQVIKMLAEAAGFSQNEVEQACGLAAPRRSERRFDDTPPDDGFAARRFSGQ
                     ASSGKPFRGERRPAVPRQPPRQRPATPVETLLKIVVQHPTWSARLPVDLLPADSAEGL
                     ALIAVTDAMSVGDLPTQGVGALLEHFRDTPHAPILARVAVANEETPFEDGTIEPLLND
                     TLNKLQADALSREIERLTAQDRAGGLDPQQRRRLAELLMDKQRLRVRPKVTDS"
     gene            complement(3454..3900)
                     /locus_tag="PKX01_13225"
     CDS             complement(3454..3900)
                     /locus_tag="PKX01_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMZ77138.1"
                     /translation="MSLKDRINEDMKNAMRAKDAPRLSALRLLLAAIKQRQVDERIEL
                     DDDGVIAVVDKMLKQRRDSVAQYEAAQRFDLASAERFEIDVLTAYKPAGLGADEIAAA
                     IEAAVAATGAAGPADMGKLMAVLKPQLAGKADMAEVSKLVKTRLAN"
     gene            complement(4168..4380)
                     /gene="rpsU"
                     /locus_tag="PKX01_13230"
     CDS             complement(4168..4380)
                     /gene="rpsU"
                     /locus_tag="PKX01_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMZ77139.1"
                     /translation="MPGIRVKENEPFEVAIRRFKRTIEKVGLLPELRAREFYEKPTAE
                     RKRKLAAAVKRHHKRLRSQTLPPKMY"
     gene            4519..5544
                     /gene="tsaD"
                     /locus_tag="PKX01_13235"
     CDS             4519..5544
                     /gene="tsaD"
                     /locus_tag="PKX01_13235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ77140.1"
                     /translation="MRVLGIETSCDETGVALYETGRGLLGHCLHSQIDLHAVYGGVVP
                     ELASRDHIRRLPLLLRELLAKTGTTLADVDAVAYTSGPGLAGALLVGSAFAESLALAL
                     DLPVLPVHHLEGHLLSPLLSATPPAFPFVALLVSGGHTQLMRVTGVGDYHLLGETVDD
                     AAGEAFDKTAKLLGLGYPGGPALATLAQAGRPGRFRLPRPMLHSKDFDFSFSGLKTAV
                     LNAASAPDFTDADKADLAAEFQEAVVDVLVAKSLAALRHTGLRQLVVAGGVGANRRLR
                     DQLDGAAARRKATVFYPELALCTDNGAMIAFAGALRFAAGAPGRTGDGAFAVRPRWSL
                     QEIGTPV"
     gene            complement(5575..6183)
                     /gene="plsY"
                     /locus_tag="PKX01_13240"
     CDS             complement(5575..6183)
                     /gene="plsY"
                     /locus_tag="PKX01_13240"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HMZ77141.1"
                     /translation="MQTLLLLCAAYLIGSVPFAVVVSRAFGMADPRTYGSGNPGATNV
                     LRSGNKLAAALTLLGDAFKGWLAVFLAHLLVPTLRLGPEVVPACALAAFLGHLFPVFL
                     GFRGGKGVATAAGVLIGASGWLGLATLGVWLGVAFGARYSSLAALCAALAAPGLGFLL
                     GLDPYWILAISVMSGLLIWRHSDNIRRLLAGEESRIGSKKKA"
     gene            6235..6630
                     /locus_tag="PKX01_13245"
     CDS             6235..6630
                     /locus_tag="PKX01_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMZ77142.1"
                     /translation="MDFIFIEALRVQARVGIYPRERVAEQTLELNLTFGVPDAAAEHD
                     DIADTIDYAQVIERLRAELAVRQFNLIETLGEFVVKLLFDEFGAPWVKLAIAKMGVMK
                     GVKRVGVFIQRGRNGSAPPEHAPTAGLPG"
     gene            complement(6714..7100)
                     /locus_tag="PKX01_13250"
     CDS             complement(6714..7100)
                     /locus_tag="PKX01_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HMZ77143.1"
                     /translation="MDISGLSLTELRRLQTRIEAEIRRRSDTARKELLKRMQKLAAEH
                     GMSLADVLGQESEKPAAPARKTPPKALAQPARKAAKPASVIKFRHPENTAIGWTGHGR
                     KPQWVIDWLAQGKALEDLRASTEAAA"
     gene            complement(7233..7724)
                     /gene="ybaK"
                     /locus_tag="PKX01_13255"
     CDS             complement(7233..7724)
                     /gene="ybaK"
                     /locus_tag="PKX01_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HMZ77144.1"
                     /translation="MSRHDPHPPETPATRFLRQQHIAHSSHLYAYEDHGGTAVSAREL
                     NVPEHAVIKTLVMENERREALIVLMHGDRKVSTKELARQTGHKQIAPCDPAVAQRHTG
                     YMVGGTSPFGTRKALPVFMEQTILELPLIYINGGKRGFLVGIHPHDIIRTLHAKLVHV
                     AIE"
     gene            complement(7888..8379)
                     /locus_tag="PKX01_13260"
     CDS             complement(7888..8379)
                     /locus_tag="PKX01_13260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMZ77145.1"
                     /translation="MEPIFSAIAPAPFGAVGIATAGGCVTEIVFLPPGTAGLAPADPL
                     ADRAFRQIERYFADPDTAFSLPLRPQGSQFRQRVWQAIATIPRGETRCYGELARALGS
                     AARAVGQACGDNPFPLVIPCHRVIAAAGPGGFAHHRGGFLLDTKRWLLAHEAPGPFAE
                     CRD"
     gene            complement(8398..8985)
                     /locus_tag="PKX01_13265"
     CDS             complement(8398..8985)
                     /locus_tag="PKX01_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HMZ77146.1"
                     /translation="MPTPFRHALAALLLSTALGTPSTFAADVAGVHFDDHAAVAGSDT
                     VLNGAGLRTRFMLKVYAIGLYGPKRADTVETFAAVPGPKRIQIVTLRELTAEQFADAL
                     LDGIRKNTSDAEFTRLQPRADEFRAQLLALKSTPSGSQIRIEWLPASGTRLSVGNEVR
                     GRDIPGEDFFRALLRIWLGEKPVDGELRNALLGKA"
     gene            complement(9106..10383)
                     /locus_tag="PKX01_13270"
     CDS             complement(9106..10383)
                     /locus_tag="PKX01_13270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HMZ77147.1"
                     /translation="MDIKTYMQGVGRQARAASRQVAAASTDAKNTALLAMAAEIRARR
                     AELAAANRRDLEEARAAGLEPAMIDRLTLTEKGIDGMAAGLEQVAALPDPVGEMNELK
                     RRPSGITVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGKEALHSNQAI
                     AACVRAGLVAASLPEAAIQVVETTDRAAVGELITMPEFVDVIVPRGGKGLIERITREA
                     RVPVIKHLDGNCHVYVDAFADPAKALAIVENSKTQRYGTCNTTESLVVDESVAATQLP
                     ALGRMLAAKGVELRACPVALPILRDAGIPDVQLKAAVESDWYEEFLAPIIAVKVVDGL
                     DAAIDFINTCSSQHTESIVTENHTRAMRFLREVDSASVMINASTRFADGFEYGLGAEI
                     GISTDKIHARGPVGLEGLTSQKWIVFGDGHVRA"
     gene            complement(10394..11425)
                     /gene="holA"
                     /locus_tag="PKX01_13275"
     CDS             complement(10394..11425)
                     /gene="holA"
                     /locus_tag="PKX01_13275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMZ77148.1"
                     /translation="MILRPEQLAAQLDKPLAPLYVLHGNEPLLVLEAGDALRAAARRQ
                     GYSEREVLVTGQGFRWDSLQSAAGNLSLFGASKLVDLRIPNGKPGRDGGEALQRYARS
                     LDAGVVTLVTLPELDWAARKAAWFTALAEAGVVVELNAPERERLPEWIAQRLARQQQK
                     AQPEALAFIADHVEGNLLAAHQEILKLGLLHPPGELGLDAIRDAVLNVARYNVDALRA
                     ALLDGDAARCARLLEGLRGEGEAPPLVLWALASEVRTLAGLRQGVDSGQPLAALLKAE
                     RVFDERRRQGLQRALPRLGGGALNAALLHAARIDRMIKGLAGGDLWDECLQLCLRLCR
                     SPAPARTAN"
     gene            complement(11422..11949)
                     /gene="lptE"
                     /locus_tag="PKX01_13280"
     CDS             complement(11422..11949)
                     /gene="lptE"
                     /locus_tag="PKX01_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HMZ77149.1"
                     /translation="MSPLPRSAARRRLLGALLAGPLLASGCGFQLRGPRALPFESIYL
                     GMSEYSELAAAVRRQIRAGGPTRIADRPDDAQVRLEVLSDAREKVILALNTQGRVREY
                     QLRQRFAFRLVTKDGREVIAPNEITLRRDLAFDDSQLLAKEQEEILLYRDMQNDLVQQ
                     LMRRLAAAKMTPAQP"
     gene            complement(11946..12155)
                     /locus_tag="PKX01_13285"
     CDS             complement(11946..12155)
                     /locus_tag="PKX01_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77150.1"
                     /translation="MRPVACLIVSLLATTILGACSTQGLYEGIRQENVRRQNAPGRDV
                     SPDPAAPSYDAYNKERERVLKESRP"
     gene            complement(12172..>12423)
                     /locus_tag="PKX01_13290"
     CDS             complement(12172..>12423)
                     /locus_tag="PKX01_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77151.1"
                     /translation="GYGDDILAAAWPEPLDAALAQDAVDLMLQVNGKLRGAVRVAADA
                     PKDVIEAAALAHEATVKFLEGRAPKKVVVVPGRLVNVVG"
BASE COUNT         1858 a         3692 c         4688 g         2185 t
ORIGIN      
        1 cttgttgatg gtctcgatca tgtgcaccgg gatgcggatg gtgcgcgcct ggtcggcgat
       61 ggagcgcgtg atggcctggc ggatccacca ggtggcgtag gtcgagaact tgtagccgcg
      121 gcggtattcg aacttgtcca cggccttcat gaggccgatg ttgccttcct ggatcaggtc
      181 gaggaactgc aggccgcggt tggtgtactt cttggcgatg gagatgacca gacgcaggtt
      241 ggcttcggtc atttcgcgct tggcgcggcg ggccttggct tcgccggtgg acatctgctt
      301 gttgatgtcc ttgaggtcct tgagcgggat gccgatgcgg gtctgcagtt cgacgagctt
      361 ttgctgctcc tcgacgacgg cgggcagcat gcgcatgagc acggcggcgt tggacgggtt
      421 ggtgccgatc tcgaccttga gccagtccag gttggtctcg ttgccgggga agctcttgat
      481 gaagtgggtg cgcggcatgt gggccttgtc cacgcagagg tgcaggatct tgcgctcgtg
      541 gctgcgggcc tgctcgacca tgtggcgcac ggcgtcgcac aggcgctcgg tggtgcgcgc
      601 agtgaagcgg atgttcatca gctcggtgga gatctgctcc tggagcttga cgtaggtctt
      661 gtcctggggg cccttcttct ggagggcggc gatcatcttg gtgtagatgc cgcgcagcac
      721 gtcgaagcgt tccagggcgt cggccttgag cttgatgagg gaggcggaca cggcggcacc
      781 gccggcaccg tcgtcgccgt cttcgtcctc gtcctcggcg tcttcgtcca cgtccagctc
      841 gtcgctgtcg gacatggctt cgatgtcctc aaccgcgttg gggtcgatga ggccgtcgat
      901 gagctcgtcg atgcgcatct catccttgct gatgcggtcg gcggattcga ggatgtcggc
      961 gatggtggtg ggacaggcgg agatcgcctg gatcatgtgc ttgaggcctt cctcgatgcg
     1021 cttggcaatc tcgatttcgc cttcgcgggt gaggagctcc acggtgccca tctcgcgcat
     1081 gtacatgcgc acggggtcgg tggtgcggcc aaattcggaa tcgaccgcgg agagggtctg
     1141 ctcggcttcg gcttcggcct gctcgtcgtc caccacggtg ggggcgactt cggacatgag
     1201 gaggtcttcg gcggcggggg ccacgtcgaa gacctggatg cccatgtcgt tgaaggtgct
     1261 gatgatggac tcgatctgct cggcatcgac aaggtcgtcg ggcaggtggt cgttgatttc
     1321 ggcgtaggtg aggtagccac gttccttgcc gagggcgatc aggctcttga ggcgcgagcg
     1381 acggacttcc gcctcttcag cggaaggcgc ctcggcgatc atcggggcga gtttgtcctt
     1441 gaccttggcg gctcgcggct tggccggcgt cttgcgcggg gtcttcggtt tttcagtggt
     1501 catgactgtc ctcgggtact cggcaggcga ttttcgcgaa accgaaaatt atattacgaa
     1561 tccgtcactt tggggcgaac gcgtagcctc tgtttgtcca tcagcagctc ggcgaggcgg
     1621 cggcgctgct gcgggtcgag gccgccggcc cggtcctggg cggtcaggcg ctcgatttcg
     1681 cggctcaggg cgtcggcctg cagcttgttg agtgtgtcgt tgagcagggg ttcaatcgtc
     1741 ccgtcctcga acggggtttc ctcattggcg accgcgacgc gggccaggat cggcgcgtgg
     1801 ggggtgtcgc ggaagtgctc cagcagggcg cctacgccct gggtggggag gtcgccgacg
     1861 ctcatggcgt cggtgacggc gatcagggcg aggccctcgg cgctgtcggc gggcagcagg
     1921 tcaacgggca ggcgcgcgct ccatgtgggg tgctggacga cgatcttgag cagggtctcg
     1981 acgggcgtgg cagggcgctg gcggggtggc tgacgcggga cagcgggccg gcgttcgccg
     2041 cggaagggct tgccggacga cgcctggccg gaaaagcggc gagcagcgaa gccgtcgtcc
     2101 ggcggggtgt cgtcgaagcg ccgttcgctg cggcgtggcg cggcgaggcc acaggcctgc
     2161 tccacttcgt tctggctgaa accagccgct tcggcgagca tcttgatcac ctgcaggcgc
     2221 agcagcggcg cgccgatgcg ggtgacgagg ggcttggctt catggaccag gcgggcgcgc
     2281 ccctcggcgg tggcgaggtc gatgtcctgg cgcagttggg cgaggaggaa atcggccagc
     2341 ggggtggcgt ggtccacgac ctggcggaag gcgtcggccc cgaaggcgcg cacgtagctg
     2401 tccgggtcgt gatcgtccgg caggaacagg aaggcgacgg tcttgttgtc ggccaggtgt
     2461 tcgaggcaca cctccagcgc ccggccggcg gcgcggcggc cggcggcgtc accgtcgaag
     2521 gagaacacca cccggtcggc gacgcgcagg agcttctgca tgtggtgggg cgtggcggcg
     2581 gtgcccaggg tggcaaccgc gttggcgacg ccgaactggg cgagggcgac cacgtccatg
     2641 tagccttcga cgaccagcac ggtgccgctg tcacggatgg tgccgcgggc ctggacgagg
     2701 ccgtagagct cgcgcccttt ctcgaacagc ggtgtttcgg gcgagttgag gtacttgggc
     2761 tcacccttgc ccaggacccg gccgccgaag ccgatgacgt ggccgcgctg gtcgaggatg
     2821 gggaacgtga tgcgttcgcg gaagcggtcg tagcggcggc cctggtcgtt ctcgatgacg
     2881 agcccgcatt cgagcagctt ggggtcggcg tagtcggcga aggccgcctg gagactctgc
     2941 cagccctcgg gcgcgtagcc caggccgtag cgggcggcga tctcgccggt gaggccgcgg
     3001 cccttgaggt attcgatggc gaccgggctg cgcttgagct gctccttgta gaagcgcgcg
     3061 gcctgggtca tcagctcggt gagggactgt tcgcggacgc gttcctgggc ggcgccgggg
     3121 gcttcttcgg gcacctgcat gccgacctgc tcggccaggt ccttgacggc ctcaacgaag
     3181 ccgaggccgc tgtactccat caggaagccg atggcgctgc cgtgggcgcc gcagccgaag
     3241 cagtggtaga actgcttgga cgggctgacg gagaaggaag gggatttttc cccgtggaag
     3301 gggcagcagg cgaagtagtt gacgccgccc ttcttgaggg gcaggtagcg ctcgatgact
     3361 tcgacgatgt cgacccgcgc gagcaggtcc tggacaaagg cgtgcgggat catgtggcaa
     3421 cggccggcgc aggcggcgcc gttcccatga atcctagttc gcgagtcggg ttttcaccag
     3481 tttcgacacc tcggccatgt cggccttgcc ggcgagctgc ggcttgagca cggccatcag
     3541 cttgcccatg tcggcggggc cggccgcacc ggtggcggca acggcggcct cgatggccgc
     3601 ggcgatctcg tccgcgccca ggccggccgg cttgtaggcg gtgaggacgt cgatctcgaa
     3661 gcgctcggca ctggccaggt caaagcgctg ggcggcctcg tactgggcga cggagtcgcg
     3721 gcgctgcttg agcatcttgt ccaccacggc gatcacgccg tcgtcgtcca gctcgatccg
     3781 ctcatccacc tggcgctgct tgatggcggc cagcagcagg cgcagcgcgg acaggcgcgg
     3841 agcgtccttg gcgcgcatgg cgttcttcat gtcttcgttg atgcgatcct tgagggacat
     3901 gttcgggctc cagggcgagg gtgggcaaag gcgccgtcac cggacgagcg gtgaggtcgc
     3961 gacggaacgg acgacggaca ggcctgggca gcgctcgacg tcacccggca gcgccatcga
     4021 cagtcgatgg cgcggtgatg ttgcggcgta gtgttggagt gctcgcgctg aactttgcga
     4081 cgggcatgcc gcaaagcacg aacgccacgt cgctacgcac acaggtgcgt cagcgcgtgg
     4141 cgatcgggaa gcccgtacag gtgccgctca gtacatcttg ggcggcaggg tctggctgcg
     4201 caggcgcttg tggtgacgct tgacggcggc ggcgagcttg cgcttgcgtt cggcagtggg
     4261 cttttcgtaa aactcgcggg cgcggagttc aggcagcaga cccacttttt cgatggtgcg
     4321 cttgaagcgg cggatggcaa cttcgaacgg ttcgttttct ttgacgcgga tacccggcat
     4381 tgcgtgcttc cttgaaaatc tggattctgt gagcgggaaa accatggagt ataaccaagg
     4441 cccgcttgac aaccaagccc ttttttcgac gcgcgtgacc ggccgcgcta aaataccggc
     4501 ttttgcacgg acgggaccat gcgagttctg ggcatcgaaa cctcctgtga cgagaccggc
     4561 gtcgccctct acgagacggg ccggggcctg ctcgggcact gcctgcattc ccagatcgac
     4621 ctgcacgcgg tgtatggcgg cgtggtgccg gagctggcgt cccgcgatca catccgccgc
     4681 ctgccgctgc tgctgaggga actgctggca aagacaggca cgacgctggc cgacgtggat
     4741 gcggtggcct acacctccgg tccgggcctt gccggcgcgc tgctggtggg ctcggcattt
     4801 gccgaatctc tggcgctggc gctcgacctg ccggtgctgc cggtgcatca cctggaaggg
     4861 catctgctgt cgccgctcct gtcggcgacg ccaccggcct tccccttcgt ggccctgctg
     4921 gtgtcgggtg gccacaccca gctgatgcgc gtcaccggcg tgggcgatta ccacctcctc
     4981 ggcgagaccg tggacgacgc ggccggcgag gccttcgaca agaccgccaa gctgctcggc
     5041 ctgggctatc ccggcggccc ggcgctggcg acgctggccc aggcggggcg gcccggacgt
     5101 ttccgcctgc cgcggccgat gctccacagc aaggacttcg acttcagctt cagcgggctc
     5161 aagacggccg tactcaatgc ggcgtcggca ccggacttca ccgacgcgga caaggcagat
     5221 ctggcggcgg agttccagga ggcggtcgtg gacgtgctgg tggccaagtc cctcgcggcc
     5281 ctcaggcaca cggggctgcg ccagctggtg gtggcgggcg gcgtcggtgc caatcgccgc
     5341 ttgcgtgacc agctcgatgg ggcggcggcg cggcgcaagg cgacggtgtt ctaccctgag
     5401 ctggcgctgt gcaccgacaa cggcgcgatg atcgccttcg ccggcgcgct gcgctttgcc
     5461 gccggtgcgc cggggcggac cggggacggc gcgtttgcgg tgcgcccgcg ctggagcctg
     5521 caggaaatcg gcaccccggt gtgagaggcg tcgccggttc ggcggcgcgg tgcgctatgc
     5581 cttcttcttg ctgccgatgc ggctttcctc gcctgccagc agacgccgga tgttgtcgct
     5641 gtggcgccag atcagcagcc ccgacatgac gctgatagcc agaatccagt agggatcgag
     5701 accgagcagg aagcccaggc ccggagccgc cagcgcggcg cagagggcgg ccagcgacga
     5761 atagcgggcg ccgaaggcga cgcccagcca aacgccgagc gtcgccagac ccagccagcc
     5821 ggacgcaccg atcaacacgc cggcggcagt ggccacgcct ttaccgcccc gaaagcccag
     5881 aaatactggg aaaaggtgcc ccagaaaggc ggccagtgcg caggccggaa cgacttcggg
     5941 tccgagtcga agcgtgggca cgagcaggtg ggccaggaac accgccagcc aacccttgaa
     6001 cgcgtcgccg agcagggtga gggcggctgc gagcttgtta ccgctgcgca ggacgttggt
     6061 ggcgccggga ttgcccgaac cgtatgtgcg ggggtcggcc atgccgaagg cgcggctgac
     6121 cactaccgca aaggggacgg agccgatcag gtaggcggcg cagaggagga gcagggtttg
     6181 catgcggttc cggtgggcgg gctagaatgg cggcgatttt agccgggaca tgcgatggat
     6241 ttcattttta tcgaagcact gcgggtgcag gcgcgggtgg gcatctatcc ccgcgagcgg
     6301 gtcgcggagc agaccctcga gctcaatctg accttcggag tgcccgatgc ggccgccgag
     6361 cacgatgata tcgccgacac catcgattac gcccaggtca tcgagcgatt gcgggcggag
     6421 ctggccgtgc gtcagttcaa tctgatagaa accctgggtg aattcgtcgt caaattgctt
     6481 tttgatgaat ttggtgcgcc ctgggtgaaa ttggcaattg ccaaaatggg cgtgatgaaa
     6541 ggtgtgaaac gggtgggtgt attcattcag cggggccgta acggaagtgc gccaccggaa
     6601 catgcaccga ccgcgggttt gcccgggtga atagaactgc cgcaagaaaa aggagtgccc
     6661 ggaccggcac tccttttttc gttgcattga cgcataaggc gtgacgaaag cgctcaggcc
     6721 gcggcttcgg tggaggcgcg caggtcttcg agtgccttgc cctgggccag ccagtcgatg
     6781 acccactggg gcttgcggcc gtgcccggtc cagccgatgg cggtgttttc gggatggcgg
     6841 aacttgatca ccgaggccgg tttggccgcc ttgcgggcgg gctgtgcaag cgccttggga
     6901 ggcgtcttcc tggcaggcgc ggcgggcttt tcggactcct gcccgaggac gtcggcgaga
     6961 ctcatgccgt gctcggcagc cagtttctgc atgcgcttga ggagttcctt tcgggcggtg
     7021 tcagaacgtc ggcggatttc ggcttcgatg cgggtctgca gacggcgcaa ttcggtgagc
     7081 gagagtccgg aaatgtccat ggtgaaattc cttgtcagaa agtcgggtgc accgggggcg
     7141 cggcgctggc gcgggattgg ccggatgatc aattttgagt atgggcgaat tgtgcatcaa
     7201 ccgttgccaa ttgaatagcg tgaggccgat gttcattcga tggcaacgtg caccagcttt
     7261 gcgtggagcg tccggatgat atcgtggggg tgaatgccga ccaggaaacc ccgtttgccg
     7321 ccattgatat aaatgagcgg gagttcgagg atggtctgct ccatgaatac cggcagggcc
     7381 ttgcgggtac cgaaagggga ggtgccgccc accatgtagc cggtatggcg ctgggccacg
     7441 gccgggtcgc agggggcgat ctgcttatgc ccggtctggc gggcaagctc cttggtcgag
     7501 actttccggt ccccatgcat gagcacgatg agggcctccc gcctttcgtt ctccatgacg
     7561 agagtcttga tcaccgcgtg ctccgggaca ttcagctcgc gcgcggaaac ggcagtgccg
     7621 ccatgatcct cgtaggcata gaggtggctg gagtgggcaa tgtgctgctg gcgcaggaaa
     7681 cgggtggcag gcgtctccgg agggtgcggg tcgtgacggg acatgatggt gaatacaggc
     7741 tggaaaagga atgcgtcagt tgggctgggc ggacaggatg gcgcgggacg gagcgcgaag
     7801 tgatccatat gtacgcgatt ccgtgcgcac gggggcgccg ggactattgt gcgtccactt
     7861 cggcgccggc gctacggcag gccgcggtca atccctgcat tccgcaaaag gcccaggcgc
     7921 ttcatgtgcg agcagccagc gtttggtgtc gaggagaaag cctccgcggt gatgggcaaa
     7981 cccgccggga cctgcggccg cgatcacgcg gtgacaggga atgaccagcg gaaaggggtt
     8041 gtcgccgcag gcctgaccga cggcgcgggc cgcgctgccc agagcccggg ccaattcgcc
     8101 ataacagcgg gtttctccgc ggggaatggt ggcaatggcc tgccagacac gctggcggaa
     8161 ttgactgcct tgaggccgta atggaaggga aaaggcggta tccggatcgg cgaaatagcg
     8221 ttcgatctgc cgaaaagccc gatctgccag gggatcggcg ggcgcgaggc ccgccgtgcc
     8281 gggtggcagg aagacgattt cggtgacgca gccccccgca gttgcaatgc ccaccgcccc
     8341 gaacggggcc ggtgcgatgg cgctgaagat cggttccatc gtccggtgtg attgcgttca
     8401 ggctttgccg agcagggcgt tgcgcaattc gccatcgacc ggtttttcgc cgagccagat
     8461 gcgtagcagg gcgcggaaga aatcctcccc gggaatgtcg cggccgcgga cctcattgcc
     8521 gacgctgagg cgggtgccgc tggcaggcag ccattcaatg cggatctggc tgccggacgg
     8581 ggtggatttg agcgccagca attgggcgcg gaattcgtcg gcacggggct ggagtctggt
     8641 gaattccgcg tcgctggtat tttttcttat tccgtccagg agggcgtcgg cgaattgttc
     8701 ggcggtgagc tcccgcaggg tcacgatctg aatgcgtttt gggcccggca cagcggcgaa
     8761 ggtctcgacc gtgtcggcgc gtttgggccc gtacaggccg atggcgtaca ccttgagcat
     8821 gaatcgggta cgcaggccgg cgccattgag gacggtgtcg ctgccggcga cggcggcgtg
     8881 gtcgtcgaaa tggacgccgg cgacgtcggc ggcaaaggtg gagggcgtgc cgagcgcggt
     8941 gctcagcagc agcgcggcga gggcgtggcg gaacggggtg ggcatgggct gtctcctgga
     9001 ttcttggtat gggaaaacgc ccgccggatc gggcggaggc tcgattcgaa cgggcgtttg
     9061 aatcctagcc gagaagcccc cggcagggaa gggcgatgac gacgctcagg cgcgcacgtg
     9121 gccgtcgccg aagacgatcc acttctggct ggtgaggcct tccaggccca ccgggccgcg
     9181 ggcgtggatc ttgtcggtgg agatgccgat ctcggcaccc aggccgtatt cgaagccgtc
     9241 cgcgaagcgc gtcgacgcgt tgatcatcac cgacgcggaa tccacctccc gcaggaagcg
     9301 catggcccgc gtgtggtttt cggtgacgat ggactcggtg tgctgggacg agcaggtgtt
     9361 gatgaagtcg atggccgcat ccaggccgtc caccaccttg acggcgatga tgggggcgag
     9421 gaattcctcg taccagtccg actcgaccgc ggccttgagc tgcacatcgg gaatgccggc
     9481 gtcccgcagg atgggcaggg ccaccgggca ggcgcgcagc tccacgccct tggcggcgag
     9541 catgcgcccg agggcgggca gctgggtggc ggcgacgctc tcatccacca ccagggattc
     9601 ggtggtgttg caggtgccgt agcgctgggt cttggaattc tcgacgatgg ccagggcctt
     9661 ggcggggtcg gcaaaggcgt ccacatagac gtggcagttg ccgtccaggt gcttgatgac
     9721 ggggacgcgg gcctcgcggg tgatgcgctc gatgaggccc ttgccaccac gggggacgat
     9781 cacgtccacg aattcgggca tggtgatgag ttcgcccacg gcggcccggt cagtggtctc
     9841 gacaacctgg atggcggctt cgggcaggct ggcggcgacg agcccggcgc gcacgcaggc
     9901 ggcgatggcc tggttggagt gcaaggcttc cttgccgccg cgcaggatgg cggcattacc
     9961 ggacttgagg cacagggcgg cggcgtcggc ggtcacgttg gggcgggctt cgtagatgat
    10021 gccgatgacg cccagcggca cgcgcatctt gccgacggtg atgccggacg ggcggcgctt
    10081 gagctcgttc atctcgccca ccgggtcggg cagggctgcg acctgttcga ggccggcggc
    10141 catgccgtcg atgccttttt cggtgagggt caggcggtcg atcatggccg gctccaggcc
    10201 ggcggcgcgg gcttcttcca ggtcgcggcg gttggcggcg gcgagttcgg cccggcgggc
    10261 gcggatctcg gcagccatgg cgaggagggc ggtgttcttg gcgtcggtgg aggcggcggc
    10321 cacctggcgt gaggcggcgc gggcctgacg gccgacgccc tgcatgtagg tcttgatgtc
    10381 catcgtgtct cgttcagttc gcggtgcggg ccggggcggg gctgcggcac aggcgcaggc
    10441 acagttgcag gcattcgtcc cagaggtcgc cgccggccag acccttgatc atccggtcaa
    10501 tcctcgccgc gtgcaagagc gcggcgttca gcgcgccgcc gcccaggcgc ggaagggcgc
    10561 gctgcaggcc ctggcgccgg cgctcgtcga agacccgttc ggccttgagg agggccgcca
    10621 gcggctggcc ggaatcgacg ccctggcgca ggccggcgag ggtgcggact tcgctggcca
    10681 gggcccacag caccagggga ggcgcctcgc cttcgccgcg caggccttcg agcaggcgcg
    10741 cgcagcgggc ggcgtcgcca tccagcaggg cggcgcgcag ggcgtccacg ttgtagcggg
    10801 cgacgttgag caccgcgtcg cggatggcgt cgaggcccag ttcgcccggc gggtggagca
    10861 ggcccagctt gaggatttcc tggtgggcgg cgaggagatt gccttccacg tgatcagcga
    10921 tgaaggcgag ggcctcgggc tgcgccttct gctgttggcg ggcgaggcgc tgggcgatcc
    10981 actcgggcag gcgttcccgc tccggggcgt tgagttcgac gaccacgccg gcttcggcga
    11041 gggccgtgaa ccaggcggcc ttgcgggcgg cccagtccag ctccggcagg gtgacgaggg
    11101 tcacgacgcc ggcgtcgagg ctgcgggcgt agcgttgcag ggcttcgccg ccgtcgcggc
    11161 cgggcttgcc gttggggatg cgcagatcca ccagcttgct ggcaccgaac agggacaggt
    11221 tgccggcggc cgactgcagg ctgtcccagc ggaagccctg cccggtgacg agcacctccc
    11281 gctcggagta gccctgtcgg cgggcggcgg cgcggagggc gtcgccggct tcgagcacca
    11341 gcaggggctc gttaccgtgc aggacgtaaa ggggcgcgag gggcttgtcg agctgggcgg
    11401 cgagctgctc ggggcgcagg atcacggctg ggccggcgtc atcttggccg cggcgaggcg
    11461 gcgcatgagc tgctgcacca gatcgttctg catgtcccgg tagagcagga tctcttcctg
    11521 ctccttggcg aggagctggg agtcgtcgaa ggccaggtcg cggcgcaggg tgatctcgtt
    11581 gggggcgatc acctcccgcc cgtccttggt cacgagccgg aaggcgaagc gctggcggag
    11641 ctggtattcg cgcacccggc cctgggtgtt gagggccagg atgacctttt cgcgggcgtc
    11701 ggacaggact tccaggcgga cctgggcgtc gtcggggcgg tcggcgatgc gggtcgggcc
    11761 gcccgcgcgg atctggcggc ggacggctgc ggccagttcg gaatattcgc tcatgcccag
    11821 gtagatcgat tcgaagggca gggcgcgcgg accgcgcagc tggaagccgc aaccgctggc
    11881 cagcagcggc ccggccagca gggcgccgag gagtcgccgg cgggcggcgg agcggggaag
    11941 cgggctcatg ggcgggattc cttcaggacg cgctcgcgtt ccttgttgta ggcatcgtag
    12001 gacggcgccg ccgggtcggg gctgacatcg cggcccggcg cattctggcg tcgtacgttc
    12061 tcctgccgga taccctcgta gagaccctgg gtggagcacg cgcccaggat ggtggtggcg
    12121 agcaggctga cgatcaggca ggcgactggg cgcatggctt tctccggggg ctcagccaac
    12181 cacgttcacg aggcggccgg ggacgaccac gaccttcttc ggggcgcggc cttcgaggaa
    12241 cttgacggtg gcctcgtggg ccagggcggc ggcttcgatg acatccttgg gggcatcggc
    12301 ggccacgcgc acggcgccgc gcagcttgcc gttcacctgc agcatcaggt ccaccgcgtc
    12361 ctgggcgagg gcggcgtcaa ggggctcagg ccaggcggcg gcgaggatgt cgtcgccgta
    12421 acc
//
LOCUS       DAOQPA010000104        12289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2640_length_12289_cov_19.192251, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000104 DAOQPA010000000
VERSION     DAOQPA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12289
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_12289_cov_19.192251"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..581
                     /gene="metW"
                     /locus_tag="PKX01_13295"
     CDS             3..581
                     /gene="metW"
                     /locus_tag="PKX01_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HMZ77152.1"
                     /translation="MRYDYDVIANWIEPGERVLDLGCGDGSLLRHLGEVRRIRGYGVE
                     NDPERVLACARNGVNVIQIDLEEGLAGFENGFFDHVIMSLSLQAMHNTQRILQEMLRV
                     GREAVVSFPNFAYWRHRQAIINGHMPVSDSLPHQWYNTPNVRFFTIADFDDLCAREGI
                     AIHERLAFDDHKVVLEEPNFLASVALYRLGRA"
     gene            complement(658..1440)
                     /locus_tag="PKX01_13300"
     CDS             complement(658..1440)
                     /locus_tag="PKX01_13300"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ77153.1"
                     /translation="MQSFNSPRAVLTALLMSAGLGTAWAGPVVSTPFDLEISSTPFVT
                     GTAYAGVSVNTTASVSTPLDVNGRAWTDLQYVEDPLVWTGVRYNYPAPSSPQIATDPT
                     HCSSSRPGLCSYIWYDPNHTSSGTNGVTRAYFRTTFNLPEFTGDAAPLGAVLRVRADD
                     DFAVWINGFLVMLNADYGTSGDKGPDYVFERGVSQYLRQGELNVMTFVATDGSLNSPS
                     NVSYEHLAFELKLNNQVPEPASLMLGLMGLGGIGLARRRRTG"
     gene            1643..2497
                     /locus_tag="PKX01_13305"
     CDS             1643..2497
                     /locus_tag="PKX01_13305"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HMZ77154.1"
                     /translation="MNTPQAGILLPVPPLARYLGFTLEPDSDVHAGLSALLDVVDGER
                     TVAGLGLATVTAAGLSVPGLHAFPTPSAPGIELPATPAALWLWLRGEDRGELHLRAQR
                     IEALLAPVFRLVDSIDAFKHDTGRDLSGYEDGTENPQGDDATTAAIAADGSSLVAVQR
                     WLHDFRRLAAMAPEARDDCIGRRRSDNEELDDAPASAHVKRTAQESFSPEAFLLRRSM
                     PWSEGRDAGLMFVAFGATLDPFEAQLRRMTGQEDGIVDALFRFTRPLTGAYFWCPPVR
                     DGKLCLIR"
     gene            complement(2632..3375)
                     /locus_tag="PKX01_13310"
     CDS             complement(2632..3375)
                     /locus_tag="PKX01_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77155.1"
                     /translation="MTDILRAMRIVVASLLVSGLVGAANAGPYQTYPLSARTDYLWMA
                     TDNATLGENWIRGLDGNNPAAVDAQHPYGNSATTALAAEDMMWYCPPGGCASNTRTGL
                     AYFSTVFNLNSDFRVAGGQLKIIADDYFELYLNGTLITFGLLDMHMDASGQPVPIVLD
                     LSLYKDDFYGGENLIAIKAMDGYQMSPGGVCSGTTMQTQVGDFCQSARGFEYVYVSGA
                     IDTIPEPPVGSLMVFSMAALAAMRRFGRS"
     gene            complement(3578..5293)
                     /locus_tag="PKX01_13315"
     CDS             complement(3578..5293)
                     /locus_tag="PKX01_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77156.1"
                     /translation="MRTLAGRAPGGEPWAALFAEALSEADPDAAALVGAAAVDGIVAD
                     GRACAQLMEWEARLAALATRPCGGPSAQAAAALALRRGLVALVLRGDAQAAWTIWDDG
                     VHQAERAASEPLQLALTGARGVAECLAGAVTRADVLVSDAWVLCEVKARSRLPCLLVT
                     VALSLIHLMLDRSSDALALLQRTCAAAAFEGLPESLQVLVWSLGMHAAADVGDTDRVA
                     DWSRAVADRTVPVPRLLLHACRHYVLGAMALRQGDALRARIHADACIGVGERAGALLP
                     GYIGRLLRLQALADLADAGASLRTEAAQWIPTWSACGMARLAAVAHLELAARDVRAGE
                     LDRARRAWFAALAALPPGESLRPLNRSGVWLETLRVRLFPMGDRCDATTAAVRIRTLG
                     EFSVEIGGRRIFDRDWKGSRTKTLLMALICEGGSKVSVDRLADLLWPDSDGAQARQNL
                     KVALWRLRRLGCEGPPEVNWVHVKQGRVSLPAGLVQVDALEFRNVARAALMPGADVGL
                     MREALAAQAESFLPMEDNLPWVAAFRKGLMGLKQAVQARIAGCGEQGAVRVVGEACTS
                     ELNGA"
     gene            5590..6114
                     /locus_tag="PKX01_13320"
     CDS             5590..6114
                     /locus_tag="PKX01_13320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HMZ77157.1"
                     /translation="MMNSRQKPLSPPVVEGDAPLRPDARDLWQLWFDGAAHPNPGRLG
                     LGGVLIAPDGRRHAYSALAPECGCNNEAELLALGRGLELARGAGATHVQVWGDSDFAV
                     RHLNGAAHTDIPRLRALLDVIGRQLTGFTLVHLHWIPRHRNHDADRLSRAALGLPDKP
                     APRPVSARKRRGRR"
     gene            complement(6116..6646)
                     /locus_tag="PKX01_13325"
     CDS             complement(6116..6646)
                     /locus_tag="PKX01_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HMZ77158.1"
                     /translation="MTPSTTEIRLVPTADDHRVARHAAAAIVLTVAEAAIPLPLPGVK
                     PGLANIITLVVLARWGWRDAAWVAILRILAGSLLLGQFLAPGFFLSLAGGLASLAVLG
                     AAMHLPRRWFGPVTQSVLAAFAHIGGQVLVARLWLVPHDGVFYLVPVFALAATVFGLG
                     NGLIAARLLAEEVPGR"
     gene            complement(6627..7034)
                     /locus_tag="PKX01_13330"
     CDS             complement(6627..7034)
                     /locus_tag="PKX01_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HMZ77159.1"
                     /translation="MSRLADLRTWLAAWVAYLRVGDVLVLALGLGASAASALVLWQGG
                     APDKAVIKAGGRVFAEVDLSAPRIVEVPGPIGTTRIEIQPGRARVAADPGPRQYCVRQ
                     GWLHSAGAVAICAPNEVSLSLSGRNAAYDSLNY"
     gene            complement(7031..7873)
                     /gene="rapZ"
                     /locus_tag="PKX01_13335"
     CDS             complement(7031..7873)
                     /gene="rapZ"
                     /locus_tag="PKX01_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HMZ77160.1"
                     /translation="MQLVLISGLSGSGKSVALNVMEDSGHYVVDNLPSTLLPQLISNL
                     RGGGYQRVAVAVDVRSGASIAALPQQVALLRGMVTDLRFLFLEARDETLIARFSETRR
                     KHPLGDECSTLAEAIGREREALALISELGQRIDTSDISANTLRNWVKSFMDLEASSQI
                     TLLFQSFGFKHGIPVDADLVFDVRCLPNPHYNPELRPLTGLDAPVIDFLERVPEVGRM
                     VEDIYAFVASWLPAYVHDNRSYLTIAIGCTGGQHRSVYVAEQLAGRFRESARVLVRHR
                     SFRR"
     gene            complement(8029..8196)
                     /locus_tag="PKX01_13340"
     CDS             complement(8029..8196)
                     /locus_tag="PKX01_13340"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HMZ77161.1"
                     /translation="MDTSDENPCVGICEIDESGYCVGCGRSADEIYGDSEPAPCATDT
                     LPAVEAPPPDA"
     gene            8481..9080
                     /locus_tag="PKX01_13345"
     CDS             8481..9080
                     /locus_tag="PKX01_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ77162.1"
                     /translation="MKIKTLAAALLSLSALHAHATVIDFNQVIGFQTLPSPYQEDGFT
                     ITNSSTNSSAMLFWGTLSYNADPNGNTYSHNYGGTTSTLSKTGGGSFTFNSIDLTDVY
                     NFGSGGSVVFDFLYSNNSTSHSTVNLPTVAGLHTFTFDLANLTSVSWTPVGTQGNWIQ
                     VDNIVLDTTLSNRVPEPTSMALVGLGVAMAGALRRRRAA"
     gene            complement(9130..9357)
                     /locus_tag="PKX01_13350"
     CDS             complement(9130..9357)
                     /locus_tag="PKX01_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HMZ77163.1"
                     /translation="MSVASPCTGICRIDPMTGWCAGCRRTLTEIAEWGAAGDGRRQAI
                     LDGLAQRRWPEGAPADKASADVVPPPAASTP"
     gene            complement(9415..10338)
                     /locus_tag="PKX01_13355"
     CDS             complement(9415..10338)
                     /locus_tag="PKX01_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMZ77164.1"
                     /translation="MTLPARVRIVEVGPRDGLQNEAQVIATDTKVALIDRLARAGLPA
                     VEATSFVSPKWVPQMGDAAEVLARIERLPGVDYPVLTPNLKGFDAALAAGAKSVAVFA
                     AASESFSRRNINCSVAESLARFEPIMAAADEAGVRVRGYVSCVLGCPYEGAVAPSAVA
                     EVAGTLYDMGCYEVSLGDTIGTGTPGAARRMLDAVAQRVPVEQLAGHYHDTYGQALAN
                     VYASLQAGVAVFDASVGGLGGCPYAAGASGNLATEDLVYLLNGLGIASGVDLEALVDI
                     AAWISAQLGRPPASRVARASLAHRGRRGCAA"
     gene            complement(10350..>12289)
                     /locus_tag="PKX01_13360"
     CDS             complement(10350..>12289)
                     /locus_tag="PKX01_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HMZ77165.1"
                     /translation="ARRLGIRTVAVYSEADAGARHVRLADEAVLIGPAAARESYLVIE
                     RIIEAAQRTGAQAIHPGYGFLSENEAFCEACEAAGIVFIGPPVAAIRAMGSKSEAKKL
                     MEQAGVPLTPGYHGDDQTPATLQREADAIGYPVLIKAAAGGGGKGMRRVECSADFLDA
                     LASCKREAASSFGDDHVLVEKYILRPRHIEIQVFGDSHGHAVYLFERDCSVQRRHQKV
                     LEEAPAPGMTPERRAAMGRAAVEAARAVGYVGAGTVEFIAQQDGSFYFMEMNTRLQVE
                     HPVTEMITGLDLVEWQLRVAAGEPLPLAQEQLALNGHALEARIYAEDPGKGFLPATGR
                     LLHLAPPDEGLHVRVDTGVEEGDEITPHYDPMIAKLIVWDVNRDRALARMLQALADYR
                     VAGVANNIEFLSRLTACPAFANADLDTGLIEREQAYLFPEASAPERDEWLALALAELL
                     REQERAAATAARSADPSSPWHARDGWRMNAAPARSLIFRHGEAQKTLRVATLPGAFEI
                     TLDGVATTVSGSLNPRGLLRGEFGGRRLAATVVAAGERRHVFAHGRARVFACVDPLFF
                     SGEGGGAEGGLLAPMPGKVIALLAAEGARVDKGAPLLILEAMKMEHTIAAPTAGTVKG
                     FRFAVGDQVADGAELVEFDAA"
BASE COUNT         2057 a         4528 c         3884 g         1820 t
ORIGIN      
        1 gtctgcgcta cgactacgac gtgatcgcca actggatcga acccggcgag cgggttctcg
       61 acctgggctg cggcgacggc agcctgctgc gccacctggg cgaggtgcgc cgcatccgcg
      121 gctacggcgt cgagaacgac cccgaacggg tgctcgcctg cgcccgcaat ggtgtcaacg
      181 tgatccagat cgacctggaa gaaggcctcg ccggcttcga aaacggcttc ttcgaccacg
      241 tcatcatgag cctgtccctg caggccatgc acaacaccca gcgcatcctg caggagatgc
      301 tgcgggtcgg ccgcgaggcg gtggtgagct ttcccaactt tgcctactgg cgccaccgcc
      361 aggccatcat caacggccac atgccggtgt ccgacagcct gccgcaccag tggtacaaca
      421 cccccaacgt gcgcttcttc accatcgccg atttcgacga cctgtgcgcc cgcgagggca
      481 tcgccatcca cgaacggctg gccttcgacg accacaaggt ggtcctcgaa gaacccaact
      541 ttctcgccag cgtggccctt taccggctcg gtcgggcctg actgacaaag aaccggccca
      601 tttacgccac gacgccccgc aaacggggcg tcgtgtccgc aaggccggcc gccggcctca
      661 gccggtgcgg cggcgccgcg ccagaccgat cccgccgagg cccatcaggc ccagcatcag
      721 gctcgccggt tcgggcacct ggttgttcag cttcagctca aaggcgaggt gctcgtagct
      781 cacgttgctc gggctgttga gactgccgtc ggtggcgacg aaggtcatca cgttgagctc
      841 accctgccgc agatattggc tcacgccccg ttcgaacacg taatccggcc ccttgtcgcc
      901 cgacgtgccg taatcggcat tcagcatcac caggaagccg ttgatccaca cggcaaaatc
      961 atcatcggcg cggaccctca gcaccgcccc caggggcgcc gcatcgccgg tgaattccgg
     1021 caggttgaaa gtcgtgcgga aatacgcccg ggtgaccccg ttcgtcccgc tggacgtatg
     1081 gttcgggtca taccagatgt acgaacacag tccgggccgg ctcgacgaac aatgggtcgg
     1141 atcggtcgcg atctgcgggg acgacggggc cggatagttg tagcggaccc ctgtccacac
     1201 caacggatcc tccacgtact gcagatccgt ccacgcgcgg ccattcacgt cgaggggcgt
     1261 gctgacactc gccgtggtat tgacgctcac ccccgcgtag gccgtacccg taacgaaggg
     1321 cgtcgatgaa atctccagat cgaagggcgt cgacaccacc ggtcctgccc acgcagtgcc
     1381 cagcccggcc gacatcaaca gcgccgtcag gacggcgcgc ggagaattga atgactgcat
     1441 cacgtgctcc tctcttcctc gttcccgttc atgcggcagg gattcccgcc gccctgcagc
     1501 cagtctgcac ctgcagtggt tccaggatgg ttaaccgtcc ggttacggcg cgcccgccgc
     1561 gccgaccgct cgccccctgc ctgacgcccc gacgggattg cactatcatc tccgcgtgtt
     1621 tcctccccct tcggaccgtc ccatgaacac cccgcaagcc ggcatcctgc tccctgttcc
     1681 gcccctcgcc cgttacctgg gcttcaccct cgaaccggac agtgacgtcc atgccgggct
     1741 gtccgccctg cttgacgtgg tcgatggtga acgcacggtg gcgggcctcg ggctggccac
     1801 cgtgacggcc gctggcctca gcgtgccggg cctgcacgcc ttcccgacac cgagcgcgcc
     1861 cggcatcgaa ctgccggcca cgcccgcggc cctgtggctg tggttgcggg gtgaggatcg
     1921 gggcgaactg catctgcggg cgcagcggat cgaggcgctc ctggcaccgg tgttccgcct
     1981 cgtggacagc atcgacgcgt tcaagcacga caccggacgc gatctgtcgg gctatgagga
     2041 cggaaccgag aacccccagg gtgacgatgc cacaaccgcc gccattgccg ccgacggctc
     2101 cagcctggtc gccgtacagc gctggctgca cgacttccgc cgcctcgcgg ccatggcccc
     2161 cgaggcgcgc gacgactgca tcggccgccg tcgcagcgac aacgaggaac tggacgacgc
     2221 gcccgcgtcc gcccacgtca agcgcacggc gcaggagagc ttcagccccg aagctttcct
     2281 gctgcgtcgc tccatgccct ggagcgaggg tcgcgatgcc ggcctgatgt tcgtcgcctt
     2341 cggcgccacg ctcgatccgt tcgaagccca gctacgccgc atgaccggcc aggaagacgg
     2401 catcgtcgac gccctcttcc gcttcacgcg ccccctcacc ggagcctatt tctggtgtcc
     2461 gccggtgcgg gacgggaagc tgtgcctcat ccggtgacgg ggcgcctgcc tgggccgacc
     2521 gcggatggcg ggacgggtgt gccaagcggc gccggccacc cgttcagccc gacctgaccc
     2581 agcagcgtga cgcctgtcct ggcaacccgt cctgcgcgtc gccggacacc ctcacgatcg
     2641 tccaaacctc cgcatcgccg caagggccgc catggaaaag accatgagcg atccgacagg
     2701 cggttcggga atggtgtcga tggcgccgga gacataaacg tattcaaacc cgcgcgcgct
     2761 ctgacagaaa tcgccaacct gcgtctgcat ggtcgttccc gaacagacgc cccctggcga
     2821 catctggtag ccatccatcg ccttgatggc gatcagattc tcaccaccgt aaaaatcatc
     2881 cttgtatagc gaaaggtcga gaacgatcgg cacgggctgg ccactggcgt ccatgtgcat
     2941 gtccagcaag ccgaatgtaa tcagggtgcc gttcagatag agctcgaagt aatcgtcggc
     3001 aatgattttg agctggcccc cggccaccct gaaatccgag ttcagattga acaccgtcga
     3061 aaaataggca agccccgtcc gggtattgga cgcacacccg cctggcgggc agtaccacat
     3121 catgtcctct gcggccaggg cggtcgtcgc cgagtttcca taggggtgct gtgcatcgac
     3181 ggcggcggga ttattgccat ccaggcccct gatccagttc tcgccaagcg tcgcgttgtc
     3241 ggtcgccatc cacagatagt cggtccgcgc gctcagtgga taggtctgat acggccccgc
     3301 gttggcggca ccgaccagcc ccgagaccaa cagggacgcc acgacgatgc gcattgctct
     3361 caagatatcg gtcatttcgc caggactcct catgggcggc ccatcgccgc tgtgcgatgt
     3421 cttcacgccc ctcccggcac ggcgcaccga aaagggagtt ccgctcatct tcgccaggcc
     3481 gcggtttcaa atcggttacg gcatcgcccg cgccacgccc gtcggtgccg gcagagaggt
     3541 cgcaacgccg ttgctgcgag caccggacgt cccctcgtca cgcgccattc aactcgctcg
     3601 tgcaggcttc gccgacgacc cgtacggcgc cttgttcccc gcacccggcg atccgtgcct
     3661 gcacggcctg cttcaagccc atcagcccct ttcgaaatgc ggccacccag gggaggttgt
     3721 cctccatggg caggaatgat tcagcctggg ccgccagagc ctccctcatc agaccgacat
     3781 ccgcccccgg catcaacgcg gcgcgggcca cgttgcggaa ttccagcgcg tcgacctgca
     3841 cgagacccgc cggcagactg acccgcccct gcttcacgtg cacccaattc acctccggcg
     3901 gcccctcgca tcccagacgg cgcagacgcc acagggccac tttcaggttc tggcgcgcct
     3961 gggcgccatc cgaatccggc cacagcagat cggccaggcg gtccaccgac accttgctgc
     4021 cgccttcgca gatgagggcc atcagcaggg tcttcgtgcg gctgcctttc cagtcccgat
     4081 caaagatccg gcgcccgccg atctccaccg aaaattcacc gagggtccgg atgcggaccg
     4141 ccgcagtggt cgcgtcgcac cggtccccca tcgggaagag ccggacacgc agcgtctcca
     4201 gccagacgcc cgatcggttc aagggtcgca gggattcacc gggcggtaga gccgccagtg
     4261 ccgcgaacca cgcccgccgc gcccgatcca gttcaccggc ccgcacatcc cgcgcagcca
     4321 actcaaggtg cgccacggcc gccagacgcg ccatgccgca cgccgaccag gtcgggatcc
     4381 actgcgcggc ttcggtgcgc agggacgccc cggcatccgc cagatcggcc agcgcctgca
     4441 gacgcagcag gcggccgatg tacccgggca gcagggcgcc ggcgcgctca ccgacaccga
     4501 tgcaggcatc cgcatggatg cgggcccgca gggcgtcgcc ttggcgcagc gccatggccc
     4561 ccagcacata atggcgacag gcgtgcagga gcaggcgcgg caccggaacc gtccggtcgg
     4621 cgacggcccg tgaccagtcg gccacccgat ccgtatcgcc cacgtcggcc gccgcgtgca
     4681 tgcccagact ccagaccagc acctggagcg attcgggcag gccttcgaag gcggccgctg
     4741 cgcatgtgcg ctgcagcagc gccagcgcgt cgctactccg gtccaacatc agatggatca
     4801 ggctcagggc caccgtcacc agtaaacagg gcaggcggct gcgcgccttg acctcacaca
     4861 acacccaggc gtcactgaca agcacgtcgg cgcgggtgac cgctcccgcc agacactcgg
     4921 ccacgccccg cgcgcccgtc agcgccagct gcagcggttc cgaggcggcc cgctcggcct
     4981 ggtgcacccc gtcatcccag atcgtccagg cggcctgcgc atcgccgcgc agcaccaggg
     5041 ccacgagccc ccgccgcagg gcgagcgccg ccgcagcctg cgccgacggc cccccgcagg
     5101 gccgcgtggc cagagccgcg aggcgcgctt cccactccat cagctgcgcg catgcacgac
     5161 cgtcggcgac gatgccgtcc accgctgccg ccccgaccag ggccgcggca tccgggtcgg
     5221 cctcgctcaa cgcttccgca aagagggccg cccacggctc gccgcccggt gctcggccgg
     5281 caagagtccg cacgatgcgc tcccgcaacc cgcctgcggt gtcggacgca cggttcaagt
     5341 ccaaccccgt tgcggccgca gcagccgcgt caagatcaaa ccagtgcccg aaggcccgcg
     5401 caagcatgcg cgcgcgcccc tgcgactgac cggtggtgat gatttcagga caatgcgcca
     5461 tgatttcact cccgtttggc agtaaatggc tttatcctga tttcattctt gacgcccctg
     5521 acggatcggc cttgacgccc gtcaataacc atgaagtaac cacggttatt cagggatgtt
     5581 ttacataaaa tgatgaattc ccggcaaaaa ccgctctcgc caccggttgt tgaaggcgac
     5641 gcaccccttc ggcccgatgc ccgggatctc tggcaactct ggttcgatgg cgcagcccac
     5701 cccaacccgg gacgcctggg actgggcggc gtgctcattg cgccggacgg cagacgccac
     5761 gcctattccg ccctggcgcc ggaatgtggc tgcaacaacg aagcggaact gctcgccctg
     5821 ggccgggggc ttgagctggc ccgtggcgca ggcgccaccc atgtgcaggt ctggggcgac
     5881 agcgatttcg ccgtccgcca cctgaacggc gccgcccaca cggacatccc gcggctgcgc
     5941 gccttgctgg acgtcatcgg ccgtcagctg accggcttca ccctggtgca cctgcactgg
     6001 attccccgcc accgcaacca cgacgccgac cggctgagtc gcgcggcgct gggcctgccc
     6061 gacaagccgg cgccgcggcc ggtttcggcg cgcaagcggc gcggacgccg ctgaatcaac
     6121 gcccgggcac ttcctcggcc agcaggcgcg cggcaatgag gccattgccc agcccgaata
     6181 ccgtcgcggc cagggcaaaa accggtacca gatagaacac cccgtcgtgg ggcaccagcc
     6241 acaggcgcgc caccagcact tgcccgccga tatgggcaaa ggccgccagc acgctctggg
     6301 tcaccggacc gaaccagcgc cgcggcagat gcatggccgc ccccagcacc gccaggctgg
     6361 ccagtccgcc ggccagactg aggaagaagc ccggcgcaag gaactgcccg agcagcaggc
     6421 tgcctgccag gatgcgcagg attgccaccc aggccgcatc gcgccagccc cagcgcgcca
     6481 gcaccaccag ggtgatgatg ttggcaagcc ccggcttgac gcccggcaac ggcagcggga
     6541 tcgccgcctc cgccacggtc aggacgatgg ccgccgccgc atgacgggcc acccggtggt
     6601 cgtccgcggt gggcaccagg cggatctcag tagttgaggg agtcataggc ggcattacgg
     6661 cccgacaggg acaggctcac ttcattgggg gcgcagatgg ccaccgcgcc ggcggaatgc
     6721 agccagccct ggcgcacaca gtactgccgc ggccccggat cggcggccac ccgcgcccgg
     6781 ccaggctgga tctcgatgcg cgtggtgccg atcgggccgg gcacctcgac gatgcgcggc
     6841 gcgctcaggt ccacctcggc aaacacgcgc ccgcccgcct tgatgaccgc cttgtccggt
     6901 gccccgccct gccacaggac cagcgccgag gccgccgacg cgcccaggcc gagggccagc
     6961 accagcacgt cccccacacg caggtaggcg acccacgccg ccagccacgt gcgcaggtcg
     7021 gccaggcggc tcatcggcgg aaggagcggt gccgcaccag cacccgcgcg ctctcgcgga
     7081 agcgcccggc aagctgctcg gccacataca ccgaacggtg ctggccaccg gtgcagccga
     7141 tggcgatggt gaggtagctg cggttgtcgt gcacataggc cggcagccag gacgcgacga
     7201 aggcgtagat gtcctccacc atgcggccga cctcaggcac ccgttcgagg aagtcgatca
     7261 ccggcgcgtc aagccccgtc agcggacgca gctccgggtt gtagtgggga ttgggcaggc
     7321 agcgcacgtc gaacaccagg tccgcgtcca ccggaatgcc gtgcttgaac ccgaaggact
     7381 ggaacagcag ggtgatctgg ctgctggcct ccaggtccat gaaggatttg acccagttgc
     7441 gcagggtgtt ggccgagatg tcgctggtgt cgatgcgctg gcccagttcg gagatcagcg
     7501 ccagcgcctc ccgctcgcgg ccgatggcct ctgccagggt ggagcactcg tcccccaggg
     7561 ggtgcttgcg gcgggtttcg gaaaaacgcg cgatgagcgt ctcgtcgcgg gcttcgagaa
     7621 acaggaagcg caagtcggtg accatgcctc gcaacagcgc gacctgctgg ggcaacgcgg
     7681 caatacttgc gcccgagcgc acgtccacgg ccacggcgac gcgctgatag ccgccgcccc
     7741 gcagattgga aatcagctgg ggcaacagcg tcgatggcag gttgtccacg acatagtggc
     7801 cactgtcttc catgacgttg agggccacgc tcttacctga tccggacagg ccactgatga
     7861 gtacgagttg catggggcga tggatgtggg ggctggagga gtgggcggcg tcggtctgta
     7921 caagcctctc gcgccggcgc aaccgtacgc tgcggtacgc cgggtcaatg cccaatcata
     7981 cgcatgctcc gtgacggcac gcgcattttg tgaacgagcg tggaacgctc aggcgtccgg
     8041 cggcggcgcc tcgaccgccg gcagcgtgtc cgtcgcacac ggggccggtt cgctgtcacc
     8101 gtagatctcg tcggcgctgc gtccgcaacc gacgcaatag ccgctctcgt cgatctcgca
     8161 aatccccacg caggggttct cgtcactcgt gtccatgctg tcctcgtggg tctttcggcg
     8221 acatcgccgg cgcgcgatac ccggcccggc ctcgcggatc atcgcgcccc ctgaccgtga
     8281 cacaggtggc gcacggacgc aatgagccgc cccgggccgc gttctgcgcg acgggcgcgt
     8341 gattttctgc gcgctcccgg attctaccca aattcacaat agcgccagaa aaccaacgaa
     8401 ctcaccacaa tcttcaaaac cgtgcgtgag ccttcgctgg atgcagaacc aaccatccca
     8461 tttatctgga gctgtccgcc atgaagatca agaccctcgc cgccgccctg ctctccctga
     8521 gcgccctgca tgcccacgcc accgtgatcg acttcaacca ggtcatcggc ttccagaccc
     8581 tgccctcgcc ctatcaggaa gacggcttca cgatcaccaa cagcagcacc aacagctccg
     8641 cgatgctgtt ctggggcacc ctgtcctaca acgccgaccc caacggcaac acctacagcc
     8701 acaactacgg cggcacgacc agcacgctga gcaagaccgg cggcggcagc ttcaccttca
     8761 actccatcga cctgaccgac gtttacaact tcggttccgg cggcagtgtg gtgttcgact
     8821 tcctgtacag caacaacagc accagccaca gcaccgtcaa cctgcccacg gtggcaggcc
     8881 tgcacacctt caccttcgac ctcgccaacc tcacctcggt ttcgtggacg ccggtgggca
     8941 cccagggcaa ctggatccag gtggacaaca tcgtgctcga caccaccctc agcaatcgcg
     9001 tgcccgagcc caccagcatg gccctcgtcg gcctcggcgt ggccatggcc ggcgccctgc
     9061 gccgccgtcg ggccgcctga cccgtcgaac gccgcacgaa gggtgcctcc acgagggggc
     9121 accctttttt catggagtgg acgctgccgg tggcggcacg acgtccgcgg atgccttgtc
     9181 ggccggcgcg ccttccggcc agcgacgctg cgccagccca tccaggatgg cctgccggcg
     9241 gccatcgccc gcggcgcccc actcggcgat ttcggtcagg gtgcggcgac agcctgcaca
     9301 ccagccagtc attggatcga tgcggcagat gccggtacaa ggggatgcga cgctcatggg
     9361 gttccgtcag aaagggccgg ccaccgctcg gacatgggcc ggcaggaaat gggctcaggc
     9421 cgcacagccg cggcggccac ggtgggcgag gctcgcccgc gccacgcgcg aagcgggcgg
     9481 acgccccagc tgggcgctga tccaggcggc gatgtccacc agcgcctcca gatcgacgcc
     9541 gctggcaatg cccagcccgt tcagcagata gaccagatcc tcggtcgcca ggttgccgga
     9601 cgcgcccgca gcgtagggac agccgcccag accgcccacc gacgcatcga acacggccac
     9661 accggcctgc agcgaggcat agacgttggc cagggcctgt ccataggtat cgtggtaatg
     9721 cccggcgagc tgctccacgg gcacccgctg cgccaccgcg tcgagcatcc gtcgggccgc
     9781 gccgggcgtg ccggtgccga tcgtgtcgcc caggctcacc tcgtaacagc ccatgtcgta
     9841 cagcgtgccc gccacctcgg ccactgccga tggggccacc gcgccctcat aaggacagcc
     9901 cagcacgcag gagacatagc cgcgcacccg cacgccggcc tcatcggcgg ccgccatgat
     9961 cggttcgaag cgggccaggg attccgccac cgagcagttg atgttgcgcc ggctgaagga
    10021 ttccgacgcg gccgcaaaaa ccgccaccga cttcgccccg gccgccagag ccgcgtcgaa
    10081 gcccttcagg ttgggtgtca gcaccgggta gtccacaccg gggagacgtt cgatgcgggc
    10141 cagcacctcg gccgcgtccc ccatctgggg cacccacttc ggcgacacga aggacgtggc
    10201 ctccacggcc ggcaggccgg cccgggcgag ccggtcgatc agggccacct tggtgtccgt
    10261 ggcgatcacc tgggcctcgt tctgcaggcc gtcgcggggg cccacctcga cgatgcgcac
    10321 ccgcgcgggc aaagtcatgt cgggtccctt catgctgcgt caaactccac cagttcggcg
    10381 ccgtccgcca cctgatcgcc cacggcgaag cggaagccct tcacggtgcc cgccgtcggc
    10441 gcggcgatgg tgtgctccat cttcatcgcc tccaggatca gcagcggcgc gcccttgtcc
    10501 acccgcgccc cttccgccgc cagcagggcg atgaccttgc ccggcatcgg cgccagcagc
    10561 ccgccctccg cgccgccgcc ctccccgctg aagaacaggg gatcgacgca ggcgaacacc
    10621 cgcgcgcgcc cgtgggcaaa cacatgacgc cgctcgccgg ccgcgaccac ggtcgccgcc
    10681 agacgccggc cgccaaattc gccgcgcagc agtccgcgcg gattgaggct gccggacacc
    10741 gtcgtcgcga ccccgtccag ggtgatctcg aaggcgccgg gcagggtcgc cacgcggagg
    10801 gtcttctgcg cttcgccatg gcggaagatc aggctgcggg ccggcgctgc gttcatgcgc
    10861 cagccgtcgc gggcgtgcca cggcgaggag ggatcggcgc tgcgggccgc ggtggcagcc
    10921 gcccgttcct gttcccgcaa cagctcggcc agggccaggg ccagccactc atcccgctcc
    10981 ggcgcggacg cttcggggaa gagataggcc tgctcccgct cgatgaggcc ggtgtccagg
    11041 tccgcattgg caaaggccgg gcaggcggtg agacgggaca ggaactcgat gttgttggcg
    11101 acgcccgcca cccggtagtc cgccagggcc tggagcatgc gcgccagggc ccggtcccgg
    11161 ttcacgtccc acacgatgag cttggcgatc atcgggtcgt agtggggcgt gatctcgtcg
    11221 ccctcctcca cgccggtatc cacgcgcacg tgcagacctt cgtccggcgg cgccagatgc
    11281 agcagtcggc cggtggcggg gaggaagccc ttgcccggat cttcggcgta gatgcgcgcc
    11341 tccagggcgt ggccgttgag agcgagctgt tcctgggcga ggggcagggg ctcgccggcc
    11401 gccacgcgca gctgccattc caccagatcg aggccggtga tcatctcggt gaccggatgc
    11461 tccacctgca ggcgggtgtt catctccatg aaatagaagc tgccgtcctg ctgggcgatg
    11521 aactccaccg tgccggcgcc cacgtagccc accgcgcggg ccgcctccac cgccgcccgt
    11581 cccatcgcgg cgcggcgctc cggcgtcatg cccggcgcgg gcgcttcctc cagcaccttc
    11641 tggtggcggc gctgcaccga gcagtcccgc tcgaagagat acacggcgtg gccatgcgaa
    11701 tcgccgaaca cctggatctc gatgtggcgc gggcgcagga tgtacttctc caccagcacg
    11761 tggtcgtcac caaagctgga cgccgcctcc cgcttgcacg aggccagcgc gtcgaggaag
    11821 tcggcgctgc actccacccg ccgcatgccc ttgccgccgc caccggccgc cgccttgatg
    11881 agcaccggat agccgatggc gtccgcctcc cgctgcagcg tagccggcgt ctggtcgtcg
    11941 ccgtggtaac ccggcgtgag gggcacgccg gcctgctcca tcagcttctt ggcctcggac
    12001 ttggagccca tggcgcggat cgccgccacc ggcggaccga tgaacacgat gccggccgcc
    12061 tcgcaggctt cgcagaaggc ctcgttctcc gacaggaagc cgtaacccgg gtggatcgcc
    12121 tgggcgccgg tccgctgcgc cgcctcgatg atgcgctcga tcaccagata actctcccgc
    12181 gcggcggcgg gaccgatcag caccgcctcg tcggccaggc gcacgtggcg tgcaccggcg
    12241 tcggcttccg aatacaccgc caccgtccgg atgcccaggc ggcgggcgg
//
LOCUS       DAOQPA010000105        12127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2682_length_12127_cov_20.986746, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000105 DAOQPA010000000
VERSION     DAOQPA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_12127_cov_20.986746"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PKX01_13365"
     CDS             complement(<1..1282)
                     /locus_tag="PKX01_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HMZ77166.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAEVL
                     RACAARSDELRKELTNFINEHTPRVEGTEDIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLQRQNISRLDVVNFISHGITKAPQSGASPARPNESSEQ
                     GEAEPENPSAGGALDSFTQNLNQQALLGKIDPLIGREKEVERVIQTLCRRRKNNPLLV
                     GEAGVGKTAIAEGLARRIVEGRVPEILADAQVYALDMGALLAGTKYRGDFEQRLKAVL
                     KQLVDHNNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGQLKCIGATTYTEFRQ
                     IFEKDHALSRRFQKVDVVEPSVPETVEILKGLKSRFEQHHGIKYSASALSSAAELSAR
                     YINDRHLPDKAIDVIDEAGAAQRILPKSKQKKLIG"
     gene            complement(1284..1592)
                     /gene="clpS"
                     /locus_tag="PKX01_13370"
     CDS             complement(1284..1592)
                     /gene="clpS"
                     /locus_tag="PKX01_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HMZ77167.1"
                     /translation="MVTKKQGESVLETEVTRIKPPPLFKVMLLNDDFTPMDFVVTVIE
                     KFFGLDREQATRIMLKVHREGVGVCGIFPRDIAATKVEQVIAYARQNQHPLACVMEEN
                     "
     gene            2003..2206
                     /locus_tag="PKX01_13375"
     CDS             2003..2206
                     /locus_tag="PKX01_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMZ77168.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGSEDLFAHFSAISMSGFKSLKE
                     GEKVQFEVTQGPKGKQASNIQRA"
     gene            complement(2457..2624)
                     /gene="rpmG"
                     /locus_tag="PKX01_13380"
     CDS             complement(2457..2624)
                     /gene="rpmG"
                     /locus_tag="PKX01_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMZ77169.1"
                     /translation="MAKGGREKIKLESTAGTGHFYTTTKNKRTTPNKLEFSKYDPVAR
                     KHVPYKEVKLK"
     gene            complement(2653..2889)
                     /gene="rpmB"
                     /locus_tag="PKX01_13385"
     CDS             complement(2653..2889)
                     /gene="rpmB"
                     /locus_tag="PKX01_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMZ77170.1"
                     /translation="MARVCQVTGKAPMVGNNVSHANNKTKRRFLPNLQNRKFWSEAEN
                     RWVRLRVTNAALRTIDKKGIDTVIAELRARGEKV"
     gene            complement(2986..3663)
                     /gene="radC"
                     /locus_tag="PKX01_13390"
     CDS             complement(2986..3663)
                     /gene="radC"
                     /locus_tag="PKX01_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMZ77171.1"
                     /translation="MAITDWPADERPRERLLAHGAEVLSDAELLALFLRVGVRGKSAV
                     DLARDLLGRFGSLSALCTASVDDFSSIPGMGPAKYAQLQAVLELARRALGEEMATRDV
                     LDSPRAVRDWLRLKLAGLPHEVFMVLLLDAQNRVIASEELFRGTLAQTSVYPREVVKL
                     ALLRNAAAVILAHNHPSGVGEPSHADQLLTRSLTQALALIDVRVLDHFIVAGTASPVS
                     FAERGLL"
     gene            3710..4903
                     /gene="coaBC"
                     /locus_tag="PKX01_13395"
     CDS             3710..4903
                     /gene="coaBC"
                     /locus_tag="PKX01_13395"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMZ77172.1"
                     /translation="MAELSGRKIVLGVTGGVAAYKAAELVRLLGKAGAEVHVVLTEGG
                     ARFVTAVTFQALSGNPVWTDLWDPRMANNMAHIDLSRDADAILIAPATADVMARLAQG
                     RADDLLTTLCLARDCPLLVAPAMNRQMWEHPATRRNAVQLAADGVTLFGPAAGEQACG
                     EVGLGRMLEPEDLCEALIGFFQPKLLAGRRVVMTAGPTFEAIDPVRGITNSSSGKMAY
                     ALARACAQAGADVALVSGPVALPVPAGVARISVRSALQMRDAVFAALPGADVFIGVAA
                     VADYRPDTSAEHKIKKSGAALQISLTPNPDILAEVAARADAPFCVGFAAESRNLDEYA
                     EGKRRAKKLPLVVGNLVQDGLGSDDNQVVLYDEAGRHPLERAPKPEVARAIVAHLAGL
                     LPPLS"
     gene            4928..5377
                     /gene="dut"
                     /locus_tag="PKX01_13400"
     CDS             4928..5377
                     /gene="dut"
                     /locus_tag="PKX01_13400"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMZ77173.1"
                     /translation="MHSIDIRILDGRLKEQPPAYATSGAAGLDLRACLEAPAHLLPGA
                     TLLVPTGIAIHLADPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     HDSFTIQPMERIAQLVVVPVLQVAFNLVESFDVSERGAGGFGSTGKH"
     gene            5379..5807
                     /locus_tag="PKX01_13405"
     CDS             5379..5807
                     /locus_tag="PKX01_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ77174.1"
                     /translation="MSRRGIPTGVHVLFERDSRVLLMRRAGTGFFDGLYSLPGGHVEE
                     GESVGMTAHREMAEELGVTLAPDAVALLGVVHRRSDTNRIDFFVRALTWSGEPRIAEP
                     DKCDDLAWFGRDALPLALVPYVRDALAAGDGPWLMESGWD"
     gene            complement(5887..8559)
                     /locus_tag="PKX01_13410"
     CDS             complement(5887..8559)
                     /locus_tag="PKX01_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HMZ77175.1"
                     /translation="MQNSFQIRALAAAIGSALVLAACGGGGSTGYSQINGTAAEGTAI
                     ANSTLTVKDASGKTRSVTTDASGNYTLSLADLSFPFMLQITGSQGTWHALVSSDDVGK
                     PVNVNNATDSIAQLALGVSGNAALRAAFTAGSFKSVTGQAIASADSKLLDALEAELGK
                     RPESLRSATFTPATDSTPGDETDRLLTLVDAQPSGSGFATFNRVPEFVWTDSIVTKTY
                     DGTTDDLLTAGLGKTGLTSATAPAYVNAASPTAAELRRNAIHTNYRALVDANKTAGGW
                     TTLYGPNIDTSGNDTLGEGRIAGDETIAYSGDSSGKRKVTLMVQVPASFDKTKPCIVT
                     ATSSGSRGIYGAIGTAGDWGLKHGCAVAYTDKGSGNGMHDLAADTVNLIDGTASSSAS
                     AGKRALFAANLSKTELDAYNLKSPNRIAYKHAHSQQNPEKDWGRNTLDAVGFAFYVLN
                     EKYGTVSNGKKLRDLTPRNTLVIASSASNGAGAALQAAELDKLGLIDGVAVTEPQIQP
                     KDVSSLTIKQGSNTVATIGKPLIDYFTYANIYQPCAALATAASGSPGASLVAFYATNR
                     CTSLKEKGLVSGTTTQEQADDALQKLHAYGWAAEQDVLHASHHALATPSIVVTYINTY
                     GRFNVTDEVCGFTFATTVASGATLGNVTATSALVQAGIFATGNGVPPTAGINLVYNDS
                     VGGAKRDVLAISPSTNRADGALDGALCARSLVTGSDPVTGAKLTGTLLAQSQRVRQGI
                     DEVLFKGELGGKPAIIVSGRSDTLIPVNHASRAYYGMSQKADGTTSRLRYYEVTNAQH
                     FDAFIDNNAAVAGYDTRLVPLHVYFNRAMDLMYAYLKTGAALPASQVIRTTPRGGTAG
                     AAPAISATNVPAIATTPAAGDTITYSSGTLSIPD"
     gene            complement(8716..10488)
                     /gene="aceK"
                     /locus_tag="PKX01_13415"
     CDS             complement(8716..10488)
                     /gene="aceK"
                     /locus_tag="PKX01_13415"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HMZ77176.1"
                     /translation="MDTHGNENPVAQAIAQAMIEGFNKHYRIFRETSRHAKESFEAAD
                     WQDQLSAVRNRVQFYDDRVNETVQRLHEEFDAESIDDATWQAVKLHYIGLLLNHKQPE
                     LAETFFNSVTTKILHRDYFSNDYIFTRPAISTDYIESFPPTYSSYYPQDEGLRATVRR
                     IVEDFDWQRSFEDLERDVDYVLRTVETHLGGWPRMEVNCQIQVLYSAFYRNKTAYIIG
                     KGLNGYQEIPFALAVRHGASGMLYLDTILLDTWRISLLFSLSRAYFLVDMEVPSGYVQ
                     FLRSIMPNKPRSELYTMLGLGKQGKTSFFRDLVSHLRHSNDQFIEAPGIRGLVMLVFT
                     LPSYPYVFKIIKDTFGSSKNMDRATVKRKYLLVRQVDRVGRMADTLEFSNVALPRSRF
                     HPDLILQLRTLAPSVIEEDGDTLVVKHLYIERRMTPLNLYLDKASDEQVEEAVKEYGQ
                     AISELAIANIFPGDMLWKNFGVTRYGRVVFYDYDEIEYMTDCNFRRIPPAPNEDMEMS
                     GEPWYSAGPHDIFPEEFATFLLGQPKIRKAFLKHHRALLDPRFWQEAQEKIRNGYVED
                     FYPYPQALRFRTLYAERPDEPPPA"
     gene            10824..12068
                     /gene="icd"
                     /locus_tag="PKX01_13420"
     CDS             10824..12068
                     /gene="icd"
                     /locus_tag="PKX01_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ77177.1"
                     /translation="MSTSLIKVPAGGQKIVPGQPTPDNPIIPYIEGDGIGVDITPVMI
                     QVIDAAVTKAYGGQRKIHWMEVYAGEKSTQLYGPDEWFPKETFDALKEFSVSIKGPMT
                     TPVGGGIRSLNVALRQELDLYQCVRPVRYFKGVPSPLKQPELTDMVIFRENTEDIYAG
                     IEWAAESDNVKKVIAFLQQEMGVKKIRFPESSGIGIKPVSIEGTTRLVRAAINYAIAN
                     DRKSVTLVHKGNIMKFTEGLFRDTGYKVAQQEFGAQPIDGGPWCSFVNPRTGRTIIVK
                     DAIADAFLQQILLRPAEYDVIATTNLNGDYISDALAAQVGGIGIAPGANISDQYACFE
                     ATHGTAPKYAGLDKVNPGSLILSAEMMLRHLGWVEAADLVIKAMEAAIGDKQVTYDFA
                     RLMEGATEVSCSGFGEAMVARM"
BASE COUNT         1950 a         3550 c         4322 g         2305 t
ORIGIN      
        1 tgccgatgag cttcttttgc ttggacttgg gcaggatgcg ctgggcggcg ccggcttcgt
       61 cgatgacgtc gatggccttg tccggcaggt gacggtcgtt gatgtagcgg gccgagagtt
      121 ccgccgcgct ggacagggcc gaagcggaat acttgatgcc gtggtgctgc tcgaagcgcg
      181 acttgaggcc cttgaggatc tccacggtct cggggacgga gggctcgacc acgtccactt
      241 tctggaagcg ccgcgacagg gcgtgatcct tctcgaagat ctgccggaac tccgtgtaag
      301 tggtggcgcc gatgcacttg agctgcccgg acgacagggc cggcttgagc aggttggatg
      361 catcgagcgt gccgccggat gccgcgccgg caccgatcag tgtgtggatc tcgtcgatga
      421 acagtatggc gttgttgtga tcgacgagtt gcttcagtac cgccttgagg cgctgctcga
      481 agtcgccacg atatttggtg ccggccagca gggcgcccat gtcgagggcg tacacctggg
      541 catccgcaag gatctcgggc acccgacctt cgacgatgcg tcgcgccaga ccttccgcga
      601 tggcggtctt gccgacgccg gcctcgccga ccagcagggg gttgttcttg cgccgccggc
      661 agagggtctg gatgacccgc tccacttcct tctctcggcc gatgagcgga tcaatcttgc
      721 cgaggagcgc ctgctggttg agattttgcg tgaagctgtc cagtgcgccg cccgccgagg
      781 ggttttccgg ctcggcctca ccctgttccg aagattcgtt cgggcgcgcg ggactggcgc
      841 cggactgggg tgccttggtg atgccgtggg agatgaaatt caccacatcc aggcgggaga
      901 tgttctgtct ttgcaggaaa tagaccgcgt gagagtcctt ttcgccgaag atcgcgacga
      961 gcacattggc gccggtgacc tccttcttgc cggaggactg cacatggagg atggcgcgct
     1021 ggatcacccg ctggaagccg agcgtgggct gggtgtcgat gtcttctgtg ccttcgacgc
     1081 gaggcgtgtg ctcgttgatg aaattggtca gttccttgcg cagttcgtcg ctgcgggccg
     1141 cgcaggccct cagcacttcg gctgcggaag gattgtcgag aagcgcgagc aggaggtgct
     1201 cgacggtgat gaactcatga cgcttttgcc gggcttcgac gaaggccatg tgcagactga
     1261 cttcaagctc ttgcgcgatc atttcagttt tcctccatga cgcaggccag aggatgctgg
     1321 ttctggcgcg cgtacgcgat gacctgctcg accttggtgg ctgcgatgtc ccgggggaag
     1381 atgccgcaga cgccaacgcc ctctctgtga actttgagca tgatccgcgt ggcttgttcg
     1441 cggtccagtc caaagaattt ctcgatgaca gtcacgacga agtccatggg tgtgaagtcg
     1501 tcattcagca gcatgacctt gaacaagggg ggcggtttga tccgcgtgac ctccgtttca
     1561 agtactgatt cgccctgctt ttttgtgacc atgcggccca ttctaaacaa cctcgatgat
     1621 tgtgcaagag agaattgaca cacgatttgg cactgattcg tgttggtgca gaggccctcg
     1681 gatggtgcgt tgcacggcca ttttcagcgc tccggagtgc cgcgtccgcc ttgaaaggcg
     1741 ctgcgaaatt ttgacgcgat tggccgcgat cctgctgtat tgcagcatga aaatgctgcg
     1801 actggaccgt tgacggtggg gggcaagtga cgtaaaagcg aagccgtgtc tgcgtcggga
     1861 ggatggttgg cgctttgccg cgggggcttt tggggaaaag ccgtcgggca tgggtattac
     1921 gccaaccgtc cacgagcacc cgctgcagac tcagccggag acccggttct gtatgactga
     1981 aaaccaagaa ggaaactgca ctatggcaac tggtactgtt aagtggttca acgacgccaa
     2041 aggcttcggc ttcattacgc cggacgacgg aagcgaagat ctgtttgcgc acttttccgc
     2101 gatttccatg agcggattca agtcgctgaa ggagggggaa aaggttcagt tcgaggtcac
     2161 gcagggcccc aagggcaagc aggcgtcgaa tatccagcgt gcctgatctc tcgacgcacc
     2221 ctgggcactg ttttggtgca gtgtttgggg ggcgtgtgag gaagggttcg tgaaaaaccg
     2281 ccgggtgcat ctgcattccg gcggttttca tttgcaagat tgtgtcgcgg ttgacgatgg
     2341 atcagggggc ggtcaggccg taggggcgca aactggcgtt gcgccgcgca aagcaaaaga
     2401 cccgctctgg gcgggtcttt caggtgcaac aagcagtcat cgcgtaccgg gagcgattac
     2461 ttgagcttga cttccttgta cggcacgtgc ttgcgggcga cggggtcgta cttgctgaat
     2521 tccagcttgt tcggcgtcgt gcgcttgttc ttggtggtgg tgtagaagtg cccggtgcca
     2581 gcagtggact cgagcttgat cttttcgcgg ccgccttttg ccatggtggt ttatctccag
     2641 tgggtgaaag gctcagacct tctcgccacg agcgcgcagc tcggcgatga cggtgtcgat
     2701 gcctttcttg tcgatggtgc gcagggcggc gttggtcacg cgcaggcgca cccagcggtt
     2761 ctcggcctca ctccagaact tgcggttctg caggttgggc aggaaacgac gcttggtctt
     2821 gttgttggcg tgggagacgt tgttccccac catcggtgct tttccggtta cttgacagac
     2881 gcgagccatg tgttgctcca aacaattttg aatcggtaag cccttaagtc tatcaaagct
     2941 tgtgaagatg atgcaagcct ttgatggggc ggtcctatca gcacgtcaca gcaggccgcg
     3001 ttccgcgaag gataccggcg acgcggtgcc agcgacgatg aagtgatcca gcacacgcac
     3061 gtcgatgagc gccagggcct gggtgaggct gcgggtgagg agctggtcgg cgtggctggg
     3121 ttcgccgacg cccgatggat ggttgtgggc caggatcacg gccgcggcgt tacgcaggag
     3181 ggccagcttg accacctcgc ggggatagac gctggtctgg gcaagggtgc cgcgaaacaa
     3241 ttcctcgctg gcgatgaccc ggttctgggc gtccagcaga aggaccatga agacttcgtg
     3301 gggcagaccg gcgagcttga ggcgcagcca gtcgcgcacg gcgcggggcg aatcgaggac
     3361 gtcgcgggtg gccatctcct cgcccaaggc gcggcgggcg agttccagga cggcctgcaa
     3421 ctgggcgtac ttggcgggcc ccatgccggg gatcgaggaa aaatcgtcca cggacgcggt
     3481 gcacagggcc gacaggctgc cgaagcggcc gagcaggtcg cgggcgaggt ccaccgcgct
     3541 cttgccgcgc acgccgacgc gcaggaagag ggcgagcagt tccgcgtcag acagcacttc
     3601 ggcgccatgg gcgagcaggc gttcccgcgg gcgttcgtcc gcgggccagt cggtgatggc
     3661 catcggtgta gaatccggat tccagaggat tgcggatcat accatcggca tggctgaact
     3721 ttcgggcagg aaaatcgtgc tgggcgtcac cggcggcgtg gcggcctaca aggcggcaga
     3781 actggtgcga ctgctgggca aggcgggggc ggaggtgcac gtggtgctca ccgagggggg
     3841 ggcgcgcttc gtcaccgcgg tgaccttcca ggccttgtcc ggcaacccgg tctggaccga
     3901 cctgtgggat ccgcgtatgg ccaacaacat ggcccatatc gacctgtcgc gggacgcgga
     3961 cgcgatcctc attgcgccgg ccaccgccga cgtgatggcg cgtctcgccc agggccgcgc
     4021 cgatgatctg ctgacgacgc tgtgtctggc gcgggactgc ccgctgctgg tggcgccggc
     4081 gatgaaccgg cagatgtggg agcatcctgc gacccgccgc aacgccgtgc aactggcggc
     4141 ggacggggtg accctgttcg gtccggcggc gggggagcag gcctgtggcg aggtgggcct
     4201 tgggcgcatg ctggagccgg aggacctgtg cgaggcattg atcggcttct tccagcccaa
     4261 gctgctggcc ggccggcgcg tggtgatgac cgcagggccg accttcgagg ccatcgaccc
     4321 ggtgcgcggg atcaccaatt caagttccgg caagatggcg tatgccctgg cccgcgcctg
     4381 cgctcaggcg ggggcggacg tggccctggt gagtggcccg gtcgcgctgc cggtgccggc
     4441 cggcgtggca cggatcagcg tcaggagcgc gctgcagatg cgcgatgcgg tgttcgccgc
     4501 gctgcccggt gcggacgtgt tcatcggcgt tgctgcggtg gccgattacc ggccggatac
     4561 gtcggcggag cacaagatca agaagtcggg tgcggccctg cagatcagcc tgacgcccaa
     4621 tccggacatc ctcgccgagg tggccgcgcg cgcggatgcg cccttctgcg tcggctttgc
     4681 ggcggagagc cggaacctgg atgaatacgc ggaaggcaag cgacgcgcca agaagctgcc
     4741 cctggtggtc ggcaatctgg tccaggatgg tctggggagc gacgacaacc aggtcgtgct
     4801 gtatgacgag gcgggtcgtc acccgctcga acgggcgccc aaacccgagg tggcccgcgc
     4861 catcgtcgcg catctggcag gcctgttgcc gccactttcc tgaattcgtc agcggagact
     4921 cacacccatg cacagcatcg acatccggat cctcgacgga cgcctcaagg aacagccccc
     4981 ggcctacgcc accagcggcg ccgcggggct cgacctgcgc gcctgtctgg aggcgccggc
     5041 ccatctgctg cccggcgcga cgctgctggt gccgacgggc atcgcgatcc atctggccga
     5101 ccctggcctg gccgcgatga tcctgccccg ctcggggttg gggcacaagc atggcatcgt
     5161 gctgggcaat ctggtgggct tgatcgattc agattaccag gggcagatct tcgtgtccgt
     5221 atggaatcgg ggccacgaca gcttcacgat ccagcccatg gaacgcatcg cccagctggt
     5281 ggtggtgccg gtgctgcagg tggccttcaa tcttgtcgag tcctttgacg tcagcgagcg
     5341 tggcgcggga ggcttcggca gcaccgggaa gcactgattt gagtcgtcgc ggcatcccca
     5401 ccggcgtgca tgtgctgttc gagcgggaca gtcgggtgct gttgatgcgc cgggccggca
     5461 ccggcttctt cgacggactc tacagtctgc ccggcggaca cgtggaggag ggggagtccg
     5521 tcggcatgac agcccatcgc gagatggccg aagaactggg tgtgaccctg gcgccggacg
     5581 cagtggcgct gctgggggtg gtgcatcgcc gctcggacac caaccggatc gacttcttcg
     5641 tgcgggccct gacgtggtcc ggtgagccgc gcattgcaga accggacaag tgcgacgatc
     5701 tggcctggtt cgggcgcgac gcactgccgc tggcgctggt gccctatgtg cgggacgcgc
     5761 tggcggccgg tgacggccca tggctgatgg agtccggctg ggactgagcg gagcgccgcg
     5821 aggcgattca gacgaaaaag ggcgaagccg tggcttcgcc ctttttcatg ctgcgtgcga
     5881 tacggatcag tccggaatgc tgagcgtccc gctgctgtag gtgatggtgt caccggctgc
     5941 cggggtggtg gcgatggcgg gcacgttggt ggcgctgatc gccggtgcgg cgccagccgt
     6001 accgccccgc ggcgtggtgc ggatcacctg ggaggcgggc aacgcggcgc cggtcttgag
     6061 gtaggcgtac atcaggtcca tggcgcggtt gaaatacaca tgcaggggaa caagccgggt
     6121 gtcgtagccg gcgacggccg cgttgttgtc gatgaaggcg tcgaagtgct gggcgttggt
     6181 cacctcgtag tagcgcagcc tgctggtggt gccgtctgcc ttctgactca tgccgtagta
     6241 cgcgcgggag gcgtgattga ccgggatcag ggtgtcggac cggcccgaca cgatgatggc
     6301 cggcttgccg cccagttcgc ccttgaacag gacctcgtcg atgccctgac gcacgcgctg
     6361 ggactgggcg aggagcgtgc ccgtgagctt ggcgccggtg accgggtcgc tgccggtgac
     6421 cagcgagcgg gcgcacaggg cgccgtccag ggcgccgtcg gcccggttgg tggagggcga
     6481 gatggcgagc acatcgcgct tggcgccgcc gaccgagtcg ttatagacca ggttgatgcc
     6541 ggctgtgggc gggacgccgt tgccggtggc gaagatgccg gcctggacca gggcgctggt
     6601 ggcggtgacg ttgccgaggg tggcgccgga ggcgacggtg gtggcgaagg tgaagccgca
     6661 cacttcgtcg gtcacgttga agcgcccgta ggtgttgatg taggtgacga cgatggaggg
     6721 cgtggccagg gcgtgatggg aggcgtgcag cacgtcctgc tctgcggccc agccgtaggc
     6781 atggagcttc tggagggcgt cgtcggcctg ttcctgggtg gtggtgccgc tcaccagacc
     6841 cttttccttg agggacgtgc accggttggt ggcgtagaag gcaacgaggc ttgcgccggg
     6901 cgacccgctt gctgccgtgg cgagggcggc gcagggctgg tagatgttgg cgtaggtgaa
     6961 gtagtcgatg aggggcttgc cgatcgtcgc gacggtgttg ctgccctgtt tgatggtgag
     7021 gctgctcacg tccttgggct ggatctgcgg ttcggtgacg gccacgccgt cgatgagccc
     7081 gagcttgtcc agctcggccg cctgcagggc ggcgccggcg ccgttggagg cggaggaggc
     7141 gatgacgagt gtgttcctgg gggtcagatc gcgcagcttc ttgccattgc tgacggtgcc
     7201 gtacttctcg ttgagcacgt agaaggcgaa gcccacggcg tcgagggtgt tgcggcccca
     7261 gtccttctcg gggttctgct gggagtgggc gtgcttgtag gcgatgcggt tggggctctt
     7321 gaggttgtag gcgtccagtt cggtcttgct cagattggcg gcgaacaggg cgcgcttgcc
     7381 ggcgctggcg ctgctgctgg ccgtgccgtc gatgaggttg acggtgtcgg cggccaggtc
     7441 gtgcatgccg ttgccgctgc ccttgtcggt gtaggcgacg gcgcagccgt gcttgaggcc
     7501 ccagtcgccg gcggtgccga tggcgccgta gatgccgcgg gagccggagg acgtggcggt
     7561 gacgatgcag ggcttggtct tgtcaaagct ggccggcacc tggaccatca gcgtgacctt
     7621 gcgcttgccg ctggagtcgc ccgaataggc gatggtctcg tcgccggcga tcctgccttc
     7681 gcccagggtg tcgttgccgc tggtgtcgat gttggggccg tacagggtgg tccagccgcc
     7741 ggcggtcttg ttggcgtcca ccagcgcacg gtagttggtg tggatggcat tgcggcgcag
     7801 ctcggcggcg gttggcgagg ccgcattgac gtaggccgga gccgtggcgg acgtcaggcc
     7861 ggtcttgccg aggccggcgg tgagcaggtc gtcggtggtg ccgtcatagg tcttggtgac
     7921 gatgctgtcg gtccacacga actcgggcac gcggttgaaa gtggcgaagc cgctgccgct
     7981 gggctgggca tccacgaggg tgagcaggcg gtcggtttcg tcgcccggag tgctgtcggt
     8041 ggcgggggtg aaggtggcgc tgcgcaggct ctccggccgc ttgccgagtt ccgcttcgag
     8101 ggcgtccagc agcttgctgt cggcggaggc gatggcctgg ccggtgacgc tcttgaaact
     8161 gccggcggtg aaggccgcgc gcagggccgc gttgccagag acgccgaggg cgagctgggc
     8221 aatgctgtcg gtggcgttat tgacgttgac cggcttgccc acgtcgtcgc tgctcaccag
     8281 cgcatgccag gtgccctggc tgccagtgat ctggagcatg aaggggaagc tgagatcggc
     8341 gaggctgagc gtgtaattgc ccgaggcatc ggtggtcacg ctgcgggtct ttccgctggc
     8401 atccttgacg gtcagcgtgc tgttggcgat ggccgtgcct tctgccgccg tgccgttgat
     8461 ctggctgtag ccggtgctgc cgccgccacc gcaggcggcc aggacgaggg cgctgccgat
     8521 ggcggcggca agggcccgga tctgaaagct gttttgcacg attgtctccc ctattggtat
     8581 ggatgccgga ttgctgcgag ggtgtcgcat cggcatgcat agtgggcgcg aaatccaggc
     8641 gcggccactg gggtttacgt gaatagggtc agccctgatg gccgtccgcc gggaacggcg
     8701 gctggggcga cggcgtcacg ctggcggtgg ttcgtcagga cgctcggcgt agagcgtgcg
     8761 gaagcgcaat gcctgcggat acgggtagaa atcctccaca tagccgttgc ggatcttctc
     8821 ctgggcctcc tgccagaacc tgggatcgag cagggcccgg tgatgcttga ggaaggcctt
     8881 gcggatcttc ggctggccga gcaggaaggt ggcgaactcc tcggggaaga tgtcgtgggg
     8941 cccggcggaa taccagggct cgccggacat ctccatgtcc tcgttggggg cgggggggat
     9001 ccgccggaag ttgcagtcgg tcatgtactc gatctcgtcg tagtcgtaga agaccacgcg
     9061 gccgtagcgg gtgacgccga agttcttcca cagcatgtcg cccgggaaga tgttggcgat
     9121 ggccagctcg ctgatggcct ggccgtactc cttgacggcc tcctcaacct gctcgtcact
     9181 ggccttgtcc aggtagaggt tgaggggcgt catgcgccgt tcgatgtaca ggtgcttgac
     9241 caccagggtg tcgccgtcct cttcgatgac cgacggggcg agggtgcgca gctgcaggat
     9301 gagatccgga tggaagcggc tgcggggcag ggccacgttg gagaattcca gcgtgtcggc
     9361 catccgcccg acccggtcca cctgacgcac cagcaggtat ttgcgcttga ccgtggcccg
     9421 gtccatgttc ttggagctgc cgaaggtgtc cttgatgatc ttgaagacgt aggggtagga
     9481 cggcagcgtg aacaccagca tcaccagccc gcggatgccc ggtgcctcga tgaactgatc
     9541 gtttgaatgg cgcaggtgcg acaccaggtc gcggaagaag ctggtcttgc cctgcttgcc
     9601 caggcccagc atggtgtaga gctccgagcg cggcttgttg ggcatgatgc tgcgcaggaa
     9661 ctgcacgtag cctgagggga cctccatgtc caccaggaag taggcccgcg acagggagaa
     9721 cagcagggag atgcgccagg tgtcgagcag gatggtgtcc aggtagagca tgcccgaggc
     9781 accgtggcgc acggcgaggg cgaaggggat ttcctggtag ccgttgaggc ccttgccgat
     9841 gatgtaggcg gtcttgttgc ggtagaaggc ggagtacagc acctggatct ggcaattcac
     9901 ctccatcctg ggccagccgc ccagatgggt ctcgaccgtg cgcagcacgt aatccacgtc
     9961 ccgctccaga tcctcgaagc tgcgctgcca gtcgaaatcc tcgacgatgc gccgcacggt
    10021 ggcgcgcagg ccttcgtcct gggggtagta gctggaatag gtgggcggga aggactcgat
    10081 gtaatccgtc gagatcgccg ggcgggtgaa gatgtagtcg ttgctgaagt aatcccggtg
    10141 caggatcttg gtggtgacgg agttgaagaa ggtttccgcc agctccggct gcttgtggtt
    10201 gaggagcagg ccgatgtagt gcagcttgac ggcctgccag gtggcgtcat cgatggattc
    10261 cgcgtcgaat tcctcgtgca ggcgctgcac ggtctcgttg acccggtcat cgtagaactg
    10321 cacccggttg cgcaccgccg agagctgatc ctgccagtcg gcggcctcga agctctcctt
    10381 tgcatggcgc gaggtctccc ggaagatccg gtagtgcttg ttgaagccct cgatcatcgc
    10441 ctgggcgatg gcctgggcga cggggttttc gttgccgtgg gtgtccattc agcctccgca
    10501 cactctccat tcggcccgtt cgcgcgaggg cgcgggtaca tcgccgcgac ggggtcgagg
    10561 ctgcatctta gcatcgcccg acgggccttc cggaagggag gcgaaacgcc cggatcgcgc
    10621 tgcacagcag catttccggc gctttgtggc gtcatgcatt tcagccataa tcgtcgccgc
    10681 cagaccctgc ctgtccacac caccgggcgg gtcggcagtg ctgcgatcgc gccggtgcgg
    10741 gccgcgttac gcgtgtcacc tcgggcttgc ccccggtatc ggccgatctg cctttccatc
    10801 gaattacgag cgagggaact ggaatgagta cttcactgat caaggtgcct gccggcggcc
    10861 aaaaaatcgt cccgggccag ccgacgccgg acaatcccat catcccctac atcgaaggtg
    10921 acggcatcgg ggtggacatc accccggtga tgatccaggt catcgatgcc gcagtcacca
    10981 aggcctacgg cggccagcgc aagatccact ggatggaggt ctacgccggc gaaaaatcca
    11041 cccagctgta tggtcccgat gaatggttcc cgaaggagac cttcgacgcc ctcaaggaat
    11101 tctccgtctc catcaagggc cccatgacca cgccggtggg cggcggcatc cgctccctga
    11161 acgtggccct gcgccaggag ctcgatctct accagtgcgt gcggccggtg cgttacttca
    11221 agggcgtgcc cagcccgctc aagcagcccg agctgaccga catggtcatc ttccgcgaga
    11281 acaccgagga catctacgcc ggcatcgagt gggcggcaga gtcggacaac gtgaagaagg
    11341 tcatcgcctt cctgcagcag gagatgggcg tcaagaagat ccgctttccg gaaagctccg
    11401 gcatcggcat caagcccgtg tccatcgaag gcaccacgcg ccttgttcgc gccgccatca
    11461 attacgccat cgccaacgac cgcaagagcg tcaccctggt gcacaagggc aacatcatga
    11521 agttcaccga gggcctgttc cgcgacaccg gctacaaggt ggcgcagcag gaattcggcg
    11581 cccagcccat cgacggcggc ccgtggtgtt ccttcgtcaa ccccaggacc ggccgcacga
    11641 tcatcgtcaa ggacgccatt gccgacgcct tcctgcagca gatcctgctg cgcccggccg
    11701 aatacgacgt gatcgccacc accaacctca acggcgacta catctcggac gccctggcgg
    11761 cccaggtcgg cggcatcggc atcgcgccgg gcgccaacat ctctgaccag tacgcctgct
    11821 ttgaggccac ccacggcacc gcgcccaagt acgccggcct cgacaaggtg aatcccggct
    11881 cgctgatcct ctccgccgag atgatgctgc gccacctggg ctgggtggag gcggccgatc
    11941 tggtcatcaa ggccatggaa gccgccatcg gcgacaagca ggtcacctac gacttcgcgc
    12001 gtctgatgga gggcgccacc gaagtgtcct gctccggctt cggcgaggcg atggtcgcgc
    12061 gcatgtaaca cctcagcccc gcgccgatcc cggcacgaaa acgcccccgc atgtcggggg
    12121 cgttttc
//
LOCUS       DAOQPA010000106        11900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2761_length_11900_cov_16.258675, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000106 DAOQPA010000000
VERSION     DAOQPA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_11900_cov_16.258675"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKX01_13425"
     CDS             <1..252
                     /locus_tag="PKX01_13425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ77178.1"
                     /translation="GRLLPDGHVGRMAVLPAWRGRGVGRAVLTALMAEARRRGLSRLV
                     LHAQTHAEGFYAGFGFVAEGEVFDDAGLPHRTMVWQVQG"
     gene            complement(336..1010)
                     /locus_tag="PKX01_13430"
     CDS             complement(336..1010)
                     /locus_tag="PKX01_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ77179.1"
                     /translation="MKLRHTPLSIAAGPVWLDGLLAHAPDARGLVVIAQNAAGHHQDS
                     REAHFAARIEAAGFATLIFDLLTRYEETRDPDTRYNAPLLGQRLNALFEWIRHQPPLE
                     GLAVGLVTSGNGCAAAIRAMSREPEFIAALVCRGGRPGLAGADPLRLVQSPACMIVGE
                     RDEGIAGVRQAYELLGGPKAWREIPGTDGLFREPGTLDEAASHAADWFLRHLPPPAGQ
                     APAMTA"
     gene            complement(1075..2184)
                     /gene="amrS"
                     /locus_tag="PKX01_13435"
     CDS             complement(1075..2184)
                     /gene="amrS"
                     /locus_tag="PKX01_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HMZ77180.1"
                     /translation="MEGIGGPDALNGVPARYWHRLDDGRIQCDLCPRDCRLHAGQRGA
                     CFVRQRVGDGLVLTTYGRSSGFCIDPIEKKPLNHFHPGSSVFSFGTAGCNLACKFCQN
                     WDISKSRDMDRLMDAAAPDEIAATAAAHRCRSVAFTYNDPVIFAEYAIDTAVACHARG
                     LATVAVTAGYISPAPRRDFFAVMDAANVDLKAFTDDFYVHQTGARLAPVLDTLLYLRH
                     ETDVWVEITTLLIPGLNDSDAELSALSRWVADELGTDVPLHFTAFHPDYKLDTLPPTP
                     PATLRRARRIALDAGLAYVYTGNVRDIEGASTRCPACGQTLIERDGYRILAYRLDAHG
                     ACTACGHVIAGHFGDFTGAFGPRRVPVHIHRHAGP"
     gene            complement(2154..2720)
                     /gene="amrA"
                     /locus_tag="PKX01_13440"
     CDS             complement(2154..2720)
                     /gene="amrA"
                     /locus_tag="PKX01_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HMZ77181.1"
                     /translation="MPATDALGPVLLAHARAAIADALGLPHAPAPEDPRLSERRASFV
                     TLTRNGELRGCIGSLSAHRPLGEDVRANAAAAALKDSRFPPLDAREFDAVRVEVSVLT
                     APEFLEFRDEADALSQLVPGRDGVIFFNGCQKATFLPQVWAQLPEPRAFLAALKQKAR
                     LPVDFWGPNVMIARYEVEKWKESEVPTP"
     gene            complement(2707..3525)
                     /gene="amrB"
                     /locus_tag="PKX01_13445"
     CDS             complement(2707..3525)
                     /gene="amrB"
                     /locus_tag="PKX01_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HMZ77182.1"
                     /translation="MAHASQRPPAVAGLFYPADPHSLHAQISGLLAGAVPAERIDCPK
                     ALVVPHAGYIYSGAVAATAYEALARRRGVLRRVVILCPTHRVAIRGMALPASLSFITP
                     LGAVPVDQDAWRLAQTLPDVVVDDRPHAQEHAIEVQLPFLQCVLDHFAIVPIAVGQAD
                     AASVAAAIEALWGGPETLILVSSDLSHYHPYRQAQLRDKATLAQIQQLDPRIEPEQAC
                     GAGPINGLLLAALRHHLRPQVLDQRNSGDTAGDRNRVVGYAAIAFSEDLDHACH"
     gene            complement(3567..4844)
                     /locus_tag="PKX01_13450"
     CDS             complement(3567..4844)
                     /locus_tag="PKX01_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMZ77183.1"
                     /translation="MNTLLFHQAFDPAQLEALAVATAIGLLMGLERERHPASRAGVRT
                     FGLTGLLGALCALLAERLGTPGVLVAGLVLVAVMMIAAYLRRPDPHDPGTTSVIAILV
                     CFALGAMVWLGFGRIAVMAAVGATMLLYFKSELSGVAARLTAQDWRSILQFAVLSLIV
                     LPILPDRGFGPYEALNPHQIWLMVVLIAGVSLAGYAALRLAGARLGAPLLGLLGGLVS
                     STATTMVFSRHARENPAMVSTAKLVILLANLVMVVRVGGIALAVAPSVAPRLWPGAGL
                     ALLAGAAVLAWNWRQLVHQGELPIPDTRNPTELRAAMGFGLLYALVLLCAAWLSDIAG
                     QGGLYMLALASGLTDVDAITLSMLRLLNTGKLGADPAATGILLAMLANLSFKSGLALS
                     LGGRHLGSGVAAGMAAVGAGLGGGIAWLHLASL"
     gene            4916..5623
                     /gene="ispD"
                     /locus_tag="PKX01_13455"
     CDS             4916..5623
                     /gene="ispD"
                     /locus_tag="PKX01_13455"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HMZ77184.1"
                     /translation="MHTHHSRCFALVPAAGSGSRMKSEQPKQYLSLLGEPLIRHTLAV
                     LCASPLLERVYVVLSVGDQEWDRHPWHSFGDKLVPLFCGGASRADSVLNGLRAIADDT
                     ASSDWILVHDAARPCLAPWHIESLVRELIHDEVGGILAVPVADTLKRADSQQRIAATV
                     PRDGLWQAQTPQMFRYVMLKRALEAARDVTDEASAIEAMGLRPRLVRGDPTNLKVTYP
                     LDLHLAEWILQHREGLA"
     gene            5620..6111
                     /gene="ispF"
                     /locus_tag="PKX01_13460"
     CDS             5620..6111
                     /gene="ispF"
                     /locus_tag="PKX01_13460"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HMZ77185.1"
                     /translation="MIVPFRIGQGFDVHALVPGRALILGGVTIPHTHGLLGHSDADVL
                     LHALTDAILGAAGLGDIGRHFPDTDPRFEGADSRVLLREAAAAVRRAGWQTVNVDATV
                     IARAPKILPHVPAMVACIAADLEVAPEAVNIKGKTTEKLGFTGRGEGIAAQAVALLAR
                     VET"
     gene            complement(6310..8310)
                     /locus_tag="PKX01_13465"
     CDS             complement(6310..8310)
                     /locus_tag="PKX01_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMZ77186.1"
                     /translation="MPFSERGPLVPRISDDDIVASAWESFVRNAAVSGPGLRSVVLDS
                     WQRSRAGAVDPGRHSAPGADMERVRRLLWQNRELCEVARPVLEGLREVLRECGSLIML
                     SDPAGTILDLNGGTRARSVGEEINLATGGCWSEALIGTNAIGTAIATRAPVQIYANEH
                     FCVDVKRWTCAAAPILDRSGRVRGVVDVSGVKETFHGHTLGLVMAAARQIEADFAHRD
                     EVLNSRLLVAAITDFSRYSSDCLLLVDHRGRILRVAGDLGAVRTRLHLPDRLDVGSVL
                     AALELPQDGESRALHLPGWVQPEWLRPIKDGPLRLGTLIVIPTVAGRTSPARASSATS
                     PADLGTDAFADIIGNSEVLQIAKNRARRIAPLDLPVLLLGETGAGKELFARALHRAGN
                     RPEGPFVAVNCGALTRELLASELFGYAEGAFTGARRGGHPGKFEQAHGGTLFLDEIGE
                     MPIDMQPYLLRAIQDGVIVRVGDTRERRVSVRLVAATNRDLPDAIAAGRFREDLYHRL
                     GVVTLNLPALRDRPGDIDSIVDHLNRRLADKYGGTPKVLTPAVRAALQAYRWPGNIRE
                     LQNVIEVMFALCEGDTIDVPLLPQTLTRSEATRPLAARPAAVTAPVPAGRLDDVQRQA
                     IQAAVANARGNLSLAARTLGISRSTLYVKLASMRPADETSVR"
     gene            8654..10798
                     /locus_tag="PKX01_13470"
     CDS             8654..10798
                     /locus_tag="PKX01_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HMZ77187.1"
                     /translation="MQQRSTVQVMGIDAGGTMTDTFFVRDDGRFVVGKAQSNPGDESL
                     AIYNSSVDALAHWNRTVDDVYPELVTCVYSGTAMLNRILMRKGLDVGLICNKGFEQIH
                     SMGRALQSYLGYALEDRIHLNTHRYDEPLVPVSRTRGVTERTDVQGRVVIPLREDEVR
                     AATRELVEAGAQAIVICLLQSHKNEASEQRARDLVREELARLGVELPVFASVDYYPSR
                     KESHRMNTTILEAYGAEPSRQTLRKVSDRFKKHGARFDLRVMATHGGTISWKAKELAR
                     TIVSGPIGGVIGSRLLGEALGDENIACSDIGGTSFDVALITKGNFAIKSDPDMARLVL
                     SLPLVAMDSVGAGAGSFVRLDPYSKSIKLGPDSAGYRVGTCWPESGLDTVSVSDCHVV
                     LGYLNPNNFLGGAIKLDVERARQHIKAQIADPLGLSVEDAAAGVIELLDLTLSEYLRA
                     NISAKGYNPADFTCFSYGGAGPVHTYGYTEGVGFKDVVVPAWAAGFSAFGCACADFEY
                     RYDKSVDLGVAQHAADTVKSAACRTLQDAWSELAVKVIDEFVLNGYKAEDVILIPGYK
                     MQYMGQLNDLEIVSPVSSASSAADWDRIVDAFETTYGRVYASSARSPELGFSITGAIL
                     RGMVVTQKPVLPEDADGGPTPPKEAYLGTRPFYRHKKWVDAALWKMEALKAGNHIVGP
                     AIIESDATTFVVPDGFETTIDKHRLFHLKEVL"
     gene            10847..>11900
                     /locus_tag="PKX01_13475"
     CDS             10847..>11900
                     /locus_tag="PKX01_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HMZ77188.1"
                     /translation="MNMLSKKEVGFADLLKNGQTLKEFRDGIIERTQASGHYNGLERL
                     EFRDRDPIGYEKLFSKLRGGLVHARETAKKIAASPIVEQEGELCFTLYNAVGDCVLTS
                     TGIIIHVGTMGAAIKYMIENNWEANPGIRPGDMFTNNDCSIGNVHPCDIATIVPIFWE
                     GRLIGWVGGVTHVIDTGSVTPGSMSTGQTQRFGDGYMITCRKTGINDEPLRDWLHESQ
                     RSVRTPKYWILDERTRIAGCHMIRDLVEEVIRADGIEAYEKFAYEVIEEGRRGLMSRI
                     KAMTLPGKYRKVSFVDVPYKHEDVQVSNAFAKLDSIMHSPCEMTIRPDGKWRLDFEGA
                     SRWGWHTFNAHQVAFTS"
BASE COUNT         1957 a         3881 c         4263 g         1799 t
ORIGIN      
        1 ggtcgcctgt tgccggacgg gcacgtggga cgcatggcgg tgctgccggc gtggcgcgga
       61 cggggcgttg gccgtgccgt gctgacggcg ttgatggccg aagcccgccg gcgcggtctg
      121 tcacggctgg tgttgcacgc ccagactcac gcagaaggct tctacgcggg cttcggcttc
      181 gtggcggagg gcgaggtctt cgacgatgcg ggtctgcccc atcggaccat ggtgtggcag
      241 gtgcaggggt gatcccttcg cgcttcaggc aacgggcctg cgcgggtcgc ccaccccgtc
      301 ccggagtgcc ttgagcacag tgtccgaagg cgccgtcagg cggtcatggc gggtgcctgc
      361 ccggcggggg gcggcaggtg gcgcaggaac cagtcggcgg cgtggctggc ggcctcgtcg
      421 agggtgcccg gttcgcggaa caggccgtcg gtgccgggta tctcgcgcca ggccttgggg
      481 ccgcccagca gttcataggc ctgccgcacg ccggcgatgc cttcgtcccg ctcgccgacg
      541 atcatgcagg cagggctctg caccaggcgc agggggtcgg cgcccgccag tccggggcgt
      601 ccgccgcggc acaccagggc ggcgatgaat tccggctcgc ggctcatggc gcggatggcg
      661 gcggcgcagc cgttgccgct ggtgaccagc ccgacggcga gtccttccag cggaggctga
      721 tggcggatcc attcgaagag ggcgttgagg cgttggccca gcaggggggc gttgtaacgg
      781 gtgtccggat cgcgggtctc ctcgtagcgg gtgaggaggt cgaagatcag ggtggcaaag
      841 ccggcggctt cgatgcgggc ggcgaagtgc gcctcgcggg agtcctgatg atggccggcg
      901 gcgttctggg cgatcaccac caggccgcgg gcatccgggg catgggcgag cagcccgtcg
      961 agccagacgg ggccggcggc gatgctgagg ggcgtgtggc ggagtttcat ggtcggattc
     1021 atcgcttgca cgatggcacg ggccggcagg catcgcaagc cggcggcgcc cgcttcaggg
     1081 gcctgcgtgc cggtggatgt gcactgggac acggcgcggc ccgaaggcgc cggtgaagtc
     1141 gccgaaatgc ccggcgatca cgtgtccgca ggccgtgcag gcaccatggg catcaaggcg
     1201 gtaggcgagg atgcggtagc cgtcccgctc gatgagcgtc tgtccgcagg cggggcagcg
     1261 ggtcgaagcg ccttcaatgt cgcgtacgtt gcccgtgtag acgtaggcca ggcccgcgtc
     1321 cagcgcgatg cggcgggcgc ggcgcagggt ggcgggcggg gtcggcggca gggtgtcgag
     1381 cttgtagtcg gggtggaagg cggtgaagtg cagcggcacg tcggtcccca gttcgtcggc
     1441 cacccagcgg gacagggcgc tcagctcggc gtcggaatcg ttgaggccgg ggatcagcag
     1501 ggtggtgatc tcgacccaca cgtccgtctc gtggcgcaga tagaggaggg tgtcgaggac
     1561 cggggcgagg cgggcgccgg tctggtgcac gtagaagtcg tcggtgaagg ccttgagatc
     1621 cacattggcg gcgtccatca cggcgaagaa gtcgcgccgc ggcgcaggtg agatgtagcc
     1681 ggcggtgacg gccaccgtgg cgaggccgcg ggcgtgacag gcgacggcgg tgtcgatggc
     1741 gtactcggcg aagatcaccg ggtcgttgta ggtgaaggcg acgctgcggc agcggtgggc
     1801 tgcggcggtg gcggcgatct cgtccggcgc ggcggcgtcc atcaggcggt ccatgtcccg
     1861 gctcttggaa atgtcccagt tctggcagaa cttgcaggcc aggttgcagc cggcggtgcc
     1921 gaaggagaag acgctgctgc cagggtggaa gtggttgagg ggcttcttct cgatggggtc
     1981 gatgcagaag cccgaactgc gcccgtaggt ggtgagcacc aggccgtcgc ccacgcgctg
     2041 gcgcacgaag caggcgccgc gctggcctgc gtgcaggcgg caatcccgcg ggcacaggtc
     2101 gcactggatg cggccgtcgt cgagccggtg ccagtagcgg gcggggacgc cgttcagggc
     2161 gtcgggacct ccgattcctt ccatttttcc acctcgtagc gggcgatcat cacgttcggc
     2221 ccccagaaat ccaccggcag gcgggccttc tgcttgaggg cggcgaggaa ggcgcggggc
     2281 tccggcagct gcgcccagac ctggggcagg aaggtggcct tctggcagcc attgaagaag
     2341 atcaccccgt cgcggccggg gacgagctgg gacagggcgt ctgcctcgtc gcggaattcc
     2401 aggaattccg gcgcggtgag gacggacact tccacccgga cggcgtcgaa ctcgcgggcg
     2461 tccaggggcg gaaagcggga atccttgagg gccgccgctg ccgcgttggc gcgcacatcc
     2521 tcacccagag ggcggtgggc gctgaggctg ccaatgcagc cgcgcagctc gccgttgcgg
     2581 gtcagcgtga caaagctggc acgtcgctcg gacaggcgcg gatcttctgg tgcgggggca
     2641 tggggcaggc caagggcgtc ggcaatggcg gcacgggcgt gggccagcag cacggggccg
     2701 agggcgtcag tggcaggcat ggtccagatc ctcggaaaag gcgatggccg cgtagccaac
     2761 cacccggttg cggtcgcccg cggtgtcgcc ggagttgcgc tgatccagca cctggggacg
     2821 caggtgatgg cgcagggctg cgagcagcag gccgttgatc ggcccggcgc cgcaggcctg
     2881 ctcgggctcg atgcgcgggt cgagctgctg gatctgggcc agggtggcct tgtcgcgcag
     2941 ctgggcctgg cggtaggggt ggtagtggga gagatcggaa ctgacgagga tcagtgtctc
     3001 cgggccgccc cacagggcct cgatggcggc cgccacggag gcggcatcgg cctgacccac
     3061 cgcgatgggc acgatggcga aatgatccag cacgcattgc aggaagggca gctgcacctc
     3121 gatggcatgc tcctgggcgt gggggcggtc atctacgacc acgtcgggca gcgtctgggc
     3181 gaggcgccag gcgtcctgat ccacggggac cgcgcccaag ggtgtgatga aggacaggct
     3241 ggcgggcagc gccatgccgc ggatcgcaac acggtgggtc ggacagagga tcacgacgcg
     3301 gcgcaggacg ccacggcggc gggcaagcgc ttcataggcg gtggccgcca ccgcaccgga
     3361 atagatataa cccgcgtggg gcacgacaag ggccttggga cagtcgatgc gctcggccgg
     3421 gaccgcgccg gccagcaggc cactgatctg ggcatgcagg ctgtgggggt cggccggata
     3481 gaacagcccg gcgaccgccg gcggacgttg ggatgcgtgt gccatgatgc ctttctcccg
     3541 gtgaggggac cgaatgtcac ttcgtattat agggacgcca gatgaagcca cgcgatgccg
     3601 ccaccgagtc ccgcgccgac ggccgccatg ccggccgcca cgccgctgcc caggtgccgt
     3661 ccgcccaggc tcagggcgag gccgctcttg aagctcaggt tcgccagcat ggccaacagg
     3721 atgccggtgg cggcggggtc ggcgcccagc ttgccggtgt tgagcaggcg cagcatcgaa
     3781 agggtgatgg catccacgtc ggtgagtccg gaggccaggg ccagcatgta gaggccgccc
     3841 tggccggcga tgtccgacag ccaggccgcg cacaacagga ccagggcgta gagcaggccg
     3901 aagcccatgg cggcccgcag ttccgtcggg ttgcgggtgt ccgggatggg cagttcgccc
     3961 tgatggacga gttgacgcca gttccaggcc agcacggcgg cacctgccag gagcgccaga
     4021 ccggcacccg gccacaggcg cggcgccacc gacggggcca cggcgagggc gatgcccccc
     4081 acgcgtacga ccattaccag attggcgagc aggatcacca gtttggccgt ggacaccatg
     4141 gccggattct cgcgggcatg gcgggagaac accatggttg tcgcggtgct cgacaccagt
     4201 ccgccgagca ggcccagcag cggcgcaccg aggcgcgcgc cggccagacg cagggcggcg
     4261 tagccggcca gactcacgcc ggcgatgagc acgaccatca accagatctg gtgcgggttg
     4321 agggcctcgt aggggccgaa gccccggtcc ggcaggatcg gcaggacgat cagcgagagc
     4381 accgcgaatt gcaggatcga acgccaatcc tgggctgtga ggcgggccgc cacgccgctc
     4441 agttctgatt tgaagtagag cagcatcgtc gcgccaaccg cggccatcac cgcaatgcga
     4501 ccgaagccca gccacaccat tgcgcccagg gcgaagcaca ccaggatggc gatgaccgag
     4561 gtggtaccgg gatcgtgggg gtcgggcctg cgcagatagg cggcgatcat catcaccgcc
     4621 accaggacca gacccgccac cagcacgccc ggcgtgccga gccgctccgc cagcagggcg
     4681 cagagggcgc cgagcagtcc ggtgagcccg aatgtgcgca cgccggcgcg ggaggcggga
     4741 tggcgctccc gctccaggcc catgagcaga ccgatggcgg tggcgaccgc gagggcttcc
     4801 agctgggcgg gatcgaaggc ctgatggaag agcagggtgt tcatggcgtc agcgccggag
     4861 gggccccgcg ccccgaaaac ggcggccgac ggggacattg agtagaatcc gccccatgca
     4921 cacccatcac agccgatgtt ttgccctcgt gcctgccgcc ggaagcggca gccgcatgaa
     4981 gagcgaacag cccaaacaat atctttccct cctcggcgaa cccctgatcc gccacaccct
     5041 ggcggtattg tgcgcctctc cgctgctgga gcgggtgtac gtggtgctgt cggtgggcga
     5101 tcaggagtgg gaccgccacc cctggcattc gttcggcgac aagctggtgc cgctcttttg
     5161 tggtggggcc tcgcgggccg acagcgtcct caacggcctg cgcgccatcg ccgacgacac
     5221 cgcatcctcg gactggatcc tcgtccacga tgcggcgcgg ccctgcctcg cgccgtggca
     5281 cattgaaagc ctggtgcgtg agctgatcca cgacgaagtg ggcggcattc ttgctgtacc
     5341 ggtggccgac accctcaagc gcgccgacag ccagcagcgc atcgccgcca cggtgccgcg
     5401 ggacggcctg tggcaggccc agacgcccca gatgttccgc tacgtcatgc tcaagcgtgc
     5461 ccttgaagcc gcccgcgacg tgaccgacga ggccagcgcc atcgaggcca tgggtctgcg
     5521 gccgcggctc gtgcgtggcg atcccaccaa tctcaaagtg acctatcccc tcgacctgca
     5581 tctggccgag tggatcctgc aacatcgcga aggactcgca tgatcgttcc gttccgcatc
     5641 ggccagggct tcgacgtgca cgccctggtg cccggccgcg ccctcattct cggcggcgtc
     5701 accattcccc acacccacgg cctgctgggt cactcggacg ccgacgtgct gctgcacgcc
     5761 ctcaccgacg cgatcctcgg cgcggcgggg ctgggagaca tcggccgcca tttccccgac
     5821 accgacccgc gcttcgaggg cgccgattcc cgcgtgctgc tgcgcgaggc cgccgccgcg
     5881 gtgcgccgcg ccggctggca gacggtgaat gtggacgcca ccgtgattgc ccgcgcgccg
     5941 aagatcctgc cccacgtgcc ggccatggtg gcgtgtatcg ccgccgacct ggaggtggcg
     6001 ccggaggccg tcaatatcaa gggcaagacc accgagaagc tgggcttcac cggtcgcggt
     6061 gagggtatcg cggcccaggc ggtcgcgctg ctggcgcgcg tggagacctg aggcgcggac
     6121 ggcgcaaccc tgacgacgcg ccggggagcg tcggcatcgc gcgctgtggc gcggcgccgc
     6181 gcgcccatcg cgggcggtga ttccgggagc cgatgatgga ccgggccgca gatcggggcg
     6241 tgaccgtgcc ttcaggcccg cctccggtcc tcagctaggg ggggggccgt gtggtccgtc
     6301 gcgctgccgt cagcgcacag aggtctcgtc cgcggggcgc atgcttgcca gcttgacata
     6361 cagggtgctg cgggagattc ccagggtccg cgcggccagg gacaggttgc cccgcgcgtt
     6421 ggcgaccgca gcctggatcg cctggcgttg cacgtcgtcg aggcggccgg cgggcacggg
     6481 ggcggttacg gcggccggac gggcggccaa tgggcgggtt gcctcgctgc gggtgagtgt
     6541 ctggggcagc agcgggacat cgatcgtgtc gccctcacag agcgcgaaca tcacctcgat
     6601 cacgttctgc aactcgcgga tgttgccggg ccagcggtag gcctgcaacg ctgcacgcac
     6661 cgcgggcgtg agcactttgg gcgtaccccc gtatttgtcg gcaaggcgcc ggttgaggtg
     6721 gtcgacgatg ctgtcgatgt cgcccgggcg atcgcgcagc gcgggtagat tcagggtgac
     6781 gacgcccagg cggtgataca gatcctcccg gaaacgcccc gcggcgatgg cgtcgggcag
     6841 atcccggttg gtggcggcca ccagccgcac cgagacgcgc cgctcacgcg tgtcaccgac
     6901 ccgcacgatc accccgtcct ggatcgcccg cagcaggtac ggctgcatgt cgatcggcat
     6961 ttcaccgatt tcatcgagaa acagcgtccc gccgtgggcc tgttcgaact tgcccggatg
     7021 ccccccgcga cgggccccgg tgaaggcgcc ttcggcgtag ccgaacagct cgctggccag
     7081 cagctcccgc gtgagggcgc cacagttcac tgccacgaac gggccctccg ggcgatttcc
     7141 cgccctgtgc agggcgcggg caaacagttc cttgccggca ccggtctcgc ccagcagcag
     7201 caccggcagg tccagcgggg caatacgccg ggcccggttc ttggcgatct gcagaacctc
     7261 gctgttgccg atgatgtcgg cgaaggcgtc cgtgccgagg tcggcgggtg atgtcgcgct
     7321 gctggcgcgt gcgggcgatg tccgcccggc gacggtcggg atcacgatga gggttcccag
     7381 gcgcagcggc ccgtccttga tcgggcggag ccattcgggc tgcacccagc caggcaggtg
     7441 gagggcgcgt gactcgccgt cctggggcag ttcgagggcg gcgagcacgc tgcccacgtc
     7501 caggcggtcc ggcaggtgga ggcgggtacg gactgcgccc agatcccctg caacgcgcag
     7561 gatgcgtccc cggtgatcca ccagcagcag gcagtcactg ctgtaacggg aaaaatcggt
     7621 tatggcggcg accagcaggc ggctgttgag cacttcgtcc cggtgggcga agtcggcttc
     7681 gatctgccgc gcggcagcca tgaccagccc cagggtgtgg ccgtgaaagg tctctttcac
     7741 gcctgacaca tccaccacac cgcgcacccg gccgctgcgg tcgagaatcg gcgcggcggc
     7801 acaggtccag cgcttcacgt ccacgcagaa atgttcgttg gcgtagatct ggaccggtgc
     7861 ccgcgtggcg atggccgttc cgatggcgtt ggtgccgatc agcgcctcgc tccagcagcc
     7921 gccggtggcg aggttgatct cctcgcccac gctgcgggca cgggtgccac cgttcaggtc
     7981 gaggatcgtg ccagccggat cgctcagcat gatgaggctg ccacattcgc gcagcacctc
     8041 gcgcaggcct tccagcaccg ggcgggccac ttcacacagc tcacggttct gccacagcag
     8101 acggcgcacc cgttccatgt cggcgcccgg tgccgaatgg cggcccggat cgacggcccc
     8161 ggcccggctg cgttgccacg agtcgagcac cacgctgcgc agtcccggtc ccgatacggc
     8221 ggcgttccgc acgaaggatt cccacgccga cgcgacgatg tcgtcatcgc tgatgcgtgg
     8281 aacgaggggg ccgcgctcgg aaaagggcat ggggcgggga acaggcgtcc gggaaaaggt
     8341 cgcgaccacc gtagcgtcaa gcgcggacaa agtcaataaa gtccttaaag atcaatgatt
     8401 tgtgcgttgc gcaaaagtgt ccggattccg gacaggtgag ggccggcgtc aggaatccgg
     8461 acatcgcatc ggacagaccg gcgacgccgg aggacggtcg gacgaagcgc gccgcgtccc
     8521 ctgctgattt gttcatctga ttgaatcata aggacagttt ctgcgtgggc cgcatgcggt
     8581 ccggtgtctg gcccggcctt tgcaccgggg tgtccgtggg gcaagcccac tcacaccaac
     8641 gacaggaggc aacatgcaac aacgatccac cgtccaggtc atgggcatcg atgccggcgg
     8701 cacgatgacc gataccttct ttgtccgcga cgacggccgc ttcgtggtcg gcaaggccca
     8761 gagcaatccg ggcgatgaat ccctcgccat ctacaactcg tcggtagacg cgctggcgca
     8821 ctggaaccgt acggtcgacg acgtctaccc ggaactggtg acctgtgtgt actcgggtac
     8881 tgcgatgctc aaccgcatcc tgatgcgcaa ggggctcgat gtcggcctga tctgcaacaa
     8941 gggcttcgaa cagatccact cgatgggtcg cgccctgcag agctacctgg gctacgccct
     9001 ggaagaccgc atccacctca acacgcaccg ttacgacgag ccgttggtcc cggtctcgcg
     9061 cacccgcggc gtgaccgagc gtaccgacgt gcaggggcgc gtggtcattc ccctgcgcga
     9121 ggacgaggtc cgcgcagcca cccgggagct ggtcgaggct ggcgcccagg ccatcgtcat
     9181 ctgcctgctc cagtcccaca agaacgaggc cagcgagcag cgtgcccggg atctcgtgcg
     9241 ggaagagctg gcccggctgg gggtggagct cccggtgttc gcgtcggtgg attactaccc
     9301 ctcgcgcaag gaaagccacc ggatgaacac caccatcctg gaggcctacg gcgcggagcc
     9361 gtcgcgccag accctcagga aggtgagcga ccgcttcaag aagcacggtg cccggttcga
     9421 tctgcgggtc atggccaccc acggcggcac gatcagctgg aaagccaagg agctcgcccg
     9481 caccatcgtt tccggtccca tcggcggcgt gatcggctcc cgcctgctgg gcgaagcgct
     9541 gggcgacgag aacattgcct gctcggacat cggcggaacc agcttcgacg tggcgctgat
     9601 caccaagggc aacttcgcca tcaagagcga tcccgacatg gcccgcctgg tcctgtcgct
     9661 gcccctcgtg gccatggatt ccgtgggcgc cggcgcgggc agcttcgtgc gcctcgatcc
     9721 ctacagcaaa tcgatcaagc tggggccgga ttcggcgggc tatcgggtgg gcacgtgctg
     9781 gccggaaagc ggcctcgaca cggtgtcggt ctccgactgc cacgtggtgc tgggctacct
     9841 gaaccccaac aacttcctcg gcggcgccat caagctggac gtggagcgcg cccgtcagca
     9901 catcaaggca cagattgccg atccgctggg cctctcggtt gaggacgcag ccgccggggt
     9961 catcgaactg ctcgacctga cgctctcgga atatctgcgc gccaacatca gcgccaaggg
    10021 gtacaaccct gccgacttca cctgcttctc ctacggcggc gcggggccgg tccataccta
    10081 tggctacacc gagggtgtgg gcttcaagga tgtggtggtg ccggcctggg cggcaggttt
    10141 ctccgcgttc gggtgcgcct gtgccgattt tgaataccgc tacgacaagt cggtggatct
    10201 gggcgtcgcc cagcacgcgg ccgacaccgt gaagtccgcc gcctgccgca ccctgcagga
    10261 cgcctggtcc gagctggcgg tcaaggtcat cgacgagttc gtcctcaacg gctacaaggc
    10321 cgaggacgtg atcctgatcc ccggctacaa gatgcagtac atgggccagc tgaatgacct
    10381 ggagatcgtc tctccggtgt cctcggcatc ctcggcggcg gactgggacc gcatcgtcga
    10441 cgccttcgag accacctatg gccgggtcta tgccagctcg gcccgctcgc ccgagctggg
    10501 cttctcgatc accggcgcga tcctgcgcgg catggtggtg acccagaagc cggtgctgcc
    10561 ggaggacgcg gacggcggtc cgacgccgcc gaaggaggct tacctgggca cccgcccgtt
    10621 ctaccgccac aagaagtggg tggacgccgc cctgtggaag atggaagcgc tcaaggccgg
    10681 caaccacatc gtcgggccgg ccatcatcga atcggacgcc accacctttg tcgtgccgga
    10741 cggcttcgag accaccatcg acaagcaccg cctgttccac ctgaaggaag tcctctgaca
    10801 ccgcgcagct gcccgggtgg accacaacga tctaggagac aaggtaatga atatgttgag
    10861 caagaaggaa gtcggcttcg ccgatctgct gaagaacggc cagaccctga aggaattccg
    10921 cgacgggatc atcgagcgga cccaggccag cgggcactac aacggtctcg agcgcctgga
    10981 attccgggac cgcgacccga tcggctacga gaagctgttc tcgaaactgc gcggcggcct
    11041 ggtgcatgcc cgcgaaacgg ccaagaagat cgccgccagc cccatcgtcg agcaggaagg
    11101 ggagctgtgc ttcacgttgt acaacgcggt cggcgactgt gtgctcacct ccaccggcat
    11161 catcatccac gtggggacga tgggggcggc gatcaaatac atgatcgaga acaactggga
    11221 ggccaacccc ggcatccgcc ccggtgacat gttcaccaac aacgactgct ccatcggcaa
    11281 cgtgcatccc tgcgacatcg ccaccatcgt gccgatcttc tgggaaggcc ggctgatcgg
    11341 ctgggtgggc ggcgtcaccc acgtgatcga caccggctcg gtcacgcccg gctccatgtc
    11401 caccggtcag acccagcgct tcggtgacgg ttacatgatc acctgccgca agaccgggat
    11461 caacgacgaa cccctgcgtg actggctgca tgaatcccag cgctcggtgc gcacgcccaa
    11521 gtactggatc ctcgacgagc gcacgcgcat cgccggctgt cacatgatcc gtgacctggt
    11581 ggaagaggtg atccgcgccg acggcatcga ggcctacgag aagttcgcct acgaagtcat
    11641 cgaagaaggg cgccgtggcc tgatgagccg catcaaggcc atgaccctgc cgggcaagta
    11701 tcgcaaggtc tccttcgtgg atgtgccgta caagcacgag gacgtgcagg tctccaacgc
    11761 cttcgccaag ctcgattcca tcatgcactc gccctgcgag atgacgatcc ggccggacgg
    11821 caagtggcgg ctggacttcg agggggcgag ccgctggggc tggcacacct tcaacgccca
    11881 ccaggtggca ttcacctcgg
//
LOCUS       DAOQPA010000107        11727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2824_length_11727_cov_19.252228, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000107 DAOQPA010000000
VERSION     DAOQPA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_11727_cov_19.252228"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PKX01_13480"
     CDS             <1..1350
                     /locus_tag="PKX01_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77189.1"
                     /translation="AVWWHHQADLEQRERERDPALAGGPRAGWAVASAAAVLAHGGTP
                     AVLSLLDEWGAEGRAPRLLVVDEAGHDLLGRPVSAEALASARRRADHDHPAAPVRQVL
                     SADGRTYLLFVPADAGNRGWEGVPPGPPPGLDLPGRPPGPPFERRRPPDRPGLGVLIA
                     SGLLASVLVSALLAWYLAKPVRHLRWAFGAVADGRLDTRVSPLMGARRDEIADLGVEF
                     DRMAGQLQALVGAQRRLLHDVSHELRSPLARMQAAIGLARQDASKLPATIDRIERESV
                     RLDELVGQLLTLARLDAGSGDAPEEAVDLIDLVAAIADDARFEAETLGKTVHFVGDGQ
                     ALTRVRAELIQRAFENVIRNAVKFTAVGTAVDVTAGVIDGRFRLRVADRGPGVSESDL
                     ATIFEPFHRGRAAVTAGGFGLGLAIARRAVTSHGGQIEASNRDGGGLEMSIVLPLAG"
     gene            1397..1495
                     /gene="ffs"
                     /locus_tag="PKX01_13485"
     ncRNA           1397..1495
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKX01_13485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1750..2133
                     /locus_tag="PKX01_13490"
     CDS             1750..2133
                     /locus_tag="PKX01_13490"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HMZ77190.1"
                     /translation="MAIYHGSCHCGAVRFAIDTEIIRVTECNCSICRKKGILHHRVAP
                     ERFSLLSGEEVLGTYRFGSGVARHHFCTVCGIHVFTRPRAAPELYTVNVRVLDDYDVT
                     ARQPEVVPFNGREWEASVAALNAPR"
     gene            2238..2486
                     /locus_tag="PKX01_13495"
     CDS             2238..2486
                     /locus_tag="PKX01_13495"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HMZ77191.1"
                     /translation="MLELRPGCECCDRDLPPDSAEARICSFECTFCATCAEEVLGGRC
                     PNCGGELVARPRRPAEKLATHPPSDRRVRRPAGCGTQD"
     gene            2529..2912
                     /locus_tag="PKX01_13500"
     CDS             2529..2912
                     /locus_tag="PKX01_13500"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HMZ77192.1"
                     /translation="MTSDPSDSERRKRRDSEIRCQDEAIGLHLAEALRSGELQTAASF
                     GKPMPEPEGWSETPGEFRMAFKILKNAGAPPPEIDLFHQRARLRAELEAASSDARRQA
                     IRQRLSELEQALALRLEGMRVSGKL"
     gene            3001..4929
                     /gene="dnaX"
                     /locus_tag="PKX01_13505"
     CDS             3001..4929
                     /gene="dnaX"
                     /locus_tag="PKX01_13505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMZ77193.1"
                     /translation="MAYQVLARKWRPRSFETLVGQEHVVRALTHALATGRLHHAYLFT
                     GTRGVGKTTISRILAKALNCETGVTAAPCNQCAACVAIDADRFPDYVEMDAASNRGVD
                     DMAALLDQAVYAPVQGRYKVYMIDEVHMLTGHAFNAMLKTLEEPPEHVKFIIATTDPQ
                     KIPVTVLSRCLQFNLKQMPPGHIVGHLTRLLEAEAVPFEPGALRHLAKAAGGSMRDAL
                     SLLDQAIAHGAGKVEEQAVQDMLGTVGDDHLYAILDALVAQDVQGLMAAAEGMEARSL
                     SFDSALQSLASLLHRIALVQLAPTAVTDEFEAARLAPYAEAFDAEFLQLAYQIAIHGR
                     DELPLAPDEATGFSMSLLRLFAFRPEQPPALGSAAASGGGGTGHTRPLPAATSRGAPA
                     ERPAPSPALSTATPAALRTAPAPTPLPVVAQRSAVPVPQHATQVPAVAPAPEMGSSVP
                     PWEDLPPEAHGFAAAPILEVPPALPVAEAPARPAEPAALRSAVAPDRPVEASPAELPS
                     GDLPPLPAGLDWHALLADLGLGGMVRELAQHCELTGTANGTLRLRLGHTHRHLLAINR
                     SGPEKLQEALAAHFGRPLRVHVEVGEIATETPAQRNEAVRAERHAAAVAALEQDPFVR
                     ELIERFDATMEESSVKPL"
     gene            4951..5274
                     /locus_tag="PKX01_13510"
     CDS             4951..5274
                     /locus_tag="PKX01_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMZ77194.1"
                     /translation="MMKGGIAGLMKQAQQMQENMKKMQDSLGGIEVEGQSGAGMVKVT
                     MTCKYDVRRVSIDPSVMDDKEMLEDLIAAALNDAVRRVESTTQEKMSGFTAGLNLPPG
                     FKLPF"
     gene            5291..5887
                     /gene="recR"
                     /locus_tag="PKX01_13515"
     CDS             5291..5887
                     /gene="recR"
                     /locus_tag="PKX01_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMZ77195.1"
                     /translation="MSSPSSLDTLIEALRCLPGVGPKSATRMAYHLLQRDQRGAARLA
                     TALGHALEVLRHCTRCNTLCEDEVCARCANPRRDGTQLCVVEMPADLAMLEQTHSYQG
                     LYYVLMGRLSPLDGIGPRELHLEKLLARATDGVVEEVILATNFTNEGEATAHMISELL
                     RSRGLRVSRIARGIPVGGELEHTDTGTIAQALVERRAL"
     gene            5931..7121
                     /locus_tag="PKX01_13520"
     CDS             5931..7121
                     /locus_tag="PKX01_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HMZ77196.1"
                     /translation="MDKPLQGVKVLELGTLIAGPFCTRILAEFGADVIKVESPDGGDP
                     LRQWRKLHEGTSLWWAVQARNKRSLTANLKHPDGLAVVRRLAAEADIVVENFRPGVLD
                     RLGLGWEALSAANPGLVMVRLSGFGQTGPYKDRPGFGAIGESMGGLRYITGFPDRPPV
                     RTGISIGDSIAALWAAIGALMALRHREVNGGAGQVVDVALYEAVFAMMESMVPEFDLF
                     GFVRERTGNIMPGITPSNTHTTRDGRHVIIGGNGDAIFLRLMQAIGRPDLAGDPTLAS
                     NAGRDARAAELYAAIDAWCTAHDGDAVLAALEAAQVPASSVFSVADMFRDAQFLARGM
                     IESANLADGTPLRVPGIVPRLTGTPGTTAWLGPTLGEHTDAILADLGYTPAAVTALRE
                     NGAI"
     gene            7194..8213
                     /gene="galE"
                     /locus_tag="PKX01_13525"
     CDS             7194..8213
                     /gene="galE"
                     /locus_tag="PKX01_13525"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMZ77197.1"
                     /translation="MPTILVTGGAGYIGSHTCVELLAAGYDVVVVDNFSNSKPEALKR
                     VEAIAGRPLAAFYRSDIRDKAALREIFRTHAIDAVIHFAALKAVGESVAKPLMYYDNN
                     IAGTVALAEAMSDAGVKSLVFSSSATVYGDPASVPIREDFPTGPTNPYGRTKWMMEYV
                     LSDLAAADPAWRVALLRYFNPVGAHASGRIGEDPNGLPNNLMPFVSQVAVGRLPRLRV
                     FGGDYATVDGTGVRDYIHVVDLAVGHVAAVRRLTERPGVLTVNLGTGRGYSVLEVVQA
                     FERACGRPVPYDIVDRRPGDVAQCYAETTLAAQELGWKAERGIDEMCADAWRWQSSNP
                     QGYPD"
     gene            8210..8599
                     /locus_tag="PKX01_13530"
     CDS             8210..8599
                     /locus_tag="PKX01_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HMZ77198.1"
                     /translation="MSTEDMPVSDALNVLGGPLLACSYDPLTGWMRTGCCEADPADLG
                     RHHVCVKVTAEFLAFSTRRGNDLTTPKPDYRFAGLKPGDRWCLCAFRWREAWEAGVAP
                     PVVLEATHAATLEVIPLEALKRHAYPR"
     gene            complement(8612..10024)
                     /gene="dacB"
                     /locus_tag="PKX01_13535"
     CDS             complement(8612..10024)
                     /gene="dacB"
                     /locus_tag="PKX01_13535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HMZ77199.1"
                     /translation="MPAPFARFLFLCLLSGFVRAETVLPAPVQEALNRASIPRDAVAV
                     WIQDLEAPRPIVTHRVDAAMNPASVMKLVTSQAALELLGPTYVWRTRITSTAPLRRGV
                     LDGDLAITASGDPRLSRERLWLLLRDLRMAGVSRIAGDVITDRSVFSLPPYDPGAFDQ
                     RPLRPYNAPADALSVDYSALRLRISPTTDGAIVRSEPLPAGLSLVSRIRRNAGPCADP
                     SAGLSAGVEIADAGTRLVLDGFVPQDCTAPFDWNVAPLTPARLFEGLFRSLWAEMGGT
                     LDGRFRDGPTPDGGAVLAESVSPALPEVLRDMNKWSNNVIARQLFATLGAVTEPGADS
                     LAAGARACRTALQAAGIDTRTLVMENGSGLSRSERASAALLGQLLIRAWHSPTMPELI
                     ASLPIAGRDGTARRRLAGSTTAGFAHVKTGTLDDVRSLAGYVLARNGRRYGVVLLINH
                     PNASAARGAQDALLEWVANL"
     gene            10274..10867
                     /gene="petA"
                     /locus_tag="PKX01_13540"
     CDS             10274..10867
                     /gene="petA"
                     /locus_tag="PKX01_13540"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HMZ77200.1"
                     /translation="MSVDQKVDASRRQLLLVTSAAGGVAAAAAAVPFVASLTPSERAK
                     AAGAPVEVDISKLQPGEMMTVEWRGKPVWIINRTKEMLDSLKKTDAEVSDPNSDKPQQ
                     PEYAKNETRSIKPEILVAVGICTHLGCSPSSKFKTGAESGVSADWPGGFLCPCHGSTF
                     DLAGRVYKSKPAPDNLEIPPHKYLADTKILIGEDGKA"
     gene            10871..>11727
                     /locus_tag="PKX01_13545"
     CDS             10871..>11727
                     /locus_tag="PKX01_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HMZ77201.1"
                     /translation="MTTKAQALLDWVDARFPLTSSIKGHLTEYYAPKNFNFWYFFGSL
                     ALMVLVIQIVTGIFLVMHYKPDASLNASGVPVAFASVEYIMRDVPGGWIIRYMHSTGA
                     SAFFIVVYLHMFRGLLYGSYRKPRELIWIFGTLIFLALMAEAFMGYLLPWGQMSFWGA
                     QVIVNLFSAIPVIGPDLSVVIRGDFVVSDATLNRFFSFHVIAVPLILIGLVAAHIVAL
                     HEVGSNNPDGVEIKKLKGADGHPLDGIPFHPYYTVKDIVGVVGFLIVFSIIVFFAPEG
                     GGYFLEFNN"
BASE COUNT         1771 a         3946 c         4081 g         1929 t
ORIGIN      
        1 gccgtgtggt ggcatcacca ggccgacctg gagcagcgtg agcgtgagcg ggacccggcc
       61 ctcgctggtg gaccgcgggc cggctgggcg gtggcttccg ccgcggccgt cctcgcccac
      121 ggcggcacgc cggcggtatt gagcctgctc gacgagtggg gggccgaagg gcgcgcgccg
      181 cgcctgttgg tggtggatga ggcgggccac gacctgctcg gtcggcctgt gagcgccgag
      241 gcgctggcga gtgcccgccg cagggccgat cacgatcatc ctgcggcccc ggtgcggcag
      301 gtgctgagcg ccgacggccg gacttacctg ctcttcgtgc cggcggacgc gggcaatcgt
      361 ggctgggagg gggtgccgcc cggcccaccg ccgggccttg atctgccggg tcgcccgccg
      421 ggcccgcctt tcgagcggcg gcgtccgccg gatcgaccgg ggctgggcgt gctgatcgcc
      481 agcggattgc tcgccagcgt gctggtgtcg gcgttgctgg cgtggtatct cgccaagccg
      541 gtccgccatc tgcgctgggc cttcggcgcc gtcgcggacg gccggctcga cacgcgggtg
      601 agcccgctga tgggcgcgcg gcgggacgag atcgccgatc tgggggtgga gttcgatcgc
      661 atggcgggcc agctgcaggc tctcgtcggc gcccagcgtc gcctgctcca cgatgtctcc
      721 cacgagctgc gctcacccct ggcgcgcatg caggcggcca tcgggctggc ccggcaggat
      781 gcgtccaagc tcccggcgac gattgaccgc atcgaacggg agtccgtgcg cctcgacgag
      841 ctcgtggggc agttgctcac cctggcgcgc ctggatgccg gttcggggga tgcgccggag
      901 gaggccgtgg atctgatcga tctggtggct gccatcgcag acgacgcccg tttcgaagcc
      961 gagacgctcg gcaagacggt gcatttcgtg ggggacggac aggcgctgac gcgtgtgcgc
     1021 gccgagttga tccagcgcgc cttcgagaac gtgatccgta atgcggtcaa gttcacggcg
     1081 gtggggacgg ccgtcgatgt cacggcgggc gtcatcgacg ggcgtttccg tctgcgcgtg
     1141 gctgatcggg ggccgggcgt gtcggaaagt gatctggcga ccatcttcga gcccttccat
     1201 cgcggccgcg cggcggtgac ggcggggggc ttcggcctgg ggctggcgat cgcgcgccgt
     1261 gcggtgacct cccacggggg gcagatcgag gcgagcaacc gggacggcgg tggcctggag
     1321 atgagcatcg tcctgccctt ggcaggctga acggggggct gtccgcagtg ggcacgaacc
     1381 tgttagaatt cggcgtggcg ggtctccccg cattgcagcg cggtgaacct ggtcagggcc
     1441 ggaaggcagc agccacagcc gttccctgca agtgccgggg gtcaggctcg ccaccccgaa
     1501 tcgcggaaaa gggcgctctc aggagcgccc tttttcatgc cggatcctgg tcggtcggcc
     1561 cgcgcagggg gtatcgtacg gacgtttgcg ggcggcaacc cggtctggcg cggaggtggt
     1621 ccgcgcgctg cgcaacgccg ggctgttctc cgtggttcgg cggcgcctgc tgcgcggcgt
     1681 ggaggcgagc gacaatgcgc gcctcagtgc tgacgggcgc cgtggcgttt cagccgggaa
     1741 ggagttcgca tggcgatcta tcatggaagc tgtcattgcg gcgcggtacg ttttgcaatc
     1801 gacacggaga tcatccgcgt gaccgaatgc aattgctcca tctgccgcaa gaagggcatc
     1861 ctgcaccacc gggtggcgcc ggagcgcttc agcctgttga gcggcgagga ggtgcttggc
     1921 acctaccggt tcggttccgg cgtggcgcgc catcatttct gtaccgtgtg cggcatccac
     1981 gtattcacac ggccgcgggc ggcgccggaa ctctatacgg tgaatgtgcg ggtactggac
     2041 gactacgatg tgaccgcgcg gcagcccgag gtggtgccct ttaatggtcg ggaatgggaa
     2101 gccagcgtgg cggcgttgaa cgcgccccgc taggacgctc cggccctcgc gtcccgtttt
     2161 ctggtacacc gcccccgtcg tgacggggat gttcatcaac gggacgacac cggtcgtcct
     2221 catccgggag cgtcgccatg cttgaacttc gaccgggctg tgaatgctgt gaccgggatc
     2281 ttccgcccga ctccgccgag gcccgcatct gctcgttcga atgcaccttt tgtgcgacct
     2341 gcgcagaaga ggtgctcggc ggccgctgcc ccaattgcgg cggcgaattg gtggcgcggc
     2401 cgcggcgtcc ggccgagaag ctcgccacgc acccgccatc cgatcgccgg gtgcgtcgcc
     2461 ccgccggctg cgggacgcag gactgatccg gtcgggttgg ggcggcgctg cccggccgtt
     2521 ggggtgccat gacatccgat ccttccgatt ccgaacggcg caagcgccgc gatagcgaga
     2581 tccgttgcca ggacgaggcc atcggcctgc acctggccga ggccttgcgg tcgggtgagt
     2641 tgcagaccgc tgccagcttc ggcaagccga tgccggagcc ggagggctgg tccgagaccc
     2701 cgggcgagtt ccgcatggcc ttcaagatcc tcaagaatgc cggtgcgccg ccgccggaga
     2761 tcgacctctt ccaccagcgc gcgcgcctgc gggccgagct ggaggcggcg tcctcggatg
     2821 cgcggcgaca ggcgatccgg caacgtctca gcgagctgga gcaggccctt gcgctacgtc
     2881 tggagggcat gcgggtgagc ggcaagctgt agcggcgtgc ccgcggcggg cacctgagtg
     2941 cgtccgaggg gcggatgcgc ggtttcccgg agcaggctgc cggcctgata gaatccggcc
     3001 atggcttatc aagtacttgc ccgcaagtgg cgcccgcgca gtttcgagac gctcgtcggg
     3061 caggagcacg tggtgcgtgc cctgacccac gccctggcca ccggccggct tcaccacgcc
     3121 tacctgttca ccggcacccg aggggtgggc aagaccacca tctcgcgtat cctcgccaag
     3181 gccctcaact gtgagacggg cgtcaccgcc gcgccgtgca accagtgcgc cgcctgcgtc
     3241 gccatcgacg ccgaccgctt tcccgactac gtggagatgg acgccgcctc caaccgcggc
     3301 gtggacgaca tggcggcgct gctcgatcag gccgtgtatg caccggtgca gggccgctac
     3361 aaggtctaca tgattgacga agtgcacatg ctcaccgggc atgccttcaa cgccatgctc
     3421 aagaccctgg aagagccgcc ggagcacgtc aagttcatca tcgccaccac cgacccgcag
     3481 aagatcccgg tcacggtgct gtcccgctgc ctgcagttca acctcaagca gatgccgccg
     3541 gggcacatcg tcggccacct gacgcggctg ctggaagccg aggccgtgcc cttcgagccg
     3601 ggtgcgctgc gtcacctggc caaggcggcg gggggctcca tgcgcgacgc cctgtccctg
     3661 ctcgaccagg ccatcgccca cggcgccggc aaggtcgaag agcaggctgt gcaggacatg
     3721 ctgggcacgg tgggcgacga tcacctctac gccattctgg atgccctggt ggcccaggac
     3781 gtgcagggcc tgatggctgc agccgaaggc atggaggcac gcagcctgtc cttcgattcc
     3841 gccctgcagt cccttgccag cctgctccat cgcattgcgc tggtgcaact ggcacccacc
     3901 gcggtgaccg acgaattcga ggccgcccgg ctggcgccct acgccgaggc ctttgacgcc
     3961 gagttcctgc agctggccta ccagatcgcc atccacggcc gcgacgagct gcccctcgcg
     4021 cccgacgagg caaccggctt ctccatgagc ctgctgcgtc tgtttgcctt ccgccccgag
     4081 cagccgccgg ccctgggcag cgcggccgcc tccggcggtg gcgggacggg acacacgcgt
     4141 ccgctgccgg ctgcgacgag tcgaggcgcg cctgcggagc gtcctgcgcc ctctccggcg
     4201 ctgtcgacgg ccacgccggc cgcgctccgt acggcgcctg ccccgacgcc tttgccggtg
     4261 gtggcacagc gctcggcggt acccgttccg cagcacgcca cgcaggttcc cgcggtcgcg
     4321 cctgcgccgg aaatgggcag cagcgtgccg ccctgggagg atctgccgcc ggaggcccat
     4381 ggttttgcag cggcacccat cctggaggtc ccgcccgccc tgcctgtggc cgaagcgccc
     4441 gcgcgtccgg cggaaccggc tgcgctgcgc tcggccgttg cgcccgaccg gcccgtcgag
     4501 gcgtcgcctg cagaactgcc gtccggtgat ctccccccgc tgccggccgg cctcgactgg
     4561 catgccctgc tggctgatct cggcctcggc ggcatggtgc gcgagctggc ccagcactgc
     4621 gagctgaccg gcaccgccaa cggcacgctg cgtctgcgcc tcggtcacac ccaccgccac
     4681 ctgctcgcca tcaaccgcag cggtcccgaa aagctgcagg aggccctggc cgcccatttc
     4741 ggccggcccc tgcgggtgca cgtggaagtc ggcgagatcg ccaccgaaac ccctgcccag
     4801 cgcaacgagg ccgtgcgggc cgagcgccac gcggcggcgg tggcggcgct ggagcaggat
     4861 ccctttgttc gcgaactgat cgaacgtttc gatgcgacga tggaagaatc gtcggtcaag
     4921 cccctttaac ttcaacggag tcaatgcgcc atgatgaaag gcggaatcgc cggcctgatg
     4981 aaacaggccc agcagatgca ggaaaacatg aagaagatgc aggacagcct cggcggcatc
     5041 gaggtcgaag gccagtccgg cgcaggcatg gtgaaggtga ctatgacctg caagtacgac
     5101 gtgcgccggg tcagcatcga cccgtcggtg atggacgaca aggagatgct cgaggacctg
     5161 atcgccgcgg ctctcaacga cgccgtgcgc cgtgtcgagt ccaccaccca ggaaaagatg
     5221 tccggcttca ccgccggcct caacctgccg ccgggcttca agctgccgtt ctgaggcgtt
     5281 ggccgcgtcc atgagcagcc cgtccagcct cgataccttg atcgaagccc tgcgctgcct
     5341 gcccggcgtg gggcccaagt cggcaacccg catggcttac cacctgctcc agcgcgatca
     5401 gcgcggtgcg gcgcggctgg ccacggccct gggccatgcg ctggaggtgc tgcgccactg
     5461 cacccgctgc aatacgctgt gcgaggacga ggtctgtgcc cgctgcgcca acccgcggcg
     5521 cgacgggacg caactgtgcg tggtggagat gcccgctgat ctggcgatgc tggagcagac
     5581 ccacagctac cagggcctgt attacgtgct gatggggcgc ctgtcgcccc tggacggcat
     5641 cggaccgcgg gagctgcatc tggaaaagct ccttgcccgg gcgacggacg gcgtggtgga
     5701 ggaggtcatc ctcgccacca atttcaccaa cgagggcgaa gctacggccc acatgatctc
     5761 cgagctgctg cgcagccgcg ggctcagggt gagccgcatc gcccgcggca ttccggtggg
     5821 cggcgaactg gaacacaccg acacgggcac catcgcccag gccctggtgg agcggcgcgc
     5881 cctgtagggc gagcgctgtg ccgcggccgg gcgcccgatc aggacatccc atggacaaac
     5941 cccttcaggg cgtgaaggtg ctggagctgg gcaccctcat cgccggcccc ttctgtaccc
     6001 gcatcctggc cgaattcggc gccgacgtga tcaaggtgga atcgcccgac ggcggcgatc
     6061 ccctgcgcca gtggcgcaag ctgcacgaag gcacctccct gtggtgggcc gtgcaggcac
     6121 gcaacaagcg ctccctcacc gccaacctca agcatccgga tggcctggcc gtcgtgcgcc
     6181 ggctggcggc ggaggcggac atcgtcgtgg agaacttccg ccccggcgtg ctggaccgcc
     6241 tcgggttggg ctgggaggcg ctgtcggcgg cgaatccggg gctggtgatg gtgcgtctgt
     6301 cgggcttcgg ccagaccggg ccctacaagg atcggccagg tttcggggcg atcggcgagt
     6361 ccatgggcgg gttgcgctac atcaccggct ttccggaccg gccgcccgtg cgcaccggga
     6421 tttccatcgg cgactccatt gccgccctgt gggccgccat cggcgccttg atggccctgc
     6481 gtcaccggga ggtgaatggc ggcgccgggc aggtggtgga cgtggcgctc tacgaggcgg
     6541 tcttcgcgat gatggagagc atggtgcccg agttcgacct gttcggtttc gtgcgcgagc
     6601 ggaccggcaa cattatgccg ggcatcaccc cgtccaacac ccataccacc cgcgacggcc
     6661 gccacgtgat catcggcggc aacggggatg ccatcttcct gcgtctgatg caggccattg
     6721 ggcggcctga cctggcgggc gacccgaccc tcgccagcaa cgccggacgc gacgcgcggg
     6781 cggcggagct ctacgccgcc atcgacgcct ggtgcacggc ccatgacggt gacgcggtgc
     6841 tcgccgcgct ggaagccgcc caggtgccgg ccagctccgt cttctcggtg gccgacatgt
     6901 tccgcgatgc ccagtttctg gcgcggggca tgatcgaatc cgcgaacctg gccgatggca
     6961 cgcccctcag ggttccgggc atcgtgcccc ggctgaccgg gactccgggc accaccgcat
     7021 ggctggggcc gacgctgggt gagcacaccg atgccatcct ggccgatctg ggctacacgc
     7081 cggccgccgt gaccgccttg cgggagaatg gcgcgatctg atgaatatgt ggcgatgtcc
     7141 tgtcaggacg acgggctatc attgcgcttt ctgttacttg aatggaattt ttcatgccga
     7201 caatcctggt gaccggcggc gccggctaca tcggttccca cacctgcgtg gagctgctcg
     7261 cggcgggcta cgacgtggtg gtggtggaca acttcagcaa cagcaagccc gaggccctga
     7321 agcgggttga ggcgatcgct ggccgtccgc tggcggcctt ctaccgcagc gacatccgtg
     7381 acaaggccgc cctgcgggag atcttccgca cccatgccat cgacgcggtg attcattttg
     7441 cggccctcaa ggcggtgggc gaatcggtcg ccaagccgct gatgtactac gacaacaaca
     7501 tcgccggcac ggtggccctg gctgaggcga tgagcgacgc gggggtcaag tccctggtgt
     7561 tcagctcttc cgccacggtg tacggcgatc cggccagcgt gccgatccgc gaagatttcc
     7621 cgaccgggcc gaccaatccc tacggacgca ccaagtggat gatggaatac gtgctgtccg
     7681 atctcgcggc ggccgatccc gcctggcggg tggcgctgct gcgctacttc aacccggtgg
     7741 gcgcccacgc cagcgggcgc atcggcgaag atcccaatgg cctgcccaac aacctgatgc
     7801 cctttgtgag ccaggtggcg gtgggtcgtc tgccgcgcct gcgggtgttc ggcggcgatt
     7861 acgccacggt ggacggtacc ggcgtgcgcg actacatcca cgtggtggat ctggcggtcg
     7921 gtcacgtggc ggcggtgcgg cgcctgaccg aacggcccgg cgtgctgacg gtcaaccttg
     7981 gcaccgggcg cggctacagc gtgctggagg tcgtgcaggc cttcgagcgg gcctgtggcc
     8041 gtccggtacc ctacgacatc gtcgaccgcc gtcccggtga cgtggcccag tgctatgccg
     8101 agaccaccct cgccgcgcag gaactgggct ggaaggccga gcggggcatc gacgagatgt
     8161 gcgccgacgc gtggcgctgg cagagttcca atccccaggg gtatcccgat tgagcaccga
     8221 agacatgcct gtgagcgacg ccctcaacgt gctgggcggg ccgctcctcg cctgcagcta
     8281 cgatccgctg accggctgga tgcgcaccgg ctgctgcgag gcggaccccg ccgatctggg
     8341 gcgtcaccac gtgtgtgtca aggtgacggc cgagttcctg gccttcagca cgcggcgcgg
     8401 caatgatctg accacgccca agcccgatta tcgtttcgct gggctcaagc ccggcgaccg
     8461 ttggtgcctg tgtgctttcc gctggcgtga ggcctgggag gcgggcgtcg cgccgcccgt
     8521 cgtgctggag gcaacccatg cagcgacgct ggaggtcatc cccctcgagg ccctcaagcg
     8581 ccacgcctac ccgcgttgag accccgcccc gctaaaggtt cgcgacccac tccagcaggg
     8641 cgtcctgggc gccccgtgcc gcgctggcgt tggggtggtt gatgagcagc acgacaccgt
     8701 agcgcctgcc gttcctggcc agcacgtagc cggccaggct gcgcacgtcg tccagggtgc
     8761 cggtcttcac atgggcgaag ccggccgtcg tggagcctgc gaggcgacgg cgggccgtgc
     8821 cgtcgcgccc ggcaatgggc agggaggcga tcaactccgg catggtcggg ctgtgccagg
     8881 cgcggatcag caactggccg agaagggcgg cgctggcccg ttccgatcgc gacaggcccg
     8941 agccgttttc catcaccaac gtgcgcgtgt cgatgcccgc ggcctgcagc gcagtgcggc
     9001 acgcacgggc gccggctgcc agactgtcgg cgccgggttc cgtcacggca cccagagtgg
     9061 cgaaaagctg tcgggcgatg acgttgttgg accacttgtt catgtcccgc agcacctcgg
     9121 gcagcgcggg cgagacgctc tcggcgagga ctgcgccgcc gtcgggcgtc ggaccgtccc
     9181 ggaagcgccc gtccagcgtg ccgcccattt ccgcccagag gctgcggaac aggccttcaa
     9241 acaggcgtgc gggcgtcagg ggcgcgacgt tccagtcgaa tggcgctgtg cagtcttggg
     9301 ggacgaaccc gtcgaggacc agccgggtgc ccgcatcggc gatctccacg ccggcgctca
     9361 gcccggccga cggatcggcg caggggcccg cgttccggcg gatgcgactg acaaggctca
     9421 atccggccgg caggggctcg ctgcggacga tggcgccgtc ggtcgtgggg ctgatgcgca
     9481 ggcgcagcgc gctgtaatcg acgctcaggg catcggccgg cgcgttgtag gggcgcagcg
     9541 gccgctggtc gaaggccccc ggatcgtagg ggggcaggct gaagacgctg cggtcggtga
     9601 tcacgtcgcc ggcgatgcgc gagacgccgg ccatgcgcag gtcccgcagg agcagccaca
     9661 gacgttcccg tgacaggcgc gggtcgccgc tcgcggtaat ggccagatcg ccgtccagca
     9721 cgccccgccg aagcggcgcc gtggacgtga tgcgggtgcg ccacacgtag gtcggcccga
     9781 gcagttccag tgcagcctgg ctggtgacca gcttcattac cgacgcaggg ttcatggctg
     9841 cgtcgacacg atgggtgacg atggggcgtg gcgcttccag atcctggatc cacacggcaa
     9901 ccgcatcgcg cggaatggac gcacgattca gggcttcctg tacgggcgca gggagcacgg
     9961 tttcggcccg gacgaatccg ctcaggaggc acaggaacag gaaacgggcg aatggtgcag
    10021 gcatggtcgg agtgcggacg gcaatggaca cgattatgcc tgccgagggc gcgcctgcag
    10081 cattgtgcgc cgcaacacga cgcctcggac gggccgcgat ggcgcgcctt tggcagcacc
    10141 gcggacgccc aaatttgcct gatccggcgc ggctttggcc cataaggctt ttttcaggta
    10201 agggttttcg ctatactccg cgagttttcc gtattgcgat cttccaagtc tcttttatcg
    10261 aggttagtgg gccatgagcg tggatcaaaa agtggatgcg agccggcgcc agctcttgct
    10321 ggtgacatcg gcggcgggcg gggtggcggc agccgctgcg gccgtgccgt tcgtcgcgag
    10381 cctgacgccg tccgagcgag ccaaggcggc cggtgcgccg gtcgaggtgg atatcagcaa
    10441 gctgcaaccc ggtgagatga tgaccgtgga gtggcggggc aagccggtgt ggatcattaa
    10501 ccgcaccaag gagatgctgg actccctgaa aaagacggat gccgaggtca gcgacccgaa
    10561 ttccgacaag ccccagcagc ccgagtacgc caagaacgaa acccgttcca tcaagcccga
    10621 gatcctcgtg gcggtgggca tctgtaccca cctgggctgc tccccgtcgt cgaagttcaa
    10681 gacgggtgcc gagagtggcg tcagtgcaga ctggcccggc ggtttcctgt gcccgtgtca
    10741 cggctccacc ttcgacctgg caggccgcgt ctacaagagc aagccggcgc cggacaatct
    10801 ggagatcccg ccgcacaagt acctggccga caccaagatc ctgatcggcg aagacggaaa
    10861 ggcctaaacg atgaccacca aagctcaagc attgctcgat tgggtcgacg cgcggttccc
    10921 gctgacgtcg tccatcaagg gccacctgac cgagtactac gcgccgaaga acttcaactt
    10981 ctggtacttc ttcggatctc tggccctgat ggtgctggtg atccagatcg tcaccggcat
    11041 cttcctggtg atgcactaca agccggacgc ctccctgaac gcttccggcg tgccggtggc
    11101 cttcgcctcc gtcgagtaca tcatgcgcga tgtgcccggc ggctggatca tccgctacat
    11161 gcactccacc ggcgcctccg cgttcttcat cgtggtctac ctccacatgt tccgcggcct
    11221 gctctacggt tcctaccgca agccccgcga actgatctgg atcttcggca ccctgatctt
    11281 cctggcgctg atggctgaag ccttcatggg ctatctgctg ccatgggggc agatgtcctt
    11341 ctggggcgcc caggtgatcg tgaacctgtt ctccgcgatt cccgtgatcg gcccggatct
    11401 gtccgtggtc atccgcggcg acttcgtggt gtccgatgcc accctgaacc gcttcttctc
    11461 cttccacgtc atcgccgtgc cgctgatcct catcggcctg gtggcggcgc acatcgttgc
    11521 cctgcacgaa gtgggctcca acaacccgga cggtgtcgag atcaagaagc tcaagggcgc
    11581 ggacggccat cctctggacg gcatcccctt ccacccctac tacaccgtca aggacatcgt
    11641 gggcgtggtc ggtttcctga tcgtcttttc catcatcgtg ttcttcgcgc ctgaaggcgg
    11701 cggctacttc ctggagttca acaactt
//
LOCUS       DAOQPA010000108        11374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2941_length_11374_cov_16.053980, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000108 DAOQPA010000000
VERSION     DAOQPA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2941_length_11374_cov_16.053980"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKX01_13550"
     CDS             complement(<1..444)
                     /locus_tag="PKX01_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77202.1"
                     /translation="MNTTARPLPPLLLGLFRELQANGVPVGVRDYLDGLRALALCTGA
                     DADPALRDAFGRPARDSLRDLALALWARSDEERRLITRWFDVLPEPADALVQALDACL
                     ETLDTPEDTAAAAPGDLPARPATPPPDLTAGAGATPPPSAVRSGGG"
     gene            complement(441..1379)
                     /locus_tag="PKX01_13555"
     CDS             complement(441..1379)
                     /locus_tag="PKX01_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ77203.1"
                     /translation="MSATPYKSFTGTATDFYVAAPATATLVNLAVRLGRPILVEGEAG
                     CGKTRLASAIATELGVGLTVMTVKSTSQAKDLLYRFDALRRLQDAQIPGNTTARHVHP
                     YLSLEPLGHAIQSGTPQVVLIDEVDKADIDFPNDLLDVLDRFEFDLEDLPRDEEDACR
                     AARGFGRHVSAPKDGTRPIVLITSNREKQLPEPFLRRCLYLQLDFPEDQPERLADIVR
                     KNLAPRGESVGDTLLAAAVARFCEIRAQGRRQGVRKLPATSELVDWVRTLHWQGRKED
                     SLDPKALDALDTAVLFKLRADIDAYKAGKDEDGGGW"
     gene            complement(1391..2803)
                     /locus_tag="PKX01_13560"
     CDS             complement(1391..2803)
                     /locus_tag="PKX01_13560"
                     /inference="COORDINATES: protein motif:HMM:NF040412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HMZ77204.1"
                     /translation="MQTGGTRPAQRPPHPPEPMPRAADLPKIERIFLSATATDCLAYR
                     EAVKAAVEGNLRAAKIFLQEKWALNAETAVKACKDRLLDCDAYVGLFGHRYGHIAPGQ
                     TISITEQEFRWAVEHWPHLTPPIFILRAKPGSEADTFLRAQAEQCWHTLPTPEAVEAD
                     RSAQQRFVAAVTDWSAEEGRVLNFYADLIELRDKAQACINHWNLDLLEAALDGRYQAT
                     GEIPAAELGRLGRKPQFTAFRDSLDTFRERRDQRAVAFLVHGPENHGQPQFMEALAQW
                     TTGKDGMEVFAGQASEPDSAESLICWLCSRLNEPLLGEPTVDALARILAKRLERRTVV
                     VLLHTVGREAQRLALFHQRFWQPLADALAALKPGGRSRFICVVADHAPLPAAPGAFIR
                     PADPDDADTDYRQLLALPALAPLSARQVRSWLADLEDAGIVLDDDRRDAIAEHATTPD
                     GLPPNVYYRLNLEGFWKHAR"
     gene            2881..3768
                     /locus_tag="PKX01_13565"
     CDS             2881..3768
                     /locus_tag="PKX01_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ77205.1"
                     /translation="MRIGIDLGGTKIEIIALDAAGRTLARRRVATPQGDYAATVQAVA
                     GLVAAVESELGQRGTVGVGTPGALSRVTGRIKNANSTCLNGQPLQDDLQAALGRPLRI
                     ANDANCFALSEAVDGAAAGAETVFGVILGTGVGGGIVVRGQALTGANAIAGEWGHNPL
                     PGGAAPGPACYCGRSGCVETWLSGPGLAADHLRVTGRTCTPEALVAGAAAGDADSIAT
                     LDRYAARLARALATVVNLLDPDVIVLGGGLSNLAMLYEAVPRLWVPHVFSDAIATRLC
                     RAVHGDSSGVRGAAWLWPD"
     gene            3874..4542
                     /locus_tag="PKX01_13570"
     CDS             3874..4542
                     /locus_tag="PKX01_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HMZ77206.1"
                     /translation="MSTPLAVFAGGLRALPPEGQQTGMFKAPVVGPVAVGVDGLAGDR
                     QADRRVHGGPEKAVHYFPAEHYAHLTTRVPALADALRPGVLGENLSARGLTEDTVCIG
                     DVFAAGSCRLQVSQPRNPCWKINHRLDHDRMSRIIVDDGCTGWYFRVLAPGVLTAGEV
                     LRLVERPAPGMTLARLAAVKGAHRPSPDELLAVAAAPGLAPDVARRLQARGAWLTNSG
                     VPPC"
     gene            4615..5064
                     /locus_tag="PKX01_13575"
     CDS             4615..5064
                     /locus_tag="PKX01_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77207.1"
                     /translation="MNPSLEPPRHRAFCTAEFAGRFFLAQLDVSQRMGNLYALLGMGL
                     TDCALRSHVLRATHDAAYTVDLNELDSRKALLLEASLEGFATILRNFAEEVAAAHVRC
                     LEQALGRVPSGAPSALPPALLPFLQPDFAGFGRAFNEALRERAEGRG"
     gene            5189..6445
                     /locus_tag="PKX01_13580"
     CDS             5189..6445
                     /locus_tag="PKX01_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ77208.1"
                     /translation="MTPVPSPPAPPVAPPSAPFLVSRIFLPFALGYYLSYLLRTVNAV
                     ISPDLTRELGLNAADLGLLTSTYFFAFGLAQIPVGIALDRFGPRRVESALLLITALGA
                     TLFALGEDLPVLAGARGLIGFGVSACLMGGLKGFTLWFPRERLASLTGYIMSGGALGA
                     VTASAPLEAALPFIGWRGAFWAVAVLAAGIAALIFFAMPEKSSGHGGGDLQAALRGVK
                     EVLSARRFWSFSGQAAFFTGGFMALQGLWAVPWLMQVNGYSRAVAADHLFWLNLGMLA
                     GQLSIGAWATRLAARGITPLRLMQGGLFLTMLVEALIIARLGPTQPLWCLLGIVSATG
                     AQMYGVVAGLFPLHLSGRVTTSINLMAFVGAFAVQWGLGGLLDGLRAGGMSAATSLEV
                     AFLILLAAQAASFVPLSRAGRYTPVH"
     gene            6548..7354
                     /locus_tag="PKX01_13585"
     CDS             6548..7354
                     /locus_tag="PKX01_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ77209.1"
                     /translation="MSSRQLYVHRYPAAHPEGRPTLLFVHGAYSDGRLWVEHFIPWFR
                     EAGYTCLAVDLSGHGRSEGRDDLDSLGLDDYVDDVAQVVATLRAPPVLIAHSMGSLVA
                     QRYAQRAPLAGMALLAPVPPTGTGGSAGRLALMLPEFFEELPRAIAGSAGPRTLEVMA
                     RVYFSPEMPARETAHYLPLLQAESERAVLEMVTLPLRGLSCRPRLPVLVMGGSQDAVF
                     PASMLCFTALPWRAQQDVVSGAGHMLMLDPQWEDAATRIAAWLETLPAAT"
     gene            complement(7385..7768)
                     /locus_tag="PKX01_13590"
     CDS             complement(7385..7768)
                     /locus_tag="PKX01_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ77210.1"
                     /translation="MPTPIAPPQPALIHFADPAPAASEDRPAPERALGPPPRRLTAER
                     YSAEDGALSIGEWTCEPGAWRIAFHAGRHEFFQVLSGRLRLIDDAGHARDFGPGDACV
                     IPAGFKGVFEVLETVTKRYVMVDRA"
     gene            complement(7791..8357)
                     /locus_tag="PKX01_13595"
     CDS             complement(7791..8357)
                     /locus_tag="PKX01_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77211.1"
                     /translation="MSEHDTRRGTSAHTPDLDWSQVRETVLMLELAVGQIEAAMKEGH
                     SSVEVLTDSFTSMAGYMRMIAGALEQLPDTPATQNLKDNLIGHAGEVSGRIQRSIIAF
                     QFYDKLTQRLAHVSHSLEALSGLVVDQRKLYNPFEWVALQEKIRAKYSTREEVDMFNA
                     VMQGMPVKDALEKYKAGLKDKGDDVELF"
     gene            complement(8418..9467)
                     /gene="murB"
                     /locus_tag="PKX01_13600"
     CDS             complement(8418..9467)
                     /gene="murB"
                     /locus_tag="PKX01_13600"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMZ77212.1"
                     /translation="MTASADLLQQDVALDAFNTLRLPARAAWYARVDHVDGLRAVLDD
                     PRVAGLPRLVLGGGSNLVLAGDFAGLVLHVAFHGRSKVQEDADAYYVRAGGGESWHDF
                     VRWTLEQGWPGLENLSLIPGTVGAAPVQNIGAYGLEMAEHFYSLRAYDLATGKTVGFD
                     KRDCGFGYRDSFFKQAGAGRYLIAAVTFRLPKAWKPVTGYADVAAELAARGLVQPTPL
                     QISDAVIAIRRRKLPDPAEIGNAGSFFKNPVVDAATYARLAAAHPGLPHYPQADGREK
                     LAAGWLIDQAGWKGRRLGPVGCYDRQALVLVNHGGATGADVIAVARAVRADVAARFGV
                     DLEPEPVFAGTPPTP"
     gene            complement(9482..10330)
                     /gene="queF"
                     /locus_tag="PKX01_13605"
     CDS             complement(9482..10330)
                     /gene="queF"
                     /locus_tag="PKX01_13605"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987950.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HMZ77213.1"
                     /translation="MSTLPPPAHPDASPLGKPVAYAEHYDPSLLFPILRQGKRDELGL
                     AAGSLPFVGEDVWNAYELSWLNPRGKPEVAVARFVFPADSPCLVESKSLKLYLNAFNQ
                     TPVASADALAGMLAADLSAAAGAPVAVTVEPLSARPQRDTGYPEGVLLDSQDIAIDRY
                     TPAPELLRADTAAPAVSETLYSHLLKSNCLVTGQPDWAMLVVRYRGAPIDREGLLRYI
                     VSFRRHNEFHEQCVERVFTDILARCRPAELCVWARYTRRGGLDINPCRRTPGLPPPPL
                     LAEIRQ"
     gene            10484..>11374
                     /locus_tag="PKX01_13610"
     CDS             10484..>11374
                     /locus_tag="PKX01_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ77214.1"
                     /translation="MNALPIRYVDPVYRPPSEAQSLILPVTDGCSWNHCTFCEMYTDP
                     QKAFRARSEDEVLASIRATGERYGDQMRRVFLADGDALVLPTRRLLRILEAIRQHLPA
                     VHRVSSYCLARNLRRKTVAELRALREAGLALAYLGAESGDDTVLQRVNKGETFDSTRA
                     ALDLLGEAGIGRSVMILNGLGGPLFSRAHAEHSARLANATQPEYLSTLVVSFPQGEAR
                     FRAGFPEWAPLDPPALFREMETFLTALDLHRTVFRSDHASNWLVLKGTLGADKDRLLA
                     QVRAALERPDAAALRPAWARG"
BASE COUNT         1429 a         3860 c         4277 g         1808 t
ORIGIN      
        1 cccgccgcca gacctgaccg cggacggggg aggcgttgcg ccggcgcccg cggtcaggtc
       61 tggcggcggg gtggccgggc gggcggggag atcgccggga gccgccgcgg ccgtgtcctc
      121 aggggtgtcc aaggtctcca ggcaggcgtc aagggcctgg acgagggcgt cggccggttc
      181 gggcagcacg tcgaaccagc gggtgatgag gcggcgttcc tcgtcgctgc gggcccacag
      241 ggcgagggcc aggtcgcgca ggctgtcgcg cgcggggcgg ccgaaggcgt cccgcagggc
      301 cgggtcggcg tccgcgccgg tgcacagggc gagggcgcgc aggccgtcca ggtagtcgcg
      361 gacgcccacc ggcacgccat tggcctgcag ctcgcggaac aggcccagga ggagcggcgg
      421 cagggggcgc gcggtggtgt tcaccagccg cctccgtcct cgtccttgcc ggccttgtag
      481 gcgtcgatgt cggcgcggag cttgaagagg accgcggtgt ccagggcgtc cagggccttg
      541 gggtcgaggc tgtcttcctt gcggccctgc cagtgcaggg tgcgcaccca gtccaccagt
      601 tcgctggtgg cggggagctt gcggacgccc tggcggcggc cctgggcgcg gatctcgcaa
      661 aagcgggcga cggccgcggc gaggagggtg tcgcccacgc tttcgccgcg gggggcgagg
      721 ttcttgcgca cgatgtcggc gaggcgctcg ggctggtctt cggggaagtc cagctgcagg
      781 tagaggcagc ggcgcaggaa gggttcgggg agctgtttct cccggttgct ggtgatgagg
      841 acgatgggcc gcgtgccgtc cttgggtgcg ctgacgtggc ggccgaagcc gcgggcggcg
      901 cggcaggcgt cctcttcgtc gcggggcagg tcctcaaggt cgaattcgaa gcggtcgagg
      961 acgtccagca ggtcgttggg gaagtcgatg tcggccttgt ccacctcgtc gatgagcacc
     1021 acctggggcg tgccggactg gatggcgtgg ccgaggggtt ccaggctgag gtagggatgg
     1081 acgtggcggg ccgtggtgtt gccggggatc tgggcgtcct gcaggcggcg cagggcgtcg
     1141 aagcggtaga gcaggtcctt ggcctggctg gtgctcttga cggtcatcac ggtgaggccg
     1201 acgcccagct cggtggcgat ggcgctggcg aggcgggtct tgccgcagcc ggcctcccct
     1261 tccacgagga tcgggcggcc caggcggacg gccagattga cgagggtggc cgtggcgggg
     1321 gcggccacgt agaagtcggt ggcggtgccg gtgaaggatt tgtacggggt ggcggacacg
     1381 gtggggttcc ttatcgggcg tgtttccaga agccttccag gttcaggcgg tagtagacgt
     1441 tgggcggcag gccgtcgggc gtggtggcgt gctcggcgat ggcgtcgcgg cggtcgtcgt
     1501 cgaggacgat gccggcgtcc tccaggtcgg ccagccagct gcgcacctgg cgggcgctga
     1561 ggggggcgag ggcgggcagg gcgaggagct gacgatagtc cgtgtcggcg tcgtcgggat
     1621 cggcagggcg gatgaacgcg cccggcgcgg caggcagcgg ggcgtgatcg gcgacgacgc
     1681 agatgaagcg gctgcgcccg cccggcttga gggcagcgag ggcgtcggcc aggggctgcc
     1741 agaagcgctg gtggaacagg gcgaggcgct gcgcctcgcg gccgacggtg tggagcagca
     1801 cgaccaccgt gcggcgctcc agacgtttgg cgaggatgcg cgcgagggcg tcgacggtcg
     1861 gttcgcccag caggggttcg ttgaggcggc tgcacagcca gcagatcagg gattcggcgc
     1921 tgtcgggctc gctcgcctgg ccggcgaaga cctccatgcc gtccttgccc gtcgtccact
     1981 gggcgagggc ttccatgaac tggggctggc cgtggttttc cgggccgtgg acgaggaagg
     2041 cgacggcccg ctggtccctg cgctcgcgga aggtgtcgag gctgtcgcgg aaggcggtga
     2101 actggggttt gcgcccgagg cggcccagct ctgcggcggg aatctcgccg gtcgcctggt
     2161 agcggccgtc gagggcggct tcgaggagat ccaggttcca gtggttgatg caggcctggg
     2221 ccttgtcgcg cagctcgatg aggtcggcgt agaagttgag gacgcggccc tcctcggcgc
     2281 tccagtcggt caccgcggcg acgaagcgct gctgggcgct gcggtcggcc tcgacggctt
     2341 ccggtgtggg cagggtgtgc cagcactgct cggcctgcgc gcgcaaaaag gtgtcggcct
     2401 cgctgcccgg cttggcgcgc aggatgaaga tgggcggcgt gaggtggggc cagtgctcca
     2461 ccgcccagcg gaattcctgt tcggtgatgg agatggtctg gccgggcgcg atgtggccgt
     2521 agcggtggcc gaacaggccg acgtaggcgt cgcagtcgag gaggcggtcc ttgcaggcct
     2581 tgaccgcggt ctcggcgttg agggcccact tttcctgcag gaagatcttg gcggcccgca
     2641 ggttgccttc caccgccgcc ttgaccgcct cccggtaggc gaggcagtcg gtggccgtgg
     2701 cgctcaggaa gatgcgctcg atcttgggca ggtcggcggc gcggggcatg ggctcaggag
     2761 gatggggcgg gcgctgggcg gggcgggtgc ctccggtctg catagcatag gccagtcgcc
     2821 cgccccgcgc cacgctgccc agggcttatg atagggcgat cttttgcgga gcgttcctcc
     2881 atgcgtatcg gcatcgacct cggcggcacc aagatcgaaa tcattgccct cgacgcggcg
     2941 gggcggaccc tcgcccggcg ccgcgtggcc acgccccagg gcgactatgc ggccaccgtg
     3001 caggcggtgg cgggcctggt ggcggcggtg gaaagcgagc tggggcagcg cggcacggtg
     3061 ggcgtgggca cgccgggggc gctgtcgcgg gtcaccgggc gcatcaagaa cgccaattcc
     3121 acctgcctca acggccagcc cctgcaggac gacctgcagg ccgccctggg ccgcccgctg
     3181 cgcatcgcca acgacgccaa ctgcttcgcc ctgtccgagg cggtggacgg cgcggcggcg
     3241 ggggcggaga cggtgttcgg ggtgatcctc ggcaccggcg taggcggcgg catcgtggtg
     3301 cgcgggcagg cgctcaccgg cgccaacgcg attgccgggg aatggggcca caatccgctg
     3361 ccgggcggcg ctgcgccagg gcccgcctgc tactgcgggc gttcgggctg cgtggagacc
     3421 tggctgtccg ggccggggct ggcggctgac cacctgcggg tgacgggccg gacctgcacg
     3481 ccggaggccc tggtcgcggg cgcggcggcg ggcgacgcgg acagcatcgc gaccctcgac
     3541 cgctacgcgg cacgcctcgc ccgcgccctg gcgacggtgg tgaatctgct cgatccggac
     3601 gtcatcgtgc tgggcggcgg gctctccaat ctggcgatgc tgtacgaggc ggtgccgcgg
     3661 ctgtgggtgc cccacgtgtt ctcggacgcc atcgccacgc gcctgtgccg ggcggttcat
     3721 ggggattcgt cgggtgtgcg cggggccgcc tggctgtggc cggattgaga caaatccccg
     3781 cttctgtcgc acaatgccgg atcatccagc cgccttgcgg tacatcgccc cctgaatggt
     3841 gtttgacctg tgcccgtctg cccgcccgac ctgatgtcca cccccctcgc cgtgtttgcc
     3901 ggtggattgc gtgccctgcc gccggaaggc cagcagaccg gcatgttcaa ggcgccggtc
     3961 gtcgggccgg tggcggtcgg cgtcgacggc ctggcgggtg accggcaggc cgaccgccgg
     4021 gtccatggtg gccccgagaa ggccgtgcat tattttccgg ccgagcatta cgctcacctg
     4081 accacgcggg tgcccgccct cgccgacgcc ctgcggcccg gcgtgctcgg cgaaaacctg
     4141 tcggcccggg gtctgaccga agacacggtg tgcatcggcg acgtgttcgc ggcggggagc
     4201 tgccgcctgc aggtgagcca gccgcgcaat ccgtgctgga agatcaacca ccgcctcgac
     4261 cacgaccgca tgtcgcgcat catcgtcgac gacggctgca cgggctggta cttccgcgtc
     4321 ctcgcgcccg gcgtcctgac ggcgggtgag gtgctgcgcc tggtggagcg tccggcgccg
     4381 ggcatgaccc tcgcccgcct ggcggccgtc aagggcgcgc accgtccgtc ccccgacgag
     4441 ctgctcgccg tggccgcagc gcccgggctg gcaccggacg tcgcccgtcg tctccaggcg
     4501 cggggcgcat ggttgaccaa tagtggcgtg ccaccgtgct gagtgccctt gactccctgt
     4561 acagctcggc tagaaagaag cgtcatccca ccttcacatt cgggccctcc accgatgaac
     4621 ccgtcccttg agcctccgcg ccatcgcgcc ttctgtacgg ccgagtttgc cggccgtttc
     4681 tttctcgccc agctggatgt gagtcagcgc atgggcaacc tgtatgccct gctgggcatg
     4741 gggctgacgg attgcgccct gcgcagtcac gtcctgcggg ccacccacga tgcggcatac
     4801 acggtggacc tgaacgaact ggacagccgc aaggccctcc tgctggaggc cagtctggaa
     4861 ggcttcgcca ccatcctgcg caattttgcc gaggaggtgg cggcggccca cgtgcgctgt
     4921 ctggagcagg ccctcggacg ggtgccctcg ggcgccccgt cggcgctccc gccggccttg
     4981 ctgcccttcc tgcagcccga tttcgccggc ttcggccgcg ccttcaatga ggccctgcgc
     5041 gaacgggccg aggggcgcgg ctgaaggggg cggccccgac cttgaggttt gcccgcattg
     5101 acaggggcac gcgggcggcg gataagctct ccggtccggc aggggcgtga ccctgcgccg
     5161 gaccgcattc ctccgctccg taccgtccat gactcctgtc ccgtccccgc ccgcaccgcc
     5221 tgtcgcaccg ccgtcggcgc ccttcctggt cagccggatc ttcctgccct tcgccctcgg
     5281 ctactacctg tcctacctgc tgcgcaccgt caacgcggtg atctcgccgg acctgacccg
     5341 cgagttgggc ctcaatgccg ccgatctggg cctgctcacc agcacctact tcttcgcctt
     5401 cgggctggcc cagatcccgg tgggcatcgc cctggaccgc ttcgggccgc gtcgcgtcga
     5461 atccgccctg ctgctgatca ccgccctggg ggcgacgctg ttcgccctcg gcgaggatct
     5521 gccggtgctg gccggcgccc gcgggctgat cggcttcggc gtgtcggcct gcctgatggg
     5581 cggcctgaag ggcttcaccc tgtggttccc gcgggagcgc ctggcctccc tcaccggcta
     5641 catcatgtcc ggcggcgccc tcggcgcggt gacggcctcg gcaccgctgg aggcggccct
     5701 gcccttcatc ggctggcgtg gcgccttctg ggccgtggcg gtgctggcgg cggggattgc
     5761 cgcgctgatc ttctttgcca tgccggaaaa atcctccggc cacggtggtg gcgatctgca
     5821 ggcggccctg cgcggcgtga aggaggtgct ctccgcacgg cgtttctgga gcttttccgg
     5881 gcaggcagcc ttcttcaccg gcggcttcat ggccctgcag gggctgtggg cggtgccctg
     5941 gctgatgcag gtgaatggct attcccgcgc ggtggcggcg gatcacctgt tctggctcaa
     6001 cctgggcatg ctcgccgggc agctgtccat cggcgcctgg gcgacgcgcc tggcggcccg
     6061 cggcatcacg cccctgcgcc tgatgcaggg cggcctgttc ctgaccatgc tcgtcgaggc
     6121 gctgatcatc gcccgcctcg gcccgaccca gcccctgtgg tgcctgctgg gcatcgtctc
     6181 ggccaccggc gcgcagatgt acggcgtggt ggcggggctg ttcccgctgc atctgtccgg
     6241 gcgggtcact accagcatca acctgatggc ctttgtcggc gcctttgcgg tgcagtgggg
     6301 cctgggcggg ctgctggacg gcctgcgcgc cggcggtatg agcgcggcca cgtcgctgga
     6361 ggtggccttc ctgatcctgc tggcggccca ggcggccagc ttcgtgcccc tgtcacgggc
     6421 cgggcgttac acgccggtcc actgattccg cccgacccga tcgaccctgt ggggtcttgc
     6481 ggtccggagg taagcgttgt catgatgggg gcgggcggac ggtccgccct tgtcctgcga
     6541 gaagcccatg tcgtcgcgtc aactgtatgt ccatcgttat cccgccgccc atcccgaagg
     6601 tcggccgacc ctgctgttcg tccatggcgc ctattccgat ggccgcctgt gggtggagca
     6661 cttcatcccg tggttccgcg aagccggcta cacctgcctg gcggtggatc tgtcgggtca
     6721 tgggcgcagc gaagggcggg acgacctcga cagcctgggt ctggacgact atgtggatga
     6781 cgtggcccag gtcgttgcca cgctgcgcgc gccgccggtg ctgatcgccc attccatggg
     6841 ttccctggtg gcccagcgct atgcgcagcg cgcgccgctg gccgggatgg ccctgctggc
     6901 gccggtgcct cccaccggga cgggcggttc ggctggccgg ctggcgctga tgctgcccga
     6961 gtttttcgag gagctgcccc gcgccatcgc cggttcggcc ggcccgcgga ccctggaggt
     7021 catggccagg gtgtactttt cgccggagat gcctgcccgc gagacggcgc actacctgcc
     7081 cctgctgcag gcggaatccg aacgggccgt gctggaaatg gtcacgctgc ccctgcgcgg
     7141 tctgtcctgc cgtccgcggc tgccggtgct ggtgatgggc ggcagccagg acgcggtgtt
     7201 cccggcgtcg atgctgtgct tcaccgcgct gccctggcgc gcgcaacagg acgtggtgtc
     7261 cggtgccgga cacatgctga tgctggatcc ccaatgggaa gacgccgcca cgcggatcgc
     7321 cgcgtggctg gagacgctgc ccgcggcgac gtgacgccgc gctgccgggg cgcatgcggg
     7381 ccgctcaggc ccggtccacc atcacgtagc gcttggtgac ggtttccagc acctcgaaca
     7441 cgcccttgaa gccggcgggg atcacgcagg cgtcgccggg gccgaagtcc cgcgcgtggc
     7501 cggcgtcgtc gatgaggcgc aggcgtccgc tcagcacctg gaagaactcg tgccggccgg
     7561 catggaacgc gatgcgccag gcgcccggct cgcaggtcca ctcgccgatg gacagggcgc
     7621 cgtcctcggc gctgtagcgt tcggcggtga ggcgccgggg cggcgggccg agggcccgtt
     7681 ccggggccgg gcgatcttcg ctggccgcgg gcgcagggtc ggcaaagtgg atcagggcgg
     7741 gctgcggggg ggcgatcggc gtgggcatgg gcagaacgag aggtcggtca tcagaacaac
     7801 tccacgtcgt cgcccttgtc cttcaggccg gccttgtact tttccagggc atccttgacc
     7861 ggcatgccct gcatgaccgc gttgaacatg tccacctctt cgcgggtgga gtatttggcg
     7921 cggatctttt cctgcagggc cacccactcg aaggggttgt acagcttgcg ctgatccact
     7981 accaggccgg acagggcctc caggctgtgg gagacgtggg cgagacgctg ggtgagctta
     8041 tcgtagaact ggaaggcgat gatggagcgc tggatgcgcc cggagacctc gccggcgtgg
     8101 ccgatcaggt tgtccttgag gttctgggtg gcgggcgtgt cgggcagctg ttccagtgcg
     8161 ccggcgatca tgcgcatgta gccggccatg gaggtgaagg agtcggtgag cacctccacc
     8221 gagctgtggc cttccttcat ggccgcctcg atctggccga cggccagttc cagcatcagg
     8281 acggtttcgc gcacctggct ccagtccagg tcgggggtgt gggcgctggt gccgcggcgg
     8341 gtatcgtgct cagacatggg ggctccgttt tccggtgtga atggcatacc ggcttttcgg
     8401 cgcggccccg aaaagcttta gggcgtcggc ggcgtcccgg cgaacaccgg ctccggctcc
     8461 agatccacgc cgaagcgggc cgccacgtcg gcgcgcacgg cccgggcgac ggcgatcacg
     8521 tccgcccccg tggcgccgcc gtgattgacc aggaccaggg cctggcggtc atagcagccc
     8581 accgggccga ggcggcggcc cttccagccg gcctggtcga tgagccagcc ggcggccagc
     8641 ttctcgcgcc cgtcggcctg ggggtaatgg ggcaggccgg ggtgggcggc ggcgaggcgg
     8701 gcgtaggtgg cggcgtccac caccgggttc ttgaagaagc tgcccgcgtt gccgatctcg
     8761 gccggatcgg gcagcttgcg gcggcggatg gcgatgaccg cgtcggagat ctgcagcggt
     8821 gtgggctgca cgaggccgcg ggcggccagc tcggcggcca catcggcgta gccggtgacg
     8881 ggcttccacg ccttgggcag gcggaaggtg accgcggcga tcaggtaccg cccggcgccg
     8941 gcctgcttga agaagctgtc ccggtagccg aagccacagt cgcgcttgtc gaagcccacg
     9001 gtcttgccgg tggccaggtc gtaggcgcgc aggctgtaga agtgctcggc catctccagg
     9061 ccgtaggcgc cgatgttctg caccggcgcg gcgcccaccg tgccgggaat cagggacagg
     9121 ttctcgagtc ccggccagcc ctgttccagg gtccagcgca cgaagtcgtg ccaggattcg
     9181 ccgccgccgg cgcgcacgta gtaggcgtcg gcgtcctcct gcaccttgct gcggccgtgg
     9241 aaggccacgt gcagcaccag cccggcaaaa tccccggcca gcaccaggtt ggagccgccc
     9301 cccagcacca gcctgggcag gcccgccacg cgggggtcgt cgagcacggc gcgcaggccg
     9361 tccacgtggt ccacgcgggc gtaccaggcg gcgcgggcgg gcaggcgcag ggtgttgaag
     9421 gcgtcgaggg cgacgtcctg ctggagcaga tcggcggagg cggtcatggc ggcggggcgg
     9481 cttattggcg aatctcggcg agcaggggcg gcggcggcag gccgggcgtg cggcggcagg
     9541 ggttgatgtc gagcccgccg cggcgggtgt agcgcgccca cacgcacagc tcggcggggc
     9601 ggcagcgcgc caggatgtcg gtgaacaccc gctccacgca ttgctcgtgg aactcgttgt
     9661 ggcggcggaa ggacacgatg tagcgcagca gcccctcccg gtcgatgggc gcgccccggt
     9721 agcgcacgac cagcatcgcc cagtccggct ggccggtgac caggcagttg gacttgagca
     9781 ggtgggaata cagggtctcg ctcactgcgg gcgcggcggt gtcggcgcgg agcagctcgg
     9841 gcgccggtgt gtagcggtcg atggcgatgt cctggctgtc cagcagcacg ccctctgggt
     9901 agcccgtgtc ccgctgcggg cgggcggaca ggggctccac cgtcaccgcc accggcgcac
     9961 cggccgcggc agaaagatcg gccgcgagca tgcccgccag cgcgtcggcg ctcgccaccg
    10021 gcgtctggtt gaaggcattg agatacagct tgagggactt ggattccacc aggcagggcg
    10081 aatcggccgg aaacacgaag cgcgccacgg ccacctccgg cttgccgcgg gggttcagcc
    10141 acgagagctc gtaggcgttc cacacatcct cgccgacgaa gggtaggctg ccggcggcga
    10201 ggcccagctc gtcacgcttg ccctggcgca ggatcggaaa gagcaggctc gggtcgtagt
    10261 gctcggcgta ggcgacgggc ttgcccaggg gcgaggcgtc gggatgggcg gggggcggca
    10321 gtgtgctcat ggccgccatt ttatcggctg gggcgccgcg gcggggcgcc gtcgtccgac
    10381 gcggggccgg gcgcctgggt gcgcccgggg cgtgccggtc gccccttgaa ccccgccccg
    10441 cccgctccca caatggcgcc gtcgcgcccc acccgatctg cccatgaacg ccctgcccat
    10501 ccgttacgtg gacccggtct atcgcccgcc cagcgaagcc cagtccctga tcctgcccgt
    10561 caccgacggc tgctcctgga accactgcac cttctgcgag atgtacaccg acccgcagaa
    10621 agccttccgc gcccgcagcg aggacgaagt gctggccagc atccgcgcca ccggcgagcg
    10681 ctacggcgac cagatgcgcc gcgtcttcct cgccgacggc gacgccctgg tgctgcccac
    10741 ccgccgcctg ctgcgcatcc tggaagccat ccgccaacac ctgcccgccg tgcaccgggt
    10801 gtccagctac tgcctcgccc gcaacctgcg ccggaagact gtcgccgagc tgcgcgccct
    10861 gcgcgaggcc ggcctggccc tcgcctatct gggcgccgaa tccggcgacg acaccgtgct
    10921 ccagcgcgtg aacaagggcg aaaccttcga ctccacccgc gccgccctcg acctgctcgg
    10981 cgaagccggc atcggccgct ccgtgatgat cctcaacggc ctcggcggcc cgctgttcag
    11041 ccgcgcccac gccgaacact ccgcgcgcct cgccaacgcc acccagccgg aatacctgtc
    11101 caccctggtg gtcagctttc cccagggcga agcccgcttc cgtgccggct tccccgaatg
    11161 ggcgccgctg gacccgcccg ccctgttccg cgagatggag accttcctca ccgccctgga
    11221 cctgcaccgc accgtcttcc gctccgacca cgccagcaac tggctggtcc tcaagggcac
    11281 cctcggcgcc gacaaggacc gcctcctcgc ccaggtccgc gccgcgctgg aacggcccga
    11341 cgcggccgcg ctgcggccgg cgtgggcgcg gggg
//
LOCUS       DAOQPA010000109        11236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2978_length_11236_cov_18.018245, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000109 DAOQPA010000000
VERSION     DAOQPA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2978_length_11236_cov_18.018245"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1203
                     /locus_tag="PKX01_13615"
                     /pseudo
     CDS             73..1203
                     /locus_tag="PKX01_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
     gene            1336..3039
                     /locus_tag="PKX01_13620"
     CDS             1336..3039
                     /locus_tag="PKX01_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMZ77215.1"
                     /translation="MARLFSRTLRRLGAACSVLAPLAGLLAGGGLAQAQTSYSSGYWY
                     GNASPVSVLTHHNDGWRTGWNYAETTLTPSNVAATGGSKLFGLQGSVDLDGLVEVQPL
                     VVPNAYVYGDPNAGRHDVVYVATANNTVYAIDPTTRKVLRSRNLGTPMSAFPGCEDND
                     KIIGVNSTPVIDPSTNSLYLIAHVRVGSGSEMHLHRLSLSSLKDIVAPVTIAAKAPLT
                     NGSTATFDANYQRQRTALLLANGRVMAAFTSHCDIRADVARGWVMQWDAQTLDKARSY
                     VDFSGRKSVFLTDTQATGPSNFFLATSWMSGAGPAVDEWGNVYFATGNTDPGALRSDG
                     TGYVEDGVTNFGQSVVKLSPWDSRVLSYFTPANVMQLDNDDLDLGAGGVLLVPSYTDR
                     LALQAGKDGVLRLMNRDSLGGHTPGGPDKVLAATQIGPCWCTPSYFNDGTPKVVSSGG
                     TTLGVYAIRTKPYGLVKQAGVEINSGQFGGFFTSVSSNGAQNAIIWAVGRPQTIPGDL
                     YLHAYSAKPVNGSLPLLYTTTAGTWNSQGNANTVPTVANGRVYVAGYKKLSIYGLGGQ
                     R"
     gene            3056..3367
                     /locus_tag="PKX01_13625"
     CDS             3056..3367
                     /locus_tag="PKX01_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77216.1"
                     /translation="MKTTRILILAAALGGVTAPVLAEAPNAVTGVVRSIAGATLQVEL
                     RGGTALQVEQSDALAAHQVAPGIKVGTAVHATGTRSGNLLRAETVTRAKPAPGTWSPD
                     R"
     gene            complement(3566..4363)
                     /locus_tag="PKX01_13630"
     CDS             complement(3566..4363)
                     /locus_tag="PKX01_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77217.1"
                     /translation="MYVSDLVSHLQTLIACSALDFVFQPIFDLRRREITGYEALMRGP
                     DDSPLNTPAQLLQVAREAGLELALERLASRLALEAFAAVELPGSLFLNLSLAALIDQA
                     RTDGVVALAGRAGLPPTRLVVEPLPDAPATGCAATACASLDAQGVRIAVHGALPPQER
                     AGFRPAFLKLGPAAFHRIHADPLRRLDLASVLAEAARRGVQVVAEGVEDARELATLFD
                     LGCDCAQGHLLGRPAADPLRRPSRGVDGLLQARAILQPRPADATLPV"
     gene            complement(4495..5379)
                     /locus_tag="PKX01_13635"
     CDS             complement(4495..5379)
                     /locus_tag="PKX01_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ77218.1"
                     /translation="MNWDDLRIAAAIGRAGTLAAAARRLGIDQTTVARRLRALEDALG
                     TPLFERGEGGWQPTAPGSEVLARAWRMEEDAADITRIADAGARSVSGRVHLTSVGPIV
                     ADYLAPRLASLMSTYPALDVDLIARNDNLSLARREADIALRLGRPADGDFLIRKLADV
                     GFAVYAPADTPIDPVAWVAYGEDLDHTPEMRWLAARLNGPAGNTAARIRLRCDSLRGL
                     TRAIAAGIGCGVLPCFIGDACSDLRRVPTDALPVRELWQLVHRDARRLERVDAVSTWL
                     AERFAADADLFRGLHPPA"
     gene            5501..6100
                     /locus_tag="PKX01_13640"
     CDS             5501..6100
                     /locus_tag="PKX01_13640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HMZ77219.1"
                     /translation="MADAFTLRALMGKPVTPTRLSESVLILIDAQNAYRHGPLTLSGI
                     EPALASCARLLARARALGAPVIHIQHDAGPGTPFDPLSESGAFIDVVAPRPGEAVVVK
                     HFPNAFVGTDLEARLQALGARNLVIAGFMTHNCVNSTARGAYNLGYRFTVPGNATATR
                     ALPAIGGGQLDAATLQAALLVSLTDVFGVVVADEAEIPD"
     gene            complement(6161..6598)
                     /locus_tag="PKX01_13645"
     CDS             complement(6161..6598)
                     /locus_tag="PKX01_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ77220.1"
                     /translation="MPTRPVSQVVSRRSFLKAPGTTSAAEAARMMREQHKSTVTVVNA
                     TGRVEGICSERDLVQKVMAAGRDPAITLLAEIMTPNPQTIPADKPFCHALHIMFDGGF
                     RHLPVVDEAGRPIGIVSVRDALGLEMIQFGHEMEQRDTLTEIL"
     gene            6824..8758
                     /locus_tag="PKX01_13650"
     CDS             6824..8758
                     /locus_tag="PKX01_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ77221.1"
                     /translation="MTAPPVAASARRVLSLIPPMTQLNTPYPSTAYLTGFLRSRGVDA
                     VQDDLALKLVLELFSPDGLEAIRARAEALPARRQSPAVQGFLAQFARYRATVGPAVAY
                     LQGRDPTVAHRIASRQFLPEGGRFAALDQYVDPDGGDPLAWAFGALGTADKARHLATL
                     YLNDLADVLRDAVDPRFEFVRYAESLARSQPGFEPLARALAAPPTLVDEVLESLAAEA
                     LTRHAPQVVLLSVPFPGSVYGAFRIAQAIKARAPQVVTVLGGGFANTELRELSEPRVF
                     DFFDYVTLDDGERPLLALLEHLDGRRGPSRLVRTYRRDEAGTVRYEHWVEPDVPFAEV
                     GTPTWDGLPLDRYLSVLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLDYIGR
                     YDGAAAALLADRIDAIVTETGESGFHFVDEAAPPKALRALADELLQRGRAISWWGNIR
                     FEKSFTPELCERLAESGCIAVSGGLEVASDRLLKLMKKGVSVDQVARVTRAFSDAGIL
                     VHAYLMYGFPTQTVQDTVDALEYVRQLFDAGCIQSGFFHRFACTVHSPVGLNPAEYGV
                     TLKALPPVSFARNDVEFVDPSGVDHDALGRALNKALYNYMHGIGLDEDVRAWFEGRVP
                     RTRVPRRFVERALGVAPARA"
     gene            complement(8765..>11236)
                     /locus_tag="PKX01_13655"
     CDS             complement(8765..>11236)
                     /locus_tag="PKX01_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77222.1"
                     /translation="WRDFVTPMMARNPGTQNFGWSPLVTDAQRDAFEARVRAAGRPDF
                     RILGRNVAGQTFPAARKAEYLPILFVEPLAENRSVLGLDPLVLDRTASAVQRSRKSGL
                     PIASESIRLVQETGEQRGIVVYQAVLAGSPPHMTGLVSAVFRMDDAMQAILGHRRTGE
                     LDVCLSEPRTGASERLYGPAGCEQAGWAGKRLNWETPLTFAGRTWLLRVGATSAYMNT
                     VGNWGLWLMLALGLLSTGILGAFLLLTSGRTRRVESLVDERTRQLAAATDTLRRQQQE
                     LAQAQRIARLGSWETLPGSDELRCSDELMAMLTLDGSTPIRTRQLIERLHPDDRPRFL
                     HALEQVDAMPHRIDLDCRLARDGTPMEILHFRLESEGHGPALRIRGTAQDVTSARAAE
                     ARIARLAHFDVLTGLPNRTQWNERAHSELRAAARHGDILAVLFLDLDHFKAVNDSLGH
                     PVGDQLLSAVARRLSGCLRENDFLARLGGDEFVVLLPRLSHREDAALVARKLIAALEH
                     PVVMGAHELTVSVSIGIALHPDDGSDVDTLLKHADLAMYRAKDQGRNAYHYFLPDMDV
                     RAMERLLLETALRRAVERDELRLHYQPQLRTRDSRVTGCEALVRWQHPELGLLPPAQF
                     IGVAEASGIIHALGAWVLEHACRQQVIWATAGRPLIMAVNISALQFRQAHFYGTVKRI
                     LEDTGIDPAHLELELTESALMQLTPEVLERMHALRALGVNLALDDFGTGYSSLSHLKR
                     LPIRRLKLDRSFVEALPADLEDAAIASATLSLAQDLGMEVVAEGVESPSQVDFLTRRG
                     CPLLQGFLFSEPLPAAEFDAWLLAR"
BASE COUNT         1638 a         3836 c         4031 g         1731 t
ORIGIN      
        1 ggccctcgcc ggggcgcatg aaggtggtct tcatctcgat ggtcaccacg cccggaccgc
       61 cgccgccagg cccgccgcga ggacatgccc gacggccgcg acgccctgat cctgcccttc
      121 cgcgccgaga gcctgctgtc ctttgccgac tacgtgaagg tgaaccgcgc ccatgacgac
      181 gatgcggacg acgatcccgg ccgcgtcgcc gcggagatgg acaccgtgtc ggtggcccag
      241 gacggtcagg acacggcgtc caaggtgcgc ttcgacctgg acctgccctc cgccgcccag
      301 gacgacatcc cgctgacgac cggcatcctg ctgcccgaat gggactggaa gacccagcag
      361 ttgcggccgg acctgtgcag cctgagcgtg ctcgaccccc gtgattccga accctgtgcc
      421 ctgccgccgc ggctgatccg cccggcgcga cgcctgcgtc gccagttcga ggccctcacg
      481 ccggcgcggc gctggctcaa gaaccagccc gacggcagcg acctggacat cgacgcctgc
      541 gtgcgcgcct ttgccgaccg catgaccggc cattcggtca gcgccagcgg cggctacctg
      601 gcctgcgagc gccgcgagcg cgacctgtgc tgcctggtgc tggcggatct gtccctgtcc
      661 accgacgcct gggtgagcaa cgaggcgcgg gtcatcgacg tgatccgcga cagcctgtgg
      721 ttgttcaccg aggccctcgc cgccaccggc gatcccttcg gcctctacgg tttttcgtcg
      781 cgcaagcggg agcacatccg cttccaccgc atcaaggatt tcgacgagcg gcccggtgac
      841 cgggtgcgcg ggcgcattgc ggcgatcaag ccgggcttct acacgcgcat gggcgcggcc
      901 atccgctacg ccacccggct cctcgcccgg cggccggaat ccgtgcgcct gctgctgatc
      961 ctgtctgacg gcaagcccaa tgatgtggat cattacgagg gccgctacgg catcgaggat
     1021 acgcgcatga gcctgcgcgc cgcccgcgag gagggcgtgc ggcccttctg cgtcaccatc
     1081 gaccgggaag gggcgggcta cctgccccac ctgtttggcc cggccggcta caccatcctg
     1141 cgcaagccgg cggatctgcc ggcccgcctg cccctgctct acgcccagct cacgggcgcc
     1201 tgatggcgcc gggaatgccg gtgtgtgaca ggtggcacgg gcggtacgtg tttctggcaa
     1261 atctgacgat ttgtaactgg ttgcgacgcg cgggccgtcc tagcatgggc tattgccatt
     1321 tttatggaat cagccatggc ccgtttgttc tcacgcaccc tgcgccgcct cggcgcggcc
     1381 tgttccgtcc tcgctcccct ggccggactc ctcgccggcg gcggcctcgc ccaggcccag
     1441 acgtcctatt ccagtgggta ctggtacggg aacgccagtc ccgtatcggt gctgacccac
     1501 cacaatgacg gctggcgcac cggctggaat tacgccgaga ccaccctcac cccgtccaac
     1561 gtggcagcca cggggggcag caagctgttc ggcctgcagg gcagcgtgga tctggacggc
     1621 ctggtggagg tgcagcccct ggtcgtgccc aacgcctatg tgtacggcga tccgaatgcc
     1681 ggccgccatg acgtggtgta cgtggccacc gccaacaaca ccgtctatgc catcgacccg
     1741 accacccgca aggtgctcag gagccgcaac ctgggcacgc ccatgtcggc gttccccggt
     1801 tgcgaggaca acgacaagat catcggcgtg aacagcacgc cggtcatcga cccgtccacc
     1861 aattccctgt acctgattgc ccatgtcagg gtcggcagcg gctccgagat gcacctgcac
     1921 cgcctgtcgc tgagctccct caaggacatc gtggcgcctg taaccatcgc ggccaaggcc
     1981 cccctcacca acggcagcac cgccaccttc gacgccaact atcagcgcca gcgcaccgcc
     2041 ctgctgctgg ccaatggccg cgtaatggcc gccttcacct cccactgtga catccgcgcc
     2101 gacgtggccc gcggctgggt gatgcagtgg gacgcccaga ccctcgacaa ggcccgcagc
     2161 tacgtggact tcagcggccg caagtccgtg ttcctgaccg acacccaggc taccggcccg
     2221 agcaacttct tcctggcgac cagctggatg tccggtgccg gcccggccgt ggatgaatgg
     2281 ggcaacgtgt atttcgccac cggcaacacc gatccgggcg ccctgcgcag cgacggcacc
     2341 ggctacgtgg aggatggcgt caccaacttc gggcagagcg tggtcaagct ttctccgtgg
     2401 gacagccgcg tgctgagcta cttcaccccg gccaatgtga tgcagttgga caacgatgac
     2461 ctggacctgg gcgccggtgg cgtcctcctc gtgcccagct acaccgaccg cctcgccctg
     2521 caggccggca aggacggcgt gctgcgcctg atgaaccggg acagcctggg cggccataca
     2581 ccgggcggac cggacaaggt gctcgccgcc acgcagatcg ggccgtgctg gtgcaccccg
     2641 tcctacttca acgacggcac gcccaaggtg gtcagcagcg gcggtaccac gctcggcgtg
     2701 tatgccatca ggaccaagcc ctatggcctc gtgaaacagg ccggcgtgga gatcaattcc
     2761 ggccagttcg gcggcttttt caccagtgtg tcgtccaacg gcgcccagaa cgccatcatc
     2821 tgggcggtgg gccgacccca gaccattccg ggcgatctct atctgcacgc ctattccgcc
     2881 aagccggtga acggcagcct gccgctgctg tacaccacca ccgccggcac ctggaacagc
     2941 cagggcaacg ccaacaccgt gcccaccgtg gccaatggcc gtgtgtatgt ggccggctac
     3001 aagaaactgt ccatctacgg cctgggtggc cagcgctgat cgcgaggaag attccatgaa
     3061 gaccacacgc atcctgatcc tcgccgccgc cctgggcggt gtcaccgccc cggtcctcgc
     3121 cgaggcgccc aatgccgtca ctggcgtggt ccgcagcatt gccggcgcca ccctgcaggt
     3181 ggaactgcgc ggcggcacgg ccctgcaggt cgagcagagc gacgccctgg cggcccacca
     3241 ggtcgcgccg ggcatcaagg ttggcaccgc cgtgcatgcg acgggtaccc gcagcggcaa
     3301 cctgctgcgc gccgagaccg tgaccagggc caagccggcg ccagggacct ggtcgccgga
     3361 tcgctgattc ctccggtccg gaccgcggcg cacggtccgg atccggcacc tgcgtaccag
     3421 caaggccaac gacccccgac ggcgatcact cccgtgccgt cggcgggctc gggggttggc
     3481 cgacgtcccc gcaatccccg cctgggcgat gtcctggcgg ggattggcgt ttaccggcag
     3541 cgggctcgag cggaccgacc ggctatcaga caggcaaggt ggcgtccgca gggcggggct
     3601 gcaggatcgc gcgggcctgg agcaggccgt ccacgccgcg ggacgggcgg cgcagggggt
     3661 cggccgccgg gcggccgagc aggtggccct gggcacagtc gcagcccaga tcgaagagcg
     3721 tggccagctc gcgggcgtct tccacgccct ccgcgaccac ctggacgccg cgccgggcag
     3781 cctcggcgag aacgctggcc agatcgaggc ggcgcagggg atcggcgtgg atgcgatgga
     3841 aggcggcagg tccgagcttg aggaaggctg gccggaatcc cgcccgctcc tgaggtggca
     3901 gcgcgccgtg caccgcgatg cgcacgccct gggcgtccag ggacgcgcag gccgtcgccg
     3961 cacacccggt tgcaggtgca tcgggcagag gctcgacgac cagccgggtg ggcggcaggc
     4021 cggcccggcc ggcgagggcc accacgccgt cggtgcgagc ctggtcgatg agggccgcaa
     4081 ggctcaggtt gaggaacagg ctgccgggca gctccacggc ggcaaaggct tccagcgcga
     4141 ggcggctggc gaggcgctcc agggccagct ccaggccggc ttcccgtgcc acctggagga
     4201 gctgggccgg ggtgttgagc ggcgaatcgt cggggccccg catcagggct tcgtagccgg
     4261 tgatctcccg gcggcgcaga tcgaagatcg gctggaagac gaagtccagc gccgagcagg
     4321 caatgagggt ctggagatgg ctgacgagat cgctgacgta catggtgaag cagggcgcgg
     4381 gaaatggcgg ggcaataccg tcattctcga cagactttca tccggattga atgaattttt
     4441 tctgacggct gccagcgttg cggccgttgg caccggatcc ggcgggcccc tgcgtcaggc
     4501 gggtgggtgc aggccgcgga acaggtcggc gtcggcggca aagcgttcgg ccagccaggt
     4561 gctgaccgcg tcgacgcgtt ccaggcggcg ggcatcgcga tggacgagct gccacagttc
     4621 ccgcaccggg agcgcgtccg tgggcacgcg ccgcagatcg ctgcaggcat cgccgatgaa
     4681 gcacggcagg acgccacagc cgatgccggc ggcgatggcg cgggtcaggc cgcgcaggct
     4741 gtcgcagcgc aggcggatgc gggccgcggt gttgcccgcc gggccgttca ggcgggccgc
     4801 gagccagcgc atctcgggcg tgtggtcgag gtcctcgccg taggccaccc acgcgacggg
     4861 gtcgatgggc gtgtcggcag gcgcatagac cgcgaacccc acgtccgcca gcttgcggat
     4921 caggaaatcc ccgtccgccg gtcgccccag gcgcagggcg atgtcggcct cgcgacgggc
     4981 caggctgagg ttgtcgttgc gggcgatgag gtccacgtcg agcgccgggt aggtgctcat
     5041 caggctcgcc aggcgcggcg cgaggtagtc ggcgacgatg gggcccaccg aggtgaggtg
     5101 cacccggccg ctcacgctgc gggcgccggc atcggcgatg cgggtgatgt cggcggcgtc
     5161 ctcttccatg cgccaggccc gcgccaggac ctcgctgccc ggggcggtgg gctgccagcc
     5221 gccctcgccc cgttcgaaca ggggggtacc gagggcgtcc tcgagggccc gcaggcggcg
     5281 ggcgacggtg gtctggtcga tgccgagacg gcgcgccgcg gcggccaggg tgcccgcccg
     5341 gccgatggcc gcggcgatgc gcagatcgtc ccagttcatg gtgggggctc cgggatacaa
     5401 agctgcatta ttgcaggcct gatctgcaag gatgtcgatt gttgcaggta atacggggcg
     5461 ctacgctggc gccgtcttca catcctgcaa ggagcttgcg atggccgatg ccttcacgtt
     5521 acgtgccctg atgggcaaac ccgtcacgcc gacccgcctg tccgaatcgg tgctgatcct
     5581 gatcgatgcc cagaacgcct accgccatgg accgctgacc ctcagcggca tcgagccggc
     5641 cctcgcgtcc tgtgcccgcc tgctggcccg cgcgcgggcg ctgggcgcac cggtgatcca
     5701 catccagcat gacgccggtc cgggcacccc tttcgatccg ctgagcgaaa gcggcgcctt
     5761 tatcgacgtc gtcgcgccgc gacccggcga ggctgtcgtc gtgaagcact ttcccaatgc
     5821 ctttgtgggc accgacctgg aggcgcgcct tcaggcgttg ggcgcccgga atctggtcat
     5881 cgcgggcttc atgacccaca actgcgtcaa ttccaccgcc cgcggcgcct acaacctggg
     5941 ctatcgcttc acggtgccgg gcaacgcgac ggcgacccgc gcgctgccgg cgatcggcgg
     6001 tggacaactg gacgcggcga ccctgcaggc cgccttgctg gtgtccttga cggacgtgtt
     6061 cggcgtggtg gtggccgacg aggccgagat tcccgactga ggagtcccgg ggcgcccggc
     6121 tcgcccttgc ggggggacgg ttcgggcgcc gacgccacgc tcagaggatc tcggtgaggg
     6181 tgtcgcgctg ctccatttcg tggccgaact ggatcatctc cagcccgagg gcgtcgcgca
     6241 ccgagacgat gccgatgggc cgcccggcct catccaccac cggcaggtgg cggaagcccc
     6301 cgtcgaacat gatgtggagc gcgtggcaga agggcttgtc tgccgggatg gtctggggat
     6361 tcggcgtcat gatctcggcc agcagcgtga tggcggggtc gcgccccgcg gccatgacct
     6421 tctggaccag gtcgcgttcg gagcagatgc cctcgacgcg cccggtggcg ttgacgacgg
     6481 tgaccgtgct cttgtgctgc tcccgcatca tgcgggcagc ctcggcggca gaggtggtgc
     6541 cgggggcctt gagaaagctg cggcggctca cgacctggga tacgggacga gtcggcatgg
     6601 gagtctccgg gggtgtggga gggattcgtt tgtgatgcgc gccgggttgc gggttccatt
     6661 ggatgttgca atgatttccg cggcttccgg tggcgcattc aattggacag gccggcggtc
     6721 ggcaccatgg gcgggcgggg ggctatcatg tcgcgctgtt tctccggaga agtgtcatgc
     6781 cagtctggcc cgcgggcgtc ggcccgtgtc gcaggagccg tccatgacgg cgccgcccgt
     6841 cgccgcatcg gcccggcgcg tgttgtccct catcccgccg atgacccagc tcaacacgcc
     6901 ctacccctcc acggcctacc tcaccggctt cctgcgcagc cgcggcgtgg acgcggtgca
     6961 ggacgacctg gccctcaagc tggtgctgga gctgttctcg ccagacgggc tggaggcgat
     7021 ccgggcccgc gccgaggcct tgccggcgcg gcggcagtcg cccgcggtgc agggcttcct
     7081 ggcgcagttc gcgcgctacc gtgccaccgt cggcccggcg gtggcctatc tgcagggacg
     7141 cgatccgact gttgcccatc gcattgccag ccgccagttc ctgccggagg gcgggcgttt
     7201 tgcggccctg gaccagtacg tggacccgga cggcggcgat ccgctggcct gggcgttcgg
     7261 tgccctgggc acggcggaca aggcacgcca tctggcgacc ttgtacctca acgatctggc
     7321 ggatgtgctg cgcgatgcgg tggacccgcg cttcgagttc gtccgctacg ccgaatccct
     7381 ggcccgcagc cagcccggct tcgagcccct ggcgcgggcc cttgcggcgc cgccgacgct
     7441 ggtggacgag gtgctggaaa gcctcgccgc ggaggccctg acccgtcatg cgccccaggt
     7501 ggtgctgctg tccgtgccct ttccgggctc ggtctacggc gccttccgca tcgcccaggc
     7561 gatcaaggcc cgcgccccgc aggtggtgac ggtgctgggc ggcggctttg ccaacaccga
     7621 actgcgcgag ctgtccgaac cgcgggtctt cgacttcttc gactatgtga cgctggacga
     7681 cggcgagcga cccctcctcg ccctgctgga acacctggac ggccgccgcg ggccgtcgcg
     7741 cctggtccgc acctaccgcc gcgacgaggc cggcacggtg cgttacgagc actgggtgga
     7801 gccggacgtg ccctttgccg aggtgggcac ccccacctgg gacggcctgc ccctggaccg
     7861 ctacctgtcg gtgctggaca tgctcaaccc gatgcaccgc ctgtggtccg acgggcgctg
     7921 gaacaagctc accgtggccc acggctgtta ctggaagaag tgcagtttct gtgatgtgag
     7981 cctggactac atcgggcgct acgacggcgc cgcggccgcg ctgctggccg accgcatcga
     8041 cgccatcgtc accgagaccg gtgagagcgg cttccatttc gtggacgagg cggcgccgcc
     8101 caaggccttg cgggccctgg ccgacgagct gctgcagcgc ggtcgggcga tctcctggtg
     8161 gggcaacatc cgcttcgaga agtccttcac cccggaactg tgcgaacgcc ttgccgagag
     8221 cggctgcatc gccgtgtccg gcggcctgga ggtggcctcg gaccgcctcc tcaagctgat
     8281 gaagaagggt gtgtcggtgg atcaggtggc gcgggtgacg cgggccttca gcgacgcggg
     8341 catcctggtg cacgcctacc tgatgtacgg cttccccacc cagaccgtgc aggacacggt
     8401 ggatgcgctg gaatacgtgc gccagctctt cgacgcgggc tgcatccagt ccggcttctt
     8461 ccaccgcttt gcgtgcacgg tgcattcgcc ggtggggctc aatccggccg aatacggcgt
     8521 caccctcaag gcgctgccgc cggtcagctt cgccaggaac gacgtggaat tcgtcgaccc
     8581 gagcggcgtg gatcacgacg ccctcgggcg ggccctcaac aaggcgctgt acaactacat
     8641 gcacgggatc ggtctggatg aggacgtgcg cgcctggttc gagggccgcg tgccgcgaac
     8701 gagggtgcca cggcgcttcg tggagcgcgc cctgggcgtg gcgccggcgc gggcctgagg
     8761 ggcatcagcg ggcgagcagc caggcgtcga attccgcggc gggaagcggc tcgctgaaca
     8821 ggaagccctg gagcagcggg cagccgcggc gcgtcaggaa atccacctgg gacgggctct
     8881 ccaccccttc ggcgaccact tccatgccca gatcctgggc gagggacagg gtggccgagg
     8941 cgatggcggc gtcttccagg tcggcgggca gggcttccac gaagctgcgg tccagcttga
     9001 ggcggcggat gggcaggcgc ttgaggtggc tgaggctgga atagcccgtg ccgaagtcgt
     9061 ccagggccag gttcacgccc agggcgcgca gggcgtgcat gcgctccagc acctctggcg
     9121 tgagctgcat cagggcgctc tcggtgagct ccagttccag gtgggccggg tcaatgccgg
     9181 tgtcttcgag gatgcgcttc accgtgccgt aaaaatgggc ctggcggaac tgcagggcgg
     9241 agatattgac cgccatgatc agcggccggc cggccgtggc ccagatcacc tgctggcggc
     9301 aggcatgttc cagcacccag gcgccgaggg catggatgat gccgctggcc tcggcgacgc
     9361 cgatgaactg ggccggcggc agcaggccga gctccgggtg ctgccagcgc accagggcct
     9421 cgcagccggt caccctgctg tcgcgggtac gcagctgggg ctggtagtgc aggcgcagtt
     9481 cgtcccgttc gacggcacgg cgcagggcgg tttccagcag cagccgctcc atcgcacgca
     9541 cgtccatgtc cggcaggaag tagtggtagg cgttgcgtcc ctggtccttg gcccggtaca
     9601 tggccagatc ggcgtgcttg aggagggtgt ccacgtcgct gccgtcgtcc gggtgcaggg
     9661 cgatgccgat gctcaccgac acggtgagtt cgtgggcgcc catgaccacc gggtgttcca
     9721 gcgcggcaat cagcttgcgc gccaccaggg cggcgtcttc ccggtgggac aggcgcggca
     9781 gcaggacgac gaactcatcg ccgccgaggc gggcgaggaa gtcgttctcc cgcaggcagc
     9841 cgctcaggcg gcgggccacc gcggagagga gctggtcgcc caccggatgg cccagggagt
     9901 cgttgaccgc cttgaagtgg tccaggtcga ggaacagcac ggcgaggatg tcaccgtggc
     9961 gggcggcggc gcgcagttcg gagtgggccc gctcgttcca ctgggtgcgg ttgggcaggc
    10021 cggtgagcac gtcgaaatgg gccagccggg cgatgcgggc ttcggcggcg cgggcggatg
    10081 tgacgtcctg ggcggtcccg cggatgcgca gcgcggggcc gtggccctcg ctctcgaggc
    10141 ggaagtggag gatctccatg ggggtgccgt cccgggcgag gcggcagtcc aggtcgatgc
    10201 ggtgcggcat cgcgtccacc tgctcgaggg cgtgcaggaa gcgcgggcgg tcgtccggat
    10261 gcaggcgctc gatgagctgg cgggtgcgta tcggcgtgct cccgtcaagg gtgagcatgg
    10321 ccatcagttc gtcggagcag cgcagttcgt cgctgcccgg cagggtttcc cagctgccca
    10381 gacgggcgat gcgctgggcc tgggcgagtt cctgctgctg gcggcgcagg gtgtcggtgg
    10441 cggcagcaag ctggcgggtg cgctcgtcca ccaggctctc cacccggcgg gtgcggccgc
    10501 tggtgaggag caggaaggcg ccgaggatgc cggtgctgag caggcccagg gccagcatca
    10561 gccacagacc ccagttgccc acggtgttca tgtaggcgga ggtggcgccg acgcgcagca
    10621 gccaggtccg ccccgcaaag gtgagggggg tttcccagtt caggcgcttg ccggcccagc
    10681 ccgcctgctc gcaccccgcc gggccgtaga ggcgctcgct ggcgccggtc cggggttcgc
    10741 tcaggcagac gtcgagctcc ccggtccggc ggtgcccgag gatggcctgc atggcgtcgt
    10801 ccatgcggaa caccgccgac accaggccgg tcatgtgcgg cggcgagccg gccagcaccg
    10861 cctgatacac gacgatgccg cgctgctcgc cggtttcctg gacgaggcgg atggattcgc
    10921 tggcgatggg caggccggac ttgcggctgc gctgcacggc gctggcggtc ctgtccagca
    10981 cgaggggatc gagcccgagc acgctgcgat tttcggcgag gggctcgacg aacaggatgg
    11041 gcaggtattc ggccttgcgc gcggccggaa aggtctgccc ggcaacgttg cggccaagga
    11101 tgcggaaatc cggccgcccg gccgcccgca cgcgggcttc gaaggcgtcc cgctgcgcgt
    11161 cggtgacgag gggactccag ccgaagttct gggtgcccgg attgcgggcc atcatcggcg
    11221 tgacgaaatc gcgcca
//
LOCUS       DAOQPA010000110        11227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_2985_length_11227_cov_19.662281, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000110 DAOQPA010000000
VERSION     DAOQPA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_11227_cov_19.662281"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PKX01_13660"
     CDS             complement(<1..866)
                     /locus_tag="PKX01_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HMZ77223.1"
                     /translation="MDITELLAFTVKNKASDLHLSAGLPPMIRVHGDVRRINLPALVQ
                     KDVHGMVYDIMNDTQRKQYEEHLETDFSFEIPNLARFRVNAFNQNRGAAAVFRVIPSK
                     VLTLEELNCPKIFKDICNQPRGIVLVTGPTGSGKSTTLAAMIDYINENEYGHILTVED
                     PIEFVHESKRCLINQREVARDTLSFNAALRSALREDPDKILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKDMVRAMLSESLRAVISQTLLKTKDGSG
                     RVAAHEIMIGTPA"
     gene            997..1680
                     /locus_tag="PKX01_13665"
     CDS             997..1680
                     /locus_tag="PKX01_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ77224.1"
                     /translation="MTGISVGLQKVRDRISTAAQAVGRDPHGVTLLAVSKTWPAECVR
                     AAAAAGQRAFGENYVQEGCAKVDALADPDLEWHFIGPLQSNKTRSVANRFVWVHSIDR
                     LRIAERLSAQRDVHLPPLNVCLQVNVSGEASKSGVAPGEVADLAAAVAALPRLRLRGL
                     MAIPEPTDDVALQRRRFASVRALRDELNAAGFGLDTLSMGMSADLEAAIAEGATLVRV
                     GTAIFGARN"
     gene            1713..2525
                     /gene="proC"
                     /locus_tag="PKX01_13670"
     CDS             1713..2525
                     /gene="proC"
                     /locus_tag="PKX01_13670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMZ77225.1"
                     /translation="MKITFLGGGNMATALIGGLVKQGFDATGVQVVELSAEGREKLAT
                     QFGVRAVDALDDTALACDVLVLAVKPQQLRAAVAPFAGRLGAQVVVSIAAGLRLADIG
                     RWLGGYRTLVRAMPNTPALIGAGVTGLYADASVSAEGRAAAEQVLAAVGSTVWVPDEA
                     QIDAVTAVSGSGPAYVFHFIECLEAAGTALGFDAATARKLALDTVLGAAKLAAGSDEP
                     PSVLRERVTSKGGTTEAALKSFAADGLPAIIARAVAAAEARGRELGEQLGRD"
     gene            2538..3110
                     /locus_tag="PKX01_13675"
     CDS             2538..3110
                     /locus_tag="PKX01_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HMZ77226.1"
                     /translation="MFGSLILTIVDTLGSLLAGVLLARFVMQWQRVSFRNPLGQFVLA
                     TTDWLVIPLRRLVPGLFGLDMASLLPAWVVQTLVVFVTLWVHGVIGRADPLSLLAVMW
                     GVGLLETVKVALYLTMGVVLMSAIFSWVNPHAPLAGVVYALADPFLRPFRRLVPLIGG
                     VDLSPIVLLFVLQIALSALAFLRFSLLGMA"
     gene            3118..3411
                     /locus_tag="PKX01_13680"
     CDS             3118..3411
                     /locus_tag="PKX01_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HMZ77227.1"
                     /translation="MSWFSRTADGGLVLNLHIQPGAKKTECAGLHGEALKIRLAAPPV
                     DGKANAALVAYLAEMLRVPKSAVVLVSGETSRAKRVRVSGAEEGRVAALAARG"
     gene            3561..4001
                     /locus_tag="PKX01_13685"
     CDS             3561..4001
                     /locus_tag="PKX01_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HMZ77228.1"
                     /translation="MVFEWWHWLVLGIALVIAELAVPAFFLIWFGLGGLLVGLALLVL
                     PLGGTAQIALWVAASLAMVFLWFKVFRRGEFKTLIGQSDAHVLGEIGLLSQAVAPFER
                     GRVRFQKPVLGADEWVCRADEAIAAGERVKVVAVEGSFLKVSKV"
     gene            4012..4863
                     /locus_tag="PKX01_13690"
     CDS             4012..4863
                     /locus_tag="PKX01_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ77229.1"
                     /translation="MSNGMVVVLAVLVFALVTLAKGVRIVPQGEEWIVERLGKYFGTL
                     RPGLNLIIPYLDRVAYRMVTKDIILDVQEQEVITRDNAVILTNAIAFLKVTDPVKAVY
                     GVTDFSEAIRNMIMTTLRSIIGEMELDEALSSRDKIKARLRESIADEAVDWGLTVKSV
                     EIQDIKPSESMQRAMEQQASAERERKAVVTRAEGAKQAAILEAEARLESSKRDANAQV
                     MLAEASAEAIRRVTAAVGTETAPMLFLLGEKYIASLERLGASENARVVLLPADLQDAV
                     KGILGKR"
     gene            4927..5904
                     /locus_tag="PKX01_13695"
     CDS             4927..5904
                     /locus_tag="PKX01_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77230.1"
                     /translation="MLAYILRRLLYAPLILIGVNLLTFALFFVVNTPDDMARMQLGVK
                     RVTPEAIERWKVERGYDKPLMYNTAAQGLDALTDTIFFRKSVRMFVFDFGRADDGRDI
                     AQEIRSRMGPSLAVALPTFILGLFVTVSLALLLVFFRGTYLDFTGVVLCVAAMSISGL
                     FYIIGGQWLVSKILHLVPISGYGEGLDASRFLILPVAIGVIAGIGGSARWYRTVFLEE
                     ADRDYVRTARAKGLSELQALFGHVLKNGMIPILTGVVVVIPSLFMGSLITESFFGIPG
                     LGSYTIDAINAQDFPVVRAMVFIGSVLYIIGLILTDLSYTLADPRVRLE"
     gene            5901..7319
                     /locus_tag="PKX01_13700"
     CDS             5901..7319
                     /locus_tag="PKX01_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77231.1"
                     /translation="MRGVQPVLLGTDVLIFALLALVALVIWRARRSEPLRDAWRRVGE
                     SAVGMASLGVLLCFVLVGGLDSLHYRERLAPPAGAAPGEKGPLSTEVRSALDALAAPL
                     RARTEKTYSAPLALHLFARETVERPDGSQSREFPRLRHAGKDLRYPESDHVGDVIGRG
                     GLGLAAGLGVWAGVAAVVVLGVARSRGVPVGAAWRALSRGKGGLAWRALLLTLLGVLA
                     IAGPALMLAAEYHVFGTDKVGQDVLYLTLKSIRTALVIGTLTTLLTLPLAIALGILAG
                     YLGGWVDDLIQYVYTVLNSIPGVLLIAAAVLMMQVVIDTHPHWFDTAADRADARLLAL
                     CFILGMTSWTSLCRLLRGETLKLRELEYVQAARAFGVSTARILLRHVLPNLMHLVLIA
                     VVMDFSGLVLAEAVLSYVGIGVDPNTVSFGTMINAARMELAREPMVWWSLAAAFVFMF
                     VLVLAANLFADVVRDAFDPRSV"
     gene            complement(7329..8747)
                     /locus_tag="PKX01_13705"
     CDS             complement(7329..8747)
                     /locus_tag="PKX01_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HMZ77232.1"
                     /translation="MNTTERLASLDMTWLQLDRETNRLHVTGVLVFDTRLRRDRLEDI
                     LRRRLLRLDRFGQHVIRDTVGAIWESGRVDLDYHVQTFALPDAADPRHALQALVGRLA
                     STPLDPGRPLWQLHLVEKYRHGSAVVVRVHQCIADSIALIGVVQSLTDDHPTTPEDAI
                     PPAGQFDASFFEHLYAPLTQAMIEGIKLSGAAWAKYWSLLFYPQQVFDYARTNAALTM
                     EVGKLMGMRDDSRTCLKGTPQGTKLASWSAPLAASSVQAAARTHRATNDATLLACLAG
                     ALRRFFVRQGDGAEGVELRALVPVNLRGEKARDPLGNRGGILPLALPVGIDDPVTRLA
                     EIQRRLEDFEDAYRAHDTLGLFNLIGQTPRAVQAQALRLVSAKASALLCHVPAASEAR
                     YVDGARISEQMFWSPVTGDMGLSMSILSYAGHYQVGLMVDASMVPDPETITGQLGEEL
                     RLLRNAPASSRKPRSRKAASTS"
     gene            8927..11179
                     /locus_tag="PKX01_13710"
     CDS             8927..11179
                     /locus_tag="PKX01_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77233.1"
                     /translation="MTATIPSPDDVLELLDEDASAAVPGMADAWTVLVVDDDAEVHQA
                     TEFALRDAIVCGRRIALLHATSAAEARARIAGDDDIAVILLDVVMESPDAGLALVHTI
                     RETLGRSEVRIVLRTGQAGYAPEMKVIREYDINDYRTKAELTHTRLLTTLTAAIRAYE
                     QIRRLSVGMRGMREIALSGVQGEIPRSVAEFASVALGRIESLVGRPIQAAFCGPTGDG
                     GTLEDRLGILAGRGSYSCEEGALLSELRDAAWVESIRRARAQQASVFGDRTMCLFLGS
                     RYGKEACVLVDLDRPADEMLRHLVGVLAAHLRVAFENVLLLERLNFFAYFDQLTRLPN
                     RTCFLDRLDAALARLDGGGWVLGIIDIVRFSELNNALGHRSGDALLVDVTRRLGHRLG
                     PEAFLSRLAGDAFGVCWRTGHEEASAVLDVFATPFGVRGHAVPMRARAGFVDVVPVAG
                     GAVELLKRANLALAQAKQQGDQHCSFYASEMEERTQSRVQLLNELRSALHLGQGLELF
                     YQPQVRLGDKMAVGCEALIRWFKPDGEMVSPDVFIPLAEYTGLIVEIGDWVLAEACRQ
                     LVRWDAEGLPPIRVGINISAAQFREPDFVSKVLAQIHETGIDPSRLELEITESVAVDD
                     PESVARQLRELKALGFSVAVDDFGCGFSSLGSLNRLPFDRVKIDRAFVRELTPSTAEG
                     CIARMIVKLAESLGLKVTAEGVETESQAELLESMGCEEMQGFLYGCPMPAPTFQGWMK
                     SRFGTLFAER"
BASE COUNT         1698 a         3313 c         4002 g         2214 t
ORIGIN      
        1 gccggggtgc cgatcatgat ctcgtgggcg gccacgcgcc ccgaaccgtc cttggtcttg
       61 agcagggtct gggagatgac cgcgcgcagg gattcggaca gcatcgcccg caccatgtcc
      121 ttttcggccg cggggaagac gtccacgatc cggtcgatgg tcttggcggc cgacgaggtg
      181 tgcagggtgc cgaacaccag gtggccggtt tcggcggcgg tgagggcgag gcggatggtt
      241 tccaggtcgc gcagttcgcc caccaggatc ttgtccgggt cttcccgcaa tgccgagcgc
      301 agggcggcgt tgaaggacag cgtgtcgcgc gccacttccc gctggttgat gaggcagcgc
      361 ttggattcat gcacgaattc gatcgggtct tccacggtga gaatgtggcc gtactcgttt
      421 tcgttgatgt agtcgatcat cgccgccagc gtggtggact tgcccgagcc ggtggggccg
      481 gtgaccagca cgatgccgcg gggctggttg cagatgtcct tgaagatctt cgggcagttg
      541 agttcttcca gggtgaggac cttggacgga atcacccgga acaccgccgc ggcgccccgg
      601 ttctggttga aggcgttgac ccggaagcgg gcgaggttgg gaatctcgaa ggagaagtcg
      661 gtttccaggt gttcctcgta ctgcttgcgc tgggtgtcgt tcatgatgtc gtacaccatg
      721 ccgtgcacat ccttctgcac cagcgcgggc aggttgatgc ggcgcacgtc gccgtgcacc
      781 cggatcatgg ggggcaaccc ggctgacagg tgcaggtcgg acgctttgtt cttgaccgtg
      841 aaggccagca gttcggtgat atccatggtc ggaaactctc aagaagggcg gtcggtgcga
      901 aggcgcggca aaaagggtcg ccggtgggca gtgctatact cggcggcctt gcaatctacg
      961 gctcgagcct ctccggattt aagcttgaac gattatatga caggcatttc cgtcggcctg
     1021 caaaaggttc gtgaccggat ttcgaccgca gcgcaggcgg tgggacgcga tcctcacggg
     1081 gtgacgctgc tggcggtcag caagacctgg ccggcagagt gcgtgcgggc cgccgccgcc
     1141 gcggggcagc gggcgtttgg cgagaactac gtccaggaag gctgcgccaa agtggacgcg
     1201 ctggcggatc cggacctgga atggcatttc atcggtccgc tccagagcaa caagacgcgg
     1261 tcggtggcca accgcttcgt gtgggtccat tccatcgacc ggctgcgcat cgcagaacgc
     1321 ctgtcggccc agcgcgacgt gcatctgccg cccctcaacg tgtgccttca ggtgaacgtg
     1381 agcggtgaag ccagcaagag cggcgtggcg ccgggcgagg tggccgatct cgccgcggcc
     1441 gtggcggcat tgccgcgcct gcgtctgcgt ggcctgatgg cgattcccga gccaaccgac
     1501 gacgtggccc tgcaacgccg gcgcttcgcc agcgtgcggg cattgcgcga cgaactcaac
     1561 gccgccggat tcggcctcga cacgctctcg atgggcatgt ctgccgatct ggaggcggcc
     1621 atcgccgaag gcgccaccct cgtgcgtgtc ggcacggcca tcttcggtgc ccgcaactga
     1681 gcacatttca tccactccac gaaagcgaac agatgaagat tactttcctg ggcggcggca
     1741 acatggccac agccctgatc ggcggtctgg tcaaacaggg ctttgacgcc accggcgtgc
     1801 aggtcgtcga actctcggcc gagggtcgcg aaaagctggc gacccagttc ggtgtgcggg
     1861 cggtcgacgc gctcgatgac acggcgctgg cctgcgatgt gctggtgctg gccgtcaagc
     1921 cccagcagct gcgggcggcc gtcgcgccct tcgccggccg tctgggagcc caggtggtgg
     1981 tgagcatcgc cgccgggctg cgtctggccg acatcggccg ctggctgggc ggttaccgca
     2041 ccctggtgcg cgccatgccc aacacaccgg cgctgatcgg ggcgggcgtt acggggcttt
     2101 atgccgatgc ctcggtgtct gcggaaggcc gggccgctgc ggagcaggtg ctggcggcgg
     2161 tgggcagcac cgtgtgggtg ccggacgagg cacagatcga cgcggtgacg gcggtgtctg
     2221 gcagcggtcc ggcctacgtc ttccatttca tcgaatgcct ggaggcggcg ggcacggccc
     2281 tgggattcga cgcggccact gcacggaagt tggccctcga tacggtactc ggcgccgcga
     2341 agctggcggc gggtagcgac gaaccgccgt ccgtgctgcg cgagcgggtg acatccaagg
     2401 gcggcaccac cgaggcggcg ctcaagtctt ttgccgccga cggcctgccg gccatcatcg
     2461 cccgtgcggt ggctgccgcc gaggcccgcg ggcgggaact gggcgaacag ctcggccgcg
     2521 actgaggagg acattccatg ttcggcagcc tgatcctgac catcgtggat acgctcggca
     2581 gcctgctcgc gggcgtgctg ctggcgcgct tcgtcatgca gtggcagcgg gtgtccttcc
     2641 gcaatcccct cggccagttc gtgctggcca ccaccgactg gctggtgatt ccgttgcgca
     2701 ggctggtgcc cggtctgttc gggctggaca tggccagcct gctgccggcc tgggttgtgc
     2761 agacgctggt ggtctttgtg accctctggg tgcatggcgt gatcggtcgc gccgatccgc
     2821 tgagcctgct ggcggtgatg tggggcgtgg gcctgctgga gaccgtgaag gtggcgctgt
     2881 acctgacgat gggcgtggtg ctgatgtccg cgatcttctc gtgggtaaat ccccatgcgc
     2941 cgctggcggg cgtggtgtat gcgctggccg accctttcct gcggccgttc cggcgtctcg
     3001 tcccgctcat cggcggtgtg gatctgagcc cgatcgtgct gctcttcgtg ctccagattg
     3061 cgctcagcgc gctggcgttt ctgcgtttct cgctgctggg catggcttga cgcagccgtg
     3121 agctggttca gccgtaccgc cgacggtggc cttgtcctga atcttcacat tcagcctggc
     3181 gcaaagaaga ccgagtgcgc cggtctgcat ggcgaggcgc tgaagatccg tctggccgcg
     3241 ccgccagtgg acggcaaggc caacgcggcc ctggtggcgt atctcgcgga gatgctccgt
     3301 gtgccgaaat cggccgtggt gctggtatcc ggcgagacgt cgcgggccaa gcgggtgcgg
     3361 gtgagcggcg cggaggaggg ccgcgtcgca gctctggcgg cgcggggctg agaaggggta
     3421 ttcgccgtgc gtgggtacag ggtgggggcc atcgctatcc gggccggatc gtccctgctc
     3481 tggtggcact ggcgcccggg gaacgagtcg ttcatgaccg tgtcacagcg acgggttatt
     3541 caggtcctgg gaggcgtccg atggtcttcg agtggtggca ctggctggtc ctgggcatcg
     3601 ccctggtgat cgccgagctg gcggttccgg ccttcttcct gatctggttc gggctgggtg
     3661 ggctgctggt cgggttggcg ctgctggtcc tgcctctggg cggcacggcg cagatcgccc
     3721 tgtgggtcgc cgcttcgctg gccatggtct tcctgtggtt caaggtcttc aggcgcggtg
     3781 agttcaagac cctcattggc cagtcggacg ctcacgtgct gggcgagatc ggcctcctgt
     3841 cccaggcggt ggcgcccttc gagcgggggc gcgtgcgctt ccagaagccg gtgctggggg
     3901 ccgacgaatg ggtgtgtcgc gcggacgagg ccatcgcagc cggtgagcgg gtcaaggtgg
     3961 tggcggtgga aggcagtttc ctgaaggtgt caaaggtgtg aggagcgtgg catgagcaac
     4021 ggcatggtgg tggttctggc agtcctggtc tttgcgctgg tgacgctggc gaagggcgtg
     4081 cgcatcgtgc cccagggcga agagtggatc gtggaacggc tgggtaagta tttcggcacg
     4141 ctccggccgg ggctcaacct gatcattccc taccttgacc gggtggcgta ccggatggtg
     4201 accaaggaca tcatcctcga cgtgcaggag caggaggtca tcacccgcga caatgcggtg
     4261 atcctgacca atgccatcgc cttcctgaag gtgacggatc cggtcaaggc ggtgtatggc
     4321 gtgacggatt tctcagaggc gatccgcaac atgatcatga ccacgctgcg ctccatcatc
     4381 ggcgagatgg aactggacga ggccctgtcg agccgtgaca agatcaaggc gcgcctgcgc
     4441 gagagcattg ccgacgaggc ggtggattgg ggcctgacgg tgaagtcggt ggagatccag
     4501 gacatcaagc cgtccgaatc catgcagcgg gccatggagc agcaggcctc cgccgagcgg
     4561 gaacgcaagg ccgtggtgac gcgggcggaa ggcgccaagc aggcggccat cctcgaagcg
     4621 gaggcgcgcc tggagtcctc caagcgcgat gccaatgccc aggtgatgct ggcggaggcg
     4681 tcggccgagg cgatccgccg ggtgaccgcg gcggtgggga ccgagacggc gccgatgctc
     4741 ttcctgctgg gcgaaaagta catcgcctcg ctggagcgcc tgggggcgtc ggagaatgcc
     4801 cgcgtggtgc tgctgccggc cgatctgcag gacgcggtga agggcatcct cggcaagcgc
     4861 tgacgggatt cgccagacga tgtccgggct tctgttcggc ccgccggccg ggtagcattg
     4921 cgccccatgc tcgcttacat cctgcgccgt ctgttgtacg ccccgctgat cctcatcggg
     4981 gtgaatctgc tgaccttcgc cctgttcttc gtggtgaata cgccggatga catggcgcgc
     5041 atgcaactgg gcgtcaagcg ggtgacgccc gaggccatcg agcgctggaa ggtggagcgg
     5101 ggttacgaca agccgctgat gtacaacacg gcggcgcagg ggctcgatgc cctgaccgac
     5161 acgatcttct tccgcaagtc ggtgcgcatg ttcgtcttcg acttcggccg cgccgatgac
     5221 ggacgtgaca tcgcccagga gatccgcagc cgcatggggc cgtccctggc ggtggcgctg
     5281 ccgaccttca tcctgggcct gttcgtgacg gtgtcgctgg cgctgctgct ggtgttcttc
     5341 cgcggcacct atctggactt taccggcgtg gtgctgtgcg tggcggcgat gtcgatctcg
     5401 ggtttgttct acatcatcgg cggtcagtgg ctggtgtcca agatcttgca tctggtgccc
     5461 atctccggct acggcgaggg gctggacgct tcgcgtttcc tgattctgcc cgtggcgatc
     5521 ggtgtcattg ccggcatcgg cggcagcgcg cgctggtatc gcaccgtctt tctcgaggaa
     5581 gcagaccgtg attacgtgcg caccgcccgg gcaaaggggc tttcggaatt gcaggccctg
     5641 tttggtcatg tactcaagaa cgggatgatc ccgatcctca ccggtgtggt ggtggtgatc
     5701 ccgtccctct tcatgggcag cctgatcacc gaatccttct tcggcattcc cggtctgggc
     5761 agctacacca tcgacgcgat caatgcccag gatttcccgg tggtgcgggc catggtcttc
     5821 atcggatcgg tgctctacat catcggcctg attttgacgg atctgtccta tacgctggcc
     5881 gacccgcggg tgaggctgga atgaggggcg tccagcctgt gctcctgggg accgatgtcc
     5941 tgatcttcgc gttgctcgca ctggtcgcac tcgtcatctg gcgagcccgg cgcagcgagc
     6001 ccttgaggga cgcctggcgc cgggtcggcg aaagcgctgt gggcatggcg tccttgggcg
     6061 tgctgctgtg cttcgtgctg gtgggcgggc tggacagtct ccattaccgg gagcggctcg
     6121 cgccgccggc tggcgcggcg cccggcgaga aggggccctt atccaccgag gtgcgcagtg
     6181 cgctggacgc gttggcggcg cccctgaggg cacggaccga aaaaacctat tctgcgccgc
     6241 tcgccctcca cctctttgcc cgcgagacgg tcgaacggcc cgatggcagc cagagtcggg
     6301 aattcccccg acttcggcat gcgggaaagg atctgcgcta tcccgaatca gaccacgttg
     6361 gcgatgtcat cgggcgaggc gggctggggc tcgccgccgg attgggcgtg tgggcaggcg
     6421 tggctgcggt ggtcgtgctg ggggtcgcgc gcagccgtgg ggtgcctgtc ggcgcggcgt
     6481 ggcgggcgct gtcccgcggc aagggcgggc tcgcctggcg ggcgcttctg ctgaccctcc
     6541 tgggggtgct ggcgattgcc ggaccggcgc tgatgctggc tgcggagtac cacgtcttcg
     6601 ggacggacaa ggtagggcag gacgtgctct atctgacgct caagagcatc cgcacggcgc
     6661 tcgtgatcgg caccctgacg accttgctga cccttcccct ggccatcgcg ctcggcattc
     6721 tggcgggcta tctggggggc tgggtggatg atctgatcca gtacgtctac acggtgctca
     6781 attcgatacc cggtgtgctc ctgattgccg cggccgttct gatgatgcag gtggtgatcg
     6841 acacccatcc gcactggttc gatacggccg cggaccgggc cgacgcgcgc ctgttggcac
     6901 tgtgcttcat tctcggcatg accagttgga ccagcctgtg ccggctcctg cgaggcgaga
     6961 cgctcaagct gcgtgaactg gagtacgtac aagcggcgcg tgcctttggc gtgtctacgg
     7021 cacgcattct gctccgccac gtgctgccta acctcatgca cttggtgctg atcgccgtgg
     7081 tgatggattt ttcaggattg gtgctggcgg aagccgtgct gtcctacgtg ggcatcggcg
     7141 tcgatccgaa cacggtgagt ttcggaacca tgatcaatgc cgcacgcatg gaactggcac
     7201 gggagccgat ggtgtggtgg agcctcgctg cggccttcgt gttcatgttc gtgctggtac
     7261 tggccgccaa cctgtttgcc gacgtggtcc gcgacgcctt cgatccccgc tccgtctgac
     7321 gagaccgctc agcttgtgct ggcggccttg cggctgcgag gtttgcggct gctggcgggc
     7381 gcattgcgga gcaaacggag ttcctccccc agctgtccgg tgatcgtctc gggatcgggc
     7441 accattgatg cgtcaaccat caggccgacc tgataatgcc ctgcgtagct gagaatgctc
     7501 atggacagcc ccatgtcgcc ggtgaccggg ctccagaaca tctgctcgct gatgcgggcg
     7561 ccatccacgt agcgcgcctc ggaagcggcc ggcacgtggc agagcagggc gctggccttg
     7621 gcagagacca gtctgagggc ctgggcctgg acggcgcggg gggtctgacc gatcaggttg
     7681 aagaggccca gggtgtcatg ggcccgatag gcgtcttcga aatcctccag tcggcgctgg
     7741 atctctgcca ggcgggtgac cggatcatcg atgcctacgg gcagggccag cggcaggatg
     7801 ccgccgcggt tgcccagggg gtcccgggcc ttctcgccac gcagattgac ggggacgagg
     7861 gcacgtaatt caaccccctc ggcgccgtca ccctggcgaa cgaaaaagcg cctgagggct
     7921 cccgccagac aggcgagcag ggtggcatcg ttggtggcgc gatgggtacg ggcggcggcc
     7981 tggacgctgg acgctgcgag cggtgcgctc caacttgcga gcttggtgcc ctgaggggtg
     8041 cccttcagac acgtacggga gtcgtcacgc atgcccatca gcttgccgac ttccatggtc
     8101 agcgccgcgt tggtacgggc gtagtcgaag acctgctggg gatagaaaag gagactccag
     8161 tacttggccc atgccgcgcc cgagagcttg atcccttcga tcatggcctg ggtgagaggc
     8221 gcgtagaggt gttcgaaaaa gctggcgtca aactggccgg ccggcggtat ggcgtcttcc
     8281 ggcgtggtgg ggtgatcgtc cgtgagggac tggacgacgc ctataagggc gatgctgtca
     8341 gcgatgcatt gatggacgcg caccaccacg gcgctgccat gccggtattt ttcgaccaga
     8401 tgaagctgcc agaggggacg accggggtcg agcggtgtgc tcgcgagccg gcccacgagc
     8461 gcctgcagtg cgtgacgggg atcggctgcg tcggggaggg cgaaggtctg cacatggtag
     8521 tccaggtcca cccgtcccga ttcccagatg gcgccgaccg tatcgcggat gacatgctga
     8581 ccaaagcgat cgaggcgcag caagcggcgg cgcaggatgt cttcgaggcg gtctcgacgc
     8641 aggcgcgtat cgaacaccag cacgcccgtg acgtggaggc gattggtctc acggtcgagc
     8701 tgaagccagg tcatgtcgag gctggccagg cgttcggtcg tgttcatggc ggtctccggt
     8761 cttgttgtga tcgtgcgatt ggcatggtgt cgccgaaggt tcattgtgcg ccgaatcggt
     8821 gtgttcgtgg gaggaagggc tgcggacgct aaagcccatt catccggggc cgttagtctc
     8881 cgtgaagcgc agtgttgcgc tcgattttcg acccggattc cgtttaatga ccgccacgat
     8941 tccttcgccc gacgacgtac tcgaactcct cgacgaggac gcgagcgccg ctgtgccggg
     9001 aatggcagat gcctggaccg tgctggtggt cgacgacgat gcagaggtgc atcaggcaac
     9061 cgaattcgcg ctgcgtgatg ccatcgtctg cgggcgccgg attgcgttgc tgcatgcgac
     9121 gtcggccgcc gaggcccggg cgcggatcgc tggggatgac gatattgcgg tcatcctcct
     9181 cgatgtggtg atggaaagtc cggatgccgg tcttgccctg gtgcacacca ttcgcgagac
     9241 attggggcgc agcgaggtgc ggatcgtgct tcgcaccggt caagctggct atgcgccgga
     9301 aatgaaggtg atccgcgagt acgacatcaa cgactaccgg acgaaggctg aactgaccca
     9361 cacgcgcttg ctgacgacgc tgacggcagc catcagggcg tatgaacaga tccgccgcct
     9421 gtcggtcgga atgcggggca tgcgggagat tgcgctttcc ggcgtgcaag gggagattcc
     9481 ccgttctgtc gcggagttcg ccagtgttgc gctgggccgc attgagtcgt tggtggggcg
     9541 tccgattcag gcggcgtttt gcggtccgac cggcgacggc gggacacttg aggaccgctt
     9601 gggcatcctt gctggacgcg gcagctattc ctgcgaggag ggggccttgc tgtcggagct
     9661 gagagacgcg gcgtgggtgg agtcgatacg tcgcgcgcgt gcccagcagg catctgtgtt
     9721 cggcgaccgg acgatgtgcc tcttcctggg cagtcgttat ggcaaggaag cctgcgttct
     9781 tgtagatctg gatcgccctg ctgacgagat gttgcggcat ctggtcggcg tcctggccgc
     9841 gcacctgcgg gttgcgttcg agaacgttct cctccttgag cggctgaact tctttgccta
     9901 tttcgatcaa ctcacccgcc tgcccaatcg gacctgtttt cttgatcgtc tcgatgccgc
     9961 gctggcacgc ctcgatggcg gtggctgggt cctgggcatc atcgacatcg ttcgtttctc
    10021 tgaactgaac aacgcgctgg ggcatcgcag tggtgacgcg ctgttggttg acgtgacgcg
    10081 ccgactcggg catcggctcg gtcctgaggc ctttttgtca cgtctggcgg gcgacgcgtt
    10141 tggcgtgtgt tggcgcacgg ggcatgaaga ggcctcggca gtgctcgacg tcttcgcgac
    10201 gccattcggt gtgcgcggac atgcagtccc gatgcgggcc cgcgcgggat tcgtcgacgt
    10261 cgtgcctgtg gctggcggtg cggttgaact cctcaaacgc gccaatctgg cactggctca
    10321 ggccaagcag caaggcgatc agcactgcag cttctatgcc agcgagatgg aggaacggac
    10381 acagagccgg gtacagctgt tgaacgaatt gcggtcggcg cttcatcttg gccagggact
    10441 cgagttgttc taccagccgc aggtgcgcct gggcgacaag atggcggtcg gctgcgaggc
    10501 cctgattcgg tggttcaagc cggatggcga gatggtctcc cccgacgtgt tcatcccact
    10561 ggctgaatac accggactga tcgtcgaaat cggggactgg gtgctcgccg aggcttgccg
    10621 tcagcttgtc cgctgggacg cagaggggct gcccccgatc cgggtgggta tcaacatctc
    10681 ggctgcccag ttccgtgagc cggacttcgt atcgaaggtg cttgctcaga tccacgaaac
    10741 cgggatcgat ccttcgcgcc tggaactgga aatcacggag tccgtcgctg tggatgaccc
    10801 cgaatccgtg gcccggcaac tgagggagct caaagcgctg ggcttcagtg tggccgtcga
    10861 cgacttcggg tgcggttttt cgtcacttgg tagcctcaac cgcttgcctt ttgaccgggt
    10921 gaaaatcgat cgcgcgtttg tgagggagct gacgccctca actgctgaag gttgcatcgc
    10981 ccggatgatc gtcaagctgg ccgaaagctt gggcttgaaa gtgacagctg agggcgtgga
    11041 gaccgagagt caggcggaac tgctggagtc gatggggtgc gaagagatgc agggcttcct
    11101 gtatggatgc ccgatgccgg ccccgacttt tcagggctgg atgaaatccc gcttcggtac
    11161 cttgttcgcg gagcgctgat ttatttttcc aatgttgttg acgtcgttga atagttcttt
    11221 ataatgc
//
LOCUS       DAOQPA010000111        11013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3074_length_11013_cov_17.545903, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000111 DAOQPA010000000
VERSION     DAOQPA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_11013_cov_17.545903"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..782)
                     /locus_tag="PKX01_13715"
     CDS             complement(147..782)
                     /locus_tag="PKX01_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77234.1"
                     /translation="MTVVTLPSGGDFLKACAGHVLAAAPDPAELSRAGLILPNLLLAS
                     DLRAAFHAAAGGAVLLPRLQTLPAVLEPWLAAQPHLPDSRRLFALYHALKARRWLDEA
                     SLWDVCAELVALFDELTLAGVGLPATEAELQMRLAHAYARAVDEPLRFEARLVHVLWQ
                     ADNRGVPSRAAARLLAARAWAAARTAPLFAIAEGALSPFERDVYAACAERV"
     gene            complement(825..1148)
                     /locus_tag="PKX01_13720"
     CDS             complement(825..1148)
                     /locus_tag="PKX01_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HMZ77235.1"
                     /translation="MTYVVTENCIKCKYTDCVDVCPVDCFREGPNFLVIDPEECIDCT
                     LCVAECPAEAIFAEDDVPAGQESYIALNAELALTWTPIVERKDPLPDADDWRGVPDKL
                     QYLER"
     gene            1473..1799
                     /gene="trxA"
                     /locus_tag="PKX01_13725"
     CDS             1473..1799
                     /gene="trxA"
                     /locus_tag="PKX01_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HMZ77236.1"
                     /translation="MSEHIHYVTDANFEAEVLQSQTPVLVDYWAEWCGPCKMIAPILD
                     DVAKEYDGKLKVAKLNIDENQDTPAKFGIRGIPTLMLFKGGNIEATKVGALSKSQLTA
                     FIDSHL"
     gene            1972..3231
                     /gene="rho"
                     /locus_tag="PKX01_13730"
     CDS             1972..3231
                     /gene="rho"
                     /locus_tag="PKX01_13730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMZ77237.1"
                     /translation="MHLSELKSLHVGQLLEMAIANEIEGANRLRKQELVFALLKNRAK
                     KGEPIFGDGVMEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTVEGEIR
                     TPKDGERYFALVKVDKVNFEPPEASKHKILFENLTPLHPNKVLKLERDIRGEENITSR
                     VIDMISPIGKGQRGLLVAPPKSGKTVMLQHIAHSITTNHPDVVVIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPAIRHVQVAEMVIEKAKRLVEHKKDVVILLDSLTRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRVYPAINVNRSGTRREELLLKADVLQKVWVLRKLLYGMDD
                     IEAMEFLLDKIKATKSNGEFFDAMRRG"
     gene            3358..3442
                     /locus_tag="PKX01_13735"
     tRNA            3358..3442
                     /locus_tag="PKX01_13735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3392..3394,aa:Leu,seq:tag)
     gene            complement(3492..4703)
                     /locus_tag="PKX01_13740"
     CDS             complement(3492..4703)
                     /locus_tag="PKX01_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HMZ77238.1"
                     /translation="MKEFAEIADWLGTRGAVHAVPLVESLPAPHFRMDGAEHVSFSTN
                     NYLSLATSPRMIRAAMTGLQRYGVANCESRLLGGDLDVYRTLEDKLARLKGKDTALLF
                     ATGYLTNLGVLAALPRIGQVARTYGYRPRTRHTYAFFSDEYNHVSIREGIRLSGADRF
                     TYRHLDLAHLEHLLNQSAATSRVIVTDGVFSQDGDIAPLPELLALAERYDAMVYVDDA
                     HGSGVLGAHGGGITEHFGIDDPRLICMGTLSKAYGAIGGFIATEQAIGDLLRLTCTAY
                     GFTSTLPPDQAFAVSEALDMVQDEPERLQRLWANQRYFVERMRGLPYRLVATETPIVP
                     VLIGDEALTDHLAACMHEAGLHVDSVKFPAVPRRQGRLRVILNAGHTQEDIDRLVAVF
                     ERHQALCRAAA"
     gene            4901..6070
                     /locus_tag="PKX01_13745"
     CDS             4901..6070
                     /locus_tag="PKX01_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ77239.1"
                     /translation="MLTALRTLPSTVWLLGLVSFFNDTAGELVYPVLPLYLASVLMAG
                     PRALGLIEGIAEATGSLLKLVSGVLSDRLRHTKRWVVAGYAIAGLSRPLLTLATSWPL
                     VLALRFADRIGKGLRSSPRDALLAATVAPQHRGLAFGLHRACDNVGSVAGPLLAAALL
                     ARQVPLERIFLWASLGGAVAVALTLLIREPVRVESRPALRPGWTLATLPPAFRRYLAV
                     LVLFTLGNASNMFLLLKSRDAGVADYQVPLLWALVSFTAALFSTPLSALSDRIGRSRL
                     ILAGWSVYALFYLLLGLAGQAWMMWPLFAFYGLFMAATEGVEKALVADLVPQEQLGTA
                     FGWFNLAAGLTLLPASLIFGALWDALAPTAAFAFSAACAALAALLLRLWVHPERP"
     gene            6154..9552
                     /locus_tag="PKX01_13750"
     CDS             6154..9552
                     /locus_tag="PKX01_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77240.1"
                     /translation="MPDQHAPPVQRVVKIRRDYNTWVARETLEDYALRFTPRNFRRWS
                     ELRVANTAFGAASFLVLEAVGATMLVNAGFINAFWAILATGLIIFLIGLPISHYAARH
                     GLDMDLLTRGAGFGYIGSTLTSLIYASFTFIFFALEAAIMAYALELAFGVPPAWGYLL
                     CALVVIPLVTHGITAISRLQAWTQPLWLVLLILPYLFLLTQEPGVLAELVRYPGANGD
                     GGAFSPQLFGAALTIGIALVTQMGEQVDYLRFMPERTPANRRAWWLGMILGGPGWILP
                     GIAKMLGGALLAYLALRNAVPADKAVDPNQMYLIGFSHVFDHTGLAIAATALFVVVSQ
                     LKINVTNAYAGSLAWSNFFARLTHSHPGRVVWVVFNTVIALLLMELDVFQALGQVLGL
                     YSNIAISWMAAVVADLVINKPLGLAPPGVEFKRSHLYDINPVGVGAMGLASLLSVATF
                     AGAFGATLQPYASFVALGVAFVTAPLIAWATGGRYYLARPAVATGAGLQRCVICERDY
                     EGEDMAHCPAYQGSICSLCCSLDARCHDQCKPHASVAEQWKALLHHLLPHSARAYIDT
                     GLGHYLLLMAGVTALLALVLGVLYTHEALLLGPDQPDLVASLRASFLKLFAALLLLSG
                     VGAWWMVLTQQSRRVAQEESNRQTELLMQEIESHRRTDEQLQRAKEAAEQANQAKSRY
                     ITAISHELRTPLNSILGYAQILAADDAIPPGRRQAVSVIRKSGDHLLSLIEGTLDIAR
                     IEGGKVALEVRPLEFPAFVQQIVAMFELQARNKGLDFTYTPHGALPAVVRADERRLRQ
                     ILINVLGNAVKFTARGGVELAVSYRREMATFDIRDSGPGIPAADLERIFEPFTRGRTG
                     GSPGSGVGLTIARMLTTLMGGELQLSSTPGEGSQFRIRLFLPQLHTAQAEALPRAARV
                     GYVGVRRRILIVDNEKVDRDMLQSVLDPLGFQIEQVGSGEECLVAVPRFAPHLIFMDL
                     AMPGIDGWETIRRLRAGPAPLAGADVKIAILSANAFDKGLDNDAGVAPQDFIVKPLRV
                     DALLDWIGRSLDLEWVHADSPPPPAPAPAAPPPLIAPDASHLAALDELIGLGYLRGIL
                     GKLAEIERLDPCHGEFVRVLREQARQFQFDAMKAILRKARDDRP"
     gene            9539..10471
                     /locus_tag="PKX01_13755"
     CDS             9539..10471
                     /locus_tag="PKX01_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HMZ77241.1"
                     /translation="MTALDRSISDIVLIVDDVPENLALLHDALDDAGHTVLVATDGES
                     ALLRARQSLPDVILLDAVMPGLDGFEVARRLKADFTTRHIPIVFMTGLTETEHVVAAF
                     AAGGADYVTKPIRPAEVLARIAAHVQNARQMKQARSALDAFGQATVAVRVADGRLVWQ
                     TPLARRLLRDFFAAAGEDAPLRLLDWIHDAELARREGREPVGLLAADGSRRLLASFHD
                     QTGDAEWLVVLREENDASAIESLIAAFRLTQKEAEVLYWVTQGKTSKDIGDILGSSPR
                     TVNKHLEHVFEKLGVETRTAAANLAMSRMRSGSA"
     gene            complement(10496..10939)
                     /locus_tag="PKX01_13760"
     CDS             complement(10496..10939)
                     /locus_tag="PKX01_13760"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HMZ77242.1"
                     /translation="MALVSDLPTVAHAIQQSVAPVFLLTGVGSLLGVLANRLGRIVDR
                     SRALRAAGAEAVARAADEMALLQRRSRLVHAAIRYCTLCALLICVVIAVLFVGVELGS
                     DFSSLVAVLFIGGMASLIAGLVCFLREISVATSRLEDRPPPPPPA"
BASE COUNT         1751 a         4276 c         3293 g         1693 t
ORIGIN      
        1 gcttctggtc atacccgcct gaatccgttc gatagttcgc cgtcgtgttg agcgcgtagc
       61 gcatcgccag ccgcaaacgt gacagcagcg cccacatttc tcggtagcaa gccagatcgc
      121 ttgttcgttt cgtaaacagg tggatgttaa acccgctcgg cacaggcggc gtagacgtcc
      181 cgctcgaagg gcgacagggc gccttcggcg atcgcaaaca gcggcgccgt ccgcgccgcc
      241 gcccaggccc gggccgccag cagccgggcg gcggcgcggg acggcacgcc ccggttgtcg
      301 gcctgccaca gcacatgcac gaggcgcgcc tcgaagcgca ggggctcgtc cacggcccgg
      361 gcgtaggcat gcgcaagacg catctgcagc tcggcctcgg tggccggcag accgacgccg
      421 gccagggtca gttcgtcgaa gagggcgacc agctcggcgc agacgtccca caggctggcc
      481 tcgtccagcc agcggcgggc cttgagggcg tggtagagcg caaacagccg gcgggaatcg
      541 ggcagatggg gctgcgccgc gagccagggc tcgagcaccg ccggcagggt ctgcaggcgc
      601 ggcagcagga ccgccccgcc cgccgcggcg tggaaagcgg ctcgcagatc actggcgagc
      661 agcaggttgg gcaggatcag gccggcgcgg gacagctcgg ccggatcagg ggccgccgcc
      721 agcacatggc cggcacaggc cttgaggaaa tcccccccag aggggagggt gacgacggtc
      781 acatcgccat gcaccacggt gccgtgacgg accgccgcgg acgctcagcg ttccagatat
      841 tgcagcttgt ccggcacacc gcgccagtcg tcggcatcgg gcagcggatc cttgcgctcg
      901 acgatgggcg tccaggtcag ggccagctcg gcgttgaggg cgatgtagga ctcctggccg
      961 gcgggcacgt catcctcggc aaagatcgcc tcagccgggc attcggccac gcacagggtg
     1021 cagtcgatgc attcttccgg atcgatcacc agaaagttgg ggccttcacg gaagcagtcc
     1081 accgggcaca cgtcgacgca gtcggtgtat ttgcacttga tgcagttctc ggtaacgacg
     1141 taggtcatga cgttctctct ccttggtggc ccccgccggt gcgccggaaa ggcggcgacc
     1201 gacggcagaa aatcagcgta ttcaggggcc ggaatcatac cccattcggc gttcctgccc
     1261 catccccggc acgcgagcgg cgccgctgtg ccggcgatgc cgccctcgcc gtttcgctcc
     1321 cctcgcccga aaaccgcttt tcttgcctgc caatattcgc taggatcacc tccatccatt
     1381 tcattgcgcg ggcggctccg tcctaagcga gcccggaagc cctcttccct cccggcaatc
     1441 tccttcaaaa atcccctgaa ctgcgaggaa tcatgagcga acatatccat tacgtcaccg
     1501 acgcgaattt cgaagcggag gtgctccagt cccagacccc cgttctggtg gactactggg
     1561 cggagtggtg tggtccctgc aagatgatcg ccccgatcct ggacgacgtg gccaaggaat
     1621 acgacggcaa gctcaaggtc gccaagctca acatcgacga aaaccaggac accccggcca
     1681 agttcggcat ccgcggcatc cccaccctga tgctcttcaa gggcggcaac atcgaagcca
     1741 ccaaggtcgg cgccctgtcc aagtcgcaac tcacggcttt cattgacagc cacctctgag
     1801 ccggttacat tccgtagcgt cagtctcctg cgacgtccgc ggagcggccc cacccggtcc
     1861 cgctccgccg tcgcggtaca gcaagaggca gtcctgcccc tgtacctgca gccgtaacgg
     1921 ctgcccattc ttcccgcctc cttccccttc ttctctcccc ctccgcacgc catgcatctc
     1981 tcggagctca agtctctcca cgtcgggcaa ttgctcgaaa tggccatcgc caatgaaatc
     2041 gaaggcgcca accgcctgcg caagcaggag ctggtcttcg cgctgctcaa gaaccgcgcc
     2101 aagaaaggcg agccgatctt cggcgacggg gtgatggaag tgctgccgga cggattcggc
     2161 ttcctgcgct cgcccgacac ctcctacctg gcgggcaccg acgacatcta cgtgtcgccg
     2221 agccagatcc gccgcttcaa cctgcacacc ggcgacacgg tggaaggcga gatccgcacc
     2281 cccaaggacg gcgagcgcta ctttgcgctg gtcaaggtgg acaaggtcaa cttcgagccg
     2341 cccgaagcct ccaagcacaa gatcctcttc gagaacctca ccccgctgca ccccaacaag
     2401 gtgctcaagc tcgaacggga catccgcggc gaggagaaca tcacctcccg cgtcatcgac
     2461 atgatctccc ccatcggcaa gggccagcgc ggcctgctgg tggcgccgcc gaagagcggc
     2521 aagacggtga tgctgcagca catcgcccac tccatcacca ccaaccatcc cgacgtggtg
     2581 gtgatcgtgc tgctcatcga cgagcgcccc gaggaagtga ccgaaatgca gcgctccgtg
     2641 aagggcgaag tggtcgcctc caccttcgac gagcccgcca tccgccacgt gcaggtggcc
     2701 gaaatggtca tcgagaaggc caagcgcctg gtcgagcaca agaaggatgt ggtgatcctg
     2761 ctcgactccc tgacccgcct cgcccgcgcc tacaacaccg tggtgccggc ctccggcaag
     2821 gtgctcaccg gcggcgtgga cgccaacgcc ctgcagaagc ccaagcgctt cttcggcgcc
     2881 gcccgcaaca tcgaggaagg cggctccctc accatcatcg ccaccgcgct gatcgacacc
     2941 ggcagccgca tggacgacgt gatctacgaa gaattcaagg gcaccggcaa catggagatc
     3001 cacctggatc gccgcatggc cgaaaagcgc gtgtatcccg ccatcaacgt caaccgctcc
     3061 ggcacccgcc gcgaggagct cctcctcaag gccgacgtgc tccagaaagt gtgggtgctg
     3121 cgcaaactcc tctacggcat ggacgacatc gaggcgatgg aattcctcct cgacaagatc
     3181 aaggccacca agagcaacgg cgaattcttc gacgccatgc ggcgcggctg atccgccccc
     3241 ggctcaggcc ccatcgacgc cacctccggg tggcgtcgtg ttttccgggc ggcatcggct
     3301 caggcccgac gaaacactgg tcgcggccct gccccgccag tacaatgcgc ccctcctgcg
     3361 gacgtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag gcgtgggggt
     3421 tcgagtccct tcgtccgcac catgaaaaaa ccggccctct ggccggtttt tgtttgcgcc
     3481 tcgcagacgc ctcaggccgc cgcccggcac aaggcctgat gccgctcgaa caccgccacc
     3541 aggcggtcga tatcttcctg cgtatgaccc gcgttgagga tcacgcgcag gcgcccctgg
     3601 cgtcgcggca ccgccggaaa cttcacgctg tccacgtgca ggccggcctc gtgcatgcac
     3661 gccgccagat gatcggtcag cgcctcgtcg ccgatcagca ccggcacgat gggcgtctcg
     3721 gtcgccacca gccggtaggg cagtccgcgc atgcgctcca cgaaatagcg ctggttggcc
     3781 cacaggcgct gcaggcgctc gggctcgtcc tgcaccatgt ccagcgcctc gctcaccgca
     3841 aaagcctggt ccggcggcag cgtggacgtg aagccgtagg ccgtgcaggt caggcgcagc
     3901 aggtcgccga tggcctgttc ggtggcgatg aagccaccga tggcgccata ggccttgctc
     3961 agggtgccca tgcagatcag acgcgggtcg tcgatcccga aatgttccgt gatgccgccc
     4021 ccgtgggcgc ccagcacgcc gctgccgtgg gcatcgtcca catacaccat cgcgtcgtag
     4081 cgctcggcca gggccagcag ctcgggcagc ggcgcgatgt ccccgtcctg gctgaagacc
     4141 ccgtcggtga cgatcacccg gctggtggcc gcggactggt tgagcagatg ctccaggtgt
     4201 gccagatcca ggtgccggta ggtgaagcgg tcggcgccgg acaggcggat gccctcgcgg
     4261 atgctcacgt ggttgtattc gtcactgaag aaggcatagg tgtgacgcgt gcgcgggcga
     4321 tagccatagg tccgggccac ctggccgatg cgcggcagcg cggcgagcac ccccaggttg
     4381 gtcagatacc ccgtggcaaa aagcagcgcc gtatccttgc ccttgagccg ggccagcttg
     4441 tcctccagag tccgatagac gtccagatcg ccgcccagca ggcgcgattc gcaattggcc
     4501 acgccgtaac gctgcagacc ggtcatcgcc gcgcggatca tgcgcgggct ggtggccagc
     4561 gacaggtaat tgttggtgct gaaggaaacg tgctcggccc cgtccatccg gaaatgtggc
     4621 gccggcaagg attcgacgag ggggaccgca tggacggcac cacgcgtccc gagccagtcg
     4681 gcaatttctg caaattcttt catcgtgcgg ggccgcccat ggacggcagg gataagcgag
     4741 cgcccgatct tagcgacaga cgctgtcagc accttggctc cggtctgacg ccgggctgtc
     4801 acgctgccca tcctgggaag tcccccgccc gggccccctt ccggtatcct gcggcgcgcc
     4861 tgcgcatcat cgcggaggcc cctgccgccg gaccgactcc atgctcaccg ccctgcgcac
     4921 cctgccgtcc accgtctggc tcctcggcct ggtcagcttc ttcaatgaca ccgccggcga
     4981 gctggtctat ccggtgctgc ccctctatct cgcctcggtg ctgatggccg gccccagggc
     5041 cctcgggctc atcgaaggca tcgccgaggc caccggcagc ctgctcaagc tggtctccgg
     5101 cgtgctgtcg gaccgcctgc gccacaccaa gcgctgggtg gtggccggct atgccatcgc
     5161 cggcctgtcg cgccccctgc tgaccctcgc caccagctgg ccgctggtgc tcgccctgcg
     5221 ctttgccgac cgcatcggca agggcctgcg cagctcaccg cgggacgccc tcctcgccgc
     5281 caccgtggcg ccgcaacacc gcggtctggc cttcggcctc caccgcgcct gcgacaacgt
     5341 gggctcggtg gccggtccgc tcctcgccgc cgccctcctc gcccgccagg tgcccctgga
     5401 acggatcttc ctgtgggcca gcctgggcgg cgcggtggcc gtggccctca ccctgctgat
     5461 ccgcgaaccg gtccgtgtcg aatcccgccc ggcgctgcgc cccggctgga ccctcgccac
     5521 cctgccgccg gcgttccggc gctatctggc ggtgctggtg ctgttcaccc tgggcaacgc
     5581 ctccaacatg ttcctgctcc tcaagagccg cgacgccggc gtggccgact accaggtgcc
     5641 gctgctgtgg gcgctggtct ccttcaccgc cgcgctgttc tccacgcccc tctcggccct
     5701 ctccgaccgc atcgggcgca gccgcctgat cctcgccggg tggagcgtct atgccctgtt
     5761 ctatctgctc ctcggcctcg ccggtcaggc ctggatgatg tggcccctgt tcgccttcta
     5821 cggcctcttc atggccgcca ccgaaggggt cgagaaggcc ctggtggccg acctggtgcc
     5881 gcaggaacag ctcggcaccg ccttcggctg gttcaacctg gcggccgggc tgaccctgct
     5941 gccggcttcg ctgatcttcg gcgccctgtg ggacgccctc gcccccaccg cggccttcgc
     6001 cttctcggcc gcctgtgccg cgctggccgc gttgctgctg cgcctgtggg tgcacccgga
     6061 acggccctga ggcgtccccg ccgccgcccc accccctgcg cagtcctgcg cattgtgggc
     6121 gtccgcccac ctgcggtata agaaccgcca cccatgcccg accagcacgc ccctcccgtc
     6181 cagcgcgtcg tcaagatccg gcgcgactac aacacctggg tcgcccgcga gaccctggag
     6241 gactacgccc tgcgcttcac gccgcgcaac ttccggcgct ggtccgagct gcgcgtcgcc
     6301 aacaccgcct tcggcgccgc ctcatttctg gtgctggaag ccgtgggcgc cacgatgctg
     6361 gtgaacgccg gcttcatcaa cgccttctgg gccatcctcg ccaccggcct catcatcttc
     6421 ctgatcggcc tgcccatcag ccattacgcc gcccgccacg ggctggacat ggacctgctc
     6481 acccgtggcg ccggcttcgg ttacatcggc tcgacgctca cctccctcat ctacgccagc
     6541 ttcaccttca tcttcttcgc gctggaggcc gccatcatgg cctacgccct ggagctggcc
     6601 ttcggcgtcc cccccgcctg gggctacctg ctctgcgccc tggtggtgat tcccctggtc
     6661 acccacggca tcaccgccat cagccgcctg caggcgtgga cccagcccct gtggctggtc
     6721 ctgttgatcc tgccttacct cttcctgctc acccaggagc ccggcgtgct cgccgagctt
     6781 gtccgctatc ccggcgcgaa cggcgatggc ggcgccttca gcccccagct cttcggcgcc
     6841 gccctcacca tcggcatcgc cctggtcacc cagatgggcg agcaggtgga ctacctgcgc
     6901 ttcatgcccg aacgcacccc cgccaaccgc cgcgcctggt ggctgggcat gatcctcggc
     6961 gggcccggct ggatcctgcc cggcatcgcc aagatgctcg gcggcgccct cctcgcctac
     7021 ctggccctgc gcaacgcggt gcccgccgac aaggccgtgg atcccaacca gatgtacctg
     7081 atcggcttct cccacgtctt cgaccacacc ggcctggcca tcgccgccac cgccctcttc
     7141 gtcgtcgtct cccagctcaa gatcaacgtc accaacgcct acgccggctc cctcgcctgg
     7201 tccaacttct tcgcccgcct cacccacagc caccccggcc gggtcgtgtg ggtggtgttc
     7261 aacaccgtca tcgccctgct gctcatggag ctggacgtct tccaggccct cggccaggtc
     7321 ctcggcctct actccaacat tgccatctcg tggatggctg cggtggtggc cgacctggtc
     7381 atcaacaagc ccctgggcct cgccccgccg ggcgtcgagt tcaagcgcag ccacctctac
     7441 gacatcaacc ccgtgggtgt cggggcgatg gggctggcct ccctgctgtc ggtggccacc
     7501 ttcgccggcg ccttcggcgc gaccctgcaa ccctatgcct ccttcgtcgc cctgggcgtc
     7561 gccttcgtca ccgcgcccct catcgcctgg gccaccggcg ggcgctacta cctcgcccgc
     7621 ccggcggttg ccaccggtgc cggcctgcaa cgctgcgtca tctgcgagcg ggactacgaa
     7681 ggcgaggaca tggcccactg ccccgcctac cagggttcca tctgctccct gtgctgctcg
     7741 ctggatgccc gctgccacga ccagtgcaag ccccacgcca gcgtcgccga gcaatggaag
     7801 gccctgctcc atcacctcct gccccactcc gcccgcgcct acatcgacac cggcctgggg
     7861 cactacctgc tcctcatggc cggcgtcacc gccctgctgg ccctcgtgct gggggtgctc
     7921 tatacccacg aagccctgct cctcggcccg gaccaacccg acctcgtcgc ctccctgcgg
     7981 gccagcttcc tcaagctctt cgccgcgctg ctgctgctct ccggcgtggg cgcctggtgg
     8041 atggtgctca cccagcagag ccgccgggtc gcccaggagg aatccaaccg ccagaccgaa
     8101 ctcctcatgc aggagatcga atcccaccgc cgcaccgacg aacagctcca gcgcgccaag
     8161 gaagccgccg agcaggccaa ccaggccaag agccgctaca tcaccgccat cagccacgag
     8221 ctgcgcacgc ccctcaacag catcctcggc tatgcccaga tcctcgccgc cgacgacgcc
     8281 atccccccgg gccggcgcca ggccgtcagc gtcatccgca agagcggcga ccacctcctg
     8341 tccctcatcg aaggcaccct ggacatcgcc cgcatcgaag gcggcaaggt cgcgctggaa
     8401 gtgcgcccgc tggaattccc cgccttcgtc cagcagatcg tcgccatgtt cgagctgcag
     8461 gcccgcaaca agggcctgga cttcacctac accccccacg gcgcgctgcc cgccgtggtc
     8521 cgcgccgacg aacgccgcct gcgccagatc ctcatcaacg tgctcggcaa cgccgtcaaa
     8581 ttcaccgccc gcggtggcgt cgagctggcc gtgagctacc gccgcgagat ggccaccttc
     8641 gacatccgcg acagcggccc cggcatcccc gccgccgacc tggagcgcat cttcgaaccc
     8701 ttcacccgcg gccgcaccgg cggctcgccg ggcagcggcg tcggcctcac catcgcccgc
     8761 atgctcacca ccctcatggg cggcgagctg cagctcagca gcactccggg cgaaggcagc
     8821 cagttccgca tccgcctgtt cctgccccag ctccacactg cccaggccga ggccctgccc
     8881 cgcgccgccc gggtcggcta tgtgggcgtg cgccgacgca tcctcatcgt ggacaacgaa
     8941 aaggtggacc gggacatgct gcagagcgtc ctcgaccccc tcggcttcca gatcgagcag
     9001 gtgggcagcg gcgaagaatg cctcgtcgcc gtgccccgct tcgcccccca cctgatcttc
     9061 atggacctcg ccatgcccgg catcgacggc tgggagacca tccgccgcct gcgcgccggc
     9121 cccgcgcccc tggccggcgc cgacgtaaag atcgccatcc tctccgccaa cgccttcgac
     9181 aagggcctcg acaacgacgc cggcgtggcg ccgcaagact tcatcgtcaa acccctgcgg
     9241 gtggacgccc tgctcgactg gatcggccgc agcctcgacc tggagtgggt ccacgccgac
     9301 agcccgccgc cccccgcgcc ggccccggcc gccccgccgc ccctcatcgc gccggacgca
     9361 tcccacctcg ccgccctgga cgaactcatc ggcctgggct acctgcgcgg tatcctcggc
     9421 aagctcgccg agatcgaacg cctcgacccc tgccacggcg aattcgtccg cgtcctgcgc
     9481 gaacaggccc gccagttcca gttcgacgcc atgaaagcca tcctccgcaa ggcccgcgat
     9541 gaccgccctt gaccgcagca tctccgacat cgtcctcatc gtggacgacg tgcccgagaa
     9601 cctcgccctg ctccacgacg ccctcgacga cgccggccac accgtgctcg tcgccaccga
     9661 cggcgaatcc gccctgctgc gcgcccgcca gagcctgccc gacgtgatcc tgctggatgc
     9721 cgtgatgccc ggcctggacg gctttgaagt ggcccgccgg ctcaaggccg acttcaccac
     9781 ccgccacatc cccatcgtct tcatgaccgg cctcaccgag accgaacacg tggtcgccgc
     9841 cttcgccgcc ggtggcgccg actacgtcac caagcccatc cgccccgccg aagtcctcgc
     9901 ccgcatcgcc gcccacgtcc agaacgcccg ccagatgaag caggcccgca gcgccctgga
     9961 cgccttcggc caggccaccg tcgccgtgcg cgtggccgac ggccgcctgg tctggcagac
    10021 ccccctcgcc cgccgcctgc tgcgcgactt ctttgccgcc gccggcgagg acgccccgct
    10081 gcgtctcctc gactggatcc acgacgccga gctcgcccgc cgcgaaggcc gcgagcccgt
    10141 cggcctgctc gccgccgacg gcagccgccg cctcctcgcc tccttccacg accagaccgg
    10201 cgacgccgaa tggctcgtcg tgctgcgcga agagaacgac gcctccgcca tcgaatccct
    10261 catcgccgcc ttccgcctca cccagaaaga ggccgaagtc ctctactggg tcacccaggg
    10321 caagaccagc aaggacatcg gcgacatcct gggcagcagc ccccgcaccg tgaacaagca
    10381 cctggaacac gtcttcgaga agctcggcgt ggagacccgc accgccgccg ccaacctggc
    10441 catgagccgg atgcgcagcg gcagcgcctg aacgcccgcc gcgacacccg gcgcgtcacg
    10501 ccggcggggg tggcggaggc cgatcctcca ggcgcgaggt ggcgaccgag atctcccgca
    10561 ggaaacacac cagcccggcg atcagcgacg ccatcccgcc gatgaagagc accgccacca
    10621 gcgacgagaa atcgctgccc agctccaccc ccacgaacaa caccgcgatc accacgcaga
    10681 tcagcaaggc acacagggtg caatagcgga tcgctgcatg gaccaggcgc gaacgccgct
    10741 gcagcagcgc catctcgtcc gcggcgcggg ccaccgcctc ggcgccggcc gccctcagcg
    10801 cgcgcgaacg atccacgatg cgaccgagcc gattggccag caccccgagc agggagccca
    10861 ccccggtcaa caggaacacc ggcgccacgg actgctggat cgcgtgggcc accgtaggca
    10921 gatcggacac cagggccatg actcagcgcc cccccaatcc gcgacgcaag gcgccgccgc
    10981 ccgcccacgc cgcccgcccc gccccctggc cca
//
LOCUS       DAOQPA010000112        10854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3140_length_10854_cov_19.417446, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000112 DAOQPA010000000
VERSION     DAOQPA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10854
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_10854_cov_19.417446"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="def"
                     /locus_tag="PKX01_13765"
     CDS             complement(<1..478)
                     /gene="def"
                     /locus_tag="PKX01_13765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ77243.1"
                     /translation="MALLPILRYPDPRLHTRAVPVREVTDEIRTLVANMAETMYEAPG
                     IGLAATQVDVHKRIVVIDVSEDKTGLLALINPEIIERSGEQVCEEGCLSVPGIYDKVS
                     RAEKVRVRALNLQGETFEFDAEGLLAVCVQHELDHLEGKVFVEYLSLLKQTRIKSKL"
     gene            659..1771
                     /locus_tag="PKX01_13770"
     CDS             659..1771
                     /locus_tag="PKX01_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HMZ77244.1"
                     /translation="MRRIISALLVGVAALVPAGAGATDPVRLAEDAPDSHTVVPGDTL
                     WGISGTFLKEPWRWPEVWQLNRDQIRNPHWIYPGQVVVLDRSGANPVLRLGKRIGASP
                     ADRPLYEKRFPQVYSESARDAIPSIPIRVIQPFLVEPLVVEDKDDERAGIVVATQEGR
                     VFTGPGDTIFATRVPPGVPLWNVYRKAQPLKEPVTGEILGYEAAFLGTARVTAEMGVL
                     DADAGVKGEADQPPVPATLQILTVKQEVGKGDRLLPAPKPELLSYVPHAPDADVAGRV
                     VSVYNGVTETGRFNVIAVSLGKRDGMEIGHVLSLHRNRGEAVYREDNVGPAQRYKLPE
                     QRYGLVFIFRVFDRIAYGLVMQSGGVVSVADSVRKP"
     gene            1880..2968
                     /gene="dprA"
                     /locus_tag="PKX01_13775"
     CDS             1880..2968
                     /gene="dprA"
                     /locus_tag="PKX01_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMZ77245.1"
                     /translation="MAESHPLAPWLRLTLVPGVGAERQRQLLAAFGPPDAVFAAGHAA
                     LAAIIGSPAARQLLDHDTADAVARSLDWLAQSGNRILTLGDPDFPPALLEIPDPPVLL
                     YIKGDVTLLSRRGVAVVGARSATPQGEANAEAFATALSAAGLPIVSGLALGIDSAAHR
                     GGLGGPGRTVAVIGTGADRIYPARNEALARRIAEAGAIVSELPLGAPPLAHHFPRRNR
                     LIAGLARGVLVVEAAVQSGSLITARLAAECGREVFAIPGSIHSPLARGCHRLIRDGAK
                     LAESAEDVLEELQLEGTMPPAAAASPPDEGDPQAAQVVSALGHDPVDMDTLLQRTSLT
                     SAELYAILLTLELDGRVSRLPGGRVQRL"
     gene            2996..3472
                     /locus_tag="PKX01_13780"
     CDS             2996..3472
                     /locus_tag="PKX01_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HMZ77246.1"
                     /translation="MFDILVYLFENYVHADACPEPAQLARKLSAAGFEEEEITEALEW
                     LSGLRELSDGDRPAHVARAGSVRIYAPEEQVLLGIECRGFLQFMENAGVLDAECREMI
                     VERALALGDPEIELDNLKVIVLMVLWQQDRPINGLILDELLNESDDEDADAPNVLH"
     gene            3645..6194
                     /locus_tag="PKX01_13785"
     CDS             3645..6194
                     /locus_tag="PKX01_13785"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HMZ77247.1"
                     /translation="MSKHLIIAEKPSVAADIARAIGGFTKEKDYFESEHYVLSSAVGH
                     LLELSVPAEFEVKRGKWSFAHLPVIPPHFALNPIEKTEDRLRLLTRLIKRKDVTGLIN
                     ACDAGREGELIFSFIAQHAGTHKPVQRLWLQSMTPGAIRDGFAHLRSADDVAGLREAA
                     VCRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVEREEKIRAFRPTD
                     YWELEARFGCQAGSYAGRWFDEGFRKPEEGTPLAEHASAYRLWDKTRAEALRAKCQGQ
                     PGSVEEESKPTTQLSPLLFDLTSLQREANGRFGFSARTTLQLAQALYEKHKVLTYPRT
                     DARALPEDYVGQVKGILGGLPDAYAPFANEIVKHGWAKPNKRIFNNAKISDHFAIIPT
                     GTPPKTLSEAEAKIYDLVTRRFLAVFYPAAEYRVTTRITRVAGEAFKTEGKVLVQPGW
                     LAVYGKSAALADEDGGTPQLVAVTPGEQVSTDEVALKALVTKPPARFNEATLLSAMEG
                     AGKMVDDEELRAAMAGRGLGTPATRAQIIENLIGETYILRDGKELVPTAKAFSLITLL
                     RGLGTHELTSPELTGEWEHKLAQMERGALSRSTFMEHIQTMARDMVERAKRYESDTVP
                     GDFATLQTPCPKCGGVVKENYKKFACQGCDWSAWKIVAGRQFEIPEMETLLSTGRVGP
                     LMGFRNKMGRLFNAEIRLNDDQQPEFDFGQPKEDEAAEAVDFSAQEALGTCPKCGGGV
                     YEHGLAYVCEKSVGPGKSCDFRSGKIILQQPIEREQMTKLLADGRTELLKGFVSTRTK
                     RKFSAFLVRGADGKVGFEFEKKEPRAKAGDAADGAAPAAKKAAPRRKPAAG"
     gene            complement(6326..7192)
                     /locus_tag="PKX01_13790"
     CDS             complement(6326..7192)
                     /locus_tag="PKX01_13790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ77248.1"
                     /translation="MSSPAVQARPALTHDDLDEALQSIQIPACPAVVAEVMREAQKDD
                     PDMRVLARVIASDVGMSAMAIKLANSALFRATSPVKSVSQAVNRLGISNVLSVVIAAA
                     LRSASASLPSELMDPFWNRAATLALSTAVIARCHMGVSAENAYTFALFHDAAIPVMMT
                     HYPQYGAVYAAAQASGRPLVADELAHFPCSHPTVGWLLARNWGLPPVLAAAIRFHHEP
                     DIFSKPDDSLTPEALGMMAVTLVAEHVLAECIGDTGGELDTGLAQQAMAYLGTSDQDL
                     DDFRERVAAAIR"
     gene            7554..9512
                     /locus_tag="PKX01_13795"
     CDS             7554..9512
                     /locus_tag="PKX01_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HMZ77249.1"
                     /translation="MTASPSSPAALVSLKHCRDRFVDAIRAAARTARCPEPWLDGLCS
                     AAAEGFEEMAGLRERRGFEQLRGLTASRISLIHQEDLDYSIELINLEQRIHDHVHREM
                     TLADRRLHELLIELNLTRGEDSPFGSEVACLALRALTERERINPVEALDFARQIEAPL
                     KIQLIELYRDVDHTLAGVPSYGQKSGRAPAPRPGTPDGQVLSIGDGGHDPVDGLRWAV
                     ISRAGDGLSETDGQDRAALKAVVDRIEAWMDHERQNGEWAHDELSTSELVQLLPPPRV
                     AALAVVEAVFAHALTRADLPGPIRALLVELRFPILKQALRHTDLFEGSSRPPALRLVD
                     SIAQLGASLPPEGTADHSLYRAAAKVVNALVQPDTLPDDAFAVALEALDKRVAERRQT
                     TLERARAFEEKGVHLERREIALHQATRMVSQLLTQAPEGPVRQFLEGYWVQVLTRAAY
                     LRGTDHAEWDSRVRTAAQLIASIVPPADDAERERLRAGLPALRGGLGDGLQAVGLKPE
                     TIDAALAPLDALHAAVLDGRLLPAIPPKPRTPRVHHSPVSGFPGLFALAHKSYLAGEP
                     VLAAEWADIRTGDALVLALPDGGTLRGLVAWIGPGGQLMLIADTDRHDVLAVTFRAFA
                     QQTAQPGSRRLRPFSLVEDAADRRLAHA"
     gene            9600..>10854
                     /locus_tag="PKX01_13800"
     CDS             9600..>10854
                     /locus_tag="PKX01_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HMZ77250.1"
                     /translation="MCSDPACTAAPPAGSDPDAAIRIRGANQNNLKNLSLDIPLDELV
                     VVTGVSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPRVDRIDGVPPAIAI
                     DQTNPVRTSRSTVGTMTELADHFKLLYARGARLHCRGCGRPVRRDTPASIHATLAERA
                     AAAGDPRLVVSFPVSVPAAFSEAEIAELLARQGYTRVQARDGDVRHVVQDRFRLGNAE
                     HARVMEALETALRAGHGRLTVHALGDAGEARWPFSAGLHCAECDIAYADPVPSLFSFN
                     SPLGACDTCRGFGRVIGVDFGLVIPDETRTLAEGAVKPWQSPSFKECHDDLVKMAGKY
                     GVPLDVPVADLSPEHRQWLLEGDARWKSWDSSWPRYWYGVRHFFDWLESKAYKMHIRV
                     LLSRYRSYTECPACHGARLKPDALLW"
BASE COUNT         1754 a         4021 c         3479 g         1600 t
ORIGIN      
        1 ccagtttgct cttgatgcgg gtctgcttga gcagggacag gtattccacg aagaccttgc
       61 cttccaggtg gtcgagttcg tgctgtacgc acacggcgag gaggccctcg gcgtcgaatt
      121 cgaaggtctc gccctgcaga ttgagggcgc gcacgcggac cttctcggcg cggctcacct
      181 tgtcgtagat gccgggcacc gacagacacc cttcctcaca gacctgctcg ccggaacgtt
      241 cgatgatctc cgggttgatg agggccagga ggccggtctt gtcctccgac acgtcgatga
      301 cgacgatgcg cttgtggacg tccacctggg tggcggccag gccgatgccg ggggcttcat
      361 acatggtttc ggccatgttg gcgacgaggg tgcggatctc gtcggtcacc tcgcgcaccg
      421 gcacggcgcg ggtatggaga cgggggtccg gataacggag gatgggtaga agggccataa
      481 gattgccgaa tgaagacttt acgtgcagaa ttttgagcga aatctgcgat gcccgcgtcc
      541 ttgtgccggg ccttgcccat caggacaccg tccaccctgc gcagcacggg tgggcgcaca
      601 ccccagaccg cccggatcgc ccggcggtca acggccgaaa gcgagtgcac ttccgatgat
      661 ccgccgcatt atatctgccc tcctcgtcgg cgtcgccgcc ctggttcccg cgggagccgg
      721 cgcgacggat ccggtccgcc tggccgagga cgcgcccgac agtcacaccg tggtgcccgg
      781 cgacaccctg tggggcatat ccggcacctt cctcaaggag ccctggcgct ggcccgaagt
      841 gtggcagctc aaccgggacc agattcgcaa cccccactgg atctacccgg ggcaggtcgt
      901 cgtgctcgac cgcagcggcg ccaaccccgt gctgcggctg ggcaagcgca tcggcgccag
      961 cccggccgac cggccgctct acgagaagcg cttcccgcag gtgtattccg agtcggcgcg
     1021 ggacgcaatc ccgagcattc cgatccgcgt catccagccc ttcctggtgg agccgctggt
     1081 cgtcgaggac aaggatgacg aacgcgccgg catcgtggtc gccacccagg aaggtcgtgt
     1141 gttcaccggt cccggcgaca ccatcttcgc gacgcgcgtc ccgccggggg tgccgctctg
     1201 gaatgtctac cgcaaggccc agcccctcaa ggagccggtc accggcgaga tcctcggcta
     1261 cgaggccgcc ttcctcggca cggcccgcgt gaccgccgag atgggcgtgc tcgatgccga
     1321 cgccggcgtc aagggcgagg ccgaccagcc gccagtcccc gccacactgc agatcctcac
     1381 ggtcaagcag gaggtcggca agggcgaccg cctgctcccg gcccccaagc cggaactgct
     1441 cagctacgtg ccccacgccc cggacgccga cgtggcgggc cgcgttgtct cggtctacaa
     1501 cggcgtcacc gagaccggcc gcttcaacgt gatcgccgtg agcctgggca agcgcgacgg
     1561 catggaaatt ggccacgtcc tgtccctgca ccgcaatcgc ggcgaagcgg tctatcgtga
     1621 agacaacgtc ggcccggccc agcgctacaa gctgccggag cagcgttacg gcctggtctt
     1681 catcttccgc gtcttcgacc ggatcgctta cggtctggtg atgcagagcg gcggcgtggt
     1741 cagtgtcgcg gacagcgtgc gcaagccctg acggccccgc gccgcggcaa atccgacgca
     1801 gcggcgcccg ccaaggcacc ggtccgcgcc gtcaccgttc gtcctcccac cgcgcagccc
     1861 tgctcccgtc cgtttccgcg tggccgagtc ccatcccctc gctccctggc tgcgtctgac
     1921 gctggtgccc ggtgttggcg ccgaacgcca gcgtcagctt cttgccgcct ttggtccccc
     1981 ggacgccgtc ttcgcggcgg gtcacgcggc cctcgctgcc atcattggca gccctgcggc
     2041 ccgccaactg ctcgatcacg atactgccga cgcagtcgcc cgcagcctcg actggctcgc
     2101 ccagtcgggc aatcgcatcc tcaccctcgg cgatccggac ttcccgccgg cgctgctgga
     2161 aattcccgac ccgccggtcc tcctctatat aaaaggtgac gtcaccctgt tgtccaggcg
     2221 cggcgtcgcc gtggtcggcg cgcgcagcgc cacccctcag ggcgaagcca atgccgaagc
     2281 cttcgccacc gccctgtcgg ccgccggtct gcccatcgtc agcggcctcg ccctgggcat
     2341 cgacagcgcc gcccaccggg gcgggctggg cggtccgggg cgcaccgtgg ccgtgatcgg
     2401 gaccggcgcc gaccggatct atccggcgcg caatgaagcc ctcgcccgcc ggatcgccga
     2461 ggccggcgcc atcgtcagtg aactgcccct cggggcgccg ccgctggccc accattttcc
     2521 gcgtcgcaac aggctgatcg ccggcctggc ccgcggcgtg ctggtcgtgg aagccgccgt
     2581 gcagagcggg tcgctgatca ccgcccgcct ggccgccgag tgcggtcgcg aggtcttcgc
     2641 gatccccggc tccatccact cgcccctcgc ccgcggctgc catcgcctga tccgcgacgg
     2701 cgccaagctg gccgaatccg cggaagacgt gctcgaggag ctgcagctcg aaggcacgat
     2761 gccgcctgcc gccgccgcgt cgccccccga cgagggtgac ccgcaggccg cgcaggtcgt
     2821 gtccgcactc ggccacgacc ctgtggatat ggacacgttg ctgcaacgca ccagcttgac
     2881 gtcagcggaa ctctacgcca ttctgctcac gctggaactc gatgggcggg tttcccgcct
     2941 tcccggaggc cgcgtacagc gcctctaatt cgaccggcct caggccccgc gcgccatgtt
     3001 cgacatcctc gtatatctgt tcgagaacta cgtccacgcc gacgcctgcc ccgaacccgc
     3061 ccagctcgcg cgcaagctct ctgccgccgg cttcgaggaa gaggagatca ccgaggccct
     3121 ggagtggctg tcgggcctgc gcgaactctc cgacggcgac cgtcctgccc acgtggcccg
     3181 cgccggctcg gtacgcatct acgcgcccga ggagcaggtc ctgctgggca tcgaatgccg
     3241 cggattcctc cagttcatgg aaaacgccgg cgtcctcgac gccgagtgcc gcgagatgat
     3301 cgtcgagcgt gccctcgctt tgggtgatcc cgagatcgag ctggacaacc tcaaggtcat
     3361 cgtgctgatg gtgctgtggc agcaggaccg tcccatcaac ggcctgatcc tcgacgagct
     3421 cctcaacgag tccgacgacg aggatgcgga cgcccccaac gtcctgcact gagccacact
     3481 ccgcttgcaa gctgcgcgcc ggcttccgta tgatgcggcg cacccccacc ccaaccatct
     3541 tgaatagtgc gggttgagcg cttgtgcgcc gggctgaccg cccgaacgcg caaggggttc
     3601 gttcccgccc ttgagaggca agcgtcgaag agcgccagac cggcatgagc aagcacctca
     3661 tcatcgcgga aaaaccctcc gtcgcggccg acatcgcccg cgccatcggc ggtttcacca
     3721 aggaaaagga ctacttcgag tcggagcact acgtgctctc gtcggccgtt ggccacctgc
     3781 tcgaactctc cgtcccggcc gaattcgagg tcaagcgggg caagtggtcc ttcgcccacc
     3841 tgccggtgat tccgccccac ttcgccctca accccatcga gaagaccgag gaccgcctca
     3901 ggctcctcac ccgcctcatc aagcgcaagg acgtcaccgg cctcatcaac gcctgtgacg
     3961 cgggccgcga gggggaactg atcttcagct tcatcgccca gcacgccggc acccacaagc
     4021 cggtgcagcg cctgtggctg cagtccatga cgcccggtgc gatccgcgac ggctttgccc
     4081 atctgcgctc ggccgacgac gtggcgggcc tgcgcgaggc cgccgtgtgc cgcgccgaga
     4141 gcgactggct gatcggcatc aacggcacgc gggcgatgac cgccttcaac tccaagaccg
     4201 gcggcttcca cctcaccacc gtcggccgcg tgcagacacc caccctggcc atcgtcgtgg
     4261 agcgggagga gaagatccgc gccttccggc ccaccgacta ctgggagctg gaagcccgct
     4321 tcggctgcca ggccggcagc tacgccgggc gctggttcga cgagggcttc cgcaagccgg
     4381 aggagggcac gcccctggcc gagcacgcgt ccgcctaccg cctgtgggac aagacccgcg
     4441 cagaagccct ccgcgccaag tgccaaggcc agcccggcag cgtggaggag gaatccaagc
     4501 ccaccaccca gctctcgccg ctgctcttcg acctcacctc cctgcagcgg gaggccaacg
     4561 gccgcttcgg cttctcggcc cgcaccaccc tgcagctggc ccaggccctt tacgaaaagc
     4621 acaaggtgct gacctacccg cggaccgacg cccgcgccct gcccgaagac tacgtcggcc
     4681 aggtgaaggg catcctcggc ggcctgccgg acgcctacgc gcccttcgcc aacgagatcg
     4741 tcaagcacgg ctgggccaag cccaacaagc ggatcttcaa caacgcgaag atctccgatc
     4801 acttcgccat catccccacc ggcacaccgc ccaagaccct gtcggaagcc gaggccaaga
     4861 tctacgatct ggtcacccgg cgcttcctgg ccgtgttcta cccggccgcc gagtaccgcg
     4921 tcaccacccg catcacccgt gtggccggcg aagccttcaa gaccgagggc aaggtgctgg
     4981 tccagcccgg ctggctggcg gtgtatggga agtcggcggc cctcgccgac gaggacggcg
     5041 gcacgcccca gctggtggcc gtcacgccgg gcgagcaggt cagcactgac gaggtcgccc
     5101 tcaaggctct ggtcaccaag ccgcccgcgc gcttcaacga agccaccctg ctgtccgcca
     5161 tggaaggcgc cggcaagatg gtggacgacg aggagctgcg cgccgccatg gccggccgcg
     5221 gcctcggcac accggccacc cgcgcccaga tcatcgagaa cctgatcggc gagacctaca
     5281 tcctgcggga cggcaaggag cttgtcccca ccgccaaggc cttctccctg atcacgctgc
     5341 tgcgcggcct gggcacccac gagctgacct cgccggaact caccggcgaa tgggagcaca
     5401 agctggccca gatggagcgt ggcgccctgt cccgcagcac cttcatggag cacatccaga
     5461 ccatggcgcg ggacatggtc gagcgcgcca agcgttacga atccgacacc gtgccgggcg
     5521 acttcgcgac cctgcagacg ccctgtccca agtgcggcgg cgtggtgaag gagaactaca
     5581 agaagttcgc ctgccagggc tgcgactgga gcgcctggaa gatcgtcgcg ggccgccagt
     5641 tcgagattcc cgagatggag accctgctgt ccaccgggcg tgtcgggccg ctgatgggtt
     5701 tccgcaacaa gatggggcgg ctcttcaatg cggagatccg cctcaacgac gaccagcagc
     5761 cggagttcga ttttggccag cccaaggaag acgaggcggc cgaagcggtg gacttctccg
     5821 ctcaggaggc cctcggcacc tgccccaagt gcggtggcgg cgtgtacgag cacggcctcg
     5881 cctacgtctg cgaaaaatcc gtgggccccg gcaagtcctg cgacttccgc tcgggcaaga
     5941 tcatcctgca gcagcccatc gagcgggaac agatgacaaa actccttgcc gatgggcgga
     6001 ccgagctgct caagggcttc gtgtccaccc gcaccaagcg caagttctcc gccttcctgg
     6061 tgcgcggcgc ggacggcaag gtggggttcg aattcgagaa gaaggaaccc cgcgccaagg
     6121 cgggggacgc ggcggacggc gcagcgcccg ccgccaagaa ggcggctcct cgacgcaagc
     6181 cggccgccgg ctgaacgcac gccgcgcagc atccacgccg ggccgcgcgg catgcgccgc
     6241 ccggccccgt cacacgtgga tgacccgcca ggtgccgcct gacttcacgg ccatcaggcc
     6301 gccaggaacg acggcccgcc gcagatcagc ggatggccgc agccacccgc tcgcggaaat
     6361 cgtccagatc ctgatcagag gtgcccagat aggccatcgc ctgctgcgcg aggcccgtgt
     6421 ccagctcgcc gccggtgtcg ccgatgcatt ctgccaacac atgctcggcc accagggtca
     6481 cggccatcat gcccagggct tcgggcgtca ggctgtcgtc gggctttgaa aaaatatccg
     6541 gttcgtggtg gaaacggatc gcggccgcca gcaccggggg cagaccccag ttgcgcgcca
     6601 gcagccatcc gaccgtcgga tggctgcacg ggaagtgggc cagctcgtcc gccaccagcg
     6661 ggcgcccgga ggcctgcgcg gcggcataga cggctccata ctggggatag tgggtcatca
     6721 tcaccgggat ggcggcgtcg tgaaacaggg cgaaggtata ggcattctcg gccgagacgc
     6781 ccatgtggca gcgggcgatc acggccgtgg aaagggccag ggtggccgcc cggttccaga
     6841 acgggtccat cagctcggac ggcaggcttg cactggccga ccgcaaggcc gcggcgatca
     6901 ccacgctcag cacgttgctg atccctagcc ggttgaccgc ctgggacacg ctcttgaccg
     6961 gcgacgtggc acggaacagg gcggaattgg cgagcttgat cgccatggcc gacatgccca
     7021 cgtcggaggc gatcacgcgg gccagcacgc gcatgtccgg gtcgtccttc tgggcctcgc
     7081 gcatcacttc ggcgacaacg gcagggcagg cggggatctg gatcgactgc agggcctcat
     7141 ccaggtcgtc gtgggtcagg gcgggccggg cctgcacggc aggggaagac atgggaactg
     7201 ctcctgtggg gattcggatc gatcggttcg accggtgatt cgtgacgggc tgcgcagagg
     7261 gcccgcaccg actccggcat catggacgcc ccgtgcacgc cgacacagtc ggatacaggg
     7321 gcggacttct cctgaagaaa agtttcggcc ccgcgtggca tttcttgaga gtccggtggc
     7381 ataaaactta tctccccggg cattcaagaa tctgataccc gtgaccgaga aagaccttga
     7441 ttcatccatg ggattaaagt cgtggtcacc catgccgttc attggggcgc catgtcgagg
     7501 aatgtcaccc tgcgcaggcc cgggcgtccc gtcccggttc cggagatcga gcaatgaccg
     7561 catcgccatc gtcgcccgca gccctggtct cgctgaagca ctgccgcgac cgtttcgtgg
     7621 acgccatccg ggcggccgcg cgcacggccc gctgtccgga accctggctc gacggcctgt
     7681 gctccgcggc cgcagagggt ttcgaggaaa tggcgggtct gcgcgagcgg cgcggctttg
     7741 aacagctccg cggcctgacg gcgtcacgga tcagcctgat ccaccaggaa gacctcgact
     7801 actccatcga gctcatcaat ctcgagcagc gcatccacga ccacgtccat cgggagatga
     7861 ccctcgcgga ccggcgcctc catgagctct tgatcgaact gaacctcacc cgcggcgagg
     7921 attcgccctt cggcagtgaa gtggcctgcc tggccctgcg ggcgctgacc gagcgggaac
     7981 gcatcaaccc ggtggaagcc ctcgatttcg cgcgccagat cgaagccccc ctcaagatcc
     8041 agctcatcga actctaccgg gacgtggacc acaccctcgc cggtgtgccg tcctacggcc
     8101 agaagtccgg tcgcgcgccc gcgccacgcc ccggcacccc ggacggccag gtgctgagca
     8161 tcggggacgg cggccacgat ccggtggacg gtctgcgctg ggccgtgatc tcccgggccg
     8221 gcgacggact cagtgagacc gacggccagg accgcgccgc gctgaaggcc gtggtggatc
     8281 gcatcgaggc gtggatggac cacgaacgcc agaacggcga gtgggcccac gacgaactga
     8341 gcacctcgga actcgtgcaa ctcctgccgc cgccccgcgt ggcggccctg gccgtggtgg
     8401 aggcggtgtt cgcccatgcc ctcacccgcg ccgatctgcc cggcccgatc cgcgccctgc
     8461 tggtggaatt gcgcttcccc atcctcaagc aggccctgcg ccacacggat ctgttcgagg
     8521 gctcctcgcg ccctccggcc ttgcgcctgg tggacagcat cgcccagctt ggcgccagcc
     8581 tgccgcccga aggtaccgcc gaccactccc tgtaccgcgc cgcggccaag gtggtgaacg
     8641 cccttgtcca gcctgacact ctgcccgacg acgccttcgc ggtggccctc gaagccctcg
     8701 acaagcgggt cgcggagcgc cgccagacga ccctggagcg cgcccgcgcc ttcgaagaga
     8761 agggcgttca cctggagcgc cgcgagatcg cgctgcacca ggccacacgc atggttagcc
     8821 agctcctcac ccaggcgccg gaaggcccgg tgcgccagtt cctcgaaggg tactgggtcc
     8881 aggtgctgac ccgcgccgcc tacctgcgcg gcaccgatca cgcggaatgg gactcacggg
     8941 tcagaacggc cgcacagctc attgcgagca tcgtcccacc ggccgatgac gcggaacggg
     9001 aacgcctgcg cgccggcctg ccggccctgc gcggcggtct gggcgatggc ctacaggccg
     9061 tcggactcaa gcccgagacc atcgacgccg ccctggcgcc cctcgacgcc ctccacgccg
     9121 cagtgctcga tggcaggctc ctgcccgcca tcccgcccaa accacgcact ccacgggtcc
     9181 accacagccc ggtgagcggc tttccggggc tgtttgccct cgcccacaag tcctatctgg
     9241 ccggcgaacc ggtgctggcc gccgaatggg ccgacatccg cacgggcgac gccctggtcc
     9301 tcgccctgcc ggacggcggc acgctgcgcg gcctggtggc ctggatcggc cctggcgggc
     9361 agctgatgct gatcgccgac accgaccgcc acgatgtgct ggcggtcacc ttccgcgcct
     9421 tcgcccagca gaccgcccag ccgggcagcc ggcgcctgcg ccccttctcc ctggtggagg
     9481 acgctgccga ccggcggctc gcccacgcct gaccccggtc ggccgggaac ccggccgcgc
     9541 ggcggcggtc tgcccgaggg cgctacaatc gccccggaca acccagccct cgccctgcca
     9601 tgtgctccga tcccgcctgc acggccgccc ccccggccgg ctccgatccc gacgccgcca
     9661 tccgcatccg cggggcgaac cagaacaacc tcaagaacct gtccctggac atccccctcg
     9721 acgagctcgt ggtggtgacc ggcgtgtccg gttcgggcaa gagttccctg gtcttcgaca
     9781 ccctctacgc cgaaggccag cgccgctacg tggagacctt ctccccctac gcacgccagt
     9841 tcctcgaccg catggacaag ccgcgggtgg accgcatcga cggcgtgccg ccggccatcg
     9901 ccatcgacca gaccaacccg gtacgcacct cccgctccac cgtgggcacc atgaccgagc
     9961 tggccgacca cttcaagctg ctctacgccc gcggcgcacg cctgcactgc cgcggctgcg
    10021 gccggccggt gcggcgcgac accccggcca gcatccacgc cacccttgcc gaacgggccg
    10081 ccgccgcggg cgatccgcgc ctggtggtga gcttcccggt gagcgtcccg gccgccttca
    10141 gcgaggccga gatcgccgaa ctcctcgccc gccagggcta cacccgcgta caggcccgcg
    10201 acggcgacgt gcgccacgtg gtgcaggacc gcttccgcct gggcaacgcc gagcacgccc
    10261 gcgtgatgga agccctcgaa accgccctgc gcgccggcca cggccgcctc accgtgcacg
    10321 ccctggggga cgccggcgag gcgcgctggc ccttcagcgc cggcctgcac tgcgccgagt
    10381 gcgacatcgc ctacgccgac ccggtgccca gcctgttctc cttcaactca cccctcggtg
    10441 cctgcgacac ctgccgcggc ttcggccggg tgatcggcgt cgatttcggc ctggtcatcc
    10501 cggacgagac cagaaccctc gccgaaggcg ccgtcaaacc ctggcagagc cccagcttca
    10561 aggaatgcca cgacgacctg gtgaagatgg ccggcaagta cggcgtgcct ctggacgtgc
    10621 cggtggccga cctgtcgccg gaacaccgcc agtggctgct ggaaggcgac gcccgctgga
    10681 agagctggga cagctcgtgg ccgcgctact ggtacggcgt gcgccacttc ttcgactggc
    10741 tggagagcaa ggcctacaag atgcacatcc gggtgctgct gtcccgctac cgcagctaca
    10801 ccgaatgccc cgcctgccac ggcgcccgcc tcaagcccga cgccctgctg tggc
//
LOCUS       DAOQPA010000113        10808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3165_length_10808_cov_16.820608, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000113 DAOQPA010000000
VERSION     DAOQPA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_10808_cov_16.820608"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..638)
                     /locus_tag="PKX01_13805"
     CDS             complement(75..638)
                     /locus_tag="PKX01_13805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ77251.1"
                     /translation="MMRPTVLRLLVSLGLALAAASGHAADACPSLLDHTLPRLQDDKP
                     QSLCDYRGKVVLIVNTASYCGFTGQYDGLEKLYARLQPRGLVVLGFPSNQFGNQEPGS
                     NKEIAEFCRLTYGVKFPMFAKTEVIGSAAHPLFRQLASLTGERPKWNFHKYLIARDGR
                     TVRSFPSTTEPTDSTFVAAIEDALGRP"
     gene            complement(635..844)
                     /locus_tag="PKX01_13810"
     CDS             complement(635..844)
                     /locus_tag="PKX01_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77252.1"
                     /translation="MKSSVALLPPLAAAGVLLVGLCQCLGAAVAASAPCGAEACPVQA
                     APRVPDAAAELDVKLFLHTLSPGAR"
     gene            929..1846
                     /locus_tag="PKX01_13815"
     CDS             929..1846
                     /locus_tag="PKX01_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ77253.1"
                     /translation="MNTPAPLPGIPIAAVERDTGLSKDLLRVWERRYGFPTPLREAGG
                     ERSYPPEQLEKLRLIKRLIDHGLRPARLMGASTETLAGLLDGCAAPGGADRCAEFEAL
                     LRRVRLRHHVELRCTLQQMLLKHGLQRFVCEVVAPMNARIGDAWLRGEIEIADEHLYT
                     EHVQNLLRTAIGTQPAAGLQRPRVLLTTFPDERHALGLLMVEAMLTSEGAACLSLGTQ
                     TPVRDIAGAAAAGEADIVALSFSIAHPLRPALDGLRALREALPAKVDLWVGGAGVCGR
                     QDRLPGIRVIERIEDCVDALGVWRAAHPA"
     gene            complement(1923..2387)
                     /locus_tag="PKX01_13820"
     CDS             complement(1923..2387)
                     /locus_tag="PKX01_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ77254.1"
                     /translation="MIDQNRPQALREAIELFFFAYRTFTAPPDRILAERGLGRVHHRI
                     LYFVGRNPDIAVSALLGVLGVSKQALNAPLRDLQAQGLVTATAAAHDRRVKQLALSPA
                     GAALEAELSGTQMAMLEAVFGAAGPQAEAAWRTVMASLVESARPEGARPPFP"
     gene            2510..3691
                     /locus_tag="PKX01_13825"
     CDS             2510..3691
                     /locus_tag="PKX01_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HMZ77255.1"
                     /translation="MTSPSPTASIPRSPAAAPVLSTRVADLHPSPIREILAVAGQPGM
                     ISFAGGLPAAETFPRFGVAAPDTVWQYGPTEGDPALRARIAVELGALGVDAAAERVLV
                     LSGSQQGIDLVGKLFVDAGTPVAVESPTYLAALQVFRFFGARFRPFAPDSLSAADWQS
                     DRPTFAYAIPTFQNPTGRCYSAAERIALAAACRDAGVPLFEDDPYRDLVYDACERRPV
                     CAELAGGTWIYQGSFSKSLAPGLRLGYLAASADLFPYLVRLKQAADLHSNRLSQWLVG
                     EQLDAPERPQRLARLADFYRAKRDAFEAALRRHFGALADWHTPAGGLFFWLTLKRPVD
                     TRLLLPAALARGVAFMPGEAFYPDAPGATGTLRLNFSHAAEADMDRGLAVLAELVAAA
                     E"
     gene            complement(3745..4539)
                     /locus_tag="PKX01_13830"
     CDS             complement(3745..4539)
                     /locus_tag="PKX01_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HMZ77256.1"
                     /translation="MIRNRFKERLRAGELLYGFWLGLSEPFTAEICAGAGFDWLLIDG
                     EHGPNDLRTIFAQLQAIAPYASAPVVRPPQGDHVLIKQLLETGVQTLLIPMVESAAQA
                     RHLVEAMHYPPEGIRGVGSSIARASRWGRIADYSARANAEMCLLVQVETRAGMDALDE
                     ILAVPHVDGVFFGAADLAASYGLLGQPNHPDIVARIEAGLRAARARDMPGGVLCGDPA
                     LVQRYRDAGARFIAIGVDSMLLAAATSKLLDAYRSGHFDGLTGSAY"
     gene            complement(4654..5298)
                     /locus_tag="PKX01_13835"
     CDS             complement(4654..5298)
                     /locus_tag="PKX01_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ77257.1"
                     /translation="MSGSAPMPLRDGAERVYVVDDDLSVREAVCSLIRSVGLRVETFP
                     SAAAFLAAPRDDGPACLILDVRMPGLSGLELQTELHRRGTSLPIIFVTGHGDIPMAVH
                     TIKKGAVEFLSKPFRDEDLLAAIDVALAQARSACSAGRELGEIRRRYAQLTAREKEVI
                     AFMVKGALNKQAAAELGVSEMTVKVHRRNVMHKMGVARLPDLVRMMERLQGAGG"
     gene            complement(5295..5711)
                     /locus_tag="PKX01_13840"
     CDS             complement(5295..5711)
                     /locus_tag="PKX01_13840"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HMZ77258.1"
                     /translation="MTDTTLTGGCACGAVRYTSTAAPVLTAHCHCRDCQRASGAAYAT
                     VCAVPRAAFRVDQGETRSYRSIGDSGHGVVRHFCPTCGAPLFSDVEVAPDLSFLRVVS
                     LDDPAVVTPTAHVYCASAQPWDTHPDGLPAFPRMPG"
     gene            complement(5755..8646)
                     /locus_tag="PKX01_13845"
     CDS             complement(5755..8646)
                     /locus_tag="PKX01_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77259.1"
                     /translation="MRPAECKDRVTARTPVHGVVIEPTDSAEVSRQKMARIILDSMYQ
                     FLGLLDVDGTVLEINRAALDGAGLDLADVIGQPFWHARWWAVSEEVRQRVRDMIAEAR
                     SGRFVRCDFEVFGDAHGSRTIVIDFSLTPILDDAGQVAYLLPEGRNITEKIARNAELS
                     RKNGELQVALERLKELDGYKTRFFANVSHELRTPLALILGPVDQLIQDGQALAERERF
                     RLAAIQRNARALLQQVNNLLDLARIDADRMPMAYVHANLAALLREVAAGFDAVAEDRG
                     IRVSLRGEDELYADVDRAKFMRVLSNLLSNAFKFTPPGGRIGCSVERLAGGRVLVSVQ
                     DTGPGVPAAMKAAIFDRFVQGGDGLGPGGSGLGLNIVKEFVELHRGTVVVLDAPGGGA
                     LFQVELPARAPEGAFVRSAGRDAAPADLAAPLAPELPPLTELPFHPGKRRVLVAEDNP
                     DLRQFLYDVLSDEYNVILRDDGEAAFRAALADPPDLIITDLMMPRWDGERFVRALAEQ
                     PDFPPVPVLVLSARADDALRERLLEHLVQDYLIKPFSARELRARARNLVTVKRTVDIL
                     QKELNSQASDILELTAGLVESRKSLQRSLTALQASDRRWLGLYDNTAVGIALADRDGR
                     IVSANPALQRMLGYGREDIVGVSLIDITEASERARTRQNVEGLFGGGNADYSVQKRYE
                     KRDGGYLWAHVVASRIPAAAEDGPLLAVIVEDITAHMEAERSLAATRAELARVARFTA
                     MGELVASIAHEINQPLSAVITNSQAALRWLSSPADAHLDEVAAALRRVNRDASLAGAV
                     IARIRNFLRAGDLRREPIDVARMLDELLQMLQLMLTDARVRVVVELPEPPPIVCADPV
                     QLQQVILNLTVNAVDAMREVQDRERLLTLSVHRDPAAGLRFDFRDSGAGVAEGTEARL
                     FDALYTTKPCGLGMGLAISRSIVENHGGRLWLDRKAGPGACFAFTIPDG"
     gene            complement(8751..10154)
                     /locus_tag="PKX01_13850"
     CDS             complement(8751..10154)
                     /locus_tag="PKX01_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HMZ77260.1"
                     /translation="MNDARPPLPAPPRPVRRAAAALALAAVAGSAQATDVFRLEGFGA
                     VSRSMGGVATAQDVGPAGMMTNPATLSLLQAGHTLMGGLDLVTTDIEVQDQATGARVS
                     SGHHGSNRGPYVAPELAYAQRVGAWTFGVGAFAQGGLGTEYGSSSFLSRATGGLDTGL
                     DNSSRLLVLNIPLAASFKVNEALTVGAGIDAMWQGLNLNLLLGADQVGSLIGASRVSG
                     ALLPVLGGLPDLRGAHFSLTRNSPLASGINAWGHAERIGMTYQLRPDTRLGAAFTTRS
                     HMADMEGRATLTAVDGVAGQIPLNGNLKIRDFQMPAVLNLGVSHTVSERLQVAADLSR
                     VFWSRVMKDIKVGFVSDGGGDLNILLPQDYKDQNVLAVGAAYQWDTRWTWRGGFRIAD
                     QALRGSTLFAVIPATPRKHLSAGFGYAVSPTASVDLAWSHAFREHLDNTSLPNTSAPI
                     GVNHAQDNATIAFTWRF"
     gene            complement(10183..>10808)
                     /locus_tag="PKX01_13855"
     CDS             complement(10183..>10808)
                     /locus_tag="PKX01_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ77261.1"
                     /translation="DFLPGQYAMLDLPGVGASRAYSLSNTPNDHGEWHFQIRRVAGGR
                     GTAVLFDTLRPGQEVGLDGPYGVAYLRPDAPRDLVCVAGGSGLAPMVSIARGAARAGL
                     LKTRRLHFFYGARTPRDVCGQDFLAALSGFGERVTYTPVVSAPEGTDWDGATGFVHSH
                     MAAALPGPLSRYEFYFAGPPPMTQALQELLMVDHRVPFEQIHFDRFF"
BASE COUNT         1553 a         3720 c         3991 g         1544 t
ORIGIN      
        1 ctgctggggc gcacggcggg agtttgactg accgttggca gacccaaccc acgccccaca
       61 ccgcccccaa cggatcaagg ccgccccagc gcgtcctcga tggccgccac aaaggtgcta
      121 tcggtcggtt ccgtggtgga cggaaagctg cgtaccgtgc ggccatcccg ggcgatcagg
      181 tatttgtgga agttccactt ggggcgctcg ccggtgaggg acgcgagttg gcggaacagg
      241 ggatgggcgg cgctgccgat gacttcggtc ttggcgaaca tgggaaactt gacgccgtag
      301 gtgaggcggc agaactcggc gatctccttg ttgctgccgg gctcctggtt gccgaactgg
      361 ttggacggga agccgagcac caccaggccg cggggctgca ggcgcgcgta gagcttttcg
      421 aggccgtcgt actggccggt gaagccacag tagctggcgg tgttcacgat gagcacgact
      481 ttgccgcggt agtcgcagag ggactggggc ttgtcgtcct gaaggcgcgg cagggtgtgg
      541 tcgagcaggc tcgggcaggc gtcggctgcg tggcccgacg cggcggccaa ggccagcccc
      601 aggctcacga gaaggcgaag gacggtcggt cgcatcatcg ggctcccggc gacagggtgt
      661 ggaggaagag cttcacgtcc agttcggcgg cggcgtccgg gacacggggc gcggcctgga
      721 cggggcaggc ctctgcgcca cagggtgccg aggccgcgac ggccgcgccg aggcactgac
      781 acaggcccac cagcagcacg ccggccgcgg cgaggggtgg gagaagggcg acactcgatt
      841 tcatgatgtt tgtcctggac atgaatggga cacattccta agctagaagc ctgtcagccc
      901 aatgtcaaca atattgtcca ggacaaacat gaacacgcct gcacccctgc cgggcattcc
      961 gattgccgcc gtcgagcggg acacgggtct ctcgaaggat ctcctgcggg tctgggagcg
     1021 gcgctacggt tttccgaccc ccttgcggga ggcgggcggc gagcgcagtt atccgccgga
     1081 gcagctcgag aagctgcgcc tgatcaagcg cctgatcgac cacggcctgc gcccggcgcg
     1141 gctgatgggc gcctccaccg agacgctcgc cggcttgctc gacggctgtg ccgcccccgg
     1201 cggagccgac cgctgtgccg aattcgaggc cctgctgcgc cgcgtgcgtc tgcgtcacca
     1261 cgtggaactg cgctgcacgc tgcagcagat gctgctgaag cacggcctgc agcgcttcgt
     1321 gtgcgaggtg gtggcgccga tgaacgcccg catcggcgac gcctggctgc gcggcgagat
     1381 cgagatcgcc gacgagcatc tgtacaccga gcatgtccag aacctgctgc gcaccgccat
     1441 cggcacccag ccggcggccg ggctgcagcg gccgcgcgtc ctgctgacca cctttcccga
     1501 cgaacgtcat gccctcggcc tgctgatggt tgaagcgatg ctcacctcgg aaggcgcggc
     1561 ctgcctgtcc ctgggcacgc agacgccggt gcgggacatc gccggtgcgg ccgcggccgg
     1621 cgaggcagac atcgtcgccc tgtccttcag tatcgcccat ccccttcggc cggccctgga
     1681 tggcctgcgc gccctgcgcg aggcgctgcc ggcgaaggtg gacctgtggg tcggcggcgc
     1741 cggcgtatgc ggccgtcagg acaggctgcc gggcatccgg gtgatcgagc gcatcgagga
     1801 ttgcgtggac gctctgggcg tctggcgcgc cgcccacccg gcctgaacgg cccacatcgc
     1861 cgcgtcgtcc ccgcgccacc cgaggcgccc gacacgcacg cagggcgtcc cgttggccat
     1921 cctcagggga atggcgggcg ggccccctcc ggccgggccg attccaccag gctcgccatg
     1981 accgtccgcc aggccgcctc ggcctgcgga ccggccgcgc cgaacacggc ctccagcatg
     2041 gccatctggg tgccgctgag ctccgcctcc agcgccgcgc cggccgggct cagggccagc
     2101 tgcttgacgc gccggtcgtg ggccgccgcc gtggcggtga cgaggccctg ggcctgcagg
     2161 tcccgcagcg gtgcgttgag ggcctgcttg ctgaccccca gcacccccag cagggcgctc
     2221 accgcgatgt ccgggttgcg ccccacaaag tacaggatgc ggtgatggac gcgcccgagg
     2281 ccgcgctcgg cgaggatgcg gtccggcggc gcggtgaagg tccggtaggc gaagaagaag
     2341 agctcgatgg cctcgcgcag ggcctgggga cgattttggt caatcatgtt gacttataac
     2401 ggtcggacgc ctaggattta tggtcaagtt tattgactta tctgtccggc ggcaatggca
     2461 tccacccctg cgccgccacc cgatccccca agccgaaccg gagcccacga tgacgtcccc
     2521 ttccccgacc gcctcgatcc cccgatcccc cgccgccgcg ccggtccttt ccacgcgcgt
     2581 ggcagacctg cacccgtcgc cgatccggga gatcctggcc gtcgccgggc agccgggcat
     2641 gatctccttt gccggcggcc tgcccgcggc agagaccttt ccgcgcttcg gcgtggccgc
     2701 gccggacacc gtgtggcagt acggcccgac ggaaggcgac ccggccctgc gggcgcgcat
     2761 cgccgtggag ctcggggccc tcggcgtgga tgcggcggcg gagcgggtgc tggtgctgtc
     2821 cggctcccag cagggcatcg acctggtggg caagctgttc gtggatgcgg gcaccccggt
     2881 ggcggtggag tcgcccacct acctggccgc cctgcaggtg ttccgcttct tcggcgcgcg
     2941 ctttcggccc ttcgcgccgg acagcctgag cgcagccgac tggcagtcgg accggccgac
     3001 cttcgcctac gcgattccca ccttccagaa ccccaccggg cgctgctaca gcgccgccga
     3061 gcggatcgcc ctggccgccg cctgccgcga cgcgggcgtg cccctgttcg aggacgaccc
     3121 ctaccgggat ctggtctacg acgcctgcga gcgccgcccg gtgtgcgccg agctggccgg
     3181 cgggacgtgg atctaccagg gctccttctc caagagtctc gcgccgggcc tgcgcctggg
     3241 ctatctggcg gcctcggccg acctgtttcc ctacctggtg cgcctcaagc aggcggccga
     3301 cctgcacagc aaccgcctga gccaatggct ggtgggcgaa cagctcgacg cgccggagcg
     3361 cccccaacgc ctggcccgcc tggcggactt ctaccgggcc aagcgggacg ccttcgaagc
     3421 cgccctgcgc cgccacttcg gcgccctggc ggactggcac acgccggccg gcgggctgtt
     3481 cttctggctc accctcaagc gcccggtgga cacccgcctg ctgctgccgg ccgccctcgc
     3541 ccgcggcgtc gccttcatgc ccggcgaggc cttctacccg gacgcgccgg gcgccaccgg
     3601 caccctgcgc ctcaacttca gccacgccgc cgaggcggac atggaccggg gactggcggt
     3661 gctggcagaa ctggtcgccg cggccgagtg acgctgcggc ggtccgcgcg cctgtaacgg
     3721 acgccgcgag tcgcgcggcc cggctcagta agcgctgccg gtcagcccat cgaagtgtcc
     3781 actccgatag gcgtccagca acttcgaggt ggccgcggcg aggagcatgg aatccacgcc
     3841 gatggcgatg aagcgggcgc ccgcgtcccg gtagcgctgc accagggccg ggtcgccgca
     3901 cagcacgccg ccgggcatgt cccgcgcccg ggcggcgcgc aggccggcct cgatgcgcgc
     3961 cacgatgtcc gggtggttgg gctggccgag caggccgtag gaggcggcca gatcggcggc
     4021 gccaaagaac acgccgtcca cgtggggcac cgccaggatc tcgtccaggg cgtccatgcc
     4081 ggcgcgggtc tccacctgca ccagcaggca catctccgcg ttggcgcggg cgctgtagtc
     4141 ggcgatgcgc ccccagcggg aggcccgcgc gatggagctg cccacaccgc ggatgccctc
     4201 cggcgggtag tgcatggcct ccaccaggtg acgcgcctgg gccgccgact ccaccatggg
     4261 gatcagcagg gtctgcacgc cggtctccag cagctgcttg atgagcacgt ggtcgccctg
     4321 gggcgggcgc accaccggcg cggaggcata gggcgcgatg gcctggagct gggcgaagat
     4381 ggtgcgcagg tcgttggggc cgtgctcacc gtcgatgagc agccagtcga agccggcgcc
     4441 ggcgcagatc tcggcggtga agggctcgga cagaccgagc cagaagccgt agagcagctc
     4501 gcccgcgcgc aggcgttcct tgaagcggtt gcggatcatc tccaccctgt catcctcctg
     4561 atcgttcctc ttgttgccgg acgggccgcc acccggcgtc gcgccacgac tgtagcggca
     4621 aacgccgacg ccgtcgcgcg ggccggcccc ccctcagcca ccggcgccct gcagacgctc
     4681 catcatgcgc accagatcgg gcagccgcgc cacgcccatc ttgtgcatga cgttgcggcg
     4741 gtgcaccttg acggtcatct ccgacacgcc cagctcggcc gccgcctgct tgttgagggc
     4801 gcccttcacc atgaaggcga tgacctcctt ctcgcgggcg gtgagctggg cgtagcggcg
     4861 gcggatctcg cccagctcgc gtccggccga acaggcgctg cgggcctggg ccagggccac
     4921 gtcaatggcg gcgagcaggt cctcgtcgcg gaagggcttg gacaggaact ccaccgcccc
     4981 cttcttgatg gtgtgcaccg ccatcgggat gtccccgtgg ccggtgacga agatgatggg
     5041 cagcgaggtg ccccggcggt gcagctcggt ctggagctcc agaccgctga ggcccggcat
     5101 gcgcacgtcg aggatcaggc aggccggtcc gtcatcccgc ggcgcagcga gaaaggcggc
     5161 ggccgacggg aaggtctcga cgcgcaggcc caccgagcgg atcaggctgc acacggcctc
     5221 gcgcacggag aggtcgtcgt ccaccacgta cacccgctcg gccccgtcgc ggagcggcat
     5281 cggcgcgctg ccgctcatcc gggcatccgc ggaaaggcgg gcaggccgtc cgggtgggta
     5341 tcccagggct gggcgctggc gcagtagacg tgggccgtgg gggtgacgac ggcgggatcg
     5401 tccaggctca cgacgcgcag gaagctcagg tccggcgcca cttccacgtc ggagaagagc
     5461 ggcgcaccgc aggtcgggca gaagtgccgc accaccccgt ggccgctgtc gccgatggag
     5521 cggtagctgc gcgtctcgcc ctgatccacg cgaaaggccg cgcgggggac cgcacacacg
     5581 gtggcgtagg cggcgccgct ggcgcgctga cagtcgcggc aatggcagtg ggcggtgagc
     5641 accggcgcgg ccgtgctggt atagcggacg gcaccgcagg cacagccgcc ggtgagggtc
     5701 gtgtcggtca tggggatctc ctcggttcag ggtcggcgcg ggcgcgggac ggtgtcagcc
     5761 gtccgggatc gtgaaggcga agcacgcgcc cggccccgcc ttccggtcga gccacaggcg
     5821 gccgccgtgg ttctcgacga tgctgcggct gatcgccagg cccatgccca ggccgcaggg
     5881 tttggtggtg tacagcgcgt cgaagagccg cgcctcggta ccctcggcca cgccggcgcc
     5941 actgtcgcgg aagtcgaagc gcagcccggc cgccgggtcg cggtggacgg agagggtgag
     6001 caggcgttcc cggtcctgca cctcgcgcat ggcatcgacg gcattgacgg tgaggttgag
     6061 gatgacctgc tggagctgca ccgggtcggc gcagacgatc ggcggcggct cgggcagctc
     6121 gacgacgacg cgcacgcggg catcggtgag catgagctgg agcatctgca gcagctcgtc
     6181 gagcatgcgc gccacgtcga tgggttcgcg gcgcaggtcg ccggcgcgca ggaagttgcg
     6241 gatgcgggcg atcaccgcgc cggccaggct ggcgtcccga ttgacgcggc gcagggcggc
     6301 ggccacctcg tccaggtggg cgtcggccgg gctggacagc cagcgcaacg cggcctggct
     6361 gttggtgatg accgccgaca ggggctggtt gatctcgtgg gcgatggagg ccaccagctc
     6421 gcccatggcg gtgaagcggg ccacgcgggc gagctccgcg cgggtggcgg cgagggagcg
     6481 ctcggcctcc atgtgcgcgg tgatgtcctc gacgatcacc gccagcaggg ggccatcctc
     6541 ggcggcggcg gggatgcgcg aggccaccac gtgggcccac aggtagccgc cgtcgcgctt
     6601 ttcgtagcgc ttctgcacgc tgtagtcggc gttgccgccg ccgaacaggc cctccacgtt
     6661 ctgacgcgtg cgggcccgct cggaggcctc ggtgatgtcg atcagggaca cgccgacgat
     6721 gtcctcgcgt ccatagccga gcatgcgctg cagggccggg ttggcggaga cgatgcgccc
     6781 gtcccggtcg gccagggcga tgcccaccgc ggtgttgtcg tagaggccca gccagcgccg
     6841 gtccgaggcc tgcagggcgg tcaggctgcg ctgcagggac ttgcggctct ccaccagacc
     6901 ggcggtcagc tcgaggatgt cggaggcctg ggagttgagc tccttctgca ggatgtccac
     6961 ggtgcgcttg acggtcacca ggttgcgcgc ccgggcgcgc agctcccgcg ccgagaaggg
     7021 cttgatcagg tagtcctgga ccaggtgttc aagcaggcgc tcgcgcaggg cgtcgtcggc
     7081 gcgggcggac agcacgagca ccggcacggg cggaaaatcc ggctgctcgg cgagggcccg
     7141 cacgaagcgc tcgccgtccc agcggggcat catcaggtcg gtgatgatga gatccggcgg
     7201 atcggcgagg gccgcgcgga aggccgcctc gccgtcgtcc cgcaggatca cgttgtactc
     7261 gtcggagagc acgtcgtaga ggaactggcg caggtccgga ttgtcctccg ccaccagcac
     7321 gcgccgcttg ccgggatgga agggcagctc ggtcaggggc ggcagttccg gcgcgagggg
     7381 cgcggcgagg tcagccggcg ccgcgtcgcg gccggcgctt cgcacaaagg cgccttccgg
     7441 cgcgcgggcg ggcagttcca cctggaacag ggcgccgccg ccgggggcgt ccagcaccac
     7501 cacggtgccc ctgtgcagct cgacgaactc cttgacgatg ttgagaccca ggccgctgcc
     7561 gccgggcccg agcccgtcgc cgccctgcac gaagcggtcg aagatggccg ccttcatggc
     7621 cgccggcacg cccggcccgg tgtcctgcac gctcaccagc acgcggccgc cggctaagcg
     7681 ctcgacactg cagccgatgc gcccgccggg tggggtgaac ttgaaggcgt tggacagcag
     7741 attgctcagg acgcgcatga acttggcccg gtccacgtcc gcgtagagct cgtcctcgcc
     7801 ccgcaggctg acgcggatac cccgatcctc tgccaccgcg tcgaagccag cggccacctc
     7861 ccgcagcagg gccgccaggt tggcgtgcac gtaggccatg ggcatgcggt cggcgtcgat
     7921 gcgggccagg tcgaggaggt tgttcacctg ttgcagcagg gcgcgggcgt tgcgctggat
     7981 cgcagcgagg cggaagcgct cccgctcggc gagggcctgg ccatcctgga tgagctgatc
     8041 caccgggccg aggatcaggg ccaggggcgt gcgcagctcg tggctgacgt tggcgaagaa
     8101 gcgggtcttg tagccgtcca gctccttgag gcgttcgagg gcgacctgca gctcgccgtt
     8161 cttgcgcgac agctcggcgt tgcgggcgat cttctcggtg atgttgcgcc cctccggcaa
     8221 caggtaggcg acctgcccgg cgtcatccag gatgggcgta agggaaaagt cgatgacgat
     8281 ggtgcgactg ccatgggcgt cgccgaagac ctcgaagtcg cagcgcacga agcgcccgct
     8341 gcgggcctcg gcgatcatgt cgcgcacgcg ctggcgcacc tcttcggaga cggcccacca
     8401 gcgggcgtgc cagaagggct ggccgatcac gtcggccagg tccaggccgg cgccgtcgag
     8461 ggcggcccgg ttgatctcca gcacggtgcc gtccacgtcc agcaggccga gaaactggta
     8521 catggagtcg aggatgatgc gcgccatctt ctgacgcgac acctccgcgc tgtcggtcgg
     8581 ttcgatgacg acgccgtgca ccggcgtgcg cgccgtcacc cggtccttgc attcagcggg
     8641 tctcatgtcg tgtgctcgtc tcctcgccct gcgccgcctc gggacgtgcg cagaccgggc
     8701 caccgcgcac cctcgcgcag gcgcccgacc cgccctgcaa gcatgcgcct tcagaatcgc
     8761 caggtgaagg cgatcgtggc gttgtcctgg gcatggttca cgccgatggg cgccgaggtg
     8821 ttgggcaggc tggtgttgtc cagatgctcc cggaaggcgt gggaccaggc gagatccaca
     8881 ctggccgtcg gcgacaccgc gtagccgaag cccgccgaca ggtgcttgcg cggcgtggcc
     8941 gggatcaccg caaagagggt cgagccgcgc agcgcctggt cggcgatgcg gaagccgccg
     9001 cgccacgtcc agcgcgtgtc ccactggtag gcggcgccca cggcgaggac gttctggtcc
     9061 ttgtagtcct ggggcagcag gatgttcagg tcgccgccgc cgtcggacac gaagcccacc
     9121 ttgatgtcct tcatgacgcg ggaccagaac acccgggaca ggtccgccgc cacctggagc
     9181 cgctccgaca cggtgtggct gacgcccagg ttgagcaccg ccggcatctg gaagtcgcgg
     9241 atcttcaggt tgccgttgag gggaatctgg cccgccacgc cgtccacggc agtgagcgtg
     9301 gcacggcctt ccatgtcggc catgtggctg cgggtggtga aggcagcccc caggcgcgtg
     9361 tcgggccgca gctggtaggt catgccgatc cgctcggcgt ggccccaggc gttgatgccg
     9421 ctggcgaggg gtgaattgcg ggtcaggctg aagtgggcgc cgcgcaggtc cggcagaccg
     9481 cccagcacgg gcagcagggc gccggacacc cgcgacgcgc cgatcaggct gcccacctgg
     9541 tccgcgccca ggagcaggtt gaggttgagg ccctgccaca tggcgtcgat gccggccccc
     9601 acggtgagcg cttcattgac cttgaagctc gccgccagcg ggatgttcag caccagcagg
     9661 cggctggaat tgtcgaggcc ggtatcgagc ccgccggtgg cgcgggacag gaagctgctg
     9721 ctgccgtact cggtgcccag cccgccctgg gcgaaggcgc cgacgccgaa ggtccaggcg
     9781 ccgacacgct gggcgtaggc cagctccggc gccacgtaag ggccccggtt gctgccgtga
     9841 tggccggagg acacgcgcgc gccggtggcc tggtcctgca cctcgatgtc ggtggtcacc
     9901 agatcaagcc cgcccatcag ggtgtgcccg gcctggagca gggacagggt ggccggattg
     9961 gtcatcatgc cggctggtcc cacgtcctgg gcggtggcga cgccgcccat gctgcgggac
    10021 acggcgccga agccttccag acggaagacg tcggtggcct gggcggaacc ggccaccgca
    10081 gcgagggcca gggccgcggc ggcgcgccgg acagggcgcg gcggcgccgg aaggggtggg
    10141 cgtgcgtcgt tcatggatgt ctccccgtgt agtgttgtgc gatcagaaaa agcggtcgaa
    10201 atggatctgc tcgaagggca cccggtgatc caccatgagc agctcctgga gggcctgggt
    10261 catgggcggc gggccggcga agtagaactc gtagcggctc agcggaccgg gcagggccgc
    10321 ggccatgtgg ctgtggacga agcccgtggc gccgtcccag tccgtcccct cgggggcgga
    10381 caccaccggg gtgtacgtga cgcgctcgcc gaagccggac agggcggcga ggaaatcctg
    10441 cccgcacacg tcgcggggcg tccgcgcgcc gtagaagaag tgcaggcgac gggtcttgag
    10501 caggccggcg cgggccgcgc cgcgggcaat ggagaccatc ggcgcgaggc cggaaccgcc
    10561 ggccacgcag accaggtcgc ggggcgcgtc gggccgcagg taggccacgc cgtagggacc
    10621 gtcgaggccc acctcctggc ccgggcgcag cgtgtcgaag agcaccgcgg tgccgcgtcc
    10681 gcccgccacg cggcggatct ggaagtgcca ttcgccgtgg tcgttgggcg tgttggacag
    10741 ggaataggcg cgagaggcgc cgacgcccgg cagatccagc atggcgtact ggccgggcag
    10801 gaagtcgg
//
LOCUS       DAOQPA010000114        10699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3213_length_10699_cov_16.695227, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000114 DAOQPA010000000
VERSION     DAOQPA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_10699_cov_16.695227"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1187)
                     /locus_tag="PKX01_13860"
     CDS             complement(255..1187)
                     /locus_tag="PKX01_13860"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ77262.1"
                     /translation="MDRLEELRVLLEIADGGSLAAGGRRTGRSPAAVSRILAGMERRL
                     GARLVERSTRSNVLTEAGRRLVEHARTLLADYDDALGDVSREASEATGRLRVSAPLVF
                     GRLHLAPLIGDFLVAHPGIHVDLVLSNALVDLREARIDVALRIDHLADSTLVAKRVGE
                     IRQRVVGSPAYLARVGTPCSPDDLAGHHAILHAHEDGVREWAFRLADGRVQRMRPPCR
                     WTVNQSETLIDAAIAGRGLIRVPSYQTVDHIEAGRLRCVLKGYEMPPVPVHLVFASTR
                     FIPLRLRRFLDFTAAALGTSTAFREPGDHTPNTD"
     gene            1317..2105
                     /locus_tag="PKX01_13865"
     CDS             1317..2105
                     /locus_tag="PKX01_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HMZ77263.1"
                     /translation="MSLSRRQFVQAALAGPALALDLPARAEARRVAPIQAVALDGFTT
                     FDPRPVAALAERLFPGQGAALTTAWRTRQFEYSWLRNSMDRYVDFWQVTEEALVFAAR
                     QTRLELDGTRRRQLMQAFLALDAWPDARPALERLHASGIRLAFLSNFTVEMLDAASRN
                     AGLAGLLEPHLSTDRVGVFKPHPRAYRMGVEAFGLPREAIAFAAFGGWDAAGAKAFGY
                     PTFWVNRLGLPVEQLGVQPDGIGASLEDLVRFVSTSNAASRPAA"
     gene            2129..2659
                     /locus_tag="PKX01_13870"
     CDS             2129..2659
                     /locus_tag="PKX01_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HMZ77264.1"
                     /translation="MKSVLVSALCASLSVLSAPVSAQVQVSGRSVPLAIAVDAAAAAL
                     KRCEADGYRVTVSVVDAAGVERVRLRGDHSTIHTSETAFRKAYTVITLGPIFKATTTS
                     ALTERIGKTPNLPALSTIDHVIFLAGGVALQAQGEFLGAIGVGGAPGGALDEQCAQAG
                     VQAVQARLDGLGVPSN"
     gene            complement(2920..3831)
                     /locus_tag="PKX01_13875"
     CDS             complement(2920..3831)
                     /locus_tag="PKX01_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ77265.1"
                     /translation="MNSDTPFLAARQTPAHPAAVRLAHHEVNGTRIFYRSAGDPAQPA
                     IVLLHGFPASSHMFRDLIPHLAGHFHVIAPDYPGFGHSDAPAPGRFPYSFDALAGVVD
                     GLLAHLGVDRYHLYMQDYGGPVGLRLATAHPERVLGLVIQNANAYLEGISPMVRDVFM
                     PLWEQGDEGPARALLDAATTRFQYLTGAGDPLAVSPDAWVFDQALLDRPGNAEIQLAL
                     FRDYRHNVDLYPVWHAYFRQRQPKTLIAWGRGDPLFLVEGAEAFRGDLPAAAVHYLDG
                     GHFVLEEHAARVAELILRHFTPERAAG"
     gene            3987..4616
                     /locus_tag="PKX01_13880"
     CDS             3987..4616
                     /locus_tag="PKX01_13880"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HMZ77266.1"
                     /translation="MSPAPTPFIFVADDVALDFANTLAAPTGTLFDFLGAPGSLTAWA
                     QASGALPALGALLVAAAAHGELAEVEDEARALRDWLVGVLPQIRAGSAPLAAVDLAPL
                     NRALTAARCAWSVHACEGRATLARDWCYAGPLSALAPLAEAIASLLARPDLHDIRKCE
                     NPQCTLWFRDVTKRGHRRWCSTAVCGNRAKVAAFRDRQKRATRETDGDG"
     gene            5058..6824
                     /locus_tag="PKX01_13885"
     CDS             5058..6824
                     /locus_tag="PKX01_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77267.1"
                     /translation="MSSLVDREWLDDEEAARNTCILVVERDEASAEVFQAFLKTSGFG
                     NVVTTLGATAVLDLLYDLEPDLLLVDSGALRAPDGSDLLESIRATRAFRHVPVLVLAA
                     QADREMKLQVLQQGVLDVLIKPVDPAELVLRLRNVLLSKLRGDRATFRDDLTGLANRE
                     RYTDRLQWAIRYSKRYGAEGAVLHIDLDRFKQIVSALGWGVGDRVLRAIANNLAVCVR
                     DTDVIAQGGERERSVMLSRLSGNEFTVLLSGIDRADSAAVVADRILHLVQQPMQVAGR
                     ELITTCSIGIAVFPSDGNSAEEIISAAVNALQHAKREGGNTFRFFARALNDRARHRLA
                     LESDLRVAVDEGQLGLVYQPKVRLDGNRITGAEALVRWIHPQRGVINPMDFIPVAEEC
                     GLIHAVGDWVLRETCRQIRAWREQGLAVPRIAINVSVHQFRQAGFADGVAAVLAEYGL
                     EGSVLGVELTESAIMENTVESLGALQALKDLGIELSIDDFGTGYSSLGYLKRFPLHIL
                     KIDRSFMIGVGKDEHSAAIVSAIISIGHRLGLRVVAEGVDTARQLEFLASHGCDEHQS
                     FLFSRPLYPDDFVAFLKRHTSL"
     gene            7084..8850
                     /locus_tag="PKX01_13890"
     CDS             7084..8850
                     /locus_tag="PKX01_13890"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ77268.1"
                     /translation="MTERSLTSIQRWLESYCGQTAVVAAGVIVRSEGSADAVRMLAAW
                     PAQGDALQPLVGAAAAALTRRQAVTMVPPVALPDTGIVRIVALPIQHTGDAGALAIGL
                     RSAEDSVVQGFLGEVQRLGATLAQLLQAPDAAPLREAGRLLQLQSVLLGRARLAEAAL
                     AFANELAATFAFDRVAVGLVEDGACRIEALSHNADFDRGQDLLRCLAAAMDEAADQGC
                     VLVQPPRPGDRLRIQLAHAELAGRTGAGVCSIPLAHDGEVIGVLTLERVGLAPPLQDE
                     LAQCEHLAALVAPLIALRQQAERPWRARLAAAGRQTWQRIVRGEDPRLRTGALVAGAV
                     LLATLVVPLPYRVGAAARIEGAEQRVLAAPVDGYLRKASVRPGDAVKTGQVLAEMVDQ
                     DLMLEERKWQAELSQHENAFIAAMARADRPGFSISRAKAGEARAQLELVRSQLARMQI
                     VAPIDGLVIEGDLTQSLGAPVKRGDALLTLAPQSRHRLMVEVDERDIGRVAPGADGEL
                     NLAALPGDSLAFRVMRITPVAVQRNGNNVFEVEAAVDGGDALRPGMQGVARIDAGHRP
                     LVWTLTHRAWDWLRLTLFSWGL"
     gene            8852..>10699
                     /locus_tag="PKX01_13895"
     CDS             8852..>10699
                     /locus_tag="PKX01_13895"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HMZ77269.1"
                     /translation="MTASLFSPHWYRVADLRPRLRAQVRPNRQRWRDQLWYVLADQAT
                     GRHHRINETAYRFIGRCDGRRTVQQVWDAVLDADGDAAPTQDEVVELLTRLYESELLQ
                     SERSPDVDALFQRQSERAAGQRRATVNPLAFRLALGDPHRWLVRLDPLAHAVFRPGVF
                     GLWLVLMGVALITAGLHWPELRDHAARHVSSPRYLALLWLCYPVIKALHELGHALAVR
                     RWGGEVHEAGVVLLALVPAPYVDASAASGFLGRGPRAIVGAAGILVELALAAGGLFLW
                     LNTAPGWINDIAFVVMTVGSVSTLLINGNPLLRFDGYHVLCDALDLPNLAPRSTLYWS
                     DLLRRRVLRVAGEPPQMGAGEAKWLRLYAPLSALYRLAVGVSIMLWLGSKWFVLGVVA
                     AAYLAVAMLFRPVVGWVRQSLAAAAPGPALVRAHRGLLLLLGVPGLLLFVVPMPYVSV
                     APAVVWLPDRAQVRPDVDGFVSELPVRDGQNVAPGELLAVLRNDDLAAERERLDSRLQ
                     GLRSEQFRLLLRDPVGAQNQAEEIARTEAELTRADERLAGLQVRAQAGGRLVMPRQAD
                     LPGAFVRKGQVLGYVFPDGDTLVRAAVPERDGHRVRLAGSTARVRLVGGG"
BASE COUNT         1489 a         3576 c         3902 g         1732 t
ORIGIN      
        1 ccggagaaag ccgggaaagc caaggaaatc aaggcttccc ggcttttttt cgtccagagt
       61 agtccgaaat gggcacttgc aatccagggg cattgacggg caaggccgcg cggaattgcc
      121 cccgaatgcc ccggaccgct gccgcgctcc gcgctgcccg gcccggcgag cagtctctca
      181 cgctgcctga cgcgcggtcc gggttcccgt gggcgccggc gcgttgcatc ggctccttgc
      241 acccgtgatc cgggctaatc ggtgttcggc gtgtggtcgc ccggttcgcg aaacgcggtg
      301 ctggtgccca gggcggcggc cgtgaaatcg agaaaccggc gcagacgcaa agggatgaat
      361 cgtgtgctgg cgaagaccag atgcacgggt acgggcggca tttcataccc tttgaggacg
      421 cagcgcagac gccccgcctc gatgtggtca accgtctgat aggacggcac gcggatcagg
      481 ccacgaccgg cgatggccgc gtcaatcagg gtttccgact ggttcaccgt ccaccggcac
      541 ggtggacgca tgcgctggac acgtccgtct gccagacgga aggcccactc ccgcacgccg
      601 tcctcgtggg cgtgcagaat ggcgtgatgg cccgcgagat cgtccggcga gcagggggtg
      661 ccgacgcggg cgaggtaggc ggggctgccc accacgcgct ggcggatctc gccgacgcgc
      721 ttggcgacaa gggtggagtc ggccaggtgg tcgatgcgca gggcgacgtc gatgcgcgcc
      781 tcgcgcagat ccacgagggc attgctgagc accagatcga cgtgtatgcc gggatgggcg
      841 acgaggaagt cgccgatgag gggtgccagg tgcaggcgcc cgaacaccag cggggcgctg
      901 acgcgcaggc ggccggtggc ttcgctggcc tcgcgggaga cgtcacccag cgcatcgtcg
      961 taatccgcca gcagcgtgcg ggcgtgttcc accaggcggc ggccggcttc ggtgagcacg
     1021 ttgctgcgcg tggagcgttc gacgagccgg gctcccaggc gtcgttccat acccgccagg
     1081 atgcggctca ccgcggccgg cgagcggccg gtgcgccgtc ctccggcagc gaggctgccg
     1141 ccgtcggcaa tctccagcag tacgcgcagt tcttcgagtc ggtccatggt tccttccgcc
     1201 ttgtgcaagt ctgagttgca gccgcgccca attcccggcg gcggcctgcc tgcctagtat
     1261 ccaggcacgt ccccggcatg accggtgcgc acccgcccca agctcaggag agcgccatgt
     1321 ccttgtcccg ccgtcaattt gtccaggctg ccctcgccgg tcctgccctc gcgctggatc
     1381 tgccggcgcg cgcggaggcg cgccgggtcg cgccgatcca ggccgtcgcg ctggacggct
     1441 tcaccacctt tgaccctcgc cccgtcgcag cgctcgccga gcgcctgttt ccgggccagg
     1501 gcgccgccct gaccacggcc tggcgcactc gtcagtttga gtactcgtgg ctgcgaaaca
     1561 gcatggaccg ctatgtggac ttctggcagg tgaccgagga ggccctggtc ttcgcggcac
     1621 gacagacccg cctcgaactc gacgggacgc ggcgccggca gctgatgcag gcctttctcg
     1681 cgctggacgc gtggccggac gcgcgtccgg ccctcgaacg tctccacgcc agtggcatca
     1741 ggctcgcctt cctctccaac ttcaccgtgg agatgcttga cgcggccagc cgcaacgcgg
     1801 gcctcgcggg gctgctggag ccccatctga gtaccgaccg ggttggcgtc ttcaagcccc
     1861 atccgcgggc ctaccggatg ggcgtggagg ctttcggcct gccgcgcgag gccatcgcat
     1921 tcgccgcctt cggaggctgg gatgcggccg gcgccaaggc ctttggctac ccgaccttct
     1981 gggtcaatcg gctcggtctg ccggtcgagc agctgggtgt gcagccggac ggtatcggcg
     2041 ccagcctgga ggatctggtg cgtttcgtca gtacctcgaa tgcagcgtcg cgtcccgcgg
     2101 cgtgagatca acccccctgg agaaccccat gaaaagtgtc cttgtttctg cgctgtgtgc
     2161 atccctgtca gtcctttccg cgccggtttc ggcccaggtc caggtgagcg ggcggagcgt
     2221 gcccctggcc attgccgtcg acgccgccgc ggcggcgctc aaacgctgcg aggcggatgg
     2281 ttaccgcgtc accgtcagcg tggtggacgc cgcgggggtc gaacgcgtcc gcctgcgcgg
     2341 cgatcacagc accatccaca ccagcgagac ggccttccgc aaggcctaca cggtgatcac
     2401 cctcggtccg atcttcaagg ccacgacgac cagcgcactc accgagcgga tcggcaagac
     2461 gcccaatctg ccggccttgt ccaccattga tcacgtcatc ttcctggccg gtggtgtggc
     2521 actgcaggcc cagggtgagt tcctgggcgc catcggcgtg ggcggggcgc ccggcggtgc
     2581 gctcgacgag cagtgtgccc aggccggcgt gcaggctgtt caggcccgac tcgacgggct
     2641 gggtgtgccg tcgaattgag tgggcgtccg gacgatcgac cctggtctcc atcgtctgct
     2701 ccggtcctca gccgccgtca ggcggctttt caccgaaaaa cgcccccggc gcccgggtgt
     2761 tcgagggcgc gccgggccgt tcgggtgggg cggcgcgacg cgaagcggct gtctcgtccg
     2821 gtctcgacgt cagccaccgt ccgggccggc gcctcgtcca ggcgtccgga ccggatggaa
     2881 cgcgtggggc cgggcgagcg ccggacgagg aggcgacgat cagccggcgg cacgttcagg
     2941 ggtgaagtgg cggaggatca gttcggccac gcgggcggcg tgctcttcga ggacgaagtg
     3001 gcccccatcg aggtagtgga cggccgccgc gggcaggtca ccgcgaaagg cttcggcgcc
     3061 ttcgacgaga aacagggggt ccccgcgccc ccaggcaatc agggtcttcg gctgccgctg
     3121 gcggaagtag gcgtgccaca ccgggtagag gtcgacgttg tgacggtagt cgcggaacag
     3181 ggccagctgg atctcggcgt tgccggggcg atccaggagg gcctggtcga agacccaggc
     3241 gtccgggctg acggccaggg ggtcgccggc gccggtgagg tactggaagc gggtggtggc
     3301 ggcgtccagc agcgcgcggg ccggtccttc gtcgccctgt tcccacagcg gcatgaagac
     3361 gtcacgcacc atcgggctga tgccttccag ataggcgttg gcgttctgga tcacgaggcc
     3421 gagcacgcgt tcggggtggg ccgtcgcgag gcgcagtccg accgggccgc cgtagtcctg
     3481 catgtagagg tggtagcggt ccacccccag atgagcgagc agaccgtcca cgacgccggc
     3541 cagcgcgtcg aagctgtagg ggaagcggcc gggcgccggc gcgtcggaat ggccgaagcc
     3601 cgggtagtcc ggcgcgatga cgtggaagtg cccggcaaga tgggggatga ggtcgcggaa
     3661 catgtgggat gaggcgggaa agccgtgcag cagcacgatg gccggctgcg ccggatcgcc
     3721 tgccgagcgg tagaagatcc gcgtgccatt cacctcgtga tgggcgagac gaacggcagc
     3781 ggggtgggcg ggtgtctggc gggccgccag gaagggcgta tcggagttca tgggtgtcct
     3841 tgtgtcagga gatgacggga agcgttctcg taaccgtaaa aatgaaaatt aaaggttact
     3901 gacgcaacgc taatggtgtg ctcgtaactt gtcaacgatt tattttgagg ttacattctt
     3961 gtcgaatctg acgtactgag cgtcccatgt ctcctgcacc gactcctttc atctttgtcg
     4021 ccgacgacgt cgccctggat ttcgccaaca ccctggccgc ccccacgggc actttgttcg
     4081 actttctggg cgcgcccggc agcctgacgg cctgggcaca ggcttccggg gcgctgccgg
     4141 ctctgggcgc tttgcttgtc gcggcggcgg cgcacggtga attggccgag gtcgaggatg
     4201 aggcgcgggc gctgcgcgac tggctggtcg gcgtgctgcc gcagatccgc gcaggcagtg
     4261 ccccgttggc agctgtcgac ctcgcgccac tcaaccgtgc gctgacggcg gcccgctgtg
     4321 cgtggtcggt ccacgcgtgc gagggtcggg caaccctggc acgggactgg tgctacgccg
     4381 gtcccctgag tgcgctggcg cccctggccg aagccatcgc cagcctcctg gcccgcccgg
     4441 atctgcacga tatccgcaag tgcgaaaacc cccagtgcac cctgtggttc cgtgatgtga
     4501 ccaagcgcgg ccatcgacgc tggtgcagca ccgcggtgtg tggcaaccgg gccaaggtcg
     4561 cggcattccg ggatcgtcag aaacgcgcga cccgggagac ggacggagac ggctgaccgg
     4621 gcgacgggct ggcgtccttg cgggtgggcg gtgtggcatg gccgtcgggt cgcttcgtgg
     4681 gcctggcgcc tgtgcatgcg ctgtgcagac ggagatgccc gatgacaggg tgggcgctcc
     4741 ggcgggtgaa gccgagcccc catggaccac cgtgatggca ggggcggggt ccgccaggat
     4801 cagccgaggc gggcagggcg atcagtgaac cgggcgagcc ggtccggatg ggcgacggga
     4861 cgcgagggcg tcagacaggg cgtcatcgcc gggcgcgccg tacggctcgt gacgcagggc
     4921 ccacactcgg catcggcccg gttcaagatc ggccggccga acccgttagc gctggaagtg
     4981 agtctgccgt cattgcccgc catgccctgt tccccgatcg attgccgcat cccctccgcc
     5041 tgtcgggaga ttgcccgatg agttcgctgg tcgaccggga gtggctcgac gacgaggagg
     5101 ccgcacggaa tacctgcatc cttgtggtgg agcgggacga ggccagcgcc gaggtgtttc
     5161 aggccttcct caagacgtcc ggtttcggca acgtggtgac gaccctcggc gcaacggcgg
     5221 tgctggacct gctctatgac ctggagccgg acctgctgct ggtggacagc ggtgcattgc
     5281 gggcgccgga tggcagcgat ctgctggagt ccatccgggc gacccgcgcc ttccgtcacg
     5341 tcccggtgct ggtcctggcc gcccaggcgg atcgggagat gaagctgcag gtcctgcagc
     5401 agggtgtgct ggacgtgctg atcaaaccgg tggatccggc ggagctggtg ctgcgtttgc
     5461 ggaacgtgtt gctgagcaag ctgcgcggcg accgggcgac cttccgtgac gacctgaccg
     5521 gcctggccaa ccgggagcgc tacacggacc gcctgcaatg ggcgatccgc tacagcaagc
     5581 gttacggtgc ggaaggcgcc gtgctgcaca tcgacctgga tcgcttcaag cagatcgtga
     5641 gcgccctggg ctggggcgtg ggcgaccggg tgctgagggc catcgccaac aatctggcgg
     5701 tgtgcgtgcg tgacacggat gtgatcgccc agggcggcga gcgggagcgc tcggtgatgc
     5761 tgtcgcgcct gtcgggcaac gaattcacgg tgttgttgag cggcatcgac cgggcggaca
     5821 gcgccgcggt ggtggcggac cgcatcctcc atctcgtgca gcaaccgatg caggtggccg
     5881 gccgggagct gatcaccacg tgctcgatcg gcatcgcggt gtttccgtcg gacggcaatt
     5941 cggcggagga gatcatctcg gcggcggtga acgcgctgca gcacgccaag cgggagggcg
     6001 gcaacacctt ccgcttcttc gcccgtgccc tcaacgaccg ggcgcggcac cggctggcgc
     6061 tggaatccga tctgcgggtg gcggtggacg agggccagct ggggctggtc tatcagccca
     6121 aggtgcgcct ggacggcaac cgcatcaccg gtgccgaggc gctggtgcgc tggatccatc
     6181 cccagcgggg cgtgatcaat ccgatggact tcatcccggt ggcggaggag tgcgggctga
     6241 tccacgccgt gggcgactgg gtgctgcgcg agacctgccg gcagatccgc gcctggcgcg
     6301 agcaggggct tgccgtgccg cgcattgcca tcaacgtgtc ggtgcatcag ttccgccagg
     6361 ccggctttgc cgacggtgtg gcggcggtgc tggcggaata cgggctggag ggcagcgtgc
     6421 tgggggtcga gctcaccgag agtgcgatca tggaaaacac cgtggagagc ctgggcgcgc
     6481 tgcaggcgct gaaggatctg ggcatcgagc tgtccatcga tgacttcggc accggctatt
     6541 cgtccctggg ctacctgaag cgctttccgc tgcacatcct caagatcgac cgctccttca
     6601 tgattggcgt gggcaaggac gagcacagtg cggccatcgt ctccgccatc atctccatcg
     6661 gccaccgcct cggcctgcgg gttgtggcgg agggcgtcga caccgcccgc cagctggaat
     6721 tcctggccag tcacggctgc gacgagcacc agagttttct gttcagccgt cccctctatc
     6781 ccgacgactt cgtcgccttc ctgaaacgtc acacgagcct ttgaggcggc aggggtgtcg
     6841 ggctgcaggc gcatgcagcg ccggcctcaa gcccgtgcga cggatgccga taagcctctg
     6901 ggtgcccggt ccgatgatct ccgcggcgcc gggtgatggc gcgtccgatc cggcggggag
     6961 cccgatcgga ttgccgggac cttaaaacat tggcgccgca tttcaccgct ctcctgcccg
     7021 ccgcacggcg atggcccctt cgaccggaag ggtgccgctg cgccgcacgg gggcctgacg
     7081 gacatgaccg aacgtagtct tacctccatc cagcgctggc tggagtccta ttgcggccag
     7141 accgcggtgg ttgccgccgg cgtgatcgtg cgcagcgaag gcagcgccga cgcggtacgc
     7201 atgctggccg cctggccggc ccagggcgat gccctccagc ccctggtggg cgccgccgcc
     7261 gccgccctga cccgccgtca ggccgtcacg atggtgccgc ccgttgcctt gcccgacacc
     7321 ggaatcgtgc gcatcgtcgc cttgccgatc cagcacacgg gcgatgccgg cgcgttggcc
     7381 atcggcctgc gcagcgccga agactccgtg gtgcagggct tcctgggcga agtccagcgt
     7441 ctcggtgcca ccctggccca gctgctgcaa gccccggacg cagcgcccct gcgcgaggcc
     7501 ggccggctgc tccagctgca gagcgtgctg ctcgggcgtg cccgcctggc tgaagccgcg
     7561 ctggcctttg ccaatgaact ggcggccacc ttcgcattcg atcgggtcgc cgtcgggctc
     7621 gtggaggacg gcgcctgtcg catcgaggcc ctttcccaca atgccgactt tgaccgcggt
     7681 caggatctgc tgcgttgtct ggccgcggcc atggacgagg cggccgatca aggctgcgtg
     7741 ctggtccagc cgccccgtcc gggcgatcgt ctgcgcatcc agctggccca tgcggagctg
     7801 gccgggcgga ccggcgcggg cgtgtgcagc attcccctgg cgcatgacgg cgaggtgatc
     7861 ggcgtgctga ccctggaacg ggtcggtctg gcgccgccct tgcaggacga gctggcccaa
     7921 tgcgaacacc tggcggccct cgtcgcgccc ctcatcgccc tgcgccagca ggctgagcgg
     7981 ccctggcggg cccgcctggc cgccgccgga cggcagacgt ggcagcggat cgtccggggc
     8041 gaggacccac gcctgcgcac cggcgcgctg gtggcggggg ccgtgctgct ggcgaccctt
     8101 gtcgtgccgc tgccctaccg ggtgggcgcg gcggcgcgca tcgagggcgc cgagcagcgg
     8161 gtgctcgcgg cgccggtgga tggctatctg cgcaaggcct cggtacgccc cggcgacgcc
     8221 gtcaagaccg gtcaggtgct ggccgagatg gtggaccagg atctgatgct ggaagagcgc
     8281 aagtggcagg ccgagctgtc ccagcacgag aacgccttca tcgccgccat ggcgcgggcc
     8341 gaccgccccg gcttctccat cagccgcgcc aaggccggtg aggcccgcgc gcaactggag
     8401 ctggtgcgca gccagctggc acgcatgcag atcgtggcgc ccatcgacgg gctcgtcatc
     8461 gaaggcgatc tcacccaatc cctcggtgcg cccgtcaagc gcggtgacgc cctgctcacc
     8521 ctggcacccc agagccgcca tcggctgatg gtggaggtgg acgagcgcga catcgggcgg
     8581 gttgcgcccg gggcggacgg cgaactgaac ctggcggcgc tgcccggcga cagcctggca
     8641 ttccgcgtca tgcgcatcac cccggtggcg gtgcagcgta acggcaacaa cgtgttcgag
     8701 gtggaggccg cggtggacgg gggcgatgcc ctgcgtcccg gcatgcaggg cgtggcgagg
     8761 atcgatgcgg ggcaccggcc cctggtgtgg accctcaccc accgggcgtg ggactggctg
     8821 cgtctgaccc tcttcagctg ggggctgtga gatgacggcg tccctcttca gcccccactg
     8881 gtaccgggtg gcggacctgc ggccgcgtct gcgggcccag gtccggccga accgccagcg
     8941 ctggcgtgac cagctgtggt acgtgctggc cgatcaggcc accggccgcc accaccgcat
     9001 caacgagacg gcgtaccgct tcatagggcg ctgcgacggg cggcgcacgg tgcagcaggt
     9061 gtgggacgcg gtgctggacg cggacggcga cgcggcgccg acccaggacg aggtggtgga
     9121 gctcctcacc cggctgtacg aaagcgaact gctccagtcc gagcgcagtc ccgatgtgga
     9181 tgccctgttc cagcggcagt ccgaacgtgc ggccggtcag cgccgtgcca ccgtcaatcc
     9241 gctggcgttc cgtctggccc tgggcgaccc ccatcgctgg ctggtccggc tcgatccgct
     9301 ggcccatgcc gtgttccgcc ccggggtgtt cgggctgtgg ctggtgctga tgggcgttgc
     9361 cctgatcacg gccgggctcc attggccgga gctgcgcgac catgcggccc gccacgtgag
     9421 ttcgccgcgc tatctggcgc tgctgtggct gtgctatccg gtcatcaagg cgctgcacga
     9481 actgggtcac gccctggccg tgcggcgctg gggcggcgaa gtccacgaag ccggcgtcgt
     9541 gttgctggcc ctggtgcccg caccctacgt ggacgcctcc gcggcatcgg gcttcctggg
     9601 gcgcggaccc cgcgccatcg tcggggcggc cggcatcctg gtggagctgg cgctggcggc
     9661 gggcgggctg ttcctgtggc tcaacaccgc gccgggctgg atcaacgaca tcgccttcgt
     9721 ggtgatgacc gtgggcagcg tgtccacgct gctcatcaac ggcaacccgc tgctccgatt
     9781 cgatggctat cacgtactgt gcgatgccct cgatctgccc aatctggcgc cccgcagcac
     9841 cctctactgg agcgatctgc tgcgtcggcg cgtcctgcgc gtggcgggcg agccgcccca
     9901 gatgggcgcg ggcgaggcca agtggttgcg cctctacgcg cctctgtcgg cgctctaccg
     9961 cctggccgta ggcgtgtcca tcatgctctg gctggggtcg aagtggttcg tcctgggggt
    10021 ggtggcggcc gcctatctgg cggtggcgat gctgttccgg ccggtcgtgg gctgggtccg
    10081 ccagtccctc gccgcggccg cacccggccc ggccctggta cgggcgcaca gggggctgct
    10141 gctcctgctc ggcgtacccg gcctgctgct cttcgtcgtg ccgatgccgt atgtcagtgt
    10201 cgcgccggcg gtggtgtggc tgcctgaccg ggcccaggtg cgcccggacg tggacggctt
    10261 cgtgtcggaa ctgcccgtgc gcgacggcca gaacgtggcc cccggcgaac tgctggcggt
    10321 cctgcgcaat gacgatctgg ccgccgagcg ggagcgtctc gacagccgcc tgcagggcct
    10381 gcgctcggag cagttccggc tgctgctgcg cgaccccgtc ggcgcccaga accaggccga
    10441 ggagatcgcc cgcacggaag ccgagctgac ccgggccgac gagcgcctgg ccggcctgca
    10501 ggtgcgcgcc caggcgggcg gccggctggt catgccacgc caggccgatc tgcccggagc
    10561 cttcgtccgc aagggccagg tgctgggcta tgtctttccg gacggcgata ccctggtgcg
    10621 tgccgccgtg cccgagcggg acggacaccg ggtgcggctg gccggcagca cggcccgcgt
    10681 ccgcctggtc ggcggcggg
//
LOCUS       DAOQPA010000115        10631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3250_length_10631_cov_19.050681, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000115 DAOQPA010000000
VERSION     DAOQPA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_10631_cov_19.050681"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKX01_13900"
     CDS             <1..600
                     /locus_tag="PKX01_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ77270.1"
                     /translation="GLGADGHDFEPVVDEFDLDRLPPTRFVFPHAPMRAVTINGGYVM
                     RAWYDILSMDFSERREDPAGVLESAAQVEALIARENARGIADGHIVLAGFSQGGAIAL
                     HTALRHPRRLAGVLALSTYVPLADTLAAEAAPANRDVPLFMAHGLGDPVIPYAFSRRS
                     GDLLADLGYPLEWHDYPMDHALCLEEVRHIEDWLARVLA"
     gene            complement(619..1071)
                     /locus_tag="PKX01_13905"
     CDS             complement(619..1071)
                     /locus_tag="PKX01_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HMZ77271.1"
                     /translation="MDTRFLDDLNPGDRFTSPGITLTEAEIIDFALRYDPQSFHLDAV
                     AAAESPYGGLIASGFQSLALCFRLFIQSGILAESSMGSPGIDELRWLAPVRPGDTLHT
                     EVDVLEVRPSSTRPDRGIARFKYTAVNQRGETVLSFIVAHLLRRRPAG"
     gene            complement(1102..2001)
                     /locus_tag="PKX01_13910"
     CDS             complement(1102..2001)
                     /locus_tag="PKX01_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ77272.1"
                     /translation="MDLRALRYFIETVRHGSFTVAAERLCVTQSTVSKMLRQLEDEVG
                     QPLLHRDGRRIRVTDVGRILFDRGQEALGVMRELNREVADLTALARGELTVGLPPTVN
                     LFFTPVVKAFRERHPGVRLTLVERGGQIIEQQVASGELEVGVTLLPVDPALGLATRTV
                     GRHPIVAVGPADALWARQAQVDLSHLDGEALVLLHDDFALTRQLRDAFRRFGVEPRIV
                     AQSGQWDFLVALAAAGLGTALLPQPLLARMALHPELAVRPLADPALAWTVAHIWPPGR
                     YLSHAARAWLAVCEDVLDGGAIG"
     gene            2125..3639
                     /locus_tag="PKX01_13915"
     CDS             2125..3639
                     /locus_tag="PKX01_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HMZ77273.1"
                     /translation="MPLDRIRLPRLRERIMSPEEAASLFRDGMTVGMSGFTRAGDCKA
                     VPFALAERAAREPLQITLMTGASLGNDIDKVLSEANAIARRIPFQSDPSLRRKINAGE
                     VMFIDQHLSETVEQLRSGQIAPVDIAVVEASAITENGGIVPTTSVGNSASFAVLAKKI
                     IVEINLKMPVELEGLHDIYIPTYRPHRLPIPLIAPEQRIGLPYIPVDPEKIVAIVITE
                     KADSPSNVLPPDPETNAIAGHLNEFLYHEVKAGRLSPSLQPLQAGIGTIANAVLHGML
                     DSPFRDLKMYSEVLQDSTVELLDDGRLSFASASSITLSKATYERVLGQIERYKQRLVL
                     RPQEISNHPEVIRRLGLITINTALECDLYGNVNSTHVGGTHMMNGIGGSGDFARNAHL
                     SVFVTKSIAKDGAISSIVPMVSHTDHTEHDTDIIVTEHGLADLRGLAPRERARQIIDH
                     CTDPLYRDALRDYFLRACRRGGQTPHLLEEAFSWHLRYAREGAMLARDGRVEPA"
     gene            complement(3792..4841)
                     /gene="selD"
                     /locus_tag="PKX01_13920"
     CDS             complement(3792..4841)
                     /gene="selD"
                     /locus_tag="PKX01_13920"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HMZ77274.1"
                     /translation="MTDTPLRLTGLSHGGGCGCKIAPGVLTEMLARMPAAQPFANLLV
                     GTETADDAAVWRLNDTQALIATTDFFMPIVDDPFDFGRIAATNALSDVYAMGGTPILA
                     LAIVGMPLDKLPLDAIGRILAGGASVCADAGIPVAGGHSIDAPEPIYGLVALGLAHPD
                     VIKRNRDARAGDVLVLGKPLGVGILGSAMKKGLLDADGYAQMIATTTRLNTPGPVLAQ
                     LPGVHALTDVTGFGLLGHLLELCRGASLGATVEAARLPVMPAAVPLAQQGIGPGAIAR
                     NLASFGDAVRFDEDVADWQRHLVADAQTSGGLLVSCAAEAVPAVLEVFRQHGFAEAAV
                     IGAMHDGAAEVRVRR"
     gene            4873..5934
                     /gene="mnmH"
                     /locus_tag="PKX01_13925"
     CDS             4873..5934
                     /gene="mnmH"
                     /locus_tag="PKX01_13925"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HMZ77275.1"
                     /translation="MSVSPHAPLSVGLDRLAHFDAVIDVRSPSEFAEDHIPGALNCPV
                     LDDEERRRVGTLYKQVSPFEARKLGAALVSRNIARHLETHFLSKPKGWKPLVCCWRGG
                     QRSGAMTLVLNQVGWGARQLAGGYKAFRQQVMDDLVALPEGLSFRILCGPTGSGKTAL
                     LRRLAAQGGQVLDLEGLAVHKGSVLGGDVVRPQPGQKAFETGLWQALRGFDPARPVWV
                     EAESRRIGRLHLPPELFARLADGRCTLVRAPLEARVAYLLRDYADLIAEPAAFGARLE
                     RLIPLHGHERVLAWRAQIDAGDWETLARELIVTHYDPAYRRGGDGLYRHVAQAAVLDA
                     ASLDADALDALARGLANAI"
     gene            complement(5999..7408)
                     /locus_tag="PKX01_13930"
     CDS             complement(5999..7408)
                     /locus_tag="PKX01_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ77276.1"
                     /translation="MATRRTTKKAAITKLFVLDTNVLMHDPTSLFRFEEHDIYVPMMT
                     LEELDSNKKGMSEVARNARQASRSLDEIVNSSPDGIEAGISLEQASSQLASGRLYLQT
                     EAIDTRLPALPTAKGDNQILAVVMHLHEKFAKRPVILVSKDINMRIKARALGLAAQDY
                     FNDKVLEDTDLLYTGVKEIDAPFWERNGKDMESWKQEGRAYYRIKGPDTADLLINEFV
                     FQEGEQPLQAWVKDKAGRSVTFESLIDFSHQKNNVWGITARNREQNFALNLLMNPEID
                     FVTLLGQAGTGKTLLTLAAGLTQVLESKRYSEIIMTRVTVPVGEDIGFLPGTEEEKMA
                     PWMGALEDNLDVLNGSTGEGGEWGRAATRDLIRSRIKIKSLNFMRGRTFINKFLIIDE
                     AQNLTPKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWPHSGH
                     ITLQRGERSRLADHAAEVL"
     gene            complement(7433..7900)
                     /locus_tag="PKX01_13935"
     CDS             complement(7433..7900)
                     /locus_tag="PKX01_13935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMZ77277.1"
                     /translation="MLDQAAPDFSLPATGGQTLSLAQFRGRSVVLYFYPKDSTPGCTT
                     ESQQFRDLHDQFLAAGAVVLGLSRDSLKSHDNFRAKQALPFDLISDPDETACTLFGVM
                     KLKNMYGKQVRGIERSTFVIDAQGVLTREWRGVKVPGHAQEVLDYVKTLSTAA"
     gene            complement(7991..8368)
                     /locus_tag="PKX01_13940"
     CDS             complement(7991..8368)
                     /locus_tag="PKX01_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HMZ77278.1"
                     /translation="MKLHLDKAEQYNTISRYDATHVRVGAERYEGSVIVLPDRIEAGW
                     HMGGFDALDEAAFARLAGLGTEIVILGTGLRQRFPAPQLLRPLMQARIGFEVMDLGSA
                     CRTYNLLVAEHRAVAAALLFDPA"
     gene            8583..9809
                     /locus_tag="PKX01_13945"
     CDS             8583..9809
                     /locus_tag="PKX01_13945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ77279.1"
                     /translation="MQPILKSDKLANVCYDIRGPVLQKAKQMEDEGHKIIKLNIGNLA
                     AFGFDAPEEIQLDMIRNLPNAAGYSDSKGIFAARKAIMHYTQQKHIKGVTLEDIYVGN
                     GVSELIVMAMNALLNAGDEVLVPAPDYPLWTAAVSLSGGTPRHYLCDEANEWLPDLDD
                     IRAKITPRTRALVIINPNNPTGALYPEETLRGIVDIARQHGLILYADEVYDKVLYDGA
                     QHTSIASLSEDVLTITFNGLSKNYRSCGYRAGWLVVSGDKRAAADYIEGLNMLASMRL
                     CANVPGQYAIQTALGGYQSIDDLVAPGGRMRRQRDLAHELISAIPGVSCVKPKATLYM
                     FPRLDPAVYPIADDQQFIAQLLEQQRVLLVQGTGFNWPHPDHFRLVFLPHEDDLRDAI
                     GRIARFLDGYRKRSGT"
     gene            9877..>10631
                     /locus_tag="PKX01_13950"
     CDS             9877..>10631
                     /locus_tag="PKX01_13950"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMZ77280.1"
                     /translation="MKPIQVGLLGIGTVGGGTFTVLKRNEEEITRRAGRPIRITTVAD
                     KNLELARAVTGGDVKLTDDAFAVVSDPEIDIVVELIGGYGVAKELVLKAIENGKHVVT
                     ANKALLAVHGNEIFAAAQKKGVMVAFEAAVAGGIPIIKALREGLSANRIQWIAGIING
                     TTNFILSEMRDKGLPFADVLKEAQRLGYAEADPTFDIEGVDAAHKATLMASIAFGVPV
                     QFDKAYVEGISKLDATDIKYAEQLGYRIKLLGIA"
BASE COUNT         1673 a         3658 c         3618 g         1682 t
ORIGIN      
        1 ggcctgggcg ccgatgggca cgacttcgaa ccggtggtgg acgagttcga cctggaccgt
       61 ctgccgccga cccgcttcgt ctttccccac gcgccgatgc gcgccgtgac catcaacggc
      121 ggctatgtga tgcgcgcctg gtacgacatc ctgtccatgg atttttccga gcggcgcgag
      181 gatccggcag gtgtgctgga atccgccgcc caggtggagg ccctgatcgc ccgcgagaat
      241 gcccgcggga tcgccgatgg acacatcgtg ctggcgggct tctcccaggg cggcgcgatc
      301 gccctgcaca cggccctgcg gcacccgcgc cgtctggcgg gcgtgctcgc cctgtccacc
      361 tatgtgcccc tggccgatac cctcgccgcg gaagctgcgc cggccaaccg ggatgtgccc
      421 ctcttcatgg cccacggcct gggcgatccg gtgatcccct acgccttcag ccgccgctcg
      481 ggcgatctgc tggcggacct gggctatccg ctggaatggc acgactaccc catggaccac
      541 gccctgtgcc tggaggaagt gcggcacatc gaggactggc tcgcccgcgt cctcgcctga
      601 cacgacggta cgcgccgatc accccgccgg gcggcggcgc agcaggtggg cgacgatgaa
      661 gctgaggacg gtctcaccgc gctgattgac cgccgtgtac ttgaagcggg cgatgccccg
      721 gtccgggcgc gtggacgagg ggcgcacctc cagcacgtcc acctcggtgt gcagcgtgtc
      781 gcccgggcgc accggggcca gccagcgcag ctcgtcgata cccggcgagc ccatgctgga
      841 ctccgccagg atgccgctct ggatgaacag gcggaagcac agggcgagcg actggaagcc
      901 gctggcgatc aggccgccgt agggggattc ggccgcggcg acggcatcca gatggaagga
      961 ctgggggtcg tagcgcaggg cgaagtcgat gatctcggct tcggtgaggg tgatgcccgg
     1021 cgaggtgaag cggtcgccgg gattcaggtc gtccaggaag cgggtgtcca tggcagtctc
     1081 ctcgttgggg gtcggcgcat cttagccgat ggcgccgccg tcaaggacgt cctcgcacac
     1141 cgccagccag gcccgggcgg cgtgggacag gtagcggccg ggtggccaga tgtgggccac
     1201 cgtccacgcc aacgccggat cggccagcgg ccgcacggcc agctccggat gcagggccat
     1261 gcgggccagc aggggctggg gcaacagggc ggtgcccagg ccggcggcgg ccagggccac
     1321 caggaagtcc cactggccgc tctgggccac gatgcgcggc tccacgccga agcgacggaa
     1381 ggcgtcgcgc agctggcggg tgagggcaaa gtcgtcgtgg aggaggacca gcgcctcccc
     1441 gtccagatgg ctcaggtcca cctgcgcctg ccgggcccac agcgcatcgg caggccccac
     1501 cgcgacgatg ggatggcgcc cgacggtccg cgtcgccaga ccgagggccg ggtccaccgg
     1561 cagcagggtc acgcccacct ccagttcgcc gctcgccacc tgctgctcga tgatctgccc
     1621 gccccgttcc acgagggtca ggcgcacgcc cggatggcgt tcccgaaagg ccttgacgac
     1681 gggggtgaag aacagattca cagtgggggg cagacccacc gtgagttcgc cgcgggcgag
     1741 ggcggtgagg tcggcgacct cccggttcag ttcgcgcatc acgcccaggg cttcctgacc
     1801 gcggtcgaag aggatgcgcc ccacatcggt gacgcggatg cgccgcccat cccgatggag
     1861 caggggctgg ccgacttcgt cctccaactg gcggagcatc ttgctgacgg tggactgggt
     1921 gacgcagaga cgctcggccg ccacggtgaa gctgccgtgg cggacggtct cgatgaagta
     1981 gcgcaaggcc cgcaggtcca tctatgtatg aacgaaacga atgatgtgaa gtagtttaag
     2041 tcatccagtg catgtacgcg tctccctata ctcgtctccc aatgagggcc atccaatccg
     2101 gccccgccct ggagaggaga aaacatgccc ctggatcgca tccgtctgcc ccgcctgcgt
     2161 gaacgcatca tgtcccccga ggaggccgcg agcctgttcc gcgacggcat gaccgtgggc
     2221 atgagcggct tcacccgcgc cggcgactgc aaggccgtgc ccttcgcgct ggccgagcgg
     2281 gccgcccgcg agcccctgca gatcaccctg atgaccggcg cgtccctggg caacgatatc
     2341 gacaaggtgc tgtcggaagc caacgccatc gcgcgccgca tccccttcca gtccgacccc
     2401 agcctgcgcc gcaagatcaa tgcgggcgag gtaatgttca tcgaccagca cctctccgag
     2461 accgtcgagc agctgcgttc cggccagatc gcgccggtgg acatcgccgt ggtggaagcc
     2521 tcggccatca ccgagaacgg cggcatcgtg ccgaccacct cggtgggcaa ttcggcgagc
     2581 ttcgcggtgc tggccaagaa gatcatcgtc gagatcaatc tgaagatgcc ggtcgagctg
     2641 gaaggcctgc acgacatcta catcccgacc tatcgtccgc accgcctgcc gattccgctg
     2701 atcgcgccgg agcagcgcat cgggctgccc tacatcccgg tggatccgga gaagatcgtc
     2761 gccatcgtga tcaccgagaa ggccgacagc ccgtccaacg tgctgccgcc cgacccggag
     2821 accaacgcca tcgccggcca cctgaacgag ttcctctacc acgaggtcaa ggccgggcgc
     2881 ctgtcgccca gcctccagcc cctgcaggcg ggcatcggca ccatcgccaa cgcggtgctg
     2941 cacggcatgc tggattcgcc cttccgcgac ctcaagatgt actcggaagt gctccaggac
     3001 agcaccgtgg agctcctcga cgacggccgc ctgagttttg catcggcgtc ctccatcacc
     3061 ctgtcgaagg ccacctacga gcgcgtgctg ggccagatcg agcgttacaa gcagcgcctg
     3121 gtgctgcgtc cgcaggaaat cagcaaccat cccgaggtga tccgccgcct cggcctgatc
     3181 accatcaaca ccgcgctgga gtgcgacctc tacggcaacg tgaactccac ccatgtgggc
     3241 ggcacccaca tgatgaacgg catcggcggc tccggcgact tcgcccgcaa tgctcacctg
     3301 tcggtgttcg tgaccaagtc catcgccaag gacggggcca tctccagcat cgtgcccatg
     3361 gtgtcccaca cggaccacac cgagcacgac acggacatca tcgtcaccga gcacggtctg
     3421 gccgacctgc gcggtctggc cccgcgggag cgggcccgcc agatcatcga ccactgcacc
     3481 gatccgctgt atcgggatgc cctgcgggat tacttcctgc gggcctgccg acgcggcggc
     3541 cagaccccgc atctgctgga ggaggccttc tcctggcatc tgcgctatgc ccgcgagggc
     3601 gccatgctgg cgcgtgacgg tcgcgtcgag cccgcctgag aacccgctgg cgcgcgtgca
     3661 ggccgcgcgc ccggcgccgt cggcagcctt cggccggcgg cacgcttgac tgcctgcttc
     3721 tctcccccga ggccccccgc gttccgcgtc ccccgcgcgg aacgcggcgt gggtacgcga
     3781 tccgccgtgc ctcagcgtcg gacgcgcacc tccgcggccc cgtcgtgcat ggccccgatc
     3841 accgccgcct cggcgaagcc gtgctgccgg aacacctcca gcaccgccgg caccgcctcc
     3901 gccgcgcagc tcaccagcag gccaccgctg gtctgggcgt ccgccaccag gtggcgctgc
     3961 cagtccgcca cgtcctcgtc gaaacgcacc gcgtcgccga agctcgccag attgcgggcg
     4021 atggcgccgg ggccgatgcc ctgttgtgcc aggggcacgg cggccggcat caccggcaga
     4081 cgggcggcct ccacggtggc gcccaggctg gcgccgcggc acagctccag caggtggcca
     4141 agcaggccga agccggtcac gtcggtgagg gcatgcacgc cgggcagctg cgccagcacc
     4201 gggcccggcg tgttgaggcg ggtggtggtg gcgatcatct gggcgtagcc gtccgcatcc
     4261 agcaggccct tcttcatggc gctgccgagg atgcccacgc cgaggggctt gcccagcacc
     4321 agcacgtcgc cggcgcgggc gtcccggttg cgcttgatca cgtccgggtg ggcgaggccc
     4381 agggcgacca ggccgtagat cggctccggt gcgtcgatgc tgtgcccgcc agccaccgga
     4441 atgccggcgt cggcgcacac actggcgccg ccggcgagga tgcggccgat ggcgtccaga
     4501 ggcagcttgt cgaggggcat gccgacgatg gccagggcca ggatcggcgt gccgcccatg
     4561 gcgtagacgt ccgacagggc attggtggcc gcgatgcggc cgaaatcgaa ggggtcgtcc
     4621 acgatgggca tgaagaagtc cgtcgtggcg atcagcgcct gggtgtcgtt gagccgccac
     4681 acggccgcgt cgtcggcggt ctcggtgccc accagcaggt tggcgaaggg ctgggccgcg
     4741 ggcatgcggg ccagcatctc ggtgagcacg ccgggggcga ttttgcagcc gcatccgccg
     4801 ccgtgcgaca atccggtgag ccgcagaggt gtatccgtca tctcgttgtc gtcctgactt
     4861 tttccattac ccatgtccgt gtcgccccat gcgccgctat cggtcggtct cgaccggctg
     4921 gcgcacttcg acgccgtcat cgacgtgcgt tcgccttctg aattcgccga ggatcacatc
     4981 cccggcgccc tcaactgtcc ggtgctggac gacgaggagc gtcgccgcgt gggcaccctg
     5041 tacaagcagg tctcgccctt cgaggcgcgc aagctggggg ccgcgctggt gtcgcgcaac
     5101 atcgcgcggc atctcgagac gcattttctc agcaagccga agggctggaa acccttggtg
     5161 tgctgctggc gtggcgggca gcgcagcggc gccatgaccc tggtgctcaa ccaggtgggt
     5221 tggggcgcgc gccagctcgc cggtggctac aaggccttcc gccagcaggt gatggacgat
     5281 ctggtggcgc tgccggaggg gctgtccttc cgcatcctgt gcgggcccac cggcagcggc
     5341 aagaccgcgc tgttgcggcg actggcggcg cagggcgggc aggtcctgga cctggaaggc
     5401 ctggcggtgc acaaagggtc ggtgctgggg ggcgacgtgg tgcgcccgca gcccggccag
     5461 aaggccttcg agacgggcct gtggcaggcc ctgcggggct tcgatccggc ccgccccgtg
     5521 tgggtggagg cggagagccg gcgcatcggg cgcctgcacc tgccgccgga actcttcgcg
     5581 cgtctggccg atggtcgctg caccctcgtg cgtgcgccgc tggaggcgcg ggtggcctac
     5641 ctgttgcgcg actacgccga tctgatcgcc gagcctgccg ccttcggcgc ccgcctggag
     5701 cgcctgatcc cgctgcatgg acacgagcgg gtgctggcct ggcgggcgca gatcgacgct
     5761 ggcgactggg agaccctcgc ccgcgagctg atcgtgaccc actacgatcc ggcctaccgg
     5821 cgcggcgggg acggcctcta ccggcacgtg gcgcaggcgg ccgtgctcga cgcggcgtcg
     5881 ctggatgccg atgcgctgga cgccctcgcg cgtggcctgg ccaacgccat ctgaccgacc
     5941 gtgaacgcac gaccggccgc gcagggcggc cggccgacgt gacggcaagg agaacgtcct
     6001 acagtacttc cgccgcgtga tccgccaggc gcgagcgttc gccccgttgc agggtgatgt
     6061 ggcccgagtg gggccagccc ttgaagcggt ccaccacgta ggtcaggccc gacgaaccct
     6121 cggtgaggta gggcgtgtcg atctgggcga tgttacccag gcataccacc ttggtgccgg
     6181 ggccggcgcg ggtgatgagg gtcttcatct gcttgggcgt gaggttctgg gcctcgtcga
     6241 tgatgagaaa cttgttgatg aaggtgcgtc cgcgcatgaa gttgagggac ttgatcttga
     6301 tgcggctgcg gatcaggtcg cgggtggcgg cgcggcccca ttcgccgcct tcgccggtgg
     6361 agccgttgag cacgtccagg ttgtcctcca gcgcgcccat ccacggcgcc atcttctcct
     6421 cctcggtacc cggcaggaag ccgatgtcct cgcccaccgg cacggtgacg cgggtcatga
     6481 tgatctcgct gtagcgcttc gactccagca cctgggtgag cccggcggcg agcgtcagca
     6541 gggtcttgcc ggtgccggcc tggccgagca gggtgacgaa gtcgatctcc gggttcatga
     6601 gcaggttgag ggcgaagttc tgctcccggt tgcgggcggt gatgccccac acgttgttct
     6661 tctggtggga aaagtcgatg agggattcaa aggtgacgct gcgcccggcc ttgtccttga
     6721 cccaggcctg gagcggctgc tcgccttcct ggaagacgaa ttcattgatc agcaggtcgg
     6781 cggtgtccgg acccttgatg cggtagtagg cccggccttc ctgtttccag gactccatgt
     6841 ccttgccgtt gcgctcccag aacggggcgt cgatctcctt gacgccggta tacaggaggt
     6901 cggtgtcctc cagcaccttg tcgttgaagt agtcctgcgc cgccaggccc agcgcgcggg
     6961 ccttgatgcg catgttgatg tccttcgaga cgaggatcac cgggcgcttg gcgaattttt
     7021 cgtggaggtg catcaccacc gccaggatct ggttgtcgcc cttggcggtg ggcagcgcgg
     7081 ggaggcgggt gtcgatggcc tcggtctgca ggtacagacg gccgctggca agctggctgg
     7141 aggcctgttc cagggagatg ccggcctcga tgccgtcggg gctggagttg acgatctcgt
     7201 caaggctgcg gctggcctgg cgggcgttgc gggcgacttc cgacatgccc ttcttgttgg
     7261 agtccagctc ctccagggtc atcatcggca cgtagatgtc gtgctcctcg aagcggaaga
     7321 ggctggtggg gtcgtgcatc agcacgttgg tgtcgagaac gaacagcttg gtgatggcgg
     7381 ctttcttggt cgttcggcgg gtggccatgg tgggcggagc ctcgggctgg ggttaggcgg
     7441 cggtggagag cgtcttcaca tagtcgagca cttcctgggc gtggccaggc accttcacgc
     7501 cgcgccattc acgcgtcagg acgccctggg cgtcgatcac gaaggtgctg cgttcgatgc
     7561 cccgcacctg tttgccgtac atgtttttca gtttcatgac gccgaacagg gtacaggcgg
     7621 tctcgtccgg atcggagatc aggtcgaagg gcagggcctg tttggcgcgg aagttgtcgt
     7681 gggacttgag gctgtcgcgg gacaggccca gcacgaccgc gccggccgcc agaaactggt
     7741 cgtgcaggtc gcggaactgc tggctttcgg tggtgcagcc gggggtgctg tccttggggt
     7801 agaagtacag gacgacgctg cggccccgga actgggcgag gctgagggtc tggccgccgg
     7861 tggcgggcag gctgaaatcg ggtgcggcct ggtcgagcat gctgactccg gaagtggggt
     7921 gaggtccggt ggcagcgtaa tcccaaggcg ccggccttgg caatcggccc ggaggccggc
     7981 gcggcgcgcc tcaggccgga tcgaagagca gggcggccgc cacggcccgg tgttcggcga
     8041 cgagcaggtt gtaggtgcgg caggcggagc ccaggtccat cacctcgaag ccgatgcgcg
     8101 cctgcatcag cggacgcagc aactgcggcg ccgggaagcg ctgacgcagg ccggtgccga
     8161 ggatgacgat ctcggtaccg agcccggcga ggcgggcgaa ggcggcctcg tcgagggcat
     8221 cgaagccacc catgtgccag ccggcttcga tacggtcggg caggacgatc acgctgcctt
     8281 cgtagcgttc ggcgccgacc cgcacgtggg tcgcgtcgta acgggagatg gtgttgtact
     8341 gctcggcttt gtccagatgc agtttcatcg cgggggagtc tctccggggg cggtcggtgg
     8401 ggcgggctga tttgcccggt caggggcgct tgggtaacat tataactttg cgtcgccgcg
     8461 tacctgccgg gtccgtcccg tcgccgcggg ggcccgcctc ccccagcatg agccgccacg
     8521 tccgcccgcg cgtaccgtcc cgcgccgccc cgaagagagt ttcgcccagc aaggaaagca
     8581 ccgtgcaacc catcctcaag tccgacaaac tcgccaacgt ctgctacgac atccgcggcc
     8641 ccgtgctgca gaaggccaag cagatggagg acgaagggca caagatcatc aagctgaaca
     8701 tcggcaacct cgccgccttc gggttcgacg ccccggagga gatccagctc gacatgatcc
     8761 gcaatctgcc caacgcggca ggctactcgg actccaaggg catcttcgcc gcccgcaagg
     8821 cgatcatgca ctacacccag cagaagcaca tcaagggcgt gacgctggaa gacatctacg
     8881 tgggcaacgg cgtgtccgag ctcatcgtga tggccatgaa cgcgctcctc aacgctggtg
     8941 acgaggtgct ggtgccggcg cccgactatc ccctgtggac cgccgccgtg agcctctccg
     9001 gcggcacccc gcgccattac ctgtgcgacg aggccaacga gtggctgccc gatctggacg
     9061 acatccgcgc caagatcacg ccgcgcaccc gcgccctcgt catcatcaac cccaacaacc
     9121 cgaccggcgc cctgtacccc gaagagaccc tgcgcggcat cgtggacatc gcccgccagc
     9181 acggcctgat cctctacgcc gacgaggtct acgacaaggt gctgtacgac ggcgcccagc
     9241 acacctccat cgcctccctg tcggaggacg tgctcaccat caccttcaac ggcctgtcca
     9301 agaactaccg ctcctgcggc tatcgcgccg gctggctggt ggtgtccggt gacaagcgcg
     9361 ccgccgccga ctacatcgaa ggcctcaaca tgctggcctc catgcgcctg tgcgccaacg
     9421 tgcccggcca gtatgcgatc cagaccgccc tcggcggcta ccagagcatc gacgacctgg
     9481 tggcgcccgg cggccgcatg cgccgtcagc gcgacctggc ccacgagctg atcagcgcca
     9541 ttcccggcgt cagctgcgtc aagcccaagg ccaccctgta catgttcccg cgcctcgacc
     9601 cggcggtgta ccccatcgcc gacgaccagc agttcatcgc ccagctgctg gagcagcagc
     9661 gcgtgctgct ggtccagggc accggcttca actggcccca cccggaccac ttccggctgg
     9721 tcttcctgcc ccacgaggac gacctgcgcg acgccatcgg gcgcatcgcc cgcttcctcg
     9781 acggctaccg caagcgttcc ggcacctgat ccccgacggg gcgccggttg tggcgccccg
     9841 cgttcatcca cgttcactca cacgacccac tcgaccatga aacccatcca agttggcctc
     9901 ctgggcatcg gcaccgtcgg cggtggcacc ttcaccgtcc tcaagcgcaa cgaagaagaa
     9961 atcacccgcc gcgccggccg gccgatccgg atcaccaccg tggccgacaa gaacctggag
    10021 ctggcccgcg ccgtcaccgg cggcgacgtc aagctcaccg acgacgcctt cgccgtggtc
    10081 agcgaccccg aaatcgacat cgtcgtcgaa ctgatcggtg gctacggcgt ggccaaggag
    10141 ctggtgctca aggccatcga gaacggcaag cacgtggtca ccgccaacaa ggcgctgctc
    10201 gccgtgcacg gcaacgagat cttcgccgcc gcccagaaga agggcgtgat ggtggccttc
    10261 gaggccgccg tggccggggg catccccatc atcaaggccc tgcgcgaagg cctctccgcc
    10321 aaccgcatcc agtggatcgc cggcatcatc aacggcacca ccaacttcat cctctccgag
    10381 atgcgcgaca agggcctgcc cttcgccgac gtgctcaagg aagcccagcg cctgggctac
    10441 gccgaagccg accccacctt cgacatcgaa ggcgtggacg ccgctcacaa ggcgaccctg
    10501 atggcctcca tcgcctttgg cgtgccggtg cagttcgaca aggcctacgt ggaaggcatc
    10561 agcaagctcg acgccaccga catcaagtac gccgagcagc tgggctaccg catcaagctg
    10621 ctgggcatcg c
//
LOCUS       DAOQPA010000116        10496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3310_length_10496_cov_19.933627, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000116 DAOQPA010000000
VERSION     DAOQPA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_10496_cov_19.933627"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /gene="dnaK"
                     /locus_tag="PKX01_13955"
     CDS             <1..1502
                     /gene="dnaK"
                     /locus_tag="PKX01_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMZ77281.1"
                     /translation="NDSQRQATKDAGRIAGLEVKRIINEPTAAALAFGMDKKPGDSKI
                     AVYDLGGGTFDISIIEIADIDGEHQFEVLATNGDTFLGGEDFDQRIIDYIVTEFKKEQ
                     GVDLKNDVLALQRLKEAAEKAKIELSSGQQTEINLPYITADATGPKHLALKITRAKFE
                     SLVEDLVERTIEPCRVALKDAGVKVSDIDDVILVGGQTRMPKVIEKVKEFFGKDPRRD
                     VNPDEAVAVGASIQGGVLQGEVKDVLLLDVTPLSLGIETLGGVMTKLIQKNTTIPTKA
                     SQVFSTADDNQSAVTIHVLQGEREMATGNKSLGQFNLSDIPPAPRGMPQIEVTFDIDA
                     NGILHVSAKDKATGKENKITIKANSGLSDDEIQRMVKDAEAHAEEDKKAHELVDARNQ
                     LDTLVHSVKKALTEHGDKIDGDEKGKIEAAIKEAEDAIKSGDKAAIDAKSEALATVSQ
                     KLGEKVYAQAQAEAAAGGAGAGAGAGSSGAKASDADVVDAEFTEVKDKK"
     gene            1597..2724
                     /gene="dnaJ"
                     /locus_tag="PKX01_13960"
     CDS             1597..2724
                     /gene="dnaJ"
                     /locus_tag="PKX01_13960"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMZ77282.1"
                     /translation="MAKRDLYEVLGVNRDASDDELKKAYRKLAMKYHPDRNPDNKDAE
                     EKFKEAKEAYEILTDANKRAAYDRYGHAGVDPSMGGGGGGGQGFEGFADAFGDIFGDI
                     FGGGGGRGRSNVYRGADLRYNLEISLEEAARGADKTIRIPAVEECDSCHGSGAKPGTQ
                     PKTCPTCGGAGQVRIQQGFFSIQQTCPNCHGSGRIIPDPCKACGGAGRVKRQKTLEVK
                     IPAGIDSGMRLRHGGHGEPGVNGGPAGDLYVEIHIKAHPVFQRDGDDLHCEMPISFAT
                     AALGGDIEIPTLDGAASIRIPPETQSAKVFRLRGKGIRNVRSHAPGDLMVHVIVETPV
                     KLTDRQKELLREFDDIASRNSERHNPKATSWMDKMKNFFSG"
     gene            2886..4043
                     /locus_tag="PKX01_13965"
     CDS             2886..4043
                     /locus_tag="PKX01_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77283.1"
                     /translation="MKLIHAIKSAAASLGALAVLSLSTPLCNADPGVTDSTITLGMSA
                     PFSGPNGAYGAEMKEGALAYFAQLNAAGGIGGRKVDLVALDDGYDTERAVSNTRKLIN
                     ESNAFALMAFYGSSPTTESMKVFAEAKVPLIGTISGASTLRTPVNRYMFHLRASYADE
                     TETLVNHLVGLGITHIAVFYQNDGFGKSGLEGVTAALGRHKLKPVAIGSVERNSIQVE
                     NAVKDIAKVSPQAVIMVTLYKPTAEFIRQMRKAGQMPQFSTLSPIGADLIVNELGAQQ
                     AHGLGISQVMPYPWNDTVPVVRDYQRALQAFAKHKNYSYYGLEGYLNAKLVAEALRRA
                     GKEPTREKLIAALEGGPFELGGYKVAYSSSNHNGSRFVDLTVLSRDGRVLR"
     gene            complement(4119..5501)
                     /gene="cysS"
                     /locus_tag="PKX01_13970"
     CDS             complement(4119..5501)
                     /gene="cysS"
                     /locus_tag="PKX01_13970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMZ77284.1"
                     /translation="MLKIHNSLSRKKEQFCPIEPGKVRMYVCGMTVYDFCHLGHARVM
                     VVFDMVARWLRASGYAVTYVRNITDIDDKIIKRAQENGESIRSLTDRFIDAMHEDADA
                     LGVLRPDHEPRATEYVPAMQTLIGSLVEHGLAYKAANEDMCYAVRKFDGYGKLSGKSL
                     DELRAGERVEVAGGKQDPLDFVLWKHARADEPEEVKWASPWGAGRPGWHIECSAMSSE
                     LLGAQFDIHGGGADLQFPHHENEIAQSEGAHQCTFVNYWMHNGFVRVDDEKMSKSLGN
                     FFTIRDVLKAYAPEVVRFFILRAHYRSPLNYSDAHLVDARHALSRLYTALRNVPADGT
                     VVNWDHPLAVRFKAALDDDFNTAEAIAVLFDLANEVNRERAPAMAALFKGLANILGLL
                     EQDPAAFLQAGTEAGGEDEGAAIQARIEARLAAKKARNFAEADRIRAELLADGIVLED
                     TPLGTIWRRA"
     gene            5744..6874
                     /locus_tag="PKX01_13975"
     CDS             5744..6874
                     /locus_tag="PKX01_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ77285.1"
                     /translation="MSKRALAASLLLACLAASTPVLADAPQDIQALIKQGQFTQALER
                     TDQALAGKPRDAQLRFLKGVVLTELNRGNDAIAIFQKLTEDYPELPEPYNNLAVLYAQ
                     QRQYDKARNALEMAIRTHPSYATAHENLGDVYARLASQAYDKALALDSSNTSAQNKLA
                     LIREMISPTGKNVRPTAPAPVSVAAAPAAPAVTPPPVAKPAPAPIPATPAAPAPTTVA
                     QAAPKAPPVVASPPVPPAPPPAAKPTQKDDPKPAAKPEPRDESAAQSAAITRSVQGWA
                     TAWSHKDIKGYLSYYARDFDPPKGLSRKAWEEERGQRLTKPGSIEVALENVQIASVAG
                     DKATVRLRQHYKSANLSTSSNKTLILVRQDGKWLIQQERVGG"
     gene            6979..8091
                     /locus_tag="PKX01_13980"
     CDS             6979..8091
                     /locus_tag="PKX01_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMZ77286.1"
                     /translation="MVAAPGGPDAALAEALQDVSQGRLTTALARIDRLIAAYPTFRLA
                     QLIRGDLLLAQAGPISGFGNVAEAPPDRLADLRDEAVVRLKAFRNRPPANYVPRYLLQ
                     MEPEQKYAVVVDTQSARLYLYQNDAGRPRYVADYYISHGKLGAEKFREGDKRTPIGVY
                     YVTSSLPRQKLNDFYGSGAFPISYPNEWDRQQGRDGHGIWLHGTPSDTYSRPPRASDG
                     CVVLTNPDLEALGGYLQIGITPVIISHNIEWLSLEDWAGERTALGKAVEAWRSDWESR
                     DVKRYLSHYSSRFRNNEGGYDEWSRQKQLVAAGKSWIKVSLNHLSIFRNPGKQDMAVV
                     SFEQDYRSNNLSNTLRKRQYWMKEGGRWKIVYEGFA"
     gene            8130..8699
                     /locus_tag="PKX01_13985"
     CDS             8130..8699
                     /locus_tag="PKX01_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ77287.1"
                     /translation="MKCALAALILASQALVASAAPQVELKTTAGTIVVDLNSEKAPKT
                     VANFVDYVKAGHYNGVIFHRVIDGFMIQGGGFTPEMKEKPTRAPIQSESKNGLKNDAG
                     TLAMARTQDPHSASAQFFINLVDNAPLNYPSPDGWGYTVFGKVVTGMDVVRKIGQTPT
                     GNRGFYRDVPLEAVVIQSARVLPDAPAAK"
     gene            8732..9226
                     /locus_tag="PKX01_13990"
     CDS             8732..9226
                     /locus_tag="PKX01_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ77288.1"
                     /translation="MAVKLVTNFGDITLELDADKAPVTVKNFLGYVEAGHYNNTVFHR
                     VINGFMIQGGGFEPGMKQKDTGEQIQNEANNGLKNDAGTIAMARTQAPHSATAQFFIN
                     VADNDFLNHRSPDIQGWGYCVFGRVTEGMDVVNKIKGVKTGSKGFHQDVPVEDVIIER
                     AEIL"
     gene            9255..9980
                     /locus_tag="PKX01_13995"
     CDS             9255..9980
                     /locus_tag="PKX01_13995"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMZ77289.1"
                     /translation="MRVHFISDLHLSADRPALIGLFERYLAGPARTAEQLYILGDLLE
                     YWCGDDDTDDPLARRLCGALKDLSASGCQVSFMAGNRDLLIGPAFAERAGFTLLPEPT
                     LIELDGQPTLLCHGDSLCTDDVAYQVYRTQVRDPVWQQGFLAQPLAARKQFIQGLRMK
                     SEAAKSGKAADIMDVNADAVAGLLRAHAYPRLIHGHTHRPATHHLTVDGHACERWVLA
                     DWHDDQGEVLIWDGDRLLRQILR"
     gene            complement(10050..>10496)
                     /locus_tag="PKX01_14000"
     CDS             complement(10050..>10496)
                     /locus_tag="PKX01_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMZ77290.1"
                     /translation="FNAWVILKGLETLSIRMAAQSAAALELARWLEAQPQVARVHYPG
                     LPSHPQHELALRQQKSGGAIVSFEVQGGRDEAWRVVDHTQVMSITANLGDTKTTITHP
                     ATTTHGRISPEAREAAGIRESLLRVAVGLESVEDLKADLARGLALL"
BASE COUNT         2071 a         3703 c         3091 g         1631 t
ORIGIN      
        1 tcaacgacag ccagcgtcag gccaccaagg acgccggccg catcgccggc ctggaagtca
       61 agcgcatcat caacgaaccc accgccgcgg cgctggcctt cggcatggac aagaagcccg
      121 gcgactccaa gatcgccgtg tatgacctgg gcggcggcac cttcgacatc tccatcatcg
      181 agatcgccga catcgacggc gagcaccagt ttgaagtgct ggccaccaac ggcgacacct
      241 tcctgggcgg cgaggacttc gaccagcgca tcatcgacta catcgtcacc gagttcaaaa
      301 aggaacaggg cgtcgatctc aagaacgacg tgctggccct gcaacgcctg aaggaagccg
      361 ccgaaaaggc caagatcgag ctgtcctccg gccagcagac cgaaatcaac ctgccctaca
      421 tcaccgctga cgccaccggc ccgaagcacc tggcgctgaa aatcacccgc gccaagttcg
      481 aatcgctggt ggaagacctg gtggagcgca ccatcgagcc ttgccgtgtc gccctcaagg
      541 acgccggcgt caaggtgtcc gacatcgacg atgtgatcct ggtgggcggc cagacccgca
      601 tgcccaaggt catcgagaag gtgaaggaat tcttcggcaa ggatccgcgc cgtgacgtga
      661 acccggacga agccgtcgcc gtcggcgcct ccatccaggg cggcgtgctg cagggcgaag
      721 tgaaggacgt gctgctgctg gacgtcaccc ccctgtccct gggcatcgaa accctgggcg
      781 gcgtgatgac caagctgatc cagaagaaca ccacgatccc gaccaaggcg tcccaggtgt
      841 tctccaccgc tgacgacaac cagtccgccg tgaccatcca cgtgctgcag ggcgagcgcg
      901 aaatggccac cggcaacaag agtctgggcc agttcaacct gtccgacatc ccgccggccc
      961 cgcgcggcat gccgcagatc gaggtgacct tcgacatcga cgccaacggc atcctgcacg
     1021 tgtccgccaa ggacaaggcc accggcaagg aaaacaagat caccatcaag gccaactcgg
     1081 gcctgtcgga tgacgagatc cagcgcatgg taaaggacgc cgaggcccac gccgaagagg
     1141 acaagaaggc ccacgaactg gtggatgcgc gtaaccagct cgacaccctg gtgcactccg
     1201 tcaagaaggc cctgaccgag cacggcgaca agatcgacgg cgacgagaag ggcaagatcg
     1261 aagccgccat caaggaggcc gaggacgcca tcaagtccgg cgacaaggcc gctatcgacg
     1321 ccaagagcga agccctcgcc actgtgagcc agaagcttgg tgaaaaggtt tatgcccagg
     1381 cccaggccga agccgccgcc ggtggcgcag gggccggcgc cggagccggc agcagcggcg
     1441 ccaaggcgag tgacgccgat gtggtggatg ctgaattcac cgaagtgaaa gacaagaagt
     1501 aatcgccccc gggtctccgg acccccacgg ccgagcccga accggagcag tccgggccgg
     1561 ctcggccttt ccacacgata gccagagcag gcaaccatgg cgaaacggga tttatacgaa
     1621 gtcctcggcg tcaatcgcga cgcatcggac gacgaactga agaaggccta ccgcaagctg
     1681 gccatgaaat accatccgga ccgcaatccg gacaacaagg acgccgaaga gaaattcaag
     1741 gaggccaagg aggcctacga gatcctcacc gacgccaaca agcgcgccgc ttacgaccgc
     1801 tacggccatg ccggcgtcga tcccagcatg ggcggcggcg ggggtggcgg tcagggcttc
     1861 gagggcttcg ccgatgcctt cggcgacatc ttcggcgaca tctttggcgg cggcggcgga
     1921 cggggacgtt ccaacgtcta ccgcggtgcc gacctgcgct acaacctgga gatctctctg
     1981 gaggaagccg cccgcggcgc cgacaagacc atccgcatcc cggccgtcga agaatgcgac
     2041 agctgtcacg gcagcggcgc caaacccggc acccagccca agacctgccc cacctgcggc
     2101 ggcgccggtc aggtgcgcat ccagcagggc ttcttctcca tccagcagac ctgccccaac
     2161 tgccacggca gcggccggat cattcccgat ccgtgcaagg cctgcggtgg cgcgggtcgc
     2221 gtcaagcgcc agaagaccct ggaggtgaag atcccggccg gcatcgacag tggcatgcgc
     2281 ctgcgccacg gcggacacgg cgagcccggc gtcaatggtg gcccggcggg cgacctttac
     2341 gtggagatcc acatcaaggc acacccggtc ttccagcgcg acggcgacga cctgcactgc
     2401 gagatgccca tcagctttgc cacggcggcc ctcggcggcg acatcgagat tcccaccctc
     2461 gacggcgcgg cgagcatccg cattccgccg gaaacccaga gcgccaaggt cttccgcctg
     2521 cgcggcaagg gcatccgcaa tgtgcgctcc cacgcccctg gcgacctgat ggtgcatgtc
     2581 atcgtggaaa cgccggtcaa actgaccgac cgacagaagg aactgctgcg ggaattcgac
     2641 gacatcgcct cgcgcaattc cgagcgccac aaccctaaag ccacgtcctg gatggacaag
     2701 atgaagaact tcttcagcgg ctgatcgacg tccccgtgcc gctcatcgac acaggccgga
     2761 ccctcgcggg tgccggcctg tgtctttgtg ccgatcgaac cgggggcacc gatgtgtctt
     2821 ttccgtaaca ctttcgtttt ggatatactc gtcgtcgccc tcccataacg acaacaggca
     2881 catccatgaa gctgatccac gccatcaagt cagccgccgc gtccctgggc gccctcgccg
     2941 tcctgagtct ttccacaccg ctctgcaacg ccgaccccgg ggttaccgac agcaccatca
     3001 ccctcggcat gtccgccccc ttttcaggcc cgaacggtgc ctacggggcc gagatgaagg
     3061 aaggcgcgct ggcctacttc gcccaactca atgcagccgg tggcatcggc gggcgcaagg
     3121 ttgaccttgt cgccctggac gacggctacg acaccgaacg cgcggtgtcc aacacccgca
     3181 agctcatcaa cgaaagcaac gccttcgcgc tgatggcctt ctacggatcc tcgcccacca
     3241 ccgagtcgat gaaggtgttt gccgaggcca aagtgcccct catcggcacc atcagtggcg
     3301 cctccacgct gcgcacgccg gtcaaccgct acatgttcca cctgcgcgcc agctatgccg
     3361 acgaaaccga gaccctggtc aaccatctgg tcggactggg catcacccac attgccgtgt
     3421 tttaccagaa cgacggattc ggcaagtccg ggctcgaagg cgtcaccgca gcactcggac
     3481 gccacaagct caagccggtc gccatcggca gcgttgaacg gaattcaatc caggtggaaa
     3541 acgcggtcaa ggacattgca aaggtctccc cgcaggccgt aatcatggtc accctgtaca
     3601 agccgactgc cgaattcatc cgtcagatgc gcaaggcggg ccagatgccc cagttctcca
     3661 ctctgtcgcc catcggtgcc gacctcatcg tgaatgaact gggcgcccag caggcccacg
     3721 gtctgggcat ctcccaggtc atgccctacc catggaacga caccgtgccc gtggtgaggg
     3781 attatcaacg ggcccttcag gctttcgcca agcacaagaa ctacagctat tacgggcttg
     3841 aaggctacct caacgccaag ctcgtggcgg aagccctacg ccgcgccggc aaggaaccga
     3901 cccgtgaaaa gctgatcgcg gcgcttgaag gcggcccctt cgaactcggc ggctacaagg
     3961 tggcctattc gtccagcaac cacaacggtt cccgcttcgt cgacctgacc gtcctgtccc
     4021 gcgacggccg cgtgctgcgc tgaacgcccc gttcggaccc catctgtgac aagggctccc
     4081 cgcagggagc cctttttcgt tgcgcgacgc ggggtacgtc aggcccgacg ccagatggta
     4141 cccaagggcg tatcttcgag cacgataccg tcagccagca gttccgcccg gatgcggtcc
     4201 gcctctgcaa aattgcgcgc cttcttggcg gccagacggg cctcgatgcg cgcctggatg
     4261 gcggcgccct catcttcgcc cccggcctcc gtcccggcct gcaggaaggc agccggatcc
     4321 tgctccagca gcccgaggat gttcgccagc cccttgaaca aggccgccat ggctggcgcg
     4381 cgctcccggt tcacctcgtt ggccagatcg aacagcaccg caattgcttc ggccgtgttg
     4441 aaatcgtcgt ccagagccgc cttgaagcgc accgccaggg gatggtccca gttcaccacc
     4501 gtcccgtcag ccggcacgtt gcgcaatgcc gtatacagac gcgacagagc gtggcgggca
     4561 tccaccagat gggcatcgga atagttcagc gggctgcgat agtgcgcacg caggatgaag
     4621 aaacgcacga cttccggtgc gtaggccttg agcacgtcac ggatggtgaa gaagttgccg
     4681 agactcttcg acattttctc gtcgtccacc cgcacgaacc cattgtgcat ccagtaattc
     4741 acgaaggtac actggtgggc gccctcgctc tgggcaatct cgttctcatg gtgcggaaac
     4801 tgcagatcgg ccccgccgcc atggatgtcg aactgcgcgc caaggagttc cgagctcatc
     4861 gcggagcact cgatgtgcca gcccggtcgg ccggcgcccc agggcgaggc ccacttgacc
     4921 tcctccggtt cgtccgcacg ggcgtgcttc cacaacacga aatccagcgg atcctgcttg
     4981 ccgccggcca cctccacccg ctcaccggcg cgcagttcgt cgagggactt gcccgagagc
     5041 ttgccgtagc cgtcgaactt gcgcaccgcg taacacatgt cttcattggc cgccttgtag
     5101 gccagcccgt gctcgaccag actgccgatc agcgtctgca tggccggcac gtactccgtc
     5161 gcacgcggtt cgtgatcggg tcgcaggaca cccagagcat ccgcatcctc atgcatcgca
     5221 tcgatgaagc gatcggtcaa ggaacggatg ctttcgccgt tctcctgggc acgcttgatg
     5281 atcttgtcgt cgatgtcggt gatgttgcgc acgtaggtca cggcataacc gctggcccgc
     5341 agccagcgcg ccaccatgtc gaacaccacc atcacgcggg catgccccag gtgacagaag
     5401 tcgtagaccg tcatcccgca cacatacatc cgcaccttgc ccggctcgat ggggcagaac
     5461 tgctcctttt ttcgcgaaag ggagttatga attttcagca tgaaggaaag gaaaaccgca
     5521 aatgggggaa atctctggaa tgccggcacg cgccgtcacc agatcgcatg ccgcctcacg
     5581 cgaccggagt cggcgcgggg gtcttctcga ctggggacgc acgggggaat gctcatggct
     5641 ggccggcaag cttcttggta gaatgcgcgg ccatgatgcc ggtgcgccgg cgacgcaatc
     5701 ttcccagaac tctccgggcc tgaaggccaa ctcagtatac cacatgtcga aacgtgccct
     5761 ggccgcatcc ctcctgctgg cctgccttgc cgccagcact ccggtcctgg cggacgcccc
     5821 tcaggacatc caggcgctga tcaagcaggg ccagttcacc caggcgctcg aacgaaccga
     5881 ccaggcgctc gccggcaagc cccgcgacgc ccagttgcgc ttcctcaagg gtgtcgtgct
     5941 caccgaactg aaccggggca acgacgccat cgcgatcttc cagaagctga ccgaagacta
     6001 cccggagctg ccagagccct acaacaacct cgcggtgctc tacgcccagc agcgccagta
     6061 cgacaaggcc cgcaatgccc tggagatggc catccgcacc catcccagct acgccacggc
     6121 ccacgaaaat ctcggtgacg tgtatgcgcg cctggccagc caggcttacg acaaggccct
     6181 ggcgctggac tcctccaaca catccgccca gaacaagctg gccctgatcc gcgagatgat
     6241 cagtccgacc ggcaagaacg tccgtccgac ggcccctgcg ccggtcagcg tggccgcagc
     6301 gcctgcggcc cccgccgtga cgccgcctcc ggtcgccaaa ccggctcctg ccccgatacc
     6361 ggcgacacct gccgccccgg cgccgaccac cgtcgcccag gcagccccca aggcgccgcc
     6421 cgtcgtcgcc tcgccccctg tcccgccggc accgcccccc gcggccaagc cgacacagaa
     6481 ggacgatccc aagcctgctg ccaaacctga accccgcgac gaaagcgctg cccagtcggc
     6541 cgccatcacc cgttcggtcc agggctgggc gaccgcctgg tcccacaagg acatcaaggg
     6601 ctacctgtcc tattacgccc gtgacttcga tccacccaag gggctcagcc gcaaggcctg
     6661 ggaagaggaa cgcggccagc gcctgaccaa gccgggcagc atcgaggtgg ccctcgagaa
     6721 cgtccagatc gccagcgtcg cgggtgacaa ggccaccgtg cggctgcgtc agcattacaa
     6781 gtctgccaac ctgtccacct cgtccaacaa gacgctcatc ctcgtccgtc aggacggcaa
     6841 gtggctgatc cagcaggagc gggtcggcgg ttgatgcggt cggtgatggc ggtctttcgc
     6901 tgggcgtgcg ccgccgtgct ggcgtgcggc gcacttgcgg ccctttcggg accgccgcct
     6961 gcaacggcgc ccgcgccgat ggtcgcagcg cccggggggc ccgatgccgc cctcgccgag
     7021 gccctccagg atgtcagcca gggacgtctg acaaccgccc tcgccaggat cgaccggctc
     7081 atcgcggcgt atcccacgtt ccgtctggcc cagttgatcc gtggcgacct gctgctggcc
     7141 caggccggcc ccatcagcgg cttcggcaac gttgccgaag ccccgcccga ccgcctcgcc
     7201 gacctgcgcg acgaagccgt ggtgcgcctg aaggccttcc gtaaccgccc gccggccaac
     7261 tacgtgcccc gctacctgct gcagatggag ccggaacaga aatacgccgt cgtcgtggac
     7321 acccagagtg cccgtctcta tctctatcag aacgatgccg gccggccccg ctacgtggcg
     7381 gattactaca tttcccatgg caagctgggt gcagagaaat tccgtgaagg cgacaagcgc
     7441 acgcccatcg gcgtctatta cgtcacctcc agcctgccac gccagaaact caacgacttc
     7501 tacggcagcg gcgccttccc catcagctac ccgaatgaat gggatcgtca gcagggtcgg
     7561 gacggacacg gcatctggtt gcacggcacg cccagcgaca cctattcgcg cccgccgcgc
     7621 gcctctgacg ggtgtgtcgt gctcaccaac cccgatctgg aagccctcgg gggctacctg
     7681 cagatcggca tcacgccggt catcatcagc cacaacatcg aatggctgag cctcgaagac
     7741 tgggccggcg aacgcacggc cctcggcaag gcggtcgagg cctggcgctc ggactgggaa
     7801 agccgtgacg tgaagcgcta cctgtcccat tattccagcc gcttccgcaa caacgaaggc
     7861 ggctacgacg aatggtcgcg acagaagcag ctggtcgcgg ccggcaagtc gtggatcaag
     7921 gtcagcctca atcacctgag catcttccgc aaccccggca agcaggacat ggccgtcgtc
     7981 agcttcgagc aggattaccg cagcaacaac ctgtccaaca cgctgcgcaa acgtcagtac
     8041 tggatgaagg aaggcggtcg ctggaagatc gtctacgaag gcttcgcctg atccaggttc
     8101 ctcttacccg cactcccgga gatcgcccca tgaaatgcgc cctcgccgcc ctcatccttg
     8161 cttcccaggc ccttgtggcc agtgctgcac cccaggttga actgaagacc accgccggca
     8221 cgatcgtcgt cgacctgaac agcgagaagg cgcccaagac cgttgccaat ttcgtcgact
     8281 atgtgaaggc cggccactac aacggcgtga tctttcatcg ggtcatcgac ggcttcatga
     8341 tccaaggcgg cggcttcacc cccgagatga aggaaaaacc cacccgcgcg ccgatccaga
     8401 gcgaatccaa gaacggcctc aagaacgacg ccggcaccct cgccatggcc cgcacccagg
     8461 acccccactc cgcgtccgcc cagttcttca tcaacctcgt ggacaacgcc cccctcaact
     8521 acccctcgcc cgatggctgg ggctacaccg tattcggcaa ggtcgtgacc ggcatggacg
     8581 tggtccgcaa gatcggccag actcccaccg gcaaccgggg cttttaccgg gacgtgcccc
     8641 tcgaagccgt cgtcatccag agcgcccgcg tgctgcccga cgcccccgcg gccaagtaaa
     8701 cacgtttttt cccttccaac aggagcaccc catggctgtc aaactcgtga ccaacttcgg
     8761 cgacatcacc cttgaactgg acgccgacaa ggcccccgtc accgtcaaga acttcctcgg
     8821 ctacgtggaa gccggccact acaacaacac cgtcttccac cgcgtcatca acggcttcat
     8881 gatccagggc ggcggcttcg aacccggcat gaaacagaag gacaccggcg agcagatcca
     8941 gaacgaagcc aacaacggcc tcaagaacga cgctggcacc atcgccatgg cgcgcaccca
     9001 ggcaccccac tccgccaccg cccagttctt catcaatgtg gccgacaacg acttcctcaa
     9061 ccaccgctcc cccgacattc agggctgggg ttactgcgtc ttcggccgcg ttaccgaagg
     9121 catggacgtg gtgaacaaga tcaagggcgt caagaccggc tccaagggct tccaccagga
     9181 cgtgccggtg gaagacgtca tcatcgagcg cgccgagatc ctctgatccg gcctgtcagc
     9241 ggggcttccg gcacatgcgc gtccatttca tctccgatct gcacctgagt gccgaccgcc
     9301 ccgccctcat cgggctgttc gaacgctacc tggccggccc ggcacgtacg gcagaacagc
     9361 tctacatcct cggtgacctg ctcgaatact ggtgtggtga cgacgacacg gatgatccgc
     9421 tggcgcgccg cctgtgcggt gccctgaagg acctttccgc gtccggctgt caggtgagct
     9481 tcatggccgg caatcgggat ctgctcatcg gccccgcctt cgccgagcgt gccggcttca
     9541 cgctgctgcc ggagccgacg ctgatcgagt tggacggaca gccaacactg ctgtgtcacg
     9601 gcgacagcct ctgcaccgat gacgtggcct accaggtcta ccggacccag gtgcgggacc
     9661 cggtctggca gcagggcttc ctggcgcaac cgctggcggc ccgcaagcag ttcatccagg
     9721 gcctgcgcat gaagagcgag gcggccaaga gcggcaaggc ggccgacatc atggacgtca
     9781 atgccgacgc ggtggccgga ctgctgcgcg cccacgccta tccgcggctc atccatggcc
     9841 acactcaccg cccggcaacc catcacctca cggtggatgg gcacgcctgc gaacgctggg
     9901 tcctggccga ctggcatgat gaccagggcg aagtcctgat ctgggacggc gaccgcctgc
     9961 tgcgccagat cctgcgctga aaaaacgaat gcccggacct tgccgggcat ttccgtttac
    10021 cgcagcgccg gcacagaggc cggtttgcct tacaaaaggg ccagcccgcg ggccagatcc
    10081 gctttcagat cctcgacgga ctccagcccg acggccaccc gaagcaggct ttcacggata
    10141 ccggcggcct cccgcgcttc agggctgatc cgtccgtggg tcgtggtggc aggatgggtg
    10201 atggtggtct tggtatcccc cagatttgcg gtaatggaca tcacctgggt gtggtccacc
    10261 acccgccagg cttcgtcgcg gccgccctgc acctcgaagc tcacgatggc accgccgctc
    10321 ttctgctgcc gcaaggccag ttcatgctgg ggatgggacg gcagcccggg gtaatgcacc
    10381 cgcgccacct gcggctgggc ttcgagccag cgtgccagtt cgagcgccgc cgctgactgg
    10441 gccgccatgc ggatggacag ggtctccagg cccttgagga tcacccaggc attgaa
//
LOCUS       DAOQPA010000117        10466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3323_length_10466_cov_18.298146, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000117 DAOQPA010000000
VERSION     DAOQPA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_10466_cov_18.298146"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKX01_14005"
     CDS             <1..308
                     /locus_tag="PKX01_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77291.1"
                     /translation="EPFALAQYVDLREDEQLSQPAFVPLDGGLTVAADDLAVEDEAGL
                     ATPIAYETLVIDPTRPPERPKPGYVLSAAVLERVARFGAAGQAPARTRRRTSTAVTR"
     gene            329..>1653
                     /locus_tag="PKX01_14010"
     CDS             329..>1653
                     /locus_tag="PKX01_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77292.1"
                     /translation="MPEQIAATYRFYSWLRQGLLAGLSGPGASSPATAGRLALPIRLR
                     VNDRAPIEVNVQLYGPGDITGLDAREIIRTEPHAHMTDFEPNYFPAIEFDRPDFPWLF
                     TPAVADGARRLRPWICLIVIPKERATFKTNPQQPLPAITCALEELPDLDQSWAWAHAQ
                     IVAEQRSTPDPSPRATLQQLLNDHPERTLSRLLAPRRLDPNTAYYACLVPTYDVGRKA
                     GLGEPIKPEDEQALTPAWGKNSATGPVTLPVFFQWEFRTGLAGDFESLARRLKAQPLP
                     STVGLRPVDIRTPGWGMPTLPPGAPGSLLDLGGALQTPETQPRPWPDQPRTAFQQALA
                     TILNAPSTMANTSGSPALLGPPLYGQWYVDRDSLPPADQPPHWFRDLNLDPRHRIAAG
                     IGTTVIRKEQEPLMASAWDQLEQERRDRQRLKRAQLAEAVGNSLSKKHFA"
     assembly_gap    1654..1753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1754..2635
                     /locus_tag="PKX01_14015"
     CDS             <1754..2635
                     /locus_tag="PKX01_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77293.1"
                     /translation="TTFAPSFPQPMYEPLRDYAPDLLLPGMDQVPPNTIALLKTNPAL
                     IEAYMVGLNHEMSRELLWRGFPTDQRGTYFRQFWDAGGEELPTTDAEREARFDITQIT
                     TWAADSRLGSHSARGAAVGQMVLLIRGDLLRRYPRAMVYALESVWSADGTRRELGTTE
                     RYPIFRATQSPDITMLGFQLTETDVRGADNKAGGHPGWFFVLQEQPTEPRFGLDVATT
                     YGGTPAHWSDVTWGNLTANEAALKQIVYVPIDGLLKNAVVDQIAWGKNSAQMATITRQ
                     PPFRVAVHARTWLSGRQ"
     gene            2656..3471
                     /locus_tag="PKX01_14020"
     CDS             2656..3471
                     /locus_tag="PKX01_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77294.1"
                     /translation="MPYLAQETTRLQTALQALAAQQAALTTQLTTQQQAVTAAQAQRT
                     NAQSGVTQAQARVAPLQAAAAAADAKVAEAQQDLLDGSEPPQGIPPATWRARLAALRK
                     QLALAQTAATAAHAKVTEAQQGVAQAQAQVQAADRQIAAATAAVQATQAAIAAFQPRR
                     QELQSALSEIERMNVEITRDPMARATVQQVAAQLSARTGTLEESLLVTRFELEDAEAL
                     LASSITRRNELTTLLANLATQIPEAEAQAAAAQEALAAAEAEVTTLLQDGPLG"
     gene            3488..8800
                     /locus_tag="PKX01_14025"
     CDS             3488..8800
                     /locus_tag="PKX01_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77295.1"
                     /translation="MRPIDEGSDIPTRVPPAVLRAWATRLRQLQLAHDRAVARAQAAQ
                     RRAALLIRQRALYQAELSQVNAQITATQDTVTRKRAEVAALSAQVDDHRAARDAATAR
                     LLGTDRLSGTVATGHPLLLFPVRLETRFAARRTGPGTDLLLRVYPDDIHLDSHEPALT
                     EEEERRGKEFWAHVAATPASADRQEQIRQGWQRLTEQFGTPRAAWIAHVLDPAQGGTV
                     TRRDDSWTRAPRTQALPDRWVAIGYRNDAARLTAWGNPIPETVAVGPDPRSTGPVAGD
                     GLPPVDEGMKWITDFDTAESIGMGLRIPLTDEDAQAGFDRIVVLGLKAAWDAATTANK
                     LGQLLDAHHYTGGLALVEQQVPTNNTAEASAGYRSTGRDAADSMRVELGASLIHSGSD
                     GDLLTKTLGLPASVFAHIRGADGAEQRRASLMQSALLAICDSPLLRQLLNATGADLLR
                     EHVAKHVRARGPLPVLRVENQPYGLLPVAALDRWISTTDQDPDKALAAWWRAQRLTRR
                     RQIPQALQTTVESNPVVLLAQEGNTCRYTLREFPEASIQQPPIPKKLLTAELRTLLLT
                     RALSTVHDPAWDELATLPEPIRQLLLAEAMDVMTYRLDAWGTSLATRRLAVMRQAPTG
                     IRLGAYGWVEQVRRATPLQAVPTPPAGVTTPVSRSAQNKGFVHAPSLGHAAAAAVLRS
                     GYLSQASRASGAAPFAVDLSSERVHRAKWLLDGVRQGQSLSALLGYRFERLLHERSLD
                     RYIQRFRALTSFTDTDRFADIRDTLTRTEKLAQEVTLLTAQRDQALRRAEDARGLKAE
                     RERREQTYRAALGAIATLAQQAQAAQTQVTQAAQVLAQQQAAKPEGKVTQPAARRYAI
                     QLLEPRDLDDWENRVEQLTQAQVTAVAQAAAAQQAVSAQEGSRLLAERAQAKLLNAAD
                     PDSIPAAQQMATGQETLAAEFDRQALAKEGGQRGKVMTDLLAARATLTTRLAAQWNDA
                     LKSLPAAAVVDGLALHRRWTASQQRQAPQTPWDVTTVPFGNATLGFPPVGSPDFTALV
                     DVLKALDDLVDSVGDSVVAESVYQLVQGNPLRSGATLDAIAAGEVPPPELEVIRTPRS
                     GIGLTHRLCALFPATDGTAPSGWPTIAPSARALAEPILNAWVATLLPNPAQVRCKADY
                     MNQETGQIYQTVESALTALGLAPLDAVYLAEGNSRAQQAELEQRWRFALQQTRPTAVP
                     ADAVIRLQFGRDNGWGAEIVSVTEWCEVATTVRRLLSNARSLDGRDLSLPESAAESGL
                     DHEEFSDRATRALQALTEAQRLLTGLLPAPPSDELTANLDDVRRALFGLARFGIPSAV
                     PMDIGGTGTEARSVLLTQSRSVLLEAQRRLSRVAASEQDFARANATPEERRDHDLARF
                     RIIFGQDFLALPRVTATNAAQLNETFAASLSLQGQDPLAAVTWFQRAAYVRPGATRLN
                     EALLYGETVGSAALRFQVGQLPFQAQDRWVALPPKPDQPFSRGRVSLVAQMSSAQPLR
                     FDQPFAGLLIDEWGETVPSSSETTGVAFHYDQPNNAPPQALLLAVPSDRRATWDLNSL
                     EAVLQETMDLARLRAVAPDSGDELIWVEDQLPEGAAPLGDGENWTWIRMKPEPLSGTR
                     AHQSVIAAGMHQHFFQGAKSPLFVNVGDRLFAHIYLDPARMPRQVMLQWHDGNWEHRA
                     YWGENLIPWGTDNTVSRQYMGPLPPPGRWVRLEVPAATVGVEGRIVGGMAFTLFDGMA
                     TWDRAGKRAVQPVGSGEQDPSAPALLFTGGTLDFTGVIDPAIGA"
     gene            8803..>10466
                     /locus_tag="PKX01_14030"
     CDS             8803..>10466
                     /locus_tag="PKX01_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77296.1"
                     /translation="MASRIRLDLSTQNPDLQDGLQARIHDPLWMLGRQWQFGEFNGAD
                     AGSPAAAQVIVDTAAVTRYQPGPRAGTLPARPYATNNLALETLVEAEPVVDGSRQNWR
                     LAAESGRHLLRLLETARLGPSRAQWLGSAYALAAPTSEQAKQIDAASLSFIRVLTGRT
                     IDGLRFASRIRTLQAANQLNDIFGDAPLNQVPDVDRAKVLGIVTTWLTWVESLFQQGS
                     ASSAWIPERMEYALSVSAKTASSEVVLTAPEYLDGRLDWFSFTTSPGNGLGATESRTT
                     LTEAFLPAPVSFRGMPSARLWEFEDGAVNFASVQAAPQDLARLLLVKFALEYSNDWFL
                     LPLDLAVGTLSQIRALVVTNTFGERFLIPHASEVDGPASPWRMFSLTNDTQKLFFLPP
                     VLGPMLESQPIEDLSLLRDEMANVAWAVERVVESAAGRPLDRHEAYHETLAEQPPSPT
                     ADADGAPLVYRLGTTVPDYWIPLLPVKEGTTLRLKRGVLPAFGEGGIQGLQLPKGRLL
                     EPNRELLLYEEEVPREGARVTRSYQYARWIDGTTHLWIGRRKDPGRG"
BASE COUNT         1953 a         3527 c         3226 g         1660 t
ORIGIN      
        1 cggagccctt cgccctggcg cagtatgtgg acctgcgcga ggatgagcaa ttgtcacagc
       61 cggcgtttgt gccgctggac ggcggtctca cggtcgcggc cgacgatctg gccgtcgagg
      121 acgaggcagg cctggccacg cccatcgcgt acgagacgct cgtcattgat ccgacgcgcc
      181 cgccggagcg gccgaaaccg ggctatgtgc tctcggcggc ggtgttggag cgggtcgccc
      241 gcttcggcgc agccgggcag gctcccgctc gcacacggcg acgaacatct acagctgtca
      301 cgaggtaatt caaccggaat gaagcaccat gcctgaacag atcgctgcca cctatcgctt
      361 ttattcctgg cttcgccagg gactgctcgc gggtctctcc ggacccggcg cttccagccc
      421 ggccacggcg ggacgcctcg cgttacccat ccgcctcagg gtcaacgacc gggcgcccat
      481 cgaagtgaac gtgcaactct acggccccgg cgacatcacc ggcctggacg cgcgcgagat
      541 tattcgcacg gaaccccacg cccacatgac cgacttcgag ccgaattatt ttccggccat
      601 cgaattcgat cgccccgact ttccctggct cttcacgccg gcagtggcgg acggtgcccg
      661 gcgcctgcga ccatggatct gcctgatcgt catcccgaag gaacgagcga cgttcaagac
      721 gaacccgcag cagccgctgc cagcgattac ctgcgcgttg gaagagttgc cggacctcga
      781 tcaatcctgg gcctgggctc acgctcagat tgtggcagag caacgctcga cgcccgatcc
      841 ttcaccgcgc gcaacgctgc aacaacttct caacgaccat ccggaacgca cgctctcgcg
      901 gctgctggct ccacgccgcc tcgatcccaa taccgcctac tatgcctgcc tcgtgcccac
      961 gtacgacgtg ggaagaaaag caggcctggg cgagccgatc aagccggaag atgagcaggc
     1021 gctgacgccg gcctggggaa aaaacagcgc gaccggcccg gtcacgctgc cggtgttttt
     1081 ccagtgggaa tttcggaccg gtctcgcggg ggatttcgaa tcacttgcgc gtcgattaaa
     1141 agctcagcct ctgccatcca cagtgggcct acggccggtg gatatacgca ctcctggctg
     1201 gggcatgccg accctgccgc ccggcgcgcc gggcagcctg ttggatcttg gcggagccct
     1261 ccagactccg gaaacacaac cacgtccctg gcccgatcaa ccgcgcaccg cgtttcaaca
     1321 ggcgcttgcc accatcctga acgcgccgtc aacgatggcc aatacgagcg gatcgcctgc
     1381 gttgcttggc ccgccgctct atggacaatg gtacgtagac cgggacagcc tgccgcccgc
     1441 cgaccaaccg cctcactggt tccgcgatct gaacctggat ccgcgccacc gcattgccgc
     1501 cggtatcggc accacggtga tccgcaagga gcaggagccg ttgatggcct cggcctggga
     1561 tcaactggaa caagagcggc gcgatcgaca gcggctcaag cgcgcgcagt tagccgaagc
     1621 ggtcggcaac agcctgtcga agaaacactt cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnacgacct tcgcgccaag ttttccgcag ccgatgtacg aacccttacg
     1801 cgattacgcg ccggacctgt tgttacccgg catggaccag gtgccaccaa acaccattgc
     1861 gctcttgaag accaatccgg ctttgatcga ggcctatatg gtcggcctca atcacgaaat
     1921 gagccgtgaa ctgctctggc gtggatttcc gaccgatcaa cgcggcacct acttccgaca
     1981 attctgggac gccggcggcg aggagctgcc cacgaccgac gcagagcgcg aagcccgttt
     2041 cgacatcaca caaatcacca catgggcggc ggacagccgt ctcggttcgc acagcgcacg
     2101 aggcgcagcg gtcggccaga tggtgctgct catccgcggt gacctcctgc gccgttatcc
     2161 ccgcgcgatg gtctatgctc ttgaaagcgt ctggtctgcc gacggcacca ggcgtgagct
     2221 gggcacaacg gaacgctatc cgatttttcg cgcgacgcaa tcaccggaca tcaccatgct
     2281 cgggtttcag ctgaccgaaa cggacgttcg cggagccgac aacaaggccg gaggacatcc
     2341 cggttggttc ttcgtgctgc aggaacaacc gacggaaccg cgattcggcc tggacgtcgc
     2401 cacgacgtat ggcgggacgc ccgcccattg gtccgatgtg acgtggggaa acctgacggc
     2461 gaatgaagcc gcgctcaaac agatcgtgta tgtgccgatc gacggtctgc tcaaaaacgc
     2521 cgtcgtggat cagattgcct ggggaaagaa ctcggcgcag atggcgacca tcaccagaca
     2581 gcccccgttc cgcgtggcgg tccatgccag aacctggctg tctggccggc agtaacggcg
     2641 gaaagaggcg agaccatgcc ctacttggca caagaaacca cccgactcca gacggcgctg
     2701 caggccttgg ctgcacagca ggccgcgctg acgactcaac tcactaccca gcagcaggcc
     2761 gtgacggcgg cccaggccca acgcacgaac gcacagagcg gcgtcacgca agcgcaggcg
     2821 cgcgtcgcac cgttacaggc agccgctgcc gcagccgacg ccaaggtcgc agaagcgcaa
     2881 caggatctgc tcgatgggtc ggaaccgccg caaggcatcc ccccggccac gtggagggcg
     2941 cgcctagccg cgctgcgaaa acaacttgcc ctagcccaga ccgctgcaac cgccgcccat
     3001 gccaaagtga ccgaggcgca gcagggcgtt gcgcaggctc aggcgcaagt gcaggctgcg
     3061 gatcgccaga ttgccgcggc aacagctgcg gtgcaagcca cgcaagcggc gatcgcggcg
     3121 tttcagcctc gccggcagga actgcaatcg gccctcagtg agatcgagcg catgaatgtg
     3181 gagatcacgc gcgacccgat ggcgagagcc acagtgcaac aggtggcggc acagctctcg
     3241 gcccgcaccg ggacattgga agaatcactg ctggtgacgc gcttcgagtt ggaggatgcg
     3301 gaggcgctcc ttgccagctc gatcaccaga cgcaatgaac tgacgacact cctggccaac
     3361 ctggccacgc agattccgga agctgaggcg caggcagcgg cagcccaaga agcgctggcg
     3421 gccgccgaag cggaagtcac gacattgtta caggatgggc cactaggttg atgaaaggac
     3481 caacgcagtg agaccgatcg acgaaggatc cgatatccct acgcgggtgc ccccggcggt
     3541 gctcagggcc tgggcaacca gactccggca attgcagttg gcgcacgacc gggcggttgc
     3601 ccgggcgcaa gccgcgcaac ggcgggcggc cctgctcatc cggcagcggg cgctgtatca
     3661 ggccgagttg agccaggtga atgcacaaat cacggcgacg caagacaccg tcacgcgcaa
     3721 gcgcgccgag gtggccgcat tgtcggcgca ggtcgacgat catcgcgccg cccgtgatgc
     3781 cgcgacggcc cgccttcttg gcacggatcg cctcagcggg acagtcgcaa ccggccatcc
     3841 cttgctgctg ttcccggtgc gcctggaaac cagatttgca gcgagacgca cggggccggg
     3901 taccgatctg ttgctccgcg tctatcccga cgatattcat ctcgacagcc acgaaccggc
     3961 cttgacggag gaagaggaac gccgcggcaa ggaattctgg gcccatgtgg ccgccacccc
     4021 ggcaagtgcg gaccggcagg aacagatcag gcagggctgg caacgactga ccgaacaatt
     4081 cggcacgcca cgcgccgcct ggatcgccca tgtgctcgat cccgcgcaag gcggaacggt
     4141 cacccgccgg gacgacagct ggacacgcgc gcctcgcaca caagccttgc ccgatcgctg
     4201 ggtcgccatc ggctatcgca acgatgccgc gcgcctcacc gcctggggca atccaattcc
     4261 agagaccgtg gcggtcggac cggacccgcg tagcaccggc cccgtcgccg gcgatgggtt
     4321 gcctcccgtc gacgaaggca tgaagtggat cacggatttc gacacggccg aatcgatcgg
     4381 gatgggtcta cgtattcctc tcacagacga agacgcgcag gcggggtttg accgcattgt
     4441 ggtccttggc ctcaaggcag catgggacgc ggcgaccacg gcaaacaaat taggccaact
     4501 cctcgacgcg catcactaca ccggtggcct ggcccttgtg gaacaacagg tgcccacgaa
     4561 caacacggcg gaagcctctg ccggatatcg ctcgaccggg cgcgacgccg ccgacagcat
     4621 gcgcgtcgag ttgggcgctt ctttgatcca ctccgggtcc gatggcgacc tgctgacgaa
     4681 gaccctgggc ctccctgcgt ccgtctttgc ccacattcgc ggtgccgatg gagcggaaca
     4741 gcgccgggct tcgctcatgc aatcagccct gcttgcgatc tgcgacagtc cgctattgcg
     4801 gcaactcctc aacgcaaccg gagcggacct gttgcgtgag catgtcgcga agcacgtccg
     4861 cgctcgcggg cccctccccg tcctgcgagt cgagaaccag ccctatggcc tactgccggt
     4921 ggcggctctc gaccggtgga tatcgacgac cgatcaggat ccggataagg cgctggccgc
     4981 ctggtggcgc gcccagcggc tgacacgtcg ccgccagatt ccgcaggctc ttcagaccac
     5041 ggtggaatcc aaccccgttg tgctcctcgc acaggaaggg aacacctgcc ggtacactct
     5101 gcgcgaattt cccgaggctt ccatccaaca accgccgatc ccgaaaaaac tcctgacggc
     5161 ggagctccgg acactcctgt tgactcgcgc cctttcgact gtacacgacc ccgcgtggga
     5221 cgaattggcg accctgcctg aaccgattcg gcaactcctg ctcgcggagg ccatggatgt
     5281 catgacgtat cgcctcgatg cgtggggcac gtccctggcc accagaaggt tggccgtcat
     5341 gcggcaggcg cccaccggta tcaggctcgg tgcctatggc tgggtggaac aggtccgccg
     5401 tgctacgccg ctgcaagcgg ttcccacgcc gccggccggt gtcacgacac cggtctcgcg
     5461 ctcggcccaa aacaagggct ttgtccatgc gccttcgctg gggcacgccg cggccgccgc
     5521 cgtgctgcgg agcggctacc tgtcgcaagc gtcgcgtgca tccggcgccg ctccctttgc
     5581 ggtcgacctg tcgtctgaac gggttcatcg cgccaaatgg ctgctcgacg gcgtgcgcca
     5641 ggggcagtcc ttgagcgcgc tgctggggta tcgattcgag cgactgcttc atgaacgcag
     5701 cttggaccgc tatatccaac gcttccgcgc cctcacgagc tttaccgaca ccgaccgctt
     5761 cgccgacatc cgggacaccc tcacgcgcac cgaaaagctg gcccaggaag tgacgctgct
     5821 gacagcgcaa cgcgaccagg ccctgcgccg ggcggaagac gctcgtggtc tgaaggctga
     5881 acgggaacga cgcgagcaaa cctatcgtgc cgctctggga gcgattgcca cgctcgcgca
     5941 acaggctcaa gccgcgcaga cccaggtgac gcaggccgct caggtactgg cgcaacaaca
     6001 ggcggcgaaa ccggaaggca aggtcaccca acctgcggcg cgccgttacg ccatccagtt
     6061 gctggagcca cgagatttgg acgactggga aaatcgcgtg gaacaactga cccaggcgca
     6121 ggtcaccgcc gtggcccagg ccgccgccgc ccaacaggcc gtcagcgcgc aggaaggctc
     6181 acgtctgctc gcggaacggg cccaggccaa gttactcaac gccgccgacc cggattcgat
     6241 tcccgctgcg caacagatgg caaccgggca agagacgctc gccgccgagt ttgaccgcca
     6301 ggcgctcgcg aaggaaggcg gacagcgcgg caaggtcatg accgatctcc tcgccgccag
     6361 ggcaaccctc accacgcgcc tcgccgcgca atggaatgac gcactgaagt cgctgccggc
     6421 cgccgccgtg gttgacgggc tggccctgca tcgtcgatgg acggcgagcc agcaacgcca
     6481 ggcaccgcaa acgccctggg atgtcaccac cgtgccgttc ggtaacgcca ccttgggatt
     6541 tccgccagtc ggcagccccg actttacggc gctggtggac gtattgaagg cactcgacga
     6601 tctggtcgac tccgtgggag acagcgtcgt agccgagagt gtctaccagc tcgttcaagg
     6661 caatccgctc cgctccggtg ccacgttgga tgccatcgcc gcgggtgaag tgccgccgcc
     6721 ggagttggag gtgatccgga cgccgagaag cggcattgga ctgacgcatc gcctctgcgc
     6781 actgtttccg gcgactgacg ggacggcgcc ctccggctgg cctacgatcg ccccgtccgc
     6841 acgcgccctg gccgaaccga ttctcaacgc ctgggtggcc acactcctgc cgaacccggc
     6901 acaggtccgt tgtaaggcgg actacatgaa ccaggaaacc ggacagatct atcagacagt
     6961 cgagagcgcg ctgactgcgc tcggattggc gccgctggat gccgtctacc tcgccgaagg
     7021 caacagccgc gcacagcagg cggaactgga acaacggtgg cggttcgcgc tgcagcagac
     7081 ccgtcctacg gcggtgccgg cggatgcggt cattcgattg caattcggcc gcgacaatgg
     7141 ctggggcgcg gaaatcgtca gcgtgacgga atggtgcgag gtcgcaacca cagtgcgccg
     7201 gctgctcagc aacgcccgat cactcgatgg ccgcgacctg tcgttacccg aatcggccgc
     7261 tgaatccggc ctggaccacg aagagttctc cgaccgtgcc acacgcgcat tgcaagccct
     7321 gaccgaggca caacgtctgc tgaccggcct actccccgcg ccgccgtcag atgaactgac
     7381 tgccaacctc gacgacgtgc gccgcgcgct gttcggtctg gcacgtttcg gcataccgag
     7441 cgcggtgccg atggacatcg ggggcacggg aacggaagcg cggtcggtgc tgctgactca
     7501 atcacggtcg gtgctcctcg aggcacaacg gcgactcagc cgcgtggccg catcggagca
     7561 ggactttgcg cgggcgaatg ccacgccgga agagcggcgc gatcacgacc tcgcgcgatt
     7621 ccgcatcatc ttcggtcagg atttcctggc gctccctcgc gtgaccgcga ccaacgccgc
     7681 gcaactgaac gagacgttcg ccgccagcct ttcgctccag gggcaagatc ccctcgccgc
     7741 cgtcacgtgg ttccagcggg ctgcgtatgt caggcccggc gcaacacgcc tgaacgaagc
     7801 cctgctctat ggcgaaaccg tcgggagcgc ggccctccga ttccaggtgg gacaacttcc
     7861 ctttcaggcc caggatcgct gggtggctct gccgccgaaa cctgaccagc ccttctcacg
     7921 cgggcgcgtt tcgctcgtgg cgcagatgtc gtcggcacaa ccgcttcgct tcgatcaacc
     7981 gttcgccggc ctcttgatcg acgaatgggg tgagacggtg ccgagttcca gcgaaacgac
     8041 gggagtggcc tttcattatg atcagcccaa caatgcgcct ccccaggcac tgctcctcgc
     8101 cgtgccgtcc gaccggcgtg cgacctggga cctgaacagc ctggaagccg tcctgcagga
     8161 gacgatggac ttggccagac tccgcgccgt tgcgccagac agcggcgacg aattgatctg
     8221 ggtggaggac caactgccgg aaggcgctgc tcccttgggg gacggtgaga actggacctg
     8281 gattcgcatg aaaccggaac cgctctccgg cacgcgcgcc catcaatcgg ttattgcggc
     8341 cggcatgcac cagcattttt tccagggcgc caagagcccg ctgttcgtga atgtggggga
     8401 ccgcctgttc gcacatatct acctcgatcc cgcgcgtatg cctcgacaag tcatgttgca
     8461 gtggcatgac ggcaattggg agcatcgtgc ctattggggc gaaaacctca ttccctgggg
     8521 cacagacaat accgtcagcc ggcaatacat gggcccgttg cccccgcccg gccgatgggt
     8581 tcgtctcgaa gtgccggcgg caaccgtggg cgttgaagga cggatcgtcg gcggcatggc
     8641 ctttacgctg ttcgacggga tggcgacgtg ggatcgcgcc ggcaagcgcg cagtacagcc
     8701 ggtgggctcc ggtgaacagg atccctcggc accggccctg ctgtttaccg gcggcaccct
     8761 cgacttcacc ggcgtgatcg atccagcgat aggagcatag gcatggcctc ccgaatcaga
     8821 ctcgacctca gtactcagaa ccccgacttg caggacggat tgcaggcgcg catccacgac
     8881 ccgctctgga tgctgggccg tcaatggcag ttcggcgaat tcaacggagc cgacgccggc
     8941 tcacccgccg ccgcgcaggt gatcgtggac actgccgccg tcacccgcta tcaaccgggg
     9001 ccgcgcgccg gcacgcttcc ggccaggccc tatgccacca acaacctggc gctggagacg
     9061 ctggtggaag cggagccagt cgtagacggc tcgcgccaga attggcgcct cgcagctgaa
     9121 tccggacggc acctgctccg cctgctggaa acggccaggc tcggcccctc acgcgcccag
     9181 tggttgggaa gcgcctacgc attggccgcc cctacctcag agcaggccaa gcagatcgac
     9241 gctgcgagcc tctcgttcat ccgcgtcctg acaggccgca ccattgatgg gctacgattc
     9301 gcctctcgaa ttcgcaccct gcaggcggcc aatcaattga atgacatatt tggggacgcg
     9361 ccgttgaacc aggtgccgga cgtggatcgc gccaaggtgc tcggcatcgt caccacctgg
     9421 ctgacgtggg tggaaagtct ctttcaacag ggcagcgcct cctcggcctg gattccggaa
     9481 cggatggaat acgccctcag tgtctctgcc aagacggcct cgagcgaagt ggtgttgact
     9541 gcgcccgagt acctcgatgg acgactggac tggttttcat tcacgaccag tccaggcaat
     9601 ggtctgggcg ccacggaaag ccgcaccacc ctcacggaag cgtttctacc cgcccccgtc
     9661 tcctttcgcg gcatgccctc cgcgcgcttg tgggaattcg aagacggcgc cgtgaacttc
     9721 gcaagcgtcc aggccgcgcc gcaggacctg gcccgcctgc tgcttgtgaa gttcgcactg
     9781 gagtacagca acgattggtt cctgctcccg ctcgaccttg ccgtcggcac actctcacag
     9841 atccgcgcgc tcgtcgtgac caacacattt ggcgaacgct tcctcatccc ccacgcgagc
     9901 gaggtggatg gaccggcctc gccctggcgc atgttcagcc tcacgaacga tacgcagaag
     9961 ttattcttcc tgccgccggt attgggcccg atgttggaga gtcagccgat agaagacctg
    10021 tcgttgctgc gagacgaaat ggccaatgtg gcatgggcgg tcgagcgcgt cgtcgaaagc
    10081 gccgccggcc ggccgctcga ccgtcatgag gcctaccatg agacgctggc ggaacagccc
    10141 ccctccccca ccgctgacgc tgacggcgca ccactggtct accggctcgg caccacggtg
    10201 ccggactatt ggattcccct gctccccgta aaagagggca ccaccctgcg gctcaaacgc
    10261 ggcgtgcttc cggcattcgg cgagggcggc attcagggtc tgcaacttcc gaaggggcgt
    10321 ctactcgaac cgaaccgcga gttgctgctg tatgaggaag aagtgcctcg cgaaggagct
    10381 cgcgtcacga gaagctatca atacgcgcgc tggatcgacg gcacgaccca cctctggatc
    10441 ggccgccgca aagacccagg ccgggg
//
LOCUS       DAOQPA010000118        10322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3400_length_10322_cov_18.584689, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000118 DAOQPA010000000
VERSION     DAOQPA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_10322_cov_18.584689"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PKX01_14035"
     CDS             complement(<1..1025)
                     /locus_tag="PKX01_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ77297.1"
                     /translation="MTSTLRGTCLALLALTGLSLLPGAARADDAVGGRVIVKFRSSSA
                     LKQIAAAGRVQSLAQRLGVTATLIGQPAPELHVMHARGISTAALAARLAQESDVEFAV
                     PDRLKQAAALPNDPLFSAQWMLQTPDTTHPAAIDAVGAWTLSAGSSGVVVAVVDTGVR
                     LDHEDLMSRLLPGYDFISDDLNAGDGQPGRDADPADPGDFVSSADLANSAFTQLCPGV
                     SRASSSWHGTQIAGIVAAEANNSLGIAGVAGATRVLPVRALGKCGGFDSDIIAGMRWA
                     AGLSVPGVPANTHPARVINLSLAGSGSCSAAYASAIAEIRAAGTLIVAAAGNETGPVE
                     EPANCAGV"
     gene            complement(1022..1354)
                     /locus_tag="PKX01_14040"
     CDS             complement(1022..1354)
                     /locus_tag="PKX01_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77298.1"
                     /translation="MPPTRAALAATALIVLMATACADAPTRTATGVRILVEFTTPTDG
                     AAPATRAFLEATGGVPVRYVTAVSGRTHAYRLACPPQDDTCSAALDALRRAPAIRSVT
                     PDHLKDTP"
     gene            complement(1407..3053)
                     /locus_tag="PKX01_14045"
     CDS             complement(1407..3053)
                     /locus_tag="PKX01_14045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77299.1"
                     /translation="MNALWPASLRFRLLVASLVVGSISLALLIAHSARLIEASLLGQL
                     NRQLEQTRPLLNAALNAPLAERDYATLRQILHEARTPEMLTYLVLLDHRGEVIASEGR
                     PASTPLPAIDPTAQFDLDRPDRVFHTEVHIDLAGQALGTLRFGLSTRFLHDERQRLIR
                     ESLELGAAVLLVALFALGWVSLWLTRRLARLTDASRQLAVGHYDVELESSGSDEVDQL
                     STAFLRMAATIKVRVEELVLSEALQRRYYEHARDERARLASLLSALKTGILFVGNDGI
                     VVYYNATFGQIWRIPPEAGLVHQPALRLRPYVERLLDDTGSFMAIPLADADLAEFRLL
                     DHRIVTQTTSPVLDETGTSVGRIWIYEDITHQRQTAELADQAERDALTGLYNRRGFDL
                     ALDRMARTARERNANLALCFIDLDGFKRINDCYGHRAGDAILVQVASEFQAQVRRREC
                     LARVGGDEFAVLVPDVSEEEITLLAERLVKAIAGMPIRYTGQDLRLGCSVGIAIYPWH
                     TRQPDDLMACADAAMYLAKDAGKNRWCMLPTAPPRHAGDT"
     gene            complement(3050..3889)
                     /locus_tag="PKX01_14050"
     CDS             complement(3050..3889)
                     /locus_tag="PKX01_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HMZ77300.1"
                     /translation="MPALLRALILLSLALCARVASADGMRDLEIGVLPNLSTRVLMTQ
                     YAPLRDALARSLNRPARIATAPDWSRFTRRGLEGDYDLIVTAAHLARLQQVEAGLIPV
                     GRFTPDIPALLVTRGDAPVSRLTDLRGKIVALPNVHSLVALQSLHRLAEAGLRPGQDL
                     RLISVPTDDSVGNLLLRGDATAAVLSGGEFRMIPEDIRARLKVVQTVAEVPGFIVLAS
                     PRLDADALERLRRCLESFPGSSDGRRFAELTGFSGLTTVDDALMKSLDIHLPDARQIL
                     QAP"
     gene            4083..4883
                     /locus_tag="PKX01_14055"
     CDS             4083..4883
                     /locus_tag="PKX01_14055"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HMZ77301.1"
                     /translation="MTQPSPILSALYRYPVKSMAGDALNASAVSRLGLPFDRMWMVAD
                     RSGRFVTGRQLPQLVRVRTEPSADGVTLTMAGREALFVPNEVFTEEVSAVVWGSLFPA
                     WGGAAAADAWLSDALGAPLRFLWTGAQTARRVRVEPDVPLSFADGFPLLLIGQSSLDD
                     LSARVGRPLEMARFRPNLVVSGAEAFAEDGWTRIRIGTVSFRLVKPCERCVFTTVDPV
                     TGEKSLDQEPLRTLARYRKTADGVLFGQNVIADGAGELVCGMPVDVLE"
     gene            5079..6050
                     /locus_tag="PKX01_14060"
     CDS             5079..6050
                     /locus_tag="PKX01_14060"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMZ77302.1"
                     /translation="MFKKFKNRVRGALGLLLLGFAALAMADDAPPTPEELAKIRTANA
                     ECFKCHSEAGLRTPVKEGMDLAKLRTLLVNPDTYAASDHGEMACSKCHGDGFDEHPHA
                     PKAKEDLAECQDCHARKAMRIERQFDKSVHAENLSDRFSCATCHNAHTMSFAAKLRDP
                     HKIVAQDNKICLDCHDNDEAFATMAPDKKKRPAIDDIHKWLPNTRLHWQAVRCIECHT
                     PTDDKLSLSHEVQNKDKAEKKCVTCHNPDSSLKVRLYRHLAKEEQQKYGFLNSVILSN
                     SYVVGATRNPTLDLLLIGAFVATVLGVLGHGLLRIITTRIRRSKKND"
     gene            6043..6657
                     /locus_tag="PKX01_14065"
     CDS             6043..6657
                     /locus_tag="PKX01_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMZ77303.1"
                     /translation="MTKRVYMLAPWIRLWHWGNALAIITLSITGISLHFSGSELALVE
                     FSMAARIHNVAGVCLAVLYSVFVIGNIVTGNWWQYVPKPPGVLGRCLKQMQFYGSGIF
                     KGEEEPFPPTPEVNFNALQAVTYWSIMYLVVPTVIVTGLIFLYPQFAPDQIFGMDGLL
                     PIALVHYLAAAVILLFMVSHIYLGTMGPKVSSLFKMMITGWYEH"
     gene            6704..7048
                     /locus_tag="PKX01_14070"
     CDS             6704..7048
                     /locus_tag="PKX01_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ77304.1"
                     /translation="MKIRTVCLATAALATALSAGSALALDADAAQALAKKEGCTKCHA
                     VDKKKEAKSLTEISKSLKGKADAEAKLLHHLTSGEMVKFEDGKEEEHKILKTKDKAEI
                     KNLTDWILSLGK"
     gene            7120..8106
                     /locus_tag="PKX01_14075"
     CDS             7120..8106
                     /locus_tag="PKX01_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HMZ77305.1"
                     /translation="MKIFRKLFASCAVFGALVAAQASLAADAPATPAAKPAAKDLVLK
                     GDAKCTSCHDETDEPKLLHIGKTKHGSVADGRTPSCTNCHGESDLHTNNPAKLKERPP
                     TDRNFGKLSKLSAEDKSAACTTCHQGGKHINWNTSAHANRDVSCTSCHQIHSPKDKVR
                     DKRQQAEVCYTCHKQQRAEANKPSHHPIPEGKMTCSDCHNAHGSAGQKMLVKDTTNAT
                     CFTCHAEKRGPFVHNHQPVVEDCGNCHNPHGTTAEGMLKSRQPFLCQQCHGDSSHPGN
                     VPGVRSLMPNAVSTNIGPGMAQARGCMNCHTNIHGSNSPSNATSSGPTRFFR"
     gene            8122..10317
                     /locus_tag="PKX01_14080"
     CDS             8122..10317
                     /locus_tag="PKX01_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HMZ77306.1"
                     /translation="MSTQQQFRQTALVIALSAVFPLSSAWAEDDDVAQLISPNVTEVT
                     VNVPWVNEVNPLYRQYNGINNQGVNANLDLNIVRRSDDAEWFRLNARNLGLSTQEFGV
                     SMEKQGDWALGLNYDQIPRYAPYAISTAVSGIGTNTITQPSYANSAFTSGSLTNPNLY
                     DVTLKTERDIVTLNASKFVAKDVKLSFMFKNEEKSGLRMDGVRGVAGTNGSTGGTANR
                     YSGFLFSPEPIGQTHKQFEATLDFTNEKYQITAGFYGSFLNTRDNSLNVIPGTNTQLV
                     TTTAPLLSPIALAPDNSMQQFFVSGAYNFSPDTRANLKVSYSDARQNDGFFSGQPTMT
                     GIGTSLNGEVRTTEVFSSVTSRINKNLKLLASWRYEDKVDKTPIRQYVPTSFGEPYNN
                     PESHTANWGKLEADYRLGGGYSVTAGVDYTNKASLEWERNKVSETTTRLAVRKTMSDA
                     INGTLSVAHSERTGSEWTTGSAPPIYPVYLADRKRNKVRGMVDWSATEALSLQLAYEA
                     YFDDYSKSLYGLDNGQGQVYSVDGTYQFNDTWKLNAWYSRQNGESNQNSLGAVCSSGN
                     NSNCTLNTYTNRTAPTDFSQWIDWSAKIKSNSDQVGFGLKGRIQKVDVGMDFLYARDR
                     NQQTLSGLPATTSTARSGTAPNYTYTPTALAAGMGVLPDTSYTQNTVRLNGTYPLSKA
                     TKVRLDYIYDLRKMDDYTWSNWVYADGTKVYVKPEQTTQIIGVSLSHQF"
BASE COUNT         1824 a         3305 c         3589 g         1604 t
ORIGIN      
        1 acgccggcgc agttggcggg ctcttcgacg gggccggtct cgttgccggc ggcggcgacg
       61 atgagggtgc cggccgcgcg gatctcggcg atggcgctgg cgtaggcggc gctgcagctg
      121 ccgctgccgg ccaggctcag gttgatgacg cgggccggat gggtattggc cggcacgccg
      181 ggcacgctga ggccggcggc ccagcgcatg ccggcgatga tgtccgagtc gaagccgccg
      241 cacttgccca gcgcgcgcac gggcagcacg cgggtggcgc cggccacgcc ggcaattccc
      301 agggaattgt tggcttcggc ggcgacgatg ccggcgatct gggtgccgtg ccaggagctg
      361 ctggcgcggc tgacgccggg acagagctgg gtgaaggcgc tgttggcgag gtcggcgctg
      421 ctcacgaagt cgccgggatc cgcggggtcg gcgtcgcggc cgggttggcc gtcgccggcg
      481 ttgaggtcgt cgctgatgaa gtcgtagccc ggcagcaggc ggctcatcag gtcttcgtgg
      541 tcgaggcgca cgccggtatc gaccaccgcc accaccacgc cgctgctgcc cgcgctgagc
      601 gtccatgcgc cgaccgcgtc gatggcggcg gggtgcgtgg tgtcgggcgt ctgcagcatc
      661 cactgggcgg agaacagggg gtcgttgggc agggcggcgg cctgcttgag gcggtccgga
      721 acggcgaact ccacgtcgct ttcctgggcg aggcgggcgg cgagggctgc ggtgctgatg
      781 ccgcgggcgt gcatcacgtg gagttccggc gcaggctgcc cgatgagggt ggccgtcacg
      841 ccaaggcgct gggccaggct ctggacacga ccggctgcgg cgatctgctt gagggcggaa
      901 ctgctgcgga acttgacgat gacgcggccg ccgacggcgt cgtcggcgcg ggccgcccca
      961 gggagcaggc tcaggccggt gagggcgagc agggccaggc aggtcccgcg gagggtggac
     1021 gtcatggggt gtccttgagg tggtcggggg tgacgctgcg gatggccggc gcgcggcgca
     1081 gggcgtcgag ggcggcgctg caggtgtcgt cctggggcgg gcaggcgagg cgataggcgt
     1141 gggtccgccc ggacaccgcc gtcacgtagc ggaccggtac accgcccgtg gcttcgagga
     1201 aggcccgcgt cgctggcgcg gcgccgtcgg tcggggtggt gaattcgacg aggatgcgca
     1261 cgccggtcgc ggtgcgggtc ggtgcgtcgg cacaggccgt cgccatcagg acgatcaggg
     1321 cggtggccgc gagggcggcg cgggtcgggg gcatggcagc tccgcggaac atgcagcccg
     1381 cgtactgtag caccgcgccg cgacgatcag gtgtcgccgg cgtgacgggg cggtgcggtg
     1441 gggagcatgc accagcggtt cttgccggcg tccttggcca ggtacatggc ggcgtcggcg
     1501 caggccatca ggtcatcggg ctggcgggtg tgccaggggt agatggcgat gccgacgctg
     1561 cagccgaggc gcaggtcctg cccggtgtag cggatcggca tgccggcgat ggctttgacg
     1621 aggcgctcgg cgaggagggt gatctcctct tcgctgacgt cggggacgag gaccgcgaat
     1681 tcgtcgccgc cgacgcgggc gaggcattcg cggcggcgca cctgggcctg gaactcgctc
     1741 gccacctgca ccaggatggc gtcgccggcc cggtggccgt agcagtcgtt gatgcgcttg
     1801 aagccgtcca ggtcgatgaa gcacagggcc aggttggcgt tgcgttcgcg ggcggtgcgg
     1861 gccatgcggt cgagggcgag gtcgaagccg cgccggttgt agagcccggt gagggcgtcg
     1921 cgctcggcct ggtcggccag ctcggcggtc tggcgctggt gggtgatgtc ctcgtagatc
     1981 cagatgcggc cgacggaggt gccggtctcg tcgaggaccg gggaggtggt ctgggtgacg
     2041 atgcggtggt cgaggaggcg gaattcggcc aggtccgcgt ccgccagcgg gatggccatg
     2101 aagctgccgg tgtcgtcgag gaggcgctcg acgtaggggc gcaggcgcag ggcgggctga
     2161 tgcaccagcc cggcttcggg tgggatgcgc cagatctggc cgaaggtggc gttgtagtag
     2221 acgacgatgc cgtcgttgcc gacgaacagg atgccggtct tgagggcact cagcagggag
     2281 gccaggcgcg cccgttcatc gcgggcgtgt tcgtagtagc ggcgctgcag ggcctcgctg
     2341 aggaccagtt cctccacgcg gaccttgatg gtggcggcca tgcgcaggaa ggcggtgctg
     2401 agttggtcca cctcgtcgga gccgctgctc tccagctcca cgtcgtaatg gccgacggcc
     2461 agctggcggc tggcgtcggt gaggcgggcc aggcggcggg tgagccacag ggaaacccag
     2521 cccagggcga agagggccac cagcagcacg gcggctccca gttccaggct ttcgcggatc
     2581 aggcgctggc gttcgtcgtg caggaaacgc gtggacaacc cgaagcgcag ggtgccgagg
     2641 gcctggccgg cgagatcgat gtgcacctcg gtatgaaaga cccggtcggg acggtccaga
     2701 tcaaactgcg cggtcggatc gatggcgggg agcggtgtgc tggccgggcg tccctcgctt
     2761 gcgatgacct cgccgcggtg gtcgaggagg accaggtagg tgagcatttc gggggtgcgg
     2821 gcttcgtgca ggatctggcg cagggtcgcg tagtcccgct cggccagggg ggcgttgagg
     2881 gcggcgttga ggagggggcg ggtctgttcc agttgacggt tgagctggcc gagcaggctg
     2941 gcctcgatga ggcgtgcgct gtgggcgatg agcagggcga gggagatgct gcccaccacc
     3001 aggctggcga ccagcaggcg gaagcggagg gaggccggcc acagggcgtt catggtgcct
     3061 gcaggatctg gcgggcgtcc ggcagatgga tgtcgaggga cttcatgagg gcgtcgtcca
     3121 ccgtggtcag gccgctgaag ccggtcagct cggcgaagcg tcgcccgtcg ctgctgcccg
     3181 gaaagctctc caggcagcga cgcaggcgtt cgagggcgtc cgcgtccagg cgcggggagg
     3241 cgaggacgat gaagcccggc acctcggcga cggtctggac gaccttgagg cgggcacgga
     3301 tgtcttcggg gatcatgcgg aactcgccgc cgctgagcac ggctgcggtc gcgtcgccac
     3361 gcaggagcag gttgccgacg ctgtcgtcgg tgggcacgct gatcaggcgc aggtcctggc
     3421 ccggacgcag tccggcttcg gcgagccggt gcaggctctg cagggcgacg agggaatgga
     3481 cgttgggcag ggccacgatc ttgccgcgaa gatcggtcag gcggctcacg ggggcatcgc
     3541 cgcgggtgac caggagggcc gggatgtcgg gcgtgaagcg gcccacgggg atcaggccgg
     3601 cctcgacctg ctgcaggcgc gccagatggg cggcggtgac gatgaggtcg tagtcgcctt
     3661 ccagcccgcg acgggtgaag cgcgaccagt cgggcgccgt ggcgatgcgc gcggggcggt
     3721 tgaggctgcg cgccagggcg tcgcgcaggg gggcgtactg ggtcatgagc acgcgggtgc
     3781 tgagattggg cagcacgccg atttccagat cccgcatacc gtcggcggac gccacgcggg
     3841 cgcacagggc gagactgagg aggatgaggg cacggagcag ggctggcatc gaactggaga
     3901 accgctcggg acggtccggc gtgggctgga atggcaggtt accgcgattg ccgaacgtgc
     3961 tgctggcgtt ttacacaccg tttcgtgacg caggaaacag ggggccgtgg ccgattgcac
     4021 ggcgggcgat gcggcaggga taatccgtcg ctcctgcctt ccaccgcgag ccgtgtgccg
     4081 tcatgaccca gccttcaccc atcctgtccg ccctctaccg ctatcccgtc aagtccatgg
     4141 cgggggacgc cctgaacgcc agcgccgtgt cgcggctggg cttgcccttc gaccgcatgt
     4201 ggatggtggc cgatcgcagc gggcgcttcg tcaccgggcg ccagctgccc cagctggtgc
     4261 gggtgcgcac ggagccgtcg gccgacgggg tcacgctgac gatggcgggg cgcgaggcct
     4321 tgttcgtgcc aaacgaggtc ttcaccgagg aggtgtcggc ggtggtgtgg ggcagcctgt
     4381 tcccggcctg gggcggtgcg gcggcggcgg atgcctggct gtccgacgcc ctgggcgcgc
     4441 ccttgcgttt cttgtggacg ggcgcgcaga cggcgcggcg cgtgcgtgtc gagccggacg
     4501 tgcccctgtc cttcgcggac ggttttccgc tgctgctgat cggccagtcc tccctcgacg
     4561 atctgtcggc ccgggtcggc cggcctctgg agatggcgcg cttccgtccg aatctggtgg
     4621 tgtcgggggc ggaggccttt gcggaggatg gctggacgcg catccgcatc gggacggtga
     4681 gctttcgcct cgtcaaaccc tgcgagcgct gcgtgttcac gaccgtcgat cccgtgaccg
     4741 gcgagaagag cctggaccag gagccgctgc gcaccctggc ccgctaccgc aagactgcgg
     4801 atggcgtgct gttcgggcag aacgtgatcg cggacggggc cggcgagctg gtctgcggga
     4861 tgccggtgga cgtgctggaa tagcccgcgg gctggacttc tccgccaatt gacgctgcgg
     4921 caatccgtca aatgggtgtc gcaaaaaggc tgcacggtgc ccgttttggg ttgatgtaca
     4981 tcaagggctc gtcaattttg ggtagtgatt tcccctttga aattgtcccg ccgggtgtcg
     5041 ggtgatctaa tcggtccgac ccctacggag gaattcacgt gttcaaaaaa ttcaaaaatc
     5101 gtgtgcgggg cgcgctgggc ctgctgctgc tgggttttgc ggccctggcg atggccgacg
     5161 atgcgccccc gacgcccgag gagctggcga agatccggac cgccaacgcg gagtgtttca
     5221 agtgtcactc ggaagccggg ctccgcacgc cggtcaagga gggcatggat ctggccaagc
     5281 tgcgcacgct gctggtgaat ccggatacct atgcggcatc cgatcacggc gagatggcgt
     5341 gcagcaagtg tcacggcgac ggcttcgacg agcatcccca tgctcccaag gccaaggagg
     5401 acctggccga atgccaggac tgccacgccc gcaaggccat gcgcatcgag cgccagttcg
     5461 acaagtcggt ccatgcggag aacctcagcg accgcttcag ctgcgccacc tgccacaacg
     5521 cccacaccat gagcttcgcg gccaagctcc gcgatcccca caagatcgtg gcccaggaca
     5581 acaagatctg cctggactgc cacgacaacg acgaagcctt cgcaacgatg gcgccggaca
     5641 agaagaagcg cccggccatc gacgacatcc acaaatggct gcccaacacg cgcctgcact
     5701 ggcaggcggt gcgctgcatc gagtgccaca cccccaccga cgacaagctc tccctgtccc
     5761 acgaggtgca gaacaaggac aaggccgaga agaagtgcgt gacctgccac aacccggact
     5821 cgtccctcaa ggtgcggctc taccgccatc tggccaagga agagcagcag aaatacggct
     5881 tcctcaacag cgtgatcctg agtaattcct atgtggtggg ggcgacccgc aacccgaccc
     5941 tggatctgct gctcatcggc gccttcgtgg ccaccgtgct cggtgtgctg ggccacggtc
     6001 tgctgcgcat catcacgacc cgcatccgcc ggagcaagaa aaatgactaa gcgcgtctac
     6061 atgctcgccc cctggatcag gctgtggcac tggggcaatg ccctggcgat catcaccctg
     6121 tccattacgg gcatcagcct gcacttctcg ggctcggaac tggccctggt ggagttttcc
     6181 atggccgccc gcatccacaa cgtggccggc gtgtgcctgg cggtgctgta ctcggtcttc
     6241 gtgatcggca acatcgtcac cggcaactgg tggcagtacg tgcccaagcc gcccggcgtg
     6301 ctggggcgct gcctgaagca gatgcagttc tacggcagcg gcatcttcaa gggcgaagag
     6361 gagcctttcc cgccgacgcc tgaagtgaac ttcaatgccc ttcaggccgt gacctattgg
     6421 tcgatcatgt atctcgtcgt gccgacggtg atcgtgaccg gcctgatctt cctgtatccc
     6481 cagttcgcgc cggaccagat cttcggcatg gacggcctgc tgcccatcgc cctggtgcat
     6541 tacctggcgg cggcggtgat cctgctgttc atggtgagcc acatctacct gggcaccatg
     6601 ggcccgaagg tgagcagcct gttcaagatg atgatcaccg gctggtacga acactgaccc
     6661 cgtttcaccc aatcacaaca ttccgttctc aaggagcgcc ccgatgaaga tccgtaccgt
     6721 ctgtcttgcc accgccgccc tggcgaccgc gctgtccgcc ggcagcgccc tggctctgga
     6781 cgctgacgcc gcccaggccc tggccaagaa ggaaggctgt accaagtgcc acgccgtgga
     6841 caagaagaag gaagccaagt ccctcaccga gatctccaag tccctcaagg gcaaggccga
     6901 tgcggaagcc aagctgctgc atcacctcac gtccggcgag atggtcaagt tcgaggatgg
     6961 caaggaagag gagcacaaga tcctcaagac caaggacaag gccgagatca agaatctgac
     7021 cgactggatc ctgtcgctcg gcaagtagct cgcgtttgaa cgacccggtg tcgcgacgga
     7081 gcgcggcgcc ggtcatgcag cagtactagg gagatcatca tgaaaatttt ccgtaagttg
     7141 ttcgcgtcgt gtgccgtgtt cggcgcactg gtggccgccc aggcaagcct ggcggccgat
     7201 gcaccggcta cgccagccgc caagccggca gccaaggatc tggtgctcaa gggggacgcc
     7261 aagtgcacgt cctgccacga cgagacggat gaacccaaac tgctgcatat cggcaagacc
     7321 aagcacggct ccgtggccga cggccgcacg ccgagctgca ccaactgcca cggggaaagc
     7381 gatctccaca ccaacaaccc ggccaagctg aaggagcgtc cgcccacgga ccgcaacttc
     7441 ggcaagctgt ccaagctcag cgccgaggac aagagcgccg cgtgtaccac ctgtcaccag
     7501 ggcggcaagc acatcaactg gaacaccagc gcccacgcca accgggacgt gtcgtgcacc
     7561 tcgtgtcacc agatccattc gcccaaggac aaggtgcgcg acaagcgcca gcaggcggaa
     7621 gtctgctaca cctgccacaa gcagcagcgc gccgaggcca acaagccctc gcaccacccg
     7681 atccccgaag gcaagatgac ctgctcggac tgccacaatg cccacggttc ggccggtcag
     7741 aagatgctgg tgaaggacac caccaacgcc acctgcttca cctgccacgc cgagaagcgc
     7801 ggccccttcg tgcataacca ccagcccgtg gtggaggact gcggcaactg ccacaacccc
     7861 cacggcacca cggccgaagg catgctcaag tcccgtcagc ccttcctctg ccagcaatgc
     7921 cacggcgaca gctcccaccc cggcaacgtg cccggcgtgc gcagcctgat gcccaacgcg
     7981 gtcagcacca acatcggtcc gggcatggcc caggcgcgcg gctgcatgaa ctgccacacc
     8041 aacatccacg gcagcaacag ccccagcaat gcgacgagca gcggtccgac ccgcttcttc
     8101 cggtaatcga ggaggatgat catgagcacc cagcaacaat tccggcagac cgccctcgtt
     8161 atcgccctgt cggccgtgtt tcccctgagc agcgcatggg cggaggatga cgacgtcgcc
     8221 cagctgatca gccccaacgt gaccgaagtg acggtgaacg tgccgtgggt caacgaagtt
     8281 aaccccctgt atcgccagta caacgggatc aacaaccagg gcgtcaatgc caacctggac
     8341 ctgaacattg tgcgccgcag cgacgacgcc gagtggttcc gcctcaacgc ccgcaacctg
     8401 ggcctgagca cccaggaatt cggcgtgagc atggagaagc agggcgactg ggcccttggc
     8461 ctcaactacg accagatccc gcgctacgcg ccctacgcca tcagcacggc ggtgtccggc
     8521 atcggcacca acaccattac ccagccgagc tacgccaaca gcgcgttcac cagcgggtcg
     8581 ctgaccaacc cgaacctcta cgacgtcacc ctcaagaccg agcgggacat cgtgacgctc
     8641 aacgcgagca agttcgtggc caaggacgtg aagctctcct tcatgttcaa gaacgaggag
     8701 aagagcggcc tgcgcatgga cggtgtgcgc ggagtggcgg gcaccaatgg cagcacgggc
     8761 ggcactgcca accgctacag cggcttcctg ttctcgcccg agcccatcgg ccagacccac
     8821 aagcagttcg aggcgaccct ggacttcacg aacgagaagt accagatcac cgcgggtttc
     8881 tacggcagct tcctgaatac ccgggacaat tccctgaacg tgatcccggg aaccaacacc
     8941 cagctggtca ccaccacggc gccgctgctg tcgcccatcg ccctggcgcc ggacaactcc
     9001 atgcagcagt tcttcgtgag cggcgcctac aacttctcgc cggatacgcg ggcgaacctg
     9061 aaggtgtcgt attccgacgc gcgccagaac gacggcttct tctccggcca gccgaccatg
     9121 accggcatcg gcacgagtct gaacggagag gtgcgcacca ccgaagtgtt ctcgtcggtc
     9181 acctcgcgca tcaacaagaa cctcaagctg ctggcctcct ggcgctatga ggacaaggtg
     9241 gacaagacgc cgatccgtca gtacgtgccc acctcctttg gcgagcccta taacaatccg
     9301 gaatcccaca ccgccaactg gggcaagctg gaagccgact accgtctggg cggcggctac
     9361 agcgtgaccg ccggggtcga ctacaccaac aaggcgagcc tggaatggga gcgcaacaag
     9421 gtgtccgaga ccaccacgcg gctggccgtg cgcaaaacca tgagcgatgc gatcaacggc
     9481 accctgtccg tggcccattc agagcggacc ggttccgagt ggaccaccgg ctcagcaccg
     9541 ccgatctatc cggtctatct cgccgaccgc aagcgcaaca aggtccgcgg catggtggac
     9601 tggagcgcca ccgaggccct cagcctgcaa ctggcctatg aggcttactt cgacgactac
     9661 agcaagagcc tgtacggcct cgacaatggt cagggccagg tctattcggt ggatggcacc
     9721 taccagttca acgacacctg gaagctgaac gcctggtatt cccgccagaa cggcgagtcg
     9781 aaccagaatt cactcggtgc cgtgtgttcg tcgggcaaca atagcaactg cacgctgaac
     9841 acctacacca accgtaccgc gccgacggac ttcagccagt ggatcgactg gagcgccaag
     9901 atcaaaagca attccgatca ggtcggcttc ggtctgaagg gacgtatcca gaaggtggac
     9961 gtgggcatgg acttccttta cgcccgcgat cgcaaccagc aaaccctctc tggtctgccg
    10021 gcgacgacct ccacggcccg cagcggtact gcgcccaact acacctacac cccgaccgcc
    10081 ctggcggccg gcatgggtgt gctgccggac acgagctaca cccagaacac cgtgcggctc
    10141 aatggcacct atccgctcag caaggccacc aaggtgcgcc tggactacat ctacgacctg
    10201 cgcaagatgg acgactacac ctggagcaac tgggtgtatg cggacggcac caaggtctat
    10261 gtgaagcccg agcagaccac ccagatcatc ggcgtctccc tgagccacca gttctgaccg
    10321 gt
//
LOCUS       DAOQPA010000119        10309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3405_length_10309_cov_17.689975, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000119 DAOQPA010000000
VERSION     DAOQPA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_10309_cov_17.689975"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..621
                     /locus_tag="PKX01_14085"
     CDS             346..621
                     /locus_tag="PKX01_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77307.1"
                     /translation="MFEDDGMHTPADDELVVTPYALLRKGKDRKWDGWYDLARPNGAQ
                     VRTFVRVPSRDGFDDPHMACMAAEVLARFDLDGALKRTSSRRKPEVS"
     gene            646..918
                     /locus_tag="PKX01_14090"
     CDS             646..918
                     /locus_tag="PKX01_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HMZ77308.1"
                     /translation="MTPRLALTVAVLALFECSVPASAAVYKCDEGGRVVYMDRPCLYA
                     PVEAKSAVRTVGVPSGEGERRERVQPVRTLPADESAILQARPLVVR"
     gene            complement(1031..1690)
                     /locus_tag="PKX01_14095"
     CDS             complement(1031..1690)
                     /locus_tag="PKX01_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HMZ77309.1"
                     /translation="MKNLRTALIGSALALGTLFAVQGCIPVIVGGTGVAVAMATDRRT
                     SGTYVDDESIEWKASNRISEKLKDNVHANVTSFNRKVLLTGEAFNETSRDEAGRIAAG
                     VENVKEVVNELRIAPTSSLSARNNDAYISSKIKTRFLDRTDFHVQHVKVVTEAQTVYL
                     LGLVTRKEADAATQIARTTQGVNKVVRVFELISDEDAARLDSATGGSNENPVKSPNPR
                     S"
     gene            complement(1687..2280)
                     /locus_tag="PKX01_14100"
     CDS             complement(1687..2280)
                     /locus_tag="PKX01_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HMZ77310.1"
                     /translation="MDLVARIAQQFNDSAQTKLAALEFMAAPIAAAIETMTHCLMANG
                     KILACGNGGSAADAQHFAAELLNRFEMERPPLAAMALTTDTSTLTSIANDYAYDQVFS
                     KQVRALGQPGDVLLAISTSGNSPNVIEAIHAAREREMRVVALTGKGGGRMTGLLGPDD
                     VHLCVPADRTARIQEVHLLTLHCLCDGIDCLLLGVEE"
     gene            complement(2604..2987)
                     /locus_tag="PKX01_14105"
     CDS             complement(2604..2987)
                     /locus_tag="PKX01_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HMZ77311.1"
                     /translation="MRSRRYRARVAGLALVLCTGSSLAQQVRILVQNSPLAGFQYYSG
                     KILWDEMREGDPLVLVREPDNRHDTAAIRVEWRGVQLGHLPRAENAALAAEMDRGVKV
                     EGRIGRLTKDPNPWKRLRVEVYVRP"
     gene            complement(2977..3330)
                     /locus_tag="PKX01_14110"
     CDS             complement(2977..3330)
                     /locus_tag="PKX01_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMZ77312.1"
                     /translation="MQRGAAAEALAAQHLEGHGLRILARNVRCRGGEVDLIALDHDTV
                     VFVEVRLRRNGRFGGAAASITATKQQRVILAAQHWLAGAGRTHARRPCRFDAVLLDAL
                     SSAHIAWLKGAFDAE"
     gene            3447..4274
                     /gene="rsmI"
                     /locus_tag="PKX01_14115"
     CDS             3447..4274
                     /gene="rsmI"
                     /locus_tag="PKX01_14115"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMZ77313.1"
                     /translation="MYVVATPLGNLQDLGLRALAVLKAVDAVAAEDTRHSQRLLDAFG
                     IATKLVALHEHNEQSAVGQVIRLLEAGQHVALVTDAGTPAVSDPGARAVARVQEAGFP
                     VVPVPGPCAAVTALSASGLADGAFHFHGFLPVKSAARRAALEALRGVPGTLVFYEAPH
                     RIAECLDDLAAVLEAGRQIVVARELTKLFEQIVRMPLADAPAWLAADPNRARGEFVLL
                     VSAAPPAEGLSPEAERVLGLLLAELPVKQAAKLAAAITGASKNDLYERALALRDTAL"
     gene            complement(4287..5429)
                     /locus_tag="PKX01_14120"
     CDS             complement(4287..5429)
                     /locus_tag="PKX01_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77314.1"
                     /translation="MMDRWSGKWLVMLAVVLGIAGGAGTAGAETVVIGQCAPLTGSLG
                     STGKQMALGVRIAVAAANAAGGVGGHRLKHVVKDDGYQTSETVRLTRELIERDKAAAL
                     IGYAGTGNIVELLKQGVLADGTTALIAPYTGGEPLRSPYNPWIFHIRASYGDETEAMV
                     RQFVTTGIKRIAVFYQNDPFGQAGLAGVERALARFDLKVASSGSYEKNSEDVASAVQD
                     IAKGNPQAVIMIGVVRPAAAFVKAYRSVAPGTLLFSISVINGRELFQIAGGDFARGVG
                     ITQVMPSPFDGVSRIARDYADALRAYAPGEEPSYTSFEEYVGARVLIEAIRRVKGPPT
                     PLAVAQSLESLDLDVGGFKVKFGPGNRVGSRHVDVTLIGRDGSHIH"
     gene            complement(5551..6885)
                     /locus_tag="PKX01_14125"
     CDS             complement(5551..6885)
                     /locus_tag="PKX01_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ77315.1"
                     /translation="MSQPLRVLIIEDDPDVRLGCEQALMLADMAVDGVGSAEEGLARL
                     GPGFPGVVVTDMRLPRADGMEVLRRARALDADLPVIIITGHGDVTLAVEAMRGGAYDF
                     IQKPFSPEMLVDVVRRALDKRALTLEVLDLRRRLAERDGIDAKLIGRSPQMARVRQLV
                     ADVAGTRVDVLIRGETGTGKELVAQCLHELSPRKGGPFVALNCGGLPDTLLDSELFGH
                     EAGAFTGAQKRRIGKIEYANKGTLFLDEVESMPLGVQIKLLRVLQERVVERLGSNQQQ
                     PVDCRVVAATKDDLKARAEQHSFRADLYYRLNVVTIDLPPLRERREDIPLLLEHFMLG
                     AAQRHDRPLPSVPPERMRRLMAHPWPGNVRELRNMADCLVLGVGQERLDGAPDTVPPS
                     LAEAVDSFERSLIAAELRRQGGNLSRSAEALRIAKTTLHDKIRKHGLQGGDT"
     gene            complement(6882..8828)
                     /locus_tag="PKX01_14130"
     CDS             complement(6882..8828)
                     /locus_tag="PKX01_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77316.1"
                     /translation="MSPSHTPPPAEASTAEAPARSEAGTPPSRRVSTHPLLVAAFFIV
                     TGLIGLSAWRASEYQGIQTLRADSGHRVDLFAAAVDGIVNRYAHIPATIQLNEDVLQL
                     LRTPRHHPAVSAGNRFLQQLNSHVGSIAIFVLDERGIVLAASNWDHPDDSFVGEDLSF
                     RNYFLTALSGRTGHHFAIGITRGEPGYFVSHPIRDGDRVVGVAVIKISLEQLDRAWHL
                     LGVPALIADRNGVVILSSVPAWRYTALSPLPLDTRVEVRLSRLYNDAAIGAFPVTLPA
                     QLDPEGQIVETGAHLGEPGHRAGLTARYLVHGRDLPDLGWRLLIFSDLRPVQTQALGH
                     AALAAVATGFILLLLLVLAQRRRILRQKLEAKTLLEQANAQLESKVARRTRALTETNA
                     RLRKEVAERQQAEQTLRAAQDELVQAAKLAVLGQLAAGITHELAQPLGAMRTLSGNAV
                     AFMKRGDLDTVEKNLGIIARLADQMGRIITPLKTFARKSPAIPAATDLAHAVSAALFL
                     LDPRLAKAGVTVINRCEAGRDIAWCDQNRLEQVLINLIRNAADAMADAPVRELLIEAG
                     PDDAGGLALRIADSGCGLPDPAAGRLFEPFFTTKPAGEGLGLGLAISRDIVREFGGEL
                     SADNRPEGGARFTLHLPAAPKDAP"
     gene            complement(8946..10229)
                     /locus_tag="PKX01_14135"
     CDS             complement(8946..10229)
                     /locus_tag="PKX01_14135"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMZ77317.1"
                     /translation="MTTLSLPSYIDPAHTGPWGMFLEQLDRVAPHLGPLAEWLDTLRH
                     PKRCLVVDVPVRMDDGRIRHFEGFRVQHNLSRGPGKGGVRFHPDVTLSEVMALSAWMT
                     VKNAAVNLPFGGAKGGVRVDPKTLSTPELERLTRRYTSEIGTLIGPDRDIPAPDVNTN
                     EQIMAWMMDTYSINQGSTQTGVVTGKPVSMGGSLGRRDATGRGCFVVAREALKRLGRP
                     LTGARVAIQGFGNVGNAAARIFHDAGAKIVAIQDVAGTLHHPGGIDPHALLRFLDGDP
                     RRLTAYPEAGVIDNADFWSTDCEILLPAALEHQITADNAPRIRAKLIVEGANGPTTPQ
                     AEDILDAAGCTVLPDVLANAGGVTVSYFEWVQNASSFFWTEKDIHDRLDRILSEAFQA
                     IWELAAARRVNLRTATYIMACSRVLQARAQRGLYP"
BASE COUNT         1425 a         3270 c         3899 g         1715 t
ORIGIN      
        1 cgtttcgacc atttcctgcg gatctgctgc ggtgcggcct attcgccgtc catcgaggcg
       61 gcattgcgta cgctcgcccg gattgtctcg gccggctgac ccggcgcacc gggaccagcg
      121 ccctcgcgaa gcgccggcaa gatctgcaag ccgggcggcg tgtcgtcgcc tgtatgcctg
      181 ctgtatgaaa tccccatatt gagatttctt tgtaaatctt gattaaataa gtggttttct
      241 gtctctccga accctccgtc gtgcgttgtc gaaacacgca tgtctctata cttggccgcc
      301 tcgcgttccc cgcatcaggg gatgggcgga tcggaaatca ggtgcttgtt cgaggacgat
      361 ggaatgcaca ccccggcaga cgatgagctc gtggtgacgc cgtatgccct cctccgcaag
      421 gggaaggatc ggaagtggga tggttggtac gatctggcgc ggccaaacgg ggcgcaagtc
      481 cgtacctttg tccgcgtccc gtcccgtgat ggttttgacg atcctcacat ggcctgcatg
      541 gccgctgaag tcctggcccg cttcgatctc gacggggccc tgaaacgaac gtcctcccgg
      601 cgcaagccgg aggtttcctg acctgctgcc gccctggatg gcctcatgac cccgcgcctt
      661 gccctgactg ttgccgtact tgccctgttc gagtgttccg tacccgcgtc cgctgcggtg
      721 tacaagtgcg acgaaggggg gcgcgtcgtg tatatggacc ggccttgcct gtatgcgccg
      781 gtggaggcca agtccgccgt ccggacggtg ggcgtgccgt ccggcgaggg cgagcggcgc
      841 gaacgggtgc agccggtacg taccctgccc gcggacgaat cggccatcct gcaggcgcgt
      901 ccgctggtcg tccgctgaaa aggcggcggg gcgtgccgac gcaggcaagg ccacgcacgc
      961 accaacgcac caatgaaaac accccgcacc gggcggggtg ttgcgggact gtcggccgga
     1021 tcggcgccgg tcaggaacgg ggattcggtg acttgaccgg attctcgttg ctgccgccgg
     1081 tggcgctgtc gaggcgggcg gcgtcttcgt cgctgatcag ttcaaacacc cgcacgacct
     1141 tgttgacgcc ctgagtggtg cgggcgatct gggtggcggc atcggcttcc ttgcgggtga
     1201 ccagacccag caggtagacg gtctgggcct cggtgaccac cttgacgtgc tggacgtgga
     1261 agtcggtccg gtcgaggaag cgggtcttga tcttggagct gatgtaggcg tcgttgttgc
     1321 gcgccgacag gctgctggtc ggggcgatgc gcagctcgtt taccacctcc ttcacgtttt
     1381 cgacaccggc cgcgatgcgg cccgcttcgt cgcggctggt ctcgttgaag gcctcaccgg
     1441 tgagcagcac tttgcggttg aaggacgtga cgttggcgtg gacgttgtcc ttgagctttt
     1501 cggagatgcg gttggaggcc ttccattcga tggactcgtc gtccacgtag gtgccggaag
     1561 tgcgtcggtc ggtggccatg gcgactgcca cgccggtacc accgacgatc accgggatgc
     1621 agccctggac ggcgaaaagg gtgccgaggg cgagggccga cccgatcagg gcggtgcgca
     1681 ggttcttcat tcttcgactc cgagaagcag acaatcaata ccgtcgcaca gacagtgcag
     1741 ggtgagcagg tggacttcct gaatgcgcgc ggtgcggtcg gcgggcacgc acagatgcac
     1801 gtcgtccggg ccgagcagac cggtcatccg gccgccgccc ttgccggtga gggcgaccac
     1861 acgcatctcc cgttcgcggg cggcgtggat cgcctcgatg acgttgggcg aattgccgct
     1921 ggtggaaatc gccagcagca cgtcgccagg ctggccgagg gcgcgcacct gcttggagaa
     1981 gacctgatcg taggcgtagt cgttggcgat ggaggtgagc gtcgaggtgt cggtggtcag
     2041 ggccatcgcc gccagcggtg ggcgttccat ctcgaagcgg ttgaggagct ccgccgcgaa
     2101 gtgctgggcg tcggccgccg atccgccgtt gccgcaggca aggatcttgc cgttggccat
     2161 caggcagtgg gtcatggttt cgatggccgc ggcaatggga gcggccatga attcgagggc
     2221 ggccagcttg gtctgggcgc tgtcgttgaa ctgctgggcg atgcgggcga cgagatccat
     2281 ggggggctcc ggtagggtgc gcggattcta gcatcggggc gcggcgcttc gtggggagga
     2341 agaaatctgc ttacaaaggg cgtcgaggga gaggcgcggc ggtggcggcg cgggcaggcg
     2401 ccgtcgtgcc ggcgtgcccc gaggcgccgt ggcatctggg tgggcgagcg ggccgctgcg
     2461 agggtctgcc cggcctgggc tccgcgggcg tggcggcgtt cgccacgcgg ctttcagggg
     2521 cggtgcgtga ggcggctggc gccagaaggg cgcgggcctg tcgaggccgc ggaggcccgt
     2581 tcaaggtatc cggttggcgg ttgtcatggc cgcacataca cctccacgcg gaggcgcttc
     2641 cacggattcg gatccttcgt caggcgtccg atgcgacctt ccaccttgac gccccggtcc
     2701 atctcggcag cgagggcggc gttttcggcg cggggcaggt gaccgagctg gacgccgcgc
     2761 cattctacgc ggatcgcggc ggtgtcgtgg cggttgtcgg gctcgcggac gaggaccagc
     2821 gggtcgccct cgcgcatctc gtcccacagg atcttgccgc tgtaatactg gaagccggcc
     2881 aggggcgagt tctgcacgag aatgcgcacc tgctgggcga ggctgctgcc ggtgcagagg
     2941 acgagcgcga ggcctgcgac gcgcgcgcgg taccgtctac tccgcatcga aggcgccctt
     3001 gagccacgcg atgtgcgcgc tggagagggc gtccagcagc accgcgtcga agcggcaggg
     3061 ccgcctggcg tgggtgcgcc cggcgccggc cagccagtgc tgggcggcca ggatcacgcg
     3121 ctgctgcttg gtcgcggtga tgctcgccgc ggccccgccg aagcgcccat tgcggcgcag
     3181 gcggacttcg acgaacacca ccgtgtcgtg atccagggcg atcagatcca cctcgccgcc
     3241 gcggcagcgt acgttgcgcg ccaggatgcg caggccgtgg ccttccagat gctgggccgc
     3301 gagggcttcg gctgccgcgc cgcgctgcag gtgagcggct tgcgccggct cctcggcccc
     3361 gccacaatcg ggctcccccg cctttcgcca ccatgtccgc catgcttccc gcaccttccg
     3421 aatctcccct gtcaaggacg ccgtcattgt atgtggttgc cacgcccctc gggaacctcc
     3481 aggacttggg cctgcgggcg ctggcggtgc tgaaggctgt ggatgcggtg gcggccgagg
     3541 acacgcgcca cagccagcgc ctgctggatg cctttgggat cgccacgaag ctggtggcgc
     3601 tccacgagca caatgagcag tccgccgtcg ggcaggtgat tcggctgctg gaggccgggc
     3661 agcacgtggc cttggtcacc gacgccggca caccggccgt gtccgatccg ggcgcgcggg
     3721 cggtggcgcg ggtgcaggag gcgggcttcc cggtggtgcc ggtgccgggg ccctgcgctg
     3781 cggtgaccgc cctgtcggcc tccggcctgg cggacggggc cttccatttc cacggttttc
     3841 taccggtcaa gtcggcggcg cggcgggcgg ccctcgaggc gctgcgcggt gtccccggca
     3901 ccctggtgtt ctacgaggcg ccccatcgca tcgccgaatg tctggacgat ctggcggccg
     3961 tgctggaggc gggccgccag atcgtggtgg cgcgggaact gaccaagctc ttcgagcaga
     4021 tcgtgcgcat gccgctggcc gacgcgccgg cctggctggc ggcggatccg aaccgggcgc
     4081 ggggcgagtt cgtcctgctg gtctccgcag cgccgccggc ggaaggcctg tcgcccgagg
     4141 cggagcgggt gctgggcctg cttctggccg agctgccggt gaagcaggca gccaagctgg
     4201 ccgcggccat caccggggct tcgaagaatg acctgtacga acgggccctg gccctgcgtg
     4261 acacggccct gtgagttcgg cggccctcaa tggatgtgac tgccgtcgcg gccgatgagg
     4321 gtgacgtcca catggcgtga gccgacccgg ttgccggggc cgaacttcac cttgaagccg
     4381 cccacgtcca ggtcgaggct ttcgagggat tgggcgacgg ccagcggtgt gggcgggccc
     4441 ttgacgcggc ggatggcctc gatcagcacc cgtgcgccga cgtattcctc aaagctggta
     4501 taggacggct cctcgcccgg cgcataggcc cgcagggcgt cggcatagtc gcgggcgatc
     4561 cggctgacgc cgtcgaaggg cgagggcatg acctgggtga tgcccacacc ccgggcaaaa
     4621 tcgccgccgg cgatctggaa cagttcgcgc ccgttgatca ccgagatgct gaacagcagg
     4681 gtgccgggcg cgacactgcg gtaggccttg acgaaggcgg cggcggggcg caccacgccg
     4741 atcatgatga ccgcctgcgg gttgcccttg gcgatgtcct ggacggcact ggccacgtct
     4801 tcgctgttct tctcgtagct gccgctgctt gccaccttca ggtcgaacct ggccagggcc
     4861 cgctccacac cggccaggcc ggcctggccg aaggggtcgt tctggtagaa cacggcgatg
     4921 cgcttgatgc cggtggtgac gaactggcgc accatcgcct cggtttcgtc gccgtagctg
     4981 gcgcggatgt ggaagatcca cgggttgtag gggctgcgca ggggctcgcc gccggtgtag
     5041 ggggcgatca gcgcggtggt gccgtcggcg agcacgccct gcttgagcaa ttccacgatg
     5101 ttgccggtgc cggcgtagcc gatcagcgcg gcggccttgt cccgctcgat cagttcgcgg
     5161 gtcaggcgga cggtttcaga cgtctggtag ccgtcgtcct tgaccacgtg tttgagccgg
     5221 tggccgccca cgccgccggc cgcattggcc gccgccacgg cgatgcgcac gccgagcgcc
     5281 atctgcttgc cggtactgcc caggctcccc gtgaggggcg cgcactggcc gatcaccacc
     5341 gtttccgcac cggccgtgcc cgctccgccg gcgatcccca gcaccacggc cagcatgacc
     5401 agccattttc cgctccacct gtccatcatc gcgtcccccc cgcaacattg tgtcaatgtt
     5461 gcggaagtgt gcatgaatca tggtgcactg catatgacgc gtgccactgc cttcgagtga
     5521 actattacca gcccgcgggc agcgtgcgca tcaggtgtct ccgccctgca ggccgtgttt
     5581 gcggatcttg tcatggaggg tggtcttggc gatgcgcagg gcttcggcgc tgcggctcag
     5641 gttgccgccc tggcgacgca gttcggcggc gatgaggctg cgctcgaagc tgtccaccgc
     5701 ctcggccagg gacggtggga ccgtgtcggg cgcgccgtcg agccgctcct gaccgacgcc
     5761 cagaacgagg caatcggcca tgttgcgcag ctcgcgcacg ttgccgggcc aggggtgggc
     5821 catcaggcgg cgcatgcgtt cggggggcac gctgggcagg gggcggtcgt gacgctgggc
     5881 ggcgccgagc atgaagtgtt cgagcagcag ggggatgtcc tcgcgccgtt cccgcagggg
     5941 cggcaggtcg atggtcacca cgttgaggcg gtagtacagg tcggcgcgga agctgtgctg
     6001 ttcggcgcgg gccttgaggt cgtccttggt ggcggccacc acgcggcaat ccacgggctg
     6061 ctgctggttg gagccgagac gctcgaccac ccgttcctgg agcacgcgca ggagcttgat
     6121 ctgcacgccc aggggcatgc tctccacctc gtcgaggaag agggtgccct tgttggcgta
     6181 ttcgatcttg ccgatgcggc gcttctgggc gccggtgaag gcgccagcct cgtggccgaa
     6241 gagctcgctg tcgagcaggg tgtcgggcag gccgccgcag ttgagggcga cgaaggggcc
     6301 gcccttgcgc ggcgacagtt cgtgcaggca ctgggccacg agctccttgc cggtgccggt
     6361 ttcgccgcgg atcagcacgt ccacgcgggt gccggccacg tcggcgacga gctgacgcac
     6421 ccgcgccatc tgcggcgagc ggccgatgag cttggcgtcg atgccgtccc gctcggcgag
     6481 gcggcggcgc aggtccagca cctccagggt gagggcgcgc ttgtcgaggg cgcggcgcac
     6541 gacatctacc agcatctccg gggagaaggg cttctggatg aagtcgtagg ccccgccgcg
     6601 catggcctcc acggccaggg tgacgtcgcc gtggccggtg atgatgatga ccggcaggtc
     6661 cgcgtccagt gcgcgggcgc ggcgcagcac ctccatgccg tcggcgcggg gcaggcgcat
     6721 gtcggtgacg accacgccgg ggaagccggg acccaggcgg gcgagcccct cttccgccga
     6781 gccgacgccg tccacggcca tgtcggccag catcagggcc tgttcgcagc ccaggcgcac
     6841 gtccgggtcg tcttcgatga tgagtactct gaggggctgg ctcatggggc gtccttcggg
     6901 gccgcgggga gatggagggt gaagcgggcg ccgccttccg ggcggttgtc ggcgctcagc
     6961 tcgccgccga actcgcgcac gatgtcacgg gagatggcca gcccgagccc cagtccctca
     7021 cccgcgggct tggtggtgaa gaagggctcg aagaggcgcc cggcggccgg gtcgggcagg
     7081 ccgcagccgc tgtcggcgat gcgcagggcg aggccgccgg cgtcatccgg gccggcctcg
     7141 atgaggagtt cgcgcactgg cgcgtcggcc atggcgtcgg cggcgttgcg gatgaggttg
     7201 atgaggacct gctccaggcg gttctggtcg caccaggcga tgtcgcggcc ggcctcgcag
     7261 cggttgatga cggtgacgcc ggccttggcc aggcgcgggt cgaggaggaa cagggcggcg
     7321 ctcaccgcgt gggccaggtc ggtggcggcg ggaatggccg gcgacttgcg ggcgaaggtc
     7381 ttgaggggcg tgatgatgcg gcccatctgg tcggccaggc gggcgatgat gcccaggttc
     7441 ttctcgacgg tgtccaggtc accgcgcttc atgaaagcca cggcgttgcc cgacagggtg
     7501 cgcatggcgc ccaggggttg ggcgagttcg tgggtgatac cggcggcgag ctggcccagc
     7561 accgccagct tggcagcctg caccagctcg tcctgggcgg cgcgcagggt ctgttcggcc
     7621 tgctggcgtt cggccacttc cttgcgcagg cgggcgttgg tctcggtgag ggcgcgggtg
     7681 cggcgggcca ccttgctctc cagctgggcg ttggcctgct ccagcagggt cttggcctcc
     7741 agcttctggc gcaggatgcg gcggcgctgg gccaggacga gcaagagcag caggatgaag
     7801 ccggtggcca ctgcggcgag ggctgcatga cccagggcct gggtctgcac cgggcgcagg
     7861 tccgagaaga tcagcaggcg ccagcccagg tcggggagat cgcggccgtg gaccaggtag
     7921 cgggcggtga gtccggcccg atggccgggt tcgcccaggt gggcgccggt ctcgacgatc
     7981 tgcccctcgg ggtcgagctg ggcggggagc gtcaccggaa aggcgccgat ggccgcgtcg
     8041 ttgtagaggc gcgacagccg gacctcgacc cgcgtgtcca ggggcagcgg gctgagggcc
     8101 gtgtagcgcc aggccggcac cgagctcagg atcaccaccc cgttgcggtc ggcgatgagg
     8161 gccggcacgc ccagcagatg ccaggcccgg tcaagctgtt cgaggctgat cttgatgacc
     8221 gccacgccca ccacgcggtc accgtcgcgg atcgggtggg acacgaaata gcccggctcg
     8281 ccgcgggtga tgccgatggc gaagtggtgg ccggtgcggc cggagagggc ggtgaggaag
     8341 tagttgcgaa aggacaggtc ctccccgacg aagctgtcat cggggtggtc ccagttgctg
     8401 gcggcgagga caatgccacg ctcgtccagc acgaagatgg cgatgctgcc cacgtggctg
     8461 ttgagctgct gcaggaagcg gttgccggcg ctgaccgccg gatggtggcg gggcgtgcgc
     8521 agcagctgca ggacgtcctc gttgagctgg atcgtggccg ggatgtgggc gtagcggttg
     8581 acgatgccgt ccacggcggc ggcgaagaga tccacccggt ggccggagtc ggcgcgcagg
     8641 gtctggatgc cctgatattc gctggcccgc caggcgctca ggccgatgag gcccgtgacg
     8701 atgaagaagg ccgccaccag cagggggtgg gtcgacacgc ggcgggacgg cggcgtgccg
     8761 gcttcgctgc gggcaggcgc ttcggcagtg gacgcctccg ctgggggcgg cgtgtgggat
     8821 gggctcatgg acgggcggca atcgggcgcg gtgggcgctg cgggtggcgc tgcgtcgagc
     8881 atagcgcatc cgtaggcccg cagccgggcg ctgcgtcccg gccgggacgc aggccggcgc
     8941 ggcgctcagg gatacagccc ccgctgggca cgggcctgga gcacgcggga acaggccatg
     9001 atgtaggtgg cggtgcgcag attcacgcgg cgcgcggccg ccagctccca gatggcctgg
     9061 aaggcttccg acaggatgcg gtcgagccgg tcgtggatgt ccttctcggt ccagaagaag
     9121 ctcgacgcgt tctgcaccca ctcgaagtag ctcaccgtca ccccgccggc gttggccagc
     9181 acgtcgggca gcaccgtgca gccggccgcg tcgaggatgt cctcggcctg gggcgtggtc
     9241 gggccgttgg cgccttcgac gatgagcttt gcacggatgc ggggggcgtt gtcggcggtg
     9301 atctggtgtt cgagggcggc gggcagcagg atctcgcagt cggtgctcca gaagtcggcg
     9361 ttgtcgatga cgcccgcctc cgggtaggcg gtgaggcgcc gcgggtcgcc gtcgagaaag
     9421 cgcagcaggg cgtgggggtc gatgccgccc gggtggtgga gcgttccggc cacgtcctgg
     9481 atcgcgacga tcttcgcgcc ggcgtcatgg aagatccgtg ccgcggcgtt gcccacgttg
     9541 ccgaagccct ggatcgccac ccgcgcaccg gtgagggggc ggccgaggcg cttgagggct
     9601 tcccgcgcca ccacgaagca gccacgcccg gtggcgtcgc gtcgccccag gctgcccccc
     9661 atggagaccg gcttgccggt gacgacgccg gtctgggtgc tgccctggtt gatggagtag
     9721 gtgtccatca tccaggccat gatctgctcg ttggtgttca cgtccggcgc cggaatgtcc
     9781 cggtccgggc cgatgagggt gccgatctcg ctggtgtagc ggcgggtcag gcgctccagc
     9841 tccggcgtgg acagggtctt cggatcgacc cgcacaccgc ccttggcgcc gccgaagggc
     9901 aggttgaccg ccgcgttctt gacggtcatc caggctgaca gggccatgac ctctgacagg
     9961 gtcacgtccg ggtggaagcg cacgccgccc ttgcccggtc cgcgggacag gttgtgctgc
    10021 acgcggaagc cttcaaagtg gcggatgcgg ccgtcgtcca tgcggaccgg tacatccacc
    10081 accaggcagc gcttgggatg gcgcagggtg tcgagccact ccgccagcgg gcccaggtgg
    10141 ggcgccacac ggtcgagctg ctccaggaac atgccccacg ggccggtgtg ggccgggtcg
    10201 atgtaggagg gcagggaaag tgtcgtcatg aggggctccg cggttcagtg gttgaggatc
    10261 ttcgccagga actgctgggc ccgttccgac ctggggcgct cgaagaagg
//
LOCUS       DAOQPA010000120        10063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3535_length_10063_cov_20.103717, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000120 DAOQPA010000000
VERSION     DAOQPA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3535_length_10063_cov_20.103717"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..995
                     /locus_tag="PKX01_14140"
     CDS             69..995
                     /locus_tag="PKX01_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HMZ77318.1"
                     /translation="MPRIRILATGGTIAGASDPAGDGARYVAGVVDVDRLVGQVTGLS
                     ALAQVDAAQVLALDSKDMTPGHWQVLRPRICDALRDPGVDGVVILHGTDTLEETAWFL
                     HLTLPADKPVVLTGAMRPADHPQADGPANIVAAVSHAAAGAPGVRIVLNGGPWQAREL
                     VKARTLGLDAFSAGASTAPLRSAGCFAHLDNAALPRVDILPGYAGAPADLIDACVTAG
                     ARGLVLALAGHGSVPAAWVPALRRARNAGVRLLRASRCAGPVVHNGNADDDGEGYLTA
                     GDLPPLKARIALMLALADGMSFHATGERLATL"
     gene            complement(1032..2816)
                     /locus_tag="PKX01_14145"
     CDS             complement(1032..2816)
                     /locus_tag="PKX01_14145"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HMZ77319.1"
                     /translation="MQSLTTFAALLLATVLFVPWFKRAGLGTVLGYLAVGMLIGPSGI
                     KLVRDPDNLLHTAELGVVLLLFVIGLELKPSRLWALRHSVFGLGALQMAGVAAALAGV
                     GHYFGLSWSAASLVGIILALSSTAFVLPTLAERRELTTRYGRESFAILLFQDLSVIPL
                     LALIPLFGTGRPTDLTHPGVGLVLLVVIVLVGRRVLDALFAHVARVNSREMFTAAALV
                     TVLGLALIMQAAGLSMSLGAFVAGVLLADSEFRHELEAAIAPFQGLLLGLFFIAVGMS
                     TNLQLLVDQPGRIIGLTVGLVAMKAVVMYVLRRVLGGSKGDARMLALALAQGGEFAFV
                     LFDVAQAHRILGEVQADRLTLVVALSMAVSPLLLMLGDWLARLEAAREPARPFDDLPD
                     ESSEVVIAGFGRVGQIIGRLLVARGVRFTALDADADQIDTVRRFGFKVFYGDAANLDL
                     LHAAQVGSAKVFVLAIDNVEASLRTAELVRRHFPDVEVVARARNRFHANRLMDLGIQR
                     QIRETLPASLDMARWVLETLDDPPDLAAQLVARFARHDADLLARQQAISHDEKQLIQS
                     SRDARAELAQILEDARLAFAGKGKTRGS"
     gene            2839..3123
                     /locus_tag="PKX01_14150"
     CDS             2839..3123
                     /locus_tag="PKX01_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77320.1"
                     /translation="MAKVSHKRQPRRLQKRLKTRHGIDRHATSIFNVFVKFIYTKPLF
                     LKINFLDALRKPHQIAIFSKREHPGTNNANFCHTTPIVHVWLNKHPGDSP"
     gene            3120..4271
                     /locus_tag="PKX01_14155"
     CDS             3120..4271
                     /locus_tag="PKX01_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77321.1"
                     /translation="MKTHRLSAASASAILTACLAATAHAGPIAIYDGPAIDKTSDACA
                     SFGDAISCSLPYLNFLDGLAQGTTTSAGGYVLQSSQGLLKDSIVIQAGGNAALDNSDT
                     SPAPAVVENGFKSNSGSQHYLATGKTDGTTMSAGNMSDPTNNGLTASQDKIGTWDVSA
                     VWLRDALTTADGKRHELMIAFDYNQPQASATSMDIWSLITVRKSDGGLPDIEFEFNGD
                     TSGYASFSSTKNFYSQPSAADFDTVSGTICLYDNNQALPYPGGSCPKTYLGGSLVTEI
                     DNSQGTDRTEFLAFLPELNSNLENYIKQGYDTISVRLLMGCFGGTDPDNGQGYLSAGQ
                     TTNCDTGGFGDVYIMGGAAMNRIPEPSSLSLAVLALAGWMGRALPLRRT"
     gene            complement(4296..5237)
                     /gene="cysB"
                     /locus_tag="PKX01_14160"
     CDS             complement(4296..5237)
                     /gene="cysB"
                     /locus_tag="PKX01_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HMZ77322.1"
                     /translation="MNIQQLRYVFEVARHRLNVSEAAERLFTSQPGVSKQIRLLEDEL
                     GIEIFVRHGKRLVGITEPGRQVLSIAGRILQEVDNLREVGAEFSNEATGSLSIAFTHT
                     QARYALPRVVQAFMQRYPKVKLSFHQGNPRQVCEYVLSGEADIAIATEAIAEHEDIVM
                     LPCYQWNRCIIAPPRHPILSEQPLTLEAIARYPLVTYDFAFTGRNRINEAFTGRGLKP
                     NVVLTAIDSDVIKTYVAMGLGVGILARMAYDPAVDKDLVMMDASHLFASSTTRIGLRR
                     NAWLRGYVYAFIELFAPHLSRRVVDAALAGGGEDPGL"
     gene            5357..6136
                     /locus_tag="PKX01_14165"
     CDS             5357..6136
                     /locus_tag="PKX01_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ77323.1"
                     /translation="MSIAYTIAGFSVGAIVGLTGVGGGSLMTPLLVLLFGINPSVAVG
                     TDLLYAAITKAGGTLAHGLKGTVDWAVTRRLALGSIPASTVTLLLVGHFAPGGIGGAT
                     GLIKFSLGIALLLTAVAIVFRRQIQNFALAHSSGHEDPRRIALLTTLTGAVLGVLVSI
                     SSVGAGALGVSALFFLYPRLPTLRIIGSDIAHAVPLTLVAGIGHWFLGSVDWALLGSL
                     LIGSLPGIWLGSHVSTRVPDRVLRPILATMLVLVGSKLIVA"
     gene            6165..7847
                     /locus_tag="PKX01_14170"
     CDS             6165..7847
                     /locus_tag="PKX01_14170"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HMZ77324.1"
                     /translation="MYRYDSYDQRIVDERVAQFREQTRRYLAGELTDDEFRPLRLQNG
                     LYVQRHAPMLRVAVPYGLLSSAQVRTLAHIARTYDKGYAHFTTRQNVQFNWPDLKVVP
                     EILAELATVQMHAIQTSGACIRNITSDPFAGVAGDEHIDPRPWCEILRQWSTFHPEFA
                     FLPRKFKIAVNGAEEDRAVIQCHDIGLELKRDAAGQIGFRVLVGGGLGRTPIIGEEIS
                     AYVPWQHIITYCEAILRVYNRHGRRDNLYKARIKILVKALGIEEFRRQVEAEWLHTKD
                     GPSTLTEAEVARVAGHFTTPAYAALTAEDAVFAARRADTPAFAAWVRRNVRPHKVPGY
                     AIAILSLKHPKSPPGDITAEQLDLVASWADAYSFGELRATHEQNLVLADVKQSDLFTL
                     WEQARDAGLATPTFGLLTDIICCPGGDFCALANAKSIPIANALQARFDDLDYLHDIGE
                     LELNISGCMNSCGHHHVGHIGILGVDKNGEEWYQVSLGGSQGKRTSLGKVIGRSFAAA
                     EMPDVIGKILDVYVAQRQTAEAFIDTVRRIGLDPFKLGVYGEAAVKERKQHV"
     gene            7840..8364
                     /locus_tag="PKX01_14175"
     CDS             7840..8364
                     /locus_tag="PKX01_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HMZ77325.1"
                     /translation="MSKLIKNGRLSDDAAQILVLDETQAPEALTLPDGPVFVPLAVWQ
                     AHKGVLASRSDVGVWLDSHERVEELADDLGTLSAIALNFPKFTDGRAYSEAALLRSRY
                     GFTGEIRAIGDVLRDQFFYMQRCGFDAIQPKAGKYSDVQLEAALASLNDFSEPYQAAV
                     DHAAPLFRRAQRSA"
     gene            8378..9103
                     /locus_tag="PKX01_14180"
     CDS             8378..9103
                     /locus_tag="PKX01_14180"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HMZ77326.1"
                     /translation="MHPNQHPKLDDPALIAALDAKVATLVADLSAWAAELPAITMANS
                     LGAEDMVLTDVIATRGLPIEIFSLDTGRLPLETYDLMAEVQKRYGLKLKFYYPRHELV
                     EAYTRDHGINAFYDSVELRKACCHVRKVEPLGRALAGKQAWITGLRAQQSTTRTDLPK
                     RQFDEGNGLVKFNPLSDWSEKEVWAYIRIHQVPYNALHDKFYPSIGCAPCTRPISLGE
                     DIRAGRWWWENPETKECGLHVKK"
     gene            9139..>10063
                     /gene="cysD"
                     /locus_tag="PKX01_14185"
     CDS             9139..>10063
                     /gene="cysD"
                     /locus_tag="PKX01_14185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HMZ77327.1"
                     /translation="MSTLTQQTLSHLDWLESEAIHIMREVAGQCSNPVLLFSGGKDSI
                     CMLRIAEKAFRPGRFPFPLMHIDTGHNYREVVEFRDRRAAELGERLIVRSVEDSMKRG
                     TVVLKHENESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
                     DPKNQRPELWNLYNARSHKGENIRAFPISNWTEMDVWQYIEREELELPSIYFAHTRPV
                     VMRAGAIVPVNVPLMNGELTNLPKPGEEIIERQVRFRTVGDISCTAPVDSDADTVQKI
                     VLETATTTITERGATRLDDQTSEASMEQRKKE"
BASE COUNT         1865 a         3617 c         3006 g         1575 t
ORIGIN      
        1 ccttcttcga gcgccccagg tcggaacggg cccagcagtt cctggcgaag atcctgcagc
       61 actgagccat gccccgcatc cgcatcctcg ccaccggcgg caccattgcc ggtgcatcag
      121 accctgccgg cgacggcgcc cgttacgtgg cgggggttgt cgatgtggac cggcttgtcg
      181 ggcaggtcac aggtctgtcc gcgctggccc aggtggacgc ggcgcaggtg ctggccctcg
      241 acagcaagga catgacgcct ggccactggc aggtgctgcg gccccgcatc tgcgatgccc
      301 tgcgcgaccc cggggtcgac ggtgtggtga tcctccatgg caccgacacg ctcgaagaaa
      361 ccgcctggtt cctgcatctc accctgccgg cagacaagcc ggtggtactg accggcgcca
      421 tgcgccccgc tgaccatccc caggcggacg gacccgccaa catcgtcgcc gccgtgagcc
      481 atgcagccgc gggcgccccc ggcgtgcgga tcgtgctgaa cggcgggccg tggcaggcac
      541 gggagttggt caaggcgcgg acccttggcc tggacgcctt ttcagccgga gcctcgaccg
      601 cgcccctgcg cagcgccggg tgttttgccc atctggacaa cgctgccctg ccgcgggtgg
      661 acatcttgcc gggctacgcc ggcgcaccgg ccgacctgat cgacgcctgc gtgacggcgg
      721 gcgcccgcgg gctggtcctg gcccttgccg ggcatggcag cgtaccggca gcgtgggttc
      781 cggccctgcg ccgggcgcgc aatgcgggcg tccgcctcct gcgggccagt cgctgcgccg
      841 gcccggtcgt ccacaatggc aacgctgacg acgatgggga aggctacctc accgctggtg
      901 acctgccgcc gctcaaggcc cgcatcgccc tgatgctggc gctcgccgac ggcatgtcct
      961 tccacgcgac cggcgagcgc ctcgccaccc tgtaagcccg gcagacggtc cggccgtgac
     1021 gccggccgcg ttcagcttcc gcgggtcttg cccttgcccg caaatgcgag ccgggcgtcc
     1081 tcgagaatct gcgccagctc cgcacgcgca tcgcgagagc tctggatcag ctgcttctcg
     1141 tcatgggaga tggcttgctg gcgcgccagc agatcggcgt cgtggcgggc aaagcgggcc
     1201 accagctggg ccgcaagatc cggcggatcg tccagcgtct ccagcaccca gcgggccatg
     1261 tccagactgg ctggcaaggt ctcgcggatc tgtcgctgga tgcccaggtc catcaagcgg
     1321 ttggcatgaa aacggttgcg cgcccgggcc accacctcca cgtcgggaaa gtgacgacgc
     1381 accagctcgg ccgtacgcag ggacgcctcc acgttgtcga tggccagcac gaagaccttg
     1441 gcggacccca cctgcgccgc gtgaagcagg tcaaggttgg ccgcgtcgcc atagaacacc
     1501 ttgaagccga agcgccgcac cgtgtcaatc tgatccgcgt cggcgtccag ggcggtgaag
     1561 cgcacgccgc gcgccaccag caggcgaccg atgatctgtc cgacccgccc gaagcctgcg
     1621 atcaccacct cgctgctctc gtcgggcagg tcgtcaaaag gccgcgccgg ctcacgcgcc
     1681 gcctccagcc gcgcaagcca gtcgcccagc atcagcagca gcggtgaaac cgccatggac
     1741 aaggccacca cgagggtcag gcgatcggcc tgcacctcgc ccaggatgcg gtgcgcctgg
     1801 gccacgtcga acagcacgaa cgcgaattcc cccccctggg ccagcgccag ggcgagcatc
     1861 cgcgcgtccc ccttgctccc gcccagcaca cgccgcagga cgtacatcac caccgccttc
     1921 atggcgacca gcccgacggt cagtccgatg atgcgtccgg gctgatcgac cagcaactgc
     1981 aggttggtgg acatgcccac ggcgatgaag aacaagccca gcagcagccc ctggaacggc
     2041 gcgatggccg cctccagttc gtggcggaat tcggaatcgg cgagcagcac ccctgcaacg
     2101 aaggcaccca gggacatgga caggcccgcc gcctgcatga tcagggcgag gccgagcacc
     2161 gtcaccagcg ccgcggcggt gaacatctcg cgactgttca cccgcgccac gtgggcaaag
     2221 agcgcatcca acactctgcg cccgaccagc acgatcacca ccagcaggac gaggccgacg
     2281 ccggggtggg tcaggtcggt gggccggcca gtgccgaaca gggggatcaa ggccagcagg
     2341 ggaatcaccg acagatcctg gaacagcagg atcgcaaagg attcacggcc gtagcgggtg
     2401 gtcagctccc gccgctcggc cagggtcggg agcacgaagg ccgtggatga caaggccagg
     2461 atgatcccca ccagggaggc cgccgaccag gacagcccga agtagtgtcc gacccccgcc
     2521 agcgccgctg cgacgcccgc catctgcaag gcccccagcc cgaacacaga atggcgcagc
     2581 gcccacaggc gggacggctt cagctccaga ccaatgacga acagcagcag caccacaccc
     2641 agctcggctg tgtgcagcag attgtccgga tcccgcacga gcttgatgcc cgaaggaccg
     2701 atgagcatgc ccacggccag ataaccgagc accgtgccca gtccggcgcg cttgaaccag
     2761 ggcacgaaca ggacggtcgc cagcaggagg gccgcgaagg tggtcaggga ttgcatggga
     2821 cgaaaaggcg gggacgaagt ggccaaagtc tctcacaaac gacagccccg ccgcctgcaa
     2881 aagcgtctca aaacacgtca tggcatagac cgccacgcaa ccagcatttt taatgttttt
     2941 gtcaaattta tttacacaaa gccactgttc ctcaagatta attttcttga tgccctacga
     3001 aaaccgcatc aaatcgccat tttcagcaaa cgcgaacacc ctggcacaaa taatgcaaat
     3061 ttctgtcaca ccacgccgat tgtccatgtg tggctgaaca aacacccagg agactcgcca
     3121 tgaaaaccca tcgactgagc gccgcatccg cgtccgcaat cctgacggca tgcctggccg
     3181 ccaccgccca cgccggcccc atcgccatct acgatgggcc cgccatcgac aagacttctg
     3241 acgcctgcgc gagttttggc gatgccatca gttgctcgct gccctacctc aacttcctcg
     3301 atgggctggc tcaaggcacc accacctcgg cgggcggcta tgtgctccag tcctcccagg
     3361 gcctgctcaa ggactccatc gtgatccagg caggcggcaa tgccgcgctg gacaacagcg
     3421 acacctcgcc cgcaccggcc gtcgtggaga atggcttcaa gtccaattcg ggcagccagc
     3481 actacctggc caccggcaag accgacggca ccacgatgtc ggccggcaac atgtcggacc
     3541 cgaccaacaa cggtctcacc gccagccagg acaagatcgg aacctgggac gtgagcgccg
     3601 tctggctgcg cgacgcgctc accaccgccg acggcaagcg ccatgaactg atgatcgcct
     3661 tcgactacaa ccagccccag gcgtcggcga catccatgga catctggagc ctgatcaccg
     3721 tccgcaaatc cgatgggggt ctgcctgaca tcgagttcga gttcaatggc gacacctcgg
     3781 gctatgccag cttctcgagc accaagaact tctacagcca gcccagcgcc gccgacttcg
     3841 atacggtctc cggcacgatc tgcctgtacg acaacaacca ggccctgccc tacccgggcg
     3901 gttcgtgccc gaaaacctat ctgggcggca gtctggtcac cgagatcgac aattcccagg
     3961 ggaccgaccg gaccgagttc ctggccttcc tgccggagct caacagcaac ctcgagaact
     4021 acatcaagca aggctacgac accatttcgg tccgcttgct gatgggctgc ttcggcggaa
     4081 ccgatccgga caacgggcag ggctatctga gcgcgggcca gaccaccaac tgcgacacgg
     4141 gcgggtttgg tgacgtctac atcatgggcg gcgcggccat gaatcgcatc cccgaaccat
     4201 catccctgtc actggccgtg ctggcgctgg ccggctggat ggggcgcgcg ctgccgctac
     4261 gccgcacctg aacgggcccg ggatccaggc tgagctcaca ggcctggatc ctcgccccca
     4321 cccgccagcg ccgcatccac cacgcgccgg gacaggtgtg gcgcaaacag ctcgatgaag
     4381 gcgtacacat agccccgcag ccacgcattg cggcgcagac cgatacgcgt ggtgctggac
     4441 gcaaacaggt gtgaggcgtc catcatgacc aagtctttgt ccaccgccgg gtcgtaggcc
     4501 atgcgcgcca ggatgccgac gccgagcccc atggccacat aggtcttgat cacgtccgaa
     4561 tcgatggcgg tgagcaccac gttgggcttc agtccgcggc cggtgaaggc ctcgttgatg
     4621 cggttgcgcc cggtgaaggc gaaatcgtag gtcaccagcg ggtagcgggc gatcgcctcc
     4681 agcgtcagag gctgttcgga caggatggga tggcgcggcg gggcgatgat gcagcggttc
     4741 cactgatagc agggcagcat cacgatgtcc tcgtgctcgg cgatggcttc ggtggcgatg
     4801 gcgatgtccg cctcgccaga cagcacgtat tcgcacacct ggcgcggatt gccctggtga
     4861 aaggacagtt tgaccttcgg ataccgttgc atgaaggcct gcaccacccg cggcagggca
     4921 tagcgcgcct gggtatgggt aaaggcaatg gacaggctgc cggtggcctc gttgctgaat
     4981 tccgcgccga cctcgcgcag attgtccact tcctgcaaaa tgcggcccgc aatgctcaac
     5041 acctgccggc cgggttcggt gatgcccacc aggcgcttgc cgtgacgcac gaagatctcg
     5101 atgcccaact cgtcctccag caggcggatc tgcttggaca cgccgggctg ggaggtgaac
     5161 agacgctccg cggcttccga cacgttcagc cgatgccggg ccacctcgaa gacatagcgc
     5221 agttgctgga tattcatccg ggcctcctgt ccttcggaat acatataact tttttttcta
     5281 taagtctaac ccagaggtcc ttcttggctc tatcggttcc gtttacgatg cgccgcacaa
     5341 ggaatctgga atcatcatga gcattgccta caccatcgcg ggtttttctg ttggcgccat
     5401 tgtcggtctc accggcgtgg gcggcggctc gctgatgaca ccgctgctgg tgctgctgtt
     5461 cggcatcaat ccctcggtcg ccgtcggcac cgacctgctc tatgccgcca tcaccaaggc
     5521 cggcggcacc cttgcccatg gcctcaaggg cacggtggac tgggccgtca cacggcgcct
     5581 tgccctaggc agcattcctg cgtcgacggt caccctgctg ctcgtcggcc actttgcgcc
     5641 cggcggcatc ggcggcgcca ccgggctcat caaattctcc ctgggcattg ccctgctgct
     5701 caccgcggtc gccatcgtct tccgcaggca gatccagaac ttcgccctcg cccactcctc
     5761 gggccatgaa gacccccgcc ggatcgccct gctcaccacc ctgaccggtg cggtgctggg
     5821 tgtcctggtg tcgatttctt cggtgggcgc cggcgccctg ggcgtcagcg ccctgttctt
     5881 cctgtatcct cgcctgccga ccctgcgcat catcggcagc gacatcgccc acgccgtgcc
     5941 gctgacactc gtagccggca ttggccactg gtttctcggc agcgtggact gggccctcct
     6001 cggcagcctg ctgatcggct ccctgcccgg catctggctg gggagccacg tttccacccg
     6061 ggtgcccgac cgggtgctgc gccccatcct ggccaccatg ctggtattgg tcggcagcaa
     6121 actgatcgtc gcctgatccg gcggcgcccc ctccaggact aatcatgtac cgctacgact
     6181 cctacgatca gagaatcgtc gacgaacgcg tcgcccagtt ccgcgagcag acccgccgct
     6241 acctggcagg cgaactgacc gacgatgaat tccgccccct gcgtctgcag aacggcctgt
     6301 acgtgcagcg ccatgcgccc atgctgcggg tcgccgtccc ctacggcctg ctctcctcgg
     6361 cgcaagtgcg cacgctggcc cacatcgccc gcacctacga caagggctac gcccacttca
     6421 ccacgcgcca gaacgtgcag ttcaactggc cagacctgaa ggtggtgccc gagatcctcg
     6481 ccgagcttgc caccgtgcag atgcacgcga tccagacctc cggcgcgtgc atccgcaaca
     6541 tcacctccga ccccttcgcc ggcgtggcgg gcgatgagca catcgacccg cggccgtggt
     6601 gcgagatcct gcgtcagtgg agcaccttcc acccggaatt cgccttcctg ccgcgcaagt
     6661 tcaagatcgc cgtgaacggc gccgaggaag accgcgccgt catccagtgc cacgacatcg
     6721 ggctggaact gaaacgtgac gccgccggcc agatcggctt ccgcgtgctg gtgggcggtg
     6781 gcctcggacg caccccgatc atcggtgaag agatcagcgc ctacgtaccc tggcagcaca
     6841 tcatcaccta ctgcgaagcc atcctgcgcg tctacaaccg ccacgggcgc cgcgacaacc
     6901 tctacaaggc gcgcatcaag atcctcgtga aggcgctggg catcgaggaa ttccgccgcc
     6961 aggtcgaggc cgaatggctc cacacgaagg acggtccgag taccctcacc gaagcggaag
     7021 tggcgcgcgt cgccggccat ttcaccaccc cggcctatgc cgccctgacc gcggaagacg
     7081 ctgttttcgc cgcccgccgt gcggatacgc cggccttcgc agcgtgggtg cgccgcaacg
     7141 tgcggcccca caaggtgccg ggctacgcca tcgccatcct gtccctcaag catccgaaga
     7201 gcccgccggg cgacatcacc gccgagcagc tcgatctcgt cgccagttgg gccgacgcct
     7261 acagcttcgg cgaactgcgc gccacccacg agcagaatct ggtgctggcc gacgtgaagc
     7321 agtccgacct cttcaccctg tgggagcagg cccgcgacgc cggcctcgcc acgcccacct
     7381 tcggcctgct caccgacatc atctgctgcc ccggcggcga tttctgcgcc ctggccaacg
     7441 ccaagtccat ccccatcgcc aacgccctcc aggcccgatt cgacgacctg gactacctcc
     7501 acgacatcgg tgagctggag ctcaacatct ccggctgcat gaactcctgc ggccaccacc
     7561 acgtgggcca catcggcatc ctcggcgtcg acaagaacgg tgaggagtgg taccaggtca
     7621 gcctgggcgg cagccagggc aagcgcacca gcctcggcaa ggtgatcggc cgctccttcg
     7681 ccgctgccga gatgcccgac gtgatcggca agatcctcga cgtctacgta gctcagcgcc
     7741 agaccgccga agccttcatc gacaccgtgc gccgcatcgg cctcgatccc ttcaagctgg
     7801 gcgtctatgg cgaagccgcc gtcaaggaaa ggaagcagca tgtctaagct catcaagaac
     7861 ggccgtctga gcgacgacgc cgcccagatc ctggtcctcg acgagaccca ggctcccgaa
     7921 gccctgacgc tgccggacgg ccccgtcttt gtgcccctcg ccgtgtggca ggcccacaag
     7981 ggcgtcctcg cctcacggtc ggacgtgggc gtctggctcg acagccacga gcgggtggaa
     8041 gagctcgccg acgatttggg aacgctctcc gccatcgccc tcaactttcc caagttcacc
     8101 gacggacggg cctactcgga agccgccctg ctgcgcagcc gctatggctt caccggcgag
     8161 atccgcgcca tcggcgacgt gctgcgcgac cagttcttct acatgcagcg ctgcggcttc
     8221 gacgcaatcc agccgaaggc aggcaagtac tccgatgtcc agctcgaggc cgccctcgcc
     8281 agcctgaacg acttttccga accctaccag gccgccgtgg atcacgccgc gccgctgttc
     8341 cgccgcgccc agcgcagcgc ctgattcagg agcctccatg cacccgaacc agcatcccaa
     8401 gctcgacgat cccgccctga tcgccgccct cgacgccaag gtcgccaccc tggtggccga
     8461 cctgtctgcg tgggctgccg aactgccggc catcacgatg gccaacagcc tgggcgccga
     8521 ggacatggtc ctcaccgacg tgatcgccac ccgcggcctg cccatcgaaa tcttctccct
     8581 cgacaccggc cgcctgcctc tcgaaaccta cgacctgatg gccgaggttc agaagcgcta
     8641 cggcctcaag ctcaagttct actatccccg ccacgagctc gtcgaggcct acacccgcga
     8701 ccacggcatc aacgccttct acgacagcgt cgagctgcgc aaggcctgct gccacgtgcg
     8761 caaggtcgaa cccctcggtc gcgccctcgc cggcaagcag gcctggatca ccggcctgcg
     8821 cgcccagcaa tccaccaccc gcaccgacct gcccaagcgc cagttcgacg agggcaacgg
     8881 cctcgtcaag ttcaatcccc tgagcgactg gagcgagaag gaggtgtggg cctacatccg
     8941 catccatcag gtgccttaca acgccctgca cgacaagttc taccccagca tcggctgcgc
     9001 gccgtgcacg cgcccgattt ccctcggcga agacattcgc gccgggcgct ggtggtggga
     9061 aaatccggaa accaaggaat gcggcctgca cgtcaagaag tgaccctggc cgcctggaat
     9121 cattgaccga gtaaagcaat gtcgacgctc acccaacaaa ccctgagcca cctggactgg
     9181 ctcgaatccg aagccatcca catcatgcgc gaggtggccg gccagtgcag caatcccgtg
     9241 ctgctcttct ccggcggcaa ggactccatc tgcatgctgc gcatcgccga aaaggccttc
     9301 cggcccggcc gcttcccctt cccgctgatg cacatcgaca ccggccacaa ttaccgtgag
     9361 gtcgtcgaat tccgcgacag gcgcgccgcc gaactgggcg agcgtctcat cgtgcgctcg
     9421 gtggaagact ccatgaagcg cggcacggtc gtcctcaagc acgagaacga atcgcgcaac
     9481 aagcaccagt ccgtgacatt gctcgaagcc atcgaggagt tcggcttcga cgcctgcatc
     9541 ggtggcgccc gtcgggacga ggaaaaggcc cgtgccaagg aacgcatcat gagtttccgc
     9601 gacgagttcg gccaatggga cccgaagaac cagcgccccg agttgtggaa cctctacaac
     9661 gcccgcagcc acaagggcga gaacatccgc gccttcccca tctccaactg gaccgagatg
     9721 gacgtctggc agtacatcga gcgcgaggaa ctggaactgc ccagcatcta cttcgcccac
     9781 acccgccctg tggtgatgcg cgccggcgcc atcgtgccgg ttaacgtgcc gctgatgaac
     9841 ggtgaactga ccaacctgcc caagcccggt gaagagatca tcgaacgcca ggtgcgcttc
     9901 cgcaccgtgg gcgacatctc ctgtaccgcg ccggtggatt ccgatgccga cacggtccag
     9961 aagatcgtcc tcgagaccgc caccaccacc atcaccgagc gcggcgcgac ccgtctggac
    10021 gaccagacca gcgaagcctc catggaacaa cgcaagaaag agg
//
LOCUS       DAOQPA010000121        10049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3542_length_10049_cov_17.101561, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000121 DAOQPA010000000
VERSION     DAOQPA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_10049_cov_17.101561"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1237
                     /locus_tag="PKX01_14190"
     CDS             203..1237
                     /locus_tag="PKX01_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ77328.1"
                     /translation="MSLRIVPRLLAGLLTAALFATALPARAGKTLDGIRQKGQLACGV
                     STGVIGFSALDSQGRWQGLDVDVCKAIAAAVLGDAGKVRWVPLNSQQRFTALQSGEVD
                     LLSRNTTWSLTRDAGQGLHFAAITYYDGQGFLVPKKAKISSAKQLKGAEICVQSGTTS
                     EKNLADFFRAQGTKVKPVVFDRFDASLKAFFSGRCQAYTTDASSLAFIRMKEAPKPDD
                     YVVLPDLISKEPLGPLVRRGDDEWFAIVKWVVYALIEAEELGIGQARVDALKTSPDPA
                     IQRLVGSGDDLGKALGLDKDWSARALRAVGNYGEIFARNVGADSPLQLPRGLNAPWNK
                     GGLLYAPPLR"
     gene            1248..2204
                     /locus_tag="PKX01_14195"
     CDS             1248..2204
                     /locus_tag="PKX01_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ77329.1"
                     /translation="MAPRRVRLLVQALIVAACVAALAGLVDTTARHLAERGIRSGFDF
                     LLEPAGFSIGESPLPFAADDILLRAFAVGLLNTLRVALPGAALALCIGIVVGLLRLSA
                     HPLARGLGAAYVHAVRNLPLLLHLLAWYFVLTDLLPAADAPLHPLPGVFLSKSGLALP
                     WWGDDGLELPAPGDFGIDGGARLTPEYLALLAGLALYAAAYIAETLRAAVSALPAGQT
                     LAGLALGMRPRQTFFLVLLPQAWRAALPPVTSQLINLAKNASLGVAVGYPDLVSIAGT
                     TLNQTGRAVECLSIVIAVYLCLSLAGAALSRRLERAALRETT"
     gene            2201..>3248
                     /locus_tag="PKX01_14200"
     CDS             2201..>3248
                     /locus_tag="PKX01_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HMZ77330.1"
                     /translation="MIAGRLGRLRRALFGDPVATAISLATLAAVAFTLPPLIRWALLN
                     ARFAPDAAACDALDHAGACWGVVTEKYRLILLGRYPQDEAWRPVVASLLMGGGWLAGA
                     AGVLRGRTLAALLGLAPLAAAALLAGGVGGLAEVPAERWGGLPLTLLITATGLAGALP
                     LGIAIAYGRRARLPVLRGLLTAAVELARGIPLVAVLFMASFLLPLLLPEGTGPGMLGR
                     VLIAIVLVAAAYLSEIVRGGLQALPTGQRDAATALGLTPWQIERSIVLPQVLRAVAPS
                     LANSAIALFKDSALVTVVSLYELTGALGLALAGDPQWRAYQLEAYIFLAAIYWAGCAL
                     LARLAVRIGAPPEAG"
     assembly_gap    3249..3348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3349..4676)
                     /locus_tag="PKX01_14205"
     CDS             complement(<3349..4676)
                     /locus_tag="PKX01_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ77331.1"
                     /translation="MKRSILVVEDDPVLNHLLVKTLSRAGFDVSAARAWSEARLCLES
                     DAPELVLLDMNLPDARDFGPLAEVAQTRPVIMLTAFGSINQAVEAMRLGAVDYLVKPV
                     NLDELELVIHRALEGSRLRAGDALRTARSTGPAFSPMVGDSPAMRRLWELVQAVADDD
                     VTVMIRGESGAGKELVAQAIHAAGGRRDGHFVAVDCCTLQETLFESELFGHERGAFTG
                     ADRRKPGLIEAAAEGTLFLDEIGEIGPVVQAKLLRVLETGRFRRVGATTDLRANVRVV
                     LATNRDLARMVREGKFRADLYYRISAFEVTVPPLRERREDIAALVAHFARRRAGNGPV
                     PVFSPEALGALLAYDWPGNVRELRNVVERALILASRTGVVEPGHLPPLQGLGDDGADG
                     QIRIQGEPSLEEIERHYLADLLQRYDGNRRKVAEVLGVSERTAYRMLARYG"
     gene            complement(4673..6526)
                     /locus_tag="PKX01_14210"
     CDS             complement(4673..6526)
                     /locus_tag="PKX01_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77332.1"
                     /translation="MSLRAACSLSRRLTLVLMLALLMVSGMLSAGIWWVSREAMRREQ
                     EASAERIARLFEASLHTAMLRRDLGGLAEILSRLGHMQGVASAALLHPDGEIRFASRA
                     TDLGTRRPDLVAGLCLRSGCAESPPRIQPLEADDRLRIAYPIRNQARCAACHGPVDVH
                     PVNGVLVVEFKGLVEGDISGQMRGLLPLGLAALGLFALAVGWVVRREVLVPVARLGDV
                     ADRVAEGDLSARAGEPGVGELGRLGLRLDSMTERLGRMIEASGRQQAYLQSLLDAAPD
                     PMLVIAEDGCIVLANSAYLRLLGLAAGEAIGQPCHRVSRGLSEPCPTTLVRCPLAELR
                     ARPEPLRTVMSLRRRDGSEVSVEIHAAAVHTPDGRRMVVEAIRSLDDQVRFSQEQRLS
                     AVGLLANGVAHEIHNPLASIRLALQSSLRGLRDGTMSGEDVTEYLVLVDKEIDRCVAS
                     TQRLLTLSHPPGETRPVGVRAAVEDTLALLEAERQTTGVSVQICLEPPGARVMADEAE
                     LRQVILNLVQNAFHAMPRGGQLRVALGREGADYHLSVSDTGVGIGADDQALVFMPFYS
                     RRADGQQGSGLGLAICKAIVERHGGRIGVDSTPGVGSTFHVRLPAADGESP"
     gene            complement(6568..7575)
                     /locus_tag="PKX01_14215"
     CDS             complement(6568..7575)
                     /locus_tag="PKX01_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ77333.1"
                     /translation="MLNIDGDACVHARSATSRCTACVDACPRGAWTVDWREVAFQPDA
                     CDGCGVCVPACPAGVLELAQPPRLRPDGAGCACLVCERCADDGTLEADALRLPCVHGL
                     SEARILQAHGRGLRRLELRTGDCGTCPRGGGQPLTQRLAALNRALQARGAAPVVLAGT
                     EDAVRGVRPEDARRSFFRRFFERPGAALAEGAGGNAEGSDRRRDALERLARLGPGPGL
                     WSVVLDPARCNGCGVCATLCPELALGWLEGGGQAALRFDAARCTGCQLCVEVCDRDAL
                     RPAAVDEAWPGGPQHFFQVKPCRRCHAPIRRGATASEDADLCPICAVATVGRSGRLVV
                     D"
     gene            complement(7591..8244)
                     /locus_tag="PKX01_14220"
     CDS             complement(7591..8244)
                     /locus_tag="PKX01_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HMZ77334.1"
                     /translation="MSATDVLPVAAGIAVPAGLLCAAEDLAALAWFHEAERSPALIAA
                     LHEGGFPCGLGVLPADAPALLRFAAAVADVAGVRPDDAPGLADELAADFAAIYLTHAL
                     RASPCESVWRDEDHLMLQGSVFDVRARYQAHGVVARNWRLLPDDHLSNELAFVAHLLG
                     SAQADAAAHFLDAHLLVWLPDFAERVASRAATRFYATLADVTLAAVRAVRERLETGR"
     gene            complement(8241..8933)
                     /locus_tag="PKX01_14225"
     CDS             complement(8241..8933)
                     /locus_tag="PKX01_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ77335.1"
                     /translation="MADKKSIRKLSAEARAEVPAAQARRDAATYEPVKPARQLGFIHN
                     NMDCIGCRACEIACKDKNGLGPGPRFRRVMYVEGGQFPDVFAYKVNMSCNHCAEPACL
                     PTCPTGAIWKRADTGIVDIDSTLCIGCRRCEASCPYGAPQFDPVDNLVKKCNMCVDEI
                     DAGRKPYCVMACMMRVLDVGPIDQLRAGTYATKAMAPGEEPVRQVKHMASIELTNPSI
                     VFIPHPKGRVEE"
     gene            complement(8951..>10049)
                     /locus_tag="PKX01_14230"
     CDS             complement(8951..>10049)
                     /locus_tag="PKX01_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ77336.1"
                     /translation="GSDWFNQLTNINKEIQAIEKLDLVVCLDSTITPSGIWADVLFPV
                     ATHFERHDVALPWYKGHYYIHRPKVIQPLGESKSDFQIFTELAYRIEALDPTVKNFGK
                     RYNPRATRDYFRNPDAVDEAYLVAWWKGKVQAHQGVTMSWDEFKKRGVYKFTFKEPLV
                     AFRAQVEKGEPFPTGSGKIEIFSPYLAGITDWTLTQYGAPIPPIPRWIEPFESLNSPK
                     TKDFPFHLISPHPRWRTHSIFHNIPWLRETYDQEVTVNASDARRLGIQTGDTVEVWNE
                     RGTCVVPVYVTERCLPGVAVLFEGAWMDLDRDGVDRAGNPDFLTLDEPSPAGAFAYNT
                     VLVNIRKTELAHRPGWDQLGAARSTVFRRDY"
BASE COUNT         1451 a         3594 c         3436 g         1468 t
ORIGIN      
        1 caccttcaca cggtggaagc cactggttcg atcccagtac cgcccaccag attccgaaag
       61 ggccacgcca gcgcgtggcc cttttgcttt gcaggggctc ccggtccctc gccacactca
      121 cgggctttgc acggagcctt cgaagcccga taagctggcg ggcattccga ttctcccggc
      181 ggttcgatct gccgtccccg tgatgtccct acgcatcgtc ccccgcctgc tcgccggcct
      241 cctcaccgcc gcgcttttcg ccaccgccct tccggcccgg gccggcaaga cgctcgacgg
      301 gatccgccag aaaggtcagc tcgcctgcgg cgtcagcacc ggcgtgatcg gcttttccgc
      361 cctggacagc cagggccgct ggcagggcct ggacgtggac gtctgcaaag ccatcgccgc
      421 cgcggtgctg ggcgacgccg gcaaggtgcg ctgggtgccg ctcaacagcc agcagcgctt
      481 caccgccctg caatccggcg aggtggacct gctgtcccgc aacaccacct ggagcctcac
      541 ccgcgacgcg ggccagggcc tgcacttcgc agccatcacc tattacgacg gccagggctt
      601 cctggtgccc aaaaaggcga agatcagttc ggccaaacag ctcaagggcg ccgagatctg
      661 cgtccagtcc ggcaccacca gcgaaaagaa tctggccgat ttcttccgcg ctcagggcac
      721 caaggtcaag ccggtggtgt tcgaccgctt cgacgcgtcc ctcaaggcct tcttcagcgg
      781 gcgttgccag gcctacacca ccgacgcctc gtccctcgcc ttcatccgca tgaaagaggc
      841 gcccaagccg gacgactacg tggtgttgcc ggacctgatc tccaaggaac ccctcggccc
      901 gctggtacgc cgcggcgacg acgaatggtt cgccatcgtc aaatgggtcg tttacgccct
      961 catcgaagcc gaggagctgg gcatcggcca ggcccgcgtg gacgcgctga agacctcacc
     1021 cgacccggcc atccagcgtc tggtgggtag cggcgacgac ctgggcaagg ccctcggcct
     1081 ggacaaggac tggtcggccc gcgccctgcg cgccgtgggg aactacggcg agatcttcgc
     1141 ccgcaacgtg ggcgccgatt cgcccctcca gttgccccgc ggcctcaacg ccccgtggaa
     1201 caagggcggc ctgctctacg cgccgcccct gcgctgaagg ccatccgatg gcgccgcgca
     1261 gggtccggct gctggttcag gcgctgatcg tcgcggcctg cgtcgccgcc ctggcggggc
     1321 tggtggacac caccgcccgg catctggcgg agcgcggcat ccgcagcggc ttcgacttcc
     1381 tgctggagcc ggcaggcttt tccattggcg aatcgcctct gcccttcgcc gccgacgaca
     1441 tcctgctgcg agcctttgcg gtcggcctcc tcaacaccct gcgggtcgcc ctgcccggcg
     1501 ccgccctggc cctctgcatc ggcatcgtcg tcggcctgct gcgcctctcc gcccatccgc
     1561 tggcgcgggg gctgggtgca gcctacgtcc atgccgtgcg caatctgccc ctgttgctac
     1621 acctgctcgc ctggtatttc gtcctcaccg atctgttgcc cgccgccgac gcgcccctcc
     1681 atcccctgcc cggcgtgttc ctcagcaaga gcggcctggc cctgccctgg tggggtgacg
     1741 acggcctgga actgccggcg ccaggcgatt tcggcatcga cggcggcgcg cgcctcacgc
     1801 cggaatacct ggctctgctg gccgggctgg ccctctacgc cgccgcctac atcgccgaga
     1861 ccctgcgcgc cgcggtgagc gccctgcccg ccggccaaac cctcgccggc ctcgccctgg
     1921 ggatgcgccc gcgccagacc ttcttcctgg tcctgctgcc ccaggcctgg cgcgcggccc
     1981 tgccgccggt caccagccag ctcatcaacc tggccaagaa cgcgtccctg ggcgtggccg
     2041 tcggctatcc ggacctggtc tcgattgccg gcaccactct caaccagacc ggccgcgcgg
     2101 tggagtgcct gagcatcgtg attgccgtct acctgtgcct gtccctcgcc ggcgccgccc
     2161 tctcgcgccg cctggagcgg gccgccctgc gggagacgac gtgatcgccg ggcgcctcgg
     2221 ccggctacgc cgcgccctgt tcggcgatcc cgtcgccacg gccatctccc tggcgaccct
     2281 cgccgcggtc gccttcaccc tgccgcccct catccgctgg gcgctgctga atgcccgctt
     2341 tgcgccggat gcagcggcct gcgatgccct cgaccatgcc ggcgcctgct ggggcgtggt
     2401 gaccgaaaag taccgcctca tcctcctcgg ccgctatccc caggacgaag cctggcgccc
     2461 ggtcgtcgca agccttctca tgggcggcgg atggctcgcc ggcgccgccg gtgtgctgcg
     2521 cggccgcacc ctggccgccc tgctcggcct tgcaccgctg gcggcggcgg ccctgctggc
     2581 gggcggcgtt ggcggccttg ccgaggtgcc cgcagaacgc tggggcggcc tgcccctcac
     2641 cctgttgatc accgccaccg gactggccgg tgccctgccc ctcggcatcg ccatcgccta
     2701 cggccggcgc gcccgcctgc ccgtgctgcg gggcctgctg accgcagccg tcgagctggc
     2761 gcggggcatc ccgctggtgg ccgtgctgtt catggcgtcc ttcctgctgc ccctgctgct
     2821 gcccgaaggc accggaccgg gcatgctcgg gcgggtgctg atcgccatcg tcctggtggc
     2881 cgccgcctac ctgtcggaga tcgtgcgcgg tgggctgcaa gccctcccca ccgggcagcg
     2941 ggacgccgca acggccctcg gcctgacgcc ctggcagatc gaacgcagca tcgtcctgcc
     3001 ccaggtcctg cgcgccgtag cgccctccct cgcgaacagc gccatcgccc tcttcaagga
     3061 cagcgcgctg gtcacggtgg tgagtctcta tgaactcacc ggcgccctcg ggctggccct
     3121 ggccggagat ccccaatggc gggcctacca gctggaggcc tatatcttcc tggcggcgat
     3181 ctactgggcc ggctgcgcgc tgctggcgcg cctggcggtc cgtattgggg caccgcccga
     3241 agcgggtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gtagcgcgcc
     3361 agcatgcggt aggcggtgcg ctcggagacg cccagcacct ccgccacctt gcggcgattg
     3421 ccgtcgtagc gctggagcag atccgccagg taatgacgtt cgatctcctc caggctgggc
     3481 tcgccctgga tgcggatctg cccgtccgca ccgtcgtccc ccagaccctg caggggcggc
     3541 aggtgacccg gttcgacgac gccggtacgg ctggcgagga tgagggcccg ctccaccacg
     3601 ttgcgcagct cacgcacatt gccgggccag tcgtaggcca gcagggcccc cagcgcctcg
     3661 ggcgagaaca ccggcaccgg cccgttgccg gcacgccgcc gggcgaaatg ggcgaccagc
     3721 gcggcaatgt cctcgcgccg ctcgcgcagg ggcggcacgg tgacctcgaa ggcgctgatg
     3781 cggtagtaga gatccgcgcg gaacttgcct tcgcgcacca tgcgcgccag atcccggttg
     3841 gtggcgagca ccacccgcac gttggcgcgc aggtcggtgg tggcgcccac ccgccggaag
     3901 cgcccggtct cgagcacgcg caggagcttg gcctgcacga cggggccgat ctcgccgatc
     3961 tcgtcgagga acagggtgcc ctccgcagcg gcctcgatga ggcccggctt gcgccggtcg
     4021 gcgccggtga aggcgccccg ctcgtggccg aagagctctg actcgaacag cgtctcctgc
     4081 agggtgcagc aatccaccgc gacgaaatgc ccgtcgcgcc gcccgccggc cgcgtggatc
     4141 gcctgggcca ccagctcctt gccggcaccg ctctcgccac ggatcatcac cgtcacatcg
     4201 tcgtccgcaa cggcctggac gagctcccac agccgacgca tggccgggct gtcgcccacc
     4261 atcgggctga acgccgggcc ggtgctgcgg gccgtgcgca gggcatcgcc ggcgcgcagg
     4321 cgggagcctt ccagggcgcg gtggatgacc agctccagct cgtccagatt gaccggcttg
     4381 accaggtaat ccactgcgcc caggcgcatg gcctccacgg cctgattgat ggaaccgaag
     4441 gccgtgagca tgatcaccgg gcgggtctgg gcgacctccg ccagcgggcc gaagtcccgc
     4501 gcatcgggca gattcatgtc cagcagcacc agctccggcg cgtcgctctc gaggcaaagg
     4561 cgggcctcgg accaggcgcg cgcggcggac acgtcgaaac ccgcacggga cagggtcttg
     4621 acgagcaggt ggttgaggac ggggtcgtct tcgaccacca ggatggagcg cttcatgggg
     4681 attctccgtc ggcggcgggc aggcgcacat ggaaggtgct gccgacgccc ggcgtgctat
     4741 caacgccgat acggccaccg tgccgttcga cgatggcctt gcagatggcg agaccgagcc
     4801 cggagccctg ctggccgtcg gcccggcgac tgtagaaggg catgaacacc agggcctggt
     4861 catcggcacc gatgcccaca ccggtatcgc tcacgctcag gtgatagtcg gcaccttccc
     4921 gacccagggc gacccgcagc tgaccgccgc ggggcatggc gtggaaggcg ttctggacca
     4981 gattgaggat cacctggcgc agctcggcct catcggccat cacgcgcgcc ccggggggtt
     5041 cgaggcagat ctgcacgctc acgccggttg tctggcgttc ggcttcgagc agggccaggg
     5101 tgtcctccac ggcggcacgc acgcccaccg gacgcgtctc gccgggcggg tggctgaggg
     5161 tcaggaggcg ctgggtcgag gcgacgcagc ggtcgatctc cttgtccacc agaaccaggt
     5221 attcggtgac gtcctcgccg ctcatcgtgc cgtcccgcag gccgcgcagg ctggactgga
     5281 gcgccagacg gatggacgcc agcgggttgt ggatctcgtg ggcgacgccg ttggcgagca
     5341 gccccacggc cgacaggcgc tgctcctggg agaagcgcac ctgatcgtca agggagcgga
     5401 tcgcctcgac gaccatgcgg cggccgtccg gggtgtgcac ggcggccgcg tggatctcga
     5461 cgctcacctc gctgccgtca cgccgacgca ggctcatcac cgtgcgcagg ggctccggcc
     5521 gcgcccgcag ttcggccagc ggacagcgca ccagggtggt cggacagggt tcggacagac
     5581 cgcggctgac ccggtggcag ggctggccga tcgcctcgcc ggccgcgagc ccgagcagac
     5641 gcaggtaggc gctgttggcc agcacgatgc acccgtcttc ggcgatgacc agcatgggat
     5701 cgggcgccgc gtccagcaga ctctgcaggt aggcctgctg acggcccgag gcttcgatca
     5761 tgcggcccag ccgctcggtc atggaatcga ggcgcagccc caggcggccc agctcgccca
     5821 cgcccggttc gccggcgcgg gcactcagat cgccctccgc gacccggtcg gctacgtccc
     5881 ccaggcgcgc caccggcacc agcacctcgc gccgcacgac ccagcccacc gccagggcga
     5941 acagcccgag ggcggccaga ccgaggggca gaagccccct catctggccg gagatgtcgc
     6001 cttccacaag gcccttgaac tccaccacca gcaccccgtt cacgggatgc acgtccacgg
     6061 gcccgtggca ggcggcgcag cgggcctggt tacggatcgg gtaggcaatg cgcaggcggt
     6121 catccgcctc cagcggttgg atacgcggcg gcgattcggc acagccgctg cgcaggcaca
     6181 ggccggccac cagatcgggc cgccgggtgc ccaggtcggt agcccgggac gcgaaccgga
     6241 tctcgccgtc aggatggagc agcgccgccg aggccacccc ctgcatatgc cccaggcgcg
     6301 agaggatctc ggccaggccg cccagatcgc ggcgcagcat ggccgtgtgc aggcttgctt
     6361 cgaagagccg cgcaatgcgc tcggccgagg cctcctgctc gcgccgcatc gcctcccgcg
     6421 acacccacca gatcccggcc gacagcatgc cggacaccat cagcagggca agcatgagga
     6481 ccagggtgag gcgacgggac agggagcagg cggcgcggag ggacacggga cggatgggcg
     6541 ttggatgggg gtcggatgac cgaattttca gtctaccacc aggcgtccgg agcgccccac
     6601 ggtggccacg gcgcagatcg ggcacaggtc ggcatcctcg gacgcagtgg caccgcgccg
     6661 gatcggcgcg tgacaccgtc ggcacggctt gacctggaag aagtgctgcg gcccgccggg
     6721 ccacgcctcg tccaccgcag ccggacgcag cgcatcgcgg tcgcacactt cgacgcacag
     6781 ctggcagccg gtacagcgcg cggcgtcgaa acgcaatgcc gcctgcccgc caccctccag
     6841 ccagccaagg gccagctccg ggcacagggt cgcgcacacg ccgcagccgt tgcagcgggc
     6901 ggggtcgagg accacgctcc acagcccggg accgggaccc agccgggcca ggcgctcgag
     6961 ggcgtcgcgg cgccggtccg agccctcggc gttgccaccg gcgccctccg ccagggctgc
     7021 gccgggacgt tcgaagaagc ggcggaaaaa actgcggcgg gcgtcttcgg gacgaacgcc
     7081 gcgcaccgcg tcttccgtcc cggccagcac gaccggcgcc gcgccgcgag cttgcagggc
     7141 ccggttgagc gccgcgagac gctgtgtgag gggctgcccc ccgccgcgcg gacaggtgcc
     7201 acagtcaccg gtccgcagtt cgagccggcg aagtccccgc ccgtgggcct gcaggatgcg
     7261 ggcctcgctg agcccatgga cgcagggcag cctgagcgca tccgcctcaa gcgttccgtc
     7321 gtccgcacag cgctcgcaga cgaggcacgc acaccccgcg ccatcgggcc ggagcctggg
     7381 cggctgcgcc agttccagca cgcccgcagg acaggccggc acgcacacgc cacagccgtc
     7441 gcaggcgtcg ggctgaaagg cgacctcccg ccagtccacg gtccacgcac cgcgcggaca
     7501 ggcatcgaca cacgcggtac accgggaggt ggccgagcgg gcgtggacgc aggcgtcgcc
     7561 gtcgatgttc agcatgacct cggccgcgcc tcagcgaccg gtctcgagac gctcgcggac
     7621 cgcgcggacc gccgccaggg tcacgtccgc cagcgtggca tagaagcggg tcgcggctcg
     7681 gctggccacc cgctcggcga aatcgggcag ccagaccagc aggtgcgcgt cgagaaagtg
     7741 cgccgccgcg tctgcctgag cgctgcccag cagatgggcg acgaaggcca gctcattgct
     7801 gaggtgatcg tccggcagca gccgccagtt gcgggccacg acgccgtggg cctggtagcg
     7861 ggcgcgcacg tcgaagacgc tgccctggag catcaggtgg tcctcgtccc gccacacgga
     7921 ttcgcagggc gaggcgcgca gggcgtgggt aaggtagatg gcggcgaagt cggccgccag
     7981 ctcgtcggcc aatcccggcg catcgtccgg gcggacgccg gcaacgtcgg ccacagccgc
     8041 ggcaaagcgc aggagcgccg gcgcgtcggc cgggagcacg cccagtccgc agggaaagcc
     8101 gccctcgtgc agggcggcga tcagcgccgg cgagcgctcg gcctcgtgga accaggccag
     8161 ggccgccaga tcctcggcgg cgcagagcag gccggccggc acggcgattc cggccgccac
     8221 gggcagcacg tccgtggcac tcattcttcg acccgtccct tgggatgggg aatgaagacg
     8281 atggacggat tggtcagctc gatgctggcc atgtgcttga cctgacgcac cggctcctcg
     8341 cccggcgcca tggccttggt ggcgtaggtg ccggcgcgca gctggtcgat gggccccacg
     8401 tcgagcacgc gcatcatgca ggccatgacg cagtagggct tgcgcccggc gtcgatctca
     8461 tccacgcaca tattgcactt tttgaccagg ttatccaccg ggtcgaactg gggcgcaccg
     8521 taggggcagg aggcctcgca gcggcgacag ccgatgcaca gggtggagtc gatgtccacg
     8581 atgccggtat cggcgcgctt ccagatggcc ccggtgggac aggtgggcag gcaggccggt
     8641 tcggcgcagt ggttgcagga catgttcacc ttgtaggcga agacatccgg gaactggccg
     8701 ccttccacgt acatcacgcg gcggaagcgc ggcccggggc cgaggccgtt cttgtccttg
     8761 caggcgatct cgcaggcccg gcagccgatg cagtccatgt tgttgtggat gaaaccgagc
     8821 tgcctggcgg gcttgaccgg ctcgtaggtg gccgcgtcgc ggcgcgcctg ggcggccggc
     8881 acctcggcgc gggcctcggc ggagagcttg cggatgcttt tcttgtcagc catggaatgc
     8941 gcccccttgc tcagtagtca cgccggaaca ccgtggaccg cgctgcgccc agctggtccc
     9001 agccgggacg atgggccagt tcggtcttgc ggatgttcac cagaacggtg ttgtaggcga
     9061 aggcaccggc cgggctcggc tcgtccaggg tgaggaagtc cgggttaccg gcccggtcga
     9121 cgccgtcccg gtccaggtcc atccaggccc cctcgaagag caccgcgaca cccggcaggc
     9181 agcgttcggt gacgtacacc ggcaccacgc aggtgccccg ctcgttccac acctccaccg
     9241 tgtcgccggt ctggatgccg agacgccggg cgtcggaggc gttgacggtc acttcctggt
     9301 cgtaggtttc ccgcagccac gggatgttgt ggaagatgga gtgggtgcgc cagcgcgggt
     9361 gcgggctgat caggtggaag gggaagtcct tggtcttcgg gctgttgagg gactcgaaag
     9421 gctcgatcca gcggggaatc ggcggaatcg gcgcgccgta ctgggtgagc gtccagtcgg
     9481 tgatgccggc caggtagggc gagaagatct cgatcttgcc cgatccggtg gggaacggtt
     9541 cgcccttctc cacctgggca cggaaggcca ccagcggctc cttgaaggtg aacttgtaga
     9601 cgccacgctt cttgaattcg tcccagctca tggtgacgcc ctggtgcgcc tgcaccttgc
     9661 ccttccacca ggcgacgagg taggcctcat ccactgcgtc ggggttgcgg aagtagtcgc
     9721 gggtggcacg cgggttgtag cgcttgccga agttcttgac ggtgggatcc agcgcttcga
     9781 tccgataggc cagctcggtg aagatctgga agtcggactt gctctcgccc aggggctgga
     9841 tcaccttggg gcggtggatg tagtagtggc ccttgtacca gggcagcgcc acgtcgtgcc
     9901 gctcgaagtg ggtggccacc gggaagagca cgtcggccca gatgcctgaa ggggtgatgg
     9961 tggaatccag gcacaccacg agatccagct tctcgatggc ctggatctcc ttgttgatgt
    10021 tggtgagctg gttgaaccag tcggagccc
//
LOCUS       DAOQPA010000122         9753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3704_length_9753_cov_18.254382, whole genome shotgun sequence.
ACCESSION   DAOQPA010000122 DAOQPA010000000
VERSION     DAOQPA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3704_length_9753_cov_18.254382"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKX01_14235"
     CDS             complement(<1..411)
                     /locus_tag="PKX01_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77337.1"
                     /translation="MLKRFFSARHTGWIAAAHQGDALDLVHVSRDGSGRPQLHQAVRQ
                     AGVEGDLAARIASATRASGLRKGRHTWLLHGLDYQILQVDTPDVPEAEQRNALRWQLK
                     DALQQRVEDSVFDLLPLPPPAHARGRPPALVIAAA"
     gene            complement(568..993)
                     /locus_tag="PKX01_14240"
     CDS             complement(568..993)
                     /locus_tag="PKX01_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77338.1"
                     /translation="MKPRLHARGMALPAAIFLLVIMAALGAFLMRIASLQQAGSAQDI
                     LSSRAFQAARAGVEWALYQAQRNGSCSAATGFSPGGNLADFTVSVTVSTTSYTEAGTA
                     LRLCAVTATACNQPVAGACPGSAGTRYYVERQLRASFPY"
     gene            complement(990..1808)
                     /locus_tag="PKX01_14245"
     CDS             complement(990..1808)
                     /locus_tag="PKX01_14245"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ77339.1"
                     /translation="MHPTRPPEQRSPREAGFTLIEAIVTMTVLAIVGGMIAVFLRTPI
                     DAYTDTARRADLTDTADTALRRFDREIRTALPNSIRSAASGGVLYLEFLQTTGAGRYR
                     IAATSPGVGNPLDFTAADTSFDILGSIPSVPGGAFLVVMNLGAGSGADAYAGDNRSTI
                     ASLSGSTLSFASFLFPFDSPAHRFFVVDTPVTYACNPAAGTLTRISGYTIAASQPTPP
                     ASGTVTLVADKVSACNFTYDAIASRTGMVSASLTLTSGGESVRLFRQVAVSNAP"
     gene            complement(1786..2334)
                     /locus_tag="PKX01_14250"
     CDS             complement(1786..2334)
                     /locus_tag="PKX01_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ77340.1"
                     /translation="MYTSAAGPAPLRGVTLIELIVFIAIVSVGLAGLMSTYTAAVRGS
                     ADPMQRKQALAIAEALLEEVSRAAFTWCDPADANLDTATSAAGCASLPENSGPEAGNS
                     RPYDNVNDYAGFTLAGITDQSGNAISGLSGYSANVAVSAAALNGIAAGEALRVTVTVT
                     APDGNSYALDAWRTRYAPNAPP"
     gene            complement(2324..2791)
                     /locus_tag="PKX01_14255"
     CDS             complement(2324..2791)
                     /locus_tag="PKX01_14255"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ77341.1"
                     /translation="MRKSPFAGATAGFTLIELIAVLLLTGILAIAVLPRMADRSAFDS
                     RGFTDTTRATLQHARRVAIAQRRTVCGTYSGGTLAFTQSSTNGAAGCDLPLPDPAGNA
                     ALSLTPPSGVSLSANPTSFTFDGLGRPNAAVTLTIVGDITRTLTIEADSGYVH"
     gene            complement(2814..3221)
                     /locus_tag="PKX01_14260"
     CDS             complement(2814..3221)
                     /locus_tag="PKX01_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ77342.1"
                     /translation="MKQQSGFTLIELVVVIVILGILAAVAVPKFVDLSTDAGDAAAAG
                     VAGAITSASSLNYATSVARGASGTGVLQVKSGGVCSTLAGSLLTNGVPTNITIAGGPL
                     TCSGAGVTDTSCTVAHAKGTAGKTFAVTMTCTQ"
     gene            complement(3333..3836)
                     /locus_tag="PKX01_14265"
     CDS             complement(3333..3836)
                     /locus_tag="PKX01_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77343.1"
                     /translation="MRPTLRRRTGITTIEFAACLALLAVFAAFLLNRLAAYEELAEKT
                     TVDLTISQIRDGIRHDLIRALLAGKRPVVNEMLERNPVSWLAQPPEAYMGEFGSLPGQ
                     ARPGAWYYVSKTHELVYVPRHSAHLNTPSSDPVRLRWRLLPAANGSGMDALEPVRIDA
                     QPAYLWF"
     gene            complement(3839..5074)
                     /locus_tag="PKX01_14270"
     CDS             complement(3839..5074)
                     /locus_tag="PKX01_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMZ77344.1"
                     /translation="MPEFAYTARDGSGAALAGRLEAGSAEAAAEQLLARGVTPVSVTP
                     ARADAAELLNQWLGRLGSTTVPTEALMLFSRQMHTLLKSGVPIMRALRGLQESAPDAR
                     LAEAVGEVRQSLESGRELASALARHPRVFSPFYVAMIRIGEVTGRLDEIFVRLFHHLE
                     FDKYMRDQVKAALRYPSFVIAVMIIALLVVNIWVIPAFAKVYEGFDAQLPILTRVLVG
                     VSAFVKNWWFLLIGGAAAMAFGFRAWVATAAGRLEWDRIKLRLPIMGKIILKATLARF
                     ARSFALAVRSGVPIVQALSVVANTVDNDHIASKIEGMRDGLERGESVLKGAIAAGIFT
                     PVVLQMIAVGEESGSLDDLLDEVGVMYQREVEFELKTLSSQIEPILIVFLGILVLVMA
                     LGIFLPIWDLGRVAMKGNG"
     gene            complement(5067..6818)
                     /locus_tag="PKX01_14275"
     CDS             complement(5067..6818)
                     /locus_tag="PKX01_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HMZ77345.1"
                     /translation="MNRPQRIRLGEVLLQQNLLTQAQLDTALEEQRRSGRKLGRIFVE
                     SGYVTEESLGTALARQLNIAFIDLRSHSPRAETVQLLSEALARRFRAIVLDDTGRALR
                     IGMTDPTDLFAYDELARVLKRELELAVVGETALLAALDRLYHRGEEINGLAKALTAEL
                     TEDASDFAELFGSGGGQEDAPVVRLLQTVFEEAVRLRASDIHVEPQERTLRIRFRIDG
                     VLYVQTEADVKIAPSVALRLKLMSGLDISEKRLPQDGRFNMTIRGAPVDVRISTLPSQ
                     YGESVVMRLLNQAAGLVSLDRIGLPDDMLVRLRRAAARPSGMILVTGPTGSGKTTTLY
                     SVLAELNSTERKIITVEDPVEYRLPGINQVQVQDKIDLSFERVLRAALRQDPDVILVG
                     EMRDKATAEIGMRASMTGHLVLSTLHTNDALSTPIRLLDMGVPRFMVAMSVQLVIAQR
                     LIRKLCTNCAEPLPLQPHERAWLRLELGSEVDAHSFRHGRGCHLCNNQGYSGRMGIYE
                     FLEMTPALVDAASHEDNRAFLEAGRAQIRGRTLRAGAMRLAAQGITSVAEAMRVANAQ
                     VEDEPDVPELEPVTRDA"
     gene            complement(6921..9728)
                     /locus_tag="PKX01_14280"
     CDS             complement(6921..9728)
                     /locus_tag="PKX01_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77346.1"
                     /translation="MPTSRHRTVALSALWRLGARCLLLLALGFTAQSVRAQTCSLPAN
                     WPSGLPGSCQCDSFNRASLNPSTIFSSNWIATTSDTTGLLPRIVNSGYLRLTDNTGQN
                     AKSATVPAAYPAAGNYISVEFRHYAYNGSGADGMTMVLSDYAVPAQPGAPGGSLGYAQ
                     NASQVGFAGGWIGIAFDEFGNYQNPTENRVGGPGFIAESVGVRGSGSGSSGYAWLTGT
                     NSLTGSNTIDNRTSTSPSRGYLYQVIVDARNYTSSNRTALFAINRDTTATGNSYASFV
                     AQFDVFARASSLGVSQSNVPTNWQISFTGSTGGSTNIHEIGALKICAQSADPPTGGAL
                     GGFNAIDSTYVRGNVNALQGHLFTKLAGTAFQLDIAALNSSSSGISSNYVLTGTKTVA
                     ISLIDDSAGTSCNASASACTACSKPVIATQNLVFNSSDQGYKTSGNFTVNGAYSRVIA
                     KMTDGTATGCSTDAFSIRPTSVSAVSATASNAALTGTPVFKAGTDAFTMTATVPVTGY
                     TGTLKLNTTYLQALPTGATAGALNPLSFTAATSGAGRSTATTSFTYSEAGAFKLLGTG
                     SNHALYDDSWTTVDQGSAGDCVAGSYSNTVDSSGKFGCLFGITADTATMGRFTASKFA
                     VTSSALTAACTASGKTAFTYMGQPFASLAVTVQAQNGAGIVLANYDSALLSSLAGVSW
                     VAEDSNSGTNLGSRMSVPAPSKTWNAGQYVVSTSAASFARAASPDGSFENLQLGVAVS
                     DPDGALLSNADFNPAGTGACSGTGCTAKAVGGTTALRFGRLALTNAYGWEKLRLPVAL
                     QAQYWNGSDFILNGDDSCTQFAATDFVLSGFTGLAACDTAFTANNSARLTSGAGQLVF
                     AAPNKTGSLTVSPTLGSVASGTTCTGSLPTSAAAATAASKTWLQGRWKGSATYTANPA
                     AKVAFGLHRGSDKIIQIREFY"
BASE COUNT         1412 a         2723 c         4027 g         1591 t
ORIGIN      
        1 ggcggcggcg atcacgaggg cgggcggacg gccgcgggcg tgggccgggg gcggcagcgg
       61 cagcaggtcg aagacgctgt cctccacacg ctgctgcagg gcgtccttga gctgccagcg
      121 cagggcgttg cgctgttcgg cctcgggtac gtcgggggtg tccacctgga ggatctggta
      181 gtcgaggccg tgcagcagcc aggtgtggcg acccttgcgc aggccgctgg cgcgggtggc
      241 ggaggcgatg cgcgcggcga gatcgccttc gacgccggcc tggcgcactg cctggtggag
      301 ctgcgggcgc ccgctgccgt cacggctgac atggacgaga tccagcgcgt cgccctggtg
      361 ggcggcggcg atccagccag tgtgtcgcgc ggagaaaaat cgtttcagca ttctggggag
      421 gcccggagga cgcatccaag ccggttcccg gcacggggcg gcaaggctta ccacgcagct
      481 taacggctgc cgccgcaagg actttaggcg cctgagcttg agaaaagcac cgtaaatccg
      541 gcccttatcg tgtgtggggc gctcgggtca gtagggaaag cttgcacgca gctggcgctc
      601 gacgtagtag cgggtgccgg cgctgccggg gcaggcgcca gcgaccggct gattgcaggc
      661 ggtggcggtg acggcgcaca ggcgcagggc ggtgccggcc tcggtgtagc tggtggtgct
      721 gacggtgacg ctgacggtga agtcggccag attgcccccc ggcgagaagc cggtggcggc
      781 gctgcagctt ccgttgcgct gggcctggta gagagcccac tccacaccgg cgcgggcggc
      841 ctggaaggcg cgggaggaga ggatgtcctg ggccgagccg gcctgctgca ggctggcgat
      901 gcgcatcagg aaggcgccca gggcggccat gatgaccagc aggaagatgg cggcgggcag
      961 ggccatgccg cgggcgtgca ggcggggttt catggggcgt tgctcacggc gacctgacgg
     1021 aagaggcgta cggactcgcc gccggaggtg agggtgaggg aggcggagac catgccggtg
     1081 cggctggcga tggcgtcata cgtgaagttg caggcgctga ccttgtcggc caccagggtg
     1141 acggtgccgc tggcgggcgg cgtgggctgg ctggccgcga tggtatagcc gctgatgcgg
     1201 gtgagggtgc cggcggcggg gttgcaggcg taggtgaccg gtgtatccac cacgaagaag
     1261 cggtgggcgg gcgagtcgaa gggaaagagg aaggatgcga agctgagggt gctgccggag
     1321 agactggcga tggtgctgcg gttgtcgccc gcataggcgt ctgcgccgct gcctgcgccc
     1381 aggttcatga ccaccaggaa ggcgccgccg ggcacgctgg ggatgctgcc gaggatgtcg
     1441 aagctggtgt cggcggcggt gaagtcgagg gggttgccga cgccggggct ggtggcggcg
     1501 atgcggtagc gcccggcgcc ggtggtctgg aggaattcca ggtagagcac gccgccgctg
     1561 gcggcgctgc ggatgctgtt gggcagcgcg gtgcggatct cccggtcgaa gcggcgcagg
     1621 gcggtgtcgg cggtgtcggt gaggtcggcg cggcgggcgg tgtcggtgta ggcgtcgatg
     1681 ggcgtgcgca ggaacacggc gatcatgcca ccgacgatgg cgagcacggt catcgtgacg
     1741 atggcctcga tgagggtgaa gccggcctcg cgcggggacc gctgctcagg ggggcgcgtt
     1801 gggtgcatag cgggtccgcc aggcgtcgag ggcgtagctg ttgccgtcgg gtgcggtgac
     1861 cgtcacggtg acgcgcaggg cctcgccggc ggcgatgccg ttgagggccg ccgcgctgac
     1921 ggcgacattg gcgctgtagc cggacaggcc ggagatggcg ttgcccgact ggtcggtgat
     1981 gccggccagg gtgaagccgg cgtagtcgtt gacgttgtcg taggggcggc tgttgccggc
     2041 ctccggcccg gagttctcgg gcaggctggc gcagccggcg gcggaggtgg cggtgtccag
     2101 gttggcatcg gccgggtcgc accaggtgaa ggcggcgcgg ctgacttcct ccagcagggc
     2161 ttcggcgatg gcgagggcct gcttgcgctg catcgggtcg gcggagccgc gcacggcggc
     2221 ggtgtaggtg gacatgaggc cggcgaggcc gaccgagacg atggcgatga acacgatcaa
     2281 ctcgatgagc gtgacgccgc gcaggggggc gggacctgcg gcgctagtgt acatagccgc
     2341 tgtccgcctc gatggtgagg gtccgggtga tgtcgccgac gatggtgagg gtgacggcgg
     2401 cgttgggccg gccgagtccg tcgaaggtga agctcgtcgg gttcgcgctg aggctgacgc
     2461 cgctcggcgg ggtcaggctg agggccgcgt tgccggcggg gtcgggcagc ggcaggtcgc
     2521 agccggcggc gccgttggtg ctgctctggg tgaaggcgag ggtgccgccg ctgtaggtgc
     2581 cgcagacggt gcggcgctgg gcgatggcga cgcggcgggc gtgctgcagg gtggcgcggg
     2641 tggtgtcggt gaagccgcgg ctgtcaaagg cgctgcggtc ggccatgcgg gggaggacgg
     2701 cgatggccag gatcccggtc aggagcagga cggcgatcag ttcgatgagg gtgaagccgg
     2761 ccgtggcgcc agcgaagggg gacttacgca tgcatgccac ggcctccggg gtcttactgg
     2821 gtgcaggtca tggtgaccgc aaaggtcttg ccagcggtgc ccttggcgtg cgcgaccgtg
     2881 cagctggtgt cggtgacgcc cgcgccggag caggtcagcg ggccaccggc gatggtgatg
     2941 ttggtcggaa cgccattggt cagcagactg ccggccaggg tcgagcacac gccgccgctc
     3001 ttgacctgca ggacgcccgt gccgctggct ccgcgggcga ccgaggtggc gtagttgagg
     3061 gaggaggcgg aggtgattgc accggccacg ccggcggcgg cggcgtcgcc cgcgtcggtg
     3121 gacaggtcca cgaacttggg cacggcgaca gctgccagga tgccgaggat gacgatgacc
     3181 acgaccagtt cgatcagggt aaagccgctt tgctgtttca tgggttcctc tcttggatgg
     3241 aatgaggttc gggacgtttg gcggtatgcc tgcggtaacg gcaggggttc gacgaggttt
     3301 agggccggaa ttgcaaaatt gatcgttgtg gttcagaacc acaggtaggc gggctgggcg
     3361 tcgatgcgca cgggctccag cgcgtccatg ccactgccat tggctgcggg cagcagacgc
     3421 cagcgcaggc gcaccgggtc gctgctgggg gtgttgaggt gggcgctgtg gcgcgggaca
     3481 tacacgagct cgtgggtctt gctgacgtag taccaggcgc caggccgggc ctggccgggg
     3541 agtgagccga attcgcccat gtaggcttcg ggcggctggg cgagccagga gacggggttg
     3601 cgttccagca tctcattcac cacggggcgc ttgccggcca gcagcgcgcg gatcaggtcg
     3661 tggcggatgc cgtcgcggat ctggctgatg gtgaggtcca cggtggtctt ttcggcgagc
     3721 tcttcgtagg cggcgaggcg gttgagcagg aaggcggcga agacggccag cagggccagg
     3781 caggcggcga actcgatggt ggtgatgccg gtccggcggc ggagcgtggg gcgcatggtc
     3841 aaccgttccc cttcatggcg acgcggccca ggtcccagat gggcaggaag atgcccaggg
     3901 ccatcaccaa tacgaggatg cccaggaaga cgatgaggat gggctcgatc tggctggaga
     3961 gggtcttcag ctcgaattcg acctcgcgct ggtacatgac gccgacttcg tccaggaggt
     4021 cgtcgaggga gccggattcc tcaccgacgg cgatcatctg gagcaccacg ggggtgaaga
     4081 tgccggcggc gatggcgccc ttgaggacgc tctcgccccg ttccaggccg tcgcgcatgc
     4141 cttcgatctt gctcgcgatg tggtcgttgt ccacggtgtt ggcgaccacg ctgagggcct
     4201 gcacgatggg cacgccactg cgcacggcga gggcgaagct gcgggcgaag cgggccaggg
     4261 tggccttgag gatgatcttg cccatgatgg gcaggcgcag cttgatgcgg tcccattcaa
     4321 ggcggccggc ggcggtggcg acccaggcgc ggaagccgaa ggccatggct gcggcgccgc
     4381 cgatgagcag gaaccaccag ttcttcacga aggcggacac gcccaccagg acccgcgtca
     4441 ggatgggcag ctgggcgtcg aagccctcgt aaaccttggc aaaggcgggg atcacccaga
     4501 tgttcaccac cagcagggcg atgatcatca cggcgatgac aaagctcggg tagcgcaggg
     4561 ccgccttcac ctggtcgcgc atgtacttgt cgaattccag gtgatggaag aggcggacga
     4621 agatctcgtc gaggcggccg gtgacttcgc cgatgcggat catggccaca tagaagggcg
     4681 agaatacgcg gggatggcgg gccagggcgg aggccagctc gcggccggat tcgagggact
     4741 ggcggacttc accgacggct tcggcgaggc gcgcgtccgg cgccgattcc tgcaggccgc
     4801 gcagggcgcg catgatgggg acgccggatt tgagcagggt gtgcatctgg cgactgaaga
     4861 gcatcagcgc ctcggtgggt accgtggtgc tgcccaggcg accgagccac tgattgagca
     4921 gctcggcggc gtcggcccgg gcgggggtga ccgacacggg ggtgacgccg cgggcgagga
     4981 gctgctcggc ggcggcctcg gcggagccgg cttcgaggcg gccggccagg gcggcgccgc
     5041 tgccgtcgcg ggcggtgtag gcgaactcag gcatcgcggg tcacgggttc cagttcgggc
     5101 acgtccggtt cgtcttccac ctgggcattg gccacgcgca tggcttcggc caccgaggtg
     5161 atgccctggg cggcgaggcg catggcgccg gcgcgcaggg tgcgtccgcg gatctgggcg
     5221 cggccggctt cgaggaaggc gcggttgtcc tcgtggctgg cggcgtccac aagggccggc
     5281 gtcatttcga gaaattcgta gatgcccatg cgcccgctgt agccctggtt gttgcacagg
     5341 tggcagccgc ggccgtggcg gaagctgtgg gcgtccacct cgctgcccag ctcgaggcgc
     5401 agccaggcgc gctcatgggg ctgcaggggc agcggttcgg cgcaattggt gcagagcttg
     5461 cggatcaggc gctgggcgat gacgagctgc acggacatgg cgaccatgaa gcgcgggacg
     5521 cccatgtcga gcaggcggat gggcgtggac agggcgtcgt tggtgtgcag cgtggacagc
     5581 accaggtggc cggtcatgga ggcgcgcatg ccgatctcgg cggtggcctt gtcgcgcatt
     5641 tcgccgacga ggatgacgtc cgggtcctgg cgcagggcgg cccgcaggac gcgctcgaag
     5701 gacaggtcga tcttgtcctg cacctgcacc tggttgatac cgggcaggcg gtattccacc
     5761 gggtcttcca cggtgatgat cttgcgctcg gtgctgttga gttcggcgag cacggaatag
     5821 agggtggtgg tcttgccgct gccggtgggg ccggtgacga ggatcatgcc gctggggcgg
     5881 gcggcggcgc ggcgcaggcg tacgagcatg tcgtcgggca ggccgatgcg gtcgaggctg
     5941 acgaggccgg cggcctggtt gagcagacgc atcaccacgg attcgccgta ctggctgggc
     6001 agtgtcgaga tgcgcacgtc caccggtgcg ccgcggatgg tcatgttgaa gcggccgtcc
     6061 tggggcaggc gcttttcgga gatgtccaga ccggacatga gcttgaggcg cagggcgacg
     6121 gacggggcga tcttcacgtc ggcctcggtc tgcacgtaga gcacgccgtc gatgcggaag
     6181 cggatgcgca gggtgcgctc ctggggctcc acgtggatgt cggaggcgcg caggcgcacg
     6241 gcttcctcga agacggtctg gagcaggcgc accaccggcg cgtcttcctg gccgccgcca
     6301 gagccgaaga gttcggcgaa gtcgctggcg tcttcggtga gttcggcggt gagggccttg
     6361 gccaggccgt tgatctcttc gccccggtgg tagaggcggt ccagcgcggc gaggagggcg
     6421 gtttcgccga cgacggcgag ctccagttcg cgcttgagga cgcgggccag ttcgtcgtag
     6481 gcgaagaggt ccgtgggatc ggtcatgccg atgcgcaggg cgcgcccggt gtcgtccagc
     6541 acgatggcgc ggaagcgtcg ggcgagggct tcggacagca gctggacggt ctcggcccgc
     6601 gggctgtggc tgcgcaggtc gatgaaggcg atgttgagct ggcgggcgag ggcggtgccg
     6661 agggattcct cggtgacgta gccgctctcc acgaagatgc ggcccagctt gcggccgctg
     6721 cggcgctgct cttccagggc ggtgtcgagt tgcgcctggg tgaggaggtt ctgttgcagg
     6781 aggacttcgc cgagacggat gcgctgcggt ctgttcatgg cctgggctgg gcgtgcgttg
     6841 ccggaagggg cggtcgattt cccggaatta cggcggggga ccgttcgact tgagggcgag
     6901 ggcgggcttc cgatgctgca tcaatagaat tcgcggatct ggatgatctt gtcggagccc
     6961 cggtgcaggc cgaaggcgac cttggcggcg ggattggcgg tgtaggtggc gctgcccttc
     7021 cagcggccct ggagccaggt cttggacgcc gccgtggcag cggccgcgga ggtgggcagg
     7081 ctgccggtgc aggtggtgcc gctggcgacg ctgcccaggg tgggggagac ggtgaggctg
     7141 ccagtcttgt tgggcgcggc gaagacgagc tgaccggcgc cgctggtgag tctggcgctg
     7201 ttgttggcgg tgaaggcggt gtcgcaggcg gccaggccgg tgaaaccgga caggacgaag
     7261 tcggtggccg cgaactgggt gcaggagtcg tcgccgttga ggatgaagtc gctgccgttc
     7321 cagtactggg cctgcagcgc caccggcagg cgcagtttct cccagccgta ggcgttggtg
     7381 agggcgaggc ggccgaagcg cagggcggtg gtgccgccca cggccttggc ggtgcagccg
     7441 gtgccggagc aggcgccggt gccggcggga ttgaaatcgg cgttgctgag cagggcgccg
     7501 tccgggtcgc tgacggcgac gcccagctgc aggttttcga agctgccgtc aggcgatgcc
     7561 gcgcgggcga agctggcggc gctcgtgctg accacgtact ggccggcgtt ccaggttttg
     7621 ctgggcgccg ggacgctcat gcggctgccc agattggtgc cgctgtttga atcctccgcc
     7681 acccacgaga cgccggcgag gctggaaagc agggccgaat cgtagttggc gagcacgatg
     7741 ccggcgccgt tctgggcctg cacggtgacg gccaggctgg cgaagggctg acccatgtag
     7801 gtgaaggcgg tcttgccgct ggcggtgcac gcggcggtga gggcgctgct cgtcacggcg
     7861 aacttgctgg cggtgaagcg gcccatcgtc gcggtgtcgg cggtgatgcc gaacaggcag
     7921 ccgaatttgc cgctgctgtc caccgtgttg ctgtagctgc cggccacgca gtcgccggcg
     7981 gagccctggt ccacggtggt ccagctgtcg tcgtagaggg cgtggttgct gccggtgccc
     8041 agcagcttga aggcgccggc ttcgctgtag gtgaagctgg tggtggcggt ggatctgccc
     8101 gcgccgctgg tggcggcggt gaagctgagg ggattgaggg caccggccgt ggcgccggtg
     8161 ggcagggcct ggaggtaggt ggtgttgagc ttgagggtac cggtgtagcc ggtgacgggg
     8221 accgtggcgg tcatcgtgaa ggcgtcggtg ccggccttga agacaggggt gccggtgagg
     8281 gcggcattgc tggcggtggc gctgaccgcc gaaacggagg tggggcggat gctgaaggcg
     8341 tcggtggagc agccggtggc ggtgccgtcg gtcatcttgg cgatcacccg tgaataggcg
     8401 ccgttgacgg tgaagttgcc gctggtcttg tagccctggt cggatgaatt gaagaccagg
     8461 ttctgggtgg cgatgaccgg cttgctgcag gccgtgcagg cgctggccga ggcgttgcag
     8521 gaggtgccgg cgctgtcgtc gatgaggctg atggcgacgg tcttggtgcc ggtcaggacg
     8581 tagttggacg atatgcccga gctgctgctg ttgagggctg caatatccag ctggaacgcc
     8641 gtgccggcca gcttggtgaa caggtggccc tgcagcgcat tcacgttgcc gcggacgtag
     8701 gtggagtcga tggcattgaa gccgcccagg gcgccaccgg tgggcgggtc ggcactctgg
     8761 gcgcagatct tgagggcgcc gatctcatgg atgttggtag agccgccggt ggagccggtg
     8821 aaggagatct gccagttggt gggcacattg gactggctga cgcccaggct ggatgcgcgg
     8881 gcgaagacgt cgaactgggc cacgaagctg gcgtaggagt tgccggtggc ggtggtgtcc
     8941 cgattgatcg cgaagagggc ggtgcggttg ctggaggtgt agttgcgggc atcgacgatg
     9001 acctgataca ggtaaccgcg ggacggggag gtggacgtgc ggttgtcgat ggtgttgctg
     9061 ccggtgaggc tgttggtgcc ggtgagccag gcgtagccgc tggagccgga accggagccc
     9121 cgcacgccga ccgattcggc gatgaagccg ggaccgccga cgcggttttc ggtggggttc
     9181 tggtagttgc cgaattcgtc gaaggcgatg ccgatccagc cgccggcgaa gcccacctgg
     9241 gacgcattct gcgcgtagcc cagagacccg cccggggcgc ccggctgggc ggggacggcg
     9301 tagtccgaca gcaccatcgt catgccgtcc gcgccggagc cgttgtaggc gtagtggcgg
     9361 aattccaccg aaatgtagtt gcctgcggcc ggataggcgg ccggcacggt ggcggatttg
     9421 gcgttctggc cggtgttgtc ggtgaggcgc aggtagccgg aattgacgat ccgcggcagc
     9481 agaccggtgg tgtcgctggt ggtggcgatc cagttgctgc tgaagatggt ggacggattg
     9541 aggctggcgc ggttgaagct gtcgcactgg caactgcccg gcaggccgct cggccagttg
     9601 gccggcaggg agcaggtctg ggcccgcacg gactgggcag tgaagcccaa tgcgagcagg
     9661 agcaggcacc gcgcgccaag gcgccagagg gcggacaggg caaccgtacg gtggcgggaa
     9721 gttggcatgg cgtcgggcgg atccgtaacg atg
//
LOCUS       DAOQPA010000123         9590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3806_length_9590_cov_18.706765, whole genome shotgun sequence.
ACCESSION   DAOQPA010000123 DAOQPA010000000
VERSION     DAOQPA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3806_length_9590_cov_18.706765"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKX01_14285"
     CDS             <1..1358
                     /locus_tag="PKX01_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ77347.1"
                     /translation="GMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWWRIMKQFNP
                     KNAKSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALP
                     TEFSARVARNTQIIIQEETHICNVVDPWAGSYMMEKLTQDMADTAWALIEEIEAMGGM
                     TKAVESGWAKMKVEECAAEKQARIDSGKDVIVGVNKYKLAKEDPIEILDIDNHAVREA
                     QIERLARIRATRDQAAVDAALAALTACAKSGEGNLLDLTVKAIRLRATVGEVSDALEV
                     EFGRYRANPQAVSGIYNAVVEGNDDWKALKADIEAFIAEEGRRPRIMIAKLGQDGHDR
                     GAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHAVGCSSLAAGHKTLVPAIIK
                     ALHEQGADDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPTAAREVLKQIRAARVA
                     A"
     gene            1422..2420
                     /gene="meaB"
                     /locus_tag="PKX01_14290"
     CDS             1422..2420
                     /gene="meaB"
                     /locus_tag="PKX01_14290"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMZ77348.1"
                     /translation="MNICADLPQRDAADQALVDGVLRHQLRPLAKTITLIESQRADHK
                     RRAQAVLEGLLPASGRSMRVGISGVPGVGKSTFIEALGLYLIEQGHKVAVLAVDPSSS
                     VTGGSILGDKTRMELLSQNPAAFIRPSPSAGSLGGVAEKTREAMLVCEAAGFDVIIIE
                     TVGVGQSETAVAGMTDIFCLLQLPNAGDDLQAIKKGIMEIADLIVINKADINPQAATV
                     ARAQIKSALGMLRHTSPHWTPPVLMLSALQKQGVAEFWKTVNDYRTTMQASGEFDSRR
                     RHQAQAWMWDLIDQGLRARFAGHPAVQNDLPALQAAVEAGRATPSAAAARLLGHLG"
     gene            2468..4003
                     /locus_tag="PKX01_14295"
     CDS             2468..4003
                     /locus_tag="PKX01_14295"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMZ77349.1"
                     /translation="MHDIIHQLEKKREAARLGGGQKRIDSQHKKGKLTARERIELLLD
                     PGSFEEWDMFKEHRCVDFGMDQADKSPGDGVIVGYGTVNGRLVFVFSQDFTVFGGSLS
                     ETHAEKICKVMDHAMKVGAPVIGINDSGGARIQEGVASLGGYADVFQRNVMASGVVPQ
                     ISMIMGPCAGGAVYSPSMTDFIFMVKDSSYMFVTGPEVVKTVTHEDVTAEELGGAVTH
                     TSKSGVADLAFENDVEALSMLRRFMNFLPANNREKPPVQSTNDPVDRMDYSLDTLVPD
                     NANKPYDMKELLVKVVDDSDFFELQADYAKNIIIGFGRIDGHPVGIVANQPLVLAGCL
                     DIKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTAQEYGGIIKHGAKLLYAYAECTV
                     PKVTVITRKAYGGAYDVMSSKHLRGDVNLAWPSAEIAVMGPKGAVEIIFREEKNDPAK
                     LAQREAEYKAKFANPFVAGARGFIDDVIMPHATRKRIARSLAMLRDKKLENPWRKHGN
                     IPL"
     gene            4023..6026
                     /gene="accC"
                     /locus_tag="PKX01_14300"
     CDS             4023..6026
                     /gene="accC"
                     /locus_tag="PKX01_14300"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ77350.1"
                     /translation="MFTKILIANRGEIACRVIKTARKMGIQTVAVYSEADRDALHVEL
                     ADEAVCIGPAASKESYLVMDKIIAACKQTGAQAVHPGYGFLSENAEFSRRLEEEGIKF
                     IGPKHYSVAKMGDKIESKKLAIAAQVNTIPGYNDAIAGPAEAVEIAKKIGYPVMIKAS
                     AGGGGKGLRVAYNDAEAFEGFSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDAH
                     GNYLYLNERDCSIQRRHQKVIEEAPSPFVDAEMRKAMGEQAVALARAVNYESAGTVEF
                     VVSGATKEFYFLEMNTRLQVEHPVTELITGLDLVEQMIRVAYGEKLPISQADVGINGW
                     AMECRINAEDPFRGFLPSTGRLVKFQPPADVDGQVRVDTGVYDGGEISMYYDSMIAKL
                     IVHGATRDQAIARMRDALNGFVIRGISSNIPFQAALMQHPRFQSGIFDTGFIAKEYPK
                     GFDASMVPHDDPALLVSVAAYVYRAYTDRSASVSGQLQGHERIVGDHWMVIRLSKDGN
                     EQHPVVARPIDGGYHVEYKGESYELKSDWKLGESLFNGTCNGEPFTLQVERNRTRYSL
                     YHWGTRADFMVMSGRAAELLALMPEKLPPDLSKFLLSPMPGLLREVAVKVGQDVKAGE
                     KLAVIEAMKMENILKAEQDCKVKSIVAAAGASLAVDEVIIEFE"
     gene            6468..7214
                     /locus_tag="PKX01_14305"
     CDS             6468..7214
                     /locus_tag="PKX01_14305"
                     /inference="COORDINATES: protein motif:HMM:NF006562.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HMZ77351.1"
                     /translation="MNKALVIVKVGDAVAELIPDHGDYDAWIRDGFGPTRLPVRVVDP
                     RVGEALPTAPGVAGVVVTGSDAMVTDRAGWSEATARWLADIVAHRVPVLGICYGHQLL
                     AHALGGMVIYHPAGKEIGTVPVEQTDIARDDPLFADLPERFPAHVVHSQTVAILPPGA
                     VILASNDFEPHHAFRVGPSAWGVQFHPEFSVAATRACIVESERSLRLSGTDPAALADA
                     VTETPEATSLLARFARFVDDAQTPERPSDA"
     assembly_gap    7605..7704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8152..9546
                     /locus_tag="PKX01_14310"
     CDS             8152..9546
                     /locus_tag="PKX01_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77352.1"
                     /translation="MSARQTSLFPRARFSLIGLAVFAASAAAHAGVFDTVTEGIGNLK
                     GKLTGGAAEAGATGKPASPEAVANEPAPDPSTFKAVVPTVAVENIDALPKSKKIILSN
                     FVVEFQQQYEKRKTGFSIMGLGNAGSSTAKMDATLPSQATLQAITNFAYLDTVKKLRA
                     RGYEVIEVTQLSEKSRGSYEKLAKSNPIKSGEVFDNIDGQSVLVSPDGMTSTIPNAGC
                     THFGSRKAGANLSNNLRQTSSGAQTKYENEVANGEGKIPLLKVWIAVEFGEVEANGGN
                     AFISARQRDFLGSTTTTISNSANAQATQGMFLEPGVTHFSFELPVDGEKYNHGCGISL
                     SSSTVTPPADGDVFIRLKEKYRDDGDSAPLKLSSQAGNVAVTDTYIGGGIGIRSVKDS
                     DKGEQARTFDNGKGTSVVQHAGRTSGTVDVAGMGTSLHTSREWTAEIRSDVYAASAAT
                     MIYKVTDAFIARLP"
BASE COUNT         1819 a         3375 c         2803 g         1493 t
ORIGIN      
        1 ccggcatgga cgtggacacc ttcgccggcc gcctgtcctt cttctgggcg gtggggatga
       61 acttctacct cgagatcgcc aagatgcgcg ccgcccgcct gctgtggtgg cgcatcatga
      121 agcagttcaa tcccaagaac gccaagtcca tgatgctgcg gacccacagc cagacctccg
      181 gctggtccct caccgagcag gacccgtaca acaacgtggt gcgcaccacc atcgaagcca
      241 tggcggccgt gttcggcggc acccagtccc tgcacaccaa cgccctcgac gaagccatcg
      301 cgctgcccac cgagttctcc gcccgcgtcg cccgcaacac ccagatcatc atccaggaag
      361 aaacccacat ctgtaacgtc gtcgacccct gggccggctc ctacatgatg gaaaagctca
      421 cccaggacat ggccgacacc gcctgggccc tgatcgaaga gatcgaggcc atgggcggca
      481 tgaccaaggc cgtcgaatcc ggctgggcca agatgaaggt cgaagagtgc gccgctgaga
      541 agcaggcccg catcgactcc ggcaaggacg tcatcgtcgg cgtcaacaag tacaagctgg
      601 ccaaggaaga tcccatcgag atcctcgaca tcgacaacca cgccgtgcgt gaagcccaga
      661 tcgagcgtct cgcccgcatc cgcgccaccc gcgaccaggc tgccgtggat gccgccctgg
      721 ccgccctcac cgcctgcgcc aagagcggcg aaggcaacct gctcgacctc accgtcaagg
      781 cgatccgcct gcgcgccacc gtcggcgaag tctccgacgc gctggaagtc gagttcggcc
      841 gctaccgcgc caacccgcag gccgtgtccg gcatctacaa cgccgtggtc gaaggcaacg
      901 acgactggaa ggccctcaag gccgacatcg aagccttcat cgccgaagaa ggccgccgtc
      961 cgcgcatcat gatcgccaag ctcggccagg acggtcacga ccggggcgcc aaggtggtgg
     1021 cctcggcctt cgccgacctg ggcttcgaca tcgacatcgg gcccctcttc cagaccccgg
     1081 aagaggccgc ccgccacgcc gtcgagaacg acgtgcacgc cgtcggctgc tccagcctcg
     1141 ccgccggcca caagaccctc gtgccggcca ttatcaaggc cctccacgag cagggcgccg
     1201 atgacatcat caccttcgtc ggcggcgtga tccccgccca ggactacgac gccctgtatg
     1261 ccgcgggcgc caagggcatt ttcgggccgg gcacgccgat ccccacggct gcccgcgaag
     1321 tgctcaagca aattcgcgcc gcccgcgtcg ccgcctgaag cgtagaatca cggattcacc
     1381 cgccggtgca gtatgccggc gggtgaatcg ttgatcattc catgaacatc tgcgccgacc
     1441 ttccgcaacg ggacgccgcc gaccaggccc tggtcgacgg tgtgctgcgt catcagctgc
     1501 gcccgctggc caagaccatc accctcatcg agtcccagcg cgccgaccac aagcggcgcg
     1561 cccaggcggt gctcgaaggt ctcctgccgg cgagcggacg ctccatgcgg gtgggcatct
     1621 ccggtgtgcc cggtgtgggc aagtccacct tcatcgaagc gctgggcctg tacctcatcg
     1681 agcagggtca caaggtggcg gtgctggcgg tggatccgtc ctcgtcggtg accggcggtt
     1741 ccatcctggg cgacaagacc cgcatggagc tgctgtccca gaatcccgcc gccttcatcc
     1801 gccccagccc gtccgcgggc agcctgggcg gcgttgccga aaagacccgt gaggcgatgc
     1861 tggtgtgcga ggcggccggt ttcgacgtca tcatcatcga gaccgtcggc gtcggccagt
     1921 ccgaaaccgc ggtggcgggc atgaccgaca tcttctgcct gctccagctc cccaacgccg
     1981 gtgacgacct gcaggcgatc aagaaaggca tcatggagat cgccgacctg atcgtcatca
     2041 acaaggccga catcaacccc caggcggcca ccgtcgcgcg ggcgcagatc aagagcgccc
     2101 tgggcatgct gcgtcacacg tcaccccact ggacgccgcc ggtgctgatg ctctcggcgt
     2161 tgcagaaaca gggcgtcgcc gagttctgga aaacggtcaa cgactatcgc accacgatgc
     2221 aggccagcgg cgagttcgac agccgccggc gacatcaggc ccaggcctgg atgtgggatc
     2281 tcatcgacca aggcctgcgt gcccgctttg cggggcaccc cgccgtacaa aacgatctgc
     2341 cggccctgca ggccgccgtc gaagccgggc gggccacccc gtccgcggcg gcggcgcggc
     2401 tgctgggaca tctgggctga catcattcaa aagagaaatc gacaggatca ttgactggag
     2461 gctgtaaatg cacgacatca tccaccagct ggaaaagaag cgcgaagcgg cccgcctcgg
     2521 gggcggccag aagcggatcg acagccagca taaaaaaggc aagctgacgg cccgcgaacg
     2581 gatcgaactg ctcctcgacc cgggttcctt cgaagaatgg gacatgttca aggagcaccg
     2641 ctgcgtcgac ttcggcatgg atcaggccga caagtccccc ggtgacggcg tcatcgtcgg
     2701 ttacggcacc gtcaatggcc gcctggtgtt cgtgttcagc caggacttca ccgtgttcgg
     2761 cggctccctg tccgagaccc acgccgagaa gatctgcaag gtcatggacc acgccatgaa
     2821 ggtcggcgcc ccggtcatcg gcatcaatga ttccggcggc gcccgcatcc aggaaggcgt
     2881 cgcctccctc ggcggctacg ccgacgtgtt ccagcgcaac gtgatggcct ccggcgtcgt
     2941 gccccagatc tccatgatca tgggcccctg tgccggcggt gccgtgtatt ccccgtccat
     3001 gaccgacttc atcttcatgg tcaaggactc ctcctacatg ttcgtgaccg gtcccgaagt
     3061 cgtgaagacc gtgacccacg aagacgtcac cgccgaggag ctgggcggcg ccgtcaccca
     3121 caccagcaag tccggcgtgg ccgacctggc cttcgagaac gacgtggaag ccctctccat
     3181 gctgcgtcgt ttcatgaact tcctgcccgc caacaaccgc gagaagccgc ccgtgcaatc
     3241 gaccaacgac ccggtcgacc gcatggacta ctccctcgac accctggtgc cggacaacgc
     3301 caacaagccc tacgacatga aggaactgct cgtcaaagtg gtggacgaca gcgacttctt
     3361 cgagcttcag gccgactacg ccaagaacat catcatcggc tttggccgca tcgacggtca
     3421 cccggtgggc atcgtcgcca accagcccct ggtgctggcc ggctgcctgg acatcaagtc
     3481 ctccatcaag gccgcccgct tcgtgcgctt ctgcgacgcc ttcaacatcc ccgtcgtcac
     3541 ctttgtcgac gtgcccggct tcatgcccgg caccgcccag gaatacggcg gcatcatcaa
     3601 gcacggcgcc aagctgctct acgcctacgc cgaatgcacc gtgcccaagg tcaccgtcat
     3661 cacccgcaag gcctacggcg gcgcctatga cgtgatgtcc tccaagcacc tgcgcggcga
     3721 cgtcaacctc gcctggccct ccgccgaaat cgcggtgatg ggccccaagg gcgcggtgga
     3781 gatcatcttc cgcgaagaaa agaacgaccc cgccaagctc gcccagcgcg aagccgagta
     3841 caaggccaag ttcgccaacc ccttcgtggc cggcgcccgc ggcttcatcg acgacgtgat
     3901 catgccgcac gccacccgca agcgcatcgc ccgctcgctg gccatgctgc gcgacaagaa
     3961 actggaaaat ccgtggcgca agcacggcaa catcccgctc tgatcgccga ggacatcaag
     4021 acatgtttac caagatcctg attgcgaacc gcggcgagat tgcttgccgc gtcatcaaga
     4081 ccgcccgcaa gatgggcatc cagaccgtcg ccgtgtattc cgaggccgac cgggacgccc
     4141 tgcacgtgga gctggccgac gaagccgtgt gcatcggacc ggccgcctcc aaggaatcct
     4201 acctggtcat ggacaagatc atcgccgcgt gcaagcagac cggcgcccag gctgtccacc
     4261 ccggctacgg cttcctctcc gagaacgcgg aattctcccg ccgcctcgaa gaagaaggca
     4321 tcaagttcat cggccccaag cactactccg tggccaagat gggcgacaag atcgagtcca
     4381 agaagctcgc catcgccgcc caggtcaaca ccatccctgg ctacaacgac gccattgccg
     4441 gcccggccga agccgtcgag atcgccaaga agatcggcta cccggtgatg atcaaggcct
     4501 ccgccggcgg cggcggcaag ggtctgcgcg tggcctacaa cgacgccgaa gccttcgaag
     4561 gcttctcctc ctgcgtcaat gaagcccgca actccttcgg cgacgaccgc gtcttcatcg
     4621 aaaaatacgt cctcgaaccg cgccacatcg aaatccaggt gctgggcgac gcccacggca
     4681 attacctgta cctcaacgag cgcgactgct ccatccagcg ccgccaccag aaggtcatcg
     4741 aagaagcgcc gagccccttc gtggacgccg aaatgcgcaa ggccatgggc gaacaggccg
     4801 tggccctggc ccgcgcggtg aactacgaat ccgcgggcac cgtcgaattc gtcgtctccg
     4861 gcgccaccaa ggagttctac ttcctcgaga tgaacacccg tctccaggtg gaacacccgg
     4921 tcaccgaact catcaccggc ctcgacctcg tcgagcagat gatccgcgtc gcctacggcg
     4981 aaaagctccc catcagccag gccgacgtgg gcatcaacgg ctgggccatg gagtgccgca
     5041 tcaacgccga agaccccttc cgcggcttcc tcccctccac cggtcgtctg gtcaagttcc
     5101 agccgcccgc cgatgtggac ggccaggtgc gtgtggatac cggcgtgtac gacggtggcg
     5161 agatctccat gtactacgac tcgatgatcg ccaagctcat cgtccacggc gccacccgcg
     5221 accaggccat tgcccgcatg cgcgacgccc tcaacggctt cgtgatccgc ggcatctcct
     5281 cgaacatccc gttccaggcc gcgctcatgc agcacccgcg cttccagtcc ggcatcttcg
     5341 acaccggctt catcgccaag gaatacccca agggcttcga cgcctccatg gtgccccacg
     5401 atgatcccgc cctgctggtc tccgtcgccg cctacgtgta ccgcgcctac accgaccgct
     5461 ccgcctccgt gagcggccag ctgcaaggcc acgaacgcat cgttggcgac cactggatgg
     5521 tcatccgcct cagcaaggac ggcaacgagc agcaccccgt cgtcgcccgt cccatcgacg
     5581 gcggctacca cgtggaatac aagggcgaga gctacgagct caagtccgac tggaagctcg
     5641 gcgagtccct cttcaacggc acctgcaacg gcgaaccctt caccctccag gtggagcgca
     5701 accgcacccg ctacagcctc taccactggg gcacccgcgc cgacttcatg gtcatgagcg
     5761 gtcgtgccgc cgagctgctg gccctcatgc ccgagaagct gccgcccgac ctctccaagt
     5821 tcctgctgtc ccccatgccc ggcctgctgc gcgaagtggc cgtcaaggtc ggccaggacg
     5881 tgaaggccgg cgaaaagctc gccgtcatcg aagccatgaa gatggaaaac atcctcaagg
     5941 ccgagcagga ctgcaaggtc aagtccatcg tcgctgccgc cggcgccagc ctcgcggtgg
     6001 acgaagtcat catcgagttc gagtaatcgg ctccggccgc cgcgcgctgc tccggcgcgc
     6061 aggcggccgc caggttttca ttcgatgggg agggagactc gtccttcggg gcgggttcct
     6121 gtcgagggag tggggtcgtc ctaggacggc cccttttttt tgcctgcgcg gtcccgaccg
     6181 caccgataca gtgcagcgac gaccatcgtc gccccgtccg gatcgaatct cacgccgggc
     6241 gcacctgcgc cgggcatttc ggccagccgg cacgggtttt tcgcagtgca gcatgccggg
     6301 cgcgtgcaag ggattgcgcc cggatcgcca tggccgggaa tttgcttaac attcgcatac
     6361 ccataatgag gtggacacgc acgacgcgga tggcaacgcg tctgcaccat gcgccaccct
     6421 cctggccgga gcgcccggca ggcccccgat tgcaaatgga gagcgatgtg aacaaagccc
     6481 tggtgatcgt caaggtcgga gacgccgtcg cggagctcat ccccgaccac ggcgactacg
     6541 acgcctggat ccgtgacggc ttcggcccca cccgcctgcc cgtgcgcgtc gtcgatcccc
     6601 gcgtcggcga agccctgccc accgcgcccg gcgtcgccgg cgtcgtcgtc accggctccg
     6661 acgccatggt caccgaccgc gccggctgga gcgaagccac cgcccgctgg ctcgccgaca
     6721 tcgtcgccca ccgcgtcccc gtgctcggca tctgctacgg ccaccagctc ctcgcccacg
     6781 ccctgggcgg catggtcatc taccacccgg ccggcaagga aatcggcacg gtccccgtcg
     6841 aacagaccga catcgcccgc gacgaccccc tctttgccga tctccccgag cgcttccccg
     6901 cccacgtcgt gcacagccag accgtcgcca tccttccccc tggcgccgtc atcctcgcca
     6961 gcaacgactt cgaaccccac cacgccttcc gcgtcggccc ctccgcctgg ggcgtccagt
     7021 tccaccccga attcagcgtc gccgccaccc gcgcctgcat cgtcgaaagc gaacgcagcc
     7081 tgcgcctcag cggcaccgac cccgccgccc tcgccgacgc cgtcaccgaa acccccgagg
     7141 ccaccagcct gctggcgcgc tttgcccgct tcgtggacga cgcgcagacc cccgagcggc
     7201 cctccgacgc ctgaaaaggc gccgcggaca cttcagcgca cgtcccgccc ccctccccgg
     7261 agcccctgca cgccgggcgg caggatcgtc cagatcctcg gggccatctc cgcagtgtcc
     7321 acgaccttcg gcaacgcatg ccgcacgtcg ggccgtatcc gcagaagccc ccgaacagat
     7381 tccgcacgta ccggcacgat gtgcccgagc gtccagaatc cgtttcagcc atcgctcacc
     7441 ggctgcccct catcccgcca ttcttccggg gcgcctctcg cgccctgcca gccgtctggc
     7501 gaacatcgcc cggcgtgtag cacgacgtgc cagacgtggg gacctgccgt gccagacgcc
     7561 gggcgagcgc tgccagacgt ctggcacggc tcgcttggcg tctannnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnacggct cgcttggcgt ctagctgccc tgattgacgc
     7741 ccatcgaggc tcggctggcg tctggcacga catacctgcg tgtgtggcaa agcttgccag
     7801 atgcctggcg agggtcgccc ggcgtctagc ggggctgatt gacgcctatt gaagtacgcc
     7861 agacgtctgg ggcgacgatt ccccgtaact ggcaaggctt gctagggcgc gggcgagtgt
     7921 tgctaggcgc gtagcgaaaa tggccagccg aggggaggac ggatgatcgg ccattgctgc
     7981 ggtgcatgcc cggtgcatgg gaggcgtttt gggacaaaac acagcccctc tgcctataat
     8041 gcctcgctca atgccttgtc tagacacgac aaattcgatc ctggcgggcg tcggcatatt
     8101 cgcactgatc aatcattcga tgccacgcgt ggcagaaaga gaaagtccac catgtcagcc
     8161 cgccagacaa gcttattccc ccgggcccgt ttctccctta tcggtctcgc cgtattcgct
     8221 gccagtgcag cagcgcatgc cggggtgttc gataccgtca cggaaggtat tgggaacctg
     8281 aaaggcaagc tcacgggagg cgctgcagaa gccggagcga ccggcaagcc ggcgagcccc
     8341 gaagcagtgg caaatgaacc cgcgcccgat cccagcactt tcaaggctgt tgtcccgacc
     8401 gtggctgttg aaaacatcga tgcgctgccc aagagcaaga agatcatcct gagcaatttc
     8461 gtggtggagt tccagcagca gtacgaaaag cgaaagacag gcttcagcat catgggcctg
     8521 ggcaatgcgg gttcgagtac cgccaagatg gatgcgacgc tgcccagcca ggccaccctg
     8581 caggccatta cgaattttgc ctatctcgac accgtcaaga agctcagggc gcggggctat
     8641 gaagtcatcg aagtgaccca gctttccgag aaatcccgtg gcagctacga gaagctcgcc
     8701 aagtccaatc cgatcaaatc cggcgaggtc ttcgacaata tcgacggtca aagcgtgctg
     8761 gtcagcccgg acggtatgac ctcgaccatt ccaaacgccg gctgcacgca cttcgggtcc
     8821 cgaaaggccg gcgcgaacct gtccaacaac ctgcggcaga catcctccgg cgcgcagacg
     8881 aagtatgaaa acgaagtggc caatggggag ggaaagattc ctctgctgaa ggtgtggatt
     8941 gccgttgaat tcggcgaagt ggaagccaat ggcggcaatg cgttcattag cgcacggcag
     9001 cgcgacttcc ttggctcaac cacaaccacg atcagcaact ctgccaatgc gcaggctacg
     9061 caaggcatgt tcctcgaacc cggcgtcacg catttttcct tcgaattgcc cgtcgacgga
     9121 gagaagtaca accatggctg cggcatcagc ttgagcagca gtacggtgac cccgccggcc
     9181 gacggcgacg tgttcatccg tctcaaggaa aagtatcgtg acgatggcga cagcgcgccc
     9241 ctgaaactga gcagccaggc cggcaacgtg gccgtgaccg acacgtatat cggcggtggc
     9301 atcggcattc gaagcgtcaa ggacagcgat aagggcgaac aggcccgaac gttcgacaac
     9361 ggcaagggga cttccgtcgt ccagcatgcg gggcgcacct cgggcaccgt cgatgttgcg
     9421 ggcatgggta cgtctctgca tacgagccgg gaatggaccg ccgagatccg ctcggatgtg
     9481 tatgcggcga gtgccgccac catgatctac aaggtgaccg acgcttttat cgccagactg
     9541 ccttgatggg tgcaggccgc ggcagggggt gccctgagcg cggcctttgc
//
LOCUS       DAOQPA010000124         9438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3890_length_9438_cov_19.656400, whole genome shotgun sequence.
ACCESSION   DAOQPA010000124 DAOQPA010000000
VERSION     DAOQPA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_9438_cov_19.656400"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PKX01_14315"
     CDS             <1..1511
                     /locus_tag="PKX01_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HMZ77353.1"
                     /translation="TTDAAGNSASAASSFVEYLHAPTAPTLTLSTPTVPTLSNGLIRD
                     TWNASVGPYNSGNGVAPTTMEASIDALIAAGTAPNTSTVLTNAAVASVAVGVASETHG
                     LIYLTGGTAYTFQIKGDDSILLEIGGQTVGSVTWGTGSLANGKFTPTTSGWYTLDLYH
                     DNEAGPGNFTVRVSTNGSSFVNLDTTNFQLAQSVQDLNSAGIRTSSLQGSSTTEPNDG
                     YYTVLPYNEGHEAAWIPLSTVAGSLVSPGASDVLNIHITGLAQNTQLTDGTHWATADA
                     SGTVDVTGWTMNALSALAPVDYSGTMNAQVQAIATDTLNGVSTTTNKALAIVVDPYVT
                     VAGTTGTGTDDRIVGTSGADTLKGGEGSDLIIGGAGSDTLTGGAGSDVFRWMLADKGT
                     PGLPAQDTITDWSSAAVKNGGDVLDLRDLLQGESHGASGLGNLTSYLHFEKVGTATVV
                     DISSSGGYSAGFKASATDQTIVLSNTDLTSGGTLNDAQIIQDLMTKGKLHTD"
     gene            complement(1659..3473)
                     /gene="typA"
                     /locus_tag="PKX01_14320"
     CDS             complement(1659..3473)
                     /gene="typA"
                     /locus_tag="PKX01_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMZ77354.1"
                     /translation="MSRSIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVSERVMD
                     SNDLEKERGITILAKNCAIQYGDTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVINKIDRPGSRPDWVINHTFDLFDKLGATEEQLDF
                     PVVYASALNGYAMLDADQPGTDMKPLYEAILQHVPEPDVDAEAPLQLQICSLDYSTYI
                     GQLGIGRIKRGRIRPNQEVAVMYGDESRGKAKISQILTFTGLDRAPAESAEAGDIVLV
                     SGIDQVNIGVTLCAPESIEPLPPIAVDEPTLTMNFMVNSSPLAGREGKFVTSRQIRER
                     LEKELLKNVALRVNFTSDSDTFEVSGRGELHLTILLENMRREGYELAVGRPRVVFKEI
                     DGVKCEPYEMLSVDVEEEHQGGVMEELGRRRGEMQDMQPDGKGRVRLEYRIPARGLIG
                     FQGEFLTLTRGTGLASHVFDDYSPMAGQMSERRNGVLISQDDGDAVAYALWKLQDRGR
                     MFVSPGDALYEGMVIGIHTRDNDLVVNPIKGKQLTNVRASGTDEAVRLVPPIQLTLES
                     AIEFIADDELVEITPKNIRLRKRYLKEHERKRAARADA"
     gene            3604..4341
                     /locus_tag="PKX01_14325"
     CDS             3604..4341
                     /locus_tag="PKX01_14325"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HMZ77355.1"
                     /translation="MLAIIGGSGLTQLANLEVLRREVVRTPYGEPSGALTFGRLCANP
                     VVFLARHGYGHTIPPHRVNYQANLWALQQAHVTSIISVASVGGIRADLGPGALVVPDQ
                     IIDYTWGRKSTFFEGGDAPVVHVDFTEPYDAGVRQRILAAAVAAGEAVSDGGVYAATQ
                     GPRLETAAEINRLERDGADVVGMTGMPEAVLARELGIPYAAINVVANYAAGRAGSANG
                     ICFDSIEQVLLGAMGRVRRVLDHLCDA"
     gene            4338..4871
                     /gene="def"
                     /locus_tag="PKX01_14330"
     CDS             4338..4871
                     /gene="def"
                     /locus_tag="PKX01_14330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ77356.1"
                     /translation="MIRDVLRMGDPRLLAVARPVEAFDTPELRGLIQDMRDTMTALNG
                     AGIAAPQIGVDLRVVIFGGGGPNPRYPDAEFVPDTVLINPQIELLSDEEEGAWEGCLS
                     VPGLRGWVPRRVHLRYRGVDMEGRPFEREARGFHARVVQHECDHLDGILYPARVRDFS
                     RFGFTDVLFPGVPLADD"
     gene            complement(4896..7493)
                     /locus_tag="PKX01_14335"
     CDS             complement(4896..7493)
                     /locus_tag="PKX01_14335"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HMZ77357.1"
                     /translation="MSQTESPALLSFEQSRELITRIKTRLKSGAETLRAAYEAHPLTE
                     PILKGRCQLVDEVLVELWQIFQLPDSAALVAVGGYGRGELFPCSDVDLLFLLDAPADP
                     ELSSRLEQLIGLIWDIGLDIGHSVRTVEECLEESARDVTVTTNLLEARLLHGNAAMYA
                     DFVRRLHQHVDMGTFFKAKRLEQEQRYTRYQDTPYSLEPNCKESPGGLRDLQVIGWIS
                     RAAGLGTRWRDLARHGLVTEEEATELREVERFLQHVRIRLHHLTGRREDRLLFDHQEK
                     LARRFGYEATGTRRASEVFMQEYYRSAKKVTQLNTILLQNFGAELFPNRYPIAININE
                     RFQCVRELLDVRDEGVFERHPGALLECFLILEQRSELKGMTARTLRALWRGRKLINAD
                     FRRDPAHRALFLRILQQKRGVLHEVRRMNQYGILSFYLPAWRRIVGQMQHDLFHVYTV
                     DQHILQVLRNVRRFMVADHAHEYPLLTRLATSFEKPWLIYIAALFHDIAKGRGGDHSK
                     LGMKDAREFCESHGLDDTDTGLVEWLVEHHLSMSNVAQKHDLSDLSVIQRFAALVGDE
                     RHLTALYILTHADIRGTSPKVWNGWKGKLLEDLFFATQRLLRGVAPQQALGSPKRQDD
                     ARHLLRYYGLRPGTEDALWAQLDTLYFLRHDAEEIAWHARMLYYRVETDQPVVKARLS
                     HVGEGLQVMVYMHDQRDLFMRLCGFFARLGFTIADAKIHTTRHGYALDSFVLLDPGQE
                     LQYRDITNLLEHDLTERLRTQAPADQPTNGRLSREVRHFPITPEVSIRPDDRGKHYIM
                     SVTAADRTGLLFDIADTLAHYSINLHTAKIATLGERVEDTFLISGPGLSQDNLVVKIE
                     SDLLEKLAI"
     gene            complement(7495..8316)
                     /gene="map"
                     /locus_tag="PKX01_14340"
     CDS             complement(7495..8316)
                     /gene="map"
                     /locus_tag="PKX01_14340"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ77358.1"
                     /translation="MSVTIKTPEEIEKMRIAGRLGSEVLDYITPFVKPGVTTGELDKL
                     CHDYMVDVQGCIPAPLNYAPPGYKPYPKSICTSINHQVCHGVPGEKALKKGDIVNLDI
                     TVIKNGYHGDTSRMFIVGGEAAGSILARRLCQITFECLWLGIAQVKPGAHLGDIGHAI
                     QKHAEGHGFSVVREFCGHGIGAKFHEDPQVLHYGKPGTGLELKPGMIFTIEPMINAGK
                     AAISELPDGWTIVTRDRSLSAQWEHMILVTETGVDILTLSEGAPRRPELVAHLFP"
     gene            8615..9367
                     /gene="rpsB"
                     /locus_tag="PKX01_14345"
     CDS             8615..9367
                     /gene="rpsB"
                     /locus_tag="PKX01_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMZ77359.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGQRNKIHIVNLEK
                     TLVKYNEAMDFVRKLAANRGTILFVGTKRQAREIVAEEARRAGMPYVDERWLGGMLTN
                     FKTVKVSIKRLKDMEAMVEDGSIERLSKKEALMASRELEKLQKSLGGIKDLGGLPDAL
                     FVIDVGYHKIAITEANKLGIPVVAVVDTNHTPEGVDFVIPGNDDSSRAIRLYARGVAD
                     AVLEGKNQVVQEIVAAGSDEFVEVEEGNEEQA"
BASE COUNT         1590 a         2792 c         3297 g         1759 t
ORIGIN      
        1 cgaccacgga cgcggctggc aactctgcga gcgcggcgag cagcttcgtc gagtatctgc
       61 acgcgccgac cgcgccgaca ttgacgctca gtacgccgac ggttccgact ctgtccaatg
      121 gcctgattcg ggatacctgg aacgcgtcgg tcgggcccta caacagtggc aacggcgtgg
      181 caccgaccac gatggaggcc agcatcgacg ccctgattgc ggcgggcacc gcgccgaata
      241 catcaacggt gctgacgaac gcggcggtcg cgtcggtggc ggtcggcgtc gcgtccgaga
      301 cccatggcct gatctacctg accggaggga ccgcctacac cttccagatc aagggcgatg
      361 acagcatcct gctcgagatc ggcgggcaga cggtgggcag cgtgacctgg ggaaccggca
      421 gtctggccaa tggcaagttc acgccgacca cgtccggctg gtacacgctg gatctttatc
      481 atgacaacga ggctgggccg ggtaacttca ccgtgcgcgt ctcgacgaat ggttcgtcct
      541 tcgtcaatct ggacacgacc aacttccagc tcgcccagag cgtgcaggat ctcaattcgg
      601 ccggcatccg cacgtcgagc ctgcagggtt ccagcaccac cgaacccaac gacggttact
      661 acacggtgct gccctataac gaggggcatg aggcggcttg gatcccgctg tccaccgtgg
      721 cgggtagcct ggtttccccc ggcgcgtcgg acgtcctgaa catccacatc accggccttg
      781 cgcagaacac ccaacttact gacgggaccc actgggcgac ggccgacgcg agtggcaccg
      841 tggacgtcac gggatggacg atgaatgccc tcagtgccct ggcaccggtg gactattccg
      901 gcaccatgaa tgcccaggtc caggctatcg ccaccgatac gctcaacggc gtatcaacga
      961 cgacgaacaa ggcgctcgcg atcgtggtgg atccgtatgt gacggtggcg ggcacgaccg
     1021 gcacgggtac cgacgacagg attgtcggca cgtctggcgc ggacacgctc aagggcggcg
     1081 aaggcagcga tctgatcatc ggcggagcgg gcagtgacac cctgaccggc ggcgccggtt
     1141 ccgatgtatt ccgctggatg cttgccgaca agggcacgcc tggcctgccg gcccaggaca
     1201 ccatcaccga ctggtcaagt gcggcggtga agaacggtgg cgacgtgctc gatctgcggg
     1261 acctgctcca gggcgagagc cacggggcca gcgggctggg caacctgacc agctacctgc
     1321 acttcgagaa ggtagggacg gcaacggtgg tggatatcag ttcctccggc ggctactccg
     1381 cgggcttcaa ggcgagtgcg acggatcaga ccatcgtcct gtccaacacg gatctgacca
     1441 gtggcggcac cctgaacgat gcgcagatca ttcaggatct gatgaccaag ggcaagctgc
     1501 atacggattg atcgatccgg cgaggctggc ggagtgcacg gcgccggcac aaaacaaaac
     1561 ctccaccgcc tgaggcgcgt ggaggttttg tctttggagg gcggaccaaa tgccttcacg
     1621 ttcgtccggg ccgggacgca tccggcccgg gcgacgtatc aggcgtcggc gcgggcagcg
     1681 cgcttgcgct cgtgttcctt gaggtagcgc ttgcgcaggc ggatgttctt cggcgtgatc
     1741 tccaccagct cgtcgtcggc gatgaactcg atggcggatt ccagggtgag ctggatcggc
     1801 ggcaccaggc gcacggcttc gtcggtgccg gaggcacgca cgttggtgag ctgcttgccc
     1861 ttgatggggt tcaccaccag gtcgttgtcg cgggtgtgga tgccgatgac catgccttcg
     1921 tagagggcgt cgccgggact cacgaacatg cggccgcggt cctgcagttt ccacagggcg
     1981 taggcgacgg cgtcgccgtc gtcctgagag atcagcacgc cgttgcggcg ctcactcatc
     2041 tggccggcca tggggctgta gtcgtcgaag acgtggctgg cgaggccggt gccgcgggtg
     2101 agggtgagga attcgccctg aaagccgatc aggccgcggg ccgggatgcg atattcaagg
     2161 cgcacgcggc ccttgccgtc gggctgcatg tcctgcattt cgccgcggcg gcggccgagt
     2221 tcttccatca cgccaccctg gtgttcctct tccacgtcca cgctgagcat ttcgtagggc
     2281 tcgcacttga cgccgtcgat ctccttgaag accacgcggg gccggccgac ggccagttcg
     2341 tagccttcgc ggcgcatgtt ctcgagcagg atggtgaggt gcagttcgcc gcggccggac
     2401 acttcgaagg tatcggagtc gctggtgaaa ttgacgcgca gggcgacgtt cttgaggagt
     2461 tccttctcca ggcgttcgcg gatctggcgg ctggtgacga acttgccttc acggccagcc
     2521 aggggcgagc tgttgaccat gaagttcatg gtcagggtgg gttcatccac cgcaatgggc
     2581 ggcagcggct caatggactc gggcgcgcac agggtgacgc cgatgttcac ctggtcgatg
     2641 ccggacacca gcacgatgtc gccggcttcg gccgattcgg ccggggcgcg gtcaaggccg
     2701 gtgaaggtga ggatctggct gatcttggcc ttgccccggc tttcgtcacc gtacatgacg
     2761 gccacttcct ggttggggcg gatgcggcca cgcttgatgc gaccgatgcc gagctggccg
     2821 atgtaggtgg agtagtccag ggagcagatc tggagctgca gcggcgcttc cgcatccacg
     2881 tcgggttcgg gcacgtgctg gagaatggcc tcgtagaggg gcttcatgtc ggtgccgggc
     2941 tggtcggcgt cgagcatggc gtagccgttg agggccgagg catacacgac cgggaagtcg
     3001 agctgttctt cggtggcgcc gagcttgtcg aagaggtcga aggtgtggtt gatcacccag
     3061 tccggacggg agccggggcg gtcgatcttg ttgatgacga cgatgggctt gaggccaagg
     3121 gccagcgcct tgcgggtcac gaagcgggtc tggggcatgg ggccttcgac cgcatccacc
     3181 agcagcagca cgccgtccac catggacagg acgcgctcca cttcaccgcc gaagtcggcg
     3241 tggcccgggg tgtcgacgat gttgatgtgg gtgtcgccgt actggatggc gcagttcttg
     3301 gcgaggatgg tgatgccgcg ctccttttcc aggtcgttgc tgtccatcac ccgttcggag
     3361 acctgctggt tgtcgcggaa ggtgccggac tggcgcagga gctggtcgac gagcgtggtc
     3421 ttgccgtggt ccacgtgggc gatgatggcg atgttgcgga tggagcggga catggggttg
     3481 ggttccggaa gtacttgtaa acgcgaaatt ttagcacgcc attgcgcatc gcagcatggc
     3541 cggatgcgca tctgctgcgt gctaagatgc cggccctgag cgaaattcaa ggagtgcacg
     3601 aacatgttgg ccatcatcgg cgggagtgga ctgacgcaac tggcgaacct ggaagtcctg
     3661 cgccgggagg tggtgcgcac gccctacgga gagccttcgg gcgcgctgac cttcggccgc
     3721 ctgtgtgcca atccggtggt ctttctggcg cgccatggct acggccacac gatcccgccg
     3781 caccgggtga attaccaggc caacctgtgg gcgctccagc aggctcacgt cacgtccatc
     3841 atctcggtgg cctcggtggg cggcatacgg gccgatctcg ggcccggcgc gctggtggtg
     3901 ccggaccaga tcatcgatta cacatggggc cgcaagagca ctttctttga agggggtgac
     3961 gcgccggtgg tgcacgtgga tttcaccgag ccctacgatg ccggcgtgcg tcagcgcatc
     4021 cttgcggcgg cggtggcggc gggtgaggcg gtgtccgacg gcggagtcta tgctgcgacc
     4081 caggggccgc gtctcgagac ggcagccgag atcaaccgcc tggaacggga tggggccgac
     4141 gtggtgggca tgaccggcat gccggaggcc gtgctggcgc gggagctggg catcccctac
     4201 gcggccatca acgtggtggc caactacgcc gccgggcggg ccggcagcgc caacgggatc
     4261 tgcttcgaca gcatcgagca ggtgctgctc ggcgccatgg ggcgggtgcg ccgcgtcctc
     4321 gatcatctct gcgacgcatg atccgcgacg tcctgcgcat gggagatccg cgcctgctgg
     4381 cggtggcgcg gccggtggag gcctttgata cgcccgaact gcgaggcctg attcaggaca
     4441 tgcgcgacac catgaccgcg ctcaatggcg ccggcatcgc tgcaccgcaa atcggcgtcg
     4501 atctgagggt ggtgatcttc ggcggtggtg ggccgaatcc gcgttatccc gacgcggagt
     4561 tcgtgccgga cacggtgttg atcaacccgc agatcgagct gctgtcggac gaggaggagg
     4621 gtgcctggga aggttgcctg tcggtgccgg gcctgcgtgg ctgggtgccg cgccgggtgc
     4681 atctgcgcta tcgcggcgtc gacatggaag gtcgaccctt tgagcgggag gcgcggggat
     4741 ttcatgcccg ggtcgtgcag cacgagtgcg accacctcga cggcatcctg tatccggccc
     4801 gggtgcggga tttcagccgc ttcggcttta ccgacgtgct ctttccgggc gtgccgctgg
     4861 cggacgactg acgggccgac gggcggcccg ccggatcaga tcgccagttt ttccagcagg
     4921 tcggattcga tcttcaccac cagattgtcc tggctgaggc cggggccact gatgaggaag
     4981 gtgtcttcca cccgttcgcc gagggtggcg atcttggccg tgtgcaggtt gatgctgtag
     5041 tgggccaggg tgtcggcgat gtcgaagagc agcccggtgc ggtcggccgc ggtcaccgac
     5101 atgatgtagt gcttgccccg gtcgtccgga cggatgctga cttccggcgt gatcgggaag
     5161 tggcgcactt cccgcgacag gcggccgttg gtgggctggt cggcgggcgc ctgagtgcgc
     5221 agacgctcgg tcaggtcgtg ttccagcagg ttggtgatgt cccggtattg cagttcctgg
     5281 ccgggatcga ggaggacgaa gctgtccagc gcatagccgt ggcgggtggt gtggatcttg
     5341 gcgtcggcga tggtgaagcc gaggcgcgcg aagaagccgc acaggcgcat gaagaggtcg
     5401 cgctggtcgt gcatgtagac catgacctgg agtccttcgc ccacgtggct gaggcgtgcc
     5461 ttgaccacgg gttggtccgt ctctacgcgg tagtacagca tgcgtgcgtg ccaggcgatc
     5521 tcttctgcgt cgtgacgcag gaagtagagc gtatcgagct gggcccacaa cgcgtcctcg
     5581 gttccggggc gcaggccgta gtagcgcagc aggtgacggg cgtcgtcctg acgtttcggg
     5641 gagccgagcg cctgttgcgg cgccacgcca cgcagcaggc gctgggtggc gaagaagagg
     5701 tcctccagca gcttgccctt ccagccgttc cacaccttgg ggctggtgcc gcggatgtcc
     5761 gcatgggtga ggatgtacag ggccgtgagg tggcgttcgt cgccgacgag ggcggcgaag
     5821 cgctggatga cgctcaggtc cgaaagatcg tgtttctgcg ccacgttgga catgctcagg
     5881 tggtgctcca cgagccattc caccagaccg gtatccgtgt cgtccaggcc gtgggattcg
     5941 cagaattccc gtgcgtcctt catgcccagc ttggagtggt cgccaccgcg gcccttggcg
     6001 atgtcgtgga acagcgcggc gatgtagatc agccagggct tctcgaagct cgtggcgagg
     6061 cgcgtgagca gggggtattc gtgggcgtgg tcggccacca tgaagcgccg cacgttgcgc
     6121 agcacctgca ggatgtgctg gtcgaccgtg tagacgtgga agaggtcgtg ctgcatctgc
     6181 ccgacgatgc gccgccaggc gggcaggtag aagctgagga tgccgtactg gttcatccgc
     6241 cgcacctcgt ggagcacgcc acgcttctgc tgcaggatgc gcaggaagag cgcgcggtgg
     6301 gccggatcgc gacggaaatc cgcgttgatg agcttgcggc cgcgccacag ggcgcgcagg
     6361 gtgcgggcgg tcatgccctt gagctcggag cgctgctcca ggatcaggaa gcactcgagc
     6421 agcgcgccgg gatgccgttc gaaaacgcct tcgtcgcgca cgtcgagaag ctcgcgcacg
     6481 cactggaagc gttcgttgat gttgatggcg atggggtagc ggttgggaaa cagctctgcg
     6541 ccgaagttct gcagcaggat ggtgttgagc tgggtgacct tcttggcgct gcggtaatac
     6601 tcctgcatga acacttcgga cgcgcggcgc gtaccggtgg cttcgtagcc gaaacgtcgg
     6661 gcgagctttt cctggtggtc gaagagcagg cggtcctcgc gacggcccgt cagatggtgc
     6721 agacggatgc gcacgtgctg gagaaagcgc tccacttcgc gcagttcggt ggcttcctct
     6781 tcggtcacca ggccgtggcg ggccagatcc cgccagcgcg tgcccagacc cgccgcacgg
     6841 ctgatccagc cgatcacctg cagatcgcgc aggccgccgg ggctttcctt gcagttcggt
     6901 tccagtgaat aaggtgtgtc ctggtagcgg gtgtagcgct gctcctgctc caggcgcttt
     6961 gccttgaaga aggtgcccat gtccacatgt tggtgaaggc gcctgacgaa gtcggcatac
     7021 atcgcggcgt tgccatggag caggcgggct tcgaggagat tggtggtgac ggtgacgtca
     7081 cgggcacttt cttcgagaca ttcctcgacg gtgcgaacgc tgtgaccgat gtccagcccg
     7141 atgtcccaga tcagaccgat cagctgttcc agccggctgc tgagctccgg gtctgcgggg
     7201 gcgtccagca ggaacagcag gtcaacgtcg gaacatggga acagctcccc gcggccatag
     7261 ccgcccaccg cgacaagcgc agcgctgtcg ggcagttgaa agatctgcca gagctcgacg
     7321 agcacctcat ccaccagctg gcagcgcccc ttgagaatcg gctcggtgag aggatgggct
     7381 tcataggcgg cacgcagggt ttcggcgccg ctcttgaggc gggtcttgat tcgggtgatg
     7441 agctcgcggc tctgctcaaa gctcagcagc gcgggtgatt cggtctggga catgtcaggg
     7501 aaagaggtgg gccacgagtt ccggccggcg cggtgcgccc tcggacaggg tgaggatgtc
     7561 gacgccggtt tccgtgacca ggatcatgtg ctcccactgg gccgacaggc tgcggtcgcg
     7621 ggtgacgatg gtccagccgt caggcagctc ggagatcgct gccttgccgg cgttgatcat
     7681 cggttcgatg gtgaagatca tgcccggctt cagttccagt ccggtacccg gcttgccgta
     7741 gtggagcacc tggggatcct cgtgaaattt ggcgccgata ccgtggccgc agaattcacg
     7801 caccaccgaa aagccatggc cttcggcgtg tttctggatc gcgtggccga tatcgccgag
     7861 gtgtgcgccg ggcttgacct gggcgatgcc cagccacagg cattcgaagg tgatctggca
     7921 cagccgccgc gccaggatgc tgccggctgc ctcgccgccg acgatgaaca tgcggctggt
     7981 gtcgccgtgg tagccgttct tgatgacggt gatgtccaga ttgacgatgt cacctttctt
     8041 cagcgccttt tctcccggaa cgccgtgaca cacctgatgg ttgatggagg tgcagatcga
     8101 cttgggatag ggcttgtagc ccggcggcgc gtaattgagc ggtgcgggga tgcatccctg
     8161 cacgtccacc atgtagtcat gacacagctt gtcgagttcg ccggtggtga cgccgggctt
     8221 cacgaaaggc gtgatgtaat cgaggacttc ggagccgagg cggcccgcga tgcgcatctt
     8281 ttcgatctct tcgggtgttt ttatggtgac gctcatggcg gtctgcggat tcggattggg
     8341 ggtgcggtag gctagctcaa gcgacgcgtc ggggcaatcg aagcacgggc gtgcgcttcg
     8401 gggcggtaat aaccgcggcg atcttgaggc ttgggcggcc ctcctgatat actccaaagc
     8461 ttgtccggca tccggtcgga caaaattcac acgcccggtt cacaagggtc cgggatcggc
     8521 gcactgcatg tgcgtggatc caggcgttcg ccatgcatgc agatgcggca tggccggccg
     8581 ggcggcggtc aaacctttgt acggagtact tgcaatgtct gtcaccatgc gtcaaatgct
     8641 cgaagcgggc gtccacttcg gccaccaaac ccgtttctgg aacccgaaga tggccccctt
     8701 catcttcggt cagcgcaaca agatccatat cgtcaatctg gaaaagaccc tcgtcaagta
     8761 caacgaggcg atggacttcg tgcgcaagct ggccgccaat cgcggcacca tcctgttcgt
     8821 cggcaccaag cgtcaggccc gcgagatcgt cgccgaggaa gcccgtcgcg ccggcatgcc
     8881 ctacgtggat gagcgctggc tgggcggcat gctcaccaac ttcaagaccg tcaaggtctc
     8941 catcaagcgc ctcaaggaca tggaagccat ggtggaagac ggctccatcg agcgtctgtc
     9001 caagaaggaa gcgctgatgg cctcccgcga gctcgaaaag ctccagaaga gcctgggcgg
     9061 catcaaggat ctgggcggtc tgcccgatgc gctgttcgtc atcgacgtcg gctaccacaa
     9121 gatcgccatc actgaagcca acaagctggg catccccgtt gtggccgtgg tcgataccaa
     9181 ccacacgccc gaaggtgtgg atttcgtgat cccgggtaac gacgactcct cccgcgccat
     9241 ccgcctctac gcccgtggcg tggccgatgc cgtgctggaa ggcaagaacc aggtcgtgca
     9301 ggaaattgtt gccgccggtt ccgatgaatt cgtcgaagtg gaagaaggta acgaagagca
     9361 ggcttaatgc cggcgttcgt gtccctcttt caactgttcg ttaagcgtta agcattgagg
     9421 agctagcatg gcggaaat
//
LOCUS       DAOQPA010000125         9255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_3996_length_9255_cov_14.426087, whole genome shotgun sequence.
ACCESSION   DAOQPA010000125 DAOQPA010000000
VERSION     DAOQPA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3996_length_9255_cov_14.426087"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKX01_14350"
     CDS             complement(<1..1149)
                     /locus_tag="PKX01_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77360.1"
                     /translation="MTAKPAPELASGGVSRTPIGRADLVRALVRCGGQAGAASLERFA
                     SLAGFVRRPVVLLPRVPIAESQPAPPLPLPAETAPAEVAVAPSAVSWYAVVQMTETPP
                     APDGQPDNGPVCLRGVPPLTADDLLPEASAPLLPDSPLLPWARLWPFLKQTLGATTAG
                     PIDVPRVAGLLAEGRVLRHLPRLCRSRWAPEAQVLVDFDLRLEPFRQDFNDLCAGLLR
                     LRGPLGLQILRLLEEPGGAVRPWGQATAPDRPWRPPAPGAPVLLCSDLGMLAGRDSGL
                     HAAWLRFGRLLNARGIRPWVLAPVSPERLSPAVLRHFEVVCFTPDAPLRPRHAPGAPR
                     EAAALAELLAFLAPAVRVEPALLRAAVRHLAPRAARAGVEAAFWNHPDF"
     gene            complement(1127..2098)
                     /locus_tag="PKX01_14355"
     CDS             complement(1127..2098)
                     /locus_tag="PKX01_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ77361.1"
                     /translation="MNSEKPDLPVNVPLDLAPIGSWPATRHVFDPLSADAIAMAWAAQ
                     RPLLVRGEPGTGKSQLARAVAQTLGRVFIAEVVHARTEPQDLLWRFDTVARLGEAQAA
                     AGRLSGPDQPDPLDPQRFLSPGPLWWAFDWERADECFRQSRHRGNRPPQPAGWTPDRG
                     CVLLIDEIDKAESDVPNSLLEVLGNRSFAVPWSNTVVGQASNCALPLVIITTNEEREL
                     PAAFLRRCLVLQYDLPADLVPWLIDRGAIHFPTCAREVLETAANQLVQDRADAREAGV
                     TPPGQAEYLDLLRAVTTLAGTPGEQLELLAHLSHFAYRKFRDGEARA"
     gene            complement(2079..5366)
                     /locus_tag="PKX01_14360"
     CDS             complement(2079..5366)
                     /locus_tag="PKX01_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77362.1"
                     /translation="MARAARQFPREVERFLRDFKQWDGLSEVARRTLTGLGVKLEPFG
                     VPQAVAPQQNDLRGFECLAYGADGASYAKLADLARDQKISKGLLGITLFRVALLTAQA
                     LVDEIGSAVPGSVWSRLFISINVDAEMLHSPVFAKLLDQIPKPPYQVFLEASENLQPE
                     HVGDLRRVLDRHESWLKLALDDSDELAHDTRELLSPRVALVKADGRYVRKLYRDRDKA
                     PDYMLSRLLELRLPGKPFVAEGIEHEDLKRYLKDRWDVSAHGELWMQGYHISVPAPWV
                     DVLEAIHDDPLEPRGFVLPQTLMLRLELNFDSKQHDRIRETPKPEHRPLNVKDESKPH
                     SIRPNPLSENINSTRCTIVDGTEAGPAHRSFIHKELFMAQRIVALTDAWGTRFGGINA
                     FNTGLMKALGTSQGRQFEIVCAVHDIDSAEREDVWTQYSIRVVATGLSTEDVSDTALE
                     KWRTLVGSTGDSPDAFQGTVWLGHDDKSGPLAIALRNRFGGKAILIHHMAHGAYQAFK
                     KGNSVAAHEKENMQRELFSEADLCFAVGPRLRNELEHLLSTVHGAPAVGMLIPGLESP
                     EDYKVKWLDKPSMYFGGFAAGRLTRNDDRIKQGRLAVRAISTVIAEARKTGLIPRLTN
                     APRMCMMGAEPGQEAELRQSMQTWADAQLQIKILPFSEDRTAYYRELSGASFAMMLSW
                     HEGFGLTGWEAISARVPLILGKNSGLWEFLRDEMNGTGLDQCIFPLDIAGNLPSQEGD
                     ENHSEADVSNVAMAIQKLAINAEQAKAKAIRLHDAILEAGWTWARAAKEFVERMNGLP
                     GGGVYPPEVPEPPASAYAPVLDWILAEAACELVKKTCQSIAITLLAMNPPGKATDVVQ
                     LRQGLEAWIEQMPDAVKRIEVLERAVRQAFEPLSPTEHERTALLRHGERLLGLMALSA
                     VKPQALPKGEDLGEELFKLGIGTVVGVELIQAAQSRRTEVVFVWVGKDRKQIRSSRML
                     SVNSLENGWDVQASANEAIKEIVKCKVELAGDQAPTQDQIDEAVYLLTRDGHYFAVSP
                     SLTPNHALLDKEARTVIHARFPDQPIYLFDPAQGHAAHEVFSVSEGRLTGAIRKYLEM
                     LDEFRKA"
     gene            5541..6170
                     /locus_tag="PKX01_14365"
     CDS             5541..6170
                     /locus_tag="PKX01_14365"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HMZ77363.1"
                     /translation="MPHDVHPTLPAALIACHECDLLQTEPRLAAGETARCGRCGAFLA
                     RNPPDSLDRTLALVLTAAVLYALANAFPLVGLEMQGRQVEVTLLEAVRTLWQDGMQAV
                     AALVFVTAQLFPVLELLMIVSVLLPMRLGQAPPALAAFYRLVRSVRPWGMVEVFLLGM
                     LVSLVKLSHMASVILGPAFWAMAALIGVLTLAGRAFDTRLLWQTPEQTP"
     gene            6167..6847
                     /locus_tag="PKX01_14370"
     CDS             6167..6847
                     /locus_tag="PKX01_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HMZ77364.1"
                     /translation="MSAAPPLTALRAGLASCHVCGQLARLPAADAHHARCPRCGAPLH
                     ARKPASIATTWALVLASILLYLPANLLPMMITASFLGSQEDTILSGVIFLWESGSWPL
                     AVVVFFASVMVPLLKILALLYLLISVQRRSRRHPLQRTRLYRVVEFVGRWSMLDIYVI
                     TLLVALVHFQGLATVQAGPAAVAFGAVVVLTMFAALSFDPRLIWDPLADAAPSSTPPS
                     PAVPDRHD"
     gene            6840..8486
                     /locus_tag="PKX01_14375"
     CDS             6840..8486
                     /locus_tag="PKX01_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ77365.1"
                     /translation="MTEPTAPPPPEIPVATVEAPRRFSIPLVWIIPLVAALIGLFLAA
                     RSYYEQGPTITIRFATGEGLEPGKTRIKYKDVDVGQITAVALDEDGRKVIASARVARQ
                     AERLLVDDTRFWVVSARVSGSSVSGLNTLFSGAYVGLDAGKSDEVRRAFDALDAPPVI
                     SMDVPGTAFVLDAPSLNSITVGTPVYYRRIEAGQVTGYALADNGRRLEIRIFVKAPYD
                     RFVTADSRFWNASGVDVKLGADGVQVNTESLTSILAGGLAFLTPESASDEPAPKDTRF
                     RLYNSREEATKQAGQNAQRYLLRFGESVRGLSVGAPLDFRGIAVGEVTAIHADLGTQG
                     SEMGMLVEVAVFPGRMLPRPDAVKPTFIGKDYRDKDFRAYFDKLIAGGLRAQLRSGNL
                     VTGQLYVALDFFPNARPGKVDWAATPPLIPTQRGSLEELQNTLMRLVAKLDRIPVEDI
                     GRDTHKAIVSLGRTVDELEQLIKRMDGLMQGDARSTLADARAAIADARQLLAADAPMQ
                     QDLRASLQELTRAATALRALADTLERTPESLLRGKSEDTP"
     gene            8483..9094
                     /locus_tag="PKX01_14380"
     CDS             8483..9094
                     /locus_tag="PKX01_14380"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HMZ77366.1"
                     /translation="MTRSHHLHRSAPALAALLLSAALAACSSSPPVRHYSLGPLAHSA
                     GDASAAPPDSGAGPSLIVGPVSLPDVIDRPQIVRIVDGVRVEVADTHRWSGNLRNEIA
                     RRLATGIAHNRHLGRVVAWPASSVVQPDYTVPVDIQRLETTAFDHVTLEAVWTLRHAG
                     KDVAGGRFVATEKIEGGTYEGLAAAHGRLVDALAREIGGKVGR"
BASE COUNT         1563 a         3213 c         2932 g         1547 t
ORIGIN      
        1 gaaatcggga tgattccaga acgcagcctc cacgcccgcg cgggcggcgc ggggcgccag
       61 gtgacgcacg gccgcccgca gcagggccgg ttcgacgcgc acggcgggtg ccaggaacgc
      121 cagcagttcg gcaagggcgg ccgcctcgcg gggcgcgccg ggtgcgtgac ggggccgcaa
      181 gggcgcatcc ggcgtgaagc agacgacctc gaagtggcgc aacaccgccg ggctgaggcg
      241 ctcggggctc accggcgcca gcacccacgg ccggattccg cgggcgttga ggaggcgacc
      301 gaaacggagc cacgccgcgt ggagacccga atcgcggccg gccagcatgc cgagatcgct
      361 gcacaggagg actggcgccc ccggcgctgg cggacgccag ggacgatcgg gcgcggtggc
      421 ctgcccccaa ggccggacag caccgccggg ctcctccagc aggcgcagga tctgcaggcc
      481 aagcggtccc cgcaggcgga gcagacccgc acacagatcg ttgaaatcct gacggaaggg
      541 ttccagccgc aggtcgaaat ccaccagaac ctgggcttcc ggtgcccagc ggctgcggca
      601 caggcggggc agatgacgga gcacacgccc ttcggccagc agaccggcga cgcgggggac
      661 gtcgatgggc ccggccgtcg tcgcgcccag cgtctgcttg agaaacggcc acagacgcgc
      721 ccatggcagg agcggactgt cggggagcaa cggcgcggac gcttccggca ggaggtcgtc
      781 cgcagtgagg ggtggcacac cgcgcaggca caccgggccg ttgtccggct gaccgtccgg
      841 ggccgggggc gtctcggtca tctgtacgac ggcataccag ctgacggcag acggcgcgac
      901 ggccacctcg gcgggtgcgg tctctgcagg cagcggcagc ggcggagccg gctgggactc
      961 ggcgatgggc acgcgcggca gcaggacgac cggccggcgt acaaatccgg cgagactggc
     1021 gaagcgctcc agggacgccg ccccggcctg tccgccgcag cggaccagcg ccctgaccag
     1081 atcggcccgg ccaatgggcg tccgggatac gccgcccgac gcgagctcag gcgcgggctt
     1141 cgccgtcacg gaattttcgg taggcaaagt ggctgaggtg cgccagcagc tcaagttgct
     1201 cgcccggcgt tccggccagt gtggtcacgg ctcgcagcag atcaagatat tcggcctgac
     1261 cgggcggcgt aacgccggcc tcgcgggcat cggcgcggtc ctggacgagc tggttcgcgg
     1321 cggtttcgag cacctcgcgg gcacacgtcg ggaaatggat tgcgccccga tcaatcaacc
     1381 agggcaccag atcggcaggc agatcgtact ggaggacaag gcagcggcgc aggaaggcgg
     1441 cgggcaattc ccgctcctcg ttggtggtga tgatcaccag cgggagcgcg cagttcgatg
     1501 cctgccccac cacggtattg ctccagggca ctgcgaagct gcgattgccc aggacttcca
     1561 gcaggctgtt gggaacgtcc gattcggcct tgtcgatctc gtcgatgagc agcacgcagc
     1621 cgcggtccgg tgtccagcca gcgggctgtg gcggccggtt gccacgatga cggctttgcc
     1681 tgaagcattc gtcggcccgc tcccagtcga atgcccacca caagggcccg ggactgagga
     1741 agcgctgggg atcgagggga tccggctgat ccggaccgga cagccgtccg gcggcagcct
     1801 gagcctcgcc gagacgggcc accgtatcga agcgccagag cagatcctgg ggttcggtgc
     1861 gagcatgcac cacctcggcg atgaagaccc gtcccagcgt ttgggccacc gcccgcgcca
     1921 gctggctttt gccggtgccg ggctcgcccc gcaccagcag gggtcgttgc gccgcccagg
     1981 ccatggcgat ggcgtcggcc gaaagcgggt cgaagacgtg ccgggttgcc ggccaggacc
     2041 cgattggcgc caggtccagc ggcacattga cgggcaggtc aggcttttcg gaattcatcc
     2101 agcatctcca gatacttgcg aatggcgccg gtcagccggc cttcgctcac gctgaacact
     2161 tcgtgagcag catgcccctg ggccggatcg aacagataga tcggctgatc cgggaatcgc
     2221 gcgtggatca cggtacgggc ttccttgtcg aggagcgcgt ggttgggcgt caaggacggc
     2281 gacacagcga aataatgccc gtcgcgggtc agcagataaa cggcttcatc aatctgatcc
     2341 tgggtgggcg cctgatcgcc tgcaagttcg accttgcact tgacgatctc cttgatggcc
     2401 tcattggcgg aggcctggac gtcccacccg ttttcaagcg aatttaccga caacatccgg
     2461 ctggaacgga tctgcttgcg atccttgccc acccagacga agaccacttc ggtacgcctg
     2521 ctttgggccg cctggatcaa ctcgacaccc accaccgtcc cgatgcccag cttgaaaagt
     2581 tcctcaccca gatcctcgcc cttggggagc gcctggggtt tgacggcact gagggccatg
     2641 agtcccagca gacgttcgcc atgacgcaac aaggccgtgc gttcgtgttc cgtcgggctc
     2701 aacggttcga atgcctgccg cacggcacgt tccagaactt cgatgcgctt gaccgcatcg
     2761 ggcatctgct cgatccacgc ttccagaccc tgccgcaact gcacgacatc cgtcgcctta
     2821 cccggaggat tcatggccaa aagcgtgatc gctatcgact ggcacgtctt tttcaccaat
     2881 tcgcaggcag cttcagcaag gatccagtcc aggaccggcg catacgcaga cgcagggggc
     2941 tcaggcacct cgggcggata aacgccaccg cccggcagac cgttcatgcg ctcgacgaat
     3001 tccttcgccg cccgtgccca ggtccagcct gcttcgagta tcgcatcgtg cagacggatg
     3061 gccttggcct tggcctgttc ggcattgatc gccagtttct gaatggccat cgccacgtta
     3121 ctcacgtctg cttcgctgtg attctcatca ccttcctgac ttggaagatt tcccgcgata
     3181 tccagcggaa agatgcactg atccagaccg gtgccattca tttcatcccg caggaattcc
     3241 cacagccctg aattttttcc gagaatcaag ggaacacgtg cggagatcgc ctcccagccc
     3301 gtcaggccga agccttcgtg ccacgaaagc atcatggcaa agctggcgcc ggacaattcg
     3361 cgataataag cggtccggtc ttcggaaaaa ggaagaatct tgatctgcaa ttgggcatcc
     3421 gcccatgttt gcatcgactg tcgcagttcg gcctcctgac cgggctcggc acccatcatg
     3481 cacattctcg gcgcattcgt cagacgggga atcaagcccg tcttgcgtgc ctcggcgatg
     3541 accgtagaaa tggcccgcac agccaatcgt ccctgcttga tccgatcgtc gttgcgcgtc
     3601 aggcgccccg cggcgaatcc gccgaaatac atggacggct tgtccagcca tttcaccttg
     3661 taatcttccg gactctcaag cccgggaatc agcataccca cggcaggcgc gccgtgcacc
     3721 gtgctcagga gatgttcgag ttcgttccgg agtcgaggcc ctaccgcgaa gcagagatcg
     3781 gcctccgaga aaagctcgcg ctgcatgttt tccttttcgt gggccgcaac gctgttccct
     3841 ttcttgaatg cctgatacgc accgtgagcc atgtgatgga taaggatggc cttaccgcca
     3901 aaacgattgc gcaatgcgat cgccaatgga ccggatttat cgtcgtgtcc cagccagacc
     3961 gtaccctgaa atgcatccgg ggagtcgcca gtgctaccca caagggtccg ccatttttcc
     4021 agcgccgtat cactgacatc ctcggtggaa agcccagttg ccacgacgcg tatgctgtat
     4081 tgagtccaga cgtcttcacg ttcagcgctg tcaatatcat gtactgcgca gacaatctca
     4141 aattggcgcc cctgggacgt tcccagggct ttcatcaatc cggtattgaa tgcgttgatg
     4201 ccaccgaaac gcgttcccca tgcgtcagtt agcgcaacaa tacgctgggc catgaataac
     4261 tccttatgaa tgaatgaacg gtgagcgggg cctgcctcgg ttccgtcgac gattgtgcag
     4321 cgggtactgt ttatattttc cgaaagcgga ttagggcgaa ttgaatgggg cttagattca
     4381 tctttaacat taagaggtct gtgttccggt ttgggcgttt cgcggatccg atcgtgttgc
     4441 tttgagtcga aatttaattc aagtctcaac atcaacgtct ggggcaacac aaacccccgc
     4501 ggctccagcg gatcatcatg gatcgcctcc aacacatcca cccacggcgc cggcacgctg
     4561 atgtgatacc cctgcatcca caattcccca tgggcgctca cgtcccaacg gtccttcagg
     4621 tagcgtttga gatcctcgtg ctcgatgcct tcggccacga agggtttgcc gggcaggcgc
     4681 agttccagca ggcgtgaaag catgtagtcg ggcgccttgt cccggtcgcg gtagagcttg
     4741 cgcacgtagc gtccgtctgc cttgaccagg gccacccggg gactgaggag ttcgcgggtg
     4801 tcgtgggcca gttcgtcgct gtcgtcgagg gcgagtttca gccaggattc gtggcggtcc
     4861 aggacgcggc gcaaatcgcc cacgtgttcc ggctgaaggt tttcgctggc ttcgagaaag
     4921 acctggtagg gcggcttggg gatctggtcg aggagcttgg cgaagaccgg cgagtggagc
     4981 atttcggcgt ccacgttgat gctgatgaag agccgggacc agacgctgcc gggcacggcg
     5041 ctgccgatct cgtccaccag cgcctgggcc gtgaggaggg ccacgcggaa gagggtgatg
     5101 ccgaggaggc ccttgctgat cttctgatcg cgagcgaggt cggccaattt cgcatacgaa
     5161 gcgccatccg ccccataggc caggcattca aagccgcgca ggtcgttctg ttgcggcgca
     5221 acggcctggg gcacgccaaa gggttcgagc ttgacgccca aacctgtgag cgtccggcgg
     5281 gccacttccg acagcccgtc ccactgtttg aaatcccgca ggaagcgttc gacttcccgg
     5341 gggaactgac gtgctgcgcg tgccatgagc atctccctgt tctacatacc aaaatcatgc
     5401 atgaatcccc atggtctgag aagggaaatg acgcctgcag gtggatcgga cgattcaccg
     5461 caccgatccg cctcgccccc cttgcacccc ctccccccgc accgcacaat gcggcgtcat
     5521 accttttcct gccgccgtcc atgccccacg acgtgcatcc gaccctgcct gctgcgctga
     5581 ttgcgtgtca tgagtgcgat ctgctccaga ccgagccgcg gctggcggcg ggggagacgg
     5641 cgcggtgtgg gcggtgtggg gcgtttctgg cgcgcaatcc gccggacagc ctggatcgca
     5701 cgctggcgct ggtgctcact gcggcggtgc tgtacgcgct ggccaatgcc tttccgctgg
     5761 tggggctgga gatgcagggg cggcaggtgg aggtgacgct gctggaggcg gtgcgcaccc
     5821 tgtggcagga cgggatgcag gcggtggcgg cgctggtctt cgtcacggcc cagctctttc
     5881 cggtgctgga gctgctgatg atcgtgtcgg tgctgctgcc catgcgcctg gggcaggcgc
     5941 cgccggcgtt ggcggccttc taccggctgg tgcgctcggt gcgcccgtgg ggcatggtgg
     6001 aggtcttcct gctgggcatg ctggtgtcgc tggtgaagct ctcccacatg gccagcgtga
     6061 tcctgggccc ggcgttctgg gcgatggcgg cgctgatcgg ggtgctcacc ctggccgggc
     6121 gggcctttga cacgcgcctg ctgtggcaga cgccggagca gacaccgtga gcgcggcgcc
     6181 gcccctcacc gccctgcgcg ccgggctggc cagctgtcac gtgtgcggcc agctcgcgcg
     6241 cctgccggcc gcggacgccc accacgcccg ttgcccgcgc tgcggtgcgc cgctgcatgc
     6301 gcgcaagccc gccagcatcg ccaccacctg ggcgctggtg ctggcctcca tcctgctgta
     6361 tctgccggcc aacctgctgc ccatgatgat caccgcgtcc ttcctgggca gccaggaaga
     6421 cacgatcctg agcggcgtga tctttctgtg ggaatccggc tcgtggccgc tggcggtggt
     6481 ggtgttcttc gccagcgtga tggtgcccct gctcaagatc ctggccctgc tctatctgct
     6541 gatcagcgtg cagcgccgct cgcgccgcca tccgctgcaa cgcacgcgcc tgtatcgcgt
     6601 ggtggaattc gtcgggcgct ggtccatgct ggacatctac gtgatcaccc tgctggtggc
     6661 gctggtgcat ttccagggcc tcgccacggt gcaggccggc cccgccgcag tggcctttgg
     6721 cgcggtggtg gtgctcacca tgttcgccgc cctgagcttc gacccgcgcc tgatctggga
     6781 tcccctcgcc gacgcggcgc cgtcctccac accgccttcc cccgccgtgc ccgaccgcca
     6841 tgactgaacc caccgcgccg ccgcctcccg agatccccgt ggccaccgtc gaggcgccgc
     6901 gccgcttcag cattccgctg gtgtggatca tcccgctggt ggctgcgctg atcggtctct
     6961 tcctcgccgc ccgcagctat tacgaacaag gccccaccat caccatccgc tttgccaccg
     7021 gcgaaggcct ggagcccggc aagacgcgca tcaagtacaa ggacgtggac gtgggccaga
     7081 tcaccgccgt ggccctggac gaagacggcc gcaaggtgat cgccagcgcg cgtgtggcgc
     7141 gtcaggccga acgtctgctg gtggacgaca cgcgcttctg ggtggtcagc gcccgcgtct
     7201 ccggcagctc cgtgtccggc ctcaacaccc tcttctccgg cgcctacgtg gggctggacg
     7261 cgggtaaatc cgacgaagtg cgccgcgcct tcgacgccct cgacgcgccg ccggtgatca
     7321 gcatggacgt gcccggcacc gccttcgtgc tcgacgcccc cagcctcaac tccatcaccg
     7381 tgggcacccc cgtgtattac cggcgcatcg aggccggcca ggtcaccggc tacgccctcg
     7441 ccgacaacgg ccgccggctg gagatccgca tcttcgtcaa ggcgccctac gaccgcttcg
     7501 tcaccgccga cagccgcttc tggaacgcca gcggcgtgga cgtgaagctc ggcgccgacg
     7561 gcgtgcaggt gaacaccgaa tccctcacct ccatcctcgc cggcggcctc gccttcctca
     7621 cgccggagag cgccagcgac gagcccgcgc ccaaggacac ccgcttccgc ctctacaaca
     7681 gccgcgaaga agccaccaag caggccggcc agaacgccca gcgctacctg ctgcgctttg
     7741 gcgaatcggt gcgcggcctg tcggtgggcg cgccgctgga tttccgcggc atcgccgtgg
     7801 gcgaagtcac cgccatccac gccgacctgg gcacccaggg cagcgagatg ggcatgctcg
     7861 tggaagtggc cgtcttcccc ggccgcatgc tgccccgccc ggacgccgtc aaacccacct
     7921 tcatcggcaa ggactaccgc gacaaggact tccgcgccta cttcgacaag ctcatcgccg
     7981 gcggcctgcg cgcccagctg cgcagcggca acctcgtcac cggccagctc tacgtggccc
     8041 tggacttctt ccccaacgcc cgccccggca aggtggactg ggccgccaca ccgcccctca
     8101 tccccaccca gcgcggcagc ctggaagaac tccagaacac cctcatgcgc ctcgtcgcca
     8161 agctcgaccg catccccgtg gaagacatcg gccgcgacac ccacaaagcc atcgtcagcc
     8221 tcggccgcac cgtggacgag ctcgaacagc tcattaagcg catggacggc ctgatgcagg
     8281 gcgacgcccg cagcaccctg gccgacgccc gcgccgccat cgccgacgca cgccagctcc
     8341 tcgccgccga cgccccgatg cagcaggatc tgcgcgcctc cctgcaggag ctcacccgcg
     8401 ccgccacggc cctgcgcgcc ctcgccgaca ccctcgaacg cacacccgaa tccctgctcc
     8461 gcggcaaatc cgaggacacg ccatgacccg ctcccaccac ctgcaccgca gcgcccccgc
     8521 cctcgccgcc cttctcctga gcgccgccct cgccgcctgc agcagctcgc cccccgtccg
     8581 ccactacagc ctcggccccc tcgcccacag cgccggcgac gcctccgccg caccaccgga
     8641 cagcggcgcc ggccccagcc tcatcgtcgg ccccgtcagc ctgccggacg tcatcgaccg
     8701 cccccagatc gtccgcatcg tggacggcgt gcgcgtcgaa gtggccgaca cccaccgctg
     8761 gagcggaaac ctgcgcaacg aaatcgcccg ccgcctcgcc accggcatcg cccacaaccg
     8821 ccacctcggc cgcgtcgtcg cctggcccgc cagcagcgtc gtccagcccg actacaccgt
     8881 ccccgtagac atccagcgcc tcgaaaccac cgccttcgac cacgtcaccc tcgaagccgt
     8941 ctggaccctc cgccacgccg gcaaagacgt agccggcgga cgcttcgtcg cgacagagaa
     9001 gatcgaaggc gggacctacg aaggcctcgc cgcagcccat ggacggctgg tggatgcgct
     9061 ggcgcgggag atcgggggga aggtggggcg gtgagggatg cggccggctg aaggggcgta
     9121 gcgctgaccg ggaaatcctc agaaaaggct ggacgtcacc ttccctgaac gatcagacga
     9181 agttgagcca tgacctcttg ccctacccag aagagcttcg agtacgggct ttggtcggct
     9241 tcgacggcgg caaag
//
LOCUS       DAOQPA010000126         9137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4087_length_9137_cov_14.587536, whole genome shotgun sequence.
ACCESSION   DAOQPA010000126 DAOQPA010000000
VERSION     DAOQPA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_9137_cov_14.587536"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..620
                     /gene="nikR"
                     /locus_tag="PKX01_14385"
     CDS             120..620
                     /gene="nikR"
                     /locus_tag="PKX01_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HMZ77367.1"
                     /translation="MERFTISLDEDLARAFDALIAARGYSNRSEAVRDILRAHLEANR
                     VAREEAPHCIANLSYVYNHHERELAERVTQDQHAHHDLCVATLHAHLDHDHCMESVIL
                     RGPTADVRAFAESLIARPGVRHGALNLVTAELHSAAHRHGERADHAHPHPHEGGVSHI
                     HYRPRS"
     gene            624..1982
                     /locus_tag="PKX01_14390"
     CDS             624..1982
                     /locus_tag="PKX01_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77368.1"
                     /translation="MSKPERPLPDSAALLAAAPEGLGEGAWIDVIQKMDEVYSDLLQY
                     EVVLEEKNAKLEESQGFILSVLTSISDILVVCDRSGRIEDVNRSLEQFTGRMLAELRG
                     QSFFDLLADPAAAEAARGLLALRGDAPVHDCELALRAADGSAVPVSLNCTPRYSSVGK
                     SLGMVITGRPVGELRRAYSALRQAHDDLKRTQQQLLHSEKMASLGRLVAGVAHELNNP
                     ISFILGNTHALRRYAQRLEEYLAAVHGGVGAEALADLRERLRIDRILKDLEPLIDGTI
                     EGAERTRDIVDGLKRFSALDREENARFDLAAVIDRSVTWVSKAAPERFRVTVHLPPGL
                     QVTGSAGQMQQVVMNLVQNACDATAGQHPALLEIRGQIAAAGARREVVLDFHDNGSGI
                     EDRHLPHIFDPFFTTKPVGSGTGLGLSISYGIVERHGGRLTAGNHPRGGACFTLRLPL
                     AD"
     gene            2146..2976
                     /locus_tag="PKX01_14395"
     CDS             2146..2976
                     /locus_tag="PKX01_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HMZ77369.1"
                     /translation="MESLPLDSPSLVALVFLLGLKHGFDADHLATIDGLTRFNARSRP
                     RLARLCGTLFSLGHGAVVLAVAVGASLLSRGWQAPEWLELSGAGISILFLLGLGVLNL
                     GAVLAAPRDAVVRPLGLKGRWLGRLAEVSHPAGVALVGALFALSFDTLSQAALFAVAG
                     GSGAGGGALRAAGLAGCFVLGMLVADGANGFWIARLIARADGVARIASRVMALAVAGM
                     SLLVAAYGLARLLSPRIEAWGEGKELLLGAVVVTVMAASFVVGLRLARIQGAPAAARG
                     "
     gene            3208..4089
                     /locus_tag="PKX01_14400"
     CDS             3208..4089
                     /locus_tag="PKX01_14400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMZ77370.1"
                     /translation="MHDTDTVTDPDFDSLVPGQSVGRRTFVGVALAAGFALSVRPALG
                     QDVIHTPADGLLAGEVRIVAPDGVALAAYRARPAGDGPVPVVLVVQEIFGVHEYIRDV
                     CRRLARAGYLAIAPNLFQRQGDPSALASVAEIQAGIVAKVPDAQVLGDLEACAEWAQT
                     QGGDGRLGITGFCWGGRIAWLFAARHAGLKAAVAWYGRLDGTPSANTPTHPLDVVEAL
                     QAPVLGLYGAKDQGIPVSDVEAMRERLAAAGKRGELVLYPDAGHAFHADYRPSYRAAE
                     AADGWARLLAWFRANGL"
     gene            complement(4120..4782)
                     /locus_tag="PKX01_14405"
     CDS             complement(4120..4782)
                     /locus_tag="PKX01_14405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ77371.1"
                     /translation="MELVLFDLDNTLLAGDSDFEWAQFLIAKGVLDKEVYEARNLAFY
                     EQYKAGTLDIYEFLDFQLLPLARHSREQLDLWHREFMDSRIRPMMTPAARALVRRHLD
                     SRAVVAIVTATNSFVTGPIGRAFEIPHLVATVPAQEHGVFTGKPRGTPAFKAGKIERV
                     ENWLEALGLHWGSFRRSWFYSDSHNDLPLLEKVSDPVAVDPDDTLRAHATARGWNILS
                     LR"
     gene            complement(4789..5472)
                     /gene="hda"
                     /locus_tag="PKX01_14410"
     CDS             complement(4789..5472)
                     /gene="hda"
                     /locus_tag="PKX01_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HMZ77372.1"
                     /translation="MKQLTLDLLADAPPLLDSFVPGANADLLAALFECVPVADTPQHL
                     YLWGDAGSGRSHLLRGVVTAAAQAGRPACYLRAADVVDTLPEAPALLLAVDDVERLGA
                     DAQIALFRACNAARGQRMTLVTSGAVPPRDLPLREDLRTRIGQSLIFEVKALGDDERA
                     AILAAHADARGMRLEADIVQYLLRHAPRDLKSLLRTVDALDAASLERKRAITLPLLRD
                     VLQHVPPTA"
     gene            5706..6740
                     /gene="purM"
                     /locus_tag="PKX01_14415"
     CDS             5706..6740
                     /gene="purM"
                     /locus_tag="PKX01_14415"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMZ77373.1"
                     /translation="MSSLTYRDAGVDIDAGDALVDRIKPFAKRTMRPEVLGGIGGFGA
                     LFEISRKFKEPVLVSGTDGVGTKLKLAFQLNKHDTVGQDLVAMSVNDILVQGAEPLFF
                     LDYFACGKLDVDTAATVVEGIARGCELAGCALIGGETAEMPSMYPDGEYDLAGFAVGA
                     VEKSQIIDGSTIRPGDVVLGLASSGAHSNGYSLIRKIIELTQPNLDGDFHGQTLKDVI
                     LAPTRIYVKSLLALMAQRPGLVKGMAHITGGGLLENVPRVLADHLTAVLRQDAWEMPY
                     LFQWLKGAGNVEAQEMYRVFNCGIGMVVIVAAEDADAAAAQLRSTGETVYTLGTIEAR
                     GEGQAQTVVV"
     gene            complement(6913..7545)
                     /locus_tag="PKX01_14420"
     CDS             complement(6913..7545)
                     /locus_tag="PKX01_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HMZ77374.1"
                     /translation="MYLPPLFREDDPARCAELINAHPLATLISQGPGGLQANLVPVLF
                     YPDEGPHGTLRAHLARPNPQLAELAAQPDCLVVFRGPEAYITPAWYPSKRTTGKVVPT
                     WNFATVHAWGPARLIDDPAWLLRLVTDLTDHQEAPRATPWRVDDAPADYISAQLKAIV
                     GLEIPVQRLEGKWKMSQNRSPADRQGVVDGLWDAGEHAVAEAVAQRMRGE"
     gene            complement(7610..8041)
                     /locus_tag="PKX01_14425"
     CDS             complement(7610..8041)
                     /locus_tag="PKX01_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HMZ77375.1"
                     /translation="MFDPSRDQARQFFLDAWRKYRNRDVLTQLETLAADLVAHHPEYH
                     PLLEDADAVHKDFSPEDGQINPFLHLSLHLAIEEQLSINQPPGIRDAFEQCQARHGDR
                     HAALHDVLECLGETLWRAQRDKAPLDGRAYVDCVRKRGGIS"
     gene            complement(8100..8732)
                     /gene="nth"
                     /locus_tag="PKX01_14430"
     CDS             complement(8100..8732)
                     /gene="nth"
                     /locus_tag="PKX01_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMZ77376.1"
                     /translation="MRLAEIAEAFRRLATANPHPTTELEYASPYQLLVAVVLSAQATD
                     KGVNLATRRLFALAPTPEAMVSLGEDAIAEQIKTIGLFRNKARNTLLLSQQLLERHGG
                     EVPHDRAALEALPGVGRKTANVVLNTVFGDPTMAVDTHIFRLANRTGLAPGKDVLEVE
                     KRLLRRIPKAYLRDAHHWLILHGRYVCVARTPKCGECLLRDLCRFPDKTA"
     gene            complement(8745..>9137)
                     /locus_tag="PKX01_14435"
     CDS             complement(8745..>9137)
                     /locus_tag="PKX01_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HMZ77377.1"
                     /translation="VLGIFIPLIVTNCIVLARVEAFAAKNEPLGSTLDGIAQGAGLTL
                     VLVVLGALREFVGSGTLLSGIEMIIPGASPIVLFGDDYPGFLVAILPPGAFFALGSLV
                     AGRNWLEARKAARARQAPPSIDTPVEAG"
BASE COUNT         1234 a         2941 c         3556 g         1406 t
ORIGIN      
        1 ggggcgcgac agggtggggg gacgacgcgg caaggggcgc actgcccacg agggccggcg
       61 attgctcccc gcatgccccg cgggcagaat gcctgctccg tcctcccccg gagcgccccg
      121 tggaacgctt tacgatctcc ctcgacgaag acctggcccg cgcctttgat gcgctgatcg
      181 cggcgcgggg ctattccaac cgctcggagg cggtgcgcga catcctgcgc gcccacctgg
      241 aggccaaccg ggtggcgcgg gaggaggcgc cccattgcat cgccaacctg tcttacgtct
      301 acaaccacca cgagcgggag ctggcggagc gggtcaccca ggaccagcat gcccaccacg
      361 acctgtgcgt ggccaccctg cacgcccacc tggaccacga tcactgcatg gagagcgtga
      421 tcctgcgcgg ccccacggcc gacgtgcggg cctttgccga aagcctcatc gcgcggcccg
      481 gcgtgcgtca cggggccctc aacctggtga cggcggagct gcacagcgcg gcccatcgtc
      541 acggcgagag ggccgaccac gcgcatcccc acccccacga gggaggggtg tcccacatcc
      601 actaccgtcc ccgttcgtaa gccatgagca agcccgagcg accgctgccg gattccgccg
      661 ccctgctcgc cgccgcgccg gaaggcctgg gggagggggc gtggatcgac gtgatccaga
      721 agatggacga ggtgtattcc gacctgctcc agtacgaggt ggtgctggaa gagaagaacg
      781 ccaagctgga ggagagccag ggcttcatcc tgtcggtgct cacctccatt tcggacatcc
      841 tggtggtgtg cgaccgcagc gggcgcatcg aggacgtgaa ccgctccctc gaacaattca
      901 ccggccggat gctggccgag ttgcgcggtc agtccttctt cgatctgctc gccgacccgg
      961 ccgcggcgga ggcggcgcgc ggcctgctgg ccctgcgcgg cgacgcgccg gtgcacgact
     1021 gcgagctggc cctgcgggcc gccgacggca gcgccgtgcc cgtatccctc aactgcacgc
     1081 cgcgctattc gtcggtgggc aagtccctcg gcatggtgat caccggccgg ccggtgggcg
     1141 agctgcggcg cgcctattcg gccctgcgcc aggcccacga cgacctcaag cgcacccagc
     1201 agcagctcct ccattccgag aagatggctt ccctcgggcg tctggtggcg ggcgtggccc
     1261 acgagctcaa caaccccatc agcttcatcc tcggcaacac ccacgccctg cgccgctacg
     1321 cccagcgtct ggaggaatac ctggccgcgg tgcatggcgg cgtcggggcg gaggccctgg
     1381 cagacctgcg ggagcgcctg cgcatcgacc gcatcctgaa ggacctggag cccctcatcg
     1441 acggcaccat cgaaggggcg gagcgcaccc gcgacatcgt ggacggcctc aagcgcttct
     1501 ccgcgctgga ccgggaggag aacgcccgct tcgatctggc ggcggtgatc gatcgctccg
     1561 tcacctgggt aagcaaggcc gcaccggaac gcttccgggt gactgtgcac ctgccgccgg
     1621 ggctgcaggt gacgggctcc gcggggcaga tgcagcaggt ggtgatgaac ctggtgcaga
     1681 acgcctgcga cgccaccgcc gggcagcacc cggcgctgct ggagatccgc ggccagatcg
     1741 cggcggcggg cgcgcgtcgc gaagtcgtgc tggactttca cgacaacggc agcggcatcg
     1801 aggaccgcca cctgcctcac atcttcgatc ccttcttcac caccaagccg gtgggcagcg
     1861 gcaccggcct gggtttgtcc atcagctacg gcatcgtgga gcgccacggc gggcgcctga
     1921 cggcgggcaa tcatccccgc ggcggcgcat gcttcaccct gcgcctgccc ctggcggact
     1981 gacgcgccgc aggtggggcg gtcgtcgtcc tgccggtgtg gcggggcggg cggaaaggga
     2041 cttgccggcg ccgcgcaccc attgcccgtc gcgacgggct tcattccgtg gcatgcttct
     2101 tgtaagcacc ttcagagttt tttcctacgc cgcggagccc gtcccatgga aagcctgccc
     2161 ctcgacagcc cgagcctcgt cgccctggtg ttcctgctcg ggctcaagca tggcttcgac
     2221 gcggaccacc tggccaccat cgacggcctg acgcgcttca atgcccgtag ccggccgcgg
     2281 ctggcacgcc tctgcggcac cctgttctcc ctgggccacg gcgccgtggt gctggcggtg
     2341 gcggtggggg cgagcctgct gtcccgcggc tggcaggcgc cggaatggct ggagctgtcc
     2401 ggcgccggca tctccatcct gttcctgctc gggctgggcg tgctcaatct cggtgcggtg
     2461 ctggccgcgc cccgggatgc ggtggtgcgt ccactaggcc tgaaagggcg ctggctcggc
     2521 cgcctcgccg aggtcagtca tcccgccggc gtggccctgg tgggcgcgct gttcgccctg
     2581 tccttcgaca ccctgagcca ggcggccctg ttcgctgtgg cgggcggcag cggcgccggt
     2641 ggtggcgccc tgcgcgcggc cgggctggcg gggtgcttcg tgttgggcat gctggtggcc
     2701 gacggcgcca atggcttctg gatcgcccgc ctgatcgccc gcgcggacgg tgtggcgcgt
     2761 attgcctcgc gggtgatggc cctggcggtg gcgggcatga gcctgctggt ggcggcctac
     2821 ggcctggccc gcctgctctc gccgcgcatc gaggcctggg gtgagggcaa agagttgctg
     2881 ctgggcgccg tcgtggtgac ggtgatggcg gcgagcttcg tcgtcggtct gcgcctggcg
     2941 cgcatccagg gcgccccggc cgcggcgcgg ggttgacgca gggcggcgga tcgcggtttt
     3001 cgcgatgtcc gccagcggcc gtttccccgc gcggtctgcg cggtgcgggc gccggcgcgg
     3061 cgcagtccgg ttcgccggtc tgcgggatgt gtcgatgggg ggccggttcg cctgccgtgc
     3121 cgtcggtgac gtccccgttc cccgcggtct cgggcatcac catttgcgcg tatgctggcg
     3181 accgacccca tcctccagga gcgcgccatg cacgacaccg acaccgtgac cgatcccgat
     3241 tttgacagcc tcgtgcccgg ccagtccgtg ggccggcgga ccttcgtcgg ggtcgccctg
     3301 gcggcgggct ttgccctgtc ggtgcgtccg gctctgggcc aggacgtgat ccacacgccg
     3361 gcggatggcc tgctggcggg cgaggtgcgc atcgtggcgc cggatggcgt ggcgctggcg
     3421 gcctaccggg cgcgcccggc gggggatggc cccgtgccgg tggtgctggt ggtgcaggag
     3481 atcttcggcg tccatgagta catccgcgac gtctgccggc gcttggcgcg ggcgggttat
     3541 ctggccatcg cgcccaacct gttccagcgc cagggcgatc cgtccgcgct ggccagcgtg
     3601 gcggagatcc aggcgggcat cgtggcgaag gtgccggacg cccaggtgct gggcgatctg
     3661 gaggcctgcg ccgagtgggc gcagacccag ggcggcgacg gccggctggg catcaccggc
     3721 ttctgctggg gcgggcgcat cgcctggctg ttcgcagccc ggcacgccgg cctgaaggcg
     3781 gcggtggcct ggtacgggcg gctcgacggc acgccgtcgg cgaacacgcc cacccatccg
     3841 ctcgacgtgg tggaggcgct gcaggcgccg gtgctgggcc tctacggggc caaggaccag
     3901 ggcatcccgg tgagcgatgt ggaggcgatg cgcgagcgtc tggcggcggc gggcaagcgg
     3961 ggcgagctgg tgctttaccc ggacgcgggc cacgccttcc acgcggacta ccggcccagc
     4021 tatcgggccg cggaggcggc ggacggctgg gcgcggctgc tggcgtggtt ccgcgccaac
     4081 ggtctgtgac ggggcgcccg gaccgccgcc ggggccccgt cagcgcaggc tgaggatgtt
     4141 ccagccgcgg gcggtggcgt gggcgcgcag ggtgtcgtcc ggatccacgg ccaccgggtc
     4201 ggagaccttc tccagcaggg gcaggtcgtt gtgggagtcg ctgtagaacc agctgcggcg
     4261 gaagctgccc cagtgcaggc cgagggcttc cagccagttc tccacgcgtt cgatcttgcc
     4321 cgccttgaaa gccggcgtgc cgcggggctt gccggtgaag acgccgtgct cctgggccgg
     4381 cacggtggcc accaggtggg gaatctcgaa ggcgcggccg atggggccgg tgacgaagct
     4441 gttggtggcg gtgacgatgg ccaccaccgc gcgggagtcc aggtggcgcc gcaccagggc
     4501 gcgggcggcg ggcgtcatca tggggcggat gcggctgtcc atgaattccc ggtgccacag
     4561 gtcgagctgc tcgcggctgt ggcgggcgag gggcaggagc tggaagtcca ggaattcgta
     4621 gatgtccagg gtgccggcct tgtactgctc gtagaaggcc agattgcggg cttcgtagac
     4681 ttccttgtcg agcacgccct tggcgatgag gaattgggcc cactcgaaat ccgagtcgcc
     4741 ggcgagcagg gtgttgtcca ggtcgaagag tacgagttcc atggcggttc aggcggtcgg
     4801 ggggacgtgc tgcaggacgt cccgcagcag gggcagggtg atggcgcgct tgcgctccag
     4861 ggacgcggcg tcgagggcgt ccacggtacg cagcaggctc ttgagatccc gcggcgcgtg
     4921 gcgcagcagg tactggacga tgtccgcctc caggcgcatg ccccgcgcgt cggcgtgggc
     4981 ggcgaggatg gcggcgcgct cgtcgtcgcc gagggccttg acctcgaaga tcaggctctg
     5041 gccgatgcgg gtgcgcaggt cttcccgcag gggcagatcc cgcggcggca cggcgccgct
     5101 ggtgacgagg gtcatgcgct ggccgcgggc ggcgttgcag gcgcggaaca gggcgatctg
     5161 ggcgtccgcg ccgaggcgct ccacgtcatc caccgccagc agcagcgccg gggcttccgg
     5221 cagcgtgtcg accacgtcgg cggcgcgcag atagcaggcg ggtcggccgg cctgggcggc
     5281 ggcggtgacc acgccccgca gcaggtggct gcgcccgctg ccggcgtcgc cccacaggta
     5341 gaggtgctgg ggcgtgtcgg ccaccggcac gcattcgaac agggcggcga ggaggtcggc
     5401 gttggcgccg ggcacaaagc tgtccagcag gggcggggcg tcggcgagga gatcgagggt
     5461 gagctgcttc acggggcgcg gcggggggcc ggggcgtcgg gcggtggtga gcgacgcggt
     5521 cgggtcacaa ttcagctaaa atccgggttt gcgcgcgtgg atcggggccc gggaagtctt
     5581 cccgccggcc ggaacccgcg caaggcccgt taatgtagcc ggtcgcggcg gggcgctcaa
     5641 ccgaatagcg tcccgaaagt gcctcaaccc cctgccgccg gccgttccag agaagatcac
     5701 agcccatgag ctcccttact tatcgcgatg ccggtgtgga tatcgacgcc ggcgatgccc
     5761 tggtggaccg catcaagccc tttgccaagc gcaccatgcg ccccgaggtg ctgggcggca
     5821 tcggcggttt cggcgccctc ttcgagatct ccaggaagtt caaggagccg gtgctggtgt
     5881 ccggcaccga cggcgtgggc accaagctca agctggcctt ccagctcaac aagcacgaca
     5941 ccgtgggcca ggatctggtg gccatgagcg tcaacgacat cctggtgcag ggcgccgagc
     6001 ccctgttctt cctggactac ttcgcctgcg gcaagctgga cgtggacacc gctgccaccg
     6061 tggtggaagg catcgcccgc ggctgcgagc tggccggctg cgccctcatc ggcggcgaga
     6121 ccgccgagat gcccagcatg tacccggacg gcgaatacga cctggccggc ttcgccgtcg
     6181 gcgccgtcga gaagtcccag atcatcgacg gctccaccat ccgtcccggc gacgtggtgc
     6241 tgggcctcgc ctccagcggc gcccactcca acggctactc cctgatccgc aagatcatcg
     6301 agctcaccca gcccaacctg gacggcgact tccacggcca gaccctcaag gacgtgatcc
     6361 tcgccccgac ccgcatctac gtgaaatccc tgctggccct gatggcccag cgccccggcc
     6421 tggtgaaggg catggcccac attaccggcg gcggcctcct cgagaacgtg ccccgcgtcc
     6481 tcgccgacca cctgaccgcc gtcctccgcc aggacgcctg ggagatgccc tacctcttcc
     6541 agtggctcaa gggcgccggc aacgtggagg ctcaggagat gtaccgcgtc ttcaactgcg
     6601 gcatcggcat ggtggtgatc gtcgccgccg aagacgccga cgcggcggct gcgcaactgc
     6661 gttccacggg ggagacggtg tacaccctcg gcaccatcga agcccgcggg gaaggtcagg
     6721 cgcagacggt ggtggtgtaa ggcggtagcg ctgacgcggc ggacgccgct ttcggtagtg
     6781 aatctaacgg gcctgcgggc ccgtttttgt ggcccgttga tctgcagtga agaggggtag
     6841 ggatgcggtg cgtgttgccc ggcccctgta cccccgacgt cgccgacctg ggaacggtgc
     6901 cggcgatgcg cgtcactcgc cccgcatgcg ctgggcgacg gcctcggcca ccgcgtgttc
     6961 gccggcgtcc cacaggccgt ccaccacgcc ctggcggtcg gcggggctgc ggttctggct
     7021 catcttccac ttgccttcga gacgctggac ggggatttcg aggccgacga tggccttgag
     7081 ctgggcgctg atgtagtcgg cgggggcgtc gtccacgcgc cagggggtgg cgcggggggc
     7141 ttcctgatgg tcggtgaggt cggtgacgag gcgcagcagc caggcggggt cgtcgatgag
     7201 gcgggcgggg ccccaggcgt ggacggtggc gaagttccag gtggggacga ccttgccggt
     7261 ggtgcgcttg ctggggtacc aggcgggggt gatgtaggcc tcggggccgc ggaagacgac
     7321 gaggcagtcg ggctgggcgg cgagctcggc gagctggggg ttggggcggg ccaggtgggc
     7381 gcgcagggtg ccgtgggggc cttcgtccgg gtagaagagg acgggcacga ggttggcctg
     7441 caggccgccg ggcccctggc tgatgagggt ggcgaggggg tgggcgttaa tcagctcggc
     7501 gcagcgggcg gggtcgtctt cgcggaacag gggcggcagg tacatggcgg gactcgggcg
     7561 ggagaggcgg tgttgcgcgg ccagtgtagc aggccgggcg gggtggccgt cagctgatgc
     7621 cgccgcgctt gcgcacgcag tccacgtagg cgcggccgtc caggggggcc ttgtcgcgct
     7681 gggcgcgcca gagggtttcg ccgaggcatt cgaggacgtc gtggagggcg gcgtggcggt
     7741 cgccgtggcg ggcctggcac tgctcgaagg cgtcgcggat gccggggggc tggttgatgg
     7801 agagctgctc ctcgatggcc aggtgcaggg agaggtgcag gaaggggttg atctggccgt
     7861 cctcggggct gaagtccttg tgcacggcgt ccgcgtcttc cagcagggga tggtattccg
     7921 ggtggtgggc gacgaggtcg gcggcgaggg tctcgagctg ggtgaggacg tcccggttgc
     7981 ggtatttgcg ccaggcgtcg aggaagaact ggcgggcctg gtcgcggctg gggtcgaaca
     8041 tgggggggct ccgggaagaa ggcggaagag ggaggaggct gaagagggag gaggcgcgtt
     8101 caggcggtct tgtcggggaa gcggcagagg tcgcgcagca ggcattcgcc gcacttgggc
     8161 gtgcgggcga cacagacgta gcggccgtgg aggatgagcc agtggtgggc gtcgcgcagg
     8221 taggccttgg gaatgcggcg caggaggcgc ttttccactt cgagcacgtc tttgccgggg
     8281 gccaggccgg tgcggttggc gaggcggaag atgtgggtgt ccacggccat ggtggggtcg
     8341 ccgaagacgg tgttgaggac cacgttggcg gtcttgcggc cgacgccggg gagggcttcg
     8401 agagcggccc ggtcgtgggg gacttcgccg ccgtggcgtt ccagcagttg ctgcgacagg
     8461 agcagggtgt tgcgggcctt gttgcggaac aggccgatgg tcttgatctg ttcggcgatg
     8521 gcgtcctcgc ccaggctcac catggcctcc ggcgtggggg cgagggcgaa gaggcggcgg
     8581 gtggcgaggt tcacgccctt gtcggtggcc tgggcggaga ggaccacggc caccaggagc
     8641 tggtaggggc tggcgtattc cagctcagtg gtggggtgag ggttggcggt ggcgaggcgg
     8701 cggaaggctt ctgcgatctc tgccaggcgc atctcggcca tgggtcagcc ggcttcgacc
     8761 ggggtgtcga tggacggcgg ggcctggcgg gcgcgggcgg ccttgcgggc ttcgagccag
     8821 ttgcggccgg cgacgaggct gcccagggcg aagaaggcac caggcgggag gatggccacc
     8881 aggaagccgg ggtagtcgtc gccgaagagg acgatgggcg aagcgccggg gatgatcatc
     8941 tcgatgcccg agagcagggt gccgctgcca acgaattcgc gcagggcgcc caggaccacc
     9001 agcacgaggg tgaggccggc gccctgggcg atgccgtcca gggtggagcc gaggggctcg
     9061 ttcttggcgg cgaaggcttc gacgcgggcg agcacgatgc agttggtgac gatgagcggg
     9121 atgaagatgc cgagcac
//
LOCUS       DAOQPA010000127         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4095_length_9121_cov_18.831458, whole genome shotgun sequence.
ACCESSION   DAOQPA010000127 DAOQPA010000000
VERSION     DAOQPA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4095_length_9121_cov_18.831458"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /gene="nirB"
                     /locus_tag="PKX01_14440"
     CDS             complement(<1..1956)
                     /gene="nirB"
                     /locus_tag="PKX01_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HMZ77378.1"
                     /translation="MRKLKLVMVGNGMAGVRTLEELRKLAPDMYDITVFGAEPHPNYN
                     RILLSPVLAGEMTIQDIILNDLQWYADNGITLHLGSKVTSIDRQNRSVVATDRDGQTI
                     TVDYDRLLLATGSNPFILPIPGNDLPGVISYRDIKDTDEMIDAAAKYKHAVVIGAGLL
                     GLEAANGLALRGMDVTVVHISDWIMERQLDRTAGKMLQKALEEKGMKFKLNAQTAELV
                     RGESGRVCAIKFKDGETLPADLVCMAAGIRPNVDLAEKAGIHCNRGIIVNDTLQTYDP
                     AIYAVGECANHRGTAYGLVAPLFEQAKVCANHLAEIGIARYEGSVTSTKLKVTGIDVF
                     SAGDFSGSEGTEDIVLHDPGLGVYKRIVLKDDKLVGAVMYGDTKDGAWYFQLLKDEQN
                     IHDIRDHLVFGNAHLGDVGHKGTNVAASMPDTAEVCGCNGVCKGTIVKAIQEHGLFTL
                     EDVRKHTKASSSCGSCTGLVEQILASTIGGAYEPADSANKPVCGCTDHSHGDVRRAIR
                     EHKLLSVGDTQKFLEWKTPNGCATCRPALNYYCISTWPHEALDDPQSRFINERAHANI
                     QKDGTYSVVPRMWGGLTTPGELRAIADAAEKYKVPTVKVTGGQRIDLLGVKKEDLPAM
                     WADFAQAGMVSGHAYGKSLRTVKTCVGA"
     gene            complement(2296..2808)
                     /locus_tag="PKX01_14445"
     CDS             complement(2296..2808)
                     /locus_tag="PKX01_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HMZ77379.1"
                     /translation="MHSTLARLPLRVLALALFAVCGALLAFGLYLQHVKGIEPCPMCI
                     MQRYALLGCGLIGLLAGLHAPGPGGTRAYAGLLMLTALAGGGVAARQSWIQWFPPAVS
                     ECGPGLEYMLESFPLSSALPMIFRGAGDCSLVDWTFLGLSIANWSFLCFAVILLAALA
                     VLTRRSTPGA"
     gene            complement(2890..3789)
                     /gene="argB"
                     /locus_tag="PKX01_14450"
     CDS             complement(2890..3789)
                     /gene="argB"
                     /locus_tag="PKX01_14450"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMZ77380.1"
                     /translation="MTQPSLTADLSPAQKAAIIAEALPYIRRFQDKTLVIKYGGNAMT
                     DPQLKACFAHDVVLLKLVAFNPVVVHGGGPQIENMLAKVGKKGEFIQGMRVTDAETME
                     LVEMVLGGQVNKEIVSLINQAGGKAVGLTGKDSNFIRAKKLMMENKDVPGDLIDIGQV
                     GDITQIDPSLISFLDKGDFIPVIAPIGVGEDGESYNINADVVAGKLAEILKAEKLVLL
                     TNTPGVLDKSGKLLTGLTPRQIDDLVADGTLSGGMLPKIGSALDAARNGVKSVHIIDG
                     RVEHCLLLEILTDHGVGTMIKSK"
     gene            complement(3899..4315)
                     /locus_tag="PKX01_14455"
     CDS             complement(3899..4315)
                     /locus_tag="PKX01_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ77381.1"
                     /translation="MSTLTRRDPLDDFFRGFFVRPVDFAGSPAEAPQMRVDVKETPEG
                     YQVHAELPGVKKEDIHIHIDGPVVSISAERKQDKEVKEGERILRTERYFGQVSRSFQL
                     GQDVDEAAANARFSDGVLELTLPKKVAPKAKRLIIE"
     gene            4510..4647
                     /locus_tag="PKX01_14460"
     CDS             4510..4647
                     /locus_tag="PKX01_14460"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77382.1"
                     /translation="MTAGDTTCWCASLPPLLAVPTAPTTAACYCPACLRRMLAAQPDQ
                     P"
     gene            complement(4610..5089)
                     /gene="nudB"
                     /locus_tag="PKX01_14465"
     CDS             complement(4610..5089)
                     /gene="nudB"
                     /locus_tag="PKX01_14465"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HMZ77383.1"
                     /translation="MSGIKQPVSVLVVIHTPALDVLLLERAGPSGLWQSVTGSREGDE
                     TLAATARREVAEETGLLLPDAANGLRDLRLANRFPILPRWRARYPEGVTHNTEHVFSL
                     CIPEPVPVRLAPDEHVAARWLPWQDAAAQVFSWTNRDAIRLVADRLRADPAAPPASA"
     gene            complement(5086..5466)
                     /locus_tag="PKX01_14470"
     CDS             complement(5086..5466)
                     /locus_tag="PKX01_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HMZ77384.1"
                     /translation="MFTPEFDSDDDETVDTPLIRIDLEGVTDKAGFLAAVAAALHFPA
                     HFGHNWDALADCLTDLSWLPRAPGYAIAFDHADAFAAAAPEAFATALSIFQEAADFWR
                     AEGVALWTLVGSTQTVTPDFQDPT"
     gene            complement(5517..5912)
                     /locus_tag="PKX01_14475"
     CDS             complement(5517..5912)
                     /locus_tag="PKX01_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HMZ77385.1"
                     /translation="MSRLLPLFRCLLVLLCLIGSSAATARSPWDDLIGSRADRATIPL
                     RALPPEARDTLARIRAGGPFPFRRDGIAFQNRERRLPAAEPGFYREYTVITPGADGRG
                     ARRIISGGWPPTIYYYTDDHYRSFRRIAE"
     gene            complement(5973..7589)
                     /locus_tag="PKX01_14480"
     CDS             complement(5973..7589)
                     /locus_tag="PKX01_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ77386.1"
                     /translation="MIDLDTITALVVEANPTMRTQLRNMLNLSGISKVQFAVTAGSAV
                     RKLRDNRFDLILCEYHLGEGQDGQHLLEDLRLHHIIPLSTLFIMVTGERQYERVVSAA
                     ELAPNDYILKPFAADTLQERIERALTKRDAFMPAYRLIELGNAPDAIAVCAAGENDHP
                     QWAVDFMRLRAELLLTCGKADDAHKLYSKILSLRSIPWARLGLAKALFLQKRFAEAES
                     LLQGLVGENDMYLDAYDWLSRTREAAGELEAACKVLATATAVSPHRVGRLRKLGELAI
                     ETGDHDTAEKVLSEVVRKGKYSDFRDPEDHVRLLQAQLAKGDTAQAESTIRDLERTMP
                     GLDKTRPCATLSSALLHLKKGNADKAGEALRALTSEGSNAHDLSVGLKRELAKACFAS
                     QLEDHGSEVVLDLMRNAADERSMERAKAVLRDAGKAELGETLAAQMQMEVRDLVSEGA
                     RKAQEGDFDGAVQLMMNAVRKMRGNVHVLFNATLALLKHVENCGWNEQFAHQARDLME
                     RARGQDPGNARLPALTAYYYTLLKKYGIQP"
     gene            complement(7719..>9121)
                     /gene="aspS"
                     /locus_tag="PKX01_14485"
     CDS             complement(7719..>9121)
                     /gene="aspS"
                     /locus_tag="PKX01_14485"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMZ77387.1"
                     /translation="QKNMILRYRTAMAFRRFLDAQGFIDIETPMLTKSTPEGARDYLV
                     PSRVHDGQFFALPQSPQLFKQMLMVAGYDRYYQITKCFRDEDLRADRQPEFTQVDIET
                     SFLTEADITAMMEEMIRTVFQEVLSVELPAPFPRMTYAEAMDRFGSDKPDLRVTLELT
                     EVTDLVKDVAFKVFSGPANSGGRVAAMRVPGGAAGDRVLSRGEIDEYTKFVGIYGAKG
                     LAYIKVNDASQPNESGLQSPIVKNLHETALRAILERTGAQSGDLIFFGADKTKVVNDA
                     LGALRIKLGHEKGYVNGKAWEPLWVVDFPMFEYDEEDKRWTACHHPFTSPKDEHLELL
                     TSDPGKCLAKAYDLALNGWEIGGGSVRIHRADVQEKVFTALNIGPEEQQLKFGFLLDA
                     LKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRAQCLLTNAPSPVDEKQLRE
                     LHIRLRNIKAPDAPAA"
BASE COUNT         1391 a         2686 c         3383 g         1661 t
ORIGIN      
        1 ggcgccgacg caggtcttca cggtgcgcag gctcttgccg taggcgtggc cggagaccat
       61 gccggcctgg gcgaagtcgg cccacatggc gggcaagtct tccttcttca cgcccagcag
      121 gtcgatgcgc tggccgccgg tgaccttgac ggtgggcacc ttgtactttt cggcggcgtc
      181 ggcgatggcg cgcagctcgc ccggagtggt cagcccgccc cacatgcgcg gcacgacgga
      241 gtaggtgccg tccttctgga tgttggcgtg ggcacgctcg ttgatgaagc ggctctgggg
      301 atcgtccagg gcttcatggg gccacgtgga gatgcagtag tagttcaggg ccgggcggca
      361 ggtggcgcag ccgttgggcg tcttccattc gaggaatttc tgcgtgtcgc cgacggacag
      421 cagcttgtgc tcgcggatcg ccctgcgcac gtcaccatgg gaatggtcgg tgcagccgca
      481 taccggcttg ttggcggagt cggccggctc gtaggcgccg ccgatggtgg aggcgaggat
      541 ctgttccacc agaccggtac aggagccgca ggagctggag gccttggtgt gcttgcgcac
      601 gtcttccagc gtgaagaggc cgtgctcctg gatggccttg acgatggtgc ccttgcatac
      661 gccgttgcag ccgcacactt cggcggtgtc gggcatgctg gcggccacgt tggtgccctt
      721 gtggcccacg tcgcccaggt gggcgttgcc gaagaccagg tggtcgcgga tgtcgtggat
      781 gttctgctcg tccttgagga gctggaagta ccaggcacca tccttggtgt cgccgtacat
      841 cacggcgccc accagcttgt cgtccttgag gacgatgcgc ttgtacacgc ccaggcccgg
      901 atcgtggagg acgatgtcct cggtgccttc gctgccggaa aagtcgccgg cggagaacac
      961 gtcgatgccg gtcaccttga gcttggtgct ggtgacggag ccttcgtaac gggcgatgcc
     1021 gatctcggcc agatggttgg cgcacacctt ggcctgttcg aacaggggcg cgacgaggcc
     1081 gtaggcagtg ccgcggtggt tggcgcattc gcccaccgcg tagatggccg ggtcgtaggt
     1141 ctgcagggtg tcgttgacga tgatgccgcg gttgcagtgg atgccggcct tttccgccag
     1201 atccacgttg gggcggatgc cggcggccat gcacaccagg tcggccggga gggtttcgcc
     1261 gtccttgaac ttgatggcgc acacccggcc ggattccccg cgtaccagtt ctgcggtctg
     1321 ggcgttgagc ttgaacttca tgcccttttc ttcgagggcc ttctgcagca tcttgccggc
     1381 ggtgcggtca agctggcgtt ccatgatcca gtcggagatg tgcaccacgg tcacgtccat
     1441 gccgcgcagg gccaggccgt tggccgcttc caggccgagc aggccggcgc cgatcaccac
     1501 cgcgtgcttg tatttggccg cagcgtcgat catctcgtcg gtgtccttga tgtcccgata
     1561 cgagatgacg cccggcaggt cgttgccggg gatggggagg atgaagggat tggagccggt
     1621 ggccagcagc agccggtcgt aatcgacggt gatggtctgg ccgtcgcggt cggtggcgac
     1681 caccgagcgg ttctggcggt cgatcgacgt gaccttgctg cccaggtgca gggtgatgcc
     1741 gttgtcggca taccactgca ggtcattcag gatgatgtct tgaatggtca tctcgccggc
     1801 gagcaccggg gagagcagga tacggttgta gttggggtgg ggctcggctc cgaagaccgt
     1861 gatgtcgtac atgtccgggg ccagcttccg cagttcttcc agggtgcgga cgcccgccat
     1921 accgttgccg accatgacca gtttgagttt gcgcatgatt cctccgacga ttgaatctcc
     1981 ccggaccggt tcgcgcaggg cggccgatgc gggcgtaaag cctgtgggca ggggagatcg
     2041 tcgtcgatcg tggcgggacc tccgttggcc ttgccacgtc gccccgggca gtggggcgcg
     2101 caaacacctg gatcaggggg ctgcactgcg gcaatgccgt tattgccgtg cgcagcttgc
     2161 gttggagaaa taaaagcaac ggtcatgcca gggcgcgccc cccgcgtggg ccgcgggatt
     2221 cgggcatttc aggcggcaga cgcgcgctgc ggatcgcact gcactggtgc gcactgctcc
     2281 gccgtggggc gcgtttcagg cgccgggtgt gctgcgacgc gtcaggacgg caagggcggc
     2341 caggaggatg accgcgaagc acaggaagga ccaattggcg atggacaggc ccaggaaggt
     2401 ccagtccacc agcgagcagt caccggcgcc gcgaaagatc atgggcagcg ccgacgacag
     2461 cgggaagctc tccagcatgt attccagacc cggaccacat tcggagaccg ccggcggaaa
     2521 ccactggatc catgactggc gggccgccac cccgccgccg gccagtgccg tgagcatgag
     2581 gaggcctgcg taggcacgtg tgccgccggg gcccggcgcg tgcaggcccg ccaacagccc
     2641 gatgaggccg cagccgagca gggcatagcg ctgcatgatg cacatgggac agggctcgat
     2701 gcccttgacg tgctggaggt acaagccgaa ggcgagcagg gcgccgcaga ctgcgaacag
     2761 ggcgagggcc aggacgcgga ggggcaggcg ggcgagcgtg gaatgcatgg gagattcggc
     2821 agggggcgcc ggcgatgggg gcctgcggtg gaccggggaa ggcagggcgc gttccccggc
     2881 cctcaggcct tatttgctct tgatcatcgt gccgacgccg tggtcggtga ggatttcgag
     2941 gagcaggcag tgttcgacgc ggccgtcgat gatgtgcacg ctcttcacgc cgttacgggc
     3001 ggcgtcgagg gcagaaccga tcttgggcag catgccgccg gacagggtgc cgtcggccac
     3061 cagatcgtcg atctggcgcg gggtgaggcc ggtgagcagc ttgccggact tgtccagcac
     3121 gcccggggtg ttggtgagca gcaccagctt ctcggccttg aggatctcgg ccagcttgcc
     3181 ggccaccacg tcggcgttga tgttgtagct ctcgccgtcc tcgccgacgc cgatgggggc
     3241 gatcaccggg atgaagtcgc ccttgtcgag gaaggagatc aggcttgggt cgatctgggt
     3301 gatgtcaccc acctggccga tgtcgatcag gtcgcccggc acgtccttgt tctccatcat
     3361 cagcttcttg gcgcggatga agttggagtc cttgccggtg aggcccacgg ccttgccgcc
     3421 agcctggttg atgaggctga cgatctcctt gttcacctgg ccgcccagca ccatctcgac
     3481 cagttccatg gtctcggcgt cggtgacgcg catgccctgg atgaattcgc ccttcttgcc
     3541 caccttggcg agcatgttct cgatctgcgg tcccccgcca tggacgacca ccgggttgaa
     3601 ggccaccagc ttgaggagca ccacgtcgtg ggcaaagcag gccttgagct gcgggtcggt
     3661 catcgcattg ccgccgtact tgatgaccag ggttttgtcc tggaagcggc ggatgtaagg
     3721 cagggcctcg gcgatgatgg cggccttctg ggccgggctg aggtcggcgg tgagggaggg
     3781 ctgggtcatg gtgcgagcgc ggtagtgggc gggtaggcgc gattgtaatg acggggacgg
     3841 caaacaaaaa gaaaaagcgg gaagatcgct cttcccgccg gggatgtggc gtgggggctt
     3901 attcgatgat cagccgtttg gccttgggcg ccaccttctt gggcagcgtc agttcgagga
     3961 caccgtcgct aaagcgggca ttggccgcag cttcatccac gtcctgaccg agctggaagc
     4021 tgcgcgaaac ctggccgaag tagcgttcgg tgcgcaggat gcgttcccct tccttcacct
     4081 ccttgtcctg cttgcgctcg gcgctgatgg acaccaccgg gccgtcgatg tggatgtgga
     4141 tgtcttcctt cttcacgccc ggcagttcgg catgcacctg atagccttcc ggggtttcct
     4201 tcacgtccac acgcatctgg ggcgcttcgg cggggctgcc ggcgaagtcc acggggcgga
     4261 cgaaaaagcc gcggaaaaag tcgtccagag ggtcgcggcg agtgagggtg ctcatgcggg
     4321 ttctcctttc gtcagtgagc gtcgcgggcg atctgcccgc cttgtatcca atatggggat
     4381 cgggatgctg gcttcaaggg ccggcaagcc cctagactgc cggctggacg tttcggattg
     4441 agcacgatgg agcagacgaa ggaacccccc aacctgtgcc ccgcctgtgg cacgcgcttc
     4501 acctgcggga tgacggccgg cgacacgacg tgctggtgtg catccctgcc gcccctgctg
     4561 gcggtgccga cggccccgac gacggccgcc tgctactgcc cggcctgcct taggcggatg
     4621 ctggcggcgc agccggatca gccctgaggc gatcggccac gaggcggatg gcgtcccggt
     4681 tggtccacga gaacacctgc gcggcggcgt cctgccaggg cagccaccgg gcggccacgt
     4741 gctcgtcggg ggcgagtcgc accggcacgg gttccggaat gcacaggctg aagacgtgct
     4801 cggtgttgtg ggtgacgccc tccggataac gggcacgcca gcggggcagg atggggaagc
     4861 ggttggcgag gcgcaggtcg cgcaggccgt tcgcagcgtc ggggagcagg agaccggtct
     4921 cctccgccac ttcccggcgg gcagtggccg ccagggtttc gtccccctcg cggcttccgg
     4981 tgaccgattg ccacaggccg gacgggccgg cgcgttcgag gagcaggacg tccagggccg
     5041 gggtatggat caccaccagc accgagacgg gctgcttgat gccgctcatg tcgggtcctg
     5101 gaagtccggc gtgacggtct gggtggagcc caccagggtc cacagggcga cgccttccgc
     5161 ccgccagaaa tcggccgcct cctggaagat ggacagggcg gtggcgaagg cctccggcgc
     5221 cgcagcggcg aaggcgtcgg cgtggtcgaa ggcgatggcg tagccggggg ctcgcggcag
     5281 ccaggacagg tcggtgaggc aatccgccag ggcgtcccag ttgtggccga agtgggcggg
     5341 aaaatgcagg gctgcggcca cggcggcaag aaagccggcc ttgtcggtga cgccctccag
     5401 gtcgatgcgg atcagggggg tgtcgaccgt ctcgtcgtcg tcggaatcga attcaggggt
     5461 gaacatggga ggtgtccagg tgccgggtga gcgggcgcgg gctgtccgcg ggccggtcat
     5521 tcggcgatgc ggcggaagct gcggtagtgg tcatcggtgt agtagtagat cgtgggcggc
     5581 catccgcccg agatgatgcg acgggcgcca cggccgtcgg cgccgggggt gatcaccgta
     5641 tattcgcggt agaagccggg ttcggcggcg ggcaggcggc gctcccgatt ctggaaggcg
     5701 ataccgtcgc ggcggaaggg aaacggtccg ccggcccgga tacgtgcgag ggtgtcgcgg
     5761 gcttccggcg gcagggcacg aagcgggatc gttgcgcggt ccgcgcgcga tccgatcagg
     5821 tcgtcccagg gtgaccgtgc ggtggccgcg gatgagccga tcaggcacag caggacaagc
     5881 aggcaccgga acaggggcaa cagtcgggac atcggggacg gacgggaatg ggggtggcgg
     5941 cagtttagca ggccgcaggc gctgaccgcg cctcagggct ggatgccgta tttcttgagc
     6001 agggtgtagt aataggccgt cagggcgggc agccgcgcgt tgcctggatc ctgaccgcgg
     6061 gcacgttcca tgagatcccg cgcctgatgg gcgaactgct cattccagcc gcagttttcc
     6121 acgtgcttga ggagggccag ggtggcgttg aacaggacgt ggacgttgcc ccgcatcttg
     6181 cgtacggcgt tcatcatgag ctgcacggcg ccatcgaagt cgccttcctg ggctttgcgg
     6241 gcaccttcgc tgacgaggtc gcgaacctcc atctgcatct gcgcagcgag ggtttcaccc
     6301 agttcggcct tgcccgcgtc ccgcagcacc gccttggcgc gctccatact gcgttcgtcg
     6361 gcggcattgc gcatgagatc gaggacgacc tcgctgccat ggtcctcgag ctgactcgca
     6421 aagcaggcct tggcgagttc ccgcttgagg ccgacggaca ggtcgtgggc attgctgccc
     6481 tccgacgtga gggcacgcag ggcttcgccg gccttgtcgg cgttgccttt cttgaggtgc
     6541 aggagggcgc tcgaaagcgt ggcgcagggt cgggtcttgt ccaggccggg catggtgcgc
     6601 tccaggtccc ggatggtgga ctcggcctgg gccgtgtcgc ccttggcgag ctgggcctgg
     6661 agcaggcgga cgtggtcttc cgggtcccgg aagtcggaat acttgccctt gcgcaccact
     6721 tcggacagca ccttctcggc ggtgtcgtgg tcgccggtct cgatggccag ctcgccgagc
     6781 ttgcgcagtc ggccgacccg gtggggcgac accgcggtgg cggtggcgag caccttgcac
     6841 gcggcttcca gttcgccggc ggcttcgcgg gtgcgggaca gccagtcgta ggcgtccagg
     6901 tacatgtcgt tttcgccgac caggccctgc aacaggcttt cggcttcagc gaaccgcttc
     6961 tgcaggaaga gggccttggc gaggcccagg cgggcccacg gaatgctgcg caggctgagg
     7021 atcttcgagt acagcttgtg ggcgtcgtcg gccttgccgc aggtcagaag cagttcggcg
     7081 cgcaggcgca tgaagtccac cgcccactgg ggatgatcgt tttcgcccgc ggcgcagacg
     7141 gcaatggcat ccggggcgtt gcccagttcg atcaggcgat aggccggcat gaaggcgtcg
     7201 cgcttggtga gggcccgctc gatgcgctcc tgcagggtgt cggcggcgaa gggcttgagg
     7261 atgtagtcgt tgggggcgag ctcggcggcg ctgacaaccc gttcgtactg gcgttcgccg
     7321 gtgaccatga tgaacagggt cgacaggggg atgatgtggt ggaggcgcag gtcttcgagc
     7381 aggtgttggc cgtcctgccc ttcgcccagg tggtattcgc agaggatcag gtcgaagcgg
     7441 ttgtcccgca gcttgcgtac tgcggagccc gcggtgacgg cgaactggac cttgctgatg
     7501 ccgctcaggt tgagcatgtt gcgaagctgc gtccgcatgg tgggattggc ttcgacgaca
     7561 agggcagtga tggtgtcgag atcgatcacg ggggcagccg ggcagacgcg ggaacgatga
     7621 atgcatcggc ccggtccggg gtttcttgag tgcgacgtcc acgaaaaagg gcgaacggga
     7681 tgtcccgttc gccctggcgg agacggtctt gccgatgctt acgcggcggg tgcgtccggc
     7741 gccttgatgt tgcgcaggcg gatgtgcagt tcgcgcagct gcttctcgtc cacaggagac
     7801 ggggcgttgg tgagcaggca ctgggcgcgc tgggtcttgg ggaaggcgat cacgtcgcgg
     7861 atggactcgg cgccggtcat catggtgacg atgcggtcaa ggccgaaggc gaggccgccg
     7921 tgggggggcg cgccgtactt gagggcgtcc agcaggaagc cgaacttgag ctgctgctcc
     7981 tccggcccga tgttgagggc ggtgaagacc ttttcctgca cgtcggcccg gtgaatacgc
     8041 acggagccgc ccccgatctc ccagccgttg agggccaggt cgtaggcctt ggcgaggcac
     8101 ttgcccggat cgctggtgag cagttcgagg tgctcgtcct tggggctggt gaaggggtgg
     8161 tggcaggcgg tccagcgctt gtcttcctcg tcgtactcga acatggggaa gtccacgacc
     8221 cacaggggtt cccaggcctt gccgttgacg tagccctttt cgtggccgag cttgatgcgc
     8281 agggcgccga gggcgtcatt gacgaccttg gtcttgtcgg cgccgaagaa gatcaggtcg
     8341 ccggactggg cgccggtgcg ttccaggatg gcgcgcaggg cggtttcgtg aaggttcttg
     8401 acgatgggcg actggaggcc gctctcattg ggctggctgg cgtcgttgac cttgatgtag
     8461 gcgaggccct tggcgccata gatgccgaca aacttggtgt attcgtcgat ctcgccgcgg
     8521 ctcagcacgc ggtcgcccgc ggcgcctccc ggcacgcgca tggcggccac gcgaccgccg
     8581 gagttggccg ggccggagaa gaccttgaag gccacgtcct tgaccaggtc ggtgacctcg
     8641 gtgagttcca gcgtgacgcg cagatcgggc ttgtcggagc cgaagcggtc catggcttcg
     8701 gcgtaggtca tgcgcgggaa aggcgcaggc agttcgacgg acagcacttc ctggaagacg
     8761 gtgcggatca tctcttccat catcgccgtg atgtcggcct cggtgaggaa ggaggtctcg
     8821 atatcgacct gggtgaattc gggctggcgg tcggcacgca ggtcttcgtc ccggaagcac
     8881 ttggtgatct ggtagtagcg gtcgtagccg gcgaccatca gcatctgctt gaagagctgc
     8941 ggggactggg gcagggcgaa gaactggcca tcgtgcacgc gggacgggac caggtagtcg
     9001 cgggcgcctt cgggggtgct cttggtgagc atgggcgtct cgatgtcgat gaagccctgg
     9061 gcgtccagga agcggcggaa ggccatggcc gtgcggtagc gcaggatcat gttcttctgc
     9121 a
//
LOCUS       DAOQPA010000128         9059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4136_length_9059_cov_18.079187, whole genome shotgun sequence.
ACCESSION   DAOQPA010000128 DAOQPA010000000
VERSION     DAOQPA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4136_length_9059_cov_18.079187"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKX01_14490"
     CDS             complement(<1..205)
                     /locus_tag="PKX01_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HMZ77388.1"
                     /translation="MIGRLTGRLAEKAPPQILLDVNGVGYELEVPMSTFYGLPATGQT
                     VTLATHLAVREDGHFLYGFASEDE"
     gene            complement(218..829)
                     /locus_tag="PKX01_14495"
     CDS             complement(218..829)
                     /locus_tag="PKX01_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HMZ77389.1"
                     /translation="MKLVGMLDSPFVRRAFISGRMLGIPFEHQSLSVLRQVEEFRALN
                     PLVKAPTLVFDDGEVMVDSSQIITLFESLAAPGSSLLPAAPADRRRCLQLTGFGLAAA
                     EKALHRVLETRIRPKEARFPEWLARTERQLRDTWAMIDARVGDADWLCGDAPTQADIT
                     VAVAWRFTHYSAPGFLADGDYPHVAALAADAETLPAFVAADFT"
     gene            complement(842..1156)
                     /locus_tag="PKX01_14500"
     CDS             complement(842..1156)
                     /locus_tag="PKX01_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77390.1"
                     /translation="MRRVRAALLAFGLPACAAAQAPGSTAFCLFPLPAEAGAQRFINL
                     GIVQYVEVRADEVRLAYGGGNLGSGHDARIPIRSREEGEAVLDRLRRTASACRFPSPT
                     PP"
     gene            complement(1153..1836)
                     /locus_tag="PKX01_14505"
     CDS             complement(1153..1836)
                     /locus_tag="PKX01_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMZ77391.1"
                     /translation="MSPSLPFLPGDIDGVIFDMDGLLLDSERVALTIIEATAAELGLP
                     WRHEVGLAMVGLNSRDSPAVVRSHLGADFPVEALMDACGVHYERAIAAGRIPFKPGVE
                     ALFDVLDTLGLGRVVATSTRRVRAEPKLAAVGLLPRLHGMVCGDEVSRGKPAPDIFLA
                     AAARLGLPPARCLVLEDSNAGVRGALAAGARVVMVPDLLQPADDVRAAAVPVAASLAD
                     LADFFGRAP"
     gene            complement(1833..2807)
                     /locus_tag="PKX01_14510"
     CDS             complement(1833..2807)
                     /locus_tag="PKX01_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HMZ77392.1"
                     /translation="MASAPPASPAEPRVVLRTRPVLAAFLATRPAFLGVTLCACLVGL
                     ATAHGSGVIVDSTRAALTIAFALFAHAGINVLNDYYDARSGADAANTERVFPFTGGSR
                     FIQNGVLSEAETARLGWGLLLPVVPAGLWLAAQSGIGLVALGVAGLFLGWAYTAPPLA
                     LVSRGLGEIAVAAGWLIVVAGSDFVQRGAWSFLPVAAGLSYALLVAAILFFNEFPDHR
                     GDAAAGKRTLVVRFGPDGAKWSCVGLVVGAYAWLMLMVGRELLPHAAAAAAFSLILSF
                     RALRHLLAHAAEPAALAPALKLTIAAAHLHGLILAATLAFARWPGGGA"
     gene            3226..4395
                     /locus_tag="PKX01_14515"
     CDS             3226..4395
                     /locus_tag="PKX01_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77393.1"
                     /translation="MPCRSAPPLLYPSLGALLWVAAHSAAAATLTVSPDGPIRRIADA
                     ARLAKDGDTVLIRAGTYRHDVASWPQRSLTIRGVGGRPILDAAGEGAEGKAIWVLKDG
                     HFRIDNIAFRGARVPDGNGAGIRLEHGRLDLSRCVFEDNQMGLLTSDDATVELNIRDS
                     LFRRAPPQRKPLPHLLYAGQIRLLTVQGSRFEQGYEGHLVKSRARRSELKYNLLADGP
                     GGQASYELDLPNGGVAVVVGNVIAQSAATRNPALVSYGAEGALWPENRLLLAHNTLIT
                     TGPQAAWFARAWRERLPADTQVVTRNNLTVGPGVFNLGLGGDHRGDTALTGPAPTPPD
                     YALPAASPLRGRVTAAEQDGEGLPAAEFAWPVGTRPLAAPARWAPGAMQRHASGN"
     gene            complement(4409..5014)
                     /locus_tag="PKX01_14520"
     CDS             complement(4409..5014)
                     /locus_tag="PKX01_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HMZ77394.1"
                     /translation="MVRKTKEEAETTRRQIIDAARRVFHECGVSRTSLEKIATAAGVT
                     RGAVYWHFKNKTELFFAMRDEAKLPMVDRVVFDPCDENPLGGIETALREMVQVLIDQP
                     ETRETFEIVSFKCEYVDEFALALQGNNCQMDFLDKLAAAYARAAEKGQLRAGFAPEAL
                     AHDTFLFVGGLIKHWLARMPDERFRQRAGDMIRAHVALRRA"
     gene            5161..6357
                     /locus_tag="PKX01_14525"
     CDS             5161..6357
                     /locus_tag="PKX01_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77395.1"
                     /translation="MTARPNALLTSVPVLLAATLLAACGSKDDKAGGMPPGGMPALPV
                     TVLEMQPTKVETSVEAVAQTEGPREVEVRARVGGILVKRLYDEGAAVKAGQPLFQIDR
                     EPYEVAVAQAKAQVAEAKARVDQTRREAARLKGLLAQQAISQREYDTATSDDAAAAAA
                     VLAAEAALRQAQLNLEYATVTAPVAGLTGRVAVSEGNLLATGGGTLLTTVVQVNPMWV
                     RFSLAEADLAAATPGGRFNPKAVRGVELVLPDGSTYPVRGHLNFSASQVDPKLGTLQL
                     RGEFANPDNVLLPGQFVRARLLTGERDGVFKVPQNAVMQTDMGAVVMLANADNKVEPR
                     PVKTGNWSGKDWVILGGLKAGDKVIVDNLIKIRPGAPVAPHGPGEGPGAPGGQPGAPA
                     AAPAKG"
     gene            6371..>9059
                     /locus_tag="PKX01_14530"
     CDS             6371..>9059
                     /locus_tag="PKX01_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ77396.1"
                     /translation="MSRFFISRPIFASVISIIIVIAGLMASLTLPVAQYPEITPPTII
                     ISASYPGANAETLSKTVAAPIEEQLSGAEGLIYYNSQSTSNGTVTITATFEVGTNPDN
                     ALIAVNNRVKVAEPRLPDDVRRTGVLVQKRSNNILMFAAVRSPKGAFDTLYLSNYVAL
                     NIIEEIRRIPGVGDAQQFDAIYAMRVWLKPDVMAKLGITTTDIANAIRVQNTQNAAGK
                     IGQEPVVDGQQLTYTVTAKGRLLTPEEFGGIILKAEGPNGIVRIRDVARIELGAENYD
                     RSTAVNGAPVSGMGIYLQSGANALDTAKRVRARLDEMQKRFPQGMEYFIPYDTTRFIQ
                     ESAHEVVKTLVEAALLVIAVVYLFLQSWRATLIPMVAVPVSLIGTFAGMWLFGFSINT
                     LTLFAMVLAIGIVVDDAIVVLENVERLMSEQKLAPFDAAVEAMREVSGAVVAIVLVLC
                     AVFVPVAFLGGIAGKLYQQFAVTVAVAVVISGIVALTLTPALCALLLKASHEEKPLFK
                     PFNRLFERFTRSYTNTVGLTLRHGIIGTLVFLGTIGATAFLLRIVPGSFVPAEDQGYL
                     FGFVTLTDGASAQRTFAASEQMRTRIMSDDIENIFFVNGIDFITGNNRASVATTFIIF
                     KPWEQRSATADDMAKKFMGVGMSLSDGLGLVFNPPPIQGLGTAGGFEVYLQNRADGDA
                     RRLAQVSGQFLEALRKRPELTGLNSFFRVTAPQLYVEVDEPKALSMGISLDSIYATLQ
                     ATTGTLYVNDFNRAGKTYKVELQAEAAYRMNPEDLLKPYVRTASGAMVPLSAVAKVKT
                     LTGAEMVERFNGFVSAKVLGNAAPGFSSGQAIQAVEEVAREALPEGFQVEWVGQAFQE
                     KRTGSASIVAFTFGIIMVFLILAAQFEKWSLPLAVIMAVP"
BASE COUNT         1456 a         3183 c         3192 g         1228 t
ORIGIN      
        1 gctcgtcctc gctggcaaag ccgtagagga agtggccgtc ctcgcgcacc gccaggtggg
       61 tggcgagggt gacggtctgg ccggtggcgg gcaggccgta gaaggtgctc atgggcactt
      121 ccagctcgta gccgacgccg ttcacgtcga ggaggatctg gggcggggct ttttcggcga
      181 ggcgtccggt gaggcgtccg atcatggggc gttcaggtca ggtgaagtcg gcggccacga
      241 aggccggcag ggtttcggca tcggcggcga gggccgccac gtgggggtag tcgccgtcgg
      301 cgaggaagcc gggggcgctg taatgggtga agcgccaggc cacggcgacg gtgatgtcgg
      361 cctgggtggg ggcgtcgccg cacagccagt cggcgtcgcc gacgcgggcg tcgatcatgg
      421 cccaggtgtc gcggagctgg cgctcggtgc gcgccagcca ttccggaaaa cgggcctcct
      481 tgggccggat gcgggtctcg agcacgcgat ggagggcctt ttcggcggcg gcgaggccaa
      541 agccggtgag ctgcaggcag cgccgacggt cggcgggcgc ggcaggcagc aggctgctgc
      601 cgggcgcggc gagggattcg aagagggtga tgatctggct gctgtccacc atcacctcgc
      661 cgtcgtcgaa gaccagggtg ggcgccttga ccagcgggtt gagggcgcgg aattcctcga
      721 cctggcgcag caccgacagg gactggtgct cgaaggggat gcccagcatg cggccactga
      781 tgaaggcgcg acggacaaag ggggaatcga gcatgccgac gagtttcatg gagacctccg
      841 gtcagggcgg ggtgggggaa gggaaacgac aggcggacgc ggtgcgacgc aggcggtcga
      901 ggacggcctc gccttcttcg cgggaacgga tcgggatgcg cgcgtcgtgg ccgctgccca
      961 ggttgccgcc gccgtaggcg aggcgcactt cgtcggcgcg gacctccacg tactgcacga
     1021 tgcccaggtt gatgaagcgc tgggcgccgg cctcggccgg tagcgggaac aggcagaagg
     1081 cggtggagcc gggtgcctgg gcggccgcgc aggcgggcag cccgaatgcc agcagggcgg
     1141 cgcggacgcg tctcatgggg cgcgcccgaa gaagtcggcc agatcggcga ggctcgcggc
     1201 caccggcacg gcggcggcgc gcacgtcgtc ggcgggctgg agcaggtccg gcaccatcac
     1261 cacccgcgcc ccggcggcga gggcgccgcg cacgccggcg ttggaatcct ccagcaccag
     1321 gcagcgggcc ggcggcaggc cgagccgcgc ggcggcggcc aggaagatgt ccggcgcggg
     1381 cttgccgcgg ctcacctcgt cgccgcacac catgccgtgc aggcgcggca gcaggcccac
     1441 cgcagcgagc ttgggctcgg cccgcacgcg gcgcgtggag gtggccacca cgcggcccag
     1501 gccgagggtg tcgaggacgt cgaagagggc ctccacgccg ggcttgaagg ggatgcgccc
     1561 tgcggcgatg gcccgttcgt agtgcacgcc gcaggcatcc atcagggcct ccacggggaa
     1621 gtctgcgccc aggtggctgc ggacaacggc gggggaatcc cgcgagttga gccccaccat
     1681 ggcgagcccg acctcgtgac gccagggcag gcccagttcg gccgcagtgg cttcgatgat
     1741 ggtcagggcg acccgctcac tgtccagcag caggccgtcc atgtcgaaga tgacgccgtc
     1801 gatgtcgccc ggcaggaagg gcagggacgg gctcatgcgc cgcctcccgg ccagcgcgcg
     1861 aaggccagcg tggcggcgag gatcagcccg tgcaggtgag ccgcggcgat ggtgagcttg
     1921 agggcaggcg ccagggcggc cggctcggca gcgtgggcga gcaggtggcg cagggcacgg
     1981 aaggacagga tcaggctgaa agccgcggcc gccgccgcgt ggggcagcag ctcgcgcccc
     2041 accatcagca tcagccaggc gtaggcgccc accacgaggc cgacgcagga ccacttggcg
     2101 ccatccggcc cgaagcggac caccagggtg cgcttgccag cggcggcatc gccgcggtgg
     2161 tccgggaatt cgttgaagaa caggatcgcc gccaccagca gcgcgtagga cagccctgcc
     2221 gccaccggca ggaacgacca ggcgccgcgc tgcacgaagt cgctgcccgc caccacgatg
     2281 agccagcccg ccgccacggc gatttctccc aggccgcggc tcaccagggc aaggggcggc
     2341 gcggtgtagg cccagcccag gaacaggccg gcgacaccca gggcgaccag gccgatgccg
     2401 gactgggccg ccagccacag cccggcaggc accaccggca gcagcaggcc ccagcccagg
     2461 cgcgcggtct cggcctcgga gagcacgccg ttctggatga agcggctgcc gccggtaaag
     2521 ggaaagaccc gctccgtgtt ggccgcgtcg gcaccgctgc gggcatcgta atagtcgttg
     2581 aggacgttga tgccggcgtg ggcgaagagg gcgaaggcga tggtcagcgc cgcgcgggtg
     2641 gaatccacga tcacgccgct gccgtgggca gtggccagcc ccaccaggca ggcgcacagg
     2701 gtgacgccga gaaaggccgg ccgggtggcg agaaaggccg ccaggacggg gcgggtgcgc
     2761 aggacgacgc ggggttcggc aggagacgcg ggaggagcgg acgccatcgg gacagcatga
     2821 aggtgggaag cccccatcct gcgacgggcc ggggcgccgg tcaagggcgc ggcgccggac
     2881 ggacagatgg cgggccgggc cgtgcacacg ccacggggac gcggccgggc gggaaccgac
     2941 aaggcacgcg catcccgccg gcggggtgct ccggccggcg acggcggaca gtcaatggcg
     3001 gggtcccgcg ccgccgcgcc cgatgcccga gcgccgcggg cttgcgcagc cggccgccac
     3061 gcaggctcac gtttgtgcgc accagggcgc aggggcgcag gcgtaccatc gcgatcgaca
     3121 cccaccgtga ccgggacgcc tgttccgccc ggtggcccct tccaccggcc gaccccgtgg
     3181 gacgcgacgg cgtcccccaa tcgcccattt cccttttccg acccgatgcc ttgtcgatcc
     3241 gccccgcccc tgctgtatcc aagcctcggt gccctgctgt gggtcgccgc ccacagtgcc
     3301 gccgcggcca ccctcacggt gagcccggac gggccgatcc gccgcatcgc cgacgcggca
     3361 cgcctggcca aagacggtga cacggtgctg atccgcgccg gcacctaccg ccacgacgtg
     3421 gccagctggc cccagcggag cctgacgatc cgcggcgtgg gcggccggcc catcctggac
     3481 gcggcgggcg agggcgccga aggcaaggcg atctgggtgc tcaaggacgg gcatttccgc
     3541 atcgacaaca tcgcattccg cggcgcccgc gtgccggacg gcaacggcgc cggcatccgc
     3601 ctggaacacg gccgcctgga cctgagccgc tgcgtcttcg aggacaacca gatggggctg
     3661 ctcacctcgg acgacgccac ggtggaactg aacatccgcg acagcctgtt ccgccgcgcg
     3721 cccccccagc gcaagccgct gccgcacctg ctctacgccg gccagatccg cctgctgacg
     3781 gtgcagggca gccgcttcga gcagggctac gagggccacc tggtgaagag ccgcgcccgc
     3841 cgcagcgagc tgaagtacaa cctgctcgcc gacggccccg gcggtcaggc ctcgtacgaa
     3901 ctggatctgc ccaatggcgg cgtggcggtg gtggtgggca acgtgatcgc ccagagcgcc
     3961 gcgacgcgca acccggccct cgtctcttac ggcgccgaag gcgcgctgtg gccggagaac
     4021 cgcctgctgc tggcccacaa cacactgatc accaccggcc cgcaggcggc gtggttcgcc
     4081 cgcgcctggc gcgaacgcct gccggcggac acccaggtcg tgacccgcaa caacctgacg
     4141 gtcggtccgg gcgtcttcaa tctgggcctg gggggcgatc atcgcggtga cacggcgctg
     4201 accgggccgg cgccgacacc gccggactac gccctgcccg ccgcttcgcc cctgcgcgga
     4261 cgggttacgg cggcggagca ggacggcgag ggtctgccgg cagcggaatt cgcatggccc
     4321 gtgggcaccc ggccgctggc cgcaccggcc cgctgggcac cgggggccat gcagcgccac
     4381 gcctccggca actgagccgg caccgcgctc aggcccggcg cagggccacg tgggcgcgga
     4441 tcatgtcgcc ggcccgctgg cggaaacgct cgtcgggcat gcgcgcgagc cagtgcttga
     4501 tgagcccgcc gacgaacagg aaggtatcgt gagccagggc ttccggcgca aaaccggcgc
     4561 gcaactggcc cttctcggca gcgcgggcgt aggcggcggc gagtttgtcg aggaaatcca
     4621 tctggcagtt gttgccctgc agggcgaggg cgaactcgtc cacgtactcg cacttgaagc
     4681 tgacgatctc gaaggtctca cgggtttccg gctggtcgat gagcacctgc accatctccc
     4741 gcagggcggt ctcgatgccg ccgaggggat tctcgtcgca gggatcgaag accacccggt
     4801 ccaccatggg cagcttggcc tcgtcgcgca tggcgaagaa cagctcggtc ttgttcttga
     4861 aatgccagta cacggcgccc cgcgtcaccc cagcggcggt ggcgatcttc tccagcgagg
     4921 tgcggctcac cccgcactcg tgaaacaccc ggcgcgcagc atcgatgatc tggcggcggg
     4981 tggtttcggc ttcttccttg gttttgcgga ccatatggga tactccgaca gcatgggtcg
     5041 ggcagcaggc tgcatccact gcagacgcag cgacaaatgt agcgaaaggt atttacatac
     5101 attcgtgttt gtataaacta tgacgcaaac tgtcccaaaa atccagtccc ggaggtcccc
     5161 atgaccgcgc gccccaacgc cctgctcacg tccgtgcccg tcctgcttgc cgccacgctc
     5221 ctcgccgcct gcggcagcaa ggacgacaag gccggcggga tgccccccgg cggcatgccc
     5281 gccctgcccg tcaccgtcct cgagatgcag cccaccaagg tggagaccag cgtcgaggcg
     5341 gttgcccaga ccgaaggccc gcgcgaagtg gaagtgcgcg cccgcgtcgg cggcatcctc
     5401 gtgaagcgcc tgtacgacga aggcgcggcg gtgaaggctg gccagcccct cttccagatc
     5461 gaccgggagc cctacgaggt ggccgtggcc caggccaagg cccaggtcgc cgaggccaag
     5521 gcccgcgtcg accagacccg ccgcgaagcc gcccgcctga agggcctcct cgcccagcag
     5581 gccatcagcc agcgcgaata cgacaccgcc acctctgacg atgccgccgc tgcggccgcc
     5641 gtgctggccg ccgaggcagc cctgcgccag gcccagctca atctggaata cgccaccgtc
     5701 accgcgccgg tggccggcct caccggccgc gtcgcggtgt cggaaggcaa tctgctggcc
     5761 accggcggcg ggacgctgct gaccacggtg gtccaggtga atcccatgtg ggtgcgcttc
     5821 tccctggcgg aggccgacct ggccgccgcc acgccgggcg gacgcttcaa ccccaaggcc
     5881 gtgcgcggcg tggaactggt cctgccggac ggcagcacct acccggtgcg cggccatctg
     5941 aacttctccg ccagccaggt ggatcccaag ctcggcaccc tgcagctgcg cggcgagttc
     6001 gccaacccgg acaacgtgtt gctgcccggc cagttcgtgc gcgcgcgcct gctcaccggc
     6061 gagcgcgacg gcgtcttcaa ggtgccccag aacgccgtga tgcagacgga catgggcgcc
     6121 gtggtgatgc tcgccaacgc cgacaacaag gtggagccgc ggccggtcaa gaccggcaac
     6181 tggagtggca aggactgggt gatcctgggc ggcctgaagg ccggcgacaa ggtcattgtg
     6241 gacaacctga tcaagatccg ccccggtgcg ccggtggcgc cccacgggcc gggcgaaggt
     6301 ccgggcgcgc cgggcggcca gccgggtgcg cctgccgccg cgccggccaa gggctgagga
     6361 ggcgcatccc atgtcacgct tcttcatcag tcgcccgatc ttcgcgtcgg tcatctccat
     6421 catcatcgtg atcgccggcc tgatggcctc cctcacgctg ccggtggccc agtaccccga
     6481 gatcacgccg cccaccatca tcatcagcgc cagctacccc ggcgccaacg ccgaaaccct
     6541 gtccaagacg gtggccgcgc ccatcgagga gcagctctcc ggcgccgagg ggctgatcta
     6601 ctacaactcc cagagcacct ccaacggcac ggtgaccatc accgccacct tcgaggtggg
     6661 caccaacccg gacaacgccc tcatcgcggt gaacaaccgg gtcaaggtgg ccgagccgcg
     6721 cctgcccgac gacgtgcgcc gcaccggcgt gctggtgcag aagcgctcca acaacatcct
     6781 gatgttcgcc gcggtgcgct cccccaaggg cgcgttcgac acgctctacc tgtccaacta
     6841 cgtagccctc aacatcatcg aggagatccg ccgcattccc ggtgtgggcg acgcccagca
     6901 gttcgacgcg atctacgcca tgcgggtgtg gctgaagccg gacgtgatgg ccaagctggg
     6961 catcaccacc accgacatcg ccaacgcgat ccgggtgcag aacacccaga acgccgccgg
     7021 caagatcggc caggagccgg tggtggatgg ccagcagctc acctacacgg tgacggccaa
     7081 gggtcgcctg ctcacgcccg aggagttcgg cggcatcatc ctcaaggccg aaggccccaa
     7141 cggcatcgtg cgcatccgcg acgtggcgcg catcgagctg ggcgccgaga actacgaccg
     7201 ctccaccgcg gtgaacggcg cgccggtgtc gggcatgggc atctacctgc agtccggcgc
     7261 caacgccctc gacacggcca agcgcgtgcg cgcgcgcctg gacgagatgc agaagcgctt
     7321 cccgcagggc atggaatact tcatccccta cgacaccacc cgcttcatcc aggagtccgc
     7381 ccacgaggtg gtcaagaccc tggtggaggc ggccctgctg gtgatcgcgg tggtctacct
     7441 gttcctgcag agctggcgcg ccaccctgat cccgatggtg gcggtgccgg tgtcgctgat
     7501 cggcaccttc gccgggatgt ggctgttcgg cttctccatc aacaccctga ccctcttcgc
     7561 catggtgctg gccatcggca tcgtggtgga cgacgccatc gtggtgctgg agaacgtgga
     7621 acgcctgatg agcgagcaga agctggcgcc cttcgacgcg gccgtggagg ccatgcgcga
     7681 ggtgtccggc gcggtggtcg ccatcgtgct ggtgctgtgc gcggtgttcg tgccggtggc
     7741 cttcctgggc ggcattgcgg gcaagctcta ccagcagttc gcggtgacgg tggcggtggc
     7801 ggtggtgatc tcgggcatcg tcgccctgac cctcacgccg gccctgtgcg ccctgctgct
     7861 caaggccagc cacgaggaga agccgctctt caagcccttc aaccggctct tcgagcgctt
     7921 cacccgcagc tacaccaaca ccgtcggcct gaccctgcgc cacggcatca tcggcaccct
     7981 ggtgttcctg ggcacgatcg gcgcgacggc cttcctgctg cgcatcgtgc cgggcagctt
     8041 cgtgcccgcc gaagaccagg gctacctgtt cggcttcgtc accctcaccg acggcgcctc
     8101 ggcccagcgc accttcgccg cctcggagca gatgcgcacg cggatcatgt ccgatgacat
     8161 cgagaacatc ttcttcgtga atggcatcga cttcatcacc ggcaacaacc gcgccagcgt
     8221 ggccaccacc ttcatcatct tcaagccctg ggagcagcgc agcgccaccg ccgacgacat
     8281 ggccaagaag ttcatgggcg tgggcatgag cctgtccgac ggcctcgggc tggtgttcaa
     8341 cccgccgccg atccagggcc tgggcaccgc cggcggcttc gaggtgtacc tgcagaaccg
     8401 cgccgacggc gacgcccgcc gcctcgccca ggtctccggc cagttcctcg aagccctcag
     8461 gaagcgtccc gagctgaccg gcctgaacag cttcttccgg gtgacggcgc cgcagctcta
     8521 cgtggaggtg gatgagccca aggccctgtc catgggcatc tccctcgaca gcatctacgc
     8581 caccctgcag gccaccaccg gcacgctgta cgtgaatgac ttcaaccgcg ccggcaagac
     8641 ctacaaggtc gagctgcagg ccgaggcagc ctaccggatg aacccggaag acctgctcaa
     8701 gccttacgtg cgcacggcgt ccggtgcgat ggtgcccctc tctgccgtcg ccaaggtgaa
     8761 gaccctcacc ggcgccgaga tggtggagcg cttcaacggc ttcgtctccg ccaaggtgct
     8821 gggcaacgcc gcgccgggct tcagctctgg ccaggccatc caggccgtgg aagaagtggc
     8881 cagggaagcc ctgcccgaag gcttccaggt ggaatgggtg ggccaggcct tccaggagaa
     8941 gcgcaccggc tcggcctcca tcgtggcctt caccttcggc atcatcatgg tgttcctgat
     9001 cctggccgcc cagttcgaga agtggtccct gcccctggcg gtgatcatgg cggtgccct
//
LOCUS       DAOQPA010000129         8821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4307_length_8821_cov_19.143395, whole genome shotgun sequence.
ACCESSION   DAOQPA010000129 DAOQPA010000000
VERSION     DAOQPA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4307_length_8821_cov_19.143395"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..913)
                     /locus_tag="PKX01_14535"
     CDS             complement(209..913)
                     /locus_tag="PKX01_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77397.1"
                     /translation="MLPSRPPLRLSVVIVLSLLAGIARAADATPDPAAELARARALRE
                     QAGTLRDAAEARFDDENAACFKRFLVNRCIDQAKQRRLADVRKARDMDLEADRVELAD
                     KNRRFNAHQADKDETAARDALERAQKEAEARREYEARMKTFADKDAERVRREQEGHFQ
                     AIQEAEARSRKDAEDARRRAEEAAAATARADKARQDREHYAEEARKHAEKMAEKAAKK
                     EAEAASGKPAPKPPGF"
     gene            1015..2946
                     /locus_tag="PKX01_14540"
     CDS             1015..2946
                     /locus_tag="PKX01_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ77398.1"
                     /translation="MIQFRNLRLARGVKTLVEGASLQIHPGWKVGLTGANGCGKSSLF
                     GLLRGELHPDAGDLELPAAWVIAHVAQETPALPRPAIEYVLDGDAELRRIETALTAAE
                     AAHQGEEIGHLHGRLQEIGGYAARARAAALMDGLGFGSADLERPVADFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLDAVIWLEQWLTDYRGTLILISHDRDFLDAVVNQIAH
                     IEGQRLTLYAGGYSDFERQRAERLSQQQALFDKQQRERAHLQKYVDRFRAKATKARQA
                     QSRIKALERMELISAAHVDTPFSFSFRACGAAPDPLLQIEDAAAGYGAAPLLADMSLT
                     IRPGERIGLLGRNGAGKSTLIKLLSGELAPLTGVRREGKGLALGYFAQHALERLRPDE
                     SPLQHMLRADPQTREQELRDYLGGFDFRGDGTSSAGAGATTPCGPFSGGEKSRLALAL
                     LIWGRPNLLLLDEPTNHLDLEMRHALTLALQDYEGGMVIVSHDRALLRATCDRFLLVD
                     GGRLTPFDGDLDDYKAWLAQRRSADAAAAQCPDRAADKASRKAEREAAAADRQARLAA
                     RRPLLKELERLEKQLTPWTAEKASLDERLADPALYTGPDAGEVPKLIKRQAELADRLD
                     AAELRWLELHEALEAIPAD"
     gene            3122..3730
                     /gene="thrH"
                     /locus_tag="PKX01_14545"
     CDS             3122..3730
                     /gene="thrH"
                     /locus_tag="PKX01_14545"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HMZ77399.1"
                     /translation="MQLVCLDLEGVLVPEIWIEFAERTGIPELRRTTRDEPDYDKLMK
                     YRLDLLKQHKLGLPDIQKVISEMGPEPGAKDFLDALRQDFQVIILSDTFYEFAMPLMQ
                     QLGMPTLFCHKLEANAEGYLVNYHLRMANQKKEAVQRFKEINFKVIAAGDSYNDTAML
                     KEAHAGILFRAPDNVIAEFPQFPVEREYSGLMAQIRAASARI"
     gene            3780..4655
                     /gene="purU"
                     /locus_tag="PKX01_14550"
     CDS             3780..4655
                     /gene="purU"
                     /locus_tag="PKX01_14550"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HMZ77400.1"
                     /translation="MHRERFYTLSASCPDRTGIVAKVSGFIAQHKGWILETALHAEPA
                     TDAEHRGRYFMRIEIRADSLPFMLTEFRERFAPIAEELQMDWKITDSAVKKRVVIMVS
                     KQEHCLYDLLARWQSKELDIEIPCVISNHDTFRGFVEWHGIPFHHVPVTADNKPRAFA
                     EVQRIFEEVHGDTMVLARYMQILSPDLCAAHPGRIINIHHSFLPSFVGAKPYHQAYDR
                     GVKLIGATCHYVTADLDQGPIIEQDVIRIDHSDSVEDMVRYGKDIEKAVLARGLRYHL
                     EDRVLVHGNKTVVFR"
     gene            4876..5487
                     /locus_tag="PKX01_14555"
     CDS             4876..5487
                     /locus_tag="PKX01_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77401.1"
                     /translation="MRDFLDKVRTLRWDDHRYYHQSRINQTLHLISAISFLVAYGLLF
                     SDPALAGLVGWLVGMVTRQSGHIFYEPRGFDAIHNTTYAHKESIKVGYNMQRKGVLIG
                     VWLSIPVLLLVSPDAFGLLDAGGGAAGYLHNLGLLWLCLGAGGLGFRTLHLFFLRGPV
                     WGLAWAFKILTDPFHNIDIYWRSPIALMRGQLLDPLDGEEAHA"
     gene            complement(5582..7249)
                     /locus_tag="PKX01_14560"
     CDS             complement(5582..7249)
                     /locus_tag="PKX01_14560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77402.1"
                     /translation="MFRSPARSPRWAAVIVALVLLVPPLHASAREAVTLQLKWRHAFQ
                     FAGYYAAKEKGFYDAAGLDVHLQEATPGMDPVEPVLNGQAQYGVGNSSLLLARRAGQP
                     VVVLAVVFQHSPLVLLTRQQPDTVDAQGLQHLAGKRVMIEPHSDELIAYLKREGVPLD
                     QLVQVPHSYRPEDLIEGRVDAITAYASNEPFYLDRAGLAYQSYSPRAAGIDFYGDNLF
                     TTEDELRNHPDRVRAFRDASLRGWHYALEHPTEIADLLHRHYAPQHPRAFFLFEAQRM
                     MPLLRADLIEVGYMNPERWRHIADTYADLGLLPRDYALDGFLYAPDVQLDRSRFYLTL
                     ALLALVTGAAMYVVRINRRLSRVLAESQAAEARIRHLAQHDPLTELPNRALLSDRLGQ
                     ALVAAKRDTRRLAVMFIDVDNFKPINDTLGHAVGDRLLQQIAKRLQASLRESDTVARI
                     GGDEFVVLLRNVTDGPDALAVADKLREVLSVPIVVEAHTLVVGASVGVALYPDHGSDA
                     LELFRHADEAMYRAKRDGRNRAQVFEARSDRGQPLAPSQPAASSVGP"
     gene            complement(7400..>8821)
                     /gene="amt"
                     /locus_tag="PKX01_14565"
     CDS             complement(7400..>8821)
                     /gene="amt"
                     /locus_tag="PKX01_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ77403.1"
                     /translation="PAEAKPAEAPAAEAKPAEAAPAAAAEAAPAEAAAAPVPPNKGDN
                     AWLIVATAFVILMSIPGLALFYGGLVRAKNMLSVLLQVFTVFSLISVLWVLYGYSVAF
                     TEGNAFFGGLDKVFLKGITPDSVAATFSKATNISEFIYVAFQGAFAAITVALILGAFA
                     ERIKFSAVLLFSVLWFTFSYLPIAHMVWFWAGPDAYTSAEAADAANATAGWLFQKGAL
                     DFAGGTVVHINAAVAGLVGAFLVGKRIGFGRESFAPHSLTLTIVGASLLWVGWFGFNA
                     GSALEANGVAALAFVNTWVATAAAAMSWLLAEWIIKGKPSGLGAASGAVAGLVAITPA
                     CGFVGVVGAIVIGLLAGVVCLWGVNGLKRMLGADDSLDVFGVHGVGGILGATLTGVFA
                     APSLGGSGIWDYVANKAADAYSIGDQVVIQLTAVGTTIVWSAVVAFISYKLVDMFIGL
                     RVPEDEEREGLDVTSHGETAYHQ"
BASE COUNT         1423 a         3025 c         2996 g         1377 t
ORIGIN      
        1 ccggctggag gatgcgctga gctgggtgaa ggacgggccg acgctgtagg cgggcggggc
       61 cacgagcctc ggcgcgctgt cgaccgcggg gaaccccgag gagccgggcg cgagtcgggg
      121 cgaaccggcg ggcgcggtcc aaacccggct gcaggggcat cacctgcggg gtcgcgtggc
      181 ggcccgatga gatccggatt tgccgccctc agaaccccgg cggcttcggc gccggtttgc
      241 cggaggcggc ctcggcctcc ttcttggcgg ctttttccgc catcttttcg gcgtgcttgc
      301 gcgcttcctc ggcgtagtgt tcccgatcct ggcgggcctt gtcggcgcgg gccgtggcgg
      361 cagccgcctc ttcggcgcgg cggcgggcgt cctcggcgtc cttgcggcta cgggcctcgg
      421 cttcctggat ggcctggaag tggccttcct gttcgcggcg gacgcgctcg gcgtccttgt
      481 cggcgaaggt cttcatgcgc gcctcgtatt cccgacgcgc ctcggcttcc ttctgggccc
      541 gctccagggc atcgcgggcg gcggtctcgt ccttgtcggc ctggtgggca ttgaagcggc
      601 ggttcttgtc agccagttcg acgcggtcgg cctccaggtc catgtcccgc gccttgcgca
      661 cgtccgcgag gcggcgctgc ttggcctgat cgatgcaacg attgaccagg aaacgcttga
      721 agcaggcagc gttttcgtcg tcgaagcgcg cctcggcggc atcccgcagg gtgccggcct
      781 gttcccgcag ggccctggcg cgcgccagct cggctgctgg gtcgggcgtc gcgtcggcag
      841 cgcgggcgat tcccgccagc aggctcagga cgatcacgac ggacaggcgc aggggcgggc
      901 ggctcggcaa catgacggcg atacgggggt cgggcgggga cgggaccgct tacaataccg
      961 gatttcctgc cggcgcgcct gcccggcccc accgattcga ccccttccca cgccgtgatc
     1021 cagttccgaa acctgcgcct cgcccggggc gtgaagaccc tcgtcgaagg cgccagcctg
     1081 cagatccacc ccggctggaa ggtcggcctc accggcgcca acggctgcgg caaatcgagc
     1141 ctgttcggcc tgctgcgcgg cgagctgcat cccgacgccg gcgacctgga gctgcccgcc
     1201 gcctgggtga ttgcccacgt ggcccaggag acgcccgccc tgccgcgccc ggccatcgag
     1261 tacgtgctcg acggcgacgc cgagctgcgc cgcatcgaaa ccgccctgac cgccgccgag
     1321 gctgcccacc agggtgagga gatcggccat ctccacggcc gcctgcagga gatcggcggt
     1381 tacgccgccc gcgcccgcgc tgccgcattg atggacggtc tgggcttcgg cagcgccgac
     1441 ctggagcggc cggtggcgga tttctccggc ggctggcgca tgcgcctcaa cctcgcccag
     1501 gccctgatgt gccgctccga cctgctgctg ctggacgagc ccaccaacca cctggacctg
     1561 gacgcggtga tctggctgga gcagtggctg acggactacc gcggcacgct gatcctcatc
     1621 tcccacgacc gggacttcct cgatgcggtg gtgaatcaga tcgcccacat cgagggccag
     1681 cgcctcaccc tctacgccgg cggctactcg gatttcgagc gccagcgcgc cgagcgcctg
     1741 tcccagcagc aggccctctt cgacaagcag cagcgcgagc gcgcccacct gcagaagtac
     1801 gtggaccgct tccgcgccaa ggccaccaag gcgcgccagg cccagagccg catcaaggcc
     1861 ctggagcgca tggagctgat ctccgcggcc cacgtggata cgcccttcag cttcagcttc
     1921 cgggcctgtg gcgcggcacc cgatccgctg ctgcagatcg aggacgccgc cgccggctat
     1981 ggcgcggcgc cgctgctcgc ggacatgtcg ctgacgatcc ggccgggcga gcgcattggc
     2041 ctgctgggac gcaacggcgc cggcaaatcc accctcatca agctgctgtc gggcgagctg
     2101 gcgcccctca ccggggtgcg ccgcgaaggc aaaggcctgg ccctcggcta cttcgcccag
     2161 cacgctctgg agcgcctgcg cccggacgag tcgccgctcc agcacatgct gcgcgccgac
     2221 ccccagaccc gcgagcagga gctgcgcgac tacctgggcg gcttcgactt ccgcggcgat
     2281 ggcacgagca gcgccggggc cggggcgacc acgccctgcg ggcccttctc cggcggcgag
     2341 aaatcgcgcc tcgccctggc cctgctgatc tggggccggc ccaacctgct gctgctggac
     2401 gaacccacca accacctgga cctggagatg cgccacgccc tgaccctggc gctccaggac
     2461 tacgagggcg gcatggtaat cgtgtcccac gaccgggccc tgctgcgcgc cacctgcgac
     2521 cgcttcctgc tggtggacgg cggtcgcctg acgcctttcg acggcgacct ggacgactac
     2581 aaggcctggc tcgcccagcg ccgcagcgcc gacgcggccg ccgcccaatg tcccgaccgg
     2641 gccgccgaca aggccagccg caaggccgag cgggaagccg ccgctgccga ccgtcaggcc
     2701 cgcctcgccg cccgccggcc gctgctgaaa gagcttgaac gcctggaaaa gcagctcacg
     2761 ccgtggaccg ccgaaaaggc gagcctcgac gaacgccttg ccgacccggc gctgtacacc
     2821 ggcccggacg cgggcgaggt gcctaagctg atcaagcgcc aggccgagct ggccgacagg
     2881 ctcgacgccg ccgagctgcg ctggctggag ctccacgagg cgctggaagc gattccggcg
     2941 gattgacgcg cccggcgccc gcctcgttcc cgtctccacc gccctggccc gcgcgggcgc
     3001 gtccggggtg tgcaggtggc cggcgccgcg cgccgcctgc cacagacttc ctcaaggcgt
     3061 ctccgtgtct ttgctagaat cgcggcttcg aaatcaaccg tcgtactctg gagtccacgc
     3121 cgtgcaactc gtctgtctcg acctcgaagg tgtcctcgtc cccgaaatct ggatcgaatt
     3181 cgcggagcgc accggcattc ccgaactgcg tcgcaccacc cgcgacgagc cggactacga
     3241 caaactcatg aagtaccgcc tggatctgct caagcagcac aagctgggtc tgccggacat
     3301 ccagaaggtc atctccgaga tgggccccga gcccggtgcc aaggacttcc tcgacgccct
     3361 gcgccaggat ttccaggtga tcatcctgtc cgacaccttc tacgaattcg ccatgccgct
     3421 gatgcagcag ctgggcatgc cgaccctgtt ctgccacaag ctggaggcga acgccgaggg
     3481 ttacctggtg aactaccacc tgcgcatggc caaccagaag aaggaagcgg tgcagcgctt
     3541 caaggaaatc aacttcaagg tcatcgccgc cggcgattcc tacaacgaca ccgcgatgct
     3601 caaggaagcc cacgccggca tcctcttccg cgccccggac aacgtcatcg ccgagtttcc
     3661 ccagttcccc gtggagcgcg agtacagcgg cctgatggcc cagatccgcg ccgcctccgc
     3721 ccgcatctga cccttctgca gcgccgcctc gcgcccggcg cggggggcgc gagaccatca
     3781 tgcaccgcga acgcttctac accctgtccg cctcctgccc cgaccgcacc ggcatcgtcg
     3841 ccaaggtttc gggcttcatc gcccagcaca agggctggat cctcgagacc gccctccacg
     3901 ccgaaccggc gacggacgcc gagcaccgcg gacgctactt catgcgcatc gagatccgcg
     3961 cggattcgct gcccttcatg ctcaccgagt tccgcgagcg cttcgcgccc atcgccgaag
     4021 aattgcagat ggactggaag atcaccgatt cggcggtcaa gaagcgcgtg gtgatcatgg
     4081 tctccaagca ggagcactgc ctctacgacc tgctcgcccg ctggcagtcg aaggagctgg
     4141 acatcgagat cccctgcgtg atctccaacc acgacacctt ccgcggcttc gtggaatggc
     4201 acggcatccc cttccaccac gtaccggtga cggcggacaa caagcctcgc gccttcgccg
     4261 aggtgcagcg catcttcgag gaagtccacg gcgacaccat ggtgctggcg cgttacatgc
     4321 agatcctcag cccggacctg tgcgccgccc atccgggccg gatcatcaac atccaccaca
     4381 gcttcctgcc cagcttcgtc ggcgccaagc cctatcacca agcctacgac cggggcgtca
     4441 agctgatcgg cgccacctgc cattacgtca ccgccgacct ggaccagggc cccatcatcg
     4501 agcaggacgt gatccgcatc gaccattccg actcggtgga ggacatggtc cgttacggca
     4561 aggatatcga gaaggcggtc ctcgcccgcg gcctgcgtta tcacctggaa gaccgggtgc
     4621 tggtccacgg caacaagacg gtcgtgttcc gctgagccgt ccgccgtcag acccgtgtga
     4681 gccggcccgc gtggccggct ttttcatggc cgcggcgccc gatgtgacgg tgacgtggcg
     4741 cgtgcgtccc gaaatgttga cctggctcaa acgacttctc cgtgacgcaa atctgtaacg
     4801 cctccattac cggagcgtca tgcaagcccg tcacattgcg gtcacgcaaa gcattcatta
     4861 ccgggagcct tcaacgtgcg tgactttctc gacaaggtgc ggaccctccg ctgggacgac
     4921 caccgctatt accaccagag ccgcatcaac cagaccttgc acctgatcag cgcgatcagt
     4981 tttctcgtgg cctacggcct gctgttctcc gacccggccc tggccgggct ggtgggctgg
     5041 ctggtgggca tggtgacgcg ccagtccggc cacatcttct acgagccgcg cggcttcgac
     5101 gccatccaca acaccaccta cgcgcacaag gaatccatca aggtgggtta caacatgcag
     5161 cgcaagggcg tgctgatcgg cgtgtggctg tccatcccgg tgctgctgct ggtgagtccc
     5221 gacgccttcg ggctgctcga cgcgggcggc ggcgcggccg gctacctcca caacctgggt
     5281 ctgctgtggc tgtgcctcgg cgccggcggg ctgggcttcc gcaccctgca cctgttcttc
     5341 ctgcgcggac cggtctgggg actcgcctgg gctttcaaga tcctgaccga tccgttccac
     5401 aacatcgaca tctactggcg cagcccgatt gcgctgatgc gtggtcagtt gctcgatccg
     5461 ctggacgggg aggaggccca cgcgtgagca gcggggcctg gacgaccggc gcgccgcggc
     5521 atgcggcgca gtccggcgtt cggctggatg gcgggcgccc ggtggggcgc ccgttgcacg
     5581 tttacgggcc gacgctcgaa gccgcaggct gcgacggcgc caggggctgc ccccggtcgc
     5641 tacgggcttc gaacacctgt gcccggttgc gaccatctcg tttggcgcga tacatggctt
     5701 cgtcggcgtg ccggaacagt tcgagcgcat cgctgccgtg gtcggggtac agggccacgc
     5761 cgacgctggc accgacgacg agggtgtgtg cctccaccac aatgggtacg gagaggactt
     5821 cgcggagttt gtccgccacg gcgagggcgt ccggcccgtc cgtgacgttg cgcaggagca
     5881 ccacgaattc atctccaccg atgcgggcca cggtgtccga ttcccgcagg ctggcctgca
     5941 ggcgcttggc gatctgctgt aacaggcgat cgcccaccgc gtgccccagg gtgtcgttga
     6001 tgggtttgaa gttgtccacg tcgatgaaca tcaccgccag gcgccgggtg tcgcgtttgg
     6061 cggccaccag ggcctggccc aggcggtcgg acaggagggc acgattgggc agctcggtca
     6121 gtgggtcgtg ctgggccagg tgccggatgc gggcttcggc ggcctgactt tcggccagca
     6181 cgcgggacag gcgccggttg atgcggacca cgtacatggc ggcgcccgtg accagcgcca
     6241 gcaaggccag ggtcaggtag aagcggcttc ggtcgagctg gacgtccggt gcgtagagga
     6301 agccgtcaag ggcgtagtcg cggggcagca ggcccagatc ggcgtaggtg tcggcaatgt
     6361 ggcgccagcg ctccggattc atgtagccca cctcgatgag gtcggcgcgc agcagcggca
     6421 tcatgcgctg ggcttcgaaa agaaagaagg ctcgcgggtg ctggggcgcg tagtggcggt
     6481 ggagcaggtc ggcaatctcg gtcgggtgct ccagggcgta atgccagcct ctcaggctgg
     6541 cgtcccggaa ggcgcggacc cgatccgggt ggttgcgcaa ttcgtcttcg gtggtgaaga
     6601 ggttgtcgcc gtagaagtcg atgccggcgg cccggggcga ataggattgg taggcgaggc
     6661 cggcgcggtc gaggtagaag ggctcattgc tggcgtaggc cgtgatggca tcgacccggc
     6721 cttcgatcag gtcctcgggc cggtagctgt ggggcacctg gaccagctgg tcgaggggaa
     6781 caccctcccg tttcagatag gcgatcagtt cgtcggaatg gggttcgatc atcacccgtt
     6841 tgccggcgag gtgttgaagg ccttgcgcgt ctaccgtgtc gggttgctgt cgcgtcagca
     6901 gcaccagggg tgaatgctgg aacaccacgg ccagcacgac caccggctga ccggcccggc
     6961 gtgccagcaa caggctgctg ttgcccacgc catactgggc ctgaccgttg aggaccggct
     7021 cgaccggatc catgccgggt gtggcttcct gcagatgcac gtccaggccg gcggcgtcgt
     7081 agaacccctt ttccttggcc gcgtagtagc cggcaaactg gaaggcgtga cgccatttga
     7141 gctgcagggt caccgcctcc cgcgccgagg cgtgcagggg cggaaccagc aggaccagcg
     7201 cgacgatgac agccgcccag cgaggcgatc tggcggggga acggaacatc gcaccattgt
     7261 acgccgatgg catttatccg gtgcgtccat ggagcgcgtg tctggaatgg acccgggcgc
     7321 gtgcgtctgc ccaggcgcgc cgaacaaatg aaaacgggcg ccgaagcgcc cgttttgccg
     7381 acggaacctg ttaccgatgt tactgatggt aagcggtttc gccgtgggag gtgacgtcca
     7441 gaccttcgcg ctcttcgtct tccggcacgc gcagaccgat gaacatgtcg accagcttgt
     7501 aggagatgaa ggccaccacg gcggaccaca cgatggtggt gccgacggcg gtcagctgga
     7561 tcacgacctg gtcaccgatg gagtaggcgt cggcggcctt gttggccacg tagtcccaaa
     7621 tgccggagcc gcccagggag ggggccgcga acacgccggt cagggtggcg ccgaggatgc
     7681 cgcccacgcc gtgcactccg aagacgtcca gggagtcgtc ggcgccgagc atacgcttga
     7741 gaccattgac accccacagg cagaccacgc cggcaagcag accgatcacg atggcaccga
     7801 ccacaccgac gaagccgcag gcgggggtga tggccacgag gccggccacg gcaccggaag
     7861 cagcgcccag gccagagggc ttgcccttga tgatccattc ggccagcagc caggacatgg
     7921 cggcggcagc ggtggcaacc caggtattca cgaaggccag ggcggcgacg ccgttggctt
     7981 ccagggcgga accggcgttg aagccgaacc agcccaccca cagcagggag gcgccgacga
     8041 tggtcagggt caggctgtgg ggcgcgaagg attcacgacc gaagccgatc cgcttgccca
     8101 ccaggaaggc acccaccagg ccggccacgg cggcgttgat gtgcaccacg gtgccgccgg
     8161 cgaagtcgag ggcgcccttc tggaacagcc agccggccgt cgcgttggcc gcatcggccg
     8221 cttcggcgga ggtgtaggcg tccggaccgg cccagaacca caccatgtgg gcgatgggca
     8281 ggtagctgaa ggtgaaccac agcacggaga acagcagcac ggccgagaac ttgatgcgct
     8341 cggcgaaggc gccgaggatc agggccacgg tgatcgccgc gaaggcgccc tggaaggcca
     8401 cgtagatgaa ctcggagatg ttggtggcct tgctgaaggt ggccgccacg gaatccggcg
     8461 tgatgccctt caggaagacc ttgtccagac cgccgaagaa ggcgttgccc tcggtgaagg
     8521 ccacggagta gccgtacagc acccacagga cgctgatcag cgagaacacc gtgaacacct
     8581 gcaggagcac cgacagcatg ttcttggcac ggaccaggcc gccgtagaac agggccaggc
     8641 cagggatgga catcaggatg acgaaggcgg tggcgacgat cagccaggcg ttgtcgccct
     8701 tgttgggggg caccggcgcg gcagcggctt cggccggggc ggcttcggca gcggcagcgg
     8761 gggccgcttc ggcgggcttg gcttcggcgg cgggggcttc ggccggcttg gcctcggcgg
     8821 g
//
LOCUS       DAOQPA010000130         8763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4348_length_8763_cov_19.331764, whole genome shotgun sequence.
ACCESSION   DAOQPA010000130 DAOQPA010000000
VERSION     DAOQPA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_8763_cov_19.331764"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..381
                     /locus_tag="PKX01_14570"
     CDS             211..381
                     /locus_tag="PKX01_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77404.1"
                     /translation="MKRLLALALIAASLGGCVVLPPERGPRYMGPPPVVVAPGRPYHY
                     YDHDRDWGYRRW"
     gene            complement(395..1249)
                     /locus_tag="PKX01_14575"
     CDS             complement(395..1249)
                     /locus_tag="PKX01_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77405.1"
                     /translation="MNESLQSWWFFLCAAATVNVLAWLMSAAMLRRGGPDLPERARAL
                     RRTQLLLSAGYVFGCAFRSVLPVFDVPRLCLVDSWLSSVVVGRSVATVAELCFVAQWA
                     LILREVARATHASFARRAAALMVPLIAVAETCSWVAVLTTANLGHVIEESLWGLSALL
                     FVLGVLETRPRCPPWLHGRLLLWGAAGLAYTVFMFGVDVPMYWSRWIADEHVGRAYLS
                     LTQGLTDASTRWVVSHRWEDWRSEVVWMSLYFSVAVWFSIALVHTRLPARVRLHSRSL
                     RSPAASRA"
     gene            complement(1329..2219)
                     /locus_tag="PKX01_14580"
     CDS             complement(1329..2219)
                     /locus_tag="PKX01_14580"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HMZ77406.1"
                     /translation="MICPSLPDTPSGFPLAIIGAGALGLHFAARLAGCGPVAVLARSA
                     ARAETLRAGLVVGDARFRPQAFGPDDPLRAEWVLLLVKTGDTEAAARQALHLQPHGIL
                     SLQNGLTESRLRAVCGSVPAAQGLTTEGAYRDSAGVHPAGAGETLLPPGFETLAAHLQ
                     AAGFRARIEPDMGPARLAKLLVNLAINPLTALHRVPNGALLDAPYRVELEALVDEAWP
                     VLHAAGLRLDRTAAHARVVTVAAATAGNRSSMLQDVLAGRPTEIDAITGAVLDMASAQ
                     GRDLPLNRTVYTRIKGLAPP"
     gene            2389..3675
                     /locus_tag="PKX01_14585"
     CDS             2389..3675
                     /locus_tag="PKX01_14585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77407.1"
                     /translation="MATQGDEFPAEVGFAAIFAASPDGLLVVDGSGRIRLANPAVEHM
                     FGYAAGELIGQAIEVLVPAHAAGHHVRDRMAYAAAPRTRPMGASTELAGRCRDGSLVP
                     VDIMLAPVDATGTRGTLCVVRDARPRHATEAKLHLASAVFQTTQEAIVVTDTQCRILA
                     VNPAFERVTEYAEAEVLGQHMRIVASGRHDRSFYDAMWTSIHTTGEWQGAIWNRRRSG
                     EIYQEWLSISTVRDREGRAFQYVGVSADMTRMNHAETPIERLAHFDPLTGLPNRLLLD
                     SRIRKTWERAHRDGLRFAVLFLDLDGFKAVNDDRGHAVGDELLRVIAARLAGSVRESD
                     TVARLGGDEFIVVLEDLRREDVARLADKLIARVAEPVELSDGSQAHVGLSLGWSTYPD
                     DAQDIDTLMGQADTALYRAKRGGRGRAVAFVGSGPA"
     gene            complement(3686..6013)
                     /locus_tag="PKX01_14590"
     CDS             complement(3686..6013)
                     /locus_tag="PKX01_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ77408.1"
                     /translation="MIGLSERLQALRGRLRRPTRRELLRALVALPLLFVLWVLVLIPF
                     TPGIGDLRKAKQERPARVLSADGREIAVFKWANREWVGLADISPHVVKALIATEDHRF
                     YEHHGIDFYRLGGALLHTFSGDRQGGSTITQQLARNLYPDEIGRAPTLTRKLREAITA
                     LKIEAVYSKDEILETYLNTVPFLYNAWGIEMAARTYFDHSARQLDILESATLVGMLKG
                     NSAYNPVQNPERAVARRNVVLAQMARRGQLDPARLDALRKKPLRIDFERQVEAPGPAP
                     HFAQQVRKWLIAWADRNGYNLYADGLVVRTTLDSRLQVDATRAVARRGRQLQALADQA
                     WSGRKGWSGSPELVAALVRESRDYRNAVAAGQAPDAVLARLLRDTVFLNALRADKTRI
                     QAGLLAIDPRDGQVRAWVGSRDFVQDPFDHVQQARRQPGSTFKPFVYGAAFARGARPE
                     DTLDDVAVEIPVRGDEVWRPTDESPPSGRPVSLRDGLAYSRNRITAQLMQQVGPARVA
                     HLARALGVRQSPLEEVPSLALGTSPVTLREMVTAYAAIADGGHYREPLLVTQVEDADG
                     HILERIAPPAPEEALDPAVAYTLLDTLRGVIDRGTGNALRSRFGLRADLAGKTGTTQD
                     NTDGWFIAMHPQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPIVGDLLHDAVAERLVD
                     GKARFVSQARPGLLTRAWDWLRDLFDRPEAPPAPPRKAPRAAPPAETPPPPPVGPELP
                     PPEDLAPPPAAAPAAAVPVPDTAPLPPPAEASARP"
     gene            complement(6042..7562)
                     /gene="ilvA"
                     /locus_tag="PKX01_14595"
     CDS             complement(6042..7562)
                     /gene="ilvA"
                     /locus_tag="PKX01_14595"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HMZ77409.1"
                     /translation="MSAQHPDYLQKILTAQVYDAAIETPLDLAPNLSARIHNRIFFKR
                     EDMQPVFSFKLRGAYNKMASLSPAALKRGVICASAGNHAQGVALSARKLGVRAVIVMP
                     TTTPAIKIDAVRRHGGEVVLAGESYSDCYLHAKELEKAEKLTFVHPYDDVEVIAGQGT
                     IGMEILRQHPKPIHAVFCCVGGGGLLAGVAAYIKRVRPDIKVIGVETVDANAMTQSLA
                     AGKRIELANVGLFADGAAVKLVGEETFRLCQAYVDEMILVDNDAICAAIKDVFEDTRS
                     ILEPAGALAVAGAKEYAKRHALKDKTLVAVASGANMNFDRLRFVAERAEVGEQREAVL
                     AVTIPEKPGSFKKFCTLLGSRNITEFNYRYADAHRAHIFVGVGVHNRAEGVRLTELLH
                     RHELPCIDLTDDEMAKSHIRYMVGGHAPQATNEVLYRFIFPERPGALLNFLETLRSDW
                     NISLFHYRNHGADFGRVLVGMQVPPADRDAFQGFLDKLGYEYVDETLNPAYRMFLG"
     gene            7655..8008
                     /locus_tag="PKX01_14600"
     CDS             7655..8008
                     /locus_tag="PKX01_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ77410.1"
                     /translation="MTPILRRGTTARYSDFTVYGGVVYCVEVPPAGAADASAQTAAML
                     ASLERLLVEAGSAKDRLLLATLYLTDMADYDAVNAVWDAWVPPGTAPARACVQVCRLA
                     DPAWKVEVAVQAARN"
     gene            8216..>8763
                     /locus_tag="PKX01_14605"
     CDS             8216..>8763
                     /locus_tag="PKX01_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ77411.1"
                     /translation="MARSFVSKLAGALLAAGLSLAGSAHAALFSNMYVFGDSLSDVGN
                     DLAITGGLVPDPAYYSNGAVTGRFVNGYNYADRLASQLGLSLAPSVLGGNDYAYGGAR
                     STYARPDLAVLGALSFQQQVNQYLGNVGGVVDPNALYVLWIGANDMSDGFAQTLAKLA
                     GTGVLDTSFIGNAAANALTTLFT"
BASE COUNT         1302 a         2807 c         3296 g         1358 t
ORIGIN      
        1 gggccggccg gcgtgtccac gtcggcgaga aagcgctggt aacggcggat caggcggcct
       61 tcgaggagcg gcttggtaaa gcgcatgggg cgtggggcgg cgggacgatc cgcccatcat
      121 gccagactta cacgcggtaa ccctttcatc ccaacctggc gccgggctcg tgcgttgaac
      181 gctcaccttc atcaccgacc ggagatcgtc atgaaacgcc tgctggccct tgccctgatc
      241 gctgcctcac tggggggctg cgtggtcctg ccgccggagc gcggcccgcg ctacatgggc
      301 ccgccgccgg tggtggtcgc ccccggccgg ccctaccact actacgacca cgaccgggac
      361 tggggttatc gccgctggtg agcggcttcg agcgtcaggc acgcgacgcg gcgggcgacc
      421 gcaggctgcg gctgtgcagg cgcacgcggg ccggcagacg ggtgtggacc agggcaatgc
      481 tgaaccacac cgccacgctg aagtagaggg acatccacac cacctcgctg cgccagtctt
      541 cccagcgatg ggacaccacc cagcgcgtgg aggcatccgt caggccctgg gtgaggctca
      601 ggtaggcgcg gcccacatgc tcgtcggcga tccagcgcga ccagtacatg ggcacgtcca
      661 cgccgaacat gaagacggtg taggccagac ccgccgcacc ccacaggagg aggcgcccgt
      721 gcagccaggg gggacagcgc ggacgggttt cgagcacgcc caacacaaag agcagggcgc
      781 tcaggcccca caggctctcc tcgatcacgt ggcccaggtt ggcggtggtc agcacggcga
      841 cccacgagca ggtttccgcc acggcgatca gcggcaccat cagcgccgcc gcccggcgcg
      901 cgaaggaggc atgggtggcg cgggccacct cgcgcaggat cagggcccac tgggcaacga
      961 agcacagctc tgccaccgtg gctacgctgc gccccaccac cacgctggac agccagctgt
     1021 ccaccaggca caggcgtggc acgtcaaaga ccggcagcac ggagcggaag gcacagccga
     1081 agacgtaacc ggccgacagg agcaactggg tccggcgcag ggcgcgggcc cgctcgggca
     1141 ggtccggacc gccgcggcgc agcatcgcgg ccgacatcag ccaggcgagc acgttgaccg
     1201 tcgcggcggc acacaggaag aaccaccagg actgcaggct ctcgttcatg atgcttcaag
     1261 ctaggtcgcc cgaagcgggc cgtcaaggca attcggtaat accgtatgcg ctttgtgcgg
     1321 cagcgtgctc agggcggggc aagccccttg atccgcgtgt agacggtccg gttgaggggc
     1381 aggtcgcggc cttgcgcgga cgccatgtcg aggactgcgc cggtgatggc gtcaatttcg
     1441 gtgggccgtc cggcgaggac gtcctgcagc atgctggagc ggttgccggc ggtggctgcg
     1501 gcgactgtta ccacccgcgc gtgggcggcg gtccggtcca gcctcagccc ggcagcgtgg
     1561 agcacgggcc aggcttcgtc caccagagct tccagttcga cccggtaggg cgcatccagc
     1621 aaggccccgt tgggaacccg gtggagcgcc gtgagcgggt tgatggcgag gttgacgagc
     1681 agcttggcaa ggcgggcggg gcccatgtcc ggctcgatgc gggcgcggaa gccggcggcc
     1741 tgcagatggg cggccagcgt ctcgaagccc ggtggcaaca gggtttcgcc tgcgccggcg
     1801 ggatgcacgc cggcgctgtc ccggtaggca ccttcggtgg tgagcccctg ggcggcgggc
     1861 acgctgccgc agacggcacg caggcgcgat tcggtgaggc cgttctgcag ggacaggatg
     1921 ccgtgcggct gcaggtggag ggcctgccgg gccgccgcct cggtgtcgcc ggtcttgacc
     1981 agcaggagca cccactcggc gcggagcggg tcgtccgggc cgaaggcctg gggccggaag
     2041 cgggcatcgc ccaccacaag ccccgcacgc agggtctcgg cccgggctgc gcttcgggcc
     2101 aggactgcga ccggaccgca gccggcgagg cgggccgcaa agtgcaggcc gagcgcaccc
     2161 gcgccgatga tggcgagggg aaatcctgat ggcgtgtcgg ggagggaagg gcagatcatg
     2221 gcgcgggcga tcgggggcga cggtccggat tgtcccccgc ccagcgcttc agtgggggtg
     2281 tccgggcagg ttccgaagca gttacagtag cggtccaggt gcgattggac gccctccggg
     2341 gacaggcaaa gcggggatcg gtgcgcggtg aggaaggagg attctgccat ggcgacgcaa
     2401 ggggatgaat ttcccgcgga agtcggtttt gccgcgattt ttgcggcgtc gccggatggg
     2461 ctgctggtgg tggacggtag cggccggatc cgtctggcca accctgcggt ggagcacatg
     2521 ttcggttacg ccgcgggcga actgatcggc caggccatcg aggtgctggt gccggcgcac
     2581 gccgcgggtc accatgtccg ggaccgcatg gcctacgccg cggcgccgcg gacgcggccg
     2641 atgggtgcat cgaccgagct cgccggacgt tgtcgcgacg gcagcctggt gccggtggac
     2701 atcatgctgg cgccggtgga tgccacgggc acgcggggca ccctctgcgt ggtgcgcgat
     2761 gcccggccgc gccatgccac cgaggccaag ctccacctgg ccagcgcggt gttccagacg
     2821 acccaggagg cgatcgtcgt caccgatacg caatgccgca tccttgctgt caatccggcc
     2881 ttcgagcggg tcaccgaata cgccgaggcg gaggtgctgg ggcagcacat gcgcatcgtg
     2941 gcctcgggcc gccacgaccg atcgttctac gacgccatgt ggacgtccat ccacaccacc
     3001 ggcgaatggc agggcgccat ctggaatcgg cgcaggagcg gggagatcta ccaggagtgg
     3061 ctgtccatca gcacggtgcg cgaccgggaa gggcgcgcct tccagtacgt gggggtgagc
     3121 gccgacatga cgcggatgaa ccacgccgag acgcccatcg agcgccttgc ccacttcgac
     3181 cccctgacgg gtctgcctaa ccgcctgtta ctggactcgc gcatccgcaa gacctgggag
     3241 cgggcgcacc gggacgggct gcgtttcgcc gtgctgttcc tcgatctgga cggcttcaag
     3301 gccgtgaatg atgatcgggg gcatgctgtc ggtgatgagc tgctgcgcgt gattgcagcc
     3361 cgcctggccg gctcggtgcg ggaatcggat acggtggcgc gcctgggtgg cgacgagttc
     3421 atcgtggtgc tggaggatct gcgccgcgag gatgtggcgc ggctggcaga caagctgatc
     3481 gcgcgggtgg ccgagcctgt ggagctgagc gacggctccc aggcccacgt ggggctcagc
     3541 cttggttgga gcacctatcc ggacgacgcc caggacatcg acaccctgat gggtcaggcc
     3601 gatacggcct tgtaccgggc caagcggggc ggtcgcggcc gggcggtcgc cttcgtcggg
     3661 tcggggccgg cctgatcgac ggtctctaag ggcgcgcgct cgcctcggca ggcggtggaa
     3721 gcggcgcggt gtccggaacg ggtaccgcag cggcaggcgc ggccgcaggt ggaggcgcga
     3781 gatcctccgg cggtggcagc tcgggcccca ccggcggggg cggtggcgtt tctgccggcg
     3841 gggcggcgcg cggtgccttg cgcggcggcg caggcggcgc ctcggggcga tcgaacagat
     3901 cccgcagcca gtcccacgcg cgcgtcagca gacccggtcg ggcctgggag acaaagcgcg
     3961 ccttgccatc cacgagtcgt tcggcgacgg cgtcgtggag caggtcgccg acgatgggca
     4021 gggcgctgtg ggcgccctgg ccccagtagt cgctgcgcag ggtgacgcgg ctgtcgttga
     4081 agccgaccca ggcaccggcc accagctgcg gatgcatcgc gatgaaccag ccgtcggtgt
     4141 tgtcctgggt ggtgccggtc ttgcctgcca gatcggcgcg caggccgaag cggctgcgca
     4201 gggcattgcc ggtgccccgg tcgatgacgc cgcgcagggt gtcgagcagc gtataggcga
     4261 cggccgggtc gagggcctct tcgggcgctg gcggggcgat gcgctccagt atgtggccgt
     4321 cggcatcttc cacctgggtg accagcagcg gctcccggta gtggccgccg tcggcgatgg
     4381 cggcgtaggc ggtgaccatc tcccgcaggg tgacagggct ggtgcccagg gccagggacg
     4441 gcacttcctc cagcggactc tggcgcacgc ccagggcgcg ggcgaggtgc gccacccgcg
     4501 ccgggccgac ctgttgcatg agctgggcgg tgatgcggtt gcgggagtac gcgaggccgt
     4561 cccgcaggct gaccgggcgg ccgctgggcg gggattcgtc ggtggggcgc cagacctcgt
     4621 cgccgcgcac ggggatctcc accgccacgt cgtcgagggt gtcctcgggc cgcgcgccgc
     4681 gggcgaaggc ggcgccatac acgaagggct tgaaggtgga gccgggctgg cggcgggcct
     4741 gctgcacgtg gtcgaagggg tcctggacga aatcccggct gccgacccag gcgcggacct
     4801 gaccgtcgcg cgggtcgatg gccagcaggc cggcctggat gcgggtcttg tcggcgcgca
     4861 gggcgttgag gaagacggtg tcgcggagca ggcgggcgag cactgcgtcc ggtgcctggc
     4921 cggcggcgac ggcgttgcgg tagtcgcggc tctcgcgcac cagggcggcg accagctcgg
     4981 ggctgccgga ccagcccttg cgtccgctcc acgcctggtc ggccagtgcc tgcaactggc
     5041 ggccgcgacg cgccaccgcg cgggtcgcgt cgacctgcag gcggctatcg agggtggtgc
     5101 gcaccaccag gccgtcggca tagaggttgt agccgttgcg gtcggcccag gcgatcagcc
     5161 acttgcgcac ctgctgggcg aagtggggcg ccgggccggg ggcttcgacc tggcgttcga
     5221 agtcgatgcg caggggcttc ttccggagcg cgtcgaggcg tgccggatcg agctggccgc
     5281 ggcgcgccat ctgggccagc accacgttgc ggcgggcgac cgcccgctcc gggttctgca
     5341 ccgggttgta ggcgctgttg cccttgagca tgcccaccag ggtggcgctc tcgagtatgt
     5401 cgagctggcg cgcggagtgg tcgaagtagg tgcgcgcggc catctcgatg ccccacgcgt
     5461 tgtagaggaa gggcaccgtg ttgaggtagg tctcgaggat ctcgtccttg ctgtagacgg
     5521 cctcgatctt gagggcggtg atggcttccc gcagcttgcg ggtgagggtg ggcgcgcggc
     5581 cgatctcgtc cgggtagagg ttgcgggcga gctgctgggt gatcgtcgag ccgccctggc
     5641 ggtcgccgga gaaagtatgc agcagcgcgc cgccaaggcg gtagaagtcg atcccgtggt
     5701 gttcgtagaa gcggtggtcc tcggtggcga tgagggcctt caccacgtgg ggcgagatgt
     5761 ccgccaggcc gacccattcc cggttcgccc acttgaacac cgcgatctcg cgaccgtccg
     5821 ccgacagcac ccgtgccgga cgctcctgct tggccttgcg cagatcgccg atgccggggg
     5881 tgaaggggat cagcaccagc acccacagca cgaagagcag cggcagcgcc accagggccc
     5941 gcagtaactc gcgccgggtg ggccggcgca gccgcccgcg cagggcctgg aggcgttcgg
     6001 aaaggccgat cacgcggtca gctcgccggc cgcggcgggc ctcagcccag gaacatccgg
     6061 taggccgggt tcagggtctc gtccacatat tcgtagccga gcttgtcgag gaagccctgg
     6121 aaggcgtccc ggtcggcggg cggcacctgc atgcccacca gcacgcgccc gaagtcggca
     6181 ccgtggttgc ggtagtggaa caggctgatg ttccagtcgg agcgcagggt ttccaggaag
     6241 ttgagcaggg cgccggggcg ctccgggaag atgaagcggt acagcacctc gttggtcgcc
     6301 tgcggcgcat ggccgccaac catgtagcgg atgtggctct tggccatctc gtcgtcggtg
     6361 aggtcgatgc agggcagctc gtggcggtgc agcagttcgg tgaggcgcac gccttcggcc
     6421 cggttgtgca cgcccacgcc gacgaagatg tgggcgcgat gggcgtccgc gtagcggtag
     6481 ttgaactcgg tgatgttgcg gctgcccagc agggtgcaga acttcttgaa ggagccgggc
     6541 ttctcgggga tggtgacggc cagcacggct tcgcgctgct cgcccacctc ggcgcgctcg
     6601 gcgacgaagc gcaggcggtc gaagttcatg ttggcgccgg aggccaccgc caccagggtc
     6661 ttgtccttga gggcgtggcg cttggcgtat tccttggcgc cggctaccgc cagcgcgccg
     6721 gcgggctcga ggatggagcg ggtgtcctcg aagacgtcct tgatggcggc gcagatggcg
     6781 tcgttgtcca ccaggatcat ctcgtccacg taggcctggc acaggcggaa ggtttcctcg
     6841 cccaccagct tgaccgccgc gccgtcggcg aagaggccca cattggccag ttcgatgcgc
     6901 ttgccggcgg ccagggactg ggtcatggcg ttggcgtcca cggtttccac gccgatcacc
     6961 ttgatgtccg gccgcacccg cttgatgtag gccgccacgc cggccagcag gccgccgccg
     7021 ccgacgcagc agaacaccgc gtggatgggc ttgggatgct ggcgcaggat ctccatgccg
     7081 atggtgccct ggccggcgat cacttccacg tcgtcatagg gatggacgaa ggtgagcttc
     7141 tcggccttct cgagctcctt ggcgtgcagg tagcagtcgg agtagctctc gccggccagc
     7201 accacctcgc cgccatggcg ccggaccgcg tcgatcttga tggccggcgt ggtggtgggc
     7261 atgacgatca cggcgcgcac gccgagcttc ctggccgaca gggccacgcc ctgggcgtgg
     7321 ttgccggcgg aggcgcagat cacgccgcgc ttgagggccg ccgggctgag gctggccatc
     7381 ttgttgtagg ccccgcgcag cttgaagctg aacaccggct gcatgtcctc gcgcttgaag
     7441 aagatccggt tgtggatgcg cgccgacaga ttgggcgcca ggtcgagggg cgtctcgatg
     7501 gccgcgtcgt agacctgggc ggtgaggatc ttctggaggt agtcggggtg ctgcgcgctc
     7561 atggcaaacc tgcgtttcgg ctgggggaag cctgggaggg tagcagcggg accggctgcg
     7621 ctgcaacata cggctttttg cttggagatc ccccatgacc ccgatcctgc gcaggggcac
     7681 gaccgcccgc tattccgatt tcaccgtcta cggcggcgtg gtgtattgcg tcgaggtgcc
     7741 gccagcgggc gccgccgacg cgtccgccca gactgccgcg atgctggcca gcctggaacg
     7801 cctgctcgtc gaggcgggca gcgccaagga ccgcctcctg ctggccaccc tgtacctcac
     7861 cgacatggct gactacgacg ccgtgaatgc agtgtgggac gcctgggtgc cgcccggcac
     7921 ggcgccggcg cgggcctgcg tgcaggtgtg ccgcctggcc gacccggcct ggaaggtgga
     7981 ggtggccgta caggcggcaa ggaactgaat cggtgttgaa aacttgcgtg acgtcaagca
     8041 gcgaatcatc gggaaaacgt taaaaagtgc acgaattttt catttgcagc gcataagatg
     8101 ggggcgtgaa ctttttcacg gcggccgcgg catggcaata ggtgggcgac agggcggagg
     8161 ggccctggat tgcatgcgtc gcggcgggca gtcgacttca atcagggagg ttttcatggc
     8221 gcgttcattc gtttcaaagc tcgccggcgc actgctcgcc gccgggctca gtttggccgg
     8281 gtccgcgcac gcggcgctct tcagcaacat gtacgtcttc ggcgacagcc tgtcggatgt
     8341 gggcaacgat ctggcgatca ccggcggtct ggtgcccgac ccggcctact actcgaatgg
     8401 cgccgtgacg gggcgtttcg tgaatggtta caactacgcg gaccgtctgg cctcgcaact
     8461 gggcctgagc cttgcgccgt ccgtgctggg cggcaacgat tacgcctatg gcggtgcccg
     8521 cagcacctat gcgcgtcccg atctggccgt gctgggtgcg ctcagcttcc agcagcaggt
     8581 gaatcagtac cttggcaacg tcggcggcgt ggtcgatccc aacgccctct acgtgctgtg
     8641 gattggcgcc aacgacatgt ccgatggctt cgctcagacc ctcgccaagc tggcggggac
     8701 gggggtgctc gatacgtcct tcatcggcaa tgcggcggcg aatgccctga ccacgctgtt
     8761 cac
//
LOCUS       DAOQPA010000131         8756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4354_length_8756_cov_18.163429, whole genome shotgun sequence.
ACCESSION   DAOQPA010000131 DAOQPA010000000
VERSION     DAOQPA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4354_length_8756_cov_18.163429"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKX01_14610"
     CDS             complement(<1..683)
                     /locus_tag="PKX01_14610"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HMZ77412.1"
                     /translation="MTDLWPRLAACLEHLAPAVPADALAEADTHRDTLAPRLVAVLDG
                     LVADPTPARADGYVLHLYAMLLLACWRDARAYRPLAALGHLDERIIDEVFGQLVHDTY
                     GRALASTCDGDLAPLTALADDPAASQWARAAALEALLVAALEGRAARTAVIAFLADFG
                     AREAQALRDNPADGEHFQLIDVVAVTAADLGAVELLPAIEAWYREGLIDSSFAEAADI
                     AADIARSPD"
     gene            complement(689..3511)
                     /gene="uvrA"
                     /locus_tag="PKX01_14615"
     CDS             complement(689..3511)
                     /gene="uvrA"
                     /locus_tag="PKX01_14615"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ77413.1"
                     /translation="MDAIRIRGARTHNLKNVALDIPRDRLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARAGTPHCPDHPEHALEAQSVSQMVDAVLALPEDTRLMIVAPVISGRK
                     GEQADLFAELRAQGFVRVRVDGAVHELDSLPALDKNRRHSIEVVVDRFKVRQDLRGRI
                     AESFETALTHAEGRAIAVEMDSGTEHLFSARFACPVCSFALAELEPRLFSFNNPAGAC
                     PRCDGLGTVEFFDPARVVAHPELSLAAGAIRGWDRRNQFYFQLLASLASHYEFDIDTP
                     FESLPDALRQVVLHGSGRDKIAFRYLGDGGKAVLKEHAFEGIIPNLERRYRETDSLAV
                     REELAKYRATCACPACGGTRLRTEARHVLVGERNLSEVSRLPLAQCRAFFDTLALSGQ
                     RAQVAEKIVAEIAARLQFLINVGLDYLTLDRSADTLSGGEAQRIRLASQIGSGLTGVM
                     YVLDEPSIGLHQRDNDRLLDTLRRLRDMGNTVIVVEHDEDAIRMADYVVDMGPGAGEH
                     GGRIIAAGRPEDIAAHPESLTGAFLSGTRTIPVPAERRAPEPERLLRIEGASGNNLRD
                     VSLELPVGLFTCVTGVSGSGKSTLINDTLYAHVARHLNGSSVEAAPCTALDGLDHFDK
                     VINVDQSPIGRTPRSNPATYTGLLTPIRELFAGVPDAKARGYGPGRFSFNVKGGRCEA
                     CQGDGLIKVEMHFLPDMYVPCDICHGKRYNRETLEIRYKGKTIYDVLEMTVEQALDFF
                     APVPVVARKLDTLMQVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDE
                     PTTGLHFADIELLLSVLHRLRDHGNTVVVIEHNLDVIKTADWLIDLGPEGGNGGGLIV
                     AAGTPEALAAHPASHTGRYLARLLKA"
     gene            3602..4771
                     /locus_tag="PKX01_14620"
     CDS             3602..4771
                     /locus_tag="PKX01_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ77414.1"
                     /translation="MSPAERRAGVSLAAIFALRMLGLFLVLPVFAVHAHGLPGGDNVG
                     LVGFAIGAYGLTQAFLQIPFGTASDRFGRKPVIVFGLLLFVAGSVVAALAQDVTTIAM
                     GRVLQGAGAISAAVTALAADLTRDQHRTKVMAMIGSSIGLVFALSMVAAPVLYGIIGM
                     SGLFWLTAVLAGGAILTVLFVVPAAPPVPRATGRFAEVLGNGQLMRLNFGVFALHLMQ
                     TAMWVLVPAGLVRAGLPVAEHWKIYLPAVLLSFAVMVPAIIMAERKGAMKPVFNTAIA
                     LLLVVQIGLYVEGSAGLWPLAILLTLFFVAFNVLEATQPSWISKIAPAHAKGTALGVY
                     NTLQSLGLFLGGALGGWLAQHEGPGAVSLLGGGLALVWLALAAGMTPPARRVVPA"
     gene            4839..5360
                     /gene="ssb"
                     /locus_tag="PKX01_14625"
     CDS             4839..5360
                     /gene="ssb"
                     /locus_tag="PKX01_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ77415.1"
                     /translation="MASVNKVILVGNLGKDPETRYAPSGDAICNITVATTDTWRDKAS
                     GEKREATEWHRVAFFGKLAEIAGQYLRKGSQVYIEGSLRTRKWQDKDGQDRYTTEIRA
                     DEMKMLGSRQGMGGGDAPARSEGGYGGGYGGAAPRETAFGGAPAAAPAPSAPPRKPAG
                     GAAFNDFDDDIPF"
     gene            5464..6084
                     /locus_tag="PKX01_14630"
     CDS             5464..6084
                     /locus_tag="PKX01_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ77416.1"
                     /translation="MSANTEVPEKEYCSTTEAAQRLGLSLGTVQQMVENGLLEGWKTA
                     GGHRRIRVSSIEDFRARAAAGGGQTVSRPPGVLQVLVAEDDGILQKLYEHTFSSWGLA
                     VQVRMVASGIDGLLEIGRMPPDLLITDLRMPGIDGFEMIRRLRADPQLADLDIVVVSG
                     LTDEEIAENGGLPADVTLYGKPVPFRELKGYVQAKIMAMQRNRKTA"
     assembly_gap    6206..6305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6531..7088
                     /locus_tag="PKX01_14635"
     CDS             6531..7088
                     /locus_tag="PKX01_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77417.1"
                     /translation="MLEIRREAGALDGTQWHAAGAPSGYMTDPVSDAALTGAAFVRHL
                     GDARFASLRALRQLLRALDAERVAIGVLDGSGTRLHEELVWSSGFGRCGESERDMALS
                     RFADDLAVWQHGTALSLLVDIDVSSEAWQDVPCNQLLLVRGPNHGAGCPVFVAQLRKT
                     LNLPEGAAHAISLLLNEYSQRRVAA"
     gene            complement(7090..7575)
                     /locus_tag="PKX01_14640"
     CDS             complement(7090..7575)
                     /locus_tag="PKX01_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HMZ77418.1"
                     /translation="MGVVILAAGNPSRGDDALGPLLLAELAALAPDGVTLVEDFQFQI
                     EHALDLVGQDIALFIDAAASGAAPFCFAEIRPARSRAPSSHALSPDAVLQVYAETLGV
                     PPPAFVLAVRGASFALGAPLSSVAQAHLEAARPLLAACLAHPDVDAWRRLVHPRPSAA
                     D"
     gene            complement(7581..>8756)
                     /locus_tag="PKX01_14645"
     CDS             complement(7581..>8756)
                     /locus_tag="PKX01_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HMZ77419.1"
                     /translation="ITPTAEKIRRLMHYGQILQSHALHFFHLASPDLLFGFDAPAQQR
                     NIIAVAAAHPDIATHGILLRKFGQEVIRITAGKRIHGTGAIPGGVNKSLSPAERDELL
                     RDVDTMIDWSRDAVALARRLHESDADLYDHFGEVPGQFLSLVRPDGALDLYDGVLRLR
                     GADGRVVRDGIPCTDYLGHITEAVRPWSYMKFPYITTQGPDTGWYKVGPLARVQNCDL
                     IPTPRAEAARQAFLAHGGGAPLHGPLAFHWARMIEMLHAAEVIRELLDDPALLSDDRM
                     SGGPRRREGIGIIEAPRGTLIHHYQVDDDDLVSDCNLIVSTTHNNQAMNTAVRAVARR
                     YLDGQTLTEGLLNHIEVAIRAFDPCLSCATHALGKMPLALELRDMAGVLVDRLQRDT"
BASE COUNT         1253 a         2675 c         3288 g         1440 t
ORIGIN      
        1 tcgtccgggc tgcgggcgat gtcggcggcg atgtcggcgg cctcggcgaa gctgctgtcg
       61 atgaggcctt cccgatacca ggcctcgatg gcgggcagga gttcgacggc gcccaggtcg
      121 gcggcggtca cggcgaccac gtcgatgagc tggaagtgct ccccgtcggc cggattgtcg
      181 cgcagggcct gggcctcgcg ggcgccgaag tcagccagga aggcgatgac ggcggtgcgc
      241 gctgcgcgcc cttcgagggc ggcgacaagc agggcctcca gcgctgcggc gcgggcccac
      301 tggctggcgg cggggtcgtc ggcgagggcg gtgaggggcg ccaggtcgcc gtcgcaggtg
      361 gaggcaaggg cgcggccgta ggtgtcgtgc acgagctggc cgaagacttc gtcgatgatg
      421 cgctcgtcca ggtggccgag ggccgccagg ggccggtagg cgcgggcatc gcgccagcag
      481 gcgaggagca gcatggcgta gaggtgcagc acatagccgt cggcgcgggc gggggtgggg
      541 tcggcgacga ggccgtcgag cacggcgacg aggcggggcg ccagggtgtc ccggtgggtg
      601 tcggcttcgg ccagggcgtc ggccggcacg gcgggcgcga ggtgttcgag gcaggcggcg
      661 agacgcggcc agagatcggt catgggcttc aggccttcag gaggcgggcg aggtagcggc
      721 cggtgtggct ggcgggatgg gcggcgaggg cttccggggt gccggcggcg acgatcaggc
      781 ccccgccgtt gccgccctcg gggcccaggt cgatgagcca gtcggcggtc ttgatcacgt
      841 ccaggttgtg ctcgatgacg accaccgtgt tgccgtggtc gcgcaggcgg tggaggacgc
      901 tgaggagcag ttcgatgtcg gcgaagtgca ggccggtggt gggttcgtcg aggatgtaga
      961 gggtgcggcc ggtgtcgcgc ttggagagtt cgagggccag cttgacgcgc tgggcctcgc
     1021 cgccggagag ggtggtggcg ctctggccca ggcggatgta gccgaggccg acctgcatca
     1081 gggtgtcgag cttgcgggcg accacgggca ccggggcaaa gaaatccagg gcctgctcga
     1141 cggtcatctc cagcacgtcg tagatggtct tgcccttgta gcggatttcc agggtttccc
     1201 ggttgtagcg cttgccgtgg cagatgtcgc aggggacgta catgtccggc aggaagtgca
     1261 tctccacctt gatcaggccg tcgccctggc aggcttcgca gcgtccgccc ttgacgttga
     1321 agctgaagcg gccggggccg tagccgcggg ccttggcgtc gggcacgccg gcgaagagtt
     1381 cgcggatggg cgtgagcagg cccgtgtagg tggccgggtt ggaacgcggt gtgcggccga
     1441 tggggctctg gtcgacgttg atgaccttgt cgaagtggtc gaggccgtca agcgccgtgc
     1501 acggcgcggc ctccacgctg ctgccgttga ggtggcgggc cacgtgggcg tagagggtgt
     1561 cgttgatgag ggtgcttttg ccggagccgg acacgccggt gacgcaggtg aacagcccga
     1621 cgggcaattc gaggctgacg tcgcgcaggt tgttgcccga ggcgccctcg atgcgcagca
     1681 ggcgctcggg ctcgggcgcg cggcgttcgg ccggcacggg gatggtccgc gtgccggaca
     1741 ggaaggcgcc ggtgagggac tccgggtggg cggcgatgtc ctcgggccgg ccggcggcga
     1801 tgatccgccc gccgtgctcg ccggcgccgg ggcccatgtc caccacgtag tcggccatgc
     1861 ggatggcgtc ctcgtcgtgc tccaccacga tcaccgtgtt gcccatgtcg cgcaggcgcc
     1921 gcagggtgtc gaggaggcgg tcgttgtcgc gctggtgcag gccgatggag ggctcgtcga
     1981 ggacgtacat gacgccggtg aggccggagc cgatctggct ggcgaggcgg atgcgctggg
     2041 cttcgccgcc ggacagggtg tcggcggagc ggtccagggt gaggtagtcg agtcccacgt
     2101 tgatgaggaa ctgaaggcgg gcggcgatct cggcgacgat cttctcggcc acctgggcgc
     2161 gctggccgct gagggccagg gtgtcgaaga aggcgcgaca ctgggccagg ggcaggcggc
     2221 tcacctccga caggttgcgc tcgccgacga ggacgtggcg cgcctcggtg cgcaggcggg
     2281 tgccgccgca ggccgggcag gcgcaggtgg cgcggtactt ggcgagctcc tcgcgcacgg
     2341 cgagggagtc ggtctcccgg tagcggcgtt ccaggttggg gatgatgcct tcgaaggcgt
     2401 gttccttgag caccgccttg ccgccgtcgc ccaggtagcg gaaggcaatc ttgtcgcgtc
     2461 ccgagccgtg cagcaccacc tggcggaggg cgtccggcag ggactcgaaa ggcgtgtcga
     2521 tgtcgaattc gtagtggctg gcgaggctgg cgaggagctg gaaatagaac tggttgcggc
     2581 ggtcccagcc gcggatggcg ccggcggcga gggacagctc cgggtgggcg acgacccgcg
     2641 ccgggtcgaa gaactccacg gtgccgaggc cgtcacatct gggacaggcg ccggccgggt
     2701 tgttgaagga gaacaggcgt ggctcgagct cggcgagggc gaagctgcac accggacagg
     2761 cgaagcgcgc cgagaagagg tgctcggtgc cgctgtccat ctccacggcg atggcgcggc
     2821 cctcggcgtg ggtgagggcg gtctcgaagg attcggcgat gcgcccgcgc aggtcctggc
     2881 gcaccttgaa gcggtccacc accacctcga tgctgtggcg gcggttcttg tcgagggcgg
     2941 gcaggctgtc cagctcgtga acggcgccgt ccacgcggac gcgcacgaag ccctgggcgc
     3001 gcagctcggc gaagaggtcg gcctgttcgc ccttgcggcc ggagatgacc ggcgcgacga
     3061 tcatcagccg ggtgtcttcc ggcagggcca gcacggcgtc caccatctgg gacacgctct
     3121 gggcctccag ggcgtgctcg gggtggtccg ggcagtgggg cgtaccggcg cgggcgtaga
     3181 gcaggcgcag gtagtcgtgg atctcggtga cggtgccgac ggtggagcgg gggttgtggc
     3241 tggtggcctt ctgctcgatg gagatggccg gggagaggcc ctcgatgagg tccacgtcgg
     3301 gcttttccat cagctgcagg aactgccggg cgtaggcgga gagggattcc acgtagcgcc
     3361 gctgcccctc ggcgtagagg gtgtcgaaag ccagggagct cttgcccgag cccgacaggc
     3421 cggtgatcac ggtgagccgg tcgcggggga tgtcgagggc gacattcttg aggttgtggg
     3481 tgcgggcacc gcggattcgg atggcgtcca tggggcattc ggggtggggg caacctgtta
     3541 atatacgcct cttccccctc ccgacgcagc acgattcatg gcttcccaga acgacgacaa
     3601 gatgagtccg gccgagcgcc gtgcaggcgt ttcgctggcg gcgatctttg cgctgcgcat
     3661 gctgggcctg ttcctggtcc tgccggtgtt cgcggtgcat gcccacggtc tgcccggcgg
     3721 cgacaacgtg ggcctggtgg gctttgccat cggcgcctat ggcctgaccc aggctttcct
     3781 gcagattccc ttcgggaccg cgtcggaccg cttcggacgc aagccggtga tcgtcttcgg
     3841 cctgctgctc ttcgtcgccg gcagcgtggt ggcggcgctt gcccaggatg tgacgacgat
     3901 tgccatgggc cgcgtgctgc agggcgccgg cgccatttct gcggcggtca ccgcgctggc
     3961 cgccgacctc acccgcgacc agcaccgcac caaggtgatg gccatgatcg gctcgtccat
     4021 cggcctggtg tttgccctct ccatggtggc ggcgccggtg ctttacggga tcatcggcat
     4081 gagcgggctg ttctggctga ctgccgtgct ggcggggggc gcgatcctca cggtgctgtt
     4141 cgtggtgcct gccgcgccgc cggtgccgcg ggccaccggg cgttttgccg aggtgctcgg
     4201 caatggccag ctcatgcgcc tcaacttcgg tgtgtttgcc ctgcacctca tgcagacggc
     4261 catgtgggtg ctggtgccgg cgggactggt gcgcgccggc ctgcccgtgg cggaacactg
     4321 gaagatctat ctgccggccg tgctgctgtc ctttgcggtc atggtaccgg ccatcatcat
     4381 ggcggagcgc aaaggggcca tgaagccggt cttcaacacc gccatcgctt tgctgctggt
     4441 ggtgcagatc gggctttatg tggaaggttc ggcgggcttg tggccgctgg ccatcctgct
     4501 gaccctgttc ttcgtcgcct tcaatgtcct cgaggccacc cagccgtcgt ggatttccaa
     4561 gatcgccccg gcccacgcca aggggacggc gctcggggtg tacaacacgt tgcagtccct
     4621 cgggctgttc cttggcggag cgctcggtgg ctggctggcg cagcacgagg ggcccggtgc
     4681 ggtgtccctg ctgggcggcg gcctggctct ggtgtggctg gcgctcgccg ccggcatgac
     4741 gccgccggcc cggcgcgtcg tcccggcctg atccgccccg cccccctgcc cgccacccat
     4801 cggcgcggct ccatcggact tacattcagg agagaactat ggcatcggtc aacaaggtga
     4861 tcctcgtcgg caatctgggc aaggatccgg aaacgcgcta cgcccccagc ggcgatgcga
     4921 tctgcaacat caccgtcgcc accaccgaca cgtggcgcga caaggccagc ggcgagaagc
     4981 gcgaagcgac cgagtggcac cgggtggcct tcttcggcaa gctcgccgaa atcgccggcc
     5041 agtatctgcg caagggcagc caggtgtaca tcgagggctc cctgcgtacc cgcaagtggc
     5101 aggacaagga tggccaggac cgttacacca ccgagatccg ggccgacgag atgaagatgc
     5161 tgggcagccg gcagggcatg gggggcggcg acgccccggc tcgcagcgag gggggatatg
     5221 gcggtggata cggcggcgcc gcaccgcggg agacggcctt cggcggcgct ccggccgcgg
     5281 ctccggcccc gtccgcaccg ccccgcaagc ctgcgggcgg cgcggcattc aatgattttg
     5341 atgacgatat cccattctga tactctactg cctattcctt tagttgtagg tgtatgggta
     5401 ttccatacgt ttcgttactg ttaagatcat gcctgttcac gttgatcgcc tgctgccctg
     5461 accatgtctg ccaacactga agttccggaa aaggaatact gcagcacgac cgaagccgcc
     5521 cagcggctgg gtctttcgct gggtaccgtc cagcagatgg tggaaaacgg cctgctggaa
     5581 gggtggaaga ccgctggcgg gcatcgtcgg atccgggtca gttccatcga ggacttccgg
     5641 gcccgcgcgg cggccggtgg cggtcagacc gtgtcccggc cgcccggtgt cctgcaggtg
     5701 ctggtcgccg aggacgatgg catcttgcag aagctctacg agcacacgtt ctcgtcctgg
     5761 gggctggccg tccaggtgcg catggtggcg agcggcatcg acggcctgct ggaaatcggc
     5821 cgcatgccgc cggatctgct catcaccgac ctgcgcatgc cgggcatcga cggcttcgag
     5881 atgatccgcc gcctgcgggc tgatccgcag ctggccgatc tggacatcgt cgtggtgagc
     5941 ggcctgaccg acgaggaaat cgccgagaac ggcggcctgc cggcagacgt gaccttgtat
     6001 ggcaagcctg tccccttccg cgagctgaag ggctatgtgc aggccaagat catggccatg
     6061 cagcgcaatc gcaagacggc ctgaccaccc tgcgccgtcg tcggcgcggt tgcccctgaa
     6121 agccctccgc tatccggagg gctttttcat ggtgccgtca caacgtgatg ccgcccgatg
     6181 cgaggtacgg agtctggcgg ccggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnngcagc ctccgccgcg gacggcatga tttgaatgtt tgtgacaaat tcgatgtttt
     6361 tgtgatttgt ggcagaatgt ctgcggaatt gtgcacgaat gccggacgga tgaatccgta
     6421 cactttcccg tcatcggatg gcgccgcgaa acgggaaaaa aagcccgcga ccggaccctc
     6481 cttccggaat caggctccgc ccggcggagc cgcattgagg agcagacgcg atgcttgaga
     6541 tcagacgtga agcaggggcc ctggacggca cccagtggca cgcggcgggt gcaccgtcgg
     6601 gctacatgac ggacccggtt tccgacgccg ccctgaccgg cgccgccttc gtgcgccacc
     6661 tgggcgatgc gcgctttgcg agcctgcggg ccctgcgtca gctgctccgt gccctggacg
     6721 cggagcgggt ggcgatcggc gtcctcgacg gaagtggcac gcgcttgcac gaggaactcg
     6781 tgtggagttc cggtttcgga cgttgcgggg agtcggagcg ggacatggcc ctgtcacgct
     6841 tcgccgacga tctggcggtg tggcagcacg ggacggcctt gtccctgctg gtggatatcg
     6901 acgtgtcgtc cgaggcctgg caggacgtcc cctgcaacca gctcctgctg gtccgcggtc
     6961 cgaaccatgg cgcggggtgt ccggtgtttg tggcgcagct gcggaaaacc ctgaatctgc
     7021 cggaaggcgc cgcccacgcc atttccctgc tgctcaacga atactcccag cgtcgcgtcg
     7081 cggcctgatt cagtccgctg cggacgggcg cggatgcacg aggcgccgcc acgcgtccac
     7141 gtccgggtgg gccagacagg ccgccagaag cggtcgcgcc gcctccagat gagcctgggc
     7201 cacagacgac aggggcgccc ccagtgcgaa ggatgcgccc cgcacggcca gtacgaaagc
     7261 cggtggcggc acgccgaggg tctccgcata cacctgcaac accgcgtcgg ggctcagggc
     7321 gtggctgctc ggtgcgcggc tgcgggccgg ccggatctct gcaaagcaga agggggctgc
     7381 gccgcttgcc gcggcatcga tgaagagggc aatgtcctgc ccgaccagat ccagcgcgtg
     7441 ttcgatctgg aactggaaat cctccaccag cgtgaccccg tccggcgcca gggcggccag
     7501 ctcggccagc agcagaggcc cgagggcatc gtcgcctcgc gagggattgc cggcggcgag
     7561 gatcaccacg cccatgccgt tcaggtgtcc cgttgcaggc gatccaccag gacgccggcc
     7621 atgtcccgca gttccagcgc caggggcatc ttgcccagtg cgtgggtggc gcaggacaga
     7681 caggggtcga aggcgcggat cgccacctcg atgtggttga gcaggccctc ggtcagtgtc
     7741 tgtccgtcca ggtagcggcg ggcgacggcg cgcaccgcgg tgttcatcgc ctggttgttg
     7801 tgggtggtgg acacgatcag gttgcagtcg ctgaccagat cgtcgtcgtc cacctggtaa
     7861 tggtggatca gcgtgccgcg gggggcttcg atgatgccga tgccctcgcg ccgacgcggg
     7921 ccgccagaca tccggtcgtc agagagcagg gccggatcgt cgaggagctc gcggatcacc
     7981 tcggcggcgt ggagcatctc gatcatgcgc gcccagtgga aagccagcgg accatgcagc
     8041 ggggcgccgc cgccgtgggc gaggaaggcc tggcgcgccg cctcggcgcg gggcgtgggg
     8101 atgaggtcgc agttctgcac ccgcgccagg gggccgacct tgtaccagcc ggtgtccggc
     8161 ccctgcgtgg tgatgtaggg gaacttcatg taactccagg gcctgaccgc ctcggtgatg
     8221 tggccaaggt aatcggtgca ggggatgccg tcccgcacca cacggccgtc ggccccgcgc
     8281 agacgcagga cgccgtcgta caggtcgagg gcgccatcgg ggcgcaccag gctgaggaac
     8341 tgcccgggca cttcgccgaa atggtcgtag agatccgcat cgctctcgtg caggcggcgg
     8401 gcgagggcca cggcgtcgcg gctccagtcg atcatggtgt ccacgtcgcg cagcagttcg
     8461 tcgcgctcgg cggggctgag ggacttgttg acaccgccgg ggatcgcgcc ggtgccgtgg
     8521 atgcgtttgc ctgcggtgat gcggatcact tcctgaccga acttgcgcag caggatgcca
     8581 tgggtggcga tgtccggatg ggcggcggcg acggcgatga tgttgcgctg ctgggccggc
     8641 gcatcgaagc cgaacagcag gtccggcgag gccagatgga agaagtgcag ggcgtgggac
     8701 tggaggatct ggccgtagtg catcaggcgg cggatcttct cggcggtggg cgtgat
//
LOCUS       DAOQPA010000132         8731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4375_length_8731_cov_16.491240, whole genome shotgun sequence.
ACCESSION   DAOQPA010000132 DAOQPA010000000
VERSION     DAOQPA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4375_length_8731_cov_16.491240"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKX01_14650"
     CDS             <1..412
                     /locus_tag="PKX01_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77420.1"
                     /translation="LANVGAGVVVGKLGTATVSVDELAAALHAPAGGARGIVDEPRLA
                     AAVAQARDAGERVVLYPAAFDGLRPADLRALQAARRQGDRLVAAVLNDAAATCALGER
                     LALAAALEAVDWTIACDESPAELAARIGAELAVS"
     gene            423..1370
                     /gene="rfaD"
                     /locus_tag="PKX01_14655"
     CDS             423..1370
                     /gene="rfaD"
                     /locus_tag="PKX01_14655"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HMZ77421.1"
                     /translation="MIIVTGGAGFIGSNIVQGLNARGVTDILVVDDLSDGRKCLNLSD
                     AHIHDYLDKDDFLARLQAGESFGPVEAVFHQGACSATTEWDGRFVMNVNYAYSKAVLR
                     WCVERKVPFYYASSASVYGEGPVFKEDRAHEHPLNAYAYSKFQFDCHLRPQLAGFRSP
                     VVGLRYFNVYGPREQHKGSMASVAYHFNTQLKEGDRLRLFEGSDGYGPGEQRRDFIHV
                     DDVVAVNLWLLEHPEVSGIYNCGTGRAQPFNDVARATIAFHGRGHIEYIPFPEHLKGR
                     YQSYTQADMGALRAAGYDRPFLSVEEGVARYMAWLAKRS"
     gene            1383..2414
                     /gene="waaF"
                     /locus_tag="PKX01_14660"
     CDS             1383..2414
                     /gene="waaF"
                     /locus_tag="PKX01_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HMZ77422.1"
                     /translation="MAPRRILVVGPSWVGDMVMAQSLFMTLKARHTLVEIDVLAPGWS
                     LPILARMPEVRRGIAMPLGHGQFGLRTRWQLGRQLAQDRYDQAIVLPGSFKSALAPFF
                     AGIPRRTGFRGEMRFGLLNDMRRLDKRALPMTVQRFVALGLPPDTPQPPPTPTPRLQP
                     DPAGFARLRAQLDLLADRPGVCFMPGAEYGPAKQWPIPSYGELGRRLVRQGFQVWVLG
                     SAKDKPAGDEIATLAGEGAFNLCGRTELADAVDLLATARAAVTNDSGLMHVAAALGTP
                     LVALYGSSTPHHTPPLSDAAQVIYLGLECSPCFKRTCPLGHTRCLTGIGVERVAEALA
                     PALHATAPR"
     gene            2411..3646
                     /locus_tag="PKX01_14665"
     CDS             2411..3646
                     /locus_tag="PKX01_14665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ77423.1"
                     /translation="MNTRPPFQDRAADAARWLCVASAFAVPLPAAWLSFTTGLFLIAW
                     GLAGRLPLRWATIRQHPVGRLSLVLLLWMAVAIAWSPVEPKVGLAHWWHYRELLIVPL
                     MLSLFDADTARRWQPRVFTAFFIGFGIALVTSFLRWTGVLPDMGFKGLYAGFGGRTGF
                     SMLLAWAIFCAVEFGRDQPRFRLAWAALALVCFANLFFINTGRTGQIAFFTLVPLSLY
                     HLAGRRGLVAGLLAIPVLAAGMYAASPAVRARIAESAEHIAAYEAGHPYTGDGLRLEF
                     IANAADFIRQHPVFGGGTGSFAAEYKVVAEREGMTGERVADNPHNEYLMVWSQQGLPG
                     LALLIALFLTQWRLSARLDDLPARLNRGLVLLIAGGDLVNSLILDNLEGHLWALLTVA
                     LTAGIGADRAAPSPTESSR"
     gene            3648..4394
                     /locus_tag="PKX01_14670"
     CDS             3648..4394
                     /locus_tag="PKX01_14670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ77424.1"
                     /translation="MARPTLSVIIIARNEAALIAKCLDSVTWADEIVVVDSGSTDGTP
                     EICRRYTDKVFVTPDWPGFGPQKNRALDLATSDWVLALDADETISIPLATEIAATIAL
                     TPHDVYAVKRLSSFLGRPIRHGDWWPDEVPRLFRRGCARFSDDPVHERLVFEGRPARL
                     RALMMHDSIRSLDQMCAKMNHYTSAGAERLARAGKRGGLGKAIRHGLWAFLRAYFFKR
                     GFLDGREGFILAVSAAESAYYRYLKRLYLP"
     gene            4391..5380
                     /locus_tag="PKX01_14675"
     CDS             4391..5380
                     /locus_tag="PKX01_14675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ77425.1"
                     /translation="MSASADLAARRFSVVIVNYNGGQMLLDCVRSVRSGLLPADRIIV
                     VDNGSRDDSMARLEAAEPGCVILRNGCNAGFARAVNRGLAQVRSEFALLLNNDARLAP
                     DAWDALAAAFDTLPRAAFLGGRLLYEDGRLQNAVAAFPRLSAELLPRALLRALWPARY
                     RGRPTGDAPLRVESIIGALFAVRMAAVAEFGPLDEDFFFFLEETEWCHRAHTRGWETW
                     HIPAARAVHAQGATAKKHNALARIEYHRSRLLYFRKTAPGRYALSYAIALTKAAFNAL
                     GNALLAGLTLGLAPRVRAKAGVDLKILAWYLQGRPADVGLPDKCPPGRPLPGA"
     gene            5387..6361
                     /gene="waaC"
                     /locus_tag="PKX01_14680"
     CDS             5387..6361
                     /gene="waaC"
                     /locus_tag="PKX01_14680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HMZ77426.1"
                     /translation="MKLLVVKTSSLGDVIHMLPAVTDAARARPGLTVDWLVEESFAAI
                     PPWHPAVRRVLPIAMRRWKKQWRRATTWAQLRAARAALQATDYDWVVDTQGLLKSALW
                     TRWARGPRCGYDAASAREPLAARFYERHYAVPREMHAIERNRRLLAQALGYTLDDPRP
                     DYGLAGLAARLPAPDVSLPARYVVGLHGTSRADKEWPVEHWKALARALGGAGAPLVMP
                     WGNEAERQRAEAIAAAAPGALVLPRLGLDALAVILDRAAAVTGVDTGLMHLAAGLGKP
                     GLALYTATRPALTGVVPDRHSRSALENRERPDALGADAVIAGMLARLA"
     gene            6371..7096
                     /locus_tag="PKX01_14685"
     CDS             6371..7096
                     /locus_tag="PKX01_14685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ77427.1"
                     /translation="MSAILPILMYHQIDATPPRGTPLRGLTVTPGSFARQMALLRLLG
                     YRGVSMHDLEPYLRGERRGRVVGITFDDGYRNNLEHALPVLQKHGFSATCYAVSDPFE
                     GRNAWDEGMGMPQKPLFTPDDMRAWVAGGMELGAHTRHHADLTRIDDATAEDEIAGCK
                     RELEAIIGREVRHFCYPYGHYGPAHRDMVRAAGYRSATTVERSRARSSDDPFELPRVM
                     IARTNHLALFWMKIATGYEDSRG"
     gene            7093..8241
                     /locus_tag="PKX01_14690"
     CDS             7093..8241
                     /locus_tag="PKX01_14690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ77428.1"
                     /translation="MTRRLRIAVLNRHFGRRFGGAESYSVALVEALAARHELHVFAQE
                     FDHPGIGIALHRVPRPLAKPRWLNQLWYAAWTWQATRRGFDVVHSHENTWHGQIQTVH
                     VRPLRVGLFHGLSAGRRILKTLQILTSPRLLAYWWLEGSRFRPRPDRRIVASSTSVRD
                     EVLQAYPHAAGMMSVIPPGTLLPAGPADPAAARRQLKLPADVPLALFAGNDYVKKGLP
                     ALLEALTRLPADLHLAVAGNPAPIAAFRAQAKQLGVGARVHFLGSLRDMVPAYTAADL
                     LVHPTTEDTYAMVVLEALAHGLPVIVSGPAHCGIATELRDGVDALLVDDPRDADALAR
                     AIARVRTDVGLAAALGLAGRAFAAARDWDARAAVQEALYFEAAGNATG"
     gene            complement(8414..>8731)
                     /gene="gatB"
                     /locus_tag="PKX01_14695"
     CDS             complement(8414..>8731)
                     /gene="gatB"
                     /locus_tag="PKX01_14695"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HMZ77429.1"
                     /translation="AGLVQRIADNTISNAIGKKVFEALWNGEGPSADAIIDAQGLKQV
                     TDSSAIEALIDEVLAANPKSVDEYRAGKEKAFNALVGQCMKASKGKANPTQVNALLKQ
                     KLG"
BASE COUNT         1186 a         3532 c         2732 g         1281 t
ORIGIN      
        1 cctggccaac gtgggcgccg gggtcgtggt gggcaagctg ggcaccgcca ccgtgtcggt
       61 ggacgagctg gccgccgccc tgcacgcgcc cgccggcggc gctcgcggta tcgtggacga
      121 accccgcctg gccgccgccg tggcccaggc ccgcgacgct ggcgagcgcg tcgtgctcta
      181 tcccgctgcc ttcgacggcc tgcgcccggc cgatctgcgc gccctgcagg cggcccgccg
      241 ccagggcgac cggctggtcg ccgcggtgct caacgacgca gccgccacct gcgcgctggg
      301 cgaacgcctg gccctcgccg ccgcgctgga agcagtggac tggaccatcg cctgcgacga
      361 atcccccgcc gagctggcgg cccgcatcgg ggccgagctc gccgtatcct gaaggagcga
      421 ccatgatcat cgtgaccggc ggcgccggct tcatcggcag caacatcgtc cagggcctca
      481 atgcccgggg cgtcaccgac atcctggtgg tggacgacct cagcgacggg cgcaagtgcc
      541 tcaacctgtc cgacgcccac atccacgact acctggacaa ggacgacttc ctggcccgcc
      601 tccaggccgg cgaatccttc ggcccggtgg aggcggtctt ccatcagggc gcctgctccg
      661 ccaccaccga gtgggacggg cgctttgtga tgaacgtgaa ctacgcctac agcaaggccg
      721 tgctgcgctg gtgcgtcgag cgcaaggtgc cgttctacta tgcctcgtcc gcgtccgtgt
      781 acggcgaagg cccggtcttc aaggaggacc gcgcccatga acaccccctc aacgcctacg
      841 cctactccaa gttccagttc gactgccacc tgcgcccgca actggccggc ttccgcagcc
      901 ccgtcgtggg cctgcgctac ttcaacgtct atggcccgcg cgagcagcac aagggcagca
      961 tggccagcgt cgcctaccac ttcaacaccc agctcaagga aggcgaccgc ctgcgcctct
     1021 tcgagggcag cgacggctac ggccccggcg agcagcgccg cgacttcatc cacgtggacg
     1081 acgtggtcgc cgtcaacctg tggctgctcg agcaccccga ggtctccggc atctacaact
     1141 gcggcaccgg ccgcgcccag cccttcaacg acgtggcgcg ggccaccatc gccttccacg
     1201 gccgcggaca catcgagtac atccccttcc ccgagcacct caagggccgc taccagagct
     1261 acacccaggc cgacatgggc gccctgcgcg ccgccggcta cgaccgcccc ttcctcagcg
     1321 tcgaggaagg cgtggcccgc tacatggcct ggctggccaa acggagctga ccgaccgtga
     1381 gcatggcgcc ccgccgcatc ctggtggtcg gcccctcgtg ggtcggggac atggtcatgg
     1441 cccagagcct gttcatgacc ctcaaggccc gccacaccct ggtggagatc gacgtcctcg
     1501 cccccggctg gtccctgccc atcctggccc gcatgccgga ggtccgccgc ggcatcgcga
     1561 tgcccctcgg ccacggccag ttcggtctgc gcacccgctg gcagcttggc cgccagctcg
     1621 cccaggaccg ctacgaccag gccatcgtcc tgcccggctc cttcaagtcc gccctcgccc
     1681 ccttcttcgc cggcatcccg cggcgcaccg gcttccgcgg cgagatgcgc ttcggcctcc
     1741 tcaatgacat gcgccgcctc gacaagcgtg ccctccccat gacggtgcag cgcttcgtcg
     1801 ccctcggcct gccaccggac acgccccagc cgccgcccac ccccacaccg cgcctgcagc
     1861 ccgatcctgc cggctttgcg cgcctgcgcg cccagctcga cctgctggcc gatcgcccgg
     1921 gcgtatgctt catgcccggc gccgaatatg gccccgccaa gcagtggccc attccatcct
     1981 acggcgaact gggccgccgc ctggtgcggc agggcttcca ggtgtgggtg ctgggctcgg
     2041 ccaaggacaa acccgccggc gacgagatcg ccaccctggc cggcgagggc gccttcaacc
     2101 tgtgcggacg caccgagctc gccgacgcgg tggacctgct cgccaccgcc cgcgccgccg
     2161 tcaccaacga ttccggcctg atgcacgtgg ccgccgccct cggaacgccg ctggtggccc
     2221 tctacggatc ctccacgccc caccacacgc cgcccctgtc ggacgcggcc caggtgatct
     2281 acctcggcct ggaatgctcc ccgtgcttca agcgcacctg ccccctcggc cacacccgct
     2341 gcctcaccgg catcggcgtg gaacgggtgg cggaggccct ggccccggcc ctgcacgcca
     2401 ccgcgccccg atgaacaccc gcccgccttt ccaggatcgc gccgccgacg ccgcccgctg
     2461 gctgtgcgtg gccagcgcct tcgccgtgcc cctgccggcc gcctggctca gcttcaccac
     2521 cggcctgttc ctcatcgcct ggggcctggc cggccgcctg ccgctgcgct gggcaacgat
     2581 ccgccagcac ccggtcggac gcctcagcct ggtcctgctg ctgtggatgg ccgtggccat
     2641 cgcctggtct ccggtcgagc ccaaggtcgg cctggcccac tggtggcatt accgggaact
     2701 cctcatcgtc ccgctgatgc tgtccctctt cgacgccgac accgcccggc gctggcagcc
     2761 gcgcgtgttc acagccttct tcatcggctt tggcatcgcc ctcgtcacct ccttcctgcg
     2821 ctggaccggc gtgctgcccg acatgggctt caagggcctc tatgccggct tcggcgggcg
     2881 caccggcttc agcatgctgc tggcctgggc gatcttctgc gccgtcgagt ttggccgcga
     2941 ccagccccgc ttccgcctcg cctgggctgc gctggccctg gtgtgcttcg ccaacctgtt
     3001 cttcatcaac accgggcgga ccgggcagat cgccttcttc accctggtgc ccctgagcct
     3061 ctaccacctg gccggccgcc gcgggctcgt cgccggcctg ctcgccatcc cggtactggc
     3121 cgccggcatg tacgccgcct cgcccgccgt gcgggcacgc atcgccgaat ccgccgagca
     3181 catcgccgcc tacgaagccg gccatccgta caccggcgac ggcctgcgcc tggaattcat
     3241 cgccaacgcg gccgatttca tccgtcagca cccggtgttc ggcggcggta ccggcagctt
     3301 cgccgccgaa tacaaggtcg tcgccgagcg cgaagggatg accggcgagc gcgtcgctga
     3361 caacccccac aacgaatacc tgatggtctg gtcccagcag ggtctgcccg gtctggccct
     3421 gctgatcgcc ctgttcctca cccagtggcg gctctccgcc cggctggacg acctgccggc
     3481 ccgtctcaac cgcggcctgg tgctgctcat cgccggcggc gacctggtca actccctgat
     3541 cctcgacaac ctggaaggcc acctgtgggc cctgctcacc gtcgccctga cggccggcat
     3601 cggcgccgac cgcgcggccc cttcccccac cgaaagctcg cgctgacatg gcccgcccca
     3661 ccctgtccgt catcatcatc gcccgcaacg aagcagcgct gatcgccaag tgcctcgact
     3721 ccgtcacctg ggcggacgag atcgtcgtgg ttgattccgg cagcaccgac ggcacgccgg
     3781 agatctgccg acgctacacg gacaaagtct tcgtcacccc cgactggccc ggtttcggcc
     3841 cccagaagaa tcgcgccctc gacctcgcca ccagcgactg ggtgctggcc ctggacgccg
     3901 acgagacgat ctccatcccc ctcgccaccg agatcgccgc caccatcgcc ctcacgcccc
     3961 acgacgtgta cgcggtcaag cgcctgtcca gcttcctcgg ccgcccgatc cgtcacggcg
     4021 actggtggcc cgacgaggtg ccgcgcctgt tccgccgggg ctgcgcccgc ttctccgacg
     4081 atccggtaca tgagcgcctg gtcttcgaag gccgtccggc gcgcctcagg gcgctgatga
     4141 tgcacgacag catccgcagc ctcgaccaga tgtgcgccaa gatgaaccac tacaccagcg
     4201 ccggcgccga gcgcctcgcc cgcgccggca aacggggcgg cctcggcaag gcgatccgcc
     4261 acggcctgtg ggccttcctg cgcgcctact tcttcaaacg cggcttcctc gacggccgcg
     4321 aaggcttcat cctcgccgtc tccgccgccg aaagcgccta ctaccgctac ctcaagcgcc
     4381 tgtacctgcc atgagcgcct ccgccgatct cgccgcccgg cgcttctccg tcgtcatcgt
     4441 caattacaac ggcggccaga tgctcctcga ctgcgtccgc tcggtgcgca gcggcctgct
     4501 cccggccgac cggatcatcg tggtggacaa cggctcccgc gacgactcga tggcccgcct
     4561 cgaagccgcc gagccgggct gcgtcattct gcgcaacggc tgcaacgccg gcttcgcgcg
     4621 ggccgtcaac cgcggcctgg cccaggtgcg cagcgagttc gccctgctcc tcaacaacga
     4681 cgcccgcctc gcccccgacg cctgggacgc cctcgccgcc gccttcgaca ccctgccccg
     4741 cgccgccttc ctcggcggcc gcctgctcta cgaggacggc cgcctgcaga acgccgtcgc
     4801 cgcctttccg cgcctgtccg cagagctcct gccgcgggcc ctgctgcgcg ccctgtggcc
     4861 cgcccgctac cggggccgcc ccaccggcga cgcgccgctg cgggtcgaga gcatcatcgg
     4921 cgccctgttc gccgtgcgca tggccgccgt cgccgaattc ggccccctgg acgaggattt
     4981 cttcttcttc ctcgaagaaa ccgagtggtg ccatcgcgcc cacacccgcg gctgggagac
     5041 ctggcacatc cccgccgccc gcgcggtcca cgcccagggc gccaccgcca agaagcacaa
     5101 cgccttggcg cgcatcgaat accaccgctc gcgcctgctc tacttccgca agaccgcgcc
     5161 gggccgctac gccctcagct acgccatcgc gctgaccaag gccgccttca acgccctcgg
     5221 caacgccctg ctcgcggggc tcaccctcgg cctcgcaccc cgcgtgcgcg ccaaggccgg
     5281 cgtggacctg aagatcctcg cctggtatct gcaagggcga ccggccgacg tgggcctgcc
     5341 ggacaagtgc ccgccgggcc ggcccctgcc cggagcctga accgccatga agctgctggt
     5401 cgtcaagacg tcctccctcg gcgacgtgat ccacatgctc cccgccgtca ccgacgccgc
     5461 ccgcgcccgc ccgggcctca cggtggactg gctggtggag gagagcttcg ccgccatccc
     5521 gccctggcac ccggccgtac gccgtgtcct ccccatcgcc atgcggcgct ggaaaaagca
     5581 gtggcgccgc gcgaccacct gggcccagct gcgcgcggcc cgcgccgccc tgcaggccac
     5641 cgattacgac tgggtggtgg acacccaggg cctcctcaag agcgccctgt ggacgcgttg
     5701 ggcccgtggc ccgcgctgcg gctatgacgc cgcctccgcg cgggaacccc tcgcggcgcg
     5761 cttttacgag cgccactacg ccgtgccgcg ggagatgcac gccatcgaac gcaaccgccg
     5821 cctcctcgcc caggccctcg gctacaccct cgacgacccg cgccccgact acggcctggc
     5881 cggcctcgcc gcacgcctgc cggcaccaga cgtgagcctg ccggcgcgct acgtggtcgg
     5941 cctgcacggc accagccgcg ccgacaagga gtggcctgtc gaacactgga aggccctcgc
     6001 ccgcgccctc ggcggcgccg gcgccccgct ggtgatgccc tggggcaacg aggccgagcg
     6061 ccagcgtgcc gaagccatcg ccgctgccgc gccgggcgcg ctggtgctgc cgcgcctggg
     6121 cctcgacgcc ctggcggtca tcctcgaccg ggccgccgcc gtcaccggcg tggacaccgg
     6181 cctgatgcac ctggccgccg gcctcggcaa gcccggcctg gccctgtata ccgccacccg
     6241 cccggcccta accggcgtcg tgccggaccg ccacagccgc agcgccctgg aaaaccggga
     6301 gcgcccggac gcgctgggtg ccgacgccgt catcgccggc atgctcgccc gccttgcctg
     6361 aggaccgccc atgagcgcca tcctgcccat cctgatgtac caccagatcg acgccacgcc
     6421 gccgcggggc acgcccctgc gcgggctcac cgtcaccccc ggcagcttcg cccggcagat
     6481 ggccctgctg cgcctgctgg gctaccgcgg cgtgagcatg cacgacctgg aaccctatct
     6541 gcgcggcgaa cggcgcggtc gcgtggtggg tatcaccttc gacgacggct accgcaacaa
     6601 cctggagcac gccctgccgg tgctgcagaa gcacggcttc agcgccacct gctacgccgt
     6661 cagcgacccc ttcgagggcc gcaacgcctg ggacgaaggc atgggcatgc cccagaagcc
     6721 cctgtttacg ccggacgaca tgcgcgcctg ggtggccggc ggcatggagc tgggcgccca
     6781 tacccgccac cacgccgacc tcacccggat cgacgacgcc accgccgagg acgagatcgc
     6841 cggctgcaag cgcgagctgg aagccatcat cggccgcgaa gtgcgccact tctgctatcc
     6901 ctacggccac tacggccccg cccaccggga catggtgcgc gccgccggct accgcagcgc
     6961 caccaccgtg gaacgcagcc gtgcccgcag cagcgacgac cccttcgagc tgccgcgggt
     7021 catgatcgcc cgcaccaatc acctcgccct gttctggatg aagatcgcca ccggctacga
     7081 ggacagccgc ggatgacgcg ccgcctgcgc atcgccgtcc tcaaccgtca cttcggacgt
     7141 cgcttcggcg gcgccgaaag ctattccgtg gccctcgtcg aagccctcgc cgcccgccac
     7201 gaactgcacg tcttcgccca ggagttcgac caccccggca tcggcatcgc cctgcaccgg
     7261 gtgccgcggc cgctggccaa gccgcgctgg ctcaaccagc tgtggtacgc ggcatggacc
     7321 tggcaggcca cgcggcgcgg cttcgacgtg gtccattccc acgagaacac ctggcacggg
     7381 cagatccaga ccgtgcacgt acgcccgctg cgggtcggcc tgttccacgg gctttccgcc
     7441 gggcggcgca tcctcaagac cctgcagatc ctcaccagcc cgcggctgct ggcctactgg
     7501 tggctggaag gcagccgctt ccggccgcgg ccggaccggc gcatcgtcgc cagctccacc
     7561 agcgtgcgcg acgaagtcct gcaggcctat ccccacgccg ccgggatgat gtcggtgatc
     7621 ccgccgggca ccctgctgcc cgccggcccg gcggacccgg cggcggcgcg acgccagttg
     7681 aaactgccgg ccgacgtgcc cctggccctc tttgccggca acgactacgt caagaagggc
     7741 ctgcccgccc tcctggaggc cctcacccgc ctgcccgccg acctgcacct ggcggtggcc
     7801 ggcaacccgg cccccatcgc ggctttccgg gcccaagcca agcagctcgg cgtcggggcg
     7861 cgagtccatt tccttggcag cctgcgggac atggtgcccg cctataccgc cgccgacctg
     7921 ctggtccatc ccaccaccga ggatacctac gcgatggtgg tcctcgaagc cctcgcccac
     7981 ggtctgccgg tgatcgtcag cggacccgcc cactgcggca tcgcgacaga actgcgggac
     8041 ggggtggacg cgctgctcgt ggacgacccg cgggacgcgg acgccctcgc ccgcgccatc
     8101 gcgcgggtgc gtaccgatgt gggcctggcg gcggccctcg gtctggccgg gcgtgcgttc
     8161 gccgccgcac gcgactggga cgcccgcgcc gcggttcagg aagccctgta tttcgaggca
     8221 gcgggcaacg cgacgggctg agtccgggct ccgcgcgatc cgactgggtt cgggaaaagt
     8281 ggaccggcag accgccgttc ggggcgccac cttgccggac gaacgaaggg gcaggtcgat
     8341 gcccgccctg ccacctcacg tggatccgcc gcaccgggcc accggggccc ggtggccacg
     8401 ctgcggacgg gcctcagccc agcttctgct tcagcagcgc attcacctgg gtcgggttgg
     8461 ccttgccctt gctggccttc atgcactggc ccacgagggc gttgaaggct ttttccttgc
     8521 cggcgcggta ttcgtccacc gatttcgggt tggcggcgag gacttcgtcg atgagggcct
     8581 cgatggcgct gctgtcggtg acctgcttga ggccctgggc gtcgatgatg gcgtcggcgg
     8641 aggggccttc gccgttccac agggcctcga agaccttctt gccgatggcg ttggagatgg
     8701 tgttgtcggc gatgcgctgc accaggccgg c
//
LOCUS       DAOQPA010000133         8489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4571_length_8489_cov_11.834361, whole genome shotgun sequence.
ACCESSION   DAOQPA010000133 DAOQPA010000000
VERSION     DAOQPA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_8489_cov_11.834361"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            672..4925
                     /locus_tag="PKX01_14700"
     CDS             672..4925
                     /locus_tag="PKX01_14700"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HMZ77430.1"
                     /translation="MTLAIDTILSIASATPSLLEGNGASTAFEFTVTRSGSATGAVSA
                     TWTVGGAAIGAEDFVGGVLPGGVVNFADGETTKTITVNVAGDLGVEADEAFFVKLTGA
                     TGALIGTDTASAVILNDDTALGIAALTGAQAEGNSGSTPFTFTVTRSGNLSGSASAAW
                     AVSGGSANATDFVGGVLPTGTVTFAAGESSKTVTVNVVGDTVGEADETFNVTLANVTG
                     ATVTTATASATILNDDALLNIATVTASAAEGSSGSTPFGFTITRSGNTAGVSSVKWAV
                     SGGSAVAADFSGSVLPSGTVSFAAGETTKTITVNVAGDTALEADESFNVVLSAASGAT
                     LGTATASALILNDDAQLAIAAASANMAEGTGASATPFTFTVTRSGYLGAAASANWAVS
                     GGTATAADFTGGVLPTGTVNFAPGESSKTVTVNVAADSLVESNETFNVVLSGATGASL
                     GTTTATGTIVTDDTQLDIKAGVASKAEGNTGSTPLTFTVTRSGITTGASTANWAVSSA
                     TADATDFTGGVLPSGTVSFAAGETSKTLTVNVAGDNLIESDETFAVTLSGATGATLGA
                     ASASTTILNDDATMSIAAADARKAEGSGSTATPFTFTVTRSGSLIGASTVKWAVSGGT
                     ATATDFSGNTLPTGTVSFAAGETSKTITVNVAADSLVEADETFNVVLSTPTGGVIGTG
                     TASGTLLNDDAQLAIAATSASKAEGTGATATPFTFTVSRSGNLAGTSTANWAVSGGTA
                     TAADFSGSVLPTGTVSFAAGESSKLITVNVAADSLVEANETFNVVLSAPSAGTSLGTA
                     SAAGSIVNDDTLVSLATSAVSKVEGNSASTAFTFTVSRTGVTTGTSSVGWAVTGGTAS
                     AADFTGGALPTGTVSFAAGETSKTLTVNVAGDNLIEGDETFTVGLTTATGATIGTATA
                     NATILNDDALVGIAATSAVRSEGNSGSTPFTFTVTRSGTLTAASTVNWAVSGGAVTGA
                     DFTGGALPTGSVSFAAGETAKTITVNVAGDGNFEGDEAFTVSLTGTSGAQVDATAASA
                     TGTILNDDGAVDITADTSRFVEGNVGSSTASFTLTRRINTSNTASINWAVSGGTATTS
                     DFIGGVLPSGTVSFAAGETTKTITVNTVADTLYETDESFNVVLSGGVGTGIGIGSATT
                     VILNDDPVLNIAAINPAQVEGSGGGSTPFAFAVSRGGNLTGTMAVRWAVASPNASGAD
                     FVAGVLPSGTITFADGEALKIINVDVLADDLPENDKSFLVGIAGPFGVSVGKSSATVT
                     LVDDDNYLVTDVASPTVVLQGTMADDVLTGGAGPERIIGLAGKDTLTGGGGADQFLIT
                     APSDGPHTITDFSTADGDRIQILATNFGLIPNSTPVLLSGSTLPTPSTSGGQFLYNAS
                     NGALWFDRDGADSGFAAVQVATLSNRPTLSASDLQLVLA"
     gene            5023..7179
                     /locus_tag="PKX01_14705"
     CDS             5023..7179
                     /locus_tag="PKX01_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HMZ77431.1"
                     /translation="MDSLTPFETALRSLTAVARHHRVNTSPEQIRRAHPAAAGTPADA
                     LPTLVAAARAIGLKARSLKTDFPALPPLQDTFPALARLNNGNWVVLVGLRGQGEEAAV
                     AVLDPLATPADAVFLIDRARFEAAWSGDLVLVTRVYSLHDENRPFGLGWFVPELLRQK
                     AFLRDVAVAALVLHVLALAMPVFTQLVIDKVIVHQGYSTLYVLTAGVILALLFEAAFS
                     YLRQTFMLQATNRLDVRLARKTFAHLLRLPITFFETGTAGVITRHMQQAEKIRQFLTG
                     RLFSTLLDASVLVLLLPVMFFYSGLLATLVLAFAAAIGAVVVALIPAFRARLQALYSA
                     EAERQSLLVETIQGMRTVKALAVEPLRRDDWDARSARTVALHFRVGRISLAAHTAMSL
                     LEKLMGVSVIAVGANLVFDQTLSVGSLIAFQMLSGRVVSPLVQIVALVHEYQETALSV
                     RMLGEIMNRPVEGQRASGGLEPTLEGHIAFENVSFRYAPGAAAALEQISFSLPAGKVV
                     GVVGRSGSGKTTLTRLMQGLYPVQDGIVRFDGIDLREIDLAHLRRQIGVVLQDNFLFR
                     GTVRDNLSITRPTAGFDEIVAAARLAGADEFIERLPQGYDTLLEENAANLSGGQRQRL
                     AIARALLNRPRILILDEAASALDPESEAIFLNNLAGLTAGRTVIIVSHRLSALTRADA
                     IMVLDRGHIVDAGRHEELLGRCAVYTQLWNQQTRHL"
     gene            7182..>8489
                     /locus_tag="PKX01_14710"
     CDS             7182..>8489
                     /locus_tag="PKX01_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77432.1"
                     /translation="MDRRTLRAALTDFLPEVHALEHAEPPHGPRVLLYALLALVVTAL
                     VWASVSEIDILAIGRGRLVTPLPNLVVQPLEAGILKSIDVRVGQQVRQGTVLATLDPT
                     FAQADAGQLGARRDSLAQRAQRLEAELHGNPATPSTAAGQEGVQARLMAERQAAHAAR
                     LKQYDESIARLRAALETNRRDEATLAERVHSLAELEAMQAELEARQFGSRAKVLELRE
                     RKLEVERDRNIAAHRDQELRREIAAAEAERTGYLRTWRQDSLEKLSQTVQERDEVTAQ
                     LDKTRLRTDLIRLTAPQDGVVLEIGKRSVGSVLKEAEPLFTLVPMNAPLEAEVEIGPG
                     DVGLIRPGDTVRIKFDTYPFQKHGTARGRVLHVSPDAFSRQGPGGATTSYYLARVALD
                     DTRLDGIAQPTLLLPGMSLTGEIVVGRRSVIAFFLYPLIRTLDE"
BASE COUNT         1408 a         3280 c         2568 g         1233 t
ORIGIN      
        1 ctcccgtcat cgttgatcga tggcgggacg ccgcacgcct ccccctcacg ccccgccgcc
       61 gatcgcctcc caggcgattc tcctctcccg catcacgcgc gcgctgcgca catcacgacc
      121 ccagcccggc atctccggcg catcgacgtg acgcccgccc cttcctggcg cacccacctg
      181 gaatcaaccg cccggggatt gccgcacgcg ttgcgcacga ccgcccgccc cgaggcccga
      241 gcccgtccag ccacggcccg cgcgccccac gtcggcctcc gggggagtgc cgcccgcccc
      301 gcgtaaattc agaacaatct gatacacatc aagcttctac cgacgcaatt cgcgtaaaac
      361 gatgctgtaa gattcacaat ttaacatcaa ccccattgac aaatacatga atgtcatgaa
      421 ctgaaaccca gcccccgcgc cgattcaggc gggccgcacg ggcgccttcg gcacgcgatc
      481 ccactggggc ctgcatcaac cggcttgcgg tcggggcgca tccggattcc ggatttttcc
      541 gaatccggcc aaggaggaat tacgtcacga accgcaatcg gagacatccg ccttgcaggc
      601 atgtcgggat ttacacttgt ttgctttccg catacacctt ttttcgtcca ccgtcccctg
      661 atctgaccac catgaccctc gccatcgaca ccatcctgag tattgcctcc gcaacgccca
      721 gcctgcttga aggcaatggc gccagcacag cgttcgaatt cacggtgacc cgcagcggca
      781 gtgccacggg ggccgtcagc gccacctgga cggttggcgg agcggcgatc ggtgcggaag
      841 atttcgtcgg cggcgtcctg cccggcggcg tcgtcaattt cgccgacggc gagaccacca
      901 agacaatcac cgtgaacgtc gccggtgatc tgggtgtcga ggcggacgaa gcattcttcg
      961 tgaagctcac gggcgccacc ggcgcgctca tcggcaccga caccgcctcg gcggtcattc
     1021 tcaacgacga caccgccctg ggcatcgcgg cgctgacggg tgcgcaggcc gaaggcaaca
     1081 gcggcagcac gcccttcacc ttcaccgtga cccgcagcgg caacctgagc ggcagcgcca
     1141 gtgcagcctg ggccgtcagc ggcggcagcg ccaacgcaac agacttcgtc ggcggcgtgt
     1201 tgccgaccgg tacggtcacc tttgcggcgg gcgagagcag caagacagtc accgtcaacg
     1261 tcgtcggcga cacggtcggc gaggccgacg aaaccttcaa cgtcacgctg gccaacgtca
     1321 ccggcgccac cgtgaccacc gcgacagcca gtgccacgat cctcaacgac gacgccctcc
     1381 tgaacatcgc caccgtcacc gcctcggccg ccgagggcag ttccggcagc accccgttcg
     1441 gcttcacgat cacccgcagc ggcaacaccg ccggtgtctc gtcggtcaag tgggccgttt
     1501 ccggtggcag cgccgtggcg gccgacttca gcggcagcgt cctgccgagc ggcaccgtca
     1561 gctttgccgc cggcgaaaca accaagacga tcaccgtcaa cgtggccggc gacaccgccc
     1621 tggaagccga cgaaagcttc aacgtggtgc tctcggcggc cagcggcgca acgctcggaa
     1681 ccgcgacggc cagcgccctc atcctcaacg acgacgccca gctcgccatc gccgccgctt
     1741 cggccaacat ggcggagggt accggcgcgt cggcgacgcc cttcaccttc accgtcaccc
     1801 gcagcggcta cctgggcgcc gcagcctcgg cgaactgggc cgtgtcaggc ggcaccgcca
     1861 ccgctgcgga tttcaccggt ggcgtcctgc ccaccggcac ggtgaatttc gcccccggcg
     1921 agagcagcaa gaccgtcacc gtaaacgtgg ccgccgacag cctggtggag tccaacgaga
     1981 ccttcaacgt ggtgctgtcc ggcgccacgg gcgccagcct gggcaccacc acggccaccg
     2041 gcaccatcgt cactgacgac acccagctcg acatcaaggc cggtgtagcc agcaaggccg
     2101 aaggcaatac gggcagcacc ccgctcacct tcaccgtcac ccgcagcggc atcaccaccg
     2161 gcgcatcgac ggccaactgg gccgtgtcca gcgccacggc cgatgccacc gacttcaccg
     2221 gtggcgtgct gccgagcgga accgtcagct ttgccgcggg cgaaacctcc aagaccctca
     2281 ccgtcaatgt ggcgggcgac aatctcatcg aatccgacga gaccttcgcg gtcaccctct
     2341 ccggcgcgac gggtgcgacc ctgggcgcgg ccagcgccag caccacgatc ctcaatgacg
     2401 acgcgacgat gagcatcgcc gccgccgacg cacgcaaggc cgagggcagc ggcagcaccg
     2461 ccaccccatt caccttcacc gtcacccgca gcggctcgct cattggcgcc tcgacggtca
     2521 agtgggccgt cagcggcgga acggccacgg ccaccgactt ctccggcaac accctgccga
     2581 ccggtactgt cagcttcgcc gccggcgaaa cctccaagac catcacggtc aatgtcgccg
     2641 cagattcgct ggtggaagcg gacgagacct tcaacgtggt cctgtccaca ccgaccggcg
     2701 gcgtcatcgg cacgggcacc gcgagcggca cgctcctcaa cgacgacgcc cagctcgcca
     2761 tcgccgccac atccgccagc aaggccgaag gcacgggcgc caccgccacg ccgttcacct
     2821 tcaccgtgag ccgcagcggc aacctggccg gcacctccac ggccaactgg gccgtctccg
     2881 gcggcaccgc cacggcggcg gacttcagcg gcagcgtgct ccccaccggc acggtgagct
     2941 tcgcggccgg cgagagcagc aagctcatca cggtaaacgt ggctgcagac agcctcgtgg
     3001 aagccaacga aaccttcaac gtggtgctgt cggcgccgtc ggccggaacc agcctgggta
     3061 cggccagcgc cgcaggctcc atcgtcaatg acgacaccct ggtttccctc gccaccagtg
     3121 cggtcagcaa ggtcgaaggc aacagcgcca gcacggcctt caccttcacg gtctcccgca
     3181 ccggcgtcac cacgggcacg tcctcggtag gctgggcggt caccggcggc accgcttctg
     3241 cggccgactt caccggcggc gcgctgccga ccggcactgt cagctttgcc gcgggcgaga
     3301 cctccaagac gctcaccgtc aatgtggcgg gcgacaacct catcgaaggc gacgaaacct
     3361 tcaccgtcgg tctgaccacc gccaccggcg cgaccatcgg caccgccact gccaacgcca
     3421 cgatcctcaa cgacgacgcc ctggtcggga tcgccgcgac gagtgccgtg cgcagcgaag
     3481 gcaacagcgg cagcacgccc ttcaccttca ccgtcacccg cagcggcacc ctcaccgccg
     3541 cctccaccgt caattgggcg gtctcgggcg gcgccgtcac gggcgccgat ttcacgggcg
     3601 gggcgctgcc gaccggcagc gtgagcttcg cggccggcga gacggccaag acgatcaccg
     3661 tcaacgttgc gggcgatgga aatttcgaag gcgacgaagc cttcaccgtg agcctgaccg
     3721 gcaccagcgg cgcccaggtg gacgcgactg ccgcatccgc caccggcacg atcctcaatg
     3781 acgacggcgc cgtggacatc actgccgaca cgagccgctt cgtggagggg aacgtgggca
     3841 gctcgaccgc ttccttcacc ctgacgcgcc gcatcaacac aagcaacacc gccagcatca
     3901 actgggccgt ctccggcggc acggccacca cctccgactt catcggcggc gtcctgccct
     3961 ccggcacggt ctccttcgcc gccggcgaga ccaccaagac catcaccgtc aacacggtgg
     4021 ccgataccct ctacgaaacc gacgaatcct tcaatgtcgt gctgtccggc ggcgtgggca
     4081 ccgggatcgg catcggctcc gccacgacgg tcatcctgaa cgacgatccg gtcctcaaca
     4141 tcgcggcgat caacccggcc caggtcgaag gctccggagg cggttccacc cccttcgcct
     4201 tcgccgtctc gcgcggcggg aacctcaccg gcaccatggc cgtacgctgg gcggtggcca
     4261 gcccgaacgc ctccggtgcc gatttcgtcg ccggcgtcct gccgagcggc accatcacct
     4321 ttgccgacgg cgaagcgctc aagatcatca acgtcgatgt gctcgccgac gacctcccgg
     4381 agaacgacaa gtccttcctg gtcggcatcg ccggcccctt cggcgtgtcg gtcggcaaga
     4441 gttcggcgac ggtgaccctc gtggacgacg acaactacct ggtgaccgac gtggcgtccc
     4501 ccaccgtggt cctccagggg accatggccg acgatgtgct gaccggcggc gccggacccg
     4561 aacggatcat cggcctggcg ggcaaggaca ccctgaccgg cggcggcggc gcggaccagt
     4621 tcctgatcac cgcgccgagc gatggtccgc acacgatcac cgacttctcc acggctgacg
     4681 gcgacaggat ccagatcctc gccaccaact tcggcctcat tcccaattcc acgcccgtcc
     4741 tgttgagcgg ctccaccctg ccgacgccca gcaccagcgg cggccagttc ctgtataacg
     4801 cgagcaatgg cgcgctgtgg ttcgaccgcg atggcgccga cagcggcttt gccgcggtcc
     4861 aggtggccac cctcagcaac cggcccaccc tgagcgccag cgacctccag ctcgtgctgg
     4921 cctgagcatc ctcctgcggg acaggaccag gccctcgggc ccggtccagc cctgcttgcc
     4981 ggccctcgtt ccctgctgac catcacggga caccgtccat cgatggattc cctcacgccc
     5041 tttgagacag cactcaggag cctcaccgcg gtggcccggc accaccgcgt caacacctct
     5101 cccgagcaga tccggcgcgc ccatccggcg gcggccggca cgccggccga cgcgctcccg
     5161 acgctggtgg cggcggcccg cgccatcggg ctcaaggcgc gcagcctgaa gaccgacttc
     5221 ccggcgctcc cgcccctgca ggacaccttt ccggccctcg cccgcctcaa caatggcaac
     5281 tgggtcgtgc tggtcggact ccgcgggcag ggcgaggagg ccgcggtggc cgtgctcgac
     5341 ccgttggcca ccccggcgga cgcggttttc ctgatcgatc gggcgcgctt cgaggccgcc
     5401 tggtccggcg atctcgtgct ggtcacgcgc gtgtacagcc tccatgacga gaaccggccc
     5461 ttcggactgg gctggttcgt cccggagctc ctccgccaga aggcgttcct gcgggacgtg
     5521 gccgtcgccg ccctggtcct gcacgtgctg gccctggcca tgcccgtgtt cacccaactg
     5581 gtcatcgaca aggtgatcgt gcaccagggc tactccacgc tctacgtgct gacggccggc
     5641 gtaatcctcg ccttgctgtt cgaggcggcc ttctcctacc tgcgtcagac cttcatgctc
     5701 caggccacca accggctgga cgtccggctg gcccgcaaga ccttcgccca tctcctgcgc
     5761 ctgcccatca ccttcttcga gacgggcacc gccggcgtca tcacccgcca catgcagcag
     5821 gccgaaaaga tccgccagtt cctcaccggc cgcctcttct cgaccctgct cgacgcctcg
     5881 gtgctggtgt tgctgctgcc ggtcatgttc ttctactccg gactgctggc caccctggta
     5941 ctggccttcg ccgccgccat cggcgccgtc gtggtggccc tgatcccggc cttccgtgcc
     6001 cgcctgcagg ccctgtattc ggccgaggcg gaacgccagt cgctgctcgt ggaaaccatc
     6061 cagggcatgc gcaccgtcaa ggccctggcg gtagagcccc tgcgccgcga cgactgggat
     6121 gcccgctccg cgcgcacggt ggcgctgcac ttccgcgtgg gccgcatctc cctggccgcc
     6181 cacacggcca tgagcctgct cgaaaagctg atgggcgtca gcgtcatcgc cgtgggcgcc
     6241 aacctggtct tcgaccagac cctcagcgtc gggtccctga tcgccttcca gatgctctcc
     6301 gggcgggtcg tgtcgcccct cgttcagatc gtggcgctgg tccacgaata ccaggagacc
     6361 gccctgtccg tgcgcatgct cggcgagatc atgaaccgtc cggtcgaagg ccagcgggcc
     6421 tccggcggcc tggagcccac cctggagggg cacatcgcct tcgaaaacgt cagcttccgt
     6481 tacgccccgg gtgcggcggc ggccctggag cagatcagct tcagcctgcc cgccggcaag
     6541 gtggtgggcg tcgtcggccg cagcgggtcg ggcaagacca ccctcacccg cctgatgcag
     6601 ggcctctatc cggtgcagga cggcatcgtc cgcttcgacg gcatcgacct ccgcgagatc
     6661 gacctcgccc acctgcgccg ccagatcggc gtggtgctgc aggacaactt cctgttccgc
     6721 ggcacggtgc gcgacaacct gtccatcacg cggcccactg ccggcttcga cgagatcgtc
     6781 gccgccgccc gcctggccgg cgccgacgag ttcatcgaac gtctgcccca gggctacgac
     6841 accctgctgg aagagaacgc cgccaacctc tccggcggcc agcgccagcg cctggccatc
     6901 gcccgcgccc tcctcaaccg gccgcgcatc ctgatccttg acgaggctgc aagcgccctc
     6961 gacccggaat ccgaggccat cttcctgaac aatctggccg gactcaccgc gggccgcacg
     7021 gtgatcatcg tctcccaccg cctgtccgcc ctcacccgcg ccgacgcgat catggtcctc
     7081 gaccggggcc acatcgtgga tgccggtcgc cacgaggaac tgctgggccg ctgcgcggtg
     7141 tatacccagc tctggaacca gcagacccgg cacctgtgac catggaccgc cgcaccctcc
     7201 gcgcagccct caccgacttc ctgccggagg tccacgcgct ggaacacgcc gaaccacccc
     7261 acggtccgcg cgtcctcctc tacgccctgc tcgccctggt ggtcacggca ctggtgtggg
     7321 ccagcgtctc cgagatcgac atcctggcca tcggtcgcgg ccgcctggtc acgcccctgc
     7381 ccaatctggt ggtgcaaccc ctggaggccg gcatcctcaa gagcatcgac gtgcgcgtcg
     7441 gtcagcaggt gcgacagggt acggtgctcg ccaccctcga cccgaccttc gcccaggccg
     7501 acgccggtca gctcggcgcc cgccgcgaca gcctcgccca gcgggcgcag cgcctggagg
     7561 cggaactcca cggcaacccg gcgaccccca gcactgcggc gggccaggaa ggcgttcagg
     7621 cccgcctgat ggcagagcgc caggccgccc acgccgcgcg cctcaagcag tacgacgaaa
     7681 gcattgcccg cctgcgtgca gcgctagaga cgaatcgccg cgacgaagcc accctggctg
     7741 agcgcgtcca cagtctggcc gagctggaag ccatgcaggc cgagctggag gcccgccagt
     7801 tcggctcccg cgccaaggtt ctggaactgc gcgaacgcaa gctggaggtg gaacgcgacc
     7861 gcaacatcgc cgcccaccgg gaccaggaac tgcgccgcga gatcgcggcg gccgaggccg
     7921 agcgtaccgg ctacctgcgt acctggcgcc aggattccct ggaaaagctc tcccagactg
     7981 tccaggagcg ggacgaggtc accgcccagc tggacaagac ccgcctgcgc accgacctga
     8041 tccgcctcac ggcgccccag gacggggtgg tgctggaaat cggcaaacgc tcggtcggct
     8101 ccgtcctcaa ggaggccgag cccctgttca ccctggtccc aatgaacgcg cccctggagg
     8161 ccgaagtgga gatcggcccc ggcgacgtgg gcctgatccg gccgggcgac accgtgcgca
     8221 tcaagttcga cacctacccg ttccagaagc acggcacggc ccgcgggcgg gtactgcacg
     8281 tgagtcccga cgccttttcg cgccagggcc ccgggggcgc cacgacgtcc tactacctgg
     8341 cccgggtcgc cctggacgac acccgcctgg acgggatcgc ccagccgacg ctgctgctgc
     8401 cgggcatgag cctcaccggc gagatcgtcg tcggccgacg ctcggtgatc gccttcttcc
     8461 tctatccgct gatccgcacc ctcgacgaa
//
LOCUS       DAOQPA010000134         8354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4677_length_8354_cov_20.019641, whole genome shotgun sequence.
ACCESSION   DAOQPA010000134 DAOQPA010000000
VERSION     DAOQPA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_8354_cov_20.019641"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="PKX01_14715"
     CDS             complement(<1..1856)
                     /locus_tag="PKX01_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMZ77433.1"
                     /translation="MTARSPAPDSIPSRGNPLPELRQAIVALTRAPEAECMARLFGDA
                     RMAPEALARVCARARQLAEGVRAGRVRAGGVDALMHEFALGSAEGVALMCLAEALLRV
                     PDAAGRDALIRDKLHARDWSAHVGHSPSLFVNAAAWSLALTGRLLSADESARLPGTLA
                     RMATRGGEAVVREAMEFAMRRVGRQFVMGETIEEALERVASGAAADYRYSFDMLGEAA
                     LTADDATRYAAAYAHAIHAIGGADHWRGVRAGNGISIKLSALHPRYTWSQRDRVMAEL
                     LPRVHHLCVLARGHDIGLTIDAEESDRLELSLDLFAALATDPDLRDWAGLGMVVQAYQ
                     KRAPAVIDWLIALGRRSGRRIMTRLVKGAYWDGEIKRAQQDGLADYPVYTRKVHTDTA
                     YLACARRMLAAPDAIYPAFATHNAHTVAAVLEMAGPFHDGAYEFQCLHGMGETLYRQL
                     VAGADPALRRPVRVYAPVGSHETLLAYLVRRLLENGANTSFVHRVVDDSVPLDMLVAD
                     PVERAVAQGGAPHPAIPLPGALFVERANSRGFDLAAAPVRRAFLAALEKSRASTFTAV
                     PRVVGEAAAGDPSPSIQIRNPARRSDFVGTAACADAWQIEAALAAAAGAAAG"
     gene            complement(1968..2969)
                     /locus_tag="PKX01_14720"
     CDS             complement(1968..2969)
                     /locus_tag="PKX01_14720"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMZ77434.1"
                     /translation="MRRVTLTQFLIEQQRKGLMGADLRLLMEVVARAVKAIAVNVSKG
                     ALAGVLGEAGSDNVQGEAQKKLDVIANDILIQANEWGGHLAAMASEEVEEIRPISGEY
                     PKGAYLLLFDPLDGSSNIDVNISVGTIFSVLRCPEGVSAPTEADFMQPGTAQVAAGYS
                     VYGPSTQLILTVGDGVHGFTLDREMGSFIYTHPNMTIPADTKEYAINASNARFWEAPV
                     QRYIGELQAGKEGPRGKDFNMRWVASMVADVHRILTRGGIFMYPMDSKMKDKGGKLRL
                     MYEANPMAMIVEAAGGAATTGRERILDIQPSRLHQRVPVVLGSKNEVERVTAYHLEG"
     gene            3114..4376
                     /locus_tag="PKX01_14725"
     CDS             3114..4376
                     /locus_tag="PKX01_14725"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HMZ77435.1"
                     /translation="MKVLVIGAGLEGVTSAWYLAQAGCEVTVVERRPGPGLETSFANG
                     GQISVSHPEPWANPRAPGTILRWIGRDDAPLLFRPSLDGRQWRWGLAFLRECLPARTA
                     RNTAAIASLACYSRDQLRALRDATGLEYAAQTQGILHLFTTSRELVHARDHERILSRL
                     GIRANLLTSAQAISLEPALAPLADRLKGALHGVDDESGDAHLFCRALAERAAAAGVTF
                     LYDSAVYSMETLAGRVAGVRVLDSARRAGVLTADAFVVAAGSYSPALLARVGEALNIY
                     PVKGYSVTAQIVDPERAPHLSLTDESRRVVCSRFGDRLRVAGTAELNGFDLSPNPARS
                     NALVRWLEEHFPGVTHSRKAEHWCGLRPATPSNVPIIGRGRYDNLFLNAGHGTLGWTL
                     ACGSARALADIITGRKPEPEFPFRGLAR"
     gene            complement(4378..5253)
                     /locus_tag="PKX01_14730"
     CDS             complement(4378..5253)
                     /locus_tag="PKX01_14730"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HMZ77436.1"
                     /translation="MTFVRVGIDEIEIGQPLRWPLYDDKKQPLLARGAVVENAERKAA
                     LFGQGVFRKTVEDERPTGEGGERPAEDLRGLDEIRMDIGDTLQLQAQSETSQSRHYVK
                     LIGYLKGKSVVVTTPTQDGKVLLMREGQAFVVRMFSGKSVYAFGATIFKVANVPYPHL
                     HLTYPSQVRGLVVRRGARARVNLIAAVSGADGRSHAGTVVDLSIGGAMLVAKSPIGLK
                     DDRIKVKFRVVVSDVEQYLTLDAVLRTVHATAATEGEPSMVNHGLQFDSLPHAEQVVL
                     SAYVYRVLFEESTEG"
     gene            complement(5343..5843)
                     /locus_tag="PKX01_14735"
     CDS             complement(5343..5843)
                     /locus_tag="PKX01_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ77437.1"
                     /translation="MAAMQWTDKLALGLGPMDDTHKEFVDHYNRLLQVTGEALLTELD
                     AFIAHTVAHFDQENRWMEQVDFPACHKAEHDRVLAVVADVRKRMERGDEALGRQLIQE
                     LPIWFDNHVATMDAALAAYLESIGYDTATGEIHRPPQVDACGNPVEAGACCTPAPVSP
                     VEGSPA"
     gene            complement(5929..6366)
                     /locus_tag="PKX01_14740"
     CDS             complement(5929..6366)
                     /locus_tag="PKX01_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HMZ77438.1"
                     /translation="MSEVSTKPYLIRAIFQWCVDQGLTPYLSVHVDGRTQVPRAFVQD
                     GQIVLNVSMDATQNLVMGNELIAFQARFGGVAHHIAVPVDNVSAIYARENGHGMAFEV
                     NPSDPAEASEPDATASESPPATADTPPDDGPKGGARGRLKLVK"
     gene            complement(6420..7016)
                     /locus_tag="PKX01_14745"
     CDS             complement(6420..7016)
                     /locus_tag="PKX01_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ77439.1"
                     /translation="MMNLYSGTTDPFSHRCRIVLFEKGMDFQVIDVDLFNKPEDIAVI
                     NPYNRVPVLVDRDLILYESNIINEYIDERFPHPQLMPPDPIMRARARQLLHTFELELF
                     SHVEALEKNAKEADKERAHIRDQLTQLAPIFTKQKFLLGEEFSMLDVAIAPLLWRLDH
                     YGIELPRTAAPLMKYAERIFSRQGFIDAMTPSEKVMRR"
     gene            complement(7124..7891)
                     /locus_tag="PKX01_14750"
     CDS             complement(7124..7891)
                     /locus_tag="PKX01_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HMZ77440.1"
                     /translation="MKTRGLSLVKKLFATALFAPLLAVASGPELHLDKAPVSSDPAAL
                     QNGAKLFVNYCLNCHGASYLRYNRLKEIGLSDQMIKDNLMFTADKIGEPMRIAMQRDE
                     AKAWFGAAPPDLTLIARSRASEFGSGADWLYTYLRSFYRDPGRPTGWNNTVFENVGMP
                     HVLYELQGDQVAKFEEHDDGHGNMTKVVHIEPGKPGKMSKDEYDKATADLVSFLVWMG
                     EPMAGTRKTIGTFVLAFLGVLFVLSYLLKKNYWKDVH"
     gene            complement(7888..>8354)
                     /locus_tag="PKX01_14755"
     CDS             complement(7888..>8354)
                     /locus_tag="PKX01_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HMZ77441.1"
                     /translation="IVFFAPEGGGYFLEFNNFIPADPLKTPPHIAPVWYFTPFYSILR
                     AVTYPLFGIDAKFWGVVAMGAAVIIIAFLPWLDRSPVKSIRYKGGLFKAILAVFIVAF
                     FILGYLGVLPPTPGRTLVSQICSVIYFGFFLLMPWYSKIDKTKPEPERVTWK"
BASE COUNT         1507 a         2708 c         2710 g         1429 t
ORIGIN      
        1 cagccggcag ccgcgcccgc cgcggcggcc agcgcggctt cgatctgcca cgcatcggca
       61 caggctgcgg tgccgacgaa gtcgctgcgg cgggcaggat tccggatctg gatggacggg
      121 gacggatcgc ccgcagcggc ctcaccgacc acccgcggca cggccgtgaa ggtgcttgcg
      181 cggctcttct ccagcgctgc gagaaaggcc cgccggaccg gcgcggccgc cagatcgaag
      241 ccgcggctgt tggcacgctc gacgaacagg gcgcccggta gcgggatggc cggatggggc
      301 gccccgccct gggccaccgc ccgttcgacc gggtcggcga ccagcatgtc gagggggacc
      361 gagtcatcca cgacacgatg cacgaagctg gtgttggcgc cgttctccag gaggcgccgc
      421 accagatagg ccagcagggt ttcgtggctg ccgaccggcg cgtacacccg cacgggacgg
      481 cgaagggcgg gatccgcacc ggccaccagc tggcgataga gggtttcgcc catgccatgc
      541 agacactgga actcgtaggc gccgtcgtgg aacggacccg ccatctcgag gaccgccgcg
      601 accgtatggg cgttgtgggt ggcgaaggcc gggtagatcg cgtccggcgc tgccagcatg
      661 cggcgggcac aggccaggta ggcggtgtcg gtgtggacct tgcgggtgta gaccgggtaa
      721 tcggcgaggc cgtcctgctg ggcgcgcttg atttcgccat cccagtaggc gcccttgacc
      781 aggcgggtca tgatgcgccg cccgctgcgc cgcccgaggg cgatgagcca gtcgatgact
      841 gccggcgcgc gcttctggta ggcctgcacg accatgccca gccccgccca gtcgcgcaga
      901 tccgggtcgg tggccagcgc agcaaagaga tcgagggaca gctcgaggcg gtcggactct
      961 tcggcgtcaa tggtcaggcc gatatcgtgc ccccgcgcca ggacgcacag gtgatgcacc
     1021 cgtggcagga gttcggccat cacccggtcc cgctgggacc aggtgtagcg tggatgcagg
     1081 gccgacagtt tgatggagat gccattgccg gcgcgcacgc cgcgccagtg atcggccccg
     1141 ccaatggcgt ggatggcatg ggcgtaggcg gcggcgtagc gggtcgcgtc gtcggcggtg
     1201 agcgctgcct caccgagcat gtcgaaggag tagcgatagt ccgccgcagc gccgctggcg
     1261 acgcgctcca gcgcctcctc gatggtttca cccatcacga actgccgacc aacccggcgc
     1321 atggcgaatt ccatggcttc ccggaccacc gcctcgccgc cgcgggtggc catccgggcc
     1381 aacgtgccgg gcaggcgcgc ggactcatcg gcggacagca gccggccggt cagcgccaga
     1441 ctccaggcgg cggcattgac gaagagactc gggctgtggc cgacgtgcgc ggaccaatcg
     1501 cgggcgtgca gcttgtcgcg aatcagcgcg tcccgccccg ccgcatcggg taccctcagc
     1561 aaggcttcgg cgaggcacat cagcgcaacc ccctcggcgc tgccaagggc gaattcatgc
     1621 atcagggcat cgacacctcc tgcccggaca cgccctgccc gaacgccctc ggccagttgc
     1681 ctcgccctgg cgcagacccg cgccagcgcc tccggcgcca tccgggcatc accaaagaga
     1741 cgcgccatgc attcggcttc gggtgctcgg gtgagcgcga caatcgcctg gcgcaactcg
     1801 ggcaagggat tgccgcgcga cgggatggaa tcgggggcgg gcgaacgggc agtcatggca
     1861 tcaccggtac ggctcaaaga aaagggggcg ctccgttcga ccggaaaaac gccccgcgat
     1921 tccaggcctg gccacacacc gcagggtggt ggcctgccga cgcatagtca gccttccagg
     1981 tggtaggcgg tgacacgctc cacttcattc ttcgacccca gcacaaccgg gactcgctgg
     2041 tgcagccggg aaggctggat gtcgaggatg cgttcccgcc cggtggtggc ggcgccgccc
     2101 gcagcttcga cgatcatcgc catgggattg gcttcgtaca tcaggcgcag cttgccgccc
     2161 ttgtccttca tcttgctgtc catcgggtac atgaaaatgc ccccgcgggt caggatgcga
     2221 tgcacgtctg cgaccatcga ggccacccag cgcatgttga agtccttgcc acgggggcct
     2281 tccttgcccg cctgaagctc gccgatgtag cgctgcaccg gcgcttccca gaagcgcgca
     2341 ttggacgcgt tgatggcgta ttccttggtg tcggcgggaa tcgtcatgtt cggatgggtg
     2401 tagatgaagc tgcccatctc gcgatcgagg gtgaacccat gcacgccatc accgacggtg
     2461 agaatgagct gcgtggaggg gccatacacc gagtagcccg ccgccacctg ggccgtgccg
     2521 ggctgcatga aatcggcttc cgtcggcgcg gaaacgcctt ctgggcagcg caacaccgag
     2581 aagatggtcc cgacggagat gttcacatcg atgttggacg aaccgtcgag ggggtcgaac
     2641 agcagcaggt aggcaccctt cgggtattca ccggaaatcg gccggatctc ttcgacctcc
     2701 tcggatgcca tggccgccag gtggccgccc cactcgttgg cctgaatcag gatgtcattg
     2761 gcgatcacat ccagcttctt ctgggcctcg ccctggacgt tgtcactgcc cgcctccccg
     2821 agcacaccgg ccagcgcacc tttggagaca ttgacggcga tggccttgac cgcccgcgcc
     2881 acgacttcca tgagcagccg caggtcggcg cccatcaggc ccttgcgctg ttgttcgatc
     2941 agaaactggg tgagcgtgac acgacgcatg gcgtatttct cctgagcaat tccggtaaag
     3001 tccggattat cgcccggaat gcgctgctgc gtcgtttatg attacagggt tcgaagcatc
     3061 gacaccctct ttggcggtgg cgccagccgc cccggaaata ttgtttcagc atcatgaaag
     3121 ttctcgtcat cggcgccggc ctggaaggcg tcaccagcgc ctggtatctg gcccaggcag
     3181 gttgcgaagt caccgtcgtg gaacgtcgtc ccggccccgg cctggaaacc agctttgcca
     3241 acggcggcca gatctccgtg agccatccgg aaccctgggc caatccgcgg gcaccgggga
     3301 cgatcctgcg ctggatcggt cgtgacgacg cccccctgct gttccggccc agcctcgatg
     3361 gtcgccagtg gcgctgggga ctggcctttc tgcgggaatg cctgcccgcg cgcacggccc
     3421 gcaatacggc cgcgatcgcc agcctcgcct gctacagccg tgaccagctc cgcgccctgc
     3481 gtgacgccac cgggctcgaa tatgcggcgc agacccaggg catcctgcat ctgttcacca
     3541 catcgcggga gcttgtccat gcccgtgacc acgagcgcat cctgtcgcgc ctgggcattc
     3601 gggcaaacct cctgacgtcc gcccaggcga tcagcctgga gccggccctc gcaccgctgg
     3661 ccgaccgact gaaaggcgcc ctgcacgggg tcgacgacga atccggtgat gcgcacctgt
     3721 tctgccgcgc cctggccgag cgcgccgccg ccgcaggggt gaccttcctg tacgacagcg
     3781 ccgtgtattc catggagacg ctcgcgggtc gcgtggccgg cgtgcgtgtc ctcgactcgg
     3841 cccgtcgcgc cggcgtgctg accgcggacg ccttcgtcgt cgcggcgggc agctacagtc
     3901 cggccctcct ggcgcgcgtg ggggaggccc tcaacattta cccggtgaag ggctactcgg
     3961 tcaccgcgca gatcgtggat cccgaacggg cgccgcacct gagcctcacc gacgagtccc
     4021 gccgtgtggt gtgctcacgc ttcggcgatc ggctgcgggt agccggcacc gcggagctga
     4081 acggcttcga cctgagcccc aacccggccc gcagcaatgc cctggtgcgc tggctggaag
     4141 aacattttcc cggtgtcacc catagccgca aggcggaaca ctggtgcggg ctcaggccgg
     4201 caacacccag caatgtgccc atcatcggac ggggccgtta cgacaacctg ttcctgaatg
     4261 ccgggcacgg caccctgggc tggaccctgg cctgcggatc ggcgcgggcg ctcgccgaca
     4321 tcatcacggg ccgcaagccc gaaccggagt ttccgttccg gggcctggcg cgctgactca
     4381 gccttcggtg gactcttcaa acagcacgcg gtaaacgtag gcggacagga cgacctgttc
     4441 ggcgtggggc aggctgtcga actgaagacc gtgattgacc atggacggct caccttcggt
     4501 cgccgccgtg gcgtgaaccg tacgcagcac tgcatcgagg gtcaggtact gctccacatc
     4561 actgaccacc acgcggaact tcaccttgat ccggtcatcc ttgagcccaa tcggggactt
     4621 ggccaccagc atggcaccac cgatgctcag atcgacgacc gtgcccgcat gggaacgccc
     4681 gtcagcaccg gataccgccg cgatgagatt gacccgcgcc cgggcccctc gacgcaccac
     4741 gagcccccgg acctggcttg gataggtgag gtggagatgg ggatagggta cgttcgcgac
     4801 cttgaagatg gtcgcgccaa aggcataaac gctcttgccc gagaacatgc gcaccacgaa
     4861 ggcctgtccc tcgcgcatca gcagcacctt gccgtcttga gtcggtgtgg tgacgacgac
     4921 gctcttgccc ttgaggtagc caatgagctt gacgtaatgg cggctctggg atgtctcgct
     4981 ctgggcctgc aactgcaggg tgtcgccgat atccatgcgg atctcgtcaa gcccgcgtag
     5041 atcctcggcc ggacgctccc cgccctcgcc cgtcggacgt tcatcctcga ccgtcttgcg
     5101 aaacacgccc tgtccgaaca gggccgcctt tcgctctgca ttctcgacca ccgccccgcg
     5161 ggcaagcagg ggctgcttct tgtcgtcgta gaggggccag cgcaggggct gaccgatttc
     5221 aatctcgtcg attccgacgc ggacgaacgt catgactgac tcgcttgctg aacacaacaa
     5281 cgagacctat cgtcaccgac cggaaaaacc ttagcgaagc ggaatacacg ccgcgccgtc
     5341 gatcaggcgg gcgagccttc caccggactg acaggggccg gcgtacaaca cgcgcccgcc
     5401 tcgaccggat tgccacatgc atccacctgg ggcggacggt ggatctcgcc cgtggcagtg
     5461 tcgtatccga tggattccag ataagccgcc agcgcagcgt ccatcgtcgc cacatgattg
     5521 tcgaaccaga tcgggagttc ctggatcagc tggcgtccga gcgcctcatc accccgctcc
     5581 atccgcttgc gcacatccgc cacgacggca agcacccggt cgtgttcggc cttgtgacac
     5641 gcggggaaat cgacctgctc catccagcgg ttttcctggt cgaagtgggc gaccgtatgc
     5701 gcaatgaagg cgtccagttc cgtcagcaag gcctcgccgg tcacctggag gaggcggttg
     5761 taatgatcga cgaactcctt gtgggtatcg tccatcggcc ccagacccag agccagtttg
     5821 tcagtccatt gcatggcggc catgtgatga attccgtagc gaaagaggac agcttagcgc
     5881 ccgccgtaca gacccaccct gaggcaagtc aagcggtgtc gcgccggctt atttgacgag
     5941 cttgaggcgc ccccgggcac cgcctttcgg cccatcgtcg ggcggcgtat cggcagttgc
     6001 aggcggcgac tcggaagccg tggcatcggg ctcggacgct tcagccggat cggacggatt
     6061 gacctcgaac gccatcccgt gaccgttctc gcgggcatag atcgccgaca cattgtctac
     6121 agggaccgcg atgtggtggg cgacgcctcc gaatcgggcc tggaaggcaa tcagttcatt
     6181 gcccatcacc agattctggg tcgcatccat gctcacattg aggacgatct gtccgtcctg
     6241 gacgaacgca cgcggcacct gcgtccgccc atcgacatgt acggacaggt agggcgtcag
     6301 cccctgatcg acgcaccact ggaagatggc gcggatcaga taaggcttgg tggaaacttc
     6361 actcatggga ctggatcctg caaaggcgca cgcccggagg acgcgcgcca gacccgcgct
     6421 cagcggcgca tgaccttttc cgacggggtc atcgcgtcaa tgaacccctg acggctgaag
     6481 atgcgctcgg cgtatttcat cagcggcgca gcggtacgcg gcagctcgat gccgtagtgg
     6541 tcaagacgcc acagcagcgg cgcgatggcc acgtccagca tggagaattc ctcgccgagg
     6601 aggaatttct gcttcgtgaa gatcggcgcc aactgggtca gctggtcgcg gatatgggcc
     6661 cgctccttgt ccgcctcctt ggcgttcttc tcgagggcct ccacatggga gaacagctcg
     6721 agttcgaagg tatgcaacag ctggcgggcc cgcgcacgca tgatgggatc gggcggcatc
     6781 agctggggat gcgggaaacg ctcgtcgatg tactcgttga tgatgttcga ttcgtacaga
     6841 atcaggtcac gatcaaccag cacgggaacc cggttgtacg gattgatgac ggcgatgtct
     6901 tccggcttgt tgaagagatc gacgtcaatc acctggaaat ccatgccctt ttcgaagagc
     6961 acgatgcgac agcggtgact gaacggatcc gtcgtcccgg aatacagatt catcatcgtt
     7021 gtgttacccc acggatactc agaatcacgc accacgcgac gggcgcgtgg tgcgtcttgc
     7081 tcaagaacag caagagactg catcggctga aagcagctgc cgattagtga acgtctttcc
     7141 agtaattctt cttgagaagg taggaaagca cgaagaggac gcccaggaag gcgaggacga
     7201 aggtgccgat ggtcttgcgg gtgcccgcca tgggctcacc catccacacg aggaaggaga
     7261 cgagatcggc ggtggccttg tcgtattcat ccttgctcat cttgcccggc ttgcccggtt
     7321 cgatgtgcac gaccttggtc atgttgccat gaccgtcatc gtgctcctcg aacttggcca
     7381 cctgatcgcc ctgcagttca tacaggacat gaggcatgcc aacgttttcg aacacggtgt
     7441 tgttccagcc ggtcggccgg cccggatcgc ggtagaagct gcgcagatag gtgtagagcc
     7501 agtctgcacc actgccgaac tcggaggcgc gggaacgggc gatcagggtc agatccggag
     7561 gcgcggcacc gaaccaggcc ttggcttcat cacgctgcat ggcgatgcgc atcggctcac
     7621 caatcttgtc ggcggtgaac atcagattgt ccttgatcat ctgatcggac agaccgattt
     7681 ccttcagccg gttgtagcgc agatagctcg caccgtgaca gttcaggcag taattgacga
     7741 agagcttggc gccgttctgg agcgcagccg ggtcagaaga caccggggcc ttgtccaggt
     7801 gcaactcggg gccgctcgcg accgccagca gcggcgcgaa cagggcagtg gcgaacagct
     7861 ttttcacgag actgagtccg cgggttttca tttccaggtc accctttccg gttcgggttt
     7921 ggtcttgtcg atcttggagt accacggcat cagcaggaag aaaccgaagt agatcaccga
     7981 gcagatttgc gacaccaggg tacggcccgg ggtcgggggc aggacgccaa ggtagccgag
     8041 gatgaagaag gccacgatga agaccgcgag gatggccttg aagaggccgc ccttgtagcg
     8101 gatggacttc accgggctgc ggtcaagcca gggcaggaag gcgatgatga tcaccgcggc
     8161 acccatggcc actacgcccc agaacttcgc gtcgataccg aacagcggat aggtcaccgc
     8221 gcggaggatc gagtagaacg gggtgaagta ccacaccggc gcaatgtgcg gcggcgtctt
     8281 cagcggatcg gcggggatga agttgttgaa ctccaggaag tagccgccgc cttcaggcgc
     8341 gaagaacacg atga
//
LOCUS       DAOQPA010000135         8247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4782_length_8247_cov_15.721313, whole genome shotgun sequence.
ACCESSION   DAOQPA010000135 DAOQPA010000000
VERSION     DAOQPA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_8247_cov_15.721313"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1821)
                     /gene="mutL"
                     /locus_tag="PKX01_14760"
     CDS             complement(1..1821)
                     /gene="mutL"
                     /locus_tag="PKX01_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMZ77442.1"
                     /translation="MPIQVLPDHLINQIAAGEVVERPASVLKEVLENALDAGATALDV
                     QLDQGGVRRIRVADDGGGIPKDELPLALERHATSKIASLDDLERVGTMGFRGEALAAI
                     AAVSRLSVTSRADGAPHAWRIDAHPRELAPAALNHGTVVDVADLYFNTPARRKFLKSE
                     GTEFAHCDDVFRRVALARPDVALQLTHNGRVGLRLPAGPREARVRALLGDDFLAAARA
                     VEAASGLLRLHGYASLPAYARSNRDAQFFYVNGRFVRDKLIAHAIREAYADILHGSRH
                     PAFVLFLELDPFGVDVNVHPAKTEVRFRDARAVHQFVFHALTRALSASGAAQTGEPDR
                     DGAGITPRPVPSHAGGAPYAPAPQQGRLAMEPVSRAYFDFAAAARPAGPAPVGASPGV
                     SAGAVGNGPETPHAPLPASDLAPPLGYALAQLHGVYILAQNEAGLILVDMHAAHERIL
                     YERLKTLLDGTPGVQRLLIPAVFGVGAQDMASAEEHAELLARMGFEIGPAGPQELAVR
                     SIPALLAAAPVGELVRALLAELRDFPATEVVTARRNELLATMACHGAVRAHRSLTLPE
                     MNALLREMEATERADQCNHGRPTWTSLSMAELDRLFLRGR"
     gene            1926..2777
                     /locus_tag="PKX01_14765"
     CDS             1926..2777
                     /locus_tag="PKX01_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77443.1"
                     /translation="MQLTYIRPQGRLAEVVAGYLFASTLDESWPTDMPWLSSIPAGNE
                     WALTVFTEGRAVLLGDGLAPPQVLPSWVLSGPRLRPVTLMNLCPLRAVSVLFHGDALA
                     RLVNLAPVHLPQGCVDPAELLGADWALFRSALGARAGDRVAQQNWLEAFLEPRWEARR
                     LGLSRAPTATVRAAGRIAAAASARAMGWHERCIERSGPRAIAVTPAVGRHVALIHGAL
                     MEAWGLGPAAGARRLGDMARALGVAHPAGLVRALAARTWVGSERVTSAETASPPEALR
                     SPVRPAG"
     gene            complement(2749..3960)
                     /locus_tag="PKX01_14770"
     CDS             complement(2749..3960)
                     /locus_tag="PKX01_14770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMZ77444.1"
                     /translation="MRLSPLASLASLLTGALAVVATSTAHADTPKRYIVQFQDTAVQA
                     PPRGPVHDRRADEDPRGWGYVDSRVIERVQSTESRHGIQALHAYSSVIKGFAAALSDA
                     QVARLKADPTVLSIEEDLPMHTTAQTIPWGISTTRATLSSTLAGDGSGTVSTVNVYVI
                     DTGIAPHGELNKINHVNFTGDGKNTDCNGHGTHVSGTIGARDNTADVVGMAPAVALTG
                     VKVLDCTGSGSTSTVIKGIDWVTANAVKPAVANMSLGGSASTTLDAAVTKAVNAGIVF
                     AIAAGNSAANACNYSPARVGGSLAGAITTAATDSANKEASFSNYGSCVDTWAPGVSIL
                     STYLNAGMATMSGTSMATPHVTGAAALYLSRNPNATPAAVEAAIKQAAVAPGTVSKDG
                     RAITRLDVLGF"
     gene            4068..4922
                     /locus_tag="PKX01_14775"
     CDS             4068..4922
                     /locus_tag="PKX01_14775"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HMZ77445.1"
                     /translation="MPPYPRSVAPAGRAVLDHALVILRPLVAWLLRSGVGYGEFAAAL
                     KPVFLDMALAEVARGGTKLTDSALSLLSGLHRKDVRALVPVLQGAADPSAALGKPSPA
                     SQVATRWLTNGWPERLPVAGPEPSFEALARSVSQDFHPRAVLLELIRLGVVEEADGAV
                     HLRQAAFVPDARHEETRRLMAGSVADHLAAGVHNLTEDGSRRFLEQSVFADGLSAESA
                     RALEQLANALWQDVLTAMVQAAVPLCEKDEAAGGDQRIRLGMFCYSAPMAGAAAPDAA
                     ADEKDPPV"
     gene            4919..6409
                     /locus_tag="PKX01_14780"
     CDS             4919..6409
                     /locus_tag="PKX01_14780"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HMZ77446.1"
                     /translation="MKTRFLAPVSGRLARWAARPRLSAAMFLIALLSACGGGGAFDVA
                     GVGSGGSGVASGTVTGFGSVIVDGVEYDDSSASRQSEDATGSLANAPVKLGQQVQLNY
                     SGSRVAQRIEVIAQLLGPVTAAPGSDGRLAVMGQPVRVVTSVSDASQSTPTVLDGYAA
                     VTAIAVGDEVEVHGAWVYDSAVASNVLVATRVEKHSVAADPVQLGGVVTAVGSGTLRL
                     NSATGTQVSGSDLAEIAVGQVVRVWAARSALAASPVVARRVAVQGVTASELGTAGGTL
                     SGLASQYDPASRSVSIQGVRVKLPDSMTVDSAALARGEFVTVQVTASGSTLVASTVAQ
                     RSGSAGADLGRTVEVKGVTRGIDWSATAVSFTLRDTPIVAAAAAIDASCRSVSPTASV
                     YVEVRGALAQSAATVTANSVKCSQSVPGSATVDYTGTLAAIDASARTLTLRVDVSGAS
                     VSGTWDAQTYFEQRPASLPVGLRVEVEGVMDQGSSTLRLSKVRLED"
     gene            6473..8065
                     /locus_tag="PKX01_14785"
     CDS             6473..8065
                     /locus_tag="PKX01_14785"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HMZ77447.1"
                     /translation="MNLFFNLPRRERVLGVVPVALALALAGCGGGGGSTSSASASASS
                     SSTATESSSGTVTGFGSVFVDGVELEDAKAVTRVENADGSYSLTSTQLGQRVRVAHDA
                     TGTASTVTVDAAVIGTVASGSVDATALTFTVAGQSVKANTDSTSGPLTVYGGGYTAFS
                     DIAAGDLVEIHGSAVYDSTAKVYVVQATRIEKKSAISAVRVMGKIASLDTTAKTFAIN
                     SLTVAYGSAILAPSTATLADGVTVVAWGAQNSLVSGSSGLSLTASRVRVLNAGLADDS
                     ASGATQLGGVVSNYSVSTGTFELEGVKVIVGSATLSPTGATIANGAYVQVSGTVGSDA
                     SITATSIKVRQQSTTDDTASVRLIGAIESLSDQTSFVVRGVPVDASAITLSTACPGVT
                     LAVGTVVKVTATVQSGTDVVKATSLGCTSATTYTMLSASGTAGTVNTTVRSFVLTTTG
                     TATTTRTVQWSDQTVFAGVTAATLDGVTVAVEGYLDSSGVLVARKIRNPAVAAGREDA
                     DAYAGVDDGSGRSWSRYRSTHR"
BASE COUNT         1146 a         2495 c         3297 g         1309 t
ORIGIN      
        1 tcaccgtccc cgcaggaaca gccgatccag ttccgccatg ctcagggacg tccaggtggg
       61 ccggccatgg ttgcactggt cggcccgttc ggtggcttcc atttcgcgca gcagggcgtt
      121 catctcgggc agggtgaggc tgcggtgggc gcggacggcg ccgtggcagg ccatggtggc
      181 gaggagttcg ttgcggcggg cagtgacgac ttcggtggcg gggaagtcgc gcagttcggc
      241 gaggagggcg cggacgagtt cgccgacggg ggccgcggcg aggagggccg ggatgctgcg
      301 cacggcgagt tcctggggac cggcggggcc gatttcgaag cccatgcggg ccaggagctc
      361 ggcgtgttcc tcggcgctgg ccatgtcctg ggcgccgacg ccgaagactg cggggatgag
      421 caggcgctgg acgccggggg tgccgtcgag gagggtcttg aggcgttcgt agaggatgcg
      481 ctcgtgggcg gcgtgcatat ccacgaggat caggccggct tcgttctggg cgaggatgta
      541 gacgccgtgg agctgggcga gggcgtagcc gaggggtggg gcgaggtcgc tggcggggag
      601 gggagcgtgc ggggtctccg gaccgttgcc aacggcgccg gcagaaacgc cgggtgacgc
      661 gccgacgggt gcagggcccg ccggccgggc tgcggcggcg aagtcgaaat aggcgcggga
      721 gaccggctcc atggcgaggc gaccttgctg gggtgccggc gcatagggtg cgccgcctgc
      781 gtggctgggg accgggcggg gcgtgattcc cgcaccgtcg cggtcgggtt cacccgtctg
      841 ggcggcgccg gaggcggaga gggcgcgggt gagggcgtgg aagacgaact ggtgcacggc
      901 gcgggcgtcg cggaagcgca cttcggtctt ggcgggatgg acgttcacgt ccacgccgaa
      961 ggggtcgagt tccaggaaca gcacgaaggc cgggtggcgg ctgccgtgga ggatgtcggc
     1021 gtaggcctcg cggatggcgt gggcgatcag cttgtcgcgc acgaagcgac cgttcacgta
     1081 gaagaactgg gcgtcgcggt tgctgcgggc gtaggcgggc agggaggcgt agccgtgcag
     1141 gcggagcagc ccggaggcgg cttccacggc acgggctgcg gccaggaagt cgtcgccgag
     1201 gagggcgcgg acgcgggcct cacgcgggcc tgcgggcaga cgcagaccga cgcggccgtt
     1261 gtgggtgagc tgcagggcca cgtccgggcg ggccagggcg acgcggcgga agacgtcgtc
     1321 gcagtgggcg aactcggtgc cttcgctctt gaggaatttg cgccgggcgg gggtgttgaa
     1381 gtagaggtcg gcgacatcca cgacggtgcc gtggttgagg gcggcagggg cgagttcgcg
     1441 cgggtgggcg tcgatgcgcc aggcgtgggg ggcgccgtcg gcgcggctgg tgacgctcag
     1501 gcgcgagacg gcggcgatgg cggccagggc ttcgccgcgg aagcccatgg tgccgacccg
     1561 ctccagatcg tccaggctgg cgatcttgct ggtggcgtgg cgctccaggg cgaggggcag
     1621 ttcgtccttg gggatgccgc cgccgtcgtc ggcgacgcgg atgcggcgta cgccgccctg
     1681 gtcgagttgc acgtcgaggg cggtggcacc ggcgtcgagg gcgttctcga ggacttcctt
     1741 gaggacggag gcggggcgtt ccaccacttc gccggcggcg atctggttga tgagatggtc
     1801 gggcagaacc tggatgggca tcgggcgtcc ggtgaaacga tggaggggcg gggcgcattg
     1861 taatggctcg cgacgggccc gtccggggca taatccggcc tgcatccggt ttgtgacatt
     1921 cgatcatgca actgacctac atccgccctc aggggcggct cgccgaggtg gtggcgggct
     1981 acctcttcgc ttcgacgctg gacgagtcgt ggccgaccga catgccttgg ctgtcctcca
     2041 ttccagcagg taacgaatgg gcgttgacgg tgttcaccga gggacgtgcg gtgctgttgg
     2101 gggacggtct ggcgccgccg caggtgctgc cgtcgtgggt gctcagcgga ccgcggttgc
     2161 gccccgtcac gctgatgaac ctgtgcccgt tacgggcggt gtcggtgctc ttccacgggg
     2221 atgccctggc gcggctggtg aacctggcgc cggtgcatct gccgcagggg tgcgtcgatc
     2281 cggcggagct gctgggcgcg gactgggcct tgttccgctc ggccctgggc gctcgcgcgg
     2341 gagaccgggt ggcccagcag aactggctgg aggcttttct cgagccccgc tgggaggcgc
     2401 gccgtctggg gttgtcccgt gcgccgacgg cgaccgtgcg ggcggccgga cggatcgccg
     2461 cagcggcctc ggcgcgggcg atggggtggc acgagcgctg catcgagcgg agcggtccac
     2521 gggcgatcgc ggtcactccg gccgtgggcc ggcacgtggc gctcatccac ggggcgctga
     2581 tggaagcctg gggattggga ccggcggcgg gggcccggcg cctgggtgac atggcgcggg
     2641 cgctgggggt ggcgcatccc gctggcctgg tccgggcgtt ggcggcccgg acctgggtgg
     2701 gctcggagcg ggtgacgtcg gcggagacgg cgtctccgcc cgaagcactc agaagcccag
     2761 tacgtccagc cgggtgatgg cccggccgtc cttgctgacg gtgccgggcg cgacggccgc
     2821 ctgcttgatg gcggcctcca cggcggccgg cgtggcgttt ggattgcggg acaggtagag
     2881 ggccgcggca ccggtcacgt ggggcgtggc catggaggtg ccggacatgg tcgccatgcc
     2941 ggcgttgagg taggtggaga ggatggagac gcctggcgcc caggtgtcca cgcagctgcc
     3001 gtagttgctg aaggaggctt ccttgttggc gctgtcggtg gcggcggtgg tgatggcgcc
     3061 ggccaggctg ccacccacgc gggccggcga atagttgcag gcgttggcgg cgctgttgcc
     3121 ggccgcaatc gcgaagacga tgcccgcgtt caccgccttg gtgacggccg catccagcgt
     3181 ggtgctggcg ctgccgccca ggctcatgtt ggccaccgcc ggcttgacgg cgttggcggt
     3241 gacccagtca atgcccttga tcacggtgga ggtgctgccg gaacccgtgc agtcgagcac
     3301 cttgacgccg gtgagcgcca ccgcgggcgc catgcccacc acgtcggcgg tgttgtcgcg
     3361 ggcgccgatg gtgcccgaca cgtgggtgcc gtggccgttg cagtcggtgt tcttgccgtc
     3421 gccggtgaag ttcacatggt tgatcttgtt caactcgcca tggggtgcga tccccgtgtc
     3481 gatcacgtac acattgacgg tgctgacggt gcccgagccg tctcccgcca gggtggagga
     3541 cagggtggcg cgggtggtgg agatgcccca cggaatggtc tgggccgtcg tatgcatggg
     3601 caggtcctct tcaatgctga ggacggtcgg gtcggccttg aggcgggcca cctgggcatc
     3661 gctgagggcg gccgcgaagc ccttgatgac gctgctgtag gcgtggagag cctggatgcc
     3721 gtggcggctt tcggtgctct gcacgcgctc gatcacgcgg ctatccacgt agccccagcc
     3781 gcggggatct tcgtcggcgc gtcggtcgtg gaccggaccg cggggtgggg cctgcacggc
     3841 ggtgtcctgg aactggacga tgtagcgctt gggggtgtcg gcgtgggccg tggaagtggc
     3901 gacgacggcg agcgcgccgg ttagtagtga cgcgagtgac gcgagtgggg agaggcgcat
     3961 ggtggactcc atggtcggga ccggtgcgga cgcggcggcc ggtccggggt gtaagggaaa
     4021 gttgactata catgggaaaa aatcccatat atagtctccc tatggaaatg cctccctacc
     4081 caaggtcggt ggcgcccgcc gggcgggcgg tgctggatca cgccctggtc atcctgcgtc
     4141 cgctggtggc gtggctgctg cgctcgggcg tgggctacgg ggaattcgca gcagcgctca
     4201 agccggtgtt cctggacatg gccctggccg aggtggcgcg gggcggcacc aagctcacgg
     4261 attcggccct cagcctgttg tccggtcttc atcgcaaaga cgtgcgggcc ctcgtgccgg
     4321 tgctgcaggg cgccgccgac ccgtccgcgg ccctcggcaa accgtcgcct gccagtcagg
     4381 tggccacgcg ctggctcacc aacggctggc cggagcgcct gccggtggcg ggtccggagc
     4441 cgtccttcga agcgctggcg cgttcggtgt cccaggattt ccaccctcgc gcggtgctgc
     4501 tggagctgat ccgcctgggg gtggtcgagg aggcggacgg ggcggtgcat ctgcgtcagg
     4561 cggctttcgt gcccgatgcc cgccacgagg agacgcggcg cctgatggct ggcagcgtgg
     4621 cggatcacct ggcggccggc gtgcataacc tcaccgagga cggttcgcgc cgcttcctgg
     4681 agcagagcgt ctttgccgac gggctgtcgg cggagtcggc gcgggccctg gagcagcttg
     4741 ccaacgccct gtggcaggac gtgctgacgg ccatggtgca ggccgccgtg cccctgtgcg
     4801 agaaggatga agcggcgggg ggcgatcagc gcatccgcct gggcatgttc tgttattcgg
     4861 ccccgatggc cggcgctgcc gcgcccgacg cggcggcgga cgaaaaggat cctcccgtat
     4921 gaagacccga tttctagcgc cggtgtcggg gcgtctggcc cgttgggccg cccgcccgcg
     4981 cctgtctgcc gcgatgtttc tcatcgcatt gctgagtgcc tgtggcggcg gcggggcatt
     5041 cgatgtggcc ggtgtgggca gcggcggcag cggggtggct tccgggacgg tgacgggctt
     5101 cggctcggtg atcgtggacg gtgtggaata cgacgacagc agcgccagcc ggcaaagcga
     5161 ggacgcgacg ggcagtctgg ccaatgcgcc ggtcaagctc ggccagcagg ttcagctgaa
     5221 ctattccggc agtcgggtgg ctcagcgcat cgaggtgatc gcccagttgc tggggccggt
     5281 gacggccgcg ccgggcagcg acgggcggct tgcggtgatg gggcagccgg tgcgcgtggt
     5341 gacgagcgtc agcgatgcca gccagagcac gccgacggtg ctcgacggct atgcggcggt
     5401 gacggcgatc gcagtgggcg acgaggtgga agtgcatggc gcctgggtgt acgacagtgc
     5461 cgtggcgagc aatgtgctgg tggcgacacg cgtcgagaag cacagcgtcg cggcggatcc
     5521 ggtgcaactg ggcggggtgg tgacggccgt aggcagcggg acgctgcgcc tcaactcggc
     5581 gaccggcacc caggtgagcg ggtcggatct ggcggagatc gcggtgggcc aggtggtgcg
     5641 ggtgtgggcg gcgcgcagtg cgctggcggc gtcgccggtg gtggcacggc gtgttgcggt
     5701 gcagggtgtt acggcgtcgg agctgggcac ggccggcgga accctgagcg gcctcgcttc
     5761 ccagtacgat ccggccagcc gcagcgtgag catccagggc gtgcgcgtga agctgccgga
     5821 cagcatgacg gtggacagtg ccgccctggc ccgtggtgag ttcgtgaccg ttcaggtgac
     5881 ggcgtcgggt tcgacgctgg tggcaagcac cgtggcccag cgcagcggat cggctggggc
     5941 ggatctgggc cgcaccgtgg aggtgaaggg tgtcacccgc ggcatcgact ggagcgcgac
     6001 ggcggtgagc ttcaccctgc gggacacgcc catcgtggcc gccgccgccg cgatcgacgc
     6061 gtcctgccgg tcggtgagtc cgacggccag cgtctatgtg gaggtgaggg gagccctggc
     6121 ccagtcggcg gcgacggtga cggcgaattc cgtgaagtgc agccagagcg tgcccggttc
     6181 ggcgacggtc gattacaccg ggacgcttgc ggccatcgac gcaagcgccc ggaccctcac
     6241 gctgcgcgtc gatgtttcag gtgccagcgt gagcgggacc tgggacgccc aaacctattt
     6301 cgaacaacgc cccgccagtc tgccggtcgg ccttcgggtc gaggttgagg gtgtgatgga
     6361 tcaaggcagc agcacgttgc gactcagcaa ggtccgcctg gaggattgaa gcgccgggcg
     6421 ggtcggccca tttccgacat gaaccgacat tgaccgacat gaggagtcga gcatgaatct
     6481 attctttaac ctgccccgcc gggagcgggt cctgggcgtc gtgcccgtgg cgctggccct
     6541 ggcgctggcg ggctgcggcg gtggcggcgg cagcacgtcg tccgcgtccg cgtccgcgtc
     6601 atccagcagc accgccaccg aatcttccag tggcaccgtg acgggcttcg gcagcgtgtt
     6661 cgtcgacggc gtcgagctgg aggacgccaa agccgtgacc cgcgtggaga acgcggatgg
     6721 cagctacagc ctgaccagca cgcaactggg gcagcgcgtg cgcgtggccc acgatgccac
     6781 cggtacggcg tccaccgtca ctgtggatgc cgccgtgatc ggcacggtag cgagtgggag
     6841 cgtcgacgcg acggcgctca cctttacggt ggccggccag tcggtcaagg ccaataccga
     6901 cagcacgtcc ggcccgctga cggtgtatgg cggcggctac acggcctttt ccgacattgc
     6961 cgccggggac ctggtggaaa tccatggcag cgcggtgtac gacagcacgg ccaaggtgta
     7021 cgtggtacag gccacccgca tcgagaagaa atccgccatc tcggcggtgc gcgtgatggg
     7081 caagatcgcc agcctggaca ccacggcgaa gacctttgcc atcaacagcc tgacggtggc
     7141 ctatggctcc gccatcctgg cgccctccac cgccaccctt gcggatggcg tcaccgtggt
     7201 ggcctggggc gcccagaact ccctggtgag cggcagttcg gggctgagcc tgaccgcctc
     7261 gcgggtgcgc gtgctcaatg ccggtctggc tgacgacagc gccagtggcg cgacccagct
     7321 cggcggcgta gtgagcaact acagcgtcag caccggaacc ttcgaactgg aaggcgtgaa
     7381 ggtgatcgtc ggcagcgcga ccctcagccc gaccggcgcg acgatcgcca acggtgccta
     7441 cgtgcaggtg agcgggaccg tcggcagtga cgcgtccatc accgcgacga gcatcaaggt
     7501 gcgccagcag agcaccaccg acgacaccgc cagcgtgcgc ctgatcggcg ccatcgaaag
     7561 cctgtcggat cagacgtcct tcgtggtgcg cggcgtgccg gtggacgcct cggcgatcac
     7621 cctgagcacg gcctgcccgg gcgtgaccct ggcggtgggc acggtggtga aggtgacggc
     7681 gacggtgcag agcggcaccg atgtggtcaa ggccaccagc ctggggtgca cctcggcgac
     7741 gacctatacg atgctgagcg ccagcggcac ggcgggcacg gtgaatacga cggtcaggtc
     7801 cttcgtgctc accacgaccg gcaccgccac cacgacccgt acggtgcagt ggagcgacca
     7861 gacggtgttt gcgggcgtca cggccgcgac gctggacggc gttacggtgg ccgtggaggg
     7921 ctatctggac agcagcggcg tgctcgtcgc ccgcaagatc cgcaatcccg ccgtggctgc
     7981 aggccgtgag gacgctgatg cctatgcggg cgtggatgac ggctcggggc gttcgtggag
     8041 ccgctatcgc agcacgcaca ggtgatctga accgcgtgga ccggtccgca ggagaccggc
     8101 gattcgcgcg accgctgtcg caacttggcc gggcacgtgt gcccggtttt tttgcgtggc
     8161 gggagctgcg gcgatcagcg ttcccgcagg gattcgtcga gggtgcggat cagcggatag
     8221 aggaagaagg cgatcaccga gcgtcgg
//
LOCUS       DAOQPA010000136         8176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4855_length_8176_cov_18.092353, whole genome shotgun sequence.
ACCESSION   DAOQPA010000136 DAOQPA010000000
VERSION     DAOQPA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_8176_cov_18.092353"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKX01_14790"
     CDS             <1..951
                     /locus_tag="PKX01_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77448.1"
                     /translation="AAVLGRTARRALRKGLSRDQWGIALAFGDDVCLDPARAQLILPP
                     PDRFWADPMVWPAGQDGWWVFIEEVLYAEGRGTLQALRVHRDGRWEAPVPVMARPWHL
                     SYPFLFWWDGALWMVPESGANRRVELYRCTRLPDRWEKFTDLLTDIDLVDATLFAHDG
                     RWWLLGNVSEPGADKHDELHAFHAPSPLGPWTPHAANPVVADARVARPAGPVFERDGH
                     LLRPAQVCVPEYGHALVLRRIEKLTTEEYVERTERRIDPGWLPGIACVHTLSHEAGLT
                     AFDFLIRRSRLLPPPAALRRAVLHGGGEERCDMPRAEVPR"
     gene            948..1679
                     /locus_tag="PKX01_14795"
     CDS             948..1679
                     /locus_tag="PKX01_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HMZ77449.1"
                     /translation="MRIQMMGCWVDNLTMEETLGTVENFIHSGRPHQHVVVNVDKIVK
                     ASRDPVLRNIINGCDLINADGMPVVWASRLLGKPLKERVTGVDLFESLMERSAEKGWR
                     VFLLGAREEVVSRVAQIYPARYPGLTIAGYRNGYWKPEDEAGVANAIAATRPDILFVA
                     ISSPKKENFLARYQAAMKIPFAMGVGGTFDVAVGLVRRAPVWMQKAGLEWFYRFLQEP
                     RRMFRRYFIDDMAFVALFAREWMKR"
     gene            1696..3129
                     /locus_tag="PKX01_14800"
     CDS             1696..3129
                     /locus_tag="PKX01_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77450.1"
                     /translation="MGLEDWGYRWLKRYMDAPQWIKEPLGRVYGLLPDSIRHGKDFGR
                     YRDEAGLTDAEALRRLRDARLRETLLWAAQTVPAYAAIAAQLLTDLPAEEWLSLFPLM
                     TKLDYRRNPEHYLSTGCSPAERLPMQTSGSVAEPFRFYLHKGVSRAKETAYIESYGRR
                     IGLTRKDVVLSLRGRNVRGSEADGGPLWSYEPIKRMLWISPNHLDGSYMPRHVQAAIE
                     HKVSFIQGYSSAVYELARWLDFNPVPRFSDAIRGVQLFSECAEPDMIALIEKVFGCPV
                     LIHYGHSERSVMAGSMPDDHRYFVWPLYGHIELIDEAGRPITEPGVQGEVVATGFDNR
                     VMPFIRYRTGDLAAWSDGPAHPDLPGYPVLERIDGRNNDFFVGRDGRRIPVASLGGLR
                     PPEFLQVEASQYEQFEPGRLIVRLQSPSPLPADTVAVLDNYFRVKLLGCVEPSLEVVP
                     HIARTARGKRRLMIQHVTSEVEEGSRV"
     gene            3122..4213
                     /locus_tag="PKX01_14805"
     CDS             3122..4213
                     /locus_tag="PKX01_14805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ77451.1"
                     /translation="MSKKAPALIMLGPDPEARGGIASVQRIWLQAGLLDERPVRVLTT
                     YRDGSALRKLGAFVTALLRFLLLLAAGRVAAVHVHSASNASFRRKSVFLALARLAGKP
                     YVFHLHGGAFDHYYAGASFLERAWVDRTLHGAAVVFTLSEGWANWLRINVGHPDVRVL
                     PNPVLPVTLPAGVAREPATLLFLGRLEDAKGVFVLVKAIARLARKFPDLRAVIAGDGD
                     IRAVRDAAERAGVLDRIETPGWVDGGKKLRLLARAGVFVLPSRFEGLPMSVLEALAAG
                     LPVVASRVGGIPQVVRNGDNGALHEADDVASLASALERVLADPAAARAMGERGRRRVL
                     ADYGVERIRALLNAVWDSLMTPASERFAR"
     gene            4210..5604
                     /locus_tag="PKX01_14810"
     CDS             4210..5604
                     /locus_tag="PKX01_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HMZ77452.1"
                     /translation="MIVSCRSPLYQLAPVALQERLLSWQTRLRRWLTRPAALAQTRHA
                     IADSQWLSREGWQSLQFAAVRDLALHAARHTRYYQDVFACLGTDPSAWTSLDDLSRVP
                     ALSKDEVISIGDGLLARDGTWLRFQGSTSGTTGRAMRGWRDQASVTFEQAFVDRQAEW
                     AGYRPGDRRAWLRGDPIVPTRQQGGPLWRFNRADNMLMLSSYHLSRYNGLAYLHALES
                     FDPVLLQAYPSSVAYLARLLEDEGRHYRGPALRGIVTSSETLGADDRRVIAERFGCPV
                     FDWYGAFERVAAIGTCEHGRYHVMEDYGVTEFEDNGDGTANLIGTGFGNRSMPLLRYR
                     SDDRVVLADPDFQCGCGRSFRVVERILGRVDDVIRTPDGRHVVMLDWIFAGLFGLIEA
                     QVVQERLDLVRIRIVVAPEFSAQDEQHLLQRARERLGPQVTLRIERVPAIERTRNGKF
                     RQIVSRIHSRQDIR"
     gene            5601..7586
                     /locus_tag="PKX01_14815"
     CDS             5601..7586
                     /locus_tag="PKX01_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HMZ77453.1"
                     /translation="MSKLTRVGWYWNRLRCMSVAEMVERASTSAGHALERRRRPVPIP
                     PPDLTRRGACWLPAHELPPVPPAVIDRADEILGGRWRIFARGPVALGLPPEWNRNPHN
                     GAVLPMVFGKTMAFRDTTVIGDIKYLWEPNRHLELVVLAQAWKATGLRKYLDALGTLL
                     TSWFRQCPYPLGPNWSSALEAGIRLINWSIVWQLIGGFESPLFAPPEGVVLLRDWLDS
                     IWRHAEFIRGHRSLHSSANNHLIGELSGLYVASVTWPCWEDAPRWREAARAGLETEVL
                     RQNAPDGVNLEQATSYQQFVWDFLLFAGLAARATGDDFSTAYWQRMEAMLDYVAAIMD
                     TGGQVPQFGDADDGLACGLSLCADRDPFRSQLATGAVLFKRSAFARKVGRFDLRSVWL
                     LGRAAADDFATLLVDRSEPLTPRQAFPDGGVYVLGSALDTPREVRVVADAGPLGLGGI
                     AAHGHADALSFTLSMDGREFLVDPGTGTYHGPRAWRDGFRSTALHNTLTVDGEDQAIT
                     GGKFMWIRKYEVSVLEWSSSADADRLVAEHDGYRRLAGHPVHRRAWRLDKRSGTLSVR
                     DELGAPAPRPVALNWHFAENCVITCTPDGLVVGHDGVTLLVTLPPGGETTVICGSDVP
                     LCGWVSRGYDLRRASTTVIWRGHLGPGQAAETVFRRG"
     gene            7737..8123
                     /locus_tag="PKX01_14820"
     CDS             7737..8123
                     /locus_tag="PKX01_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HMZ77454.1"
                     /translation="MGLTPIRLLLFAMIVLALQYGWKHMPHPGAGGSPTAEALRSIAS
                     TVQPGEVTMYSTTECAYCAQARAWLTQYGFAFTECNMSLDRRCEREFLAYGATGTPYL
                     VIRRGGRTYHMKDGFDSEEFLRVLKG"
BASE COUNT         1154 a         3124 c         2635 g         1263 t
ORIGIN      
        1 gccgcggtgc tcgggcgtac ggcccggcgg gccctgcgca aggggctcag ccgcgaccag
       61 tggggtatcg ccctggcctt cggcgacgac gtgtgcctcg atcccgcccg cgcccagctg
      121 atcctgccgc cgcccgaccg cttctgggcc gaccccatgg tgtggcccgc cgggcaggac
      181 ggctggtggg tgttcatcga agaggtgctg tacgccgagg ggcgcggcac cctgcaggct
      241 ctgcgcgtcc atcgggacgg ccgctgggag gcgccggtgc cggtgatggc gcgcccctgg
      301 cacctgtcct accccttcct gttctggtgg gatggcgccc tgtggatggt gcccgagagc
      361 ggcgcgaacc ggcgtgtcga gctgtaccgc tgcacgcgcc tgcccgaccg ctgggaaaaa
      421 ttcaccgacc tgctcaccga catcgacctg gtggacgcca ccctctttgc ccacgacggc
      481 cgctggtggc tgctgggcaa cgtgagcgag ccgggcgccg acaagcacga cgagctgcat
      541 gccttccatg ccccgtcccc cctcgggccg tggacgcccc acgccgccaa cccggtggtg
      601 gccgatgccc gcgtggcgcg gccggccggt ccggtgttcg agcgggacgg ccatctgctg
      661 cggcctgccc aggtctgcgt gcccgaatac gggcacgccc tggtcctgcg ccgcatcgag
      721 aaactgacaa ccgaagagta cgtagaacgt accgaacggc gcatcgatcc gggctggctg
      781 cccggcatcg cctgcgtcca taccctgagt cacgaggccg gactgacggc cttcgatttc
      841 ctgattcggc gttcccgcct gcttccgccg cccgccgcac tgcgtcgtgc cgtgctgcac
      901 ggcggcggag aggaacgctg cgacatgccc cgcgcggagg tgccacgatg agaatacaaa
      961 tgatggggtg ttgggtggac aacctgacca tggaggagac cctcgggacg gtggagaact
     1021 tcatccattc cggccgaccg caccagcacg tggtggtgaa cgtggacaag atcgtcaagg
     1081 ccagccggga ccccgtgctg cgcaacatca tcaacggctg cgacctgatc aacgccgacg
     1141 ggatgccggt cgtgtgggcc tcgcgcctgc tcggcaagcc cctcaaggaa cgggtcaccg
     1201 gcgtggacct cttcgaatcc ctgatggagc ggtcggccga gaagggctgg cgcgtgttcc
     1261 tgctgggggc ccgcgaggag gtggtgagcc gcgtggcgca gatctacccg gcccgctacc
     1321 ccggcctgac catcgccggc taccgcaacg gctactggaa gcccgaggac gaagccggtg
     1381 tggccaacgc catcgccgcc acgcggcccg acatcctctt cgtggccatc agctcgccca
     1441 agaaggagaa cttcctggcc cgctatcagg ccgccatgaa gatccccttc gccatgggcg
     1501 tgggcggcac tttcgacgtg gccgtgggcc tcgtgcggcg tgccccggtc tggatgcaga
     1561 aggccgggct ggagtggttc taccgattcc tgcaggagcc gcggcgcatg ttccgccgct
     1621 acttcatcga cgacatggcg ttcgtggcgc tgtttgcccg cgaatggatg aaacgctgac
     1681 cgacggaacg agcacatggg actggaagac tggggttacc gctggctgaa gcgctacatg
     1741 gacgcccccc aatggatcaa ggaacccctg gggcgcgtgt atgggctgct gccggacagc
     1801 atccgccacg gcaaggactt cggccgctac cgggacgagg cggggctgac cgacgccgag
     1861 gcgctgcgcc gcctgcgcga tgcgcgcctg cgcgaaaccc tgctgtgggc cgcccagacc
     1921 gtgccggcct atgccgccat cgccgcgcag ctcctcaccg acctgccggc agaagagtgg
     1981 ctgtccctgt tcccgctgat gaccaagctg gactaccggc gcaacccgga gcactacctc
     2041 tccaccggct gctccccggc cgagcggctg cccatgcaga ccagcggctc ggtggccgaa
     2101 ccgttccgct tctacctgca caagggcgtc tcccgcgcca aggaaacggc ctacatcgaa
     2161 tcctacggcc ggcgcatcgg cctcacccgc aaggacgtgg tgctgtccct gcgcgggcgc
     2221 aacgtgcgcg gctccgaggc cgacggcggg cccctgtgga gctacgaacc catcaagcgc
     2281 atgctgtgga tcagccccaa ccacctggac ggcagctaca tgccgcgcca cgtgcaggcc
     2341 gccatcgagc acaaggtgag cttcatccag ggctattcct ccgcggtgta tgaactggcc
     2401 cgctggctgg acttcaaccc ggtgccgcgc ttctccgacg ccatccgcgg cgtgcagctc
     2461 ttctccgagt gcgccgagcc cgacatgatc gccctcatcg agaaggtctt cggctgcccg
     2521 gtgctgatcc actacggcca ctccgaacgc tccgtgatgg ccggctccat gcccgacgac
     2581 caccgctact tcgtgtggcc cctgtacggc cacatcgaac tcatcgacga agccggccgg
     2641 cccatcaccg aaccgggcgt gcagggcgag gtggtggcca ccggcttcga caaccgggtc
     2701 atgcccttca tccgctaccg caccggcgac ctggccgcct ggtccgacgg cccggcccac
     2761 ccggacctgc ccggctatcc cgtgctggag cgcatcgacg ggcgcaacaa cgacttcttc
     2821 gtcggccgcg acggccggcg catcccggtg gcctccctgg ggggcctgcg tccgcccgag
     2881 ttcctgcagg tggaagccag ccagtacgaa cagttcgagc cgggcaggct catcgtgcgc
     2941 ctgcagtcgc cctcgccgct gccggccgac accgtggcgg tgctcgacaa ctacttccgc
     3001 gtcaagctcc tcggctgcgt cgagccgagc ctcgaggtgg tgccccacat cgcccgcacg
     3061 gcgcgcggca agcggcgcct gatgatccag cacgtcacat ccgaagtgga ggagggcagc
     3121 cgtgtctaag aaggctccag cgctgatcat gctcggtccc gatcccgagg cccgcggcgg
     3181 catcgcttcc gtccagcgca tctggctgca ggccgggctg ctcgacgaac gtcccgtccg
     3241 cgtgctcacc acctaccggg atggcagcgc cctgcgcaag ctcggcgcct tcgtcactgc
     3301 gctgctgcgc ttcctgctgc tcctggcggc cgggcgcgtc gccgcggtgc acgtccattc
     3361 cgcctccaac gccagcttcc ggcgcaagag cgtcttcctc gccctggccc gcctggccgg
     3421 caagccctac gtcttccacc tccacggcgg cgccttcgac cattactacg cgggcgcctc
     3481 cttcctcgag cgggcctggg tggatcgcac cctgcacggg gcggccgtgg tgttcaccct
     3541 gtccgaaggc tgggccaact ggctgcgcat caacgtcggc caccccgacg tgcgcgtgct
     3601 gcccaatccg gtgctgcccg tcaccttgcc ggccggcgtc gcgcgggagc ccgccaccct
     3661 gttgttcctc gggcgcctgg aagacgccaa gggcgtcttc gtgctcgtca aggccattgc
     3721 ccgtctggca cgcaaattcc ccgacctgcg cgcggtcatc gccggtgacg gtgacatccg
     3781 cgccgtgcgc gacgccgccg agcgggccgg tgtgcttgac cgcatcgaga cgccgggctg
     3841 ggtggatggc ggcaagaagc tccgcctcct cgcgcgggcg ggcgtcttcg tgctgccgtc
     3901 ccgcttcgag ggcctgccca tgtccgtgct ggaagccctg gccgccggtc tgccggtggt
     3961 ggccagccgc gtcggcggca ttccccaggt ggtgcgcaac ggcgacaacg gcgccctgca
     4021 cgaagccgac gacgtcgcat ccctggcgtc cgccctcgaa cgcgttctgg ccgatccggc
     4081 tgcggcacgg gccatgggcg agcggggccg ccgccgggtg ctcgccgact acggcgtgga
     4141 gcgcatccgc gccctcctca atgcggtctg ggattcgctg atgacccccg cttcggagcg
     4201 tttcgcgcga tgatcgtctc gtgccgctcc ccgctgtatc aactggcgcc ggtcgccctg
     4261 caggagcgtc tgctctcctg gcagacgcgc ctgcggcgct ggctgacccg gcctgcggcc
     4321 ctcgcccaga cccgtcacgc catcgccgac agccagtggc tgtcgcgcga gggctggcaa
     4381 tccctgcagt tcgccgccgt gcgggatctg gccctccatg ccgcccgcca tacccgttat
     4441 taccaggacg tcttcgcctg cctgggcacc gacccgtcgg cctggacctc cctggacgat
     4501 ctgagccgcg tgccggctct ctccaaggac gaagtgatct ccatcggtga tgggctgctc
     4561 gcccgcgacg gcacctggct gcgcttccag ggctccacca gcggcaccac cgggcgcgcc
     4621 atgcgcggct ggcgcgatca ggcctcggtg accttcgagc aggccttcgt ggatcgccag
     4681 gcggagtggg ccggttaccg gcccggcgac cggcgtgcct ggctgcgcgg cgatcccatc
     4741 gtgccgacgc ggcagcaggg cggccccctg tggcgcttca accgggcgga caacatgctg
     4801 atgctgtcct cctatcacct gtcccgctac aacgggctgg cctatctgca cgccctcgag
     4861 agcttcgacc cggtcctgct gcaggcctat ccctcgtccg tggcgtatct ggcgcgcctg
     4921 ctggaagacg aaggccgcca ttaccgcggg ccggccctgc gcggcatcgt cacctcgtcc
     4981 gagaccctcg gcgccgacga ccgccgcgtc atcgccgagc gcttcggctg ccccgtcttc
     5041 gactggtacg gcgcctttga acgggtggcc gccatcggca cctgcgaaca cggccgctac
     5101 cacgtgatgg aggactacgg cgtcaccgaa ttcgaggaca acggcgacgg caccgccaat
     5161 ctcatcggca ccggcttcgg caatcgcagc atgcccctgc tgcgctaccg ctcggatgac
     5221 cgcgtggtgc tggccgaccc cgacttccag tgcggatgcg ggcgcagctt ccgcgtcgtg
     5281 gagcgcatcc tcggccgcgt ggacgacgtc atccgcacgc cggatgggcg ccacgtggtg
     5341 atgctggact ggatcttcgc ggggctcttc ggcctcatcg aggcccaggt cgtgcaggaa
     5401 cgcctcgacc tcgtgcgcat ccgcatcgtc gtcgcgccgg aattctcggc ccaggacgaa
     5461 caacacctcc tgcaacgcgc ccgcgagcgc ctcggtccgc aggtgacgct gcgcatcgaa
     5521 cgggtgcccg ccatcgaacg cacgcgcaac ggcaagttcc ggcagatcgt cagccggatc
     5581 cactccaggc aggatatccg atgagcaaac tcacccgcgt gggctggtac tggaaccggc
     5641 tgcgctgcat gagcgtcgcc gaaatggtcg agcgcgcctc gacctccgcc ggccacgccc
     5701 tcgaacggcg acgccggccc gtgccgatcc cgccgcccga cctgacgcgg cgcggcgcct
     5761 gctggttgcc cgcccacgag ctgccccccg tgccgccggc cgtcatcgac cgggccgacg
     5821 agatcctcgg cgggcgctgg cgcatcttcg cgcggggccc cgtggccctc ggcctgccgc
     5881 cggaatggaa ccgcaatccc cacaacggcg ccgtcctgcc catggttttc ggcaagacca
     5941 tggccttccg cgacaccacg gtcatcggcg acatcaagta cctgtgggag cccaaccgcc
     6001 acctggagct ggtcgtcctc gcccaggcct ggaaagccac cggcctgcgc aaatacctgg
     6061 acgccctcgg cacgctcctc accagctggt tccgccagtg cccgtatccc ctcggtccca
     6121 actggtccag cgccctcgaa gccggcatcc gcctcatcaa ctggtccatc gtgtggcagc
     6181 tcatcggcgg cttcgagtcg cccctgttcg ccccgccgga aggcgtcgtc ctgctgcgcg
     6241 actggctcga cagcatctgg cgccacgccg agttcatccg cggccaccgc tccctgcact
     6301 catcggccaa caaccacctg atcggcgaac tgtcgggcct ctacgtggcc agcgtcacct
     6361 ggccctgctg ggaggacgca ccccgctggc gcgaagccgc ccgcgccggc ctggagaccg
     6421 aggtcctgcg ccagaacgcg ccggacggcg tcaatctcga gcaggccacg tcctaccagc
     6481 agttcgtgtg ggacttcctc ctcttcgccg gcctcgccgc gcgggccacc ggggatgact
     6541 tctccaccgc ctactggcaa cgcatggagg ccatgctcga ctacgtggcc gccatcatgg
     6601 acacgggcgg gcaggtgccc cagttcggcg acgccgacga cggcctcgcc tgcgggctgt
     6661 ccctctgcgc cgaccgcgac cccttccgct cccagctcgc caccggcgcc gtgctcttca
     6721 aacgctccgc ctttgctcgc aaggtcggcc gcttcgacct gcgcagcgtc tggctcctgg
     6781 ggcgcgccgc cgccgacgac ttcgccaccc tgctggtgga ccggagcgaa cccctcacgc
     6841 cgcgccaggc cttcccggac ggtggcgtgt atgtcctcgg cagcgccctc gacaccccgc
     6901 gcgaagtgcg cgtcgtcgcc gacgccggcc ccctcggcct cggcggcatc gccgcccacg
     6961 gccacgccga cgccctgtcc ttcaccctgt cgatggacgg gcgcgagttc ctcgtggatc
     7021 ccggcaccgg cacctaccac ggtccccgcg cctggcgcga cggcttccgc agcaccgccc
     7081 tgcacaacac cctcaccgtg gatggcgagg accaggccat caccggcggc aagttcatgt
     7141 ggatccgcaa atacgaggtc agcgtgctcg aatggtccag cagcgccgac gccgaccgcc
     7201 tggtggcgga gcacgacggc taccggcgcc tcgccggcca ccccgtgcat cgccgtgcct
     7261 ggcgcctcga caagcgcagc ggcaccctct cggtgcgcga cgagctgggc gcccccgcgc
     7321 cccgccccgt ggccctgaac tggcactttg ccgaaaactg cgtgatcacc tgcacgcccg
     7381 acggcctcgt cgtcggacac gacggcgtca ccctgctggt gacgctcccg cccggtggcg
     7441 agaccaccgt catctgcggc tccgacgtcc ccctgtgcgg ctgggtgtcc cgcggctacg
     7501 acctgcgccg ggccagcacc accgtcatct ggcgcggcca cctcggcccc ggtcaggccg
     7561 ccgagaccgt cttccgccgc ggctgagccg ccaaccgtcc gcgtcgcccg tcgtgccggc
     7621 gggcggacgg cctcagcctg gcggcgcgtc catccgcgag ccggcagcga aacgatgcta
     7681 gtcttccggc ggcccggccc caccgggcac gcccgctgcc ttccggagga cgccccatgg
     7741 gactcacccc gatccgcctg ctgctcttcg ccatgatcgt gctcgccctc cagtacggct
     7801 ggaagcacat gccccatccc ggcgccggcg gcagcccgac cgccgaagcc ctccgcagca
     7861 tcgccagcac cgtgcagccc ggcgaggtca cgatgtactc caccaccgaa tgcgcctact
     7921 gcgcccaggc ccgcgcctgg ctcacccagt acggcttcgc cttcaccgag tgcaacatga
     7981 gcctggatcg gcgctgcgaa cgggaattcc tcgcctacgg cgccaccggc accccctacc
     8041 tggtcatccg ccgcggtggt cgcacctatc acatgaagga cggcttcgat tcggaggagt
     8101 tcctgagggt gctgaagggc taggggcgcc ggttccacca gcttgtagcc gacgccgcgg
     8161 acgctgcgta tccact
//
LOCUS       DAOQPA010000137         8137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4886_length_8137_cov_17.120762, whole genome shotgun sequence.
ACCESSION   DAOQPA010000137 DAOQPA010000000
VERSION     DAOQPA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_8137_cov_17.120762"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PKX01_14825"
     CDS             <1..1323
                     /locus_tag="PKX01_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HMZ77455.1"
                     /translation="ASRWGWHTFNAHQVAFTSGIWVMMCQTLVPTQRINDGAYFATEF
                     RLPKGTWCNPDDRRTGHAYAWHFLVSGWAALWRGLSQSYFSRGYLEEVNAGNANTSNW
                     LQGGGINQDGEIHAVNSFEASSCGTGACAIKDGLNHAAAIWNPEGDMGDIEIWEMAEP
                     LLYLGRNVKANSGGYGKYRGGCGFETLRMVWNAQDWTMFFMGNGFMNSDWGMMGGYPS
                     ATGYRFEAHKTGLEKRIEIGDSLPLGGDLDPSDPDYERHLDATATVKRDKQCITTEDC
                     YANHDLYLNYLRGGPGFGDPIDREPKAIEADLNQKFLLPEYARKVYGAVFTEDGGVFT
                     VDVAKTAERRTEIRKERLARALPTREWMKAERERILNKHASTQVKHMFATSFALSEKF
                     TQQFKDFWHLPADWQLLEDELDVPTYGSKHRMDLSLLPDVTTVVQVEE"
     gene            1347..1856
                     /locus_tag="PKX01_14830"
     CDS             1347..1856
                     /locus_tag="PKX01_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HMZ77456.1"
                     /translation="MSTYTNEQVTHLVEGTLDWETTFRMLSMPKDHGRFEQYLAALQA
                     KVDFADRIILPLGPHLYIVQQASTRKWVTQCDCGHVFGDYRENWKLNASVYVRRTEEA
                     MTEVYPRLMAPDTEWQVYREYYCPQCGAMHDVEAPTPWYPVIHDFEPDIEAFYKEWVN
                     LPLPERAPD"
     gene            1943..3340
                     /locus_tag="PKX01_14835"
     CDS             1943..3340
                     /locus_tag="PKX01_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HMZ77457.1"
                     /translation="MCKVYPNAAAALDGLLFDGMTVAAGGFGLCGIPENLIAALLAAG
                     TRELTIVGNNAGVDDFGMGLLLKSRQVKKVVASYVGENKEFERQVLAGELELELVPQG
                     TLAERLRAGGAGIPGFYTRTAFGTLLADGKETRRFGSKDYVLEPAIRADVSIVKAWKG
                     DRAGNLVFRKTARNFNPMIATCGSLCIAEVETLVDVGELDPDEIHTPGIYVNRIIAGA
                     DYQKRIEFRTVAGSASPARHDPVRDRMARRAAQELRSGYYVNLGIGIPTLVANHIPPG
                     VQVTLQSENGLLGIGPSPEATAVDADLINAGKQTITTLPGSSFFSSADSFAMIRGGHI
                     DLSILGGLEVSEQGDLANWMVPGRMVKGPGGAMDLVSGVGRVVVLMEHCAKDGSPKIL
                     RECSLPLTGKGVVDTLITDLAVFKVKEGGGLLLTELQPGVSVEDVRERTGCGFSVAPQ
                     VASGVPATIRTGART"
     gene            3398..3808
                     /gene="pcaC"
                     /locus_tag="PKX01_14840"
     CDS             3398..3808
                     /gene="pcaC"
                     /locus_tag="PKX01_14840"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HMZ77458.1"
                     /translation="MSERFERGLAMRRQVMGDAHVERAFASATGFSRPLQEFMTEHAW
                     GTVWCRDGIDLKTRSLITVAMLAALGRGQELAGHVRGALHNGASADEVREVLLHAAVY
                     VGIPLAAEAVRIAEAVVRERPDASPPLDSPSPSR"
     gene            complement(4014..4841)
                     /locus_tag="PKX01_14845"
     CDS             complement(4014..4841)
                     /locus_tag="PKX01_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ77459.1"
                     /translation="MNKHTFAALTLAGFGLAFALPGHAGSIAVDGNFSDWGISNNGTA
                     AGWKPTSGIFYTVEDQDNSNSGYLSPGWGGQAYDAEALYATWSTHANGQTYLDIALIT
                     GHDPSTVTNGSTSFGRGDFAIDFGRDGSWDYGILTADRSTTLHQGDVVATTNADWAKG
                     LWTAPGVYDPAHSQYITAVKTGTDVGDAALAISTVFTNMGVLGGNHWFYELEIPVTAF
                     GQHWTSSGPTEAFTLEWTMLCSNDIITLDPSRVPEPATAALLLGALGVLGAARRQRR"
     gene            complement(5013..5450)
                     /locus_tag="PKX01_14850"
     CDS             complement(5013..5450)
                     /locus_tag="PKX01_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HMZ77460.1"
                     /translation="MAITWILVANASLARLYANLGPNKGLQLIKELVHPQSRMKNADL
                     VTDRAGLLQAGSGHGAREPATPPKQNAARSFAQMLAKELYQGRSRNKFGRAILVAPPA
                     FMGMLNHEMDRNTAQMVSDRLEKDYTKTPEPALCGRLENCLFV"
     gene            complement(5553..6338)
                     /locus_tag="PKX01_14855"
     CDS             complement(5553..6338)
                     /locus_tag="PKX01_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HMZ77461.1"
                     /translation="MFAAFRRWRRQRAADRLTVTAAQWDAVEATLPFLAWLPADDRPA
                     LRRMALEFLADKEIHGAHGLQVTDAMLLSIALQACLPVLHIGLDAYAGWVGIVVYPGD
                     FVIPRQDVDEDGVVHEYDDEVLGEAWDGGPVLLSWFDGDGDDAPDGVNVVIHEFAHKL
                     DMGNGEVDGFPALPPGMSPQAWADAFRPAYEDFCRRVDAGEDTALDPYASQDPGEFFA
                     VLSEAFFETPGRLAAEYPAVYEQFSRYYRQDPRAQEAAASTKI"
     gene            6444..>8137
                     /locus_tag="PKX01_14860"
     CDS             6444..>8137
                     /locus_tag="PKX01_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HMZ77462.1"
                     /translation="MVAVTHSISQSTSASSPQELLCEGLQPGDAVRVAEALAWAHSLY
                     GARTLGTGEPVWEHALGTALIAASLRLDADTRIAALCFAVGDVLDHPGDRIAEHFGAG
                     VAGLVDGLRRLNGLRVLTRLTALASAPEIRAQTEVLRKMLLAMVEDIRVVLLRVASRT
                     QTLRYFTDHPGPTRDAMARESLDIYAPLANRLGIWQLKWELEDLSFRFLEPDTYKRIA
                     KMLDERRVEREQFIVDAVERVKREVAAIGVEAEVYGRPKHIYSIYNKMRSKRLDFSQI
                     YDVRALRVLVHEVKDCYAVLGIVHQMWTPIPKEFDDYISMPKGNNYQSLHTAVYAGDG
                     RSLEVQIRTHDMHHHAELGVAAHWRYKEGSGAAAGAYDEKIALLRSLLSWRDEVTDAA
                     DWLEQFKRASLDDTIYVLTPQGRVIDLQRGATPIDFAYRVHTDLGHRCRGAKVNGQLV
                     TLNTPLESGQTVEIMSAKEGGPSRDWLNPHQAYVATPRARQKIKQFFSAQEEEELLVR
                     GRSVLTREMQREGFAQTNIDELATRLGFKTAEAMFIAAGRGELGPRAIQTALRGADA"
BASE COUNT         1409 a         2709 c         2723 g         1296 t
ORIGIN      
        1 gcgagccgct ggggctggca caccttcaac gcccaccagg tggcattcac ctcggggatc
       61 tgggtgatga tgtgccagac cctggtgccg acccagcgca tcaacgacgg cgcctacttc
      121 gccaccgagt tccgcctgcc caagggcacc tggtgcaatc ccgatgaccg ccgcacgggc
      181 cacgcctacg cctggcactt cctcgtctcc ggctgggcgg cattgtggcg gggcctgtcc
      241 cagtcgtact tcagccgggg ctacctggag gaggtcaacg ccggcaacgc caatacctcc
      301 aactggctgc aggggggcgg catcaaccag gacggcgaga tccacgcagt caacagcttc
      361 gaggcaagct cctgcggtac cggcgcctgc gccatcaagg acgggctcaa ccatgcggcg
      421 gcgatctgga acccggaagg cgacatgggc gacatcgaga tctgggaaat ggccgaaccg
      481 cttctctacc tgggacgcaa cgtcaaggcc aactccggcg gctacggcaa gtaccggggc
      541 ggctgcggct tcgagaccct gcgcatggtc tggaatgccc aggactggac catgttcttc
      601 atgggcaacg gcttcatgaa cagcgactgg ggcatgatgg gcggctatcc gtcggccacc
      661 ggctaccgct tcgaggccca caagaccggg ctcgagaagc gcatcgagat cggcgacagc
      721 ctgcccctgg ggggcgatct cgatccgtcc gatccggact acgagcgcca cctcgacgcc
      781 accgccacgg tcaagcgcga caagcagtgc atcaccaccg aagactgcta cgccaaccac
      841 gacctgtacc tcaactacct gcgcggcggc cccggcttcg gtgatcccat cgaccgtgag
      901 ccgaaggcca tcgaggcaga cctcaaccag aagttcctgc tcccggaata cgcccggaag
      961 gtctatggcg cggtgttcac cgaagacggg ggcgtgttca ccgtcgatgt cgccaagacc
     1021 gccgaacgcc gcaccgagat ccgcaaggaa cgcctggccc gcgccctgcc gacccgcgaa
     1081 tggatgaagg cggaacgcga gcgcatcctc aacaagcacg cctcgaccca ggtcaagcac
     1141 atgtttgcca ccagcttcgc cctgtccgag aagttcacgc agcagttcaa ggacttctgg
     1201 catctgccgg ccgactggca gctgctcgag gacgagctgg atgtcccgac ctatggttcc
     1261 aagcaccgca tggacctgtc cctgctgccg gacgtcacca ccgtcgtcca ggtcgaagaa
     1321 taaccaccga atcaaggagt caatcaatgt ctacctacac caacgagcag gtcacccacc
     1381 ttgtcgaggg cacgctggac tgggaaacca ccttccgcat gctgtccatg cccaaggacc
     1441 acggtcgctt cgagcaatac ctggcagccc tgcaggccaa ggtcgatttc gcggaccgca
     1501 tcatcctgcc gctcggcccg cacctctaca tcgtgcagca ggccagcacc aggaaatggg
     1561 tgacccagtg cgattgcggc catgtctttg gcgactaccg cgagaactgg aagctcaacg
     1621 cctcggtgta cgtgcgccgc accgaagagg ccatgaccga ggtctatccc aggctgatgg
     1681 cccccgacac cgagtggcag gtgtatcgcg agtactactg cccccaatgc ggcgccatgc
     1741 acgacgtcga ggcgccgacc ccgtggtatc cggtgatcca tgacttcgag ccggacatcg
     1801 aagccttcta caaggaatgg gtgaatctgc cgctgccgga gcgggcgccg gactgagcct
     1861 gcgattgaac ggcccccggg gccgtcaagc ggggacgggc gggggcctgt ccccgcccga
     1921 caagaacagc ggagacacac ccatgtgcaa ggtttatccc aacgctgccg cagcgctgga
     1981 cggactgctc ttcgacggca tgaccgttgc ggccggcggc ttcggcctct gcggcatccc
     2041 cgagaacctc attgccgcgc tgctggcggc gggcacccgc gagctgacca tcgtcggcaa
     2101 caacgcaggc gtggacgatt tcggcatggg actgctcctc aagtcccgcc aggtgaagaa
     2161 ggtcgtcgcc tcctacgtgg gcgagaacaa ggagttcgag cgtcaggtgc tggcgggcga
     2221 actggagctg gaactggtgc cccagggcac cctggccgaa cgcctgcggg ccggtggtgc
     2281 aggcattccc ggcttctaca cgcgtaccgc ctttggcacc ctgctcgccg acggcaagga
     2341 gacccgccgt tttggcagca aggactacgt gctggagccg gccatccgcg ccgatgtctc
     2401 catcgtcaag gcgtggaagg gggaccgggc gggcaacctg gtcttccgca agaccgcccg
     2461 caacttcaac ccgatgatcg ccacctgcgg cagtctgtgc atcgccgagg tcgagacgct
     2521 cgtggatgtc ggcgaactgg acccggacga gatccacacg ccgggcatct acgtgaaccg
     2581 gatcatcgcg ggggccgatt accagaagcg gatcgaattc cggaccgtgg ccggcagcgc
     2641 ctcgcccgcc cgccacgacc ccgtgcgcga ccgcatggcc cgccgcgccg cacaggagct
     2701 gcgcagcggc tactacgtga acctggggat cggcattccg accctggtcg ccaaccatat
     2761 cccgccaggt gtccaggtca ccctgcaatc ggagaacggc ctgctgggca tcggcccgtc
     2821 gcccgaggcc acggcggtcg atgcagacct catcaacgcc ggcaagcaga ccatcacgac
     2881 cctgcccggc agcagcttct tttccagcgc ggattccttc gccatgatcc gcggcggtca
     2941 catcgatctg tccatcctgg gcggcctgga ggtgtcggag cagggcgatc tggccaactg
     3001 gatggtgccc ggccggatgg tcaagggccc cggcggcgcg atggacctgg tctccggtgt
     3061 cgggcgggtc gtcgtcctga tggaacactg cgccaaggac ggttcgccca agattctccg
     3121 cgaatgctcg ctgcccctga ccggcaaggg cgtggtggac accctcatca ccgatctggc
     3181 cgtgttcaag gtgaaagagg gcggcggcct gctgctgacc gaactgcaac ccggcgtcag
     3241 cgtggaggac gtccgcgaac gaaccggctg cggcttttcc gtggcaccgc aggtcgcaag
     3301 cggcgtgccg gcgacgatcc gcactggagc acggacatga tgggcgccgc cagcagcgtg
     3361 cgtcccgtcc gggacggcac cgtgagggag acacagcatg tctgaacgtt tcgagcgcgg
     3421 tctggcgatg cgtcgccagg tgatgggcga cgcccatgtc gagcgggcgt ttgcatcggc
     3481 caccggcttc tcccgtcccc tgcaggagtt catgaccgag cacgcgtggg gcacggtgtg
     3541 gtgtcgcgac ggcatcgacc tcaagacccg cagcctgatc accgtcgcga tgctcgccgc
     3601 cctcgggcgc gggcaggagc tggcagggca tgtgcggggc gccctccaca acggcgcgag
     3661 cgccgacgag gtccgcgaag tcctgctgca tgccgccgtc tatgtgggta ttcccctggc
     3721 ggccgaggcg gtccgcatcg ccgaagccgt cgtccgggag cggcccgacg catcgcctcc
     3781 cctcgattct ccgtcgccat cgcgttgaac gctcgaagac gcgaggcaga ccgacggtga
     3841 atctttggcc cgctccggcg ggccgctttt ttgaagcgac gcatgggctg cacgggccgg
     3901 cctacggcgg cccccacacg ctgcacgtac caaaaaaaag ccggcccgga ggccggcaaa
     3961 ttcctaggtg ccagagaatg aaatcggtgg ggcagcgcct gcgcccgtgg ggatcagcgg
     4021 cgctgccggc gtgccgcgcc cagcacgccg agggcgccca gcagcagggc ggcggtggca
     4081 ggttccggga cgcggctggg gtcgagggtg atgatgtcgt tcgagcacag catggtccac
     4141 tcgagggtga aggcctcggt gggcccgctg gatgtccagt gctggccaaa ggcggtgacc
     4201 ggaatctcca gctcgtagaa ccagtggttg ccgcccagga cgcccatgtt ggtgaagacg
     4261 gtggagatgg ccagcgcggc atcgcccacg tcggtgcccg tcttcaccgc ggtgatgtac
     4321 tgggaatggg ccgggtcata gacgccgggt gcggtccaca agcccttggc ccagtcagcg
     4381 ttggtggtgg cgaccacgtc gccctgatgc agggtggtgg agcggtcggc ggtgaggatg
     4441 ccgtagtccc agctgccgtc gcggccgaag tcgatggcga agtcaccgcg gccgaaggac
     4501 gtgctgccgt tggtgacggt gctcgggtcg tggccggtga tcagggcgat gtccaggtag
     4561 gtctggccgt tggcgtgggt gctccaggtg gcgtagagcg cttccgcgtc gtaggcctga
     4621 ccgccccagc cggggctcag gtagccgctg ttggaattgt cctggtcttc caccgtgtag
     4681 aagatgccgg aggtgggctt ccagccggcg gcggtgccgt tgttggagat gccccagtcg
     4741 ctgaagttgc catccacggc aatggagccg gcgtggccgg gcagggcgaa ggcgaggccg
     4801 aagccggcga gggtcagggc cgcgaaggtg tgcttgttca tgattccgca ccacttgaaa
     4861 tgtcgatggc acggagctta ggcgcctgaa atccgcatca ggcggtactt ctccacggaa
     4921 tgcgggtggg atgggggaaa tgaaaaggcg gtcgcgaccc gtgtcacgcc cgccttgaag
     4981 atgcgcggtg cccgcacggg gcacccgtgt gttcagacga agaggcagtt ttccaggcga
     5041 ccgcagaggg cgggctccgg cgtcttggtg tagtcctttt cgagccggtc ggagaccatc
     5101 tgcgcggtgt tgcggtccat ctcgtgattg agcatgccca tgaaggcggg cggggcgacg
     5161 aggatggcgc ggccgaactt gttgcgactt cggccctggt agagttcctt cgccagcatc
     5221 tgggcgaagg agcgggcggc attctgtttg ggcggggtgg cgggttcgcg ggcgccgtgt
     5281 ccgctgccgg cctggagcag gcccgcgcgg tcggtgacga gatcggcgtt cttcatgcgg
     5341 ctttgcggat ggaccagttc cttgatgagc tgcagtccct tgttcggtcc caggttggcg
     5401 tacagacggg ccaggctggc gttggcgacc agaatccagg taatagccat gaatcgctct
     5461 ccatagaatt gcaaaatgcc gtggacggct ttctcactgt agatgcgatc cggccggcat
     5521 tcaaagtgac gttgcggaaa tgtgtcggtc cgttaaatct ttgttgacgc cgccgcctcc
     5581 tgcgcccgtg gatcctgccg gtagtagcgg gaaaactgtt cgtaaacagc agggtattcc
     5641 gcagccaggc ggcctggcgt ttcgaagaag gcctcgctca gcacggcgaa gaactcgccc
     5701 gggtcctggc tggcgtaggg atcgagggcg gtgtcctcgc cagcgtctac gcgtcggcag
     5761 aagtcctcgt aggcgggccg gaaggcgtcg gcccaggcct ggggcgacat cccgggcggc
     5821 agcgcgggaa agccgtccac ctcgccgttg cccatgtcca gcttgtgggc aaattcgtgg
     5881 atcaccacgt tgacgccgtc cggcgcgtcg tcgccgtccc cgtcgaacca ggacagcagc
     5941 accggcccgc cgtcccaggc ctcgccgagc acctcgtcgt cgtattcatg caccacgcca
     6001 tcctcgtcca catcctgccg cggaatcacg aaatcccccg gatagaccac gatgcccacc
     6061 cagcccgcgt aggcgtccag gccgatgtgg agcaccggca ggcaggcctg cagggcgatg
     6121 gagaggagca tcgcgtcggt gacctgcagg ccgtgggcgc cgtggatctc cttgtcggcg
     6181 aggaattcca gggccatgcg gcgcagggcc gggcggtcgt cggcgggcag ccaggcgagg
     6241 aagggcaggg tggcctccac agcgtcccat tgggcggcgg tgacggtcag gcggtcggcg
     6301 gcgcgttgac ggcgccagcg gcggaaggcg gcaaacatgg tgcgatggta gccgaggcgg
     6361 gaaccgggcg gcgggtgtgg agtccatcac tgcggacgat ccaggtcaaa cccctgccat
     6421 tctggctgta ggataatgtc gtcatggttg ccgttaccca ttccatctcc cagtcgacct
     6481 ccgcgtcatc cccccaggaa ctgctctgtg aaggccttca gcccggcgac gccgtgcgcg
     6541 tggcggaagc cctcgcgtgg gcccattcgc tgtatggggc gcgcaccctg ggcaccggcg
     6601 aacccgtgtg ggagcacgcc ctcggcacgg ccctgatcgc ggcctccctg cgcctcgacg
     6661 cggacacccg catcgccgcc ctgtgctttg cggtgggcga tgtgctcgat catccgggcg
     6721 accgcatcgc cgagcacttc ggcgccggcg tggcggggct ggtggatggc ctgcgccgcc
     6781 tcaacggcct gcgcgtgctc acccgcctga cagccctcgc cagcgcgccg gagatccgcg
     6841 cccagaccga agtcctgcgc aagatgctgc tggccatggt ggaagacatc cgcgtggtgc
     6901 tgctgcgcgt ggcctcccgc acccagaccc tgcgctactt caccgatcac cccggcccca
     6961 cccgcgacgc catggcgcgg gaaagcctgg acatctacgc cccgctggcc aaccgcctgg
     7021 gcatctggca gctcaaatgg gaactggaag acctgtcctt ccgttttctg gagcccgaca
     7081 cctacaagcg catcgccaag atgctcgacg agcgccgcgt ggagcgggag cagttcatcg
     7141 tggatgcggt tgagcgggtc aagcgcgaag ttgccgccat cggcgtcgag gccgaggtct
     7201 atggccggcc caagcacatc tacagcatct acaacaagat gcgctccaag cgtctggatt
     7261 tctcccagat ctacgacgtg cgcgccctgc gcgtgctggt gcacgaggtc aaggactgct
     7321 acgccgtgct gggcatcgtg catcagatgt ggacgccgat ccccaaggag ttcgacgact
     7381 acatctccat gcccaagggc aacaactacc agtccctgca caccgccgtg tatgccggcg
     7441 atgggcgctc gctggaggtg cagatccgca cccacgacat gcaccatcac gccgagctgg
     7501 gcgtggccgc ccactggcgc tacaaggaag gctccggtgc cgccgccggc gcctacgacg
     7561 aaaagatcgc cctgctgcgc agtctgctct cgtggcgcga cgaggtgacc gacgccgctg
     7621 actggctgga acagttcaag cgcgcctccc tggatgacac catctacgtg ctcacgccgc
     7681 aggggcgggt catcgacctg cagcggggcg ccacgcccat cgacttcgct taccgcgtgc
     7741 acacggatct gggccaccgc tgccgcggcg ccaaggtcaa tggccagctc gtcaccctca
     7801 acacgcctct ggaaagcggc cagaccgtcg agatcatgtc cgccaaggaa ggcggcccct
     7861 cccgcgactg gctcaacccg caccaggcct acgtggccac gccgcgggcc cggcagaaga
     7921 tcaagcagtt cttcagcgcc caggaagaag aggagctcct cgtccgcggg cgcagcgtgc
     7981 tcacccgcga aatgcagcgg gaaggcttcg cccagaccaa catcgacgaa ctggccacgc
     8041 gcctcggctt caagaccgcc gaagccatgt tcatcgccgc cggccgcggc gagctggggc
     8101 cccgcgccat ccagaccgcc ctgcgcggcg cagatgc
//
LOCUS       DAOQPA010000138         8030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_4988_length_8030_cov_18.629091, whole genome shotgun sequence.
ACCESSION   DAOQPA010000138 DAOQPA010000000
VERSION     DAOQPA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_8030_cov_18.629091"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1351)
                     /locus_tag="PKX01_14865"
     CDS             complement(152..1351)
                     /locus_tag="PKX01_14865"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HMZ77463.1"
                     /translation="MHYGEFDTASLMFITPRPELIFVRGDGSWLHDDTGKRYLDFVQG
                     WAVNCLGHCPAEIREALADQAARLINPSPAFFNGPMTELAGLLAEHSCFDRVFFANTG
                     AEANEGAIKLARKWGRMHKDGAYEIITFEHSFHGRTLATMSASGKPGWDTLFAPQVPG
                     FPKARLNDLDSVGALIGDKTVAIMLEPVQGEGGVIPAEPGFLQALRDLTRAHGLLLIV
                     DEVQTGMGRTGRLFAHEHAGIEPDIMTLGKGIGGGVPLSALLAKEAVCCFQPGDQGGT
                     YNGNPLMTAVGAAVLRRLLSDGFLARVDTLGRYLESRLQALVTRHALAGERGAGLLRA
                     LILDSDRGPALVKAALDYAPEGLLLNAPRPNLLRFMPALTVTEAEIDRMIEILDTLLG
                     AVRADAG"
     gene            complement(1422..3662)
                     /locus_tag="PKX01_14870"
     CDS             complement(1422..3662)
                     /locus_tag="PKX01_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77464.1"
                     /translation="MMHTPDPSQPPPPLFDRENPGQLLRTMGGISLLLTLIVGVSVFA
                     QHDVSPIHGLTALGVCIALCAGLLGFRRHPDRLPSRCVLWGAWLIILIGAAFTGGNRT
                     TSLSAIPVLIVLVGWLFGMRHALLMTAVSILALLGMSLSEQYDWLPMGGSSPPLSRWM
                     VLSNVLLLTAFLTLHSRRLYLSRYQALHRLSATLGLLTEGTPIMLAAVDGRGCYSYVN
                     QNYAHFHGLAPTALRGSLVDAVLGDGIQQHNLARLDAGGGSATFRSRRHNRQTGDERW
                     IETCLRSATATDGRTDGYYALLRDITDEVRTEEEIRFLAYHDALTGLPNRILLADRLR
                     QAIARAARDDTLVAVCYLDLDRFKFFNDTWGHTVGDEILVQVAGRLRDSLRGSDTVAR
                     LGGDEFLVLIGGIADRQEMPAALARLLGTVSQPVVLEDGAEIRISASAGVALFPRDGE
                     EPDVLLRHADQAMLLAKQSGRARYALFDTELEQRNHHLQRIVSSVDRGLDGGQFRLFF
                     QPKVNLRLSSVIGAEALIRWEHPDEGLLLPDAFLPWLESSPVGVRLDRWVLAAALDQM
                     SRWHAAGLDIPVSINVSGHHLLAPDFVDHLTAQLIAHPSVAPERLELEILETTAMEDV
                     DQVARVIRACTALGVSFALDDFGTGYSSLTYFRRLPARTLKIDRSFVRDILADPEDLA
                     IVKGVVALARSFGRQVIAEGVETPEHCGPLLSLGCDCVQGYGIGHPMPAAEFPGWVRT
                     WKSVNG"
     gene            complement(3715..5442)
                     /locus_tag="PKX01_14875"
     CDS             complement(3715..5442)
                     /locus_tag="PKX01_14875"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HMZ77465.1"
                     /translation="MTAATTAPKNARLQARIGHASLTGPRPRNEDFCGAVTPDGAELD
                     TKGILAVVADGVGGHANGREASEYTVRGLLSDYYATPDTWAVNKALDTVLNALNRWLV
                     SQASRTREAAGMATTLSAIVVRGRRFYTAHVGDSRIYRLRDGRFEQLTTDHLWEHPEL
                     KNVLSRAIGLDPHLSVDYADGDLEPGDVFALMSDGVWNQLGDKRIADILRSQDEPQAA
                     AARLAGEAEDGGSQDNCTALVVRVDALPPDRLRDTIARLGQLTLPPRLKPGQELDGLT
                     VDALLHESIATLLYRVHDAQGHPRVLKTLRPERNDPEASAALAHEEWLARRVNDAYFP
                     QVILHPARAHLYYLMSWHEGATLKARLESGHRFGLGEIVSLGERLLKGLAGLHRLSII
                     HRDIKPDNLHLDNDGRLRILDLGVAASDSRGDGQQFHEINNPGTPSYMAPELYGPTPT
                     AANEQTDLYAVGVTLYELLTRKYPYGEIEPFQNPKFGDPVPPTRFRPDIPAWMEAVLL
                     KAVARDPRARFETAEEFLLALERGAHRPLVVPRKSPLLQRDPQLGLKLLAAASLTLNI
                     LLIYLLLVR"
     gene            complement(5637..6851)
                     /locus_tag="PKX01_14880"
     CDS             complement(5637..6851)
                     /locus_tag="PKX01_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HMZ77466.1"
                     /translation="MDRKSFLQAGHTPTLIAAFLYFDLAFMVWVLLGPLGVQIAKDLG
                     LSHAEKGLMVATPVLAGAALRIVMGVLVDHLKPKTAGAIGQVIVIGALAAAWKLGIHN
                     YEETLILGFFLGVAGAAFAVALPLASRWYPPEHQGTALGIAGAGNSGTALAALFGPGL
                     AAAYGWNNVFGLALIPLSIVFVLYLFMAKDAPECPPAKSLTEYLAVLKDKDAWWFMFF
                     YSVTFGGFVGLASSLTIYFNTQYGLDPKTAGFYTAACVFAGSLVRPIGGNVADRIGGI
                     KSLTVMYVLAAIFLAIVSFGMPESWQALLVFVCAMLSLGMGNGAVFQLVPQRFRKEIG
                     VMTGLVGMAGGVGGFYLASSLGYSKQVTGSYQSGFLIFAALAIAALVGLTAVKSRWRT
                     TWGAPHLTSAKI"
     gene            complement(6997..7308)
                     /gene="nirD"
                     /locus_tag="PKX01_14885"
     CDS             complement(6997..7308)
                     /gene="nirD"
                     /locus_tag="PKX01_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HMZ77467.1"
                     /translation="MSNWKKICAVSDVPVLGSRVVHAEEVGIIAVFRNSADEIFAVRD
                     KCPHKGGPLSQGIVHGSTVTCPLHSWKIQLEDGQVVPPDVGCVKKFPVKVENGEVFLG
                     L"
     gene            complement(7318..>8030)
                     /locus_tag="PKX01_14890"
     CDS             complement(7318..>8030)
                     /locus_tag="PKX01_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HMZ77468.1"
                     /translation="ADELRRIADAVDKYKIPTVKVTGGQRIDLLGVKKEDLPAMWEDF
                     AKAGMCSGHAYGKSLRTVKTCVGAEHCRFGTQRSMDLGVKLEKMLFGMWSPHKVKLAV
                     SGCPRNCAESGIKDVGVIGVDSGYELYIGGNGGIKTEVAQFFCKVTNDDEVMEYGGAF
                     IQLYREEGFYLERTCHYLERVGMEHIKKEVLEDADKRKALYDKLLFALQNYKDPWTEI
                     VEDRSGGEFKRQFEIIKV"
BASE COUNT         1283 a         2407 c         3097 g         1243 t
ORIGIN      
        1 acccgcaacc cgcaacccgc aacccgcaac ccgcaacccg caacccgcaa cccgcgcgcg
       61 acgtgacgtg agatggggcg ccgaggggcc ttgcgccggg gacggcggcc agatgggcga
      121 cctggtccgg tcgcgggacg tgtttcgagg ctcagcccgc gtcggcgcgc acggcgccga
      181 ggagggtgtc gaggatctcg atcatgcgat cgatctcggc ctcggtgacg gtaagggcgg
      241 gcatgaagcg cagcagattg gggcgcggcg cattcaggag caggccttcc ggcgcgtagt
      301 ccagggccgc cttgaccagg gccgggccgc ggtccgaatc gaggatcagg gcgcgcagca
      361 ggcctgcgcc ccgttcgccg gccagggcgt gccgggtgac gagcgcttgc agacgacttt
      421 ccaggtagcg gccgagggtg tcgacccggg ccaggaagcc gtcggagagc aggcgccgca
      481 gcacggccgc gccgacggcg gtcatcaggg gattgccgtt gtaggtgccg ccctggtcgc
      541 cgggctggaa acaacacacg gcctctttgg cgagcagtgc cgacaggggc acgccgccgc
      601 cgatgccctt gccgagggtc atgatgtccg gctcgatgcc ggcatgttcg tgggcgaaga
      661 ggcggcccgt acggcccatg ccggtctgca cctcgtccac gatgaggagc aggccgtggg
      721 cgcgggtgag gtcccgcagg gcttgcagga agccgggttc ggcgggaatg acgccgcctt
      781 cgccctgcac gggttcgagc atgatggcga cggtcttgtc gccgatgagc gccccgacgg
      841 agtccaggtc gttcaggcgg gccttgggaa aacctggcac ctggggcgcg aacagggtgt
      901 cccagccggg cttgccggag gcggacatgg tggcgagggt gcggccgtga aagctgtgct
      961 cgaaggtgat gatctcgtag gcgccgtcct tgtgcatgcg gccccacttg cgggcgagct
     1021 tgatggcgcc ctcgttggct tcggcgccgg tgttggcgaa gaagacccgg tcgaagcagg
     1081 aatgctcggc cagcaggccg gccagttcgg tcatcgggcc gttgaaaaag gccgggctgg
     1141 ggttgatcag gcgcgcggcc tggtcggcga gcgcctctcg gatctcggcc gggcagtggc
     1201 cgaggcagtt gacggcccag ccctggacga agtcgagata gcgcttgccg gtgtcgtcat
     1261 gaagccagga cccgtccccg cgcacgaaga tcagttccgg gcgaggggtg atgaacatca
     1321 gcgacgcagt gtcgaattcg ccgtagtgca tggggttctc ctgagggttg atgccggcag
     1381 gatggcacac gtggccggca ggggtgtgac aggcgcgggg ctcagccgtt cacggacttc
     1441 caggtgcgca cccagcccgg gaattcggcc gccggcatag gatggccgat gccgtagccc
     1501 tggacgcagt cgcagcccag gctcagcagc gggccgcagt gctcgggcgt ttcgacgcct
     1561 tcggcgatca cctgtcggcc gaagctgcgg gcgagggcga caacgccctt gacgatggcc
     1621 agatcctcgg gatcggcgag gatgtcgcgg acgaaggagc ggtcgatctt gagggtgcgg
     1681 gccggcaggc ggcggaaata ggtaagggag gaatagccgg tgccgaagtc gtcgagggcg
     1741 aagctgacgc ccagcgccgt acaggcgcgg atcacgcggg ccacctggtc cacgtcctcc
     1801 atggccgtgg tttcgagaat ctccagctcc agccgctccg gcgcgacgct ggggtgggcg
     1861 atgagctggg cggtgaggtg gtccacgaag tccggtgcca ggaggtggtg gcccgagacg
     1921 ttgatgctga cggggatgtc cagcccggcg gcatgccacc ggctcatctg gtcgagggcc
     1981 gcggcgagga cccagcgatc caggcgcacg cccaccgggc tgctttccag ccagggcagg
     2041 aaggcgtccg gcaggagcag gccttcgtcg gggtgttccc agcggatcag ggcttcggca
     2101 ccgatgacgc tgctcaggcg cagattgacc ttgggctgga agaagaggcg gaactggccc
     2161 ccgtcgagcc cccggtcgac gctgctgacg atgcgctgaa ggtggtgatt gcgctgttcc
     2221 agttccgtgt cgaagagggc gtagcgggcg cggccgcttt gcttggcgag gagcatggcc
     2281 tggtcggcgt ggcgcagcag gacgtcgggt tcttctccgt cgcgggggaa gagggcgacg
     2341 ccggcgctgg cggagatgcg gatctcggcg ccatcctcca ggaccaccgg ctggctgacg
     2401 gttcccagca gacgggccag ggcggccggc atctcctggc ggtcggcgat gccgccgatg
     2461 agcaccagga actcgtcgcc ccccaggcgc gccacggtgt cggaaccgcg caggctgtcg
     2521 cgcaggcgcc ccgccacctg gacgaggatc tcgtcaccca cggtgtggcc ccaggtgtcg
     2581 ttgaagaact tgaagcggtc caggtccagg tagcacaccg ccaccagggt gtcgtcccgc
     2641 gcggcgcggg cgatggcctg acgcaggcgg tcggccagca ggatccggtt gggcagtccg
     2701 gtcagggcgt cgtggtaggc gaggaagcgg atctcctctt cggtgcgtac ctcgtcggtg
     2761 atgtcgcgca gcagggcgta atagccgtcg gtccgtccgt ccgtcgcggt ggcgctgcgc
     2821 agacaggtct cgatccagcg ctcgtcgccg gtctgccggt tgtgccggcg gctgcggaag
     2881 gtggcgctgc cgccgccggc atcgagccgg gcgagattgt gctgctggat gccgtccccg
     2941 agcaccgcat ccacgaggga gccgcgcagg gcggtggggg ccaggccgtg gaagtgggcg
     3001 taattctgat tgacgtagct gtagcagccc cggccgtcga ccgcggcgag catgatgggt
     3061 gtgccctcgg tgagcaggcc gagggtggca ctcaggcggt gcagggcctg gtagcgtgac
     3121 aggtagagcc ggcgcgagtg gagggtcagg aaggcggtga ggagcagcac gttgctcagc
     3181 accatccagc ggctgagggg gggagaggat ccgcccatgg gcagccagtc gtactgttcg
     3241 gagagggaca tgcccagcag ggcgaggatg gacacggccg tcatcagcag ggcgtggcgc
     3301 atgccgaaga gccagccgac gaggacgatg aggaccggga tggccgagag tgaggtggtg
     3361 cggttgccgc ccgtgaaggc tgcgccgatc aggatgatca gccaggcgcc ccacaggacg
     3421 caacgggacg gcaggcggtc gggatggcgg cggaagccga gcaggccggc acagagggca
     3481 atgcagaccc ccagggcggt cagtccgtgg atgggtgaga catcgtgctg ggcgaaaacc
     3541 gacacaccga cgatcagggt caggagcagc gagatgcccc ccatggtgcg cagcagttgc
     3601 cccgggttct cgcggtcgaa cagtggcgga ggaggctgcg aggggtcagg cgtgtgcatc
     3661 atgtcggttc gcgggcgggg gtgtcgggcc ggctcgtgac cgggacaccg cccgctagcg
     3721 taccagcaac agataaatga gcaagatgtt gagggtcagg gaggccgcgg cgaggagctt
     3781 gaggcccagt tgcgggtcgc gctggagcag gggcgacttg cgcggcacga cgagggggcg
     3841 gtgggcgccc cgttccaggg cgaggaggaa ttcctcggcg gtctcgaagc gggcacgggg
     3901 gtcgcgggcg accgccttga ggagcaccgc ctccatccag gcgggaatgt caggccggaa
     3961 gcgggtgggg ggcacaggat cgccgaactt ggggttctgg aagggttcga tctcgccgta
     4021 ggggtacttt cgggtgagca gctcgtacag ggtgacgccg accgcgtaca ggtcggtctg
     4081 ctcgttggcg gcggtcggcg tgggaccgta gagttccggc gccatgtagc tgggcgtgcc
     4141 ggggttgttg atctcgtgga actgctggcc gtcgccgcgg ctgtcggagg cggccacgcc
     4201 caggtcgaga atgcgcaggc ggccgtcgtt gtccaggtgc aggttgtcgg gcttgatgtc
     4261 ccgatggatg atggacaggc gatgcaggcc ggcgagacct ttgaggagcc gttcgcccag
     4321 gctgacgatc tcgcccagcc cgaagcggtg tccgctctcc agtcgggcct tgagggtggc
     4381 gccctcgtgc cagctcatca ggtaatacag gtgggcccgt gccgggtgga gaatcacctg
     4441 gggaaaatag gcgtcgttca cacggcgggc cagccactcc tcgtgggcga gggcggccga
     4501 ggcttccggg tcgttgcgct cggggcgcag ggtcttgagg acccgcggat ggccctgggc
     4561 atcgtggacg cggtagagca gggtggcgat ggattcgtgg agcagggcgt ccacggtgag
     4621 gccgtccagt tcctggccgg gcttgaggcg cggcggcagt gtcagctggc cgaggcgggc
     4681 gatggtgtcg cgcaggcggt cggggggcag ggcgtccaca cgcacgacca gggcggtgca
     4741 gttgtcctgg ctgccgccgt cctcggcttc accggcgagg cgggccgccg cggcctgggg
     4801 ttcgtcctgg ctgcgcagga tgtcggcgat gcgtttgtcg ccgagctggt tccagacgcc
     4861 gtcgctcatc agggcgaaca cgtcgccggg ctccaggtcg ccgtcggcgt agtccaccga
     4921 caggtgaggg tcgaggccga tggcgcggga caggacgttc ttcagttccg ggtgttccca
     4981 caggtggtcg gtggtgagct gctcgaagcg gccgtcgcgc agacggtaga tgcggctgtc
     5041 gcccacgtgg gcggtgtaga agcgcctgcc gcgcaccacg atggctgaca gggtggtggc
     5101 catgccggca gcctcacggg tgcgcgaggc ctgggacacg agccagcggt tgagggcgtt
     5161 gagcaccgtg tcgagggcct tgttgacggc ccaggtgtcg ggggtggcgt agtagtccga
     5221 gagcaaaccg cgcaccgtgt actcggaggc ctcgcgcccg ttggcatggc cgccgactcc
     5281 gtccgccacc acggccagga tgcccttggt gtccagctcg gccccgtcgg gggtgacggc
     5341 gccgcagaag tcctcgttgc gggggcgggg gccggtcaag gaggcgtggc cgatgcgggc
     5401 ctggaggcgg gcgttcttcg gggcggtcgt ggcggcagtc atggaagcgg tccggtgaga
     5461 cgctcagtat gcagaaaagc ccgcccgcca ggcggagcat ggcgggcggg cggagggacg
     5521 tgtgccgcga ccgggcggtt ccccgtggtc cggtgcaagg accgggcggc ggggaacgcc
     5581 gggagggcca actctgcggg gcgtgatccg cgcggtcgca gtgtccgtcg tcgtgctcag
     5641 atctttgcgg aggtgaggtg cggggcgccc caggtggtgc gccagcggct cttcacggcg
     5701 gtgaggccga ccagggcggc gatggccagg gcggcgaaga tcaggaagcc ggactgatag
     5761 ctgccggtga cctgtttgct gtagcccagg ctggaggcca ggtagaagcc ccccacgccg
     5821 ccggccatgc cgaccaggcc ggtcatcacg ccgatttcct tgcggaagcg ctggggcacg
     5881 agctggaaca cggcgccgtt gcccatgccc agggacagca tggcgcagac gaacaccagc
     5941 agggcctgcc aggattcggg cataccgaag ctgacgatgg ccaggaagat ggcggccagc
     6001 acgtacatga cggtcaggct cttgatgccg ccgatgcggt cagccacgtt gccgccgatc
     6061 gggcgcacca gggagccggc gaagacgcag gcggcggtgt agaagccggc ggtcttcgga
     6121 tccagaccgt actgggtgtt gaagtagatg gtcagggacg aggccaggcc gacgaagcca
     6181 ccgaaggtga cgctgtagaa gaacatgaac caccaggcgt ccttgtcctt gagcaccgcg
     6241 aggtattcgg tgagggactt ggcgggcggg cattccggcg catccttggc catgaagagg
     6301 tagagcacga agacgatgga caggggaatc agcgccaggc cgaagacgtt gttccagccg
     6361 taggctgccg ccagaccggg gccgaagagg gcggccaggg cagtaccgga gttgccggcg
     6421 ccggcgatgc ccagggccgt gccctgatgc tccggcggat accagcggga agccaggggc
     6481 agggccacgg caaaggctgc gccggcgacg cccaggaaga agcccagaat cagggtttct
     6541 tcgtagttgt ggatgcccag cttccaggcc gcggcgaggg cgccgatgac gatcacctgg
     6601 ccgatggcgc cggcggtctt gggcttgagg tgatccacca gcacgcccat gacgatgcgc
     6661 agggcggcgc ccgcgagcac cggggtggcg accatcaggc ccttttcggc gtgggacagg
     6721 cccagatcct tggcgatctg cacgcccagg gggccgagca gcacccagac catgaaggcc
     6781 aggtcgaaat agaggaaggc cgcgatcaac gtgggggtgt gaccggcctg gaggaaggat
     6841 ttacgatcca ttttgggact cctggggatg agagggcccg gacggcggtc cagaagggac
     6901 cccgacgggg attcaacatt aaaaatcggg gaggctcacg ccgcggaggc gacgggggag
     6961 acggccgcag ccgacgtcgc ccccggagcg cgaaccttac aggccgagga aaacctcgcc
     7021 gttctcgacc ttgacgggaa acttcttcac gcagcccacg tccggcggca ccacctggcc
     7081 gtcctccagc tggatcttcc agctgtgcag ggggcaggtg acggtgctgc cgtggacgat
     7141 gccctgggac aggggaccgc ccttgtgggg gcacttgtcg cgcaccgcga agatctcgtc
     7201 ggcgctgttg cggaagacgg cgatgatgcc gacctcttcc gcatgcacga cgcgggagcc
     7261 caggacgggg acgtcactga cggcgcagat ttttttccag ttgctcatga cggaccctta
     7321 taccttgatg atttcgaatt ggcgcttgaa ctcgccgccg gaacggtcct cgacgatctc
     7381 ggtccagggg tccttgtagt tctgcagggc gaagagcaac ttgtcgtaga gggccttgcg
     7441 cttgtcggcg tcttccagga cttctttctt gatgtgctcc atgccgaccc gctccaggta
     7501 gtggcaggtc cgttccagat agaagccttc ttcccggtag agctggatga aggcaccgcc
     7561 gtattccatg acctcgtcgt cgttggtgac cttgcagaag aactgcgcca cctcggtctt
     7621 gatgccgccg ttgccgccga tgtagagctc gtagccggaa tcgacgccga tcacacccac
     7681 gtccttgatg cccgactccg cgcagttgcg cgggcagccg gacaccgcca gcttgacctt
     7741 gtgggggctc cacatgccga acagcatctt ttcgagcttg acgcccaggt ccatggagcg
     7801 ctgggtgccg aagcggcagt gctcggcgcc gacgcaggtc ttcacggtgc gcaggctctt
     7861 gccgtaggcg tggccggagc acatgccggc cttggcgaag tcttcccaca tcgccggcag
     7921 gtcttccttc ttcacgccga gcaggtcgat gcgctggccg ccggtgacct tgacggtcgg
     7981 gatcttgtac ttgtcgaccg cgtcggcgat gcggcgcagc tcgtcggcgg
//
LOCUS       DAOQPA010000139         7854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5128_length_7854_cov_13.156046, whole genome shotgun sequence.
ACCESSION   DAOQPA010000139 DAOQPA010000000
VERSION     DAOQPA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5128_length_7854_cov_13.156046"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="tal"
                     /locus_tag="PKX01_14895"
     CDS             complement(<1..977)
                     /gene="tal"
                     /locus_tag="PKX01_14895"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HMZ77469.1"
                     /translation="MNPIQAARAAGQQIWLDNLSRALINEGTLARHIADGVAGVTTNP
                     AIFHKAIAEGLDYLTDLDALRARPLDAEARYEALVIQDVQRACDLMRPVFDASQGDAG
                     YVSLEVSPALADDEAGTVAAARRLAAAVARDNLLVKIPATAAGVHAIETLTGEGLSIN
                     VTLMFSPAHVEAVAAAYVRGLEALAAKGGDLSKVRSVASVFLSRWDSLVDKQLEELGS
                     EAALALRGRTGVAFARRAYQQYLARFHGDAFATLAARGARPQSMLWASTGTKNAAYSD
                     LLYVEALIGPETVNTLPDATLAAFLDHGRVTPGAVTAGVADADAHIAALA"
     gene            complement(1025..1780)
                     /gene="gspN"
                     /locus_tag="PKX01_14900"
     CDS             complement(1025..1780)
                     /gene="gspN"
                     /locus_tag="PKX01_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HMZ77470.1"
                     /translation="MRKFALFLLIFLVLALMRAPATLLDAAFSHTTGAGLRLLLADGS
                     LWAGRGTLASSDPGGRSVTPWLPLAWQFEPTALLQGALRWRFTSDTRPVATVDLGLGG
                     ASVSELDLHTPANALLASIPHPIARAGWQGDLDLGASHWACAADGRCSGEARLLWRGA
                     RSALFPGRRFGDYEVRIQADRGQLQFTVATLEGEVRLDGRGSAAPGQPPRFDGQISGD
                     ADFLSRLPAIAGGAATPTPQAGRYTLHWPPGSR"
     gene            complement(1786..2295)
                     /gene="gspM"
                     /locus_tag="PKX01_14905"
     CDS             complement(1786..2295)
                     /gene="gspM"
                     /locus_tag="PKX01_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HMZ77471.1"
                     /translation="MNAPIAAARVTPLARLLTAWNACSQRERRLILIAGSVVALGLVF
                     GLADWSAQERRRLDRQVPRAQAQLEQTQEAATEIAALRGRPPVQPLAGPALADAVGAA
                     AKARGLAVTVNSTGDGVQIRGNGDFDALVGWLGTLHSDQGLRVLRLEIQRAGSQASVD
                     ALLAGPAAP"
     gene            complement(2292..3512)
                     /gene="gspL"
                     /locus_tag="PKX01_14910"
     CDS             complement(2292..3512)
                     /gene="gspL"
                     /locus_tag="PKX01_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HMZ77472.1"
                     /translation="MTTRLLLALDEHWPAEPACPWVLLGPDGQPTAEGHSEPRHWPAA
                     AEVEVVLTGAQCLWLEVPLPGAARRDLPRLIAYALEDRLLKDPDTQHLTVSHRRPAGD
                     GERDLVGVLVIARERLRSLLAQLAALGRSPRRVVAAVQTAPAGPEGWHLAVAGPDAVL
                     RSAVTTGVALDADLLIPLLAQQAATARAANQAPDALHVHLAPGSGGPDTTALARETGL
                     PVSVAEPYRWWAGADRHAASLLHGEFASREQGAAWLARLRPAALLAAAALGVWLLANV
                     GEVLWLRHSLDGVQTRIARVYATTFPNTPVVAPAAQMRQQLNQERARHSLLRDDDALA
                     LLAQAADALGTDALDGVAALRYEDGRLDLSLQGPAAARAPALGGLLAARGLLADVRRD
                     GNATHLVLRRESLQ"
     gene            complement(3509..4474)
                     /gene="gspK"
                     /locus_tag="PKX01_14915"
     CDS             complement(3509..4474)
                     /gene="gspK"
                     /locus_tag="PKX01_14915"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HMZ77473.1"
                     /translation="MSRRPVAPAVAPSSRPLQRGAAVILALLTVALVAGLAAAAVGDL
                     GVALDSVAGRHDQAQARQLARGAVDWARNVLAEDARSTSVDHLGESWAVRVPPTPVEE
                     GEVSGELQDLSGRLNLNNLIRADGASVEDVAAFRRLLGLVGVGDADAIALSSALLDWL
                     DADDLPRLPGGAESAWYGSQDPPRRAANARLAAVAELKQIRGFTPDLVARLTPFVAAL
                     PPDTTVNVNTAPAEVIAAVVPGLGLDGARQVVAQRARAWFKDVADFTARLPAGNASPD
                     TSRIATTSRHFLATGRARFGVSTVRMEVLLDRSQKWPDIVWQKLL"
     gene            complement(4471..5040)
                     /gene="gspJ"
                     /locus_tag="PKX01_14920"
     CDS             complement(4471..5040)
                     /gene="gspJ"
                     /locus_tag="PKX01_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HMZ77474.1"
                     /translation="MKRFHARGLTLIELMVALAIFAVLGVLSYRALAEVSTSRARLEE
                     NFQRWRNISRAVQRIDADLLQIAAPGGQNGVARSPALTVLRSAGGGSELQFLRLDDTR
                     GIRRYGFRAEDNRLVWLRWSGRDPADAPAVEPLLDNVREVRWHFLKDNLRSDAWPPDA
                     NQPDVLPDAIIVEIDLPDAGTLTRWIALR"
     gene            complement(5037..5411)
                     /gene="gspI"
                     /locus_tag="PKX01_14925"
     CDS             complement(5037..5411)
                     /gene="gspI"
                     /locus_tag="PKX01_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HMZ77475.1"
                     /translation="MKPAPRGFTLLETLVALTILAIALTAAFRALGVATQSSAELRDR
                     LLGDWIAENRLAELRATHVWPEPGSNEGQAVQAGRSYRWREEIRGTPNPLFRRADISV
                     YSEDSGDHAIARLSGYVVKPLR"
     gene            complement(5408..5977)
                     /locus_tag="PKX01_14930"
     CDS             complement(5408..5977)
                     /locus_tag="PKX01_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ77476.1"
                     /translation="MSAAHPFPIPTPGLPPARHARRADAFTPRPPRCAAGFTLIEVMV
                     VLVIMGLMATGISVGLDSLRGREADQALRRLRLVLEASADRAAVRGRPLAIEFLPDGY
                     RFAALDADDTWRPLIDPPVFTERTLPEGLSWSGLRLDGQDAPQPRLQFGTQAPEYELR
                     VATPRGIALLTGKANGDVLLTPPDAPPRS"
     gene            complement(5974..6402)
                     /gene="gspG"
                     /locus_tag="PKX01_14935"
     CDS             complement(5974..6402)
                     /gene="gspG"
                     /locus_tag="PKX01_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HMZ77477.1"
                     /translation="MSQGPRRARGFTLIEVMVVIVILGVLAALVVPKVMSRPDEARVV
                     AARQDISAIMQALKLYKLDNRRYPTAEQGLQALVVKPGSAPVPENWKSYLEKLPNDPW
                     GKPYQYVIPGLKGEVDVMSFGADGVQGGEGFDADIGSWSL"
     gene            complement(6413..7615)
                     /gene="gspF"
                     /locus_tag="PKX01_14940"
     CDS             complement(6413..7615)
                     /gene="gspF"
                     /locus_tag="PKX01_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HMZ77478.1"
                     /translation="MTAFRYRALDAAGKETAGVLEADTGKAARGLLRERGLFPLEVAS
                     VSQGGGARSARRRLKDADLTLLTRQWATLLASGLTVEQALAALIEQAETDATRQLLAG
                     VRSEILAGYSLRAALDRYPQAFSTLYRASIAAGEKSGELARVMNQLADYLERSNALRT
                     KTLQALLYPAIVAGVALMVIVGLMTYVVPQVVSVFQQSKQALPLLTRALIAASAVLRD
                     WGWLMVLALIGAFAAGRVALREPHLKRRWDAWLLRLPVLGRHLRTLDATRFASTLSIL
                     AGSGVPLLAALDAGRQVIARLPLADAVATATERVREGQPLSRALGTSRQFPPLLIHML
                     ANGEATGRVDELLDRAARLQQAELENRTAALTALLEPVLLLLMGGFVLLIVMAVMQPI
                     IEINTLMK"
     gene            complement(7635..>7854)
                     /locus_tag="PKX01_14945"
     CDS             complement(7635..>7854)
                     /locus_tag="PKX01_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HMZ77479.1"
                     /translation="ECARSGYAGRTGLYELMVADEALQRLIHTAADEAALRAHARAQG
                     TRSLRDDGLRLLADGTTSAEELLRVTRD"
BASE COUNT         1030 a         2336 c         3433 g         1055 t
ORIGIN      
        1 gccagggcgg cgatgtgggc gtcggcgtcg gccacgcctg cggtgactgc gccgggggtg
       61 acgcggccgt ggtcgaggaa ggcggcaagg gtggcgtcgg gcagggtgtt caccgtttcc
      121 gggccgatga gggcttccac gtagagcaga tcggaatagg cggcattctt ggtgccggtg
      181 ctggcccaca gcatgctctg cggtcgggcg ccacgggcgg cgagggtggc gaaggcgtcg
      241 ccgtggaagc gcgccaggta ttgctggtag gcgcggcggg cgaaggcgac gccggtgcgg
      301 ccgcgcaggg cgagggcggc ctcgctgccg agttcttcga gctgcttgtc caccaggctg
      361 tcccagcggg acaggaagac gctggccacc gagcgcacct tggagagatc gccgcccttg
      421 gcggcgaggg cttccaggcc gcgcacgtag gcggcggcga cggcctccac gtgggcgggg
      481 gagaacatca gggtgacgtt gatggagagg ccttcgccgg tgagggtttc gatggcgtgc
      541 acgccggcgg cggtggcggg gatcttgacc agcaggttgt cgcgggcgac ggcggcggcg
      601 aggcggcggg cggcggccac ggtgccggct tcgtcgtcgg cgagggcggg ggagacttcc
      661 aggctcacgt agccggcgtc gccctggctg gcgtcgaaga ccgggcgcat caggtcgcag
      721 gcgcgctgca cgtcctggat gacaagggct tcgtagcggg cttcggcgtc gagggggcgg
      781 gcgcgcaggg cgtcgagatc ggtgaggtag tcgagccctt cggcgatggc cttgtggaag
      841 atggccgggt tggtggtgac gccggccacg ccgtcggcga tgtggcgggc cagggtgcct
      901 tcattgatga gggcgcggga caggttgtcg agccagatct gctggccggc ggcgcgggcg
      961 gcttgaatcg gattcatggg cggtggaaat tcggagggac gatgaatgga attgaccggg
     1021 cattctaacg gctgccgggg ggccagtgga gggtgtagcg accggcttgc ggcgtgggcg
     1081 tggcagcccc gccggcgatg gcgggcaggc gcgacagaaa atccgcgtcg ccgctgatct
     1141 ggccgtcgaa acggggcggc tgacccggtg ccgcgctgcc gcgaccgtcc aggcggacct
     1201 cgccttcgag ggtggcgacg gtgaattgga gctggccccg gtcggcctgg atgcggactt
     1261 cgtaatcgcc gaagcggcgg ccggggaaca gggcactgcg ggcaccgcgc cacaacaggc
     1321 gggcctcgcc gctgcagcgg ccgtctgccg cacaggccca gtgggacgcg ccgagatcca
     1381 ggtcgccctg ccagccggcg cgggcgatgg ggtggggaat ggaggcgagc agggcgttgg
     1441 cgggggtgtg cagatcgagt tccgagacgc tcgcgccgcc gaggcccagg tccacggtgg
     1501 ccaccgggcg ggtgtcgctg gtgaagcgcc agcgcagggc gccctggagg agggcggtgg
     1561 gttcgaactg ccaggccagg ggcagccacg gggtgacgga gcgaccgccc ggatcgctgc
     1621 tggccagcgt gccgcggccg gcccacaggc tgccgtcggc gagcagcagg cgcaggccgg
     1681 cgcctgtggt gtggctgaag gcggcgtcga gcagggtggc cggcgcgcgc atcagcgcca
     1741 gcacgagaaa gatcagcagg aacagggcga acttgcgcac ggggatcagg gcgcggcggg
     1801 gccggcgagg agggcgtcca cgctggcctg actgccggcg cgctggatct cgagacgcag
     1861 cacgcgcagg ccctgatcgc tgtggagggt gccgagccag ccgacgaggg cgtcgaaatc
     1921 gccgttgccg cggatctgta cgccgtcgcc ggtgctgttc acggtgaccg ccaggccgcg
     1981 ggccttggcg gcggcgccca cggcgtcggc cagggctggg ccggcgaggg gctggacggg
     2041 cgggcggccg cgcagggcgg cgatctcggt ggcggcttcc tgggtctgct ccagctgggc
     2101 ctgggcgcgg ggcacttgcc ggtcgaggcg ccggcgttcc tgggcggacc agtcggccag
     2161 gccgaagacc aggccgaggg cgaccacgct gccggcgatc aggatcagac ggcgctcccg
     2221 ttgggagcag gcgttccagg cggtgagcag gcgcgcgagg ggggtgacgc gggcggcggc
     2281 gatgggggcg ttcattgcag gctctcccgg cgcaggacga ggtgggtggc gttgccgtcg
     2341 cggcgcacgt cggcgagcag gccgcgggcg gcgaggaggc cgccgagggc cggcgcgcgg
     2401 gcggcggcag ggccttgcag gctcaggtcg aggcggccgt cttcgtagcg cagggcggcg
     2461 acgccgtcga gggcgtcggt gccgagggcg tcggcggcct gggcgaggag ggcgagggcg
     2521 tcgtcgtccc gcagcaggct gtggcgcgcc cgttcctggt tgagctgctg acgcatctgg
     2581 gcggcggggg cgacgaccgg ggtgttgggg aaggtggtgg catacacccg cgcgatgcgc
     2641 gtctgcacgc cgtcgaggct gtggcgcagc cacaggactt cgcccacgtt ggcgaggagc
     2701 cacacgccca gcgcggcggc ggcgaggagg gcggcgggac gcaggcgggc gagccacgcg
     2761 gcgccctgct cgcgggaggc gaattcgccg tggagcaggg aggctgcgtg gcggtcggcg
     2821 ccggcccacc agcggtaggg ttcggcgacg ctcaccggca gtccggtctc gcgggccagc
     2881 gcggtggtgt cggggccgcc gctgccgggc gcgagatgca catgcagggc atcgggcgcc
     2941 tgattggcag cgcgggcggt ggcggcctgc tgggcgagga gggggatgag gaggtcggcg
     3001 tcgagggcga ccccggtggt gaccgcactg cgcagcacgg cgtccggccc ggccacggcc
     3061 aggtgccagc cttccgggcc ggcgggggcg gtctgcacgg cggcgacgac ccgtcgcggg
     3121 ctgcggccga gggcggcaag ctgggccagc aggctgcgca ggcgctcgcg ggcgatcacc
     3181 agcacgccga cgagatcccg ttcgccgtcg ccggcggggc ggcggtggct gacggtgagg
     3241 tgctgggtgt ccgggtcctt gaggaggcgg tcctccaggg cataggcgat gaggcgcggc
     3301 agatcgcgac gggcggcgcc gggcaggggg acttccagcc acaggcactg ggcgccggtg
     3361 aggacaactt cgacctcggc ggcggcgggc cagtggcggg gttcggaatg gccttcggcg
     3421 gtgggctggc cgtcggggcc gaggagcacc caggggcagg cgggttcggc cggccagtgc
     3481 tcgtcgaggg caaggaggag gcgggtggtc atagcagttt ctgccaaacg atgtcgggcc
     3541 acttctggct gcggtcgagg agcacttcca tgcgcacggt ggagacgccg aagcgggccc
     3601 ggccggtggc gaggaagtgg cggctggtgg tggcgatgcg ggaagtatcg gggctggcgt
     3661 tgccagcggg aaggcgtgcg gtgaagtcgg ccacgtcctt gaaccaggcg cgggcgcgct
     3721 gggcgaccac ctggcgggcg ccgtcgaggc cgaggccggg cacgacggcg gcgatgacct
     3781 cggcgggggc ggtgtttaca ttgacggtgg tgtcgggcgg cagggcggca acaaagggcg
     3841 tcaggcgcgc caccagatcg ggcgtgaagc cgcggatctg cttgagttcg gcgacggcgg
     3901 cgaggcgcgc gttggcggcg cgacgcggcg ggtcttggct gccataccag gcggattcgg
     3961 cgccgccggg caggcgcggc aggtcgtccg cgtcgagcca gtcgaggagg gccgagctga
     4021 gggcgatggc gtccgcgtcg ccgacgccga cgaggcccag caggcggcgg aaggcggcca
     4081 cgtcttcgac ggaggcgccg tcggcgcgga tcaggttgtt gaggttgagg cggccggaga
     4141 gatcctgcag ttcgccgctg acttcgcctt cttccaccgg cgtggggggc acgcgcacgg
     4201 cccaggattc gcccaggtgg tccacgctgg tgctgcgggc gtcctcggcg aggacgttgc
     4261 gggcccagtc gacggcgccg cgggcgagct ggcgggcctg ggcctggtcg tgacggccgg
     4321 cgacgctgtc cagggccacg cccaggtcgc ccaccgcggc ggcggcgagg ccggccacca
     4381 gggccacggt gaggagggcg aggatcaccg cggcgccgcg ctggaggggg cgggacgatg
     4441 gggcgacggc gggagcgacg gggcggcggc tcatcgcagg gcgatccagc gggtgagggt
     4501 gccggcgtcg ggcaggtcga tctcgacgat gatggcgtcg ggcaggacat ccggctggtt
     4561 ggcgtcgggc ggccaggcgt cgctgcggag gttgtccttg aggaagtgcc agcgtacctc
     4621 gcgcacgttg tcgaggaggg gctcgacggc gggggcgtcg gccggatcgc gaccgctcca
     4681 gcgcagccac acgaggcggt tgtcctcggc gcggaagccg tagcggcgga tgccgcgggt
     4741 gtcgtcgagg cgcaggaact ggagctcgct gccgccgccg gcagaacgca gcacggtcag
     4801 cgcggggctg cgggcgacgc cgttctggcc gccgggggcg gcgatctgca gcaggtcggc
     4861 gtcgatgcgc tgaacggcgc ggctgatgtt gcgccagcgc tggaagttct cttccaggcg
     4921 ggcgcggctg gtgctgacct cggcgagggc gcggtaggag agcacgccca gcacggcgaa
     4981 gatggccagg gcgaccatca gttcgatgag ggtgagcccg cgggcgtgga agcgcttcat
     5041 cgcaggggct tgaccacgta gccggacagc cgcgcgatgg cgtggtcgcc ggagtcttcg
     5101 ctgtacacgc tgatgtcggc gcggcggaac aggggattgg gcgtgccgcg gatctcttcg
     5161 cgccagcggt agctgcgtcc ggcctgcacg gcctggcctt cgttgctgcc gggttcgggc
     5221 cacacgtggg tggcgcgcag ctcggcgaga cggttttcgg cgatccagtc gcccagcaga
     5281 cggtcgcgca gctcggcgga ggattgggtg gcgacgccca gggcacggaa ggcggcggtc
     5341 agggcgatgg ccaggatggt cagggcgacg agggtttcca gcagcgtgaa gccgcgcggg
     5401 gcgggcttca tgagcgggga ggcgcgtcgg gtggggtgag gaggacgtcg ccgttggctt
     5461 tgccggtgag cagggcgatg ccgcgggggg tggcgacgcg cagttcgtat tccggggcct
     5521 gggtaccgaa ctgcaggcgc ggttgaggcg cgtcctggcc gtccaggcgc aggccggacc
     5581 agctgaggcc ttcgggcagg gtgcgttcgg tgaacaccgg cgggtcgatg agggggcgcc
     5641 aggtgtcgtc ggcgtccagc gcggcgaaac ggtagccgtc aggcaggaat tcgatggcga
     5701 gggggcgtcc gcgcaccgcg gcccggtcgg cgctggcttc cagcaccagg cgcaggcggc
     5761 gcagggcctg gtccgcctcg cgcccgcgca ggctgtcgag gccgacgctg atgccggtgg
     5821 ccatgaggcc catgatgacc agcacgacca tcacttcgat gagggtgaag ccggcggcgc
     5881 agcgtggcgg ccggggcgtg aacgcgtccg cgcgccgggc gtgccgcgct ggaggcaggc
     5941 cgggcgtcgg gatcgggaaa gggtgtgccg cgctcacaga ctccacgagc cgatgtcggc
     6001 gtcgaagcct tcgcccccct gcacgccgtc ggcgccgaag ctcatcacgt ccacctcgcc
     6061 cttgaggccg gggatgacgt actggtaggg cttgccccag gggtcgttgg ggagcttttc
     6121 cagataggac ttccagttct ccggcaccgg ggcgctgccg ggcttgacca ccagggcctg
     6181 gaggccttgt tcggcggtgg ggtagcggcg gttgtcgagc ttgtagagct tgagggcctg
     6241 catgatggcg ctgatgtcct ggcgggcggc gacgacgcgg gcttcgtccg ggcggctcat
     6301 gaccttgggc accaccaggg cggcgaggac gccgaggatg acgatgacga ccatcacttc
     6361 gatcagggtg aagccgcggg cgcggcgcgg accttgggac atggcgggtt ccttatttca
     6421 tcagggtgtt gatctcgatg atgggctgca tgacggccat cacgatgagg agcacgaagc
     6481 cgcccatgag gaggagcagc acgggctcca gcagggcggt gagggcggcg gtgcggtttt
     6541 ccagttcggc ctgctggagg cgggcggcgc ggtcgaggag ttcgtccacc cggccggtgg
     6601 cttcgccgtt ggccagcatg tggatgagca ggggcggaaa ctggcggctg gtgccgaggg
     6661 cgcgggacag gggctggcct tcgcggacgc gttcggtggc ggtggcgacg gcgtcggcca
     6721 ggggcaggcg ggcgatgacc tggcggccgg cgtcgagggc ggcgaggagg ggcacgccgc
     6781 tgccggcgag gatggacagg gtgctggcga agcgggtcgc gtcaagggtg cgcaggtgac
     6841 ggccgaggac cggcaggcgc agcagccagg cgtcccagcg gcgcttgagg tggggttcgc
     6901 gcagggccac gcggccggcg gcgaaggcgc cgatgagggc aaggaccatc agccagcccc
     6961 agtcccgcag cacggcgctg gcggcgatga gggcgcgggt gaggaggggc agggcctgct
     7021 tgctctgctg gaagacggag acgacctggg gcaccacgta ggtcatcagg ccgacgatga
     7081 ccatcagcgc cacgccggcc acgatggcgg ggtagagcag ggcctgcagg gtcttggtgc
     7141 gcagggcgtt gctgcgttcc aggtagtcgg cgagctggtt catgacccgc gccagttcgc
     7201 cggatttttc gccggccgcg atggaggcgc ggtagagggt ggagaaggcc tggggatagc
     7261 ggtcgagggc ggcgcgcagg ctgtagccgg cgaggatctc ggaacgcacg ccggcgagga
     7321 gctggcgggt ggcgtcggtt tcggcctgtt cgatgagggc ggcgagggcc tgttcgacgg
     7381 tgaggccgga ggcgagcagg gtggcccact ggcgggtgag cagggtgagg tcggcgtcct
     7441 tgaggcgccg ccgggcactg cgggcgccgc caccctggct gacgctggcg acttcgaggg
     7501 ggaacaggcc gcgctcgcgc agcaggccgc gggcggcctt gccggtgtcg gcttcgagga
     7561 cgccggcggt ctccttgccg gcggcgtcga gggcgcggta gcggaaggcg gtcatggggc
     7621 ggggccgggc gggttcagtc gcgggtcacg cgcaggagtt cctcggccga ggtggtgccg
     7681 tcggcgagga ggcgcaggcc gtcgtcgcga aggctgcggg tgccctgggc gcgggcgtgg
     7741 gcgcgtagcg cggcttcgtc ggcggcggtg tggatgaggc gctgcagggc ctcgtcggcg
     7801 accatcagct catagaggcc ggtgcgcccg gcgtagccgc tgcgggcaca ttcg
//
LOCUS       DAOQPA010000140         7804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5167_length_7804_cov_15.710930, whole genome shotgun sequence.
ACCESSION   DAOQPA010000140 DAOQPA010000000
VERSION     DAOQPA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_7804_cov_15.710930"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKX01_14950"
     CDS             <1..199
                     /locus_tag="PKX01_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77480.1"
                     /translation="RPWLHVTQGFPTGMHDDRGGTIAYFLGTRGFLNPTIASFLGSTA
                     YFFGNSGFSRPMIAYFLGTIA"
     gene            776..3010
                     /locus_tag="PKX01_14955"
     CDS             776..3010
                     /locus_tag="PKX01_14955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ77481.1"
                     /translation="MTALPFQPRRLALLLLSSCPALLATVPARAAEGPAALPEVEVKA
                     TRLRPLAEGAGPTSARLAGLKATTSDTAALLQDVPGVALAGAGGLSSLPVVRGLADDR
                     NRVQVDGMDLISACGNHMNPPLSYLESFRVGAIQVFTGAVPVSLGGDSIGATVVAESA
                     PPPFAAPGQGTVAGGALGAFYRSNGAARGVQGEAHWASERLSVRYAGGVSTSDNLRAA
                     KDFKPATQATWTRSGTHPLDANEIGSTAYTLRSHSLGFALRSGEHMADLKVSVQEIPR
                     QGFPNQHMDMTDNHSTQVNLGYTGSYAWGSLQARLYNEETRHRMDFGDDKLYWYGSAR
                     NVAGMPMETHGVNTGARVRADVNLNERDILRVGSEYQRYHLNDWWPPVANAMMMSPNT
                     FQNLHNGQRDRMDVFAEWEAAWSPRWQTLAGLRSSTVRMDADPVQGYNTMAMMYGADA
                     ARFNAASRARTDHNLDATLLARFTPDAGRSFDAGYARKTRSPSLYERYTWSTNGMAMT
                     MNNWVNDGNGYVGSIDLAPEVAHTLSASADLHDAGQARWGVKLQSFYTYVNNYINARC
                     LTSCPPNQFNYLRLVNTDARLYGADLSGYRVLDGGETLGPITGRGTLAYVHGKDTHSD
                     DGLYNQMPLNARLSLEQKHGPWTTAVEEVLVGAKRAVSQVRNEIATGGYALLNLRSSY
                     DTRHYRVDLGLENALNRFYASPLGGAYIGQGTTMSLNGAGAPYGIAVPSMGRSVYVAL
                     TVRY"
     gene            complement(3123..3467)
                     /locus_tag="PKX01_14960"
     CDS             complement(3123..3467)
                     /locus_tag="PKX01_14960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ77482.1"
                     /translation="MSEVTTASGLVIEDLEVGTGPEASAGQRVQVHYTGWLTDGRKFD
                     SSKDRNDPFVFPLGARHVIAGWDEGVQGMKVGGRRKLTIPPNLGYGARGAGGVIPPNA
                     TLVFEVELLAVA"
     gene            complement(3504..4046)
                     /gene="msrA"
                     /locus_tag="PKX01_14965"
     CDS             complement(3504..4046)
                     /gene="msrA"
                     /locus_tag="PKX01_14965"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ77483.1"
                     /translation="MTDTTSETAILAGGCFWCLEAVFLLAEGVHTVTSGYIGGHVDAP
                     TYHQVCDGTTGHAEAIRIEFDPARITFDDLLEIFFAIHDPTTLNRQGNDVGTQYRSAI
                     FATTPAQAAAAQARIAALAGAYHAPIVTEVVPAPVFWPAEPYHHDYFSRNGHQPYCQV
                     IVAPKVQKFLRAFPQRVRST"
     gene            complement(4071..4796)
                     /locus_tag="PKX01_14970"
     CDS             complement(4071..4796)
                     /locus_tag="PKX01_14970"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HMZ77484.1"
                     /translation="MTEPLQPCRIPVSAVWAALAEGGRDFRRHPGPAALFASVFAALG
                     LGLFGVLAAYDLHALRLPLAGGFLLVGPALLAGFFAVRRVAATRPPRLADLLGGFAAA
                     PRGVWALAAVCALLFLIWMSDAATVYSFMIGDTRPGADQVWRFHAWTSLMGGVFALIV
                     FHITAFAVPLLLDRRATLVGAVVASVRGIWTNGPAMATWAGVLAVAIIATVFVPPLLG
                     VSLPWLAFVSDLLYLRVYPSRPK"
     gene            complement(4902..>7804)
                     /locus_tag="PKX01_14975"
     CDS             complement(4902..>7804)
                     /locus_tag="PKX01_14975"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMZ77485.1"
                     /translation="IPFRPIKERMDMLEEGVQVVRLLTTQERSNFEGNYYRLENAACK
                     PRPVQARPRIWIGGNGEKRTLRIAARHADGAVTVQELLARAGKARLSVRGAADLAGDG
                     RFELKGRLDHFDPHAFFTEAPAADLTLDLEARGRRSPQMAATLSFQLAPSTLEGRPLV
                     GHGTLAVDGRRLSDADLTLAVAANRLSARGRYGAPGDALDLHLEAPDLAALGHTLSGR
                     VTAAAVLRGTPARPAGELELSGEGLAYGTVHVEGVNARGRLEEGADAPLNLLLNLAGV
                     RTGADLEHGLRRASLVVDGRRSAHRAHLEAHTPGADALELDLSGGLGDALRWQGRLDR
                     LRVLGPHATTLAAPVPLVLAPAEVRIGAAELRSEAGAALRLDETRWADGEIVARGRLS
                     GLPVGIGLDAAGANRAALGDTLRMGGDWDIHVGGHVDGTVHLFRERGDLLVLGDAPVR
                     LGLDEVQIVVNAVASRLAFGFSVSGAQLGKASGAGTALAERLADGGVRLVPDAPLLGS
                     AQLDMPSIAWTGPLADPNLKTAGRLKADFTLSGTPAQPVTEGRIRGEALAFAMADQGL
                     NLSGGTLVADFNRDRLRVERFDFASPNRVKPNEGRIDVARLTRTPGSLQVKGEVALAS
                     GAGAFSFQAERLPLLQRPDQWLVLSGAGEVHSGWNSARIRGRLTADAGYVELSRTPAP
                     SLGDDVEVVRPEQARPPAVKPFSTAVDLAIGLGDALYVRALGLDTRLAGELRLRAGDG
                     QPLAMTGVINTQGGVYEGYGQKLDIERGIVTFTGAVSNPSLNVVALRKGLSVEAGVAI
                     TGTARRPLVRLVSEPNVPDPEKLAWIVLGRPPDQGSGGELALLLPAAQALLGTDSMSS
                     GLVRALGFDEFSFGSSVDSRQRIQTSSVATGSVTGTTSSAGTTTGSESTAGQVVSIGK
                     RLSARALLSFEQSVTGASSIVKLSYQLTRRIAVVGRAGSENALDAVFTLSFR"
BASE COUNT         1216 a         2667 c         2814 g         1107 t
ORIGIN      
        1 gcgcccctgg cttcacgtca cgcaaggctt cccgaccggc atgcatgacg accgcggagg
       61 gacgattgcc tattttttag gcacgcgagg ctttctgaat cccacgattg cctcattttt
      121 aggcagcact gcctattttt ttggcaacag tgggttttcg agacccatga ttgcctattt
      181 tttaggcacg attgcctaaa ttttaggcaa cgctgcctct tttttaggcg gcattgccta
      241 ttttttaggc agcgtcgcct cataaaaagg caatcatgag tctctggaag ccacgcgtgc
      301 ctctttttga ggcaatcgag ggtcgacacg caccgagtgg agaggcggcc cccgtgagtg
      361 tccaatcgtg tgtgtgtcag gcccggtaaa gccccatccc ggtcaatagc gagacgggct
      421 gctccgggtt cgggatcacc ggtctgccgc gctcgccgtc cggcaagacg gcgggccggg
      481 aacgcgtccg gaacggtcgc aggtgcgggg gtggccgcgt gtggcagtca ccgccggccg
      541 aggcgtacgt ggcagccggc acgcggcgcg aggggggcgg acccggcgac gggccacaca
      601 ccataccggt tgtgggttcg acactgtctc acggggcgca cgtgagggca gacctgctgg
      661 gcaggcgccc gcgcagcctc ggcaaggcgc cgtcgatgcg acgataggcc gcaggggtgc
      721 gcccaggcga tccgggacaa tttcggcttc gtccctgacc tgcttcggat ctgccatgac
      781 cgccctgcct tttcagcccc gccggctcgc cctgctcctt ctttcgtcct gtcccgccct
      841 gctggcgacc gtgccggcac gcgcggcaga aggccccgcc gctctgcccg aggtggaggt
      901 caaggccacc cgtttgcgtc ccctcgcgga gggggcgggg cccacgtccg cgcggctggc
      961 cgggctgaag gccaccacca gcgacacggc cgccctgttg caggacgtgc cgggtgtcgc
     1021 cctcgccggc gccggcggtc tctccagtct accggtggtg cgcggcctgg cggatgaccg
     1081 caaccgggtt caggtggacg gcatggacct gatctccgcc tgcggcaatc acatgaatcc
     1141 ccccctgtcc tacctggagt ccttccgtgt gggggcgatc caggttttta ccggcgcggt
     1201 accggtgagc ctgggcggcg acagcatcgg cgccaccgtg gtggcggaat cggcaccgcc
     1261 gcccttcgcc gcaccgggcc aaggcaccgt ggcgggcggc gcgctgggcg ccttctaccg
     1321 ttccaacggc gcagcccgcg gcgtgcaggg cgaggcccac tgggccagcg agcgactcag
     1381 cgtgcgctat gcggggggcg tgagcacctc ggacaacctg cgtgccgcca aggacttcaa
     1441 gcccgccacc caggctacgt ggacccgcag cggtacccac cccctcgacg ccaacgagat
     1501 cggctcgacg gcgtacaccc tgcgcagcca cagcctgggc ttcgccctgc gcagcggcga
     1561 gcacatggcc gacctgaagg tgagcgtgca ggagattccc cgccagggct tccccaacca
     1621 gcacatggac atgacggaca accacagcac ccaggtcaac ctgggctaca ccggcagcta
     1681 tgcctggggc agcctgcagg cgcggcttta caacgaggaa acgcgccacc ggatggattt
     1741 cggcgacgac aaactgtact ggtatggcag cgcccgcaac gtggccggca tgcccatgga
     1801 gacccatggc gtgaataccg gtgcccgcgt ccgcgcggat gtaaacctca acgagcggga
     1861 tattctgcgc gtcggcagtg aataccagcg ctaccacctg aacgactggt ggccgccggt
     1921 ggcgaacgcc atgatgatgt cgcccaacac cttccagaat ctccacaacg gccagcgcga
     1981 ccgcatggat gtatttgccg agtgggaagc cgcctggtcg ccccgctggc agaccctggc
     2041 cggcctgcgc agcagcaccg tgcgcatgga cgccgacccg gtgcagggct acaacaccat
     2101 ggcgatgatg tacggcgccg acgccgcccg tttcaatgcg gccagccgcg cccgtaccga
     2161 ccacaacctg gacgccaccc tgctggcccg cttcacgccg gatgcgggcc gcagtttcga
     2221 tgccggctac gcccgcaaga cccgctcgcc cagcctgtat gaacgctaca cctggtccac
     2281 caacggcatg gccatgacca tgaacaactg ggtgaacgac ggcaacggct acgtgggcag
     2341 catcgatctg gcgccggaag tcgcccacac cctgagtgcc agcgccgacc tgcacgatgc
     2401 cgggcaggcc cgctgggggg tgaagctgca atccttctac acttatgtga acaactacat
     2461 caacgctcgc tgcctgacga gttgcccgcc taaccagttc aactacctgc gcctcgtcaa
     2521 taccgacgcg cggctgtacg gcgccgacct gtccggctac cgggtgctgg acggcggcga
     2581 gaccctgggc ccgatcaccg gccgcggcac cctggcctac gtgcatggca aggacaccca
     2641 cagcgacgac gggctctaca accagatgcc cctcaacgcc cgcctgagcc tggagcagaa
     2701 gcacggcccg tggaccaccg cggtggagga agtgctggtg ggcgccaagc gcgccgtgtc
     2761 ccaggtgcgc aatgagatcg ccaccggcgg ctacgctctg ctcaacctgc gcagcagcta
     2821 cgacacgcgc cactaccggg tggatctggg cctcgagaat gcactgaaca ggttctacgc
     2881 ctcgcccctc ggcggcgcct atatcggcca gggcaccacc atgtccctca acggcgcggg
     2941 cgccccctac ggcatcgccg tccccagcat gggccgctcc gtgtatgtgg cactgaccgt
     3001 ccgatattga ggggcagacg gcccgcgccg ccgggccgct gtcggacatc cccatgaaaa
     3061 aggccgaagc gcgacatcca tcgcgcttcg gcctcgtcac ggccggggtt gcgggcgggc
     3121 gatcaggcca cggccagcag ctccacctcg aacaccaggg tggcgttggg gggaatcaca
     3181 ccgccggcgc cgcgggcgcc gtagcccagg ttgggcggga tggtgagctt gcgacggccg
     3241 ccgaccttca tgccttgcac gccttcgtcc cagccggcga tcacgtggcg ggcgccgagg
     3301 gggaagacga aggggtcgtt gcggtccttg ctggaatcga acttgcggcc gtcggtgagc
     3361 cagcccgtgt aatgaacctg cacgcgctgg ccggcgctgg cttcggggcc ggtgccgact
     3421 tccagatcct cgatgacgag accgctcgcc gtggtgactt cagacataca gtgctcctat
     3481 ggaaagaccg cccggattct agatcaagtc gaccgcaccc gctgcgggaa cgcgcgcaga
     3541 aacttctgca ccttgggggc gacgatgacc tggcaatagg gctggtggcc gttgcgggaa
     3601 aaataatcgt ggtgataggg ctccgccggc cagaagaccg gcgcgggcac gacttcggtg
     3661 acgatggggg cgtgatacgc gcccgccagg gcggcgatcc gggcctgggc cgccgcggcc
     3721 tgggccgggg tggtggcaaa gatggcggag cgatactggg tgcccacgtc gttaccctgg
     3781 cggttgaggg tggtggggtc gtgaatggcg aagaagatct caagcaggtc gtcgaaggtg
     3841 atgcgcgcgg ggtcgaattc gatccggatg gcttcggcgt ggccggtggt gccgtcgcag
     3901 acctggtgat aggtgggcgc atccacatgg ccgccgatgt agccggaggt gacggtgtgc
     3961 accccttcgg cgagcaggaa gaccgcttcc aggcaccaga agcagccgcc ggccaggatg
     4021 gcggtttcgg aggtcgtgtc ggtcatggtc ggtctccagg agcgtactgc tcattttggg
     4081 cgtgacgggt agacgcgaag ataaaggaga tcgctgacaa aggcgagcca gggcagcgac
     4141 acgcccagca ggggcggcac aaagacggtg gcgatgatcg ccacggccag cacgccggcc
     4201 cacgtcgcca tggccggccc gttggtccag atgccgcgca cgctggccac caccgcgccc
     4261 accagcgtgg cgcgccggtc gaggagcagg ggcaccgcaa aggcggtgat gtggaagacg
     4321 atgagcgcga agacgccgcc catcaggctg gtccacgcgt ggaagcgcca cacctggtcg
     4381 gcccccggcc gcgtgtcgcc gatcatgaag ctatagacgg tggcggcgtc gctcatccag
     4441 atcaggaaca gcagggcgca caccgccgca agggcccaca caccccgcgg cgccgccgcg
     4501 aagccgccga gcagatccgc cagccgcggc gggcgggtcg cggccacccg gcgcacggcg
     4561 aagaagcccg ccagcagcgc cggccccacc agcaggaagc caccggccag gggcaggcga
     4621 agggcgtgca gatcgtaggc ggccagtacc ccgaagagcc ccagcccgag ggcggcgaac
     4681 acactggcga agagcgccgc cggccccgga tggcggcgaa agtcccgccc gccttcggcc
     4741 agggccgccc agacggcgga gacggggatg cggcagggct ggagcggttc ggtcatgggc
     4801 gggcctccgg ggtgcaggcc ccgcatctcg cccgagacgg gccgggccgt ccatgacctg
     4861 catcaagcgc ccgccgccgc cttggcgcgg ccccgcaggc ttcagcggaa ggagagggtg
     4921 aacaccgcgt cgagggcgtt ctcgctgccg gcgcggccca ccacggcgat gcgccgggtg
     4981 agctggtagc tcagcttgac gatgctggag gcgccggtga cgctctgctc gaaggacagc
     5041 agggcgcggg ccgacaggcg cttgccgatg ctcaccacct gaccggcggt ggattcgctg
     5101 ccggtggtgg tgcccgccga cgaggtggtg ccggtgacgc tgcccgtggc aacgctgctg
     5161 gtctggatgc gctggcggct gtccacgctg ctgccaaagg agaactcgtc gaagccgagg
     5221 gcacggacca gaccggagga catgctgtcg gtgcccagca gggcctgggc ggcgggcagc
     5281 agcagggcca gctcgccgcc ggagccctga tcggggggac gcccgaggac gatccaggcg
     5341 agcttttccg ggtcgggcac attgggttcg gagaccaggc gcaccagcgg gcgccgcgcc
     5401 gtgccggtga tggcgactcc ggcttccacc gagaggcctt tgcgcagggc caccacgttg
     5461 aggctggggt tgctcaccgc accggtgaag gtgacgatgc cccgctcgat gtccagcttc
     5521 tggccgtagc cttcgtacac gccgccctgg gtgttgatga cgccggtcat ggcgaggggt
     5581 tggccgtcgc cggcgcgcag gcgcagctcg cccgccagac gggtgtcgag gccgagggcg
     5641 cgcacgtaga gcgcgtcccc caggccgatg gccaggtcca ccgcggtgct gaagggcttg
     5701 accgccggcg ggcgggcctg ttcggggcgc accacctcca cgtcgtcccc caggctgggc
     5761 gccggtgtgc gggagagctc cacgtagccg gcgtcggcgg tgagccggcc gcggatgcgc
     5821 gccgaattcc agccgctgtg cacctcgccc gcaccgctga gcaccagcca ttggtcgggg
     5881 cgttgcagca ggggcaggcg ctcggcctgg aagctgaagg cgccggcgcc gctcgccagg
     5941 gcgacttcgc ccttgacctg cagactgccg ggtgtgcggg tgaggcgcgc cacgtcgatg
     6001 cgcccttcgt tgggtttgac ccggttgggc gaggcgaagt cgaagcgctc cacgcgcagg
     6061 cggtcccggt tgaagtcggc caccagggta ccgccggaca ggttgaggcc ctggtcggcc
     6121 atcgcgaagg ccagcgcctc gccgcggatg cgcccttcgg tgacgggctg ggcgggcgtg
     6181 ccggacaggg tgaagtcggc cttcaggcgg ccggcggtct tgaggttggg gtcggccagg
     6241 gggccggtcc atgcgatgct gggcatgtcc agctgggccg agcccagcag gggcgcgtcg
     6301 ggcaccaggc gcacgccgcc gtccgccagg cgctcggcca gggcggtgcc ggcgccggag
     6361 gccttgccca gctgcgcgcc gctcaccgag aagccgaagg ccagacggct ggccaccgcg
     6421 ttgacgacga tctgcacttc atccagcccc aggcgtaccg gcgcgtcgcc cagcaccagc
     6481 aggtcgcccc gctcccggaa gagatgcacg gtgccgtcca cgtggccgcc cacgtggatg
     6541 tcccagtcgc cgcccatgcg cagagtgtcg cccagcgcgg cgcggttcgc gccggcggcg
     6601 tcgaggccga tgccgaccgg cagcccggac agccggccgc gggcgacgat ctcgccatcc
     6661 gcccagcggg tctcgtcaag acgcagggcg gcgccggcct cgctgcgcag ttcggccgcc
     6721 ccgatgcgca cttcggccgg ggccagcacc agcggcaccg gcgctgccag cgtcgtcgca
     6781 tggggcccga ggacgcgcag ccggtcgagg cgtccctgcc agcgcagggc gtcgcccagg
     6841 ccgccggaga gatccagctc gagggcatcc gcaccgggcg tgtgggcttc caggtgggcg
     6901 cggtgggcac tgcgccggcc atcgacgacc agactggcgc ggcgcaggcc atgctccagg
     6961 tccgcgcccg tgcgcacacc ggccaggttg aggagcaggt tcagcggcgc atcggcgccc
     7021 tcctccagcc ggccccgcgc attgacgccc tccacatgca cggtgccata ggcgaggcct
     7081 tcgccgctca gctccagctc gccggccggg cgggcgggcg tgccgcgcag cacggcagcg
     7141 gccgtgacgc gaccgctgag ggtgtgcccg agggcggcga gatcgggggc ctccaggtgc
     7201 aggtcgaggg catcgcccgg ggcaccgtag cggccacggg cggacaggcg gttggcggct
     7261 acggcaagcg tcaggtcggc gtcgctgagg cgccggccgt ccacggcgag ggtgccgtgg
     7321 ccgacgaggg gccggccctc cagcgtgctg ggggccagct ggaagctcag cgttgcggcc
     7381 atctgcgggc tgcgccggcc gcgggcctcc aggtccaggg tgaggtcggc ggcgggcgcc
     7441 tccgtgaaga aggcgtgggg atcgaagtga tcgaggcggc ccttgagctc gaagcggccg
     7501 tcgccggcca ggtcggccgc gccgcggacg ctgaggcggg ccttgccggc gcgggcgagg
     7561 agctcctgca ccgtgaccgc gccgtcggca tggcgggcgg cgatgcgcag cgtgcgcttc
     7621 tcgccgttgc caccgatcca gatgcggggc cgggcctgta ccgggcgggg cttgcacgcc
     7681 gcgttttcca accggtagta gttgccttcg aagttcgagc gctcctgcgt ggtgagaagg
     7741 cgaacgacct ggacgccctc ttcgagcatg tccatgcgct ccttgatggg gcggaagggg
     7801 atgc
//
LOCUS       DAOQPA010000141         7753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5215_length_7753_cov_18.467264, whole genome shotgun sequence.
ACCESSION   DAOQPA010000141 DAOQPA010000000
VERSION     DAOQPA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_7753_cov_18.467264"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="metG"
                     /locus_tag="PKX01_14980"
     CDS             <1..1125
                     /gene="metG"
                     /locus_tag="PKX01_14980"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HMZ77486.1"
                     /translation="GAKMSKSRGTFITARSYTEQKLNPEWLRYYFAGKSNGTMEDVDL
                     NLDDMIAKVNSDLVGKFVNIASRCAGFIAKRFDGKLAAHDSALDLAWSADEVAAAYEA
                     RDYSRALRRIMEFADQANAYVNDHKPWELAKQDGQAARLHVVCSTALNLFKALSGLLK
                     PVLPALASQVETFLRIEPLQWADVLTPLPAGHAILSYSHLMTRIERKQIDALLDANRE
                     SLAPAAATPAKADKTTQPAHSEQRHAQHQAHAAKVEAAHASAFEPFIGIDDFAKVDLR
                     IARIVSAEHVDGAAKLLRLQLDIGEAQPRQVFAGIKSAYDPATLVGRLTVMVANLAPR
                     KMKFGMSEGMVLAASDESGTHAGLFILSPDAGAVPGMRVK"
     gene            1173..1454
                     /locus_tag="PKX01_14985"
     CDS             1173..1454
                     /locus_tag="PKX01_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77487.1"
                     /translation="MTERSVPTLRLPPAFFGWALVDLIGVAVMAVGAAWLIEGRASLL
                     PGFPANTLQAWICVVGGLGTLFVAAVKMLVEVMHANKRGGAANDDAFGR"
     gene            1586..3274
                     /locus_tag="PKX01_14990"
     CDS             1586..3274
                     /locus_tag="PKX01_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ77488.1"
                     /translation="MKKISFHPKLLDTLPGYDAGQFGRDLSAGLTVGVLALPLAMAFA
                     IASGMSPTAGIWTAIVAGFLISALGGSRVQIGGPTGAFIPILYGIVVEYGVQNLLVAT
                     MLAGAMLVLMGVFRVGNVIRFIPKTVVIGFTNGIAVVIFLAQIKDFLGLRIEHLPGEF
                     FGKVHALFTHLDTLDVPTLALSVASLAVLLSWNGVARRYPALGRVPGPLGVLVVATVA
                     NAILHLPVETIGSRFGGIPQEIPPLALPDLTLATLGKLISPALTIALLGAIESLLSAR
                     VADATIEDRHDPNQELIGQGIANLIAPLFGGFAATGAIARTATNVRTGGRTPVAGMLH
                     AGVLLAIVLALAPFARHIPLATLSAIVVVVAVNMGEWHEFRGLGRFSVNYRTTLLSTF
                     LVTVVFDLTLAVELGMVLASLFFIYRMSDLTRVEREPLPELTLLPHFLYPDGSPRVVA
                     YALYGSLFFGAIHKLETLLDPAERHPEVVILDMLHLVNLDTTGLEGLESLCKNLHRKG
                     CALLLCTLNPQPESLIRRSGFAEEVGVNNLCADMYAALARAKVLLPSFMDEDNL"
     gene            3258..>3512
                     /locus_tag="PKX01_14995"
     CDS             3258..>3512
                     /locus_tag="PKX01_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HMZ77489.1"
                     /translation="MKTTSDRSGRLLRIHGLVQGVGYRNALLAKARRLGLDGWVRNRS
                     DGTVEALAEGPTTAVNTLIEWARRGPPAARVSGVDAIPADS"
     assembly_gap    3513..3612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3613..6067
                     /locus_tag="PKX01_15000"
     CDS             <3613..6067
                     /locus_tag="PKX01_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77490.1"
                     /translation="REFPLFLFEHLPTLIWRSGPDSQCSYFNQTWLDFTGRSLAEEIG
                     DGWTLGVHPEDFDRCVGTYLQHFAARTPFEMEYRLRRHDGEYRWILDTGRPIVTPSGE
                     FAGYIGSCLDITDRRNTEATLKERENRMTTLLASMGEGVIMRDAGGDILMHNAAAERI
                     LGLTASDLARWNVFSPFLAYVNEEGVPVEPAQLPAMQAIASGEPRSGVIGIDRPDGQR
                     RWLLTNCVPLFNNGEKQASAVVTTIADITERRLHEEQLRLAWTVFRNSVEAILVTDAS
                     HRIISVNKAFCEVTGYSEAEAIGATPRLLKSGLHDRPFYDAIWAAIDTAGFWQGEIYD
                     RRKDGSLYPAAMSISAVHDRSGRLTHYVSVFSDITERKASEARIAYLAQHDALTGLPN
                     RALLHDRLEQALAAAARHDRRLAVLFLDLDRFKTINDSLGHLIGDRLLKGVAERISAC
                     IRETDTVSRQGGDEFLIVLTDFNIPDDAARVAEKILDRITAPFDLDGHQLGTSFSIGI
                     ALYPDDGTTPETLMKNADTAMYHAKESGRNTYRFFDEAMNANALERLHLENALRRAQE
                     HAEFELHYQPQVDLVTGRIIGLEALLRWFSPTLGNVSPVRFVPLAEECGLIVPIGEWV
                     LHEACRQARAWQDLGLPAVPVAVNLSALQFRRNDIVATVSQALAESRLAGAWLELELT
                     ESLLMQSGPDIVQILKQLKALGVRMSIDDFGTGYSSLAYLKRFPVDQLKIDRSFVRDL
                     TEDPDDAAIVRAVVQLGRSLRLDVIAEGTETPEQLDFLRNEGCAAAQGYVFSPPLPPA
                     AITELLRIGVLPAAPLMPM"
     gene            complement(6054..6533)
                     /locus_tag="PKX01_15005"
     CDS             complement(6054..6533)
                     /locus_tag="PKX01_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HMZ77491.1"
                     /translation="MSASFLDADLLDGLVTQARSVPRRRAHHNLHTDLAAPAQRLLVA
                     MEPDSFVRPHLHLDPHKAETLVILRGRLGVLLFAPDGSVIGTRLLVPGGACLGYDVAP
                     GCLHAVIALEPGSVFLEAKAGPYIPLAAAEIPAWSPAEDSPEAAGCLRRWAALFTSA"
     gene            complement(6530..>7753)
                     /gene="dnaE"
                     /locus_tag="PKX01_15010"
     CDS             complement(6530..>7753)
                     /gene="dnaE"
                     /locus_tag="PKX01_15010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ77492.1"
                     /translation="GFNKSHTAAYAVVTYHTAWLKAYHCAAFMAATMSSDLDNTDTVK
                     IFYEDTLANGITVLPPDVNCSDYRFVPTDARTLRYGLGAVKGVGEPAVRAILAARSEG
                     GPFKDLFDFCQRVDKRLANRRVIEALIRAGAFDAIAPDQPAERARLLASVGIAMEAAE
                     QIAANALQGGLFDFADEPAAPMVDYVSVRPWGEREKLKEEKLAIGFFLSGHPFNAFRD
                     EVRRFVRRPLSQVEPGKELIMLAGVVMEVRAKMTARGKIAFVLLDDGTMPREVSVFSE
                     VFDANRQRIVVDEVLVVEAKVSNDDFSGGLRIVADRLMTLGEARGRFARALQIRLNGE
                     VAEAGGAAAAAERLQTTLAPFREGECPIRVRYRNAEAEADFPFGSGWRVRPEDGLLDS
                     LREWLPPEAVEVVYG"
BASE COUNT         1220 a         2866 c         2352 g         1215 t
ORIGIN      
        1 ggcgccaaga tgagcaagag ccgcggcacc ttcatcaccg cccgttccta caccgaacag
       61 aagctcaacc ccgagtggct gcgctactac tttgccggca agtccaacgg caccatggaa
      121 gacgtggacc tgaacctgga cgacatgatc gccaaggtca attccgatct ggtaggcaag
      181 ttcgtcaata tcgccagccg ctgcgcgggc ttcatcgcca agcgcttcga cggcaagctc
      241 gccgcccacg acagcgccct cgacctggcc tggagcgccg acgaggtggc ggctgcctac
      301 gaggcccgtg actacagccg cgccctgcgc cgcatcatgg aattcgccga ccaggccaat
      361 gcctacgtaa atgaccacaa gccctgggag ctcgccaagc aggacggcca ggcggcgcgc
      421 ctgcacgtcg tgtgctccac cgccctcaac ctcttcaagg ccctcagcgg actgctcaag
      481 ccggtgctcc cggccctcgc cagccaggtg gagaccttcc tgcggatcga gcctctccag
      541 tgggccgatg tcctcacacc acttccggcc ggccatgcca tcctgagcta cagccacctc
      601 atgacccgta tcgagcgcaa gcagatcgat gcgctcctcg acgccaaccg ggaatccctc
      661 gcccccgcgg cggccacgcc ggccaaggca gacaagacca cccagcccgc ccactcggag
      721 cagcgccacg cccagcatca ggcccacgcg gccaaggtcg aagcggccca tgcctcggcc
      781 ttcgagccct tcattggcat cgacgacttc gccaaggtgg acctgcgcat cgcccgcatc
      841 gtctctgccg agcacgtgga cggtgccgcc aagctgctgc gcctccagct cgacatcggc
      901 gaggcgcagc cacgtcaggt cttcgccggc atcaagtccg cctacgaccc ggccaccctg
      961 gtcggccggc tcaccgtgat ggtggccaac ctcgccccgc gcaagatgaa gttcggaatg
     1021 tcagaaggca tggtgctcgc cgcctccgac gagtcgggca cccatgcggg gctgttcatc
     1081 ctgtcgccgg acgccggcgc cgtgcccggc atgcgcgtca agtaaggccg gcgtaagcct
     1141 gccgtcagcc tcttctcgca cagtgcccgc ccatgactga acgctccgtc cccaccctgc
     1201 gcctgccgcc cgccttcttt ggctgggcgc tggtggatct catcggcgtc gccgtgatgg
     1261 cagtgggcgc cgcctggctc atcgagggcc gcgcatccct actgcctggc ttcccggcca
     1321 acaccctgca ggcctggatc tgcgtggtcg gcggtctggg caccctgttc gtggccgccg
     1381 tcaagatgct ggtggaagtg atgcacgcca acaagcgcgg tggcgccgcc aacgacgacg
     1441 ccttcggccg ctagagcttc cctcaccatg cccctcaccg cctgcaaacg atggttcgtc
     1501 cagcatcgta ccgggccggg gccgaagcct ccgcacgtgg cgccacccgg cgccttcgtt
     1561 ccaatcgacc gattgcggag gttccatgaa gaagatctcg ttccacccca agctccttga
     1621 caccctgccc ggctacgatg ccggccagtt cggccgcgac ctgtccgccg gcctcaccgt
     1681 gggcgtcctc gccctgcccc tggccatggc cttcgccatc gcctcgggca tgtcccccac
     1741 cgccggcatc tggacggcca tcgtcgccgg cttcctgatc tccgcgctgg gcggctcgcg
     1801 ggtgcagatc ggtggtccca ccggggcctt catccccatc ctgtacggca tcgtcgtcga
     1861 gtacggcgtt cagaacctgc tggtggccac catgctggcc ggcgccatgc tggtattgat
     1921 gggcgtgttc cgtgttggca acgtgatccg tttcatcccc aagaccgtgg tgatcggctt
     1981 caccaacggc atcgcggtgg tgatcttcct cgcccagatc aaggacttcc tcggcctgcg
     2041 catcgagcac ctgccgggcg agttcttcgg caaggtgcat gccctgttca cgcacctgga
     2101 caccctcgac gtgcctaccc tggccctgtc cgtcgcctcg ctggcggtac tgctgtcgtg
     2161 gaacggcgtg gcccggcgct accctgcact ggggcgcgtc cccggtcccc tcggggtcct
     2221 cgtggtggcg accgtggcca atgccatcct gcacctcccg gtggaaacca tcggcagccg
     2281 ctttggcggc attccccagg aaattccgcc cctcgcgctg cccgacctga cgctcgccac
     2341 cctgggcaag ctgatttccc cggccctcac catcgccctg ctgggcgcca tcgagtcgct
     2401 cctgtcggcg cgggtcgccg acgccaccat cgaggatcgc cacgacccca accaggaact
     2461 catcggccag ggcatcgcca acctgattgc gcccctcttc ggcggattcg ccgccaccgg
     2521 cgccatcgcc cgcacggcca ccaacgtgcg taccggcggc cgcacgcccg tggccggcat
     2581 gctccacgcc ggcgtgctgc tcgccatcgt gctggccctg gcgccctttg cccgccacat
     2641 cccgctggcc accctgtcgg ccattgtcgt ggtggtggcg gtcaacatgg gcgaatggca
     2701 cgagttccgc ggcctcggcc gcttctcggt gaattaccgc accaccctgc tgtccacctt
     2761 cctcgtcacc gtggtcttcg acctgaccct cgccgtggaa ctgggcatgg tgctggcgag
     2821 cctgttcttc atctaccgca tgtcggacct gacccgggtc gagcgggagc ccctgccgga
     2881 gctcaccttg ctgccccact tcctgtaccc ggacggctcg ccgcgggtcg tggcctacgc
     2941 cctctacggc tccctgttct tcggcgccat ccacaagctg gagaccctcc tcgaccccgc
     3001 cgagcgccac ccggaggtgg tgatcctcga catgctgcac ctggtcaatc tggacaccac
     3061 cggcctcgaa ggtctcgaaa gcctgtgcaa gaatctccac cgcaagggct gcgcgctcct
     3121 tctgtgcacc ctcaatccgc agcccgaatc cctcatccgc cgctcgggct tcgccgagga
     3181 ggtgggcgtc aacaatctct gcgccgacat gtacgcggcc ctcgcccgcg ccaaggtgct
     3241 gctgccgagc ttcatggatg aagacaacct ctgaccgtag cggccgcctg ctgcgcatcc
     3301 acggcctagt gcagggcgtg ggctatcgca acgccctgct cgccaaggcc cgccgcctcg
     3361 gcctcgacgg ctgggtgcgc aaccgcagcg acggcaccgt cgaagccctc gccgagggtc
     3421 cgaccaccgc cgtgaacacc ctcatcgagt gggcccggcg tggcccgccg gccgcgcggg
     3481 tgagcggcgt ggacgcgata ccggccgaca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnacgggaat tccccctgtt cctcttcgag catctgccca ccctgatctg
     3661 gcggtccggg ccagactccc aatgctcgta tttcaaccag acgtggctgg atttcaccgg
     3721 tcgcagtctg gccgaagaga tcggtgacgg ctggacgctg ggcgtacacc cggaagactt
     3781 cgaccgctgc gtcggcactt acctgcagca tttcgcggcg cggacgccgt tcgagatgga
     3841 ataccggctg cgtcgccatg acggggaata ccgctggatc ctcgacaccg gccggcccat
     3901 cgtcaccccc tccggggaat tcgccggcta catcggctcc tgcctcgaca tcaccgaccg
     3961 ccgcaacacc gaggccaccc tcaaggagcg ggaaaaccgc atgacaaccc tgctcgcctc
     4021 catgggcgaa ggcgtcatca tgcgcgacgc aggcggcgac atcctcatgc acaacgccgc
     4081 ggccgagcgc atcctcggcc tcacggcgtc cgacctggcc cgctggaacg tattctcgcc
     4141 tttccttgcc tacgtgaacg aggagggcgt gccggtcgag cctgcccagc tgcctgccat
     4201 gcaggccatc gccagcggcg agccccgctc cggcgtgatc ggcatcgacc gccccgacgg
     4261 gcagcggcgc tggctgctca ccaattgcgt gcccctcttc aacaacggcg aaaagcaggc
     4321 cagcgcggtg gtcaccacca tcgccgacat caccgaacgg cgcctccacg aggaacagct
     4381 ccgcctggcg tggacggtgt tccgcaacag cgtggaagcg attctcgtga ccgacgccag
     4441 tcaccgcatc atctccgtga acaaggcctt ctgcgaggtg accggctatt ccgaggccga
     4501 ggccatcggc gcgacgccgc gcctcctcaa gtccggcctg cacgaccgcc cgttctacga
     4561 cgcgatctgg gcggccatcg acacggccgg cttctggcag ggcgagatct acgaccggcg
     4621 caaggacggc agcctctacc cggccgccat gtccatcagc gccgtgcatg accgcagcgg
     4681 ccgcctgacc cattacgtgt cggtgttctc cgacatcacc gaacgcaagg cctcggaggc
     4741 gcgcatcgcc tacctcgccc agcacgacgc cctcacgggc ctgcccaacc gggccctgct
     4801 ccacgaccgt ctggaacagg ccctcgcggc ggccgcccgc cacgaccgtc gtctggccgt
     4861 gctgttcctc gacctggacc gcttcaagac catcaacgat tccctcggcc atctcatcgg
     4921 tgaccgtctg ctcaagggtg tggccgagcg gatcagcgcc tgcatccgcg agaccgatac
     4981 ggtgagccgc cagggcggcg acgagttcct gatcgtcctc accgatttca acatccccga
     5041 cgacgccgcc cgcgtggcgg aaaagatcct cgaccgcatc acggcgccct tcgatctgga
     5101 cggccaccag ctcggcacct ccttctccat cggcatcgcc ctctatccgg acgacggcac
     5161 caccccggaa accctgatga agaacgccga cacggccatg taccacgcca aggagagcgg
     5221 ccggaacacc taccgcttct tcgacgaggc catgaacgcc aacgcgctgg agcgcctaca
     5281 cctcgagaac gccctgcgcc gggcccagga gcacgcggaa ttcgagctgc actaccagcc
     5341 ccaggtggac ctggtgaccg gccgcatcat cggcctggaa gccctgctgc gctggttcag
     5401 cccgaccctc ggcaacgtgt cgcccgtgcg cttcgtgccg ctggccgagg aatgcggcct
     5461 gatcgtgccc atcggcgaat gggtcctcca cgaagcctgc cgccaggccc gcgcctggca
     5521 ggacctggga ctgcccgccg tgccggtggc cgtcaacctg tcggccctgc agttccgtcg
     5581 caacgacatc gtcgccacgg tctcccaggc gctggccgaa agccgtctgg ccggcgcctg
     5641 gctcgaactg gagctgaccg agtccctgct gatgcagtcc ggcccggaca tcgtgcagat
     5701 cctcaagcag ctcaaggcgc tgggcgtgcg catgtccatc gacgacttcg gcacgggcta
     5761 ctccagcctg gcctatctca agcgctttcc ggtggatcag ctcaagatcg accgcagctt
     5821 cgtgcgcgac ctgacggaag atccggacga cgcggccatt gtgcgggccg tggtgcagct
     5881 cggccgcagc ctgcgtctgg acgtgatcgc ggagggtacc gaaacccccg agcagctgga
     5941 cttcctgcgc aacgaaggct gcgccgcggc acagggctat gtgttcagcc cgcccctgcc
     6001 gccagccgcc atcacggagc tgctgcgcat cggcgtactg cccgccgccc ccctcatgcc
     6061 gatgtgaaca aggccgccca gcgacgcaga cacccggcgg cctcagggct gtcttctgcc
     6121 ggcgaccagg cgggaatctc ggcggccgcc agcggtatgt aggggcccgc tttcgcttcc
     6181 aggaagacgc tgcccggctc cagcgcgatc acggcgtgaa ggcagcccgg cgccacgtca
     6241 taccccaggc aggcgccccc gggcaccagc aggcgcgtcc cgatcacgga cccgtctggc
     6301 gcgaacagca gcacccccag ccgcccgcgc aggatgacca gcgtttcggc cttgtgcgga
     6361 tcaaggtgca ggtgcggccg tacgaaggag tccggctcca tcgccaccag caggcgctgg
     6421 gcgggggcgg cgaggtccgt gtggaggtta tggtgcgccc gccttcgcgg gacgctgcgg
     6481 gcttgcgtca ccagcccgtc caggagatcc gcatcgagaa agctcgcact catccgtaca
     6541 ccacctccac tgcctcgggc ggcaaccatt cccgcaggct gtccagcagg ccgtcctcgg
     6601 gccgcacgcg ccaacccgat ccgaacggga aatcggcctc cgcctcggcg ttacggtagc
     6661 gcacgcggat cgggcattcg ccctcgcgga agggcgccag cgtggtctgc aggcgctcgg
     6721 ccgcagctgc tgcgccgccg gcctcggcga cctcgccatt gaggcggatc tgcaaggcac
     6781 gggcaaaacg gccgcgggcc tcgccgaggg tcatcaggcg gtcggcgacg atgcgcaggc
     6841 cgccggagaa atcgtcattg ctcaccttgg cctccaccac cagcacttcg tccaccacga
     6901 tgcgctggcg gttggcgtcg aagacttccg agaacaccga cacctcccgc ggcatcgtgc
     6961 cgtcgtccag cagcacgaag gcgatcttgc cgcgggcggt catcttggcg cgtacctcca
     7021 tgaccacgcc ggccagcatg atcagttcct tgcccggctc gacctgggac aggggccgcc
     7081 gcacgaagcg ccgcacctcg tcccggaagg cattgaaggg gtggccggac aggaagaaac
     7141 cgatggcgag cttctcttcc ttgagcttct cccgctcgcc ccagggtcgc accgacacgt
     7201 aatcgaccat cggcgccgcg ggctcgtcgg caaaatcgaa caggccgccc tgcagggcat
     7261 tggcggcgat ctgctcggcg gcctccatgg cgatgcccac cgaggcgagc aggcgggcgc
     7321 gctcggcggg ctggtccggc gcgatggcat cgaaagcgcc agcgcggatc agggcctcga
     7381 tgacgcgccg gttggccagg cgcttgtcca cccgctgaca gaagtcgaac agatccttga
     7441 acggtccgcc ctcgctgcgc gccgcgagga tggcccgcac cgccggctcg cccacgccct
     7501 tgaccgcgcc cagaccgtag cggagagtcc tggcgtcggt gggcacgaag cggtagtccg
     7561 aacaattgac gtccggcggc agcacggtga tgccgttggc gagggtgtcc tcgtagaaga
     7621 tcttgaccgt gtccgtgttg tccaggtcgg aggacatggt cgccgccatg aaggccgcgc
     7681 agtggtaggc cttgagccag gcggtgtgat aggtgacgac ggcgtaggcg gcggtgtggg
     7741 acttgttgaa gcc
//
LOCUS       DAOQPA010000142         7690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5280_length_7690_cov_18.977996, whole genome shotgun sequence.
ACCESSION   DAOQPA010000142 DAOQPA010000000
VERSION     DAOQPA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_7690_cov_18.977996"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..465
                     /locus_tag="PKX01_15015"
     CDS             184..465
                     /locus_tag="PKX01_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ77493.1"
                     /translation="MSVPSDFAAWRDAALADGFDEVLERRWPPDTLLETHTHPFALRA
                     VVVEGELWLTTAGESRHLEPGDCFELMRDEPHAERYGTQGAVYWVARRN"
     gene            complement(514..2139)
                     /locus_tag="PKX01_15020"
     CDS             complement(514..2139)
                     /locus_tag="PKX01_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ77494.1"
                     /translation="MLRISELRLPLDHPPEALRAALVQRLGVDDADLLAFTVFKRSYD
                     ARKAQHLTFIYIVDAELRDEAAVLARFAGDAHIKPAPDMTYRFVGTAPPDIAHRPIVI
                     GFGPCGIFAALILAQMGFKPLVLERGKAVRERTQDTWGLWRRNVLNPESNVQFGEGGA
                     GTFSDGKLYSQIRDPKHLGRKVLTEFIKAGAPPEILYVSKPHIGTFRLVGMVETMRAE
                     IQSLGGEIRFGQRVTDLLIEDGHVRGVRTATGDELRSDHVVLALGHSARDTFEMLHAQ
                     GVFMEAKPFSVGFRIEHPQSLIDRARLGPHAGHPELGAADYKLVHHARNGRTVYSFCM
                     CPGGTVVAATSEPERVVTNGMSQYSRNERNANAGIVVGIDPRDFPGSGPLAGIALQRE
                     LESRAYVLGGRSYEAPGQLVGDFLRGIPSTALGDVEPSYKPGVRLGDLSTALPDYAIA
                     ALREALPAFDRQIKGFNRYDAVLTGVETRTSSPLRITRGEDCQSLNVKGLFPAGEGAG
                     YAGGILSAGVDGIRVAEAVARDMLGEAVNAVED"
     gene            complement(2261..4129)
                     /locus_tag="PKX01_15025"
     CDS             complement(2261..4129)
                     /locus_tag="PKX01_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HMZ77495.1"
                     /translation="MKPNRRTLLKGGAAGLLSALIAPPGLAQTASSVLGASKGRQFSQ
                     LMPNPLDPANLFAPDMFGGTTYTLKIREFSANLGLVNPATGAPLPTTLWGYGTANQAP
                     TYPGRTFNVRQNKAITVRYQNELLNAAGPLPHRLPVDTTIDWANPGNLGGRAPVPLVA
                     HRHGGDQGTLSDGLPDAWSTPDANRDGMPDYRGRIYSAAYTFDNSQEAGHLWYHDHAL
                     GVTRLNVYMGLAGNYFIRDSNEDVLVRYGHLPAYPYEVPVCIQDRSFTSTGALNYPAT
                     DPANPSAPTPTHLPEFFGDIIVANGVPWPRMDVEPRVYRLRLLNGSDSRVYTLSLAAE
                     KFPNTPLTFWQIGTDLGLMNAPVALTALTLAPGERADVLVDFSRIAWWYHGKVIVRNS
                     ANTPFPNGAPPVAGLTDRVMAFSVTKPLGYYGFSFSALSALCLPGNLRPVLGPVPMPS
                     AANAKVRKLMLFEGTDAYGRLQTMLGTVNPAPGNPAGPGFGTFLYTDPVTENIKVGST
                     EIWEIHNTTVDAHPIHLHLANFRVLDRQPFTGTLVPKPMGPGGIASGGYLTQVNVGGT
                     RRPALANEQGRKDTVLAYPGEVTRIIATFNRPGEYVWHCHILSHEDHEMMRRFVVA"
     gene            complement(4383..4976)
                     /locus_tag="PKX01_15030"
     CDS             complement(4383..4976)
                     /locus_tag="PKX01_15030"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HMZ77496.1"
                     /translation="MNKKNTLLAALAVIVGVFILIISLPDRSDPPLPVPLVAAPGEPI
                     PGRVPETDRFRLHGIIGESAGRAGSALIAEGDGPALHVSEGGELRPGVRLERVLPDRV
                     LLRLDGVAAPIELTLAVTVASTPELLAPPPLATSTGSGGDHELPGPPRGSAPPIQPAI
                     LPRDRVAAPIDASGPPPGTGLTGPALSGPSPGGSTEK"
     gene            5206..5868
                     /locus_tag="PKX01_15035"
     CDS             5206..5868
                     /locus_tag="PKX01_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ77497.1"
                     /translation="MIDLFSRCPAQSLRLAARVLALCAVMSSTAVRADDPGHHVHQDH
                     HAHHLMPPPSAAVVRSEQKVVLPPVGQRSAEGVNKSFAEFVGEGRPVVVNFIYTTCTA
                     ICPPMTRVFAEVQERLGAERESVRLISVSIDPEQDTPRRLKAYGAQYGAVAPWVFLTG
                     SEAASTSIQQAFNVFRPDKMGHTPVTFIRRQPGESWIRLDGIASAERILAEVHGVAGG
                     RR"
     gene            6171..7442
                     /locus_tag="PKX01_15040"
     CDS             6171..7442
                     /locus_tag="PKX01_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77498.1"
                     /translation="MALQDYGFRTRPAYDDISLARALREGIGAGGARFDGLMPRYDLS
                     SRDIAALTAYLHSLDATPADGIDPQPGGAIRLATVILPDADPSQSAAMRAVLSTCVAE
                     RSPRPGSGRRPWTLDEWALSGPPETWRGQLEAFRMERPVFALLGGIGRDWAHVHAFCE
                     ANALPCLFPNTDAPGEQPPPHASMYLSPGVGVEAGVIAADLDESGDMPERLVQVFQRR
                     DIGRYGAAALASALPGIPNRAVSSGADPGWIRAGEVLVLWLAPTDLASFFMAVPEPPD
                     GVRVYLSASLAGFDTGVDTSLIPPPWRPAVRVVYPFDLPDLRLGRQVFNLGGWMAGHG
                     LALVRSTARVQGDSFSACEVVARALYTLPEHPTREVLLERVEEAYAGAVATAYPRFTL
                     GPGQRFGSKGAYLLKIMPDGKWQPQGAWRVP"
BASE COUNT         1154 a         2281 c         2888 g         1367 t
ORIGIN      
        1 caccgcttcc gccagacgcc gctggggtgt cattcccgtt ggggcccgat atgcgcgtac
       61 ccgatggacg atccggtcga tgcgcactgt cgtgccaggc atcgcgtccc gctgcctgcg
      121 ggtggtccga agcctgagcg cggtggggat cacggacatg cggggtgtcg cggaggaggg
      181 ggcatgagcg ttccatctga ttttgctgcg tggcgtgacg ctgcactggc cgacggattc
      241 gacgaagtgc ttgagcggcg ctggcctccc gatacgctgc tggagaccca cacccatccg
      301 tttgccctgc gggcggtcgt cgtggaaggc gagctgtggc tgaccacggc gggtgaatcc
      361 cgccacctgg agcccggtga ttgctttgag ttgatgcggg acgagccgca cgcggaacgc
      421 tatggcacgc agggcgcggt ctattgggtg gcgcgccgga actgagatcg ccggccggca
      481 tggggcgacc tggtggtcgg gattgtcctg cgttcagtct tccaccgcat tcaccgcctc
      541 ccccagcatg tcccgcgcca cggcttcggc cacccggatg ccatccacgc ccgccgagag
      601 gatgccaccg gcgtagcctg cgccttcgcc ggctgggaag aggcctttca cgttgaggct
      661 ctgacagtct tcgccgcggg tgatgcgcag gggcgaggag gtgcgcgtct cgacgccggt
      721 gaggaccgcg tcgtatcggt tgaagccctt gatctgccgg tcgaaggcgg ggagggcttc
      781 acggagggcg gcgatggcgt agtcgggcag tgcggtggac aggtcaccca ggcgcacgcc
      841 gggcttgtag gaaggctcca cgtcgcccag ggcggtggac gggatgcccc gcaggaagtc
      901 gcctacgagc tggccggggg cttcgtagct gcgtccgccc agcacgtagg cgcggctttc
      961 gagctcgcgc tgcagggcga tgcccgcgag ggggccgctg ccggggaaat cgcgcgggtc
     1021 gatgcccacc acgatgccgg cgttggcatt gcgctcgttg cgggaatact ggctcatgcc
     1081 gttggtgacg acccgttccg gctcggaggt ggcggccacc acggtgccgc cagggcacat
     1141 gcagaagctg tacacggtgc ggccgttgcg ggcgtggtgc accagcttgt agtcggcggc
     1201 gcccagttcc gggtggccgg cgtgggggcc gaggcgggcg cggtcgatga gggactgggg
     1261 atgctcgatg cggaagccta ccgagaaggg tttggcttcc atgaacacgc cctgggcgtg
     1321 gagcatctcg aaggtgtcgc gggcgctgtg gccgagggcg aggacgacgt ggtcgctgcg
     1381 cagttcgtcg cccgtggcgg tgcgcacgcc gcgcacgtgg ccgtcttcga tgaggaggtc
     1441 ggtgacgcgc tggccgaagc ggatctcgcc gcccagggac tggatctcgg cgcgcatggt
     1501 ctcgaccatg ccgaccagcc ggaaggtgcc gatgtggggc ttggagacgt agaggatctc
     1561 gggcggggcg ccggccttga tgaattccgt gaggaccttg cggccgagat gcttgggatc
     1621 gcggatctgg ctgtagagct tgccatcgga gaaggttccg gcgccgcctt cgccgaactg
     1681 gacgttggat tccgggttga gcacgttgcg ccgccacagg ccccaggtgt cctgggtgcg
     1741 ttcgcgtacg gccttgccgc gttccagcac gaggggcttg aagcccatct gggcgaggat
     1801 cagcgcggcg aagatgccgc aggggccgaa gccgatcacg atggggcgat gggcgatgtc
     1861 cggcggcgcc gtgccgacga agcggtaggt catgtccggt gcgggcttga tgtgggcgtc
     1921 accggcgaag cgggcgagca cggcggcctc gtcgcgcagt tcggcgtcga cgatgtagat
     1981 gaaggtgagg tgctgggcct tgcgggcgtc gtagctgcgc ttgaagacgg tgaaggcgag
     2041 caggtccgcg tcgtccacgc cgagacgctg cacgagggcg gcgcgcaggg cctcgggcgg
     2101 atggtcgagg gggagccgga gttcggagat gcgcaacatc ggaacagggc gggcggacgg
     2161 cccgcgggag gcgatcggga cgcgatttta gcccggatcg ggccggcccg gttccgtgcg
     2221 ggcctggcgc agcggtgcgc gcgccaagcc cggagggccg tcaggcaacg acaaagcggc
     2281 gcatcatctc gtggtcttcg tgggacagga tgtggcagtg ccacacgtat tcacccggcc
     2341 ggttgaaggt ggcgatgatg cgggtcacct cgcccggata ggccagcacc gtgtccttgc
     2401 gcccctgctc gttggcgagg gccgggcgcc gagtgccgcc gacgttgacc tgggtgaggt
     2461 agccgccgga ggcgattccg cccggaccca tgggcttggg gacgagggtg ccggtgaagg
     2521 gctgccgatc gaggacgcgg aagttggcga gatgcagatg gatggggtgg gcgtctacgg
     2581 tggtgttgtg gatctcccag atctcggtgc tgcccacctt gatgttctcg gtgaccgggt
     2641 cggtgtacag gaaggtgccg aatccggggc cggccggatt gccgggcgcc ggattgacgg
     2701 tgccgagcat ggtctggagg cggccgtagg cgtcggtgcc ttcgaacagc atgagcttgc
     2761 gtaccttggc gttggccgct gatggcatcg gcacggggcc cagtaccggc cgcaggttgc
     2821 caggcagaca caaggccgac aaggccgaga agctgaagcc atagtagccc aggggcttgg
     2881 tgacgctgaa cgccatgacc cggtcggtga gtccggccac cggcggtgcg ccgttgggga
     2941 agggcgtgtt ggcgctgttc cgcacaatca ccttgccgtg ataccaccag gcgattcggg
     3001 agaaatccac cagcacgtcg gcgcgctcac ccggcgcgag ggtcagggcg gtcagggcga
     3061 ccggggcgtt catgaggccc aggtcggtgc cgatctgcca gaaggtgagc ggcgtgttgg
     3121 ggaatttttc ggcggccagg ctgagggtgt agacgcggga gtcggagccg ttgagcaggc
     3181 gcaggcggta gacgcggggt tccacgtcca tgcgcggcca gggcacgccg ttggccacga
     3241 tgatgtcgcc gaagaattca ggcagatgcg tcggtgtggg tgcggacggg ttggccggat
     3301 cggtggccgg gtagttcagg gcgccggtgg acgtgaacga acggtcctgg atgcagaccg
     3361 gcacttcgta aggataggcc ggcaggtggc cgtagcggac caggacgtcc tcattgctgt
     3421 cgcggatgaa gtagtttccg gcaagaccca tgtacacatt gaggcgggtc acgcccagcg
     3481 catggtcgtg gtaccagagg tggccggcct cctgggaatt gtcaaaggta taggctgcgc
     3541 tgtagatgcg gccccggtag tcgggcatgc cgtcccggtt ggcgtcgggt gtggaccagg
     3601 cgtcgggcag gccgtcgctg agggtgccct ggtcgccacc gtggcggtgg gcgaccagcg
     3661 gtaccggggc gcggccgccc aggttgcccg gattggccca gtcgatggtg gtgtcgaccg
     3721 gcagacggtg gggcagtggt ccggcggcgt tgagcagttc gttctggtag cggacggtga
     3781 tggccttgtt ctggcgcacg ttgaaggtcc gacccggata ggtcggcgcc tggtttgccg
     3841 tgccatagcc ccacagcgtc gtgggcaggg gggcgcccgt ggcaggattg accagaccca
     3901 gattggcgct gaattcccgg atcttgaggg tgtaggtggt gccgccgaac atgtcgggcg
     3961 cgaacaggtt ggcgggatcc agggggttgg gcatcaactg gctgaattgc cggcctttgc
     4021 tggctccgag cacggaggac gcggtctggg cgaggccggg cggtgcgatc agcgccgaga
     4081 gcaggccggc cgcgccacct ttcaggaggg ttcgtctgtt cggtttcatc gtgaaatcct
     4141 ttgcaaggag gaggggtgtg gcgtagtggc gcccgtcgca cgtccaccgt cggcgagacg
     4201 ggcacgtgcg tgatgtgggg cacgtgcgtg gtgtgcgtcg aatggcgagg acgctgcgct
     4261 tgggcggaaa tgttcggtta caaatggtcc gactgttttt gatgaatttg ggtgaataaa
     4321 ggtgaaattc ccagcggtgt gagaatttct tgcgaacttt cgcgatggtt tggcggaatc
     4381 gactattttt ccgtcgagcc acccgggcta gggccactga gggccgggcc cgtgaggccg
     4441 gtgccaggcg gcggaccgga ggcatcgatg ggggcggcca cacggtcgcg cgggagtatg
     4501 gcaggctgaa tgggcggtgc gctgccacgc ggtggaccag gcaattcgtg atcgccgccc
     4561 gagccggtcg acgtggcgag cggcggaggc gcgaggagtt ccggcgtgct ggctacggtg
     4621 accgccaggg tgagctcgat gggggcggcg accccgtcga ggcggagcag caccctgtcg
     4681 ggtaataccc gctcgaggcg cacacccggt cggagttcgc ccccttcgga cacatgaagg
     4741 gccgggccgt cgccttcggc aatcagggcc gaacccgcac gccccgcgct ttctccgatg
     4801 atgccgtgaa gtcggaagcg atccgtttcg ggtacccgtc cggggatggg ttcgccgggg
     4861 gcggccacga gcggaacagg cagaggtggg tccgaacggt ctggaaggct gatgatcagg
     4921 atgaatacgc cgacgatgac ggccagggct gcaagtaggg tgttcttttt attcatgggc
     4981 gggtggcgtt ggcagattca aaggagatgc cctcaccgta ccaccggcag aatttccaaa
     5041 atcattaaat gttcgtgcgt gtaagcaaat ttaacgggat ttgccgcggg tcgaccgaac
     5101 aatgtggcgc ctgtcacggg gcgtgcaggg actggctcgg gacaatccgg ccagcgggcg
     5161 gggaatgtcc ccgccgcacg cctccttccc atggataacc ccatcatgat cgacctgttc
     5221 tcccgatgtc ccgcgcagtc cctgcgcctg gcggcacgtg ttctggcgct gtgcgcagtg
     5281 atgtcctcca ccgccgtccg ggcggatgat ccggggcacc acgtccacca agaccaccac
     5341 gcccatcacc ttatgccacc gccctcggcc gccgtggttc ggtccgagca gaaggtggtg
     5401 ttgccgccgg tcggccagcg cagcgcggag ggcgtgaaca agtcctttgc ggagtttgtg
     5461 ggtgagggcc gtccagtggt cgtgaacttc atctatacga cctgtaccgc gatctgtccg
     5521 cccatgaccc gggtgttcgc cgaagtgcag gagcggctcg gggcggaacg ggaatccgtg
     5581 cgcctgattt ccgtgtccat cgatcccgag caggacacgc cgcggcgcct gaaggcgtac
     5641 ggcgcgcagt acggcgccgt tgcgccatgg gtctttctga ccggttcaga ggctgcgagc
     5701 acgtcgattc agcaggcgtt caatgtgttc cgtccggaca agatggggca cacgcccgtc
     5761 accttcatcc ggcgccagcc gggtgagtcg tggatccgac tcgacggaat cgcatccgca
     5821 gaacggattc ttgccgaggt tcacggcgtg gcgggcggac ggcgttgatg cggtggtggg
     5881 gaccgctgct gttcgcgggc atgatcggag ttgcggcagc ggacgacggg ttgttcgccg
     5941 agggacgtcg cctctatatg gaaggacgtc gcgccgatgg ttcggccctg ctggcccggc
     6001 gcgatggcga cgggcgtctg caggggcgcg atatggcctg tgtccagtgc catcgacgca
     6061 gcggtgccgg cacggtggaa ggtgttgtca tcgtgccgtc gatccagggg cgtgtgctct
     6121 tcaacccggg ttcgccaccc cccgggcacc ttccccgccg cgcagcgggc atggccctgc
     6181 aggactacgg attccggact cggcccgcct acgacgacat cagcctcgca cgggccctgc
     6241 gcgaagggat cggtgcgggt ggtgcccggt tcgacgggct gatgccgcgg tacgacctga
     6301 gttctcgcga catcgccgcg ctgacggcct acctgcacag tctggatgcc acaccggcgg
     6361 acggcatcga tccccaaccc ggcggggcga ttcgcctcgc aacggtgatc ctgcccgacg
     6421 ccgatccgtc gcagtcggcg gccatgcgcg ccgtgctgtc tacgtgcgtt gccgaacgca
     6481 gcccgcgccc ggggagtggt cggcggccct ggacgctgga tgaatgggcg ctgagcgggc
     6541 cgcccgagac ttggcgcggt caactggagg cgtttcgaat ggaacggcca gtcttcgcgc
     6601 tgctgggcgg catcgggcgt gactgggccc acgtgcatgc attctgtgaa gcgaatgccc
     6661 tgccgtgcct gtttcccaat acggacgcgc ccggtgagca gccgccaccg cacgcctcga
     6721 tgtacctgtc tccgggcgtg ggcgtggagg ccggggtgat cgccgcagat ctggatgaat
     6781 cgggcgacat gccggagcgc ctcgtacagg tgttccagcg tcgggatatc ggccggtacg
     6841 gcgcggcggc actggccagc gccttgcccg gcattccgaa ccgggcggtg tcgtccggcg
     6901 ctgatccggg ttggatccgc gccggtgagg tgctggtcct gtggctggcg ccgaccgatc
     6961 tggcgtcttt cttcatggcg gtgcccgagc cccccgatgg cgtccgcgtt tacctttccg
     7021 ccagcctcgc cggattcgat acgggcgtcg atacatccct gattcccccc ccgtggcgcc
     7081 cggcggttcg cgttgtctac cccttcgatc tgcccgacct gcggctcggc cgacaggtct
     7141 tcaatctagg cggctggatg gcgggtcacg ggttggccct cgtgcggagt acggcacggg
     7201 ttcagggcga ttcattctcc gcctgcgagg tggtggcgcg ggcgctctac accctgcctg
     7261 aacatccgac ccgcgaagtg ctgctcgaac gggtggagga agcctacgca ggcgcggtgg
     7321 cgacggccta cccccgcttc accctcgggc ccgggcaacg attcggttcc aaaggtgcct
     7381 acctcctgaa aataatgcca gatggaaaat ggcagcctca aggcgcgtgg cgcgtgcctt
     7441 gaagggactg gtttctcggc cattgcgccg atcatcggcg gccgcccgaa aggcgcgccg
     7501 tgtaacgagt ccttgccatc ccggggcgca tcccggttga tctgtgtcaa aatgtcgctc
     7561 tgatcgcggc gtatccttcc gcggaagcct gccggccatg cgtcggcagt caggggggtt
     7621 tttgtttttt cttaatgagg tgactcacat gcaatcgcag acttacaact ataaagtcgt
     7681 gcggcagttc
//
LOCUS       DAOQPA010000143         7517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5471_length_7517_cov_17.406861, whole genome shotgun sequence.
ACCESSION   DAOQPA010000143 DAOQPA010000000
VERSION     DAOQPA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5471_length_7517_cov_17.406861"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..654)
                     /locus_tag="PKX01_15045"
     CDS             complement(91..654)
                     /locus_tag="PKX01_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HMZ77499.1"
                     /translation="MTMSTTPEQFVALNKANVETLLTLANTAFASAERLAALNLNTAR
                     AVLEDSVANAKSLLSAKDVQELVNLQAALLQPVVEKAVAYSRSVYEIASQTQEEVSKL
                     FEAQVAEANKNLAAALDKAAKSAPAGSDVAVAAVKSAIAAANSAYDSVTKAAKQVAEI
                     AEANVAAATSATVKAVSAPAKSSKKAA"
     gene            1137..2171
                     /gene="pyrC"
                     /locus_tag="PKX01_15050"
     CDS             1137..2171
                     /gene="pyrC"
                     /locus_tag="PKX01_15050"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ77500.1"
                     /translation="MSRLTLTRPDDWHLHVRDGGALAAVVPDTARRFGRALIMPNLKP
                     PVTSVAQAAAYRERILAAVPAGLKFAPLMTLYLTDTLPPDEIDRAAASGFIPAAKLYP
                     AGATTNSDAGVTDIAKVYPVLERMAEAGIVLCVHGEVTHADVDIFDRERVFIDRVLAP
                     LATRFPSLRIVFEHITTADAAQFVTDAGPNVGATITAHHLLLNRNAIFTGGIRPHHYC
                     LPVLKRETHREALVKVATSGSAKFFLGTDSAPHARSTKEAACGCAGCYTAHAGIELYA
                     EAFEQAGALDRLEAFASFHGPDFYRLPRNADTITLVKDAWDVPAALDYLPGDPLVPLR
                     GGEQIGWKLA"
     gene            2186..2626
                     /locus_tag="PKX01_15055"
     CDS             2186..2626
                     /locus_tag="PKX01_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77501.1"
                     /translation="MRLRALFLLAAVPFLAACENEGIAYLIDGKDHSIALMREQKWFW
                     SDEVDQAIVVSRMPTCLRRYDIKSGVAGSVKMEVYEAGDYLWALKQGKNWYLAGTEKC
                     EFQRWKDAPETPPGKLVGTFTRKDGRLQFVPADAAPKPIKPAEE"
     gene            2626..3459
                     /locus_tag="PKX01_15060"
     CDS             2626..3459
                     /locus_tag="PKX01_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HMZ77502.1"
                     /translation="MPPAAPAALAARPLFEPLRGLLEAFRDPVLPDAAALTALLHAHA
                     PGAVSGSGRPIRFVLPPPGDTAGYEAHIHATGEVPTRRDDWHDFFNALAWAVWPRTKS
                     VCNAQHLLEIARRQAAGKPGRGPVRDALTQLDECGVIVVTCDPALPARLADHQWEAVF
                     VTRRAQLATTTRFLVLGHATWDQLRAPFVGLCGKALFRCVEPAWFALDPFAQQAECDA
                     WLAESLVARAASLTPRTWSPLPLLGIPGVTPESEDPAYYRDTRQFRAKRRATSFCQYE
                     R"
     gene            3526..3801
                     /locus_tag="PKX01_15065"
     CDS             3526..3801
                     /locus_tag="PKX01_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ77503.1"
                     /translation="MRTLTSVEAQNRFGELIDSAQREPVSITRRGRTVAFVLSPADMQ
                     ALLAVRQQRERVVADFEAFFSESDARLDPQADPLSDADIQRLVNELR"
     gene            3788..4207
                     /locus_tag="PKX01_15070"
     CDS             3788..4207
                     /locus_tag="PKX01_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ77504.1"
                     /translation="MNCVERVVFDTSTLIGAMLRPTSIPRQAFLKAVRQAELCASPAT
                     LAELEVVLLRDKFDRYLGREHRLQFLELYRRHARLFAVSDADETEMPCRDPRDNKFLA
                     LALACSANAIVSSDDDLQVLNPFREIPILLPRDYLDR"
     gene            4409..6049
                     /gene="gpmI"
                     /locus_tag="PKX01_15075"
     CDS             4409..6049
                     /gene="gpmI"
                     /locus_tag="PKX01_15075"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMZ77505.1"
                     /translation="MLQKLPAFPGVAGPVVVIVLDGYGLPKSDVGSAIAAARKPTLDR
                     LFAECPHIRLRAHGTAVGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVSNAIADGAIW
                     QGEAWQQIIAGARAGRGVLHFIGLFSDGNVHSHIDHLKAMVIRAKAEGIPTVRIHALL
                     DGRDVPETSALDYVVPFEAFLAELSSGGFDARIASGGGRQAITMDRYDANWPMVARGW
                     RTHVLGEGPQFANATAAVHGLRERHPGTIDQDLPEFVIADAGQPIGTIEDGDAVVFYN
                     FRGDRAIEITRAFVDESFDKFDRVRTPQVTYAGMLQYDGDLQLPKRFLVAPPAIKDTS
                     GEWFSRLGLTQFACSETQKFGHVTYFWNGNRSGKFEGETWQEVPSDVVPFEQRPWMKA
                     AEITDAMIAAVQSGQYALLRCNYANGDMVGHTGNFRAATMAVEAVDLALARLLPAIQA
                     AGGVALITADHGNADEMFELDKKTKQPALNADGSYKAKTAHTLNPVPLVLFDKVTGGR
                     LGLQQTDTAGLSNIAATVANLLGYAKHPAWDDSVLTID"
     gene            6186..6533
                     /locus_tag="PKX01_15080"
     CDS             6186..6533
                     /locus_tag="PKX01_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ77506.1"
                     /translation="MPTDPAAAMNLEGVEACHWQDVPPRPLEHPGFSTRLLWRGPDGR
                     RALIFEIAPGAVYPELDVHLPGAEEVFVVSGSFCDGRNTYPAGTFIHHPAGSSHVPQS
                     EEGCVLFVYFPEG"
     gene            6747..6968
                     /locus_tag="PKX01_15085"
     CDS             6747..6968
                     /locus_tag="PKX01_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77507.1"
                     /translation="MKTVSVTDLRRKPIETALAIAAAVQGGQGLEITRRGRVVEVIGA
                     DRPGKDEVARQLDVMRRADATDDFADFAE"
     gene            6965..7288
                     /locus_tag="PKX01_15090"
     CDS             6965..7288
                     /locus_tag="PKX01_15090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMZ77508.1"
                     /translation="MKRGELYWVASFKDFGKVRPAVVVQADAFNENPTSVTVCLLTGE
                     RVDAPLFRLDVPADTETGLDKPSQIMADKLITVPVARIKEPIGKIPARTLSKLDDSLR
                     LGRSL"
BASE COUNT         1218 a         2800 c         2251 g         1248 t
ORIGIN      
        1 gccccaaaac aaacatggcc cgcaccgttg ccggtggggc catgtcgcga tcaagaccgc
       61 gccggagcgc gatccggatc cgggtgaaac ttaggcagcc ttcttggaag acttggccgg
      121 ggcgctgacg gccttgacgg tggcgctggt ggcagcggca acgttggctt cggcgatttc
      181 ggcgacttgc ttggcagcct tggtcacgct gtcgtaggcg gagttggcgg cggcgatggc
      241 ggacttcacg gcagccacgg cgacgtcgga accggcggga gcggacttgg cagccttgtc
      301 gagggcggca gccaggttct tgttggcttc agcgacttgg gcttcgaaca gcttggaaac
      361 ttcttcctgg gtctgggaag cgatctcgta gacgctgcgg gagtaggcca cggccttttc
      421 cacgacgggc tgcagcaggg cggcttgcag gttcaccagc tcttgcacgt ccttggcgga
      481 cagcagggac ttggcgttgg caacgctgtc ttccagcacg gcgcgggcgg tgttcaggtt
      541 cagggcggcg agacgctcgg cgctggcgaa ggcggtgttg gccagggtca gcagggtttc
      601 gacgttggcc ttgttgaggg cgacgaattg ctcgggagtg gtgctcatgg tgatctccta
      661 aaggtaaaaa gggtgattac gttctgtgtc ggggcgttgc tttattgcgc tgcaacatgg
      721 gttccattat atgcaccgaa agagtgctgt caacactttt ttgttgcgcc gcacaaaaat
      781 atttgcagcc tgtatttccg tttcaaaaca atggggttaa cacgctgacg aggtgccatc
      841 cggatagcag gcgtgagtcg cccgaaatgc agcaggcacg ccgatttcgg gcgtttcggg
      901 ggcttccgga agcaggctcg gagacccccg cgacgcccgc gctgcactgc tttggaccgt
      961 ccggactgcc ctgccatgtc gccgggagaa atcgatgcat ctggcacctg atcccgcgcc
     1021 ttccgatcgg cggcgcagcc gtttttcagg gtcttcgccc cgccggtggc gcctccgcgg
     1081 ggtgggggcg ctgttaaaat gcgcattccc gtcattcccc agcccggatc tttcccatgt
     1141 cccgcctgac cctcacccgc cccgacgact ggcatcttca tgtgcgcgac ggcggcgccc
     1201 tcgcggccgt cgtgcccgac accgcgcgcc gcttcggccg cgcgctgatc atgcccaacc
     1261 tcaagccgcc ggtgacgagc gtggcgcagg ccgccgcgta tcgcgaacgc atcctcgcgg
     1321 ccgtgcccgc ggggctcaag ttcgccccgc tgatgacgtt gtacctcacc gacacccttc
     1381 cgcccgacga gatcgaccgg gctgccgcga gcggcttcat ccccgccgcc aagctctacc
     1441 cggccggcgc caccaccaac tcggacgcgg gcgtcaccga catcgccaag gtctatcccg
     1501 tcctcgaacg gatggccgaa gcgggcatcg ttctctgtgt tcatggcgag gtgacccatg
     1561 ctgacgtgga tatctttgat cgcgagcgcg tctttatcga tcgcgtgctg gcgccgcttg
     1621 caacgcgctt cccgtccctg cgcatcgtct tcgagcacat cacgacggcc gatgcggcgc
     1681 agttcgtgac ggacgccggg cccaacgtgg gcgccacgat caccgcccac catctgctgc
     1741 tcaaccgcaa cgcgatcttc accggcggca tccggcccca tcactactgc ctgccggtgc
     1801 tcaagcgcga aacccatcgc gaggccctcg tgaaggtcgc cacctccggc agcgccaaat
     1861 tcttcctcgg caccgactcc gcgccccacg cccgcagcac caaggaagcc gcctgcggtt
     1921 gcgccggctg ctacaccgcc cacgccggca tcgagctcta cgccgaagcc ttcgagcagg
     1981 ccggcgcgct ggatcgactg gaagccttcg ccagcttcca cggcccggat ttctaccgcc
     2041 tgccccgcaa cgccgacacg atcaccctgg tcaaggacgc ctgggacgtg cccgccgccc
     2101 tcgactacct gcccggcgat cccctcgtgc ccctgcgcgg gggcgagcag atcggctgga
     2161 agctcgcctg acatccgcca ccacgatgcg cctgcgtgcc ctgttcctcc tcgccgccgt
     2221 gcccttcctc gctgcctgcg agaacgaggg catcgcctac ctcatcgacg gcaaggacca
     2281 ttccatcgcc ctgatgcgcg aacagaagtg gttctggagc gacgaggtgg atcaggccat
     2341 cgtcgtctcg cgcatgccca cctgcctgcg ccgctacgac atcaagtccg gcgtcgccgg
     2401 cagcgtcaag atggaagtgt acgaagcggg cgactacctg tgggccctca agcagggcaa
     2461 gaactggtac ctcgccggca ccgagaagtg cgagttccag cgctggaagg acgcccccga
     2521 gacgccgccc ggcaaactcg tcggcacctt cacgcgcaag gacggccgtc tccagttcgt
     2581 gcccgccgac gccgcgccca agccaatcaa acccgccgaa gagtgatccc gcccgccgcg
     2641 ccggccgccc tggcggcgcg tcccctgttc gagcccctgc gcggcctgct cgaagccttc
     2701 cgcgatcccg ttctgcccga cgctgcagcc ctcaccgccc tgctgcacgc tcacgcgccg
     2761 ggcgccgtca gcggcagcgg ccggccgatc cgcttcgtcc tgccgccgcc gggtgacacc
     2821 gccggctacg aagcccacat ccacgccacc ggcgaagtcc caacccgccg ggacgactgg
     2881 cacgacttct tcaacgccct cgcctgggcc gtgtggccac gcaccaagtc cgtctgcaac
     2941 gcccagcacc tgctggagat cgcccgccgt caggctgccg gcaaacccgg ccgcggcccc
     3001 gtccgcgacg ccctcaccca gctcgacgag tgcggtgtga tcgtcgtcac ctgcgacccg
     3061 gcgctacccg cgcgcctcgc cgaccatcaa tgggaggccg tcttcgtcac ccgccgcgcc
     3121 cagctcgcca ccaccacccg cttcctcgtc ctcggccacg ccacctggga ccagctccgc
     3181 gcgcccttcg tcggcctgtg cggcaaggcc ctgttccgct gtgtcgaacc cgcctggttc
     3241 gccctcgatc ctttcgcgca gcaggccgaa tgcgatgcct ggctcgccga atccctcgtc
     3301 gcccgcgccg catccctcac gccccgcacc tggtcgcccc tgccgctgct gggtatcccc
     3361 ggcgtcacgc ccgagagcga agatccggcg tactaccggg atacgcggca gttcagggcg
     3421 aagcgccggg cgacatcctt ctgccagtac gagcgctaac ggatcgagca tccgcagggg
     3481 tgggctatag tggacaggtt agctatttca gcgaggagca ttcccatgcg gacactcact
     3541 tcggtcgaag cccagaatcg tttcggtgag ttgatcgaca gcgcccaacg cgagccggtc
     3601 agcattaccc gccgtggccg gacggtggcc tttgtgctct cgcccgccga catgcaggcc
     3661 ttacttgccg tccgtcagca gcgtgagcgc gtcgtggcgg atttcgaagc ttttttcagt
     3721 gaatcggatg cccgcctcga ccctcaggcc gatccgcttt ccgacgcgga tattcagcgg
     3781 ttggtgaatg aactgcgttg agcgggtcgt tttcgacacc agcacgctga tcggcgcgat
     3841 gctgcggccc acctccatcc ctcgtcaggc gtttctgaag gccgtccgtc aggccgaact
     3901 ctgcgcctcg cccgcaaccc tggccgaact cgaagtcgtg cttttgcggg acaagttcga
     3961 ccgttacctc ggccgcgagc atcgcctgca attcctggag ctctaccgtc gtcacgcccg
     4021 actgttcgcc gtatcggacg ccgatgagac cgaaatgccc tgtcgcgatc cgcgtgacaa
     4081 caaattcctc gcgcttgcgc tggcgtgttc tgccaacgca attgtcagca gcgatgacga
     4141 tttgcaggtt ctgaacccct tccgcgagat ccccatcctg ctcccccggg actatctgga
     4201 ccgatgacgc gggttcgccg cgctcgcact tcaagggtgt cgcgcgcgag ggagcgatga
     4261 gcccccccac cccggccacc cgtcacgaac cacctgacat aaacctgtct tcaccccctc
     4321 atttgaagga caatagcgcc ctcgccgggg gccgtgggcg atgcgccccg ccggaccggc
     4381 ctcgcgttca tccttcagga agccctccat gctgcagaaa ctccccgcct tccccggcgt
     4441 cgccggtccg gtcgtcgtca tcgtcctcga cggctacggc ctgcccaaat ccgacgtggg
     4501 cagcgccatc gccgccgcgc gcaagcccac cctcgaccgc ctcttcgccg aatgtccgca
     4561 catccgcctg cgcgcccacg gcaccgcggt cggcatgccc tccgacgacg acatgggcaa
     4621 ctccgaagtg ggtcacaacg ccatcggcgc cggccaggtg tatgcccagg gcgctgccct
     4681 cgtctccaat gccatcgccg acggcgccat ctggcagggc gaagcctggc agcagatcat
     4741 cgccggcgcc agggccggtc gcggcgtgct ccacttcatc ggcctgttct ccgacggcaa
     4801 cgtgcacagc cacatcgacc acctcaaggc catggtgatc cgcgccaagg cagaaggcat
     4861 ccccaccgtg cgcatccacg ccctgctcga tggccgcgac gtgcccgaaa ccagcgcgct
     4921 ggactacgtc gtgcccttcg aagccttcct cgccgagctc agcagcggcg gcttcgacgc
     4981 ccgcatcgcc tccggcggcg gccgtcaggc catcaccatg gaccgctacg acgccaactg
     5041 gcccatggtg gcccgcggct ggcgcaccca cgtcctcggc gaagggcccc agttcgccaa
     5101 cgccaccgct gccgtccacg gcctgcgcga acgccatccc ggtaccatcg accaggacct
     5161 gcccgagttc gtcatcgccg acgccggcca gcccatcggc accatcgaag acggcgacgc
     5221 cgtcgtcttc tacaacttcc gcggcgaccg ggccatcgag atcacccgcg ccttcgtcga
     5281 cgaaagcttc gacaagttcg accgcgtgcg caccccgcag gtcacctacg ccggcatgct
     5341 ccagtacgac ggcgacctgc aactgcccaa gcgcttcctc gtcgccccgc ccgccatcaa
     5401 ggacacctcc ggtgaatggt tcagccgcct cggcctcacc cagttcgcct gctccgagac
     5461 ccagaagttc ggccacgtca cctacttctg gaacggcaac cgctccggca agttcgaagg
     5521 cgaaacctgg caggaagtcc ccagcgacgt ggtgcccttc gagcagcgcc cgtggatgaa
     5581 ggccgccgaa atcaccgacg ccatgatcgc cgcggtccag tccggccagt acgcactgct
     5641 ccgctgcaac tacgccaacg gcgacatggt cggccacacc ggcaacttcc gcgccgccac
     5701 catggccgtc gaggctgtcg atctcgccct cgcgcgcctg ctccccgcca tccaggctgc
     5761 gggcggtgtc gccctcatca ccgccgacca cggcaacgcc gacgagatgt tcgagctgga
     5821 caaaaagacc aaacagcccg ccctcaacgc cgacggctcc tacaaggcca agaccgccca
     5881 cacgctgaac cccgtgcccc tcgtcctctt cgacaaagtc accggcggcc gcctcggcct
     5941 gcagcagacg gacaccgccg gcctctccaa catcgccgcc accgtcgcca acctcctcgg
     6001 ctacgccaag catcccgcct gggacgacag cgtcctcacc atcgactgat ccgcggtggt
     6061 atgcgcgggc ggcctgcggg gcgcccgccc tctgtgggcg tcgcggccac cgccgcggcg
     6121 ttccgcgacg gggtgatcca cctcaccgcg cgccgcgtgt ctttcaatcc tgcctgagag
     6181 agccaatgcc caccgatcct gccgccgcga tgaacctcga aggcgtcgaa gcctgccact
     6241 ggcaggacgt ccccccgcgc ccactggaac accccggctt cagcacccgt ctgctgtggc
     6301 gcggcccgga tgggcggcgg gcgctgatct tcgagatcgc ccccggcgcc gtctatcccg
     6361 agctggacgt gcatctgccc ggcgccgagg aagtcttcgt cgtctccggc agcttctgcg
     6421 acggccgcaa cacctatccc gccggcacct tcatccatca cccggccggc tcgtcccacg
     6481 tgccccagtc ggaggaaggc tgcgtgctgt tcgtgtattt tccggaaggt tgagacggcg
     6541 ttatcggtcg cggggccgcc gtcatggcgc ccgaccggaa cgcctcccgc tgcacgtgcc
     6601 cctttcaatc ccccgcgacg ggttggtgca agtcgccccg gcgcatccga tggcggcgaa
     6661 cgccccgcag acgggggcat gtccttccgg gcgcgcgcat cccggcacgt ggggtgcatc
     6721 attccgtcag cggaagggga aagcccatga agaccgtctc tgtcaccgac ttgcgccgca
     6781 aacccatcga aaccgccctg gccattgcgg ccgcagtgca gggcggacag gggctcgaaa
     6841 tcacccgtcg ggggcgcgtg gtggaggtca tcggcgccga ccggccgggg aaggatgaag
     6901 tggcgcgcca gcttgacgtg atgcgccggg ccgacgccac tgacgacttc gccgactttg
     6961 ccgaatgaag cgcggcgaac tctactgggt ggccagcttc aaggacttcg gcaaggtccg
     7021 tccggccgtc gtggtccagg ccgatgcctt caacgaaaat cccaccagcg tgactgtctg
     7081 cctgctgacc ggagaacggg tggatgcgcc gctgtttcga ctcgacgtac cggccgacac
     7141 ggaaaccggc ctcgacaagc cgtcgcagat catggcggac aaactgatca ccgtgccggt
     7201 cgcccgcatc aaggaaccca tcggcaagat tcccgcacgc accctgtcca aattggacga
     7261 ttcactccgc ctcgggcggt ccctttaaag aatccgggcg cgatgcccgg acatgttctg
     7321 cacgtgaaag ccttgtcgta gacgcctctg ccaatggggg ccgctggcga tcagggcccg
     7381 cgctgccttg gcccgcgtct cagatctgcg cggaagagac ctgcacacca aaaacaggcc
     7441 agcctccgct cactgggaag acgtctccgc cgccgccttc atctcctccc cgaactcctg
     7501 ctggatcttc agcagtg
//
LOCUS       DAOQPA010000144         7341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5680_length_7341_cov_20.262696, whole genome shotgun sequence.
ACCESSION   DAOQPA010000144 DAOQPA010000000
VERSION     DAOQPA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5680_length_7341_cov_20.262696"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKX01_15095"
     CDS             complement(<1..494)
                     /locus_tag="PKX01_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HMZ77509.1"
                     /translation="MRIAFIVDPLDGLKAYKDSSIAMMRAAARRGHQVFAIRRADLVW
                     EAGTVSARALALTPTDDDHAWYVPGAEAPMALKAFDAVLMRQDPPFDFEYVAATWLLE
                     RAAAAGARVFNAPRAIRDHSEKLAITEFPQFIPPTRVARAAVDVHAFIDAHGDTILKP
                     LDGMG"
     gene            complement(504..1802)
                     /gene="gshA"
                     /locus_tag="PKX01_15100"
     CDS             complement(504..1802)
                     /gene="gshA"
                     /locus_tag="PKX01_15100"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HMZ77510.1"
                     /translation="MVPHLTTALTGPLLELERHFLDNACAIERWMRTQWQEHLPPFYG
                     STDLRNSGFKLAPVDLNLFPGGFNNLNDAFMPLCVQAAQAAIERICVGARAILLIPEN
                     HTRNQFYLQNVAKLVSILRLTGLEVRLGSLLPEITAPTVIELANGASLTLEPLERHGR
                     RLGVKGFDPCAVLLNNDLSAGIPDQLRDLHDQWVIPPVHAGWHTRRKSHHARAYDRVV
                     ADFSDAIGIDPWRINPAHDTCHGLDFHARTGEQELADKVAAMLDRIRAKYREYGVSDE
                     PFVVVKADAGTYGMGVMTVKDASELIGLNRKQRNKMSVVKEGLEVHDVIIQEGVHTFE
                     TVHGAVAEPVVYMMDHYVVGGFYRVHTQRGRDENLNAPGMHFEPLAFEAPCSLPDCNQ
                     DPDAPPNRFYAYGVVARLALLAAALEIEETEPAVLEDAVA"
     gene            complement(1928..3154)
                     /locus_tag="PKX01_15105"
     CDS             complement(1928..3154)
                     /locus_tag="PKX01_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ77511.1"
                     /translation="MSAPAPLDVAVLGQVFTPEIVVRAMLRLRHNTGRVLEPACGDGA
                     FLRHLKQVTGIEVDAAHCPPGARRMDFFAFPENEKFDTVIGNPPYVRYQDIPASTRSL
                     LRHDGFDGRSNLYLFFIAKCVRHLAPGGELIFITPRDFLKSTSARQLNRWLYRQGTIT
                     HAIELGDSKVFADAVPNCLIWRFELGNLSHHTAWMAVRQGEALATALESPHWHYRHFS
                     EFAGQLHFSRGEYALSLADVASVRVGAVSGLDELYASDTHGNRDFVSSATARTGLTRR
                     MIWCEPGDPPPAALLPYRDRLLARRIRRFDASNWWQWGRSYPQTDAPRVYVNGKTRAE
                     QPFFLHPCPHFDGAVMGVFPHRPDVDLGAFRDALNRVNWDDLGFVCDGRHLFTQRSLE
                     NAPLPAAFRAFLPDTP"
     gene            3455..4270
                     /locus_tag="PKX01_15110"
     CDS             3455..4270
                     /locus_tag="PKX01_15110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ77512.1"
                     /translation="MRELSLADVLAGRSPDQLSAAELAAGRDGVLIRCMPDRGTRTLT
                     LEGDRLQYVVAREHWHYLAMAGSFEQYLADRFSEKTRASFERDEGRFVETFGRLDLRE
                     YRSPEELQRFALIAAQVVDGRDRMPALGAMQPGDDAFALFADGAPVAFLHLRSAGDTV
                     QYHSAGESEAFRQWSAGSVLHVAVLRRLFADGRFRYLDFMPGDGEIKRQFANGALRCS
                     DILYLRPTLTNHLLLRLHAYLGRCTAWLDGQAGRAGVVGLLHRLFSREARQVG"
     gene            complement(4289..5503)
                     /gene="hemW"
                     /locus_tag="PKX01_15115"
     CDS             complement(4289..5503)
                     /gene="hemW"
                     /locus_tag="PKX01_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMZ77513.1"
                     /translation="MTERRIIPIAIQAAPPAGPRVPQLTASPPLALYIHFPWCVRKCP
                     YCDFNSHAVRDEGIPEAAYLEALIADLESALPGIWGRRVHSVFIGGGTPSLLSPDGLD
                     ALLTAVRMRVLLDPMAEITLEANPGTVEAGRFRAFREAGVNRLSLGIQSFDDRHLQAL
                     GRIHGSAEARAAAEIALAHFDTVNLDLMYALPGQTLADAEADLATALALGPAHLSCYH
                     LTLEPNTAFAAHPPELPDGDLAADMQDHVESRLSAAGYVHYETSAFARPQRECRHNLN
                     YWTFGDYLGIGAGAHGKLSSHAGILREMRHKHPAAYLAAQQSGDFVQERRTVSGAELP
                     FEFMMNALRLNGGFPLRLFAERTGLPSEAAETGLIEARRRDLVTIEDDWLRPTDQGRR
                     FLNDLLTLFLAD"
     gene            complement(5538..6131)
                     /gene="rdgB"
                     /locus_tag="PKX01_15120"
     CDS             complement(5538..6131)
                     /gene="rdgB"
                     /locus_tag="PKX01_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMZ77514.1"
                     /translation="MKQLVLASGNAGKLKELNALLAPLGIEVLPQSAFHVSEAEEPHP
                     TFVENALAKARHASRATGLPALADDSGLCVSALGGAPGVMSARFAGEPKSDARNNSLL
                     LERLDGASDRRARFVCTLALVHHADDPLPLIATGEWHGEILAEARGQSGFGYDPLFYV
                     PDLDQTAAELAPELKNILSHRGAAFRQLLEKLRAELA"
     gene            complement(6153..6890)
                     /gene="rph"
                     /locus_tag="PKX01_15125"
     CDS             complement(6153..6890)
                     /gene="rph"
                     /locus_tag="PKX01_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HMZ77515.1"
                     /translation="MTDTSFARPSGRAPGELRSVRLTRHFTRHAEGSVLVEFGDTRVL
                     CTASVEESVPPFLRGKGQGWLTAEYGMLPRSTHTRSPREAAKGKQSGRTQEIQRLIGR
                     SLRAVVDLQALGERQITLDCDVLQADGGTRTASITGACVAAWDALAGLVAAGKLTAHP
                     MRDFVAAISVGIYRGVPVLDLDYPEDSDCETDMNVVMTGSGGFVEVQGTAEGAPFSRP
                     ELDALLELAGGGVAQLVREQKKALGLA"
     gene            complement(6887..>7341)
                     /locus_tag="PKX01_15130"
     CDS             complement(6887..>7341)
                     /locus_tag="PKX01_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HMZ77516.1"
                     /translation="AAASHPGRNRIFSCLGGNHPPQIEFSRAVTLANGDVIAMCSDGV
                     WGPIDTDSLVRTLSNGRVMEGVPALMDLSEAAGGAQCDNMSLIAVAWHDELQEDSPET
                     VSTRGMPADAYTTQMEGFGRRRPDQEDLSEDEIEKAIREINAAIHKFK"
BASE COUNT         1137 a         2173 c         2787 g         1244 t
ORIGIN      
        1 cccatgccgt cgaggggctt gaggatggtg tcgccgtggg cgtcgatgaa ggcgtgtacg
       61 tctaccgcgg cgcgggccac gcgggtgggc gggatgaact ggggaaactc ggtgatggcg
      121 agcttctcgg aatggtcgcg gatcgcccgc ggggcgttga agacgcgggc gccggctgcg
      181 gcggcgcgtt ccagcagcca ggtggcggcg acgtattcga agtcgaaggg cggatcctgg
      241 cgcatcagca ccgcgtcgaa ggccttgagc gccatcggcg cctcggcgcc ggggacgtac
      301 caggcgtggt cgtcatcggt gggggtgagg gccagggcac gggcggagac ggtgcccgcc
      361 tcccatacca ggtcggcgcg ccggatcgcg aacacctggt ggccgcggcg ggcggcggcc
      421 cgcatcatgg cgatgctgga atccttgtag gccttgaggc cgtccagggg gtcgacgatg
      481 aaggcgatgc gcatatgggg gccttatgcg accgcgtctt cgaggacggc cggctcggtt
      541 tcctcgatct ccagcgcggc ggccagcagg gcgaggcggg cgaccacgcc gtaggcgtag
      601 aagcgattgg gcggggcgtc gggatcctgg ttgcagtcgg gcaggctgca gggcgcttcg
      661 aaggcgaggg gctcgaagtg catgcccggc gcgttgaggt tctcgtcgcg gccgcgctgg
      721 gtgtgaaccc ggtagaaccc ccccacgacg tagtggtcca tcatgtagac gaccggctcg
      781 gcgacggcgc cgtggacggt ctcgaaggtg tgcacgcctt cctggatgat gacatcgtgg
      841 acttccaggc cttccttgac gacgctcatc ttgttgcgct gcttgcggtt gaggccgatc
      901 agttcggacg cgtccttgac cgtcatgacg cccatgccat aggtgccggc gtcggccttg
      961 acgaccacga agggctcgtc gctgacgccg tattcccggt acttggcgcg gatgcggtcg
     1021 agcatggcgg cgaccttgtc ggccagttcc tgctcgccgg tgcgggcgtg gaagtcgagg
     1081 ccgtggcagg tgtcatgggc cggattgatg cgccacgggt cgatgccgat cgcgtcggag
     1141 aagtcggcca cgacgcggtc gtaggcgcgg gcgtggtggg atttgcggcg ggtgtgccag
     1201 ccagcatgga ccggcgggat cacccactgg tcgtggaggt cacggagctg gtccgggatg
     1261 ccggcggaga ggtcattgtt gaggagcacc gcgcacggat cgaaaccctt caccccgagg
     1321 cggcggccgt ggcgttccag gggctccagg gtgaggctcg cgccgttggc cagttcgatc
     1381 acggtggggg cggtgatctc cggcagcagg ctgccaaggc gcacttccag gccggtgagg
     1441 cgcaggatgg agaccagctt ggcgacgttc tgcaggtaga actggttgcg ggtgtggttc
     1501 tcggggatca gcaggatggc gcgggcgccg acgcagatgc gctcgatggc ggcctgggca
     1561 gcctgcacgc agaggggcat gaaggcgtcg ttgaggttgt tgaagccgcc ggggaagagg
     1621 ttgaggtcca ccggggcgag cttgaagccc gagttgcgca ggtcggtgga gccgtagaag
     1681 ggcggcaggt gctcctgcca ttgggtacgc atccagcgtt cgatggcgca ggcgttgtcg
     1741 aggaagtgcc gctccagctc cagcaggggg ccggtgagcg cggtagtgag atggggaacc
     1801 atggaacctc gcgggctgtt ctcgggtggg cggcgcgttt cggtgcgtcg acagggatgt
     1861 ccggcgtgga ccggcgtcgc ccggaattgt tcggcggatg ttaccacctc cgccggaatg
     1921 cctttgttta cggggtgtcc ggcaggaagg cgcggaaggc ggcggggagc ggggcgttct
     1981 cgagactgcg ctgggtgaac aggtgacgtc cgtcgcagac gaagcccagg tcgtcccagt
     2041 tcacccggtt gagggcgtcc cggaaggcgc ccagatccac gtccggacgg tgcggaaaca
     2101 cgcccatcac ggcaccatcg aagtgggggc agggatgcag gaaaaagggc tgctccgcgc
     2161 gggtcttgcc attgacgtag actcgcggag cgtcggtctg gggatagctg cgcccccact
     2221 gccaccagtt ggacgcatcg aatcggcgga tgcgccgggc aaggaggcgg tcccggtagg
     2281 gcaacagtgc ggcaggcggc gggtcgcccg gctcgcacca gatcatgcgc cgggtcaggc
     2341 ccgtgcgggc ggtggcagat gagacgaagt cccggttgcc gtgggtgtca ctggcgtaga
     2401 gctcatccag gcccgatacc gccccaaccc gcacgctggc cacgtccgcc agactgagcg
     2461 cgtactcccc ccgggaaaaa tgcagctggc cggcaaattc ggagaagtga cggtagtgcc
     2521 agtggggcga ttcgagggct gtggccaggg cctccccctg ccgcaccgcc atccaggccg
     2581 tgtggtggga caggttgccc aattcgaagc gccagatcag gcaattgggg accgcgtcgg
     2641 cgaacacctt gctgtcgccc agttcaatgg cgtgcgtgat ggtgccctgg cggtacagcc
     2701 agcgattgag ctggcgggca ctggtggatt tgaggaaatc ccgcggcgtg atgaagatca
     2761 gctcgcctcc gggcgccagg tggcgcacgc acttggcgat gaagaagaga tacaggttgg
     2821 aacgcccgtc gaagccgtca tggcgcagca ggctgcgggt ggacgcgggg atgtcctggt
     2881 accgcacgta gggcgggttg ccgatcacgg tgtcgaattt ctcgttttcc ggaaaggcga
     2941 agaaatccat gcggcgtgcg cccggcggac agtgggcagc atccacctcg atgcccgtga
     3001 cctgtttgag atggcgcagg aaggcgccgt cgccacaggc gggttcgagc acccgtccgg
     3061 tgttgtgacg caggcgaagc atggcgcgca ccacgatttc cggcgtaaac acctggccga
     3121 gcacggcgac atcaaggggg gcaggggcgg acatgggcgg gtgagtcgga ataatcctcc
     3181 attttgcccc aatgatcggc ccgctgcagg ggggctgcca ggccgccctg cgggcgggcg
     3241 ggcccgaatt gtgccaatgc gtggcaacgg tacgacgaaa gtatgatttg taatgatatc
     3301 gtgctatctt tcaaattgtt aaagatcatg actggctgcc tctctcgcgg ccttttgcgg
     3361 gtgcatcaaa atgactgagc agggtgaaac agaacatcgc ctgacatgcg gactgggtcg
     3421 gcgggtcttg ttctcggtgg caaggcgcgt ggccgtgcgg gagctttcgc tggcggacgt
     3481 gctggcaggc cggagtccgg accaattgtc tgctgccgag ctcgcggcgg gtcgcgacgg
     3541 agtcctcatt cgctgcatgc ccgaccgggg gacgcggacg ctcactctgg aaggcgaccg
     3601 gctgcaatac gtggtggcgc gcgagcactg gcactacctg gcgatggccg gcagcttcga
     3661 gcagtacctt gcggatcgct tctccgaaaa gacccgggcg tccttcgagc gggacgaggg
     3721 gcgctttgtc gagacctttg gtcgcctcga tttgcgggaa taccgcagtc cggaagagct
     3781 tcaacggttt gcgctgatcg cggcccaggt tgtggatggc cgcgaccgca tgccggccct
     3841 gggggccatg cagccggggg acgatgcttt cgccctgttt gccgatgggg cgccggtggc
     3901 gttcctgcac ctgcgttcgg ctggcgacac ggttcagtat cacagcgcgg gtgaaagcga
     3961 ggccttccgc caatggtccg ccggctcggt gctccacgtg gcagtcctgc gccgcctgtt
     4021 cgccgacgga agattccgct atctggactt catgccgggc gacggggaga tcaaacgtca
     4081 gttcgccaat ggcgccctgc gctgttccga catcctctac ctgcgcccca ccctcacaaa
     4141 ccacctgctc ctgcgcctgc atgcctatct gggacggtgc accgcgtggc tggatgggca
     4201 ggccggacgg gcgggggtcg tcgggctcct gcatcgcctg ttcagccgcg aagcccgcca
     4261 ggtcggctga ggcgacttct gcggccggtc agtccgccag gaacagggtc agcaggtcat
     4321 tgaggaatcg ccgcccctga tcggtgggac gtagccagtc gtcctcgatg gtcaccaggt
     4381 cgcggcgccg ggcctcgatc aggccggtct cggcggcctc ggagggcagt ccggtgcgct
     4441 ctgcgaacag ccgcagcgga aagcccccat tcaggcgcag ggcgttcatc atgaattcga
     4501 agggcagttc agcgccgctc acggtgcgcc gttcctgtac gaaatcgcct gattgttgcg
     4561 cggctaggta ggcggcgggg tgcttgtgac gcatctcgcg caggatgcct gcgtgggacg
     4621 agagcttgcc gtgggcaccg gcgccaatcc ccagataatc cccaaatgtc cagtaattga
     4681 ggttgtggcg gcattcccgt tgagggcggg caaaggcgga cgtttcgtag tggacgtagc
     4741 ccgcagcgct cagacgcgat tccacgtggt cctgcatgtc ggccgcaagg tctccgtcgg
     4801 gtagttccgg cgggtgggcg gcgaacgccg tgttgggttc cagcgtgagg tgatagcacg
     4861 acaggtgcgc cggtccgagg gcgagggcgg tggccaggtc ggcctcggcg tcggccaggg
     4921 tctggccggg cagcgcgtac atgaggtcga ggttgaccgt gtcgaaatgg gcgagggcga
     4981 tctccgctgc ggccctcgcc tcggcgctcc cgtggatgcg tcccagggcc tgcagatggc
     5041 ggtcgtcgaa gctctggatg cccagggaga ggcgattgac gccggcttcg cgaaacgcgc
     5101 ggaagcgtcc ggcctcgacg gtgcccggat tggcctccag cgtgatctcg gccatggggt
     5161 cgaggagcac ccgcatgcgg accgcggtga gcagggcgtc gagcccatcc gggctgagca
     5221 ggctgggggt gccgcccccg atgaacacgc tgtgcacccg gcgcccccag atgcccggca
     5281 gcgcactttc cagatcagcg atcagggcct cgagataggc cgcttccggg atgccttcgt
     5341 cccgtacggc gtgggaattg aagtcgcagt atggacactt gcgcacgcac cacgggaagt
     5401 ggatgtacag cgccaggggc ggcgaggcgg tgagttgcgg cacccgcggc ccggcaggcg
     5461 gggcggcttg gatggcaatc gggatgatgc ggcgttcggt catggcgcgg gcggcgccgg
     5521 aggagcgccg cgagggttca ggcgagttca gcgcgcagct tttcgaggag ctgacggaag
     5581 gcggcgcctc ggtgggagag gatgttcttc agttcgggcg ccagttcggc ggcggtctgg
     5641 tccagatcgg gcacgtagaa cagagggtcg tagccgaagc cggactggcc gcgggcttcg
     5701 gcgaggatct cgccgtgcca ctcgccggtg gcgatgaggg gcaaggggtc atccgcatga
     5761 tgtacaaggg cgagggtgca tacgaagcgg gcacggcggt cgctggcgcc gtcgaggcgc
     5821 tccagcagca ggctattgtt gcgggcgtcg gatttgggtt cgccggcgaa gcgcgcggac
     5881 atcacgcctg gcgcgccacc gagggcgctc acgcagaggc cggagtcgtc ggcgagggcc
     5941 ggcaggcccg tggcgcggga tgcgtgacgg gccttggcaa gcgcattttc gacgaacgtg
     6001 gggtgaggct cttccgcttc gctcacatgg aaggcggact ggggcagcac ctcgatgccc
     6061 agcggggcga gcagcgcatt gagttccttg agtttgccgg cgttgccgct ggcaaggacg
     6121 agttgcttca tggtcggcct gctgcgcgcc gctcaggcga gcccgagcgc ctttttctgt
     6181 tcacggacca gttgggccac accgccgccg gcaagttcca gcagggcatc cagttcgggc
     6241 cgggagaagg gtgccccttc agcggtcccc tggacctcga cgaagccccc gctgccggtc
     6301 atgaccacgt tcatgtcggt ctcgcagtcg gagtcttccg ggtagtccag gtcgaggaca
     6361 ggcaccccgc ggtagatgcc gaccgagatg gcggcgacga agtcgcgcat ggggtgggcg
     6421 gtcaacttgc cggcggcgac gagaccggcg agggcgtccc aggcggccac gcaggcgccc
     6481 gtgatggagg cggtgcgggt gccgccatcc gcctgcagga cgtcacagtc aagggtgatc
     6541 tggcgttcgc cgagggcctg cagatccacc accgcacgca ggctgcggcc gatcaggcgc
     6601 tggatctcct gggtgcgccc gctctgcttg cccttggcgg cttcccgcgg gctgcgggta
     6661 tgggtggagc ggggcagcat gccgtattcg gcggtgagcc agccctggcc cttgccgcgc
     6721 aggaaggggg ggacggactc ttcgacactg gcggtgcaca gcacgcgggt gtcaccgaat
     6781 tcgaccagca ccgaaccttc cgcgtggcgg gtgaaatggc gggtgaggcg gacggagcgc
     6841 aactcgcccg gggcgcgccc gctcggacgg gcaaaggaag tgtcagtcat ttgaacttgt
     6901 ggatggcagc gttgatctcg cggatggcct tttcgatctc atcctcgctg agatcctcct
     6961 ggtccggacg ccgacggccg aagccttcca tctgggtggt gtaggcgtcg gccggcatgc
     7021 cgcgggtgga gacggtctcc gggctgtctt cctgcagttc gtcgtgccag gcgacggcga
     7081 tcagggacat gttgtcgcac tgggcgccgc cggcagcttc ggagaggtcc atcagggccg
     7141 gcacgccttc catgacgcgc ccgttggaca gcgtgcgcac cagggagtcg gtgtcgatgg
     7201 ggccccacac gccgtcggaa cacatggcga tgacgtcgcc gttggccagc gtgacggccc
     7261 gtgagaattc gatctggggt gggtgattgc cgcccaggca gctgaagatg cggttgcggc
     7321 cggggtgcga ggcggccgct t
//
LOCUS       DAOQPA010000145         7339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5683_length_7339_cov_17.186985, whole genome shotgun sequence.
ACCESSION   DAOQPA010000145 DAOQPA010000000
VERSION     DAOQPA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_7339_cov_17.186985"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="ispG"
                     /locus_tag="PKX01_15135"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1180)
                     /gene="ispG"
                     /locus_tag="PKX01_15135"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HMZ77517.1"
                     /translation="MSTDTMDLLLSGRAAARHPTRQVRVGRVVIGSDAPVVVQSMTNT
                     DTADDFATAMQVAQLARAGSELVRITVNNDEAAKAVPRIRERLLQMNLDVPLVGDFHY
                     NGHTLLTHHPACAEALAKFRINPGNVGQGAKRDTQFAAIVELACQYDKPVRIGVNWGS
                     LDQSVLARIMDENARRAVPRDAGAVMREALVTSALESATKAEEYGLAGDKIVLSAKVS
                     SVQDLIAVYRDLAARCDYPLHLGLTEAGMGSKGIVASTAAMAVLLQEGIGDTIRVSLT
                     PEPNGSRTQEVIVAQEMLQTMGLRAFTPMVTACPGCGRTTSTLFQELASDVQSFVRDN
                     MPVWREQYDGVENMTLAVMGCVVNGPGESKHASIGISLPGTGESPAAPVFEDGVKTLT
                     L"
     gene            complement(1177..2154)
                     /locus_tag="PKX01_15140"
     CDS             complement(1177..2154)
                     /locus_tag="PKX01_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HMZ77518.1"
                     /translation="MNDSVDNAVSLASSSVPGVTLRGERERQGLSIAEVAATLKLTPR
                     QIEAIEAGRFEDLPGFAFAKGFVRNYARLLHLDPQPLLAALEAPDDARLMELTPVSNA
                     KGDMPQVGRGRFRRSVLPGVVAALALLSVVVVGWYYDSLRRKPAEELMASLPQTPPAV
                     ATPVEPPVPAVPPAGESAAAGVPAPVPVDGAAAVAAPAVLPAGTAGTATPVPAVPTPA
                     VISAAPLTESRTAEAKAPPAVEARVGDGASERLDFDFEQDAWVEVKDGRGKTVLSKLG
                     QAGGRLGVEGQAPFTLVVGNARYVKLQRNGKPVDLAASTKVTVARLKLE"
     gene            complement(2151..2948)
                     /gene="pilW"
                     /locus_tag="PKX01_15145"
     CDS             complement(2151..2948)
                     /gene="pilW"
                     /locus_tag="PKX01_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HMZ77519.1"
                     /translation="MKRLLGVGALVALLGGCAVPAGPAPSGSYVAQRPQSQAPVTTDA
                     RRKARVHVELGQAYLEAGRYGVALDEAKMAAAYDPGYAPTFQLMGLVHMFLEENDVAA
                     ANFEHALRLVPGDPEIANSYGWFLCSMGKEKAGIEQLAGAARNPYYQTPTRAFTNMGL
                     CYLRLKDDGAAEQQFIRALEADNGNRQALFQLAAIQYRRANYEGAKRVIVDLHEAGEP
                     TPESLWLAIRIEHRLGDRTAEASLVQQLRRRFPASPEYQAFLQGQYQ"
     gene            complement(2945..4048)
                     /gene="rlmN"
                     /locus_tag="PKX01_15150"
     CDS             complement(2945..4048)
                     /gene="rlmN"
                     /locus_tag="PKX01_15150"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMZ77520.1"
                     /translation="MELPVVNLLDFDAAALTDWFVQQGEKPFRAKQVLRWVHRFGVTD
                     FEQMTDVAKSMRARLAETACITPPVPVRDSVSTDGTRKWLLDVGNANAVETVFIPETN
                     RGTLCISSQAGCALDCAFCSTGKQGFNRNLTAAEIIGQLWLANRLLGATGDGERVISN
                     VVMMGMGEPLANFDNVVSALRLMLDDNAYGLSRRRVTVSTSGIVPAMDRLRDACPTAL
                     AVSLHASNDALRDRLVPINQKYPLRELMAACQRYLVKAPRDFITFEYVMLDGVNDTDA
                     HARELVTLTRDVPCKFNLIPFNPFPQSGFDRSPAPRIRRFAEILIEAGIVTTTRKTRG
                     DDVDAACGQLAGQVQDKTKRTAARVVPIAEVRP"
     gene            complement(4094..4519)
                     /gene="ndk"
                     /locus_tag="PKX01_15155"
     CDS             complement(4094..4519)
                     /gene="ndk"
                     /locus_tag="PKX01_15155"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMZ77521.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEGAGLKIIAAKLAYLSE
                     REAGEFYAVHKERPFFKDLVSFMTSGPVMIQVLEGEGAIAKNRELMGATDPKKAAPGT
                     IRADFAESIDANAVHGSDAPETAAVEVAFFFPGMNVYSR"
     gene            complement(4730..6526)
                     /locus_tag="PKX01_15160"
     CDS             complement(4730..6526)
                     /locus_tag="PKX01_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ77522.1"
                     /translation="MSTYNAPIRDMQFVMKELAGLDEVAALPGCEEATPDLVDAILEE
                     AGKFAGGVLAPLNRIGDTQGPRWDNGVVHTSPGWKDAYTQFAEAGWTALACEPEFGGQ
                     GLPKLVSTAVMEMWKSANMAFSLCPMLTNGAIESIKLRGTDEQKAAYLPKMVSGEWTG
                     TMNLTEPQAGSDLAAVRTRAVPQGDGTYKIFGQKIFITYGEHDMTDNIIHLVLARLPD
                     APEGVKGISLFVVPKFLLNADGTPGTRNDVHCVSIEHKLGIHGSPTAVLAFGDNGGAI
                     GTLVGEANRGLEYMFIMMNEARFAVGMEGLALSERAYQQALGYAKDRIQGTDAGVRGG
                     PKVNILHHADVRRMLMVIKSTTEAMRALAYVVGAATDKAHYHADDAERARAQAFVDLL
                     IPVVKGWLTENSVELTSLGVQVHGGMGFIEETGAAQHFRDARITPIYEGTTAIQANDL
                     IGRKIAKEGGQTVKAVVAEMRQVEAQLAEQAGDEFAAIRKSLSAGITAVEEAVAYILA
                     TFGQDIKAASVGSVPFLKLLGIVTGGWQMGRAALVSARRLAEGSGDATFYKTKIVTAR
                     FYADHVLSQAPGLAYAVVNGAAGALALEEDQF"
     gene            complement(6585..7250)
                     /locus_tag="PKX01_15165"
     CDS             complement(6585..7250)
                     /locus_tag="PKX01_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HMZ77523.1"
                     /translation="MNLAADLFPLGWHVVAILGGLGVGLWVVRTGPWKRLLDGHQLNA
                     VLGATVILALLWSINAGVRPGLNLHLVGGMAVTLIFGPQLALLVLALTLGAITLNGSI
                     AWSAYPINLLLMVIIPVALATLAFRLVERVLPKHFFIYIFVNAFLCAALVVLAQGTLA
                     SASLFLAGAYEADVLLTEYLPFFLLLGFSEAWLSGMALTLLVIYRPEWVASFDDRSYL
                     MNK"
BASE COUNT         1287 a         2417 c         2432 g         1203 t
ORIGIN      
        1 gcagggtcag ggtcttgacc ccgtcctcga acaccggcgc cgccgggctc tcgccggtgc
       61 cggggagcga gatgccgatg ctggcgtgct tgctttcgcc ggggccattg acgacgcagc
      121 ccatcacggc gagggtcatg ttctccacac cgtcgtactg ctcacgccaa acgggcatgt
      181 tgtcacggac gaaggactgc acgtcggagg caagctcctg gaacagggtg ctggtggtac
      241 gaccgcaacc cggacaggcc gtcaccatcg gcgtgaaggc gcgcaggccc atggtctgga
      301 gcatctcctg ggcgacgatc acctcctgcg tgcggctgcc gttgggctcc ggcgtgagcg
      361 acacgcggat ggtgtcgccg atgccttcct ggagcagcac ggccatcgcc gcggtggacg
      421 ccacaatgcc cttggagccc atgccggcct cggtgagccc caggtgcaac ggatagtcgc
      481 agcgggccgc aagatcgcga tacacggcga tcagatcctg cacgctgctg accttggcgg
      541 acaacacgat cttgtcgccg gccagcccgt actcctcggc cttggtcgcg gactccaggg
      601 ccgacgtgac cagcgcctcg cgcatcacgg cccccgcatc ccgcggaacg gcgcggcgcg
      661 cgttctcgtc catgatgcgg gcgagcacgg actggtccag gctgccccag ttcacgccga
      721 tgcgcaccgg cttgtcgtac tggcacgcca gctcgacgat ggcggcgaac tgggtgtcgc
      781 gcttggcgcc ctgccccaca ttgccgggat tgatgcggaa tttggccagc gcttcagcgc
      841 aggcgggatg atgggtgagc agcgtgtggc cgttgtagtg gaaatcgccc accagcggca
      901 cgtccagatt catctgcagg agccgctcgc ggatccgcgg cacggccttg gcggcctcgt
      961 cgttgttgac cgtgatgcgc accagttcgg agcccgcccg cgccagctgg gcgacctgca
     1021 tggcggtggc gaaatcgtcc gcggtatcgg tgttggtcat ggactgcacc accaccggcg
     1081 cgtcggatcc gatgacgaca cgcccaaccc ggacctgccg ggtcggatgg cgggccgccg
     1141 cgcggccgga aagaagaaga tccatcgtgt ccgtactcat tccagcttca ggcgggccac
     1201 cgtgaccttg gtggacgccg ccaggtcgac gggcttgccg ttgcgttgca gtttgacgta
     1261 gcgcgcattg cccaccacca gtgtaaaggg cgcctggccc tccacaccga gtcggccgcc
     1321 cgcctgtccg agcttggaca ggaccgtctt gccacgtccg tccttcacct caacccaggc
     1381 atcctgttca aaatcgaaat ccagacgctc cgacgcaccg tcgcccaccc tggcctccac
     1441 cgctggaggt gccttcgcct cggcggtccg actttccgtg agaggcgccg cggagatcac
     1501 cgcaggcgtc gggaccgctg gaaccggagt cgcagtgccg gccgtgccgg cgggcaggac
     1561 cgcgggcgca gcaaccgccg cagcgccgtc caccgggacc ggcgcgggaa cacctgccgc
     1621 cgcggactcg ccggcgggcg gcacggccgg gaccggcggt tcgaccggcg tggcaacagc
     1681 cggcggtgtc tgcggcagac tcgccatcag ttcctccgcg ggcttgcgcc gcagggaatc
     1741 gtagtaccag cccaccacca ccacgctgag caaggccaag gccgcaacga caccgggcag
     1801 caccgaacgc cggaaccgcc cccgcccgac ctggggcatg tcgcccttgg cattggagac
     1861 cggcgtcagt tccatcagcc gggcatcgtc gggtgcctcg agggccgcca gcaggggctg
     1921 cggatcaaga tgcagcaggc gcgcatagtt gcgcacgaag ccctttgcga aggcaaaccc
     1981 cggcagatcc tcaaagcggc ccgcctcgat ggcctcgatc tgccgcggcg tcagcttgag
     2041 tgtcgccgcc acctcggcaa tcgacagccc ctgacgctcc cgttcgcccc gcagggtgac
     2101 gccgggcacg gaggaagacg ccagggacac ggcgttatcg acggaatcgt tcactgatac
     2161 tgcccttgca agaaagcctg atattcgggt gacgcgggga aacggcgccg cagttgctgg
     2221 acgagggatg cctcggccgt gcggtcgccc agtcgatgtt cgatgcggat ggccagccac
     2281 aggctctcgg gtgtcggctc gcctgcttca tgcagatcga cgatgacgcg cttggcgccc
     2341 tcgtagttgg cccgccggta ctggatcgcc gcgagctgga acaaggcctg gcggttcccg
     2401 ttgtcggctt ccagcgcgcg gatgaactgc tgctccgccg cgccatcgtc cttgagacgc
     2461 agatagcaga gccccatgtt ggtgaaggcg cgggtcggcg tctggtaata cggattgcgg
     2521 gccgcgcccg ccagttgctc gatcccggcc ttttccttgc ccatggaaca caggaaccag
     2581 ccgtagctgt tggcgatttc cgggtccccg ggcacaaggc gcagcgcgtg ctcgaaattg
     2641 gccgcggcga cgtcgttctc ttcgaggaac atatgcacca gccccatgag ctggaaggtc
     2701 ggcgcgtagc ctggatcata agcggccgcc atcttggctt cgtcaagcgc gacgccgtag
     2761 cggccggcct cgagataggc ctgaccgagt tccacatgga cgcgcgcctt gcgccgggca
     2821 tcggtggtga ccggtgcctg ggactgggga cgctgggcca cgtaactgcc cgacggcgcc
     2881 ggtccggccg ggacggcgca ccctcccagc agcgcgacca gcgcaccgac gccgagcagc
     2941 cgcttcacgg gcgcacctcg gcgatcggca cgacccgtgc ggcggtccgc ttcgtcttgt
     3001 cctgcacctg accggcgagc tggccacagg ctgcgtctac gtcgtcaccc cgggtcttgc
     3061 gggtggtggt gacgatgccg gcctcgatga ggatctcggc aaaacgccgg atacgcggcg
     3121 ccggcgaacg gtcgaagccg gattgcggga acggattgaa cggaatcagg ttgaacttgc
     3181 agggaacatc ccgcgtcagg gtgacgagtt cgcgggcgtg ggcgtcggtg tcgttcaccc
     3241 cgtccagcat cacgtattca aaggtgatga agtcccgcgg cgccttgacc agatagcgct
     3301 gacaggcggc catcaattca cgcagggggt atttctgatt gatcggcacg aggcggtcgc
     3361 gcaaggcgtc gttggacgcg tgcagggaca cggccagggc cgtgggacag gcgtcgcgca
     3421 ggcggtccat ggccggcacg atgccggagg tggacacggt gacgcgccgc cgggaaagtc
     3481 cgtaggcgtt gtcgtcgagc atcaggcgca gggccgagac gacattgtcg aaattggcaa
     3541 ggggctcgcc catgcccatc atcaccacat tgctgatgac ccgctcgccg tcgccggtgg
     3601 caccgagcag acggttggcg agccacagct ggccgatgat ttccgccgca gtgaggttac
     3661 ggttgaagcc ctgcttgccc gtcgagcaga aggcgcagtc gagggcgcag ccggcctgcg
     3721 acgagatgca cagggtgccg cgattggttt cggggatgaa caccgtctcc accgcgttgg
     3781 cattgcccac gtcaagcagc cacttgcggg tgccgtccgt ggagacgctg tcgcgcaccg
     3841 gcaccggtgg ggtgatgcag gctgtctccg cgagcctggc ccgcatggac ttggccacgt
     3901 cggtcatctg ctcgaagtcg gttacgccga agcgatgcac ccagcggagc acctgcttgg
     3961 cgcgaaaagg cttctcaccc tgctggacga accagtcggt gagggcagcg gcatcgaaat
     4021 cgagaagatt gaccacgggc agttccattc agtccggcag gcggcgcaac gcgcacccgc
     4081 cggaaagaga tcctcagcgg ctgtaaacgt tcatgccggg gaagaagaaa gccacttcca
     4141 cagccgcggt ttcgggcgcg tcggagccat gcacggcgtt ggcatcgatg gactcggcga
     4201 agtcggcgcg gatggtgccc ggcgcagcct tcttcgggtc ggtggcaccc atcaattcac
     4261 ggttcttggc gatggcgcct tcaccttcca gcacctggat catgacgggg ccggaggtca
     4321 tgaaggacac cagatccttg aagaagggac gctccttgtg cacggcgtag aactcgccgg
     4381 cttcacgctc ggacaggtag gccagcttgg cggcaatgat cttcaggccg gcgccttcaa
     4441 agcgggcgta gatctggccg atcacgttct tggcgacggc gtcgggcttg atgatggaca
     4501 gggtgcgttc gatggccatg gtacggggct ccagatgagt cgaagaagtc ggtaagggtt
     4561 aaaaaaccga aagcttggaa ttctagcagg tacaggtgca gattcgacca tttttcggtc
     4621 acgcccaacc gaatgcacac gtgatccatg aaaaacgggg gccgaagccc ccgcacctgc
     4681 gtcctgaagc ccgcttgcca acgcgccaga acggcgcgcc ggcagccact cagaactggt
     4741 cctcttccag cgccagcgcg ccggccgcgc cattcaccac cgcgtaagcc agaccaggcg
     4801 cctgggacag cacgtggtcg gcgtagaagc gggccgtgac gatcttggtc ttgtagaagg
     4861 tggcatcacc gctgccttcc gcgaggcgcc gggccgacac gagggccgcg cgccccatct
     4921 gccagccgcc ggtgacgatg cccagaagct tgaggaaggg cacggaaccg acggaggcgg
     4981 ccttgatgtc ctgaccgaag gtggcgagga tataggcgac cgcttcttcc accgcggtga
     5041 tgccggcgga cagggacttg cggatggccg cgaactcatc gccggcctgc tcggcgagct
     5101 gggcttcgac ctgacgcatc tcggccacca ccgccttgac cgtctgtccg ccttccttgg
     5161 cgatcttgcg gccgatcagg tcgttggcct ggatggccgt ggtgccttca tagatcggcg
     5221 tgatgcgggc gtcgcggaag tgctgggccg cgccggtctc ctcgatgaag cccatgccgc
     5281 catgcacctg cacacccaac gaggtgagct cgacagagtt ttcggtcagc cagcccttga
     5341 ccaccggaat cagcagatcg acgaaggcct gggcgcgcgc ccgctcggcg tcatccgcgt
     5401 ggtaatgggc cttgtcggtg gccgccccga ccacgtaggc cagcgcccgc atcgcctcgg
     5461 tggtggattt gatgaccatc agcatgcgcc gcacatccgc gtggtgcagg atgttgacct
     5521 tggggccccc gcgcacgccg gcgtcggtgc cctggatacg gtccttggcg tagcccagcg
     5581 cctgctggta ggcccgctcg gacagggcca gaccttccat tcccaccgcg aagcgcgcct
     5641 cgttcatcat gatgaacatg tactcgagac cacggttggc ctcacccacc agggtcccga
     5701 tggcaccgcc gttgtcgccg aaggccagca ccgccgtggg gctgccatgg atgcccagct
     5761 tgtgctcgat ggacacgcag tgcacatcgt tacgggtgcc gggcgttcca tcggcgttga
     5821 gcaggaactt gggcaccacg aacagggaaa tgcccttcac cccttccggc gcgtccggca
     5881 ggcgggccag caccaggtgg atgatgttgt cggtcatgtc gtgctcgccg taggtgatga
     5941 agatcttctg cccgaagatc ttgtaagtac catcaccctg gggcacggcg cgggtacgca
     6001 cggcggccag atcggaaccg gcctggggct cggtcaggtt catggtgccg gtccattcgc
     6061 cgctcaccat cttgggcagg taggccgcct tctgctcgtc cgtaccgcgc agcttgatgg
     6121 actcgatggc gccattggtc agcatcgggc acagggagaa ggccatgttg gccgatttcc
     6181 acatttccat caccgccgtg gacaccagct tgggcagacc ttgtccgccg aattccggct
     6241 cgcaggccag ggccgtccag ccggcttcgg caaactgcgt gtaggcgtcc ttccagcccg
     6301 gcgaggtatg caccacgccg ttgtcccagc gcgggccctg ggtatcacca atgcggttga
     6361 ggggcgccag cacgccgccg gcaaacttgc ccgcttcctc caggatcgca tccaccagat
     6421 cgggggtggc ttcctcacac cccggcagtg cggccacttc atccagaccg gccagctcct
     6481 tcatgacgaa ctgcatgtcg cggatcggtg cgttgtaggt gctcatgtgt ctttctcctc
     6541 gaaatcttgt ttgttttgag tggatcggcc cgggctgaaa gggcctattt gttcatcaga
     6601 taactgcgat catcgaaact cgccacccac tccgggcgat agatcaccag gagggtcagc
     6661 gccatccccg acagccacgc ctcggaaaag cccagcagca ggaagaaggg aaggtattcc
     6721 gtcaacagca catccgcctc gtaggccccg gccaggaaca gggatgcgga ggctagggtg
     6781 ccctgcgcca gcaccacgag ggccgcacag agaaaggcgt tgacgaagat gtaaatgaag
     6841 aaatgcttcg gcagcacgcg ctcgaccagg cgaaacgcca gcgtcgcgag cgcgaccggg
     6901 atgatcacca tcagcagcag attgatcgga tacgccgacc aggcaatgct gccgttcagg
     6961 gtgatggcac ccagcgtcag cgccaggacc agaagcgcca gttgcggacc gaagatcagg
     7021 gtcaccgcca tcccgcccac gagatggaga ttgagccccg gcctgacacc cgcattgatg
     7081 ctccacagca gcgccaggat caccgtggcg cccagcaccg cattgagctg atgcccatcc
     7141 agcaggcgct tccagggccc ggtacgcacg acccacagcc cgacccccaa tccacccagg
     7201 atggcaacga cgtgccatcc aagcggaaag aggtcggctg caagattcat gacgcccgcg
     7261 gggcccggat cagagggacg aggtcagctg gggcaccgct tcgaacagat cggccacgag
     7321 gccgtagtcg gccacctgg
//
LOCUS       DAOQPA010000146         7324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5696_length_7324_cov_20.555922, whole genome shotgun sequence.
ACCESSION   DAOQPA010000146 DAOQPA010000000
VERSION     DAOQPA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_7324_cov_20.555922"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="asd"
                     /locus_tag="PKX01_15170"
     CDS             <1..1125
                     /gene="asd"
                     /locus_tag="PKX01_15170"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMZ77524.1"
                     /translation="MKRVGLVGWRGMVGSVLMQRMREENDFALIEPVFFTTSNPGGKA
                     PNFGKDAPALKDARSIDELKAMDIVITCQGGDYTNEVYPQLRAAGWNGHWIDAASALR
                     MDPKAVIILDPVNLNVIQDSLAKGGKDWIGGNCTVSLMLMALGGLFQRDLVEWATSMT
                     YQAASGAGAQNMRELLTQMGELNRSVKELLDDPASAILDIDREVAGTMRDDAFPSSNF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKGGVETNKILGRGEGFGSPAVPIDGLCVRIGAM
                     RCHSQALTLKLKKDIPLDEIEGLLASANDWVKVVPNQREASMRDLTPTAVTGTLTVPV
                     GRLRKLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRVLLEA"
     gene            1262..4222
                     /locus_tag="PKX01_15175"
     CDS             1262..4222
                     /locus_tag="PKX01_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HMZ77525.1"
                     /translation="MHKKTLNNRLRLSLMALAIGTVSLGSGAAGLGRITVMSSLGQPL
                     RAEVEIAATPEELQNITARLASADAFRQAGIDYSPSVTGLRMAVERRGNGAVVRVSSD
                     RPINEPFVDMLVELNWSAGRLIREYTFLLDPPDAGAPRAAAVAVPEVRSDMVRPGVAP
                     APAAVRRAPASRPAAVPTASDRSYLVKPGDTLNRIAMETRPDGVSVDQMLSALFQGNR
                     GAFAADDINRLQAGKVLRIPDADGVRQLAPGGRQVVAQSSDFGRYRQRLAEQAAASAP
                     KEAPARQSASGRIGARVEDKAQGLPAGSDQLKVSKGADAASLAGGGADKPSSRLQALE
                     EDLVSRDKALKDANARLAELEKSIRELQRLVELKSQGLAQTQDQAAKPAAAPAVVPPP
                     PPAPVPPPAAVVPPPEAAIKPPEAEKPADLAPPPPPPPAPEVKPAPPPPPAPAPAPAE
                     ASSGPDMSLLLGGGGIAALLLGWGAYRLFGKRRNNGAPTTTPAYSEFGEGSSSVFGSA
                     GGQSVDTGSSSQIQTDFSQSGLSSIDTDEGVDPVAEADVYMAYGRDAQAEEILLDALK
                     TDPGRTGVHLKLLEIYAQRKNLKQFETTATELYSLTGGNGPDWEKAAALGRKVDPDSP
                     LYRIPVPGAMPGPSAIPVPAVSELGPHTIAGTMAPATGFVQAGEARQAPPMSARPAPA
                     PAPIAPSGDDDPLESPSQLKDTWALPGELNQYTSLNDLDHHAPSAPEPVAPSRPASLD
                     FDLNLEASSPSGNLATIVTGDGNGPASAGGLDFDLGFEQSVTRGAGATMVVGDEFGRT
                     MEVTSTRPQRVGEDFGLPDPSDLDFEISSEFGERNANPPVAPSVDVDLTATLADEGIR
                     PPNDSATIDLERTNFDGNLLDFDFDLDDRSKADVHPGQATQTDMPAVDLSGIDLDLDR
                     PTVEVDLPDAGPPTEPPLDMDLHQEVATKLELARAYEEMGDKDGARELVEEVLREGNG
                     RQKDEAQAILARLG"
     gene            4249..4848
                     /locus_tag="PKX01_15180"
     CDS             4249..4848
                     /locus_tag="PKX01_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HMZ77526.1"
                     /translation="MLHPATRLLLWSAWVVVVQIARWPFVGVIGLFVLVAGFWAARTR
                     CARLLRRVRILLGVLVILFAFFTPGEALLPGLGAAGPTREGLAAAGLHGLRLVSVVVL
                     VALLLEKTGQAALVGGLTSLARPLHVLGLSADRLAVRMLLVLQYASAPPVRGWRAFLV
                     EDERPAEPGVIVLPKVRPGWPDVFVWSVLAIGVAAWMFA"
     gene            4850..5653
                     /gene="truA"
                     /locus_tag="PKX01_15185"
     CDS             4850..5653
                     /gene="truA"
                     /locus_tag="PKX01_15185"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMZ77527.1"
                     /translation="MPPIRIALGVEYAGDAFEGWQTQPHGRTVQDVLEAAVCVIAQAP
                     IHLVCAGRTDTGVHATTQVAHFDTAAVRPPEAWVRGVNSHLPRGVSVLWSQTVAADFH
                     ARFSAVSRHYRYLLLNRRVRPALLAGRVGWLHDGLDVEAMTAAADCLVGEHDFTSFRA
                     AECQAKSPVRTVYRVAVGRVGDLVVFDFHANAFLHHMIRNLVGALVMVGRGRKPVAWM
                     ADVLAARNRSVAAPTFAPDGLYLCGVEYPPRWPLPGGGRIIVPPQLPLG"
     gene            5658..6281
                     /locus_tag="PKX01_15190"
     CDS             5658..6281
                     /locus_tag="PKX01_15190"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HMZ77528.1"
                     /translation="MHRTRIKICGLTREEDVDAAVGAGADAIGFVFYPPSPRHVSFAR
                     AAELARRVPPFVTVVGLFVNTPRAEVDEALRQVPIQLLQFHGDEEEADCAGFGRPYVK
                     AARMRPGLDLLKYAARFPGAQGLLLDAFVEAYGGGGETFDWSLIPSGLPLPLILSGGL
                     HAGNVGEAIRRVRPWAVDVSSGVEAAKGIKDAARITEFVAGVRHAHG"
     gene            6268..>7324
                     /gene="trpB"
                     /locus_tag="PKX01_15195"
     CDS             6268..>7324
                     /gene="trpB"
                     /locus_tag="PKX01_15195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMZ77529.1"
                     /translation="MHMADAPYDLPDARGHFGPYGGVFVSETLIPALAELREAYAAAQ
                     QDPEFIAEFEYELKHYVGRPSPIYHAKRWSGLLGGAQIYLKREDLNHTGAHKVNNCIG
                     QALLARRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDVRRQAANVYRMKL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVGNTFYIIGTVAGPHPYPMMVRDFQAVIG
                     QECIEQMPPLAGRQPDAVIACVGGGSNAMGIFHPYIGFPDVRLVGVEAAGHGLDSGQH
                     AASLSAGRPGVLHGNRTYLLQNEDGQIIETHSISAGLDYPGVGPEHAWLKDSSRAEYV
                     GITDAEALQAFHDLCRFE"
BASE COUNT         1179 a         2347 c         2492 g         1306 t
ORIGIN      
        1 atgaaaagag tgggtctggt aggctggcgc ggcatggtgg gttccgtcct gatgcagcgc
       61 atgcgtgagg aaaacgattt cgcactgatc gagccggtgt tctttaccac ctccaatccg
      121 ggcggcaagg cgcccaactt cggcaaggac gcgccggcgc tcaaggacgc ccgcagcatc
      181 gacgagctga aggcgatgga catcgtcatc acctgccagg gcggcgacta caccaacgag
      241 gtgtaccccc agttgcgcgc cgctggctgg aatggccact ggatcgacgc tgcctccgcg
      301 ctgcgcatgg acccgaaggc cgtgatcatc ctcgatccgg tcaacctcaa cgtcatccag
      361 gactccctgg ccaagggcgg caaggactgg atcggcggca actgcaccgt gtccctgatg
      421 ctgatggcgc tgggcggcct gttccagcgt gacctggtcg aatgggccac ttccatgacc
      481 taccaggccg cctccggtgc cggcgcgcag aacatgcgcg agctgctcac ccagatgggc
      541 gagctcaacc gctccgtgaa ggaactcctc gacgatccgg cctcggcgat cctcgacatc
      601 gaccgggaag tggccggcac catgcgggac gacgccttcc cctcgtccaa cttcggcgtc
      661 cccctggccg gctccctgat tccctggatc gacaaggatc tgggtaacgg ccagtccaag
      721 gaagaatgga agggcggtgt cgagaccaac aagatcctcg gccgcggtga aggcttcggc
      781 agcccggcgg tgcccatcga cggcctttgc gtgcgcattg gcgccatgcg ctgccacagc
      841 caggccctga ccctcaagct caagaaggac atccccctcg acgagatcga aggcctgctc
      901 gcctcggcca acgactgggt caaggtggtg cccaatcagc gcgaagcctc catgcgtgac
      961 ctgacgccga ctgccgtgac cggcaccctg accgttccgg tcggccgcct gcgcaagctg
     1021 gcgatggggg gcgaatatct ttcggccttc acggtggggg accaactgct ctggggcgcg
     1081 gccgaaccgc tgcgccgcat gttgcgtgtg ctgctcgaag cgtgatggca atgaaggcgg
     1141 cgcaagccgc cttttttcat gcaatgtcct gtatggaaag gcttggcaaa agacttgaac
     1201 ttgcctcgcc aaacccatga tgttatgttc atcggcaaga cgatttcggt gagaagccgt
     1261 agtgcataag aaaaccctca acaatcggct ccggctgagt ctcatggcgc ttgcgatcgg
     1321 taccgtatcc ctcggcagcg gggcagcggg actgggtcgc atcaccgtca tgagcagcct
     1381 gggtcagccc cttcgcgccg aagtggagat cgcggcgacg ccggaggagt tgcagaacat
     1441 caccgcccga ctggcttccg cggatgcctt ccgtcaggcc gggattgact actcgccttc
     1501 cgtgaccggg ttacgcatgg ccgtcgagcg ccgtggaaat ggcgcggtgg tgcgggtgag
     1561 cagtgatcgg cccatcaacg agccatttgt agacatgctc gttgaactca actggtcggc
     1621 cgggcgcctg atccgcgaat acaccttcct gctcgatccg cctgatgcgg gggcgcccag
     1681 ggccgctgct gtcgccgtgc ctgaagtccg ttccgacatg gtccgtcccg gcgtggcgcc
     1741 tgccccggcg gcggtgcgtc gtgcacccgc gtcgcgtcct gccgcggtgc cgacggcgtc
     1801 ggatcgcagc tatctcgtca agccgggcga cacgctgaac cgtatcgcca tggaaacgcg
     1861 tcccgacggc gtgtcggttg atcagatgct gtcggcgctg ttccagggca accggggtgc
     1921 ttttgcggcg gacgacatca atcgtcttca ggccggcaag gttctgcgca ttccggacgc
     1981 cgacggtgtc cgccagctgg cacccggcgg gcgccaggtc gtggcccagt cgtccgactt
     2041 cggccgctat cgtcagcgtc tggctgagca ggcggctgca tctgcaccga aggaggctcc
     2101 ggcccgtcag agcgccagcg gtcgcatcgg tgcccgtgtc gaggacaagg ctcaggggtt
     2161 gccggccggc tccgaccagc tgaaggtttc caagggtgcc gatgccgctt cacttgctgg
     2221 cggcggtgcg gacaaacctt cgtcgcgtct tcaggcgctt gaagaagatc tggtgtcacg
     2281 ggacaaggcg ctgaaagatg ccaatgcccg gctggccgaa cttgaaaaaa gtattcgtga
     2341 actgcaacgg ctcgtcgaac tgaagagcca agggctcgcc cagactcagg atcaggcggc
     2401 caagccggct gcagcgcctg ccgtggtgcc tcctccgccg ccagcgccgg tgccaccgcc
     2461 tgccgcagtg gttccgccgc cggaagcggc catcaaacct cctgaggccg agaagccggc
     2521 ggatctggcg cctccgccgc cgcccccgcc tgcaccggag gtcaagcccg cgccgccccc
     2581 gccgcctgca cccgctccgg cgccggccga ggcgtcttcg gggcctgaca tgtcgctgct
     2641 gctggggggt ggaggaatcg ccgcccttct gctgggctgg ggggcttatc gtctgtttgg
     2701 caaacggcgc aacaacggcg cgccgacgac gacgcccgcc tattcggagt ttggcgaagg
     2761 atccagctcc gtctttggaa gtgccggtgg acaaagtgtc gacacgggca gcagcagcca
     2821 gatccagacc gatttcagtc aatcgggtct gtcgtccatc gatacggatg aaggcgttga
     2881 cccggtggct gaggcggacg tctacatggc ctacgggcgc gatgcccaag ccgaggagat
     2941 ccttctcgac gcgctcaaga ccgatccagg gcggacgggc gtgcatctca agttgttgga
     3001 gatctatgcc cagcgcaaga atctgaagca attcgagacc acggccaccg agctttattc
     3061 cctcacaggc ggcaacggcc ccgattggga aaaggcggct gcgctggggc ggaaggtgga
     3121 tccggacagt ccgctctacc ggattccggt tccgggtgcg atgcctggcc cgtcggccat
     3181 tccggtgcct gccgtgtcgg aactcggtcc ccacacgatt gcggggacga tggcaccggc
     3241 caccggcttc gttcaggctg gcgaagcgcg tcaggcgccg ccaatgtcgg ctcgcccagc
     3301 gcctgcgccg gcccccatcg cgccctccgg ggacgacgat ccgttggagt cgccgtccca
     3361 actcaaggac acctgggcct tgccggggga actcaaccag tacacctcgc tcaacgatct
     3421 cgaccatcac gcgcccagtg cgcccgagcc cgttgcgccg tccaggcccg catcgctcga
     3481 tttcgatctg aacctggagg cgtccagtcc ctccggaaat ctggcaacga tcgtgacggg
     3541 cgacgggaat ggtccggcgt cggcaggcgg cctcgatttc gatctcggat ttgaacagtc
     3601 cgtcacgcgc ggggccggtg ccaccatggt ggtcggcgat gagttcggtc gcaccatgga
     3661 agtgacgtcc acccgtcccc agcgggtcgg cgaagatttt ggcttgccgg atcccagcga
     3721 tctggatttc gagatcagtt ccgaattcgg tgagcggaac gcgaatccgc cggtcgcgcc
     3781 gtcggtcgac gtggatctca cggccacgct cgccgacgag ggtattcgtc cgccaaatga
     3841 ttcggccacc atcgatctgg agcgcacgaa cttcgacggc aacctgcttg atttcgattt
     3901 cgacctggat gatcgctcaa aggccgatgt gcatccgggg caggcgactc agactgacat
     3961 gcccgcggtg gatttgagcg ggatcgatct ggatctggac aggccgacgg ttgaggtcga
     4021 tttgccggat gcgggtccgc cgaccgaacc gccgctcgac atggatctgc atcaggaggt
     4081 cgcgaccaag ctcgaactgg cgcgtgccta cgaagagatg ggtgacaagg acggtgctcg
     4141 cgagctggtc gaagaagtgc tgcgtgaggg caatggtcgg cagaaggacg aagcgcaggc
     4201 gattctcgcc cgcctgggct gattgctccc gtcgtcggct tcaagaggat gttgcatccg
     4261 gccactcgcc tgctcctgtg gtctgcgtgg gtggtagtgg ttcagatagc ccgatggccc
     4321 tttgtagggg tcatcgggct gtttgttttg gtcgcgggat tctgggcggc ccggacgcga
     4381 tgtgcccgct tgcttcgtcg ggtgcgcatc ctgctcggtg tgctggtgat cctgttcgct
     4441 tttttcacgc cgggtgaggc actgctgccc ggactggggg ccgccggccc gacacgggaa
     4501 ggtttggcgg ccgccgggct gcatggtctg cgcctggtga gcgtggtggt cctggtggcg
     4561 ctgctgctgg agaagaccgg gcaggcggcg ctggtagggg ggctgacgag tctggcgcgc
     4621 cccctgcatg tgctgggact gtcggcggat cggctggcgg tgcgcatgtt gctggtgttg
     4681 cagtacgcca gcgcgccgcc cgttcgtggc tggcgcgcct ttctggtcga ggacgagcgg
     4741 cctgccgagc ctggggtcat cgtactgccc aaggtgaggc ccggttggcc ggatgtattc
     4801 gtctggagcg tcctggccat tggcgtcgcg gcatggatgt ttgcctgata tgccaccgat
     4861 ccgaatcgct ttgggtgtcg aatatgccgg tgatgccttc gaaggctggc agacccagcc
     4921 ccatggacgc acggtccagg acgtgctcga agcggccgta tgtgtgattg cccaggcgcc
     4981 gatccatctg gtgtgcgccg ggcgaaccga cacgggcgtg catgccacca cgcaggtggc
     5041 gcatttcgac accgccgcgg tccgcccgcc ggaagcgtgg gtgcggggcg tcaacagcca
     5101 tctgccgcgc ggtgtgagcg tgctgtggtc gcagacagtg gccgccgatt ttcacgcgcg
     5161 cttcagcgca gtgtcgcgcc actaccgcta tctgttgctc aatcgacggg tccgtccggc
     5221 gctgctggca ggtcgggtgg ggtggttgca cgatggactc gacgtggagg ccatgacggc
     5281 agcggccgac tgcctggtgg gtgagcacga tttcacctcc ttccgggccg cggagtgcca
     5341 ggcgaagtcg ccggtgcgca cggtgtatcg ggtggcggtg ggtcgggtag gcgatctggt
     5401 ggtgttcgat ttccatgcca atgccttcct tcatcacatg atccgcaatc tggtcggagc
     5461 gctggtgatg gtcggcaggg gacgcaagcc ggtggcgtgg atggcggacg tgctggccgc
     5521 gcgcaaccgc agcgtggcgg cacccacctt cgcgccggac ggcctgtacc tgtgtggggt
     5581 cgagtatccg ccacggtggc cgctgcccgg tggcgggcgt atcatcgtgc ccccgcaact
     5641 tccgctgggg tgaccccgtg catcgaaccc gaatcaagat ctgcggcctg acccgcgaag
     5701 aagacgtgga cgccgccgtc ggcgccgggg ccgacgccat cggtttcgtg ttctatccgc
     5761 ccagtccgcg ccatgtgagc ttcgcgcggg cggccgaact ggcccggcgc gtgccgcctt
     5821 tcgtgaccgt cgtcggcctg ttcgtcaaca ccccgcgcgc cgaggtggac gaggccctgc
     5881 gccaggtgcc gatccaactc ctgcagttcc atggcgacga ggaggaggcg gactgtgccg
     5941 gcttcggtcg accctatgtc aaggcggcga ggatgcggcc ggggctcgat ctgttaaaat
     6001 acgcggctcg ctttccgggc gcccaggggc tgttgctgga cgcgtttgtg gaagcttatg
     6061 gtgggggagg cgaaaccttc gactggtccc tcatcccgtc cggcctgccg ctgcccctga
     6121 tcctgtcggg ggggctgcat gccggcaacg tgggtgaagc gatccggcgc gtgcggccgt
     6181 gggccgtgga tgtctccagc ggcgtggaag ccgccaaagg catcaaggac gccgcccgga
     6241 tcaccgaatt tgtagccgga gtccgacatg cacatggctg atgcccctta cgaccttccc
     6301 gacgcccgcg gccatttcgg cccctacggc ggcgttttcg tgtccgagac gctgatcccg
     6361 gcgctggccg aactgcgcga agcctatgcc gccgcgcaac aggatccaga gttcattgcc
     6421 gaattcgagt acgaactcaa gcattacgtg gggcgtccca gcccgatcta ccacgccaag
     6481 cgctggtccg gcctgctggg gggcgcgcag atctacctga agcgggaaga tctcaaccac
     6541 accggcgccc acaaggtgaa caattgtatc ggccaggcgc tgctcgcccg gcgcatgggc
     6601 aagccccgcg tgatcgccga gaccggtgcc ggtcagcatg gcgtggccac cgccaccgtg
     6661 gcggcccgct atggcatgga gtgtgtggtg tacatgggct ccgaagacgt gcgccggcag
     6721 gcagccaacg tctatcgcat gaagctgctg ggcgcgacgg tggtgccggt ggagagcggc
     6781 tccaagaccc tcaaggatgc cctcaacgag gccatgcgcg actgggtgac caacgtcggc
     6841 aacaccttct acatcatcgg cacggtggcg gggccgcatc cctacccgat gatggtgcgg
     6901 gacttccagg ccgtcatcgg tcaggaatgc atcgagcaga tgccgcctct ggcggggcgt
     6961 cagccggacg cggtgatcgc ctgcgtcggc ggtggatcca acgcgatggg catcttccat
     7021 ccctacatcg gctttcccga cgtgcgtctg gtgggcgtcg aagcggccgg gcacggcctc
     7081 gacagcggcc agcatgcggc ctccctgtcg gccgggcggc cgggggtgct gcacggcaac
     7141 cgcacctacc tgctccagaa tgaggacggc cagatcatcg agacccactc catctcggcg
     7201 ggtctcgact accccggggt cgggcccgaa cacgcctggc tcaaggacag cagccgcgcc
     7261 gaatacgtgg gcatcaccga tgccgaggcg ctgcaggcct tccacgacct gtgccgtttc
     7321 gagg
//
LOCUS       DAOQPA010000147         7263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_5775_length_7263_cov_18.570200, whole genome shotgun sequence.
ACCESSION   DAOQPA010000147 DAOQPA010000000
VERSION     DAOQPA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5775_length_7263_cov_18.570200"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..590)
                     /locus_tag="PKX01_15200"
     CDS             complement(60..590)
                     /locus_tag="PKX01_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HMZ77530.1"
                     /translation="MSPGQQHLERFIFWSRWFQAPLYLGLVAAQGVYVYLFMAELAHL
                     IGSVFESGGHISETETMLVVLGLIDVVMIANLLIMVIIGGYETFVSRLDLDNHPDQPE
                     WLSHVNAGVLKIKLSTAIIGISSIHLLKTFINAGNLPEHTIKWQVIIHVVFLLSALAM
                     ALVDKLMNQTVLLSKH"
     gene            694..2061
                     /gene="purB"
                     /locus_tag="PKX01_15205"
     CDS             694..2061
                     /gene="purB"
                     /locus_tag="PKX01_15205"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMZ77531.1"
                     /translation="MNLHPITALSPLDGRYESKVAALREHFSEFGLIRNRVKVEIEWL
                     KALAAAPELAEIAPFSAATVAELDGVIAHFSVTDGEAVKAIEATTNHDVKAMEYWLKQ
                     RLGHNPEVTRVTEFIHFGCTSEDINNVSHALMLRDGRDGVLLPALDRIIARLVELAHN
                     LADLPMLSRTHGQPASPTTLGKEMANVVHRLRRVRASIAGVELTAKFNGAVGNYNAHL
                     SAYPDFDWEGFNGRFIESLGLTFNPYTIQIEPHDAMAELYDATARANTIVLDLCRDIW
                     MYISLGYFKQKLKAGEIGSSTMPHKVNPIDFENAEGNLGLANGVLRHLADKLPVSRMQ
                     RDLTDSTVLRNMGVAFGYAVLAYDSCLRGLGKLEADPARLAADLDACWEVLAEPIQTV
                     MRRYGVPNPYEQLKELTRGKGITRDALQAFIATLAIPEAEKTRLLAMTPASYIGKAAE
                     LARRI"
     gene            2119..2436
                     /locus_tag="PKX01_15210"
     CDS             2119..2436
                     /locus_tag="PKX01_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HMZ77532.1"
                     /translation="MAFADKLKTLPGVSHLAALHLLDGAGQVVGTIENKPGQAGSLAV
                     YNHLAQLHGAITPDAATQGIELYAEHSEDARLNPGKHPNIDRLIGLVERGETLRVKHV
                     FAA"
     gene            complement(2497..2865)
                     /locus_tag="PKX01_15215"
     CDS             complement(2497..2865)
                     /locus_tag="PKX01_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77533.1"
                     /translation="MPLRFPPPVVRTAFALALVVIFWLAMIPLPEALTVFSFQDKVEH
                     TGAFVLLTLMGVTGFPLRRTAVAAGLVAYGLLIEVCQHRFTTNRVGDPWDWLADSVGV
                     FIGLRLAGWFARRVVNIARA"
     gene            3275..3685
                     /locus_tag="PKX01_15220"
     CDS             3275..3685
                     /locus_tag="PKX01_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77534.1"
                     /translation="MSRIHSRRIVPSSKRAQAAWVLYGALLGNLHDAGEAIERCAADR
                     GRDALFQSQALWESVLERLEEMSCNARELPAEAKAGMPRIDWCTWEHLPDTLAGSPRE
                     RRDRLTFVIDTLVPDTLVAAEDYRRALQPQASRA"
     gene            complement(3698..5191)
                     /gene="pepD"
                     /locus_tag="PKX01_15225"
     CDS             complement(3698..5191)
                     /gene="pepD"
                     /locus_tag="PKX01_15225"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HMZ77535.1"
                     /translation="MNAPDRLDPVFDGLSPAPVWAHFAAFCRIPRPSRGEAAIREHLL
                     AWATARDIAAQVDAAGNLILRKPASPGHEGAPGVILQAHLDMVCQANAGTPHDFTRDP
                     IRPVRRDGWLVAEHTTLGADNGIGASLILAALEDDTLIHGPLEALLTVDEEAGMGGAH
                     GLEPGLLNGRLMLNLDTEDWGEFYLGCAGGLDVDARATGQADPLPAGWRSLRLSLAGL
                     RGGHSGVNIHEERGNAIKLLVRVLCDLASDAPLRLALLRGGTARNALPREASAVIAVP
                     AAVADGLPARVAAWQALLQDELRGVEAGLSLHLDDAAADSVLAVADQARWLNALHAAP
                     HGVRRWSLDVPGVVETSNNLGIVELTPNGGHANFMVRSLRDSGSAALAAELLSLLTLA
                     GLPATASGHYPGWTPNPDSPLLALCQRVFTRRFGGTSTVKVVHAGLECGLIGARYPGL
                     DTVSFGPTIRGAHAPGERVEIASVDAAWQLLGALLAALARGELPAPA"
     gene            5336..6373
                     /locus_tag="PKX01_15230"
     CDS             5336..6373
                     /locus_tag="PKX01_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HMZ77536.1"
                     /translation="MVRIAINGYGRIGRCFLRALRASPWRDALQVVAINEPADLDSMA
                     YLTRFDSTHGVFPGEVTVQDGGLSIDGQAIRVFHADTPEGVAWAEAGVDLLVEASGRY
                     STRAELERFVAAGCPRVLLSHPGHSAADVDRTIVYGMNHALLRGDERLVSAASCTTNA
                     IVPVLALLDEAFGIEHALMTTLHSVMNDQPLIDGYHHADLLRTRSAMQSLIPVSTGLA
                     RGVQRLLPELAGRIEAKAIRVPVVNVSAIDLVATLRRDADRGAVQALLRDAAAGPGAG
                     RMAYTDEPHASIDFNHSPHSAIVDGGQIRLVGGRLLNLLVWFDNEWGFANRMVELAGH
                     WARRMDAPGAG"
     gene            complement(6377..>7263)
                     /locus_tag="PKX01_15235"
     CDS             complement(6377..>7263)
                     /locus_tag="PKX01_15235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HMZ77537.1"
                     /translation="VAVVGAGPAGLAAATTLAERGHEVSLFDAADTVGGQFNLARRIP
                     GKEDFGETLRYFRVRLAELDVALRLGHTVTAADLAGFEHVVLATGITPRAPAIPGVDH
                     PKVTSYIDVLEGRRSVGPRVALIGAGGIGHDVAEFLTHAHDGSDPITAYRNEWGIDAA
                     YTDNRGGLTAPRGPAVPRQVWLLQRKASRVGAGLAKTTGWARSLLLRKRGVHMLGGVV
                     YLRIDDAGLHLRVGDADQVLDVDTVVICAGQEPRRDLAAGLEAAGIPYTLVGGADVAA
                     ELDAKRAIAQATEFARRF"
BASE COUNT         1129 a         2514 c         2540 g         1080 t
ORIGIN      
        1 gcgacggact ggatcaggtc ttcctgtttg atggcggtca tgggtgaggc tccctctcgt
       61 tagtgtttgg acagcaggac ggtctggttc atgagcttgt ccaccagcgc catggccagg
      121 gcggacagca ggaatacgac gtggatgatc acctgccact tgatggtgtg ctcgggcagg
      181 ttgccggcgt tgatgaaggt cttgagcagg tggatcgacg agatgccgat gatggcggtg
      241 gacagcttga tcttgagcac gccggcgttc acgtgggaga gccactccgg ctggtccggg
      301 tggttgtcca gatcgaggcg cgagacgaag gtctcgtagc cgccgatgat caccatgatc
      361 agcaggttgg cgatcatcac cacgtcgatc aggccgagca ccaccagcat ggtctcggtc
      421 tcgctgatgt ggccgccgga ttcgaagacc gagccgatca ggtgggccag ttcggccatg
      481 aacagataga catagacgcc ttgcgcggcg accagcccca ggtagagggg cgcctggaac
      541 cagcggctcc agaagatgaa gcgttcgagg tgctgttggc ctggactcat gggaaaaact
      601 cggagtgaag gacggcggat tttatcatcg gcgcaggcgc ttccgagacg ttaaaatgtc
      661 ggactttgct tttccgcctc aagggctttc cgcatgaacc tgcaccccat caccgcgctg
      721 tcgcccctgg acggccgcta cgaatccaag gtcgccgccc tgcgcgagca cttctccgaa
      781 ttcggcctga tccgcaaccg ggtcaaggtt gagatcgagt ggctcaaggc cctcgccgcc
      841 gcgcccgaac tcgccgagat cgcgcccttt tccgccgcca ccgtggccga gctggacggc
      901 gtgatcgccc acttttcggt gaccgacggc gaggccgtga aggccatcga agccaccacc
      961 aaccacgacg tgaaggcgat ggagtactgg ctcaagcagc gcctgggcca caacccggaa
     1021 gtcacccgcg tcaccgagtt catccacttc ggctgcacct cggaagacat caacaacgtc
     1081 tcccacgccc tcatgctgcg cgacggccgc gacggcgtgc tgctgccggc cctcgacagg
     1141 atcatcgccc gcctcgtcga gctggcccac aacctggccg acctgcccat gctgtcgcgc
     1201 actcatggcc agcccgccag cccgaccacc ctgggcaagg agatggccaa cgtggtgcac
     1261 cgcctgcgcc gcgtccgcgc gtccatcgcc ggcgtcgagc tcaccgccaa gttcaatggc
     1321 gccgtgggca attacaacgc ccacctgtcc gcctaccccg acttcgactg ggaaggcttc
     1381 aacggccgct tcatcgagtc cctgggcctg acgttcaatc cctatacgat ccagatcgag
     1441 ccccatgacg cgatggccga gctctacgat gccacggccc gcgccaacac catcgtcctc
     1501 gacctgtgcc gcgacatctg gatgtacatc tccctgggtt acttcaagca gaagctcaaa
     1561 gccggcgaga tcggctcctc caccatgccc cacaaggtca accccatcga cttcgagaac
     1621 gccgaaggca acctgggcct ggccaacggc gtgctgcgcc acctggccga caagctgccg
     1681 gtgtcgcgca tgcaacgcga cctcaccgac tccacggtgc tgcgtaacat gggcgtggcc
     1741 ttcggctacg cggtgctggc ctacgattcc tgcctgcgcg gcctgggcaa gctggaggcc
     1801 gatccggccc gccttgccgc cgatctggac gcctgctggg aagtgctcgc cgagcccatc
     1861 cagaccgtca tgcgccgcta cggcgtgccc aacccctacg agcagctcaa ggagctgacc
     1921 cgcggcaagg gcatcacccg cgacgcgctg caggccttca tcgccaccct ggcgattccg
     1981 gaggccgaaa agacccgcct gctcgccatg acgcccgcca gctacatcgg caaggccgcc
     2041 gagctcgccc gccgcatctg aaccccgtac gggctgccgt atgcgccgcg tgcgcagccc
     2101 gacgaccgga gacctggaat ggctttcgcc gacaaactga agaccctgcc cggcgtgtct
     2161 cacctggcag cgctgcacct cctcgacggc gctggccagg tggttggcac catcgagaac
     2221 aagcccggcc aggccggctc cctggcggtg tacaaccacc tggcccagct tcacggcgcc
     2281 atcaccccgg acgccgccac ccagggcatc gagctctacg ccgagcacag cgaggatgcg
     2341 cgcctcaacc ctggcaagca ccccaacatc gaccgcctga tcggtctggt ggagcggggc
     2401 gagacgctgc gggtcaagca cgtcttcgcc gcctgagcgc tgcgcgcgct ggcatggcgg
     2461 gccgcctgcg ggcggccttt ttcgtggatg cggggctcag gctctggcta tattcaccac
     2521 gcgacgggca aaccagccgg ccagacgcag tccgatgaac accccgaccg aatccgccag
     2581 ccagtcccac gggtcgccga cgcgattggt cgtgaagcgg tgctggcaca cttcgatcag
     2641 caggccgtag gccaccagcc cggcggccac cgccgtccgg cgcaggggga acccggtcac
     2701 gcccatcagg gtcagcagga cgaaggcgcc ggtgtgttcc accttgtcct gaaaggagaa
     2761 cacggtcagg gcttcgggca gcgggatcat cgccagccag aagatcacga ccagggccag
     2821 cgcaaaggct gtgcggacga cggggggcgg aaagcgcagg ggcatcggca gggcgaagga
     2881 accggggacg gcggattcta gcgcggacgc ggcgccggtc gcccgaccgt tctcatggca
     2941 tctgtcacag ggtgttcacg cggacggtgt tagatgacgc cccaatgctc atgaacgctg
     3001 caaagcaccg ctgaaatgat gggtttgtac tgtctttcgg cgggcctaca ctgcctgggc
     3061 ggcaccgcgt ggacgccgcc tgtcgcgagc ggagggcgat gcagtgacga tgtcatctgc
     3121 gcagtgacaa atgtcaccgc cgtccagtga cggctgacct atcctgccag cccggttctc
     3181 cggcacactg ccgctcaacc ggaaatcagg gtgaccggcg tctgccggta cccggccgga
     3241 acctcgaagg cccggtgcaa ctcgaaggtg tcccatgtcc cgaatccatt cccgtcggat
     3301 cgtcccgtcg tccaagcgcg cccaggccgc ctgggtgctc tatggcgcct tgctgggcaa
     3361 cctgcacgac gccggggagg ctatcgaacg ctgcgccgcc gaccgggggc gggacgccct
     3421 gttccagtct caggccctgt gggagtccgt gctggagcgc ctggaggaga tgagctgcaa
     3481 tgcccgcgaa ctgccggctg aggccaaggc gggcatgccg cgtatcgact ggtgcacctg
     3541 ggaacacctg ccggacaccc tggcgggctc gccacgggag cgccgcgacc gcctcacttt
     3601 cgtcatcgat accctggtgc cggacaccct cgtcgctgcc gaggactatc gtcgggccct
     3661 gcagccgcag gcgtcccgcg cctgaccccg gcacccctca cgccggcgcg ggcagctccc
     3721 cgcgggcgag cgcggccagg agcgcgccca gcaactgcca ggcagcatcc accgacgcga
     3781 tctccacccg ttcgcccggc gcgtgggcgc cgcggatggt ggggccgaag gagaccgtgt
     3841 ccaggcccgg atagcgtgcg ccgatgaggc cacattcgag ccccgcgtgc accaccttca
     3901 ccgtggacgt cccgccgaag cgacgtgtga acacccgctg gcacagggcc agcagcggcg
     3961 aatccggatt cggcgtccag cccggatagt ggccgctcgc ggtggccggc aggccggcaa
     4021 gggtcagcag gctgaggagc tcggcggcga gggcggcgct gccgctgtcg cgcagggagc
     4081 gcaccatgaa attggcgtga ccgccgttgg gcgtcagttc cacgatgccc agattgttgg
     4141 aggtttccac cacgccgggt acgtccaggc tccagcgccg caccccgtgg ggcgctgcgt
     4201 gcagggcgtt cagccagcgg gcctggtcgg cgaccgcgag cacgctgtcg gcggcagcgt
     4261 cgtcaaggtg caggctgagg ccggcttcca cgccgcgcag ttcgtcctgc aagagggcct
     4321 gccaggcggc cacgcgcgcg ggcagcccgt cggcgacggc ggccggtacg gcaatcactg
     4381 cgctggcttc gcggggcagg gcattgcggg cggtgccgcc gcgcagcagg gcgagacgca
     4441 gcggcgcgtc cgatgccagg tcgcacagca cgcggaccag caacttgatg gcgttgcccc
     4501 gctcttcgtg aatgttcacg ccggaatggc cgccgcgcag gcccgccagg ctcaggcgca
     4561 ggctgcgcca gcccgccggc agcgggtcgg cctggccggt ggcacgggca tccacgtcca
     4621 gcccgccggc gcagcccagg tagaactcac cccagtcctc ggtatccagg ttgagcatca
     4681 ggcggccgtt cagcaggccg ggttcgaggc cgtgggcgcc ccccatgccg gcctcttcat
     4741 ccaccgtgag gagggcttcg agggggccgt ggatcagcgt atcgtcctcc agcgctgcca
     4801 ggatcagcga cgcgccgatg ccgttgtcgg cgccgagggt ggtgtgctcc gccaccagcc
     4861 agccgtcgcg ccgtacgggc cggatcggat cgcgggtgaa gtcgtggggc gtgccggcgt
     4921 tggcctggca gaccatgtcc aggtgcgcct gcaggatcac ccccggggcg ccctcgtggc
     4981 ctgggctggc cggcttgcgg aggatcaggt tgccggcggc gtccacctgg gcggcgatgt
     5041 cgcgggccgt tgcccaggcg aggaggtgct cgcggatcgc cgcctcgccg cgggaggggc
     5101 gcgggatgcg gcagaaggcg gcgaaatggg cccacacggg ggcggggctg aggccgtcga
     5161 agacggggtc gaggcggtcc ggggcgttca tggcagggct tcgtggggac tgggagtggg
     5221 cgggcgacag gcccgcgccg gcgccattat cgcccagcct gccgggcagg gacgggccgg
     5281 agggtgtagg cttgcgcctt tttcagcagg gcggaaaggc gtgcacggtt cggacatggt
     5341 gaggattgcg atcaacggct acgggcgcat cgggcggtgt ttcctgcggg cgctgcgggc
     5401 gtcaccgtgg cgggacgcct tgcaggtggt ggccatcaac gagccggcag acctggacag
     5461 catggcctac ctgacgcgct tcgactccac ccacggcgtg ttccctggcg aggtgacggt
     5521 gcaggacggc ggcctgagca tcgacgggca ggccatccgc gtcttccacg ccgacacgcc
     5581 ggagggggtg gcctgggccg aggcgggcgt ggatctgctg gtagaggcct ccgggcgcta
     5641 cagcacgcgg gccgagctgg agcgcttcgt tgcggcgggc tgcccgcggg tgctgctgtc
     5701 tcaccccggg cacagcgcgg cggacgtgga tcgcaccatc gtctatggca tgaatcacgc
     5761 gctgctgcgc ggcgatgagc gcctcgtgtc ggcggcctcg tgcaccacca acgccatcgt
     5821 gccggtgctg gccctgctgg acgaggcctt cggcatcgag cacgcgctga tgaccaccct
     5881 ccattcggtg atgaacgacc agccgctgat cgacggctac catcacgccg acctgctgcg
     5941 cacccgctcg gcgatgcagt ccctgattcc ggtgtccacc ggcttggcgc ggggcgtgca
     6001 gcgtctgctg ccggagctgg ccgggcgcat cgaggccaag gcgatccgcg tgccggtggt
     6061 gaatgtgtcg gccatcgacc tggtggcgac cctgcgccgc gacgcggacc gtggggcggt
     6121 gcaggccctg ctgcgggacg ccgccgccgg ccccggcgcg gggcgcatgg cctacacgga
     6181 tgagccccac gcctccatcg acttcaacca cagcccccat tcggccatcg tggatggcgg
     6241 ccagatccgc ctggtgggcg ggcggttgct caacctgctg gtgtggttcg acaacgaatg
     6301 gggcttcgcc aaccgcatgg tggagctggc tgggcactgg gcccggcgca tggacgcgcc
     6361 gggcgccggc tgagcgtcag aaacggcggg cgaactcggt ggcctgggcg atggcgcgct
     6421 tggcgtccag ctcggccgcc acgtcggcgc cgcccaccag ggtgtagggt atgccggcgg
     6481 cctccaggcc ggccgccaga tcgcggcgcg gctcctggcc ggcgcagatc accaccgtat
     6541 ccacgtccag cacctgatcc gcatcgccca cgcgcaggtg caggccggcg tcgtcgatgc
     6601 gcagatacac cacgccgccc agcatgtgca cgccgcgctt gcgcaggagc aggctgcgcg
     6661 cccagccggt ggtcttggcc aggcccgcgc cgacgcggct ggccttgcgc tggagcagcc
     6721 agacctggcg cggcacggcc ggcccccggg gcgcggtgag gccgccccgg ttgtccgtgt
     6781 aggcggcgtc gatgccccat tcgttccggt aggcggtgat ggggtcgctg ccgtcgtggg
     6841 cgtgggtgag gaactccgcc acgtcgtggc cgatgccgcc ggcgccgatc agggcgaccc
     6901 gcgggcccac gctgcggcgg ccttccagca catcgatgta gctcgtcacc ttggggtggt
     6961 ccacgccggg aatggcgggc gcgcgcggcg tgatgccggt ggccagcacc acgtgctcga
     7021 agcccgccag gtcggcggcc gtcacggtgt ggcccaggcg cagcgccacg tccagctcgg
     7081 ccaggcgcac gcggaaatag cgcagggtct cgccgaaatc ctccttgccg gggatgcgcc
     7141 gggccaggtt gaactggccg ccgacggtgt cggccgcgtc gaacaggctg acctcgtggc
     7201 cgcgttcggc gagggtcgtg gcggcggcca gaccggccgg gccggcgccc accacggcca
     7261 ccc
//
LOCUS       DAOQPA010000148         7036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6056_length_7036_cov_14.497780, whole genome shotgun sequence.
ACCESSION   DAOQPA010000148 DAOQPA010000000
VERSION     DAOQPA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_7036_cov_14.497780"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PKX01_15240"
     CDS             <1..707
                     /locus_tag="PKX01_15240"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ77538.1"
                     /translation="AAREGSGLPLPRLDAAPPIAEAYVLQPQPPVGLRDLAVLWRRYR
                     RTTRNGPRSELDLAATLRERCRRGILCRPVCRPRRGNSARLLILADASPSMDPWRPLF
                     AGLAASLPLGRLAQAGLHYFANLPQARLYATPSLDRSEDLDALLRRHAGAGLLIVSDA
                     GSARGALNRRRARQTVDFLDAARRRFAQVVWLNPMPRHRWAGTTAALIAAGPVPMLPL
                     DAPQLLRAIDLLRGTR"
     gene            712..4176
                     /locus_tag="PKX01_15245"
     CDS             712..4176
                     /locus_tag="PKX01_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ77539.1"
                     /translation="MTTPAALGQHPLDVHEAYEALARYARDYGRAPLLLVMHAAVPET
                     LRPDLLNLIRVNFLAAQGADPSLEADVLFAPFTTALGGGYYRIDPQVRWHALALLRSL
                     YRHDARPRPRRIAELLWRYVEARQHQASRAADPQLAEFLDIQRWVALAFLDPTGAARA
                     FADALQQAGHASGSVALRLGGLASAIELPLSGQPELIAYARGLDALAGGNDADAERLL
                     GALGDGELRIGDVVLRAPTAVLAEGKGQTATADAGADAPPKRRTCLVITGFGRKTDPA
                     TGRVLDLDQSYAMIRSAVEAAGMACVRADGGEATVLGAAHLFQQILASDLVICDLSCR
                     DGNTLYLAGVSLALRPRGILLIADDGFAEYASAAAPRLGLTYASAGDALAPDVQARFS
                     ASLRALVDGLFSAELDLPGSQHRPLGSPLYASGHLQPPRMLDAPSPAAAWAAPPSGSE
                     RLCFIVQGAGTKTDLTTGRTLDLDATYAVVRDAVTSVGLTPRRGDEVVHSGHLESDLH
                     DFLLDAPFVISDLTAGLPEVLIQLGLRHGLRPGRTLLIAEEQFKLPVELASLRILRYK
                     HLGTDIGAQEARRLQAAIVDWLKAAMASPTVDSPVYAALPTLRPPQRIEAADPPDAEP
                     AMAEAPPEAGAASGPAADEAAPAPPPRVFLSHYQGYLQYALPLAKALHSVGIEVSWYM
                     NLSSGEDFAETLALWLEESDVVIAVVGRQTSNAKNALNEIHRAIALGKRLVPVFVDEG
                     APSEVLSFMAANVDASGHIAYLAGLKDLALDQALAHAAVRIAAALKPAAPTPEPAAED
                     APAEPAATSADPMPLIWEITIRQRASGRLHFRVTTADGQHEWQVGYQPALVQPLLNAL
                     QTSTRLDVQAAQTLAHLLIPVEWARMAPRTAYRLTVGRETAALPWELLLEDTLWTREL
                     RDPPRVLRHLDGLRRNPGVALASGSTCALVIGDPDTSGMSTTHPPARLPGARQEAETV
                     ADTLEEAGFQVTRSLGEGASAVIGKLYGAPFRLLLASGSAVRDYALPDGNRVTGLALG
                     DDVFLTVHELRQLRQLPEVVVLNACHLGQVGADAESATADLVPDLLQAGVPVVVAPAW
                     SVDDMAAAAFSKAFFAALAGNARLEDAVANARIAAARAAPETSTWAAYQAWGDPDFRL
                     FPTAPAAE"
     gene            complement(4178..5077)
                     /gene="rarD"
                     /locus_tag="PKX01_15250"
     CDS             complement(4178..5077)
                     /gene="rarD"
                     /locus_tag="PKX01_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HMZ77540.1"
                     /translation="MNPPPDRRTTLAGIACGLAAYGMWGFFPLFFRQLGHVSPVDLLC
                     NRALWGCVFVSLILTVRRAWAGPLAAARRPGTLPRLTLAALLVGANWLAFLWAVDQHR
                     VIEGSLGYFLTPLVNVLLGLVVLKERLKRLEWASVALAVAAVGNEFLSLGSLPWVSLF
                     LGGTFGLYGLVRKQVPVDAVSGLWLETLSLMPLILLYAGWQASRGHGVFTGFDASTQI
                     LLAVAGVMTALPLMFFAAATQRLNLATVGMLMYINPTLQFLTAVLIFGEALRPARLVT
                     FALIWAGLLLYSWSGWQKYRAAR"
     gene            5208..5528
                     /locus_tag="PKX01_15255"
     CDS             5208..5528
                     /locus_tag="PKX01_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HMZ77541.1"
                     /translation="MLVIFKSPASGDVIMFEKNGKEMLGVLGKDPADAKGIVTVEQLP
                     GAIATLRQAIADDKARHAGQDDVQEAEPKSGGPGVSFFQRAVPLLDLMERSLKDKVPV
                     TWGV"
     gene            5536..5910
                     /locus_tag="PKX01_15260"
     CDS             5536..5910
                     /locus_tag="PKX01_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77542.1"
                     /translation="MTQRDIELALQLVREALPHLAVPRRLCTRRLKPGSRAMGQYRWL
                     SDTLRLNPRYLARLSDPDALDLLDTILHELLHKASPLWKQVRDTFRPHPDIWLDAERL
                     TAQLGPRFLHLRRADARRHRTA"
     gene            complement(5962..6744)
                     /locus_tag="PKX01_15265"
     CDS             complement(5962..6744)
                     /locus_tag="PKX01_15265"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HMZ77543.1"
                     /translation="MLTGRTALVTGSTSGIGLAIARALAQAGAKVMLNGSRPAAEAEA
                     LRAALAEETGAEIGYTAADLTDGAGARGLVEATLAAFGQLDILVNNAGMQHVAAVDVF
                     PDEKWDALIALNLSSVFHTTKAALPAMKARGWGRIVNIASAHGLVASPYKSAYTASKH
                     AVVGFTKAVALEVAEEGITCNALCPGYVRTPLVEKQVADQAKVHNLPPERVIREVILA
                     AQPTKEFVEADEVAAYAVFLCSQAGRSVTGSAPLIDGGWTAR"
     gene            6823..>7036
                     /locus_tag="PKX01_15270"
     CDS             6823..>7036
                     /locus_tag="PKX01_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HMZ77544.1"
                     /translation="MHELFSHRPRTLAEAERQIAAIQARLAARGIPHRAPPPPPDTCC
                     GRGCNGCVWEGYYAALAWWREEAAERL"
BASE COUNT         1049 a         2821 c         2233 g          933 t
ORIGIN      
        1 ccgccgcccg cgaaggcagc ggcctgcccc tgccgcgcct cgacgccgca ccgcccatcg
       61 ccgaagccta cgtcctccag ccccagcccc cggtgggcct gcgcgacctg gcggtgctgt
      121 ggcgccgcta ccggcgcacc acccgtaacg gcccacgcag cgagctcgac ctggccgcca
      181 ccctgcggga gcgctgccgg cgcggcatcc tgtgccggcc cgtgtgccgg ccgcgacgcg
      241 gcaacagcgc ccgcctgctg atcctcgccg acgccagccc cagcatggac ccgtggcgcc
      301 ccctcttcgc cggcctcgcc gcctccctgc ccctcggccg cctcgcccag gccgggctgc
      361 actacttcgc caacctgccc caggcccgcc tctacgccac cccgtccctc gaccggtcgg
      421 aagacctgga cgccctgctg cgtcgccacg ccggcgccgg cctgctcatc gtcagcgatg
      481 ccggcagcgc ccgcggcgcc ctcaaccgcc gtcgcgcccg ccagaccgtg gatttcctcg
      541 acgccgcacg ccgtcgcttc gcccaggtgg tgtggctcaa ccccatgccg cgccaccgct
      601 gggccggcac caccgccgcc ctcatcgccg ccggcccggt gcccatgctg cccctcgacg
      661 ccccgcaact gctgcgcgcc atcgacctgc tgcggggcac acggtgacgc catgaccacc
      721 cccgccgccc tcggccagca cccgctcgac gtccacgaag cctacgaggc cctcgcccgg
      781 tacgcccgtg actacggtcg cgcgcccctg ctgctcgtga tgcacgccgc cgtgcccgag
      841 accctgcggc cggacctgct caacctgatc cgcgtgaact tcctcgccgc ccagggcgcc
      901 gaccccagcc tggaggcgga cgtgctgttt gcgcccttca ccaccgccct cggcggcggc
      961 tactaccgca tcgaccccca ggtgcgctgg cacgccctgg ccctgctgcg ctccctctac
     1021 cgccatgacg cccgcccccg cccgcgacgc atcgccgagc tgctgtggcg ctacgtggag
     1081 gcccgccagc accaggccag ccgcgccgcc gacccgcaac tggccgagtt cctcgacatc
     1141 cagcgctggg tcgccctggc cttcctcgac cccaccggcg ccgcccgcgc cttcgccgac
     1201 gccctccagc aggccggcca cgcctccggc agcgtcgccc tgcgcctggg cggcctcgcc
     1261 tccgccatcg aactgcccct ctccggccag cccgaactca tcgcctacgc ccgcggcctc
     1321 gacgccctcg ccggcggcaa cgacgccgac gccgaacgcc tcctgggcgc cctgggcgac
     1381 ggcgaactgc gcatcggcga cgtggtgctg cgggcgccga cggcggtatt ggcggagggg
     1441 aaagggcaga ccgcgacggc ggacgccggg gcggacgcgc cgccgaagcg gcgaacctgc
     1501 ctggtcatca cgggtttcgg gcgcaagacc gatcccgcca cgggccgcgt cctcgacctg
     1561 gaccagagtt acgcgatgat ccgcagcgcc gtggaggcgg ccggaatggc gtgcgtacgg
     1621 gccgacggcg gtgaagccac cgtcctcggc gccgcgcatc tgttccaaca gatcctcgcc
     1681 agcgatctgg tgatctgcga cctgagctgc cgagatggca acacccttta tctggccggc
     1741 gtgagcctgg cgctgcgtcc gaggggcatc ctgctgatcg ccgatgatgg cttcgccgag
     1801 tacgcctcag ccgccgcgcc gcgcctgggc ctcacctacg cttccgcagg cgacgccctc
     1861 gcgccggacg tccaggcgcg cttctcggca tcgctgcgcg cgctcgtcga cggcctgttc
     1921 agcgccgaac tcgatctgcc ggggtcccag caccggcccc tgggcagtcc gctctatgcc
     1981 tcaggacacc tccagccgcc ccggatgctc gacgcgccgt cgcctgccgc cgcgtgggcg
     2041 gctccaccca gcggcagcga acggctgtgc ttcatcgtgc agggcgcggg caccaagacc
     2101 gatctcacga ccggacggac actggatctc gacgccacct acgcggttgt ccgcgatgcc
     2161 gtgacgagcg tcggcctgac gccccggcga ggcgacgagg tcgtccattc cgggcatctc
     2221 gaatccgacc tccacgactt cctgctggac gcccccttcg tcatctccga tctcaccgcc
     2281 ggtctgccgg aggtgctgat tcaactcggc ctccgccacg gcctgcgtcc cggccggaca
     2341 ctgctgatcg ccgaagaaca attcaagctc cccgtcgaac tcgcctccct ccgcatcctc
     2401 cgctacaagc acctgggcac cgacatcggt gcccaggaag cgcgccgcct gcaggccgcc
     2461 atcgtcgact ggctcaaggc cgcgatggcc tcgcccaccg tcgacagccc ggtctatgcc
     2521 gccctgccca cgctccgccc gccgcagcgc atcgaggccg ccgacccgcc cgacgccgag
     2581 cccgcgatgg ccgaagcgcc cccggaagcc ggggccgcct ccggcccggc cgcggacgaa
     2641 gcggcgcccg caccgccgcc tcgcgtcttc ctgtcccact accaaggcta cctccaatac
     2701 gctttgcctc tggccaaggc gctacacagc gtgggcatag aagtatcgtg gtacatgaat
     2761 ctgagctctg gtgaagactt cgccgagacc ttggccctct ggctggaaga aagtgacgtg
     2821 gtgattgccg tagtcggccg tcagacctcg aatgccaaga acgcattgaa tgagattcat
     2881 cgagcaattg cgctcggcaa gcgcttggta ccggtcttcg tggacgaggg cgcaccgtcg
     2941 gaagtgctgt ccttcatggc cgccaacgtg gacgcctcgg gccacatcgc ctacctcgcc
     3001 ggcctcaagg acctggccct ggaccaggcc ctcgcccatg ccgcggtgcg catcgcagca
     3061 gcgctgaagc ccgcggcacc cacgccggaa cctgccgccg aggacgcccc cgcggagccc
     3121 gccgccacgt ctgcagatcc aatgccgctg atctgggaaa tcaccatccg ccagcgggcc
     3181 agcggccgcc tgcacttccg ggtcaccacc gccgacgggc agcacgaatg gcaggtcggc
     3241 taccagccgg cactggtgca gccgctgttg aacgcgctgc agacgtccac acgcctggac
     3301 gtccaggcgg cccagaccct cgcccacctg ctgatcccgg tcgagtgggc gcgcatggcg
     3361 ccgcgcacgg cctaccgcct gacggtgggt cgcgagaccg cggcgctgcc ttgggagctg
     3421 ctgctcgagg acaccctgtg gacccgtgaa ctgcgcgatc cgccgcgggt cctgcgccac
     3481 ctcgatggcc tgcgccggaa ccccggcgtg gccctcgcca gcgggtccac ctgcgccctc
     3541 gtcatcggcg acccggacac ctcgggcatg tcgacgaccc atccgcccgc ccgcctgccc
     3601 ggcgcacgcc aggaggcgga gaccgtcgcc gacaccctcg aagaggccgg cttccaggtc
     3661 acccgcagcc tcggcgaagg cgcctcggcg gtgatcggca agctctacgg cgcgcccttc
     3721 cgcctgctgc tggccagcgg cagcgccgtg cgggactacg ccctgcccga cggcaaccgg
     3781 gtcaccggcc tggccctggg cgacgatgtc ttcctgacgg tgcacgagct gcgccagctc
     3841 aggcagctcc cggaggtcgt ggtgctcaac gcctgccacc tgggtcaggt gggcgcggac
     3901 gccgaatccg ccaccgccga tctggtgcca gacctgctcc aggccggcgt tccggtggtg
     3961 gtggcccctg cctggtcggt ggatgacatg gccgcggcgg ccttcagcaa agccttcttc
     4021 gccgccctcg ccggcaacgc gcgcctggag gatgccgtcg ccaacgcccg catcgctgcc
     4081 gcccgggccg caccggagac ctccacctgg gcggcctacc aggcctgggg cgacccggat
     4141 ttccgcctct tcccgaccgc cccggcggcg gaatgaatca gcgcgccgcc cggtacttct
     4201 gccagccgct ccagctgtag agcagcaatc cggcccagat cagggcgaag gtcaccagcc
     4261 gcgccgggcg cagggcctcg ccgaagatca gcaccgcggt gaggaactgc agggtcgggt
     4321 tgatatacat gagcatgccc acggtggcca ggttgaggcg ctgggtggcg gccgcgaaga
     4381 acatcagcgg cagggccgtc atgacacccg ccaccgccag caggatctgg gtggaggcgt
     4441 cgaagccggt gaacacccca tgcccgcggc tcgcctgcca gccggcgtag agcaggatca
     4501 ggggcatcag cgaaagggtc tccagccaca ggccggagac agcatccacc ggcacctgct
     4561 tgcgcaccag gccgtacagg ccgaaggtgc cgccgaggaa cagggacacc cagggcaggc
     4621 tgcccaggct gaggaactcg ttgcccaccg ccgccacggc cagcgccacc gaggcccatt
     4681 ccaggcgctt gaggcgttcc ttgagcacca ccaggcccag cagcacgttc accagcggcg
     4741 tcaggaagta gcccaggctg ccttcgatga cccggtgctg atccactgcc cacaggaagg
     4801 ccagccagtt ggcccccacc agcagcgcgg cgagggtcag gcgcggcagc gtgcccggcc
     4861 gccgcgccgc cgccaggggc cccgcccagg cgcgccgcac ggtgaggatc aggctcacga
     4921 agacacagcc ccacagggcc cggttgcaca gcagatccac cggcgagacg tggccgagct
     4981 ggcggaagaa caggggaaag aagccccaca tgccataggc ggcgaggccg caggcaatgc
     5041 cggcaagggt cgtacggcgg tcgggaggcg gattcacggg gcgctcgcgc gggggacggc
     5101 cctgcacggt accaccgtcc gccggattgc gcgctcaggg ttttccgcca tcgggacaca
     5161 aagccctaca atgaacgcat ccattccctt tcgaggacgc gcacaccatg ttggtcatct
     5221 tcaagagccc cgccagcggc gacgtgatca tgttcgaaaa gaacggcaag gagatgctcg
     5281 gcgtcctcgg caaggatccc gccgacgcca agggcatcgt caccgtcgag cagctccccg
     5341 gcgccatcgc cacgctgcgc caggccattg ccgacgacaa agcccgccat gccggccagg
     5401 acgacgtcca ggaagccgag cccaagtccg gcgggccggg ggtgagcttc ttccagcgcg
     5461 cggtgccgct gctggacctg atggagcgct ccctcaagga caaggtgccg gtcacctggg
     5521 gcgtgtgaca ccaccatgac ccagcgcgac atcgaactgg ccctgcagct ggtccgcgag
     5581 gcgctgcccc acctcgccgt gccgcgccgc ctgtgcaccc gccgcctcaa gcccggcagc
     5641 cgggccatgg gccagtaccg ctggctgtcc gacaccctgc gcctcaaccc gcgctacctg
     5701 gcgcgcctgt cggatccgga cgcgctggat ctcctcgaca ccatcctcca cgaactgctc
     5761 cacaaggcct cgcccctgtg gaagcaggtc cgcgacacct tccgccccca cccggacatc
     5821 tggctggacg ccgaacgtct caccgcccag ctggggccgc gcttcctgca cctgcgccgg
     5881 gccgacgcgc gtcgccaccg gacggcctga cgacagcgcc gcgaacggcg ccgcccgcac
     5941 acccggcggc gcgcagacgc atcaacgcgc ggtccagccc ccgtcgatga ggggcgccga
     6001 gccggtcacc gagcggcccg cctgggaaca caggaagacc gcataggccg ccacctcgtc
     6061 cgcctcgaca aactccttgg tcggctgcgc cgccaggatc acctcgcgga tcacccgctc
     6121 cggcggcagg ttgtgcactt tggcctgatc cgccacctgc ttctccacca gcggcgtacg
     6181 cacgtagccg ggacacaggg cgttgcaggt gatgccttcc tcggccacct ccagcgccac
     6241 cgccttggtg aagcccacca ccgcgtgctt gctggccgta taggccgact tgtagggcga
     6301 ggccaccaga ccgtgggcgg aggcgatgtt cacgatgcgc ccccagcccc gcgccttcat
     6361 cgccggcagc gccgccttgg tggtgtggaa caccgacgac aggttgaggg cgatcagcgc
     6421 atcccatttt tcgtccggaa acacgtccac cgccgccacg tgctgcatgc ccgcgttgtt
     6481 caccagaatg tcgagctgcc cgaaggccgc cagggtcgcc tccaccaggc cccgtgcacc
     6541 ggcgccgtcg gtcaggtcgg ccgccgtgta gccgatctcc gcgcccgtct cctcggccag
     6601 ggccgcgcgc agggcctccg cttctgcggc cggacgcgaa ccgttgagca tcaccttcgc
     6661 gcccgcctgg gccagcgccc gcgcaatcgc caggccaatg cccgaagtgg accccgtgac
     6721 cagcgccgtt ctgcctgtca gcatggcaat ctcctcgaat ggatggaaca aggccgccta
     6781 aaatacgcgc cccggcgccg ccgcgccatc cgtgtcgccc ccatgcacga gctcttcagc
     6841 caccgcccgc gcaccctcgc cgaagccgaa cgccagatcg ccgcgatcca ggcccgcctc
     6901 gccgcgcgcg gcatccccca ccgtgccccg ccaccgccgc ccgacacctg ctgcgggcgc
     6961 ggctgcaacg gctgcgtgtg ggaaggctat tacgccgcgc tggcgtggtg gcgggaggag
     7021 gcggccgagc gcctcg
//
LOCUS       DAOQPA010000149         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6095_length_7005_cov_17.263022, whole genome shotgun sequence.
ACCESSION   DAOQPA010000149 DAOQPA010000000
VERSION     DAOQPA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6095_length_7005_cov_17.263022"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1788)
                     /locus_tag="PKX01_15275"
     CDS             complement(169..1788)
                     /locus_tag="PKX01_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HMZ77545.1"
                     /translation="MDLNAHLYAIGLPGPRQEGSADAMGFKAWNLARLAAIGLPVPPA
                     FVLGTPLCARHRADPAAFRPHLRTLLETQIERLEAVCGLEFGSERKPLLVSVRSGAAV
                     SMPGMMDTVLDVGLTDATLRGLLRVTGNPRLAWDSYRRLIQQFAEVVHGAPAEPFQTL
                     LHDAIAQAGVSRAQDLDFRDLSRLCRHYQDAYAQTVGQPFPQDPAQQLLDATAAVFDS
                     WMSPRAVAYRRLQGLDDGLGTAITVQRMVFGNAGGTSGAGVGFTRDPASGENRLYLDF
                     RFNAQGEDVVSGRHAAAENGRMALVLPSVLSRLQSVREILEREFGDVQEFEFTVQDGV
                     LYLLQTRAAKRTDWAALRIAVDQVTAGAWSPATALRRLDGVDLAGIVQTRIEAAGTDR
                     ELATGTPASIGVASGVIALDAAAAERFAQTGTECILVRDETRSDDLAGIAAAQGVITA
                     KGSRTAHAAVVARQLGKACLVGCNGLRVDLERRRVVFGTQSLHEGERITVDCGSGRVL
                     AGAVRAFVERPDVWLDQVARWRAIAGPALVG"
     gene            complement(1793..3739)
                     /locus_tag="PKX01_15280"
     CDS             complement(1793..3739)
                     /locus_tag="PKX01_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77546.1"
                     /translation="MTEKARIIDALGEPTLLLPALLGEALGASDRIKYYLSLLQAART
                     SALHPQQPAAPLMAERCAAGIDDERLDTLPARAERDHDGQIHIPDAGILCDHIVRDLD
                     ALLAPIRSAGAEAAVALSARAAALRSQPWCESCDTFDAGQFSRLTSADRDAGDSVHLL
                     VLDAHKELNRLQTRIASEDIDGAAAYDIRPADRALIAAFMRGVNASRPLKLDHPGLGT
                     TATRSGGRLVLQNDIGTTDAHVLVVHVENRRVTLTYTDVHLQRLLFFQSLFAHWAVSW
                     EDTRSRRDRGMEDGLYHVSVGTFSANNDAGLGDYLSFLGSRLVFLIDWNRARKRLRLL
                     LPKKETLDLLHWAADENVGHMAWLRSGGEQLVFDAVAFAARTPPTFGARLDDMLDPGK
                     AVGFMQSVLRACTRGFLERRPDEVVRDEIRAELATCFRSARQQIIDIVAEHAAFSIEI
                     ATGIRDCLPDLTGPDAAARIAANAARARQWEQRADERLNQARELRQQQGEWDDFYHDL
                     VARADDITDELEEAAFHLTLFPPEAPSAELLECFATLAGLAVSGAQEFLKAVETARGL
                     RRGGPREDIQDFLAAIHQILVAERQSDDTHRRFKQCLVATPGEFRSHYPWAEFARNLE
                     TACDALLHAALQLRDHIMLTVTRD"
     gene            complement(3894..4397)
                     /locus_tag="PKX01_15285"
     CDS             complement(3894..4397)
                     /locus_tag="PKX01_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HMZ77547.1"
                     /translation="MATKQEQQFNEIQKKNLESAMRLAQLSIENSQRIMELQVGTAKT
                     LFEEGVANAKALSTAKDPAEVMTLRTAYAQSTTEKMLSCAREIAEITAKTQAEVGKMV
                     TEQLTGGGQDIFESLQKMFKGMPIGDTNALGSIQSAMDTARAAYEQMTKASTEAFQAF
                     TKMSPKK"
     gene            4839..>7005
                     /gene="clpB"
                     /locus_tag="PKX01_15290"
     CDS             4839..>7005
                     /gene="clpB"
                     /locus_tag="PKX01_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HMZ77548.1"
                     /translation="MRFDKLTTKFQQALQEAQSLAVGNDQQFIEPQHLLMALLSQEDG
                     STASLLQRAGVNGQALRAALAKALTRLPKVEGHGGEVSVGRDLGNLLNVTDREAQKRG
                     DEFIASEMFLLALADDKGETGRLLKEHGLARKALEQAIEAVRGGQGVGSAEAESQREA
                     LKKFCVDLTERARQGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLVLDMAGLLAGAKYRGEFEERLKAVLTDIAKDEGRIILF
                     IDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEVMDKLERRKIQLEIEQLAVKKEKDEASQKRLALIEEEIAR
                     IKKEYSDLEEIWKAEKSAVQGSAHIKEEIDRLKADIVRLQREGKLEKVAELQYGTLPQ
                     LEAQLKKAEASGDAKPAHRLLRTQVGAEEIAEVVSRATGIPVSKMMQGERDKLLRMED
                     RLHQRVVGQDEAVRLVADAIRRSRAGLSDENRPYGSFLFLGPTGVGKTELCKTLAEFL
                     FDSEEHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILFDEVE
                     KAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNL"
BASE COUNT         1111 a         2201 c         2619 g         1074 t
ORIGIN      
        1 tcgagcacgg tgattgcccg gacggtgacc gcgacggtga atgcaggcag cgcggcggtg
       61 acgatcagct gaggcggacc cgctggcgtg gccggctgtt cgagggacgg cggccgccag
      121 ccgggctcgc cccggcgcat cggcaccgcc gatcgtggac ccgggccttc agcccacgag
      181 ggcggggccg gcgatggcgc gccagcgggc gacctggtcg agccagacgt ccgggcgttc
      241 gacgaaggca cgcacggcac cggccagcac gcgcccgctc ccgcagtcca cggtgatgcg
      301 ctcgccctcg tgcagggact gggtgccgaa gaccacgcgg cggcgctcca ggtccacgcg
      361 caggccattg cagcccacca ggcaggcctt gccgagctga cgggcgacca ccgcggcgtg
      421 ggcggtgcgg ctgcccttcg ccgtgatcac gccctgggcg gcggcgatgc cggcgaggtc
      481 gtcgctgcgg gtctcgtcgc gcaccaggat gcattcggtc ccggtctggg cgaagcgttc
      541 cgctgctgcc gcgtcgaggg cgatgacccc gctcgccacg ccgatgctgg cgggcgtgcc
      601 ggtcgccagt tcccggtcgg tacccgcggc ctcgatgcgg gtctggacga tgccggccag
      661 atccacgccg tcgaggcgcc gcagggcggt ggcgggcgac catgcgccgg cggtgacctg
      721 gtccacggcg atgcgcaggg cggcccagtc ggtccgcttg gcggcgcgtg tctggagcag
      781 gtagagcacg ccgtcctgca cggtgaattc aaactcctgc acgtcgccga actcccgctc
      841 caggatttcc cgaacggact gcaggcggct gagcacggag ggcaggacga gggccatgcg
      901 gccgttctcg gcggcggcgt ggcgcccgga gaccacgtcc tcgccctggg cgttgaagcg
      961 gaagtccagg tacaggcggt tctcgccgct ggccgggtcg cgggtgaagc cgacgcccgc
     1021 gcccgaggtg ccgcctgcgt tgccgaagac catgcgctgg acggtgatgg cggtgccgag
     1081 gccgtcgtcg aggccctgca gccggcgata ggccacggcg cgcgggctca tccacgagtc
     1141 gaacacggcc gctgtggcgt cgaggagctg ctgcgccgga tcctgcggga agggttggcc
     1201 gacggtctgc gcgtaagcgt cctggtagtg ccggcacagg cgcgacagat cgcggaagtc
     1261 cagatcctgg gcgcggctca ccccggcctg ggcgatggca tcgtgcagca gggtctggaa
     1321 gggctcggcg ggggcgccgt gcaccacctc ggcgaactgc tggatcaggc gccggtacga
     1381 atcccaggcc aggcgggggt tgccggtgac gcgcaggagg ccgcgcaggg tggcgtcggt
     1441 gaggcccacg tcgagcaccg tgtccatcat gccgggcatg gacacggcgg cgccggagcg
     1501 cacggacacc agcaggggtt tgcgctcgga tccgaactcc agcccgcaga cagcctccag
     1561 gcgctcgatc tgggtttcga ggagggtgcg caggtggggc cggaaggccg cagggtctgc
     1621 ccggtggcgg gcgcacaggg gcgtgccgag gacgaaggcg ggcggcacgg gcaggccgat
     1681 ggcggccagc cgcgccaggt tccaggcctt gaagcccatg gcgtcggcgg agccttcctg
     1741 gcggggccct ggcaggccga tggcgtagag gtgggcgttg aggtccatgc gttcagtcgc
     1801 gggtgaccgt gagcatgatg tggtcgcgca gttgcagggc ggcatggagc agggcgtcgc
     1861 aggcggtttc caggttgcgg gcgaactctg cccacggata gtggctgcgg aattcgccgg
     1921 gggtggcgac gaggcattgc ttgaagcgcc ggtgggtgtc gtcgctctgc cgttcggcca
     1981 ccagaatctg gtggatcgcc gcgaggaagt cctggatgtc ctcgcgcgga ccgccgcggc
     2041 gcaggccgcg ggccgtctcg acggccttga ggaactcctg ggcgccgctc accgccagcc
     2101 cggccagggt ggcgaagcac tcgaggagtt cggcggacgg tgcctccggc gggaacaggg
     2161 tgaggtggaa ggcggcttcc tccagttcgt ccgtgatgtc gtcggcgcga gcgaccaggt
     2221 catggtagaa atcgtcccac tcgccctgct gctggcgcag ttcccgggcc tggttgaggc
     2281 gttcgtcggc gcgctgttcc cactggcggg cgcgggccgc gttggcggcg atgcgggcgg
     2341 cggcgtcggg gccggtgagg tcgggcaggc agtcgcggat gccggtggcg atctcgatgg
     2401 agaaggcggc gtgctcggcc acgatgtcga tgatctgctg gcgggcgctg cggaagcagg
     2461 tggcgagctc ggcgcggatc tcgtcgcgta cgacctcgtc cgggcgccgt tcgaggaatc
     2521 cgcgcgtgca ggcgcgcagc acgctctgca tgaagccgac cgccttgccg gggtcgagca
     2581 tgtcgtccag gcgcgcgccg aaggtgggtg gggtgcgtgc ggcgaaggcc acggcgtcga
     2641 agaccagctg ttccccgccg ctgcgcagcc aggccatgtg gccgacgttc tcgtcggccg
     2701 cccagtgcag cagatcgagg gtttccttct tgggcaggag cagacgcagg cgtttgcggg
     2761 cacggttcca gtcgatcagg aagaccagac gcgaacccag gaagctcagg tagtcaccca
     2821 ggccggcgtc gttgttggcg ctgaaggtgc ccaccgagac gtggtagaga ccgtcctcca
     2881 tgccccggtc gcgacgggaa cgggtgtcct cccagctcac cgcccagtgg gcgaagaggc
     2941 tctggaagaa cagcaggcgc tggagatgga cgtcggtgta ggtgagggtg acgcggcggt
     3001 tctccacgtg caccacgagc acgtgggcgt cggtggtgcc gatgtcgttc tgcaggacca
     3061 ggcggccgcc gctgcgggtg gcggtggtgc ccaggccggg gtggtcgagc ttgagggggc
     3121 ggctggcatt gacgccgcgc atgaaggcgg cgatcagggc gcggtcggcg gggcgaatgt
     3181 cgtaggcggc ggcgccgtcg atgtcttcgc tggcgatgcg ggtctggagc cggttgagct
     3241 ccttgtgggc gtccaggacc agcaggtgca cgctgtcgcc ggcgtcccgg tcggcagagg
     3301 tgaggcgcga gaactgcccg gcgtcgaagg tgtcgcagga ttcgcaccag ggctggctgc
     3361 gcaaggccgc ggcgcgggcc gacagggcca cggcggcctc cgccccggca ctgcggatcg
     3421 gcgcgagcag ggcgtccaga tcgcggacga tgtggtcgca caggatgccg gcgtcgggga
     3481 tgtggatctg tccgtcgtgg tcgcgttccg cgcgggccgg cagggtgtcg agacgctcgt
     3541 cgtcgatgcc tgcggcgcag cgttcggcca tcagcggcgc cgcgggctgc tgcgggtgga
     3601 gggcgctggt gcgggccgcc tggagcaggc tgaggtagta cttgatgcgg tcgctggcgc
     3661 cgagcgcttc acccagcagg gcgggaagga gcagggtcgg ttcgccgagg gcgtcgatga
     3721 tgcgggcttt ttccgtcatg gccgcgggcc ggggagatcg tgccgtgcag catgcccgcc
     3781 ccacgtgaca gacgcgtttc aggcggctcc gcctgcccgc cgcgtcgcgc ccgtccgacc
     3841 aaagcaaacg gcgcccgcag gcgccgtcgc ttgatgccgc aaggaagcgt ggattacttc
     3901 ttgggggaca tcttggtgaa ggcctggaag gcttcggtgg aggccttggt catctgctcg
     3961 taagcagcac gggcggtgtc catggcgctc tggatggagc cgagggcgtt ggtgtcgccg
     4021 atgggcatgc ccttgaacat cttttgcagg gattcgaaga tgtcctggcc gccaccggtg
     4081 agctgctcgg tgaccatctt gccgacttca gcctgggtct tggcggtgat ctcggcgatc
     4141 tcgcgggcac aggacagcat cttttcggtg gtgctctggg cgtaggcggt gcgcagggtc
     4201 atgacttcgg ccggatcctt ggcggtggac agggccttgg cattggcaac gccttcttca
     4261 aacagggtct tggcggtgcc gacctgcagt tccatgatgc gctgggagtt ttcgatggac
     4321 agctgcgcca gacgcatggc ggattcgagg ttcttcttct ggatttcgtt gaactgctgt
     4381 tcttgcttgg tagccatgtt gatctccact tcgtttggtt gatgtgagtc cgaaacccag
     4441 atcgatttcc ctcccccggg cttgcacaaa aacgatagca caggctcatg caccgacaat
     4501 gggcgcagat caaaaaaacg tcgatttttt cgatttatgt tgcaccgcag caacaccttt
     4561 ttaaagaacg gtttgcaagg ctctccgggg cggcgggcgc ggtacccgca gcgcggcccg
     4621 gcaatgcccc gaattgaggc atcgggtggg ggcgcaggcg ggggccggac gatgtaacac
     4681 cccctgccac gggaatctgg agcagtggtc ggacggcagg cgttccggcg gcgcggtttg
     4741 ccacggcggc gtgtgaacgg tcttgcccgt gggcgccccc cattgaaact gccggcgcct
     4801 cccccatcta ggcctcagac ccgttcagga ggcattccat gcgtttcgac aagctcacca
     4861 ccaagttcca gcaagccctg caggaggcgc agagcctggc tgtggggaac gatcaacagt
     4921 tcatcgagcc ccagcacctg ctgatggccc tgctgtccca ggaggacggc agcaccgcgt
     4981 cgctgctgca gcgcgccggc gtcaacggtc aggccctcag ggccgcgctg gccaaggcgt
     5041 tgacccgcct gcccaaggtg gaaggccatg gcggcgaggt gtccgtcggg cgcgacctgg
     5101 gcaatctgct caacgtgacg gaccgcgaag cccagaagcg cggtgacgag ttcatcgcca
     5161 gcgagatgtt cctgctggcc ctcgccgacg acaagggcga gaccggtcgc ctgctcaagg
     5221 agcatggcct cgcccgcaag gccctggagc aggccatcga ggcggtgcgc ggcggccagg
     5281 gcgtgggcag cgccgaggcc gagagccagc gcgaggctct caagaagttc tgcgtggacc
     5341 tcaccgagcg ggcccgccag ggcaagctcg atccggtcat cggccgggac gacgagatcc
     5401 gccgcgcgat ccagatcctg caacggcgca ccaagaacaa tccggtgctg atcggcgagc
     5461 ccggcgtggg caagactgcc atcgtggagg gcctggccca gcgcatcgtc aatgacgagg
     5521 tgcccgagac gctcaagggc aagcgcgtgc tggtgctgga catggccggg ctgctcgccg
     5581 gcgccaagta ccggggcgag ttcgaggagc gtctgaaggc ggtgctcacc gacatcgcca
     5641 aggacgaggg ccgcatcatt ttgttcatcg acgagatcca cacgatggtc ggcgccggca
     5701 aggccgaggg cgccatcgac gcgggcaaca tgctcaagcc ggctctggcc cgcggcgagc
     5761 tgcactgcat cggcgccacc acgctggacg agtaccgcaa gtacatcgag aaggacgccg
     5821 ccctggagcg ccgcttccag aaggtgctgg tggacgagcc ctcggtcgag tcgaccatcg
     5881 ccatcctgcg cggtctgcag gagaagtacg aactccacca cggcgtggac atcaccgacc
     5941 cggccatcgt cgccgcggcg gagctctccc accgctacat caccgaccgc ttcctgcccg
     6001 acaaggccat cgacctgatc gacgaggcgg cggcgcgcat caagatggag atcgactcca
     6061 agccggaggt gatggacaag ctcgagcggc gcaagatcca gctggagatc gagcagctgg
     6121 cggtcaagaa ggaaaaggac gaagcctccc agaagcgcct cgcgctgatc gaggaggaga
     6181 tcgcccgcat caagaaggag tattccgatc tggaggaaat ctggaaggcc gagaagtctg
     6241 ccgtccaggg ttcggcccac atcaaggaag agatcgatcg cctgaaggcc gacatcgtca
     6301 ggctgcaacg cgaaggtaag ctcgagaagg tggccgagct gcaatacggc acgctgcccc
     6361 agctcgaagc ccagctcaag aaggcggaag cctccggcga cgccaagccg gcccacaggc
     6421 tgctgcgcac ccaggtgggc gccgaggaga tcgccgaggt ggtgagccgc gccaccggca
     6481 tccccgtgtc caagatgatg cagggtgagc gcgacaagct gctcaggatg gaagaccgcc
     6541 tgcatcagcg cgtggtgggc caggacgagg ccgtgcgcct ggtggccgac gccatccgcc
     6601 gctcccgcgc cgggctgtcg gacgagaacc ggccctacgg ctccttcctg ttcctgggcc
     6661 ccacgggtgt gggcaagacc gagctgtgca agaccctggc cgaattcctc ttcgacagcg
     6721 aggagcacct gatccgcatc gacatgagcg agttcatgga gaagcactcg gtggcccgcc
     6781 tgatcggcgc gccgccgggc tacgtgggct acgaggaggg cggctatctc accgagcagg
     6841 tgcgtcgcaa gccgtattcc gtgattctct tcgacgaggt ggagaaggcc cacccggacg
     6901 tcttcaacgt gctcctgcag gtgctcgacg acgggcgcat gaccgacggt cagggccgca
     6961 cggtggactt caagaacacc gtgatcgtga tgaccagcaa cctgg
//
LOCUS       DAOQPA010000150         6901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6234_length_6901_cov_18.236635, whole genome shotgun sequence.
ACCESSION   DAOQPA010000150 DAOQPA010000000
VERSION     DAOQPA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_6901_cov_18.236635"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKX01_15295"
     CDS             <1..210
                     /locus_tag="PKX01_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMZ77549.1"
                     /translation="GITDAEALQAFHDLCRFEGIIPALESSHALAYAAKLAPTLPKDK
                     ILLVNLSGRGDKDMHTVAEKSGIRF"
     gene            264..1076
                     /gene="trpA"
                     /locus_tag="PKX01_15300"
     CDS             264..1076
                     /gene="trpA"
                     /locus_tag="PKX01_15300"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMZ77550.1"
                     /translation="MSRIQNCFQRLAADGRKALIPFITAGDPAPEMTVPLLQALVAGG
                     ADIIELGVPFSDPMADGPTIQRASERALAAGMSLRKVLDIVRAFRATGDQTPVVLMGY
                     ANPIEAMGVDTFARAAVEAGADGVLVVDYPPEECREFAATCRAAGLDPIFLLAPTSTE
                     QRFADVAAAGSGYIYYVSLKGVTGSAALDLDEVARRIPAIRERVGMPVGVGFGIRDAA
                     TAARIAGLADAVVIGSRIIEEIENSPRDAAPARVTAFLRGVREAMDQRGEAR"
     gene            1073..1945
                     /gene="accD"
                     /locus_tag="PKX01_15305"
     CDS             1073..1945
                     /gene="accD"
                     /locus_tag="PKX01_15305"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HMZ77551.1"
                     /translation="MSWLTKLLPPKIKRDTSSRKAIPEGLWSKCPACEAVLYRSDLES
                     NQNVCPKCGHHQRIRARQRIDLLLDPEGRFEIGAEVVPVDPLKFRDSKRYPERLKAAN
                     EDTGEADALVVVQGAIKTVPVVVACFEFEFLGGSMGSVVGERFVRGVRAALEQRLPFI
                     CATATGGARMQEGLFSLMQMAKTTAAITRLGDRRLPFITLLTDPTMGGVSASFAFMGD
                     VVIAEPGALIGFAGPRVIEQTVRETLPEGFQRSEFLQEKGAVDLIIDRRELRDRLSEL
                     LTLLTRQASVKNAS"
     gene            1998..3296
                     /gene="folC"
                     /locus_tag="PKX01_15310"
     CDS             1998..3296
                     /gene="folC"
                     /locus_tag="PKX01_15310"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HMZ77552.1"
                     /translation="MQWPETLDGWLSLLEVRHDQTIQLGLERVRRVRDALGHPQTCPV
                     FIVGGTNGKGSTCALLEAVLLAAGYRVGLYTSPHLLRYNERVRIDGREASDAELVDAF
                     VEVERARGDVPLTYFEHGTLAAWAAFARAGVEAVILEVGLGGRLDAVNVFEPDCAIVT
                     SVALDHMDYLGSTREAIGFEKAGIFRAGKPAICADPLPPQSLLDHAAAIGAPLWVSGR
                     DFGFAGDQNQWAFWLAQGSRRGGLAYPALRGTNQLLNAAAVMAACEAVKPILPVPMQA
                     IRQGFMLVELPGRFQVLPGRPAVVLDVAHNPQAAGVLNENLASMGFFPETWAVFGMLA
                     DKDVAGVVSLLKDRVDHWRVATLPGPRGLSADGLIALLHDEGVKGDVAGFETPAAAYR
                     AVREQAAEGDRIVVFGSFLTVADVLAGMKVSHQVQQDGGL"
     gene            3283..4074
                     /locus_tag="PKX01_15315"
     CDS             3283..4074
                     /locus_tag="PKX01_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ77553.1"
                     /translation="MADSDSQLDLKKRARRRLVGSAALALTAAIVLPMVMDHEPRPPS
                     QDVQIRIPSQEGSNFTSRVISGKGPAPTQPVAGAADAAVDDGVALPRDRTVPKTVQPE
                     AGAPAGRVDPVRPQAAVESPPDVKPDVRPVPKPDAKPEPRKPEPKPEPKKPEPKPEPK
                     PEPEPKPKPPTDAERAKALLNGEAPTAGANGPHVVLLGAYREAANVTALRARLKAEGF
                     ATYTEPLGDKIRVRAGPFPNKAAADKAAARISKMMGVSASVAVKP"
     gene            4088..4579
                     /locus_tag="PKX01_15320"
     CDS             4088..4579
                     /locus_tag="PKX01_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMZ77554.1"
                     /translation="MNGFDYAMLGLLGLSALLGFWRGLISEVVALGAWVLAFLVAKAF
                     LPDVTPWLAAWVREPVLQAPVAFVVIFVGVLVGVALLRWLLRELIQAAGLGLADRFLG
                     ACFGLVRGGVIVFAVAVLVGLAGLAREAWWREASLSAPLETAVLATRPWLPDPLTKRL
                     RFR"
     gene            4591..6114
                     /gene="purF"
                     /locus_tag="PKX01_15325"
     CDS             4591..6114
                     /gene="purF"
                     /locus_tag="PKX01_15325"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMZ77555.1"
                     /translation="MCGILGVVATTRVNQLLYDGLQVLQHRGQDAAGIATAEGGRFHM
                     HKSSGLVRDVFRTRNMRDLPGNWGIAHVRYPTAGSASSAAEAQPFYVNSPFGIMLAHN
                     GNLTNAEDLKREMFLSDLRHINTNSDSEVLLNVLAHELQAAASSFRLDQDTVFKAVAG
                     VHRRCRGAYATVAMISGTGLLAFRDPYGIRPLVIGRNDTPAGTEWMVASESVALDVLG
                     FRLVRDVAPGEAILIDAEGGFHSRQCATHTVHAPCMFEFVYLARPDSLIDNISVYDAR
                     IKMGEFLARKLRSTMPHLEIDVVIPIPDSSRPSAMEMAHHLNVPYREGFVKNRYIGRT
                     FIMPGLSIRRKSVRQKLNAIHQEFRGKKVLLVDDSIVRGTTSREIVQMARDAGATQVY
                     MASAAPPVRFANVYGIDMPTRHELIASNRTEDEICAEIGADALIYQDLEDLKAAVRAL
                     NPVISVFETSCFDGCYVTGDVTAEYLDGVETQRSEAPSDASEGAAQMDLNLVTGHEG"
     gene            6121..>6901
                     /locus_tag="PKX01_15330"
     CDS             6121..>6901
                     /locus_tag="PKX01_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ77556.1"
                     /translation="MNPLHPETLAVRAGTARSQFNEHSEALYLTSSFVFDSAAQAAAR
                     FSGEEEGNVYARFTNPTVTAMQQRLAALEGAESCVATASGMAAILATVMALMQAGDHV
                     VASRGLFGATQQLFGNILSKFGIETSFVPATDLAAYRAAVTPRTKLFFVESPSNPLTE
                     LVDIGAVAAIAHEAGALLAVDNCFCSPALQQPLALGADLVIHSATKYLDGQGRVLGGA
                     VCGAAHHVDEVFKFLRTAGPCISPFNAWVILKGLETLSIRMA"
BASE COUNT         1080 a         2289 c         2343 g         1189 t
ORIGIN      
        1 ggcatcaccg atgccgaggc gctgcaggcc ttccacgacc tgtgccgttt cgagggcatc
       61 atcccggcgc tggagtcctc ccatgccctg gcctacgctg ccaagctggc gccaaccctg
      121 ccgaaggaca agatcctgct cgtgaatctg tccggtcgcg gcgacaagga catgcacacc
      181 gtcgccgaga agtccggcat ccgtttctga accgaacccg gcggacggcc cgagctgtcc
      241 gccccgattc cacgccatca tccatgtcgc gaatccagaa ctgtttccag cgtttagccg
      301 ccgacgggcg caaggcgctc atccccttca tcacggccgg cgatcccgcc ccggagatga
      361 cggtgcctct gttgcaggcc ctggtggccg gtggtgccga catcatcgag ctgggcgtgc
      421 ccttttccga tcccatggcg gacggtccga cgatccagcg cgcgtccgaa cgggccctgg
      481 cggcgggcat gagcctgcgc aaggtgctcg acatcgtccg tgccttccgg gccacgggcg
      541 accagacgcc ggtggtgttg atgggttacg ccaatcccat tgaggcaatg ggtgtcgaca
      601 ccttcgcccg tgcggcggtc gaggcgggcg ccgacggcgt gctggtcgtg gattacccgc
      661 cggaggaatg ccgtgagttc gcggccacct gtcgcgccgc ggggctcgac ccgatcttcc
      721 tgctggcgcc tacgtccacc gagcagcgtt ttgccgacgt ggcggcggcc ggcagcggct
      781 acatttacta tgtgtccctc aagggcgtga ccggctcggc cgcgctggac ctggacgagg
      841 tggcacgccg catcccggcg atccgcgagc gagtcggcat gccggtgggc gtgggcttcg
      901 gcatccgtga cgccgccacc gccgcgcgca tcgccggtct ggccgacgcg gtggtgatcg
      961 gcagccgcat catcgaagag atcgaaaaca gcccccgcga tgccgctccg gcgcgggtga
     1021 cggccttcct gcgcggtgtg cgcgaggcca tggaccaacg aggggaggcc cgatgagctg
     1081 gctcaccaaa ctgctgccgc ccaagatcaa gcgcgacacc tcgtcgcgca aggcgattcc
     1141 cgaagggctg tggagcaaat gcccggcctg cgaggccgtg ctgtaccgct ccgatctgga
     1201 aagcaaccag aacgtctgcc ccaagtgcgg acaccaccag cgcatccgcg cccgccagcg
     1261 catcgatctg ctgctcgatc cggaaggccg cttcgagatc ggggccgagg tggtgcccgt
     1321 cgatcccctc aagttcaggg attccaagcg ctatcccgag cgtctgaagg ccgccaacga
     1381 ggacaccggt gaggcggacg cgctggttgt cgtgcagggt gccatcaaga ccgtgccggt
     1441 ggtggtcgcc tgcttcgagt ttgaattcct cggcggctcc atgggctcgg tggttggcga
     1501 gcgcttcgtg cgcggcgtgc gggccgcgct ggagcagcgt ctgccattca tctgcgcaac
     1561 cgccacgggc ggcgcgcgca tgcaggaagg gctcttctcc ctcatgcaga tggccaagac
     1621 caccgccgcc attacccggc tgggggatcg ccggctgccc ttcatcaccc tgctcaccga
     1681 tcccaccatg gggggcgtgt ccgcgagctt cgccttcatg ggggacgtgg tgatcgccga
     1741 gccgggcgcc ctgatcggtt ttgccggtcc gcgggtgatc gagcagacgg tgcgcgagac
     1801 cctgcccgaa ggcttccagc gctctgagtt cctgcaggaa aagggggcgg tggacctgat
     1861 catcgaccgt cgcgaactgc gcgaccgcct ctcggaactg ctcaccctgc tgacccgcca
     1921 ggcgtccgtc aagaatgcct cctagcacca cgccgaccac ggcgccgatg tgcggtacgc
     1981 cccggcggga gatctgaatg cagtggcctg aaaccctgga cggctggctg agcctgctcg
     2041 aagtgcggca cgaccagacc atccagcttg gcctggagcg cgtccgtcgc gtgcgcgacg
     2101 cgcttggcca tccccagacc tgccccgtgt tcatcgtcgg cggcaccaac ggcaagggct
     2161 ccacctgtgc gctcctcgaa gccgtgctgc tggccgccgg ctaccgggtc gggctgtaca
     2221 cctcgccgca cctgctgcgc tacaacgagc gcgtgcgcat cgacggccgc gaagccagtg
     2281 atgcggaact ggtggacgcg ttcgtcgagg tcgagcgcgc ccgcggtgat gtacccctga
     2341 cctacttcga gcacggcacc ttggccgcct gggccgcctt tgcgcgggcc ggcgtagagg
     2401 cggtgatcct cgaagtgggg ctgggcggac gcctggacgc ggtcaatgtc ttcgaaccgg
     2461 actgcgccat cgtcaccagc gtggccctgg atcacatgga ttacctgggt agcacccgcg
     2521 aggcgatcgg cttcgagaaa gccgggattt tccgcgccgg caagccggcc atctgcgccg
     2581 atccgctgcc gccccagtcc ctgctggacc atgccgcggc gatcggcgcg cccctgtggg
     2641 tcagcggccg ggatttcggc tttgccggcg accagaatca atgggccttc tggcttgccc
     2701 aaggcagccg tcggggcggc ctggcctatc cggcactgcg tggcaccaat cagctcctca
     2761 acgcggccgc cgtgatggcg gcctgtgagg ccgtcaagcc cattctgccg gtacccatgc
     2821 aggcgatccg ccagggcttc atgctggtgg agctgcccgg gcgcttccag gtgctgccgg
     2881 gccggcctgc cgtggtgctg gacgtggccc acaacccgca ggcggccggc gtgctcaacg
     2941 agaacctggc gagcatgggc ttcttcccgg aaacctgggc ggtcttcggg atgctggcgg
     3001 acaaggatgt ggccggcgtc gtcagcctgc tgaaggatcg tgtggatcac tggcgtgtgg
     3061 ctaccttgcc cggaccacgc ggactgtcgg cggacggact catcgcgctt ctccatgacg
     3121 aaggcgtcaa aggggatgtg gccggcttcg agacgcccgc tgccgcctac cgggcggtcc
     3181 gtgagcaggc agccgagggt gatagaattg tcgtttttgg atccttcctg accgttgcgg
     3241 acgttctcgc cggcatgaag gtctcgcatc aggtgcagca ggatggcgga ctctgattcc
     3301 cagctcgatc tcaagaagcg ggcgcgacgg cgcctggtcg gatcggcggc gctggccctg
     3361 acggcggcca tcgtgctgcc catggtcatg gaccacgaac cgcgaccgcc ctcccaggac
     3421 gtgcagatcc gcattccaag ccaggaaggc agcaatttca cgtcgcgggt gatctcgggc
     3481 aaggggcctg cgccgactca gcctgttgcc ggagcggccg atgcggctgt cgatgacggc
     3541 gtcgcgctgc cccgcgacag gacagtgccc aagacggtgc aaccggaggc cggggcacct
     3601 gccggccgtg tggatccggt caggccacag gcggcggtgg agtctccgcc cgacgtgaag
     3661 cctgacgtcc gtccggtgcc caagccggac gcgaaacctg agcccaggaa gccagaaccg
     3721 aagccggagc ccaagaagcc ggaacctaaa ccggaaccca agccggagcc ggaacccaag
     3781 cccaagcctc ccaccgacgc ggagcgggcc aaggccctgc tcaacggcga ggcgccgacg
     3841 gccggggcta acggccccca tgtggtgctg ctgggcgcct accgcgaagc ggcgaatgtg
     3901 acggccctcc gggcacggct caaggccgag ggattcgcga cctacaccga gccgctgggc
     3961 gacaagatcc gggtgcgggc cgggcccttc cccaacaagg cggcggcgga caaggcggcc
     4021 gcgcgcatct cgaagatgat gggcgtgtcc gcgtcagtgg cggtcaagcc ctaggtcgcg
     4081 caggttcttg aacggattcg actacgccat gctcgggctc ctggggctct ccgcgctgct
     4141 tgggttctgg cgcgggctga tctcggaagt tgtcgcgctg ggtgcctggg tgctggcttt
     4201 tctggtggca aaggccttcc tgcccgacgt gacgccgtgg ctcgccgcgt gggtgcgtga
     4261 gccggtgctg caggcgcccg tggccttcgt cgtgatcttc gtgggcgtac tggtgggggt
     4321 ggccctgcta cgctggctgc tgcgcgagtt gatccaggca gccgggctgg gactggcgga
     4381 ccgtttcctg ggggcctgtt tcggcctcgt gcggggcggc gtgatcgtat ttgcagttgc
     4441 ggtgctggtc ggactggctg gcctcgcccg ggaagcgtgg tggcgcgaag cgagtctgtc
     4501 cgcgccgctg gaaacggcgg tgctggctac ccggccgtgg ctgcccgacc ccctgacaaa
     4561 gagacttcga ttcagataag gtggtttgaa atgtgtggga ttctgggcgt agtcgcgact
     4621 acacgcgtca atcagctcct gtatgacggc ctccaggtgc tccagcaccg gggccaggac
     4681 gcggcgggca tcgccacggc cgaagggggg cgctttcaca tgcacaagtc ctcgggcctg
     4741 gtgcgcgatg tgttccgcac gcgcaacatg cgggatctgc ccggcaactg gggcatcgcc
     4801 cacgtgcgct atccgaccgc cggttccgcg tcgtccgcgg cggaggcgca gccgttctac
     4861 gtgaactcgc cgttcggcat catgctggcc cacaacggca acctgaccaa cgccgaagat
     4921 ctgaagcggg agatgtttct ctccgacctg cgccacatca acaccaactc cgattcggag
     4981 gtgctgctca atgtgctggc gcacgaactc caggccgccg cgagcagctt ccgtctcgat
     5041 caggacacgg ttttcaaggc cgtggccggc gtgcatcggc gctgccgcgg agcctacgcc
     5101 acggtggcca tgatctccgg tacgggcctc ctggccttcc gcgatcccta tggcatccgc
     5161 ccgctggtga tcggccggaa cgatacgccc gccggcaccg agtggatggt ggcatccgag
     5221 agcgtggccc tggatgtgct cggtttccgc ctggtgcggg acgtggcgcc cggtgaggcg
     5281 atcctcatcg atgcggaggg tggattccac agccgtcagt gtgcgacgca cacggtgcac
     5341 gcgccgtgca tgttcgagtt cgtctacctg gcgcggcctg attcactgat cgacaacatc
     5401 tcggtgtacg acgcccgcat caagatgggt gaattcctgg cgcgcaagct gcgttccacg
     5461 atgccgcacc tggaaatcga cgtggtcatc ccgatccccg attccagtcg tccatcggca
     5521 atggagatgg cccatcatct caacgtcccg tatcgggaag gcttcgtcaa gaatcgctac
     5581 atcggccgca ccttcatcat gcccggcctg tccatccgtc ggaagtccgt acgccagaag
     5641 ctcaatgcga tccatcagga attccgcggc aagaaggtgc tgctggtgga cgattccatc
     5701 gtgcgcggga ccaccagccg cgagatcgtg cagatggccc gcgatgccgg cgcaacgcag
     5761 gtctacatgg cgtcggcggc gcctccggtg cgctttgcca acgtgtacgg catcgacatg
     5821 ccgacgcgtc atgagctcat cgccagcaat cggaccgagg acgagatctg tgccgagatt
     5881 ggcgctgacg cgttgatcta ccaggatctt gaggatctga aggcggcggt gcgtgcgctc
     5941 aatccggtca tctcggtgtt cgagacctcg tgcttcgacg gctgttacgt gaccggtgat
     6001 gtgaccgccg aatacctcga cggcgtcgag acgcagcgga gtgaggcgcc gtccgatgcg
     6061 tccgagggcg cagcgcagat ggacctgaat ctcgtcaccg ggcacgaggg ctgaaggatc
     6121 atgaacccac ttcatccaga aaccctggcc gtccgggctg gcacggcccg cagccagttc
     6181 aatgagcatt cggaagcgct ctatctgact tccagctttg tcttcgacag tgccgcccag
     6241 gcagcggcgc ggttttccgg cgaagaggaa ggcaacgtct acgcccggtt caccaacccg
     6301 acggtcaccg ccatgcaaca gcgtctggcg gccctggagg gtgcggaatc ctgcgtggcc
     6361 accgcgtctg gcatggcggc gatcctggcg acggtgatgg cgctgatgca ggcaggcgac
     6421 catgtggtgg cttcccgggg gctgttcggg gcgacccagc agctgttcgg caacatcctg
     6481 tccaagttcg gtatcgagac gagcttcgtg ccggccaccg atctggcggc ctaccgggcg
     6541 gcggtgacgc cccgtacgaa gctgttcttc gtcgaatccc cgtccaatcc gctcaccgaa
     6601 ctggtggaca tcggcgccgt ggccgccatc gcccatgagg ccggggcgct gctggcggtg
     6661 gataactgct tctgttcacc ggccctgcag caacccctgg ccctgggggc cgatctggtc
     6721 atccattccg cgaccaagta tctggacggg caggggcggg tgctgggcgg tgcggtctgc
     6781 ggtgcggcac accacgtgga tgaggtcttc aagttcctgc gcacggctgg tccgtgcatc
     6841 tcgcccttca atgcctgggt gatcctcaag ggcctggaga ccctgtccat ccgcatggcg
     6901 g
//
LOCUS       DAOQPA010000151         6890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6249_length_6890_cov_18.643745, whole genome shotgun sequence.
ACCESSION   DAOQPA010000151 DAOQPA010000000
VERSION     DAOQPA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6249_length_6890_cov_18.643745"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKX01_15335"
     CDS             <1..584
                     /locus_tag="PKX01_15335"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMZ77557.1"
                     /translation="GLAVLYGNIAEKGCIVKTAGVDESIWQFTGRARVFESQEDAVAG
                     ILGDQIVAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKVCALLTDGRFSGGTSG
                     LSIGHASPEAACGGAIGLVEEGDTIEIDIPHRRIHLAVADDELARRRAAQDARGWKPA
                     NRVRHVSAALQAYAALTTSADTGAVRDVTQIQR"
     gene            755..1747
                     /locus_tag="PKX01_15340"
     CDS             755..1747
                     /locus_tag="PKX01_15340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ77558.1"
                     /translation="MPSSFLPASSGPLDTSSHTTPCQIEVEPNRRRWGALFARVPAPA
                     LTQSIAYGDAKAAVSGWEVERLVFHVGGWPVAICQVLVRRVWGLPVAARINRGPLFLA
                     AEPAVTEIRAVYGALRRRWRFGRRGVVFLAPGLADGALHRQCLQEAGFRPRGVAGWRS
                     ALLDLRQPAAELRRRLASNWRNHLNGAERNGLSFELERTPEALAWMLEHHADHMREKG
                     FAGTSVDFLQALARRAPDDVFVARVRQAGTPLAGMILCRFGNSAEYFVGWYGTEARRA
                     KAGHFLVWHAALAMAAVGCARFDLGGYSSSDGYGRFKQDMRGTEYTLADEWIAF"
     gene            complement(1799..2290)
                     /locus_tag="PKX01_15345"
     CDS             complement(1799..2290)
                     /locus_tag="PKX01_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ77559.1"
                     /translation="MNTVSIQPVDFGDAVRAEAYLDLLDHYMRGPTGSGTPLSPALRT
                     RLTAELRQRPTVLCFVAYLDDTPAGLLTCVEGFSTFAGRPLLNIHDIVVHERFRRRGL
                     ACALLEKAEDVARTRGCCKLTLEVLEGNAGARQAYLEFGFRAYELDPAMGRALFMEKR
                     LEG"
     gene            complement(2356..3312)
                     /locus_tag="PKX01_15350"
     CDS             complement(2356..3312)
                     /locus_tag="PKX01_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77560.1"
                     /translation="MTPVEAREWKTYSFQPQEAMDWKAAGFAPLVARTWSDKGFDPDE
                     ARAWVESTRQNRTLMAELDQTDPAAWKREGFSPADRLAWWEAGFVFEDAVLLSRAGMS
                     PAEAAWHGQEKLKELKEKSPTNVAGAAAPPVTGSPDSAPAAGLTLSLPDPARLWALTW
                     PYLKIAMASMVGMMVALIVFVYVRNRRLNRVHRSALEAAPDSELPPSPQPPAAAAAAE
                     SEGVNTTKSKHRRPARKFTLTRGQPTCVHCQSTHVRPSRMNPHRFLGVNFTEYYRCRN
                     CGRHFALVTYTPILLIAGSILLAVTMVTASVIYGLSLIPGSP"
     gene            3647..3943
                     /locus_tag="PKX01_15355"
     CDS             3647..3943
                     /locus_tag="PKX01_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ77561.1"
                     /translation="MSKWSRRAEEVRRLEDLPNVGPAIARDLRSIGIPDPAALRDADP
                     WALFACLNARDGVRHDPCVLDTFIAVTRFMNGDPARPWWAYTAERKRREAMDAG"
     gene            complement(3952..4377)
                     /locus_tag="PKX01_15360"
     CDS             complement(3952..4377)
                     /locus_tag="PKX01_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ77562.1"
                     /translation="MARVLIDLPEHFVFTTELPIYIGHINYGHHLDNAALLSLVSEAR
                     VRFFGSLGYTELDVEGVGIVVADAAVQYKSEAFHGETLVFAMTPAEFNKYGCDLVWRV
                     TDKASGREVARGKSGILFFDYQARRPVAVPDGFRIKTAP"
     gene            4588..5277
                     /locus_tag="PKX01_15365"
     CDS             4588..5277
                     /locus_tag="PKX01_15365"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ77563.1"
                     /translation="MGETPQRGLWFALGMSVAAHLLVLAFGRGPQPELAPPRLLDARL
                     VSESTPERPHEPPPRHPPAETRPVHAAPAPHPQRQEALPVPSRMVSDAVSSRPTPAIA
                     TSDSPLPSRSEAPAVAAPPAAAAVSSHAAYTPPSSSASYLDNPKPGYPMIARRRGLQG
                     RVLLDVRVSAEGLALFVKVKESSGHDVLDEAAVNAVAHWRFAPARRGGEAVEGTASVP
                     VQFRLNGTEPE"
     gene            5306..5923
                     /locus_tag="PKX01_15370"
     CDS             5306..5923
                     /locus_tag="PKX01_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMZ77564.1"
                     /translation="MALSHWLAFFLTTLVVALSPGPGAVLSMSTGLRDGVRAALMAIA
                     GLQTALLVQLGLVALGMGAVLAASDTAFMVIKFAGAAYLILLGLRAWRDTGPPNVADR
                     GGAQRGRHYRQGILVNLGNPKAILFIAALVPQFVDPAQPRPPQFMIMALTLCTVDTVV
                     MSGYAMLAARCRAWMTDARAVRTRNRFFGTLFVVAGMALALATRA"
     gene            complement(5928..6740)
                     /locus_tag="PKX01_15375"
     CDS             complement(5928..6740)
                     /locus_tag="PKX01_15375"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ77565.1"
                     /translation="MNGDEDALVRAVLASGVILPVPAPGFLRFQAAAADDTAGPKALA
                     AAVIDDPALTGALLRVASSPVFRPRSAPRNVLEAVSLLGRTRSLATVASTALRSRCDG
                     LDASAVDSLWRAGARAADAAFWACRASSCKSLADLTYLAALLQDVGTVVVLRRTPELA
                     GLLRCAEPDLEAGIHQLDTASGTCHAAAGYLVARNWKLPTAVCEALRLHHDLRATLRA
                     EPDVRHLGLLLAAGRRMRDGPDTPAWPEWSDPVAAELGLDSPTLDALAAGLG"
BASE COUNT         1031 a         2192 c         2515 g         1152 t
ORIGIN      
        1 gcggcctcgc cgtgctctac ggcaacatcg ccgagaaggg ctgcatcgtg aagaccgccg
       61 gcgtggatga gtccatctgg caattcaccg gccgcgcccg cgtgttcgaa agccaggaag
      121 acgccgtggc cggtatcctc ggcgaccaga tcgttgccgg cgacgtggta gtgatccgct
      181 acgaagggcc caagggcggg ccgggcatgc aggaaatgct ttacccgacc agctatctga
      241 agtccaaggg cctcggcaag gtgtgcgcgc tgctcaccga cgggcgcttc tccggcggca
      301 cctcgggcct gtccattggc cacgcctcgc cggaagccgc ctgtggcggc gccatcggtc
      361 tggtggagga aggcgacacc atcgagatcg acatccctca ccggcgcatc cacctggccg
      421 tggccgacga cgaactggcc cgccggcgcg ccgcccagga tgcccgtggc tggaagccgg
      481 ccaaccgcgt tcgccacgtg tcggcggcgc tgcaggctta tgcggcgctg accacctcgg
      541 cagacaccgg cgcggtgcgt gacgtgaccc agatccagcg ctgagccgca tcgctccctg
      601 ttgcgggggg tgtcttccgg accgctgcag accgcgaatt agtacgcggt ctgcagcggt
      661 ttttcattgt gcagacaagg gcttgtgacc gatgtccggg agtcgacgga ggcggcgggg
      721 agaatcggag cgttccggac tttccccgac cgccatgccc agcagcttct tgccggcttc
      781 atccggcccg ctcgacacct catcacacac caccccatgc cagatcgagg tggagcccaa
      841 tcgccgccgc tggggtgccc tgttcgcccg cgtgcccgca ccggcgctca cccagtccat
      901 tgcttatggg gatgccaagg cggccgtgag cggatgggag gtggagcgcc tggtcttcca
      961 cgtgggaggc tggccggtgg cgatctgcca ggtgctggtg cgccgtgtgt ggggcctgcc
     1021 ggtggcggcg cggatcaacc gcggaccctt gttcctggcg gcggagcccg cggtgacgga
     1081 gatccgcgcc gtgtatgggg ccttgcgccg gcgctggcgc ttcggccggc gcggcgtggt
     1141 gtttctggcg ccagggctgg ccgatggggc gctgcatcgg caatgtctgc aggaggcggg
     1201 cttccggccg cggggtgtgg cgggatggcg ctctgcactc ctcgatctgc gccagcctgc
     1261 ggccgaactg cgtcgccgcc tggcgtcgaa ctggcgcaat catctgaacg gggcggagcg
     1321 gaacgggctg agtttcgaac tggagcggac acccgaggcg ctggcgtgga tgctcgaaca
     1381 tcacgcggac cacatgcggg agaagggctt tgcgggcacc tcggtggatt tcctgcaggc
     1441 gcttgcccgt cgtgcgccgg acgatgtctt cgtggcgcgg gtccggcagg cgggcacgcc
     1501 gttggccggg atgatcctgt gccgattcgg gaacagcgct gagtacttcg taggttggta
     1561 cggcacagag gcacggcgcg cgaaggcggg gcacttcctc gtctggcacg cggcgctggc
     1621 catggctgcc gtgggatgcg ctcgcttcga tctgggcggc tattcgtcgt ctgacggcta
     1681 tggccgtttc aagcaggaca tgcgcgggac ggaatacacg ctggcggacg aatggatcgc
     1741 cttttgattc cgggtcgtcc gcccgggtcg gcggcgcgcg gggggcacga ccggtgcttc
     1801 agccctccag ccgcttttcc atgaacaggg cgcggcccat cgccggatcg agctcgtagg
     1861 cgcggaagcc gaattccaga tacgcctgtc gcgcgccggc attgccttcc agcacttcga
     1921 gggtgagctt gcagcaaccg cgtgtgcggg cgacgtcttc ggccttttcc aacagggcac
     1981 aggcgaggcc gcgacgtcgg aaacgttcgt ggacgacgat gtcgtgaatg ttgagcaggg
     2041 gacgtccggc gaaggtggaa aatccttcca cgcaggtcag caggcccgcc ggggtgtcgt
     2101 ccagataggc cacgaagcac agcaccgtcg ggcgctggcg cagttcggcc gtcaggcgcg
     2161 tccgcagggc cgggctcagg ggcgtgccac tgccggtcgg cccgcgcatg tagtggtcga
     2221 gaaggtccag gtaggcttcg gcgcggacgg cgtcgccgaa gtccaccggc tgaatgctga
     2281 cggtgttcat gggtcacccg agacagggga agggcgcatc ttgccacgcc ccgccctcgg
     2341 ccgggatggg ctccgtcagg gcgagcctgg aatcagtgac agcccgtaga tgacggaggc
     2401 ggtgaccatg gtgaccgcca gcaggatgct gcccgcgatc agcaggatgg gcgtgtaggt
     2461 gacgagcgcg aagtgccgtc cgcaattccg gcaccggtag tactcggtga aattgacgcc
     2521 gaggaagcgg tggggattca tccgcgacgg ccgcacatgg gtgctctgac agtggacgca
     2581 ggtgggctgc ccgcgcgtga gcgtgaactt gcgggccgga cgacggtgtt tgctcttcgt
     2641 cgtattgacg ccttcggatt cggccgccgc ggcggcggca gggggctgcg gcgagggcgg
     2701 cagctcgctg tccggagccg cctcgagtgc gctacgatgg accctgttga ggcgccggtt
     2761 gcggacgtac acgaagacga tcagcgccac catcatgcca accatggagg ccatggcgat
     2821 cttgaggtaa ggccaggtca gggcccacag tcgggccgga tcgggaagac tcagggtcag
     2881 accggctgca ggggcgctgt cgggcgagcc ggtcacaggg ggcgcagcgg cgccggcgac
     2941 gttggtcggg ctcttttcct tcagttcctt gagtttctcc tgaccgtgcc acgcggcttc
     3001 ggcgggcgac atgcccgccc gtgacagcag gacggcatcc tcgaaaacga agccggcctc
     3061 ccaccaggcg aggcggtcgg ctggcgaaaa cccctcgcgc ttccacgcgg cggggtcggt
     3121 ctgatcgagt tccgccatca gggtgcggtt ctggcgggtg gactcgaccc aggcgcgcgc
     3181 ctcgtcgggg tcgaagccct tgtcggacca ggtgcgggcc accaatggtg cgaatccggc
     3241 ggccttccag tccattgctt cctggggctg gaacgaatag gtcttccact cccgggcctc
     3301 gacaggcgtc atgcctgctt cttcccagcg tgtgacatgg ggggcgctgg gcgtcgcggc
     3361 ctccgcggag gctgcggcct tttgcgcagg ggccgtgggg gcgggtcgtg gaccggggtc
     3421 ggcaaccgcg attccacaga cgagaagcgc ggcggacagg aatgggaggg tctgtttcat
     3481 ggtgcgaaac attaatgcac tccggggggc gcgcaccatg aagacttcgc gtgccccgtg
     3541 aaataaatcg taaggctatg cgaatatggc cctggcaagt gcggggcgcc tcacttcgcc
     3601 acattcccga aataattcaa gcctgttgcg ctgcgaaggg gcaaatatga gcaaatggtc
     3661 ccggcgggcc gaggaggtcc gccgtctgga agatcttccc aatgtcggtc cggctatcgc
     3721 gcgggacctg cgcagcatcg ggatcccgga tccagcggcc ttgcgcgacg ccgacccgtg
     3781 ggcactcttt gcgtgcctca acgcccgcga tggtgtccgc cacgacccct gcgtactgga
     3841 caccttcatc gcggtcacgc gcttcatgaa cggcgacccg gcccggccgt ggtgggccta
     3901 cacggccgaa cgcaagcgcc gtgaggccat ggatgcaggc tgagcacggc ttcagggggc
     3961 cgtcttgatc cggaagccgt ccggcacggc gaccgggcgg cgggcctggt agtcgaagaa
     4021 gaggatgccc gacttgccgc gggccacttc ccggccgctg gccttgtccg taacgcgcca
     4081 caccaggtcg cagccgtatt tgttgaactc cgccggcgtc atcgcgaaca ccagggtttc
     4141 cccgtggaag gcctcggatt tgtactggac ggcggcgtcc gccaccacga tgccgacacc
     4201 ttccacatcg agttccgtgt agccgagcga tccgaagaag cgcacccggg cctcggagac
     4261 gagggacagg agcgccgcgt tgtccaggtg atggccgtag ttgatgtggc cgatatagat
     4321 gggcagttcg gtggtgaaga cgaagtgttc cgggaggtcg atgagaacgc gggccatgag
     4381 tgatccttgc tgtgggcggg tgcgacaaaa gcgcgcaggg tctgcggcct tgatccagat
     4441 cgtccaccgt gtcggagcga tccggtagga tgcgcagtgt aagggggccg gcccagagga
     4501 gtcatgggcc ggcagttttt ttgtagcgag gggcatgccc gaatgaatgc cggagccgct
     4561 tgtctgtccg ggcttgggaa aagagaagtg ggcgagacgc cgcagcgcgg actgtggttc
     4621 gcactaggga tgtccgtcgc ggcgcatctc ctggtcctgg cattcggccg cgggccgcag
     4681 cccgagctgg cgccgccacg cctgctggac gcgcggctgg tgagcgagtc cacacccgag
     4741 cgtccccatg agccgccacc gcggcatccg cccgccgaga cgcgcccggt ccatgctgcg
     4801 cctgcgccgc atccgcaacg tcaggaggcg ctgccggtgc ccagccggat ggtctccgac
     4861 gccgtgtcgt cgcggccgac gccggccatc gcgacgtccg actcgccgct accgtcacgc
     4921 agcgaagcac cggccgtggc tgcgccgccg gccgctgcgg ccgtgtcgtc ccacgccgcc
     4981 tacacgccgc cgagttcgtc ggcttcctat ctggacaatc ccaagcccgg ctatccgatg
     5041 atcgcccgtc gccgtggtct gcaggggcgc gtgctgctgg acgtgcgggt ctctgccgag
     5101 ggcctggccc tgttcgtgaa ggtgaaggag agttccggcc acgacgtgct cgacgaggcc
     5161 gcggtcaacg ccgtggcgca ctggcggttt gcgccggcac ggcggggtgg cgaggccgtg
     5221 gagggcacgg caagcgtgcc ggtgcagttc cgcctcaacg ggacggagcc ggagtgaggc
     5281 gcccactacg cgccgggcgc ctgtgatggc cctgtcccat tggcttgcct ttttcctgac
     5341 gacactggtg gtcgccctct cgccggggcc gggtgctgtg ctgagcatga gtacaggcct
     5401 gcgtgacggt gtgcgggcgg ccctgatggc catcgccggt ctgcagaccg cgctgcttgt
     5461 gcaactgggc ctggtggcgc ttggcatggg ggccgtgctg gctgcttcgg acaccgcttt
     5521 tatggtgatc aaattcgccg gcgccgcgta cctgatcctt ctggggctgc gtgcctggcg
     5581 cgacacgggg cccccgaacg tcgccgatcg gggcggagcg cagcggggcc gccattatcg
     5641 ccagggaatc ctggtcaatc tgggcaaccc gaaggcgatc ctcttcatcg cggcgctggt
     5701 gccccagttc gtcgatccgg cgcagccgcg tcctccccag ttcatgatca tggcgctgac
     5761 cttgtgcacc gtcgatacgg tggtgatgtc cggctacgcc atgctggcgg ccaggtgtcg
     5821 tgcatggatg accgacgccc gtgccgtgcg gacccgcaat cgcttcttcg gtacgctgtt
     5881 tgtcgtggcg gggatggccc tggcgcttgc gacccgggcc tgacccctca gccaaggccc
     5941 gccgcaaggg cgtcaagcgt cgggctgtcg agcccgagct ccgctgcgac cgggtcggac
     6001 cactccggcc aggccggtgt gtccgggccg tcgcgcatgc gtcggccggc agccagcagg
     6061 aggccaaggt ggcgaacgtc gggctcggcg cgcagcgtgg cccgcaggtc gtggtggagt
     6121 cggagggcct cgcagaccgc tgtgggcagc ttccagttgc gggccacgag atagcccgcc
     6181 gcggcatggc aggtgccgct ggccgtgtcg agctggtgga tgcctgcctc caggtccggt
     6241 tcggcgcagc ggagcagccc ggcgagctcc ggtgtccgcc gcagcacgac gacggtgccg
     6301 acgtcctgca ggagtgcggc gaggtaggtc agatcggcga gtgacttgca cgatgacgcc
     6361 cggcacgccc agaaggcggc atccgctgcg cgtgcgccgg cgcgccagag cgagtcgacg
     6421 gcggaagcgt cgagtccgtc gcagcgggac ctgagggccg tgctggcgac ggtggccagg
     6481 ctgcgcgtgc gtcccagcag cgagacggcc tcgagcacgt tccgcggggc gctgcggggg
     6541 cggaagaccg gtgaactggc gacccgcagg agtgcgccgg tgagtgccgg gtcgtcaatg
     6601 accgccgcag ccagtgcctt tggccccgcg gtatcgtcgg ccgcagcggc ctgaaaacgc
     6661 agaaagccgg gggcggggac cggcagtatc acgccgctgg cgagtacggc gcgaaccagt
     6721 gcgtcctcat caccgttcat gcgggggcgt ctccattctg aggggactcc atggtgcccg
     6781 atttattttc ctgaagagtg tttgacattc cccgaaacgg tatctacaat gcggggcttc
     6841 tcgcttggag gggttcccga gcggtcaaag ggatcagact gtaaatctga
//
LOCUS       DAOQPA010000152         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6252_length_6889_cov_17.037606, whole genome shotgun sequence.
ACCESSION   DAOQPA010000152 DAOQPA010000000
VERSION     DAOQPA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6252_length_6889_cov_17.037606"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKX01_15380"
     CDS             complement(<1..734)
                     /locus_tag="PKX01_15380"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMZ77566.1"
                     /translation="MNATDKFIASSAHVDEAAIAPLPNSRKIYVEGSRADLRVPMREI
                     RQADTPTAMGGEQNPPIFVYDCSGPYTDPAARIDIRSGLPALRQAWIEGRGDTEVLPD
                     LSSEYGRVRAADTRLDELRFPGLHRAPRRARAGANVTQMHYARRGVVTPEMEFVAIRE
                     NLRRTEYVESLRASGPLGERMAKLLTRQHPGQSYGAAIPATITPEFVRDEIARGRAIL
                     PANVNHPETEPMIIGRNFLVKINANIG"
     regulatory      complement(772..871)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1037..2512
                     /locus_tag="PKX01_15385"
     CDS             1037..2512
                     /locus_tag="PKX01_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HMZ77567.1"
                     /translation="MRLRMRPLVVLLALAGASQALAADWQTVAADKKRKIELDRASIL
                     QSDPGTKVAWGRIVLAEAEAEAAGYATVKALNRYDCRNQTFATIKRVYLDGDAAVLKD
                     EKVKVEKDMPITAGSVDEKLWKEVCQPGGTKGLNQVAQEASKAAQAAAAKPDLRAADY
                     RPAKGAEKPAVRQVNEGKGEEPPATPIKKNFIDKPAPPPKAEVAEKPAEKTAEKAPAP
                     RVEAAPPEVRRVPAYVPPRPRVVRMPAVAVEAGAGHGPDAAGTPLAHHEIHWSYDGEG
                     GPPNWGVLKPEWGLCSTGERQSPIDIREGIKVELEPIRFDYKPSYFRVVDNGHTIQVN
                     LGPGNTLSVMGRQYDLVQFHFHRPSEERINGKGFDMVAHLVHKDLDGRLAVVAVLLER
                     GSAHPLIQTLWNNLPLEKNVEYGPEGVSIDLNQLLPDNRSYYTYMGSLTTPPCSEGVL
                     WMVLKQPAQMSADQIGIFSRLYPMNARPVQSIKGRLIKESR"
     gene            complement(2496..3980)
                     /locus_tag="PKX01_15390"
     CDS             complement(2496..3980)
                     /locus_tag="PKX01_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HMZ77568.1"
                     /translation="MLRITLSPDSPTPLTDQIVAALCARVDDRVLRPGMRLPPIRQFS
                     ADHGVSRFTVVEAYDRLVALGYVQSRRGSGFYVAGRPAPARTGETDTGVERAIDAAWL
                     MREMASGGPERLLAGTGNMPVAWLDEAGVRRALRSLARRPDVRPASYGTAQGYAPLRA
                     QLQIQLAGHGIRATPEQIVLTYGAHQALDILCKYFLKPGDCVLVDDPGYWNLFGNLRL
                     YGVALLGVPRTPEGPDTAALEALLAHHKPRLFFTQSVFHNPTSGCLSPACAFRVLQLA
                     ARHDFMIVEDDIVGDIAGPAATRLASLDQLERVIFVGSFSKTLSGNLRVGFLACRPDL
                     ARLLTDVKIVSMMSSAEFSEQLVHQMLTDGHYRKLLERLRDRIADATGRSLRQLERLG
                     LHPDPEPAGGTFLWVPLPGVEDTAELARVALQDDILLAPGSVFRPQNQPSPYLRVHVA
                     HGDDPRLLRFLARALDDAHKKRASPEGRPGEALTNAADRLSGIP"
     gene            4107..4580
                     /locus_tag="PKX01_15395"
     CDS             4107..4580
                     /locus_tag="PKX01_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77569.1"
                     /translation="MVRGASIRGLIALIGLLPLLALAGGARAQVWWPERQWVFVADRD
                     GGSVQVLALRSGLTPVAVLRQRDRSAVRGIAVDPRAVRAWVLGPRELDLHDAANGRLI
                     AHYAAPAGMLLEGFEPADGRSLRVRGGGALFEPRPGCGCLARVDAVLTGREMRAP"
     gene            4681..6735
                     /locus_tag="PKX01_15400"
     CDS             4681..6735
                     /locus_tag="PKX01_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ77570.1"
                     /translation="MTQHGSAPWPLGRRRTFAAFVLTGGVLASRAWSGSDVDALAELP
                     LEDLLQVEVSTVSRHAQRLAEAAAAVHVITAEEIRHAGVRTLPDALRLAPGVDVAQIG
                     ADRWAVSLRGHTDYLANKLMVLVDGRSVYSPNFSGVLWNTVQVPLENIERIEVIGGPG
                     GALWGANAVNGIINIITRPAQATQGTLVNAGHGSAEGSFGLARQGGRFDEDTFYSAYV
                     SATRGAGLAAAGREDRPDGNVDSGAGLRIDARRGRDAWMLNSAVYRSRVGGTGRVPDP
                     LAAGAGFGRNVALADDFSGVDVHGRLSRQLDDGGEVQASVAWSHADLDSGLLARERQD
                     IVDMEVQHRLPPRSGHRLTWGAGLRGFMDDARDGAVTHLTRTRETLVVASLFAQDEIA
                     VTGTVRLTLGARLDQQKYTGAGLQPSARLLWQPAPGQTAWAAVSQALRLPSRGERTTD
                     YRLGVLPPASPLNPAPLPALLEVRGSGGYGAEHLTAWEAGWRGQPGAGLVLDASVFLH
                     RYRGLRTASGGPPEMAASASYMVIPALFSNLGEQTLRGVEAAAHWRPVEGWRLRAAAT
                     YRVTSPFEAGATMFQDLFASQAPRRAANLLSGWRLGSRTDLDLWLVRVGPRNVPGQAA
                     LPASNRMDLRLARRLDRRVEVALVGQNLLDDRHGEYVSFVPGFAPVEVRRGIYGQIRV
                     SD"
BASE COUNT         1050 a         2115 c         2607 g         1117 t
ORIGIN      
        1 ccgatgttgg cgttgatctt caccaggaaa ttgcggccga tgatcatcgg ctcggtctcc
       61 ggatggttga cgttggccgg caggatggcg cggccgcggg cgatttcgtc gcgcacgaac
      121 tccggcgtga tcgtcgcggg gatggcggcg ccgtagctct ggccggggtg ttggcgggtc
      181 aggagtttgg ccatgcgttc gcccaggggg ccgctggcgc gcagggactc cacgtattcg
      241 gtgcggcgca ggttctcgcg gatcgccacg aactccatct cgggcgtgac gacgccgcgg
      301 cgggcgtagt gcatctgggt cacgttggcg ccggcgcggg cgcgccgcgg tgcgcggtgc
      361 agaccgggga aacgcagctc atcgaggcgg gtgtcggcgg cgcggacgcg gccgtattcg
      421 gaggacaggt cgggcagcac ctcggtgtcg ccgcgcccct cgatccaggc ctggcgcagg
      481 gcgggcaggc cggagcggat gtcaatgcgt gcggccgggt cggtgtaggg gccggagcag
      541 tcatagacga agatcggcgg attctgttcg ccgcccatgg cggtgggcgt gtcggcctgg
      601 cggatctcgc gcatgggcac gcgcagatcg gcgcgggagc cttccacgta gatcttgcgg
      661 gagttgggca ggggcgcgat ggccgcttcg tccacgtggg cggacgaagc aatgaatttg
      721 tcggtggcgt tcatgaaggc tcccagggga gggccgcggg cgggggaaac gacggcggga
      781 gccacgtttc cctacgccgg tacgacccgg atcaggttcg gagggtaagt ctcagccgcg
      841 cgcatgggca gcggcacccc taacgtatgg cccggaaagt actggaagcg gccggtcatt
      901 gcaagatttt ccggaaaatc aaggctccgc gccattcccc ggcgctgcgg agcgtttcta
      961 aagctttgcg gcaccccgcc gaaataggag gcatccggtc cattgcggcc cctgcatccc
     1021 ttttcggaga atcaccatgc gactgcgcat gcgtcccctc gtcgtcctgc tggccctggc
     1081 cggcgcgtcc caggccctgg ccgccgactg gcagaccgtg gctgcggaca agaagcgcaa
     1141 gatcgaactc gaccgggcga gcatcctgca atccgatccg ggcaccaagg tggcctgggg
     1201 gcgcatcgtg ctggccgagg ccgaggccga ggcggctggc tacgccaccg tgaaggccct
     1261 caaccgctac gactgccgca accagacctt cgccaccatc aaacgcgtct atctggatgg
     1321 cgatgcagcc gtgctgaagg acgagaaggt caaggtcgag aaggacatgc ccatcaccgc
     1381 gggcagcgtg gacgagaagt tgtggaagga ggtctgccag cccggcggca ccaaggggct
     1441 caatcaggtt gcccaggagg cgtcgaaggc ggcccaggcc gcggccgcca agccggatct
     1501 gcgtgcagcc gactatcgtc ccgccaaagg tgccgaaaag cccgcggtgc gtcaggtgaa
     1561 tgaagggaag ggtgaagagc cgccggcgac gccgatcaag aagaatttca tcgacaagcc
     1621 ggcaccaccg cccaaggcgg aggtggccga aaaacctgcc gagaagaccg ccgagaaggc
     1681 gccggcaccc cgtgtcgaag cggctccgcc ggaagtgcgt cgcgtgccgg cctatgtgcc
     1741 gccgcggccg cgggtggtgc gcatgcccgc cgtggcggtg gaggcgggcg ccggccatgg
     1801 cccggatgcc gccggcacgc cgctcgccca tcacgagatc cactggagtt acgacggcga
     1861 aggcggcccg cccaactggg gcgtgctcaa gcccgaatgg gggctgtgca gcaccggcga
     1921 gcgccagtcg ccgatcgaca tccgtgaggg catcaaggtg gagctggagc cgatccgctt
     1981 cgactacaaa ccctcatatt tccgcgtggt ggacaacggc catacgatcc aggtcaacct
     2041 gggtccgggc aataccctct cggtgatggg gcgccagtac gatctggtgc agttccactt
     2101 tcaccggccg tcggaggagc gcatcaacgg caagggcttc gacatggttg cgcacctggt
     2161 ccacaaggac ctggacggcc gtctggccgt ggtggccgtg ctgctggagc ggggcagcgc
     2221 ccatccgctg atccagaccc tgtggaacaa cctgccgctg gagaagaatg tggagtacgg
     2281 cccggagggg gtgtccatcg atctcaacca gctcctgccg gacaaccgca gctattacac
     2341 ctacatgggt tccctcacca cgccgccctg cagcgagggc gtgctgtgga tggttctgaa
     2401 gcagccggcg cagatgtctg cggaccagat cggcatcttc tcgcgcctgt atccaatgaa
     2461 cgcccgcccg gtgcagtcga tcaaggggcg tctcatcaag gaatcccgct gagccggtcc
     2521 gccgcattcg tgagtgcctc tccggggcgt ccttcagggg acgccctttt tttgtgtgcg
     2581 tcgtccaggg cgcgggccag aaagcgcagc aggcgtgggt cgtcgccgtg ggccacgtgc
     2641 acgcgcagat agggggaggg ctggttctgg ggacggaaca cgctgcccgg cgcgaggagg
     2701 atgtcatcct gaagcgcgac gcgggcgagc tcggcggtgt cctccacgcc cggcaggggc
     2761 acccacagga aggtgccgcc ggcaggttcg gggtcgggat gcaggccgag gcgctccagc
     2821 tgccgcaggc tgcgtccggt ggcatcggca atccggtcgc gcagacgctc cagcagcttg
     2881 cggtagtggc cgtcggtgag catctggtgg acgagctgct cggagaactc cgccgaggac
     2941 atcatcgaaa cgatcttcac gtcggtgagg aggcgtgcca gatcgggccg gcaggccagg
     3001 aagccgaccc gcaggttgcc tgacagggtc ttggagaagc tgcccacgaa gatcacccgc
     3061 tcgagctggt cgaggctggc caggcgggtg gcagcggggc cggcaatgtc gccgacgatg
     3121 tcgtcctcga cgatcatgaa gtcgtggcgg gccgccagtt gcagtacgcg gaaggcacag
     3181 gccgggctga ggcagcccga cgtcgggttg tggaacaccg actgggtgaa gaacaggcgc
     3241 ggcttgtggt gggcgaggag ggcttccagc gccgccgtat ccgggccctc cggcgtgcgc
     3301 ggcacgccca gcagggcgac accgtagagg cgcaggttgc cgaacagatt ccagtagccg
     3361 ggatcgtcca ccagcacgca gtcgccgggt ttgaggaagt atttgcagag gatgtccagg
     3421 gcctgatggg cgccgtaggt gaggacgatc tgttccggtg tggcgcggat cccgtggccg
     3481 gcaagctgga tctgcagctg ggcgcgcagg ggcgcgtagc cctgggcggt tccgtagctg
     3541 gccgggcgca cgtcagggcg ccgcgcaaga ctgcgcaggg cgcgtcgtac gcccgcctcg
     3601 tcgagccacg ccacgggcat gttgccggtg ccggcgagca ggcgctccgg accgccggag
     3661 gccatctcgc gcatgagcca cgctgcatcg atggcgcgct ccacgccggt gtccgtctcg
     3721 ccggtgcggg cgggtgccgg ccggccggcc acatagaagc cggagccgcg acgggactgc
     3781 acgtaaccca gggctaccag gcggtcatag gcttccacca ccgtgaagcg ggagacgccg
     3841 tggtccgcgg aaaactggcg gatgggcggc aggcgcatgc caggccgcag gacgcgatca
     3901 tccacgcggg cgcagagggc cgcgacgatc tggtcggtga ggggggtcgg gctgtctggc
     3961 gacagggtga tgcgcagcat ggctttgacc tcggcgtacc ggtcgaaggg ccggtacggt
     4021 gtagtgagtt gtccgaaaat tgtatctgtg atgttgaaaa agtgtccgct attgtcaggc
     4081 tcattgagct tgaggggagt gcgggcatgg tacggggagc aagcatccgt gggctgatcg
     4141 ccctgatcgg tctgttgccg ctgctggcgc tggcgggcgg ggcgcgtgcg caggtgtggt
     4201 ggccggagcg gcaatgggtc ttcgtggcgg atcgggacgg cggatcggtc caggtgctgg
     4261 cgctgcgctc cggcctgacg cccgtggccg tgctgcgcca gcgcgaccgg agcgctgtgc
     4321 gtggcatcgc ggtcgatccg cgggccgtgc gggcctgggt gctggggcca cgggaactgg
     4381 atctgcatga cgcggccaac gggcggctga tcgcccacta tgcggcaccg gcaggcatgc
     4441 tgctggaagg cttcgagccg gccgacggac gttccctgcg ggtgcgtggt ggcggggccc
     4501 tgttcgaacc gcggccaggt tgcggatgtc tggcgcgggt agacgccgtg ctcaccggtc
     4561 gggagatgcg cgcgccctga tgcatctcaa gtccgtggat gaccggtacg ttaagatctg
     4621 tggtgcgtga acggatcaat ggctggggga cggaggcggg atgcggatcg ggggcggtag
     4681 gtgacacagc atggcagcgc gccgtggccc ctgggccggc gtcgcacatt tgccgcgttc
     4741 gtcctgactg gcggggttct ggcctcgcgc gcgtggagcg gttccgacgt cgatgccctg
     4801 gcggaactgc cccttgagga tctcctgcag gtggaggtga gcacggtcag ccggcacgcc
     4861 cagcgtcttg cggaggcggc cgctgcggtg catgtgatca ccgccgagga gatccgccac
     4921 gcgggggtgc gcaccctgcc ggatgccttg cggctggcgc ccggcgtgga tgtggcgcag
     4981 atcggtgcgg accgctgggc cgtgtcgctg cgggggcata cggactacct cgccaacaag
     5041 ctgatggtgc tggtggacgg gcgcagcgtc tatagcccca atttttccgg cgtgctgtgg
     5101 aacaccgtgc aggtgcccct cgagaacatc gagcgcatcg aggtgatcgg cggcccgggg
     5161 ggcgccctgt ggggggcgaa cgcggtcaat gggatcatca acatcatcac gcgaccagcg
     5221 caggccacgc agggcacgct ggtcaatgcc gggcatggct ctgcggaggg gagtttcgga
     5281 ctggcccggc agggtggccg gttcgacgag gacacgttct acagcgccta cgtgagtgcc
     5341 acgcgcggcg ccggactggc cgcggcgggg cgtgaggatc gaccggacgg caatgtcgac
     5401 agcggggccg ggctgcggat cgatgcccgt cgcgggcgcg acgcgtggat gctcaacagt
     5461 gccgtctacc gcagccgggt ggggggcacg gggcgggtgc cggatccgct ggcggctgga
     5521 gcgggctttg ggcgcaacgt ggctctggcg gacgattttt ccggagtgga tgtgcacggc
     5581 cggctctccc gtcagctgga cgacgggggc gaggtgcagg ccagcgtggc ctggtctcac
     5641 gccgatctgg actcgggcct gctggcccgc gaacgccagg acatcgtgga catggaggtc
     5701 cagcaccgcc tgccgccccg gtcggggcac cgcctgacct ggggtgcggg tctgcgcggc
     5761 ttcatggacg atgcgcggga tggggcggtg acccatctca cgcggacgcg ggagactctg
     5821 gtggtggcga gcctgtttgc ccaggatgag atcgccgtca cggggaccgt gcgcctgaca
     5881 ctcggcgcgc gtctcgatca gcagaagtac accggcgccg ggctgcagcc ttctgcgcgt
     5941 ctgctctggc aaccggcgcc cgggcagacc gcgtgggccg ccgtctccca ggccctgcgc
     6001 ctgccctccc gcggagaacg cacgaccgat taccggctcg gcgtgctgcc gccggccagt
     6061 cccctcaatc cggcgccgct gccggcactg ctggaggtgc gaggttccgg tgggtacggg
     6121 gcggaacacc tgacggcctg ggaggccggc tggcgaggtc agccgggggc cggtctggtg
     6181 ctcgatgcgt cggtctttct gcaccgctac cgtggcttgc gcacggcgtc cggcggaccg
     6241 cccgaaatgg cggcctccgc ttcctatatg gtgattcccg ccctgttttc caatctgggc
     6301 gagcagaccc tgcggggcgt cgaggcggca gcccattggc gcccggtgga aggctggcgt
     6361 ctgcgcgccg cggccaccta ccgcgtgacg tcgcccttcg aggcgggcgc gacgatgttt
     6421 caggatctgt ttgccagtca ggcgccgcgg cgagccgcca acctgttgtc cggctggcgg
     6481 ctgggcagcc ggaccgacct ggacctgtgg ctcgttcgcg tcggtccgcg gaacgtgccg
     6541 gggcaggcgg cgctgccggc cagcaaccgg atggacctgc gtctggcccg gcgcctggac
     6601 cgtcgtgtgg aagtggcgct ggtcgggcag aatctgctgg atgaccggca tggggagtat
     6661 gtctctttcg tgccgggctt tgcgccggtc gaagtccggc ggggtatata tggacagatc
     6721 cgcgtcagtg attgatttcc gtttctggca ccgttttggt gcggccgtct ttccagaccg
     6781 gtgcgcactg tccgaagcgg ggcacgaggc ggcatgtctt gttattaagg tgttgaattt
     6841 tattgaaaga gtaccttggc atggattctg cgacagactt tccattctt
//
LOCUS       DAOQPA010000153         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6580_length_6665_cov_18.155219, whole genome shotgun sequence.
ACCESSION   DAOQPA010000153 DAOQPA010000000
VERSION     DAOQPA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6580_length_6665_cov_18.155219"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..567
                     /locus_tag="PKX01_15405"
     CDS             154..567
                     /locus_tag="PKX01_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HMZ77571.1"
                     /translation="MTSSLYGPMLALIAWTGGVGMLAARRRVREIRRRRIPLATLARP
                     RDLAQALDDTQAMDNFNNLLQVPVLFHLCCLAFAQQASVPQAAAILAWVYVALRIGHS
                     LIQLTHNRVLPRFGVWTAGNLVLMGLWGVFAAGLV"
     gene            661..3000
                     /locus_tag="PKX01_15410"
     CDS             661..3000
                     /locus_tag="PKX01_15410"
                     /inference="COORDINATES: protein motif:HMM:NF018620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HMZ77572.1"
                     /translation="MRPLFALLIVFVTGCTAVAAHRLDERLGPRDPGRYDQAPAPVAP
                     APDYWTQVRPILDRRCVTCHACYDAPCQLNLSRYDGITRGASAETVYSSTRLTAAQPT
                     RLGFDALSTRAWRERGFFPVLNERSPTPEANREGSLLLRLLALKQAQPGPGSGALPDK
                     DFDFSLDRSQACAPLEGVGAYERSHPLWGMPFGLPALRSAEHDILSRWIEAGAPYTPP
                     PPAPPAVARRVAEWEALLNADAPGAQLAARYIYEHWYIGHLYFDEAPDIRFELVRSRT
                     APGAPIDPIATRRPYDDPGVARVYYRLRPNEATPVAKTYMPLVLDSARLTRLRKWFFD
                     TPATVDRLPGYAPETAANPFATFRALPVDSRYRFMLDDAKFTMMGFMKGPVCRGQVAL
                     NVIDDHFWVLFYAPEGEVSRNVQGLLDATQPNLRMPAEDSSSVGALAWLAYARAERNY
                     LNTKSAFMAGKTAVRPRLEDLWTGDGRNPNAALTVFRHFDSASVVRGLAGERPQTVLL
                     LGYPLFERMHYLLVAGFDVYGNVGHQLATRLYMDFLRMEGEQNFLTLLPLKDRQKVLD
                     HWYRGRSEPRIRQFADTSAYYPGETGVRFHGPDSLGELYRLAQRHVRPVREAGLDFAG
                     TGFSPAQGALLARLSAVKGRSASWMPELSLLALRKADGSVQPVTLIRNSAHSNVAQMF
                     NEDARRLPEEDTLLALPGFAGAYPNAIFQLDPAELPRFVNAVEGLTGEDDLLRLVQRF
                     GIRRTDSRFWPVSDALHDAWQRSSPREASILDYSRLENR"
     gene            3204..4433
                     /locus_tag="PKX01_15415"
     CDS             3204..4433
                     /locus_tag="PKX01_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ77573.1"
                     /translation="MESHSIEVAKHVLVTFGIILAIGSVSGFASRKLRMPDVAVFLLV
                     GMAIGPSALGWVDVKADSALNQIILIFGSSYILFDGGASLRLAVLKNVWVSIVLLSTL
                     GVLITAFITGYAAQWILGIPFIVALLLASAIASTDPATLVPVFRQVQIRERVAQTVMS
                     ESAFNDAVGAILTFAVLGLAMGQGEFSLSASLADLAVQAGVGLVAGLVLGWLAALFIA
                     HEKYAFLEEYAPVVSLMAVIGAYLGADGLHASGFMAVFVFGMVIGNKDVLGFEMGEEE
                     QHKLEEFVAITALIMRMFIFILLGSQVDFALMRAHLWEGLAVVAVFMFVARPATVFLC
                     ALPDRKARWSWQEMLFMCWTRETGVIPGALAGLLMGMGAPEAPLIASVTFIAILVTIL
                     LQATTTKWLAGRLGLLE"
     gene            complement(4529..5347)
                     /locus_tag="PKX01_15420"
     CDS             complement(4529..5347)
                     /locus_tag="PKX01_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77574.1"
                     /translation="MNTFFSRLMTLGVVTLAGLGVAGPAAANPFQIATDGSGLAGAQL
                     LSSFDLGGHGFVFQYMDPTSGLPMFQENGAFRIVASDGVSPIGTNDLTLTYALWGTAD
                     PLSGLISLIGGGFDLYSDSSFDFGTSTGIYGADDGTHVASFLVTGGSGDPMGTHLVSM
                     SAQLVAGSLLPGYLFTADHRDMSQLASVTLNASFVDNVATPDATAVEELVCEQAGLAA
                     LGCQGTAYANQIPYYFLVEDSASVTLNAVPVPGSLCLGALGLAMLGMHRRRMPG"
     gene            5613..5948
                     /locus_tag="PKX01_15425"
     CDS             5613..5948
                     /locus_tag="PKX01_15425"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ77575.1"
                     /translation="MTPPPDDLSPCQACGACCAQFRVSFYWSEALARTLPDAYVEQIN
                     PWMSCMAGTQGPAPRCAALHGAVGTDVRCTVYPHRPDACREVQAGDDKCERARACHGL
                     PPLGPSAPS"
     gene            6440..>6665
                     /locus_tag="PKX01_15430"
     CDS             6440..>6665
                     /locus_tag="PKX01_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77576.1"
                     /translation="MAGKEVSKRQSKPNKNTAITLALLGCVGADALAGTVYAKLSENG
                     RRAREIALLEAARSGRVQTYRDDQAPTLTGT"
BASE COUNT         1039 a         2423 c         2116 g         1087 t
ORIGIN      
        1 accacttacg ggcgttgaag cccgccccca cgccctcgga aaggagaggt gatgatgggc
       61 tacatgatcc acatgaaccg gctgggctcg gcggtcgtgc agaccactta cgggcgttga
      121 agcccgcccc cacgccctcg gaaaggagag gtgatgactt caagccttta cgggcccatg
      181 ctggccctga tcgcgtggac cggtggcgtg gggatgctgg cggcccggcg ccgcgtgcgg
      241 gagatccgcc gccggcgcat tcccctcgcc accctcgccc gcccccgcga cctggcccag
      301 gcgctggacg acacccaggc catggacaac ttcaacaacc tgctgcaggt gccggtactc
      361 ttccacctgt gctgcctggc cttcgcccag caggcgagcg tgccgcaggc ggcggcgatc
      421 ctggcgtggg tctacgtggc cctgcgcatc ggccattcgc tgatccagct cacccacaac
      481 cgggtgctgc cgcgctttgg cgtgtggacc gccggaaacc tggtgctgat gggactgtgg
      541 ggcgtgtttg ccgcggggct tgtgtgacgg cgatggggcg cgcggcgctc ggcgggccgt
      601 cttccgggat atccttgggc cttcccggat tccccaccgc acaccgccgc cgtctgcccc
      661 atgcgcccgc tcttcgccct cctcatcgtc ttcgtcaccg gctgtaccgc cgtcgccgcc
      721 catcgtctgg acgaacgcct cggtccgcgt gatccgggcc gctacgatca ggcgccggcg
      781 cccgtcgccc ccgcaccgga ctactggacc caggtgcgcc cgatcctgga ccggcgctgc
      841 gtgacctgcc acgcctgcta cgacgccccc tgccagctca acctgagccg ctacgacggc
      901 atcacccgcg gcgccagcgc cgagacggtg tattcctcga cgcgcctcac cgcagcgcag
      961 cccacccgcc tgggcttcga cgccctcagc acgcgggcgt ggcgcgagcg gggcttcttc
     1021 ccggtactca acgagcgctc gccaacgccc gaggcgaacc gggagggcag cctgctgctg
     1081 cgcctgctcg ccctcaagca ggcccagccg ggtccgggca gcggcgcgct gccggacaag
     1141 gacttcgatt tctccctgga ccgcagccag gcgtgcgcgc cgctggaggg cgtcggcgcc
     1201 tatgagcgca gccaccccct gtggggcatg cccttcggtc tgccggccct gcgcagcgcc
     1261 gaacacgaca tcctgagccg ctggatcgaa gccggagcgc cgtacacacc gccccccccg
     1321 gcgccgcccg ccgtggcccg ccgcgtggcg gaatgggaag ccctgctcaa cgccgacgcc
     1381 cccggcgccc agctcgccgc ccgctacatc tacgagcact ggtacatcgg ccatctgtat
     1441 ttcgacgagg cgccggacat ccgcttcgag ctggtgcgca gccgcacggc gcccggcgcg
     1501 cccatcgacc ccatcgccac ccgacggccc tacgacgacc ccggcgttgc ccgcgtgtat
     1561 taccgcctgc gccccaacga ggcgacgccg gtggccaaga cctacatgcc cctggtcctc
     1621 gacagcgcac ggctgacgcg gctgcgcaaa tggttcttcg acacgcctgc caccgtggat
     1681 cgcctgcccg gctacgcgcc ggagacggcg gccaatccct tcgccacctt ccgcgccctg
     1741 cccgtggaca gccgttaccg cttcatgctg gacgacgcga agttcacgat gatgggcttc
     1801 atgaagggcc cggtgtgccg cggccaggtg gcgctgaacg tcatcgacga ccacttctgg
     1861 gtgctcttct atgcgccgga aggtgaggtc agccgcaacg tgcagggcct gctggacgcc
     1921 acccagccca acctgcgcat gccggccgag gattccagct ccgtcggcgc cctggcctgg
     1981 ctcgcctacg cgcgggccga acggaactac ctcaacacca agtcggcctt catggccggc
     2041 aagaccgccg tgcgcccgcg tctggaagac ctgtggaccg gcgacgggcg caatcccaac
     2101 gcggcgctga cggtgttccg ccacttcgac agcgcctcgg tggtgcgcgg gctggccggc
     2161 gagcggccgc agaccgtgct gctgctgggc tatcccctgt tcgagcgcat gcactacctg
     2221 ctggtggccg gcttcgacgt gtatggcaac gtcggccacc agctcgccac gcggctctac
     2281 atggacttcc tgcgcatgga aggggaacag aacttcctca ccctgctgcc cctcaaggac
     2341 cgccagaaag tgctcgacca ctggtaccgg ggccgctccg aaccgcgcat ccgccagttt
     2401 gccgacacct cggcctacta tccgggcgag accggcgtgc gcttccacgg accggacagt
     2461 ctcggcgagc tgtaccggct ggcgcagcgc catgtccgcc ccgtgcgcga ggccgggctc
     2521 gattttgccg ggacgggctt cagtccggcg cagggcgcgc tcctcgcccg cctgtcggcg
     2581 gtgaagggcc gcagcgcctc gtggatgccc gaactgagtc tgctggccct gcgcaaggcc
     2641 gacggcagcg tgcagccggt gaccctgatc cgcaacagcg cccacagcaa cgtggcccag
     2701 atgttcaacg aggacgcgcg ccgcctgccg gaggaagaca ccctgcttgc cctgcccggc
     2761 tttgccggcg cctacccgaa cgcgatcttc cagctcgacc cggcggaact gccgcgcttc
     2821 gtcaatgcgg tggaaggcct gacgggcgag gacgatctgc tgcggctggt ccagcgcttc
     2881 ggcatccgcc gcacggacag ccgcttctgg ccggtgagcg acgcgctgca cgacgcctgg
     2941 cagcgcagca gcccccgcga ggcgtcaatc ctggactaca gccggctgga gaatcgctga
     3001 acaccgaccg cgggagacag cggcacgcgc atggccgcgc gccgcgggtc gtcaaaccct
     3061 tagaatgtca gcctgcagga acgaagcttg cttgacgctc cctggaagcc aagcccggcg
     3121 ggcaaattgt caggaaatcg acaggtttgc tccggcaact tccgccccga cccactgccc
     3181 gtctccttcc gtctgcgccc atcatggaat cccactcgat cgaagtcgcc aaacacgtcc
     3241 tcgtcacctt cggcatcatc ctcgccatcg gctccgtctc gggctttgcc agccgcaagc
     3301 tgcgcatgcc cgacgtggcg gtcttcctgc tcgtgggcat ggccattggc ccctcggccc
     3361 tgggctgggt ggacgtcaag gccgattcgg ccctcaacca gatcatcctg atcttcggct
     3421 cgtcctacat cctctttgac ggcggcgcct ccctgcgcct ggccgtgctc aagaacgtgt
     3481 gggtgtccat cgtcctgctg tccaccctgg gcgtgctgat caccgccttc atcaccggct
     3541 acgcggccca gtggatcctc ggcatcccct tcatcgtcgc cctgctgctg gccagcgcca
     3601 tcgcctccac cgacccggcc accctggtgc cggtgttccg tcaggtgcag atccgcgagc
     3661 gggtggccca gacggtcatg tctgaatcgg ccttcaacga cgcggtcggc gccatcctca
     3721 cctttgcggt gctcgggctg gccatgggcc agggcgaatt ctccctgtcc gcgtccctcg
     3781 ccgacctcgc agtacaggcc ggcgtgggcc tggtcgccgg actggtgctg ggctggcttg
     3841 ccgcactctt catcgcccac gagaagtacg cattcctgga ggaatacgcg ccggtggtgt
     3901 ccctgatggc agtgatcggc gcctatctgg gggccgacgg cctgcacgcc agcggcttca
     3961 tggcagtgtt cgtgttcggc atggtgatcg gcaacaagga cgtgctcggc ttcgagatgg
     4021 gtgaggaaga gcagcacaag ctggaggagt tcgtcgccat cacggccctg atcatgcgca
     4081 tgttcatctt catcctgctg ggcagccagg tggatttcgc cctgatgcgg gcccacctgt
     4141 gggaagggct ggcggtggtg gcagtgttca tgttcgtggc gcggcccgcg acggtgttcc
     4201 tgtgcgccct gcccgaccgc aaggcgcgct ggagctggca ggagatgctg ttcatgtgct
     4261 ggacccgcga gaccggcgtg atccccggcg cgctggcggg cctgctgatg ggcatgggcg
     4321 cgccggaagc gccgctgatc gcctcggtga ccttcattgc catcctggtc accatcctgc
     4381 tgcaggccac caccaccaaa tggctggccg gtcgcctcgg cctgcttgaa tgactgcctt
     4441 gcgggcggac gcctggttcg ccacctgaaa ggacaacggc cggaggggtc tccccctccg
     4501 gccgctgccc tgcctccacc cacgcggatt acccaggcat ccgccgccgg tgcatgccca
     4561 gcatcgccag ccccagtgcg cccaggcaca gactgcccgg caccggaacg gcgttcaatg
     4621 tgacggacgc gctgtcctcc acgagaaagt aatacgggat ctggttggca taggccgtcc
     4681 cctgacagcc cagagccgcc agcccggcct gttcgcagac cagttcctcg accgccgtgg
     4741 cgtcaggcgt cgccacgttg tccacgaagc tggcgttgag cgtcacactg gccagctggc
     4801 tcatgtcccg atggtcggcg gtgaacaggt agccgggcaa cagggagccc gccacgagtt
     4861 gcgcggacat gctcacgaga tgggtgccca tgggatcgcc gctcccaccc gtcaccagaa
     4921 agctcgccac atgggtgccg tcgtcggcac cgtagatgcc cgtgctggtg ccgaaatcga
     4981 agctgctgtc cgaatacagg tcgaagccgc cgccgatcag gctgatcagc ccgctcaacg
     5041 gatcggcggt tccccacagg gcataggtca gggtcaggtc gttcgtgccg atcggtgaga
     5101 ccccgtcgga agccacgatg cggaacgcac cgttctcctg gaacatgggc aggccgctgg
     5161 tcggatccat gtactggaac acgaagccgt gtccgccgag gtcgaagctg ctgagcagct
     5221 gggcgcccgc gagaccgctg ccgtccgtgg cgatctggaa gggattggct gcggccggac
     5281 cggcaacgcc caatccggcc agggtgacga cgccaagcgt catcaggcgt gagaaaaacg
     5341 tgttcatcgg aagtgagtcg gaaacaggga tagatggaag acgctgcggc gaccattccg
     5401 ggttgcagga atgggagcaa tgccgcggaa gcattgcacg cacctggcag ggcacgcaaa
     5461 gcaactatgc aaatttgatt gacggcgcca attttacaga tcgccacatt ttattccaga
     5521 tggatttccg ttcagatcgg gcgccggcct gacggcttct ccgccatgcg acggaatggc
     5581 acaatccgcc gcggcccaac tcccccgtca ccatgacccc gccccccgac gacctcagcc
     5641 cgtgccaggc atgcggcgcc tgttgcgccc aattccgggt gtccttctac tggtccgaag
     5701 ccctcgcccg cacgctaccg gacgcctatg tcgagcagat caatccctgg atgagctgca
     5761 tggccggcac gcagggcccc gccccccgct gcgccgcgtt gcacggcgcc gtaggcacgg
     5821 acgtacgctg cacggtctat ccgcatcgcc ccgacgcctg ccgtgaagtg caggccggtg
     5881 acgacaaatg cgaacgggcc cgggcctgtc acggcctgcc gccgctgggg ccctccgccc
     5941 cctcctgatc gccggaccgc agatcgcgcg tgccgccatg gacgggcagt ccggcaacgt
     6001 ctgcgacacg gacacgacgg gcttcattgc atcgacaggc ggtcattctc cacactggaa
     6061 gcttgcgcga agctggcata ctgcttcgac acgcccggtc aggaacccac cacggtcacg
     6121 gatcctcctc gacctgaacc tggcggcgca catgaacgaa cccggtcctg ttcccacctc
     6181 cagcgcagga agattgccca tgtcacttac gctccacgac accccgtccg cccgctgccg
     6241 cagcgtcgca ctgatggtgg ccgccgccct tttcgccggc gcctgtacca ccccgtcttc
     6301 cccttatgcg acgccctcgt cgtcgcccgt gtcgggacct gtcccccggg gcagcgcccc
     6361 ggcccctcag gcatcccagg accagcttgg cgtcacgtgg cggatattga tctgcgggat
     6421 tggcggtggg ctcggttaca tggccggcaa ggaggtttcg aagcggcagt ccaagccgaa
     6481 caagaacacg gccatcaccc tggccttgct cggttgcgta ggcgcggacg cccttgccgg
     6541 caccgtctat gccaagttga gcgaaaacgg ccgacgggcg cgggagatcg cgctactgga
     6601 agccgcccgg tcaggccgag tccagaccta tcgcgacgat caggcgccga cattgaccgg
     6661 cacgg
//
LOCUS       DAOQPA010000154         6553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6727_length_6553_cov_18.196060, whole genome shotgun sequence.
ACCESSION   DAOQPA010000154 DAOQPA010000000
VERSION     DAOQPA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_6553_cov_18.196060"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /locus_tag="PKX01_15435"
     CDS             complement(<1..2482)
                     /locus_tag="PKX01_15435"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMZ77577.1"
                     /translation="MDRRNFLRNSGFLTVSVALGGLAGCGGDSGGLAVPAALGSGWKF
                     PQSVASGDPSPEGAVLWTRVVASSADDVTTASDTGDFSIRVKVTEADNSARLGTTTAL
                     AGALAVDNWIPVYAAYDGTVRNKLTGLKPATTYYYQFEAGMVLSRVGRFRTAPAKGAS
                     VDQLRLAFMTCQDWSVNHWGAFDYIVRNEDVDLIVHLGDYIYETVGASFQSGAVESRH
                     DLLVLPDGAYESGTSGPRYALTLADYRYLYKKYRTDPRLQAVHERFAFVATWDDHEFS
                     DDCWGDAETYDNGSFNSATLSGDNPHQTARRRSANRAWFEFMPADVRLDESTLSFDTI
                     RLYRDLQWGTLAHLVVTDERLYRADHVVPEATPNPATGAALGSIGSRYVVPQSTRDSF
                     EAAKMAAAGSADPLAPVSILGKTQRDWWKSTMTASPATWKLWCNEVSLLRMQLDGTNA
                     IATLFALQAVSTLANSIQGALPAVGGNAAVAGALVAAMTAGASQATAAAAALAIATAA
                     ATAGNPGTAATGAGLTAHQAAIAVAAFTAAAAANGTSAQVAAAARTIAFGYIKPDVQA
                     KGVASSFVQSSGKAAALQGFFTRFLFDCDQWDGYNAERKALMAHLKGNGIQNVVALTG
                     DLHCFDAGVVYDDFDASGGTPVMVDLVTAGISSDSLFTFYADSVGAVNADLATLIYYP
                     LSIPVSGVGTVSLRFNVFDFTMAGHVPTLDELAEQARVPLRGALAAKGVPEASLDATT
                     TAVLAALKADSSFNTDLLGLAQQLAGICANPWIKWAATDAQGYAVVTVTPGRLSCVFK
                     QVNRLVGTAAPSSTVIARTVTATVNAGSA"
     gene            2637..3863
                     /locus_tag="PKX01_15440"
     CDS             2637..3863
                     /locus_tag="PKX01_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HMZ77578.1"
                     /translation="MRAPSPASAALATFRRHPLHFACLATLGLSACGGSDSLTPLPTL
                     DGNAPLVVAHRGASGYLPEETLEAYTKAIDLGADVIEPDLISTKDGVLIARHDPNLAY
                     STDVASHPEFAARKKTTQVDGETQTGWFASDFTLAEIKTLGGISTDAERPQQYNGLYK
                     IVTFQEIIDLAKAKAKETGRSIAVYPETKNPTYHRDLGLPLEDTLIGLINLAGWNSKD
                     APIYVQSFEPSSLKYLRAKGLKTKLIQLIDADDVDFKTGALTYAAPYDKPYDWFKAGD
                     TRLFSAMVTPAGLAEIKTYADGIGPWKRYIVTVKGTLDASGNVKDINGDGKINDADTT
                     SQPATTLIADAHKAGLFVHPYTFRNEQRRLASDYKGDPKNEYLAFYRLGVDGLFSDFT
                     DTAIAARTAYLKEMGR"
     gene            complement(3966..4763)
                     /gene="pstB"
                     /locus_tag="PKX01_15445"
     CDS             complement(3966..4763)
                     /gene="pstB"
                     /locus_tag="PKX01_15445"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMZ77579.1"
                     /translation="MQQATTQSSQGVIKGPAQIAFRNFNFYYGEFLALKNINFEMEKH
                     RVTAFIGPSGCGKSTLLRTINRMYDLYPNQRAEGEILLNGQNLLDPKVDVTTLRARVG
                     MVFQKPTPFPMSIFDNIAFGVSLHEKLTRSEMEERVEWALTKAALWGEVKDKLNQSGM
                     SLSGGQQQRLCIARGIAVKPEVVLLDEPTSALDPISTARIEELVAELKNEFTIVIVTH
                     NMQQAARISDYTAYMYLGELIEYGETDQIFLSPKQKATEDYITGRFG"
     gene            complement(4777..5619)
                     /gene="pstA"
                     /locus_tag="PKX01_15450"
     CDS             complement(4777..5619)
                     /gene="pstA"
                     /locus_tag="PKX01_15450"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMZ77580.1"
                     /translation="MDLLARRKLTNQIALGLSMGAMVFGLFWLAWILVTVLQLGLGGL
                     SLSLFTEMTPPPGAETGGLLNAIVGSLILVALGTAVGAPIGIMAGIYLAEYGRYTWLG
                     RVTRFVNDLLLSAPSIVIGLFVYAVIVATTGKFSAWSGAVALALLVLPIVIRTTENML
                     MLVPGSLREAAFALGAPKHIVISRVTLRAARSGVVTGVLLAIARIAGETAPLLFTALS
                     NQFWSVDLNEPIANLPVTIFKFAMSPFEDWQRLAWAGVLLITLGVLGLNIIARVMFRA
                     EKLH"
     gene            complement(5623..6540)
                     /gene="pstC"
                     /locus_tag="PKX01_15455"
     CDS             complement(5623..6540)
                     /gene="pstC"
                     /locus_tag="PKX01_15455"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HMZ77581.1"
                     /translation="MGAKVFGGISTTFAVLALALLAGIIVALTVASWPSIVAFGPAFL
                     VTAEWNPPAEQFGALIPIYGTLVSSIIALVVAVPVSFGIALFLTELSPLWLRRPLGTA
                     IELLAAVPSIVFGMWGLLVFAPIFGEYIEPALGATLGKIPLLGALFSGPSLGIGLLCA
                     GIILAIMIIPYIASVMRDVFEVTPAMLKESAYGLGCTTWEVMFRVVLPYTKNGVVGGV
                     MLGLGRALGETMAVTFVIGNTNFLSTASLFAPGNSITSALANEFAEAERGLHTSALME
                     LGLILFVITLIVLVLSKLLLLRLSANEGR"
BASE COUNT         1165 a         2189 c         2205 g          994 t
ORIGIN      
        1 ccgcgctgcc tgcattcacc gtcgcggtca ccgtccgggc aatcaccgtg ctcgacggcg
       61 cggccgtgcc caccagccgg ttcacctgct tgaagacaca gctcaggcga cccggcgtca
      121 ccgtgaccac ggcatagccc tgggcgtcgg tcgccgccca ctttatccac gggttggcgc
      181 agatgcccgc cagttgctgg gccagcccca gcaggtcggt gttgaagctg ctgtccgcct
      241 tgagcgcagc gaggaccgcg gtcgtggtgg cgtccaggga cgcttccggc acacccttgg
      301 cggccagggc gccgcgcagg ggcacacggg cctgttcggc cagttcgtcc agcgtcggca
      361 cgtgaccggc catggtgaag tcaaatacgt tgaagcgcag gctcacggtg ccgacgccgc
      421 tcaccggaat gctcagggga tagtagatca gggtggccag atccgcattc acggcgccga
      481 cggaatcggc gtagaaggtg aacagggagt cggagctgat cccggcggtc accagatcga
      541 ccatcactgg cgtgccaccg ctggcgtcga agtcgtcgta caccacgccg gcgtcgaaac
      601 aatgcaggtc gccggtgagc gccaccacat tctggattcc gttgcccttg agatgggcca
      661 tcagcgcctt gcgctccgcg ttgtagccgt cccactgatc gcagtcgaac aggaagcggg
      721 tgaagaagcc ctgcagtgcg gcagccttgc cggaactctg cacgaagctc gatgcgacgc
      781 ccttggcctg gacgtccggc ttgatgtagc cgaaggcgat ggtgcgggcg gcggcggcga
      841 cttgcgcgct ggtgccgttg gccgcagccg cggcagtgaa ggcggccacc gcgatggccg
      901 cctggtgagc ggtgaggccg gcccccgtcg ctgcggtgcc aggattgccc gccgtcgccg
      961 ctgcggtggc gatggccagc gctgcggcgg ccgccgtcgc ctggcttgcc cctgcagtca
     1021 tcgccgccac cagcgcgccg gccacggccg cgttgccgcc caccgccggc agcgcaccct
     1081 ggatggaatt cgccagcgta ctcaccgcct gcagggcaaa cagggtggcg atggcattgg
     1141 tgccgtccag ttgcatgcgc agcagggaca cctcattgca ccacagcttc caggtggcgg
     1201 gcgaggcggt catggtggac ttccaccagt cccgctgggt cttgcccagg atggaaaccg
     1261 gcgccagcgg gtcggccgaa ccggcggccg ccatcttggc tgcctcgaag ctgtcgcggg
     1321 tgctctgggg cactacgtag cgggagccga tggagcccag cgcggcgccg gtggcggggt
     1381 tgggcgtggc ctccggcacc acgtggtcgg cacggtacag gcgctcgtcg gtcaccacca
     1441 gatgggccag cgtaccccac tggaggtcgc gatacaggcg gatcgtatcg aagctgagcg
     1501 tgctctcgtc caagcgcacg tcagccggca tgaactcgaa ccaggcccgg ttggcgctgc
     1561 gccggcgggc ggtctgatgg gggttgtcgc cgctcagggt ggcgctgttg aagctgccgt
     1621 tgtcataggt ctcggcgtcg ccccagcaat catcggagaa ctcgtggtcg tcccaggtgg
     1681 cgacgaaggc aaagcgctca tgcacggcct gcaggcgcgg atcggtgcgg tacttcttgt
     1741 ataggtagcg gtaatcggcc agggtcaggg cgtagcgcgg gccgctggtg cccgattcgt
     1801 aagcgccgtc gggcagcacc aggaggtcgt ggcgggattc caccgcgccg ctctggaagc
     1861 tcgcacccac cgtctcgtag atgtagtcgc ccagatggac gatcaggtcc acgtcctcat
     1921 tcctgacgat atagtcgaac gccccccagt gattgacgct ccagtcctgg caggtcatga
     1981 aggccaggcg cagctgatcg acgctggcgc ccttggccgg cgcggtgcgg aagcggccga
     2041 cccgggacag caccatgccg gcctcgaact ggtagtagta ggtggtggcg ggcttgaggc
     2101 cggtgagctt gttgcgcacg gtaccgtcgt aggccgcata gaccgggatc cagttgtcca
     2161 ccgcgagggc gccggccagc gccgtggtgg tgccgaggcg tgccgaattg tcggcttcgg
     2221 tcaccttcac gcggatcgaa aaatcgcccg tatcggacgc cgtggtcacg tcatcggccg
     2281 aggacgccac cacgcgggtc cacagcaccg cgccctcggg cgacggatcg ccggaggcca
     2341 cgctctgggg gaatttccag ccgctgccca gggctgccgg cacggccagg ccgccggagt
     2401 cgccaccgca gcctgccagg ccgcccaggg cgacggagac cgtcagaaat ccgctgttgc
     2461 gaaggaagtt gcgtcgatcc atggcctggg gctcctgtca gaaagttcag cccgacagcc
     2521 taagccccga gaatttcagg cgtgttgccc cctccatgaa gggaaattca tgcgcccatc
     2581 acgcgctgtt cacgaagagt cgccagcctc ggagcttttc ctgaccggag actttcatgc
     2641 gcgccccctc ccctgcatcc gctgccctcg cgaccttccg ccgccacccc ctgcacttcg
     2701 cctgcctcgc caccctgggc ctgtctgcct gtggcggcag cgattccctc acgcccctgc
     2761 ccaccctgga cggcaacgcg ccgctggtgg tcgcccaccg gggcgcctcc ggctacctgc
     2821 ccgaggagac cctggaggcc tacaccaagg ccatcgacct gggcgccgac gtgatcgagc
     2881 cggacctgat ttccaccaag gacggcgtcc tcatcgcccg ccacgatccc aacctggcct
     2941 acagcaccga cgtggccagc cacccggaat tcgccgcccg caagaagacc acccaggtgg
     3001 acggcgagac ccagaccggc tggttcgcca gtgacttcac cctggccgag atcaagaccc
     3061 tgggcggcat ctccaccgat gccgaacgcc cgcagcagta caacggcctg tacaagatcg
     3121 tcaccttcca ggagatcatt gatctggcca aggccaaggc caaggaaacc ggccgcagca
     3181 tcgccgtcta ccccgagacc aagaacccca cctaccaccg cgacctgggc ctgcccctcg
     3241 aagacaccct catcggcctg atcaacctgg ccggctggaa cagcaaggac gcgccgatct
     3301 acgtgcagtc cttcgagccc tccagcctca agtacctgcg cgccaagggc ctgaagacca
     3361 aactgatcca gctcatcgac gccgacgacg tggacttcaa gaccggcgcc ctcacctacg
     3421 ccgcccccta cgacaagccc tacgactggt tcaaggccgg cgacacccgc ctgttcagcg
     3481 ccatggtcac gccggcgggc ctcgccgaga tcaagaccta cgccgacggg attggcccgt
     3541 ggaagcgtta catcgtcacc gtcaagggca cgctcgacgc cagcggcaac gtgaaggaca
     3601 tcaacggcga cggcaagatc aacgacgccg acaccaccag ccagcccgcc accacgctga
     3661 tcgccgacgc ccacaaggcc ggcctgttcg tgcaccccta caccttccgc aacgagcagc
     3721 gccgcctcgc ctccgactac aagggcgacc cgaagaacga gtacctggcc ttctaccgcc
     3781 tcggcgtgga tggcctgttc tcggatttca ccgacacagc catcgccgcc cgcacggcct
     3841 acctgaagga gatgggccgc tgaggcagcg cgaccgagcc gccgcggccc cgcggggcgg
     3901 cggcggtgaa cgcacaaacg cccggacacg tccgggcgtt tgtgttcgac ggagggcgcc
     3961 gacgatcagc cgaagcggcc ggtgatgtag tcttcggtgg ccttctgctt gggcgacagg
     4021 aagatctggt cggtctcgcc gtactcgatc agctcgccca ggtacatgta ggcggtgtag
     4081 tcggagatcc gcgccgcctg ctgcatgttg tgggtcacga tgacgatggt gaactcgttc
     4141 ttcagctccg cgaccagctc ctcgatgcgg gccgtggaga tcgggtccag cgccgaggtg
     4201 ggctcgtcga gcagcaccac ttccggcttc accgcgatgc cgcgggcgat gcacagacgc
     4261 tgctgctgac cgccggacag gctcatgccg ctctggttga gcttgtcctt cacctcgccc
     4321 cacagggccg ccttggtgag ggcccactcc acccgttcct ccatctcgga gcgcgtcagc
     4381 ttttcgtgca gggacacgcc gaaggcgatg ttgtcgaaga tggacatggg gaacggggtc
     4441 ggcttctgga acaccatgcc gacgcgcgcc cgcagggtgg tgacgtccac cttcgggtcg
     4501 agcaggttct gcccgttgag caggatctcg ccctcggccc gctggttggg gtagaggtcg
     4561 tacatccggt tgatggtgcg cagcagcgtg gacttgccac agccggaggg accgatgaag
     4621 gccgtgaccc ggtgcttctc catctcgaag ttgatgttct tcagcgccag gaattcgccg
     4681 tagtagaagt tgaagttccg gaaggcgatc tgcgccgggc ccttgatgac accctggctg
     4741 gattgagtgg tggcttgttg catggcagag gctcgcttag tggagttttt cggcgcggaa
     4801 catcacgcgg gcaataatgt tgaggcccag gaccccgagg gtgatcagca gcacgccggc
     4861 ccaggccaga cgttgccagt cctcgaaggg gctcatggcg aacttgaaga tcgtcaccgg
     4921 caggttggcg atcggctcat tcagatccac gctccagaac tggttggaca gggcggtgaa
     4981 gagcagcggc gcggtctcgc cggcgatgcg cgcaatggcc agcagcacgc cggttaccac
     5041 gccgctgcgg gcggcgcgca gggtcacgcg ggagatcacg atgtgcttgg gcgcgcccag
     5101 ggcgaaggcc gcttcccgca gggaaccggg caccagcatc agcatgttct cggtggtgcg
     5161 gatcacgatg ggcagcacca gcagggccag ggcgacggcg ccggaccagg ccgagaactt
     5221 gccggtggtg gcgacgatca ccgcatacac gaagagaccg atgacgatgg acggcgccga
     5281 caggagcagg tcgttcacga agcgggtgac gcggcccagc caggtgtagc ggccgtattc
     5341 cgccaggtag atgccggcca tgatgccgat cggggcgccg acggcggtcc ccagggccac
     5401 caggatcagg ctgccgacaa tggcattgag cagaccgccg gtctcggcgc cgggcggcgg
     5461 cgtcatctcg gtgaacaggg acagggacag gccaccgagg cccagctgga gcacggtgac
     5521 gaggatccag gcgagccaga acaggccgaa gaccatggcc cccatggaga ggccgagggc
     5581 gatctggttg gtgagtttgc gacgggcgag caggtccatg gatcagcgtc cttcgttggc
     5641 ggacaggcgg agcagcagga gcttggacag caccagcacg atcagggtga tgacgaacag
     5701 gatcaggccc agctccatca gcgccgaggt atgcaggccc cgctccgctt ccgcgaattc
     5761 attggccaga gccgaggtga tgctgttgcc cggcgcgaac agcgaggcgg tcgacaggaa
     5821 gttggtgttg ccgatcacga aggtcaccgc catggtttcc ccgagggcac ggccgaggcc
     5881 cagcatcacg ccgcccacca caccgttctt ggtgtagggc aggaccacgc ggaacatcac
     5941 ctcccaggtg gtacagccga gaccgtaggc ggactccttg agcatcgccg gcgtcacctc
     6001 gaacacgtcg cgcatcaccg atgcgatgta gggaatgatc atgatcgcca ggatgatgcc
     6061 cgcacacagc aggccgatgc ccagcgacgg accggagaac agcgcgccga gcagcggaat
     6121 cttgcccagg gtggcgccca gggcgggctc gatgtattcg ccgaagatgg gcgcgaagac
     6181 cagcaggccc cacatgccga agacgatgga cggcacggcg gcgagcagct cgatggcggt
     6241 gcccagcggg cggcgcagcc acaggggcga cagttcggtg aggaacaggg cgatgccgaa
     6301 gctcaccggc acggccacca ccagggcgat gatcgacgat acaagggtgc cgtagatggg
     6361 aatcagggcg ccgaactgct cggcgggcgg gttccattcc gcggtcacca ggaaggccgg
     6421 gccgaaagcg acgatgctcg gccacgacgc aacggtcagg gccacaatga tgccggccag
     6481 cagggccagg gccagcaccg cgaaggtggt cgagatgccg ccgaagacct tcgcgcccac
     6541 ctggctgacc ggc
//
LOCUS       DAOQPA010000155         6415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_6955_length_6415_cov_18.897327, whole genome shotgun sequence.
ACCESSION   DAOQPA010000155 DAOQPA010000000
VERSION     DAOQPA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_6415_cov_18.897327"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PKX01_15460"
     CDS             <1..908
                     /locus_tag="PKX01_15460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ77582.1"
                     /translation="PHRGRRDEARPFSGGNPDAPAPAGRPRPVARPDAPPRHERKPWN
                     GEVRPAPAPSPLRIDGSIRLSKRMTELGLCSRREADEYIEKGWVRVDGRVVSELGARV
                     KPGQTIELDRRGQTAQERRVTILLNKPVGYVSGQAEDGYEPASVLLTPENRWAEDPNE
                     TRWLRTHLKGLAPAGRLDIDSVGLLVFTQDGRIARALIGDDSDVEKEYHVRVEGRLPP
                     EGLALLNHGLSLDGQALRPAKVSWLNEDQLRFVLREGKKRQIRRMCELVGLKVVGLKR
                     VRIGRVLLGPLPPGQWRYLAAGEKF"
     gene            complement(928..1836)
                     /locus_tag="PKX01_15465"
     CDS             complement(928..1836)
                     /locus_tag="PKX01_15465"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ77583.1"
                     /translation="MKAWNGWCSAARWAVVALAMVWSGLAAARGPLFLWEVLDAAGTV
                     RAYLYGTIHVCDAACFPLPARVTEAFARSDSLALELDPVDPALAGVLARAGVLPEDQR
                     LDDRLPAALRPRLTAAARLLGIPPAVLQRLQPWMASTVLTLRAAAQAGFVTEQGIDLW
                     LARAAQARGLPLRPLETVERQLAALSAGGDAAQAAGVEEVVDLVENEAGREYFGALVQ
                     AWRAGDVGELDHLMREVTSSPAMAPLLEDMLDARNREMAETLVGRLTPGARPFVAVGA
                     GHFGQDSGLLAQLARRGFRLRQVEAD"
     gene            1876..2841
                     /gene="miaA"
                     /locus_tag="PKX01_15470"
     CDS             1876..2841
                     /gene="miaA"
                     /locus_tag="PKX01_15470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMZ77584.1"
                     /translation="MMPDSASLPPAVLLMGPTASGKTACALALARHLPVEIVSVDSAL
                     VYRDMDIGTAKPDAAERAACPHHLIDLISPEEAYSAARFCEDARRLMAEISARGRLPL
                     LVGGTMLYYKALRDGLSELPEADPELRRAIEDTAARDGWPVLHAELARLDPDAAARLA
                     PHDAQRIQRALEIVRLTGAPLAEAYARRAAAVATHRFIPVALTPSDRSVLHARIAQRF
                     DAMLAAGFVEEVETLRRRYVLTPEMPSMRCVGYRQALEYLEGRVDHATFRDKGIFATR
                     QLAKRQITWQRNFRDTWGNLAELDSLAADLPERLHRHVARSLDAR"
     gene            2866..3012
                     /locus_tag="PKX01_15475"
     CDS             2866..3012
                     /locus_tag="PKX01_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77585.1"
                     /translation="MADYSADILLERGGQDFLDRRYSRTHRLRFDGSLDIAGSASPHV
                     VRPP"
     gene            3375..4463
                     /locus_tag="PKX01_15480"
     CDS             3375..4463
                     /locus_tag="PKX01_15480"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HMZ77586.1"
                     /translation="MQRIDTLIPPGRPAARVELLLGDLTALDAEYALDVLVVSAFPGD
                     YLPTPGSLIGALHRKGLSVEALAAEKDVDLRSAYACWLSRPLAPTDPGLRFSRILCFE
                     PATRGQPPEVVGDIFRALTPILAERPEIRRIGLPVVAAGDQGYAIPTMLAPLLEAAVH
                     WITRGLPIERLSIVLYDAADVAAARETFERFKAQLTTPVPQDLSRRLSPEADAATPEP
                     ATVAPDYDVFISYSHKNTAASEALEAALRRLQPDLRIFVDRKDLDIGCAWQPEIFETL
                     DRCRKVVALLSPDYLASKVCKEEFNIAWIRAREADQDIIFPLYLFSAALPTYMKYRNF
                     FDCREGDADKIEAASKVLLKALASGPTA"
     gene            4521..5045
                     /locus_tag="PKX01_15485"
     CDS             4521..5045
                     /locus_tag="PKX01_15485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ77587.1"
                     /translation="MRPPLPLLLVALFVTIGFGRPVQAGDPLPEAAPSGPPESPAASA
                     SEPPPNTTPVPLPLRPADMNWASPSGQDAIRESWMMGGEALAGPYALRVRLTAGARVP
                     VHRHPDDRVVTVLAGTVHAGFGGIFDETRTVAMQTGTVFLVPADESHYLWARDGDAEY
                     QENGVGPTGQSRGW"
     gene            complement(5052..5822)
                     /locus_tag="PKX01_15490"
     CDS             complement(5052..5822)
                     /locus_tag="PKX01_15490"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HMZ77588.1"
                     /translation="MVSSIGSSSYSSYAVSRPDPAQMASKLFSKLDTKGQGYLSETDL
                     QSAFNQMFTSATGGSSSASSTSGTDSEASQLFKALDSDGNGQVTKSEFTSGLQKLAEA
                     LDNQASSSRMGQAHGAGGMAPPPPPQGDGDGDGDDGVSGAGLTKDQLTSQLQQIGDTD
                     SQRSSLISKIVANFDKADTNGDGKVSAQEAMAYDRSSRSSGTAATASAEGTSSSSNQL
                     SDARLLHRVMELMRAYDVNGDAAGNAATGSSGLTAVSA"
     gene            6335..6409
                     /locus_tag="PKX01_15495"
     tRNA            6335..6409
                     /locus_tag="PKX01_15495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6367..6369,aa:Val,seq:cac)
BASE COUNT          992 a         2487 c         1991 g          945 t
ORIGIN      
        1 ggcctcatcg cggccgccgc gatgaggccc gccccttctc tggcggcaat ccggacgccc
       61 ccgcgcccgc cggccggccg cgtcctgtcg cccgtcccga cgcaccgccc cgccacgaac
      121 gcaaaccctg gaacggcgaa gtccgcccgg cgccggcccc gtcgcccctt cgcatcgacg
      181 gctcaatccg cctctccaaa cgcatgaccg aactgggtct gtgctcccgc cgtgaagcgg
      241 acgaatacat cgagaagggc tgggtgagag tggatggccg cgtggtcagc gagctgggcg
      301 cccgcgtcaa gcccggccag acgatcgaac tggatcgccg cggccagacc gcccaggagc
      361 gccgcgtcac gattctcctc aacaaacccg tcggctacgt gtccgggcag gccgaggacg
      421 gctacgaacc cgccagcgtg ctcctcaccc cggagaaccg ctgggccgaa gatcccaacg
      481 agacccgctg gctgcgcacc cacctgaagg gcctcgcccc cgccggccgg ctggacatcg
      541 actcggtggg cctgctggtc ttcacccagg acggccgcat cgcccgcgcc ctgatcggcg
      601 acgattcgga tgtggaaaag gagtaccacg tgcgcgtcga aggtcgcctc ccgccggagg
      661 gcctcgccct gctcaaccat ggcctgtccc tggacggcca ggccctgcgt ccggccaagg
      721 tgagctggct caacgaggac cagctgcgct tcgtgctgcg ggaaggcaag aaacgccaga
      781 tccgccgcat gtgcgaactg gtgggcctga aggtggtcgg tctcaagcgc gtgcgcatcg
      841 gccgcgtact cctcggtccg ctgccgccgg ggcaatggcg ctacctggca gcgggcgaga
      901 agttctgagg ccccgcctcg tcgcgcctca gtccgcctcc acctgacgca gacggaatcc
      961 ccgccgcgca agttgtgcca acaggccgga atcctggccg aaatgtcccg cgcccacggc
     1021 cacgaagggc cgcgcaccgg gcgtgaggcg cccgacgagc gtctcggcca tctcccggtt
     1081 gcgggcgtcc agcatgtcct ccagcagtgg cgccatcgcg gggctggacg tcacctcccg
     1141 catcaagtgg tcgagttcgc ccacgtcgcc ggcccgccag gcctgcacga gggcaccaaa
     1201 atattcccgc ccggcctcgt tctccaccag atcgaccacc tcctccacgc ccgccgcctg
     1261 ggcagcgtcg ccgccggccg acagggccgc cagctggcgc tccaccgttt ccagcggacg
     1321 caggggcagg ccgcgggcct gggcggcgcg ggccagccac aagtcgatcc cctgctccgt
     1381 gacgaaacca gcctgggctg ccgcccgcag ggtgagcacc gtggacgcca tccacggctg
     1441 caggcgttgc aggacggccg gcggaatccc cagcaggcgg gccgccgcag taaggcgcgg
     1501 gcgcagggcc gccggcagcc ggtcatccag gcgctggtcc tccggcagca cgccggcacg
     1561 ggccagcacg cccgccaggg ccggatccac aggatccagt tccagcgcca ggctgtcgga
     1621 ccgggcgaag gcttccgtca ctcgcgccgg cagcggaaag caggccgcgt cgcagacgtg
     1681 gatcgtgcca tacaggtagg cgcgcaccgt accggcggcg tccagcacct cccacaggaa
     1741 gagcggcccc ctcgccgcag ccagcccact ccacaccatc gccagcgcca cgaccgccca
     1801 ccgcgccgca ctgcaccaac cgttccacgc cttcatgcca ctcccgctac aatgcgccac
     1861 cctgatccag tcgccatgat gcccgattcc gcctccctgc cgccggccgt cctgctgatg
     1921 ggccccaccg ccagcggcaa gaccgcctgc gccctggccc tcgcccgcca tctgccggtg
     1981 gagatcgtca gcgtggattc ggccctggtg tatcgcgaca tggacatcgg caccgccaag
     2041 ccggacgccg ccgagcgggc cgcctgcccc catcacctga tcgacctgat cagcccggaa
     2101 gaggcctatt ccgctgcccg cttctgcgag gatgcacgcc gcctgatggc cgagatcagc
     2161 gcccgcggtc gcctgcccct actggtgggc ggcaccatgc tctactacaa agccctgcgg
     2221 gacggcctgt cggaattgcc ggaggcggac cccgaactgc gccgcgccat cgaagacact
     2281 gccgcgcggg acggctggcc ggtcctgcat gccgaactcg cccgcctcga cccggacgcc
     2341 gcggcgcgcc tcgcgcccca tgacgcccag cgcatccagc gtgccctgga gatcgtgcgg
     2401 cttaccggcg cgcccctcgc cgaggcctac gcccgccgcg cggccgcagt tgctacccac
     2461 cgcttcatcc cggtcgccct caccccctcg gaccgcagcg tgctccacgc acgcatcgcc
     2521 caacgcttcg acgccatgct ggcggccggc ttcgtggaag aagtcgagac gctgcgccgc
     2581 cgctacgtcc tcacgcccga gatgccctcc atgcgctgcg tgggttaccg ccaggcgctg
     2641 gaatacctgg aaggtcgcgt ggatcacgcc actttccgcg acaagggcat cttcgccacc
     2701 cgccagctcg ccaagcgcca gatcacctgg cagcgcaact tccgcgacac ctggggcaat
     2761 ctggcggaac tggacagcct cgccgccgac ctgcccgagc ggctccaccg ccacgtggcg
     2821 cggtccctcg acgcccgctg aatcccgacc gaaaggcagc gaccgatggc cgactactcc
     2881 gccgacatcc tgttggaacg gggcgggcag gactttcttg accgccgcta cagccgcacc
     2941 caccgtctgc gtttcgacgg cagcctggac atcgccggct ccgcctcgcc ccacgtggtg
     3001 cggccaccct aggccgaccc gtccgccgtc gaccccgaag aagccttcgt cgccgcgctg
     3061 gcgagctgtc acaggctgtg gttcctgcac ctggcctgcg aaaagggcta ccgggtggac
     3121 cggtacaccg acggcaagct gtggatgagc cacgtcaccc tgcgtccggc cgtgcgcttt
     3181 gccggcacgc cgtgtcccga cgcagcagcc ctcgccaccc tccaccacac ggcccacacc
     3241 gaatgcttca tcgcccgttc ggtcaccggg ttgagccggt gctcgatccc gcctgagccc
     3301 cgccgccacg acaggccgcc gctgccgggc tatgctgaaa tgatgacatt gccccgcaca
     3361 acggagatcc caccatgcag cgcatcgaca ccctcatccc gccgggcagg cccgctgcgc
     3421 gggtcgaact cctgctcggc gacctgaccg ccctcgacgc cgaatacgcc ctggacgtcc
     3481 tcgtcgtctc cgcctttccc ggcgactacc tgcccacgcc cggctccctg atcggcgccc
     3541 tgcaccgcaa gggcctgtcg gtggaagccc tggccgccga aaaggacgtg gacctgcgca
     3601 gcgcctacgc ctgctggctg tcccggcccc tggcgccgac cgatccgggc ctgcgctttt
     3661 cacgcatcct gtgcttcgag ccggcgaccc gcggccagcc gcccgaggtg gtgggcgaca
     3721 tcttccgtgc cctgacgccg atcctcgccg agcggccgga aatccgccgc atcggcctgc
     3781 ccgtcgtggc ggcgggcgat cagggctacg ccattcccac catgctcgcc ccgctccttg
     3841 aggccgccgt gcactggatc acccgcggcc tgcccatcga gcggctgtcc atcgtcctct
     3901 acgacgccgc cgacgtggct gccgcacgcg agacctttga gcgattcaaa gcgcaactga
     3961 caacccctgt cccccaggac ctcagccgac gcctctcgcc ggaagccgac gccgccacgc
     4021 cggaacccgc cacggtggcg cccgattacg acgtcttcat cagctattcc cacaagaaca
     4081 ccgcggccag cgaagccctc gaagcggccc tgcgccgcct gcagcccgat ctgcgcatct
     4141 tcgtggatcg caaggacctg gacatcggct gcgcctggca gccggagatc ttcgaaaccc
     4201 tcgaccgctg ccgcaaggtc gtcgccctgc tgtcgcccga ctacctggcc tccaaggtct
     4261 gcaaggagga gttcaacatc gcctggatcc gcgcccgcga ggccgaccag gacatcatct
     4321 ttcccctcta cctgttctcg gcggcgctcc ccacctacat gaagtaccgc aacttcttcg
     4381 actgccgcga aggcgacgcg gacaagatcg aagccgccag caaggtcctg ctgaaggccc
     4441 tcgccagcgg tccgacggcg tgagcggcgc ggtatcatcg ccgtcacccg cccgtcgccc
     4501 acaattcccg gaggcccgtc atgcgcccac cgttgccgct gctgctcgtc gccctcttcg
     4561 tcacaatcgg gtttgggcgg cctgttcagg caggcgatcc cctgcccgag gcggccccgt
     4621 caggcccccc ggagtccccc gcagccagcg ccagcgaacc gccgcccaac accacgccgg
     4681 tgccactacc cctgcgcccg gccgacatga attgggcgtc cccgtcggga caggacgcga
     4741 tccgcgaaag ctggatgatg ggcggcgaag cgctcgccgg cccctacgcg ctgcgggtcc
     4801 ggctgacggc cggcgcgcgg gtgcccgtgc atcgccaccc ggacgaccgg gtggtcaccg
     4861 tgctcgccgg caccgtgcac gccggcttcg gcggcatctt cgacgaaacc cgtaccgtgg
     4921 ccatgcagac gggcacggtc ttcctcgtcc ccgccgacga atcccactat ctgtgggccc
     4981 gggacgggga cgcggaatat caggagaacg gcgtcggccc gacaggccag agccggggct
     5041 ggtgagatcc ctcaggccga cacggccgtg agccctgacg acccggtggc ggcgttgccg
     5101 gccgcatccc cattcacgtc ataggcacgc atcagctcca tcacccgatg cagcaggcgc
     5161 gcgtcgctga gctgattgga ggacgagctc gtcccctcgg cgctggccgt ggccgcggtc
     5221 ccggaactgc ggctgctgcg gtcatacgcc atggcctcct gggcgctcac cttgccgtcg
     5281 ccattggtat ccgccttgtc aaaattggcg acgatcttcg aaatcaggct ggagcgctga
     5341 ctgtccgtat cgccaatctg ctggagctgg ctggtcagtt gatccttggt caggccggcg
     5401 ccggacaccc catcgtcccc atcgccgtcg ccatccccct ggggcggcgg tggcggcgcc
     5461 atgccgcccg ctccgtgggc ctgccccatg cggctgctcg aggcctgatt gtccagggcc
     5521 tccgccagct tctggaggcc ggacgtgaac tcgctcttcg tgacctggcc gttgccgtcc
     5581 gaatccagcg ccttgaagag ctgactcgcc tcgctgtcgg tcccgctggt ggacgacgcg
     5641 gaggatgaac cacccgtcgc cgacgtgaac atctggttga aggccgactg gagatccgtc
     5701 tcgctgagat agccctggcc cttcgtgtcg agcttcgaga agagtttgct cgccatctgt
     5761 gccgggtcgg gacgactcac ggcgtaactg gaatagctgg aactgccgat gctgctgacc
     5821 atgatggaat cctttcctgt ctgagcgggc accgcccgcg ggacaggccg ccgcgtcctg
     5881 aacggacgcc gaggccctgc ccggtcctga gcaacttatc ggccggaaac ccaccggatt
     5941 cgcggcgatt caggttaagc cgggtaaagt tcggttaaga ctccatggcg ccgggacggt
     6001 cggtggcggc ggtgaatcac cctctgaccg gcgcgggatc gtcccggccc tccgacctgg
     6061 cacatcctgc cggtcgttct ccctgcaggt cgccggaacg gcaaggaaac tccatgagaa
     6121 gccctggctc gccaggggaa gctccggtac gcggcgcgcg cgcaggcgcc acccggcaaa
     6181 tcctgccgcc ctgatccacg ctgcgccgcc ctggcgcgga accaccggat tgccgtccgc
     6241 gcccgaatgg cgggagatgc cgtctattgg cctgttttcg cagaaaaggc ttgacggaca
     6301 gcaggtccac gtctaatatt cgcgcctctc ttccgggcgg ttaactcagc ggtagagtgc
     6361 caccttcaca cggtggaagc cactggttcg atcccagtac cgcccaccag attcc
//
LOCUS       DAOQPA010000156         6366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7065_length_6366_cov_16.754239, whole genome shotgun sequence.
ACCESSION   DAOQPA010000156 DAOQPA010000000
VERSION     DAOQPA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7065_length_6366_cov_16.754239"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKX01_15500"
     CDS             <1..939
                     /locus_tag="PKX01_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ77589.1"
                     /translation="RFYTRLDSMSRIVSVTQKAAAGAKRIFDILDHVSSVPEPVNPQH
                     LPKVTGAIEVRNVGFRYGNRTILRNVDLSIQPGEMIGLVGHSGSGKSTLVNLICRFYD
                     VTEGSIRIEGVDLRAVPVAEYRQNIGLVLQEPFLFFGTIADNIAYGKPDATREEIIAA
                     ARAAHAHDFIMRLPFGYDSLVGERGQALSGGERQRISIARALLIDPRILILDEATSAV
                     DTETEMEIQAALENLIRGRTTLAIAHRLSTLRKADRLVVLDRGQIVEIGNHDTLMDKQ
                     GAYFRLYEAQTRKLDDDALGGGTPLPAAAHRQHAQA"
     gene            961..1428
                     /locus_tag="PKX01_15505"
     CDS             961..1428
                     /locus_tag="PKX01_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HMZ77590.1"
                     /translation="MSQPPLNLARNPFGQLVFTAADGTPHTVVPVRAFPIAAPAENIG
                     LINPEGEEVAWVDRLADLPDAPRRLIEDELASREFMPVIQRIRHCSSFATPSTWELDT
                     DRGPATLVLKGEEDIRRLKSPSLLIADSRGIHFLVRDRYALDAHSKRILDRFL"
     assembly_gap    1438..1537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1641..2237
                     /locus_tag="PKX01_15510"
     CDS             1641..2237
                     /locus_tag="PKX01_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77591.1"
                     /translation="MKNFLRALEAQRWDDHRFYHHSRINQTLHFISAISFVVAYCLIF
                     TDPVKAALIGWLVSMVTRQSGHFFFEPLGYDEENQCTNEYKEAVKVGYNLQRKVVLLS
                     LWAATPVVLYMDPTCFGLFDAHVDMDGYIDHLGKLWLSLGVAGLLFRTVHLFFLKDVQ
                     TGLVWLTKILTDPYHDIKLYHRAPLELLRELRARRQTA"
     gene            complement(2382..4037)
                     /locus_tag="PKX01_15515"
     CDS             complement(2382..4037)
                     /locus_tag="PKX01_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ77592.1"
                     /translation="MEDALVWAGLGALALPGLALKIKRRLELSKGKHPSLAGHAKMSR
                     RVARLLPFYEYDEAGFFNSDDAPAEVVARRREGFFRLAELYRARFARSAALTAEAAEG
                     VSDLQFTGAYRVPFQYSRLVREHLKGGSFYTASAGVTVTDADGNVFYDLAGSYGVNVF
                     GYDFYKTCMARGAERVAALGPVLGSYHPVLLENVRRLKAVSGLDEVSFHMSGTEAVMQ
                     AVRLARYHTRRNHLVRFCGSYHGWWGDVQPGVGNPVAAEHTYTLRDMHPDALRVLRRR
                     KDIACVLINPLQGLHPNAAAPGDSSLVDSSRRAGFDRAAYTAWLKELRQVCTERGIVL
                     IFDEVFLGFRLAAGGAQEYFGVQADMVTYGKTLGGGLPVGVVCGRRDLMKRFRDDAPA
                     DICFARGTFNSHPHVMGAMAEFLARLETPEVKAVYAGLDDTWNGRAARLNARLLEAGL
                     PVQVANMQSVWTVFYTRPSRYNWMLQYYLRAEGLALSWVGSGRFIFSLNYSDADFEAV
                     AERFVAAAKQMEADGWWWHDGRTTNKDIRRRILKEMLRKRFLG"
     gene            4062..4967
                     /gene="asd"
                     /locus_tag="PKX01_15520"
     CDS             4062..4967
                     /gene="asd"
                     /locus_tag="PKX01_15520"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HMZ77593.1"
                     /translation="MTDLTEPRYSPMKTRSLIDRLTNQEDLNFLLTNRVPRIALTRFM
                     GWFSKIEHPLVCRLSIAIWKLFADLDLSEARHTEFRSLHDCFTRQLKDGARPVDPDPA
                     VLTSPCDAIVGACGPIAGTELIQAKGFPYTLLDLLADPALVDTYRDGSYVTLRLKSSM
                     YHRFHAPADCRVEHTTYISGDVWNVNPVALKRVEKLYCKNERAVIRTRLAADGAPITL
                     VPVAAILVASMRLHWLDVLLHLRYRGPNEMPVRADFRKGQEMGWFEHGSTIIVFAPKG
                     FTLVDGIASGHAIRMGHALLRRPGH"
     gene            5089..5457
                     /locus_tag="PKX01_15525"
     CDS             5089..5457
                     /locus_tag="PKX01_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77594.1"
                     /translation="MDFDELHERACINLRLLLPQVQDPTRLVDECLLSARKSFRDDFI
                     LSLSPEAWARISLVYISTFLDSGVDVPVGDIVAAVVSDSIHLRRIDLLAQQVGQRRAE
                     DKTTRPSNGANSACLKVVKS"
     gene            complement(5498..6283)
                     /gene="yaaA"
                     /locus_tag="PKX01_15530"
     CDS             complement(5498..6283)
                     /gene="yaaA"
                     /locus_tag="PKX01_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HMZ77595.1"
                     /translation="MLFVISPAKALDFDTPPTTPRFTEPDFLDDAAELIAGLRTLSHA
                     QVAELMDLSDALAALNVARYEAWARPFSPQNAKPALLAFDGDVYDGLGAASLREAELD
                     YAQAHLRILSGLYGVLKPLDLMQPYRLEMGTRYANGRGKDLYAFWGDRITEALNACLA
                     AEAAAGRAPVLVNLASEEYFKSVRPKKLKGRLLNVAFEDWKNGRYRIISFYAKRARGL
                     MARHAIRLGAQDVEQLKTFDAAGYAFAPEASDDGKWVFRRRVA"
BASE COUNT         1082 a         2419 c         1813 g          952 t
ORIGIN      
        1 cgcttctaca cccggctcga ctccatgagc cgcatcgtct ccgtcaccca gaaggccgcc
       61 gccggcgcca agcgcatctt cgacatcctc gaccacgtct ccagcgtgcc cgagcccgtc
      121 aatccccagc acctgcccaa ggtcaccggc gccatcgaag tgcgcaacgt gggcttccgc
      181 tacggcaacc gcaccatcct gcgcaatgtg gacctgtcca tccagcccgg cgaaatgatc
      241 ggcctggtgg gtcactccgg ctccggcaag agcactctgg tgaacctcat ctgccgcttc
      301 tacgacgtga ccgagggctc catccgcatc gaaggcgtgg acctgcgcgc cgtgcccgtg
      361 gccgaatacc gccagaacat cggcctggtg ctccaggaac ccttcctgtt cttcggcacc
      421 atcgccgaca acatcgccta cggcaagccc gacgccaccc gcgaagagat catcgccgcc
      481 gcccgcgccg cccacgccca cgacttcatc atgcgcctgc ccttcggcta cgactccctg
      541 gtgggcgagc gcggccaggc cctgtccggc ggcgagcgcc agcgcatctc catcgcccgc
      601 gccctgctca tcgacccgcg catcctgatc ctcgacgaag ccacctcggc ggtggatacc
      661 gagaccgaga tggagatcca ggccgccctc gaaaacctca tccgcggccg caccaccctc
      721 gccatcgccc accgcctgtc caccctgcgc aaggccgacc gcctggtggt gctggaccgc
      781 ggccagatcg tcgagatcgg caaccacgac accctgatgg acaagcaggg cgcctacttc
      841 cgcctctacg aagcccagac ccgcaagctg gacgacgacg ccctcggcgg cggcaccccg
      901 ctgcccgccg ccgcccaccg ccagcacgcc caggcctgac gcggccgcca aggaatcccc
      961 atgtcccagc cccccctgaa cctggcccgc aaccccttcg gccagctcgt cttcaccgcc
     1021 gccgacggca cgccccacac ggtcgtcccc gtgcgcgcct tccccatcgc cgcgcccgcc
     1081 gagaacatcg gcctgatcaa cccggaaggc gaggaagtgg cctgggtgga ccgcctcgcc
     1141 gacctgcccg acgccccgcg ccgcctcatc gaggacgagc tcgccagtcg cgaattcatg
     1201 cccgtcatcc agcgcatccg ccactgctcc agctttgcca cccccagcac ctgggaactc
     1261 gacaccgacc gcggccccgc caccctggtg ctcaagggcg aagaagacat ccgccgcctc
     1321 aagtccccgt ccctgctcat cgccgacagc cgcggcatcc acttcctggt acgcgaccgc
     1381 tacgctctcg acgcccacag caagcggatc ctggatcgct tcctgtaagc cccggggnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc ccaccatcac cgttccgtaa
     1561 catctccgca gccacggagt cacacaactt tcttagtctc gccttgaaag cttttcactt
     1621 tcacaacccc gggagactcc atgaaaaatt ttctgcgtgc ccttgaggca cagcgttggg
     1681 acgaccaccg cttctaccat cacagccgca tcaaccagac gctgcacttc atcagcgcga
     1741 tcagcttcgt cgtggcctac tgcctgatct tcaccgaccc ggtcaaggcc gccctgatcg
     1801 gctggctggt gtccatggtc acgcgccagt ccggccactt cttcttcgag cccctgggct
     1861 acgacgaaga gaaccagtgc accaacgaat acaaggaagc cgtcaaggtc ggctacaacc
     1921 tgcagcgcaa ggtcgtgctg ctgagcctgt gggccgccac gccggtcgtg ctgtacatgg
     1981 acccgacctg cttcggcctc ttcgacgccc atgtagacat ggacggctac atcgaccacc
     2041 tgggcaagct gtggctgagc ctgggcgtgg ccggcctgct cttccgcacg gtgcacctgt
     2101 tcttcctgaa ggacgtgcag accgggctgg tgtggctgac caagatcctc accgacccct
     2161 accacgacat caagctctac caccgggccc cgctggagct gctgcgcgaa ctccgcgccc
     2221 gccgccagac cgcctgatcc cttcgtcgcc tcaccgcgcc acgccgggtt tgccgggtgg
     2281 cgcggtgtcg tttccagcgc cccatgcccg ccatcgactg cccggcccac aaaaaaggcg
     2341 gaacgcacgt ccgtgccgtt ccgccttgcc gcgtggaccg ctcagcccag gaagcgcttg
     2401 cgcagcatct ccttgaggat gcgccggcgg atgtccttgt tggtcgtccg gccatcgtgc
     2461 caccaccagc cgtccgcctc catctgcttc gccgccgcca cgaagcgttc ggccacggcc
     2521 tcgaagtccg cgtcggagta gttcaggctg aagatgaagc gcccgctgcc cacccacgac
     2581 agggccaggc cctcggcccg caggtagtac tggagcatcc agttgtagcg gctcgggcgg
     2641 gtgtagaaca ccgtccacac cgactgcatg ttcgccacct gcactggcag gccggcctcc
     2701 agcagacggg cgttgaggcg cgcggcgcgg ccgttccagg tgtcgtccag gccggcgtag
     2761 accgccttca cctcgggcgt ctccaggcgc gccaggaatt ccgccatcgc ccccatcacg
     2821 tgggggtggg aattgaaggt gccgcgggcg aagcagatgt ccgccggcgc atcgtcgcgg
     2881 aagcgcttca tcagatcgcg ccggccgcac accacgccca ccggcaggcc gccgcccagg
     2941 gtcttgccgt aggtgaccat gtccgcctgc acgccgaagt attcctgcgc gccgccggcc
     3001 gccaggcgga agcccaggaa gacctcgtcg aagatcagca cgatgccccg ctcggtgcac
     3061 acctggcgca gttccttgag ccaggccgtg taggccgccc ggtcgaagcc ggcgcggcgc
     3121 gagctgtcca ccagcgacga atcccccggc gccgcggcgt tgggatggag tccctgcagc
     3181 ggattgatca gcacgcaggc gatgtccttg cgccggcgca gcacgcgcag ggcgtccgga
     3241 tgcatgtcgc gcagggtgta ggtgtgctcg gccgcgacgg gattgcctac gcccggctgc
     3301 acgtcgcccc accagccgtg gtagctgccg cagaagcgca ccagatgatt gcgccgggtg
     3361 tggtagcggg ccaggcgcac ggcctgcatc accgcctcgg tgccggacat gtggaaggac
     3421 acctcgtcca gcccggaaac ggccttcagg cggcgcacat tctccagcag caccggatgg
     3481 taagagccca gcaccggccc cagcgctgcc acccgttcgg caccgcgggc catgcaggtc
     3541 ttgtagaagt cgtagccgaa gacattcacc ccgtagctgc cggccaggtc gtagaagacg
     3601 ttgccgtccg cgtccgtcac cgtcacgccc gccgaggcgg tgtagaagct gccgcccttc
     3661 aggtgttccc gcaccaggcg gctgtactgg aacggcaccc ggtaggcgcc ggtgaattgc
     3721 aggtccgaca cgccctccgc cgcctcggcg gtgagcgccg cgctgcgggc gaagcgcgcg
     3781 cggtacagct cagccagacg gaagaagccc tcgcgacggc gcgccaccac ctcggccggg
     3841 gcatcgtcgg aattgaagaa gcccgcttcg tcgtactcgt agaacggcag cagacgcgcc
     3901 acccggcggg acatcttcgc gtgtccggcc agggacggat gcttgccctt cgacagctcc
     3961 aggcggcgct tgatcttgag cgccagaccc ggcaaggcca gcgcccccag cccggcccac
     4021 accaatgcat cttccacgga tttcctttcg gcgactgaaa gatgactgat ttaaccgaac
     4081 cccgttacag ccccatgaaa acccgctccc tcatcgaccg gctcacgaac caggaagacc
     4141 tcaacttcct cctcaccaac cgcgtcccgc gcatcgccct gacccgcttc atgggctggt
     4201 tcagcaagat cgagcacccg ctcgtgtgcc gcctctccat cgccatctgg aagctcttcg
     4261 ccgacctgga cctgtccgaa gcccgccaca cggaattccg cagcctgcac gactgcttca
     4321 cccgccagct caaggacggc gcccgccccg tcgatcccga ccccgcggtg ctcaccagcc
     4381 cctgcgacgc catcgtcggc gcctgcggcc ccatcgccgg caccgagctg atccaggcca
     4441 agggcttccc ctacacgctc ctggatctcc tcgccgatcc cgccctggtg gacacctacc
     4501 gggacggcag ctacgtcacc ctgcgcctca aatccagcat gtaccaccgc ttccacgccc
     4561 ccgccgactg ccgcgtggag cacaccacct acatctccgg cgacgtgtgg aacgtcaatc
     4621 ccgtcgccct caagcgcgtc gaaaagctct actgcaaaaa cgagcgggcc gtcatccgca
     4681 cccgcctcgc tgccgacggc gcgcccatca ccctggtgcc ggtggccgcc atcctggtgg
     4741 ccagcatgcg cctgcactgg ctggatgtgc tcctccacct gcgctaccgc ggccccaacg
     4801 agatgcccgt ccgcgccgac ttccgcaagg gccaggagat gggctggttc gagcacggct
     4861 ccaccatcat cgtgttcgcc cccaagggct tcacgctggt ggacggcatc gcctccggcc
     4921 acgccatccg catgggccac gccctgctgc gccggccggg ccactgaacc ctggcgcagg
     4981 ccccgcagac ccgcgccctc tggacgacaa cggcgcgggc aattatcgtt cgactgtgga
     5041 aggcccggcc acgcccgggt ccggatggca accgtccccg gaggcgccat ggacttcgac
     5101 gaactgcacg aacgtgcctg catcaacctg cgcctgttgt tgccccaggt gcaggatccc
     5161 acccgcctcg tggacgaatg tctgctcagc gcgcgcaaat ccttccgcga cgacttcatc
     5221 ctcagcctca gcccggaagc ctgggcgcgg atctccctgg tgtatatctc cacctttctg
     5281 gattccggcg tggacgtgcc ggtgggcgac atcgtcgccg cggtcgtcag cgactccatc
     5341 cacctgcgac gcatcgacct gctggcccag caagtcggtc agcgccgcgc cgaagacaag
     5401 accacccgcc catccaatgg cgccaacagc gcatgtctga aggtggtcaa atcctgagga
     5461 aggcagcgcc cgcagacgtc cgcgccccgg gcgcccgcta cgccacccga cgacggaaca
     5521 cccacttccc gtcgtccgac gcctccggcg caaaggcata acccgccgca tcgaaggtct
     5581 tcaactgctc cacatcctgc gcccccaggc ggatcgcgtg ccgcgccatc aaaccgcggg
     5641 cccgcttggc atagaagctg atgatgcggt aacgcccgtt cttccagtcc tcaaaggcca
     5701 cgttgagcaa gcgccccttc agcttcttcg gccgcaccga cttgaaatac tcctccgacg
     5761 ccagattcac cagcaccggc gcccgcccgg ccgccgcctc cgccgccaga caggcattca
     5821 aggcctccgt gatgcggtcg ccccagaagg catacagatc cttgccccgc ccattggcgt
     5881 agcgcgtccc catctccagc cgataaggct gcatcagatc cagcggcttc aacaccccgt
     5941 acagcccgga caggatgcgc agatgcgcct gggcatagtc cagctccgcc tccctcaggc
     6001 tggccgcccc aagcccgtca tacacatccc cgtcgaaggc cagcaaggcc ggcttcgcgt
     6061 tctgcggcga aaacggccgc gcccacgcct cataacgcgc cacattcaag gccgccaacg
     6121 catccgacag atccatcagt tccgccacct gggcatggga caaggtccgc aaacccgcaa
     6181 tcagctccgc cgcatcgtcc agaaaatccg gctccgtgaa gcgcggcgtg gtgggcggcg
     6241 tgtcgaagtc gagggctttg gcgggggaga tgacgaagag catggcgggt gcgacagggc
     6301 gtgtgaggtg tgtgacaggg acgcgggagg atagcaaagg gcgtgccgtg gggggagcgg
     6361 gttacg
//
LOCUS       DAOQPA010000157         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7071_length_6364_cov_14.744809, whole genome shotgun sequence.
ACCESSION   DAOQPA010000157 DAOQPA010000000
VERSION     DAOQPA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_6364_cov_14.744809"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKX01_15535"
     CDS             <1..542
                     /locus_tag="PKX01_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ77596.1"
                     /translation="MLRVPGSRGDLLGARAEATDREADMERAARRPQVGVNAAVMHED
                     NRYRASQDVAQVAVGVQWELFDGGIQRQRADAAAARARAARERQADTATLIELEVRRE
                     WLAVEEAAQRLATTGAAEAQADENLRVARDRYRNGVGTHTEVLDAEALRALTDSNHLN
                     AHYDLHGARYRLKKAVGEL"
     gene            611..1678
                     /locus_tag="PKX01_15540"
     CDS             611..1678
                     /locus_tag="PKX01_15540"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77597.1"
                     /translation="MNILTRRIGGAVAVVAVLAAGGWAWHEWAGSAALPEGLIQANGR
                     IEGDRTTVASKLAGRIAALKVREGDSVQAGQVLARLEDTQLAARQQQADAAVATLQAQ
                     VAAAQAQLAVARREVPLAEASADAQLAKARAADAQAAKDAARFDELAERGTVEVRRAE
                     QMRLVRSSAAADLRQAEQALASAHLGHDKVKARENDVAALEAGLHQAQAAAKEVASLI
                     DDLSLRAPVGGVVLNRLRESGEMVAAGTPVFEIVDLDRLYLKVYVPEKLIGQLRLGLP
                     ARVYSDAFPAQAFAATTRYIASRAEFTPKEVQTPDERVKLTYAVKLYLDANPEHRLAP
                     GVPADAVIRWKDEVPWQAPRW"
     gene            1686..3662
                     /locus_tag="PKX01_15545"
     CDS             1686..3662
                     /locus_tag="PKX01_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ77598.1"
                     /translation="MNAPAIRVEGFVKRYGRRVAADGLTLTVQPGEIYGLVGADGAGK
                     SSLMKAVAGVLAYEGGRVEVFGLPVDSERAAERVKQRIGFLPQGLGLNLYPDLSVEEN
                     VDFFGRLRGVSAAQLAERKTLLLAMTRLDAFRARPMRQLSGGMKQKLGLVCTLIHEPD
                     LVILDEPTTGVDPVSRRDFWAILGRLLEEKGITALVSTAYLDEADRFHRVSLFHEGKV
                     MGEGTPAALVATVPGTLALFRAEPQAEALPRLKARFPQTEAMGAWFRVFAEGLDADAA
                     RGAVAACLDIAPAATTTGTAAPTIDLRDWFAREPELEDVFVALLRRRGTQLDSRVEGV
                     TRRAVAPGELAIEARELTRVFGEFRAADAVSFAVPQGEIFGLLGANGAGKTTVIKMLT
                     GILKPSSGAGRVAGADMRTAGLAIKERIGYMSQAFSLYHDLSVVENIRLYAGIYGLDD
                     RQAQRRTDWVIGMAGLAGFEHDGAGRLPMGLRQRLALGCALVHEPRVLFLDEPTSGVD
                     PIGRRMFWDILFHLSRREGVAILVTTHYMSEAEHCDHLALMYAGRVVADASPEAMKAA
                     IAEESGRLLELRVDRPVPAQAALKAAGYGSAALHGRAVHLLSRDVDGDLARLPGVLAA
                     AGVQVQAAGLRPLSMEDVFVQRITALEEAARRAP"
     gene            3659..4813
                     /locus_tag="PKX01_15550"
     CDS             3659..4813
                     /locus_tag="PKX01_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77599.1"
                     /translation="MNGRRIVSLAWKEWREIVRDRLFFALAFFVPTLLMVLFGFGLTL
                     DVENLPFAVVDQDRSAASRDYAYRFVSSRYFDFKGQVEDVRQLDRLLADNSIRLALVI
                     PPRFGEHLARGEPAPVQVLIDGTFPMRAQTTQGYVAAIHSAVSLDSMAQALSALRGIP
                     LAEAQTRVQPVKLEVRYLYNQSVKSIWSLAPKLIMLIMMLSPPFLTALGVVREKESGS
                     IFNIYASTVSPGEFLIGKLAPYVLISFINGLILWALAIWLYGTPFKGDPLFFALALLL
                     YVVCTTGIGLVISMLVQTQVAAMIVASIVSIIPAVLYSGMLLPLSSLAPSALAIAHAL
                     PPMYFTNIVVGSFLKGVGWGALWGDVLVLAAYALGLFGIGYLLFTKRPKS"
     gene            4810..5961
                     /locus_tag="PKX01_15555"
     CDS             4810..5961
                     /locus_tag="PKX01_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ77600.1"
                     /translation="MNGLFSAAGLRRLRVMTRKELIQLFRDVALMVFFVYAFTGDIYI
                     AASGVSMQLKNAALAVLDGDASFASRELSSRFQPPYFRVAGAAGTPAQSIRHLDNADV
                     MALLDIPPRFEETLARGGQAAVQLQVDTSNSVLGFLAASYGAQIVGKYALETAALREG
                     LGSGGVAGPTLAAETRVWYNPNQNDAWFMGISELLTVITLFSILLPAAAMVREKERGT
                     IEQLMVTPLSAFQILCPKVVAMTLVILAGTAIALFGILVPCFDVPMRGSLVSFFLITA
                     LYVFTTAGLGLLAATVARNLAQVGMLTVVILMPMLLLSGAWTPIEAMPEWMHLLAWIS
                     PLHYFLDAAYGILLKGAGLDLLWPSVLSMAALGVSVAALGMGRFGRQFG"
BASE COUNT          902 a         2309 c         2163 g          990 t
ORIGIN      
        1 acatgctgcg ggtgccggga tcgcggggcg acctgctggg cgcccgcgcc gaggccacgg
       61 atcgcgaggc cgacatggaa cgcgccgccc gccggcccca ggtgggcgtc aatgcggcgg
      121 tgatgcacga ggacaaccgc taccgggcga gtcaggacgt cgcccaggtg gccgtgggcg
      181 tgcagtggga attgttcgac ggcggcatcc agcgtcagcg cgccgatgcg gccgcggcgc
      241 gggcccgtgc cgcgcgggaa cgtcaggccg acaccgccac gctgatcgag ctggaagtcc
      301 ggcgcgaatg gctggccgtg gaagaggccg cccagcgcct cgccaccacg ggcgccgccg
      361 aggcccaggc cgacgagaac ctgcgcgtgg cccgcgaccg ctatcgcaac ggcgtcggca
      421 cccacaccga ggtgctggac gccgaggcct tgcgcgccct caccgacagt aatcacctca
      481 acgcccatta cgacctgcat ggggcccgct accgcctgaa gaaggcggtc ggagaactct
      541 gaacggcgcc ccgccggctc ccttgggggg cgctgatgcg ggccggtctt ttgactaagg
      601 aatcccttcc atgaacatcc tgacacgccg catcggcggt gccgtagccg tggtggccgt
      661 gctggcggcc ggcgggtggg cctggcacga atgggccggc agcgcggccc tgcccgaggg
      721 cctgatccag gccaacgggc gcatcgaggg cgatcgcacc acggtggcga gcaagctggc
      781 cgggcgcatc gcggcgctca aggtgcgcga aggcgacagc gtgcaggccg ggcaggtgct
      841 ggcccgcctc gaagacaccc agctcgccgc ccgtcagcag caggcggacg ccgctgtggc
      901 cacgctccag gcgcaagtgg cggcggccca ggcccagctg gccgtggcgc ggcgcgaagt
      961 gcccctcgcc gaggccagcg ccgacgccca gctcgccaag gcgcgggccg ccgacgccca
     1021 ggccgccaag gacgccgccc gcttcgacga actggccgag cgcggcaccg tggaggttcg
     1081 ccgcgccgag cagatgcgcc tggtgcgcag cagcgccgcg gccgatctgc gccaggccga
     1141 gcaggccctg gcgtcggccc atctgggcca cgacaaggtc aaggcgcggg agaacgacgt
     1201 ggctgcgctg gaagccggtc tgcatcaggc ccaggcggcg gcgaaggagg tggccagcct
     1261 gatcgacgac ctgtccctgc gggcgccggt gggcggcgtg gtcctcaacc gcctgcggga
     1321 atccggcgag atggtggcgg cgggcacgcc ggtgttcgag atcgtcgatc tggaccggct
     1381 ctatctcaag gtgtacgtgc ccgagaagct catcggccag cttcgcctgg ggctgccggc
     1441 gcgagtgtat tccgacgctt tccctgctca ggcctttgcc gccacgacgc gctacatcgc
     1501 ctcccgcgcc gagttcacgc ccaaggaagt gcagacgccg gacgagcggg tcaagctcac
     1561 ctacgcggtc aagctctacc tggacgccaa tccggagcat cgcctggcgc cgggcgtgcc
     1621 ggccgacgcg gtgatccgct ggaaggacga ggtgccctgg caggcgccgc gctggtgagt
     1681 cggtcatgaa cgccccggcg atccgcgtcg aaggcttcgt caagcgctac ggccggcgcg
     1741 tggccgccga cgggctcacg ctcaccgtgc agcccggcga gatctacggg ctggtgggcg
     1801 cggacggcgc cggtaaatcc agcctgatga aggccgtggc cggcgtgctc gcctacgaag
     1861 gcggccgcgt ggaggtcttc ggcctgccgg tggattccga gcgggcggcg gagcgggtca
     1921 aacagcgcat cggctttctg ccccaggggc tcgggctcaa cctgtatccc gacctgtccg
     1981 tcgaggagaa cgtggatttc ttcggccgcc tgcgcggcgt gagcgccgcc caactggccg
     2041 aacgcaagac gctgctgctg gcgatgaccc gcctggacgc cttccgtgcc cgccccatgc
     2101 gccagctctc cggcggcatg aagcagaagc tcgggctggt gtgcaccctg atccacgagc
     2161 cggacctggt catcctcgac gaacccacca caggcgtgga cccggtgtcg cggcgcgact
     2221 tctgggcgat cctcgggcgc ctgctggagg agaagggcat caccgcgctg gtgtccacgg
     2281 cctacctgga cgaggcggac cgcttccacc gggtgtccct gttccacgaa ggcaaggtga
     2341 tgggcgaagg cacgcccgcg gcgctggtag ccacggtgcc cggcaccctg gccctgttcc
     2401 gcgccgaacc ccaggccgag gccctgccgc ggctcaaggc ccgcttcccc cagacggagg
     2461 cgatgggcgc ctggtttcgc gtgttcgccg aggggctgga cgcggacgcg gcccgcggcg
     2521 ccgtggcggc ctgcctggac atcgcgccgg ccgcgacgac gacgggcacc gctgcgccga
     2581 ccatcgacct ccgcgactgg ttcgcccgcg agcccgaact ggaggacgtc ttcgtcgccc
     2641 tgctgcgccg ccgcggtacg cagctcgaca gccgcgtcga aggggtcacg cgccgcgccg
     2701 tcgcgccggg cgaactggcc atcgaagcgc gtgagctgac ccgtgtcttc ggcgaattcc
     2761 gtgccgccga cgcggtgagc tttgccgtgc cccagggcga gatctttggc ctgctggggg
     2821 ccaacggcgc gggcaagacc acggtcatca agatgctcac cggcatcctc aaaccctcgt
     2881 ccggcgcggg ccgggtggcg ggcgccgaca tgcgcacggc gggcctcgcc atcaaggaac
     2941 gcatcggcta catgagccag gccttttccc tgtatcacga cctctcggtg gtggagaaca
     3001 tccgcctcta cgccggcatc tacgggctcg acgaccgcca ggcgcagcgc cgcaccgact
     3061 gggtgatcgg catggccggg ctggccggct tcgagcacga cggggccggg cgcctgccca
     3121 tggggctgcg ccagcgcctc gccttgggct gcgccctggt ccatgagccg cgggtgctct
     3181 ttctcgacga gcccacatcg ggcgtggatc ccatcggccg gcgcatgttc tgggacatcc
     3241 tcttccacct gtcgcggcgc gagggcgtgg ccatcctggt gaccacccac tacatgagcg
     3301 aggcggagca ctgcgaccac ctggccctga tgtacgccgg ccgcgtggtg gcggacgcct
     3361 cgccggaagc catgaaggcc gccatcgccg aagaatccgg ccgcctgctg gagctgcgcg
     3421 tggatcggcc ggtgccggcc caggcggcct tgaaggcggc gggctatggc agcgcggccc
     3481 tgcacgggcg cgccgtgcac ctgctgtcgc gggatgtgga cggcgatctg gcgcgtctgc
     3541 cgggcgtgct ggccgcggcg ggcgtgcagg tgcaggcggc cggcctgcgt cccttgtcca
     3601 tggaggacgt cttcgtgcag cgcatcaccg cgctggaaga ggccgcacgg agggcgccat
     3661 gaacggccgc cgcatcgtct ccctggcctg gaaggaatgg cgcgagatcg tccgcgaccg
     3721 gctgttcttc gccctggcct tcttcgtgcc gaccctgctc atggtgctgt tcggcttcgg
     3781 cctcaccctg gacgtggaga acctgccctt cgccgtggtg gaccaggacc gcagcgcggc
     3841 gagccgggac tacgcctacc gcttcgtctc gtcccgctat ttcgacttca agggccaggt
     3901 ggaggacgtg cgccagctcg accgcctgct cgccgacaac agcatccgcc tggccctggt
     3961 gattcccccg cgctttggcg aacatctggc acggggcgag ccagcgccgg tccaggtgct
     4021 catcgacggc accttcccga tgcgcgccca gaccacccag ggctatgtgg ctgccatcca
     4081 tagcgcggtc agcctggaca gcatggccca ggccctgtcg gccctgcggg gcattcccct
     4141 ggccgaggcc cagacccgcg tgcagccggt gaagctggag gtgcgctacc tctacaacca
     4201 gagcgtgaag agcatctggt ccctggcgcc caagctgatc atgctcatca tgatgctgtc
     4261 gccgcccttc ctcaccgccc tgggcgtggt gcgcgagaag gaatccggct ccatcttcaa
     4321 catctacgcg tccaccgtgt cgccggggga attcctgatc ggcaagctgg cgccctacgt
     4381 gctcatctcc ttcatcaacg ggctcatcct gtgggccctg gcgatctggc tgtacggcac
     4441 ccccttcaag ggcgatcccc tgttcttcgc gctggcgttg ctgctctacg tggtgtgcac
     4501 caccggcatc gggctggtca tttccatgct ggtgcagacc caggtggcgg ccatgatcgt
     4561 cgccagcatc gtcagcatca tccccgcggt gctctactcc ggcatgctgc tgcccctgtc
     4621 gtccctggcc cccagcgcgc tggccattgc ccatgccctg ccgcccatgt acttcaccaa
     4681 catcgtggtg ggcagcttcc tcaagggcgt gggctggggc gccctgtggg gcgacgtgct
     4741 ggtgctggcc gcctacgccc tgggcctgtt cggcatcggc tacctgctgt tcaccaagcg
     4801 gcccaaatca tgaacggact cttctcggcg gccggcctgc gccgcctgcg ggtgatgacc
     4861 cgcaaggagc tgatccagct cttccgcgac gtggccctga tggtcttctt cgtgtatgcc
     4921 tttaccggcg acatctacat cgccgcgtcc ggcgtctcca tgcagctcaa gaacgccgcg
     4981 ctggcggtgc tcgacggcga cgccagtttt gcctcgcggg aactgagcag ccgcttccag
     5041 ccgccgtatt tccgcgtggc cggtgcggcc ggcacgccgg cccagtccat ccgccacctg
     5101 gacaacgccg acgtgatggc cctcctcgac attccgccgc gcttcgagga aaccctcgcc
     5161 cgcggcggcc aggccgccgt gcaactgcag gtggacactt ccaattccgt gctcggcttc
     5221 ctcgccgcca gctacggcgc ccagatcgtc ggcaagtacg ccctggagac cgccgccctg
     5281 cgcgaaggcc tgggcagcgg cggcgtggcc ggccccaccc tcgccgcgga gacgcgggtc
     5341 tggtacaacc ccaatcagaa cgacgcgtgg ttcatgggca tctccgaact gctcaccgtg
     5401 atcaccctgt tctccatcct gctgccggcg gcggccatgg tgcgggagaa ggagcggggc
     5461 accatcgagc aactgatggt cacccccctg agcgccttcc agatcctgtg ccccaaggtg
     5521 gtggccatga ccctggtgat cctcgccggc accgccatcg ccctgttcgg catcctcgtg
     5581 ccctgtttcg acgtgcccat gcgcggcagc ctcgtcagct tcttcctcat caccgccctc
     5641 tacgtcttca ccaccgccgg cctgggcctg ctggccgcca ccgtcgcccg caacctggcc
     5701 caggtgggca tgctcaccgt ggtgatcctg atgcccatgc tcctgctctc cggcgcatgg
     5761 acccccatcg aggccatgcc cgaatggatg cacctcctgg cgtggatctc cccgctgcac
     5821 tacttcctcg acgccgccta cggcatcctc ctcaagggcg ccgggctgga cctgctgtgg
     5881 ccctcggtcc tgtccatggc cgccctgggg gtgagcgtcg cggccctggg catgggacgc
     5941 ttcggacgcc agttcggctg aagcgcacgc cgcgatacgg cgggggaggg ggcggcacgg
     6001 gcgccctcgc ccgcatggcg gccgatcccc acgcaccagc caaagcccac gtcctccgca
     6061 caccggccgg catgctcgcc gcggacggcg gggacgccct gatcgcgccg cccgtcggcc
     6121 ccccaatgcg ggacgcgcat ccgacggagc gtgccgcccc aatgccgcgg cgtgcccgcc
     6181 tgctgcggcg gcacgacgtt ccccgcgcgg gtattccttt ccatcatccc gccggaacgc
     6241 cctctcgaca gccacgcctt gtctgcgcca atggcgtttg ggtgcaatca atagtgaata
     6301 tctataaatc aatgatgcga gtttgatcac tccctatggt gaaagcctca cgatagtctg
     6361 tgag
//
LOCUS       DAOQPA010000158         6359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7081_length_6359_cov_16.483344, whole genome shotgun sequence.
ACCESSION   DAOQPA010000158 DAOQPA010000000
VERSION     DAOQPA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_6359_cov_16.483344"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKX01_15560"
     CDS             <1..313
                     /locus_tag="PKX01_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77601.1"
                     /translation="LAHILYSRAQIRLQRGDHHDSGLHAIYEDLARAYAFLVKLGRPD
                     GIGTAGALLAQVLAMEGSRSEALQVLETAEAAWRKIGQMQQVARVAELRQTIEGRGDG
                     Q"
     gene            complement(736..2298)
                     /locus_tag="PKX01_15565"
     CDS             complement(736..2298)
                     /locus_tag="PKX01_15565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ77602.1"
                     /translation="MTAAPHSSGPLQRSHYAALLAVIATVLTAYRILVIGHLGIDLYV
                     DEAYYWGWSKALDWGYFSKPPLIAALIAASTALFGDGLLAIKLPSLLLYPATAAALFV
                     LGERLHSVRAGFWAGLIFMTMPLVSALGLFVSTDAPLLLCWALALIALLKAVDAEQGW
                     GAWLAWGAAVGVGLMAKYTMAAFLPSALLLMLLEPAGRRWLRRPQPWIALLLALAIFS
                     PNLYWNWIHHFPTFRHTAEITEVGHRGWHPGELGEFIGAQWLSLGPLMGILLAWALAR
                     SRRIGQDRAGRVLLIFILPLLTLVSLQALTGRANGNWAAPIFVAACVLIPAYFLAERR
                     RWLVAGVALNLVGSLFVYHWPDVARLADIPLTAKTDPYKRARGWTRLADGVAPYLAAH
                     PDAILVGEDREITAHLVYRLHPRDYAAWNPGGRPPIDHYQLVTHLDDQLGRDILYVGN
                     VPELPGMEPYYAAIEPLGKVVVPVHTDFRRTVYVFLLKGFKGYKPSAASAPTIAPVPT
                     PESSTPPVVSRP"
     gene            complement(2295..3863)
                     /locus_tag="PKX01_15570"
     CDS             complement(2295..3863)
                     /locus_tag="PKX01_15570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ77603.1"
                     /translation="MNGLASGRLAGSTLGRLILALPLFAFLLRLGGAPLFDVDEGAFS
                     EATREMFERGDFLFTYLNGEPRYDKPILIYWLQALGYLIFGPSEWAFRLPSALAAIAW
                     SYATWLFARRRIGEDAALVALAVAASSLGPFAIGRAATADGLLNCLLALSLFDLWRHL
                     ESGERAPLLRAYLWIGLGALTKGPVALIVPGAVTCLYCLSRGEGLRWLRMIAHPAGWA
                     ILLLLIAPWYAYALHRHGQDFINGFLLKHNVQRFSGALEGHGGSAAYYLVAVPLLLLP
                     WSGLLGVALSRLRSDVAHPLRRFLWIWFGFVLVFFSLSGTKLPHYVLYGCTPLFVLIG
                     LHADGARRIWPHLLVPGLLMLFCIALPGVLDHLAHGTAGDGYYRAQFSRALAEAGPLY
                     YGLTAGALLLWLAFVLFIRVPLIHKLAVTAILQVGLLAGVVVPYAGELAQGPVKAAGR
                     VARDLHEPVIFWNFSTVPSFSVYRQAVTHLAEPAPGELALTRIDRLPPDVPVETLFQR
                     GGVVLVRRLPAPKP"
     gene            complement(3874..4932)
                     /locus_tag="PKX01_15575"
     CDS             complement(3874..4932)
                     /locus_tag="PKX01_15575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ77604.1"
                     /translation="MTDTALSSPLPAAAPDIPADLPPHRLSVVVPLYNEEDNVDPLLE
                     RIHLALGPYPYPWEAVIVDDGSSDNTVPRLEAAAKRYGPHVRIVALMRNFKQTAAMQA
                     GLDAARGDVIVTMDGDLQNDPIDIPRMVGRLLREDLDLVAGWRKNRKDGMILRKIPSK
                     IANRLIARITGVHLQDYGCSLKAFRATAIKNVRLYGEMHRFIPAWLATVTTPRKIAQE
                     VVTHHARVFGQSKYGISRTFRVILDLVFVYFFMRFRTRPGHFFGGIGLGLGALGVLIL
                     GYLSLLKLFTGASIGTRPMFFGGFFFVIAGVQMVTSGVLAELLTRVYFESGTSLAYVA
                     RETAPLADEQGWSGGPKA"
     gene            complement(4970..5941)
                     /locus_tag="PKX01_15580"
     CDS             complement(4970..5941)
                     /locus_tag="PKX01_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ77605.1"
                     /translation="MTLKRLFAVALSLALIGWLAADGRWRGVGEVFARVPLSVLAVSA
                     LGFGLSYSLRALRVWDEFRRHASGRFGACLRIVLIHSAMINVLPFRGGEAAFPLLLRQ
                     TFGVPLPRALASLFWFRLQDAFAVMGVAALVWPGLPTLLKLAGAVSVFALAWWLPRWA
                     RAPHAWADHGGLTAKLAKLRDAFAESTRHARFGWLWTLTNWSVKLAAQAWLLAALIPA
                     ALDVGAAGALGAELAAILPVQGVAGFGTYEAGAAAALLPSGVALGTGLQVALALHLFV
                     IAVSVSGGALAWLVPVAAPAEASAPDADPSADVQSASSAADSTRPAP"
     gene            complement(5938..>6359)
                     /locus_tag="PKX01_15585"
     CDS             complement(5938..>6359)
                     /locus_tag="PKX01_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ77606.1"
                     /translation="YVDAFPSGHATTAFVVAAAVLLAWPDAPRRALTWGLLLLAAGLV
                     AFSRIAVGAHWPADVAVGACGGWLCGALGAALTARLRFWQKDAAIRLMAGIALIASLA
                     LLLVNVGYPEARLYQIGLGAWGIGGAAAALMRERSAA"
BASE COUNT         1088 a         1946 c         2556 g          769 t
ORIGIN      
        1 tctggcccac attctgtatt cccgggccca gatccgcctg caaaggggcg accaccacga
       61 ctcgggcctg catgccattt acgaagacct ggcccgtgca tacgccttcc ttgtgaaact
      121 ggggcgtccg gacggcatcg ggaccgcggg agccctgctg gcccaggtgc tggccatgga
      181 gggatcgcgg agcgaagcgc tccaggtgct ggagaccgcc gaagccgcct ggcggaagat
      241 cgggcagatg cagcaggtgg caagagtggc ggaactccgc cagacgatcg aggggcgcgg
      301 ggacgggcag tagggatacg cccgtgccca gcgttgtcgg gcagcgcgac gcactttgtg
      361 gcgccgtcaa cacggtgtgt ggaccggtcc acggatgatg atgatcggtg cacacacact
      421 gtcgaccggt cgacggacga tgtggacggg tcgacagtgt gtgtggacgg gtcaacagac
      481 gatgtcgacc ggtccacagt cttgtaagcg accgctacat tgaggattga tagccctttc
      541 cattaggaag aggtctctta caaaggtaaa gggctatcaa cagccttggt gtccacgtct
      601 acagggtctg tggacgtctg cgcagacctt gcagggcggt ccgcagggtg atggatgccc
      661 tgagacatgg catcggccac gccgacagcc gtgttgtccg ggcgcacagg gtctggggag
      721 gcctgcgcag acttgtcagg gccgggacac gaccggtggc gtcgagcttt cgggagtggg
      781 taccggcgcg atggtcggcg cagacgctgc gctcggcttg tagcccttga accccttcag
      841 cagaaacaca tacaccgtgc gacggaagtc cgtgtggacg ggcacgacca ccttgccgag
      901 gggttcgatg gcggcgtagt agggctccat gccggggagt tccggcacgt tgcccacgta
      961 gaggatgtcg cggccgagct ggtcgtccag gtgggtgacg agctggtagt ggtcgatggg
     1021 cgggcggccg ccggggttcc aggcggcgta gtcgcggggg tggaggcggt agaccaggtg
     1081 ggcggtgatc tcccggtctt cgccgacgag gatggcgtcc gggtgggcgg cgaggtaggg
     1141 ggcgacgccg tcggcgaggc gggtccagcc gcgggcgcgc ttgtaggggt cggtcttggc
     1201 ggtgaggggg atgtcggcca ggcgggcgac gtcgggccag tggtagacga agagggagcc
     1261 caccaggttg agggccacgc cggccaccaa ccagcggcgc cgctcggcca ggaagtaggc
     1321 ggggatcagc acgcaggcgg cgacgaagat gggcgcggcc cagttgccgt tggcgcgccc
     1381 ggtgagggcc tggaggctga ccagcgtcag caggggcagg atgaagatga gcagcacgcg
     1441 accggcgcgg tcctgcccga tgcgccggct gcgggccagg gcccaggcca gcaggatgcc
     1501 catcaggggc cccagggaca gccactgggc gccgatgaat tcgcccagtt cgccggggtg
     1561 ccagccccgg tggccgactt cggtgatctc ggcggtgtgg cggaaggtgg ggaagtggtg
     1621 gatccagttc cagtacaggt tggggctgaa gatggccagg gccagcagca gggcgatcca
     1681 cggctggggc cggcgcagcc agcggcgccc ggccggctcc aggagcatca gcagcagggc
     1741 ggagggcagg aaggcggcca tggtgtactt ggccatcagg ccgacgccga ccgcggcacc
     1801 ccaggccagc caggcgcccc agccctgttc cgcgtccacg gccttgagca gggcgatgag
     1861 ggccagggcc cagcacagga gcagcggggc gtcggtggac acgaacaggc ccagggcgga
     1921 gaccaggggc atggtcatga agatcagccc ggcccagaag ccggcgcgca ccgagtggag
     1981 gcgttcgccc agcacgaaga gggccgcggc ggtggccgga tagaggagca gggagggcag
     2041 cttgatggcg agcaggccgt cgccgaagag ggcggtgctg gcggcgatca gggcggcgat
     2101 gagcggtggc ttggagaagt agccccagtc cagggccttg gaccagcccc agtagtaggc
     2161 ctcgtccacg tagaggtcga tgcccaggtg gccgatgacg aggatccggt aggccgtgag
     2221 gacggtggcg atgacggcca gcagggcggc gtagtggctg cgctgcagcg ggccggagga
     2281 atggggtgcg gcggtcatgg cttgggcgcg ggcaggcggc gcaccagcac cacgccgccg
     2341 cgctggaaca gggtctccac gggtacgtcc ggcggcaggc ggtcgatgcg ggtcagggcc
     2401 agctcgccgg gggccggctc ggccaggtgg gtgacggcct ggcgatagac gctgaagctg
     2461 ggcacggtgg agaaattcca gaagatcacc ggctcgtgga gatcccgcgc cacccggccg
     2521 gcggccttga cggggccttg ggccagctcg cccgcgtagg gcaccacgac ccccgccagc
     2581 aggcccacct ggaggatggc ggtgacggcc agcttgtgga tcaggggcac gcggatgaac
     2641 agcacgaagg ccagccacag gagcagggcg ccggccgtga ggccgtagta gagcggtccg
     2701 gcttcggcga gggcgcggct gaactgggcg cggtagtagc cgtcgcccgc cgtgccgtgg
     2761 gcgaggtggt ccagcacgcc gggcagggcg atgcagaaga gcatcagcag acccggcacc
     2821 agcaggtgcg gccagatccg ccgggcgccg tcggcgtgca ggccgatgag cacgaagagg
     2881 ggcgtgcagc cgtagagcac gtagtggggc agcttggtgc cggagaggga gaagaacacc
     2941 agcacgaagc cgaaccagat ccacaggaag cgccgcaggg ggtgcgccac gtcgctgcgc
     3001 aggcggctca gggcgacgcc cagcaggccg ctccagggca gcagcagcag gggcacggcc
     3061 accaggtaat aagcggcgct gccgccgtgg ccctccaggg cgccggagaa gcgctgcacg
     3121 ttgtgcttga ggaggaagcc gttgatgaag tcctggccgt ggcggtgcag ggcgtaggcg
     3181 taccaggggg cgataagcaa aagcaggatc gcccagccgg ccgggtgggc aatcatgcgc
     3241 agccagcgca ggccctcgcc gcgggacagg cagtacaggc aggtgacggc gccggggacg
     3301 atcagcgcga ccggtccctt ggtgagggcg cccaggccga tccacaggta ggcgcgcagc
     3361 aggggcgcgc gttcgccgct ctccaggtgg cgccacaggt cgaagaggga cagggccagc
     3421 aggcagttga gcagcccgtc ggcggtggcg gcgcggccaa tggcgaaggg gcccagggac
     3481 gaggcggcga ccgccagcgc caccagggcc gcgtcttcgc cgatgcggcg gcgggcgaac
     3541 agccaggtgg cgtagctcca ggcgatggcg gccagcgccg aaggcaggcg gaaggcccac
     3601 tcgctggggc cgaagatcag gtagcccagg gcctgcagcc agtagatgag gatgggcttg
     3661 tcgtagcgcg gttcgccgtt gaggtaggtg aacaggaagt cgccgcgctc gaacatctcc
     3721 cgtgtggcct cggagaaagc tccttcgtcc acgtcgaaga ggggcgcgcc ccccaggcgc
     3781 agcaggaagg cgaagagggg cagggcgagg atcaggcggc cgagggtgga cccggcgagg
     3841 cgtccgctgg cgaggccgtt catcgggcgg ggatcaggcc ttggggccgc cggaccagcc
     3901 ctgctcgtcc gccagcggag cggtttcgcg ggccacgtag gcgaggctgg tgccggattc
     3961 gaaatacacc cgggtgagca gttcggccag cacgccggag gtgaccatct gcacgccggc
     4021 gatgacgaag aagaagccgc caaagaacat gggccgggtg ccgatggagg cgccggtgaa
     4081 cagcttgagc aggctcaggt agccgaggat caggacgccc agggcgccga ggcccaggcc
     4141 gatgccgccg aagaagtggc ccggccgggt gcggaagcgc atgaagaagt acacgaagac
     4201 cagatcgaga atgacccgga aggtgcgcga gatgccgtat ttggactggc cgaagacccg
     4261 cgcgtggtgg gtgacgacct cctgggcgat cttgcgcggc gtggtgacgg tggccagcca
     4321 ggcggggatg aagcggtgca tctcgccgta caggcgcacg ttcttgatgg cggtggcgcg
     4381 gaaggccttg agggagcagc cgtagtcctg cagatgcacg ccggtgatgc gcgcgatcag
     4441 gcggttggcg atcttggacg ggatcttgcg caggatcatc ccgtccttgc ggttcttgcg
     4501 ccagccggcg accaggtcca ggtcttcccg cagcaggcgg ccgaccatgc gcgggatgtc
     4561 gatggggtcg ttctgcaggt cgccgtccat ggtgacgatc acgtcgccgc gggcggcgtc
     4621 caggccggcc tgcatggcgg cggtctgctt gaagttgcgc atcagggcga cgatgcgcac
     4681 atgagggccg tagcgtttgg cggcggcttc gaggcgcggc acggtgttgt cggagctgcc
     4741 gtcgtccacg atcaccgcct cccacggata ggggtagggg ccgagggcca ggtggatgcg
     4801 ctccagcagc gggtccacgt tgtcctcctc gttgtagagc gggaccacca ccgacaggcg
     4861 atggggcggc aggtcggccg ggatgtcggg cgcagcggcc gggagcgggg aggacagggc
     4921 ggtgtcggtc atggcggaaa tctcggaaaa ggccgcggca ggcgggcgct cagggcgcgg
     4981 gccgtgtgct gtcggcggcg gacgacgcgg attgtacgtc ggcagagggg tcggcgtccg
     5041 gcgcggaggc ctcggccggg gcggcgacgg gcaccagcca ggcgagggcg ccgccgctga
     5101 cggacaccgc gatcacgaac aggtgcaggg ccagggccac ctgcaggccg gtgccgaggg
     5161 cgacgccgct gggtagcagc gccgcggcgg cgccggcctc gtaggtgccg aagccggcca
     5221 cgccctgcac cgggaggatc gccgcgagct cggcgcccag cgcgccggcg gcacccacgt
     5281 cgagggcggc ggggatcagc gcggccagca gccaggcttg ggcggccagc ttgacggacc
     5341 agttggtgag cgtccacagc cagccgaagc gggcgtggcg ggtgctctcg gcgaaggcgt
     5401 cgcgcagctt ggcgagcttg gcagtgaggc cgccgtggtc ggcccaggcg tggggcgccc
     5461 gcgcccagcg gggcagccac caggccagcg cgaacacgct cacggcgcca gccagcttga
     5521 ggagggtcgg caggccaggc cacaccagcg cggccacgcc catcacggcg aaggcgtcct
     5581 gcaggcggaa ccagaacagg gacgccaggg cccgcggcag gggcacgccg aaggtctggc
     5641 gcagcagcag cgggaaggcc gcctcgccgc cgcggaaggg cagcacgttg atcatggcgc
     5701 tgtggatcag cacgatgcgc aggcaggcgc cgaagcgtcc gctggcgtgg cggcggaatt
     5761 cgtcccacac gcgcagggca cgcaggctgt aggacaggcc gaagcccagc gcggacacgg
     5821 ccagcaccga cagcggcacc cgcgcgaaga cctcacccac gccgcgccag cggccgtcgg
     5881 cggcgagcca gccgatgagg gcgagggaca gggcgacggc gaagaggcgt ttgagcgtca
     5941 tgccgcgctc cgctcacgca tcagggcggc agcggccccg ccgatgcccc aggcgcccag
     6001 gccgatctga tataggcgcg cctctggata gcccacattg accagcagca gggccaggct
     6061 cgcgatgagg gcgatgccgg ccatcaggcg gatcgccgcg tccttctgcc agaagcgcag
     6121 gcgggcggtg agggccgcgc cgagggcacc gcacagccag ccgccgcagg cgccgacggc
     6181 cacatccgcc ggccagtggg cccccacggc aatccgcgag aaggctacca gaccggcggc
     6241 caggagaagc agtccccagg tgagggcccg ccgtggggcg tccggccagg ccagcagcac
     6301 cgctgcggcg acgacgaagg cggtggtggc gtggccggac gggaaggcgt ccacgtaaa
//
LOCUS       DAOQPA010000159         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7097_length_6355_cov_19.861905, whole genome shotgun sequence.
ACCESSION   DAOQPA010000159 DAOQPA010000000
VERSION     DAOQPA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_6355_cov_19.861905"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /gene="hisH"
                     /locus_tag="PKX01_15590"
     CDS             <1..123
                     /gene="hisH"
                     /locus_tag="PKX01_15590"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989099.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMZ77607.1"
                     /translation="YGIPFTGAVAQANIFAVQFHPEKSAQAGLQLLSNFMSWKP"
     gene            187..933
                     /gene="hisA"
                     /locus_tag="PKX01_15595"
     CDS             187..933
                     /gene="hisA"
                     /locus_tag="PKX01_15595"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMZ77608.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGEMDAATVFSEDPRAMARHWLDQGA
                     RRLHLVDLNGAFAGKPKNEAAIRSILAEVGDEIPVQLGGGIRDLDTIERYLDAGLSYV
                     IIGTAAVKNPGFLHDACSAFAGHILVGLDAKDGKVAVDGWSKLTGHDVVDLARKFEDY
                     GVEGVIYTDIGRDGMLSGVNVEATVKLAQALRIPVIASGGIAAMADVEALCAVESEGI
                     TGAITGRAIYEGTLDFSAAQARADALSGAA"
     gene            938..1696
                     /gene="hisF"
                     /locus_tag="PKX01_15600"
     CDS             938..1696
                     /gene="hisF"
                     /locus_tag="PKX01_15600"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HMZ77609.1"
                     /translation="MLAKRIIPCLDVNAGRVVKGVNFVELRDAGDPVEVARRYDEQGA
                     DEITFLDITASSDDRNIILHVVEQVAEQVFIPLTVGGGVRTVDDVRRLLNAGADKVSI
                     NTAAVNNPQVVAEASGKVGSQCIVVAIDAKQVAPGRWEVFTHGGRNRTGLDAVEWARK
                     VESLGAGEILLTSMDRDGTKNGFDLGLTRAVSDAVNIPVIASGGVGNLDHLADGVSLG
                     RADAVLAASIFHFGDHTVREAKELMRARGIEVRL"
     gene            1693..2082
                     /gene="hisI"
                     /locus_tag="PKX01_15605"
     CDS             1693..2082
                     /gene="hisI"
                     /locus_tag="PKX01_15605"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HMZ77610.1"
                     /translation="MSEAWLDAVAWDAQGLVPAIAQDAETGEVLMFAWMNRDALARTV
                     ATGEAVYFSRSRGRLWHKGEESGHIQKVRELRIDCDNDVVLLKIEQIGGMACHTGRRS
                     CFFQKYFADGRWQAVDPVLKDPKDIYK"
     gene            2079..2405
                     /locus_tag="PKX01_15610"
     CDS             2079..2405
                     /locus_tag="PKX01_15610"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HMZ77611.1"
                     /translation="MIDIEVLHRVAQTLAERKEADPESSYVSGLFHKGTDAICKKVAE
                     EAAETIMAAKDKDLLHVVWEVTDVWFHTMILLAHFGLSVDDVLAEFRRREGVSGIDEK
                     KARTAK"
     gene            2418..2768
                     /locus_tag="PKX01_15615"
     CDS             2418..2768
                     /locus_tag="PKX01_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HMZ77612.1"
                     /translation="MQECLFCRIVRGEIPAAKVYEDDEVLAFKDIHPQRPVHVLVIPK
                     HHITSLAHASEADVPVLGRMLAVAHRIAADQGSPNGFRSIINTGPVGGQEVMHVHMHI
                     VGGQEPVGPMLRRI"
     gene            2779..3003
                     /gene="tatA"
                     /locus_tag="PKX01_15620"
     CDS             2779..3003
                     /gene="tatA"
                     /locus_tag="PKX01_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HMZ77613.1"
                     /translation="MGSFSIWHWLIVLVIVMLVFGTKKLRNIGEDLGGAVKGFKQGMR
                     EGEGAAGDPQKIAGAEGRTIDAEAREKNKS"
     gene            3123..3710
                     /gene="tatB"
                     /locus_tag="PKX01_15625"
     CDS             3123..3710
                     /gene="tatB"
                     /locus_tag="PKX01_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HMZ77614.1"
                     /translation="MFDIGFSELIVIGVVALIVLGPERLPKVARTTGHLLGRLQRYVA
                     DVKSDIHREMQIDELKRLQDEARQAALQMETSVRDEMAHFKGEVDSAVQGAASSVAEL
                     ERELQAAAPSAEPDAQSEAPSAPSASPSADVTDADAAAQRLDRQLAEAEQHAGAPVFA
                     EAQPDGSDAAMPDDRQLGFDFAAAEAAPAAKAEQS"
     gene            3707..4453
                     /gene="tatC"
                     /locus_tag="PKX01_15630"
     CDS             3707..4453
                     /gene="tatC"
                     /locus_tag="PKX01_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMZ77615.1"
                     /translation="MNAPQEESFISHLVELRDRLLRAVVAVVLVFVCLMPWAGDIYDL
                     LALPMMANLPEGTRMIATGVVTPFFVPVKVTLLVAFVIALPFVLYQAWAFIAPGLYAH
                     EKRLGLPLVIGSTVLFLAGMAFCYFFVFGTVFKFIAQFAPKSITPAPDIEQYLAFVMS
                     MFVAFGITFEVPVIVILLVKIGLVDVAKLKEVRPYVIVGAFIVAAVVTPPDVVSQLML
                     AIPMCLLYEAGVFLARFVVKREVGVESGSA"
     gene            complement(4464..5045)
                     /locus_tag="PKX01_15635"
     CDS             complement(4464..5045)
                     /locus_tag="PKX01_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ77616.1"
                     /translation="MPDDTSYYVYALKDPRRSPALPFYIGKGTGTRAHDHLIKPDGTR
                     KGARIREIVDSGGHVLVTRLVDALTEQQALSLEAELIAAFGTLDTGGLLTNTVVPSGL
                     SRKTKPAIVVPSGVREKAQLGLALLKDAILEMAKANPRGITNSDAASLLGLRSDYGGG
                     SKDYLSYSVIGLLMREGKVQRDGASRRHIARVE"
     gene            complement(5091..6182)
                     /gene="ychF"
                     /locus_tag="PKX01_15640"
     CDS             complement(5091..6182)
                     /gene="ychF"
                     /locus_tag="PKX01_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMZ77617.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDPRLAALSEIVKPQKVQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDENVVHVNGKVDPLADIETIVTELALADMSAVEKAIHRENKKARAGDKDAQK
                     LVALLERLMPHLNEGQPVRTLSLSDDEKQLLKPLCLMTLKPAMYVGNVLEDGFENNPY
                     LERLREHAAEEGAPVVALCAKIEAELADLDDADKLAFLADLGLDEPGLNRLIRAGYKL
                     LGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHTDFERGFIRAQTIAYEDFITYKGEA
                     GAKEAGKMRSEGKDYVVKDGDVLNFLFNV"
BASE COUNT         1060 a         2023 c         2109 g         1163 t
ORIGIN      
        1 tacggcatcc cctttaccgg ggcggtagcg caggctaata tctttgccgt ccagttccat
       61 ccggaaaaaa gcgcgcaagc gggcctgcaa ctcctgtcga acttcatgtc ctggaagccc
      121 tgatggcccg cccgcatcgc ctgggcgtct cccccgtccc gatccccttc ctcctccgca
      181 ctccctatgc tgctgattcc cgctattgac ctgaaggatg gtcactgcgt gcgcctgaaa
      241 caaggcgaga tggacgccgc cacggtgttt tccgaagatc cccgcgccat ggcccgacac
      301 tggctcgacc agggggcgcg ccgcttgcat ctcgtggacc tgaacggcgc cttcgccggc
      361 aagcccaaga acgaggcggc gatccgctcc atcctggccg aggtgggcga cgagatcccc
      421 gtccagctgg ggggcggcat ccgcgacctg gacaccatcg agcgctacct cgacgccggt
      481 ctgtcctatg tgatcatcgg cactgcggca gtcaagaatc cgggtttcct gcacgatgcg
      541 tgcagcgcct ttgccggcca catcctcgtc gggctcgacg ccaaggacgg caaggtggcg
      601 gtggatggct ggtccaagct caccggtcat gacgtggtcg accttgcccg caagttcgag
      661 gattacggcg tcgaaggtgt gatctacacc gacatcggcc gcgacggcat gctcagcggc
      721 gtcaacgtgg aggccaccgt caagctggcc caggcgctgc gcatcccggt catcgccagc
      781 ggcggcatcg ccgccatggc ggacgtggaa gccctgtgcg ccgtggaatc cgaaggcatc
      841 accggtgcca tcaccggccg cgcgatctac gagggcacgc tggatttctc cgccgcccag
      901 gcccgcgccg acgcactctc cggagcggcc tgatcccatg ctcgccaagc gcattatccc
      961 ctgtctcgac gtaaacgccg ggcgtgtcgt caagggcgtc aatttcgtcg aactgcggga
     1021 tgccggcgat ccggtcgaag tcgcgcgccg ctacgacgaa cagggcgccg acgagattac
     1081 cttcctcgac atcacggcca gttccgacga ccgtaacatc atcctccacg tggtggagca
     1141 ggtggccgag caggtcttca ttcccctcac cgtgggcggc ggtgtgcgca ccgtagacga
     1201 cgtgcgccgg ctcctcaatg cgggggccga caaggtcagc atcaacaccg ccgcggtcaa
     1261 taatccgcag gtggtggctg aggcctccgg caaggtgggc agccagtgca tcgtggtggc
     1321 catcgacgcc aagcaggtgg cgccgggccg gtgggaagtc ttcacccacg gtggccgcaa
     1381 ccggaccgga ctggacgccg tggaatgggc gcgcaaggtg gaatccctgg gcgccggcga
     1441 gatcctcctc accagcatgg accgggacgg caccaagaac ggcttcgacc tgggcctgac
     1501 ccgtgccgtg tcggacgcgg tcaacatccc cgtcatcgcc agcggtggcg tgggcaatct
     1561 ggaccatctg gccgacggcg tcagcctcgg ccgcgccgat gccgtgctgg ccgccagcat
     1621 cttccacttc ggcgaccaca ccgtgcgcga ggccaaggag ctcatgcgcg cccgtggcat
     1681 cgaggtacgc ctgtgagcga agcgtggctt gatgccgtcg cctgggatgc ccagggcctc
     1741 gtgcccgcca tcgcccagga cgccgagacc ggcgaggtgc tcatgttcgc gtggatgaat
     1801 cgcgacgccc tggcccgcac ggtggccacc ggcgaggcgg tatacttctc gcgctcccgc
     1861 ggcaggctgt ggcacaaggg cgaagagtcc ggccacatcc agaaggtccg cgagctgcgt
     1921 attgactgtg acaacgacgt ggtactcctg aagatcgagc agattggcgg catggcctgt
     1981 cataccgggc gccgcagctg ctttttccag aagtacttcg cggacggccg ctggcaggcc
     2041 gtcgacccgg ttctcaaaga cccgaaggac atctacaaat gatcgacatc gaagtgctgc
     2101 accgcgtcgc ccagaccctg gccgaacgca aggaagcgga tcccgagtcc tcctatgtgt
     2161 cgggcctgtt ccacaagggc accgacgcca tctgcaagaa ggtcgccgaa gaggccgccg
     2221 agaccatcat ggcggccaag gacaaggacc tcctccacgt ggtctgggaa gtgaccgacg
     2281 tgtggttcca caccatgatc ctgctcgccc acttcggact gtccgtcgac gacgtgctgg
     2341 ccgagttccg ccgccgcgaa ggtgtctcgg gcatcgacga aaagaaggcg cgtaccgcca
     2401 agtaaggaac tgtccccatg caggagtgtc tgttctgccg catcgtgcgg ggcgagatcc
     2461 cggctgccaa ggtgtacgaa gacgacgagg tgctggcctt caaggacatc cacccgcaac
     2521 gtcccgtgca tgtgctggtg atccccaagc atcacatcac ctcgctggcc cacgcctccg
     2581 aggcggacgt gcccgtgctg gggcggatgc tggcggtggc tcatcggatt gctgccgatc
     2641 aaggcagtcc gaacgggttc cggtccatca tcaacaccgg ccctgtcggc ggacaggaag
     2701 tgatgcacgt gcacatgcac atcgtgggcg gacaggagcc ggtcggaccg atgctccggc
     2761 gcatttaagg agaagggaat gggttctttc agtatttggc actggctgat cgtgctggtg
     2821 atcgtgatgc tcgtgttcgg caccaagaag ctgcgcaaca tcggcgagga tctgggcggt
     2881 gccgtcaagg gcttcaaaca gggcatgcgc gaaggtgaag gtgctgccgg cgacccccag
     2941 aagattgcgg gcgccgaggg ccgcacgatc gacgccgagg cccgtgagaa gaacaagtcc
     3001 tgatctccgt cgcggagcag gcgcagcaag gggcgcgtca ggcggcccct tttgtttttg
     3061 taaaccgggg ctgatgcagg cgtcacgcgc gtgccgcggt cccgtcttgt cgggctgtcc
     3121 ggatgtttga tatcggtttt tctgagctca tcgtcattgg cgttgtcgcg ctgatcgtcc
     3181 tcgggccgga gcgtctgccc aaggtggcgc gtaccaccgg gcacctgctc ggccgtctgc
     3241 agcgttacgt cgccgatgtg aagtcagaca tccaccggga aatgcagatc gacgagctca
     3301 agcgcctgca ggacgaagcg cgccaggctg ccttgcagat ggaaacctcc gtccgcgacg
     3361 agatggcgca tttcaagggc gaagtggatt ccgcggtgca gggcgctgcg tcgtcggtgg
     3421 ccgagctgga gcgggaactc caggcagccg cgccgtctgc cgagccggac gcgcagtccg
     3481 aagcgccgtc tgccccgtct gcctcccctt cggcagatgt gaccgatgcg gatgccgcgg
     3541 cgcagcgcct cgaccgccag cttgccgagg ccgagcagca tgccggtgcg ccggtctttg
     3601 ccgaggcgca gccagacggc agcgacgccg ccatgcccga tgaccgtcag ctcggcttcg
     3661 actttgccgc tgcggaagcc gcccccgctg ccaaggccga acaatcatga atgcccctca
     3721 ggaagaaagc ttcatctccc atctggtcga actgcgcgac cgcctgctgc gggccgtcgt
     3781 ggcagtggtg ctggtctttg tgtgcctgat gccctgggcc ggcgacatct acgacctgct
     3841 cgccctgccg atgatggcca acctgccgga aggcacccgc atgatcgcca ccggcgtggt
     3901 cacgcccttc ttcgtgccgg tcaaggtgac cctgctggtg gccttcgtca tcgcgttgcc
     3961 ctttgtgctc taccaggcct gggctttcat cgcgccgggg ctctacgccc acgaaaagcg
     4021 cctgggcctg cccctggtga ttggcagcac ggtgctgttc ctggcgggca tggccttctg
     4081 ctacttcttc gtgttcggca cggtcttcaa gttcatcgcc cagttcgcgc ccaagagcat
     4141 caccccggcg ccggacatcg agcagtacct ggccttcgtg atgtccatgt tcgtcgcctt
     4201 cggcatcacc ttcgaggtgc cggtgatcgt cattctgctg gtcaagatcg gcctggtgga
     4261 tgtggccaag ctgaaggaag tccgccccta cgtgatcgtc ggcgccttca ttgtcgcagc
     4321 cgtggtcacc ccgccggacg tggtgtcgca gctgatgctg gcgatcccca tgtgcctgct
     4381 ctacgaagcc ggtgtgttcc tggcgcggtt cgtggtcaag cgcgaggtgg gtgtggagag
     4441 cgggtcggcc tgacgcgggg ccctcactcg acccgggcga tgtgtcgtcg cgaagcccca
     4501 tcgcgctgga ctttgccttc ccgcatgagc aggccaatga cactgtaaga cagatagtcc
     4561 tttgatccgc cgccatagtc gctgcgcaat cccagcaggc tcgcggcgtc ggaattggtg
     4621 atgccccggg gattcgcctt ggccatttcg aggatggcgt ccttgagcag ggcgagcccg
     4681 agttgcgcct tttcccgcac gccggatggg acaacaatgg cgggcttcgt ctttcttgaa
     4741 aggcccgagg gcacgactgt attggtcagt aacccacctg tatccagtgt gccgaatgcg
     4801 gcgatcaatt cggcttcaag cgagagcgct tgctgctcgg tcagcgcatc gaccagccgc
     4861 gtaaccagca cgtgaccgcc ggaatcgacg atctcgcgga tgcgggcgcc tttgcgggtg
     4921 ccatccggct tgatcaggtg gtcgtgggcg cgcgtgccgg tgcccttgcc gatgtagaag
     4981 ggaagcgccg gggacctgcg tgggtctttc agcgcataca cgtaataaga cgtgtcgtcc
     5041 ggcatggtga aaggccccgc tgttgctgag atggtacgcc ctgtgccgaa tcagacgttg
     5101 aacaaaaagt tcagcacgtc cccgtccttc accacgtagt ccttgccttc cgagcgcatc
     5161 ttgccggctt ccttggcgcc agcttcgccc ttgtaggtga tgaagtcttc gtaggcgatg
     5221 gtctgggcgc ggatgaagcc gcgttcgaag tcggtgtgga tgacgccggc ggcctggggg
     5281 gcggtgtcgc ccacgtggat ggtccaggcg cggacttcct tgacgccggc ggtgaagtag
     5341 gtttgcaggc cgaggagctt gtagccggcg cggatgaggc ggttcaggcc gggttcgtcg
     5401 aggcccaggt cggccaggaa ggccagcttg tcagcgtcgt ccagatcagc cagttcggcc
     5461 tcgatcttgg cgcacagggc gacgaccggc gcgccttcct cggcggcgtg ttcgcgcagg
     5521 cgctccaggt aggggttatt ctcaaagccg tcttcgagca cgttgcccac gtacatggcg
     5581 ggcttgaggg tcatcaggca caggggcttg agaagttgct tctcgtcatc gctcagggac
     5641 agggtgcgta ccggctggcc ttcgttgagg tggggcatca gccgttcaag cagggcgacg
     5701 agcttctggg cgtccttgtc gccggcacgg gccttcttgt tttcccgatg gatggccttt
     5761 tcgacggcgg acatatcggc gagggcgagt tcggtgacga tggtctcgat gtcggcgagg
     5821 ggatcgacct tgccgttcac gtgcaccacg ttttcgtcgt cgaagcagcg caccacattg
     5881 acgatggcgt cggtctcgcg gatgttggca aggaactggt tgcccaggcc ttcgcccttg
     5941 gaggcgccgg cgacgaggcc ggcgatgtcc acgaattcga cgatggccgg ctggaccttc
     6001 tggggcttga cgatctcgga gagggcggcg aggcgcgggt cgggcacttc gacgatgccg
     6061 acgttgggct cgatggtgca gaaggggtag ttctcggccg cgatgccggc cttggtgagg
     6121 gcgttgaaga gagtggactt gccgacgttg ggcagaccca cgatgccgca cttgagagac
     6181 atggaaatcc tttgtggtca ggggcttccg gaggggaagc gggacagccc gctattgtaa
     6241 ggcgggcgtc gcctcgggcc caaggaattc ggcggcgcgg gcagagtcga ctgcgcaacc
     6301 cgcaacccgc aacccgcaac ccgcaacccg caacccgcaa cccgcaaccc gcaac
//
LOCUS       DAOQPA010000160         6184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7432_length_6184_cov_20.572035, whole genome shotgun sequence.
ACCESSION   DAOQPA010000160 DAOQPA010000000
VERSION     DAOQPA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7432_length_6184_cov_20.572035"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PKX01_15645"
     CDS             <1..1752
                     /locus_tag="PKX01_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HMZ77618.1"
                     /translation="QTGKISRFGLLELSRQRLRPALAETSYIPCPRCNGTGHIRSTES
                     SALHILRILEEEAMKDNTGALHCQVPVDVATFLLNEKRDDIAKIEVRHKVNLILIPNR
                     HLETPQHEIVRLRHDQLNQEEHVLPSYKMATQPSDVTYTPPSASNDRPARQEAAVKGI
                     TPDQPAPTVEAKPAPAPAPVPESQPGLLARIFAFFRGEKQPEPAPVVAQPEPARRESR
                     SRNDRNRRDGRNRRDGQREERGERAERSERSERNEPRDTRAPEAAAETREKPQRQRGE
                     RQERGERGERPERAERGERSERGEGRGRNRNSAQAPRAVETEAAVSDADNKLEAVASV
                     AVVDAAMPAPTSEGEGNGERNRRRRGRRGGRRNEESGTSESAAQGELEIGTAAAEVAE
                     AVPAAEADTPAVTDTPAIEPAVVEAVVPQQVETVSAAAASVEPVFEPAAEVASDAAPV
                     TAEAPVVDAVAPVAAVEETVPQETPTEPETAPVPEPGVADAPAAAPSFFATPVEVPLE
                     APAAAPVVETLPVVEEAVATLDETLKDSGLILIETRRDAAAPVAPVEPAQPLGRRRRA
                     VAPVAEEPLVQVETGAK"
     gene            complement(1860..2354)
                     /locus_tag="PKX01_15650"
     CDS             complement(1860..2354)
                     /locus_tag="PKX01_15650"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMZ77619.1"
                     /translation="MMKILFVCTGNICRSPTAEGVARRWLAMTGLEGIVGVDSAGIQG
                     YHAGEAPDPRAQTAAMRRGYDLSRLRARKVLLKDFAEFDLILAMDRDHFHYLRDMAPE
                     GCKHKVRMFMSFAQTHRRDDVPDPYYGGITGFDAVLDMCEDAVQGLIDDIRPDVERRH
                     RSVV"
     gene            complement(2435..4513)
                     /gene="uvrB"
                     /locus_tag="PKX01_15655"
     CDS             complement(2435..4513)
                     /gene="uvrB"
                     /locus_tag="PKX01_15655"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMZ77620.1"
                     /translation="MADQTDSGRVVCFEGSPFRLHQPFPPAGDQPEAIRLLAEGIGDG
                     LMFQTLLGVTGSGKTYTMANVIARCGRPALVLAHNKTLAAQLYAEFREFFPENAVEYF
                     VSYYDYYQPEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLMERRDVVIVASVSCIYG
                     IGDPVDYHAMVLHLREGERMAHRDLVQRLVAMQYTRSDVDFRRGTFRVRGDVIDIFPA
                     ENAEYALRVEMFDDEIEHLTLFDPLTGHLKQKIVRFTVYPSSHYVTPRATVLRAIEAI
                     KDELRTRIQTFQTGGKLVEAQRIEQRTRFDLEMLNELGFCKGIENYSRHLSGRAAGEP
                     PPTLIDYLPADGLMFIDESHVTIGQVGAMYKGDRSRKENLVEYGFRLPSALDNRPLKF
                     EEFERLLPQTVFISATPAAYEAAHQGQVVEQVVRPTGLVDPVIHVRPASTQVDDLLGE
                     IKLRVAVEERVLVTVLTKRLAEDLTEYLAENGIRVRYLHSDIDTVERVEIIRDLRLGH
                     FDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLHGTAILYA
                     DRITDSMRAAMDETERRRTKQLAFNAENGITPRSVTKRIKDIIDGVYDAKDEDAAVVA
                     AEPAAEYAAMDEKALARTIRQLEKDMLEHARNLDFEKAAAVRDQLFRLRRQAFGAETH
                     DTSLPPDANR"
     gene            4632..5840
                     /locus_tag="PKX01_15660"
     CDS             4632..5840
                     /locus_tag="PKX01_15660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HMZ77621.1"
                     /translation="MSSSIFAAVDMAPRDPILGLNEAFNADTRNTKVNLGVGVYFDDN
                     GKIPLLAAVKTAEKARLEAAPPRGYQPIEGPAAYNNAVQNLLFGAASPLLSDGRVVTA
                     QALGGTGALKIGADLLKRVVPGATVYISDPSWENHRALFEAAGFPVENYPYYDPATRG
                     ANAEGMIATLNSLPAGSIIVLHACCHNPTGADLTEAQWAEVVKACATRGLVPFLDMAY
                     QGFADGIEPDAVAVNLFAASGLSFFVSSSFSKSFSLYGERVGALSIVTQNKDESARVL
                     SQLKRVIRTNYSNPPIHGGAIVAAVLSTPELRAQWEAELAGMRERIRAMRTGLVEQLA
                     ARKVDQDFSFVIKQRGMFSYTGLSAAQVERMKTEFGIYAVGTGRICLAALNSKNLDYV
                     ADAIAAVLKA"
     gene            5937..6012
                     /locus_tag="PKX01_15665"
     tRNA            5937..6012
                     /locus_tag="PKX01_15665"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5970..5972,aa:Asn,seq:gtt)
     gene            6062..6137
                     /locus_tag="PKX01_15670"
     tRNA            6062..6137
                     /locus_tag="PKX01_15670"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6095..6097,aa:Asn,seq:gtt)
BASE COUNT         1089 a         2206 c         1905 g          984 t
ORIGIN      
        1 cagaccggca agatcagccg cttcggcctg ctcgaactgt cccgccagcg cctgcgcccc
       61 gccctcgccg aaaccagcta catcccctgc ccgcgctgca acggcaccgg ccacatccgc
      121 agcaccgaat cctcggccct gcacatcctg cgcatcctcg aagaagaggc catgaaggac
      181 aacaccggcg ccctgcactg ccaggtcccc gtcgatgtgg cgaccttcct cctcaacgaa
      241 aagcgcgacg acatcgccaa gatcgaggtg cgccacaagg tcaacctcat cctcatcccc
      301 aaccgtcacc tggaaacccc gcagcacgaa atcgtgcgcc tgcgtcacga ccagctcaac
      361 caggaagagc acgtcctgcc cagctacaag atggcgaccc agccgtctga cgtgacctac
      421 accccgccgt cggccagcaa tgatcgtccg gcccgccagg aagccgccgt caagggcatc
      481 actccggacc agccggcccc caccgtggaa gccaagcccg caccggcccc tgcccccgtg
      541 cccgaaagcc agcccggcct gctcgcccgc atcttcgcct tcttccgcgg cgagaagcag
      601 ccggagcccg cgccggtcgt cgcccagccg gaacctgccc gtcgcgaatc ccgctcccgc
      661 aacgaccgca accgccggga tggccgcaac cgtcgcgacg gccagcggga agagcgcggc
      721 gaacgcgctg aacgttccga gcggagcgag cgtaacgagc cccgcgacac ccgcgcaccc
      781 gaagccgccg ccgaaacccg cgaaaagccg cagcgccagc gcggcgaacg ccaggagcgc
      841 ggcgaacggg gcgagcgtcc ggaacgcgcc gagcggggtg aacgcagcga gcgcggcgaa
      901 ggccgcggcc gcaaccgcaa tagcgcccag gcaccgcgcg ccgtcgagac ggaagccgcg
      961 gtcagcgatg ctgacaacaa gcttgaagcc gttgccagtg tcgccgtcgt ggacgccgcg
     1021 atgcctgcgc cgaccagtga aggcgaaggc aatggcgagc gcaatcgccg tcgccgcggc
     1081 cgtcgcggcg gtcgtcgcaa cgaggagtcc ggcaccagcg agagcgctgc ccagggtgag
     1141 ctcgagattg gcaccgccgc agcggaggtc gccgaagccg tgcccgcggc tgaagccgac
     1201 accccggccg tgaccgacac gcctgccatc gagccggccg tggtcgaggc cgtcgtgccg
     1261 cagcaagtgg aaaccgtgag tgccgcggct gcatcggtcg aacccgtgtt cgagccggcc
     1321 gcagaggtgg cgtccgacgc ggccccggtg acggccgaag cgcccgtggt ggacgcggtc
     1381 gcccccgtcg cagccgtgga ggaaaccgtg ccccaggaaa cgccgaccga acccgagacg
     1441 gcgcctgtgc ccgagcccgg cgtggctgac gcgcccgcgg cggccccatc cttcttcgcg
     1501 acgcccgttg aagtgcccct ggaggcgcct gcggcggcac ccgtcgtcga gaccctgccc
     1561 gtggtcgaag aagcggtcgc cacgctggac gagaccctca aggacagcgg cctgatcctc
     1621 atcgagaccc gtcgtgacgc tgccgcaccc gtggcgccgg tcgaaccggc ccagcctttg
     1681 ggacgccgtc gtcgcgcggt ggcaccggtg gcggaggagc cgctcgttca ggtggaaacc
     1741 ggggccaagt aaggaccggc gatcccgcca acaaaaaggc caacccgcag gggttggcct
     1801 ttttcatggg cgcgtggcgg actgcgcgcc gcttccggcg ctcgacgcac gctgcgcgtc
     1861 tacaccaccg agcggtgacg gcgctccacg tccggacgga tatcgtcgat cagtccctgc
     1921 accgcgtcct cgcacatgtc cagcaccgcg tcgaatccgg tgatcccgcc atagtaagga
     1981 tcgggcacgt catcgcggcg atgggtctgg gcgaagctca tgaacatgcg caccttgtgc
     2041 ttgcagcctt ccggcgccat gtcccgcaga tagtggaaat gatcccgatc catggccagg
     2101 atcaggtcga attcggcgaa gtccttgagc agcaccttgc gtgcgcgcag ccgcgacaga
     2161 tcgtagccac gccgcattgc tgcggtctgg gctcgtggat ccggcgcttc accggcgtgg
     2221 taaccctgga tgcccgccga atcgactcca acgatgccct ccagacccgt catggccaac
     2281 cagcggcgcg caaccccctc cgccgtcggc gaccggcaga tattgccggt gcaaacaaat
     2341 aatattttca ttatgggcgt ccctcctcct gctggtaatt taagcaggcg gcgggccgtc
     2401 cgtacaaggg tcagcaccaa aatcttccaa tcgatcagcg gttcgcgtcc ggagggaggc
     2461 tcgtatcgtg ggtttcggcg ccgaaggcct ggcggcgcaa gcggaacaac tggtcccgca
     2521 cggcggcggc cttctcgaaa tccagattgc gggcgtgttc gagcatgtcc ttttcgagtt
     2581 gccggatcgt cctggccagc gccttctcgt ccatcgcggc atactccgca gcgggctcgg
     2641 cagccacgac agcagcatcc tcgtccttcg catcatatac gccgtcgatg atgtctttta
     2701 tgcgctttgt cacgctgcgc ggagtaatgc cgttctcggc attgaaggcc agctgtttgg
     2761 tgcgacggcg ctcggtctcg tccatggcgg cgcgcatgga atcggtgatc cggtcggcgt
     2821 agaggatggc cgtgccgtgc agatgacgcg cggcgcggcc aatggtctgg atcagggacc
     2881 gctccgagcg caggaagccc tccttgtccg catcgaggat tgccaccagg gacacttcgg
     2941 gaatgtccag cccctcccgc agcaggttga tgcccaccag cacgtcgaag tgcccgaggc
     3001 gcagatcgcg gatgatctcg acgcgctcca ccgtgtcgat gtccgagtgc aggtagcgca
     3061 cgcggatgcc gttctctgcc aggtattccg tgagatcctc cgccagccgc ttggtgagca
     3121 ccgtcaccag tacgcgctcc tcgacggcga cccgcagctt gatctcgccg agcagatcgt
     3181 ccacctgggt agatgccggc cgcacgtgga tcaccggatc caccagcccg gtcggccgca
     3241 ccacctgctc cacgacctgg ccctggtgtg ccgcttcgta agccgccggc gtcgccgaga
     3301 tgaacacggt ctggggtagc agacgctcga actcctcgaa cttcagcggc cggttgtcca
     3361 gcgccgacgg caggcggaag ccgtactcca ccaggttttc cttgcgcgaa cgatcgccct
     3421 tgtacatggc gcccacctgg ccgatggtga cgtgggattc gtcgatgaac atcagcccgt
     3481 ccgccgggag gtagtcgatc agcgtcggcg gcggctcgcc cgcggcacgg ccggagaggt
     3541 gtcgcgagta gttctcgatg cccttgcaga agcccagctc gttgagcatc tccaggtcga
     3601 agcgggtgcg ctgctcgatg cgctgggcct ccaccagctt gccgccggtc tggaaggtct
     3661 ggatgcgtgt gcgcagctcg tccttgatgg cctcaatggc ccgcaacacc gtagcccgcg
     3721 gcgtcacgta gtgactcgac ggatacaccg tgaagcgcac gatcttctgc ttgaggtgtc
     3781 cggtaagagg gtcgaacagg gtcaggtgct cgatctcgtc gtcgaacatc tcgacacgca
     3841 gggcgtactc ggcgttttcc gccggaaaga tatcgatcac gtcgccccgc acacggaagg
     3901 tgccgcgccg gaaatccacg tcggagcggg tgtactgcat tgccaccagg cgctgcacca
     3961 gatcccggtg ggccatgcgc tcgccttcac gcaggtgtag caccatggcg tggtaatcca
     4021 ccggatcgcc gatcccgtag atgcacgaca cggaagccac gatcaccacg tcgcgccgct
     4081 ccatcaggct cttggtggcg gacaggcgca tctgctcgat gtgctcgttg atggacgagt
     4141 ccttctcgat gaagaggtcg cgggacggca cgtaggcttc cggctggtag tagtcgtaat
     4201 agctcacgaa gtactccact gcgttctccg ggaagaactc gcggaactcc gcatacaact
     4261 gcgcggccag cgtcttgttg tgggccagca ccagcgccgg gcggccgcag cgggcgatca
     4321 cgttggccat ggtgtaagtc ttgcccgacc cggtcacacc cagcagggtc tggaacatca
     4381 ggccatcgcc aatgccctcc gccagcaggc ggatcgcctc gggctggtcg ccggccggcg
     4441 ggaagggctg gtgaagacgg aaggggctgc cctcgaagca caccacgcgc ccgctatcgg
     4501 tttgatcggc catgataaaa tgtcagtttt gagtcagaat gccgtgcccg ccgggaaaca
     4561 ccggcagccc tgcattatcg cccagcgcgc gccccgcagc agcgcgcacc cttcgcccaa
     4621 ctggagaatc catgagctct tcgatcttcg ccgcggtgga catggccccc cgcgatccga
     4681 tccttggcct gaacgaagcc ttcaatgcgg acacccgcaa caccaaggtc aacctcggcg
     4741 tcggcgtcta cttcgacgac aacggcaaga tccccctgct ggccgccgtc aagaccgccg
     4801 aaaaggcccg cctcgaagcg gcgccgccgc gcggctacca gcccatcgaa ggccctgccg
     4861 cctacaacaa cgccgtgcag aacctgctct tcggcgctgc cagcccgctg ctgtccgatg
     4921 gccgtgtggt caccgcccag gccctcggcg gcaccggcgc cctcaagatc ggtgccgacc
     4981 tgctcaagcg cgtcgtgccc ggcgccaccg tttacatctc cgatccgtcc tgggaaaacc
     5041 accgtgccct cttcgaggcc gccggcttcc cggtcgagaa ctacccctat tacgatccgg
     5101 ccacccgcgg cgccaacgcc gaaggcatga tcgccacgct caacagcctg ccggccggtt
     5161 ccatcatcgt cctgcacgcc tgctgccaca acccgaccgg cgccgacctc actgaagccc
     5221 agtgggctga ggtcgtcaag gcctgtgcca cccgcggcct ggtgcctttc ctcgacatgg
     5281 cctaccaggg cttcgccgac ggcatcgaac cggacgccgt ggcagtcaac ctgttcgccg
     5341 cctccggcct gtccttcttt gtttccagct ccttttcgaa gagcttttcg ctgtacggcg
     5401 agcgcgtcgg cgccctgtcc atcgtcaccc agaacaagga cgagtccgcc cgcgtactgt
     5461 cccagctcaa gcgcgtgatc cgcaccaact actccaaccc gccgatccac ggcggcgcca
     5521 tcgtcgcggc ggtgctgtcc acccccgaac tgcgcgccca gtgggaagcc gaactggccg
     5581 gcatgcgcga gcgcatccgt gccatgcgca ccggcctcgt cgaacagctt gccgcccgca
     5641 aggtcgacca ggacttctcc ttcgtgatca agcagcgcgg catgttctcc tacaccggcc
     5701 tctccgccgc ccaggtggag cgcatgaaga ccgaattcgg catctacgcc gtcggcaccg
     5761 gccgcatctg cctggctgcg ctgaacagca agaacctgga ctacgtcgcc gacgccatcg
     5821 ccgccgtcct caaggcctga ccctcaccct gccccggcat ccccggggcg ggtgtgttgc
     5881 gcgcgcatga tcgccttgac gtgcactgtc ttcgcaacta gaatgcgcgc ctctgttcct
     5941 cgatagctca gtcggtagag cgccggactg ttaatccgta ggtccctggt tcgagcccag
     6001 gtcgaggagc cagaacactt ttcggtgatg cattcctgaa atgcgtaacc accccgtcag
     6061 atcctcgata gctcagtcgg tagagcgccg gactgttaat ccgtaggtcc ctggttcgag
     6121 cccaggtcga ggagccaaga caagcaagca gaaaagccaa tccgcgaggg ttggcttttt
     6181 tgtt
//
LOCUS       DAOQPA010000161         6066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7646_length_6066_cov_16.179837, whole genome shotgun sequence.
ACCESSION   DAOQPA010000161 DAOQPA010000000
VERSION     DAOQPA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_6066_cov_16.179837"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKX01_15675"
     CDS             complement(<1..994)
                     /locus_tag="PKX01_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HMZ77622.1"
                     /translation="MSAPLLPYAYARSHGVFVASHGPEHADLILREGADLAAVAEVRR
                     SLGVPIRLESVGRAAFEARLAEAYSGADDNAAEVVADVGQEVDLTQLMTELPAVEDLL
                     ETQDDAPIIRMINALLTQAVRQAASDIHIEPYERHSVVRFRRDGVLVDVAQPHRALHA
                     AMASRIKIMASLDIAEKRLPQDGRIALRLAGRQVDVRVSTLPTTHGERIVLRLLDKGS
                     GQRGLESLGMAADTRGTFETLLREPHGILLVTGPTGSGKSTTLYAALQAMDARTLNIV
                     TVEDPVEYDLPGVGQLQVNSRIDLSFAKALRAILRQDPDVIMIGEIRDLETAQIA"
     gene            complement(991..3150)
                     /gene="gspD"
                     /locus_tag="PKX01_15680"
     CDS             complement(991..3150)
                     /gene="gspD"
                     /locus_tag="PKX01_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HMZ77623.1"
                     /translation="MNPARRILAACLAAALALPLPPALAAKGDDADNRVSLNFVNADI
                     GAVIQAVSKISGRNFVVDPRVKGTLNIVTARPVERRNTYPILLSALRLQGYAAIEGDG
                     VTKIVPEADAKLHAVPVGKGQGSGGGDRLTTQVFTLKHESAAQIVPVLRPLISPNNTV
                     TAYPGNNTLVVTDYAENLGRIAQIIESIDVPQGDMRMLPLKHASALDLAATINRLLGD
                     GSAAGAAGQTGDTSQRVTVIGDARTNSLLIRSDNPSKIQAVRQLVGNLDQPGAAGNIH
                     VVYLKNADATKVAQTLRAVLSGESGTTGTSGSTATSSSFAPTGAGTFNSGTTGSTFGQ
                     NTATTTGGGNFSGSSGTSTGVPNATGGGVVQADPMNNALIITAPDTIYTNLRQVVDQL
                     DRRRAQVYVEALITEVSTDKAAEIGIQWQAGKVGTTSAYGGTAFTSSSSGSNLLTLAT
                     ALGSGTVTSPGNGLNLVLGSGSVTVGGKTIANLNMLARFLESDSRANILSTPNLVTLD
                     NEEAKIVVGRNLPFVTGSYATTGGTTTTNPFTTIERKDVGLTLKVKPQITEGGLVRMQ
                     IYQEASSVIDTTTANTYGPTTNKRSIESTVLVDDGATIALGGLVEDSFSGTIEKVPVL
                     GDIPYLGAFFRYDTRKRTKTNLIVFLRPVILRDAAAIRGISSDRYDYIIGQQRTMDSE
                     EQRFKFDRGLPSLPPAGSPPPATPTTVPQPSDRPAPQ"
     gene            complement(3147..3815)
                     /locus_tag="PKX01_15685"
     CDS             complement(3147..3815)
                     /locus_tag="PKX01_15685"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HMZ77624.1"
                     /translation="MNLPERLDLSRLTRLPLARFAPALAWATALAVCAGVGADLFWRF
                     TAPRAAALPVTPLTDPQEAGRAIAGRHLMGAGERAGPAADAVSPGRYTLQAVVTGAQG
                     RPGWAVIATDGGAQQGYVEGQEISSGVVLARVLPDAVEIALGPVRQTVRLAERAAGGA
                     LGVTPENPPPPAAAFLPQPNPSTPPVLPAAMQGQPPVTSDSPADLQPPSTPAGLPAPT
                     APSQ"
     gene            complement(3926..4783)
                     /locus_tag="PKX01_15690"
     CDS             complement(3926..4783)
                     /locus_tag="PKX01_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HMZ77625.1"
                     /translation="MPSPVRPAAPALLHDLDALSRSSPQRRTLLRALLAGAAAPLAGC
                     GGSIDVGLGSVGTSTSSSSSSSTSSSTCSVIPEETAGPYPADGSNANSNGVVNALTLT
                     GVVRSDIRSSFAGASAVATGIPLTLTLQLVNAANSCANLAGYALYLWHCDRNGLYSLY
                     DSTLLNEDYLRGVQVTDSNGQVSFTTIFPGCYAGRMPHIHFEIYPSLSSAVSAAYKMR
                     TSQLTFPLTTINEAYATSLYSTSAANLSGMSFAADTVFSDGTSLQMASVSGSASSGYT
                     ASLVVAIST"
     gene            complement(4913..5665)
                     /locus_tag="PKX01_15695"
     CDS             complement(4913..5665)
                     /locus_tag="PKX01_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ77626.1"
                     /translation="MLPLLYRDDHLVAIHKPAGLLVHRSELDRHETRFAVQMLRDQLG
                     CHVHPLHRLDRGTSGVLLFALDRDTARLGSALFEHQAVDKTYLAVVRGHPPQSGRIDH
                     PLSRRRDEAEWRGDASSDTAQPAVTDFVRLATCELPFAVDRYPSSRYALLEVRPLTGR
                     RHQIRRHLKHIAHPLIGDATFGKGRHNRFFADHFGCARMLLACTRLRLIHPRSGAPLD
                     LVAPPEGDFLRLLDSLGWRPALPAILRTPPAA"
     gene            5952..>6066
                     /locus_tag="PKX01_15700"
     CDS             5952..>6066
                     /locus_tag="PKX01_15700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ77627.1"
                     /translation="MSLPLIFPLHTRRAARRLASAGFTLLELLVVMTIIGLL"
BASE COUNT          809 a         1715 c         2552 g          990 t
ORIGIN      
        1 cggcgatctg ggcggtttcc aggtcgcgga tctcgccgat catgatcacg tccgggtcct
       61 ggcgcaggat ggcgcgcagg gccttggcga agctgaggtc gatgcggctg ttgacctgga
      121 gctggccgac gccggggagg tcgtattcga ccgggtcttc gacggtgacg atgttgagcg
      181 tgcgggcatc catggcctgc agcgcggcgt agagggtggt gctcttgccg gagccggtgg
      241 ggccggtgac gaggaggatg ccgtggggtt cgcgcagcag ggtttcgaag gtgccgcggg
      301 tgtcggcggc catgcccagg ctctccaggc cgcgctggcc gctgcccttg tcgagcaggc
      361 gcagcacgat gcgttcgccg tgggtggtgg gcagggtgga cacgcgtacg tccacctggc
      421 ggccggccag gcgcagggcg atgcggccgt cctggggcag gcgcttctcg gcgatgtcga
      481 ggctcgccat gatcttgatg cgcgaggcca tggcggcgtg cagggcgcgg tggggctggg
      541 ccacgtccac cagcacgccg tcgcggcgga agcgcacgac ggagtggcgc tcgtagggtt
      601 cgatgtggat gtcgctggcg gcctggcgca cggcctgggt gaggagggcg ttgatcatgc
      661 ggatgatcgg cgcgtcgtcc tgggtttcga gcagatcctc cacggcgggc agctcggtca
      721 tgagctgggt gaggtccact tcctggccca cgtcggcgac gacctcggcg gcgttgtcgt
      781 cggcgccgga ataggcctcg gcgaggcgcg cctcgaaggc ggcgcggccg acgctctcga
      841 ggcggatcgg cacgcccagg ctgcgccgca cttcggcgac ggcggcgagg tcggcgcctt
      901 cgcgcaggat caggtcggcg tgctcgggtc cgtggctggc gacgaagacg ccgtggctgc
      961 gggcgtaggc gtagggcagc agcggcgcgc tcattgcggg gccggacggt cggacggctg
     1021 gggcacggtg gtgggcgtgg ccggcggcgg cgagccggcg ggcggcaggg aaggcaggcc
     1081 gcggtcgaac ttgaagcgct gctcctcgct gtccatggtc cgctgctggc cgatgatgta
     1141 gtcgtagcgg tcggacgaga tgccccggat ggcggcggca tcgcgcagga tgacggggcg
     1201 caggaagacg atcaggttgg tcttggtgcg cttgcgcgtg tcgtagcgga agaaggcgcc
     1261 caaatagggg atgtcgccga ggacggggac cttctcgatg gtgccgctga agctgtcttc
     1321 caccaggccg ccgagggcga tggtggcgcc gtcgtccacc agtacggtgg attcgatgga
     1381 gcgcttgttg gtggtggggc cgtaggtgtt ggcggtggtg gtgtcgatga ccgaggaggc
     1441 ttcctggtag atctgcatgc gcaccagccc gccttcggtg atctgcggtt tgaccttgag
     1501 ggtcaggccc acgtccttgc gttcgatggt ggtgaagggg ttggtggtgg tggtgccgcc
     1561 ggtggtggcg taggagccgg tgacgaaggg caggttgcgg ccgacgacga tcttggcttc
     1621 ctcgttgtcc agggtgacca ggttgggcgt ggacaggatg ttggcgcggc tgtcggattc
     1681 gaggaagcgg gccagcatgt tgaggttggc gatggtcttg ccacccaccg tgacgctgcc
     1741 gctgcccagc accaggttga ggccgttgcc cggcgaggtg acggtccccg agcccagcgc
     1801 agtggccagg gtgagcaggt tggagccgct gctgctggag gtgaaggcgg tgccgccgta
     1861 ggccgaggtg gtgccgacct tgccggcctg ccactggatg ccgatctcgg cagccttgtc
     1921 ggtgctgact tcggtgatga gggcctccac gtacacctgg gcgcggcgcc ggtcgagctg
     1981 gtccaccacc tggcgcaggt tggtgtagat ggtgtccggc gcggtgatga tgagggcgtt
     2041 gttcatcggg tcggcctgca cgacgccgcc gccggtggcg ttgggcacgc cggtgctggt
     2101 gccggagctg ccggagaagt tgccgccgcc ggtggtggtg gccgtgttct gaccgaaggt
     2161 gctgccggtg gtgccgctgt tgaaggtgcc ggcgccggtg ggggcgaagc tggacgaggt
     2221 ggcggtgctg ccgctcgtgc cggtggtgcc ggattcgccg gagagcaccg cgcgcagggt
     2281 ctgggcgacc ttggtggcgt cggcgttctt gaggtaaacg acgtggatgt tgccggccgc
     2341 gccgggctgg tccaggttgc ccacgagctg gcgcacggcc tggatcttgg acgggttgtc
     2401 ggagcggatc agcaggctgt tggtgcgggc gtcgccgatc acggtgacgc gctggctggt
     2461 gtcgccggtc tggcccgccg cgccggcggc cgagccgtcg ccgagcaggc ggttgatggt
     2521 ggcggccagg tcgagggcgg aagcgtgctt gaggggcagc atgcgcatgt cgccctgggg
     2581 cacgtcgatg gattcgatga tctgggcgat gcggccgagg ttctcggcgt agtcggtgac
     2641 caccagggtg ttgttgcccg ggtaggcggt gacggtgttg ttgggggaga tcagcgggcg
     2701 cagcacgggg acgatctggg cggcggattc gtgcttgagg gtgaacacct gggtggtgag
     2761 gcggtcaccg ccaccgctgc cctggccttt gccgaccggc accgcgtgga gcttggcgtc
     2821 ggcttccggg acgatcttgg tcacgccgtc gccctcgatg gccgcgtagc cctgcaggcg
     2881 cagcgcggag agcaggatgg ggtaggtgtt gcggcgttcg acggggcgcg cggtgacgat
     2941 gttgagggtg cccttgacgc gcggatcgac gacgaagttg cgtccggaga tcttggagac
     3001 cgcctggatc accgcgccga tgtctgcatt gacgaaattg agggagaccc ggttgtcggc
     3061 gtcgtcgccc ttggccgcca gggcgggcgg caggggcagg gcgagggcgg cagccagaca
     3121 ggcggcaagg atccggcggg cagggttcat tggctcgggg cggtgggcgc cggcaggccg
     3181 gcgggtgtgg acgggggctg cagatcggcc ggcgagtcgc tggtgacggg cggctgaccc
     3241 tgcatcgcgg ccggcaggac cggcggcgtc gaaggattgg gctggggcag gaaggcggcg
     3301 gcgggcggcg gcgggttttc cggggtgacg ccgagggcgc cgccggcggc ccgttcggcc
     3361 agacgcacgg tctggcggac ggggcctagg gcgatctcga cggcgtccgg cagcacgcgg
     3421 gcgagcacca cgccgctgct gatctcctgg ccttccacgt aaccctgctg ggcgccgccg
     3481 tcggtggcga tcaccgccca gccaggacgg ccctgggcgc cggtgacgac ggcctgaagg
     3541 gtgtagcggc ccgggctcac ggcatcggcg gccggaccgg cgcgctcccc cgcgcccatc
     3601 aggtggcgtc cggcgatggc acggccggcc tcctgggggt cggtcagcgg ggtgaccggc
     3661 agggcggcgg cgcgcggcgc ggtgaaacgc cagaagaggt cggcgccgac ccccgcgcac
     3721 acggcgagcg ccgtcgccca cgcaagcgcc ggggcgaagc gggcgagagg gagccgggtg
     3781 agccgggaga ggtcgagacg ttcggggagg ttcatcgggc gctgcaacag gggccagcaa
     3841 tcttagccgg cgaatgttac cggcaaaagg gccccggtgt cgcgccgcca aacggacggc
     3901 gtgccagtgt tcgcggggga ctgcctcagg tagagatggc cacgaccagg gaggccgtgt
     3961 agccgctgct ggcgctgccg ctgacactgg ccatctgcag gctggtgccg tcgctgaaga
     4021 cggtgtcggc ggcgaagctc atccccgaca ggttggcggc gctggtgctg taaagacttg
     4081 tggcgtaggc ctcgttgatg gtcgtcagcg ggaaggtgag ctgggaggtg cgcatcttgt
     4141 aggcagcgct gaccgccgag gacaggctcg ggtagatctc gaagtggatg tggggcatgc
     4201 ggccggcgta acagccggga aagatggtgg tgaagctgac ctggccattg ctgtcggtga
     4261 cctgcacgcc gcgcaggtag tcctcgttga gcagggtgct gtcgtagagg gagtacaggc
     4321 cgttgcggtc gcagtgccac aggtagaggg cgtagccggc caggttggca cagctgttgg
     4381 cggcgttgac gagttgcagc gtgagggtca ggggaatgcc ggtggcgacg gcgctggcgc
     4441 cggcaaagct gctgcggatg tcgctgcgca cgacgccggt gagggtcagg gcattgacga
     4501 cgccattgct gttggcattg gagccgtcgg ccgggtaggg gccggcggtc tcttcgggga
     4561 tcaccgagca ggtgctgctg gaggtgcttg agctgctgct ggacgaggtg ctggtgccga
     4621 cgctgccgag ccccacgtcg atgctgccgc cgcagccggc gaggggcgca gcggcgccgg
     4681 ccagcagggc gcgcagcagg gtgcggcgct gcggcgagct gcgcgagagg gcgtccaggt
     4741 catggagcag ggcgggggcg gcggggcgta caggggaggg catcgggacg gctccgggtc
     4801 ggaagtaagg ccctcggaag ggcgtgtgac ggatgcgaaa agtgtacgca gccgacccgg
     4861 tgtgccggag atgccggtgg gtaaaagcgg gtaaagatac gttggactgt gttcaggcgg
     4921 cggggggcgt gcgcagaatg gcaggcagcg cgggccgcca gccgaggctg tcgagcaggc
     4981 ggaggaagtc gccctccggg ggcgcgacga gatcgagggg cgcgccgctg cgtggatgga
     5041 tgaggcgcag gcgcgtgcac gccaggagca ttcgcgcgca gccgaagtgg tccgcgaaga
     5101 agcggttgtg gcggcccttg ccgaaggtgg cgtcgccgat cagggggtgg gcgatgtgtt
     5161 tgagatggcg gcggatctgg tggcgacgcc cggtgagcgg gcggacttcg agcagggcgt
     5221 agcggctgct cgggtagcgg tctacggcga agggcagctc gcaggtggcg aggcggacga
     5281 agtcggtgac ggcgggctgg gcggtgtcgc tgctggcgtc gccgcgccac tcggcctcgt
     5341 cgcggcggcg gctgaggggg tggtcgatgc gcccgctttg cggcggatga ccgcgcacca
     5401 cggccaggta ggtcttgtcg accgcctgat gttcgaagag ggcgctgccc agacgggcgg
     5461 tgtcccggtc cagggcgaag agcagcacgc ccgaggtgcc ccggtcgagg cggtgcaggg
     5521 gatggacgtg gcagcccagc tggtcgcgca gcatctgcac ggcgaagcgg gtttcgtggc
     5581 ggtcgagttc gctgcggtgg accagcaggc cggcgggttt atggatggcg acgaggtggt
     5641 cgtcccggta caggaggggc agcatgtcgg gcggtcggaa aggcgccgcg atcgggcact
     5701 cgggcagggg cacgcatcat accgcggcag gggtacggac cgctttcggg gcggaccggt
     5761 gacaaatgcc tttcttgcac gacaaatgga tgtactaaaa tgcggcgcgc tcgggcggag
     5821 ggtgcctgtc cgctggcaga acccgtccgt acggcgccgg cgctccagtg cgctgcgcac
     5881 cggtcgttaa tgattccgat gccggtcgcg gccggttcgg ccttcttttc ttcctgttcc
     5941 aggtcgtgtt catgagtctc ccgctgatct ttcccctgca cacccggcgg gcggcccgcc
     6001 ggctggccag tgcggggttc acgctgctgg aactgctggt ggtgatgacc atcatcggcc
     6061 tcctgg
//
LOCUS       DAOQPA010000162         5997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7809_length_5997_cov_19.011949, whole genome shotgun sequence.
ACCESSION   DAOQPA010000162 DAOQPA010000000
VERSION     DAOQPA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_5997_cov_19.011949"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKX01_15705"
     CDS             <1..672
                     /locus_tag="PKX01_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMZ77628.1"
                     /translation="YRAADKLKARTGVPDFRFAGLMGLDACDALTQALAEISGRPVPE
                     RIDRQRAQLQDAMVDCHFMLGFARVALAADPDLLGMQAHFLTGMGAEIVAAVSPAKAD
                     SLAELPIEQVTVGDLEDLELQARKRGAQLVIANSHAAETARRLGLPLMRAGFPQYDLV
                     GGYARTWVGYRGSRQALFDLANLMLSQHHELEPYRSVYWNESRDAGGSCEHHDTPSAA
                     TGLVH"
     gene            689..1114
                     /locus_tag="PKX01_15710"
     CDS             689..1114
                     /locus_tag="PKX01_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMZ77629.1"
                     /translation="MLKVAFATSDRVRVDQHFGAAESFALYEVTPDKATLVGVAEFPE
                     EAMDGNEGKLAAKVNFLEGCAAVYVMAIGASAIKQLLAKGIQPIRTNEVDAVFDLLAD
                     IGKAMTEGGVPWIDRALALQAKAASTDRFNSMEEEGWQG"
     gene            1111..1599
                     /locus_tag="PKX01_15715"
     CDS             1111..1599
                     /locus_tag="PKX01_15715"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HMZ77630.1"
                     /translation="MSSAHIEHRGVVRRVAGGKVFVAMETSGCASCDHGAGCGVGRLA
                     QGQDSTLLTVRADADYKAGDAVQVTLPERGMTLFAMLGYLFPAFVMMLGAALGMAVEG
                     SDAAAALGAIGGFLGAMLILRILISLMPDLMPTPQLSPIPLAPAASTLSPLEFHHDRH
                     DR"
     gene            1580..2038
                     /locus_tag="PKX01_15720"
     CDS             1580..2038
                     /locus_tag="PKX01_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HMZ77631.1"
                     /translation="MTATTADPILETDFVKEMARQMRALDTYGTYTGWSVEKILAPYV
                     LTKERKAEIPLIGDPDDETISRVKAFYNSIAVLIEKEAGLLAVPLIHLTHEGFGRALI
                     TVGRLVAVDKTLRDVHRFGFASLEKMKEEGDKILKGAVARITEHREVADA"
     gene            2053..2274
                     /locus_tag="PKX01_15725"
     CDS             2053..2274
                     /locus_tag="PKX01_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HMZ77632.1"
                     /translation="MSDVLTPEDLQALDKEVKKLKRIASEWASQMHDLVEDRLPAAYQ
                     EIPTLSQSTYEACKAWADANARLAAAQKG"
     gene            2280..2561
                     /gene="fdxB"
                     /locus_tag="PKX01_15730"
     CDS             2280..2561
                     /gene="fdxB"
                     /locus_tag="PKX01_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HMZ77633.1"
                     /translation="MSQITGLTRGGAEWTPQFVTELNQAKCIGCGRCYKVCPRNVLDL
                     VERELDDDDDSEDDNMMVMSIANAMDCIGCGSCGRVCPKDCYTYAPASV"
     gene            2561..2908
                     /locus_tag="PKX01_15735"
     CDS             2561..2908
                     /locus_tag="PKX01_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77634.1"
                     /translation="MALPVPDPALGARICEALRAQVAKLGLSIGDEPVWADAVFETRT
                     DPFSQEESRVGTWSGGLRYGNVTFFADGRIFAEYQVLLPLPDKADSYLESVQVWGSAE
                     RLRGDAVIAEFAK"
     gene            2916..3338
                     /locus_tag="PKX01_15740"
     CDS             2916..3338
                     /locus_tag="PKX01_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77635.1"
                     /translation="MSALPSPRLILLHKHGTSGRVRFLCLASGVLAFAPLPPLAALRD
                     EAYSPKVQVHPTAVVREAEVHLGMAEGTIQTEADFQAWVDTPAGDVPVLMGLFMGIEP
                     PFEVAARSQGRFIALTESRHLSDVERQLLRRAYEHVLG"
     gene            3459..3782
                     /locus_tag="PKX01_15745"
     CDS             3459..3782
                     /locus_tag="PKX01_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ77636.1"
                     /translation="MINLTPAAVKAVQRFIRGSETPVAGLRLVVSGGGCSGLQYGMKL
                     EAAKAEDDWELDIEGVKVLVDPMTFPMVDNVTIDFVDTLTHTGFKFDNPNASAQCACG
                     SSFSV"
     gene            3795..4691
                     /gene="nifU"
                     /locus_tag="PKX01_15750"
     CDS             3795..4691
                     /gene="nifU"
                     /locus_tag="PKX01_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HMZ77637.1"
                     /translation="MWEYSDKVREHFFNPRNSGPLEEANGIGDVGSIQCGDALRLMLK
                     VEPETEVIQDAHFQTFGCGSAIASSSALTEIIKGMTLDEALKVSNQDIADYLDGLPPE
                     KMHCSVMGREALQAAIANYRGEEWSDDHEEGALICKCFAVDAVMIEDVIKANNLNTVE
                     EVTFYTKAGGGCAACHEGIEEILARVKAELPGTGEVSPPPACPATPEAPKPPAAKLTL
                     VQKIKKIEEVLESVRPMLQRDHGDVELADVQGKKIYVHLKGACSGCMMEAATLGGIQQ
                     KMIEALGELVQVLPASHMPVEA"
     gene            4735..5943
                     /gene="nifS"
                     /locus_tag="PKX01_15755"
     CDS             4735..5943
                     /gene="nifS"
                     /locus_tag="PKX01_15755"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HMZ77638.1"
                     /translation="MEPIYLDNNATTRVDPAVVDAMLPFFTEHFGNASSIHAFGSKVG
                     AALKKARGQVQALIGAEHDSEIIFTSCGTESDSTAILSALKAQPDRKTVITTVVEHPA
                     ILTLCEHLEKEGYTVHRLTVDSKGRLDLEAYKALLSDDVAIVSVMWANNETGTLFPVV
                     EMAELASAKGIMFHTDAVQAVGKVAIDVKSSKIDMLSLSGHKLHAPKGIGVLYLRRGC
                     RFRPLLRGGHQERGRRAGTENSASIVGLGQACELALANLDKENTQVKALRDKLEQGIL
                     SQISHCFPTGDVDNRLPNTSNIAFEYIEGEAILLLLNKLGIACSSGSACTSGSLEPSH
                     VMRAMGIPYTAAHGTIRFSLSVDNTEAEIDRVIAAVPPIIAQLRKLSPYWGENGPAAN
                     PAETFAPTYA"
BASE COUNT         1074 a         2204 c         1799 g          920 t
ORIGIN      
        1 taccgcgccg ccgacaagct caaggcccgc accggcgtgc ccgacttccg ctttgccggc
       61 ctgatgggcc tggatgcctg cgacgccctc acccaggccc tcgccgagat ctccggccgt
      121 ccggtgcccg aacgcatcga ccgccagcgc gcccagctgc aggacgccat ggtggattgc
      181 cacttcatgc tcggctttgc ccgcgtcgcg ctggccgccg atccggacct gctgggcatg
      241 caggcccact tcctcaccgg catgggcgcc gagatcgtcg ccgcggtgag cccggccaag
      301 gccgacagcc tcgccgaact gcccatcgag caggtgacgg tgggcgacct ggaagacctg
      361 gagctgcagg cccgcaagcg cggcgcccag ctcgtcattg ccaattccca tgccgccgag
      421 accgcgcgcc gcctcggtct gccgctgatg cgcgcgggct ttccgcagta cgacctggtg
      481 ggcggctacg cccgaacgtg ggtcggctac cggggctcgc ggcaggcact gttcgatctc
      541 gccaacctga tgctgagcca acaccatgaa ctcgaaccct atcgatccgt gtattggaac
      601 gagtcccggg acgccggcgg ctcctgcgaa caccatgaca ccccgtcggc tgcaactggt
      661 ctggtccatt aagaaggagc ccgctgccgt gctcaaggtc gcttttgcca cgtccgaccg
      721 cgtccgggtc gaccagcact tcggcgctgc cgagtccttc gccctctacg aggtcacgcc
      781 cgacaaggcc accctcgtcg gcgtggccga gttccccgaa gaggccatgg acggcaacga
      841 aggcaagctc gccgccaagg tgaacttcct ggaaggctgc gccgcggtct acgtgatggc
      901 catcggcgcc tcggcgatca agcagcttct cgccaagggc atccagccca tccgcaccaa
      961 cgaggtggac gccgtgttcg acctgctcgc cgacatcggc aaggccatga ctgaaggcgg
     1021 cgtgccctgg attgaccggg ccctcgccct gcaggccaag gccgcctcga cggaccgttt
     1081 caactcgatg gaagaggagg gctggcaggg atgagcagcg cacacatcga acaccgcggc
     1141 gtggtacgcc gtgtcgccgg cggcaaggtc ttcgtcgcca tggaaacctc cggctgcgcc
     1201 tcctgcgacc acggcgccgg ctgcggcgtg ggccgcctgg cccagggcca ggacagcacc
     1261 ctgctgaccg tgcgcgccga cgccgactac aaggccggcg atgccgtcca ggtcaccctg
     1321 cccgagcggg gcatgaccct cttcgccatg ctgggctacc tcttcccggc cttcgtgatg
     1381 atgctcggcg ccgccctcgg catggccgtg gagggcagcg acgcggctgc cgccctcggc
     1441 gccatcggcg gcttcctggg cgccatgctg atcctgcgca tcctcatcag cctgatgcct
     1501 gacctgatgc cgacgcccca gctgtccccg attcctctgg cccccgcggc ctccaccctt
     1561 tcccctctgg agtttcatca tgaccgccac gaccgctgat cccatcctcg agacggactt
     1621 cgtcaaggag atggcccgcc agatgcgtgc cctcgacacc tacggcacct acaccggctg
     1681 gagcgtcgag aagatcctcg cgccctacgt cctcaccaag gaacgcaagg ccgagatccc
     1741 cctcatcggc gacccggacg acgaaaccat ctcccgcgtg aaggccttct acaactcgat
     1801 cgccgtgctc atcgagaagg aagccggcct cctcgccgtg cccctgatcc acctcaccca
     1861 cgaaggcttc ggccgcgccc tcatcaccgt gggccgtctg gtggcggtgg acaagaccct
     1921 gcgcgacgtc catcgcttcg gcttcgccag cctggagaag atgaaggaag agggcgacaa
     1981 gatcctgaag ggcgccgtgg cccgcatcac cgaacaccgc gaagtcgccg acgcctgagt
     2041 ccgggagcca tcatgtccga cgtcctcacc cccgaagacc tgcaggccct cgacaaggaa
     2101 gtgaagaagc tcaagcgcat cgcttccgag tgggcctcgc agatgcacga tctggtggaa
     2161 gaccgcctgc cggccgccta ccaggagatt cccaccctgt cccaatccac ttacgaggcc
     2221 tgcaaggcct gggccgacgc caacgcccgc cttgccgccg cccagaaagg ctgatcacca
     2281 tgagccagat caccggtctc acccgcggcg gcgccgaatg gacgccccag ttcgtcaccg
     2341 agctcaacca ggccaagtgc atcggctgcg gccgctgcta caaggtctgt ccgcgcaacg
     2401 tgctcgacct ggtcgagcgc gagctcgacg atgacgacga ctccgaagac gacaacatga
     2461 tggtcatgtc catcgccaac gccatggact gcatcggctg cggctcctgc gggcgcgtct
     2521 gtcccaagga ctgctacacc tacgcgccgg cgtccgtgtg atggccctgc cggtccccga
     2581 tccggcgctg ggggcccgca tctgcgaggc cctgcgcgcc caggtggcca agctgggcct
     2641 gtccatcggc gacgagccgg tgtgggccga cgccgtcttc gagacgcgca ccgatccctt
     2701 ctcccaggaa gagagccggg tcggcacctg gagcggcggg ctgcgctacg gcaacgtcac
     2761 cttctttgcc gacgggcgca tctttgccga gtaccaggtg ctgctgcccc tgccggacaa
     2821 ggccgacagc tacctggagt ccgtccaggt gtggggcagc gccgaacgtc tgcggggaga
     2881 cgcggtgatc gccgagtttg ccaagtgagc tccgcgtgtc ggccttgcct tcgccgcgcc
     2941 tgatcctgct gcacaagcac ggcaccagcg ggcgggtgcg cttcttgtgc ctcgcctcgg
     3001 gcgtgctcgc ctttgcgccc ctgccgcccc tggcagccct gcgcgacgag gcctacagcc
     3061 ccaaggtgca ggtgcatccc accgcggtgg tgcgcgaggc cgaggtgcat ctgggcatgg
     3121 ccgaaggcac catccagacc gaggcggatt ttcaggcctg ggtggatacg cctgccggcg
     3181 atgtgcccgt gctgatgggc ctcttcatgg gcatcgagcc gcccttcgag gtggccgcgc
     3241 gcagccaggg gcgcttcatc gccctcaccg aatcacgtca cctcagcgac gtggagcgcc
     3301 agctcctgcg ccgcgcctac gaacacgtgc tgggctgatt gtcgtaaata cgacaacggc
     3361 ggtctttgaa acaagccagt caattcaatc gcttaccgcg atgcagaaga tggcacaggg
     3421 cttgcgaggt tctcttcatc ctaactggga gaaccaacat gatcaatctg acccccgcag
     3481 cagtgaaagc cgtccaacgc ttcatccgtg gctccgaaac ccccgtcgcc ggcctgcgcc
     3541 tggtggtctc gggcggtggc tgctccggcc tccagtacgg catgaagctc gaagccgcca
     3601 aggccgagga cgattgggaa ctggacatcg aaggtgtcaa ggtcctggtg gacccgatga
     3661 ccttcccgat ggtggacaac gtcaccatcg actttgtcga caccctgacc cacaccggtt
     3721 tcaagttcga caaccccaac gccagcgccc agtgcgcctg cggttcttcc ttctcggtct
     3781 aaggagcgcc agccatgtgg gaatactctg acaaggtccg cgaacacttc ttcaacccgc
     3841 gcaactccgg ccccctggaa gaagccaacg gcatcggcga cgtgggctcc atccagtgcg
     3901 gcgacgccct gcgcctgatg ctcaaggtcg aacccgaaac cgaagtcatc caggacgcgc
     3961 acttccagac cttcggctgc ggctccgcca tcgcctcctc gtcggccctg accgagatca
     4021 tcaagggcat gaccctcgac gaagccctca aggtctccaa ccaggacatc gccgactacc
     4081 tggacggcct gccccccgaa aagatgcact gctccgtgat gggccgcgaa gccctgcagg
     4141 ccgccatcgc caactaccgc ggcgaggaat ggtccgacga ccacgaagaa ggcgccctca
     4201 tctgcaagtg cttcgccgtg gatgcggtga tgatcgaaga cgtgatcaag gccaacaacc
     4261 tcaacaccgt cgaggaagtc accttctaca ccaaggccgg cggtggctgc gccgcctgcc
     4321 acgaaggcat cgaggagatc ctcgcccgcg tcaaggccga gctgcccggc accggcgaag
     4381 tgtcgccccc gcccgcctgc ccggccacgc cggaagcgcc caagccgcct gccgccaagc
     4441 tcaccctggt gcagaagatc aagaagatcg aggaagtcct cgaatccgtc cgccccatgc
     4501 tccagcgcga ccacggcgac gtggaactgg ccgacgtgca gggcaagaag atctacgtgc
     4561 acctcaaggg cgcctgctcc ggctgcatga tggaagcggc gaccctcggc ggcatccagc
     4621 agaagatgat cgaggccctg ggcgaactgg tgcaggtcct gccggccagc cacatgcccg
     4681 tcgaagcctg acatcccgac atccccccac agagaatctg acaaggactg cgccatggaa
     4741 ccgatctacc tggacaacaa cgccaccacg cgggtcgacc ccgccgtggt ggacgcgatg
     4801 ctgcccttct tcaccgagca cttcggcaac gcctcgtcca tccatgcctt cggcagcaaa
     4861 gtgggcgccg ccctcaagaa ggcacggggc caggtgcagg ccctcatcgg cgccgagcac
     4921 gattcggaga tcatcttcac ctcgtgcggc accgagtccg actccaccgc catcctctcc
     4981 gccctcaagg cccagccgga ccgcaagacg gtgatcacca ccgtcgtcga gcacccggcc
     5041 atcctcaccc tctgcgaaca tctggagaag gagggctaca ccgtccaccg cctcacggtg
     5101 gatagcaagg gccgcctcga cctggaagcc tacaaggccc tgctcagcga cgacgtggcc
     5161 attgtctcgg tgatgtgggc caataacgag accggcaccc tcttccccgt ggtggagatg
     5221 gccgaactcg cctcggccaa gggcatcatg ttccacaccg acgccgtgca ggccgtgggc
     5281 aaggtggcca tcgacgtcaa gagcagcaag atcgacatgc tctccctgtc cggccacaag
     5341 ctccacgccc ccaagggcat cggcgtgctc tacctgcgcc gcggctgccg cttccgcccg
     5401 ctgctgcggg gcggccacca ggaacgcggg cgccgcgccg gcaccgagaa ctccgcctcc
     5461 atcgtcggcc tcggccaggc ctgcgaactg gccctcgcca acctggacaa ggaaaacacc
     5521 caggtcaagg ccctgcggga caagctggag cagggcatcc tgagccagat ctcccactgc
     5581 ttccccaccg gcgacgtgga caaccgcctg cccaacacca gcaacatcgc cttcgaatac
     5641 atcgaaggcg aagccatcct gctgctcctc aacaagctgg gcatcgcctg cagctccggc
     5701 tccgcctgca cctcgggctc cctggagccc tcccacgtca tgcgcgccat gggcatcccc
     5761 tacaccgccg cccacggcac catccgcttc tccctgtcgg tggacaacac cgaagccgag
     5821 atcgaccgcg tcatcgccgc ggtaccgccg atcatcgccc agctgcgcaa gctgtccccc
     5881 tactggggcg aaaacggccc ggccgccaac ccggcggaga cgtttgcgcc gacctacgcc
     5941 tgacgggggg ttttacggaa aacgggacgg ccggatcggg agatccggcc gttttgt
//
LOCUS       DAOQPA010000163         5935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_7924_length_5935_cov_14.499320, whole genome shotgun sequence.
ACCESSION   DAOQPA010000163 DAOQPA010000000
VERSION     DAOQPA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_5935_cov_14.499320"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKX01_15760"
     CDS             <1..1137
                     /locus_tag="PKX01_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ77639.1"
                     /translation="PAPADDIHTEQASQTPAADARSPVMCPSPLSLEPLNAPPTGNSQ
                     PPAPPPFTTAPQPIHPPPPGMAPTTPATTPHSHARWAWIAGLTVAGLAGLAGWVWSET
                     RPTGLSPIQLAQARGAEASTEVQPPVSAPPPETPPETLPAAPPAVARSRTTSPAARPP
                     SPARTDPPDSDAHAALRFEPRPMPVASPQPDLLRAHDAYTRGDLTAAHQHYQAALSLD
                     PTNPDAHDGLGALALHSGLHVEAARHFRLALAARPTDSAALAGLASLPGNDNRQAKSR
                     LRSSLDADAGDATAQFALGTVLARQQRWAEAQHAFFRARSLAPEDPDIAYNLAVSLDH
                     LRQPGPAAAHYRLALQLAERHLAGFDRAACAARIDALAAPDNAP"
     gene            1134..2810
                     /locus_tag="PKX01_15765"
     CDS             1134..2810
                     /locus_tag="PKX01_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HMZ77640.1"
                     /translation="MNGQPLGQALLQRGLISADQLAIALREQERSHEAIGRVLVRLGF
                     LSEATLRDALSHALGRRSVDLDTLVPDPAALTLLPRDLACRLNVLPVSLDSTSRVLTV
                     AAARIGDPILLDSLQAQLPAGLHIEPVLAAESQIARAIDQLYGHALSIDGILHEIEAG
                     AAGPVEQAQPVVRLVDALLVDAVKSDASDIHFEPEPGFLRIRYRIDGLLRQIRALHTS
                     YWPAMAVRIKVMAGLNIAETRMPQDGRISLHVNGRPVDFRVSTHPTLHGENIVLRVLD
                     RQKGIVPLDRLGLADTQLDTLKLMIARPEGLILVTGPTGSGKTTTLYSILNHINAEGI
                     HIMTLEDPVEYPMPLIRQTAVAEAAHLDFGNGIRAMLRQDPDVILVGEIRDTDTARMA
                     LQAAMTGHQVYSTLHTHSAVGALPRLLDLGVAADILAGHLVGIVAQRLLRRLCPHCRE
                     ARPAEDYELHLLAQPADAPRPRLHHPRGCPQCQFQGYRGRMAIMELLRIDHRLDELVA
                     RRASPRELLAAARDQGFRSLAEDGSRRVLEGHTSLEELARVVDLTDRMRP"
     gene            2807..4009
                     /locus_tag="PKX01_15770"
     CDS             2807..4009
                     /locus_tag="PKX01_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMZ77641.1"
                     /translation="MTRFRYRAADAHGQLREGELEAHHVDDLDLRLRRLGLDLIRATP
                     ASRTPRGGRRGVPRRELINFCFHLEQCQRAGVPILESLADMRDSTPHPVLRDTLGALV
                     DGIQGGLSLSRALERHPALFDGVFVSLVRAGEQAGQLPTVLHALAEGLKRDDELAAFA
                     RRIAIYPAIVGTVIAAALVVALLYVVPELARLFHATGQPLPLQTRLLIGLSNLLIDHG
                     RLLAVLALAAVAALRFALATRPDFRRWYDGAQLALPVLGEIRRKIILARFTALFAMMY
                     GAGITIVDALAAAEGVVGNTALRAALQRIADAIGDGRRVSDAFADTPAFPPLVARMLR
                     VGEHTGALDTALANVGYFYERDVREAIARVQASLEPALTLLLGGLLLWVMSAVLLPVY
                     DLATRMKL"
     gene            4012..5436
                     /locus_tag="PKX01_15775"
     CDS             4012..5436
                     /locus_tag="PKX01_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77642.1"
                     /translation="MALCLLHLCADGATAYLRTTGAYRASARFGQADAAFSAWLGAHP
                     RMTLRVMADLPDEQFLIDALPILRGQERHQLIERRRSRLFASTPFVGIRPLGRADGRE
                     TLLFHALTRPAALQPWLDALRHAGARLDGLHSVADAMAALRPHLPSGAPTCAIAGLTH
                     TGLRLTALVDKHPQWSRLVPATAVTDLSTDLPNICNAARDHLVARRLLHPEHPLPVVL
                     LPGLETFAGALGAGLQAIAPSATELARRCRLPPPGPHTDALPYILYGAGRRGLPQFVP
                     PDRLTARPLRAVDQAILLAGATAAAIGAAVAIKFMADTRALSDTDARLTAEVVATEDR
                     LSRVNAALPPPPAGDPLAATQSLSRLQDARNWPTRLLDRLSDLLEAEPDSELKSVEWQ
                     LPPDGPPVAVAEIALPAALADTPPALAARLDRLRAAVISAGYVQASLERVPDELDTTR
                     SVRLRPAPEAQPRVVVRFSPAPPP"
     gene            5433..>5935
                     /locus_tag="PKX01_15780"
     CDS             5433..>5935
                     /locus_tag="PKX01_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77643.1"
                     /translation="MSHLPLIRPGPAHRSVAAALALALLGGGAAYSTASRHADAEARL
                     LQTQHALTDVRTRLQVAEDTLAARQKQLAAAAQLRAQGLSAGPDRPAWLKAVRAELQA
                     QHLASLDVELGPHRPLDGAADFDASPLTLRGPLAHEGQLLRLLDVLDRQPGTLLLPRR
                     CRVSRLDT"
BASE COUNT          825 a         2586 c         1728 g          796 t
ORIGIN      
        1 cccgccccag cggacgacat ccatacggag caggccagcc agacgcccgc cgctgacgcc
       61 cgcagccctg tcatgtgccc ctcgcccctg agcctcgaac ccctcaacgc ccccccaact
      121 ggcaacagcc agccccctgc gccgccgccc ttcacgaccg cacctcagcc catccacccg
      181 ccgcccccgg ggatggcccc cacgacaccc gccaccacgc cacactccca cgcccggtgg
      241 gcctggatcg ccggcctgac ggtcgccgga ctggcggggc ttgccgggtg ggtgtggtcg
      301 gagacccgcc cgaccggcct gtcccccatc cagcttgccc aagcacgcgg cgccgaagcc
      361 tcgaccgaag tgcagccccc tgtgtccgcc ccgccaccgg aaacaccccc ggaaaccctg
      421 cctgcggcac cacccgccgt ggcccggtct cgcaccacgt cgccggccgc gcggccaccc
      481 tcccccgccc gcaccgaccc gcccgattcg gacgcgcacg ccgcactgcg cttcgagccc
      541 cgcccgatgc cggtggcctc gccacagccc gaccttctcc gcgcccacga cgcctacaca
      601 cgcggcgatc tcaccgcggc gcaccagcac taccaggccg ccctgtccct cgatcccacc
      661 aacccggacg cccatgacgg gcttggcgcc ctcgccctac acagcggtct gcacgtcgag
      721 gccgcccgcc acttccgcct ggccctcgcc gcccgcccta cggacagcgc agccctcgcc
      781 gggcttgcca gtctgcccgg caacgacaac cgccaggcca aaagccgcct gcgcagcagc
      841 ctcgacgcgg acgccggcga cgccaccgcc cagttcgccc ttggcacggt gctggcccgc
      901 cagcagcgct gggccgaggc ccagcacgcc ttcttccgtg cccgcagcct cgcgcccgaa
      961 gatccggaca ttgcctacaa cctggcggtc agcctcgatc accttcgcca gcccggtccc
     1021 gccgcagcgc attaccgcct cgcgctgcaa ctggccgaac ggcacctggc gggattcgac
     1081 cgggccgcct gcgccgcacg catcgacgcc ctggcagccc cggacaacgc accgtgaacg
     1141 gacaacccct cggccaggcg ctcctgcaac ggggcctgat cagtgccgat caactcgcca
     1201 tcgccctgcg ggagcaggag cgcagccacg aagcgatcgg tcgcgtgctg gtccgcctgg
     1261 gattcctgtc ggaagccacg ctgagggacg ccctgtccca tgccctgggc cggcgcagcg
     1321 tggatctcga caccctggtg cccgaccctg ccgccctcac cctgctgccc cgcgacctgg
     1381 cctgtcgcct gaacgtcctc cccgtgagcc tggacagcac gagccgggtg ctcaccgtgg
     1441 ccgctgcgcg catcggcgac ccgatcctgc tggacagctt gcaggcccag ctccccgccg
     1501 gcctgcacat cgagcccgtg ctggcggccg aatcgcaaat cgcccgggcc attgaccagc
     1561 tctacggcca cgcgctgtcc atcgacggca tcctccacga gatcgaggcc ggcgcggcgg
     1621 gcccggtgga gcaggcccag ccggtcgtcc gcctggtgga cgcgctgctt gtggacgcgg
     1681 tcaagtccga cgcctccgac atccacttcg aacccgaacc cggcttcctg cgcatccgct
     1741 accgcatcga cggcctgctg cgccagatcc gtgccctgca cacgtcctac tggcccgcca
     1801 tggccgtccg catcaaggtc atggcgggcc tcaacatcgc cgagacccgc atgccccagg
     1861 acggccgcat ctccctgcac gtcaacggcc gtcccgtcga tttccgcgtc tccacccacc
     1921 ccacgctgca cggcgagaac atcgtcctgc gcgtcctcga ccgccagaag ggcatcgtcc
     1981 ccctcgaccg tctcggcctg gccgacaccc agctcgacac cctcaagctg atgatcgccc
     2041 gtcccgaggg cctcatcctc gtcaccggcc ccaccggcag cggcaagacc accacgctgt
     2101 attccatcct caatcacatc aacgccgagg gcatccacat catgaccctc gaggatccgg
     2161 tggagtaccc catgcccctg atccgccaga ccgccgtcgc cgaggccgcc cacctggatt
     2221 tcggcaacgg catccgcgcc atgctgcgcc aggatccgga cgtgatcctg gtcggcgaga
     2281 tccgcgatac cgacaccgcc cgcatggccc tgcaggccgc catgaccggc caccaggtgt
     2341 actccacgct gcatacccac tccgccgtcg gcgccctgcc gcgcctgctt gacctgggcg
     2401 tcgcggccga catcctggcc ggccacctcg tcggcatcgt cgcccagcgc ctgctgcgcc
     2461 gcctgtgccc ccactgccgc gaagcccgtc cggcagaaga ctacgaactg cacctgctcg
     2521 cccagcccgc cgacgccccc cgcccccggc tccaccaccc ccgcggctgc ccccaatgcc
     2581 agttccaggg ctaccgcggg cgcatggcca tcatggaact gctgcgcatc gaccaccgcc
     2641 tcgacgaact cgtcgcacga cgcgccagcc cacgcgagct cctcgccgcg gcccgcgacc
     2701 agggcttccg gagcctcgcc gaagacggca gccggcgggt cctggaaggc cacaccagtc
     2761 tcgaggagct cgcccgcgtg gtggacctca ccgaccgcat gcgcccatga cccgcttccg
     2821 ctaccgcgcc gcggacgccc acggccaact ccgcgaaggc gaactggaag cccaccatgt
     2881 ggacgatctg gacctgcgcc tgcgccgcct cggcctcgac ctgatccgcg ccaccccggc
     2941 cagccggacg ccccggggag gacgccgagg cgtgccgcgg cgcgaactca tcaacttctg
     3001 cttccacctg gagcagtgtc agcgcgcggg cgtccccatc ctcgaaagcc tcgccgacat
     3061 gcgcgacagc acgccccacc cggtgctgcg cgacaccctc ggcgcccttg tcgacggtat
     3121 ccagggcggc ctcagcctgt cccgcgccct cgaacgccac cccgcgctct tcgacggcgt
     3181 ctttgtcagc ctggtccgcg ccggcgaaca ggccggacaa ctgcccaccg tccttcacgc
     3241 cctcgccgag gggctcaagc gtgacgacga actcgccgcc ttcgcgcgcc ggatcgccat
     3301 ctaccccgcc atcgtcggca ctgtcatcgc cgccgccctc gtcgtcgccc tgctttacgt
     3361 cgtgccggaa ctcgcccgcc tgttccatgc caccggccag cccctgcccc tgcagacacg
     3421 cctgctcatc ggcctgtcga acctcctgat cgatcacggc cgcctgctgg cagtgctggc
     3481 gctcgccgcc gtcgccgccc tgcgcttcgc cctcgccacc cgccccgact tccgccgctg
     3541 gtacgacggc gcccaactgg cgctgccggt ccttggcgag atccggcgca agatcatcct
     3601 cgcccgcttc accgccctct tcgccatgat gtacggcgcc ggcatcacca tcgtggacgc
     3661 cctcgccgcg gccgagggcg tggtcggcaa caccgccctg cgcgcggccc tgcagcgcat
     3721 cgccgacgcc atcggtgacg gccggcgggt ctcggacgcc ttcgccgaca cgcccgcctt
     3781 tccgcccctc gtcgcccgca tgctgcgtgt cggcgagcac accggcgccc tcgacacggc
     3841 cctcgccaac gtaggctatt tctacgaacg ggacgtgcgc gaagccatcg cccgcgtgca
     3901 ggccagcctc gagcccgccc tcaccctgct cctcggcggc ctcctcctgt gggtcatgag
     3961 cgccgtgctg ctaccggtct acgatctcgc gacccgcatg aagctctgat catggcgctc
     4021 tgcctcctcc acctgtgcgc ggatggcgcc accgcctatc tccggacgac cggtgcatac
     4081 cgggccagcg cccgtttcgg gcaggccgac gccgcgttct cggcctggct cggcgcccac
     4141 ccacgcatga cactgcgggt gatggccgat ctgccggacg agcagtttct catcgacgca
     4201 ctgcccatcc tgcggggtca ggagcgccac cagctcatcg aacggcggcg cagccggctc
     4261 tttgcctcga cgccctttgt cggcatccgc ccgcttggcc gcgcggacgg gcgggaaacc
     4321 ctgctgttcc acgcactcac ccgccccgcc gcattgcagc cctggctgga tgccctgcgc
     4381 cacgccggcg cccggctcga tggtctgcac agcgtcgccg acgcaatggc cgcgctgcgg
     4441 ccccacctgc cgtccggcgc cccgacctgt gccattgcgg gtctgacgca caccggtttg
     4501 cgcctcacgg ccctcgtcga caaacatccg cagtggagcc gcctggtgcc agccaccgca
     4561 gtcaccgatt tgtccaccga tctcccgaac atctgcaatg ccgcccgcga ccatctcgtg
     4621 gcccgtcggc tgctccaccc cgaacatccc ctcccggtcg tgctgctgcc cggcctggag
     4681 accttcgcgg gtgcgctggg agccggcctg caggccatcg cgccgtcggc caccgagctc
     4741 gcccgccgct gccgcctgcc gccccccggc ccgcacaccg acgcccttcc ctacatcctc
     4801 tacggtgcag gccgccgcgg gctcccccaa ttcgtcccgc cagaccggct gaccgcccgc
     4861 cccctgcgcg ccgtcgacca ggccatcctg ctggccggcg ccaccgccgc cgccatcggc
     4921 gcggccgtcg ccatcaagtt catggccgac acccgggcgc tcagcgacac cgacgcccgc
     4981 ctgacggccg aggtcgtcgc gaccgaagat cgcctcagcc gcgtgaacgc cgcgttgcct
     5041 ccgcccccag ccggcgaccc cctcgccgcc acgcaaagcc tgagccgcct tcaggacgcc
     5101 cggaactggc cgacaaggct gctcgaccgg ctgtccgacc tgcttgaggc cgagcccgac
     5161 agcgaactga agtccgtgga gtggcagctc ccgcctgacg gaccccccgt ggcggtggcg
     5221 gagattgccc tgccggcggc cctggccgac acgccccccg cgcttgccgc gaggctggac
     5281 cgcctccgcg ctgcggtgat ctcggccggc tacgtccagg ccagcctcga acgcgtgccc
     5341 gacgaactgg acaccacccg gagcgttcgg ctccgaccgg cgccggaagc acaaccgcgg
     5401 gtcgtggtcc gcttcagccc cgcgccgccg ccatgagtca ccttcccctg atccggcccg
     5461 gccccgccca ccgcagcgtg gcagcggccc tggccctcgc cctcctcggc gggggcgcgg
     5521 cctacagcac ggccagccgc cacgcggacg cggaggcccg cctcctgcag acgcagcacg
     5581 cgctcaccga cgtccgcacg cgcctccagg tcgccgaaga caccctcgca gcccgacaga
     5641 agcagctcgc cgccgccgcc caactgcgcg cacaggggct gtccgccggg ccggaccggc
     5701 ccgcctggct caaggccgtg cgtgcggaac tgcaagccca gcacctcgcc tccctggacg
     5761 tcgaactggg gccccaccgc ccgctcgacg gcgcggcgga tttcgatgcc tcgccgctga
     5821 ccttgcgggg cccccttgcc cacgaaggcc agctcctcag gctgctcgac gtcctcgacc
     5881 gacagccggg caccctgctc ctgccccgcc gctgtcgcgt cagccgtctc gacac
//
LOCUS       DAOQPA010000164         5663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8598_length_5663_cov_17.667439, whole genome shotgun sequence.
ACCESSION   DAOQPA010000164 DAOQPA010000000
VERSION     DAOQPA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8598_length_5663_cov_17.667439"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /gene="cphA"
                     /locus_tag="PKX01_15785"
     CDS             complement(<1..1653)
                     /gene="cphA"
                     /locus_tag="PKX01_15785"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMZ77644.1"
                     /translation="MEVSRIRALRGPNLWSRHTSIEAIVSCADGECDLDGLPGYEARL
                     RNLFPEMGFLKPEGHHGTISLAHALELAALGLQSSAGCPVTFSRTSATIEKGVFQVVV
                     EYTEEKVGRLAFDLAVDLCTAALEERNFDLADALARLRELDEDVRLGPSTGSIVQAAV
                     ARGIPFRRLTEGSLVQFGWGSQQRRIQAAETDLTSAVAESIAQDKELTKNLLRAAGVP
                     VPFGRSVTDAEDAWAAAQEIGGLVVVKPRDGNQGKGVAVKLRSREEVMAAYTVAAEIS
                     DDVMVERYLPGHDYRLLVVGSKLIAAARRDPPLVIGDGVRTIRQLVEHVNSDPRRGVG
                     HATSLTKIRFDEIALATLTKQGYEADSVPPKGKRVILRNNANLSTGGTATDVTDDVHP
                     EVAERAIAAAKNIGLDICGVDVVCDSVLEPLEAQSGGIVECNAAPGLRMHLQPSYGKG
                     RPVGEAIVGNMFAPGENGRIPVVAVAGTNGKTTTVRLTAHLLGVTGKRVGMTNSDGVY
                     VGARRIDTGDCSGPRSARNILMHPDVDAAVFETARGGVLREGL"
     gene            complement(1730..3922)
                     /gene="cphA"
                     /locus_tag="PKX01_15790"
     CDS             complement(1730..3922)
                     /gene="cphA"
                     /locus_tag="PKX01_15790"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMZ77645.1"
                     /translation="MTNKTIEFLNVFHLRGPNIWTYRAVLEAWVDIHDLEDCPSNVIP
                     GFYERLTALLPSLIEHRCSIGERGGFLKRLRDGTWPGHILEHVTLELQNLAGMSGGFG
                     KARETSDRGVYKVVIRAWQKDVSLAALHMGRDLLMAAIEGRPYDVDAAVRRLRDLVDK
                     YMLGPSTRCIVEAAADKDRRIPYIRLSEGNLVQLGYGARQRRIWTAETDRTSAIAESI
                     SREKDLTKSLLESCGVPVPEGRIVTSPEDAIEAAGDIGYPVVVKPSDGNHARGVFTNI
                     STPDEVAKAFPLSLAQGSEVIVERFVRGSEHRLLVVGGRMVAANKGDTVSVVGDGKHT
                     IDQLIDLQINSDPRRGDEHEFPLYRITLDRFPAAQMEVERQGYTGSSIPPDGQDVMIV
                     RTSNMAFDVTDQVHPDTAELACLAARIVGLDIAGVDLVCEDISLPLGAQKGAIVEVNA
                     GPGLLMHIKPGVGEPRPVGEAIVAHLFEHDADSRIPVVGVTGSRGKTPVARLVTHLLR
                     LSGQYTGLASSEGVFLDGRQVETPARTNWDAAERVLMNRSVDVAVFENGCRSILSDGT
                     VYDRCLVGVVTNVDAPLHYGEFYMDAPEKVWNVFRTQVDLVLPQGAAVLNAEDPAVVE
                     MAELSDGEVIFYGVDGQAPAIAQARSEGKRTVYVSDGAIVLATGDKAEAVARLNKIAM
                     LRNGAGERPGVLSNLLAAVAAAWALGVAPALIRAGVKPYDPNAPLRND"
     gene            4202..>5663
                     /locus_tag="PKX01_15795"
     CDS             4202..>5663
                     /locus_tag="PKX01_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMZ77646.1"
                     /translation="MTSQPALPPRASHNAGTDAYSDYLGPAVPADWRQRLDPTLEAGE
                     KVLASVVLDLDTALRFRGGLLAVTDRRLLARAQDDDTWQAWTYRDGLHLRHRDHAGVA
                     ALELMDDGGRLAGWRFTLAQDPAARNLADRFHAQLDCVLSGRAPTPPEPLTCPTCDAP
                     LPPGTDECPTCAREIHTPPSTWTLFRLWRFARPYKSQLLSGFILTLLATGATLIPPYL
                     TMPLMDEILIPFQNGTPIDTHRVTLILGGLLVAALFAWGLGWAKTYILALVSERIGAD
                     LRTTTYEHLQGLSLEYFGGKRTGDLMARIGAESDRICLFLSMNLLDFATDVLMIAMTA
                     AILVSINPWLALVTLVPLPFIAWLIHIVRDKLRYGFEQVDRVWSEVTSVLADTIPGIR
                     VVKAFAQEKREVARFREANARNLAVNDRVNKVWSLFSPTVTLLTEVGLLVVWGFGIWL
                     VSERDVTVGTLAAFLAYISRFYTRLDSMSRIVSVTQK"
BASE COUNT          820 a         2015 c         1896 g          932 t
ORIGIN      
        1 gaggccttcc cgcagcacgc cgccgcgggc ggtctcgaac accgccgcgt ccacgtccgg
       61 atgcatgagg atgttgcggg cgctgcgcgg gcccgagcag tcgcccgtgt caatgcgccg
      121 cgcgcccacg tagacgccgt ccgagttggt catgcccacg cgcttgccgg tgacgcccag
      181 caaatgcgcg gtgagacgca ctgtggtggt cttgccgttg gtgcccgcca ccgccaccac
      241 cgggatgcgg ccgttctcgc cgggcgcgaa catgttgccg acgatggcct cgcccaccgg
      301 gcggcccttg ccgtaggacg gctgcaggtg catgcgcagg ccgggcgcgg cattgcattc
      361 gacgatgccg ccgctctggg cttcgagggg ctccagcacg ctgtcgcaca ccacgtccac
      421 gccgcagatg tcgaggccga tgttcttggc cgcggcgatg gcccgctcgg ccacctcggg
      481 gtggacgtcg tcggtcacgt cggtggcggt gccgccggtg gacaggttgg cgttgttgcg
      541 caggatcacc cgcttgccct tgggcggcac ggaatccgct tcgtaaccct gcttggtgag
      601 ggtggccagg gcgatctcgt cgaagcggat cttggtgagg gaggtggcat gaccgacgcc
      661 gcggcgcggg tcgctgttca cgtgctccac cagctggcgg atggtgcgca cgccgtcgcc
      721 gatcaccagg ggcggatcgc ggcgggccgc ggcgatgagc ttgctcccca ccaccagcag
      781 gcggtagtcg tggccgggca ggtagcgctc gaccatcacg tcatcgctga tctcggcggc
      841 cacggtgtag gccgccatca cctcctcacg gctgcgcagc ttgaccgcca cgcccttacc
      901 ctggttgccg tcgcggggct tgaccaccac caggccgccg atctcctgag ccgcggccca
      961 ggcatcctcg gcgtcggtca cggagcggcc gaagggcacc ggcacgccgg cggcgcgcag
     1021 caggttcttg gtgagctcct tgtcctgggc gatggattca gccacggcgc tggtcaggtc
     1081 ggtctcggcg gcctggatgc ggcgctgctg gctgccccag ccgaactgca ccaggctgcc
     1141 ctcggtgagg cggcggaagg ggatgccccg cgccaccgca gcctgcacga tggaaccggt
     1201 ggacggcccc aggcgcacgt cctcgtccag ctcccgcagg cgcgccaacg cgtcggccag
     1261 atcgaaattg cgttcttcca gcgccgcggt gcacagatcc accgccagat cgaaggccag
     1321 gcggccgacc ttttcttcgg tgtactccac caccacctgg aacacaccct tctcgatggt
     1381 ggcggaggtg cggctgaagg tcaccggaca gccagcgctc gattgcaggc cgagggcggc
     1441 gagctcgagg gcgtgggcca gggagatggt gccgtgatgg ccttccggct tgaggaagcc
     1501 catctcggga aacaggttgc gcaggcgggc ttcatagccc ggcagtccgt cgaggtcgca
     1561 ttccccgtcg gcgcacgaca cgatggcttc gatggaggtg tgccggctcc acaggttggg
     1621 gccgcgcagg gcccggatgc gggaaacttc catggatgag agtccttgtc ttcgttctcg
     1681 cccggccgtc attgcggccg gatcgtcatg attcgggcag gtggaaggct cagtcgttgc
     1741 gcagcggcgc gttcgggtcg tagggcttga cgcccgcccg gatcagcgcc ggcgcgacgc
     1801 ccagcgccca ggccgccgcc accgccgcca gcagattgga gagcacaccc gggcgctcgc
     1861 cggcaccgtt gcgcagcatg gcgatcttgt tgagccgcgc caccgcttcg gccttgtcgc
     1921 cggtcgcgag gacgatggcg ccgtcgctca cgtacaccgt gcgcttgccc tcgctgcggg
     1981 cctgggcaat ggccggcgcc tggccgtcca cgccgtagaa gatcacctcg ccatcggaca
     2041 gctcggccat ctcgacgacc gccgggtcct cggcgttgag caccgcggcg ccctggggca
     2101 ggaccaggtc cacctgggtg cggaagacgt tccacacctt ctccggcgca tccatgtaga
     2161 actcgccgta atgcagcggc gcgtcgacgt tggtcaccac gcccaccagg cagcggtcat
     2221 acaccgtgcc gtcggacagg atgctgcggc agccgttctc gaagaccgcg acatccacgg
     2281 agcggttcat cagcacccgc tcggcggcgt cccagttggt gcgcgccggc gtctccacct
     2341 ggcggccgtc gaggaatacg ccttcgctgc tcgccagccc ggtgtactgg cccgacaggc
     2401 gcagcagatg ggtgacgagg cgcgccaccg gcgtcttgcc gcggctgccg gtgacgccca
     2461 ccacggggat gcgcgaatcc gcgtcgtgct cgaagaggtg ggccacgatg gcctcaccca
     2521 ccgggcgcgg ttcgccgacg ccgggcttga tgtgcatgag caggccgggg ccggcattga
     2581 cctcgacgat ggcacccttc tgcgcgccca ggggcaggga gatgtcctcg cagaccaggt
     2641 ccacgccggc gatgtcgagc ccgacgatgc gcgccgccag acaggccagc tcggcggtgt
     2701 cgggatgcac ctggtcggtg acgtcgaagg ccatgttgct ggtgcgcacg atcatcacat
     2761 cctggccgtc cggcgggatg gagctgccgg tgtagccctg ccgctccact tccatctggg
     2821 ccgccgggaa gcggtcgagg gtgatgcggt agaggggaaa ttcgtgctcg tcgccgcgac
     2881 gcgggtcgga attgatctgg aggtcgatga gctggtcgat ggtgtgcttg ccgtcgccca
     2941 ccacggacac cgtgtcgccc ttgttggccg ccaccatgcg cccgcccacc accagcaggc
     3001 gatgctcgct gccgcggacg aagcgttcga cgatgacctc ggagccctgg gccagggaca
     3061 gggggaaggc cttggccact tcgtccgggg tgctgatgtt ggtaaacacg ccgcgggcat
     3121 ggttgccgtc gctgggcttg accaccaccg ggtagccgat gtcgcccgcc gcctcgatgg
     3181 catcctccgg gctggtcacg atgcgccctt ccggcaccgg cacgccgcag gattccagca
     3241 ggctcttggt gaggtccttc tcgcgggaaa tgctctcggc gatggcgctg gtgcggtcgg
     3301 tctcggccgt ccagatgcga cgctggcggg cgccgtagcc cagctgcacc aggttgcctt
     3361 ccgacaggcg gatgtaggga atgcgccggt ccttgtcggc ggcggcctcg acgatgcagc
     3421 gggtgctggg gccgagcatg tacttgtcca ccagatcgcg cagacggcgc acggcggcgt
     3481 ccacgtcgta gggacgccct tcgatggccg ccatcagcag gtcgcggccc atgtgcaggg
     3541 cggccaggga cacatccttc tgccacgccc ggatgaccac cttgtagacg ccccggtcgg
     3601 aggtctcgcg ggccttgccg aagccgccgg acatgccggc caggttctgc agctcaaggg
     3661 tcacgtgctc caggatgtgg cccggccagg tgccatcccg caggcgcttg aggaagcccc
     3721 cccgctcgcc gatgctgcag cgatgctcga tcagggacgg cagcagggcc gtcagtcgct
     3781 cgtagaagcc gggaatcacg ttggaggggc agtcctccag gtcgtggatg tccacccagg
     3841 cttcgaggac ggcgcggtag gtccagatgt tcggcccccg caggtggaac acgttcagga
     3901 attcgatcgt tttgttggtc atggacaggg caggataagg tcgacccgct cttcggcggg
     3961 atcacggcag tgcatcggaa acccgatgtt cccttaacgc gaaaccttcg tggcggatta
     4021 ccggcggaaa aaataatcag tgtagagtgg gtgacgtgac cgcttccagc cgcccgctcc
     4081 gtccgccggg tatacgccag tgtaacgagt cagtaaactt cgcccgcaat tctgccctac
     4141 gccggccgat tgctgtggcc ggatttccgc gccccgtcct gcggccccct cgcgttccaa
     4201 gatgacctca cagcctgccc tcccgccccg cgcttcccac aacgccggca ccgacgccta
     4261 ttccgactac ctcggccccg ccgtacccgc cgactggcga cagcggctcg acccgaccct
     4321 ggaggcgggc gagaaggtgc tcgccagcgt cgtcctcgat ctggacaccg ccttgcgctt
     4381 ccgcggtggc ctgctggccg tcaccgaccg ccgcctgctg gcccgcgccc aggacgacga
     4441 cacctggcag gcctggacct accgcgacgg actccacctg cgtcaccggg atcacgccgg
     4501 cgtggccgcc ctcgaactga tggacgacgg cggccgcctg gcgggctggc gcttcaccct
     4561 ggcccaggac ccggccgccc gcaatctggc cgaccgcttc cacgcccagc tcgactgcgt
     4621 attgtcgggc cgtgccccca cgccgccgga gcccctcacc tgccccacct gcgacgcgcc
     4681 tctgccgccg ggcaccgacg aatgccccac ctgcgcgcgg gagatccaca ccccgccgtc
     4741 cacctggacc ctgttccgcc tgtggcgctt tgcccgaccc tacaagagcc agttgctgtc
     4801 cggcttcatc ctgaccctgc tggccaccgg cgccaccctg atcccgccct atctgaccat
     4861 gccgctgatg gacgagatcc tgatcccgtt ccagaacggc acacccatcg acacccaccg
     4921 ggtcacgctg atcctcggtg gcctgctggt ggcggccctc ttcgcctggg gcctgggctg
     4981 ggccaagacc tacatcctgg cgctggtgag cgagcgcatc ggcgccgacc tgcgcaccac
     5041 cacctacgaa cacctgcagg gcctgtcgct ggaatacttc ggtggcaagc gcaccggcga
     5101 cctgatggcg cgcatcggcg ccgaatccga ccggatctgc ctgttcctgt ccatgaacct
     5161 gctggacttc gccaccgacg tgctcatgat cgccatgacc gcggcgatcc tggtgtccat
     5221 caacccctgg ctggccctgg tcaccctggt gcccctgccc ttcatcgcct ggctcatcca
     5281 catcgtgcgc gacaagctgc gctacggctt cgagcaggtg gaccgggtct ggtccgaagt
     5341 caccagcgtg ctcgccgaca ccatccccgg catccgcgtg gtcaaggcct tcgcccagga
     5401 gaagcgtgag gtcgcgcgct tccgcgaggc caacgcccgc aacctggcgg tgaacgaccg
     5461 ggtcaacaag gtctggtccc tgttctcgcc caccgtcacc ctgctcaccg aggtgggcct
     5521 gctggtggtg tggggcttcg gcatctggct ggtgtccgag cgggacgtca ccgtcggtac
     5581 gctggccgcc ttcctggcct acatctcgcg cttctacacc cggctcgact ccatgagccg
     5641 catcgtctcc gtcacccaga agg
//
LOCUS       DAOQPA010000165         5631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8669_length_5631_cov_18.227582, whole genome shotgun sequence.
ACCESSION   DAOQPA010000165 DAOQPA010000000
VERSION     DAOQPA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8669_length_5631_cov_18.227582"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKX01_15800"
     CDS             complement(<1..857)
                     /locus_tag="PKX01_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HMZ77647.1"
                     /translation="MAVDDTAPRAPRQGPIALADLFDAALKDLTPNPNPRAPAPMAVP
                     TPAASGDAFLFSGNRHESVPRRLFVDRRLTPLERNAWQVFRLMLNDDGVTTFPTYEQL
                     RPWLASMPCAGQASHETVARALTLLRLTRWLSLVRRRRDPKTGRILGNLYVLHDEPLT
                     LFEAMQLDADYLALVSQSLGHSAKAVQVVGLNTLQEIADDPMLSGRTLPSRLQVLAER
                     LADQGITATASYPQEDAIHDSEEGPASLLRNGERPSSESEAGPKPAPDGALRNPKQDR
                     TVRSSRIDEV"
     gene            complement(861..1421)
                     /locus_tag="PKX01_15805"
     CDS             complement(861..1421)
                     /locus_tag="PKX01_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HMZ77648.1"
                     /translation="MSTSHPLNQAVIAQALYDLRNGQLRRCKAMGFGEAELDALKHPA
                     LISVLANASVSWCSVTVNREVLQRLLNQAQDVEKEIATVDRMLRLGASTEMVSRFYGL
                     THQEVALRREILGLPKRKGRHPVLDETQDTELWRQWKAVTSSRNVDLEDETSILDAAM
                     DLAEGMSLPLSVVWAAIRNWVDQGLD"
     gene            complement(1436..3109)
                     /locus_tag="PKX01_15810"
     CDS             complement(1436..3109)
                     /locus_tag="PKX01_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HMZ77649.1"
                     /translation="MADMTSQDMAGKLLAAGFERSGPAATALSDPIADTPMVVTLDQL
                     RPYDHDPRMKRNPAYEEIKASIRERGLDAAPAITRRPGEDHYIIRNGGNTRLAILREL
                     WSETKDERFFRISCLFRPWPERGEIVALTGHLAENELRGGLTFIERALGVEKAREFYE
                     QESSAALSQSELARRLAADGFPVQRSHITRMADAVRYLLPAIPTVLYGGLGRHQVERL
                     SVMRTACKRTWEHYAKDRTLPLDFDSFFQEVLAQFDTQGDEFAPQRVQDELIGQMSEL
                     LGIGYDVLALDLTESESRHRALVSDPTPPAAPPALPSPTAGTPPPPGVASSIAKPAAA
                     ASPPPASPPASEPTLREDTARETTTESPTAAPGDLLLEHIVSPAPTTERLQSIQRMVA
                     DQLGDALPPDFSASVLQSIPVQAGGLYPISDVWYIDAGLDTPDRLRIHIAQFAREIAG
                     EAALDECIEDRSDGIGFVCCAPAHAPSPLGRAVLALLITLTGQPTAEAGVDGAQLAAD
                     LPALLHGQGQHHGDCTRRLSDTALVKLFRLLRLARRLLDLEAGTTAPGT"
     gene            complement(3102..3371)
                     /locus_tag="PKX01_15815"
     CDS             complement(3102..3371)
                     /locus_tag="PKX01_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77650.1"
                     /translation="MANAHELARGHKRLRALIEFAVGEGWHVKRTPGGHLKFTKAGCA
                     AIYTSSTASDHRAELNARAQIRRAEREARMAPAGGRGDCEGCGHG"
     gene            complement(3355..4230)
                     /locus_tag="PKX01_15820"
     CDS             complement(3355..4230)
                     /locus_tag="PKX01_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ77651.1"
                     /translation="MQVVSIISTKGGVGKTTTAANLGGLAADAGLRVLLLDLDVQPTL
                     SSYYELTQRAPGGIYELLAFNERDLGQLVSRTIIAGLDLVLSNDHRGELNTLLLHAPD
                     GRLRLRHLLPALAPLYDLVLIDTQGARSVLLEMAVLASDLALSPVTPEILAARELRRG
                     TMQLLEDIAPYRHLGIEPPPLHLLINRVHPVSANARLIQQALRDLFQDHAGIRVLATD
                     VPAIEAYPRAATRGLPVHRVEHRQPPGRVAPASLDTMRALASELFPQWQDRFAAMSGR
                     PPRPLDAGRSHGERT"
     gene            complement(4273..4485)
                     /locus_tag="PKX01_15825"
     CDS             complement(4273..4485)
                     /locus_tag="PKX01_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HMZ77652.1"
                     /translation="MSQPPVLPPNERRILRLDEVEAKSGFKRAHIYNLMKKRQFPQAL
                     RLGVRAVGWDSIEIDQWIAERLNHRT"
     gene            complement(4605..5351)
                     /locus_tag="PKX01_15830"
     CDS             complement(4605..5351)
                     /locus_tag="PKX01_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77653.1"
                     /translation="MHKDIPTSKVFYRPIEAAIRWAGLLRYLPMILAAIASPRFLPPS
                     LNCPRWNECRLYSERIYDGILNGELPYGQNGITLNDPKLLQSPDLTVRHVDLKRWMRN
                     HYPEHRPGFLFSRGERMAHPFITLETGQAILIERLALQAALEHARRQMQELQEQRDTL
                     LKQSTALLASKQCEISERAETTYLNIIGGMLTLMLGQSPSGVPYSSFKTQEALVSALV
                     AHFGGTMGITERTLNGKFANARKYVRSASA"
BASE COUNT          888 a         1766 c         1972 g         1005 t
ORIGIN      
        1 acttcatcaa tacgactact acgtactgta cggtcctgct tcggattccg aagagcgccg
       61 tctggcgcgg gtttcggccc tgcttcggat tccgaagaag ggcgttcgcc attccgaaga
      121 aggctggcgg gcccttcttc ggaatcgtgg atcgcatcct cctgtggata acttgcggtg
      181 gcggtgatgc cctggtcggc caggcgctcg gcgagcactt gcagccgcga cggcaaggtg
      241 cgtccggaca acatcgggtc gtccgcgatc tcctggaggg tgttgagtcc caccacctgg
      301 acggccttgg cggaatgccc cagcgactgg ctgaccaggg ccaggtagtc ggcgtcgagc
      361 tgcatggcct cgaacagtgt caggggttcg tcgtgcagga cgtagaggtt tccgaggatg
      421 cggccggtct tggggtcgcg ccgtcgtcgc acgaggctca gccagcgcgt caggcgcagc
      481 agcgtcagcg cccgcgcgac ggtctcgtgg gaggcttgtc ccgcgcaggg catggacgcc
      541 agccatgggc gcaactgctc ataggtcggg aacgtcgtga cgccgtcgtc gttgagcatc
      601 agccggaaca cctgccaggc gttgcgctcc agcggtgtca ggcggcggtc gacgaacagc
      661 cgccgcggca cgctctcgtg ccgattgcca ctgaatagga aggcgtcgcc ggacgccgca
      721 ggcgtgggca ctgccatggg cgctggcgcg cggggatttg gattgggcgt gaggtccttc
      781 agcgcagcgt cgaacaggtc ggcgagtgcg atggggcctt gacgcggggc tcgtggggcg
      841 gtgtcgtcca cggccatggc ctagtccaat ccctgatcga cccagttcct gatcgcggcc
      901 cagaccaccg agagcggcag tgacatgccc tcggccaggt ccatggccgc atcgagaatc
      961 gaggtctcat cctccagatc gacgttcctg ctgctggtca cggctttcca ttgccgccaa
     1021 agttcggtgt cctgggtctc gtccaggacg gggtggcgcc ccttgcgctt gggcaggccg
     1081 aggatctccc gccgcagggc gacttcctga tgggtcaggc catagaagcg gctgaccatc
     1141 tccgtgctgg cccccagccg cagcatgcga tcgaccgtgg cgatttcctt ctccacgtcc
     1201 tgtgcctggt tgagcaggcg ctgcagcact tcgcggttga ccgtgaccga gcaccacgag
     1261 acgctggcgt tggccagcac gctgatcagc gcggggtgct tgagcgcatc cagctcggcc
     1321 tcgccgaagc ccatggcctt gcagcggcgc agttggccgt tgcgaaggtc atacagcgcc
     1381 tgggcaatca cggcctggtt gagcggatgc gatgtggaca tgcggcctcc ctcgctcacg
     1441 tcccgggcgc cgtggtgccg gcttccaggt ccagcagacg ccgggccagg cggagcagcc
     1501 gaaatagctt gaccaacgcc gtgtcgctca agcgccttgt gcagtcgccg tggtgttgac
     1561 cctggccgtg cagcagtgct ggcaagtcgg cggcgagctg cgcgccatcc acgcccgctt
     1621 cagcagtggg ctgacccgtc agagtgatga gcagcgccag caccgcccgg ccgagcggcg
     1681 atggtgcatg ggcgggcgcg cagcaaacga agccgatgcc atcggagcga tcctcgatgc
     1741 actcgtccag ggctgcctcc ccggcgatct cgcgcgcgaa ctgcgcgatg tggatgcgca
     1801 gccgatcggg cgtgtccagg ccggcgtcga tgtaccagac gtcggagatc ggatagagcc
     1861 cgccggcttg caccgggatg gactgcagca cgctcgccga gaagtcgggc ggcagcgcat
     1921 cgcccaactg gtcggcaacc atgcgctgga tcgactgcag ccgttcggtc gtcggtgccg
     1981 gcgagacgat gtgctcgaga agcagatcgc ctggtgcggc cgtggggctt tccgttgtcg
     2041 tctctcgggc cgtatcctcg cgcaaggtgg gctcggacgc cggagggctg gcgggtggag
     2101 gactggccgc cgctgcaggc ttggcgatgg acgatgctac gcccggggga ggcggcgtgc
     2161 cggcggtggg cgaaggcaac gccggcggcg ccgcaggtgg cgtcgggtca ctgaccagcg
     2221 cccggtggcg gctttccgat tcggtcaggt ccagcgccaa cacgtcgtag ccgatgccca
     2281 gcagctcgga catctggccg atcagctcgt cctgcacgcg ctgcggcgcg aactcgtcgc
     2341 cctgcgtgtc gaactgcgcc agcacctcct gaaagaagct gtcgaagtcc agcggcagcg
     2401 tgcggtcctt ggcatagtgc tcccaggtgc gcttgcaggc cgtccgcatg accgacagcc
     2461 gctcgacctg gtggcgcccc aggccgccat agagcacagt gggaatggcg ggcagcaggt
     2521 agcgcaccgc gtcggccatg cgggtgatgt gcgaccgctg gacggggaac ccatcggccg
     2581 ccaggcggcg ggccagttcc gactggctca gggcggcgct actctcctgc tcgtagaact
     2641 cgcgtgcttt ctcgacgccg agcgcgcgct cgatgaacgt gaggccaccg cgcagttcgt
     2701 tctcggcaag atgcccggtc agcgcgacga tttcaccgcg ctccggccat ggccgaaaca
     2761 ggcacgatat gcggaagaag cgttcgtcct tggtctccga ccagagttcg cgcaggatcg
     2821 ccagccgcgt gttgccgccg ttgcggatga tgtagtggtc ctcgccgggc cggcgggtga
     2881 tggctggcgc cgcgtccagg ccgcgctcgc ggatggacgc cttgatttcc tcgtaggcgg
     2941 ggttgcgctt catgcgcggg tcatggtcgt agggccgcag ctggtccagc gtcacgacca
     3001 tgggcgtgtc cgcgatcggg tcgctcaagg ccgtagccgc tgggccgctg cgctcgaagc
     3061 cggccgcgag cagcttgccg gccatgtcct gcgaggtcat gtcagccatg gccgcacccc
     3121 tcgcagtccc cacgaccgcc ggccggcgcc atgcgggcct ctcgctcggc gcggcggatc
     3181 tgcgcacggg cgttcaattc ggcccggtgg tcgctcgcgg tcgaactggt gtagatcgcc
     3241 gcgcagccgg ccttggtgaa cttgaggtgc ccgcccggcg tgcgcttgac gtgccagcct
     3301 tcaccgaccg cgaactcgat cagcgcgcgc agccgcttgt ggccgcgggc cagttcatgt
     3361 gcgttcgcca tgggacctcc cggcatcaag aggacgtggc gggcgaccgg acattgcggc
     3421 aaatctgtcc tgccattgcg ggaacagctc gcttgcgagc gcgcgcatcg tgtcgagcga
     3481 ggccggcgcg accctgccgg gcggctggcg gtgctcgacc cgatgcaccg gcaggccgcg
     3541 cgtcgcggcg cgcggatagg cttcgatggc tggcacgtcg gtggcgagca cgcggatgcc
     3601 ggcatggtct tggaacaggt cgcgcagggc ctgctggatc agtcgggcgt tcgccgacac
     3661 cgggtggacg cggttgatga gcaggtgcag cggcggcggt tcgatgccca ggtgccggta
     3721 cggcgcgatg tcttcgagca actgcatggt gccgcgtcga agctcccggg ccgcgaggat
     3781 ttccggcgtg acgggcgaca gcgcaaggtc ggaggcgagc accgccatct ccagcagcac
     3841 ggaacgcgcg ccctgggtgt cgatcagcac caggtcgtag aggggagcaa gtgctggcag
     3901 cagatgccgc agccgcaggc ggccatccgg cgcatgcagc aataaggtgt tcagctcgcc
     3961 ccggtggtcg ttggacagca ccaggtccag gcccgcgatg atcgtgcggg acacgagctg
     4021 gccaaggtcg cgctcgttga aggccaacag ctcatagatg ccgcccggcg cgcgctgggt
     4081 cagttcgtag taggaggaca aggtgggctg cacgtcgaga tcgagcagca gcacgcgcag
     4141 gcccgcgtcc gcggcgagcc cgcccaggtt ggcggccgtc gtggtcttgc cgacgccgcc
     4201 cttcgttgaa atgatggata cgacctgcat ggcgctctcc ggctgggaat gggaagtccg
     4261 cgggagaacg ggtcaggtcc ggtggttgag gcgctcggcg atccactggt cgatttcgat
     4321 cgaatcccag ccgacggcgc gcacgcccag gcgcagcgcc tggggaaact ggcgcttctt
     4381 catcaggttg taaatgtggg cgcgtttgaa gccggacttg gcttcgactt catcgagccg
     4441 caggatgcgg cgctcgttcg gcgggagtac agggggctgc gacatggcgg tcactcctga
     4501 acgctcggtg gcgtttgttg gcgtgacctc tattcaatag acctggctgc ggaaagacat
     4561 tgcaaatgca atctccgcgg ttgcacatac aagctggaaa aacctcatgc gcttgcgcta
     4621 cggacgtact tcctggcgtt ggcgaacttg ccgttcaagg ttcgctccgt gatgcccatg
     4681 gtgccgccga agtgggcgac caatgccgag acgagtgcct cctgtgtttt gaagctggaa
     4741 tagggcacac ccgaagggga ctggcccagc atcagcgtca gcatcccgcc gatgatgttg
     4801 agatacgtcg tctccgctcg ctcgctgatc tcgcattgct tggaagccag cagcgcggtg
     4861 gactgcttga gcaaggtgtc gcgctgctcc tgcaattcct gcatctggcg acgcgcatgc
     4921 tccagggccg cctgaagggc cagtcgctcg atcaggatcg cctgtcctgt ttccaaggtg
     4981 ataaaaggat gcgccatgcg ttcgccacgg ctgaacagga atcctggccg gtgctcggga
     5041 tagtggttgc gcatccagcg cttcagatcg acatggcgaa cggtcagatc cggcgactgc
     5101 agcagcttcg gatcattgag cgtgatgccg ttctggccgt agggcagctc cccgttgagg
     5161 atgccgtcat agatacgctc cgagtacagc cggcactcat tccatcgcgg gcaattcaac
     5221 gatggaggca ggaagcgcgg tgacgcgatc gcagccagga tcatcggcag gtaccgcagc
     5281 aacccggccc accgtatggc agcctcgatg ggacgataga acaccttcga tgttggaatg
     5341 tccttgtgca tgtcttcgtc tcctttcgga agcgctacat caccggctcc tttcttgccc
     5401 tgccaagggc tgctgtgtcg ttatgacctt ccgcgggtgc ctgggaagat gccctgaacg
     5461 tagacgggcg aggtgattgg tttggcgtcc gctgtaggtg gcctcgtctt gctcgttgtg
     5521 caagaatcga tcggttgccg gcccatcgag cgatgaggac gaaagctcga tattggcgct
     5581 cacggtacca gcgtcatgcg ctgcgctgtt ccccgcaaaa ccgatttggt a
//
LOCUS       DAOQPA010000166         5628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8675_length_5628_cov_18.390992, whole genome shotgun sequence.
ACCESSION   DAOQPA010000166 DAOQPA010000000
VERSION     DAOQPA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8675_length_5628_cov_18.390992"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5628)
                     /locus_tag="PKX01_15835"
     CDS             complement(<1..>5628)
                     /locus_tag="PKX01_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HMZ77654.1"
                     /translation="NTLDTTAGTLQHAGAGTFSLSADTFTAPGATLQSNGTLDLLAQR
                     LSLDHATTTAQTLRLSARSFSHQAGTLLQTGAGTASIQADVLDNAGGQIVSNGGLDLV
                     AGALNNRGASIQAATGALTLNAGDVDNTGGLIAAQQNTTIGTAVLNNSGGDLRSGGKL
                     TLSATGTVTNAGGRIASAADLTLSASTLDNTSGRMGAIGGNLTIETTSSPLNNASGHL
                     EAAGLARVSVKGFNNQGGLLTASSIELDTDGHALDNTSGRLIASGRGDVDRFDIRSGP
                     LDNRGGLIQAGRRLRVDTGAYGLSNVATQISGGLVSQGTLDILSAAVDNGAGFIGAQG
                     AMTLLAGMFANTQGGQVIGGDRIALSATTLDNRGGIVQGVGDVTLHLADLLDNRNSLI
                     RAGGTLAFSADRLLNSDTQNAALGLEGSAIRGEVRLIDNTGGAIRATGDLSLSSHGHI
                     LNIGGSMSAGGTLSLHDPAAVRSLTVTQTGGTLLAGRLLDIDADSLGGDGELLSNGDL
                     RIRVQQDYTHTGHLQAVGDARLETAGVLVNRAAPVAGGTLSLQGASVDNQAGAILNAG
                     RLELRATDSHTFTNRGLIDSSETFIDAITLNNLGTGRIYGDHLAITAVTLNNLAEGGT
                     APVLAARDRLDLGVGTLTNAEGALIFSAGDLALGGALDASRRATGQGTALVNSSATVE
                     ALGNMDLRVARLDNLNAHYTTVLETVSQTHVEEYQGKDAIKRYLPGSPDVYIYNDESD
                     YLHTPDGNYEEWSIYSYDRTVREERLLTSAPARIVAGGRITLTSDRVLNDRSQILAGG
                     LLDGTIGTLSNVSGIGQRIFSDIGTVTNKWREHPSGRDYTGSATHDYAPPDTVEGIDL
                     VPAAFQSLAATTETGLSLDPAKVQVSNSSIQTAGTVRPGSLTQADVIRDTAIDPLSAR
                     AADRADAIAAGPDAIVPSGRPPAATPGLSVTSAPSSALSPVSGAPGGDHAAALSIPTA
                     ASAQIPPPAGGEATPPSVVASPTGDHGTFPALPAGRPIDGLTGATANPIASPSAERWT
                     PGPQDIPTAHAEVSTRSAQARPADPLPAPSATHSPAPRPRSVTPILQVAATTASGTAS
                     VVRSGGVDVAIPSNSLFRAHPEVNGRYLVETDPAFTRYEAWLGSDYLLQALAMDPATT
                     QKRLGDGFYEQKLIRDQVAQLTGRRFLDGYASDEAEYRALMDAGLTVAKDWNLRPGVA
                     LSAAQMALLTRDMVWLVEQSVQLPDGSIQKVLVPQVYVRVRDGDLSASGALVAGRDVQ
                     LDVRGELMNQGTISAQGTARLSADTLRNLNGHIVGGDLTLQATGDLLNRGGELRGDTR
                     LSVAAGRDLIVESTTRSQDNDQGSRTHIDHLASLSVTGPNGALTALAGRDLTLSGATV
                     QSAGSVTLAAGNTVNLGTVGESSAHRIVWDANNWRTDRSTREVGTTVGAQGKVQMLAG
                     QDIALRAATVTSQNGAIALSAGRDVQILAGQSLDQVDAAHQHTSRGFLSSKTITTRDQ
                     VNLLGVQGSTLSANTVTVEAGRNVQITGGQLVSDQGTRIAAAGDLNIDAATETRGERH
                     LAATRSSGLLRSGLGVTLGSRELSVDGLTDSTRAVASVVGSTAGSLQIQAGKALRLTG
                     SDLQAPSGDIALQAPRIDIAEARETLHTQTETRFRQSGITVAVSTPALTALQTAQQMN
                     RAASQTADPRMKALAAASTALSAYSAGQAIVAGQGVTLPDGRTGQIVTGKDATGEPIS
                     RDATTADRIGGINITVSAGSSRSVSTQVVDSDTGRSSLVQAGRGVAITARGGANDGTL
                     NVRGSTLSAGTTLTLDADNTLKLLAAQNRTTQTSANQSTSGSLGVTAGSGGVGVTLSA
                     SSGRGHSDGTDLSQLNTHLQAGQDIDLRSGGDTT"
BASE COUNT          702 a         1722 c         2210 g          994 t
ORIGIN      
        1 cgtggtgtcg ccaccgctgc gcaggtcgat gtcttgcccg gcctggaggt gggtgttgag
       61 ctggctgagg tcggttccgt cgctatggcc gcggccgctt gacgcgctga gggtgacgcc
      121 cacgccgccg ctgcccgctg tgacgccgag cgagccgctg gtggactggt tcgcggaggt
      181 ctgtgtcgtc cggttctggg cggcgaggag cttgagggtg ttgtccgcat ccagtgtcag
      241 ggtggtgccg gcggagaggg tgctgccgcg gacgttcagg gtgccgtcgt tggcgccgcc
      301 gcgcgccgtg atcgccacgc cgcggcccgc ctgcacgaga ctgctccgtc cggtgtcgct
      361 gtcgacgacc tgggtgctga cgctgcggct gctgccggcg ctgacggtga tgttgatgcc
      421 ccctatccgg tcggcggtgg tggcgtcccg gctgatgggc tcacctgtgg cgtctttgcc
      481 agtcacgatc tgaccggtgc ggccgtcggg cagggtgacg ccctgaccag cgacgatggc
      541 ctgtcccgcg gaataggccg agagggcggt gctcgcggcg gccagggcct tcatgcgagg
      601 atctgcggtc tggctggccg cgcggttcat ctgctgggcg gtctgcaggg ctgtcagtgc
      661 cggagtcgac acggcgacgg tgatgccgct ctggcggaaa cgggtttcgg tctgggtgtg
      721 gagcgtctcg cgtgcctcgg cgatgtcgat gcggggcgcc tgaagggcga tgtcgccgga
      781 gggcgcctgg aggtcgctac ccgtgaggcg caaggcctta ccggcctgga tctgtaggct
      841 gccggcggtg ctgccgacca cgctggccac tgcgcgggtg ctgtcggtga ggccgtctac
      901 actgagttcg cggctgccga gggtgacgcc cagaccgctg cgcagcaaac cgctgctgcg
      961 ggtggcggcc aggtgtcgtt cgccgcgggt ttcggtggcg gcatcgatgt tcaggtcgcc
     1021 ggctgcggcg atgcgggtgc cctggtcgct gacgagctgg ccgccggtga tctggacatt
     1081 gcggcccgct tcgacggtga cggtgttggc cgacagggtg ctgccctgca cgccgagcag
     1141 attgacctgg tcgcgggtcg tgatggtctt ggacgaaagg aagccgcggg aggtgtgctg
     1201 gtgggcggcg tccacctggt cgagggactg gccggcgagg atctgcacgt cgcgcccggc
     1261 ggacagcgca atggccccgt tttggctggt gacggtggcg gcgcgcaggg cgatgtcctg
     1321 ccccgcgagc atctgcacct tgccctgagc gccgacggtg gtgccgacct cccgggtgct
     1381 gcggtcggtg cgccagttgt tggcgtccca cacgatgcga tgggcgctgc tctcgccgac
     1441 ggtgcccagg tttacggtgt tgccagcggc aagggtcaca ctgccggcgc tctgcacggt
     1501 cgcgccgctc agggtgagat cgcggccggc cagggcggtc agcgcgccgt tcgggccggt
     1561 aaccgacagg ctggcgagat gatcgatgtg ggtccggctc ccctggtcgt tgtcctggct
     1621 gcgggtcgtg ctttcgacaa tcaggtcgcg gcctgcggcc actgagaggc gcgtatcgcc
     1681 ccggagctcg ccaccccggt tgagcagatc gcccgttgcc tgcagcgtga ggtcgccgcc
     1741 gacgatgtgg ccattgaggt tgcgcagggt gtccgccgag aggcgggccg tgccctgggc
     1801 agagatcgtg ccctgattca tcaactcgcc ccggacgtcg agctggacat cccgaccggc
     1861 cacgagggcg ccactggcgc tgaggtcgcc gtcccgcacg cgcacataga cctggggcac
     1921 gagcaccttc tggatcgagc cgtcgggcaa ctggacggac tgctcgacga gccagaccat
     1981 gtcgcgggtg agcagggcca tctgcgcggc gctcagggcg acgcccgggc gcaggttcca
     2041 gtccttggcg accgtgaggc cggcgtccat cagggcacgg tactccgcct cgtcgctggc
     2101 gtagccgtcg aggaagcgcc ggccggtgag ctgcgccacc tgatcgcgga tcagcttctg
     2161 ctcgtagaag ccgtcaccga gtcgcttctg ggttgtggcc gggtccattg ccagcgcctg
     2221 cagcaggtag tcggaaccga gccaggcctc gtagcgggtg aaggccgggt ctgtttccac
     2281 gagatagcgg ccattcacct ccgggtgggc gcggaacagg ctgttgctcg ggatggccac
     2341 gtcgacgccg ccgctgcgga ctacgctggc ggtgccgctg gcagtggtgg ccgcaacctg
     2401 caggatgggg gtgaccgacc gggggcgggg ggccggggag tgggttgcag acggcgccgg
     2461 gagaggatcg gcggggcggg cctgtgcgga ccgggtcgag acctctgcgt gggcggtggg
     2521 gatgtcctgg gggccgggcg tccaccgttc ggctgacgga ctggcaatcg ggttcgctgt
     2581 cgcaccggtg agcccgtcga tggggcgacc cgccggcagg gcagggaagg tgccgtgatc
     2641 gccggttggg gaggccacga ccgatggggg cgtggcctcg ccgccagcag ggggcgggat
     2701 ctgggcggac gctgccgtcg ggatggagag ggccgccgca tgatcgcccc ctggcgcccc
     2761 cgataccggc gagagcgcgc ttgacggggc ggacgtgacc gaaaggccag gcgtcgcggc
     2821 agggggacgt cccgacggga cgatggcatc cggaccggcc gcgatggcgt ccgcgcgatc
     2881 cgccgcgcgg gcactgaggg ggtcgatggc ggtgtcacgg atgacatccg cctgcgtgag
     2941 gctgccgggg cgcacggtcc cggccgtctg gatcgacgag ttcgaaacct gcaccttggc
     3001 gggatcgagc gacaggcctg tctccgttgt ggccgcgagg ctctggaagg cggcggggac
     3061 gaggtcgatg ccctcaacgg tgtcgggcgg tgcgtagtcg tgggtggcgc tgccggtgta
     3121 gtcgcggccg ctaggatgtt cccgccactt gttggtgacc gtaccgatgt cggagaagat
     3181 gcgctgaccg atgccgctga cgttgctcag ggtgccgatc gtcccgtcga ggagtccgcc
     3241 ggcgaggatc tggctgcggt cgttgaggac gcggtcggac gtcagggtga tgcggccgcc
     3301 ggcaacaatg cgcgccgggg cgctggtgag cagccgttct tcccggacgg tccggtcata
     3361 gctgtagatg ctccattctt cgtagttgcc gtccggcgtg tgcaggtagt cagactcgtc
     3421 gttgtagatg tagacgtccg ggctgcccgg cagatagcgc ttgatggcgt ccttgccctg
     3481 gtattcctcc acgtgggtct ggctcacggt ttccaggacg gtcgtgtaat gggcgttgag
     3541 gttgtcgagg cgggccacgc gcagatccat gttgcccagc gcttcgacgg tggcgctgga
     3601 attgacgagg gccgtgcctt ggccggtggc gcggcggctg gcatccaggg cgccgccgag
     3661 ggccagatcg ccagcgctga agatgagggc gccttccgcg ttggtgaggg tgcccacgcc
     3721 cagatcgaga cggtcccggg cggccagcac tggcgcggtg ccgccttcgg ccagattgtt
     3781 cagcgtgacg gccgtgatcg cgaggtggtc gccatagatc cggcccgtgc ccaggttgtt
     3841 gagggtgatc gcgtcgatga aggtctcgct gctgtcgatc agcccgcggt tggtgaaggt
     3901 gtggctgtcg gtggcgcgca gttcgagccg gccagcgttc aggatggcgc ccgcctggtt
     3961 gtcgacgctg gccccctgca ggctgagggt gcctccggct accggggcgg cgcgattgac
     4021 gagcacgccg gcggtctcga ggcgggcgtc gccaacggcc tggaggtgcc cggtgtgggt
     4081 gtagtcctgc tggacgcgga tgcgcagatc gccgttggac agcagttcgc cgtcgccgcc
     4141 caggctgtcg gcgtcgatgt ccagcaggcg gccggcaagc agggtcccgc ccgtctgggt
     4201 gacggtcagg ctgcggacgg cggcgggatc gtgcaggctg agcgtgccgc ctgccgacat
     4261 gctgccgccg atgttgagaa tgtggccgtg actgctgagg ctcagatcgc cggtcgcgcg
     4321 gatggcgccc cccgtattgt cgatgaggcg gacttccccc ctgatggcgc tgccctccag
     4381 accgagcgcg gcgttctgtg tgtccgagtt gaggagccgg tcggcggaga aggcgagcgt
     4441 tcccccggcg cggatcaggc tgttccggtt gtccagaagg tcggcgaggt gcagcgtgac
     4501 gtcgccgacg ccctggacga tgccgccccg gttgtccaga gttgtggcgg acagcgcgat
     4561 gcggtcgccc ccgatgacct gtccgccttg cgtgttggca aacattcctg caaggagtgt
     4621 catcgcgccc tgggcaccga tgaaaccggc cccgttgtcc acggcagccg agaggatgtc
     4681 gagcgtcccc tggctgacca gaccgccgct gatctgtgtg gcgacattgc tgagcccgta
     4741 cgcgccggtg tccacgcgca ggcggcgacc cgcctggatc agtccgccac ggttgtccag
     4801 ggggccgctg cggatgtcga atcggtcgac gtcgccgcgt ccggaggcga tcaagcgacc
     4861 ggatgtgttg tcgagcgcgt gcccgtctgt gtccagttcg atgctggagg cggtgagcag
     4921 gccgccctga ttgttgaagc ccttcacgct cacgcgggca agtcccgcgg cttccaggtg
     4981 gccggacgcg ttgttcagcg gactgctcgt cgtttcgatc gtcagatttc cgccgatggc
     5041 ccccatgcgc ccggacgtgt tgtcgagcgt cgatgcggac aaggtcaggt ccgcggctga
     5101 agcaatgcgg ccgccggcgt tggtgaccgt gccggtggcg ctgagcgtga gcttgccgcc
     5161 gctgcggagg tcgccgcccg agttgttcag cacggcggtg ccgatcgtgg tgttctgctg
     5221 ggcggcaatg agtccacccg tgttgtccac gtcacccgca ttcagcgtga gcgcccccgt
     5281 tgcagcctgg atggaagcgc cgcgattgtt cagggcgcct gcgaccagat cgaggccacc
     5341 gttgctgacg atctgcccgc cagcgttgtc gaggacgtct gcctggatcg aggcggtgcc
     5401 ggcaccggtc tggagcaggg tgccggcctg gtggctgaag gaccgggcgc tgaggcggag
     5461 cgtctgggcg gtggtggtgg cgtggtcgag gctcaggcgc tgggcgagca ggtcgagggt
     5521 gccgttgctc tggagcgtcg cgccgggggc ggtgaacgtg tcggccgaga ggctgaaggt
     5581 cccggcgcct gcgtgctgca aagtgccggc agtggtgtcg agcgtatt
//
LOCUS       DAOQPA010000167         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8754_length_5589_cov_16.027467, whole genome shotgun sequence.
ACCESSION   DAOQPA010000167 DAOQPA010000000
VERSION     DAOQPA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8754_length_5589_cov_16.027467"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..707
                     /locus_tag="PKX01_15840"
     CDS             363..707
                     /locus_tag="PKX01_15840"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMZ77655.1"
                     /translation="MIAPLRVAVATKNRVQIDEHFGHADEFKIYAVGPDGIAFEEVRV
                     VEHYCQGGFGDEDKREVILRALADCTALFCAKVGDGPAKRLREAGIEPVGDYPYGAIE
                     DSLRAWFAGRPA"
     gene            complement(712..1731)
                     /locus_tag="PKX01_15845"
     CDS             complement(712..1731)
                     /locus_tag="PKX01_15845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ77656.1"
                     /translation="MNAVSVSSPLRSLGPLRVPVAPIGLGCMGMSEFYGPSDDAESLA
                     TLARALELGVTLFDTADTYGHGANERLLGRFIAEGGAARRARMVIATKFGIVREPGRY
                     ERRIDNSPAYLRAACEASLQRLGVEAIDLYFCHRRDPAVPVEDMMGALAALVQAGKVR
                     QVGLSEVSVDTLRRAHAVHPVAAVQSEYSLWSREPEQGMLAACAELGTRFVAYAPLGR
                     SFLTGTTDVAALADNDFRKHNPRFAAEAVERNRQLVEALARFAEARGASSAQIALAWL
                     LAKHPHVIPIPGTRRIRWLEQNMAAARIALDADEVAQLDALFAPEQVVGARYPEAGWV
                     GVERG"
     gene            complement(1824..2597)
                     /locus_tag="PKX01_15850"
     CDS             complement(1824..2597)
                     /locus_tag="PKX01_15850"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ77657.1"
                     /translation="MQTSTQFPDLAGKVILVTGASSGIGAATALALGRAGARVALAAR
                     RLDSLEALAAQIRDAGGEALTVQTDVTVESQVRDAVAATVARFGRLDGAFNNAGALGA
                     AAPLHESTTDNFDAVFKANVYSVYWSMKYEIAALLQTGGGAIVNTSSIVAQIGFPGVA
                     AYTASKHAVMGLTRNAALEYFTQGLRINAVLPGPIETPMAESGFGSLEALRESMKASP
                     AGRAGVPDEVAAPVLFLLSDGARYMSGQALTVDGGYTVA"
     gene            2703..3617
                     /locus_tag="PKX01_15855"
     CDS             2703..3617
                     /locus_tag="PKX01_15855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ77658.1"
                     /translation="MNAPPLASLNDLHTFATVARTRNFRRAAAELGVSPSALSHALRA
                     LEARLGVRLLNRTTRSVAPTEAGQRLLDRVAPALRELHGALDAVNAFRDSPLGTLRIN
                     APRAACAWVLAPLVSRFLAEYPGMRVELVADDRFVDIVAGGFDAGVRFGESVQQDMVA
                     VPLGAAQHFVVVAAPAYLAARGTPDCPRALTAHSCIRVRFPGGQYYRWEFRRGSEQVE
                     IEVDGPLATDDMHVMIAAAEAGLGLAYVYAQYAAPAIAAGRLVSVLDDWRPPEPGFFL
                     YYPSHRLPPAGLRAFIDLARRIHPDNRP"
     gene            3679..4164
                     /locus_tag="PKX01_15860"
     CDS             3679..4164
                     /locus_tag="PKX01_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HMZ77659.1"
                     /translation="MRPLPRLACLALLLAALLAASAYADELRHVSLPDARYADAREAL
                     VDAIEGEGLIPSPPSRFGDMLARTGPALGQDGAVYSDAEVLHFCSARVAWQLARENPV
                     NIAQCPLSMAIYTRAGEAGTVHLAWRPTQGDSAGSRAANALLERIAAQVSDNTGRARP
                     R"
     gene            4268..>5589
                     /locus_tag="PKX01_15865"
     CDS             4268..>5589
                     /locus_tag="PKX01_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77660.1"
                     /translation="MTQKHYTADSITVLKGLEPVKQRPGMYTRTEHPLHVIQEVIDNA
                     ADEAMAGFCRRIGVTLHADGSVSVADDGRGIPVEIHPVERVSTVEVVFTRLHAGGKFN
                     KTDEGSAYRFSGGLHGVGVSVTNALARRLEVEVVRDGGRHRLVFAGGDVIEPLVRIGD
                     APKKASGTTVRVWPDAKYFDAAELPRGELERLLRSKAVLMPGLDVSLAVEGGDTRNWR
                     FDDGMRGYLNESLTGEPLIPLFSGEKYAPKGDETFAAGEGAAWAVAWTAEGAPVRESY
                     VNLIPTPAGGTHEAGLRDGIFTAVKNFIDHHALLPKGVKLTADDAFGRASFVLSARVL
                     DPSFQGQTKERLNSRDAVQLVSRMVRDPFELWLNEHVEYGKKLAELAIKAAQARARAA
                     QKVEKRKSSGVAVLPGKLSDCESEDIARNELFLVEGDSAGGSAKMARD"
BASE COUNT          884 a         2171 c         1750 g          784 t
ORIGIN      
        1 cgatgatcgc cgaggaactg gccaaggtga aggccaccgt caccgcccag ggcgaagaca
       61 ccgccaccta cgaccaggcc gcggtcatct tcgacaagat gtccctgacc ccggactacc
      121 cggaattcct gaccctgccg ctgtacgagg cgatggaata aagcgccccc cgaaacgatc
      181 cgacggcgaa cccgccggat cgtgacggac ccacgggttg agaaacggca tcctcgaggg
      241 gatgccgttt tgacgtcaac acgtcgacga acccatctcg aacgattttg gcaccgcctt
      301 tgcttgatgc ctcccgacct tgtcgcacct gcgacacccc acgactatcg ggagtccatg
      361 ccatgatcgc cccccttcgc gtcgccgtgg cgacgaaaaa ccgtgtccag atcgacgaac
      421 atttcggtca tgccgacgaa ttcaagatct acgccgtcgg tcccgacggt attgccttcg
      481 aggaagtgcg ggtcgtcgag cactattgcc agggcggctt cggcgacgag gacaagcggg
      541 aggtgatcct gcgcgccctc gccgactgta cggccctgtt ctgcgccaag gtgggcgacg
      601 ggcccgccaa gcggctgcgg gaggctggca tcgagccggt gggggactat ccctacggcg
      661 ccatcgaaga ttccttgcga gcgtggttcg cgggccgccc ggcctgacgc gtcagccccg
      721 ctccacgccc acccagcccg cctccgggta gcgcgcgccg accacctgct ccggcgcgaa
      781 cagcgcgtcc agctgcgcca cctcgtccgc atcgagggcg atgcgggccg ccgccatgtt
      841 ctgttccagc cagcggatgc gccgcgtgcc ggggatcggg atcacgtggg gatgcttggc
      901 cagcagccag gccagggcga tctgggcact gctcgcgccg cgcgcctcgg caaagcgggc
      961 cagggcctcc accagttgcc ggttgcgctc gacggcctcg gcggcgaagc gcgggttgtg
     1021 cttgcggaaa tcgttgtccg ccagggccgc cacgtcggtc gtgccggtga ggaaggaccg
     1081 cccgaggggc gcataggcga cgaatctcgt ccccagctcg gcgcaggcgg cgagcatgcc
     1141 ctgctccggc tcgcggctcc acagggaata ctcgctctgc actgccgcca cgggatgcac
     1201 cgcgtgggcg cgccgcaggg tgtccaccga cacctccgac agacccacct gacgcacctt
     1261 gccggcctgc accagcgctg ccagcgcgcc catcatgtcc tccacgggca cggccggatc
     1321 gcgccggtgg cagaaataca ggtcgatcgc ctccacgccc aggcgctgca gcgaagcctc
     1381 gcacgcggca cggaggtagg ccggcgaatt gtcgatgcgc cgctcgtagc ggccgggctc
     1441 gcggacgatg ccgaacttgg tggcgatcac catgcgcgcc cgccgtgcgg cgccgccctc
     1501 ggcgatgaag cgcccgagca gacgttcatt ggcgccatgg ccgtaggtgt cggccgtatc
     1561 gaacagggtc acgcccagtt ccagggcccg cgccagggtc gccagcgact ccgcgtcatc
     1621 ggacgggccg tagaactcgc tcatgcccat gcagcccagg ccgatgggcg caacgggcac
     1681 ccgcagcggc ccgaggctgc ggaggggtga ggaaacggac acagcgttca tgcaggcact
     1741 cctggtttca aagcattgcg aggccgggcc ggcgggccgc caccatcgcg ccccgccggt
     1801 ccatcacgat acggtaagca gcctcacgcc accgtgtaac cgccgtccac ggtcagcgcc
     1861 tgaccgctca tgtaacgggc cccgtcggac agcaggaaca gcaccggcgc ggccacctcg
     1921 tcgggcacac cagcgcggcc ggcgggcgac gccttcatgc tctcccgcag cgcctccagg
     1981 ctgccgaagc cggattccgc catgggcgtc tcgatggggc cgggcagcac cgcgttgatg
     2041 cgcaagccct gggtgaagta ttccagcgcc gcgttgcggg tcaggcccat caccgcgtgc
     2101 ttgctggccg tgtacgccgc cacgccggga aagccgatct gcgccacgat ggacgaggta
     2161 ttgacgatgg ccccgccgcc cgtctgcagc agcgcggcga tctcgtattt catcgaccaa
     2221 tacacgctgt acacattggc cttgaacacc gcgtcgaagt tgtccgtcgt gctctcgtgc
     2281 aggggcgcgg cggcccccag ggcacccgcg ttgttgaagg cgccgtccag gcggccgaag
     2341 cgcgccaccg tcgccgccac cgcatcgcgt acctggcttt ccacggtcac atcggtctgc
     2401 accgtcagcg cctcgccacc ggcatcgcgg atctgcgcgg cgagggcttc caggctgtcc
     2461 agccggcgtg ccgccagcgc cacccgcgcg ccggcgcggc ccagcgccag ggccgtggcg
     2521 gcgccgatgc cggacgaggc gccggtgacg aggatgacct tgccggccag atcgggaaac
     2581 tgtgtgcttg tctgcattgc gggctcctgt cgaagattgg ggatgaccgg atcagcgtaa
     2641 cgggccgcgc tccatgcaac aatccgcaca ctgacgcatg acttattgag cacatctcaa
     2701 caatgaacgc tccgcccctg gccagcctca acgatctgca cacctttgcc accgttgccc
     2761 ggacccgcaa cttccgccgc gccgcggccg agctgggcgt ctccccgtcg gccctgagtc
     2821 acgccctgcg cgcgctggaa gcccgcctcg gcgtgcgcct gctcaaccgc accacccgca
     2881 gcgtcgcgcc caccgaagcc ggtcagcgcc tcctcgaccg ggtcgctccg gccctgcggg
     2941 agcttcacgg cgccctcgac gccgtcaatg ccttccgcga ttcgcccctc ggcaccctgc
     3001 gcatcaacgc gccccgcgcc gcctgcgcgt gggtgctggc gcccctggtc agccgcttcc
     3061 tcgccgaata ccccggcatg cgcgtggaac tcgtcgccga cgaccgcttc gtggacatcg
     3121 tcgctggcgg tttcgatgcc ggcgtgcgct tcggcgagag cgtgcagcag gacatggtcg
     3181 ccgtgcccct cggggccgcc cagcacttcg tcgtcgtcgc cgccccagcc tatctggcgg
     3241 cccgcggtac gccggactgc ccgcgggcgc tgaccgccca cagctgcatc cgcgtgcgct
     3301 ttcccggcgg ccagtattac cgctgggaat tccggcgcgg cagcgagcag gtggagatcg
     3361 aagtggatgg ccccctcgcc accgacgaca tgcacgtcat gatcgccgcc gccgaagcgg
     3421 ggctgggcct cgcctacgtc tacgcccaat acgccgcccc ggccattgcc gccggccgcc
     3481 tcgtcagcgt gctggacgac tggcgcccgc cggagccggg cttcttcctc tactacccca
     3541 gccaccgcct gccgcccgcc ggcctgcgcg ccttcatcga tctggcgcgc cgcatccatc
     3601 cggacaaccg gccctgaacg acccggtccg cagcgcccgc ggctgccccc gcgcgcgcgg
     3661 atttgctatc gtcccaccat gcgccccctg ccccgccttg cctgcctcgc cctgctgctc
     3721 gcggctctgc tcgccgcctc ggcctacgcg gacgaactgc gccacgtcag cctccccgac
     3781 gcccgttacg cggacgcccg cgaggcgctg gtggacgcca tcgaaggcga aggcctgatc
     3841 ccttcgccgc ccagccgctt cggcgacatg ctggcgcgca ccggcccggc cctgggtcag
     3901 gacggcgccg tgtatagcga cgccgaagtg ctccatttct gcagcgcccg cgtagcctgg
     3961 caactggccc gggagaaccc cgtcaacatc gcccaatgcc ccctctccat ggccatctac
     4021 acccgcgcag gcgaggcggg cacggtgcat ctggcctggc gccccaccca gggcgacagc
     4081 gccggcagcc gcgccgccaa tgccctgctg gaacgcatcg ccgcccaggt aagcgacaac
     4141 accggccgcg cccgcccgcg ctgaggcgcg ccgcacccgt cacgcacgca tacgccacac
     4201 cctcgcgccg caacgatccc cgcggtatag tccgcgtcgc tccagactcc cacccgccgg
     4261 accgcgcatg acccaaaagc actacaccgc cgactccatc accgtcctca agggcctcga
     4321 acccgtcaaa cagcggccgg gcatgtacac ccgcaccgag cacccgctgc acgtcatcca
     4381 ggaagtcatc gacaacgccg ccgacgaagc catggccggc ttctgccggc gcatcggcgt
     4441 caccctgcac gccgacggct cggtgagcgt ggccgacgac gggcgcggca tccccgtcga
     4501 gatccacccg gtggaacgcg tctccacggt ggaagtggtg ttcacccgcc tccacgccgg
     4561 cggcaagttc aacaagaccg acgaaggctc cgcctaccgc ttctccggcg gcctgcacgg
     4621 cgtcggcgtg tccgtcacca acgccctcgc ccgccggctg gaagtggaag tcgtgcgcga
     4681 cggcggccga caccgcctgg tgtttgccgg cggcgacgtg atcgagcccc tcgtccgcat
     4741 cggcgacgcc cccaagaaag ccagcggcac caccgtccgc gtatggccgg acgccaaata
     4801 cttcgacgcc gccgagctgc cccgcggcga gctggaacgc ctgctccgct ccaaggccgt
     4861 actcatgccc ggcctggacg tcagcctcgc cgtagaaggc ggcgacaccc gcaactggcg
     4921 cttcgacgac ggcatgcgcg gctacctgaa cgaatccctc accggcgaac ccctcattcc
     4981 cctgttctcc ggcgaaaaat acgcgcccaa gggcgacgag accttcgccg ccggcgaagg
     5041 cgccgcctgg gccgtggcct ggaccgccga aggcgcgccg gtgcgcgaat cctacgtcaa
     5101 cctcatcccc acccccgccg gcggcaccca cgaagccggc ctgcgcgacg gcatcttcac
     5161 cgcggtcaag aacttcatcg accaccacgc cctgctcccc aaaggcgtca agctcaccgc
     5221 cgacgacgcc ttcgggcgcg cctccttcgt cctctccgcc cgcgtcctcg accccagctt
     5281 ccagggccag accaaggaac gcctcaacag ccgcgacgcc gtgcagctcg tctcccgcat
     5341 ggtgcgcgac cccttcgagc tgtggctcaa cgaacacgtg gagtacggca agaagctcgc
     5401 cgagctggcc atcaaggccg cccaggcccg cgcccgcgcc gcccagaagg tggaaaagcg
     5461 caagtcctcc ggcgtcgccg tgctgcccgg caaactgtcg gactgcgaat ccgaggacat
     5521 cgcccgcaac gaactcttcc tcgtggaagg cgactccgcc ggcggcagcg ccaagatggc
     5581 ccgcgacaa
//
LOCUS       DAOQPA010000168         5565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8809_length_5565_cov_16.311252, whole genome shotgun sequence.
ACCESSION   DAOQPA010000168 DAOQPA010000000
VERSION     DAOQPA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_5565_cov_16.311252"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PKX01_15870"
     CDS             <1..111
                     /locus_tag="PKX01_15870"
                     /inference="COORDINATES: protein motif:HMM:NF025245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HMZ77661.1"
                     /translation="ENFDAELGRKIARQNAVQKLWPLMGYALKERIASQA"
     gene            459..1433
                     /locus_tag="PKX01_15875"
     CDS             459..1433
                     /locus_tag="PKX01_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77662.1"
                     /translation="MSMTAAELLEAALDGTLQDDDATTEQPDAAVTDDATGDAAPEGQ
                     DKQDDASTETEVEGAPILSKSGAYTIPYEKLAEAREARKAAQDRIAQLEQQVAELTSA
                     QQRNLAAVQADAQDRANAGQAQTATDANLAAATQAMAEGVDIAVFGDFSEEDLAKGVA
                     ELNRRAMAQVEQRLMAALDDRLAPLRAQEAKTATSAHYDAIYAAHKDADEIVESAEFA
                     AWRDALPAFAKAGVEHALTKGAAKDVIEVFDAFRATKPPQPNTSTARSAPEAPVRRVP
                     NSLSDVPGAAPMDETQQTLAAAGNTAALLDRMAAMTQEQRDALLDNLI"
     gene            1454..2749
                     /locus_tag="PKX01_15880"
     CDS             1454..2749
                     /locus_tag="PKX01_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HMZ77663.1"
                     /translation="MTTKTNVPATAADKQRVLAAGLFAQAMQRNSTMGRLSGPMPKGE
                     AAAGEVVRKQTSTDLPIVKTMDLSRGKGDEVEFQFLQPVGAYPIMNSETAEGKGTGLS
                     YDTARVRVSQARFPVDLGDTMTTIRSAVDFRRLGRPVAQSLMDSYMDQSLLVHMAGAR
                     GFHDNIEWRIPVASHAKFAAMAVNPVKAPTKNRHFIADGTNGIIPFALSGGDVDMATT
                     DVLSMDVVDAIRTTMESIALPPPAVKIPGDVVAEDSPLRVLLVSPAQYHSFAQDPNFR
                     QFQANALARASKAKQHPLFLGECGLWNGVLIMKMPKPIRFYAGDAISYCTSHTAETEA
                     TATVPAGFGTTHAIDRAILLGGQAIAQAFAASGHGGMPFFWKEKDFDHGDKMELLIGA
                     IQGISKVRWLVDQGNGVKHYTDHGIVAIDTAVPIIAARQ"
     gene            2815..3297
                     /locus_tag="PKX01_15885"
     CDS             2815..3297
                     /locus_tag="PKX01_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77664.1"
                     /translation="MATITKLGIPDPSNQLGASPYGNLTAFRYVLETTATGAVKGGDS
                     TAAVASGDTVKIGLLPAGFRLIDSEIVVKTGLTATITAKVGFAYADGVDSTAVPQDDD
                     FFGTGITVATAGRYRNATTNTSVTLPKEAWLTLTTAVAANAKASELEIIVFAINEGVA
                     "
     gene            3356..3742
                     /locus_tag="PKX01_15890"
     CDS             3356..3742
                     /locus_tag="PKX01_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77665.1"
                     /translation="MQLIAVKYQGRKRYRDRTPLRNEWEPGDAKRVPERDAKTLLRFA
                     EFSRADEADITEQPGDAEAVIAAHAQREQDERTETESMLMLIDSMDKDALEAYALKYE
                     VNLDKRRGLDKLRAEVVSLIEQFGAR"
     gene            3744..4418
                     /locus_tag="PKX01_15895"
     CDS             3744..4418
                     /locus_tag="PKX01_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77666.1"
                     /translation="MTLAELIARYRTAAHDTILPGFCTDEELAEFYNDADAEAAIRGR
                     LLRTTAESHPSLCAIDVTAGDAVLSLHPAIYELSYQAWRATGATTRAPLALRTREWMD
                     ANVTDWRDMPADTPRWVIQEEDRLQLVPAPSVDGEVLLEAYRVPVDSMADDTDEPGIP
                     AIHHRHLVHWALYRAFSKPDGELFDPARAAQAEAEFSRYFGRRPDSDLRRDTREDEPQ
                     HVVAWL"
     gene            4608..4907
                     /locus_tag="PKX01_15900"
     CDS             4608..4907
                     /locus_tag="PKX01_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77667.1"
                     /translation="MMDSVRPTTEADRRHWHLDKTLNVSHLLTTIVIAGSLFAYANNM
                     DRRVAILEEKMQVQVQENSRSAAEVRELAADVKHELRLLRTELMRMMTEKAGGKP"
     gene            4992..5492
                     /locus_tag="PKX01_15905"
     CDS             4992..5492
                     /locus_tag="PKX01_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77668.1"
                     /translation="MDWKEVGVSIAAMAPALGVALGGPAGGAVGSLVAAAFGAKARPA
                     DVAAVIAGDPDAAVKLREIELRHAEVIASLAAQQYEAQLLDVQQARTAHQSHWMPGFL
                     TLLLAGMVASLGAALLTMEMPAENQEVLYLIAGQLLGAFATAIAYWLGSSRGSAEKQR
                     ALESRS"
BASE COUNT         1078 a         1954 c         1723 g          810 t
ORIGIN      
        1 gagaacttcg acgccgagct gggccgcaag atcgcccgcc agaacgccgt acagaagctg
       61 tggccgctga tgggctacgc gctgaaggaa cggatcgcca gccaagcgta gccaaagcgc
      121 cgagcgcagg cgccaccgcg ctgaacccaa gagagcccgc cgagcgcggg cttttttgcg
      181 cccgcatttc ccgtactacg ccctaatttc ccgcagggtt tcccgcacgc tttcccattt
      241 cccgcgtggg aatgccgcgc ctgccggatt tccacaaatc tccgcaggaa gctacccgtc
      301 atccgggtgc ttgctacgta gcgcgatagc gctgcgcgcg cctctgcgcc tctgcgctgt
      361 gcatccgccc aagccttacc ccctgtaggg ctggagcctt gtgcattcgc ccaaccaaac
      421 tgcccgcgca atctcgcacg cagcttggat aagcgcacat gagcatgacc gccgccgaac
      481 tcctggaagc cgccctggac gggacgcttc aggacgatga cgccaccacc gagcagccgg
      541 acgccgccgt caccgacgac gcgaccggag acgccgcacc cgaaggccag gacaagcagg
      601 acgacgccag caccgagacg gaagtcgagg gcgcgccgat cctctccaag tccggcgcct
      661 acaccatccc ctacgagaag ctggccgagg cccgcgaagc ccgcaaggct gcgcaggacc
      721 gcatcgcgca gctcgagcag caggttgccg agctgaccag cgcgcagcag cgcaatctcg
      781 ccgccgtgca ggccgacgcg caggaccgcg cgaacgccgg gcaggcacag accgcgaccg
      841 acgcgaacct cgccgccgcc acacaggcga tggccgaagg cgtcgacatc gcggtgtttg
      901 gcgacttctc cgaagaggac ttggcaaagg gtgtggccga gctgaatcgc cgcgcgatgg
      961 cccaggtcga gcagcgcctg atggccgcac tggatgaccg gctcgcaccg ctgcgtgcgc
     1021 aggaagccaa gacggcgacc agcgcccact acgacgccat ctacgccgcc cacaaggacg
     1081 cggacgagat cgtcgagtcg gccgagttcg ccgcgtggcg cgacgccttg cccgccttcg
     1141 ccaaggccgg cgtcgagcac gcactgacca agggcgcggc aaaggacgtg atcgaggtct
     1201 tcgacgcctt ccgcgctacc aagccgccgc aacccaatac cagcaccgca cgctccgcgc
     1261 cggaggcgcc tgtccgtcgt gtgccgaact ccctgtccga cgtgcccgga gcggcgccca
     1321 tggacgaaac gcagcagacc ctcgccgccg ccggcaacac cgccgccttg ctcgaccgca
     1381 tggccgcgat gacccaagag cagcgcgacg ccctgctgga caacctcatc tgaatccata
     1441 ggagtcatcg aacatgacca ccaagaccaa cgtcccggcc accgccgcgg ataagcagcg
     1501 cgtcctcgcc gcgggcctct ttgcccaggc gatgcagcgc aacagcacca tgggccgcct
     1561 gtccggcccc atgcccaagg gcgaggccgc agccggtgaa gtcgtgcgca agcagacttc
     1621 caccgatctg ccgatcgtca agaccatgga cctgtcgcgc ggcaagggcg acgaagtgga
     1681 attccagttc ctgcaacccg tcggcgccta cccgatcatg aacagcgaga cggccgaggg
     1741 caagggcacc ggcctgagct acgacaccgc ccgcgtgcgc gtcagtcaag cccgcttccc
     1801 ggtcgatctc ggcgacacca tgacgaccat ccgctcggcc gtggatttcc gccgcctggg
     1861 ccgcccggtc gcgcagtcgc tgatggacag ctacatggac cagtccctgc tggtgcacat
     1921 ggccggcgcg cgtggcttcc acgacaatat cgagtggcgc atcccggtcg cctcgcatgc
     1981 caagtttgcc gcgatggcgg tgaacccggt caaggcgccg accaaaaacc gccacttcat
     2041 cgccgacggg accaacggca tcatcccgtt cgcgctgtcc ggcggcgacg tggatatggc
     2101 aaccaccgac gttctgagca tggacgtggt cgacgcgatc cgcaccacca tggagtcgat
     2161 cgccctgccg ccgccggccg tgaagatccc cggcgacgtg gtggccgagg attcgccgct
     2221 gcgcgtcctg ctggtgagcc cggcgcagta ccactcgttc gcccaagacc cgaacttccg
     2281 ccagttccag gccaacgccc tggcgcgcgc gagcaaggcc aagcagcacc cgctgttcct
     2341 gggcgagtgc gggctgtgga acggcgtcct catcatgaag atgcccaagc cgatccgctt
     2401 ctacgcgggc gacgccatca gctactgcac cagccacacc gccgagaccg aggcgaccgc
     2461 gacggtgccg gccggcttcg gcacgaccca cgcgatcgac cgcgccatcc tgctcggcgg
     2521 ccaggcgatc gcccaggcgt tcgccgcatc gggtcatggt ggcatgccct tcttctggaa
     2581 ggagaaggat ttcgaccacg gcgacaagat ggagctgctg atcggtgcga tccagggcat
     2641 cagcaaggtg cgctggctgg tcgaccaggg caacggcgtg aagcactaca ccgaccacgg
     2701 catcgtcgcc atcgacaccg ccgtgcccat catcgccgcg cgccagtaac gcgaccacct
     2761 gagcaacagg gcgccgcctg acccggtggc gcccgccaag gagaactgaa caccatggct
     2821 accatcacca agctcggcat ccccgacccg tccaaccagc tcggcgcgtc gccctacggc
     2881 aacctgaccg cgttccgcta cgtgctcgag accaccgcga cgggcgccgt caagggtggc
     2941 gactcgaccg cggccgtcgc ttcgggcgac accgtgaaga tcggccttct gccggcgggc
     3001 tttcgcctga tcgacagcga gatcgtcgtc aagaccggcc tcaccgccac catcaccgcc
     3061 aaagtgggct ttgcctacgc cgacggcgtg gattcgacgg ccgtgccgca ggacgacgac
     3121 ttcttcggca ccggcatcac cgtggcgacc gccggccgct atcgcaacgc gaccaccaac
     3181 accagcgtga cgctgccgaa agaggcgtgg ctgacgctga ctaccgctgt ggcggccaac
     3241 gccaaggcgt cggagctgga aatcatcgtc ttcgccatca acgaaggcgt ggcgtaactg
     3301 ccatgaatcg gccggctggg cgatccccgg ctggccgatt gcacgagagg acagcatgca
     3361 actcatcgcc gtgaaatacc agggccggaa gcgctatcgg gaccgcacgc cgctgcgcaa
     3421 cgagtgggag ccgggtgacg ccaagcgcgt gcccgagcgc gacgccaaga cgctcctgcg
     3481 ctttgccgaa ttcagccgcg ccgacgaagc ggacatcacc gagcagcccg gcgacgccga
     3541 ggccgtcatt gcagcccatg cccagcgtga gcaggacgag cgcaccgaga ccgaatccat
     3601 gctgatgctg atcgacagca tggacaagga cgcgctcgag gcctacgcgc tcaagtacga
     3661 agtcaacctg gacaagcgcc gtgggctcga caaactgcgc gccgaagtcg taagcctgat
     3721 cgagcaattc ggagcgcgct gacatgacgc tggccgagct gatcgcgcgc taccggactg
     3781 ccgcacacga cacgatcctg ccggggttct gcaccgacga agagctggcc gagttctaca
     3841 acgatgccga tgccgaggcc gcaattcgtg gtcgactgct gcgcaccacg gcggaatccc
     3901 atccgtcgct gtgcgcaatc gacgtgacgg ccggagacgc cgttctgagc ctgcatccag
     3961 cgatctacga actgagctat caggcatggc gcgcgacggg cgcaacgacc cgcgcccccc
     4021 tggcactgcg cacgcgcgaa tggatggacg ccaacgtcac cgactggcga gacatgccgg
     4081 ccgatacccc gcgatgggtg attcaggaag aagacagatt gcagcttgtt cccgccccat
     4141 ccgtggatgg cgaagtgctg ctcgaagcct accgcgtccc ggtcgattcc atggccgacg
     4201 acaccgacga gccgggcatc cctgcgatcc atcatcgcca ccttgttcat tgggcgctct
     4261 accgcgcatt cagcaagccg gatggggagc tattcgaccc tgctcgggct gcacaggctg
     4321 aagccgaatt ctcgcgctac ttcggtcgcc ggccggattc ggaccttcgc cgggacacgc
     4381 gcgaagacga gccgcagcat gtcgtcgcat ggctataggc cccctgtagg gctggatgat
     4441 cgacggcatg gcggcaacac tgcggcaaaa ccagtttgga ctgttgccat gccgaaaatc
     4501 atcctcgacc acaaaccatc cgaaaccccg gcgatcggat cggcagcagt cctccccgaa
     4561 tttcccgccc gctctgcata ttgcgaggcg atgcacagcg gagcgaaatg atggacagtg
     4621 ttcgccccac aaccgaagcc gaccgcaggc attggcacct cgataaaacc ctcaacgtct
     4681 cccatcttct tacgaccatt gtcatcgccg gcagtctctt cgcctacgcg aacaacatgg
     4741 atcggcgcgt ggcgatcctc gaagagaaga tgcaggttca ggtgcaggaa aacagccgat
     4801 ccgccgcaga ggtgcgagag ctggcggcag acgtgaagca tgaactgcgg ctactgcgta
     4861 ccgaactcat gcggatgatg accgaaaaag ccgggggcaa gccgtgaatt tcgagcacgc
     4921 ctttgaaaag tgggatcact ttggccgcgg atggtcccgc cgcatcgcat ccaacctgct
     4981 cggagcatga catggactgg aaagaggttg gcgtctcgat tgctgcgatg gccccggcgc
     5041 ttggcgtggc gcttggcggt cctgctggcg gtgctgttgg ttcgctggtg gctgcggcat
     5101 ttggcgcgaa agcccggccc gccgacgtgg ctgcggtgat cgcaggcgac ccggacgctg
     5161 cggtgaagct gcgcgagatc gaactacggc acgccgaggt catcgcatcg ctcgcagcgc
     5221 agcagtacga ggcgcaattg ctcgacgtgc agcaggcgcg cacggcccat caaagccact
     5281 ggatgcccgg cttcctgact ctgctccttg ccggcatggt cgcaagcctt ggcgcggcgc
     5341 tccttaccat ggagatgccc gccgaaaatc aggaagtgct gtacctcatt gcggggcaac
     5401 tgctcggcgc attcgccacc gcaattgcct actggctcgg gtcttctcgc ggctcggcgg
     5461 aaaaacagcg cgcactggag tcccgctcat gagctaccag gaactcccga tggcatgccg
     5521 ccagtgtgcg caccggcaca gccagtacat ctacccggcc tggag
//
LOCUS       DAOQPA010000169         5552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8840_length_5552_cov_16.634346, whole genome shotgun sequence.
ACCESSION   DAOQPA010000169 DAOQPA010000000
VERSION     DAOQPA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8840_length_5552_cov_16.634346"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKX01_15910"
     CDS             <1..531
                     /locus_tag="PKX01_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77669.1"
                     /translation="PPALVIAAARAALAPVVQAFSAARLDLAAIDVPELALRNIAALH
                     NTAPRGIVLLHFDADGGLLVVCHEGELVTSRRLDVSADQLALADDLRVSRYERLGLEL
                     QRSLDNFDRQYSHIPLERLVLSVPPEAASIIDFLASNLYIPVEPLDLGALMGGTGQLG
                     ACSLLTLGAALRSEPA"
     gene            528..1241
                     /locus_tag="PKX01_15915"
     CDS             528..1241
                     /locus_tag="PKX01_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77670.1"
                     /translation="MSREINLYDASLRPVRDPYTLPALLLAAGAALALIVGGHLLARV
                     QLDDARRAAVDSEARMSALRDRLVEDSRAIAGHVADTALQQRLDEREAELQRLRLADA
                     QIREVLKDQQAPFAEVLRALARRDTDGIWLDSVEIGPGARLTRLQGQTTHEALIPDYL
                     ARLGQEVPLAGLAFAQLAIDMPAEAPAAGKPLADPAAAARAAAGKPAAPPPPPAERPR
                     RFTLSARLAATAPAREAPR"
     gene            1238..1909
                     /locus_tag="PKX01_15920"
     CDS             1238..1909
                     /locus_tag="PKX01_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77671.1"
                     /translation="MKRQWTQACARFAALARRERLLVSSAILVGILMLGYTYAIEPQL
                     IQRDKYRQSVALHEDETRRIGEQAALLARQAADPERAKRDALARAEAEGATLRGQVHD
                     LDRLLVTPAAMEAVLTDLLHRRPGVRVLSLVTLPPQPAVARPAGEGDANAKDAAPQGL
                     FRHTLRLQVEGTYAELTALLAELDKGPTRLFRERAVLTADGAGRCRLELELFTLGLNP
                     SWLAL"
     gene            1894..2313
                     /locus_tag="PKX01_15925"
     CDS             1894..2313
                     /locus_tag="PKX01_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77672.1"
                     /translation="MARPVTAPLRRLAALAAILAAPLAVAQALPDPTRPPAGWGPGES
                     PAIAAPAGARLQMIVRSPGEAPWALIDGQRVRIGERVDGARLIRLTDTEAVLDDSGTT
                     RRLSLSPGLIRPVAPQAAPAVPSSAASRNAPSQETRR"
     gene            2310..4001
                     /gene="mshL"
                     /locus_tag="PKX01_15930"
     CDS             2310..4001
                     /gene="mshL"
                     /locus_tag="PKX01_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HMZ77673.1"
                     /translation="MKLPALAVALVAGAAVLSGCSLHTPTKATYSQIEQEVQAAAESR
                     ARSVEPAPAAAPVAPPPRREPDKRFDVAFSNAPAAQVFAALGGSSRYNLLVHPEVAGT
                     ISAHLKNVTLFEALDALRDLYGYDFRVQGNRVTVLPATLQSRIFQINYLAGRRQGQTD
                     VRVTSGSIASSPSANTTGVPSAVPNPTVPNTPGATGAQARAPDSSRVQTSSDQDFWGD
                     ISRSLQIIVGSEAGRSVVINSGSGVILVRAMPGELQQVEQFLRATQLVIERQVMLEAK
                     IVEVQLSEGFQSGINWANLEKGYGVGLDPREINIRSGVSTSTTLGSLLGSGLSSGTGR
                     TDAGIFGIAVKTGNFAALLQFLETQGNVQVLSSPRIATLNNQKAVLKVGTDEFFVTNV
                     TTTTNSSSATTTTSPTITTAPFFSGIALDVTPQIDVDGNIILHIHPSISAVTEKAKNI
                     NLGQLGSITLPLASSNVNETDSIVRIRDGQIVAIGGLMKQTQSDSRSAVPVGGDIPVI
                     GFLLGQRNKSFVKSELVIMLKPTVIRSGDDWRPDLEGFHLRMQDYVPPERDVLRP"
     gene            complement(4136..5551)
                     /locus_tag="PKX01_15935"
     CDS             complement(4136..5551)
                     /locus_tag="PKX01_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HMZ77674.1"
                     /translation="MRALQAGAQPTRPFQRVSMIRFAPLRCGMSSCRVRPLAGLLALF
                     ALGRAAHAAEGDYETWTARVQGTYVTQYKPSFPAAYSGRNSLSADYEHSYTATMTAYM
                     GFRPWRGGELYLDPELTEGVPFSGLNGSAGFTNGELTRTAGKKPKLYRQRLFLRQTWG
                     LGGGEEAVEADLNQMAGAVDKNRVVLTVGNFSTLDVFDDNAYAKDPRVHFMNAAFMAP
                     LAYDYAADARGFGWGFALEWYQDVWALRIGRMTGPRDPNMLPLDFHLASHYGDQVEVE
                     HAHTWSGQPGKVRVLAFRNRARIARFDDALAYLRANPAADPQTIFAVRDRDQVKYGVG
                     VNLEQAINADVGVFLRAMRADGQTETLAFTETDTSVAGGVSVKGRAWGRGEDTVGVGL
                     SRNMLSSARRAYLAAGGLSFFIGDGALNYRPEDLAEAYYSLRLAKGVFLTADMQRMSH
                     PAYNADRGPANFYALRLHGEF"
BASE COUNT          866 a         2375 c         1591 g          720 t
ORIGIN      
        1 ccgcccgccc tcgtgatcgc tgccgcccgc gcggcgctgg cgcccgtggt gcaggccttc
       61 agcgccgccc gcctcgatct ggcggccatc gacgtgcccg aactggccct gcgcaacatc
      121 gccgccctcc acaacaccgc gccgcggggc atcgtgctcc tccacttcga cgccgatggc
      181 ggcctgctgg tggtctgcca cgaaggcgaa ctcgtcacct cgcgccgcct cgacgtctcc
      241 gccgaccagc tcgccctcgc cgacgacctg cgcgtctccc gctacgaacg cctgggcctg
      301 gagctgcaac gctccctcga caacttcgac cgccagtaca gccacattcc cctcgaacgc
      361 ctggtcctct cggtgccccc cgaagccgcc agcatcatcg acttcctggc ctccaacctg
      421 tacatccccg tcgaacccct cgacctcggc gcgctgatgg gcggcaccgg gcagctcggt
      481 gcctgctccc tcctcaccct cggcgcggcc ctgcgctcgg agcccgcatg agccgcgaga
      541 tcaacctcta cgacgcgagc ctgcgccccg tccgcgatcc ctacaccctg cccgccctcc
      601 tgctggcggc cggggccgcc ctcgccctga tcgtcggcgg ccatctcctc gcccgcgtcc
      661 agctcgacga cgcccgccgc gccgccgtcg acagcgaagc ccgcatgagc gccctgcgcg
      721 accgcctggt ggaggattcc cgcgccatcg ccggccacgt ggccgacacc gccctgcaac
      781 agcgcctcga cgaacgggag gccgaactgc agcgcctgcg cctcgccgac gcacagatcc
      841 gcgaagtcct caaggaccag caggccccct tcgccgaggt gctgcgcgcc ctcgcccgcc
      901 gcgacaccga cggcatctgg ctcgacagcg tcgagatcgg ccccggcgcg cgcctcaccc
      961 ggctgcaggg ccagaccacc cacgaagccc tgatccccga ctacctcgcc cgcctcggtc
     1021 aggaagtccc cctcgccggc ctggcctttg cccagctggc catcgacatg cccgccgaag
     1081 cccccgccgc cggcaagccc ctcgccgatc cggccgcggc ggcccgagcg gcggccggca
     1141 aacccgccgc accgcctccg ccgcccgccg aacgcccccg ccgcttcacc ctgtccgccc
     1201 gactggccgc cacggccccc gcccgggagg cgccgcgatg aagcgccaat ggacccaagc
     1261 ctgcgcccgc ttcgccgccc tcgcccggcg tgagcgcctg ctggtgagca gcgccatcct
     1321 ggtcggcatc ctcatgctcg gctacaccta cgccatcgag ccccagctca tccagcgcga
     1381 caagtaccgc cagagcgtcg ccctgcacga agacgaaacc cgccgcatcg gcgaacaggc
     1441 cgccctcctc gcccgccagg ccgccgatcc cgagcgggcc aagcgagacg ccctggcccg
     1501 cgccgaagcc gaaggcgcca ccctgcgcgg ccaggtccac gacctggacc gcctgctggt
     1561 cacccccgcc gccatggagg cggtgctcac cgacctgctc caccgccgcc ccggcgtgcg
     1621 cgtgctgtcc ctcgtcaccc tgccgcccca gcccgccgtg gcccgcccgg cgggcgaggg
     1681 cgacgccaac gccaaagacg ccgcgccgca aggcctcttc cgccacaccc tgcgcctgca
     1741 ggtggaaggc acctacgccg agctcaccgc cctgctggcc gagctggaca agggcccgac
     1801 ccgcctcttc cgcgaacggg ccgtgctcac cgccgacggc gccggccggt gccgcctcga
     1861 actggaactg ttcaccctcg gactgaaccc gtcatggctc gccctgtgac cgccccattg
     1921 cgccggctcg cggccctcgc ggcgatcctc gccgcccccc tcgccgtggc ccaggccctg
     1981 cccgacccca cccgtccgcc tgccggctgg ggccccggcg aatcgcccgc catcgccgca
     2041 ccggcgggcg cccgcctgca gatgatcgtg cgcagccccg gcgaagcccc gtgggccctc
     2101 atcgacggcc agcgcgtacg catcggcgag cgcgtcgatg gcgcccgcct catccgcctc
     2161 acagacaccg aagccgtcct cgacgacagc ggcacgaccc gccgcctgag cctctcgccg
     2221 ggcctcatcc gccccgtcgc gccccaggca gcccccgccg tgccgtcctc cgctgcatcc
     2281 cgtaacgccc cgtcccagga gacccggcga tgaaacttcc cgccctcgcc gtcgccctcg
     2341 tcgccggtgc cgccgtcctc tccggctgct ccctgcacac gcccaccaag gccacctaca
     2401 gccagatcga gcaggaggtc caggccgccg ccgaatcccg cgcccgcagc gtcgagcccg
     2461 ccccggccgc cgcacctgtc gcgccgccgc cccgccgcga acccgacaag cgcttcgacg
     2521 tcgccttcag caacgccccc gccgcccagg tcttcgccgc cctcggcggc agctcccgct
     2581 acaacctgct ggtgcacccc gaagtcgccg gcaccatcag cgcccacctc aagaacgtca
     2641 ccctcttcga agccctcgac gccctgcgcg acctctacgg ctacgacttc cgcgtgcagg
     2701 gcaaccgggt caccgtgctg cccgccaccc tccagtcgcg catcttccag atcaactacc
     2761 tggccggccg ccgccagggc cagaccgacg tgcgcgtcac ctccggctcc atcgccagca
     2821 gccccagcgc caacaccacc ggcgtgccca gcgcggtgcc caaccccacc gtgcccaaca
     2881 cccccggcgc caccggcgcc caggcccgcg cgcccgacag cagccgcgtg cagacctcct
     2941 ccgaccagga cttctggggc gacatcagcc gctccctgca gatcatcgtc ggcagcgagg
     3001 ccggccgcag cgtcgtcatc aacagcggct ccggcgtcat cctggtgcgc gccatgcccg
     3061 gcgaactgca gcaggtggag cagttcctgc gcgccaccca gctggtcatc gaacgccagg
     3121 tcatgctcga agccaagatc gtcgaagtgc agctctccga aggcttccag tccggcatca
     3181 actgggccaa cctggaaaaa ggctacggcg tcggcctcga cccgcgggag atcaacatcc
     3241 gctccggcgt gagcaccagc accaccctgg gcagcctgct cggcagcggc ctcagcagcg
     3301 gcaccgggcg caccgacgcc ggcatcttcg gcatcgccgt caagaccggc aacttcgccg
     3361 ccctgctcca gttcctcgaa acccagggca acgtgcaggt gctctccagc ccgcgcatcg
     3421 ccaccctcaa caaccagaag gccgtgctca aggtgggcac cgacgaattc ttcgtcacca
     3481 acgtcaccac caccaccaac agcagcagcg ccaccaccac cacctcgccc accatcacca
     3541 cggcgccctt cttctccggc atcgccctcg acgtcacacc ccagatcgac gtggacggca
     3601 acatcatcct gcacatccac ccgtccatca gcgccgtcac cgaaaaggcc aagaacatca
     3661 acctcggcca gctcggctcc atcaccctgc cgctggcctc cagcaacgtg aacgagaccg
     3721 actccatcgt gcgcatccgc gacggccaga tcgtcgccat cggcggcctc atgaagcaaa
     3781 cccagtccga ttcccgcagc gccgtgccgg tgggcggcga catcccggtg atcggcttcc
     3841 tcctcggcca gcgcaacaag agcttcgtca agagcgagct ggtcatcatg ctcaagccca
     3901 ccgtcatccg ctccggtgac gactggcgcc cggacctgga aggcttccac ctgcgcatgc
     3961 aggactacgt cccgccggaa cgggacgtgc tgcgcccctg acacaccgcg cccccctcaa
     4021 aacacaaagc cccggtcgag ccggggcttt gtcattgaag gcgccgccgg cacgcaccgg
     4081 ccgccgcggg cgacgcgccc taccggcgca tcgcccccat ccccgccgcc cgccttcaga
     4141 actccccgtg caggcgtagc gcgtagaaat tggccgggcc acggtccgcg ttgtaggccg
     4201 ggtggctcat gcgctgcatg tccgcggtca ggaacacccc cttcgccaga cgcaggctgt
     4261 aatacgcctc cgccagatcc tccggccggt agttgagcgc cccgtcgccg atgaagaagg
     4321 acagcccgcc cgccgccaga taggcccggc gcgcgctcga cagcatgttg cgggacaggc
     4381 ccacgcccac cgtatcctcg ccgcgccccc aggccctacc cttcaccgac acaccgcccg
     4441 ccaccgacgt atccgtctcg gtgaaggcca gcgtctccgt ctgcccgtcc gcccgcatgg
     4501 cccgcaggaa gacgcccacg tccgcgttga tcgcctgctc caggttgacc cccacgccgt
     4561 acttcacctg gtcccggtcg cgcaccgcaa agatcgtctg cgggtccgcc gccgggttgg
     4621 cgcgcagata ggccagcgca tcgtcgaagc gggcgatgcg cgcccggttg cgaaacgcca
     4681 gcacgcgcac cttgcccggc tggccggacc acgtgtgggc gtgctccacc tccacctgat
     4741 cgccgtaatg gctcgccaga tgaaaatcca ggggcagcat gttcggatcc cgcgggcccg
     4801 tcatgcgccc gatgcgcagc gcccacacat cctgatacca ctccagcgca aagccccagc
     4861 cgaagccccg cgcatccgcc gcgtagtcat aggccagcgg cgccatgaag gccgcgttca
     4921 tgaaatgcac ccgcggatcc ttggcgtagg cgttgtcgtc gaacacatcc agcgtggaaa
     4981 agttgcccac cgtcagcacc acccggttct tgtccaccgc gcccgccatc tggttcagat
     5041 ccgcctccac cgcctcctca cccccgccca ggccccaggt ctggcgcagg aacaggcgct
     5101 ggcgatacag cttgggcttc ttgcccgccg tgcgcgtcag ctccccgttg gtgaagccgg
     5161 cgctgccgtt gagccccgag aacggcaccc cctcggtgag ctccggatcc agatacagct
     5221 cccccccgcg ccacggccga aaccccatgt aagccgtcat cgtggccgtg taactgtgct
     5281 cgtagtccgc cgacagactg ttgcgcccgc tgtaggccgc cgggaaggac ggcttgtact
     5341 gggtgacata cgtcccctgc acccgcgccg tccacgtctc gtagtcaccc tcggccgcgt
     5401 gggccgcgcg ccccagggcg aacagcgcga gaagaccggc aaggggacgg acacgacatg
     5461 aagacatgcc gcagcgcaat ggcgcgaatc ggatcatgga gactctctgg aagggacggg
     5521 tcggctgtgc gccggcctgc agcgcgcgca tc
//
LOCUS       DAOQPA010000170         5530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8897_length_5530_cov_19.366758, whole genome shotgun sequence.
ACCESSION   DAOQPA010000170 DAOQPA010000000
VERSION     DAOQPA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_5530_cov_19.366758"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PKX01_15940"
     CDS             <1..1068
                     /locus_tag="PKX01_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HMZ77675.1"
                     /translation="GRYLFVIGRDAKINMIDLWMEKPDNVAEIRTGLEARSVETSKFK
                     GYEDKLAVAGSYWPPQYVIMKGDTLEPLKIMATRGMTVDDQEFHPEPRVASIVASHYK
                     PEFVVNVKETGKTLMVNYTDINAITTKEIPVAKFLHDGGLDSSKRYFMVAANQSNKIG
                     AIDLKEGKLAGLIEVGKIPHPGRGANFIHPKYGPVWSTGHLGDDTIALIGTDPVKHPQ
                     YAWKMVESIKGPGGGALFIKSHPKSKHLYSDTPLNPDPKLSQSVVVFDTNNLAAGYKT
                     LPIGEWAGLKDDGAKRVVQPEFNKAGDEVWFSVWSAKNKESAIVVVDDKTLQLKTVIK
                     DPRLITPTGHFNVYNTQHDIY"
     gene            1139..1450
                     /locus_tag="PKX01_15945"
     CDS             1139..1450
                     /locus_tag="PKX01_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ77676.1"
                     /translation="MKRSLFLTVLLATASTAAFADAPAAAQKAGCMACHATDKKVVGP
                     AFKDIAAKYKGQADAVAKLSDKVRKGGAGVWGPIPMPPNAADKINDADLKSTVEWILK
                     G"
     gene            complement(1520..2566)
                     /locus_tag="PKX01_15950"
     CDS             complement(1520..2566)
                     /locus_tag="PKX01_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HMZ77677.1"
                     /translation="MTQRIEWIDTAKGIGIYLVVTGHLLANNPPDSRLAEHLIWAFHM
                     PLFFFLSGLTHRPAAPGRVMEEISRTAKAIGMPYLFFSLLSIALWWTGTPADARGHGL
                     DDAFATLLSGVSGREKGLFYNIPLWFFPCLFSVRMLAVLAGAMLGENRTRLLVAACGL
                     AALAHLVLFPVLPRLPWNLDVAIVAGVFYAAGHRLAGTPALDSLLTHRFVAPVAGMSL
                     LLGGCLLNGRVDMNYRDFGQPLLFYVAAAGGIATTLWLSKRLEALRWLRTVGVASVVI
                     FPMHGLLQYFLPGNALATLTWYAFRATGSGVVGALLMGVLEVAMCLPFYGLLARHAPV
                     CIGRFPRRAARVAS"
     gene            2881..3225
                     /locus_tag="PKX01_15955"
     CDS             2881..3225
                     /locus_tag="PKX01_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ77678.1"
                     /translation="MDRTLLLHVALLLGLTALAVSAAPAAEPDGARQRALVHLVRQDC
                     GSCHGMRLSGGLGPDLRPARLAALPDESLVATILYGRPGTAMPGWQRFLSADEAGWIV
                     AQLKQGFPPLEP"
     gene            3226..4413
                     /locus_tag="PKX01_15960"
     CDS             3226..4413
                     /locus_tag="PKX01_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HMZ77679.1"
                     /translation="MKTLHWLSVAALALGLTACAAPASRCDELRGTGDLGVIIERASG
                     QVQVVEHSGRTSLGRVDGLGDLSHAHVAFSRDGRYAYVFGRDGGLTKVDLLTRRIARR
                     VVQAGNSIGGAVSQDGRVVVAQNYEPGGIKAFDADTLELLADVPAEVAPGRFSRVVGL
                     ADAPGRRFVYALFDGDEIRVSDFSDPRHPVTTRYPAGRQPYDGLVTPDGRYFLAGLFG
                     EDGVSLLDLWQPEKGVTRILAGYGRGEEKLPVFKMPHLRGWSLAAGQAWLPAVGRHEV
                     LAVDTRTWAETARIPVKGQPVFVMARPDGRQIWVNFAFPDNDWVQVIDTVERRVIHSF
                     SPGKAVLHLEFTPRGEEVWISSRDSDRVSVVDTATFATRTELPAAAPSGIFFTARGQR
                     IGF"
     gene            4417..5415
                     /locus_tag="PKX01_15965"
     CDS             4417..5415
                     /locus_tag="PKX01_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ77680.1"
                     /translation="MLTADARDFHLLNDFQRGFPLRAEPWAALAAHLGCGADEVMQRL
                     ARLRDSGKISRVGPVFAPNRLGASTLAAMAVPPAELPRVAALVSAFAGVNHNYRREHR
                     FNLWFVAAAADERALNGLLAAIGSATGCPPVALPLVEEFHIDLGFDLANGRRTRHKPA
                     PVGMKRALSAAEWRLVEALQDGLPWVPRPYRAVADAAGLNEEDVLATLAAWLAEGTLR
                     RFGVVVRHRELGWDANAMCVWAVPEAAVSAAGRALAAQDGVSLCYRRRTAPEWPYSLF
                     CMLHGRSRAEVEARRMTVADAAGVAGYPSAVLFSTDCYKQCGARYRDATPQPACVS"
BASE COUNT          907 a         1903 c         1882 g          838 t
ORIGIN      
        1 ggccgctacc tgttcgtgat cggccgcgac gccaagatca acatgatcga cctgtggatg
       61 gagaaacccg acaacgtcgc cgagatccgt accggtctcg aagcccgctc ggtggaaacc
      121 tccaagttca agggctatga agacaagctg gccgtcgccg gctcctactg gccgccccag
      181 tacgtgatca tgaagggcga caccctcgag ccgctgaaga tcatggccac ccgcggcatg
      241 accgtggatg accaggagtt ccaccccgag ccgcgcgtgg cctccatcgt ggcctcgcac
      301 tacaagcccg agttcgtggt gaacgtgaag gagaccggca agacgctgat ggtcaactac
      361 accgacatca acgccatcac caccaaggaa atcccggttg ccaagttcct gcacgacggc
      421 ggcctcgaca gctccaagcg ctacttcatg gtcgccgcca accagtccaa caagattggc
      481 gcgatcgacc tgaaggaagg caagctggcc ggcctgatcg aagtgggcaa gatcccgcac
      541 ccgggccgcg gcgccaactt catccatccg aagtacggcc ccgtgtggtc caccggtcac
      601 ctgggcgacg acaccatcgc cctgatcggc accgacccgg tcaagcaccc gcagtacgcc
      661 tggaagatgg tcgagtcgat caagggcccg ggcggcggcg cgctgttcat caagagccac
      721 ccgaagtcca agcacctgta ttccgacacc ccgctcaacc cggatcccaa gctgtcccag
      781 tcggtggtcg tgtttgacac caacaatctg gcggccggtt acaagaccct gcccatcggc
      841 gaatgggccg gtctgaagga tgacggcgcc aagcgcgtgg tgcagccgga gttcaacaag
      901 gcgggcgacg aggtctggtt ctcggtgtgg agcgccaaga acaaggaatc cgccatcgtg
      961 gtggtggacg acaagaccct gcagctcaag accgtcatca aggatccccg tctgatcacc
     1021 ccgacgggtc acttcaacgt gtataacacg cagcacgaca tctactgatc gtcgtgccgt
     1081 ccgcggccgg ttcgatccgg ccgcggtcct gccaacatgt cagatgggag aataaactat
     1141 gaagcgttcg ctgtttctga ccgtcctgct tgccaccgcc agcactgcgg cgtttgcaga
     1201 tgcacctgcc gccgcccaga aggctggctg catggcctgt catgccaccg acaagaaggt
     1261 cgtgggtccc gcgttcaagg atattgccgc caagtacaag ggccaggccg acgccgtcgc
     1321 caagctgagc gacaaggtcc gcaagggcgg cgccggtgtg tggggtccga tccccatgcc
     1381 gcccaacgct gccgacaaga tcaacgacgc cgacctgaag tccaccgtgg agtggatcct
     1441 gaagggttga ggcgtgcctg atcggccgga tgcggggcgg cactggccgc cccgttccgt
     1501 ttcatccggg ccaatcgatt cacgacgcga cccgtgccgc acggcgcggg aaacgcccga
     1561 tgcacaccgg tgcatggcgc gccagcagtc cgtagaacgg caggcacatc gccacttcca
     1621 gcacacccat gagcagcgcc cccacgacgc ccgagccagt cgcccggaag gcataccagg
     1681 tgagggtcgc cagggcgttg ccgggcagga aatactgcag cagcccgtgc attggaaaga
     1741 tcaccaccga cgcgacgccg acggtccgca gccagcgcag cgcttcgagg cgctttgaca
     1801 gccacagggt ggtggcgatc ccgccggcgg ccgcgacgta gaacagcagg ggctgaccga
     1861 agtcccggta gttcatgtcc acccgcccgt tgaggagaca gccgcccagc agcaggctca
     1921 tgccggccac gggtgccacg aaccggtggg tcagcaggct gtccagtgcc ggcgttccgg
     1981 ccagccgatg gcctgcggca tagaacacgc ccgcgacaat ggccacatcc aggttccagg
     2041 ggaggcgcgg cagcaccggg aacaggacca ggtgggcaag ggctgcgagg ccgcaggccg
     2101 ccacgaggag gcgcgtgcga ttctcgccga gcatggcgcc cgccagcacg gccagcatcc
     2161 ggacgctgaa caggcaggga aagaaccaca ggggaatgtt gtagaagagc cctttttcgc
     2221 gtcccgacac gccagagagc agggtcgcga aggcgtcgtc cagtccgtgt ccgcgggcgt
     2281 cggccggcgt gccggtccac cacagggcga tcgacagcag gctgaagaac aggtagggca
     2341 tcccgatggc tttcgccgtc cgcgagatct cctccatcac ccggcccggc gccgccggac
     2401 ggtgcgtgag gccggacagg aagaagaaca gcggcatgtg gaaagcccag atcagatgct
     2461 ctgccaggcg cgaatccggt ggattgttgg cgagcaggtg gccggtgacc accaggtaga
     2521 tgccgatccc tttggcggtg tcgatccact cgattcgttg cgtcatgggg tgttcggtcg
     2581 gcgcggggcc gtgctgcagg aaggaaaggg cggcggaggg cgccgggaca aggccggccg
     2641 gaacgtatgc tacctcagct cgggacgctg gcgtcggcct gcgggttgcg ggccgagcgc
     2701 gcgccggaca cccgcgggtt cggggtggcg ccccgccgct ttcggccact gccgagatcg
     2761 tcaaggatcg gtccatgccg gaggcttcgc aggcctgccc gcccgccctt gatgcctgtc
     2821 aaggcaggcg accgggcctt gaccgtaccg tacgcccatc gaattgcgga tagcctgtcc
     2881 atggaccgta ccctgctcct ccacgtggcc ctgctgcttg gcctgacggc cctggcggtg
     2941 agtgccgcgc cggccgccga accggacggc gcacgccagc gggccctggt acatctggta
     3001 cgccaggact gcggctcctg ccacggcatg cgcctgtccg gcggactcgg ccccgatctg
     3061 cgtccggctc ggctggccgc cctgcccgac gagtccctgg tggcgaccat cctgtacggc
     3121 cgtcccggca ccgccatgcc gggctggcaa cgcttcctca gcgcagacga ggcaggctgg
     3181 atcgtggcgc agctcaaaca gggctttccg cccctggagc cctagatgaa gacccttcac
     3241 tggctttccg tcgctgcgct ggcccttggc ctgacggcct gcgccgcgcc cgcgtcgcgc
     3301 tgcgatgaac tgcgcggcac cggcgatctg ggcgtgatca tcgaacgggc cagcggccag
     3361 gtgcaggtgg tcgagcatag cgggcgtacg tccctgggcc gcgtggatgg cctaggcgac
     3421 ctctcccacg cccacgtggc cttttcccgg gacgggcgct acgcctacgt gttcggccgc
     3481 gatggcgggc ttaccaaggt ggatctgctc acccggcgca tcgccaggcg ggtggttcag
     3541 gctggcaact ccatcggcgg cgccgtgtcc caggacggtc gcgtggtggt ggcccagaac
     3601 tacgagcccg gcggcatcaa ggccttcgat gcggacaccc tggaactcct cgccgacgtg
     3661 cccgccgagg ttgcgccggg caggttctcc cgcgtggtgg ggctggcgga tgcaccgggg
     3721 cggcgcttcg tgtatgccct cttcgacggc gacgagatcc gcgtgagcga tttcagcgac
     3781 ccgcgccatc cggtgaccac ccgctacccg gccggccggc agccctatga cgggctggtg
     3841 accccggacg gacgctactt cctggccggg ctcttcggcg aggacggagt ctctctcctc
     3901 gacctgtggc agcccgaaaa gggggtcacc aggatcctcg ccggctatgg ccgcggcgag
     3961 gagaagctgc ccgtcttcaa gatgccccac ctgcgcggct ggtccctggc ggcggggcag
     4021 gcgtggctgc cggcggtggg ccgtcacgag gtgctggcgg tggacacgcg cacctgggcg
     4081 gagaccgcgc gcatccccgt caagggccag ccggtgttcg tcatggcgcg tccggacggt
     4141 cgccagatct gggtgaattt tgcctttccc gacaacgact gggtgcaggt catcgacacc
     4201 gtggagcgcc gcgtgatcca cagcttcagc cccggcaagg cggtgctgca cctggaattc
     4261 acgccgcggg gcgaggaagt gtggatttcc agccgcgaca gcgaccgggt cagcgtggtg
     4321 gacaccgcca ccttcgcgac gcgcaccgag ctgcccgcgg cggcaccgtc cgggatcttc
     4381 ttcacggccc gcggccagcg catcggattc tgaatcatgc tcaccgcaga cgcccgcgac
     4441 ttccacctgc ttaacgactt ccagcgcggc tttccgctgc gtgccgagcc ctgggcggcg
     4501 ctcgccgccc acctgggctg cggtgccgac gaggtgatgc agcgcctcgc ccggttgcgc
     4561 gattcgggca agatcagccg cgtcgggccg gtctttgcgc ccaaccgtct gggtgccagc
     4621 accctcgcgg cgatggcagt gccaccggcg gagctgccgc gggtggcggc cctcgtcagc
     4681 gcctttgcgg gcgtcaatca caactaccgg cgggagcacc gcttcaacct gtggttcgtc
     4741 gcggcggcgg cggacgagcg ggccctgaac ggcctgctgg ccgccatcgg cagcgccacc
     4801 ggttgtccgc ccgtggcgct gccgctggtg gaggagttcc acatcgatct gggcttcgat
     4861 ctggccaacg gccgccgcac tcgccacaag ccggcgccgg tgggcatgaa gcgtgcgctg
     4921 tcagccgccg aatggcgcct ggtcgaggcc ctgcaggacg gtctgccgtg ggtgccgcgg
     4981 ccctaccggg cggtggcaga cgcggccggt ctgaacgagg aggacgtcct cgccaccctc
     5041 gcggcgtggc tggccgaggg cacgctcagg cgcttcggcg tggtggtgcg ccatcgcgag
     5101 ctgggctggg acgccaacgc catgtgcgtg tgggccgtac cggaggccgc cgtgagcgct
     5161 gccggccggg ccctggccgc ccaggacggg gtgagcctgt gctaccgccg ccgaaccgcg
     5221 ccggagtggc cctactccct cttctgtatg ctccacgggc gttcccgcgc cgaggtggaa
     5281 gcgcgccgca tgaccgtcgc ggacgccgcc ggcgtcgcgg gctatccgtc cgcagtgctg
     5341 ttctccaccg actgttataa gcagtgcggc gcccgctacc gggatgccac accgcagccg
     5401 gcctgcgttt catgaaggcg gcggcgaccc tcgacgccct ggaccgggcc ctgatcaacg
     5461 cgctgcaagg gggtttcccc ctgtgtgacg cgccctacca ggcggtggcg gagcagctcg
     5521 gcaccaccga
//
LOCUS       DAOQPA010000171         5526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8907_length_5526_cov_14.448181, whole genome shotgun sequence.
ACCESSION   DAOQPA010000171 DAOQPA010000000
VERSION     DAOQPA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8907_length_5526_cov_14.448181"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1432
                     /locus_tag="PKX01_15970"
     CDS             170..1432
                     /locus_tag="PKX01_15970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMZ77681.1"
                     /translation="MNHAQSPPDAQPGAAQAAFQLNLRHLRGLTAVHEHGSISAAAGA
                     VGLSQPALTQGILKLEKLMGEVLFERRPDGIVPTAAGELVVDRARACLAHLATGTRQV
                     GGTAFEPDRRLSMTQLRAFVALVRAGSISAAAADLGLSQPAVHRAVRELEEALGRKLV
                     ERRGRGVHVNFAGRRFARSCRLALAELQAAFSELGRDPHNPTIALGTTPLARGFLVPE
                     AMALMVAERFPAGFRVHEGSWGELVEALRDGVIDIIVGEIPDDAAPDLATHPLYEEAP
                     VVVAGRQHPLATRRSCTPQMLASYPWIIAPENSPLRAEWERLFADRRPEAPVECGSIM
                     IIGRLLTASDLLTLAMPDQVALQVRTGLLSCIGAPLAGSRPTVGATMRKSWRLTRAQQ
                     RFLELLTVASAGIGADASRQSLIEPYWT"
     gene            1570..2859
                     /locus_tag="PKX01_15975"
     CDS             1570..2859
                     /locus_tag="PKX01_15975"
                     /inference="COORDINATES: protein motif:HMM:NF039910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMZ77682.1"
                     /translation="MLNSQDNETLVRVGPGTAMGQLMRLYWIPFLASADVERDGQPHR
                     VRLLGEDLVAFRDSEGQVGLVDQACPHRGAPMVFARNEEGGIRCIYHGWKFDVTGQCQ
                     ETPAEPPGSAMTSRMTIKSYPVRERNGVLWTYMGPKRETPPPLPELEWNMVPESHSVV
                     TVRVQECNWLQALEGEIDSAHAAILHGRNASTIAQWRQGQDLSPKFEVQPHDAGISIA
                     ARRKDGTEHNYVRVNQFLMPFWTLVPPQMQYPELSGHAWVPIDDHHTLAFTFSYTPDQ
                     PFYEKSRKLFKDGYKGRETGHHSHGAYEARPVTVPYHRYWSRFNRGNAYNYDYQTGMP
                     GLWLEDAACQSGVATICDRSQEHLGSTDAGIVRVRRLLLESAKKLAEGAEPVSAAKPS
                     SFMMRAISVTIPAGSDWMELGRDYMKAELGKGFGYTP"
     gene            2892..4211
                     /locus_tag="PKX01_15980"
     CDS             2892..4211
                     /locus_tag="PKX01_15980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HMZ77683.1"
                     /translation="MLENLEADFPEHKRRASGAPHALDGIRVVDFSHFIAGPLATMIL
                     ADMGAEVIKVEAPVRGDDARRYPPMHPQLDQGAPFVWSNRNKKSIALDLKSPQGLALA
                     RDLVCRADVLVENFSSGVMRRLGLDHETCLALNPRLVYCSVAAYSREGAFADRFGFDP
                     IAQAESGFVSMNGYPDREGVRALSPVMDISTAMMVGNAVLGALLARERLGRGQRVEVS
                     LFDTAVLMTGYATMQSLFTGKEPQRSGNTAPDTCPSGAFRASDRTFYINSGNDRIFQR
                     LMGQVLERPDVAADADYATGPLRIRHRERLFAFLDEAFARHDWAHWQARMRAAGVPYG
                     LVRTVGEAIRSPEARDHELVTRIPHPVLGWVPNVRLPIRYAATPMADPVAAPAVGQHT
                     QEVLRDALGLDERQIAELERAGAFGPAAAAAAAAVPGPMDRDPVPAS"
     gene            4267..5253
                     /locus_tag="PKX01_15985"
     CDS             4267..5253
                     /locus_tag="PKX01_15985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HMZ77684.1"
                     /translation="MTLHTRRSALRMLAAGGAAASVGLPAFSQQGEAFPSRVIRIVVP
                     YPAGGITDQLARAIAQPMREVLGQTVIVENKPGAGGTIGTDYVAKQPADGYTLVFGNS
                     GPSATAGLMRKLPYDPQKDFRPISGVMRVPMILAVPADSPHKSVAEFVAWARSRGDKV
                     NYGSTGVGGASHLYSEYFNELAGTRFQHIPYTGAPPLVTAFAGGQVQMAFVTGLDGAA
                     MVQAGKIRYLAVASPKRTPVLPDLPAIAEAVPGFQAVGWFGVLAPAGVPDPVAQKLSS
                     TIAAVVRRPEVSKFLTDRNVEPWGSTQEELGRTIEGEIAQWGPLVKKFNITV"
     gene            5267..>5526
                     /locus_tag="PKX01_15990"
     CDS             5267..>5526
                     /locus_tag="PKX01_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HMZ77685.1"
                     /translation="MAQRTLKGRVAIVGIGETHYYKHGQSPDAEFKLALQAVLRACAD
                     AGLDPRHIDGFASYGDDRSEATRLASALGLPRLRSATMQWGGG"
BASE COUNT          873 a         1961 c         1878 g          814 t
ORIGIN      
        1 aaacgcagcc cggaaattac gcgcaaatgc gtctgctgac ccgcggaaag taagtaccgg
       61 cgcctcagcg ccaagggtgg cggcaccggc ggtcaattca acaagcggaa gccccgacga
      121 gggtgcgctt tatagtggtg cctggccagc ttcctcttcc ttggcgacca tgaaccacgc
      181 gcagtctccg ccggacgcgc agcccggcgc ggcacaggcg gccttccagc tgaacctgcg
      241 ccacttgcgc ggcctgacgg ccgtgcacga gcacggcagc atcagtgcgg ccgctggcgc
      301 ggtggggctc agccagccgg cgctgacgca gggcatcctg aagctggaga agctgatggg
      361 agaggtgctg ttcgagcggc gcccggacgg gatcgtgccc acggcggccg gggaactggt
      421 ggtggaccgg gcgcgggcct gcctggccca cctggcaacg ggcacccgcc aggtgggcgg
      481 cacggcgttc gaaccggatc gccggctcag catgacgcaa ctgcgcgcct tcgtggcgct
      541 ggtgcgggcc ggcagcatct ccgctgcagc cgcagacctt gggctgtctc agccggcagt
      601 gcaccgggcc gtgcgcgaac tcgaggaggc gctgggacgc aagctggtgg agcgccgcgg
      661 ccgcggagtg cacgtcaact ttgccggtcg gcgttttgcc cgcagttgca ggctcgcgct
      721 ggccgaactc caggcggcgt tctccgagct cgggcgcgac ccgcacaacc ccaccatcgc
      781 tctcggcacg acccccctgg cacgtggctt cctcgtgccc gaggccatgg cgctgatggt
      841 ggcggaacgg tttcccgcgg ggttccgcgt gcacgaaggc agctggggcg aactggtgga
      901 ggcgctcagg gacggtgtga tcgacatcat cgtcggcgag atccccgacg acgcagcgcc
      961 ggacctggcc acgcacccgc tttacgagga agccccggtg gtggtggcgg ggcggcagca
     1021 tccgctcgcg acgcggcgca gttgcacgcc gcagatgctg gcttcctacc cctggatcat
     1081 cgcgccggag aactcgccgc tgcgtgccga atgggagcgc ctcttcgccg atcgccgccc
     1141 cgaggcgccg gtggagtgcg gctcgatcat gatcatcggc cgcctgctga ccgccagcga
     1201 cctgctgacg ctcgccatgc cggaccaggt ggcgctgcag gtgcgcaccg gcctgctcag
     1261 ctgcatcggc gcaccgctgg cgggaagcag gccgacggtc ggcgccacga tgcgcaagag
     1321 ctggcgcctc acgcgggccc agcagcggtt cctcgaactg ctgacggtgg cttccgccgg
     1381 cattggcgcc gacgcgagcc ggcagtcgct catcgagccc tactggacct gaaggccgct
     1441 gccaccactg tggggccagc tcaattccgg tttctcaata ccgcggcggc ctttcgaatc
     1501 acgcgccggc cggcactgcc tagaatcggg cgaatcgatg ttcggttcaa ccactcaccc
     1561 taaaaggcca tgctgaattc ccaagacaac gagacgctcg ttcgcgtcgg ccccggtacc
     1621 gcgatgggcc agctcatgcg gctgtactgg atccccttcc tcgcgtccgc cgatgtcgag
     1681 cgcgacggcc agccgcatcg ggtgcgcctg ctgggcgagg acctggtcgc gttccgcgac
     1741 agcgagggcc aggtcggcct ggtggaccag gcctgcccgc accgcggcgc gcccatggtg
     1801 ttcgcccgca acgaggaggg cggcatccgc tgcatctacc acggctggaa gttcgacgtg
     1861 acggggcagt gccaggagac cccggcggaa cccccgggca gtgccatgac cagccgcatg
     1921 accatcaagt cctatcccgt gcgcgagcgc aacggcgtgc tgtggaccta catgggcccg
     1981 aagcgcgaga cgccgccgcc gctgcctgag ctcgaatgga acatggtgcc cgagtcgcac
     2041 tccgtcgtca ccgtccgggt gcaggagtgc aactggctgc aggcactgga aggtgaaatc
     2101 gactccgcgc atgccgccat cctgcatggc cgcaacgcca gcacgatcgc gcagtggcgg
     2161 cagggccagg acctgtcgcc caagttcgag gtccagccgc acgatgccgg catcagcatc
     2221 gcggcgcggc gcaaggacgg caccgagcac aactacgtgc gggtcaacca gttcctgatg
     2281 ccgttctgga ccttggtgcc gccgcagatg cagtaccccg agctgagcgg ccatgcctgg
     2341 gtgcccatcg acgaccacca caccctggca ttcacgttct cgtacacgcc ggaccagccc
     2401 ttctacgaga agtcgcgcaa gctgttcaag gacggctaca agggccgcga gaccggccac
     2461 cattcgcatg gcgcctatga ggccaggccg gtgacggtgc cttatcaccg gtactggagc
     2521 cggttcaacc ggggcaacgc ctacaactac gactaccaga ccggcatgcc cggcctgtgg
     2581 ctggaggatg ccgcgtgcca gtcgggcgtg gcgaccatct gcgaccgttc gcaggagcac
     2641 ctgggctcca ccgatgcagg catcgtgcgc gtgcgccggc tgctcctgga gagcgcgaag
     2701 aagctggcgg agggcgccga gccggtctcc gccgcgaagc cgtcttcctt catgatgcgg
     2761 gccatctccg tcaccatccc cgccggttcc gactggatgg aactgggacg cgactacatg
     2821 aaggcggagc tcggcaaggg cttcgggtac acgccgtgag cggttctccg atgcaggcag
     2881 gcggccagga catgttggag aacctcgaag ccgactttcc ggagcacaag cgacgggcct
     2941 ccggcgcacc ccatgcgctg gacggcatcc gggtggtgga cttctcgcac ttcatcgcgg
     3001 ggccgctggc gaccatgatc ctggccgaca tgggcgccga ggtgatcaag gtcgaggcgc
     3061 cggtccgcgg cgacgacgcg cgccgctatc cgcccatgca cccgcaactg gaccagggcg
     3121 cacccttcgt ctggagcaac cgcaacaaga agagcatcgc gctggacctg aagtccccgc
     3181 aggggctggc gctggcgcgc gacctcgtct gccgcgccga cgtgctggtc gagaacttct
     3241 cctcgggcgt gatgcgtcgc ctggggctgg accacgagac ctgccttgcc ctgaacccgc
     3301 gcctggtcta ctgctcggtg gccgcctaca gccgcgaagg cgccttcgcc gaccgcttcg
     3361 gcttcgatcc catcgcgcag gccgaaagcg gcttcgtttc catgaacggt tatcccgacc
     3421 gcgaaggcgt gcgggcgctc tcgccggtga tggacatcag caccgccatg atggtgggca
     3481 acgccgtgct cggcgcgttg ctggcccggg agcgcctggg ccgcggccag cgggtggagg
     3541 tgtccctgtt cgacacggcc gtcctgatga cgggctatgc gaccatgcag tcgctgttca
     3601 ccggcaagga gccgcaacgc agcggcaaca ccgcccccga cacctgcccc tcgggcgcct
     3661 tccgcgccag cgaccgcacc ttctacatca acagcggcaa cgacagaatc ttccagcggc
     3721 tcatggggca ggtgctggaa cgtccggacg tggccgccga tgcggattac gccacgggtc
     3781 ccctgcgcat ccggcatcgg gagcgcctgt tcgccttcct cgacgaagcg ttcgcgcggc
     3841 acgactgggc gcactggcag gctcgcatgc gcgccgccgg cgtgccctac ggattggtgc
     3901 gcacggtggg cgaggcgatc cgctcgcccg aggcgcgcga ccacgaactc gtcacccgca
     3961 tcccccatcc ggtgctcggc tgggttccga acgtgcgctt gccgatccgc tacgccgcca
     4021 cgcccatggc cgatccggtg gcggccccgg cggtggggca gcatacgcag gaggtcctgc
     4081 gggacgctct tggcctggac gaacggcaga tcgccgaact ggagcgcgcc ggtgccttcg
     4141 gtcctgcagc cgcagccgca gccgcagccg tgccgggccc gatggaccgc gacccggtgc
     4201 ccgcatcttg atttcaaccc cgcgcgaccg gcagcagcgg cgcgcattca caaccaggag
     4261 acaaccatga ccctgcacac ccgtcgcagc gcactcagga tgctggccgc cggcggcgcc
     4321 gccgcttccg tggggcttcc cgccttcagc cagcaggggg aggcgtttcc ctcccgggtg
     4381 atccgcatcg tcgtgccgta cccggccggc ggtatcaccg accagctcgc acgggcgatc
     4441 gcccaaccga tgcgcgaggt gctgggccag accgtgatcg tcgaaaacaa gcctggagcc
     4501 ggcggcacca tcggtaccga ctacgtcgcc aagcagcccg cggacggcta cacgctggtg
     4561 ttcggcaaca gcggcccgag cgccaccgcc ggcctgatgc gcaagctgcc ctacgacccc
     4621 cagaaggact tccggccgat ctccggcgtg atgcgggtcc cgatgatcct cgccgtgccc
     4681 gccgattcgc cccacaagtc cgtggccgaa ttcgtcgcgt gggcgcggtc gcggggcgac
     4741 aaggtgaatt acggctccac cggcgtcggc ggcgcttccc acctctacag cgagtacttc
     4801 aacgagctgg ccggcacccg cttccagcac atcccttata ccggcgcccc gccgctggtg
     4861 accgctttcg ccgggggcca ggtgcagatg gcgttcgtga cggggctgga tggcgctgcc
     4921 atggtgcagg ccggcaagat ccgctacctg gcggtggcgt cgcccaagcg gacgccagtg
     4981 ctgccggacc tgcctgccat cgccgaggcc gtgcccggat tccaggccgt ggggtggttc
     5041 ggcgtgctgg ccccggctgg cgtgcccgac cccgtcgcac agaagctttc cagcaccatc
     5101 gccgccgtgg tgcgccgccc cgaggtcagc aagttcttaa ccgaccgcaa cgtggagccg
     5161 tggggcagca cgcaggaaga gctgggccgc acgatcgaag gcgagatcgc ccagtggggg
     5221 ccgctcgtga agaagttcaa catcactgtc tgatgcggtc ccaacgatgg cccagcgcac
     5281 actgaagggc cgtgtcgcga tcgtcggcat cggggagacg cactactaca agcacggcca
     5341 gtctcccgat gccgagttca agctggcgct gcaggccgtg ctgcgcgcct gcgccgacgc
     5401 gggcctggat ccacgccaca tcgacggctt cgcctcgtac ggcgacgacc gcagcgaagc
     5461 cacccgcctg gcctccgcgc tggggctgcc gcgcctgcgc tccgccacca tgcagtgggg
     5521 cggcgg
//
LOCUS       DAOQPA010000172         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_8936_length_5509_cov_21.426843, whole genome shotgun sequence.
ACCESSION   DAOQPA010000172 DAOQPA010000000
VERSION     DAOQPA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8936_length_5509_cov_21.426843"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1194)
                     /locus_tag="PKX01_15995"
     CDS             complement(70..1194)
                     /locus_tag="PKX01_15995"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HMZ77686.1"
                     /translation="MQAHSVGIVAPQRARFTEALPLQSGGVLPEYDLVYETYGQLNAA
                     RSNAVLVCHALSGSHHVAGTYADQPGSEGWWDNLVGPGKPLDTNRFFVIGVNNLGGCY
                     GSSGPNALNPATGKPWGADFPFVTVEDWVNAQARLADRLGIERFAAVVGGSLGGMQAL
                     SWTLQYPGRVAHALVIASAPKLTAQNIAFNEVARQAILKDPDFHGGHYYEHGVVPARG
                     LALARMVGHITYLSDDAMGQKFGRTLRHDKPVFSYDVEFEIESYLRYQGDKFAGYFDA
                     NTYLLTTKALDYYDPAFAHQGNLVAALARAKADYLVVSFTTDWRFSPARSREIVYALV
                     HSDRRVSYAEIDCPAGHDSFLLDDPQYHAVLRAYIDNIRV"
     gene            complement(1367..3088)
                     /gene="ptsP"
                     /locus_tag="PKX01_16000"
     CDS             complement(1367..3088)
                     /gene="ptsP"
                     /locus_tag="PKX01_16000"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HMZ77687.1"
                     /translation="MSFTVHGLAVSGGIAIGHAHLVSHALLEVAHFTVAPRHVEAEVA
                     RLHGAVATVKADLAELKAATGAGAAPSEVDAFVGMHIMFLEDPMLVDAAEALIRTRRA
                     NAEWALVQQMEHLVEQFEAIEDAYLRERKADVVQVVERIVKVLLGHPGHLPPKRKDGL
                     GTIVVAHDLSPTDTIGFKDHAVAGFITDVGGPTGHTAIVARSLSIPAVVALRHIRHVV
                     KDDEMIIIDGTRGVVIVDPDERVLEEFRLRKVEFELERSKLKRLKSTRAATLDGEEVQ
                     LLANIELPPDAAQAKSVEADGVGLFRTEFLFMSRETWPDEDEQFEAYRSVVKTMSGKP
                     VTVRTLDVGADKELEGATRAEDNPALGLRAIRYCLSEPKMFLTQLRALLRASHYGRLK
                     ILIPMLASSHEIDQTLVLLEKAKEQLRAKKIKFDEAVPVGGMIEVPAAALCLGAFTRK
                     LQFLSIGTNDLIQYTLAIDRGNEAVAHLYNPLHPAVLRLLYQTIQAGAKAGVPVSVCG
                     EMAGDPSCARLLIGMGLRHFSMHPAQILEVKREILRADAADLTAKVQRLLKLDEPDRV
                     REAVERL"
     gene            complement(3147..3416)
                     /locus_tag="PKX01_16005"
     CDS             complement(3147..3416)
                     /locus_tag="PKX01_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HMZ77688.1"
                     /translation="MPRQEAEIINKLGLHARASAKLTQTASNYASEVWLERNGRRVNA
                     KSIMGVMMLAAAKGSTIVVETEGTDADQALSAVIALIADKFGEEE"
     gene            complement(3482..3865)
                     /locus_tag="PKX01_16010"
     CDS             complement(3482..3865)
                     /locus_tag="PKX01_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HMZ77689.1"
                     /translation="MIGLLLITHGSLGEALIQCVSHVLNRRPPQLMQIGVTGQDDPTE
                     SLPLARRLLHMVDSGDGVLILTDVLGATPSNMATKLLEPGRIEGIAGVNLPMVLRCLT
                     YRERNMETLVKKAVAGACEGVVRIR"
     gene            complement(3949..5010)
                     /locus_tag="PKX01_16015"
     CDS             complement(3949..5010)
                     /locus_tag="PKX01_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HMZ77690.1"
                     /translation="MSPLRPGRRSLLRALLGLCALALLAGCQRERIQQQESYVFGTRV
                     EVLTWDADETRARDAVAEVLREFDRLHRAYHAWEPSELTALNAAIAAGKQAIPVSPEL
                     AAMLSDAQAIAATGDQLFDPALGQLIALWGFHTDTFVPHRPAPAALAAAVRAHPQMAD
                     LTIQNNTVSSRNPAVQLDLGGYGKGYALDRAADILRARGVRNALINIGGNVMALGSKG
                     EQPWRVGIQHPREPRPLASLPLRDGEAIGTSGDYQRFFELDGQRYCHLIDPRTGLPAR
                     GTQAVTILITPRAHAGTLSDAASKPIYLGGERWREYAARFGIDHALRVDADGHIQVTR
                     ALERRLQFSDGIKPSTVVD"
     gene            complement(5007..>5509)
                     /locus_tag="PKX01_16020"
     CDS             complement(5007..>5509)
                     /locus_tag="PKX01_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HMZ77691.1"
                     /translation="AFIDAHGDTILKPLDGMGGSQIFRVRADDPNRNVIVETLTHGET
                     RSIMAQAYLPAIVHGDKRVLIVGGRVIPYSLARIPKDGETRGNLAAGGRGVAMPLTDG
                     ERAIAGHLAPILWARGLLIVGLDVIGDRLTEINVTSPTCMVEITAQTGFDVPGAVIDA
                     LGAACA"
BASE COUNT          792 a         1645 c         2126 g          946 t
ORIGIN      
        1 acccgctcgc cgggttcgat ccagttggcg atcacgtcgt agtcgtagcg cagactcatg
       61 atggtggcct cacacccgga tgttgtcgat gtaggcccgc agcacggcgt ggtactgggg
      121 gtcgtcgagg aggaaggagt cgtggccggc gggacagtcg atctcggcgt agcttacccg
      181 ccggtcgctg tgcaccagcg cgtagacgat ctcccgactg cgggccgggg agaagcgcca
      241 gtcggtggtg aaggacacga ccaggtagtc ggccttcgcc cgcgccaggg ccgccaccag
      301 attgccctgg tgggcgaagg ccgggtcgta gtagtcgagg gccttggtgg tgagcaggta
      361 ggtattggcg tcgaaatagc cggcgaactt gtcgccctgg tagcgcaggt aggactcgat
      421 ctcgaattcc acgtcgtagc tgaacaccgg cttgtcatgg cgcagggtgc ggccgaattt
      481 ctggcccatg gcgtcgtccg acaggtaggt gatgtgaccc accatgcgcg ccagggcgag
      541 tccgcgggcg ggcaccacgc cgtgctcgta gtagtggccg ccgtggaagt ccgggtcctt
      601 gaggatggcc tgacgggcga cctcgttgaa ggcgatgttc tgggcggtga gcttgggggc
      661 ggaggcgatc accagggcgt gggcgacccg cccggggtat tgcagggtcc acgacagggc
      721 ctgcatgccg cccaggctgc cgcccaccac cgcggcgaag cgctcgatgc cgaggcggtc
      781 ggccaggcgc gcctgggcgt tgacccagtc ctccacggta acgaagggga aatcggcgcc
      841 ccagggcttg ccggtggccg ggttgagggc gttggggccg gaactgccgt agcagccgcc
      901 caggttgttg acgccgatga cgaagaagcg gttcgtgtcg aggggcttgc ccggcccgac
      961 caggttgtcc caccagcctt cggagccggg ctggtcggca taggtgccgg ccacgtggtg
     1021 ggagccggac agggcgtgac agaccagcac ggcgttgctg cgcgcggcgt tgagctgacc
     1081 gtaggtctcg tagaccaggt cgtattccgg cagcacgccg ccgctctgga gggggagggc
     1141 ttcggtgaag cgggcgcgct ggggcgccac gattccgacg gagtgtgctt gcatccttgt
     1201 tgtcccgaaa aacagaaacc cagccggcga acggtcgcgg actgggctgc cgtcgcttta
     1261 gccgtacttg taaagcgccc gcaagctgaa gggttcaaat cggcgctgga tcgaagagta
     1321 gcgcgtcgtc ggcggccgcg tcaattgctg cgcccgccga cgccgttcac aagcgctcca
     1381 ccgcttcgcg cacccggtct ggttcgtcga gcttgagcag gcgctgcacc ttggcggtca
     1441 ggtcggccgc gtcggcgcgc aggatctcgc gcttgacctc caggatctgg gccggatgca
     1501 tcgagaagtg gcgcaggccc atgccgatga gcaggcgcgc gcaactggga tcacccgcca
     1561 tttcgccaca caccgaaacc ggcacgccgg ccttggcacc ggcctggatg gtctggtaga
     1621 gcagacgcag caccgccgga tggagcgggt tgtagaggtg ggcgacggcc tcgttgcccc
     1681 ggtcgatggc cagggtgtac tggatcaggt cgttggtgcc gatggacagg aactgcagct
     1741 tgcgggtaaa ggcgccgagg cacagggccg ccgcgggcac ctcgatcatg ccgccgaccg
     1801 ggaccgcttc gtcgaacttg atcttcttgg cgcgcaactg ctccttggcc ttttcgagga
     1861 ggaccagggt ctggtcgatc tcgtggctgc tggccagcat ggggatcagg atcttgagcc
     1921 tgccgtagtg gctggcccgg agcagtgccc gcagttgggt gaggaacatc ttgggctcgg
     1981 acaggcagta gcggatcgcc cgcaggccca gggccgggtt gtcctcggcg cgggtggcgc
     2041 cttccagttc cttgtcggcg cccacgtcca gggtgcgtac cgtgaccggc ttgccggaca
     2101 tggtcttgac cacgctgcgg taggcctcga actgctcgtc ctcgtcaggc caggtttccc
     2161 ggctcatgaa caggaattcg gtgcggaaca ggccgacgcc gtccgcttcg acgctcttgg
     2221 cctgggcggc atcgggcggc agctcgatgt tggcgaggag ctgcacctct tctccgtcca
     2281 gggtggcggc gcgggtggac ttgaggcgct tgagcttgga gcgttcgagt tcgaattcca
     2341 ccttgcgcag gcggaactct tcgagcacgc gctcgtccgg atcaacgatg acgaccccgc
     2401 gggtgccgtc gatgatgatc atctcgtcgt ccttgaccac gtggcggatg tggcgcagcg
     2461 cgaccacggc cgggatggac aggctgcggg cgacgatggc agtgtgcccg gtgggtccgc
     2521 ccacgtcggt gatgaaaccg gcgacggcgt ggtccttgaa gccgatggtg tcggtgggcg
     2581 acaggtcgtg ggcgacgacg atggtgccga ggccgtcctt gcgcttgggc ggcaggtggc
     2641 ccggatggcc gaggagcacc ttgacgatgc gttccaccac ctgcaccacg tcggccttac
     2701 gctcccgcag gtaggcgtcc tcgatggcct cgaactgttc gaccagatgc tccatctgct
     2761 gcaccagggc ccactcggcg ttggcgcggc gggtgcggat gagcgcctcc gccgcgtcga
     2821 cgagcatggg gtcttcgagg aacatgatgt gcatgccgac gaaggcatcc acctcgctgg
     2881 gggcagcgcc cgcaccggtg gcggccttga gctcggccag gtcggccttg acggtggcga
     2941 cggcgccatg caggcgcgcg acttccgcct ccacgtggcg cggtgccacg gtgaagtggg
     3001 cgacctccag cagggcgtgg gagaccaggt gggcgtggcc gatggcgatg ccgcccgaga
     3061 cggcgaggcc gtggacggtg aagctcatgg gcggtcctcc ggagcggggt gccggggagc
     3121 ggtgccggcg gcgaggccgg cggcgttcat tcctcttccc cgaacttgtc ggcgatcagg
     3181 gcgatcaccg cgctgagggc ctggtctgcg tccgtgcctt cggtttccac gacgatggtg
     3241 gagcccttgg cggcggccag catcatgacg cccatgatgc tcttggcgtt gacgcgacgg
     3301 ccgttgcgtt cgagccagac ttcgctggcg tagttgctgg cggtctgggt gagcttggca
     3361 gaggcgcggg catggagccc gagcttgttg atgatttcgg cttcctggcg tggcatggag
     3421 ggtgttctcc cgtttcctcg aagtgtggtg tacggattgt tgttattgac gtgatgacgg
     3481 cctatcggat gcgcacgacg ccttcgcagg cgccggccac cgctttcttg accagggttt
     3541 ccatgttgcg ttcccggtag gtgaggcagc gcagcaccat gggcaggttg acccccgcga
     3601 tgccttcgat gcggcccggt tccaacagtt tggtggccat gttggagggg gtggcgccga
     3661 ggacgtcggt gaggatcagc accccgtcgc cgctgtccac catgtggagc aggcggcggg
     3721 ccaggggcag ggattcggtc gggtcgtcct ggccagtgac gccgatctgc atcaactggg
     3781 gcggtcgccg gttgaggaca tgactgacgc actggatcag ggcctcgccg agactgccgt
     3841 gggtgatgag cagaaggccg atcatgtccc gattctatcc gaaggccgga gcggggcaag
     3901 ccgggaatgt aagggggaat gagcccgtct tttcggaccg acccggtgtc agtccacgac
     3961 cgtcgagggc ttgatgccgt ccgagaactg caggcgccgt tcgagggcgc gggtgacctg
     4021 gatgtggccg tcggcatcca cccgcagggc gtggtcgatg ccgaagcggg cggcgtattc
     4081 gcgccagcgc tcgccgccca ggtagatggg cttgctggcg gcgtcggaga gggtgccggc
     4141 gtgggcgcgc ggagtgatca ggatggtcac cgcctgggtg ccgcgggcgg gcaggccggt
     4201 gcgcgggtcg atgagatggc agtaacgctg gccgtccagc tcgaaaaaac gctggtagtc
     4261 gcccgaggtg ccgatggcct cgccgtcgcg caggggcagg gaggcgaggg ggcgcggttc
     4321 acgggggtgc tggatgccga cccgccaggg ttgctcgcct ttgctgccga gggccatgac
     4381 gttgccgccg atgttgatga gggcgttgcg gacaccgcgg gcgcgcagga tgtcggcggc
     4441 ccggtcgagg gcgtaaccct tgccgtagcc gcccaggtcg agctgcacgg ccgggttgcg
     4501 gctgctcacc gtgttgttct ggatcgtcag gtcggccatt tgcgggtggg ccctgactgc
     4561 ggcggccagg gctgccggag cgggccggtg cggcacgaag gtgtcggtgt ggaaacccca
     4621 cagggcgatg agctggccga gggccgggtc gaagagctgg tcgccggtgg cggcgatggc
     4681 ctgggcgtcg ctgagcatcg cggcgagttc cggcgacacc ggaatggcct gtttgccggc
     4741 ggcgatggca gcgttgaggg cagtgagctc ggagggttcc caggcgtgat aggcccggtg
     4801 caggcggtcg aattcgcgca gcacctcggc caccgcgtcg cgggcgcggg tctcgtcggc
     4861 gtcccaggta aggacttcga cgcgggtgcc gaagacgtag gattcctgct gctggatgcg
     4921 ttcccgctgg cagccggcga gcagcgccag ggcgcacagg ccgaggagtg cgcgcaggag
     4981 gctgcgccgg ccgggccgca gggggctcat gcgcaggcgg ctcccagggc gtcgatgacg
     5041 gcgccgggca cgtcgaagcc ggtctgggcg gtgatctcca ccatgcaggt ggggctggtg
     5101 acgttgatct cggtgaggcg gtcgccgatc acgtccaggc caacgatgag caggccgcgg
     5161 gcccacagga tcggcgccag gtgcccggcg atggcccgct cgccgtcggt gaggggcatg
     5221 gccacgccgc ggccgccggc ggcgaggttg ccgcgggtct cgccgtcctt ggggatgcgg
     5281 gcgaggctgt aggggatgac ccggccgccg acgatcagca cgcgcttgtc gccgtggacg
     5341 atggccggca ggtaggcctg agccatgatg ctgcgggtct cgccgtgggt gagggtctcg
     5401 acgatgacgt tgcggttggg gtcgtcggcg cggacgcgaa agatctggct gccgcccatg
     5461 ccgtcgaggg gcttgaggat ggtgtcgccg tgggcgtcga tgaaggcgt
//
LOCUS       DAOQPA010000173         5278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_9534_length_5278_cov_14.997894, whole genome shotgun sequence.
ACCESSION   DAOQPA010000173 DAOQPA010000000
VERSION     DAOQPA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9534_length_5278_cov_14.997894"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKX01_16025"
     CDS             <1..638
                     /locus_tag="PKX01_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77692.1"
                     /translation="KPAPTQTARAERRPPVLTAQAPSSARALATSPTWSITQKQEMDR
                     FLDDLATDARRAPSLAERSLAMAREIGREQARRNDDAYDLVERRPNSPEIDPLSLEMY
                     MESLIRKLNKAAGFVRNDPRTRGVQTASVLVRIAPDGSLESFKVLRVADQQSEIEFTH
                     AVVRQASPFAAFPPDIQRSARSLAMLICIRPPSLSGGAFGFTRMTEGRGCG"
     gene            670..1224
                     /locus_tag="PKX01_16030"
     CDS             670..1224
                     /locus_tag="PKX01_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77693.1"
                     /translation="MPPMSRRPPRGGRLVLPALTALLAACAGFGPAPDAATGPRTRDA
                     LVMHFGPPALRWPESDGGERLAFPTGPMGVHTWMARVDTTGRVLTVENVLVPARFAEI
                     QPGMGQDEVLRTLGPPQPHWTVYYKARDELVWEWRFCDDFQEPARFDVLFDGTSGRVR
                     STLAQPERLSNPWGRGGRRDWCSR"
     gene            1259..2755
                     /gene="glpK"
                     /locus_tag="PKX01_16035"
     CDS             1259..2755
                     /gene="glpK"
                     /locus_tag="PKX01_16035"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMZ77694.1"
                     /translation="MTFLLALDQGTTSSRAIVFDAAGQAVASAQQEFRQHYPQPGWVE
                     HDADEIWTTQRDCVRLALARAGLAAADIAALGIANQRETTVLWERASGRPLAPAIVWQ
                     DRRTAGHCDRLRAAGHADLIRARSGLELDAYFSATKLAWLLDHVPDARRRAEAGELAF
                     GTLDSWLVWQLTGGTRHVTDPSNAARTMLFNLHSLAWDDTLLDLFAIPRTLLPEVVDT
                     AGVLGATRPDLFGAPIPIAALAGDQQAATFGQACFAPGSAKNTYGTGCFLMLNTGATP
                     VASTQRLLSTVGWRLPGETCYMLEGSVFIGGAVIQWLRDGLGLIEHAADVEALAASVP
                     DSGGALLVPAFAGLGAPWWDGHARGTLVGLTRGVTRAHIARAALDAIALQTVDLVDAM
                     NRDGAGPLRELRVDGGAARNDLLMQIQADLLGVPVIRPRQTETTALGAACLAGLAAGV
                     WRSPAELARHWQEERRFEPTRSADWRAARLHDWHRAVARSRAWAEDAP"
     gene            2752..4272
                     /locus_tag="PKX01_16040"
     CDS             2752..4272
                     /locus_tag="PKX01_16040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HMZ77695.1"
                     /translation="MKRADRLIRLRHTARWDILIVGGGATGLGCAVDAAARGHSTLLL
                     EAGDFAGGTSSRSTKLIHGGVRYLAQGRLGLVREALAERTRLLRNAPHLVHPQGFVVP
                     VHGAWEGLRLGLGLRLYDHLAGADGLGPCRSLSADATLAALPGLRPDGLRGGLRYQDA
                     RFDDARLAITLMRSAEALGALTLNALRVTGLLHAGGRVAGVAAEDGETGEHFELPARV
                     VINATGVWSDAVRRLDDPDAPPQLAPSQGAHVVVDRDFLPGDDALLIPRTDDGRVLFV
                     IPWQGRLLLGTTDTPRTDLPAEPRPLDAEIDFILTTAARYLTRAPRRDDVRSAFAGLR
                     PLIGGTGASARLSREHLIQVSTRGLVSVAGGKWTTYRSMAEAVVDRAEAVGGLPPRPC
                     PTPTLRLFGAPTAPTGDVYGCERAAIERLPGAGRRLHPAFTLSEAEVRHAARAEQARS
                     VADVLARRHRALFIDAAAAQQAAPAVAAVMASELGWDAPRTHEMTEAFHRLAEGFR"
     gene            complement(4306..>5278)
                     /locus_tag="PKX01_16045"
     CDS             complement(4306..>5278)
                     /locus_tag="PKX01_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMZ77696.1"
                     /translation="VDAAVFETARGGVLREGLAFDRCDVAVVTNIGMGDHLGLSYIST
                     VEDLAVVKRVIVQNVAPHGTAVLNAADPMVVRMASACPGSVLFFAADEHNPVLATHRV
                     QGHRVVFVDEDAIVAAQKDREFRIPLAQIPLTRNGTISFQVENAMASIAAAWALGIDP
                     ETIRAGVATFVNDAATAPGRFNVFDYKGATVIADYGHNPDAILALVQAVESIPAKRRS
                     VVISGAGDRRDEDLRRQTQILGDIFDEVVLYQDLCQRGRADGEVLALLREGLTQAKRA
                     TEVLEIRGEFLAIDTAFAKLQPGDLCLVLVDQVEEALEHIARRIAEA"
BASE COUNT          715 a         2154 c         1709 g          700 t
ORIGIN      
        1 ccaaacccgc gcccacccag accgcccgcg ccgagcgccg cccacccgtc ctcaccgccc
       61 aggccccgtc gtccgcccgc gcgctggcga catcccccac ctggagcatc acccagaagc
      121 aggagatgga ccgcttcctg gacgacctgg caaccgatgc ccgccgcgcc cccagcctcg
      181 ccgagcgctc cctggccatg gcgcgggaga tcggccgcga gcaggcccgc cgcaacgacg
      241 atgcctacga tctcgtcgag cgccggccca acagccccga aatcgacccc ctgagcctcg
      301 agatgtacat ggagtccctc atccgcaagc tcaacaaggc cgccggtttt gtccggaacg
      361 acccgcgcac ccgcggcgtg cagacggcgt cggtgctggt gcgcatcgcg ccggacggca
      421 gcctggaaag cttcaaggtg ctgcgcgtgg ccgaccagca gtccgagatc gaattcaccc
      481 acgccgtcgt ccgccaggcg tctcccttcg ccgcctttcc accggacatc cagcgctccg
      541 cgcgctccct ggccatgctg atctgcattc gtccgccgtc cctcagcggc ggtgccttcg
      601 gcttcacgcg gatgaccgag gggcgcggct gcggctagaa tgccgacatc gtcgcgcgga
      661 gccgtcacca tgcctccgat gtcgcgccgc ccgccccgcg gcggccgcct cgtcctgccc
      721 gccctcacgg ccctgctcgc cgcctgcgcc ggtttcggac ccgcgcccga cgccgccacc
      781 ggcccgcgca cccgcgatgc cctcgtgatg cacttcggcc cgccggcgtt acgctggccc
      841 gagtccgacg gcggcgagcg cctcgccttt cccaccggcc cgatgggcgt ccacacctgg
      901 atggcccgcg tcgacaccac cggccgcgtc ctcaccgtcg agaacgtcct ggtcccggcc
      961 cgcttcgccg agatccagcc cggcatgggc caggatgaag tcctgcgcac cctcggcccg
     1021 ccccagcccc actggaccgt ctactacaag gcccgcgacg agctcgtgtg ggaatggcgc
     1081 ttctgcgacg acttccagga gcccgcccgc ttcgacgtgc tcttcgacgg caccagcggc
     1141 cgggtacgca gcaccctggc ccagcccgag cgcctctcca atccctgggg acgcggtggc
     1201 cggcgcgact ggtgcagccg ctgaccgccc tcctcccgac cgcccgcgga gactgcccat
     1261 gaccttcctc ctcgccctcg accagggcac caccagctcc cgggccatcg tcttcgacgc
     1321 cgccggccag gccgtcgcca gcgcccagca ggaattccgc cagcactacc cgcagcccgg
     1381 ctgggtcgag cacgacgccg acgagatctg gaccacccag cgcgactgcg tccgcctggc
     1441 cctcgcccgg gccggtctcg ccgcggccga catcgcagcc ctgggcatcg ccaaccagcg
     1501 ggaaaccacc gtgctgtggg agcgggccag cggacggccc ctcgcccccg ccatcgtgtg
     1561 gcaggaccgc cgcaccgccg gccactgcga ccgcctgcgt gccgccggtc acgcagacct
     1621 gatccgcgcc cgcagcggtc tggagctgga cgcctacttc tctgccacca agctcgcctg
     1681 gctgctggac cacgtccccg acgcccggcg ccgcgccgaa gccggcgagc tggccttcgg
     1741 caccctcgac agctggctgg tgtggcagct caccggcggc acacgacacg tcaccgaccc
     1801 gagcaacgca gcccgcacga tgctcttcaa cctgcacagc ctggcttggg acgacacact
     1861 cctcgacctc ttcgcgatcc cccgcaccct gctgccggag gtggtggaca cggccggcgt
     1921 cctgggcgcc acgcggccgg atctgttcgg cgcccccatc cccatcgccg ccctggccgg
     1981 cgaccagcag gccgccacct tcgggcaggc ctgcttcgcg ccgggcagcg ccaagaacac
     2041 ctatggcacc ggctgcttcc tgatgctcaa caccggcgcc acgccggtcg cctccaccca
     2101 gcgcctgctc agcaccgtcg gctggcgcct gcccggcgag acctgctaca tgctggaagg
     2161 cagcgtcttc atcggcggcg cggtgatcca gtggctgcgc gacggcctcg gcctgatcga
     2221 gcacgccgcg gatgtggaag cccttgccgc cagcgtgccc gacagcggcg gcgccctcct
     2281 cgtcccggcc tttgcgggcc tcggcgcccc ctggtgggac ggccacgccc gcggcaccct
     2341 ggtcggcctg acccgcggtg tcacccgtgc ccacatcgcc cgcgccgcgc tggacgccat
     2401 cgccctacag accgtcgacc tggtggacgc catgaaccgg gacggcgccg gccccctccg
     2461 cgaactgcgc gtggacggcg gcgccgcccg caacgacctg ctcatgcaga tccaggccga
     2521 cctgctgggc gtccccgtga tccgcccgcg ccagaccgag accaccgccc tcggcgccgc
     2581 ctgcctggcc ggactggcgg cgggcgtgtg gcgttcgccg gcggagctcg cccggcactg
     2641 gcaggaggaa cgccgcttcg agcccacccg ctccgccgac tggcgcgccg cgcgcctcca
     2701 cgactggcac cgggccgtgg cgcgcagtcg cgcctgggcc gaagacgccc cgtgaagcgc
     2761 gccgaccgcc tgatccgcct gcgccacacg gcgcgctggg acatcctcat cgtcggcggc
     2821 ggcgccaccg gcctgggctg cgccgtggat gcggccgccc gcggccactc caccctgctg
     2881 ctggaagccg gcgactttgc cggcggcacc agttcccgct ccaccaagct gatccatggt
     2941 ggcgtgcgct acctggccca gggccgcctc gggctggtcc gcgaagccct cgccgaacgc
     3001 acccgcctgc tccgcaacgc gccccacctg gtccatcccc agggcttcgt ggtgccggtc
     3061 cacggtgcct gggagggtct gcgcctgggc ctcggcttgc gcctgtacga ccacctcgcc
     3121 ggcgcagacg gtctgggccc ctgccgcagc ctgtccgcgg acgccaccct cgccgccctg
     3181 cccggcctgc gtccggatgg cctgcgcggc ggcctccgct accaggacgc ccgcttcgac
     3241 gacgcgcgcc tggccatcac cctgatgcgc agcgccgaag ccctgggcgc cctcaccctc
     3301 aacgccctgc gcgtcaccgg cctgctccac gcgggcggtc gcgtcgccgg cgtggcagcc
     3361 gaggatggcg agaccggcga acacttcgag ctgcccgcgc gggtcgtcat caacgccacc
     3421 ggggtgtgga gcgatgccgt gcgacgcctc gacgacccgg acgcaccgcc gcagctggcc
     3481 cccagccagg gcgcccacgt ggtggtggac cgggatttcc tgcccggcga cgacgccctc
     3541 ctgatccccc gcaccgacga cggccgggtg ctcttcgtca tcccctggca gggccgtctg
     3601 ctcctcggca ccaccgacac accgcggacc gacctgccgg ccgagccccg cccgctggac
     3661 gccgagatcg acttcatcct caccaccgcc gcccgctacc tgacccgtgc gccgcggcgg
     3721 gacgacgtgc gcagcgcctt tgccggcctg cgccccctga tcggcggcac cggcgccagc
     3781 gcccgcctga gccgcgagca cctcatccag gtctcgaccc gcggcctcgt cagcgtggcg
     3841 ggcggaaaat ggaccactta ccggagcatg gccgaggcgg tggtggaccg cgccgaggcg
     3901 gttggcggcc tgcccccacg accctgcccg acccccacgc tacgcctctt cggcgcccca
     3961 accgcgccga ccggcgatgt gtacggctgc gagcgcgcag ccatcgagcg tctgcccggc
     4021 gcaggccggc gtctgcatcc tgccttcacc ctgagcgagg ccgaggtgcg ccatgcagcg
     4081 cgcgcagaac aggcccgcag cgtggccgac gtcctcgccc gccgccaccg ggccctgttc
     4141 atcgacgccg ccgccgccca gcaggccgcc cccgccgtgg cggcggtcat ggccagcgaa
     4201 ctgggctggg atgcaccgcg cacccatgaa atgacggaag ccttccaccg tctggcggaa
     4261 ggcttccggt aagcccacgc cggccccagg gccgggcctc gcgggtcagg cttcggcaat
     4321 gcgccgggcg atgtgctcca gcgcctcttc cacctgatcc accagcacca ggcacaggtc
     4381 gcccggctgg agcttggcga aggcggtgtc gatggccagg aattcgccgc ggatctcaag
     4441 gacctcggtg gcccgcttgg cctgggtcag cccttcccgc agcagagcca gcacctcgcc
     4501 gtcggcgcgg ccacgctggc acagatcctg gtacagcacc acctcatcaa agatgtcgcc
     4561 aaggatctgg gtctggcggc gcaggtcctc gtcgcggcgg tcgccggcgc cgctgatcac
     4621 caccgagcga cgcttggccg ggatgctctc gacggcctgc accagggcca ggatggcgtc
     4681 cgggttgtgg ccgtagtcgg cgatcacggt ggcgcccttg taatcgaaca cgttgaagcg
     4741 tccgggcgcc gtggcggcat cattcacgaa agtcgccacg ccggcgcgga tcgtctccgg
     4801 atcgatgccc agggcccacg ccgcggcgat ggaggccatg gcgttctcca cctggaaaga
     4861 gatggtgccg ttgcgggtga ggggaatctg cgccagcgga atgcggaatt cccggtcctt
     4921 ctgggcggcg acgatggcgt cctcatcaac aaagaccacc cggtgaccct gcacgcggtg
     4981 ggtggcgagc accgggttgt gctcgtcggc ggcaaagaac agcaccgagc cggggcaggc
     5041 gctggccatc ctgacgacca tcgggtcggc cgcgttgagc accgcggtgc cgtggggcgc
     5101 cacgttctgc acgatgaccc gcttgaccac cgccagatcc tcgacggtgc tgatgtagga
     5161 caggcccagg tggtcgccca tgccgatgtt ggtgaccacc gccacgtcgc agcggtcgaa
     5221 ggcgaggcct tcccgcagca cgccgccgcg ggcggtctcg aacaccgccg cgtccacg
//
LOCUS       DAOQPA010000174         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_9546_length_5274_cov_17.627132, whole genome shotgun sequence.
ACCESSION   DAOQPA010000174 DAOQPA010000000
VERSION     DAOQPA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9546_length_5274_cov_17.627132"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKX01_16050"
     CDS             <1..542
                     /locus_tag="PKX01_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77697.1"
                     /translation="RGPQAGGAAMSLQIGTVSIEALESLDLDQTYEILGGEAIQRTVG
                     GSGLKQMTWARLRVQTSGQGWLPPGLADLDTSQQMTLRCIVPRAIPCTGLVATLPAAR
                     RSDAGYLPYGIATLASGQAVKTSAVLVGNVATLAAVAGAVSYRALYYPELTVWAMRPS
                     HSGSRSEATHRWEITCEEV"
     gene            686..1456
                     /locus_tag="PKX01_16055"
     CDS             686..1456
                     /locus_tag="PKX01_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77698.1"
                     /translation="MIEWGSGASAGAEWGIGTLNAAGTLLTRTFVLGHTPSTLRAGPG
                     TTPVDLPGGTKTVSLAAIDTMAVAACPDLTQTTLPSPRADGVLAMAVGIGSSAVGDWS
                     CAFGPYASARHPSSVVVGPGLSIGPKSATLAAGDGLVAEQCFAGNAASGAPFVFDGPT
                     LALSSDAPYWIEMTAIACNAAMTQFRAIRRTVFAAAAGIVTQSADTVLTSTLATIPTV
                     TLTVGSAGTDGRQPLHVSASSSGATAVDWRIFFRTMGL"
     gene            1457..3181
                     /locus_tag="PKX01_16060"
     CDS             1457..3181
                     /locus_tag="PKX01_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77699.1"
                     /translation="MFAATAFAAAPYAALPADAVEDSGLPPEYLAGTAGAAIPGGAAA
                     VWTAVVTLHGADVSAQVLGEIVVEAEEGTARVASFTLRPPAGTAIYLPGWTGRAVTID
                     IAAADSSGPLYPMRLFTGVVDSPQYDPEARTVAVSATDDLQGALDALSRQAIDSLIGG
                     RWSPAVFDASAGGWAYAQDRLSTVRASLDLGPDGLPRLTPWPPAAPSLRFTGDHVLEG
                     SLAVNLADRQGLVNTVDITFGYRFPRLKAEGHALTYSYVDMTGFSQYVIDGKWFLQRS
                     QVEGAISSAGGTVVGAVTYTPMPSSPVAVGSGFWTPNPPVDATLCMGFSAVIAYDYAQ
                     TVEETHAVTVHCPAAVTATGIMRQAMRGALEGVYQDAEGWETKAKLYRADVESVQPQD
                     LPTVVSGKTNGLDPTLTADTNRAAATAAIETLIDVAKARIWASHRRNTVSAAVCLNPV
                     IDLDKTVEIDADGIHAIGKVQRLAHRMAPESGEATTTFSVAICAVAGVGTTHPETATA
                     AAAAPAAGQTNLPSSPVVTFNNGAAADHSFTLEFPAVSSGERAAATPTIASTFSAPLV
                     EDVFTVTL"
     gene            3178..3507
                     /locus_tag="PKX01_16065"
     CDS             3178..3507
                     /locus_tag="PKX01_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77700.1"
                     /translation="MSKDLTAALDALTREAAGLTSRADRALPAAKVAPAIPARAGTGK
                     PAASSGAGSIASPLTEASYAARLWHPATTIYSSDGLFSATRTPLKQVTMTDANGATVV
                     LNFAAPP"
     gene            3504..>5274
                     /locus_tag="PKX01_16070"
     CDS             3504..>5274
                     /locus_tag="PKX01_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77701.1"
                     /translation="MIDNWAHDAAATALVRFHPPATGLWRGANAYSPTDPGSLTCPDG
                     AVVSHPTPNGAWGGVSGDVTGNCRLLQVPSPPGDQRTAGQVAIDNSAHRQVRLYGLVA
                     GAAHTLYGKSIYNIDGSGGIGFIYVDPVRVRWHIQVTTVHAASECTATIKVRRFGHFD
                     PGRTFAVLTKVLSAPAGTLANNSGVALLAYRPDGGEAWFGAMRTPPAGGSNNPWGGGS
                     AQQDWPTCFWRLGVTGTGDETAVDHGLAFSWERDVPERVHEREVNQIVFGAAHVPGPA
                     EWTGDAPQQNGTWRYPYNKVNVGPQDGSGGIAPVPNCLFGWRDENYLTQERNVYTYRL
                     WPAYTAAGDLVWLTVRHEVYTSLHYVQTVSYAAYHVDVVNNVFQSPVVEGTDSYSWTL
                     DNDEWQRWTLSLGGMDILDETHTTVDVHTSGSGAETDRNTNRWSASYSQDYIGAVAVA
                     YYVVPFGVFDTPDYNTMDFRWDLPAGPIVLPGDGSRYDPMVALSVVRYSASLFGGFRR
                     TSVMHYPGGSALPYEDGVVCELVNAAGPEGAVSSFTEACINTGPAPTQYLFIAGQPNT
                     IGKFGSYHPVTHSIAWDTVPICWV"
BASE COUNT          834 a         1821 c         1762 g          857 t
ORIGIN      
        1 cgcgaggccc tcaagcgggg ggcgcggcga tgagcctgca gatcggaacg gtgagcatcg
       61 aggccctcga atcgctggac ctggatcaga cctacgaaat cctggggggc gaggcaatcc
      121 aacgcaccgt tggcggatct ggccttaagc agatgacctg ggcgcggctg cgggtgcaga
      181 cctcgggcca gggctggctg cccccggggc tggcggacct ggatacctcg cagcagatga
      241 ccctgcgctg catcgtcccc cgcgccatcc cctgcaccgg tctggtggcg accctgccgg
      301 cggcgcggcg cagcgatgcg ggctacctac cctatggcat cgccaccctg gccagcggcc
      361 aggccgtcaa gacaagcgcc gtcctggtgg gcaacgtggc caccctggcc gcggtggcgg
      421 gggccgtatc gtaccgggcg ctgtattacc cggagctgac tgtgtgggcc atgcgcccca
      481 gccacagcgg gagccgcagc gaggcgacgc accgctggga aatcacgtgt gaagaggtct
      541 aaccatgcca gaacttaatt tagccccccg cgtccgcgag acgagcacgt cctcgggcaa
      601 cggtgccctg accctggccg gcgcgccccc cgggtttttt ccgttttccg ccgtcgggtc
      661 tggggcagcc agggaagtgc cttacttgat cgagtggggc agcggcgcca gcgcgggagc
      721 agaatgggga atcggaaccc tcaatgcggc cgggacgctg ctgacccgga cctttgtgct
      781 gggccacacc ccatcaaccc tgcgggcggg cccgggcacc acgccggtgg acctgcccgg
      841 cgggacaaaa acggtgagtc tggccgccat cgacaccatg gcggtggcgg cgtgcccgga
      901 cctgacgcag accaccctcc catccccccg ggcagacggg gtgctggcta tggcggtggg
      961 gatcggctcc tcggcggtgg gggactggtc gtgcgcgttc ggcccttatg cgtctgcgcg
     1021 tcacccgtcc tcggtggtcg tcggcccggg cttgagcata gggccaaaga gcgccacgct
     1081 cgccgcggga gacgggctcg tggcggagca gtgcttcgct ggcaatgcgg cctccggcgc
     1141 gccgtttgtt ttcgacggcc ccacgctcgc gctgagcagc gatgcgccat actggatcga
     1201 gatgacggcc atcgcctgta acgccgcgat gacgcaattc cgggcaatcc gccgtaccgt
     1261 gttcgcggcg gcggcgggca tcgtgacgca gagcgcggac acggtgctga ccagcaccct
     1321 ggcgacgatc ccaaccgtca ccctgaccgt gggcagcgcc gggacggacg gtcgccagcc
     1381 cctgcacgtc tcggcgtcaa gctctggagc gacggccgtg gattggcgga ttttttttag
     1441 gacgatgggg ctgtaagtgt ttgccgccac cgccttcgcc gccgccccct acgccgcgct
     1501 gcccgcggac gcagtggagg attccggcct gccgccggag tacctggccg gcacggcagg
     1561 ggcggcgatc cccggcgggg ccgctgcggt gtggacggcg gtggtgaccc tgcacggcgc
     1621 ggatgtgtcc gcccaggtgc ttggggaaat cgtcgtcgag gcggaggagg gcaccgcccg
     1681 cgtagcctcg tttacgctgc gcccccccgc cggaacggcg atctatctgc ccgggtggac
     1741 gggccgggcg gtcaccatcg acatcgccgc cgcagactcc tccggcccgc tctaccccat
     1801 gcgcctgttc accggggtgg ttgacagccc gcagtacgac ccggaggcgc gcaccgtcgc
     1861 cgtgagcgcc acggacgatc tgcaaggggc gctggatgcc ctgagtcgcc aggccataga
     1921 cagcctgatc ggcgggcgct ggtcgccggc cgtgttcgac gcctccgccg gcggctgggc
     1981 ctatgcccag gaccgactct ccacggtgcg agcgtccctc gatctggggc cagacggcct
     2041 gccgcggctg acgccctggc cgcccgccgc gcctagcctc agatttacgg gggatcatgt
     2101 tctggaggga tcgctggcgg tgaacctcgc agaccgccag gggctcgtca atacggtgga
     2161 cattaccttc ggctaccgtt tcccgcggct caaggcggag gggcacgcct tgacctacag
     2221 ctatgtcgac atgacgggtt tttcgcaata cgtcatcgac ggcaaatggt tcctgcagcg
     2281 ctcccaggtg gagggcgcga tttcatccgc cggcgggacg gtggtcgggg ccgtgaccta
     2341 tacccccatg cccagcagcc ccgtggccgt cgggtccgga ttctggaccc cgaatccgcc
     2401 cgtcgatgcg accctgtgca tgggcttttc cgctgtcatt gcctacgact atgcgcagac
     2461 ggttgaggag acgcacgcgg tgacggtgca ttgccctgcc gcggtgacgg ccaccggcat
     2521 catgcggcaa gcgatgcgcg gcgccctgga gggcgtgtat caagacgccg aagggtggga
     2581 gaccaaggcc aagctctatc gcgccgacgt ggagtcggtg cagccccagg atctgcccac
     2641 ggtggtgtcc ggcaaaacca atggtctgga cccgaccctg acggcagaca ccaaccgggc
     2701 cgccgctacc gcggccatcg aaacgctgat cgacgtggcc aaggcccgca tctgggcctc
     2761 gcaccgccgc aataccgtga gtgcggccgt gtgcctcaat ccggtgatcg atctggataa
     2821 gacagtggag atcgacgccg acgggatcca cgccatcggc aaggtgcagc gtctggccca
     2881 ccgcatggct ccggagtctg gcgaggcgac gaccactttt tcggtggcca tctgcgccgt
     2941 ggccggggtc gggacgacgc acccggagac ggctaccgct gccgccgccg cgcccgcggc
     3001 ggggcagacc aatctcccat cgtcgcccgt ggtgacgttc aacaacggcg ctgccgccga
     3061 ccacagtttc accctcgaat tccctgcggt gtcctccggc gagcgggccg cggctacgcc
     3121 gaccatcgcc agcaccttct ccgccccgct ggtcgaggac gtttttacgg tgaccctatg
     3181 agcaaagacc tgaccgcggc cctggacgct ctgacccgcg aggccgccgg actcaccagc
     3241 cgggcggatc gggctctgcc tgccgccaag gtcgcgccgg ccatcccggc cagggccggc
     3301 acggggaagc cggcggcatc gtccggcgcc gggtccattg ccagccctct gactgaggcc
     3361 agctatgccg ccaggctgtg gcatccggct accacgatct actcgtcgga cgggctgttt
     3421 tctgcgactc gaacccctct caagcaggtc accatgacag acgccaatgg cgcgaccgtg
     3481 gtgcttaatt tcgcggcgcc gccatgatag acaactgggc gcacgatgcg gccgcgacgg
     3541 cactggtgcg gtttcacccc ccggctaccg ggctgtggcg gggcgctaat gcctacagcc
     3601 cgaccgatcc ggggagcctg acctgcccgg atggcgccgt cgtctcgcat ccgacgccga
     3661 acggtgcatg gggaggtgtc tccggagacg tgaccggcaa ttgccgcctg ttgcaggtgc
     3721 cgtcgccccc cggcgaccag cgcaccgccg ggcaggtggc catcgataac agcgcacatc
     3781 gccaggtgcg cctctacggc ctggtggctg gcgcggcgca caccctgtac ggcaagtcga
     3841 tctacaatat cgatggcagc ggtgggatcg gcttcatcta cgtcgacccc gtccgggtcc
     3901 gctggcacat ccaggtgacc acggtccacg ccgccagcga atgcacggcg acaatcaagg
     3961 tgcgccgctt cgggcacttt gatccgggcc gcacctttgc ggtgctgacc aaggttctga
     4021 gcgccccggc gggcaccctg gccaataatt ccggggtggc cctgctcgcc tacagacccg
     4081 acggcggcga agcctggttc ggcgccatgc gtaccccccc ggccgggggc agcaataacc
     4141 cctggggggg cggctccgcg caacaagact ggccgacctg cttttggcgc ctcggcgtga
     4201 ccggcacggg cgacgaaacc gccgtcgatc acggactcgc cttctcctgg gagcgcgacg
     4261 tgccggagcg ggtccatgaa cgtgaggtga accagatcgt ttttggcgcg gcgcatgtcc
     4321 cggggcctgc ggagtggacc ggcgacgcgc cacagcaaaa cggcacctgg cgatacccgt
     4381 acaacaaggt caacgtgggg ccgcaggacg gcagcggagg aatcgccccc gtgcccaatt
     4441 gcctatttgg gtggcgcgac gaaaactacc tgacccagga gcgcaacgta tacacctatc
     4501 gcctgtggcc ggcgtatacc gccgcgggcg atctcgtttg gctcaccgtg cgccatgagg
     4561 tctacacatc tttgcactat gtgcaaaccg tctcctacgc cgcctatcat gtcgatgtcg
     4621 tcaataacgt atttcagagc cccgtcgtag agggtacgga ttcctactcc tggactctcg
     4681 ataacgacga gtggcagcgg tggacgctct cgctgggcgg gatggacatt ttggacgaga
     4741 cgcacactac agtggacgtc cacacctccg gatctggcgc ggagaccgac cgcaacacca
     4801 accggtggag cgccagctac tcgcaggact acatcggggc cgtcgctgtg gcctactacg
     4861 tggtgccgtt cggcgttttc gacacgcccg actacaacac catggatttt cgctgggatc
     4921 tccccgccgg cccaatcgtc ctgcccggcg acggatcgcg ttacgatccc atggtcgccc
     4981 tatccgtggt gcgctattcc gccagccttt tcggcgggtt ccgccgcaca tcggtcatgc
     5041 actacccggg tggcagcgca ctcccgtacg aggacggcgt tgtctgcgag ctggtcaacg
     5101 ccgccggacc cgagggcgct gtctcctcgt tcaccgaggc ctgcatcaat accggccccg
     5161 caccgaccca gtatctgttc atcgcggggc agcccaatac aatcgggaag tttggctctt
     5221 accaccccgt cacccactcg attgcgtggg ataccgtgcc gatctgctgg gtat
//
LOCUS       DAOQPA010000175         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_9632_length_5249_cov_25.795341, whole genome shotgun sequence.
ACCESSION   DAOQPA010000175 DAOQPA010000000
VERSION     DAOQPA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_5249_cov_25.795341"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKX01_16075"
     CDS             complement(<1..194)
                     /locus_tag="PKX01_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HMZ77702.1"
                     /translation="MIRSFDLLRRMRAAVTSVLLLSATGSYALNTATIVGSVASPDCL
                     EYRVVGICYWLYCTWTGCTVR"
     gene            complement(191..637)
                     /locus_tag="PKX01_16080"
     CDS             complement(191..637)
                     /locus_tag="PKX01_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HMZ77703.1"
                     /translation="MPASSSRFASGWRTLGLAVALPASLAVFSPASFAADVVIITDSR
                     HPVRTMGGEQLIELDEAPRIEAELSAALPADPGQATAIVKRRLSQGGADLQRRIATEY
                     QGVADAWSLGITTIPAVVVDQRYVVYGEPDVARAIARIEQHRRAEP"
     gene            complement(801..1295)
                     /locus_tag="PKX01_16085"
     CDS             complement(801..1295)
                     /locus_tag="PKX01_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HMZ77704.1"
                     /translation="MSFAVNDSCLESLSAIAAQHEDWIIQQAIVLLERRVFKAGPCLS
                     QPAAVRDYLRLKLVAEPNEIFAAVFLDSLHQVLAYEPLFRGTINATSVYPRVVVQRVL
                     ELNAAAVVFAHQHPSGVSEPSSADRMLTQQLQAALALIDVRVLDHIIVGQGAPFSFAE
                     SGLL"
     gene            complement(1472..2236)
                     /locus_tag="PKX01_16090"
     CDS             complement(1472..2236)
                     /locus_tag="PKX01_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ77705.1"
                     /translation="MEQKRSSIPMQVQAFRRRRWRARWPWALGAVLVALLLIWLVSRS
                     PGESTPQTSAPVSETQVAGPPWQMGNPAGRFTLTLYADLECPFCRSYFPVLKRWVAGN
                     ADVALQWHHLPLAAHEPAASAEARLVECAGEAGGHPAFWQAVEWVYVHTHSDGQGLPE
                     DLRHPALTPAIEQCIASERPDAAIRTQTAEATNSGVTATPSLRLHDRETGKAILLQGP
                     IEGDALLSAMDMLAAGDPAATPTSEMPADVVGDMPR"
     gene            complement(2261..5143)
                     /locus_tag="PKX01_16095"
     CDS             complement(2261..5143)
                     /locus_tag="PKX01_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HMZ77706.1"
                     /translation="MRWKLPWPKPAALKLAASGAGDDEQPDGWQRHIEALRQAGIPEP
                     GSAVRGRKPATEADEQALYEVAPSFVELLPWVEFLPESRSMLLEDGQSVAAFFELVPL
                     GTEGREPGWLAHARDALENALQDSFDELDENPWVLQLYAQDEPSFDQYMQTLRDYVQP
                     RARGTAFTEFYLRFFGHHLRAVAKPGGLFEDTVVTRLRWRGQTRRVRMVIYRRATGQA
                     SRRGQTPEQMLNIVCDRLCGGLANAGIQARRMVAADVHDWLLRWLNPRPAMLGPSTED
                     RERFYALARYPDETEAGEIELASGRDFSQRLFFGQPRSDVEHGTWYFDGMPHRVLITD
                     RLRMPPGTGHLTGETRKGDAINTLFDQMPEDTLLCLTMVATPQDVLESDLNHLAKKAV
                     GETLASEQTLKDVHEARSLIGSAHKLYRGTLAFYLRGRDEAELDRRGLDLANVMLNAG
                     LQPVREDDEVAPLNSYLRWLPCCYNPGKDRRRWYTQLMFAQHAANLSPVWGRAQGTGH
                     PGITMFNRGGGPITFDPLNRLDRQMNAHLFLFGPTGSGKSATLNNLLNQVTAIYRPRL
                     FIVEAGNSFGLFSDFARRLGLTVNRVKLAPGSGISLAPFADARRLIETPSDVQTLDAD
                     ALDEDLPPDASAMEADEQRDVLGELEITARLMITGGEDKEEARMTRADRSLIRQCILD
                     AAEHCVAEKRTVLTRDVRNALRTRGQDPTLPEMRRVRLLEMADAMDMFCQGTDGEMFD
                     RDGTPWPEADITLVDLATYAREGYNAQLSIAYISLISTVNNIAERDQYLGRPIINVTD
                     EGHIITKNPLLAPYVVKITKMWRKLGAWFWLATQNIDDLPRAAEPMLNMIEWWICLSM
                     PPDEVEKIARFRELSPAQKALMLSARKEAGKFTEGVILSKSMEVLFRAVPPSLYLALA
                     QTEPEEKAERYQLMQQHGVSELDAAFKVAEKIDRARGIESPALALP"
     gene            complement(5143..>5249)
                     /locus_tag="PKX01_16100"
     CDS             complement(5143..>5249)
                     /locus_tag="PKX01_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HMZ77707.1"
                     /translation="LAGTDPVPVPGYTTVFPLYQRIQYAMPGERVEDY"
BASE COUNT          872 a         1718 c         1773 g          886 t
ORIGIN      
        1 cgcaccgtgc agccggtcca ggtgcagtag agccagtagc agatacccac cacccggtac
       61 tccaggcaat cgggcgaagc cactgaaccc acgatggtcg ccgtgttcag ggcgtagctg
      121 cccgtggcgc tgagcagcag cacggaggtg acggcagccc gcatgcggcg cagaaggtcg
      181 aatgagcgga tcacggttcg gccctccggt gctgttcgat gcgcgcgatg gcgcgggcca
      241 cgtcaggctc gccatagacc acgtagcgct gatccaccac gacagccgga atggtggtga
      301 tgcccaggct ccatgcgtcg gcaacgccct ggtattcggt ggcgatgcga cgctggaggt
      361 cagcgccccc ctggctcagc cggcgcttca cgatggctgt tgcctgtccg ggatcggcgg
      421 gcagcgccgc agaaagctcc gcttcgatcc ggggcgcttc atccagctcg atcagctgct
      481 cgccacccat ggtcctgacc gggtgacggc tgtcggtgat gatcaccaca tcggcggcga
      541 agctggccgg gctgaacacg gccagggaag ccggcagcgc aacggccagg ccaagggttc
      601 gccagcccga tgcgaacctg gatgaagatg ctggcatgtc gagcgccccc aagagttgtc
      661 aagggccata gtcaaacgcc gaacgccatg cggccccaac aaacaatgcg catcgcggac
      721 tgcccgcata cctgcttgtc ttgcgccaat gaaaagcgga ggccgaagcc tccgcgtgga
      781 tcaaggagcc ggtgccattg ctacagcagg ccggattccg caaaggagaa cggggcaccc
      841 tggccgacga tgatgtggtc cagtacccgc acatcgatga gcgccagcgc ggcttgcagt
      901 tgctgggtca gcatgcgatc cgcgctggac ggctcggaga cgcccgaagg atgctggtgg
      961 gcgaagacca ccgcggcggc attcaactcc agcacacgct gcacgacgac acgcggatag
     1021 accgaagtcg cgttgatcgt gcccctgaac agcggctcgt aggccagcac ctggtgcagg
     1081 ctgtccagga acacagcggc gaatatctca ttgggctcag cgaccagttt cagacgcagg
     1141 tagtcccgta cagcggccgg ttggctgagg cacggtccag ctttgaagac ccgtctctcc
     1201 agcagcacga tggcttgctg gatgatccag tcctcgtgtt gggcagcgat ggcggaaagc
     1261 gactccaggc aggagtcatt gacggcgaaa gacatggcga acctccagac ggtgagatcg
     1321 gagggcactc gccctgggag ggcaagccct cctggggaac gaacaaggtg catccatcac
     1381 cgcggcagcg gtgatcgttc gcggccagga tgcgaggcga acggggttgt ggtcagcgca
     1441 gcggactgcg ccgtagcctt gaagaccggg gctacctggg catgtcgccg acgacgtcgg
     1501 caggcatttc cgatgtgggt gtggcggcgg gatcgccggc cgcgagcatg tccatggccg
     1561 acagcaaggc atcgccctcg atcgggccct gcagcaggat cgccttgccg gtttcgcgat
     1621 cgtgcagccg cagcgacggc gtggcggtca cgccgctgtt cgtggcttcc gcagtttggg
     1681 tacgaatcgc cgcgtcgggc cgctcgctcg caatgcactg ttcgatggct ggcgtcaggg
     1741 cggggtggcg caggtcttcg ggcaagccct ggccgtcgct gtgtgtgtgg acatagaccc
     1801 actcgacagc ctgccagaag gcgggatggc cgccggcttc gcccgcgcat tccaccaagc
     1861 gcgcttcggc ggacgcggcc ggctcatgcg cggccaacgg caggtggtgc cattgcaagg
     1921 ccacgtccgc attgccggct acccaacgct tgaggacagg gaaataggac cggcagaacg
     1981 ggcattcgag gtccgcgtag agcgtcagcg tgaaacgacc tgccgggttg cccatctgcc
     2041 aaggaggccc ggccacctgc gtctcgctga caggcgccga ggtttggggc gtggactcgc
     2101 ctggcgaacg ggacacgagc cagatcagca gcagcgcaac cagcactgcg cctaacgccc
     2161 atggccagcg ggcccgccag cgccgacggc ggaacgcctg gacctgcatc ggaatggatg
     2221 agcgtttctg ttccatggcg ttctccggtg cacggcagat tcagggcagg gccagggccg
     2281 gcgactcgat gccgcgtgcc cggtcgatct tctcggccac cttgaaggcg gcatccaact
     2341 cgctgacgcc gtgctgctgc atgagctggt agcgctcggc cttctcttcg ggttcggtct
     2401 gcgcgagcgc gagatacagg ctcggcggca cggcgcggaa gagcacctcc atgctcttgg
     2461 agaggatgac gccctcggtg aacttgcccg cttccttgcg tgccgaaagc atcagcgcct
     2521 tctgcgccgg cgagagttcg cggaatcgtg cgatcttctc cacctcgtcc ggcggcatcg
     2581 acaggcagat ccaccactcg atcatgttga gcatgggctc tgcggcgcgc ggcagatcgt
     2641 cgatgttttg cgtcgccagc cagaaccagg cgcccaactt gcgccacatt tttgtaattt
     2701 tcacgacata cggcgcgagc agcgggttct tcgtgatgat gtggccttcg tcggtcacgt
     2761 tgatgatcgg gcggccgaga tactggtcac gctccgcgat gttgttcacc gtgctgatga
     2821 ggctgatgta cgcgatcgag agctgcgcgt tgtagccctc acgggcatag gtcgcgagat
     2881 ccaccagcgt gatgtcggcc tcgggccacg gcgtgccgtc gcggtcgaac atctcgccgt
     2941 ccgtgccttg gcagaacatg tccatcgcgt ccgccatctc cagcagccgc acgcgccgca
     3001 tctcgggcaa cgtcgggtcc tggccgcggg tgcgcagcgc attgcgcacg tcgcgcgtga
     3061 gcaccgtgcg cttctcggcc acgcagtgct cggcggcgtc gaggatgcac tggcggatca
     3121 gcgagcggtc ggcgcgcgtc atcctggctt cctccttgtc ctcgccgcca gtgatcatca
     3181 gccgcgccgt gatctccagt tcgcccagca cgtcgcgctg ttcgtccgcc tccatggctg
     3241 aggcatcggg tggcaggtct tcgtccagcg catcggcatc gagcgtttgc acatcgctgg
     3301 gcgtttcgat cagccggcgc gcgtcggcga acggcgctag gctgatgccc gatccggggg
     3361 ccagcttgac ccgattcacg gtcaggccca ggcgcctggc gaagtcgctg aacaagccga
     3421 agctattgcc ggcttccacg atgaagaggc gcggccgata aatggccgtg acctggttca
     3481 gcagattgtt gagcgtggcc gatttgccgg aacccgtcgg gccgaacagg aacaggtggg
     3541 cgttcatctg ccggtccagg cgattgagcg gatcgaaggt gatcggcccg ccgccgcggt
     3601 tgaacatcgt gatgcctgga tgccccgtac cctgggcacg gccccacacc ggcgacaggt
     3661 ttgccgcgtg ctgggcgaac atcagttgcg tgtaccaccg gcgccgatcc ttgccggggt
     3721 tgtagcagca cggcagccag cgcaggtagc tgttgagcgg cgccacctcg tcgtcctcgc
     3781 gcaccggctg caagccggcg ttgagcatca cgttcgccag gtccaggccg cgccgatcca
     3841 gttccgcctc gtcgcgcccg cgcaggtaga acgccagcgt gccccggtag agcttgtgcg
     3901 cgctgccgat cagcgagcgg gcttcatgca cgtccttgag cgtctgctcc gacgccagcg
     3961 tctcgcccac ggccttcttc gccaggtggt tcagatccga ttccaggaca tcctggggcg
     4021 tggcaaccat cgtgagacaa agcaaggtgt cttccggcat ctggtcgaac agcgtgttga
     4081 tcgcatcgcc cttgcgggtc tcgccggtta gatgtcccgt gccgggcggc atgcgcagcc
     4141 ggtcggtgat cagcacacgg tgcggcatgc cgtcgaagta ccaggtgccg tgctccacgt
     4201 cggagcgtgg ctggccgaag aacagccgct ggctgaaatc ccgcccgctc gccagttcga
     4261 tctcgccggc ctcagtctcg tccgggtagc gcgccagtgc gtagaagcgc tcccggtcct
     4321 ctgtactggg cccgagcatc gcggggcgcg gattgagcca ccgcagcagc cagtcgtgga
     4381 cgtcggctgc gaccatgcgc cgtgcctgga tgccggcgtt cgccagcccg ccacacaggc
     4441 ggtcgcagac gatgttcagc atctgctcgg gcgtctggcc gcggcggctt gcctgcccgg
     4501 tggcacggcg atagatcacc atgcgcaccc gccgcgtctg gccgcgccag cgcagccgcg
     4561 tgaccaccgt gtcctcgaac aggccgccgg gcttggccac cgcacgcaga tggtggccga
     4621 agaagcgcag gtagaactcg gtgaacgctg tcccgcgggc acgtggctgc acgtagtcac
     4681 gcagggtctg catgtactgg tcgaaactcg gctcgtcctg ggcatagagc tggagcaccc
     4741 aggggttctc gtccagctca tcgaagctgt cctgcagcgc gttctccaag gcgtcgcggg
     4801 cgtgggcaag ccagcccggt tcccggccct cggtccccag cggcaccagc tcgaagaagg
     4861 ccgccaccga ttggccgtcc tccagcaaca tggaccttga ctcgggcaag aactcgaccc
     4921 agggcagcag ttccacgaag gacggtgcga cctcgtacag cgcctgctcg tcggcctccg
     4981 tcgccggttt gcggccccgg actgccgagc cgggttcggg gatgccggct tggcgcaagg
     5041 cctcaatgtg gcgctgccag ccgtccggct gctcgtcatc accagcgccg gatgcggcca
     5101 gcttcagcgc ggccggctta ggccagggaa gtttccagcg catcagtagt cctccacgcg
     5161 ctcacccggc atcgcgtact gaatgcgctg gtacagcggg aagacggtcg tgtagccggg
     5221 caccggcacg ggatcggtgc cggccaggt
//
LOCUS       DAOQPA010000176         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_9958_length_5141_cov_16.704090, whole genome shotgun sequence.
ACCESSION   DAOQPA010000176 DAOQPA010000000
VERSION     DAOQPA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9958_length_5141_cov_16.704090"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1295
                     /gene="can"
                     /locus_tag="PKX01_16105"
     CDS             153..1295
                     /gene="can"
                     /locus_tag="PKX01_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HMZ77708.1"
                     /translation="MAVTLRVIGAEDRAEIEHVRQFFRTYAGWLGVDLDFQNFAAEME
                     TLPGAYAAPTGRLFFAELDGRPAGCVGIRAATEGVCEMKRLYVEPECRGTGVGRELAL
                     AGIKAAKAMGYRKIMLDTLPAMRIAVKLYRELGFKEAPAYYPTPVEGTMFLALDLENW
                     SEAEIYNENLFHLFDYNLAWARKMRQVDPGFFEKLSTLQTPEYLWIGCSDSRVPANEI
                     IGLLPGEVFVHRNVANVVVHTDLNCLSVIQFAVDVLKVKHIMVVGHYGCGGVRAALQR
                     DRVGLVDLWLRHVQDVHVKHYGRVDMLPPELRHDRLCELNALEQVVNVCQTVVVQDAW
                     KRGQRLTVHGWVYGLKDGLIRDLGINVSRRDDLVARYQAALEALEA"
     gene            1404..1844
                     /locus_tag="PKX01_16110"
     CDS             1404..1844
                     /locus_tag="PKX01_16110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ77709.1"
                     /translation="MAKAIRLRDDIDPATLTARGTGHLPGWYGLSVTELEPGRLRARM
                     TVRPEMLAPNGFLHAASVVALADTSAGYATVAHLPDGATSFTTLELKSNFFATVTEGE
                     LVCDATAVHLGRSTQVWDAEVRAADGARLALFRCTQMILWPKAA"
     gene            1859..2473
                     /locus_tag="PKX01_16115"
     CDS             1859..2473
                     /locus_tag="PKX01_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HMZ77710.1"
                     /translation="MTASLKAPLDFYFDFSSPYGYLAAEKIDALAAAHGRSVLWHPIL
                     LGVTFKATGLAPLPAIPLKGEYSLRDIARSARFLGVPYRKPTVFPIPTQHAARAFLWL
                     NDRDPQQARAFGLAAYRAYFTEDVNISDLGAVLEIAAAQGVDRDALSAVLAGAEIKAR
                     LVAEVELAMARGVFGSPFIIVDGESFWGADRLPQVARWLAEGGF"
     gene            2569..3171
                     /locus_tag="PKX01_16120"
     CDS             2569..3171
                     /locus_tag="PKX01_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HMZ77711.1"
                     /translation="MSAPIDFYFDFASPYGYLASEQIDALAARHGRAVMWHAIVLDAQ
                     FQPQGGMKIPAALMRTEYVRRDIERSAAYYGIPYKAPSPQAAHTEHAARAFQWLNDRQ
                     PEQARAFAHAVFRAYFVDSRNIAEPAVLLEIAEALKLDREDIAGALSDAATKARLKAE
                     IDLAEARGVFGSPFFIVEGEGFWGNDRLPQLQRWLEKGPY"
     assembly_gap    3294..3393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3394..3921
                     /locus_tag="PKX01_16125"
     CDS             <3394..3921
                     /locus_tag="PKX01_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ77712.1"
                     /translation="AAAEFLGTTLAERGIELVYGGGNVGLMGVVADACLAAGGRVVGV
                     IPRALLDWEVGHEGLTRLEVVDSMHTRKARMAELADGFIALPGGLGTFEELFEILTWA
                     QLGFHAKPVGLLDTAGYYLPLLQMIERGVAEGFMKAENRGLLLAEDNLFALLRAMGQY
                     HPPTVSKRIREEKHL"
     gene            3976..>5141
                     /locus_tag="PKX01_16130"
     CDS             3976..>5141
                     /locus_tag="PKX01_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ77713.1"
                     /translation="MSVIRSSINPRSAEFQANAAALRTLVADLRDTVGRVALGGGAAA
                     RDKHTARGKLLPRDRVDHLLDPGAPFLEIGQLAAWGLYDNEAPCAGLIAGIGRVSGVE
                     CMILANDATVKGGTYYPLTVKKHLRAQEIAEQNGLPCVYLVDSGGAFLPRQDEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMSDETVIVRNQGTIFLGGPPL
                     VKAATGEEVSAEDLGGGDVHTRISGVADHLAENDAHALYIARRIVANLNRVKPVALRL
                     ADAEEPLYDPEEIYGILPSDTRKPYDVREIIARVVDGSRFDEFKARYGTTLVCGFAHL
                     YGYPVGIVANNGILFGESAQKGAHFIELCAQRGIPLVFLQNITGFMVGRKYENGG"
BASE COUNT          787 a         1825 c         1624 g          805 t
ORIGIN      
        1 gccggcacca gcgagatccg ccgcatgctg atcgggcgcg agctgttcgc cgagaccgcc
       61 tgagggatgc ttcggtgcac aacggggatg aatcggagcg ccgggacggg tatcgtgtcg
      121 gattcttgcg tattcatcgg tgagtggatc ccatggctgt gactcttcgc gtaatcggtg
      181 ccgaggaccg ggccgaaatc gagcatgtgc gccagttttt ccgcacttac gcgggctggc
      241 tgggcgtgga tctggatttc cagaactttg cggccgagat ggagaccctg cccggtgcct
      301 atgccgcacc gaccgggcgc ctgttcttcg ccgagctgga cggcaggcct gccggctgcg
      361 tgggcattcg tgcggcgacc gaaggcgtct gcgagatgaa gcgcctctac gtggaacccg
      421 agtgccgcgg caccggtgtc ggtcgcgaac tggccctggc cggcatcaag gccgccaagg
      481 cgatgggcta tcgcaagatc atgctcgaca ccctgccggc catgcgcatc gcggtgaagc
      541 tgtaccgcga gctgggcttc aaggaggcac ccgcctacta tccgacgccg gtggaaggca
      601 ccatgttcct cgccctcgat ctggaaaact ggagcgaggc cgagatctac aacgagaacc
      661 tgttccacct cttcgactac aacctggcct gggcgcgcaa gatgcgccag gtggatcccg
      721 gcttcttcga gaagctgtcc accctgcaga cacccgaata cctgtggatc ggttgttccg
      781 attcccgcgt gccggccaac gagatcatcg gcctgctgcc gggcgaagtc ttcgtccacc
      841 gcaacgtggc caacgtggtg gtgcacaccg acctcaactg cctgtcggtg atccagttcg
      901 ccgtggatgt gctcaaggtc aagcacatca tggtggtcgg acactacggc tgtggcggcg
      961 tgcgggcggc cctgcagcgc gaccgggtgg ggctggtgga tctgtggctg cgccacgtgc
     1021 aggacgtgca cgtcaaacac tacggccgcg tcgacatgct gccgcccgag ctgcgccacg
     1081 accgcctgtg cgagctcaac gcgctggagc aagtggtcaa cgtgtgccag accgtcgtcg
     1141 tgcaggacgc ctggaagcgc ggccagcgcc tcacggtgca tggctgggtg tatggactga
     1201 aggacgggct gatccgcgat ctggggatca acgtcagccg tcgtgacgat ctggtggccc
     1261 gttatcaggc cgccctggag gccctcgaag cctgaacgcc gcggcctgct gcgcggcgag
     1321 gacgattcga ccccggacac acgggggacc ctcgcggttg gaccggatgg ccggtccgcc
     1381 gccccgacaa ggagacgacg cagatggcca aggcaatccg cctgcgggac gacatcgatc
     1441 ccgcaaccct caccgcccgg ggcaccgggc acctgcccgg ctggtacggg ctcagtgtca
     1501 ccgagcttga gccgggccgt ctccgcgccc gcatgacggt gcgccccgag atgctggcgc
     1561 ccaacggctt cctgcacgcc gcctcggttg ttgccctggc cgacaccagt gccggctatg
     1621 ccaccgtcgc ccacctgccc gacggggcga cgagcttcac cacccttgag ctgaagagca
     1681 acttcttcgc cactgtcacc gagggcgagc tggtctgcga cgcgaccgcc gtgcatctgg
     1741 ggcgcagcac ccaggtgtgg gacgcggaag tccgcgccgc cgatggcgcc cgcctggccc
     1801 tgttccgctg cacccagatg atcctgtggc ccaaggccgc gtgacgccgg agaccgccat
     1861 gaccgcttcc ctgaaggccc ccctcgactt ctacttcgat ttctcctcgc cctacggcta
     1921 ccttgccgcc gagaagatcg acgccctggc cgccgcccac ggccgcagcg tgctgtggca
     1981 cccgatcctg ctgggcgtca ccttcaaggc caccggcctc gcgccgctgc cggcgatccc
     2041 cctcaagggc gaatactccc tgcgcgacat cgcccgctcg gcgcgatttc tgggcgtgcc
     2101 ttaccgcaag cccacggttt tcccgattcc cacccagcac gccgcccgtg ccttcctgtg
     2161 gctcaacgac cgcgacccgc agcaggcccg cgccttcggc ctggcggcct accgggccta
     2221 cttcaccgag gacgtgaaca tctccgacct gggcgccgta ctggagatcg ccgcagccca
     2281 gggcgtggac cgggacgcac tgtctgccgt gctcgccggt gccgagatca aggcacggct
     2341 ggtggccgag gtggagctgg cgatggcccg cggcgtcttc ggctcgccct tcatcatcgt
     2401 ggacggcgag tccttctggg gcgccgatcg cctgccccag gtggcgcgct ggctcgcgga
     2461 gggcggcttc tgacaggctc tgacggggct gtcacgtggc gccgccgggc gccgtggata
     2521 atgacgttcg acgccccctc aacccgcctc accggagacc gactgcccat gtccgccccc
     2581 atcgacttct atttcgactt cgcctcgccc tacggttatc tcgcgtccga gcagatcgac
     2641 gccctggcgg cccgccatgg ccgcgcggtg atgtggcacg ccatcgtgct cgacgcccag
     2701 ttccagcccc agggcggcat gaagatcccc gccgcgctga tgcgtaccga gtacgtgcgg
     2761 cgcgacatcg agcgctccgc cgcgtattac ggcatccctt acaaggcgcc ttcgccccag
     2821 gccgcccaca ccgagcacgc cgcccgcgcc ttccagtggc tcaacgaccg ccagccggag
     2881 caggcccgcg ccttcgccca cgcggtgttc cgcgcctact tcgtggacag ccgcaacatc
     2941 gccgagcccg ccgtgctgct ggagattgcc gaggcgctca agctggatcg cgaggacatc
     3001 gccggcgccc tctccgacgc cgccaccaag gcccgcctga aggccgagat cgacctggcg
     3061 gaggcccgtg gcgtcttcgg ctcgcccttc ttcatcgtcg agggtgaagg cttctggggc
     3121 aacgaccgcc tgccccagct gcagcgctgg ctcgaaaagg gtccctactg aggcgcccgc
     3181 gatgaaacgg atctgtgtct tctgcggctc ccggcccggc cagcggcctg aataccgcgc
     3241 cgccgccgaa ttcctcggca ccacccttgc cgagcgcggc atcgaacttg tttnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgccg ccgaattcct cggcaccacc
     3421 cttgccgagc gcggcatcga acttgtttat ggcggcggca acgtgggcct gatgggggtg
     3481 gtggccgatg cctgcctcgc tgccggcggg cgcgtggtag gcgtgatccc ccgcgccctg
     3541 cttgactggg aggtgggcca cgaagggctc acccgcctgg aggtggtgga ctccatgcac
     3601 acccgcaagg cgcgcatggc cgaactggcc gacggcttca tcgccctgcc cggcgggctc
     3661 ggcaccttcg aggagctctt cgagattctc acctgggccc agctcggctt ccacgccaag
     3721 ccggtcggac tgctcgacac cgcgggctac tacctgccgc ttctgcagat gatcgagcgg
     3781 ggcgtggctg agggattcat gaaggccgag aaccgcggcc tgctgctcgc cgaagacaac
     3841 ctgttcgccc tgttgcgggc catgggccaa taccacccgc ccaccgtgtc caaacggatc
     3901 cgggaggaga agcacctgta gcgaccatcg gccgaccccg cggcccccat aaaaacacat
     3961 cagggaagag gagaaatgag cgtcatccga tccagcatca acccccgttc ggccgaattc
     4021 caggccaacg ccgccgccct gcgcaccctg gtggcggacc tgcgcgacac tgtcggccgc
     4081 gtcgccctgg gcggcggtgc agcggcccgc gacaagcaca ccgcccgcgg caagctgctg
     4141 ccccgcgacc gggtcgacca cctcctcgat cccggcgccc cgttcctgga gatcggccag
     4201 ctcgccgcct ggggcctgta cgacaacgag gcgccctgcg ccggcctcat cgccggcatc
     4261 ggccgggtga gtggcgtgga atgcatgatc ctcgccaacg acgccaccgt gaagggcggc
     4321 acctactacc cgctgaccgt caagaagcac ctgcgcgccc aggagatcgc cgagcagaac
     4381 ggcctgccct gcgtctatct ggtggattcc ggcggtgcct tcctgccccg ccaggacgag
     4441 gtcttcccgg atcgcgacca cttcgggcgg atcttctaca accaggccaa cctgtccgcc
     4501 cgcggcatcc cgcagatcgc cgtggtgatg ggctcgtgca ccgccggcgg cgcctacgtg
     4561 ccggccatga gcgacgagac cgtcatcgtg cgcaaccagg gcaccatctt cctggggggg
     4621 ccgcccctgg tgaaggccgc caccggcgag gaggtgagcg ccgaggacct gggcggcggc
     4681 gacgtgcaca cgcgcatctc cggggtggcc gaccacctgg ccgagaacga cgcccacgcg
     4741 ctgtacatcg cccgccgtat cgtggccaac ctgaatcggg tcaagccggt ggcgctgcgc
     4801 ctcgccgacg ccgaggagcc cctctacgac cccgaggaga tctacggcat cctgcccagc
     4861 gacacgcgca agccctacga cgtgcgcgag atcattgccc gcgtggtgga cggctcccgc
     4921 tttgacgaat tcaaggctcg ctacggtacc accctggtgt gcggcttcgc tcacctctac
     4981 ggctacccgg tgggcatcgt cgccaacaac ggcatcctct tcggcgaatc cgcccagaag
     5041 ggcgcccact tcatcgagct gtgcgcccag cgcggcattc ccctggtctt cctgcagaac
     5101 atcaccggct tcatggtcgg ccgcaagtac gagaacggcg g
//
LOCUS       DAOQPA010000177         5136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_9973_length_5136_cov_28.697304, whole genome shotgun sequence.
ACCESSION   DAOQPA010000177 DAOQPA010000000
VERSION     DAOQPA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9973_length_5136_cov_28.697304"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKX01_16135"
     CDS             <1..809
                     /locus_tag="PKX01_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HMZ77714.1"
                     /translation="VVGICYWLYCTWTGCTVRTSVKVRHYIPDAVVSSYSNTGENPWV
                     EVRAMSTPNPSAQAGGDGTTNEDHENNLAKFKNADVIGHPGVEVFNQFVSSSGYFCEG
                     AGTAFMPYLLSTLDTPAWRYNVPEMVYPEALIPGMREVGARSTLNLWGNVYPRGGFLH
                     QTDDHKAGAVVAQRAGDVVTRRGQIHVYQPLLANSRDGYWPAGALMEGDASTGKWQEL
                     TPVLSSSCTVFPRNGFLTQAQQGDYAWALWRPYACCERRGQVFLGSVDFY"
     gene            820..2214
                     /locus_tag="PKX01_16140"
     CDS             820..2214
                     /locus_tag="PKX01_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HMZ77715.1"
                     /translation="MKRPELMNPSAKVRRPLRPTALAGALALVCGLAWAQAGFQTSGP
                     VIGDEVMYSIGGGSAVSMGRAAGMRSIGVGLGWNSNLICGDMSIQTTLRNQLNGITNG
                     FQQIMSNVIQSATSAVASLPALIIQRADPGLYNLLTNGVLQARLDFDRSKLTCRAMAE
                     KMAETAGGQLGWSQMAEGMALRDAVSSTDAVSAIEQAETRRGNDGVPWVGGSNAGGAG
                     QSAIRVVGDVTRAGYNLVNGRSVTDTSSIAPASCASLSCQTWTSPQQATEWATRVLGE
                     QVQRTCDSCTKTETVPGVGLTPLIQEEYETKLEALQELVSGTRNTTFENLHAAGSTSL
                     PITRGVIEALRDEPDQDLLARRLASEVALSSVLEKALLLQRTLLTGKKEPNVAANQLA
                     VEAVNHESDTLDQEIRNLKTELELRRELANNSPMAIIQRHGTRAAGSRGIYEGDPVPD
                     RLDRLQRGNPGGTP"
     gene            2211..2558
                     /locus_tag="PKX01_16145"
     CDS             2211..2558
                     /locus_tag="PKX01_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77716.1"
                     /translation="MNAAWLRPRWLFSRRAAKALLLAVVIIAAAVGANLVGIYLVGSV
                     AGWERWLAASAGYFLVWRLCLYGATVYGWVWMRRRLLAREEDAQARRRLVRSEIAGVV
                     AIVALEASLLMQS"
     gene            2574..4097
                     /locus_tag="PKX01_16150"
     CDS             2574..4097
                     /locus_tag="PKX01_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HMZ77717.1"
                     /translation="MTLFTTDYLEYYLTLVSWIVNNGIWAVLVSSGVFALPFVAIIVQ
                     EWLKARAEGADEGNKGVLSAARIENRVFVAIVVVMFAGIPFIDVDLNTIQYDSSRSAQ
                     CQVSVPQPTDTGWSQSFSTINNQSAKVPVWWAFMHALSRAVTSASVAAIPCGTDLRQM
                     RMEIDATRIDDPVLAQEVADFSRDCYGPARAKLFMQRPQLDEQQMHDVTWIGSRFFTD
                     TGGYYDTYRSSTPRDDWPYDSTRDAGLAQVGSGGGYPTCRQWWADGGNGLRARLLGQV
                     DPNLLNRLAGWAGFLSRAEVDDSVIRAIASPRQQKLNQGSVYTDYGGQIDKTLPNIVT
                     RATGDVGMAVGAIAAFPAMDVVRQSLPMVLALLKMALVICIPLVLVVGTYDLKTVVTV
                     SVVQFALFFTDFWFQLARWIDSTILDALYGWGFGWNRPHTNFDPLVGLNNAFGDMLLM
                     FVTGTMFIVLPTFWIMALAWAGVRAGNVLQGLAGATGDAKAAGGRGGSIAINAVSKK"
     gene            complement(4104..4463)
                     /locus_tag="PKX01_16155"
     CDS             complement(4104..4463)
                     /locus_tag="PKX01_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3742 family protein"
                     /protein_id="HMZ77718.1"
                     /translation="MNTTTRTSTAERLGRAFGRGWRAYARCERRASNWLVSKGMPTAG
                     ATALVWMVKLAALGVLLYTTFWLALVMVCVMVVAWMARNTDLGEELPEPEWRNGPAGF
                     GLYTYDGFRIDPHVEDD"
     gene            complement(4548..>5136)
                     /locus_tag="PKX01_16160"
     CDS             complement(4548..>5136)
                     /locus_tag="PKX01_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HMZ77719.1"
                     /translation="LQHVSRVVYRGSPLYYGRSSTNRYDDPAQAYGVLYLGRDLPTAL
                     MESVFHKHQWLADTKRSIALKEVQSRMVRAVGVMDDVLLADLTAPGVMAGYFGLNLEQ
                     LASRDYTHTQQVSAQVHAMLGDDGQVLFDGVLYPSRNNYPAKSIALFERASAKVSVIE
                     DIDLVDHVDWPRFVATYRIGVEPDPGPLEPDDEAS"
BASE COUNT          882 a         1642 c         1714 g          898 t
ORIGIN      
        1 gggtggtggg tatctgctac tggctctact gcacctggac cggctgcacg gtgcgcacgt
       61 ccgtcaaggt caggcactac atccccgatg cggtggtttc gtcctactcg aacaccggcg
      121 agaacccctg ggtcgaagtg cgcgccatga gcacgcccaa cccttcggcc caggccggcg
      181 gggacggcac caccaatgaa gatcacgaaa acaatctcgc gaagttcaag aacgcggacg
      241 tcatcggcca ccccggcgtc gaggtgttca accagttcgt ctcgtcctcg ggctatttct
      301 gcgagggcgc aggcacggcc ttcatgccgt acctgctcag tacgctggac acgccggcct
      361 ggcgctacaa cgtaccggag atggtgtacc cggaggcatt gatcccgggc atgcgcgagg
      421 tcggcgcgcg ctcgaccctg aacctctggg gcaacgtcta tccgcgcggc ggcttcctgc
      481 accagacgga cgaccacaag gccggtgccg tggtggccca acgggccggc gatgtggtca
      541 cgcgccgcgg gcagatccac gtctatcagc cgctgctcgc caactcccgc gatggctact
      601 ggccggccgg cgcgctgatg gagggcgatg cctcgaccgg caagtggcag gaactcacgc
      661 ccgtcctgtc ctcgtcctgc acggtcttcc cgcgcaacgg cttcctgaca caggcccaac
      721 aaggcgacta cgcctgggcg ttgtggcggc cctatgcgtg ctgcgaacgc cggggccagg
      781 tgttcctcgg cagcgtcgat ttctattgag ggtacggcga tgaagcgtcc tgaactgatg
      841 aacccatccg ccaaggttcg ccgcccgctg cgccccacgg cgctagccgg cgcgctcgcc
      901 ctggtctgtg gcctcgcgtg ggcgcaggcc ggcttccaga ccagcggccc cgtcatcggc
      961 gatgaggtca tgtactcgat cggcggtggc agtgcggtgt ccatgggccg cgccgctggc
     1021 atgcgttcga tcggggtcgg cctgggttgg aacagcaacc tcatctgcgg cgacatgagc
     1081 atccagacca cgctgcgcaa tcagctcaat gggatcacga atgggttcca gcagatcatg
     1141 agcaacgtga tccagagcgc gaccagtgcc gtggcatcgc tgccggcgct gatcatccag
     1201 cgtgccgatc cggggctgta caacctgctg accaatggtg tgctgcaggc gaggctggat
     1261 ttcgaccgct ccaagctgac gtgtcgcgcg atggccgaga agatggccga gacggcgggc
     1321 ggccagctcg gatggagcca gatggcggaa ggcatggcgc tgcgcgatgc ggtgtcgagc
     1381 acggatgccg tatcggcgat cgagcaggcc gagacgcgcc gcggcaacga cggcgtgccc
     1441 tgggtgggcg gcagcaatgc cggcggcgcg ggccagtcgg ccatccgggt ggtcggtgac
     1501 gtgacgcggg cgggctacaa cctggtcaac gggcgcagcg tgactgacac gtcctccatc
     1561 gcgcccgcca gttgcgcgag cctgtcctgc cagacctgga cgtcgccgca gcaggcgacc
     1621 gaatgggcga cgcgggttct cggggaacag gtacagcgca cgtgtgattc ctgcaccaag
     1681 accgagaccg tgcctggcgt cgggctgacg ccgctgatcc aggaggagta cgagaccaag
     1741 ctggaggcct tgcaggaact ggtctctggg acgcgcaaca cgacgttcga gaacctgcat
     1801 gcagccggca gcacctcgct gcccattaca cgcggcgtca tcgaggcgct gcgcgacgag
     1861 ccagaccaag acctgctggc gcgacgtctc gcgtccgagg tcgcgttgtc gtccgtgctg
     1921 gagaaggcat tgctgctcca gcggaccctg ctgaccggca agaaggaacc caacgtggcg
     1981 gccaaccagt tggcggtcga ggccgtgaac cacgagagcg acacgctcga tcaggagatc
     2041 cgcaacctca agaccgaact tgaactgcgg cgcgaactgg cgaacaactc gcccatggcc
     2101 atcatccagc gccacgggac gcgcgcggcc ggctcgcgcg gcatctacga aggcgacccg
     2161 gtgcccgacc gcctcgatcg gttgcagcgg ggcaacccgg gaggcacgcc atgaacgcgg
     2221 cctggctgcg cccgcgctgg cttttcagcc ggcgcgcggc aaaggcgctg ctcctggcgg
     2281 tggtcatcat cgccgcggcc gtgggcgcca atctcgtcgg catctacctc gtcggcagcg
     2341 ttgccggctg ggagcggtgg ctggctgcca gcgcgggcta cttcctggtg tggcggctgt
     2401 gcctgtacgg cgcgacggtc tatggctggg tctggatgcg ccgccggctg ctggcccgcg
     2461 aggaagacgc gcaagcgcgg cgacgcctgg tgcgcagcga gatcgccggc gtcgtcgcca
     2521 tcgtggcgct ggaagccagc ctgctgatgc agagctgaag agggagccgc gccatgacgc
     2581 tttttacgac cgactacctg gagtactacc tgacgctggt gtcctggatc gtcaacaacg
     2641 gcatctgggc ggtgctggtg tcgagcggag tattcgcgct gccgttcgtc gccatcatcg
     2701 tgcaggagtg gttgaaggcc cgtgcggaag gtgccgacga aggcaacaag ggcgtgctct
     2761 cggctgcgcg catcgagaac cgggtcttcg tcgccatcgt ggtggtgatg ttcgctggca
     2821 tcccgttcat cgacgtggac ctcaacacca tccagtacga cagctcgcgc tcggcccagt
     2881 gccaggtcag cgtgccgcag cccacggata ccggctggtc gcagtccttc agcaccatca
     2941 acaaccagtc ggcgaaggtg cccgtctggt gggcgttcat gcacgcactc tcgcgcgccg
     3001 tcacgagcgc ctcggtggcg gcgatcccat gcggcacgga cctgcggcag atgcgcatgg
     3061 agatcgacgc gacccgcatt gacgacccgg tactggctca ggaagtagcg gatttctcgc
     3121 gggattgcta tgggcctgcg cgggccaaat tgttcatgca gcgccctcag cttgatgagc
     3181 agcagatgca cgacgtgacc tggatagggt cgaggttttt cacggacacg ggcggctact
     3241 acgacaccta ccgctcaagc acgccgcgcg acgactggcc ctacgacagc acccgcgatg
     3301 cggggcttgc acaggtgggc agcggtggcg gctacccgac ctgcaggcag tggtgggccg
     3361 atggcggcaa tggcctgcgg gcacgcctgc tgggacaggt ggacccgaac ctgttgaatc
     3421 gcctggcagg ctgggccggg ttcctgagca gggccgaggt ggacgactcg gtgatccgcg
     3481 ccatcgcgtc accgcggcaa cagaaattga accagggtag cgtctatacg gactatggcg
     3541 gccagatcga caagaccttg ccgaacatcg tgacgcgggc cacgggagac gttgggatgg
     3601 ccgtcggcgc gattgccgcg tttcccgcca tggacgtcgt gagacagtct ctgcccatgg
     3661 tcctcgcctt gctcaagatg gcgctggtca tctgcatccc gcttgtgctg gtcgtaggca
     3721 cctacgatct gaagacggtc gttaccgtca gcgtcgtgca gttcgcgctc ttcttcacgg
     3781 acttctggtt tcagcttgcg cgctggatcg attcgacgat cctggatgcg ctctatggct
     3841 gggggttcgg ctggaatcgg ccgcacacca acttcgaccc gctggtgggg ctgaacaacg
     3901 ccttcggaga catgctcctg atgtttgtca cgggcacgat gttcatcgta ctgcctacgt
     3961 tctggatcat ggccttggct tgggcgggcg ttcgtgccgg caacgtgttg caaggcctcg
     4021 caggagccac cggagatgcc aaggctgctg gcggaagggg aggaagcatt gcgatcaatg
     4081 cagtgtcgaa gaagtgatcg ctgctagtcg tcctcgacat gaggatcaat gcggaaaccg
     4141 tcgtaggtgt ataggccgaa tcctgctggt ccgtttcgcc actctggctc gggcaactcc
     4201 tcgcccaagt cggtgttgcg ggccatccag gcaaccacca tgacgcacac catcaccagg
     4261 gccagccaga aggtggtgta cagcagcacc ccgagcgcag ccagcttaac catccacacg
     4321 agcgcggtgg ctccagccgt cggcatgccc ttggaaacca accagttcga cgcccgccgt
     4381 tcacagcgcg cgtaggcacg ccatccgcgg ccaaaggcgc ggccgaggcg ttctgcggtg
     4441 ctggtgcgag tcgtggtgtt catggtcgtc tcctactgct gagggatgcc tatttcagtt
     4501 tggtccattt cattctgttt agggcttcaa tgtgttctct tgctgcttca ggacgcttcg
     4561 tcatccggtt ccaacgggcc agggtcgggc tccacgccga tgcggtatgt agcaacgaaa
     4621 cgcggccagt ccacatggtc aacgagatcg atgtcctcga tgacactgac cttcgcgctg
     4681 gcacgctcga acagcgcgat gctcttggcg ggatagttgt tgcgcgacgg atagagcacc
     4741 ccgtcgaaca gcacctggcc gtcatctcca agcatcgcat gcacctgggc cgacacctgc
     4801 tgcgtgtgcg tgtagtcgcg gctggccaac tgctccaggt tcaggccgaa gtagcccgcc
     4861 atgacgcccg gcgccgtgag gtcggccaga agcacgtcgt ccatcacgcc cactgcgcgc
     4921 accatccggc tctggacttc tttcagcgcg atcgagcgct tcgtgtccgc aagccactgg
     4981 tgcttatgaa acaccgactc catcagcgcc gtgggcaggt cgcgccccag gtagagcacc
     5041 ccgtaggcct gggccgggtc atcgtagcga ttggtgctgc tgcggccata gtacagcggg
     5101 ctgccccgat agacgacgcg gctcacatgc tggagc
//
LOCUS       DAOQPA010000178         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10409_length_5004_cov_18.833300, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000178 DAOQPA010000000
VERSION     DAOQPA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10409_length_5004_cov_18.833300"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..915
                     /locus_tag="PKX01_16165"
     CDS             544..915
                     /locus_tag="PKX01_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77720.1"
                     /translation="MPKTQVGRVQPNVLQADVDAVRGLKMIHDYHPTNPDYSLEAVAA
                     LLQRMLDAEEAEVQAQATLSAARDATVAAQWAFHTSVLGVKGQIKALYGEDSDQVATI
                     GLKKKSDRKSPSKSTKPPTTT"
     gene            1087..1368
                     /locus_tag="PKX01_16170"
     CDS             1087..1368
                     /locus_tag="PKX01_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77721.1"
                     /translation="MKNTTAIFALLLAGCASTGVVPVAHDEYTISKQSAAGMLGSPNG
                     VKADIYDEAHAFCTQQGKDVETVALDLKHAIPFVQPGSASLKFKCVAKS"
     gene            complement(1511..2128)
                     /locus_tag="PKX01_16175"
     CDS             complement(1511..2128)
                     /locus_tag="PKX01_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ77722.1"
                     /translation="MKFYMTPGSCSTGIHILLEEVGAVFEVYIVNLPAGDHLKADYVA
                     LNPKSTIPVLVRKDGTALTEFQAIAWWLARQYPKRSLLPDDAEGEVRVLEVMDYVVGT
                     IHGQGFTRIFTTPNFTPSPADHPVVQARGREIVEQGFAVLDATLAGRDYVTGRFSIAD
                     AALFYTEFWADRLKMDLPPNCRAHYQRMLERPAVRQVLREEGYRV"
     gene            complement(2316..3395)
                     /locus_tag="PKX01_16180"
     CDS             complement(2316..3395)
                     /locus_tag="PKX01_16180"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMZ77723.1"
                     /translation="MPTIKVMIVDDSAVVRQVLSSTLDEDAAIEVIGTAVDPVFALER
                     MKSRWPDVIVLDVEMPRMDGITFLRKIMAERPTPVVICSTLTEKGSETALQALSAGAV
                     CVVTKPKLGLKQFLQDSSDDLVSAVKAASKANVRRLAAAARPAPPPAPPVHSADVILP
                     AGQAMVQTTETVIAIGTSTGGTQALEAVLRSLPRMSPGIVVVQHMPEKFTEAFAQRLD
                     SLCEVDVREARHGDRVVPGRVLIAPGGRHMLLRRSGAQYVVDVVDGPPVSRHRPSVDV
                     LFRSTARFAGRNAVGIIMTGMGDDGARGLKEMHEAGAWTVAQDEATCVVYGMPKEAVR
                     LGAVDKVLGLHDIPAAILGRISRKG"
     gene            complement(3412..3915)
                     /locus_tag="PKX01_16185"
     CDS             complement(3412..3915)
                     /locus_tag="PKX01_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HMZ77724.1"
                     /translation="MRKPDGVVEIFLQPGEWYFGDRYTRIRTVLGSCVSAVFWHSGLL
                     MGGMCHFVLPSRGRLPAAADLDGRYGDEAFRLMVRELRGSGGRPDDFQVRLFGGANMF
                     PAIARGSRGPLVGQKNVDAARALVRDHGLKCVGAHVEGEGHRHLLFDVWSGRVTLKGA
                     HGDTSSP"
     gene            complement(3920..4765)
                     /locus_tag="PKX01_16190"
     CDS             complement(3920..4765)
                     /locus_tag="PKX01_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HMZ77725.1"
                     /translation="MTAPASHASAPTAGGPGPLAEREFHLFRTLMHRHAGISLSDAKR
                     QLVAGRLAKRLRQLELDSYASYFRLLERNTAERQTAVDLLTTNETYFFREPKHFDFLR
                     EHILPELKGHSPLRVWSAACSSGEEPFTLAMVMAETLGNRPWEIVASDLSTRVLEAAR
                     EGRYTLADAEGIPRPLLVRYGLRGVGSQEGSFLFKPDLRKRIQFRQINLNTVLPDLGL
                     FDVIFLRNVMIYFDMDTKVQVVKRVLAQLRPGGYLFLSHSENLNGVSDAVKSVRPSVY
                     RKPEA"
     gene            complement(4790..>5004)
                     /locus_tag="PKX01_16195"
     CDS             complement(4790..>5004)
                     /locus_tag="PKX01_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ77726.1"
                     /translation="SEELAATAEEMSAQAELLTRTMSYFKLAVAERRSSTELLRPSRN
                     GRSDADGSRPARRAPVVESEYVEFAA"
BASE COUNT          773 a         1655 c         1821 g          755 t
ORIGIN      
        1 ggttcgccct gatcggccgc cgttagcctg actccccgac cacaaaaagc acccgccaga
       61 tcacccggcc tgcctttcgc cagcgcaccc cagcctcagc ccggacctct tcccgtctgc
      121 aggcgccgag cagcacagcg gcaggcgggg atgtccggcg cagctgtctg agcccgcagg
      181 gcgagttctg cgccggccgc ctgacgcgcg cagcgcaggg gagtcggcca aaggccgacc
      241 gccggaagcg ggtcgccttc tttgcccact ttcttggcga agcaagaaag tgggtcgccc
      301 gccggggcga actcccggcc aacgatacgc cacacccgcg ccgaccgtga tccgccgccg
      361 ccgggccaca gccctctggc cagccgcgtg ccacgccccc gccggccacg cccaccgcat
      421 acgctcctgc cataaatcca aattccgcgc aacgtcatgc ttcactgaca acaccgatcc
      481 gatgggcata cgatgcgcct gccgtagatg cctgcggcat tccacctact gaaggagact
      541 ttcatgccca agactcaggt tggccgcgtt cagcccaacg tcctgcaagc cgatgtggat
      601 gccgtgcgcg gcctcaagat gatccacgac tatcacccca ccaacccgga ctacagcctg
      661 gaggccgtcg cggcgcttct ccagcgcatg ctcgacgcgg aggaggccga ggtgcaggcc
      721 caggcgacct tgtccgccgc ccgcgatgcc acggtggcgg cccagtgggc cttccacacg
      781 agcgtgctcg gggtgaaggg ccagatcaag gcgctctacg gggaggattc cgaccaggtg
      841 gcgaccatcg gcctcaagaa gaagtccgac cgcaagtcgc cgagcaagag caccaagccg
      901 cccacgacga cctgagggca tcacggcggg acgggggccg ggcacatccg cgcgggtggc
      961 ccatccgccc cgctcacacc ggcgcccccg cccagaacac tcgattccgc caggaacgca
     1021 agggtattcc gcaacaccgt gccgatggga cagggcacgg tctaagccgc accatcaagg
     1081 accaacatga agaacacgac cgccattttc gcgctgctgc tggcaggctg cgcatcgacc
     1141 ggggtggtcc cggtcgccca cgatgaatac acgatctcga agcagagtgc cgccggcatg
     1201 ctgggctccc cgaacggggt caaggccgac atctatgacg aggcccatgc gttctgcacg
     1261 cagcagggca aggacgtcga gacggtcgcg ctcgatctga agcacgccat tccttttgtc
     1321 cagccgggca gcgcttccct gaagttcaag tgcgtcgcga aaagctgatg gacggcgtgc
     1381 actggcgcgc ggtgctgccc gcacggggcg ccccgcgcca gccttgaggg cgcaccgtac
     1441 cggctgcccg gccggcgcgg ccctggatgc cgtcggtggc gggacgtctg cccgcctcgc
     1501 gccccccttc ctagacccga tacccctcct cccgcagcac ctggcgcacg gccgggcgtt
     1561 cgagcatgcg ctggtagtgg gcgcggcaat tcggcggcag gtccatcttg agacggtcgg
     1621 cccagaattc ggtgtagaac agggccgcgt cggcgatgga gaagcggccg gtcacgtagt
     1681 cccgcccggc cagggtggcg tcgagcacgg cgaagccctg ttcgacgatc tcgcggccgc
     1741 gggcctgcac gacggggtgg tcggccgggc tgggggtgaa gttgggcgtg gtgaagatgc
     1801 gggtgaagcc ctggccgtgg atggtgccga ccacgtagtc catgacttcc agcacccgca
     1861 cttcgccttc ggcgtcgtcg ggcagcaaac tgcgtttggg gtactggcgg gccagccacc
     1921 aggcgatggc ctggaattcg gtgagggcgg tgccgtcctt gcggaccagg acggggatgg
     1981 tggatttggg gttgagggcc acgtagtcgg ccttgaggtg atcgccggca ggcaggttga
     2041 cgatgtacac ctcgaagacg gcgcccactt cctccagcag gatgtggatg ccggtggagc
     2101 aggaaccggg ggtcatgtag aacttcacgg gggtggcctc ggcagggaag atgcccctgg
     2161 caagcaagag cgggaccacg cccgcgccgc ggcgggcctg tgccgcccgg gccggcgggc
     2221 gtccgcgctg gatgcgggcg gtcgcatctg ccacagggcg tgtcggcttt gcgccacggg
     2281 cgcgccgggc gctccgcccc gccgggcggc gatcttcagc ccttgcggct gatgcggccg
     2341 aggatcgcgg cggggatgtc gtgcaggccc agcaccttgt ccacggcgcc gaggcgcacg
     2401 gcctccttgg gcatgccgta gacgacgcag gtggcttcgt cctgggccac ggtccacgcg
     2461 ccggcctcgt gcatctcctt gagcccgcgg gcgccgtcgt cgcccatgcc ggtcatgatg
     2521 atgcccaccg cgttgcgtcc ggcgaagcgg gcggtagagc ggaacagcac gtccaccgag
     2581 gggcgatggc ggctcaccgg ggggccgtcc accacgtcca cgacgtactg cgcgccgctg
     2641 cggcgcagga gcatgtggcg gccgccgggg gcgatgagga cgcggccggg aacgacccgg
     2701 tcgccgtggc gggcttcgcg cacgtccacc tcgcacaggc tgtcgaggcg ctgggcgaag
     2761 gcttcggtga acttttcggg catgtgctgg accaccacga tgcccggcga catgcgcggc
     2821 agggaccgca gcacggcttc gagggcctgg gtgccgccgg tggaggtgcc gatggcgatc
     2881 actgtctcgg tggtctggac catggcctga ccggcgggga ggatcacgtc ggcggaatgg
     2941 acgggcggcg cgggcggggg cgccggccgc gcggcggcgg cgaggcggcg cacgttggcc
     3001 ttggacgcgg ccttgacggc gctcaccagg tcgtcggagc tgtcctgcag gaactgcttg
     3061 aggcccagct tgggcttggt gacgacgcac acggcgccgg cggagagggc ctgcagggcg
     3121 gtctcggagc ccttttcggt gagggtggag cagatcacga cgggcgtggg gcgctcggcc
     3181 atgatcttgc gcaggaaggt gatgccgtcc atgcggggca tctccacgtc gagcacgatc
     3241 acgtcgggcc accgggattt catgcgttcc agggcgaaga ccgggtccac ggcggttccg
     3301 atcacttcga tggcggcgtc ttcgtccagg gtggaggaca gcacctggcg gaccacggcg
     3361 gagtcatcca cgatcatcac tttgatggtg ggcattgttc ttgtctctct gtcaggggct
     3421 ggaggtgtcg ccgtgggcgc ccttgagggt cacccgcccg ctccacacgt cgaagagcag
     3481 gtggcggtgg ccctcacctt ccacgtgggc gccgacgcat ttgaggccat ggtcacggac
     3541 cagggcgcgg gcggcgtcca cgttcttctg gcccaccagg ggcccgcggc tgccgcgggc
     3601 gatggcggga aacatgttgg cgccgccgaa gaggcgcacc tgaaagtcgt ccgggcgccc
     3661 gccgctgccg cgcagctcgc gcaccatgag gcggaaggct tcgtcgccgt agcggccgtc
     3721 caggtcggcg gcggcgggca ggcggccgcg gctgggcagc acgaagtggc acatgccgcc
     3781 catcagcagg ccggaatgcc agaacacggc cgagacgcag gagccgagca cggtgcggat
     3841 gcgggtgtag cggtcgccga aataccattc ccccggttgc aggaagatct cgacgacgcc
     3901 gtcgggtttg cgcatggcct catgcctcgg gtttgcggta aacggacgga cgcacgctct
     3961 tgaccgcgtc gctgaccccg ttgaggtttt ccgagtggct gaggaagagg tagccgcccg
     4021 gccgcagctg ggccagcacc cgcttgacca cctggacctt ggtgtccatg tcgaaataga
     4081 tcatgacgtt gcgcaggaag atgacgtcga agaggcccag gtccggcagc acggtgttga
     4141 ggttgatctg gcggaactgg atgcgcttgc gcagatcggg cttgaacagg aagctgcctt
     4201 cctggctgcc cacgccgcgc aggccgtagc gcaccagcag cggacgggga atgccctcgg
     4261 cgtcggcgag ggtgtagcgc ccttcccgcg ccgcttccag cacgcgggtg gagagatccg
     4321 aggcgacgat ctcccacggg cggttgccga gggtctcggc catcaccatg gcgagggtga
     4381 agggttcctc gccgctggag caggcagcgc tccacacgcg gagggggctg tggcccttca
     4441 gttccggcag gatgtgctcg cgcaggaagt cgaagtgctt gggctcgcgg aagaagtagg
     4501 tctcgttggt ggtgagcagg tccaccgcgg tctggcgctc cgcggtgttg cgctccagca
     4561 ggcggaagta gctggcgtag ctgtccagct ccagctgacg caggcgcttg gccaagcggc
     4621 cggccaccag ctgccgcttg gcgtcggaca ggctgatgcc ggcgtggcga tgcatcaggg
     4681 tgcggaacag gtggaattcc cgttccgcca gggggccggg gcccccggcg gtgggtgcgg
     4741 aggcatgcga tgccggggca gtcatggggg cgatctccag tccgggggct caggccgcga
     4801 attccacgta ttcgctctcg accaccgggg cgcgccgcgc cgggcggctg ccgtccgcgt
     4861 cgctgcgccc atttcgggac ggccgcagga gctcggtgct gctgcgccgc tcggcgaccg
     4921 ccagcttgaa gtaggacatg gtgcgggtga gcagttcggc ctgggcgctc atctcctcgg
     4981 cggtggcggc gagctcttcg gagg
//
LOCUS       DAOQPA010000179         4939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10605_length_4939_cov_21.760238, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000179 DAOQPA010000000
VERSION     DAOQPA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_4939_cov_21.760238"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="soxC"
                     /locus_tag="PKX01_16200"
     CDS             <1..819
                     /gene="soxC"
                     /locus_tag="PKX01_16200"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HMZ77727.1"
                     /translation="CGANTGMEWGNVAVPTVQYTHGMLSCSEFTGVPLRTLLDDCGVN
                     WKKARYVLAEGADGSSMTRTIPIELVESGEVLVAYGQNGEMLRPENGYPLRLVVPGVQ
                     GVSWVKWLRRIEVGDQPWATKDEAIHYVDLLPGGQHRQYTSIQECKSVITTPSGGQML
                     LDKGFYNISGLAWSGRGKIARVDVSTDGGVNWRSARIDGPVHGKCLTRFNIDWVWDGR
                     PAILQSRAVDDTGYVQPRYGQLRAVRGTKSIYHNNAIQSWKVAETGEVSNVQVL"
     gene            803..1879
                     /locus_tag="PKX01_16205"
     CDS             803..1879
                     /locus_tag="PKX01_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ77728.1"
                     /translation="MSRFSRIPLALVALVALGLAGTAVADGKFPGIGRPATAAEVRAW
                     DIDVRPDFKGLRPGSGSVDQGQEVWEAKCASCHGTFGESNEVHTPLIGGTTQDDIRTG
                     HVASLAKGDVPQRTAIMKVATVSTLFDYINRAMPLTAPKSLKNDEVYALLAYLLNLAE
                     VVPADFVLSDRNIAEVQQRMPNRKGMTTDHGMWPGASAARGGIGNGGTPDVKNTACMH
                     RCKDAVQVASLLPAYAETAHGNLAEQNRSVGPVRGKETGARAMADAKSVALSENAAMA
                     KLAGDSGCMACHGLASRVVGPGYNEVATRYTGQPDAEAKLAAKVKAGGQGVWGAIPMP
                     PQSQLKDGDVRSLVKWILSGAKAQ"
     gene            1908..2375
                     /gene="soxY"
                     /locus_tag="PKX01_16210"
     CDS             1908..2375
                     /gene="soxY"
                     /locus_tag="PKX01_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HMZ77729.1"
                     /translation="MNMERREVLKAGSGLGLLGLLVAAGLITPGEARAEWAKAAFDAK
                     SIEDTLKALGAGKPDSSADIQITAPDIAENGAVVPVGVVTALPDVQVIALLVEKNPNP
                     LAASFTLPAGTEPSVQTRVKMGQTSDVYAVVKAGGKFLMTKKEIKVTLGGCGG"
     gene            2398..2709
                     /gene="soxZ"
                     /locus_tag="PKX01_16215"
     CDS             2398..2709
                     /gene="soxZ"
                     /locus_tag="PKX01_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HMZ77730.1"
                     /translation="MADPMKIRASAKDGMTEVKVLMSHEMETGQRKDASGALVPAHFI
                     TEVTAEHNGRPVLAAQFGTAVSKNPYLAFKFKGGAKGDKLTVSWVDSKGDTRRDEVLI
                     A"
     gene            2790..3596
                     /gene="soxA"
                     /locus_tag="PKX01_16220"
     CDS             2790..3596
                     /gene="soxA"
                     /locus_tag="PKX01_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HMZ77731.1"
                     /translation="MRGFMRTALATAAVVLSAPVWAEGSTQDEIARYREMLAEGNPAE
                     LLEAKGEELWKKPRGPRNVSLERCDLGLGPGQVQGAYARLPRYFADTRQVMDVESRLV
                     HCMVTLQGFTREEATRNWYSRPGKDSDIEALVTYIGGQSNGVAIDVPARHPEEARMAR
                     IGELIFYRRSGPQDFSCAICHGQEGKRIRLQELGKLTTRAGAGTAMANWPSYRVSQGA
                     VWTMQRRLIDCMRQARWPEPEYLSDSVIALETFLQKTASGTVMQTPGIKR"
     gene            3607..4266
                     /gene="soxX"
                     /locus_tag="PKX01_16225"
     CDS             3607..4266
                     /gene="soxX"
                     /locus_tag="PKX01_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HMZ77732.1"
                     /translation="MKTFAFTAGLAAILAGAVTAPHAQAADRTASADKTLAAQAEQVF
                     RSGFRRDNPPYWMARIEQDEAMRLCSQYKDDPPDAVRRRIEAAQRATLRYPVDGKLVG
                     DWREGEKLASSGRGGHIGFIQPDPPGTPRGGNCYACHELAKKEVAYGTIGPSLRHFGR
                     LRGGGDDVVRYAYEKIYNSNIFMACTNMPRFGLHSWLTPEQITHLVAFLIDPESPVNK
                     D"
     gene            4280..>4939
                     /locus_tag="PKX01_16230"
     CDS             4280..>4939
                     /locus_tag="PKX01_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HMZ77733.1"
                     /translation="MSMTRRDFLQVLAVAAAGGMALHSHPARAAKAAERLYDLPRFGS
                     VSLLHMTDCHAQLLPIHFREPDVNLGVGPMAGQVPHLVGEHLLRHFGIRPGTPEAHAF
                     SSLDFVKAARTYGKVGGFAHLATLVKRLKASRPGALLLDGGDTWQGSGLSLWTKAQDM
                     VEACKALGVDVMTLHWEATYGEARVKEIEEKDFAGHIDIVAQNIKTADFGDPVFKPYV
                     IR"
BASE COUNT          888 a         1686 c         1660 g          705 t
ORIGIN      
        1 tgcggtgcca acaccggcat ggagtggggc aacgtggcgg tgcccacggt gcagtacacc
       61 cacggcatgc tgtcctgttc agaattcacc ggcgtgcccc tgcgcacgct gctggacgac
      121 tgcggggtga actggaagaa ggcgcgttac gtgctcgccg aaggcgccga cggttcgtcc
      181 atgacccgca cgatcccgat cgagctcgtg gagtccggcg aggtgctggt ggcctacggt
      241 cagaacggcg agatgctgcg ccccgagaac ggctacccgc tgcgcctggt ggtacccggc
      301 gtgcaggggg tgagctgggt caaatggctg cgccgcatcg aggtgggcga ccagccctgg
      361 gcgaccaagg acgaggccat ccactacgtg gacctgctgc ccggcggcca gcatcgccaa
      421 tacacctcca tccaggaatg caagtcggtc atcacgaccc cgtccggggg ccagatgctg
      481 ctggacaagg gcttctacaa catctccggg ctcgcctggt ccggccgcgg caagatcgcc
      541 cgggtggatg tgtccaccga tggcggcgtc aattggcggt cggcgcgcat cgacgggccc
      601 gtgcacggca agtgcctgac ccgcttcaat atcgactggg tgtgggacgg gcgaccggcg
      661 atcctccagt cccgcgcggt ggacgacacc ggctatgtgc agccccgcta cgggcagctg
      721 cgcgccgtgc gcgggaccaa atcgatctat cacaacaacg cgatccagtc gtggaaggtg
      781 gcggaaacgg gggaggtgag caatgtccag gttctctagg attccactgg cgctggttgc
      841 cctggtcgcc ctggggctgg cggggacggc cgtggcggac gggaagttcc cgggcattgg
      901 tcggccggcc acggcggccg aggtgcgtgc ctgggacatc gacgtgcgtc ccgatttcaa
      961 gggcctgcgc cccggcagcg gcagcgtgga ccaggggcag gaggtctggg aggccaaatg
     1021 cgcgtcctgt catggcacct tcggagagtc caacgaggtg catacgccgc tcatcggcgg
     1081 caccacacag gacgacattc ggaccggtca cgtggcttcc ctggcgaagg gtgacgtgcc
     1141 ccagcgcacc gccatcatga aggtggcgac ggtgtccacc ctgtttgact acatcaaccg
     1201 ggcgatgccc ctgactgccc ccaagagcct caagaatgac gaggtctatg ccctgctcgc
     1261 ctacctgctc aatctggccg aggtggtgcc cgccgacttc gtgctgagcg accggaacat
     1321 cgccgaggtg cagcagcgca tgcccaaccg caagggcatg accaccgatc acgggatgtg
     1381 gcccggtgcc tcggcggcta ggggaggcat cggcaacggc ggcacgccgg acgtgaagaa
     1441 caccgcctgc atgcatcgct gcaaggacgc ggtgcaggtg gcgtccctgc tgccggccta
     1501 tgccgagacg gcccacggca atctggcgga gcagaatcgc agcgtcggcc cagtgcgtgg
     1561 caaggagact ggcgcgcggg ccatggcgga cgcgaaatct gtggcgctga gcgagaatgc
     1621 ggcgatggcg aaactcgccg gtgacagcgg ctgcatggcc tgccacggtc tggccagccg
     1681 cgtcgtcggg cccggttaca acgaagtggc cacccgctac acgggccagc ccgacgccga
     1741 ggcgaagctg gcggccaagg tcaaggccgg cgggcagggt gtctggggcg cgatccccat
     1801 gccgccccag agccagttga aggacgggga tgtgcgcagc ctcgtgaagt ggatcctgtc
     1861 cggcgccaag gcgcaataaa cgacatcaac gaaggagaga gagacctatg aacatggaac
     1921 gccgtgaagt cctcaaggcg ggcagcgggc ttggcctgct cggcctgctg gtcgccgccg
     1981 gactgatcac cccgggcgag gcccgcgccg aatgggccaa ggcggccttc gacgcgaaga
     2041 gcatcgaaga caccctcaag gccctgggcg ccggcaagcc ggacagctcg gccgacatcc
     2101 agatcaccgc gccggacatc gccgagaacg gcgccgtggt gccggtgggc gtggtcaccg
     2161 cgctgcccga cgtgcaggtc atcgccttgc tggtggagaa gaatcccaat ccgctggcgg
     2221 ccagcttcac gctgccggcc ggcaccgagc cgagcgtgca gacccgcgtc aagatgggac
     2281 agacctccga cgtctacgcc gtggtcaagg ccggcggcaa gttcctcatg accaagaagg
     2341 aaatcaaggt caccctcggc ggctgcggcg gctgatcaaa cattccagga gattcccatg
     2401 gcagacccga tgaagatccg cgccagcgcc aaggacggca tgaccgaagt gaaggtgctg
     2461 atgagccacg agatggagac cggccagcgc aaggacgcct ccggcgccct ggtgcccgct
     2521 catttcatca ccgaagtgac cgccgagcac aacggccggc cggtgctggc ggcccagttc
     2581 ggcaccgccg tgtcgaagaa cccctacctc gccttcaagt tcaagggcgg ggccaagggc
     2641 gacaagctga cagtgagctg ggtggatagc aagggcgaca cgcgtcgcga cgaagtcctg
     2701 atcgcctgac gcgccggggc gggcgcatcc cgcccatgcc acaagaccgc cggtcaggcg
     2761 gcacccccac acacgaggag gaagagacga tgcgtggatt catgcggacg gcgctcgcca
     2821 ccgcggccgt ggtcctgagt gcgccggtct gggccgaggg ctccacccag gatgagatcg
     2881 cccgttaccg ggaaatgctc gccgagggca acccggccga gctgctggaa gccaagggcg
     2941 aggaactctg gaagaaaccc cgcggccccc ggaacgtcag tctggaacgc tgcgacctgg
     3001 gcctcggacc ggggcaggtc cagggcgctt acgcccgcct gccgcgctac ttcgcggaca
     3061 cccgtcaggt catggacgtg gagtcgcgtc tggttcactg catggtgacg ctgcagggct
     3121 tcacccgcga ggaggcgacg cgcaactggt attcgcggcc cggcaaggat tcggacatcg
     3181 aggctctggt gacctacatc ggcggccagt ccaacggcgt cgccatcgac gtgccggcgc
     3241 gccacccgga agaggcccgc atggccagga tcggcgagct gatcttctat cgccgcagcg
     3301 gcccgcagga cttctcctgc gccatctgcc acggccagga aggcaagcgc atccgcctgc
     3361 aggaactggg caagctcacc acccgcgcgg gagccggcac ggccatggcc aactggccgt
     3421 cctaccgggt ctcccagggc gctgtctgga ccatgcagcg ccgcctcatc gactgcatgc
     3481 gtcaggcccg ctggcccgag ccggaatatc tgtccgactc cgtcatcgcc ctggagacct
     3541 tcctgcagaa gaccgccagc ggcaccgtga tgcagacccc cggaatcaag cgctgaggcc
     3601 ccgaccatga agaccttcgc attcaccgcc gggctcgccg cgatcctggc gggcgctgtc
     3661 actgcgcccc atgcccaggc ggccgaccgg accgcgtcag ccgacaagac cctcgccgcc
     3721 caggccgagc aggtctttcg cagtggcttc cggcgcgaca atccgcccta ctggatggcc
     3781 cgcatcgaac aggacgaggc catgcgcctg tgcagccagt acaaggacga cccacccgac
     3841 gccgtgcgcc gccgcatcga agccgcccaa cgcgccaccc tgcgctaccc ggtggacggc
     3901 aagctcgtcg gcgactggcg cgagggcgag aagctcgcct ccagcggtcg tggcggccac
     3961 atcggcttca tccagcccga tccgccgggc acgccgcggg gcggcaactg ctatgcctgc
     4021 cacgagctgg ccaagaagga agtcgcctac ggcaccatcg gccccagcct gcgccatttc
     4081 gggcgcctgc gcggcggcgg tgacgatgtg gtgcgctatg cctacgagaa gatctacaac
     4141 tccaacatct tcatggcgtg caccaacatg ccccgtttcg gcctgcacag ctggctcacg
     4201 ccggagcaga tcacccacct cgtcgcattt ctgatcgatc cggaatctcc ggtgaacaag
     4261 gactgacagg agaccgccca tgtccatgac ccgtcgggat tttctccagg tgctcgctgt
     4321 cgccgccgcg ggcgggatgg ccttgcacag tcatcccgcc cgcgcggcga aggccgccga
     4381 gcgcctgtac gatctgcccc gcttcggcag tgtcagcctg ctgcacatga cggactgcca
     4441 tgcccagctg ctgcccatcc actttcgcga gcccgacgta aacctgggcg tcggccccat
     4501 ggccgggcag gtgccgcacc tggtgggcga acacctgctg cgccatttcg gcatccgccc
     4561 cggcacgccg gaggcccacg ccttcagttc gcttgacttc gtgaaggccg cccgcaccta
     4621 cggcaaggtc ggcggtttcg cccatctcgc caccctcgtc aagcgcctca aggcgagccg
     4681 gcccggcgcg ctgctgctgg atgggggaga cacctggcag ggctccggcc tcagcctgtg
     4741 gacgaaggcc caggacatgg tggaggcctg caaggccctc ggtgtagacg tgatgaccct
     4801 gcactgggaa gccacctacg gtgaagcccg cgtcaaggag atcgaggaaa aggacttcgc
     4861 cggccacatc gacatcgtgg cccagaacat caagaccgcc gacttcggcg acccggtgtt
     4921 caagccctac gtgatccgc
//
LOCUS       DAOQPA010000180         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10677_length_4917_cov_17.636364, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000180 DAOQPA010000000
VERSION     DAOQPA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10677_length_4917_cov_17.636364"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKX01_16235"
     CDS             complement(<1..706)
                     /locus_tag="PKX01_16235"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HMZ77734.1"
                     /translation="MSISRHPLANSLRASDTGKIVSAREAVNLIRDGDTVATGGFVGI
                     GFPENIAIALEELYLENQGSEGQGLGFPRNLTLVYAAGQGDGKDRGLNHLGHDGLVSR
                     VIGGHWGLVPKLQQLAVANRIEAYNLPQGVITHLFRDIAAGKPGTLTRVGLGTFVDPR
                     FGGGKLNEKTTADVVRLMQIDGEDYLFYKAFPINVGIIRGTTADPDGNITMEKEALTL
                     EAQAIAMAAHNSGGIVI"
     gene            complement(743..2143)
                     /locus_tag="PKX01_16240"
     CDS             complement(743..2143)
                     /locus_tag="PKX01_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HMZ77735.1"
                     /translation="MSFLIVLAALCFLMFVAYRGYSVILFAPVAALGAVLLTDPTLVA
                     PMFTGLFMDKMVGFVKNYFPVFLLGAIFGKVIELSGFSKSIVSSVIKVVGAERAMLSI
                     VLVCSLLTYGGVSLFVVVFAVYPFAAEMFRQGGIPKRLIPGTIALGAFTFTMDSLPGT
                     PQIQNVIPTTFFKTNIYAAPVLGTVGAVFIFIVGLSYLEWRRRTAKSAGEGYGTELLN
                     EPEPFDETEKLANPVLAILPLVLVGVTNKLFTDLIPQIYGTSHSFVPAVIGTAKPVVQ
                     DVSKIAAIWAVEGALLVGILTVMVFAWRTVSTKFAEGTKAAVGGALLASMNTASEYGF
                     GAVIAALPGFLVVADALKVIPNPLVNEAITVTTLAGITGSASGGMGIALAAMSETFIQ
                     AANAAGIPLEVLHRVASMASGGMDTLPHNGAVITLLAVTGLTHKVAYKDIFAITMIKT
                     TAVFVIIATYYATGLV"
     gene            complement(2301..3719)
                     /locus_tag="PKX01_16245"
     CDS             complement(2301..3719)
                     /locus_tag="PKX01_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ77736.1"
                     /translation="MSPEHFLADAERIRQRVIDTLLAEFAELSEGVVIVDRDARIVWM
                     NDKYPRHLMVHDAADIIGRPVEEVIPNSLMRQVVSTGKPIMLDIMELGHESVVVMRLP
                     VRDEHGEVIGGVGMVIFDDARQLAPVVSRFQSLRTELADTRRKLDEARRSKYTFASFI
                     GASPRCVAVKNKARRAARAASPVLILGETGTGKELLAHAIHAASPRAGGPFVAVNIAA
                     VPETLLEAEFFGVAPGAYTGAERKGRSGKFELAQGGTLFLDEIGDMSPALQSKLLRAL
                     QEKEIEPVGSNRLISVDVRIIAATSRDLPKEIEAGRFRADLFYRLNVITLDVPPLRDR
                     LDDLPFIAEALTDSLARQLDEPPRSLSRAALDYLAHHSWPGNVRELANVLERALLMSD
                     ADCLDQADFEQVLPGLVRPPARPVAENARTLDQIQQATERQAIADAIAAAGGNKAQAA
                     RSLGISRASLYEKIAALGIAAP"
     gene            complement(3904..>4917)
                     /locus_tag="PKX01_16250"
     CDS             complement(3904..>4917)
                     /locus_tag="PKX01_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HMZ77737.1"
                     /translation="LHKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHE
                     LKAKAASGAPLSRAEALRLELMEKINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPI
                     ALVPNCAATRHCHFHLDGSGPAKLDPPKLEDWPAVTWTPDVKSATRVNLDTLTKEEVA
                     SWKVGQKILLNGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVV
                     GPAGPTTATRMDKFTRMMLEQTGLISMIGKSERGPVAIEAIKDNNSAYLMAVGGAAYL
                     VAKAIKSAKVVGFEDLGMEAIYEFDVQDMPVTVAVDSTGTSVHNTGPKEWQVKIGKIP
                     VKA"
BASE COUNT          754 a         1464 c         1842 g          857 t
ORIGIN      
        1 cgatgacgat gccgccggag ttgtgggccg ccatggcgat ggcctgggcc tccagagtga
       61 gggcctcctt ctccatggtg atgttgccgt ccgggtcggc ggtggtgcca cggatgatgc
      121 ccacgttgat cgggaaggcc ttgtagaaca gatagtcctc gccgtcgatc tgcatcaggc
      181 ggaccacgtc ggcggtggtc ttctcgttga gcttgccgcc gccgaagcgt gggtctacga
      241 aggtaccgag gccgacccgc gtcagcgtgc cgggcttgcc ggcggcgatg tcgcggaaca
      301 ggtgggtgat gacgccctgg ggcaggttgt aggcttcgat gcggttggcc acggcaagct
      361 gctgcagctt gggcacgagg ccccagtggc cgccgatgac ccgcgacacc aggccgtcgt
      421 ggcccaggtg gttgaggccc cggtccttgc cgtcgccctg gccggcggcg tagacaagcg
      481 tcaggttgcg cgggaagccg aggccctggc cttcgctgcc ctggttttcc aggtacagct
      541 cttccagggc gatggcgatg ttctccggaa agccgatgcc gacgaagccg ccggtggcga
      601 cggtgtcgcc gtcacggatc aggttgaccg cctcccgcgc ggacacgatc ttgccggtgt
      661 ccgaggcgcg cagggagttg gcgaggggat ggcgtgaaat gctcatggtg gtgtctcctc
      721 ccgggcaggt ccgcgagcgt tgttatacga ggccggtggc gtagtaggtg gcgatgatga
      781 cgaacaccgc agtggtcttg atcatcgtga tggcaaagat gtccttgtag gccaccttgt
      841 gggtcagacc ggtgacggcc agcagtgtga tgacggcgcc attgtgaggc agggtgtcca
      901 tgccgccgct ggccatggag gcgacccggt gcagcacttc cagcgggatg cccgcggcgt
      961 tggccgcctg gatgaaggtc tccgacatgg ccgccagggc aatgcccatg ccgccggacg
     1021 cggaaccggt gataccggcc agggtggtga cggtgatggc ttcattcacc agcgggttgg
     1081 ggatcacctt gagggcgtcg gccaccacca ggaagcccgg cagcgcggcg atgacggcac
     1141 cgaagccgta ttcggacgcg gtgttcatcg acgccagcag ggcgccgccc acggcagcct
     1201 tggtgccctc tgcgaacttg gtggagacgg tgcgccaggc gaataccatg accgtgagga
     1261 tgcccaccag cagggcgcct tccacggccc agatggcggc gatcttggag acgtcctgga
     1321 ccaccggctt ggcggtgccg atcacggccg gcacgaagga gtggctggtg ccgtagattt
     1381 gcgggatgag gtcggtgaac agcttgttgg tcacgcccac cagcaccagg ggcaggatgg
     1441 cgaggaccgg attggcaagc ttctcggtct cgtcgaaggg ttccggctcg ttcagcagtt
     1501 cggtgccgta gccttcgccc gcggacttgg cggtgcgacg acgccactca aggtagctga
     1561 ggccgacgat gaagatgaag accgcgccca cggtgcccag taccggggcg gcgtagatgt
     1621 tggtcttgaa gaaggtcgtg gggatcacgt tctggatctg cggcgtgccg ggcagggagt
     1681 ccatcgtgaa ggtgaaggcg cccagcgcga tggtgccggg gatcaggcgc ttggggatgc
     1741 cgccctgacg gaacatctcg gcggcaaacg gatacaccgc gaagaccacc acgaacaggg
     1801 acacgccgcc gtaggtgagc agtgagcaca ccagcacgat ggagagcatc gcccgctcgg
     1861 cccctaccac cttgatgacg gaggagacga tggacttgga gaagccggac agttcgatga
     1921 ccttgccgaa gatggcgccg agcaggaaga ccgggaagta gttcttcacg aagccgacca
     1981 tcttgtccat gaagaggccg gtgaacatcg gggcgaccag ggtcgggtcg gtgagcagca
     2041 cggcgccgag ggccgcgacc ggcgcaaaca ggatgacgct gtagccccgg taggctacga
     2101 acatcaggaa gcacaaggct gccaatacaa tcaggaatga catggtgaga tctcctccgt
     2161 cgttaaaatg caaaactgtc ggggtgtcac tgcagattcc atgcctgctt ttttggggtt
     2221 gatttgtaag cagtttttgc gatgttgcgg tgcgtcgtgt ccggtgcggc aggcggcact
     2281 gtccgcggag ccggacagct tcaaggcgcg gcgatgccca gcgcggcgat cttctcgtag
     2341 agggatgcgc gggagatgcc caggctgcgt gcggcctggg ccttgttgcc accggccgcg
     2401 gcgatggcgt cggcgatggc ctgccgctcc gtggcctgct ggatctggtc gagggtgcgt
     2461 gcgttttcgg cgacggggcg cgcgggcggt cggaccagcc cgggcagcac ctgttcgaag
     2521 tcggcctggt cgaggcagtc ggcgtccgac atcagcaggg cccgctccag cacgttggcc
     2581 agctcgcgca cgttgccggg ccagctatgg tgggccaggt agtccagggc ggcgcggctc
     2641 aggctgcggg gcggctcgtc gagctggcgg gcgaggctgt cggtgagggc ttcggcgatg
     2701 aagggcaggt cgtccaggcg gtcgcgcagg ggcggtacgt cgagggtgat gacgttgagg
     2761 cggtagaaca ggtcggcgcg gaagcggccg gcctcgatct ccttcggcag gtcgcggctg
     2821 gtggcggcga tgatgcgcac gtccacgctg atcaggcggt tggagccgac cggctcgatc
     2881 tccttttcct gcagggcgcg cagcagcttg gactggagcg ccggcgacat gtcgccgatc
     2941 tcgtcgagga agagggtgcc gccctgggcg agttcgaact tgccgctgcg ccccttgcgc
     3001 tcggcgcccg tgtaggcacc cggcgcaacg ccgaagaatt cggcctccag cagggtttcc
     3061 ggcacggcgg cgatgttgac cgccacgaag gggccgccgg cgcgggggct ggcggcgtgg
     3121 atggcgtggg cgaggagctc cttgccggtg ccggtctcgc ccaggatgag gaccggcgag
     3181 gctgcgcggg cggcgcgccg ggccttgttc ttgacggcca cgcagcgcgg gctggcgccg
     3241 atgaagctgg caaaggtgta tttgctgcgg cgggcctcgt cgagcttgcg ccgggtgtcg
     3301 gccagttcgg tgcgcaggct ctggaagcgg gagaccaccg gggcgagctg gcgggcgtcg
     3361 tcgaagatga ccatgcccac gccgccgatg acctcgccat gctcgtcgcg caccggcagg
     3421 cgcatgacga cgacggattc gtgccccagt tccatgatgt ccagcatgat gggtttgccg
     3481 gtgctcacca cctggcgcat caggctgttg gggatgacct cttccaccgg gcggccgatg
     3541 atgtcggcgg cgtcatggac catgagatgg cgtggatact tgtcgttcat ccacacgatg
     3601 cgggcgtccc ggtccacgat gaccacgcct tcggacagct cggcgaattc ggcgagcagg
     3661 gtgtcgatga cgcgctggcg gatgcgctcc gcgtccgcga ggaagtgttc gggcgacatg
     3721 gtgggcagcc ttgttcttgt gggggcgccg gtggggccgg aacggcctgt ctcctccggg
     3781 cgtgggtgca gcatagcgca cccgcgcggc accgcccgat acgggttcac cctgatgccg
     3841 ggcccgtcaa cgacacagcc cggcgcgggg ccgggctgca gggacgtcgc cgaggcgagg
     3901 gaatcaggcc ttgaccggga tcttgccgat cttgacctgc cattccttcg ggccggtgtt
     3961 gtgcaccgag gtgccggtgg agtccaccgc cacggtgacg ggcatgtcct gcacgtcaaa
     4021 ctcgtagatg gcttccatgc ccaggtcctc gaagcccacc accttggcgg acttgatggc
     4081 cttggccacc aggtaggcag cgccgccgac ggccatcagg taggcggagt tgttgtcctt
     4141 gatcgcctcg atggccacgg ggccgcgctc ggacttgccg atcatggaga tcaggccggt
     4201 ctgctcgagc atcatgcggg tgaacttgtc catgcgggtg gcggtggtgg ggccggccgg
     4261 gccgacgacc tcgtcacgca ccgggtcgac cggtccgacg tagtagatga cccggttggt
     4321 gaagtccacc ggcagcttct cgcccttggc cagcatgtcg gcgatgcgct tgtgggcggc
     4381 gtcgcggccg gtgagcatct tgccgttgag gaggatcttc tggccgacct tccaggaggc
     4441 gacttcttcc ttggtcaggg tgtccagatt gacgcgggtg gcggacttga cgtcaggcgt
     4501 ccaggtgacg gcgggccagt cttccagctt gggcgggtcc agcttggcgg ggcccgagcc
     4561 gtccaggtgg aagtggcagt ggcgggtggc ggcgcagttg ggcaccaggg cgatgggcag
     4621 gttggcggcg tggcaggggt agtccagcac cttcacgtcc agcacggtgg tgaggccgcc
     4681 caggccctgc gcaccgacgc ccagggcgtt gatcttttcc atcagctcca ggcgcagcgc
     4741 ttcggcgcgg ctgagtgggg cgccggaggc ggccttggcc ttgagttcgt ggatgtccac
     4801 cggcgccatg atggattcct tggccagcag catggccttc tcgggcgtgc cgccgatgcc
     4861 gatgccgatg atgcccggcg gacaccagcc ggcgcccatc tcggggatct tgtgcag
//
LOCUS       DAOQPA010000181         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10754_length_4894_cov_18.534615, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000181 DAOQPA010000000
VERSION     DAOQPA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10754_length_4894_cov_18.534615"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKX01_16255"
     CDS             complement(<1..386)
                     /locus_tag="PKX01_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ77738.1"
                     /translation="MASYKEFHKRSIEDRDGFWGEQAQLIHWNKQPSQIYDFSNPPFV
                     KWFVGGETNLCYNAVDRHAESHPDRKALVYISTETDEEKVYSFAELRTEVMRMAAIYQ
                     DLGVKKGDRVLIYMPMIAEACFAMLA"
     gene            complement(459..1643)
                     /locus_tag="PKX01_16260"
     CDS             complement(459..1643)
                     /locus_tag="PKX01_16260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ77739.1"
                     /translation="MNDDSDQALPRVLIIDDSRMVRASLIRHLRGRFEVREEADGEDG
                     WQTLMVDPTIQAVITDIGMPRLDGYGLLERIRGSRLSRISTLPVAVISGDDEPEVRVK
                     AKSAGATDFISKSISTVELLARLEALTQLARTQRDLEESRAALATSSPVDPVSGLATP
                     SYLHHHAAQELSLAARRHTDVSVMVIEIDGFDTLVSRYGAHVAQLIHRKLSRILATKL
                     RQEDTVAELATARFAVVSPNTDIDGVAAFAFRLRQAINHMVMTYREERIHIHLTVGIA
                     ANSPDGRTTVSHLIADALQRLSTGRTLGGDRVIGPHGEVTEASVAQKAAARPPVSIDA
                     LLQRVRAGNATDVRGQLPDAIRTLLPLLELIESEFRSGLPLASLARAADTGLVAGFHN
                     NG"
     gene            complement(1669..2679)
                     /locus_tag="PKX01_16265"
     CDS             complement(1669..2679)
                     /locus_tag="PKX01_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HMZ77740.1"
                     /translation="MNTPLPADFQAFVIHQDAQRKVDSRMERMTVDQLDAGEVLIRVA
                     YSSVNYKDALAATGAGKIIRRFPCVGGIDLSGTVVSSADSRFKAGDPVIATSFDIGVA
                     HHGGYAEYARVPAGWVVPLPAGLSLFDAMALGTAGFTAALGIVRMEDNGLCPDNGPVI
                     VTGATGGVGGLAIDMLAGLGYHVVALTGKAEEEAYLRELGAAEIRLRSTIDPAKTRPL
                     DAGLWAGAVDNVGGDILAWVLSTMKQAGTVASIGNAQSISLNTTVFPFILRGVSLLGV
                     DSGYMGFPTRTRVWDRLATDLRPRHLESMSRTVPFADLPGVFDDFIAGRVKGRTVIAV
                     SA"
     gene            2832..3731
                     /locus_tag="PKX01_16270"
     CDS             2832..3731
                     /locus_tag="PKX01_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ77741.1"
                     /translation="MALITRPFETLSAYEAFFAHEEMPVLRHTVKELDAMREQEDHVN
                     GRAVAALVLSDPMMTLRVLIHLEQNRRASQNHDITTVERAIMMMGISPFLRRFADLPT
                     IEAQLHDHPKALIGVLRVISRARRAAKYARDWAIVRHDLDVDEITVAALLHEVAEIVC
                     WCFAPTLTDEVYAMQRADHSLRSVAAQRRVFNVSAQDLQSCLVRQWHLPELLVSMMDT
                     HQINNPRVATVKLAGNLARHTAQGWDNAAIPDDLAAIEALLHVGRETLLHRLAVPHED
                     ADRLLNAGGQAAGALPPEQSPQA"
     gene            complement(3722..>4894)
                     /locus_tag="PKX01_16275"
     CDS             complement(3722..>4894)
                     /locus_tag="PKX01_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ77742.1"
                     /translation="QLDAEEKANARFDKLLAQEEVWIRKGVEARRTRNEGRVRRLEAL
                     RRERGERRDRLGNVRLAVDRGESSGQMVAELEDVGKRFGERWVARHFSTRILRGDRIG
                     LIGPNGAGKTTLLKLILGEIEPDEGRVRRGTRQTVAYFDQLRAQLDPEAPLTEVISPG
                     SDYVEIGGHRTHVIGYLGDFLFSPARARSPVKSLSGGERNRLLLARLFARPANVMVLD
                     EPTNDLDIETLDLLEDLLARYDGTVFLVSHDRAFLDNVVTQVIAAEGGGDWKEYAGGY
                     EDWLRTRPAAVPAVAPVVPATPKTAPPPRKEAAPDRPAKLSWKEQRELEGLPDRIAAL
                     ETQQADVQARLGDPTLYKDAPQEVPVLKAQLEALEKDIDAALSRWEALESRAAAQA"
BASE COUNT          788 a         1661 c         1596 g          849 t
ORIGIN      
        1 caggccagca tcgcgaagca ggcttcggcg atcatcggca tgtagatcag gacgcgatcg
       61 cccttcttga cgccgagatc ctgatagatc gccgccatgc gcatgacctc ggtgcgcagt
      121 tcggcaaagc tgtagacctt ttcctcgtcg gtttcggtgg agatataaac caacgccttg
      181 cggtccgggt gggactcggc gtggcgatca acggcgttgt agcagaggtt ggtctcaccg
      241 cccacgaacc acttgacgaa gggcgggttg gaaaagtcgt agatctggga cggctgcttg
      301 ttccagtgga tcagttgagc ctgttcgccc cagaaaccat cccgatcctc gatggagcgc
      361 ttgtgaaatt ccttgtaaga agccatgggt ttcctctccc tttgttcgta tcgtgaaagc
      421 actgcatgcc ggggcggtac gcggatgcgt ccgtcacctt atccgttgtt gtggaagcca
      481 gccaccagcc cggtgtctgc agcgcgtgcg agggaggcaa gcggcagccc ggagcggaat
      541 tcggattcta tcaattccag cagcggcaag agtgtccgga tcgcatcggg cagctgaccg
      601 cgcacgtccg tcgcgttgcc ggccctgacg cgctggagca atgcgtcgat gctgaccggc
      661 ggccgcgcgg ctgccttctg cgcgacggag gcttcggtga cctcaccgtg aggcccgata
      721 acccggtcgc cgccgagggt ccgaccggtc gaaagtcgct gcagcgcatc cgcaatgagg
      781 tgactgaccg tggtccgccc atccggcgag ttggcggcaa tcccaactgt cagatggata
      841 tggatacgct cttcccgata ggtcatcacc atgtggttga tggcctggcg caggcggaag
      901 gcaaaggctg ccacgccgtc gatgtccgtg ttcggcgaca ccacggcaaa tcgtgccgtg
      961 gccagctctg cgacggtgtc ctcctgacgc agcttggtgg ccaggatgcg cgacagcttg
     1021 cggtggatga gctgggccac gtgggcgcca taccggctca ccagcgtatc gaacccgtcg
     1081 atctcgatga ccatcacgct gacatcggtg tgccgtcgcg ccgccaggga cagttcctgg
     1141 gcagcgtgat ggtgcaggta ggagggcgtc gccagcccgg agacgggatc cacggggctc
     1201 gacgtggcca gcgcggcacg actttcttcc aggtcccgct gggtgcgggc cagttgggtg
     1261 agcgcttcga ggcgggcgag cagttcgacg gtggagatgc tcttggaaat gaagtcggtg
     1321 gcgccggcgc tcttggcctt cacgcgcact tcgggctcgt cgtcgcccga tatgaccgcc
     1381 accggcaagg tggagatgcg cgacaggcgc gagccgcgga tgcgctccag cagcccgtaa
     1441 ccgtccagac gcggcatgcc gatgtcggtg atgaccgcct ggatggtcgg atccaccatc
     1501 agggtctgcc agccgtcttc cccgtccgct tcctcgcgga cctcgaaacg cccccgcagg
     1561 tgccggatca gggaagcccg gaccatgcgg gagtcgtcga tgatcaggac cctgggcagg
     1621 gcctgatcgc tgtcgtcatt catgctgcgc tcgctgtgag gggtgtcctc atgcgctaac
     1681 ggcaatcacc gtgcggccct tgacccgccc cgcgatgaaa tcgtcgaaca cccccggcag
     1741 atctgcaaaa ggcaccgtgc gggacatgga ctcgagatga cgcgggcgca ggtcggtggc
     1801 cagccgatcc cagacccgcg tgcgcgtcgg gaagcccatg tagccggaat ccacccccag
     1861 cagactcacg ccgcgcagga tgaaggggaa gaccgtggtg ttcaggctga tgctctgggc
     1921 gttgccgatg ctcgccacgg tgccggcctg tttcatggtg gacagcaccc aggcgaggat
     1981 gtcgcccccc acgttgtcca ccgcgccggc ccacaggccc gcgtccagcg gccgcgtctt
     2041 ggccggatcg atggtcgaac gcaggcgaat ctcggccgca ccgagttccc tcagataagc
     2101 ctcttcctcg gccttgccgg tcagcgccac cacgtggtag ccgagaccgg ccagcatgtc
     2161 gatggccagc ccgcccacac cgccggtggc gcccgtgacg atcaccggac cgttgtccgg
     2221 gcacaggccg ttgtcctcca tgcgcacgat gcccagtgct gcggtgaagc cggccgtgcc
     2281 gagcgccatg gcatcgaaca gggagagccc cgccggcagc ggcaccaccc acccggcagg
     2341 cacccgcgcg tattcggcgt agcccccgtg atgggccacg ccgatgtcga agctggtggc
     2401 gatcaccgga tcgccggcct tgaagcgtga atccgcactg ctcaccaccg tgccggacag
     2461 gtcgatgcca cccacacagg gaaagcgccg gatgatcttg ccggcaccgg tggccgccag
     2521 cgcatccttg taattgacac tggaataggc cacgcggatc agcacttcgc cggcgtcgag
     2581 ctgatcaacg gtcatacgct ccatgcggct gtccaccttg cgctgcgcgt cctggtggat
     2641 cacgaaagcc tggaaatcgg cgggaagagg ggtgttcatg cggttctcct gatatagcgg
     2701 gcggctgccc tggttatggt tctgcgccga ttatatcgcc cgcccggcgg ctgtttccgg
     2761 cccgccgtgc gttgctcaat gtcgagcccc tgctgccgta aatgggaaca tcgcccccca
     2821 ggagaccgtc catggccctg atcacccgcc ctttcgaaac cctttccgct tacgaggcct
     2881 tcttcgccca cgaggagatg ccggtgctcc ggcacacggt gaaggagctc gatgccatgc
     2941 gcgagcagga ggatcatgtc aatggccgcg ccgtggcggc gctggtcctg agcgatccca
     3001 tgatgactct gcgggtattg atccacctcg agcagaaccg gcgggccagc cagaaccacg
     3061 acatcaccac cgtggagcgg gccatcatga tgatgggcat ctcgcccttc ctgcgtcgct
     3121 ttgcggacct gcccacgatc gaggcgcagc tgcacgatca tcccaaggcc ctgatcggtg
     3181 tcctgcgggt catcagccgg gcccgccgcg ccgccaagta cgcccgcgac tgggccatcg
     3241 tgcggcacga cctggatgtg gacgaaatca ccgtcgccgc cctcctccat gaggtcgcgg
     3301 agatcgtctg ctggtgcttc gccccgaccc tcaccgacga ggtctacgcc atgcagcggg
     3361 ccgaccacag cctgcgttca gtggctgcgc agcgccgcgt cttcaacgtc tcggcccagg
     3421 atctgcagtc ctgcctggtt cggcaatggc accttccgga actgctggtc tcgatgatgg
     3481 acacgcacca gatcaacaac ccccgggtcg ccacggtcaa gctcgcgggc aatctggccc
     3541 gtcacactgc ccagggctgg gacaacgcgg ccatcccgga cgatctagcc gcgatcgagg
     3601 cgctgctgca tgtgggccgg gaaaccctgc tgcatcgcct cgcggtgccc cacgaggacg
     3661 ccgaccgcct gctcaatgcc ggcggacagg ccgccggagc cctgccgccg gagcagtcgc
     3721 ctcaggcctg agccgcggcg cgggattcca gcgcctccca gcgcgacagg gcggcgtcga
     3781 tgtccttctc cagcgcttcc agctgcgcct tgagcacagg cacctcctga ggcgcatcct
     3841 tgtagagcgt cggatcaccc agccgggcct ggacgtccgc ctgttgagtc tcgagcgctg
     3901 caatgcggtc cggcaatcct tccagttccc gctgttcctt ccagctcaat ttcgcgggcc
     3961 ggtccggtgc tgcttcctta cgcggcgggg gtgccgtttt gggcgtcgcg ggcacaactg
     4021 gggcgacggc cggtacggcg gccggacgcg tacgcagcca gtcctcgtac ccgcctgcat
     4081 attccttcca gtcgcccccc ccttccgcgg cgatgacctg ggtcaccacg ttgtccagga
     4141 aggcccggtc gtggctcacc aggaagaccg tgccgtcgta gcgggccagc aggtcttcca
     4201 gcaggtccag cgtctcgatg tccaggtcgt tggtcggttc gtccagcacc atcacgttgg
     4261 ccgggcgggc gaacaggcgg gccagcagca ggcggttgcg ctcgccgccg gacagggatt
     4321 tgaccggtga tcgggcgcgg gccggcgaga acaggaagtc ccccagatag ccgatgacgt
     4381 gggtgcgatg cccgccgatc tccacgtagt ccgaacccgg actgatgacc tcggtcagcg
     4441 gcgcctccgg gtcgagctgg gcgcgcagct ggtcgaaata ggccacggtc tgacgggtgc
     4501 cgcggcggac gcggccttcg tcgggctcga tctcccccag gatcagcttg agcagcgtcg
     4561 tcttgccggc gccattggga ccgatcaggc cgatgcggtc gccgcgcagg atgcgcgtgg
     4621 agaaatggcg cgccacccag cgttcaccga agcgtttgcc cacgtcctcc agctcggcca
     4681 ccatctgccc gctgctttcg ccacgatcca ccgccagcct gacgttgccc aggcgatcac
     4741 ggcgctcacc gcgctcccgg cgcagggcct ccaggcgccg cacgcgcccc tcgttgcggg
     4801 tccggcgggc ctcgacgccc ttgcggatcc acacttcttc ctgggccagc agcttgtcga
     4861 agcgggcgtt ggccttctcc tcggcgtcga gctg
//
LOCUS       DAOQPA010000182         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10933_length_4839_cov_11.539716, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000182 DAOQPA010000000
VERSION     DAOQPA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10933_length_4839_cov_11.539716"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..398
                     /locus_tag="PKX01_16280"
     CDS             6..398
                     /locus_tag="PKX01_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ77743.1"
                     /translation="MCDAPYQAVAEQLGTTEAEVLARLRRLLDERVLTRFGPMFQVER
                     LGGRFVLAALAVPEARFDTVAAQVNALPEVAHNYRREHHLNMWFVLATETPDGIADAI
                     RRIEADTGLMVFAFPKRREFFVDMRLQA"
     gene            395..901
                     /locus_tag="PKX01_16285"
     CDS             395..901
                     /locus_tag="PKX01_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HMZ77744.1"
                     /translation="MNPPDDLDRRLVIATQAGLPLVPRPYDALAAQLGVSAEVVRARL
                     AAMLENGRIRRIGAVPNHYALGYTANGMSVWDVDDARIVELGEAVGRLPGVTHCYERP
                     RALPDWPYNLFAMVHGRDRATVLAQVETIARLLGAACRARDVLFSTAILKKTGLRIGR
                     GGGDGAAY"
     gene            complement(895..3570)
                     /locus_tag="PKX01_16290"
     CDS             complement(895..3570)
                     /locus_tag="PKX01_16290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ77745.1"
                     /translation="MSTAVRVTGRQLVIAGATFVTAIIAIVSVTAAMLLHQQEIDGWR
                     QQLASLSLALAEQTAQTMSATDLVLDSIVAEVDALHLHDAADLRALTRSETFHHFLKE
                     RVAGLSRADVATIVGANGEVINFTRSFPPPPINLSDRDYFKVHLADARVGTYISVPVR
                     NKGNGKWIFYVTRRLNDAEGRFAGLVLVGISVDQFSDFFQRIGASLGEGASVSLSRDD
                     FALLTRWPARENLIGERNLTGSNYEVIHTLGKSDDVISLDSPRMPEDFAPQRRMAAVR
                     KVEHFPLILNITVSHDLVLSNWYPAVWAIGAIAGGSILALLLGTFTGLRILARREDDA
                     RRLRESEERYARAVRGTSDGLWDWDLVAERDYLSPRLKDMLGFGGDPAITRMDDVLSR
                     VHPQDEPTVRKALRAQLDQHVPLDIELRVLTRGGSYRWMALRGEAERTPAGKPVRLSG
                     TLSDITARKLAEEEVLEHRNHLEDLVDARTAALSVAKEAAEAASRAKTAFLANMSHEL
                     RTPMNAIICLTHLLRRGANEPGQREKLAKITQSADHLLEIINNVLDLSKIEAERVSLE
                     RTHFRVSDVLHQVEGLLDEKARDKCLRLTLQADPAIAELDLLGDPLRLKQVLLNLTGN
                     AIKFTECGSVTVEAHRCGHTDEDVVIGFDVQDTGVGMSDGVLQRIFEPFEQADGSTTR
                     RYGGTGLGLAISRHLVELMGGQITVTSTPGAGSCFSFTVHLARAPAGDGGPDVSVLTP
                     DEAEQLLKQRYRGIRLLLAEDDLINQEVAMELLHHLTGLAVDLANDGVEAVEMARLGH
                     YDMILMDMQMPHLDGLAATRRIRALPGVGRTPIIAMTANAFEGDRRDCLEAGMDDFLA
                     KPFNPGSLLQVLLRWLPRPVVAPPPPARRRDARDQ"
     gene            3828..>4839
                     /gene="nirJ"
                     /locus_tag="PKX01_16295"
     CDS             3828..>4839
                     /gene="nirJ"
                     /locus_tag="PKX01_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HMZ77746.1"
                     /translation="MFRISSYIREIAVPTPLGPRRQPPGPVVIWNLIRRCNLTCKHCY
                     SISADTDFAGELSTDEVYRTMDDLKAFRVPVLILSGGEPLLRPDIFDIAARAKGMGFY
                     VGLSSNGTLIDAANIDRIAAADFDYVGISLDGIRDTHDRFRRLDGAFERSLAGLRRCR
                     DLGVKVGVRFTLTADNAHDLPAVLQLVEDEGIDRFYFSHLNYAGRGNKHRADDARHVL
                     TRQAMELLFETAWDYEQRGLVKDFTTGNNDADGPFFLHWVQRRFPERAAHIEAKLMQW
                     GGNASGVNVANIDNLGNVHPDTMWWHHTLGNVRERAFGAIWTDLSDPLLAGLKQSPRP
                     VSG"
BASE COUNT          726 a         1592 c         1749 g          772 t
ORIGIN      
        1 tccccctgtg tgacgcgccc taccaggcgg tggcggagca gctcggcacc accgaggccg
       61 aggtgctggc gcgcctgcgt cgtctgctgg acgagcgggt cctgacccgc ttcgggccca
      121 tgttccaggt ggaacgcctg ggcggacgct tcgtgctggc agcgctggcg gtgccggaag
      181 cgcgcttcga caccgtggcg gcccaggtca acgctttgcc cgaggtggcc cacaactacc
      241 ggcgcgagca ccacctcaac atgtggttcg tgctcgccac cgagacgccg gacggcatcg
      301 ccgacgcgat ccggcgcatc gaggccgaca ccggcctcat ggtattcgcc tttcccaagc
      361 ggcgggagtt ctttgtggac atgaggttgc aggcgtgaac ccgccggatg atctggaccg
      421 ccgcctggtg atcgcgaccc aggccggcct gcccctggtg ccgcggccct acgacgccct
      481 ggcggcacag ctgggggtga gcgccgaggt cgtccgggcg cgcctggcgg cgatgctcga
      541 gaacggccgc atccgccgca tcggtgcggt gcccaaccac tacgctctgg gctacacggc
      601 caacggcatg tccgtgtggg acgtggacga cgcccgcatc gtcgagctgg gcgaagcggt
      661 gggccgtctg cccggggtca cccactgcta cgagcgcccg cgcgccctgc cggactggcc
      721 ctacaacctg ttcgcgatgg tgcatggccg cgaccgggcc acggtgctgg cacaggtgga
      781 gaccatcgca cgcttgctgg gggcggcctg ccgggcccgt gacgtgctgt tctcgacggc
      841 gatcctgaag aagaccgggc tgcgcatcgg ccgcggcggc ggggacggcg cggcctactg
      901 atcgcgggcg tcgcggcgac gcgcgggggg tggcggcgcg accaccggcc ggggcagcca
      961 gcgcagcaga acctgaagca ggctgcccgg attgaacggc ttggcgagga agtcgtccat
     1021 gccggcttcc aggcagtcgc ggcgatcccc ttcgaaggcg ttggcggtca tggcgatgat
     1081 gggtgtgcga ccgaccccgg gcagggcgcg gatgcgacgg gtggccgcca gaccatccag
     1141 gtggggcatc tgcatgtcca tcaggatcat gtcgtagtgg ccgaggcggg ccatctccac
     1201 cgcctcgacg ccatcgttgg ccagatccac ggcgaggccg gtgaggtgat ggagcagctc
     1261 catggccact tcctggttga tcaggtcgtc ctcggccagc agcaggcgga tgccccggta
     1321 gcgctgcttg aggagctgct cggcttcgtc gggggtcagg acggacacgt cgggcccgcc
     1381 gtcgccggcc ggcgcccgcg ccagatggac cgtgaagctg aagcagctgc ccgcgcccgg
     1441 cgtgctggtc acggtgatct gtccgcccat gagctccacc agatggcggg agatcgccag
     1501 ccccaggccg gtgccgccgt agcgccgggt ggtggagccg tcggcctgct cgaagggctc
     1561 gaagatgcgc tggaggacgc cgtccgacat gcccacgccg gtgtcctgca cgtcgaagcc
     1621 gatcaccacg tcctcgtcgg tgtggccgca gcggtgcgcc tcgacggtga cgctgccgca
     1681 ttcggtgaac ttgatggcgt tgccggtgag gttgaggagc acctgcttca ggcgcagggg
     1741 atcgcccagc aggtccagtt cggcgatggc cgggtcggcc tgcagggtga ggcgcaggca
     1801 cttgtcacgg gccttttcgt cgagcaggcc ttccacctgg tgcagcacgt cactgacgcg
     1861 gaagtgggtg cgttccaggc tcacgcgctc ggcctcgatc ttggacagat cgaggacgtt
     1921 gttgatgatc tccagcaggt gatcggcgga ctgggtgatc ttggcgagct tttcccgctg
     1981 gccgggctca tttgcgccgc gccgcagcag gtgggtgagg cagataatgg cgttcatcgg
     2041 cgtacgcagc tcgtggctca tgttggcgag gaacgcggtc ttggcgcggc tggcggcctc
     2101 ggcggcctcc ttggcgacgg acagcgcggc ggtgcgggca tccaccagat cctcgaggtg
     2161 attgcggtgc tcgaggactt cctcttcggc cagcttgcgg gcggtgatgt cgctgagggt
     2221 gccggacagg cgcacgggct tgccggcggg tgtgcgctcg gcctcgccgc gcagggccat
     2281 ccagcggtag ctgccgccgc gggtgagcac gcgcagttcg atgtcgaggg gcacgtgctg
     2341 gtccagctgg gcccgcaagg ccttgcgcac ggtgggttcg tcctggggat ggacgcggct
     2401 caggacgtcg tccatgcggg tgatggcggg atcgccgccg aagcccagca tgtccttgag
     2461 gcgcggtgac aggtagtccc gctccgccac caggtcccag tcccacaggc cgtcgctggt
     2521 gccgcgcacg gcgcgggcgt agcgctcctc gctctcgcgc aggcgtcggg catcgtcctc
     2581 gcggcgggcg aggatgcgca gcccggtgaa cgtgcccagc agcagggcga ggatgctgcc
     2641 accggcgatg gcaccgatgg cccatacggc cgggtaccag ttgctcagga ccaggtcgtg
     2701 gctgacggtg atgttcaata tcagcgggaa gtgctccacc ttgcgcacgg cggccatgcg
     2761 tcgctggggg gcgaaatcct cgggcatgcg gggcgagtcg agggagatga cgtcgtcgga
     2821 tttgcccaga gtgtggatca cctcatagtt gctgcccgtg aggttgcgct caccgatcag
     2881 gttctcccgg gcgggccagc gggtgagcag ggcgaagtca tcgcgggaca gggacacgga
     2941 ggccccctcg ccgagggagg cgccgatgcg ctggaagaag tcggagaact ggtccaccga
     3001 aatgcccacc aggaccagcc cggcaaagcg cccctcggcg tcgttgaggc ggcgggtgac
     3061 gtagaagatc cacttgccgt tgcccttgtt gcgcaccggc acgctgatgt aggtgcccac
     3121 gcgggcatcg gccagatgaa ccttgaagta gtcccggtcg gacaggttga tgggcggtgg
     3181 cggaaagctg cgggtgaagt tgatgacttc cccgttggcg ccgacgatgg tcgccacgtc
     3241 ggcccgggac agcccggcca cgcgctcctt gaggaagtgg tggaaggtct cggagcgggt
     3301 cagggcgcgc aggtcggcgg cgtcgtgcag gtgcagggca tcgacctcgg cgacgatgct
     3361 gtccagcacc agatcggtgg cggacatggt ctgggcggtc tgctcggcca gggccaggct
     3421 gaggctggcg agttgctggc gccagccgtc gatctcctgc tggtgcagga gcatcgctgc
     3481 cgtcaccgag acgatggcga tgatcgcggt gacgaaggtg gcaccggcga tgacgagctg
     3541 ccggccggtg acccggacgg cggtggacat cccggtcacg gccggctccg gatttgggcg
     3601 ctgcagaaca cggcgagggg agtcggctgc atggcgggat cgagacgggc ggtgggattg
     3661 acacgcgacg acgccgtcgg tgcaccgggg ctccgggatt gcccggcagc cttgcaatat
     3721 aacggcccat tccggaccgg acttgagacc gcgggattga tgggcgtcaa tgcccgcagg
     3781 cgggcagggc gccgacaatc cggacaccct cttcaggaaa tccggccatg ttccgcatct
     3841 ccagctacat ccgcgaaatt gccgtgccca cgcccctcgg accgcgccgc cagccgcccg
     3901 gcccggtggt gatctggaac ctgatccggc gctgcaacct cacctgcaag cattgctact
     3961 ccatttcggc tgacacggat ttcgccggcg aactgagcac cgacgaagtc tatcgcacga
     4021 tggacgacct gaaggccttt cgtgtgccgg tgctgatcct gtccggcggc gagcccctgc
     4081 tgcgaccgga catcttcgac atcgcggccc gcgccaaggg catgggtttc tacgtgggcc
     4141 tgtcgtccaa cggcacgctg atcgacgccg ccaacatcga ccgcatcgcc gccgcggact
     4201 tcgactacgt cggcatcagc ctggacggca tccgcgacac ccacgaccgc tttcgccgcc
     4261 tggacggcgc cttcgagcgc tccctggcgg gcctgcgccg gtgccgcgac ctgggcgtga
     4321 aggtgggcgt gcgcttcacc ctcaccgccg acaacgccca cgacctgccc gccgtgctgc
     4381 agctggtgga ggacgaaggc atcgaccgct tctacttttc gcatctcaac tacgccggcc
     4441 gcggcaacaa gcaccgcgcc gacgatgccc gccacgtcct cacccgtcag gcgatggagc
     4501 tgctcttcga gaccgcctgg gactacgagc agcgcggtct cgtcaaggac ttcacgaccg
     4561 gcaacaacga tgccgacggg cccttcttcc tgcactgggt gcagcgccgc tttcccgagc
     4621 gggccgccca tatcgaggcc aagctgatgc agtggggcgg caacgccagc ggtgtgaacg
     4681 tggccaacat cgacaacctg ggcaacgtcc atccggacac catgtggtgg caccacaccc
     4741 tgggcaatgt gcgggaacgc gccttcgggg cgatctggac cgatctctcc gaccccctgc
     4801 tggccggcct caagcagtcg ccgcggcccg tcagcgggc
//
LOCUS       DAOQPA010000183         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_10994_length_4821_cov_14.826899, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000183 DAOQPA010000000
VERSION     DAOQPA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10994_length_4821_cov_14.826899"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKX01_16300"
     CDS             complement(<1..1151)
                     /locus_tag="PKX01_16300"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMZ77747.1"
                     /translation="MFEQAFKNIDDALWKESGCTTELDYTEQTSWLLFLKYLDGLEQD
                     KAAEALLEGKRYTYILDVPYRWESWAAPKDADGKLDHNTAITGDDLVDFVDRKLFPYL
                     HGFKQRATGPNTIEYKIGEVFGEIKNKIRSGYTLRDIIDHVDALRFRSQVEKHELSAL
                     YEEKIKRMGNAGRNGGEYYTPRPLIRAMIRVVDPQIGERIYDGACGSAGFLCEAHDYL
                     VARPNLSTTDLQILQTRSFYGKEKKSLAYVIAIMNMILHGIEAPNIVHTNTLAENLAD
                     IQDKDRMDVILANPPFGGKERAEVQQNFPIRTGETAFLFLQHFIKMLKAGGRAAIVIK
                     NTFLSNTDNASVSLRKLLLQSCNLHTILDCPGGTFQGAGVKTVVLCFEKG"
     gene            complement(1428..2114)
                     /locus_tag="PKX01_16305"
     CDS             complement(1428..2114)
                     /locus_tag="PKX01_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77748.1"
                     /translation="MPKIYYLPTDDAGKAELLEHLAARLPFYAEQLNVSTGELSSLNA
                     DASAYRYTMTMNNQAQHSAKNWVAFKNQLRDGGANGNGSFPPISLAGGAAPEAVAPGI
                     IPRLTTFVTRLKTAPNYTEAIGQDLGLIGTVRVVDPSVWQPAIATHTEAGRPVLAWSK
                     GGADALEIWVERTPQGGFVALDISTDTRYQDDEPTPATPAVWRYKAIFRLKDEPVGNW
                     SDVASVVVGG"
     gene            complement(3166..4710)
                     /gene="ahpF"
                     /locus_tag="PKX01_16310"
     CDS             complement(3166..4710)
                     /gene="ahpF"
                     /locus_tag="PKX01_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HMZ77749.1"
                     /translation="MLDATLKTQLQAYLTKLTRPVDIRAALDDGETARQMAELLQEVA
                     ALSPLLNYVEAADAGVRTPSFQISRPGENTGIRFAAIPLGHEFTSLVLALLQVGGHPP
                     RVSDDTLAQIRALEGEFRFVTYISLSCQNCPEVVQSLNLMALINPRISHETVDGALFQ
                     DEVERLKIMGVPAIYLNGELFGQGRMELEEILAKVDSGAAARAAASIDARAPYDVLVV
                     GGGPAGASAAIYAARKGIRTGVVTERFGGQVLDTLAIENFISVKETEGPRLAAALEEH
                     VRQYEVDIVNLQRAEALVPGELIEVKLASGASLKARTVILSTGARWRDMNVPGETEYR
                     TKGVTYCPHCDGPLFKGKAVAVIGGGNSGVEAAIDLAGVVAHVTLLEFDSKLRADAVL
                     VRKLESLPNVRILVNAQTTQVNGDGEKVTGLTYKDRVSGEVHDVALAGIFVQIGLVPN
                     TAWLKGTIELSTRGEIIVDDHGRTSVPGVFAAGDVTTVPYKQIIIAMGDGAKASLSAF
                     DHLIRN"
BASE COUNT          716 a         1415 c         1824 g          866 t
ORIGIN      
        1 cccttctcga agcacagcac cacggtcttg acgccggcgc cctggaaggt gccgccgggg
       61 cagtcgagga tggtgtggag gttacagctt tgcagcagca gcttgcgcag gctcaccgag
      121 gcgttgtcgg tgttcgacag gaaggtgttc ttgatgacga tggcggcccg gccgccagcc
      181 ttgagcatct tgatgaagtg ctggaggaac aggaaggcgg tttcgccggt gcggatcggg
      241 aagttctgct gcacctcggc gcgttccttg ccgccgaagg gcgggttggc gaggatcacg
      301 tccatgcggt ccttgtcctg gatgtcggcg aggttctcgg ccagggtgtt ggtgtggacg
      361 atgttgggcg cctcgatgcc gtgcaggatc atgttcatga tcgcaatcac gtaggcgagg
      421 ctcttcttct ccttgccgta gaagctgcgt gtctgcagga tctggagatc ggtggtgctt
      481 agattgggcc gggcgacgag gtagtcgtgg gcttcgcaca agaagccggc cgagccgcag
      541 gcgccgtcgt agatgcgctc gccgatctgc gggtcgacaa cgcggatcat ggcgcggatc
      601 agcgggcggg gcgtgtagta ctcgccgccg ttgcgcccgg cgttgcccat gcgcttgatc
      661 ttctcttcgt agagggccga cagctcgtgc ttttcgacct gggagcggaa acgcagggcg
      721 tccacgtggt cgatgatgtc gcgcagggtg tagccgctgc ggatcttgtt cttgatctcg
      781 ccaaagacct cgccgatctt gtattcgatg gtgttggggc cggtggcgcg ctgcttgaac
      841 ccatgcaggt agggaaagag cttgcgatcg acgaagtcca ccagatcgtc gccggtgatg
      901 gcggtgttgt ggtcgagctt gccgtcggca tccttggggg cggcccagct ttcccagcgg
      961 taggggacgt cgaggatgta ggtgtagcgc ttgccttcca gcagggcctc ggcggctttg
     1021 tcctgctcca ggccatcgag atacttgagg aagagcagcc atgaggtctg ctcggtgtag
     1081 tcgagttcgg tggtgcagcc gctctctttc cagagggcgt cgtcgatatt cttgaaggct
     1141 tgttcaaaca tgtaaggcgg aatgtgtagc gatgcggccc gtggccaatg cgccagttta
     1201 ctcactcctg cccgggcccg atatggctca gccttcaccc acccagcatg acatgggggc
     1261 agatggcgcc ggggcaatgc tgacgtttcc ggcatcgatc tgccatccga tgccggaaac
     1321 gtgtcgttta aagcgcagcg gggcctttcc gacgcgccat accgatcttt ggccatgaag
     1381 ggctcaccca gcgcagggtg cgcgccgttg cgagcccctt ttgggcctta cccgcccacc
     1441 accacactcg ccacgtcgct ccagttgccc acgggctcgt cctttagccg gaagatggcc
     1501 ttgtagcgcc acacggcggg ggtggcaggg gtgggctcgt cgtcctggta gcgggtgtcg
     1561 gtgctgatgt cgagggcgac gaagccgccc tggggcgtgc gttcgaccca gatctccagg
     1621 gcgtcggcgc cgcccttgga ccaggcgagg acggggcggc cggcttcggt gtgggtggcg
     1681 atggcgggct gccagacgct ggggtccacg acgcggacgg tgccgatgag gcccaggtcc
     1741 tgcccgatgg cttcggtgta gttgggggcg gtcttgaggc gcgtgacgaa ggtggtcagg
     1801 cgcggaatga tgccgggtgc cacggcctcg ggcgcggcgc cgcccgccag gctgatgggc
     1861 ggaaagctgc cgttgccgtt ggcgccgccg tcccgcagct ggttcttgaa ggcgacccag
     1921 ttcttggcgc tgtgctgggc ctggttgttc atggtcatcg tataacggta ggcggacgcg
     1981 tcggcgttga ggctggacag ctcgccggtc gagacgttga gttgttcggc atagaagggg
     2041 agccgggcgg ccagatgttc cagcaactcg gccttgccgg cgtcgtcggt gggcaggtag
     2101 tagatcttgg gcatgacgtg tctcctcgtg gaggggtggg taaaacattc gccctgcgcg
     2161 gcccggacgc cgccgcgcgg ggggtggagg gaagtgtccg agccgtctgg caggccttgc
     2221 cagacgccgg gcgaggcgtg ccagacgtgt agcaaacctc gccagacgtc tggcgagctt
     2281 cgccagcgcg cgggcgagcc gtgccagaca ccccaccctg gcgtgccaga cgccggtcca
     2341 gcctcgatag gcgtctggca gggctgctac acgccgggcg agcttcgcca gacgtgtagc
     2401 acgcctcgcc aggcgtctgg caggacaggg ggcaccgtgt ggcacgcctc gccagacgcc
     2461 gggcgagctt cgataggcct ctggcaaggc ctgccacacg gccttgatcg gcggcgtgag
     2521 gtccggcaga ccgagcaggc ggcggggcac agttcatgag acgccggggc aggtataaat
     2581 atacatattc gatatattta tggcatttat gcgtgcatgt caaaggcgtt tcagatgttt
     2641 tctttgtcat ttttctaata gctgccatta cgtttactgc acgggcacgc gggcatcccc
     2701 tgaacgacgc ctgccccaac gggccgggca ggacgcggcc ctggcgcagc actgcactgg
     2761 gctaggcgaa tgcgccgcag accggcgacc ggcacgcggt gggtgtgcgc ggcagcccgc
     2821 gcgatggaca tggcggtgga tgggggccgg gcgcggacgg gcatggcacg gcgggcggca
     2881 taccgtggac cgatggcgaa ggacggcgcc ggaagggagg cgatccggac gggccagggg
     2941 agcacgcggc tcaggcaggc gcgggttggt gcttcagggg ggcgggcgga acgcagggaa
     3001 gggcttgcga cgatggccgc cgtccggtgg gggtgcggcg gccatcgcgc ggggggcgcg
     3061 cggggcgctg gcgggtgcac cctcagccgg tcatgctaag ggcgctccgc ctggacccgg
     3121 cccgggtacg gatgtgcccg aagccggggc ggaggtgggc agcggtcagt tgcggatcag
     3181 gtgatcgaag gccgacaggg aggccttggc gccgtcgccc atggcgatga tgatctgctt
     3241 gtagggcacg gtggtcacgt cgccggcggc gaagacgccg ggcacggagg tgcggccgtg
     3301 gtcgtccacg atgatctcgc cgcgggtcga cagttcgatg gtgcccttga gccaggcggt
     3361 gttgggcacc aggccgatct gcacgaagat gccggcgagg gcgacgtcat gcacctcgcc
     3421 gctgacccgg tccttgtagg tgaggccggt gaccttttcg ccgtcgccat tcacctgggt
     3481 ggtctgggcg tttaccagga tgcgcacatt gggcaggctt tcgagcttgc ggaccagcac
     3541 cgcgtcggcg cgcagtttgc tgtcgaactc cagcagggtg acgtgggcga ccacgccggc
     3601 caggtcgatg gcggcctcga cgccggagtt gccgccgccg atcaccgcca cggccttgcc
     3661 cttgaagagg gggccgtcgc agtgggggca gtaggtgacg cccttggtgc ggtactcggt
     3721 ctcgccgggc acgttcatgt cgcgccagcg ggcaccggtg gacaggatca ccgtcctggc
     3781 cttgaggctg gcgccgctgg ccagcttcac ctcgatcagc tcgcccggca ccagggcttc
     3841 ggcgcgctgc aggttgacga tgtccacctc gtactggcgc acgtgctctt ccagcgcggc
     3901 ggcgaggcgc gggccttcgg tctccttgac ggagatgaag ttctcgatgg cgagggtgtc
     3961 cagcacctgg ccgccgaagc gctcggtgac cacgccggtg cggatgccct tgcgcgcggc
     4021 gtagatggcg gccgaggcgc cggccgggcc gccgcccacc accagcacgt cgtagggcgc
     4081 gcgggcgtcg atgctggccg cggcgcgggc ggcggcgccg gaatccacct tggcgaggat
     4141 ctcttccagc tccatgcggc cctggccgaa gagctcgccg ttgaggtaga tggcgggcac
     4201 gcccatgatc ttgaggcgct ccacttcgtc ctggaacagg gcgccgtcca cggtctcgtg
     4261 ggagatgcgc ggattgatga gggccatcag gttgagggac tggaccacct cggggcagtt
     4321 ctggcaggac agggagatgt aggtgacgaa gcggaactcg ccttcgaggg cgcggatctg
     4381 ggcgagggtg tcgtcgctga cgcggggcgg atggccgccc acctggagca gggccagcac
     4441 cagggaggtg aattcgtggc ccaggggaat ggcggcaaag cggatgccgg tgttctcgcc
     4501 ggggcggctg atctggaagc tgggggtgcg cacgccggca tcggcggctt ccacgtaatt
     4561 cagcaggggc gacagggccg ccacttcctg caggagctcg gccatctgcc gcgcggtctc
     4621 gccgtcgtcc agggcggcgc ggatgtccac cgggcgggtg agcttggtga gataggcctg
     4681 gagttgggtc ttgagggtgg cgtccagcat gatgggctcc ttgaaaaagt cagaatccgg
     4741 ataaagcaac gcccgggcgc cggcaggctg cccgggcgtt gcggtacgac aggtgtccgg
     4801 gcaggcggct tagatcttgc c
//
LOCUS       DAOQPA010000184         4786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11128_length_4786_cov_18.557599, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000184 DAOQPA010000000
VERSION     DAOQPA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11128_length_4786_cov_18.557599"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKX01_16315"
     CDS             <1..380
                     /locus_tag="PKX01_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ77750.1"
                     /translation="AIHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFAQADVAL
                     VIGANDVVNPSARTDKSSPIYGMPILNADLAQNVIVVKRGKGTGYSGIENALFYKDNC
                     RLLYGSAQQAVGEVIQHVKAMSV"
     gene            complement(484..3360)
                     /locus_tag="PKX01_16320"
     CDS             complement(484..3360)
                     /locus_tag="PKX01_16320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMZ77751.1"
                     /translation="MELAKSFEPADIERRWYQHWESSGYFAAGLDRSKPADQSFCILL
                     PPPNVTGTLHMGHGFNQTIMDALTRYHRMRGWNTLWQPGTDHAGIATQIVVERQLDAQ
                     GVSRHDLGREKFLEKVWEWKDYSGGTITRQMRRLGTSPDWSRERFTMDAGLNKIVTET
                     FVRLYREGLIYRGKRLVNWDPKLHTAVSDLEVVQEEEDGFLWHLNYPLADGSGHLTVA
                     TTRPETMLGDTAVMVHPDDERYAHLIGKTVRLPLVERDIPIIADAYVDREFGTGVVKV
                     TPAHDFNDYAVGLRHGLPMIIVLTLEGAIPATGTVVEGGVATGSAPLPADLVGLDRFD
                     ARKAVVERFEALGLLGEVKPHKLQVPRGDRTNAVIEPMLTDQWFVAMSKAGADGRSIT
                     EKALEVVASGEIKFVPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYGEDGQTFVA
                     HDEAEARAHATAAGYSGPLSRDEDVLDTWYSSALWPFSTLDWTPDYPARSNDALDLYL
                     PSTVLVTGFDIIFFWVARMVMMTTHITGKIPFRDVYVHGLIRDGEGQKMSKSKGNVLD
                     PIDLIDGIGVDDLVAKRTTGLMNPRQAASIEKKTRKEFPNGIPAFGTDALRFTFASLA
                     SPGRDIKFDMNRCEGYRNFCNKLWNATRFVLMNVQGHDLGLEHQQNGPSCGGNGPLAF
                     SFADRWIVSRLQRVEQDVALHFAEYRFDLVSKAIYEFVWDEYCDWYLELAKVQINTGD
                     EASQRGTRRTLIRVLETVLRLAHPLIPFVTEELWQTVAPLAGRKDTESICLARYPEAD
                     LGRCDETSEGDVAELKALIHACRNLRGEMNISPAQRMPLVACGNATRLTRFAPYIAGL
                     AKLSEVQVVDEIGSDELAPVAVVGEQRLMLKVEIDIAAERERLGKEIARLEGEIAKAK
                     AKLANESFVARAPAAVVAQEQERLAAFGATLDTLRPQLAKLSAA"
     gene            complement(3434..3871)
                     /locus_tag="PKX01_16325"
     CDS             complement(3434..3871)
                     /locus_tag="PKX01_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77752.1"
                     /translation="MSDSHRPVLEVPLEDEDGEHLLAKADALMRRHGHFAAPAEPDDL
                     PVLTDVVDYPGEEAPPDDSPPDITPVALPNARDDQTARIVDLEAAITRSIDAWVSTEL
                     PQIMSREFDAFAERLREAAREHMRTTLLPTLTAQIEKTLDTEE"
     gene            complement(3868..4299)
                     /locus_tag="PKX01_16330"
     CDS             complement(3868..4299)
                     /locus_tag="PKX01_16330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HMZ77753.1"
                     /translation="MPEIRFYHNAPDRLRVACSVTAKAWGQGRRISVLATDGAQARHF
                     DQLLWSFQPLAFVPHVGAESPLAAETPVVIGTHLDALHHEDVLINLGGELPPGFERFG
                     LVVEIVGQDEFERHAARQRFRAYKQAGFPLHAHDLAQRSGA"
     gene            complement(4314..>4786)
                     /locus_tag="PKX01_16335"
     CDS             complement(4314..>4786)
                     /locus_tag="PKX01_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HMZ77754.1"
                     /translation="QTIEVLNTDAEGRLILCDALTYAERYEPAAVVDIATLTGACIIA
                     LGNLTSGLLANDDALAAELLAAGQSAGDKAWQLPLWDEYQDMLKSNFADIPNIGSKGA
                     GTITAACFLARFTKAYTWAHLDIAGTAWKSGADKGATGRPVPLLVEFLARRAGA"
BASE COUNT          698 a         1442 c         1798 g          848 t
ORIGIN      
        1 tcgccatcca cccggtggcg ggccgcatgc cgggccacat gaacgtgctg ctggcggaag
       61 ccggggtgcc ctacgacaag atcttcgacc tggaagagat caacgccgac ttcgcccagg
      121 ccgacgtggc cctggtgatc ggcgccaacg acgtggtgaa cccctccgcc cgcacggaca
      181 agtccagccc gatctacggc atgcccatcc tcaacgcgga tctggcgcag aacgtgatcg
      241 tcgtcaagcg cggcaagggc acgggctact cgggcatcga gaacgccctg ttctacaagg
      301 acaactgtcg tctcctctac ggttcggccc agcaggccgt cggcgaggtg atccagcacg
      361 tgaaggccat gtcggtctga tcgcttgtcc caggctcacc gccaacgaaa acgcccgcgc
      421 aagcgggcgt tttccattgg agtgccggat ggggcagccg gcgggggcgc cgaccgtgcc
      481 cgatcaggcg gcgctgagct tggcgagctg gggccgcagt gtgtccagcg tggcgccgaa
      541 ggccgcgagg cgctcctgct cctgggccac taccgccgcc ggtgcgcggg ccacgaagct
      601 ttcgttggcg agcttggcct tggccttggc gatctcgcct tcgagacggg cgatctcctt
      661 gcccaggcgc tcccgctctg cggcgatgtc gatttccacc ttgagcatca gacgctgctc
      721 gcccaccacg gccaccggag cgagctcgtc gctgccgatc tcgtccacca cctgcacttc
      781 ggagagcttg gccaggccgg cgatgtaggg cgcgaagcgc gtcaggcggg tggcgttgcc
      841 gcaggccacc aggggcatgc gctgggccgg ggagatgttc atctcgccgc gcaggttgcg
      901 gcaggcatgg atcagggcct tgagctcggc cacgtcgcct tcactggtct cgtcgcagcg
      961 gcccaggtca gcttcggggt agcgggcgag gcagatgctc tcggtgtcct tgcggccggc
     1021 caggggcgcg acggtctgcc acagttcttc ggtgacaaag gggatcagcg ggtgggcgag
     1081 gcgcaggacg gtttcgagaa cgcggatcag ggtgcggcgg gtgccacgct ggctggcctc
     1141 gtcgccggtg ttgatctgca ccttggcgag ctccagatac cagtcgcagt actcgtccca
     1201 gacgaactcg tagatcgcct tggagaccag gtcgaagcgg tattcggcaa agtgcagggc
     1261 cacgtcctgc tcgacgcgct gcaggcggct gacgatccag cggtcggcga aggagaaggc
     1321 cagcgggccg ttaccgccgc agctggggcc gttctgctgg tgttccaggc ccaggtcgtg
     1381 gccctggacg ttcatcagca cgaagcgggt ggcgttccac agcttgttgc agaagttgcg
     1441 gtagccctcg cagcggttca tgtcgaactt gatgtcgcgg ccggggctgg ccaggctggc
     1501 gaaggtgaag cgcagggcgt cggtgccgaa ggcggggatg ccgttgggga attccttgcg
     1561 ggtcttcttt tcgatgctgg cggcctgtct ggggttcatc aggccggtgg tgcgcttggc
     1621 caccaggtcg tccacgccga tgccgtcgat gaggtcgatg ggatcgagca cgttgccctt
     1681 ggacttggac attttctggc cttcgccatc gcggatcagg ccatgcacgt acacgtcgcg
     1741 gaaggggatc ttgccggtga tgtgggtggt catcatgacc atgcgcgcca cccagaagaa
     1801 gatgatgtcg aagccggtga cgagcaccgt agacggcagg tagagatcca gcgcgtcgtt
     1861 cgaccgggcc gggtagtcgg gcgtccagtc cagcgtcgag aagggccaca gggcggagga
     1921 gtaccaggtg tcgaggacgt cctcgtcacg gctgaggggg ccgctgtagc cggccgccgt
     1981 ggcgtgggcg cgggcttcgg cttcgtcgtg ggcgacgaag gtctggccgt cctcgccgta
     2041 ccaggcagga atctggtggc cccaccagag ctggcgggag atgcaccagt cctggatgtt
     2101 gttgagccac tggttgtagg tgttgaccca gttttcgggg acgaacttga tctcgccgga
     2161 ggcgacgacc tccagggcct tttcggtgat ggacctgccg tcggcgccgg ctttgctcat
     2221 ggcgacgaac cattggtcgg tgagcatggg ctcgatgacc gcgttggtgc ggtcgccgcg
     2281 gggcacctgg agcttgtggg gcttgacctc gccgagcagg ccgagggctt cgaagcgctc
     2341 gaccaccgcc ttgcgggcgt cgaagcggtc gagcccgacc aggtccgccg gcaggggcgc
     2401 gctgcccgtg gcgacaccgc cttcgaccac cgtgccggtg gcggggatgg cgccttcgag
     2461 agtcagcacg atgatcatcg gcagaccgtg gcgcaggccg acggcgtagt cgttgaagtc
     2521 gtgggcgggg gtcaccttga ccacgccggt gccgaactcg cgatccacgt aggcgtcggc
     2581 gatgatcgga atgtcccgct ccaccagcgg caggcggacg gtcttgccga tcaggtgggc
     2641 gtagcgttcg tcatccggat gcaccatcac ggcggtgtcg ccgagcatgg tctcaggccg
     2701 cgtggtggcc accgtgagat gaccgctgcc gtcggccagc gggtagttga ggtgccacag
     2761 gaagccgtct tcctcttcct gcaccacctc caggtcggag accgcggtgt gcagcttggg
     2821 atcccaattg accagacgct tgccccggta gatcaggcct tcccggtaga ggcggacgaa
     2881 ggtctcggtg acgatcttgt tgaggccggc gtccatggtg aagcgctcgc ggctccagtc
     2941 ggggctggtg cccaggcggc gcatctgacg ggtgatggtg ccgcccgagt agtccttcca
     3001 ttcccagacc ttctccagga acttctcgcg gcccaggtcg tggcggctga cgccctgggc
     3061 gtcgagttga cgttccacga cgatctgggt ggcgatgccg gcgtggtcgg tgcccggctg
     3121 ccacagggtg ttccagccgc gcatgcgatg gtagcgggtg agcgcgtcca tgatcgtctg
     3181 gttgaaaccg tgacccatgt gcagggtgcc ggtgacgttg ggcggcggca gcaggatgca
     3241 gaaggattgg tcggcgggct tgctgcggtc gaggccggcg gcgaagtagc cgctcgattc
     3301 ccagtgctga taccagcggc gttcgatgtc cgccggttcg aaactcttgg ccagttccat
     3361 ggggtccgtc ttgtgaaggc aaaggacgga ttatagcgga tgcgctcccg gcgcccggcg
     3421 gcgcgggcgg ggcttattcc tcggtgtcga gggttttttc gatctgcgcg gtgagggtgg
     3481 gcagcagggt ggtgcgcatg tgttcgcggg cggcctcgcg caggcgttcg gcaaaggcgt
     3541 cgaactcgcg ggacatgatc tggggcagtt cggtggagac ccaggcgtcg atgctgcggg
     3601 tgatggcggc ttccaggtcg acgatgcgcg ccgtctggtc gtctcgggcg ttggggagcg
     3661 cgaccggcgt gatgtcgggt gggctgtcgt cgggcggtgc ttcctcgccg ggatagtcga
     3721 cgacgtcggt caggaccggc agatcgtccg gttcggcagg cgccgcgaag tgtccgtggc
     3781 ggcgcatgag ggcgtcggcc ttggcgagga ggtgttcgcc gtcctcgtct tcgagaggga
     3841 cttccaggac cgggcggtgg gaatcgctca tgcgccgctc cgctgggcga gatcgtgggc
     3901 gtggagggga aagccggcct gcttgtaggc gcggaagcgc tgacgcgcgg cgtggcgttc
     3961 gaattcgtcc tggccgacga tctcgacgac cagcccgaag cgttcgaaac ccggcggcag
     4021 ttcgccgccc aggttgatca gcacgtcctc gtgatgcagg gcgtccaggt gcgtgccgat
     4081 caccaccggc gtctcggccg ccagggggga ttccgcgccc acgtggggca cgaaggcgag
     4141 gggctggaag gaccacagga gctggtcgaa gtggcgcgcc tgggcgccgt ccgtcgccag
     4201 cacggagatc cgccgaccct ggccccaggc tttggcggtg acggagcagg ccacccgaag
     4261 gcggtccggc gcgttgtggt agaagcggat ctcgggcatt ggcggcgccc cgttcaggcg
     4321 cctgcgcgac gggccaggaa ttccaccagc agcggcaccg ggcggccggt ggcgcccttg
     4381 tcggcaccgg acttccaggc ggtgccggcg atgtccaggt gggcccatgt gtaggccttg
     4441 gtgaagcgcg ccaggaaaca ggccgcggtg atggtgccgg cgcccttgct gccgatgttg
     4501 ggaatgtcgg cgaagttgct cttgagcatg tcctggtatt cgtcccacag gggcagctgc
     4561 caggccttgt cgccggcaga ctggccggcg gcgagcagtt cggcggcgag ggcgtcgtcg
     4621 ttggcgagga ggccggaggt caggttgccc agggcgatga tgcacgcgcc ggtgagggtg
     4681 gcgatgtcca ccacggcggc gggttcgtag cgctcggcgt aggtgagggc gtcgcagagg
     4741 atcaggcggc cctcggcgtc ggtgttgagc acctcgatgg tctggc
//
LOCUS       DAOQPA010000185         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11295_length_4745_cov_17.594670, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000185 DAOQPA010000000
VERSION     DAOQPA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_4745_cov_17.594670"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKX01_16340"
     CDS             <1..222
                     /locus_tag="PKX01_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77755.1"
                     /translation="GSTGLVIERMSREIGRMASSPALSPSTRSAASTMVGLTSLGGGG
                     GGAVDGPLLALLGAAASLAIAARRGVRRT"
     gene            255..602
                     /locus_tag="PKX01_16345"
     CDS             255..602
                     /locus_tag="PKX01_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HMZ77756.1"
                     /translation="MRYTGSCHCGHVAFEAEGEITGLLECNCSICRRKGALMWFVPRS
                     AFRLLTPEENAATYTFASGRIHHRFCPRCGIQPYGEGDDPTHGPMVAINARCLDNVDL
                     AALPVRHFDGASL"
     gene            complement(662..2023)
                     /locus_tag="PKX01_16350"
     CDS             complement(662..2023)
                     /locus_tag="PKX01_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77757.1"
                     /translation="MVDEAGTRGGAGPDGATQRIGEAAEKGARGDVWGADGVNGARAG
                     DVELARWLDPIHRLSELLHAAACGLAGVCHPRTQVALAVRVMRVQYLLRERTGDLEAA
                     LALFEGGGRRFMSAVDAALKDAAAGYPEDGLLRLARCTPHLDCMRQGVQGLCGELDTV
                     REAVDAALAAVAEVYAPQDADWSREAHTRGRVFECRADVAPGWADDESGAGAARLTSE
                     RRPIRCDAEEASAPVPVPQAARPGPAVGAGEGPAPPRDGPSGALPAVCEAGAEGGDGG
                     AWAFAEAAHEAALNADLNALAMAADGAAVEAAAARLLCHARVELARMAGMLTRLGGHG
                     QVLIEGGRRLAGETVPGWIRGDGPHGAGDTDARAGDERARAEARVRAGWHAQMQAVQA
                     LRGGLTGLLREMGLRYAFNPLPEAGRREAVAWARAWLGAADAIRRGEVAPGGEAIPPA
                     RLT"
     gene            complement(2080..3042)
                     /locus_tag="PKX01_16355"
     CDS             complement(2080..3042)
                     /locus_tag="PKX01_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77758.1"
                     /translation="MKRSLHGLVGLWLLLWGLAWADGAQLQRVTRVFDALHQSEAALA
                     AGNTSRAQALLTDIRSAADGLRQDTETYVNRARGAADRRQADVLRTVQEIDETFRLEG
                     EADREMRDRQARIADLDVQLAEVARVRVALDARMAELRQEGRTRDECRAQPLEGMFYS
                     WECWRLSFADVFANRWKALNNEAADNQRQRIQIEMDRQGAGNERDRAQRSLDMLRARK
                     ADLQARRSQLDREQNTLRAAVTRLTDAVQFWRDLGVLVQSNIGTVAMLQDSVQRLAQR
                     ASTQAPTPVFDRYDAETVRSLEDTLKDFARSIDDGRNILLADGA"
     gene            complement(3141..>4745)
                     /locus_tag="PKX01_16360"
     CDS             complement(3141..>4745)
                     /locus_tag="PKX01_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77759.1"
                     /translation="GRMIPILRGAFKDLYGFYEADCVGRLTGCEAVATSMATTAENLK
                     STFRGLADEAQKIAEDTTQTRDLRRKAKEAAQARQKEIKADLEEKERLKASLAEQIPQ
                     VKGWYEEAKAAQNTADGRMFALAIVGSITKALGDGLAAGLQFKTAPVTAGTQLATAIV
                     KNTAVKEPDEKNKKTDDKAKATSETLTAQAAYRVAKDALDQAEAKLTAAQTATKTAQG
                     KLDKLEEPYIEARDAYEAARKKKPKDKKDEAAKKEAFEELEEPYKEAEDAVEDAEAAE
                     EAAQKAVDAAKVKESTAKAVMEDAAAKAPLAGVAEGLRSAGASAQEMGASYADIAANY
                     AKEKAKYLDMLMDLQKQEREALGAIAKFAVQMQTEKDTEQLEQAAVESLHIAVGVLKY
                     VVVILQDVCQFWTQMAAACRRLASADLRTDIEVYMRRTKEERVKEYSAEDFKLRMLAV
                     AAQWRALKRVATEYRAATMQVFDKLGVTYRDNLPIEQARAEAKRLAQLLDKDVKDEIK
                     KTDDATQQIAAAKAEADKLIAKAAAA"
BASE COUNT          566 a         1889 c         1499 g          791 t
ORIGIN      
        1 ggatcgacgg ggctggtgat cgagaggatg tcgcgcgaga tcggcagaat ggcgtcatcg
       61 ccagcgctgt ctccgtcgac aaggtccgcg gcctcgacga tggtcggctt gacttcgctt
      121 ggtggcggcg gcgggggcgc ggtggatgga ccgctgctcg ccctcctggg cgccgccgcc
      181 agcctcgcca tcgcggcccg ccgcggcgtc cggcgcacct gacccctgcc tcctccaacc
      241 cgaaaggatc tcccatgcgc tacaccggaa gctgtcactg cggccacgtc gccttcgaag
      301 ccgaaggcga gattacgggt ctcctggaat gcaactgctc catctgccgg cgcaagggcg
      361 cgctgatgtg gttcgtgccg cgcagcgcct tccgcctcct cacgccggag gagaacgccg
      421 ccacctacac cttcgcttcc ggccgcatcc atcaccgctt ctgcccccgc tgcggcatcc
      481 agccctacgg cgaaggcgac gacccgaccc acggccccat ggtcgccatc aacgcgcgct
      541 gtctcgacaa cgtggatctg gcggccctgc cggtccgcca cttcgacggc gcatccctgt
      601 agcgcacaga gggccatcgc ggccctcgcc catcccggcg cgagcgcgtc gcccggcgcc
      661 ctcaggtcag gcgcgcaggg ggaatggcct cgccgccggg cgctacctca ccgcgccgga
      721 tcgcgtccgc ggccccgagc cacgcccgag cccaggccac cgcctcccga cgcccggcct
      781 ccggcagcgg attgaacgca tagcgcagcc ccatctcgcg cagcaggccg gtgagccccc
      841 cgcgcagcgc ctgcacggcc tgcatctgcg cgtgccaacc cgcgcggacc cgcgcctcgg
      901 cccgcgcgcg ctcatcgccc gcacgcgcgt ccgtgtcgcc cgccccgtgg ggcccatcgc
      961 cacggatcca gccgggcacg gtctcgcccg ccagacggcg accgccctcg atcaatacct
     1021 gtccgtgtcc gcccaggcgg gtcagcatcc cggccatccg cgccagctcc acccgggcgt
     1081 ggcacagcag acgcgccgcg gccgcctcga ctgcggcgcc gtcggccgcc atcgccagcg
     1141 cgttcagatc ggcgttcagc gccgcctcat gcgcagcctc cgcaaacgcc cacgcccccc
     1201 catcgccgcc ctcggcgccc gcttcgcaca ccgcgggcaa cgccccggag ggtccgtcgc
     1261 gtggaggcgc cggtccttcg cccgcgccga cggcgggccc gggccgggcc gcctgcggga
     1321 caggcaccgg tgcgctcgcc tcctccgcgt cacatcggat cggccgccgc tccgacgtca
     1381 gccgggcagc gcctgcgcct gattcatcgt ccgcccatcc gggcgccacg tcggcgcggc
     1441 attcaaacac gcggccacgg gtgtgagcct cgcgcgacca gtctgcgtcc tgcggagcat
     1501 agacctccgc caccgctgcc agcgccgcat ccacggcctc gcgcacggtg tccagctccc
     1561 cgcagaggcc ctgcacgccc tgacgcatgc agtcgaggtg gggcgtgcag cgcgccaggc
     1621 gcagcagccc gtcctccgga tagccggccg ccgcatcctt cagcgccgcg tcgacagcgc
     1681 tcatgaatcg ccggccgccg ccctcgaaga gggccagcgc ggcctccaga tcgccggtcc
     1741 gctcgcgcag cagatactgc acccgcatca cgcgcaccgc gagggcgacc tgtgtgcggg
     1801 gatgacagac cccggcgagc ccgcaggccg cagcgtggag caactcgctc aggcgatgga
     1861 tggggtccag ccagcgcgcg agctccacgt cgccggcccg cgcaccgttc acgccgtccg
     1921 cgccccagac gtcgccccgc gcgcccttct ccgccgcctc gccgatgcgc tgcgtcgccc
     1981 cgtcaggccc agcgccgccc cgcgtgcccg cctcatccac catggcgatc cacgatcctc
     2041 ccggcggccg gggcacggcc tcgtgcccct cgatcccgct caggcgccat cggccagcag
     2101 gatgttgcgg ccatcgtcga tgctccgggc gaagtccttc aaggtgtcct ccaggctgcg
     2161 caccgtctcc gcgtcgtagc ggtcgaacac cggcgtcggg gcctgcgtgc tggcgcgctg
     2221 ggccaggcgc tgcaccgaat cctgcagcat cgccacggtg ccgatgttgg actggacgag
     2281 cacccccagg tcgcgccaga actgcacggc gtccgtcagg cgcgtcaccg cggcccgcag
     2341 ggtgttctgc tcccggtcca gctggctgcg gcgggcctgc agatctgcct tgcgggccct
     2401 cagcatgtcg agggaacgct gcgccctatc gcgctcgttg ccggccccct ggcgatccat
     2461 ctcgatctgg atccgctgcc gctggttgtc ggcggcctcg ttgttgagcg ccttccagcg
     2521 attggcaaac acatcggcga agctcagccg ccagcactcc cagctgtaga acatcccctc
     2581 cagcggttgc gccctgcatt catcgcgggt ccgcccctcc tggcggagct cggccatccg
     2641 cgcgtccagg gcgacgcgca cgcgcgccac ctcggcaagc tgcacgtcca ggtccgcgat
     2701 gcgcgcctgc cggtcccgca tctcccggtc ggcctcgcct tcgaggcgga aggtctcgtc
     2761 gatctcctgg accgtgcgca gcacatccgc ctgacgccgg tcggccgcac cgcgggcgcg
     2821 attcacatag gtctcggtgt cctgacgcag accgtccgcg gcgctgcgga tatccgtcag
     2881 cagcgcctgg gcgcgggagg tgttgcccgc cgccagcgcc gcttccgact ggtgcagggc
     2941 gtcgaagacc cgtgtgaccc gctggagctg cgccccatcc gcccaggcca ggccccacag
     3001 cagcagccac agcccgacca gtccgtgcaa gcttctcttc atcaccatgt ctcccgcccg
     3061 cgcccgaccc acgccacctc gcggcggccc gtcacggccc cgcgaagtgg cgcatcccgg
     3121 gcgcaggact gtgccgggtg tcaggcggcc gccgccttgg cgatcagctt gtccgcctct
     3181 gccttggctg ccgcaatctg ctgggtggcg tcatccgtct tcttgatctc gtccttcacg
     3241 tccttgtcca gcagctgggc gagccgcttg gcctcggcgc gggcctgttc gatgggcagg
     3301 ttgtcccggt aggtgacgcc cagcttgtcg aagacctgca tcgtcgccgc ccggtattcc
     3361 gtcgccaccc gtttcagggc gcgccactgg gcggccaccg cgagcatgcg cagcttgaaa
     3421 tcctcggcgg agtattcctt cacccgctct tccttggtgc ggcgcatgta cacctcgatg
     3481 tcggtgcgca ggtccgcact cgccagccgc cggcaggccg ccgccatctg ggtccagaac
     3541 tggcagacgt cctgcaggat caccaccacg tacttgagca cgcccaccgc gatgtgcagc
     3601 gattccaccg ccgcctgttc gagctgctcg gtgtccttct cggtctgcat ctgcacggca
     3661 aacttggcga ttgcgcccag cgcctcccgc tcctgcttct gcaggtccat cagcatgtcc
     3721 aggtacttgg ccttttcctt ggcgtagttg gcggcgatgt ccgcgtagga ggcgcccatc
     3781 tcctgggcgc tggcacccgc cgaacgaagg ccctccgcca cccccgcgag gggcgccttg
     3841 gcggccgcgt cctccatcac cgccttggcc gtgctctcct tcaccttggc ggcgtccacc
     3901 gccttctggg ccgcctcctc ggccgcctcg gcgtcttcca ctgcgtcctc ggcttccttg
     3961 tagggctctt ccagctcctc gaaggcctcc ttcttggcgg cctcgtcctt cttgtccttg
     4021 ggcttcttct tgcgcgccgc ttcataggcg tcgcgggcct cgatgtaggg ctcctccagc
     4081 ttgtccagct tgccctgggc cgtcttggtc gccgtctggg ccgcggtgag cttggcctcc
     4141 gcctggtcca gggcgtcctt cgccacccgg taggcggcct gggccgtcag ggtctcggag
     4201 gtggccttgg ccttgtcgtc ggtcttcttg ttcttctcgt cgggctcctt gaccgccgtg
     4261 ttcttgacga tggccgtcgc cagctgcgtg cccgccgtca ccggcgcggt cttgaactgg
     4321 agcccggccg ccagcccgtc ccccagggcc ttggtgatgc tgcccacgat ggccagggcg
     4381 aacatgcgcc cgtcggcggt gttctgggcc gccttggcct cttcgtacca gcccttcacc
     4441 tgggggatct gctccgcgag ggaggccttc aggcgctcct tttcttccag atcggccttg
     4501 atctccttct ggcgcgcctg ggcggcctcc ttcgccttgc gccgcagatc ccgcgtctgg
     4561 gtggtgtcct cggcgatctt ctgggcctcg tcggcgagcc ccctgaaggt gctcttgagg
     4621 ttctccgccg tcgtcgccat gctggtcgcc acagcctcgc agccggtcag gcggcccacg
     4681 cagtccgctt cgtagaagcc gtacagatcc ttgaaggcgc ccctcaggat cgggatcatc
     4741 cgccc
//
LOCUS       DAOQPA010000186         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11370_length_4724_cov_21.644678, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000186 DAOQPA010000000
VERSION     DAOQPA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11370_length_4724_cov_21.644678"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..926)
                     /locus_tag="PKX01_16365"
     CDS             complement(213..926)
                     /locus_tag="PKX01_16365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HMZ77760.1"
                     /translation="MLLTDQSLSEAELDDLDAFLMSDAMPEGGMDISTLDGFLAAVVL
                     NPDIVLPSQWLPWAWDMEDAEDEPVFKNAEEAERITGLIMRHYNMVIQGIRHGTFDPL
                     LFELRQDDGSEFFDAEGWCEGFMLGVSVFIDAWRPVLEQHMELVAPMVLLGTDRGWKM
                     LEESGNNKEATRQAYESIPAAVIAISEHFRPQREAMGRSRTGAAPTTRSAPKVGRNDP
                     CSCGSGKKYKKCCGIETLH"
     gene            complement(1007..3202)
                     /gene="mobH"
                     /locus_tag="PKX01_16370"
     CDS             complement(1007..3202)
                     /gene="mobH"
                     /locus_tag="PKX01_16370"
                     /inference="COORDINATES: protein motif:HMM:NF041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HMZ77761.1"
                     /translation="MFSWLKRKAPSTPRPRVHEPIYHWSDDEIPRYPPFMKGLPAVPP
                     DRLLTTQSELLERVADTAIATPQIFARFYVPAIGRFASFAHLLPASQSHHHRGAGGLL
                     RHSIEVGLWALQSADKVLLDAAKTPSQRREMEPRWQLAVFLAALCHDAGKPVTDLIVT
                     NRDRTAIWKPIKEDLYAWATRSGIDAYYLDWREGRSRQHTALANLIADRIIGTDALEW
                     IEEGGTELIVWLLESLNGNPGPTNLVYDLVVKADQSSVERDLRTLGVAMAGYDLGVPI
                     ERHLTDVMRRFVKEGTWLVNQPGARLWNIDGTLYLVWPSAGEEIARQVREDGIPGIPR
                     TPDGILDMLVERQIAFVREGTTPGGRLWKIAPAVLAEKIPDIRLPAIRLMDEAMVSSV
                     PIPPIAGVVDDGEGDLQTSPSGESPPAADRQRDVRIASDADAMQEQAPSAEPHPGLGV
                     LRKTPAVVLDGAVGEALKALAQDLESGDKRWGEDAEFDAGQQLLLRWPDAFSGYGLTA
                     KGILDELTRREWLWIDPMAPLKKVLDAEIGGRPVKAIRLERAISQVLIREPGAIAGAE
                     GSQGRAPSESTPPHKTGQERMADGPQLGHGPDGGDPMAEPGQTRSPKRAVRSSRLDEA
                     AECGPSTQHAANSCRHSHGANPDDTGPERRKGAPQTATQPSLSEILKVLNGYPGTTQA
                     DGWRTLPKSEALTACRNHGLRVTHGWLAKLGQEEPGRFAVDGVVVKYRP"
     gene            complement(3212..3709)
                     /locus_tag="PKX01_16375"
     CDS             complement(3212..3709)
                     /locus_tag="PKX01_16375"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HMZ77762.1"
                     /translation="MLDALHIHVSRRAPVYLWMLVGIAAFGRWFALTLNISDSLPGTV
                     FLVQKGSKPDKGQLAAFRYAGGGPYERGVLFLKQMLGVPGSLVIGMDIGSGYRDYFVD
                     GQYAGRAKPRSKTGMHLDPGPSGVIPAGHYYMAAPNPDSLDSRYALVGWVAEDQIVGR
                     AFRIF"
     gene            complement(3786..4253)
                     /locus_tag="PKX01_16380"
     CDS             complement(3786..4253)
                     /locus_tag="PKX01_16380"
                     /inference="COORDINATES: protein motif:HMM:NF017124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="HMZ77763.1"
                     /translation="MVALCELESLRLAHDLIRAGLRLSIVRSLTKIGTRSLRQWWKDI
                     HGVRPSNGKLPETVLSFIKDKDSAAMVSAFGAFHNRLHGTTLSPENLLATWQEFQRIC
                     GPLDINAAYFAVRDIRARIVMLSHCDVCHANFIYDAGSKHTDQCPFCATKVVA"
     gene            complement(4253..4528)
                     /locus_tag="PKX01_16385"
     CDS             complement(4253..4528)
                     /locus_tag="PKX01_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77764.1"
                     /translation="MNDIISINRQFLTMAREAAKSKSGEIVTGLSRPVLDKLANLSLD
                     QIEGIAQGAAVSLISLRLTEAELNRLVSLQNSQRTAYSLAVVASTER"
     gene            complement(4525..>4724)
                     /locus_tag="PKX01_16390"
     CDS             complement(4525..>4724)
                     /locus_tag="PKX01_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77765.1"
                     /translation="MDTGRLSELLLRHPKHALPPMAETIADFIVRFLTQKRPFSVCFV
                     IDDIPNDRYISPSYRISGKAS"
BASE COUNT          854 a         1487 c         1444 g          939 t
ORIGIN      
        1 tctcatgacc ggtatgcttg gccacctccg cgttgaagca acggctgcaa aggtctcgat
       61 atccctgccc cgtcgtaccg tagctgacga tgtcgtaatc gggcgtgctc tcgccacaac
      121 cttcgcattg tacttgtcgc atgccgatca atcctcttgc cacggccact cgttggccat
      181 tgcggcacag tgcttcggaa cgcttgcggc catcagtgca atgtctcgat accgcagcac
      241 ttcttgtact tcttgcctga accacaggaa caagggtcgt ttcgtcccac ctttggggcg
      301 ctacgcgtgg tcggtgccgc gccggttctg gagcgaccca tcgcctcccg ttgcggcctg
      361 aaatgctcgc tgattgcgat caccgccgcc ggtatcgact cataggcctg ccgcgtggcc
      421 tccttgttgt tcccgctttc ttcgagcatt ttccaacccc gatcggtgcc caggagcacc
      481 atgggggcca ccagctccat gtgctgctca agaacgggcc gccacgcatc aatgaagacc
      541 gatacgccaa gcatgaagcc ttcgcaccag ccctcggcat cgaagaactc gctcccgtcg
      601 tcctggcgca actcgaacag caatgggtcg aaggtgccgt ggcggatgcc ctggatgacc
      661 atgttgtagt gacgcatgat gagacctgtg atcctctcgg cctcctcggc gttcttgaat
      721 accggctcgt cctcagcgtc ttccatatcc caggcccaag gcagccactg gctcggcaat
      781 acaatgtcgg gattgaggac aacagcggcg agaaagccat ccagcgtcga aatgtccatg
      841 ccgccctcgg gcatggcgtc cgacatcagg aaggcgtcca ggtcatcgag ttcggcctcg
      901 gagagcgatt ggtcggtgag gagcatgagc gagcgatacc aagcaaaatt gcatcttatc
      961 ttgccgccgg attgcaggcc accgcgcaaa gcgatccggc cagccctcag ggacgatact
     1021 tgacgacgac accgtcgacg gcgaatcggc ccggctcttc ctggccgagc tttgccagcc
     1081 atccgtgcgt caccctgagc ccatgattcc tgcaggcagt cagagcttca gatttcggta
     1141 gcgtgcgcca tccgtcggct tgtgtcgtcc caggatagcc gttgagcacc ttcagtatct
     1201 cgctgagcga gggctgagtg gctgtttgcg gcgccccttt tcgcctctct ggtcccgtgt
     1261 catcggggtt ggcgccatgg gagtggcggc agctgttagc agcatgctgc gtggatggtc
     1321 cacattcagc ggcttcgtca agccgtgacg atcgcacagc tcgcttcggc gatctcgtct
     1381 ggcctggctc cgccatgggg tctcccccgt ccgggccatg tcccagttgc gggccatccg
     1441 ccatccgctc ctggcctgtc ttatggggtg gcgtcgattc cgatggcgcc ctgccctggc
     1501 ttccctcggc ccctgcaata gcgcctggct cacgaatcag gacctggctg atcgcgcgct
     1561 ccaaccggat cgccttcacc ggcctgccac cgatctccgc atccagaacc ttcttcaggg
     1621 gcgccatcgg gtcgatccag agccattcgc gccttgtcag ctcgtcgaga atccctttgg
     1681 ccgtgagtcc atatccgctg aacgcatcgg gccatcgaag gagcagttgc tgccccgcat
     1741 cgaactcggc gtcttctccc caccgcttgt cgccggactc gagatcctgc gcgagtgctt
     1801 tcagggcttc gccgacggca ccatccaata cgaccgccgg cgtcttccgc aggacgccga
     1861 gcccaggatg cggttcagct gacggagctt gttcctgcat ggcatctgcg tcagaagcga
     1921 tgcgcacgtc tcgttgtcgg tcggccgctg gtggcgactc cccactcggg gacgtctgaa
     1981 ggtcgccctc accatcatca acaacccctg cgatgggagg aatcgggacg gacgacacca
     2041 tagcctcgtc catcagcctg atggccggaa gtctgatgtc agggatcttc tcggccaaga
     2101 ccgcgggcgc gatcttccag agccggcctc ctggcgttgt tccctcccga acgaatgcaa
     2161 tctgccgctc caccagcatg tccaggatgc catccggcgt tcttggtatg ccgggaatgc
     2221 catcctcgcg aacctgcctg gcgatctcct ctccggccga gggccagacc aagtagaggg
     2281 tcccatcgat gttccacagt cgcgcgccgg gctgattgac cagccaagtg ccctccttga
     2341 cgaaccgacg catgacatcc gtgagatggc gctcgatcgg gacacccaag tcgtacccgg
     2401 ccattgccac cccgagcgtt ctcaaatcgc gctcgacgct cgattggtca gccttgacga
     2461 cgagatcgta gactaggttc gtggggccag gattgccatt gagcgactcc agcagccaga
     2521 cgatgagttc cgtgcccccc tcttcgatcc attcgagcgc atcggtaccg atgatccgat
     2581 cggcgatgag gttggcgagc gcagtgtgct gccgggatcg gccttcgcgc caatcgagat
     2641 aataggcgtc gattcccgac cgtgtggccc aggcatagag atcttccttg atgggcttcc
     2701 agatcgccgt gcggtcccgg ttggtcacga tcaggtcggt cacgggcttg cccgcgtcat
     2761 ggcagagcgc tgccagaaac acggcgagct gccagcgcgg ctccatctcg cgcctttgcg
     2821 acggagtctt ggcggcgtcc agcagcacct tgtctgcgga ttgcagggcc caaaggccca
     2881 cttcgatgga gtgccgaaga aggccgccgg cgccgcggtg atggtgggac tgggatgccg
     2941 gcaacaggtg cgcgaacgac gcgaaccgtc cgatggcggg cacgtagaag cgcgcgaaaa
     3001 tctgcggcgt ggcgatcgcc gtgtcggcga ctcgttcgag caattcgctc tgggtggtga
     3061 ggagccggtc cgggggtacc gccggcagcc ccttcatgaa tggcggatag cgtgggatct
     3121 cgtcatcgga ccagtggtag atcggttcat ggactcttgg tcgtggcgtt gagggggctt
     3181 tcctcttgag ccaggagaac atccgcgatc ctcagaatat ccggaacgcg cggccgacga
     3241 tctggtcttc ggctacccac ccgaccaggg cgtagcggga atccaggctg tcagggttgg
     3301 gagccgccat gtagtagtgg ccggcgggaa tgacgccgga tggtccgggg tcgaggtgca
     3361 tgccggtctt ggacctcggc ttggcccggc ccgcgtattg cccgtctacg aaatagtccc
     3421 gatagccgct gccgatgtcc atgccgatca ccaacgatcc cggaacgccc agcatctgct
     3481 tcaggaacag gactccgcgt tcgtaggggc cgccgccggc atagcggaag gcagccagct
     3541 ggcccttgtc aggcttggat cccttctgga cgaggaaaac cgttcccggg agggagtccg
     3601 agatgttgag ggtcagggcg aaccaacgcc cgaaggccgc gatgccgacg agcatccaga
     3661 gatagaccgg cgcgcggcgc gagacgtgga tatggagagc atcgagcatc caccgatgct
     3721 acgcggcggg gttggtcatt tcggacccga aaagcgtctc cgtggcgccc gtttgaaggg
     3781 tgagcctacg caaccacctt ggtcgcgcag aacggacact ggtccgtgtg cttgctgccg
     3841 gcgtcataga tgaagttggc atgacatacg tcgcaatgcg agagcatcac gattcgggcc
     3901 ctgatgtccc gcaccgcgaa atacgcggca ttgatgtcga gcggaccgca gatccgctgg
     3961 aactcttgcc aggtggccag caggttttcg ggggacagcg tcgtcccgtg caggcgattg
     4021 tgaaacgcgc cgaacgccga gaccatggcc gccgagtcct tgtccttgat gaaggacagt
     4081 acggtctccg gcagcttgcc gttcgagggt ctgacgccgt ggatgtcctt ccaccattgg
     4141 cgcagcgaac gtgtcccgat cttggtcagg ctgcgaacga tcgacagacg gaggccggcg
     4201 cggatcagat catgggcaag gcgcaggctt tcgagctcgc atagcgcgac catcagcgct
     4261 ccgtcgatgc cacgaccgcc agtgaatagg ctgtcctctg gctattctgg agcgacacca
     4321 atcgattcaa ctcggcttcc gtcagtcgca agctgatcag gctgaccgcg gcgccctggg
     4381 cgatgccctc gatctgatcg aggctgaggt tggccagctt gtccagaacg ggtcgggaca
     4441 gcccggtgac gatctcaccg gacttggact ttgccgcctc cctcgccatg gtgagaaact
     4501 gccggttgat cgaaatgatg tcattcatga cgcttttccc gaaattcggt atgatggcga
     4561 aatatatcta tcattcggta tgtcgtcaat gacaaaacag accgaaaaag gacgcttttg
     4621 agtcaagaac ctgacaatga aatcggcgat cgtctccgcc attggcggaa gcgcgtgctt
     4681 gggatgacgc aggagcagtt cgctcaagcg accggtatcc atct
//
LOCUS       DAOQPA010000187         4699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11477_length_4699_cov_15.355082, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000187 DAOQPA010000000
VERSION     DAOQPA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11477_length_4699_cov_15.355082"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKX01_16395"
     CDS             complement(<1..334)
                     /locus_tag="PKX01_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77766.1"
                     /translation="MNTYFAALAVAMAVLTGFAGEAVAAPQAHRAAAPSKTAPKPVAR
                     VTPRPAPVKLAPKPVFRPAAQAQVRGKTAPARTGVRPAPAPVRAQARPAPVVKALARA
                     PAPVASARG"
     gene            537..2864
                     /locus_tag="PKX01_16400"
     CDS             537..2864
                     /locus_tag="PKX01_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HMZ77767.1"
                     /translation="MLLPIEQRIAAELGVQPRQIESAVQLLDEGATVPFIARYRKEVT
                     GGLDDTQLRTLEERLGYLRELEDRRAAVLASIDEQGKLTPALREAVEHADTKQRLEDL
                     YLPYKQKRRTKAQIAREAGIEPLALALLADPGLSPDAEAAAYLNTDAGFADTRAVLDG
                     ARQILMERFAEDATLIGTLRSYLGEHGVVVSSVVAEKEADPAAAKFRDWFDFREPLAT
                     LPSHRALALFRGRNEGVLRLQLGLDSDPTDGSTPGPNPCEARIAGHVGVRDQGRPADA
                     WLRETVRWAWSVKISLHLELELLGQVRERAEEEAIRVFGRNLKDLLLAAPAGARATLG
                     LDPGLRTGVKVAVVDQTGKLLDTATIYPHEPRRDWDGSLHTLSKLCEKHGVSLIAIGN
                     GTASRETDRLAADLIKLRPELHMTKIVVSEAGASVYSASELAAREFPDLDVSLRGAVS
                     IARRLQDPLAELVKIDPKSIGVGQYQHDVNQTRLARALDSVVEDCVNAVGVDVNMASA
                     ALLARISGLNTTLAANIVAHRDAHGPFPSRAALLDVPRLGPKTFEQAAGFLRIPNGSN
                     PLDASAVHPEAYPVVERILADIRKNARELMGDTRAIGQLKAERYTDERFGLPTVQDIL
                     RELDKPGRDPRPEFKTASFKDGVEDLKDLVPGMLLEGVVTNVTNFGAFVDIGVHQDGL
                     VHVSALADRFVKDPHSVVKAGQVVKVKVLEVDLPRKRIALTMRLADTPAAAARPAGER
                     REAPRNGPRPQAARPAREPAMAGSLATAFAKALKK"
     gene            2955..3320
                     /locus_tag="PKX01_16405"
     CDS             2955..3320
                     /locus_tag="PKX01_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HMZ77768.1"
                     /translation="MSTTVYGIKNCDTMKKAFAWLDEHGVAYTFHDYKKSGIDSARLH
                     AWSKAVGWKTLLNTRGTTWRKLPPERQALDTQSAAVALMGEFPSVIRRPVVETAGGQL
                     LVGFDPATFASFLGGEPRA"
     gene            3317..4201
                     /locus_tag="PKX01_16410"
     CDS             3317..4201
                     /locus_tag="PKX01_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HMZ77769.1"
                     /translation="MSRPDSFIQRFLLEELDIRGAVVRLDQVWQALLENRDYAPPLRD
                     LLGAMSAVTTVICGNLKQPGRITVQLQGEGPVRLLVVDCADTLNLRGYAKTRDDLSAP
                     LPPGGLGELVGDGQLLLSLDMPGLARPYQSYVPLEGDSVAEVFEHYLTQSEQQPALLR
                     TASSGTVAACLFLQKLPDADLKDPDGWQRVHHLVATLRNEELLALPPEALLTRLFHEE
                     QVRLFDPRDVTHDWPLDWEKVRRMLRSLGRDELDKIIAEHGEIHIHDDLSNHEYRLSA
                     ADVAELFAEEPPEQRTLH"
     gene            4344..>4699
                     /locus_tag="PKX01_16415"
     CDS             4344..>4699
                     /locus_tag="PKX01_16415"
                     /inference="COORDINATES: protein motif:HMM:NF017059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HMZ77770.1"
                     /translation="MSDPLLQSKLIEFKGATLPVMTAHLRDTDAVRLADALHMMLGGM
                     PDFFAGEPAILDLAGLTTVPERIDWTALLSMLRRYQVQPVAVRHLPAGLEDGARRAGL
                     ALLGPESAVTERPVARP"
BASE COUNT          761 a         1817 c         1458 g          663 t
ORIGIN      
        1 gtccacgggc gctcgccacg ggggccggcg cacgggccag cgccttgacc accggcgcag
       61 gccgagcctg ggcacgaacc ggcgcgggcg ccggacggac ccccgtacgg gcgggtgcgg
      121 tcttgccacg gacctgggcc tgcgccgccg gacggaacac cggtttgggc gcaagcttca
      181 ccggggcggg acggggcgtc acccgggcga cgggcttggg ggccgtcttg gacggcgcgg
      241 ctgcacgatg cgcctggggg gccgcgacgg cttcgccggc aaagccggtc agcaccgcca
      301 tggccaccgc caacgctgca aagtaggtat tcatggaatg ttttctcctg ctggaccggg
      361 ccgccggaag gggcgacgcg gcggactgtg cgcgaccggc gcgctcgcgg gcatgggcat
      421 gccgtggaaa accgggggat tctaccggaa gtttggaccg ttatcggcca cgatcggact
      481 tgcgggcgac ggcgcctttc tgcaccatgc gccctttttt cgcaccgggt ccacccatgc
      541 ttctgcccat cgaacaacgc attgccgccg aactgggtgt acagccccgc cagatcgaat
      601 ccgccgtcca gctcctcgac gaaggcgcca ccgtgccctt cattgcccgc taccgcaagg
      661 aagtcaccgg cggcctggac gacacccagc tgcgcacgct ggaggagcgc ctcggctacc
      721 tgcgcgaact ggaagaccgc cgcgccgcgg tgctcgccag catcgacgaa cagggcaagc
      781 tcacccccgc actgcgcgag gccgtggaac acgccgacac caagcagcgc ctggaagacc
      841 tttacctgcc ctacaagcag aagcgccgca ccaaggccca gatcgcccgc gaggccggca
      901 tcgagccgct ggcgctggcc ctgctggccg acccgggcct gtcgccggac gcggaagccg
      961 ccgcctacct caacaccgac gccggcttcg ccgacacccg ggcggtgctc gacggtgccc
     1021 gccagatcct gatggagcgc ttcgccgagg acgccaccct cattggcacc ctgcgcagct
     1081 acctgggcga gcacggcgtg gtcgtctcca gcgtcgtcgc cgaaaaggaa gccgatcctg
     1141 ccgccgccaa gttccgcgac tggttcgact tccgcgagcc cctcgccacg ctgccctccc
     1201 accgcgccct ggccctcttc cgtggccgca acgaaggcgt gctgcgcctg cagctgggcc
     1261 tcgattccga tccgaccgac ggcagcacgc ccggccccaa cccctgcgag gcacgcatcg
     1321 ccggccatgt gggcgtgcgc gaccagggcc gcccggccga cgcctggctg cgggaaaccg
     1381 tgcgctgggc atggagcgtc aagatctccc tgcatctgga actggagctc ctcggccagg
     1441 tgcgcgagcg cgccgaggaa gaagccatcc gcgtcttcgg ccgcaacctc aaggatctgc
     1501 tgctggccgc ccccgccggc gcccgcgcca ccctgggcct cgatccgggc ctgcgcaccg
     1561 gcgtcaaggt ggccgtcgtg gaccagaccg gcaagctcct cgacaccgcc accatctatc
     1621 cccacgaacc gcgccgcgac tgggacggca gcctgcacac cctttcgaag ctatgcgaaa
     1681 agcacggcgt gtcgctgatc gccatcggca acggcaccgc cagccgtgag accgacaggc
     1741 tcgccgccga cctcatcaag ctgcgccccg agctgcacat gaccaagatc gtcgtctcgg
     1801 aagccggcgc ctcggtgtat tccgcgtccg aactggccgc ccgggaattt cccgacctgg
     1861 acgtgagcct gcgcggcgcc gtctccatcg cccgccgcct gcaggatccg ctggccgagc
     1921 tggtcaagat cgaccccaaa tccatcggcg tgggccagta ccagcacgac gtgaaccaga
     1981 cgcgcctggc ccgtgccctg gattcggtgg tcgaggactg cgtgaacgcc gtcggcgtgg
     2041 atgtgaacat ggcgtctgcc gcgctgctgg cgcgcatctc gggcctcaat accacgctcg
     2101 ccgccaacat cgtcgcccac cgggacgccc atggcccctt tccgagccgc gccgcgctgc
     2161 tggacgtccc gcgcctcggc cccaagacct tcgagcaggc cgccggcttc ctgcgcatcc
     2221 ccaatggcag caaccccctc gacgcctccg cggtgcaccc ggaagcctac ccggtggtcg
     2281 agcgcatcct cgccgacatc cgcaagaacg cccgcgagct gatgggcgac acccgcgcca
     2341 tcggccagct caaggccgaa cgctacaccg atgagcgctt cggcctgccc accgtgcagg
     2401 acatcctgcg ggaactcgac aaacccggcc gcgacccgcg cccggagttc aagactgcca
     2461 gcttcaagga cggcgtggag gacctgaagg acctcgtccc cggcatgctg ctggaaggcg
     2521 tggtcaccaa cgtcaccaac ttcggcgcct tcgtggacat tggcgtgcat caggacggcc
     2581 tggtgcacgt ctccgccctg gcggatcgct tcgtgaagga cccccacagc gtggtcaagg
     2641 ccggtcaggt ggtgaaggtc aaggtgctgg aggtggatct gccgcgcaag cgcatcgccc
     2701 tcaccatgcg cctggcggac acccccgccg ccgctgcacg ccccgccggc gagcgtcgcg
     2761 aggcgccccg caacggcccg cgcccccagg ccgcccggcc ggcccgcgaa ccggcgatgg
     2821 ccggctccct cgccacggcc ttcgccaagg cgctcaagaa ataggcccgg cctgccgccg
     2881 ggccggctag aatccggtcc atctccttga ttttccagcg acgccccacc gggcgtcgcg
     2941 tcctgtccag ccccatgagc accaccgtct acggcatcaa aaactgcgac accatgaaga
     3001 aagccttcgc ctggctcgac gaacacgggg tcgcctacac ctttcacgat tacaagaaat
     3061 ccggcatcga cagcgcccgt ctccacgcct ggagcaaggc cgtcggctgg aagaccctgc
     3121 tcaacacccg cggcaccacc tggcgcaagc tgccgccgga gcgtcaggcc ctcgacaccc
     3181 agagcgccgc cgtcgcattg atgggcgaat tccccagcgt gatccgccgc ccggtggtgg
     3241 aaaccgccgg cggccagctg ctggtgggct tcgacccggc caccttcgcc agtttcctcg
     3301 gcggggagcc gcgcgcatga gccggcccga cagcttcatc cagcgcttcc tccttgaaga
     3361 actggacatc cgcggcgccg tcgtacgcct cgaccaggtg tggcaggccc tgctggagaa
     3421 ccgcgactac gccccgcccc tgcgcgacct gctgggcgcc atgagcgccg tcaccaccgt
     3481 catctgcggc aacctcaagc agcccggccg catcaccgtg cagctccagg gcgaaggccc
     3541 ggtgcgcctg ctggtggtgg attgcgccga caccctcaac ctgcgcggct atgccaagac
     3601 ccgcgacgac ctgagcgcgc ccctgcctcc tggcggactc ggcgaactgg tcggcgacgg
     3661 acaactgctg ctgtccctcg acatgcccgg tctggcgcgc ccctaccaga gctacgtgcc
     3721 cctcgaaggc gacagcgtgg ccgaggtctt cgagcactat ctcacccagt ccgaacagca
     3781 acccgcgctg ctgcgcaccg ccagttccgg caccgtggcc gcctgcctgt tcctgcagaa
     3841 gctgcccgac gccgacctca aagacccgga tggctggcag cgcgtccacc atctggtggc
     3901 caccctgcgc aacgaggaac tgctcgccct gccgcccgaa gccctgctca cccgcctctt
     3961 ccatgaagag caggtgcgcc tcttcgaccc gcgggacgtc acccacgact ggccgctgga
     4021 ctgggagaag gtgcgccgca tgctgcgcag cctgggccgt gacgaactgg acaagatcat
     4081 cgcggaacac ggcgaaatcc atatccacga cgacctctcc aaccacgaat accgactttc
     4141 cgccgccgac gtggccgaac tcttcgccga ggagccgccc gaacagcgca cccttcattg
     4201 aaatccgggg tttacttgcc ggaaaccctg ctgaaaagta gacttagccg gttcaggctc
     4261 ccgttttcca cctgcgggac ggcctcttac gcagccccgg cgcctgaccc gttcccgacc
     4321 ctctcacccg accgccttcc gccatgtccg accccctcct gcagagcaaa ctgatcgagt
     4381 tcaagggcgc caccctgccg gtcatgaccg cccatctgcg ggacacggac gccgtgcgcc
     4441 tggcggatgc cctgcacatg atgttgggcg gcatgccgga tttcttcgcc ggtgaaccgg
     4501 ccatcctcga tctggccggg ctcaccacgg tgcccgagcg catcgactgg accgccctcc
     4561 tcagcatgct gcgccgctac caggtgcagc ccgtggccgt gcgccacctg cccgccgggc
     4621 tcgaggacgg cgcgcgccgc gccgggcttg ccctgctcgg gcccgaatcc gccgtcaccg
     4681 agcgcccggt agcccgtcc
//
LOCUS       DAOQPA010000188         4679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11562_length_4679_cov_18.734862, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000188 DAOQPA010000000
VERSION     DAOQPA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11562_length_4679_cov_18.734862"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..607)
                     /locus_tag="PKX01_16420"
     CDS             complement(83..607)
                     /locus_tag="PKX01_16420"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ77771.1"
                     /translation="MTRSLPVLARSAVASLVLTAAHAALAAPALHVEESTTVAAPPAT
                     VWKLIGDYNALPAWHPAVARSDITKGTNNTRGAIRSLTTQDGAKIVEELLAYDGKARS
                     MTYRFLDTPMPITDYVATLAVMPEGHGSRIVWKSDFNRNASVDDAKAKGIITGIYQAG
                     LDALHKKLDTPAAH"
     gene            910..2607
                     /locus_tag="PKX01_16425"
     CDS             910..2607
                     /locus_tag="PKX01_16425"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ77772.1"
                     /translation="MGTESAGFRGKLWRGEGMCLIGMDVAAPEVDLVGFAIEVKSPGA
                     KTYTALRNRLNFAYDRPSVQAVTGARQFPSLLAPFQKFRWIHFPKDVQNGTYRYRLTK
                     IHMPAEGKLTKGTALELGIALDPETLPGFLNVAFTRNFASSQAFAERYGAQPALIPTK
                     ADDGLDFVKVAGDAYAWLGFEALALLNGTLDAALADPTCSVALLAYDLNEPDIVARLE
                     KLKGRLRAVIDNSKGHVEADSAESRAAARLAASAGAANVRRTRFKGLQHHKVIIVSRG
                     GTPVQVLFGSTNFSFRGLYIQANNMVSVTSPEVAGLFARMFDAAFADPDHFAAHPLAG
                     QWHEIRLAGRPPLSLCFSPHTDSDLSLGPVGQAIDGATSSVLFSIAFLNAIKSGPTRS
                     ALDRLAARPLFSYGVVDEDSGLALQKPDGSIGVVDFAYLAGKAPEPFRTEWSGGGGIN
                     IHHKFVVTDFNTPAASVFTGSSNLAPGGEAKNGDHLVRIQDPRVATAYAIEALRMFDH
                     LHFRVKMKAEQAAPRAPGALPPLVLQKPTALSGRPAWFEGAYRVGSQKARDRVLFSA"
     gene            complement(2733..3263)
                     /gene="lptA"
                     /locus_tag="PKX01_16430"
     CDS             complement(2733..3263)
                     /gene="lptA"
                     /locus_tag="PKX01_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HMZ77773.1"
                     /translation="MTSRIRPLLLALAALIATPFAHAEKADRSKPVNIEADKVTVDDK
                     NKIHIFEGHVILTQGTLTIRSDKLVVTQDMEGYQRGVATGGDGGLARFRQKRDNKDEW
                     VDGEGERLEHDARTELSQFFLRARVKSGQDEVRGQYIQYNGVTETYLVTNGPNATTLP
                     SQQSRVQVTLQPKKKD"
     gene            complement(3260..3841)
                     /gene="lptC"
                     /locus_tag="PKX01_16435"
     CDS             complement(3260..3841)
                     /gene="lptC"
                     /locus_tag="PKX01_16435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMZ77774.1"
                     /translation="MKDRVHSLFPAIVLGLLAGASIWLDRITQQDQTLPQDNSRHEPD
                     FIVEQVKLRRYDVTGKTQYVLVADKMTHFADDESSDLTRPRLDYLNRPEPMHLESDRA
                     LVSKEGEKVLMSGNVFARRAATPDKPESTLRSDRMTVWPDDEKMLTDTPVTLTQGQTV
                     VTAERMDADNIAGELHLHGQVRGTFYRKQASKP"
     gene            complement(3873..>4679)
                     /locus_tag="PKX01_16440"
     CDS             complement(3873..>4679)
                     /locus_tag="PKX01_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HMZ77775.1"
                     /translation="HIGRKLAATLASTGTPAYFVHAAEAAHGDLGMITPDDVLIAVSY
                     SGAGDELLRIVPLVKRSGARLIALTGNPDSPLAREADVHLDGAVKEEACPHNLAPTAS
                     TTASLALGDALAVALLDARGFGPEDFARSHPGGTLGRRLLTRVSDIMTSGPGLPRVTE
                     DATLGDALLEMTRGGLGMTAIVDGADQVVGIYTDGDLRRSLAGGCDFASARVADVMTR
                     NPRTIRAEALAVEAAEMMERLRISQLLVVDDARQLTGALNHIILMQAKVI"
BASE COUNT          634 a         1276 c         1912 g          857 t
ORIGIN      
        1 caatatgccg cgcagaccgt atagagccct gcgtcggtga ggtcggccgg ggcgggggcg
       61 atgcggcgcg gggccgtgtg gcttagtgtg cggccggggt gtcgagtttc ttgtgcaggg
      121 cgtcgaggcc ggcctgatag atgccggtga tgatgccctt ggccttggcg tcgtccacgc
      181 tggcgttgcg gttgaagtcg ctcttccaga cgatgcggct gccgtggcct tcgggcatga
      241 cggcgagggt ggcgacgtag tcggtgatgg gcatgggggt gtcgaggaag cggtaggtca
      301 tgctgcgggc cttgccgtcg taggcgagga gctcttcaac gatcttggcg ccgtcctggg
      361 tggtgaggct gcggatggca ccgcgggtgt tgttggtgcc cttcgtgatg tcggagcggg
      421 cgacggcggg gtgccaggcg ggcagggcgt tgtagtcgcc gatgagcttc cacacggtgg
      481 ccggcggggc ggcgacggtg gtgctctctt ccacgtggag ggccggggcc gcgagggcgg
      541 cgtgtgcggc ggtcaggacg aggctcgcca cggcgctgcg ggcgaggacg gggagggaac
      601 gggtcacggg aggtctcctt gttatgaatg aatgggctgc ggcagggcct gagccgtgac
      661 cgattctagg gatggggcgg cggcggggaa gaggccgacg gcaggtcggg catggggccg
      721 cgcgatgtcg ggccttttgc ccggcgaatg cggtgtggac cggtgcgatt gatgtccgcc
      781 agccggtgcg aaaggctgca ggcggtgcgc gaaggttctg caccgctgat tccgatgttg
      841 gcagcagggt ttttgccttc tatgctggat gggaatgggt cgagggggcg ggacatggac
      901 aatttttcgg tgggaactga atccgcgggc tttcgcggca agctgtggcg cggcgagggg
      961 atgtgcctga tcggcatgga cgtggcggcg ccggaggtgg atctggtggg ctttgccatc
     1021 gaggtgaagt cgccgggggc gaagacctat acggccttgc gcaatcgcct gaactttgcg
     1081 tatgaccggc cgtcggtcca ggcggtgacg ggggcccggc agtttccgtc gctgctggcg
     1141 ccgttccaga aattccgctg gatccatttt ccgaaggacg ttcagaacgg gacctaccgt
     1201 taccggctga ccaagatcca catgccggcg gagggcaagc tgacgaaggg gacggcgctg
     1261 gagctgggga ttgcgctgga tccggagacg ctgccgggtt ttctgaacgt ggccttcacg
     1321 cgcaactttg cgtcgtccca ggcctttgcg gagcgctacg gggcgcagcc ggcgctgatc
     1381 ccgacgaagg cggacgatgg gctggatttt gtgaaggtgg ccggcgacgc ctatgcgtgg
     1441 ctcggcttcg aggcgctggc gctgctgaac gggacgctgg acgcggcgct ggccgatccg
     1501 acgtgttcgg tggcgctgct ggcttacgat ctgaacgagc cggacatcgt ggcgaggctg
     1561 gagaagctga agggccggct gcgggcggtg atcgacaatt caaagggtca tgtcgaggcg
     1621 gacagcgcgg agagccgggc ggctgcgcgg ctggcggcat cggcgggggc ggccaacgtg
     1681 cggcggaccc gcttcaaggg gcttcagcat cacaaggtga tcatcgtgtc gcgcggcggc
     1741 acgccggtgc aggtgctgtt cgggtccacg aacttcagtt tccgcggcct ctacatccag
     1801 gcgaacaata tggtgtcggt gacgtcgccg gaggtggcgg ggctgtttgc ccgcatgttc
     1861 gacgcggcct tcgccgatcc ggaccacttt gccgcccatc cgctggccgg gcagtggcac
     1921 gagatccggc tggcggggcg tccgcccctg tccctgtgtt tctcgcccca cacggacagc
     1981 gatctgtccc tggggccggt ggggcaggcc atcgatggcg cgacatcgtc ggtgctgttc
     2041 tccattgcct tcctcaacgc gatcaaaagc gggccgaccc gctcggcgct ggatcggctg
     2101 gcggcgcggc ccctgttcag ctacggggtg gtggatgagg attcggggct ggccctgcag
     2161 aagccggacg gatccatcgg ggtggtggat tttgcctatc tggcgggcaa ggcgccggag
     2221 cctttccgca cggagtggtc gggcggtggg ggcatcaaca tccaccacaa gttcgtggtg
     2281 accgatttca acacgccggc ggcgtctgtc tttaccggtt cgtccaatct ggcgccgggt
     2341 ggggaagcga agaacggcga tcatctggta aggatccagg atccgcgggt ggcgacggcc
     2401 tacgccatcg aggccctgcg catgttcgat cacctgcact tccgcgtgaa gatgaaggcg
     2461 gagcaggccg caccgcgggc cccgggcgct ctgccgccgc tggtgctgca gaagccgacg
     2521 gccctgagtg gccggccggc gtggtttgag ggcgcctacc gggtcggttc gcagaaggcg
     2581 cgggaccggg tgctgttttc ggcgtgaagg gaaagatggg ggccgtcgcg ctgcgtggat
     2641 agggcgtcgg caggctacaa aaaaggaggc gcgggcctcc tttttttgtg ggtcgcacct
     2701 gtgcgccggg gcggtgcggc ggcgccgggc gttcagtcct tcttcttcgg ctgcagggtg
     2761 acctgcacgc ggctctgctg gctgggcagg gtggtggcgt tggggccgtt ggtgacgagg
     2821 taggtctcgg tgacgccgtt gtactggatg tactggccgc gcacttcgtc ttgaccgctc
     2881 ttgacgcggg cgcgcaggaa gaactgggag agttcggtgc gggcgtcgtg ttcgaggcgt
     2941 tcgccttcgc cgtcgaccca ttcgtccttg ttgtcgcgct tctggcggaa gcgggcgagg
     3001 ccgccgtcgc cgccggtggc gacgccgcgc tggtagccct ccatgtcctg ggtgacgacg
     3061 agcttgtcac tgcggatggt gagggtgccc tgggtgagga tgacgtgccc ttcgaagatg
     3121 tggatcttgt tcttgtcgtc cacggtgacc ttgtcggctt cgatgttgac gggcttggag
     3181 cggtcggcct tttcggcgtg ggcgaagggc gtcgcgatca gcgcggcgag ggcgaggagg
     3241 aggggccgga tgcgggaagt catggtttgg aggcctgctt gcggtagaag gtgccccgga
     3301 cctgaccgtg gaggtgcagt tcgccggcga tgttgtcggc gtccatgcgt tcggcggtga
     3361 cgacggtctg accctgggtc agggtgacgg gagtatcggt gagcatcttc tcgtcgtcgg
     3421 gccagacggt catccggtcg gagcgcagcg tggattcggg cttgtccggt gtggcggcgc
     3481 ggcgggcgaa gacgttgccg gacatgagca ccttctcgcc ttccttgctc accagggcgc
     3541 ggtcggactc gagatgcatg ggctcgggcc ggttgaggta gtcgaggcgg gggcgggtga
     3601 ggtccgagga ttcgtcgtcg gcgaagtggg tcatcttgtc ggcgacgagg acgtactggg
     3661 tcttgccggt gacgtcgtaa cggcgcagct tgacctgctc gacgatgaag tcgggctcgt
     3721 ggcggctgtt gtcctggggc agggtctgat cctgctgggt gatacggtcg agccagatgc
     3781 tggcgccggc cagcaggccc agcacgatgg cgggaaagag actgtggacg cggtctttca
     3841 tgcgggcgcg gacggggcgg ggcggcgcat cgtcagatga ccttggcctg catcaggatg
     3901 atgtggttga gggcgccggt aagctggcgg gcgtcgtcca ccaccagaag ctggctgatg
     3961 cgcaggcgct ccatcatctc ggcggcctcg acggcgaggg cttcggcgcg gatggtgcgc
     4021 gggttgcggg tcatgacgtc ggccacgcgg gcgctggcaa agtcgcagcc gccggcgagg
     4081 gaacggcgca ggtcgccgtc ggtgtagatg ccgacgacct gatcggcgcc gtccacgatg
     4141 gcggtcatgc cgaggccgcc gcgggtcatt tcgaggagcg cgtcgccgag ggtggcgtct
     4201 tcggtcaccc tgggcaggcc ggggccggag gtcatgatgt cggagacgcg ggtgagcagg
     4261 cgccggccga gggtgccgcc gggatgggag cgggcgaagt cttccgggcc gaagccgcgg
     4321 gcgtcgagga gggccaccgc gagggcatcg ccgagggcga gggaggcggt ggtgctggcg
     4381 gtgggcgcca ggttgtgcgg gcaggcctct tccttgacgg cgccgtcgag atgcacgtcg
     4441 gcctcgcggg cgagggggga atcggggttg ccggtgaggg cgatgaggcg ggcaccggag
     4501 cgcttgacca gggggacgat gcgcaggagc tcgtcgccgg cgccggaata ggagacggcg
     4561 atgaggacgt cgtccggggt gatcatgccc aggtcgccgt gggcggcttc ggcggcgtgc
     4621 acgaagtagg ccggcgtgcc ggtgctggcg agcgtggcgg cgagcttgcg gccgatgtg
//
LOCUS       DAOQPA010000189         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_11796_length_4630_cov_19.526120, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000189 DAOQPA010000000
VERSION     DAOQPA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11796_length_4630_cov_19.526120"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="ffh"
                     /locus_tag="PKX01_16445"
     CDS             complement(<1..1334)
                     /gene="ffh"
                     /locus_tag="PKX01_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMZ77776.1"
                     /translation="MLDNLTTRLAKVVKTLRGQARLTEDNIQDALREVRMALLEADVA
                     LPVVKDFIARVKEKAVGTEVISSLTPGQALVGVVHRELTELMGGAHTGLNLATQPPAI
                     ILMAGLQGAGKTTTTGKLGKLLVERMKKKVLAVSTDVYRPAAIAQLQTVAQQAGVEFF
                     PSTPDQKPVDIALAAVDYARRHYMDVLLVDTAGRLAIDQAMMDEIKALHAAVNPVETL
                     FVVDAMLGQDAVNTARAFNEALPLTGVVLTKLDGDSRGGAALSVRHVTGKPLKFAGVG
                     EKLAGLEEFHPERMASRILGMGDILSLVEDARRGVDEEKAKQFAQKLKSGKGFDLNDF
                     KDQIGQMRKMGGLSSMLDKLPAQFAQAAGQLQGGVEEKAIRRIEGIINAMTPLERTKP
                     ELIKASRKRRIATGAGVTVQEVNRLLSQFEQTQKMMKQFSKGGMQKMMRAMKG"
     gene            1373..2200
                     /gene="ccsA"
                     /locus_tag="PKX01_16450"
     CDS             1373..2200
                     /gene="ccsA"
                     /locus_tag="PKX01_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ77777.1"
                     /translation="MPVILLHLLPASLYALLGLHFWRSRWLQPALGPRQELLPWERGA
                     LLAALGAHGYALHHAFFASNGMVFGFSLALSLMLWLAVLFYWIESLYARLDGLQALAM
                     PAAALCALLPAFFPEQHVLENAHSPLFRAHFVVAMLAYSLFTLAALHALLMSVAERRL
                     HGARLSRAFASLPPLLTMEALLFRLIGIAFVLLTLTVGSGVLFSEVLFGRAFRFDHKT
                     VFAILSWLIFGGLLIGRHTRGWRGRKALRWTLAGFVALMLAYVGSRFVAEVLLGRAA"
     gene            2254..3528
                     /locus_tag="PKX01_16455"
     CDS             2254..3528
                     /locus_tag="PKX01_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HMZ77778.1"
                     /translation="MDSIPLSVQSAILAALLALSGFFSMAETAMMAANRYRLRTLAKQ
                     GSRGARLALDLLGQTDKLLGIILLFNNLINAASATLVSVITIQLFGNEEWALGVSTLG
                     VTFLILVFSEITPKVIGANHADHMACVVSYPLSILLRGLYFVIWFVNLFVAGLLRLFR
                     LQSNGEGENQPMSTEELRTIVLEAGSFIPPKHRSILVNLFELEDITVEDVMIPRHQIE
                     ALDLSQPRDVILHQIATCYHTRIPVFDAHMDAVAGVLHVRRILAQSLEHPVDAADIRG
                     MLSPPYFIPASTPIYSQLQFFQENRERIGLVVDEYGELLGLVTLEDIIEELIGKFTTS
                     APDASDKLAWNKDGSVIADGSQTIRTLNRKLGLALPVDGPKTLNGLLLEHLQDIPESG
                     VSVRIAGVPIEVIQAEDRRIKNARLFRPRSAG"
     gene            3618..>4630
                     /locus_tag="PKX01_16460"
     CDS             3618..>4630
                     /locus_tag="PKX01_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HMZ77779.1"
                     /translation="MSASPQVALSGLARALVQHGRLMESEALSCTTPSGNPNEFIIEA
                     IKRGLISAAEVARFSAATFGYPLLDLGSFDEAHFALDAIDRKLMAKHQAVPLGRRGNR
                     LTLAMADPSNLRVLDEIRFQSGLAVDPVVVEAPKLVELVERLSESASTVLKDMTGDEF
                     DMDLLGKEGASEAQIEDVNSEVDDAPVVRFIQKLLIDAINEGASDVHFEPYEKYYRIR
                     FRTDGILREIAQPPLVLKEKIAARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRV
                     STLPTLHGEKIVMRILDPTSAMLGIDALGYDPDQREALLAAVERPYGMVLVTGPTGSG
                     KTVS"
BASE COUNT          724 a         1651 c         1375 g          880 t
ORIGIN      
        1 cccttcatgg cgcgcatcat cttctgcatg ccgcccttgg agaactgctt catcatcttc
       61 tgggtctgct cgaactggct gaggaggcgg ttgacctcct gcacggtcac cccggcaccg
      121 gtggcgatgc ggcgcttgcg ggaggccttg atcagctcgg gcttggtccg ctccagcggc
      181 gtcatggcgt tgatgatgcc ctcgatgcgg cggatcgcct tttcctccac gccgccctgc
      241 aattggcccg cggcttgggc gaactgggcg gggagcttgt cgagcatgct gctgaggccg
      301 cccatcttgc gcatctggcc gatctggtcc ttgaaatcgt tgagatcgaa acccttgccg
      361 ctcttgagct tctgggcaaa ctgcttggcc ttctcctcgt ccacgccacg gcgggcgtcc
      421 tccaccaggg acaggatgtc gcccatgccc aggatgcggc tggccatccg ctcggggtgg
      481 aattcctcca ggccggcgag cttctcgccc acgcctgcga acttgagggg ctttccggtg
      541 acatgccgta ccgacagcgc cgcgccgccg cgggaatcgc cgtccagctt ggtgagcacc
      601 acgcccgtga ggggcagcgc ctcgttgaag gcgcgggcag tgttgaccgc gtcctgaccc
      661 agcatcgcgt ccaccacgaa gagggtctcg acgggattga ccgcagcgtg cagcgccttg
      721 atctcgtcca tcatcgcctg gtcgatggca agccgcccgg cggtatccac cagcagcacg
      781 tccatgtagt gccggcgggc gtaatccacc gctgcgaggg cgatgtccac gggcttctgg
      841 tccggcgtgg acgggaagaa ctcgacgccg gcctgctggg cgacggtctg caactgggcg
      901 atggccgcag gacgatagac gtcggtggac accgccagca ccttcttctt catccgctcc
      961 acgagcagct tgccgagctt gccggtggtg gtggtcttgc ccgccccctg cagacctgcc
     1021 atgaggatga tggccggcgg ctgggtcgcc aggttgagcc ccgtatgggc cccccccatc
     1081 agctcggtga gctcccggtg caccacgcca acaagggcct ggcccggcgt gagggaggag
     1141 atgacctcgg tgccgacggc cttttccttg acccgggcga tgaaatcctt gacgaccggc
     1201 agggcgacgt cggcttccag cagggccatg cgcacctcgc gcagggcatc ctggatgttg
     1261 tcttcggtga ggcgggcctg accccggaga gtcttgacga ctttggccag gcgcgtggtg
     1321 aggttgtcca gcatgatggt tcgtgaccgg ggccttggag taaacttgaa cgatgccggt
     1381 cattctactg catctcctcc ccgcctccct gtacgccctg ctgggcctgc atttctggcg
     1441 atcccgctgg cttcagcctg ccctcggccc ccgtcaggaa cttcttccct gggaacgggg
     1501 cgcgctgctg gccgccctcg gcgctcacgg ctacgcgctg catcatgcct tcttcgcctc
     1561 caatggcatg gtcttcggtt tcagtctggc cctgtccctg atgctatggc tggcggtgct
     1621 cttttactgg atcgagagcc tctacgcccg cctcgacggc ctgcaggccc tggccatgcc
     1681 ggctgcggcc ctgtgcgcgc tgctgccggc cttctttccg gaacagcacg ttctggagaa
     1741 cgcccactcg cccctgttcc gcgctcactt cgtggtggcc atgctggcct acagcctatt
     1801 caccctggct gcgctccatg ccctgctcat gtcggtggcc gaacggcgcc tgcacggcgc
     1861 ccgcctgagc cgcgccttcg cctccctgcc gccgctgctg accatggagg ccctgctgtt
     1921 ccggctgatc ggcatcgcct tcgtgctgct caccctcact gtggggtccg gcgtcctgtt
     1981 ctcggaagtg ctcttcggac gtgccttccg cttcgatcac aagaccgtct tcgcgatcct
     2041 gtcgtggctg atcttcggcg gactcctgat cggtcgccac acccgcggct ggcgcggtcg
     2101 caaggccctg cgctggacgc tggcgggctt cgtggccctg atgctggcct acgtgggcag
     2161 ccgatttgtc gccgaggtgc tgctcgggcg cgccgcttga caaggcccca tccgggctga
     2221 atactcggcc ggtcgtcatc tccccgtcct cgcttggaca gcatccccct ctccgtccag
     2281 tccgccatcc tggcagccct gctggcgcta tccggttttt tctcgatggc cgaaacggcc
     2341 atgatggcgg ccaaccgcta ccgcctgcgc acgctggcca agcagggttc ccgcggcgcc
     2401 cgcctcgccc ttgatctgct cggccagacc gacaagctgc tgggcatcat cctgctgttc
     2461 aacaacctca tcaacgccgc ctcggccacg ctggtgagcg tgatcaccat ccagctcttc
     2521 ggtaacgagg aatgggccct gggtgtgagc accctgggcg tcaccttcct gatcctcgtg
     2581 ttctcggaga tcacgcccaa ggtcattggt gccaaccacg ccgatcacat ggcgtgcgtc
     2641 gtgagctatc ccctgtcgat cctgctgcgc gggctgtatt tcgtgatctg gttcgtgaac
     2701 ctgttcgttg ccggcctgct caggctgttc cgcctgcaat cgaacggcga gggcgagaat
     2761 cagcccatgt ccaccgagga attgcgcacc atcgtgctgg aagccggctc cttcatcccg
     2821 cccaagcacc gcagcatcct ggtcaatctg ttcgaactgg aggacatcac cgtcgaggac
     2881 gtgatgattc cccgccatca gatcgaggcc ctggacctct cccagccccg cgacgtgatc
     2941 ctgcaccaga tcgccacctg ctatcacacg cgcatcccgg tattcgacgc gcacatggac
     3001 gcagtcgcgg gcgtcctcca tgtcaggcgc atcctcgccc aatccctcga acacccggtg
     3061 gatgcggcgg acatccgcgg catgctgtcg cccccgtatt tcattccggc cagcacgccg
     3121 atctactccc agctccagtt cttccaggaa aaccgcgaac gcatcggcct ggtggtggac
     3181 gagtacggcg aactgctggg tctcgtgacc ctcgaagaca tcatcgagga actcatcggc
     3241 aaattcacca ccagcgcgcc ggacgcctcc gacaaactgg cctggaacaa ggacggcagt
     3301 gtcatcgccg atggcagcca gacgatccgc accctcaatc gcaagctcgg cctggccctg
     3361 ccggtggacg ggcccaagac cctcaatggc ctgcttctgg aacatctcca ggacattcct
     3421 gaatccggtg tctccgtgcg cattgccggc gtccccatcg aagtcatcca ggccgaagac
     3481 cggcgcatca agaacgcccg cctgttccgc ccgcgcagcg ccggataaaa acactgtgag
     3541 tgcggcttta caagcccacc gccgggcggc atgatccgcc caattccacc cacaccccct
     3601 cacgccccca gcccggcatg tccgcgtcgc ctcaagtcgc cctgagcggg ctcgcccgcg
     3661 ccctcgtcca gcacggtcgc ctcatggaat ccgaggccct gtcctgcacg acgccctccg
     3721 gcaatcccaa cgaattcatc atcgaggcca tcaagcgtgg actgatcagc gccgccgagg
     3781 tcgcccgctt cagcgccgcc acctttggtt atccgctgct ggatctgggc agcttcgatg
     3841 aagctcattt cgccctcgac gccatcgacc gcaagctgat ggccaagcac caggccgttc
     3901 ccctggggcg ccgcggcaac cgcctcaccc tcgcgatggc cgatccgtcg aacctgcggg
     3961 tgctcgacga aatccgcttc cagagcggtc tcgccgtgga cccggtggtc gtcgaggcgc
     4021 ccaagctcgt cgagttggtc gagcgcctgt ccgaatccgc gtccaccgtc ctcaaggaca
     4081 tgacgggcga cgagttcgac atggatctgc tgggcaagga aggcgcctcg gaagcccaga
     4141 tcgaggacgt gaactcagag gtagacgacg ccccggtggt gcgcttcatc cagaagctgc
     4201 tcatcgacgc catcaacgaa ggcgcctccg acgtccactt cgaaccctac gagaagtact
     4261 accgcatccg tttccgcacc gacggcatcc tgcgggagat cgcccagccg ccgttggtgc
     4321 tcaaggaaaa gatcgccgcg cggatcaagg tcatctcgcg cctggacatc tccgaaaagc
     4381 gggtgcccca ggacggtcga atgaagctcg tcctgtccaa gaacaaggcc atcgacttcc
     4441 gcgtctcgac cctgccgacc ctgcacggcg aaaagatcgt gatgcgcatc ctcgatccca
     4501 cgtcagccat gctgggcatc gacgccctcg gctacgatcc tgatcagcgc gaagccctgc
     4561 tcgccgccgt cgagcgcccc tacggcatgg tgctcgtcac cggccccacc ggcagcggca
     4621 agacggtgtc
//
LOCUS       DAOQPA010000190         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_12266_length_4521_cov_16.083296, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000190 DAOQPA010000000
VERSION     DAOQPA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12266_length_4521_cov_16.083296"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1748)
                     /locus_tag="PKX01_16465"
     CDS             complement(69..1748)
                     /locus_tag="PKX01_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ77780.1"
                     /translation="MSERDAEPAGPALSWAQELVEAAPDPIIVIDQGGAIVFANGQTE
                     RLFGYPRNELLGQPIEHLVPERFRHTHHAKRASFQAHPSFRPMGSGLDLTARCRDGRE
                     IPVEISLAPLHTADGVFISAAIRDITERRRTQKALRDARDQLEARVHARTAELEHANQ
                     ALQTEMADRLKAEGALRQAQKMEAVGQLTGGIAHDFNNLLTVIMGNLQMLLRQTRDNA
                     LASELAQAAFKAARRGSELNRTLLAFSRRQHLAPAAVDLNELVTHMGTMLQRTLGEQI
                     NIQLGLAPGLPRALADPGQLETAVLNLAVNARDAMPDGGTLSLDTASVVLDSHYTATE
                     VDITPGRYLRLTVSDSGHGMDAATVARACEPFFTTKEVGQGSGLGLAMVYGFVKQSGG
                     HIKIYSEPGLGTSIKLFLPEVQRDADTPTDAPDSPTDVEPRGGHETLLIVEDEEAVRN
                     MVCRVLESLGYRILVATQGNEALELLQTHPEIALLFTDVVLPGGLRGPDIARHARSLR
                     PDLPVLFTSGYTGTALPLQDSTDTPPFPLITKPYPIEELARMIRGALEGGG"
     gene            complement(1745..2254)
                     /locus_tag="PKX01_16470"
     CDS             complement(1745..2254)
                     /locus_tag="PKX01_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ77781.1"
                     /translation="MIDDTHGRVLYARDGDTWVLRFTGAIRYTMAHAVDSFLDELFSR
                     QHPGRICVDLNDTESIDSTGIGLLAKLANGLARHGGERPVFFTSNPEILETLHNVCLD
                     DLCTLVAGAPTAVAANEIPATTPDERQLAGTIVAVHRLLCDMNAENRAKFQSVVDAFE
                     RDLAPEAHK"
     gene            complement(2247..3431)
                     /locus_tag="PKX01_16475"
     CDS             complement(2247..3431)
                     /locus_tag="PKX01_16475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ77782.1"
                     /translation="MPPTEILIIDDDPAVRDMLSLYLRSDGHTVRTAASGPEGIARCH
                     EHPPSLVLCDLSMPGMSGLAVMSRLTADFPDLPVLVVSGTGDLGDAIEALKLGAWDFV
                     TKPIEDLAVLDHAIGRAFERARLLAENRAYRIHLENTNARLETTLRRLEEDEEGGRRI
                     QFALLPDARVDFRDITCTHFLVTSAILGGDFVDYFRIDDARFGFYMADVSGHGVPSAV
                     VTVLLKGFVSRFLENHRHYGDPTLLDPAALLAALNPLVLDGKHGKYLTMFYAVVDLDA
                     NTLSFANGGHFPLPLISDGGEVRELGGRSRPVGLFRDAQFNTETLTLPDRFALRLYSD
                     GVLELLDGPGNDARRATLRSVSRSLDLDARNLAARLGMESNRPLPDDASILSIRRHAP
                     HD"
     gene            complement(3576..>4521)
                     /locus_tag="PKX01_16480"
     CDS             complement(3576..>4521)
                     /locus_tag="PKX01_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HMZ77783.1"
                     /translation="GMGIDKPDVRFVAHLDLPRSIEGYYQETGRAGRDGLPAEAWMTY
                     SIADLVQQRRFIDQSEAGDDIKRVATAKLDALLGLCETTGCRRQRLLGYFDEDSDPCG
                     NCDTCLSPPQVRDATESARKALSCVFRTGSRFGAGHVVDVLLGKTTDKVREWQHDQVS
                     TFGIGTEFDDKGWRTLIRQLVAHGALAVDHERYGALTLTEAARPLLRGETGFTLRLDT
                     VAPKGRKSAPSARRAADWPEATGAEARLLDRLRSWRAATAKARNVPAYVIFHDATLRD
                     IAHQRPDSREALAAISGVGDRKLDAYADDLLGLLEDAS"
BASE COUNT          591 a         1185 c         1980 g          765 t
ORIGIN      
        1 gtcggtggcg aagctggccg ggtcttgggc gtatgcctcg caagcgcggg tgacgctgcg
       61 gtcgaagtct accccccacc ctccagcgcc ccccggatca tccgtgccag ttcctcgatg
      121 ggatagggct tggtgatcag cgggaagggg ggcgtgtcgg tgctgtcctg cagggggagg
      181 gcggtgccgg tgtagccgga ggtgaagagg acgggcaggt cggggcgcag gctgcgggcg
      241 tggcgggcga tgtcggggcc gcggaggcca ccggggagga cgacgtcggt gaagaggagg
      301 gcgatctccg ggtgggtctg gaggagttcg agggcttcgt tgccctgggt ggcgacgagg
      361 atgcggtagc cgagggattc gaggacgcgg cagaccatgt tgcggacggc ttcttcgtct
      421 tcgacgatga gcagggtctc gtggccgccg cggggttcga cgtccgtcgg gctgtcgggg
      481 gcgtcggtgg gggtgtcggc gtcgcgctgc acttcgggca ggaagagctt gatggaggtg
      541 ccgaggccgg gctcgctgta gatcttgatg tggccgccgg attgtttgac gaagccatac
      601 accatggcga ggccgaggcc gctgccctgg ccgacttcct tggtggtgaa gaagggttcg
      661 caggcgcggg cgacggtggc ggcgtccatg ccgtggccgg agtcggagac ggtgaggcgc
      721 aggtagcggc cgggggtgat gtctacttcg gtggcggtgt agtggctgtc gaggacgacg
      781 ctggcggtgt cgaggctgag ggtgccgccg tcgggcatgg cgtcgcgggc gttgacggcg
      841 aggttgagca cggcggtctc gagctggccg gggtcggcga gggcgcgggg cagcccgggg
      901 gcgaggccga gctggatgtt gatctgctcg cccagagtgc gctggagcat ggtgcccatg
      961 tgggtgacga gctcgttgag gtcgacggcg gcgggggcga ggtgctggcg ccgggagaag
     1021 gcgagcaggg tgcggttgag ctcgctgccg cggcgggcgg ccttgaaggc ggcctgggcg
     1081 agttcggagg cgagggcgtt gtcacgggtc tggcgcagga gcatctgcag gttgcccatg
     1141 atgacggtga ggaggttgtt gaagtcgtgg gcgatgccac cggtgagctg gccgacggct
     1201 tccatcttct gggcctgacg gagggcgcct tcggccttga gccggtcggc catctcggtc
     1261 tgcagggcct ggttggcgtg ttcgagttcg gcggtgcggg cgtgtacgcg ggcttcgagc
     1321 tggtcgcggg cgtcgcggag ggctttctgg gtgcggcgcc gttcggtgat gtcgcggatg
     1381 gcggcagaga tgaagacgcc gtcggcggtg tgcagggggg cgaggctgat ctcgacgggg
     1441 atctcgcgac cgtcgcggca gcgcgcggtg aggtcgagcc cgctgcccat ggggcggaag
     1501 ctggggtggg cctggaagct ggcgcgcttg gcgtggtggg tgtggcggaa gcgttcgggg
     1561 acgaggtgtt cgatgggctg gccgaggagt tcattgcggg gatagccgaa gagccgctcg
     1621 gtctgaccgt tggcgaagac gatggcgccg ccctggtcga tgacgatgat ggggtcgggg
     1681 gcggcttcga cgagttcctg ggcccaggac agggcggggc ccgcgggctc ggcgtcgcgt
     1741 tcgctcattt gtgggcttcc ggggcgaggt cccgttcgaa ggcgtccacg acgctctgga
     1801 acttggcgcg gttctcggcg ttcatgtcgc acagcagacg atggacggcg acgatggtgc
     1861 cggccagctg ccgttcgtcc ggcgtggtgg cggggatctc gttggcggcg acggcggtgg
     1921 gagcgccggc gacgagggtg cacaggtcgt ccaggcagac gttgtgcagg gtctctagga
     1981 tctcggggtt ggacgtgaag aagaccggac gttcgccgcc gtggcgggcg aggccattgg
     2041 cgagcttggc gaggaggccg atgccggtgc tgtcgatgga ttcggtgtcg ttgaggtcga
     2101 cgcagatgcg gccggggtgc tgacggctga agagctcgtc gaggaagctg tcgacggcgt
     2161 gggccatggt gtagcggatg gcgccggtga agcgcaggac ccaggtgtcg ccgtcgcgcg
     2221 cgtagagcac gcggccgtga gtgtcgtcaa tcatgggggg cgtgcctcct gatgctgagg
     2281 atgctggcgt cgtccgggag gggacggttg ctttccatgc ccagacgggc cgcgaggttg
     2341 cgggcgtcga ggtcgaggga ccgggagacg ctgcgcaggg tggcgcgccg tgcgtcgttg
     2401 ccggggccgt cgagcagttc gagcacgccg tcggagtaga ggcggagggc gaaccggtcg
     2461 ggcagggtga gggtttcggt gttgaactgg gcgtcgcgga agaggccgac cggacggctg
     2521 cggccgccga gctcgcgcac ctcgccgccg tcgctgatga ggggcagcgg gaagtggccg
     2581 ccgttggcga agctgagggt gttggcgtcg aggtcgacga cggcatagaa catggtgagg
     2641 tacttgccat gcttgccgtc gaggacgagg gggttgaggg ccgccaggag ggccgcgggg
     2701 tcgagcaggg tggggtcgcc gtagtggcgg tggttctcga ggaagcggct gacgaagccc
     2761 ttgagcagca cggtgacgac ggcggagggg acgccgtggc cggagacgtc ggccatgtag
     2821 aagccgaagc gggcgtcgtc gatgcggaag taatcgacga agtcaccccc gaggatggcg
     2881 gaggtgacga ggaaatgggt gcaggtgatg tcgcggaagt ccacgcgggc atcgggcagc
     2941 agggcgaact ggatgcggcg gccgccttcc tcgtcttctt cgaggcgccg cagggtggtt
     3001 tcgaggcggg cgttggtgtt ttccaggtgg atgcggtagg cccggttctc ggcgagcagg
     3061 cgggcccgtt caaaggcgcg gccgatggcg tggtcgagca cggccaggtc ttcgatgggc
     3121 ttggtgacga aatcccaggc gccgagcttg agcgcttcga tggcgtcgcc caggtcgccg
     3181 gtgccggaga cgacgaggac cgggagatcg gggaaatcgg cggtgagccg ggacatgacg
     3241 gcgaggccgc tcatgccggg catgctcagg tcgcacagga cgagggacgg cgggtgctca
     3301 tgacagcggg cgatgccctc ggggccgctc gcggcggtgc gcacggtgtg cccgtcgctg
     3361 cgcaggtaca ggctgagcat gtcccggacg gcgggatcat cgtcgatgat caggatttcc
     3421 gttgggggca acgggtctcc ccttttttgt ggtgaggggt cgatggatcc gtgggtcaga
     3481 cggtccgttg gggtcacgaa agtctctcat gctctgaccc tgccgtatag gccaaagttt
     3541 cgacgcagcc cctgcgcgcc gttgggcgcg gcgcgtcagg aggcgtcttc gaggaggccc
     3601 aggaggtcgt cggcgtaggc gtcgagtttg cggtcgccga cgccggagat ggcggcgagg
     3661 gcttcgcggc tgtccgggcg ctggtgggcg atgtcgcgca gggtggcgtc gtggaagatg
     3721 acgtaggcgg gcacgttgcg ggccttggcg gtggcggcgc gccagctgcg caggcggtcg
     3781 aggaggcggg cttcggcgcc ggtggcttcg ggccagtcgg cggcgcggcg ggcactgggg
     3841 gcgctcttgc ggcccttggg ggcgacggtg tcgaggcgca gggtgaagcc ggtttcgccg
     3901 cgcagcagcg ggcgggcggc ttcggtgagg gtgagggcgc cgtagcgttc gtggtcgacg
     3961 gcgagggcgc cgtgggcgac gagctggcgg atgagggtgc gccagccctt gtcgtcgaac
     4021 tcggtgccga tgccgaaggt gctgacctgg tcgtgctgcc actcgcgcac cttgtcggtc
     4081 gtcttgccga ggagcacgtc caccacgtgg ccggcgccga agcggctgcc ggtgcggaag
     4141 acgcaggaga gggccttgcg ggcggactcg gtggcgtcgc gcacttgcgg ggggctgagg
     4201 caggtgtcgc agttgccgca ggggtcggag tcttcgtcga agtagccgag gaggcgctgg
     4261 cggcggcagc cggtggtctc gcagaggccg aggagggcgt cgagcttggc ggtggcgacg
     4321 cgcttgatgt cgtcgccggc ttcggactgg tcgatgaagc ggcgctgctg gacgaggtcg
     4381 gcgatgctgt aggtcatcca ggcttcggcg ggcaggccgt cgcggccggc gcggccggtt
     4441 tcctggtagt agccttcgat ggagcggggc aggtcgaggt gggcgacgaa gcgcacgtcg
     4501 ggcttgtcga tgcccatgcc g
//
LOCUS       DAOQPA010000191         4480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_12454_length_4480_cov_20.512316, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000191 DAOQPA010000000
VERSION     DAOQPA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12454_length_4480_cov_20.512316"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKX01_16485"
     CDS             <1..755
                     /locus_tag="PKX01_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HMZ77784.1"
                     /translation="PYGMVLVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINL
                     PGINQVNVNEKAGLTFASALRAFLRQDPDVIMVGEIRDLETAEISIKAAQTGHLVLST
                     LHTNDAPSTLERLRNMGVAPFNIASSVILITAQRLARRLCSCKQPMDIPIEALLTAGF
                     TEQDLDGTWQPYGPKGCERCKGSGYKGRVGIYQVMPISEEIARIIMTNGNSMDIAAQA
                     QLEGVKDLRQSGLLKVKQGVTSLTEVLACTNE"
     gene            778..2022
                     /locus_tag="PKX01_16490"
     CDS             778..2022
                     /locus_tag="PKX01_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMZ77785.1"
                     /translation="MATVPKKTSSRAPVTAKEFLFVWEGKDKTGKIIRGEMRATTPNV
                     VQTVLRRQGILAHKITKQAFARGKKITDKDIALFTRQLSTMMRSGVPLLQSFDIAIKG
                     CGNPSFARLLNEVRSDVETGSSLSQAFRRHPLYFDALFCNLVAAGEQAGILDSLLDRL
                     ATYKEKMLAIKSKIKSAMFYPIAVVVVAALVIAVMMLFVVPEFKKVFTSFGADLPAPT
                     MVVIAISDFFVEYWYLFFGILAGTVFGISFTYKRSQAMQIAVDRAILQIPVIGEVVRK
                     ATIARWTRTLSTMFAAGVPLVEALDSVGGAAGNIVYKTATKQIQSDVSTGTSLTVSMQ
                     NSLVFPTMVVQMVSIGEESGQLDSMLGKVAEFFEREVDDAVAGLSQLLEPIIMVFLGV
                     VIGGLVVAMYLPIFKLGAVVGG"
     gene            2044..2892
                     /locus_tag="PKX01_16495"
     CDS             2044..2892
                     /locus_tag="PKX01_16495"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HMZ77786.1"
                     /translation="MQIFTEPPVFTAVCVLLGLFVGSFLNVVIHRLPIMMEREWQAEA
                     AALRGEAGEGADPFNLATPRSRCPHCGHLISALENIPVVSYLLLRGRCRHCSAPISLR
                     YPLVEVICAMLSGITAWHFGFGAAGLGALAFVWAMIALTFIDLDTQLLPDSITLPLLW
                     LGLLLNLQQAFATLPDAVVGAAAGYLTLWSVYWLFKLTTGKEGMGYGDFKLLAAIGAW
                     FGWQILPLTILLSSVVGALVGVGLIVFRRHGREVPIPFGPYLAAAGLLALYWGRPLTD
                     AWLGGF"
     gene            3038..3301
                     /locus_tag="PKX01_16500"
     CDS             3038..3301
                     /locus_tag="PKX01_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMZ77787.1"
                     /translation="MPIYAYRCNACGFQKDHLQKLSDAPLTTCPSCGADSYAKQLTAP
                     GFQLKGNGWYATDFKGGSSSASKAEPAKADAPATPCGGSCACH"
     gene            3327..3968
                     /locus_tag="PKX01_16505"
     CDS             3327..3968
                     /locus_tag="PKX01_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HMZ77788.1"
                     /translation="MKKYFVTGLLIWIPLVITAVVLAWIVNTLDQILLVVPPSVRPEA
                     FIGFHVPGIGVVVSLLLILLTGLAAANFVGQHLVKFWEKLLSRIPVVKSIYYSVKQVS
                     DTLLSSNGQAFRKALLIQYPREGMWTIGFQTGIPGGDAAHHLGSDFVSVYVPTTPNPT
                     SGFFLMLPRRDAIELDMSVDEALKYIISMGVVAPPPHRAPAPSHAATPALQNP"
     gene            4023..>4480
                     /locus_tag="PKX01_16510"
     CDS             4023..>4480
                     /locus_tag="PKX01_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMZ77789.1"
                     /translation="MRTQYCGLVSAAYLDQIVTLCGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRPEMFQVAESVRNEFVLKITGKVRRRPAGTENSQLVSGEIEVLCHEIEVL
                     NVSATPPFQLDEENLSETTRLTHRVIDLRRPQMQKNMILRYRTAMAFRRFL"
BASE COUNT          781 a         1555 c         1280 g          864 t
ORIGIN      
        1 gcccctacgg catggtgctc gtcaccggcc ccaccggcag cggcaagacg gtgtctcttt
       61 acacctgcct gaacatcctc aacaaggccg gcgtgaacat ctccaccgcc gaagacccgg
      121 cggaaatcaa cctccccggg atcaaccagg tcaacgtgaa cgaaaaggcc ggcctcacct
      181 tcgcgtcggc cctgcgcgcc ttcctccggc aggatccgga tgtcatcatg gtgggcgaaa
      241 tccgcgatct ggaaaccgcc gaaatttcca tcaaggccgc ccagaccggc cacctggtgc
      301 tttccacgct gcacaccaac gatgcgccct ccacgctgga acgcttaaga aacatgggcg
      361 tcgcgccgtt taacattgcg tcgtcagtga tcctgatcac cgcccagcgc ctggcccggc
      421 gtctgtgctc gtgcaagcag ccgatggaca ttccgatcga agccctgctc accgcgggat
      481 tcaccgaaca ggatctggac ggcacctggc aaccctatgg gcccaagggg tgcgagcggt
      541 gcaagggctc cggctacaag ggacgggtcg gcatttacca ggtgatgccg atctcggaag
      601 agatcgcacg tataatcatg acgaacggca actccatgga catcgcggcc caggcccagc
      661 tcgagggcgt gaaagatctg cgccagtcgg ggttgctcaa ggtcaagcag ggcgtcacct
      721 ccctgaccga agtcctcgcc tgcaccaacg agtgaccgcc tcccgcgaaa gtttttgatg
      781 gccaccgtac ccaaaaagac gtcctcccgc gctcccgtca ccgccaagga attcctcttc
      841 gtctgggaag gcaaggacaa gaccggcaag atcatccgcg gagaaatgcg cgcgaccact
      901 cccaatgtgg tccagacggt attgcgccgc caggggatcc tggcccacaa gatcaccaag
      961 caggccttcg cccgtggcaa gaagatcacc gacaaggaca tcgccctgtt cacccggcaa
     1021 ctgtccacaa tgatgcgctc cggcgttccc ctgctccagt ctttcgacat cgccatcaag
     1081 ggttgcggca atccgtcctt tgcccgcctt ctaaacgaag tccgctcgga cgttgaaact
     1141 ggcagcagtc tgtcccaggc attccgccga catcctctgt atttcgacgc cctgttctgc
     1201 aacctggtgg ctgctggcga acaggccggc atcctcgact cgctgctcga tcgcctcgcc
     1261 acctacaagg aaaagatgct ggcgatcaag tccaagatca agtcggccat gttctacccc
     1321 atcgcggtag tcgtggtagc tgcgctggtc atcgcggtga tgatgctgtt cgtggtgccc
     1381 gagttcaaga aggtcttcac cagtttcggc gcggatctgc ccgcgccaac aatggtcgtc
     1441 atcgccattt ccgacttttt cgtcgagtat tggtacctgt tcttcggcat cctcgcgggt
     1501 accgtcttcg gcatctcgtt cacttacaaa cgctcgcagg ccatgcagat cgccgtagac
     1561 cgggcgatcc tgcagattcc ggtgatcggc gaagtggtgc gaaaggccac catcgcacgc
     1621 tggacccgta ccctgtccac aatgttcgcc gccggcgtgc cgctcgtgga agccctcgac
     1681 tccgtcggcg gcgcggccgg caacatcgtc tacaagaccg ccaccaaaca gatccagtcc
     1741 gatgtcagca ccggtaccag cctcaccgtg tccatgcaga actccctggt gttccccacc
     1801 atggtggttc agatggtgtc catcggtgaa gaatccggcc aactcgactc catgctcggc
     1861 aaggtcgccg aattcttcga gcgggaagtg gacgatgccg tcgccggcct cagccagttg
     1921 ctcgaaccga taatcatggt cttcctcggt gtggtgatcg gcggtctcgt ggtggccatg
     1981 tacctgccca ttttcaagct gggtgccgtc gtgggcggct gacgcccggg cccgtctcga
     2041 tccatgcaga tctttaccga acctccggtc ttcacggccg tctgcgtgct gctcggactg
     2101 ttcgtcggca gcttcctcaa cgtcgtcatc caccgcctgc ccatcatgat ggaacgggag
     2161 tggcaggccg aagccgcggc cctgcgaggc gaagccggcg agggcgccga tccgttcaat
     2221 ctggccactc cgcgctcccg ctgcccccat tgcggccatc tgatttctgc gctcgagaac
     2281 atcccggtgg tcagctacct gctcctgcgg ggtcgctgcc ggcattgcag tgcgcccatc
     2341 agcctgaggt atccgttggt ggaagtgatc tgtgccatgc tgtccgggat caccgcctgg
     2401 catttcggtt tcggcgcagc cgggctgggc gccctggcct tcgtgtgggc catgatcgcc
     2461 ctgaccttca tcgatctgga cacgcagctc ctgcccgaca gcatcaccct cccgttgctg
     2521 tggctggggc tgttgctcaa ccttcagcaa gcatttgcaa cgctgcccga tgcagtggtc
     2581 ggcgcggcgg caggctatct caccctgtgg tcggtctatt ggctgttcaa gctcaccacc
     2641 ggcaaggaag gcatgggcta cggcgatttc aagctgctgg cggccatcgg cgcctggttc
     2701 gggtggcaga tccttcccct gacgattctc ctctcctccg tcgtcggtgc gctggtgggc
     2761 gtcgggctga tcgtgttccg gcgtcacggc cgggaggttc cgatcccctt cggcccctat
     2821 ctggcggccg ccggcctgct ggcgctgtac tggggccgcc cgctcacgga cgcctggctg
     2881 ggcggattct gacgcgcggc acgtctgccg atcaccccgt gcaggccccg cggggcttga
     2941 aacttcgccc ctcaccccca agtgcctgaa tgcgcgcctg tttcgcccga tgttgcgatg
     3001 cgctaaactt caccgaattg acttccggga gttcgtcatg cccatctacg cgtatcgctg
     3061 caacgcttgc ggctttcaga aagaccacct ccagaaactg agcgacgccc cgctcaccac
     3121 ctgcccgtcc tgtggcgccg acagctacgc caagcagctc acggcgcccg gcttccagct
     3181 taagggcaat ggctggtatg ccaccgactt caagggcggc agttcgtctg cctccaaggc
     3241 cgagccggcc aaggcggacg caccggccac gccctgtggc ggcagttgcg cctgtcactg
     3301 aaggttgtgg aaaccgacct gacccgatga aaaaatactt cgtcaccggc ctgctgatct
     3361 ggattccgct ggtcatcacc gccgtggtgc tggcctggat cgtcaatacc ctcgaccaga
     3421 tcctgctggt ggtgcccccc tcagtgcgcc cggaggcctt catcggcttt cacgtgccgg
     3481 ggatcggcgt cgtggtgtcc ctgctgctca tcctgctcac cggcctcgcg gcagccaact
     3541 tcgtcggcca acacctggtg aagttctggg aaaagctgct gtcgcgcatc ccggtggtga
     3601 aatccatcta ttacagcgtc aagcaggtct ccgacaccct gctttccagc aatggtcagg
     3661 cgttccgcaa ggcgctgctg atccaatatc cccgtgaagg gatgtggacc atcggcttcc
     3721 agaccggcat ccctgggggc gacgcagccc accatctggg cagcgatttc gtcagcgtct
     3781 atgtgcccac cacgcccaac cccacgtcgg gcttcttcct gatgctgccg cgccgcgacg
     3841 ccatcgaact cgacatgagc gtggatgagg ctctcaagta catcatctcc atgggcgtcg
     3901 ttgcgccgcc gccccaccgg gcgcccgcgc catcgcacgc ggccacgccg gccctgcaga
     3961 atccgtaagc cgcggctgcc ccgtcagccg cgttccgttt cctcaagaac ctagatccca
     4021 ccatgcgaac tcaatactgc ggccttgtct ccgccgccta cctcgaccag atcgtcaccc
     4081 tctgcggctg ggtccaccgt cgtcgcgacc acggcggcgt gatcttcatc gacctgcgcg
     4141 accgggaagg cctggtccag gtggtgtgcg atccggaccg cccggagatg ttccaggtgg
     4201 ccgagtcggt gcgcaacgag ttcgtcctca aaatcaccgg caaggtgcgc cgccggccgg
     4261 ccggcaccga gaacagccag ctcgtctccg gcgagatcga agtcctgtgt cacgagatcg
     4321 aagtcctcaa cgtctccgcc acgccgccct tccagctcga cgaggaaaac ctctccgaga
     4381 ccacccgcct cacccaccgg gtgatcgacc tgcgccgccc gcagatgcag aagaacatga
     4441 tcctgcgcta ccgcacggcc atggccttcc gccgcttcct
//
LOCUS       DAOQPA010000192         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_12578_length_4454_cov_19.791771, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000192 DAOQPA010000000
VERSION     DAOQPA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12578_length_4454_cov_19.791771"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..488)
                     /locus_tag="PKX01_16515"
     CDS             complement(105..488)
                     /locus_tag="PKX01_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HMZ77790.1"
                     /translation="MPWDDAQSLGVADMDATHREFVEHVDALASADDSAFPALFLSLQ
                     EHTRLHFEHEGKLMKACRFPAIGEHNSEHLRVLGEFAHFARGVNAGRLTPARAYIQAL
                     PGWFATHLATMDAALAGCLKRSRGE"
     gene            complement(488..1045)
                     /locus_tag="PKX01_16520"
     CDS             complement(488..1045)
                     /locus_tag="PKX01_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77791.1"
                     /translation="MLLDRYEQYFFNANFREAADEAGWEAYRGELVLKEGEIADDKGR
                     RKPPHEVLKQVVVLAKGDELKLISGHLDALQHIPGFVEKYAADLKADTIAVLFAVNID
                     DPFLAKIGEGTVAFIPLVQGMAWNELIDLVALEKGDFKGQSAAEKVVTVFNGFKGHKF
                     KYPETTLDDALKSTNNAKREMHGAV"
     gene            complement(1074..1910)
                     /locus_tag="PKX01_16525"
     CDS             complement(1074..1910)
                     /locus_tag="PKX01_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HMZ77792.1"
                     /translation="MTPELRATLLAGIQDGSIVPYLGAGVLADVKNVATGEPIPATSD
                     ALIIAMNGGKPMAPKLMYEFPRAAMNVELKRGRSTVNKFLTRTYGETQWTRGAVHDWI
                     KAIRPRYVIDINRDTQLQDSYADVPHNLIVGIARIGGTTLRYKIFFWDGSSYQRAEEL
                     KLEHPILFKPVGTPRPEPTYIASDADYVDFITELMGGFSIPPQLKEFRKGKQYLLAGL
                     RLNRDTERMVMADIIYSAAEPAGWVFIAEPTDKERRYCKKIGLEIVDADIFDLIGEPQ
                     PA"
     gene            2691..3572
                     /gene="nifH"
                     /locus_tag="PKX01_16530"
     CDS             2691..3572
                     /gene="nifH"
                     /locus_tag="PKX01_16530"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HMZ77793.1"
                     /translation="MAKLRQCAIYGKGGIGKSTTTQNLVAALAEAGKKVMIVGCDPKA
                     DSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSVGFGGVKCVESGGPEPGVGCAGR
                     GVITAINFLEEEGAYDEDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAM
                     YAANNISKGIVKYANSGGVRLGGLICNSRNTDREDELIIALAARLGTQMIHFVPRDNA
                     VQHAEIRRMTVIEYDPKHKQADEYRQLAMKVMNNTKLVIPTPIEMEELEDLLMEFGIM
                     EAEDESIVGKTAADLAA"
     gene            3664..>4454
                     /locus_tag="PKX01_16535"
     CDS             3664..>4454
                     /locus_tag="PKX01_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HMZ77794.1"
                     /translation="MSTMTREETDALIAEVLEVYPDKAKKDRKKHLASNDVTVEQSKK
                     CITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYVG
                     TTGVDTFGTMNFTSDFQEKDIVFGGDKKLAKLIEEIETLFPLHKGISVQSECPIGLIG
                     DDIESVSKKAAVAIEKPVVPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGQAYDST
                     PYDVAIIGDYNIGGDAWATRIILEEMGLRVVAQWSGDGTIAEMMNT"
BASE COUNT          836 a         1395 c         1408 g          815 t
ORIGIN      
        1 cccggccaac gataggcaaa acccgtacta ccggttcaac aagcgacgcc tgccccagtt
       61 ctgcgccacc gaagcccatc ggccaagcac acccggcccg cgccctactc cccccgactg
      121 cgcttcagac accccgccag cgctgcgtcc atcgttgcca ggtgggtggc gaaccagcct
      181 ggcagggcct ggatgtaggc gcgggccggg gtcaggcggc ccgcattgac accgcgggcg
      241 aagtgggcga attcgcccag tacgcgcagg tgttcgctgt tgtgctcacc gatggcaggg
      301 aagcggcagg ctttcatgag cttgccttcg tgctcgaagt gcaggcgggt gtgttcctgc
      361 aggctcagga agagcgcggg aaaggcagaa tcgtcggcgg acgcgagggc gtccacatgt
      421 tcgacgaatt cccggtgggt ggcgtccatg tccgccaccc ccagggattg ggcgtcgtcc
      481 cagggcatca gacggcgccg tgcatctcgc gcttggcgtt gttggtggac ttcaacgcgt
      541 cgtcgagggt cgtctcgggg tacttgaact tgtggccctt gaagccattg aagacggtga
      601 ccaccttctc ggcggcgctc tgacccttga agtcaccctt ttcgagggcc accaggtcga
      661 tcagttcgtt ccaggccatg ccctgcacca gggggatgaa ggccaccgtg ccttcgccga
      721 tcttggcgag gaaggggtcg tcgatgttga cagcgaagag caccgcgatg gtgtcggcct
      781 tgaggtcggc agcgtacttc tcgacgaagc cggggatgtg ctggagcgca tccagatggc
      841 cggagatgag cttgagctca tcgcccttgg cgagcacgac gacctgcttg aggacttcgt
      901 ggggcggctt gcggcggccc ttgtcgtcgg cgatctcgcc ttccttcagg accagttcgc
      961 cgcgataggc ttcccagccg gcctcgtcgg cggcttcgcg gaagttggcg ttgaagaagt
     1021 attgttcgta gcggtcgagc agcatgagat gggactccct ggattgaaag cggtcaggcg
     1081 ggctggggct cgccgatgag gtcgaagatg tcggcgtcga cgatttcgag accgatcttc
     1141 ttgcagtagc ggcgttcctt gtcggtgggc tcggcgatga agacccagcc agccggttcg
     1201 gcggcgctgt agatgatgtc cgccatcacc atgcgctcgg tgtcgcgatt gaggcgcagg
     1261 ccggccagca ggtattgctt gcccttgcgg aattccttga gctggggcgg gatggagaag
     1321 ccgcccatca gttcggtgat gaaatccacg tagtcggcgt cggaggcgat gtaggtgggc
     1381 tcgggccgcg gcgtgccgac gggcttgaag aggatgggat gttcgagctt gagctcttcg
     1441 gcgcgctggt agctgctgcc gtcccagaag aagatcttgt agcgcagggt ggtgccgccg
     1501 atgcgggcga tgccgacgat gaggttgtgg ggcacatcgg cgtaggagtc ctgcagctgg
     1561 gtgtcccggt tgatgtcgat gacgtagcgg ggacggatcg ccttgatcca gtcgtgcacc
     1621 gcgccgcggg tccattgcgt ctcgccgtag gtgcgggtca gaaacttgtt aaccgtgctg
     1681 cggccgcgct tgagctccac gttcatggcg gcgcggggga actcgtacat cagcttgggc
     1741 gccatgggct tgccgccgtt catggcaatg atgagggcgt cgctggtagc ggggatgggt
     1801 tcgccggtgg cgacgttctt gacgtcagcc agcacacctg cgcccaggta aggaacgatg
     1861 gaaccgtcct ggatgccggc cagcagcgtg gcgcgcagtt cgggggtcat ggaattctcc
     1921 ggagatgggg tatcggccct caccagcaat atccacgccc gcgagcaatc cctttataat
     1981 cagtttgtta gatccactcc ccaacgtcag gttcgtgcca ttgtggggtt tgtgacacga
     2041 ccgctacgcc ccctggcgtc ggcgtggaac gagggatccg gcagccgggc cccgagcgac
     2101 cggtcgtgga tttcatcccc cgctccacca cgcgcgtggg cgtggcacca cgcccccgaa
     2161 ccagccgcac gaggcgtccg acagaatgtg gggcacccgc gcgttcgcgc gagttccggc
     2221 gtaatcacct gtaagcccca tgcggtggcc gcgcgggcgc tgcaggcacg caatgcccag
     2281 gcacaccatt acgtcggctg tatacagatg tacgcccaac ccgcccgcac atgcgcgccg
     2341 ggcctgtccc cggcgtcgtc cgaacctgac ggaccgcctg cccgcaccgc aggcgcggtg
     2401 tcaatgcgtc cgggccattc aaggaggccc ggtgtcgtca accttcccca cacatccctt
     2461 tgtcgccacc cctacaaagc gtaggggcat tgtgggcttt gtgaggcttt gtcggatgac
     2521 gacacagccc actctcacac gtactttgaa tttatttcct tgcagatcag caagttagag
     2581 gctgtcagtc gacctggcac agtccatgcg ataggtgagt tgcgactctc actgtgtttc
     2641 atcgcaccaa tcgactcaca aatcgactga cagattaagg agattacacc atggctaaac
     2701 tgcgtcaatg cgccatctac ggcaagggcg gtatcggcaa gtccaccacc acccagaacc
     2761 tcgttgctgc cctcgccgag gccggcaaga aggtgatgat cgtgggttgc gacccgaagg
     2821 ctgactccac ccgtctgatc ctgcacagca aggcccagac caccgtgatg cacctggctg
     2881 ctgaagccgg ctccgtggaa gacctcgagc tcgaagacgt cctctccgtc ggcttcggcg
     2941 gcgtcaagtg cgtcgagtcc ggcggtccgg aacccggcgt cggctgcgcc ggccgtggcg
     3001 tgatcaccgc catcaacttc ctggaagaag aaggcgccta cgacgaagac ctggacttcg
     3061 tgttctacga cgtgctgggc gacgtggtct gcggtggctt cgccatgccg atccgtgaaa
     3121 acaaggctca ggaaatctac atcgtctgct ccggcgaaat gatggccatg tacgcggcca
     3181 acaacatctc caagggcatc gtgaagtacg ccaactccgg cggcgtgcgt ctgggcggcc
     3241 tgatctgcaa ctcccgcaac accgaccgcg aagatgaact gatcatcgcc ctggccgccc
     3301 gcctcggcac ccagatgatc cacttcgtgc cccgtgacaa cgcggtgcag cacgctgaaa
     3361 tccgtcgtat gaccgtcatt gaatacgatc cgaagcacaa gcaggccgac gaataccgtc
     3421 agctggccat gaaggtcatg aacaacacca agctggtcat cccgaccccg atcgagatgg
     3481 aagagctgga agacctgctg atggaattcg gcatcatgga agcggaagac gagtccatcg
     3541 tcggcaagac cgccgccgat ctggccgcct aatcaagcat ctgctgcacg gcgggtactc
     3601 cggtacccgc tgctttcaac cagcacgtct gccacagatt ggtggcggcg tcaggaggaa
     3661 aatatgagca ccatgactcg cgaagaaacc gatgccctca ttgccgaggt gcttgaggtt
     3721 tatcccgaca aggccaagaa ggaccgcaag aagcacctgg cgtccaacga cgtcaccgtc
     3781 gaacagtcca agaagtgcat cacgtccaac cgcaagtccc tgcccggcgt gatgaccatc
     3841 cgcggctgtg cctacgccgg ttccaagggt gtggtgtggg gtccgatcaa ggacatgatc
     3901 cacatctccc acggtcccgt cggctgtggc cagtattccc gcgccggtcg ccgcaactac
     3961 tacgtgggca ccaccggtgt cgacaccttc ggcacgatga acttcacctc cgacttccag
     4021 gagaaggaca tcgtcttcgg cggcgacaag aagctcgcca agctgatcga agaaatcgaa
     4081 accctgttcc cgctgcacaa gggcatctcc gtgcagtccg aatgcccgat cggcctgatc
     4141 ggtgacgaca tcgagtccgt ctccaagaag gcggccgtgg ccatcgaaaa gcccgtcgtg
     4201 ccggtgcgct gcgaaggctt ccgcggcgtc tcccagtccc tcggccacca catcgccaac
     4261 gacgcgatcc gtgactgggt cctcgacaag cgcgacggcc aggcctacga cagcaccccc
     4321 tacgatgtgg ccatcatcgg cgactacaac atcggcggcg atgcctgggc cacccgcatc
     4381 atcctcgaag aaatgggcct gcgcgtcgtg gcccagtggt ccggcgacgg caccatcgcc
     4441 gagatgatga acac
//
LOCUS       DAOQPA010000193         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_12669_length_4437_cov_18.083752, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000193 DAOQPA010000000
VERSION     DAOQPA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12669_length_4437_cov_18.083752"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKX01_16540"
     CDS             <1..832
                     /locus_tag="PKX01_16540"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMZ77795.1"
                     /translation="GPVAELPAVPAGAPELAGPASNDSAAPPTPDSATEAPPATPVEP
                     NAADVPAGGADLGELWAASGKVQYNVIRGERNFIIGRTTHAWTHDKEHYSMETVIETV
                     GLVGLIKPFRMVQRSEGRIGAHGLLPDKFTVERDGQVKERADFDWSTAKVVLMAGDRR
                     REFDLAEGDQDVLSLMHQLSLQPDGPMNAELLVVTGKSAVRSAIRNLGLEDLEVPAGR
                     VSAYHLGSTGHNGELKIDIWLAKDYANLPVRLKITDKNGDVLDQVATGVDLGPRADKK
                     "
     gene            903..2165
                     /locus_tag="PKX01_16545"
     CDS             903..2165
                     /locus_tag="PKX01_16545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HMZ77796.1"
                     /translation="MTITASQIQSASQALRAVLRFDYPADAVLSRYFRDLRELGARDR
                     AFVAEAVYGVLRRKRMLERLCGPEVSPRQLVLAWLARVEGLTLRQLGEGLHGRDTDFF
                     TAIKGRELPEGSLAETTDLPEWLAERLLATRTPEQVQALAQSLNRPAPLDLRVNPLKL
                     DRDALIEKLRADGIACAAAPLSPLAVRLEGKPALQKHPLFLDGSFEVQDEGSQLLGFL
                     VQPKRGEMVVDFCAGAGGKTLLLGALMRNSGRLYAFDVSDKRLAKLKPRVARSGLSNV
                     HPVVIASENDPRVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPESVAELTAKQA
                     AILASAARLVKAGGRLVYATCSLLPEENEAIVAAFLAAHPGFVQVSAGEILGKQGITV
                     GDAEQLHLSPAEHDTDGFFAAVLERSAG"
     gene            2162..2794
                     /locus_tag="PKX01_16550"
     CDS             2162..2794
                     /locus_tag="PKX01_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HMZ77797.1"
                     /translation="MIGRGALALLLACAVATADAQTPGVEPTRTVAMHGTAEVAFAPW
                     SDPEALLLQAIDDARDSIHVQAYLFTSKALARGLIAARRRGLRVEVLLDAEMNRPGSL
                     SVLPELLEAGVPVAMLTRFNIAHNKVMLFDADTPHAALATGSYNYTRSARLSNAENLL
                     ILRGNPALVRLYLDTWQRQRAEAVPLRSLDDLPARKDKRDGRESDRTTVD"
     gene            2760..4079
                     /locus_tag="PKX01_16555"
     CDS             2760..4079
                     /locus_tag="PKX01_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ77798.1"
                     /translation="MEENQIAQLLIDTWADLQEPAIFWQVGVLALCLLLAGLISRQLR
                     QIIARRRSVRDDAAAAGAGEEGLRRVIFPLTALVLVLLARIVLHKWHHVNLLKLAVPL
                     LLAGAAIRLIVHALKRAFPQAGWLASFERIFSTLAWLAVALHITGVLPTVIDGLEQIG
                     FSIGTAHVNLWMLFQGALTVLLTVLVALWVSGSIESRLMATEGLDANLKLVFARLAKA
                     LLLVLAVTIGLPLVGIDLTALSVFGGALGVGLGFGMQKIAANYVSGFILLLDRSIRLG
                     NLISVGTERGVVSQITTRYTVLKGMTGIESIVPNETLVGSVVQNETFTDPRVRIALPV
                     QVSYACDLERALAILVESAKTQPRVLTDPGPGAFVEAFADSGINLQLGFWIADPHEGT
                     LGIRSAINLEIWRRFKAEGIEIPFPQREVRLLNPPAPDVAPPPQSPA"
     gene            4179..>4437
                     /locus_tag="PKX01_16560"
     CDS             4179..>4437
                     /locus_tag="PKX01_16560"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ77799.1"
                     /translation="MSSGLIDLPWWGYIVVTLALTHVTIASVTIFLHRHQAHRALELH
                     PLPALFFRTWLWLTTGMVTKEWAAIHRKHHAKCETPDDPHSP"
BASE COUNT          677 a         1646 c         1406 g          708 t
ORIGIN      
        1 cgggcccgtc gcggagctgc cggccgttcc tgccggtgcg ccggagctgg ccggcccggc
       61 cagcaacgac tctgcggcgc cgcccacccc ggactccgcc accgaagccc cgcccgcgac
      121 gcccgtcgaa ccgaatgccg ccgacgtgcc ggccggcggc gccgacctgg gcgagctgtg
      181 ggccgcctcc ggcaaggtgc aatacaacgt gatccgcggc gagcgcaact tcatcatcgg
      241 ccgcaccacc cacgcgtgga cccatgacaa ggagcactac tccatggaga cggtgatcga
      301 gaccgtcggt ctggtggggc tcatcaaacc cttccgcatg gtccagcgca gcgagggccg
      361 catcggcgcc cacggcctgt tgccggacaa atttaccgtc gagcgggacg ggcaggtcaa
      421 ggagcgcgcc gatttcgact ggtccaccgc caaggtcgtg ctgatggccg gcgaccggcg
      481 gcgggaattc gatctggccg aaggcgacca ggacgtgctg tccctgatgc atcaactctc
      541 cctccagccg gacgggccga tgaacgccga gctgctggtc gtcaccggca agagcgcggt
      601 ccgttcggcg atccgcaatc tcggcctgga agacctggaa gtccccgcgg gccgggtttc
      661 ggcctaccat ctgggcagca ccggccacaa cggcgagctc aagatcgaca tctggctggc
      721 caaggattac gccaacctgc cggtccggct caagatcacc gacaagaacg gcgacgtcct
      781 cgaccaggtc gcgacgggcg tcgacctcgg ccctcgcgcc gataaaaagt aaaatccccc
      841 ctttcccggc tttgtccgtc ccgccgccgc ggcgggaccg ctcctttcag tatcggaagt
      901 ccatgaccat caccgcctcc cagatccagt ccgccagcca ggccctgcgt gccgtgctgc
      961 gcttcgatta ccccgccgac gcggtcctgt cccgctattt ccgcgacctg cgcgaactgg
     1021 gcgcccgcga tcgggccttc gtcgccgagg cggtgtatgg cgtgctgcgc cgcaagcgca
     1081 tgctggaacg cctgtgcggg ccggaagtct cgccccgcca actggtgctg gcctggctcg
     1141 cccgcgtcga aggcctcacg ttgcgccagc tcggtgaagg cctgcacggc cgcgataccg
     1201 actttttcac cgccatcaag ggtcgtgaac tgcccgaagg cagcctggcc gaaaccaccg
     1261 acctgcccga atggctggcc gaacgcctcc tcgccacgcg caccccggag caggtgcagg
     1321 ccctcgccca gagcctcaac cggcctgcgc cgctggacct gcgggtcaat cccctcaagc
     1381 tcgaccggga cgccctcatc gaaaaactgc gcgccgacgg catcgcctgc gccgccgcgc
     1441 ccctgtcgcc gctggccgtg cgcctggaag gcaagcccgc gctgcagaag catccgctct
     1501 tcctcgacgg cagtttcgag gtgcaggacg aaggcagcca gctgctgggc ttcctggtgc
     1561 agcccaagcg cggcgagatg gtcgtggatt tctgcgccgg cgccggcggc aagaccctgc
     1621 tgctgggcgc gctgatgcgc aactccgggc gcctctatgc cttcgacgtg tccgacaagc
     1681 gcctcgccaa actcaagccg cgggtggcgc gctccggcct gtccaacgtc catccggtgg
     1741 tcatcgccag cgagaacgat ccccgcgtca agcgcctcgc cggcaagatc gaccgggtgc
     1801 tggtggacgc gccgtgcagc ggcctcggca ccctgcgccg caacccggac ctcaaatggc
     1861 gtcagagccc cgagtccgtc gccgagctca ccgccaaaca ggccgccatc ctggccagcg
     1921 cggcgcgtct ggtgaaggcg ggcggccgcc tggtctacgc cacctgcagc ctgctgccag
     1981 aggagaacga agccatcgta gcggccttcc tggcggccca tccgggcttc gtgcaggtgt
     2041 cggccggcga gatcctgggt aagcagggca tcaccgtggg cgacgccgag cagctccacc
     2101 tgagcccggc cgagcacgac accgacggct tcttcgccgc ggtgctggag cgcagcgccg
     2161 gatgatcggc cgcggcgccc tggcgctcct gctggcctgc gcggtcgcca cggccgacgc
     2221 ccagacgccg ggcgtcgagc ccacccgcac ggtggcgatg cacggcaccg ccgaagttgc
     2281 ttttgccccg tggagcgatc ccgaggcgct gctgctccag gccatcgacg acgcccggga
     2341 ttccatccac gtgcaggcct atctcttcac cagcaaggcg ctggcgcggg gcctgatcgc
     2401 agcccggcgg cgcggcctgc gggtcgaggt gctgctcgac gccgagatga accgccccgg
     2461 ctccctcagc gtgctgcctg agctgctgga ggccggcgtg ccggtggcga tgctcacgcg
     2521 cttcaacatc gcccacaaca aggtgatgct cttcgacgcc gacacgcccc acgcggcgct
     2581 ggccaccggc tcctacaact acacccgctc tgcgcgcctg agcaacgcgg agaacctgct
     2641 gatcctgcgc ggcaaccccg cgctggtccg cctttacctg gatacctggc agcgccagcg
     2701 cgccgaggcc gtgcccctgc gcagcctcga cgacctgccc gcacggaagg acaaacggga
     2761 tggaagagaa tcagatcgca caactgttga ttgacacctg ggccgacctg caggagcccg
     2821 ccatcttctg gcaggtgggc gtgctggccc tgtgtctgct gctggccggg ctcatttccc
     2881 ggcagctgcg ccagatcatc gcccggcgcc gctccgtgcg ggacgacgct gctgccgccg
     2941 gggccggcga agagggcctg cggcgggtca tctttccact caccgcgctg gtgctggtgc
     3001 tcctcgcgcg catcgtgctg cacaagtggc atcacgtgaa tctgctcaag ctggcggtgc
     3061 ccctgctgct ggcgggggcg gcgatacggc tcatcgtgca tgccctcaag cgcgccttcc
     3121 cccaggccgg ctggctcgcg tccttcgagc ggatcttctc caccctggcg tggctcgcgg
     3181 tggccctgca catcaccggc gtgctgccca ccgtcatcga cgggctcgag cagatcgggt
     3241 tcagcatcgg caccgcgcac gtcaatctgt ggatgctctt ccagggcgcc ctcacggtgc
     3301 tcctcaccgt cctcgtggcc ctgtgggtgt ccggctccat cgaaagccgc ctgatggcca
     3361 cggaggggct cgacgccaac ctcaagctgg tctttgcccg cctcgccaag gccttgctgc
     3421 tggtgctggc ggtgaccatc ggcctgcccc tggtgggcat cgacctcacc gcgctttcgg
     3481 tgttcggcgg cgccctcggc gtgggcctgg gcttcggcat gcagaagatc gcggccaact
     3541 acgtgtccgg cttcatcctc ctgctggacc gctcgatccg cctgggcaac ctcatctcgg
     3601 tgggcaccga gcggggcgtg gtcagccaga tcaccacgcg ctacacggtg ctcaagggca
     3661 tgaccggcat cgagtccatc gtccccaacg agaccctggt gggctcggtg gtgcagaacg
     3721 aaaccttcac cgacccgcgc gtgcgcatcg ccctgcccgt gcaggtgagc tacgcctgcg
     3781 atctggaacg cgccctcgcg atcctggtcg aatctgccaa gacccagccg cgggtgctca
     3841 ccgatccggg gcccggcgcc ttcgtcgaag cctttgccga cagcggcatc aacctgcagc
     3901 tgggcttctg gatcgccgat ccccacgaag gcacgctggg catccgctcg gccatcaatc
     3961 tggagatctg gcggcgcttc aaggccgagg gcatcgagat tccctttccc cagcgggagg
     4021 tgcgtctgct caatccgcca gcgccggatg tggcccctcc gccacaatcg cccgcctgag
     4081 cgagcgctcg gcagggcttt tttcgggtct atattggatt ggcggagccc ctcgcttaga
     4141 atcgcgccca cgctcgaata taaggatgta ttgacatgct gtcttccggt ctgatcgatc
     4201 tgccctggtg gggatacatc gtcgtcacgc tggccctgac ccacgtcacc attgcctcgg
     4261 tgacgatctt cctgcaccgc catcaggccc accgcgccct ggaactgcac ccgctgccgg
     4321 ccctgttctt ccgcacctgg ctgtggctca ccaccggcat ggtcaccaag gaatgggctg
     4381 ccatccaccg caagcaccac gccaagtgcg aaaccccgga cgatccccac agccccc
//
LOCUS       DAOQPA010000194         4405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_12817_length_4405_cov_17.788046, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000194 DAOQPA010000000
VERSION     DAOQPA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12817_length_4405_cov_17.788046"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..653)
                     /locus_tag="PKX01_16565"
     CDS             complement(339..653)
                     /locus_tag="PKX01_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ77800.1"
                     /translation="MKKLVVVAAVAGLMGSTAAFADAGMDLAKAKNCLACHAVDKKVV
                     GPAYKDVAAKYKGDKTAEAKLVEKVQKGGSGVWGSMPMPPNPQVSPAEAKTLVAWVLS
                     QK"
     gene            complement(800..2539)
                     /locus_tag="PKX01_16570"
     CDS             complement(800..2539)
                     /locus_tag="PKX01_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ77801.1"
                     /translation="MPYRACLRLSTLCCALLGAGAALAARSDVTLVVADELRPLLDKH
                     LSVLQGEDEAGFDEVGRIALLRRARKEIGDLLATEGYFSPAVTGEAVTGGNFRILVTP
                     GPRTLVNQVDLRFHGALADPDAERIQRVAALRAAWSLPAGAPFRQADWDAAKQRLLDA
                     VSVRDFAAATITTSHARIDPEAATAALTVEVDSGPPFVFGALQVSGLQDYAEDLVRRF
                     NPPAPGEAYSLDRLLRFQSALQNTAYFASVIVDVDRQSDTPGAVPVRVQLTEAKPKRL
                     GFGAGVSSNTGYRVETSYRDANVFDRALSLVSGVRVEERRQLGYADVFLPPSSDGHLD
                     SVGALAEHTNIAGLQTQRQSIGVVRAMPRGNIETRVAFNYQHEILSPEGSLRSSRDAL
                     TANWSWTRRKVDNLLDPRNGHVLNVQVGGATQALLSDRNFARFYGRFQGFVPVARRDV
                     LILRGEGGITVANSRDGVPQDFLFRTGGAQSVRGYAYQSLGVKDGSATVGGRYLAVLS
                     SEYVHWYEGDWGVAAFFDAGNANDERQLFKMNYGYGLGPRWKSPAGPIALDLAYGQSD
                     HRVRLHFAVAIAF"
     gene            complement(2591..>4405)
                     /locus_tag="PKX01_16575"
     CDS             complement(2591..>4405)
                     /locus_tag="PKX01_16575"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMZ77802.1"
                     /translation="AAGWAATPAALRALCLERAAQALEARSHALVALLVREAGKTFAA
                     GLGEVREAVDFCRYYAARLRAGDLTGSAPLGPVVAISPWNFPLAIFCGQIVAALAAGN
                     PVIAKPAEQTPLIADQAVALLHEAGIPRGVLQCLPGPGATVGAALVADARVAAVMFTG
                     STEVATAIHRVLADRGDVPLIAETGGLNAMIVDSTALPEQVVTDVIASAFDSAGQRCS
                     ALRLLCLQDEIATRVLELLDGALGELRIGPPEDFATDIGPVIDAAAHLQLDAYVDALR
                     ADGHCVMTRSLPDVCTDGHFVMPTVIELRTPTELQREVFGPVLHVVRFALHDLDALLD
                     ALGRSGYGLTRGIQSRVDSTVERILGHGGVGNVYVNRNMIGAVVGVQAFGGEGLSGTG
                     PKAGGPLLLRRLVRRAPGLSLPGQCAPADLDTLDAWLTAGAGGVVDDAARARLRVRVA
                     ACRAATLWGAVLDLPGPTGERNTWRVHPRGMVLGLAEAVEDAVYQLAAALATGNRLAV
                     PAAAAGWLSALPEALRGCVALRPSDGFEPWQAALLAGAEGRVAAWRRQLAAQPGPLRP
                     ILVADPDYVLERLLVERCVTVNTAATGGNAGLMGLGES"
BASE COUNT          738 a         1538 c         1486 g          643 t
ORIGIN      
        1 ggtcacgttg gattgagcga aggcaccggt ggaagccagg ccagcgacgg ccagagcgat
       61 cagtttcttt tgcatatgtt tctcctcttg attgaagact ggcacgcacc gccgcagcgg
      121 ggccgaccaa tttcacctct ctaacgagaa gttggactgc attgtgtggg ggagggaaag
      181 ggttgggcaa ggacagcgtt gtttttaggc agcgcttgcc gcatcgagtg ttgcaactgc
      241 gcaacaggcc gaatggcagg catccccgcc cacgggtccc cctgcacaaa aaacagaagc
      301 cagcccgagg gctggcttct gtgtggccga aagaccgctt acttctggct cagcacccag
      361 gcgaccagcg tcttggcttc ggccgggctg acctgcgggt tcggcggcat gggcatgctg
      421 ccccacacac cggagccgcc cttctggacc ttttcgacca gcttggcttc ggcggtcttg
      481 tcgcccttgt acttggcagc cacatccttg taggcggggc cgacaacctt cttgtccacg
      541 gcgtggcagg ccagacagtt cttggccttg gccagatcca tgccggcatc ggcgaaggct
      601 gcggtggaac ccatcagacc ggcaacggcg gcaacaacta cgagcttctt cattgcattc
      661 ctctcggtgg tggcgaaaaa acgtatcaga ggagtctaat cctcatcccg ccggattgcc
      721 aattgtcctg cacaggccgg ccggcctccc ctcaacgtcc gggcaggtcg tggcgttgac
      781 gcccccggcg cacgtccgct cagaacgcaa tggcgaccgc gaaatgcagg cggactcgat
      841 gatcgctctg accataggcc agatcgagcg caatgggacc ggcgggactt ttccagcgcg
      901 gaccgaggcc atagccgtag ttcatcttga agagctggcg ctcgtcgttg gcgttgcccg
      961 cgtcgaagaa cgctgccacg ccccagtcgc cttcgtacca atggacatac tcggaactga
     1021 gcacggcgag gtagcgcccg cctacggtgg cgctgccgtc ctttacgccc aggctctggt
     1081 acgcatagcc ccgtacgctc tgggcgccgc cggtgcggaa caggaaatcc tggggcaccc
     1141 cgtcccgcga attggccacg gtgatgccgc cctccccgcg caggatcagc acatcccgcc
     1201 gcgcgaccgg cacgaagccc tggaagcggc catagaaacg ggcaaagttt cgatcggaca
     1261 gaagcgcctg ggtcgctccg cccacctgca cgttgagcac gtgaccattc cgcggatcga
     1321 gcaggttgtc caccttgcgg cgggtccacg accagttggc cgtcagcgcg tcccggctgc
     1381 tgcgcaggct gccctcgggc gacaggattt cgtgctggta attgaaggcg acgcgtgtct
     1441 cgatgttgcc gcggggcatg gcccgcacca cgccaatcga ctggcgctgg gtctgcagac
     1501 ccgcgatgtt cgtgtgctcg gccagcgccc cgacgctgtc caggtgccca tcggacgacg
     1561 gcggcagaaa cacgtcggcg taaccgagct ggcgacgctc ctccacgcgc accccgctga
     1621 cgaggctcag ggcacggtcg aacacattcg cgtcccggta gctcgtctcg acacgatagc
     1681 cggtattgct gctgacaccg gcaccgaaac ccaggcgctt gggcttggct tcggtcagtt
     1741 gtacgcgcac cggcaccgcc cccggcgtgt cgctctgccg atccacgtcc acgatcacgg
     1801 acgcaaagta ggcggtgttc tgcaatgcgc tctggaagcg cagcagccgg tcgaggctgt
     1861 aggcttcgcc gggggccggg gggttgaagc gccggacgag gtcctcggcg taatcctgca
     1921 gaccgctcac ctgcaaggcg ccaaacacga agggcgggcc ggaatcgacc tcgacggtca
     1981 gggcggccgt cgccgcctcc gggtcgatac gggcgtggct ggtggtgatc gtggctgccg
     2041 caaagtcgcg gacggacacc gcgtcgagga gacgctgctt ggcggcatcc cagtccgcct
     2101 ggcgaaacgg cgcgcccgcc ggcaggctcc aggccgcccg tagcgccgcc acccgctgga
     2161 tccgctcggc gtccggatcg gccagggcgc cgtgaaaacg tagatccacc tggttgacca
     2221 gggtgcgcgg tccaggcgtg acgaggatgc ggaagttgcc ccccgtcacc gcctcgccgg
     2281 tgacagccgg tgaaaagtaa ccttccgttg ccagcagatc gccaatctcc ttgcgggccc
     2341 gacgcagcaa cgcgatccgg ccgacttcat cgaaccccgc ctcgtcctcg ccctgcagga
     2401 cgctcagatg cttgtcgagc agcggccgga gctcgtctgc cacgacgagg gtgacatcgc
     2461 tccgcgccgc cagcgcggca cccgccccca gcagggcaca acagagcgtc gacaggcgta
     2521 ggcaggctcg atacggcacg ggcggattct aaggcctatc cgctccggag caccgcatgg
     2581 cgcgacggat tcagctttcc cctagcccca tcagcccggc gttgccgccg gtcgccgcgg
     2641 tgttgacggt gacgcaccgc tccaccagga gacgttccag gacgtagtct ggatcagcaa
     2701 ccaggatcgg gcggagcgga ccgggctggg cggccagctg ccgacgccag gccgccaccc
     2761 ggccctcggc gcccgcgagc agcgccgcct gccagggttc gaacccatcg gacggacgaa
     2821 gcgccacgca gccccgcagc gcctcaggca gggcagacag ccagcccgca gcggccgcgg
     2881 gaacggcgag gcggttgccg gtcgccaggg ccgccgccag ttggtacacg gcgtcctcga
     2941 ccgcttcggc caggcccagc accatcccgc ggggatggac gcgccaggta ttgcgctcac
     3001 cggtcggccc aggcagatcc agcacggcgc cccacagggt ggcggcccgg caggctgcca
     3061 cacggacccg cagccgggcg cgggccgcat cgtccacgac gcccccggcc cccgcggtca
     3121 accatgcgtc cagggtgtcg aggtccgctg gcgcgcactg accgggcaga gacaggccgg
     3181 gcgcccgccg caccaggcgc cgcagcaaca gggggccgcc ggccttgggt ccggtgcccg
     3241 agaggccctc gccgccaaag gcttgcacgc ccaccacggc gccgatcatg ttgcgattca
     3301 cgtagacgtt gccgacgccg ccgtgcccga gaatgcgttc gacagtgctg tcaacccgcg
     3361 actggatgcc gcgcgtgagc ccgtagccgc tgcgcccgag ggcatcgagc agtgcgtcca
     3421 gatcgtgcag ggcgaaccgg acgacatgca gcacgggacc aaagacttcg cgttgaagtt
     3481 cggtgggcgt gcgcagctcg atgacggtgg gcatcacaaa atgtccgtcg gtgcagacgt
     3541 cgggcaggct gcgggtcatg acgcagtgcc cgtccgcccg cagggcatcc acgtaggcat
     3601 ccagctgaag gtgggccgcg gcgtcgatga ccgggccgat gtcggtggcg aagtcctccg
     3661 gcggaccgat gcgcaattca cccagggcgc cgtcgaggag ttccagcacc cgcgtggcga
     3721 tttcgtcctg caggcacagc agacgcagcg ccgaacagcg ctgaccggcg gaatcgaagg
     3781 ccgacgcgat cacgtccgtc accacctgct ccggcagcgc ggtggaatcc acgatcatcg
     3841 cgttgagccc cccggtctcg gcgatcaggg gcacgtcgcc gcgatcagcc agcacccggt
     3901 ggatggcggt ggccacctct gtggagccgg tgaacatcac cgccgccacg cgggcatccg
     3961 ccaccagcgc agcccctacg gtcgcgcccg gcccgggcag gcattgcagc acgccacgcg
     4021 ggatgcccgc ttcgtggagc agcgccacgg cctggtcggc gatcagcggc gtctgctcgg
     4081 ccggtttggc gatcaccgga ttgccggcgg ccagggccgc cacgatctgg ccgcagaaga
     4141 tcgccagcgg aaagttccac gggctgatgg ccacgaccgg ccccagaggc gcgctgccgg
     4201 tcagatcgcc ggcgcgcagg cgggccgcgt agtagcggca gaagtctacc gcctcgcgga
     4261 cttcgccgag cccggcggcg aaggtcttgc cggcctcgcg cacgaggagc gccacgaggg
     4321 catggctgcg ggcctccagc gcctgggcag cgcgctccag gcacagggcg cgcagcgcgg
     4381 ccggcgtggc cgcccagccg gcagc
//
LOCUS       DAOQPA010000195         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_13000_length_4367_cov_16.499536, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000195 DAOQPA010000000
VERSION     DAOQPA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13000_length_4367_cov_16.499536"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKX01_16580"
     CDS             <1..298
                     /locus_tag="PKX01_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77803.1"
                     /translation="LAGGHNGAGAGAAVGMVAGTAAGASDARYAGADAQRRYDIAYQQ
                     CMYAKGNQLPGAYGYGSYRPASPYGTPPPAYPSGQPGYSIPPPNYPPPPPLRRE"
     gene            345..1508
                     /locus_tag="PKX01_16585"
     CDS             345..1508
                     /locus_tag="PKX01_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HMZ77804.1"
                     /translation="MKFDVLVVGAGLAGAALAAALRGCSLKVGVVEARAPSRPTGWDP
                     RIYAISPANVAFLTSIGIWGHLDAARMAPIHDMEIHGDAGGRLDFSAYDCGLGELAWI
                     VESSLMQLELWETIKRQHNVTLLCPAAPAALALDEDAARLTLADGRRIEARLIVAADG
                     RDSWVRQAAGIDVRNTPYEEKGVVANFRCEKPHRNTAFQWFRKDGVLAYLPLPAQQIS
                     IVWSAPDDLADQLVALSPEALAVRVAEATDARLGALETLTPAAAFPLRLMRVERVVQP
                     RLALIGDAAHAIHPLSGHGINLGYQDARVLAEQLAALPAWRDPGELAVLRPYARARAE
                     EAALLQYTTHGLNRLFKPANPLLSGLRNLGLNLTNRLPVLRNALVRYAIAGRF"
     gene            1541..2266
                     /locus_tag="PKX01_16590"
     CDS             1541..2266
                     /locus_tag="PKX01_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HMZ77805.1"
                     /translation="MKSASFRPLALALLLVTGSALANDEAAVRKNLDTFFGGQAVDTV
                     KKTPYGGLYEVVLKSGELVYTDAAVSFLLDGNVIDTKTKQNVTQARMAQLMKIDFATL
                     PLDQAIKQVRGTGKRVIATFEDPNCGYCKRLAKDMVNLKDATIYTFLYPILGPDSLEK
                     SKGIWCAKDRATAWTDWMTAGKAPAAGSCDTAVLDKNVALGQKMRVSGTPTIFTVDGN
                     RLPGAVPLAQLDKAMDQAATGKR"
     gene            complement(2303..3142)
                     /locus_tag="PKX01_16595"
     CDS             complement(2303..3142)
                     /locus_tag="PKX01_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ77806.1"
                     /translation="MPLTELVRHLNHRHALRGGEGRLDLVEGRAVLRVGDWLLGSEFQ
                     PVVDARSGHAIGHEARLAVQAGPAGLPSQALFLALEHVEAVIQVDRLCRHLHALNFLV
                     EPARRGFLSLNVHPCTAAHWGVHPVTVLDPVLMRCGLLADQLVLEFPDDGFGSALPLA
                     AAIDACRREGHRVALDNFGRHSADLRRVEALMPDIVKLDSSIIGHSGHLSLARRVLMG
                     LAAEFRRMGVVAVCQWIENPLQLQVARDVGVDWLQGRLLGRPTGRCVPVMPEIPLREP
                     AAA"
     gene            complement(3241..4296)
                     /locus_tag="PKX01_16600"
     CDS             complement(3241..4296)
                     /locus_tag="PKX01_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HMZ77807.1"
                     /translation="MAADWRDLPGWGSDAHDEAWPAFLQSCRSLGRQATWQGVCDAAK
                     GLGEKPSASAARSFFESRLTPWRVTNPDGTKEGLITGYYEPLIQGCRTRTAVCAWPIH
                     ATPDDLITVDLADAYPDLKPYRLRGRLVGRKVVPYYSRAELDRQPEKLAGKVLLYAAD
                     PVDLFFMQVQGSGRVQLPDGGLVRLAYADTNGHPYQSIGRWLADKGELRLDQASMDGI
                     KGWARANPGRLKEMLGANPSYIFFREVPNTPGGPAGALGLPLTDGRSIAIDPRSVPLG
                     APVFLATTHPRSAQPLQRLVLAQDTGTAIKGGVRADFFWGFGAEAGALAGRMKQKGEM
                     WVLWPRDQVPRAANGSL"
BASE COUNT          708 a         1676 c         1372 g          611 t
ORIGIN      
        1 cctcgccggc ggtcacaacg gcgccggtgc tggcgcggcc gtcggcatgg tggccggcac
       61 cgccgctggc gcctccgacg cccgctacgc cggcgcggac gcccagcgcc gttacgacat
      121 cgcctaccag cagtgcatgt acgccaaggg caaccagttg cccggcgcct acggatacgg
      181 cagctaccgc ccggccagcc cctacgggac gcccccgccc gcctatccaa gcgggcagcc
      241 cggctattcg atcccgccgc ccaattaccc gcccccgccg cccctgcgcc gcgagtgact
      301 tcccctgcgc cctccggcac aatccgggcg caggagaacc gtgcatgaaa tttgatgtcc
      361 tcgtcgtggg cgccggcctc gccggtgccg ccctcgccgc cgccttgcgc ggctgcagcc
      421 tcaaggtggg cgtggtggaa gcccgcgccc cgtcccggcc caccggctgg gatccgcgca
      481 tctacgccat cagccccgcc aacgtggcat tcctcacctc catcggcatc tgggggcacc
      541 tcgacgctgc gcgcatggcg ccgatccacg acatggagat ccacggcgac gccggcggac
      601 gcctcgactt ctccgcctac gactgcggcc tcggcgaact ggcctggatc gtcgaatcca
      661 gcctgatgca gcttgaactg tgggaaacca tcaagcgcca gcacaacgtc accctgctct
      721 gcccggccgc gccggcggcc ctggccttgg acgaggacgc cgcccgcctc accctggcgg
      781 atggccgccg catcgaggcg cgcctcatcg tcgcagccga cgggcgcgac tcctgggtgc
      841 gccaggccgc cggcatcgac gtccgcaaca cgccctatga agaaaaaggc gtcgtcgcca
      901 acttccgctg cgaaaagccc caccgcaaca ccgccttcca gtggttccgc aaggacggcg
      961 tgctcgccta cctgcccctg ccggcgcagc agatctccat cgtgtggtcc gccccggacg
     1021 acctcgccga ccagctcgtc gccctgtcgc cggaagccct cgccgtgcgc gtggccgaag
     1081 ccaccgacgc gcgcctcggc gccctcgaga ccctcacccc ggccgccgcc ttcccgctac
     1141 gcctgatgcg cgtggagcgc gtcgtgcagc cgcgtctggc cctcattggc gacgccgccc
     1201 acgccatcca tccgctgtcc ggccacggca tcaacctggg ctaccaggac gcccgcgtgc
     1261 tggccgaaca gctggcggcc ctgccggcct ggcgcgaccc cggcgaactg gccgtactgc
     1321 gcccctacgc ccgcgcccgc gccgaggaag ccgccctgct ccagtacacc acccacggcc
     1381 tcaaccgcct gttcaagccc gccaacccgc tcctgtccgg tctgcgcaac ctgggactga
     1441 acctgaccaa ccgtctgccg gtcctacgca acgcgctggt ccgctacgcc atcgccggcc
     1501 gtttctgacg gtcgtccgcc cttacccgga gattccctgg atgaagtctg cctcgttccg
     1561 ccccctcgcc ctcgccctgc tgctcgtcac cggcagcgcc ctcgccaacg acgaggccgc
     1621 cgtcaggaag aatctggaca cctttttcgg cggtcaggcc gtcgacacgg tcaagaagac
     1681 gccctacggc ggcctctacg aagtcgtgct caagtccggc gaactggtct acaccgacgc
     1741 ggcggtgagc tttctgctgg acggcaatgt catcgacacc aagaccaagc agaacgtcac
     1801 ccaggcgcgc atggcccagc tgatgaagat cgacttcgcc accctgcccc tcgaccaggc
     1861 catcaagcag gtccgcggca ccggcaagcg cgtcatcgcc accttcgagg atcccaactg
     1921 cggctattgc aagcgcctgg ccaaggacat ggtcaatctc aaggatgcga ccatctacac
     1981 cttcctgtac cccatcctcg ggcccgattc cctcgagaag tccaaaggca tctggtgcgc
     2041 caaggatcgt gccacggcat ggaccgactg gatgaccgcc ggcaaggccc ccgccgcggg
     2101 ctcctgcgac accgccgtac tcgacaagaa tgtggccctg ggccagaaga tgcgggtgag
     2161 cggcacgccc accatcttca ccgtggatgg caaccgcctg ccgggcgcgg tacccctcgc
     2221 ccagctcgac aaggccatgg atcaggccgc taccggcaag cgctgatccg gcaaacgccc
     2281 gctcggcagg ccgacgcctc gatcaggcgg cggccggctc acgcagcggg atctccggca
     2341 tcaccggtac gcagcggccc gtcggccgac cgagcaggcg gccttgcagc cagtccacac
     2401 ccacgtcccg cgccacctgg agctgcagcg gattctcgat ccactggcac accgccacca
     2461 cgcccatgcg gcggaactcc gccgccagcc ccatcagcac gcgccgcgcc aggctcaggt
     2521 ggccggagtg gccgatgatg ctgctgtcga gcttgacgat gtcgggcatc agcgcttcga
     2581 cgcgacgcag gtcggccgag tggcgcccga aattgtccaa ggccacgcga tggccttccc
     2641 gccggcacgc gtcaatcgcc gccgccagcg gcagcgcgct gccgaagccg tcatccggga
     2701 attccagtac cagctgatcc gccagcagtc cgcaacgcat cagcacggga tcgagcaccg
     2761 tcaccggatg aacgccccag tgcgccgccg tgcacggatg gacgttgagg gacaggaagc
     2821 ccctgcgcgc cggctctacc agaaagttga gggcatgcag gtgacggcac aggcgatcca
     2881 cctggatcac cgcctccaca tgctccaacg ccaggaacag ggcctgggac ggcagccccg
     2941 ctggcccggc ctgcacggcg aggcgcgcct cgtgaccaat ggcgtggcca ctgcgtgcat
     3001 ccaccaccgg ctggaattcc gatcccagca gccagtcgcc cacacgcagc acggcgcgcc
     3061 cctccacaag atcgagccga ccttcgcccc cgcgcagggc gtgacggtgg ttgagatggc
     3121 ggacgagttc ggtgaggggc atgaaggtct ccgtgaaggc cggctgatga cacgggcacg
     3181 agcctactct ccggccatga cgccggatcg cggcagaacc atcaatttac gaaaaggatg
     3241 ttacagcgat ccgttcgctg cccgcggcac ctgatcccgg ggccacagca cccacatttc
     3301 gcccttctgc ttcatccggc cggccagggc accggcctcg gcgccgaagc cccagaagaa
     3361 gtccgcacgc accccgccct tgatcgccgt gcccgtgtcc tgggccagta ccaggcgctg
     3421 caggggctgg gcgctgcgcg ggtgggtggt ggccaggaac accggcgccc ccaagggcac
     3481 actgcgcggg tcgatggcga tgctgcgacc gtccgtgagg ggcaggccca gcgctccggc
     3541 cggaccgccc ggcgtgttgg gcacctcgcg gaagaagatg tagctcggat tggcgccgag
     3601 catctccttc agccgtccgg gattggccct ggcccagccc ttgatgccat ccatcgacgc
     3661 ctgatcaagg cgtagctcgc ccttgtccgc cagccagcgg ccgatggact gatagggatg
     3721 accgttggtg tcggcgtagg ccagccgcac cagccccccg tcgggcagct gcacccgccc
     3781 cgagccctgc acctgcatga agaacagatc caccggatcg gccgcgtaga gcagcacctt
     3841 gccggccagc ttttccggct ggcggtccag ctcggcgcgg gagtaataag gcaccacctt
     3901 gcgccccacc aggcgcccgc gcaggcgata gggcttgaga tcgggatagg cgtcggccag
     3961 gtccacggtg atcaggtcgt ccggtgtcgc gtgaatcggc caggcgcaaa cggctgtgcg
     4021 ggtcctgcag ccctggatca gcggttcgta atagccggtg atgaggcctt ccttcgtacc
     4081 atccggattg gtgacccgcc agggcgtcaa gcggctctcg aagaagctgc gcgcggccga
     4141 cgccgagggc ttttcaccga gccccttcgc cgcgtcgcag accccttgcc aggtggcctg
     4201 ccgaccgaga ctgcggcacg actggagaaa ggccggccag gcctcgtcgt gggcgtcgct
     4261 gccccagccg ggcagatcgc gccagtctgc cgccagcagc gggcgcgcca ggggcgtggt
     4321 gggcgccgcg gccgccccgg gtggagccgg gacggcaggg gccgccg
//
LOCUS       DAOQPA010000196         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_13579_length_4247_cov_18.977338, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000196 DAOQPA010000000
VERSION     DAOQPA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_4247_cov_18.977338"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="soxB"
                     /locus_tag="PKX01_16605"
     CDS             <1..1120
                     /gene="soxB"
                     /locus_tag="PKX01_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HMZ77808.1"
                     /translation="NIKTADFGDPVFKPYVIRPINGVPVAIVGQAFPYTPIANPRWQV
                     PNWRYGIQDDALQVVVDEARAAGAQVVVVLSHNGMDVDLKMAGRVRGVDAILGGHTHD
                     GVPAPVIVPNAGGRTLVTNAGSNGKYLGVLDLEVRDGRVTDFRYKLLPVFANLIPADP
                     AMAALIDRLRAPYAARLAEPLAVTEALLYRRGNFNGSWDQLILDALLAEKNAEIAFSP
                     GFRWGTSLLPGDTLRMEHLLDQTAITYPWTTLTSMSGAQIKTVLEDVCDNLFNPDPYY
                     QQGGDMVRVGGLQYTCDPNARMGSRILDMRLHGQPLQADKQYKVAGWAPVSEEARNTG
                     GEPVWDLVARYLKGQKVLRARPLNLPTLRGMAGNPGIA"
     gene            1132..2094
                     /locus_tag="PKX01_16610"
     CDS             1132..2094
                     /locus_tag="PKX01_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ77809.1"
                     /translation="MTRFRRTLPPALRVIVALTLACLNGWAAAMDLVPLRVSEHVWYV
                     RGDSGVASAANQGFNSNAGFVVTADSVVVVDALGTPALGEALLTAIRRISTLPVRRVV
                     LTHFHADHFYGLPAFKAVGAEVWAHRAGRAYFASGEAEARLAQRRRDLFPWVDEHTTL
                     IQADRWLDGPVRFSDGGLHFRIDILGPAHSPEDLTLTVEEDGVVFCGDILFAGRIPFV
                     GNADSRRWLAAIDAVAALHPRILVTGHGPHSSDAAADLSLTGDYLRFLREKMGRAVAD
                     MVPFDEAYAQTDWGRFRALPAFDAANRINAYGTYLTLERESLGQ"
     gene            2091..2564
                     /locus_tag="PKX01_16615"
     CDS             2091..2564
                     /locus_tag="PKX01_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77810.1"
                     /translation="MKSLLRIFAAILLGVVVPAVAGKGSAALSSDLHEPDARRDDLLY
                     VVLFSLPECPYCVAVRQAYLPALPEDPRFRERVRIREVRIGDPRTLSGFDGAPVSHAA
                     LAKRYGVKFAPTVLFLDAAGQPRAEPLLGGDSVGLYGGYLEKRVMQGLGLLPRPD"
     gene            complement(2571..2870)
                     /locus_tag="PKX01_16620"
     CDS             complement(2571..2870)
                     /locus_tag="PKX01_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ77811.1"
                     /translation="MWKIRLTCAIACLGAALAQAADPVLGEKLAREHRCADCHRRLIG
                     GDGSALYTRPDRKVTSRDKLTAQVSFCSSQLKTGWFPEEEEAVAAWLNQRYYHFR"
     gene            3034..3522
                     /locus_tag="PKX01_16625"
     CDS             3034..3522
                     /locus_tag="PKX01_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ77812.1"
                     /translation="MALYLSAHEAWERMQQAPAKVLFLDVRTRAEAMYVGMPTAVDAL
                     VPYMEHQEIMSDWDERRHMYLLLPNSDFVQEAVARLQAKGLTRSDPVILICRSGDRSA
                     RAADLLAGAGFTQVYSVVEGFEGDLAKSGPRVGQRVVNGWKNADLPWSYQLDKAKMYF
                     PR"
     gene            3762..>4247
                     /locus_tag="PKX01_16630"
     CDS             3762..>4247
                     /locus_tag="PKX01_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ77813.1"
                     /translation="MSETQAQTAKAPDRPRKKAGNDTEETLYAVRQKVYPRAVTGTFA
                     AWRWTLVWLTQILFYGLCWVPWNGRQAVLLDLVNRKFYIFGWIFWPQDVFFLAVLLII
                     CAYSLFFFTAVAGRLWCGYSCPQTVYTEIFMWIERKIEGDRTQRMKLDQAPMSARKLG
                     LK"
BASE COUNT          648 a         1465 c         1420 g          714 t
ORIGIN      
        1 gaacatcaag accgccgact tcggcgaccc ggtgttcaag ccctacgtga tccgccccat
       61 caacggcgtg cccgtggcca tcgttggcca ggccttcccc tacaccccca tcgccaaccc
      121 ccgctggcag gtgcccaact ggcgctacgg catccaggac gatgcccttc aggtcgtcgt
      181 ggacgaagcc cgcgcggccg gcgcccaggt ggtggtggtg ctgtcccaca acggcatgga
      241 cgtggatctc aagatggccg gccgggtgcg cggcgtggac gccatcctcg gcggccacac
      301 ccacgacggc gtgcccgcgc cggtaatcgt gccgaacgcg ggtggccgga cgctggtcac
      361 caacgccggc tccaacggca agtacctggg cgtgctcgac ctggaggtga gggacggccg
      421 cgtcacggat ttccgctaca agctgctgcc cgtcttcgcc aacctgatcc ccgccgaccc
      481 ggccatggcc gcgctcatcg accggctgcg cgcgccctac gcggcccggc tcgccgagcc
      541 gctggccgtc accgaggcgc tgctgtaccg ccgcggcaac ttcaacggct cctgggacca
      601 gctcatcctc gacgccctgc ttgccgagaa aaacgccgag atcgccttct caccgggttt
      661 ccgctggggg acctcgctgc tgcctggcga caccctgcgc atggagcacc tgctggacca
      721 gaccgccatc acctacccgt ggaccacgct cacgtcgatg agcggcgcgc agatcaagac
      781 cgtgctggaa gacgtctgcg acaacctctt caacccggac ccctactacc agcagggcgg
      841 cgacatggtg cgtgtgggcg gcctgcaata cacctgcgat cccaacgccc gcatgggcag
      901 ccgcatcctc gacatgcgcc tgcacggcca gcccctgcag gccgacaagc agtacaaggt
      961 ggccggctgg gcgcccgtgt ccgaagaggc ccgcaacacc ggcggcgaac ccgtgtggga
     1021 tctggtggcc cgttacctca agggccagaa ggtgctgcgc gcgcgccccc tcaacctgcc
     1081 cacactgcgg ggcatggccg gcaatcctgg catcgcttga ggcgtccgac gatgacccgc
     1141 ttccggcgca ccctccctcc cgcgctgcgg gtcatcgtcg ccctcaccct ggcgtgtctg
     1201 aacggctggg ccgcggccat ggacctcgtg ccgctgcgtg tctccgagca cgtgtggtac
     1261 gtgcgcggcg attccggcgt ggcctcggcg gccaaccagg gcttcaacag caacgccggc
     1321 ttcgtggtca ccgccgacag cgtcgtcgtc gtggatgccc tgggtacgcc ggccctgggc
     1381 gaggccctgc tcacggcgat ccgccgcatc tccacgctgc cggtgcgacg ggtcgtgctg
     1441 acccacttcc acgccgatca tttttacggc ctgccggcct tcaaggcggt cggcgccgag
     1501 gtgtgggccc accgtgccgg acgcgcgtat ttcgccagcg gtgaggcgga ggcgcgtctg
     1561 gcccagcgcc ggcgcgacct ctttccgtgg gtggacgagc acaccacgct gatccaggcc
     1621 gatcgctggc tggacggccc ggtgcgcttc agcgacggcg ggctgcactt ccgcatcgac
     1681 atcctgggac cggcccattc ccccgaggat ctgaccctga ccgtcgagga ggacggcgtg
     1741 gtgttctgcg gcgacatcct cttcgccggg cgcattccct tcgtcggcaa cgccgacagc
     1801 cggcgctggc tggcggccat cgacgccgtt gcggccctgc atccgcgcat cctggttacc
     1861 ggccacgggc cccattcgag cgatgcggcg gcggacctga gcctcaccgg cgactacctg
     1921 cgcttcctgc gggagaagat gggccgcgcc gtggcggaca tggtgccctt cgacgaggcc
     1981 tacgcgcaga cggactgggg ccgcttccgg gcgctgccgg ccttcgatgc ggccaatcgc
     2041 atcaacgctt acggcaccta cctgaccctc gaacgggagt ccctcggcca gtgaagtcgc
     2101 tgctgcggat cttcgccgcg attctcctcg gggtggtggt gccggcggtg gcgggcaaag
     2161 gcagtgcggc cctgtcgtcc gatctgcacg agcccgacgc ccggcgcgac gatctgctct
     2221 acgtcgtgct cttcagcctg ccggagtgcc cgtactgcgt tgccgtgcga caggcgtatc
     2281 tgccggccct gccggaggac ccccgcttcc gtgagcgggt gcgcatccgg gaggtgcgca
     2341 tcggcgatcc gcgcacgctg agcggcttcg acggagcgcc ggtgagtcat gcagcgctgg
     2401 cgaagcgcta tggcgtgaag tttgcaccca cggtgctgtt cctcgacgcg gccggccagc
     2461 cccgcgccga gcccctgctg gggggcgaca gtgtgggcct gtacggcggc tatctggaaa
     2521 agcgtgtgat gcagggcctg ggcctgctgc cgcggccgga ttaggggaga ttacctgaag
     2581 tggtaatacc gctggttcag ccaggctgcg accgcttcct cctcctcagg gaaccagccg
     2641 gtcttgagct ggctgctgca gaagctgacc tgggcggtga gtttgtcgcg gctggtgacc
     2701 ttgcggtccg gccgcgtgta tagggccgag ccgtcgccgc cgatgaggcg ccggtggcag
     2761 tccgcgcagc gatgctcgcg ggcgagcttt tctcccagga ccgggtcggc ggcctgggcc
     2821 agggcggcgc ccaggcaggc gatggcgcag gtcagacgga ttttccacat cggcagggtc
     2881 tccggactgg gccgatccat cccctcgaaa ggacgaatcg tgagcaaacg gctgttggcg
     2941 tgtatctgca ctctggggct gctcctggcg cccctttcca cgatagcagc tgcggtcgat
     3001 cccgccacgc tgccggaagc gaaacggacc cgtttggccc tctacctgag cgcccatgag
     3061 gcctgggagc ggatgcagca ggcgccggcc aaggtgctct tcctggacgt gcggacgcgg
     3121 gccgaggcca tgtacgtggg catgccgacg gccgttgacg cgctggtgcc gtacatggag
     3181 caccaggaga tcatgtccga ctgggacgag cgccgtcaca tgtacctgct gctccccaac
     3241 agcgacttcg tccaggaggc ggtcgcccgc ctgcaggcca aaggcctcac ccggagcgat
     3301 ccggtgatcc tcatctgccg gtccggcgac cgcagcgcac gggcggcgga cctgctggcc
     3361 ggggccggct tcacccaggt gtacagcgtc gtcgagggct tcgagggcga tctggcgaaa
     3421 tccggcccgc gggtcgggca gcgcgtcgtg aacggctgga agaacgcaga cttgccgtgg
     3481 tcctaccagc tggacaaggc gaagatgtac tttccgcgct gagggatcgt cagcagccct
     3541 ggcaggactg acagtgctgt cggtcctggc agatcgtggc agtccggact gccgtttcga
     3601 gatcgaaagg cgctggcggg attgcgggaa tttcaggcaa gcctttgata tcaaataaat
     3661 gatccgtctg cggggtggct ggtacggata ttgattggac ctgggctgac aggtcgtcca
     3721 gtaccggacg acgatccgat ttcccttgca ggtcatcagc catgagcgaa acccaagccc
     3781 aaaccgcgaa agccccggat cgcccccgca agaaagccgg gaacgacacc gaagagaccc
     3841 tctacgccgt ccgccagaaa gtctatcccc gcgccgtgac cggcaccttc gccgcgtggc
     3901 ggtggacgct ggtgtggctc acccagatcc tcttctacgg cctctgctgg gtgccctgga
     3961 acgggcggca ggcggtgctg ctggatctgg tcaatcgcaa gttctacatc ttcggctgga
     4021 tcttctggcc ccaggacgtg ttcttcctgg ctgtgctgct gatcatctgc gcgtattccc
     4081 tgttcttctt caccgcggtc gccgggcgat tgtggtgcgg ctatagctgt ccgcagacgg
     4141 tctacaccga gatcttcatg tggatcgagc gcaagatcga gggtgaccgg acccagcgca
     4201 tgaagctcga ccaggcgccc atgagcgccc gcaagctcgg actcaag
//
LOCUS       DAOQPA010000197         4223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_13711_length_4223_cov_14.083253, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000197 DAOQPA010000000
VERSION     DAOQPA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13711_length_4223_cov_14.083253"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1823
                     /locus_tag="PKX01_16635"
     CDS             72..1823
                     /locus_tag="PKX01_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ77814.1"
                     /translation="MALPVTGSSSTDERQADIDLVVVGAGAGGMVAALVAALEGLRVV
                     LCEGSQQVGGTTATSAGTLWIPGNRHGAEAGHADSVQAAASYLDALIGPDDGRQLRQA
                     FLESGDAAIAYLEERTDVAFVSAGQHPDYLALPGAAIAGRALAPRPFDGRLLRGAFAR
                     IRPPMPEFLLLGGMMVGKADIQALVRRWQSWPAFVASAGLVARYLVDRIRYPRGTRLV
                     MGNALVARLFYSLQRAGVDVRFGWRLRELTVEGGKVRGATFAVDGQERRLDSRAGVVL
                     ATGGVGHGEALRAELAPAGMSLPSLACDAVNGEGITAARSAGAGFERYAAGDFFWQPV
                     SRVPRPGGPGLFPHLFLDRAKPGLVAVDAQGRRFADEAGSYHHFVEAMVRHLQRTGGD
                     AAWLVCDAAFVHKYGLGVILPGTRRLDRWIRQGYVCVADRLPALAQRAGIDPAGLLAT
                     VQRTNDYAATGRDPDFHKGDAPVDRFNGDPAHRPNPCLGEIGQPPFVALRILPADAAS
                     SSGLPIDADGRVLDRAGRVVVGLYACGNDAASVMRGTYPGPGTTLGPAFVFAWRAALF
                     AARRAASARAHPIEEKP"
     gene            1823..2932
                     /locus_tag="PKX01_16640"
     CDS             1823..2932
                     /locus_tag="PKX01_16640"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HMZ77815.1"
                     /translation="MEPNASPARRAGPLADLRVVEFAGIGPGPFACMLLSDMGADVVT
                     IDRPGARASDPRNVTQRGRTVVQADLKDAACIEQVLQLLQAADVLVEGFRPGVMERLG
                     LGPEAVARRNARLVYGRMTGWGQHGPLAQAAGHDINYIALTGALHAIGTADRPVPPLN
                     LLGDYGGGSLYLVMGILAALHEARRSGLGQVVDAAITDGVVNLMAQFIGQSQRGAFVE
                     RRESNMPDGGWPWYGVYETADGPHVSVGPLEPQFFARLCELLQLGPEWQSAPSDRSRW
                     PALREALAQLFKSRPAAHWRELLEGTDACFAPVLPLSEAARHPHNVARGAFVVLDGVT
                     HPAPAPRFSRTPAAIQSAPAAAAGDLADALGRWRG"
     gene            complement(2873..3694)
                     /locus_tag="PKX01_16645"
     CDS             complement(2873..3694)
                     /locus_tag="PKX01_16645"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HMZ77816.1"
                     /translation="MNSPTPAFAAVAPARAADEIARQIRELVASGQIKPGDRLPSERD
                     LAQRLHVSRNTLREALRALEHAGMIEMRKGAAGGAFVLPGDSGAIVTGLKDLYHLGAI
                     TPQQLTDARIWLSDVVVRVACQRATEEDFEQFEANVAASAQAQRAGDFGTRQRLNREF
                     HVLLARATRNPIMASMMEALMEVMGEFIGRIGPSDNPFVLPSRRRLLRHLRARDEKAA
                     VAEIVTSLTRLQDKYLALADRQRAQAASTRDATAAALAAPTAKRVRQVPGGSGRR"
     gene            complement(3761..>4223)
                     /locus_tag="PKX01_16650"
     CDS             complement(3761..>4223)
                     /locus_tag="PKX01_16650"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ77817.1"
                     /translation="VRAFMALNQAVLYEGTVPHETKMLVSLASSYASGCLYCQSHMAN
                     LSSIYKASDEKIAALWNFEESELFTPAERAAVRLALKAAKQPNEATQADFDALKEHFD
                     DGQIVEIVASIALFGYLNRWNDTMATELEPLAVEVAERTIGPVGWEPGKHG"
BASE COUNT          555 a         1444 c         1538 g          686 t
ORIGIN      
        1 cgcatcgggg ccgaagttca gggcgtgttc gagcatcacc cggaggcttc gcagccgtac
       61 acgctgctgc agtggcgctg ccggtgacgg ggtcgtcttc gacggatgaa aggcaagccg
      121 atatcgacct ggtcgtggtc ggtgccgggg cgggcggcat ggtggccgcg ctggtggcgg
      181 cgctcgaagg cctgcgcgtc gtgctgtgcg agggctcaca acaagtcgga ggcacgacgg
      241 ccacttccgc cggcacgctc tggatcccgg gcaaccggca cggggcggag gcgggccatg
      301 cagactcggt ccaggcggcg gccagctacc tggacgcgct gatcgggccg gacgatgggc
      361 gccagctgcg gcaggcgttc ctggaatccg gcgacgcggc cattgcctac ctcgaggagc
      421 gcaccgacgt cgccttcgtc agtgcgggac agcatccgga ctacctggct ttgcccggtg
      481 cggcgatcgc tggccgggcg ctggcgccgc gccccttcga cggacggctg ctgcgtggcg
      541 cattcgcgcg gatccggccg ccgatgccgg agttcctgct gctcggcggg atgatggtgg
      601 gcaaggccga catccaggca ctggtgcggc gctggcaatc ctggcccgcg ttcgttgcca
      661 gtgccgggct ggtggcccgc taccttgtcg accgcatccg ctatccccgc ggcacgcgcc
      721 tggtgatggg caatgcgctg gtcgcccgcc tgttctacag cctgcagcgc gccggcgtcg
      781 acgttcgctt cggctggcgc ctgcgggaac tgaccgtcga aggcggcaag gtgcggggag
      841 ccacctttgc cgtcgatggc caggagcggc gcctcgacag ccgggcgggc gtggtcctgg
      901 ccaccggtgg cgttggtcac ggcgaggccc tgcgcgccga actggctcct gccggaatgt
      961 ccttgccttc gctggcttgc gatgcagtca acggcgaagg catcaccgcg gcgcgatccg
     1021 ccggcgccgg gttcgagcgc tacgccgccg gcgacttctt ctggcagccg gtgtcgcgcg
     1081 tgccacgccc aggtgggcca gggctttttc cccatctttt cctggaccgc gccaagccgg
     1141 gcctggttgc cgtggacgcc cagggacgca ggttcgccga cgaagcgggt tcgtaccacc
     1201 acttcgtgga ggcgatggtg cgccacctgc agcgcaccgg cggcgatgcg gcctggctgg
     1261 tctgcgatgc ggccttcgtg cacaagtacg gactgggcgt gatcctgccg gggacgcgcc
     1321 ggctggatcg ctggatccgc caaggctatg tctgcgtggc cgaccgcctg ccggcgctgg
     1381 cgcagcgcgc cggcatcgat cccgcggggc tgctcgccac cgtgcagcgg accaacgact
     1441 atgctgcaac tggccgtgac ccggacttcc acaagggcga tgcgccggtc gaccggttca
     1501 acggcgaccc ggcccaccga ccgaaccctt gcctgggcga aatcggccaa ccgcccttcg
     1561 tcgcgttgcg catcctgccg gccgacgcgg cttcgagttc ggggctgccg atcgatgccg
     1621 atggccgggt gctggaccgc gctggccgtg tcgtcgtagg actctatgcc tgcggcaacg
     1681 atgccgcgtc cgtgatgcgg ggcacctatc cgggcccggg aacgacactg gggccggcct
     1741 tcgtctttgc ctggcgggcc gcgctgtttg cggcgcgtcg tgctgcatcg gcgcgtgcgc
     1801 atccgatcga ggagaaaccg tgatggaacc gaatgcttcc cccgcgcgcc gcgcgggccc
     1861 gctcgcggac ttgcgggtcg tcgagttcgc gggcatcgga ccggggccct tcgcctgcat
     1921 gctgctgtcg gacatgggcg ccgacgtggt gacgatcgac cgcccggggg cacgcgccag
     1981 tgatccgcgc aacgtcacgc agcggggacg cacggtggtg caggccgacc tcaaggacgc
     2041 cgcctgcatc gagcaggtgc tgcagctgct gcaggcggcc gacgtcctgg tggaaggctt
     2101 ccgcccgggc gtcatggagc gcctggggct ggggccggag gcggtcgcgc gtcgcaacgc
     2161 gcggctggtc tacggccgaa tgacgggctg gggccagcat gggccgctgg cgcaggccgc
     2221 ggggcacgac atcaactaca tcgcgctcac cggtgcgctg cacgccatcg gcacggccga
     2281 ccggccggtg ccgccgctga acctgctggg ggactacggc gggggcagcc tctacctggt
     2341 catgggcatc ctggccgcat tgcacgaggc gcggcggtct gggctggggc aggtggtgga
     2401 tgcggcgatc accgacggcg tcgtcaacct gatggcgcag ttcatcggcc aatcccagcg
     2461 cggtgccttc gtcgaacgcc gcgaaagcaa catgccggac ggcggatggc cgtggtacgg
     2521 ggtgtacgaa accgccgatg gaccgcacgt gagcgtcggg ccgctggagc cgcagttctt
     2581 cgctcgcttg tgcgagttgc tgcaactggg tcccgagtgg cagtccgcgc cctcggaccg
     2641 ttcgcgctgg ccggccctgc gcgaggcgct ggcgcagctc ttcaagagcc gccccgcggc
     2701 ccattggcgc gagctgctgg aaggaaccga cgcctgcttc gcgcccgtgc tgccgctcag
     2761 cgaagccgcc cggcacccgc acaacgtcgc gcgtggcgca ttcgtggtgc tggatggcgt
     2821 cacgcatccc gcaccggcgc cgcgcttctc gcggacgccg gcagccatcc agtcagcgcc
     2881 ggccgctgcc gccggggacc tggcggacgc gcttggccgt tggcgcggct agcgcagccg
     2941 ccgttgcatc gcgggtgctg gccgcctggg cccgctgccg gtcggccagc gcgaggtact
     3001 tgtcctgcag ccgggtcagg ctggtgacga tctcggccac ggccgccttt tcgtcccggg
     3061 cccgcaggtg gcgcaggagc cgccgccgcg acggcagcac gaaggggttg tcgctcgggc
     3121 cgatgcgccc gatgaactcc cccatcacct ccatcagcgc ttccatcatg ctggccatga
     3181 tggggttgcg ggtggcgcgc gccagcagca cgtggaactc gcggttcagg cgctggcggg
     3241 tgccgaagtc accggcgcgc tgcgcttgcg ccgaggcggc gacgttggcc tcgaactgct
     3301 cgaagtcctc ctcggtcgcg cgctggcagg ccacgcggac cacgacgtcg ctgagccata
     3361 tacgcgcatc ggtcagctgt tgcggcgtga tcgcgccgag gtgatagagg tctttcagcc
     3421 ccgtgacgat ggcgccggag tcgccgggca gtacgaaggc gccacccgcc gcgcccttgc
     3481 gcatctcgat catcccggcg tgctccaggg cccgcaacgc ctcgcgcaag gtgttgcggc
     3541 tcacgtgcag tcgctgtgcc aggtcgcgct cggaaggcag gcggtcgcca ggcttgatct
     3601 ggccgctggc gacgagctcc cggatctgcc gcgctatctc gtcggcggca cgggccggag
     3661 caacggcagc gaaggcggga gtcggcgagt tcaagggaag cagtcgtgtc cttcgattct
     3721 agccagccga ggtcgatgat ccggccagcg atcgtcccct tcagccgtgc ttgcccggct
     3781 cccaccccac cggcccgatg gtgcgctcgg ccacttccac cgccagcggc tcgagttcgg
     3841 tggccatcgt gtcgttccat cggttgaggt aaccgaacaa ggcgatcgaa gcgacgatct
     3901 cgacgatctg gccgtcgtcg aagtgctcct tcagcgcgtc gaagtcggcc tgcgtggctt
     3961 cgttcggttg cttcgcggct ttcagcgcca gccggacggc ggcgcgctcg gccggggtga
     4021 ataactcgct ttcctcgaag ttccacagcg cggcaatctt ctcgtcggac gccttgtaga
     4081 tgctcgacag gttggccatg tgcgactggc agtagaggca gccggaggcg tagctgctgg
     4141 ccaggctgac cagcatcttg gtttcgtggg gcacggtgcc ctcgtacagc accgcctggt
     4201 tcaacgccat gaaggcgcgc act
//
LOCUS       DAOQPA010000198         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_13777_length_4211_cov_19.147016, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000198 DAOQPA010000000
VERSION     DAOQPA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13777_length_4211_cov_19.147016"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKX01_16655"
     CDS             <1..629
                     /locus_tag="PKX01_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMZ77818.1"
                     /translation="DMAMMRHCRQCRADAVGLLGEDRGAEFTLDKIDVMDVDYEAAMA
                     RRKEVHDAIIDQLEAKRGKKAPAADSDIPADARPVLMAVAGKNGVVAEHFGHAREFLI
                     YEASPAGVRFISHRKTELYCGGADTCGEGDEDPESLLARNIKVLEGCEVVLCSKIGYE
                     PWGMLEAAGIAPNGEHAMEPIEDAVMAVYKELAIAGRLSRPAPSKMVA"
     gene            690..968
                     /locus_tag="PKX01_16660"
     CDS             690..968
                     /locus_tag="PKX01_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ77819.1"
                     /translation="MALQITNNCVNCWACVDVCPNDAIYLDSPHFLIDDMKCTECLGD
                     HADPQCATICPIECAIVNELDEALNPLGSLTGIPMEKFIEMGVIKEAA"
     gene            968..1444
                     /locus_tag="PKX01_16665"
     CDS             968..1444
                     /locus_tag="PKX01_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HMZ77820.1"
                     /translation="MALVTFFEKPGCQGNARQKALLTAAGHIVEAHSLKAEPWTRERL
                     LQFLGGLPVADWFNRSAPAVKAGEIVPETLDADAAMALLLANPLLIRRPLMEVDRSRM
                     VGFDTAVVDAWIGLGEAERPAGNIEACVHGPAGHGRCGSAAADAEEEGHHGPCGTH"
     gene            1422..2693
                     /locus_tag="PKX01_16670"
     CDS             1422..2693
                     /locus_tag="PKX01_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HMZ77821.1"
                     /translation="MAPVGLTESAVELAPGVHWVGAFDPHLRSFDIILKTANGTSYNA
                     YVVRGEKGVAVIDTVKEGFADTFFQRLESVARYDEITTIVLNHLEPDHSGALPELLRR
                     APQARVYLSVRAQMMLKALLKPTGEKAPDYTPVTTDDTVDLGGRTLRFLHTPYLHWPD
                     TQCTYLVEEAMLFSGDVFGCHFCDPRLFNDCVGDFRFSFEYYYAHIMRPFRENVLDAV
                     ALIEPLDLKLIAPAHGPVLRHAPRDYVHRYRELATPRLFNEARSEKTLVIFYLSAYGN
                     TRRMAECIAQGAERIRGVRVSLYDLEGGESDIFTDLVEEADGLAFGSPTINADAVKPI
                     WDLLSSLTTVNVKGKLGAAFGSYGWSGEAVRLIEDRLRGLKLRVPVEGLRIKLVPDEA
                     ELAQCCTLGESLARHLTGQLEHRELDMASLA"
     gene            2719..3006
                     /locus_tag="PKX01_16675"
     CDS             2719..3006
                     /locus_tag="PKX01_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ77822.1"
                     /translation="MPKAKVTFEDIGVSVTVPAGTRLIEVSEKVGAGITYGCREGECC
                     TCLTKIVSGADNLAPPSILEDQVLKDNMAPRQHRLACQAQIIGGEIVVRPA"
     gene            3136..3498
                     /locus_tag="PKX01_16680"
     CDS             3136..3498
                     /locus_tag="PKX01_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ77823.1"
                     /translation="MALITFSSPMHKDKTVYAVAGSHTQTILSLAKEHHIPIDFGCQE
                     GNCGTCLVKVSSVDGKRRPMGGPLNVREVAALMDMGHITKAQVDQMYVDDIPPTQWRL
                     ACQMIVRDEDILVEYPSK"
     gene            3509..4096
                     /locus_tag="PKX01_16685"
     CDS             3509..4096
                     /locus_tag="PKX01_16685"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HMZ77824.1"
                     /translation="MNGPDLLSLRQRLHTRLLELPVTARVAGDPNRSLFASLLAGQSA
                     GFGCLPAGLGLLPAQHTTLLEEFFPDALDASVLLVPLQRPVEAIPEWDDLYKLLTDHR
                     AGRHDAELWAARVIATACAGRDHLWQDLGFNNRTELSALITRNFPDLAAANSGDMKWK
                     KFLYRQFCAIEGIYVCPAPSCGVCADHAKCFGPEE"
BASE COUNT          745 a         1548 c         1278 g          640 t
ORIGIN      
        1 gcgacatggc gatgatgcgc cactgccgcc agtgccgcgc cgacgcggtg ggcctgctgg
       61 gcgaagaccg gggcgccgag ttcaccctgg acaagatcga cgtcatggat gtggattacg
      121 aggccgccat ggcccgccgc aaggaagtcc acgacgccat catcgaccag ctcgaagcca
      181 agcgcggcaa gaaggcgccg gccgccgaca gcgacatccc ggccgacgcc cgcccggtgc
      241 tgatggccgt ggccggcaag aacggcgtgg tggccgagca cttcgggcac gcccgcgagt
      301 tcctcatcta cgaagcctcg cccgccggcg tgcgcttcat cagccaccgc aagaccgagc
      361 tgtactgcgg cggcgccgac acctgcggtg aaggtgatga ggatcccgag tccctgctcg
      421 cccgcaacat caaggtgctc gaaggctgcg aggtggtgct gtgctccaag atcggctacg
      481 agccgtgggg catgctggaa gccgccggca tcgcccccaa cggcgagcac gccatggagc
      541 ccatcgagga cgcggtgatg gcggtgtaca aggaactggc catcgccggc cgcctcagcc
      601 gccccgcccc gtcgaagatg gtggcctgac ctccctgccc tcgtgatctg ccgccccctg
      661 cggggggcgg cccacccgga gattcgaaca tggcactgca aatcaccaac aactgcgtca
      721 attgctgggc ctgcgtggac gtgtgcccca acgacgcaat ctacctggat tcgccccact
      781 tcctgatcga cgacatgaag tgcaccgagt gcctgggcga tcacgccgat ccccagtgcg
      841 cgacgatctg cccgatcgag tgcgccatcg tcaatgagct ggacgaggcg ctcaacccgc
      901 tgggctccct caccggcatc ccgatggaga agttcatcga gatgggcgtg atcaaggaag
      961 ccgcctgatg gccctcgtca ccttcttcga gaagcccggc tgccagggca acgcgcgcca
     1021 gaaggcgctg ctcacggccg ccgggcatat cgtggaggcg cacagcctca aggccgagcc
     1081 gtggacgcgg gagcgcctgc tccagttcct cggcggcctg cccgtggccg actggttcaa
     1141 tcgttccgcc ccggccgtga aggccgggga gatcgtcccg gaaacgctgg acgcggacgc
     1201 cgcgatggcc ctgctcctgg ccaaccccct gctgatccgc cgcccgctga tggaggtgga
     1261 ccgcagccgg atggtcggct tcgacaccgc cgtggtagac gcctggatcg gtctgggcga
     1321 ggcggagcgc cccgccggca acatcgaagc ctgcgtgcat ggcccggcgg gccacggccg
     1381 ctgtggcagc gctgccgcgg acgcagagga agaagggcac catggcccct gtgggactca
     1441 ctgaatccgc cgtcgaactg gctccgggcg tccactgggt gggcgccttc gacccgcatc
     1501 tgcggagctt cgacatcatc ctcaagaccg ccaacggcac cagctacaac gcctacgtgg
     1561 tgcgcggcga gaagggggtg gcggtcatcg acacggtgaa ggaaggcttc gctgacacct
     1621 tcttccagcg cctggaatcc gtcgcccgtt acgacgagat caccaccatc gtcctcaacc
     1681 acctggaacc ggaccacagc ggcgccctgc ccgagctgct gcggcgcgca ccccaggcgc
     1741 gggtgtacct gtcggtacgc gcccagatga tgctcaaggc cctgctcaag cccaccggcg
     1801 agaaggcccc ggactacacc ccggtcacca ccgacgacac ggtggacctg ggcggccgca
     1861 ccctgcgctt tctgcacacg ccctacctgc actggcccga cacccagtgc acctacctgg
     1921 tcgaagaggc gatgctgttc tccggcgacg tgttcggctg ccacttctgc gacccgcgcc
     1981 tgttcaacga ctgcgtgggc gacttccgct tctccttcga gtactactac gcccacatca
     2041 tgcggccgtt ccgtgagaac gtccttgacg cagtggcgct gatcgaaccc ctcgacctga
     2101 agcttatcgc ccccgcccac ggcccggtgc tgcgccatgc gccgcgggac tacgtgcacc
     2161 gctaccggga gctcgccact ccgcgcctct tcaacgaggc ccgcagcgaa aagaccctgg
     2221 tgatcttcta cctctcggcc tacggcaaca cccggcgcat ggccgagtgc atcgcccagg
     2281 gcgccgagcg aatccgcggc gtgcgcgtct ccctctacga cctggaaggc ggcgagtcgg
     2341 acatcttcac cgatctggtg gaggaagccg acggcctcgc cttcggcagc cccaccatca
     2401 acgcggacgc ggtcaagccc atctgggacc tgctctcctc cctcaccacc gtcaacgtca
     2461 agggcaagct cggcgcggcc ttcggctcct acggctggag cggcgaggcg gtgcgcctga
     2521 tcgaggaccg cctgcgcggc ctcaagctgc gggtgccggt ggagggcctg cgcatcaagc
     2581 tggtgcccga cgaagcggaa ctcgcgcaat gctgcaccct cggcgaaagc ctggcccgtc
     2641 atctcaccgg ccagctggaa caccgggaac tggatatggc gagcctcgcc tgatattgcc
     2701 ctctcaagga agcgaaaaat gcccaaagcc aaagtcacct tcgaagacat cggtgtctcc
     2761 gtcaccgtcc cggccggtac ccggctcatc gaagtctcgg aaaaagtcgg cgccggcatc
     2821 acctacggct gccgcgaagg cgaatgctgc acctgcctga ccaagatcgt ctccggcgcc
     2881 gacaacctcg ccccgcccag catcctggaa gaccaggtgc tcaaggacaa catggccccg
     2941 cgtcagcacc ggctggcctg ccaggcccag atcatcggcg gagagatcgt cgtccgcccc
     3001 gcctgatcgc ggccacaccc gacacaacaa gcccagcggg aactgccgga cgcggtccgg
     3061 ccttccccgc cctacggggc aggcgccccc ccaaagagaa tatcgcctat caaccgttcg
     3121 tcaggagaac ccaccatggc ccttatcacc ttcagcagtc cgatgcacaa ggacaagacc
     3181 gtgtatgccg tcgccggcag ccacacccag accatccttt cccttgccaa ggaacaccac
     3241 atccccatcg acttcggctg ccaggaaggc aactgcggca cctgtctcgt caaggtctcc
     3301 tccgtggatg gcaagcgccg tcccatgggc ggccccctga acgtccgcga agtggccgcc
     3361 ctgatggaca tgggccacat caccaaggcc caggtggacc agatgtacgt ggatgacatc
     3421 ccccccaccc agtggcgtct cgcctgccag atgatcgtcc gcgacgaaga catcctggtg
     3481 gaatacccct ccaagtgaga cccctgccat gaacggtccg gatctcctca gcctccgcca
     3541 gcggctgcac acgcggctgc tggaactgcc ggtgacggcc agggtggccg gcgatccgaa
     3601 ccgttcattg tttgccagcc tgctcgccgg gcagagtgcc ggtttcggct gtctgcccgc
     3661 cgggctgggc ctgctccccg cccagcacac cacactgctg gaagaattct tcccggacgc
     3721 cctcgacgcc agcgtcctcc tcgtgcccct gcagcgcccg gtggaggcca tccccgagtg
     3781 ggacgacctc tacaagctcc tcaccgacca ccgcgccggc cgccacgacg ccgagctgtg
     3841 ggccgcccgc gtcatcgcca ccgcctgcgc cggccgcgac cacctgtggc aggacctggg
     3901 cttcaataac cgcaccgagc tgtccgccct gatcacccgc aacttccccg acctggccgc
     3961 cgccaacagc ggcgacatga agtggaagaa gttcctctac cgtcagttct gcgccatcga
     4021 aggcatctac gtctgcccgg cgccgtcctg cggcgtctgc gccgaccacg ccaagtgctt
     4081 cggaccagag gagtgagcgg aacgctccgt cctctatcct cgctgagctg cgacgtcatc
     4141 cctcggcccc gccccatgga aatgccccgg aaccattttt tgatgggatg ggcggatttt
     4201 taaatgggtc c
//
LOCUS       DAOQPA010000199         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_13952_length_4180_cov_17.513697, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000199 DAOQPA010000000
VERSION     DAOQPA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13952_length_4180_cov_17.513697"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="PKX01_16690"
     CDS             <1..2397
                     /locus_tag="PKX01_16690"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ77825.1"
                     /translation="MNEAETRAELIDPLLAAAGWGVLEGSRIRREYPITLGRLEGAGR
                     RGKALSADYVLEYRNTKLAVVEAKSDEKALTEGVGQAKDYAGKLAIRFTYASNGKGIY
                     GIDMQTGVEGELPAFPGPEALWLATFATENTWRDRFSAVPFEDRGGYFQGRYYQDIAI
                     ERVLAALAEGRDRVLLTLATGTGKTFIAFQLAWKLFHSRWNLGDWKKTGEPTRRPRVL
                     FLADRNTLADQAFNAFSAFPDDALVRIAPDDIKKKGKVPKNGSIFFTIFQTFMSGPPK
                     DGEPSPYFGEYPPDFFDFIVIDECHRGGANDESTWRGILEYFAPAVQLGLTATPKRQE
                     NADTYAYFGEPVFIYSLKDGINDGFLTPFRVKQIATTLDDYVFTPDDTLLEGEIEAGK
                     RYEEADFNRRIEIVAREKKRVEIFMAEIDQKEKTLVFCATQAHALLVRDLINQVKTSR
                     DPNYCQRVTAEDGKLGDEALRAFQDNEKSIPTILTTSQKLSTGVDARNVRNIVLMRPV
                     NSMIEFKQIIGRGTRLYDGKDYFTIYDFVRAHHHFSDPEWDGEPLSPATTEVCAVCQR
                     VPCVCAREPSPPCYICGNRPCICPRTRCPRCGNQPCTCRQKAKIKLADGKERQIQHMS
                     ATSFWHPDGTPMSAAQFMEALFGQLPEFFRDEDELRTIWSAPDTRRKLLAGMAEKGFG
                     KDQLAEMQTIIEAENSDLFDVLAYVAYAEAPLTRNERAERARAVITTRFNDRQQAFLA
                     FVLAHYVTVGVEELDEAKLGPLLKLRYNNAIADALADLGTPDAVRNAFVGFQQYLYLR
                     "
     gene            complement(2519..3427)
                     /gene="hemF"
                     /locus_tag="PKX01_16695"
     CDS             complement(2519..3427)
                     /gene="hemF"
                     /locus_tag="PKX01_16695"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HMZ77826.1"
                     /translation="MPDRHAVKAYLLDLQARIVAGLEAHDGRPFLTDAWTRPAGGGGV
                     TRLIEEGDFFERGGCNFSHVMGEGLPPSATAARPELAGRRYEAMGVSLVLHPRNPYAP
                     TVHMNVRFFIASADGKDDVWWFGGGMDLTPYYPFEEDVRHFHATCKAAVLPWGEAEYQ
                     RLKDWCDRYFFLKHRNEPRGVGGLFFDDLGADGHTDFDSAFALTRSVGDAFLPAYLPL
                     AQRRRDTPYGERERDFQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPIVKW
                     RYDWHPEAGSAEEKLYTEFLVAREWA"
     gene            complement(3489..3854)
                     /locus_tag="PKX01_16700"
     CDS             complement(3489..3854)
                     /locus_tag="PKX01_16700"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HMZ77827.1"
                     /translation="MPHLRIEYSANVTDRFDPQRLVDAACEILVASGVFSPPDSIKVR
                     LIPVEHFRTGSGADQGFIHAGLALLSGRDTETKRALTGALVESITLLVQPGPRPVQIT
                     AEARDMDRSTYSKNLAPAS"
     gene            complement(3866..>4180)
                     /locus_tag="PKX01_16705"
     CDS             complement(3866..>4180)
                     /locus_tag="PKX01_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HMZ77828.1"
                     /translation="AEWDRRFALGVVMAAAGYPDSPRKGDALHGLPTAQGDDAHVFHA
                     GTTERDGQVVTSGGRVLCVTALGDNVRSAQKRAYELVDQIHFDGMQCRRDIGYRAVAR
                     KG"
BASE COUNT          730 a         1216 c         1515 g          719 t
ORIGIN      
        1 atgaacgaag ccgaaacccg tgccgaactg atcgaccccc tgctggcggc agcgggttgg
       61 ggcgtgctgg aaggcagccg catccgccgc gagtacccga tcaccctggg gcggcttgaa
      121 ggcgcggggc ggcgcggcaa ggccttgtcg gcggactacg tgctggaata ccgcaacacc
      181 aagcttgccg tggtcgaggc caagtcggat gaaaaggcgc tgaccgaggg cgtggggcag
      241 gccaaggatt acgcgggcaa gctggcgatc cgcttcacct acgcgagcaa cggcaagggc
      301 atctacggca tcgacatgca gaccggtgtg gagggcgagt tgccggcctt tcccggcccg
      361 gaggcgctct ggctggccac ctttgcgaca gagaacacat ggcgtgaccg cttttcggcc
      421 gtgcccttcg aggatcgggg cggctacttt cagggtcgct actatcagga catcgccatc
      481 gagcgcgtgc tggcagcgct ggccgagggg cgtgatcgcg tcctgctgac cctggccacg
      541 ggcacgggca agaccttcat tgcctttcag ctggcgtgga agctgtttca cagccgctgg
      601 aatctgggtg actggaagaa gaccggcgag ccgacgcggc gcccgcgcgt cctgtttctg
      661 gccgaccgca acacgctggc cgatcaggct ttcaacgcct tctcggcctt tccggacgat
      721 gcgctggtgc gcattgcgcc cgacgacatc aaaaagaagg gcaaggtgcc gaagaacggc
      781 tcgatcttct tcaccatctt tcagactttc atgagcggcc ctccgaagga tggagagcct
      841 tccccctact ttggcgaata cccgccggat ttcttcgact tcatcgtgat cgacgaatgc
      901 caccggggcg gagccaacga tgaaagcacc tggcgcggca tccttgaata ctttgcaccg
      961 gccgtgcaac tgggcctcac cgccacgccg aagcggcagg agaacgccga cacctacgcc
     1021 tatttcggcg agcccgtctt catctattcg ctcaaggacg gcatcaacga cggctttctg
     1081 acgcccttcc gcgtgaagca gattgcgacg accctcgacg attacgtctt taccccggac
     1141 gatacgctgc tggagggcga gatcgaggcc ggcaagcgct atgaggaggc ggatttcaac
     1201 cgccgtatcg agatcgtcgc ccgcgagaag aagcgcgtgg agatcttcat ggccgagatc
     1261 gaccagaagg agaagacgct tgtcttctgc gccacccagg cccacgcact gctggtgcga
     1321 gatctgatca atcaggtgaa gacgagccgc gatcccaact actgccagcg ggtgacggcc
     1381 gaggatggca agctgggtga tgaggcgctc cgcgcctttc aggacaacga gaagagcatt
     1441 ccgaccatcc tgaccacctc gcagaagctg tccaccgggg tagatgcgcg caatgtgcgc
     1501 aacatcgtgc tgatgcgccc ggtcaattcg atgatcgagt tcaagcagat catcgggcgt
     1561 ggcacccggc tctacgacgg caaggactat ttcaccattt atgatttcgt gcgggctcac
     1621 catcatttca gcgatccgga gtgggatggt gagcctctgt cacctgccac cacggaggtc
     1681 tgcgcggttt gccagcgtgt gccgtgtgtg tgtgccaggg agccgtcacc gccgtgctat
     1741 atctgcggaa atcggccgtg tatctgtcct agaacacgct gcccaaggtg tggaaaccag
     1801 ccgtgtacat gcaggcagaa agccaagatc aagttggccg acggcaagga gcgtcagatc
     1861 cagcacatga gtgcgaccag tttctggcat ccggacggta ctccgatgtc ggcggcgcag
     1921 ttcatggaag ccctgttcgg gcagctaccg gagtttttcc gggacgaaga tgaattgcgc
     1981 acgatctgga gtgcaccgga cacccgccgg aaattgctgg caggaatggc cgaaaagggg
     2041 ttcggcaagg atcagctcgc cgagatgcag acgatcatag aggcggaaaa cagcgatctg
     2101 ttcgatgtgt tggcctacgt ggcctacgcg gaagcgccgc tgacccggaa cgaacgggcc
     2161 gagcgggcgc gggccgtgat caccacacgc ttcaacgaca ggcagcaggc ctttctggcc
     2221 ttcgtgctgg cgcactacgt gacggtaggt gtggaagagc tcgacgaagc caaactcggg
     2281 ccactattga agctccggta caacaatgcg attgccgatg ccttggcaga tctgggcacg
     2341 cccgacgccg tccggaatgc atttgtcggt tttcagcaat acctctacct gcgctagcac
     2401 tttcgtcggc tgcctgcctg cgtgttcagg aagccggctg agtgcgtgaa accttgtcgc
     2461 aggggcgact acggtattgg cgtatcaacg tcccgtatcc gaatcgaggg gcagcccctc
     2521 acgcccattc ccgcgccacc agaaactccg tgtatagctt ttcctccgcg ctgcccgctt
     2581 ccgggtgcca gtcgtagcgc catttgacga tggggggcat ggacatgagg atggattcgg
     2641 tgcggccgcc ggactggagg ccgaagaggg tgccgcggtc gaagaccagg ttgaattcca
     2701 cgtagcggcc gcggcggtag gcctggaagt cgcgctcgcg ttcgccgtag ggggtgtcgc
     2761 ggcggcgctg ggcgaggggg aggtaggcgg gcaggaaggc gtcgccgacg ctgcgggtga
     2821 gggcgaaggc gctgtcgaag tcggtgtggc cgtcggcgcc caggtcgtcg aagaagaggc
     2881 cgcccacgcc gcggggctcg ttgcggtgct tgaggaagaa gtagcggtcg caccagtcct
     2941 tgaggcgctg gtattcggct tcgccccagg gcagcacggc ggccttgcag gtggcgtgga
     3001 agtggcggac gtcttcttcg aaggggtagt agggggtgag gtccatgccg ccgccaaacc
     3061 accacacgtc gtccttgccg tcggcgctgg cgatgaagaa gcgcacgttc atgtggacgg
     3121 tgggggcgta ggggttgcgg gggtggagga cgagggagac gcccatggct tcgtagcggc
     3181 ggccggcgag ctcggggcgg gcggcggtgg cggagggggg caggccttcg cccatgacgt
     3241 gggagaagtt gcagccgccg cgctcgaaga agtcgccctc ttcgatgagg cgggtgacgc
     3301 cgccgccgcc ggcggggcgg gtccaggcgt cggtgaggaa ggggcggccg tcgtgggctt
     3361 cgaggccggc gacgatgcgt gcctggaggt cgaggaggta ggctttgacg gcgtggcgat
     3421 ccggcatggg gggctctcgg gcggggaagg gcgaatgaaa aaccgccggc agggggccgg
     3481 cggtgggatc agctggcggg ggccaggttc ttggagtagg tgctgcggtc catgtcgcgg
     3541 gcttcggcgg tgatctgcac gggccgcggg ccgggctgga cgagcagggt gatgctttcg
     3601 acgagggcgc cggtgagggc gcgcttggtc tcggtgtcgc ggccggagag cagggccagg
     3661 ccggcgtgga tgaagccctg gtcggcgccg ctgccggtgc ggaagtgctc gacggggatc
     3721 agccgcacct tgatggaatc cggcgggctg aagacgccgc tggcgacgag gatttcgcag
     3781 gcggcgtcca ccaggcgttg gggatcgaag cggtcggtca cgttggcgct gtattcgatg
     3841 cgcaggtggg gcatggcgcg gtgcctcagc ccttgcgcgc gacggcgcgg tagccgatgt
     3901 cgcggcggca ctgcatgccg tcgaagtgga tctggtccac gagttcgtag gcgcgcttct
     3961 gggcgctgcg cacgttgtcg ccgagggcgg tgacgcacag cacgcggccg ccgctggtga
     4021 cgacctggcc gtcccgctcg gtggtgccgg cgtggaagac gtgggcgtcg tcgccctggg
     4081 cagtgggcag gccgtggagg gcgtcgccct tgcgggggct gtcggggtag ccggcggcgg
     4141 ccatcaccac gccgagggcg aagcggcggt cccattcggc
//
LOCUS       DAOQPA010000200         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_14039_length_4164_cov_17.144317, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000200 DAOQPA010000000
VERSION     DAOQPA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14039_length_4164_cov_17.144317"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..2129)
                     /gene="ftsH"
                     /locus_tag="PKX01_16710"
     CDS             complement(315..2129)
                     /gene="ftsH"
                     /locus_tag="PKX01_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMZ77829.1"
                     /translation="MEKKTQFSLWYFLFALFAVFTLHDLWVQVRTTEPLPYSEFQRRL
                     KAGEVAEIAIADNTIQGSFKQALPDGRQKFVTTRVDPELARDLGQYDVKFTGVVESTL
                     LRDILGWVLPAVIFVAIWLFAMRRFGGKQGFGGGFMNLGKSKAKVYVEANTGVSFADV
                     AGVDEAKDELKEVVGFLKDPKSYGRLGGRVPKGVLLVGPPGTGKTLLAKAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARAKAPAIIFIDELDAMGRARGAFAMGGHDE
                     KEQTLNQLLVELDGFDSSSGLVLLAATNRPEVLDAALLRAGRFDRQVLVDRPDKKGRI
                     AILQVHMKKITLAPDLDAETVAALTPGFSGAELANLCNEAALVATRRNGSAVTVDDFT
                     VAVERIVAGLEKKNRVLNEHERTVVAHHEMGHALVAMALPGTDPVHKISIIPRGVGAL
                     GYTIQRPTEDRFLMTRAELENKMAVLLGGRAAEQIIFGEVSTGAADDLQKVTSIARSI
                     VMRFGMDDQLGNVAYDTERNTFLGQAAAAPAERSYSEETAREIDCAVRHIVARAFERS
                     TAILSAHKDLLERSAADLLQRETFDEADLKALHTRVAA"
     gene            complement(2287..3075)
                     /gene="lgt"
                     /locus_tag="PKX01_16715"
     CDS             complement(2287..3075)
                     /gene="lgt"
                     /locus_tag="PKX01_16715"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ77830.1"
                     /translation="MLVHPQFNPVALSIGPLDVRWYGLMYLTAFILFVALGRAHARRR
                     PELGWDAQQIDDLLFYGVLGVILGGRLGYILFYKPAYYFSHPVEMFAVWQGGMAFHGG
                     FLGVLIAVWLFARSHDRPWLAVTDFIAPLVPLGLAAGRLGNFINGELVGRVTDVPWAM
                     IFPQVDMQPRHPSQLYQFTLEGVCLFVLLWVFSAKPRPRGLVSAVFLIGYGGFRILAE
                     FGREPDDFLGLLALNLSMGQWLSLPMIVIGIVMAVIAVRRAADT"
     gene            complement(3103..3786)
                     /locus_tag="PKX01_16720"
     CDS             complement(3103..3786)
                     /locus_tag="PKX01_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HMZ77831.1"
                     /translation="MTDTPATAENPAFDLDDFEVRVLAVLIEKSFVTPDIYPLSLNAL
                     LAGCNQLTGREPVMSLSETAVQDALDRLAERGLVSQRHQAGARVAKWEHQIRLRHSLT
                     PPMQAVLALLMLRGPQTPAEIRARCDRLHAFTSTAEVEEILEKLGDKFPPMVVQLPKA
                     PGTKEARFAHLLAGEGAVAALGHTTEAGDAPSPRSGRVAELEDEVRRLRQQVDWLTAE
                     FEAFRKQFQ"
     gene            complement(3881..>4164)
                     /locus_tag="PKX01_16725"
     CDS             complement(3881..>4164)
                     /locus_tag="PKX01_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HMZ77832.1"
                     /translation="SFAEAADIAADIARSPDDCRAALRHKGHGLIDDVAAEVALWPAF
                     HSAPPVRGPALPLGLVREPFVRDGVKVGRNDPCPCGSGRKYKKCHGAAV"
BASE COUNT          643 a         1253 c         1622 g          646 t
ORIGIN      
        1 tgatcttggc gagggtgggc acttcctggg cggcggcggg ctgggccagg gcggcgagca
       61 ggccagcggc ggaggccaga gtgacgagag gcaggcggag gcggaacatg ggaggactct
      121 ccttgcaaag gtggttgtcc gaactcgggg tgcgtccgcc tgcaggggcg ccggagcgag
      181 gcggcacacg gttcttcggg ctggatggaa cagcaaggac gattgtagcc ctgccatcgc
      241 cgcgcgggcg agcagtgcag ggaccgcgac gcggacgccc gcgctgaggc gggcgcggcg
      301 cggtcggggt ggcgtcaggc ggcgacgcgg gtgtggaggg ccttgaggtc ggcttcgtcg
      361 aaggtttccc gctggagcag gtcggcggcg ctgcgctcca gcagatcctt gtgggcgctg
      421 agaatcgccg tgctgcgctc gaaggcgcgg gcgacgatgt ggcggaccgc gcagtcgatc
      481 tcgcgggcgg tttcctccga atagctgcgt tcggccggcg cggccgccgc ctgaccgagg
      541 aaggtgttgc gctcggtgtc gtaggccacg ttgccgagct ggtcgtccat gccgaagcgc
      601 atgacgatgg agcgggcgat ggaggtgacc ttctgcaggt cgtcggcagc gcccgtggag
      661 acttcgccga agatgatctg ttctgcggcg cgcccgccca gcagcacggc catcttgttc
      721 tccagttcgg cgcgggtcat caggaagcgg tcttcggtgg gacgctggat ggtgtagccc
      781 agggcgccga cgccccgcgg gatgatgctg atcttgtgca ccgggtcggt gccgggcagg
      841 gccatggcga ccagggcgtg gcccatctcg tggtgggcga cgacggtgcg ctcgtgttcg
      901 ttgaggacac ggttcttctt ctcgaggccg gcgacgatgc gctccaccgc cacggtgaag
      961 tcgtcgacgg tgacggcgct gccgttgcgc cgggtggcga cgagggcggc ctcgttgcac
     1021 aggttggcca gttcggcgcc ggagaagccg ggcgtcagcg ccgcgacggt ctccgcgtcc
     1081 agatccgggg ccagggtgat cttcttcatg tgcacctgca ggatggcgat gcggcccttc
     1141 ttgtccgggc ggtccaccag cacctgacgg tcgaagcggc cggcccgcag cagggcggcg
     1201 tcgaggactt ccggccggtt ggtggcggcc agcaggacca ggccggagga ggagtcgaag
     1261 ccatccagtt cgacaagcag ctggttgagg gtctgttcct tctcgtcgtg gccgcccatg
     1321 gcgaaggcac cgcgggcgcg gcccatggcg tccagttcgt cgatgaagat gatggcgggc
     1381 gccttggcgc gggcctgctc gaagaggtcg cggacgcggg cggcccccac gccgacgaac
     1441 atctcgacga actcggagcc ggagatcgag aagaagggca cgcccgcctc gccggcgacg
     1501 gccttggcga gcagggtctt gccggtgccc ggcgggccga ccagcagcac gcccttgggt
     1561 acgcgtccgc ccaggcggcc gtaggatttg gggtccttga ggaagccgac gacttccttg
     1621 agctcgtcct tggcctcgtc cacgccggcc acgtcggcaa agctcacgcc ggtattggcc
     1681 tccacgtaga ccttggcttt gctcttgccc aggttcatga agccgccgcc gaagccctgc
     1741 ttgccgccga agcggcgcat ggcaaagagc cagatggcga cgaagatgac cgcgggcagc
     1801 acccagccga ggatgtcccg cagcagggtg ctctccacga cgccggtgaa tttgacgtcg
     1861 tactggccca gatccctggc cagttcagga tcgacgcggg tggtgacgaa cttctggcgg
     1921 ccatcgggca gggcctgctt gaagctgccc tggatggtgt tgtcggcgat ggcgatctcg
     1981 gccacctcgc cggccttcag cctgcgctgg aattcactgt agggcagcgg ctcggtggtg
     2041 cgtacctgga cccacaggtc gtgcagggtg aacactgcga acagagcgaa gaggaagtac
     2101 cacaggctga actgggtttt cttttccatg gcgggcggaa acaagggagg tttccggcag
     2161 atgggggcgt ctgccgcagg attcaagcgt ccggggcgaa cgcctcccgg tgttcgccgt
     2221 gggggcgggt gtgtgcggca cgtggagggg cagtccggcc gcgggcgcgg ccgggccgtg
     2281 gggcgatcag gtgtcggccg cccggcggac ggcaatcacc gccatgacga tgccgatcac
     2341 gatcatcggc agggacagcc actgtcccat ggacaggttg agggcgagca ggccgaggaa
     2401 gtcgtcgggc tcccggccga attccgccag gattcggaaa ccgccgtagc cgatgaggaa
     2461 caccgccgag accagacccc gcggccgcgg tttggcggag aagacccaca acagcacgaa
     2521 caggcacaca ccttcgaggg tgaactggta gagctgggac gggtggcgcg gttgcatgtc
     2581 cacctgcggg aagatcatcg cccagggcac gtctgtgaca cggccgacca gttcgccgtt
     2641 gatgaagttg cccaggcgtc ctgccgccag gccgaggggg acgagcgggg cgatgaagtc
     2701 cgtgaccgcc agccatgggc ggtcgtgact gcgggcgaac agccagacgg cgatgagcac
     2761 gccgaggaag ccgccgtgga aagccatgcc gccctgccag acggcgaaca tctcgacggg
     2821 atgcgagaag tagtacgccg gtttgtagaa caggatgtat ccgagccgcc cgccgaggat
     2881 cacgccgagc accccgtaga acagcaggtc gtcgatctgc tgggcgtccc agccgagttc
     2941 cggccggcgc cgggcatggg cacggccaag ggccacgaag aggatgaagg cggtgaggta
     3001 catgaggccg taccagcgca cgtcgagggg accaatggag agcgcgaccg ggttgaactg
     3061 gggatgaacg agcatgagtg cctcggggaa tgcgggacgg acttactgga actgcttgcg
     3121 gaaggcttcg aattcggcgg tcagccagtc cacctgctgg cgcagacggc ggacttcgtc
     3181 ttccagttcg gccacccggc cgctccgcgg cgagggcgca tcgccggcct ccgtggtgtg
     3241 accgagggcg gccacggccc cttcgccggc cagcaggtgg gcgaagcggg cttccttggt
     3301 gcccggcgcc ttgggcaact ggacgaccat gggcgggaac ttgtcgccga gcttttcgag
     3361 gatctcctcg acctccgcgg tggacgtgaa ggcgtgcagg cggtcgcagc gggcgcggat
     3421 ctcggccggc gtctgggggc cgcgcagcat cagcagggcc agcaccgcct gcatgggcgg
     3481 ggtgagggaa tggcgcaggc ggatctggtg ttcccacttg gccacccgcg cgccggcctg
     3541 gtggcgctgg ctgacgagtc cccgctcggc aaggcggtcg agggcgtcct gcacggcggt
     3601 ctcggacagg ctcatcaccg gctcgcggcc ggtgagctgg ttgcagccgg cgaggagggc
     3661 gttgagggac aggggataga tgtcgggcgt gacgaaggat ttctcgatga ggacggcgag
     3721 gacgcggact tcaaagtcgt ccaggtcgaa ggccgggttc tcggcggttg cgggcgtgtc
     3781 ggtcatgggc ggtttggcag gaggcgttgg gtcgaaggct agcggagtcc gtggggcgtc
     3841 cggatggcgg caggattgcg ggggtgttgc agccggcggc tcagaccgcg gcgccgtggc
     3901 acttcttgta tttgcgaccg ctgccgcagg ggcaggggtc gttgcgtccc accttgacgc
     3961 cgtcgcgcac gaaaggttcg cgcaccaggc cgaggggcag ggccggcccg cgtaccggcg
     4021 gggcgctgtg gaaggcgggc cacagggcga cttctgcggc cacgtcgtca atcaggccgt
     4081 ggcccttgtg gcgcagggcg gcgcggcagt cgtccgggct gcgggcgatg tcggcggcga
     4141 tgtcggcggc ctcggcgaag ctgc
//
LOCUS       DAOQPA010000201         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_14517_length_4078_cov_22.405667, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000201 DAOQPA010000000
VERSION     DAOQPA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_4078_cov_22.405667"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKX01_16730"
     CDS             complement(<1..404)
                     /locus_tag="PKX01_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77833.1"
                     /translation="MAYSYTEKKRIRKSFAKRVAVKNVPYLLATQLESYASFLQAETP
                     PAARATQGLQAAFNSIFPIASHSGNARLEFVQYHLGEPAFDVKECQQRGLTFASPLRA
                     RVRLVILDKEAAKETIKEVKEQEVYMGEIPLMT"
     gene            complement(602..979)
                     /gene="rplL"
                     /locus_tag="PKX01_16735"
     CDS             complement(602..979)
                     /gene="rplL"
                     /locus_tag="PKX01_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMZ77834.1"
                     /translation="MAITKEDILEAVGAMTVMELNDLVKAFEEKFGVSAAAMAVAAPG
                     AGGAAAAAVEEQTEFTVMLLAAGDKKVEVIKVVRAVTGLGLKEAKDLVDGAPKPVKEG
                     IAKADADALKKQLEDAGAKVEVK"
     gene            complement(1030..1530)
                     /gene="rplJ"
                     /locus_tag="PKX01_16740"
     CDS             complement(1030..1530)
                     /gene="rplJ"
                     /locus_tag="PKX01_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMZ77835.1"
                     /translation="MSLNLDDKKAVVAEVSAQVANAQTIVVAQYAGIEVGDLTALRAK
                     ARASGVYLRVLKNTLARRAVADTPFAPLAEQLTGPLIYSISADPVAAAKVLADFSKTN
                     DKLVLKAGCYAGKLLDKEGVQALASIPSREELLAKLLGVMQAPVSGFAGLLAALAKKR
                     EEGAAA"
     gene            complement(1756..2457)
                     /gene="rplA"
                     /locus_tag="PKX01_16745"
     CDS             complement(1756..2457)
                     /gene="rplA"
                     /locus_tag="PKX01_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMZ77836.1"
                     /translation="MAKLSKRVQALRAKVDRNKPYPLTEALGLVKECATAKFDESIDI
                     AVNLGVDAKKSDQIVRGSVVLPAGTGKSVRVAVFAQGEKAEAARAAGADVVGFDDLAE
                     QVKGGTIDFDLCIATPDAMRVVGALGQILGPRGLMPNPKVGTVTMDVTTAVKNAKAGQ
                     VQYRTDKAGLIHATVGRASFTVEALQQNINALVDALVKAKPAAAKGVYLRRIALSSTM
                     GAGVRVEPTSLGAQQ"
     gene            complement(2460..2891)
                     /gene="rplK"
                     /locus_tag="PKX01_16750"
     CDS             complement(2460..2891)
                     /gene="rplK"
                     /locus_tag="PKX01_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMZ77837.1"
                     /translation="MAKKIIGYVKLQVPAGKANPSPPIGPALGQRGLNIMQFCKEFNA
                     ATQGVEPGLPIPVIITAFADKSFTFVMKTPPATILIKKAAGIQKGSSKPHTDKVGKIT
                     KAQCEEIAKTKMKDLTAADMEAAIRTIAGSARSMGITVEGL"
     gene            complement(2987..3520)
                     /gene="nusG"
                     /locus_tag="PKX01_16755"
     CDS             complement(2987..3520)
                     /gene="nusG"
                     /locus_tag="PKX01_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMZ77838.1"
                     /translation="MSKRWYVVHAYSGFEKSVQRALTERVARSGMQDLFGQILVPVEE
                     VVEMKNGQKSITERKFFPGYVLVEMEMNDDTWHLIKSTPKVTGFVGGTANKPTPISEK
                     EVAKIMQQMQEGVEKPRPKVLFEVGEMVRVKEGPFTDFNGSVEDVNYEKSKIRVSVTI
                     FGRATPVELDFGQVEKT"
     gene            complement(3517..3870)
                     /gene="secE"
                     /locus_tag="PKX01_16760"
     CDS             complement(3517..3870)
                     /gene="secE"
                     /locus_tag="PKX01_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMZ77839.1"
                     /translation="MADKIKFALALVLLVAGVVGFYLLAEQPMVLRVLSVLVGVGACA
                     AVAWKTEPGQRFAVFAGETVAEAKKVAWPSRKETFQMTGIVFAFVIIMAVFLWVTDKT
                     LEWALYDLILGWKKS"
     gene            complement(3896..3971)
                     /locus_tag="PKX01_16765"
     tRNA            complement(3896..3971)
                     /locus_tag="PKX01_16765"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3936..3938),aa:Trp,seq:cca)
BASE COUNT          762 a         1293 c         1227 g          796 t
ORIGIN      
        1 gtcatgagcg gaatctcgcc catgtacact tcctgctcct tgacctcctt gatggtttcc
       61 ttggccgctt ccttgtccag gatgaccagg cggacccggg cgcgcagcgg cgacgcgaaa
      121 gtcaggccgc gctgctggca ttccttcaca tcgaaggccg gctcgcccag gtgatactgg
      181 acaaattcca ggcgggcatt gccgctgtgg ctcgcgatgg ggaaaatcga gttgaaggca
      241 gcctgaagcc cctgggtggc ccgcgccgcc ggcggggttt ccgcctgcag gaaggacgca
      301 taggattcca gctgagtggc caaaagatag ggcacattct ttactgccac gcgcttggcg
      361 aagcttttac ggatgcgctt tttctcggtg taggagtacg ccatagttgc tccgggtctg
      421 agaagtcgga cgtcagaaga caaaagacag caattcgcga acttgattca gtatttcagg
      481 cgctataacc tgaatcgaat gctctttttt gctttctggc ggcaggttta cggttacgga
      541 aaacagaaag cacaaaaggg ccggtgccaa aaaggcacca gccccctgcg cgaagccggt
      601 attacttgac ttcgaccttg gcacccgcgt cttcgagttg cttcttgagc gcgtcggcgt
      661 cggccttggc gatgccttcc ttgacgggct tcggagcgcc atccaccaga tccttggctt
      721 ccttcaggcc caggccggtc acggcacgga ccaccttgat gacttcaacc ttcttgtcac
      781 cggcggcgag cagcatcacg gtgaattcgg tctgctcttc cacggcagca gcggcagcgc
      841 caccggcgcc cggagcggcc acagccatgg cggcggcgga cacaccgaac ttctcttcga
      901 acgccttgac caggtcgttc agttccatga cggtcatcgc gccaacggct tccaggatgt
      961 cttctttggt aattgccatt tcaacttact ccaattcgaa tttcgttcaa tcagccggcg
     1021 gctgcccgat caggcagcgg cgccttcttc gcgtttcttc gccagggcgg ccagcagacc
     1081 ggcaaagccg gacaccggcg cctgcatgac gcccagcagc ttggccagca gctcttcgcg
     1141 gctcgggatg gacgccagcg cctggacacc ttccttgtcg agcagcttgc ctgcatagca
     1201 acccgccttc aggaccagct tgtcgttggt cttggagaag tcggcgagca ccttggcggc
     1261 ggccacgggg tcggcggaga tgctgtagat cagcgggccg gtcaactgct cggccagcgg
     1321 agcgaagggc gtgtccgcca ctgcgcgccg tgcgagagtg ttcttcagca cgcgcaggta
     1381 gacacccgac gcacgcgcct tggcgcgcaa cgcggtgagg tcgccgacct cgatacccgc
     1441 gtactgcgcc acgacgatgg tctgggcgtt cgccacttgt gccgacacct cagccactac
     1501 ggcttttttg tcgtcgagat tcagactcaa ggtctttcct cctatcaagt caacacacga
     1561 cggaaagacc gtcgcaccta cggcgacctg gatcaggagc tttgcgcccg cgaacgtcgc
     1621 gtacgcgata aatcctgttc tgggacaccg tctacgcagg ctggaaaatt aagcttcggc
     1681 ggaagaatcc gccgaggcgc ctgcggtctt tgacgatcca gggcaccccg agaggcgcgc
     1741 cccggcccaa agttcttact gctgcgcgcc cagcgaagtg ggctcgacgc gaacgcccgc
     1801 gcccatcgtg gacgacaggg cgatacggcg caggtacacg cccttggctg cagccggctt
     1861 ggccttgacc agcgcgtcga ccagcgcatt gatgttctgt tgcagcgctt ccacggtgaa
     1921 ggaggcacga cccacggtgg cgtggatcag accggccttg tcggtacggt actgaacctg
     1981 accggccttg gcgttcttca cggcggtggt gacatccatg gtcacggtgc cgaccttcgg
     2041 gttcggcatc aggccacggg ggccgagaat ctgaccgagg gcgccgacga cgcgcatggc
     2101 atcgggcgtg gcaatgcaca gatcgaaatc gatggtgccg cccttgacct gttcggccag
     2161 atcgtcgaaa ccgaccacat cggcgccagc ggcacgggcc gcttcagcct tttcgccctg
     2221 ggcgaagacg gcgacgcgga cggacttgcc ggtaccggcc ggcagcacca cggagccccg
     2281 gacgatctgg tcggacttct tggcatccac gcccagattg acggcgatgt cgatggactc
     2341 gtcgaacttg gcggtggcgc attccttgac cagacccagg gcctcggtca ggggataggg
     2401 cttgttacgg tcgaccttgg cgcgcagggc ctgaacacgc ttggacagtt ttgccatgat
     2461 cacaggccct ccacggtgat gcccatgctg cgggcagagc cggcgatggt gcgaatggcg
     2521 gcttccatgt cggccgcggt cagatccttc atcttggtct tggcgatttc ttcgcactgg
     2581 gccttggtga tcttgccaac cttgtcggtg tgaggcttgg aggaaccctt ctggatgccc
     2641 gcggccttct tgatcaggat ggtggccggc ggcgtcttca tcacgaaggt gaagctcttg
     2701 tcggcgaagg cggtgatgat caccggaatc ggcagaccgg gttcgacgcc ctgggtggca
     2761 gcgttaaact ccttacagaa ctgcatgatg ttcaggccgc gctgaccgag tgccgggccg
     2821 atcggggggc tggggttggc tttgcctgca ggaacctgca gcttcacata gccgatgatt
     2881 ttctttgcca tctggcaact ccttggttga gttcaagcgt acggcgacat gccgcactcc
     2941 tcgcgccctg aatgggcgaa ggggcgcacc cggcgccccg acggggtcag gtcttctcga
     3001 cctgaccgaa atccagctcg accggcgtcg cacgaccgaa aatggtcacc gacacgcgga
     3061 tcttgctctt ttcatagttc acgtcctcga cggaaccatt gaaatcggtg aagggacctt
     3121 ccttgacacg caccatctcg ccgacctcga agagcacctt gggacggggc ttctcgaccc
     3181 cctcctgcat ctgctgcatg atcttcgcga cctccttctc ggagatcggc gtcggcttgt
     3241 tggccgtccc accgacgaag ccggtcacct tcggcgtaga cttgatgagg tgccaggtat
     3301 cgtcgttcat ctccatttcc acgagcacat agcccgggaa gaacttgcgt tcggtgatgc
     3361 tcttctggcc gttcttcatt tcgaccactt cctcgaccgg aacgaggatc tggccgaaca
     3421 agtcctgcat cccgcttcgc gccacccgct cggtgagcgc gcgctgaacc gacttctcga
     3481 agccggaata ggcatggacg acataccaac gcttgctcat gatttcttcc agcccaggat
     3541 cagatcgtaa agcgcccact cgagggtctt gtccgtcacc cacaggaaca ccgccatgat
     3601 gatgacgaag gcaaacacga tgccggtcat ctggaaggtc tccttgcggg agggccaggc
     3661 caccttcttc gcctcggcaa cggtctcgcc ggcaaacacc gcgaagcgct ggccgggctc
     3721 tgtcttccac gccacagccg cacaggcgcc cacgccgacc agcacggaca gaacgcgcag
     3781 caccatgggc tgctcggcga gcagatagaa gcccacaacg ccggccacca gtagcaccag
     3841 tgccagcgca aacttgattt tgtcggccat caaagcaatg aacccgagag aaacgtggca
     3901 ggggcagagg gaatcgaacc cccaaccttc ggttttggag accgacgctc tgccaattga
     3961 gctatacccc tgcagcagag actgcagggc gccccaacgg ggcgccctgc atacagcgat
     4021 cgagaattac tcgatgatct tggccacgac gccggcgccg acggtacgac cgccttca
//
LOCUS       DAOQPA010000202         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_14662_length_4053_cov_18.741871, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000202 DAOQPA010000000
VERSION     DAOQPA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14662_length_4053_cov_18.741871"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..3794)
                     /gene="metH"
                     /locus_tag="PKX01_16770"
     CDS             complement(93..3794)
                     /gene="metH"
                     /locus_tag="PKX01_16770"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMZ77840.1"
                     /translation="MQADRSTELQSLLTHRILILDGAMGTMIQQHRLGEADYRGARFA
                     EHPRDLKGNNDLLVLTRPDVVGGIHRAYLEAGADIIETCTFNATRVSQAEYGLAGLAY
                     ELNVEGARLVRALCDEFTAANPAKPRFCAGVIGPTSRTLSISPDVNDPGFRNIDFDAL
                     ADDYHEAARGLLDGGADILLIETVFDTLNAKAAVFAVEKLFAERGQRVPVMISGTITD
                     ASGRTLSGQTAEAFWNSLRHARPISFGLNCALGAKELRPYVEELANTCDCFVSAHPNA
                     GLPNPLSPTGYDETPEQLAAEIVEWARSGLVNIVGGCCGTSPAHIRAIAEAVRLVTPR
                     SVPEVAPRLRLSGLEPFNVGPDSLFVNVGERTNVTGSRAFARMILEGRFDDALAVARQ
                     QVENGAQVIDINMDEAMLDSLAAMERFLKLIAAEPDISRVPVMLDSSKWSVIEAGLKC
                     IQGKGVVNSISMKEGEAEFLRQARLCRQYGAAVIVMAFDEKGQADTYARKTEICRRAY
                     DLLTGIGFPAEDIIFDPNIFAIATGIEEHDNYAVDFIEATHWIRQHLPHAQVSGGVSN
                     VSFSFRGNDAVREAIHTVFLYHAIRAGMSMGIVNAGMLGVYDDLAPELRDKVEDVVLN
                     RHPGAGEVLVEFAQTVKEGRSKDAGPDLSWREQPVEARLSHALVKGITDFVVADTEEV
                     RARLEAAGQPPLAVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFI
                     EAEKLRTGAASKGRIVIATVKGDVHDIGKNIVGVVLGCNGYDVVDLGVMVSADKILHA
                     AREHGAQAIGLSGLITPSLEEMSHVAAEMQRQGFDVPLLIGGATTSRAHTAIKIAPHY
                     DQPVVYVPDASRAVGVVTRLLSDTQRSSFTAELTADYAKIRAQHAQKKGVALVPLGEA
                     RANAFRWNADPAYVPPVPARPGLHAFDIDLAELVDYIDWGPFFQTWDLAGRYPDILQD
                     ELVGETARELKSDAEVMLARMVAEQDTAPWVRARAVFGLFPAHAAGDDIVVYTDEARQ
                     DVRMTLHGLRQQHERPAGKPHYALADFVAPRDSGVADYVGAFAVTAGLDIEGRLAAYE
                     RSHDDYHAIMLKALADRFAEAAAEWLHARVRREYWAYAPAEALSNDELIREAYRGIRP
                     APGYPACPDHTVKGDLFRLLDAPAHAGMALTESFAMTPAASVCGLYLAHPDARYFAIS
                     KIGRDQLEDWARRKGMAVADAERWLAPLL"
     regulatory      complement(3864..3935)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
BASE COUNT          552 a         1296 c         1549 g          656 t
ORIGIN      
        1 ggtgccttcg cggatcaggc cgtgctcgtc gacagtcaga cgcacgcggg ggctccggca
       61 gcaggagcgc tctggagtct ggcccttccc gttcacagca gcggcgccag ccagcgctct
      121 gcgtccgcca ccgccatgcc cttgcgccgg gcccagtctt ccagctggtc gcggccgatc
      181 ttggagatgg cgaagtagcg ggcgtccggg tgggccaggt agaggccgca gacggaagcg
      241 gccggggtca tggcgaagct ctcggtgagg gccatgccgg cgtgggcggg ggcgtcgagg
      301 aggcggaaca ggtcgccctt gacggtgtgg tccgggcagg ccgggtagcc gggggcgggg
      361 cggatgccgc ggtaggcttc gcggatcagt tcgtcgttgc tcagggcctc ggctggggcg
      421 taggcccagt attcccggcg cacgcgggcg tgcagccact cggcggcggc ttcggcgaag
      481 cggtcggcga gggccttgag catgatcgcg tggtagtcgt cgtggctgcg ttcgtaggcc
      541 gccaggcggc cttcgatgtc caggccggcg gtgacggcga aggcgcccac gtagtcggcc
      601 acgccgctgt cccgcggcgc gacgaaatcc gccagggcgt agtggggctt gccggcgggg
      661 cgttcgtgct gctgacgcag gccgtgcaag gtcatccgca cgtcctggcg cgcctcgtcg
      721 gtgtagacga cgatgtcgtc ccccgccgcg tgggccggga agaggccgaa cacagcgcgg
      781 gcgcgcaccc acggcgcggt gtcctgctcg gccaccatgc gggcgagcat cacctcggcg
      841 tcggacttga gctcgcgggc ggtttcgccc accagttcgt cctggaggat gtccgggtag
      901 cggccggcca ggtcccaggt ctggaagaag gggccccagt cgatgtagtc caccagctcg
      961 gcgaggtcga tgtcgaaggc gtggagaccc ggccgggcgg gcaccggcgg cacgtaggcc
     1021 gggtcggcgt tccagcggaa ggcgttggcg cgggcctcgc ccaggggcac cagcgcgacg
     1081 cccttcttct gggcgtgctg ggcgcggatc ttggcgtagt cggcggtcag ctcggcggtg
     1141 aagctgctgc gctgggtgtc ggagagcagg cgcgtgacca cgcccacggc gcgggaggcg
     1201 tcgggcacgt agaccaccgg ctggtcgtag tggggcgcga tcttgatggc ggtgtgggcg
     1261 cggctggtgg tggcgccgcc gatcagcagg ggtacgtcga agccctggcg ctgcatctcg
     1321 gcggccacgt ggctcatctc ttccagcgac ggggtgatca ggccggacag gccgatggcc
     1381 tgggcgccgt gttcgcgggc ggcgtggagg atcttgtcgg cggacaccat gacgcccagg
     1441 tccaccacgt cgtagccgtt gcagccgagg accacgccga cgatgttctt gccgatgtcg
     1501 tgcacgtcgc ccttgaccgt ggcgatgacg atccggccct tgctggcggc gccggtgcgc
     1561 agcttttcgg cctcgatgaa ggggatcagg tgggccaccg cctgcttcat gacgcgggcg
     1621 gatttgacca cctggggcag gaacatcttg ccggcgccga agaggtcgcc caccacgttc
     1681 atgccggcca tcagcgggcc ttcgatgacc gccaggggcg gctggccggc ggcttcgagc
     1741 cgggcgcgga cttcctcggt gtcggccacc acgaagtcgg tgatgccctt gacgagggcg
     1801 tgggacaggc gcgcctcgac gggctgctcg cgccaggaca gatccgggcc ggcgtccttg
     1861 ctgcggcctt ccttgacggt ctgggcgaac tccaccagca cctcgccggc gcccgggtgg
     1921 cggttgagga ccacgtcctc caccttgtcg cgcagttcgg gcgccaggtc gtcgtagacg
     1981 cccagcatgc cggcgttgac gatgcccatg ctcatgcccg cccggatggc gtggtacagg
     2041 aagacggtgt ggatggcctc gcgcaccgcg tcgttgccgc ggaagctgaa gctcacgttg
     2101 gagacgccgc cgctcacctg ggcgtggggc aggtgctggc ggatccagtg ggtggcctcg
     2161 atgaagtcca ccgcgtagtt gtcgtgctcc tcgatgccgg tggcgatggc gaagatgttg
     2221 gggtcgaaga tgatgtcctc ggccgggaag ccgatgccgg tgagcaggtc gtaggcgcgc
     2281 cggcagatct cggtcttgcg ggcgtaggtg tcggcctggc ccttctcgtc gaaggccatc
     2341 acgatcaccg cggcgccgta ctggcggcac aggcgcgcct gacgcaggaa ttccgcctcc
     2401 ccttccttca tggagatgga attgaccacg cccttgccct ggatgcactt gaggccggcc
     2461 tcgatcacgc tccacttgga ggaatcgagc atcacgggca cccgggagat gtccggctcg
     2521 gcggcgatga gcttgaggaa gcgctccatc gcggccaggg agtcgagcat cgcctcgtcc
     2581 atgttgatgt cgatcacctg ggcgccgttt tccacctgct ggcgggccac ggccagggcg
     2641 tcgtcgaagc ggccttccag gatcatccgc gcaaaggcgc gggagccggt gacgttggtg
     2701 cgctcgccca cgttcacgaa gaggctgtcg gggcccacgt tgaagggctc caggcccgac
     2761 aggcgcaggc gcggggccac ctccggcacg ctgcgcgggg tgaccaggcg caccgcctcg
     2821 gcgatggcgc ggatgtgggc cggcgaggtg ccgcagcagc cgccgacgat gttcaccagc
     2881 ccgctgcgcg cccactccac gatctcggcg gcaagctgct ccggcgtctc gtcgtagccg
     2941 gtgggcgaca ggggattggg caggccggcg ttggggtggg ccgacacaaa gcagtcgcag
     3001 gtgttggcca gctcttccac gtaggggcgc agctccttcg caccgagggc gcagttgagg
     3061 ccgaagctga tgggccgggc gtggcgcagg gaattccaga aggcctcggc ggtctggccc
     3121 gagagggtgc gcccggaggc gtcggtgatg gtgccggaga tcatcaccgg cacgcgctgg
     3181 ccgcgctcgg cgaagagctt ctccacggcg aacaccgcgg ccttggcgtt gagggtgtcg
     3241 aacaccgtct cgatgagcag gatgtcggcg ccgccgtcca gcaggccgcg ggcggcctcg
     3301 tggtagtcgt ccgccagggc gtcgaagtcg atgttgcgga agcccggatc gttcacgtcc
     3361 ggcgagatgg acagggtgcg cgaggtgggc ccgatcacgc cggcgcagaa gcgcggcttg
     3421 gccgggttgg cggcggtgaa ttcgtcgcac agggcgcgca ccaggcgggc gccttccacg
     3481 ttgagctcgt aggccagccc ggccaggccg tattccgcct gggacacgcg ggtggcgttg
     3541 aaggtgcagg tctcgatgat gtccgcaccg gcctccaggt aggcgcgatg gatgccgccc
     3601 accacgtccg gacgggtcag caccagcagg tcgttgttgc ccttgagatc cctgggatgt
     3661 tccgcgaagc gcgcgccccg gtaatcggcc tcgcccagcc ggtgctgctg gatcatggtg
     3721 cccatcgccc cgtcgaggat caggatgcgg tgggtcagca gggactggag ttcggtgctg
     3781 cggtcggctt gcatggcggt tccttcgggt tcagccggat gcacgcgcgc tcaaacgcaa
     3841 acggcgccac aaacccggct gtcggtttgt gagcgccgtt gaatcggttg ccgagcctgg
     3901 cccgcgggtg acgggtcgca acgctcctcg gcaaggggcg gattttacgg ggtttttggc
     3961 ggggctgcca agggtgtgcg gtgcccctca ggcggccccg aggcgctcgg ccgcctcctc
     4021 ccgccaccac gccagcgcgg cgtaatagcc ttc
//
LOCUS       DAOQPA010000203         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_15970_length_3861_cov_18.779559, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000203 DAOQPA010000000
VERSION     DAOQPA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15970_length_3861_cov_18.779559"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..795)
                     /gene="purN"
                     /locus_tag="PKX01_16775"
     CDS             complement(151..795)
                     /gene="purN"
                     /locus_tag="PKX01_16775"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMZ77841.1"
                     /translation="MKNIVILISGRGSNMEAIVRARIDGARIAAVIANRPDAAGLQFA
                     AHAGIATRVVDHKAFASREAFDAALADVIDGFAPDLVVLAGFMRVLTDGFVQRYTGRL
                     LNIHPSLLPSFPGLHTHQRALEAGVRVHGTTVHFVTPALDCGPIVIQAAVPVRPGDDE
                     DRLAARVLVQEHRIYPQAVRWFVEGRLSLTPAGTVQVADVADGPDGWTVPPVEG"
     gene            complement(805..1452)
                     /locus_tag="PKX01_16780"
     CDS             complement(805..1452)
                     /locus_tag="PKX01_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ77842.1"
                     /translation="MELLSTFIDIVLHLDVHLAALTAQYGTWIYAILFAIIFCETGLV
                     IFPFLPGDSLLFVAGALAATGGMDITTLCGVLLAAAVLGDNTNYWIGHTLGPKVFRWE
                     NSRFFNRAAFDKTHAYFEVHGGKTIIVARFLPLLRTFAPFVAGVARMTYARFVALDFI
                     GGCVWILSLTWLGYGFGNLPEVKKNLSLVIVGIIALSLMPVVVGWLRGRSARAAN"
     gene            1731..2423
                     /locus_tag="PKX01_16785"
     CDS             1731..2423
                     /locus_tag="PKX01_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HMZ77843.1"
                     /translation="MSWPTLPAVAEGFAAAGSTLENVRRDFVEWHLGRPRYALWALDV
                     DTACVRRILDAAQGHLADLLLDTYRRQAHVTLALRGFPAATPRRHDEFGAAGLDADLA
                     ALTRRAPTSFLLETGGLASFTSAPFISVSDPDGGIARLRTALHDSDSDPGGPYTPHIT
                     VGLYAGCWPTGAVAERLHAHPLPTQTLHIERVCLMAYAPPEIGGPLETLGSFDLARRC
                     WTWHTATPFITP"
     gene            2594..3787
                     /locus_tag="PKX01_16790"
     CDS             2594..3787
                     /locus_tag="PKX01_16790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMZ77844.1"
                     /translation="MHPSLSLPLLALAFAAPLAQADAPRNYIVQFHDTAVQSPPRALV
                     RDHRVNSDPSGWAYLDARVMERLQSIESRHGIVARHAFSHVLKGFAATLTDAQLARLK
                     SDPAVMSIEEDLPVQIVGQTIPWGISTTRATLSSTLAGNGSGAVNGVNVYVIDTGIAP
                     HGDLNKVNHLNFTADGKNYDCHGHGTHVAGTIGARDDTTSVVGMAPYVRLTGVKVLDC
                     TGSGSDSTVIKGLDWVTANAVKPAVANMSLGGGASSALDAAVTRAVNAGVVFAIAAGN
                     SSAKACNYSPARVGGSLNGAITTAATDSSNKEASFSNYGSCVDTWAPGVSILSTYLNS
                     GVATMSGTSMASPHVAGAAALYLARNPTASPAAVEAAIKQAAVRTGTVSKDGRAITRL
                     DVIGF"
BASE COUNT          666 a         1376 c         1255 g          564 t
ORIGIN      
        1 gcgcgcgtcc agcggcggga gcacggtggc atcgtccgag ggcagacgcc aggccggact
       61 caggatcgcc agcccatgca ccagcaggga cagggccacg gcgatcgcga gggcccggtt
      121 gcggatccga ccgacggcca tccggcagtc tcagccctcg acgggcggca cggtccagcc
      181 gtcggggccg tccgccacat cggcgacctg gaccgtaccc gccggcgtga ggctgaggcg
      241 gccttcgacg aaccagcgca ccgcctgcgg gtagatgcgg tgctcctgca ccagcacccg
      301 cgcggcgagg cggtcttcgt cgtcgccggg gcgtaccggc accgcggcct ggatgacgat
      361 ggggccgcag tcgagcgcgg gcgtcacgaa atgcaccgtg gtgccatgca cgcgcacgcc
      421 ggcttccagc gcccgctggt gggtgtggag accagggaag gacggcagca gcgaggggtg
      481 gatgttgagg aggcggccgg tatagcgctg gacgaagcca tcggtgagca cgcgcatgaa
      541 gcccgccagc accaccagat cgggggcgaa gccgtcgatg acgtcggcca gggccgcgtc
      601 gaaggcttcg cggctggcaa aggccttgtg atccaccacg cgggtggcga tgccggcgtg
      661 tgcggcaaac tgcaggcccg ccgcgtcggg ccggttggcg atgaccgcgg cgatccgcgc
      721 gccgtcgata cgcgcccgca cgatggcctc catgttggag ccgcgcccgg agatgaggat
      781 gacgatattc ttcatgccgc cgcctcagtt cgccgcccgc gcactgcgac cgcgcagcca
      841 gccgaccacc accggcatca gggacagggc gatgatgccc acgatcacca gggacaggtt
      901 cttcttcacc tcgggcaggt tgccgaagcc gtagccgagc caggtgaggg acaggatcca
      961 cacgcagccg ccgatgaagt ccagggccac gaagcgggcg taagtcatgc gcgccacgcc
     1021 ggccacaaag ggcgcgaagg tgcgcagcag gggcaggaag cgggcgacga tgatggtctt
     1081 gccgccgtgg acttcgaagt aggcgtgggt cttgtcgaag gccgcgcgat tgaagaagcg
     1141 ggagttctcc cagcggaaca ccttgggacc gagggtgtgg ccgatccagt agttggtgtt
     1201 gtcccccagc accgccgccg ccagcagcac gccacacagg gtggtgatgt ccatgccgcc
     1261 ggtcgcggcc agggcgcccg ccacaaagag cagggaatcg cccggcagga agggaaagat
     1321 caccagcccc gtctcacaga agatgatggc gaacaggatg gcgtagatcc aggtgccgta
     1381 ctgggcggtc agggccgcca gatgcacgtc caggtgcagg acgatgtcga tgaaggtgct
     1441 gaggagttcc atgggcggtc gtacgaaatg cggtgcaaca tgataccgga ggcggtcggc
     1501 cggcgtgcgg acagacgccc gcccaccggc acaatggcgg ggaagaaact gtaagcggat
     1561 ttgccgcgca tacccgctcc gcgcccgcgc acccgatgcg gacgcttccc gcagggctgc
     1621 gccgatccgc ccgcgacccg acgccgggcc tgcggacgga aggccgcgcg atacgctcgc
     1681 cccgtgcaac gatgcattat gatcatcaga ttttcagcct tcagtcctgc atgtcctggc
     1741 ccaccctccc cgccgtcgct gaaggtttcg ccgccgcggg ttcgaccctc gaaaacgtcc
     1801 gccgggattt cgtggagtgg catctgggcc gcccccgtta tgccctgtgg gcactggacg
     1861 tcgatacggc atgcgtgcgc cggatcctgg acgccgccca gggccacctt gccgacctgc
     1921 tgctcgacac ctatcggcgc caggcccacg tcaccctggc cctgcgcggc tttccggccg
     1981 ccacgccgcg ccggcatgac gagtttggcg cggccggcct ggacgcggat ctcgccgccc
     2041 tcacgcggcg cgcgccgacg tccttcctcc tcgaaaccgg cggcctcgcc agcttcacgt
     2101 ccgccccgtt catcagcgtc tccgatcccg acggcgggat cgcccgcctg cgaacggccc
     2161 ttcacgacag cgatagcgat cctggcggcc cctatacacc ccacatcacg gtaggcctct
     2221 acgccggctg ctggccgacc ggggccgtcg ccgagcgcct ccatgcgcac ccactgccca
     2281 cgcagaccct gcacatcgag cgggtctgcc tgatggccta tgcgccgccg gagattggcg
     2341 gaccgctcga aacgctcggc agctttgatc tggcccggcg ctgctggacg tggcataccg
     2401 ccacgccctt catcaccccc tgaacggcgc atcggcggcc ggcaacgtgg gcccgagcgc
     2461 ggcgctgttg tcgcgaaccg cgtccgtgta aatcgtcaca aagtgtgtca aatgatcatc
     2521 aagccgcccg acccgcggcc gtagcaacgg aaggacccta gaatacgttt gtcattattc
     2581 cggaacccct cccatgcacc cttccctctc cctcccgctg cttgccctgg ccttcgcagc
     2641 acccctggcc caggccgatg cccccaggaa ctacatcgtc cagttccatg acacggccgt
     2701 acagtcgccc ccccgagccc tcgtccgtga tcatcgggtg aacagtgacc cctccggctg
     2761 ggcttatctg gacgcccgcg tgatggaacg cctgcagagc atcgaaagcc gccatggcat
     2821 cgtggcccgc cacgccttca gccacgtact caaaggcttc gccgccacgc tcacggatgc
     2881 ccagctcgca cgcctgaaga gcgatcctgc cgtgatgagc atcgaagagg atcttccggt
     2941 gcagatcgtc ggacagacca tcccgtgggg catcagcacc acccgtgcca ccctgtcatc
     3001 caccctggcc ggaaacggct cgggcgcggt caatggggtc aacgtctacg tcatcgacac
     3061 gggcattgca cctcacgggg atctgaacaa ggtcaatcac ctcaatttca cggccgacgg
     3121 caaaaactac gactgccatg gccacggcac ccacgtggcg ggcaccatcg gcgcccggga
     3181 cgacacgact tcggtggtcg gcatggcgcc ctatgtccgc ctgacggggg tcaaggtgct
     3241 cgactgtacg ggttcaggct cggattcgac cgtgatcaag ggactcgact gggtcaccgc
     3301 caacgcggtc aagcccgcag tggccaacat gagcctgggc ggcggcgcca gttcggccct
     3361 cgatgcggcc gtgacccgcg cggtcaatgc gggcgtcgtc tttgcaattg ccgccggcaa
     3421 cagcagcgcc aaggcctgca actattcgcc ggcacgcgtc ggcggctcgc tgaacggtgc
     3481 gatcaccacg gctgccaccg acagcagcaa caaagaggcc tcgttcagca actacggcag
     3541 ttgtgtggac acctgggcgc caggtgtttc gatcctgtcc acctacctca attccggcgt
     3601 ggccaccatg tcgggcacgt ccatggccag cccccatgtg gcaggcgccg cagcgctcta
     3661 cctggcgcgc aacccgacgg ccagcccggc cgccgtggaa gcggccatca agcaggcagc
     3721 cgtgcgcacc ggcaccgtca gcaaggacgg ccgcgccatc acccggctcg atgtgatcgg
     3781 cttctgacgc caccgcgacc accccgctcc cgtcatcgtt gatcgatggc gggacgccgc
     3841 acgcctcccc ctcacgcccc g
//
LOCUS       DAOQPA010000204         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_16041_length_3851_cov_19.539252, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000204 DAOQPA010000000
VERSION     DAOQPA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16041_length_3851_cov_19.539252"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKX01_16795"
     CDS             <1..411
                     /locus_tag="PKX01_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ77845.1"
                     /translation="PGTTAPAEPLTHQDAPTGSAASAPSSAPTPARDAVAPSLVAPRF
                     DADYLANPAPPYPGMSRRLGETGRVLLRVRVGSDGQPLDVGIAQSSGFARLDEAARAA
                     VARWRFVPARQGDQPVVASVQVPIVFTLNNKELP"
     gene            408..1142
                     /locus_tag="PKX01_16800"
     CDS             408..1142
                     /locus_tag="PKX01_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ77846.1"
                     /translation="MNESASAGLQLGQIWAQGDAIAHAVALLLLLMSLASWSVILIKG
                     WTLLRLRRLSTRSAQFWHCTRLEDGLTVLGPDGPDNPFRHLTERSTEAVAHHARHRDE
                     LHGVLDLNDWLSSSMRQTMDESTRRMQTGLSILASVGSTAPFVGLFGTVWGITHALTG
                     IGLSGQASLDQVAGPVGESLVMTAGGLAVAIPAVLAYNALTRGNREIVARLRHFAHDL
                     HAYLITGGKLRKRPVPPLDAPRQEAA"
     gene            1139..1561
                     /locus_tag="PKX01_16805"
     CDS             1139..1561
                     /locus_tag="PKX01_16805"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ77847.1"
                     /translation="MSPHGRFDDDADEGLLAEINMTPLVDVMLVLLIVFIITVPVIHH
                     AARLDLPTASSQPEDTRPPRITLSMQADGHVAWNGESLTRDGLESRLTDAARAQPQPE
                     LHLQVDRGTPYDHVAHLLADASRLGLARVAFVTEPGRR"
     gene            complement(2040..2324)
                     /locus_tag="PKX01_16810"
     CDS             complement(2040..2324)
                     /locus_tag="PKX01_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77848.1"
                     /translation="MGSSCNESYAEFLDSLKAAGVVIRNEDEVRERLAESQRWRNAFM
                     TLAANGRTIGIEFSVAGKSKNVGAVKRVLAQHAFPVEKEEALVAQLVADH"
     gene            complement(2439..3584)
                     /locus_tag="PKX01_16815"
     CDS             complement(2439..3584)
                     /locus_tag="PKX01_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HMZ77849.1"
                     /translation="MRISSNFDSGAIEVVSVDRADDIRLRLRADQGIEGDGAFRQWFH
                     FRVHGGAGAALRMVFENASEAAYPDGWPGYRCVASYDRRNWFRVSGTHYVDGQLVVEH
                     TPERDSVYYAYFEPYSHERHLDLLGWAEMSPYASVSNLGATVDGRDLDVVTVGRPGPG
                     KQPVWITARQHPGESMAEWFVEGVLERLLDGADPVARKIREHAVLHIVPNMNPDGAFR
                     GNLRTNAAGVNLNRAWREPDPLRSPEVHLVRQAMEASGVSLFLDIHGDEALPYVFFST
                     AEEVPGFSDAQRARQAEFVAAFEAASPDFQREHGYLPGRFAEELLTLASKWVAYRFGC
                     VSLTLEMPFKDNALLPDGLTGWNGARSKRLGAAILGPILRHVSATGA"
     gene            3727..3803
                     /locus_tag="PKX01_16820"
     tRNA            3727..3803
                     /locus_tag="PKX01_16820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3761..3763,aa:Pro,seq:ggg)
BASE COUNT          638 a         1489 c         1149 g          575 t
ORIGIN      
        1 cccggcacga ccgcgcctgc cgagcccctc acccaccagg acgccccgac cggcagcgcc
       61 gcatccgcgc cgtccagcgc cccgacgccc gcccgcgacg ccgttgcccc gtccctcgtc
      121 gccccccgct tcgacgccga ctacctggcc aatccggccc cgccctaccc cggcatgtcc
      181 cgccgtctgg gcgaaacggg gcgggtgctg ctgcgcgtgc gggtcggcag cgacggccag
      241 cccctcgacg tgggtatcgc ccagagcagc ggcttcgccc gcctcgacga ggccgcccgc
      301 gcggcggtcg cccgctggcg cttcgtcccg gcccgccagg gcgaccaacc agtcgtcgcc
      361 agcgtgcagg ttcccatcgt cttcaccctc aacaacaagg aactcccatg aacgaatccg
      421 cctcggccgg cctccagctc ggccagatct gggcccaggg cgacgccatc gcccacgcgg
      481 tcgccctgct cctgctcctc atgtccctgg cgtcctggtc cgtgatcctg atcaaaggct
      541 ggaccctcct gcgcctgcgc cgcctgagca cccgctccgc ccagttctgg cactgcaccc
      601 gcctggaaga cggcctcacc gtcctcggcc cggacggccc cgacaacccc ttccggcatc
      661 tcaccgagcg cagcaccgag gccgtcgccc accacgcccg ccaccgggac gagctgcacg
      721 gcgtcctcga tctcaacgac tggctctcca gctccatgcg tcagaccatg gacgaatcga
      781 cccggcgcat gcagaccggc ctgtccattc tcgcctcggt cggcagcacc gcccccttcg
      841 tcgggctgtt cggcaccgtg tggggcatca cccacgctct caccggcatc ggtctgtccg
      901 gtcaggccag cctcgaccag gtcgccggac cggtgggcga atccctggtg atgaccgccg
      961 gcggtctcgc cgtcgccatc cccgcggtgc tcgcctacaa cgccctcaca cggggcaacc
     1021 gggagatcgt cgcccgcctg cgccacttcg cccacgacct ccacgcctac ctcatcaccg
     1081 gcggcaagct gcgcaaacgc ccggtcccgc ccctcgacgc cccccgccag gaggccgcat
     1141 gagcccccac ggacgcttcg acgacgacgc ggatgaaggc ctcctcgccg agatcaacat
     1201 gacgccgctg gtggacgtga tgctggtcct gctgatcgtc ttcatcatca ccgttccggt
     1261 catccaccac gccgcgcgcc tcgacctgcc cacggcgagc agccagcccg aagacacccg
     1321 tccgccccgc atcaccctca gcatgcaggc cgacggacat gtggcgtgga acggcgaatc
     1381 cctgacgcgc gatggcctgg aaagtcgtct gacagacgcc gcccgtgccc agccccaacc
     1441 ggagctccat ctccaggtcg accgcggtac gccctacgac cacgtcgccc acctgcttgc
     1501 cgacgccagc cggctgggcc tggcgcgggt ggccttcgtc acggagccgg gccgacgctg
     1561 agcacagcgc cccgccagcc cgagaccttg acctgttccg cgacgaagcg ctcgcttgcg
     1621 acacgtcacg ccaaagcgga ccccgtttgc ccggatggcc gcggtgcgca cgagaagccg
     1681 gagcgacacc cggtcaggcc cggtagccag tggggacaga catggcgggg ggagacggat
     1741 cgaccggaca ccaatcccgc ccagcccccg acgcggaaac gtccagccgc gcagagcaag
     1801 gcatccttcc cccgccaccg cgtgttcacc gcagcgtggc gggggcgcag cgtcgagtgc
     1861 accggcacag gggtgaatcg gtggagcagc ccgcggcgcc aatcaacggg acgcgcctgt
     1921 gcccccgccg taggcgtgcc gggcgcccaa aagaaatgcc gtccggctgg ggccggacgg
     1981 catcggcatc ttctttcgat ccgtgcgcgg gcggcggaga cacccgccgc accgggggat
     2041 cagtggtcgg ctacgagttg ggcgaccagc gcttcttcct tttcaaccgg gaaggcgtgc
     2101 tgggccagca cccgcttcac cgcgcccacg ttcttggact tgccggcgac cgagaactcg
     2161 atgccgatcg tccggccgtt ggcagccagg gtcatgaacg cgttgcgcca gcgttgggat
     2221 tcggccagac gttcacgcac ttcatcttcg ttgcggatca caacacctgc ggctttgagg
     2281 gagtccagaa attcagcgta ggactcgttg caagaactgc ccatcgacat ctcctatggt
     2341 ctggtgcgtt gcacccgtac tgccactaac gcacacggaa accgtcagga tgacaggccg
     2401 ccgcaaaatt tctctcaaaa tgtttccgga cggggaactt atgcgcctgt cgcactgacg
     2461 tgccggagga tcggccccag gattgccgcg ccaaggcgtt tgctgcgcgc cccgttccag
     2521 cccgtcagcc cgtcgggcag cagggcgttg tccttgaagg gcatttccag cgtaagcgac
     2581 acgcaaccga agcgatacgc cacccatttg gacgccaggg tcagcaattc ctctgcaaaa
     2641 cgccccggca gatagccgtg ttcacgctga aaatccggac tcgcagcctc gaaggcggcg
     2701 acgaattccg cctgacgcgc ccgctgcgca tccgaaaacc ccggcacctc ctcggccgtc
     2761 gagaagaaca cgtagggcag cgcctcgtcg ccatggatgt cgaggaacag ggacaccccg
     2821 ctcgcctcca tggcctgccg gaccagatgg acctccgggc tgcgcagcgg gtccggctcg
     2881 cgccacgccc ggttcaggtt caccccggcc gcgttggtgc gcagattgcc gcggaacgcg
     2941 ccatccgggt tcatgttcgg cacgatgtgc aggaccgcat gctcacggat cttgcgcgcc
     3001 accggatcgg ctccgtccag cagacgttcc agcaccccct ccacgaacca ctccgccatg
     3061 ctctcacccg gatgctggcg cgccgtgatc cacaccggct gcttgccggg ccccgggcgc
     3121 cccaccgtca ccacatccag atcgcgccca tccaccgtgg cgcccaggtt gctcaccgag
     3181 gcatagggcg acatctccgc ccagcccagc agatccagat ggcgctcatg ggaatagggc
     3241 tcgaagtagg cgtaatagac gctgtcccgc tcgggcgtgt gctccaccac cagctgcccg
     3301 tccacataat gggtgccgct cacccggaac cagttccggc ggtcgtagga cgccacgcag
     3361 cgatagcccg gccagccgtc cggataggcc gcctccgacg cgttctcgaa gaccatccgc
     3421 agcgccgcgc ccgcgccccc gtgcacccgg aagtggaacc actgccggaa ggcgccgtcg
     3481 ccctcgatcc cctggtccgc ccgcagacgc agacggatgt cgtccgcccg atccaccgac
     3541 accacctcga tggcgcctga atcaaaattg ctgctgatac gaatcgtcat ggctgctccc
     3601 ggcgtccaaa gccggcacta tagcggcccg gtcccgatcc cggcagcagc aggcccgctc
     3661 ggccctgtgg ccaacgtgac aaggactttt caaacctgcg accctcgtct ataatgcgca
     3721 gcttgtcggg gcgtagcgca gcctggtagc gcacttgcat ggggtgcaag gggtcgcgag
     3781 ttcgaatccc gccgccccga ccattgattc aagcaaaaag ccaacctttc ggggttggct
     3841 ttttgctttg t
//
LOCUS       DAOQPA010000205         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_16982_length_3725_cov_18.565940, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000205 DAOQPA010000000
VERSION     DAOQPA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16982_length_3725_cov_18.565940"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PKX01_16825"
     CDS             <1..833
                     /locus_tag="PKX01_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HMZ77850.1"
                     /translation="APNQQAIPAVEYLMSKDGGEAKRFVLLGTDYVYPRTTNKILRAF
                     LHSKGVKDEDIMEDYTPFGHSDYQTIIAKIKKFASEGKKTAVVSTINGDSNVPFYKEL
                     GNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTKFVKMYKDW
                     AKKQKLPNADKVVTNDPMEATYIGIHMWAQAVEKAKSTDVDKVIAAMAGQTFKAPSGF
                     VAKMDEKNHHLHKPVFIGEVKADGQFNVVWKTPGPVRAAPWSPYIPGNDKKKDEPEGK
                     "
     gene            953..1396
                     /gene="cynS"
                     /locus_tag="PKX01_16830"
     CDS             953..1396
                     /gene="cynS"
                     /locus_tag="PKX01_16830"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HMZ77851.1"
                     /translation="MKREDVTDLILVRKLKKQLSWARLAEIVGQSKEWTTAALLGQMT
                     LSAAQAQAVGAALDLPDEAVEQLQIVPYKGSLPTAVPTDPLIYRFYELVNVYGTTFKA
                     LIHEEFGDGIMSAIDFHMDLTREPDPKGDRVKIVMSGKFLPYKTY"
     gene            1528..3108
                     /gene="urtB"
                     /locus_tag="PKX01_16835"
     CDS             1528..3108
                     /gene="urtB"
                     /locus_tag="PKX01_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HMZ77852.1"
                     /translation="MIKRLLFLLLCACSLAAHAALDAAQVRKLADDDSTEKVAAIRAL
                     VQSADPDALRVLQAMADDSLYVAGDKVVIVADDKAFDAASGAAIPMPDSPDSVTVNNR
                     IRGELAGALAALRLFDGDPAVRKDSAQKLQTGATPELAPLLARALDKESDAGIKALLA
                     TAHAQATLGSPDPAVRLAAVRALADSTSPTIRSQLQALTTVEGEADEKVRYEAVTAVK
                     AIESRLAFAENLGRVFSGLSLGSILLLIALGLAITYGVMGVINMAHGELLMVGAYSAY
                     AMQSVFRAYFPDAIDWYLPAAVPVAFLAAALVGVVMERTVIRWLYGRTLETLLATWGI
                     SLVLIQAARVLFGAQNVEVSNPSWMSGGIELMPNLMLPWNRIIIIGFALFVLTLVWAL
                     MNRTRLGMFVRAVTQNRAMAGCVGVPTSRIDTLAFALGAGIAGLGGVALSQISNVGPD
                     MGQGYIVDSFMVVVLGGVGQLAGAVWAALGLGVVSKLLEGWAGAVIAKIVVLVFIIVF
                     IQKRPQGLFALKGRFVDN"
     gene            3119..>3725
                     /locus_tag="PKX01_16840"
     CDS             3119..>3725
                     /locus_tag="PKX01_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HMZ77853.1"
                     /translation="MHKPLTLRILAALDAKGWLALGLFLAVTLVLVPVLHLTVSPDSP
                     FHVSAYAITLIGKIMCYALVAVAMDLIWGFGGILSLGHGLFFALGGYAFGMYLMRQIG
                     RDGSYQSDLPDFMVFLDWKELPWYWSGSDSFLWCALLVVGVPALVAFVFGYFAFRSRI
                     KGVYFSIITQALTYAAMLLFFRNETGFGGNNGFTDFKRVLGY"
BASE COUNT          621 a         1277 c         1211 g          616 t
ORIGIN      
        1 ccgcgcccaa ccagcaggcg attccggcgg tggaatacct gatgagcaag gacggtggcg
       61 aggccaagcg ctttgtgctg ctgggcaccg actacgtgta tccgcgcacc accaacaaga
      121 tcctgcgcgc cttcctgcac agcaagggtg tgaaggacga ggacatcatg gaggactaca
      181 cgcccttcgg tcacagcgac tatcagacga tcatcgccaa gatcaagaag ttcgcgtccg
      241 agggcaagaa gaccgcggtg gtgtccacga tcaacggcga ctccaacgtg cccttctaca
      301 aggaactggg caacgccggc ctgaaggcga cggacgtgcc ggtggtggcc ttctcggtgg
      361 gtgaagagga gctgcgcggc gtggatacca agccgctggt gggccacctg gcggcgtgga
      421 actacttcat gtccctgaag aacccggaaa acaccaagtt cgtgaagatg tacaaggact
      481 gggcgaagaa gcagaagctg cccaacgccg acaaggtggt gaccaacgac ccgatggaag
      541 ccacctacat cggcatccac atgtgggccc aggccgtcga gaaggccaag agcaccgatg
      601 tggacaaggt catcgccgcc atggcgggcc agaccttcaa ggcgccgtcg ggcttcgtcg
      661 ccaagatgga cgagaagaac caccacctgc acaagccggt gttcatcggc gaagtgaagg
      721 cggacggcca gttcaacgtg gtgtggaaga cccccggccc ggtccgcgcg gccccgtgga
      781 gcccctacat ccctggcaac gacaagaaga aggacgagcc ggaaggcaag taatcccgtc
      841 cgatgtcgtg agcgtcgcac gaaacggggg ctgcggcccc cgttttgtcg tgcctgacgc
      901 gcccgcagcc ccgcaccgcc cgccgtcccc cgcccaattc cggagaatcc ccatgaagcg
      961 tgaagatgtc accgacctga tcctcgtccg caagctgaag aagcagctca gctgggcccg
     1021 cctcgccgag atcgtcggcc agagcaagga atggacgacc gccgccctgc tcggccagat
     1081 gaccctcagc gccgcccagg cccaggccgt gggcgccgcc ctcgacctgc ccgacgaggc
     1141 cgtggagcag ctgcagatcg tgccctacaa gggcagcctg cccaccgccg tgcccaccga
     1201 tccgctgatc taccgcttct acgagctggt gaatgtgtat ggcaccacct tcaaggccct
     1261 gatccacgag gaattcggcg acgggatcat gagcgccatc gatttccaca tggacctgac
     1321 ccgcgaaccg gaccccaagg gcgaccgggt gaagatcgtc atgagcggca agttcctgcc
     1381 ttacaagacc tactgatgcg ccgcggccgg cgccccccgg cggggccgcc gccgcgaacg
     1441 gcccctacgc aattcatcgt attcaagcct gcgggcctgc tccgtaagct ctctcctgca
     1501 ctgcaccaat ctgcggacgg accgttcatg atcaaaagac tcctcttcct gctgctctgc
     1561 gcctgcagcc tcgcggccca cgccgcgctg gacgcggccc aggtgcggaa actcgccgat
     1621 gacgattcca ccgagaaggt ggcggcgatc cgtgcgctcg tccagagcgc cgacccggac
     1681 gccctgcggg tgctccaggc gatggccgac gacagcctct atgtggccgg cgacaaggtg
     1741 gtgatcgtcg ccgacgacaa ggccttcgac gccgccagcg gcgccgccat cccgatgccc
     1801 gacagcccgg actcggtgac ggtgaacaac cgcatccgcg gcgagctggc cggcgccctg
     1861 gcggccctgc gtctcttcga cggtgacccg gcggtgcgca aggattccgc ccagaagctc
     1921 cagaccggcg cgaccccgga gttggcgccc ctcctcgccc gcgcgctgga caaggaaagc
     1981 gacgccggca tcaaggccct gctcgccacc gcccacgccc aggcgactct gggcagcccc
     2041 gatccggcgg tgcgtctggc ggccgtgcgg gccctggcgg acagcacctc gccgaccatc
     2101 cgcagccagc tgcaggccct caccaccgtc gagggggagg cggacgagaa ggtgcgctat
     2161 gaggcggtga cggcggtcaa ggccatcgaa tcgcgcctgg cctttgccga gaacctcggg
     2221 cgggtcttct ccggcctgtc cctgggcagc atcctgctgc tcatcgccct gggcctggcc
     2281 atcacctacg gggtgatggg cgtcatcaac atggcccacg gggagctgct gatggtgggc
     2341 gcctactccg cctatgccat gcagagcgtg ttccgcgcct acttcccgga cgccatcgac
     2401 tggtatctgc ccgccgcggt gccggtggcc ttcctggccg cggcgctggt gggcgtggtg
     2461 atggaacgca cggtgatccg ctggctgtac ggccgcacgc tggagaccct gctcgccacc
     2521 tggggtatct cgctggtgct gatccaggca gcgcgggtgc tcttcggcgc ccagaacgtg
     2581 gaggtctcca accccagctg gatgtccggt ggcatcgagc tgatgcccaa cctgatgctg
     2641 ccctggaacc gcatcatcat catcggcttt gccctcttcg tgctgaccct ggtgtgggcg
     2701 ctgatgaacc ggacccgcct gggcatgttc gtgcgcgccg tgacccagaa ccgggccatg
     2761 gcgggctgcg tgggcgtgcc cacgtcgcgc atcgacaccc tggccttcgc cctgggcgcc
     2821 ggcatcgccg ggctgggcgg cgtggccttg tcccagatca gcaacgtggg accggacatg
     2881 ggccagggct acatcgtgga ttccttcatg gtggtggtgc tgggcggtgt cgggcagctg
     2941 gccggcgcgg tgtgggcggc cctcggtctg ggggtcgtct ccaagctgct ggaaggctgg
     3001 gccggcgcgg tgatcgccaa gatcgtcgtc ctcgtcttca tcatcgtctt catccagaag
     3061 cgtccgcagg ggctgttcgc cctgaagggc cgcttcgtcg acaactgaga gcccggccat
     3121 gcacaaaccc ctcacgctcc gcatcctggc ggccctcgac gccaagggct ggctggccct
     3181 cgggctgttc ctggcggtga ccctggtgct ggtgccggtg ctgcacctga ccgtgtcgcc
     3241 ggacagcccc ttccacgtgt ccgcctacgc catcaccctg atcggcaaga tcatgtgcta
     3301 cgccctggtg gcggtggcga tggacctgat ctggggcttc ggcggcatcc tgtccctcgg
     3361 ccacgggctg ttcttcgccc tcggcggcta cgccttcggg atgtacctga tgcgccagat
     3421 cggccgcgac ggcagctacc agagcgatct gccggatttc atggtgttcc tcgactggaa
     3481 ggagctgccc tggtactgga gcggcagcga cagcttcctg tggtgcgccc tgctggtggt
     3541 gggcgtgccg gcgctggtgg ccttcgtgtt cggctacttc gccttccgct cgcggatcaa
     3601 gggcgtgtat ttctcgatca tcacccaggc cctcacctac gcggcgatgc tgctgttctt
     3661 ccgcaacgag accggcttcg gcggcaacaa cggcttcacc gacttcaagc gcgtgctggg
     3721 ctatt
//
LOCUS       DAOQPA010000206         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_17434_length_3673_cov_9.192924, whole genome shotgun sequence.
ACCESSION   DAOQPA010000206 DAOQPA010000000
VERSION     DAOQPA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17434_length_3673_cov_9.192924"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKX01_16845"
     CDS             complement(<1..395)
                     /locus_tag="PKX01_16845"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HMZ77854.1"
                     /translation="MFLSDEIASTAHLSLPEYQSRASASNQFKGTVEGFNQLRYGFFG
                     EVGGILAAIKKSKRDLGPAEQANVTEELGDALWYLTTVAVECKHSLNEVGTAALKELQ
                     RRLEVVHTRPTGNLTFAEFDGLTAFCHSKL"
     gene            753..1079
                     /locus_tag="PKX01_16850"
     CDS             753..1079
                     /locus_tag="PKX01_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ77855.1"
                     /translation="MQKNRKSVQEAEELGGALRRRRKELGLTLSSLADILQIDAGQLS
                     RFERAEFKRASRNLQKVAIFLQIPVAKETGESNAVVRQFAELLGRSERHRAAAIALVL
                     ALQELR"
     gene            complement(1389..1781)
                     /locus_tag="PKX01_16855"
     CDS             complement(1389..1781)
                     /locus_tag="PKX01_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ77856.1"
                     /translation="MFSHIFVGTNDFERALAFYRPLMDVLGIQARFCDPARPWAGWQS
                     APDPRPLFLLGRPWDGQPHTPGNGQMVAFLAPSRAAVADAHRLALAYGGRDEGAPGLR
                     PEYHAHYFGAYFRDPDGNKVCVVCHAPV"
     gene            complement(1938..2495)
                     /locus_tag="PKX01_16860"
     CDS             complement(1938..2495)
                     /locus_tag="PKX01_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ77857.1"
                     /translation="MPAAIVPIPSFVPKVMTVPTVTPLSELHADIDRRVATLRSEHAD
                     WPCAKGCDTCCRRLADVPRLTASEWGLLREGLAALPAPRRAGFRAAVARLADTPERPL
                     TCPFLDPVSGACSVYAYRPVACRTYGFYADRDGGLYCHDIEARVDDGRLAGVVWGNHA
                     AVLTRLARTGETRPLTEWYADWHET"
     gene            2863..>3673
                     /locus_tag="PKX01_16865"
     CDS             2863..>3673
                     /locus_tag="PKX01_16865"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HMZ77858.1"
                     /translation="MLVLCLAYAVTGWLALQIAVPPGYAAPLFPPAGIALAAVMLYGP
                     SCLPAIFLGSLLVQVPAAIQTGIDIQRWPGLLVPAFGAMLQARVGYGLARRLIGLPNT
                     LDSQDSILNFVALVAPLGCVVSASIAVPTLVLMHAIPPSESTFTWINWWAGDTLGVLV
                     MVPLTLVLFGLPAADWRPRRLTVAVPMSVALTLLAVTFMQVREWETQRLQTQFNRDAE
                     FVASLVQKRFDAQLDQLQALERLTAVHPGLSADEWRDFVTPMMARNPGTQNF"
BASE COUNT          656 a         1237 c         1155 g          625 t
ORIGIN      
        1 agcttgctgt ggcagaacgc agtcaaaccg tcaaactcgg caaacgtaag atttccggtg
       61 ggcctagtat gcactacctc aagccgacgc tggagttcct taagcgcggc agtccccact
      121 tcgttcaaac tgtgcttgca ctctactgcg acagttgtca gataccaaag cgcatcgccg
      181 agttcctcgg tcacattcgc ctgctcggcc gggccaaggt ctcgtttgga tttcttgata
      241 gcggccagta ttccgccgac ctccccaaag aaaccatacc ggagttggtt gaatccttcc
      301 acagttccct tgaactggtt cgaagcagat gcgcgcgact ggtactcggg gagcgacaga
      361 tgtgccgtac ttgcaatctc atcggataga aacatgacgg agaccttcct ggccgttgtt
      421 gtcctatgac tgtggcacag cctacctctc ggcgtaaagc tcggctttca gccgctttgc
      481 acacgtaatg ataatgcttc ggtcacccat cgatgcacca agcgccaggg ccttgtctcg
      541 aattcccaca gtgacatcgt agtgaggata gaccgattgc tcttgaaacc aggaccggcg
      601 tagtccaagc cgccgagcga actcgtgtaa ctcgggcagc gaatcggcaa ccagatggca
      661 ccactgcgtg cctttccacg ggatgcactc gttgtctacg tagatagtca tacttgccaa
      721 cttacaaaaa tatttgcaaa ataatatgca acatgcagaa aaatcgcaag tctgttcagg
      781 aagccgaaga gctcggcggc gctttgcgtc ggcggcggaa ggagcttgga ctgacgctta
      841 gcagtctggc agacatcttg caaattgatg ctggccaact ttcaagattc gagcgtgcag
      901 agttcaagcg tgcatccaga aatttgcaaa aagttgcaat atttttgcaa ataccggtgg
      961 ctaaagagac gggggagtcc aatgctgtcg ttcggcagtt tgctgagctt cttggtcgct
     1021 ccgaaaggca tagggcagct gcaatcgcat tggttctagc gcttcaagag cttcggtagc
     1081 tacataggct ttgagggcga cgatgcgtat tgggagaaaa tcgaacgcgc ccgcccgatt
     1141 ctcaatgccg acctttaccc caaactcgta gggctgccgg gctttgccct tgccgatgca
     1201 ctccccttca ggtcctgcgc aagtccgtgg aacctgccga actgcaggca gtgctgggcc
     1261 actggctgtc cggctcataa tctggtcgcg accgggtccg gccgccgcca cgcgcgagca
     1321 gcgacccgcc gccaacacga ccgctgcgcg cctgtgcgcg ccgcggcggc gcccagccag
     1381 acggcgcctt ataccggggc gtggcacacc acgcacacct tgttcccgtc cggatcgcgg
     1441 aaatacgccc cgaagtagtg tgcgtggtac tccgggcgca ggccaggcgc accctcgtcg
     1501 cggcccccgt acgccagggc gaggcggtgg gcgtccgcca cggcggcgcg gcttggcgcc
     1561 aggaaggcca ccatctgccc gttgccgggc gtgtggggct gaccgtccca ggggcggccc
     1621 aggaggaaca ggggacgtgg atccggcgcc gactgccagc cggcccacgg ccgcgccgga
     1681 tcgcagaaac gggcctggat gccgaggacg tccatcaagg ggcgataaaa ggcgagggcg
     1741 cgttcgaaat cattggtgcc gacgaagatg tgggaaaaca tggctgctcc aggaaacagg
     1801 ggaagatccg gacccgccgg acccgatccg ctcgacaccg cgacaccccg cgggggcgga
     1861 tgagcgcccc cccgcggacg ggccgacggc gccgggcatc gggcggcgcg gtgcgacggg
     1921 cggagcgccc gcgcggttca ggtttcatgc cagtccgcgt accactcggt caggggccgc
     1981 gtctcgccgg tcctggcgag gcgggtgagc accgccgcat ggttgcccca gaccaccccg
     2041 gccagacgcc cgtcgtccac ccgggcctcg atgtcatggc agtacagccc gccgtcccgg
     2101 tcggcataga agccgtaggt gcggcaggcc accgggcgat aggcataaac cgagcaggca
     2161 ccactcaccg ggtcgaggaa ggggcaggtc agggggcgct ccggcgtatc ggccaggcgc
     2221 gccactgccg cgcgaaagcc ggcccgccgg ggcgctggga gggcggccag gccttcccgc
     2281 agcagccccc attcggacgc ggtaagacgg ggcacatcgg cgagccgccg gcagcaggtg
     2341 tcgcagccct tggcacaggg ccagtctgca tgctcactgc gcagggtggc gacacgccga
     2401 tcgatgtcgg catggagttc ggacaggggg gtgacggtgg gcacggtcat gaccttcggc
     2461 acgaaggagg ggatcggtac gatagcagcc ggcatccgcg gcgggaatcc gccccgacgg
     2521 gaccgccgat gcgccgattc cggacggacg cgcgccgcga cacgcgccct cagccggatc
     2581 ggaccgcaac ccacgccggc gccggacccg gatcggccgc ctctccggcc cggcccatcg
     2641 agacgggtaa gacgtaccgg atcgcccctc accgcgccct gcacccttgt ccgccgggcg
     2701 ccccaggtcc ggcacgcatc gatccatcgc cctgcacgtc actccgacgc caatcggtcg
     2761 taaaataagg ttaaatgaca ggctgtcgtc ccctgcatca ctccacgtga accgtctgcc
     2821 cacctgatgc atttcccccc gcggcatcgc acgctgtttc gcatgctggt cctgtgcctc
     2881 gcctatgccg tcaccggctg gcttgcgctg cagatcgccg tcccgcccgg ttacgcggca
     2941 ccgctcttcc cccccgccgg catcgcgctg gccgcggtca tgctctacgg gccgtcctgc
     3001 ctgccggcga tctttctcgg atcgctgctg gtccaggtgc cggccgccat acagaccggc
     3061 atcgacatcc agcgctggcc ggggctgctg gtgccggcct tcggcgcgat gctgcaggcc
     3121 cgcgtcggct atggcctggc acggcgcctg atcggactgc ccaacaccct ggacagtcag
     3181 gacagcatcc tgaatttcgt cgccctggtg gccccgctgg gctgtgtcgt cagtgccagc
     3241 atcgcggtgc cgacactcgt gctgatgcac gccatcccgc cctccgagag caccttcacg
     3301 tggatcaact ggtgggccgg cgacaccctc ggcgtgctgg tgatggtgcc cctgaccctg
     3361 gtcctgttcg gcctgcccgc tgcggactgg cgcccccggc gcctcaccgt cgccgtcccc
     3421 atgagcgtcg ccctgacgct cctggcggtc accttcatgc aggtgcggga atgggaaacc
     3481 cagcgcctgc agacccagtt caaccgggat gccgaattcg tcgccagcct ggtgcagaag
     3541 cgcttcgacg cccagctcga ccagttgcag gcgctggagc gcctcaccgc ggtgcacccc
     3601 ggactcagcg ccgacgaatg gcgcgatttc gtcacgccga tgatggcccg caatccgggc
     3661 acccagaact tcg
//
LOCUS       DAOQPA010000207         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_18316_length_3572_cov_16.454080, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000207 DAOQPA010000000
VERSION     DAOQPA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18316_length_3572_cov_16.454080"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKX01_16870"
     CDS             complement(<1..721)
                     /locus_tag="PKX01_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ77859.1"
                     /translation="MLYALPGTAEAKVQYKSRYDNFIGGKWVAPLKGEYFDVITPITG
                     KPYTQVARSQAEDVELALDAAHAAADKWGKTSPAERSNILLKIADRLEANLEKLAYAE
                     TVDNGKPIRETLNADIPLAVDHFRYFAGCLRAQEGTISEINEDTVAYHIHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPISILVLAELIADLLPPGVLNIVNG
                     FGREAGMPLATSKRIAKIAFTG"
     gene            1032..1739
                     /gene="rquA"
                     /locus_tag="PKX01_16875"
     CDS             1032..1739
                     /gene="rquA"
                     /locus_tag="PKX01_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HMZ77860.1"
                     /translation="MSSQPSADQAPAAVIEPSLPDYLRETYTWAYLDPRTVPWLDRPL
                     VVSAILWGNARRLMDAAVAEFTPGQRVLQAAAVYGDFSVRLARQLGPEGALTVVDVAR
                     IQVANTRRKLEAWPTARVLRADLTEPQGTTFDAVCCFFLLHEVPPHARQRIVGNLLAA
                     VEPGGKIVFVDYHRPRWWHPMGPVMALVFRFLEPYARSLLAESIDTLVPAAADFDWHS
                     ETRFGGLYQKVVGVRRR"
     gene            complement(1750..2550)
                     /locus_tag="PKX01_16880"
     CDS             complement(1750..2550)
                     /locus_tag="PKX01_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HMZ77861.1"
                     /translation="MPRLPCRIVCLSTETVEVLYLLGEAARIAGISGFTTHPPQARKE
                     KPKVSGFSSAKIDRILAVEPDLVLAFSDLQGEIARDLVRAGVAVHVFNQRSVDGILAM
                     VETVGRLVGAEARALALVAGLETDIAAARAAGEARIARLGRRPRVYFEEWNDPLISGI
                     RWVSELIHLAGGEDCFAELAVHAGAKARILSGPEAVIPRAPEVIVGSWCGKHFQPAQV
                     LARPGWDALPAVRDGHVYEVKSAILLAPGVAAIREGLPALAACLDRLG"
     gene            complement(2574..>3572)
                     /locus_tag="PKX01_16885"
     CDS             complement(2574..>3572)
                     /locus_tag="PKX01_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77862.1"
                     /translation="ARAEGGPLPGEQLLGPGQLAPLPADARVVPGYAGTWRRADSQER
                     VRDRIVELEGRRAKPCTDVRVTDTEQAAPDLAETRPTRIHHALERWTVRACGATRSYE
                     VWYRFAPGASRVAVAENGGGRFQGELDPPYRRLLELAAAHPRDAGAGTDPRRWLNLPL
                     PEGWHYASQSGGLPGQDWRTDFLPVGPRLPEWTDLISVQGLPRRPATGQARLYLEGLQ
                     ASRQTRCGAAPEPIEALPADDASGRARLRTLLICPQVPGTDYAELALVQALEGPDILY
                     VVQRAWRLPAADADSLRRDSADVLKTGAYFLSLVRLCPPATRMRDCPAPFPADLRP"
BASE COUNT          491 a         1160 c         1345 g          576 t
ORIGIN      
        1 agccggtgaa ggcgatcttg gcgatgcgct tgctggtggc cagcggcatg ccggcttcgc
       61 ggccaaagcc gttgacgatg ttcagcacgc ccgggggcag caggtcggcg atcagctcgg
      121 ccagcaccag gatggagatc ggggtggatt cggcgggctt caggaccacg cagttgccgg
      181 cgcccagcgc gggggccagc ttccaggcgg ccatcaggat cgggaagttc caggggatga
      241 tctggccgac gacgcccagg ggctcatgga tgtggtaggc gacggtgtct tcgttgatct
      301 cggagatggt gccttcctgg gcgcgcaggc agccggcgaa gtagcggaag tggtcgacgg
      361 ccagggggat gtcggcgttc agggtttcgc ggatcggctt gccgttgtcg acagtttcgg
      421 cgtaggccag cttctcgagg ttggcttcga ggcggtcggc gatcttcagc aggatgttgg
      481 agcgctcggc gggggaggtc ttgccccact tgtcggcggc ggcgtgggcg gcgtcgaggg
      541 ccagttccac gtcttcggcc tgggagcggg cgacctgggt gtagggcttg ccggtgatcg
      601 gggtgatcac gtcgaagtat tcacccttca gcggggcgac ccacttgcca ccgatgaagt
      661 tgtcgtaacg ggacttgtat tggaccttgg cttcggcggt gccgggcagt gcgtacagca
      721 tggaaatctc ctcctcgagt ggattgataa cgcggtgttg ggcgttcaat agagatcagc
      781 aggccgcgtg ccatacgggg aggtggggcg gtagaaaatg aattgttcaa tgtattcatg
      841 aagttgatgc tattcatgtt gcattgcaac gtccttggag cctgtggagc acctgttcat
      901 tttcgcgaca ggtgctccat cctgcgtgtg caggcgggac agcgccttcc cgcgccgcca
      961 gccccatgga tccatggtgc cggtgcgcgc gcggtggcat catggcggtt cctgcatcga
     1021 ccagcgtctc catgtcctcc cagccttcgg cggatcaggc ccccgcggcc gtcatcgaac
     1081 cttccctgcc cgactacctg cgcgaaacct acacctgggc ctatctggac ccgcgcaccg
     1141 tgccctggct cgaccgtccc ctggtggtgt cggcgatcct gtggggcaac gcccgccgcc
     1201 tgatggatgc cgccgtggcg gaattcactc ccggccagcg ggtgctccag gcggccgccg
     1261 tgtatggcga cttctccgtg cgcctggcgc gccagttggg gccggagggg gcgctgaccg
     1321 tggtggatgt ggcgcgcatc caggtggcca acacccgtcg caagctcgaa gcctggccca
     1381 cagcgcgggt gctgcgggcg gatctcaccg agccccaggg caccaccttc gacgcggtgt
     1441 gctgcttctt cctgctccac gaggtgccgc cccacgcccg ccagcgcatc gtcggcaatc
     1501 tgctggcggc ggtggagccg ggcggcaaga ttgtcttcgt cgattaccac cggccgcgct
     1561 ggtggcatcc gatggggccg gtgatggcgc tggtcttccg ctttctcgaa ccttacgcgc
     1621 gttccctgct ggccgaatcc atcgacaccc tggtgccggc cgcggccgac ttcgactggc
     1681 acagcgagac ccgcttcggc gggctctacc agaaggtggt gggcgtacgc cggcgctgaa
     1741 gcgccggatt cagccgaggc ggtccagaca ggccgccagc gcgggcaggc cttcgcggat
     1801 cgccgccacg ccgggggcca gcaggatcgc cgacttcacc tcgtagacat gaccgtcgcg
     1861 gaccgccggc agggcgtccc agcccggtcg ggcgagtacc tgggcgggct ggaagtgttt
     1921 gccgcaccag gagccgacga tcacttccgg cgcccgcgga atcaccgcct ccgggccgga
     1981 gaggatgcgc gccttcgcgc cggcatgcac tgccagctcc gcgaagcagt cttcgccccc
     2041 ggcaaggtgg atcagctccg acacccagcg gatgccgctg atgaggggat cgttccattc
     2101 ctcgaaatac acccgcggac gtcgccccag gcgggcgatg cgggcctcgc cggcggcccg
     2161 ggcggcggcg atgtcggtct ccagccccgc caccagggcg agggcccggg cctcggcgcc
     2221 caccagccgg ccgaccgtct cgaccatggc caggatgccg tccacgctgc gctggttgaa
     2281 gacatgcacc gcaacgcccg cccgcaccag atcccgcgca atctcgccct gcaggtcgga
     2341 gaaggccagc accaggtccg gttcgacggc caggatgcgg tcgattttgg cgctggagaa
     2401 gccgctgacc ttgggctttt ccttgcgcgc ctggggcggg tgggtggtga agccggagat
     2461 gccggcgatg cgggccgcct caccgagcag atagagcacc tcgacggtct cggtggacag
     2521 gcagacgatg cggcagggca gtcggggcat ggcggacgtc gggtgggggc ggttcagggg
     2581 cgcagatcgg ccgggaaggg cgccggacag tcgcgcatgc gcgtggccgg cgggcacagg
     2641 cggacgaggg acaggaagta ggcgccggtc ttcagcacgt cggcgctgtc gcggcgcagg
     2701 ctgtccgcgt cggcggcggg caggcgccag gcgcgctgca ccacgtaaag gatgtccgga
     2761 ccctcgaggg cctgcaccag ggccagctcg gcgtagtcgg tgccgggcac ctgggggcag
     2821 atcagcaggg tgcgcagacg cgcgcgcccg ctcgcgtcgt cggcggggag cgcttcgatg
     2881 ggctcgggcg ctgcgccgca gcgggtctgc cgactggcct gcagaccttc aagatagagg
     2941 cgtgcctgac cggtggcggg gcggcggggc aggccctgca cgctgatcag gtcggtccat
     3001 tcgggcaggc gcgggccgac gggcaggaag tcggtgcgcc agtcttgacc ggggaggccg
     3061 ccgctctggc tggcgtagtg ccagccttcg ggcaggggca ggttgagcca gcgccggggg
     3121 tcggtgcccg cgcccgcgtc ccgtgggtgg gccgcggcga gttcgagcag ccggcggtag
     3181 ggcgggtcga gttcgccctg gaagcggccg ccaccgtttt ccgccaccgc cacgcgggag
     3241 gcgcctggcg cgaagcggta ccacacctcg tagctgcgcg tcgcgccgca ggcccgcacg
     3301 gtccatcgtt cgagggcatg gtggatccgc gtcgggcggg tttccgccag gtccggcgcg
     3361 gcctgttcgg tgtcggtgac gcgcacgtcg gtgcagggct tcgcgcgccg gccttccagt
     3421 tccacgatgc gatcgcgtac ccgctcctga ctgtcggccc gccgccaggt accggcgtag
     3481 ccgggcacca cgcgggcgtc ggcgggcagc ggggcgagct ggccggggcc gaggagctgt
     3541 tcgccgggca gcgggccgcc ctcggcgcgg gc
//
LOCUS       DAOQPA010000208         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_18987_length_3499_cov_16.835075, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000208 DAOQPA010000000
VERSION     DAOQPA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18987_length_3499_cov_16.835075"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..968)
                     /locus_tag="PKX01_16890"
     CDS             complement(21..968)
                     /locus_tag="PKX01_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HMZ77863.1"
                     /translation="MGDRIDVRTAAAGRWRAILLSLGVDERALSGKHGPCPLCGGRDR
                     FRFDDKDGRGTYFCSGCGAGDGVRLAMGITGLSFKDAAREIERLAGVVQPVTGKPDRT
                     DDDKLAALRRVWRESKPIQRGDEAYAYLAGRGLRLYDLPEAVRLHPGLRYQDGEHQGV
                     YPAMLATVTAPDGRAVSLHRTYLQDGRKAPVGTPKKLMQGLPLSGAAIRLTAVSEVLG
                     IAEGIETALAASELFEVPVWSCVSAQGIESFEPPAGVKEVVVFADNDTNFAGQAAAYR
                     AAHRLKLKGFEVEVCIPQEPGDWLDHLNKAAERSKGAAK"
     gene            complement(961..2541)
                     /locus_tag="PKX01_16895"
     CDS             complement(961..2541)
                     /locus_tag="PKX01_16895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ77864.1"
                     /translation="MMQCTQAQASAALAGAGVISLRDYQEGLVMEIRDSLKAGRRRVL
                     AYLPTGGGKTRVATAITQMTLSKSKGRVVVLANRKQLVHQFAAALRAAGLDVGVLQGE
                     NTHGLHRRVIVASVDTVHARGCIFDDMALFIIDEAHACAGSEKYRALLFRYSRVPCIG
                     LSATPFARGLGKPYPELADRPLFEDLVIGATVQSLVDAGHLTDLEIYAPSSPDMTGAK
                     TSRTAEGEQDYRQADIEEAADRPELVGDILRHWFKLAGGVKTICFASSIAHSQHIVEQ
                     FRAAGVSAEHLDCYMDDETRADILGRFERGDFTVLSNVSLLSEGFDVPDTSCMILARP
                     TKSLTRFLQMVGRVLRPAPGKTRALLLDHSGSVERIGHPFDDLPLELDDGNANKAGSR
                     KEERKKSEPKPCPSCKFVRPAGVHECPKCGFAPQRQSDVEVADGELVKIDRKARKPAT
                     PDVKQHVYSQLLHVARHRGYKPGWAANQYRARFGVWPRGLRDVEAAPSAELLGWLKSQ
                     AIRFAKAREKAQEGRQHG"
     gene            complement(2538..3092)
                     /locus_tag="PKX01_16900"
     CDS             complement(2538..3092)
                     /locus_tag="PKX01_16900"
                     /inference="COORDINATES: protein motif:HMM:NF018785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HMZ77865.1"
                     /translation="MAAKITPPVRLMLDIEAWKDETQLMTAAERGALLALKMFFWRTG
                     PLPDDDAVLARIVGMEVKDWRKTRKALEPQFIVKCGEWLREDWNTELEEAYAAVNRAK
                     ELSRRGNAARWGRRREPAESDPAGSPAGSPAGSPARDPKYIQRAQRAETPPKTPKPRA
                     NDVQPEFMGDVDLAERALGIGGAA"
     gene            complement(3092..3478)
                     /locus_tag="PKX01_16905"
     CDS             complement(3092..3478)
                     /locus_tag="PKX01_16905"
                     /inference="COORDINATES: protein motif:HMM:NF025456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ77866.1"
                     /translation="MSASRRDNARGQAGEVGKANGYQRQDSTARDLFLESLPEPVAAN
                     HPHVSTAGEAQRQRIVEMLQTGPKHTLDFRRAGIMQSQTRIFELRALGYDIPTVSRVT
                     IYDDQGFPHRGVAVYELVAEPAQGVL"
BASE COUNT          542 a         1196 c         1133 g          628 t
ORIGIN      
        1 gccacgccga agcttcatct tcattttgcc gcccctttgc tgcgctcggc agccttatta
       61 agatggtcca gccagtcgcc cggctcctgc ggaatgcaca cctcaacctc gaaacccttg
      121 agcttgaggc gatgggcggc ccggtaagcg gcggcctgcc cggcgaagtt ggtgtcgttg
      181 tcggcgaaca cgaccacctc tttgacgccg gccggcggct cgaagctctc gatgccttgg
      241 gccgatacgc agctccacac cggcacctcg aacagctccg atgctgcaag cgcagtctcg
      301 atgccctcgg cgatgcccag cacctcggaa acggccgtca ggcgaattgc tgcgccggat
      361 aggggcaacc cctgcatgag cttcttcggc gtgcccacgg gcgctttgcg gccgtcctgg
      421 aggtatgtcc ggtgcagact cacggcgcgg ccatccggcg cggtcacggt cgccagcatc
      481 gcgggataga cgccctggtg ctcgccgtcc tgataccgca ggccgggatg cagccggacg
      541 gcttccggca ggtcgtacag gcgcagccct cgcccggcca ggtaggcata ggcttcgtcg
      601 ccgcgctgaa ttggcttcga ctcccgccac acccggcgca gggcggcgag cttgtcatcg
      661 tcggtgcggt cgggcttgcc ggtcacgggc tgcaccacac ccgccagccg ttcgatttcg
      721 cgggcggcgt ccttgaacga caggccggtg atgcccatcg ccagccgcac gccgtcaccc
      781 gccccgcagc cgctgcaaaa gtacgtccct cgcccatcct tgtcatcgaa cctgaagcga
      841 tccctgccgc cacacagcgg gcagggaccg tgcttgccgc tgagggctcg ctcatccacg
      901 cccaggctca ggaggatggc tcgccagcgg ccggcggcgg cggtgcgaac atcgatacgg
      961 tcacccatgc tggcggccct cctgcgcctt ctcgcgggcc ttggcgaagc ggattgcctg
     1021 cgacttcagc cagcccagca gctcggcact cggcgcggcc tccacatcgc gcaggccacg
     1081 cggccaaacc ccgaagcggg cgcggtactg gttggcagcc cagccgggct tgtagccacg
     1141 gtgccgggcc acatgcagca gttgggaata gacgtgctgc ttcacgtcgg gcgtcgctgg
     1201 cttccgggcc ttgcggtcga tcttcacgag ctcgccgtcg gccacctcca catctgactg
     1261 gcgctgcggc gcgaatccgc acttggggca ctcatgcacg ccagccgggc ggacgaactt
     1321 gcagctcggg cagggcttcg gctcggactt cttgcgctcc tccttccggc tgccggcctt
     1381 gttggcgttg ccgtcgtcca gttcgagcgg caggtcgtcg aaggggtgcc cgatgcgctc
     1441 cacgctgccg gagtgatcca gcagcaaggc gcgggtcttg ccgggcgccg ggcgaaggac
     1501 acggccgacc atctgcaaga accgggtgag gctcttcgtc ggcctcgcca agatcatgca
     1561 gctcgtgtcc ggcacgtcga agccttcgga cagcagggag acgttcgaca acacggtgaa
     1621 gtcaccgcgc tcgaagcgcc ccaggatgtc ggcgcgggtc tcgtcgtcca tgtagcagtc
     1681 gaggtgctcg gcactcacgc cagcggcgcg gaactgctcg acgatgtgct gggagtgcgc
     1741 gatggacgat gcaaagcaga tggttttgac gcctccggcg agcttgaacc agtgccgcag
     1801 gatgtcgccc accagctccg gccggtcggc ggcctcttca atgtcggcct gccgatagtc
     1861 ctgctcgccc tcggcggtgc gagaggtttt cgcgccggtc atgtccgggc tgctcggtgc
     1921 gtagatctcg aggtccgtca gatgccccgc atccaccaac gattgcaccg tcgcgccaat
     1981 caccaaatcc tcgaacagcg gcctatctgc cagctcgggg tacggcttgc ccagccctcg
     2041 ggcaaagggc gttgcgctca ggccgatgca gggcacgcgg ctgtagcgga acagcaaggc
     2101 gcggtacttc tcgctcccgg cgcaggcgtg cgcctcgtcg atgatgaaca gggccatgtc
     2161 atcgaagatg cagccgcggg catggacggt atcgacgctg gcgacgatca cgcggcggtg
     2221 caggccgtgt gtgttctcgc cctggaggac gcccacatcc agcccggcgg cccggagcgc
     2281 ggcggcgaac tgatgcacca gctgcttccg gttcgccagc acgacaacgc ggcccttgga
     2341 cttgctcagg gtcatttgcg tgatggccgt cgcaacgcgg gtcttgcccc cgccggtcgg
     2401 caggtaggcc aggacgcggc ggcgcccggc cttgagcgag tcgcggatct ccatcaccag
     2461 accttcctga tagtcccgca ggctgataac cccggcccca gccagggcgg cggatgcttg
     2521 ggcttgggtg cattgcatca tgcagcaccc ccgataccga gggcgcgctc ggcgagatcc
     2581 acgtccccca taaactccgg ctgaacatca ttcgcccttg gcttgggcgt tttgggcggg
     2641 gtttctgcgc gttgggcacg ctgtatatat ttaggatctc gtgcgggact ccctgcggga
     2701 ctccctgcgg gactccctgc gggatcgctt tcggcaggct ctcggcgcct cccccaacgg
     2761 gccgcgttgc ccctccggct cagctccttg gcccgattga cggcggcgta agcctcctcc
     2821 aactcggtgt tccagtcctc gcgcagccat tcgccgcact tcacgatgaa ctgcggttcc
     2881 agggctttgc gggtcttccg ccagtccttc acctccatgc cgacgatgcg agccagaaca
     2941 gcatcgtcat cgggcagcgg gccggtgcgc cagaagaaca tcttcagcgc cagcaaggcg
     3001 ccgcgctcgg cggcggtcat aagctgtgtt tcgtccttcc acgcctcgat atccaacata
     3061 aggcgcaccg gcggcgtgat cttggcggcc atcacagcac cccctgagcc ggttcggcga
     3121 ccagctcata gacggccacg ccacgatgtg gaaagccttg gtcgtcgtaa atcgtcaccc
     3181 gcgaaacggt cggaatgtcg tagcccagcg cacgcagctc aaagatgcga gtttgcgact
     3241 gcatgatgcc ggcacgccgg aagtcgagtg tgtgcttcgg gcctgtctgc agcatctcta
     3301 caatgcgctg acgctgggct tcgccggcgg tgctgacgtg cgggtgattg gcagctacag
     3361 gttcggggag gctttccagg aagagatccc tggcggtaga atcttgtcgc tgatagccgt
     3421 tagctttgcc gacttcgccc gcttggccgc gggcgttgtc acgtctggag gcggacattt
     3481 aggccacctc gccattcag
//
LOCUS       DAOQPA010000209         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_19079_length_3490_cov_13.404076, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000209 DAOQPA010000000
VERSION     DAOQPA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19079_length_3490_cov_13.404076"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="PKX01_16910"
     CDS             <1..1767
                     /locus_tag="PKX01_16910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ77867.1"
                     /translation="PAAATTKPAAPPTPTSPAQAGTAAAVGAPVTDPNAPQTPPPPPL
                     SDVIFTNGDVITLDDKRPTAQAVAIKGSKILAVGKTKDILARWKSEGTEVVDLAGKTL
                     APGFIDAWGGLSRLGLNSVAAQIQPPPAGTAMDVAAIQRTLRDWAKGDIARRFGWIVG
                     FGYDDSLLREQRPLSRADLDAVARDTPVLIIHASGRQVVLNSRALELAGITRDSTAPA
                     GGAIGRWPGTKEPDGVLDGAAAVALLGQLPQLPPDERQALIQQGQTLYLRNGYTTAVE
                     ARATPEDLALYTQAADTGALKLDVAIYADLAASGAVLKGHKSVGPRYYKNRLRLAGVS
                     MALDGSAEDRSAWLTDAYRLPPPGKRAGFAGYGFATDEDVTALATRATANGWALAVQA
                     NGDAAIDQFLRALRAATPAPGKGLRPLLAGAQTLRDDQLDALQVLGVGVSLAPQRLAL
                     SRPVLVDDTLGTERTAHFAPAAGVLARGLPLTLHTDPALIEPAPLAVMAAALKRPVGA
                     DQQIAAVDAFKALTLTAARQIGEDKTKGSVVAGKLADLVVLSANPLKTPPERLGDIQV
                     IGTVKEGVTVFGGADGGVPITR"
     gene            complement(1959..2627)
                     /locus_tag="PKX01_16915"
     CDS             complement(1959..2627)
                     /locus_tag="PKX01_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ77868.1"
                     /translation="MSTVNAASWGARACLITLGLLLGTVAQSVSAQTLPPSPRDHLER
                     IVGGKPGERKAAEDAGRKASFFCANCHGETGNSKYSEVPNLASQHPAYVLNQIEAFLS
                     GKRKDAFMQGLMKVLSAEEKADIAVYFAAQTVIPSARPGARAAEGRTLFQQNCARCHG
                     PEAKGGETFPRLAGQQPEYLRRSLTRYLKQTGERIYPPMTAAVTGLGERNIDAVVDFL
                     SSLN"
     gene            2763..>3490
                     /locus_tag="PKX01_16920"
     CDS             2763..>3490
                     /locus_tag="PKX01_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HMZ77869.1"
                     /translation="MSPMSAPPAPPSRPCVAIVGGGPAGLMAAETLARGGVAVEVFDA
                     MPSVGRKFLLAGRGGLNITHSEPPERFLTRYGSRQGQIAPLLDAFGPEALRAWVEALG
                     IATFTGSSGRVFPEGMKAAPLLRAWLQRLREAGVRVHMRHRWTGWDADGALRFATPEG
                     ERHVRADAVVLALGGASWARLGSDGAWVPLLAARGADVAPLRPANCGFVAASPRAADG
                     RGWSEHFRSRHAGEPLKGVAMGVAG"
BASE COUNT          465 a         1262 c         1290 g          473 t
ORIGIN      
        1 cccgccgcgg ccacgaccaa acccgcagcg ccgccgacgc cgacgtcccc cgcgcaggcc
       61 ggcacggccg ctgctgtggg cgcgcccgta accgatccca acgcgccgca gacgccgcct
      121 ccgccgcccc tgtcggacgt gatcttcacc aacggcgacg tgatcaccct ggacgacaag
      181 cgccccacgg ctcaggcggt ggcgatcaag ggcagcaaga tcctggccgt gggcaagacc
      241 aaggacatcc tggccaggtg gaagagcgag ggcaccgagg tggtggacct ggcgggcaag
      301 accctggcgc cgggcttcat cgacgcctgg ggcgggctgt cgcgcctggg gctcaattct
      361 gtggcggccc agatccagcc gccgccggcc ggcacggcga tggacgtggc ggccatccag
      421 cgcacccttc gggactgggc caagggcgac atcgcccggc gcttcggctg gatcgtcggc
      481 ttcggctacg acgacagcct gctgcgcgaa cagcgtcccc tgtcacgggc ggatctggac
      541 gccgtcgccc gcgacacgcc ggtgctgatc atccacgcct ccggccgcca ggtggtgctc
      601 aacagccgcg cgctggagct ggccggcatc acccgcgaca gcaccgcgcc ggcaggtggg
      661 gccatcggcc gctggccggg caccaaggag ccggacgggg tgctggacgg cgctgccgcg
      721 gtggcgctat tggggcagct gccgcagctg ccgccggacg aacgccaggc cctgatccag
      781 caaggccaga ccctgtatct gcgcaacggc tacaccaccg ccgtggaagc ccgcgccacg
      841 ccggaggatc tggccctcta cacccaggcc gccgacaccg gcgccctcaa gctggacgtg
      901 gcgatctatg ccgatctggc cgcgagcggc gccgtgctca agggccacaa gagtgtgggg
      961 cccaggtact acaagaaccg cctgcgcctg gccggcgtgt ccatggccct ggacggcagc
     1021 gccgaggacc gcagcgcctg gctcacggac gcctaccgcc tgcccccgcc gggcaaacgc
     1081 gccggctttg cgggctacgg cttcgccacc gacgaggacg tcacggccct cgcgacccgc
     1141 gccacggcca acggctgggc gctggcggtg caggccaacg gcgacgcggc catcgaccag
     1201 ttcctgcgcg ccctgcgggc cgccactccg gcgccgggca agggcctgcg tcccctgctg
     1261 gctggcgccc agacgctccg cgacgatcag ctcgacgcgc tccaggtact gggcgtcggc
     1321 gtgagcctgg cgccccagcg cctcgccctg tcccgtccgg tgctggtcga cgacaccctc
     1381 ggcacggagc gcaccgcgca cttcgccccc gcggccggcg tgctcgcccg cggcctgccc
     1441 ctgaccctgc acaccgaccc ggccctgatc gaaccggcgc cgctggcggt gatggccgcc
     1501 gcgctgaagc gcccagtcgg cgcggaccag cagatcgccg ccgtggacgc cttcaaggcc
     1561 ctgaccctca ccgccgcgcg gcagatcggc gaggacaaga ccaagggcag cgtggtggcc
     1621 ggcaagctgg cggatctggt ggtgctctcc gccaatcccc tcaagacgcc gccggaacgc
     1681 ctgggcgaca tccaggtgat cggcacggtg aaggaggggg tgacggtctt cggcggcgcg
     1741 gacggcgggg tgccgatcac acgctgaggc gggaatggat gcgacgcggc cgaccggtga
     1801 cggcgggatc ttcgcgcagg tacggaagcg gcgcggacgg ccgccagcgg ggggctgggg
     1861 ggcgcagcgc acctgcccga ccacctgtgc gggacgatga ccgcgatcgg tcgtcccgac
     1921 gcgggcccgg ccggatcgtc cgtgccggac ccggacgctc agttgaggct ggacaggaaa
     1981 tccaccaccg cgtcgatgtt gcgctcgccc aggccggtga cggcggcggt catgggcggg
     2041 tagatacgct cgccggtctg cttgaggtag cgggtcaggc tgcgccgcag gtattccggc
     2101 tgctggccgg ccaggcgcgg gaaggtctcg ccgcccttgg cctcggggcc gtggcagcgg
     2161 gcgcagttct gctggaagag ggtgcgccct tcggccgcgc gggcgccggg ccgggcagag
     2221 gggatcaccg tctgagcggc gaaatagacg gcgatgtcgg ccttctcctc ggcgctcagc
     2281 accttcatca ggccctgcat gaaggcatcc ttgcgcttgc cggagaggaa ggcctcaatc
     2341 tggttgagca cgtaggccgg gtgctggctg gccaggttgg gtacttcgga atacttgctg
     2401 ttgccggtct cgccgtgaca gttggcgcag aagaaggagg ccttgcgccc ggcatcctcg
     2461 gcggccttgc gctcgccggg cttgccaccg acgatgcgct ccaggtgatc ccgcgggctg
     2521 ggcggcaggg tctgggcgct gaccgattgg gcgacggtgc ccagcagcag tcccagcgtg
     2581 atcaggcagg cacgggcgcc ccaggacgcc gcgttgacgg tggacatgat gtctcctcgt
     2641 tggacgtgtc gggacggacg accctgtccg ctccgagtga agcggcattg tagtcgcgcc
     2701 gcggccgtcc cgcacggcat gcgcgggacg atccactaca attccggttc cgtcctgccc
     2761 ccatgtcacc gatgtccgct ccgcctgctc cgccctcccg cccctgcgtc gccatcgtcg
     2821 gcggcggccc tgccggcctg atggcggccg agaccctggc ccgcggcggt gtggcggtgg
     2881 aggtcttcga cgccatgccc tcggtggggc gcaagttcct gctggccggg cgcggcgggc
     2941 tgaacatcac ccattccgaa ccgccggaac gcttcctcac ccgctacggt tcgcgccagg
     3001 ggcagatcgc gccgctgctc gacgccttcg gtccggaagc gttgcgggcc tgggtggagg
     3061 cgctggggat tgccaccttc accggcagct cgggccgcgt ctttcccgag ggcatgaagg
     3121 ccgcgccgct gctgcgcgcc tggctgcagc gcctgcgcga ggccggcgtg cgggtgcaca
     3181 tgcgccaccg ctggaccggc tgggacgcgg acggcgccct gcgctttgcc acaccggagg
     3241 gcgagcgcca cgtccgtgcc gacgccgtgg tgctggctct gggcggcgcg agctgggcgc
     3301 ggctgggttc ggacggcgcg tgggtgccac tgctggcggc gcggggggcg gacgtggcgc
     3361 ccttgcgtcc ggccaactgc ggctttgtcg ccgcctcgcc gcgggcggcg gacgggcgcg
     3421 gctggagcga acatttccgc agccgccatg cgggcgagcc cctcaagggc gtggcgatgg
     3481 gcgtggccgg
//
LOCUS       DAOQPA010000210         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_19511_length_3446_cov_14.351814, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000210 DAOQPA010000000
VERSION     DAOQPA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19511_length_3446_cov_14.351814"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKX01_16925"
     CDS             <1..592
                     /locus_tag="PKX01_16925"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77870.1"
                     /translation="STAWCWSNPCRPVSAPLDGVVLEQSVQTGQRVEPAAPIFRIARL
                     SPLWLEIQAPLEVAAGLREGAPVRLADGALRGRLIAIGRAVDATSQTVLLRAEVGAGA
                     ERLRPGQVVDVELDAPAGGPRRLPAAAVVRHEGASLVFVQTGSDTHGTRFEPRPVKIV
                     AEGGEGLGVDGVAATERVVVKGASGLKALLAGVGRE"
     gene            589..>3446
                     /locus_tag="PKX01_16930"
     CDS             589..>3446
                     /locus_tag="PKX01_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ77871.1"
                     /translation="MILDALIRFALTQRLLLLVATALLAAAGVDAYLNLPIDAFPDVS
                     STQVKIILKAPGMTPEEVETRIAVPVEQEMLGIPRQRLLRSMSKYALTDITIDFEDGT
                     DIYWARQQVGERLANALGNLPPGVSGGMAPITTPLGEMFMFTVDGELPLAERRALLDW
                     VIRPQLRTIPGVADVNSLGGEVRSFEVVPRPQSMAARGLALKDLQAVLEANNRNDGAG
                     RLNDGEEGLLVRAEGAIQTLDDLRAIVVQARDGRVTRVADVAEVRWGALTRYGAVSAD
                     GRGEAVEGMVLGLRGADAKQVVAGVKARLAEIEPTLPEGVHLTVLYDRSRLVERAVGT
                     VSKALGEAIVLVVLLLLAFLGNLRAALVVALMLPLAALSTFILMRFDGLSANLMSLGG
                     LAIAIGMLVDAAVVVVENVENQLAEAQANLPTLHVIYRATREVAVPVASGIAIIIIVF
                     LPLLTLQGLEGKMFAPVALTVVFALFSSLLLSLTVVPVLASWLIRRGAHHEPWLVRVL
                     TAAYTRLLDRALARGRQFVLIAVAGLAAAAVAFTFLGKSFMPTMDEGDLILQLEKLPS
                     IGLDQSLAVDQRLQQALLARVPEIRAIVARTGSDELGLDPMGLNQTDTFLVLTPRDTW
                     RHADPAWLADRVREVMADFPGIGYSFTQPIDMRVSEMLTGVRGDVAIKVFGPDLATLN
                     HLAKAIETAVRAVPGAEDTFTLKNDGVQYLKVSVDRLAAGRFGLNVDDIQNDLKALLE
                     GRQVGVVVEHGRRLPVLLRGPDALRDSPADFAALRLSLPDGGSVPLADVARLTRIDGP
                     VKVDRENAQRYVVIQSNVRDRDLVGFVEDARAAVAAAVPLPAGYRLVWGGQFENQQRA
                     AARLMVVVPVALVLIFLLLFSTFRSVRQALLVLSNIPFALIGGVFGLLLAGEYLSVPA
                     SVGFIALLGVAVLNGVVMVSHFNQLLERGLPLAEVVREG"
BASE COUNT          429 a         1310 c         1193 g          514 t
ORIGIN      
        1 ctcgacggcg tggtgctgga gcaatccgtg cagaccggtc agcgcgcccc tcgacggcgt
       61 ggtgctggag caatccgtgc agaccggtca gcgcgtcgag ccggccgcgc cgatcttccg
      121 catcgcccgc ctgtcgcccc tgtggctgga gatccaggcg cccctggagg tggccgccgg
      181 gctgcgcgag ggcgcgcccg tgcgcctcgc cgacggcgcc ctgcggggcc gcctgatcgc
      241 catcgggcgg gccgtggatg ccaccagcca gaccgtgctg ctgcgcgccg aggtgggcgc
      301 cggggccgag cgcctgcgcc ccggccaggt ggtggacgtg gaactggacg cccccgccgg
      361 cggcccccgc cgcctgcctg ccgccgccgt ggtgcgccac gagggcgcca gcctggtgtt
      421 cgtgcagacc ggcagcgaca cccacggcac ccgcttcgag ccccgcccgg tgaagatcgt
      481 cgccgaaggc ggcgaaggcc tcggcgtgga cggtgtggcc gccaccgagc gggtggtggt
      541 gaagggcgcc tccggcctca aggccctgct cgccggagtc ggccgggaat gatcctcgac
      601 gccctgatcc gcttcgccct gacccagcgc ctgctcctgc tggtggccac cgccctgctc
      661 gccgcggccg gggtggacgc ttacctgaac ctgcccatcg acgcctttcc cgacgtctcc
      721 agcacccagg tcaagatcat cctgaaggcg ccgggcatga cccccgagga ggtcgagacc
      781 cgcatcgccg tgcccgtgga gcaggagatg ctgggcatcc cgcgccagcg cctgctgcgc
      841 tccatgtcca agtacgccct caccgacatc accatcgact tcgaggacgg caccgacatc
      901 tactgggccc gccagcaggt gggcgaacgc ctcgccaatg ccctgggcaa cctgccgccc
      961 ggcgtcagcg gcggcatggc gccgatcacc acgcccctgg gcgagatgtt catgttcacc
     1021 gtggacggcg agctgcccct ggcggaacgg cgcgccctgc tcgactgggt gatccgcccg
     1081 cagctgcgca cgatccccgg cgtggccgac gtgaacagcc tgggcggcga ggtgcgcagc
     1141 ttcgaggtgg tgccgcggcc ccagtccatg gcggcgcggg gcctcgccct caaggacctg
     1201 caggcggtgc tggaggccaa caaccgcaac gacggcgccg gccgcctcaa cgacggggag
     1261 gaaggcctgc tggtgcgcgc cgaaggggcg atccagaccc tcgacgacct gcgcgccatc
     1321 gtcgtgcagg cccgcgacgg ccgcgtgacg cgggtggccg acgtggccga ggtgcgctgg
     1381 ggcgccctca cccgctacgg cgcggtgagc gccgacggcc gcggcgaggc cgtggagggc
     1441 atggtgctgg gcctgcgcgg cgccgacgcc aagcaggtgg tggcgggtgt caaggcgcgc
     1501 ctcgccgaga tcgaaccgac cctgcccgag ggcgtgcacc tcaccgtgct ctacgaccgc
     1561 agccgcctcg tggagcgcgc cgtgggcacc gtctccaagg ccctgggcga agccatcgtg
     1621 ctggtggtgc tcctgctgct ggccttcctc ggcaacctgc gcgccgccct ggtggtggcc
     1681 ctgatgctgc ccctggcggc cctgtccacc ttcatcctga tgcgcttcga cggcctgtcc
     1741 gccaacctca tgagcctggg cggcctggcc atcgccatcg gcatgctggt ggatgcggcg
     1801 gtggtggtgg tggagaacgt ggagaaccag ctcgccgaag cccaggccaa cctgcccacc
     1861 ttgcacgtca tctaccgcgc cacgcgggag gtggcggtgc cggtggcgtc gggcatcgcc
     1921 atcatcatca tcgtcttcct gcctctcctc accctgcagg ggctggaggg caagatgttc
     1981 gcgccggtgg ccctgacggt ggtgttcgcc ctgttctcgt ccctgctgct gtccctcacc
     2041 gtggtgccgg tgctggcctc ctggctgatc cgccgcggcg cccatcacga accctggctg
     2101 gtgcgcgtgc tgaccgccgc ctacacgcgc ctgctcgacc gcgccctggc ccgcggccgc
     2161 cagttcgtgc tgatcgccgt ggccgggctg gccgccgccg cggtggcctt caccttcctc
     2221 ggcaagagct tcatgcccac catggacgag ggcgacctga tcctgcagct ggaaaagctc
     2281 ccctccatcg gcctcgacca gagtctggcg gtggaccagc gcctgcagca ggccctgctg
     2341 gcgcgggtgc cggagatccg cgccatcgtc gcccgcaccg gctcggacga gctgggcctc
     2401 gaccccatgg gcctcaacca gaccgacacc ttcctcgtcc tcaccccccg cgacacctgg
     2461 cgccacgccg acccggcgtg gctggccgac cgggtgcgcg aggtgatggc cgacttcccc
     2521 ggcatcggct actccttcac ccagcccatc gacatgcgcg tctccgagat gctcaccggc
     2581 gtgcgcggcg acgtggccat caaggtcttc gggccggacc tggcgacgct caatcacctc
     2641 gccaaggcca tcgagaccgc cgtccgcgcg gtgcccggcg ccgaggacac cttcaccctc
     2701 aagaacgacg gcgtgcagta cctgaaggtg agcgtggacc gcctcgccgc cgggcgcttc
     2761 gggctcaacg tggacgacat ccagaacgac ctcaaggccc tgctggaagg ccgccaggtg
     2821 ggcgtggtgg tggagcacgg ccggcgcctg ccggtgctgc tgcgcggccc cgacgccctg
     2881 cgcgactccc ccgccgactt cgccgccctg cgcctgtccc tgcccgacgg cggcagcgtg
     2941 cccctcgccg acgtggcccg cctcacccgc atcgacggcc cggtcaaggt ggaccgggaa
     3001 aacgcccagc gctacgtggt gatccagtcc aatgtgcgcg accgggacct ggtgggcttc
     3061 gtcgaggacg cccgcgccgc cgtggccgcc gccgtgcccc tgccggccgg ctaccgcctg
     3121 gtgtggggcg gccagttcga gaaccagcag cgcgccgccg cccgcctgat ggtggtggtg
     3181 ccggtcgccc tggtgctgat cttcctgctc ctgttctcca ccttccgctc ggtgcgccag
     3241 gccctgctgg tcctgtccaa catccccttc gccctgatcg gcggcgtgtt cggcctgctc
     3301 ctggccggcg aatacctgtc ggtgcccgcc tcggtcggct tcatcgccct gctcggcgtg
     3361 gcggtgctca acggcgtggt gatggtgagc cacttcaacc agctgctgga gcgcggcctg
     3421 cccctcgccg aggtggtgcg cgaagg
//
LOCUS       DAOQPA010000211         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_19966_length_3404_cov_15.680502, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000211 DAOQPA010000000
VERSION     DAOQPA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_3404_cov_15.680502"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1727
                     /locus_tag="PKX01_16935"
     CDS             117..1727
                     /locus_tag="PKX01_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ77872.1"
                     /translation="MNVQEFDYVIVGGGSAGCALAGRLSEDPGTSVCVLEAGGEDHSV
                     FVRAPLGIIVMVPFGLMNSWHYFTTPQKGFNGRKGFQPRGKVLGGSSSINAMVYTRCN
                     RNDYDGWAAAGNPGWSYADLLPLFKRAENSHCFSNNEYHGNSGPLHVSYLRSPSPANE
                     AFRAACVEKGIPLNPDYNGATQFGISPTQATQYNGERWNAARAYLHANADRRNLTVLK
                     GCHVRKVVFEGKTATGVELRRDGRVEQIRARKEVILSAGAFGSPQLLMLSGIGPAAHL
                     QQHGIAVLHDLPGVGQNLQDHVTSNLIYRTPRWKDTVGISVRGAWEIIKAIFQWRKER
                     KGWITTCVSESNGFISTCGNNDHPDIQLAFLPSIVDDHTRKTHLGHGYTLHTTLMRPK
                     SRGSVTLQSADPHQPPAIDPGFYTHPDDMAVAIKGARMGFEILEAKAMAAYRGKRLHA
                     VDVNDDKAMEAFLRATCDTEYHPVGTCKMGPDSDPMAVVDAQLRVKGVRGLRVVDASI
                     MPTLTSGNTNAPSMVIGEKAADLIRSGV"
     gene            1763..3298
                     /locus_tag="PKX01_16940"
     CDS             1763..3298
                     /locus_tag="PKX01_16940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HMZ77873.1"
                     /translation="MSTIRLNPLDTAWIFTESRATPNHVGGLLQFRLPEDAPRDFMRQ
                     MMAEFRNHRTFTPPWNRRLKYAFNKNPAPVWIEDDDIDLEYHVRHAALPWPGGERELG
                     ELVGRLQSTPLDLMRPPWECTLIEGLEGNRFAMFIKMHHSLIDGISGMKLLQRAMSTD
                     RQRSLGLPPFWASGSPPASRAGREQALMPTMAHAAEAAVAALSGQARSLPQLAMAFGK
                     ILKRVGDPGDGMVVPFVDSPLSVLNGRVREKRRFATQQFTMERLRTLATAAGGTLNDV
                     VLAICGGALRRFLLERDELPEQSLTAGIPVSVRPADDEGTGNAITFIIATLGTDITGA
                     AERFQAIRRSVRHAKEHVQSLPRQAMGQYTVLLMAPTIVTLLTGVGGHTRPMFNITIS
                     NVPGPDKPLYFRGAELLATYPASIVTHGQAVNITCQSYTGKMNFGYTGCHTSVPRLQR
                     LAVYTADALAELEAAFLPQAAAKAVARSAPASRKAAAKAKANPAKVAARRPKAAGKTA
                     KAA"
BASE COUNT          606 a         1190 c         1084 g          524 t
ORIGIN      
        1 gacccggcag gtgcgtccgc gggtttggcc tatggccccg ccgcgcgcgg gcgtgacata
       61 cttttcgttt cgcgcccgct tggcggcgcc gaaccgatgc gaagggaaat cgaccgatga
      121 atgttcagga atttgattac gtgatcgtcg gcggcggatc ggccggctgt gccctggccg
      181 gccgcctgag cgaagacccc ggcaccagcg tctgcgtgct cgaggccggc ggcgaggacc
      241 actcggtgtt cgtgcgcgcg ccgctgggca tcatcgtcat ggtgccgttc ggcctgatga
      301 acagctggca ctacttcacc acgccgcaga aggggttcaa cggccgcaag ggatttcagc
      361 cgcgcggcaa ggtgctgggc ggctccagct cgatcaatgc catggtctac acgcggtgca
      421 accgcaacga ctacgacggc tgggcggcgg cggggaaccc cggctggagc tacgccgatc
      481 tgctgccgct gttcaagcgc gccgagaaca gccattgttt ttccaacaac gagtaccacg
      541 gcaacagcgg cccgctgcac gtgagctacc tgcgcagccc ctcgcccgcc aacgaggcat
      601 tccgtgccgc ctgcgtcgaa aaaggcattc cactcaatcc tgactacaac ggggccaccc
      661 agttcggcat ttcgcccacc caggccacgc agtacaacgg cgagcgctgg aacgccgcca
      721 gagcctacct gcacgccaac gccgatcgcc gcaacctcac ggtgctcaag ggttgccacg
      781 tccgcaaggt cgtcttcgaa ggcaagaccg ccaccggcgt ggagctgcgc cgggacggcc
      841 gggtggagca gatccgtgcc cgcaaggaag tgatcctgag cgccggggcc tttggctcgc
      901 cccagttgct catgctgtcg ggtatcggcc cggcagcgca cctgcaacag cacggcattg
      961 ccgtgctgca cgacctgccc ggtgtgggcc agaacctgca ggaccacgtc acctccaacc
     1021 tgatttaccg cacgccgcgg tggaaggaca cggtgggcat ttcggtgcgc ggtgcgtggg
     1081 agatcatcaa ggcgattttt cagtggcgca aagagcgcaa gggctggatc accacctgcg
     1141 tgtccgaatc caacggcttc atctcgacct gcggcaacaa cgaccatccc gatatccagt
     1201 tggcgtttct gcccagcatc gtcgacgacc atacccgcaa gacccatctg ggccacggct
     1261 acacgctgca caccacgctg atgcgcccca agagccgcgg cagtgtcacc ttgcagagcg
     1321 ccgaccccca ccagccaccc gccatcgacc ccggcttcta cacacatccg gacgacatgg
     1381 ccgtcgcgat caagggcgcg cgcatgggat tcgaaatcct ggaggcgaag gccatggcgg
     1441 cctaccgggg caagcgcctg cacgcggtcg atgtgaacga cgacaaggcc atggaggcct
     1501 tcctgcgcgc cacctgcgac accgaatacc accccgtggg aacctgcaag atgggcccgg
     1561 acagcgaccc gatggccgtg gtcgatgcgc agctgcgcgt caagggtgtg cggggcctgc
     1621 gtgtggtcga cgcgtccatc atgcccacgc tgaccagcgg caacaccaat gcgccgagca
     1681 tggtgatcgg cgaaaaggcc gccgacctga tccgttccgg cgtatgatgg ccatccgctc
     1741 ccgaaccgag taggcttgca ccatgagcac catccgactg aaccccctgg acaccgcctg
     1801 gatcttcacc gagagccggg ccacccccaa ccacgtgggc ggcctgctgc agttccgcct
     1861 gcccgaggac gcgccacgcg acttcatgcg ccagatgatg gccgagttcc gcaaccaccg
     1921 cacgttcacg ccgccctgga accggcgtct gaaatacgcc ttcaacaaga acccggcgcc
     1981 ggtgtggatc gaggacgacg acatcgacct cgaataccac gtgcgccacg cggccctgcc
     2041 gtggccgggc ggtgagcgcg agctcggtga actggtgggg cggctccaga gcacgcccct
     2101 ggacctgatg cgcccgccct gggagtgcac gctgatcgag gggctggagg gcaaccgctt
     2161 cgcgatgttc atcaagatgc accacagcct gatcgacggc atcagcggca tgaagctgct
     2221 gcagcgcgcg atgtcgaccg accgccagcg cagcctggga ctgccgcctt tctgggccag
     2281 cggttcacca cctgcgtccc gcgcggggcg ggagcaggct ctgatgccga cgatggccca
     2341 tgccgccgag gccgcggtgg ccgcgctcag cgggcaggcc cgctcgctgc cgcagctggc
     2401 gatggccttc ggcaagattc tcaagcgggt gggcgacccg ggcgatggca tggtggtgcc
     2461 ttttgtcgat tcgcccctgt ccgtcctgaa tgggcgggtg cgcgagaagc ggcgttttgc
     2521 gacgcagcag ttcacgatgg agcgcctgcg cacgctggcc accgcggctg gcggcacgct
     2581 caacgacgtg gtgctggcca tctgtggcgg agccttgcgg cgcttcctgc tggaacgcga
     2641 cgagttgccc gaacagtcgc tgaccgcggg gattccggta tcagtgcggc cggccgacga
     2701 cgagggcacc ggcaacgcga tcaccttcat catcgccacg ctgggcaccg acatcaccgg
     2761 tgccgccgag cgcttccagg ctatccgccg atcggtgcgc cacgccaagg agcacgtgca
     2821 aagcctgccg cgccaggcga tggggcagta caccgtgctg ctgatggcgc ccaccatcgt
     2881 cacgctgctg accggcgtcg gtggccacac gcggccgatg ttcaacatca cgatctccaa
     2941 cgtgccgggc cccgacaagc cgctgtactt ccgcggcgcc gaactgctgg ccacctaccc
     3001 ggcgtccatc gtgacgcatg gccaggcggt caacatcacc tgccagagct acaccggcaa
     3061 gatgaatttc ggctacaccg gctgtcacac ctcggtgccg cgcctgcaac ggctggcggt
     3121 ctacaccgcc gatgcgctgg ccgaactgga agccgccttc ctgccgcagg ccgccgccaa
     3181 ggcggtggcc cgatcggccc cggcgtcgcg caaggccgcc gcaaaggcca aggcaaaccc
     3241 cgccaaagtc gccgcgcgca ggccgaaggc tgcgggcaag acggccaagg ccgcttgata
     3301 cggattcgcc ttcaaacgta taacttcgtt gcttcgcctc gccgtgctac agcactgtct
     3361 tcggcttcgc gccttgttct acgcttgaat gcaaacccgt atga
//
LOCUS       DAOQPA010000212         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_20683_length_3334_cov_13.011284, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000212 DAOQPA010000000
VERSION     DAOQPA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20683_length_3334_cov_13.011284"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKX01_16945"
     CDS             complement(<1..342)
                     /locus_tag="PKX01_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77874.1"
                     /translation="MTADRHALLCHPDAEPPAGVGVAASATRTADGGLQLEYRLRGDL
                     SGLDLPTAETVLPPERLWAYTCLEAFVATAGETAYVEFNFAPNGQWHRFAFSAYRQGV
                     TPPQTPAPQIAL"
     gene            573..1010
                     /locus_tag="PKX01_16950"
     CDS             573..1010
                     /locus_tag="PKX01_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HMZ77875.1"
                     /translation="MSLPPQRRFPDRLRQVALFELGGLLFITPPFAWLSGVPVRESVG
                     LLAVLALIAAVWNGVFNTLFDRLEGHFTGRTADRRPWLLRGLHAVLFEGILLLLTLPV
                     IVLWTGMNWWPALLADIGLALAYTGYAFVFNLGYDRAFPIAPE"
     gene            1105..1956
                     /gene="asd"
                     /locus_tag="PKX01_16955"
     CDS             1105..1956
                     /gene="asd"
                     /locus_tag="PKX01_16955"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586105.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HMZ77876.1"
                     /translation="MSDRLKVLPQYLLPKQALTVLAGTLAGAQAGGLSTAAIRAFIGR
                     YGVNMAEAEQPDPAAYACFNDFFTRALRAGARPLADADFLCPVDGAISQFGPITRDRI
                     FQAKGHDYSTHALVGGDRDLAIQFQDGDFATLYLSPRDYHRIHMPCDGRLTRMIHVPG
                     ALFSVNPTTARGVPGLFARNERVVCVFENDRFGPFVLVLVGATIVGSMATVWHGQVNP
                     PRPGTVREWDYAPGQVELLKGAEMGRFLLGSTVVLLFPKGRIAFTPTWQPGGAIRMGE
                     AMGRSRL"
     gene            1973..3145
                     /locus_tag="PKX01_16960"
     CDS             1973..3145
                     /locus_tag="PKX01_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ77877.1"
                     /translation="MSAPRRVLPQIVVAQLFGTSLWFSANSAGDDLMRAWQLLPADLG
                     HLTNAVQLGFILGTLGFSLTGLADRYPASRIFALCALIGAAANAAFALFADSLGTALV
                     LRFGVGLALAGVYPLGMKLVVSWVPEQAGAALAWLVGMLTLGTALPHGVRMLDTGWGW
                     QATVLVSSGLALVAAGLIARLGDGPHLVRRHGAPPLRLGAVLMSFALPRFRASACGYF
                     GHQWELYAFWTLVPLLAGTLRPGPVAGPAFAVIGIGAVGCVLGGVLSRRIGSARVAAL
                     ALALSAACCAVYPWVARGPEALAAAVMLLWGASVVADSPHFSALSARACPPEIVGSAL
                     AFQNAIGFAITMGSIALTTRLWPVLHEAVAWVLLPGPLLGLAALYPLWRRGADRPA"
BASE COUNT          392 a         1275 c         1139 g          528 t
ORIGIN      
        1 gagggcgatc tgcggcgcgg gcgtctgcgg cggcgtcaca ccctggcggt aggcgctgaa
       61 ggcgaagcgg tgccactgcc cgttgggggc gaagttgaac tccacgtagg ccgtctcgcc
      121 ggccgtggcg acgaaggcct ccaggcaggt gtaggcccac aggcgttcag gcggcagcac
      181 cgtctccgcc gtgggcaggt ccagcccgct cagatcgccg cgcaggcgat attcgagctg
      241 caacccgccg tcggccgtcc gcgtcgccga cgccgccacg cccaccccgg cgggcggttc
      301 ggcatccgga tggcacagca gggcgtggcg gtcagcggtc atgggggctc tcggaacggg
      361 cctgacacgg cccggcgcgc agtgtagccg atcgggccgg ggcgccgggc gatgaggccg
      421 tggccgcatc gcccgcgccc gcacgcgaca acacaaccgc ctgacaggcc cttgaccggg
      481 gatctaccgg taggtaccaa cgaacgccgc cgtgcggaca caccggcacg cgcctgacgt
      541 agactgacgc gcccgcgcac tccgcctcac ccatgtccct cccgccccaa cgccgcttcc
      601 ccgaccgcct ccgccaggtc gccctgttcg agctcggcgg gctgctcttc atcacgccgc
      661 ccttcgcctg gctgtctggc gtgccggtgc gggaatcggt cgggctgctg gcggtgctgg
      721 ccctcatcgc ggcggtgtgg aacggcgtgt tcaacaccct cttcgaccgc ctcgaaggtc
      781 atttcaccgg gcgcactgcc gaccgccggc cgtggctgct gcgcgggctc cacgcggtgc
      841 tcttcgaggg catcctgctg ctcctgaccc tgccggtgat tgtcctgtgg accggcatga
      901 actggtggcc ggcgctgctg gcggacatcg gtctggccct ggcctacacg ggctacgcct
      961 tcgtcttcaa tctgggctac gaccgggcct ttcccatcgc cccggagtga gcgccgcggc
     1021 acccgcgccg gccggtataa tcgccgcccg accccccggt cggcccgcgc ccgtggcgcg
     1081 tccccgcctg ctggagattc cgccgtgtcc gaccgcctca aagtgctgcc ccagtacctg
     1141 ctgcccaagc aggccctcac cgtgctcgcc ggcacactgg cgggcgcaca ggcgggcggg
     1201 ctgagcaccg cggcgatccg cgccttcatc ggccgctacg gggtgaacat ggccgaggcc
     1261 gagcagccgg atccggcggc ctacgcctgc ttcaacgact tcttcacgcg tgccctgcgg
     1321 gccggcgcgc ggcccctcgc cgacgcggat ttcctgtgcc cggtggatgg cgccatcagc
     1381 cagttcggcc cgatcacccg agaccgcatc ttccaggcca agggtcacga ctactccacc
     1441 cacgccctgg tgggcggcga ccgggacctg gcgatccagt tccaggacgg cgacttcgcc
     1501 acgctctacc tcagcccgcg ggactaccac cgcatccaca tgccctgcga cggccgtctg
     1561 acgcggatga tccacgtgcc cggcgcgctg ttctcggtca atccgaccac cgcccgcggc
     1621 gtgcccggcc tgtttgcccg caacgagcgt gtggtgtgtg tcttcgagaa cgaccgcttc
     1681 gggcccttcg tgctggtgtt ggtgggcgcc accatcgtcg gcagcatggc caccgtgtgg
     1741 cacggccagg tcaatccgcc gcggcccggc acggtgcggg aatgggacta cgcgccgggc
     1801 caggtcgagc tgctgaaggg cgccgagatg gggcgcttcc tgctcggctc gacggtcgtc
     1861 ctgctcttcc ccaagggccg catcgccttc acccccacct ggcagccggg cggtgcgatc
     1921 cgcatgggcg aggcgatggg ccgcagccgg ctgtgattcc ggccctcaga ccatgagcgc
     1981 gccgcgccgc gtcctgccgc agatcgtcgt tgcccagctc ttcggcacct ccctgtggtt
     2041 ctcggccaac agcgccggcg acgacctgat gcgcgcctgg cagctgctgc cggcggacct
     2101 gggccacctc accaacgccg tgcagctcgg cttcatcctg ggcaccctgg gcttctccct
     2161 caccgggctg gcggaccgct atcccgccag ccgcatcttc gccctgtgcg ccctgatcgg
     2221 cgccgctgcc aacgccgcct ttgccctctt tgccgacagc ctgggcacgg cactggtcct
     2281 gcgcttcggc gtgggcctgg cgctggccgg ggtctacccg ctgggcatga aactggtggt
     2341 gagctgggtg ccggagcagg ccggcgcggc cctggcctgg ctggtgggca tgctcaccct
     2401 gggcaccgcc ctgccccacg gcgtgcgcat gctcgacacg ggctggggct ggcaggccac
     2461 ggtgctggtt tcgtcgggcc tggccctcgt cgccgccggc ctgatcgccc gcctcggcga
     2521 cgggccgcac ctggtgcgtc ggcacggcgc cccgcccctg cgcctcggcg ccgtgctgat
     2581 gagcttcgcc ctaccccgct tccgcgcctc ggcctgcggc tacttcgggc accagtggga
     2641 gctttacgcc ttctggacgc tggtgccgct gctggccggc acgctgcggc cggggccggt
     2701 ggcgggcccc gccttcgcgg tgatcggcat cggcgccgtc ggctgcgtgc tgggcggcgt
     2761 cctcagccgg cgcatcggca gcgcccgcgt ggcggccctc gcgctggccc tctcggcggc
     2821 ctgctgtgcg gtctatccgt gggtggcccg cggaccggag gcgctggccg cggcggtgat
     2881 gctgctgtgg ggcgccagcg tggtggcgga ttcgccccac ttctcggccc tctccgcgcg
     2941 cgcctgtccg ccggagatcg tgggcagcgc cctggccttc cagaacgcca tcggtttcgc
     3001 catcaccatg ggttccatcg ccctcaccac ccgcctgtgg ccggtgctcc atgaagccgt
     3061 cgcgtgggtg ctgctgcccg gcccgctgct gggcctggcc gccctgtacc ccttgtggcg
     3121 gcggggcgcc gacagaccgg cctgaacgcc gctgcaggcg ccgtcaggcc cccttcgcgg
     3181 agtgccgccg ggtcgcaaaa aagtggttca tcacgccgat tccccagccc aggatcggcc
     3241 accacgccca gtcgagacgc ccgtcgcgct gcacgtccag cacgatgaac agcaacgaca
     3301 tcgcgacgta gattcccaga tggcgggaaa aacg
//
LOCUS       DAOQPA010000213         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_20700_length_3332_cov_18.739701, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000213 DAOQPA010000000
VERSION     DAOQPA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20700_length_3332_cov_18.739701"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1260
                     /locus_tag="PKX01_16965"
     CDS             115..1260
                     /locus_tag="PKX01_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HMZ77878.1"
                     /translation="MGPLHGIKVIEFAALGPCPMAAMILADLGAEVVRIERKPAPGAK
                     PASDLFDPAIDILNRSRRVVTLDLKKPEALAAARQLIAGADVLVEGFRPGVMERLGLG
                     PDETLAANPRLVYGRMTGWGQHGPLAQAAGHDINYLSLSGALHAIGEPGGKPVVPLNL
                     VADCGGGAMLLVVGVLAAVLEARQSGRGQVVDAAMTDGSALLMTMMYTFKAMGQWTQA
                     RGSNLLDGGAPFYDTYRCADGKYLSIGPIEPPFYALFLEKAGLTDPDFRQQWDRARWP
                     ELKQRVAAHLATRPRDAWCALLEGTDACVAPVLDMDEAPAHPHNRARGTFIEVNGVVQ
                     PAPAPRFSRSTPAAPTPPTPGAPGEDVLADWGLSPDAIATLKAVGGL"
     gene            1285..2061
                     /locus_tag="PKX01_16970"
     CDS             1285..2061
                     /locus_tag="PKX01_16970"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HMZ77879.1"
                     /translation="MSYENILSEVRGKVGLITLHRPKALNALNDDLVNEVRQALDAFE
                     ADEGIGCIVITGSEKAFAAGADIGAMASYSYMDAYKGDYITRNWERVKTCRKPVIAAV
                     AGYALGGGCELAMMCDMIFAADTARFGQPEIKLGTLPGAGGTQRLPRAVGKAKAMDLC
                     LTARLMDAAEAEKAGLVARVIPADRLLEETLAAAEQIAGYSLPVVMMIKESINRAYES
                     ALNEGLLFERRLFHASFGLEDQKEGMAAFVEKRKADFKNR"
     gene            complement(2171..2683)
                     /locus_tag="PKX01_16975"
     CDS             complement(2171..2683)
                     /locus_tag="PKX01_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMZ77880.1"
                     /translation="MAVGPMVWSRLVRLTHWGVAAAVLFDLFNETGPLHRATGYGAVA
                     LVLLRLAYGACMPAGDPAALSRPGLTAIGVHLREMGRGHLSPVAGHNPLGQWAAYLMW
                     ALVLALGLTGWMSRLDAFWGDDGLIELHEALADGLMACVALHLMAVALVSRLQGESLL
                     RAMLTGRRRG"
     gene            complement(2697..2957)
                     /locus_tag="PKX01_16980"
     CDS             complement(2697..2957)
                     /locus_tag="PKX01_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HMZ77881.1"
                     /translation="MRALILAILTLSPLAAFAGARCEAHPKSEWMSEENAKARLVAEG
                     YQIRKFKVDGNCYEIYGKNREGKKVEIYFDTKTLAIVKAEIE"
BASE COUNT          584 a         1267 c         1014 g          467 t
ORIGIN      
        1 ggcaacgctg gcgagttccg cgccgggcgc cggggcgaga cccggccaag ccgcgcaaaa
       61 cgggcgccgc cggctcaagc gaaacaagaa caaaaaacca aggagacaac accgatgggc
      121 cccctccacg gcatcaaagt catcgaattc gccgccctcg gcccctgccc gatggccgcc
      181 atgatcctgg ccgacctggg cgccgaggtc gtgcgcatcg agcgcaagcc cgcgccgggc
      241 gccaagccgg cttctgacct gttcgacccg gccatcgaca tcctcaaccg cagccgccgt
      301 gtcgtcaccc tcgacctgaa gaagcccgaa gccctcgccg ccgcccgaca gctcatcgcc
      361 ggcgccgatg tcctcgtgga gggcttccgc cccggcgtga tggaacgcct gggcctgggc
      421 ccggacgaga ccctcgcggc caatccgcgc ctcgtctatg ggcgcatgac cggctggggg
      481 cagcacggcc ccctcgccca ggccgccggt cacgacatca actacctgtc cctgtccggc
      541 gccctgcacg ccatcggcga acccggcggc aagcccgtgg tgcccctcaa cctggtggcg
      601 gactgcggcg gcggcgccat gctgctggtg gtgggcgtcc tcgcggcggt gctggaagcc
      661 cgccagtcgg gccgtggtca ggtggtggac gcggccatga ccgacggctc ggccctgctc
      721 atgaccatga tgtacacctt caaggccatg ggccaatgga cgcaggcgcg gggcagcaac
      781 ctgctggacg gcggcgcccc cttctacgac acctaccgct gcgccgacgg caaatacctg
      841 tccatcggcc ccatcgagcc gcccttctac gccctgttcc tggaaaaagc cggcctcacc
      901 gatccggatt tccggcagca atgggaccgg gcgcgctggc ccgaactcaa gcagcgcgtg
      961 gccgcgcatc tcgccacccg cccgcgggat gcctggtgcg ccctgctgga aggcaccgac
     1021 gcctgcgtcg cgccggtgct ggacatggac gaagcgcccg cccacccgca caatcgggcc
     1081 cgcggcacct tcatcgaggt gaacggcgtc gtccagcccg ccccggcgcc gcgcttcagc
     1141 cgcagcaccc ccgccgcacc cacaccgccc acgccgggcg ctcccggcga ggatgtactc
     1201 gccgactggg gcctgagccc ggacgccatc gccaccctca aggccgtcgg cggcctgtga
     1261 tccgaatcca cccaaggaga acccatgagc tacgaaaaca tcctcagcga agtccgcggc
     1321 aaggtcggcc tcatcaccct ccaccgcccc aaggccctca acgccctcaa cgacgacctg
     1381 gtgaatgaag tccgccaggc cctcgacgcc ttcgaggccg acgagggcat cggctgcatc
     1441 gtcatcaccg gctccgagaa ggccttcgcc gccggcgccg acatcggcgc gatggcgagc
     1501 tactcctaca tggacgccta caagggcgac tacatcaccc gcaactggga acgggtcaag
     1561 acctgccgca agccggtcat cgccgcggtg gccggctacg ccctgggcgg cggctgcgag
     1621 ctggcgatga tgtgcgacat gatcttcgcc gccgacaccg ccaggttcgg ccagcccgag
     1681 atcaagctcg gcaccctgcc cggcgccggc ggcacccagc gcctgccccg tgccgtgggc
     1741 aaggccaagg ccatggacct gtgtctcacg gcccgcctga tggacgccgc cgaagcggaa
     1801 aaggccggcc tggtggcccg cgtcatccct gccgacaggc tgctggagga aacccttgct
     1861 gcagccgagc agatcgccgg ctattccctg ccggtggtca tgatgatcaa ggaatccatc
     1921 aaccgcgcct acgagtccgc cctcaacgaa ggcctgctct tcgagcgccg cctgttccac
     1981 gcgagcttcg gcctggaaga tcagaaggaa ggcatggccg ccttcgtcga aaagcgcaag
     2041 gcggacttca agaaccgctg agcgggacgc gcgtcccgac gccacggggg ctgcggcccc
     2101 cgttttcgtg aacgccgccc accgcggcgg gcggcacacg tcgcgttcgg aaatccggac
     2161 gcctcaccct tcagccacgc cgacgcccgg tgagcatcgc ccgcagcagg ctctccccct
     2221 gcaggcggct caccagcgcc accgccatca ggtgcagggc gacacaggcc atcagcccgt
     2281 ccgccagcgc ctcgtggagt tcgatgagcc cgtcgtcgcc ccagaaggcg tcaagccggc
     2341 tcatccagcc ggtcaagccg agcgccagga ccagcgccca catcaggtag gcggcccact
     2401 gaccgagcgg gttgtgtccc gccacggggc tcaggtgccc acgccccatc tcccgcaggt
     2461 ggacaccgat cgccgtcaag cccggacggg acagggccgc aggatcgccc gccggcatgc
     2521 atgccccgta ggcgaggcgc agcagcacca gggcgacggc cccatatccc gtggcgcgat
     2581 gcaggggccc cgtctcgttg aacagatcga acagcactgc cgccgccacg ccccagtggg
     2641 tcaggcgcac cagtcggctc cacaccatcg gaccgacagc catcgcggcg acccgcttat
     2701 tcgatctcgg ccttcacgat ggcaagggtc ttggtgtcga agtagatctc caccttcttg
     2761 ccttcccggt tcttgccgta gatctcgtag cagttgccgt ccaccttgaa cttgcggatc
     2821 tggtagccct cggccaccag gcgggccttg gcattctcct cggacatcca ttccgacttg
     2881 ggatgcgctt cacagcgggc acccgcgaag gcagcgagcg gggagagggt caggatggca
     2941 aggatcaggg cacgcatgga agctcctatc aggcaatgaa gtgaagtggg ttggcggtca
     3001 gcctacacaa aacgataacg attctcaata ccaaattcac cgtcggattt ccgaacaccc
     3061 cgcggaccgc catccggatt cccgaacagg cacgcccgcg cgcttcagaa atttcatatt
     3121 aaatcatcta cttgccgcac ccgctcggtc accggccgca tggcacgccg tctgcaatac
     3181 aaagcgcgtg ttcagccccc ttcccattcg ccggagacga cccatgcgca ctcgctgttc
     3241 cctcgccgct ccttccctgg ccatcgccct tgcggtggcc gccctggccc agcccgccgc
     3301 cgcccaggaa gtgcccaccc tcgccaagat ca
//
LOCUS       DAOQPA010000214         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_20830_length_3319_cov_14.838235, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000214 DAOQPA010000000
VERSION     DAOQPA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20830_length_3319_cov_14.838235"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKX01_16985"
     CDS             <1..310
                     /locus_tag="PKX01_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77882.1"
                     /translation="LVGGGAELEARVRTEQPAATRKLPSPALGERAGGPYPVEPGDED
                     GTLALEPVFLVDLSLPGHALADVGRRAWVRFDLGYAPLAVQAYRHAAQLFLKHFNPTE
                     "
     gene            472..2301
                     /locus_tag="PKX01_16990"
     CDS             472..2301
                     /locus_tag="PKX01_16990"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ77883.1"
                     /translation="MGRFVRAVRRREGELASAGPAQFDAAVTALRLTLGRDGLGPGGL
                     VEAFAVLSVVCAGVLGVRPYDTQLVAARIVLDNSLAEMATGEGKTLAVALAAATAALA
                     GVPVHVITANDYLVSRDAAALEPLYAALGLRVGAVVQSMGADARRDAYACDVAYCTAK
                     ELVFDYLRDGLERTRDRLRWHVQQLCGDGGTAPVLRGLCMAIVDEADSILIDEARVPL
                     ILSRPVAQVQQDRYLGQSLALARSLEAERDYRLEPGPRSAVLTPAGADRVARAADVLG
                     AVWHNRLHREEVIGLALTALHLYRRDRDYLVRDGAVQIVDEHTGRVAPGRAWSRGLHQ
                     LLELKEGCAPTPPTETLAQITYQRFFPRYLRLGGLSGTLREARAELLALYGLPVRGVP
                     LRGPDRRVTLPSRLFADSAALWSAVAARAASVAGQGRPVLVGTATVADSEALSARLAA
                     AGVDHVVLNARQDGDEARVVAEAGLAGRITVATNMAGRGTDIALAAGCAERGGLHVIV
                     CQLNGARRIDRQLAGRCARQGDPGSVETWLAADMPLLHDALPAALRRGLQRWAPRVPD
                     AALKGLRRLLQGREERLQALQRRRLRARDRYLERGLSFRGVSE"
     gene            2466..>3319
                     /locus_tag="PKX01_16995"
     CDS             2466..>3319
                     /locus_tag="PKX01_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77884.1"
                     /translation="MTSKRKGWRYGVGLCLMAGVETALGGQSFGCLIEPERTAEIGSQ
                     VVGIVESFAVERGAFVSKGQPLAVLQAQTERAAVSLAKVKRDADAEVRAAESALDFAR
                     AGQTRADDLFNRNFISAHARDQARTDAEVAAQKLAQAREHRRIADGELEYAQAQLAQR
                     IIRSPFSGVVTERYLSPGERVEEKPLMKLAQIDPLKVQVVAPVAMYGRIEVGRSVQIT
                     PEMPEGKPVEARVTDVDRVLDPASNTFRVVLRLPNPRMELPAGLRCNADFGATGAAAG
                     GGMTAPRPV"
BASE COUNT          437 a         1151 c         1255 g          476 t
ORIGIN      
        1 cctggtcggc ggcggggcgg agctggaagc ccgcgtgcgc accgaacagc ccgccgcgac
       61 ccgcaagctg ccgagtccgg ccctcgggga gcgggccggc ggcccctatc ccgtcgagcc
      121 cggtgacgag gacggcacgc tggccctgga gccggtgttc ctggtggatc tgagcctgcc
      181 gggccacgcc ctcgccgacg tgggccggcg cgcctgggtg cgcttcgacc tcggctacgc
      241 gccgctggcc gtgcaggcct accgccacgc cgcccagctc ttcctgaaac acttcaaccc
      301 caccgagtga ggggccggcc ggtcgttcgt ccatccatgc gtgccgcgtc cctgcccgtc
      361 cctggccccc tgtggggcag ctatccggaa aggcgcacgc cagcggagtc ggcgcagccc
      421 ttcggacggc gtttggccga tctgggacga cgcctcgctg gagcgcgtgg gatgggtcgt
      481 ttcgtccgcg cagtgcggcg ccgggaaggc gaactggcca gcgccggtcc ggcgcagttc
      541 gacgcggccg tcacggcgct gcggttgacc ctggggcgcg acggtctggg gccgggtggg
      601 ctggtcgaag catttgccgt gttgtccgtg gtctgtgccg gggtcctcgg ggtgaggcct
      661 tacgacaccc agctggtggc ggcccgcatc gtgctcgaca acagtctggc ggagatggcg
      721 accggcgagg gcaagaccct ggcggtggcc ctggcggcgg cgacagctgc gctggccggg
      781 gtgccggtgc acgtgatcac cgccaacgat tacctggtga gccgcgacgc ggctgccctc
      841 gagcccctgt acgccgccct gggcctgcgc gtcggcgccg tcgtccagtc gatgggggcg
      901 gacgcacggc gtgacgccta cgcctgcgac gtcgcctact gtacggccaa ggagcttgtg
      961 ttcgactatc tgcgcgacgg gctcgaacgt acccgcgacc gcctgcgctg gcacgtgcag
     1021 caactgtgtg gcgatggcgg tacagcgccg gtcctgcgcg gtctgtgcat ggccatcgtg
     1081 gacgaggcgg acagcatcct catcgacgag gcacgcgtgc ccctgatcct ctcccggcca
     1141 gtcgcccagg tccagcagga ccgctacctc gggcagtccc tggcgctggc gcgcagcctc
     1201 gaggcggagc gggactaccg cctcgagccg gggccgcgca gcgctgtcct gacgccggcc
     1261 ggtgcggatc gggtggcccg cgccgccgac gtgctgggcg ccgtgtggca caaccgcctg
     1321 catcgggagg aggtgatcgg gctcgccctg accgcgctgc atctctatcg gcgggatcgg
     1381 gactatctgg tccgcgacgg ggccgtgcag atcgtggacg agcacaccgg acgcgtcgcg
     1441 cccggccgcg cctggtcacg gggcctgcac cagcttctgg agctgaagga aggctgtgcg
     1501 cccacgccac cgacggaaac cctggcgcag atcacctatc agcgcttctt tccccgctac
     1561 ctgcgcctgg gcggactgag cggcaccctg cgcgaggcac gcgccgagct gctggccctc
     1621 tacggtctgc ccgtgcgggg cgttccgctg cgggggcccg accggcgagt cacgctgccg
     1681 tcgcggctct ttgccgacag cgccgccctg tggtcggcgg tggcggcgcg ggcggccagt
     1741 gtcgccggcc agggccggcc ggtcctggtc gggaccgcca cggttgcgga ctccgaggcc
     1801 ctctcggcgc gtctggcggc tgcgggcgtg gaccatgtcg tgctgaacgc gcgccaggat
     1861 ggcgatgaag cgcgggtggt ggcggaggcc ggcctggcgg gacgcatcac ggtggccacc
     1921 aacatggccg gacgcggtac ggacatcgcg ctggcggccg gctgtgccga gcgcggcggt
     1981 ctgcacgtga tcgtctgtca gctcaacggg gcgcgccgca tcgaccgcca gctggccggc
     2041 cgctgcgccc ggcagggcga tcccggcagc gtggaaacct ggctggcggc agacatgccc
     2101 ctgctgcacg acgccctgcc ggccgcactg cgacgcggac tgcagcgctg ggcgccgcgg
     2161 gtgcccgacg cggcactgaa gggcctgcgg cgcctgctcc aggggcgcga ggagcgcctc
     2221 caggccctgc aacggcgccg cctgcgcgcc cgtgaccggt atctggagcg gggactgtcc
     2281 ttccgcgggg tctccgagta gcggggagcg tcacccgtcc cggcggattg tgacggaagt
     2341 cctcaattca ggggcatgtc cagccgttaa agccaagcag gatgaaggcc gttgccgccc
     2401 tccgggggca ggctgtggcc ggccgggccg acgcgggccg gtccataacg atcggatgca
     2461 agcatatgac cagcaagcgc aaggggtggc ggtacggggt cggactctgt ctgatggcgg
     2521 gcgtggagac cgccctgggc ggacagtctt tcggctgcct gatcgagccg gagcgtacgg
     2581 ccgagatcgg cagccaggtg gtgggcatcg tcgaatcgtt tgccgtggag cggggcgcct
     2641 tcgtgagcaa ggggcagcca ctggccgtgc tgcaggccca gaccgagcgg gcggcggtgt
     2701 ccctggccaa ggtcaagcgc gatgcggacg ccgaggtgcg ggccgcggaa tcggcgctgg
     2761 acttcgcccg tgccggtcag acccgcgccg acgatctgtt caaccgcaat ttcatctccg
     2821 cccacgcccg cgatcaggcc cggaccgacg ccgaagtggc cgcccagaag ctggcccagg
     2881 cgcgggaaca tcggcgcatt gccgacggcg aactggaata cgcccaggcg cagctggccc
     2941 agcgcatcat ccgcagcccc tttagcggcg tggtcaccga gcgctacctc tcgcccggtg
     3001 agcgggtcga ggagaagccg ttgatgaagc tggcccagat cgatccgctc aaggtccagg
     3061 tggtggcgcc ggtggccatg tacgggcgca tcgaggtggg gcgcagcgtg cagatcaccc
     3121 cggagatgcc cgagggcaag ccggtggaag cgcgcgtgac cgacgtggac cgggttctcg
     3181 atccggccag caataccttc cgggtcgtcc tgcgcctgcc caatccgcgc atggagctgc
     3241 cggccggtct gcgttgcaac gccgatttcg gggcgacggg cgcggcggcg ggcggcggca
     3301 tgacagcccc gcgcccggt
//
LOCUS       DAOQPA010000215         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_20919_length_3312_cov_16.596254, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000215 DAOQPA010000000
VERSION     DAOQPA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20919_length_3312_cov_16.596254"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1467
                     /locus_tag="PKX01_17000"
     CDS             223..1467
                     /locus_tag="PKX01_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ77885.1"
                     /translation="MLRILAVAALAALPVLSAAADGPAFTLSALREAARAGHPVLAAA
                     RAQVEGGRAQVDSAAAYPNPELEVLSGRTRARLPGVNAGTVVSVAVTQRVDVPWQRDA
                     RIDVAGHGLTALEAGRRGVENELLARLDQRFYELLRREAEVRAAREDRQLTEQIRSRV
                     AVRVETGEAPRYELFKADTELLNARKTEDSASLRVEQARAALRALVGAALPARFSVIG
                     SLGDRAAVATLDALRDAVMSGNPDLARLRAEVARAARQLDLERLRRQPELAVKVADER
                     DSELRDTRVGLVVSVPLWDRRRGPVAEAAARLEAARSELSAQELAVQQALEGAWRQYE
                     ITLTQVIALENGILREAEAALKVAEAAYRFGERGILDYLDAQRVFRIARNELIAARYD
                     LQLAVIEIERLRAAQPWSGATP"
     gene            1464..2678
                     /locus_tag="PKX01_17005"
     CDS             1464..2678
                     /locus_tag="PKX01_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ77886.1"
                     /translation="MNLPSSFKTATVPAVLLGLFLTACQDARPPAAPAAAARPSPSSA
                     LGEVPGSDTDAPPADPHVVAVPPRFGVALQVLPVASAALSDTLRVAGKVDFDERRVSR
                     IGATVTGRVVEIQAHLGQTVHAGDTLAIINSTELGQAQIVYLKARAQADLQSRSVERA
                     RQLFAADVIGRAELQRRESELAIATAEQRGAEDQLQVLGMSAGAIRRLGAHGAINSVT
                     PVVSATAGTVVERKVTQGQVVQPSDSLFMVADLSQVWVTAEVPEQQAALVKSGQSVEI
                     EVPALGARLTGKLIYVADTVNPDTRTVTVRSAVDNRARQLKPAMLATMLIQAAPAERL
                     VVPAQAVVRDANTDNVFVEIAPQRFRLTPVRLGPEADGRRAVLEGLKAEQRILVAGAF
                     HLNNERKRKELE"
     gene            2675..>3312
                     /locus_tag="PKX01_17010"
     CDS             2675..>3312
                     /locus_tag="PKX01_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ77887.1"
                     /translation="MIESLVRTALQQRLVVVVVAAILLVFGLNAARKLSVDAFPDVTN
                     VQVQIATEAKGRSPEEVERFVTVPLEIAMTGLPGLEEMRSLNKPGLSLITLVFTDATD
                     VYFARQLVMERLLEVGARMPAGVVPVLGPVSTGLGEVYQYTLDHPEDGDRTLTEKELT
                     ERRIVQDWVVRPLLRSIPGVAEINSQGGYARQYQVLVNPDRLRHYQLTVRDV"
BASE COUNT          450 a         1195 c         1207 g          460 t
ORIGIN      
        1 gccgacgtcc tcgagatgcc ggccctcccg gcgctgctgc cgatcgtggg catcgcgccg
       61 cggcccctgt gggtcgccgt gcatccctgc ctgtccccga gtctcccctt ggcgccgccg
      121 gcgccccctc cccgcctcgt ctgatccggc gcccccgtgg cgccgaccgc aggtgcgcac
      181 gccgggaggg cgtgcgcggt tcgagatccg acgaggacat ccatgctacg aatccttgca
      241 gtggccgccc tggcggccct gccggtcctg tctgcggcgg cagacggacc ggccttcacc
      301 ctgtccgccc tgcgcgaggc ggcgcgggca ggacaccccg tcctggccgc cgcccgcgcc
      361 caggtggagg gcggtcgcgc ccaggtggac agcgccgccg cctaccccaa cccggagctg
      421 gaggtgctct ccggccgtac ccgcgcccgt ctgcccgggg tgaacgccgg caccgtggtg
      481 agcgtggcgg tgacccagcg ggtggacgtc ccgtggcagc gggacgcccg catcgacgtg
      541 gccggacacg gcctgacggc cctggaggcc ggccgccgcg gtgtcgagaa cgagctgctg
      601 gcccgcctgg accagcgctt ctatgaactc ctgcggcgcg aggcggaggt ccgcgccgcc
      661 cgcgaggacc ggcagctcac cgaacagatc cgcagccgcg tggccgtgcg cgtggagacc
      721 ggcgaggcgc cccgctacga gctcttcaag gccgacacgg agctcctcaa tgcgcgcaag
      781 accgaagaca gcgccagcct gcgggtggag caggcccgcg cggccttgcg cgccctggtc
      841 ggtgcggcgc tgccggcccg cttctcggtg atcggcagcc tgggggaccg ggcggcggtg
      901 gccacgctgg acgccttgcg ggacgcggtg atgagcggca acccggacct cgcccgtctg
      961 cgcgccgagg tggcccgtgc agcccgccag ctggatctgg agcgcctgcg ccgccaaccc
     1021 gagctggccg tgaaggtggc ggacgaacgt gacagcgagc tgcgcgacac ccgcgtgggc
     1081 ctggtggtga gcgtgcccct gtgggaccgc cgccggggcc cggtggcgga ggcggcggcc
     1141 cgcctggagg ccgcccgcag cgagctgtct gcccaggagc tggccgtgca gcaggccctg
     1201 gaaggcgcct ggcgccagta cgagatcacc ctcacccagg tcatcgccct ggagaacggc
     1261 atcctgcggg aggccgaagc cgccctgaag gtggccgagg ccgcctaccg cttcggcgag
     1321 cgcggcatcc tcgattacct ggacgcccag cgcgtgttcc gcatcgcccg caacgaactc
     1381 atcgccgccc gttacgacct gcagctcgcc gtcatcgaaa tcgagcgcct gcgggcggcc
     1441 cagccctggt ccggagcgac accatgaacc tgccctcttc cttcaagacc gcgaccgtgc
     1501 cggctgtgct gctcggtctg ttcctgaccg cctgtcagga cgccaggccg cctgccgcgc
     1561 cggccgccgc ggcccgtccc tcgcccagct cggccctggg cgaggttccg ggcagcgaca
     1621 ccgacgcgcc gccggccgac ccccatgtgg tggcggtgcc gccccgattc ggtgtggcgc
     1681 ttcaggtgct gccggtggcg tccgcggccc tctccgacac cctgcgggtg gccggcaagg
     1741 tggactttga cgaacgccgg gtgagccgca tcggcgcgac ggtgaccggc cgcgtggtgg
     1801 agatccaggc gcatctgggc cagaccgtgc atgccggcga taccctggcg atcatcaaca
     1861 gcacggagct ggggcaggcc cagatcgtct atctgaaagc ccgcgcccag gccgacctgc
     1921 agtcacgcag cgtggagcgg gcgcgacagc tgttcgctgc cgacgtgatc ggtcgggccg
     1981 agctgcagcg gcgcgagagc gagctggcca ttgccaccgc ggagcagcgc ggggcggagg
     2041 accagctgca ggtgctgggc atgtcggcgg gcgcgatccg ccgcctgggc gcccacgggg
     2101 ccatcaattc ggtgaccccg gtggtgtccg ccaccgcggg caccgtggtg gagcgcaagg
     2161 tgacccaggg ccaggtggtg cagccttcgg acagcctgtt catggtggcc gacctgtccc
     2221 aggtgtgggt caccgccgag gtgccggagc agcaggcggc cctggtgaag agcggccaga
     2281 gcgtggagat cgaggtgccc gccctgggcg cccgcctcac cggcaagctg atctacgtgg
     2341 ccgacacggt caatccggac acccgcacgg tcaccgtgcg cagcgcggtg gacaaccgcg
     2401 cgcgccagtt gaagccggcc atgctggcga ccatgctgat ccaggccgcg ccggccgagc
     2461 gtctggtggt gccggcccag gccgtggtgc gcgatgcgaa caccgacaac gtcttcgtgg
     2521 agatcgcgcc ccagcgcttc cgcctcacgc cggtgcgcct ggggcccgag gcagacggcc
     2581 gccgcgcggt gctggagggc ctcaaggcgg agcagcgcat cctcgtggct ggcgctttcc
     2641 acctcaacaa tgagcgcaag cggaaggagc tggaatgatc gaatccctcg tccgcacggc
     2701 gctgcagcag cgcctggtgg tggtcgtggt ggcggcgatc ctgctggtct tcggcctcaa
     2761 cgcggcgcgc aagctgtcgg tggatgcctt tcccgacgtg accaacgtgc aggtgcagat
     2821 cgccaccgaa gccaaggggc gttcgcccga ggaggtggag cgcttcgtga ccgtgccgct
     2881 ggagatcgcc atgaccggcc tgcccggcct ggaggagatg cgctccctca acaagcccgg
     2941 cctgtccctc atcaccctgg tgttcaccga cgccaccgac gtgtatttcg cccgccagct
     3001 ggtgatggag cgtctgctcg aggtgggcgc gcggatgccc gcgggcgtgg tgccggtgct
     3061 gggcccggtg tccaccggtc tgggcgaggt gtatcagtac accctggatc atccggagga
     3121 cggcgaccgg accctcaccg agaaggaact gacggaacgc cgcatcgtcc aggactgggt
     3181 ggtgcgcccg ctgctgcgct ccattcccgg cgtggcggag atcaactccc aggggggcta
     3241 tgcgcgccag tatcaggtgc tggtgaaccc ggaccgcctg cgtcactacc agctgacggt
     3301 gcgcgacgtg ta
//
LOCUS       DAOQPA010000216         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_21573_length_3253_cov_13.153846, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000216 DAOQPA010000000
VERSION     DAOQPA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21573_length_3253_cov_13.153846"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1126)
                     /locus_tag="PKX01_17015"
     CDS             complement(221..1126)
                     /locus_tag="PKX01_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ77888.1"
                     /translation="MPLSLLRLPSLDLVRGFVAVGRRMSITVAAADLCLTQSAVSRQI
                     HALEDHLGLKLFVRGHRSIAFTPEGERLFRSADGAFQQLQDVLGDLRAAGALRPVTVS
                     ACIGIAGLWLLPRLSRFQALHPGVDLRVSAVNRIADLRHEGIDLAIRYAPDDAVPDGA
                     ERLFGETVVPVAHAGLAGVDLADDGALAGVNLLEFDDPGRPWLQWSTWLSAQGRAAPR
                     PKGVLHFNQYDQVIQAALAGQGVALGRLALIRPLLDEGRLVCVAGPVGGGANRHAYWL
                     IQAEAEPRDEVRRVAAWIRAEASAG"
     gene            1262..2095
                     /locus_tag="PKX01_17020"
     CDS             1262..2095
                     /locus_tag="PKX01_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HMZ77889.1"
                     /translation="MTPPNRPSQHALADTFRALHADPAGFLIPNPWDAGTARLLAHLG
                     FRALASTSAGFSFGRGHADRSVPRGPLLAHLVELTAATALPVSADLENGFGDAPSTVA
                     ETIRLAAAAGVVGGSIEDATGRADAPLYDIPFAAERIRAAVEAARALPFPFTLTARAE
                     NFFIGRGDLADTIRRLQAYQEAGADVLFAPGISRTEDIATLLREVDRPVNVLLGAPGM
                     SVDAPTLFAMGVRRVSVGGSLARAALGAFLRAATELRDQGTTDYTRDAVSGKELNRLF
                     G"
     gene            complement(2144..3058)
                     /locus_tag="PKX01_17025"
     CDS             complement(2144..3058)
                     /locus_tag="PKX01_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HMZ77890.1"
                     /translation="MSDSLYRNPLARIVTSPGEILVRVAAAAAGPTGEVDAEMRALMA
                     RQVAAGELLDMNPAWIWPDFAAGLMRDAPSKMLWVLRETGALRYLLPELDALFGVPQS
                     ADDPPTVDIGEHQLRVVDAVARQHAPLAVRFAALLYNLGKADSPPEHLPSHYRHMERG
                     APRVEAVCARFGVTAEVRELALLALREVERVHRAAEMRAGSIAALLERVDAFGQPERF
                     ARLMTLCACDFHAFPGRATRPYPKARLLATALEACRGIDPAALQEDHADDPEAAAQAL
                     LEARAMAVARALRSERWDDSAQEEAAAD"
     gene            3057..>3253
                     /locus_tag="PKX01_17030"
     CDS             3057..>3253
                     /locus_tag="PKX01_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77891.1"
                     /translation="MAKAVMGGRTTKATRVWGSRRPQKHPRDSRKGPQHVGCRATGGR
                     DDDDGREGYSKMNAGWPGVTAG"
BASE COUNT          439 a         1213 c         1220 g          381 t
ORIGIN      
        1 ggtctcgacc cggggcgtgg ggagggcggc gctgcgggcc cttcacgccc ggtcggccgc
       61 cgcgccgtcg cggagcgggt cggatgcgtc catggggcct tcgcccagat gggcgggctg
      121 gactcaacgg cctttgtcac cggcgcacgg ggcaatcggg tgggtcgagc cgggcgggac
      181 agaccgcgga cgaccaccgc gtcgcgcccc gtcctgcgcc tcagccggcc gacgcctccg
      241 cgcggatcca cgcggccacg cggcgcacct cgtcacgggg ctcggcctcg gcctggatca
      301 gccagtaggc gtggcggttg gcgccgccgc cgacggggcc ggccacgcag acgaggcgac
      361 cttcgtccag caaggggcgg atcagcgcca gccggccgag ggccacgccc tggccggcaa
      421 gggccgcctg gatcacctgg tcgtactggt tgaagtgcag cacgcccttg ggccgtgggg
      481 cggcgcggcc ctgggcggag agccaggtgc tccactgcag ccaggggcgg ccggggtcgt
      541 cgaattccag caggttgacg cccgccaggg ccccgtcgtc cgccagatcc acccccgcca
      601 ggccggcgtg ggccaccggc accacggtct cgccgaacag gcgctcggcg ccgtccggca
      661 cggcgtcgtc gggggcgtag cggatggcca ggtcgatgcc ctcgtggcgc aggtcggcga
      721 tgcggttgac cgccgagacg cgcaggtcca cgccggggtg cagggcctgg aagcggctca
      781 ggcgcggcag cagccacagg ccggcgatgc cgatgcaggc gctcaccgtg accgggcgca
      841 gggccccggc ggcgcgcagg tcgcccaaca cgtcctgcag ctgctgaaag gcgccgtcgg
      901 cgctgcggaa caggcgctcg ccctcggggg tgaaggcgat ggaacgatgc ccgcgcacga
      961 agagcttgag gcccaggtgg tcctccaggg cgtggatctg gcggctcacc gccgactggg
     1021 tgaggcacag gtcggcggcg gcgacggtga tgctcatgcg ccgcccgacg gcgacgaagc
     1081 cgcggaccag gtccagggag ggcagacgga ggaggctcag gggcattccc atggcgcatg
     1141 cgaagggacg gaaaagtcga ttgaaccaca ggccgcgggc gcgtgtaatg ggcgggacgc
     1201 tgccgcgccc ggcgcgcagc ccgtcgcccg ccccgcttct tcattccgac aaggatctgc
     1261 gatgacgcct cccaaccgcc cgtcccagca cgcgctggcc gacaccttcc gcgccctcca
     1321 cgccgacccc gccggcttcc tcatccccaa cccgtgggac gcgggcaccg cgcgcctgct
     1381 ggcgcatctg ggctttcgcg cgctggccag caccagcgcc gggttctcct tcggccgcgg
     1441 ccatgcggac cgctcggtgc cccgcggccc cctgctggcg cacctggtgg agctgaccgc
     1501 ggccaccgcc ctgcccgtca gcgccgatct ggagaacggc ttcggcgacg ccccgtccac
     1561 ggtggccgag accatccgcc tggccgccgc ggcgggcgtg gtgggcggct ccatcgaaga
     1621 cgccacgggc cgcgcggacg ccccgctgta cgacatcccc ttcgccgcgg agcgcatccg
     1681 cgccgccgtg gaggcggccc gcgccctgcc cttccccttc accctcaccg cccgcgcgga
     1741 gaacttcttc atcggtcgcg gcgacctcgc cgacaccatc cgccgcctgc aggcctacca
     1801 ggaggccggc gccgacgtgc tgttcgcccc cggcatcagc cgcacggagg acatcgccac
     1861 cctgctgcgg gaggtggacc ggccggtgaa tgtgctgctc ggcgcgccgg gcatgagcgt
     1921 ggacgcaccg accctcttcg ccatgggggt gcgccgggtc agcgtgggcg gttccctggc
     1981 gcgggcggcg ctgggggcct tcctgcgcgc cgccaccgag ctgcgcgacc agggcaccac
     2041 ggactacacc cgcgacgcgg tgagcggcaa ggagctgaac cggctgttcg gctgaggcag
     2101 accggcgcgc gggcggccgc gtccggcgac gcggcgcggg cgcctaatcc gccgcggctt
     2161 cttcctgggc gctgtcgtcc cagcgttccg agcgcagggc gcgggccacg gccatcgcgc
     2221 gggcttccag cagggcctgg gcggcagcct ccggatcgtc ggcgtggtct tcctgcagcg
     2281 ccgcggggtc gatgccgcgg caggcctcca gggccgtggc gagcaggcgc gccttgggat
     2341 aaggccgcgt ggcgcggccg gggaaggcgt ggaagtcgca ggcgcacagg gtcatcaggc
     2401 gggcgaagcg ctccggctgg ccgaaggcgt ccacccgctc cagcagcgcg gcgatggagc
     2461 cggcgcgcat ctcggcggcc cggtgcaccc gctccacctc ccgcagggcc agcagggcca
     2521 gctcgcggac ctcggcggtc acgccgaagc gcgcgcacac cgcctccacc cgcggcgcgc
     2581 cccgctccat gtggcggtag tgggacggca ggtgctcggg cggcgaatcg gccttgccca
     2641 ggttgtagag cagcgccgca aagcgcaccg ccagcggcgc atgctggcgc gccacggcgt
     2701 ccaccacgcg cagctggtgc tcgccgatgt ccacggtggg cggatcgtcg gcgctctggg
     2761 gcacgccgaa gagggcgtcc agctccggca gcaggtagcg cagcgcgccg gtctcccgca
     2821 agacccacag catcttcgac ggcgcgtcgc gcatcagccc cgccgcaaaa tccggccaga
     2881 tccacgccgg attcatgtcc agcagctccc ccgccgccac ctgccgggcc atcagggcgc
     2941 gcatctccgc gtccacctcc cccgtcggcc ccgccgccgc cgccgccacg cgcacgagga
     3001 tctcccccgg cgaggtgacg atgcgggcga ggggattgcg gtagagggaa tcggacatgg
     3061 cgaaggctgt gatgggtgga aggacgacaa aagcgacccg cgtgtggggt tcgcgacgcc
     3121 cgcaaaaaca cccccgcgac agccgcaaag gcccgcagca cgtgggatgt cgggccacgg
     3181 gcgggcgcga cgacgacgac ggtcgggagg gttacagcaa gatgaatgcc gggtggccgg
     3241 gcgtcaccgc ggg
//
LOCUS       DAOQPA010000217         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_21986_length_3218_cov_20.162188, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000217 DAOQPA010000000
VERSION     DAOQPA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21986_length_3218_cov_20.162188"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKX01_17035"
     CDS             <1..260
                     /locus_tag="PKX01_17035"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943328.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMZ77892.1"
                     /translation="PFRDAHEAVALAVRAADARGCDLPQIPLADLRAAMAHVPGAVER
                     MGDDVFQVLTVEGSLAARNHVGGTAPEQVRAAIARARARLG"
     gene            272..1552
                     /locus_tag="PKX01_17040"
     CDS             272..1552
                     /locus_tag="PKX01_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ77893.1"
                     /translation="MERIGKYEIRRLLGEGSTSAVYLGFDPFTQRDVAIKRLHPEVTR
                     NSSRATLYRQLLLNEAVLAGQLHHPHIAQIHDAVIDEDDAYVVMEYVPGGTLEPFTDP
                     ARLLPFERLIEIIFKCTRALDYAHLQGITHRDVKPANILLTSPDGQDIKVSDFGAALF
                     TASETTQLAGLGSPAYMSPQQVRELPVDHRTDIYSLGVVMFQLLTGRLPFEATSNVNL
                     IYQIAHHVPPPPSSVRPEVPPELDAIVGRATQRDLADRYATWAEFSHDLAEAYRNRDL
                     AVSPREPRDSEKFERLRAFRFFREFSDVEIWEIVRFAEWHLYPPGTVVMKEGEPGNHF
                     CFVVQGTLRVVKGGHLLNVLTPGDCFGEMALFSTGRGARTASVEAEDATRVLTIRAPS
                     LLRASSECQMHFYKGFLEVLAARLTLANRRITGP"
     gene            complement(1628..1939)
                     /gene="grxD"
                     /locus_tag="PKX01_17045"
     CDS             complement(1628..1939)
                     /gene="grxD"
                     /locus_tag="PKX01_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HMZ77894.1"
                     /translation="MDVQQIIHEQVTTHPVVLYMKGTPAMPRCGFSSLAAQILNACGV
                     KEFFSVDVLADEAIRQGIKEYANWPTIPQLYIKGEFVGGSDIMREMYESGELKQMLAA
                     D"
     gene            complement(1984..2844)
                     /gene="prmC"
                     /locus_tag="PKX01_17050"
     CDS             complement(1984..2844)
                     /gene="prmC"
                     /locus_tag="PKX01_17050"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMZ77895.1"
                     /translation="MDAPATLGAALVAARGRIPQSEARLFLRAIAGVSAAQLAAYPER
                     DLTPAQAARFADWLARRTAGEPVAYLLGEREFYGRCFRVTPATLIPRPETELIVDWAK
                     TRLAPDAPARVLDLGTGSGALAVTLALEWPAAAVTGIDRSPAALAVAAANGAALGASV
                     RWLESDWCAALEPAERFDLIVSNPPYVAADDPHLAEGDVRFEPRSALASGPRGLDDIA
                     RIAVTAPPFLVPGGWLIVEHGYDQAEAVRALLAEQGLGAVDSARDLAGIERISFGRCP
                     DGLDALRRGA"
     gene            complement(2844..>3218)
                     /locus_tag="PKX01_17055"
     CDS             complement(2844..>3218)
                     /locus_tag="PKX01_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HMZ77896.1"
                     /translation="INKTDSAVRITHLPTGIVAECQDGRSQHQNKASALRVLAARIKD
                     VQVREQQAREAATRKSLVGSGDRSERIRTYNFPQGRLTDHRINLTLYKLDAVMQGEMD
                     DVIAALTAEHQAELLAALADEA"
BASE COUNT          488 a         1172 c         1061 g          497 t
ORIGIN      
        1 tgcccttccg cgacgcccac gaggccgtgg ccctcgccgt acgcgccgcc gacgcgcgcg
       61 gctgcgacct gccccagatt ccgctggccg acctgcgcgc cgccatggcc cacgtgccgg
      121 gcgccgtcga gcgcatgggc gacgacgtgt tccaggtcct caccgtggaa ggttccctcg
      181 ccgcccgcaa tcatgtgggc ggcacggcgc cggagcaggt gcgcgccgcc atcgctcgcg
      241 cccgcgcgcg tctgggctga ggcgcgcggc catggagcgg atcggcaagt acgagatccg
      301 gcgcctgctg ggagaaggct ccaccagcgc cgtctatctt ggcttcgatc ccttcaccca
      361 gcgggacgtg gccatcaagc gcctgcaccc cgaggtgacc cgcaattcgt cgcgcgccac
      421 cctgtaccgc cagctcctgc tcaacgaggc cgtgctggcg ggccagctcc atcatcccca
      481 catcgcccag atccacgacg cggtcatcga cgaggacgac gcctacgtgg tgatggagta
      541 cgtgcccggc ggcaccctgg agcccttcac cgatcctgcc cggctgctgc ccttcgagcg
      601 cctgatcgag atcatcttca agtgcacgcg ggccctcgac tacgcccacc tgcagggcat
      661 cacccaccgg gacgtgaagc cggccaacat cctgctcacc tcgccggacg gccaggacat
      721 caaggtctcg gacttcggcg cggccctgtt caccgcctcc gaaaccaccc agctcgccgg
      781 cctcggttcg ccggcgtaca tgtcacccca gcaggtacgc gagctgccgg tggatcaccg
      841 caccgacatc tattccctcg gcgtggtgat gttccagctc ctcaccggcc gtctgccctt
      901 cgaggccacg tccaacgtca atctgatcta ccagatcgcc caccacgtcc cgcctccgcc
      961 gtcgagcgtc cgcccggagg tgccgcccga gctggacgcc atcgtcggac gcgccaccca
     1021 gcgggacctg gccgaccgct acgccacctg ggccgagttc tcccacgatc tggccgaggc
     1081 ctaccgcaac cgcgatctcg ccgtgagccc gcgggagccg cgggacagtg aaaagttcga
     1141 acgcctccgc gccttccgtt tcttccgcga attcagcgac gtggagatct gggagatcgt
     1201 gcgcttcgcc gagtggcacc tgtacccgcc gggcaccgtc gtcatgaagg aaggcgaacc
     1261 gggcaaccat ttctgcttcg tcgtgcaggg caccctgcgc gtcgtgaagg gcggacacct
     1321 cctcaacgtg ctcacgccgg gcgactgctt tggcgagatg gccctcttct ccaccgggcg
     1381 cggggcccgt acggcctcgg tcgaagccga agacgccacc cgcgtcctga cgatccgtgc
     1441 cccgtccctg ctgcgcgcct ccagcgagtg ccagatgcac ttctacaagg gtttcctcga
     1501 agtcctcgcg gcgcgcctga ccctcgccaa ccggcgcatc accgggccct gagcggcgct
     1561 cgccgatcac agcccgcaaa aaagccgccc gcgggcggct tgagcgaacg ggatgctgtt
     1621 cgggcgctca atcggcagcc agcatctgct tgagttcgcc ggactcgtac atctcgcgca
     1681 tgatgtcgga gccgccgacg aattcgccct tgatgtagag ctgcggaatg gtcggccagt
     1741 tggcgtattc cttgatgccc tggcggatgg cctcgtcggc cagcacgtcc accgagaaga
     1801 attccttcac gccgcaggcg ttcaggatct gcgcggccag cgacgagaaa ccgcagcgtg
     1861 gcatggccgg cgtacccttc atgtacagca cgaccgggtg ggtggtgact tgttcgtgga
     1921 tgatctgttg gacgtccatc gtggtgttct cgctaaatag ttgagcaatt cagtcggaaa
     1981 agtctaggcc cctcgccgca gggcgtcaag cccgtccgga caccgcccga agctgatccg
     2041 ctcgatgccg gccagatctc gcgccgaatc cacggcaccc agaccctgct ccgcaagcaa
     2101 ggcgcgtacc gcttccgcct ggtcatagcc gtgctcgacg atcagccagc cgcccggcac
     2161 cagaaatggc ggcgcggtca cggcgatgcg tgcaatgtcg tccagcccgc gggggcccga
     2221 agccagtgcg gagcgcggtt caaagcgcac atcgccctcg gccagatggg gatcgtcggc
     2281 cgccacgtag ggcggattgc tgacaatcag gtcgaaacgc tctgccggtt ccagcgccgc
     2341 gcaccagtcg ctctcgagcc agcgcaccga ggcgccgagg gccgccccgt tggccgccgc
     2401 caccgccagc gcggcgggcg aacggtcgat gcccgtcacc gcggcggccg gccactccag
     2461 tgccagggtc accgccagag caccgctgcc ggtgcccagg tcgaggacac gggccggcgc
     2521 atccggcgcc agccgggtct tggcccagtc cacaatcagc tcggtctccg ggcggggaat
     2581 cagcgtggcg ggcgtgacgc ggaagcaccg tccgtagaat tcccgctcgc ccagcaggta
     2641 ggccaccggc tcgccggccg tgcggcgggc cagccagtcg gcgaagcggg cggcctgcgc
     2701 cggcgtcagg tcccgctccg gataggcggc gagctgggcc gccgacacgc cggcaatggc
     2761 ccgcaggaag aggcgtgcct cgctctgggg aatccgcccg cgggccgcga caagggccgc
     2821 gccgagggtc gccggcgcgt ccatcaggcc tcgtccgcaa gggcggccag gagctcggcc
     2881 tggtgctcgg cggtgagggc ggcgatgacg tcatccatct cgccctgcat caccgcgtcg
     2941 agcttgtaga gggtcaggtt gatgcggtga tcggtgaggc ggccctgggg gaagttgtag
     3001 gtgcggatgc gctcggagcg gtcgccgctg cccaccaggc tcttgcgcgt ggcggcctcg
     3061 cgggcctgct gctcacgcac ctgcacgtcc ttgatgcgcg ccgccagcac gcgcagggcc
     3121 gaagccttgt tctggtgctg ggagcggccg tcctggcatt cggcgacgat gccggtgggc
     3181 aggtgggtga tgcgcaccgc cgagtcggtc ttgttgat
//
LOCUS       DAOQPA010000218         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_21987_length_3218_cov_18.790073, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000218 DAOQPA010000000
VERSION     DAOQPA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21987_length_3218_cov_18.790073"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="gap"
                     /locus_tag="PKX01_17060"
     CDS             complement(<1..910)
                     /gene="gap"
                     /locus_tag="PKX01_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ77897.1"
                     /translation="MSIKVAINGFGRIGRCTLRAIYEQGLQNEFDVVAINASGDLATN
                     AHLLKYDTTHGRFATSVETEGDNVIIIDGKKIPFYSTKDPKGVNWGSHGVDVLLECTG
                     AYTTKAKAQALLEQGAQRVLISAPGGDDVDTTIVMGVNEGDLKAGYTVVSNASCTTNC
                     LAPVAKILADSIGIKQGLMTTIHAYTNDQVTVDVRHKDLRRARAAAANIIPTKTGAAK
                     AVGLVLPQLKGKVDGFALRVPTINVSLVDLTFTADRATTKEEINELMTAAANGPLKGI
                     LAVNTEPLVSSDFNHTTVSSTFDATQT"
     gene            complement(971..3001)
                     /gene="tkt"
                     /locus_tag="PKX01_17065"
     CDS             complement(971..3001)
                     /gene="tkt"
                     /locus_tag="PKX01_17065"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMZ77898.1"
                     /translation="MSSTRNVKPPVFNPLTGAIRALAMDAVQKANSGHPGAPMGMAEI
                     AEVLWRRHLRHNPANPRWADRDRFVLSNGHGSMLIYALLHLTGYDLSIDDLKAFRQLH
                     SKTPGHPEVGYTPGVETTTGPLGQGITNAVGMALAEKILADTFNRPGHRVVDHHTYVF
                     LGDGCLMEGISHEACSLAGTWGLGKLIAFYDDNDISIDGHVGGWFTDDTPKRFEAYGW
                     HVVRDVQGHDPVEIEAALKAAQAETGRPSLICCKTVIGAGAPNKQGGHDVHGAPLGAA
                     EIEAARAYIGWTHPPFEIPADVYAAWDARTAGAAAEADWNTRFAVYAAEFPELAAEFT
                     RRMAGDLPADWAAHVDAVLAQVTAKGETIATRKASQNSIEAYAPKLPELLGGSADLAG
                     SNLTLWSGARGVTRSTGGNYVYYGVREFGMAAIANGVSLHGGLIPYTATFLMFSEYAR
                     NALRMAALMKARQIFVFTHDSIGLGEDGPTHQPVEQIATLRLIPNMDVWRPCDTTETA
                     VAWAAAVERRDGPTALAFSRQNLPFVPRSAGQIADIRRGGYVLSEAGTPSQPIAPQAI
                     IISTGSEVALALAAQKSLAADGIAVRVVSMPSTTAFDRQDAAYRCAVLPKGLPRVAVE
                     AGVTDIWRKYVGLEGEVIGLDRFGESAPAGELFKQFGITAEAVVGAVKQVIA"
BASE COUNT          475 a          932 c         1224 g          587 t
ORIGIN      
        1 gggtctgggt ggcgtcgaag gtggaggaga cggtggtgtg gttgaagtcg gaggacacca
       61 gcggctcggt gttcaccgcc aggatgccct tgagggggcc gttggcggcg gcggtcatca
      121 gctcgttgat ctcttccttg gtggtcgcgc gatcggcggt gaaggtcagg tccaccaggg
      181 agacgttgat ggtcggcacg cgcagggcga agccgtccac cttgcccttg agctggggca
      241 gcaccagccc gacggccttg gcggcgccgg ttttggtggg gatgatgttg gcggccgcgg
      301 cgcgggcacg gcgcagatcc ttgtggcgga cgtccacggt cacctgatcg ttggtgtagg
      361 cgtggatggt ggtcatcaga ccctgcttga tgccgatgct gtccgccagg atcttggcta
      421 caggggccag acagttggtg gtgcaggagg cgttggagac gacggtgtag ccggccttca
      481 ggtcgccttc gttcacgccc atgacgatgg tggtgtccac gtcgtcgccg ccgggggcgg
      541 agatcagcac gcgctgggcg ccttgctcca gcagggcctg ggccttggcc ttggtggtgt
      601 aggcgccggt gcattcgagc agcacgtcca cgccgtggct gccccagttc acgcccttgg
      661 ggtccttggt ggagtagaag gggatcttct tgccgtcgat gatgatgacg ttgtcgcctt
      721 cggtctccac ggaggtggca aagcggccgt gggtggtgtc gtacttcagc aggtgcgcgt
      781 tggtggccag atcgccggag gcgttgatcg ccaccacgtc gaattcattt tgcaggccct
      841 gttcgtagat ggcgcgcagc gtgcagcggc cgatgcgacc gaaaccgttg atggcgacct
      901 tgatgctcat cgagttttct cctggttgga atgctggtaa cgataatccc tgcccgccgc
      961 gcgctgcgcg tcaggcgatg acttgcttta ctgcgccgac caccgcttcg gcggtaatgc
     1021 caaactgctt gaacagctcg ccggccgggg ccgattcgcc gaagcggtcg agcccgatga
     1081 cttcgccctc aaggcccacg tacttgcgcc agatgtcggt gacgccagcc tcgacggcca
     1141 cgcggggcag gcctttgggc agcacggcgc agcggtaggc ggcgtcctgg cggtcgaagg
     1201 cggtggtgct gggcatggag accacacgca ccgcaatgcc gtctgcggcc agggacttct
     1261 gggcggcgag ggcgagggcg acctcggaac cggtcgatat gataatggcc tgcggcgcaa
     1321 tcggctgtga cggagttcct gcctccgaca gaacatagcc gccgcgacgg atgtcggcga
     1381 tctgtccggc cgagcgcggc acgaagggca ggttctggcg cgagaaggcc agggccgtgg
     1441 ggccatcgcg gcgttcgacg gcggcggccc aggccacagc ggtctcagtg gtgtcgcagg
     1501 ggcgccagac gtccatgttg gggatcaggc gcagggtggc gatctgctca accggctggt
     1561 gggtggggcc atcctcgccg aggccgatgg agtcgtgggt gaatacgaag atctgccgcg
     1621 ccttcatcag cgccgccatg cgcagggcat tgcgggcgta ttcgctgaac atcaggaagg
     1681 tggcggtgta ggggatcagg ccgccgtgca gggacacgcc gttggcgatg gcagccatgc
     1741 cgaattcgcg cacgccgtag tacacatagt tgccgcccgt gctacgggtg acgcccctgg
     1801 cgccggacca aagggtgagg ttggagccgg ccaggtcggc ggagccgccg aggagttccg
     1861 gcaacttggg tgcgtaggct tcgatgctgt tctggctggc cttgcgggtg gcgatggtct
     1921 cgcccttggc ggtgacctgg gcgaggaccg cgtccacgtg ggccgcccag tcggcgggca
     1981 ggtcgccggc catgcggcgg gtgaattcgg cggcgagctc ggggaattcg gcggcgtaga
     2041 cggcgaagcg ggtgttccag tcggcttcgg cggcggcgcc tgcggtacgg gcatcccagg
     2101 ccgcataaac atcggccggg atctcgaagg gcggatgggt ccagccgatg taggcgcggg
     2161 ccgcctcgat ctcggcggcg ccgagcggcg cgccgtggac gtcgtgcccg ccctgcttgt
     2221 tgggggcgcc ggcgccgatc acggtcttgc agcagatcag gctggggcgg ccggtttcgg
     2281 cctgggcggc cttcagggcg gcttcaatct cgaccgggtc gtggccctgg acgtcgcgca
     2341 ccacgtgcca gccgtaggct tcgaagcgct tcggcgtgtc gtcggtgaac cagccgccca
     2401 cgtggccgtc gatggagatg tcgttgtcgt cgtagaaggc gatcagcttg cccagacccc
     2461 aggtgccggc gagggaacaa gcttcgtggg agatgccttc catcaggcag ccgtcaccga
     2521 ggaacacgta ggtgtggtgg tcgacgacac ggtggccggg ccggttgaag gtgtcggcga
     2581 ggatcttttc ggccagcgcc atgcccacgg cgttggtgat gccctggccg agggggccgg
     2641 tggtggtctc gacgcccggg gtgtagccga cttccgggtg gcccggggtc ttgctgtgga
     2701 gctggcggaa ggccttgagg tcgtcgatgg acagatcgta gccggtgagg tgcagcaggg
     2761 catagatcag catcgaaccg tggccgttgg acagcacgaa gcggtcacga tcggcccagc
     2821 ggggattggc ggggttgtgg cgcagatgtc ggcgccacag gacttcggcg atctccgcca
     2881 tgcccatcgg ggcgccgggg tgcccggaat tggccttctg gacggcgtcc atggccagcg
     2941 cacgaatcgc gccggtcaga gggttgaaga caggcggctt gacgttacgc gtagaagaca
     3001 tcgactggac cttcggccgg cggggcggcg cgcaaagacc gcaattatcg tggattttcc
     3061 tgtacttgga tagaggctgc aggccgtcgc ccgggcgtgc ggcggcgcac gatggcgaaa
     3121 tggaaaatac ggcataatcc gggcgcccct tacatttcct tacttttgtg atcttcgcgc
     3181 gatggcagct tttccgcgtg gccggcgacc gctcctgg
//
LOCUS       DAOQPA010000219         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_22607_length_3165_cov_14.546302, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000219 DAOQPA010000000
VERSION     DAOQPA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22607_length_3165_cov_14.546302"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /locus_tag="PKX01_17070"
     CDS             <1..2117
                     /locus_tag="PKX01_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HMZ77899.1"
                     /translation="TLLLGAAALLVAAGSFHLWRRAERAGEIALSRQLAAQSATALAE
                     AAPDTALRLAAAAGQHASSFEAQDSLLAALAQAPLDWRSAGQQGLFSLAVRADGGLIA
                     SGGRDGSIRLWRGGDDLTPAARLDAHKEQVLALAFSPDGQRLASAGIDRRVILWDVRD
                     PARPQRLAERADHEGPVLAVAFSPDGQRLASAGSDRRIVLRDGHSGQPVGPVLDAHGD
                     TVTGLAFSPDGRLLASTGYDGALLLWATDGTATPRLSASHPAVLYGVAFSPDGNLIAT
                     AGADRHVVLWQSTTLAVRDAPLTDHDAAVRAVAFSPDGQRLISVGDDRQVLEHDLTRP
                     TSLGRNHPGHRDRALAVAFTAAGDAFSVGADDRLIHHGQTLGSTLATRLPGVDLPAWA
                     LAWSPDGRQLASAGPDGAILLRDALSGAPVGQARRGHEALVTGLAFTPDGTLLASAGQ
                     DGRLFLWPVAGGPPRALQPAGEALQTVTVSPDGRTLAAAGRDGHILRWTLPEATPLPR
                     LEHGAAVSDLAFAPDGRRLASAGRDGTLRLWNADGTAADRLDLPGDSLTRIAWPAGDG
                     PLALATRTRGLLQWTPGDAAPRPLGNDEHALLALSFSPDGRQLASADVNGRVRLWDMV
                     SGQAFHAPVQRFPGPVLALAFAPDGRRLAGTGWGGGVQLTPIGADLWSRMACERLRPL
                     PPAPPESACPAPENGRATTSRP"
     gene            2228..>3165
                     /locus_tag="PKX01_17075"
     CDS             2228..>3165
                     /locus_tag="PKX01_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77900.1"
                     /translation="MALIIPGVEVKVVKEVLSPQLAPSGVLGLVGIVEAPGSGIGRAA
                     SWRSFTDRFGAASAFSLPEAAQALGNGVFELVVCPVACDATASLKVPAATGNAAAFTL
                     SARAPGPWGNALQVSLATRQVATGKKLDSGDDETRQVFDLTIRIAGSDYKEVHRNLST
                     DATDTLRHVRDVLAAQSQLLRVVEERDKLKPPKDSPDVKDPSAALLGLTGGADADPDA
                     YRKAIALLENESDVDMVLVSVQDTRDTTRVGRIYADVISHCDRMSARSQGRIGFGEVP
                     GGNAADIATAADKLTGTLVSDRFILVAPAGVVGAVAG"
BASE COUNT          423 a         1337 c         1003 g          402 t
ORIGIN      
        1 ccaccctgct gctgggtgcc gccgcgctcc tcgtcgccgc cggcagcttc catctgtggc
       61 ggcgcgccga gcgggccggc gagatcgccc tgtcgcgcca gctcgccgcc cagtccgcca
      121 ctgccctggc cgaagccgcc cccgacacgg ccctgcgtct ggccgccgcg gccggtcagc
      181 acgcctccag cttcgaagcc caggacagcc tcctcgccgc cctcgcccag gcgcccctcg
      241 actggcgcag cgccggacag cagggactct tcagtctcgc cgtgcgcgcc gatggcggcc
      301 tcatcgccag cggcgggcgg gacggcagca tccgcctgtg gaggggcggc gacgatctga
      361 cgcccgccgc ccgcctcgac gcccacaagg agcaggtcct cgccctggcc ttctcgccgg
      421 acggccaacg ccttgccagc gctggcatcg accgccgggt gatcctgtgg gatgtgcgcg
      481 acccggcccg gccccaacgc ctcgccgaac gtgccgacca cgaaggcccg gtccttgccg
      541 tggccttttc gccggacggc cagcgcctcg ccagcgccgg ctccgaccgc cgcatcgtcc
      601 tgcgcgacgg ccacagcggt cagcccgtcg gtcccgtcct cgacgcccac ggcgacaccg
      661 tcaccggcct cgccttcagc ccggacggcc gcctgctcgc ctccaccggt tacgacggcg
      721 ccctgctcct gtgggccacc gacggcaccg ccacgccacg cctgagcgcc agccaccccg
      781 ccgtgctgta tggcgtcgcc ttctcacccg atggcaatct gatcgccacg gccggtgccg
      841 accgccacgt cgtcctgtgg cagagcacca ccctcgccgt gcgcgacgca ccgctcaccg
      901 accacgacgc cgcggtgcgg gccgtcgcct tcagcccgga cggacagcgt ctgatctccg
      961 tcggcgacga ccgccaggtg ctggagcacg acctcacgcg ccccacaagc ctcggccgca
     1021 accatcccgg ccaccgggac cgcgccctgg cggtcgcctt cacggccgcc ggcgatgcct
     1081 tcagtgtcgg tgccgacgac cgcctcatcc accacggcca gaccctcggt tcgaccctcg
     1141 ccacacgcct gccgggcgtc gacctgcccg cctgggccct cgcctggagc ccggacgggc
     1201 ggcagctcgc cagcgccggc ccggacggcg ccatcctact gcgggacgcc ctcagcggtg
     1261 cgcccgtcgg ccaagcgcga cgcggccacg aagccctcgt caccggcctc gccttcaccc
     1321 cggacggcac cctgctcgcc agcgccggcc aggacggccg cctgttcctg tggcctgtcg
     1381 ctggcggacc gccgcgcgcc ctgcagccgg ccggcgaggc gctgcagacc gtcaccgtca
     1441 gcccggacgg ccggaccctc gccgccgccg gccgggacgg acacatcctg cgctggacgc
     1501 tgcccgaggc caccccgctg ccccgcctgg agcatggcgc tgcggtcagc gacctcgcct
     1561 tcgccccgga tggccgccgg ctcgccagcg cggggcgcga cggcaccctg cgcctgtgga
     1621 atgccgacgg caccgcagcc gaccgcctgg atctgcccgg cgacagcctg acgcgcatcg
     1681 cctggcccgc cggagacggc cccctggccc tggccacgcg cacccgcgga ttgctgcaat
     1741 ggacgcccgg cgacgcggcc ccgcgccccc tcggcaacga cgaacacgcc ctgctcgccc
     1801 tcagcttttc gccggacggc cgccagcttg ccagcgcaga cgtgaacggg cgggtgcgcc
     1861 tgtgggacat ggtcagcggg caggccttcc acgcccccgt ccagcgcttc cctggcccgg
     1921 tgctggccct cgccttcgcg cccgacggtc gccgcctggc gggcaccggc tggggcggcg
     1981 gggtgcagct cacgcccatc ggggccgacc tctggagccg catggcctgc gaacgcctgc
     2041 gccctctccc gccagcccct ccggaatccg cctgccccgc ccccgagaac ggccgggcga
     2101 cgacatctcg accttgacat cggcaagtcc agatctaatc tgattagacg gccggcccag
     2161 cagcgccggg ccgtggcatt ctcatcccgc gccacatatc tgccggcgcc ctttcggaga
     2221 gcttcccatg gcattgatca tcccaggcgt cgaagtcaag gtcgtcaagg aagtcctctc
     2281 gccccaactg gccccctccg gcgtgctggg gctggtcggc atcgtcgagg cgccgggcag
     2341 cggaatcggt cgcgccgcca gctggcgcag cttcaccgac cgcttcggcg ccgccagtgc
     2401 cttcagcctg cccgaagccg cccaggcgct tggcaacggt gtcttcgaac tggtcgtatg
     2461 cccggtcgcc tgcgacgcca ccgccagcct caaggtgcct gccgccaccg gcaacgccgc
     2521 cgccttcacc ctcagcgccc gcgcgccggg cccgtggggc aatgcgctcc aggtcagcct
     2581 cgccacccgc caggtggcca ccggcaagaa gctcgacagt ggcgacgacg agacccggca
     2641 ggtcttcgat ctgaccatcc gcatcgccgg cagcgactac aaggaagtac accgcaacct
     2701 gtccaccgat gccaccgaca ccctccgcca cgtgcgcgac gtactcgccg cccagtccca
     2761 gctcctgcgc gtcgtcgaag aacgcgacaa actcaagccc cccaaggaca gcccggacgt
     2821 caaggatccc agtgccgcgc tgctgggtct gaccggcggc gccgacgccg accccgacgc
     2881 ctaccgcaag gccatcgcgc tgctggaaaa cgaatccgac gtggacatgg tgctggtctc
     2941 ggtgcaggac acccgcgaca ccacccgggt cggccgcatt tacgccgatg tgatcagcca
     3001 ctgcgaccgc atgagcgccc gcagccaggg ccgcatcggc ttcggtgaag tgcccggcgg
     3061 caacgcggcg gacatcgcca ccgcggccga caagctcacc ggcaccctcg tctccgaccg
     3121 cttcatcctc gtcgccccgg ccggcgtggt gggcgcggtg gccgg
//
LOCUS       DAOQPA010000220         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_22632_length_3162_cov_15.768265, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000220 DAOQPA010000000
VERSION     DAOQPA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22632_length_3162_cov_15.768265"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKX01_17080"
     CDS             <1..1124
                     /locus_tag="PKX01_17080"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HMZ77901.1"
                     /translation="EIAQALYETHKATTYPRSDSGYLPESMFAEVPTVLDSLLKTDPT
                     LAQIMGQLDRTQRSRAWNDGKVTAHHGIIPTLEPANLSAMSEKERALYRLIRAHYLAQ
                     FLPHHEFDRTVADLSCGQQKLVATGKQVVARGWRLVLAESEREGSADEDGDAPVRTQV
                     LPALRDGMACQVAGADIKALKTMPPKPYTQGELVKAMKGVARFVTDPRLKQKLKDTTG
                     IGTEATRANIISGLIARGYIVKKGRSIRASDAAFTLIDAVPAAIADPGTTAVWEQALD
                     MIEGGQLTLDVFIGKQAAWISQLIAQYGSTSLSIKVPQGPACPQCGAPTRQRTGKSGP
                     FWSCSRYPDCKGTLPVATGTSRRGASRPRRTSGGGRKGA"
     gene            2054..2611
                     /locus_tag="PKX01_17085"
     CDS             2054..2611
                     /locus_tag="PKX01_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77902.1"
                     /translation="MDTITRQDRITLKNLKVADFASEETLCFTATVMFDGRPIAEARN
                     DGHGGSTFVRALQGQAALLAQAEEFAKSLPPASLDVEREDDEPLLIDMTLDFLVDQLA
                     DAMHAERKLRTAFNRDIGNKVLFIKDGRLLFLKGIKLKAIADRAAYFAKLRSRQDQPI
                     VILAELPADEAFAIWKQHVLGDKPR"
     gene            2945..>3162
                     /locus_tag="PKX01_17090"
     CDS             2945..>3162
                     /locus_tag="PKX01_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77903.1"
                     /translation="MSQQASFGRFGQLALTYCGKFLPLEVLHSAAGHYIGTRDTEGPV
                     SRESREYFRSHAAAQRALERGGWSQLAVP"
BASE COUNT          548 a         1146 c          960 g          508 t
ORIGIN      
        1 tggagatcgc ccaggccctg tacgagacgc acaaggccac gacgtacccg cgctcggatt
       61 cgggctacct gcccgaaagc atgttcgccg aagtgcccac ggtcctggac agcctgctca
      121 agaccgatcc cacgctggcc cagatcatgg gccagctcga ccgcacccag cgctcgcgcg
      181 cctggaacga cggcaaggtg acggcgcacc acggcatcat cccgacgctc gaacccgcga
      241 atctctccgc catgagcgag aaggaacggg cgttgtaccg gctgatccgg gcacattacc
      301 tggcccagtt cctcccgcac cacgagttcg accgcaccgt ggcggatctc tcctgcggcc
      361 agcagaagct ggtggctacg ggcaagcagg tcgtcgcccg cggctggcgc ctggtgctgg
      421 ccgagtccga acgtgaaggc agcgccgatg aggatggcga cgcccccgtg cgcacccagg
      481 tgctgcctgc gctgcgtgac gggatggcat gccaggtcgc cggggccgac atcaaggccc
      541 tcaagacgat gccgcccaag ccctataccc agggcgaact ggtcaaggcg atgaagggcg
      601 ttgcgcgttt cgtgaccgac ccgcgcctga agcagaagct caaggacacg acgggcatcg
      661 gcaccgaggc gacgcgggcc aacatcatca gcgggctgat cgcccgcggc tatatcgtga
      721 agaaggggcg ctccatccgc gcatcggatg cggcgttcac gctgatcgac gccgtgcccg
      781 cggcgattgc cgaccccggc accaccgccg tctgggaaca ggcgctggac atgatcgagg
      841 gcggacaact caccctggat gtgttcatcg gcaagcaggc cgcctggatt tcgcagttga
      901 tcgcgcaata cggcagcacg tccctgtcca tcaaggttcc ccaaggaccg gcttgtccgc
      961 agtgcggcgc acccacgcgc cagcgaaccg gcaagagcgg cccgttctgg tcgtgcagtc
     1021 gctaccccga ctgcaaaggc acgctgccgg tggcaaccgg cacgtccagg cgtggtgcct
     1081 cgcgtccacg ccgtaccagt ggcggtggcc gcaaaggcgc ctgaccgccc ccgttccccg
     1141 tgagccatgc ccgccatcgg cggcgtggcc cgtgtcccgc atgccctgcg ggacgcccca
     1201 gcgcgcaacg ccttcttgct ccgtgtgcgc gtcccgtccg gccggccccg gccgcgggac
     1261 ctgaaggtgg cttctccgcg aaccgcaccc cgcgcgttct cctcgtctgc attccttccg
     1321 ccgctgcgaa gggtcccccg atggcttgcc tgtgcgagcc atccggagac ccttcgcggt
     1381 cagcggtatt cggtgccggt gcccgccggc gcaaaaacgg gctccctttg tgcgcggatg
     1441 tgcgccagac gatgccggcc ccagccacga catgggccgg gtgtgattgc tggatgagca
     1501 gacggttcta gcgacgaccg ggcctgccaa tcagcccacg ggtggttctt ctttcctccc
     1561 gagccgaagg cccctggggc cttcggcgcc tttctcctgc ctttcaacgc cccggccagg
     1621 gcgatggccc tgcgccacac gaacgggaga cccacatgac cgccaacatt gcaatccgcc
     1681 tcactacgca ccgccgatgc accgaactgc aaggcgatcg gcaattccat ggccgtgctg
     1741 tgcgtcgcgt ggctggggac gccggctggt gcagtacctg cacaagacgg gatcgatcgc
     1801 ttcggattga tgcaagacgc ggccggcggg tcgcgcgatc ctccctcctg cattgccgac
     1861 gcatcggcaa cagccagcag ccagggtgtc cagactgccg tgggttccgc ccacgccacc
     1921 cgccagttcg ccccggcatc tctccggcga acgctgccca ccgggcatcg atgccacctt
     1981 cctccaaccc acgggatgta tgccgcgtcc cgtcaggggc gtgtcgtcaa cccggtcgat
     2041 atcaggactt cccatggaca ccatcacccg acaagaccgc atcaccctca agaacctcaa
     2101 ggtcgcggat ttcgcgagcg aggaaacgct gtgcttcacc gccaccgtca tgttcgacgg
     2161 caggcccatc gccgaagccc gcaacgacgg acacggcggc tcgacgttcg tgcgcgcgtt
     2221 gcaaggccag gccgcgctgc tggcccaggc cgaggaattc gccaagagcc tgccgccggc
     2281 atcgctcgac gtcgaacgcg aagacgacga gccgcttctc atcgacatga cgctcgactt
     2341 cctagtggac caattggccg atgccatgca cgcggagcgc aagctgcgca ccgcgttcaa
     2401 ccgcgacatc ggcaacaagg tgctgttcat caaggacggc aggctgctgt tcctcaaggg
     2461 catcaagctc aaggccattg ccgaccgcgc agcgtatttc gcaaagctgc gcagccgaca
     2521 ggaccagccc atcgtcatcc tggccgagct gccagcggac gaggcattcg ccatctggaa
     2581 gcagcatgtc ctgggcgaca agccccgctg atccagcctc accgcaccgt catgaccgcc
     2641 ccgcacttgc tgcggggctt ttctttgtct ctgcgtacat caatcggggc tggaccagat
     2701 gccgatttcc aactgacgtg gcgcgccccc cccacgaagc tgcccgcatg ctcgctgatt
     2761 cgtcagcaca tgccagcagc cagggtttca ggctgccgcg gggttctccc cgcgcaaccc
     2821 accagtccgc atcgcatcca ttgcgcggac gcacgtcgcc aagacgctga tgcttttttt
     2881 caaccgcgtg cgggagctgc catcccgtga gggacagggc cccgcttttc caaggagccc
     2941 atccatgtcc cagcaagcct ctttcggccg attcggtcaa ttggccttga cctactgcgg
     3001 caagttcctg ccgctcgaag tcctgcacag cgccgccggc cactacatcg gcacgcgcga
     3061 taccgaaggt cccgtttcgc gggaatctcg tgagtacttc cgcagccatg ccgcggctca
     3121 acgtgccctc gaaagaggcg gctggtccca gctcgccgtt cc
//
LOCUS       DAOQPA010000221         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_23032_length_3131_cov_18.286736, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000221 DAOQPA010000000
VERSION     DAOQPA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23032_length_3131_cov_18.286736"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="ruvB"
                     /locus_tag="PKX01_17095"
     CDS             complement(<1..1040)
                     /gene="ruvB"
                     /locus_tag="PKX01_17095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMZ77904.1"
                     /translation="MIETDKLKGGAPDPAARLIGARSNGAQEDALERALRPKRLADYV
                     GQQKIRDQLEIFIQAARRRSEALDHVLLFGPPGLGKTTLAHIVAAEMGVNLRQTSGPV
                     LERAGDLAAILTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSV
                     KLDLPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYTAEELAWIVSRSAGLLDVQIED
                     TGAFEIARRARGTPRIANRLLRRVRDYAEVRADGRITAPVADAALTMLDVDHLGLDLM
                     DRKLLGAMLEKFGGGPVGLDNLAAAIGESSDTIEDVLEPYLIQQGYLQRTPRGRMATA
                     SIWQHFGLKAPGG"
     gene            complement(1037..2101)
                     /locus_tag="PKX01_17100"
     CDS             complement(1037..2101)
                     /locus_tag="PKX01_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ77905.1"
                     /translation="MAPRRTAQIAIIALLLLGCAFVLAPFFAAILFAGTVCITTWPMY
                     AALHRRLGRRDGLAAISMTVLLTAVLLLPMIGLTASLTDAVAEGVDQAREAIEQSDGK
                     PPAWLKDIPVVGPDVDVYVRRLTRNHEEARKLALQALEPARKFALGAARVVGQGLLQI
                     SLVLFIVFFLYRDGERLARMLDTGALRLGGELGERLLELAGNTVKAVMIGIVGTAAAQ
                     ALVALAGFLVAGVPGALLLAAGIFFLSMVPVGPPLIWGGAAFWLYQQDQSGWAIFMVL
                     WGTLAVSSVDNFLKPMLISRTASLPILLIALGAFGGVLAFGFIGLFLGPTFLALGQAL
                     VREWTAIEPQIAAPAAEDEA"
     gene            complement(2197..2688)
                     /locus_tag="PKX01_17105"
     CDS             complement(2197..2688)
                     /locus_tag="PKX01_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMZ77906.1"
                     /translation="MSRKLRQGFLRNVQIGAGSLRLLMNLWPPFIGAGIHVERIAPDF
                     TEAVVRLRLGLLNRNAMGTHFGGSLYAMTDPFFALLVMYQLGRGYVVWDKASRIDYKL
                     PGRGTVYATFRVPPAQVDAIRHATAGGDKVEPVFEVDVLNRRGEVVATVEKTVYVRRR
                     PAD"
     gene            complement(2685..>3131)
                     /gene="ruvA"
                     /locus_tag="PKX01_17110"
     CDS             complement(2685..>3131)
                     /gene="ruvA"
                     /locus_tag="PKX01_17110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMZ77907.1"
                     /translation="LAVREDGHFLYGFASEDERAAFRQLLKVAGVGARTALAVLSGLS
                     VSDLAQAIALQESGRLIKIPGIGKKTAERLLLELRDKLPKALPGLAAAGGLATAAPGD
                     GRSDIVHALLALGYNEKEALAAVKTLPEGVGVSDGIRQALKGLSKA"
BASE COUNT          480 a         1062 c         1160 g          429 t
ORIGIN      
        1 ccgcccggcg ccttcagccc gaagtgctgc cagatggacg cggtggccat gcggccgcgg
       61 ggggtgcgct gcaggtagcc ctgctggatg aggtagggtt ccagcacgtc ctcgatggtg
      121 tcggaggatt cgccgatggc ggcggcgagg ttgtccagac cgaccgggcc gccgccgaac
      181 ttttccagca tcgcgccgag gagcttgcgg tccatcaggt ccaggcccag gtgatccacg
      241 tccagcatgg tcagcgccgc gtcggccacc ggcgcggtga tgcggccgtc ggcgcgcacc
      301 tcggcgtagt cgcgcacgcg gcgcagcagg cggttggcga tgcgcggcgt gccgcgggcg
      361 cggcgggcga tttcgaaggc gccggtgtcc tcgatctgca cgtcgagcag gccggcggaa
      421 cggctgacga tccaggccag ctcttcggcg gtgtagaact ccaggcggga gacgatgccg
      481 aagcggtcgc gcagggggtt ggtgagcatg ccggcgcggg tggtggcgcc caccagggtg
      541 aagggcggca gatccagctt gaccgagcgg gccgccggac cttcgccgat catgatgtcg
      601 atctggaaat cctccagggc cgggtagagg atctcctcca ccaccgggct gaggcggtgg
      661 atctcgtcga tgaacaggac gtcgtggggt tccaggttgg tgaggatcgc cgccagatcg
      721 cccgcccgct ccagcaccgg gccggaggtc tggcgcaggt tgacgcccat ctccgccgcc
      781 acgatgtggg ccagggtggt cttgcccagg cccggcgggc cgaacaggag cacgtggtcg
      841 agggcctcgc tgcggcgccg cgcggcctgg atgaagatct ccagctggtc gcggatcttc
      901 tgctggccca cgtaatccgc cagccgcttg ggccgcaggg cgcgctccag ggcgtcttcc
      961 tgggcgccgt tgctgcgggc gccgatcagc cgcgcggccg gatcgggcgc gccgcccttg
     1021 agcttgtcgg tctcgatcat gcctcgtcct ccgcggccgg cgccgcgatc tgcggctcga
     1081 tggcggtcca ttcccgcacc agcgcctgcc ccagggccag gaaggtgggg ccgaggaaca
     1141 ggccgatgaa gccgaaggcc agcacgccac cgaaggcgcc cagggcgatc agcaggatgg
     1201 gcaggctggc ggtgcggctg atcagcatcg gcttgaggaa gttgtccacc gaactgaccg
     1261 ccagggtgcc ccacagcacc atgaagatcg cccagccgct ctggtcctgc tgatacagcc
     1321 agaaggccgc gccgccccag atcagcggcg gcccgacggg caccatggac aggaagaaga
     1381 tccccgccgc gagcagcagc gcccccggca cgccggccac cagaaagccc gccagcgcca
     1441 ccagggcctg ggccgccgcg gtgccgacga tgccgatcat caccgccttc acggtgttgc
     1501 cggccagttc cagcaggcgc tcgccgagct cgccccccag gcgcagggcg ccggtgtcga
     1561 gcatgcgggc caggcgttcg ccgtcccggt acaggaagaa cacgatgaac agcaccaggg
     1621 agatctgcaa cagcccctgg cccaccaccc gcgcggcgcc cagggcaaac ttgcgcgccg
     1681 gctccagcgc ctgcagggcc agcttgcgtg cctcctcgtg gttgcgggtg aggcggcgca
     1741 cgtacacatc cacgtccggc cccaccacgg gaatgtcctt gagccacgcc ggcggcttgc
     1801 cgtcggactg ctcgatggcc tcccgcgcct gatccacgcc ctccgccacc gcatccgtga
     1861 gggacgccgt cagcccgatc atgggcagca gcagcaccgc cgtgagcagc accgtcatgg
     1921 agatcgccgc cagcccgtca cgccgcccca gccgccgatg cagggcggcg tacatgggcc
     1981 aggtggtgat gcacaccgtc ccggcaaaca ggatcgccgc aaagaacggc gccagcacaa
     2041 aggcacagcc cagcagcagc agggcgatga tggcgatctg ggcggtacgt cggggagcca
     2101 tggggatacg ggggaatggg gaggagggat tatcgctgaa tcgggtgacg gatcggggac
     2161 ggcgatcggc caccgcgcag ccgcacgcgc ccccgcctaa tccgccggcc tgcgcctcac
     2221 gtacaccgtc ttttccaccg tcgcgaccac ctcgccgcgg cggttgagga cgtccacctc
     2281 gaacaccggc tccaccttgt cgccgccggc ggtggcgtgg cggatcgcgt ccacctgcgc
     2341 aggcgggacg cggaaggtgg cgtacacggt gccgcggccg gggagcttgt agtcgatgcg
     2401 cgaggccttg tcccacacca cgtagccacg gccgagctgg tacatgacca gcagggcgaa
     2461 gaaggggtcg gtcatggcgt acagggagcc accgaagtgg gtgcccatgg cgttgcggtt
     2521 gagcaggccc aggcgcaggc gcacgacggc ttcggtgaag tccggggcga tgcgttccac
     2581 gtggatgccg gcgccgatga agggcggcca caggttcatg agcaggcgca ggctgccggc
     2641 gccgatctgc acgttgcgca ggaagccctg gcggagcttg cggctcatgc cttggagagg
     2701 cccttgaggg cctggcggat gccgtcggag acgccgacgc cttcgggcag ggtcttgacc
     2761 gcggcgaggg cttccttctc gttgtagcca agggcgagga gggcgtggac gatgtcgctg
     2821 cgtccgtcgc ccggcgcggc ggtggcgagc ccgccggcgg cggcgaggcc gggcagggcc
     2881 ttgggcagct tgtcgcgcag ctccagcagc aggcgttcgg cggtcttctt gccgatgccg
     2941 gggatcttga tgaggcggcc ggattcctgc agggcgatgg cctgggcgag gtcgctgacc
     3001 gataggccgg agagcacggc cagcgcggtg cgggccccca cgccggccac cttgaggagc
     3061 tggcggaagg cggcgcgctc gtcctcgctg gcaaagccgt agaggaagtg gccgtcctcg
     3121 cgcaccgcca g
//
LOCUS       DAOQPA010000222         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_23334_length_3109_cov_16.000655, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000222 DAOQPA010000000
VERSION     DAOQPA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23334_length_3109_cov_16.000655"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKX01_17115"
     CDS             complement(<1..503)
                     /locus_tag="PKX01_17115"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HMZ77908.1"
                     /translation="MRSLRLFVALLLCCATSAFAAPASEETIRQILSVTNARALAESV
                     LTQMDANISQGINQSLQGKQPNPAQQQAIERFKARMVTTLRDALAWDKLEAMNIRLYK
                     EAFSEEELKGILAFYKTPAGQAVIHKMPTLMAQTMREMQQTMVSVTPALLKIQQEFGE
                     EMKAAAET"
     gene            complement(876..2534)
                     /locus_tag="PKX01_17120"
     CDS             complement(876..2534)
                     /locus_tag="PKX01_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HMZ77909.1"
                     /translation="MPVRLIAPTPSAYAFPLLIKHLLLTPLATVPEQTITYQGRVRYT
                     YRTLKDRIGRLANVLAGLGVEAGQTVAVMDWDSHRYLEAFFAVPMMGAILQTVNVRFS
                     PEQILYTLNHARADVLIVNSEFFAALGEIAGRLETVKTVVTIVEDGKAVALPLAAVGE
                     YEALLAGASADYAFPDFDENAQATTFYTTGTTGHPKGVHFSHRQLVLHTLGCAAALGG
                     ATTQGRVHREDVYMPLTPMFHVHAWGFPYLATMMGLKQVYPGRYQPELICRLVAEEKV
                     SFSHCVPTILHMVMGHPLAKTTDFAGWKILIGGSALPEAMATAAQKRGIDLFTGYGMS
                     ETCPVLTIAHLDSAALAGSAESQSAIRAKTGRPLPLVDLRVVDPQMRDMLHDGKATGE
                     VVVRAPWLTQAYVNAPEASEALWAGGYLHTADIGHIDAAGYLKVTDRIKDVIKTGGEW
                     TSSLELENLIGKHEAVHEVAVIGVPDEKWGERPLALVLLKPEHADTITERALRNWAAH
                     AIEAAGVSRYGVLLQVRFVKTLVKTSVGKMNKRLMRADPDALCL"
BASE COUNT          495 a         1111 c          993 g          510 t
ORIGIN      
        1 gtctccgccg ccgccttcat ctcctccccg aactcctgct ggatcttcag cagtgcgggg
       61 gtcacggaga ccatggtctg ctgcatttcg cgcatggtct gggccatcag ggtcggcatc
      121 ttgtggatta cggcctggcc ggcgggggtc ttgtagaagg cgaggatgcc cttcagttcc
      181 tcttcgctga aggcttcctt gtagaggcgg atgttcatcg cctccagctt gtcccaggcc
      241 agtgcgtcgc gcagggtcgt caccatgcgc gccttgaagc gctcgatggc ctgctgctgg
      301 gccgggttgg gctgtttgcc ctggagggac tggttgatgc cctggctgat gttcgcatcc
      361 atctgggtca gcaccgattc cgccagggcg cgggcgttgg tcaccgacag gatctggcgg
      421 atcgtctcct cggaggcggg cgcggcgaag gcggaggtgg cgcagcacaa cagcagggcg
      481 acaaacaggc ggagggaacg catgggagaa acctcggagg caggcgtgga acggatcgca
      541 ggtccatcgc cgggcggcgc aaacaaagcc ggcgcggcgg cggggtcggg tgaggcagca
      601 ttctggcata gggccggccg cctgtcctgc gcggggggca cgccgggggg acgcacgccg
      661 tgcacgccgc ggcgaggggc gcacaatcct tcccgcgtcg ctgcgcgggc gattcacgcc
      721 cggcggaatg cctcgcgcag ccgcccgagt gcagtgtgga cgccgccgga gccttctccg
      781 ctgcggcggg cgccttcacc gccgacgtcg aatccttccc gcccggccgc gcatccttca
      841 gcgccgtggc accgccgcac gccggctgcg tcccgtcaca gacacagggc gtccggatcg
      901 gcgcgcatca ggcgcttgtt catcttgccc acgctggtct tcaccagggt cttcacgaag
      961 cgcacctgca gcagcacgcc gtagcgcgac acccccgcgg cctcgatggc gtgggccgcc
     1021 cagttgcgca gggcccgctc ggtgatcgtg tccgcgtgct cgggcttgag cagcaccagg
     1081 gcgagggggc gctcgcccca tttttcgtcc ggcacgccga tcaccgccac ctcgtgcacc
     1141 gcctcgtgct tgccgatcag gttctccagc tccagggacg aggtccattc gccgccggtc
     1201 ttgatcacgt ccttgatgcg gtcggttacc ttcaggtaac cggcggcgtc gatgtggccg
     1261 atgtccgccg tgtgcagata gccgcccgcc cacagggcct cggaggcctc cggcgcattc
     1321 acataagcct gggtcagcca cggggcgcgc accaccacct cgccggtggc cttgccgtcg
     1381 tggagcatgt cgcgcatctg cgggtccacc acgcgcagat ccaccagggg cagcggccgc
     1441 cccgtcttgg cgcggattgc ggactgggat tccgccgagc cggccagcgc cgcgctgtcc
     1501 agatgggcaa tcgtcagcac cggacaggtc tccgacatgc cgtagccggt aaagaggtcg
     1561 atgccgcgct tctgcgccgc cgtcgccatc gcctccggca gggccgaacc gccgatcagg
     1621 atcttccagc ccgcaaaatc cgtcgtcttc gccagcggat ggcccatcac catgtgcagg
     1681 atggtcggca cgcagtggga aaagctcacc ttctcctccg ccaccagccg gcagatcagc
     1741 tccggctgat agcgccccgg atacacctgc ttcagcccca tcatcgtcgc cagatacgga
     1801 aagccccagg cgtgcacgtg gaacatgggc gtcagcggca tgtacacatc ctcccgatgc
     1861 acccgcccct gggtcgtggc cccgcccagc gccgccgcac agcccagcgt gtgcagcacc
     1921 agctggcgat ggctgaagtg cacccccttc gggtgccccg tcgtccccgt cgtataaaag
     1981 gtggtggcct gggcgttctc gtcgaaatcg gggaaagcgt agtccgcaga ggcccccgcc
     2041 agcagcgcct cgtattcgcc aaccgcggca aggggcaggg ccaccgcctt gccgtcctcc
     2101 acgatggtca ccaccgtctt caccgtctcc agccgccccg cgatctcgcc gagggccgca
     2161 aagaactcgc tgttcacgat cagcacgtcc gcgcgggcgt ggttgagggt gtacaggatc
     2221 tgctccgggc tgaagcgcac attcaccgtc tgcaggatcg cccccatcat cggcaccgcg
     2281 aagaaggctt ccagataacg gtgactgtcc cagtccatca ccgccaccgt ctgcccggcc
     2341 tccaccccca gcccggccag cacattcgcc agcctgccga tgcggtcctt gagcgtgcgg
     2401 taggtgtacc gcacccgccc ctgataggtg atcgtctgct ccggcaccgt cgccagcggc
     2461 gtgaggagca gatgcttgat cagcagcgga aacgcgtagg cggacggggt gggggcgatg
     2521 aggcggacgg gcatgagggg ctccggcggg cgggattcgg gcgggtattt tacgcctgcg
     2581 tgacgaccgg accggtgtat accaaggcga agtgatgctg tctgccatcc gccgtggggc
     2641 cttgtcacgg cgcattcaag caatcactca agcgtgacgg tctcaccctt gacacgctca
     2701 acaatccccc cggcgccacc gccgacccgg ccttcaccgc cggcaatggc acctacacca
     2761 tcaacctcaa ggtcaaagac ctcaccgtcg acggcaagac ctaccgctac ttcgactccg
     2821 ccaaggccgt ccgcaacgga ttcccggaga tttcacctga tgcatcccat cccccgtcgc
     2881 cgcgggggat ggaaactgcg acgcgccatg cccgccctgg ccgtcgcagt ttccttttct
     2941 gccgcggcca aagatttgag cttcgacacc gtcttccgca cccagggcga accgcccacc
     3001 ctgcacgaca aggccctcgt cctcgccaaa ggccaggaac gccccctcga agtctggcgc
     3061 gaccacgacc gccgcctcaa acgcgtcacc ggcgacgacc tggaggtct
//
LOCUS       DAOQPA010000223         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_23568_length_3092_cov_18.086928, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000223 DAOQPA010000000
VERSION     DAOQPA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23568_length_3092_cov_18.086928"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKX01_17125"
     CDS             complement(<1..425)
                     /locus_tag="PKX01_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HMZ77910.1"
                     /translation="MNPPLIVNERDGSRFAQAPGDTVLRAALRAGIGLSYECNSGGCG
                     GCKIELLQGNVETLWPDAPGLGERDRRRGRHLACQCRAVGDITIKAPTAPEYVPPILP
                     RRRRAFLIGTTDLTHDLREFRFAADGPADFLPGQYAMLDL"
     gene            complement(488..1474)
                     /locus_tag="PKX01_17130"
     CDS             complement(488..1474)
                     /locus_tag="PKX01_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HMZ77911.1"
                     /translation="MSEIQVLKPQKTWSHLAVRRRKPSEYEIVSANLHYNDRDPNAPY
                     ELDPGMFMNEWYKKHTFGTALKHADWNAFRDPDEVVYRTYNMMQDGQETYVFGLFDQF
                     NEREHDKALDPRWAGTLARLYTPARYLFHTLQMASAYVGQVSPASTITNCHYFQMADS
                     LRWLSHTAYRTTELARAFADKGFGRDERHYWEEDAVWQGFRELMEKALTVWDWGEAIV
                     VLNLIVKPAVEETVLRRLGESARHNGDTLLGLLTDAQLIDVARHRRWAAAFVAMALET
                     PGNRELIAGWIAQWEPLADRAIDAYCAALPDVPEAAAAARAATRDLRRSLGF"
     gene            complement(1518..1832)
                     /locus_tag="PKX01_17135"
     CDS             complement(1518..1832)
                     /locus_tag="PKX01_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HMZ77912.1"
                     /translation="MSTAAAGQAYHNNLVGPVMRAGDLAMAVIEAAKVDNPGKDIYVD
                     DKRAYIRIHAEQEMILRQATIEEELGRPFRMNDLEVDLSSFAGQIESRDDSVRFYFVK
                     KL"
     gene            complement(1891..2226)
                     /locus_tag="PKX01_17140"
     CDS             complement(1891..2226)
                     /locus_tag="PKX01_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMZ77913.1"
                     /translation="MCFTKVCTLDDVWEGEMAAFTVNGHEILLVCPEGGEIKAFQGVC
                     PHQDIALAEGKFDGKRVICRAHLWQFDAATGQGVNPDDCALAVYPVKVEGDDVLVSTE
                     GVEPLFAHT"
     gene            complement(2252..2512)
                     /locus_tag="PKX01_17145"
     CDS             complement(2252..2512)
                     /locus_tag="PKX01_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene-4-monooxygenase system B family protein"
                     /protein_id="HMZ77914.1"
                     /translation="MALFPLTSNFEGDFVLQLLPVDTEHTMDEVAAAAAVHSVGRRVR
                     ARPGHILRVRRHGSAEFLPRTLKVGEAGFQPTETVDIIWQAA"
     gene            complement(2569..>3092)
                     /locus_tag="PKX01_17150"
     CDS             complement(2569..>3092)
                     /locus_tag="PKX01_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HMZ77915.1"
                     /translation="HGMHLGSYYWRPTVWWNPAAGVTPDERQWLEEKYPGWNDTWGQC
                     WDVIIDNVVDGNMAKAYPETLPYVCNMCQLPILGTPGKGWNVKDYPLEYKGRLYHFGS
                     EVDRWVFQQEPERYAGHLSIVDRFLAGMIQPMNLEGALAYMNIAPGECGDDAHNYAWA
                     EVYRALRAARKAG"
BASE COUNT          449 a          969 c         1133 g          541 t
ORIGIN      
        1 agatccagca tggcgtactg gccgggcagg aagtcggccg gaccgtcggc ggcaaagcgg
       61 aattcgcgca ggtcgtgggt caggtcggtg gtgccgatca ggaaggcgcg acggcgccgg
      121 ggcaggatcg gcggcacgta ctcgggcgcg gtgggcgcct tgatggtgat gtcgccgaca
      181 gcgcggcact gacaggccag gtgacggccg cggcggcgat cccgctcgcc caggccgggt
      241 gcgtcaggcc acagagtctc cacgttgccc tgcagcagct cgatcttgca gccgccgcag
      301 ccgccggaat tgcattcgta ggacaggccg atgccggccc gcagggcggc gcgcagaacg
      361 gtgtcgccag gggcctgcgc gaaacggctg ccgtcccgct cgttgacgat gaggggtgga
      421 ttcatggcgt ctcccggcgc accgccgcgg cccgacagga ccgcagcggt cgcccgtggc
      481 ggcgggctca gaagcccagg ctgcgacgca ggtcgcgggt ggcggcacgg gccgcggcgg
      541 cggcttcggg cacgtcgggc agggccgcgc agtaggcgtc gatggcccgg tcggccagcg
      601 gttcccactg ggcgatccag ccggcgatga gttcccggtt gcccggcgtc tccagggcca
      661 tggccacgaa ggcggcagcc cagcggcggt ggcgcgccac gtcgatgagc tgggcgtcgg
      721 tgagcaggcc caacagggtg tcgccgttgt ggcgggcgga ttctcccagg cggcgcagca
      781 ccgtctcctc gaccgccggc ttgacgatca ggttgaggac cacgatggcc tcgccccagt
      841 cccacacggt gagggccttt tccatcagct cgcggaagcc ctgccagacg gcgtcctctt
      901 cccaatagtg ccgttcgtcg cgaccgaagc ccttgtcggc gaaggcgcgc gccagctcgg
      961 tggtccggta agcggtgtgg ctcagccagc gcaggctgtc ggccatctgg aagtagtggc
     1021 agttggtgat cgtgctggcg ggcgagacct gccccacgta ggcggaggcc atctgcaggg
     1081 tgtggaagag gtagcgggcc ggcgtataca gacgcgccag ggtgccggcc cagcggggat
     1141 cgagggcctt gtcgtgctca cgctcgttga actggtcgaa gaggccgaag acgtaggtct
     1201 cctggccgtc ctgcatcatg ttgtaggtgc ggtagaccac ctcgtccgga tcgcggaagg
     1261 cgttccagtc ggcgtgcttg agggcggtgc cgaaggtgtg cttcttgtac cactcgttca
     1321 tgaacatgcc cggatccagc tcgtagggcg cgttcgggtc gcggtcgttg tagtgcaggt
     1381 tggcgctgac gatctcgtat tcgctgggct tgcggcggcg caccgccaga tggctccagg
     1441 tcttctgggg cttcaggact tgaatctcgg acatgggtgt ctcctcgtgt ctttttgggc
     1501 gtggacgggg cgggcgctca gagcttcttg acgaagtaga agcgcaccga gtcgtcgcgg
     1561 ctctcgatct ggccggcgaa ggagctgagg tccacctcca ggtcgttcat gcggaagggc
     1621 cggcccagct cctcttcaat ggtggcctgg cgcaggatca tttcctgctc ggcgtggatg
     1681 cggatgtagg cgcgcttgtc atccacgtag atgtccttgc cggggttgtc caccttggcg
     1741 gcctcgatga ccgccatggc cagatcgccg gcccgcatga ccggaccgac caggttgttg
     1801 tgataggcct gacccgctgc tgctgtactc atggtgtctc cttgtgggat gccggggacg
     1861 ccgcgccggg cggcgtccgc aaggtccggc tcaggtgtgg gcgaacaggg gctcgacgcc
     1921 ttcggtgctg accagcacgt cgtcgccctc gaccttgacg gggtagaccg ccagggcgca
     1981 gtcgtcggga ttgacgccct ggccggtggc ggcgtcgaac tgccacagat gggcgcggca
     2041 gatcacccgc ttgccgtcga acttgccttc ggcgagggcg atgtcctggt gcggacagac
     2101 gccctggaag gccttgattt cgccgccctc ggggcagacg aggaggatct cgtgtccatt
     2161 gacggtgaag gccgccatct cgccttccca cacgtcatcg agggtgcata ccttggtgaa
     2221 gcacatgcgc tcgctccgat cgcggtggcc gtcaggcggc ctgccagatg atgtccacag
     2281 tctcggtggg ctggaagccc gcctcgccga ccttcagggt gcgcggcagg aactccgcgc
     2341 tgccgtggcg gcggacccgc aggatgtgac ccggacgggc ccggacgcgg cggccgacgg
     2401 aatgcactgc agcggcggca gccacctcgt ccatggtgtg ctcggtatcc accggcaaca
     2461 gttgcaggac gaaatccccc tcgaaattgg aggtcaatgg aaataaggcc atgcttcgaa
     2521 tctcctcgtg gtgtacggcc ggcccacgcg gggccggcgg tgtcgggctc agccggcctt
     2581 gcgggcggcg cgcagggcgc ggtagacctc ggcccaggcg tagttgtggg catcgtcgcc
     2641 gcattcgccg ggggcgatgt tcatgtaggc gagggcgcct tccaggttca tgggctggat
     2701 cattccggcc aggaagcgat cgacgatgga caggtggccg gcgtagcgct cgggctcctg
     2761 ctggaacacc cagcgatcca cctcggagcc gaagtggtac aggcggccct tgtattccag
     2821 cgggtaatcc ttgacgttcc agcccttgcc cggcgtgccg aggatgggga gctggcacat
     2881 gttgcagacg tagggcagcg tctccgggta ggccttggcc atgttgccgt ccaccacgtt
     2941 gtcgatgatc acgtcccagc actggcccca ggtgtcgttc cagccggggt acttctcttc
     3001 cagccactgc cgctcgtccg gggtgacccc cgcggcgggg ttccaccaca cggtggggcg
     3061 ccagtagtag gagcccaggt gcatgccgtg gt
//
LOCUS       DAOQPA010000224         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_23606_length_3089_cov_25.333883, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000224 DAOQPA010000000
VERSION     DAOQPA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23606_length_3089_cov_25.333883"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKX01_17155"
     CDS             <1..562
                     /locus_tag="PKX01_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77916.1"
                     /translation="VQESAKPWVLALDRPFMEGVATWPDGRFEYRYFGGHHMLQLCLG
                     KLSERDITAFLQGQVHVGFYLRQAVLFVLFKIEHLMDWSDQAFTLHLVTPEDRDLLPH
                     VPGTHQALSLVLVEAESGLVKGMRLVTYSKHASALLHRILQQQLASPFDADRHAALVA
                     EVYRQYPHSRLLAKAAILIEKAGGQQ"
     gene            559..1752
                     /locus_tag="PKX01_17160"
     CDS             559..1752
                     /locus_tag="PKX01_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ77917.1"
                     /translation="MTFPTPLPFPVELARNQALSGPRLTAARNDHEAVATWLAKYTDN
                     RHTLDSYRREAERLMLWATWHDKDLPGLMVEDVLAYKAFLSDPQPVDLWCQQTEPRTL
                     PDGSPNPLYRQVRRVTRRLPDGSANPTWRPFVSGLSASAVKQTTTILFGLFEHLAAIG
                     YVHANPLRAAAKRTRKGQRQGQERYFDAAAWAALKECIEAMPKETAREAAHYHRTRFL
                     FSFLYLTGLRRFEMAKARTSDLRFKRGQWWLEVLGKGSVEGVVPLPADALEAIKSYRE
                     STGRPALPPSGTDEPLLMDLTGAGRSLSDRAIYAIVKEVCRQAANSVAAAQPDLARQI
                     DSGSTHWLRHTSASHQIDRGVDPFIVQRNLRHASFSTTEGYIHKDRDQQHQETEKHTI
                     TDVPS"
     gene            1874..2533
                     /locus_tag="PKX01_17165"
     CDS             1874..2533
                     /locus_tag="PKX01_17165"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ77918.1"
                     /translation="MRPSASQSQLRFPLTRLLGNGGSVRVLRALIAYGGPLSVSQLAH
                     ECCLSRQGTYQTLNSLVSQRLVRVLGQARSQLFTIDTNHPLSPGLKELFGHEQSRWDE
                     LLQVLRDTFQTNKDIDAAWYYGSVARGEDEPHSDLDIAVIAPDGRVDETVESVRQALR
                     EVEDRLYISCSIVGLSPSDVMRLSSGDDWWQNMARDAKVLKGVGPEPYAATLIRQRMK
                     A"
     gene            2533..2943
                     /locus_tag="PKX01_17170"
     CDS             2533..2943
                     /locus_tag="PKX01_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77919.1"
                     /translation="MARISTRKSVDVDYWRGRLEAARAFHKAAEDAITLAEPGSNANP
                     AISQMVLAAIAYGDCLTARRANVINQQSHAAAPKLLRDVLGATLPTAQENRYRRLLGH
                     KDESQYGARSASVIHAQRLLDELQEFALWVEDQF"
BASE COUNT          619 a          969 c          917 g          584 t
ORIGIN      
        1 cgtgcaggaa tctgcaaagc cctgggtgct ggcactcgat cggccattca tggagggcgt
       61 cgccacgtgg cccgatgggc gcttcgaata ccgttacttc gggggtcatc acatgctgca
      121 actgtgcctg ggcaaactga gcgagcggga catcacggct ttccttcagg gtcaggtgca
      181 tgtcggcttc tacctccggc aggcggtgct gttcgtcctg ttcaagatcg agcatttgat
      241 ggactggtct gatcaggctt tcacactcca tctggtgacg ccggaagacc gcgacctcct
      301 gccgcacgtc ccgggcaccc atcaggccct gtcgctggtc ctcgtcgaag ccgaaagcgg
      361 actggtgaag ggcatgcgac tcgtgacgta ctcgaaacac gccagcgcct tgctgcaccg
      421 gatcctgcag caacaactgg catcaccgtt cgacgccgat cgccatgccg ctctggtcgc
      481 tgaggtttac cggcaatatc cacactcccg actgctggcc aaagcggcga ttctgattga
      541 gaaggcaggg ggacaacagt gaccttcccg acccccctgc ccttccccgt cgagcttgcc
      601 cgaaaccagg cactttccgg gccgcggctg acggcagcca ggaacgatca tgaagcagtc
      661 gccacctggc tggcaaagta cacggataac cgccacacgc ttgacagcta tcgccgggag
      721 gccgagcggt tgatgctctg ggccacctgg cacgataagg atctgccggg gctgatggtc
      781 gaggacgtgc tggcctacaa ggctttcctg agcgacccac agccggtgga tttgtggtgc
      841 cagcagaccg agccgcggac gctccctgac ggttcgccga atcccttgta tcggcaagta
      901 cgacgggtga cgcgccggct gcccgatggt tcggcaaacc ccacctggcg tcccttcgtc
      961 tcgggactgt cggcgtcggc cgtgaagcag accacgacga ttctgtttgg tctcttcgag
     1021 cacctggcag ccatcggcta tgtccacgcc aatccgttgc gggcggcagc caagcgtacc
     1081 cggaaaggcc aacggcaagg acaagaacgt tactttgacg cggcagcctg ggcggcgctg
     1141 aaggagtgta tcgaggccat gcccaaggag actgcgcggg aggcggcaca ctaccaccgc
     1201 acccggttcc ttttttcttt cctctacctg accggcctgc ggcgattcga aatggccaag
     1261 gctcgcacgt ctgatcttcg tttcaaacgc ggccaatggt ggcttgaggt gctcggcaag
     1321 ggaagcgtcg agggcgtagt cccgctgccc gcggacgcac tggaagcaat caagtcgtat
     1381 cgggagagta ccggccgccc tgccctgccc ccaagcggca cggatgaacc gctgctgatg
     1441 gacctcaccg gcgccggacg ctcgctctcc gatcgggcga tctacgcgat cgtcaaagag
     1501 gtgtgtcgtc aggcggcgaa cagtgtagcg gccgctcagc ccgatctggc caggcagatt
     1561 gattctgggt cgacgcactg gctgcggcat acctcggcga gccaccagat cgaccggggt
     1621 gtggatccct tcatcgtcca gcgtaacctg cggcacgcga gcttctcaac gactgaaggc
     1681 tacatccaca aggaccgcga ccaacagcat caggaaacgg aaaaacacac gatcaccgat
     1741 gtgccctcgt gaccacgctc tcgtggagcc tcgggacatc tgcgtcgtct cgaccttata
     1801 cccgttttcg ctctcgatag tcaattagcg ttgactagtg acaagtgtaa gtgactttat
     1861 tacactttac ttcatgcgcc cttcagcatc ccagagccag cttcgatttc cactcacccg
     1921 tttgcttggg aacggtgggt ctgttcgagt tttgcgtgct ttaattgcct atggcggccc
     1981 gctcagcgta tcgcaattgg cgcatgagtg ctgcttgtcg cgccaaggaa cctaccagac
     2041 actcaattct ctggtaagcc agcgactcgt gcgcgtactc gggcaggccc gttcccagct
     2101 attcacgatt gacacgaatc atcccctgtc acccggacta aaggagctgt tcggccatga
     2161 gcaatcccga tgggacgaac ttcttcaggt gctgcgcgat acgttccaga ccaacaaaga
     2221 cattgatgcc gcctggtact acggcagtgt ggcacggggc gaggatgagc cccatagcga
     2281 cctggatatc gcggttatcg cgccggatgg ccgggtagac gagacggtgg aatccgtgcg
     2341 gcaagcgttg cgcgaagtcg aggaccggct gtacatcagt tgttcgattg taggcttgtc
     2401 gccatccgac gtcatgaggc tgtccagcgg cgacgactgg tggcagaaca tggctcgcga
     2461 tgccaaggtt ctcaaaggcg tcggtccgga gccgtatgcc gcgacactca tccgccagcg
     2521 tatgaaggcg tgatggcgcg catcagtaca agaaagtccg tcgatgtcga ctattggcgt
     2581 gggcgacttg aagcggctcg cgcctttcac aaagcagccg aggacgccat caccctggct
     2641 gaaccgggat ccaatgccaa tccggcgatc agtcaaatgg tcctggcagc cattgcctac
     2701 ggcgactgct tgacggcaag acgagccaac gtaatcaacc agcaaagcca cgcagccgca
     2761 cccaaactgc tgcgcgacgt gcttggggcg accttaccga ccgcgcagga aaaccgctat
     2821 cgccgcctac tgggtcacaa ggatgaatcc cagtacggcg cacgcagcgc gtcggtcatc
     2881 cacgcccagc gtctccttga tgaacttcag gaatttgcgc tctgggtcga agaccagttt
     2941 taaggtggca aaccggccca cagctcagca agcctgaggc ccctacccgg ggacgcactg
     3001 gaagcgatca agtcctatcg ggacagtacc ggcagccctg ccctgccccc cagcggcacg
     3061 gatgaaccgc tgctgatgga cctcaccgg
//
LOCUS       DAOQPA010000225         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_24212_length_3045_cov_18.031104, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000225 DAOQPA010000000
VERSION     DAOQPA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24212_length_3045_cov_18.031104"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..2139
                     /locus_tag="PKX01_17175"
     CDS             172..2139
                     /locus_tag="PKX01_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ77920.1"
                     /translation="MSVTPGHDDAALETFPRLLMQHAALRPKRPAMREKAYGIWQTYT
                     WAEVAERVCALAAGLAELGFKRGDRLAVVGDNRPRLYWSVAACQCLGGIPVMMYQDAV
                     AQEMAYVMQDAEIRFACVEDQEQVDKMLEIQPGLPQLEHVIYDDPRGLRHYNQPFLHG
                     LDEVLEMGRIHTEHHPDFIAGEIGKGSADDVSVMLYTSGTTGKPKGVCQTHAAFIAAA
                     RGGCRFDRLSDREDILSYLPMAWVGDHLFSYAQALVAGFTINCPESGDTVMGDLREIG
                     PTYYFAPPRVFENLLTQVMIRMEDAGGFKRALFAHFMDVAKRCGADLLDGKPVGMGDR
                     LQYALGDWLIYGPLKNVLGMSRIRVAYTAGAAIGPDLFRFYRSIGINLKQLYGQTETC
                     AYVCLQPDGQIKLDSVGTPAPDVEVKLADNGEILVRGPMLLKAYYRRPEATAESLNAE
                     GYFMTGDAGFFDEDGHLKIIDRAKDVGKLTGGGMFAPNYIENKLKFFPYVKEAVCFGN
                     GRDFVTAFINIDLEAVGNWAERRGLAYAGYTDLAGQDAVYGLIRESVEQVNADLATDA
                     KMGDSQVRRFLILHKELDADDGELTRTRKVRRNFIAEKYALLIDALYAGKARQYIETQ
                     ATYEDGRTHSVAADLRIEDAATYPPQPRKAA"
     gene            2151..2933
                     /locus_tag="PKX01_17180"
     CDS             2151..2933
                     /locus_tag="PKX01_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77921.1"
                     /translation="MARQTGDVIVDLQNISLRFGGVKALTDISFNVREHEIRSIIGPN
                     GAGKSSMLNVINGVYHPQEGQIFFRGRLRKKMEPHQAAEQGIARTFQNIALFKGMTVL
                     DNIMTGRTLKMRCNFLQHALYWGAAQKEEIAHRIKVEEVIEFLEIQDIRKTPVGRLPY
                     GLQKRVELARALAAEPHILLLDEPMAGMNVEEKQDMCRFILDVNDHYGTTIVLIEHDM
                     GVVMDISDRVVVLDYGKKIGDGEPAEVKNNPDVIRAYLGTAH"
BASE COUNT          565 a          992 c         1050 g          438 t
ORIGIN      
        1 acaattccgt acatttgaaa aacagttgag caaacatgga ctgcgacact cctttccact
       61 gaattccccc cgagcgccgc gggccgtgag gccggcggcg ggacgcggag gccggacgcg
      121 ataaccgggc cccgcgggcg gaatggcggt ggagacgagg aggaggagcg aatgtccgtg
      181 accccggggc acgacgacgc ggcgctggag acctttccgc gcctgctgat gcagcacgcg
      241 gcgctgcggc cgaagcggcc ggcgatgcgc gagaaggctt acggcatctg gcagacctac
      301 acctgggccg aggtggcgga gcgggtgtgc gccctggcgg ccgggctggc ggagctgggc
      361 ttcaagcgcg gcgaccggct ggcggtggtg ggcgacaacc ggccgcggct gtactggtcg
      421 gtggcggcct gccagtgcct tggcggcatt ccggtgatga tgtaccagga cgccgtggcc
      481 caggagatgg cctacgtgat gcaggacgcg gagatccgct tcgcctgcgt cgaggaccag
      541 gagcaggtgg acaagatgct ggagatccag cccggcctgc cccagctcga acacgtgatc
      601 tacgacgatc cccgcggcct gcgccattac aaccagccct tcctgcacgg tctggacgag
      661 gtgctggaga tgggccgcat ccacaccgag caccatccgg acttcatcgc cggggagatt
      721 ggcaagggca gcgccgacga tgtgtcggtg atgctgtaca cctccggcac cacgggcaag
      781 cccaagggcg tgtgccagac ccacgcggcc ttcatcgccg cggcgcgggg cggctgccgc
      841 ttcgaccgtc tgagcgaccg ggaagacatc ctctcctacc tgcccatggc ctgggtgggg
      901 gatcacctgt tctcctacgc ccaggcgctg gtggcgggct tcacgatcaa ctgcccggaa
      961 tcgggcgaca cggtgatggg cgacctgcgg gagatcggcc ccacctatta ctttgcgccg
     1021 ccgcgggtgt tcgagaacct gctcacccag gtgatgatcc gcatggagga cgcgggcggc
     1081 ttcaagcgcg cgctcttcgc ccacttcatg gacgtggcga agcgctgcgg ggcggatctg
     1141 ctggacggca agccggtggg gatgggggat cgcctgcaat acgcgctggg cgactggctg
     1201 atctacggac ccctcaagaa cgtgctgggc atgagccgca tccgcgtggc ctacacggcc
     1261 ggcgcggcca tcgggccgga cctgttccgc ttctaccgtt ccatcggcat caacctgaag
     1321 cagctctacg gccagaccga gacctgcgcc tacgtgtgcc tgcagccgga cggccagatc
     1381 aagctggatt cggtgggcac gccggcgccg gacgtggagg tgaagctggc cgacaacggc
     1441 gagatcctgg tgcgcggccc gatgctgctc aaggcctact accggcgccc ggaggcgacc
     1501 gcggaatccc tcaacgccga gggttacttc atgaccggcg acgcgggctt cttcgacgag
     1561 gatggccatc tgaagatcat cgaccgggca aaggacgtgg gcaagctcac cggcggcggc
     1621 atgttcgcgc ccaactacat cgagaacaag ctgaagttct tcccctacgt gaaggaggcg
     1681 gtgtgcttcg gcaacggccg cgacttcgtg acggccttca tcaacatcga cctggaggcc
     1741 gtgggcaact gggccgagcg gcgcgggctg gcctacgcgg gctacaccga tctggccggg
     1801 caggacgcgg tgtacggcct gatccgcgag agcgtggagc aggtgaacgc ggacctggcg
     1861 acggacgcga agatgggcga ttcccaggtg cgccgcttcc tgatcctgca caaggagctg
     1921 gacgcggacg acggcgagct gacccgcacg cgcaaggtgc gccgcaactt catcgccgag
     1981 aagtacgcgc tgctcatcga cgccctgtat gccggcaagg cgcgccagta catcgagacc
     2041 caggcgacct acgaggacgg ccgcacccac agcgtggcgg ccgatctgcg catcgaggac
     2101 gccgccacct atccgcccca gccgcggaag gccgcctgag gagcgcgacc atggcccgac
     2161 agactggcga tgtgatcgtc gacctgcaga acatctccct gcgctttggc ggcgtgaagg
     2221 cgctcaccga catcagtttc aacgtccgcg agcacgagat ccgctccatc atcggcccca
     2281 acggtgccgg caagagctcc atgctcaacg tgatcaacgg ggtgtatcac ccccaggaag
     2341 ggcagatctt cttccgcggc cggctgcgca agaagatgga gccccaccag gcggcggagc
     2401 agggcatcgc ccgcaccttc cagaacatcg ccctcttcaa gggcatgacg gtgctcgaca
     2461 acatcatgac cgggcgcacc ctcaagatgc gctgcaactt cctgcagcac gccctctact
     2521 ggggcgcggc gcagaaggag gagatcgccc accgcatcaa ggtggaggag gtcatcgagt
     2581 tcctcgagat ccaggacatc cgcaagacgc cggtcgggcg cctgccctac ggcctgcaga
     2641 agcgcgtgga gctggcccgc gccctggccg ccgagcccca catcctgctg ctggacgagc
     2701 ccatggcggg catgaacgtg gaggagaaac aggacatgtg ccgcttcatc ctcgacgtga
     2761 acgaccacta cggcaccacc atcgtcctca tcgagcacga catgggcgtg gtgatggaca
     2821 tctcggaccg ggtggtggtg ctggactacg gcaagaagat cggcgacggc gagcccgccg
     2881 aggtgaagaa caatccggac gtgatccgcg cctacctggg cacggcgcac tgacggacgg
     2941 gagcgcggcc ggtgcgcgac ggctgtccgg cgcacgctgc atccagcggc aagaggcagt
     3001 gaaacgaggc ggacggccaa ggccgatcgg cccatgaaga caagg
//
LOCUS       DAOQPA010000226         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_25221_length_2977_cov_14.877823, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000226 DAOQPA010000000
VERSION     DAOQPA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25221_length_2977_cov_14.877823"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKX01_17185"
     CDS             complement(<1..878)
                     /locus_tag="PKX01_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMZ77922.1"
                     /translation="MDRHDLVIVGAGPVGMALALALKDAGLDILLVDARARGAARHDP
                     RVLALAHGTRLTLERLGVWTQLRATPIETIHVSQQGGFGRSLLRATDYEQPALGYVVA
                     AGDLAAALDDALLAARIPVRGDASVSGLAAGADDVLVSLADGHTLSARLAACAEGAIQ
                     GDEDDTLVERDYGQHAVISVATPAGSHGHRAWERFTPQGPLAVLPCGHDCAIVHTAAP
                     DAADALMTLDDAAYLAHLQAHFGQRLRFAAVGPRARYPLRLRYRPNPVGHRTVWLGNA
                     AQTLHPVAGQGYNLALR"
     gene            complement(880..2196)
                     /locus_tag="PKX01_17190"
     CDS             complement(880..2196)
                     /locus_tag="PKX01_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HMZ77923.1"
                     /translation="MSLDITPFQARRQRLLATLQASGGGVAILPTAPERLRNRDAHYG
                     YRFDSYFYYLTGFREPEAVLVLVAGDTPRSLLFCRNKDVEREIWDGFRFGPEAARDSF
                     GFDEAYAIGDLDDQLARLLADQPAIFHSVGYEPDWDSRIAAALNRVRAQARSGARAPE
                     QIRDIRSLIDEMRLVKDDAELALMRRAADISGAAHRRAMRTARPGTREYEVEAELLHE
                     FRRGGSQAPAYTSIVASGANACVLHYVENDRVMQNGELLLIDAGCELDGYASDITRSF
                     PVNGRFSGPQKAAYELVLAAQAAAIAEIRPGASWNAGHDAAVRVLAQGFIDLGLLAGS
                     LDGVLETGSYRQFYMHRTGHWLGLDVHDAGDYKLAGEWRPLVAGNVLTVEPGCYIRPA
                     DGVPEAFWNIGIRIEDDALVTPDGCEILTTAAPKSVADIEALMADR"
     gene            complement(2264..2953)
                     /locus_tag="PKX01_17195"
     CDS             complement(2264..2953)
                     /locus_tag="PKX01_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ77924.1"
                     /translation="MILAAGRGERMRPLTDTCPKPLLAAGGKPLIVWHIERLATAGIT
                     ELVINHAHLGAMIEAALGDGRRLGVTIHYSPEGSALETAGGIARALPLLGDAPFLVIN
                     GDVFCDADFAPLTRAAAGLRADGPLAHLLLVPNPPHHPDGDFGLHADGHIRDDGSGTR
                     LTFAGVGAYHPALFAGVDPDRPAKLAPLLRTAMSDGRVRGTRFDGRWVDVGTPARLRE
                     LDQALRTGTPD"
BASE COUNT          405 a          905 c         1250 g          417 t
ORIGIN      
        1 cgcagggcca ggttgtagcc ctggccggcg accgggtgga gggtctgcgc ggcgttgccg
       61 agccagacgg tgcggtggcc caccgggttc ggccggtagc gcaggcgcag cggatagcgg
      121 gcccgcggac cgacggcggc aaaacgcaga cgctggccga agtgcgcctg caggtgcgcc
      181 agataggccg cgtcgtcgag ggtcatcagg gcgtcggccg cgtcgggtgc cgcggtgtgg
      241 acgatggcgc agtcgtgacc gcagggcagg acggcgaggg ggccctgcgg ggtaaagcgt
      301 tcccaggccc ggtggccgtg gctgccggcg ggcgtggcga ccgagatcac cgcgtgctgg
      361 ccgtagtccc gctccaccag ggtgtcgtcc tcgtcgccct ggatggcgcc ttcggcgcag
      421 gcggcgaggc gggccgacag ggtatggccg tcggcgaggc tgacgagcac gtcgtcggcg
      481 ccggcggcca ggccgctcac gctggcgtcg ccgcgcaccg ggatgcgcgc ggcgagcagg
      541 gcatcgtcga gggccgcggc cagatcaccg gcggcgacca cgtagccgag ggcgggctgt
      601 tcatagtcgg tggcgcgcag caggctgcgc ccgaagccgc cctgctggga gacgtggatg
      661 gtctcgatgg gggtggcccg caattgtgtc cagacgccca gccgttcgag ggtgaggcgg
      721 gtgccgtggg cgagggcgag gacgcgggga tcgtgccggg ccgcgccgcg ggcgcgcgcg
      781 tccaccagga ggatgtcgag gccggcgtcc ttgagggcca gggcgagggc catgcccacc
      841 gggccggcgc cgacgatgac gaggtcgtgg cggtccatgt cagcgatcgg ccatcagggc
      901 ttcgatgtcg gcgacggact tgggggccgc ggtggtgagg atctcgcagc cgtcgggcgt
      961 cacgagggcg tcgtcctcga tgcggatgcc gatgttccag aaggcttccg gcacgccgtc
     1021 ggcgggacgg atgtaacagc cgggctcgac ggtgagcacg ttgccggcca ccagcgggcg
     1081 ccattcgccg gcgagcttgt agtcgccggc gtcatgcacg tcgaggccca gccagtggcc
     1141 ggtgcggtgc atgtagaact gccggtagct gccggtttca aggacgccgt cgaggctgcc
     1201 ggcgagcagg cccaggtcga tgaagccctg ggcgagaacg cgcacggcgg cgtcgtggcc
     1261 ggcgttccag ctggcgccgg gacggatctc ggcgatggcg gcggcctggg cggcgaggac
     1321 cagctcgtag gcggccttct gagggccgct gaagcggcca ttcacgggaa agctgcgggt
     1381 gatgtccgag gcgtagccgt ccagctcgca gccggcgtcg atgaggagca gctcgccgtt
     1441 ctgcatgacg cggtcgttct cgacatagtg cagcacgcag gcgttggcgc cgctggcgac
     1501 gatggaggtg taggccgggg cctggctgcc gccgcggcgg aactcgtgga ggagttcggc
     1561 ctcgacctcg tattcccggg tgccgggtcg ggcggtgcgc atggcgcggc ggtgggcggc
     1621 gcccgagatg tcggcggcgc ggcgcatgag cgccagttcg gcatcgtcct tgacgaggcg
     1681 catctcgtcg atgaggctgc ggatgtcgcg gatctgctcc ggcgcgcggg cgccgctgcg
     1741 ggcctgggcg cggacccggt tgagggcggc ggcgatgcgg ctgtcccagt cgggctcgta
     1801 gccgacggag tggaagatgg cgggctggtc ggcgaggagg cgggcaagct ggtcgtccag
     1861 atcgccgatg gcgtaggcct cgtcgaagcc gaagctgtcc cgcgccgcct cggggccgaa
     1921 gcggaaaccg tcccagatct cccgctccac atccttgttg cggcagaaca gcaggctgcg
     1981 cggggtgtcg cccgccacca gcaccagtac cgcctcgggc tcgcggaagc cagtcaggta
     2041 gtagaaatag ctgtcgaagc ggtagccgta gtgggcgtcc cggttgcgca ggcgctcggg
     2101 cgcggtgggc aggatggcga cgccgccgcc gctggcttgc agggtggcca gcaggcgttg
     2161 gcgacgggcc tggaaggggg tgatgtcgag ggacatggga cgggccggga aggacgggga
     2221 tgaaggatgg accggggcgc gggccgttgg ttgcgagctt acgtcaatcc ggcgtcccgg
     2281 tgcgcaaggc ctggtcaagt tcgcgcaggc gcgccggtgt gcctacgtcc acccagcggc
     2341 cgtcgaagcg ggtgccgcgg acgcgaccgt cggacatggc ggtgcgcagc agcggggcga
     2401 gtttggcggg gcggtcggga tcgacgccgg cgaacagggc cgggtggtac gcgccgacgc
     2461 cagcgaaggt gaggcgcgtg ccgctgccgt cgtcgcggat gtggccgtcc gcgtgcaggc
     2521 cgaagtcgcc gtccggatgg tggggcgggt tgggcaccag gagcaggtgg gcgaggggac
     2581 cgtcggcgcg cagcccggct gcggcacggg tcaggggcgc gaaatccgca tcgcagaaga
     2641 cgtcgccgtt gatcaccagg aagggcgcgt cacccagcag gggcagggct cgggcgatgc
     2701 cgccggcggt ttccagtgcg ctgccttcgg gcgaatagtg gatcgtgacg cccaagcggc
     2761 ggccgtcacc gagggcggct tcgatcatgg cgcccagatg ggcgtggttg atgacgagct
     2821 cggtgatgcc ggcggtggcg aggcgttcga tgtgccacac gatcagcggc ttgccccccg
     2881 ccgccaggag cggcttgggg caggtgtcgg tgagggggcg catgcgttcg ccgcggccgg
     2941 cggcgaggat catcgctttc atggttcaga aggaata
//
LOCUS       DAOQPA010000227         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_25919_length_2932_cov_17.807438, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000227 DAOQPA010000000
VERSION     DAOQPA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25919_length_2932_cov_17.807438"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1164)
                     /locus_tag="PKX01_17200"
     CDS             complement(4..1164)
                     /locus_tag="PKX01_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ77925.1"
                     /translation="MTRFARRMADIQPFHVMEILKRAHELAAQGRDIIHLEVGEPDFP
                     SPKPVVEAARCFLNGGHVHYTPALGLSALREAIAGFYRDRFGADVAPERIIVTPGASG
                     ALMLALAVLTDPGDAWLLPDPGYPSNRHLVRSFEGVPVALPVDATTRFQPTPTQVAEA
                     WTTQTRGLMVATPANPTGTLLTPGEIVALHAAVTARHGVLIVDEIYQGLCYGVDATTA
                     LTRLDDVFVVNSFSKYFGMTGWRLGWLVAPPDYVRPLEKLAQHFFIAPSTPAQHAALA
                     AFRPDTLAILEARREAFAQRRNTLLPALQTLGFRFATEPQGAFYLYADITHLADDSQQ
                     LARRLIEEAGVATTPGIDFGDHGPERHLRIAYTTDENRLLEAAARIRRVLGL"
     gene            complement(1247..>2932)
                     /locus_tag="PKX01_17205"
     CDS             complement(1247..>2932)
                     /locus_tag="PKX01_17205"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HMZ77926.1"
                     /translation="AGMLIKLNPEKRKNSYLAWSDPSDVARVEDRTFICSQNKGDAGP
                     TNNWEDPAKMRKTLNELFKGCMHGRTLYVIPFSMGPLGSPIAHIGVEISDSPYVVTNM
                     RTMTRMGKAVYDVLGADGEFVPCVHTVGAPLGQGEKDSRWPCNPDTKYIVHYPETREI
                     WSYGSGYGGNALLGKKCFALRIASNMARDEGWLAEHMLILGVESPQGEKTYVAAAFPS
                     ACGKTNFAMLIPPRSFDGWKIYTVGDDIAWIKPRTDADGVTRFYAINPEAGYFGVAPG
                     TSEKTNFNAMATLKENIIFTNVALTDDGDVWWEGMSKEAPAHLIDWQGKDWTPELAKE
                     TGRKAAHPNARFTAPAAQCPSIDPNWENPEGVPISAFIFGGRRATTVPLVYQAFNWNF
                     GVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCGYHMGDYFNHWLKMGHAVDQTPGI
                     FCVNWFRMNEKGEFMWPGFGENMRVLKWIVDRVRGRVAAQENALGWMPQYEDIDWTGS
                     DVTKAEFEALTAIDADAWKTELASHKEWFDKLQDRLPKQFVLKRELFELALSV"
BASE COUNT          464 a          849 c         1100 g          519 t
ORIGIN      
        1 tctttaaagg ccgagtacgc ggcggatgcg cgcggcggct tccagcagac ggttctcgtc
       61 cgtcgtatag gcgatgcgca ggtggcgctc cggcccgtgg tcgccgaagt cgatacccgg
      121 cgtggtggcg acgccggcct cctcgatcag gcgccgggca agctgctgac tgtcgtccgc
      181 caggtgggtg atgtcggcgt acagatagaa ggcgccctgg ggctcggtgg cgaagcggaa
      241 tccgagtgtc tgcagggctg gcagaagggt gttgcgacgc tgcgcgaagg cctcgcggcg
      301 ggcttccaga attgccaggg tgtcgggccg gaaggccgcc agagccgcgt gctgggccgg
      361 tgtggagggc gcgatgaaga agtgctgggc cagcttctcc agcgggcgca cgtagtccgg
      421 cggcgcgacc agccagccca ggcgccaacc ggtcataccg aaatacttgg agaaactgtt
      481 caccacgaag acgtcgtcaa ggcgggtgag ggcggtggtg gcgtcgacgc catagcagag
      541 gccctggtag atctcgtcca cgatcaatac gccatggcgg gccgttacgg ccgcgtggag
      601 ggcgacgatc tcgcccggcg tgagcagggt gcccgtggga ttggccggcg tggcgaccat
      661 caggccgcgg gtctgcgtcg tccaggcttc agccacctgg gtgggcgtag gctggaagcg
      721 ggtcgtcgca tccaccggca gggcgaccgg taccccttcg aagctgcgca ccaggtggcg
      781 gttggacgga tagcccggat cgggcagcag ccaggcgtcg ccggggtcgg tgagcacggc
      841 gagggccagc atcagggcgc cggaggcgcc gggggtgacg atgatgcgct cgggcgccac
      901 gtcagccccg aagcgatccc ggtagaagcc ggcgatggct tcgcggaggg cgctcagccc
      961 cagcgccggc gtgtaatgga catgaccgcc gttgaggaag cagcgggcgg cctcgacgac
     1021 cggtttcggg ctgggaaaat ccggttcgcc gacctccagg tggatgatgt cgcgcccctg
     1081 ggcggccagt tcgtgggcgc gcttgaggat ctccatcacg tggaagggct ggatgtcggc
     1141 catgcgacgg gcgaagcggg tcatcgggat ttccttcgca aagcaaaaaa gcgccgattg
     1201 ctcggcgctt tttcgggtac ggcaacggcc tgacggccgg acaagcttag accgacaggg
     1261 ccagctcgaa cagttcgcgc ttgagcacga actgcttggg caggcggtcc tgcagcttgt
     1321 cgaaccattc cttgtgggag gccagctcgg tcttccaggc gtcggcgtcg atggcggtga
     1381 gggcctcgaa ctcggccttg gtcacgtcgg aaccggtcca gtcgatgtcc tcgtactggg
     1441 gcatccagcc gagggcgttt tcctgggcgg cgacgcgacc gcggacacgg tccacgatcc
     1501 acttgaggac gcgcatgttc tcgccgaagc cgggccacat gaattcgccc ttctcattca
     1561 tgcggaacca gttgacgcag aagatgcccg gggtctggtc caccgcgtga cccatcttca
     1621 gccagtggtt gaagtagtcg cccatgtggt agccgcagaa gggcagcatg gcgaagggat
     1681 cgcgacggac cacgccctgg gcgccgaagg cggcggcggt ggtttcggag ccgagggtgg
     1741 cggccatgta cacgccgaag ttccagttga aggcctggta caccagcggc acggtggtgg
     1801 cacggcggcc gccgaagatg aaggcggaga tgggcacacc ttcggggttt tcccagttcg
     1861 ggtcgatgga cgggcactgg gcggccgggg cggtgaagcg ggcgttcggg tgcgctgcct
     1921 tgcggccggt ttccttggcc agctcggggg tccagtcctt gccttgccag tcgatcaggt
     1981 gggccggggc ttccttggac atgccttccc accacacgtc gccgtcgtcg gtcagggcga
     2041 cgttggtgaa gatgatgttt tccttcagcg tcgccatggc gttgaagttg gtcttttcgg
     2101 acgtgccggg ggcgacgccg aagtagccgg cttcagggtt gatggcgtag aagcgggtca
     2161 cgccgtccgc gtcggtgcgg ggcttgatcc aggcgatgtc gtcgccgacg gtgtagatct
     2221 tccagccgtc gaaggagcgc ggcgggatca gcatggcgaa gttggtcttg ccgcaggcgg
     2281 aggggaaggc ggcggcgacg taggtctttt cgccttgcgg gctttccacg ccgaggatca
     2341 gcatgtgttc ggccagccag ccttcgtcgc gggccatgtt ggaggcgatg cgcagggcaa
     2401 agcacttctt gcccagcagc gcgttgccgc cgtagccaga gccgtaggac cagatctcgc
     2461 gggtttcggg gtaatggacg atgtacttgg tgtccgggtt gcagggccag cgggaatcct
     2521 tctcgccctg gccgagggga gcgcccacgg tatgcacgca ggggacgaac tcgccatcgg
     2581 cgcccagcac gtcatagacg gccttgccca tgcgcgtcat ggtgcgcatg ttggtcacca
     2641 cgtaggggct gtcggagatc tccacgccaa tgtgggcgat gggagagccg agggggccca
     2701 tggagaacgg gatgacgtac agggtgcggc cgtgcatgca gcccttgaac agttcgttca
     2761 gggtcttgcg catcttggcc gggtcttccc agttgttggt ggggccggcg tcgcccttgt
     2821 tctgggagca gatgaaggtg cggtcttcga cacgggccac gtccgagggg tcggaccagg
     2881 cgaggtagga attcttgcgc ttttccgggt tcagcttgat cagcatcccg gc
//
LOCUS       DAOQPA010000228         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_26236_length_2913_cov_20.893632, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000228 DAOQPA010000000
VERSION     DAOQPA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26236_length_2913_cov_20.893632"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="PKX01_17210"
     CDS             <1..2292
                     /locus_tag="PKX01_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HMZ77927.1"
                     /translation="NEDVVIPLPRMGDYCDGIERINIELSIQNKLALCEALRAFLEGE
                     LPLHTGDATISREELLGDRRRDALNYLEAVRLRWEWLLENLDLPLAEAEPRFVSYGVV
                     AGELTNRAARPRLFHRLQDYSVRVSWKSELKPRLEKIFDGQMFAPVVEQLRAIHKQVL
                     RGRVFVALHMHAGDGNVHTNIPVNSDNYEMLQTANRAVDRIMALARALDGVISGEHGI
                     GITKLDYLTDAEMANFWAYKQQVDPEGRFNRGKLMRHGALTGNLDNAYTPSFNLMGHE
                     SLIMEQTAIGEISHDIKDCLRCGKCKPVCSTHVPRANLLYSPRNKILSTSLLIEAFLY
                     EEQTRRGVSLAHWAEFEDVADHCTVCHKCEKPCPVDIDFGDVSIKMRNLLRQQGKRSF
                     NPGKAAAMAFLTAKDPATIKTIRAGMIGLGYKAQRLGHQLGKSLGLVQGQVAHPPATL
                     GKPPLKAQVIHFINKPMPGGLPKRTSRALLDIEDDTIVPVIRDPRQLRDDSEAVFYFP
                     GCGSERLFSQVGLATQAMLYHVGATTVLPPGYLCCGYPQTSAGDEDKGQRITTDNRVL
                     FHRVANTLNYLDIKTVIVSCGTCMDQLLKYQFEKIFPGCRLLDIHEYLLEKGLKLDGV
                     QGARYMYHEPCHTPMKVHAGLQVANTLMGTRVDLSDRCCGESGTLAVTRPDISTQVRF
                     RKQEEIEKGAARLRGDDSAAPVKILTSCPSCLQGLSRYAHDAGGVEADYIVVEMARHL
                     LGDNWMADYVDKANQGGIERVLL"
     gene            complement(2383..>2913)
                     /locus_tag="PKX01_17215"
     CDS             complement(2383..>2913)
                     /locus_tag="PKX01_17215"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HMZ77928.1"
                     /translation="AGKPLARNLKARVQTTALGQFPFASVVGCIDSRAAPEIVFDQSI
                     GDLFVARVAGNTVNEDILGSLEYAAKVAGSRVIVVLGHTSCGAIKGACDDVKLGNLTT
                     LLAKFKPAVAAATTPGERTSHNHAFVDEVTELNVKQVVQKIRADSPILKGLEDEGRIR
                     IVGALYDTSSGKVRWY"
BASE COUNT          496 a         1051 c          916 g          450 t
ORIGIN      
        1 aacgaggacg tggtgatccc gctgccgcgc atgggcgact actgcgacgg catcgagcgc
       61 atcaacatcg agctgtccat ccagaacaag ctggccctgt gcgaggccct gcgcgccttc
      121 ctggaaggcg agctgccgct gcacacgggt gatgccacca tctcccgcga ggagctcctc
      181 ggcgaccgcc gccgcgacgc cctcaactac ctggaggccg tgcgcctgcg ctgggaatgg
      241 ctgctcgaga acctcgacct gcccctcgcc gaggccgagc cacgcttcgt ctcctacggc
      301 gtggtggccg gtgaactcac caaccgggcg gcccggccgc gcctgttcca ccgcctgcag
      361 gactactccg tgcgggtctc ctggaagagc gagctgaagc cccgcctgga aaaaatcttc
      421 gatggccaga tgttcgcgcc ggtggtcgag cagctgcgcg cgatccacaa gcaggtgctg
      481 cgcggccgcg tcttcgtggc cctgcacatg catgccggcg acggcaacgt gcacaccaac
      541 atcccggtca actccgacaa ctacgagatg ctccagaccg ccaaccgggc ggtggaccgc
      601 atcatggccc tggcccgcgc cctcgacggc gtcatctccg gtgagcacgg catcggcatc
      661 accaagctgg actacctcac cgacgcggag atggccaact tctgggccta caagcagcag
      721 gtggatccgg agggccgctt caaccggggc aagctgatgc gccacggtgc cctcaccggc
      781 aacctggaca acgcctacac gcccagcttc aacctgatgg gccacgagtc cctgatcatg
      841 gagcagaccg ccatcggcga gatctcccac gacatcaagg actgcctgcg ctgcggcaag
      901 tgcaagccgg tgtgctccac ccacgtgccc cgcgccaatc tgctctacag cccgcgcaac
      961 aagatcctca gcacctccct gctgatcgag gccttcctct acgaggagca gacccgccgc
     1021 ggcgtgtccc tcgcccactg ggccgagttc gaggacgtgg ccgaccattg caccgtctgc
     1081 cacaagtgcg aaaagccctg cccggtggac atcgacttcg gtgatgtctc catcaagatg
     1141 cgcaacctcc tgcgccagca gggcaagcgc agcttcaatc cgggcaaggc ggcggccatg
     1201 gccttcctca ccgccaagga tccggccacc atcaagacca tccgcgcggg catgatcggc
     1261 ctcggctaca aggcccagcg cctcggccac cagctcggca agtccctcgg cctggtgcag
     1321 ggacaggtcg cccatccgcc cgccaccctc ggcaagccgc cgctgaaggc ccaggtgatc
     1381 cacttcatca acaagcccat gcccggcggc ctgcccaagc gcacgtcccg cgccctgctg
     1441 gacatcgagg acgacaccat cgtcccggtg atccgtgatc ccaggcagct gcgcgacgac
     1501 agcgaggcgg tgttctactt ccccggctgc ggttccgaac gcctgttcag ccaggtgggg
     1561 ctggccaccc aggccatgct ctaccatgtg ggcgccacca ccgtgctgcc gcccggctac
     1621 ctgtgctgcg gctatccgca gacctccgcc ggcgacgagg acaagggtca gcgcatcacc
     1681 accgacaacc gggtgctctt ccaccgggtc gccaacaccc tgaactatct ggacatcaaa
     1741 acggtgatcg tgtcctgcgg cacctgcatg gatcagctcc tgaagtacca gttcgagaag
     1801 atcttcccgg gctgccgcct gctggacatc cacgaatacc tgctcgagaa gggcctcaag
     1861 ctcgacggcg tgcagggcgc ccgctacatg taccacgagc cctgccacac ccccatgaag
     1921 gtccatgccg gcctccaggt ggccaacacg ctgatgggca cgcgggtcga tctgtccgac
     1981 cgctgctgcg gcgagtccgg caccctcgcg gtgacacgcc cggacatctc gacccaggtg
     2041 cgcttccgca agcaggagga gatcgagaag ggcgccgccc ggctgcgggg cgacgacagc
     2101 gccgcgccgg tgaagatcct cacctcgtgc ccgagctgcc tgcagggcct gtcccgctac
     2161 gcccacgacg cgggcggcgt ggaggcggac tacatcgtcg tcgagatggc ccgccacctc
     2221 ctcggcgaca actggatggc cgactacgtt gacaaggcca accagggtgg catcgagcgc
     2281 gtgctgctgt aagcggcttt ccgctccgtg acgtgaagag gcccgctgcg cgcgggcctc
     2341 ttcgttctca ggtacgccgg aggcggggtc cggcgcgggc gctcagtacc agcgcacctt
     2401 gcccgagctg gtgtcgtaga gggcgccgac gatgcggatc ctgccctcgt cctccaggcc
     2461 cttgaggatg gggctgtcgg cgcggatctt ctgcaccacc tgcttcacgt tcagctccgt
     2521 cacctcgtcc acgaaggcgt ggttgtggga cgtgcgttcg cccggcgtgg tggccgccgc
     2581 cacggccggc ttgaacttgg ccagcagggt ggtcaggtta cccagcttca cgtcgtcgca
     2641 ggcgcccttg atggcgccgc aactggtgtg gccgaggacc acgatgaccc gcgagcccgc
     2701 caccttggcg gcgtactcga ggctgcccag gatgtcctca ttgactgtgt tgccggccac
     2761 gcgggcgacg aagaggtcgc cgatgctctg gtcgaagacg atctccggtg cggcgcggga
     2821 gtcgatgcag cccaccacgc tcgcgaaggg gaactgcccg agcgcggtgg tctgcacgcg
     2881 ggccttgagg ttgcgggcaa ggggcttgcc ggc
//
LOCUS       DAOQPA010000229         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_26393_length_2904_cov_20.358722, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000229 DAOQPA010000000
VERSION     DAOQPA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26393_length_2904_cov_20.358722"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKX01_17220"
     CDS             complement(<1..742)
                     /locus_tag="PKX01_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77929.1"
                     /translation="MSDTLRRRPMLPTRTGTLRLKSGTPTPAGDRPATQDWRPPAARR
                     GKASMPEQANTESNRRPPREGDVRGDVPPRRFGDERPPRTDECRPGRNADERFRRDDN
                     GHPPRADFRKRRDDTDRDPRRFDDDRRPARSDDTGVRRDRDTRPARPFRLPDERRREA
                     EDGRPPRGGFRDRRDDAPDRAPRRFEDERPPRNDDGRPGRAADERFRHDDDRRPPRGD
                     FRERPRREDIDRGPRRFADERPARGPQEA"
     gene            complement(753..1469)
                     /locus_tag="PKX01_17225"
     CDS             complement(753..1469)
                     /locus_tag="PKX01_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ77930.1"
                     /translation="MKLDRLLQAQGFGSRRQCRQLVELGEVRVRGDVVDDPDAAIDTA
                     GLTYEVAGEAWIYREKTYLALFKPAGFECSHEPKHHRSVYSLLPHPLVVRNVQSVGRL
                     DVDTTGLLLFSDDGAFIHTLSSPKKHVPKIYRAELAEPAADTLVPRLLAGVDLIDEPR
                     PVAALDACLRGPTTLEMRLDLGKYHVVKRMIAAAGNHVQTLHRSGFGGLSLGEGPLAG
                     LAEGQFIYLDADALRQLRAV"
     gene            1752..2900
                     /locus_tag="PKX01_17230"
     CDS             1752..2900
                     /locus_tag="PKX01_17230"
                     /inference="COORDINATES: protein motif:HMM:NF018809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalM family protein"
                     /protein_id="HMZ77931.1"
                     /translation="MLNNFGRSSRWFCFALVWSVLLSGCANVAFDHGVSRLDGKALVF
                     GRIVLDREGERLVISPFSMPVVIRDISSADEPRMLAQKFENDGRFYWALPPGRYQVSI
                     VLHNYAGGVVSYSFALERSGRSYYFGDLILHGKKRFDSVGSANMRDIKPEFVNDLQGA
                     KAELLRRNPQLDAASIDRLTVRDMTSPEQRGVAYGEVLSELRPCCASLASLPYKKLSS
                     GQRGEDTISSNSPVFDFPEGRSRFVAWELSAPSMQNTVALRSVVTPSGLPGTGQFYIF
                     SPAVMLLDENFNVLADLQRGLFVPVPASMMPPRSASLQARIPLNAQTARVRYLVVYTT
                     RSIIEGAWSTSRPGFVPIAGGVLPTGIPIGVVMEPAISGIIEAELSRH"
BASE COUNT          451 a          827 c         1060 g          566 t
ORIGIN      
        1 gcgcctcctg agggccccgg gcggggcgtt catcggcgaa gcggcgtggc ccgcggtcga
       61 tgtcctcgcg gcggggacgc tcgcggaaat cgccacgggg cggacggcgg tcgtcgtcgt
      121 gtctgaagcg ctcgtcggcc gcgcgcccag ggcgaccgtc atcgttccgc gggggacgtt
      181 cgtcctcgaa gcggcgcggg gcgcggtccg gggcgtcgtc ccggcggtcg cggaatccgc
      241 cacggggcgg acggccatct tcggcttcgc ggcggcgttc gtccggcagg cggaaggggc
      301 gggccggacg ggtgtcgcgg tcacgacgga cgcccgtgtc gtcgcttcgg gcaggacggc
      361 ggtcgtcgtc gaagcggcgc gggtcgcggt ccgtgtcgtc ccgacgtttg cggaaatcgg
      421 cacggggcgg gtggccgttg tcgtctcggc ggaagcgttc gtcggcgttg cggccagggc
      481 ggcactcgtc tgtgcgaggg gggcgctcgt cgccaaagcg gcgcggcggc acgtcgccgc
      541 ggacgtcccc ttcgcggggc gggcggcggt tggattcagt attggcctgc tcgggcatgg
      601 acgctttgcc gcggcgggcg gcgggcgggc gccagtcctg ggtcgcgggg cggtcgcccg
      661 ccggggtagg tgtgccggac ttgaggcgca gggtgcccgt gcgcgtgggc agcatgggac
      721 ggcggcggag ggtgtcggac atggcgtccc tttcagacgg cgcggagctg acgcagggcg
      781 tcggcgtcca ggtagatgaa ctgcccttcg gcaagtccgg cgagggggcc ttcacccagg
      841 ctgaggccgc cgaaaccgct gcggtggagg gtctgtacgt ggttgccggc cgccgcgatc
      901 atgcgcttga ccacgtggta cttgcccaga tcgaggcgca tttcgagggt cgtggggccg
      961 cgcaggcagg cgtcaagcgc ggcgaccggg cggggctcgt cgatcaggtc gacgccggcc
     1021 agcagacgcg ggacgagggt gtcggcggcg ggctcggcga gttcggcccg gtagatcttg
     1081 ggcacgtgct tcttggggga ggacagggtg tggatgaagg cgccgtcgtc ggagaagagg
     1141 agcagaccgg tggtgtccac gtccagccgg ccgacgctct gcacgttgcg caccaccagc
     1201 gggtggggca gcaggctgta cacgctgcgg tggtgcttgg gctcgtggga gcactcgaag
     1261 ccggcgggct tgaacagcgc gaggtaggtc ttttcccggt agatccaggc ctcgccggcg
     1321 acttcatagg tgagaccggc tgtgtcgatg gcggcgtccg ggtcgtccac gacatcgcca
     1381 cgcacgcgca cctcgccgag ttcgacgagt tggcggcatt ggcggcggct gccgaagccc
     1441 tgagcctgga ggagacggtc aagtttcacg gtgggaggcg agggccggaa ggcctcgtgg
     1501 ggcgcagcgg atgcaaaaat tggcgggaag gaggccagaa ggggcgtatt ttggcagaaa
     1561 acgtggcgaa gggcactgca tccgctccgg tactgttctg ccacctgccc gcagacgtgg
     1621 cgcgtgcacg caggtgcctc tcaagtgcga cgatcccggg caagaccgtc ggagcgcaaa
     1681 gccaattcga gcggagatcg ttgcacgtat ggctttattg tatgggcgga tatttcgtgg
     1741 gagtgtcggc aatgctcaat aattttggaa ggtcatcccg gtggttctgt tttgcccttg
     1801 tctggagcgt gttgctgagt ggctgtgcca atgtcgcatt tgatcatgga gtgtccaggc
     1861 tggacggaaa ggccctggtc tttggccgga tcgtccttga tcgagagggc gaaaggcttg
     1921 tcatttcacc gttctccatg cccgtcgtca ttcgcgacat atcgtcagcc gacgaaccgc
     1981 gcatgctggc gcagaaattc gagaatgatg gacgctttta ctgggcgttg ccgcccgggc
     2041 gatatcaagt cagcatcgtg ctccacaact acgccggagg tgtcgtttcc tatagctttg
     2101 ctctcgagcg ctccggaaga tcctattact tcggtgatct gattctgcac gggaagaagc
     2161 gttttgacag cgtcggcagc gccaatatgc gcgatataaa gccggaattt gtcaatgatc
     2221 ttcagggcgc caaagccgaa ctcctccgcc gaaatcctca gctggatgcg gcgtccattg
     2281 accgcctgac agtcagggat atgacttctc ctgagcagcg cggcgtcgct tatggcgaag
     2341 tcctgagtga attgcgtccg tgctgcgcca gcctcgcgag cttgccctat aaaaaattga
     2401 gctccggtca gcgtggtgag gatacgatca gttcaaatag tccagtgttt gactttcctg
     2461 aagggcgaag ccgattcgtt gcgtgggagc tgtccgcgcc atcgatgcag aacaccgtgg
     2521 ctttgcgaag cgtggtgacg ccgagtggat tgcccggcac tgggcagttt tatatttttt
     2581 cgccagcggt catgctgctc gacgagaatt tcaacgtcct cgccgatctt cagcgcggac
     2641 tcttcgtccc ggtcccggca tcgatgatgc ctccgcgttc agcctcgctc caggcgcgca
     2701 ttcccctgaa cgcgcaaacg gccagggtac gataccttgt tgtctataca actcggtcga
     2761 tcattgaagg ggcgtggagt acttcgcgcc cgggatttgt tccaattgcg ggtggcgttt
     2821 tgcccacggg gattcccatc ggcgtcgtca tggaacctgc tatatctgga atcatcgagg
     2881 cggagctatc acggcattga tacg
//
LOCUS       DAOQPA010000230         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_27602_length_2832_cov_17.233705, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000230 DAOQPA010000000
VERSION     DAOQPA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27602_length_2832_cov_17.233705"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKX01_17235"
     CDS             <1..182
                     /locus_tag="PKX01_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ77932.1"
                     /translation="QFVLAGNIIVRQRGTEYHPGENVGIGKDHTLFALTEGTVQFTVK
                     GANKRRTVSIVAAAE"
     gene            360..1499
                     /gene="obgE"
                     /locus_tag="PKX01_17240"
     CDS             360..1499
                     /gene="obgE"
                     /locus_tag="PKX01_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ77933.1"
                     /translation="MKFFDEARIDVIAGDGGNGAATFRREKYIPKGGPDGGDGGKGGD
                     VYAIADRNLNTLIDYRYKRTFTAERGENGRGADCYGKGGEDIELRMPVGTVISEQESG
                     EVIADLDHDGKRVMIARGGRGGLGNLHFKTSTNRAPRKKTMGEAGERKSLNLELKVLA
                     DVGLLGMPNAGKSTFIRAVSSARPKVADYPFTTLAPNLGVVRTSENRSFVIADIPGLI
                     EGAADGAGLGHQFLRHLQRTHILLHIVDIAPFDPEADPVRDAHAIVEELYKYDESLAA
                     KRRWLVLNKLDLIPDAEERAARVQDFLAAYGPVERHFQVAAISGERCQEVVFAIQDLL
                     DAEKAAAQAAADEAARAAAAHAADADAALDDTDIDHPDSDESSPA"
     gene            1496..2614
                     /gene="proB"
                     /locus_tag="PKX01_17245"
     CDS             1496..2614
                     /gene="proB"
                     /locus_tag="PKX01_17245"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HMZ77934.1"
                     /translation="MRTRIQNARRLVVKVGSALVTNNGAGLAVEALAEWARQIARLRA
                     DGREVVLVSSGAIAAGMQRLGWSKRPQEMHHLQAAAAVGQMGLAQAYESVFARNDLHT
                     AQILLTHADLADRTRYLNARSTLVTLLELGVIPIINENDTVVTDEIKFGDNDTLGALV
                     ANLIEADALIILTDQQGLYTADPRNDPSATLISEERAENTALEAMAGGAGSGISKGGM
                     ITKVRAAQRAARSGAHTCIASGREPDALLRLVGGDDLGTLLYASSTPLAARKQWLADH
                     LQLAGSLILDDGAVDALRAGRSLLPVGVTAVEGDFNRGAVVACRTASGIELARGLINY
                     ASSDARRIARSPSSEIEHLLGFAEAPELIHRDNLVLIG"
BASE COUNT          532 a         1063 c          864 g          373 t
ORIGIN      
        1 gccagttcgt cctcgccggc aacatcatcg ttcgccagcg cggcaccgaa taccatccgg
       61 gcgaaaacgt cggcatcggc aaggaccaca ccctgttcgc gctgaccgaa ggcaccgtgc
      121 agttcaccgt caagggcgcc aacaagcgcc gcaccgtgag catcgtcgct gccgccgaat
      181 aagcgcagca cacggtttgc caaagcccta tcgtgcgata gggctttttt cattcagggg
      241 acgcacgttc gcggcgccga ccacaggccc caccgcagcg ctgaccgagc accggatccg
      301 catgccgctt cgccggcaag gccccaccgc tcgtcgccca tcacccaccg gatacgccca
      361 tgaaattctt cgacgaagcc cgtatcgacg tcatcgccgg cgacggaggc aatggcgcgg
      421 ccaccttccg tcgcgaaaag tacattccca agggcggacc ggacggcggt gacggcggca
      481 agggcggcga cgtttacgcc atcgccgacc gcaacctgaa caccctcatc gactaccgct
      541 acaagcgcac cttcaccgcc gagcgcggcg agaacggacg cggcgccgac tgctacggca
      601 agggcggcga ggacatcgag ctgcgcatgc cggtgggcac ggtcatttcg gaacaggaga
      661 gcggcgaagt catcgccgac ctggaccacg acggcaagcg cgtcatgatc gcccgcggcg
      721 ggcgcggcgg cctcggcaac ctgcacttca agaccagcac caaccgcgcc ccgcgcaaga
      781 agaccatggg cgaggccggc gagcgcaaga gcctcaacct ggagctcaag gtgctcgccg
      841 acgtgggcct gctgggcatg cccaacgcgg gcaaatccac cttcatccgc gccgtgtcct
      901 cggcccgccc caaggtggcc gattatcctt tcacgaccct cgccccgaac ctgggcgtgg
      961 tgcgcacctc agagaaccgc agcttcgtca tcgccgacat tcccgggctg atcgaaggcg
     1021 ccgccgacgg cgccggcctc ggtcaccagt tcctgcgcca cctgcagcgc acccacatcc
     1081 tgctccacat cgtggacatc gcccccttcg atcccgaagc ggacccggtg cgtgacgctc
     1141 acgccatcgt cgaggagctg tacaagtacg acgagagcct cgccgccaag cgtcgctggc
     1201 tggtcctcaa caagctcgac ctgattcccg atgcagagga acgcgccgcc cgcgtgcagg
     1261 acttcctcgc cgcctacggg ccggtcgagc gccacttcca ggtcgcggcc atcagtggcg
     1321 agcgctgcca ggaagtggtc ttcgcgatcc aggacctgct cgacgccgaa aaagccgccg
     1381 cccaggctgc cgccgacgag gccgcccgcg cggcagcggc ccatgccgcg gacgcggatg
     1441 ccgcgcttga cgacacagac atcgaccacc ccgacagcga cgaaagcagc cccgcatgag
     1501 aacccgcatc cagaacgccc gccgcctggt cgtcaaggtc ggcagcgccc tcgtcaccaa
     1561 caacggcgcc ggcctcgccg tcgaagcgct ggccgaatgg gcccgccaga tcgcccgcct
     1621 gcgggccgac ggccgcgaag tggtactggt gtcgtccggc gccatcgccg ccggcatgca
     1681 acgcctcggc tggagcaagc gcccccagga aatgcaccac ctgcaggccg ccgccgcggt
     1741 cggccagatg ggcctcgccc aggcctatga atccgtgttc gcccggaacg acctgcacac
     1801 cgcccagatc ctgctcaccc atgccgacct ggcggaccgc acccgctacc tcaacgcccg
     1861 ctcgacgctg gtgaccctgc tggagctggg cgtcattccc atcatcaacg agaacgacac
     1921 cgtcgtcacc gacgagatca agttcggcga caacgatact ctcggcgccc tcgtcgccaa
     1981 cctgatcgaa gccgacgccc tgatcatcct caccgaccag cagggcctgt acaccgccga
     2041 tccgcgcaac gatccgagtg ccaccctgat ctccgaggag cgcgccgaga acaccgccct
     2101 cgaagccatg gccggcggcg ccggcagtgg catcagcaag ggcggcatga tcaccaaggt
     2161 ccgcgccgcc caacgcgccg cccgcagcgg cgcccacacc tgcatcgcca gcggccgcga
     2221 gcccgacgcg ctgctccgcc ttgtcggtgg agatgacctc ggcaccctgc tgtacgccag
     2281 cagcacaccg ctggcggccc gcaagcagtg gctcgcagat cacctgcagc tggccggcag
     2341 cctgatcctc gacgacggcg cggtggacgc cctgcgggcc gggcgcagcc tgctgccggt
     2401 aggcgtcacc gccgtggaag gcgacttcaa ccggggggcc gtggtggcct gccgcaccgc
     2461 gagcggcatc gaacttgcgc gcgggctgat caactacgcc agctccgacg cacgccgcat
     2521 cgcccgcagt ccatccagcg aaatcgagca cctgctgggc tttgccgaag cgccggaact
     2581 gatccaccgg gacaatctgg tgctgatcgg ctgaaggcgg acggacccag taaaaaaggc
     2641 gacctgaggt cgcctttttt acatgtttcg gtgcgccgtg cgttccggtg ccgaaccacc
     2701 tccagccatg cgcggggccg tcgtccacga aaaaacgggt gcccgaaggc acccgtttga
     2761 tttccaaatc cgaacgagcc ggaattagaa ggtgtgacgc agaccgacgg acacgccgga
     2821 cacggtggag cc
//
LOCUS       DAOQPA010000231         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_29202_length_2745_cov_20.992193, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000231 DAOQPA010000000
VERSION     DAOQPA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29202_length_2745_cov_20.992193"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="PKX01_17250"
     CDS             complement(<1..1573)
                     /locus_tag="PKX01_17250"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HMZ77935.1"
                     /translation="MTREAMEYDVVIVGAGPSGLTTAIRLKQLAEQNGKELSVCVVEK
                     GSEVGAHILSGAVIEPRALEELFPDWKERGAPLNTPVVEDRFLFLTTEKAYKLPTPPQ
                     MHNHGNYIVSLGNVVRWLGQQAEELGVEIYPGFAASEVLYHDDGELRGSVKGIATGDM
                     GVEKNGEPGPNYQPGIELHARQTVFSEGCRGSLTKGLFEKFHLRDGADPQTYGIGIKE
                     LWEVRPEVHQPGLTVHTVGWPVGTDVYGGSFLYHLENNQVAVGFVVGLDYSNPHLSPY
                     EEFQRFKTHPEIRTFFEGGRRISYGARALNEGGFQSVPRLSFPGGVLVGDTAGFLNVP
                     KVKGTHMSMKSGIEAAEALFAHLTAEGQHGHECIGYPERLKQSWLWDELYTVRNIRPS
                     FRWGLWGGIAYSALDTFVLKGRAPWTLHHHADHTQLRKASECAPIVYPKPDGKISFDR
                     LSSVFISATNHTEDQPCHLRLKDASVPIATNLALYDAPETRYCPAGVYEIVTEDSGPR
                     MQINAQNCLHCKTCDI"
     gene            complement(1724..>2745)
                     /locus_tag="PKX01_17255"
     CDS             complement(1724..>2745)
                     /locus_tag="PKX01_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ77936.1"
                     /translation="GIHGSATAVMSFGDKGGAIGYLVGEANRGLEYMFTMMNHARLNV
                     GLEGVGVSERSYQHALAYARERIQGKIVGDKSGEKKPILHHPDVRRMLMDMKSRTEAG
                     RALAYYVAGCMDRAHSHPDADVRAANQRRLELLTPVVKGWCTENAQGITWNGVQVHGG
                     MGFIEETGAAQYMRDARIITIYEGTTAIQANDLIGRKTAKEGGKSMQQLLADIAETGA
                     ALRASGNDQLKTIAEALGDGIAALDEATQWLLANYDATPQAAHAGSVPFLKLTGIVVG
                     AWLLARSAAIAAERLDQADAAYYTAKLATATYFAQHVVPEANSYRDAIVNGAAAVLAL
                     DEALF"
BASE COUNT          368 a          810 c         1060 g          507 t
ORIGIN      
        1 tgatgtcgca ggtcttgcag tgcaggcagt tctgggcgtt gatctgcatg cgcggaccgc
       61 tgtcttcggt gacgatctcg tacacgccgg ccgggcagta gcgggtctcc ggcgcgtcgt
      121 agagggccag gttggtggcg atgggcaccg acgcgtcctt caggcgcagg tggcagggct
      181 ggtcctcggt gtggttggtg gccgagatga agaccgagga caggcggtcg aagctgatct
      241 tgccgtcggg cttgggatag acgatgggtg cgcactccga cgccttcctg agctgggtgt
      301 ggtcggcgtg atggtgcagg gtccacggtg cgcggccctt gagcacgaag gtgtcgaggg
      361 cgctgtaggc gatgccgccc cacaggcccc agcggaagct cgggcggatg ttgcgcacgg
      421 tgtacagctc gtcccacagc cagctctgct tgaggcgctc cggatagccg atgcactcgt
      481 ggccgtgctg gccttcggcg gtgaggtggg cgaagagggc ttcggcggcc tcgatgccgg
      541 acttcatgga catgtgggtg cccttgacct tgggcacgtt gaggaagccg gcggtgtcgc
      601 ccaccagcac gccgccgggg aagctcagcc tgggcacgga ctggaagccg ccttcattga
      661 gggcgcgggc gccgtagctg atgcgccggc ccccttcgaa aaacgtgcgg atctccgggt
      721 gggtcttgaa gcgctggaat tcctcgtagg gggacaggtg cgggttgctg taatccagac
      781 ccaccacgaa gcccacggcg acctggttgt tttccaggtg gtagaggaag gagccgccat
      841 atacgtcggt accgacgggc cagcccacgg tgtgcacggt gaggccgggc tggtgcacct
      901 cggggcgcac ttcccatagt tccttgatgc cgatgccgta ggtctggggg tcggcgccgt
      961 cgcgcaggtg gaacttctcg aacaagccct tggtgaggga gccgcggcag ccctcggaga
     1021 agacggtctg gcgggcgtgc agctcgatgc ccggctggta gttggggccg ggttcgccgt
     1081 tcttctcgac gcccatgtca ccggtggcga tgcccttgac cgagccgcgc agctcgccgt
     1141 cgtcgtggta gagcacttcg gaggcggcga agccggggta gatctcgacg cccagttcct
     1201 ccgcctgctg gccgagccag cgcaccacgt tgcccaggct cacgatgtag ttgccgtggt
     1261 tgtgcatctg gggcggggtg gggagcttgt aggccttttc ggtggtcagg aacaggaagc
     1321 ggtcctcgac gaccggggtg ttgaggggcg cgccgcgttc cttccagtcg ggaaacagtt
     1381 cttccagagc gcggggttcg atgacggcac cggagaggat gtgggcgccg acttcggagc
     1441 ccttttcgac gacgcacacc gagagctcct tgccgttttg ctcggccagt tgcttcaggc
     1501 ggatcgcggt ggtcaggccg gaggggccgg caccgacgat caccacgtcg tattccatcg
     1561 cttcgcgcgt catctgcttc tccgaaaatc gagcgtgttc agttgaatct gggctgcatc
     1621 cgggcacgcg gtgcccgggt gggggaaagg ggccgccggg gatcgcctcc cgcggggccg
     1681 ccacaggggc ggcaccgcgg gcggggcccg gcgggggaga cggtcagaac agggcttcgt
     1741 ccagggccag caccgcggcg gcgccgttca cgatggcgtc ccggtagctg ttggcttcgg
     1801 ggaccacgtg ctgggcgaag taggtggcgg tggccagctt ggcggtgtag taggcggcgt
     1861 cggcctggtc caggcgttcg gcggcgatag cggcggagcg ggccagcagc caggcgccga
     1921 caacgatgcc ggtgagcttg aggaagggca ccgagccggc gtgggcggcc tggggcgtgg
     1981 cgtcgtagtt ggcgagcagc cattgggtgg cttcgtccag cgcggcgatg ccgtcgccca
     2041 gggcttcggc gatggtcttg agctggtcgt tgccggaggc ccgcagggcg gcgccggtct
     2101 cggcgatgtc ggcgaggagc tgctgcatgc tcttgccgcc ttccttggcg gtcttgcggc
     2161 cgatcaggtc gttggcctgg atggcggtgg tgccttcgta gatggtgatg atgcgggcgt
     2221 cgcgcatgta ctgggctgcg ccggtctctt cgatgaagcc catgccgccg tgcacctgca
     2281 cgccgttcca ggtgatgccc tgggcgttct cggtgcacca gcccttgacc accggggtga
     2341 gcagttcgag gcggcgctgg ttggcggcgc gcacgtcggc gtccgggtgg ctgtgggcgc
     2401 ggtccatgca gccggccacg tagtaggcca gggcgcggcc ggcttcggtg cgggacttca
     2461 tgtccatcag catgcggcgc acgtccgggt ggtgcaggat gggcttcttc tcgccggact
     2521 tgtcgccgac gatcttgccc tggatgcgtt cgcgggcgta ggcgagggcg tgctggtagc
     2581 tgcgctcgga gacgccgacg ccttccaggc ccacgttgag gcgggcgtgg ttcatcatcg
     2641 tgaacatgta ttcgaggccg cggttcgcct cgcccaccag gtagccgatg gcgccgccct
     2701 tgtcgccgaa ggacatgacg gcggtggcgc tgccgtggat gccca
//
LOCUS       DAOQPA010000232         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_29271_length_2742_cov_13.008188, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000232 DAOQPA010000000
VERSION     DAOQPA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29271_length_2742_cov_13.008188"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1099)
                     /locus_tag="PKX01_17260"
     CDS             complement(146..1099)
                     /locus_tag="PKX01_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77937.1"
                     /translation="MTSPIRPLRVPPTPCAPRVMRASDHPLGIDRAIAATLSAVLRPA
                     WQAWLQAPRVRLLERLGPLGRGVIAIAPERSAEDGCSTDLDPALTGRWLTLRVLDERG
                     RVCELSAPDATLAQLADDLDRLDDTRLAHALADCLRQDVLQAVCSDEESPWPVLRHAI
                     PHANAAAELLFLAPEGRFDPGVRGALLPALRQRERQLQEAAHQHLAQGLDRSLAARLH
                     SVGVAPTLTHINWLAGTASDVQARRLHALLIAPRFCRAFLTGDVAARGAFEAAVDAGA
                     SLWSFLEAHYRLPVVGLRDADPPDRSGSHAPRNGPPLRRQG"
     gene            1334..2536
                     /gene="csm6"
                     /locus_tag="PKX01_17265"
     CDS             1334..2536
                     /gene="csm6"
                     /locus_tag="PKX01_17265"
                     /inference="COORDINATES: protein motif:HMM:NF021162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HMZ77938.1"
                     /translation="MTNDWSGRAGEGAPEGDGEWALSMPVRDRPPESFARRVLVAVGG
                     LAPQVITEALYWLACVRVPPFVPTEIRVVTTTVGREQCELALLHPQQGKFHALLRDYG
                     LVGQVAFGSEHFELIRDGLGRALDDVCDGPENVAAADSITAFIRDLAADPDCALHVCI
                     SGGRNTMGVYLGYALSLFGRRQDRLSHVMVPPEFTHNHDFYYPPPQPGVLIDRISNRS
                     VSTRAASVTLAEIPFVSLRGLLPSEVLDGVVSYSTAVALTRESMQPHRLVVARASRRL
                     LCGEREVALPPQLFAWYLWLAQRRKSGAEHGGAVRWTDADIAGTFLACYRSVVGIASH
                     DYDTAAEVLRDGMTDTFFAEKKSRVNRALRTALGVDAEAYLIHETGRRPATRFGLLLA
                     PQAIEFRD"
BASE COUNT          390 a          904 c         1022 g          426 t
ORIGIN      
        1 gtgggcatgc agggcatgtc cgatggtatc cacgatgaac gagtcggtga ccatgagggt
       61 tccccggaac ggaagggcaa tgcatcgttg gcgtgcatga tacgcctcct ggcgagggcc
      121 ggcgcggcat gccggccgga cgaggtcagc cctgccggcg gagcggcggg ccgttgcggg
      181 gcgcgtgact gcccgagcgg tccggagggt cggcgtcccg caggcccacg accggcaggc
      241 ggtaatgggc ttctaggaag gaccacagcg acgcgcccgc atcgaccgcc gcctcgaagg
      301 ctccccgcgc cgccacatcg ccggtcagga atgcccggca gaagcggggg gcgatgagca
      361 gtgcgtggag gcgacgcgcc tgcacgtccg acgccgttcc tgccagccag ttgatgtggg
      421 tgagggtggg cgccacgccg acggagtgca ggcgggccgc cagggatctg tccaggccct
      481 gcgccaggtg ctggtgggcc gcctcctgca gttgccgctc gcgctggcgc agggcgggca
      541 gcagcgctcc ccgcacgccc gggtcgaagc gtccttccgg cgcgagaaag agcaactccg
      601 cagcggcgtt ggcgtgggga atggcgtggc ggaggacggg ccacggggat tcttcgtcgc
      661 tgcagaccgc ctgcaggaca tcttggcgca agcagtcggc gagtgcgtga gcgaggcgcg
      721 tgtcgtcgag gcggtcgaga tcgtcggcga gctgcgccag cgtcgcgtcg ggggcgctca
      781 gttcgcacac gcgaccacgc tcgtcgagca cccgcagggt cagccaccgg cccgtcagtg
      841 cggggtcgag atcggtgctg cagccgtctt ccgcgctgcg ctccggggcg atggcgatca
      901 cgccgcgtcc gagcgggccg aggcgttcga gcaggcggac gcggggggcc tgcagccatg
      961 cctgccaggc cggacgcagg actgccgaaa gggtcgccgc gatggcgcgg tcgatgccga
     1021 gcgggtggtc gctggcgcgc atgacgcgcg gggcgcaggg ggtgggcggg acgcggaggg
     1081 ggcggatcgg tgacgtcatc gtagtgagct cccgtgaaat gcggtctggt gccgcgatga
     1141 gcccagctta atgcgcgccc cccgcgatgc gccgacatga caacgttgcc gccagggtca
     1201 tcgcttcagg ggcgcgggag cccgctgcgc cgaccgattc ccgcgccggc tgcaggcttt
     1261 ttcaagacaa gcttgccatg gtgaatatct gtcacgcgta tgcgctacag tgcgaggctt
     1321 ggggagaaaa gcgatgacga acgactggag tggccgggcg ggcgaaggtg caccggaagg
     1381 cgacggggag tgggccttgt cgatgccggt cagggaccgg ccgcccgagt cctttgcccg
     1441 ccgtgtgctg gtggcagtgg gcgggctggc gccgcaggtg atcacggagg ccctgtactg
     1501 gctggcctgt gtgcgggtgc cgcctttcgt acccaccgag atccgcgtcg tcaccaccac
     1561 cgtcgggcgc gagcaatgcg agctggcgct gcttcatccc cagcagggca aattccacgc
     1621 cctactgcgg gattacggcc tggtcggcca ggtggccttc ggcagtgaac acttcgagct
     1681 gatccgcgac ggcctcgggc gggcgctgga cgacgtctgc gatgggcccg agaacgtggc
     1741 cgcggcggac tccatcaccg ccttcatccg cgatctggcg gccgatccgg attgcgcgct
     1801 gcacgtgtgc atctcgggcg ggcgcaacac catgggcgtc tatctcggct atgccctgag
     1861 cctgttcgga cggcgccagg atcgtctgtc ccacgtgatg gtgccgccgg aattcaccca
     1921 caaccacgat ttttactacc cgccgcccca gcccggggtg ctcatcgacc gcatcagcaa
     1981 ccgttccgtc agcacccgtg cggcgtcggt caccttggcg gagatcccct tcgtcagcct
     2041 gcgcggcctg ctgccgagcg aggtgctgga cggcgtggtg agctattcca ccgcggtcgc
     2101 cctgacccgt gaatccatgc agccccaccg gctggtggtg gcccgggcga gccggcggct
     2161 gttgtgcggc gagcgggagg tggccctgcc gccccagctg ttcgcctggt atctgtggct
     2221 ggcccagcgc cgcaagagcg gggcggaaca tggcggtgca gtgcgctgga ctgatgcgga
     2281 catcgccggc accttcctgg cctgctaccg gagcgtggtg gggatcgcct cccacgacta
     2341 cgacacggcg gcggaggttc tgcgggacgg catgaccgac acctttttcg ccgagaagaa
     2401 gtctcgcgtc aatcgggctc tgcgtacggc cctgggcgtg gatgcggagg cttacctgat
     2461 ccacgagact ggccgccgtc cggccacccg cttcggcctg ctgctggcgc cgcaggcgat
     2521 tgagttccgc gactgaggcc tgcgtgccgc ggagccgagg cgtcctgaaa agtcggacgg
     2581 ggcccgcggg ccccgtccat cgggtgatgc cgatgcgcgg acgcgctcac accttccgat
     2641 acacctcggc gccggtgcgc acgaactcga cggccttcgt ttccatgccc ttctccagcg
     2701 cctcggcctc gccgatgccc tgctgggcgg cgaagtcgcg ca
//
LOCUS       DAOQPA010000233         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_30143_length_2698_cov_16.614075, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000233 DAOQPA010000000
VERSION     DAOQPA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30143_length_2698_cov_16.614075"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(874..1692)
                     /locus_tag="PKX01_17270"
     CDS             complement(874..1692)
                     /locus_tag="PKX01_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77939.1"
                     /translation="MGLREHLVAGTPCMLDFAGFDRPLGVLVGGALQRLAPWTYRDHL
                     DALRACTRLVGPALRLDAAAFADRVLAPLDLPAEERPSLHPIALWWAAGGDDPLLPPP
                     AEGDWLDLGSARARLRPWTEHQRLATLDGCLDADQDTPDATDSFDPVGYLDGMVRASL
                     AVLEGPVPLERLDARASAVLLNATVALNVVDEADDTLLQGGPAARLAATRTLRLCRAL
                     GWTPTRVWSTPAAEVDRLLRLLDLLEPPARPVPAHRGLADHPDATVIRFEDDAA"
     gene            complement(1697..2089)
                     /locus_tag="PKX01_17275"
     CDS             complement(1697..2089)
                     /locus_tag="PKX01_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77940.1"
                     /translation="MPTVFSANRSSVLVDGEAIEGLQSLAYRVVTEREDIRAVGSDER
                     VAVSFGLRTVQGELTVRSASYKLDGHLDKQAKFQLVANLKKDDAADSPKRTVSFDDCY
                     VEDKSFSLGAGGTVVTAYTFSATRVREE"
     gene            complement(2151..>2698)
                     /locus_tag="PKX01_17280"
     CDS             complement(2151..>2698)
                     /locus_tag="PKX01_17280"
                     /inference="COORDINATES: protein motif:HMM:NF037565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HMZ77941.1"
                     /translation="SDRFILVAPAGVVGAVAGMTGSLDYFQSPTFKRLSGLGEPAPAL
                     GVESQQELLRKNVVPIVAQRGRGIIVVRGLTTDGDQISVRRVADHAVRGVKTIGELFI
                     GRLNNEEGRGALKQKLVEFLVQMQKEGAIVPSTDGTDPAFKVNVYSSQQDFAQGIVRV
                     DLAVRPVRAIDYIYATVLVQV"
BASE COUNT          364 a          816 c         1141 g          377 t
ORIGIN      
        1 cggctgggac cgcgccccgc gacgcaggag gcgcagaagg ggccgtgggc gcggcagagg
       61 gcgaaggtgt ggcgacccca tcgtggcggg gcacggcggg gcgtacgggg agacggaggg
      121 caggtgtgcc ggttgcggct gggaccgcgc cccgcgacgc aggaggcgca gaaggggccg
      181 tgggcgcggc gggggcggca tcgccggggc gtgtcacgtc aggcgcttgc gctccgatga
      241 tcggggctga catccccggg ctgcgtgcat caacgccgtg cgcgtcctga acgcgtgccc
      301 tgacgagggg gacgcggagg gagacctggg gagcgggttc agtgggcagc ggatcgccgg
      361 gcacctgtgt gtgcatcccc tcgggggggc ggtgggtgac atccggtgct gtgtgcgaca
      421 ttcgttcggc caccggtatc ggaggagggg gggcgcgccg atcgcccagg cccgcctcgg
      481 gcgcgtgggt ggggacgcgc tgggctgtgc tcgccggggt gggccgactg tgcggggccg
      541 ctgccgcccg atccggcggg gtgggcgccg tggccagggc ccgcggagcg gatgcgggcg
      601 gccaggcgtc ggtcgttggt tgttccgggg gtcgggcaat gacggcaggg gcggtgggac
      661 gcgagggcgc gaccgtctcg atggcgggcg tcagcggggc gacacgggcg ggaaaagcgg
      721 gccgtgctgg cagtccgtcc gtgggctccg ggcgggcgtc gtcagtcgcg tcggcatccg
      781 ggtccgccag gtcgaggtgg cggcgcgcgc catcgagact gcgggcaagg ggcgcgagca
      841 ggcgggcgac gaggcgctcc gtgtgggcgg tgctcatgcg gcgtcgtcct cgaaacggat
      901 cacggtggcg tccgggtgat cggccaggcc acggtgggcg ggcaccgggc gggccggtgg
      961 ctccagcagg tcgagcaggc gcagcaggcg gtccacttca gccgcggggg tgctccagac
     1021 gcgcgtgggc gtccagccga gggcgcggca caggcgcagg gtgcgggtgg cggccagacg
     1081 ggcggccggg ccaccctgca gcagggtgtc atcggcctcg tccacgacat tgagggcgac
     1141 cgtggcgttg agcaggacgg cgctggcgcg ggcgtcgagg cgttccagtg ggacggggcc
     1201 ctcgaggaca gcgaggctgg cgcggaccat gccgtccagg tagccgacgg ggtcgaagct
     1261 gtcggttgcg tccggggtgt cttggtcggc atcgaggcag ccatcgaggg tggcgaggcg
     1321 ctggtgttcg gtccacgggc gcagccgggc gcgggcgctg cccaggtcga gccagtcgcc
     1381 ctcggcgggc ggcggcaaca gggggtcgtc tccgcccgcc gcccaccaca gggcgatggg
     1441 atgcagcgac ggacgctctt cggcgggcag gtcgaggggc gcgaggaccc ggtcggcaaa
     1501 ggcggcggca tccaggcgca gggctgggcc gaccagccgg gtgcaggcgc gcagggcgtc
     1561 caggtgatcc cggtaggtcc acggcgccag gcgctggagt gcgccgccca cgagcacgcc
     1621 aaggggccgg tcgaagcccg cgaaatccag catgcagggg gtgccggcga cgagatgctc
     1681 gcgcaggccc aagggtctac tcctcgcgga cgcgggtcgc gctgaaggtg taggcggtca
     1741 ccacggtgcc accggcgccc aggctgaagg acttgtcctc cacgtagcag tcgtcgaagg
     1801 agacggtgcg cttgggtgaa tcggcggcat cgtccttctt gaggttggcg acgagctgga
     1861 acttggcctg tttgtccaga tggccgtcga gcttgtagct ggcggaccgt acggtgagct
     1921 cgccctgcac ggtgcgcagg ccgaagctca cggcgacgcg ctcgtcgctg cccacggcac
     1981 ggatgtcctc ccgttcggtg acgacccggt aggccaggga ttgcaggcct tcgatggctt
     2041 ccccatcgac caggacgctg gagcggttgg cggaaaagac ggtcggcatg gcgggactct
     2101 ccggagatgt cgagggtgcg ggatcttatg tcgcggggtg ctgccgggcg tcagacctgg
     2161 accagcacgg tggcgtagat gtagtcgatg gcgcgcaccg ggcgcacggc caggtccaca
     2221 cgcacgatgc cctgggcgaa atcctgctgg ctggaataga cattgacctt gaaggcgggg
     2281 tcggtgccgt ccgtggaggg cacgatggcg ccttccttct gcatctggac gaggaattcg
     2341 acgagcttct gcttgagggc gccgcggcct tcctcgttgt tgaggcgtcc gatgaagagt
     2401 tcgccgatgg tcttgacgcc gcgcacggcg tggtcggcca cgcggcgcac gctgatctgg
     2461 tcgccgtcgg tggtgaggcc gcgcacgacg atgatgccgc ggccgcgctg ggcgacgatg
     2521 gggacgacgt tcttgcgcag gagttcctgc tgggattcga caccgagggc cggggccggc
     2581 tcgcccagcc cgctgagacg cttgaacgtg ggcgactgaa aatagtcgag gctgccggtc
     2641 attccggcca ccgcgcccac cacgccggcc ggggcgacga ggatgaagcg gtcggaga
//
LOCUS       DAOQPA010000234         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_30602_length_2675_cov_16.507634, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000234 DAOQPA010000000
VERSION     DAOQPA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30602_length_2675_cov_16.507634"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="aceE"
                     /locus_tag="PKX01_17285"
     CDS             complement(<1..916)
                     /gene="aceE"
                     /locus_tag="PKX01_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990311.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HMZ77942.1"
                     /translation="MAANTQVVLQSDIDAQETREWLEALEGVVTQEGPERAHFLIEKL
                     IEAGREEGIDIPYSANTQYINTIPADQQPRYPGDPDMEIKLHSYIRWNAMAMVVRANK
                     HTNVGGHIASFASAAALYDVGFSHFWKSIDHDTGGDLIYFQGHSVPGVYSRAYMLGRL
                     TDEQMDNFRQESDGQGISSYPHPWLMPDFWQFPTVSMGLGPICAIYSARFMKYLASRG
                     LIDAAKAEQRKVWAFLGDGETDEVESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRG
                     NGKIIQELEAEFRGAGWNVIKLIWGTHWD"
     gene            complement(1309..2484)
                     /locus_tag="PKX01_17290"
     CDS             complement(1309..2484)
                     /locus_tag="PKX01_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77943.1"
                     /translation="MGVGLGLALAGAGAGAVEPIRIGVTGPFTGGSSPMGVAMRDGIR
                     LAAQELNEKGGILGRPLALVERDDEARPERGAQVVQELINHEKVVAGLGIVNTGVALA
                     SQRFYQQAHLPMITSVATGTIVTRQFLPPQYPDNYIFRVSAPDSVQATMIVEEAVERR
                     RFRKLAILHDSTNYGQLGREDLERALERHGIRPVAVERFNIRDVDMTAQLTRARAAGA
                     EAILTYGLGPELAHIANGTVRIGWKVPLIGSWALAMSNFIDNAGPNAEGTRMPQTFIQ
                     EGDTPRRKDFIDRYRKAFGVERIPVAPAAAQGYDSMLVLAAAIRQARSTDGPKVREAL
                     ETLKERVEGIVMVYDRPFSRQDHEAIGSTRIPVIGEVRNSQVVFAYDEDRKRGAQHK"
BASE COUNT          394 a          877 c          945 g          459 t
ORIGIN      
        1 cgtcccagtg ggtgccccag atcagcttga tgacgttcca gccggcgccg cggaactcgg
       61 cttcgagctc ctggatgatc ttgccgttgc cgcgcaccgg gccgtccagg cgctgcaggt
      121 tgcagttgat gacgaagatc aggttgtcga gcttttcgcg gccggccatg ccgatggcgc
      181 cgagggactc gacttcgtcg gtctcgccgt cgccgaggaa ggcccacacc ttgcgctgct
      241 cggccttggc ggcgtcgatg aggccgcggc tggcgaggta cttcatgaag cgcgcggagt
      301 agatcgcgca gatcgggccg aggcccatgg agacggtggg gaactgccag aagtccggca
      361 tcagccaggg gtgcgggtag gacgagatgc cctggccgtc ggattcctgg cggaagttgt
      421 ccatctgctc gtcggtgagg cggccgagca tgtaggcgcg ggaatacacg ccgggcaccg
      481 agtggccctg gaagtagatc aggtcgccgc cggtgtcgtg gtcgatgctc ttccagaagt
      541 gggagaagcc cacgtcgtag agggccgcgg cagaggcgaa ggaggcgatg tggccgccca
      601 cgttggtgtg cttgttggcg cgcaccacca tggccatggc gttccagcgg atgtaggagt
      661 ggagcttgat ctccatgtcc gggtcgccgg gataacgggg ctgctggtcg gccgggatgg
      721 tgttgatgta ctgggtgttg gcggagtagg ggatgtcgat cccttcctca cggccggctt
      781 cgatgagctt ctcgatcagg aagtgagccc gttcggggcc ttcctgggtc acgacgccct
      841 cgagcgcctc cagccattcc cgggtttcct gggcgtcgat gtccgactgc aggacgactt
      901 gcgtgttcgc ggccatggat tttgtctcct catgacttgc gggttgtgga tggccgccgt
      961 gggtaacggc ggcggatttt ttcactgcaa acctgcgggg ctggatcaca gcccgttttt
     1021 cgtaatataa aaccaaatct cgcgatgtgc aataagggtc aaccgcaatg ggggtgatga
     1081 cgccccgtgc gacgcccgca cgaacgccac cgcgcggccc cgaggacggg caggcgtgta
     1141 gacgccggcg ggcccgccgt ggggtcgtgc ggtgactgcc ggccggcgct tgacgcgcac
     1201 ggccggcggc ccttcattcg atagggaggg aaaaagacgg ccgggggaga gggagggagg
     1261 gagagaaccc ccggccggtt gcggaaaatg gctgatccgc gggcggggtc atttgtgctg
     1321 cgcgccccgc ttgcgatcct cgtcatacgc gaagaccact tggctattgc gaacttctcc
     1381 gatcacgggg atccgcgtgc tgccgatcgc ttcgtgatcc tgcctggaga aggggcgatc
     1441 atataccatc acgatgccct cgacgcgctc cttcagcgtt tccagcgcct cgcgcacctt
     1501 gggtccgtcg gtgctgcggg cctgtcggat ggcggcggcg agcaccagca tggagtcgta
     1561 gccctgggcc gcggcgggcg ccaccgggat gcgctccacg ccgaaggcct tgcggtagcg
     1621 gtcgatgaaa tccttgcgac gcggcgtgtc cccctcctgg atgaaggtct ggggcatgcg
     1681 cgtcccctcc gcgttggggc ccgcgttgtc gatgaagttg gacatggcca gcgcccagct
     1741 gccgatcagc ggcaccttcc agccgatgcg caccgtgccg ttggcaatgt gggccagctc
     1801 cggccccagg ccgtaggtga ggatcgcctc tgccccggcg gcgcgggcgc gggtgagctg
     1861 ggcggtcatg tccacgtcgc ggatgttgaa gcgctccacc gccaccgggc gaatgccgtg
     1921 ccgctccagc gcccgctcca ggtcctcgcg gccgagctgg ccgtagttgg tgctgtcgtg
     1981 gaggatggcc agcttgcgga agcggcgccg ctccactgcc tcctcgacga tcattgtggc
     2041 ctgcacgctg tccggcgccg acacgcggaa gatgtaattg tccggatact ggggcggcaa
     2101 gaattgccgg gtgacgatgg tcccggtggc cacggaggtg atcatcggca ggtgcgcttg
     2161 ctgatagaag cgctggctcg ccagggccac gccggtgttc acgatgccca gcccggccac
     2221 caccttctcg tggttgatga gctcctgcac cacctgggcg ccgcgctccg ggcgcgcctc
     2281 gtcgtcccgc tccaccagcg ccagcgggcg cccgaggatg ccgcccttct cgttgagctc
     2341 ctgggccgcc aggcggatgc cgtcgcgcat cgccacgccc atcgggctcg acccgccggt
     2401 aaacgggccg gtcacgccga tcctgatcgg ctccaccgcg cccgccccgg cgccggccag
     2461 ggccagcccg aggcccacgc ccacacgccg cccccaggcc cccagcctcc gcaggaatcc
     2521 cgtcacgccc gtcctccccg ttggccgccg cacgcccatc cgcgccggcc cggcccccgc
     2581 acgccaccct agcgcggcgc ggggagcggg ggaatacgcg attaccctta gcgctgaagg
     2641 gccgcgaaga acggtgaacg gcggaaaccc ttcac
//
LOCUS       DAOQPA010000235         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_31220_length_2645_cov_16.996911, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000235 DAOQPA010000000
VERSION     DAOQPA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31220_length_2645_cov_16.996911"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKX01_17295"
     CDS             complement(<1..249)
                     /locus_tag="PKX01_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HMZ77944.1"
                     /translation="MSRAEMDALGWDACDVILVTGDAYIDHPSFGMALVGRALEAQGF
                     RVGIISQPDWHSAEPFRALGRPRLFFGVTAGNMDSMINR"
     gene            complement(392..1177)
                     /locus_tag="PKX01_17300"
     CDS             complement(392..1177)
                     /locus_tag="PKX01_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ77945.1"
                     /translation="MKEHTPPAPDTPLSVLIVDDEAPARERLRDLLGDIAERHPTRLV
                     GMAANGIEALGLLDTTPADLILTDIRMPAMDGIELARHTARLDPPPAVIFTTAYDEYA
                     VRAFELAAVDYLLKPVRAARLAEALAKARRLHPPADTVLRQIATAGRSHFSVSERGRI
                     LLVPVPEVLYLKAEQKYVVARCVDREYLLDESLVQLEEEFPERFLRIHRNCLIARHAV
                     RGVARATTSEDGEAHWVILLDGLPDQLPVSRRQWPTVKDALGV"
     gene            complement(1174..2190)
                     /locus_tag="PKX01_17305"
     CDS             complement(1174..2190)
                     /locus_tag="PKX01_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ77946.1"
                     /translation="MSIKQNPGRTGRRHDGPLPDFRNLGVVLRVLVGVNGLGLIAVLA
                     RSGQWSSLGGALMDMAMCVEPPLIVAVAALFLLQPVLARMPAPAGWVGVGAVALAAAA
                     LFNTLLAPLDVGDLASWLGWTALLVVAVRAYFRYLAAVYAPSLAEARLLALTARIRPH
                     FLFNSLNGVLGILRSDPRRAEAALEELADLFRALMRENRDLVPLADEIALCERYLALE
                     HLRLGPRLSVRWDCAPDVGTAQVPPLFLQPLVENAVYHGIEPGAGPGEVAIAIRRHGP
                     ELRIEIVNPLQGGHSHPGGNRMAVDNIRERLMLFFDLEAGLETEQADGLHRVRIRLPY
                     RTAT"
     gene            2279..>2645
                     /locus_tag="PKX01_17310"
     CDS             2279..>2645
                     /locus_tag="PKX01_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMZ77947.1"
                     /translation="MADQSAPQDGAAKAWSGRFSEPVSDLVKRYTASVFFDNRMAAQD
                     IRGSLAHARMLARQGIIGAQDLADIERGMAQIAGEIERVEFAWNLDDEDVHLNIEKRL
                     TALVGDAGKRLHTGRSRNDQ"
BASE COUNT          446 a          873 c          969 g          357 t
ORIGIN      
        1 gcggttgatc atcgagtcca tgttcccggc ggtgacgccg aagaacaggc gcggccggcc
       61 gagggcgcgg aagggctcgg cggaatgcca gtcgggctgg ctgatgatgc caacgcggaa
      121 gccctgggcc tccagggcgc ggccgaccag ggccatgccg aagctgggat ggtcgatgta
      181 ggcgtcgccg gtgacgagga tgacgtcgca ggcatcccag ccgagggcgt ccatctcggc
      241 ccgggacatg ggcaggaagg gcgcgggacg caggtccggc cgatggcgcg gataggaggg
      301 cagcaggcgg ggcgtgtttt ccatgggcgc gattgtacgg gagcccccgt tggaacaccc
      361 gccgccaagg acgttcccga cccgcggcgg ctcacacgcc gagggcgtcc tttacggtgg
      421 gccactggcg tcggctcacc gggagctggt cgggcaggcc gtccaggagg atcacccagt
      481 gggcctcacc gtcctcgctg gtggtggcgc gggccacgcc gcgcaccgca tggcgggcga
      541 tcaggcagtt gcggtggatg cgcaggaagc gctcggggaa ttcttcctca agctgcacga
      601 gggattcgtc cagcaggtat tcccggtcga cgcagcgggc caccacgtat ttctgctcgg
      661 ccttgaggta gagcacctcg ggcaccggca cgaggaggat gcggccgcgt tcgctgacgc
      721 tgaaatggct gcgcccggcc gtggcgatct ggcgcagcac cgtgtcggca ggcggatgga
      781 ggcggcgggc cttggccagg gcctcggcca gcctcgcggc gcggaccggc ttgaggaggt
      841 aatccaccgc agccagctcg aaagcgcgca ccgcgtattc gtcgtaagcg gtggtgaaga
      901 tgaccgccgg cggcggatcg aggcgggccg tgtggcgggc cagctcgatg ccgtccatgg
      961 ccggcatgcg gatgtcggtg aggatcaggt cggccggcgt cgtgtcgagc agacccaggg
     1021 cctcgatgcc attggcggcc atgcccacga ggcgcgtcgg atgacgctcg gcgatgtcgc
     1081 cgaggaggtc acgcaggcgc tcccgggcgg gcgcctcgtc atccacgatg aggacggaga
     1141 gcggggtatc gggggcggga ggggtgtgtt ccttcatgtc gcagtgcggt aaggcagtcg
     1201 gatgcggacg cggtggaggc catccgcctg ttcggtctcc aggccggctt ccaggtcgaa
     1261 gaagagcatc aggcgttcgc ggatattatc cacggccatg cggttgccgc caggatggct
     1321 gtgaccaccc tgcagcggat tgacgatctc gatgcgcagt tcgggaccgt gacgccggat
     1381 cgcgatggcg acctcacccg gtccggcacc cggctcgatg ccgtgataga cggcgttttc
     1441 gacgaggggc tggaggaaca ggggcggaac ctgcgccgtc ccgacgtccg gggcgcaatc
     1501 ccagcgcacg ctcaggcgcg ggccgaggcg caggtgctcc agggccagat agcgctcgca
     1561 cagcgcgatc tcgtcggcca gcggcaccag atcgcggttt tcgcgcatga gggcgcgaaa
     1621 caggtcggca agctcctcga gggccgcctc ggcccggcgc ggatcggagc gcaggatgcc
     1681 gagcaccccg ttgaggctgt tgaacaggaa atggggccgg atgcgcgccg tcagggcgag
     1741 caggcgcgcc tcggcgaggg acggggcata gaccgccgcg agataacgga agtaggcccg
     1801 cacggctacc acgagcaacg ccgtccagcc cagccagctc gccaggtcgc ccacgtccag
     1861 cggcgcgagc agggtgttga acaaggctgc ggccgccagg gccacggcgc cgacaccgac
     1921 ccagcctgcc ggcgctggca tccgcgccag cactggctgg agcaggaaca gggcggccac
     1981 cgccacgatc aggggcggct cgacgcacat ggccatgtcc atgagcgctc cgccgaggct
     2041 cgaccactgc ccgctgcggg ccagcacggc gatcagcccg aggccattca cgcccaccag
     2101 gacgcgcagc accaccccga gattgcggaa atccggcaac ggcccgtcgt gccggcggcc
     2161 ggtgcggccg gggttttgct ttatactcat gaatcaaaaa gaaaccgggg caagaccttg
     2221 aatctgcggg catccccgcg gcagggcgcc ccctcgaacc gctgagagca ttccaattat
     2281 ggcagatcaa tccgccccgc aggacggcgc cgccaaggcc tggtccgggc gcttctccga
     2341 acccgtttcc gatctggtca agcgctacac cgcgagcgtg ttcttcgaca accggatggc
     2401 cgcccaggac atccgcggct ccctcgccca cgcccgcatg ctggcccgcc agggcatcat
     2461 cggcgcccag gacctggccg acatcgagcg cggcatggcc cagatcgcgg gtgagatcga
     2521 gcgcgtcgaa ttcgcctgga acctggacga cgaagacgtc cacctcaaca tcgagaagcg
     2581 cctcaccgcc ctggtgggcg acgccggcaa gcgcctgcac accggccgca gccgcaacga
     2641 ccagg
//
LOCUS       DAOQPA010000236         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_31555_length_2628_cov_23.495919, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000236 DAOQPA010000000
VERSION     DAOQPA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31555_length_2628_cov_23.495919"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKX01_17315"
     CDS             <1..198
                     /locus_tag="PKX01_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77948.1"
                     /translation="MEGQKGMSGMSGMQGQKGMSGMSGMEGQKGMSGMSGMSGMSGNQ
                     HKKHKQQGMSGMSGMSGMEGK"
     gene            275..1102
                     /locus_tag="PKX01_17320"
     CDS             275..1102
                     /locus_tag="PKX01_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77949.1"
                     /translation="MVVTRRIKQYLRLLATLAAAWTLGPALAAEGHDATGVDYQRARY
                     SPLHFRPAIQQASNEQCLACHSEILTRRPRERSPAGVTAASAKAWYQQLSTYQGAQAD
                     FHRRHLDTGLGKSLMNLQCNTCHDGVDPRDKHPLSSATSPRDGGFRMRKTVMPETTCL
                     KCHGQMNWQVMGLPGPWKESREMFQNNCLLCHEGIRTQRHQVNYLKPEAIETAGKESG
                     DACYGCHGGRAWYRITYPYPRHPWPGMAEETPDWAKGRPTRSEARFVTGASEKGQQP"
     gene            1099..>2628
                     /locus_tag="PKX01_17325"
     CDS             1099..>2628
                     /locus_tag="PKX01_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ77950.1"
                     /translation="MNSVLDAGVTPSATETTRRDFLKVMGVGAGLAGLGGLTMTPKDA
                     KAFAYEPYYTDDQLTTVVTSCAHNCGSRHMLVAHKKGDVIVRLSSDDGRYQKGGFFGK
                     DTVDEPQLRACLRGRSYRSRLYSPERLLYPMMRVGKRGEGKFKRVSWDEALDFVARKM
                     VELKKTYGPTALLDQSYAGASYGVLHKSDQIEGLLGRFLGMFGCRTNSWSVPSYQGTT
                     FSSRITFGTIEDGNEDDAYAHSKLIIMWGWNPAYTFHGGNTFYYMRMAKQRGCKFVLV
                     DPQYTDSASAYDAWWIPIRPNTDAAMLAGMAHVIFTENLHDQAFIDKFVLGMDAGTMP
                     KDYADAESFKDYILGTRDGTPKSPEWAEAICGVSADDIRKLARLYATTKPAALKASWA
                     PGRAAYGEQYNRMAAAVQAMTGNIGKLGGCAEGVGKAWHSESVAYPYDEYANVWYASI
                     KSDRWAHCVINYPKLKREDVGLWPREDELDGQIPNIRGIFWQGSDWFNQLTNINKEIQ
                     AI"
BASE COUNT          516 a          885 c          829 g          398 t
ORIGIN      
        1 atggaaggcc agaagggcat gagcggcatg tccggcatgc agggccagaa gggcatgtcc
       61 gggatgtcgg gcatggaagg ccagaaaggc atgtccggca tgtctggtat gagcggcatg
      121 tccggcaatc agcacaagaa gcacaagcag caaggcatga gcggcatgtc cggcatgtcg
      181 ggcatggaag gcaagtaagc cttcttccgg atcccggctt cggccgggat cctttttgcc
      241 ggccgtgtgg ccggacgcaa tgcacgaggc tgccatggtg gtcacaagaa gaatcaagca
      301 gtacctgcgc ctgctggcaa cgctggccgc ggcctggacc ctgggtccgg ccctcgccgc
      361 cgaaggtcac gatgcgaccg gagtcgatta tcagcgtgcc cgatattccc cgctccactt
      421 ccggcccgcg atccagcagg ccagcaatga acaatgtctc gcctgtcaca gcgagatcct
      481 gacccgcagg ccgcgggaac gctcgccggc gggtgtcacc gcggcctcgg ccaaggcgtg
      541 gtaccagcag ctgtccacct accagggtgc gcaggccgat ttccatcgtc gtcacctgga
      601 taccgggctc gggaaaagcc tgatgaacct ccagtgcaac acctgtcacg acggtgtcga
      661 cccccgcgac aaacatcccc tcagctccgc cacctccccg cgcgatggtg gtttccgcat
      721 gcgcaagacg gtgatgcccg agaccacctg cctgaagtgt catggccaga tgaactggca
      781 ggtcatgggg ctgccggggc cgtggaagga gagtcgcgag atgttccaga acaactgcct
      841 gctgtgccac gagggcatcc ggacccagcg gcaccaggtg aactatctga agcccgaagc
      901 catcgagacg gccgggaagg agagcggtga cgcctgctac ggctgccacg gcggccgggc
      961 gtggtaccgc atcacctacc cctatccgcg tcatccctgg ccgggcatgg cggaggagac
     1021 gccagactgg gcaaaggggc ggccgacccg ttccgaagcc cgcttcgtga cgggtgccag
     1081 cgaaaaagga caacaaccat gaacagcgtg ctcgatgcgg gcgtcacacc gtctgcgacg
     1141 gagacgaccc gacgggattt cctgaaggtg atgggcgtcg gggccggact cgccggcctg
     1201 ggcgggctga cgatgacgcc gaaagacgcc aaggccttcg cctacgaacc ctattacacc
     1261 gacgaccagc tcactacggt ggtcacctcc tgcgcccata actgcgggtc acgccacatg
     1321 ctggtggccc acaagaaggg cgacgtgatc gtgcgcctgt cctccgacga tggccgttac
     1381 cagaagggcg gcttcttcgg caaggacacg gtggatgagc cccagctgcg cgcctgcctg
     1441 cggggccgct cctaccgttc gcgcctgtat tcgccggagc gcctgctcta tcccatgatg
     1501 cgcgtcggca agcgcggcga gggcaagttc aagcgggtgt cctgggacga agccctggat
     1561 ttcgtcgccc gcaagatggt cgagctgaag aagacctacg gccccacggc gctgctcgac
     1621 cagagctacg cgggcgcctc ctacggcgtg ctccacaagt ccgaccagat cgaaggcctc
     1681 ctcgggcgct tcctgggcat gttcggctgt cgcaccaatt cctggtcggt gccgagctac
     1741 cagggcacca ccttctccag ccgcatcacc ttcggcacca tcgaggacgg caacgaggac
     1801 gacgcctacg cccacagcaa gctcatcatc atgtggggct ggaacccggc ctacaccttc
     1861 cacggcggca acaccttcta ctacatgcgc atggccaagc agcgcggctg caagttcgtg
     1921 ctggtggacc cgcaatacac cgactcggcg tccgcctacg acgcctggtg gatcccgatc
     1981 cggcccaata ccgacgccgc catgctggcc ggcatggccc acgtgatctt caccgagaac
     2041 ctccacgacc aggccttcat cgacaaattc gtgctgggca tggatgccgg cacgatgccc
     2101 aaggactacg ccgacgcgga gagcttcaag gactacatcc ttggcacccg cgacggcacg
     2161 cccaagtcgc cggaatgggc cgaggccatc tgcggcgtgt cggccgacga catccgcaag
     2221 ctggcgcgcc tgtacgccac caccaagccg gccgccctca aggcgtcctg ggcgcccggc
     2281 cgggccgcct acggcgaaca gtacaaccgc atggccgccg cagtgcaggc catgaccggc
     2341 aacatcggca agctgggcgg ctgcgccgag ggcgtgggca aggcctggca ttcggagtcg
     2401 gtggcctatc cctacgacga atacgccaac gtctggtacg cctccatcaa gtcggaccgc
     2461 tgggcccact gcgtgatcaa ctaccccaag ctgaagcgcg aggacgtggg gctgtggccg
     2521 cgggaggatg aactcgacgg ccagattccc aacatccgcg gcatcttctg gcagggctcc
     2581 gactggttca accagctcac caacatcaac aaggagatcc aggccatc
//
LOCUS       DAOQPA010000237         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_31660_length_2622_cov_14.324893, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000237 DAOQPA010000000
VERSION     DAOQPA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31660_length_2622_cov_14.324893"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1020)
                     /gene="istB"
                     /locus_tag="PKX01_17330"
     CDS             complement(211..1020)
                     /gene="istB"
                     /locus_tag="PKX01_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HMZ77951.1"
                     /translation="MRELGAELKALRLYGMASTWAELSANGGDVGLQSARWLIERLLE
                     AESTDRAMRSVRYQMSAARFPVHRDLAGFDFGQSCVERKLITALADLSFTDQAHNVVF
                     IGGPGTGKTHLATALGVSGITRHGRRVRFYSTVDLVNALEQEKAAGKAGRLALSLRSM
                     DLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVMITTNLTFGEWASVFGDAKMTTAL
                     LDRLTHHCHIVETGNESWRFQHSSAAAKSRIQSREKSKRVSASDSDEADPF"
     gene            complement(1013..2509)
                     /gene="istA"
                     /locus_tag="PKX01_17335"
     CDS             complement(1013..2509)
                     /gene="istA"
                     /locus_tag="PKX01_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HMZ77952.1"
                     /translation="MELLGKVRRMHYRDGLSRSEIARRTGLSRNTVKKWLTAPEAVEP
                     RYRRVQAPGKLDPFHGALLQALEADARRPKRDRRTAKALYAELKSQGYAGGYTVLTDF
                     IRHWRVQAGANAPTRAFVPLRFELGEAFQFDWSEERLVIGGVWRKLLVAHLKLCASRA
                     FVLIAYPSQSHEMLFDAHTRSFQALGGIPRRGIYDNMKTAVDKVKKGKGRVVNARFSA
                     LCSHYLFDPDFCNVASGWEKGVVEKNVQDSRRRIWLEAGTLRFGSFAELNAWLLARCQ
                     ALWQEVRHPEYALSVAEMLEHEQPHLMPMVSAFDGYVEEPDRVSSTCLVTAARNHYSV
                     PCELAGKLISKRFYPERIEIVFDEVVMASHPRLFERGQTRYDWRHYLPLVERKPGVLR
                     NGAPFAEMPEPLVRLQRLLLRREGGDRVMSQMLAVVPKAGLDTVLVAVELVLESGVVS
                     VEHVLNVIARLNQAPLPESVPTTLQVKEAPVADTGRYDRLRTQEADHA"
BASE COUNT          469 a          886 c          797 g          470 t
ORIGIN      
        1 tgtcggcgcc ggtccaatat tgatcaggtg taccggttga attttgacca ggggcgatag
       61 ctgctcgtca ttgctgcagc tgtggataag tgtatcggat tcgcggtcct gtttgccctc
      121 ctgtgtgctt gctgtcgagc gagggagagg cgacgcgggg gaatctgcga agggcgtagc
      181 ccgaagcaga ttcccccgcg tcgcgccggc tcagaacggg tcagcctcgt cgctatcaga
      241 cgctgaaacc cgcttgctct tctccctcga ctggatgcgg gacttggcgg ccgcgctgct
      301 gtgctggaag cgccacgact cgttaccggt ctccacgatg tggcagtgat gcgtcagccg
      361 gtccagcagc gccgtcgtca tcttggcgtc gccgaacacg ctggcccatt ccccgaaggt
      421 cagattggtc gtgatcatca cgctggtgtg ttcgtagagc ttggagagca ggtggaagag
      481 caaggcgccg ccggcttggc tgaaggggag atagcccaac tcgtcgagga tgaccaggtc
      541 catgctgcgc agcgacagcg caagccgccc ggctttgcca gcggccttct cctgttcgag
      601 cgcattgacc agatcgactg tcgagtaaaa gcgcactcgc cggccatgac gcgtaatccc
      661 tgagacgccc agcgccgttg ccagatgggt cttgcccgtg cccggcccgc cgatgaagac
      721 gacgttgtgc gcctggtcgg taaacgacag atcggccagt gctgtgatga gcttgcgctc
      781 aacgcacgac tgcccgaagt cgaaacccgc cagatcccga tgaacgggga agcgggcggc
      841 cgacatctgg tagcggaccg agcgcatggc gcggtcggtg gattcggcct ccaacaagcg
      901 ctcgatgagc cagcgcgcgc tctggagccc gacgtcaccg ccattggcgc tcaactcggc
      961 ccaggtgctg gccatgccat atagacgcaa ggctttcagt tcggcgccga gctcacgcat
     1021 gatcagcctc ctgggtacgc aggcggtcgt agcgaccggt gtcggccacc ggcgcctcct
     1081 tgacctgcag cgtggtcggc accgattcgg gcaacggcgc ctggttgagc cgggcaatga
     1141 cattgagcac atgctccaca ctgaccacgc ccgattcgag caccagttcg acggcgacga
     1201 gcaccgtgtc gagcccggcc ttgggtacca ccgcaagcat ctggctcatc acccggtcac
     1261 cgccttcgcg ccgcagcagc aggcgttgca gccgcaccag cggctcgggc atctcggcaa
     1321 acggcgctcc gttcctcaac accccgggct tgcgctccac cagcggcagg tagtggcgcc
     1381 agtcgtagcg ggtttgcccc cgctcgaaca ggcgcgggtg acttgccatc acgacctcgt
     1441 cgaacacgat ctcgatgcgc tccggataga accgcttgct gatcagcttg cccgccagct
     1501 cgcacggcac cgagtagtgg ttgcgggcgg ccgtcaccag acaggtgctc gacacgcggt
     1561 ccggctcttc gacataccca tcgaatgcag acaccatcgg catcaggtgc ggttgctcgt
     1621 gttcgagcat ctcggccacc gacaacgcgt actcgggatg acgcacttcc tgccacaggg
     1681 cctggcaacg ggccagcagc caggcgttca gctcagcaaa gctgccaaag cgcaacgtgc
     1741 cggcctccag ccagatccgc cgccggctgt cctgcacgtt cttctcgacg acacctttct
     1801 cccatcccga ggcgacgttg cagaagtccg gatcgaacag gtagtgcgag cacagcgccg
     1861 agaagcgtgc attgaccacc cggcccttac ccttcttgac cttgtcgacc gccgtcttca
     1921 tgttgtcgta gatgccgcgc cggggaatcc cgcccagcgc ctggaaactg cgtgtgtggg
     1981 cgtcgaacag catctcgtga ctttggctcg ggtaggcgat cagcacgaag gctcggctcg
     2041 cacacaactt caggtgcgcc accagaagct tccgccagac cccgccgatc accagccgct
     2101 cttcgctcca gtcgaactgg aacgcttcgc ccagctcgaa cctcagcggc acaaaagcgc
     2161 gcgtcggcgc gttcgcgccc gcctgcacac gccagtggcg gatgaaatcg gtcaggaccg
     2221 tgtaaccgcc ggcatagccc tgcgatttca gttccgcgta aagcgccttc gccgtacgcc
     2281 ggtcgcgctt cgggcgccgc gcatcggctt ccagcgcctg caacagcgca ccgtggaagg
     2341 ggtccaactt gcccggtgct tgcacgcgcc gataacgcgg ctcaacggcc tcaggggccg
     2401 tcagccactt cttcaccgtg ttgcgcgaca gccccgtgcg ccgcgcaatc tccgaacggg
     2461 acagcccgtc ccggtaatgc atccgtctga ccttccccaa cagttccatg gtgatcacct
     2521 catgtcctct gcctcaaaat gaggcagaga ggttaaacac cctggtcagt cttcagccgg
     2581 aattcacccc gtcagatggt cagttttcgg ccggcgccaa ca
//
LOCUS       DAOQPA010000238         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_31933_length_2609_cov_14.907987, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000238 DAOQPA010000000
VERSION     DAOQPA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31933_length_2609_cov_14.907987"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="gatB"
                     /locus_tag="PKX01_17340"
     CDS             complement(<1..440)
                     /gene="gatB"
                     /locus_tag="PKX01_17340"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HMZ77953.1"
                     /translation="MSRTDWEVVIGLEIHTQLNTASKIFSGASTAFGAAPNVQASAVD
                     IALPGVLPVLNRGAVERAIRFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYELPV
                     VQGGAITLLVPDGQGGHYEKRVRLTRAHLEEDAGKSLHEDFHGMS"
     gene            complement(461..961)
                     /locus_tag="PKX01_17345"
     CDS             complement(461..961)
                     /locus_tag="PKX01_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77954.1"
                     /translation="MFHRFFPSLLAALIAGCASVELPRLPGARDEPPAARVPAAPSAD
                     SGKLKPMPLRTFTVSTQCSFKDETGNFGRATVDVQDSKVRALAVELVMPRRGTCRYDL
                     TQFRQTQALPSVELAGGRDCRIRMWEQGETITVSFANCPQHCNPASATDYVWPLLIDR
                     PTGQCD"
     gene            complement(967..1338)
                     /locus_tag="PKX01_17350"
     CDS             complement(967..1338)
                     /locus_tag="PKX01_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77955.1"
                     /translation="MPYTRTQIQAFVGGELTTYLPQRDHRILAGCFARDLNPKAPDIV
                     FAGNKAKVAHKARLLARQPDNCFPVFVRDNKRAPYFFQGYFAYERLSDLAEDIRSAEA
                     LSGRYNQLSCVLHLKRCPDRE"
     gene            complement(1368..>2609)
                     /gene="gatA"
                     /locus_tag="PKX01_17355"
     CDS             complement(1368..>2609)
                     /gene="gatA"
                     /locus_tag="PKX01_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMZ77956.1"
                     /translation="IPLAHKDVFCTEGVLTTCASKMLANFVSPYDAHVVSLLKAAGAV
                     MVGKTNMDEFAMGSSNENSHFGPVRNPWNTDTVPGGSSGGSAAAVAARLVPIATGTDT
                     GGSIRQPAALCGITGIKPTYGTVSRYGMIAYASSLDQGGAFARTAEDCAVLLSAMAGH
                     DPRDSTSLERPAEDYGRDLAQPLAGLRIGLPTEFFAEGMSDDVRAVVDAALDQYRALG
                     ATTVEVSLPNARLAIPAYYVIAPAEASSNLSRFDGVRYGHRAAEYTDLNDMYCKSRAQ
                     GFGEEVQRRILVGTYVLSHGYYDAYYIKAQKLRRLIAEDFQSALAQCDVIAGPVSPTV
                     AFGIGEKSDDPVQMYLSDIYTIAVNLAGLPGLSHPAGFGAGGLPVGLQLIGNYFAEAR
                     LLNVAHQFQQATDWHARAPQL"
BASE COUNT          413 a          772 c          983 g          441 t
ORIGIN      
        1 gacatgccgt ggaagtcctc gtgcaggctc ttgccggcgt cttcttccag gtgggcgcgg
       61 gtcaggcgca cacgcttctc gtagtggccg ccctggccat ccggcaccag cagggtgatg
      121 gcgccgccct ggacgacggg cagctcgtac tggctgatct ggtagccctt gggcaggtcc
      181 ggatagaagt aattcttgcg ggcgaagatg ctcctcggcg cgatggtggc gccgatcgcc
      241 agcccgaagc ggatggcccg ctccacggcg ccccggttga gcaccggcag cacgccgggc
      301 agggcgatgt ccacggcgct ggcctggacg ttgggcgcgg caccgaaggc ggtgctggcg
      361 ccggagaaga tcttggaggc ggtgttgagc tgggtgtgga tttccagccc gatgacgact
      421 tcccagtcgg ttctgctcat ggtggttctc gcggtggcgt tcagtcgcac tggcccgtgg
      481 gccggtcgat cagcagcggc cacacataat cggtggcgct ggcgggattg cagtgctgcg
      541 ggcagttggc gaaggagacg gtgatggtct cgccctgctc ccacatgcgg atgcggcagt
      601 cacgaccgcc agccagttcg accgacggca gggcctgggt ctggcggaac tgggtgaggt
      661 cgtagcggca ggtgccgcgc cggggcatga cgagttcgac ggcgagggcg cggaccttgg
      721 aatcctgcac atccacggtg gccctgccga agttgccggt ctcgtccttg aagctgcact
      781 gggtgctcac cgtgaaggtg cgcaggggca tgggcttgag cttgccgctg tcggccgagg
      841 gcgcggccgg cacacgggcg gccggcggtt cgtcccgcgc gccgggcagc cgcggcagct
      901 cgacgctggc gcagccggcg atcagggctg ccagcaggga cggaaagaat ctatgaaaca
      961 cgtctgtcat tctcggtcgg gacaccgctt gaggtgaaga acgcacgaca actggttgta
     1021 tcgaccagac agcgcttccg cggagcggat gtcttctgca agatcgctca ggcgctcata
     1081 cgcgaaataa ccctgaaaga aataaggtgc ccttttgttg tcgcggacaa agaccggaaa
     1141 gcaattgtcg ggttgccggg cgagcaaacg tgccttgtgg gcgacctttg ccttgttgcc
     1201 cgcaaatacg atatccggcg ccttgggatt gagatcacgg gcgaaacagc ctgccaggat
     1261 tcggtgatcc cgttgcggga gatacgttgt caattctccc cccacaaacg cctggatctg
     1321 agtgcgcgta tagggcacgc caaacttcag aggtagcatc gccaccctca cagctgcggc
     1381 gcgcgggcgt gccagtcggt ggcctgctgg aactgatggg ccacgttgag caggcgggct
     1441 tccgcgaagt agttgccgat gagctgcagg ccgacgggca ggccgccggc gccgaaaccg
     1501 gccgggtggg acaggccggg caggccggcg aggttcacgg cgatggtgta gatgtcggag
     1561 aggtacatct gcaccggatc gtcgctcttc tcgccgatac cgaaggccac cgtggggctc
     1621 accggaccgg cgatcacgtc gcactgggcc agcgcggact ggaagtcctc ggcgatgagg
     1681 cggcgcagct tctgggcctt gatgtagtag gcgtcgtagt agccgtggga gagcacgtag
     1741 gtgcccacca ggatgcgccg ctggacctct tcgccgaagc cctgggcgcg gctcttgcag
     1801 tacatgtcgt tgaggtcggt gtattcggcg gcgcggtggc cgtagcgcac accgtcgaag
     1861 cggctcaggt tggagctggc ctcggcgggg gcgatcacgt agtaggcggg gatcgccaga
     1921 cgcgcgttgg gcaggctgac ctcgacggtg gtggcgccga gggcgcggta ctggtcgagg
     1981 gcggcgtcca cgacggcgcg cacgtcgtcg gacatgccct cggcaaagaa ttccgtgggc
     2041 aggccgatgc gcaggccggc gaggggctgg gccaggtcgc ggccgtagtc ttcggcgggg
     2101 cgttccaggc tggtggaatc ccgcgggtcg tggccggcca tggcggagag cagtacggcg
     2161 cagtcttcgg ccgtgcgggc gaaggcaccg ccctggtcga gggacgaggc gtaggcgatc
     2221 atgccgtagc ggctgaccgt gccgtaggtg ggcttgatgc cggtgatgcc gcacagggcc
     2281 gcgggctggc ggatggagcc gccggtgtcg gtgccggtgg cgatgggcac cagccgcgcc
     2341 gccaccgctg ccgccgaacc accggaggaa cctccgggca ccgtgtcggt gttccagggg
     2401 ttgcggacgg ggccgaagtg ggagttctcg ttggacgagc ccatggcgaa ctcgtccatg
     2461 ttggtcttgc ccaccatcac cgcgccggcc gccttgagca ggctgaccac gtgggcgtcg
     2521 taggggctga cgaaattggc cagcatcttc gaggcgcagg tggtgagcac gccctcggtg
     2581 cagaagacgt ccttgtgagc cagcgggat
//
LOCUS       DAOQPA010000239         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_34292_length_2509_cov_16.731051, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000239 DAOQPA010000000
VERSION     DAOQPA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34292_length_2509_cov_16.731051"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..556
                     /locus_tag="PKX01_17360"
     CDS             89..556
                     /locus_tag="PKX01_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HMZ77957.1"
                     /translation="MSLSSLLRSLVLCAGGLAALIPVGVRAQSSMPQVELTVGMFRIE
                     AEVAATLGDRMQGLMQRRQMAANHGMVFVFTEDQRHCMWMKNTLLPLSVAFMDEQGKI
                     LNIEDMEPQTEDNHCAARPARFALEMNKGWFRQKGIAPGTTIRGVAPLAGAGR"
     gene            complement(787..1701)
                     /locus_tag="PKX01_17365"
     CDS             complement(787..1701)
                     /locus_tag="PKX01_17365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ77958.1"
                     /translation="MNDRVPRITFDLDALRSFVTGIELGGFAKAADRVGRSASAVSAQ
                     LKKLEDQIGAPILQKSGRGRILTPTGECFLGYARRLLALNDEAAIAVRGVELSGSVRI
                     GLQEDFGEHLLSRMLGDFARAHPNVTVEVHLARNAALVRRMEEGRLDLALSWDTGADW
                     PYSETLGQLPLCWIGPAATQATDPTPSGPLPLVLFEAPCVMRSAATAALDAAGRAWRV
                     AVSSASLPGIWTAVGAGLGVTVRTRVGLPGHLVVRNDLPALPRVGLVLHRATDPAPEA
                     VKRLADIMVDTVADMGIDRPRHSAGEEP"
     gene            1793..2113
                     /locus_tag="PKX01_17370"
     CDS             1793..2113
                     /locus_tag="PKX01_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ77959.1"
                     /translation="MHAPHTYGRQAMGDEALKLAQLTDDVLFNDVWERPELSKRDRSL
                     VTVAALLALYRLEQLPGHLVRALENGVAREELVEVITHLAFYAGWPAAHSAIQRLNAI
                     PRQG"
     gene            2124..>2509
                     /locus_tag="PKX01_17375"
     CDS             2124..>2509
                     /locus_tag="PKX01_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HMZ77960.1"
                     /translation="MPLVHISLRRGKSSQYLRQIADGVHRALVDAFEVPPDDRFQTLR
                     EFDGEALIVDPHYLGGPRGADFVLVEITAGRPRSTATKAAFYRRLAEGLQASPGLHPA
                     DLMVVITTTQRDEWSFGHGRATLLQP"
BASE COUNT          363 a          804 c          895 g          447 t
ORIGIN      
        1 gggtgaccgg gccgcgcccg gccctatgcc ggacggggct ggctgggcta tcctcgcggc
       61 tcctttgttg cctgccggat gtccttccat gtcgctgtcg tccttgctgc gttccctcgt
      121 gctgtgtgcc ggggggcttg ccgctctgat tcccgtgggc gttcgggccc agtcatccat
      181 gccccaggtg gagctcacgg tggggatgtt tcgcatcgag gcggaggtgg cggccacgtt
      241 gggggaccgc atgcagggcc tgatgcagcg ccggcagatg gcggccaatc acggcatggt
      301 gtttgtcttc accgaagacc agcggcactg catgtggatg aagaacacgc tgctgcccct
      361 gtcggtggcc ttcatggacg agcaggggaa gatcctcaac atcgaggaca tggagcccca
      421 gaccgaggac aaccactgcg ctgcccgccc ggcccgtttt gcgctggaga tgaacaaggg
      481 ctggttccgc cagaagggga ttgcgccggg cacgacgatc cgcggcgtgg cgccgctggc
      541 gggcgcgggg cgctgagggc gcacgctgtt tgcagcggtc cgcggcggtc gccggcaccc
      601 gtttgatgtg ttcggacagg atggggacgg gggctggcgg ggcgcgcggg ggctacgtcc
      661 gcgtggcgtg cgcctgattg gtgcctgggc gggtgacgcc tgcgcccgcc gcgtcgaggc
      721 tttcgtaggt cgggggcgtt cacgtgtgcg cccggcggct cctccgccat cgtcgggcgg
      781 cgcgtttcag ggttcttcgc ctgcggagtg ccggggtctg tcgatgccca tgtcggcgac
      841 cgtgtccacc atgatgtcgg ccaatcgctt gaccgcttcc ggcgccgggt cggttgcgcg
      901 atggagtacg agcccgaccc gtggcagcgc gggcaggtcg ttgcgtacga cgagatggcc
      961 gggcaggccg acgcgggtgc gtaccgtgac gcccaatccg gcgcccacgg ccgtccagat
     1021 accgggcaga ctggcgctgg acacggcgac gcgccacgcc cggccggcgg cgtcgagtgc
     1081 cgccgtggcg gcgctccgca tcacgcacgg tgcttcgaag agcaccaggg ggagggggcc
     1141 cgagggggtc gggtccgtcg cctgcgttgc ggccgggccg atccagcaca gggggagttg
     1201 tccgagcgtc tcgctgtacg gccaatccgc gccggtgtcc cagctcaggg ccagatcgag
     1261 acgcccttcc tccatccggc gtaccagcgc tgcgttgcgc gcgagatgga cttcgacggt
     1321 cacattcgga tgggcccgcg caaaatcgcc gagcatgcgt gacaggaggt gttcgccgaa
     1381 atcttcctgc aggccgatgc gtacgctgcc cgacagttcg acgccgcgca cggcgatggc
     1441 cgcttcgtcg ttgagggcga gcaggcgccg cgcatagccg agaaaacatt ccccggtggg
     1501 cgtcaggatc cgtccgcgcc cggacttttg caggatgggc gcgccgatct ggtcctccag
     1561 cttcttcaat tgggcactga cggcggacgc cgagcgcccc acgcggtcgg cagccttggc
     1621 aaagccgccg agttcgatgc ccgtgacgaa actgcgcaat gcgtccaggt cgaaggtgat
     1681 gcgggggact ctatcgttca tttttgttga actatcgatt caaatgtttc agatagacgg
     1741 gatgatggtg cggaaatatt ctggcgtcgt caaacgtttc caggagaaca ccatgcatgc
     1801 cccgcacact tatggccgtc aggcgatggg cgatgaagcc ctcaagctgg cccaattgac
     1861 cgacgacgtg ctgttcaacg acgtgtggga gcgtccggaa ctgtccaagc gcgaccgttc
     1921 gctggtgacc gtggcggcct tgctcgcgct gtaccgcctg gagcagttgc ccggccatct
     1981 ggtccgggcg ctggagaatg gcgtcgctcg ggaagagctg gtcgaagtca tcacccatct
     2041 ggccttttac gcgggctggc cggcggccca ttcggccatc cagcggctga acgccattcc
     2101 gcgtcaagga tgaggtgccc accatgcccc tcgtccatat cagtctgcgt cgtggcaagt
     2161 cgtcccaata cctgcggcag atcgccgacg gcgtgcatcg ggcgctggtg gacgcctttg
     2221 aggtgccgcc cgacgaccgc ttccagacgc tgcgggagtt cgatggggag gccttgatcg
     2281 tcgatcccca ttatctcggc ggcccccgcg gcgcggattt cgtgctggtg gagatcaccg
     2341 cgggccggcc gcgcagcacg gcgaccaagg cggccttcta ccgccgtctg gccgagggcc
     2401 tgcaggccag ccccggtctg catccggcgg atctgatggt ggtcatcacc accacccagc
     2461 gggatgaatg gtccttcggt cacggccgcg cgacgctgct ccagcccga
//
LOCUS       DAOQPA010000240         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_34805_length_2490_cov_7.415195, whole genome shotgun sequence.
ACCESSION   DAOQPA010000240 DAOQPA010000000
VERSION     DAOQPA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34805_length_2490_cov_7.415195"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            503..1852
                     /locus_tag="PKX01_17380"
     CDS             503..1852
                     /locus_tag="PKX01_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ77961.1"
                     /translation="MDLKRSLLTRISLFALLIFIVAVAGVLAQTRAAARAHIARHGRT
                     IQQLVADEVARSSDAYHRNIEDLDLSGLRAIGELIHFCVAIENLEARPVTGRCFSDAG
                     EAPALLRGAMARLIGDDVIYRGGLGYYPGIKMGEMTVRPDVDSAAADAWRQIRLILAI
                     TVGILFLNFLVYRPVRRALRPTDAILAVLGRMEAGDLTARMPSFELIELNRIGRVFNL
                     MAERLQQTVGEHQRLAGHVLSVREEERRHLARELHDELGQCLTSINAEAAYAHERARD
                     DLPDLQPCTEAIVRLTGQMMGALQQILRALRPIGLEEFGLRVCLEQLIEGWNRRSRGR
                     CSYRLDWDGDGAGWPDRLNVSLFRIVQESLTNATRHGAASEVTVRLTQTDATVHLEIA
                     DDGGFEAASLAEPGMGVLGMRERVQALGGRFALTPRVPRGVLVSAEIPLHGGERGAP"
     gene            1849..2487
                     /locus_tag="PKX01_17385"
     CDS             1849..2487
                     /locus_tag="PKX01_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ77962.1"
                     /translation="MSEILKLVLVDDHAVVREGYRRLLERRANLRIVGEAGTAAEAYR
                     IVREVTPDVTILDLSLPDMGGIELIRRLVARDKDARILAFSMHQEPVFAAQALRAGAL
                     GYVTKSCAPDLLIQAVVQVAARQRVMSPDIAPELAFALLEGEDSLARDLAPREFEILR
                     LVLSGRDMKDIGDAMHLSLKTVQNTYYQTKAKLGVKSDIELMRLAMRRKWVE"
BASE COUNT          361 a          777 c          954 g          398 t
ORIGIN      
        1 gcatcgtcgc aggggggtga agggcgtgcc gggccgcggg catcccggcg ggggcggggc
       61 gtggggcgcg ccagggcctt cgggcggcct gggggtgaga tcgtccgcgc acccttgcgt
      121 gaaggattca cagggctgcg aatccttcac gcaggcgtgt gaatccttct cgcagccttg
      181 cgaatccttc tcacacgtct gtgaatcctt cccacagcct tgcgaatcct tcacacaggg
      241 ctgtgaatcc ttctcgcagc cttgcgaatc cttcgcgcag gcgtgcgaat ccttctcacg
      301 ggggtgtgaa tccttcgcgc gggctgggaa tcgtcgggac tgtcccggtg gtgctggggc
      361 gggcgccatg cagcattcag cccggtctgc gccctggcac ctgcgcgggt gttgtgggtg
      421 cgacggccca atgggcctgc ctgtcgccgt ggcgaacgat ctgccggtac catggccgtc
      481 cgcgcccttc cgcccatcgt ccatggacct gaaacgcagt ctcctcacgc gcatttcgct
      541 ctttgcgctg ctgatcttca tcgtcgcggt ggcgggggtg ctggcgcaga cccgcgcggc
      601 ggcgcgggcg cacatcgcgc gccacggtcg caccatccag cagctggtgg cggacgaggt
      661 ggcgcggtcg tcggatgcct atcaccgcaa catcgaggac ctggatctgt ccgggctgcg
      721 ggccatcggg gagctgattc acttctgcgt ggccatcgag aacctggagg cgcggccggt
      781 gacggggcgc tgcttttcgg atgcgggcga ggcgccggcg ctgctgcgtg gggcgatggc
      841 gcggctgatc ggcgacgatg tgatctaccg cggcgggctg ggctactacc cgggcatcaa
      901 gatgggggag atgacggtgc ggccggacgt ggacagcgcc gccgccgacg cgtggcggca
      961 gatccggctg atcctggcga tcacggtggg catcctgttc ctcaattttc tggtgtatcg
     1021 cccggtgcgg cgggcgctgc ggcccaccga cgcgatcctg gcggtgctgg ggcgcatgga
     1081 ggcgggggat ctgacggcgc gcatgccgtc cttcgaactg atcgaactga accgcatcgg
     1141 gcgggtgttc aacctgatgg cggagcgtct gcagcagacc gtgggtgaac accagcgtct
     1201 ggcgggccat gtgctgtcgg tgcgggagga ggagcgccgc cacctggcgc gggagctcca
     1261 cgacgagttg ggccagtgtc tgacctccat caacgcggag gcggcctacg cccacgagcg
     1321 ggcgcgggac gatctgccgg acttgcagcc gtgcaccgag gccatcgtgc gcctgacggg
     1381 gcagatgatg ggggcgttgc agcagatcct gcgggcgctg cggcccatcg ggctggagga
     1441 gttcggcctg cgggtgtgcc tggaacagtt gatcgagggc tggaaccggc gcagccgggg
     1501 gcgctgcagc taccggctgg actgggacgg cgacggggcc ggctggccgg atcgcctcaa
     1561 cgtgagcctc ttccgcatcg tgcaggaaag cctcaccaac gccacccgcc acggcgcggc
     1621 gagcgaggtg acggtgcgcc tgacccagac ggacgcgacg gtgcacctgg agatcgccga
     1681 cgacggcggc ttcgaggcgg ccagcctggc ggagcccgga atgggggtgc tgggcatgcg
     1741 cgaacgggtg caggccctgg gcgggcgctt tgcgctgacg ccgcgggtgc cgcggggcgt
     1801 gttggtgtcg gcggagattc cgctgcatgg cggcgagcgg ggcgcgccat gagcgagatc
     1861 ctgaaactgg tgctggtgga tgaccacgcg gtggtgcggg aagggtatcg ccgcctgctg
     1921 gaacgtcgcg ccaacctgcg catcgtgggc gaggcgggca cggcggcgga ggcctaccgc
     1981 atcgtccggg aggtgacgcc ggatgtgacc atcctggacc tttcgctccc ggacatgggc
     2041 ggcatcgagc tgatccgccg cctggtggcg cgggacaagg acgcccgcat cctggccttc
     2101 agcatgcacc aggaacccgt cttcgccgcc caggccctgc gcgccggcgc tctgggctac
     2161 gtgaccaaga gctgcgcccc ggacctgctg atccaggccg tggtgcaggt ggcggcacgc
     2221 cagcgggtga tgagcccgga catcgccccg gagctggcct ttgcgctgct ggaaggggaa
     2281 gacagcctgg cccgcgacct ggccccgcgg gaattcgaaa tcctccgcct ggtgctgagc
     2341 ggccgcgaca tgaaggacat cggcgacgcg atgcacctga gcctgaagac ggtgcagaac
     2401 acctactacc agaccaaggc caagctgggg gtgaagagcg acatcgagct gatgcgcctg
     2461 gcgatgcgga ggaagtgggt ggagtagggg
//
LOCUS       DAOQPA010000241         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_35067_length_2479_cov_12.290017, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000241 DAOQPA010000000
VERSION     DAOQPA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35067_length_2479_cov_12.290017"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..960
                     /locus_tag="PKX01_17390"
     CDS             115..960
                     /locus_tag="PKX01_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ77963.1"
                     /translation="MTPTRRPFLKALAALGLAASLPARADEVLDAARSRGRLRIALYD
                     KFPPYSEGEKGIDAELGQALAARLGLTPQLIPFKADDDMADDLRNMVWKGHYLRGEPA
                     DVMLHVPVDRILADANDKVRIFGAYHLETIALAREPSRVPAPGGSAAVSLEVFTREKI
                     GVEGSTLADSFLLGVLRGRLRDNVVHFRSVREAVAALKDGQLAAVLAPRGELDAALGT
                     APGIVVEDARYPELSIKQWPVGMAVKADAPDLQAALTRALAELQADGTVAAIFRKHGV
                     RQVNP"
     gene            1159..1701
                     /locus_tag="PKX01_17395"
     CDS             1159..1701
                     /locus_tag="PKX01_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77964.1"
                     /translation="MNLRRALLLTALAHATLPLRALANPNAAAADTIGRDLRKLFLST
                     RAADLGLRPDSGTPPVFGAAFDWPVGTQFATIAALTDGSASLYTTGTYNILGGQAHAP
                     VRAAAQQFVQTAAQHLGSLAPLNNHPYPATNRARLFLLTFDGLRGIELTVDDLRPGSG
                     RYAQAFANAQTVLEALRKTV"
     gene            1884..>2479
                     /locus_tag="PKX01_17400"
     CDS             1884..>2479
                     /locus_tag="PKX01_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77965.1"
                     /translation="MWFHKLRPVAEPAPDEPAAAPTGIEDAEPAASSFEQIRPAARLA
                     LLAGLALWSLRLASVDYTDADAVNTFMHLILLPLHEAGHVFLRPFGEFMTVLGGSLFQ
                     VALPLGIGAAFLLRQRDPFGAAVCLWWAGASLVDLAPYVWDALHPQLMLLNGHTGEAG
                     GHDWIYLLDHFGARPRAHRWGRVCHHLGVLTMLAGLAWG"
BASE COUNT          356 a         1070 c          689 g          364 t
ORIGIN      
        1 cttgatgcag ccgtggagcg cccgggccga acagccccgg ccgacctccc ccggccgggg
       61 ccgcagcggc gccggcgccc tgccccatcc ctcgcccgcc ccccggagac cgtcatgacc
      121 ccgacccgcc gccccttcct caaggccctc gccgccctcg gccttgccgc cagcctgccc
      181 gcccgcgccg acgaggtcct cgacgccgcc cgcagccgcg gccgcctgcg catcgccctc
      241 tacgacaaat tcccgcccta ctccgaaggc gaaaagggca tcgacgccga gctcggccag
      301 gccctcgccg cccgcctcgg cctgacgccc cagctcatcc ccttcaaggc cgacgacgac
      361 atggccgacg acctgcgcaa catggtctgg aaaggccact acctgcgcgg cgaacctgcc
      421 gacgtcatgc tgcacgtccc ggtggaccgc atcctcgccg acgccaacga caaggtccgc
      481 atcttcggcg cctaccacct ggaaaccatc gccctcgccc gcgaaccctc ccgcgtgccc
      541 gcccccggcg gctccgccgc cgtctccctg gaagtcttca cccgcgaaaa gatcggcgtc
      601 gaaggcagca ccctcgccga ctccttcctg ctcggcgtcc tgcgcggccg cctgcgcgac
      661 aacgtcgtcc acttccgcag cgtccgcgaa gccgtcgccg ccctcaagga cggccagctc
      721 gccgccgtcc tcgccccccg cggcgaactc gacgccgccc tcggcaccgc ccccggcatc
      781 gtcgtcgaag acgcccgcta ccccgaactc agcatcaagc aatggcccgt cggcatggcc
      841 gtcaaggccg acgcccccga cctgcaagcc gccctcaccc gcgccctcgc cgaactccag
      901 gccgacggca cggtcgccgc catcttcagg aaacacgggg tgcggcaggt caatccgtga
      961 tgctgccgag acgccccgtc ccgccatccc cacccccgca tcgggtccag tgtggccgtc
     1021 cggcctgcga ggaaatgact gccgggtgtt cacctcaccg agtccccacg cacctgcccc
     1081 cgtgtcactg gcaatctcta catcaaagcg cccgtagcgc tatcatcatg ccaataccat
     1141 cccgctcagg tatcgatgat gaatctacga cgcgctctcc tcctcacggc cctggctcac
     1201 gccacactac ccctcagggc ccttgccaac ccaaatgccg ccgcggccga caccatcggg
     1261 cgtgacttgc gcaagctgtt cctgtcaacc cgcgccgccg acctcggact gcgtcccgac
     1321 tcagggacgc cccccgtctt cggcgcggcc ttcgactggc ctgtcggcac ccagttcgcc
     1381 acgattgccg ccctcacgga cggctccgca agcctctaca ccaccggcac ctacaatatc
     1441 ctcggcggtc aggcacatgc tccggtcaga gccgccgccc aacagttcgt acaaacggca
     1501 gcccagcatc tgggcagcct tgcgccactg aacaaccatc cttatcccgc caccaatcga
     1561 gcccgcctct ttcttctcac cttcgacgga ctgcgtggca ttgaactcac ggtggacgac
     1621 ctccggccgg gttcgggtcg atacgctcaa gccttcgcca acgcccagac ggttctcgaa
     1681 gccctgcgaa agaccgtctg acacccggcg cagcctcacg gacgacgccc cgcccgagcc
     1741 ccccgaatcc gcgccactcc gctaccctct ccggcatgcc gtcgcctgcc ccacccccgc
     1801 cggattccgt cgccgagtgt ccccgctgcg gcgccggctt tgcgccgccc gtgccgctgc
     1861 agtcggcatg cccccgctgc ggcgtgtggt tccacaagct gcggccggtg gcggagccgg
     1921 cgccggacga gcccgccgca gcgccgaccg gcattgaaga cgccgagccc gccgcgtcct
     1981 cattcgagca gatccgccct gccgcgcgcc tggctctgct cgccggcctg gcgctctgga
     2041 gcctgcgcct tgcctcggtg gattacaccg acgccgacgc ggtgaacacc ttcatgcacc
     2101 tcatcctgct gcccctgcac gaggccggcc atgtattcct gcggcccttt ggcgagttca
     2161 tgaccgtcct cggcggcagc ctcttccagg tggccctgcc cctgggcatc ggcgccgcct
     2221 tcctgctgcg ccagcgcgac cccttcggcg ccgccgtgtg cctgtggtgg gccggcgcca
     2281 gcctggtgga cctcgcgccc tacgtgtggg acgccctgca cccccagctc atgctcctca
     2341 acggccacac gggcgaagcc ggcggccacg actggatcta cctgctggat cacttcggcg
     2401 cccgcccccg cgcccaccgc tggggccgcg tctgccatca cctgggcgtg ctgaccatgc
     2461 tcgccgggct ggcctgggg
//
LOCUS       DAOQPA010000242         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_35447_length_2464_cov_11.896222, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000242 DAOQPA010000000
VERSION     DAOQPA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35447_length_2464_cov_11.896222"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1024)
                     /locus_tag="PKX01_17405"
     CDS             complement(380..1024)
                     /locus_tag="PKX01_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HMZ77966.1"
                     /translation="MSAPLNAAPQAAPAPRPPLEFWFDFGSNYSYLSALRIEAAAARL
                     GVPVRWQPFLLGPIFRSFGWETSPFVLQKAKGAYVWRDMARACRKAGLPWQQPSVFPR
                     AALLAHRVALLGADEAWIGAFCRRVFSLNFAEDRDLEAPETVAGVLTELGLAPAALLA
                     AATTDANRQKLRQHTEAAAARGLFGAPSFFVGEELFWGNDRLDDALAAWVAQGG"
     gene            complement(1021..1905)
                     /locus_tag="PKX01_17410"
     CDS             complement(1021..1905)
                     /locus_tag="PKX01_17410"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HMZ77967.1"
                     /translation="MTALAVPRTRLRPVEPAHPAGVVAGLNYLAPTAERPVSYAFEPP
                     PGVPWENCAYALRPMPIADGRLAAPRPGIDREGFELWDAPSAVSDFADEHQIRNIYYP
                     EAMELALQVTGAQRAYVFDHLVRRRERDRAALSFGRRDLADGRPAVNGRIHNDYTEAS
                     GRARLGRVLEDPAAAARVTRFAIVNIWRSIRGPVQDTPLAVCDARSVNAADLVESEVR
                     YPRRTGEIYHLLHSPRHRWWYFARMDRHEALVFKQYDSQLGGVARFVPHTAFDLPDIP
                     ADAPLRESIELRCLVVFP"
BASE COUNT          357 a          855 c          938 g          314 t
ORIGIN      
        1 gcggaggcgt cggccggctg aggcgcagga cgcggcgcgg cgcggcggtc gtccacgggc
       61 tgtcccgccc ggcccgatcc acccgcttgc cccgtgcgcc ggtgacaggg gccgttgagt
      121 cccgtccgcc catcggggcg aaggccccat ggacgcaccc gacccgctcc gagacggcgc
      181 tgcggccgac cgggcgtgaa gggcccccag cgccgccctg cccgcgcccc gcgtcgagac
      241 cgccgccggt ggcggggtga tccgccggcc tgccgggatt ggccccccac cggcgcgtgt
      301 ccttcccgca cccggcacgg gggcaccgac cggccctccg gtggcccgcc gcgccgcccg
      361 cgggtcgccc cgctgggcct cagccgccct gcgccaccca ggccgccagc gcgtcgtcca
      421 ggcggtcgtt gccccagaac agctcctcgc ccacgaagaa gctgggggcg ccgaagaggc
      481 cccgcgcggc cgccgcctcg gtatgctggc gcagcttctg gcggttggcg tcggtggtgg
      541 cggcggcgag gagcgccgct ggggccaggc ccagctccgt gaggacgccg gcgacggtct
      601 ccggcgcttc caggtcgcga tcctcggcga agttgaggct gaacacgcgc cggcagaagg
      661 cgccgatcca cgcttcgtcc gcgccgagca gcgcgacccg gtgggccagc agggcggcgc
      721 gcgggaacac ggacggctgc tgccacggca ggccggcctt gcggcaggcc cgcgccatgt
      781 cgcgccacac gtaggcgccc ttggccttct gcagcacgaa gggcgaggtc tcccagccga
      841 agctgcggaa gatcggcccc agcaggaagg gctgccagcg cacgggcacc cccaggcgcg
      901 ccgcggcggc ctcgatgcgc agggcgctga ggtagctgta gttgctgccg aagtcgaacc
      961 agaactccag gggcggacgg ggggccggtg cggcctgcgg cgcggcgttg agcggcgcgc
     1021 tcacgggaac accaccaggc agcgcagctc gatgctctcc cgcaggggcg cgtcggcggg
     1081 gatgtcgggc agatcgaagg ccgtgtgcgg cacaaagcgc gccacgccgc ccagctggga
     1141 gtcgtattgc ttgaacacca gggcctcgtg acggtccatg cgcgcgaaat accaccagcg
     1201 atgccgcggc gagtgcagca ggtgatagat ctcgcccgtg cggcggggat agcgcacctc
     1261 actctccacc agatccgccg cattcacgct gcgcgcgtcg cagaccgcca gcggcgtgtc
     1321 ctgcaccggc ccgcggatcg agcgccagat attgacgatg gcaaagcgcg tgacccgcgc
     1381 cgccgcggcc ggatcctcca gcacccggcc cagccgtgcg cggcccgagg cctcggtgta
     1441 gtcgttgtgg atgcgtccat tcaccgccgg gcggccatcc gccaggtcgc gccggccaaa
     1501 ggacagggcc gcgcggtccc gttcccggcg ccgcaccaga tggtcgaaca cgtaggcccg
     1561 ctgcgccccc gtcacctgca gggccagctc catcgcctcg gggtaataga tgttgcggat
     1621 ctggtgctcg tccgcaaagt cactcaccgc gctcggcgca tcccacaact cgaagccctc
     1681 ccggtcgatg cccggccgcg gcgccgccag ccgcccgtcg gcaatcggca tcggccgcag
     1741 tgcgtaggcg caattctccc agggcacgcc gggcggcggc tcgaaggcat agctcacggg
     1801 gcgctccgcc gtgggcgcca ggtaattcag acccgccacc accccagccg gatgggccgg
     1861 ctcgacggga cggaggcgtg tacgcggaac agcgagggca gtcatgggct tctcctcttg
     1921 ggatgactga aggacttcgg tcggggcgac cgccgacacc cgccggcggc ctgctgccga
     1981 ttgaagccgt ccacgaaacg gcgctcaagc gagttgtggg acgctcccat gcgcgggagg
     2041 aatgtcggag ggtgagcgtg gcaggcggcc ggggcggagc gcctggttcg ccgcgcggtg
     2101 ggcttcattc gcgtcgcgga atgcttcgtt gcgccggcgg ggaagcacgc gcaggcggcg
     2161 gaatgcttcg cgcggggcgc ggagaaggat gttgggagcg cgtagtgcct tgttggcgtc
     2221 gcggggaagg actacggggg cgcggggaag gactccgcga cggtggggaa ggaatccgcg
     2281 ggcgcgggga agctcaacga tagcggcggt tgaaatgtcg gggacgcgga ttaagggttt
     2341 tggctatcgt ggaaggactc cgcgacggtg gggaaggact acgcgggcgc ggggaaggac
     2401 tacgcaggtg cggggaagct cattgatagt tgcgattcga atgtcgggga cgcggattga
     2461 gggt
//
LOCUS       DAOQPA010000243         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_36012_length_2442_cov_18.549225, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000243 DAOQPA010000000
VERSION     DAOQPA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36012_length_2442_cov_18.549225"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKX01_17415"
     CDS             <1..569
                     /locus_tag="PKX01_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ77968.1"
                     /translation="DTSFIGNAAANALTTLFTEYATLQALGARHFVIGNVPDLGLTPA
                     VRDLAALFGNPAIMSIGTAASQAFNNGLMANLFAYTAPGTSTTVFDSYGLLHDMTANP
                     AAYGLTNVTSGCYDGQPDGTPSPGASTVSECANPDGYIYFDSEHPSARAHQIGGDLLY
                     VQVVPEPAAMALSVTALGLMGLMRRRRV"
     gene            789..>2442
                     /locus_tag="PKX01_17420"
     CDS             789..>2442
                     /locus_tag="PKX01_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HMZ77969.1"
                     /translation="MSQRLREIPYNYTSFSDREIVIRLLGSDAWDTLDALRSERVTGR
                     SARMLYEVLGDMWVVQRNPYLQDDLLANRDRRGALIEALRHRLREVDKRRDETHSDDP
                     DRAHKVARLVSAAYGAVDRFEQFFDETGALRRDVLRALARHTRKDNICFDGHARVSHV
                     TDATDWRVEFPFVVLYPDTEDEMAPLVKSCIELGLTIIPRGGGTGYTGGAVPLDARSV
                     VINTEKLISLGPVEEIVLPGHDTPYATVRTGAGVVTERVSEAASAAGRVFAVDPTSAS
                     ASCIGGNIAMNAGGKKAVLWGTALDNLAWWKMVTPDGNWLEVERLEHNFGKIHEQETV
                     RFRLRRFDARDYAPLSEEVLVMPGAACRKVGLGKDVTDKFLGGVPGVQKEGTDGLIVA
                     ARWVLHKMPPVTRTVCLEFFGQVREAVPAIVEITDYFKPGGAGHAAGVQLAGLEHLDE
                     RYVKAVGYATKAKRHGRPKMVLIGDIVGHDETAVMGAASDVIRMTNTRGAEGFIAVSP
                     EQRKKFWLDRSRTAAISRHTNAFKVNEDVVIPLPRMGDYCDGI"
BASE COUNT          389 a          878 c          785 g          390 t
ORIGIN      
        1 tcgatacgtc cttcatcggc aatgcggcgg cgaatgccct gaccacgctg ttcactgagt
       61 acgccacgct gcaggccctg ggggcccgac acttcgtgat cggcaacgtt cccgacctgg
      121 gcttgacgcc ggccgtgcgc gatctggcgg cgctgtttgg caaccctgcc atcatgagca
      181 tcgggacggc tgcgagccag gcgttcaaca acgggctgat ggcgaacctg ttcgcctaca
      241 cggcgcctgg gacgagcacg accgtctttg attcctacgg cctgctccat gacatgaccg
      301 ccaacccggc ggcctacggt ctcaccaatg tgacctcggg ctgctacgac ggtcagcccg
      361 acggcacgcc ttcgcccgga gcgtccacgg tgagcgagtg cgccaacccc gacggctaca
      421 tctacttcga ttccgagcac cccagcgcgc gggcgcacca gatcgggggc gatctgctct
      481 acgtccaggt ggtgccggag ccggccgcga tggcgctgtc cgtcaccgcc ctcggcctga
      541 tgggcctgat gcgccgccgt cgcgtctgat tcccttccag gcgccgtgca gacccgccct
      601 cgtggcgggt tttttcatgg tgcgcggtcg cttgagcggg ggcacaactt tgtcgcggcg
      661 acgcggtcca ctcccccggg gacaccgtcc tcccatgccc ctgagtaccg taagggcttg
      721 ttttcctgta cagatacccg tcgtccgcta aaatgtcggg ttcttcgccg ctgaatccac
      781 gtactgcaat gagccaacgc ctgcgcgaaa tcccctacaa ctacacctcg ttctcggatc
      841 gcgagatcgt catccgcctg ctcggcagcg atgcctggga caccctcgac gccctgcgca
      901 gcgagcgggt caccggccgt tccgcgcgga tgctctacga ggtgctgggc gacatgtggg
      961 tggtccagcg caacccctat ctgcaggacg acctgctcgc caaccgtgac cggcgcggtg
     1021 ccctcatcga agccctgcgc caccgcctgc gcgaggtgga caagcgccgc gacgagaccc
     1081 attccgacga tcccgatcgc gcccacaagg tggcgcgcct ggtgtcggcg gcctatggag
     1141 cggtggatcg cttcgagcag ttcttcgacg agaccggtgc gctgcgccgg gacgtgctgc
     1201 gggccctggc ccgtcacacc cgcaaggaca acatctgctt cgacggtcac gcccgcgtct
     1261 cccacgtcac cgacgccacc gactggcgcg tggagttccc cttcgtggtc ctctacccgg
     1321 acaccgagga cgagatggcg ccgctggtga agagctgcat cgagctgggc ctgaccatca
     1381 tcccccgcgg cggcggcacc ggctacaccg gcggcgccgt gcccctggat gcccgctcgg
     1441 tggtcatcaa caccgaaaag ctgatcagcc tcggccccgt cgaggagatc gtcctgcccg
     1501 gccacgacac gccctacgcc accgtccgca ccggcgccgg cgtggtcacc gagcgggtct
     1561 ctgaagctgc gtcggcggcc ggccgggtgt ttgccgtgga cccgacctcc gccagcgcgt
     1621 cctgcatcgg cggcaacatc gccatgaacg ccggcggcaa gaaggccgtg ctgtggggca
     1681 ccgcgctcga taacctggcc tggtggaaga tggtcacgcc ggacggcaac tggctggagg
     1741 tggagcgcct cgagcacaac ttcggcaaga tccacgaaca ggagaccgtg cgcttccgcc
     1801 tgcggcgctt cgacgcccgg gattacgcgc ccctctccga agaggtgctg gtcatgcccg
     1861 gcgccgcctg ccgcaaggtg ggcctgggca aggacgtcac cgacaaattc ctcggcggcg
     1921 tgcccggcgt gcagaaggaa ggtactgacg gcctcatcgt ggcggcgcgc tgggtgctcc
     1981 acaagatgcc gccggtgacc cgcaccgtgt gcctggaatt cttcggccag gtgcgcgagg
     2041 ccgtgcccgc catcgtcgag atcaccgact acttcaagcc gggcggcgcc ggccacgccg
     2101 cgggcgtgca gctcgccggc ctggagcacc tggacgagcg ctacgtgaag gcggtgggtt
     2161 acgccaccaa ggccaagcgc catggtcgcc cgaagatggt gctgatcggc gacatcgtcg
     2221 gccacgacga gaccgcggtg atgggcgccg cctccgacgt gatccgcatg accaacaccc
     2281 gcggtgccga aggcttcatc gccgtcagcc ccgagcagcg caagaagttc tggctcgacc
     2341 gctcccgcac cgccgccatc tcgcgccaca ccaacgcctt caaggtaaac gaggacgtgg
     2401 tgatcccgct gccgcgcatg ggcgactact gcgacggcat cg
//
LOCUS       DAOQPA010000244         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_36574_length_2422_cov_18.952683, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000244 DAOQPA010000000
VERSION     DAOQPA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36574_length_2422_cov_18.952683"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1006
                     /locus_tag="PKX01_17425"
     CDS             320..1006
                     /locus_tag="PKX01_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77970.1"
                     /translation="MANNYYEATGVLVLDRVTPVIQALFGAFALDESHPGNGQAYIAQ
                     IAETTNPQWPDVLDGLEDLATQLGIPMPDDEGLSIPPLLELLAVHFGADEDEELGNLI
                     ERHSFEDTADLDALFLIATRFDDGHHLTAIQFEGCWYCSKPRLFEFGGNGCYLSREVR
                     VISSSSQALQLGDQLRKAIVAADIEEASALIALETINLLAGVSDEPFRMNLRRRVAER
                     LAQTPTISVT"
     gene            1090..1821
                     /locus_tag="PKX01_17430"
     CDS             1090..1821
                     /locus_tag="PKX01_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77971.1"
                     /translation="MSQNPNPFLRGYWNLKIVRTLSISHEDGSPHVWRNIHQSQEHLS
                     DEELVSASCIVTSDFAVVSNGPDSVSAEVLAERDAGEGAYGVVGAVVYAIHGDDFDGL
                     PVHIGDTYSAEAAREVVQRLSFETGYYSRCWEISSAHISHETGQYLANLADLATPEAF
                     LFIAFRVPYSPAIGVKLISTPWTGQHLQDVEEISAEQLRQEHCSKGMPDDLAQILALA
                     GQADVRILILDADAPVLPGLPLAGE"
     gene            1918..2196
                     /locus_tag="PKX01_17435"
     CDS             1918..2196
                     /locus_tag="PKX01_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HMZ77972.1"
                     /translation="MFPDLISPVSDFEHQLGACFNAMSQDDAIGQILVVERMSGMLHM
                     RHIASADLMDTDVDDYEMVIFDGGNTSGDTWKHVFFPRQREHYFVYQA"
BASE COUNT          440 a          828 c          697 g          457 t
ORIGIN      
        1 ggtcagcttc tacaacgcgc gcgagggcgg tgccatgctc aagcgctgcc acttcaacgg
       61 gttgtcggac ttcgacggcc tcgatctgcc tcgtcgccag gtgatcgccg acctcctgct
      121 gaactacagc ggttggtgag ccggtccccg gcacaccttc atcactgcgt tcacgcgacc
      181 ccatgaggga catgcgcctc gcccgaggcc atgtccctcg cgtttctccc cgcccagtgc
      241 catccggcat caggcggttc ccctgcggat gccatcgtcc gcaagggctg gctccgttca
      301 ttcatcgaaa ggagccttca tggccaacaa ttactacgaa gccaccggcg ttctcgtgct
      361 cgaccgtgtg acgcccgtca tccaggcgct gttcggcgcc ttcgcgctgg acgagagcca
      421 tcccggcaac gggcaggcct acatcgccca gatcgccgag accaccaatc cgcaatggcc
      481 ggacgtgctc gatggcctgg aagacctggc cacgcaactc ggcattccga tgccggacga
      541 cgaagggctt tcgatcccgc cgctgctcga actgctcgcc gtgcacttcg gtgccgatga
      601 ggacgaagag ctggggaact tgatcgagcg tcattcgttc gaggacaccg ccgatctcga
      661 cgcattgttt ctgatcgcca cccgattcga tgacggccac cacctgaccg ccatccagtt
      721 cgagggatgc tggtattgca gcaagccgcg gctgttcgag ttcggcggca acggctgcta
      781 cctgagccgc gaggtccgcg tcatcagttc ctcctcgcag gccctccagc tcggcgacca
      841 actgcgcaag gccattgtgg ccgccgacat cgaagaggcc tcggccctga ttgcgctgga
      901 gaccatcaat ctgctggcgg gcgtcagcga cgagccattc cgcatgaatt tgcgccgacg
      961 cgtcgccgag cgattggccc aaacgccaac gatcagcgtc acctgacgct gtttcctttc
     1021 tttccaccca ccggggtcaa ttcccggtgg cggggattgg ctccatcatt attctgttgg
     1081 agaaatccca tgtcccagaa tcccaatccc tttctacgcg gctactggaa tctgaaaatc
     1141 gtccgcacgc tgtccatcag ccacgaggac ggaagcccgc atgtctggcg aaacatccac
     1201 cagagccaag aacacctctc cgatgaggag ctggtttcag catcctgcat cgtcaccagc
     1261 gatttcgccg tagtcagcaa tggcccagac tccgtgagtg ccgaggtgct ggccgaacgc
     1321 gatgccggtg aaggcgccta cggcgtggtg ggtgccgtgg tgtacgcgat ccacggcgac
     1381 gacttcgacg gcctccccgt ccacattggc gacacttatt cggccgaggc cgcacgggaa
     1441 gtcgtgcagc ggttgagctt cgagaccggc tactacagcc ggtgctggga aatcagcagc
     1501 gcgcacatca gccacgaaac cggccagtac ctggccaacc tggctgacct cgcgacgccg
     1561 gaagcctttt tgttcattgc gttccgggtt ccctacagcc cggcgatcgg cgtcaagctg
     1621 atttccacgc cctggacggg ccagcacctg caggacgtcg aggagatctc cgccgagcag
     1681 cttcggcagg agcactgcag caagggcatg ccggacgacc tggcacagat ccttgcactg
     1741 gccggccagg ccgatgtgcg catcctcatt ctcgatgccg acgcacccgt gttgccgggc
     1801 ctgccgttgg ccggcgaata actgccagac acatccccag cctacctttt cctcaccgca
     1861 tgccggcccg tctcccgcca gggagccggg ccggttcact ttcataggag cgatcccatg
     1921 ttccccgatc tcatctcgcc cgtatcggac ttcgagcacc agcttggagc ctgcttcaat
     1981 gccatgagcc aggacgatgc catcggccag atcctggtag tcgagcgcat gagcggcatg
     2041 ctgcacatgc gccatatcgc cagcgccgac ctgatggaca ccgacgtgga cgactacgaa
     2101 atggtcatct tcgacggtgg caacacaagc ggggacacgt ggaagcacgt gttctttccg
     2161 cgtcagcgcg agcactactt cgtgtaccaa gcctgacccc tccggcccct ttcgaggggc
     2221 tttttcttgt acgcagcaca ggaatgcggg catgttgcgg tgccgtttcc tgcgggcagg
     2281 ccgaccaccc cggggccatg cttaccccat gtccgtcggc gctgccgaca catgcccagg
     2341 cagtcgagac cttcgaggct gcaagcacgg gaaaccgtgc tggtcgtttc ttcctacgga
     2401 cctaccgcgt ccatccacgc ca
//
LOCUS       DAOQPA010000245         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_36743_length_2416_cov_12.988988, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000245 DAOQPA010000000
VERSION     DAOQPA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36743_length_2416_cov_12.988988"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1074)
                     /locus_tag="PKX01_17440"
     CDS             complement(202..1074)
                     /locus_tag="PKX01_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ77973.1"
                     /translation="MRRPSATLHVPSPVLGACVMAGVERDTRGCTLTDRERFNFYPAS
                     PMASISWIFEGALHLMEDDGGAGGASTLTGLGPALPRLILAGPHTRPTASWSPGSVHA
                     LMVGIYPEAMARLLGRPVAAWLNRTVPLADVAPPALLDACNAVFGAPDAPFAKLETTL
                     RALWNLPAQGSPIPYLGDWVRGLATRATHSGAGCSLRQWQRRIRAWTGQSQRDLEVFV
                     RIEEAFVRRIAAGPAPNLAMIAAASGYADQSHMGRDIKRVTGVAPARFGERMAADESF
                     WYYRLIADAIARQG"
     gene            1121..2215
                     /locus_tag="PKX01_17445"
     CDS             1121..2215
                     /locus_tag="PKX01_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ77974.1"
                     /translation="MAAVMFRTPIMPKTHFHGRLSHRLRSLATAACIATSLAARRLCG
                     RRRVASWSFLFEYGTLYIRAQFNHAFRLKDDIAASRAYFDSLYSVFETYPDVTVRETG
                     PGEPRGHWFLPDNRRHDITLLHFHGGGYTFYAGVSHHFARILAHSLGVAVFAPDYRLT
                     PEHPHPAQLEDGLAAYRHLLATGHTPERIVLCGDSAGGHLALMTLAHLAGAGLPRPRL
                     TLGISPWTDIGRRGASQFGNDPYDLVQGYMTLQFADWLKGGQDWRTDDLSPIHHDYRG
                     LGPLYLQAGGREILVDMIRDFAASVAGRGGQVRLDVWPDMNHEFHGYGDHLPESREAL
                     ARLERAIAWALQPDTPFVADTLTETDTLTS"
BASE COUNT          397 a          904 c          733 g          382 t
ORIGIN      
        1 ccgaccattg attcaagcaa aaagccaacc tttcggggtt ggctttttgc tttgtggccg
       61 gctccacctt cttcccacag ccgtcgttcg cagcgccgtg tctgctggca gctcactcca
      121 aacgccaccc gggtcctgca atcgacgtcg gggacgaggg cacagcgcga tgtacgtccc
      181 tgcaggtccg agcggtaccg gtcagccttg tcgcgcgatt gcgtcggcaa tcaaccggta
      241 ataccagaac gactcgtccg cggccatccg ttctccaaac cgggccggcg ccacaccagt
      301 gacccgcttg atgtcccggc ccatatggga ttggtcggcg taaccggacg ctgcggcgat
      361 catcgccaga ttgggtgccg gcccggcggc gatgcgccgc acaaacgcct cctcgatgcg
      421 gacaaagacc tccaggtcgc gctggctctg gcccgtccag gcgcggatgc ggcgctgcca
      481 ttggcgcaga ctgcaccccg ccccggagtg cgtggcgcgc gtcgccagac cccgcaccca
      541 gtcccccaga tacgggatcg ggctgccctg ggccgggaga ttccagagcg cccgcagcgt
      601 cgtctccagt ttcgcgaacg gggcgtccgg cgccccgaat accgcgttgc aggcgtcaag
      661 cagggcgggc ggcgcgacgt ccgccaaggg aacggtccgg ttcagccagg cggccacggg
      721 ccgaccgagc agacgcgcca tggcctccgg gtagatcccc accatcagcg catgcaccga
      781 gcccggcgac cagctcgccg tcggccgcgt atgcggaccg gcaaggatca ggcgcggaag
      841 ggccggcccc agacccgtca aggtcgaagc accgccagcg cccccgtcgt cctccatcag
      901 atgcagggcg ccctcgaaaa tccacgagat cgacgccatg ggactcgccg gatagaagtt
      961 gaagcgctcc cggtcagtca gcgtgcagcc acgggtatcg cgctccaccc cggccatcac
     1021 gcaggccccc aacacgggcg aagggacatg cagggtcgcg gagggtcggc gcatgggatg
     1081 tcgtttccgt tctagcgtga cagggacggg gaccgtcagc atggcggctg tcatgttccg
     1141 gacgcccatc atgcccaaga cccattttca cggccgttta tcccacagac tgcgcagtct
     1201 tgccacggcc gcatgcatcg ccacctccct tgccgcacgc cggctctgcg gtcgccgccg
     1261 ggttgcatcc tggtccttcc tgttcgagta cggcacgctg tacatccgcg cccagttcaa
     1321 ccatgccttt cgcctgaagg acgacatcgc cgccagtcgc gcctatttcg acagcctcta
     1381 ttccgtattc gagacctatc ccgacgtcac cgtccgcgag accgggccag gcgaaccacg
     1441 aggccactgg ttcctgcccg acaaccgccg gcacgacatc acgctgctgc acttccacgg
     1501 gggcggttat accttctacg ccggcgtgtc ccatcacttc gcccgcatcc tggcgcacag
     1561 cctgggggtg gcagtcttcg ccccggatta ccgtctgacc ccggagcacc cccaccccgc
     1621 ccagctggaa gacggactgg ccgcctaccg ccatctcctt gccacgggtc acacgccgga
     1681 gcgcatcgtg ctctgcggcg attccgcagg cggtcacctg gcgctgatga ccctcgccca
     1741 tctcgccggc gccggcctgc cccggccccg cctcacgctg ggaatcagtc cctggaccga
     1801 cattggccga cggggcgcga gccagttcgg caacgacccc tacgacctcg ttcaaggcta
     1861 catgaccctg cagttcgcag actggctcaa gggcggacag gactggcgca cggacgacct
     1921 ctcaccgatc catcacgact atcgcggcct cgggcccctc tatctgcagg cgggcgggcg
     1981 cgagatcctc gtcgacatga tccgcgactt cgccgcgagc gtggccggcc ggggcggcca
     2041 ggtgcgcctg gacgtgtggc cggacatgaa tcatgaattc cacggctacg gggaccatct
     2101 ccccgaaagc agggaggccc tggcgcgcct cgagcgggcg attgcatggg cactgcagcc
     2161 cgacacgccc ttcgtcgccg ataccctcac cgagacggac accttgacct catgacaccc
     2221 cgggcgggca ggcttccgcg ccccaggatg tccgaacgct ggcgccgaca tcgcgtcatg
     2281 ggcggggcgc ccgccctcaa gtccaacgcc ccccgtccgt aaatcctgag cgtcctgccc
     2341 gttgccggtt cgacgttctc ccatcgttac ggatccgccc gacgccatgc caacttcccg
     2401 ccaccgtacg gttgcc
//
LOCUS       DAOQPA010000246         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_37280_length_2397_cov_13.379590, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000246 DAOQPA010000000
VERSION     DAOQPA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37280_length_2397_cov_13.379590"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1045
                     /locus_tag="PKX01_17450"
     CDS             323..1045
                     /locus_tag="PKX01_17450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMZ77975.1"
                     /translation="MQTPALAPLPARVSVILTCSNAAAYVGEAIESVLVQSRPCDELI
                     VVDDGSDDTSAQVIQGFGAALTCVRQESLGVAAARNRGVLQARGDIVAFLDAEDLWPA
                     DSLATRLACLDAHPGLDYAFGWVEPFFSPDIPEPVRAAIGDAGLILPGRVAGSLLVRR
                     RVFESVGLFNNSFRVGEALEWITRANAAGFRSRDVGAIVLRRRLHGTESGAHAQQAQH
                     DYQRILRTTIGRSRLTAGRAYA"
     gene            complement(1151..2167)
                     /locus_tag="PKX01_17455"
     CDS             complement(1151..2167)
                     /locus_tag="PKX01_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ77976.1"
                     /translation="MTARLKSATRRSVLSLALVLGLSSLAHADVTLLNVSYDPTRELY
                     QDYNAAFAKYWKARTNETVTIKASHGGSGKQARSVIDGLEADVVTLALAADIDALYEH
                     GKLVPADWQKRLPHNASPYTSTIVFLVRKGNPKKIRDWNDLAKPGIDVITPNPKTSGG
                     ARWNYLAAWGYALKQPGGNEASAKDFVKKIYGNVKVLDSGARGATTTFVERGIGDVLI
                     AWENEAYLAVKELGPDKFDIVTPSLSILAEPPVSVVDKVVDKRGTRKVAEEYLKYLYS
                     SEGQDIAGQNYYRPTDPKVAAKYAKQFAPVKLFTIDEVFGGWTKAQKTHFADGGVFDQ
                     ISVK"
BASE COUNT          361 a          907 c          732 g          397 t
ORIGIN      
        1 ccctggggca gctcgccgcc caacgggaag acgccaccct gatcgtcacc cgcgtcgaga
       61 cgcccctcgc ctacgatgcc gacacggacg gcctgctgtg gcagggcgat gccttcggtg
      121 cgccgcaggc acgcccgtcc gcgcggcgct gacaggcgca acacgccgcc cgcgacgccc
      181 cgtcgcgatc gtgacatttt gcatatttca ttgattgaca tgcaaaatcc gcaatccaaa
      241 aggcgtatgc tgtgcagatt ctcaatttat cgatatgcca tcgtcgcggc gacctccccg
      301 tggcgctgac ctgacggccc ccatgcagac tcccgccctt gccccgctcc ctgcccgcgt
      361 cagcgtgatc ctcacctgct cgaacgcagc cgcctacgtg ggcgaagcca tcgagagcgt
      421 cctcgtccag tcccgcccct gcgacgaact catcgtggtg gatgacggct ccgacgacac
      481 cagcgcgcag gtcatccagg gcttcggcgc cgcgctcacc tgcgtccgcc aggaatccct
      541 cggcgtcgcc gccgcccgca accggggcgt gctgcaagcc cgcggcgaca tcgtcgcctt
      601 tctcgacgcc gaagacctgt ggcccgccga cagcctcgcc acgcgcctcg cctgcctgga
      661 cgcccatccc ggcctggact acgccttcgg ctgggtcgaa cccttcttca gcccggacat
      721 tcccgagccc gtgcgcgccg ccatcggcga cgccggcttg atcctgcccg gccgcgtggc
      781 cggttccctg ctcgtgcgcc ggcgcgtctt cgagagcgtc gggctcttca acaacagctt
      841 ccgcgtcggc gaagccctcg aatggatcac ccgcgccaac gccgccggct tccgctcccg
      901 cgacgtgggc gccatcgtgc tgcgccgccg tctgcacggc accgaatccg gcgcccacgc
      961 ccagcaggct cagcacgact accagcgcat cctgcgcacc accatcggcc gcagccgcct
     1021 gactgccggc cgggcctacg cctgagccac cccgctcccc gcccgcccgt cgccccgtcg
     1081 ttcacatccc cccgtccacg cacaacggcc ggacctcccg gcccggccgt cctgccgcac
     1141 acgccgcggc ttacttcacc gagatctgat cgaacacccc gccgtcggcg aagtgggtct
     1201 tctgggcctt ggtccagccg ccaaacacct cgtcgatggt gaacagcttc accggcgcaa
     1261 attgcttggc gtacttggcc gccaccttcg gatcggtcgg ccggtagtag ttctggccgg
     1321 cgatgtcctg gccctcgctg gagtacaggt acttcaggta ttcctccgcc accttgcgcg
     1381 tgccgcgctt gtccaccacc ttgtccacca ccgacaccgg cggctcggcc aggatggaga
     1441 gggacggggt gacgatgtcg aacttgtccg gacccagctc cttcaccgcc aggtaggcct
     1501 cgttctccca ggcgatcaac acgtcgccga tgccccgctc cacaaaggtg gtggtggcac
     1561 cgcgggcgcc cgaatcgagc accttcacgt tgccatagat cttcttcacg aagtccttgg
     1621 cgctggcctc gttgccgccc ggctgcttga gggcgtagcc ccacgccgcc aggtagttcc
     1681 agcgggcgcc gccggaggtc ttgggattcg gcgtgatcac gtcgatgccc ggcttggcca
     1741 ggtcgttcca gtcgcggatc ttcttcgggt tgcccttgcg caccaggaag acgatggtcg
     1801 aggtgtaggg cgaggcgttg tggggcaggc gcttctgcca gtcggcgggc accagcttgc
     1861 cgtgttcata gagcgcgtcg atgtcggctg ccagggccag ggtgaccacg tcggcctcca
     1921 ggccgtcgat caccgagcgg gcctgcttgc cggaaccgcc gtgggaggcc ttgatggtca
     1981 cggtctcgtt ggtgcgcgcc ttccagtact tggcgaaggc ggcgttgtaa tcctggtaga
     2041 gctcacgggt cgggtcgtag gacacgttga gcagggtcac gtccgcatgg gcgaggctgg
     2101 acagccccag caccagggcg agggacagca cggaacggcg ggtggcggat ttgaggcggg
     2161 cggtcatggc agattcctgt cgacaacgaa agatggcgcc gagtctaggc agccccccac
     2221 gccggcccaa ccaagaaaaa ctgccatgct tattccgcag cgcgtgaaat ccattgcata
     2281 aaatgcccca gcccgcgccc atcgcccccg ccgcgttgcg cccgcgcgac accttcagcg
     2341 cccccacccg gcgcctgccg ccgccccgtc aagccacgct gcaccgccgc acggggc
//
LOCUS       DAOQPA010000247         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_39134_length_2335_cov_11.728947, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000247 DAOQPA010000000
VERSION     DAOQPA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39134_length_2335_cov_11.728947"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2335
                     /locus_tag="PKX01_17460"
     CDS             <1..>2335
                     /locus_tag="PKX01_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HMZ77977.1"
                     /translation="EHNLDVIAAADWLLDLGPEGGRDGGRLVAEGTPADLMTCPASHT
                     GRALADYAAQLAALQSAPRVAEAAPRYRPVAAPAIEIRHARHHNLRNISLSIPRDRFT
                     VVTGLSGSGKSTLAFDIVFGEGQRRYLESLNAYARQFAQPSARPDVDAIFGIPPTVAI
                     EQRVSRGGRKSTVGTLTEIQPFLRLLYVKLGTQYCPDCDVPVTPQSFEAIVAQLQRDF
                     AGRSVELLAPLVVNRKGLYTDLAKWARGKGFEQLRVDGDYLPTRKWPRLDRYKEHVIE
                     LPVGMVRIGPDTETALRDAVQGALDHGKGVLKALPLGEAGARPLTLSTLRACPDCGTS
                     FPEPDPRLFSYNAKHGWCPDCYGTGLRLAGKVDNPDDLDLGEADAVSDEACPSCHGAR
                     LNPVARAVRFRDLGLHELSSLAVGQVAHFFGGLALTPREADIARDLVAEIRGRLDFLQ
                     QVGLAYLALDRAAPTLSGGEAQRIRLAAQLGSNLTGVCYILDEPTIGLHPRDNRLLLD
                     TLEALRARGNTLLVVEHDEDTIRRADHVIDLGPGAGVRGGRVVAEGRLEALMAAPESA
                     TGRCLAAPLQHPLAARRPVTDTHPALVVTDATLHNLKHAAARVPLARLTVVTGVSGSG
                     KSTFARDVLHASLTAAAPVGCAAVDGSGAIGRVLEVDQTPIGKTPRSCPATYIGFWDA
                     IRKLFAETTEAKLRGWNASRFSFNAGAGRCPVCEGQGQITVEMNFLPDVKLPCEACGG
                     ARFNPETLSVRWKDRTVAEVLAMAVEDAVDFFAAHPA"
BASE COUNT          328 a          969 c          735 g          303 t
ORIGIN      
        1 tcgagcacaa cctggacgtg atcgccgccg ccgactggct cctcgacctg ggcccggaag
       61 ggggtcgcga cggcggccgc ctggtcgccg aaggcacgcc ggcagacctc atgacctgcc
      121 ccgcctccca caccggccgc gccctcgccg actacgccgc ccagctcgct gccctgcaaa
      181 gcgcaccgcg ggtggccgaa gcggcgccgc gctaccgccc ggtggcggca cccgccatcg
      241 agatccgcca cgcccgccac cacaacctgc gcaacatcag cctgtccatc ccccgcgacc
      301 gcttcaccgt ggtcaccggc ctgtccggct cgggcaagtc cacgctggcc ttcgacatcg
      361 tcttcggcga aggccagcgc cgctacctgg aatccctcaa cgcctacgcc cgccagttcg
      421 cccagccctc ggcgcggccg gacgtggacg ccatcttcgg cattccgccc accgttgcca
      481 tcgagcagcg ggtcagccgc ggcgggcgca agagcacggt tggcaccctc accgagatcc
      541 agcccttcct gcgcctgctt tacgtcaagc tgggcaccca gtactgcccg gactgcgacg
      601 tgccggtcac gccccagagc ttcgaggcca tcgtcgccca gctccagcgc gacttcgccg
      661 gccgctcggt ggaactcctc gccccgctgg tggtgaaccg caagggcctg tacaccgacc
      721 tggccaaatg ggcgcggggc aagggcttcg agcagttgcg cgtggacggt gactacctgc
      781 ccacccgcaa gtggccacgc ctcgaccgct acaaggagca cgtcatcgag ctgccggtgg
      841 gcatggtgcg catcggtccg gacaccgaaa ccgccctgcg cgacgccgtg cagggggccc
      901 tcgaccacgg caagggcgtg ctcaaggccc tgcccctggg cgaggccggc gcccggccgc
      961 tgaccctctc caccctgcgc gcctgcccgg actgcggcac cagctttccc gagcccgacc
     1021 cgcgcctgtt ctcctacaac gccaaacacg gctggtgccc ggactgctac ggcaccgggc
     1081 tgcgcctggc cggcaaggtg gacaacccgg acgacctgga cctgggcgag gcggacgccg
     1141 tcagcgacga agcctgcccc agctgccacg gcgcccgcct caacccggtg gcccgcgccg
     1201 tgcgcttccg cgacctgggc ctgcacgagc tctccagcct cgccgtcggc caggtggcgc
     1261 acttcttcgg cggcctcgcc ctgacgcccc gcgaggccga catcgcccgc gacctggtgg
     1321 ccgagatccg cggccggctc gacttcctgc aacaggtcgg cctcgcctac ctggccctcg
     1381 accgggccgc gccgaccctt tccggcggcg aagcccagcg catccgcctg gccgcccagc
     1441 tcggctccaa cctcaccggc gtgtgctaca tcctggacga acccaccatc ggcctgcacc
     1501 cccgcgacaa ccgcctgctc ctcgacaccc tggaggccct gcgcgcccgc ggcaacaccc
     1561 tgctggtggt ggagcacgac gaggacacca tccgccgcgc cgaccacgtc atcgacctgg
     1621 gccccggcgc cggcgtgcgc ggcggccggg tcgtcgccga agggcgcctg gaggcgctca
     1681 tggcggcgcc ggaatccgcc accggacgct gcctcgccgc gccactccag catcccctcg
     1741 ccgcccgccg cccggtcacc gacacgcatc ccgccctggt cgtgacggac gccaccctgc
     1801 acaacctcaa gcacgccgcg gcgcgggtgc ctctggcgcg cctcacggtg gtcaccggcg
     1861 tgtccggctc gggcaaatcc accttcgccc gcgacgtgct ccacgccagc ctcaccgccg
     1921 ccgcgccggt gggctgcgct gcggtggatg gcagcggcgc catcggccgc gtgctggagg
     1981 tggaccagac ccccatcggc aagaccccgc gctcctgccc ggccacctac atcggcttct
     2041 gggacgccat ccgcaagctc ttcgccgaga ccaccgaggc caagctgcgc ggctggaacg
     2101 cctcccgctt ctccttcaac gccggcgcgg gccgctgccc ggtgtgcgaa ggccagggcc
     2161 agatcaccgt ggagatgaac ttcctgcccg acgtgaagct gccctgcgag gcctgcggcg
     2221 gcgcccgctt caacccggaa accctcagcg tgcgctggaa ggaccggacc gtcgccgagg
     2281 tgctggcgat ggccgtggag gacgccgtgg acttcttcgc cgcccaccct gccat
//
LOCUS       DAOQPA010000248         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_41521_length_2259_cov_13.996370, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000248 DAOQPA010000000
VERSION     DAOQPA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41521_length_2259_cov_13.996370"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKX01_17465"
     CDS             <1..530
                     /locus_tag="PKX01_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ77978.1"
                     /translation="KHPRHAAVLRLAADKAGWGTPLAAGKPGERRGRGVAVHESFHSF
                     VAQVAEVTVAADGSVKVDRIVAAVDCGVAINPDNVRAQVEGSVAFALSAALHGEITLQ
                     EGRVVQSNFHDYAPLRLNEMPRVEVHIVPSAANPTGIGEPGVPPVAPAVANAIAAATG
                     TWLTRLPFNADALKA"
     gene            647..1648
                     /locus_tag="PKX01_17470"
     CDS             647..1648
                     /locus_tag="PKX01_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HMZ77979.1"
                     /translation="MDGQDLQVLAAVRRWLADGHRVALVTVARTWGSAPRPPGAWMAL
                     RDDGRVQGSVSGGCIEDDLIARMGAGEIVGRAPFVLRYGVTRDEAARFGLPCGGTLEL
                     VVEPAPDRALLATLAERLTAGRLVRRDVDLASGATRLADAHRGEAMAWDGTRLSTLHG
                     PRLRLLLIGAGQIARSLAPMAQALDYAVSVCDPREEYAREWDVPGVPWVAGMPDDAVL
                     AMNPDPHSAIVALTHDPKLDDMALLEALKSPAFYVGALGSRSNNAARKARLLRYFDLS
                     DAEVARLHGPVGIPIGSRTPPEIAVSILAGLTAVKNGVALPLAVTDAPPASAGCGVA"
     gene            1663..2235
                     /locus_tag="PKX01_17475"
     CDS             1663..2235
                     /locus_tag="PKX01_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ77980.1"
                     /translation="MTSNLVGLLLAAGRGTRFGSDKLLHPLPDGTPLAVAAARTLQAA
                     CPRCVAVLRPEQTGLAECLTREGLAVVFSPEARAGMGHSLAAGVATTPEAAGWLVTLA
                     DMPFLRVDTVQAVMQALAGGAALAAPVCAGRRGHPVGFAARWRDELQGLTGDEGARAL
                     LGRHRALIVGIETDDAGVLRDVDVREDLGG"
BASE COUNT          267 a          796 c          867 g          329 t
ORIGIN      
        1 agaagcatcc gcgccatgcc gcggtgctgc ggctggcggc ggacaaggcc ggctggggca
       61 cgcccctggc ggcgggcaag cccggcgagc ggcgcggccg tggcgtggcg gtgcatgagt
      121 ctttccattc cttcgtcgcc caggtggcgg aagtcaccgt cgcggcggac ggcagcgtga
      181 aggtggatcg gatcgtcgcc gcggtggatt gcggcgtggc gatcaacccg gacaacgtgc
      241 gcgcccaggt cgaaggttcg gtggccttcg cgctctccgc cgccctgcac ggggagatca
      301 ccctgcagga gggccgcgtg gtgcagtcca acttccatga ctacgccccc ctgcgcctca
      361 acgagatgcc gcgggtcgag gtgcacatcg tgccctcggc ggccaatccg accggcatcg
      421 gcgagcccgg cgtgccgccc gtggcgcccg cggtggccaa cgccatcgcc gcggccacgg
      481 gcacctggct gacccgtctg cccttcaatg ccgacgcgct gaaagcctag gatgatggcc
      541 cgacttcagc ttgacgacgc cgggtccgcc gtggcgagcg tgcggcacgg tagcgcgccc
      601 gcccggggcc cgactgccgt gcggggcgcg gggcaggaga tctgacatgg acggccagga
      661 tctgcaggtg ctcgcggccg tgcgccgctg gctcgccgac ggccaccgcg tcgcgctggt
      721 cacggtcgcc cgcacgtggg gctcggcgcc ccgtccgccc ggcgcctgga tggccctgcg
      781 ggacgatggc cgggtacagg gttcggtgtc cggcggctgc atcgaagatg atctgatcgc
      841 ccgcatgggg gccggcgaga ttgtcggccg tgcacccttc gtgctgcgtt acggcgtcac
      901 ccgcgatgag gcggcccgtt tcggcctgcc ctgcggcggt acgctggaac tggtggtgga
      961 accggcgcct gacagggccc tgctggcgac cctcgctgag cgtctgacgg cagggcggct
     1021 ggtgcgccgc gacgtggatc tggccagcgg cgcgacgcgc ctggccgatg ctcaccgggg
     1081 cgaggccatg gcctgggacg gcacgcgcct gtccacgctg cacggaccgc gtctgcgcct
     1141 gctcctcatc ggcgcgggcc agatcgcccg cagcctggcg cccatggccc aggccctgga
     1201 ctacgccgtc agcgtctgcg atccgcggga ggaatacgcc cgcgagtggg atgtgcccgg
     1261 cgtgccctgg gtggccggca tgcccgacga cgcggtgctg gcgatgaatc ccgaccccca
     1321 cagcgccatc gtggccctga cccacgatcc caagctggac gacatggccc tgctggaggc
     1381 gctcaagtcg ccggccttct atgtgggagc cctcggctcg cggagcaaca acgcggcccg
     1441 caaggcgcgt ctgctgcgat acttcgacct ctcggatgcg gaggtggcgc gcctccacgg
     1501 tccggtgggc attcccatcg gcagccgtac gccgccggag atcgcggtgt cgatcctggc
     1561 cggcctcacc gccgtcaaga acggcgtggc gttgccgctg gccgtcaccg acgcgccgcc
     1621 cgccagcgcc ggctgcggcg tagcctgacg tcggcaggtg cggtgacgtc gaaccttgtc
     1681 ggcctcctgc tggcggcggg gcggggcacc cgtttcggca gcgacaagct gctccacccg
     1741 ctgcccgacg gcacgcccct cgcggtggcc gctgcgcgta ccttgcaggc cgcctgcccg
     1801 cgctgcgtgg cggtgctgcg tcccgagcag accgggctgg cggaatgcct gacgcgggaa
     1861 ggcctggccg tggtgttcag cccggaggcc cgtgccggca tgggccacag ccttgcggcc
     1921 ggcgtggcga cgacgccgga ggctgccggc tggctcgtca ccctggccga catgcccttc
     1981 ctgcgggtcg atacggtgca ggccgtgatg caggcgctgg ccggcggcgc ggcgctggcg
     2041 gcgcccgtct gcgcgggtcg ccggggccat cctgtcggct tcgccgcccg ctggcgcgac
     2101 gaactgcagg ggctgacggg cgatgaaggc gcccgcgcgc tgctgggacg tcatcgggcg
     2161 ctgatcgtcg gcatcgagac ggacgatgcg ggggtgttgc gggatgtgga tgtgcgggag
     2221 gatctgggcg gctgaggggg cctcgtgccc cgtgggggg
//
LOCUS       DAOQPA010000249         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_43517_length_2202_cov_13.195156, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000249 DAOQPA010000000
VERSION     DAOQPA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43517_length_2202_cov_13.195156"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..356)
                     /locus_tag="PKX01_17480"
     CDS             complement(57..356)
                     /locus_tag="PKX01_17480"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HMZ77981.1"
                     /translation="MTTYIAFLRSGPDGACTVSFPDLPGCTVCGANAVLATALAGDEL
                     VRYLSVLLSLGEAAPPPTSEAALAQDPFRGDAALLAFDVDLEMLGIAHPLHFHTL"
     gene            complement(395..769)
                     /locus_tag="PKX01_17485"
     CDS             complement(395..769)
                     /locus_tag="PKX01_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HMZ77982.1"
                     /translation="MVDRIVATPEAVDLLNRLRARHGPRLLFLQACGCCDHSSLYCYP
                     DTEYAVAPQDVLVGHIDGVPYYQDAQQHALFRHTQILIRVVPGDRSGDMSLEGTDGVI
                     FRLESRLFSADEIAALDAPAAA"
     gene            complement(1049..>2202)
                     /locus_tag="PKX01_17490"
     CDS             complement(1049..>2202)
                     /locus_tag="PKX01_17490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ77983.1"
                     /translation="SLAGTWMQQIAMVWLAYRLSGSAFVLGMVGFASQAPILLFGAFG
                     GVVTDRLDRRRVLLATQALSMVQALLLAALAWKDAATPGHLVGLAFVLGCINALDVPA
                     RQAIAVQLVDDPDDLPNAIALNSFLMNTARFVGPALAGFVVAEVGEAVCFLLNAASYL
                     AVLMALRAIRLPAAGDRPPAPPPLQALREGWRYAMAHREIRASLRLVAAISLCAAPYV
                     VLMPLFAREEFGGGARLFGLLVGSAGAGSLVASFYLAGRADTGGLAEKVAVAAPTVGV
                     AVILFALSPGVGMAVPVLLALGGSIILTIAGSNTLIQTQVDNAFRGRVMALFSMAFLG
                     VAPLGSFTVGSAAHVFGVRPTLVVCGVLTVIAGLIYRRAVLRRKAAAAA"
BASE COUNT          380 a          827 c          755 g          240 t
ORIGIN      
        1 gcccccgcgg gcaccccgcc tgacgcgcac gccgcccgcc cgtgcccggc gcgcggctag
       61 agggtgtgga agtgcagggg atgggcgatg cccagcatct ccagatccac gtcaaaagcc
      121 agcagggccg cgtctccgcg gaacgggtcc tgggccaggg cggcttccga ggtggggggc
      181 ggtgcggctt cgccgaggct cagcagcacg ctcaggtagc gcaccagttc gtcccccgcc
      241 agcgccgtgg ccagcacggc gttggcgccg cagacggtgc agccgggcag atccggaaag
      301 ctcacggtgc atgcgccatc cgggccgctg cgcagaaagg cgatgtaggt ggtcatggtg
      361 tccgcctcgt tgaaaaggat cggcagcccg ctgttcaggc ggcggccggt gcgtccagcg
      421 cggcgatctc gtcggcgctg aacaggcgcg attccaggcg gaagatcacc ccgtcggtgc
      481 cctccagcga catgtcgccg ctgcggtcgc cgggcaccac gcggatcagg atctgggtgt
      541 ggcggaacag ggcgtgctgc tgggcgtcct ggtagtaggg cacgccgtcg atgtggccga
      601 cgagcacgtc ctggggcgcc accgcgtact ccgtgtccgg gtagcaatac agcgagctgt
      661 ggtcgcaaca gccgcaggcc tgcagaaaca gcagccgcgg gccgtgccgc gccctgagcc
      721 ggttcaaaag atccacggcc tccggcgtgg ccacgattcg atccaccatg gggggctcct
      781 ccgcttgtgg ggcgcgcggc cgggcgatgg cagagcccgc ggcgccgggg tggggacgtc
      841 ccgcgggctg ccgaacagcc cggcccgcgg gtggaggaca tcatggaagg acccgcggcg
      901 tggcacggga cataaaagcg gcaattcggg tcatcgtgag gggatgcacg ccgcggcggg
      961 ccgcccgcgt acgggccgca gtggtttgcg aaggggcctg cggagatccc gccgcgggct
     1021 gcatccgccc cgcccggcgg caaaagcctc aggccgccgc cgcggccttg cggcgcagca
     1081 ccgcgcgccg gtagatcagc ccggcgatca ccgtgagcac gccgcacacc accagcgtgg
     1141 ggcgcacgcc aaagacgtgg gcggcgctgc ccaccgtgaa gctgcccagg ggcgccacgc
     1201 ccaggaaggc catggagaac agggccatca cccggccgcg gaaggcgtta tccacctgcg
     1261 tctggatcag cgtattgctg ccggcaatgg tcaggatgat gctgccgccc agggccagca
     1321 gcaccggcac cgccatcccc acgccgggcg acagcgcgaa gaggatcacc gccacgccca
     1381 ccgtgggcgc cgccacggcc accttctcgg ccagcccgcc ggtgtccgcg cgccccgcca
     1441 gataaaagct ggccaccagc gaacccgccc ccgcgctgcc caccagcagg ccgaagaggc
     1501 gcgcgccgcc gccgaactcc tcccgggcga agaggggcat cagcaccacg tagggcgccg
     1561 cgcacaggct gatcgccgcc accagccgca gggacgcgcg gatctcccga tgggccatcg
     1621 cgtagcgcca gccctcccgc agggcctgca agggcggcgg cgcgggcggg cgatcccccg
     1681 cggcaggcag gcggatcgcc cgcagcgcca tcaacaccgc cagataggac gccgcgttga
     1741 gcagaaagca caccgcctcg cccacctccg ccaccacaaa gcccgccagc gccggcccca
     1801 cgaagcgcgc cgtgttcatc agaaaggaat tgagggcgat ggcattgggc agatcgtccg
     1861 gatcgtccac cagctgcacc gcgatcgcct gccgcgccgg cacatccagc gcgttgatgc
     1921 agcccagcac aaaggccagc ccgaccagat gccccggcgt cgccgcatcc ttccacgcca
     1981 gcgccgccag cagcagcgcc tgcaccatgg acaaggcctg cgtcgccagc aacacccgcc
     2041 gccggtccag ccggtccgtc accaccccgc cgaaggcccc gaacaacaga atcggcgcct
     2101 gactcgcaaa ccccaccatc cccaacacaa acgccgaccc cgacaagcga tacgccagcc
     2161 acaccatcgc gatctgctgc atccacgtgc ccgcgaggga ga
//
LOCUS       DAOQPA010000250         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_43998_length_2188_cov_13.955931, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000250 DAOQPA010000000
VERSION     DAOQPA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43998_length_2188_cov_13.955931"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKX01_17495"
     CDS             complement(<1..349)
                     /locus_tag="PKX01_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77984.1"
                     /translation="MQTASLFMMGASNNENEGMPFDCTCNALSLPTAKALYARFGEYP
                     GMSSSYRRCDRTATCKKNTVGVREGPSSDGSDAIASRDAASRHIREGWTKKKRRFGAA
                     EESRTLDLNLGKVA"
     gene            327..1001
                     /locus_tag="PKX01_17500"
     CDS             327..1001
                     /locus_tag="PKX01_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ77985.1"
                     /translation="MNKLAVCILSSVAAVGVLSGCATEESRSLAVAKPAAAARPYSGN
                     RTLISVGKFDNRSSFMRGIFSDGVDRLGSQAKTILITQLQQSNRFNVLERENLSELKQ
                     EAALSQTGQQLKGARFVVTGDVTEFGRKEVGDHQLFGILGRGKQQIAYAKVSLNVVNV
                     QTSEVVYSAQGAGEYSLSNREVIGFGGTASYDSTLNGKVLDLAIRDAVDALVGGIESG
                     AWRVAE"
     gene            998..1384
                     /locus_tag="PKX01_17505"
     CDS             998..1384
                     /locus_tag="PKX01_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HMZ77986.1"
                     /translation="MSAKQLPMTARALSRSVGAAGLTLALVLLAGCANRPQSLYYWGT
                     YQGQVYGQLQGTISPQAQIQSLEETREKARAEGKALPPGFQAHLGMLYGESGQLDRFQ
                     QNLDAEKRQFPESATFVDFMMDKLKR"
     gene            1406..2092
                     /locus_tag="PKX01_17510"
     CDS             1406..2092
                     /locus_tag="PKX01_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HMZ77987.1"
                     /translation="MKIAHYLKTVSVALGAVLLSACAHQSGSKPYDYAAFRESRPASI
                     LVLPPLNESPEVKASAGMLSRVTFPLAESGYYVFPVALVDEAFRQNGLTSPADIHAVS
                     LEKLRSIFGADAVLYIKVTEYGTTYKVIASETRVAAEGRLVDARTGAALWSGQARASS
                     AETQNNSSGGLIGALVTALMTQIADTLNDRGVAIAGLTSLRLLSAGTPNGILYGPRSP
                     KYQKDGSPAP"
BASE COUNT          383 a          700 c          703 g          402 t
ORIGIN      
        1 gcgcaacctt gccaaggttg aggtcgagag ttcgagactc ttctgccgct ccaaatcttc
       61 ttttcttctt cgtccagccc tcccggatgt gtcgggatgc ggcatcgcga ctggcgattg
      121 cgtccgatcc gtccgagctc ggtccttctc gcacgcccac agtgtttttc ttgcaggtcg
      181 cggtacggtc acacctcctg tacgacgaag acatccccgg atattcacca aatcgggcat
      241 acaaggcctt ggcggtcggc agggacaggg cattgcaggt acaatcgaag ggcatacctt
      301 cattctcatt gttacttgcg cccatcatga acaagcttgc tgtctgcatt ctgtcttccg
      361 tcgccgcggt tggtgttctg tcgggctgcg ccacggaaga gtcccgctcc ctcgccgttg
      421 ccaagcccgc tgcagcggcc cggccctata gcggcaaccg gacgctgatc tccgtcggta
      481 agttcgacaa tcgttcgagc ttcatgcgcg gcatcttctc ggacggcgtg gaccgcttgg
      541 gcagtcaggc caagacgatc ctgatcaccc agcttcagca gagcaaccgc ttcaatgtgc
      601 tggagcgcga gaacctctct gaactgaagc aggaggccgc gctgagccaa accggccagc
      661 agctcaaggg tgcgcgcttc gtggtgaccg gcgacgtgac cgaattcggc cgcaaggagg
      721 tgggtgacca tcagttgttc ggcatcctgg gacggggcaa gcagcagatc gcctatgcca
      781 aggtgtcgct caacgtggtg aatgtgcaga cctccgaagt ggtctattcg gcccaaggcg
      841 cgggcgaata cagcctttcc aaccgggaag tgatcggctt tggcggaacg gccagctacg
      901 attccacgct caacggcaag gtgctcgatc ttgccatccg cgacgcggtg gacgccctgg
      961 tcggcggcat cgaatccggc gcctggcgtg tggccgaatg agcgcgaagc aactgccgat
     1021 gacggcgcgc gcgctgtcgc gttccgtggg ggctgccggc ctgacgctgg cccttgtgct
     1081 gctggccggt tgcgccaacc ggccccagag cctgtactac tggggcacct atcagggtca
     1141 ggtctacggc cagttgcagg gcacgatcag tccgcaggca cagatccagt ccctcgagga
     1201 gacccgcgaa aaggcgcgcg ccgagggcaa ggccctgccg ccgggcttcc aggcccatct
     1261 ggggatgctc tacggcgaga gcgggcagct tgatcgcttc cagcagaatc tcgatgccga
     1321 gaaacgccag tttcccgaga gcgcgacctt cgtcgatttc atgatggaca agctcaaacg
     1381 ctgaccgccc ccacgcccaa ttgccatgaa gatcgctcat tatctgaaga cggtgtccgt
     1441 cgcgctgggg gcggtgctgc tctccgcctg cgcccaccaa tccggcagca agccctacga
     1501 ttacgcggct ttccgcgaga gccggccggc gtccatcctg gtgctaccgc cgctcaatga
     1561 atcgccggag gtgaaggcga gcgccggcat gctgtcgcgg gtcacgtttc cgctggctga
     1621 atcaggctat tacgtgttcc cggtcgcgct ggtggacgag gccttcaggc agaacgggct
     1681 gacctcgccc gctgacatcc acgcggtgtc cctggagaag ctgcgttcga ttttcggagc
     1741 ggacgcggtg ctgtacatca aggtgaccga atacggaacg acctacaagg tcatcgccag
     1801 cgagacgcgc gttgcggcag aaggtcgcct ggtggatgcg aggacaggcg ccgctctgtg
     1861 gtccggtcag gcgcgtgcat cgagcgcgga gacgcagaac aactcgagcg gcggcttgat
     1921 cggcgcgctg gtgacggcac tgatgaccca gatcgccgac acgctcaatg accggggcgt
     1981 tgcgatcgcc gggctgacga gcctgcgcct gctgtcggct ggcacgccga acggcatcct
     2041 ctacggcccc cggtcgccga agtaccagaa ggacggttcg ccggccccct gacagcgccg
     2101 tcgcgccgcc accgcccggc gccggggcag tcagcgttca gccgatggtg cccggcttgt
     2161 tgccgagcat ggcacgcagg ttggcgag
//
LOCUS       DAOQPA010000251         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_44125_length_2185_cov_13.813146, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000251 DAOQPA010000000
VERSION     DAOQPA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44125_length_2185_cov_13.813146"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKX01_17515"
     CDS             <1..366
                     /locus_tag="PKX01_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77988.1"
                     /translation="WCWRSIAMGSASTGSGSVRKYARHSAARWRALISEFEASGVSQQ
                     AFCTRHGLAKSTFELWRRKLRGARNTDVAGVRPEALFVELTAPVVDKTEVLPATPAWE
                     VELDLGAGVVLRLRRGAPC"
     gene            360..719
                     /gene="tnpB"
                     /locus_tag="PKX01_17520"
     CDS             360..719
                     /gene="tnpB"
                     /locus_tag="PKX01_17520"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HMZ77989.1"
                     /translation="MLSISSASRIWLCLEPTDMRCSFDGLSARVKQHLGEDPLGSSSQ
                     WFAFLNRRATQIKVLGFETGGYWIWSKRLERGHFARLMTRDGRVKRALCATEFLALLE
                     GVDVEIKRQRKRYRTAA"
     gene            774..>2185
                     /locus_tag="PKX01_17525"
     CDS             774..>2185
                     /locus_tag="PKX01_17525"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HMZ77990.1"
                     /translation="MNTASRIAELEHTNAQLTVQMAAMTQKFGAEIATLKQQLDWFKR
                     QLFGQKSEKQLNIDPAVQGNLLDALGVLEPPQNSPPPPEQSITYTRRGKVRDAAVNDS
                     GLRFGPDVTRDVITIVDPAIAAVPEDRRELVSERVSYRLAQRPGSYGIIEYHYQTWKL
                     VDEQRIVSTPAPASVLERCVADVSLLAGMLVDKFCFHLPLYRQHQRIAQCGIQLSRTS
                     LSNWSGRAIDLLRPIAAAQHAHILSGSVVAMDETPIKAGREKQGKMRQAYLWPIYGED
                     NEIVFHYAPTRAHHHVEKILGPDFQGTLLSDGYAAYSAYAGRKGGVTHASCWAHCRRG
                     FEQARDAEPQAAAEALAMIAALYRHEQIIREQNLDREHRLAYRTQHSEPIVDRFWRWC
                     DDQCHRMNLLPSNPLAKAIQYAKARVASLRVFLSDPDVPIDTNHLERSLRAVPMGRRN
                     WLFCWTEVGAERVAVIQSLLV"
BASE COUNT          410 a          689 c          724 g          362 t
ORIGIN      
        1 tggtgttgga ggagtatcgc catgggttcg gcatcgacgg gttcaggttc tgtgcgcaag
       61 tatgcgcgtc acagcgcggc acggtggcgc gcgttgatca gcgagttcga ggcgagcggc
      121 gtttcgcagc aggcgttctg cacccgccac ggcctggcga agagcacctt cgagctgtgg
      181 cggcgcaagc tacgcggggc ccggaacacc gatgtggcgg gcgttcggcc cgaggcgctg
      241 tttgtcgaac tgaccgcgcc ggtggtcgac aagaccgagg tgctaccggc cacacctgcg
      301 tgggaggtag agctcgacct gggtgcgggt gtcgtgctgc ggctgcgccg gggcgcccca
      361 tgctgagcat cagcagtgca tcgcgcatct ggctgtgcct ggaacccacc gacatgcgct
      421 gctcgttcga tgggctgtcg gcacgggtga agcaacacct gggcgaagac ccactgggca
      481 gcagtagcca atggtttgcg tttctcaacc gccgcgccac gcagatcaag gtgctcggct
      541 tcgagacggg cggctactgg atctggtcga agcgactcga gcgcggacac tttgcccgcc
      601 tgatgacacg cgatggacgc gtgaaacgcg cactgtgcgc caccgagttt ctggcgctgc
      661 tcgaaggcgt cgacgtcgag ataaaaaggc agcgaaaacg ctaccgaaca gcggcatgaa
      721 tgagggcatt ccggtacaat ggcggcacgg ttttcctgca gtattttccg cccttgaaca
      781 ccgcttcgcg catcgccgaa ctcgaacata ccaatgcgca actcacggtg cagatggccg
      841 cgatgacgca gaagtttggc gccgaaattg cgacactgaa gcagcagctc gactggttca
      901 agcgccagct cttcgggcag aagagcgaga agcagctgaa catcgatcct gccgtgcagg
      961 gcaacctgct cgatgcgctc ggggtgcttg aacctccaca gaattcccca ccgcctcccg
     1021 agcagtcgat cacctacacg cgccggggca aggtgcgcga tgcggcagtg aacgactcag
     1081 gattgcgctt tggccccgat gtcacccgcg acgtgatcac catcgtcgat ccggcgattg
     1141 ccgccgtacc cgaggatcgg cgcgagctgg tctccgagcg cgtcagctac cgcctggcgc
     1201 aacgcccggg cagctacggg atcatcgagt accactacca gacctggaag ctggtcgacg
     1261 agcagcgcat cgtcagcacc ccggcaccgg cgagcgtgct cgagcgctgc gtggccgatg
     1321 tgagcctgct ggccggaatg ctcgtcgaca agttttgctt ccacctgccg ctgtatcgcc
     1381 agcaccagcg gatcgcccag tgtggcatcc agctgagccg caccagtctc agcaactgga
     1441 gcggacgggc catcgatctg ctgcgcccca tcgcagcggc ccagcacgcc cacatcctca
     1501 gcggatcggt ggtagcgatg gacgagactc cgatcaaggc gggacgcgag aagcagggca
     1561 agatgcgcca ggcctacctc tggccgatct acggcgagga caacgagatc gtcttccact
     1621 acgcgccgac ccgggcgcac caccatgttg agaagatcct ggggccggac ttccagggaa
     1681 cgctgctctc ggacggctac gccgcctaca gcgcctatgc ggggcgcaag ggtggcgtca
     1741 cccacgcgag ttgctgggcg cattgtcgcc gaggcttcga gcaggcccgg gatgccgagc
     1801 cgcaggcggc ggccgaggcg ctggccatga ttgccgcgct gtaccggcac gagcagatca
     1861 tccgggaaca gaacctggac cgggaacaca ggctcgctta ccgaacacag cacagcgagc
     1921 cgatcgtcga ccgcttctgg cgctggtgtg acgaccagtg ccatcgcatg aatttgctac
     1981 cgagtaaccc gcttgccaaa gcgatccagt acgcgaaggc gcgggtggct agcctgcggg
     2041 tgttcttgtc ggatccggat gtgccgatcg acaccaacca cctggagcgc agcctgcggg
     2101 cggttccaat gggacggcgc aactggctct tctgttggac cgaggtcggt gccgagcggg
     2161 tagcggttat ccaaagcctg ctggt
//
LOCUS       DAOQPA010000252         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_44857_length_2166_cov_11.014685, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000252 DAOQPA010000000
VERSION     DAOQPA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44857_length_2166_cov_11.014685"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2166)
                     /locus_tag="PKX01_17530"
     CDS             complement(<1..>2166)
                     /locus_tag="PKX01_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HMZ77991.1"
                     /translation="SARSNPVSYVGAWDAIRKLFAALPESKERGYTAGTFSFNSGTGR
                     CPTCTGSGFEHVEMQFLSDVYLRCPDCDGQRYRPEILALAWHGHSVADVLEMTVREAL
                     SAFAGQKAVLAALAPLADVGLDYLRLGQPVPTLSGGEAQRLKLAGFLAEAAQKKSARR
                     GADTGPGLLFLFDEPTTGLHFQDVATLLAAFEKLLDAGHSLVVIEHNLDVIAAADWLL
                     DLGPEGGRGGGRLVAEGTPADLMACPASHTGRALTDYAAQLAALQAAPRVAEAPPRYR
                     PVAAPAIEIRHARHHNLRNISLSIPRDRFTVVTGLSGSGKSTLAFDIVFGEGQRRYLE
                     SLNAYARQFAQPSARPDVDAIFGIPPTVAIEQRVSRGGRKSTVGTLTEIQPFLRLLYV
                     KLGTQYCPDCDVPVTPQSFEAIVAQLQRDFAGRSVELLAPLVVNRKGLYTDLAKWARG
                     KGFEQLRVDGDYLPTRKWPRLDRYKEHNIELPVGMVRIGPDTEVALRDAVQQALEHGK
                     GVLKALPLGDAGARPLTLSTLRACPDCGTSFPEPDPRLFSYNAKHGWCPDCYGTGLRL
                     AGKVDNPDDLDLGEADAVSDETCPSCHGARLNPVARAVRFRDLGLHELASLAVGQVAH
                     FFGGLALTPREADIARDLVAEIRGRLDFLQQVGLAYLALDRAAPTLSGGEAQRIRLAA
                     QLGSNLTGVCYILDEPTIGLHPRDNRLLLDTLEALRARG"
BASE COUNT          305 a          667 c          886 g          308 t
ORIGIN      
        1 tgccgcgggc gcgcagggcc tccagggtgt cgaggagcag gcggttgtcg cggggatgca
       61 ggccgatggt gggctcgtcc aggatgtagc acacgccggt gaggttggag ccgagctggg
      121 cggccagccg gatgcgctgg gcttcgccgc cggagagggt cggcgcggcc cggtcgagcg
      181 ccaggtaggc gaggccgacc tgctgcagga agtcgagccg gccgcggatt tcggccacca
      241 ggtcgcgggc gatgtcggcc tcgcgggggg tgagggcgag gccgccaaag aagtgcgcca
      301 cctgccccac cgccaggctg gcgagttcgt gcaggcccag gtcgcggaag cgcacggcgc
      361 gggccaccgg attgaggcgg gcaccgtggc agctcggaca ggtctcatcg ctgacggcgt
      421 cggcttcgcc cagatcgaga tcgtccgggt tgtccacctt gccggccagg cgcaggccgg
      481 tgccgtagca gtccgggcac cagccgtgct tggcgttgta ggagaacaga cgcgggtcgg
      541 gctcggggaa gctggtgccg cagtccgggc aggcgcgcag ggtggagagg gtcagcggcc
      601 gggcgccggc gtcaccgaga ggcagggcct tgagcacgcc cttgccgtgt tcgagggcct
      661 gttgcacggc gtcgcgcagg gcgacctcgg tgtccggacc gatccgcacc atgcccaccg
      721 gcagttcgat gttgtgctcc ttgtagcggt cgaggcgtgg ccacttgcgg gtgggcaggt
      781 agtcgccatc gacccgcagc tgctcgaagc ccttgccccg cgcccacttg gccaggtcgg
      841 tgtacaggcc cttgcggttc accaccaggg gggcgaggag ttccacggag cggccggcga
      901 aatcccgctg gagctgggcg acgatggcct cgaagctctg gggcgtgacc ggcacgtcgc
      961 agtccgggca gtactgggtg cccagcttga cgtaaagcag gcgcaggaag ggctggatct
     1021 cggtgagggt gccaaccgtg ctcttgcgcc cgccgcggct gacccgctgc tcgatggcaa
     1081 cggtgggcgg aatgccgaag atggcgtcca cgtccggccg cgccgagggc tgggcgaact
     1141 ggcgggcgta ggcgttgagg gattccaggt agcggcgctg gccttcgccg aagacgatgt
     1201 cgaaggccag cgtggatttg cccgatccgg acaggccggt gaccacggtg aagcggtcgc
     1261 gcgggatgga caggctgatg ttgcgcagat tgtggtggcg ggcgtggcgg atctcgatgg
     1321 ccggtgccgc caccggccga tagcgcggtg gcgcttcggc cacccgcggg gcggcctgca
     1381 gggcggcgag ctgggccgca tagtcggtga gggcgcggcc ggtgtgggag gccgggcagg
     1441 ccatgagatc tgccggggtg ccctcggcga ccaggcgacc accgccgcgg ccgccttctg
     1501 ggcccaggtc gaggagccag tcggcggcgg cgatcacgtc caggttgtgc tcgatgacga
     1561 cgagggagtg gcccgcgtcg aggagcttct cgaaggccgc caggagcgtg gccacgtcct
     1621 ggaagtgcag gccggtggtg ggctcgtcga agaggaagag caggcccggc ccggtatcgg
     1681 cgccccggcg tgcggatttc ttctgggccg cctcggcgag gaaaccggcg agcttgaggc
     1741 gctgggcctc gccgccggag agggtgggca ccggctggcc caggcgcagg tagtcgaggc
     1801 ccacgtcggc caggggcgcc agggcggcga gcaccgcctt ctgcccggcg aaggcgctga
     1861 gggcttcgcg gacggtcatc tccagcacgt cggcgacgga gtggccgtgc caggcgaggg
     1921 cgaggatctc cggccggtag cgctggccgt cgcagtccgg gcagcgcagg tagacgtcgg
     1981 acaggaactg catctccaca tgctcgaagc ccgagccggt gcaggtgggg cagcgtccgg
     2041 tgccggagtt gaagctgaag gtgccggcgg tgtaaccccg ctctttggac tccggcaggg
     2101 cagcgaagag tttgcggatg gcgtcccagg cgcccacgta gctcaccggg ttggagcggg
     2161 cgctct
//
LOCUS       DAOQPA010000253         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_46769_length_2119_cov_17.579942, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000253 DAOQPA010000000
VERSION     DAOQPA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46769_length_2119_cov_17.579942"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PKX01_17535"
     CDS             <1..1116
                     /locus_tag="PKX01_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ77992.1"
                     /translation="TWEECETEYNNARGVECYERLKKKGSTGNSVFQPVSTGITYSVL
                     EKVAQDKIPMVTIGYGRTDAADGRVFPWVFPLITTYWSQASAIVNYIGSLEGGMDRLK
                     GKKIGLLYHDSAYGKESHAILDKLAAKHGFEVTKIAVAHPGNEQQSQWLQIRQLKPDY
                     VILWGWGVMNPTAIKAAAKTGFPREKLIGVWWSGAEEDTIPAGPAAKGFIAAGFNVAG
                     ANYPVIADIKKHVYGKSKGNMEDKTRIGSIYYNRGVVHGLITVEAIRKAQDKFGKGKA
                     LTGEQVRWGFENLNLDDARLKALGATGFLPPLKISCADHEGPGKVKFQQWDGSQWKVI
                     SDWVESDRPLVRGMIEESAMAYAKEKGIKPRDCSKEN"
     gene            1314..2114
                     /locus_tag="PKX01_17540"
     CDS             1314..2114
                     /locus_tag="PKX01_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ77993.1"
                     /translation="MSYLSINNIEVIYDHVILVLKGVSLEVPQGKIVALLGANGAGKS
                     TTLKSISNLLRAERGDVTKGSIEFKGDRVDCMTPADLVKRGVIQVMEGRHCFGHLSIE
                     ENLLTGAYTRSLSRSALKENLERVYAYFPRLKTRRTSQAGYTSGGEQQMCAIGRALMA
                     RPEMILLDEPSMGLAPQIVEEIFEIVKDLNSKENVSFLLAEQNTMVALRYADFGYILE
                     NGRVVMEGEAEALRTNEDVKEFYLGLSSEGRKSFRDVKHYRRRKRWLS"
BASE COUNT          414 a          691 c          706 g          308 t
ORIGIN      
        1 acctgggaag agtgcgagac cgagtacaac aacgcccgcg gcgtggagtg ctacgagcgc
       61 ctgaagaaga agggcagcac cggcaattcg gtgttccagc cggtgtccac gggcatcacc
      121 tattcggtgc tggaaaaggt ggcccaggac aagatcccca tggtcaccat cggctacggc
      181 cgcaccgacg ccgccgacgg ccgcgtcttc ccgtgggtgt tcccgctgat caccacctac
      241 tggtcccagg cctcggccat cgtcaattac atcggctcgc tggagggcgg gatggaccgg
      301 ctgaagggca agaagatcgg cctgctctac catgactccg cctacggcaa ggagtcccac
      361 gccatcctcg acaagctggc cgccaagcac ggcttcgagg tgaccaagat cgccgtggcc
      421 cacccgggca acgagcagca gtcccagtgg ctgcagatcc gccagctcaa gccggactac
      481 gtgatcctgt ggggctgggg cgtgatgaac ccgaccgcca tcaaggccgc cgccaagacc
      541 ggcttccccc gcgagaagct gatcggtgtg tggtggtccg gtgccgaaga ggacaccatc
      601 cccgccggcc cggccgccaa gggcttcatc gccgcgggct tcaatgtggc cggcgccaac
      661 tacccggtga tcgccgacat caagaagcat gtgtacggca agagcaaggg caacatggaa
      721 gacaagaccc gcatcggctc catctactac aaccggggcg tggtgcacgg gctcatcacc
      781 gtcgaggcga tccgcaaggc ccaggacaag ttcggcaagg gcaaggccct caccggcgag
      841 caggtgcgct ggggcttcga gaacctcaat ctggacgacg cccgcctgaa ggccctgggc
      901 gccaccggct tcctgccgcc cctcaagatc agctgcgccg atcacgaagg gccgggcaag
      961 gtgaagttcc agcagtggga cgggagccag tggaaggtga tctccgactg ggtggaatcc
     1021 gaccggcctc tggtgcgcgg catgatcgaa gaatcggcca tggcctacgc caaggaaaag
     1081 ggcatcaagc cccgcgactg ctccaaggaa aattgacggg ggcggcgcgc acgcggctgc
     1141 gcgggccgat cccgcggcgc tgaggcccgc cgtacgcccg tacggctgcg cgtcccgcca
     1201 cggcctcgag ccgcgcgtga cgcgtgcgcg caccgaacct tgggtgactg ctttcttatc
     1261 gcaaccggaa cccggacggc cccagccgtc cgcacagggg gatggccgac gccatgtctt
     1321 acctcagcat caacaacatc gaggtcatct acgaccacgt gatcctcgtg ctcaagggcg
     1381 tctccctgga ggtgccccag gggaagatcg tggccttgct gggcgccaac ggggccggca
     1441 agagcacgac gctcaagtcc atctccaacc tgctgcgggc cgagcggggc gatgtgacca
     1501 agggcagcat cgagttcaag ggcgaccggg tggactgcat gacgccggcc gatctggtga
     1561 agcgcggggt gatccaggtg atggagggcc gccactgctt cggtcacctg tccatcgagg
     1621 agaacctgct caccggcgcc tacacccgca gcctgtcccg cagcgcgctg aaggagaacc
     1681 tggagcgggt gtacgcctac tttccgcgcc tcaagacgcg acgcacgtcc caggccggct
     1741 atacctcggg gggcgagcag cagatgtgcg ccatcggccg cgccctgatg gcccggcccg
     1801 agatgattct cctcgacgaa ccctccatgg gcctcgcgcc gcagatcgtg gaggagatct
     1861 tcgagatcgt gaaggacctg aacagcaagg agaacgtgag cttcctcctc gccgagcaga
     1921 acaccatggt ggcgctgcgc tacgccgact tcggctacat cctggagaac ggccgcgtgg
     1981 tgatggaagg cgaagccgaa gccctgcgca ccaacgagga cgtgaaggag ttttatctcg
     2041 ggctgtcgtc cgaggggcgc aagagcttcc gggacgtgaa gcattaccgg cggcggaagc
     2101 ggtggctgtc gtgaggggg
//
LOCUS       DAOQPA010000254         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_47022_length_2113_cov_18.935860, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000254 DAOQPA010000000
VERSION     DAOQPA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47022_length_2113_cov_18.935860"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..618)
                     /locus_tag="PKX01_17545"
     CDS             complement(7..618)
                     /locus_tag="PKX01_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HMZ77994.1"
                     /translation="MSASKLEVWFEFASSYSYLSVMRIAPLAQAAGVELVWRPFLLGP
                     VFLSLGWNDSPFNIYPPKGRYMWRDLARLAARYGLPLRMPSRFPRNGVLAARVALAGA
                     DQPWVGDFARAAMAANFAEDRDIADEAVIRALLAGLGLDPDTWIAAATTPENKLALRR
                     QTERAAELGLFGAPSFITPDGELFWGNDRLEDALSWVKDGPTL"
     gene            complement(684..1034)
                     /locus_tag="PKX01_17550"
     CDS             complement(684..1034)
                     /locus_tag="PKX01_17550"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HMZ77995.1"
                     /translation="MSIHPPPQLLSLQRYEARILEDAVGTLVACLSGCVWLTQHGDSR
                     DIILHAGDSFRIERPGCVVMAAGRPTRLHLVAPPAEAPRPARARAWWARLFSPRWHAA
                     ARHLMQGGGAVPDR"
     gene            1197..>2113
                     /locus_tag="PKX01_17555"
     CDS             1197..>2113
                     /locus_tag="PKX01_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ77996.1"
                     /translation="MNTPLLHLPPLDPLRGFVAAARALSFTRAAEALCLTQSAVSRQV
                     QTLEEGLGVSLFVRGTRTLALTPEGERLARLAEDWLREYADYAASLRDTRLRPPVTVT
                     ATIGISALWLLPRLRDFQALHPDVDVRLASSNRVVELQREGIDLALRYCPDRDVPDGA
                     VRLFGETVFPVASPALGVSALDAATLPELTLLYFDMPGFGWLNWDDWLTADGLGQLRP
                     RGTLHFNHYDQLIQAAVAGQGVAIGRAELVSPLLERGQLRRIGCAHREVSGRGYWLLQ
                     ADAMPRDDVRRFAAWIVEAARQTRDGLATT"
BASE COUNT          315 a          757 c          742 g          299 t
ORIGIN      
        1 gcccgcctac agcgtcggcc cgtccttcac ccagctcagc gcatcctcca gccggtcgtt
       61 gccccagaac agctccccgt ccggcgtgat gaaggacggg gcgccgaaga ggcccagttc
      121 ggcggcccgt tcggtctggc ggcgcagggc gagcttgttc tcgggcgtgg tggcggcggc
      181 gatccaggtg tcgggatcga gccccaggcc ggcgaggagt gcgcggatca ccgcttcgtc
      241 ggcgatgtcc cggtcctcgg cgaagttggc agccatcgcg gcgcgcgcaa agtcaccgac
      301 ccacggctga tccgcgccgg ccagggcgac ccgcgcggcg agcacgccat tgcgcgggaa
      361 gcgcgagggc atgcgcaggg gcaggccgta gcgggcggcc aggcgtgcca gatcgcgcca
      421 catatagcga cccttgggcg ggtagatgtt aaagggcgaa tcgttccagc cgagggacag
      481 aaagaccggc ccgaggagga agggccgcca caccagctcg acccctgcag cctgcgctag
      541 cggcgcgatg cgcattacgc tgaggtagga ataggagctg gcgaactcga accagacttc
      601 cagtttcgat gcggacatgg cggtctccca gaatgacgat tcggtcagtg tagcccgcgc
      661 cagcgacgcg gtcatgacgc cgctcaacgg tccggaacgg caccgccgcc ctgcatcaga
      721 tgacgcgccg cggcgtgcca gcggggcgag aacaggcgcg cccaccaggc gcgggcccgg
      781 gccggccgcg gcgcctctgc cggcggcgcc accaggtgca ggcgcgtcgg ccggccggcg
      841 gccatcacca cgcagccggg gcgttcgatg cgaaagctgt caccggcgtg caggatgatg
      901 tcgcggctgt ccccgtgctg ggtgagccac acgcagcccg acaggcaggc caccagggtc
      961 ccgacggcat cctccaggat gcgcgcctcg tagcgctgca gactgagcag ttggggtggg
     1021 ggatgaatgc tcatgatgct ctcctggggc tgcgtgaatg cgtcttgcgc attacactgg
     1081 ggactccggc cggtcggccc gccgggctgc gccatccaca cgtggcgctt ccagtattgg
     1141 ccgccgagca gcaccggaca aacgcattcg gagcatcctg ttcatgagaa atccgcatga
     1201 atacgcccct cctccacctg ccgccccttg atcccctgcg gggcttcgtg gcggccgccc
     1261 gcgccctcag cttcacccgc gccgccgagg ccctgtgcct gacccagtcc gcggtgagcc
     1321 gccaggtgca gactctggag gagggcctcg gggtgagcct cttcgtgcgc ggcacgcgga
     1381 ccctcgccct gacgccggaa ggcgagcgtc tggcccggct ggcagaggac tggcttcggg
     1441 aatacgccga ctacgcagcc agcctgcggg acacccggct gcgtccgccg gtgacggtga
     1501 ccgccaccat cggcatctcg gccctgtggc tgctgccgcg cctgcgggat ttccaggccc
     1561 tgcacccgga cgtagacgtg cgcctcgcca gcagcaaccg cgtcgtcgaa ctgcagcggg
     1621 aaggcatcga tctggccctg cgctactgcc cggaccggga cgtgccggac ggcgccgtgc
     1681 ggctcttcgg tgaaaccgtc tttccggtgg cgagccccgc tctgggcgtg agcgccctgg
     1741 acgccgccac cctgcccgag ctgaccctgc tctatttcga catgcccggc ttcggctggt
     1801 tgaactggga cgactggctg acggccgacg gcctgggaca gctgcggccg cggggcaccc
     1861 tgcacttcaa ccactacgac cagctcatcc aggccgcggt cgccggccag ggcgtcgcca
     1921 tcggccgcgc cgagctcgtc tcacccctgc tggaacgcgg ccagctgcgg cgcatcggct
     1981 gcgcccaccg ggaagtcagc gggcgcggct actggctgct gcaggccgac gccatgcctc
     2041 gcgacgacgt gcgccgcttt gccgcgtgga tcgtggaggc ggcacgccag acccgcgacg
     2101 gtctggccac gac
//
LOCUS       DAOQPA010000255         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_47023_length_2113_cov_13.680758, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000255 DAOQPA010000000
VERSION     DAOQPA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47023_length_2113_cov_13.680758"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..637
                     /locus_tag="PKX01_17560"
     CDS             92..637
                     /locus_tag="PKX01_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77997.1"
                     /translation="MSPHRAPPPLGLDFVGGLRRPGWLGWAVLAFGVIAAVGVLHEYD
                     VLETRVAEAAHALTRTKHGLDLRASGVDRTPVTEQEARPARAVAQLLQRDWRAVLGVL
                     ETASDDPGIALLALDQEGARGGLRLTGEGRSLPEVFAYVKRLEAGGVLREVRLGSYEF
                     HASGPLQVVGFTVTARWEVAP"
     gene            634..1203
                     /locus_tag="PKX01_17565"
     CDS             634..1203
                     /locus_tag="PKX01_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77998.1"
                     /translation="MSLRLTGARWATLHESAVRTFRAAGWAGVVGVALTVFAVCFDLS
                     GNRALEAQADALEQETRSLRLRLRTHPEGVAVPERTRLERFYGAFPATDALPDLLVRL
                     HGYALARGLKLDRADYRSAPEGGTPLERVTLELPARGPYPVLRTWLADVLAEMPEVAL
                     DGLSLRRASMATPEVETQVRFVVFLRRQP"
     gene            1200..1694
                     /locus_tag="PKX01_17570"
     CDS             1200..1694
                     /locus_tag="PKX01_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ77999.1"
                     /translation="MKRVHGLLAGLALTLLAAWWAAGLEDEPATEAPRRAVQRKAASV
                     RSAPRVDLAALAASRPALADGAAGAPVPDLFPVRSFAPPPPPPPPPPTPTAPPLPFRY
                     TGMLEEGGRHAAFLEQGEQIRVVRVGDTLDGRYRVTAVSRARIDLIYLPLNEPQSLVT
                     GAMP"
     gene            1691..>2113
                     /locus_tag="PKX01_17575"
     CDS             1691..>2113
                     /locus_tag="PKX01_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspD"
                     /protein_id="HMZ78000.1"
                     /translation="MTSRRLPRLLRRLAPLALTVLLAACAGNKALEESRRSFATQSPD
                     VALIDLRSRVEADPRNLELRAYYLRQREALTTRQLAMADQARGAGRFEEAENALALAR
                     QFDPNNPRVVAGVDAIAAQRQRNQLAAEADRRLAAGDFA"
BASE COUNT          247 a          778 c          788 g          300 t
ORIGIN      
        1 cccagggcac cctctatctt gcgggtgcgg ccgcgaccgc gacggcgctg cccgctggct
       61 ggaccctggc cggcgtggaa ggcggtgcgt catgagcccg catcgcgcgc cgccgccgct
      121 ggggctggat ttcgtcggcg gcctgcgccg gcccggctgg ctgggctggg cggtgctggc
      181 cttcggcgtg atcgcggcgg tcggcgtgct ccacgaatac gatgtgctgg agacccgcgt
      241 ggccgaagcg gcccatgctc tgacgcggac caagcacggg ctcgacctgc gcgccagcgg
      301 cgtcgatcgc acgccggtga ccgagcagga ggcgcgcccc gcgcgggccg tggcacagct
      361 gctgcagcgg gactggcgcg ccgtgctggg ggtgctggaa acagccagcg acgatcccgg
      421 catcgccctg ctggcgctcg accaggaggg cgcgcgcggt ggcctgcgcc tgaccggcga
      481 ggggcgcagc ctgcccgagg tcttcgccta cgtgaagcgt ctcgaggcgg gtggcgtgtt
      541 gcgcgaggtg cgtctgggca gctatgagtt ccatgccagc ggaccgcttc aggtcgtcgg
      601 cttcaccgtg accgcccgct gggaggtcgc gccatgagcc tccgtctgac cggcgcccgg
      661 tgggcgaccc tgcacgaatc cgcggtgcgg accttccgcg cggcgggctg ggccggcgtg
      721 gtgggcgtgg ctctgaccgt gtttgccgtg tgtttcgacc tgagcgggaa ccgggctctg
      781 gaagcccagg ctgacgccct ggagcaggaa acccgcagcc tgcgcctgcg cctgcgcacc
      841 catccggagg gcgtcgccgt gcctgagcgg acgcggctgg agcgcttcta cggggccttc
      901 cccgcgaccg atgcgctccc tgacctgctg gtgcgcctgc atggctacgc gctggcgcgg
      961 ggcctcaagc tggaccgggc cgattaccgt tctgcgccgg agggcggcac gccgctggag
     1021 cgggtcactc tggagcttcc cgcgcgcggc ccctacccgg tgctgcgcac ctggctggcc
     1081 gacgtgctgg cggagatgcc cgaagtggcg ctggacggcc tgtccctgcg gcgtgcgtcc
     1141 atggcgacgc cggaggtgga gacgcaggtg cgttttgtgg tgttcctgag gcgccagcca
     1201 tgaagcgcgt gcatgggctg ctcgccggac tggcgttgac cctgctggcc gcctggtggg
     1261 ccgccgggct ggaggacgag ccggccaccg aggcgccgcg tcgagccgtg cagcgcaagg
     1321 cggcgtcggt gcgcagcgcg ccccgcgtgg atctcgccgc cctcgccgcg tcccggccgg
     1381 cactggcgga cggggctgcc ggtgcgccgg tgcccgatct gttccccgtg cgcagcttcg
     1441 cgccaccgcc accgcctccc ccaccaccgc ccacgccgac ggcgccgccg ttgcccttcc
     1501 gctacaccgg catgctggaa gagggcggcc gccacgccgc cttcctggag cagggtgagc
     1561 agatccgcgt ggtgcgggtc ggtgacaccc tcgacggacg ctaccgggtg acggcggtga
     1621 gccgcgcccg catcgacctg atctatctgc cgctcaacga accccagagc ctcgtgacgg
     1681 gggccatgcc atgacttcga gacgccttcc ccgactcctg cgccgcctgg cgcccctggc
     1741 gctgaccgtg ctgctcgcgg cctgcgccgg caacaaggcg ctggaggaga gccgccgctc
     1801 ctttgccacc cagagccccg acgtggccct gatcgacctg cgcagccgcg tcgaggccga
     1861 tccgcgcaac ctggagctgc gcgcctatta cctgcgccag cgggaggccc tgacgacccg
     1921 ccagctggcc atggccgacc aggcccgcgg cgcggggcgc ttcgaagaag cggagaacgc
     1981 cctggccctg gcgcgccagt tcgaccccaa caacccccgc gtggtggccg gtgtggacgc
     2041 catcgccgcc cagcgtcagc gcaaccagct ggcggcggag gccgaccggc gcctggccgc
     2101 cggcgacttt gcc
//
LOCUS       DAOQPA010000256         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_47306_length_2106_cov_14.486592, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000256 DAOQPA010000000
VERSION     DAOQPA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47306_length_2106_cov_14.486592"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..483
                     /locus_tag="PKX01_17580"
     CDS             85..483
                     /locus_tag="PKX01_17580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ78001.1"
                     /translation="MEIEYRHNFPLNPADVARVFDAAGLKRPTDNIPRIARMFAASNL
                     VISAWESGVLVGVCRALTDYSYCCYLSDLAVDRAYQKRGIGKALVRHVQDVIGDEVSL
                     VLLSAPDALSYYPSVGFCRAENAFLIKRAK"
     gene            852..1436
                     /locus_tag="PKX01_17585"
     CDS             852..1436
                     /locus_tag="PKX01_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ78002.1"
                     /translation="MFHQHFTTRTLRSAVVAGLAALAITAPALAFDFTGTKSLIAVTA
                     DGQRKTLGAVQFVPAKAGQATFKVVLQTEVFTDHFLSMREFKCLAGAKEVSCYVPYPY
                     PHPATVGPGRLVWLEHSLLFLYKSPAEFGARLTNGVYYEFREQGAALVGRPMAVDLNE
                     ISAPPADASVPPYGPAQRQEMDASTRWVRELVIE"
     gene            1499..2101
                     /locus_tag="PKX01_17590"
     CDS             1499..2101
                     /locus_tag="PKX01_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ78003.1"
                     /translation="MSGEGKRRTWMRWVLVAVVVLGAAAAFVAYRDHWPRSGGLVQAL
                     ANEARAANPDRATVAVILEAAFSETRANASRWSGVFWGFSFAAVALSAVAGLVLKLET
                     LPWSDRVRKDVGAILSVLSALIVTISTNGDFHRKWQANRLAAAQIEDLSYRFLRDPAT
                     DPRRHLAELGRIQLQRQLEIVGAKGAELPGAAEPCETKAE"
BASE COUNT          327 a          641 c          759 g          379 t
ORIGIN      
        1 ggaggacgct gacgggcggg ggtggcgggc ggccgcaggc catgatctag tgccaccatt
       61 gcgtcgaatg ctacattgca cctcatggaa attgaatacc gccacaactt tcccttgaac
      121 cctgcggacg tcgcccgggt gttcgatgcc gcggggctca agcggccgac cgataacatt
      181 ccgcgcatcg ccagaatgtt tgccgcgtcg aatctggtga tctccgcatg ggaaagtggt
      241 gttctggtcg gcgtgtgtcg tgcgctgacc gactacagtt attgttgcta tctgtcggat
      301 ctggcggtcg atcgggcgta tcagaagcgc ggtatcggca aggcgctggt ccggcacgtg
      361 caggacgtga ttggcgacga ggtgtcactc gtcctgttgt cggcgcccga cgccctgtcg
      421 tattacccgt ccgtgggatt ctgccgggcg gagaatgctt tcctcatcaa gagggccaag
      481 tgatgtccgg tcagcgggct ggaagggagc ggcgacatgc agtgcatcct gttttgccgg
      541 gacgttgccg cccggcgcgc ccccgatgca ggccccggcc tccgcgatga ccgtcccgtg
      601 cggcatatcc gtgcgtcaac ccctgaaatg ccgtcctgag tgcgtggcgt ttctctaaaa
      661 agattttcgt gcgagcaaac caggcattga cccgcggcaa tgcacctgaa ggcatccggg
      721 taccaggata ggggcgtggt cgacgcccga cgtgcttgct tcgcaaacac cgcagagtgg
      781 ccgccgtgcg ccagtgtgcg cacgtggccc ctcgaatgaa acgggttctt ttctggcgag
      841 gacacactcc catgttccac caacacttca ccacacgcac actgcgttcc gccgttgtcg
      901 caggcctcgc agcgctcgca ataaccgccc ccgcccttgc cttcgacttc accggcacca
      961 agtccctcat cgccgtcacc gccgatggtc agcgcaagac cctcggtgcc gtgcagttcg
     1021 ttccggcgaa ggcgggccag gcgacgttca aggtggtgct ccagacggag gtcttcacgg
     1081 accacttcct gtccatgcgg gagttcaagt gccttgcggg ggcgaaggag gtgagctgct
     1141 acgtgcccta tccctatccc catcccgcga cggtggggcc ggggcggctg gtgtggctgg
     1201 agcacagcct gctgtttctc tacaagagcc ctgcggagtt cggcgcgcgg ctcaccaacg
     1261 gggtgtatta cgagtttcgg gagcagggcg cggccctcgt cggccgtccg atggcggtgg
     1321 atctgaacga gatctcggcg ccgcccgcgg acgcctccgt gccgccctac gggccggcgc
     1381 agcgtcagga gatggacgcg tcgacgcggt gggtgcggga gctggtgatc gagtagcgtc
     1441 ggcggtggcg aacggcgtgc gtgggggatg atgtgtggcg gggtaggggg agggcggaat
     1501 gagcggggag ggcaagcgca ggacctggat gcggtgggtc ttggtcgccg tggtggtgct
     1561 gggcgccgcc gcggccttcg tggcctaccg ggatcactgg ccacgcagcg gagggctggt
     1621 gcaggcgctg gcgaacgagg ccagggccgc gaatccggac cgcgcgacgg tcgcggtcat
     1681 tctggaggcg gcgttcagcg agacgcgcgc caatgcgagc cgctggagcg gcgtgttctg
     1741 gggcttcagt tttgccgcgg tggccctgag cgcggtggcc gggctggtgt tgaagctgga
     1801 aacgctgccc tggtccgacc gggtccgcaa ggacgtgggg gcgatcctgt cggtgctatc
     1861 ggccttgatc gtcacgatct cgaccaatgg cgactttcac cgcaagtggc aggccaaccg
     1921 gctggcggcg gcgcagatcg aggacttgag ctatcgcttc ctgcgcgacc cggcgacgga
     1981 tccgcgccgc catctggcgg agctggggcg catccagctg cagcgccagc tggagatcgt
     2041 cggcgccaag ggcgcggagc tgcccggcgc ggcggagccg tgcgagacga aggcggaata
     2101 gacgcg
//
LOCUS       DAOQPA010000257         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_48529_length_2077_cov_18.032641, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000257 DAOQPA010000000
VERSION     DAOQPA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48529_length_2077_cov_18.032641"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /gene="dusB"
                     /locus_tag="PKX01_17595"
     CDS             <1..749
                     /gene="dusB"
                     /locus_tag="PKX01_17595"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMZ78004.1"
                     /translation="DINMGCPAKKVCNVMAGSALMQDEALVARILEAVVGAAGATPVT
                     LKFRTGWSKAHKNAATIARIAEASGVQLIAIHGRTRACQYTGDAEYDTIADVKTRVRI
                     PVIANGDITTPHKARFVLDYTGADGVMIGRAAQGRPWIFREIEHFLSTGEELPPPLVS
                     EIHQVCRDHLADLYAFYGEDTGVKVARKHISWYTKGLAGSAQFRRVMNTLPTVQEQLT
                     AMDEFFDRLADRDSRLVYVDPEDFPKELAA"
     gene            746..985
                     /locus_tag="PKX01_17600"
     CDS             746..985
                     /locus_tag="PKX01_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ78005.1"
                     /translation="MSRSSNDIAESVHRALETYFRDLDGEKPNALYDMVIRNVERPML
                     EFVLQQAGGNQTLCAEMLGINRNTLRRKLSEYQLL"
     gene            1038..>2077
                     /gene="purH"
                     /locus_tag="PKX01_17605"
     CDS             1038..>2077
                     /gene="purH"
                     /locus_tag="PKX01_17605"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMZ78006.1"
                     /translation="MKVTQALLSVSDKRGVVEFARELSALGIQLLSTGGTAKSLRDAG
                     LPVTDVSDYTGFPEMLDGRVKTLHPKVHGGILARRDLPEHLAKLEAHSIPRIDLVVVN
                     LYPFQATVAKPDCTLEDAIENIDIGGPTMVRAAAKNHGNEAGGVGIVTDPEDYAAIVA
                     ELQANAGALSYKTRFDLARKAFTHTARYDSAISNHLTALAEDGSKQAYPERFQLAFDK
                     VQDLRYGENPHQSAAFYKESDAPAGAIAAYTQVQGKELSYNNIADADAAWECVKAFDA
                     ATPACVIVKHANPCGVAVAASPLDAYKKAFSTDPTSAFGGIIAFNTVVDKAAAEAVSA
                     QFLEVLIAPAYT"
BASE COUNT          406 a          844 c          549 g          278 t
ORIGIN      
        1 tcgacatcaa catgggctgc ccggccaaga aggtctgcaa cgtcatggcc ggctccgcgc
       61 tgatgcagga cgaagccctc gtggcgcgca tcctcgaagc cgtcgtcggc gcggccggcg
      121 ccacgccggt caccctcaag ttccgcaccg gctggagcaa ggcccacaag aacgccgcca
      181 ccatcgcccg catcgccgaa gcctccggcg tgcagctcat cgccattcat ggccgcaccc
      241 gcgcgtgcca gtacaccggc gatgccgaat acgacaccat cgccgacgtg aagacccgtg
      301 tgcgcatccc ggtcatcgcc aacggcgaca tcacaacgcc ccacaaggcc aggttcgtcc
      361 tcgactacac cggcgccgac ggcgtcatga tcggccgcgc cgcccagggc cgtccctgga
      421 tcttccgcga gatcgaacac ttcctgagca ccggcgagga actcccgccg ccgctcgtca
      481 gcgaaatcca ccaggtctgc cgcgaccacc tcgccgacct ctacgccttc tacggcgaag
      541 acaccggcgt caaggtcgcc cgcaagcaca tcagctggta caccaagggt ctggccggct
      601 cggcgcagtt ccgccgggtc atgaacaccc tgccgacagt acaagaacaa ctcaccgcca
      661 tggacgaatt cttcgaccgg ctcgccgaca gagacagccg cctggtctac gtggacccgg
      721 aagactttcc caaggagctt gccgcatgag tcgcagcagc aacgacatcg ccgaatccgt
      781 ccatcgcgcc ctggagacct atttccgcga cctggacggc gagaagccca acgccctcta
      841 cgacatggtg atccgcaacg tcgagcgccc catgctcgaa ttcgtcctcc agcaggccgg
      901 cggcaaccag accctgtgcg ccgaaatgct cggcatcaac cgcaacaccc tgcgccgcaa
      961 gctctccgaa taccagctcc tgtaaccgat tcgcccatcc ccgccgcggc ccggcccgcg
     1021 gccttccaga gaagcccatg aaagtcaccc aagccctgct cagcgtgtcc gacaagcgcg
     1081 gcgtcgtcga attcgcccgc gaactctccg ccctcggcat ccagctcctg tccaccggcg
     1141 gcaccgccaa gtccctgcgc gacgccggcc tgcccgtcac cgacgtctcc gactacaccg
     1201 gcttcccgga aatgctcgat ggccgcgtca agaccctgca ccccaaggtg cacggcggca
     1261 tcctcgcccg gcgtgacctg cccgaacacc tggccaagct tgaagcgcac agcatcccgc
     1321 gcatcgacct ggtggtcgtg aacctctacc ccttccaggc caccgtcgcc aagcccgact
     1381 gcaccctcga agacgccatc gagaacatcg atatcggcgg ccccaccatg gtccgcgccg
     1441 ccgccaagaa ccacggcaac gaagccggcg gtgtcggcat cgtcaccgac ccggaagact
     1501 acgccgccat cgtcgccgag ctgcaggcca acgcgggcgc cctgtcctac aagacccgct
     1561 tcgacctggc gcgcaaggcc ttcacccaca ccgcccgcta cgacagcgcc atctccaacc
     1621 acctcaccgc cctggccgaa gacggcagca agcaggccta ccccgagcgc ttccagctcg
     1681 ccttcgacaa ggtgcaggac ctgcgctacg gtgagaaccc ccaccagagc gccgccttct
     1741 acaaagagtc cgacgccccc gccggcgcca tcgccgccta cacccaggtg cagggcaagg
     1801 aactctccta caacaacatc gccgacgccg acgccgcgtg ggaatgcgtc aaggccttcg
     1861 acgccgccac ccccgcctgc gtcatcgtca agcacgccaa cccctgtggc gtggccgtcg
     1921 ccgccagccc gctggacgcc tacaagaagg ccttctccac cgaccccacg tcagccttcg
     1981 gcggcatcat cgccttcaac accgtcgtcg acaaagccgc cgccgaagcc gtcagcgccc
     2041 agttcctcga agtgctgatc gcccccgcct acaccga
//
LOCUS       DAOQPA010000258         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_50595_length_2032_cov_22.555893, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000258 DAOQPA010000000
VERSION     DAOQPA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50595_length_2032_cov_22.555893"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKX01_17610"
     CDS             <1..308
                     /locus_tag="PKX01_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ78007.1"
                     /translation="ETQATASCTVGSHESRTFGTVLQGHQTIWANSGLLGTLNLLKSL
                     TWPSTLPVIALPSAFFGSQQVDFNQWAWFFTVLRTLVIATASIAAYRIIFVGGGQTT"
     gene            305..580
                     /locus_tag="PKX01_17615"
     CDS             305..580
                     /locus_tag="PKX01_17615"
                     /inference="COORDINATES: protein motif:HMM:NF022194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HMZ78008.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLGIWDSLLSVADSTLATIGTA
                     GLTLPVIPDQYAWVLGATGMSQALAIVASAMGTRFILQTIPFVRWGS"
     gene            660..1664
                     /locus_tag="PKX01_17620"
     CDS             660..1664
                     /locus_tag="PKX01_17620"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HMZ78009.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVKQGRRLYIAVDGIYLDRL
                     SLFTGIDLQTLEQQITIWKDSVEVLQAFPHVEPGSAVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRKLAFVGLSKKYQGKVRGNPED
                     HEVIRAFVGTYSPAIYAYYSSYASAAIREEKRSHTVFKSARVAIGIAAGLFALTLMVW
                     RPWSSLGSTKPTPAGSPSVPSIASGPAVQGSGSLLNPLGLAPSPAATPAPAKRTVRIL
                     GGAGMSKDRQGWRYLLDSGEILTAAQITGRYGLMVSEVYEEGSMRLIGEGVFYGPAGD
                     "
BASE COUNT          364 a          589 c          632 g          447 t
ORIGIN      
        1 aagagacgca agcgacggca tcatgcacag tggggtccca tgagagccgg acctttggca
       61 cggtcctcca agggcatcag accatctggg ccaacagtgg cttactcggc acattgaatc
      121 tcttgaagtc gctgacgtgg ccctcgacct tgccggtgat tgccttgccc tcggccttct
      181 ttggcagcca gcaagtcgat tttaaccaat gggcctggtt cttcaccgtg ctccgaacat
      241 tggtcatcgc cacagcctcc attgctgcct accgcatcat cttcgtcggt ggagggcaaa
      301 cgacatgacc gcgattctga ccctcatcta ttgctggcta caggagttct tcttctccct
      361 cacggattgg ggcctcggga tctgggattc cctgctctcc gtggcggaca gcacgctcgc
      421 caccattggc acggcagggc tcaccctgcc ggtgattccc gatcaatatg cgtgggtgct
      481 gggcgcgacg ggcatgagtc aggcgctggc catcgtggcg agcgccatgg gcactcggtt
      541 cattttgcaa accattccat tcgtccggtg gggctcatga accaactggt gatgggctgg
      601 gcctatgcct ggacggtgat tttagctctc tggatctggg tgtatctcag gagtcttcga
      661 tgatcgaact gtatgaaggg gtgccgggct cagggaaatc gtatcacgcg atctgcgaga
      721 agttcttgcc ctgggtgaaa caaggccgac ggctctatat cgccgtcgat gggatctatc
      781 tggatcggtt gtccctcttc actgggatcg accttcaaac gctcgaacaa cagatcacga
      841 tttggaagga ctccgtcgaa gtcctccagg cctttcccca tgtcgaacca ggctcagccg
      901 tcatcatcga cgaagcgcaa accgtgtttc gctccatgca gaaggtggag ccgggcctgc
      961 tccgctggct cgaaacgcat cggcattacg gcgtggatat cctgctcatg agtcaggatt
     1021 ttcggcagat gtcgcaaggc gtcacacggc tcatcgaagc cacagtgaag tttcggaagc
     1081 tggctttcgt gggcctgtcc aaaaagtatc agggcaaggt gcgtgggaat ccggaggacc
     1141 acgaagtgat ccgggctttc gtcggcacct actcgccggc catctacgcc tactattcga
     1201 gttacgcctc ggcagccatt cgagaagaga aacgcagtca taccgtcttc aagtcggcgc
     1261 gggtggcgat cggaatcgcc gctgggctgt ttgctctgac gctcatggtg tggcggccct
     1321 ggtcgtccct gggttccacg aagccgactc ccgccggatc accgtctgtg ccgtccatcg
     1381 cgtcggggcc agccgttcaa ggctcgggca gcctgctcaa tcccttgggc ctggctccgt
     1441 cacccgctgc gactcccgct ccagccaagc gaacggtgcg aatactcggc ggtgccggaa
     1501 tgagcaagga ccgccaaggt tggcgctacc tgctggatag cggcgagatc ctgacagcgg
     1561 cacagatcac cggacgctat ggcctcatgg tctcagaagt ctatgaagag ggatcgatgc
     1621 gactgattgg cgaaggggtg ttctatggac ctgccggcga ttgaggcgat gacctacttc
     1681 acggcgatct tctggctcgg cggctttgcc gtcgggctga tcatcaaact gattctgcct
     1741 cagcatcact gaggcgctcg accgccggcg agtcatccgg cggcgtgggt ggacggaacc
     1801 accaacagca caaggaggtg ccgtatgaag ggatggggtt gggtgaaagg gcttgggggg
     1861 ctgctcatgg cgttcgtgtt cgccttgggt gtcccggcgc tgtcgcaggc gcagttgttt
     1921 ccggtctcgg cggatgtggc cacggtgcgg gccgatctgc tcttgtgggc cacggctttg
     1981 attggcgtgg cgttggcgat ctacgccttt aagcgcgtcc gcgcaattgt gg
//
LOCUS       DAOQPA010000259         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_52332_length_1995_cov_15.700000, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000259 DAOQPA010000000
VERSION     DAOQPA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52332_length_1995_cov_15.700000"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PKX01_17625"
     CDS             complement(<1..113)
                     /locus_tag="PKX01_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ78010.1"
                     /translation="MFDKILIANRGEIACRVIKTARRLGIRTVAVYSEADAG"
     gene            complement(141..812)
                     /locus_tag="PKX01_17630"
     CDS             complement(141..812)
                     /locus_tag="PKX01_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HMZ78011.1"
                     /translation="MDWTPAGLAVAAEGLRPLLDGGGIPLDLASLTALAAGLGWASGL
                     RLYALVFVLGALGRFAGVSLPGGLAVLSHPLVLGLSGLLLLVEFGADKLPWLDSVWDA
                     VHTFIRIPAGAALAAAVMGDQGGAAQVALGLLGGTLAAGTHFAKAGTRAALNTSPEPV
                     SNVAASVGEDALFAGGLWTLLHHPLVFLAALALFAVVAGLVLRALWGVMKKVLRPRMR
                     PAAPR"
     gene            complement(820..1611)
                     /locus_tag="PKX01_17635"
     CDS             complement(820..1611)
                     /locus_tag="PKX01_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMZ78012.1"
                     /translation="MTYETLEIERAGAVATVWMNRPQVHNAFNETLIAELGAAWAALD
                     ADDAVRVVVLAGRGRSFSAGADLAWMQRAGAASVDDNLADAGRLAGMLRGLAELSKPT
                     VARVHGAALGGGMGLAAACDLCVAGSGAVFATSEVRLGLIPAAIGPYVIRALGERQAY
                     RYFLTAERIDARRAAALGLAHEAVEADALDTRVRDLCDALLLGGPQAQRAAKALIRAV
                     AKRPVDDALVADTAHRIATLRATPEAREGLDAFLAKRPAAWVPGA"
     gene            complement(1622..>1995)
                     /locus_tag="PKX01_17640"
     CDS             complement(1622..>1995)
                     /locus_tag="PKX01_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ78013.1"
                     /translation="IGGSFGAGNYGMCGRAYSPRFLWMWPNSRISVMGGEQAASVLAT
                     VRRDGIEARGGRWSRDEEEAFKSPIRQQYEDQGHPYYATARLWDDGVIDPAQTRRILG
                     LSLSASLNTPILPTRFGVFRM"
BASE COUNT          301 a          757 c          689 g          248 t
ORIGIN      
        1 ccggcgtcgg cttccgaata caccgccacc gtccggatgc ccaggcggcg ggcggtcttg
       61 atcacccggc aggcgatctc gccccggttg gcgatcagga tcttgtcgaa catgctgtct
      121 cctctccagt tgtccttttg ttatcggggt gccgccgggc gcatccgcgg ccgcagcacc
      181 tttttcatga cgccccacag ggcccgcagc accagcccgg cgacaacggc gaagagcgcc
      241 agcgccgcca ggaacaccag cgggtgatgc agcagcgtcc acaggcctcc ggcgaacagg
      301 gcgtcctcgc ccaccgacgc ggccacattg ctcaccggct cgggcgaggt gttgagggcc
      361 gcccgcgtgc ccgccttggc gaaatgggtg cccgccgcca gcgtgccgcc gagcaggccc
      421 agcgccacct gggcagcacc gccctggtcg cccatcaccg ctgcagcaag ggccgcaccc
      481 gccggtatgc ggatgaaggt atgcaccgcg tcccatacgg aatcgagcca gggcagcttg
      541 tcggcgccga actccaccag cagcagcagg ccggacagtc ccagcaccag gggatgactc
      601 agcaccgcca gaccgccggg caggctcacc ccggcaaaac gccccagcgc ccccagaacg
      661 aagaccagcg cgtacaggcg caggccgctc gcccagccca ggccggccgc cagcgccgtc
      721 agggaagcca ggtccagcgg gatgccgccc ccgtcgagca ggggacgcag cccttccgcc
      781 gccaccgcga gccccgccgg cgtccagtcc atcgcgacct caggcgcccg gcacccaggc
      841 ggccggccgt ttggcgagga aggcgtcgag cccttcccgc gcttccggcg tggcccgcag
      901 ggtggcgatg cgatgggcgg tgtcggccac cagggcgtcg tccaccggcc gcttcgccac
      961 ggcgcggatc agcgccttgg ccgcccgttg ggcctggggc ccgccgagca gcagggcgtc
     1021 gcacaggtcc cggacacggg tatccagcgc atccgcctcc accgcctcgt gggcgaggcc
     1081 cagcgccgcc gcccgccgcg cgtcgatgcg ttcggccgtg aggaagtagc ggtaggcctg
     1141 gcgctcgccg agagcgcgga tcacgtaggg cccgatggcc gccgggatca ggccgaggcg
     1201 cacctccgag gtggcgaaca ccgccccgga acccgccacg cacaggtcgc aggccgcggc
     1261 gaggcccatc ccgccgccca gggccgcgcc gtgcacccgc gccaccgtgg gcttggacag
     1321 ctccgccaac ccgcgcagca tgcccgccag gcggccggca tcggccaggt tgtcgtccac
     1381 cgacgcagcg ccggcacgct gcatccaggc cagatcggcg ccggcggaaa aactccgtcc
     1441 gcgcccggcc aataccacca cacgcaccgc gtcgtccgcg tccagggcgg cccaggcggc
     1501 gccgagttcg gcgatcaggg tctcgttgaa ggcgttgtgc acctgggggc ggttcatcca
     1561 cacggtggcg acggcgcctg cgcgttcgat ttcaagggtc tcgtaggtca tggcgcccgc
     1621 ctcacatccg gaacacgccg aacctggtcg gcaggatcgg cgtgttgagg ctggccgaca
     1681 gggacaagcc gaggatgcgg cgggtctggg cggggtcgat gacgccgtcg tcccacaggc
     1741 gcgcggtggc gtagtaggga tggccctggt cctcgtactg ctggcggatc ggcgacttga
     1801 aggcttcttc ctcgtcgcgg ctccagcgac cgcccctggc ctcgatgcca tcccggcgca
     1861 cggtggcgag gacgctggcg gcctgctcgc cgcccatcac cgagatacgg ctgttgggcc
     1921 acatccacag aaagcgcggg ctgtaggcac ggccgcacat gccgtagttg ccggcgccga
     1981 agctgccgcc gatga
//
LOCUS       DAOQPA010000260         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_53868_length_1965_cov_21.687435, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000260 DAOQPA010000000
VERSION     DAOQPA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53868_length_1965_cov_21.687435"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PKX01_17645"
     CDS             <1..1053
                     /locus_tag="PKX01_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ78014.1"
                     /translation="AGAAQRILPKSKQKKLIGKTEIEEIVAKIARIPPRNVSNDDKAA
                     LRTLERDLKNVVFGQDAAIEALAKAIKMSRSGLGNPQKPIGSFLFSGPTGVGKTEVAR
                     QLAYTLGIELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEQITKKPHCVLLLD
                     EIEKAHPDIYNILLQVMDHGTLTDNNGRQADFRNAIIIMTTNAGAETMQKSVIGFSAK
                     REVGDEMAEIKRMFSPEFRNRLDATISFKSLDHDIVMKVVDKFLMQLEAQLHEKKVDA
                     HFTDRLKEWLGQKGFDPLMGARPMARLIQDTLRASLADELLFGRLAGGGHVTIDLDDN
                     GKVALQFEEEADVATV"
     gene            1134..1613
                     /gene="rraA"
                     /locus_tag="PKX01_17650"
     CDS             1134..1613
                     /gene="rraA"
                     /locus_tag="PKX01_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HMZ78015.1"
                     /translation="MTFQTTDLCDANEGKVRVVAPMFRSFGGKTRFAGPISTLKVFED
                     NSLVRSALETAGAGRVLVVDGGGSMRCAMVGDQLAALGVKNGWAGIVVYGCIRDSGPI
                     AGMDIGVFALGTHPQKSVKKGAGDQDIPVTFGGVTFVPGHWLYADEDGVIVADAPLV"
BASE COUNT          394 a          655 c          567 g          349 t
ORIGIN      
        1 gccggcgccg cccagcgcat cctgcccaag tccaagcaaa agaagctcat cggcaagacc
       61 gaaatcgagg agatcgtcgc caagatcgcg cgcattccgc cgcgcaacgt ctccaacgac
      121 gacaaggcgg ccctgcgcac cctcgaacgg gatctcaaga acgtggtctt cggccaggac
      181 gcggccatcg aggccctcgc caaggccatc aagatgtccc gctcgggcct cggcaatccg
      241 cagaagccca tcggttcctt cctgttcagc ggccccaccg gcgtcggcaa gaccgaggtc
      301 gcccgccagc tcgcctacac cctgggcatc gagctggtgc gcttcgacat gtctgaatac
      361 atggagcgtc acgcggtgtc ccgcctcatc ggcgcgccgc cgggctacgt gggctttgac
      421 cagggcggcc tgctcaccga gcagatcacc aagaagcccc actgcgtgct gctgctggat
      481 gaaatcgaga aggcccaccc ggacatctac aacatcctgc tgcaggtgat ggaccacggc
      541 accctgacgg acaacaacgg gcgccaggcc gacttccgca acgccatcat catcatgacg
      601 accaatgcgg gcgccgagac catgcagaag tcggtcatcg gcttctcggc caagcgcgag
      661 gtcggcgacg agatggcaga gatcaagcgg atgttctcgc cggaattccg caaccggctg
      721 gacgccacca tctccttcaa gtccctcgac cacgacatcg tgatgaaggt cgtggacaag
      781 ttcctgatgc agctcgaagc ccagctccac gagaagaagg tggacgccca cttcaccgac
      841 cgcctcaagg aatggctcgg ccagaagggc ttcgatccgc tcatgggcgc acggccgatg
      901 gcgcgcctca tccaggacac cctgcgcgcc agcctggccg acgagttgct cttcgggcga
      961 ctggccggtg gcgggcacgt caccatcgac ctggacgaca atggcaaggt ggccctgcag
     1021 ttcgaggaag aggccgacgt ggccacagtc tgatccggac ggcctacaat cgcgggcggc
     1081 ttcggccgcc ctttttcgtt gacctgttta agaccctccc ccaggaaacc gacatgactt
     1141 tccaaaccac cgatctgtgc gacgcgaatg aaggcaaggt ccgcgtcgtc gcccccatgt
     1201 tccgcagctt cggcggcaag acccgcttcg ccggccccat cagcaccctc aaggtgttcg
     1261 aggacaactc cctggtccgc tccgcgctgg agaccgccgg cgcgggccgc gtgctggtgg
     1321 tggacggcgg cggctcgatg cgctgcgcca tggtgggcga tcagctcgcc gcactgggcg
     1381 tgaagaacgg ctgggccggc atcgtggtat acggctgcat ccgcgactcg ggccccatcg
     1441 ctggcatgga catcggcgtg ttcgcgctgg gcacccatcc gcagaagagc gtcaagaagg
     1501 gcgcgggcga ccaggatatt ccggtcacct tcggcggcgt cacctttgta ccgggacact
     1561 ggctgtacgc ggatgaggat ggcgtgatcg tcgccgacgc gccgcttgtg taagacttca
     1621 cacacaacgc cgtgcagcgc agcgactttt tatttcacct gcgctaacgg cgtttcacaa
     1681 tacaaaaaat acagagattt tcattgattt tacagggaaa tttatttctt atatcttata
     1741 taagacctgt tgctgccgtg caaaatcccc ccctatactc tcgctcactg aaacgcacat
     1801 ttcacagaga gtttcccccc tcctgtcaga agtgcgacag cgcggcttcg taaccggtca
     1861 caggttgccc gctcccagtt ttccctcccc ctcaacgttt cttaaggatg tcgttatgtc
     1921 tctgactcgc gaacagcaaa tcgccgccct ggaaaaggat tgggc
//
LOCUS       DAOQPA010000261         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_56519_length_1918_cov_19.638218, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000261 DAOQPA010000000
VERSION     DAOQPA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56519_length_1918_cov_19.638218"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKX01_17655"
     CDS             complement(<1..269)
                     /locus_tag="PKX01_17655"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584312.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HMZ78016.1"
                     /translation="MDSDSRKQRGAAKTARIPIKIVPAEKLRKPEWIRIKLGAGAEIE
                     RFNEIKDTLREHKLHTVCEEASCPNIHECFGKGTATFMIMGDICTR"
     gene            complement(289..900)
                     /gene="lipB"
                     /locus_tag="PKX01_17660"
     CDS             complement(289..900)
                     /gene="lipB"
                     /locus_tag="PKX01_17660"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HMZ78017.1"
                     /translation="MIVRRLGLVPYEPTWRAMQDFTATRDAATPDELWLLQHPPVYTL
                     GQAGKPEHLIRPTEIPLVKIDRGGQITYHGPGQVVGYLLLDLHRRHLKVREMVHQLEQ
                     ALIDCIADYGLDARRKDGAPGVYIDGAKIAALGLRVRNGCSYHGLSLNVDMDLTPFTW
                     INPCGYSGLQTIQLKDFGVTEAPDTVAERLLGHLQRHFPDPAA"
     gene            complement(897..1166)
                     /locus_tag="PKX01_17665"
     CDS             complement(897..1166)
                     /locus_tag="PKX01_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HMZ78018.1"
                     /translation="MSEERQTLLEFPCAFPIKIMGARVDAFAQTVLDVVLRHDPGFDA
                     AAMEMRPSSKGTYLAITCTVTAISQTQLDNLYRELSSHPMVKVVL"
     gene            complement(1310..>1918)
                     /locus_tag="PKX01_17670"
     CDS             complement(1310..>1918)
                     /locus_tag="PKX01_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HMZ78019.1"
                     /translation="ASAIIRDFPEFYPIYSTKEYTYNKITQPNRNRLLFLDPTVDGVK
                     TGHTESAGYCLIASAKRNERRLISVVVGTTSDSVRAAESQKLLNWGYLAYDAVKVYSA
                     KQPVQEFRLWKGAANSIKVGFLKDLVLSLPKGDADKLKMTVVSQEPLIAPVTQGQPLA
                     TLQLTLDGKPVADYPLVALDDVPQAGWFGRVWDAIRLWFKSL"
BASE COUNT          279 a          536 c          758 g          345 t
ORIGIN      
        1 cgggtgcaga tgtcgcccat gatcatgaag gtggccgtgc ccttgccgaa gcattcgtgg
       61 atgttggggc aggaggcttc ctcgcagacg gtgtgcagct tgtgctcgcg cagggtgtcc
      121 ttgatctcgt tgaagcgctc gatctcggcg ccggcgccga gcttgatgcg gatccactcg
      181 ggcttgcgca gcttttcggc gggcacgatc ttgatgggaa tgcgggcggt cttggccgca
      241 ccgcgctgct tgcggctgtc ggaatccatg gggctgcgtc ctctggtgtc aggcagcggg
      301 atcgggaaaa tgccgctgca ggtggccgag caggcgctcg gcgacggtgt cgggggcttc
      361 ggtgacgccg aagtctttga gctggatggt ctggagccca ctatagccgc agggattgat
      421 ccaggtaaat ggtgtcaaat ccatatcgac attgagggac aaaccgtggt aggagcagcc
      481 gttcctgacc cgcaggccga gggcggcgat cttggcgccg tcgatgtaca cgcccggcgc
      541 gccgtccttg cggcgggcgt cgaggccgta gtcggcgatg cagtcgatga gggcctgttc
      601 gagctggtgc accatctcgc gcaccttgag gtggcggcgg tgcaggtcga ggagcaggta
      661 gcccaccacc tggccggggc cgtggtaggt gatctggccg ccccggtcga tcttgaccag
      721 ggggatctcc gtggggcgga tcaggtgttc gggcttgccg gcctggccga gggtgtagac
      781 cggcgggtgc tggagcagcc acagctcgtc cggcgtggcg gcgtcgcggg tggcggtgaa
      841 gtcctgcatg gcccgccagg tgggctcgta ggggaccagg ccgaggcgtc ggacgatcac
      901 aggacgacct tcaccatggg atgggacgac agctcgcggt agaggttgtc gagctgggtc
      961 tgggagatgg cggtcaccgt gcaggtgatg gccaggtagg tgcccttgga ggacgggcgc
     1021 atctccatgg cggcggcgtc gaagccgggg tcgtggcgca ggacgacgtc cagcaccgtc
     1081 tgggcgaagg catccacccg cgcgcccatg atcttgatgg ggaaggcgca ggggaattcg
     1141 agcagggtct gacgttcttc ggacatgggc gcgggccggg tgcggggtcg ggatctttcc
     1201 aagatgggac cgccgcggcg aaagttcaag ggcgggctgc gtgcaccgcg tcaggcggcc
     1261 cgcggtccgc cccgggccga ccgggcgcgg acccggccgg cggggggatt cacaggctct
     1321 tgaaccacag gcggatggcg tcccagacgc ggccgaacca gccggcctgg ggcacgtcgt
     1381 cgagggccac cagcgggtag tcggcgacgg gcttgccgtc cagggtgagc tgcagggtgg
     1441 ccaggggctg gccctgggtg accggcgcga tcaggggttc ctggctgacc acggtcatct
     1501 tcagcttgtc ggcgtcgccc ttgggcaggg acagcaccag gtccttgagg aagccgacct
     1561 tgatgctgtt ggcggcgccc ttccacaggc ggaattcctg caccggctgc ttggcggaat
     1621 agaccttgac cgcgtcgtag gccaggtagc cccagttgag gagcttctgg gattcggcgg
     1681 cgcgcacgct gtcgctggtg gtgcccacca ccacggagat gaggcggcgc tcgttgcgct
     1741 tggcggaggc gatcaggcag tagccggcgc tctcggtgtg gccggtcttg acgccgtcca
     1801 cggtggggtc gaggaagagc aggcggttgc ggttgggctg ggtgatcttg ttgtaggtgt
     1861 attccttggt ggaatagatc gggtagaact cggggaagtc gcggatgatg gcgctggc
//
LOCUS       DAOQPA010000262         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_57361_length_1903_cov_22.904762, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000262 DAOQPA010000000
VERSION     DAOQPA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57361_length_1903_cov_22.904762"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1903)
                     /locus_tag="PKX01_17675"
     CDS             complement(5..>1903)
                     /locus_tag="PKX01_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HMZ78020.1"
                     /translation="HYLADEEIFGTVEYHYEILAKRLRELSFLNNGVKIRLVDQRTGK
                     EEDFAFAGGVSGFVDYINRAKTVLHPTVFHASGESLQNGVPITVEVAMQWNDSYAEQV
                     LCFTNNIPQADGGTHLTGLRAAMTRVINKYIEENEIAKKAKVDITGDDMREGLACVLS
                     VKMPDPKFASQTKMKLVSGEARPAVEEVVAQKLADFLLERPLDAKTICGKIVEASRAR
                     EAARRAREMTRRKGVLDGVGLPGKLADCQEKDPALCEIYIVEGDSAGGSAKQGRDRKF
                     QAILPLRGKVLNVERARFDKLIGSEQIATLITALGTGIGSDDFKPEKLRYHRIIIMTD
                     ADVDGAHIRTLLLTFFYRQMRELVERGHIYIAQPPLYKIKHGKTELYIKDDTELNAHL
                     LKLGLDGAALTPRDGAEPIQAEALGGLARTYLLAEAVIQRLADWIDATVLHALLAHDL
                     SLSLDDEAAARDSAARLQAAVGAAVAIEARHDETVEGWTLQLARMHHGNRKLTRIDAD
                     FLQSGDWRIIRQAAEAISGLIGPGALMRRGEKQQAVTSFADAIRWMLADVERGLSKQR
                     YKGLGEMNPEQLWETTMDPAVRRLLKVQIDDAIAADEIFTTLMGDNVEPRRAFIEENA
                     LFARNIDV"
BASE COUNT          249 a          505 c          773 g          376 t
ORIGIN      
        1 accctcacac gtcaatattc cgcgcaaaca gcgcgttctc ctcgatgaac gcccggcggg
       61 gttcgacgtt gtcgcccatg agggtggtga agatctcgtc ggcggcgatg gcgtcgtcga
      121 tctggacctt gaggaggcgg cggacggcgg ggtccatggt ggtttcccag agctgctcgg
      181 ggttcatttc gccgaggcct ttgtagcgct gtttggagag gccgcgctcc acgtcggcga
      241 gcatccagcg gatggcgtcg gcgaagctgg tgacggcctg ctgcttttcg ccgcggcgca
      301 tgagggcgcc ggggccgatg aggccggaga tggcttcggc ggcctggcgg atgatgcgcc
      361 agtcgccgga ctggaggaag tcggcgtcga tgcgggtgag cttgcggttg ccgtggtgca
      421 tgcgggcgag ttggagggtc cagccttcga cggtttcgtc gtggcgggct tcgatggcga
      481 cggcggcgcc gacggcggcc tgcaggcggg cggcggagtc gcgggcggcg gcttcgtcgt
      541 cgagggacag gctgaggtcg tgggcgagga gggcgtgcag gacggtggcg tcgatccagt
      601 cggcgaggcg ctggatgacg gcttcggcga gcaggtaggt gcgggcgagg ccgccgaggg
      661 cttcggcctg gataggctcg gcgccgtcac ggggggtgag ggcggcgccg tcgaggccga
      721 gcttgaggag gtgggcgttg agctcggtgt cgtccttgat gtagagctcg gtcttgccgt
      781 gcttgatctt gtagaggggc ggctgggcga tgtagatgtg gccgcgctcg acgagttcgc
      841 gcatctgccg gtagaagaag gtgaggagca gggtgcggat gtgggcgccg tccacgtccg
      901 cgtcggtcat gatgatgatg cggtggtagc gaagcttttc gggcttgaag tcgtcgctgc
      961 cgatgccggt gcccagcgcg gtgatgaggg tggcgatctg ctcggagccg atgagcttgt
     1021 cgaagcgggc gcgctcgacg ttgaggacct tgccgcgcag gggcaggatc gcctggaact
     1081 tgcggtcgcg gccctgcttg gcggagccgc cggcggagtc gccctcgacg atgtagatct
     1141 cgcacagggc ggggtctttt tcctggcagt cggcgagctt gccgggcagg ccgacgccgt
     1201 cgaggacgcc cttgcggcgg gtcatctcgc gggcgcggcg ggcggcttcg cgggcgcggg
     1261 aggcttcgac gatcttgccg cagatggtct tggcgtcgag ggggcgttcg aggaggaagt
     1321 cggcgagctt ctgggcgacg acttcttcga ccgcgggccg ggcttcgccg gagaccagct
     1381 tcatcttggt ctgggaggcg aacttggggt cgggcatctt gaccgagagc acgcaggcga
     1441 ggccttcgcg catgtcgtca ccggtgatgt cgaccttggc cttcttggcg atctcgtttt
     1501 cttcgatgta cttgttgatg acgcgggtca tcgccgcgcg caggccggtg aggtgggtgc
     1561 cgccgtcggc ctgcgggatg ttgttggtga agcacagcac ctgttcggcg tagctgtcgt
     1621 tccactgcat ggccacttcg acggtgatgg gcacgccgtt ctggagggat tcgccgctgg
     1681 cgtggaagac ggtggggtgg agcacggtct tggcgcggtt gatgtagtcc acgaagccgg
     1741 agacgccacc ggcgaaggcg aagtcttctt ccttgccggt gcgctggtcc acgaggcgga
     1801 tcttgacgcc gttgttgagg aaggacagtt cgcgcaggcg cttggcgagg atttcgtagt
     1861 ggtattcgac ggtgccgaag atctcttcgt cggcgaggta gtg
//
LOCUS       DAOQPA010000263         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_63533_length_1803_cov_14.576659, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000263 DAOQPA010000000
VERSION     DAOQPA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63533_length_1803_cov_14.576659"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKX01_17680"
     CDS             complement(<1..361)
                     /locus_tag="PKX01_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMZ78021.1"
                     /translation="MTRFVPFATSLALLAFATAAPVQAEDFTVSSPSFSAAAPLPERH
                     VLDSFGCHGQNLSPALRWDHPPAGTRSFAVTLYDPDAPTGSGWWHWVVVDIPANQRAL
                     PEGAGNAGRPGLPAGALQ"
     gene            complement(506..1315)
                     /locus_tag="PKX01_17685"
     CDS             complement(506..1315)
                     /locus_tag="PKX01_17685"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ78022.1"
                     /translation="MDAAHGARVVATQAYTLKTLTIRDDAFIAVLGGRKTLHAPHARL
                     SADVQQGVMLARGTQWDVTNDPAGRREYAALAIAFPAELIRECHALEAAAGLPEVDAA
                     QVLAVDEELLAAVQRTLPPVAARPLAGALLRHRVLEVLILLAQRGHRFAPAESLPWPE
                     RVRRLVAQRLHADWDVATLATAFHVSESTLRRRLEGSGTTLAALVRDIRLETALGLLQ
                     TTRLPVGEVAARCGWKSHSRFTVAFQERWGVPPSVVRARLKDDAQDLTETA"
     gene            complement(1438..>1803)
                     /locus_tag="PKX01_17690"
     CDS             complement(1438..>1803)
                     /locus_tag="PKX01_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ78023.1"
                     /translation="IYVQDMARARAFYENVLGTTLVRMPAPTPEMDLDMLFFPMDKDT
                     GMNTYGSGGMLVRMEGMASGGGGTLVYFACEDCAVQAARAAEFGGRLCKEKTSIGEHG
                     FFSLAQDSEGNMIGFHSMK"
BASE COUNT          256 a          651 c          665 g          231 t
ORIGIN      
        1 cctgcagcgc gcccgccggc aggccggggc ggccggcgtt gccggcgcct tcgggcaacg
       61 cgcgctggtt ggcggggatg tccaccacca cccagtgcca ccagcccgag ccggtgggcg
      121 cgtccgggtc gtagagggtc accgcgaagc tgcgggtgcc ggcgggcggg tggtcccagc
      181 gcagggcggg ggaaaggttc tggccgtggc agccgaagct gtccagcacg tggcgctccg
      241 gcaggggcgc cgcagcggaa aagctcgggc tgctcaccgt gaagtcctcg gcctgcaccg
      301 gcgcggcggt ggcgaaggcg agcagggcga gggatgtggc gaaggggacg aagcgggtca
      361 tgtcgggcac tctccagggc agacgggccg ccggatgaac ggcggcgggc gggtcgggcg
      421 gcttcgggag ggcggtcggg ccccgcaggg cgcccaggcc ggccaaagcc gctgactgca
      481 tcgtgccggg ccgcgccgcg gcgcgctaag ccgtctccgt cagatcctgt gcgtcgtctt
      541 tcaggcgggc gcgcaccacc gagggcggca cgccccagcg ttcctgaaag gccaccgtga
      601 agcggctgtg ggacttccag ccgcagcgcg ccgccacctc gcccaccggc aggcgggtgg
      661 tctggagcag gcccagcgcg gtctccaggc ggatgtcccg gaccagcgcg gcgagggtcg
      721 tcccgctccc ttccaggcgc cgccgcaggg tcgattcgct cacatggaag gccgtcgcca
      781 gggtcgccac atcccagtcc gcgtggagcc gctgcgccac caggcggcgg acccgctccg
      841 gccagggcag ggattcggcc ggcgcaaagc ggtggccccg ctgggcgagg aggatcagca
      901 cctccagcac ccggtgacgc agcagggcgc cggccagcgg gcgggccgcc accggcggca
      961 gggtgcgctg cacggcggcg aggagttctt catccaccgc cagcacctgg gccgcgtcca
     1021 cctccggcag gccggcggcg gcctccaggg cgtggcattc gcggatcagc tcggcaggaa
     1081 aggcaatggc cagcgccgcg tattcgcgcc ggccggcggg atcgttggtc acgtcccact
     1141 gggtgccgcg ggccagcatc accccctgct gcacgtccgc cgacaggcgc gcgtggggcg
     1201 catgcagggt cttgcgcccg cccagcacgg caatgaaggc gtcgtcccgg atcgtcagcg
     1261 tcttgagcgt gtaagcctgc gtcgccacca cgcgcgcgcc gtgcgctgca tccatcggag
     1321 gggctcctgg ggtggaaaag gcccgatcct acaccggcgc cgacagtcct cgcccgccgc
     1381 gatccgccac cggagccgcg cgctcggcga gccccggacg tccggcccct gcggcgctca
     1441 tttcatcgag tggaagccga tcatgttgcc ttccgaatcc tgggcgaggg aaaagaagcc
     1501 gtgctcgccg atggacgtct tctccttgca caggcgtccg ccgaactcgg ccgcgcgggc
     1561 ggcctgcacc gcgcagtcct cgcaggcgaa atacaccagc gtcccgccgc cgcccgaggc
     1621 catgccctcc atccggacca gcatgccgcc ggaaccgtag gtgttcatcc ccgtgtcctt
     1681 gtccatcgga aagaacagca tgtccagatc catctccggc gtcggcgcag gcatcctgac
     1741 cagcgtcgtg cccaagacgt tctcgtagaa cgcgcgggcg cgcgccatgt cctgcacgta
     1801 gat
//
LOCUS       DAOQPA010000264         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_65248_length_1778_cov_11.640743, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000264 DAOQPA010000000
VERSION     DAOQPA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65248_length_1778_cov_11.640743"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKX01_17695"
     CDS             <1..704
                     /locus_tag="PKX01_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ78024.1"
                     /translation="GVQMVWIPFWAAGVINGLGHFWGYRNYNCADASTNLIPWGILIG
                     GEELHNNHHSFATSAKLSARWYEFDIGWVYIRSLEILGLARVKKVIPTPRFGVAKARA
                     DFDMLQAVITHRYDVMTRYVQSLGQIAKDEFEALKAQSGGKLNIKDVRRWLTADEAQL
                     GADERQRLATLLKDNARLQTVVAMRQELTALWARSTATREQLLQQLQDWIARAEGSGI
                     RQLEDFSLRLRRYAA"
     gene            854..1411
                     /locus_tag="PKX01_17700"
     CDS             854..1411
                     /locus_tag="PKX01_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HMZ78025.1"
                     /translation="MILLNKPYGVLCQFTDEAGRATLADYVPVPGVYAAGRLDADSEG
                     LLLLTDDGALQARIADPRHKLPKIYLAQVEGVPDVTALESLRRGVDLGDFVTRPCSAR
                     LVPEPDWLWPRDPPIRERKAIPVSWLEIVLREGKNRQVRRMTAKVGHPTLRLIRVAIG
                     PWTLGNLTPGAWRDDTIREKSVKNT"
     gene            complement(1493..>1778)
                     /locus_tag="PKX01_17705"
     CDS             complement(1493..>1778)
                     /locus_tag="PKX01_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ78026.1"
                     /translation="SSAVYMAGLATAQAVVALRGHHWVAVGWGLGFVVFVLATWLSSD
                     DLFRRIEIGLVASSVSAFVVFAVVLRAGRRRRRRWSPRTGRPPGRTTASS"
BASE COUNT          309 a          713 c          512 g          244 t
ORIGIN      
        1 ggggtgtgca gatggtgtgg atccccttct gggccgccgg cgtcatcaac ggcctggggc
       61 acttctgggg ctaccgcaac tacaactgtg ccgacgcctc caccaacctc atcccgtggg
      121 gcatcctcat cggcggcgag gaactgcaca acaaccacca ctcctttgcc acctccgcca
      181 agctgtcggc ccgctggtac gagttcgaca tcggctgggt gtacatccgc agcctcgaga
      241 tcctcggcct cgcccgcgtc aagaaggtca tccccacgcc gcgcttcggc gtcgccaagg
      301 cccgcgccga cttcgacatg ctgcaggccg tcatcactca ccgctacgac gtgatgaccc
      361 gctacgtgca gtccctcggc cagatcgcca aggacgagtt cgaagccctc aaggcccagt
      421 ctggcggcaa gctcaacatc aaggacgtgc gccgctggct caccgccgac gaagcccagc
      481 tcggcgccga cgagcgccag cgcctcgcca ccctgctcaa ggacaatgcc cgcctgcaga
      541 ccgtggtcgc catgcgccag gagctcaccg ccctgtgggc ccgctccacc gccacccgcg
      601 aacagctcct gcaacagctc caggactgga tcgcccgcgc cgaaggcagc ggcatccggc
      661 aactcgaaga cttctccctg cgcctgcgcc gctacgccgc gtgagccgtc cgcccccgcg
      721 cagacctgca caaacccgcc gggatggcgg gtttgtcgct tctgagggac gccgcaacgc
      781 cccccaggcc gccccggccc gcccaggcgc ttctccccgc gcgccgcgcg cggccccgcc
      841 ttccggcggc cgcctgatcc tcctcaacaa gccctacggc gtgctgtgcc agttcaccga
      901 tgaagccggc cgcgccaccc tcgccgacta cgtcccggta cccggcgtat atgccgccgg
      961 ccgcctcgac gccgacagcg aaggcctgct cctcctcacc gacgacggcg ccctgcaggc
     1021 acgcatcgcc gacccccgcc acaagctgcc caaaatctat ctcgcccagg tggaaggtgt
     1081 ccccgacgtc accgcgctcg aatccctgcg ccgtggcgtc gacctgggcg actttgtcac
     1141 ccgcccgtgc agcgcccgcc tcgtgcccga gcccgactgg ctgtggcccc gcgacccgcc
     1201 catccgcgaa cgcaaagcca tccccgtgag ctggctggaa atcgtcctgc gcgaaggcaa
     1261 gaaccgccag gtgcgccgca tgaccgccaa ggtcggacat cccacgctgc gcctcatccg
     1321 cgtcgccatc ggcccgtgga cactgggaaa cctcacgccc ggtgcatggc gcgatgacac
     1381 gattcgggaa aaatctgtta aaaacacctg accggtacgg cctggcccga tccgtcaggg
     1441 agggagcgaa gcccatccgt tctccatact cataaatctg cccaaggaat catcatgaag
     1501 aagctgttgt ccgccctggt ggccggcctg ttcgcggcga ccaccgccgt ctccgccgcc
     1561 gacctgcccg cagcaccacc gcgaacacga cgaacgcgct gacgctcgac gccacgagac
     1621 cgatctcgat gcggcggaac aggtcgtcgc tcgacagcca cgtggcgagc acgaagacca
     1681 cgaaaccgag gccccacccg accgccaccc agtggtggcc gcgcagggcg acgaccgcct
     1741 gcgccgtggc caggcccgcc atgtagacgg cgctcgac
//
LOCUS       DAOQPA010000265         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_66955_length_1754_cov_23.214832, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000265 DAOQPA010000000
VERSION     DAOQPA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66955_length_1754_cov_23.214832"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKX01_17710"
     CDS             complement(<1..476)
                     /locus_tag="PKX01_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMZ78027.1"
                     /translation="MFYREAGQFKATYAEDAQIFPIRQDRVGFWLMMLVFGVAVPVIA
                     DQYWLKAILVPVLIFSLAAIGLNILTGYAGQLSLGSAAFMAVGAFAAYNFILRVEGMP
                     FLVALVLAGLTAAAVGIVFGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNNSS"
     gene            complement(562..1488)
                     /locus_tag="PKX01_17715"
     CDS             complement(562..1488)
                     /locus_tag="PKX01_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMZ78028.1"
                     /translation="MQFFLEVLIGGLLSGVMYALVALGFVLIYKASGVFNFAQGAMVF
                     FAALTFVGFQELLPAHLGLAADGSAVFWLSFLLAFGVMVVLGIATERIVLRPLVNQPH
                     ITLFMATIGLTYVVEGLAQMTWGANVRGLDLGIVDEPIAWLMDTYEIGVSKFDLFAAG
                     VAASLVTLLALLFQYTKVGRALRAVADDHQAALSIGIPLQTIWRVVWAVAGFVALVAG
                     LIWGARNGVQFALTFTALKALPVLILGGFTSVPGAIVGGLIIGASEKLAEVYVGPLVG
                     GGIEGWFPYMLALAFLLVRPEGLFGEKHIDRV"
BASE COUNT          333 a          634 c          559 g          228 t
ORIGIN      
        1 gacgagttgt tggagaacca cggaaacttc accagcgccc acacgatgaa gaactgggcc
       61 gccagcgtcg ccaccgccag gtagaagccc ttgatgcgca ggctcggcag gccgaacacg
      121 atgcccaccg ccgccgccgt cagcccggcc agcaccagcg ccaccagaaa gggcatgccc
      181 tcgacgcgca ggatgaagtt gtaggccgca aacgccccca ccgccatgaa ggccgccgaa
      241 ccgagggaca gctgccccgc gtagcccgtg aggatgttca gcccgatggc cgccagagaa
      301 aagatcagca ccggcacgag gatcgccttc agccagtact ggtcggcgat caccggcacc
      361 gccacgccga acaccagcat catcagccag aagccgacgc ggtcctggcg gatcggaaag
      421 atctgcgcat cctcggcata cgtggccttg aactggccgg cctcacgata aaacatgcgt
      481 gtcgctcctt gcggcaatca gcgcttcaac gattctcagg acacgccgcg gcaaacgccg
      541 ccagcgtggg ggcggtcaag ctcagacacg gtcgatgtgc ttctcgccga acagcccctc
      601 gggccgcacc agcaggaagg ccagggccag catgtagggg aaccagccct cgatgccgcc
      661 gcccaccagc ggacccacgt acacctcggc cagcttctcc gacgcgccga tgatcagccc
      721 gcccacgatg gcccccggca ccgaggtgaa gccgcccagg atcagcaccg gcagcgcctt
      781 cagcgcggtg aaggtgaggg cgaactgcac gccgttgcgg gcgccccaga tgaggcccgc
      841 caccagcgcc acgaagcccg ccaccgccca caccacgcgc cagatggtct gcagcgggat
      901 gccgatggac agggccgcct ggtgatcgtc cgccaccgcc cgcagggcgc ggcccacctt
      961 ggtgtattgg aagagcagcg ccagcaaggt caccagcgag gccgccacgc cggcggcgaa
     1021 gaggtcgaac ttggagacgc cgatctcgta ggtgtccatc agccaggcga tgggctcatc
     1081 cacgatgccc aggtccaggc cgcgcacgtt ggcgccccag gtcatctggg ccagaccttc
     1141 caccacgtag gtgaggccga tggtggccat gaagagggtg atgtggggct gattcaccag
     1201 cgggcgcagc acgatgcgct cggtggcgat gcccagcacc accatcaccc cgaaggccag
     1261 caggaaggac agccagaaca ccgccgagcc gtccgccgcc aggcccaggt gggcgggcag
     1321 cagctcctga aagcccacga aggtcagcgc ggcgaagaac accattgccc cctgggcaaa
     1381 gttgaacacg cccgaggcct tgtagatcag cacgaagccc agcgccacca gggcatacat
     1441 caccccggag agcaggccgc cgatcagcac ctccagaaag aattgcatcg ttgtcgtctc
     1501 ccccgcgccg ggctggcgcg ctgcaagtcc gccgcgggcg gtgtggttcc atgggtggcg
     1561 gtcgggtccg tcgccctgcg ccgtgtcggg tgctgcctgc atcgcggcgg tgcggagcgg
     1621 cccgtacggc gccgcgccgc cctgcctccg gccgaagccg gcgttcattt ctcccacctt
     1681 ccggcgcgtc gtggcgcagc cttgtcttca tgggccgatc ggccttggcc gtccgcctcg
     1741 tttcactgcc tctt
//
LOCUS       DAOQPA010000266         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_68928_length_1727_cov_20.564593, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000266 DAOQPA010000000
VERSION     DAOQPA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68928_length_1727_cov_20.564593"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKX01_17720"
     CDS             <1..486
                     /locus_tag="PKX01_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HMZ78029.1"
                     /translation="TAPRPVLPAPAPAAGAALRSGGTPAYDAPALIHASIAQRPAVAA
                     PSPSLQAAALAALDDWLSAWSRRDVEQYLARYASDFRPAGGRSRAGWEAERRQRIGQA
                     VHIEVRAEAPQVEVLGPEQVRVVFRQHYRSDAYAGVDAKRLTLVLQGGRWLIQREESG
                     R"
     gene            568..>1727
                     /locus_tag="PKX01_17725"
     CDS             568..>1727
                     /locus_tag="PKX01_17725"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HMZ78030.1"
                     /translation="MAAVIARPPHRRRPVVEELEARILYAADLAPFVHPLTDGAQVRV
                     LEAPAATPAPTLVEQAATTRHEIVFVDPGVADWTRLVADLQAQQDADRQLDVVLLDAG
                     RDGVAQITGALQGLHDIAAIHLISHGEAGAVMLGSTRLDLASIDAEAARIADWAPALQ
                     AGADILVYGCDVAASADGQALLARLRALSGADIAASTDLTGAARLGGDWTLEYSLGAI
                     QARVAADVPTQADWQGVLATYTVTNTNDSGAGSFRQAIQDANANPGADTINFNIAGTG
                     IHTITLSSMLPYLSGQVNIDATTDDSFAANGNRPAIAIDGGNTIQDGLELYGGSDGSV
                     VRGLIIERFTQDGIDIASSGNNTVAGNWIGLNTAGTAAAGNQQGVNIWSANN"
BASE COUNT          255 a          611 c          606 g          255 t
ORIGIN      
        1 acagccccgc gcccggtgct gcccgcgccg gcgccggcgg ccggggccgc gctccggtcg
       61 ggggggactc ctgcgtatga tgcgcctgcc ctgatccacg catccatcgc gcagcgtccg
      121 gccgtcgcgg caccctcgcc gtcgctgcag gcggcggccc tggcggccct cgacgactgg
      181 ctgtccgcct ggtcacgacg ggatgtggag cagtatctcg cgcgctatgc cagcgacttc
      241 cggccggccg gtggccgttc acgggccggg tgggaagcgg aacgtcgtca gcgtatcggg
      301 caggcggtgc acatcgaggt gcgcgccgag gcgccccagg tcgaggtgct cgggccggag
      361 caggtccgtg tcgtctttcg ccaacactat cgctcagacg cctatgcggg cgtggacgcc
      421 aagcgcctca ccctggtcct gcaggggggg cgctggctga tccagcggga agagagcggg
      481 cgttgagatg aagggccgga tggcatgcgt accgatctgc ggggtcggcc tcccggacgc
      541 cgtggggcgt gaaggcggag ctgtggcatg gccgctgtga tcgcccgccc gccccatcgc
      601 cggcggccgg tcgtggagga gctggaggcc cgcatcctct acgccgccga tctggcgccc
      661 ttcgtccatc cgctcaccga cggcgcccag gtccgggtcc ttgaggcgcc ggccgcgacg
      721 ccggcgccca cgctggtgga acaggccgcc acgacccgcc acgagatcgt cttcgtcgac
      781 cctggcgtcg ctgactggac ccggctggtg gccgatctgc aggcccagca ggatgccgac
      841 cggcagctcg acgtggtgct cctggacgcg gggcgcgacg gtgttgccca gatcaccggg
      901 gcgctgcagg gactgcacga cattgcggcc atccacctga tctcccacgg cgaggcgggc
      961 gcggtgatgc tcggctcgac ccgtctcgat ctcgccagca tcgacgccga ggcggcccgc
     1021 atcgcggact gggcgcccgc cctgcaggcc ggcgccgaca ttctggtgta tggctgtgat
     1081 gtggccgcct cggccgatgg ccaggccttg ctggcccgcc tgcgtgcgct gtcgggcgcc
     1141 gacattgccg ccagtaccga tctcacgggc gctgcccgac tggggggcga ctggaccctg
     1201 gaatactccc tcggggccat ccaggcccgg gtggcggctg acgtgccgac ccaggcggac
     1261 tggcagggcg tgctggccac ctataccgtc accaacacca acgattcggg cgccggctcg
     1321 ttccgccagg cgattcagga tgccaacgcc aatcccggcg cggacacgat caacttcaac
     1381 atcgccggca cgggcatcca caccatcacg ctgagttcca tgctgcctta cctctcgggg
     1441 caggtgaaca tcgacgccac caccgatgac agctttgccg ccaacggcaa ccggccggcc
     1501 atcgccatcg atgggggcaa caccatccag gatggtctgg agctgtacgg cggctccgac
     1561 ggcagcgtag tgcgcgggct gatcatcgaa cgcttcaccc aggacggcat cgacatcgcg
     1621 tcgagcggca acaacacggt ggcggggaac tggatcggtc ttaatactgc aggcaccgcc
     1681 gcagccggca atcagcaggg cgtcaatatc tggtcggcga acaacaa
//
LOCUS       DAOQPA010000267         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_70101_length_1712_cov_17.835244, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000267 DAOQPA010000000
VERSION     DAOQPA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70101_length_1712_cov_17.835244"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKX01_17730"
     CDS             complement(<1..561)
                     /locus_tag="PKX01_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HMZ78031.1"
                     /translation="MASTVRTRFAPSPTGYLHIGGARTALFSWAFARRHGGTFVLRIE
                     DTDVARSTPEAVQAILDGMSWLDLNWDEGPFYQMQRMDRYKAVIQAMLAAGTAYPCYM
                     PPEELDALREAQRARGEKPRYDGRWRPEPGKELPAPPVGVQPVIRFKNPTEGVVAWDD
                     LVKGRIEIANAEMDDLIIARADGTPTY"
     gene            complement(769..1107)
                     /locus_tag="PKX01_17735"
     CDS             complement(769..1107)
                     /locus_tag="PKX01_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMZ78032.1"
                     /translation="MKKIEAVIKPFKLDEVREALSEVGITGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEVIVSSDVAEQAIEAIIKAARTGKIGDGKIFVMPVEQVVRI
                     RTGETNESAI"
     gene            complement(1177..>1712)
                     /gene="nadE"
                     /locus_tag="PKX01_17740"
     CDS             complement(1177..>1712)
                     /gene="nadE"
                     /locus_tag="PKX01_17740"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HMZ78033.1"
                     /translation="DTTEENLQSRIRGMLLMALSNKTGAIVLTTGNKSEMATGYATLY
                     GDMAGGFAVIKDLYKTFVYRLCAWRNALHGQPVIPDNILTRPPSAELKPDQCDQDSLP
                     PYEVLDAIIAAYMEEDLSPAEIVARGYPEHDVRRTVAMLKRNEYKRRQAPPGVRVTQR
                     GFGKDWRYPITSRYRDA"
BASE COUNT          257 a          531 c          615 g          309 t
ORIGIN      
        1 gtaggtgggc gtgccgtcgg cgcgggcgat gatgaggtcg tccatctcgg cgttggcgat
       61 ctcgatgcgg cccttgacca ggtcgtccca cgccaccacg ccctcggtcg ggtttttaaa
      121 gcggatcacc ggctgcacgc cgacgggcgg cgcgggcagt tccttgccgg gctcgggccg
      181 ccagcggccg tcgtagcggg gcttttcgcc gcgggcgcgc tgggcttcgc gcagggcgtc
      241 cagttcttcc ggcggcatgt agcaggggta cgccgtgccg gcggcgagca ttgcctggat
      301 caccgccttg tagcggtcca tgcgctgcat ctggtagaag gggccctcgt cccagttcag
      361 atccagccag ctcatgccgt cgaggatggc ctgcaccgcc tcgggcgtgg agcgggccac
      421 gtcggtgtcc tcgatgcgca gcacgaaggt gccgccatga cggcgggcaa aggcccacga
      481 gaacagggcc gtgcgcgcgc ctccgatgtg caggtagcca gtggggctgg gggcgaagcg
      541 ggtgcggacg gtggaagcca tggtacagat ccggaaatgc tgaaaaggcg gcattctacg
      601 cgctgcgcca ggatcgcaaa atcggcccgg cgcgccgact cacccgcagg ccagcgggac
      661 gggacggctc gtgaacgaca atccccgccg cgcggacggc cgcggggcgg ggatcgggga
      721 agacccgttg cgggccccgc acatcggcgg ggcgaccgca ggcgacgctc agatcgccga
      781 ttcgttggtc tcgccggtac ggatgcgcac gacctgttcg acgggcatca cgaagatctt
      841 gccgtcaccg atcttgccgg tgcgggcggc cttgatgata gcctcgatgg cctgctcggc
      901 cacgtcggag gagacgatca cttccagctt gatcttgggc aggaaatcca ccacgtattc
      961 ggccccgcgg tacagctccg tgtggccctt ctggcgaccg aaacccttca cctcggtgac
     1021 ggtcaggccg gtgatgccca cttcggacag ggcttcccgg acttcgtcca gcttgaaggg
     1081 cttgatcacg gcttcgattt tcttcatggg cagtctcctg aaaacaattc ggcattttag
     1141 ccgcatttgc cggcccgcca aggggccggg cgggttttac gcgtcccggt accgggaggt
     1201 gatggggtaa cgccagtcct tgccgaagcc gcgctgggtc acgcgcacac ccggcggcgc
     1261 ctggcgccgt ttgtattcgt tgcgcttgag catggccacg gtgcggcgca cgtcgtgttc
     1321 cggatagccg cgggcgacga tctcggccgg cgacagatcc tcttccatgt aggccgcgat
     1381 gatggcgtca agcacctcgt agggcggcag ggagtcctga tcgcactggt cgggcttgag
     1441 ttcggcagag ggtggccggg tgaggatgtt gtccgggatc accggctggc cgtgcagcgc
     1501 gttgcgccat gcgcacagac gatagacgaa ggtcttgtag agatccttga tcacggcgaa
     1561 gccgcccgcc atgtcgccgt acagggtggc gtagccggtg gccatctcgc tcttgttgcc
     1621 ggtggtgagg acgatggcgc cggtcttgtt ggacagggcc atcagcagca tgccgcggat
     1681 gcgcgactgg aggttctcct cggtggtgtc ct
//
LOCUS       DAOQPA010000268         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_70860_length_1702_cov_15.865817, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000268 DAOQPA010000000
VERSION     DAOQPA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70860_length_1702_cov_15.865817"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKX01_17745"
     CDS             complement(<1..722)
                     /locus_tag="PKX01_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ78034.1"
                     /translation="MLDLADPFDLPTRAARARDVLARLADPPTAAQPLHRHTDGRILG
                     RWYGLALASAFQPIVDGHSGVPAGYEAFLRSASADEHALSPWPFFASAADDSRLIALD
                     RLARSLHLLNALAADLRVPLFLNVHGRLLASVADDHGRAFRRVVDAVGADPARIVIET
                     PVEASDQPDLLAFVLRNYRHHGFQVAVNLASPAQWARLAGSVWPQYLKISARALGEGD
                     AARAALGQLAAQREDATLIVTRV"
     gene            complement(969..>1702)
                     /gene="lipA"
                     /locus_tag="PKX01_17750"
     CDS             complement(969..>1702)
                     /gene="lipA"
                     /locus_tag="PKX01_17750"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HMZ78035.1"
                     /translation="CFGKGTATFMIMGDICTRRCPFCDVGHGRPEPLNPDEPGNLAKT
                     IAAMRLNYVVITSVDRDDLRDGGADHFVQCIRATREASPKTTIEVLVPDFRGRMDVAL
                     DLFDAAPPDVMNHNLETVPRLYRQARPGADYAYSLELLKRFKARHPEVNTKSGLMVGL
                     GETDDEILEVMRDLRAHDVDMLTIGQYLQPSGGHLPVLRYVHPDTFKMFETEALKMGF
                     KNAACGPMVRSSYWADQQAHGAGVV"
BASE COUNT          223 a          501 c          732 g          246 t
ORIGIN      
        1 acgcgggtga cgatcagggt ggcgtcttcc cgttgggcgg cgagctgccc cagggcggcg
       61 cgggcggcgt cgccttcgcc cagggcgcgt gcgctgatct tgaggtactg gggccacacg
      121 ctgccggcga ggcgcgccca ctgggccggc gaggccaggt tcacggcgac ctggaagccg
      181 tggtggcggt agttgcgcag cacgaaggcg aggaggtcgg gctggtcgct ggcctcgacg
      241 ggggtttcga tgacgatgcg cgcggggtcg gcgccgacgg cgtccaccac gcggcggaag
      301 gcgcggccgt ggtcgtcggc aacgctggcg aggagccggc cgtgcacgtt gaggaagagc
      361 ggcacccgca ggtcggccgc gagggcgttg aggagatgca ggctgcgggc gaggcgatcc
      421 agggcgatca ggcggctgtc gtcggcggcg ctggcgaaga agggccacgg cgacagggcg
      481 tgttcgtcgg ccgaggcgct gcgcaggaag gcctcgtagc cggccggcac gccgctgtgg
      541 ccgtccacga tgggctggaa ggcggaggcg agggccaggc cgtaccagcg gccgaggatg
      601 cgcccgtcgg tgtggcggtg cagcggctgc gcggccgtcg gcgggtcggc gaggcgggcg
      661 aggacgtcgc gggcacgggc ggcccgggtg ggcaggtcga aggggtcggc gaggtcgagc
      721 atgggcactc cgcaggggga tctcgggcgg cgccggaagc ggtccgcagc gccgactcta
      781 aggcgtgggc cggccggcgg gaaccaagat tttctgcgtt gcttatacgg cgggcgtgcg
      841 acggatcgtc gcggcgcggc gtgcggcgtg ttgaccgtgg agaggcgggg agacggcggg
      901 gtgtgggcgg gcgccggagc cgccccgcaa cacccgcggg cacgcgggcg gcggggcgtc
      961 ccgcgggctt acacgacccc ggcgccgtgg gcctgctggt cggcccaata ggagctgcgc
     1021 accatggggc cgcaggcggc gttcttgaag cccatcttca gggcttcggt ctcgaacatc
     1081 ttgaaggtgt cgggatggac gtagcgcagc accggcaggt ggccgccgga gggttgcagg
     1141 tactggccga tggtgagcat gtccacgtcg tgggcgcgca ggtcgcgcat gacctctagg
     1201 atctcgtcgt cggtctcgcc gaggccgacc atcaggccgg acttggtgtt gacctccggg
     1261 tgacgggcct tgaagcgctt gaggagctcc agggagtagg cgtagtcggc gccggggcgg
     1321 gcctgcctgt agaggcgcgg cacggtttcg aggttgtggt tcatgacgtc cggcggggcg
     1381 gcgtcgaaga ggtccagggc cacgtccatg cgaccgcgga agtcgggcac cagcacctcg
     1441 atggtggtct tcgggctggc ctcgcgggtg gcgcggatgc actggacgaa gtgatcggcg
     1501 ccgccgtcgc gcaggtcgtc ccggtccacg ctggtgatca ccacgtagtt gaggcgcatc
     1561 gcggcgatgg tcttggcgag gttgcccggt tcgtccgggt tgaggggctc ggggcggccg
     1621 tggcccacgt cgcagaaggg gcagcggcgg gtgcagatgt cgcccatgat catgaaggtg
     1681 gccgtgccct tgccgaagca tt
//
LOCUS       DAOQPA010000269         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_72658_length_1680_cov_18.218462, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000269 DAOQPA010000000
VERSION     DAOQPA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72658_length_1680_cov_18.218462"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKX01_17755"
     CDS             <1..195
                     /locus_tag="PKX01_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ78036.1"
                     /translation="WRKLDVVQCGYCQSGQIMSAIALLNTHRAPSDADIDAALAGNLC
                     RCGTYVRIRAAIHEAAKALA"
     gene            206..>1680
                     /locus_tag="PKX01_17760"
     CDS             206..>1680
                     /locus_tag="PKX01_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ78037.1"
                     /translation="MSHSIHNVSRRQFLQGAAGLSLGLALPAFGARIEAGPGKAGMMV
                     GEAAFEPNAFLRIGVDNSVTVISKHLEMGQGTYTGLATLVAEELDAAWSQVKVEGAPA
                     DAKRYNNLFWGPAQGTGGSTAMANSWEQMRKAGAAARAMLVNAAAKRWSVPAGEITVR
                     DGLIQHAPSRRSAGFGEMAVAAGAEAVPASVTLKDPRDFRLIGRQAPRKDSAAKTNGT
                     AQFTQDLHLPDMLVAVVAHPPRFGATVKSVDAKAARAVKGVVDVVTIPTGVAVLARTT
                     WSARKGRDALKIDWDESAAFRLGSDEILARYRDLARTPGLVARKEGDVGAALAGARKV
                     ITADYDFPYLAHAAMEPMNCVVQRTAAGVEVWNGEQLHTGDQHALAALFGVAVDDVNI
                     HMLYAGGSFGRRACKDSDYVREAASIVKAAGTQAPVKLVWFREDDMRAGYYRPLFHHR
                     LEAAVDEGGRIVAWRHRLVGQSIIKGSPFESFLIKDGIDAVS"
BASE COUNT          261 a          575 c          598 g          246 t
ORIGIN      
        1 tggcgcaagc tggacgtggt ccagtgcggc tactgccagt ccggccagat catgagcgcc
       61 atcgccctgc tgaataccca ccgcgcgccc agcgacgcgg acatcgatgc cgccctggcc
      121 ggcaacctgt gtcgctgcgg tacctacgtg cgcatccgcg ccgccatcca cgaagccgcc
      181 aaggcgctgg cctgaggaga tcgcgatgag ccattcgatc cacaacgtct cccgccgcca
      241 gttcctgcaa ggggccgccg gtctcagcct gggcctcgcc ctgcccgcct tcggtgcccg
      301 catcgaagcc ggtcccggta aggccggcat gatggtcggc gaggcggcct tcgagcccaa
      361 cgccttcctg cgcatcggcg tggacaacag cgtcacggtg atctccaagc acctggagat
      421 ggggcagggc acctatacgg gcctggccac cctggtggcc gaggaactgg acgcagcctg
      481 gtcccaggtg aaggtggaag gtgcgccggc cgacgccaag cgctataaca acctgttctg
      541 gggcccggcc cagggcacgg gcggcagtac cgccatggcc aattcctggg agcagatgcg
      601 caaggccggc gccgccgccc gcgccatgct ggtgaatgcc gcggccaagc gctggtccgt
      661 gccggcgggg gagatcaccg tccgggacgg gctgatccag cacgcgccga gccggcgcag
      721 cgcgggtttc ggcgagatgg ccgtggcggc cggcgccgag gccgtgccgg cgagcgtcac
      781 cctcaaggac cccagggatt tccgcctgat cggccgccag gcgccgcgca aggacagtgc
      841 cgccaagacc aatggaacgg cccaatttac ccaggatttg catctgccgg acatgctcgt
      901 ggcggtggtg gcgcacccgc cgcgctttgg cgccacggtg aagtccgtcg acgccaaggc
      961 ggcccgcgcc gtgaagggcg tggtggacgt ggtgacgatc ccgactggcg tggcggtgct
     1021 ggcccggacc acctggagcg cccgcaaggg gcgcgacgcg ctgaagatcg actgggacga
     1081 aagcgcggcc ttccgtctgg gcagcgacga gatcctcgcc cgctaccggg atctggcccg
     1141 cacgcccggt ctggtcgccc gcaaggaggg cgacgtgggg gccgcgctgg ccggcgcccg
     1201 caaggtgatc accgccgatt acgactttcc ctacctggcc cacgctgcca tggagcccat
     1261 gaattgcgtc gtgcagcgca ccgcggctgg tgtggaggtg tggaacggcg agcagctcca
     1321 cacgggtgac cagcatgccc tggcggcgct gttcggggtg gcggtggatg acgtgaacat
     1381 ccacatgctc tacgccggcg gcagcttcgg ccggcgggcg tgcaaggatt cggactacgt
     1441 cagggaggcg gcttccatcg tcaaggccgc gggcacgcag gcgccggtca agctggtgtg
     1501 gttccgcgag gacgacatgc gggcgggcta ctaccggccg ctcttccacc accgtctcga
     1561 agcggctgtg gacgagggtg ggcgcatcgt cgcctggcgt catcgcctgg tgggccagtc
     1621 catcatcaag ggctcgccct tcgagtcctt cctgatcaag gacggcatcg acgccgtgtc
//
LOCUS       DAOQPA010000270         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_72913_length_1677_cov_15.965475, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000270 DAOQPA010000000
VERSION     DAOQPA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72913_length_1677_cov_15.965475"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1406)
                     /locus_tag="PKX01_17765"
     CDS             complement(45..1406)
                     /locus_tag="PKX01_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HMZ78038.1"
                     /translation="MAARNRSLNLKPGRARQVLTLTHPNAAGIDIGSASHYVAVPPDR
                     DDEPVREFKSFTADLHALADWLQACGVDTVAMESTGVYWIPLYELLDRRGFTVLLVNA
                     RHVKNVSGRKSDVLDCQWLQQLMTYGLLRGAFRPADAVCALRSLSRQREMLLKSQGRQ
                     VQHMQKALTQMNIQLANVISDVVGETGQKILRAIVAGERDGQVLAAMKNVRIRATTEE
                     IAKSLEGNWRAEHLFALKQALAAFDFIGTQLAECDCELAAQLASLQAHAGTPAKGKRR
                     GRARNAPKFDLRAQLFQMCGVDLTRIDGIDVTTALVVVSEVGTDMSRFPTVKHFACWL
                     GLCPGTKITGGKVLSGKTKRCANRAAQALRLAAAALRSSQSALGAYFRRMCARMDKPK
                     AVTAAAHKLARLIYTMLTKGEEYTDQGQDYYEERYRQRVLRQLSQRAEKLGMKLVVSE
                     QPA"
BASE COUNT          276 a          563 c          522 g          316 t
ORIGIN      
        1 ctctcaagaa acacattcca ggtgactgat cggaaatgag attttcaagc gggttgttcg
       61 ctgacgacga gtttcatgcc cagtttttcg gcccgctggg acagttgccg caggacccgc
      121 tggcggtagc gttcctcgta gtagtcctgc ccctggtcgg tgtattcctc gcccttggtc
      181 agcatcgtat agatcagccg ggccagcttg tgcgccgccg ccgtcacggc cttgggcttg
      241 tccatgcggg cgcacatccg ccggaagtag gcacccagcg ccgactggct gctgcgcagc
      301 gccgcggctg ccaatcgcag ggcctgggcg gcacggttgg cacagcgctt agtcttgccg
      361 ctcaacacct tgccgccggt gatcttggtc cctgggcaca agcccagcca acaggcgaaa
      421 tgcttgaccg tggggaagcg ggacatgtct gtgcccacct cggaaacgac gacgagggct
      481 gtggtcacgt cgatgccatc gatgcgggtc agatccaccc cgcacatctg gaagagctgg
      541 gctctgaggt cgaacttggg ggcattgcgg gcgcggccgc gccgcttgcc cttggccggg
      601 gtccctgcat gcgcctgcaa gctggccagt tgcgccgcga gttcgcagtc gcattcggcc
      661 aactgcgtgc cgatgaaatc gaaggccgcg agggcctgct tgagggcaaa caggtgctcg
      721 gcgcgccagt tgccctccag gctcttggcg atctcctcgg tcgtcgcccg gatgcgcacg
      781 ttcttcatcg ccgccagcac ctgcccgtcg cgctccccgg ccacgatggc gcgcagaatc
      841 ttctgccccg tctcgcccac tacgtcggaa atcacgttgg ccagttggat gttcatctgg
      901 gtcagcgcct tttgcatgtg ctgtacctgc cgaccctggc tcttcaggag catctcgcgc
      961 tggcgcgaca gcgagcgcaa ggcgcagacg gcgtcggccg ggcggaacgc gccccgcaac
     1021 aacccgtaag tcatcagctg ctgcagccat tggcagtcga gcacgtcgga cttgcgcccc
     1081 gagacgttct tgacgtggcg ggcattgacc agcaagaccg taaagccgcg ccggtccagc
     1141 agttcgtaca ggggaatcca atagacgccg gtcgattcca tcgccaccgt atcgacgccg
     1201 caggcctgca gccagtcggc cagggcatga aggtcggcgg tgaaactctt gaactcgcgc
     1261 accggctcgt cgtcccgatc cggcggcacc gccacatagt gcgatgcgct gccgatatcg
     1321 atcccggcag cattggggtg ggtgagcgtc aggacctggc gcgcgcggcc aggtttgagg
     1381 ttcagactgc gattgcgtgc ggccatggca tcctccatca tccggattgg aatgtggcgc
     1441 cgcatcgggt acgtcgtcgt gctcactctc tcaaacggga tatcggcccg gcggctgcac
     1501 accgccgaac cgattcacca gtgtcgatga cgtcacccag gaccacgcta acccgcgggc
     1561 aatctgcacc attgccattc cggtcttccg cagcgccgca ttccaccttg ccatacagcg
     1621 cccgccccgt gtttcttcgg gacgatttgc ggccgcggcc cgattacccc gctaacc
//
LOCUS       DAOQPA010000271         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_76011_length_1640_cov_15.364669, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000271 DAOQPA010000000
VERSION     DAOQPA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76011_length_1640_cov_15.364669"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1268
                     /locus_tag="PKX01_17770"
     CDS             87..1268
                     /locus_tag="PKX01_17770"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HMZ78039.1"
                     /translation="MDALARFARRYLFADAAPLSPAERRRSALAALLGVLIFEGVLAV
                     LPVAAETRRLLAPLGATSVILFTLPHSPLAQPWSVAGGLFLPALAGSLCGASGLPGWA
                     AMGLAVAVSVWLMGRLRCIHPPGGAMALVMTLATLQGAPVAIGLAAAAWNILAILMAA
                     VAVNNLIPGRRYPACTPPPATPRVVRREAAGIRHPDLAAALAEMDGYLDVSEEELTDL
                     FQRAARHAFQRHVLLTCGDILEPAPPCIEFATGLNDAWRLMSHHAAGALPVVDRARRV
                     IGLLGLEDFLRHVTPDAGPRIGDNVRRLLRPTPASHSDKPEVAGQIMFTPAHGLVTVR
                     SDDSIAVAARLLSQPGPQRLLPVTDAAGKLAGVITPTGLTAVLFQHGALVDLRDASPV
                     A"
     gene            complement(1278..1544)
                     /locus_tag="PKX01_17775"
     CDS             complement(1278..1544)
                     /locus_tag="PKX01_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ78040.1"
                     /translation="MKTIHVCDHSTPEALYPFDARGVAKRFRHAAIFGALDALLPGET
                     MRFCNDHDPLPLLAQLEQRYGAAVSIAYVQREPGAIVIDFARRA"
BASE COUNT          198 a          623 c          553 g          266 t
ORIGIN      
        1 tggccgatct gcacctgcat caagtttttt cctcgttccg gcctttacag tcagactgcc
       61 gattgctcac tccgccccga ccacccatgg acgccctggc ccgtttcgcc cgccgttacc
      121 tgttcgccga tgccgcgccc ctgtcgccgg ccgagcgccg ccgcagcgcc ctggccgccc
      181 tgctgggcgt gctgatcttc gaaggcgtgc tggccgtcct gccggtcgct gccgagaccc
      241 gccgcctgct cgcgcccctg ggggccacct cggtcatcct gttcacctta ccccacagtc
      301 ccctcgccca gccctggtcg gtggcgggcg ggctgttcct gccggccctg gccggcagcc
      361 tgtgcggcgc ctcgggccta cccggctggg cggcgatggg gctggcggtg gccgtctcgg
      421 tgtggctgat gggccgcctg cgctgcatcc acccacccgg cggcgccatg gccctggtga
      481 tgaccctggc caccctgcag ggcgcgcccg tggccatcgg cctggccgcc gcggcgtgga
      541 acatcctggc gatcctgatg gccgccgtgg cggtgaacaa cctgatcccc ggccgccgct
      601 acccggcctg cacgccaccg ccggccacgc cgcgggtggt gcgccgggag gccgccggca
      661 tccgccatcc ggacctggcg gcggccctcg ccgagatgga cggctacctg gacgtgagcg
      721 aagaggagct caccgacctc ttccagcgcg ccgcgcgcca cgccttccag cgtcatgtgc
      781 tgctcacctg cggcgacatc ctggagccgg cgccgccctg catcgagttc gccaccgggc
      841 tcaacgatgc ctggcgcctg atgagccatc acgccgccgg cgccctgccg gtggtggatc
      901 gggcccgccg ggtgatcggc ctgctcgggc tggaggactt cctgcgccac gtgacgccgg
      961 acgccggccc gcgcatcggc gacaacgtgc gccgcctgct gcgcccgacg ccggccagcc
     1021 attccgacaa gccggaagtg gccggccaga tcatgttcac gccggcccac ggcctcgtca
     1081 cggtgcgcag tgacgattcc atcgccgtcg ccgcccgcct cctgagccaa cccggccccc
     1141 agcgcctgct gccggtgacc gacgccgccg gcaagctggc cggcgtcatc accccgaccg
     1201 gcctgacggc ggtactcttc cagcacggtg cactggtcga cctccgtgac gcctcaccgg
     1261 tggcgtgagc cgccgcgtca ggcgcgcctg gcaaagtcga tgacgatggc gccgggctcg
     1321 cgctgcacgt aggcgatgga caccgccgcg ccgtagcgct gttccagctg ggccagcagc
     1381 ggcagcggat cgtggtcgtt gcagaagcgc atggtctcgc cgggcagcag cgcgtccagg
     1441 gcaccgaaga tggcggcgtg gcggaaccgc ttggccaccc cgcgggcgtc gaagggatag
     1501 agggcttccg gcgtggaatg gtcgcagacg tggatcgttt tcatggggac tcctggggtt
     1561 cgaaaaatcc cgactgtttt tatcgggtat cgccagtgtg ccgcccggtc gcttcagatt
     1621 ccttgatctg gatcgggttt
//
LOCUS       DAOQPA010000272         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_76356_length_1636_cov_20.917774, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000272 DAOQPA010000000
VERSION     DAOQPA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76356_length_1636_cov_20.917774"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1378)
                     /locus_tag="PKX01_17780"
     CDS             complement(44..1378)
                     /locus_tag="PKX01_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HMZ78041.1"
                     /translation="MAVRKRAVLELAHPNAAGIDIGSASHYVAVPADRDDESVREFKS
                     FTDDLERLADWLVACGIDTVAMESTGIYWIPLFELLESRGFTVYLVNARHVKNVSGRK
                     SDVLDCQWLQQLMSYGLLSGAFRPKDEICALRAVSRQRDMLLSYQARHVQHMQKALAQ
                     MNVQLANVISDIVGETGQKIVRAIIAGERDGARLAQMKNVRIRASEAEIAKSLRGNWR
                     EEHLFALRQAMALYDAYGERLAECDQQLEALLAALQPEAAGDPGKNRRRSRARNAPKF
                     DVRAYLFGMCGVDLTRINGIDTTTALKVISEVGPDLSRFKSAKHFTSWLGLCPGTKIS
                     GGKVLSGASKRTANRAAQALRMAAAALRSSQSALGAYYRRLCARLDRPKAITATAHKL
                     ARLIYTMLTKGTEYTDQGQDYYEERYRQRVMHHLAKRAEKLGFQLTPLAVTT"
BASE COUNT          300 a          527 c          485 g          324 t
ORIGIN      
        1 ctttcaagaa acacatccta actaattgaa agcttggatg gttctaagtg gtcacggcca
       61 ggggggtgag ttggaagccg agtttttcag cgcgcttggc gaggtggtgc atgacgcgct
      121 ggcggtaccg ttcctcgtaa tagtcctgcc cctggtccgt atattccgtt cccttggtca
      181 gcatcgtgta gatcaggcgg gccagtttat gggcggtggc ggtgatggcc ttgggccgat
      241 cgagacgggc gcacaggcga cggtagtagg cgcccagggc agactggctg gaacgcagag
      301 ccgcggcagc catccgcagg gcctgggccg cccggttggc ggtgcgcttg ctggcgccgg
      361 agaggacttt gcccccggaa atcttcgttc ccgggcacag ccccagccag gacgtgaagt
      421 gcttggcact cttgaagcga gacaggtcgg gaccaacctc ggaaatcact ttcagcgcag
      481 tcgtcgtgtc gatgccgttg atgcgggtca gatcgacgcc acacatcccg aacagatagg
      541 cccggacatc gaatttcggg gcattccggg cccgcgagcg gcgtcgattc ttgcccggat
      601 caccggccgc ttcgggctgc agcgcagcca gcagggcttc gagttgctga tcgcattcgg
      661 ccaggcgctc cccgtaggcg tcgtacaggg ccatcgcctg ccgcaaggca aacagatgct
      721 cttcacgcca gttgccacgc agggacttgg caatttccgc ctcgctcgcc cggatgcgca
      781 cattcttcat ctgggcgagt cgggccccgt cccgttcgcc cgcgataatg gcgcgcacga
      841 tcttttgccc agtctcgccg acgatgtcgg aaatcacgtt ggccagttgg acattcatct
      901 gcgccagggc cttctgcatg tgctggacat gccgcgcctg gtaggaaagc agcatgtccc
      961 gctgccgcga cacggcacgc agggcacaaa tctcgtcctt cggccggaag gcgcccgaga
     1021 gcagcccgta gctcatcaat tgttgcagcc attggcagtc gagcacgtcg gatttgcggc
     1081 ccgagacatt cttcacgtgc cgggcattga ccagatagac cgtgaaaccc cgcgattcca
     1141 ggagttcgaa gagcggtatc caatagatgc cggtcgattc catggccacc gtgtctatcc
     1201 cgcaggcgac cagccagtca gccagccgct ccaggtcatc ggtgaaactc ttgaattccc
     1261 gcaccgactc gtcgtcccgg tccgcaggca ccgccacata gtgactcgcc gaaccgatat
     1321 caatccccgc cgcattcgga tgggccagtt ccaaaaccgc tcgcttgcgt accgccatgg
     1381 cttcctccat gatcgtcatc ggaatgccgc gccggttcgg gtacgtcgtt gtactcactc
     1441 tttcaatcgg gatgaccgct tccacggtct caccactttc accgacgtga cccaggacca
     1501 cgctaacact cgggcaggct cgctccaatg gcagatcggt cttcgccggc gcggcattcc
     1561 accttaccgc agcagcacgc cccctacgtg tttcttgaaa acgccttgcg gccgaaaggc
     1621 cgattacttc gctaac
//
LOCUS       DAOQPA010000273         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_76544_length_1634_cov_17.696643, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000273 DAOQPA010000000
VERSION     DAOQPA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76544_length_1634_cov_17.696643"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1540)
                     /locus_tag="PKX01_17785"
     CDS             complement(185..1540)
                     /locus_tag="PKX01_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISKpn23 family transposase"
                     /protein_id="HMZ78042.1"
                     /translation="MDHPEGAGSKRADRVEFDRRVHVEFRGAQLSSDGGLLVMRELDD
                     ALGLSDLAAKALRDTRRGKNTVHRLDGLFRQSVFGRLAGYEDVNDADRLALDPVMRQV
                     VGGRAVDAQAASASQMGRFETETLAQPENRAALADLNGQWIDRFHDRNGLKYIVLDMD
                     SSVSPTHGDQEGAAWNGHFDCTCYHPNFLFNQFGMLERCALRHGNVHSADGWRDVLDP
                     VIARYAERDLCGRFFRADAAYAIPAIYERLEEARFFYAIRLPANNVLREKIAHRLTRP
                     VGRPSLTKVKRFYEDFEYQAASWDKERRVIAKIEWHPGELFPRVGFIVTNLPMEPDWV
                     VRFYNQRGTAEQHIKEGKYAFHWTRLSCRKFRDNEVRLQLHALAYNLAIFLRCIELPE
                     TMADWSLTSLQLKLIKIGARVVRHARAITFQLAEVAVTGPMVRAILAAIRRLRAPPLC
                     A"
BASE COUNT          298 a          517 c          517 g          302 t
ORIGIN      
        1 ttcgcctgaa tccgaccgca ggacaatcat tttccgtccc gagcggcgtc gtcgggcgcg
       61 caaggcgctg agacagggca gatcggcccg cgaagccgcc gtgttgtgcc ctggtggcgg
      121 tgtttttcag cgcagcggac agacccatcc tgccgttttc gttcagtttg ggcgtggatg
      181 gcggtcatgc gcatagcgga ggcgctcgca atcggcggat ggcggcgagg atggcgcgga
      241 ccatcggacc ggtgacggcc acctcggcca gctggaaggt gatggcgcgg gcatggcgga
      301 cgacacgtgc cccgatcttg atcagcttca gttgcaggct ggtcaacgac cagtcggcca
      361 tcgtctcggg caactcgatg cagcgcaaga agatggccag gttgtaggcc agcgcatgca
      421 gttgcagtcg cacctcattg tcgcggaact tccggcatga cagccgcgtc cagtggaagg
      481 catatttacc ttccttgatg tgctgctcgg cggtgccgcg ctggttatag aaccgcacca
      541 cccagtcggg ctccatcggc aggttggtga cgatgaagcc gacacgcggg aacagttcgc
      601 ccggatgcca ttcgatcttg gcgatcaccc ggcgttcctt gtcccaggat gccgcctgat
      661 actcgaagtc ttcgtagaag cgcttgacct tggtcagcga aggtcgcccg acagggcgcg
      721 tcagccgatg cgcgatcttc tcgcgcagga cgttgttcgc aggcagccgg atggcgtaga
      781 agaatcgggc ttcttccagc cgctcataga tcgcggggat cgcgtaggcg gcgtcagccc
      841 ggaagaaccg gccgcaaagg tcgcgctccg cgtagcgggc gatgacgggg tcgagaacgt
      901 cccgccagcc atcggcgctg tggacgttgc catggcgcaa ggcgcagcgc tccagcatgc
      961 cgaactggtt gaacaaaaag ttcgggtgat agcaggtgca gtcgaaatgc ccgttccagg
     1021 ccgcgccctc ctgatcgcca tgggtggggc tgaccgaact gtccatgtcc agaacgatgt
     1081 acttcagccc gttacggtca tggaaccggt cgatccattg gccattcagg tcggccagcg
     1141 cggcacggtt ctcgggctgt gccagtgtct cggtctcgaa ccgtcccatc tgcgaggccg
     1201 aggccgcttg cgcatcgaca gcccttccac ccacgacctg gcgcatcacc ggatcgaggg
     1261 ccaggcggtc ggcgtcgttc acatcctcgt atccggccag tcgcccgaac accgattgcc
     1321 ggaacaaccc gtcaagccgg tggaccgtgt tcttgccgcg ccgggtgtcg cgcaatgctt
     1381 ttgccgccag atcggacagc ccgagcgcgt catccagctc gcgcatcacc agcaggccgc
     1441 cgtcggaact gagctgcgca cctcggaact ccacatgcac ccggcggtcg aactcgaccc
     1501 gatctgcccg cttcgaaccc gcaccctctg ggtgatccat caaacgcgcc cctcacggcc
     1561 atcaacacca tgatttatat cggaaatatt gagatcctga cagcaaaatc agcgatttac
     1621 ttggggaatg tggg
//
LOCUS       DAOQPA010000274         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_82562_length_1571_cov_22.714380, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000274 DAOQPA010000000
VERSION     DAOQPA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82562_length_1571_cov_22.714380"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /locus_tag="PKX01_17790"
     CDS             complement(<1..>1571)
                     /locus_tag="PKX01_17790"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ78043.1"
                     /translation="GVRQQAFVFDGVDDYVSIPDSAVLDSLSTNATLDAWIKPEIPLG
                     AEAWLFARRDPFVSEGFSVALLQDGRIQLTVRTTTSPTVSGSTFRSTASVITFDQWQH
                     VAAAVDTVAGTAQIWVNGQAVSLTAVFGPATLSGTLFNVNQQYLGRRQDPNTAGGGGH
                     FKGEIDEVRLYGRTLTLAEVQSRAQTQPVATYVYDALSRRASLTLPNGIQTTYSYNPA
                     SQVTSILHQLTATGSQINKADYVYNPVGNRTSLTDRRGLQSFGYDQLDRLTSASHPLL
                     LDPQAFAYDAVGNRTMGGSTHNAGNQLTADANFAYQYDDNGNLIRKTLLATGNYTQYT
                     YDAENRLTKVEEFAAGNPTAITTSSYRYDGLGRRIEKVANGQTTRYIYDGEDILLEYN
                     GSNVLQARYTHGPGIDEPLSRTPMVPGLGSGQTAVLASTPDGLRGPVVDDGIKVNGQV
                     SVGFVFVSGMPAPAIGQPIDSTGLEQPIAPIDVTAAAATGTLAVDLIDTGGIGGNAPL
                     YLVTRDQATNQVLESR"
BASE COUNT          284 a          487 c          488 g          312 t
ORIGIN      
        1 cggctttcga gcacctggtt cgtggcctgg tcgcgcgtca ccagatagag cggagcattg
       61 cccccgatgc cccccgtatc gatcaggtcc accgcgaggg tgccagtggc cgccgcggcc
      121 gtaacatcga tcggcgcgat cggctgttca agaccggtcg agtcgatcgg ctgaccaatc
      181 gccggcgccg gcatcccgga gacgaagaca aagcctaccg acacctgacc gttcacttta
      241 atcccatcat ccaccaccgg cccccgcagg ccatcaggag tggaagccag caccgccgtc
      301 tgaccactgc cgaggcccgg caccatcggc gtccggctca agggctcatc gatgccaggg
      361 ccgtgggtat agcgggcctg gagcacgttg ctgccattat attccagcag aatatcttcg
      421 ccgtcgtaga tgtagcgtgt cgtctggccg ttggccacct tttcgatccg tcgccccaag
      481 ccatcatacc gatagctgct ggtcgtgatg gcagtcggat tcccagccgc aaactcctca
      541 accttggtca atcggttttc cgcatcatac gtgtactggg tgtagttacc cgtggcgagc
      601 agcgtttttc taatgaggtt cccattgtcg tcgtactggt aggcgaagtt cgcatcggcc
      661 gtgagttgat tgccggcatt gtgggtgctg ccgcccatcg tccgattccc caccgcatca
      721 taggcaaagg cttgtggatc gagtaacaac ggatggctcg cgctagtgag ccggtcaagc
      781 tgatcatagc cgaagctctg aagaccacgc cgatccgtca ggctggtccg attaccgacg
      841 ggattataga catagtcggc cttgttgatt tggctgccag tcgcggtgag ttgatgcagg
      901 atgctggtga cctgactggc gggattgtag ctgtaagtcg tctggatccc gttcgggagc
      961 gtgaggctgg cccgtcgcga gagggcgtca tagacatagg tcgccacggg ttgggtctgc
     1021 gcccggctct gcacctcagc cagcgtaagc gtccgcccat agagtcgcac ttcgtcaatc
     1081 tcgcccttga aatggccgcc cccacccgcc gtgttcgggt cttgccgtcg gccgagatac
     1141 tgttggttca cattgaagag ggtcccactc aaggtcgccg ggccgaagac ggcggtcagg
     1201 ctcacggcct gcccattcac ccagatctgc gccgtgcccg ccacggtatc cacggctgcg
     1261 gccacatgtt gccattggtc gaaggtgatc acgctcgcgg tggagcggaa cgtggagccg
     1321 gacacggtgg ggctggtggt ggtccggacg gtcaactgaa tccggccgtc ttggaggagc
     1381 gccacggaga acccttcgct cacgaaggga tcgcgccggg caaaaagcca ggcttcggcc
     1441 ccgaggggga tctccggctt gatccaggca tccagggtgg cgttggtgct gaggctatcg
     1501 agcaccgccg aatccgggat actcacatag tcatccaccc cgtcgaagac aaaggcctgc
     1561 tggcggacac c
//
LOCUS       DAOQPA010000275         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_84009_length_1557_cov_28.241012, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000275 DAOQPA010000000
VERSION     DAOQPA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84009_length_1557_cov_28.241012"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKX01_17795"
     CDS             complement(<1..767)
                     /locus_tag="PKX01_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ78044.1"
                     /translation="MRNLNYELKQLCRRNRDGSFATQRDRERVLDLVASQLHEMGYRH
                     MAAASLKPKHVEGLVERWQAEGLAVGTIKNRMAELRWWAEKIGKQNVIARDNDHYGIG
                     NRQYVTNVSKARQLTGGELARITDPYTAMSLRLQAAFGLRRGESIKIRPEWADRGDRL
                     ALKDTWTKGGRPREIPIRNAEQRQVLDEAKALAGRGSLIPAERSYVEQLRRFEYQCAA
                     AGAHRIHGHRHQYAQTRYRELTGWPAPAAGGPRSRDLT"
BASE COUNT          186 a          519 c          559 g          293 t
ORIGIN      
        1 gtgaggtctc tcgaccgcgg cccgccggcc gccggcgccg gccatccggt cagctcgcgg
       61 tagcgggtct gcgcgtactg atggcgatgg ccgtggatgc ggtgcgcgcc ggccgccgcg
      121 cactgatact cgaagcggcg caactgctcg acgtagcttc gctcggccgg gatgaggctg
      181 cccctgccgg ccagtgcctt ggcctcgtcg aggacctggc gctgctccgc gttgcggatc
      241 gggatctcgc gcggccgccc acccttggtc caggtgtcct tgagcgccag ccggtcgcca
      301 cggtcggccc actcgggacg gatcttgatc gactccccgc gccgcagccc gaaggccgcc
      361 tgcagccgca gcgacatcgc cgtgtacgga tccgtgatcc tggcgagctc gcccccggtc
      421 agttgccgcg ccttgctgac gttggtgacg tactgccggt tgccgatgcc gtagtggtcg
      481 ttgtcgcggg cgatgacgtt ctgcttgccg atcttctcgg cccaccatcg cagctccgcc
      541 atccggttct tgatcgtgcc cacggccagg ccttccgcct gccatcgctc caccaggccc
      601 tcgacgtgct tgggcttgag gcttgctgcg gccatgtgtc ggtagcccat ctcgtgaagc
      661 tgactcgcca ccaggtcgag cacgcgctcg cgatcgcgct gggtcgcgaa gctgccgtcg
      721 cggttgcgcc ggcacagctg cttcagctcg tagttcaggt tgcgcattcg gttgctgctg
      781 tcggtctccg tcgtcggttc ggtcggctgc ccctgggggc ggttcggatt cgttgtcgct
      841 ggtgtgtcgt cggtcaggtc agtgtttctg tccgtcccga acgcctgggc gttgcgggaa
      901 ccacgaggga cacgggacac cactcccgtt tcgtgccgag ttactgctgg ccgccgcggc
      961 acgcggcatt ggcgtcgatg gcgcttcctg cgggaaggcg atccatcggg tgagccattg
     1021 ccccaaggct ctgggtgcgc cggttggcgc catggctgac atggcccgcg gggagcgggc
     1081 gggtgcagac cgcgttgcac ggtccgaggt cttgacgcgc ctgggagccc gtcacgtggg
     1141 gtcgtcactt gcgacagccc cggcaaaacg ctcgtgcgct ggggcagcgt gcgcgtcgtc
     1201 actactggct ggcgccgcgt agtgacgacg cggttggcgc agaggtgctg gggcagggcg
     1261 cgggcgtgat tgcagcagtg gcgtctcggc tgatgccgct acggtcccgc atgctcgatt
     1321 ggggcagggc agacgcttcg tctccgaagc ctgacgcgta tctccagacc ttgcggccgc
     1381 cgggtttcag gagatgaggc cggcgtatgc gcccacgggt gcgggcaggg gcgatgcggt
     1441 gtcgggcatc agccgggctg actcagccag cgtaagcggg aacgatcctg cgccaacgtc
     1501 ttccggaccg caaccgcagt ggtcgactgc ggcaggcaca cgtcggccag gcattcg
//
LOCUS       DAOQPA010000276         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_86260_length_1536_cov_18.004727, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000276 DAOQPA010000000
VERSION     DAOQPA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86260_length_1536_cov_18.004727"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKX01_17800"
     CDS             complement(<1..1094)
                     /locus_tag="PKX01_17800"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HMZ78045.1"
                     /translation="MMTRIVLLVLDGFGIGSLPDAESYGDGGCNTLQRLAAISKGLAL
                     PNLEQLGLGLLGQFQGIRPMAQPEGCYGMLGFTTKGKHSLAGHWELAGYVIEDGPTPL
                     EAFTSELASGLEAALGQKTLGNCRTVHLEPIAEFGAQHQKSGMPIAWIDTAGTIMLAA
                     HEQVIPPEELYRLAREARRRLKATMPIVRVVAHPFTGQPGQFAITERRRDFAVEAPGL
                     TLLDHLSQASQLVFGVGKVGDLFSGRGVTRSVPLFQAHAVMDEVIGMFSKAPRGLIYA
                     NLPIMSPDLQDTLSTLQHVDRRLRDLQECLKVGDVLIITGDHGFDCARPNPGHSREYV
                     PCLVTGPRLARGVNLGTRTTAADLAQTIGEA"
     gene            complement(1183..>1536)
                     /gene="deoC"
                     /locus_tag="PKX01_17805"
     CDS             complement(1183..>1536)
                     /gene="deoC"
                     /locus_tag="PKX01_17805"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMZ78046.1"
                     /translation="QDMAAVVRATPGVNHKVILETCLLTREEKITACRLAVEAGMDYV
                     KTSTGFNQAGATVEDVRLMKEAVAGQAKVKASGGIRDWKSTRALLEAGADRIGTSASL
                     KILSEWRAEQAVMTR"
BASE COUNT          300 a          485 c          443 g          308 t
ORIGIN      
        1 gcttccccga tcgtctgagc aagatcggcg gccgtcgtcc tggtcccgag gttgacgcct
       61 ctggcaaggc gtggtcccgt caccaggcaa gggacatatt cgcgtgagtg tccgggattc
      121 ggtctggcgc agtcgaatcc gtggtcgccc gtgatgatca ggacatcgcc gactttgaga
      181 cactcttgca agtcgcgcaa ccggcgatcg acgtgttgca gcgtggacag ggtgtcctgc
      241 agatcggggc tcatgatagg gagattcgca tagatcaatc cgcgaggcgc tttgctgaac
      301 atgccgatga cttcgtccat cacggcatgc gcctgaaaca agggtaccga tctggtgacc
      361 ccgcgcccgc tgaaaaggtc gccgacttta cccaccccga ataccagctg gctggcttgg
      421 ctcaggtgat ccagcagtgt gaggcccggt gcttccacgg caaaatcccg gcgacgttcc
      481 gtaatagcaa attgtcccgg ctggccggta aacggatggg ccaccacgcg aacgatcggc
      541 atcgtcgcct tgagccggcg gcgagcctcg cgcgccaggc gatacaactc ctcaggcggg
      601 atgacctgtt cgtgggcggc cagcatgatg gtcccggcgg tatcgatcca cgcgatgggc
      661 atgcctgact tctgatgctg cgcgccgaac tctgcaatgg gctccaggtg aacggtgcga
      721 cagttcccca gcgtcttttg tcccaatgcg gcttcgagac cggaggccag ctcgctcgtg
      781 aacgcctcga gaggggtcgg accatcttcg atgacatacc cagccaattc ccaatgtccg
      841 gccagcgagt gtttaccctt ggtcgtaaac cccagcatgc cgtagcagcc ctccggttga
      901 gccatggggc ggataccttg aaattggccc aggagaccga ggccgagttg ttccagattc
      961 gggagggcaa gtcctttgga aatcgcagcc agccgctgca gcgtattgca gccgccatcg
     1021 ccatacgatt ccgcatcggg caaagacccg atgccaaatc catctaagac cagcaggacg
     1081 attcgagtca tcatcgaggc actatatcaa atccgctgaa acgagcaagc caatctcacc
     1141 gtccgagcac agtgacggcc gttccttcgt acccagtggg actcagcgag tcatgacggc
     1201 ctgttccgca cgccactcgc tcaggatttt cagactggcg ctggtgccga tccgatccgc
     1261 cccagcctcc agtaacgctc gtgtcgactt ccaatcccga atgccgccag aagcctttac
     1321 cttcgcctgt ccggccacgg cctctttcat gagccgcaca tcttccaccg tggcgccggc
     1381 ttggttgaat cccgtggagg tcttcacata gtccataccc gcttcaacgg ctaggcggca
     1441 cgccgtaatt ttttcttcgc gtgtgagcaa acaggtttcc agaatgacct tatggttgac
     1501 tccaggcgtg gcgcgcacga cggccgccat atcctg
//
LOCUS       DAOQPA010000277         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate AMR_MDS_0456
            NODE_86921_length_1530_cov_16.792542, whole genome shotgun
            sequence.
ACCESSION   DAOQPA010000277 DAOQPA010000000
VERSION     DAOQPA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810088
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (wastewater metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,562
            CDSs (total)                      :: 3,524
            Genes (coding)                    :: 3,517
            CDSs (with protein)               :: 3,517
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86921_length_1530_cov_16.792542"
                     /isolate="AMR_MDS_0456"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1456
                     /locus_tag="PKX01_17810"
     CDS             80..1456
                     /locus_tag="PKX01_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HMZ78047.1"
                     /translation="MMGERTVAQEALFYSFNLERHVPADHLLRSIDRFVDLSGIREHL
                     RPFYSSTGRPSVDPELMIRMLIVGYCMGIRSERRLCEEVHVNLAYRWFCRLGLEGDVP
                     DHSTFSKNRHGRFRDSDLLRQLFETTVARCIAEGLVGGEGFAVDGSLVRADVNRQHAV
                     DQADGLPDPATSHAVREYLAVLDDAAFGGATPVPPKQLAVADPAARWTAASRERAFFA
                     YATNYLIDLQHAVIVDVEATTAIRQAEVTAQRRMIDRTQERFGLWPERLVGDTGYGDA
                     KNLSWLVEERGIEPHIPVFDKSARRDDTFERSAFTFDHDDDSYTCPAGKRLRQSQRAY
                     SNARQSVDADGMLRYRASKLDCDACALKPRCCPTQPARKILRSVHEGARDLARDIATT
                     DAYLVSRRQRKKVEMLFAHLKRILRMDRLRLRGPNGARDEFLLAATAQNLRKMAKLIP
                     MPATPAPA"
BASE COUNT          270 a          497 c          469 g          294 t
ORIGIN      
        1 gattctgttg aaaaacttcc ccttgcaatg ctggtaaagt attgattcta tgtctctgca
       61 agcaagcgga gacgggcgta tgatgggtga gcggacggtg gcgcaggagg cgctgttcta
      121 cagcttcaac cttgagcggc atgttcctgc ggatcacctg ctgcgctcga ttgatcggtt
      181 cgttgacctg tcgggcattc gtgaacacct gcgccccttc tacagctcga cgggtcggcc
      241 ctcggtcgat cccgagctga tgatccggat gctgatcgtc ggctactgca tgggcattcg
      301 gtccgagcgg cggctgtgcg aggaggtgca tgtcaacctc gcctatcgtt ggttctgccg
      361 cctggggctg gaaggcgatg tgcccgacca ctcgaccttc tcgaagaacc ggcacgggcg
      421 cttccgcgac agtgatctgc tgcgccagct gttcgagacg accgtggcac gttgcatcgc
      481 cgagggactg gttggcggcg aagggttcgc tgtcgatggc agcctcgtgc gcgccgatgt
      541 gaaccgccag catgccgtgg atcaggcaga tggccttccg gatccggcca ccagccatgc
      601 cgttcgcgag tatctggccg tgctcgatga tgccgccttc gggggcgcaa ccccggtgcc
      661 gcccaagcag ttggcggtgg ctgatccggc tgcgcggtgg acagcggcaa gccgcgagcg
      721 ggccttcttt gcctatgcca ccaactatct gatcgatctt cagcatgcgg ttatcgtcga
      781 tgtcgaagcg actaccgcca tccgccaggc cgaagttact gcccagcgcc gcatgatcga
      841 ccggacgcag gagcgctttg gtctgtggcc tgagcggctt gtgggggaca ccggatatgg
      901 cgatgcaaag aacctgtcat ggctggtcga ggagcgcggc atcgagccgc acatcccggt
      961 gttcgacaag tctgcccgcc gcgatgatac cttcgagcgt tcggccttca ccttcgacca
     1021 tgacgatgac agctatacct gccctgctgg caagcgactg cgccagagcc agagggccta
     1081 cagcaatgcc cgtcagtcgg tcgatgccga cggcatgctg cgctaccgcg ccagcaagct
     1141 cgactgtgac gcctgcgctc tcaagccgcg ctgttgcccg acccagccag cacgcaaaat
     1201 cctgcgctca gtccacgagg gcgcccgtga tctcgcccgc gatatcgcca caaccgatgc
     1261 ttatctggtc tcgcgccgcc agcgcaagaa ggtcgagatg ctgttcgcac accttaagcg
     1321 catcctcaga atggatcgcc tgcgcctgcg aggtcccaac ggcgcaagag acgagttcct
     1381 gctcgccgcc accgcccaaa accttcgcaa gatggccaag ctgatcccga tgccagccac
     1441 cccagccccc gcttaagcgg gggcaaagcc tcagcgcggc ctcatgctcg cgccagcgcc
     1501 tgtctcagcg ccgggttttt caacacaatc
//
